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1

Epimorphin Functions as a Key Morphoregulator for Mammary Epithelial Cells  

SciTech Connect (OSTI)

Hepatocyte growth factor (HGF) and EGF have been reported to promote branching morphogenesis of mammary epithelial cells. We now show that it is epimorphin that is primarily responsible for this phenomenon. In vivo, epimorphin was detected in the stromal compartment but not in lumenal epithelial cells of the mammary gland; in culture, however, a subpopulation of mammary epithelial cells produced significant amounts of epimorphin. When epimorphin-expressing epithelial cell clones were cultured in collagen gels they displayed branching morphogenesis in the presence of HGF, EGF, keratinocyte growth factor, or fibroblast growth factor, a process that was inhibited by anti-epimorphin but not anti-HGF antibodies. The branch length, however, was roughly proportional to the ability of the factors to induce growth. Accordingly, epimorphin-negative epithelial cells simply grew in a cluster in response to the growth factors and failed to branch. When recombinant epimorphin was added to these collagen gels, epimorphin-negative cells underwent branching morphogenesis. The mode of action of epimorphin on morphogenesis of the gland, however, was dependent on how it was presented to the mammary cells. If epimorphin was overexpressed in epimorphin-negative epithelial cells under regulation of an inducible promoter or was allowed to coat the surface of each epithelial cell in a nonpolar fashion, the cells formed globular, alveoli-like structures with a large central lumen instead of branching ducts. This process was enhanced also by addition of HGF, EGF, or other growth factors and was inhibited by epimorphin antibodies. These results suggest that epimorphin is the primary morphogen in the mammary gland but that growth factors are necessary to achieve the appropriate cell numbers for the resulting morphogenesis to be visualized.

Hirai, H.; Lochter, A.; Galosy, S.; Koshida, S.; Niwa, S.; Bissell, M.J.

1997-10-13T23:59:59.000Z

2

Milk lipid and protein traffic in mammary epithelial cells: joint and independent pathways  

E-Print Network [OSTI]

of prolactin and oxytocin on lipid and protein secretion suggest that these processes are coupled and co is not strictly required. milk lipids / mammary epithelial cells / secretion Résumé -- Transport des lipides et biologists, dairy scientists and technologists: Are the intracellular secretion processes of lipids

Boyer, Edmond

3

Trichostatin A inhibits beta-casein expression in mammary epithelial cells  

SciTech Connect (OSTI)

Many aspects of cellular behavior are affected by information derived from association of the extracellular matrix (ECM) and with cell membrane receptors. When cultured in the presence of laminin-containing ECM and prolactin (Prl), normal mammary epithelial cells express the milk protein beta-casein. Previously, we defined the minimal ECM- and Prl-responsive enhancer element BCE-1 from the upstream region of the beta-casein gene. We also found that BCE-1 was only active when stably integrated into chromatin, and that trichostatin A (TSA), a reagent that leads to alterations in chromatin structure, was able to activate the integrated enhancer element. We now show that endogenous b-casein gene, which is controlled by a genetic assembly that is highly similar to that of BCE-1 and which is also activated by incubation in ECM and Prl, is instead inhibited by TSA. We provide evidence that the differing response of b-casein and BCE-1 to TSA is neither due to an unusual effect of TSA on mammary epithelial cells, nor to secondary consequences from the expression of a separate gene, nor to a particular property of the BCE-1 construct. As a component of this investigation, we also showed that ECM could mediate rapid histone deacetylation in mammary epithelial cells. These results are discussed in combination with previous work showing that TSA mediates the differentiation of many types of cancer cells but inhibits differentiation of some nonmalignant cell types.

Pujuguet, Philippe; Radisky, Derek; Levy, Dinah; Lacza, Charlemagne; Bissell, Mina J.

2002-02-22T23:59:59.000Z

4

Matrix Metalloproteinase Stromelysin-1 Triggers a Cascade of Molecular Alterations that leads to stable epithelial-to-Mesenchymal Conversion and a Premalignant Phenotype in Mammary Epithelial Cells  

SciTech Connect (OSTI)

Matrix metalloproteinases (MMPs) regulate ductal morphogenesis, apoptosis, and neoplastic progression in mammary epithelial cells. To elucidate the direct effects of MMPs on mammary epithelium, we generated functionally normal cells expressing an inducible autoactivating stromelysin-1 (SL-1) transgene. Induction of SL-1 expression resulted in cleavage of E-cadherin, and triggered progressive phenotypic conversion characterized by disappearance of E-cadherin and catenins from cell-cell contacts, downregulation of cytokeratins, upregulation of vimentin, induction of keratinocyte growth factor expression and activation, and upregulation of endogenous MMPs. Cells expressing SL-1 were unable to undergo lactogenic differentiation and became invasive. Once initiated, this phenotypic conversion was essentially stable, and progressed even in the absence of continued SL-1 expression. These observations demonstrate that inappropriate expression of SL-1 initiates a cascade of events that may represent a coordinated program leading to loss of the differentiated epithelial phenotype and gain of some characteristics of tumor cells. Our data provide novel insights into how MMPs function in development and neoplastic conversion.

Lochter, A.; Galosy, S.; Muschler, J.; Freedman, N.; Werb, Z.; Bissell, M.J.

1997-08-11T23:59:59.000Z

5

Persistence of gamma-H2AX and 53BP1 foci in proliferating and nonproliferating human mammary epithelial cells after exposure to gamma-rays or iron ions  

SciTech Connect (OSTI)

To investigate {gamma}-H2AX (phosphorylated histone H2AX) and 53BP1 (tumour protein 53 binding protein No. 1) foci formation and removal in proliferating and non-proliferating human mammary epithelial cells (HMEC) after exposure to sparsely and densely ionizing radiation under different cell culture conditions. HMEC cells were grown either as monolayers (2D) or in extracellular matrix to allow the formation of acinar structures in vitro (3D). Foci numbers were quantified by image analysis at various time points after exposure. Our results reveal that in non-proliferating cells under 2D and 3D cell culture conditions, iron-ion induced {gamma}-H2AX foci were still present at 72 h after exposure, although 53BP1 foci returned to control levels at 48 h. In contrast in proliferating HMEC, both {gamma}-H2AX and 53BP1 foci decreased to control levels during the 24-48 h time interval after irradiation under 2D conditions. Foci numbers decreased faster after {gamma}-ray irradiation and returned to control levels by 12 h regardless of marker, cell proliferation status, and cell culture condition. Conclusions: The disappearance of radiation induced {gamma}-H2AX and 53BP1 foci in HMEC have different dynamics that depend on radiation quality and proliferation status. Notably, the general patterns do not depend on the cell culture condition (2D versus 3D). We speculate that the persistent {gamma}-H2AX foci in iron-ion irradiated non-proliferating cells could be due to limited availability of double strand break (DSB) repair pathways in G0/G1-phase, or that repair of complex DSB requires replication or chromatin remodeling.

Groesser, Torsten; Chang, Hang; Fontenay, Gerald; Chen, James; Costes, Sylvain V.; Barcellos-Hoff, Mary Helen; Parvin, Bahram; Rydberg, Bjorn

2010-12-22T23:59:59.000Z

6

EGF-receptor phosphorylation and downstream signaling are activated by benzo[a]pyrene 3,6-quinone and benzo[a]pyrene 1,6-quinone in human mammary epithelial cells  

SciTech Connect (OSTI)

Benzo[a]pyrene (BaP) is activated by xenobiotic-metabolizing enzymes to highly mutagenic and carcinogenic metabolites. Previous studies in this laboratory have shown that benzo[a]pyrene quinones (BPQs), 1,6-BPQ and 3,6-BPQ, are able to induce epidermal growth factor receptor (EGFR) cell signaling through the production of reactive oxygen species. Recently, we have reported that BPQs have the potential to induce the expression of genes involved in numerous pathways associated with cell proliferation and survival in human mammary epithelial cells. In the present study we demonstrated that BPQs not only induced EGFR tyrosine autophosphorylation, but also induced EGFR-dependent tyrosine phosphorylation of phospholipase C-{gamma}1 and several signal transducers and activators of transcription (STATs). The effects of BPQs were evaluated in a model of EGF withdrawal in MCF10-A cells. We found that BPQs (1 {mu}M), induced EGFR tyrosine phosphorylation at positions Y845, Y992, Y1068, and Y1086. PLC-{gamma}1 phosphorylation correlated with the phosphorylation of tyrosine-Y992, a proposed docking site for PLC-{gamma}1 on the EGFR. Additionally, we found that BPQs induced the activation of STAT-1, STAT-3, STAT-5a and STAT-5b. STAT5 was shown to translocate to the nucleus following 3,6-BPQ and 1,6-BPQ exposures. Although the patterns of phosphorylation at EGFR, PLC-{gamma}1 and STATs were quite similar to those induced by EGF, an important difference between BPQ-mediated signaling of the EGFR was observed. Signaling produced by EGF ligand produced a rapid disappearance of EGFR from the cell surface, whereas BPQ signaling maintained EGFR receptors on the cell membrane. Thus, the results of these studies show that 1,6-BPQ and 3,6-BPQ can produce early events as evidenced by EGFR expression, and a prolonged transactivation of EGFR leading to downstream cell signaling pathways.

Rodriguez-Fragoso, Lourdes [Facultad de Farmacia, Universidad Autonoma del Estado de Morelos, Avenida Universidad 1001 Col. Chamilpa, Cuernavaca 62210, Morelos (Mexico); Melendez, Karla; Hudson, Laurie G.; Lauer, Fredine T. [University of New Mexico, College of Pharmacy Toxicology Program, Albuquerque, New Mexico (United States); Burchiel, Scott W. [University of New Mexico, College of Pharmacy Toxicology Program, Albuquerque, New Mexico (United States)], E-mail: sburchiel@salud.unm.edu

2009-03-15T23:59:59.000Z

7

Of Microenvironments and Mammary Stem Cells  

SciTech Connect (OSTI)

In most adult tissues there reside pools of stem and progenitor cells inside specialized microenvironments referred to as niches. The niche protects the stem cells from inappropriate expansion and directs their critical functions. Thus guided, stem cells are able to maintain tissue homeostasis throughout the ebb and flow of metabolic and physical demands encountered over a lifetime. Indeed, a pool of stem cells maintains mammary gland structure throughout development, and responds to the physiological demands associated with pregnancy. This review discusses how stem cells were identified in both human and mouse mammary glands; each requiring different techniques that were determined by differing biological needs and ethical constraints. These studies together create a robust portrait of mammary gland biology and identify the location of the stem cell niche, elucidate a developmental hierarchy, and suggest how the niche might be manipulated for therapeutic benefit.

LaBarge, Mark A; Petersen, Ole W; Bissell, Mina J

2007-06-01T23:59:59.000Z

8

Stromal-epithelial interactions in aging and cancer: Senescent fibroblasts alter epithelial cell differentiation  

SciTech Connect (OSTI)

Cellular senescence suppresses cancer by arresting cells at risk for malignant tumorigenesis. However, senescent cells also secrete molecules that can stimulate premalignant cells to proliferate and form tumors, suggesting the senescence response is antagonistically pleiotropic. We show that premalignant mammary epithelial cells exposed to senescent human fibroblasts in mice irreversibly lose differentiated properties, become invasive and undergo full malignant transformation. Moreover, using cultured mouse or human fibroblasts and non-malignant breast epithelial cells, we show that senescent fibroblasts disrupt epithelial alveolar morphogenesis, functional differentiation, and branching morphogenesis. Further, we identify MMP-3 as the major factor responsible for the effects of senescent fibroblasts on branching morphogenesis. Our findings support the idea that senescent cells contribute to age-related pathology, including cancer, and describe a new property of senescent fibroblasts--the ability to alter epithelial differentiation--that might also explain the loss of tissue function and organization that is a hallmark of aging.

Parrinello, Simona; Coppe, Jean-Philippe; Krtolica, Ana; Campisi, Judith

2004-07-14T23:59:59.000Z

9

Regulation of Mammary Lactogenic Differentiation by Singleminded-2s  

E-Print Network [OSTI]

juvenile gland showing TEB structures. H. WM mature mammary gland. I, WM mammary gland at pregnancy day 10. J, WM mammary gland during lactation. 3 Studies performed in mice lacking ESR1, ESR2, Pgr, GHR, or PrlR revealed that embryonic... tumor necrosis factor (TNF) ligand superfamily member, and its receptor, EdaR, are expressed in the mesenchyme and epithelial placode cells, respectively (Pispa et al. 2003). Overexpression of Eda in mice results in supernumerary and enlarged mammary...

Wellberg, Elizabeth

2010-07-14T23:59:59.000Z

10

Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells  

E-Print Network [OSTI]

18 and 19 (K18–K19), vimentin (vim) and ?-sm actin (ASMA) inDako), and vimentin (VIM; MEDAC, GmbH). Other antibodiesa mAb against vimentin (VIM, IgG1). The secondary antibodies

Pechoux, Christine

2010-01-01T23:59:59.000Z

11

Pim-1 kinase expression during murine mammary development  

SciTech Connect (OSTI)

Pim-1 kinase phosphorylates substrates whose activities are linked to proliferation, survival, differentiation, and apoptosis. Although pim-1 is induced by hormones and cytokines, the hormonal control and contribution of Pim-1 to mammary gland development have not been evaluated. We examined Pim-1 expression in mammary cell lines, investigated whether Pim-1 levels could be altered in breast epithelia by mammogenic hormones, and evaluated Pim-1 expression during mammary development. We found that Pim-1 was elevated in most mammary carcinoma cell lines and progesterone increased Pim-1 protein to some extent in non-tumorigenic mammary epithelia. Pim-1 expression in situ was consistent with the documented profile of progesterone activity in mouse mammary glands. Pim-1 nuclear localization correlated with cytoplasmic distribution for its substrate, p21{sup CIP/Waf1}, and we found that Pim-1 and p21 associate in vitro. Our results suggest that Pim-1 expression may be regulated by progesterone during mammary development and Pim-1 associates with p21 in mammary epithelial cells.

Gapter, Leslie A. [Department of Pharmacy, Faculty of Science, National University of Singapore, 117543 (Singapore); School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4234 (United States); Magnuson, Nancy S. [School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4234 (United States); Ng, Ka-yun [Department of Pharmacy, Faculty of Science, National University of Singapore, 117543 (Singapore); Hosick, Howard L. [School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4234 (United States)]. E-mail: hosick@wsu.edu

2006-07-07T23:59:59.000Z

12

Probiotics promote endocytic allergen degradation in gut epithelial cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Knockdown of A20 compromised the epithelial barrier function. Black-Right-Pointing-Pointer The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Black-Right-Pointing-Pointer Antigens transported across A20-deficient HT-29 monolayers conserved antigenicity. Black-Right-Pointing-Pointer Probiotic proteins increased the expression of A20 in HT-29 cells. -- Abstract: Background and aims: Epithelial barrier dysfunction plays a critical role in the pathogenesis of allergic diseases; the mechanism is to be further understood. The ubiquitin E3 ligase A20 (A20) plays a role in the endocytic protein degradation in the cells. This study aims to elucidate the role of A20 in the maintenance of gut epithelial barrier function. Methods: Gut epithelial cell line, HT-29 cell, was cultured into monolayers to evaluate the barrier function in transwells. RNA interference was employed to knock down the A20 gene in HT-29 cells to test the role of A20 in the maintenance of epithelial barrier function. Probiotic derived proteins were extracted from the culture supernatants using to enhance the expression of A20 in HT-29 cells. Results: The results showed that the knockdown of A20 compromised the epithelial barrier function in HT-29 monolayers, mainly increased the intracellular permeability. The fusion of endosome/lysosome was disturbed in the A20-deficient HT-29 cells. Allergens collected from the transwell basal chambers of A20-deficient HT-29 monolayers still conserved functional antigenicity. Treating with probiotic derived proteins increased the expression of A20 in HT-29 cells and promote the barrier function. Conclusion: A20 plays an important role in the maintenance of epithelial barrier function as shown by HT-29 monolayer. Probiotic derived protein increases the expression of A20 and promote the HT-29 monolayer barrier function.

Song, Chun-Hua [Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou (China)] [Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou (China); Liu, Zhi-Qiang [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China) [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Huang, Shelly [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)] [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada); Zheng, Peng-Yuan, E-mail: medp7123@126.com [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China)] [Department of Gastroenterology, The Second Hospital, Zhengzhou University, Zhengzhou (China); Yang, Ping-Chang, E-mail: yangp@mcmaster.ca [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)] [Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON (Canada)

2012-09-14T23:59:59.000Z

13

Compressive stress enhances coordinated migration of mammary carcinoma cells  

E-Print Network [OSTI]

Cancer research has traditionally focused on genetic and biochemical changes during tumor progression. Uncontrolled cell proliferation of a solid tumor in a confined space not only creates well-studied oxidative stress ...

Tse, Janet M. (Janet Man-Yu)

2010-01-01T23:59:59.000Z

14

Bile Salts and Nuclear Receptors in Biliary Epithelial Cell Pathophysiology  

E-Print Network [OSTI]

Bile Salts and Nuclear Receptors in Biliary Epithelial Cell Pathophysiology by Dr. Nicolas Chignard shaped the way I perform my work today. Among many other examples, she showed me how to simply performed by students that I had the pleasure to supervise. I'm grateful to all of them. I especially would

Boyer, Edmond

15

Developmental Cell Three-Dimensional Epithelial Morphogenesis  

E-Print Network [OSTI]

@princeton.edu http://dx.doi.org/10.1016/j.devcel.2013.01.017 SUMMARY Morphogenesis of the respiratory appendages on eggshells of Drosophila species provides a powerful experimental system for studying how cell sheets give changes in morphology. Computational modeling shows that this mechanism could explain the main features

Shvartsman, Stanislav "Stas"

16

Laminin and biomimetic extracellular elasticity enhance functional differentiation in mammary epithelia  

SciTech Connect (OSTI)

In the mammary gland, epithelial cells are embedded in a 'soft' environment and become functionally differentiated in culture when exposed to a laminin-rich extracellular matrix gel. Here, we define the processes by which mammary epithelial cells integrate biochemical and mechanical extracellular cues to maintain their differentiated phenotype. We used single cells cultured on top of gels in conditions permissive for {beta}-casein expression using atomic force microscopy to measure the elasticity of the cells and their underlying substrata. We found that maintenance of {beta}-casein expression required both laminin signalling and a 'soft' extracellular matrix, as is the case in normal tissues in vivo, and biomimetic intracellular elasticity, as is the case in primary mammary epithelial organoids. Conversely, two hallmarks of breast cancer development, stiffening of the extracellular matrix and loss of laminin signalling, led to the loss of {beta}-casein expression and non-biomimetic intracellular elasticity. Our data indicate that tissue-specific gene expression is controlled by both the tissues unique biochemical milieu and mechanical properties, processes involved in maintenance of tissue integrity and protection against tumorigenesis.

Alcaraz, Jordi; Xu, Ren; Mori, Hidetoshi; Nelson, Celeste M.; Mroue, Rana; Spencer, Virginia A.; Brownfield, Doug; Radisky, Derek C.; Bustamante, Carlos; Bissell, Mina J.

2008-10-20T23:59:59.000Z

17

Effects of Carbon Nanotubes in Barrier Epithelial Cells via Effects on Lipid Bilayers Shanta Lewis1  

E-Print Network [OSTI]

Effects of Carbon Nanotubes in Barrier Epithelial Cells via Effects on Lipid Bilayers Shanta Lewis1 Physiology, IU School of Medicine Carbon nanotubes (CNTs) are one of many nanoparticles (NP) which are being

Zhou, Yaoqi

18

Epithelial–mesenchymal transition during oncogenic transformation induced by hexavalent chromium involves reactive oxygen species-dependent mechanism in lung epithelial cells  

SciTech Connect (OSTI)

Hexavalent chromium [Cr(VI)] is an important human carcinogen associated with pulmonary diseases and lung cancer. Exposure to Cr(VI) induces DNA damage, cell morphological change and malignant transformation in human lung epithelial cells. Despite extensive studies, the molecular mechanisms remain elusive, it is also not known if Cr(VI)-induced transformation might accompany with invasive properties to facilitate metastasis. We aimed to study Cr(VI)-induced epithelial–mesenchymal transition (EMT) and invasion during oncogenic transformation in lung epithelial cells. The results showed that Cr(VI) at low doses represses E-cadherin mRNA and protein expression, enhances mesenchymal marker vimentin expression and transforms the epithelial cell into fibroblastoid morphology. Cr(VI) also increases cell invasion and promotes colony formation. Further studies indicated that Cr(VI) uses multiple mechanisms to repress E-cadherin expression, including activation of E-cadherin repressors such as Slug, ZEB1, KLF8 and enhancement the binding of HDAC1 in E-cadherin gene promoter, but DNA methylation is not responsible for the loss of E-cadherin. Catalase reduces Cr(VI)-induced E-cadherin and vimentin protein expression, attenuates cell invasion in matrigel and colony formation on soft agar. These results demonstrate that exposure to a common human carcinogen, Cr(VI), induces EMT and invasion during oncogenic transformation in lung epithelial cells and implicate in cancer metastasis and prevention. - Graphical abstract: Epithelial–mesenchymal transition during oncogenic transformation induced by hexavalent chromium involves reactive oxygen species-dependent mechanisms in lung epithelial cells. - Highlights: • We study if Cr(VI) might induce EMT and invasion in epithelial cells. • Cr(VI) induces EMT by altering E-cadherin and vimentin expression. • It also increases cell invasion and promotes oncogenic transformation. • Catalase reduces Cr(VI)-induced EMT, invasion and transformation.

Ding, Song-Ze, E-mail: dingsongze@hotmail.com [Department of Internal Medicine, Henan Provincial People’s Hospital, Zhengzhou University, Wei-Wu Road, Zhengzhou, Henan 450000 (China); Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536 (United States); Yang, Yu-Xiu; Li, Xiu-Ling [Department of Internal Medicine, Henan Provincial People’s Hospital, Zhengzhou University, Wei-Wu Road, Zhengzhou, Henan 450000 (China); Michelli-Rivera, Audrey [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536 (United States); Han, Shuang-Yin [Department of Internal Medicine, Henan Provincial People’s Hospital, Zhengzhou University, Wei-Wu Road, Zhengzhou, Henan 450000 (China); Wang, Lei; Pratheeshkumar, Poyil; Wang, Xin; Lu, Jian; Yin, Yuan-Qin; Budhraja, Amit; Hitron, Andrew J. [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY 40536 (United States)

2013-05-15T23:59:59.000Z

19

The plasticity of human breast carcinoma cells is more than epithelial to mesenchymal conversion  

SciTech Connect (OSTI)

The human breast comprises three lineages: the luminal epithelial lineage, the myoepithelial lineage, and the mesenchymal lineage. It has been widely accepted that human breast neoplasia pertains only to the luminal epithelial lineage. In recent years, however, evidence has accumulated that neoplastic breast epithelial cells may be substantially more plastic in their differentiation repertoire than previously anticipated. Thus, along with an increasing availability of markers for the myoepithelial lineage, at least a partial differentiation towards this lineage is being revealed frequently. It has also become clear that conversions towards the mesenchymal lineage actually occur, referred to as epithelial to mesenchymal transitions. Indeed, some of the so-called myofibroblasts surrounding the tumor may indeed have an epithelial origin rather than a mesenchymal origin. Because myoepithelial cells, epithelial to mesenchymal transition-derived cells, genuine stromal cells and myofibroblasts share common markers, we now need to define a more ambitious set of markers to distinguish these cell types in the microenvironment of the tumors. This is necessary because the different microenvironments may confer different clinical outcomes. The aim of this commentary is to describe some of the inherent complexities in defining cellular phenotypes in the microenvironment of breast cancer and to expand wherever possible on the implications for tumor suppression and progression.

Petersen, Ole William; Nielsen, Helga Lind; Gudjonsson, Thorarinn; Villadsen, Ren& #233; Ronnov-Jessen, Lone; Bissell, Mina J.

2001-05-12T23:59:59.000Z

20

Stromal-epithelial interactions in aging and cancer: Senescent fibroblasts alter epithelial cell differentiation  

E-Print Network [OSTI]

Cell. Biol. 18, 4577-4588. DiLeonardo, A. , Linke, S. P. ,Chen et al. , 1995; DiLeonardo et al. , 1994; Krtolica andsenesce by X-irradiation (DiLeonardo et al. , 1994; Robles

Parrinello, Simona; Coppe, Jean-Philippe; Krtolica, Ana; Campisi, Judith

2004-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Expression of Phospholipases A2 and C in Human Corneal Epithelial Cells  

E-Print Network [OSTI]

-healing pro- cesses in human corneal epithelial cells (HCECs), expression of phospholipase A2s (PLA2s for RT-PCR amplifi- cation of the known secreted (s)PLA2, cytosolic (c)PLA2, and PLC mRNAs. Corresponding and Western blot analyses were used to detect the PLA2s and PLCs expressed by HCECs. RESULTS. The m

Gelb, Michael

22

Modeling gas phase nitric oxide release in lung epithelial cells Jingjing Jiang a  

E-Print Network [OSTI]

Modeling gas phase nitric oxide release in lung epithelial cells Jingjing Jiang a , Steven C- dated our model with experimental results of gas phase NO release and intracellular L enzyme on NO production. Our model predicts intracellular L-arginine and gas phase NO release over a wide

George, Steven C.

23

In vitro models for airway epithelial cell culture  

E-Print Network [OSTI]

This work is about the development of a physiologically relevant model of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear ...

Sivathanu, Vivek

2013-01-01T23:59:59.000Z

24

Mechanisms of lead transport in two intestinal epithelial cell lines  

E-Print Network [OSTI]

. . . . . . . . . . . . . . . . . . . . . . . . Effects of intracellular binding proteins on Pb absorption. . . . Factors affecting intestinal Pb absorption. Pb absorption by other cell types Potential components of Pb absorption pathways in enterocytes. MATERIALS AND METHODS 4 5 7 10 12 14... of sulfhydryl modifiers on total cellular Pb content in IEC-6 cells. ????. ?, . ?. .?, . 32 10 Effects of RGD binding on the total cellular Pb content of IEC-6 cells. Effects of overnight exposure to Zn on the total cellular Pb content in Caco-2 monolayers...

Dekaney, Christopher Matthew

2012-06-07T23:59:59.000Z

25

alveolar epithelial type-1: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the role of oral immunity in this disease. Outi Vaarala 34 Epithelial-Specific and Stage-Specific Functions of Insulin-Like Growth Factor-I during Postnatal Mammary...

26

Phosphatidylinositol 4-Phosphate 5-Kinase Reduces Cell Surface Expression of the Epithelial Sodium Channel (ENaC)  

E-Print Network [OSTI]

Phosphatidylinositol 4-Phosphate 5-Kinase Reduces Cell Surface Expression of the Epithelial Sodium levels and reduced apical surface expression of ENaC in CCD cells, down-regulating amiloride- sensitive Channel (ENaC) in Cultured Collecting Duct Cells* Received for publication,May 14, 2007, and in revised

Weisz, Ora A.

27

A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland  

E-Print Network [OSTI]

% glutaraldehyde in 0.1 mol/l sodium cacodylate/HCl buffer of pH 7.2–7.4 at 4°C for 48 h. After washing with distilled water (dH2O) for 20 min, the samples underwent secondary fixation in 1% osmium tetroxide in dH2O for 45 min at room temperature. Samples were... .0 0 4 × 103 9.2 ± 1.2 0 2 × 103 8.4 ± 2.0 0 1 × 103 6.4 ± 1.7 0 Cells were seeded at five different densities in soft agar and grown for 14 days. Colonies were defined as spherical growth of cells of 5 mm in diameter or more. Values shown are the means...

Gordon, Katrina E; Binas, Bert; Chapman, Rachel S; Kurian, Kathreena M; Clarkson, Richard W E; Clark, A John; Birgitte Lane, E; Watson, Christine J

2000-03-07T23:59:59.000Z

28

Uptake and cytotoxic effects of multi-walled carbon nanotubes in human bronchial epithelial cells  

SciTech Connect (OSTI)

Carbon nanotubes (CNT) are cytotoxic to several cell types. However, the mechanism of CNT toxicity has not been fully studied, and dosimetric analyses of CNT in the cell culture system are lacking. Here, we describe a novel, high throughput method to measure cellular uptake of CNT using turbimetry. BEAS-2B, a human bronchial epithelial cell line, was used to investigate cellular uptake, cytotoxicity, and inflammatory effects of multi-walled CNT (MWCNT). The cytotoxicity of MWCNT was higher than that of crocidolite asbestos in BEAS-2B cells. The IC{sub 50} of MWCNT was 12 {mu}g/ml, whereas that of asbestos (crocidolite) was 678 {mu}g/ml. Over the course of 5 to 8 h, BEAS-2B cells took up 17-18% of the MWCNT when they were added to the culture medium at a concentration of 10 {mu}g/ml. BEAS-2B cells were exposed to 2, 5, or 10 {mu}g/ml of MWCNT, and total RNA was extracted for cytokine cDNA primer array assays. The culture supernatant was collected for cytokine antibody array assays. Cytokines IL-6 and IL-8 increased in a dose dependent manner at both the mRNA and protein levels. Migration inhibitory factor (MIF) also increased in the culture supernatant in response to MWCNT. A phosphokinase array study using lysates from BEAS-2B cells exposed to MWCNT indicated that phosphorylation of p38, ERK1, and HSP27 increased significantly in response to MWCNT. Results from a reporter gene assays using the NF-{kappa}B or AP-1 promoter linked to the luciferase gene in transiently transfected CHO-KI cells revealed that NF-{kappa}B was activated following MWCNT exposure, while AP-1 was not changed. Collectively, MWCNT activated NF-{kappa}B, enhanced phosphorylation of MAP kinase pathway components, and increased production of proinflammatory cytokines in human bronchial epithelial cells.

Hirano, Seishiro, E-mail: seishiro@nies.go.j [Environmental Nanotoxicology Section, RCER, National Institute for Environmental Studies (Japan); Fujitani, Yuji; Furuyama, Akiko [Environmental Nanotoxicology Section, RCER, National Institute for Environmental Studies (Japan); Kanno, Sanae [Photon Medical Research Center, Hamamatsu University School of Medicine (Japan)

2010-11-15T23:59:59.000Z

29

Chlorobenzene induces oxidative stress in human lung epithelial cells in vitro  

SciTech Connect (OSTI)

Chlorobenzene is a volatile organic compound (VOC) that is widely used as a solvent, degreasing agent and chemical intermediate in many industrial settings. Occupational studies have shown that acute and chronic exposure to chlorobenzene can cause irritation of the mucosa of the upper respiratory tract and eyes. Using in vitro assays, we have shown in a previous study that human bronchial epithelial cells release inflammatory mediators such as the cytokine monocyte chemoattractant protein-1 (MCP-1) in response to chlorobenzene. This response is mediated through the NF-kappaB signaling pathway. Here, we investigated the effects of monochlorobenzene on human lung cells, with emphasis on potential alterations of the redox equilibrium to clarify whether the chlorobenzene-induced inflammatory response in lung epithelial cells is caused via an oxidative stress-dependent mechanism. We found that expression of cellular markers for oxidative stress, such as heme oxygenase 1 (HO-1), glutathione S-transferase pi1 (GSTP1), superoxide dismutase 1 (SOD1), prostaglandin-endoperoxide synthase 2 (PTGS2) and dual specificity phosphatase 1 (DUSP1), were elevated in the presence of monochlorobenzene. Likewise, intracellular reactive oxygen species (ROS) were increased in response to exposure. However, in the presence of the antioxidants N-(2-mercaptopropionyl)-glycine (MPG) or bucillamine, chlorobenzene-induced upregulation of marker proteins and release of the inflammatory mediator MCP-1 are suppressed. These results complement our previous findings and point to an oxidative stress-mediated inflammatory response following chlorobenzene exposure.

Feltens, Ralph, E-mail: ralph.feltens@ufz.d [UFZ- Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany); UFZ- Helmholtz Centre for Environmental Research, Department of Proteomics, Permoserstrasse 15, D-04318 Leipzig (Germany); Moegel, Iljana, E-mail: iljana.moegel@ufz.d [UFZ- Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany); Roeder-Stolinski, Carmen, E-mail: carmen.roeder-stolinski@ufz.d [UFZ- Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany); Simon, Jan-Christoph, E-mail: Jan-Christoph.Simon@medizin.uni-leipzig.d [Leipzig University Medical Center, Clinic of Dermatology, Venerology and Allergology, Philipp-Rosenthal-Str. 23-25, D-4103 Leipzig (Germany); Herberth, Gunda, E-mail: gunda.herberth@ufz.d [UFZ- Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany); Lehmann, Irina, E-mail: irina.lehmann@ufz.d [UFZ- Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig (Germany)

2010-01-01T23:59:59.000Z

30

Intestinal-fatty acid binding protein and lipid transport in human intestinal epithelial cells  

SciTech Connect (OSTI)

Intestinal-fatty acid binding protein (I-FABP) is a 14-15 kDa cytoplasmic molecule highly expressed in the enterocyte. Although different functions have been proposed for various FABP family members, the specific function of I-FABP in human intestine remains unclear. Here, we studied the role of I-FABP in molecularly modified normal human intestinal epithelial cells (HIEC-6). cDNA transfection resulted in 90-fold I-FABP overexpression compared to cells treated with empty pQCXIP vector. The high-resolution immunogold technique revealed labeling mainly in the cytosol and confirmed the marked phenotype abundance of I-FABP in cDNA transfected cells. I-FABP overexpression was not associated with alterations in cell proliferation and viability. Studies using these transfected cells cultured with [{sup 14}C]oleic acid did not reveal higher efficiency in de novo synthesis or secretion of triglycerides, phospholipids, and cholesteryl esters compared to cells treated with empty pQCXIP vector only. Similarly, the incubation with [{sup 35}S]methionine did not disclose a superiority in the biogenesis of apolipoproteins (apo) A-I, A-IV, B-48, and B-100. Finally, cells transfected with I-FABP did not exhibit an increased production of chylomicrons, VLDL, LDL, and HDL. Our observations establish that I-FABP overexpression in normal HIEC-6 is not related to cell proliferation, lipid esterification, apo synthesis, and lipoprotein assembly, and, therefore, exclude its role in intestinal fat transport.

Montoudis, Alain [Department of Nutrition, Universite de Montreal and Research Center, CHU Sainte Justine, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada); Delvin, Edgard [Department of Biochemistry, Universite de Montreal and Research Center, CHU Sainte Justine, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada); Canadian Institute of Health Research, Group of the Functional Development and Physiopathology of the Digestive Tract, and Department of Anatomy and Cellular Biology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Que., Canada J1H 5N4 (Canada); Menard, Daniel [Department of Pathology and Cell Biology, Universite de Montreal and Research Center, CHU Sainte Justine, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada); Canadian Institute of Health Research, Group of the Functional Development and Physiopathology of the Digestive Tract, and Department of Anatomy and Cellular Biology, Faculty of Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Que., J1H 5N4 (Canada)] (and others)

2006-01-06T23:59:59.000Z

31

Autophagy protects type II alveolar epithelial cells from Mycobacterium tuberculosis infection  

SciTech Connect (OSTI)

Highlights: ? We investigated the protective effect of autophagy pathway against MTB infection. ? MTB-infected A549 cells had higher LDH release. ? Inhibition of autophagy signaling significantly enhanced the MTB-induced necrosis. ? Autophagy prevents apoptosis and promotes cell survival in infected cells. -- Abstract: This study was designed to investigate the protective effect of the autophagy signaling pathway against Mycobacterium tuberculosis infection in type II alveolar epithelial cells. An in vitro M. tuberculosis system was established using human A549 cells. Infection-induced changes in the expression of the autophagic marker LC3 were assessed by reverse transcription-PCR and Western blotting. Morphological changes in autophagosomes were detected by transmission electron microscopy (TEM). The function of the autophagy signaling pathway during infection was assessed by measuring the level of cell death and the amount of lactate dehydrogenase (LDH) released in the presence or absence of the inhibitor 3-methyladenine (3-MA). In addition, effects on LDH release were assessed after the siRNA-mediated knockdown of the essential autophagosomal structural membrane protein Atg5. LC3 mRNA expression was significantly reduced in M.tuberculosis-infected A549 cells (16888.76 ± 1576.34 vs. uninfected: 12744.29 ± 1089.37; P < 0.05). TEM revealed M.tuberculosis bacilli-containing compartments that were surrounded by double membranes characteristic of the autophagic process. M.tuberculosis-infected A549 cells released more LDH (1.45 ± 0.12 vs. uninfected: 0.45 ± 0.04; P < 0.05). The inhibition of autophagy signaling significantly enhanced M.tuberculosis-induced necrosis (3-MA: 75 ± 5% vs. untreated: 15 ± 1%; P < 0.05) and LDH release (3-MA: 2.50 ± 0.24 vs. untreated: 0.45 ± 0.04; Atg5 knockdown: 3.19 ± 0.29 vs. untreated: 1.28 ± 0.11; P < 0.05). Our results indicate that autophagy signaling pathway prevents apoptosis in type II alveolar epithelial cells infected with M.tuberculosis and may represent a molecular target for promoting cell survival during infection by respiratory pathogens.

