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Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
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1

Milk lipid and protein traffic in mammary epithelial cells: joint and independent pathways  

E-Print Network [OSTI]

of prolactin and oxytocin on lipid and protein secretion suggest that these processes are coupled and co is not strictly required. milk lipids / mammary epithelial cells / secretion Résumé -- Transport des lipides et biologists, dairy scientists and technologists: Are the intracellular secretion processes of lipids

Boyer, Edmond

2

Interaction of mouse mammary epithelial cells with collagen substrata: regulation of casein gene expression and secretion  

Science Journals Connector (OSTI)

...polyvinyl pyrrolidone and then hybridized with 32P-labeled, nick-translated mouse caseins or whey acidic protein (WAP) cDNA...matrix of mammary cells (29) to that of casein gene expression merit further investigation. We thank Dr. Jeffrey M. Rosen for...

E Y Lee; W H Lee; C S Kaetzel; G Parry; M J Bissell

1985-01-01T23:59:59.000Z

3

Effects of Dietary Energy Restriction on Gene Regulation in Mammary Epithelial Cells  

Science Journals Connector (OSTI)

...Article Prevention Effects of Dietary Energy Restriction on Gene Regulation in Mammary...study was to determine whether dietary energy restriction (DER) affects the pattern of gene expression in three interrelated energy metabolism pathways: glycolysis, gluconeogenesis...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; Jennifer M. Price; Bifeng Gao; Henry J. Thompson

2007-12-15T23:59:59.000Z

4

Matrix Metalloproteinase Stromelysin-1 Triggers a Cascade of Molecular Alterations that leads to stable epithelial-to-Mesenchymal Conversion and a Premalignant Phenotype in Mammary Epithelial Cells  

E-Print Network [OSTI]

SL-1, and the cell line p2C, which was established in theshown. A fourth cell line, p2C, which was derived from SCp2Tet and SCp2 (not shown) and p2C cells maintained either in

Lochter, A.

2011-01-01T23:59:59.000Z

5

Association of Decreased Intercellular Communication with the Immortal but not the Tumorigenic Phenotype in Human Mammary Epithelial Cells  

Science Journals Connector (OSTI)

...per 100-mm dish were plated in medium containing 30 /J.MTG. After 3 wk, the plates were stained and TG' colonies containing...observed in the two cell lines is a function of the immortalization process. Previous observations using a variety of techniques to show...

Sandra R. Eldridge; Thomas W. Martens; Carol A. Sattler; and Michael N. Gould

1989-08-01T23:59:59.000Z

6

Carcinoma Induction following Direct in Situ Transfer of v-Ha-ras into Rat Mammary Epithelial Cells Using Replication-defective Retrovirus Vectors  

Science Journals Connector (OSTI)

...observed for chemically induced mammary car- cinogenesis (27, 28). Most of the...DISCUSSION We have established a replication-defective retrovirus vector- mediated gene transfer...Furthermore, as in chemically induced car cinomas, the frequency of mammary tumor...

Bingcheng Wang; Wendy S. Kennan; Jane Yasukawa-Barnes; Mary J. Lindstrom; and Michael N. Gould

1991-05-15T23:59:59.000Z

7

Persistence of gamma-H2AX and 53BP1 foci in proliferating and nonproliferating human mammary epithelial cells after exposure to gamma-rays or iron ions  

SciTech Connect (OSTI)

To investigate {gamma}-H2AX (phosphorylated histone H2AX) and 53BP1 (tumour protein 53 binding protein No. 1) foci formation and removal in proliferating and non-proliferating human mammary epithelial cells (HMEC) after exposure to sparsely and densely ionizing radiation under different cell culture conditions. HMEC cells were grown either as monolayers (2D) or in extracellular matrix to allow the formation of acinar structures in vitro (3D). Foci numbers were quantified by image analysis at various time points after exposure. Our results reveal that in non-proliferating cells under 2D and 3D cell culture conditions, iron-ion induced {gamma}-H2AX foci were still present at 72 h after exposure, although 53BP1 foci returned to control levels at 48 h. In contrast in proliferating HMEC, both {gamma}-H2AX and 53BP1 foci decreased to control levels during the 24-48 h time interval after irradiation under 2D conditions. Foci numbers decreased faster after {gamma}-ray irradiation and returned to control levels by 12 h regardless of marker, cell proliferation status, and cell culture condition. Conclusions: The disappearance of radiation induced {gamma}-H2AX and 53BP1 foci in HMEC have different dynamics that depend on radiation quality and proliferation status. Notably, the general patterns do not depend on the cell culture condition (2D versus 3D). We speculate that the persistent {gamma}-H2AX foci in iron-ion irradiated non-proliferating cells could be due to limited availability of double strand break (DSB) repair pathways in G0/G1-phase, or that repair of complex DSB requires replication or chromatin remodeling.

Groesser, Torsten; Chang, Hang; Fontenay, Gerald; Chen, James; Costes, Sylvain V.; Barcellos-Hoff, Mary Helen; Parvin, Bahram; Rydberg, Bjorn

2010-12-22T23:59:59.000Z

8

EGF-receptor phosphorylation and downstream signaling are activated by benzo[a]pyrene 3,6-quinone and benzo[a]pyrene 1,6-quinone in human mammary epithelial cells  

SciTech Connect (OSTI)

Benzo[a]pyrene (BaP) is activated by xenobiotic-metabolizing enzymes to highly mutagenic and carcinogenic metabolites. Previous studies in this laboratory have shown that benzo[a]pyrene quinones (BPQs), 1,6-BPQ and 3,6-BPQ, are able to induce epidermal growth factor receptor (EGFR) cell signaling through the production of reactive oxygen species. Recently, we have reported that BPQs have the potential to induce the expression of genes involved in numerous pathways associated with cell proliferation and survival in human mammary epithelial cells. In the present study we demonstrated that BPQs not only induced EGFR tyrosine autophosphorylation, but also induced EGFR-dependent tyrosine phosphorylation of phospholipase C-{gamma}1 and several signal transducers and activators of transcription (STATs). The effects of BPQs were evaluated in a model of EGF withdrawal in MCF10-A cells. We found that BPQs (1 {mu}M), induced EGFR tyrosine phosphorylation at positions Y845, Y992, Y1068, and Y1086. PLC-{gamma}1 phosphorylation correlated with the phosphorylation of tyrosine-Y992, a proposed docking site for PLC-{gamma}1 on the EGFR. Additionally, we found that BPQs induced the activation of STAT-1, STAT-3, STAT-5a and STAT-5b. STAT5 was shown to translocate to the nucleus following 3,6-BPQ and 1,6-BPQ exposures. Although the patterns of phosphorylation at EGFR, PLC-{gamma}1 and STATs were quite similar to those induced by EGF, an important difference between BPQ-mediated signaling of the EGFR was observed. Signaling produced by EGF ligand produced a rapid disappearance of EGFR from the cell surface, whereas BPQ signaling maintained EGFR receptors on the cell membrane. Thus, the results of these studies show that 1,6-BPQ and 3,6-BPQ can produce early events as evidenced by EGFR expression, and a prolonged transactivation of EGFR leading to downstream cell signaling pathways.

Rodriguez-Fragoso, Lourdes [Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Avenida Universidad 1001 Col. Chamilpa, Cuernavaca 62210, Morelos (Mexico); Melendez, Karla; Hudson, Laurie G.; Lauer, Fredine T. [University of New Mexico, College of Pharmacy Toxicology Program, Albuquerque, New Mexico (United States); Burchiel, Scott W. [University of New Mexico, College of Pharmacy Toxicology Program, Albuquerque, New Mexico (United States)], E-mail: sburchiel@salud.unm.edu

2009-03-15T23:59:59.000Z

9

Of Microenvironments and Mammary Stem Cells  

SciTech Connect (OSTI)

In most adult tissues there reside pools of stem and progenitor cells inside specialized microenvironments referred to as niches. The niche protects the stem cells from inappropriate expansion and directs their critical functions. Thus guided, stem cells are able to maintain tissue homeostasis throughout the ebb and flow of metabolic and physical demands encountered over a lifetime. Indeed, a pool of stem cells maintains mammary gland structure throughout development, and responds to the physiological demands associated with pregnancy. This review discusses how stem cells were identified in both human and mouse mammary glands; each requiring different techniques that were determined by differing biological needs and ethical constraints. These studies together create a robust portrait of mammary gland biology and identify the location of the stem cell niche, elucidate a developmental hierarchy, and suggest how the niche might be manipulated for therapeutic benefit.

LaBarge, Mark A; Petersen, Ole W; Bissell, Mina J

2007-06-01T23:59:59.000Z

10

Hepatocyte Growth Factor and Neuregulin in Mammary Gland Cell Morphogenesis  

Science Journals Connector (OSTI)

Organ culture and transplantation experiments in the early 1960s and 1970s have demonstrated that growth and morphogenesis of the epithelium of the mammary gland are controlled by mesenchymal-epithelial intera...

Catherin Niemann; 1; 2Volker Brinkmann; Walter Birchmeier

2002-01-01T23:59:59.000Z

11

Stromal-epithelial interactions in aging and cancer: Senescent fibroblasts alter epithelial cell differentiation  

SciTech Connect (OSTI)

Cellular senescence suppresses cancer by arresting cells at risk for malignant tumorigenesis. However, senescent cells also secrete molecules that can stimulate premalignant cells to proliferate and form tumors, suggesting the senescence response is antagonistically pleiotropic. We show that premalignant mammary epithelial cells exposed to senescent human fibroblasts in mice irreversibly lose differentiated properties, become invasive and undergo full malignant transformation. Moreover, using cultured mouse or human fibroblasts and non-malignant breast epithelial cells, we show that senescent fibroblasts disrupt epithelial alveolar morphogenesis, functional differentiation, and branching morphogenesis. Further, we identify MMP-3 as the major factor responsible for the effects of senescent fibroblasts on branching morphogenesis. Our findings support the idea that senescent cells contribute to age-related pathology, including cancer, and describe a new property of senescent fibroblasts--the ability to alter epithelial differentiation--that might also explain the loss of tissue function and organization that is a hallmark of aging.

Parrinello, Simona; Coppe, Jean-Philippe; Krtolica, Ana; Campisi, Judith

2004-07-14T23:59:59.000Z

12

Regulation of Mammary Lactogenic Differentiation by Singleminded-2s  

E-Print Network [OSTI]

juvenile gland showing TEB structures. H. WM mature mammary gland. I, WM mammary gland at pregnancy day 10. J, WM mammary gland during lactation. 3 Studies performed in mice lacking ESR1, ESR2, Pgr, GHR, or PrlR revealed that embryonic... tumor necrosis factor (TNF) ligand superfamily member, and its receptor, EdaR, are expressed in the mesenchyme and epithelial placode cells, respectively (Pispa et al. 2003). Overexpression of Eda in mice results in supernumerary and enlarged mammary...

Wellberg, Elizabeth

2010-07-14T23:59:59.000Z

13

Morphological and Histological Characteristics of Mammary Dysplasias Occurring in Cell Dissociation-derived Murine Mammary Outgrowths  

Science Journals Connector (OSTI)

...Smithville, Texas 78957 [L. M. A.]; and Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 [R. L U.] ABSTRACT The morphological...mammary carcinoma in humans. Wellings and co-workers (17,18) have classified human mammary lesions...

Stephen P. Ethier; Laurel M. Adams; and Robert L. Ullrich

1984-10-01T23:59:59.000Z

14

Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells  

E-Print Network [OSTI]

18 and 19 (K18K19), vimentin (vim) and ?-sm actin (ASMA) inDako), and vimentin (VIM; MEDAC, GmbH). Other antibodiesa mAb against vimentin (VIM, IgG1). The secondary antibodies

Pechoux, Christine

2010-01-01T23:59:59.000Z

15

Disrupted epithelial morphogenesis and aberrant lineage commitment  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Disrupted epithelial morphogenesis and aberrant lineage commitment Disrupted epithelial morphogenesis and aberrant lineage commitment following radiation and TGFβ I. Fernandez-Garcia New York University School of Medicine Abstract In the present study, we evaluated cell lineage plasticity and its phenotypic effects in the non-malignant human mammary epithelial cell line MCF10A in response to ionizing radiation and TGFΒ. We have recently shown that the molecular profiles of mammary tumors and normal mammary gland from irradiated mice are distinct from those arising in unirradiated mice by virtue of enriched stem cell and progenitor markers. Consistent with this, we found that radiation (10-100 cGy) increases the mammary repopulation capacity in a functional and marker assays (Nguyen et al. Cancer Cell 2011). Some studies have shown that epithelial-mesenchymal transition (EMT)

16

Factors Influencing Expression of Mammary Ductal Dysplasia in Cell Dissociation-derived Murine Mammary Outgrowths  

Science Journals Connector (OSTI)

...Michigan 48201 [S. P. E. ,and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 [R. L U.J ABSTRACT The expression...lesions of the mammary ducts (9, 10). Several workers have reported the detection of mammary ductal...

Stephen P. Ethier and Robert L. Ullrich

1984-10-01T23:59:59.000Z

17

Pim-1 kinase expression during murine mammary development  

SciTech Connect (OSTI)

Pim-1 kinase phosphorylates substrates whose activities are linked to proliferation, survival, differentiation, and apoptosis. Although pim-1 is induced by hormones and cytokines, the hormonal control and contribution of Pim-1 to mammary gland development have not been evaluated. We examined Pim-1 expression in mammary cell lines, investigated whether Pim-1 levels could be altered in breast epithelia by mammogenic hormones, and evaluated Pim-1 expression during mammary development. We found that Pim-1 was elevated in most mammary carcinoma cell lines and progesterone increased Pim-1 protein to some extent in non-tumorigenic mammary epithelia. Pim-1 expression in situ was consistent with the documented profile of progesterone activity in mouse mammary glands. Pim-1 nuclear localization correlated with cytoplasmic distribution for its substrate, p21{sup CIP/Waf1}, and we found that Pim-1 and p21 associate in vitro. Our results suggest that Pim-1 expression may be regulated by progesterone during mammary development and Pim-1 associates with p21 in mammary epithelial cells.

Gapter, Leslie A. [Department of Pharmacy, Faculty of Science, National University of Singapore, 117543 (Singapore); School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4234 (United States); Magnuson, Nancy S. [School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4234 (United States); Ng, Ka-yun [Department of Pharmacy, Faculty of Science, National University of Singapore, 117543 (Singapore); Hosick, Howard L. [School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4234 (United States)]. E-mail: hosick@wsu.edu

2006-07-07T23:59:59.000Z

18

Low Dose Radiation Research Program: The Progeny of Irradiated Mammary  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Progeny of Irradiated Mammary Epithelial Cells Exhibit a Phenotype Progeny of Irradiated Mammary Epithelial Cells Exhibit a Phenotype Characteristic of Malignancy Mary H. Barcellos-Hoff, R.L. Henshall-Powell, M.J. Bissell, and B. Parvin Lawrence Berkeley National Laboratory, Life Sciences Division We have proposed that the ability of radiation to induce altered microenvironments affects the frequency and features of neoplastic progression. Thus, we have sought to characterize the irradiated microenvironment and determine how these events contribute to mammary carcinogenesis. By using imaging bioinformatics to analyze mouse and human models of breast cancer we have now examined cell adhesion molecules (CAMs) critical for tissue-specific organization and function. We found that 1) radiation-induced microenvironments can contribute to neoplastic potential

19

E-Print Network 3.0 - anogenital epithelial cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

185-194 Plasticity of epithelial cell shape in response... expresses enhanced green fluorescent protein (eGFP) specifically in the ectodermal epithelial cells... to analyze the...

20

Assessment of Radiogenic Cancer Initiation Frequency per Clonogenic Rat Mammary Cell in Vivo  

Science Journals Connector (OSTI)

...X-rays from a General Electric Maxitron 300 with 3 mm Al...first and subsequent mammary car cinomas are not independent...D0 = 127 cGy low linear energy transfer irradiation and...differences in latencies of car cinomas from grafted cells...

Kelly H. Clifton; Martin A. Tanner; and Michael N. Gould

1986-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Disrupted epithelial morphogenesis and aberrant lineage commitment following radiation and TGFβ  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Disrupted epithelial morphogenesis and aberrant lineage Disrupted epithelial morphogenesis and aberrant lineage commitment following radiation and TGFβ I. Fernandez-Garcia and M.H. Barcellos-Hoff Department of Radiation Oncology, New York University School of Medicine, NY. In the present study, we evaluated cell lineage plasticity and its phenotypic effects in the non-malignant human mammary epithelial cell line MCF10A in response to ionizing radiation and TGFβ. We have recently shown that the molecular profiles of mammary tumors and normal mammary gland from irradiated mice are distinct from those arising in unirradiated mice by virtue of enriched stem cell and progenitor markers. Consistent with this, we found that radiation (10-100 cGy) increases the mammary repopulation capacity in a functional and marker assays (Nguyen et al. Cancer Cell

22

Probiotics promote endocytic allergen degradation in gut epithelial cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Knockdown of A20 compromised the epithelial barrier function. Black-Right-Pointing-Pointer The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Black-Right-Pointing-Pointer Antigens transported across A20-deficient HT-29 monolayers conserved antigenicity. Black-Right-Pointing-Pointer Probiotic proteins increased the expression of A20 in HT-29 cells. -- Abstract: Background and aims: Epithelial barrier dysfunction plays a critical role in the pathogenesis of allergic diseases; the mechanism is to be further understood. The ubiquitin E3 ligase A20 (A20) plays a role in the endocytic protein degradation in the cells. This study aims to elucidate the role of A20 in the maintenance of gut epithelial barrier function. Methods: Gut epithelial cell line, HT-29 cell, was cultured into monolayers to evaluate the barrier function in transwells. RNA interference was employed to knock down the A20 gene in HT-29 cells to test the role of A20 in the maintenance of epithelial barrier function. Probiotic derived proteins were extracted from the culture supernatants using to enhance the expression of A20 in HT-29 cells. Results: The results showed that the knockdown of A20 compromised the epithelial barrier function in HT-29 monolayers, mainly increased the intracellular permeability. The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Allergens collected from the transwell basal chambers of A20-deficient HT-29 monolayers still conserved functional antigenicity. Treating with probiotic derived proteins increased the expression of A20 in HT-29 cells and promote the barrier function. Conclusion: A20 plays an important role in the maintenance of epithelial barrier function as shown by HT-29 monolayer. Probiotic derived protein increases the expression of A20 and promote the HT-29 monolayer barrier function.

Song, Chun-Hua [Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou (China)] [Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou (China); Liu, Zhi-Qiang [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China) [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Huang, Shelly [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)] [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Zheng, Peng-Yuan, E-mail: medp7123@126.com [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China)] [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Yang, Ping-Chang, E-mail: yangp@mcmaster.ca [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)] [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)

2012-09-14T23:59:59.000Z

23

A tool for the quantitative spatial analysis of mammary gland epithelium  

SciTech Connect (OSTI)

In this paper we present a method for the spatial analysis of complex cellular systems based on a multiscale study of neighborhood relationships. A function to measure those relationships, M, is introduced. The refined Relative Neighborhood Graph is then presented as a method to establish vicinity relationships within layered cellular structures, and particularized to epithelial cell nuclei in the mammary gland. Finally, the method is illustrated with two examples that show interactions within one population of epithelial cells and between two different populations.

Ortiz de Solorzano, Carlos; Fernandez-Gonzalez, Rodrigo

2004-04-09T23:59:59.000Z

24

The low dose damage response pathways in the mouse mammary glands depends  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

low dose damage response pathways in the mouse mammary glands depends low dose damage response pathways in the mouse mammary glands depends on genotype, tissue compartment, exposure regimen, and sampling times Joe Gray & Andrew Wyrobek Lawrence Berkeley National Laboratory Abstract The objectives of this research are to characterize the early and persistent low-dose and adaptive response (AR) damage surveillance networks in mammary glands of radiation sensitive and resistant strains of mice to identify the molecular signatures/mechanisms associated with nonlinear modifications of risk for mammary gland cancer. Our approach uses low-dose exposure regimens that have been reported to induce mammary gland cancer in sensitive strains to determine whether low-dose induced pathways are differentially expressed in epithelial or stromal cells and to determine

25

Airway epithelial cell response to human metapneumovirus infection  

SciTech Connect (OSTI)

Human metapneumovirus (hMPV) is a major cause of lower respiratory tract infections (LRTIs) in infants, elderly and immunocompromised patients. In this study, we show that hMPV can infect in a similar manner epithelial cells representative of different tracts of the airways. hMPV-induced expression of chemokines IL-8 and RANTES in primary small alveolar epithelial cells (SAE) and in a human alveolar type II-like epithelial cell line (A549) was similar, suggesting that A549 cells can be used as a model to study lower airway epithelial cell responses to hMPV infection. A549 secreted a variety of CXC and CC chemokines, cytokines and type I interferons, following hMPV infection. hMPV was also a strong inducer of transcription factors belonging to nuclear factor (NF)-{kappa}B, interferon regulatory factors (IRFs) and signal transducers and activators of transcription (STATs) families, which are known to orchestrate the expression of inflammatory and immunomodulatory mediators.

Bao, X.; Liu, T.; Spetch, L.; Kolli, D. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States); Garofalo, R.P. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas (United States); Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas (United States); Casola, A. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas (United States); Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas (United States)], E-mail: ancasola@utmb.edu

2007-11-10T23:59:59.000Z

26

Bile Salts and Nuclear Receptors in Biliary Epithelial Cell Pathophysiology  

E-Print Network [OSTI]

of Michigan. This opportunity to complete a research program allowed me to pursue my career as an associate receptors 17 3. Research program 19 3.1. VDR in biliary-type liver diseases 20 3.2. VDR in fatty liverBile Salts and Nuclear Receptors in Biliary Epithelial Cell Pathophysiology by Dr. Nicolas Chignard

Boyer, Edmond

27

E-Print Network 3.0 - a549 epithelial cells Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

PROLIFERATION AND PROTEIN EXPRESSION OF LUNG CANCER CELLS Summary: (NSCLC) cell lines. Methods: The epithelial lung carcinoma (A549 ) and a bronchioalveolar (NCI-H358......

28

2-Deoxyglucose as an Energy Restriction Mimetic Agent: Effects on Mammary Carcinogenesis and on Mammary Tumor Cell Growth In vitro  

Science Journals Connector (OSTI)

...Thompson HJ. Effect of energy restriction on cell cycle...breast cancer research. New York: Kluwer Academic/Plenum...Thompson HJ. Mechanisms of energy restriction: effects of...nonparametric statistics. New York: John Wiley Sons; 1980...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; and Henry J. Thompson

2005-08-01T23:59:59.000Z

29

Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization  

E-Print Network [OSTI]

in the alveolar lumen with TEM are surrounded by a thin rim of plasma membrane-encased cytosol and the MFG membrane (Fig. 7a). This latter structure is acquired by the lipid droplet during the secretion process from the apical surface of the epithelium 13... for TEM analysis. Cell culture EpH4 cells 58 were maintained in DMEM (Life Technologies) media supplemented with 10% FCS (Sigma) at 37 C in a humidified atmosphere of 5% CO 2 . Milk collected from 24 h involuting mammary glands was incubated on cells...

Sargeant, Timothy J.; Lloyd-Lewis, Bethan; Resemann, Henrike K.; Ramos-Montoya, Antonio; Skepper, Jeremy; Watson, Christine J.

2014-10-05T23:59:59.000Z

30

Effects of Dietary Energy Restriction on Gene Regulation in Mammary Epithelial Cells  

Science Journals Connector (OSTI)

...pink, light blue, and dark blue) is directly related...carcinomas at the 10 false discovery rate was P 0.037...genes influencing the energy metabolism in MGEC and...MG-20 DER, 20 dietary energy-restricted MGECs; MG-40 DER, 40 dietary energy-restricted MGECs...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; Jennifer M. Price; Bifeng Gao; and Henry J. Thompson

2007-12-15T23:59:59.000Z

31

Effects of Dietary Energy Restriction on Gene Regulation in Mammary Epithelial Cells  

Science Journals Connector (OSTI)

...compounds, including energy, saturated fat...We measured diet quality with the HEI-2005...with better dietary quality were more physically...reported lower daily energy intake and weekly...components (40), is energy adjusted, facilitates...better versus poor quality diets, and can be...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; Jennifer M. Price; Bifeng Gao; and Henry J. Thompson

2007-12-15T23:59:59.000Z

32

Effects of Dietary Energy Restriction on Gene Regulation in Mammary Epithelial Cells  

Science Journals Connector (OSTI)

...Prevention Effects of Dietary Energy Restriction on Gene Regulation...determine whether dietary energy restriction (DER) affects...breast cancer research. New York: Kluwer Academic/Plenum...HJ. Effects of dietary energy repletion and IGF-1 infusion...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; Jennifer M. Price; Bifeng Gao; and Henry J. Thompson

2007-12-15T23:59:59.000Z

33

Selective growth of some rodent epithelial cells in a medium containing citrulline  

Science Journals Connector (OSTI)

...TENNANT, AND A. W. HSIE Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 Communicated by M. M. Rhoades...possessed by liver cells have enabled various workers to cultivate liver epithelial cells free of fibroblasts...

N C Sun; C R Sun; R W Tennant; A W Hsie

1979-01-01T23:59:59.000Z

34

Regulation of Mammary cell Differentiation and Metabolism by Singleminded-2s  

E-Print Network [OSTI]

mammary gland has also been shown to enhance metastasis, and the gene expression signature of the involuting gland has been associated with poor prognoses in breast cancer diagnosis (Marti et al., 1999; McDaniel et al., 2006; Schedin et al., 2007; Stein... profile similar to the involuting mammary gland (Clarkson and Watson, 2003; Clarkson et al., 2004; Come et al., 2004; Henson and Tarone, 1994; Jager et al., 1997; Lefebvre et al., 1992; Lyons et al., 2009; McDaniel et al., 2006; O'Brien and Schedin...

Scribner, Kelly C

2013-05-21T23:59:59.000Z

35

Effects of Carbon Nanotubes in Barrier Epithelial Cells via Effects on Lipid Bilayers Shanta Lewis1  

E-Print Network [OSTI]

Effects of Carbon Nanotubes in Barrier Epithelial Cells via Effects on Lipid Bilayers Shanta Lewis1 Physiology, IU School of Medicine Carbon nanotubes (CNTs) are one of many nanoparticles (NP) which are being

Zhou, Yaoqi

36

The plasticity of human breast carcinoma cells is more than epithelial to mesenchymal conversion  

SciTech Connect (OSTI)

The human breast comprises three lineages: the luminal epithelial lineage, the myoepithelial lineage, and the mesenchymal lineage. It has been widely accepted that human breast neoplasia pertains only to the luminal epithelial lineage. In recent years, however, evidence has accumulated that neoplastic breast epithelial cells may be substantially more plastic in their differentiation repertoire than previously anticipated. Thus, along with an increasing availability of markers for the myoepithelial lineage, at least a partial differentiation towards this lineage is being revealed frequently. It has also become clear that conversions towards the mesenchymal lineage actually occur, referred to as epithelial to mesenchymal transitions. Indeed, some of the so-called myofibroblasts surrounding the tumor may indeed have an epithelial origin rather than a mesenchymal origin. Because myoepithelial cells, epithelial to mesenchymal transition-derived cells, genuine stromal cells and myofibroblasts share common markers, we now need to define a more ambitious set of markers to distinguish these cell types in the microenvironment of the tumors. This is necessary because the different microenvironments may confer different clinical outcomes. The aim of this commentary is to describe some of the inherent complexities in defining cellular phenotypes in the microenvironment of breast cancer and to expand wherever possible on the implications for tumor suppression and progression.

Petersen, Ole William; Nielsen, Helga Lind; Gudjonsson, Thorarinn; Villadsen, Ren& #233; Ronnov-Jessen, Lone; Bissell, Mina J.

2001-05-12T23:59:59.000Z

37

The amniote primitive streak is defined by epithelial cell intercalation before gastrulation  

E-Print Network [OSTI]

During gastrulation, a single epithelial cell layer, the ectoderm, generatestwoothers (Gallus gallus) embryos, we reveal a medio-lateral cell intercalation confined to the ectodermal subdomain movements of the mesoderm described in fish and amphibians (anamniotes)4­8 : it occurs before gastrulation

Stern, Claudio

38

Stromal-epithelial interactions in aging and cancer: Senescent fibroblasts alter epithelial cell differentiation  

E-Print Network [OSTI]

Cell. Biol. 18, 4577-4588. DiLeonardo, A. , Linke, S. P. ,Chen et al. , 1995; DiLeonardo et al. , 1994; Krtolica andsenesce by X-irradiation (DiLeonardo et al. , 1994; Robles

Parrinello, Simona; Coppe, Jean-Philippe; Krtolica, Ana; Campisi, Judith

2004-01-01T23:59:59.000Z

39

Epigenetic influences of low-dose bisphenol A in primary human breast epithelial cells  

SciTech Connect (OSTI)

Substantial evidence indicates that exposure to bisphenol A (BPA) during early development may increase breast cancer risk later in life. The changes may persist into puberty and adulthood, suggesting an epigenetic process being imposed in differentiated breast epithelial cells. The molecular mechanisms by which early memory of BPA exposure is imprinted in breast progenitor cells and then passed onto their epithelial progeny are not well understood. The aim of this study was to examine epigenetic changes in breast epithelial cells treated with low-dose BPA. We also investigated the effect of BPA on the ER{alpha} signaling pathway and global gene expression profiles. Compared to control cells, nuclear internalization of ER{alpha} was observed in epithelial cells preexposed to BPA. We identified 170 genes with similar expression changes in response to BPA. Functional analysis confirms that gene suppression was mediated in part through an ER{alpha}-dependent pathway. As a result of exposure to BPA or other estrogen-like chemicals, the expression of lysosomal-associated membrane protein 3 (LAMP3) became epigenetically silenced in breast epithelial cells. Furthermore, increased DNA methylation in the LAMP3 CpG island was this repressive mark preferentially occurred in ER{alpha}-positive breast tumors. These results suggest that the in vitro system developed in our laboratory is a valuable tool for exposure studies of BPA and other xenoestrogens in human cells. Individual and geographical differences may contribute to altered patterns of gene expression and DNA methylation in susceptible loci. Combination of our exposure model with epigenetic analysis and other biochemical assays can give insight into the heritable effect of low-dose BPA in human cells.

Weng, Yu-I; Hsu, Pei-Yin; Liyanarachchi, Sandya; Liu, Joseph; Deatherage, Daniel E.; Huang Yiwen; Zuo Tao; Rodriguez, Benjamin [Human Cancer Genetics Program, Ohio State University, Columbus, OH 43210 (United States); Lin, Ching-Hung; Cheng, Ann-Lii [Department of Internal Medicine and Oncology, National Taiwan University Hospital, Taipei, Taiwan (China); Huang, Tim H.-M., E-mail: Tim.Huang@osumc.ed [Human Cancer Genetics Program, Ohio State University, Columbus, OH 43210 (United States)

2010-10-15T23:59:59.000Z

40

A Role for ZO-1 and PLEKHA7 in Recruiting Paracingulin to Tight and Adherens Junctions of Epithelial Cells*S  

E-Print Network [OSTI]

of Epithelial Cells*S Received for publication,February 14, 2011, and in revised form, March 17, 2011 Published

Halazonetis, Thanos

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Particulate depleted uranium is cytotoxic and clastogenic to human lung epithelial cells  

Science Journals Connector (OSTI)

Depleted uranium (DU) is commonly used in military applications and consequently exposure to soldiers and non-combatants is potentially frequent and widespread. DU is suspected to be a carcinogen, potentially affecting the bronchial cells of the lung. Few studies have considered DU in human bronchial cells. Accordingly, we determined the cytotoxicity and clastogenicity of particulate DU in human bronchial epithelial cells (BEP2D cells). DU-induced concentration-dependent cytotoxicity in human bronchial epithelial cells, and was not clastogenic after 24h but induced chromosomal aberrations after 48h. These data indicate that if DU is a human bronchial carcinogen, it is likely acting through a mechanism that involves DNA breaks after longer exposures.

Carolyne LaCerte; Hong Xie; AbouEl-Makarim Aboueissa; John Pierce Wise Sr.

2010-01-01T23:59:59.000Z

42

Modeling gas phase nitric oxide release in lung epithelial cells Jingjing Jiang a  

E-Print Network [OSTI]

Modeling gas phase nitric oxide release in lung epithelial cells Jingjing Jiang a , Steven C- dated our model with experimental results of gas phase NO release and intracellular L enzyme on NO production. Our model predicts intracellular L-arginine and gas phase NO release over a wide

George, Steven C.

43

Cable-Piliated Burkholderia cepaciaBinds to Cytokeratin 13 of Epithelial Cells  

Science Journals Connector (OSTI)

...ARTICLE MOLECULAR AND CELLULAR PATHOGENESIS Cable-Piliated Burkholderia cepacia Binds to...Canada. This strain expresses surface cable (Cbl) pili and is thought to be the major...epithelial cell (BEC) protein that binds cable pilus-positive B. cepacia. N-terminal...

Umadevi S. Sajjan; Francisco A. Sylvester; Janet F. Forstner

2000-04-01T23:59:59.000Z

44

Regulation of Mammary Tumor Virus Production by Prolactin in BALB/cfC3H Mouse Normal Mammary Epithelial Cells in Vitro  

Science Journals Connector (OSTI)

...prolactin and growth hormone were added to insulin- and hydmocorti sone-containing media, the stimulatory effect did not ex ceed that seen in cultures treated with prolactin, insulin, and hydrocortisone (data not shown). Among the growth hormones tested...

Jason Yang; J. Enami; S. Nandi

1977-10-01T23:59:59.000Z

45

In vitro models for airway epithelial cell culture  

E-Print Network [OSTI]

This work is about the development of a physiologically relevant model of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear ...

Sivathanu, Vivek

2013-01-01T23:59:59.000Z

46

The Staphylococcus aureus Alpha-Toxin Perturbs the Barrier Function in Caco-2 Epithelial Cell Monolayers by Altering Junctional Integrity  

Science Journals Connector (OSTI)

...equipped for epifluorescence with a 75-W xenon arc lamp and a 100 glycerol immersion...Meldolesi J . 1998. Calcium signaling: oscillation, activation, expression. Nature 392...uropathogenic E. coli induces Ca2+ oscillations in renal epithelial cells. Nature 405...

Young-Keun Kwak; Elena Vikstrm; Karl-Eric Magnusson; Beatrix Vcsey-Semjn; Patricia Colque-Navarro; Roland Mllby

2012-02-21T23:59:59.000Z

47

Effect of Energy Restriction on Cell Cycle Machinery in 1-Methyl-1-Nitrosourea-induced Mammary Carcinomas in Rats  

Science Journals Connector (OSTI)

...relationship to the effects of energy restriction on mammary...Thompson H. J. Mechanisms of energy restriction: effects of...McGraw-Hill Publishing Co. New York 1990. 19 Zhu Z., Jiang...Thompson H. J. Effect of energy restriction on the expression...

Weiqin Jiang; Zongjian Zhu; and Henry J. Thompson

2003-03-15T23:59:59.000Z

48

Biological Basis for Radiation Adaptive Responses that Protect Against Bronchial Epithelial Cell Transformation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Basis for Radiation Adaptive Responses that Protect Against Bronchial Basis for Radiation Adaptive Responses that Protect Against Bronchial Epithelial Cell Transformation Wenshu Chen, Xiuling Xu, Lang Bai, Mabel T. Padilla, Carmen Tellez, Katherine M. Gott, Shuguang Leng, Julie A. Wilder, Steven A. Belinsky, Bobby R. Scott and Yong Lin, Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive SE, Albuquerque, NM 87108 The major hypothesis in this project is that low-dose, low linear-energy-transfer (LET) radiation stimulates an adaptive response that protects cells from neoplastic transformation involving modulation of paracrine factors (e.g., cytokines), cell survival/death signaling pathways, and reprogramming of the epigenome. To test this hypothesis, a validated, sensitive in vitro transformation model and a media transfer

49

Mouse mammary tumor virus uses mouse but not human transferrin receptor 1 to reach a low pH compartment and infect cells  

SciTech Connect (OSTI)

Mouse mammary tumor virus (MMTV) is a pH-dependent virus that uses mouse transferrin receptor 1 (TfR1) for entry into cells. Previous studies demonstrated that MMTV could induce pH 5-dependent fusion-from-with of mouse cells. Here we show that the MMTV envelope-mediated cell-cell fusion requires both the entry receptor and low pH (pH 5). Although expression of the MMTV envelope and TfR1 was sufficient to mediate low pH-dependent syncytia formation, virus infection required trafficking to a low pH compartment; infection was independent of cathepsin-mediated proteolysis. Human TfR1 did not support virus infection, although envelope-mediated syncytia formation occurred with human cells after pH 5 treatment and this fusion depended on TfR1 expression. However, although the MMTV envelope bound human TfR1, virus was only internalized and trafficked to a low pH compartment in cells expressing mouse TfR1. Thus, while human TfR1 supported cell-cell fusion, because it was not internalized when bound to MMTV, it did not function as an entry receptor. Our data suggest that MMTV uses TfR1 for all steps of entry: cell attachment, induction of the conformational changes in Env required for membrane fusion and internalization to an appropriate acidic compartment.

Wang Enxiu; Obeng-Adjei, Nyamekye; Ying Qihua [Department of Microbiology and Abramson Family Cancer Center, University of Pennsylvania, 313BRBII/III, 421 Curie Blvd., Philadelphia, PA 19104 (United States); Meertens, Laurent; Dragic, Tanya [Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY (United States); Davey, Robert A. [Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX (United States); Ross, Susan R. [Department of Microbiology and Abramson Family Cancer Center, University of Pennsylvania, 313BRBII/III, 421 Curie Blvd., Philadelphia, PA 19104 (United States)], E-mail: rosss@mail.med.upenn.edu

2008-11-25T23:59:59.000Z

50

Unraveling the microenvironmental influences on the normal mammary gland and induction and progression of breast cancer  

SciTech Connect (OSTI)

The normal mammary gland and invasive breast cancer are both complex 'organs' composed of multiple cell types as well as extracellular matrix (ECM) in three-dimensional (3D) space. Conventionally, both normal and malignant breast cells are studied in vitro as two-dimensional (2D) monolayers of epithelial cells, which results in the loss of structure and tissue function. Many laboratories are now investigating regulation of signaling function in normal mammary gland using 3D cultures. However, it is important also to assay malignant breast cells ex vivo in a physiologically relevant environment to more closely mimic tumor architecture, signal transduction regulation and tumor behavior in vivo. Here we present the potential of these 3D models for drug testing, target validation and guidance of patient selection for clinical trials. We argue also that in order to get full insight into the biology of the normal and malignant breast, and to create in vivo-like models for therapeutic approaches in humans, we need to continue to create more complex heterotypic models to approach the full context the cells encounter in the human body.

Weigelt, Britta; Bissell, Mina J.

2008-06-26T23:59:59.000Z

51

Continuous human cell lines and method of making same  

DOE Patents [OSTI]

Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.

Stampfer, M.R.

1985-07-01T23:59:59.000Z

52

Continuous human cell lines and method of making same  

DOE Patents [OSTI]

Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo[a]pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors.

Stampfer, Martha R. (Oakland, CA)

1989-01-01T23:59:59.000Z

53

Radiation Induced Mammary Cancer  

Science Journals Connector (OSTI)

......Induced Mammary Cancer R.L. Ullrich * R.J. Preston # * Department of Radiation Therapy, University of Texas Medical Branch, Galveston, TX 77550 # Biology Division Oak Ridge National Laboratory Oak Ridge, TN 37831, U.S.A Over the last......

R.L. Ullrich; R.J. Preston

1991-12-01T23:59:59.000Z

54

Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis  

SciTech Connect (OSTI)

Human metapneumovirus (hMPV) is a major cause of lower respiratory tract infections in infants, elderly and immunocompromised patients. Little is known about the response to hMPV infection of airway epithelial cells, which play a pivotal role in initiating and shaping innate and adaptive immune responses. In this study, we analyzed the transcriptional profiles of airway epithelial cells infected with hMPV using high-density oligonucleotide microarrays. Of the 47,400 transcripts and variants represented on the Affimetrix GeneChip Human Genome HG-U133 plus 2 array, 1601 genes were significantly altered following hMPV infection. Altered genes were then assigned to functional categories and mapped to signaling pathways. Many up-regulated genes are involved in the initiation of pro-inflammatory and antiviral immune responses, including chemokines, cytokines, type I interferon and interferon-inducible proteins. Other important functional classes up-regulated by hMPV infection include cellular signaling, gene transcription and apoptosis. Notably, genes associated with antioxidant and membrane transport activity, several metabolic pathways and cell proliferation were down-regulated in response to hMPV infection. Real-time PCR and Western blot assays were used to confirm the expression of genes related to several of these functional groups. The overall result of this study provides novel information on host gene expression upon infection with hMPV and also serves as a foundation for future investigations of genes and pathways involved in the pathogenesis of this important viral infection. Furthermore, it can facilitate a comparative analysis of other paramyxoviral infections to determine the transcriptional changes that are conserved versus the one that are specific to individual pathogens.

Bao, X. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States); Sinha, M. [Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas (United States)]|[UTMB Bioinformatics Program, University of Texas Medical Branch, Galveston, Texas (United States); Liu, T.; Hong, C. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States); Luxon, B.A. [Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas (United States)]|[UTMB Bioinformatics Program, University of Texas Medical Branch, Galveston, Texas (United States); Garofalo, R.P. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States)]|[Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas (United States)]|[Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas (United States); Casola, A. [Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas (United States)]|[Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas (United States)]|[Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas (United States)], E-mail: ancasola@utmb.edu

2008-04-25T23:59:59.000Z

55

Cell-cycle dependent localization of MELK and its new partner RACK1 in epithelial versus mesenchyme-like  

E-Print Network [OSTI]

Cell-cycle dependent localization of MELK and its new partner RACK1 in epithelial versus mesenchyme. Receptor for Activated protein Kinase C (RACK1), which we identified as an xMELK partner, co-localizes with xMELK at the tight junction. Moreover, a truncated RACK1 construct interferes with i

Paris-Sud XI, Université de

56

3624 Biophysical Journal Volume 84 June 2003 36243635 Discrete Models of Autocrine Cell Communication in Epithelial Layers  

E-Print Network [OSTI]

of Chemical Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton-to- cell communication produces spatially nonuniform patterns in the expression of genes that guide the development of tissues and organs. The design principles of epithelial patterning are being formulated only

Shvartsman, Stanislav "Stas"

57

Differential expression of MHC class II antigens and cathepsin L by subtypes of cortical epithelial cells in the rat thymus: an immunoelectron microscopic study  

Science Journals Connector (OSTI)

......3 de Waal E J and Rademakers L H P M (1997) Heterogeneity of epithelial cells in the...40 van de Wijngaert F P, Kendall M D, Schuurman H J, Rademakers L H P M, and Kater L (1984) Heterogeneity of epithelial......