Guo, Xu-Guang [Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an (China) [Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an (China); Department of Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou (China); Ji, Tian-Xing [Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou (China)] [Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou (China); Xia, Yong, E-mail: gysyxy@gmail.com [Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an (China)] [Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an (China); Ma, Yue-Yun, E-mail: cmbmayy@fmmu.edu.cn [Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an (China)] [Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an (China)

2013-03-08T23:59:59.000Z

32

3624 Biophysical Journal Volume 84 June 2003 36243635 Discrete Models of Autocrine Cell Communication in Epithelial Layers  

E-Print Network [OSTI]

of Chemical Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton-to- cell communication produces spatially nonuniform patterns in the expression of genes that guide the development of tissues and organs. The design principles of epithelial patterning are being formulated only

Shvartsman, Stanislav "Stas"

33

IL1{beta}-mediated Stromal COX-2 signaling mediates proliferation and invasiveness of colonic epithelial cancer cells  

SciTech Connect (OSTI)

COX-2 is a major inflammatory mediator implicated in colorectal inflammation and cancer. However, the exact origin and role of COX-2 on colorectal inflammation and carcinogenesis are still not well defined. Recently, we reported that COX-2 and iNOS signalings interact in colonic CCD18Co fibroblasts. In this article, we investigated whether activation of COX-2 signaling by IL1{beta} in primary colonic fibroblasts obtained from normal and cancer patients play a critical role in regulation of proliferation and invasiveness of human colonic epithelial cancer cells. Our results demonstrated that COX-2 level was significantly higher in cancer associated fibroblasts than that in normal fibroblasts with or without stimulation of IL-1{beta}, a powerful stimulator of COX-2. Using in vitro assays for estimating proliferative and invasive potential, we discovered that the proliferation and invasiveness of the epithelial cancer cells were much greater when the cells were co-cultured with cancer associated fibroblasts than with normal fibroblasts, with or without stimulation of IL1{beta}. Further analysis indicated that the major COX-2 product, prostaglandin E{sub 2}, directly enhanced proliferation and invasiveness of the epithelial cancer cells in the absence of fibroblasts. Moreover, a selective COX-2 inhibitor, NS-398, blocked the proliferative and invasive effect of both normal and cancer associate fibroblasts on the epithelial cancer cells, with or without stimulation of IL-1{beta}. Those results indicate that activation of COX-2 signaling in the fibroblasts plays a major role in promoting proliferation and invasiveness of the epithelial cancer cells. In this process, PKC is involved in the activation of COX-2 signaling induced by IL-1{beta} in the fibroblasts.

Zhu, Yingting, E-mail: yitizhu@yahoo.com [University of Arizona Arizona Cancer Center Tissue Tech Inc, 7000 SW 97th Avenue Suite 212, Miami, FL 33173 (United States) [University of Arizona Arizona Cancer Center Tissue Tech Inc, 7000 SW 97th Avenue Suite 212, Miami, FL 33173 (United States); Tissue Tech Inc, Miami, FL 33173 (United States); Zhu, Min; Lance, Peter [University of Arizona Arizona Cancer Center Tissue Tech Inc, 7000 SW 97th Avenue Suite 212, Miami, FL 33173 (United States)] [University of Arizona Arizona Cancer Center Tissue Tech Inc, 7000 SW 97th Avenue Suite 212, Miami, FL 33173 (United States)

2012-11-15T23:59:59.000Z

34

Biomolecular interactions and responses of human epithelial and macrophage cells to engineered nanomaterials.  

SciTech Connect (OSTI)

Engineered nanomaterials (ENMs) are increasingly being used in commercial products, particularly in the biomedical, cosmetic, and clothing industries. For example, pants and shirts are routinely manufactured with silver nanoparticles to render them 'wrinkle-free.' Despite the growing applications, the associated environmental health and safety (EHS) impacts are completely unknown. The significance of this problem became pervasive within the general public when Prince Charles authored an article in 2004 warning of the potential social, ethical, health, and environmental issues connected to nanotechnology. The EHS concerns, however, continued to receive relatively little consideration from federal agencies as compared with large investments in basic nanoscience R&D. The mounting literature regarding the toxicology of ENMs (e.g., the ability of inhaled nanoparticles to cross the blood-brain barrier; Kwon et al., 2008, J. Occup. Health 50, 1) has spurred a recent realization within the NNI and other federal agencies that the EHS impacts related to nanotechnology must be addressed now. In our study we proposed to address critical aspects of this problem by developing primary correlations between nanoparticle properties and their effects on cell health and toxicity. A critical challenge embodied within this problem arises from the ability to synthesize nanoparticles with a wide array of physical properties (e.g., size, shape, composition, surface chemistry, etc.), which in turn creates an immense, multidimensional problem in assessing toxicological effects. In this work we first investigated varying sizes of quantum dots (Qdots) and their ability to cross cell membranes based on their aspect ratio utilizing hyperspectral confocal fluorescence microscopy. We then studied toxicity of epithelial cell lines that were exposed to different sized gold and silver nanoparticles using advanced imaging techniques, biochemical analyses, and optical and mass spectrometry methods. Finally we evaluated a new assay to measure transglutaminase (TG) activity; a potential marker for cell toxicity.

Kotula, Paul Gabriel; Brozik, Susan Marie; Achyuthan, Komandoor E.; Greene, Adrienne Celeste; Timlin, Jerilyn Ann; Bachand, George David; Bachand, Marlene; Aaron, Jesse S.; Allen, Amy; Seagrave, Jean-Clare

2011-12-01T23:59:59.000Z

35

Differential transcriptional regulation of IL-8 expression by human airway epithelial cells exposed to diesel exhaust particles  

SciTech Connect (OSTI)

Exposure to diesel exhaust particles (DEP) induces inflammatory signaling characterized by MAP kinase-mediated activation of NFkB and AP-1 in vitro and in bronchial biopsies obtained from human subjects exposed to DEP. NFkB and AP-1 activation results in the upregulation of genes involved in promoting inflammation in airway epithelial cells, a principal target of inhaled DEP. IL-8 is a proinflammatory chemokine expressed by the airway epithelium in response to environmental pollutants. The mechanism by which DEP exposure induces IL-8 expression is not well understood. In the current study, we sought to determine whether DEP with varying organic content induces IL-8 expression in lung epithelial cells, as well as, to develop a method to rapidly evaluate the upstream mechanism(s) by which DEP induces IL-8 expression. Exposure to DEP with varying organic content differentially induced IL-8 expression and IL-8 promoter activity human airway epithelial cells. Mutational analysis of the IL-8 promoter was also performed using recombinant human cell lines expressing reporters linked to the mutated promoters. Treatment with a low organic-containing DEP stimulated IL-8 expression by a mechanism that is predominantly NFkB-dependent. In contrast, exposure to high organic-containing DEP induced IL-8 expression independently of NFkB through a mechanism that requires AP-1 activity. Our study reveals that exposure to DEP of varying organic content induces proinflammatory gene expression through multiple specific mechanisms in human airway epithelial cells. The approaches used in the present study demonstrate the utility of a promoter-reporter assay ensemble for identifying transcriptional pathways activated by pollutant exposure.

Tal, Tamara L. [Curriculum in Toxicology, University of North Carolina, Chapel Hill (United States); Simmons, Steven O. [Integrated Systems Toxicology, National Health and Environmental Effects Research Laboratory, U.S. EPA (United States); Silbajoris, Robert; Dailey, Lisa [Environmental and Public Health, National Health and Environmental Effects Research Laboratory, U.S. EPA (United States); Cho, Seung-Hyun [Air Pollution Prevention Control Division, National Risk Management Research Laboratory, U.S. EPA (United States); Research Participation Program, Oak Ridge Institute for Science and Education, Oak Ridge (United States); Ramabhadran, Ram [Curriculum in Toxicology, University of North Carolina, Chapel Hill (United States); Integrated Systems Toxicology, National Health and Environmental Effects Research Laboratory, U.S. EPA (United States); Linak, William [Air Pollution Prevention Control Division, National Risk Management Research Laboratory, U.S. EPA (United States); Reed, William; Bromberg, Philip A. [Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, Chapel Hill (United States); Samet, James M., E-mail: samet.james@epa.go [Curriculum in Toxicology, University of North Carolina, Chapel Hill (United States); Environmental and Public Health, National Health and Environmental Effects Research Laboratory, U.S. EPA (United States)

2010-02-15T23:59:59.000Z

36

E-Print Network 3.0 - alveolar epithelial type Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cells. Alveolar epithelial cells adherence to type I collagen was decreased... M CaCl2. Cell cultures Isolation and primary culture of alveolar epithelial cells Alveolar...

37

Stromal COX-2 signaling activated by deoxycholic acid mediates proliferation and invasiveness of colorectal epithelial cancer cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Human colonic cancer associated fibroblasts are major sources of COX-2 and PGE{sub 2}. Black-Right-Pointing-Pointer The fibroblasts interact with human colonic epithelial cancer cells. Black-Right-Pointing-Pointer Activation of COX-2 signaling in the fibroblasts affects behavior of the epithelia. Black-Right-Pointing-Pointer Protein Kinase C controls the activation of COX-2 signaling. -- Abstract: COX-2 is a major regulator implicated in colonic cancer. However, how COX-2 signaling affects colonic carcinogenesis at cellular level is not clear. In this article, we investigated whether activation of COX-2 signaling by deoxycholic acid (DCA) in primary human normal and cancer associated fibroblasts play a significant role in regulation of proliferation and invasiveness of colonic epithelial cancer cells. Our results demonstrated while COX-2 signaling can be activated by DCA in both normal and cancer associated fibroblasts, the level of activation of COX-2 signaling is significantly greater in cancer associated fibroblasts than that in normal fibroblasts. In addition, we discovered that the proliferative and invasive potential of colonic epithelial cancer cells were much greater when the cells were co-cultured with cancer associated fibroblasts pre-treated with DCA than with normal fibroblasts pre-treated with DCA. Moreover, COX-2 siRNA attenuated the proliferative and invasive effect of both normal and cancer associate fibroblasts pre-treated with DCA on the colonic cancer cells. Further studies indicated that the activation of COX-2 signaling by DCA is through protein kinase C signaling. We speculate that activation of COX-2 signaling especially in cancer associated fibroblasts promotes progression of colonic cancer.

Zhu, Yingting, E-mail: yitizhu@yahoo.com [Arizona Cancer Center, The University of Arizona, Tucson, AZ 85724 (United States) [Arizona Cancer Center, The University of Arizona, Tucson, AZ 85724 (United States); Tissue Tech Inc., Miami, FL 33173 (United States); Zhu, Min; Lance, Peter [Arizona Cancer Center, The University of Arizona, Tucson, AZ 85724 (United States)] [Arizona Cancer Center, The University of Arizona, Tucson, AZ 85724 (United States)

2012-08-31T23:59:59.000Z

38

Ionizing Radiation Promotes Migration and Invasion of Cancer Cells Through Transforming Growth Factor-Beta-Mediated Epithelial-Mesenchymal Transition  

SciTech Connect (OSTI)

Purpose: To examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-{beta})-mediated epithelial-mesenchymal transition (EMT). Methods and Materials: Six cancer cell lines originating from different human organs were irradiated by {sup 60}Co {gamma}-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-{beta} in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-{beta} signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. Results: After irradiation with {gamma}-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-{beta} were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-{beta} signaling. Conclusions: These results suggest that EMT mediated by TGF-{beta} plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

Zhou Yongchun [Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an (China); Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an (China); Liu Junye; Li Jing; Zhang Jie [Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an (China); Xu Yuqiao [Department of Pathology, Xijing Hospital Fourth Military Medical University, Xi'an (China); Zhang Huawei; Qiu Lianbo; Ding Guirong [Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an (China); Su Xiaoming [Department of Radiation Oncology, 306th Hospital of PLA, Beijing (China); Mei Shi [Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an (China); Guo Guozhen, E-mail: guozhenguo@hotmail.com [Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an (China)

2011-12-01T23:59:59.000Z

39

Sustained activation of STAT5 is essential for chromatin remodeling and maintenance of mammary-specific function  

SciTech Connect (OSTI)

Epithelial cells, once dissociated and placed in two-dimensional (2D) cultures, rapidly lose tissue-specific functions. We showed previously that in addition to prolactin, signaling by laminin-111 was necessary to restore functional differentiation of mammary epithelia. Here, we elucidate two additional aspects of laminin-111 action. We show that in 2D cultures, the prolactin receptor is basolaterally localized and physically segregated from its apically placed ligand. Detachment of the cells exposes the receptor to ligation by prolactin leading to signal transducers and activators of transcription protein 5 (STAT5) activation, but only transiently and not sufficiently for induction of milk protein expression. We show that laminin-111 reorganizes mammary cells into polarized acini, allowing both the exposure of the prolactin receptor and sustained activation of STAT5. The use of constitutively active STAT5 constructs showed that the latter is necessary and sufficient for chromatin reorganization and {beta}-casein transcription. These results underscore the crucial role of continuous laminin signaling and polarized tissue architecture in maintenance of transcription factor activation, chromatin organization, and tissue-specific gene expression.

Xu, Ren; Nelson, Celeste M.; Muschler, John L.; Veiseh, Mandana; Vonderhaar, Barbara K.; Bissell, Mina J.

2009-06-03T23:59:59.000Z

40

The transcription factor LEF-1 induces an epithelial–mesenchymal transition in MDCK cells independent of ?-catenin  

SciTech Connect (OSTI)

Highlights: •The transcription factor LEF-1 induces an EMT in MDCK cells. •A mutant LEF-1 that cannot interact with ?-catenin retained the ability. •The nuclear function of ?-catenin was not necessary for the LEF-1-induced EMT. •The mRNA levels of Slug, ZEB1, and ZEB2 increased significantly in these cells. -- Abstract: The epithelial–mesenchymal transition (EMT), a key process in the tumor metastatic cascade, is characterized by the loss of cell–cell junctions and cell polarity, as well as the acquisition of migratory and invasive properties. LEF-1 is a member of the lymphoid enhancer-binding factor/T-cell factor (LEF/TCF) family of DNA-binding transcription factors, which interact with nuclear ?-catenin and act as central transcriptional mediators of Wnt signaling. To investigate the role of LEF-1 in EMT, we generated stable LEF-1 transfectants using MDCK cells. The transfectants had a spindle-shaped mesenchymal morphology, and enhanced migration and invasiveness relative to control cells. These EMT changes were accompanied by the downregulation of an epithelial marker protein, E-cadherin, and the upregulation of mesenchymal marker proteins, vimentin and N-cadherin. Consistent with these observations, the mRNA levels of Slug, ZEB1, and ZEB2—EMT-related transcription factors—increased significantly. Although the N-terminally deleted mutant LEF-1 cannot interact with ?-catenin, it retained the ability to induce EMT. Consistent with these observations, neither the expression of a dominant negative ?-catenin/engrailed chimera, nor the expression of a cytoplasmic domain of E-cadherin that sequesters ?-catenin from binding to LEF/TCF, reversed LEF-1-induced EMT. Together, these data indicated that the nuclear function of ?-catenin was not necessary for the induction of Slug, ZEB1, and ZEB2 expression leading to EMT.

Kobayashi, Wakako; Ozawa, Masayuki, E-mail: mozawa@m.kufm.kagoshima-u.ac.jp

2013-12-06T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Decoupling Internalization, Acidification and Phagosmal-Endosomal/Iysosomal Phagocytosis of Internalin A coated Beads in epithelial cells  

SciTech Connect (OSTI)

Phagocytosis has been extensively examined in 'professional' phagocytic cells using pH sensitive dyes. However, in many of the previous studies, a separation between the end of internalization, beginning of acidification and completion of phagosomal-endosomal/lysosomal fusion was not clearly established, and in several cases, it was treated as a one-step process. In addition, very little work has been done to systematically examine phagosomal maturation in 'non-professional' phagocytic cells, such as epithelial cells. Therefore, in this study, we developed a simple and novel method to decouple and accurately measure particle internalization, phagosomal acidification and phagosomal-endosomal/lysosomal fusion in Madin-Darby Canine Kidney (MDCK) and Caco-2 epithelial cells. Our method was developed using a pathogen mimetic system consisting of polystyrene beads coated with Internalin A (InlA), a membrane surface protein from Listeria monocytogenes known to trigger receptor-mediated internalization. We achieved independent measurements of the rates of internalization, phagosomal acidification and phagosomal-endosomal/lysosomal fusion in epithelial cells by combining the InlA-coated beads (InlA-beads) with antibody quenching, pH sensitive dyes and endosomal/lysosomal dyes, as follows: the rate of InlA bead internalization was measured via antibody quenching of a pH independent dye (Alexa488) conjugated to InlA-beads, the rate at which phagosomes containing internalized InlA beads became acidified was measured using a pH dependent dye (FITC) conjugated to the beads and the rate of phagosomal-endosomal/lysosomal fusion was measured using a combination of unlabeled InlA-beads and an endosomal/lysosomal dye. By performing these independent measurements under identical experimental conditions, we were able to decouple the three processes and establish time scales for each. In a separate set of experiments, we also exploited the phagosomal acidification process to demonstrate an additional, real31 time method for tracking bead binding, internalization and phagosomal acidification in both MDCK and Caco-2 cells, as well as 1 NIH 3T3 fibroblast cells, using FITC conjugated to InlA-beads or fibronectin-coated beads. Using this method, we found that the time scales for internalization, phagosomal acidification and phagosomal-endosomal/lysosomal fusion were 23-32 min, 3-4 min and 74-120 min, respectively, for epithelial cells, MDCK and Caco-2, which are slower than the kinetics observed in professional phagocytes such as macrophages. Both the static and real-time methods developed here are expected to be readily and broadly applicable, as they simply require conjugation of a fluorophore to a pathogen or mimetic of interest in combination with common cell labeling dyes, and are not limited to the InlA ligand or cell types used here. As such, these methods hold promise for future measurements of receptor-mediated internalization in other cell systems, e.g. other pathogen-host systems.

Blanchette, C D; Woo, Y; Thomas, C; Shen, N; Sulchek, T A; Hiddessen, A L

2008-12-22T23:59:59.000Z

42

Chronic occupational exposure to arsenic induces carcinogenic gene signaling networks and neoplastic transformation in human lung epithelial cells  

SciTech Connect (OSTI)

Chronic arsenic exposure remains a human health risk; however a clear mode of action to understand gene signaling-driven arsenic carcinogenesis is currently lacking. This study chronically exposed human lung epithelial BEAS-2B cells to low-dose arsenic trioxide to elucidate cancer promoting gene signaling networks associated with arsenic-transformed (B-As) cells. Following a 6 month exposure, exposed cells were assessed for enhanced cell proliferation, colony formation, invasion ability and in vivo tumor formation compared to control cell lines. Collected mRNA was subjected to whole genome expression microarray profiling followed by in silico Ingenuity Pathway Analysis (IPA) to identify lung carcinogenesis modes of action. B-As cells displayed significant increases in proliferation, colony formation and invasion ability compared to BEAS-2B cells. B-As injections into nude mice resulted in development of primary and secondary metastatic tumors. Arsenic exposure resulted in widespread up-regulation of genes associated with mitochondrial metabolism and increased reactive oxygen species protection suggesting mitochondrial dysfunction. Carcinogenic initiation via reactive oxygen species and epigenetic mechanisms was further supported by altered DNA repair, histone, and ROS-sensitive signaling. NF-?B, MAPK and NCOR1 signaling disrupted PPAR?/?-mediated lipid homeostasis. A ‘pro-cancer’ gene signaling network identified increased survival, proliferation, inflammation, metabolism, anti-apoptosis and mobility signaling. IPA-ranked signaling networks identified altered p21, EF1?, Akt, MAPK, and NF-?B signaling networks promoting genetic disorder, altered cell cycle, cancer and changes in nucleic acid and energy metabolism. In conclusion, transformed B-As cells with their whole genome expression profile provide an in vitro arsenic model for future lung cancer signaling research and data for chronic arsenic exposure risk assessment. Highlights: ? Chronic As{sub 2}O{sub 3} exposure to lung epithelial cells resulted in a cancer-like phenotype. ? Mice injected with arsenic transformed (B-As) cells displayed metastatic tumors. ? Microarray profiling revealed changes in mitochondrial metabolism and ROS response. ? p21, EF1?, Akt, MAPK, PPAR? and NF-?B networks promoted pro-cancer signaling. ? B-As cells represent a lung cancer model to explore As-associated carcinogenesis.

Stueckle, Todd A., E-mail: tstueckle@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Lu, Yongju, E-mail: yongju6@hotmail.com [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Davis, Mary E., E-mail: mdavis@wvu.edu [Department of Physiology, West Virginia University, Morgantown, WV 26506 (United States); Wang, Liying, E-mail: lmw6@cdc.gov [Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States)] [Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Jiang, Bing-Hua, E-mail: bhjiang@jefferson.edu [Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)] [Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Holaskova, Ida, E-mail: iholaskova@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Schafer, Rosana, E-mail: rschafer@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Barnett, John B., E-mail: jbarnett@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Rojanasakul, Yon, E-mail: yrojan@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)

2012-06-01T23:59:59.000Z

43

Mangiferin exerts antitumor activity in breast cancer cells by regulating matrix metalloproteinases, epithelial to mesenchymal transition, and ?-catenin signaling pathway  

SciTech Connect (OSTI)

Although mangiferin which is a naturally occurring glucosylxanthone has exhibited promising anticancer activities, the detailed molecular mechanism of mangiferin on cancers still remains enigmatic. In this study, the anticancer activity of mangiferin was evaluated in breast cancer cell line-based in vitro and in vivo models. We showed that mangiferin treatment resulted in decreased cell viability and suppression of metastatic potential in breast cancer cells. Further mechanistic investigation revealed that mangiferin induced decreased matrix metalloproteinase (MMP)-7 and -9, and reversal of epithelial–mesenchymal transition (EMT). Moreover, it was demonstrated that mangiferin significantly inhibited the activation of ?-catenin pathway. Subsequent experiments showed that inhibiting ?-catenin pathway might play a central role in mangiferin-induced anticancer activity through modulation of MMP-7 and -9, and EMT. Consistent with these findings in vitro, the antitumor potential was also verified in mangiferin-treated MDA-MB-231 xenograft mice where significantly decreased tumor volume, weight and proliferation, and increased apoptosis were obtained, with lower expression of MMP-7 and -9, vimentin and active ?-catenin, and higher expression of E-cadherin. Taken together, our study suggests that mangiferin might be used as an effective chemopreventive agent against breast cancer. - Highlights: • Mangiferin inhibits growth and metastatic potential in breast cancer cells. • Mangiferin down-regulates MMP-7 and -9 in breast cancer cells. • Mangiferin induces the reversal of EMT in metastatic breast cancer cells. • Mangiferin inhibits the activation of ?-catenin pathway in breast cancer cells. • Inhibiting ?-catenin is responsible for the antitumor activity of mangiferin.

Li, Hongzhong; Huang, Jing; Yang, Bing; Xiang, Tingxiu; Yin, Xuedong; Peng, Weiyan; Cheng, Wei [Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing (China); Wan, Jingyuan; Luo, Fuling [Department of Pharmacology, Chongqing Medical University, Chongqing (China); Li, Hongyuan [Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing (China); Ren, Guosheng, E-mail: rgs726@163.com [Molecular Oncology and Epigenetics Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing (China)

2013-10-01T23:59:59.000Z

44

E-Print Network 3.0 - amniotic epithelial cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

in relatively low concen- trations in cell-free amniotic fluids from pregnancies with Tay-Sachs fetuses... amniotic fluid is used. Conceptually, either amniotic cells or...

45

E-Print Network 3.0 - anogenital epithelial cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cells Page: << < 1 2 3 4 5 > >> 1 Encoding Pheromonal Signals in the Accessory Olfactory Bulb of Summary: that a substantial number of cells responded in Fig. 5. Response of AOB...

46

miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohn’s disease  

SciTech Connect (OSTI)

Highlights: •NOD2 is a target gene of miR-122. •miR-122 inhibits LPS-induced apoptosis by suppressing NOD2 in HT-29 cells. •miR-122 reduces the expression of pro-inflammatory cytokines (TNF-? and IFN-?). •miR-122 promotes the release of anti-inflammatory cytokines (IL-4 and IL-10). •NF-?B signaling pathway is involved in inflammatory response induced by LPS. -- Abstract: Crohn’s disease (CD) is one of the two major types of inflammatory bowel disease (IBD) thought to be caused by genetic and environmental factors. Recently, miR-122 was found to be deregulated in association with CD progression. However, the underlying molecular mechanisms remain unclear. In the present study, the gene nucleotide-binding oligomerization domain 2 (NOD2/CARD15), which is strongly associated with susceptibility to CD, was identified as a functional target of miR-122. MiR-122 inhibited LPS-induced apoptosis by suppressing NOD2 in HT-29 cells. NOD2 interaction with LPS initiates signal transduction mechanisms resulting in the activation of nuclear factor ?B (NF-?B) and the stimulation of downstream pro-inflammatory events. The activation of NF-?B was inhibited in LPS-stimulated HT-29 cells pretreated with miR-122 precursor or NOD2 shRNA. The expression of the pro-inflammatory cytokines TNF-? and IFN-? was significantly decreased, whereas therelease of the anti-inflammatory cytokines IL-4 and IL-10 was increased in LPS-stimulated HT-29 cells pretreated with miR-122 precursor, NOD2 shRNA or the NF-?B inhibitor QNZ. Taken together, these results indicate that miR-122 and its target gene NOD2 may play an important role in the injury of intestinal epithelial cells induced by LPS.

Chen, Yu; Wang, Chengxiao; Liu, Ying; Tang, Liwei; Zheng, Mingxia [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China); Xu, Chundi [Department of Pediatrics, Ruijin affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200025 (China)] [Department of Pediatrics, Ruijin affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200025 (China); Song, Jian, E-mail: jiansongkxy@126.com [Department of Gastroenterology, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Gastroenterology, Jiangwan Hospital of Shanghai, Shanghai 200434 (China); Meng, Xiaochun [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)

2013-08-16T23:59:59.000Z

47

The metabolism of butyrate, glucose and glutamine in colonic epithelial cell lines  

E-Print Network [OSTI]

and glutamine are the major respiratory fuels for all three colonic cell lines. The rate of butyrate oxidation occurs in a dose dependent manner, while chronically inhibiting the rate of glutamine oxidation. Glycolysis is the major pathway accounting for glucose...

Shipley, Susan Grable

1997-01-01T23:59:59.000Z

48

Effects of prolactin on lipid biosynthesis and protein kinase C in mouse mammary gland and NB sub 2 node lymphoma cells  

SciTech Connect (OSTI)

In cultured mouse mammary gland explants derived from 12-14 day pregnant mice, prolactin (PRL) stimulates an increased rate of incorporation of ({sup 14}C)acetate and ({sup 3}H)glucose into triglycerides. The effect is significant between 4-6 hours after addition of PRL. Enzymes likely to be rate-limiting to this process include acetyl CoA carboxylase (ACC), fatty acid synthetase, acetyl CoA synthetase, and/or pyruvate dehydrogenase (PDH). It is possible that early perturbations of phospholipid (PL) metabolism may represent the initial cellular effects of PRL. Consequently the effect of PRL on the incorporation of several precursors into PLs was determined. Employing ({sup 14}C)acetate as a substrate, PRL stimulates its incorporation into phosphatidylcholine, as early as 1-2 hours, and phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine by 2-4 hours. ({sup 3}H)Glycerol incorporation into triglycerides was significantly enhanced by PRL between 4-6 hours, but not into PLs until after 16 hours. Similarly, PRL did not enhance incorporation of ({sup 32}P)O{sub 4}, ({sup 3}H)choline, ({sup 3}H)inositol or ({sup 3}H)seine into PLs until 14-16 hours after addition to culture. 12-O-tetradeconyl-phorbol-13-acetate (TPA) was found to increase ({sup 3}H)uridine incorporation into RNA, and ({sup 3}H)leucine incorporation into caseins in a PRL-like manner. In addition, PRL stimulates a transient, time-dependent translocation of PKC to the particulate fraction of mammary gland explants.

Waters, S.B.

1989-01-01T23:59:59.000Z

49

Aberrant, ectopic expression of VEGF and VEGF receptors 1 and 2 in malignant colonic epithelial cells. Implications for these cells growth via an autocrine mechanism  

SciTech Connect (OSTI)

Highlights: •Malignant colonic epithelial cells express VEGF and its receptors. •Cultured colon cancer cells secrete VEGF into the medium. •Inhibition of VEGF receptor significantly decreases colon cancer cell proliferation. •VEGF is critical for colon cancer cell growth. -- Abstract: Vascular endothelial growth factor A (referred to as VEGF) is implicated in colon cancer growth. Currently, the main accepted mechanism by which VEGF promotes colon cancer growth is via the stimulation of angiogenesis, which was originally postulated by late Judah Folkman. However, the cellular source of VEGF in colon cancer tissue; and, the expression of VEGF and its receptors VEGF-R1 and VEGF-R2 in colon cancer cells are not fully known and are subjects of controversy. Material and methods: We examined and quantified expression of VEGF, VEGF-R1 and VEGF-R2 in three different human colonic tissue arrays containing sections of adenocarcinoma (n = 43) and normal mucosa (n = 41). In human colon cancer cell lines HCT116 and HT29 and normal colon cell lines NCM356 and NCM460, we examined expression of VEGF, VEGF-R1 and VEGF-R2 mRNA and protein, VEGF production and secretion into the culture medium; and, the effect of a potent, selective inhibitor of VEGF receptors, AL-993, on cell proliferation. Results: Human colorectal cancer specimens had strong expression of VEGF in cancer cells and also expressed VEGF-R1 and VEGF-R2.In vitro studies showed that human colon cancer cell lines, HCT116 and HT29, but not normal colonic cell lines, express VEGF, VEGF-R1 and VEGF-R2 and secrete VEGF into the medium up to a concentration 2000 pg/ml within 48 h. Furthermore, we showed that inhibition of VEGF receptors using a specific VEGF-R inhibitor significantly reduced proliferation (by >50%) of cultured colon cancer cell lines. Conclusions: Our findings support the contention that VEGF generated by colon cancer cells stimulates their growth directly through an autocrine mechanism that is independent of its primary function in the induction of angiogenesis.

Ahluwalia, Amrita [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States)] [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States); Jones, Michael K. [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States) [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States); Department of Medicine, University of California, Irvine, CA (United States); Szabo, Sandor [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States) [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States); Department of Pathology, University of California, Irvine, CA (United States); Tarnawski, Andrzej S., E-mail: amrita.ahluwalia@va.gov [Veterans Affairs Long Beach Healthcare System, Long Beach, CA (United States); Department of Medicine, University of California, Irvine, CA (United States)

2013-08-09T23:59:59.000Z

50

Post Treatment With an FGF Chimeric Growth Factor Enhances Epithelial Cell Proliferation to Improve Recovery From Radiation-Induced Intestinal Damage  

SciTech Connect (OSTI)

Purpose: A fibroblast growth factor (FGF) 1-FGF2 chimera (FGFC) was created previously and showed greater structural stability than FGF1. This chimera was capable of stimulating epithelial cell proliferation much more strongly than FGF1 or FGF2 even without heparin. Therefore FGFC was expected to have greater biologic activity in vivo. This study evaluated and compared the protective activity of FGFC and FGF1 against radiation-induced intestinal injuries. Methods and Materials: We administered FGFC and FGF1 intraperitoneally to BALB/c mice 24 h before or after total-body irradiation (TBI). The numbers of surviving crypts were determined 3.5 days after TBI with gamma rays at doses ranging from 8 to 12 Gy. Results: The effect of FGFC was equal to or slightly superior to FGF1 with heparin. However, FGFC was significantly more effective in promoting crypt survival than FGF1 (p < 0.01) when 10 {mu}g of each FGF was administered without heparin before irradiation. In addition, FGFC was significantly more effective at promoting crypt survival (p < 0.05) than FGF1 even when administered without heparin at 24 h after TBI at 10, 11, or 12 Gy. We found that FGFC post treatment significantly promoted 5-bromo-2'-deoxyuridine incorporation into crypts and increased crypt depth, resulting in more epithelial differentiation. However, the number of apoptotic cells in FGFC-treated mice decreased to almost the same level as that in FGF1-treated mice. Conclusions: These findings suggest that FGFC strongly enhanced radioprotection with the induction of epithelial proliferation without exogenous heparin after irradiation and is useful in clinical applications for both the prevention and post treatment of radiation injuries.

Nakayama, Fumiaki, E-mail: f_naka@nirs.go.j [Department of Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba (Japan); Hagiwara, Akiko; Umeda, Sachiko [Department of Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba (Japan); Asada, Masahiro; Goto, Megumi; Oki, Junko; Suzuki, Masashi; Imamura, Toru [Signaling Molecules Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba (Japan); Akashi, Makoto [Department of Radiation Emergency Medicine, National Institute of Radiological Sciences, Chiba (Japan)

2010-11-01T23:59:59.000Z

51

The effect of DDT and its metabolite (DDE) on prostaglandin secretion from epithelial cells and on contractions of the smooth muscle of the bovine oviduct in vitro  

SciTech Connect (OSTI)

The insecticide DDT and its metabolite (DDE), due to their lipolytic nature and resistance to biodegradation, are accumulated in the living tissues. In cows, DDT and DDE were found to affect prostaglandin (PG) secretion from the endometrium and contractions of the myometrium. In this study, the impact of both xenobiotics (0.1, 1, 10 or 100 ng/ml) on the function of epithelial cells and muscle strips of bovine oviducts from 1 to 5 day of the oestrous cycle was examined. Therefore the concentration of PGE2 and PGFM (a metabolite of PGF2?) in culture media, mRNA expression of genes involved in PGs synthesis in epithelial cells and the force and amplitude of strips contractions were measured after 2 and 24 or 48 h of incubation. Neither DDT nor DDE affected the viability of cells after 48 h (P > 0.05). Both DDT and DDE increased the concentrations of PGFM in culture medium and secretion of PGE2 after only 2 h of cell culture (P < 0.05). Similar effects were seen for the influence of DDE on amount of PGFM after 48 h, while DDT decreased secretion of PGE2 (P < 0.05). DDT after 2 h increased (P < 0.05) mRNA expression of PGF2? synthase (PGFS), while both xenobiotics decreased (P < 0.05) mRNA expression of cyclooxygenase-2 (COX-2) after 24 h. DTT also increased the force of isthmus contractions after 2 h, as did both xenobiotics after 48 h (P < 0.05). Moreover, after 2 and 48 h, DDE stimulated the amplitude of contractions of the isthmus as well as the ampulla, (P < 0.05). The effect of both compounds on oviduct contractions was diminished by indomethacin, which blocks PG synthesis. We conclude that oviductal secretion of prostaglandins is affected, by DDT and DDE. The influence of these xenobiotics on PGF2? and PGE2 secretion and ratio may be part of the mechanism by which both DDT and its metabolite disturb the contractions of oviductal muscle. -- Highlights: ? DDT and its metabolite – DDE are accumulated in the living tissues. ? The insecticides affected PGF2? and PGE2 release from epithelial cells of oviduct. ? They also stimulated markedly the contractions of oviductal strips. ? Prostaglandins were involved in the effect of insecticides on oviduct function.

Wrobel, Michal H.; Mlynarczuk, Jaroslaw; Kotwica, Jan, E-mail: janko@pan.olsztyn.pl

2012-03-01T23:59:59.000Z

52

Berthon P, Katoh M, Dusanter-Fourt 1, Kelly PA, Djiane J, 1986b. Purification of prolactin receptor from sow mam-mary gland and polyclonal antibodies production. Mol Cell Endocrinol, soumis publication  

E-Print Network [OSTI]

Berthon P, Katoh M, Dusanter-Fourt 1, Kelly PA, Djiane J, 1986b. Purification of prolactin receptor publication Djiane J, Durand P, Kelly PA, 1977. Evolution of prolactin receptors in rabbit mammary gland during pregnancy and lactation. Endocrinology, 100:1348-1356 Djiane J, Dusanter-Fourt 1, Katoh M, Kelly

Paris-Sud XI, Université de

53

accelerates mammary tumorprogression: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the mammary gland is well known. However, it is not well established. To determine where PRL could exert its effects within the mammary gland, we investigated the levels Kihara,...

54

accelerates mammary oncogenesis: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the mammary gland is well known. However, it is not well established. To determine where PRL could exert its effects within the mammary gland, we investigated the levels Kihara,...

55

alpha positive mammary: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the mammary gland is well known. However, it is not well established. To determine where PRL could exert its effects within the mammary gland, we investigated the levels Kihara,...

56

BMP4 sufficiency to induce choroid plexus epithelial fate from embryonic stem cell-derived neuroepithelial progenitors  

E-Print Network [OSTI]

incubated with 8 uM Vybrant CFDA-SE Cell Tracker dye (LifeRiver) followed by Vybrant CFDA-SE dye labeling as describedonly those cells that were CFDA-SE/Hoechst colabelled and

2012-01-01T23:59:59.000Z

57

E-Print Network 3.0 - adult esophageal epithelial Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

However, epithelial injury... potential mechanisms regulating basal cells and allows comparison with other ... Source: Hogan, Brigid L.M. - Department of Cell Biology, Duke...

58

The recruitment of stromal cells to the site of tumor formation  

E-Print Network [OSTI]

Myofibroblasts are an alpha-smooth muscle actin ([alpha]-SMA)-expressing cell type found within human mammary carcinomas, but not in the normal mammary gland. Myofibroblasts can enhance tumor formation by promoting ...

Saelzler, Matthew P. (Matthew Paul)

2010-01-01T23:59:59.000Z

59

Stromal cell-derived factor-1 overexpression induces gastric dysplasia through expansion of stromal myofibroblasts and epithelial progenitors  

E-Print Network [OSTI]

Objective: Stromal cell-derived factor-1 (SDF-1/CXCL12), the main ligand for CXCR4, is overexpressed in human cancer. This study addressed the precise contribution of SDF-1 to gastric carcinogenesis.

Shibata, Wataru

60

Regulation of mammary gland remodelling and lactation  

E-Print Network [OSTI]

and their expression, organization and physico-chemical properties of casein micelle, and subcellular transport not induce any insulin resistance, but it improves the insulin- stimulated glucose disposal in lactating presented: laser scanning cytometry for mammary apoptosis research, septaplex PCR for "parent- age control

Paris-Sud XI, Université de

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Antioxidant and Anti-Inflamatory Effects and Mechanisms of Green Tea in Vitro in Vascular Epithelial Cells  

E-Print Network [OSTI]

or lone electrons. These electrons are reactive and have the capability to cause damage to tissues if left in their unstable state. In order to counteract these free radicals there are antioxidants which seek out the free radicals and bind to them... Cells. (April 2009) Abida Hasan Department of Nutritional Sciences Texas A&M University Research Advisor: Dr. Susanne Talcott Department of Nutritional Sciences In the human body there are free radicals present which have one or many unpaired...

Hasan, Abida

2011-08-04T23:59:59.000Z

62

Intracellular trafficking and plasma membrane microdomain distribution of the NSP4 enterotoxin during rotavirus infection in epithelial cells  

E-Print Network [OSTI]

Sterol Transfer from Detergent-Resistant Membranes (DRM) and Detergent-Soluble Membranes (DSM) ????????????? 31 5 Sterol Transfer from Non-detergent Caveolae/Rafts ???.?............. 34 6 Sterol Transfer from Affinity-purified Caveolae... pups and pup intestinal epithelia induced diarrhea and fluid accumulation with chloride secretion, respectively (Ball et al., 1996). Addition of purified NSP4 to HT-29 cells induces a rapid rise in phospholipase C (PLC)-catalyzed inositiol 1...

Storey, Stephen Michael

2009-05-15T23:59:59.000Z

63

3,5,4?-Trimethoxystilbene, a natural methoxylated analog of resveratrol, inhibits breast cancer cell invasiveness by downregulation of PI3K/Akt and Wnt/?-catenin signaling cascades and reversal of epithelial–mesenchymal transition  

SciTech Connect (OSTI)

The molecular basis of epithelial–mesenchymal transition (EMT) functions as a potential therapeutic target for breast cancer because EMT may endow breast tumor-initiating cells with stem-like characteristics and enable the dissemination of breast cancer cells. We have recently verified the antitumor activity of 3,5,4?-trimethoxystilbene (MR-3), a naturally methoxylated derivative of resveratrol, in colorectal cancer xenografts via an induction of apoptosis. The effect of MR-3 on EMT and the invasiveness of human MCF-7 breast adenocarcinoma cell line were also explored. We found that MR-3 significantly increased epithelial marker E-cadherin expression and triggered a cobblestone-like morphology of MCF-7 cells, while reciprocally decreasing the expression of mesenchymal markers, such as snail, slug, and vimentin. In parallel with EMT reversal, MR-3 downregulated the invasion and migration of MCF-7 cells. Exploring the action mechanism of MR-3 on the suppression of EMT and invasion indicates that MR-3 markedly reduced the expression and nuclear translocation of ?-catenin, accompanied with the downregulation of ?-catenin target genes and the increment of membrane-bound ?-catenin. These results suggest the involvement of Wnt/?-catenin signaling in the MR-3-induced EMT reversion of MCF-7 cells. Notably, MR-3 restored glycogen synthase kinase-3? activity by inhibiting the phosphorylation of Akt, the event required for ?-catenin destruction via a proteasome-mediated system. Overall, these findings indicate that the anti-invasive activity of MR-3 on MCF-7 cells may result from the suppression of EMT via down-regulating phosphatidylinositol 3-kinase (PI3K)/AKT signaling, and consequently, ?-catenin nuclear translocation. These occurrences ultimately lead to the blockage of EMT and the invasion of breast cancer cells. - Highlights: • MR-3 blocked MCF-7 cell invasion by inducing a reversal of EMT. • Wnt/?-catenin signaling is involved in MR-3-induced EMT reversion of MCF-7 cells. • Knockdown of ?-catenin was sufficient to restore epithelial marker E-cadherin levels. • MR-3 recovered the function of GSK-3? that inhibits ?-catenin nuclear translocation.

Tsai, Jie-Heng [Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 402, Taiwan, ROC (China); Hsu, Li-Sung [Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 402, Taiwan, ROC (China); Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 402, Taiwan, ROC (China); Lin, Chih-Li [Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, ROC (China); Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402, Taiwan, ROC (China); Hong, Hui-Mei [Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402, Taiwan, ROC (China); Department of Biomedical Sciences, Chung Shan Medical University, Taichung 402, Taiwan, ROC (China); Pan, Min-Hsiung [Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, ROC (China); Way, Tzong-Der [Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung 40402, Taiwan, ROC (China); Chen, Wei-Jen, E-mail: cwj519@csmu.edu.tw [Department of Medical Research, Chung Shan Medical University Hospital, Taichung 402, Taiwan, ROC (China); Department of Biomedical Sciences, Chung Shan Medical University, Taichung 402, Taiwan, ROC (China)

2013-11-01T23:59:59.000Z

64

Inhibition of ICAM-1/LFA-1-mediated Heterotypic T-cell Adhesion to Epithelial Cells: Design of ICAM-1 Cyclic Peptides  

E-Print Network [OSTI]

In this work, we have designed cyclic peptides (cIBL, cIBR, cIBC, CH4 and CH7) derived from the parent IB peptide (ICAM-11–21) that are inhibitors of ICAM-1/LFA-1-mediated T-cell adhesion to Caco-2 cell monolayers. Cyclic ...

Anderson, Meagan E.; Yakovleva, Tatyana; Yongbo, Hu; Siahaan, Teruna J.

2004-03-01T23:59:59.000Z

65

E-Print Network 3.0 - advanced ovarian epithelial Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

as an animal model of human epithelial ovarian cancer (15, 26). ... Source: Mayo, Kelly E. - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern...

66

E-Print Network 3.0 - advanced epithelial ovarian Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

as an animal model of human epithelial ovarian cancer (15, 26). ... Source: Mayo, Kelly E. - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern...

67

E-Print Network 3.0 - activation disrupts epithelial Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

185-194 Plasticity of epithelial cell shape in response... expresses enhanced green fluorescent protein (eGFP) specifically in the ectodermal ... Source: Bosch, Thomas C. G....

68

Differentiation and functional maturation of bone marrow-derived intestinal epithelial T cells expressing membrane T cell receptor in athymic radiation chimeras  

SciTech Connect (OSTI)

The thymus dependency of murine intestinal intraepithelial lymphocytes (IEL) was studied in an athymic F1----parent radiation chimera model. IEL, although not splenic or lymph node lymphocytes, from athymic chimeras displayed normal levels of cells bearing the class-specific T cell Ag, CD4 and CD8; the TCR-associated molecule, CD3; and the Thy-1 Ag. Moreover, two-color flow cytometric analyses of IEL from athymic mice demonstrated regulated expression of T cell Ag characteristic of IEL subset populations from thymus-bearing mice. In immunoprecipitation experiments, surface TCR-alpha beta or TCR-gamma delta were expressed on IEL, although not on splenic lymphocytes, from athymic chimeras. That IEL from athymic chimeras constituted a population of functionally mature effector cells activated in situ, similar to IEL from thymus-bearing mice, was demonstrated by the presence of CD3-mediated lytic activity of athymic lethally irradiated bone marrow reconstituted IEL. These data provide compelling evidence that intestinal T cells do not require thymic influence for maturation and development, and demonstrate that the microenvironment of the intestinal epithelium is uniquely adapted to regulate IEL differentiation.

Mosley, R.L.; Styre, D.; Klein, J.R. (Univ. of Tulsa, OK (USA))

1990-09-01T23:59:59.000Z

69

antigen-driven mammary carcinoma: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the mammary gland is well known. However, it is not well established. To determine where PRL could exert its effects within the mammary gland, we investigated the levels Kihara,...

70

Increased differentiation properties in two- and three-dimensional coculture of hepatocytes and liver epithelial cells by a novel quantitative functional liver assay  

E-Print Network [OSTI]

Hepatic stem cells in adult rats are activated by chemical injury to the liver, causing hepatic progenitor cells to proliferate, integrate into the hepatic plates, and differentiate into hepatocytes. In an attempt to model ...

Moritz, Joseph M. (Joseph Michael)

2007-01-01T23:59:59.000Z

71

Original article Sensitization of the bovine mammary gland to  

E-Print Network [OSTI]

(ReceivedI October I )96; accepted 4 February 1997) Summary ― The effect of repeated infusions intramammary infusions of E cnli endotoxin (33 pg) 24 h apart in the same mammary quarter. Along with the second infusion, the cows received one dose of endotoxin in the contralateral quarter. Milk was collected

Boyer, Edmond

72

REVIEW Open Access Epithelial to mesenchymal transition as a biomarker  

E-Print Network [OSTI]

subjected to injury may undergo similar transformations and thus provide new fibroblasts in the interstitium epithelial cells aberrantly expressing fibroblast-specific protein (FSP)1 in a model of mouse anti-tubular membrane disease [4]. This led Strutz et al. to hypothesize that some fibro- blasts might be derived from

Boyer, Edmond

73

E-Print Network 3.0 - alveolar epithelial gp60 Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of the underlying mammary mesenchyme by the presumptive mammary epithelium to establish a bulb of ... Source: Scott, Matthew - Departments of Developmental Biology, Genetics, &...

74

Mammary stem and progenitor cells: Tumour precursors? Amy Paguirigana  

E-Print Network [OSTI]

.01.048 * Corresponding author: Tel.: +1 608 265 5182; fax: +1 608 262 2824. E-mail address: alexander

Beebe, David J.

75

Characterisation of microRNA expression in post-natal mouse mammary gland development  

E-Print Network [OSTI]

. For example during puberty and gestation, proliferation and associated processes of cell division and mitosis were highly represented, cal- cium/sodium ion transport, translation and intracellular protein transport were prominent during lactation, apop- tosis... and miR-429. There is increasing evidence that this miRNA family plays a crucial role in the regulation of epithelial to mesenchy- mal transition (EMT). All five members of the miR-200 family were markedly down-regulated in cells that had undergone EMT...

Avril-Sassen, Stefanie; Goldstein, Leonard D; Stingl, John; Blenkiron, Cherie; Le Quesne, John; Spiteri, Inmaculada; Karagavriilidou, Konstantina; Watson, Christine J; Tavare, Simon; Miska, Eric A; Caldas, Carlos

2009-11-20T23:59:59.000Z

76

S14 protein in breast cancer cells: Direct evidence of regulation by SREBP-1c, superinduction with progestin, and effects on cell growth  

SciTech Connect (OSTI)

Most breast cancers exhibit brisk lipogenesis, and require it for growth. S14 is a lipogenesis-related nuclear protein that is overexpressed in most breast cancers. Sterol response element-binding protein-1c (SREBP-1c) is required for induction of lipogenesis-related genes, including S14 and fatty acid synthase (FAS), in hepatocytes, and correlation of SREBP-1c and FAS expression suggested that SREBP-1c drives lipogenesis in tumors as well. We directly tested the hypothesis that SREBP-1c drives S14 expression and mediates lipogenic effects of progestin in T47D breast cancer cells. Dominant-negative SREBP-1c inhibited induction of S14 and FAS mRNAs by progestin, while active SREBP-1c induced without hormone and superinduced in its presence. Changes in S14 mRNA were reflected in protein levels. A lag time and lack of progestin response elements indicated that S14 and FAS gene activation by progestin is indirect. Knockdown of S14 reduced, whereas overexpression stimulated, T47D cell growth, while nonlipogenic MCF10a mammary epithelial cells were not growth-inhibited. These data directly demonstrate that SREBP-1c drives S14 gene expression in breast cancer cells, and progestin magnifies that effect via an indirect mechanism. This supports the prediction, based on S14 gene amplification and overexpression in breast tumors, that S14 augments breast cancer cell growth and survival.

Martel, Peter M. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Bingham, Chad M. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); McGraw, Charles J. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Baker, Christina L. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Morganelli, Peter M. [Department of Microbiology and Immunology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Meng, Marie Louise [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Norris Cotton Cancer Center, Dartmouth Medical School (United States); Armstrong, Jessica M. [Norris Cotton Cancer Center, Dartmouth Medical School (United States); Department of Physiology, Dartmouth Medical School (United States); Moncur, Joel T. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States); Kinlaw, William B. [Department of Medicine, Division of Endocrinology, Dartmouth Medical School (United States) and Norris Cotton Cancer Center, Dartmouth Medical School (United States)]. E-mail: william.kinlaw@hitchcock.org

2006-02-01T23:59:59.000Z

77

Effect of Intravenous Amino Acid Infusion on Leucine Oxidation Across the Mammary Gland  

E-Print Network [OSTI]

Effect of Intravenous Amino Acid Infusion on Leucine Oxidation Across the Mammary Gland) and the AA infusion periods. Although blood flow to the mammary gland and the arterial concen- tration of most AA other than leucine were increased by the AA infusion, milk and protein yields did not change

Bequette, Brian J.

78

The Open Breast Cancer Journal, 2011, 3, 31-44 31 1876-8172/11 2011 Bentham Open  

E-Print Network [OSTI]

in breast normal cells. The breast cancer cell lines HCC1419, MCF7, MDA-MB-231, MDA-MB-468, SKBR3, the normal mammary epithelial cell line HME 50 HT and the normal mammary fibroblast cell line CCD-1074sk were of the chemotherapeutics on the normal cells and tissues. Trastuzumab, the newest anticancer treatment, targets cancer

Park, Jong-Sang

79

Response of an experimental mammary carcinoma to fractionated x-irradiation with misonidazole and microwave hyperthermia  

SciTech Connect (OSTI)

X/Gf mice bearing the MT2 mammary adenocarcinoma were subjected to 4000 rad of x rays given either as a single dose, or five daily fractions of 800 rad. Additional experimental groups were treated with either short term localized microwave hyperthermia (LMH), or the hypoxic cell radiosensitizer misonidazole (MISO), or both hyperthermia plus MISO with x rays. The combined use of MISO plus 42.5/sup 0/C with x rays was superior to the other treatment regimens as assessed by tumor regrowth delay and mean survival time. However, for the five fraction schedule, the addition of MISO plus hyperthermia was not as effective as observed for the single dose treatment. This may be attributed to reoxygenation of the hypoxic tumor cells between treatment fractions. MISO retention in tumor tissue under ambient and hyperthermic conditions was studied. The application of heat locally to the tumors caused a significant increase in MISO tumor concentration. However, after four x ray fractions the influence on MISO concentration by hyperthermia in the tumors could not be demonstrated.

Goldfeder, A.; Brown, D.M.

1984-08-01T23:59:59.000Z

80

Extracellular matrix signatures of human mammary carcinoma identify novel metastasis promoters  

E-Print Network [OSTI]

The extracellular matrix (ECM) is a major component of tumors and a significant contributor to cancer progression. In this study, we use proteomics to investigate the ECM of human mammary carcinoma xenografts and show that ...

Naba, Alexandra

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

E-Print Network 3.0 - a431 cells overexpressing Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

nanoparticles Summary: , "Consumption of EGF by A431 cells: evidence for receptor recycling," J. Cell. Biol. 120, 85-93 (1993). 12. K... epithelial carcinoma cells (A431...

82

Annexin A9 (ANXA9) biomarker and therapeutic target in epithelial cancer  

DOE Patents [OSTI]

Amplification of the ANXA9 gene in human chromosomal region 1q21 in epithelial cancers indicates a likelihood of both in vivo drug resistance and metastasis, and serves as a biomarker indicating these aspects of the disease. ANXA9 can also serve as a therapeutic target. Interfering RNAs (iRNAs) (such as siRNA and miRNA) and shRNA adapted to inhibit ANXA9 expression, when formulated in a therapeutic composition, and delivered to cells of the tumor, function to treat the epithelial cancer.

Hu, Zhi (El Cerrito, CA); Kuo, Wen-Lin (San Ramon, CA); Neve, Richard M. (San Mateo, CA); Gray, Joe W. (San Francisco, CA)

2012-06-12T23:59:59.000Z

83

E-Print Network 3.0 - alveolar type-ii cell Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

adhesion sites and collagen degradation peptides could induce alveolar type II... M CaCl2. Cell cultures Isolation and primary culture of alveolar epithelial cells Alveolar ......

84

Expression of butyrophilin (Btn1a1) in lactating mammary gland is essential for the regulated  

E-Print Network [OSTI]

secretion of milk­lipid droplets Sherry L. Ogg*, Anne K. Weldon*, Lorraine Dobbie , Andrew J. H. Smith expressed in the lactating mammary gland and is secreted into milk in association with lipid droplets expression of Btn1a1 was either disrupted or eliminated, respectively. The regulated secretion of milk­lipid

Mather, Ian

85

Treatment of an Iatrogenic Left Internal Mammary Artery to Pulmonary Artery Fistula with a Bovine Pericardium Covered Stent  

SciTech Connect (OSTI)

We report a case with an acquired fistula between the left internal mammary artery and the pulmonary artery following coronary bypass surgery treated with a bovine pericardium covered stent. We also reviewed similar cases reported previously.

Heper, Gulumser [SSK Ihtisas Hospital, Department of Cardiology (Turkey)], E-mail: heperg@hotmail.com; Barcin, Cem; Iyisoy, Atila; Tore, Hasan F. [Gulhane Military Medical Academy, Department of Cardiology (Turkey)

2006-10-15T23:59:59.000Z

86

Purification of PRL receptors from toad kidney: Comparisons with rabbit mammary PRL receptors  

SciTech Connect (OSTI)

The binding characteristics of the prolactin (PRL) receptors present in toad (Bufo marinus) kidneys were investigated and compared to those of PRL receptors present in rabbit mammary glands. The molecular characteristics of the Triton X-100 solubilized renal and mammary PRL receptors were assessed by gel filtration and by migration analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after affinity labeling of the binding sites with {sup 125}I-human growth hormone. Similar results were obtained for both receptors. Partial purification of the toad PRL receptor could be achieved by affinity chromatography. The molecular weight of this purified receptor could be determined by analysis of SDS-PAGE. With the use of a polyclonal antiserum raised against a purified preparation of rabbit mammary PRL receptor, one or several antigenic epitope(s) could be identified on the core of the toad renal PRL receptor. In conclusion, although the structure and the biological role(s) of PRL have substantially changed during evolution, the receptor for this hormone has retained many of its structural features as could be assessed between an amphibian and a mammalian species on functionally different target tissues.

Dunand, M.; Kraehenbuhl, J.P.; Rossier, B.C.; Aubert, M.L. (Univ. of Geneva School of Medicine (Switzerland) Univ. of Lausanne School of Medicine (Switzerland))

1988-03-01T23:59:59.000Z

87

E-Print Network 3.0 - adult stromal cells Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the interactions between stromal and epithelial cells in tumorigenesis. Time-lapse fluorescent videos using mouse... interactions This session focused on reciprocal...

88

E-Print Network 3.0 - alveolar bone-derived cells Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Respiratory Physiology 2 Pulmonary Mechanics Summary: , 3rd Ed. Fig. 10.2 12;Surfactant Secretion by Type II Alveolar Epithelial Cells from multilamellar... basal laminabasement...

89

The effect of scaffold physical properties on endothelial cell function  

E-Print Network [OSTI]

Endothelial cells (EC) are ubiquitous - as vascular epithelial cells they line the inner surface of all vessels and are the contact surface with flowing blood. Macrovascular EC are the first line barrier between flowing ...

Murikipudi, Sylaja

2010-01-01T23:59:59.000Z

90

Partial proteolytic digestion of the mammary prolactin receptor: Identification of smaller prolactin binding fragments  

SciTech Connect (OSTI)

Partial proteolytic digestion of the mammary prolactin (PRL) receptor was used to generate receptor fragments and analyze their immunoreactivity and PRL binding properties. Tryptic digestion of the PRL receptor produced two immunoreactive fragments (Mr approximately 30,000 and approximately 15,000) that reacted with a monoclonal anti-PRL receptor antibody and still specifically bound PRL, while the complete immunoreactive PRL binding unit (Mr approximately 42,000) disappeared. Neither chymotrypsin nor V8 protease were able to generate any immunoreactive receptor fragments. These receptor fragments may represent smaller PRL binding receptor form(s) of biological significance.

Dusanter-Fourt, I.; Kelly, P.A.; Djiane, J. (Institut National de la Recherche Agronomique, Jouy-en-Josas (France))

1990-01-01T23:59:59.000Z

91

Comparative promoter activities of three endogenous copies of mouse mammary tumor virus  

E-Print Network [OSTI]

to 12 CC ~ o c 0 tu x MMTV M13 pLC1 ?(, a~ CAT pBR322 0/ I SV40 Figure 5. Diagram of pLC1. The plasmid pLC1 (Toohey et al. , 1986) contains an Intact LTR obtained from the C3H provirus as a Pst I restriction fragment. The plasmid also...COMPARATIVE PROMOTER ACTIVITIES OF THREE ENDOGENOUS COPIES OF MOUSE MAMMARY TUMOR VIRUS A Thesis by LAURA REID VILANDER Submitted to the Graduate College of Texas A&M University in partial fulfilment of the requirements for the degree...

Vilander, Laura Reid

1987-01-01T23:59:59.000Z

92

Apical polarity in three-dimensional culture systems: where to now?  

SciTech Connect (OSTI)

Delineation of the mechanisms that establish and maintain the polarity of epithelial tissues is essential to understanding morphogenesis, tissue specificity and cancer. Three-dimensional culture assays provide a useful platform for dissecting these processes but, as discussed in a recent study in BMC Biology on the culture of mammary gland epithelial cells, multiple parameters that influence the model must be taken into account.

Inman, J.L.; Bissell, Mina

2010-01-21T23:59:59.000Z

93

Epithelial cell polarity and proliferation control in Drosophila melanogaster  

E-Print Network [OSTI]

divergence led to its adoption into the recycling pathway,led to more complete models of apicobasal protein sorting in the trans-Golgi network (TGN) and recycling

Morrison, Holly Ann

2010-01-01T23:59:59.000Z

94

Prolactin and aging: X-irradiated and estrogen-induced rat mammary tumorigenesis  

SciTech Connect (OSTI)

Both sexes of inbred WF rats at either 8 or 28-60 weeks of age were exposed to 200 rad whole-body radiation, 2.5 or 5.0 mg 17 beta-estradiol (E2), or both agents The female rats treated with E2 alone or with both X-rays and E2 at 8 weeks of age showed a high incidence of mammary carcinomas (MCA), a large increase in pituitary weight, and a rise in serum prolactin (PRL) levels. However, the same treatments to males did not induce MCA despite a moderate increase in both pituitary weight and serum PRL. Ovariectomy prior to E2 treatment failed to modify the occurrence of MCA or pituitary tumors. When X-rays and E2 were given to female rats at 28-60 weeks of age, pituitary weight, serum PRL levels, and the incidence of MCA were unaffected. When the E2 pellet was kept for the first 24 weeks and withdrawn during the last 12 weeks, the incidence of MCA, pituitary weight, and serum PRL was low. It was concluded that: 1) the pituitary glands of young female rats were susceptible to E2 treatment but were insensitive in older females, and 2) the occurrence of MCA in female rats appeared to be promoted by elevated PRL levels secreted by E2-induced pituitary tumors. Mammary tissue of male rats was less sensitive to PRL levels in the development of MCA.

Ito, A.; Naito, M.; Watanabe, H.; Yokoro, K.

1984-07-01T23:59:59.000Z

95

Biological activities of binding site specific monoclonal antibodies to prolactin receptors of rabbit mammary gland  

SciTech Connect (OSTI)

The biological activity of three monoclonal antibodies (mAbs) against the rabbit mammary prolactin (PRL) receptor (M110, A82, and A917) were investigated using explants of rabbit mammary gland. The three mAbs which were all able to inhibit the binding of SVI-ovine prolactin to its receptor had different biological activities. Two mAbs (M110 and A82) were able to prevent the stimulating effect of PRL on casein synthesis when the molar ratio between the mAb and PRL was 100. One mAb (A917) was able to mimic the action of PRL on both casein and DNA ((TH)thymidine incorporation) synthesis, whereas the other two mAbs were without any stimulatory effect. For this stimulatory effect to be observed, bivalency of the antibody was essential, since monovalent fragments, which were able to inhibit PRL binding, had no agonistic activity. The ability of the mAbs to induce a down-regulation of receptors was also studied. These studies suggest that the binding domain of the receptor might be relatively complex, since only a part of this domain recognized by the antibody with PRL-like activity was able to induce hormonal action. Alternatively, only those antibodies able to microaggregate the receptors may possess PRL-like activity.

Djiane, J.; Dusanter-Fourt, I.; Katoh, M.; Kelly, P.A.

1985-09-25T23:59:59.000Z

96

Oestrogen metabolism and action in epithelial ovarian cancer   

E-Print Network [OSTI]

Ovarian cancer is the most fatal of all gynecological malignancies. Epithelial ovarian cancer (EOC) accounts for about 90% of malignant ovarian tumours and is thought to originate mostly from ovarian surface epithelium ...

Ren, Xia

2011-11-25T23:59:59.000Z

97

Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture  

SciTech Connect (OSTI)

CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1), a type I transmembrane glycoprotein involved in cell-cell adhesion has been shown to act as an angiogenic factor for mouse and human endothelial cells. Based on the ability of CEACAM1 to initiate lumen formation in human mammary epithelial cells grown in 3D culture (Matrigel), we hypothesized that murine CEACAM1 may play a similar role in vasculogenesis. In order to test this hypothesis, murine embryonic stem (ES) cells stimulated with VEGF were differentiated into embryoid bodies (EB) for 8 days (- 8-0 d) and transferred to Matrigel in the presence or absence of anti-CEACAM1 antibody for an additional 12 days (0-12 d). In the absence of anti-CEACAM1 antibody or in the presence of an isotype control antibody, the EB in Matrigel underwent extensive sprouting, generating lengthy vascular structures with well-defined lumina as demonstrated by confocal microscopy, electron microscopy, and immunohistochemical analysis. Both the length and architecture of the vascular tubes were inhibited by anti-CEACAM1 mAb CC1, a mAb that blocks the cell-cell adhesion functions of CEACAM1, thus demonstrating a critical role for this cell-cell adhesion molecule in generating and maintaining vasculogenesis. QRT-PCR analysis of the VEGF treated ES cells grown under conditions that convert them to EB revealed expression of Ceacam1 as early as - 5 to - 3 d reaching a maximum at day 0 at which time EBs were transferred to Matrigel, thereafter levels at first declined and then increased over time. Other markers of vasculogenesis including Pecam1, VE-Cad, and Tie-1 were not detected until day 0 when EBs were transferred to Matrigel followed by a steady increase in levels, indicating later roles in vasculogenesis. In contrast, Tie-2 and Flk-1 (VEGFR2) were detected on day five of EB formation reaching a maximum at day 0 on transfer to Matrigel, similar to Ceacam1, but after which Tie-2 declined over time, while Flk-1 increased over time. QRT-PCR analysis of the anti-CEACAM1 treated ES cells revealed a significant decrease in the expression of Ceacam1, Pecam1, Tie-1, and Flk-1, while VE-Cad and Tie-2 expression were unaffected. These results suggest that the expression and signaling of CEACAM1 may affect the expression of other factors known to play critical roles in vasculogenesis. Furthermore this 3D model of vasculogenesis in an environment of extracellular matrix may be a useful model for comparison to existing models of angiogenesis.

Gu, Angel [Department of Immunology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010 (United States)] [Department of Immunology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010 (United States); Tsark, Walter [Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010 (United States)] [Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010 (United States); Holmes, Kathryn V. [Department of Microbiology, University of Colorado Health Sciences, Aurora, CO 80045 (United States)] [Department of Microbiology, University of Colorado Health Sciences, Aurora, CO 80045 (United States); Shively, John E., E-mail: jshively@coh.org [Department of Immunology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010 (United States)

2009-06-10T23:59:59.000Z

98

Concentration of PCBs,HCB,DDT, and HCH isomers in the ovaries, mammary gland, and liver of cows  

SciTech Connect (OSTI)

Persistent organic chlorine compounds such as DDT and its metabolites, hexachlorobenzene (HCB) and polychlorinated biphenyls (PCBs) play an important role in chronic poisoning and take part in a number of pathological processes. This study estimates the degree of accumulation of organic Chlorine compounds and polychlorinated biphynyls in the liver, ovaries, and mammary gland tissues of cows.12 refs., 1 fig., 1 tab.

Sitarska, E.; Klucinski, W.; Faundez, R. [Agricultural Univ. of Warsaw (Poland)]|[National Inst. of Hygiene, Warsaw (Poland)] [and others

1995-12-01T23:59:59.000Z

99

Inhibitory actions of Ah receptor agonists and indole-containing compounds in breast cancer cell lines and mouse models  

E-Print Network [OSTI]

. Recently, it has been reported that the SAhRM 1,1??,2,2??-tetramethyldiindolylmethane inhibited DMBA-induced mammary tumor growth in rats and also inhibited MAPK and PI3-K pathways in human breast cancer cells. BT-474 and MDA-MB-453 cell lines are ErbB2...

Walker, Kelcey Manae Becker

2005-08-29T23:59:59.000Z

100

Regulation of Mammary cell Differentiation and Metabolism by Singleminded-2s  

E-Print Network [OSTI]

progression are poorly understood. Therefore, determining the mechanisms by which some DCIS progress is critical for future breast cancer diagnostics and treatment. Singleminded-2s (SIM2s) is a member of the bHLH/PAS family of transcription factors and a key...

Scribner, Kelly C

2013-05-21T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

The role of 3D microenvironmental organization in MCF-7 epithelial–mesenchymal transition after 7 culture days  

SciTech Connect (OSTI)

We present a multi-technique study on in vitro epithelial–mesenchymal transition (EMT) in human MCF-7 cells cultured on electrospun scaffolds of poly(L-lactic acid) (PLA), with random and aligned fiber orientations. Our aim is to investigate the morphological and genetic characteristics induced by extracellular matrix in tumor cells cultured in different 3D environments, and at different time points. Cell vitality was assessed with AlamarBlue at days 1, 3, 5 and 7. Scanning electron microscopy was performed at culture days 3 and 7. Immunohistochemistry (for E-cadherin, ?-catenin, cytokeratins, nucleophosmin, tubulin, Ki-67 and vimentin), immunofluorescence (for F-actin) western blot (for E-cadherin, ?-catenin and vimentin) and transmission electron microscopy were carried out at day 7. An EMT gene array followed by PCR analysis confirmed the regulation of selected genes. At day 7, scanning electron microscopy on aligned-PLA revealed spindle-shaped cells gathered in buds and ribbon-like structures, with a higher nucleolar/nuclear ratio and a loss in E-cadherin and ?-catenin at immunohistochemistry and western blot. An up-regulation of SMAD2, TGF-?2, TFPI2 and SOX10 was found in aligned-PLA compared to random-PLA cultured cells. The topography of the extracellular matrix has a role in tumor EMT, and a more aggressive phenotype characterizes MCF-7 cells cultured on aligned-PLA scaffold. -- Highlights: • After 7 culture days an aligned-PLA scaffold induces a spindle shape to MCF-7 cells. • Despite these changes, the aligned MCF-7 cells keep an epithelial phenotype. • The extracellular environment alone influences the E-cadherin/?-catenin axis. • The extracellular environment can promote the epithelial–mesenchymal transition.

Foroni, Laura [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Vasuri, Francesco, E-mail: vasurifrancesco@libero.it [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Chair of Vascular Surgery, Department of Specialistic Surgery and Anaesthesiological Sciences, S. Orsola-Malpighi Hospital, Bologna University (Italy); Valente, Sabrina [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Gualandi, Chiara [Department of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, RU Bologna), Bologna University (Italy); Focarete, Maria Letizia [Department of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, RU Bologna), Bologna University (Italy); Health Science and Technologies–Interdepartmental Center for Industrial Research (HST-ICIR), Bologna University (Italy); Caprara, Giacomo [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy); Scandola, Mariastella [Department of Chemistry “G. Ciamician” and National Consortium of Materials Science and Technology (INSTM, RU Bologna), Bologna University (Italy); D'Errico-Grigioni, Antonia; Pasquinelli, Gianandrea [Pathology Unit, Department of Haematology, Oncology and Clinical Pathology, S. Orsola-Malpighi Hospital, Bologna University (Italy)

2013-06-10T23:59:59.000Z

102

Restoration of normal phenotype in cancer cells  

DOE Patents [OSTI]

A method for reversing expression of malignant phenotype in cancer cells is described. The method comprises applying {beta}{sub 1} integrin function-blocking antibody to the cells. The method can be used to assess the progress of cancer therapy. Human breast epithelial cells were shown to be particularly responsive. 14 figs.

Bissell, M.J.; Weaver, V.M.

1998-12-08T23:59:59.000Z

103

Restoration of normal phenotype in cancer cells  

DOE Patents [OSTI]

A method for reversing expression of malignant phenotype in cancer cells is described. The method comprises applying .beta..sub.1 integrin function-blocking antibody to the cells. The method can be used to assess the progress of cancer therapy. Human breast epithelial cells were shown to be particularly responsive.

Bissell, Mina J. (Berkeley, CA); Weaver, Valerie M. (Oakland, CA)

1998-01-01T23:59:59.000Z

104

Quantitative studies of EGFR autocrine induced cell signaling and migration  

E-Print Network [OSTI]

Epidermal growth factor (EGF) receptor autocrine and/or paracrine signaling plays an important role in normal epithelial cell proliferation, survival, adhesion and migration. Aberrant expression of the EGF receptor and its ...