Yamini Arudchelvan; Yukio Nishimura; Nobuko Tokuda; Tomoo Sawada; Fumihiko Shinozaki; Tetsuo Fukumoto

2002-05-01T23:59:59.000Z

58

E-Print Network 3.0 - alveolar epithelial type Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cells. Alveolar epithelial cells adherence to type I collagen was decreased... M CaCl2. Cell cultures Isolation and primary culture of alveolar epithelial cells Alveolar...

59

Antigen presentation by small intestinal epithelial cells uniquely enhances IFN-? secretion from CD4{sup +} intestinal intraepithelial lymphocytes  

SciTech Connect (OSTI)

Highlights: Small intestinal epithelial cells (sIECs). sIECs are able to induce antigen specific proliferation of CD4{sup +} IELs. sIECs induce markedly enhanced IFN-? secretion by CD4{sup +} IELs. Induction of enhanced IFN-? secretion by sIECs is uniquely observed in CD4{sup +} IELs. -- Abstract: Small intestinal epithelial cells (sIECs) express major histocompatibility complex class II molecules even in a normal condition, and are known to function as antigen presenting cells (APCs) at least in vitro. These findings raised the possibility that sIECs play an important role in inducing immune responses against luminal antigens, especially those of intestinal intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs). We herein showed that antigenic stimulation with sIECs induced markedly greater secretion of interferon-gamma (IFN-?) by CD4{sup +} IELs, but not interleukin (IL)-4, IL-10 and IL-17 although the proliferative response was prominently lower than that with T cell-depleted splenic APCs. In contrast, no enhanced IFN-? secretion by CD4{sup +} LPLs and primed splenic CD4{sup +} T cells was observed when stimulated with sIECs. Taken together, these results suggest that sIECs uniquely activate CD4{sup +} IELs and induce remarkable IFN-? secretion upon antigenic stimulation in vivo.

Hatano, Ryo; Yamada, Kiyoshi; Iwamoto, Taku; Maeda, Nana; Emoto, Tetsuro; Shimizu, Makoto; Totsuka, Mamoru, E-mail: atotuka@mail.ecc.u-tokyo.ac.jp

2013-06-14T23:59:59.000Z

60

Sustained activation of STAT5 is essential for chromatin remodeling and maintenance of mammary-specific function  

SciTech Connect (OSTI)

Epithelial cells, once dissociated and placed in two-dimensional (2D) cultures, rapidly lose tissue-specific functions. We showed previously that in addition to prolactin, signaling by laminin-111 was necessary to restore functional differentiation of mammary epithelia. Here, we elucidate two additional aspects of laminin-111 action. We show that in 2D cultures, the prolactin receptor is basolaterally localized and physically segregated from its apically placed ligand. Detachment of the cells exposes the receptor to ligation by prolactin leading to signal transducers and activators of transcription protein 5 (STAT5) activation, but only transiently and not sufficiently for induction of milk protein expression. We show that laminin-111 reorganizes mammary cells into polarized acini, allowing both the exposure of the prolactin receptor and sustained activation of STAT5. The use of constitutively active STAT5 constructs showed that the latter is necessary and sufficient for chromatin reorganization and {beta}-casein transcription. These results underscore the crucial role of continuous laminin signaling and polarized tissue architecture in maintenance of transcription factor activation, chromatin organization, and tissue-specific gene expression.

Xu, Ren; Nelson, Celeste M.; Muschler, John L.; Veiseh, Mandana; Vonderhaar, Barbara K.; Bissell, Mina J.

2009-06-03T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Oncogene-Mediated Human Lung Epithelial Cell Transformation Produces Adenocarcinoma Phenotypes In Vivo  

Science Journals Connector (OSTI)

...It has been challenging to engineer lung adenocarcinoma models...challenges facing the field is to engineer transformed lung epithelial...at 37C under a low oxygen environment (3% O2 and 5% CO2) in...It has been challenging to engineer lung adenocarcinoma models...

Ken Sasai; Taiko Sukezane; Emmy Yanagita; Harumi Nakagawa; Azusa Hotta; Tomoo Itoh; and Tsuyoshi Akagi

2011-04-01T23:59:59.000Z

62

A link between stem cells and the EMT phenotype induced by IR and TGFβ  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

link between stem cells and the EMT phenotype induced by IR and TGFβ link between stem cells and the EMT phenotype induced by IR and TGFβ Ignacio Fernandez-Garcia New York University School of Medicine Abstract Epithelial-Mesenchymal Transition (EMT) occurs as key developmental program but is also often activated during cancer progression. EMT is characterized by loss of epithelial cell polarity, loss of cell-cell contacts, and acquisition of mesenchymal markers and phenotypic traits that include increased cell motility1. Approximately 18% of breast cancers exhibit evidence of EMT. We have shown that the progeny of irradiated human mammary epithelial cells (HMEC) undergo EMT when exposed to transforming growth factor β1 (TGFβ). Additionally, recent publication from Weinberg and colleagues showed that induction of EMT through engineered expression of

63

Chronic occupational exposure to arsenic induces carcinogenic gene signaling networks and neoplastic transformation in human lung epithelial cells  

SciTech Connect (OSTI)

Chronic arsenic exposure remains a human health risk; however a clear mode of action to understand gene signaling-driven arsenic carcinogenesis is currently lacking. This study chronically exposed human lung epithelial BEAS-2B cells to low-dose arsenic trioxide to elucidate cancer promoting gene signaling networks associated with arsenic-transformed (B-As) cells. Following a 6 month exposure, exposed cells were assessed for enhanced cell proliferation, colony formation, invasion ability and in vivo tumor formation compared to control cell lines. Collected mRNA was subjected to whole genome expression microarray profiling followed by in silico Ingenuity Pathway Analysis (IPA) to identify lung carcinogenesis modes of action. B-As cells displayed significant increases in proliferation, colony formation and invasion ability compared to BEAS-2B cells. B-As injections into nude mice resulted in development of primary and secondary metastatic tumors. Arsenic exposure resulted in widespread up-regulation of genes associated with mitochondrial metabolism and increased reactive oxygen species protection suggesting mitochondrial dysfunction. Carcinogenic initiation via reactive oxygen species and epigenetic mechanisms was further supported by altered DNA repair, histone, and ROS-sensitive signaling. NF-?B, MAPK and NCOR1 signaling disrupted PPAR?/?-mediated lipid homeostasis. A pro-cancer gene signaling network identified increased survival, proliferation, inflammation, metabolism, anti-apoptosis and mobility signaling. IPA-ranked signaling networks identified altered p21, EF1?, Akt, MAPK, and NF-?B signaling networks promoting genetic disorder, altered cell cycle, cancer and changes in nucleic acid and energy metabolism. In conclusion, transformed B-As cells with their whole genome expression profile provide an in vitro arsenic model for future lung cancer signaling research and data for chronic arsenic exposure risk assessment. Highlights: ? Chronic As{sub 2}O{sub 3} exposure to lung epithelial cells resulted in a cancer-like phenotype. ? Mice injected with arsenic transformed (B-As) cells displayed metastatic tumors. ? Microarray profiling revealed changes in mitochondrial metabolism and ROS response. ? p21, EF1?, Akt, MAPK, PPAR? and NF-?B networks promoted pro-cancer signaling. ? B-As cells represent a lung cancer model to explore As-associated carcinogenesis.

Stueckle, Todd A., E-mail: tstueckle@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Lu, Yongju, E-mail: yongju6@hotmail.com [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Davis, Mary E., E-mail: mdavis@wvu.edu [Department of Physiology, West Virginia University, Morgantown, WV 26506 (United States); Wang, Liying, E-mail: lmw6@cdc.gov [Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States)] [Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Jiang, Bing-Hua, E-mail: bhjiang@jefferson.edu [Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)] [Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Holaskova, Ida, E-mail: iholaskova@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Schafer, Rosana, E-mail: rschafer@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Barnett, John B., E-mail: jbarnett@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Rojanasakul, Yon, E-mail: yrojan@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)

2012-06-01T23:59:59.000Z

64

Inhibition by Parvovirus H-1 of the Formation of Tumors in Nude Mice and Colonies in Vitro by Transformed Human Mammary Epithelial Cells  

Science Journals Connector (OSTI)

...Melero was also funded by the Departamento de Educacion del Gobierno de Navarra, Departamento de Salud del Gobierno de Navarra, Redes tematicas de investigacion cooperativa RETIC (RD06/0020/0065), European commission VII framework program (ENCITE...

Thierry Dupressoir; Jean-Marc Vanacker; Jan J. Cornelis; Nadine Duponchel; Jean Rommelaere

1989-06-15T23:59:59.000Z

65

Morphologically Normal-Appearing Mammary Epithelial Cells Obtained from High-Risk Women Exhibit Methylation Silencing of INK4a/ARF  

Science Journals Connector (OSTI)

...CA58207 (T.D. Tlsty). The costs of publication of this article...Review Board at Duke University Medical Center, in accordance with...were used under reduced lighting and stored in a desiccator...initial RPFNA at Duke University Medical Center from March 2003 to August...

Gregory R. Bean; Andrew D. Bryson; Patrick G. Pilie; Vanessa Goldenberg; Joseph C. Baker, Jr.; Catherine Ibarra; Danielle M.U. Brander; Carolyn Paisie; Natalie R. Case; Mona Gauthier; Paul A. Reynolds; Eric Dietze; Julie Ostrander; Victoria Scott; Lee G. Wilke; Lisa Yee; Bruce F. Kimler; Carol J. Fabian; Carola M. Zalles; Gloria Broadwater; Thea D. Tlsty; and Victoria L. Seewaldt

2007-11-15T23:59:59.000Z

66

Gene expression analysis of human primary prostate epithelial and fibroblast cell cultures to an acute dose of 10cGy  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

26, 2011 26, 2011 Gene expression analysis of human primary prostate epithelial and fibroblast cell cultures to an acute dose of 10cGy J. Tyson McDonald, Julia Fox, Heather Szelag, Annie Kang, Heiko Enderling, Peter Nowd, Douglas Scheinder, Giannoula Lakka Klement, Ingolf Tuerk, and Lynn Hlatky Center of Cancer Systems Biology, Steward St. Elizabeth's Medical Center, 736 Cambridge Street, Boston, Massachusetts 02135. Primary tissue represents a better model for studies than immortalized cell lines that are adapted

67

TAK1 regulates NF-{Kappa}B and AP-1 activation in airway epithelial cells following RSV infection  

SciTech Connect (OSTI)

Respiratory syncytial virus (RSV) is the most common cause of epidemic respiratory diseases in infants and young children. RSV infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including Nuclear Factor-{kappa}B (NF-{kappa}B) and AP-1. In this study, we have investigated the signaling pathway leading to activation of these two transcription factors in response to RSV infection. Our results show that IKK{beta} plays a key role in viral-induced NF-{kappa}B activation, while JNK regulates AP-1-dependent gene transcription, as demonstrated by using kinase inactive proteins and chemical inhibitors of the two kinases. Inhibition of TAK1 activation, by overexpression of kinase inactive TAK1 or using cells lacking TAK1 expression, significantly reduced RSV-induced NF-{kappa}B and AP-1 nuclear translocation and DNA-binding activity, as well as NF-{kappa}B-dependent gene expression, identifying TAK1 as an important upstream signaling molecule regulating RSV-induced NF-{kappa}B and AP-1 activation. - Highlights: > IKK{beta} is a major kinase involved in RSV-induced NF-{kappa}B activation. > JNK regulates AP-1-dependent gene transcription in RSV infection. > TAK1 is a critical upstream signaling molecule for both pathways in infected cells.

Dey, Nilay; Liu Tianshuang [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Garofalo, Roberto P. [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX (United States); Department of Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX (United States); Casola, Antonella, E-mail: ancasola@utmb.edu [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX (United States); Department of Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX (United States)

2011-09-30T23:59:59.000Z

68

A Role for the Integrin ?v?8 in the Negative Regulation of Epithelial Cell Growth  

Science Journals Connector (OSTI)

...to I) agreed with the conservation of this amino acid in...5% CO2 in humidified water-jacketed CO2 incubators...G418 (1 mg/ml). Pools of b8-expressing cells...Becton Dickinson). Pools of b8, b6, and b6...independently transduced pools of cells. SW480 cells...

Stephanie Cambier; De-zhi Mu; David OConnell; Kevin Boylen; William Travis; Wei-hong Liu; V. Courtney Broaddus; and Stephen L. Nishimura

2000-12-15T23:59:59.000Z

69

Toll like receptor-3 priming alters diesel exhaust particle-induced cytokine responses in human bronchial epithelial cells  

Science Journals Connector (OSTI)

Abstract Inflammation is considered central in the pathology of health effects from airborne particulate matter (PM). Preexisting inflammatory disorders, such as asthma, but also pulmonary infections, appear to be a risk factor of adverse health effects from PM exposure. Thus, to assess whether and how preexisting inflammation may sensitize lung cells toward additional proinflammatory effects of PM, human bronchial epithelial cells (BEAS-2B) were primed with the highly proinflammatory Toll-like receptor 3 (TLR3) ligand, Poly I:C, prior to exposure with diesel exhaust particles (DEP). DEP-exposure alone induced increased gene-expression of interleukin-6 (IL-6) and CXCL8 (IL-8) but did not affect expression of CCL5 (RANTES), while TLR3-priming alone induced expression of IL-6, CXCL8 and CCL5. DEP-exposure exacerbated IL-6 and CXCL8 responses in TLR3-primed cells, while TLR3-induced CCL5 was suppressed by DEP. TLR3-priming and DEP-exposure resulted in possible additive effects on p38 phosphorylation and I?B-degradation, while DEP rather suppressed ERK and JNK-activation. However, TLR3-priming elicited a considerable increase in p65-phosphorylation at serine 536 which is known to enhance the transcriptional activity of NF-?B. DEP-exposure was unable to induce p65-phosphorylation. Thus TLR3-priming may affect susceptibility toward DEP by activating both shared and complementing pathways required for optimal expression of proinflammatory genes such as IL-6 and CXCL8. The study underscores that primed sick cells may be more susceptible toward effects of particle-exposure and respond both stronger and differently compared to unprimed healthy cells.

Nicolai S. Bach; Marit Lg; Johan vrevik

2014-01-01T23:59:59.000Z

70

miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohns disease  

SciTech Connect (OSTI)

Highlights: NOD2 is a target gene of miR-122. miR-122 inhibits LPS-induced apoptosis by suppressing NOD2 in HT-29 cells. miR-122 reduces the expression of pro-inflammatory cytokines (TNF-? and IFN-?). miR-122 promotes the release of anti-inflammatory cytokines (IL-4 and IL-10). NF-?B signaling pathway is involved in inflammatory response induced by LPS. -- Abstract: Crohns disease (CD) is one of the two major types of inflammatory bowel disease (IBD) thought to be caused by genetic and environmental factors. Recently, miR-122 was found to be deregulated in association with CD progression. However, the underlying molecular mechanisms remain unclear. In the present study, the gene nucleotide-binding oligomerization domain 2 (NOD2/CARD15), which is strongly associated with susceptibility to CD, was identified as a functional target of miR-122. MiR-122 inhibited LPS-induced apoptosis by suppressing NOD2 in HT-29 cells. NOD2 interaction with LPS initiates signal transduction mechanisms resulting in the activation of nuclear factor ?B (NF-?B) and the stimulation of downstream pro-inflammatory events. The activation of NF-?B was inhibited in LPS-stimulated HT-29 cells pretreated with miR-122 precursor or NOD2 shRNA. The expression of the pro-inflammatory cytokines TNF-? and IFN-? was significantly decreased, whereas therelease of the anti-inflammatory cytokines IL-4 and IL-10 was increased in LPS-stimulated HT-29 cells pretreated with miR-122 precursor, NOD2 shRNA or the NF-?B inhibitor QNZ. Taken together, these results indicate that miR-122 and its target gene NOD2 may play an important role in the injury of intestinal epithelial cells induced by LPS.

Chen, Yu; Wang, Chengxiao; Liu, Ying; Tang, Liwei; Zheng, Mingxia [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China); Xu, Chundi [Department of Pediatrics, Ruijin affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200025 (China)] [Department of Pediatrics, Ruijin affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200025 (China); Song, Jian, E-mail: jiansongkxy@126.com [Department of Gastroenterology, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Gastroenterology, Jiangwan Hospital of Shanghai, Shanghai 200434 (China); Meng, Xiaochun [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)

2013-08-16T23:59:59.000Z

71

Investigation of non-targeted effects of low dose ionizing radiation on the mammary gland  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

non-targeted effects of low dose ionizing radiation on the mammary gland non-targeted effects of low dose ionizing radiation on the mammary gland utilizing three-dimensional culture models of mammary cells derived from mouse strains that differ in susceptibility to tumorigenesis Joni D. Mott, Antoine M. Snijders, Alvin Lo, Dinah Levy-Groesser, Bahram Parvin, Andrew J. Wyrobek, Jian-Hua Mao, and Mina J. Bissell Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley CA 94720 Goal: Within the Lawrence Berkeley National Laboratory's SFA, Project 2, our studies focus on utilizing three dimensional (3D) cell culture models as surrogates for in vivo studies to determine how low doses of ionizing radiation influence mammary gland tissue architecture and how this may relate both to tumor progression and/or adaptive response.

72

Using Three Dimensional Cell Culture and Tissue Architecture to Monitor an  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Three Dimensional Cell Culture and Tissue Architecture to Monitor an Three Dimensional Cell Culture and Tissue Architecture to Monitor an Adaptive Response in Mammary Epithelial Cells Mina Bissell Lawrence Berkeley National Laboratory Abstract Exposure of tissues to ionizing radiation results in targeted effect on cells as well as non-targeted effects on tissues. Although, targeted effects such as the DNA damage response have been studied extensively, non-targeted effects leading to modification in tissue architecture and tumor progression have been less studied and are not well understood. The mammary gland is a tissue that has been shown to be susceptible to tumor formation and cancer progression following exposure to ionizing radiation. In conjunction with the laboratories of Mary Helen Barcellos-Hoff and Catherine Park we showed previously that in the presence of TGF-β,

73

Classification of Human Epithelial Type 2 Cell Indirect Immunofluoresence Images via Codebook Based Descriptors  

E-Print Network [OSTI]

, Australia Sullivan Nicolaides Pathology, Australia Abstract The Anti-Nuclear Antibody (ANA) clinical of several vari- ants of the descriptor on two publicly available datasets: ICPR HEp-2 cell classification contest dataset and the new SNPHEp-2 dataset. To our knowledge, this is the first time codebook

Sanderson, Conrad

74

Ionizing Radiation Induces a Transient Increase in Cytosolic Free [Ca2+] in Human Epithelial Tumor Cells  

Science Journals Connector (OSTI)

...MDA-MB-231 and HT-29 cells, oscillations in cytosolic [Ca2i@ levels...microscope was used with a 75 W Xenon arc, Nikon UVF 40X (numerical...mm was observed as well as oscillations with a period of 2.8 0...the responders by multiple oscillations in [Ca2@] . . . . . As...

Daniel G. Todd and Ross B. Mikkelsen

1994-10-01T23:59:59.000Z

75

Endostatin regulates branching morphogenesis of renal epithelial cells and ureteric bud  

Science Journals Connector (OSTI)

...Nature (London) 400 : 281 284 , 10421372 . 38 Tsuda M Kamimura K Nakato H Archer M Staatz W Fox B Humphrey M Olson S Futch T Kaluza V ( 1999 ) Nature (London) 400 : 276 280 , 10421371 . 39 Lander A D Selleck S B ( 2000 ) J Cell Biol 148 : 227...

Anil Karihaloo; S. Ananth Karumanchi; Jonathan Barasch; Vivekanand Jha; Christian Hans Nickel; Jun Yang; Silviu Grisaru; Kevin T. Bush; Sanjay Nigam; Norman D. Rosenblum; Vikas P. Sukhatme; Lloyd G. Cantley

2001-01-01T23:59:59.000Z

76

Changes in Stem Cell Populations of Rat Tracheal Epithelial Cell Cultures at an Early Stage in Neoplastic Progression  

Science Journals Connector (OSTI)

...cells to form colonies after 7 days with a CFE of 2 to 10%. Removal of the feeder cells...trach alepithelial; EG, enhanced growth; CFE, colony-forming efficiency; CFU, colony-forming...formed colonies within 5 to 7 days, with a CFE of 2 to 10%. The morphology and size of...

David Thomassen; J. Carl Barrett; Diane K. Beeman; Paul Nettesheim

1985-07-01T23:59:59.000Z

77

Detection of Ductal Dysplasia in Mammary Outgrowths Derived from Carcinogen-treated Virgin Female BALB/c Mice  

Science Journals Connector (OSTI)

...National Laboratory [R. L. U.], Oak Ridge, Tennessee 37830 ABSTRACT These...mammary cells. In addition, several workers have reported that expression of...studied by Medina (17). These workers have performed serial transplan tation...

Stephen P. Ethier and Robert L. Ullrich

1982-05-01T23:59:59.000Z

78

Effect of Preirradiation of the Tumor Bed on the Relative Vascular Space of Mouse Gastric Adenocarcinoma 328 and Mammary Adenocarcinoma CA755  

Science Journals Connector (OSTI)

...viable portions of tumors of the MTG-B strain show an accelerating...not reflect the fundamental processes occurring in those areas of...Growth and Cell Loss in the MTG-B Mouse Mammary Carcinoma...of Mouse Mammary Tumor Strain MTG-B. Proc. Soc. Exptl. Biol...

Randy Jirtle and Kelly H. Clifton

1973-04-01T23:59:59.000Z

79

Berthon P, Katoh M, Dusanter-Fourt 1, Kelly PA, Djiane J, 1986b. Purification of prolactin receptor from sow mam-mary gland and polyclonal antibodies production. Mol Cell Endocrinol, soumis publication  

E-Print Network [OSTI]

Berthon P, Katoh M, Dusanter-Fourt 1, Kelly PA, Djiane J, 1986b. Purification of prolactin receptor publication Djiane J, Durand P, Kelly PA, 1977. Evolution of prolactin receptors in rabbit mammary gland during pregnancy and lactation. Endocrinology, 100:1348-1356 Djiane J, Dusanter-Fourt 1, Katoh M, Kelly

Paris-Sud XI, Université de

80

Neoplastic Transformation of a Human Colonic Epithelial Cell Line: In Vitro Evidence for the Adenoma to Carcinoma Sequence  

Science Journals Connector (OSTI)

...AA/C1 [colony-forming efficiency (CFE) on plastic of 1.05%] was isolated...C1/SB cell line which had an increased CFE on plastic (6.13%) although the cells...On continuous in vitro passage, the CFE in agarose of AA/C1/SB10 has increased...

Ann C. Williams; Sara J. Harper; Christos Paraskeva

1990-08-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

The recruitment of stromal cells to the site of tumor formation  

E-Print Network [OSTI]

Myofibroblasts are an alpha-smooth muscle actin ([alpha]-SMA)-expressing cell type found within human mammary carcinomas, but not in the normal mammary gland. Myofibroblasts can enhance tumor formation by promoting ...

Saelzler, Matthew P. (Matthew Paul)

2010-01-01T23:59:59.000Z

82

E-Print Network 3.0 - a549 lung epithelial Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

PROLIFERATION AND PROTEIN EXPRESSION OF LUNG CANCER CELLS Summary: (NSCLC) cell lines. Methods: The epithelial lung carcinoma (A549 ) and a bronchioalveolar (NCI-H358......

83

Isolation of Two Distinct Epithelial Cell Lines from a Single Feline Mammary Carcinoma with Different Tumorigenic Potential in Nude Mice and Expressing Different Levels of Epidermal Growth Factor Receptors  

Science Journals Connector (OSTI)

...vitro to the in vivo situation. A more fundamental question is whether abnormal expres sion...Jones, and G. Migaki (eds.). Handbook Animal Models of Human Disease, Fascini...Comparative Pathology, Washington, DC: Armed Forces Institute of Pathology, 1987. 4. Stolwijk...

Jules M. H. M. Minke; Ed Schuuring; Raymond van den Berghe; John A. M. Stolwijk; Johannes Boonstra; Cees Cornelisse; John Hilkens; and Wim Misdorp

1991-08-01T23:59:59.000Z

84

The int-2 gene product acts as a growth factor and substitutes for basic fibroblast growth factor in promoting the differentiation of a normal mouse mammary epithelial cell line  

Science Journals Connector (OSTI)

...timepoint, one biopsy is depicted as a single pie chart at each 2-cm interval from the gastroesophageal junction (GEJ) with each pie chart showing the distribution of SCA types in each biopsy. The size of each pie chart is representational of different amounts...

T Venesio; D Taverna; NE Hynes; R Deed; D MacAllan; F Ciardiello; EM Valverius; DS Salomon; R Callahan; G Merlo

1992-01-01T23:59:59.000Z

85

Abstract 1437: PTEN deficiency in prostate epithelial cells is associated with increased CAD phosphorylation and is inhibited by fisetin  

Science Journals Connector (OSTI)

...Abstract 3831: The dietary flavonoid fisetin negatively regulates NEDD9 expression...previously showed that the dietary flavonoid fisetin disrupted Wnt/beta-catenin signaling...including melanoma. Here, we report that fisetin treatment of melanoma cells resulted in...

Mohammad Imran Khan; Vaqar Mustafa Adhami; Omar Mohammad Haidar; Bilal Bin Hafeez; Ajit Kumar Verma; Hasan Mukhtar

2014-10-01T23:59:59.000Z

86

Sensitivity of Hamster Tracheal Epithelial Cells to Asbestiform Minerals Modulated by Serum and by Transforming Growth Factor ?  

Science Journals Connector (OSTI)

...magnesium-free phosphate buffered saline; CFE, colony forming efficiency; NHBE, normal...the duration of the assay(s). Assay of CFE. Responses to agents were measured using...number of cells plated was calculated as the CFE. Results, expressed as the relative survival...

Ann Marie Sesko and Brooke T. Mossman

1989-05-15T23:59:59.000Z

87

Growth Characteristics and Enzyme Activities in a Survey of Transformation Markers in Adult Rat Liver Epithelial-like Cell Cultures  

Science Journals Connector (OSTI)

...tumorigenic ity and growth in soft agar. CFE's in 10 and 1% serum media, although...A; GGT, y-glutamyl transpeptidase: CFE. colony-forming efficiency; PA. plasmin...nation and for cell line establishment. CFE in Soft Agar. All the lines were tested...

Richard H. C. San; Tomiko Shimada; Carol J. Maslansky; Dawn M. Kreiser; Michael F. Laspia; Jerry M. Rice; Gary M. Williams

1979-11-01T23:59:59.000Z

88

Abstract A20: Deacetylation of histone H3 at lysine 9 with ethanol in human colonic epithelial cells  

Science Journals Connector (OSTI)

...Modulation of PPARalpha and PPARbeta by ethanol in human breast cancer cell lines Nagaraj...14-18, 2007; Los Angeles, CA 3464 Ethanol consumption is associated with an increased...mechanisms involved are still unclear. Ethanol modulates isoforms of the peroxisome proliferator-activated...

Hyeran Jang; Mary Moyer; Sang-Woon Choi

2008-11-01T23:59:59.000Z

89

Transcription of the Tollip gene is elevated in intestinal epithelial cells through impaired O-GlcNAcylation-dependent nuclear translocation of the negative regulator Elf-1  

SciTech Connect (OSTI)

Highlights: {yields} Transcriptional activation of the Tollitip gene is higher in IECs than in monocytes. {yields} Nt -194/-186 region acts as a cis-element and is recognized by Elf-1. {yields} Elf-1 suppresses Tollip gene transcription in monocytes but not in IECs. {yields} O-GlcNAc modification is necessary for nuclear translocation of Elf-1. {yields} O-GlcNAcylation-dependent nuclear translocation of Elf-1 is impaired in IECs. -- Abstract: Intestinal epithelial cells (IECs) must be tolerant of the large number of commensal bacteria inhabiting the intestinal tract to avoid excessive inflammatory reactions. Toll-interacting protein (Tollip), a negative regulator of Toll-like receptor signaling, is known to be expressed at high levels in IECs, and to thereby contribute to the hyporesponsiveness of IECs to commensals. In this study, we analyzed the underlying mechanisms for elevated transcription of the Tollip gene in IECs using a human IEC line, Caco-2, and a human monocyte line, THP-1, as a control. Elf-1 was identified as a transcription factor that negatively regulates Tollip gene expression. The transcription factor Elf-1 was localized in the nucleus by O-linked N-acetylglucosamine (O-GlcNAc) modification, whereas the unmodified form was detected only in the cytoplasm. Comparison of Caco-2 and THP-1 cells revealed that O-GlcNAc modification of Elf-1 was significantly lower in IECs than in monocytes. Collectively, the results indicate that insufficient O-GlcNAc modification prevents Elf-1-mediated transcriptional repression and thereby upregulates Tollip gene expression in IECs.

Sugi, Yutaka [College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880 (Japan)] [College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880 (Japan); Takahashi, Kyoko, E-mail: ktaka@brs.nihon-u.ac.jp [College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880 (Japan)] [College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880 (Japan); Nakano, Kou; Hosono, Akira; Kaminogawa, Shuichi [College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880 (Japan)] [College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880 (Japan)

2011-09-09T23:59:59.000Z

90

Intracellular trafficking and plasma membrane microdomain distribution of the NSP4 enterotoxin during rotavirus infection in epithelial cells  

E-Print Network [OSTI]

Sterol Transfer from Detergent-Resistant Membranes (DRM) and Detergent-Soluble Membranes (DSM) ????????????? 31 5 Sterol Transfer from Non-detergent Caveolae/Rafts ???.?............. 34 6 Sterol Transfer from Affinity-purified Caveolae... pups and pup intestinal epithelia induced diarrhea and fluid accumulation with chloride secretion, respectively (Ball et al., 1996). Addition of purified NSP4 to HT-29 cells induces a rapid rise in phospholipase C (PLC)-catalyzed inositiol 1...

Storey, Stephen Michael

2009-05-15T23:59:59.000Z

91

Antioxidant and Anti-Inflamatory Effects and Mechanisms of Green Tea in Vitro in Vascular Epithelial Cells  

E-Print Network [OSTI]

or lone electrons. These electrons are reactive and have the capability to cause damage to tissues if left in their unstable state. In order to counteract these free radicals there are antioxidants which seek out the free radicals and bind to them... Cells. (April 2009) Abida Hasan Department of Nutritional Sciences Texas A&M University Research Advisor: Dr. Susanne Talcott Department of Nutritional Sciences In the human body there are free radicals present which have one or many unpaired...

Hasan, Abida

2011-08-04T23:59:59.000Z

92

Detection of Human Lung Epithelial Cell Growth Factors Produced by a Lung Carcinoma Cell Line: Use in Culture of Primary Solid Lung Tumors  

Science Journals Connector (OSTI)

...PMSF, phenylmethylsulfonylfluoride; CFE, colony-forming efficiency. of cells...cells to A549-I CM. Fold increase in CFE over controls is shown for the response...responsedeterminedas percentageof control CFE. Fivewellswereusedper conditionand the...

Jill M. Siegfried

1987-06-01T23:59:59.000Z

93

Neutron-induced Mammary Neoplasms in the Rat  

Science Journals Connector (OSTI)

...Research, Inc. May 1972 research-article Neutron-induced Mammary Neoplasms in the Rat...Medicine, Memphis, Tennessee 38103 Fission neutrons are effective in producing mammary neoplasms...does not hold after exposure to fission neutrons, at least from 10 to 250 rads, in single...

Howard H. Vogel, Jr. and Robert Zaldvar

1972-05-01T23:59:59.000Z

94

Inhibition of ICAM-1/LFA-1-mediated Heterotypic T-cell Adhesion to Epithelial Cells: Design of ICAM-1 Cyclic Peptides  

E-Print Network [OSTI]

In this work, we have designed cyclic peptides (cIBL, cIBR, cIBC, CH4 and CH7) derived from the parent IB peptide (ICAM-1121) that are inhibitors of ICAM-1/LFA-1-mediated T-cell adhesion to Caco-2 cell monolayers. Cyclic ...

Anderson, Meagan E.; Yakovleva, Tatyana; Yongbo, Hu; Siahaan, Teruna J.

2004-03-01T23:59:59.000Z

95

Had-1, a Uridine 5?-Diphosphogalactose Transport-defective Mutant of Mouse Mammary Tumor Cell FM3A: Composition of Glycolipids, Cell Growth Inhibition by Lactosylceramide, and Loss of Tumorigenicity  

Science Journals Connector (OSTI)

...Lee 8, which was charac terized to be defective in the UDP-galactose transport system...converted to TMS derivatives. Compositions of car bohydrates, fatty acids, and long chain...and the mutant cell line was found to be defective in UDP-galactose transport to Golgi...

Takao Taki; Kiyoshi Ogura; Chiaki Rokukawa; Takahiko Hara; Masao Kawakita; Tamao Endo; Akira Kobata; and Shizuo Handa

1991-03-15T23:59:59.000Z

96

E-Print Network 3.0 - advanced ovarian epithelial Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

as an animal model of human epithelial ovarian cancer (15, 26). ... Source: Mayo, Kelly E. - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern...

97

E-Print Network 3.0 - advanced epithelial ovarian Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

as an animal model of human epithelial ovarian cancer (15, 26). ... Source: Mayo, Kelly E. - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern...

98

E-Print Network 3.0 - activation disrupts epithelial Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

185-194 Plasticity of epithelial cell shape in response... expresses enhanced green fluorescent protein (eGFP) specifically in the ectodermal ... Source: Bosch, Thomas C. G....

99

Phenotypic Characteristics of Cell Lines Derived from Disseminated Cancer Cells in Bone Marrow of Patients with Solid Epithelial Tumors: Establishment of Working Models for Human Micrometastases  

Science Journals Connector (OSTI)

...serum deprivation. Exp. Cell Res., 224: 208-213, 1996. 59 Van der Velde-Zimmermann D., Verdaasdonk M. A., Rademakers L. H., De Weger R. A., Van den Tweel J. G., Jogling P. Fibronectin distribution in human bone marrow stroma: matrix...

Elmar Putz; Klaus Witter; Sonja Offner; Peter Stosiek; Alfred Zippelius; Judith Johnson; Robert Zahn; Gert Riethmller; Klaus Pantel

1999-01-01T23:59:59.000Z

100

Extracellular matrix control of mammary gland morphogenesis and tumorigenesis: insights from imaging  

SciTech Connect (OSTI)

The extracellular matrix (ECM), once thought to solely provide physical support to a tissue, is a key component of a cell's microenvironment responsible for directing cell fate and maintaining tissue specificity. It stands to reason, then, that changes in the ECM itself or in how signals from the ECM are presented to or interpreted by cells can disrupt tissue organization; the latter is a necessary step for malignant progression. In this review, we elaborate on this concept using the mammary gland as an example. We describe how the ECM directs mammary gland formation and function, and discuss how a cell's inability to interpret these signals - whether as a result of genetic insults or physicochemical alterations in the ECM - disorganizes the gland and promotes malignancy. By restoring context and forcing cells to properly interpret these native signals, aberrant behavior can be quelled and organization re-established. Traditional imaging approaches have been a key complement to the standard biochemical, molecular, and cell biology approaches used in these studies. Utilizing imaging modalities with enhanced spatial resolution in live tissues may uncover additional means by which the ECM regulates tissue structure, on different length scales, through its pericellular organization (short-scale) and by biasing morphogenic and morphostatic gradients (long-scale).

Ghajar, Cyrus M; Bissell, Mina J

2008-10-23T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Neutron-induced Mammary Neoplasms in the Rat  

Science Journals Connector (OSTI)

...Fission neutrons were appropriately...y-rays or thermal neutrons (12). The neutron fission spectrum (mean energy, about 1...changing the neutron energy from the fission spectrum to 540 keV...the types of induced mammary neoplasms...

Howard H. Vogel, Jr. and Robert Zaldvar

1972-05-01T23:59:59.000Z

102

Identification of the Apoptosis Activation Cascade Induced in Mammary Carcinomas by Energy Restriction  

Science Journals Connector (OSTI)

...Induced in Mammary Carcinomas by Energy Restriction Henry J. Thompson...University, Fort Collins, Colorado Energy restriction (ER) inhibits...17: 3237-3245, 1998. 33 Green D. R. Apoptotic pathways...induced in mammary carcinomas by energy restriction. | Energy restriction...

Henry J. Thompson; Zongjian Zhu; and Weiqin Jiang

2004-02-15T23:59:59.000Z

103

No strings attached: new insights into epithelial morphogenesis  

Science Journals Connector (OSTI)

What regulates epithelial cell bending during early development? Lance Davidson comments on a paper from Zhirong Bao and colleagues where cell ingression in worm gastrulation is driven by dynamic cytoskeletal changes and cortical flow, challenging previous purse-string constriction models.

Lance A Davidson

2012-12-20T23:59:59.000Z

104

Densely Ionizing Radiation Acts via the Microenvironment to Promote Aggressive Trp53-Null Mammary Carcinomas  

Science Journals Connector (OSTI)

...ionizing radiation. The unique pattern of energy deposition incurred by particle traversal...epithelial cells were positive for K18 (green).Nuclei were counterstained with DAPI...positive either for K18 or K14 (n = 20, green; D), K14+18 type with K14 and K18...

Irineu Illa-Bochaca; Haoxu Ouyang; Jonathan Tang; Christopher Sebastiano; Jian-Hua Mao; Sylvain V. Costes; Sandra Demaria; and Mary Helen Barcellos-Hoff

2014-12-01T23:59:59.000Z

105

Gene expression analysis of human primary prostate epithelial and  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

expression analysis of human primary prostate epithelial and expression analysis of human primary prostate epithelial and fibroblast cell cultures to an acute dose of 10cGy J. Tyson McDonald Steward St. Elizabeth’s Medical Center Abstract Primary tissue represents a better model for studies than immortalized cell lines that are adapted to culture conditions and may no longer reflect a realistic biological state. In this study, normal tissues from clinically indicated robotic-assisted laparoscopic radical prostatectomy were grossly identified, sectioned into frozen or formalin fixed samples, and processed as primary cultures. Normal epithelial and fibroblast primary cell cultures were derived from regions of normal tissue, as confirmed by analysis on adjacent tissue by hematoxylin and eosin staining, were exposed to acute

106

GFP Expression in the Mammary Gland for Imaging of Mammary Tumor Cells in Transgenic Mice  

Science Journals Connector (OSTI)

...MMTV) to directly drive expression of GFP, we...was relatively weak and variable in frequency. By crossing MMTV-GFP...terminal repeat is used to drive selective expression...MMTV) to directly drive expression of GFP, we...

Fayyaz Ahmed; Jeffrey Wyckoff; Elaine Y. Lin; Weigang Wang; Yarong Wang; Lothar Hennighausen; Jun-ichi Miyazaki; Joan Jones; Jeffrey W. Pollard; John S. Condeelis; and Jeffrey E. Segall

2002-12-15T23:59:59.000Z

107

Spontaneous Metastasis from Primary C3H Mouse Mammary Tumors  

Science Journals Connector (OSTI)

...2 sublines of the C3H strain, the exogenous (milk transmit ted) mouse mammary tumor virus-infectedC3H/He and the aforemen...140-147, 1983. 20. Cam, Y., Caulet, T., Bellon, G., Poulin, G., Legros, M., and Pytlinska, M. Immunohistochemical...

Jan Vaage and James P. Harlos

1987-01-15T23:59:59.000Z

108

Cytochemical Studies on the Mouse Mammary Tumor Virus  

Science Journals Connector (OSTI)

...Elaboration in C3H Mouse Mammary Car cinoma as Observed by...and Bernhard, W. Water Soluble Embedding Media...Hydroxypropyl Methacrylate, a New Water-miscible Embedding Medium...Bernhard, W. The Use of Water-soluble Glycol Methacrylate...Smith FIG. 5. Low-power micrograph showing the...

Gilbert H. Smith

1967-11-01T23:59:59.000Z

109

Original article Sensitization of the bovine mammary gland to  

E-Print Network [OSTI]

(ReceivedI October I )96; accepted 4 February 1997) Summary ― The effect of repeated infusions intramammary infusions of E cnli endotoxin (33 pg) 24 h apart in the same mammary quarter. Along with the second infusion, the cows received one dose of endotoxin in the contralateral quarter. Milk was collected

Boyer, Edmond

110

REVIEW Open Access Epithelial to mesenchymal transition as a biomarker  

E-Print Network [OSTI]

subjected to injury may undergo similar transformations and thus provide new fibroblasts in the interstitium epithelial cells aberrantly expressing fibroblast-specific protein (FSP)1 in a model of mouse anti-tubular membrane disease [4]. This led Strutz et al. to hypothesize that some fibro- blasts might be derived from

Boyer, Edmond

111

Tumor Necrosis Factor-? Increases Reactive Oxygen Species by Inducing Spermine Oxidase in Human Lung Epithelial Cells: A Potential Mechanism for Inflammation-Induced Carcinogenesis  

Science Journals Connector (OSTI)

...increasing levels of positive cell cycle regulators, as well as components...exposure to TNF-alpha, a general mediator of inflammation...molecular mechanism by which general inflammation can contribute...intratracheal administration of diesel exhaust particles. Carcinogenesis...