Joslin, Elizabeth Jane

2007-01-01T23:59:59.000Z

105

affects host cell: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Biology and Medicine Websites Summary: Figure S1, related to Figure 2. M1T15448 GAS replicate efficiently in the cytosol of epithelial cells replication of GAS. (A)...

106

Mechanisms of hormonal regulation of CAD gene expression and inhibition by Aryl hydrocarbon receptor agonist in human breast cancer cells  

E-Print Network [OSTI]

-mediated pathway. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands suppress several E2-induced responses in the rodent uterus and mammary tumors and in human breast cancer cells. TCDD inhibited hormone...

Khan, Shaheen Munawar Ali

2007-04-25T23:59:59.000Z

107

Effect of prolactin on enzymes of lipid biosynthesis in mammary gland explants  

SciTech Connect (OSTI)

Prolactin (PRL) stimulates an increased rate of incorporation of ({sup 14}C)acetate and ({sup 3}H)glucose into lipids in cultured mammary gland explants from 10-to 14-day-pregnant mice. This response is biphasic with an early increase occurring from 6 through 12 h, and an additional increase from 16 to 24 h. Enzymes likely to be rate limiting to this process include acetyl CoA carboxylase, fatty acid synthetase, acetyl CoA synthetase, and/or pyruvate dehydrogenase. Of these enzymes only pyruvate dehydrogenase activity was elevated at 6 h, suggesting that this enzymatic activity is important in stimulating early increases in lipogenesis after PRL treatment. In addition, the PRL stimulation of pyruvate dehydrogenase may also indirectly stimulate acetyl CoA carboxylase through the generation of citrate; this may explain the early (6-12 h) effect of PRL on ({sup 14}C)acetate incorporation. After 16 h of PRL treatment, the activities of all the lipogenic enzymes were enhanced. The second phase of PRLs stimulation of lipogenesis thus likely involves the enhanced activities of more than one of the lipogenic enzymes.

Waters, S.B.; Rillema, J.A. (Wayne State Univ. School of Medicine, Detroit, MI (USA))

1988-10-01T23:59:59.000Z

108

Internal Mammary Lymph Node Irradiation Contributes to Heart Dose in Breast Cancer  

SciTech Connect (OSTI)

We assessed the impact of internal mammary chain radiotherapy (IMC RT) to the radiation dose received by the heart in terms of heart dose-volume histogram (DVH). Thirty-six consecutive breast cancer patients presenting with indications for IMC RT were enrolled in a prospective study. The IMC was treated by a standard conformal RT technique (50 Gy). For each patient, a cardiac DVH was generated by taking into account the sole contribution of IMC RT. Cardiac HDV were compared according to breast cancer laterality and the type of previous surgical procedure, simple mastectomy or breast conservative therapy (BCT). The contribution of IMC RT to the heart dose was significantly greater for patients with left-sided versus right-sided tumors (13.8% and 12.8% for left-sided tumors versus 3.9% and 4.2% for right-sided tumors in the BCT group and the mastectomy group, respectively; p < 0.0001). There was no statistically significant difference in IMC contribution depending on the initial surgical procedure. IMC RT contributes to cardiac dose for both left-sided and right-sided breast cancers, although the relative contribution is greater in patients with left-sided tumors.

Chargari, Cyrus [Department of Radiotherapy, Institut Gustave Roussy, Villejuif (France); Department of Radiotherapy and Medical Oncology, Hopital d'Instruction des Armees du Val-de-Grace, Paris (France); Castadot, Pierre [Department of Radio-Oncology, Institut Jules Bordet, Brussels (Belgium); MacDermed, Dhara [Department of Radiation and Cellular Oncology, University of Chicago, Chicago, IL (United States); Vandekerkhove, Christophe [Department of Medical Physics, Institut Jules Bordet, Brussels (Belgium); Bourgois, Nicolas; Van Houtte, Paul [Department of Radio-Oncology, Institut Jules Bordet, Brussels (Belgium); Magne, Nicolas, E-mail: nicolas.magne@igr.f [Department of Radiotherapy, Institut Gustave Roussy, Villejuif (France); Department of Radio-Oncology, Institut Jules Bordet, Brussels (Belgium)

2010-10-01T23:59:59.000Z

109

10.1101/gr.108217.110Access the most recent version at doi: 2010 20: 1730-1739 originally published online November 2, 2010Genome Res.  

E-Print Network [OSTI]

and neoplastic mammary epithelial cell transcriptomes. We develop data analysis pipelines that allow the mapping is significantly higher than that of normal cells. Our analysis indicates that transcript discovery plateaus at 10. Comparison of SAGE-Seq and traditional SAGE on normal and cancerous breast tissues reveals higher sensitivity

Liu, Xiaole Shirley

110

Method for restoration of normal phenotype in cancer cells  

DOE Patents [OSTI]

A method for reversing expression of malignant phenotype in cancer cells is described. The method comprises applying .beta..sub.1 integrin function-blocking antibody to the cells. The method can be used to assess the progress of cancer therapy. Human breast epithelial cells were shown to be particularly responsive.

Bissell, Mina J. (Berkeley, CA); Weaver, Valerie M. (Oakland, CA)

2000-01-01T23:59:59.000Z

111

Sensitive Targeted Quantification of ERK Phosphorylation Dynamics and Stoichiometry in Human Cells without Affinity Enrichment  

SciTech Connect (OSTI)

Mass spectrometry-based targeted quantification is a promising technology for site-specific quantification of posttranslational modifications (PTMs). However, a major constraint of most targeted MS approaches is the limited sensitivity for quantifying low-abundance PTMs, requiring the use of affinity reagents to enrich specific PTMs. Herein, we demonstrate the direct site-specific quantification of ERK phosphorylation isoforms (pT, pY, pTpY) and their relative stoichiometries using a highly sensitive targeted MS approach termed high-pressure, high-resolution separations with intelligent selection and multiplexing (PRISM). PRISM provides effective enrichment of target peptides within a given fraction from complex biological matrix with minimal sample losses, followed by selected reaction monitoring (SRM) quantification. The PRISM-SRM approach enabled direct quantification of ERK phosphorylation in human mammary epithelial cells (HMEC) from as little as 25 µg tryptic peptides from whole cell lysates. Compared to immobilized metal-ion affinity chromatography, PRISM provided >10-fold improvement in signal intensities, presumably due to the better peptide recovery of PRISM for handling small size samples. This approach was applied to quantify ERK phosphorylation dynamics in HMEC treated by different doses of EGF at both the peak activation (10 min) and steady state (2 h). At 10 min, the maximal ERK activation was observed with 0.3 ng/mL dose, whereas the maximal steady state level of ERK activation at 2 h was at 3 ng/ml dose, corresponding to 1200 and 9000 occupied receptors, respectively. At 10 min, the maximally activated pTpY isoform represented ~40% of total ERK, falling to less than 10% at 2 h. The time course and dose-response profiles of individual phosphorylated ERK isoforms indicated that singly phosphorylated pT-ERK never increases significantly, while the increase of pY-ERK paralleled that of pTpY-ERK. This data supports for a processive, rather than distributed, model of ERK phosphorylation. The PRISM-SRM quantification of protein phosphorylation illustrates the potential for simultaneous quantification of multiple PTMs.

Shi, Tujin; Gao, Yuqian; Gaffrey, Matthew J.; Nicora, Carrie D.; Fillmore, Thomas L.; Chrisler, William B.; Gritsenko, Marina A.; Wu, Chaochao; He, Jintang; Bloodsworth, Kent J.; Zhao, Rui; Camp, David G.; Liu, Tao; Rodland, Karin D.; Smith, Richard D.; Wiley, H. S.; Qian, Weijun

2014-12-17T23:59:59.000Z

112

Interferon-? inhibits gastric carcinogenesis by inducing epithelial cell autophagy and T cell apoptosis  

E-Print Network [OSTI]

IFN-? mediates responses to bacterial infection and autoimmune disease, but it is also an important tumor suppressor. It is upregulated in the gastric mucosa by chronic Helicobacter infection; however, whether it plays a ...

Tu, Shui Ping

113

PRECLINICAL STUDY Prolonged mammosphere culture of MCF-7 cells induces an EMT  

E-Print Network [OSTI]

mammosphere culture conditions per se induced EMT in the epithelial MCF-7 breast cancer cell line. MCF- 7PRECLINICAL STUDY Prolonged mammosphere culture of MCF-7 cells induces an EMT and repression conditions in serum-supplemented media generated a cell population (called MCF-7M cells), which displays

Terasaki, Mark

114

A 3-D in vitro co-culture model of mammary gland involution  

E-Print Network [OSTI]

cells were allowed to establish in co-culture supplemented in maintenance media (+EGF 10ng/mL) for a further 3 days prior to lactogenic hormone differentiation with PRL, dexamethasone and insulin, for 2 weeks. At this stage KIM-2 cells formed... phenotype exploits the dependency of differentiated KIM-2 cells for prolactin (PRL) mediated survival signalling. Firstly we confirmed whether KIM-2 organoids regulate Stat3 and Stat5 phosphorylation in the same manner as the native gland, namely...

Campbell, Jonathan J.; Botos, Laur-Alexandru; Sargeant, Timothy J.; Davidenko, Natalia; Cameron, Ruth E.; Watson, Christine J.

2014-04-02T23:59:59.000Z

115

Stat3 controls cell death during mammary gland involution by regulating uptake of milk fat globules and lysosomal membrane permeabilization  

E-Print Network [OSTI]

with the indicated concentration of free fatty acids overnight and subsequently harvested and resuspended in 500 ?l culture medium. LysoTracker Red DND-99 (Life Technologies, 100 nM) was added to the suspension and incubated at 37 °C in the dark for 30 min. Single... using GraphPad Prism v4.0a. Supplementary Material Refer to Web version on PubMed Central for supplementary material. Acknowledgments We thank Helen Skelton for assistance with histology, Andrew Gilmore (University of Manchester, UK) for the Bax...

Sargeant, Timothy J.; Lloyd-Lewis, Bethan; Resemann, Henrike K.; Ramos-Montoya, Antonio; Skepper, Jeremy; Watson, Christine J.

2014-10-05T23:59:59.000Z

116

The role of ozone in tracheal cell transformation  

SciTech Connect (OSTI)

This project examined the potential role of ozone as a respiratory carcinogen by characterizing its ability to induce or modulate the preneoplastic transformation of rat tracheal epithelial cells. The chemical reactivity of ozone and the types of damage it can cause suggest that it may have a role in environmental carcinogenesis. Few other studies have examined the direct cytotoxic or transforming effects of ozone after in vivo or in vitro exposure of cells, and no studies have been reported on the comparative effects of ozone on respiratory cells exposed in vivo or in vitro. The induction of early preneoplastic changes in populations of rat tracheal epithelial cells by carcinogens can be detected and quantified in vitro after exposures in vivo or in vitro of tracheal epithelial cells. This cell culture and transformation system was used to characterize the transforming potency of ozone. Tracheal epithelial cells were isolated from Fischer-344/N rats that had been exposed for six hours per day, five days per week for one, two, or four weeks to 0, 0.12, 0.5, or 1.0 parts per million (ppm)* ozone (sea-level equivalents). Cell populations were examined in culture for increases in the frequency of preneoplastic variants. Rats exposed to ozone did not exhibit an increase in the frequency of preneoplastic tracheal cells, although exposed tracheas did exhibit dose-dependent morphological changes. Rat tracheal epithelial cells were given single, 40-minute in vitro exposures to concentrations of ozone that did not result in any detectable decrease in colony-forming efficiency.

Thomassen, D.G.; Harkema, J.R.; Sun, J.D.; Stephens, N.D.; Griffith, W.C. (Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (United States))

1992-04-01T23:59:59.000Z

117

Myosin VI is required for structural integrity of the apical surface of sensory hair cells in zebrafish  

E-Print Network [OSTI]

Myosin VI is required for structural integrity of the apical surface of sensory hair cells deafness in humans and deafness in Snell's waltzer mice associated with abnormal fusion of hair cell and epithelial morphogenesis, the role of this protein in the sensory hair cells remains unclear. To investigate

Avraham, Karen

118

Cell Prolif. 2007, 40, 106124 2007 The Authors  

E-Print Network [OSTI]

, Madison 53706. Tel.: +608 265 5182; E-mail: Alexander@oncology.wisc.edu #12;Simulating mouse mammary gland

Beebe, David J.

119

Liver progenitor cells develop cholangiocyte-type epithelial polarity in three-dimensional culture.  

E-Print Network [OSTI]

143, 1101–1112. Strick-Marchand, H. , and Weiss, M. C. (et al. , 1998; Strick-Marchand and Weiss, 2002; Ader et

Tanimizu, Naoki; Miyajima, Atsushi; Mostov, Keith E

2007-01-01T23:59:59.000Z

120

Characterizing ATP-dependent activation of inflammasome in gingival epithelial cells  

E-Print Network [OSTI]

26796. Yilmaz, O. , et al. , ATP-dependent activation of anYilmaz, O. , et al. , ATP scavenging by the intracellular1beta release by the ATP-gated P2X7 receptor. EMBO J. ,

Koo, Evonne

2010-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Differences in the regulation of Thrombospondin-1 expression between epithelial cells and fibroblasts  

E-Print Network [OSTI]

Induction of angiogenesis is a critical and rate-limiting step in the progression of cancer. It is widely acknowledged that this induction requires the concomitant stimulation of pro-angiogenic and repression of anti-angiogenic ...

Rodriguez, Roberto Karlo

2007-01-01T23:59:59.000Z

122

tion. Human or mouse intestinal epithelial cells that express the poly-Ig receptor were  

E-Print Network [OSTI]

. Antibodies specific for Clostrid- ium difficile toxin A and Helicobacter pylori urease have been generated, Glauser M et al (1995) Oral immunization with Helicobacter pylori urease as a treatment against Helicobacter infection. Gas- troenterology (in press) Haneberg B, Kendall D, Amerongen HM, Apter FM

Boyer, Edmond

123

3D culture models of normal and malignant breast epithelial cells  

E-Print Network [OSTI]

3D culture models of normal and malignant breast epithelialcells; Lee et al. 3D culture models of normal and malignantFor correspondence: mjbissell@lbl.gov 3D culture models of

Lee, Genee Y.; Kenny, Paraic A.; Lee, Eva H.; Bissell, Mina J.

2006-01-01T23:59:59.000Z

124

Epithelial-derived TGF-2 modulates basal and wound-healing subepithelial matrix homeostasis  

E-Print Network [OSTI]

conditions. We utilized an in vitro model of the epithelial-mesenchymal trophic unit in the human airways increases two- to threefold following scrape injury in a dose-depen- dent fashion and significantly enhances to epithe- lial injury. In the absence of the epithelium, exogenous active TGF- 2 (0­400 pg/ml) produces

George, Steven C.

125

The FASEB Journal Research Communication Calmodulin-dependent activation of the epithelial  

E-Print Network [OSTI]

calcium-dependent chloride channel TMEM16A Yuemin Tian,* Patthara Kongsuphol,* Martin Hug, Jiraporn-dependent activation of the epithelial calcium-dependent chloride channel TMEM16A. FASEB J. 25, 1058­1068 (2011). www 1­10). TMEM16A has 8 putative transmembrane do- mains (TMDs) and a p-loop between TMD 5 and TMD 6

Witzgall, Ralph - Naturwissenschaftliche Fakultät III

126

ABSTRACT Title of Document: ROLE AND REGULATION OF AUTOPHAGY DURING DEVELOPMENTAL CELL DEATH IN DROSOPHILA MELANOGASTER  

E-Print Network [OSTI]

Two prominent morphological forms of programmed cell death occur during development, apoptosis and autophagic cell death. Improper regulation of cell death can lead to a variety of diseases, including cancer. Autophagy is required for survival in response to starvation, but has also been associated with cell death. It is unclear how autophagy is regulated under specific cell contexts in multi-cellular organisms, and what may distinguish autophagy function during cell survival versus cell death. Autophagic cell death is characterized by cells that die in synchrony, with autophagic vacuoles in the cytoplasm, and phagocytosis of the dying cells is not observed. However, little is known about this form of cell death. Autophagic cell death is observed during mammalian development, during regression of the corpus luteum and involution of the mammary and prostate glands. Autophagic cell death is also observed during development of the fruitfly Drosophila melanogaster, during larval salivary gland cell death. Drosophila is an excellent genetic model system to study developmental cell death in vivo. Cells use two main catabolic processes to degrade and recycle cellular

unknown authors

127

Sustained activation of STAT5 is essential for chromatin remodeling and maintenance of mammary-specific function  

E-Print Network [OSTI]

figures Fig S4 relative ?-casein levels Prl (ug/ml)images of Cy5.5-labeled Prl incubated EpH4 cells. EpH4 cellsgrowth factor receptor; Prl, prolactin; lrECM, laminin-rich

Xu, Ren

2010-01-01T23:59:59.000Z

128

Evaluation of Common Angling-Induced Sources of Epithelial Damage for Popular Freshwater Sport Fish using Fluorescein  

SciTech Connect (OSTI)

Angling is a popular recreational activity across the globe and a large proportion of fish captured by anglers are released due to voluntary or mandatory catch-and-release practices. The handling associated with hook removal and return of the fish to their environment can cause physical damage to the epidermal layer of the fish which may affect the condition and survival of released fish. This study investigated possible sources of epithelial damage associated with several different handling methods (i.e. landing net types, interactions with different boat floor surfaces, tournament procedures) commonly used in recreational angling for two popular freshwater sport fish species, largemouth bass (Micropterus salmoides) and northern pike (Esox lucius). Epithelial damage was examined using fluorescein, a non-toxic dye, which has been shown to detect latent epithelial damage. Northern pike exhibited extensive epithelial damage after exposure to several of the induced treatments (i.e., interaction with a carpeted surface, knotted nylon net, and line rolling) but relatively little epithelial damage when exposed to others (i.e., knotless rubber nets, smooth boat surfaces, or lip gripping devices). Largemouth bass did not show significant epithelial damage for any of the treatments, with the exception of fish caught in a semi-professional live release tournament. The detection of latent injuries using fluorescein can be an important management tool as it provides visual examples of potential damage that can be caused by different handling methods. Such visualizations can be used to encourage fish friendly angler behaviour and enhance the survival and welfare of released fish. It can also be used to test new products that are intended to or claim to reduce injury to fish that are to be released. Future research should evaluate the relationship between different levels of epithelial damage and mortality across a range of environmental conditions.

Colotelo, Alison HA; Cooke, Steven J.

2011-05-01T23:59:59.000Z

129

Evaluation of alterations in gene expression in MCF-7 cells induced by the agricultural chemicals Enable and Diazinon  

E-Print Network [OSTI]

in gene expression and metabolism (1). Estrogen, a key steroid hormone, plays a vital role in the development and function of the mammary glands, and is active in neoplasia of this tissue. In both normal and cancer cells, estrogen receptor alpha (ER... of acetylcholine esterase in plasma, RBC and Brain (18). Measure levels of diazinon metabolites such as dialkyl phosphates in urine (20). CYP1A2 and 2B6 are also biomarkers of diazinon toxicity since bioactivation of diazinon is CYP specific (21). Assessment...

Mankame, Tanmayi Pradeep

2005-08-29T23:59:59.000Z

130

Comparative Evaluation of Four Presumptive Tests for Blood to Detect Epithelial Injury on Fish  

SciTech Connect (OSTI)

Current methods of fish epithelial injury detection are limited to gross macroscopic examination that has a subjective bias as well as an inability to reliably quantify the degree of injury. Fluorescein, a presumptive test for blood, has been shown to have the capability to detect and quantify fish epithelial injury. However, there are several other presumptive tests for blood (Bluestar*, phenolphthalein, and HemastixH) that may have benefits over the use of fluorescein, particularly for field research on wild fish. This study investigated the capabilities of these four tests to detect and quantify a variety of injuries commonly encountered by fish (abrasion, cuts, fin frays, and punctures) using the freshwater bluegill Lepomis macrochirus as a model. Fluorescein was consistently found to be the most reliable (i.e., detected the highest proportion of true positive results and rarely detected false positive reactions) of the four presumptive tests for blood compared. Further testing was conducted to examine the reliability of fluorescein. By 24 h after an injury was inflicted, the injury was no longer detectable by fluorescein, and when fluorescein was applied to an injured fish, the fluorescein was no longer detectable 3 h after application. In a comparison of two common anaesthetics used in fisheries research, there was no significant difference in the proportion of injury detected when 3- aminobenzoic acid ethyl ester methanesulfate (tricaine) was used compared with a clove oil and ethanol (1:9) solution. In summary, fluorescein was the most reliable presumptive test for blood examined in this study for the detection and quantification of recent (hours) fish epithelial injury.

Colotelo, Alison HA; Smokorowski, Karen; Haxton, Tim; Cooke, Steven J.

2014-06-01T23:59:59.000Z

131

Journal of Mammary Gland Biology and Neoplasia, Vol. 3, No. 3, 1998 Origin and Secretion of Milk Lipids  

E-Print Network [OSTI]

because the assembled lipid droplets are secreted from the cytoplasm enveloped by cellular membranes. In other cells, such as hepatocytes and enterocytes, lipid is secreted by exocytosis from membrane. Two possible mechanisms for lipid secretion have been proposed: an apical mechanism, in which lipid

Mather, Ian

132

Autocrine TGF-beta and stromal cell-derived factor-1 (SDF-1) signaling drives the evolution of tumor-promoting mammary stromal myofibroblasts  

E-Print Network [OSTI]

Much interest is currently focused on the emerging role of tumor-stroma interactions essential for supporting tumor progression. Carcinoma-associated fibroblasts (CAFs), frequently present in the stroma of human breast ...

Kojima, Yasushi

133

Low Dose IR Creates an Oncogenic Microenvironment by Inducing Premature  

SciTech Connect (OSTI)

Introduction Much of the work addressing ionizing radiation-induced cellular response has been carried out mainly with the traditional cell culture technique involving only one cell type, how cellular response to IR is influenced by the tissue microenvironment remains elusive. By use of a three-dimensional (3D) co-culture system to model critical interactions of different cell types with their neighbors and with their environment, we recently showed that low-dose IR-induced extracellular signaling via the tissue environment affects profoundly cellular responses. This proposal aims at determining the response of mammary epithelial cells in a tissue-like setting.

Yuan, Zhi-Min [Harvard School of Public Health

2013-04-28T23:59:59.000Z

134

Abstract. Breast cancer is the most common cancer in women worldwide. Transformation of a normal cell to a malignant one  

E-Print Network [OSTI]

Abstract. Breast cancer is the most common cancer in women worldwide. Transformation of a normal regulators of growth. Biomarkers associated with cancer were examined in human breast epithelial cells transformed by high-LET radiation in the presence of 17Ã?-estradiol. An established cancer model was used

135

Chronic cadmium exposure in vitro induces cancer cell characteristics in human lung cells  

SciTech Connect (OSTI)

Cadmium is a known human lung carcinogen. Here, we attempt to develop an in vitro model of cadmium-induced human lung carcinogenesis by chronically exposing the peripheral lung epithelia cell line, HPL-1D, to a low level of cadmium. Cells were chronically exposed to 5 ?M cadmium, a noncytotoxic level, and monitored for acquired cancer characteristics. By 20 weeks of continuous cadmium exposure, these chronic cadmium treated lung (CCT-LC) cells showed marked increases in secreted MMP-2 activity (3.5-fold), invasion (3.4-fold), and colony formation in soft agar (2-fold). CCT-LC cells were hyperproliferative, grew well in serum-free media, and overexpressed cyclin D1. The CCT-LC cells also showed decreased expression of the tumor suppressor genes p16 and SLC38A3 at the protein levels. Also consistent with an acquired cancer cell phenotype, CCT-LC cells showed increased expression of the oncoproteins K-RAS and N-RAS as well as the epithelial-to-mesenchymal transition marker protein Vimentin. Metallothionein (MT) expression is increased by cadmium, and is typically overexpressed in human lung cancers. The major MT isoforms, MT-1A and MT-2A were elevated in CCT-LC cells. Oxidant adaptive response genes HO-1 and HIF-1A were also activated in CCT-LC cells. Expression of the metal transport genes ZNT-1, ZNT-5, and ZIP-8 increased in CCT-LC cells culminating in reduced cadmium accumulation, suggesting adaptation to the metal. Overall, these data suggest that exposure of human lung epithelial cells to cadmium causes acquisition of cancer cell characteristics. Furthermore, transformation occurs despite the cell's ability to adapt to chronic cadmium exposure. - Highlights: • Chronic cadmium exposure induces cancer cell characteristics in human lung cells. • This provides an in vitro model of cadmium-induced human lung cell transformation. • This occurred with general and lung specific changes typical for cancer cells. • These findings add insight to the relationship between cadmium and lung cancer.

Person, Rachel J.; Tokar, Erik J.; Xu, Yuanyuan; Orihuela, Ruben; Ngalame, Ntube N. Olive; Waalkes, Michael P., E-mail: waalkes@niehs.nih.gov

2013-12-01T23:59:59.000Z

136

Force localization in contracting cell layers  

E-Print Network [OSTI]

Epithelial cell layers on soft elastic substrates or pillar arrays are commonly used as model systems for investigating the role of force in tissue growth, maintenance and repair. Here we show analytically that the experimentally observed localization of traction forces to the periphery of the cell layers does not necessarily imply increased local cell activity, but follows naturally from the elastic problem of a finite-sized contractile layer coupled to an elastic foundation. For homogeneous contractility, the force localization is determined by one dimensionless parameter interpolating between linear and exponential force profiles for the extreme cases of very soft and very stiff substrates, respectively. If contractility is sufficiently increased at the periphery, outward directed displacements can occur at intermediate positions, although the edge itself still retracts. We also show that anisotropic extracellular stiffness leads to force localization in the stiffer direction, as observed experimentally.

Carina M. Edwards; Ulrich S. Schwarz

2012-01-13T23:59:59.000Z

137

Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system  

E-Print Network [OSTI]

for Biotechnology of the Republic of Kazakhstan, Astana 010000, Kazakhstan a r t i c l e i n f o Article history

Revzin, Alexander

138

Purinergic receptors responsible for ATP-dependent ROS production and activation of inflammasomes in gingival epithelial cells  

E-Print Network [OSTI]

D. S. Kumararatne. 1997. ATP-induced killing of mycobacteria24. Cruz, C.M. , et al. , ATP activates a reactive oxygenYilmaz, O. , et al. , ATP scavenging by the intracellular

Ho, Marcus

2012-01-01T23:59:59.000Z

139

Arp2/3 Inhibition Induces Amoeboid-Like Protrusions in MCF10A Epithelial Cells by Reduced Cytoskeletal-  

E-Print Network [OSTI]

Stricker3,4 , Kareem Sayegh3,4 , Clement Campillo5,6 , Margaret L. Gardel1,3,4 * 1 Institute required for its stabilization. Citation: Beckham Y, Vasquez RJ, Stricker J, Sayegh K, Campillo C, et al

Gardel, Margaret

140

Purinergic receptors responsible for ATP-dependent ROS production and activation of inflammasomes in gingival epithelial cells  

E-Print Network [OSTI]

and G. Dahl, Probenecid, a gout remedy, inhibits pannexin-1Martinon F et al. (2006) Gout-associated uric acid crystalsincluding type I diabetes, gout, and many autoinflammatory

Ho, Marcus

2012-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

TGFb-Dependent Epithelial-to-Mesenchymal Transition Is Required to Generate Cardiospheres  

E-Print Network [OSTI]

and CSp-derived cells grown in a monolayer. EMT and CSps formation is enhanced in the presence of primary cardiac explants (ex- plant-derived cells [EDCs]) and form cardiospheres (CSps) which recreate in vitro a niche-like microtissue [5]. CSp-de- rived cells (CDCs) can be expanded in monolayers [6

Paris-Sud XI, Université de

142

Evaluation of Yeast Cell Wall on Early Production Laying Hen Performance  

E-Print Network [OSTI]

from non-hydrolyzable oligo and polysaccharides increases the proliferation of the gut epithelial cells, thus increasing intestinal tissue weight, with changes in the overall morphology of intestinal mucosa (Niba et al., 2009; Bonos et al., 2011... and multiply within the digestive tract mucosa (Baurhoo et al., 2007b; Bonos et al., 2011; Jacobs, 2011). Types of prebiotics Most identified prebiotics are classified as carbohydrate oligosaccharides with differing molecular structure that are normally...

Hashim, Mohammed Malik Hashim 1981-

2012-11-08T23:59:59.000Z

143

Regulation of epithelial-mesenchymal transition and DNA damage responses by singleminded-2s  

E-Print Network [OSTI]

and progression in breast cancer, we depleted SIM2 RNA in MCF-7 cells using a retroviral shRNA system and examined gene expression and functional abilities of the SIM2-depleted MCF-7 cells (SIM2i) relative to a control MCF line expressing a non-specific “scrambled...

Laffin, Brian Edward

2009-05-15T23:59:59.000Z

144

Differential effects on cell motility, embryonic stem cell self-renewal and senescence by diverse Src kinase family inhibitors  

SciTech Connect (OSTI)

The Src family of non-receptor tyrosine kinases (SFKs) has been shown to play an intricate role in embryonic stem (ES) cell maintenance. In the present study we have focused on the underlying molecular mechanisms responsible for the vastly different effects induced by various commonly used SFK inhibitors. We show that several diverse cell types, including fibroblasts completely lacking SFKs, cannot undergo mitosis in response to SU6656 and that this is caused by an unselective inhibition of Aurora kinases. In contrast, PP2 and PD173952 block motility immediately upon exposure and forces cells to grow in dense colonies. The subsequent halt in proliferation of fibroblast and epithelial cells in the center of the colonies approximately 24 h post-treatment appears to be caused by cell-to-cell contact inhibition rather than a direct effect of SFK kinase inhibition. Interestingly, in addition to generating more homogenous and dense ES cell cultures, without any diverse effect on proliferation, PP2 and PD173652 also promote ES cell self-renewal by reducing the small amount of spontaneous differentiation typically observed under standard ES cell culture conditions. These effects could not be mirrored by the use of Gleevec, a potent inhibitor of c-Abl and PDGFR kinases that are also inhibited by PP2. -- Highlights: Black-Right-Pointing-Pointer SFK inhibitor SU6656 induces senescence in mouse ES cells. Black-Right-Pointing-Pointer SU6656 inhibits mitosis in a SFK-independent manner via cross-selectivity for Aurora kinases. Black-Right-Pointing-Pointer SFK inhibitor PP2 impairs cell motility in various cell lines, including mouse ES cells. Black-Right-Pointing-Pointer Ensuing impeded motility, PP2 inhibits proliferation of various cells lines except for mouse ES cells. Black-Right-Pointing-Pointer SFK inhibitors PP2 and PD173952 impede spontaneous differentiation in standard mouse ES culture maintenance.

Tamm, Christoffer, E-mail: christoffer.tamm@imbim.uu.se; Galito, Sara Pijuan, E-mail: sara.pijuan@imbim.uu.se; Anneren, Cecilia, E-mail: cecilia.anneren@imbim.uu.se

2012-02-15T23:59:59.000Z

145

Id-1 and Id-2 genes and products as markers of epithelial cancer  

DOE Patents [OSTI]

A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

Desprez, Pierre-Yves (El Cerrito, CA); Campisi, Judith (Berkeley, CA)

2011-10-04T23:59:59.000Z

146

Id-1 and Id-2 genes and products as markers of epithelial cancer  

DOE Patents [OSTI]

A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is a prognostic indicator that breast cancer cells are invasive and metastatic, whereas Id-2 gene is a prognostic indicator that breast cancer cells are localized and noninvasive in the breast tissue.

Desprez, Pierre-Yves (El Cerrito, CA); Campisi, Judith (Berkeley, CA)

2008-09-30T23:59:59.000Z

147

Digitoxin and a synthetic monosaccharide analog inhibit cell viability in lung cancer cells  

SciTech Connect (OSTI)

Mechanisms of digitoxin-inhibited cell growth and induced apoptosis in human non-small cell lung cancer (NCI-H460) cells remain unclear. Understanding how digitoxin or derivate analogs induce their cytotoxic effect below therapeutically relevant concentrations will help in designing and developing novel, safer and more effective anti-cancer drugs. In this study, NCI-H460 cells were treated with digitoxin and a synthetic analog D6-MA to determine their anti-cancer activity. Different concentrations of digitoxin and D6-MA were used and the subsequent changes in cell morphology, viability, cell cycle, and protein expressions were determined. Digitoxin and D6-MA induced dose-dependent apoptotic morphologic changes in NCI-H460 cells via caspase-9 cleavage, with D6-MA possessing 5-fold greater potency than digitoxin. In comparison, non-tumorigenic immortalized bronchial and small airway epithelial cells displayed significantly less apoptotic sensitivity compared to NCI-H460 cells suggesting that both digitoxin and D6-MA were selective for NSCLC. Furthermore, NCI-H460 cells arrested in G(2)/M phase following digitoxin and D6-MA treatment. Post-treatment evaluation of key G2/M checkpoint regulatory proteins identified down-regulation of cyclin B1/cdc2 complex and survivin. Additionally, Chk1/2 and p53 related proteins experienced down-regulation suggesting a p53-independent cell cycle arrest mechanism. In summary, digitoxin and D6-MA exert anti-cancer effects on NCI-H460 cells through apoptosis or cell cycle arrest, with D6-MA showing at least 5-fold greater potency relative to digitoxin. -- Highlights: ? Digitoxin and synthetic analog D6-MA induced apoptotic morphologic changes in NCI-H460 cells in a dose-dependent manner. ? Apoptotic cell death induced by analog was 5-fold more potent when compared to digitoxin. ? NCI-H460 cells arrested in G(2)/M phase following digitoxin (? 5 nM) and analog (? 1 nM) treatment. ? Digitoxin inhibited the expression of cyclin B1/cdc2 complex and survivin at sub-therapeutic concentrations. ? D6-MA was 4-fold more potent than digitoxin.

Elbaz, Hosam A. [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Stueckle, Todd A. [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States) [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); National Institute for Occupational Safety and Health, Morgantown, WV26506 (United States); Wang, Hua-Yu Leo; O'Doherty, George A. [Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115 (United States)] [Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115 (United States); Lowry, David T.; Sargent, Linda M.; Wang, Liying [National Institute for Occupational Safety and Health, Morgantown, WV26506 (United States)] [National Institute for Occupational Safety and Health, Morgantown, WV26506 (United States); Dinu, Cerasela Zoica, E-mail: cerasela-zoica.dinu@mail.wvu.edu [Department of Chemical Engineering, West Virginia University, Morgantown, WV 26506 (United States); Rojanasakul, Yon, E-mail: yrojan@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)

2012-01-01T23:59:59.000Z

148

Context dependent reversion of tumor phenotype by connexin-43 expression in MDA-MB231 cells and MCF-7 cells: Role of ?-catenin/connexin43 association  

SciTech Connect (OSTI)

Connexins (Cx), gap junction (GJ) proteins, are regarded as tumor suppressors, and Cx43 expression is often down regulated in breast tumors. We assessed the effect of Cx43 over-expression in 2D and 3D cultures of two breast adenocarcinoma cell lines: MCF-7 and MDA-MB-231. While Cx43 over-expression decreased proliferation of 2D and 3D cultures of MCF-7 by 56% and 80% respectively, MDA-MB-231 growth was not altered in 2D cultures, but exhibited 35% reduction in 3D cultures. C-terminus truncated Cx43 did not alter proliferation. Untransfected MCF-7 cells formed spherical aggregates in 3D cultures, and MDA-MB-231 cells formed stellar aggregates. However, MCF-7 cells over-expressing Cx43 formed smaller sized clusters and Cx43 expressing MDA-MB-231 cells lost their stellar morphology. Extravasation ability of both MCF-7 and MDA-MB-231 cells was reduced by 60% and 30% respectively. On the other hand, silencing Cx43 in MCF10A cells, nonneoplastic human mammary cell line, increased proliferation in both 2D and 3D cultures, and disrupted acinar morphology. Although Cx43 over-expression did not affect total levels of ?-catenin, ?-catenin and ZO-2, it decreased nuclear levels of ?-catenin in 2D and 3D cultures of MCF-7 cells, and in 3D cultures of MDA-MB-231 cells. Cx43 associated at the membrane with ?-catenin, ?-catenin and ZO-2 in 2D and 3D cultures of MCF-7 cells, and only in 3D conditions in MDA-MB-231 cells. This study suggests that Cx43 exerts tumor suppressive effects in a context-dependent manner where GJ assembly with ?-catenin, ?-catenin and ZO-2 may be implicated in reducing growth rate, invasiveness, and, malignant phenotype of 2D and 3D cultures of MCF-7 cells, and 3D cultures of MDA-MB-231 cells, by sequestering ?-catenin away from nucleus. - Highlights: • Cx43 over-expressing MCF-7 and MDA-MB-231 were grown in 2D and 3D cultures. • Proliferation and growth morphology were affected in a context dependent manner. • Extravasation ability of both MCF-7 and MDA-MB-231 cells was reduced. • Cx43-mediated gap junction complex assembly correlated with observed changes. • We propose that membranous Cx43 sequesters ?-catenin away from the nucleus.