Naveen Babbar and Robert A. Casero, Jr.

2006-12-01T23:59:59.000Z

112

A Novel Human Cytomegalovirus Glycoprotein, gpUS9, Which Promotes Cell-to-Cell Spread in Polarized Epithelial Cells, Colocalizes with the Cytoskeletal Proteins E-Cadherin and F-Actin  

Science Journals Connector (OSTI)

...solubility. Immunofluorescence laser scanning confocal microscopy...of human fibroblasts (HF) in culture (reviewed...ARPE-19) cells by fusion of the virion envelope...Immunofluorescence laser scanning confocal microscopy...cultures of nonpolarized HF (, , ). Since a substantial...

Ekaterina Maidji; Sharof Tugizov; Gerardo Abenes; Thomas Jones; Lenore Pereira

1998-07-01T23:59:59.000Z

113

Quantitation of the Rate of Spontaneous Generation and Carcinogen-induced Frequency of Anchorage-independent Variants of Rat Tracheal Epithelial Cells in Culture  

Science Journals Connector (OSTI)

...alepithelial; EGV, enhanced growth variant; CFE, colony-forming efficiency; MNNG, N-methyl-W...equation M No. of colonies observed in agarose CFE in agarose of cells from isolated colonies...optimize conditions to obtain the maximum CFE and colony size of anchorage-independent...

David G. Thomassen; Paul Nettesheim; Thomas E. Gray; J. Carl Barrett

1985-04-01T23:59:59.000Z

114

Enhanced Induction of the Anchorage-independent Phenotype in Initiated Rat Tracheal Epithelial Cell Cultures by the Tumor Promoter 12-O-Tetradecanoylphorbol-13-acetate  

Science Journals Connector (OSTI)

...carioylphorbol-13-acetate; FBS, fe tal bovine serum; CFE, colony-forming efficiency; DMSO, dimethyl...exposure media was 0.2%. Cell Number and CFE Measurement. At Day 40, all cultures were...anchorage- independent growth by measuring CFE in soft agarose by a modified MacPherson...

Vernon E. Steele; Diane K. Beeman; Paul Nettesheim

1984-11-01T23:59:59.000Z

115

Plastin3 Is a Novel Marker for Circulating Tumor Cells Undergoing the EpithelialMesenchymal Transition and Is Associated with Colorectal Cancer Prognosis  

Science Journals Connector (OSTI)

...under which the one-sided P value of 0.025 would have 80% power to detect a difference between the disease-free survival and...CellSearch system.Clin Cancer Res 2007;13:920-8. 7. Wind J , Tuynman JB, Tibbe AG, Swennenhuis JF, Richel DJ, van...

Takehiko Yokobori; Hisae Iinuma; Teppei Shimamura; Seiya Imoto; Keishi Sugimachi; Hideshi Ishii; Masaaki Iwatsuki; Daisuke Ota; Masahisa Ohkuma; Takeshi Iwaya; Naohiro Nishida; Ryunosuke Kogo; Tomoya Sudo; Fumiaki Tanaka; Kohei Shibata; Hiroyuki Toh; Tetsuya Sato; Graham F. Barnard; Takeo Fukagawa; Seiichiro Yamamoto; Hayao Nakanishi; Shin Sasaki; Satoru Miyano; Toshiaki Watanabe; Hiroyuki Kuwano; Koshi Mimori; Klaus Pantel; and Masaki Mori

2013-04-01T23:59:59.000Z

116

E-Print Network 3.0 - alveolar epithelial gp60 Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of the underlying mammary mesenchyme by the presumptive mammary epithelium to establish a bulb of ... Source: Scott, Matthew - Departments of Developmental Biology, Genetics, &...

117

Association of Rat p15INK4B/p16INK4 Deletions with Monosomy 5 in Kidney Epithelial Cell Lines but not Primary Renal Tumors  

Science Journals Connector (OSTI)

...Philadelphia, Pennsylvania 19107 [F. J. R.] wTI REPRESSIONOF bcl-2 AND c-myc A P2 start...8 0.6 0.4 0.2 WT1-KTS A 0 0 0 0 WTI+KTS 0 1 2 3 4 5 B Ratio of WT1 to bcl-2...WT1 "KThand WT1+KTS into HeLa cells. WTI "KTSand WT1+KTS both repressed CAT activity...

Dawn Fraser Knapek; Manuel Serrano; David Beach; David Trono; Cheryl L. Walker

1995-04-15T23:59:59.000Z

118

Effect of Intravenous Amino Acid Infusion on Leucine Oxidation Across the Mammary Gland  

E-Print Network [OSTI]

Effect of Intravenous Amino Acid Infusion on Leucine Oxidation Across the Mammary Gland) and the AA infusion periods. Although blood flow to the mammary gland and the arterial concen- tration of most AA other than leucine were increased by the AA infusion, milk and protein yields did not change

Bequette, Brian J.

119

S14 protein in breast cancer cells: Direct evidence of regulation by SREBP-1c, superinduction with progestin, and effects on cell growth  

SciTech Connect (OSTI)

Most breast cancers exhibit brisk lipogenesis, and require it for growth. S14 is a lipogenesis-related nuclear protein that is overexpressed in most breast cancers. Sterol response element-binding protein-1c (SREBP-1c) is required for induction of lipogenesis-related genes, including S14 and fatty acid synthase (FAS), in hepatocytes, and correlation of SREBP-1c and FAS expression suggested that SREBP-1c drives lipogenesis in tumors as well. We directly tested the hypothesis that SREBP-1c drives S14 expression and mediates lipogenic effects of progestin in T47D breast cancer cells. Dominant-negative SREBP-1c inhibited induction of S14 and FAS mRNAs by progestin, while active SREBP-1c induced without hormone and superinduced in its presence. Changes in S14 mRNA were reflected in protein levels. A lag time and lack of progestin response elements indicated that S14 and FAS gene activation by progestin is indirect. Knockdown of S14 reduced, whereas overexpression stimulated, T47D cell growth, while nonlipogenic MCF10a mammary epithelial cells were not growth-inhibited. These data directly demonstrate that SREBP-1c drives S14 gene expression in breast cancer cells, and progestin magnifies that effect via an indirect mechanism. This supports the prediction, based on S14 gene amplification and overexpression in breast tumors, that S14 augments breast cancer cell growth and survival.

Martel, Peter M. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Bingham, Chad M. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); McGraw, Charles J. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Baker, Christina L. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Morganelli, Peter M. [Department of Microbiology and Immunology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Meng, Marie Louise [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Armstrong, Jessica M. [Norris Cotton Cancer Center, Dartmouth Medical School (United States); Department of Physiology, Dartmouth Medical School (United States); Moncur, Joel T. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Kinlaw, William B. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States) and Norris Cotton Cancer Center, Dartmouth Medical School (United States)]. E-mail: william.kinlaw@hitchcock.org

2006-02-01T23:59:59.000Z

120

Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation  

Science Journals Connector (OSTI)

...oxygen and 37C in a 5% CO2 humidified atmosphere. Dishes were sealed with PetriSeal adhesive...homo-cis-interactions within its own cell plasma membrane. The combination of trans and...Epithelial cell-cell junctions and plasma membrane domains. Biochim. Biophys...

Lydia Aschauer; Leonhard N. Gruber; Walter Pfaller; Alice Limonciel; Toby J. Athersuch; Rachel Cavill; Abdulhameed Khan; Gerhard Gstraunthaler; Johannes Grillari; Regina Grillari; Philip Hewitt; Martin O. Leonard; Anja Wilmes; Paul Jennings

2013-04-22T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation  

Science Journals Connector (OSTI)

...homo-cis-interactions within its own cell plasma membrane. The combination of trans and...proximal-tubule phenotype. Published ahead of print 22 April 2013 ACKNOWLEDGMENTS: This work...Epithelial cell-cell junctions and plasma membrane domains. Biochim. Biophys...

Lydia Aschauer; Leonhard N. Gruber; Walter Pfaller; Alice Limonciel; Toby J. Athersuch; Rachel Cavill; Abdulhameed Khan; Gerhard Gstraunthaler; Johannes Grillari; Regina Grillari; Philip Hewitt; Martin O. Leonard; Anja Wilmes; Paul Jennings

2013-04-22T23:59:59.000Z

122

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary Genetic susceptibility to low-dose ionizing radiation in the mouse mammary gland as a means of understanding human risk for breast cancer Antoine M. Snijders Lawrence Berkeley National Laboratory Abstract Goal: Our goal is to develop an in vivo mechanistic model of genetic variation in the low-dose damage responses of mammary glands using inbred mice known to vary in their sensitivity to low-dose induced mammary gland cancer, and to develop molecular predictors for susceptibility or resistance to low-dose induced breast cancer. Background and Significance: It is increasingly believed that individuals differ in their genetic susceptibilities to environmental insults for diseases such as cancer. This concern is especially important for the large numbers of individuals receiving low-dose exposures in the nuclear energy

123

Effects of Physical Activity and Restricted Energy Intake on Chemically Induced Mammary Carcinogenesis  

Science Journals Connector (OSTI)

...Effects of Physical Activity and Restricted Energy Intake on Chemically Induced Mammary Carcinogenesis...little attention has been given to whether energy balance directed interventions designed to regulate body weight by increasing energy expenditure versus reducing energy intake...

Weiqin Jiang; Zongjian Zhu; Henry J. Thompson

2009-04-01T23:59:59.000Z

124

Tissue architecture: the ultimate regulator of breast epithelial function  

SciTech Connect (OSTI)

A problem in developmental biology that continues to take center stage is how higher organisms generate diverse tissues and organs given the same cellular genotype. In cell and tumor biology, the key question is not the production of form, but its preservation: how do tissues and organs maintain homeostasis, and how do cells within tissues lose or overcome these controls in cancer? Undoubtedly, mechanisms that maintain tissue specificity should share features with those employed to drive formation of the tissues. However, they are unlikely to be identical. At a simplistic level, developmental pathways may be thought of as a series of extremely rapid short-term events. Each new step depends on what came before, and the outcome is the organism itself at birth. All organs, with a few notable exceptions, such as the mammary gland and the brain, 'arrive' together and are complete when the organism is born. In mice and humans, these events occur in a mere 21 days and 9 months respectively. The stability of the differentiated state and the homeostasis of the organism, on the other hand, will last 40-110 times longer. How does the organism achieve this feat? How are tissues maintained? These questions also relate fundamentally to how tissues become malignant and, although not discussed here, to aging. While there is much literature on differentiation - loosely defined as the gain of a single or a series of functions - we know much less about the forces and the pathways that maintain organ morphology and function as a unit. This may be partly because it is difficult to study a tissue as a unit in vivo and there are few techniques that allow maintenance of organs in vitro long enough and in such a way as to make cell and molecular biology experiments possible. Techniques for culturing cells in three-dimensional gels (3D) as a surrogate for tissues, however, have been steadily improving and the method is now used by several laboratories. In this commentary we discuss the following: first, how our laboratory came to develop a model of the mammary gland acinus; second, what this model has told us about mechanisms that govern tissue specificity and malignancy; and third, possible directions for future studies. We summarize the evidence for the central role of ECM signaling in the maintenance of mammary function in culture and (more briefly) its role in tumorigenesis. This is followed by a discussion of the role that tissue architecture and tissue polarity (as opposed to cell polarity) may play in these processes. In an elegantly written and reasoned essay, Kirschner et al. coined the new science of developmental biology 'molecular vitalism'. They framed new concepts for self-organization as well as schemes for information flow in biological organization. Rao et al. reviewed and elaborated on differential-equation-based models of biochemical reaction networks and intracellular noise, with emphasis on bacteria and phage. Similarly, Hartwell et al. discussed the synergy between experiment and theory in elucidating 'modules' - collections of interacting molecules - and in unraveling how these modules collaborate to perform cellular functions such as signal transduction. We believe that many of these ideas will also be applicable to the maintenance of tissue specificity. As much as we agree with Kirschner et al. regarding the limitations of the machine analogy to biological systems, we conclude with thoughts on how we may proceed to model the complex tissue networks that govern breast tissue architecture. We suggest that our understanding of the structure and function of breast tissue would benefit from examining recent techniques for modeling large complex networks such as the World Wide Web and the Internet backbone among others.

Bissell, Mina J; Rizki, Aylin; Mian, Saira

2003-10-20T23:59:59.000Z

125

2-Deoxyglucose as an Energy Restriction Mimetic Agent: Effects on Mammary Carcinogenesis and on Mammary Tumor Cell Growth In vitro  

Science Journals Connector (OSTI)

...and Prevention 2-Deoxyglucose as an Energy Restriction Mimetic Agent: Effects on...University, Fort Collins, Colorado Dietary energy restriction (DER) is a potent inhibitor...Consequently, interest exists in identifying energy restriction mimetic agents (ERMAs...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; Henry J. Thompson

2005-08-01T23:59:59.000Z

126

Model-based Analysis of HER Activation in Cells Co-Expressing EGFR, HER2 and HER3.  

SciTech Connect (OSTI)

The HER/ErbB family of receptor tyrosine kinases drive critical responses in normal physiology and cancer, and the expression levels of the various HER receptors are critical determinants of clinical outcomes. HER activation is driven by the formation of various dimer complexes between members of this receptor family. The HER dimer types can have differential effects on downstream signaling and phenotypic outcomes. We constructed an integrated mathematical model of HER activation and trafficking to quantitatively link receptor expression levels to dimerization and activation. We parameterized the model with a comprehensive set of HER phosphorylation and abundance data collected in a panel of human mammary epithelial cells expressing varying levels of EGFR, HER2 and HER3. Although parameter estimation yielded multiple solutions, predictions for dimer phosphorylation were in agreement with each other. We validated the model using experiments where pertuzumab was used to block HER2 dimerization. We used the model to predict HER dimerization and activation patterns in a panel of epithelial cells lines with known HER expression levels. Simulations over the range of expression levels seen in various cell lines indicate that: i) EGFR phosphorylation is driven by HER1/1 and HER1/2 dimers, and not HER1/3 dimers, ii) HER1/2 and HER2/3 dimers both contribute significantly to HER2 activation with the EGFR expression level determining the relative importance of these species, and iii) the HER2/3 dimer is largely responsible for HER3 activation. The model can be used to predict phosphorylated dimer levels for any given HER expression profile. This information in turn can be used to quantify the potencies of the various HER dimers, and can potentially inform personalized therapeutic approaches.

Shankaran, Harish; Zhang, Yi; Tan, Yunbing; Resat, Haluk

2013-08-22T23:59:59.000Z

127

Effect of Dietary Palm Oils on Mammary Carcinogenesis in Female Rats Induced by 7,12-Dimethylbenz(a)anthracene  

Science Journals Connector (OSTI)

...Sciences Effect of Dietary Palm Oils on Mammary Carcinogenesis in...research grant from the Palm Oil Research Institute of Malaysia...from 200 to 230 Cat 2 C/min. Peak areas were quantified by an electronic...both contain 1447 DIETARY PALM OIL AND MAMMARY CANCER Table 1 Composition...

K. Sundram; H. T. Khor; Augustune S. H. Ong; and R. Pathmanathan

1989-03-15T23:59:59.000Z

128

Annexin A9 (ANXA9) biomarker and therapeutic target in epithelial cancer  

DOE Patents [OSTI]

Amplification of the ANXA9 gene in human chromosomal region 1q21 in epithelial cancers indicates a likelihood of both in vivo drug resistance and metastasis, and serves as a biomarker indicating these aspects of the disease. ANXA9 can also serve as a therapeutic target. Interfering RNAs (iRNAs) (such as siRNA and miRNA) and shRNA adapted to inhibit ANXA9 expression, when formulated in a therapeutic composition, and delivered to cells of the tumor, function to treat the epithelial cancer.

Hu, Zhi (El Cerrito, CA); Kuo, Wen-Lin (San Ramon, CA); Neve, Richard M. (San Mateo, CA); Gray, Joe W. (San Francisco, CA)

2012-06-12T23:59:59.000Z

129

E-Print Network 3.0 - alveolar type-ii cell Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

adhesion sites and collagen degradation peptides could induce alveolar type II... M CaCl2. Cell cultures Isolation and primary culture of alveolar epithelial cells Alveolar ......

130

Diagnostics of tumor cells by combination of Raman spectroscopy and microfluidics  

Science Journals Connector (OSTI)

Circulating epithelial tumor cells are of increasing importance for tumor diagnosis and therapy monitoring of cancer patients. The definite identification of the rare tumor cells...

Neugebauer, U; Dochow, S; Krafft, C; Bocklitz, T; Clement, J H; Popp, J

131

E-Print Network 3.0 - a549 cell cultures Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

PROLIFERATION AND PROTEIN EXPRESSION OF LUNG CANCER CELLS Summary: (NSCLC) cell lines. Methods: The epithelial lung carcinoma (A549 ) and a bronchioalveolar (NCI-H358......

132

Densely Ionizing Radiation Acts via the Microenvironment to Promote Aggressive Trp53-Null Mammary Carcinomas  

Science Journals Connector (OSTI)

...Radiation Acts via the Microenvironment to Promote Aggressive Trp53-Null Mammary...changes from radiation contribute strongly to carcinogenic potential. Densely ionizing...radiation chimera in which mice were exposed to densely ionizing 350 MeV/amu Si-particles...

Irineu Illa-Bochaca; Haoxu Ouyang; Jonathan Tang; Christopher Sebastiano; Jian-Hua Mao; Sylvain V. Costes; Sandra Demaria; Mary Helen Barcellos-Hoff

2014-12-01T23:59:59.000Z

133

Further Investigations on Induction of Mammary Cancer in Mice by Isografts of Hypophyseal Tissue  

Science Journals Connector (OSTI)

...in dependently to select the positive reactors "name ly: (a) The conversion of the...644586452472328Exp.QROUPVWX strates the reliability of the selection method employed. Mammary...described above are unsuitable for a complete analysis, since this investigation was primarily...

L. M. Boot; O. Mhlbock; G. Rpcke; and W. van Ebbenhorst Tengbergen

1962-07-01T23:59:59.000Z

134

Angiogenic Capacity of Preneoplastic Lesions of the Murine Mammary Gland as a Marker of Neoplastic Transformation  

Science Journals Connector (OSTI)

...many tissues studied. This process resulted in destruction of the...the important stages in this process remain undefined (16). In...currently thought to be a multistage process, in which the HAN nepre sents...of Mouse Mammary Tumor Strain MTG-B. Proc. Soc. Exptl. Biol...

Michael A. Gimbrone, Jr. and Pietro M. Gullino

1976-07-01T23:59:59.000Z

135

Expression of butyrophilin (Btn1a1) in lactating mammary gland is essential for the regulated  

E-Print Network [OSTI]

secretion of milk­lipid droplets Sherry L. Ogg*, Anne K. Weldon*, Lorraine Dobbie , Andrew J. H. Smith expressed in the lactating mammary gland and is secreted into milk in association with lipid droplets expression of Btn1a1 was either disrupted or eliminated, respectively. The regulated secretion of milk­lipid

Mather, Ian

136

Treatment of an Iatrogenic Left Internal Mammary Artery to Pulmonary Artery Fistula with a Bovine Pericardium Covered Stent  

SciTech Connect (OSTI)

We report a case with an acquired fistula between the left internal mammary artery and the pulmonary artery following coronary bypass surgery treated with a bovine pericardium covered stent. We also reviewed similar cases reported previously.

Heper, Gulumser [SSK Ihtisas Hospital, Department of Cardiology (Turkey)], E-mail: heperg@hotmail.com; Barcin, Cem; Iyisoy, Atila; Tore, Hasan F. [Gulhane Military Medical Academy, Department of Cardiology (Turkey)

2006-10-15T23:59:59.000Z

137

E-Print Network 3.0 - adult stromal cells Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the interactions between stromal and epithelial cells in tumorigenesis. Time-lapse fluorescent videos using mouse... interactions This session focused on reciprocal...

138

E-Print Network 3.0 - alveolar bone-derived cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Respiratory Physiology 2 Pulmonary Mechanics Summary: , 3rd Ed. Fig. 10.2 12;Surfactant Secretion by Type II Alveolar Epithelial Cells from multilamellar... basal laminabasement...

139

Comparative promoter activities of three endogenous copies of mouse mammary tumor virus  

E-Print Network [OSTI]

to 12 CC ~ o c 0 tu x MMTV M13 pLC1 ?(, a~ CAT pBR322 0/ I SV40 Figure 5. Diagram of pLC1. The plasmid pLC1 (Toohey et al. , 1986) contains an Intact LTR obtained from the C3H provirus as a Pst I restriction fragment. The plasmid also...COMPARATIVE PROMOTER ACTIVITIES OF THREE ENDOGENOUS COPIES OF MOUSE MAMMARY TUMOR VIRUS A Thesis by LAURA REID VILANDER Submitted to the Graduate College of Texas A&M University in partial fulfilment of the requirements for the degree...

Vilander, Laura Reid

1987-01-01T23:59:59.000Z

140

The effect of scaffold physical properties on endothelial cell function  

E-Print Network [OSTI]

Endothelial cells (EC) are ubiquitous - as vascular epithelial cells they line the inner surface of all vessels and are the contact surface with flowing blood. Macrovascular EC are the first line barrier between flowing ...

Murikipudi, Sylaja

2010-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Inhibitory effect of snake venom toxin from Vipera lebetina turanica on hormone-refractory human prostate cancer cell growth: induction of apoptosis through inactivation of nuclear factor ?B  

Science Journals Connector (OSTI)

...2):675-83] snake venom toxin|Vipera...implicated in tumor development and resistance to...cancer cell death. Snake venom toxins (SVT...in PBS) at room temperature for 2 h. The cells...during mammary gland development. Cell 2001;107...Chung KH, Kim DS. Snake venom disintegrin...

Dong Ju Son; Mi Hee Park; Sang Jin Chae; Soon Ok Moon; Jae Woong Lee; Ho Sueb Song; Dong Cheul Moon; Sang Sun Kang; Young Ee Kwon; and Jin Tae Hong

2007-02-01T23:59:59.000Z

142

Mechanisms of lead transport in two intestinal epithelial cell lines  

E-Print Network [OSTI]

recognized for as long as Pb has been used in society; however, Pb toxicity is still a problem today in humans and animals. Human exposure to Pb can occur by inhalation of Pb-contaminated dust, or more commonly, by ingesting Pb-contaminated water, food... to adults, children and young animals absorb greater amounts of a given dose of Pb; however, the reason for the increased absorption and the mechanisms by which Pb is absorbed in the immature gut are unclear. Using suckling and weanling rats, Henning...

Dekaney, Christopher Matthew

2012-06-07T23:59:59.000Z

143

Depleted uranium - induced malignant transformation in human lung epithelial cells.  

Science Journals Connector (OSTI)

...transmission of genetic damage by depleted uranium and tungsten alloy Alexandra Miller...The radioactive heavy metal, depleted uranium (DU), an alpha-particle emitter...fragments will affect the long-term health of offspring conceived by these...

Aldona A. Karaczyn; Hong Xie; and John P. Wise

2006-04-15T23:59:59.000Z

144

Depleted uranium - induced malignant transformation in human lung epithelial cells.  

Science Journals Connector (OSTI)

...Association for Cancer Research 15 April...Proc Amer Assoc Cancer Res, Volume 47, 2006 Depleted uranium - induced malignant...Proc Amer Assoc Cancer Res, Volume 47, 2006] 5215 Depleted uranium (DU) has been...

Aldona A. Karaczyn; Hong Xie; and John P. Wise

2006-04-15T23:59:59.000Z

145

Depleted uranium - induced malignant transformation in human lung epithelial cells.  

Science Journals Connector (OSTI)

...Washington, DC Abstract 3590: Depleted uranium-induced leukemia: Epigenetic...with leukemia development. Depleted uranium is used in military missions...Karvelisse Miller, Max Costa. Depleted uranium-induced leukemia: Epigenetic...

Aldona A. Karaczyn; Hong Xie; and John P. Wise

2006-04-15T23:59:59.000Z

146

Mammary Tumorigenesis in 7,12-Dimethybenzanthracene-treated C57BL DBA/2f F1 Mice  

Science Journals Connector (OSTI)

...analysis of M-406 mammary adenocarcinoma in F1 and F2 generations of a cross between resistant...characterize the tumor behavior in both F1 reciprocal hybrids [(CBi CBi-) and...M-406 grew exponentially in 45/48 F1 mice. Non statistical differences were...

Daniel Medina; Janet S. Butel; Susan H. Socher; Frances L. Miller

1980-02-01T23:59:59.000Z

147

A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland  

E-Print Network [OSTI]

% glutaraldehyde in 0.1 mol/l sodium cacodylate/HCl buffer of pH 7.27.4 at 4C for 48 h. After washing with distilled water (dH2O) for 20 min, the samples underwent secondary fixation in 1% osmium tetroxide in dH2O for 45 min at room temperature. Samples were...

Gordon, Katrina E; Binas, Bert; Chapman, Rachel S; Kurian, Kathreena M; Clarkson, Richard W E; Clark, A John; Birgitte Lane, E; Watson, Christine J

2000-03-07T23:59:59.000Z

148

Ultrasound Increases Nanoparticle Delivery by Reducing Intratumoral Pressure and Increasing Transport in Epithelial and EpithelialMesenchymal Transition Tumors  

Science Journals Connector (OSTI)

...Chemical Biology Ultrasound Increases Nanoparticle Delivery by Reducing Intratumoral Pressure...clinic can be used to enhance in vivo nanoparticle delivery to epithelial and EMT tumors...analysis of vascular permeability and nanoparticle accumulation in tumors. Acquisition...

Katherine D. Watson; Chun-Yen Lai; Shengping Qin; Dustin E. Kruse; Yueh-Chen Lin; Jai Woong Seo; Robert D. Cardiff; Lisa M. Mahakian; Julie Beegle; Elizabeth S. Ingham; Fitz-Roy Curry; Rolf K. Reed; Katherine W. Ferrara

2012-03-15T23:59:59.000Z

149

The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression  

E-Print Network [OSTI]

in breast cancer cell lines. Cancer Research 58, 1972-1977.human breast epithelial cell line isolated and propagated intumor and non-tumor cell lines established from patients

2008-01-01T23:59:59.000Z

150

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary glandas a means of understanding human risk for breast cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

susceptibility to low-dose ionizing radiation in the mouse mammary gland susceptibility to low-dose ionizing radiation in the mouse mammary gland as a means of understanding human risk for breast cancer Antoine M. Snijders 1 , Francesco Marchetti 1 , Ju Han 1 , Sandhya Bhatnagar 1 , Nadire Duru 1 , Zhi Hu 1 , Jian-Hua Mao 1 , Mina Bissell 1 , Joe Gray 1,2 , Gary H. Karpen 1 , Priscilla K. Cooper 1 and Andrew J. Wyrobek 1 1 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 2 Current affiliation: Biomedical Engineering, Oregon Health Science Univ, Portland, OR Goal: Our goal is to develop an in vivo mechanistic model of genetic variation in the low-dose damage responses of mammary glands using inbred mice known to vary in their sensitivity to low-dose induced mammary gland cancer, and to develop molecular predictors for susceptibility or resistance to low-dose induced breast cancer.

151

In Utero Exposure to Diethylstilbestrol (DES) or Bisphenol-A (BPA) Increases EZH2 Expression in the Mammary Gland: An Epigenetic Mechanism Linking Endocrine Disruptors to Breast Cancer  

Science Journals Connector (OSTI)

Diethylstilbestrol (DES) and bisphenol-A (BPA) are estrogen-like endocrine-disrupting chemicals that ... in adult women. Similarly, fetal exposure to BPA induces neoplastic changes in mammary tissue of ... of tum...

Leo F. Doherty; Jason G. Bromer; Yuping Zhou; Tamir S. Aldad

2010-06-01T23:59:59.000Z

152

Low Dose Radiation Research Program: Quantitative Analysis of Connexin  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Quantitative Analysis of Connexin Expression in Cultured Colonies Quantitative Analysis of Connexin Expression in Cultured Colonies Authors: B. Parvin, Q. Yang, R. L. Henshall-Powell and M.H. Barcellos Hoff We are studying the effects of ionizing radiation on the signaling between human mammary epithelial cells and the extracellular microenvironment. To do so we use an assay based on the ability of the cells to organize into three-dimensional acini when embedded into an extracellular matrix. Although tumorigenic and non-tumorigenic mammary epithelial cells are nearly indistinguishable when cultured as monolayers, their biological character readily diverge when tissue-specific morphogenesis is analyzed. Non-malignant human mammary epithelial cells (HMEC) cultured within a reconstituted basement membrane organize into acinar-like structures with

153

Pinkbar is an epithelial-specific BAR domain protein that generates planar membrane structures  

SciTech Connect (OSTI)

Bin/amphipysin/Rvs (BAR)-domain proteins sculpt cellular membranes and have key roles in processes such as endocytosis, cell motility and morphogenesis. BAR domains are divided into three subfamilies: BAR- and F-BAR-domain proteins generate positive membrane curvature and stabilize cellular invaginations, whereas I-BAR-domain proteins induce negative curvature and stabilize protrusions. We show that a previously uncharacterized member of the I-BAR subfamily, Pinkbar, is specifically expressed in intestinal epithelial cells, where it localizes to Rab13-positive vesicles and to the plasma membrane at intercellular junctions. Notably, the BAR domain of Pinkbar does not induce membrane tubulation but promotes the formation of planar membrane sheets. Structural and mutagenesis analyses reveal that the BAR domain of Pinkbar has a relatively flat lipid-binding interface and that it assembles into sheet-like oligomers in crystals and in solution, which may explain its unique membrane-deforming activity.

Pyklinen, Anette; Boczkowska, Malgorzata; Zhao, Hongxia; Saarikangas, Juha; Rebowski, Grzegorz; Jansen, Maurice; Hakanen, Janne; Koskela, Essi V.; Pernen, Johan; Vihinen, Helena; Jokitalo, Eija; Salminen, Marjo; Ikonen, Elina; Dominguez, Roberto; Lappalainen, Pekka (Helsinki); (Penn)

2013-05-29T23:59:59.000Z

154

Restoration of normal phenotype in cancer cells  

DOE Patents [OSTI]

A method for reversing expression of malignant phenotype in cancer cells is described. The method comprises applying {beta}{sub 1} integrin function-blocking antibody to the cells. The method can be used to assess the progress of cancer therapy. Human breast epithelial cells were shown to be particularly responsive. 14 figs.

Bissell, M.J.; Weaver, V.M.

1998-12-08T23:59:59.000Z

155

Restoration of normal phenotype in cancer cells  

DOE Patents [OSTI]

A method for reversing expression of malignant phenotype in cancer cells is described. The method comprises applying .beta..sub.1 integrin function-blocking antibody to the cells. The method can be used to assess the progress of cancer therapy. Human breast epithelial cells were shown to be particularly responsive.

Bissell, Mina J. (Berkeley, CA); Weaver, Valerie M. (Oakland, CA)

1998-01-01T23:59:59.000Z

156

Quantitative studies of EGFR autocrine induced cell signaling and migration  

E-Print Network [OSTI]

Epidermal growth factor (EGF) receptor autocrine and/or paracrine signaling plays an important role in normal epithelial cell proliferation, survival, adhesion and migration. Aberrant expression of the EGF receptor and its ...

Joslin, Elizabeth Jane

2007-01-01T23:59:59.000Z

157

Internal Mammary Lymph Node Irradiation Contributes to Heart Dose in Breast Cancer  

SciTech Connect (OSTI)

We assessed the impact of internal mammary chain radiotherapy (IMC RT) to the radiation dose received by the heart in terms of heart dose-volume histogram (DVH). Thirty-six consecutive breast cancer patients presenting with indications for IMC RT were enrolled in a prospective study. The IMC was treated by a standard conformal RT technique (50 Gy). For each patient, a cardiac DVH was generated by taking into account the sole contribution of IMC RT. Cardiac HDV were compared according to breast cancer laterality and the type of previous surgical procedure, simple mastectomy or breast conservative therapy (BCT). The contribution of IMC RT to the heart dose was significantly greater for patients with left-sided versus right-sided tumors (13.8% and 12.8% for left-sided tumors versus 3.9% and 4.2% for right-sided tumors in the BCT group and the mastectomy group, respectively; p < 0.0001). There was no statistically significant difference in IMC contribution depending on the initial surgical procedure. IMC RT contributes to cardiac dose for both left-sided and right-sided breast cancers, although the relative contribution is greater in patients with left-sided tumors.

Chargari, Cyrus [Department of Radiotherapy, Institut Gustave Roussy, Villejuif (France); Department of Radiotherapy and Medical Oncology, Hopital d'Instruction des Armees du Val-de-Grace, Paris (France); Castadot, Pierre [Department of Radio-Oncology, Institut Jules Bordet, Brussels (Belgium); MacDermed, Dhara [Department of Radiation and Cellular Oncology, University of Chicago, Chicago, IL (United States); Vandekerkhove, Christophe [Department of Medical Physics, Institut Jules Bordet, Brussels (Belgium); Bourgois, Nicolas; Van Houtte, Paul [Department of Radio-Oncology, Institut Jules Bordet, Brussels (Belgium); Magne, Nicolas, E-mail: nicolas.magne@igr.f [Department of Radiotherapy, Institut Gustave Roussy, Villejuif (France); Department of Radio-Oncology, Institut Jules Bordet, Brussels (Belgium)

2010-10-01T23:59:59.000Z

158

Cell-cell fusion induced by measles virus amplifies the type I interferon1 F. Herschke1  

E-Print Network [OSTI]

1 Cell-cell fusion induced by measles virus amplifies the type I interferon1 response2 3 F and $ These authors equally contributed to this work18 19 Running title: Amplified interferon by virus-induced fusion derived from human epithelial cells or mature conventional dendritic cells (mDC). Both33 fusion and IFN

Paris-Sud XI, Université de

159

Low Dose Radiation Research Program: Mary Helen Barcellos-Hoff - 2003  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

DOE Low Dose Radiation Program Workshop IV DOE Low Dose Radiation Program Workshop IV Abstract Title: TGF-β Protects Human Mammary Epithelial Cells from Radiation-Induced Centrosome Amplification Authors: Mary Helen Barcellos-Hoff, Bahram Parvin, Anna C. Erickson and Rishi Gupta Institutions: Department of Cell and Molecular Biology, Life Sciences Division, Ernest Orlando Lawrence, Berkeley National Laboratory, Berkeley, California In recent studies we have shown that ionizing radiation (IR), a known carcinogen of human and murine mammary gland, compromises human mammary epithelial cell (HMEC) polarity and multicellular organization in a manner characteristic of neoplastic progression through a heritable, non-mutational mechanism (1). Thus, when all cells are irradiated with a significant dose (2 Gy), the daughters of irradiated cells lose their

160

Human internal mammary artery contraction by isoprostaglandin F2? type-III (8-iso-prostaglandin F2?)  

Science Journals Connector (OSTI)

Isoprostaglandin F2? type-III (formerly known as 8-iso-prostaglandin F2?) is produced in large quantities in vivo in clinical situations associated with oxidant stress such as atherosclerosis, hypercholesterolemia, and myocardial reperfusion. Isoprostaglandin F2? type-III may alter smooth muscle and platelet functions. The aim of this study was to evaluate the effects of isoprostaglandin F2? type-III on isolated human internal mammary arteries, and to characterise the signalling underlying mechanisms. In organ baths, concentration-dependent contractions of human internal mammary arteries were obtained in response to isoprostaglandin F2? type-III stimulation. The responses to isoprostaglandin F2? type-III were inhibited in a concentration-dependent manner by the thromboxane A2 receptor antagonist, GR 32191 ([1R-[1 ?(Z), 2?,3?,5 ?(+)-7-[[1,1?-biphenyl)-4-yl]methoxy]-3-hydroxy-2-(1-piperidinyl) cyclo pentyl]-4-4heptanoic acid], hydrochloride), 310?9 to 310?7 M). However, this effect was associated with a decreased maximal contraction. AH 6809 (6-isopropoxy-9-oxoxanthene-2-carboxylic acid, 10?6 to 310?5 M), an EP1-DP receptor antagonist had no effect on isoprostaglandin F2? type-III-induced contractions. The maximal responses to isoprostaglandin F2? type-III were significantly reduced in the presence of the cyclooxygenase inhibitor indomethacin (10?5 M) (Emax: 14720% vs. 21319% in control group, PTP receptor but not EP1-DP-receptor activation, (3) thromboxane A2 but not cysteinyl leukotrienes production is involved in the vascular effects of isoprostaglandin F2? type-III. Isoprostaglandin F2? type-III, produced at sites of free radical generation, may play an important role in internal mammary artery spasm in situations of oxidant stress such as coronary bypass surgery.

Jean-Luc Cracowski; Franoise Stanke-Labesque; Philippe Devillier; Olivier Chavanon; Mark Hunt; Claude Souvignet; Germain Bessard

2000-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Regulation of epithelial-mesenchymal transition and DNA damage responses by singleminded-2s  

E-Print Network [OSTI]

repressor Singleminded-2, is lost or repressed in approximately 70% of human breast tumors and has a profound influence on normal mammary development. In order to gain a better understanding of the mechanisms by which SIM2s restricts malignant transformation...

Laffin, Brian Edward

2009-05-15T23:59:59.000Z

162

Method for restoration of normal phenotype in cancer cells  

DOE Patents [OSTI]

A method for reversing expression of malignant phenotype in cancer cells is described. The method comprises applying .beta..sub.1 integrin function-blocking antibody to the cells. The method can be used to assess the progress of cancer therapy. Human breast epithelial cells were shown to be particularly responsive.

Bissell, Mina J. (Berkeley, CA); Weaver, Valerie M. (Oakland, CA)

2000-01-01T23:59:59.000Z

163

(CANCER RESEARCH 48, 1390-1397, March 15, 1988] Epoxide-metabolizing Enzymes in Mammary Gland and Liver from BALB/c Mice  

E-Print Network [OSTI]

(CANCER RESEARCH 48, 1390-1397, March 15, 1988] Epoxide-metabolizing Enzymes in Mammary Gland and Liver from BALB/c Mice and Effects of Inducers on Enzyme Activity1 Marilyn H. Suva, Roger N. Wixtrom.3.23) (EH) are hydrolytic enzymes which may play an important role in the activation and detoxification

Hammock, Bruce D.

164

Prenatal and prepubertal exposures to bisphenol A (BPA) induce genomic alterations in the rat mammary gland during the window of high susceptibility to carcinogenesis.  

Science Journals Connector (OSTI)

...and prepubertal exposures to bisphenol A (BPA) induce genomic alterations in the rat...2008; San Diego, CA 3155 Bisphenol A (BPA), a monomer used to manufacture polycarbonate...effect of prenatal or prepubertal exposure to BPA on the genomic signature of rat mammary...

Julia Pereira; Mario Medvedovic; Raquel Moral; Irma Russo; Coral Lamartiniere; Jose Russo

2008-05-01T23:59:59.000Z

165

Genetic Bases of Estrogen-Induced Tumorigenesis in the Rat: Mapping of Loci Controlling Susceptibility to Mammary Cancer in a Brown Norway ACI Intercross  

Science Journals Connector (OSTI)

...E2-induced pyometritis in the Brown Norway rat, to RNO5. Mamm Genome...McComb RD, Shull JD. Dietary energy restriction inhibits estrogen-induced...to mammary cancer in a Brown Norway x ACI intercross. | Exposure...strain and the unrelated Brown Norway (BN) rat strain. Whereas...

Beverly S. Schaffer; Cynthia M. Lachel; Karen L. Pennington; Clare R. Murrin; Tracy E. Strecker; Martin Tochacek; Karen A. Gould; Jane L. Meza; Rodney D. McComb; and James D. Shull

2006-08-01T23:59:59.000Z

166

Abstract 476: Mammary tumors in lipocalin-2 deficient MMTV-PyVT mice grow faster but show decreased metastasis to lung  

Science Journals Connector (OSTI)

...Mammary tumors in lipocalin-2 deficient MMTV-PyVT mice grow faster but show decreased metastasis to lung Heiman Chow 1 Pengcheng Fan 1 Aimin Xu 1 Wen Laun Wendy Hsiao 2 Yu Wang 1 1Department of Pharmacology and Pharmacy, The University of Hong Kong...

Heiman Chow; Pengcheng Fan; Aimin Xu; Wen Laun Wendy Hsiao; Yu Wang

2011-01-10T23:59:59.000Z

167

Effects of Hypothyroidism on the Induction and Growth of Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in the Rat  

Science Journals Connector (OSTI)

...finding that hypothyroidism does not enhance DMBA-induced development...observed that hypothyroidism does not enhance the induction or...mammary carcinoma in the rat and does not alter its estrogen dependence...Kintz, B. L. Computational Handbook of Statistics, Ed. 2, pp...

A. David Goodman; Susan J. Hoekstra; and Patricia S. Marsh

1980-07-01T23:59:59.000Z

168

Effect of Irradiation on DNA Synthetic Period of the Mitotic Cycle in Cells  

Science Journals Connector (OSTI)

Kinetics of DNA synthesis in mitotic cycle of mouse corneal epithelial cells after single ?-irradiation (4Gy) at the end of S period was studied by the method of radioautography. Normally, S period of corneal...

I. P. Shabalkin; E. Yu. Grigoreva

2012-05-01T23:59:59.000Z

169

E-Print Network 3.0 - aldosterone modulates cell Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2002 Elsevier Science Inc. All rights reserved. Summary: . J. Cell. Biol. 83, 16- 32. Farman, N., Bonvalet, J.P., Secki, J.R., 1994. Aldosterone selectively... gland epithelial...

170

Recovery and evaluation of somatic cells from ovine and bovine semen for use in nuclear transfer  

E-Print Network [OSTI]

for chromosome analysis of cellsbefore using them for cloning experiments. In our attempts to clone animals, blastocyststage embryos were successfully produced using epithelial cells cultured from semen ofthree different bulls. However, no compact morulae...

Liu, Jie

2009-05-15T23:59:59.000Z

171

Cell Surface Markers on Epithelial-Burkitt Hybrid Cells Superinfected with Epstein-Barr Virus  

Science Journals Connector (OSTI)

...Foundation. Ronald Glaser 2 Gilbert Lenoir Soldano Ferrone...de-The Department of Microbiology and Specialized Cancer...Epithelium immunology microbiology Erythrocytes immunology...Virus1 Ronald Glaser,2 Gilbert Lenoir, Soldano Ferrane...de-Th Department of Microbiology and Specialized Cancer...

Ronald Glaser; Gilbert Lenoir; Soldano Ferrone; Michele A. Pellegrino; and Guy de-Th

1977-07-01T23:59:59.000Z

172

Interferon-? inhibits gastric carcinogenesis by inducing epithelial cell autophagy and T cell apoptosis  

E-Print Network [OSTI]

IFN-? mediates responses to bacterial infection and autoimmune disease, but it is also an important tumor suppressor. It is upregulated in the gastric mucosa by chronic Helicobacter infection; however, whether it plays a ...

Tu, Shui Ping

173

Estrogen-related Receptor ? and Estrogen-related Receptor ? Associate with Unfavorable and Favorable Biomarkers, Respectively, in Human Breast Cancer  

Science Journals Connector (OSTI)

...Fishers exact, P 0.006; Kruskal-Wallis ANOVA, P 0.021). ERRa levels...normal mammary epithelial cells (Kruskal-Wallis ANOVA, P 0.001). ERR overexpression...were tested by the nonparametric Kruskal-Wallis ANOVA (Figs. 1 2 3) . Associations...

Eric A. Ariazi; Gary M. Clark; Janet E. Mertz

2002-11-15T23:59:59.000Z

174

Changes in 2-Hydroxyestrone and 16?-Hydroxyestrone Metabolism with Flaxseed Consumption: Modification by COMT and CYP1B1 Genotype  

Science Journals Connector (OSTI)

...Fishers exact, P 0.006; Kruskal-Wallis ANOVA, P 0.021). ERRa levels...normal mammary epithelial cells (Kruskal-Wallis ANOVA, P 0.001). ERR overexpression...were tested by the nonparametric Kruskal-Wallis ANOVA (Figs. 1 2 3) . Associations...

Susan E. McCann; Jean Wactawski-Wende; Kari Kufel; James Olson; Bladimir Ovando; Susan Nowell Kadlubar; Warren Davis; Lisa Carter; Paola Muti; Peter G. Shields; Jo L. Freudenheim

2007-02-01T23:59:59.000Z

175

Effects of Phosphate Bond Energy on Nucleic Acid Stability in Tumor Homogenates  

Science Journals Connector (OSTI)

...A combination of detailed energy balance phenotyping and measures...AL+EX groups separately). Energy balance phenotyping Mice in...refs. 30, 31) using dual-energy X-ray absorptiometry (GE...mammary glands, including a mix of epithelial cells, adipocytes...

G. A. LePage

1949-05-01T23:59:59.000Z

176

Abstract 2872: Effects of ethanol and folate on peripubertal mouse mammary gland  

Science Journals Connector (OSTI)

...Long-term exposure of breast cell lines to ethanol affects the transcriptional signature...mammospheres. Here we aimed to determine whether ethanol, or its metabolite, acetaldehyde, stimulate...cell lines were incubated /- 25 mM ethanol and subjected to DNA microarray analysis...

Patricia A. Masso-Welch; Menachem E. Tobias; Shyam C. Vasantha Kumar; Judith Tamburlin; Stephen T. Koury

2011-01-10T23:59:59.000Z

177

Amplification and novel locations of endogenous mouse mammary tumor virus genomes in mouse T-cell lymphomas.  

Science Journals Connector (OSTI)

...except that transfers were hybridized to nick-translated PstI-B env probe. The position...MMTV-a genomes. One hypothesis that merits consideration is that MMTV-a sequences...acid to high specific activity in vitro by nick translation with DNA polymerase I. J...

J Dudley; R Risser

1984-01-01T23:59:59.000Z

178

Mislocalization of the Cell Polarity Protein Scribble Promotes Mammary Tumorigenesis and Is Associated with Basal Breast Cancer  

Science Journals Connector (OSTI)

...signaling and is required for normal zebrafish pronephros development.Proc Natl Acad Sci U S A 2009;106:8579-84. 7. Werme K , Wigerius M Johansson M.Tick-borne encephalitis virus NS5 associates with membrane protein Scribble and impairs interferon-stimulated...

Michael E. Feigin; S. Dipikaa Akshinthala; Kiyomi Araki; Avi Z. Rosenberg; Lakshmi B. Muthuswamy; Bernard Martin; Brian D. Lehmann; Hal K. Berman; Jennifer A. Pietenpol; Robert D. Cardiff; Senthil K. Muthuswamy

2014-06-01T23:59:59.000Z

179

Methyl p-Hydroxyphenyllactate and Nuclear Type II Binding Sites in Malignant Cells: Metabolic Fate and Mammary Tumor Growth  

Science Journals Connector (OSTI)

...Heterogeneity of estrogen-binding sites and the nuclear matrix. In: G. Maul (ed.). The Nuclear Matrix and the Nuclear Envelope, pp. 259-269. New York...inhibition on mitochondria! ATPase and energy-linked reactions in submitochondrial...

Barry M. Markaverich; Rebecca R. Gregory; MaryAnn Alejandro; Francis S. Kittrell; Daniel Medina; James H. Clark; Manju Varma; and Rajender S. Varma

1990-03-01T23:59:59.000Z

180

Effect of Energy Restriction on Cell Cycle Machinery in 1-Methyl-1-Nitrosourea-induced Mammary Carcinomas in Rats  

Science Journals Connector (OSTI)

...libitum fed (control); 40% energy ER continuously; and 40% ER...was annealed with GEAprimer Mix at 70C for 3 min and cooled...these effects are reversed by energy repletion. As an initial effort...abbreviations used are: ER, energy restriction; ER-REP, energy...

Weiqin Jiang; Zongjian Zhu; and Henry J. Thompson

2003-03-15T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Nucleic Acid Content and Nuclear Chromatin Structure of Human Bladder Cell Culture Lines as Studied by Flow Cytofluorometry  

Science Journals Connector (OSTI)

...characterizing tissue culture cell lines, as we have done home,and perhaps in distinguishing benign and malignant epithelial...Sharpless, T., and Darzynkiewicz, z. Urinary Cytology Automation. Preliminary Studies with Acridine Orange Stain and Flow-Through...

Myron R. Melamed; Zbigniew Darzynkiewicz; Frank Traganos; and Thomas K. Sharpless

1977-04-01T23:59:59.000Z

182

The role of ozone in tracheal cell transformation  

SciTech Connect (OSTI)

This project examined the potential role of ozone as a respiratory carcinogen by characterizing its ability to induce or modulate the preneoplastic transformation of rat tracheal epithelial cells. The chemical reactivity of ozone and the types of damage it can cause suggest that it may have a role in environmental carcinogenesis. Few other studies have examined the direct cytotoxic or transforming effects of ozone after in vivo or in vitro exposure of cells, and no studies have been reported on the comparative effects of ozone on respiratory cells exposed in vivo or in vitro. The induction of early preneoplastic changes in populations of rat tracheal epithelial cells by carcinogens can be detected and quantified in vitro after exposures in vivo or in vitro of tracheal epithelial cells. This cell culture and transformation system was used to characterize the transforming potency of ozone. Tracheal epithelial cells were isolated from Fischer-344/N rats that had been exposed for six hours per day, five days per week for one, two, or four weeks to 0, 0.12, 0.5, or 1.0 parts per million (ppm)* ozone (sea-level equivalents). Cell populations were examined in culture for increases in the frequency of preneoplastic variants. Rats exposed to ozone did not exhibit an increase in the frequency of preneoplastic tracheal cells, although exposed tracheas did exhibit dose-dependent morphological changes. Rat tracheal epithelial cells were given single, 40-minute in vitro exposures to concentrations of ozone that did not result in any detectable decrease in colony-forming efficiency.

Thomassen, D.G.; Harkema, J.R.; Sun, J.D.; Stephens, N.D.; Griffith, W.C. (Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States))

1992-04-01T23:59:59.000Z

183

Effects of Physical Activity and Restricted Energy Intake on Chemically Induced Mammary Carcinogenesis  

Science Journals Connector (OSTI)

...Thompson HJ. Effect of energy restriction on cell...biological research. New York: W.H. Freeman...comparison procedures. New York (NY): John Wiley...statistical methods. New York: McGraw-Hill Publishing...Mechanisms by which energy restriction inhibits...

Weiqin Jiang; Zongjian Zhu; and Henry J. Thompson

2009-04-01T23:59:59.000Z

184

Senescence-Associated Secretory Phenotypes Reveal Cell-Nonautonomous Functions of Oncogenic RAS and the p53 Tumor Suppressor  

SciTech Connect (OSTI)

Cellular senescence suppresses cancer by arresting cell proliferation, essentially permanently, in response to oncogenic stimuli, including genotoxic stress. We modified the use of antibody arrays to provide a quantitative assessment of factors secreted by senescent cells. We show that human cells induced to senesce by genotoxic stress secrete myriad factors associated with inflammation and malignancy. This senescence-associated secretory phenotype (SASP) developed slowly over several days and only after DNA damage of sufficient magnitude to induce senescence. Remarkably similar SASPs developed in normal fibroblasts, normal epithelial cells, and epithelial tumor cells after genotoxic stress in culture, and in epithelial tumor cells in vivo after treatment of prostate cancer patients with DNA-damaging chemotherapy. In cultured premalignant epithelial cells, SASPs induced an epithelial-mesenchyme transition and invasiveness, hallmarks of malignancy, by a paracrine mechanism that depended largely on the SASP factors interleukin (IL)-6 and IL-8. Strikingly, two manipulations markedly amplified, and accelerated development of, the SASPs: oncogenic RAS expression, which causes genotoxic stress and senescence in normal cells, and functional loss of the p53 tumor suppressor protein. Both loss of p53 and gain of oncogenic RAS also exacerbated the promalignant paracrine activities of the SASPs. Our findings define a central feature of genotoxic stress-induced senescence. Moreover, they suggest a cell-nonautonomous mechanism by which p53 can restrain, and oncogenic RAS can promote, the development of age-related cancer by altering the tissue microenvironment.

Copp, Jean-Philippe; Patil, Christopher; Rodier, Francis; Sun, Yu; Munoz, Denise; Goldstein, Joshua; Nelson, Peter; Desprez, Pierre-Yves; Campisi, Judith

2008-10-24T23:59:59.000Z

185

Measurements of scattering and absorption in mammalian cell suspensions  

SciTech Connect (OSTI)

During the past several years a range of spectroscopies, including fluorescence and elastic-scatter spectroscopy, have been investigated for optically based detection of cancer and other tissue pathologies. Both elastic-scatter and fluorescence signals depend, in part, on scattering and absorption properties of the cells in the tissue. Therefore an understanding of the scattering and absorption properties of cells is a necessary prerequisite for understanding and developing these techniques. Cell suspensions provide a simple model with which to begin studying the absorption and scattering properties of cells. In this study we have made preliminary measurements of the scattering and absorption properties of suspensions of mouse mammary carcinoma cells (EMT6) over a broad wavelength range (380 nm to 800 nm).

Mourant, J.R.; Johnson, T.M.; Freyer, J.P.

1996-03-01T23:59:59.000Z

186

Using ex vivo organ culture models as surrogates to investigate morphological and functional differences of mammary glands derived from mouse strains that differ in cancer susceptibility to understand the underlying mechanisms of radiation sensitivity or resistance  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ex vivo organ culture models as surrogates to investigate morphological and functional ex vivo organ culture models as surrogates to investigate morphological and functional differences of mammary glands derived from mouse strains that differ in cancer susceptibility to understand the underlying mechanisms of radiation sensitivity or resistance Alvin Lo, Joni D. Mott, Jian-Hua Mao, and Mina J. Bissell Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley CA 94720 Goal: Within the Lawrence Berkeley National Laboratory's Low Dose SFA, as part of Project 2, we are using a systems genetics approach to determine the contribution of non-targeted and targeted radiation effects for risk of mammary carcinogenesis. The goal of this work is to characterize the mammary gland of the parental mouse strains, and the F1 and F2 generations used in these studies with respect to tissue

187

Snail modulates cell metabolism in MDCK cells  

SciTech Connect (OSTI)

Highlights: ? MDCK/snail cells were more sensitive to glucose deprivation than MDCK/neo cells. ? MDCK/snail cells had decreased oxidative phosphorylation, O{sub 2} consumption and ATP content. ? TCA cycle enzyme activity, but not expression, was lower in MDCK/snail cells. ? MDCK/snail cells showed reduced PDH activity and increased PDK1 expression. ? MDCK/snail cells showed reduced expression of GLS2 and ACLY. -- Abstract: Snail, a repressor of E-cadherin gene transcription, induces epithelial-to-mesenchymal transition and is involved in tumor progression. Snail also mediates resistance to cell death induced by serum depletion. By contrast, we observed that snail-expressing MDCK (MDCK/snail) cells undergo cell death at a higher rate than control (MDCK/neo) cells in low-glucose medium. Therefore, we investigated whether snail expression influences cell metabolism in MDCK cells. Although gylcolysis was not affected in MDCK/snail cells, they did exhibit reduced pyruvate dehydrogenase (PDH) activity, which controls pyruvate entry into the tricarboxylic acid (TCA) cycle. Indeed, the activity of multiple enzymes involved in the TCA cycle was decreased in MDCK/snail cells, including that of mitochondrial NADP{sup +}-dependent isocitrate dehydrogenase (IDH2), succinate dehydrogenase (SDH), and electron transport Complex II and Complex IV. Consequently, lower ATP content, lower oxygen consumption and increased survival under hypoxic conditions was also observed in MDCK/snail cells compared to MDCK/neo cells. In addition, the expression and promoter activity of pyruvate dehydrogenase kinase 1 (PDK1), which phosphorylates and inhibits the activity of PDH, was increased in MDCK/snail cells, while expression levels of glutaminase 2 (GLS2) and ATP-citrate lyase (ACLY), which are involved in glutaminolysis and fatty acid synthesis, were decreased in MDCK/snail cells. These results suggest that snail modulates cell metabolism by altering the expression and activity of key enzymes. This results in enhanced glucose dependency and leads to cell death under low-glucose conditions. On the other hand, the reduced requirements for oxygen and nutrients from the surrounding environment, might confer the resistance to cell death induced by hypoxia and malnutrition.

Haraguchi, Misako, E-mail: haraguci@m3.kufm.kagoshima-u.ac.jp [Department of Biochemistry and Molecular Biology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Biochemistry and Molecular Biology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Indo, Hiroko P. [Department of Maxillofacial Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Maxillofacial Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Iwasaki, Yasumasa [Health Care Center, Kochi University, Kochi 780-8520 (Japan)] [Health Care Center, Kochi University, Kochi 780-8520 (Japan); Iwashita, Yoichiro [Department of Maxillofacial Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Maxillofacial Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Fukushige, Tomoko [Department of Dermatology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Dermatology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Majima, Hideyuki J. [Department of Maxillofacial Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Maxillofacial Radiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Izumo, Kimiko; Horiuchi, Masahisa [Department of Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Kanekura, Takuro [Department of Dermatology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Dermatology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Furukawa, Tatsuhiko [Department of Molecular Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Molecular Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan); Ozawa, Masayuki [Department of Biochemistry and Molecular Biology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)] [Department of Biochemistry and Molecular Biology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544 (Japan)

2013-03-22T23:59:59.000Z

188

Inhibition of 7,12-Dimethylbenz(a)anthracene-induced Tumors and DNA Adduct Formation in the Mammary Glands of Female Sprague-Dawley Rats by the Synthetic Organoselenium Compound, 1,4-Phenylenebis(methylene)selenocyanate  

Science Journals Connector (OSTI)

...terminated 16 wk later. The development of mammary tumors in...1 can inhibit tumor development induced by chemical...3.1.21.1), snake venom phosphodiesterase...in the diet at room temperature and at 4'C was confirmed...for 20 min at room temperature. The residual food...

Karam El-Bayoumy; Young-Heum Chae; Pramod Upadhyaya; Carol Meschter; Leonard A. Cohen; and Bandaru S. Reddy

1992-05-01T23:59:59.000Z

189

Plant cyclotides disrupt epithelial cells in the midgut of lepidopteran larvae  

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...DJ ( 2004 ) Biochemistry 43 : 5965 5975 . 5 Craik DJ ( 2006 ) Science 311 : 1563 1564 . 6 Gran L ( 1970 ) Meddelelser Norsk Farmaceutisk Selskap 32 : 173 180 . 7 Witherup KM ( 1994 ) J Nat Prod 57 : 1619 1625 . 8 Gustafson KR McKee TC Bokesch...

Barbara L. Barbeta; Alan T. Marshall; Amanda D. Gillon; David J. Craik; Marilyn A. Anderson

2008-01-01T23:59:59.000Z

190

Characterization of an Epithelial and a Tumor-associated Human Small Cell Lung Carcinoma Glycoprotein Antigen  

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...beled membrane proteins has led to the hope of many investi gators that their antibodies might be directed against such a protein...blood group determinant have been reported by other investi gators. Examples in other respiratory tract tumors include antibodies...

Robert Waibel; Carl J. O'Hara; Rolf A. Stahel

1987-07-15T23:59:59.000Z

191

E-Print Network 3.0 - amniotic epithelial cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Medicine ; Mathematics 18 Ultrastructure of the Reproductive System of the Black Swamp Snake (Seminatrix pygaea). VI. Anterior Summary: in all of the ducts at this time. The...

192

Tumor Angiogenesis and Its Possible Role in Intravasation of Colorectal Epithelial Cells  

Science Journals Connector (OSTI)

...in Fig. 1 . Vascular Counting. Slides were examined at low power magnification (40 and 100) to identify the areas of highest...Clin. Oncol., 12: 725-729, 1994. 19 Sales J. P., Wind P., Douard R., Cugnenc P. H., Loric S. Blood dissemination...

Yu-Wen Tien; King-Jen Chang; Yung-Ming Jeng; Po-Huang Lee; Ming-Shi Wu; Jaw-Town Lin; and Su-Ming Hsu

2001-06-01T23:59:59.000Z

193

Surface Membrane Nucleoside Triphosphatase Activity and Tumorigenicity of Cultured Liver Epithelial Cells  

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...centre Hospitalier Notre-Dame, Montr al, Canada H2L 4M1. Received May 4, 1976; accepted...DOpartementd'Anatomie,Universitdde Montr al,Canada (S. K.J, and Pasadena Foundation of...Biochemical and Histochemical Study of the Leed Method for Localization of Adenosine Triphosphate-Hydrolyzing...

Shuichi Karasaki and Tohru Okigaki

1976-12-01T23:59:59.000Z

194

Effects of Caloric Restriction and Dietary Fat on Epithelial Cell Proliferation in Rat Colon  

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...Canada. A semisynthetic high-corn oil diet enhanced the development...56.53.535.14.7High- corn oil diet1.019.92.83...a comparably equal source of energy as that of an equicaloric intake of corn oil, did not exert the latter's...

Gideon Steinbach; Siva P. Kumar; Bandaru S. Reddy; Martin Lipkin; and Peter R. Holt

1993-06-15T23:59:59.000Z

195

Polar delivery in plants; commonalities and differences to animal epithelial cells  

Science Journals Connector (OSTI)

April 2014 review-article Review 1001 33 58 2014 The Authors...in the acquisition of regulatory mechanisms that vary in...tight junctions. In this review, we summarize the similarities...shape their adult body plan already during embryogenesis...

2014-01-01T23:59:59.000Z

196

Characterizing ATP-dependent activation of inflammasome in gingival epithelial cells  

E-Print Network [OSTI]

26796. Yilmaz, O. , et al. , ATP-dependent activation of anYilmaz, O. , et al. , ATP scavenging by the intracellular1beta release by the ATP-gated P2X7 receptor. EMBO J. ,

Koo, Evonne

2010-01-01T23:59:59.000Z

197

E-Print Network 3.0 - apoptotic epithelial cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

in mechanosensory organ formation in the spider Cupiennius salei Summary: . The Kruskal-Wallis test was used to test for an overall effect of RNAi used on the number of...

198

Promiscuous mRNA splicing under the control of AIRE in medullary thymic epithelial cells  

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......This dataset contains RNA-Seq samples from brain, colon, heart, kidney, liver, lung, skeletal...and for all samples, except for one of the mouse brain samples (Mouse_B_Brain) the difference was highly statistically significant......

Peter Keane; Rhodri Ceredig; Cathal Seoighe

2014-12-01T23:59:59.000Z

199

Transformation of Epithelial Cells Stably Transfected with H2O2-generating Peroxisomal Urate Oxidase  

Science Journals Connector (OSTI)

...and Baudhuin, P. Microbodies (peroxisomes) and related particles. Physiol. Rev. 46: 323 "357,1966. 2. Tolbert, N...NY Acad. Sci., 386: 5 "12,1982. 6. Reddy, J. K., God, S. K., Nemali, M. R., Carrino, J. J., Laffler...

Ruiyin Chu; Yulian Lin; Kirthi C. Reddy; Jie Pan; M. Sambasiva Rao; Janardan K. Reddy; and Anjana V. Yeldandi

1996-11-01T23:59:59.000Z

200

Liver progenitor cells develop cholangiocyte-type epithelial polarity in three-dimensional culture.  

E-Print Network [OSTI]

143, 11011112. Strick-Marchand, H. , and Weiss, M. C. (et al. , 1998; Strick-Marchand and Weiss, 2002; Ader et

Tanimizu, Naoki; Miyajima, Atsushi; Mostov, Keith E

2007-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Abstract B41: Depleted uranium-induced oxidative stress in human bronchial epithelial cells  

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...Science of Cancer Health Disparities- Feb...AZ Abstract B41: Depleted uranium-induced oxidative...Science of Cancer Health Disparities- Feb...high deposits of uranium or tailings. There...occupational exposures to depleted uranium via military...

Monica Yellowhair; Leigh A. Henricksen; Aneesha Hossain; Kathleen Dixon; and R. Clark Lantz

2009-02-01T23:59:59.000Z

202

Abstract B41: Depleted uranium-induced oxidative stress in human bronchial epithelial cells  

Science Journals Connector (OSTI)

...Association for Cancer Research 1 February...The Science of Cancer Health Disparities...Abstract B41: Depleted uranium-induced oxidative...Carefree, AZ Cancer and mortality...deposits of uranium or tailings...exposures to depleted uranium via...

Monica Yellowhair; Leigh A. Henricksen; Aneesha Hossain; Kathleen Dixon; and R. Clark Lantz

2009-02-01T23:59:59.000Z

203

Abstract B41: Depleted uranium-induced oxidative stress in human bronchial epithelial cells  

Science Journals Connector (OSTI)

...Carefree, AZ Abstract B41: Depleted uranium-induced oxidative stress in...as occupational exposures to depleted uranium via military action. Cellular...to evaluate the toxicity of depleted uranium (DU) in its soluble and insoluble...

Monica Yellowhair; Leigh A. Henricksen; Aneesha Hossain; Kathleen Dixon; and R. Clark Lantz

2009-02-01T23:59:59.000Z

204

Vitamin D-Mediated Induction of Innate Immunity in Gingival Epithelial Cells  

Science Journals Connector (OSTI)

...Three-dimensional cultures of...indicate mean standard deviation...air-liquid interface (ALI) and...are means standard deviations...air-liquid interface culture system...cultures and measuring their viability...h in both standard monolayer and three-dimensional cultures was...

Laura McMahon; Kyell Schwartz; Ozlem Yilmaz; Eleith Brown; Lisa K. Ryan; Gill Diamond

2011-03-21T23:59:59.000Z

205

3D culture models of normal and malignant breast epithelial cells  

E-Print Network [OSTI]

3D culture models of normal and malignant breast epithelialcells; Lee et al. 3D culture models of normal and malignantFor correspondence: mjbissell@lbl.gov 3D culture models of

Lee, Genee Y.; Kenny, Paraic A.; Lee, Eva H.; Bissell, Mina J.

2006-01-01T23:59:59.000Z

206

Oncogenic manipulation and biologic selection for complete tumorigenic transformation of immortalized normal human bronchial epithelial cells  

Science Journals Connector (OSTI)

...manipulation soft agar colony forming efficiency (CFE) was 0.1%. With various oncogenic manipulations...higher KRASV12 and p53 knockdown increased CFE to 8%. None of these were tumorigenic...colonies were isolated and found to have CFE of 1015% with large colony numbers increased...

Mitsuo Sato; Woochang Lee; Luc Girard; Ruben D. Ramirez; David S. Shames; Adi F. Gazdar; Jerry W. Shay; John D. Minna

2006-04-15T23:59:59.000Z

207

Effect of Phorbol Esters on Clonal Cultures of Human, Hamster, and Rat Respiratory Epithelial Cells  

Science Journals Connector (OSTI)

...PDD, phorbol-12,13-didecanoate; CFE, colony-forming efficiency (the percent...scored under a dissecting micro scope and the CFE was calculated for each replicate. The...concentration of 30 nM or higher. The maximal CFE seen was 10-fold over control. CFE of...

Diane K. Beeman; Jill M. Siegfried; Marc J. Mass

1987-01-15T23:59:59.000Z

208

Low Dose Radiation Research Program: Multi-cellular Crosstalk in Radiation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

About this Project About this Project Multi-cellular Crosstalk in Radiation Damage Technical Abstracts 2006 Workshop: Low-LET Bystander Effects in Cells In Vitro Are Significantly Less Than Published For High-LET Radiation Blakely, E.A., Thompson, A.C., Chang, P., Schwarz, R.I., Bjornstad, K., Rosen, C., Wisnewski, C., and Mocherla, D. 2005 Workshop: X-ray Microbeam Bystander Studies with Human Mammary Epithelial Cells and Fibroblasts Blakely, E.A., Schwarz, R.I., Thompson, A.C., Bjornstad, K.A., Chang, P.Y., Rosen, C.J., Sudar, D., Romano, R., and Parvin, B. 2003 Workshop: 12.5 keV X-ray Microbeam Bystander Studies with Human Mammary Epithelial Cells and Fibroblasts Blakely, E.A., Schwarz, R.I., Thompson, A.C., Bjornstad, K.A., Chang, P.Y., Rosen, C.J., and Sudar, D. 2001 Workshop:

209

Continuum model of epithelial morphogenesis during Caenorhabditis elegans embryonic elongation  

Science Journals Connector (OSTI)

...defined using the fundamental assumption that...subjected to pulling forces and highly resilient...wormatlas.org/handbook/hypodermis...distribution of forces in the embryo...tensors express a fundamental strain measure...anisotropy is fundamental to elongate the...evenly distribute forces between cells...

2009-01-01T23:59:59.000Z

210

Epithelial-derived TGF-2 modulates basal and wound-healing subepithelial matrix homeostasis  

E-Print Network [OSTI]

conditions. We utilized an in vitro model of the epithelial-mesenchymal trophic unit in the human airways increases two- to threefold following scrape injury in a dose-depen- dent fashion and significantly enhances to epithe- lial injury. In the absence of the epithelium, exogenous active TGF- 2 (0­400 pg/ml) produces

George, Steven C.

211

The FASEB Journal Research Communication Calmodulin-dependent activation of the epithelial  

E-Print Network [OSTI]

calcium-dependent chloride channel TMEM16A Yuemin Tian,* Patthara Kongsuphol,* Martin Hug, Jiraporn-dependent activation of the epithelial calcium-dependent chloride channel TMEM16A. FASEB J. 25, 1058­1068 (2011). www 1­10). TMEM16A has 8 putative transmembrane do- mains (TMDs) and a p-loop between TMD 5 and TMD 6

Witzgall, Ralph - Naturwissenschaftliche Fakultät III

212

Organotypic Epithelial Raft Cultures as a Model for Evaluating Compounds against Alphaherpesviruses  

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...cultured in a three-dimensional organotypic raft...the air-liquid interface stratified and...the air-liquid interface. For VZV, the...accomplished by measuring viral titers by...for HSV and by measuring viral DNA load...demonstrated The three-dimensional epithelial raft...

Graciela Andrei; Joost van den Oord; Pierre Fiten; Ghislain Opdenakker; Chris De Wolf-Peeters; Erik De Clercq; Robert Snoeck

2005-11-01T23:59:59.000Z

213

Low Dose Radiation Research Program: Low Dose Response of Respiratory Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Response of Respiratory Cells in Intact Tissues and Reconstituted Response of Respiratory Cells in Intact Tissues and Reconstituted Tissue John Ford Department of Nuclear Engineering Texas A & M University Why this Project? Using the well-established rat trachea model to test the hypothesis that normal respiratory epithelial cells transmit signals to neighboring cells in response to very low dose radiation exposure. Project Goals By comparing the responses shown by cells in these normal rodent respiratory tissues to those seen for human respiratory epithelial cells in reconstituted tissue constructs, it will be possible to better understand the responds in human respiratory cells in vivo. These studies will characterize responses after exposure to a variety of radiation types and dose distributions. Experimental Approach

214

Genetic Profiling of Epithelial Cells Expressing E-Cadherin Repressors Reveals a Distinct Role for Snail, Slug, and E47 Factors in Epithelial-Mesenchymal Transition  

Science Journals Connector (OSTI)

...fact. We thank Amalia Montes, Vanesa Santos, and Raquel Marcos for their excellent...84-9. 11 Cano A, Perez-Moreno MA, Rodrigo I, et al. The transcription factor snail...16 Perez-Moreno MA, Locascio A, Rodrigo I, et al. A new role for E12/E47 in...

Gema Moreno-Bueno; Eva Cubillo; David Sarri; Hctor Peinado; Socorro Mara Rodrguez-Pinilla; Sonia Villa; Victoria Bols; Mireia Jord; Angels Fabra; Francisco Portillo; Jos Palacios; and Amparo Cano

2006-10-01T23:59:59.000Z

215

Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin  

Science Journals Connector (OSTI)

...author and source are credited. Hsp10 nuclear localization and changes in lung cells...isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation...epithelial cells|lung fibroblasts|nuclear localization| 2. Introduction The 10...

2014-01-01T23:59:59.000Z

216

ABSTRACT Title of Document: ROLE AND REGULATION OF AUTOPHAGY DURING DEVELOPMENTAL CELL DEATH IN DROSOPHILA MELANOGASTER  

E-Print Network [OSTI]

Two prominent morphological forms of programmed cell death occur during development, apoptosis and autophagic cell death. Improper regulation of cell death can lead to a variety of diseases, including cancer. Autophagy is required for survival in response to starvation, but has also been associated with cell death. It is unclear how autophagy is regulated under specific cell contexts in multi-cellular organisms, and what may distinguish autophagy function during cell survival versus cell death. Autophagic cell death is characterized by cells that die in synchrony, with autophagic vacuoles in the cytoplasm, and phagocytosis of the dying cells is not observed. However, little is known about this form of cell death. Autophagic cell death is observed during mammalian development, during regression of the corpus luteum and involution of the mammary and prostate glands. Autophagic cell death is also observed during development of the fruitfly Drosophila melanogaster, during larval salivary gland cell death. Drosophila is an excellent genetic model system to study developmental cell death in vivo. Cells use two main catabolic processes to degrade and recycle cellular

unknown authors

217

TSLP production by epithelial cells exposed to immunodeficiency virus triggers DC-mediated mucosal infection of CD4+ T cells  

Science Journals Connector (OSTI)

...short/0907347106 2 of 8 CCL17(pg/ml) 0 2000 4000 6000 8000 10000 M edia rT SLP rT SLP + -T SLP HIV +Sn HIV +Sn +-T SLP CCL22(pg/ml) 0 500 1000 1500 2000 2500 M edia rT SLP rT SLP + -T SLP HIV +Sn HIV + Sn +-T SLP a b Fig. S1. TSLP...

Danielle Fontenot; Hong He; Shino Hanabuchi; Pramod N. Nehete; Minying Zhang; Mikyoung Chang; Bharti Nehete; Yui-Hsi Wang; Yi-Hong Wang; Zhong-min Ma; Hai-Chon Lee; Steven F. Ziegler; Amy N. Courtney; Christopher J. Miller; Shao-Cong Sun; Yong-Jun Liu; K. Jagannadha Sastry

2009-01-01T23:59:59.000Z

218

Evaluation of Common Angling-Induced Sources of Epithelial Damage for Popular Freshwater Sport Fish using Fluorescein  

SciTech Connect (OSTI)

Angling is a popular recreational activity across the globe and a large proportion of fish captured by anglers are released due to voluntary or mandatory catch-and-release practices. The handling associated with hook removal and return of the fish to their environment can cause physical damage to the epidermal layer of the fish which may affect the condition and survival of released fish. This study investigated possible sources of epithelial damage associated with several different handling methods (i.e. landing net types, interactions with different boat floor surfaces, tournament procedures) commonly used in recreational angling for two popular freshwater sport fish species, largemouth bass (Micropterus salmoides) and northern pike (Esox lucius). Epithelial damage was examined using fluorescein, a non-toxic dye, which has been shown to detect latent epithelial damage. Northern pike exhibited extensive epithelial damage after exposure to several of the induced treatments (i.e., interaction with a carpeted surface, knotted nylon net, and line rolling) but relatively little epithelial damage when exposed to others (i.e., knotless rubber nets, smooth boat surfaces, or lip gripping devices). Largemouth bass did not show significant epithelial damage for any of the treatments, with the exception of fish caught in a semi-professional live release tournament. The detection of latent injuries using fluorescein can be an important management tool as it provides visual examples of potential damage that can be caused by different handling methods. Such visualizations can be used to encourage fish friendly angler behaviour and enhance the survival and welfare of released fish. It can also be used to test new products that are intended to or claim to reduce injury to fish that are to be released. Future research should evaluate the relationship between different levels of epithelial damage and mortality across a range of environmental conditions.

Colotelo, Alison HA; Cooke, Steven J.

2011-05-01T23:59:59.000Z

219

Lung Metastasis Fails in MMTV-PyMT Oncomice Lacking S100A4 Due to a T-Cell Deficiency in Primary Tumors  

Science Journals Connector (OSTI)

...behavior and promoting tumor invasion and metastasis. Contra wise, they can show pronounced antitumorigenic activity (2, 3...that promote metastasis. Cancer Cell 2008;13:23-35. 45 Alison MR , Lim S Houghton JM. Bone marrow-derived cells and epithelial...

Birgitte Grum-Schwensen; Jrg Klingelhfer; Mariam Grigorian; Kasper Almholt; Boye Schnack Nielsen; Eugene Lukanidin; and Noona Ambartsumian

2010-02-01T23:59:59.000Z

220

Murine Cell Lines Derived from Pten Null Prostate Cancer Show the Critical Role of PTEN in Hormone Refractory Prostate Cancer Development  

Science Journals Connector (OSTI)

...Pathobiology, and Genetics Murine Cell Lines Derived from Pten Null Prostate Cancer...receptor (AR)-positive prostate epithelial lines from intact conditional Pten knock-out...tumorigenesis is AR dependent. These cell lines will serve as useful tools for understanding...

Jing Jiao; Shunyou Wang; Rong Qiao; Igor Vivanco; Philip A. Watson; Charles L. Sawyers; Hong Wu

2007-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

A Congenic Line of the BALB/c Mouse Strain with the Endogenous Mouse Mammary Tumor Virus Proviral Gene Mtv-3: Tissue-specific Expression and Correlation with Resistance to Mouse Mammary Tumor Virus Infection and Tumorigenesis  

Science Journals Connector (OSTI)

...N-methyl-N-nitro-N-nitrosoguanidine by three exposure routes at different developmental...12-dimethylbenzanthracene by two exposure routes at different developmental...human rhabdomyosarcoma cell lines and tumour samples. J Pathol...pancreatic origin for the tumor. Transmission electron microscopy revealed...

Pierre Hainaut; Marc Castellazzi; Dominique Gonzales; Nathalie Clausse; Jo Hilgers; and Michel Crpin

1990-06-15T23:59:59.000Z

222

Comparative Evaluation of Four Presumptive Tests for Blood to Detect Epithelial Injury on Fish  

SciTech Connect (OSTI)

Current methods of fish epithelial injury detection are limited to gross macroscopic examination that has a subjective bias as well as an inability to reliably quantify the degree of injury. Fluorescein, a presumptive test for blood, has been shown to have the capability to detect and quantify fish epithelial injury. However, there are several other presumptive tests for blood (Bluestar*, phenolphthalein, and HemastixH) that may have benefits over the use of fluorescein, particularly for field research on wild fish. This study investigated the capabilities of these four tests to detect and quantify a variety of injuries commonly encountered by fish (abrasion, cuts, fin frays, and punctures) using the freshwater bluegill Lepomis macrochirus as a model. Fluorescein was consistently found to be the most reliable (i.e., detected the highest proportion of true positive results and rarely detected false positive reactions) of the four presumptive tests for blood compared. Further testing was conducted to examine the reliability of fluorescein. By 24 h after an injury was inflicted, the injury was no longer detectable by fluorescein, and when fluorescein was applied to an injured fish, the fluorescein was no longer detectable 3 h after application. In a comparison of two common anaesthetics used in fisheries research, there was no significant difference in the proportion of injury detected when 3- aminobenzoic acid ethyl ester methanesulfate (tricaine) was used compared with a clove oil and ethanol (1:9) solution. In summary, fluorescein was the most reliable presumptive test for blood examined in this study for the detection and quantification of recent (hours) fish epithelial injury.

Colotelo, Alison HA; Smokorowski, Karen; Haxton, Tim; Cooke, Steven J.

2014-06-01T23:59:59.000Z

223

Journal of Mammary Gland Biology and Neoplasia, Vol. 3, No. 3, 1998 Origin and Secretion of Milk Lipids  

E-Print Network [OSTI]

because the assembled lipid droplets are secreted from the cytoplasm enveloped by cellular membranes. In other cells, such as hepatocytes and enterocytes, lipid is secreted by exocytosis from membrane. Two possible mechanisms for lipid secretion have been proposed: an apical mechanism, in which lipid

Mather, Ian

224

Uptake and Clearance of 9,10-Dimethyl-1,2-benzanthracene-9-14C by Mammary Parenchymal Cells of the Rat  

Science Journals Connector (OSTI)

...4C in 1 ml sesame oil by gastric intubation...intracellular lipid attains a peak specific activity...4C in 1 ml sesame oil by gastric intubation...activity reaches a peak concentration of 48...4C in 1 ml sesame oil by gastric intubation...intra cellular lipid peaks and declines rapidly...

D. H. Janss and R. C. Moon

1970-02-01T23:59:59.000Z

225

Abstract 3691: Fish oil increases immune cell infiltration of tumors and reduces the incidence of mammary carcinogenesis in Her2neu mice.  

Science Journals Connector (OSTI)

...pattern on fatty acid composition of edible oil in Bangladesh: From preclinical studies...essential commodities, including edible oils, because of insufficient local production...million, and its improving economy. Edible oil is an energy-dense food nutrient providing...

William J. Turbitt; Shawntawnee D. Collins; Haifang Xu; Sharlene Washington; Cesar Aliaga; Karam El-Bayoumy; Andrea Manni; Connie J. Rogers

2013-04-15T23:59:59.000Z

226

Correlation of Retinoid Binding Affinity to Retinoic Acid Receptor ? with Retinoid Inhibition of Growth of Estrogen Receptor-positive MCF-7 Mammary Carcinoma Cells  

Science Journals Connector (OSTI)

...Bethesda, MD), M. I. Dawson (SRI International, CA), S. De Flora (University...Cancer, 75: 362-367. An international evaluation of the cancer preventive...Unit of Chemoprevention, International Agency for Research on Cancer...

Marcia I. Dawson; Wan-ru Chao; Polly Pine; Ling Jong; Peter D. Hobbs; Colette K. Rudd; Timothy C. Quick; Richard M. Niles; Xiao-kun Zhang; Angela Lombardo; Kathryn R. Ely; Braham Shroot; and Joseph A. Fontana

1995-10-01T23:59:59.000Z

227

Low Dose IR Creates an Oncogenic Microenvironment by Inducing Premature  

SciTech Connect (OSTI)

Introduction Much of the work addressing ionizing radiation-induced cellular response has been carried out mainly with the traditional cell culture technique involving only one cell type, how cellular response to IR is influenced by the tissue microenvironment remains elusive. By use of a three-dimensional (3D) co-culture system to model critical interactions of different cell types with their neighbors and with their environment, we recently showed that low-dose IR-induced extracellular signaling via the tissue environment affects profoundly cellular responses. This proposal aims at determining the response of mammary epithelial cells in a tissue-like setting.

Yuan, Zhi-Min [Harvard School of Public Health

2013-04-28T23:59:59.000Z

228

Abstract. Breast cancer is the most common cancer in women worldwide. Transformation of a normal cell to a malignant one  

E-Print Network [OSTI]

Abstract. Breast cancer is the most common cancer in women worldwide. Transformation of a normal regulators of growth. Biomarkers associated with cancer were examined in human breast epithelial cells transformed by high-LET radiation in the presence of 17?-estradiol. An established cancer model was used

229

SNAI1 Is Required for Tumor Growth and Lymph Node Metastasis of Human Breast Carcinoma MDA-MB-231 Cells  

Science Journals Connector (OSTI)

...indicate this fact. The authors thank Amalia Montes and Vanesa Santos for excellent technical assistance; and Drs. Virtanen and...Cell Biol 2000;2:84-9. 6 Cano A, Perez-Moreno MA, Rodrigo I, et al. The transcription factor snail controls epithelial-mesenchymal...