Talhouk, Rabih S., E-mail: rtalhouk@aub.edu.lb [Department of Biology, Faculty of Arts and Sciences, American University of Beirut, P.O. Box 11-0236, Beirut (Lebanon); Fares, Mohamed-Bilal; Rahme, Gilbert J.; Hariri, Hanaa H.; Rayess, Tina; Dbouk, Hashem A.; Bazzoun, Dana; Al-Labban, Dania [Department of Biology, Faculty of Arts and Sciences, American University of Beirut, P.O. Box 11-0236, Beirut (Lebanon); El-Sabban, Marwan E., E-mail: me00@aub.edu.lb [Department of Anatomy, Cell Biology and Physiology, Faculty of Medicine, American University of Beirut, P.O. Box 11-0236, Beirut (Lebanon)

2013-12-10T23:59:59.000Z

149

NON-INVASIVE OPTICAL DETECTION OF EPITHELIAL CANCER USING OBLIQUE INCIDENCE DIFFUSE REFLECTANCE SPECTROSCOPY  

E-Print Network [OSTI]

-pigmented lesions...................... 50 25 Results of the testing dataset for the non-pigmented lesions ..................... 51 26 Absorption coefficient spectra of common nevi, dysplastic nevi and melanoma... of abnormal cells that can only be seen by 5 histologic analysis and not through the endoscope. The current recommendation for patients who have a stable diagnosis of negative for dysplasia, confirmed by two endoscopic biopsy surveillance procedures...

Garcia-Uribe, Alejandro

2010-01-16T23:59:59.000Z

150

Calcitriol inhibits Ether-a go-go potassium channel expression and cell proliferation in human breast cancer cells  

SciTech Connect (OSTI)

Antiproliferative actions of calcitriol have been shown to occur in many cell types; however, little is known regarding the molecular basis of this process in breast carcinoma. Ether-a-go-go (Eag1) potassium channels promote oncogenesis and are implicated in breast cancer cell proliferation. Since calcitriol displays antineoplastic effects while Eag1 promotes tumorigenesis, and both factors antagonically regulate cell cycle progression, we investigated a possible regulatory effect of calcitriol upon Eag1 as a mean to uncover new molecular events involved in the antiproliferative activity of this hormone in human breast tumor-derived cells. RT real-time PCR and immunocytochemistry showed that calcitriol suppressed Eag1 expression by a vitamin D receptor (VDR)-dependent mechanism. This effect was accompanied by inhibition of cell proliferation, which was potentiated by astemizole, a nonspecific Eag1 inhibitor. Immunohistochemistry and Western blot demonstrated that Eag1 and VDR abundance was higher in invasive-ductal carcinoma than in fibroadenoma, and immunoreactivity of both proteins was located in ductal epithelial cells. Our results provide evidence of a novel mechanism involved in the antiproliferative effects of calcitriol and highlight VDR as a cancer therapeutic target for breast cancer treatment and prevention.

Garcia-Becerra, Rocio [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Diaz, Lorenza, E-mail: lorenzadiaz@gmail.com [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Camacho, Javier [Department of Pharmacology, Centro de Investigacion y de Estudios Avanzados, Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco 07360, Mexico, D.F. (Mexico)] [Department of Pharmacology, Centro de Investigacion y de Estudios Avanzados, Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco 07360, Mexico, D.F. (Mexico); Barrera, David; Ordaz-Rosado, David; Morales, Angelica [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Ortiz, Cindy Sharon [Department of Pathology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Pathology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Avila, Euclides [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico); Bargallo, Enrique [Department of Breast Tumors, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico)] [Department of Breast Tumors, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico); Arrecillas, Myrna [Department of Pathology, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico)] [Department of Pathology, Instituto Nacional de Cancerologia, Av. San Fernando No. 22, Tlalpan 14080, Mexico, D.F. (Mexico); Halhali, Ali; Larrea, Fernando [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)] [Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No. 15, Tlalpan 14000 Mexico, D.F. (Mexico)

2010-02-01T23:59:59.000Z

151

Wnt interaction and extracellular release of prominin-1/CD133 in human malignant melanoma cells  

SciTech Connect (OSTI)

Prominin-1 (CD133) is the first identified gene of a novel class of pentaspan membrane glycoproteins. It is expressed by various epithelial and non-epithelial cells, and notably by stem and cancer stem cells. In non-cancerous cells such as neuro-epithelial and hematopoietic stem cells, prominin-1 is selectively concentrated in plasma membrane protrusions, and released into the extracellular milieu in association with small vesicles. Previously, we demonstrated that prominin-1 contributes to melanoma cells pro-metastatic properties and suggested that it may constitute a molecular target to prevent prominin-1-expressing melanomas from colonizing and growing in lymph nodes and distant organs. Here, we report that three distinct pools of prominin-1 co-exist in cultures of human FEMX-I metastatic melanoma. Morphologically, in addition to the plasma membrane localization, prominin-1 is found within the intracellular compartments, (e.g., Golgi apparatus) and in association with extracellular membrane vesicles. The latter prominin-1–positive structures appeared in three sizes (small, ?40 nm; intermediates ?40–80 nm, and large, >80 nm). Functionally, the down-regulation of prominin-1 in FEMX-I cells resulted in a significant reduction of number of lipid droplets as observed by coherent anti-Stokes Raman scattering image analysis and Oil red O staining, and surprisingly in a decrease in the nuclear localization of beta-catenin, a surrogate marker of Wnt activation. Moreover, the T-cell factor/lymphoid enhancer factor (TCF/LEF) promoter activity was 2 to 4 times higher in parental than in prominin-1-knockdown cells. Collectively, our results point to Wnt signaling and/or release of prominin-1–containing membrane vesicles as mediators of the pro-metastatic activity of prominin-1 in FEMX-I melanoma. - Highlights: ? First report of release of prominin-1–containing microvesicles from cancer cells. ? Pro-metastatic role of prominin-1–containing microvesicles in FEMX-I melanoma. ? Down-regulation of prominin-1 results in decreased nuclear localization of ?-catenin. ? Wnt signaling as mediator of the pro-metastatic activity of prominin-1.

Rappa, Germana [Cancer Research Program, Roseman University of Health Sciences, 10530 Discovery Drive. Las Vegas, NV 89135 (United States); College of Pharmacy, Roseman University of Health Sciences, Henderson, NV 89104 (United States); Mercapide, Javier; Anzanello, Fabio [Cancer Research Program, Roseman University of Health Sciences, 10530 Discovery Drive. Las Vegas, NV 89135 (United States); Le, Thuc T. [Nevada Cancer Institute, Las Vegas, NV 89135 (United States); Johlfs, Mary G. [Cancer Research Program, Roseman University of Health Sciences, 10530 Discovery Drive. Las Vegas, NV 89135 (United States); Center for Diabetes and Obesity Prevention, Treatment, Research and Education, Roseman University of Health Sciences, Henderson, NV 89104 (United States); Fiscus, Ronald R. [Cancer Research Program, Roseman University of Health Sciences, 10530 Discovery Drive. Las Vegas, NV 89135 (United States); College of Pharmacy, Roseman University of Health Sciences, Henderson, NV 89104 (United States); Center for Diabetes and Obesity Prevention, Treatment, Research and Education, Roseman University of Health Sciences, Henderson, NV 89104 (United States); Wilsch-Bräuninger, Michaela [Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden (Germany); Corbeil, Denis [Tissue Engineering Laboratories (BIOTEC) and DFG Research Center and Cluster of Excellence for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Tatzberg 47–49, 01307 Dresden, Germany Technische Universitat Dresden, Dresden (Germany); Lorico, Aurelio, E-mail: alorico@roseman.edu [Cancer Research Program, Roseman University of Health Sciences, 10530 Discovery Drive. Las Vegas, NV 89135 (United States); College of Pharmacy, Roseman University of Health Sciences, Henderson, NV 89104 (United States)

2013-04-01T23:59:59.000Z

152

Electrochemical cell  

DOE Patents [OSTI]

An electrochemical cell is described having a bimodal positive electrode, a negative electrode of an alkali metal, and a compatible electrolyte including an alkali metal salt molten at the cell operating temperature. The positive electrode has an electrochemically active layer of at least one transition metal chloride at least partially present as a charging product, and additives of bromide and/or iodide and sulfur in the positive electrode or the electrolyte. Electrode volumetric capacity is in excess of 400 Ah/cm[sup 3]; the cell can be 90% recharged in three hours and can operate at temperatures below 160 C. There is also disclosed a method of reducing the operating temperature and improving the overall volumetric capacity of an electrochemical cell and for producing a positive electrode having a BET area greater than 6[times]10[sup 4] cm[sup 2]/g of Ni. 8 figures.

Redey, L.I.; Vissers, D.R.; Prakash, J.

1994-02-01T23:59:59.000Z

153

E-Print Network 3.0 - acid inhibits tumor Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

we show here that intrametastatic lymphatic vessels and bulk tumor cell... model of tumor bulk invasion, human mammary carcinoma cells caused ... Source: Floreano, Dario -...

154

Ectopic ERK Expression Induces Phenotypic Conversion of C10 Cells and Alters DNA Methyltransferase Expression  

SciTech Connect (OSTI)

In some model systems constitutive extracellular signal regulated kinase (ERK) activation is sufficient to promote an oncogenic phenotype. Here we investigate whether constitutive ERK expression influences phenotypic conversion in murine C10 type II alveolar epithelial cells. C10 cells were stably transduced with an ERK1-green fluorescent protein (ERK1-GFP) chimera or empty vector and ectopic ERK expression was associated with the acquisition of soft agar focus-forming potential in late passage, but not early passage cells. Late passage ERK1-GFP cells exhibited a significant increase in the expression of DNA methyl transferases (DNMT1 and 3b) and a marked increase in sensitivity to 5-azacytidine (5-azaC)-mediated toxicity, relative to early passage ERK1-GFP cells and vector controls. The expression of xeroderma pigmentosum complementation group A (XPA) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) were significantly increased in late passage cells, suggesting enhanced DNA damage recognition and repair activity which we interpret as a reflection of genomic instability. Phospho-ERK levels were dramatically decreased in late passage ERK1-GFP cells, relative to early passage and vector controls, and phospho-ERK levels were restored by treatment with sodium orthovanadate, indicating a role for phosphatase activity in this response. Collectively these observations suggest that ectopic ERK expression promotes phenotypic conversion of C10 cells that is associated with latent effects on epigenetic programming and phosphatase activities.

Sontag, Ryan L.; Weber, Thomas J.

2012-05-04T23:59:59.000Z

155

Transmembrane protein PERP is a component of tessellate junctions and of other junctional and non-junctional plasma membrane regions in diverse epithelial and epithelium-derived cells  

E-Print Network [OSTI]

Protein PERP (p53 apoptosis effector related to PMP-22) is a small (21.4 kDa) transmembrane polypeptide with an amino acid sequence indicative of a tetraspanin character. It is enriched in the plasma membrane and apparently ...

Franke, Werner W.

156

Inhibiting Vimentin or beta 1-integrin Reverts Prostate Tumor Cells in IrECM and Reduces Tumor Growth  

SciTech Connect (OSTI)

Prostate epithelial cells grown embedded in laminin-rich extracellular matrix (lrECM) undergo morphological changes that closely resemble their architecture in vivo. In this study, growth characteristics of three human prostate epithelial sublines derived from the same cellular lineage, but displaying different tumorigenic and metastatic properties in vivo, were assessed in three-dimensional (3D) lrECM gels. M12, a highly tumorigenic and metastatic subline, was derived from the parental prostate epithelial P69 cell line by selection in nude mice and found to contain a deletion of 19p-q13.1. The stable reintroduction of an intact human chromosome 19 into M12 resulted in a poorly tumorigenic subline, designated F6. When embedded in lrECM gels, the nontumorigenic P69 line produced acini with clearly defined lumena. Immunostaining with antibodies to {beta}-catenin, E-cadherin or {alpha}6-, {beta}4- and {beta}1-integrins showed polarization typical of glandular epithelium. In contrast, the metastatic M12 subline produced highly disorganized cells with no evidence of polarization. The F6 subline reverted to acini-like structures exhibiting basal polarity marked with integrins. Reducing either vimentin levels via siRNA interference or {beta}1-integrin expression by the addition of the blocking antibody, AIIB2, reorganized the M12 subline into forming polarized acini. The loss of vimentin significantly reduced M12-Vim tumor growth when assessed by subcutaneous injection in athymic mice. Thus, tumorigenicity in vivo correlated with disorganized growth in 3D lrECM gels. These studies suggest that the levels of vimentin and {beta}1-integrin play a key role in the homeostasis of the normal acini in prostate and that their dysregulation may lead to tumorigenesis.

Zhang, Xueping; Fournier, Marcia V.; Ware, Joy L.; Bissell, Mina J.; Zehner, Zendra E.

2009-07-27T23:59:59.000Z

157

Electrochemical cell  

DOE Patents [OSTI]

An electrochemical cell is described having an alkali metal negative electrode such as sodium and a positive electrode including Ni or transition metals, separated by a [beta] alumina electrolyte and NaAlCl[sub 4] or other compatible material. Various concentrations of a bromine, iodine and/or sulfur containing additive and pore formers are disclosed, which enhance cell capacity and power. The pore formers may be the ammonium salts of carbonic acid or a weak organic acid or oxamide or methylcellulose. 6 figs.

Redey, L.I.; Vissers, D.R.; Prakash, J.

1994-08-23T23:59:59.000Z

158

Electrochemical cell  

DOE Patents [OSTI]

An improved secondary electrochemical cell is disclosed having a negative electrode of lithium aluminum, a positive electrode of iron sulfide, a molten electrolyte of lithium chloride and potassium chloride, and the combination that the fully charged theoretical capacity of the negative electrode is in the range of 0.5-1.0 that of the positive electrode. The cell thus is negative electrode limiting during discharge cycling. Preferably, the negative electrode contains therein, in the approximate range of 1-10 volume % of the electrode, an additive from the materials of graphitized carbon, aluminum-iron alloy, and/or magnesium oxide.

Kaun, Thomas D. (New Lenox, IL)

1984-01-01T23:59:59.000Z

159

Disruption of canonical TGF?-signaling in murine coronary progenitor cells by low level arsenic  

SciTech Connect (OSTI)

Exposure to arsenic results in several types of cancers as well as heart disease. A major contributor to ischemic heart pathologies is coronary artery disease, however the influences by environmental arsenic in this disease process are not known. Similarly, the impact of toxicants on blood vessel formation and function during development has not been studied. During embryogenesis, the epicardium undergoes proliferation, migration, and differentiation into several cardiac cell types including smooth muscle cells which contribute to the coronary vessels. The TGF? family of ligands and receptors is essential for developmental cardiac epithelial to mesenchymal transition (EMT) and differentiation into coronary smooth muscle cells. In this in vitro study, 18 hour exposure to 1.34 ?M arsenite disrupted developmental EMT programming in murine epicardial cells causing a deficit in cardiac mesenchyme. The expression of EMT genes including TGF?2, TGF? receptor-3, Snail, and Has-2 are decreased in a dose-dependent manner following exposure to arsenite. TGF?2 cell signaling is abrogated as detected by decreases in phosphorylated Smad2/3 when cells are exposed to 1.34 ?M arsenite. There is also loss of nuclear accumulation pSmad due to arsenite exposure. These observations coincide with a decrease in vimentin positive mesenchymal cells invading three-dimensional collagen gels. However, arsenite does not block TGF?2 mediated smooth muscle cell differentiation by epicardial cells. Overall these results show that arsenic exposure blocks developmental EMT gene programming in murine coronary progenitor cells by disrupting TGF?2 signals and Smad activation, and that smooth muscle cell differentiation is refractory to this arsenic toxicity. - Highlights: • Arsenic blocks TGF?2 induced expression of EMT genes. • Arsenic blocks TGF?2 triggered Smad2/3 phosphorylation and nuclear translocation. • Arsenic blocks epicardial cell differentiation into cardiac mesenchyme. • Arsenic does not block TGF?2 induced smooth muscle cell differentiation.

Allison, Patrick; Huang, Tianfang; Broka, Derrick; Parker, Patti [Department of Pharmacology and Toxicology College of Pharmacy, Southwest Environmental Health Sciences Center, Steele Children's Research Center and Bio5 Institute, University of Arizona, Tucson, AZ 85721 (United States); Barnett, Joey V. [Department of Pharmacology, Vanderbilt Medical University, Nashville, TN (United States); Camenisch, Todd D., E-mail: camenisch@pharmacy.arizona.edu [Department of Pharmacology and Toxicology College of Pharmacy, Southwest Environmental Health Sciences Center, Steele Children's Research Center and Bio5 Institute, University of Arizona, Tucson, AZ 85721 (United States)

2013-10-01T23:59:59.000Z

160

Photovoltaic cell  

DOE Patents [OSTI]

In a photovoltaic cell structure containing a visibly transparent, electrically conductive first layer of metal oxide, and a light-absorbing semiconductive photovoltaic second layer, the improvement comprising a thin layer of transition metal nitride, carbide or boride interposed between said first and second layers.

Gordon, Roy G. (Cambridge, MA); Kurtz, Sarah (Somerville, MA)

1984-11-27T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Nanocrystal Solar Cells  

E-Print Network [OSTI]

research on organic photovoltaic cells since small molecule10 years prior (4). Photovoltaic cells with an active layerof the associated photovoltaic cells. 2.4 Charge transport

Gur, Ilan

2006-01-01T23:59:59.000Z

162

Nanocrystal Solar Cells  

E-Print Network [OSTI]

Nov, 2005). Chapter 4 Hybrid solar cells with 3-dimensionalinorganic nanocrystal solar cells 5.1 Introduction In recentoperation of organic based solar cells and distinguish them

Gur, Ilan

2006-01-01T23:59:59.000Z

163

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

Research Institute 1990 Fuel Cell Status," Proceedings ofMiller, "Introduction: Fuel-Cell-Powered Vehicle DevelopmentPrograms," presented at Fuel Cells for Transportation,

Delucchi, Mark

1992-01-01T23:59:59.000Z

164

Diagnostic Studies on Lithium Battery Cells and Cell Components...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Studies on Lithium Battery Cells and Cell Components Diagnostic Studies on Lithium Battery Cells and Cell Components 2012 DOE Hydrogen and Fuel Cells Program and Vehicle...

165

Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar | Department...  

Broader source: Energy.gov (indexed) [DOE]

Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar Fuel Cell Technologies Overview: 2011 Fuel Cell Seminar Presentation by Sunita Satyapal at the Fuel Cell Seminar on November...

166

Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities Stationary Fuel Cells: Overview of Hydrogen and Fuel Cell Activities Presentation covers stationary fuel cells...

167

Electrochemistry Cell Model  

Broader source: Energy.gov (indexed) [DOE]

or exceeds all performance goals - Interpreting complex cell electrochemical phenomena - Identification of cell degradation mechanisms Partners (Collaborators) Daniel Abraham,...

168

Telecommunications International Cell Phone  

E-Print Network [OSTI]

Telecommunications International Cell Phone 1. Fax completed form to 979.847.1111. 2. If you do will be charged. Date Cell Phone Needed Cell Phone Pick-Up Date Cell Phone User Travel Destination(s) United States Number Destination Country Number Cell Phone Type Digital Satellite Cell Phone Return Date Notes

169

Knockdown of dual specificity phosphatase 4 enhances the chemosensitivity of MCF-7 and MCF-7/ADR breast cancer cells to doxorubicin  

SciTech Connect (OSTI)

Background: Breast cancer is the major cause of cancer-related deaths in females world-wide. Doxorubicin-based therapy has limited efficacy in breast cancer due to drug resistance, which has been shown to be associated with the epithelial-to-mesenchymal transition (EMT). However, the molecular mechanisms linking the EMT and drug resistance in breast cancer cells remain unclear. Dual specificity phosphatase 4 (DUSP4), a member of the dual specificity phosphatase family, is associated with cellular proliferation and differentiation; however, its role in breast cancer progression is controversial. Methods: We used cell viability assays, Western blotting and immunofluorescent staining, combined with siRNA interference, to evaluate chemoresistance and the EMT in MCF-7 and adriamycin-resistant MCF-7/ADR breast cancer cells, and investigate the underlying mechanisms. Results: Knockdown of DUSP4 significantly increased the chemosensitivity of MCF-7 and MCF-7/ADR breast cancer cells to doxorubicin, and MCF-7/ADR cells which expressed high levels of DUSP4 had a mesenchymal phenotype. Furthermore, knockdown of DUSP4 reversed the EMT in MCF-7/ADR cells, as demonstrated by upregulation of epithelial biomarkers and downregulation of mesenchymal biomarkers, and also increased the chemosensitivity of MCF-7/ADR cells to doxorubicin. Conclusions: DUSP4 might represent a potential drug target for inhibiting drug resistance and regulating the process of the EMT during the treatment of breast cancer. - Highlights: • We used different technologies to prove our conclusion. • DUSP4 knockdown increased doxorubicin chemosensitivity in breast cancer cells. • DUSP4 is a potential target for combating drug resistance in breast cancer. • DUSP4 is a potential target for regulating the EMT in breast cancer.

Liu, Yu; Du, Feiya; Chen, Wei; Yao, Minya; Lv, Kezhen; Fu, Peifen, E-mail: fupeifendoczju@163.com

2013-12-10T23:59:59.000Z

170

Three Human Cell Types Respond to Multi-Walled Carbon Nanotubes and Titanium Dioxide Nanobelts with Cell-Specific Transcriptomic and Proteomic Expression Patterns.  

SciTech Connect (OSTI)

The growing use of engineered nanoparticles (NPs) in commercial and medical applications raises the urgent need for tools that can predict NP toxicity. Global transcriptome and proteome analyses were conducted on three human cell types, exposed to two high aspect ratio NP types, to identify patterns of expression that might indicate high versus low NP toxicity. Three cell types representing the most common routes of human exposure to NPs, including macrophage-like (THP-1), small airway epithelial and intestinal (Caco-2/HT29-MTX) cells, were exposed to TiO2 nanobelts (TiO2-NB; high toxicity) and multi-walled carbon nanotubes (MWCNT; low toxicity) at low (10 µg/mL) and high (100 µg/mL) concentrations for 1 and 24 h. Unique patterns of gene and protein expressions were identified for each cell type, with no differentially expressed (p < 0.05, 1.5-fold change) genes or proteins overlapping across all three cell types. While unique to each cell type, the early response was primarily independent of NP type, showing similar expression patterns in response to both TiO2-NB and MWCNT. The early response might, therefore, indicate a general response to insult. In contrast, the 24 h response was unique to each NP type. The most significantly (p < 0.05) enriched biological processes in THP-1 cells indicated TiO2-NB regulation of pathways associated with inflammation, apoptosis, cell cycle arrest, DNA replication stress and genomic instability, while MWCNT-regulated pathways indicated increased cell proliferation, DNA repair and anti-apoptosis. These two distinct sets of biological pathways might, therefore, underlie cellular responses to high and low NP toxicity, respectively.

Tilton, Susan C.; Karin, Norman J.; Tolic, Ana; Xie, Yumei; Lai, Xianyin; Hamilton, Raymond F.; Waters, Katrina M.; Holian, Andrij; Witzmann, Frank A.; Orr, Galya

2014-08-01T23:59:59.000Z

171

Photoelectrochemical cell  

DOE Patents [OSTI]

A photoelectrochemical cell comprising a sealed container having a light-transmitting window for admitting light into the container across a light-admitting plane, an electrolyte in the container, a photoelectrode in the container having a light-absorbing surface arranged to receive light from the window and in contact with the electrolyte, the surface having a plurality of spaced portions oblique to the plane, each portion having dimensions at least an order of magnitude larger than the maximum wavelength of incident sunlight, the total surface area of the surface being larger than the area of the plane bounded by the container, and a counter electrode in the container in contact with the electrolyte.

Rauh, R. David (Newton, MA); Boudreau, Robert A. (Norton, MA)

1983-06-14T23:59:59.000Z

172

Disturbance of DKK1 level is partly involved in survival of lung cancer cells via regulation of ROMO1 and ?-radiation sensitivity  

SciTech Connect (OSTI)

Highlights: •DKK1 was expressed differently among non-small-cell lung cancer cell lines. •DKK1 negatively regulated ROMO1 gene expression. •Disturbance of DKK1 level induced the imbalance of cellular ROS. •DKK1/ROMO1-induced ROS imbalance is involved in cell survival in NSCLC. -- Abstract: Dickkopf1 (DKK1), a secreted protein involved in embryonic development, is a potent inhibitor of the Wnt signaling pathway and has been postulated to be a tumor suppressor or tumor promoter depending on the tumor type. In this study, we showed that DKK1 was expressed differently among non-small-cell lung cancer cell lines. The DKK1 expression level was much higher in A549 cells than in H460 cells. We revealed that blockage of DKK1 expression by silencing RNA in A549 cells caused up-regulation of intracellular reactive oxygen species (ROS) modulator (ROMO1) protein, followed by partial cell death, cell growth inhibition, and loss of epithelial–mesenchymal transition property caused by ROS, and it also increased ?-radiation sensitivity. DKK1 overexpression in H460 significantly inhibited cell survival with the decrease of ROMO1 level, which induced the decrease of cellular ROS. Thereafter, exogenous N-acetylcysteine, an antioxidant, or hydrogen peroxide, a pro-oxidant, partially rescued cells from death and growth inhibition. In each cell line, both overexpression and blockage of DKK1 not only elevated p-RB activation, which led to cell growth arrest, but also inactivated AKT/NF-kB, which increased radiation sensitivity and inhibited cell growth. This study is the first to demonstrate that strict modulation of DKK1 expression in different cell types partially maintains cell survival via tight regulation of the ROS-producing ROMO1 and radiation resistance.

Kim, In Gyu, E-mail: igkim@kaeri.re.kr [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Department of Radiation Biotechnology and Applied Radioisotope, University of Science and Technology (UST), 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Kim, Seo Yoen [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of) [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of); Kim, Hyun A; Kim, Jeong Yul [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of)] [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Lee, Jae Ha; Choi, Soo Im [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of) [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Department of Radiation Biotechnology and Applied Radioisotope, University of Science and Technology (UST), 989-111 Daedeok-daero, Yuseong-gu, Daejeon 305-353 (Korea, Republic of); Han, Jeong Ran; Kim, Kug Chan [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of)] [Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P.O. Box 105, Yuseong, Daejeon 305-600 (Korea, Republic of); Cho, Eun Wie [Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of)] [Biomedical Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806 (Korea, Republic of)

2014-01-03T23:59:59.000Z

173

Developmental time rather than local environment regulates the schedule of epithelial polarization in the zebrafish neural rod  

E-Print Network [OSTI]

within an initially solid neural rod primor- dium. Assembly of junctional complexes and polarity proteins at the midline marks the initiation of the colum- nar neuroepithelial architecture that is characteristic of vertebrate neural tubes. The emergence... information with the extrinsic envir- onment provides specificity of cell behavior in different tissues. Alternatively, an egg-timer-like mechanism may op- erate that consists of an intracellular factor that gradually increases or decreases in concentration...

Girdler, Gemma C; Araya, Claudio; Ren, Xiaoyun; Clarke, Jonathan DW

2013-03-24T23:59:59.000Z

174

Fuel cell arrangement  

DOE Patents [OSTI]

A fuel cell arrangement is provided wherein cylindrical cells of the solid oxide electrolyte type are arranged in planar arrays where the cells within a plane are parallel. Planes of cells are stacked with cells of adjacent planes perpendicular to one another. Air is provided to the interior of the cells through feed tubes which pass through a preheat chamber. Fuel is provided to the fuel cells through a channel in the center of the cell stack; the fuel then passes the exterior of the cells and combines with the oxygen-depleted air in the preheat chamber. 3 figs.

Isenberg, A.O.

1987-05-12T23:59:59.000Z

175

Fuel cell arrangement  

DOE Patents [OSTI]

A fuel cell arrangement is provided wherein cylindrical cells of the solid oxide electrolyte type are arranged in planar arrays where the cells within a plane are parallel. Planes of cells are stacked with cells of adjacent planes perpendicular to one another. Air is provided to the interior of the cells through feed tubes which pass through a preheat chamber. Fuel is provided to the fuel cells through a channel in the center of the cell stack; the fuel then passes the exterior of the cells and combines with the oxygen-depleted air in the preheat chamber.

Isenberg, Arnold O. (Forest Hills Boro, PA)

1987-05-12T23:59:59.000Z

176

DOE Fuel Cell Technologies Office: 2013 Fuel Cell Seminar and...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

DOE Fuel Cell Technologies Office: 2013 Fuel Cell Seminar and Energy Exposition DOE Fuel Cell Technologies Office: 2013 Fuel Cell Seminar and Energy Exposition Overview of DOE's...

177

DOE Fuel Cell Technologies Office Record 13012: Fuel Cell System...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cell Technologies Office Record 13012: Fuel Cell System Cost - 2013 DOE Fuel Cell Technologies Office Record 13012: Fuel Cell System Cost - 2013 This program record from the...

178

Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar...  

Broader source: Energy.gov (indexed) [DOE]

Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar and Exposition Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar and Exposition Presentation by...

179

Fuel cell-fuel cell hybrid system  

DOE Patents [OSTI]

A device for converting chemical energy to electricity is provided, the device comprising a high temperature fuel cell with the ability for partially oxidizing and completely reforming fuel, and a low temperature fuel cell juxtaposed to said high temperature fuel cell so as to utilize remaining reformed fuel from the high temperature fuel cell. Also provided is a method for producing electricity comprising directing fuel to a first fuel cell, completely oxidizing a first portion of the fuel and partially oxidizing a second portion of the fuel, directing the second fuel portion to a second fuel cell, allowing the first fuel cell to utilize the first portion of the fuel to produce electricity; and allowing the second fuel cell to utilize the second portion of the fuel to produce electricity.

Geisbrecht, Rodney A.; Williams, Mark C.

2003-09-23T23:59:59.000Z

180

Nanocrystal Solar Cells  

E-Print Network [OSTI]

Nov, 2005). Chapter 4 Hybrid solar cells with 3-dimensional5 All-inorganic nanocrystal solar cells 5.1 Introduction Inoperation of organic based solar cells and distinguish them

Gur, Ilan

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

the membrane for a PEM fuel cell would cost $5/ft (1990$) inmass-produced PEM fuel cell could cost $10/kW or less. Totalparameter for PEM fuel cells: thinner membranes cost less

Delucchi, Mark

1992-01-01T23:59:59.000Z

182

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

$ b materials cost, % a Fuel cell stack cost only. Includesof the cost of fuel-cell stacks, 1990$° Cost item GE Swan cAnnual maintenance cost of fuel cell stack and auxiliaries (

Delucchi, Mark

1992-01-01T23:59:59.000Z

183

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

Hydrogen Fuel Cell Vehicles UCD-ITS-RR-92-14 September bycost than both. Solar-hydrogen fuel- cell vehicles would becost than both. Solar-hydrogen fuel- cell vehicles would be

Delucchi, Mark

1992-01-01T23:59:59.000Z

184

Hydrogen Fuel Cell Vehicles  

E-Print Network [OSTI]

Hydrogen Fuel Cell Vehicles UCD-ITS-RR-92-14 September byet al. , 1988,1989 HYDROGEN FUEL-CELL VEHICLES: TECHNICALIn the FCEV, the hydrogen fuel cell could supply the "net"

Delucchi, Mark

1992-01-01T23:59:59.000Z

185

Thermal Management of Solar Cells  

E-Print Network [OSTI]

D. Mills, "Cooling of photovoltaic cells under concentratedelectric performance of a photovoltaic cells by cooling andSolar Cell A photovoltaic cell is a semiconductor that

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

186

SFTEL: Flow Cell | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Flow Cell EMSL's Subsurface Flow and Transport Experimental Laboratory offers several meter-scale flow cells and columns for research in saturated and unsaturated porous media....

187

Photovoltaic Cell Structure Basics  

Broader source: Energy.gov [DOE]

The actual structural design of a photovoltaic (PV), or solar cell, depends on the limitations of the material used in the PV cell.

188

Microfluidic fuel cells.  

E-Print Network [OSTI]

??Microfluidic fuel cell architectures are presented in this thesis. This work represents the mechanical and microfluidic portion of a microfluidic biofuel cell project. While the… (more)

Kjeang, Erik

2007-01-01T23:59:59.000Z

189

Snail/beta-catenin signaling protects breast cancer cells from hypoxia attack  

SciTech Connect (OSTI)

The tolerance of cancer cells to hypoxia depends on the combination of different factors – from increase of glycolysis (Warburg Effect) to activation of intracellular growth/apoptotic pathways. Less is known about the influence of epithelial–mesenchymal transition (EMT) and EMT-associated pathways on the cell sensitivity to hypoxia. The aim of this study was to explore the role of Snail signaling, one of the key EMT pathways, in the mediating of hypoxia response and regulation of cell sensitivity to hypoxia, using as a model in vitro cultured breast cancer cells. Earlier we have shown that estrogen-independent HBL-100 breast cancer cells differ from estrogen-dependent MCF-7 cells with increased expression of Snail1, and demonstrated Snail1 involvement into formation of hormone-resistant phenotype. Because Snail1 belongs to hypoxia-activated proteins, here we studied the influence of Snail1 signaling on the cell tolerance to hypoxia. We found that Snail1-enriched HBL-100 cells were less sensitive to hypoxia-induced growth suppression if compared with MCF-7 line (31% MCF-7 vs. 71% HBL-100 cell viability after 1% O{sub 2} atmosphere for 3 days). Snail1 knock-down enhanced the hypoxia-induced inhibition of cell proliferation giving the direct evidence of Snail1 involvement into cell protection from hypoxia attack. The protective effect of Snail1 was shown to be mediated, at least in a part, via beta-catenin which positively regulated expression of HIF-1-dependent genes. Finally, we found that cell tolerance to hypoxia was accompanied with the failure in the phosphorylation of AMPK – the key energy sensor, and demonstrated an inverse relationship between AMPK and Snail/beta-catenin signaling. Totally, our data show that Snail1 and beta-catenin, besides association with loss of hormone dependence, protect cancer cells from hypoxia and may serve as an important target in the treatment of breast cancer. Moreover, we suggest that the level of these proteins as well the level of AMPK phosphorylation may be considered as predictors of the tumor sensitivity to anti-angiogenic drugs. - Highlights: • Snail1 protects breast cancer cells from hypoxia. • Protective effect of Snail1 is mediated via ?-catenin/HIF-1 pathway. • Snail/?-catenin signaling is negatively controlled by the energy sensor – AMPK. • The failure in AMPK phosphorylation drives cells to the hypoxia-tolerant state.