David Olmeda; Gema Moreno-Bueno; Juana M. Flores; Angels Fabra; Francisco Portillo; and Amparo Cano

2007-12-15T23:59:59.000Z

230

Inflammatory response of lung cells exposed to whole, filtered, and  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Inflammatory response of lung cells exposed to whole, filtered, and Inflammatory response of lung cells exposed to whole, filtered, and hydrocarbon denuded diesel exhaust Title Inflammatory response of lung cells exposed to whole, filtered, and hydrocarbon denuded diesel exhaust Publication Type Journal Article Year of Publication 2007 Authors Holder, Amara L., Donald Lucas, Regine Goth-Goldstein, and Catherine P. Koshland Journal Chemosphere Volume 70 Pagination 13-19 Date Published 09/2007 Keywords air-liquid interface, combustion particle, human bronchial epithelial cells, interleukin-8 Abstract In vitro studies with the organic extracts of diesel particles have suggested that hydrocarbons such as PAH may play a role in an inflammatory response, but these have been limited by the possible artifacts introduced in the particle collection and processing. In this study, we avoid these artifacts and use an activated carbon denuder to remove hydrocarbons from the exhaust stream to investigate their role in the inflammatory response. Human bronchial epithelial cells (16HBE140) were exposed at the air-cell interface to diluted and aged exhaust from a diesel generator operated at partial and no load conditions. When particles were removed with a filter before cell exposure, exhaust gases accounted for almost half of the response compared to the whole exhaust. Removal of gas phase and a portion of the particle phase hydrocarbons with the denuder decreased the interleukin-8 (IL-8) secretion to unexposed levels

231

Low Dose Radiation Research Program: Low Dose Response of Respiratory Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Low Dose Radiation Research Program: Low Dose Response of Respiratory Low Dose Radiation Research Program: Low Dose Response of Respiratory Cells in Intact Tissues and Reconstituted Tissue Constructs Authors: John Ford, Amy Maslowski, Alex Redd and Les Braby Institutions: Texas A&M University, College Station, TX We are developing a model of respiratory tissue using a perfusion culture system. We are using this system to quantify the effects of normal tissue architecture, and the interaction of epithelial cells with other cell types, on radiation-induced bystander effects. Tracheal tissue taken from young adult Fischer 344 rats is imbedded in a growth factor enriched agarose matrix. The chamber is designed to allow growth medium to periodically wash the epithelial surface of the tracheal lumen while maintaining the air-interface that is necessary for the normal

232

Force localization in contracting cell layers  

E-Print Network [OSTI]

Epithelial cell layers on soft elastic substrates or pillar arrays are commonly used as model systems for investigating the role of force in tissue growth, maintenance and repair. Here we show analytically that the experimentally observed localization of traction forces to the periphery of the cell layers does not necessarily imply increased local cell activity, but follows naturally from the elastic problem of a finite-sized contractile layer coupled to an elastic foundation. For homogeneous contractility, the force localization is determined by one dimensionless parameter interpolating between linear and exponential force profiles for the extreme cases of very soft and very stiff substrates, respectively. If contractility is sufficiently increased at the periphery, outward directed displacements can occur at intermediate positions, although the edge itself still retracts. We also show that anisotropic extracellular stiffness leads to force localization in the stiffer direction, as observed experimentally.

Carina M. Edwards; Ulrich S. Schwarz

2012-01-13T23:59:59.000Z

233

Small Intestinal Intraepithelial Lymphocytes Expressing CD8 and T Cell Receptor ?? Are Involved in Bacterial Clearance during Salmonella enterica Serovar Typhimurium Infection  

Science Journals Connector (OSTI)

...from purified iIELs or IECs using TRIzol (Invitrogen Life Technologies) reagent according to the protocol provided by the manufacturer...K Kawabata, JR McGhee, and H Kiyono. 1998. A mucosal intranet: intestinal epithelial cells down-regulate intraepithelial...

Zhiyuan Li; Cai Zhang; Zhixia Zhou; Jianhua Zhang; Jian Zhang; Zhigang Tian

2011-12-05T23:59:59.000Z

234

Purinergic receptors responsible for ATP-dependent ROS production and activation of inflammasomes in gingival epithelial cells  

E-Print Network [OSTI]

and G. Dahl, Probenecid, a gout remedy, inhibits pannexin-1Martinon F et al. (2006) Gout-associated uric acid crystalsincluding type I diabetes, gout, and many autoinflammatory

Ho, Marcus

2012-01-01T23:59:59.000Z

235

Purinergic receptors responsible for ATP-dependent ROS production and activation of inflammasomes in gingival epithelial cells  

E-Print Network [OSTI]

D. S. Kumararatne. 1997. ATP-induced killing of mycobacteria24. Cruz, C.M. , et al. , ATP activates a reactive oxygenYilmaz, O. , et al. , ATP scavenging by the intracellular

Ho, Marcus

2012-01-01T23:59:59.000Z

236

Abstract 276: Appalachian mountaintop mining particulate matter induces malignant transformation and tumorigenesis of human lung epithelial cells  

Science Journals Connector (OSTI)

...276: Appalachian mountaintop mining particulate matter induces malignant...Virginia (WV), the biggest coal mining state in Appalachia, ranks the third highest rate...that living near WV mountaintop coal mining (MTM) activities is a contributing...

Sudjit Luanpitpong; Juhua Luo; Travis Kneuckles; Michael Hendryx; and Yon Rojanasakul

2014-10-01T23:59:59.000Z

237

Neuroprotectin D1: A docosahexaenoic acid-derived docosatriene protects human retinal pigment epithelial cells from oxidative stress  

Science Journals Connector (OSTI)

...Jr. ( 2003 ) J. Neurochem. 87 , 297 -309. 14511107 8 Wheeler, T. G., Benolken, R. M. & Anderson, R. E...Leicester, U.K.) for the lentivirus construct, and to Katherine Gotlinger for expert technical assistance. This work was supported...

Pranab K. Mukherjee; Victor L. Marcheselli; Charles N. Serhan; Nicolas G. Bazan

2004-01-01T23:59:59.000Z

238

Roles of pilin and PilC in adhesion of Neisseria meningitidis to human epithelial and endothelial cells  

Science Journals Connector (OSTI)

Roles of pilin and PilC in adhesion of Neisseria meningitidis to...adhesiveness of strain 8013. Roles of pilin and PilC in adhesion of Neisseria meningitidis to...Bacterial Proteins 0 Oligonucleotide Probes 0 pilC protein, Neisseria gonorrhoeae 147680-16-8...

X Nassif; J L Beretti; J Lowy; P Stenberg; P O'Gaora; J Pfeifer; S Normark; M So

1994-01-01T23:59:59.000Z

239

Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence  

Science Journals Connector (OSTI)

...virtue of the associated adhesin protein PilC. To examine the structural and functional...PilE and copurifies with Tfp fibers in a PilC-dependent fashion. Purified Tfp from pilV mutants contain PilC adhesin at reduced levels. Taken together...

Hanne C. Winther-Larsen; Finn Terje Hegge; Matthew Wolfgang; Stanley F. Hayes; Jos P. M. van Putten; Michael Koomey

2001-01-01T23:59:59.000Z

240

The Effect of Croton Oil and Carcinogens on Mitosis and the Deoxyribonucleic Acid Content of Cervical Epithelial Cells  

Science Journals Connector (OSTI)

...an average of fifteen oil- immersion fields. In...the maximum absorption peak of the reddish purple...smears were mounted in oil with a refractive index...Chart 1). During the peak of mitotic fre quency...inflammatory response. Croton oil thread. "Peaksof mitotic...

D. G. Scarpelli and Emmerich von Haam

1958-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

A Role for Fibroblasts in Mediating the Effects of Tobacco-Induced Epithelial Cell Growth and Invasion  

Science Journals Connector (OSTI)

...J-P. Coppe), Department of Energy (DE-AC0376SF00098, J...K22 DE15316, M. Kang) and resources from NIH Biomedical Technology Resource Center at University of California...oxygen species mediate the solar ultraviolet radiation-dependent...

Jean-Philippe Coppe; Megan Boysen; Chung Ho Sun; Brian J.F. Wong; Mo K. Kang; No-Hee Park; Pierre-Yves Desprez; Judith Campisi; and Ana Krtolica

2008-07-01T23:59:59.000Z

242

Abstract 3464: Epigenetic mechanism is involved in depleted uranium-induced transformation in human lung epithelial cells  

Science Journals Connector (OSTI)

...Epigenetic mechanism is involved in depleted uranium-induced transformation in human...Southern Maine, Portland, ME. Depleted uranium (DU) is commonly used in military...research information on the potential health hazards of DU exposure. In our...

Hong Xie; Carolyne LaCerte; and John P. Wise

2010-04-15T23:59:59.000Z

243

Abstract 3464: Epigenetic mechanism is involved in depleted uranium-induced transformation in human lung epithelial cells  

Science Journals Connector (OSTI)

...Association for Cancer Research 15 April...Abstract 3590: Depleted uranium-induced leukemia...development. Depleted uranium is used in military...Max Costa. Depleted uranium-induced leukemia...Association for Cancer Research; 2013...

Hong Xie; Carolyne LaCerte; and John P. Wise

2010-04-15T23:59:59.000Z

244

Arp2/3 Inhibition Induces Amoeboid-Like Protrusions in MCF10A Epithelial Cells by Reduced Cytoskeletal-  

E-Print Network [OSTI]

Stricker3,4 , Kareem Sayegh3,4 , Clement Campillo5,6 , Margaret L. Gardel1,3,4 * 1 Institute required for its stabilization. Citation: Beckham Y, Vasquez RJ, Stricker J, Sayegh K, Campillo C, et al

Gardel, Margaret

245

Abstract A126: Risk factors for epithelial ovarian cancer by tumor dominance, a surrogate for cell of origin  

Science Journals Connector (OSTI)

...subgroup of patients with low risk and favorable outcome. Methods...hazard ratios (HR) for genomic risk were 4.9 (95%CI 1.2-20...prospectively evaluate if it is acceptable to withhold chemotherapy and...Her2-positive, genomic low risk patients. Citation Information...

Joanne Kotsopoulos; Margaret A. Gates; Jonathan L. Hecht; Bernard A. Rosner; Christopher C. Crum; and Shelley S. Tworoger

2010-01-01T23:59:59.000Z

246

miR149 Functions as a Tumor Suppressor by Controlling Breast Epithelial Cell Migration and Invasion  

Science Journals Connector (OSTI)

...inhibit some E2-activated genes. B, pie graph shows that among all E2-upregulated...belong to each represented category. C, pie graph shows that among all E2-downregulated...CARM1) were also activated by E2. B, pie graph shows that approximately 65% of CARM1...

Annabell Bischoff; Bettina Huck; Bettina Keller; Michaela Strotbek; Simone Schmid; Melanie Boerries; Hauke Busch; Dafne Mller; Monilola A. Olayioye

2014-09-15T23:59:59.000Z

247

Low Dose Radiation Research Program: 12.5 keV Xray Microbeam Bystander  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

12.5 keV Xray Microbeam Bystander Studies With Human Mammary 12.5 keV Xray Microbeam Bystander Studies With Human Mammary Epithelial Cells and Fibroblasts Authors: E. A. Blakely1, R. I. Schwarz1, A. C. Thompson2, K. A. Bjornstad1, P. Y. Chang1,3 C.J. Rosen1, and D. Sudar1 Institutions: Divisions of 1Life Sciences and 2Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA. and 3SRI International, Menlo Park, CA. We are using a novel x-ray Microprobe Beamline at the Advanced Light Source (ALS) at LBNL to investigate bystander effects of low doses in well characterized human mammary epithelial cells (HMEC) and human skin fibroblasts (HSF). The ALS facility is capable of producing a beam of 12.5 keV x-rays with a focussed spot size of __m_ and a wide range of doses and dose-rates. Unlike normal x-ray sources, this beam has a very small background of either low-

248

A Critical Examination of the Adaptive Response for Cytogenetic Damagein Human Cells, and Insights into the Adaptive Response Mechanism  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Critical Examination of the Adaptive Response for Cytogenetic Damage Critical Examination of the Adaptive Response for Cytogenetic Damage in Human Cells, and Insights into the Adaptive Response Mechanism Björn E. Rydberg, Torsten Groesser, Antoine Snijders, Kelly Trego, Ju Han, Do Yup Lee, Bahram Parvin, Trent Northen, Andrew J. Wyrobek, and Priscilla K. Cooper Berkeley Lab SFA P.I.: Gary Karpen Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 Goal: Task 1 of the Berkeley Lab SFA is designed to identify adaptive response (AR) mechanisms that may affect risk of developing radiation-induced cancer and to assess the linearity with dose of processes that influence mammary gland carcinogenesis. We use both in vitro and in vivo experimental systems in a parallelogram strategy. Our human cell culture

249

Evaluation of Yeast Cell Wall on Early Production Laying Hen Performance  

E-Print Network [OSTI]

from non-hydrolyzable oligo and polysaccharides increases the proliferation of the gut epithelial cells, thus increasing intestinal tissue weight, with changes in the overall morphology of intestinal mucosa (Niba et al., 2009; Bonos et al., 2011... and multiply within the digestive tract mucosa (Baurhoo et al., 2007b; Bonos et al., 2011; Jacobs, 2011). Types of prebiotics Most identified prebiotics are classified as carbohydrate oligosaccharides with differing molecular structure that are normally...

Hashim, Mohammed Malik Hashim 1981-

2012-11-08T23:59:59.000Z

250

4 T-cell responses and cellular immunity in coeliac disease  

Science Journals Connector (OSTI)

Increasing evidence points to a direct role for T cells in the mediation of the coeliac intestinal lesion. There is good evidence for increased local T-cell reactivity, manifest as increased in T-cell activation in the lamina propria and T-cell proliferation in the epithelial compartment. A likely scenario is that gluten elicits antigen-specific responses by lamina propria T helper cells, probably of the Th1 (inflammatory-mediator) subtype, leading to secretion of pro-inflammatory cytokines. Such cytokines may have direct effects on intestinal enterocytes, as well as mediating indirect effects by upregulation of MHC antigens and by enhancing the activity of cytolytic T cells. Although gluten-specific IEL responses have not been demonstrated by intraepithelial T lymphocytes (IELs), increasing evidence suggests that \\{IELs\\} can act as cytolytic effector cells and hence are likely to exert enteropathic effects under the influence of pro-inflammatory cytokines.

Ludwik K. Trejdosiewicz; Peter D. Howdle

1995-01-01T23:59:59.000Z

251

Breaking the epithelial polarity barrier in cancer: the strange case of LKB1/PAR-4  

Science Journals Connector (OSTI)

...link between cell polarity and energy metabolism. Physiol. Rev...Esteller, M , Avizienyte, E, Corn, PG, Lothe, RA, Baylin...regulates apoptosis in response to energy stress. Proc. Natl Acad...200702053 ) 80 Lee, JH , 2007 Energy-dependent regulation of cell...

2013-01-01T23:59:59.000Z

252

Berkeley Lab A to Z Index: M  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Macintosh Computer Backups Macintosh Computer Backups Macintosh User Group (LBNL-MUG) Mac support/MPSG (formerly known as the workstation group) Macromolecular Crystallography Facility (MCF) Mailing Addresses for Lab Mail (electronic); Email Support, Documentation, etc. Mail Services (Facilities Dep't.) malware (computer virus) protection and "How to Handle Suspected Malware" Mammary: Human Mammary Epithelial Cell (HMEC) Map: Berkeley Lab Global Talent Map Maps: Directions and Maps on How to Get to the Lab Maps: Berkeley Lab Interactive Site Map Maps: Berkeley Lab Printable Site Map Maps: Offsite Lab Shuttle Bus Map Maps: Onsite Lab Shuttle Bus Map Massage, Onsite Chair Mass Storage System (MSS) Material Safety Data Sheets: MSDS Materials Sciences Division (MSD) Materials Science: Technology Transfer

253

High LET radiatSpace Radiation Can Enhance the TGFβ InducedEpithelial-Mesenchymal Transitionion can enhance TGFβ induced EMT and cross-talk with ATM pathways  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Radiation Can Enhance the TGFβ Induced Radiation Can Enhance the TGFβ Induced Epithelial-Mesenchymal Transition Minli Wang 1 , Megumi Hada 1 , Janice Huff 1 , Janice M. Pluth 2 , Jennifer Anderson 3 , Peter O'Neill 3 and Francis A. Cucinotta 4 1 USRA Division of Life Sciences, Houston TX USA; 2 Lawrence Berkeley National Laboratory, Berkeley CA, USA, 3 Gray Institute for Radiation Oncology & Biology, University of Oxford, UK, 4 NASA, Lyndon B. Johnson Space Center, Houston TX, USA TGFβ is a key modulator of the Epithelial-Mesenchymal Transition (EMT), important in cancer progression and metastasis, which involve classic Smad or non-Smad signaling pathways, leading to the suppression of epithelial genes and promoted expression of mesenchymal proteins. Ionizing radiation was found to specifically induce expression of

254

Effect of Tight Junctional Resistance on Penetration of LLC-PK1 Epithelial Cell Layers by Metastatic B16-F10 Melanoma Cells  

Science Journals Connector (OSTI)

...were used to define the rate and degree of penetration of the various Ig forms...were used to define the rate and degree of penetration of the various Ig forms...were used to define the rate and degree of penetration of the various Ig forms...

Kathleen G. Conn and Karen A. Knudsen

1989-12-15T23:59:59.000Z

255

Coexpression of Helicobacter Pyloris Proteins CagA and HspB Induces Cell Proliferation in AGS Gastric Epithelial Cells, Independently from the Bacterial Infection  

Science Journals Connector (OSTI)

...Department of Medicine and Public Health, Section...Using a genomic library of H. pylori, we...establishes exact space-time repetition...of gastritis to a public health problem...Using a genomic library of H. pylori, we...Department of Medicine and Public Health, Section...

Antonio De Luca; Alfonso Baldi; Patrizia Russo; Anna Todisco; Lucia Altucci; Nicola Giardullo; Luigi Pasquale; Salvatore Iaquinto; Vittorio DOnofrio; Maria Caterina Parodi; Marco G. Paggi; and Gaetano Iaquinto

2003-10-01T23:59:59.000Z

256

Id-1 and Id-2 genes and products as markers of epithelial cancer  

DOE Patents [OSTI]

A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

Desprez, Pierre-Yves (El Cerrito, CA); Campisi, Judith (Berkeley, CA)

2011-10-04T23:59:59.000Z

257

Id-1 and Id-2 genes and products as markers of epithelial cancer  

DOE Patents [OSTI]

A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

Desprez, Pierre-Yves (El Cerrito, CA); Campisi, Judith (Berkeley, CA)

2008-09-30T23:59:59.000Z

258

Diacylglycerol kinase regulation of protein kinase D during oxidative stress-induced intestinal cell injury  

SciTech Connect (OSTI)

We recently demonstrated that protein kinase D (PKD) exerts a protective function during oxidative stress-induced intestinal epithelial cell injury; however, the exact role of DAG kinase (DGK){zeta}, an isoform expressed in intestine, during this process is unknown. We sought to determine the role of DGK during oxidative stress-induced intestinal cell injury and whether DGK acts as an upstream regulator of PKD. Inhibition of DGK with R59022 compound or DGK{zeta} siRNA transfection decreased H{sub 2}O{sub 2}-induced RIE-1 cell apoptosis as measured by DNA fragmentation and increased PKD phosphorylation. Overexpression of kinase-dead DGK{zeta} also significantly increased PKD phosphorylation. Additionally, endogenous nuclear DGK{zeta} rapidly translocated to the cytoplasm following H{sub 2}O{sub 2} treatment. Our findings demonstrate that DGK is involved in the regulation of oxidative stress-induced intestinal cell injury. PKD activation is induced by DGK{zeta}, suggesting DGK is an upstream regulator of oxidative stress-induced activation of the PKD signaling pathway in intestinal epithelial cells.

Song Jun [Department of Surgery, University of Texas Medical Branch, 301 University Blvd., Children's Hospital Room 3.200-E, Galveston, TX 77555-0353 (United States); Li Jing [Department of Surgery, University of Texas Medical Branch, 301 University Blvd., Children's Hospital Room 3.200-E, Galveston, TX 77555-0353 (United States); Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1048 (United States); Mourot, Joshua M. [Department of Surgery, University of Texas Medical Branch, 301 University Blvd., Children's Hospital Room 3.200-E, Galveston, TX 77555-0353 (United States); Mark Evers, B. [Department of Surgery, University of Texas Medical Branch, 301 University Blvd., Children's Hospital Room 3.200-E, Galveston, TX 77555-0353 (United States); Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1048 (United States); Chung, Dai H. [Department of Surgery, University of Texas Medical Branch, 301 University Blvd., Children's Hospital Room 3.200-E, Galveston, TX 77555-0353 (United States); Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1048 (United States)], E-mail: dhchung@utmb.edu

2008-10-17T23:59:59.000Z

259

Morphology, Natural History, and Enzyme Patterns in Mammary Tumors of the Rat Induced by 7,12-Dimethylbenz(a)anthracene  

Science Journals Connector (OSTI)

...population with regard to its natural history, the data in this study support the...cell pattern in predicting the natural history of individual tumors. Previous investigators...Theory and Methodology in Sci- ence and Engineering. Ch. 10, pp. 252-254. New York...

Francis L. Archer and Robert A. Orlando

1968-02-01T23:59:59.000Z

260

Context dependent reversion of tumor phenotype by connexin-43 expression in MDA-MB231 cells and MCF-7 cells: Role of ?-catenin/connexin43 association  

SciTech Connect (OSTI)

Connexins (Cx), gap junction (GJ) proteins, are regarded as tumor suppressors, and Cx43 expression is often down regulated in breast tumors. We assessed the effect of Cx43 over-expression in 2D and 3D cultures of two breast adenocarcinoma cell lines: MCF-7 and MDA-MB-231. While Cx43 over-expression decreased proliferation of 2D and 3D cultures of MCF-7 by 56% and 80% respectively, MDA-MB-231 growth was not altered in 2D cultures, but exhibited 35% reduction in 3D cultures. C-terminus truncated Cx43 did not alter proliferation. Untransfected MCF-7 cells formed spherical aggregates in 3D cultures, and MDA-MB-231 cells formed stellar aggregates. However, MCF-7 cells over-expressing Cx43 formed smaller sized clusters and Cx43 expressing MDA-MB-231 cells lost their stellar morphology. Extravasation ability of both MCF-7 and MDA-MB-231 cells was reduced by 60% and 30% respectively. On the other hand, silencing Cx43 in MCF10A cells, nonneoplastic human mammary cell line, increased proliferation in both 2D and 3D cultures, and disrupted acinar morphology. Although Cx43 over-expression did not affect total levels of ?-catenin, ?-catenin and ZO-2, it decreased nuclear levels of ?-catenin in 2D and 3D cultures of MCF-7 cells, and in 3D cultures of MDA-MB-231 cells. Cx43 associated at the membrane with ?-catenin, ?-catenin and ZO-2 in 2D and 3D cultures of MCF-7 cells, and only in 3D conditions in MDA-MB-231 cells. This study suggests that Cx43 exerts tumor suppressive effects in a context-dependent manner where GJ assembly with ?-catenin, ?-catenin and ZO-2 may be implicated in reducing growth rate, invasiveness, and, malignant phenotype of 2D and 3D cultures of MCF-7 cells, and 3D cultures of MDA-MB-231 cells, by sequestering ?-catenin away from nucleus. - Highlights: Cx43 over-expressing MCF-7 and MDA-MB-231 were grown in 2D and 3D cultures. Proliferation and growth morphology were affected in a context dependent manner. Extravasation ability of both MCF-7 and MDA-MB-231 cells was reduced. Cx43-mediated gap junction complex assembly correlated with observed changes. We propose that membranous Cx43 sequesters ?-catenin away from the nucleus.

Talhouk, Rabih S., E-mail: rtalhouk@aub.edu.lb [Department of Biology, Faculty of Arts and Sciences, American University of Beirut, P.O. Box 11-0236, Beirut (Lebanon); Fares, Mohamed-Bilal; Rahme, Gilbert J.; Hariri, Hanaa H.; Rayess, Tina; Dbouk, Hashem A.; Bazzoun, Dana; Al-Labban, Dania [Department of Biology, Faculty of Arts and Sciences, American University of Beirut, P.O. Box 11-0236, Beirut (Lebanon); El-Sabban, Marwan E., E-mail: me00@aub.edu.lb [Department of Anatomy, Cell Biology and Physiology, Faculty of Medicine, American University of Beirut, P.O. Box 11-0236, Beirut (Lebanon)

2013-12-10T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

NON-INVASIVE OPTICAL DETECTION OF EPITHELIAL CANCER USING OBLIQUE INCIDENCE DIFFUSE REFLECTANCE SPECTROSCOPY  

E-Print Network [OSTI]

-pigmented lesions...................... 50 25 Results of the testing dataset for the non-pigmented lesions ..................... 51 26 Absorption coefficient spectra of common nevi, dysplastic nevi and melanoma... of abnormal cells that can only be seen by 5 histologic analysis and not through the endoscope. The current recommendation for patients who have a stable diagnosis of negative for dysplasia, confirmed by two endoscopic biopsy surveillance procedures...

Garcia-Uribe, Alejandro

2010-01-16T23:59:59.000Z

262

Microstructured Hydrogen Fuel Cells  

Science Journals Connector (OSTI)

Micro fuel cells ; Polymer electrolyte membrane fuel cells ; Proton exchange membrane fuel cells ...

Luc G. Frechette

2014-05-01T23:59:59.000Z

263

Protective effects of pulmonary epithelial lining fluid on oxidative stress and DNA single-strand breaks caused by ultrafine carbon black, ferrous sulphate and organic extract of diesel exhaust particles  

SciTech Connect (OSTI)

Pulmonary epithelial lining fluid (ELF) is the first substance to make contact with inhaled particulate matter (PM) and interacts chemically with PM components. The objective of this study was to determine the role of ELF in oxidative stress, DNA damage and the production of proinflammatory cytokines following physicochemical exposure to PM. Ultrafine carbon black (ufCB, 15 nm; a model carbonaceous core), ferrous sulphate (FeSO{sub 4}; a model transition metal) and a diesel exhaust particle (DEP) extract (a model organic compound) were used to examine the acellular oxidative potential of synthetic ELF and non-ELF systems. We compared the effects of exposure to ufCB, FeSO{sub 4} and DEP extract on human alveolar epithelial Type II (A549) cells to determine the levels of oxidative stress, DNA single-strand breaks and interleukin-8 (IL-8) production in ELF and non-ELF systems. The effects of ufCB and FeSO{sub 4} on the acellular oxidative potential, cellular oxidative stress and DNA single-strand breakage were mitigated significantly by the addition of ELF, whereas there was no decrease following treatment with the DEP extract. There was no significant effect on IL-8 production following exposure to samples that were suspended in ELF/non-ELF systems. The results of the present study indicate that ELF plays an important role in the initial defence against PM in the pulmonary environment. Experimental components, such as ufCB and FeSO{sub 4}, induced the production of oxidative stress and led to DNA single-strand breaks, which were moderately prevented by the addition of ELF. These findings suggest that ELF plays a protective role against PM-driven oxidative stress and DNA damage. -- Highlights: ? To determine the role of ELF in ROS, DNA damage and IL-8 after exposure to PM. ? ufCB, FeSO{sub 4} and DEP extract were used to examine the protective effects of ELF. ? PM-driven oxidative stress and DNA single-strand breakage were mitigated by ELF. ? The findings suggest that ELF has a protective role against PM. ? The synthetic ELF system could reduce the use of animals in PM-driven ROS testing.

Chuang, Hsiao-Chi [School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan (China) [School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan (China); Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan (China); Cheng, Yi-Ling; Lei, Yu-Chen [Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan (China)] [Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan (China); Chang, Hui-Hsien [Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan (China)] [Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan (China); Cheng, Tsun-Jen, E-mail: tcheng@ntu.edu.tw [Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan (China) [Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan (China); Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan (China)

2013-02-01T23:59:59.000Z

264

Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cells Fuel Cells Converting chemical energy of hydrogenated fuels into electricity Project Description Invented in 1839, fuels cells powered the Gemini and Apollo space missions, as well as the space shuttle. Although fuel cells have been successfully used in such applications, they have proven difficult to make more cost-effective and durable for commercial applications, particularly for the rigors of daily transportation. Since the 1970s, scientists at Los Alamos have managed to make various scientific breakthroughs that have contributed to the development of modern fuel cell systems. Specific efforts include the following: * Finding alternative and more cost-effective catalysts than platinum. * Enhancing the durability of fuel cells by developing advanced materials and

265

Calcitriol inhibits Ether-a go-go potassium channel expression and cell proliferation in human breast cancer cells  

SciTech Connect (OSTI)

Antiproliferative actions of calcitriol have been shown to occur in many cell types; however, little is known regarding the molecular basis of this process in breast carcinoma. Ether-a-go-go (Eag1) potassium channels promote oncogenesis and are implicated in breast cancer cell proliferation. Since calcitriol displays antineoplastic effects while Eag1 promotes tumorigenesis, and both factors antagonically regulate cell cycle progression, we investigated a possible regulatory effect of calcitriol upon Eag1 as a mean to uncover new molecular events involved in the antiproliferative activity of this hormone in human breast tumor-derived cells. RT real-time PCR and immunocytochemistry showed that calcitriol suppressed Eag1 expression by a vitamin D receptor (VDR)-dependent mechanism. This effect was accompanied by inhibition of cell proliferation, which was potentiated by astemizole, a nonspecific Eag1 inhibitor. Immunohistochemistry and Western blot demonstrated that Eag1 and VDR abundance was higher in invasive-ductal carcinoma than in fibroadenoma, and immunoreactivity of both proteins was located in ductal epithelial cells. Our results provide evidence of a novel mechanism involved in the antiproliferative effects of calcitriol and highlight VDR as a cancer therapeutic target for breast cancer treatment and prevention.

Garcia-Becerra, Rocio [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Diaz, Lorenza, E-mail: lorenzadiaz@gmail.com [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Camacho, Javier [Department of Pharmacology, Centro de Investigacion y de Estudios Avanzados, Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco 07360, Mexico, D.F. (Mexico)] [Department of Pharmacology, Centro de Investigacion y de Estudios Avanzados, Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco 07360, Mexico, D.F. (Mexico); Barrera, David; Ordaz-Rosado, David; Morales, Angelica [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Ortiz, Cindy Sharon [Department of Pathology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Pathology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Avila, Euclides [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Bargallo, Enrique [Department of Breast Tumors, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico)] [Department of Breast Tumors, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico); Arrecillas, Myrna [Department of Pathology, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico)] [Department of Pathology, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico); Halhali, Ali; Larrea, Fernando [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)

2010-02-01T23:59:59.000Z

266

Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cells Fuel Cells The Solid State Energy Conversion Alliance (SECA) program is responsible for coordinating Federal efforts to facilitate development of a commercially relevant and robust solid oxide fuel cell (SOFC) system. Specific objectives include achieving an efficiency of greater than 60 percent, meeting a stack cost target of $175 per kW, and demonstrating lifetime performance degradation of less than 0.2 percent per

267

Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cells The Solid State Energy Conversion Alliance (SECA) program is responsible for coordinating Federal efforts to facilitate development of a commercially relevant and robust...

268

The Potent Carcinogen Dibenzo[a,l]pyrene Is Metabolically Activated to Fjord-Region 11,12-Diol 13,14-Epoxides in Human Mammary Carcinoma MCF-7 Cell Cultures  

Science Journals Connector (OSTI)

...Baird 3 Department of Medicinal Chemistry and Pharmacognosy, Purdue University...addressed, at Department of Medicinal Chemistry, Hansen Life Sciences Research...studies of emissions from unvented coal combustion. Environ. Sci. Technol., 21...

Sherry L. Ralston; Hudson H. S. Lau; Albrecht Seidel; Andreas Luch; Karl L. Platt; and William M. Baird

1994-02-15T23:59:59.000Z

269

Interspecies Differences in the Major DNA Adducts Formed from Benzo(a)pyrene but not 7,12-Dimethylbenz(a)anthracene in Rat and Human Mammary Cell Cultures  

Science Journals Connector (OSTI)

...hydrolysates were applied to Sep-Pak CIS car tridges (Waters Associates, Milford, MA). Unreacted...column was eluted with metha- nol:water (46:54) for 34 min at a flow...relation between carcinogenic power of hydrocarbons and their binding...

Cynthia J. Moore; Donna Pruess-Schwartz; Robert J. Mauthe; Michael N. Gould; and William M. Baird

1987-08-15T23:59:59.000Z

270

Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Materials Science » Materials Science » Fuel Cells Fuel Cells Research into alternative forms of energy, especially energy security, is one of the major national security imperatives of this century. Get Expertise Melissa Fox Applied Energy Email Catherine Padro Sensors & Electrochemical Devices Email Fernando Garzon Sensors & Electrochemical Devices Email Piotr Zelenay Sensors & Electrochemical Devices Email Rod Borup Sensors & Electrochemical Devices Email Karen E. Kippen Experimental Physical Sciences Email Like a battery, a fuel cell consists of two electrodes separated by an electrolyte-in polymer electrolyte fuel cells, the separator is made of a thin polymeric membrane. Unlike a battery, a fuel cell does not need recharging-it continues to produce electricity as long as fuel flows

271

Highly purified, multi-wall carbon nanotubes induce light-chain 3B expression in human lung cells  

SciTech Connect (OSTI)

Highlights: HTT2800-treated BEAS-2B cells induced LC3B in a time-dependent manner. HTT2800-treated BEAS-2B cells showed decreased cell proliferation that was both time- and dose-dependent. Addition of 3-MA, LC3B-II protein and mRNA levels were significantly decreased. 3-MA and E64-d + pepstatin A, but not brefeldin A, provided protection against HTT2800-induced cell death. These results suggest that HTT2800 predominantly causes autophagy rather than apoptotic cell death in BEAS-2B cells. -- Abstract: Bronchial epithelial cells are targets of inhalation and play a critical role in the maintenance of mucosal integrity as mechanical barriers against various particles. Our previous result suggest that vapor-grown carbon fiber, HTT2800, which is one of the most highly purified multi-wall carbon nanotubes (MWCNT) showed cellular uptake of the carbon nanotube, increased cell death, enhanced DNA damage, and induced cytokine release. Increasing evidence suggests that autophagy may critically influence vital cellular processes such as apoptosis, cell proliferation and inflammation and thereby may play a critical role in pulmonary diseases. Autophagy was recently recognized as a critical cell death pathway, and autophagosome accumulation has been found to be associated with the exposure of various nanoparticles. In this study, the authors focus on the autophagic responses of HTT2800 exposure. The HTT2800-exposed cells induced LC3B expression and induced cell growth inhibition.

Tsukahara, Tamotsu, E-mail: ttamotsu@kanazawa-med.ac.jp [Department of Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293 (Japan)] [Department of Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293 (Japan); Matsuda, Yoshikazu [Clinical Pharmacology Educational Center, Nihon Pharmaceutical University, Ina-machi, Saitama 362-0806 (Japan)] [Clinical Pharmacology Educational Center, Nihon Pharmaceutical University, Ina-machi, Saitama 362-0806 (Japan); Usui, Yuki [Research Center for Exotic Nanocarbons, Shinshu University, 4-17-1 Wakasato, Nagano-shi, Nagano 380-8553 (Japan)] [Research Center for Exotic Nanocarbons, Shinshu University, 4-17-1 Wakasato, Nagano-shi, Nagano 380-8553 (Japan); Haniu, Hisao [Department of Orthopaedic Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621 (Japan)] [Department of Orthopaedic Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621 (Japan)

2013-10-18T23:59:59.000Z

272

E-Print Network 3.0 - acid inhibits tumor Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

we show here that intrametastatic lymphatic vessels and bulk tumor cell... model of tumor bulk invasion, human mammary carcinoma cells caused ... Source: Floreano, Dario -...

273

Endoglin (CD105) Contributes to Platinum Resistance and Is A Target for Tumor-Specific Therapy in Epithelial Ovarian Cancer  

Science Journals Connector (OSTI)

...Objectives. 293T was genetically modified to secrete tissue inhibitor of metalloproteinase-2 (TIMP-2) (abbreviated as 293TIMP2...cell viability, alginate microcapsules were reinforced with chitosan (0.1 % wt/vol). Expression of TIMP-2 protein in cell...

Angela J. Ziebarth; Somaira Nowsheen; Adam D. Steg; Monjri M. Shah; Ashwini A. Katre; Zachary C. Dobbin; Hee-Dong Han; Gabriel Lopez-Berestein; Anil K. Sood; Michael Conner; Eddy S. Yang; and Charles N. Landen

2013-01-01T23:59:59.000Z

274

Microfluidic Device for Studying Tumor Cell Extravasation in Cancer Metastasis  

SciTech Connect (OSTI)

Metastasis is the process by which cancer spreads to form secondary tumors at downstream locations throughout the body. This uncontrolled spreading is the leading cause of death in patients with epithelial cancers and is the main reason that suppressing and targeting cancer has proven to be so challenging. Tumor cell extravasation is one of the key steps in cancer s progression towards a metastatic state. This occurs when circulating tumor cells found within the blood stream are able to transmigrate through the endothelium lining and basement membrane of the vasculature to form metastatic tumors at secondary sites within the body. Predicting the likelihood of this occurrence in patients, or being able to determine specific markers involved in this process could lead to preventative measures targeting these types of cancer; moreover, this may lead to the discovery of novel anti-metastatic drugs. We have developed a microfluidic device that has shown the extravasation of fluorescently labeled tumor cells across an endothelial cell lined membrane coated with matrigel followed by the formation of colonies. This device provides the advantages of combining a controlled environment, mimicking that found within the body, with real-time monitoring capabilities allowing for the study of these biomarkers and cellular interactions along with other potential mechanisms involved in the process of extravasation.

Lin, Henry K [ORNL; Thundat, Thomas George [ORNL; Evans III, Boyd Mccutchen [ORNL; Datar, Ram H [ORNL; Reese, Benjamin E [ORNL; Zheng, Siyang [ORNL

2010-01-01T23:59:59.000Z

275

Laryngeal Papilloma Cells Have High Levels of Epidermal Growth Factor Receptor and Respond to Epidermal Growth Factor by a Decrease in Epithelial Differentiation  

Science Journals Connector (OSTI)

...were used in these experiments (33). The positive control was pPPl, generously provided by Dr. Joseph Sambrook. It contains the...that served as a negative control, the intact SV40 enhancer (pPPl ) as a positive control, and the HPV 11 upstream regulatory...

Andrea Vambutas; Teresa P. Di Lorenzo; Bettie M. Steinberg

1993-02-15T23:59:59.000Z

276

Abstract 3699: Multi-walled carbon nanotubes induce apoptosis in normal human small airway epithelial cells through proteasome-mediated Mcl-1 protein degradation  

Science Journals Connector (OSTI)

...Washington, DC Abstract 3699: Multi-walled carbon nanotubes induce...are emerging as the major building blocks in nanotechnology thanks...CNTs, including single- and multi-walled (SWCNTs and MWCNT...the anti-apoptotic Bcl-2 family proteins and found that only...

Bao-Zhu Yuan; Joshua Chapman; Yon Rojanasakul; Vincent Castranova; and Steven H. Reynolds

2011-01-10T23:59:59.000Z

277

Identification of a Gene Containing Zinc-Finger Motifs Based on Lost Expression in Malignantly Transformed Rat Ovarian Surface Epithelial Cells  

Science Journals Connector (OSTI)

...pattern of certain genes, such as p73 and WTI. Here, we have shown that it appears that...and Oshimura, M. Patemal expression of WTI in human fibroblasts and lymphocytes. Hum...Mosaic and polymorphic imprinting of the WTI gene in humans. Nat. Genet., 6: 305-309...

Abbas Abdollahi; Andrew K. Godwin; Paul D. Miller; Lori A. Getts; David C. Schultz; Takahiro Taguchi; Joseph R. Testa; Thomas C. Hamilton

1997-05-15T23:59:59.000Z

278

The Adhesion AND Growth of Both the Human Primary Gingival Epithelial Cells and Streptococcus Mutans on Micro-Arc Oxidized Titanium  

Science Journals Connector (OSTI)

With good osseointegration properties, micro-arc oxidation has now gradually become the key point ... but interface between the implant surface treated by micro-arc oxidation and gingival soft tissues has been se...

Jiangning Lv; Hongwei Li; Ying Mu; ShuMin Wang

2014-11-01T23:59:59.000Z

279

Abstract 1498: Alpha-Lipoic acid suppresses migration and invasiveness of thyroid cancer cells through inhibition of epithelial-mesenchymal transition .  

Science Journals Connector (OSTI)

...2 Ji Hye Yim 1 Won Gu Kim 1 Tae Yong Kim 1 Young Kee Shong 1 Eui Young Kim 3 Won Bae Kim 1 1Asan Medical Center, Seoul, Republic...Ji Hye Yim, Won Gu Kim, Tae Yong Kim, Young Kee Shong, Eui Young Kim, Won Bae Kim. Alpha-Lipoic acid suppresses migration...

Ji Min Han; Hyun-Jeung Choi; Ji Hye Yim; Won Gu Kim; Tae Yong Kim; Young Kee Shong; Eui Young Kim; Won Bae Kim

2013-04-15T23:59:59.000Z

280

Differences in Repair of DNA Cross-links between Lymphocytes and Epithelial Tumor Cells from Colon Cancer Patients Measured In vitro with the Comet Assay  

Science Journals Connector (OSTI)

...status at the last contact date). We used t-test, Kruskal-Wallis test and linear regression to explore the relationship...significant difference (global P=0.061 from Kruskal-Wallis test) by tumor size in the three smoker groups...

Mercedes Herrera; Gemma Dominguez; Jose M. Garcia; Cristina Pea; Carmen Jimenez; Javier Silva; Vanesa Garcia; Irene Gomez; Raquel Diaz; Paloma Martin; Felix Bonilla

2009-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Differences in Repair of DNA Cross-links between Lymphocytes and Epithelial Tumor Cells from Colon Cancer Patients Measured In vitro with the Comet Assay  

Science Journals Connector (OSTI)

...olive tail moment. Results: Kruskal-Wallis analysis revealed significant...Pearson correlation coefficient and Kruskal-Wallis analysis. In all statistical...comet parameters used indicates. Kruskal-Wallis analysis showed not only significant...

Mercedes Herrera; Gemma Dominguez; Jose M. Garcia; Cristina Pea; Carmen Jimenez; Javier Silva; Vanesa Garcia; Irene Gomez; Raquel Diaz; Paloma Martin; Felix Bonilla

2009-09-01T23:59:59.000Z

282

Transmembrane protein PERP is a component of tessellate junctions and of other junctional and non-junctional plasma membrane regions in diverse epithelial and epithelium-derived cells  

E-Print Network [OSTI]

Protein PERP (p53 apoptosis effector related to PMP-22) is a small (21.4 kDa) transmembrane polypeptide with an amino acid sequence indicative of a tetraspanin character. It is enriched in the plasma membrane and apparently ...

Franke, Werner W.

283

Induction of the Stellate Configuration in Cultured Iris Epithelial Cells by Adenosine and Compounds Related to Adenosine 3?:5?-Cyclic Monophosphate  

Science Journals Connector (OSTI)

...Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830 Communicated...tion of adenosine found by those workers coincides with that found in the...Puerto Rico, Rio Piedras, P.R. Oak Ridge National Laboratory is operated by...

Jose R. Ortiz; Tuneo Yamada; Abraham W. Hsie

1973-01-01T23:59:59.000Z

284

Plastin3 Is a Novel Marker for Circulating Tumor Cells Undergoing the EpithelialMesenchymal Transition and Is Associated with Colorectal Cancer Prognosis  

Science Journals Connector (OSTI)

...21679006; and NEDO (New Energy and Industrial Technology...in cancer patients: challenges and perspectives.Trends...to routine diagnostic implementation?Eur J Cancer 2007...13:920-8. 7. Wind J , Tuynman JB, Tibbe...Pantel K.Biologic Challenges in the detection of...

Takehiko Yokobori; Hisae Iinuma; Teppei Shimamura; Seiya Imoto; Keishi Sugimachi; Hideshi Ishii; Masaaki Iwatsuki; Daisuke Ota; Masahisa Ohkuma; Takeshi Iwaya; Naohiro Nishida; Ryunosuke Kogo; Tomoya Sudo; Fumiaki Tanaka; Kohei Shibata; Hiroyuki Toh; Tetsuya Sato; Graham F. Barnard; Takeo Fukagawa; Seiichiro Yamamoto; Hayao Nakanishi; Shin Sasaki; Satoru Miyano; Toshiaki Watanabe; Hiroyuki Kuwano; Koshi Mimori; Klaus Pantel; and Masaki Mori

2013-04-01T23:59:59.000Z

285

In vitro Toxicity of A-431 Carcinoma Cells with Antibodies to Epidermal Growth Factor Receptor and Epithelial Glycoprotein-1 Conjugated to Radionuclides Emitting Low-Energy Electrons  

Science Journals Connector (OSTI)

...anti-epidermal growth factor receptor and...which emit low-energy Auger and conversion electrons. Conjugates...emitting low-energy electrons to...anti-epidermal growth factor receptor and...which emit low-energy Auger and conversion electrons. Conjugates...

Rosana B. Michel; Mary Ellen Castillo; Philip M. Andrews; and M. Jules Mattes

2004-09-01T23:59:59.000Z

286

Epstein-Barr Virus in a Malignant Lymphoproliferative Disorder of B-Cells Occurring after Thymic Epithelial Transplantation for Combined Immunodeficiency  

Science Journals Connector (OSTI)

...Nucleic Acid Hybridization Thymus Gland microbiology pathology transplantation Thymus Hormones...Justine Hospital and the Department of Microbiology and Immunology, University of Montreal...Hong. R., Horowitz, S. D., Gilbert, E., Kaufman, D., De- Mendonca...

Elena R. Reece; John G. Gartner; Thomas A. Seemayer; Jean H. Joncas; and Joseph S. Pagano

1981-11-01T23:59:59.000Z

287

Retinoic acid receptor- and retinoid X receptor-selective retinoids activate signaling pathways that converge on AP-1 and inhibit squamous differentiation in human bronchial epithelial cells  

Science Journals Connector (OSTI)

...H. B., M. F.], and SRI International, Menlo Park, California 94025...F.. H. B., M. FJ. and SRI International. Mento Park, Cali/arnia 94025...8-telramethyl-2-naphthamido)benzoic acid; SRI 1235. [2-15.6.7,8...

HY Lee; MI Dawson; GL Walsh; JC Nesbitt; RL Eckert; E Fuchs; WK Hong; R Lotan; and JM Kurie

1996-08-01T23:59:59.000Z

288

Smoking Induces Epithelial-to-Mesenchymal Transition in NonSmall Cell Lung Cancer through HDAC-Mediated Downregulation of E-Cadherin  

Science Journals Connector (OSTI)

...HDAC-Mediated Downregulation of E-Cadherin Nagaraj S. Nagathihalli 1 2 6 Pierre P. Massion 3 6 Adriana L. Gonzalez 4 Pengcheng Lu 5 Pran K. Datta 1 6 7 8 9 Corresponding Author: Pran K. Datta, Department of Medicine, University of Alabama at Birmingham...

Nagaraj S. Nagathihalli; Pierre P. Massion; Adriana L. Gonzalez; Pengcheng Lu; Pran K. Datta

2012-11-01T23:59:59.000Z

289

Evaluation of Epithelial Cell Proliferation Rate in Normal-appearing Colonic Mucosa as a High-Risk Marker for Colorectal Cancer  

Science Journals Connector (OSTI)

...Horii, Osaka, Japan). All of the patients...min at 500 W in a household microwave oven...Corp., Tokyo, Japan) as a primary antibody...patients who had higher energy intake had significantly...case-control study in Japan. Cancer Causes Control...Edwards S. L. Dietary energy sources and colon...

Ikuko Akedo; Hideki Ishikawa; Tatsuya Ioka; Itaru Kaji; Hiroyuki Narahara; Shingo Ishiguro; Takaichiro Suzuki; and Toru Otani

2001-09-01T23:59:59.000Z

290

Abstract #2266: Cables 1 knockdown in immortalized human ovarian surface epithelial cells results in their uptake of more malignant like characteristics  

Science Journals Connector (OSTI)

...2009; Denver, CO Abstract #2266: Cables 1 knockdown in immortalized human ovarian...Meeting-- Apr 18-22, 2009; Denver, CO Cables 1 is best known as a cyclin-dependent...Previously we reported that loss of Cables 1 expression was observed with high frequency...

Hideo Sakamoto; Anne Friel; Lawrence Zukerberg; Ronny Drapkin; Bo Rueda

2009-05-01T23:59:59.000Z

291

Electrochemical cell  

DOE Patents [OSTI]

An improved secondary electrochemical cell is disclosed having a negative electrode of lithium aluminum, a positive electrode of iron sulfide, a molten electrolyte of lithium chloride and potassium chloride, and the combination that the fully charged theoretical capacity of the negative electrode is in the range of 0.5-1.0 that of the positive electrode. The cell thus is negative electrode limiting during discharge cycling. Preferably, the negative electrode contains therein, in the approximate range of 1-10 volume % of the electrode, an additive from the materials of graphitized carbon, aluminum-iron alloy, and/or magnesium oxide.

Kaun, Thomas D. (New Lenox, IL)

1984-01-01T23:59:59.000Z

292

Development of a combined model of tissue kinetics and radiation response of human bronchiolar epithelium with single cell resolution  

E-Print Network [OSTI]

cells of the airways due to internal exposure to alpha-particle emitters, e.g. radon. Inhalation of radon, a colorless and odorless gas, one of the products of the decay of uranium which occurs naturally in the earth?s crust, is the second major cause... epithelial tissue plays an important role in normal lung physiology. square4 lung epithelia are target tissues for occupational internal exposures and for radon exposure (26); square4 the epithelium of bronchioles appears to be the origin...

Ostrovskaya, Natela Grigoryevna

2006-10-30T23:59:59.000Z

293

Disruption of canonical TGF?-signaling in murine coronary progenitor cells by low level arsenic  

SciTech Connect (OSTI)

Exposure to arsenic results in several types of cancers as well as heart disease. A major contributor to ischemic heart pathologies is coronary artery disease, however the influences by environmental arsenic in this disease process are not known. Similarly, the impact of toxicants on blood vessel formation and function during development has not been studied. During embryogenesis, the epicardium undergoes proliferation, migration, and differentiation into several cardiac cell types including smooth muscle cells which contribute to the coronary vessels. The TGF? family of ligands and receptors is essential for developmental cardiac epithelial to mesenchymal transition (EMT) and differentiation into coronary smooth muscle cells. In this in vitro study, 18 hour exposure to 1.34 ?M arsenite disrupted developmental EMT programming in murine epicardial cells causing a deficit in cardiac mesenchyme. The expression of EMT genes including TGF?2, TGF? receptor-3, Snail, and Has-2 are decreased in a dose-dependent manner following exposure to arsenite. TGF?2 cell signaling is abrogated as detected by decreases in phosphorylated Smad2/3 when cells are exposed to 1.34 ?M arsenite. There is also loss of nuclear accumulation pSmad due to arsenite exposure. These observations coincide with a decrease in vimentin positive mesenchymal cells invading three-dimensional collagen gels. However, arsenite does not block TGF?2 mediated smooth muscle cell differentiation by epicardial cells. Overall these results show that arsenic exposure blocks developmental EMT gene programming in murine coronary progenitor cells by disrupting TGF?2 signals and Smad activation, and that smooth muscle cell differentiation is refractory to this arsenic toxicity. - Highlights: Arsenic blocks TGF?2 induced expression of EMT genes. Arsenic blocks TGF?2 triggered Smad2/3 phosphorylation and nuclear translocation. Arsenic blocks epicardial cell differentiation into cardiac mesenchyme. Arsenic does not block TGF?2 induced smooth muscle cell differentiation.

Allison, Patrick; Huang, Tianfang; Broka, Derrick; Parker, Patti [Department of Pharmacology and Toxicology College of Pharmacy, Southwest Environmental Health Sciences Center, Steele Children's Research Center and Bio5 Institute, University of Arizona, Tucson, AZ 85721 (United States); Barnett, Joey V. [Department of Pharmacology, Vanderbilt Medical University, Nashville, TN (United States); Camenisch, Todd D., E-mail: camenisch@pharmacy.arizona.edu [Department of Pharmacology and Toxicology College of Pharmacy, Southwest Environmental Health Sciences Center, Steele Children's Research Center and Bio5 Institute, University of Arizona, Tucson, AZ 85721 (United States)

2013-10-01T23:59:59.000Z

294

Photovoltaic cell  

DOE Patents [OSTI]

In a photovoltaic cell structure containing a visibly transparent, electrically conductive first layer of metal oxide, and a light-absorbing semiconductive photovoltaic second layer, the improvement comprising a thin layer of transition metal nitride, carbide or boride interposed between said first and second layers.

Gordon, Roy G. (Cambridge, MA); Kurtz, Sarah (Somerville, MA)

1984-11-27T23:59:59.000Z

295

Nanocrystal Solar Cells  

E-Print Network [OSTI]

research on organic photovoltaic cells since small molecule10 years prior (4). Photovoltaic cells with an active layerof the associated photovoltaic cells. 2.4 Charge transport

Gur, Ilan

2006-01-01T23:59:59.000Z

296

Epithelial-Mesenchymal Interactions  

Science Journals Connector (OSTI)

...eliminate labor and cleaning equipment costs * Let you use new cage for each experiment...Lexington Ave., New York 10017) 4-6. Nuclear Mletallutrgy Conf.. Phoenix. Ariz...Ave.. Chicago. 111. 60611) 30-2. Nuclear Science. 14th symp., Los Angeles...

Raul Fleischmajer

1967-09-22T23:59:59.000Z

297

Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities Presentation covers stationary fuel cells...

298

Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar | Department...  

Broader source: Energy.gov (indexed) [DOE]

Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar Presentation by Sunita Satyapal at the Fuel Cell Seminar on November...

299

Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar | Department...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Overview: 2011 Fuel Cell Seminar Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar Presentation by Sunita Satyapal at the Fuel Cell Seminar on November 1, 2011. Fuel Cell...

300

Electrorefining cell evaluation  

SciTech Connect (OSTI)

Operational characteristics of the LANL electrorefining cell, a modified LANL electrorefining cell, and an advanced electrorefining cell (known as the CRAC cell) were determined. Average process yields achieved were: 75% for the LANL cell, 82% for the modified LANL cell, and 86% for the CRAC cell. All product metal from the LANL and modified LANL cells was within foundry specifications. Metal from one run in the CRAC cell exceeded foundry specifications for tantalum. The LANL and modified LANL cells were simple in design and operation, but product separation was more labor intensive than with the CRAC cell. The CRAC cell was more complicated in design but remained relatively simple in operation. A decision analysis concluded that the modified LANL cell was the preferred cell. It was recommended that the modified LANL cell be implemented by the Plutonium Recovery Project at Rocky Flats and that development of the CRAC cell continue. 8 refs., 22 figs., 12 tabs.

Bronson, M.C.; Thomas, R.L. (ed.)

1989-04-14T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Effects of the PPAR{gamma} agonist troglitazone on endothelial cells in vivo and in vitro: Differences between human and mouse  

SciTech Connect (OSTI)

Peroxisome proliferator-activated receptor gamma (PPAR{gamma}) agonists and PPAR{gamma}/{alpha} dual agonists have been or are being developed for clinical use in the treatment of type 2 diabetes mellitus and hyperlipidemias. A common tumor finding in rodent carcinogenicity studies for these agonists is hemangioma/hemangiosarcoma in mice but not in rats. We hypothesized that increased endothelial cell proliferation may be involved in the mechanism of PPAR agonist-induced vascular tumors in mice, and we investigated the effects on endothelial cells utilizing troglitazone, the first clinically used PPAR{gamma} agonist, in vivo and in vitro. Troglitazone (400 and 800 mg/kg/day) induced hemangiosarcomas in mice in a 2-year bioassay. We showed that troglitazone increased endothelial cell proliferation in brown and white adipose tissue and liver in mice at sarcomagenic doses after 4 weeks of treatment. Troglitazone was cytotoxic both to human dermal microvascular endothelial cells (HMEC1) and mouse mammary fat pad microvascular endothelial cells (MFP MVEC) at high concentrations. However, MFP MVEC were more resistant to the cytotoxic effects of troglitazone based on the much lower LC{sub 50} in HMEC1 (17.4 {mu}M) compared to MFP MVEC (92.2 {mu}M). Troglitazone increased the proliferation and survival of MFP MVEC but not HMEC1 in growth factor reduced conditions. Our data demonstrate that troglitazone may induce hemangiosarcomas in mice, at least in part, through enhancement of survival and proliferation of microvascular endothelial cells. Such an effect does not occur with human cells, suggesting that human may react differently to exposure to PPAR agonists compared with mice.

Kakiuchi-Kiyota, Satoko [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States); Vetro, Joseph A. [Center for Drug Delivery and Nanomedicine and Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198-6025 (United States); Suzuki, Shugo; Varney, Michelle L. [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States); Han, Huai-Yun [Center for Drug Delivery and Nanomedicine and Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198-6025 (United States); Nascimento, Merielen; Pennington, Karen L.; Arnold, Lora L.; Singh, Rakesh K. [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States); Cohen, Samuel M. [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States)], E-mail: scohen@unmc.edu

2009-05-15T23:59:59.000Z

302

Electrochemistry Cell Model  

Broader source: Energy.gov (indexed) [DOE]

or exceeds all performance goals - Interpreting complex cell electrochemical phenomena - Identification of cell degradation mechanisms Partners (Collaborators) Daniel Abraham,...

303

Binding of ETS family members is important for the function of the c-sis/platelet-derived growth factor-B TATA neighboring sequence in 12-O-tetradecanoylphorbol-13-acetate-treated K562 cells  

Science Journals Connector (OSTI)

...Spontaneous Mammary Carcinomas of BALB/c Mice and in the Responsiveness of the...Immunization of young adult BALB/c mice, mammary tumor virus (MTV...234-238, 1962. 22. Martinez, C., Shapiro, F., and Good, R. A. Induction...

GC Kujoth; DF Robinson; and WE Fahl

1998-07-01T23:59:59.000Z

304

Excitonic Solar Cells  

Science Journals Connector (OSTI)

Excitonic Solar Cells ... Existing types of solar cells may be divided into two distinct classes:? conventional solar cells, such as silicon p?n junctions, and excitonic solar cells, XSCs. ... Most organic-based solar cells, including dye-sensitized solar cells, DSSCs, fall into the category of XSCs. ...

Brian A. Gregg

2003-05-01T23:59:59.000Z

305

Telecommunications International Cell Phone  

E-Print Network [OSTI]

Telecommunications International Cell Phone 1. Fax completed form to 979.847.1111. 2. If you do will be charged. Date Cell Phone Needed Cell Phone Pick-Up Date Cell Phone User Travel Destination(s) United States Number Destination Country Number Cell Phone Type Digital Satellite Cell Phone Return Date Notes

306

Defining the Role of Histone Deacetylases in the Inhibition of Mammary Carcinogenesis by Dietary Energy Restriction (DER): Effects of Suberoylanilide Hydroxamic Acid (SAHA) and DER in a Rat Model  

Science Journals Connector (OSTI)

...stress and DER is a recognized energy stressor. Therefore, it was...deletion of transformed cells and sustainable protection against some aspects...cellular function including energy metabolism with a particularly...the NAD/NADH ratio and the energy charge of the cell, although...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; and Henry J. Thompson

2013-04-01T23:59:59.000Z

307

Origins and consequences of radiation–induced centrosome aberrations  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Origins and consequences of radiation–induced centrosome aberrations Origins and consequences of radiation–induced centrosome aberrations Sangeetha Vijayakumar New York University School of Medicine Abstract Centrosome aberrations are frequently observed in pre-neoplastic breast lesions and are known to drive chromosomal instability (Lingle et al., 2002). Previous studies from our lab have shown that human mammary epithelial cells exposed to low doses of radiation exhibit centrosome aberrations (CAs) in a dose dependent manner from 10-200 cGy (Maxwell et al., 2008). These data demonstrated that radiation-induced CAs actually precede and generate genomic instability and that TGFβ is a key mediator of genomic surveillance by eliminating genomically unstable cells through p53-dependent apoptosis. While high dose radiation has been shown to cause centrosome aberrations

308

Three Human Cell Types Respond to Multi-Walled Carbon Nanotubes and Titanium Dioxide Nanobelts with Cell-Specific Transcriptomic and Proteomic Expression Patterns.  

SciTech Connect (OSTI)

The growing use of engineered nanoparticles (NPs) in commercial and medical applications raises the urgent need for tools that can predict NP toxicity. Global transcriptome and proteome analyses were conducted on three human cell types, exposed to two high aspect ratio NP types, to identify patterns of expression that might indicate high versus low NP toxicity. Three cell types representing the most common routes of human exposure to NPs, including macrophage-like (THP-1), small airway epithelial and intestinal (Caco-2/HT29-MTX) cells, were exposed to TiO2 nanobelts (TiO2-NB; high toxicity) and multi-walled carbon nanotubes (MWCNT; low toxicity) at low (10 g/mL) and high (100 g/mL) concentrations for 1 and 24 h. Unique patterns of gene and protein expressions were identified for each cell type, with no differentially expressed (p < 0.05, 1.5-fold change) genes or proteins overlapping across all three cell types. While unique to each cell type, the early response was primarily independent of NP type, showing similar expression patterns in response to both TiO2-NB and MWCNT. The early response might, therefore, indicate a general response to insult. In contrast, the 24 h response was unique to each NP type. The most significantly (p < 0.05) enriched biological processes in THP-1 cells indicated TiO2-NB regulation of pathways associated with inflammation, apoptosis, cell cycle arrest, DNA replication stress and genomic instability, while MWCNT-regulated pathways indicated increased cell proliferation, DNA repair and anti-apoptosis. These two distinct sets of biological pathways might, therefore, underlie cellular responses to high and low NP toxicity, respectively.

Tilton, Susan C.; Karin, Norman J.; Tolic, Ana; Xie, Yumei; Lai, Xianyin; Hamilton, Raymond F.; Waters, Katrina M.; Holian, Andrij; Witzmann, Frank A.; Orr, Galya

2014-08-01T23:59:59.000Z

309

Photoelectrochemical cell  

DOE Patents [OSTI]

A photoelectrochemical cell comprising a sealed container having a light-transmitting window for admitting light into the container across a light-admitting plane, an electrolyte in the container, a photoelectrode in the container having a light-absorbing surface arranged to receive light from the window and in contact with the electrolyte, the surface having a plurality of spaced portions oblique to the plane, each portion having dimensions at least an order of magnitude larger than the maximum wavelength of incident sunlight, the total surface area of the surface being larger than the area of the plane bounded by the container, and a counter electrode in the container in contact with the electrolyte.

Rauh, R. David (Newton, MA); Boudreau, Robert A. (Norton, MA)

1983-06-14T23:59:59.000Z

310

Disturbance of DKK1 level is partly involved in survival of lung cancer cells via regulation of ROMO1 and ?-radiation sensitivity  

SciTech Connect (OSTI)

Highlights: DKK1 was expressed differently among non-small-cell lung cancer cell lines. DKK1 negatively regulated ROMO1 gene expression. Disturbance of DKK1 level induced the imbalance of cellular ROS. DKK1/ROMO1-induced ROS imbalance is involved in cell survival in NSCLC. -- Abstract: Dickkopf1 (DKK1), a secreted protein involved in embryonic development, is a potent inhibitor of the Wnt signaling pathway and has been postulated to be a tumor suppressor or tumor promoter depending on the tumor type. In this study, we showed that DKK1 was expressed differently among non-small-cell lung cancer cell lines. The DKK1 expression level was much higher in A549 cells than in H460 cells. We revealed that blockage of DKK1 expression by silencing RNA in A549 cells caused up-regulation of intracellular reactive oxygen species (ROS) modulator (ROMO1) protein, followed by partial cell death, cell growth inhibition, and loss of epithelialmesenchymal transition property caused by ROS, and it also increased ?-radiation sensitivity. DKK1 overexpression in H460 significantly inhibited cell survival with the decrease of ROMO1 level, which induced the decrease of cellular ROS. Thereafter, exogenous N-acetylcysteine, an antioxidant, or hydrogen peroxide, a pro-oxidant, partially rescued cells from death and growth inhibition. In each cell line, both overexpression and blockage of DKK1 not only elevated p-RB activation, which led to cell growth arrest, but also inactivated AKT/NF-kB, which increased radiation sensitivity and inhibited cell growth. This study is the first to demonstrate that strict modulation of DKK1 expression in different cell types partially maintains cell survival via tight regulation of the ROS-producing ROMO1 and radiation resistance.

Kim, In Gyu, E-mail: igkim@kaeri.re.kr [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Department of Radiation Biotechnology and Applied Radioisotope, University of Science and Technology (UST), 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Kim, Seo Yoen [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of) [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of); Kim, Hyun A; Kim, Jeong Yul [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of)] [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Lee, Jae Ha; Choi, Soo Im [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of) [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Department of Radiation Biotechnology and Applied Radioisotope, University of Science and Technology (UST), 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Han, Jeong Ran; Kim, Kug Chan [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of)] [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Cho, Eun Wie [Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of)] [Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of)

2014-01-03T23:59:59.000Z

311

Developmental time rather than local environment regulates the schedule of epithelial polarization in the zebrafish neural rod  

E-Print Network [OSTI]

within an initially solid neural rod primor- dium. Assembly of junctional complexes and polarity proteins at the midline marks the initiation of the colum- nar neuroepithelial architecture that is characteristic of vertebrate neural tubes. The emergence... information with the extrinsic envir- onment provides specificity of cell behavior in different tissues. Alternatively, an egg-timer-like mechanism may op- erate that consists of an intracellular factor that gradually increases or decreases in concentration...

Girdler, Gemma C; Araya, Claudio; Ren, Xiaoyun; Clarke, Jonathan DW

2013-03-24T23:59:59.000Z

312

The first cell sorter  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The first cell sorter The first cell sorter 1663 Los Alamos science and technology magazine Latest Issue:November 2013 All Issues » submit The first cell sorter About fifty years ago, a Los Alamos scientist invented a method-still important in cellular biology labs today-to separate out particular types of cells. November 25, 2013 The first cell sorter Flow cytometry (cell measurement) uses cell sorting to divert cells of a chosen type out of a mixed stream of cells, like the blood cells shown here, for collection and study. Los Alamos invented, and has regularly improved upon, the technology to isolate different kinds of cells. In the early-mid 1960s, Los Alamos physicist Mack Fulwyler invented a device to isolate different types of cells. His invention, still a vital aspect of flow cytometry (cell measurement) in biological laboratories

313

Fuel cell arrangement  

DOE Patents [OSTI]

A fuel cell arrangement is provided wherein cylindrical cells of the solid oxide electrolyte type are arranged in planar arrays where the cells within a plane are parallel. Planes of cells are stacked with cells of adjacent planes perpendicular to one another. Air is provided to the interior of the cells through feed tubes which pass through a preheat chamber. Fuel is provided to the fuel cells through a channel in the center of the cell stack; the fuel then passes the exterior of the cells and combines with the oxygen-depleted air in the preheat chamber.

Isenberg, Arnold O. (Forest Hills Boro, PA)

1987-05-12T23:59:59.000Z

314

Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar...  

Broader source: Energy.gov (indexed) [DOE]

Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar and Exposition Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar and Exposition Presentation by...

315

DOE Fuel Cell Technologies Office: 2013 Fuel Cell Seminar and...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

DOE Fuel Cell Technologies Office: 2013 Fuel Cell Seminar and Energy Exposition DOE Fuel Cell Technologies Office: 2013 Fuel Cell Seminar and Energy Exposition Overview of DOE's...

316

DOE Fuel Cell Technologies Office Record 13012: Fuel Cell System...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cell Technologies Office Record 13012: Fuel Cell System Cost - 2013 DOE Fuel Cell Technologies Office Record 13012: Fuel Cell System Cost - 2013 This program record from the...

317

In Vivo Imaging of Mesenchymal?Epithelial Transition Factor (c-Met) Expression using an Optical Imaging System  

Science Journals Connector (OSTI)

The flow was 1 mL/min, with the mobile phase varying from 97% solvent A (0.1% trifluoroacetic acid in water) and 3% solvent B (0.1% trifluoroacetic acid in acetonitrile; 0?4 min) to 40% solvent B for 10 min, 50% solvent B for 15 min, 40% solvent B for 10 min, and 90% solvent B for 25 min. ... The IC50 values using U87MG cells of cMBP-GGG-SC, and cMBP-AOC-C were 65 and 85.3 nM, respectively. ...

Eun-Mi Kim; Eun-Hye Park; Su-Jin Cheong; Chang-Moon Lee; Hwan-Jeong Jeong; Dong Wook Kim; Seok Tae Lim; Myung-Hee Sohn

2009-06-17T23:59:59.000Z

318

Fuel Cell Links  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cell Links Fuel Cell Links The links below are provided as additional resources for fuel-cell-related information. Most of the linked sites are not part of, nor affiliated with, fueleconomy.gov. We do not endorse or vouch for the accuracy of the information found on such sites. Fuel Cell Vehicles and Manufacturers Chevrolet General Motors press release about the Chevrolet Fuel Cell Equinox Ford Ford overview of their hydrogen fuel cell vehicles Honda FCX Clarity official site Hyundai Hyundai press release announcing the upcoming Tucson Fuel Cell Mercedes-Benz Ener-G-Force Fuel-cell-powered concept SUV Nissan Nissan TeRRA concept SUV Toyota Overview of Toyota fuel cell technology Hydrogen- and Fuel-Cell-Related Information and Tools Fuel Cell Vehicles Brief overview of fuel cell vehicles provided by DOE's Alternative Fuels Data Center (AFDC)

319

Photoelectrochemical solar cells  

Science Journals Connector (OSTI)

Photoelectrochemical solar cells ... Plastic Solar Cells: A Multidisciplinary Field To Construct Chemical Concepts from Current Research ... Plastic Solar Cells: A Multidisciplinary Field To Construct Chemical Concepts from Current Research ...

John T. McDevitt

1984-01-01T23:59:59.000Z

320

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

the membrane for a PEM fuel cell would cost $5/ft (1990$) inmass-produced PEM fuel cell could cost $10/kW or less. Totalparameter for PEM fuel cells: thinner membranes cost less

Delucchi, Mark

1992-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Nanocrystal Solar Cells  

E-Print Network [OSTI]

Nov, 2005). Chapter 4 Hybrid solar cells with 3-dimensional5 All-inorganic nanocrystal solar cells 5.1 Introduction Inoperation of organic based solar cells and distinguish them

Gur, Ilan

2006-01-01T23:59:59.000Z

322

Nanocrystal Solar Cells  

E-Print Network [OSTI]

inorganic nanocrystal solar cells 5.1 Introduction In recentoperation of organic based solar cells and distinguish themThe organic donor-acceptor solar cell relies on a type II

Gur, Ilan

2006-01-01T23:59:59.000Z

323

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

$ b materials cost, % a Fuel cell stack cost only. Includesof the cost of fuel-cell stacks, 1990$ Cost item GE Swan cAnnual maintenance cost of fuel cell stack and auxiliaries (

Delucchi, Mark

1992-01-01T23:59:59.000Z

324

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

Hydrogen Fuel Cell Vehicles UCD-ITS-RR-92-14 September byet al. , 1988,1989 HYDROGEN FUEL-CELL VEHICLES: TECHNICALIn the FCEV, the hydrogen fuel cell could supply the "net"

Delucchi, Mark

1992-01-01T23:59:59.000Z

325

Thermal Management of Solar Cells  

E-Print Network [OSTI]

D. Mills, "Cooling of photovoltaic cells under concentratedelectric performance of a photovoltaic cells by cooling andSolar Cell A photovoltaic cell is a semiconductor that

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

326

Fuel Cells at NASCAR  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cells at NASCAR Ned Stetson U.S. Department of Energy Fuel Cell Technologies Office Catherine Kummer - NASCAR Green Norm Bessette - Acumentrics Question and Answer * Please...

327

Automotive Fuel Cell Corporation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

with AFCC, a private joint venture company in Canada, formed by combining the automotive fuel cell business of Ballard Power Systems with the fuel cell stack development...

328

SFTEL: Flow Cell | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Flow Cell EMSL's Subsurface Flow and Transport Experimental Laboratory offers several meter-scale flow cells and columns for research in saturated and unsaturated porous media....

329

Microfluidic fuel cells.  

E-Print Network [OSTI]

??Microfluidic fuel cell architectures are presented in this thesis. This work represents the mechanical and microfluidic portion of a microfluidic biofuel cell project. While the (more)

Kjeang, Erik

2007-01-01T23:59:59.000Z

330

Fuel Cell Technologies Office: Fuel Cell Technical Publications  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Cell Technical Cell Technical Publications to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technical Publications on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technical Publications on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technical Publications on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technical Publications on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technical Publications on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technical Publications on AddThis.com... Publications Program Publications Technical Publications Hydrogen Fuel Cells Safety, Codes & Standards Market Analysis Educational Publications Newsletter Program Presentations Multimedia Conferences & Meetings

331

The Transmission of Avian Visceral Lymphomatosis by Contact  

Science Journals Connector (OSTI)

...attempts to infect cell lines derived from normal...for experimental transmission of mammary tumor...attempts to infect cell lines derived from normal...for experimental transmission of mammary tumor...cultures, the 3 cell lines, found to contain...intraperitoneal routes into BALB/c and...

B. R. Burmester and R. F. Gentry

1954-01-01T23:59:59.000Z

332

Sprouty-4 Inhibits Transformed Cell Growth, Migration and Invasion, and Epithelial-Mesenchymal Transition, and Is Regulated by Wnt7A through PPAR? in NonSmall Cell Lung Cancer  

Science Journals Connector (OSTI)

...West J, Franklin W, et al. Altered HOX and WNT7A expression in human lung cancer. Proc Natl Acad Sci U S A 2000;97:12776-81. 55 Kirikoshi H , Katoh M. Expression of WNT7A in human normal tissues and cancer, and regulation of WNT7A and WNT7B...

Meredith A. Tennis; Michelle M. Van Scoyk; Scott V. Freeman; Katherine M. Vandervest; Raphael A. Nemenoff; Robert A. Winn

2010-06-01T23:59:59.000Z

333

Final Report: Latent Expression of Genetic Damage in Human Lung Cells  

SciTech Connect (OSTI)

This project was aimed at furthering understanding of the latent effects of ionizing radiation. The underlying premise was that such latent (i.e., delayed) effects stemmed from radiation-induced genetic instability. As model system to investigate certain aspects of genomic instability, they proposed to look at chromosomal instability involving quasi-targeted radiation-induced breakpoints in the vicinity of the HPRT gene in EJ30 human epithelial cells. Using whole chromosome painting of the X chromosome, the authors were able to show that about 15% of randomly selected 6-thioguanine resistant (6TG{prime}) mutants involved translocations in the terminal portion of Xq. Subsequent analysis, using human genomic YAC probes confirmed that all the translocations were either within (or near Xq26.1), the cytogenetic location of HPRT, whereas none were found elsewhere involving the X chromosome.

Cornforth, Michael N.

1999-02-28T23:59:59.000Z

334

Epigenetic alterations mapped with human promoter tiling microarrays identify genes implicated in epithelial-mesenchymal transition in a breast cancer metastasis model.  

Science Journals Connector (OSTI)

...of gene expression might contribute directly to promotion of metastasis. Metastasis is a complex process involving sequential and interrelated steps. In this study, we have examined the impact of EWS/FLI expression on cell migration, cell...

David Rodenhiser; Joseph Andrews; Wendy Kennette; Bekim Sadikovic; Ariel Mendlowitz; Jenna Pilon; Alan Tuck; and Ann Chambers

2008-05-01T23:59:59.000Z

335

Fuel Cell Technologies Office: Reversible Fuel Cells Workshop  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Reversible Fuel Cells Reversible Fuel Cells Workshop to someone by E-mail Share Fuel Cell Technologies Office: Reversible Fuel Cells Workshop on Facebook Tweet about Fuel Cell Technologies Office: Reversible Fuel Cells Workshop on Twitter Bookmark Fuel Cell Technologies Office: Reversible Fuel Cells Workshop on Google Bookmark Fuel Cell Technologies Office: Reversible Fuel Cells Workshop on Delicious Rank Fuel Cell Technologies Office: Reversible Fuel Cells Workshop on Digg Find More places to share Fuel Cell Technologies Office: Reversible Fuel Cells Workshop on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Program Presentations Multimedia Conferences & Meetings Annual Merit Review Proceedings Workshop & Meeting Proceedings

336

Origins and consequences of radiation…induced centrosome aberrations  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Origins and consequences of radiation-induced centrosome aberrations Origins and consequences of radiation-induced centrosome aberrations Sangeetha Vijayakumar, Nisarg Shah, Ignacio Fernandez-Garcia, Mary Helen Barcellos-Hoff Department of Radiation Oncology, New York University School of Medicine, NY, NY. Centrosome aberrations are frequently observed in pre-neoplastic breast lesions and are known to drive chromosomal instability (Lingle et al., 2002). Previous studies from our lab have shown that human mammary epithelial cells exposed to low doses of radiation exhibit centrosome aberrations (CAs) in a dose dependent manner from 10-200 cGy (Maxwell et al., 2008). These data demonstrated that radiation-induced CAs actually precede and generate genomic instability and that TGFβ is a key mediator

337

Defining the Role of Histone Deacetylases in the Inhibition of Mammary Carcinogenesis by Dietary Energy Restriction (DER): Effects of Suberoylanilide Hydroxamic Acid (SAHA) and DER in a Rat Model  

Science Journals Connector (OSTI)

...dietary SAHA and/or dietary energy restriction on regulatory...dietary SAHA and/or dietary energy restriction on plasma analytes...road less traveled. In: New York, NY:McTiernan A, editor...Thompson HJ.Effect of energy restriction on cell cycle...

Zongjian Zhu; Weiqin Jiang; John N. McGinley; and Henry J. Thompson

2013-04-01T23:59:59.000Z

338

Electrocatalysts for Fuel Cells  

Science Journals Connector (OSTI)

...research-article Electrocatalysts for Fuel Cells G. J. K. Acres G. A. Hards The...physical composition of the catalysts used in fuel cells are determined by the type of cell...operating conditions. The six types of fuel cell presently in use or under development...

1996-01-01T23:59:59.000Z

339

FCT Fuel Cells: Basics  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Basics to someone by E-mail Basics to someone by E-mail Share FCT Fuel Cells: Basics on Facebook Tweet about FCT Fuel Cells: Basics on Twitter Bookmark FCT Fuel Cells: Basics on Google Bookmark FCT Fuel Cells: Basics on Delicious Rank FCT Fuel Cells: Basics on Digg Find More places to share FCT Fuel Cells: Basics on AddThis.com... Home Basics Current Technology DOE R&D Activities Quick Links Hydrogen Production Hydrogen Delivery Hydrogen Storage Technology Validation Manufacturing Codes & Standards Education Systems Analysis Contacts Basics Photo of a fuel cell stack A fuel cell uses the chemical energy of hydrogen to cleanly and efficiently produce electricity with water and heat as byproducts. (How much water?) Fuel cells are unique in terms of the variety of their potential applications; they can provide energy for systems as large as a utility

340

HISTORY | Fuel Cells  

Science Journals Connector (OSTI)

Together with the electric motor, dynamo, gas turbine, internal combustion engine, and the fused salt electrolysis of aluminum, the industrial revolution of the nineteenth century brought about the fuel cell the silent or cold combustion of fossil fuels by the electrochemical oxidation with atmospheric oxygen to water and carbon dioxide. Wilhelm Ostwald, in 1894, emphasized the high efficiency and the nonpolluting properties of the direct conversion of chemical energy into electricity in contrast to the then combination of steam engine and dynamo, which reached only about 10% efficiency. Direct coal fuel cells designed for the propulsion of ships, however, have not become a reality so far. Instead of fuel cells and batteries, internal combustion engines determined the nineteenth- and twentieth- century technological landscape. Against the background of the oil crisis and the long-term scarcity of natural gas, crude oil, and coal, new hopes have focused on fuel cell technology, which saw first early splendid applications during the space programs of the 1960s, in submarines since the 1980s, and in experimental zero-emission vehicles (ZEVs) since the 1990s. This article outlines (1) early insights about energy conversion: Grove's cell, direct conversion of coal and indirect fuel cells; (2) historical roots of alkaline fuel cells: the discovery of gas diffusion electrodes; low-pressure alkaline fuel cell conquer spacecrafts and submarines; (3) polymer electrolyte fuel cells: solid polymer technology, electric vehicles, direct methanol fuel-cell, stationary power systems and portable polymer electrolyte membrane fuel cell systems; (4) phosphoric acid fuel cell (PAFC): acid fuel cells, PAFC plants in Japan, gasoline fuel cells; and (5) high-temperature fuel cells: molten carbonate fuel cell and solid oxide fuel cell.

P. Kurzweil

2009-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
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341

Fuel Cell Technologies Overview  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

4/3/2012 4/3/2012 eere.energy.gov Fuel Cell Technologies Overview Flow Cell Workshop Washington, DC Dr. Sunita Satyapal & Dr. Dimitrios Papageorgopoulos U.S. Department of Energy Fuel Cell Technologies Program 3/7/2011 Flow Cells for Energy Storage Workshop Purpose To understand the applied research and development needs and the grand challenges for the use of flow cells as energy-storage devices. Objectives 1. Understand the needs for applied research from stakeholders. 2. Gather input for future development of roadmaps and technical targets for flow cells for various applications. 3. Identify grand challenges and prioritize R&D needs. Flow cells combine the unique advantages of batteries and fuel cells and can offer benefits for multiple energy storage applications.

342

FUEL CELLS RALLY  

Science Journals Connector (OSTI)

FUEL CELLS RALLY ... No, this car has composite tanks capable of storing 8 kg of hydrogen. ... It's General Motors' Sequel, a fuel-cell concept car unveiled earlier this month at the North American International Auto Show in Detroit. ...

ALEXANDER H. TULLO

2005-01-31T23:59:59.000Z

343

Semitransparent organic solar cells  

Science Journals Connector (OSTI)

The organic solar cell technology has attracted great interests due to ... low cost solution process capability. Bulk heterojunction organic solar cells offer a potentially much cheaper alternative way to harness...

Furong Zhu

2014-03-01T23:59:59.000Z

344

What determines cell size?  

E-Print Network [OSTI]

as: Marshall WF, et al. : What determines cell size? BMC7007/10/101 FORUM Open Access What determines cell size?biologists have been wondering what determines the size of

2012-01-01T23:59:59.000Z

345

fuel cells | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

fuel cells fuel cells Leads No leads are available at this time. The Molecular Bond: October 2014 The Molecular Bond newsletter banner October 2014 FROM THE DIRECTOR Read more...

346

NANOCOMPOSITE ENABLED SENSITIZED SOLAR CELL  

E-Print Network [OSTI]

in all thin-film solar cell technologies is that absorbancecells. These emerging solar cell technologies have undergonethe various solar cell technologies and their progress as

Phuyal, Dibya

2012-01-01T23:59:59.000Z

347

NANOCOMPOSITE ENABLED SENSITIZED SOLAR CELL  

E-Print Network [OSTI]

by Dye-Sensitized Photovoltaic cells. Inorganic Chemistry,by Dye-Sensitized Photovoltaic Cells. Inorganic ChemistryThe characteristics of a photovoltaic cell. Generally,

Phuyal, Dibya

2012-01-01T23:59:59.000Z

348

DOE Fuel Cell Technologies Office  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

DOE Fuel Cell Technologies Office Fuel Cell Seminar & Energy Exposition Columbus, Ohio Dr. Sunita Satyapal Director Fuel Cell Technologies Office Energy Efficiency and Renewable...

349

Fuel Cells | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cells Fuel Cells Fuel cells are an important enabling technology for the nation's energy portfolio and have the potential to revolutionize the way we power our nation,...

350

Webinar: Fuel Cell Buses  

Broader source: Energy.gov [DOE]

Video recording and text version of the webinar titled, Fuel Cell Buses, originally presented on September 12, 2013.

351

Electrochemistry Cell Model  

Broader source: Energy.gov [DOE]

2012 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

352

Photo-Electric Cells  

Science Journals Connector (OSTI)

... be measured, and its variation studied with variation of the incident light. Again, the photo-electric current may be amplified by valve circuits used outside the cell, or may ... to the infra-red, in which the active substance is oxidised thallium sulphide), barium photo-electric cells, sodium, and selenium cells.