Scherbakov, Alexander M., E-mail: alex.scherbakov@gmail.com [Laboratory of Clinical Biochemistry, Institute of Clinical Oncology, N.N. Blokhin Cancer Research Centre, Kashirskoye sh. 24, Moscow 115478 (Russian Federation); Stefanova, Lidia B.; Sorokin, Danila V.; Semina, Svetlana E. [Laboratory of Molecular Endocrinology, Institute of Carcinogenesis, N.N. Blokhin Cancer Research Centre, Kashirskoye sh. 24, Moscow 115478 (Russian Federation); Berstein, Lev M. [Laboratory of Oncoendocrinology, N.N. Petrov Research Institute of Oncology, St. Petersburg 197758 (Russian Federation); Krasil’nikov, Mikhail A. [Laboratory of Molecular Endocrinology, Institute of Carcinogenesis, N.N. Blokhin Cancer Research Centre, Kashirskoye sh. 24, Moscow 115478 (Russian Federation)

2013-12-10T23:59:59.000Z

190

POLYMER ELECTROLYTE FUEL CELLS  

E-Print Network [OSTI]

POLYMER ELECTROLYTE FUEL CELLS: The Gas Diffusion Layer Johannah Itescu Princeton University PRISM REU #12;PEM FUEL CELLS: A little background information I. What do fuel cells do? Generate electricity through chemical reaction #12;PEM FUEL CELLS: A little background information -+ + eHH 442 2 0244 22 He

Petta, Jason

191

Thermal Management of Solar Cells  

E-Print Network [OSTI]

cells by cooling and concentration techniques," inheat. Different techniques of cooling solar cells have been

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

192

Micro Fuel Cells Direct Methanol Fuel Cells  

E-Print Network [OSTI]

energy density of 1.5 Wh/cc; 1.5Wh/g = X5; x10 energy density of Li ion battery * Direct & complete Content (Wh) Volume(cm^3) Li-Ion Battery DMFC #12;Micro Fuel Cells TM State of MTI Micro Fuel Cells Energy Content (Wh) Volume(cm^3) Li-Ion Battery DMFC #12;Direct Methanol Fuel Cell Technology

193

Cell Stem Cell Highly Efficient Reprogramming  

E-Print Network [OSTI]

Alexander Meissner,4,5,14 George Q. Daley,2,3,4,5,8,15,16 Andrew S. Brack,5,6 James J. Collins,11,12,15 Chad Children's Hospital Boston, Boston, MA 02115, USA 4Department of Stem Cell and Regenerative Biology 5Harvard Stem Cell Institute Harvard University, Cambridge, MA 02138, USA 6Center of Regenerative Medicine

Collins, James J.

194

Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar...  

Energy Savers [EERE]

Update: 2010 Fuel Cell Seminar and Exposition Hydrogen and Fuel Cell Technologies Update: 2010 Fuel Cell Seminar and Exposition Presentation by Sunita Satyapal at the 2010 Fuel...

195

Cell-cell and cell-medium interactions in the growth of mouse embryonic stem cells  

E-Print Network [OSTI]

Embryonic stem cells serve as powerful models for the study of development and disease and hold enormous potential for future therapeutics. Due to the potential for embryonic stem cells (ESCs) to provide a variety of tissues ...

Mittal, Nikhil, 1979-

2010-01-01T23:59:59.000Z

196

Photovoltaic Cell Performance Basics  

Broader source: Energy.gov [DOE]

Photovoltaic (PV), or solar cells use the energy in sunlight to produce electricity. However, the amount of electricity produced depends on the quality of the light available and the performance of the PV cell.

197

fuel cells | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

fuel cells fuel cells Leads No leads are available at this time. The Molecular Bond: October 2014 The Molecular Bond newsletter banner October 2014 FROM THE DIRECTOR Read more...

198

NANOCOMPOSITE ENABLED SENSITIZED SOLAR CELL  

E-Print Network [OSTI]

by Dye-Sensitized Photovoltaic cells. Inorganic Chemistry,by Dye-Sensitized Photovoltaic Cells. Inorganic ChemistryThe characteristics of a photovoltaic cell. Generally,

Phuyal, Dibya

2012-01-01T23:59:59.000Z

199

Hybrid direct methanol fuel cells.  

E-Print Network [OSTI]

??A new type of fuel cell that combines the advantages of a proton exchange membrane fuel cells and anion exchange membrane fuel cells operated with… (more)

Joseph, Krishna Sathyamurthy

2012-01-01T23:59:59.000Z

200

NANOCOMPOSITE ENABLED SENSITIZED SOLAR CELL  

E-Print Network [OSTI]

efficiency in dye-sensitized solar cells based on Tio2Conversion by Dye-Sensitized Photovoltaic cells. InorganicConversion by Dye-Sensitized Photovoltaic Cells. Inorganic

Phuyal, Dibya

2012-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Electroluminescence in photovoltaic cell  

E-Print Network [OSTI]

Here we propose two methods to get electroluminescence images from photovoltaic cells in a school or home lab.

Petraglia, Antonio; 10.1088/0031-9120/46/5/F01

2011-01-01T23:59:59.000Z

202

Webinar: Fuel Cell Buses  

Broader source: Energy.gov [DOE]

Video recording and text version of the webinar titled, Fuel Cell Buses, originally presented on September 12, 2013.

203

Biomarkers of cell senescence  

DOE Patents [OSTI]

The present invention provides a biomarker system for the in vivo and in vitro assessment of cell senescence. In the method of the present invention, {beta}-galactosidase activity is utilized as a means by which cell senescence may be assessed either in in vitro cell cultures or in vivo. 1 fig.

Dirmi, G.P.; Campisi, J.; Peacocke, M.

1996-02-13T23:59:59.000Z

204

Biomarkers of cell senescence  

DOE Patents [OSTI]

The present invention provides a biomarker system for the in vivo and in vitro assessment of cell senescence. In the method of the present invention, {beta}-galactosidase activity is utilized as a means by which cell senescence may be assessed either in vitro cell cultures or in vivo. 1 fig.

Dimri, G.P.; Campisi, J.; Peacocke, M.

1998-08-18T23:59:59.000Z

205

FUEL CELLS FOR TRANSPORTATION  

E-Print Network [OSTI]

................................................................................................... 34 E. Cost Analyses of Fuel Cell Stacks/Systems ­ Arthur D. Little, Inc. ......................................... 40 F. DFMA Cost Estimates of Fuel-Cell/Reformer Systems at Low/Medium/High Production Rates&D of a Novel Breadboard Device Suitable for Carbon Monoxide Remediation in an Automotive PEM Fuel Cell Power

206

Rapidly refuelable fuel cell  

DOE Patents [OSTI]

This invention is directed to a metal-air fuel cell where the consumable metal anode is movably positioned in the cell and an expandable enclosure, or bladder, is used to press the anode into contact with separating spacers between the cell electrodes. The bladder may be depressurized to allow replacement of the anode when consumed.

Joy, Richard W. (Santa Clara, CA)

1983-01-01T23:59:59.000Z

207

Matrigel Basement Membrane Matrix influences expression of microRNAs in cancer cell lines  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Matrigel alters cancer cell line miRNA expression relative to culture on plastic. Black-Right-Pointing-Pointer Many identified Matrigel-regulated miRNAs are implicated in cancer. Black-Right-Pointing-Pointer miR-1290, -210, -32 and -29b represent a Matrigel-induced miRNA signature. Black-Right-Pointing-Pointer miR-32 down-regulates Integrin alpha 5 (ITGA5) mRNA. -- Abstract: Matrigel is a medium rich in extracellular matrix (ECM) components used for three-dimensional cell culture and is known to alter cellular phenotypes and gene expression. microRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have roles in cancer. While miRNA profiles of numerous cell lines cultured on plastic have been reported, the influence of Matrigel-based culture on cancer cell miRNA expression is largely unknown. This study investigated the influence of Matrigel on the expression of miRNAs that might facilitate ECM-associated cancer cell growth. We performed miRNA profiling by microarray using two colon cancer cell lines (SW480 and SW620), identifying significant differential expression of miRNAs between cells cultured in Matrigel and on plastic. Many of these miRNAs have previously been implicated in cancer-related processes. A common Matrigel-induced miRNA signature comprised of up-regulated miR-1290 and miR-210 and down-regulated miR-29b and miR-32 was identified using RT-qPCR across five epithelial cancer cell lines (SW480, SW620, HT-29, A549 and MDA-MB-231). Experimental modulation of these miRNAs altered expression of their known target mRNAs involved in cell adhesion, proliferation and invasion, in colon cancer cell lines. Furthermore, ITGA5 was identified as a novel putative target of miR-32 that may facilitate cancer cell interactions with the ECM. We propose that culture of cancer cell lines in Matrigel more accurately recapitulates miRNA expression and function in cancer than culture on plastic and thus is a valuable approach to the in vitro study of miRNAs.

Price, Karina J. [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia) [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); School of Medicine and Pharmacology, University of Western Australia, Nedlands, WA 6008 (Australia); Tsykin, Anna [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia) [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia); School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005 (Australia); Giles, Keith M. [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia)] [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); Sladic, Rosemary T. [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia)] [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia); Epis, Michael R. [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia)] [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); Ganss, Ruth [Laboratory for Cancer Medicine Angiogenesis Unit, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia)] [Laboratory for Cancer Medicine Angiogenesis Unit, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); Goodall, Gregory J. [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia) [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia); School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005 (Australia); Department of Medicine, University of Adelaide, Adelaide, SA 5005 (Australia); Leedman, Peter J., E-mail: peter.leedman@waimr.uwa.edu.au [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); School of Medicine and Pharmacology, University of Western Australia, Nedlands, WA 6008 (Australia)

2012-10-19T23:59:59.000Z

208

California: TetraCell Silicon Solar Cell Improves Efficiency...  

Energy Savers [EERE]

California: TetraCell Silicon Solar Cell Improves Efficiency, Wins R&D 100 Award California: TetraCell Silicon Solar Cell Improves Efficiency, Wins R&D 100 Award August 16, 2013 -...

209

DOE Fuel Cell Technologies Office Record 14012: Fuel Cell System...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

2: Fuel Cell System Cost - 2013 DOE Fuel Cell Technologies Office Record 14012: Fuel Cell System Cost - 2013 This program record from the U.S. Department of Energy's Fuel Cell...

210

Overexpression of Robo2 causes defects in the recruitment of metanephric mesenchymal cells and ureteric bud branching morphogenesis  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Overexpression of Robo2 caused reduced UB branching and glomerular number. Black-Right-Pointing-Pointer Fewer MM cells surrounding the UB after overexpression of Robo2 in vitro. Black-Right-Pointing-Pointer No abnormal Epithelial Morphology of UB or apoptosis of mm cells in the kidney. Black-Right-Pointing-Pointer Overexpression of Robo2 affected MM cells migration and caused UB deficit. Black-Right-Pointing-Pointer The reduced glomerular number can also be caused by fewer MM cells. -- Abstract: Roundabout 2 (Robo2) is a member of the membrane protein receptor family. The chemorepulsive effect of Slit2-Robo2 signaling plays vital roles in nervous system development and neuron migration. Slit2-Robo2 signaling is also important for maintaining the normal morphogenesis of the kidney and urinary collecting system, especially for the branching of the ureteric bud (UB) at the proper site. Slit2 or Robo2 mouse mutants exhibit multilobular kidneys, multiple ureters, and dilatation of the ureter, renal pelvis, and collecting duct system, which lead to vesicoureteral reflux. To understand the effect of Robo2 on kidney development, we used microinjection and electroporation to overexpress GFP-Robo2 in an in vitro embryonic kidney model. Our results show reduced UB branching and decreased glomerular number after in vitro Robo2 overexpression in the embryonic kidneys. We found fewer metanephric mesenchymal (MM) cells surrounding the UB but no abnormal morphology in the branching epithelial UB. Meanwhile, no significant change in MM proliferation or apoptosis was observed. These findings indicate that Robo2 is involved in the development of embryonic kidneys and that the normal expression of Robo2 can help maintain proper UB branching and glomerular morphogenesis. Overexpression of Robo2 leads to reduced UB branching caused by fewer surrounding MM cells, but MM cell apoptosis is not involved in this effect. Our study demonstrates that overexpression of Robo2 by microinjection in embryonic kidneys is an effective approach to study the function of Robo2.

Ji, Jiayao [Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV00088), The Chinese PLA General Hospital, Beijing 100853 (China) [Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV00088), The Chinese PLA General Hospital, Beijing 100853 (China); Medical College of NanKai University, Tianjin (China); Li, Qinggang; Xie, Yuansheng; Zhang, Xueguang; Cui, Shaoyuan; Shi, Suozhu [Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV00088), The Chinese PLA General Hospital, Beijing 100853 (China)] [Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV00088), The Chinese PLA General Hospital, Beijing 100853 (China); Chen, Xiangmei, E-mail: xmchen301@126.com [Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV00088), The Chinese PLA General Hospital, Beijing 100853 (China) [Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV00088), The Chinese PLA General Hospital, Beijing 100853 (China); Medical College of NanKai University, Tianjin (China)

2012-05-11T23:59:59.000Z

211

Webinar: Fuel Cell Mobile Lighting  

Broader source: Energy.gov [DOE]

Video recording of the Fuel Cell Technologies Office webinar, Fuel Cell Mobile Lighting, originally presented on November 13, 2012.

212

Effets de la prolactine sur la scrtion des lipides du lait dans les cellules pithliales mammaires  

E-Print Network [OSTI]

-en-Josas, France. Summary. Effect of prolactin on milk lipid secretion in lactating rabbit mammary gland epithelial secretion. These results show that the radioactivity of neosynthesized lipid constituents was modified during the secretion of milk lipids. Prolactin, which increased the total labelled lipids secreted, had

Boyer, Edmond

213

Concentrator silicon cell research  

SciTech Connect (OSTI)

This project continued the developments of high-efficiency silicon concentrator solar cells with the goal of achieving a cell efficiency in the 26 to 27 percent range at a concentration level of 150 suns of greater. The target efficiency was achieved with the new PERL (passivated emitter, rear locally diffused) cell structure, but only at low concentration levels around 20 suns. The PERL structure combines oxide passivation of both top and rear surfaces of the cells with small area contact to heavily doped regions on the top and rear surfaces. Efficiency in the 22 to 23 percent range was also demonstrated for large-area concentrator cells fabricated with the buried contact solar cell processing sequence, either when combined with prismatic covers or with other innovative approaches to reduce top contact shadowing. 19 refs.

Green, M.A.; Wenham, S.R.; Zhang, F.; Zhao, J.; Wang, A. [New South Wales Univ., Kensington (Australia). Solar Photovoltaic Lab.

1992-04-01T23:59:59.000Z

214

Direct hydrocarbon fuel cells  

DOE Patents [OSTI]

The direct electrochemical oxidation of hydrocarbons in solid oxide fuel cells, to generate greater power densities at lower temperatures without carbon deposition. The performance obtained is comparable to that of fuel cells used for hydrogen, and is achieved by using novel anode composites at low operating temperatures. Such solid oxide fuel cells, regardless of fuel source or operation, can be configured advantageously using the structural geometries of this invention.

Barnett, Scott A.; Lai, Tammy; Liu, Jiang

2010-05-04T23:59:59.000Z

215

Heterojunction solar cell  

DOE Patents [OSTI]

A high-efficiency single heterojunction solar cell is described wherein a thin emitter layer (preferably Ga[sub 0.52]In[sub 0.48]P) forms a heterojunction with a GaAs absorber layer. The conversion efficiency of the solar cell is at least 25.7%. The solar cell preferably includes a passivating layer between the substrate and the absorber layer. An anti-reflection coating is preferably disposed over the emitter layer. 1 fig.

Olson, J.M.

1994-08-30T23:59:59.000Z

216

Microcomposite Fuel Cell Membranes  

Broader source: Energy.gov [DOE]

Summary of microcomposite fuel cell membrane work presented to the High Temperature Membrane Working Group Meeting, Orlando FL, October 17, 2003

217

Molten salt lithium cells  

DOE Patents [OSTI]

Lithium-based cells are promising for applications such as electric vehicles and load-leveling for power plants since lithium is very electropositive and light weight. One type of lithium-based cell utilizes a molten salt electrolyte and is operated in the temperature range of about 400.degree.-500.degree. C. Such high temperature operation accelerates corrosion problems and a substantial amount of energy is lost through heat transfer. The present invention provides an electrochemical cell (10) which may be operated at temperatures between about 100.degree.-170.degree. C. Cell (10) comprises an electrolyte (16), which preferably includes lithium nitrate, and a lithium or lithium alloy electrode (12).

Raistrick, Ian D. (Menlo Park, CA); Poris, Jaime (Portola Valley, CA); Huggins, Robert A. (Stanford, CA)

1982-02-09T23:59:59.000Z

218

Molten salt lithium cells  

DOE Patents [OSTI]

Lithium-based cells are promising for applications such as electric vehicles and load-leveling for power plants since lithium is very electropositive and light weight. One type of lithium-based cell utilizes a molten salt electrolyte and is operated in the temperature range of about 400.degree.-500.degree. C. Such high temperature operation accelerates corrosion problems and a substantial amount of energy is lost through heat transfer. The present invention provides an electrochemical cell (10) which may be operated at temperatures between about 100.degree.-170.degree. C. Cell (10) comprises an electrolyte (16), which preferably includes lithium nitrate, and a lithium or lithium alloy electrode (12).

Raistrick, Ian D. (Menlo Park, CA); Poris, Jaime (Portola Valley, CA); Huggins, Robert A. (Stanford, CA)

1983-01-01T23:59:59.000Z

219

Molten salt lithium cells  

DOE Patents [OSTI]

Lithium-based cells are promising for applications such as electric vehicles and load-leveling for power plants since lithium is very electropositive and light weight. One type of lithium-based cell utilizes a molten salt electrolyte and is operated in the temperature range of about 400 to 500/sup 0/C. Such high temperature operation accelerates corrosion problems and a substantial amount of energy is lost through heat transfer. The present invention provides an electrochemical cell which may be operated at temperatures between about 100 to 170/sup 0/C. The cell is comprised of an electrolyte, which preferably includes lithium nitrate, and a lithium or lithium alloy electrode.

Raistrick, I.D.; Poris, J.; Huggins, R.A.

1980-07-18T23:59:59.000Z

220

Modelling microscale fuel cells.  

E-Print Network [OSTI]

??The focus of this work is to investigate transport phenomena in recently developed microscale fuel cell designs using computational fluid dynamics (CFD). Two microscale fuel… (more)

Bazylak, Aimy Ming Jii

2009-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Fuel Cell Technologies Overview  

Broader source: Energy.gov (indexed) [DOE]

Cells Key Benefits Very High Efficiency Reduced CO 2 Emissions Reduced Oil Use Reduced Air Pollution Fuel Flexibility * 40 - 60% (electrical) * > 70% (electrical, hybrid fuel...

222

Hydrogen Fuel Cells  

Fuel Cell Technologies Publication and Product Library (EERE)

The fuel cell — an energy conversion device that can efficiently capture and use the power of hydrogen — is the key to making it happen.

223

Electrochemical cell method  

DOE Patents [OSTI]

A secondary electrochemical cell is prepared by providing positive and negative electrodes having outer enclosures of rigid perforated electrically conductive material defining an internal compartment containing the electrode material in porous solid form. The electrodes are each immersed in molten electrolyte salt prior to cell assembly to incorporate the cell electrolyte. Following solidification of the electrolyte substantially throughout the porous volume of the electrode material, the electrodes are arranged in an alternating positive-negative array with interelectrode separators of porous frangible electrically insulative material. The completed array is assembled into the cell housing and sealed such that on heating the solidified electrolyte flows into the interelectrode separator.

Kaun, T.D.; Eshman, P.F.

1980-05-09T23:59:59.000Z

224

cell trait? Know your sickle cell trait status.  

E-Print Network [OSTI]

What is sickle cell trait? Know your sickle cell trait status. Engage in a slow and gradual experiencing unusual physical distress. People at high risk for having sickle cell trait are those whose countries. sickle cell trait is not a disease. Sickle cell trait is the inheritance of one gene for sickle

Devoto, Stephen H.

225

Hexavalent chromium at low concentration alters Sertoli cell barrier and connexin 43 gap junction but not claudin-11 and N-cadherin in the rat seminiferous tubule culture model  

SciTech Connect (OSTI)

Exposure to toxic metals, specifically those belonging to the nonessential group leads to human health defects and among them reprotoxic effects. The mechanisms by which these metals produce their negative effects on spermatogenesis have not been fully elucidated. By using the Durand's validated seminiferous tubule culture model, which mimics the in vivo situation, we recently reported that concentrations of hexavalent chromium, reported in the literature to be closed to that found in the blood circulation of men, increase the number of germ cell cytogenetic abnormalities. Since this metal is also known to affect cellular junctions, we investigated, in the present study, its potential influence on the Sertoli cell barrier and on junctional proteins present at this level such as connexin 43, claudin-11 and N-cadherin. Cultured seminiferous tubules in bicameral chambers expressed the three junctional proteins and ZO-1 for at least 12 days. Exposure to low concentrations of chromium (10 ?g/l) increased the trans-epithelial resistance without major changes of claudin-11 and N-cadherin expressions but strongly delocalized the gap junction protein connexin 43 from the membrane to the cytoplasm of Sertoli cells. The possibility that the hexavalent chromium-induced alteration of connexin 43 indirectly mediates the effect of the toxic metal on the blood–testis barrier dynamic is postulated. - Highlights: ? Influence of Cr(VI) on the Sertoli cell barrier and on junctional proteins ? Use of cultured seminiferous tubules in bicameral chambers ? Low concentrations of Cr(VI) (10 ?g/l) altered the trans-epithelial resistance. ? Cr(VI) did not alter claudin-11 and N-cadherin. ? Cr(VI) delocalized connexin 43 from the membrane to the cytoplasm of Sertoli cells.

Carette, Diane [INSERM U 1065, Team 5 “Physiopathology of Germ Cell Control: Genomic and Non Genomic Mechanisms” C3M, University of Nice Sophia Antipolis, Nice (France); UMR S775, University Paris Descartes, 45 rue des Saints Pères, 75006, Paris (France); Perrard, Marie-Hélène, E-mail: marie-helene.durand@ens-lyon.fr [Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Université Lyon I, CNRS, INRA, Ecole Normale Supérieure de Lyon, Lyon (France); Prisant, Nadia [University of Versailles/St Quentin-en-Yvelines (France); UMR S775, University Paris Descartes, 45 rue des Saints Pères, 75006, Paris (France); Gilleron, Jérome; Pointis, Georges [INSERM U 1065, Team 5 “Physiopathology of Germ Cell Control: Genomic and Non Genomic Mechanisms” C3M, University of Nice Sophia Antipolis, Nice (France); Segretain, Dominique [University of Versailles/St Quentin-en-Yvelines (France); UMR S775, University Paris Descartes, 45 rue des Saints Pères, 75006, Paris (France); Durand, Philippe [Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Université Lyon I, CNRS, INRA, Ecole Normale Supérieure de Lyon, Lyon (France); Kallistem SAS Ecole Normale Supérieure de Lyon, Lyon (France)

2013-04-01T23:59:59.000Z

226

Programmed cell death  

SciTech Connect (OSTI)

The purpose of this conference to provide a multidisciplinary forum for exchange of state-of-the-art information on the role programmed cell death plays in normal development and homeostasis of many organisms. This volume contains abstracts of papers in the following areas: invertebrate development; immunology/neurology; bcl-2 family; biochemistry; programmed cell death in viruses; oncogenesis; vertebrate development; and diseases.

NONE

1995-12-31T23:59:59.000Z

227

Fuel cell generator  

DOE Patents [OSTI]

High temperature solid oxide electrolyte fuel cell generators which allow controlled leakage among plural chambers in a sealed housing. Depleted oxidant and fuel are directly reacted in one chamber to combust remaining fuel and preheat incoming reactants. The cells are preferably electrically arranged in a series-parallel configuration.

Isenberg, Arnold O. (Forest Hills, PA)

1983-01-01T23:59:59.000Z

228

Mesangial cell biology  

SciTech Connect (OSTI)

Mesangial cells originate from the metanephric mesenchyme and maintain structural integrity of the glomerular microvascular bed and mesangial matrix homeostasis. In response to metabolic, immunologic or hemodynamic injury, these cells undergo apoptosis or acquire an activated phenotype and undergo hypertrophy, proliferation with excessive production of matrix proteins, growth factors, chemokines and cytokines. These soluble factors exert autocrine and paracrine effects on the cells or on other glomerular cells, respectively. MCs are primary targets of immune-mediated glomerular diseases such as IGA nephropathy or metabolic diseases such as diabetes. MCs may also respond to injury that primarily involves podocytes and endothelial cells or to structural and genetic abnormalities of the glomerular basement membrane. Signal transduction and oxidant stress pathways are activated in MCs and likely represent integrated input from multiple mediators. Such responses are convenient targets for therapeutic intervention. Studies in cultured MCs should be supplemented with in vivo studies as well as examination of freshly isolated cells from normal and diseases glomeruli. In addition to ex vivo morphologic studies in kidney cortex, cells should be studied in their natural environment, isolated glomeruli or even tissue slices. Identification of a specific marker of MCs should help genetic manipulation as well as selective therapeutic targeting of these cells. Identification of biological responses of MCs that are not mediated by the renin–angiotensin system should help development of novel and effective therapeutic strategies to treat diseases characterized by MC pathology.

Abboud, Hanna E., E-mail: Abboud@uthscsa.edu

2012-05-15T23:59:59.000Z

229

Collective migration of epithelial sheets  

E-Print Network [OSTI]

The varied movements of the epithelium play vital roles in the development and renewal of complex tissues, from the separation of tissues in the early embryo, to homeostasis in the adult. Their movement is intricately ...

Murrell, Michael Peter

2009-01-01T23:59:59.000Z

230

FORENSIC TECHNIQUES FOR CELL PHONES  

E-Print Network [OSTI]

June 2007 FORENSIC TECHNIQUES FOR CELL PHONES FORENSIC TECHNIQUES FOR CELL PHONES Shirley Radack cell phones are widely used for both personal and professional applications, the technology of cell forensics usually do not cover cell phones, especially those with advanced capabilities. The digital

231

Solid oxide fuel cell generator  

DOE Patents [OSTI]

A solid oxide fuel cell generator has a plenum containing at least two rows of spaced apart, annular, axially elongated fuel cells. An electrical conductor extending between adjacent rows of fuel cells connects the fuel cells of one row in parallel with each other and in series with the fuel cells of the adjacent row. 5 figures.

Di Croce, A.M.; Draper, R.

1993-11-02T23:59:59.000Z

232

Single-cell technologies for monitoring interactions between immune cells  

E-Print Network [OSTI]

Immune cells participate in dynamic cellular interactions that play a critical role in the defense against pathogens and the destruction of malignant cells. The vast heterogeneity of immune cells motivates the study of ...

Yamanaka, Yvonne J. (Yvonne Joy)

2014-01-01T23:59:59.000Z

233

Fuel Cells Vehicle Systems Analysis (Fuel Cell Freeze Investigation)  

SciTech Connect (OSTI)

Presentation on Fuel Cells Vehicle Systems Analysis (Fuel Cell Freeze Investigation) for the 2005 Hydrogen, Fuel Cells & Infrastructure Technologies Program Annual Review held in Arlington, Virginia on May 23-26, 2005.

Pesaran, A.; Kim, G.; Markel, T.; Wipke, K.

2005-05-01T23:59:59.000Z

234

Cochlear hair cell regeneration from neonatal mouse supporting cells  

E-Print Network [OSTI]

Unlike lower vertebrates, capable of spontaneous hair cell regeneration, mammals experience permanent sensorineural hearing loss following hair cell damage. Although low levels of hair cell regeneration have been demonstrated ...

Bramhall, Naomi F

2012-01-01T23:59:59.000Z

235

Nickel-induced down-regulation of {Delta}Np63 and its role in the proliferation of keratinocytes  

SciTech Connect (OSTI)

Epidemiological, animal, and cell studies have demonstrated that nickel compounds are human carcinogens. The mechanisms of their carcinogenic actions remain to be investigated. p63, a close homologue of the p53 tumor suppressor protein, has been linked to cell fate determination and/or maintenance of self-renewing populations in several epithelial tissues, including skin, mammary gland, and prostate. {Delta}Np63, a dominant negative isoform of p63, is amplified in a variety of epithelial tumors including squamous cell carcinomas and carcinomas of the prostate and mammary glands. The present study shows that nickel suppressed {Delta}Np63 expression in a short-time treatment (up to 48 h). Nickel treatment caused activation of NF-{kappa}B. Blockage of NF-{kappa}B partially reversed nickel-induced {Delta}Np63 suppression. Nickel decreased interferon regulatory factor (IRF) 3 and IRF7, IKK{epsilon}, and Sp100. Over-expression of IRF3 increased {Delta}Np63 expression suppressed by nickel. Nickel was able to activate p21, and its activation was offset by the over-expression of {Delta}Np63. In turn, elevated p63 expression counteracted the ability of nickel to restrict cell growth. The present study demonstrated that nickel decreased interferon regulatory proteins IRF3 and IRF7, and activated NF-{kappa}B, resulting in {Delta}Np63 suppression and then p21 up-regulation. {Delta}Np63 plays an important role in nickel-induced cell proliferation. - Highlights: > Ni suppressed {Delta}Np63 expression in HaCat cells. > Ni activated NF-{kappa}B, decreased expressions of IRF3 and IRF7, IKK{epsilon}, and Sp100. > Over-expression of IRF3 increased {Delta}Np63 expression suppressed by Ni. > Ni activated p21, and its activation was offset by over-expression of {Delta}Np63. > Elevated p63 expression counteracted the ability of nickel to restrict cell growth.

Zhang Zhuo, E-mail: zhuo.zhang@uky.edu [Department of Preventive Medicine and Environmental Health, University of Kentucky, 121 Washington Avenue, Lexington, KY 40536 (United States); Li Wenqi [Department of Preventive Medicine and Environmental Health, University of Kentucky, 121 Washington Avenue, Lexington, KY 40536 (United States); Cheng Senping [Graduate Center for Toxicology, University of Kentucky, 1095 VA Drive, Lexington, KY 40536 (United States); Yao Hua [Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003 (China); Zhang Fan; Chang Qingshan [Graduate Center for Toxicology, University of Kentucky, 1095 VA Drive, Lexington, KY 40536 (United States); Ke Zunji [Department of Internal Medicine, University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Wang Xin; Son, Young-Ok [Graduate Center for Toxicology, University of Kentucky, 1095 VA Drive, Lexington, KY 40536 (United States); Luo Jia [Department of Internal Medicine, University of Kentucky, 800 Rose Street, Lexington, KY 40536 (United States); Shi Xianglin [Graduate Center for Toxicology, University of Kentucky, 1095 VA Drive, Lexington, KY 40536 (United States)

2011-06-15T23:59:59.000Z

236

Diagnostic studies on Li-battery cells and cell components  

Broader source: Energy.gov (indexed) [DOE]

cells Disassembly of New and Aged Cells Electrode Surface & Bulk Analyses (ANL, BNL, LBNL) Electrolyte & Separator study (ANL, LBNL) Electrochemistry (ANL) Reference Electrode...

237

Red blood cell malformations Cell shapes Modeling and simulation of red blood cell light scattering  

E-Print Network [OSTI]

that have a "sickle" appearance (see figure to left). This malformed geometry prevents cells from traveling Red blood cell malformations Cell shapes Modeling and simulation of red blood cell light to various diseases and acute conditions, the shape and composition of erythrocytes (red blood cells

California at Berkeley, University of

238

Cell Patterning with Mucin Biopolymers  

E-Print Network [OSTI]

The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires ...

Crouzier, T.

239

Thermal Management of Solar Cells  

E-Print Network [OSTI]

Nanostructured Silicon- Based Solar Cells, 2013. X. C. Tong,heat exchangers, and solar cells," Sci-Tech News, vol. 65,in crystalline silicon solar cells," Renewable Energy, vol.

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

240

Imposed currents in galvanic cells  

E-Print Network [OSTI]

We analyze the steady-state behavior of a general mathematical model for reversible galvanic cells, such as redox flow cells, reversible solid oxide fuel cells, and rechargeable batteries. We consider not only operation ...

Biesheuvel, P. M.

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Power from the Fuel Cell  

E-Print Network [OSTI]

Power for Buildings Using Fuel-Cell Cars,” Proceedings ofwell as to drive down fuel-cell system costs through productthe potential advantages of fuel cells as clean and reliable

Lipman, Timothy E.

2000-01-01T23:59:59.000Z

242

Thermal Management of Solar Cells  

E-Print Network [OSTI]

is the ratio of the solar cell output power to the incidentmaximum power output at: The fill factor of a solar cell FFsolar cell temperature by about 15°C, which increases the output power

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

243

Energy 101: Fuel Cells | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Fuel Cells Energy 101: Fuel Cells Addthis Description Learn everything you need to know about fuel cells. Topic Hydrogen & Fuel Cells...

244

Fuel Cell Technologies Program Overview  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

CSD Workshop Washington, DC Fuel Cell Technologies Program Overview Dr. Sunita Satyapal Director, Fuel Cell Technologies Office Energy Efficiency and Renewable Energy U.S....

245

Slow elimination of phosphorylated histone {gamma}-H2AX from DNA of terminally differentiated mouse heart cells in situ  

SciTech Connect (OSTI)

Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around double-strand DNA breaks (DSBs) and this modification (called {gamma}-H2AX) may serve as a useful marker of genome damage and repair in terminally differentiated cells. Here using immunohistochemistry we studied kinetics of {gamma}-H2AX formation and elimination in the X-irradiated mouse heart and renal epithelial tissues in situ. Unirradiated tissues have 3-5% {gamma}-H2AX-positive cells and in tissues fixed 1 h after X-irradiation {gamma}-H2AX-positive nuclei are induced in a dose-dependent manner approaching 20-30% after 3 Gy of IR. Analysis of mouse tissues at different times after 3 Gy of IR showed that maximal induction of {gamma}-H2AX in heart is observed 20 min after IR and then is decreased slowly with about half remaining 23 h later. In renal epithelium maximum of the {gamma}-H2AX-positive cells is observed 40 min after IR and then decreases to control values in 23 h. This indicates that there are significant variations between non-proliferating mammalian tissues in the initial H2AX phosphorylation rate as well as in the rate of {gamma}-H2AX elimination after X-irradiation, which should be taken into account in the analysis of radiation responses.

Gavrilov, Boris [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Vezhenkova, Irina [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Firsanov, Denis [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Solovjeva, Liudmila [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Svetlova, Maria [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Mikhailov, Vyacheslav [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Tomilin, Nikolai [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation)]. E-mail: nvtom@hotmail.com

2006-09-08T23:59:59.000Z

246

Solar cell array interconnects  

DOE Patents [OSTI]

Electrical interconnects are disclosed for solar cells or other electronic components using a silver-silicone paste or a lead-tin (Pb-Sn) no-clean fluxless solder cream, whereby the high breakage of thin (<6 mil thick) solar cells using conventional solder interconnect is eliminated. The interconnects of this invention employs copper strips which are secured to the solar cells by a silver-silicone conductive paste which can be used at room temperature, or by a Pb-Sn solder cream which eliminates undesired residue on the active surfaces of the solar cells. Electrical testing using the interconnects of this invention has shown that no degradation of the interconnects developed under high current testing, while providing a very low contact resistance value. 4 figs.

Carey, P.G.; Thompson, J.B.; Colella, N.J.; Williams, K.A.

1995-11-14T23:59:59.000Z

247

Thin film photovoltaic cells  

DOE Patents [OSTI]

A solar cell has as its transparent electrical contact a grid made from a non-noble metal by providing a layer of copper oxide between the transparent electrical contact and the absorber-generator.

Rothwarf, Allen (Philadelphia, PA)

1981-01-01T23:59:59.000Z

248

Solar cell array interconnects  

DOE Patents [OSTI]

Electrical interconnects for solar cells or other electronic components using a silver-silicone paste or a lead-tin (Pb-Sn) no-clean fluxless solder cream, whereby the high breakage of thin (<6 mil thick) solar cells using conventional solder interconnect is eliminated. The interconnects of this invention employs copper strips which are secured to the solar cells by a silver-silicone conductive paste which can be used at room temperature, or by a Pb-Sn solder cream which eliminates undesired residue on the active surfaces of the solar cells. Electrical testing using the interconnects of this invention has shown that no degradation of the interconnects developed under high current testing, while providing a very low contact resistance value.

Carey, Paul G. (Mountain View, CA); Thompson, Jesse B. (Brentwood, CA); Colella, Nicolas J. (Livermore, CA); Williams, Kenneth A. (Livermore, CA)

1995-01-01T23:59:59.000Z

249

Photovoltaic solar cell  

DOE Patents [OSTI]

A photovoltaic solar cell for generating electricity from sunlight is disclosed. The photovoltaic solar cell comprises a plurality of spaced-apart point contact junctions formed in a semiconductor body to receive the sunlight and generate the electicity therefrom, the plurality of spaced-apart point contact junctions having a first plurality of regions having a first doping type and a second plurality of regions having a second doping type. In addition, the photovoltaic solar cell comprises a first electrical contact electrically connected to each of the first plurality of regions and a second electrical contact electrically connected to each of the second plurality of regions, as well as a passivation layer covering major surfaces and sidewalls of the photovoltaic solar cell.

Nielson, Gregory N; Okandan, Murat; Cruz-Campa, Jose Luis; Resnick, Paul J

2013-11-26T23:59:59.000Z

250

Photovoltaic solar cell  

DOE Patents [OSTI]

A photovoltaic solar cell for generating electricity from sunlight is disclosed. The photovoltaic solar cell comprises a plurality of spaced-apart point contact junctions formed in a semiconductor body to receive the sunlight and generate the electricity therefrom, the plurality of spaced-apart point contact junctions having a first plurality of regions having a first doping type and a second plurality of regions having a second doping type. In addition, the photovoltaic solar cell comprises a first electrical contact electrically connected to each of the first plurality of regions and a second electrical contact electrically connected to each of the second plurality of regions, as well as a passivation layer covering major surfaces and sidewalls of the photovoltaic solar cell.

Nielson, Gregory N; Cruz-Campa, Jose Luis; Okandan, Murat; Resnick, Paul J

2014-05-20T23:59:59.000Z

251

Fuel cell water transport  

DOE Patents [OSTI]

The moisture content and temperature of hydrogen and oxygen gases is regulated throughout traverse of the gases in a fuel cell incorporating a solid polymer membrane. At least one of the gases traverses a first flow field adjacent the solid polymer membrane, where chemical reactions occur to generate an electrical current. A second flow field is located sequential with the first flow field and incorporates a membrane for effective water transport. A control fluid is then circulated adjacent the second membrane on the face opposite the fuel cell gas wherein moisture is either transported from the control fluid to humidify a fuel gas, e.g., hydrogen, or to the control fluid to prevent excess water buildup in the oxidizer gas, e.g., oxygen. Evaporation of water into the control gas and the control gas temperature act to control the fuel cell gas temperatures throughout the traverse of the fuel cell by the gases.

Vanderborgh, Nicholas E. (Los Alamos, NM); Hedstrom, James C. (Los Alamos, NM)

1990-01-01T23:59:59.000Z

252

Microbubble cell actuator  

E-Print Network [OSTI]

The field of microsystems technology is rapidly growing, and expanding its horizons to applications in bioengineering. Currently, there are no cell analysis systems that facilitate the collection of dynamic responses for ...

Braff, Rebecca A. (Rebecca Alice)

1999-01-01T23:59:59.000Z

253

Compliant fuel cell system  

DOE Patents [OSTI]

A fuel cell assembly comprising at least one metallic component, at least one ceramic component and a structure disposed between the metallic component and the ceramic component. The structure is configured to have a lower stiffness compared to at least one of the metallic component and the ceramic component, to accommodate a difference in strain between the metallic component and the ceramic component of the fuel cell assembly.

Bourgeois, Richard Scott (Albany, NY); Gudlavalleti, Sauri (Albany, NY)

2009-12-15T23:59:59.000Z

254

Composite fuel cell membranes  

SciTech Connect (OSTI)

A bilayer or trilayer composite ion exchange membrane suitable for use in a fuel cell. The composite membrane has a high equivalent weight thick layer in order to provide sufficient strength and low equivalent weight surface layers for improved electrical performance in a fuel cell. In use, the composite membrane is provided with electrode surface layers. The composite membrane can be composed of a sulfonic fluoropolymer in both core and surface layers.

Plowman, Keith R. (Lake Jackson, TX); Rehg, Timothy J. (Lake Jackson, TX); Davis, Larry W. (West Columbia, TX); Carl, William P. (Marble Falls, TX); Cisar, Alan J. (Cypress, TX); Eastland, Charles S. (West Columbia, TX)

1997-01-01T23:59:59.000Z

255

Ice electrode electrolytic cell  

DOE Patents [OSTI]

This invention relates to a method and apparatus for removing heavy metals from waste water, soils, or process streams by electrolytic cell means. The method includes cooling a cell cathode to form an ice layer over the cathode and then applying an electric current to deposit a layer of the heavy metal over the ice. The metal is then easily removed after melting the ice. In a second embodiment, the same ice-covered electrode can be employed to form powdered metals.

Glenn, David F. (Idaho Falls, ID); Suciu, Dan F. (Idaho Falls, ID); Harris, Taryl L. (Idaho Falls, ID); Ingram, Jani C. (Idaho Falls, ID)

1993-01-01T23:59:59.000Z

256

Ice electrode electrolytic cell  

DOE Patents [OSTI]

This invention relates to a method and apparatus for removing heavy metals from waste water, soils, or process streams by electrolytic cell means. The method includes cooling a cell cathode to form an ice layer over the cathode and then applying an electric current to deposit a layer of the heavy metal over the ice. The metal is then easily removed after melting the ice. In a second embodiment, the same ice-covered electrode can be employed to form powdered metals.

Glenn, D.F.; Suciu, D.F.; Harris, T.L.; Ingram, J.C.

1993-04-06T23:59:59.000Z

257

Composite fuel cell membranes  

DOE Patents [OSTI]

A bilayer or trilayer composite ion exchange membrane is described suitable for use in a fuel cell. The composite membrane has a high equivalent weight thick layer in order to provide sufficient strength and low equivalent weight surface layers for improved electrical performance in a fuel cell. In use, the composite membrane is provided with electrode surface layers. The composite membrane can be composed of a sulfonic fluoropolymer in both core and surface layers.

Plowman, K.R.; Rehg, T.J.; Davis, L.W.; Carl, W.P.; Cisar, A.J.; Eastland, C.S.

1997-08-05T23:59:59.000Z

258

Aluminum reduction cell electrode  

DOE Patents [OSTI]

The invention is directed to cathode modules comprised of refractory hard metal materials, such as TiB[sub 2], for an electrolytic cell for the reduction of alumina wherein the modules may be installed and replaced during operation of the cell and wherein the structure of the cathode modules is such that the refractory hard metal materials are not subjected to externally applied forces or rigid constraints. 9 figs.

Goodnow, W.H.; Payne, J.R.

1982-09-14T23:59:59.000Z

259

Nighttime solar cell  

SciTech Connect (OSTI)

Currently photovoltaic (PV) cells convert solar energy into electrical energy at an efficiency of about 18%, with the maximum conversion rate taking place around noon on a cloudless day. In many applications, the PV cells are utilized to recharge a stand-by battery pack that provides electrical energy at night or on cloudy days. Increasing the utilization of the panel array area by producing electrical power at night will reduce the amount of required electrical energy storage for a given array size and increase system reliability. Thermoelectric generators (TEG) are solid state devices that convert thermal energy into electrical energy. Using the nighttime sky, or deep space, with an effective temperature of 3.5 K as a cold sink, the TEG presented here can produce electrical power at night. The hot junction is supplied energy by the ambient air temperature or some other warm temperature source. The cold junction of the TEG is insulated from the surroundings by a vacuum cell, improving its overall effectiveness. Combining the TEG with the PV cell, a unique solid state device is developed that converts electromagnetic radiant energy into usable electrical energy. The thermoelectric-photovoltaic (TEPV) cell, or the Nighttime Solar Cell, is a direct energy conversion device that produces electrical energy both at night and during the day.

Parise, R.J.

1998-07-01T23:59:59.000Z

260

Monolithic tandem solar cell  

SciTech Connect (OSTI)

It is an object of the invention to provide a monolithic tandem photovoltaic solar cell which is highly radiation resistant and efficient; in which the energy bandgap of the lower subcell can be tailored for specific applications; solar cell comprising layers of InP and GaInAsP (or GaInAs), where said photovoltaic cell is useful, for example, in space power applications; having an improved power-to-mass ratio; in which subcells are lattice-matches; and are both two terminal and three terminal monolithic tandem photovoltaic solar cells. To achieve the foregoing and other objects and in accordance with the purpose of the present invention, as embodied and broadly described herein, the monolithic tandem photovoltaic solar cell may comprise; (a) an InP substrate having an upper surface; (b) a first photoactive subcell on the upper surface of the InP substrate; wherein the first subcell comprises GaInAs (which could include GaInAsP) and includes a homojunction; and (c) a second photoactive subcell on the first subcell; wherein the second subcell comprises InP and includes a homojunction. The cell is described in detail. 5 figs., 2 tabs.

Wanlass, M.W.

1989-11-03T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

What do grid cells contribute to place cell firing?  

E-Print Network [OSTI]

What do grid cells contribute to place cell firing? Daniel Bush1,2 , Caswell Barry3 , and Neil to be generated by input from entorhinal grid cell modules with differing spatial scales. Here, we review recent and direction to environmental boundaries, while grid cells provide a complementary self-motion related input

Burgess, Neil

262

Cadmium induces carcinogenesis in BEAS-2B cells through ROS-dependent activation of PI3K/AKT/GSK-3?/?-catenin signaling  

SciTech Connect (OSTI)

Cadmium has been widely used in industry and is known to be carcinogenic to humans. Although it is widely accepted that chronic exposure to cadmium increases the incidence of cancer, the mechanisms underlying cadmium-induced carcinogenesis are unclear. The main aim of this study was to investigate the role of reactive oxygen species (ROS) in cadmium-induced carcinogenesis and the signal transduction pathways involved. Chronic exposure of human bronchial epithelial BEAS-2B cells to cadmium induced cell transformation, as evidenced by anchorage-independent growth in soft agar and clonogenic assays. Chronic cadmium treatment also increased the potential of these cells to invade and migrate. Injection of cadmium-stimulated cells into nude mice resulted in the formation of tumors. In contrast, the cadmium-mediated increases in colony formation, cell invasion and migration were prevented by transfection with catalase, superoxide dismutase-1 (SOD1), or SOD2. In particular, chronic cadmium exposure led to activation of signaling cascades involving PI3K, AKT, GSK-3?, and ?-catenin and transfection with each of the above antioxidant enzymes markedly inhibited cadmium-mediated activation of these signaling proteins. Inhibitors specific for AKT or ?-catenin almost completely suppressed the cadmium-mediated increase in total and active ?-catenin proteins and colony formation. Moreover, there was a marked induction of AKT, GSK-3?, ?-catenin, and carcinogenic markers in tumor tissues formed in mice after injection with cadmium-stimulated cells. Collectively, our findings suggest a direct involvement of ROS in cadmium-induced carcinogenesis and implicate a role of AKT/GSK-3?/?-catenin signaling in this process. -- Highlights: ? Chronic exposure to cadmium induces carcinogenic properties in BEAS-2B cells. ? ROS involved in cadmium-induced tumorigenicity of BEAS-2B cells. ? Cadmium activates ROS-dependent AKT/GSK-3?/?-catenin-mediated signaling. ? ROS-dependent signaling as potential therapeutic targets in cadmium carcinogenesis.

Son, Young-Ok; Wang, Lei; Poyil, Pratheeshkumar; Budhraja, Amit; Hitron, J. Andrew; Zhang, Zhuo [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY (United States)] [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY (United States); Lee, Jeong-Chae [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY (United States) [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY (United States); School of Dentistry and Institute of Oral Biosciences (BK21 program), Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Shi, Xianglin, E-mail: xshi5@email.uky.edu [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY (United States)] [Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY (United States)

2012-10-15T23:59:59.000Z

263

FUEL CELL TECHNOLOGIES PROGRAM Technologies  

E-Print Network [OSTI]

and fuel cells offer great promise for our energy future. Fuel cell vehicles are not yet commercially, such as a hydrogen fueling station or hydrogen fuel cell vehicle. Technology validation does not certify, and the Federal Government to evaluate hydrogen fuel cell vehicle and infrastructure technologies together in real

264

Seventh Edition Fuel Cell Handbook  

SciTech Connect (OSTI)

Provides an overview of fuel cell technology and research projects. Discusses the basic workings of fuel cells and their system components, main fuel cell types, their characteristics, and their development status, as well as a discussion of potential fuel cell applications.

NETL

2004-11-01T23:59:59.000Z

265

National Fuel Cell Research Center  

E-Print Network [OSTI]

National Fuel Cell Research Center www.nfcrc.uci.edu MOLTEN CARBONATE FUEL CELLS STEADY STATE MODELING OF MOLTEN CARBONATE FUEL CELLS FOR SYSTEM PERFORMANCE ANALYSES OVERVIEW Development of steady state and dynamic simulation capabilities for molten carbonate fuel cell (MCFC) technology is being

Mease, Kenneth D.

266

Shipboard Fuel Cell Biofuel Introduction  

E-Print Network [OSTI]

Update FuelCell Energy (Frank Wolak) 1230 PNNL SOFC Power Systems Update PNNL (Larry Chick) 1300 PEM Lessons Learned · System Generic Concepts (PEM, HT PEM, MCFC, SOFC) · Shipboard Fuel Cell CharacteristicsShipboard Fuel Cell ­ Biofuel Introduction: This program will demonstrate a shipboard fuel cell

267

Breakthrough Vehicle Development - Fuel Cells  

Fuel Cell Technologies Publication and Product Library (EERE)

Document describing research and development program for fuel cell power systems for transportation applications.

268

Sulphoraphane, a naturally occurring isothiocyanate induces apoptosis in breast cancer cells by targeting heat shock proteins  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer HSPs (27, 70 and 90) and HSF1 are overexpressed in MCF-7 and MDA-MB-231 cells. Black-Right-Pointing-Pointer Sulphoraphane, a natural isothiocyanate inhibited HSPs and HSF1 expressions. Black-Right-Pointing-Pointer Inhibition of HSPs and HSF1 lead to regulation of apoptotic proteins. Black-Right-Pointing-Pointer Alteration of apoptotic proteins activate of caspases particularly caspase 3 and 9 leading to induction of apoptosis. Black-Right-Pointing-Pointer Alteration of apoptotic proteins induce caspases leading to induction of apoptosis. -- Abstract: Heat shock proteins (HSPs) are involved in protein folding, aggregation, transport and/or stabilization by acting as a molecular chaperone, leading to inhibition of apoptosis by both caspase dependent and/or independent pathways. HSPs are overexpressed in a wide range of human cancers and are implicated in tumor cell proliferation, differentiation, invasion and metastasis. HSPs particularly 27, 70, 90 and the transcription factor heat shock factor1 (HSF1) play key roles in the etiology of breast cancer and can be considered as potential therapeutic target. The present study was designed to investigate the role of sulphoraphane, a natural isothiocyanate on HSPs (27, 70, 90) and HSF1 in two different breast cancer cell lines MCF-7 and MDA-MB-231 cells expressing wild type and mutated p53 respectively, vis-a-vis in normal breast epithelial cell line MCF-12F. It was furthermore investigated whether modulation of HSPs and HSF1 could induce apoptosis in these cells by altering the expressions of p53, p21 and some apoptotic proteins like Bcl-2, Bax, Bid, Bad, Apaf-1 and AIF. Sulphoraphane was found to down-regulate the expressions of HSP70, 90 and HSF1, though the effect on HSP27 was not pronounced. Consequences of HSP inhibition was upregulation of p21 irrespective of p53 status. Bax, Bad, Apaf-1, AIF were upregulated followed by down-regulation of Bcl-2 and this effect was prominent in MCF-7 than in MDA-MB-231. However, very little change in the expression of Bid was observed. Alteration in Bcl-2 Bax ratio resulted in the release of cytochrome c from mitochondria and activation of caspases 3 and 9 which are in agreement with apoptotic index values. Sulphoraphane therefore can be regarded as a potent inducer of apoptosis due to HSP modulation in breast cancer cells.

Sarkar, Ruma; Mukherjee, Sutapa [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India); Biswas, Jaydip [Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India); Roy, Madhumita, E-mail: mitacnci@yahoo.co.in [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)

2012-10-12T23:59:59.000Z

269

Cell Phone Detection Techniques  

SciTech Connect (OSTI)

A team composed of Rick Pratt, Dave Puczyki, Kyle Bunch, Ryan Slaugh, Morris Good, and Doug McMakin teamed together to attempt to exploit cellular telephone features and detect if a person was carrying a cellular telephone into a Limited Area. The cell phone’s electromagnetic properties were measured, analyzed, and tested in over 10 different ways to determine if an exploitable signature exists. The method that appears to have the most potential for success without adding an external tag is to measure the RF spectrum, not in the cell phone band, but between 240 and 400MHz. Figures 1- 7 show the detected signal levels from cell phones from three different manufacturers.

Pratt, Richard M.; Bunch, Kyle J.; Puzycki, David J.; Slaugh, Ryan W.; Good, Morris S.; McMakin, Douglas L.

2007-10-01T23:59:59.000Z

270

Monolithic tandem solar cell  

DOE Patents [OSTI]

A single-crystal, monolithic, tandem, photovoltaic solar cell is described which includes (a) an InP substrate having upper and lower surfaces, (b) a first photoactive subcell on the upper surface of the InP substrate, and (c) a second photoactive subcell on the first subcell. The first photoactive subcell is GaInAsP of defined composition. The second subcell is InP. The two subcells are lattice matched. The solar cell can be provided as a two-terminal device or a three-terminal device.

Wanlass, Mark W. (Golden, CO)

1991-01-01T23:59:59.000Z

271

Separators for electrochemical cells  

DOE Patents [OSTI]

Provided are separators for use in an electrochemical cell comprising (a) an inorganic oxide and (b) an organic polymer, wherein the inorganic oxide comprises organic substituents. Preferably, the inorganic oxide comprises an hydrated aluminum oxide of the formula Al.sub.2O.sub.3.xH.sub.2O, wherein x is less than 1.0, and wherein the hydrated aluminum oxide comprises organic substituents, preferably comprising a reaction product of a multifunctional monomer and/or organic carbonate with an aluminum oxide, such as pseudo-boehmite and an aluminum oxide. Also provided are electrochemical cells comprising such separators.

Carlson, Steven Allen; Anakor, Ifenna Kingsley

2014-11-11T23:59:59.000Z

272

Superlattice cascade solar cell  

SciTech Connect (OSTI)

This paper reports progress toward realization of a new cascade solar cell structure whose chief advantages over other present concepts are: use of silicon for the substrate and low bandgap cell; avoidance of the necessity of lattice matching; and incorporation of a GaAs/GaP superlattice to enhance efficiency and provide a low-resistance connecting junction. Details of the design and operation of an OMCVD system for growing this structure are presented. Results of experiments to optimize layer thickness, compositional uniformity, and surface morphology are described.

Wanlass, M.W.; Blakeslee, A.E.

1982-09-01T23:59:59.000Z

273

Monolithic tandem solar cell  

SciTech Connect (OSTI)

This patent describes a single-crystal, monolithic, tandem, photovoltaic solar cell which includes an InP substrate having an upper and lower surfaces, a first photoactive subcell on the upper surface of the InP substrate, and a second photoactive subcell on the first subcell. The first photovoltaic subcell is GaInAsP of defined composition. The second subcell is InP. The two subcells are lattice matched. The solar cell can be provided as a two- terminal device or a three-terminal device.

Wanlass, M.W.

1991-05-28T23:59:59.000Z

274

Solar Cells: Spin-Cast Bulk Heterojunction Solar Cells: A Dynamical...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Solar Cells: Spin-Cast Bulk Heterojunction Solar Cells: A Dynamical Investigation Solar Cells: Spin-Cast Bulk Heterojunction Solar Cells: A Dynamical Investigation Print Wednesday,...

275

Pancreatic stellate cells enhance stem cell-like phenotypes in pancreatic cancer cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Pancreatic stellate cells (PSCs) promote the progression of pancreatic cancer. Black-Right-Pointing-Pointer Pancreatic cancer cells co-cultured with PSCs showed enhanced spheroid formation. Black-Right-Pointing-Pointer Expression of stem cell-related genes ABCG2, Nestin and LIN28 was increased. Black-Right-Pointing-Pointer Co-injection of PSCs enhanced tumorigenicity of pancreatic cancer cells in vivo. Black-Right-Pointing-Pointer This study suggested a novel role of PSCs as a part of the cancer stem cell niche. -- Abstract: The interaction between pancreatic cancer cells and pancreatic stellate cells (PSCs), a major profibrogenic cell type in the pancreas, is receiving increasing attention. There is accumulating evidence that PSCs promote the progression of pancreatic cancer by increasing cancer cell proliferation and invasion as well as by protecting them from radiation- and gemcitabine-induced apoptosis. Recent studies have identified that a portion of cancer cells, called 'cancer stem cells', within the entire cancer tissue harbor highly tumorigenic and chemo-resistant phenotypes, which lead to the recurrence after surgery or re-growth of the tumor. The mechanisms that maintain the 'stemness' of these cells remain largely unknown. We hypothesized that PSCs might enhance the cancer stem cell-like phenotypes in pancreatic cancer cells. Indirect co-culture of pancreatic cancer cells with PSCs enhanced the spheroid-forming ability of cancer cells and induced the expression of cancer stem cell-related genes ABCG2, Nestin and LIN28. In addition, co-injection of PSCs enhanced tumorigenicity of pancreatic cancer cells in vivo. These results suggested a novel role of PSCs as a part of the cancer stem cell niche.

Hamada, Shin [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan)] [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan); Masamune, Atsushi, E-mail: amasamune@med.tohoku.ac.jp [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan)] [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan); Takikawa, Tetsuya; Suzuki, Noriaki; Kikuta, Kazuhiro; Hirota, Morihisa [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan)] [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan); Hamada, Hirofumi [Laboratory of Oncology, Department of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji (Japan)] [Laboratory of Oncology, Department of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji (Japan); Kobune, Masayoshi [Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo (Japan)] [Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo (Japan); Satoh, Kennichi [Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori (Japan)] [Division of Cancer Stem Cell, Miyagi Cancer Center Research Institute, Natori (Japan); Shimosegawa, Tooru [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan)] [Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai (Japan)

2012-05-04T23:59:59.000Z

276

Thin film photovoltaic cell  

DOE Patents [OSTI]

A thin film photovoltaic cell having a transparent electrical contact and an opaque electrical contact with a pair of semiconductors therebetween includes utilizing one of the electrical contacts as a substrate and wherein the inner surface thereof is modified by microroughening while being macro-planar.

Meakin, John D. (Newark, DE); Bragagnolo, Julio (Newark, DE)

1982-01-01T23:59:59.000Z

277

Introgression & mapping Fiber cell  

E-Print Network [OSTI]

Germplasm Introgression Genomics & mapping Fiber cell initiation Radiation hybrid (RH) mapping and breeding. Research activities commonly include plant breeding, genetics, genomics, cytogenetics, molecular methods. (C, S) · Contribute uniquely to genomics and its relevance to genetic improvement (C,S) · Harness

278

Cell patterning technology for controlling the stem cell microenvironment  

E-Print Network [OSTI]

Embryonic stem cells serve as powerful models for the study of development and disease and hold enormous potential for future therapeutics. Yet, over two decades after mouse embryonic stem cells (mESCs) were first isolated, ...

Rosenthal, Adam D. (Adam David), 1978-

2007-01-01T23:59:59.000Z

279

EE580 Solar Cells Todd J. Kaiser  

E-Print Network [OSTI]

7/21/2010 1 EE580 ­ Solar Cells Todd J. Kaiser · Lecture 06 · Solar Cell Materials & Structures 1Montana State University: Solar Cells Lecture 6: Solar Cells Solar Cell Technologies · A) Crystalline Silicon · B) Thin Film · C) Group III-IV Cells 2Montana State University: Solar Cells Lecture 6: Solar

Kaiser, Todd J.

280

Fuel Cell Handbook, Fourth Edition  

SciTech Connect (OSTI)

Robust progress has been made in fuel cell technology since the previous edition of the Fuel Cell Handbook was published in January 1994. This Handbook provides a foundation in fuel cells for persons wanting a better understanding of the technology, its benefits, and the systems issues that influence its application. Trends in technology are discussed, including next-generation concepts that promise ultra high efficiency and low cost, while providing exceptionally clean power plant systems. Section 1 summarizes fuel cell progress since the last edition and includes existing power plant nameplate data. Section 2 addresses the thermodynamics of fuel cells to provide an understanding of fuel cell operation at two levels (basic and advanced). Sections 3 through 6 describe the four major fuel cell types and their performance based on cell operating conditions. The section on polymer electrolyte membrane fuel cells has been added to reflect their emergence as a significant fuel cell technology. Phosphoric acid, molten carbonate, and solid oxide fuel cell technology description sections have been updated from the previous edition. New information indicates that manufacturers have stayed with proven cell designs, focusing instead on advancing the system surrounding the fuel cell to lower life cycle costs. Section 7, Fuel Cell Systems, has been significantly revised to characterize near-term and next-generation fuel cell power plant systems at a conceptual level of detail. Section 8 provides examples of practical fuel cell system calculations. A list of fuel cell URLs is included in the Appendix. A new index assists the reader in locating specific information quickly.

Stauffer, D.B; Hirschenhofer, J.H.; Klett, M.G.; Engleman, R.R.

1998-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Sequencing the transcriptome of milk production: milk trumps mammary tissue  

E-Print Network [OSTI]

important for milk lipid secretion. J Biol Chem 53. Russellin milk lipid formation and secretion. Trends Endocrinol

2013-01-01T23:59:59.000Z

282

Laminin and biomimetic extracellular elasticity enhance functional differentiation in mammary  

E-Print Network [OSTI]

Brownfield1 , Derek C Radisky1,6 , Carlos Bustamante2,3,4 and Mina J Bissell1, * 1 Life Sciences Division

Nelson, Celeste M.

283

MicroRNA expression in canine mammary cancer  

E-Print Network [OSTI]

to act as both tumor suppressors and oncogenes in several different cancers, expression patterns of ten miRNAs (miR-15a, miR-16, miR-17-5p, miR-21, miR-29b, miR-125b, miR-145, miR-155, miR-181b, let-7f) known to be associated with human breast cancer were...

Boggs, Rene' Michelle

2008-10-10T23:59:59.000Z

284

Laminin Mediates Tissue-specific Gene Expression in Mammary Epithelia  

E-Print Network [OSTI]

417. Aumailley, M. , C. Battaglia, U. Mayer, D. Reinhardt,S. Conzelmann, P. Ekblom, C. Battaglia, M. Aumailley, and R.

Streuli, Charles H

2011-01-01T23:59:59.000Z

285

Prolonged effect of fluid flow stress on the proliferative activity of mesothelial cells after abrupt discontinuation of fluid streaming  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Late-onset peritoneal fibrosis leading to EPS remains to be elucidated. Black-Right-Pointing-Pointer Fluid streaming is a potent factor for peritoneal fibrosis in PD. Black-Right-Pointing-Pointer We focused on the prolonged effect of fluid streaming on mesothelial cell kinetics. Black-Right-Pointing-Pointer A history of fluid streaming exposure promoted mesothelial proliferative activity. Black-Right-Pointing-Pointer We have thus identified a potent new factor for late-onset peritoneal fibrosis. -- Abstract: Encapsulating peritoneal sclerosis (EPS) often develops after transfer to hemodialysis and transplantation. Both termination of peritoneal dialysis (PD) and transplantation-related factors are risks implicated in post-PD development of EPS, but the precise mechanism of this late-onset peritoneal fibrosis remains to be elucidated. We previously demonstrated that fluid flow stress induced mesothelial proliferation and epithelial-mesenchymal transition via mitogen-activated protein kinase (MAPK) signaling. Therefore, we speculated that the prolonged bioactive effect of fluid flow stress may affect mesothelial cell kinetics after cessation of fluid streaming. To investigate how long mesothelial cells stay under the bioactive effect brought on by fluid flow stress after removal of the stress, we initially cultured mesothelial cells under fluid flow stress and then cultured the cells under static conditions. Mesothelial cells exposed to fluid flow stress for a certain time showed significantly high proliferative activity compared with static conditions after stoppage of fluid streaming. The expression levels of protein phosphatase 2A, which dephosphorylates MAPK, in mesothelial cells changed with time and showed a biphasic pattern that was dependent on the duration of exposure to fluid flow stress. There were no differences in the fluid flow stress-related bioactive effects on mesothelial cells once a certain time had passed. The present findings show that fluid flow stress exerts a prolonged bioactive effect on mesothelial cells after termination of fluid streaming. These findings support the hypothesis that a history of PD for a certain period could serve as a trigger of EPS after stoppage of PD.

Aoki, Shigehisa, E-mail: aokis@cc.saga-u.ac.jp [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan); Ikeda, Satoshi [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan); Takezawa, Toshiaki [Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki (Japan)] [Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki (Japan); Kishi, Tomoya [Department of Internal Medicine, Saga University, Saga (Japan)] [Department of Internal Medicine, Saga University, Saga (Japan); Makino, Junichi [Makino Clinic, Saga (Japan)] [Makino Clinic, Saga (Japan); Uchihashi, Kazuyoshi; Matsunobu, Aki [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan); Noguchi, Mitsuru [Department of Urology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Urology, Faculty of Medicine, Saga University, Saga (Japan); Sugihara, Hajime [Department of Physical Therapy, International University of Health and Welfare, Fukuoka (Japan)] [Department of Physical Therapy, International University of Health and Welfare, Fukuoka (Japan); Toda, Shuji [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)] [Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga (Japan)

2011-12-16T23:59:59.000Z

286

A novel synthetic analog of militarin, MA-1 induces mitochondrial dependent apoptosis by ROS generation in human lung cancer cells  

SciTech Connect (OSTI)

A synthetic Militarin analog-1[(2R,3R,4R,5R)-1,6-bis(4-(2,4,4-trimethylpentan-2-yl)phenoxy) hexane-2,3,4,5-tetraol] is a novel derivative of constituents from Cordyceps militaris, which has been used to treat a variety of chronic diseases including inflammation, diabetes, hyperglycemia and cancers. Here, we report for the first time the synthesis of Militarin analog-1 (MA-1) and the apoptotic mechanism of MA-1 against human lung cancer cell lines. Treatment with MA-1 significantly inhibited the viability of 3 human lung cancer cell lines. The inhibition of viability and growth in MA-1-treated A549 cells with an IC{sub 50} of 5 ?M were mediated through apoptosis induction, as demonstrated by an increase in DNA fragmentation, sub-G{sub 0}/G{sub 1}-DNA fraction, nuclear condensation, and phosphatidylserine exposure. The apoptotic cell death caused mitochondrial membrane permeabilization through regulation of expression of the Bcl-2 family proteins, leading to cytochrome c release in a time-dependent manner. Subsequently, the final stage of apoptosis, activation of caspase-9/-3 and cleavage of poly (ADP ribose) polymerase, was induced. Furthermore, A549 lung cancer cells were more responsive to MA-1 than a bronchial epithelial cell line (BEAS-2B), involving the rapid generation of reactive oxygen species (ROS), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) activation. The pharmacological inhibition of ROS generation and JNK/p38 MAPK exhibited attenuated DNA fragmentation in MA-1-induced apoptosis. Oral administration of MA-1 also retarded growth of A549 orthotopic xenografts. In conclusion, the present study indicates that the new synthetic derivative MA-1 triggers mitochondrial apoptosis through ROS generation and regulation of MAPKs and may be a potent therapeutic agent against human lung cancer. - Highlights: • We report a novel synthesized derivative, militarin analog-1 (MA-1). • MA-1-induced cancer cell death was triggered by the ROS generation through MAPKs. • The MA-1-induced cell death was also modulated by the mitochondria-mediated pathway. • The apoptotic cancer cell death by MA-1 was also exhibited in orthotopic xenografts. • Our findings suggest MA-1 as a clinically useful agent for human lung cancer.

Yoon, Deok Hyo; Lim, Mi-Hee [Department of Biochemistry, Kangwon National University, Chuncheon 200-701 (Korea, Republic of); Lee, Yu Ran [Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747 (Korea, Republic of); Sung, Gi-Ho [Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 404-707 (Korea, Republic of); Lee, Tae-Ho [R and D Center, Dong-A Pharmaceutical Co, Ltd, Yongin 446-905 (Korea, Republic of); Jeon, Byeong Hwa [Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747 (Korea, Republic of); Cho, Jae Youl [Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Song, Won O. [Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824 (United States); Park, Haeil [College of Pharmacy, Kangwon National University, Chuncheon 200-701 (Korea, Republic of); Choi, Sunga, E-mail: sachoi@cnu.ac.kr [Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747 (Korea, Republic of); Kim, Tae Woong, E-mail: tawkim@kangwon.ac.kr [Department of Biochemistry, Kangwon National University, Chuncheon 200-701 (Korea, Republic of)

2013-12-15T23:59:59.000Z

287

An advanced fuel cell simulator  

E-Print Network [OSTI]

Fuel cell power generation systems provide a clean alternative to the conventional fossil fuel based systems. Fuel cell systems have a high e?ciency and use easily available hydrocarbons like methane. Moreover, since the by-product is water...

Acharya, Prabha Ramchandra

2005-11-01T23:59:59.000Z

288

Fuel Cell Projects Kickoff Meeting  

Broader source: Energy.gov (indexed) [DOE]

3:40 Aligned Carbon Nanotube-Based MEA and PEMFC D-J Liu, ANL 4:00 Light Weight Low Cost PEM Fuel Cell Stacks J. Wainright, CWRU 4:20 Adaptive Stack with Subdivided Cells for...

289

Crystalline Silicon Photovolatic Cell Basics  

Broader source: Energy.gov [DOE]

Crystalline silicon cells are made of silicon atoms connected to one another to form a crystal lattice. This lattice comprises the solid material that forms the photovoltaic (PV) cell's...

290

2009 Fuel Cell Market Report  

Fuel Cell Technologies Publication and Product Library (EERE)

Fuel cells are electrochemical devices that combine hydrogen and oxygen to produce electricity, water, and heat. Unlike batteries, fuel cells continuously generate electricity, as long as a source of

291

DOE Hydrogen & Fuel Cell Overview  

Broader source: Energy.gov (indexed) [DOE]

Natural Gas Power Heat + Cooling Electricity Cooling Natural Gas Natural Gas or Biogas Fuel Cell H Excess power generated by the fuel cell is fed to the grid National...

292

Fuel Cells - Current Technology | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cells - Current Technology Fuel Cells - Current Technology Today, fuel cells are being developed to power passenger vehicles, commercial buildings, homes, and even small...

293

Fabrication and Characterization of Organic Solar Cells  

E-Print Network [OSTI]

processable polymer photovoltaic cells by self?organization Photodiodes,  and  Photovoltaic  Cells.   Applied Physics F,  Heeger  AJ.   Polymer  Photovoltaic  Cells  ?  Enhanced 

Yengel, Emre

2010-01-01T23:59:59.000Z

294

Fabrication and Characterization of Organic Solar Cells  

E-Print Network [OSTI]

electrodes  for  dye? sensitized solar cells.  Nano solar cells and dye-sensitized solar cells. Figure 1-3 The

Yengel, Emre

2010-01-01T23:59:59.000Z

295

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

Applications of Microscale Microbial Fuel Cell SystemsApplications of Microscale Microbial Fuel Cell Systems Infrom the use of microscale microbial fuel cells is that of

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

296

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

Sediment microbial fuel cells demonstrating marine (left)Model of hydrogen fuel cell kinetic losses including5 FutureWork 5.1 Microfluidic Microbial Fuel Cell Continued

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

297

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

Model of hydrogen fuel cell kinetic losses includingschematic of typical hydrogen fuel cell performancephase factors on hydrogen fuel cell theoretical efficiency,

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

298

airway permeability role: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

present in epithelial cells from individuals without asthma a central role in allergic of individuals with asthma, this two-step process for IL-16 production by epithelial cells...

299

ameliorates interleukin 2-induced: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

present in epithelial cells from individuals without asthma a central role in allergic of individuals with asthma, this two-step process for IL-16 production by epithelial cells...