ALLAN FERGUSON

1930-06-21T23:59:59.000Z

353

Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Market Transformation Market Transformation Printable Version Share this resource Send a link to Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies to someone by E-mail Share Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies on Facebook Tweet about Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies on Twitter Bookmark Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies on Google Bookmark Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies on Delicious Rank Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies on Digg Find More places to share Fuel Cell Technologies Office: Early Adoption of Fuel Cell Technologies on AddThis.com... Early Adoption of Fuel Cells Early Market Applications for Fuel Cells

354

California: TetraCell Silicon Solar Cell Improves Efficiency...  

Energy Savers [EERE]

California: TetraCell Silicon Solar Cell Improves Efficiency, Wins R&D 100 Award California: TetraCell Silicon Solar Cell Improves Efficiency, Wins R&D 100 Award August 16, 2013 -...

355

DOE Fuel Cell Technologies Office Record 14012: Fuel Cell System...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

2: Fuel Cell System Cost - 2013 DOE Fuel Cell Technologies Office Record 14012: Fuel Cell System Cost - 2013 This program record from the U.S. Department of Energy's Fuel Cell...

356

FUEL CELLS MOLTEN CARBONATE FUEL CELLS | Overview  

Science Journals Connector (OSTI)

The molten carbonate fuel cell (MCFC) emerged during the twentieth century as one of the key fuel cell types. It uses an electrolyte of alkali metal carbonates, operates typically at 650C, and is best suited to hydrocarbon fuels such as natural gas, coal gas, or biogas. The high operating temperature enables such fuels to be fed directly to the MCFC stacks, leading to conversion efficiencies greater than 50%. Molten carbonate fuel cell systems are ideally suited to applications that need continuous base load power. The first commercial systems, at the 300kW scale, are therefore being used in applications such as hospitals and hotels.

A.L. Dicks

2009-01-01T23:59:59.000Z

357

In vivo Effects of Vaccination with Six-Transmembrane Epithelial Antigen of the Prostate: A Candidate Antigen for Treating Prostate Cancer  

Science Journals Connector (OSTI)

...monitored twice at week with engineer calipers. Data of one of...T cells in their tumor environment. TIL were recovered from...adequate type 1 T-cell environment, events that are crucial...IL-12 levels in the tumor environment was associated with a favorable...

Maria de la Luz Garcia-Hernandez; Andrew Gray; Bolyn Hubby; and W. Martin Kast

2007-02-01T23:59:59.000Z

358

Fuel Cell Buses | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cell Buses Fuel Cell Buses Download presentation slides from the DOE Fuel Cell Technologies Office webinar "Fuel Cell Buses" held on September 12, 2013. Fuel Cell Buses...

359

Fuel Cells Fact Sheet | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Cells Fact Sheet Fuel Cells Fact Sheet Fact sheet produced by the Fuel Cell Technologies Office describing hydrogen fuel cell technology. Fuel Cells More Documents & Publications...

360

Cell Fusion and Tissue Regeneration  

Science Journals Connector (OSTI)

Cell fusion is a natural process implicated in normal ... bone marrow stem cells fuse with several cell types, under normal condition or after an injury ... in regenerative medicine and genetic repair. Cell fusion

Manuel lvarez-Dolado; Magdalena Martnez-Losa

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Abstract #174: Human Ductal Carcinoma In Situ contains malignant progenitor cells  

Science Journals Connector (OSTI)

...BRCA1 promoter hypermethylation does not hold promise for risk-stratifying...22 Jones PA, Baylin SB. The fundamental role of epigenetic events in...cancer-associated gene 1 promoter does not predict cytologic atypia...mathematical risk models and (b) does not predict mammary atypia in...

Virginia Espina; Lucia Pastore; Luana Adamo; Mariaelena Pierobon; Stacey Banks; Barbara Merritt; Jasbir Johal; Syed Zaman; Emanuel Petricoin; Kirsten Edmiston; and Lance Liotta

2009-05-01T23:59:59.000Z

362

Fuel Cells Team  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Judith Valerio at one of our 31 single-cell test stands Fuel Cell Team The FC team focus is R&D on polymer electrolyte membrane (PEM) fuel cells for commercial and military applications. Our program has had ongoing funding in the area of polymer electrolyte fuel cells since 1977 and has been responsible for enabling breakthroughs in the areas of thin film electrodes and air bleed for CO tolerance. For more information on the history of fuel cell research at Los Alamos, please click here. Fuel cells are an important enabling technology for the Hydrogen Economy and have the potential to revolutionize the way we power the nation and the world. The FC team is exploring the potential of fuel cells as energy-efficient, clean, and fuel-flexible alternatives that will

363

Webinar: Fuel Cell Mobile Lighting  

Broader source: Energy.gov [DOE]

Video recording of the Fuel Cell Technologies Office webinar, Fuel Cell Mobile Lighting, originally presented on November 13, 2012.

364

Effets de la prolactine sur la scrtion des lipides du lait dans les cellules pithliales mammaires  

E-Print Network [OSTI]

-en-Josas, France. Summary. Effect of prolactin on milk lipid secretion in lactating rabbit mammary gland epithelial secretion. These results show that the radioactivity of neosynthesized lipid constituents was modified during the secretion of milk lipids. Prolactin, which increased the total labelled lipids secreted, had

Boyer, Edmond

365

Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Joint Fuel Cell Bus Joint Fuel Cell Bus Workshop to someone by E-mail Share Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop on Facebook Tweet about Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop on Twitter Bookmark Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop on Google Bookmark Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop on Delicious Rank Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop on Digg Find More places to share Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Program Presentations Multimedia Conferences & Meetings Annual Merit Review Proceedings Workshop & Meeting Proceedings Webinars

366

Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Flow Cells for Energy Flow Cells for Energy Storage Workshop to someone by E-mail Share Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop on Facebook Tweet about Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop on Twitter Bookmark Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop on Google Bookmark Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop on Delicious Rank Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop on Digg Find More places to share Fuel Cell Technologies Office: Flow Cells for Energy Storage Workshop on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Program Presentations Multimedia Conferences & Meetings Annual Merit Review Proceedings

367

Fuel Cell Technologies Office: Early Market Applications for Fuel Cell  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Market Transformation Market Transformation Printable Version Share this resource Send a link to Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies to someone by E-mail Share Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies on Facebook Tweet about Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies on Twitter Bookmark Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies on Google Bookmark Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies on Delicious Rank Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies on Digg Find More places to share Fuel Cell Technologies Office: Early Market Applications for Fuel Cell Technologies on AddThis.com...

368

Atmospheric pressure gas plasma-induced colorectal cancer cell death is mediated by Nox2ASK1 apoptosis pathways and oxidative stress is mitigated by SrxNrf2 anti-oxidant system  

Science Journals Connector (OSTI)

Abstract Atmospheric pressure gas plasma (AGP) generates reactive oxygen species (ROS) that induce apoptosis in cultured cancer cells. The majority of cancer cells develop a ROS-scavenging anti-oxidant system regulated by Nrf2, which confers resistance to ROS-mediated cancer cell death. Generation of ROS is involved in the AGP-induced cancer cell death of several colorectal cancer cells (Caco2, HCT116 and SW480) by activation of ASK1-mediated apoptosis signaling pathway without affecting control cells (human colonic sub-epithelial myofibroblasts; CO18, human fetal lung fibroblast; MRC5 and fetal human colon; FHC). However, the identity of an oxidase participating in AGP-induced cancer cell death is unknown. Here, we report that AGP up-regulates the expression of Nox2 (NADPH oxidase) to produce ROS. RNA interference designed to target Nox2 effectively inhibits the AGP-induced ROS production and cancer cell death. In some cases both colorectal cancer HT29 and control cells showed resistance to AGP treatment. Compared to AGP-sensitive Caco2 cells, HT29 cells show a higher basal level of the anti-oxidant system transcriptional regulator Nrf2 and its target protein sulfiredoxin (Srx) which are involved in cellular redox homeostasis. Silencing of both Nrf2 and Srx sensitized HT29 cells, leads to ROS overproduction and decreased cell viability. This indicates that in HT29 cells, Nrf2/Srx axis is a protective factor against AGP-induced oxidative stress. The inhibition of Nrf2/Srx signaling should be considered as a central target in drug-resistant colorectal cancer treatments.

Musarat Ishaq; Margaret D.M. Evans; Kostya (Ken) Ostrikov

2014-01-01T23:59:59.000Z

369

Fuel Cells publications  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Materials Science » Materials Science » Fuel Cells » Fuel Cells Publications Fuel Cells publications Research into alternative forms of energy, especially energy security, is one of the major national security imperatives of this century. Get Expertise Melissa Fox Applied Energy Email Catherine Padro Sensors & Electorchemical Devices Email Fernando Garzon Sensors & Electorchemical Devices Email Piotr Zelenay Sensors & Electorchemical Devices Email Rod Borup Sensors & Electorchemical Devices Email Karen E. Kippen Chemistry Communications Email Like a battery, a fuel cell consists of two electrodes separated by an electrolyte-in polymer electrolyte fuel cells, the separator is made of a thin polymeric membrane. Unlike a battery, a fuel cell does not need recharging-it continues to produce electricity as long as fuel flows

370

Fuel Cells Overview  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Hydrogen Storage DELIVERY FUEL CELLS STORAGE PRODUCTION TECHNOLOGY VALIDATION CODES & STANDARDS SYSTEMS INTEGRATION / ANALYSES SAFETY EDUCATION RESEARCH & DEVELOPMENT Economy Pat Davis 2 Fuel Cells Technical Goals & Objectives Goal : Develop and demonstrate fuel cell power system technologies for transportation, stationary, and portable applications. 3 Fuel Cells Technical Goals & Objectives Objectives * Develop a 60% efficient, durable, direct hydrogen fuel cell power system for transportation at a cost of $45/kW (including hydrogen storage) by 2010. * Develop a 45% efficient reformer-based fuel cell power system for transportation operating on clean hydrocarbon or alcohol based fuel that meets emissions standards, a start-up time of 30 seconds, and a projected manufactured cost of $45/kW by

371

Distributed Energy Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Energy Fuel Cells Energy Fuel Cells DOE Hydrogen DOE Hydrogen and and Fuel Cells Fuel Cells Coordination Meeting Fuel Cell Coordination Meeting June 2-3, 2003 Electricity Users Kathi Epping Kathi Epping Objectives & Barriers Distributed Energy OBJECTIVES * Develop a distributed generation PEM fuel cell system operating on natural gas or propane that achieves 40% electrical efficiency and 40,000 hours durability at $400-750/kW by 2010. BARRIERS * Durability * Heat Utilization * Power Electronics * Start-Up Time Targets and Status Integrated Stationary PEMFC Power Systems Operating on Natural Gas or Propane Containing 6 ppm Sulfur 40,000 30,000 15,000 Hours Durability 750 1,250 2,500 $/kWe Cost 40 32 30 % Electrical Efficiency Large (50-250 kW) Systems 40,000 30,000 >6,000 Hours Durability 1,000 1,500 3,000

372

How Fuel Cells Work  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

How Fuel Cells Work How Fuel Cells Work Diagram: How a PEM fuel cell works. 1. Hydrogen fuel is channeled through field flow plates to the anode on one side of the fuel cell, while oxygen from the air is channeled to the cathode on the other side of the cell. 2. At the anode, a platinum catalyst causes the hydrogen to split into positive hydrogen ions (protons) and negatively charged electrons. 3. The Polymer Electrolyte Membrane (PEM) allows only the positively charged ions to pass through it to the cathode. The negatively charged electrons must travel along an external circuit to the cathode, creating an electrical current. 4. At the cathode, the electrons and positively charged hydrogen ions combine with oxygen to form water, which flows out of the cell.

373

Fuel cell generating plant  

SciTech Connect (OSTI)

This paper discusses a fuel cell generating plant. It comprises a compressed fuel supply; a fuel cell system including fuel conditioning apparatus and fuel cells; a main fuel conduit for conveying fuel from the fuel supply to the fuel cell system; a turbo compressor having a turbine receiving exhaust products from the fuel cell system and a compressor for compressing air; a main air conduit for conveying air from the compressor to the fuel cell system; an auxiliary burner having a primary burner and a pilot; an auxiliary air conduit for conveying air from the compressed fuel supply to the auxiliary burner; an auxiliary exhaust conduit for conveying exhaust products from the auxiliary burner to the turbine; a check valve located between the fuel supply and the pilot; and a gas accumulator in the auxiliary fuel conduit located between the check valve and the pilot.

Sanderson, R.A.

1990-11-27T23:59:59.000Z

374

Concentrator silicon cell research  

SciTech Connect (OSTI)

This project continued the developments of high-efficiency silicon concentrator solar cells with the goal of achieving a cell efficiency in the 26 to 27 percent range at a concentration level of 150 suns of greater. The target efficiency was achieved with the new PERL (passivated emitter, rear locally diffused) cell structure, but only at low concentration levels around 20 suns. The PERL structure combines oxide passivation of both top and rear surfaces of the cells with small area contact to heavily doped regions on the top and rear surfaces. Efficiency in the 22 to 23 percent range was also demonstrated for large-area concentrator cells fabricated with the buried contact solar cell processing sequence, either when combined with prismatic covers or with other innovative approaches to reduce top contact shadowing. 19 refs.

Green, M.A.; Wenham, S.R.; Zhang, F.; Zhao, J.; Wang, A. [New South Wales Univ., Kensington (Australia). Solar Photovoltaic Lab.

1992-04-01T23:59:59.000Z

375

FUEL CELLS SOLID OXIDE FUEL CELLS | Systems  

Science Journals Connector (OSTI)

In this article, some basic arrangements of solid oxide fuel cell (SOFC) systems are described, starting with atmospheric systems using a catalytic burner or a thermal burner and anode gas recycling. For illustrating the potential electrical efficiency of SOFC systems, their combination with a gas turbine and also with a steam turbine (ST) are described. To be able to evaluate the potential of the different systems, first the essential efficiencies relevant to fuel cell systems are defined and then the basics of calculating energy balance are illustrated. Equations are given to describe, for example, the effect of fuel recycling on system fuel utilization and of internal reforming on the necessary air flow for cooling the stack. It is obvious that electrical efficiency depends strongly on cell voltage and fuel utilization. In the case of cells that operate with a high fuel utilization at cell voltages of 800mV, a net electrical efficiency above 55% can be achieved. The combination in a pressurized system with a gas turbine enables efficiencies of up to 70% and combining this system with an additional ST allows efficiencies of up to 75%. However, an investigation into the size of these \\{STs\\} shows that such combined systems make sense only above a gas input of 10MW.

L. Blum; E. Riensche

2009-01-01T23:59:59.000Z

376

Cascade Organic Solar Cells  

Science Journals Connector (OSTI)

Cascade Organic Solar Cells ... Multiple factors control the efficiency of organic solar cells, making it difficult to use single donor or acceptor materials to balance the, often opposing, material properties required to optimize device performance. ... We demonstrate planar organic solar cells consisting of a series of complementary donor materials with cascading exciton energies, incorporated in the following structure: glass/indium-tin-oxide/donor cascade/C60/bathocuproine/Al. ...

Cody W. Schlenker; Vincent S. Barlier; Stephanie W. Chin; Matthew T. Whited; R. Eric McAnally; Stephen R. Forrest; Mark E. Thompson

2011-09-02T23:59:59.000Z

377

Heterojunction solar cell  

DOE Patents [OSTI]

A high-efficiency single heterojunction solar cell is described wherein a thin emitter layer (preferably Ga[sub 0.52]In[sub 0.48]P) forms a heterojunction with a GaAs absorber layer. The conversion efficiency of the solar cell is at least 25.7%. The solar cell preferably includes a passivating layer between the substrate and the absorber layer. An anti-reflection coating is preferably disposed over the emitter layer. 1 fig.

Olson, J.M.

1994-08-30T23:59:59.000Z

378

Molten salt lithium cells  

DOE Patents [OSTI]

Lithium-based cells are promising for applications such as electric vehicles and load-leveling for power plants since lithium is very electropositive and light weight. One type of lithium-based cell utilizes a molten salt electrolyte and is operated in the temperature range of about 400 to 500/sup 0/C. Such high temperature operation accelerates corrosion problems and a substantial amount of energy is lost through heat transfer. The present invention provides an electrochemical cell which may be operated at temperatures between about 100 to 170/sup 0/C. The cell is comprised of an electrolyte, which preferably includes lithium nitrate, and a lithium or lithium alloy electrode.

Raistrick, I.D.; Poris, J.; Huggins, R.A.

1980-07-18T23:59:59.000Z

379

Molten salt lithium cells  

DOE Patents [OSTI]

Lithium-based cells are promising for applications such as electric vehicles and load-leveling for power plants since lithium is very electropositive and light weight. One type of lithium-based cell utilizes a molten salt electrolyte and is operated in the temperature range of about 400.degree.-500.degree. C. Such high temperature operation accelerates corrosion problems and a substantial amount of energy is lost through heat transfer. The present invention provides an electrochemical cell (10) which may be operated at temperatures between about 100.degree.-170.degree. C. Cell (10) comprises an electrolyte (16), which preferably includes lithium nitrate, and a lithium or lithium alloy electrode (12).

Raistrick, Ian D. (Menlo Park, CA); Poris, Jaime (Portola Valley, CA); Huggins, Robert A. (Stanford, CA)

1982-02-09T23:59:59.000Z

380

Microcomposite Fuel Cell Membranes  

Broader source: Energy.gov [DOE]

Summary of microcomposite fuel cell membrane work presented to the High Temperature Membrane Working Group Meeting, Orlando FL, October 17, 2003

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Fuel Cell Financing Options  

Broader source: Energy.gov [DOE]

Presented at the Clean Energy States Alliance and U.S. Department of Energy Webinar: Financing Fuel Cell Installations, August 30, 2011.

382

A chlorophyll solar cell  

Science Journals Connector (OSTI)

A simple chlorophyll solar cell has been designed built and tested. A voltage output versus time curve has also been obtained. (AIP)

J. Christopher Ludlow

1982-01-01T23:59:59.000Z

383

Fuel Cell Case Study  

Broader source: Energy.gov [DOE]

Presented at the Clean Energy States Alliance and U.S. Department of Energy Webinar: Fuel Cells for Supermarkets, April 4, 2011.

384

Modelling microscale fuel cells.  

E-Print Network [OSTI]

??The focus of this work is to investigate transport phenomena in recently developed microscale fuel cell designs using computational fluid dynamics (CFD). Two microscale fuel (more)

Bazylak, Aimy Ming Jii

2009-01-01T23:59:59.000Z

385

Mammalian Cell Culture | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mammalian Cell Culture At EMSL, researchers use the single-molecule fluorescencepatch clamp microscope to combine high-sensitivity fluorescence imaging simultaneously with...

386

Fuel Cell Technologies Overview  

Broader source: Energy.gov (indexed) [DOE]

Cells Key Benefits Very High Efficiency Reduced CO 2 Emissions Reduced Oil Use Reduced Air Pollution Fuel Flexibility * 40 - 60% (electrical) * > 70% (electrical, hybrid fuel...

387

Hydrogen Fuel Cells  

Fuel Cell Technologies Publication and Product Library (EERE)

The fuel cell an energy conversion device that can efficiently capture and use the power of hydrogen is the key to making it happen.

388

Quantum Junction Solar Cells  

Science Journals Connector (OSTI)

Quantum Junction Solar Cells ... Department of Electrical and Computer Engineering, University of Toronto, 10 Kings College Road, Toronto, Ontario, M5S 3G4, Canada ...

Jiang Tang; Huan Liu; David Zhitomirsky; Sjoerd Hoogland; Xihua Wang; Melissa Furukawa; Larissa Levina; Edward H. Sargent

2012-08-10T23:59:59.000Z

389

Fuel Cell Demonstration Program  

SciTech Connect (OSTI)

In an effort to promote clean energy projects and aid in the commercialization of new fuel cell technologies the Long Island Power Authority (LIPA) initiated a Fuel Cell Demonstration Program in 1999 with six month deployments of Proton Exchange Membrane (PEM) non-commercial Beta model systems at partnering sites throughout Long Island. These projects facilitated significant developments in the technology, providing operating experience that allowed the manufacturer to produce fuel cells that were half the size of the Beta units and suitable for outdoor installations. In 2001, LIPA embarked on a large-scale effort to identify and develop measures that could improve the reliability and performance of future fuel cell technologies for electric utility applications and the concept to establish a fuel cell farm (Farm) of 75 units was developed. By the end of October of 2001, 75 Lorax 2.0 fuel cells had been installed at the West Babylon substation on Long Island, making it the first fuel cell demonstration of its kind and size anywhere in the world at the time. Designed to help LIPA study the feasibility of using fuel cells to operate in parallel with LIPA's electric grid system, the Farm operated 120 fuel cells over its lifetime of over 3 years including 3 generations of Plug Power fuel cells (Lorax 2.0, Lorax 3.0, Lorax 4.5). Of these 120 fuel cells, 20 Lorax 3.0 units operated under this Award from June 2002 to September 2004. In parallel with the operation of the Farm, LIPA recruited government and commercial/industrial customers to demonstrate fuel cells as on-site distributed generation. From December 2002 to February 2005, 17 fuel cells were tested and monitored at various customer sites throughout Long Island. The 37 fuel cells operated under this Award produced a total of 712,635 kWh. As fuel cell technology became more mature, performance improvements included a 1% increase in system efficiency. Including equipment, design, fuel, maintenance, installation, and decommissioning the total project budget was approximately $3.7 million.

Gerald Brun

2006-09-15T23:59:59.000Z

390

REVIEW Open Access Cell therapy using tolerogenic dendritic cells in  

E-Print Network [OSTI]

with cell therapy using regulatory cells. In our laboratory, as part of a European project, we plan to testREVIEW Open Access Cell therapy using tolerogenic dendritic cells in transplantation Aurélie Moreau the safety of tolerogenic dendritic cell (TolDC) therapy in kidney transplant patients. In this mini-review

Paris-Sud XI, Université de

391

cell trait? Know your sickle cell trait status.  

E-Print Network [OSTI]

What is sickle cell trait? Know your sickle cell trait status. Engage in a slow and gradual experiencing unusual physical distress. People at high risk for having sickle cell trait are those whose countries. sickle cell trait is not a disease. Sickle cell trait is the inheritance of one gene for sickle

Devoto, Stephen H.

392

MiR-145 regulates PAK4 via the MAPK pathway and exhibits an antitumor effect in human colon cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer MiR-145 targets a putative binding site in the 3 Prime UTR of PAK4. Black-Right-Pointing-Pointer MiR-145 played an important role in inhibiting cell growth by directly targeting PAK4. Black-Right-Pointing-Pointer MiR-145 may function as tumor suppressors. -- Abstract: MicroRNAs (miRNAs) are regulators of numerous cellular events; accumulating evidence indicates that miRNAs play a key role in a wide range of biological functions, such as cellular proliferation, differentiation, and apoptosis in cancer. Down-regulated expression of miR-145 has been reported in colon cancer tissues and cell lines. The molecular mechanisms underlying miR-145 and the regulation of colon carcinogenesis remain unclear. In this study, we investigated the levels of miR-145 in human colon cancer cells using qRT-PCR and found markedly decreased levels compared to normal epithelial cells. We identified PAK4 as a novel target of miR-145 using informatics screening. Additionally, we demonstrated that miR-145 targets a putative binding site in the 3 Prime UTR of PAK4 and that its abundance is inversely associated with miR-145 expression in colon cancer cells; we confirmed this relationship using the luciferase reporter assay. Furthermore, restoration of miR-145 by mimics in SW620 cells significantly attenuated cell growth in vitro, in accordance with the inhibitory effects induced by siRNA mediated knockdown of PAK4. Taken together, these findings demonstrate that miR-145 downregulates P-ERK expression by targeting PAK4 and leads to inhibition of tumor growth.

Wang, Zhigang [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People's Hospital, Shanghai (China)] [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People's Hospital, Shanghai (China); Zhang, Xiaoping [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine (China)] [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine (China); Yang, Zhili; Du, Hangxiang; Wu, Zhenqian; Gong, Jianfeng; Yan, Jun [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People's Hospital, Shanghai (China)] [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People's Hospital, Shanghai (China); Zheng, Qi, E-mail: zhengqi1957@yahoo.com.cn [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People's Hospital, Shanghai (China)] [Department of General Surgery, Shanghai Jiaotong University Affiliated 6th People's Hospital, Shanghai (China)

2012-10-26T23:59:59.000Z

393

Fuel cell generator  

DOE Patents [OSTI]

High temperature solid oxide electrolyte fuel cell generators which allow controlled leakage among plural chambers in a sealed housing. Depleted oxidant and fuel are directly reacted in one chamber to combust remaining fuel and preheat incoming reactants. The cells are preferably electrically arranged in a series-parallel configuration.

Isenberg, Arnold O. (Forest Hills, PA)

1983-01-01T23:59:59.000Z

394

NETL: Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cells Fuel Cells Coal and Power Systems Fuel Cells SECA Logo Welcome to NETL's Fuel Cells Webpage. In partnership with private industry, educational institutions and national laboratories, we are leading the research, development, and demonstration of high efficiency, fuel flexible solid oxide fuel cells (SOFCs) and coal-based SOFC power generation systems for stationary market large central power plants under the Solid State Energy Conversion Alliance (SECA). The SECA cost reduction goal is to have SOFC systems capable of being manufactured at $400 per kilowatt by 2010. Concurrently, the scale-up, aggregation, and integration of the technology will progress in parallel leading to prototype validation of megawatt (MW)-class fuel flexible products by 2012 and 2015. The SECA coal-based systems goal is the development of large

395

Fuel Cell Development Status  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Development Status Michael Short Systems Engineering Manager United Technologies Corporation Research Center Hamilton Sundstrand UTC Power UTC Fire & Security Fortune 50 corporation $52.9B in annual sales in 2009 ~60% of Sales are in building technologies Transportation Stationary Fuel Cells Space & Defense * Fuel cell technology leader since 1958 * ~ 550 employees * 768+ Active U.S. patents, more than 300 additional U.S. patents pending * Global leader in efficient, reliable, and sustainable fuel cell solutions UTC Power About Us PureCell ® Model 400 Solution Process Overview Power Conditioner Converts DC power to high-quality AC power 3 Fuel Cell Stack Generates DC power from hydrogen and air 2 Fuel Processor Converts natural gas fuel to hydrogen

396

Fuel Cell 101  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cell 101 Fuel Cell 101 Don Hoffman Don Hoffman Ship Systems & Engineering Research Division March 2011 Distribution Statement A: Approved for public release; distribution is unlimited. Fuel Cell Operation * A Fuel Cell is an electrochemical power source * It supplies electricity by combining hydrogen and oxygen electrochemically without combustion. * It is configured like a battery with anode and cathode. * Unlike a battery, it does not run down or require recharging and will produce electricity and will produce electricity, heat and water as long as fuel is supplied. 2H + + 2e - O 2 + 2H + + 2e - 2H 2 O H 2 Distribution Statement A: Approved for public release; distribution is unlimited. 2 FUEL FUEL CONTROLS Fuel Cell System HEAT & WATER CLEAN CLEAN EXHAUST EXHAUST

397

NREL: Learning - Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cells Fuel Cells Fuel cells and their ability to cleanly produce electricity from hydrogen and oxygen are what make hydrogen attractive as a "fuel" for transportation use particularly, but also as a general energy carrier for homes and other uses, and for storing and transporting otherwise intermittent renewable energy. Fuel cells function somewhat like a battery-with external fuel being supplied rather than stored electricity-to generate power by chemical reaction rather than combustion. Hydrogen fuel cells, for instance, feed hydrogen gas into an electrode that contains a catalyst, such as platinum, which helps to break up the hydrogen molecules into positively charged hydrogen ions and negatively charged electrons. The electrons flow from the electrode to a terminal that

398

Cell manipulation in microfluidics  

Science Journals Connector (OSTI)

Recent advances in the lab-on-a-chip field in association with nano/microfluidics have been made for new applications and functionalities to the fields of molecular biology, genetic analysis and proteomics, enabling the expansion of the cell biology field. Specifically, microfluidics has provided promising tools for enhancing cell biological research, since it has the ability to precisely control the cellular environment, to easily mimic heterogeneous cellular environment by multiplexing, and to analyze sub-cellular information by high-contents screening assays at the single-cell level. Various cell manipulation techniques in microfluidics have been developed in accordance with specific objectives and applications. In this review, we examine the latest achievements of cell manipulation techniques in microfluidics by categorizing externally applied forces for manipulation: (i) optical, (ii) magnetic, (iii) electrical, (iv) mechanical and (v) other manipulations. We furthermore focus on history where the manipulation techniques originate and also discuss future perspectives with key examples where available.

Hoyoung Yun; Kisoo Kim; Won Gu Lee

2013-01-01T23:59:59.000Z

399

FORENSIC TECHNIQUES FOR CELL PHONES  

E-Print Network [OSTI]

June 2007 FORENSIC TECHNIQUES FOR CELL PHONES FORENSIC TECHNIQUES FOR CELL PHONES Shirley Radack cell phones are widely used for both personal and professional applications, the technology of cell forensics usually do not cover cell phones, especially those with advanced capabilities. The digital

400

Solid oxide fuel cell generator  

DOE Patents [OSTI]

A solid oxide fuel cell generator has a plenum containing at least two rows of spaced apart, annular, axially elongated fuel cells. An electrical conductor extending between adjacent rows of fuel cells connects the fuel cells of one row in parallel with each other and in series with the fuel cells of the adjacent row. 5 figures.

Di Croce, A.M.; Draper, R.

1993-11-02T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2 to someone by E-mail 2 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Archives Subscribe Program Presentations

402

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

August 2013 to someone by E-mail August 2013 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2013 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2013 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2013 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2013 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2013 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2013 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter

403

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

October 2012 to someone by E-mail October 2012 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: October 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: October 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: October 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: October 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: October 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: October 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications

404

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

April 2012 to someone by E-mail April 2012 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: April 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: April 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: April 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: April 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: April 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: April 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Archives

405

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

3 to someone by E-mail 3 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2013 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2013 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2013 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2013 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2013 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: May 2013 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Archives Subscribe Program Presentations

406

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2 to someone by E-mail 2 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: June 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: June 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: June 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: June 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: June 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: June 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Archives Subscribe

407

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

September/October 2013 to someone by E-mail September/October 2013 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September/October 2013 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September/October 2013 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September/October 2013 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September/October 2013 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September/October 2013 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September/October 2013 on AddThis.com... Publications

408

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

August 2012 to someone by E-mail August 2012 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: August 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter

409

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2 to someone by E-mail 2 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: March 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: March 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: March 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: March 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: March 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: March 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Archives Subscribe

410

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

3 to someone by E-mail 3 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: February 2013 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: February 2013 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: February 2013 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: February 2013 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: February 2013 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: February 2013 on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter

411

Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter:  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

September 2012 to someone by E-mail September 2012 to someone by E-mail Share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September 2012 on Facebook Tweet about Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September 2012 on Twitter Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September 2012 on Google Bookmark Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September 2012 on Delicious Rank Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September 2012 on Digg Find More places to share Fuel Cell Technologies Office: Fuel Cell Technologies Office Newsletter: September 2012 on AddThis.com... Publications Program Publications Technical Publications Educational Publications

412

A COMPARISON OF DNA DAMAGE PROBES IN TWO HMEC LINES WITH X-IRRADIATION  

SciTech Connect (OSTI)

In this study, we investigated ?H2AXser139 and 53BP1ser25, DNA damage pathway markers, to observe responses to radiation insult. Two Human Mammary Epithelial Cell (HMEC) lines were utilized to research the role of immortalization in DNA damage marker expression, HMEC HMT-3522 (S1) with an infi nite lifespan, and a subtype of HMEC 184 (184V) with a fi nite lifespan. Cells were irradiated with 50cGy X-rays, fi xed with 4% paraformaldehyde after 1 hour repair at 37C, and processed through immunofl uorescence. Cells were visualized with a fl uorescent microscope and images were digitally captured using Image-Pro Plus software. The 184V irradiated cells exhibited a more positive punctate response within the nucleus for both DNA damage markers compared to the S1 irradiated cells. The dose and time course will be expanded in future studies to augment the preliminary data from this research. It is important to understand whether the process of transformation to immortalization compromises the DNA damage sensor and repair process proteins of HMECs in order to understand what is normal and to evaluate the usefulness of cell lines as experimental models.

Wisnewski, C.L.; Bjornstad, K.A.; Rosen, C.J.; Chang, P.Y.; Blakely, E.A.

2007-01-01T23:59:59.000Z

413

A comparison of DNA damage probes in two HMEC lines withX-irradiation  

SciTech Connect (OSTI)

In this study, we investigated {gamma}H2AX{sup ser139} and 53BP1{sup ser25}, DNA damage pathway markers, to observe responses to radiation insult. Two Human Mammary Epithelial Cell (HMEC) lines were utilized to research the role of immortalization in DNA damage marker expression, HMEC HMT-3522 (S1) with an infinite lifespan, and a subtype of HMEC 184 (184V) with a finite lifespan. Cells were irradiated with 50 cGy X-rays, fixed with 4% paraformaldehyde after 1 hour repair at 37 C, and processed through immunofluorescence. Cells were visualized with a fluorescent microscope and images were digitally captured using Image-Pro Plus software. The 184V irradiated cells exhibited a more positive punctate response within the nucleus for both DNA damage markers compared to the S1 irradiated cells. We will expand the dose and time course in future studies to augment the preliminary data from this research. It is important to understand whether the process of transformation to immortalization compromises the DNA damage sensor and repair process proteins of HMECs in order to understand what is 'normal' and to evaluate the usefulness of cell lines as experimental models.

Wisnewski, Christy L.; Bjornstad, Kathleen A.; Rosen, ChristoperJ.; Chang, Polly Y.; Blakely, Eleanor A.

2007-01-19T23:59:59.000Z

414

www.cell-research.com | Cell Research EMT or apoptosis: a decision for TGF-  

E-Print Network [OSTI]

and epithelial-to-mesenchymal transition (EMT) are very fundamental physiological processes. They are both independent and interrelated events in nor- mal development and in maintaining body homeostasis. In recent and mesenchymal genes. Dysregulation of apoptosis and EMT are linked with various pathological processes

Cai, Long

415

Fuel Cell Animation- Fuel Cell Stack (Text Version)  

Broader source: Energy.gov [DOE]

This text version of the fuel cell animation demonstrates how a fuel cell uses hydrogen to produce electricity, with only water and heat as byproducts.

416

NREL: Hydrogen and Fuel Cells Research - Fuel Cell Electric Vehicle...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cell Electric Vehicle Evaluations NREL's technology validation team analyzes hydrogen fuel cell electric vehicles (FCEVs) operating in a real-world setting to identify the...

417

Fuel Cell Animation- Fuel Cell Components (Text Version)  

Broader source: Energy.gov [DOE]

This text version of the fuel cell animation demonstrates how a fuel cell uses hydrogen to produce electricity, with only water and heat as byproducts.

418

DOE Fuel Cell Technologies Office Record 14012: Fuel Cell System...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cell Technologies Office Record Record : 14012 Date: June 12, 2014 Title: Fuel Cell System Cost - 2013 Update to: Record 12020 Originator: Jacob Spendelow and Jason...

419

Red blood cell malformations Cell shapes Modeling and simulation of red blood cell light scattering  

E-Print Network [OSTI]

that have a "sickle" appearance (see figure to left). This malformed geometry prevents cells from traveling Red blood cell malformations Cell shapes Modeling and simulation of red blood cell light to various diseases and acute conditions, the shape and composition of erythrocytes (red blood cells

California at Berkeley, University of

420

Corrosion Test Cell For Bipolar Plates  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Corrosion Test Cell For Bipolar Plates Corrosion Test Cell For Bipolar Plates A corrosion test cell for evaluating corrosion resistance in fuel cell bipolar plates is described....

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

Energy 101: Fuel Cells | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Fuel Cells Energy 101: Fuel Cells Addthis Description Learn everything you need to know about fuel cells. Topic Hydrogen & Fuel Cells...

422

Types of Fuel Cells | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cells Current Technology Types of Fuel Cells Types of Fuel Cells Fuel cells are classified primarily by the kind of electrolyte they employ. This classification...

423

Fuel Cell Animation | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cell Animation Fuel Cell Animation This fuel cell animation demonstrates how a fuel cell uses hydrogen to produce electricity, with only water and heat as byproducts. Hydrogen...

424

Untangling the Fiber Yarn: Butyrate Feeds Warburg to Suppress Colorectal Cancer  

Science Journals Connector (OSTI)

...Butyrate represents the primary source of energy in normal colonic epithelial cells (6...colonic epithelial cells as a primary source of energy. However, cancer cells use glucose to obtain energy and feed anaplerotic reactions (Warburg...

Carlos Sebastin and Raul Mostoslavsky

2014-12-01T23:59:59.000Z

425

Fuel Cell Technologies Office: Publications  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cell Technologies Office HOME ABOUT PROGRAM AREAS INFORMATION RESOURCES FINANCIAL OPPORTUNITIES TECHNOLOGIES MARKET TRANSFORMATION NEWS EVENTS EERE Fuel Cell Technologies...

426

Module 5: Fuel Cell Systems  

Broader source: Energy.gov [DOE]

This course covers the systems required to operate a fuel cell engine, the components and functionality of each fuel cell system

427

Radiative cooling of solar cells  

Science Journals Connector (OSTI)

Standard solar cells heat up under sunlight. The resulting increased temperature of the solar cell has adverse consequences on both its efficiency and its reliability. We introduce a...

Zhu, Linxiao; Raman, Aaswath; Wang, Ken Xingze; Anoma, Marc Abou; Fan, Shanhui

2014-01-01T23:59:59.000Z

428

Slow elimination of phosphorylated histone {gamma}-H2AX from DNA of terminally differentiated mouse heart cells in situ  

SciTech Connect (OSTI)

Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around double-strand DNA breaks (DSBs) and this modification (called {gamma}-H2AX) may serve as a useful marker of genome damage and repair in terminally differentiated cells. Here using immunohistochemistry we studied kinetics of {gamma}-H2AX formation and elimination in the X-irradiated mouse heart and renal epithelial tissues in situ. Unirradiated tissues have 3-5% {gamma}-H2AX-positive cells and in tissues fixed 1 h after X-irradiation {gamma}-H2AX-positive nuclei are induced in a dose-dependent manner approaching 20-30% after 3 Gy of IR. Analysis of mouse tissues at different times after 3 Gy of IR showed that maximal induction of {gamma}-H2AX in heart is observed 20 min after IR and then is decreased slowly with about half remaining 23 h later. In renal epithelium maximum of the {gamma}-H2AX-positive cells is observed 40 min after IR and then decreases to control values in 23 h. This indicates that there are significant variations between non-proliferating mammalian tissues in the initial H2AX phosphorylation rate as well as in the rate of {gamma}-H2AX elimination after X-irradiation, which should be taken into account in the analysis of radiation responses.

Gavrilov, Boris [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Vezhenkova, Irina [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Firsanov, Denis [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Solovjeva, Liudmila [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Svetlova, Maria [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Mikhailov, Vyacheslav [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Tomilin, Nikolai [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation)]. E-mail: nvtom@hotmail.com

2006-09-08T23:59:59.000Z

429

Hypermethylation of let-7a-3 in Epithelial Ovarian Cancer Is Associated with Low Insulin-like Growth Factor-II Expression and Favorable Prognosis  

Science Journals Connector (OSTI)

...CpG sites in the let-7a-3 gene, Brueckner et al. (8) found 90 of the CpG sites...on the same DNA region as described by Brueckner et al., we developed a qMSP. Using...comparable with the experimental results of Brueckner et al. who found that cells treated...

Lingeng Lu; Dionyssios Katsaros; Irene A. Rigault de la Longrais; Olga Sochirca; Herbert Yu

2007-11-01T23:59:59.000Z

430

Abstract 5551: Role of YB-1, a regulator of epithelial to mesenchymal transition, in prostate cancer: inhibition by a dietary flavonoid fisetin.  

Science Journals Connector (OSTI)

...family proteins by the dietary compound fisetin in HCT-116 human colon cancer cells...14-18, 2007; Los Angeles, CA 4982 Fisetin, 3,3',4',7-tetrahydroxyflavone...activity. We have previously observed that fisetin inhibits the proliferation of HT-29...

Vaqar Mustafa Adhami; Mohammad Imran Khan; Rahul K. Lall; Imtiaz A. Siddiqui; Deeba N. Syed; Mario Sechi; Shah-Jahan M. Dodwad; Hasan Mukhtar

2013-04-15T23:59:59.000Z

431

Nuclear Janus-Activated Kinase 2/Nuclear Factor 1-C2 Suppresses Tumorigenesis and Epithelial-to-Mesenchymal Transition by Repressing Forkhead Box F1  

Science Journals Connector (OSTI)

...Association for Cancer Research. 1 March 2010 research-article Tumor...Stem Cell Biology Nuclear Janus-Activated...component of the program, other EMT regulators...39). Future research will reveal how...patients with nuclear NF1-C2 staining...

Jeanette Nilsson; Khalil Helou; Anik Kovcs; Pr-Ola Bendahl; Gunnar Bjursell; Mrten Fern; Peter Carlsson; and Marie Kannius-Janson

2010-03-01T23:59:59.000Z

432

Batteries and Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collage of electric cars, plug, battery research lab Collage of electric cars, plug, battery research lab Batteries and Fuel Cells EETD researchers study the basic science and development of advanced batteries and fuel cells for transportation, electric grid storage, and other stationary applications. This research is aimed at developing more environmentally friendly technologies for generating and storing energy, including better batteries and fuel cells. Li-Ion and Other Advanced Battery Technologies Research conducted here on battery technology is aimed at developing low-cost rechargeable advanced electrochemical batteries for both automotive and stationary applications. The goal of fuel cell research is to provide the technologies for the successful commercialization of polymer-electrolyte and solid oxide fuel

433

Fuel Cell Technologies Overview  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

States Energy Advisory Board (STEAB) States Energy Advisory Board (STEAB) Washington, DC Dr. Sunita Satyapal U.S. Department of Energy Fuel Cell Technologies Program Program Manager 3/14/2012 2 | Fuel Cell Technologies Program Source: US DOE 3/19/2013 eere.energy.gov * Introduction - Technology and Market Overview * DOE Program Overview - Mission & Structure - R&D Progress - Demonstration & Deployments * State Activities - Examples of potential opportunities Outline 3 | Fuel Cell Technologies Program Source: US DOE 3/19/2013 eere.energy.gov Fuel cells - convert chemical energy directly into electrical energy, bypassing inefficiencies associated with thermal energy conversion. Available energy is equal to the Gibbs free energy. Combustion Engines - convert chemical energy into thermal energy and

434

Solar cell array interconnects  

DOE Patents [OSTI]

Electrical interconnects are disclosed for solar cells or other electronic components using a silver-silicone paste or a lead-tin (Pb-Sn) no-clean fluxless solder cream, whereby the high breakage of thin (<6 mil thick) solar cells using conventional solder interconnect is eliminated. The interconnects of this invention employs copper strips which are secured to the solar cells by a silver-silicone conductive paste which can be used at room temperature, or by a Pb-Sn solder cream which eliminates undesired residue on the active surfaces of the solar cells. Electrical testing using the interconnects of this invention has shown that no degradation of the interconnects developed under high current testing, while providing a very low contact resistance value. 4 figs.

Carey, P.G.; Thompson, J.B.; Colella, N.J.; Williams, K.A.

1995-11-14T23:59:59.000Z

435

Microbubble cell actuator  

E-Print Network [OSTI]

The field of microsystems technology is rapidly growing, and expanding its horizons to applications in bioengineering. Currently, there are no cell analysis systems that facilitate the collection of dynamic responses for ...

Braff, Rebecca A. (Rebecca Alice)

1999-01-01T23:59:59.000Z

436

Organic solar cell optimizations  

Science Journals Connector (OSTI)

This paper presents recent experimental and theoretical approaches for optimizing organic solar cell efficiencies in both space and energy/time...D/A...) blend film, a corresponding DBAB- block copolymer film ex...

S. Sun; Z. Fan; Y. Wang; J. Haliburton

2005-03-01T23:59:59.000Z

437

Thin film photovoltaic cells  

DOE Patents [OSTI]

A solar cell has as its transparent electrical contact a grid made from a non-noble metal by providing a layer of copper oxide between the transparent electrical contact and the absorber-generator.

Rothwarf, Allen (Philadelphia, PA)

1981-01-01T23:59:59.000Z

438

Solid Oxide Fuel Cells  

Science Journals Connector (OSTI)

A Solid Oxide Fuel Cell (SOFC) is typically composed of two porous electrodes, interposed between an electrolyte made of a particular solid oxide ceramic material. The system originates from the work of Nernst...

Nigel M. Sammes; Roberto Bove; Jakub Pusz

2006-01-01T23:59:59.000Z

439

Photovoltaic solar cell  

DOE Patents [OSTI]

A photovoltaic solar cell for generating electricity from sunlight is disclosed. The photovoltaic solar cell comprises a plurality of spaced-apart point contact junctions formed in a semiconductor body to receive the sunlight and generate the electicity therefrom, the plurality of spaced-apart point contact junctions having a first plurality of regions having a first doping type and a second plurality of regions having a second doping type. In addition, the photovoltaic solar cell comprises a first electrical contact electrically connected to each of the first plurality of regions and a second electrical contact electrically connected to each of the second plurality of regions, as well as a passivation layer covering major surfaces and sidewalls of the photovoltaic solar cell.

Nielson, Gregory N; Okandan, Murat; Cruz-Campa, Jose Luis; Resnick, Paul J

2013-11-26T23:59:59.000Z

440

Photovoltaic solar cell  

DOE Patents [OSTI]

A photovoltaic solar cell for generating electricity from sunlight is disclosed. The photovoltaic solar cell comprises a plurality of spaced-apart point contact junctions formed in a semiconductor body to receive the sunlight and generate the electricity therefrom, the plurality of spaced-apart point contact junctions having a first plurality of regions having a first doping type and a second plurality of regions having a second doping type. In addition, the photovoltaic solar cell comprises a first electrical contact electrically connected to each of the first plurality of regions and a second electrical contact electrically connected to each of the second plurality of regions, as well as a passivation layer covering major surfaces and sidewalls of the photovoltaic solar cell.

Nielson, Gregory N; Cruz-Campa, Jose Luis; Okandan, Murat; Resnick, Paul J

2014-05-20T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Thin silicon solar cells  

SciTech Connect (OSTI)

The silicon-film design achieves high performance by using a dun silicon layer and incorporating light trapping. Optimally designed thin crystalline solar cells (<50 microns thick) have performance advantages over conventional thick devices. The high-performance silicon-film design employs a metallurgical barrier between the low-cost substrate and the thin silicon layer. Light trapping properties of silicon-film on ceramic solar cells are presented and analyzed. Recent advances in process development are described here.

Hall, R.B.; Bacon, C.; DiReda, V.; Ford, D.H.; Ingram, A.E.; Cotter, J.; Hughes-Lampros, T.; Rand, J.A.; Ruffins, T.R.; Barnett, A.M. [Astro Power Inc., Solar Park, Newark, DE (United States)

1992-12-01T23:59:59.000Z

442

Compliant fuel cell system  

DOE Patents [OSTI]

A fuel cell assembly comprising at least one metallic component, at least one ceramic component and a structure disposed between the metallic component and the ceramic component. The structure is configured to have a lower stiffness compared to at least one of the metallic component and the ceramic component, to accommodate a difference in strain between the metallic component and the ceramic component of the fuel cell assembly.

Bourgeois, Richard Scott (Albany, NY); Gudlavalleti, Sauri (Albany, NY)

2009-12-15T23:59:59.000Z

443

Monolithic tandem solar cell  

SciTech Connect (OSTI)

It is an object of the invention to provide a monolithic tandem photovoltaic solar cell which is highly radiation resistant and efficient; in which the energy bandgap of the lower subcell can be tailored for specific applications; solar cell comprising layers of InP and GaInAsP (or GaInAs), where said photovoltaic cell is useful, for example, in space power applications; having an improved power-to-mass ratio; in which subcells are lattice-matches; and are both two terminal and three terminal monolithic tandem photovoltaic solar cells. To achieve the foregoing and other objects and in accordance with the purpose of the present invention, as embodied and broadly described herein, the monolithic tandem photovoltaic solar cell may comprise; (a) an InP substrate having an upper surface; (b) a first photoactive subcell on the upper surface of the InP substrate; wherein the first subcell comprises GaInAs (which could include GaInAsP) and includes a homojunction; and (c) a second photoactive subcell on the first subcell; wherein the second subcell comprises InP and includes a homojunction. The cell is described in detail. 5 figs., 2 tabs.

Wanlass, M.W.

1989-11-03T23:59:59.000Z

444

E-CELL: software environment for whole-cell simulation  

Science Journals Connector (OSTI)

......protein synthesis. The cell would become unable...due to spontaneous degradation. Figure 5 is a trace...ATP in the starving cell. Glucose in the...shortage of glucose to fuel glycolysis arrests...mRNA, in which the cell was starved at 1000...transcription and degradation. When the cell runs......

M Tomita; K Hashimoto; K Takahashi; TS Shimizu; Y Matsuzaki; F Miyoshi; K Saito; S Tanida; K Yugi; JC Venter; CA Hutchison; 3rd

1999-01-01T23:59:59.000Z

445

Cell Component Accelerated Stress Test Protocols for PEM Fuel Cells  

Broader source: Energy.gov [DOE]

Accelerated Stress Test Protocols for PEM Fuel Cells, Electrocatalysts, Supports, Membranes, and Membrane Electrode Assemblies

446

Single Cell Motion in Aggregates of Embryonic Cells  

Science Journals Connector (OSTI)

We investigate the motion of individual pigmented retinal cells in aggregates of neural retinal cells obtained from chicken embryos. Individual cells in aggregates move randomly in the absence of chemical or adhesion gradients: the power spectrum of position and velocity versus time correspond to Brownian motion and the velocities are uncorrelated in time. Thus a quasithermal model of cell migration like the extended Potts model is appropriate. We also measure cell diffusivity and the cell velocity distribution. The results support the idea that collections of embryonic cells behave as liquids with membrane fluctuations playing the role of temperature.

Jos C. M. Mombach and James A. Glazier

1996-04-15T23:59:59.000Z

447

The Sensitivity of MCF10A Breast Epithelial Cells to Alkylating Drugs is Enhanced by the Inhibition of O6-Methylguanine-DNA Methyltransferase Transcription with a Synthetic Double Strand DNA Oligonucleotide  

Science Journals Connector (OSTI)

Cytoxicity of alkylating chemotherapeutic drugs is affected by the cellular content of the enzyme O6-methylguanine-DNA methyl transferase (MGMT). Since high levels ... of the enzyme confer the efficient repair of...

Laura Mariani; Antonella Piccirilli; Lorenzo Citti

2002-06-01T23:59:59.000Z

448

Consistency of quantitative multiplexed - methylation specific PCR (QM-MSP) performed on breast epithelial cells acquired by random periareolar fine needle aspiration (RPFNA) of women at high risk for development of breast cancer.  

Science Journals Connector (OSTI)

...women with persistent high-risk HPV infection and low-grade...Polyphenon E was shown to be acceptable, safe and well tolerated...defined as negative for high risk HPV and normal histopathology...clearance of persistent high risk HPV and related CIN 1. Further...

Bruce Kimler; Mary Jo Fackler; Trina Metheny; Teresa Phillips; Saraswati Sukumar; and Carol Fabian

2007-05-01T23:59:59.000Z

449

NANOCOMPOSITE ENABLED SENSITIZED SOLAR CELL  

E-Print Network [OSTI]

solar cell The history of dye sensitization dates back to the photosensitization phenomenon by organic

Phuyal, Dibya

2012-01-01T23:59:59.000Z

450

An advanced fuel cell simulator  

E-Print Network [OSTI]

of Fuel Cells ...................... 4 D. Fuel Cell Power Plant ..................... 4 E. Challenges in Fuel Cell Development ............ 5 F. Previous Work ......................... 6 G. Solar Array Simulators .................... 8 H. Battery... ............................. 54 28 Under-voltage Fault ........................... 55 1 CHAPTER I INTRODUCTION The depleting fossil fuel resources and increasing pollution are leading to the research and development of alternate energy generation techniques like fuel cells...

Acharya, Prabha Ramchandra

2005-11-01T23:59:59.000Z

451

Hydrogen & Fuel Cells Program Overview  

E-Print Network [OSTI]

Hydrogen & Fuel Cells Program Overview Dr. Sunita Satyapal Program Manager Hydrogen and Fuel Cells Program U.S. Department of Energy Hydrogen + Fuel Cells 2011 International Conference and Exhibition Vancouver, Canada May 17, 2011 #12;Enable widespread commercialization of hydrogen and fuel cell

452

Breakthrough Vehicle Development - Fuel Cells  

Fuel Cell Technologies Publication and Product Library (EERE)

Document describing research and development program for fuel cell power systems for transportation applications.

453

Fuel Cell Technologies Office: Glossary  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Glossary to someone by Glossary to someone by E-mail Share Fuel Cell Technologies Office: Glossary on Facebook Tweet about Fuel Cell Technologies Office: Glossary on Twitter Bookmark Fuel Cell Technologies Office: Glossary on Google Bookmark Fuel Cell Technologies Office: Glossary on Delicious Rank Fuel Cell Technologies Office: Glossary on Digg Find More places to share Fuel Cell Technologies Office: Glossary on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Program Presentations Multimedia Conferences & Meetings Webinars Data Records Databases Glossary Quick Links Hydrogen Production Hydrogen Delivery Hydrogen Storage Fuel Cells Technology Validation Manufacturing Codes & Standards Education Systems Analysis Contacts Glossary

454

Fuel Cell Technologies Office: Presentations  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Presentations to Presentations to someone by E-mail Share Fuel Cell Technologies Office: Presentations on Facebook Tweet about Fuel Cell Technologies Office: Presentations on Twitter Bookmark Fuel Cell Technologies Office: Presentations on Google Bookmark Fuel Cell Technologies Office: Presentations on Delicious Rank Fuel Cell Technologies Office: Presentations on Digg Find More places to share Fuel Cell Technologies Office: Presentations on AddThis.com... Publications Program Publications Technical Publications Educational Publications Newsletter Program Presentations Multimedia Conferences & Meetings Annual Merit Review Proceedings Workshop & Meeting Proceedings Webinars Data Records Databases Glossary Quick Links Hydrogen Production Hydrogen Delivery Hydrogen Storage Fuel Cells

455

Fuel Cells at NASCAR | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Cells at NASCAR Fuel Cells at NASCAR Download presentation slides from the DOE Fuel Cell Technologies Office webinar "Fuel Cells at NASCAR" held on April 17, 2014. Fuel Cells at...

456

Sulphoraphane, a naturally occurring isothiocyanate induces apoptosis in breast cancer cells by targeting heat shock proteins  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer HSPs (27, 70 and 90) and HSF1 are overexpressed in MCF-7 and MDA-MB-231 cells. Black-Right-Pointing-Pointer Sulphoraphane, a natural isothiocyanate inhibited HSPs and HSF1 expressions. Black-Right-Pointing-Pointer Inhibition of HSPs and HSF1 lead to regulation of apoptotic proteins. Black-Right-Pointing-Pointer Alteration of apoptotic proteins activate of caspases particularly caspase 3 and 9 leading to induction of apoptosis. Black-Right-Pointing-Pointer Alteration of apoptotic proteins induce caspases leading to induction of apoptosis. -- Abstract: Heat shock proteins (HSPs) are involved in protein folding, aggregation, transport and/or stabilization by acting as a molecular chaperone, leading to inhibition of apoptosis by both caspase dependent and/or independent pathways. HSPs are overexpressed in a wide range of human cancers and are implicated in tumor cell proliferation, differentiation, invasion and metastasis. HSPs particularly 27, 70, 90 and the transcription factor heat shock factor1 (HSF1) play key roles in the etiology of breast cancer and can be considered as potential therapeutic target. The present study was designed to investigate the role of sulphoraphane, a natural isothiocyanate on HSPs (27, 70, 90) and HSF1 in two different breast cancer cell lines MCF-7 and MDA-MB-231 cells expressing wild type and mutated p53 respectively, vis-a-vis in normal breast epithelial cell line MCF-12F. It was furthermore investigated whether modulation of HSPs and HSF1 could induce apoptosis in these cells by altering the expressions of p53, p21 and some apoptotic proteins like Bcl-2, Bax, Bid, Bad, Apaf-1 and AIF. Sulphoraphane was found to down-regulate the expressions of HSP70, 90 and HSF1, though the effect on HSP27 was not pronounced. Consequences of HSP inhibition was upregulation of p21 irrespective of p53 status. Bax, Bad, Apaf-1, AIF were upregulated followed by down-regulation of Bcl-2 and this effect was prominent in MCF-7 than in MDA-MB-231. However, very little change in the expression of Bid was observed. Alteration in Bcl-2 Bax ratio resulted in the release of cytochrome c from mitochondria and activation of caspases 3 and 9 which are in agreement with apoptotic index values. Sulphoraphane therefore can be regarded as a potent inducer of apoptosis due to HSP modulation in breast cancer cells.

Sarkar, Ruma; Mukherjee, Sutapa [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India); Biswas, Jaydip [Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India); Roy, Madhumita, E-mail: mitacnci@yahoo.co.in [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)

2012-10-12T23:59:59.000Z

457

Theoretical study of the competition between cell-cell and cell-matrix adhesions  

Science Journals Connector (OSTI)

Adhesions between neighboring cells or between cells and their surrounding tissue/matrix play a crucial role in a wide range of biological processes. In order to investigate the competitive mechanisms between cell-cell and cell-matrix adhesions, we here develop a theoretical framework for multiple interacting cells lying on a planar matrix coated with distributed ligands. This model allows us to study, from the viewpoints of energy and statistics, the effects of such physical mechanisms as binding energy of bonds, nonspecific interactions, elastic deformation of cell membranes, and mixing entropy. Our calculations show that cell-matrix adhesion cannot occur when the ligand density on the matrix is lower than a threshold value, and cell-cell adhesion does not happen for a high ligand density. Glycocalyx repulsion plays a more important role in cell-matrix adhesion than in cell-cell adhesion. In addition, it is found that the cell-cell adhesion density decreases as the number of cells increases.

Guang-Kui Xu; Xi-Qiao Feng; Hong-Ping Zhao; Bo Li

2009-07-27T23:59:59.000Z

458

Cell Phone Detection Techniques  

SciTech Connect (OSTI)

A team composed of Rick Pratt, Dave Puczyki, Kyle Bunch, Ryan Slaugh, Morris Good, and Doug McMakin teamed together to attempt to exploit cellular telephone features and detect if a person was carrying a cellular telephone into a Limited Area. The cell phones electromagnetic properties were measured, analyzed, and tested in over 10 different ways to determine if an exploitable signature exists. The method that appears to have the most potential for success without adding an external tag is to measure the RF spectrum, not in the cell phone band, but between 240 and 400MHz. Figures 1- 7 show the detected signal levels from cell phones from three different manufacturers.

Pratt, Richard M.; Bunch, Kyle J.; Puzycki, David J.; Slaugh, Ryan W.; Good, Morris S.; McMakin, Douglas L.

2007-10-01T23:59:59.000Z

459

Separators for electrochemical cells  

DOE Patents [OSTI]

Provided are separators for use in an electrochemical cell comprising (a) an inorganic oxide and (b) an organic polymer, wherein the inorganic oxide comprises organic substituents. Preferably, the inorganic oxide comprises an hydrated aluminum oxide of the formula Al.sub.2O.sub.3.xH.sub.2O, wherein x is less than 1.0, and wherein the hydrated aluminum oxide comprises organic substituents, preferably comprising a reaction product of a multifunctional monomer and/or organic carbonate with an aluminum oxide, such as pseudo-boehmite and an aluminum oxide. Also provided are electrochemical cells comprising such separators.

Carlson, Steven Allen; Anakor, Ifenna Kingsley

2014-11-11T23:59:59.000Z

460

Superlattice cascade solar cell  

SciTech Connect (OSTI)

This paper reports progress toward realization of a new cascade solar cell structure whose chief advantages over other present concepts are: use of silicon for the substrate and low bandgap cell; avoidance of the necessity of lattice matching; and incorporation of a GaAs/GaP superlattice to enhance efficiency and provide a low-resistance connecting junction. Details of the design and operation of an OMCVD system for growing this structure are presented. Results of experiments to optimize layer thickness, compositional uniformity, and surface morphology are described.

Wanlass, M.W.; Blakeslee, A.E.

1982-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Monolithic tandem solar cell  

SciTech Connect (OSTI)

This patent describes a single-crystal, monolithic, tandem, photovoltaic solar cell which includes an InP substrate having an upper and lower surfaces, a first photoactive subcell on the upper surface of the InP substrate, and a second photoactive subcell on the first subcell. The first photovoltaic subcell is GaInAsP of defined composition. The second subcell is InP. The two subcells are lattice matched. The solar cell can be provided as a two- terminal device or a three-terminal device.

Wanlass, M.W.

1991-05-28T23:59:59.000Z

462

Cylindrical electric cell  

SciTech Connect (OSTI)

The invention relates to a cylindrical electric cell in which the negative electrode (8) is a sheet of lithium wound round a current collector (1) which is elastically deformable and is in the form of a cylinder whose directrix is a substantially circular curve with two distinct ends. In accordance with the invention, the two portions of cylinder close to said ends are cut into matching shapes so that they fit into each other, the protruding parts (2) of one portion being accomodated in the recessed parts (3) of the other portion. Application to electric cells whose positive active material is liquid.

Gelin, G.

1980-12-02T23:59:59.000Z

463

Gene expression profiling reveals novel regulation by bisphenol-A in estrogen receptor-{alpha}-positive human cells  

SciTech Connect (OSTI)

Bisphenol-A (BPA) shows proliferative actions in uterus and mammary glands and may influence the development of male and female reproductive tracts in utero or during early postnatal life. Because of its ability to function as an estrogen receptor (ER) agonist, BPA has the potential to disrupt normal endocrine signaling through regulation of ER target genes. Some genes are regulated by both estradiol (E2) and BPA, but those exclusive to either agent have not been described. Using a yeast strain incorporating a vitellogenin A2 ERE-LacZ reporter gene into the genome, we found that BPA induced expression of the reporter in colonies transformed with the ER{alpha} expression plasmid, illustrating BPA-mediated regulation within a chromatin context. Additionally, a reporter gene transiently transfected into the endometrial cancer (Ishikawa) cell line also showed BPA activity, although at 100-fold less potency than E2. To compare global gene expression in response to BPA and E2, we used a variant of the MCF-7 breast cancer cell line stably expressing HA-tagged ER{alpha}. Cultures were treated for 3 h with an ethanol vehicle, E2 (10{sup -8} M), or BPA (10{sup -6} M), followed by isolation of RNA and microarray analysis with the human U95A probe array (Affymetrix, Santa Clara, CA, USA). More than 300 genes were changed 2-fold or more by either or both agents, with roughly half being up-regulated and half down-regulated. A number of growth- and development-related genes, such as HOXC1 and C6, Wnt5A, Frizzled, TGF{beta}-2, and STAT inhibitor 2, were found to be affected exclusively by BPA. We used quantitative real-time PCR to verify regulation of the HOXC6 gene, which showed decreased expression of approximately 2.5-fold by BPA. These results reveal novel effects by BPA and E2, raising interesting possibilities regarding the role of endocrine disruptors in sexual development.

Singleton, David W. [Department of Cell Biology, Neurobiology, and Anatomy, Vontz Center for Molecular Studies, University of Cincinnati, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Feng, Yuxin [Department of Cell Biology, Neurobiology, and Anatomy, Vontz Center for Molecular Studies, University of Cincinnati, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Yang, Jun [Department of Cell Biology, Neurobiology, and Anatomy, Vontz Center for Molecular Studies, University of Cincinnati, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Puga, Alvaro [Department of Environmental Health, University of Cincinnati, Cincinnati, OH (United States); Lee, Adrian V. [Baylor College of Medicine, Houston, TX (United States); Khan, Sohaib A. [Department of Cell Biology, Neurobiology, and Anatomy, Vontz Center for Molecular Studies, University of Cincinnati, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States)]. E-mail: sohaib.khan@uc.edu

2006-01-15T23:59:59.000Z

464

Solar Cells: Spin-Cast Bulk Heterojunction Solar Cells: A Dynamical...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Solar Cells: Spin-Cast Bulk Heterojunction Solar Cells: A Dynamical Investigation Solar Cells: Spin-Cast Bulk Heterojunction Solar Cells: A Dynamical Investigation Print Wednesday,...

465

Porcine adenovirus serotype 3 internalization is independent of CAR and ?v?3 or ?v?5 integrin  

Science Journals Connector (OSTI)

Nonhuman adenoviruses including porcine adenovirus serotype 3 (PAd3) are emerging vectors for gene delivery. \\{PAd3\\} efficiently transduces human and murine cells in culture, and circumvents preexisting humoral immunity in humans. The coxsackievirusadenovirus receptor (CAR) serves as a primary receptor and ?v?3 or ?v?5 integrin as a secondary receptor for several human adenovirus (HAd) subtypes including HAd5. In this study, we deduced the role of CAR, ?v?3 or ?v?5 integrin in \\{PAd3\\} internalization. Transduction experiments were conducted in human mammary epithelial (MCF-10A) cells using replication-defective PAd-GFP (PAd3 vector expressing green fluorescent protein [GFP]) and HAd-GFP (HAd5 vector expressing GFP). MCF-10A cells were treated with or without anti-human CAR, or anti-?v?3 or anti-?v?5 integrin antibodies prior to infection with HAd-GFP or PAd-GFP. Significant (P CAR- as well as ?v?3 or ?v?5 integrin-independent and the primary receptor for \\{HAd5\\} and \\{PAd3\\} were distinct. CAR- and ?v?3 or ?v?5 integrin-independent entry of \\{PAd3\\} vectors may have implications in targeting cell types that are not efficiently transduced by other adenoviral vectors.

Dinesh S. Bangari; Suresh K. Mittal

2005-01-01T23:59:59.000Z

466

MicroRNA expression in canine mammary cancer  

E-Print Network [OSTI]

to act as both tumor suppressors and oncogenes in several different cancers, expression patterns of ten miRNAs (miR-15a, miR-16, miR-17-5p, miR-21, miR-29b, miR-125b, miR-145, miR-155, miR-181b, let-7f) known to be associated with human breast cancer were...

Boggs, Rene' Michelle

2008-10-10T23:59:59.000Z

467

Effect of Exercise on the Induction of Mammary Carcinogenesis  

Science Journals Connector (OSTI)

...010 0.010 0.100 731 The Energy Expenditure of Rats Bearing...that rats are of comparable corn position if the weight of the...nitro.. Mmir@iiet al. "Energy Expenditure of Tumorous Rats...to assume that the principal energy-producing foodstuffs, i...

Henry J. Thompson; Anne M. Ronan; Karen A. Ritacco; Anthony R. Tagliaferro; and L. David Meeker

1988-05-15T23:59:59.000Z

468

Mammary Gland Development, Reproductive History, and Breast Cancer Risk  

Science Journals Connector (OSTI)

...Development, Reproductive History, and Breast Cancer...Molecular and Cellular Engineering, Stellar-Chance...Molecular and Cellular Engineering [L. A. C., C...Development, Reproductive History, and Breast Cancer...Molecular and Cellular Engineering [L. A. C., C...

Lewis A. Chodosh; Celina M. D'Cruz; Heather Perry Gardner; Seung I. Ha; Sandra T. Marquis; Jayant V. Rajan; Douglas B. Stairs; James Y. Wang; and Man Wang

1999-04-01T23:59:59.000Z

469

Neutron-induced Mammary Neoplasms in the Rat  

Science Journals Connector (OSTI)

...female Sprague-Dawley rat. Other workers have established a linear relationship...female Sprague-Dawley rat. Other workers have established a linear relationship...irradiated several groups of rats at the Oak Ridge Health Physics Reactor to a single...

Howard H. Vogel, Jr. and Robert Zaldvar

1972-05-01T23:59:59.000Z

470

Regression of Human Metastatic Mammary Cancer Induced by 3-Methylcholanthrene  

Science Journals Connector (OSTI)

...Israel and Egypt! We do have the usual health hazard warnings, ofcourse. Fig. 8 is...paraphrased, says, oeWarning. The Health Officer Has Determined That Wading is Dangerous To Your Health. Epidemiology of Survival What happens...

Charles Huggins and Jack D. McCarthy

1957-11-01T23:59:59.000Z

471

Regression of Human Metastatic Mammary Cancer Induced by 3-Methylcholanthrene  

Science Journals Connector (OSTI)

...question of the relative health risk of LN-SLT products...products to be far less dangerous than conventional cigarettes...government officials or public health experts to characterize...products as comparably dangerous with cigarette smoking...in tobacco-related health risks in an exciting...

Charles Huggins and Jack D. McCarthy

1957-11-01T23:59:59.000Z

472

Sequencing the transcriptome of milk production: milk trumps mammary tissue  

E-Print Network [OSTI]

important for milk lipid secretion. J Biol Chem 53. Russellin milk lipid formation and secretion. Trends Endocrinol

2013-01-01T23:59:59.000Z

473

Lysolecithin and Cell Fusion  

Science Journals Connector (OSTI)

... INTEREST in the fusion of biological membranes has recently been stimulated by investigations on the biochemistry of secretion1, ... of membranes in the lysosomal vacuolar system2 and, in particular, by work on the fusion of cells that is induced by viruses3'4. For C3ll ...

A. R. POOLE; J. I. HOWELL; J. A. LUCY

1970-08-22T23:59:59.000Z

474

Photo-Electric Cells  

Science Journals Connector (OSTI)

... T. for the grounds of his most serious criticism of our volume on Photo-electric Cells reviewed in NATURE of July 19, p. 90. He says that ... reviewed in NATURE of July 19, p. 90. He says that the theory that photo-electrons are the free electrons of the metal is not one which is generally ...

NORMAN R. CAMPBELL; DOROTHY RITCHIE

1930-08-30T23:59:59.000Z

475

Thin film photovoltaic cell  

DOE Patents [OSTI]

A thin film photovoltaic cell having a transparent electrical contact and an opaque electrical contact with a pair of semiconductors therebetween includes utilizing one of the electrical contacts as a substrate and wherein the inner surface thereof is modified by microroughening while being macro-planar.

Meakin, John D. (Newark, DE); Bragagnolo, Julio (Newark, DE)

1982-01-01T23:59:59.000Z

476

Solar Cell Simulation  

K-12 Energy Lesson Plans and Activities Web site (EERE)

Students model the flow of energy from the sun as it enters a photovoltaic cell, moves along a wire and powers a load. The game-like atmosphere involves the younger students and helps them understand the continuous nature of the flow of energy. For a related lesson, please see the activity Solar Powered System (PDF 430 KB).

477

Introgression & mapping Fiber cell  

E-Print Network [OSTI]

Germplasm Introgression Genomics & mapping Fiber cell initiation Radiation hybrid (RH) mapping and breeding. Research activities commonly include plant breeding, genetics, genomics, cytogenetics, molecular methods. (C, S) · Contribute uniquely to genomics and its relevance to genetic improvement (C,S) · Harness

478

Fuel Cell Technologies Office: Joint Fuel Cell Bus Workshop  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cell Bus Workshop Fuel Cell Bus Workshop The U.S. Department of Energy (DOE) and the U.S. Department of Transportation (DOT) held a Fuel Cell Bus Workshop on June 7, 2010 in Washington, D.C. in conjunction with the DOE Hydrogen and Fuel Cell Program Annual Merit Review. The workshop plenary and breakout session brought together technical experts from industry, end users, academia, DOE national laboratories, and other government agencies to address the status and technology needs of fuel cell powered buses. Meeting Summary Joint Fuel Cell Bus Workshop Summary Report Presentations Fuel Cell Bus Workshop Overview & Purpose, Dimitrios Papageorgopoulos, DOE Users Perspective on Advanced Fuel Cell Bus Technology, Nico Bouwkamp, CaFCP and Leslie Eudy, NREL Progress and Challenges for PEM Transit Fleet Applications, Tom Madden, UTC Power, LLC

479

Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities  

Broader source: Energy.gov (indexed) [DOE]

& & Renewable Energy Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities Pete Devlin Fuel Cell Technologies Program United States Department of Energy Federal Utility Partnership Working Group April 14 th , 2010 2 * DOE Fuel Cell Market Transformation Overview * Overview of CHP Concept * Stationary Fuel Cells for CHP Applications * Partnering and Financing (Sam Logan) * Example Project Outline 3 Fuel Cells: Addressing Energy Challenges Energy Efficiency and Resource Diversity  Fuel cells offer a highly efficient way to use diverse fuels and energy sources. Greenhouse Gas Emissions and Air Pollution:  Fuel cells can be powered by emissions-free fuels that are produced from clean, domestic resources. Stationary Power (including CHP & backup power)

480

Diagnostic Studies on Li-Battery Cells and Cell Components  

Broader source: Energy.gov [DOE]

2011 DOE Hydrogen and Fuel Cells Program, and Vehicle Technologies Program Annual Merit Review and Peer Evaluation

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481

Diagnostic Studies on Lithium Battery Cells and Cell Components  

Broader source: Energy.gov [DOE]

2012 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

482

Fuel Cell Handbook, Fourth Edition  

SciTech Connect (OSTI)

Robust progress has been made in fuel cell technology since the previous edition of the Fuel Cell Handbook was published in January 1994. This Handbook provides a foundation in fuel cells for persons wanting a better understanding of the technology, its benefits, and the systems issues that influence its application. Trends in technology are discussed, including next-generation concepts that promise ultra high efficiency and low cost, while providing exceptionally clean power plant systems. Section 1 summarizes fuel cell progress since the last edition and includes existing power plant nameplate data. Section 2 addresses the thermodynamics of fuel cells to provide an understanding of fuel cell operation at two levels (basic and advanced). Sections 3 through 6 describe the four major fuel cell types and their performance based on cell operating conditions. The section on polymer electrolyte membrane fuel cells has been added to reflect their emergence as a significant fuel cell technology. Phosphoric acid, molten carbonate, and solid oxide fuel cell technology description sections have been updated from the previous edition. New information indicates that manufacturers have stayed with proven cell designs, focusing instead on advancing the system surrounding the fuel cell to lower life cycle costs. Section 7, Fuel Cell Systems, has been significantly revised to characterize near-term and next-generation fuel cell power plant systems at a conceptual level of detail. Section 8 provides examples of practical fuel cell system calculations. A list of fuel cell URLs is included in the Appendix. A new index assists the reader in locating specific information quickly.

Stauffer, D.B; Hirschenhofer, J.H.; Klett, M.G.; Engleman, R.R.

1998-11-01T23:59:59.000Z

483

EE580 Solar Cells Todd J. Kaiser  

E-Print Network [OSTI]

7/21/2010 1 EE580 ­ Solar Cells Todd J. Kaiser · Lecture 06 · Solar Cell Materials & Structures 1Montana State University: Solar Cells Lecture 6: Solar Cells Solar Cell Technologies · A) Crystalline Silicon · B) Thin Film · C) Group III-IV Cells 2Montana State University: Solar Cells Lecture 6: Solar

Kaiser, Todd J.

484

Prolonged effect of fluid flow stress on the proliferative activity of mesothelial cells after abrupt discontinuation of fluid streaming  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Late-onset peritoneal fibrosis leading to EPS remains to be elucidated. Black-Right-Pointing-Pointer Fluid streaming is a potent factor for peritoneal fibrosis in PD. Black-Right-Pointing-Pointer We focused on the prolonged effect of fluid streaming on mesothelial cell kinetics. Black-Right-Pointing-Pointer A history of fluid streaming exposure promoted mesothelial proliferative activity. Black-Right-Pointing-Pointer We have thus identified a potent new factor for late-onset peritoneal fibrosis. -- Abstract: Encapsulating peritoneal sclerosis (EPS) often develops after transfer to hemodialysis and transplantation. Both termination of peritoneal dialysis (PD) and transplantation-related factors are risks implicated in post-PD development of EPS, but the precise mechanism of this late-onset peritoneal fibrosis remains to be elucidated. We previously demonstrated that fluid flow stress induced mesothelial proliferation and epithelial-mesenchymal transition via mitogen-activated protein kinase (MAPK) signaling. Therefore, we speculated that the prolonged bioactive effect of fluid flow stress may affect mesothelial cell kinetics after cessation of fluid streaming. To investigate how long mesothelial cells stay under the bioactive effect brought on by fluid flow stress after removal of the stress, we initially cultured mesothelial cells under fluid flow stress and then cultured the cells under static conditions. Mesothelial cells exposed to fluid flow stress for a certain time showed significantly high proliferative activity compared with static conditions after stoppage of fluid streaming. The expression levels of protein phosphatase 2A, which dephosphorylates MAPK, in mesothelial cells changed with time and showed a biphasic pattern that was dependent on the duration of exposure to fluid flow stress. There were no differences in the fluid flow stress-related bioactive effects on mesothelial cells once a certain time had passed. The present findings show that fluid flow stress exerts a prolonged bioactive effect on mesothelial cells after termination of fluid streaming. These findings support the hypothesis that a history of PD for a certain period could serve as a trigger of EPS after stoppage of PD.

Aoki, Shigehisa, E-mail: aokis@cc.saga-u.ac.jp [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan); Ikeda, Satoshi [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan); Takezawa, Toshiaki [Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki (Japan)] [Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki (Japan); Kishi, Tomoya [Department of Internal Medicine, Saga University, Saga (Japan)] [Department of Internal Medicine, Saga University, Saga (Japan); Makino, Junichi [Makino Clinic, Saga (Japan)] [Makino Clinic, Saga (Japan); Uchihashi, Kazuyoshi; Matsunobu, Aki [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan); Noguchi, Mitsuru [Department of Urology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Urology, Faculty of Medicine, Saga University, Saga (Japan); Sugihara, Hajime [Department of Physical Therapy, International University of Health and Welfare, Fukuoka (Japan)] [Department of Physical Therapy, International University of Health and Welfare, Fukuoka (Japan); Toda, Shuji [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)

2011-12-16T23:59:59.000Z

485

Hormonally Regulated Programmed Cell Death in Barley Aleurone Cells  

Science Journals Connector (OSTI)

...than for GA-treated cells, and rarely does a single large vacuole fill the cell ( 6 ). Degradation of nuclear DNA is delayed...10 ), and the rate of cell death is considerably...result in an inability to fuel the plasma membrane proton...

Paul C. Bethke; Jennifer E. Lonsdale; Angelika Fath; Russell L. Jones

486

Meteorological Effects on Solar Cells  

Science Journals Connector (OSTI)

30 June 1977 research-article Meteorological Effects on Solar Cells J. R. Mallinson P. T. Landsberg The effect of different meteorological conditions on solar cell outputs has been investigated...

1977-01-01T23:59:59.000Z

487

Acetate metabolism in cancer cells  

E-Print Network [OSTI]

Macromolecule biosynthesis is required to duplicate cell components and support proliferation. Studies examining the nutrients used by cancer cells have focused on the contribution of glucose and glutamine carbon for ...

Hosios, Aaron M

2014-01-01T23:59:59.000Z

488

Fuel Cell Kickoff Meeting Agenda  

Broader source: Energy.gov (indexed) [DOE]

3:40 Aligned Carbon Nanotube-Based MEA and PEMFC D-J Liu, ANL 4:00 Light Weight Low Cost PEM Fuel Cell Stacks J. Wainright, CWRU 4:20 Adaptive Stack with Subdivided Cells for...

489

Air Liquide- Biogas & Fuel Cells  

Broader source: Energy.gov [DOE]

Presentation about Air Liquide's biogas technologies and integration with fuel cells. Presented by Charlie Anderson, Air Liquide, at the NREL/DOE Biogas and Fuel Cells Workshop held June 11-13, 2012, in Golden, Colorado.

490

DOE Hydrogen & Fuel Cell Overview  

Broader source: Energy.gov (indexed) [DOE]

Natural Gas Power Heat + Cooling Electricity Cooling Natural Gas Natural Gas or Biogas Fuel Cell H Excess power generated by the fuel cell is fed to the grid National...

491

Alkaline Membrane Fuel Cell Workshop  

Broader source: Energy.gov [DOE]

A workshop on alkaline membrane fuel cells (AMFC) was held May 8-9, 2011, before the 2011 Hydrogen and Fuel Cells Annual Merit Review, at Crystal Gateway Marriott in Arlington, Virginia.

492

Organic Solar Cells A Survey  

Science Journals Connector (OSTI)

The objective of this study was to examine in some detail the question of efficient organic solar cells. The scientific-technical situation concerning organic solar cells is highly confusing and unsatisfactory. I...

Dieter Bonnet; Jrgen Volkheimer

1991-01-01T23:59:59.000Z

493

2009 Fuel Cell Market Report  

Fuel Cell Technologies Publication and Product Library (EERE)

Fuel cells are electrochemical devices that combine hydrogen and oxygen to produce electricity, water, and heat. Unlike batteries, fuel cells continuously generate electricity, as long as a source of

494

Distinct Luminal-Type Mammary Carcinomas Arise from Orthotopic Trp53-Null Mammary Transplantation of Juvenile versus Adult Mice  

Science Journals Connector (OSTI)

...Program, funding from the Department of Energy, Office of Biological and Environmental...1049-56. 2. Rakha EA , El-Sayed ME, Green AR, Lee AH, Robertson JF Ellis IO.Prognostic...Elsheikh SE, Aleskandarany MA, Habashi HO, Green AR, Powe DG, et alTriple-negative breast...

David H. Nguyen; Haoxu Ouyang; Jian-Hua Mao; Lynn Hlatky; and Mary Helen Barcellos-Hoff

2014-12-01T23:59:59.000Z

495

Phosphorus Metabolism in Resting and Pregnancy-stimulated Mammary Glands and in Spontaneous Mammary Carcinomas of Mice  

Science Journals Connector (OSTI)

...basis of the findings of other workers, it was concluded that this increased...experiments was furnished by the Oak Ridge National Laboratory, Oak Ridge, Tenn. 4 These values...suggested by Schulman and his co-workers (17, 18), has been used to...

S. Albert; R. M. Johnson; and Marjorie S. Cohan

1951-10-01T23:59:59.000Z

496

Phosphorus Metabolism in Resting and Pregnancy-stimulated Mammary Glands and in Spontaneous Mammary Carcinomas of Mice  

Science Journals Connector (OSTI)

...April 16, 1950. S. Albert...48 hours after injection...of other workers, it was...48 hours after injection...furnished by the Oak Ridge National Laboratory, Oak Ridge, Tenn...least 10 days after delivery...April 16, 1950. 1 For the...

S. Albert; R. M. Johnson; and Marjorie S. Cohan

1951-10-01T23:59:59.000Z

497

Fabrication and Characterization of Organic Solar Cells  

E-Print Network [OSTI]

processablepolymerphotovoltaiccellsbyself?organizationPhotodiodes, and Photovoltaic Cells. AppliedPhysicsF, Heeger AJ. Polymer Photovoltaic Cells ? Enhanced

Yengel, Emre

2010-01-01T23:59:59.000Z

498

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

treatment, sedi- ment or marine fuel cells for fieldmicrobial fuel cells demonstrating marine (left) and soil (1]. Sediment and Marine Microbial fuel cells can also

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

499

Fuel Cells News | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Fuel Cells News Fuel Cells News October 16, 2014 Webinar October 21: Opportunities for Wide Bandgap Semiconductor Power Electronics for Hydrogen and Fuel Cell Applications The...

500

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

Model of hydrogen fuel cell kinetic losses includingschematic of typical hydrogen fuel cell performancephase factors on hydrogen fuel cell theoretical efficiency,

Parra, Erika Andrea

2010-01-01T23:59:59.000Z