300

National Fuel Cell Research Center  

E-Print Network [OSTI]

National Fuel Cell Research Center www.nfcrc.uci.edu CONTROLS RESIDENTIAL FUEL CELL PHOTOVOLTAIC and efficiency, (3) RFC produces hydrogen, a flexible fuel that may be used for electricity, vehicles, heating fuel cells (RFC), we gain access to a new energy storage device that is both analogous to rechargeable

Mease, Kenneth D.

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Method for fabricating silicon cells  

DOE Patents [OSTI]

A process for making high-efficiency solar cells. This is accomplished by forming a diffusion junction and a passivating oxide layer in a single high-temperature process step. The invention includes the class of solar cells made using this process, including high-efficiency solar cells made using Czochralski-grown silicon.

Ruby, Douglas S. (Albuquerque, NM); Basore, Paul A. (Albuquerque, NM); Schubert, W. Kent (Albuquerque, NM)

1998-08-11T23:59:59.000Z

302

Fuel cell generator energy dissipator  

DOE Patents [OSTI]

An apparatus and method are disclosed for eliminating the chemical energy of fuel remaining in a fuel cell generator when the electrical power output of the fuel cell generator is terminated. During a generator shut down condition, electrically resistive elements are automatically connected across the fuel cell generator terminals in order to draw current, thereby depleting the fuel

Veyo, Stephen Emery (Murrysville, PA); Dederer, Jeffrey Todd (Valencia, PA); Gordon, John Thomas (Ambridge, PA); Shockling, Larry Anthony (Pittsburgh, PA)

2000-01-01T23:59:59.000Z

303

Method for fabricating silicon cells  

DOE Patents [OSTI]

A process is described for making high-efficiency solar cells. This is accomplished by forming a diffusion junction and a passivating oxide layer in a single high-temperature process step. The invention includes the class of solar cells made using this process, including high-efficiency solar cells made using Czochralski-grown silicon. 9 figs.

Ruby, D.S.; Basore, P.A.; Schubert, W.K.

1998-08-11T23:59:59.000Z

304

Microfluidic Fuel Cells Erik Kjeang  

E-Print Network [OSTI]

Microfluidic Fuel Cells by Erik Kjeang M.Sc., Umeå University, 2004 A Dissertation Submitted Supervisory Committee Microfluidic Fuel Cells by Erik Kjeang M.Sc., Umeå University, 2004 Supervisory University External Examiner Microfluidic fuel cell architectures are presented in this thesis. This work

Victoria, University of

305

Compact fuel cell  

DOE Patents [OSTI]

A novel electrochemical cell which may be a solid oxide fuel cell (SOFC) is disclosed where the cathodes (144, 140) may be exposed to the air and open to the ambient atmosphere without further housing. Current collector (145) extends through a first cathode on one side of a unit and over the unit through the cathode on the other side of the unit and is in electrical contact via lead (146) with housing unit (122 and 124). Electrical insulator (170) prevents electrical contact between two units. Fuel inlet manifold (134) allows fuel to communicate with internal space (138) between the anodes (154 and 156). Electrically insulating members (164 and 166) prevent the current collector from being in electrical contact with the anode.

Jacobson, Craig (Moraga, CA); DeJonghe, Lutgard C. (Lafayette, CA); Lu, Chun (Richland, WA)

2010-10-19T23:59:59.000Z

306

Electrocapturing flow cell  

DOE Patents [OSTI]

A flow cell for electrophoretically-assisted capturing analytes from a flow. The flow cell includes a specimen chamber, a first membrane, a second membrane, a first electrode chamber, and a second electrode chamber. The specimen chamber may have a sample inlet and a sample outlet. A first portion of the first membrane may be coupled to a first portion of the specimen chamber. A first portion of the second membrane may be coupled to a second portion of the specimen chamber. The first electrode chamber may be configured to accept a charge. A portion of the first electrode chamber may be coupled to a second portion of the first membrane. A second electrode chamber may be configured to accept an opposite charge. A portion of the second electrode chamber may be coupled to a second portion of the second membrane.

Morozov, Victor (Manassas, VA)

2011-04-05T23:59:59.000Z

307

Bilevel contact solar cells  

SciTech Connect (OSTI)

This patent describes a solar cell. It comprises a body of semiconductor material having at least one P/N junction therein, the body including a front face having no electrodes thereon, and a bilevel elevation back face having at least one P-doped region at a first level interdigitated with at least one N-doped region at a second level, wherein the at least one P-doped region and the at least one N-doped region partially overlap to form at least one compensated region; and a positive electrode contacting the at lease one P-doped region and a negative electrode contacting the at least one N-doped region, both electrodes contacting the solar cell on the back face.

Sinton, R.A.

1991-10-01T23:59:59.000Z

308

Broad spectrum solar cell  

DOE Patents [OSTI]

An alloy having a large band gap range is used in a multijunction solar cell to enhance utilization of the solar energy spectrum. In one embodiment, the alloy is In.sub.1-xGa.sub.xN having an energy bandgap range of approximately 0.7 eV to 3.4 eV, providing a good match to the solar energy spectrum. Multiple junctions having different bandgaps are stacked to form a solar cell. Each junction may have different bandgaps (realized by varying the alloy composition), and therefore be responsive to different parts of the spectrum. The junctions are stacked in such a manner that some bands of light pass through upper junctions to lower junctions that are responsive to such bands.

Walukiewicz, Wladyslaw (Kensington, CA); Yu, Kin Man (Lafayette, CA); Wu, Junqiao (Richmond, CA); Schaff, William J. (Ithaca, NY)

2007-05-15T23:59:59.000Z

309

Fuel cell CO sensor  

DOE Patents [OSTI]

The CO concentration in the H.sub.2 feed stream to a PEM fuel cell stack is monitored by measuring current and/or voltage behavior patterns from a PEM-probe communicating with the reformate feed stream. Pattern recognition software may be used to compare the current and voltage patterns from the PEM-probe to current and voltage telltale outputs determined from a reference cell similar to the PEM-probe and operated under controlled conditions over a wide range of CO concentrations in the H.sub.2 fuel stream. A CO sensor includes the PEM-probe, an electrical discharge circuit for discharging the PEM-probe to monitor the CO concentration, and an electrical purging circuit to intermittently raise the anode potential of the PEM-probe's anode to at least about 0.8 V (RHE) to electrochemically oxidize any CO adsorbed on the probe's anode catalyst.

Grot, Stephen Andreas (Rochester, NY); Meltser, Mark Alexander (Pittsford, NY); Gutowski, Stanley (Pittsford, NY); Neutzler, Jay Kevin (Rochester, NY); Borup, Rodney Lynn (East Rochester, NY); Weisbrod, Kirk (Los Alamos, NM)

1999-12-14T23:59:59.000Z

310

Aluminum reduction cell electrode  

DOE Patents [OSTI]

The invention is directed to an anode-cathode structure for an electrolytic cell for the reduction of alumina wherein the structure is comprised of a carbon anode assembly which straddles a wedge-shaped refractory hard metal cathode assembly having steeply sloped cathodic surfaces, each cathodic surface being paired in essentially parallel planar relationship with an anode surface. The anode-cathode structure not only takes into account the structural weakness of refractory hard metal materials but also permits the changing of the RHM assembly during operation of the cell. Further, the anode-cathode structure enhances the removal of anode gas from the interpolar gap between the anode and cathode surfaces. 10 figs.

Payne, J.R.

1983-09-20T23:59:59.000Z

311

Fuel Cell Handbook, Fifth Edition  

SciTech Connect (OSTI)

Progress continues in fuel cell technology since the previous edition of the Fuel Cell Handbook was published in November 1998. Uppermost, polymer electrolyte fuel cells, molten carbonate fuel cells, and solid oxide fuel cells have been demonstrated at commercial size in power plants. The previously demonstrated phosphoric acid fuel cells have entered the marketplace with more than 220 power plants delivered. Highlighting this commercial entry, the phosphoric acid power plant fleet has demonstrated 95+% availability and several units have passed 40,000 hours of operation. One unit has operated over 49,000 hours. Early expectations of very low emissions and relatively high efficiencies have been met in power plants with each type of fuel cell. Fuel flexibility has been demonstrated using natural gas, propane, landfill gas, anaerobic digester gas, military logistic fuels, and coal gas, greatly expanding market opportunities. Transportation markets worldwide have shown remarkable interest in fuel cells; nearly every major vehicle manufacturer in the U.S., Europe, and the Far East is supporting development. This Handbook provides a foundation in fuel cells for persons wanting a better understanding of the technology, its benefits, and the systems issues that influence its application. Trends in technology are discussed, including next-generation concepts that promise ultrahigh efficiency and low cost, while providing exceptionally clean power plant systems. Section 1 summarizes fuel cell progress since the last edition and includes existing power plant nameplate data. Section 2 addresses the thermodynamics of fuel cells to provide an understanding of fuel cell operation at two levels (basic and advanced). Sections 3 through 8 describe the six major fuel cell types and their performance based on cell operating conditions. Alkaline and intermediate solid state fuel cells were added to this edition of the Handbook. New information indicates that manufacturers have stayed with proven cell designs, focusing instead on advancing the system surrounding the fuel cell to lower life cycle costs. Section 9, Fuel Cell Systems, has been significantly revised to characterize near-term and next-generation fuel cell power plant systems at a conceptual level of detail. Section 10 provides examples of practical fuel cell system calculations. A list of fuel cell URLs is included in the Appendix. A new index assists the reader in locating specific information quickly.

Energy and Environmental Solutions

2000-10-31T23:59:59.000Z

312

Cell culture compositions  

DOE Patents [OSTI]

The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6 (SEQ ID NO:1 encodes the full length endoglucanase; SEQ ID NO:4 encodes the mature form), and the corresponding endoglucanase VI amino acid sequence ("EGVI"; SEQ ID NO:3 is the signal sequence; SEQ ID NO:2 is the mature sequence). The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yiao, Jian

2014-03-18T23:59:59.000Z

313

Miniature ceramic fuel cell  

DOE Patents [OSTI]

A miniature power source assembly capable of providing portable electricity is provided. A preferred embodiment of the power source assembly employing a fuel tank, fuel pump and control, air pump, heat management system, power chamber, power conditioning and power storage. The power chamber utilizes a ceramic fuel cell to produce the electricity. Incoming hydro carbon fuel is automatically reformed within the power chamber. Electrochemical combustion of hydrogen then produces electricity.

Lessing, Paul A. (Idaho Falls, ID); Zuppero, Anthony C. (Idaho Falls, ID)

1997-06-24T23:59:59.000Z

314

Rapidly refuelable fuel cell  

DOE Patents [OSTI]

A rapidly refuelable dual cell of an electrochemical type is described wherein a single anode cooperates with two cathodes and wherein the anode has a fixed position and the cathodes are urged toward opposite faces of the anodes at constant and uniform force. The associated cathodes are automatically retractable to permit the consumed anode remains to be removed from the housing and a new anode inserted between the two cathodes.

Joy, R.W.

1982-09-20T23:59:59.000Z

315

Fuel cell system combustor  

DOE Patents [OSTI]

A fuel cell system including a fuel reformer heated by a catalytic combustor fired by anode and cathode effluents. The combustor includes a turbulator section at its input end for intimately mixing the anode and cathode effluents before they contact the combustors primary catalyst bed. The turbulator comprises at least one porous bed of mixing media that provides a tortuous path therethrough for creating turbulent flow and intimate mixing of the anode and cathode effluents therein.

Pettit, William Henry (Rochester, NY)

2001-01-01T23:59:59.000Z

316

Massively Parallel Microfluidic Cell-Pairing Platform for the Statistical Study of Immunological Cell-Cell Interactions  

E-Print Network [OSTI]

Variability in cell-cell interactions is ubiquitous and particularly relevant for the immune system, where the reliability of cell-cell interactions is critical for the prevention of disease. This variability is poorly ...

Hoehl, Melanie Margarete

317

Although leukocyte populations, including macro phages, dendritic cells (DCs), B cells and T cells, can  

E-Print Network [OSTI]

cytotoxic T lymphocytes that recognize the tissue of the recipient as foreign. GVHD varies markedly cells. Regulatory immune cells in transplantation Kathryn J. Wood, Andrew Bushell and Joanna Hester

Cai, Long

318

Double interconnection fuel cell array  

DOE Patents [OSTI]

A fuel cell array is made, containing number of tubular, elongated fuel cells which are placed next to each other in rows (A, B, C, D), where each cell contains inner electrodes and outer electrodes, with solid electrolyte between the electrodes, where the electrolyte and outer electrode are discontinuous, having two portions, and providing at least two opposed discontinuities which contain at least two oppositely opposed interconnections contacting the inner electrode, each cell having only three metallic felt electrical connectors which contact surrounding cells, where each row is electrically connected to the other. 5 figures.

Draper, R.; Zymboly, G.E.

1993-12-28T23:59:59.000Z

319

Self-humidified proton exchange membrane fuel cells: Operation of larger cells and fuel cell stacks  

SciTech Connect (OSTI)

The PEM fuel cell is promising as the power source for use in mobile and stationary applications primarily because of its high power density, all solid components, and simplicity of operation. For wide acceptability of this power source, its cost has to be competitive with the presently available energy sources. The fuel cell requires continuous humidification during operation as a power source. The humidification unit however, increases fuel cell volume, weight, and therefore decreases its overall power density. Great advantages in terms of further fuel cell simplification can be achieved if the humidification process can be eliminated or minimized. In addition, cost reductions are associated with the case of manufacturing and operation. At BCS Technology we have developed a technology of self-humidified operation of PEM fuel cells based on the mass balance of the reactants and products and the ability of membrane electrode assembly (MEA) to retain water necessary for humidification under the cell operating conditions. The reactants enter the fuel cell chambers without carrying any form of water, whether in liquid or vapor form. Basic principles of self-humidified operation of fuel cells as practiced by BCS Technology, Inc. have been presented previously in literature. Here, we report the operation of larger self-humidified single cells and fuel cell stacks. Fuel cells of areas Up to 100 cm{sup 2} have been operated. We also show the self-humidified operation of fuel cell stacks of 50 and 100 cm{sup 2} electrode areas.

Dhar, H.P.; Lee, J.H.; Lewinski, K.A. [BCS Technology, Inc., Bryan, TX (United States)

1996-12-31T23:59:59.000Z

320

E-Print Network 3.0 - apical cl- channels Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

arrows show apical and basal surfaces, respectively... Developmental Cell Article Apical Secretion in Epithelial Tubes of the Drosophila Embryo... *Correspondence:...

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Nickel coated aluminum battery cell tabs  

DOE Patents [OSTI]

A battery cell tab is described. The battery cell tab is anodized on one end and has a metal coating on the other end. Battery cells and methods of making battery cell tabs are also described.

Bucchi, Robert S.; Casoli, Daniel J.; Campbell, Kathleen M.; Nicotina, Joseph

2014-07-29T23:59:59.000Z

322

EE580 Solar Cells Todd J. Kaiser  

E-Print Network [OSTI]

7/21/2010 1 EE580 ­ Solar Cells Todd J. Kaiser · Lecture 08 · Solar Cell Characterization 1Montana State University: Solar Cells Lecture 8: Characterization Solar Cell Operation n Emitter p Base Rear completing the circuit 2Montana State University: Solar Cells Lecture 8: Characterization Solar Cell

Kaiser, Todd J.

323

FUEL CELL TECHNOLOGIES PROGRAM Hydrogen and Fuel  

E-Print Network [OSTI]

collectors. In a Polymer Electrolyte Membrane (PEM) fuel cell, which is widely regarded as the most promisingFUEL CELL TECHNOLOGIES PROGRAM Hydrogen and Fuel Cell Technologies Program: Fuel Cells Fuel Cells -- is the key to making it happen. Stationary fuel cells can be used for backup power, power for remote loca

324

Carbonate fuel cell anodes  

DOE Patents [OSTI]

A molten alkali metal carbonates fuel cell porous anode of lithium ferrite and a metal or metal alloy of nickel, cobalt, nickel/iron, cobalt/iron, nickel/iron/aluminum, cobalt/iron/aluminum and mixtures thereof wherein the total iron content including ferrite and iron of the composite is about 25 to about 80 percent, based upon the total anode, provided aluminum when present is less than about 5 weight percent of the anode. A process is described for production of the lithium ferrite containing anode by slipcasting.

Donado, R.A.; Hrdina, K.E.; Remick, R.J.

1993-04-27T23:59:59.000Z

325

Carbonate fuel cell anodes  

DOE Patents [OSTI]

A molten alkali metal carbonates fuel cell porous anode of lithium ferrite and a metal or metal alloy of nickel, cobalt, nickel/iron, cobalt/iron, nickel/iron/aluminum, cobalt/iron/aluminum and mixtures thereof wherein the total iron content including ferrite and iron of the composite is about 25 to about 80 percent, based upon the total anode, provided aluminum when present is less than about 5 weight percent of the anode. A process for production of the lithium ferrite containing anode by slipcasting.

Donado, Rafael A. (Chicago, IL); Hrdina, Kenneth E. (Glenview, IL); Remick, Robert J. (Bolingbrook, IL)

1993-01-01T23:59:59.000Z

326

Monolithic tandem solar cell  

DOE Patents [OSTI]

A single-crystal, monolithic, tandem, photovoltaic solar cell is described which includes (a) an InP substrate having upper and lower surfaces, (b) a first photoactive subcell on the upper surface of the InP substrate, (c) a second photoactive subcell on the first subcell; and (d) an optically transparent prismatic cover layer over the second subcell. The first photoactive subcell is GaInAsP of defined composition. The second subcell is InP. The two subcells are lattice matched.

Wanlass, Mark W. (Golden, CO)

1994-01-01T23:59:59.000Z

327

Fuel cell having electrolyte  

DOE Patents [OSTI]

A fuel cell having an electrolyte control volume includes a pair of porous opposed electrodes. A maxtrix is positioned between the pair of electrodes for containing an electrolyte. A first layer of backing paper is positioned adjacent to one of the electrodes. A portion of the paper is substantially previous to the acceptance of the electrolyte so as to absorb electrolyte when there is an excess in the matrix and to desorb electrolyte when there is a shortage in the matrix. A second layer of backing paper is positioned adjacent to the first layer of paper and is substantially impervious to the acceptance of electrolyte.

Wright, Maynard K. (Bethel Park, PA)

1989-01-01T23:59:59.000Z

328

Monolithic tandem solar cell  

DOE Patents [OSTI]

A single-crystal, monolithic, tandem, photovoltaic solar cell is described which includes (a) an InP substrate having upper and lower surfaces, (b) a first photoactive subcell on the upper surface of the InP substrate, (c) a second photoactive subcell on the first subcell; and (d) an optically transparent prismatic cover layer over the second subcell. The first photoactive subcell is GaInAsP of defined composition. The second subcell is InP. The two subcells are lattice matched. 9 figs.

Wanlass, M.W.

1994-06-21T23:59:59.000Z

329

Fuel Cell Technologies Overview  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:YearRound-UpHeatMulti-Dimensional ElectricalEnergy Frozen TelescopeRenewable 0 0 A N09Fuel Cell

330

Automotive Fuel Cell Corporation  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth (AOD)ProductssondeadjustsondeadjustAboutScience Program Cumulus Humilis, 2014AutomatedAutomotive Fuel Cell

331

Mammalian Cell Culture | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas Conchas recovery challenge fund Las ConchasTrail5,722,326 SiteMammalian Cell Culture

332

Role of hypoxia and hypoxia induced factors in the development of breast cancer brain metastasis  

E-Print Network [OSTI]

Here we studied the role of hypoxia and hypoxia-induced factors in the development of breast cancer brain metastasis by using ENU1564, a carcinogen-induced mammary adenocarcinoma cell line. We detected hypoxia noninvasively by using a novel...

Lungu, Gina Florentina

2009-05-15T23:59:59.000Z

333

Flexible method for monitoring fuel cell voltage  

DOE Patents [OSTI]

A method for equalizing the measured voltage of each cluster in a fuel cell stack wherein at least one of the clusters has a different number of cells than the identical number of cells in the remaining clusters by creating a pseudo voltage for the different cell numbered cluster. The average cell voltage of the all of the cells in the fuel cell stack is calculated and multiplied by a constant equal to the difference in the number of cells in the identical cell clusters and the number of cells in the different numbered cell cluster. The resultant product is added to the actual voltage measured across the different numbered cell cluster to create a pseudo voltage which is equivalent in cell number to the number of cells in the other identical numbered cell clusters.

Mowery, Kenneth D. (Noblesville, IN); Ripley, Eugene V. (Russiaville, IN)

2002-01-01T23:59:59.000Z

334

DNDO Analysis Cell Concept  

SciTech Connect (OSTI)

The Domestic Nuclear Detection Office (DNDO) has a mission of implementing rad/nuc interdiction capabilities for a managed and coordinated response to threats, integration of federal nuclear forensics programs, and coordinating the development of the global nuclear detection and reporting architecture. In the process of executing this mission, DNDO has generated substantial information, data, technical results, operational workflows and analytical tools. The effective utilization of these resources is an overarching goal of the organization. After nearly a decade of performing work, DNDO faces a challenge in capitalizing on the large amount of data, reports, processes, tools, and people. As new work is being planned, managers and researchers need to have an understating of what information has been collected, what tools are available, the collaborations which can be utilized to propel the work forward, processes to plan and execute, and how to present conclusions and results that can assist the government in making decisions. This type of challenge can be met through the use of a series of organized and connected elements which form a broader structure (cell) that promotes cross utilization of elements such that they can be tailored (analyzed) to fit the context of the problem to be solved. The development of an analysis cell for DNDO will address the challenges of utilizing existing elements, identifying gaps, annually reporting the performance of rad/nuc interdiction instrumentation, and planning the execution of future work.

Pagh, Richard T.; Dimmerling, Paul J.; Guillen, Zoe C.; Hoyt, Joel R.; Jarman, Kenneth D.; Kernan, Warnick J.; Reichmuth, Barbara A.; Rohlfing, Kerrie S.; Schweppe, John E.; Sego, Landon H.; Shergur, Jason M.; Siciliano, Edward R.; Woodring, Mitchell L.

2014-03-12T23:59:59.000Z

335

Hybrid Fuel Cell Technology Overview  

SciTech Connect (OSTI)

For the purpose of this STI product and unless otherwise stated, hybrid fuel cell systems are power generation systems in which a high temperature fuel cell is combined with another power generating technology. The resulting system exhibits a synergism in which the combination performs with an efficiency far greater than can be provided by either system alone. Hybrid fuel cell designs under development include fuel cell with gas turbine, fuel cell with reciprocating (piston) engine, and designs that combine different fuel cell technologies. Hybrid systems have been extensively analyzed and studied over the past five years by the Department of Energy (DOE), industry, and others. These efforts have revealed that this combination is capable of providing remarkably high efficiencies. This attribute, combined with an inherent low level of pollutant emission, suggests that hybrid systems are likely to serve as the next generation of advanced power generation systems.

None available

2001-05-31T23:59:59.000Z

336

Corrosion resistant PEM fuel cell  

DOE Patents [OSTI]

The present invention contemplates a PEM fuel cell having electrical contact elements (including bipolar plates/septums) comprising a titanium nitride coated light weight metal (e.g., Al or Ti) core, having a passivating, protective metal layer intermediate the core and the titanium nitride. The protective layer forms a barrier to further oxidation/corrosion when exposed to the fuel cell`s operating environment. Stainless steels rich in Cr, Ni, and Mo are particularly effective protective interlayers. 6 figs.

Li, Y.; Meng, W.J.; Swathirajan, S.; Harris, S.J.; Doll, G.L.

1997-04-29T23:59:59.000Z

337

Solar cell module lamination process  

DOE Patents [OSTI]

A solar cell module lamination process using fluoropolymers to provide protection from adverse environmental conditions and thus enable more extended use of solar cells, particularly in space applications. A laminate of fluoropolymer material provides a hermetically sealed solar cell module structure that is flexible and very durable. The laminate is virtually chemically inert, highly transmissive in the visible spectrum, dimensionally stable at temperatures up to about 200.degree. C. highly abrasion resistant, and exhibits very little ultra-violet degradation.

Carey, Paul G. (Mountain View, CA); Thompson, Jesse B. (Brentwood, CA); Aceves, Randy C. (Tracy, CA)

2002-01-01T23:59:59.000Z

338

Cell signalling and phospholipid metabolism  

SciTech Connect (OSTI)

These studies explored whether phosphoinositide (PI) has a role in plants analogous to its role in animal cells. Although no parallel activity of PI in signal transduction was found in plant cells, activity of inositol phospholipid kinase was found to be modulated by light and by cell wall degrading enzymes. These studies indicate a major role for inositol phospholipids in plant growth and development as membrane effectors but not as a source of second messengers.

Boss, W.F.

1990-01-01T23:59:59.000Z

339

Efficiency measurements of TPV cells  

SciTech Connect (OSTI)

An apparatus for measuring TPV cell efficiencies at different radiation intensities and for different graybody emitter temperatures has been constructed. The apparatus has been used for measuring V-I characteristics, efficiencies and fill factors for several InGaAs TPV cells. Measured results are used to determine how cells may function together with edge filters, and those results are compared with theory. {copyright} {ital 1997 American Institute of Physics.}

Broman, L.; Jarefors, K. [Solar Energy Research Center, Hoegskolan Dalarna, S-781 88 Borlaenge (Sweden); Marks, J. [Department of Operational Efficiency, Swedish University of Agricultural Sciences, Box 7060, S-750 07 Uppsala (Sweden); Wanlass, M. [National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, Colorado 80401-3393 (United States of America)

1997-03-01T23:59:59.000Z

340

Fuel cell gas management system  

DOE Patents [OSTI]

A fuel cell gas management system including a cathode humidification system for transferring latent and sensible heat from an exhaust stream to the cathode inlet stream of the fuel cell; an anode humidity retention system for maintaining the total enthalpy of the anode stream exiting the fuel cell equal to the total enthalpy of the anode inlet stream; and a cooling water management system having segregated deionized water and cooling water loops interconnected by means of a brazed plate heat exchanger.

DuBose, Ronald Arthur (Marietta, GA)

2000-01-11T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

CLIMATE CHANGE FUEL CELL PROGRAM  

SciTech Connect (OSTI)

This report discusses the first year of operation of a fuel cell power plant located at the Sheraton Edison Hotel, Edison, New Jersey. PPL EnergyPlus, LLC installed the plant under a contract with the Starwood Hotels & Resorts Worldwide, Inc. A DFC{reg_sign}300 fuel cell, manufactured by FuelCell Energy, Inc. of Danbury, CT was selected for the project. The fuel cell successfully operated from June 2003 to May 2004. This report discusses the performance of the plant during this period.

Steven A. Gabrielle

2004-12-03T23:59:59.000Z

342

Multi-cell storage battery  

DOE Patents [OSTI]

A multi-cell storage battery, in particular to a lithium storage battery, which contains a temperature control device and in which groups of one or more individual cells arranged alongside one another are separated from one another by a thermally insulating solid layer whose coefficient of thermal conductivity lies between 0.01 and 0.2 W/(m*K), the thermal resistance of the solid layer being greater by at least a factor .lambda. than the thermal resistance of the individual cell. The individual cell is connected, at least in a region free of insulating material, to a heat exchanger, the thermal resistance of the heat exchanger in the direction toward the neighboring cell being selected to be greater by at least a factor .lambda. than the thermal resistance of the individual cell and, in addition, the thermal resistance of the heat exchanger toward the temperature control medium being selected to be smaller by at least a factor of about 10 than the thermal resistance of the individual cell, and .lambda. being the ratio of the energy content of the individual cell to the amount of energy that is needed to trigger a thermally induced cell failure at a defined upper operating temperature limit.

Brohm, Thomas (Hattersheim, DE); Bottcher, Friedhelm (Kelkheim, DE)

2000-01-01T23:59:59.000Z

343

Thermal Management of Solar Cells.  

E-Print Network [OSTI]

??The focus on solar cells as a source of photovoltaic energy is rapidly increasing nowadays. The amount of sun's energy entering earth surface in one… (more)

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

344

Manufacturing Fuel Cell Manhattan Project  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Chief Scientist. There, he was responsible for proton exchange membrane (PEM) fuel cell technology assessment and advanced development, as well as technical initiatives within...

345

Thermal Management of Solar Cells  

E-Print Network [OSTI]

ratio of the solar cell output power to the incident lightpower to operate the fan. Natural cooling is preferred for solar

Saadah, Mohammed Ahmed

2013-01-01T23:59:59.000Z

346

Microfluidics for fuel cell applications.  

E-Print Network [OSTI]

??In this work, a microfluidics approach is applied to two fuel cell related projects; the study of deformation and contact angle hysteresis on water invasion… (more)

Stewart, Ian

2011-01-01T23:59:59.000Z

347

Air Liquide - Biogas & Fuel Cells  

Broader source: Energy.gov (indexed) [DOE]

Liquide - Biogas & Fuel Cells Hydrogen Energy Biogas Upgrading Technology 12 June 2012 Charlie.Anderson@airliquide.com 2 Air Liquide, world leader in gases for industry,...

348

T cells stimulate catabolic gene expression by the stromal cells from giant cell tumor of bone  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Two T cell lines stimulate PTHrP, RANKL, MMP13 gene expression in GCT cell cultures. Black-Right-Pointing-Pointer CD40 expressed by stromal cells; CD40L detected in whole tumor but not cultures. Black-Right-Pointing-Pointer Effect of CD40L treatment on GCT cells increased PTHrP and MMP13 gene expression. Black-Right-Pointing-Pointer PTHrP treatment increased MMP13 expression, while inhibition decreased expression. Black-Right-Pointing-Pointer T cells may stimulate GCT stromal cells and promote the osteolysis of the tumor. -- Abstract: The factors that promote the localized bone resorption by giant cell tumor of bone (GCT) are not fully understood. We investigated whether T cells could contribute to bone resorption by stimulating expression of genes for parathyroid hormone-related protein (PTHrP), matrix metalloproteinase (MMP)-13, and the receptor activator of nuclear-factor {kappa}B ligand (RANKL). Two cell lines, Jurkat clone E6-1 and D1.1, were co-cultured with isolated GCT stromal cells. Real-time PCR analyses demonstrated a significant increase of all three genes following 48 h incubation, and PTHrP and MMP-13 gene expression was also increased at 24 h. Further, we examined the expression of CD40 ligand (CD40L), a protein expressed by activated T cells, and its receptor, CD40, in GCT. Immunohistochemistry results revealed expression of the CD40 receptor in both the stromal cells and giant cells of the tumor. RNA collected from whole GCT tissues showed expression of CD40LG, which was absent in cultured stromal cells, and suggests that CD40L is expressed within GCT. Stimulation of GCT stromal cells with CD40L significantly increased expression of the PTHrP and MMP-13 genes. Moreover, we show that inhibition of PTHrP with neutralizing antibodies significantly decreased MMP13 expression by the stromal cells compared to IgG-matched controls, whereas stimulation with PTHrP (1-34) increased MMP-13 gene expression. These results suggest that T cells may potentiate the catabolic effect of GCT.

Cowan, Robert W. [Department of Pathology and Molecular Medicine, McMaster University, 1280 Main St. W., Hamilton, ON, Canada L8S 4L8 (Canada) [Department of Pathology and Molecular Medicine, McMaster University, 1280 Main St. W., Hamilton, ON, Canada L8S 4L8 (Canada); Juravinski Cancer Centre, 699 Concession St., Hamilton, ON, Canada L8V 5C2 (Canada); Ghert, Michelle [Juravinski Cancer Centre, 699 Concession St., Hamilton, ON, Canada L8V 5C2 (Canada) [Juravinski Cancer Centre, 699 Concession St., Hamilton, ON, Canada L8V 5C2 (Canada); Department of Surgery, McMaster University, 1280 Main St. W., Hamilton, ON, Canada L8S 4L8 (Canada); Singh, Gurmit, E-mail: gurmit.singh@jcc.hhsc.ca [Department of Pathology and Molecular Medicine, McMaster University, 1280 Main St. W., Hamilton, ON, Canada L8S 4L8 (Canada) [Department of Pathology and Molecular Medicine, McMaster University, 1280 Main St. W., Hamilton, ON, Canada L8S 4L8 (Canada); Juravinski Cancer Centre, 699 Concession St., Hamilton, ON, Canada L8V 5C2 (Canada)

2012-03-23T23:59:59.000Z

349

The Business Case for Fuel Cells 2012 America's Partner in Power  

E-Print Network [OSTI]

................................................................................................................... 5 Fuel Cells + Biogas...

350

Fuel Cell Hybrid Bus Lands at Hickam AFB: Hydrogen Fuel Cell...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Hybrid Bus Lands at Hickam AFB: Hydrogen Fuel Cell & Infrastructure Technologies Program, Fuel Cell Bus Demonstration Project (Fact Sheet) Fuel Cell Hybrid Bus Lands at Hickam AFB:...

351

Optimization of Fuel Cell System Operating Conditions for Fuel Cell Vehicles  

E-Print Network [OSTI]

An Indirect Methanol Pem Fuel Cell System, SAE 2001, (paperof automotive PEM fuel cell stacks, SAE 2000 (paper numberParasitic Loads in Fuel Cell Vehicles, International Journal

Zhao, Hengbing; Burke, Andy

2008-01-01T23:59:59.000Z

352

Optimization of Fuel Cell System Operating Conditions for Fuel Cell Vehicles  

E-Print Network [OSTI]

simulation tool for hydrogen fuel cell vehicles, Journal ofApplication on Direct Hydrogen Fuel Cell Vehicles, 2008. Acsystem for direct hydrogen fuel cell vehicles Fig. 3 Driver

Zhao, Hengbing; Burke, Andy

2008-01-01T23:59:59.000Z

353

Microbial fuel cells  

DOE Patents [OSTI]

A microbial fuel cell includes an anode compartment with an anode and an anode biocatalyst and a cathode compartment with a cathode and a cathode biocatalyst, with a membrane positioned between the anode compartment and the cathode compartment, and an electrical pathway between the anode and the cathode. The anode biocatalyst is capable of catalyzing oxidation of an organic substance, and the cathode biocatalyst is capable of catalyzing reduction of an inorganic substance. The reduced organic substance can form a precipitate, thereby removing the inorganic substance from solution. In some cases, the anode biocatalyst is capable of catalyzing oxidation of an inorganic substance, and the cathode biocatalyst is capable of catalyzing reduction of an organic or inorganic substance.

Nealson, Kenneth H; Pirbazari, Massoud; Hsu, Lewis

2013-04-09T23:59:59.000Z

354

PEM fuel cell degradation  

SciTech Connect (OSTI)

The durability of PEM fuel cells is a major barrier to the commercialization of these systems for stationary and transportation power applications. While significant progress has been made in understanding degradation mechanisms and improving materials, further improvements in durability are required to meet commercialization targets. Catalyst and electrode durability remains a primary degradation mode, with much work reported on understanding how the catalyst and electrode structure degrades. Accelerated Stress Tests (ASTs) are used to rapidly evaluate component degradation, however the results are sometimes easy, and other times difficult to correlate. Tests that were developed to accelerate degradation of single components are shown to also affect other component's degradation modes. Non-ideal examples of this include ASTs examining catalyst degradation performances losses due to catalyst degradation do not always well correlate with catalyst surface area and also lead to losses in mass transport.

Borup, Rodney L [Los Alamos National Laboratory; Mukundan, Rangachary [Los Alamos National Laboratory

2010-01-01T23:59:59.000Z

355

Photovoltaic cell assembly  

DOE Patents [OSTI]

A photovoltaic assembly for converting high intensity solar radiation into lectrical energy in which a solar cell is separated from a heat sink by a thin layer of a composite material which has excellent dielectric properties and good thermal conductivity. This composite material is a thin film of porous Al.sub.2 O.sub.3 in which the pores have been substantially filled with an electrophoretically-deposited layer of a styrene-acrylate resin. This composite provides electrical breakdown strengths greater than that of a layer consisting essentially of Al.sub.2 O.sub.3 and has a higher thermal conductivity than a layer of styrene-acrylate alone.

Beavis, Leonard C. (Albuquerque, NM); Panitz, Janda K. G. (Edgewood, NM); Sharp, Donald J. (Albuquerque, NM)

1990-01-01T23:59:59.000Z

356

Cell-to-cell communication and cellular environment alter the somatostatin status of delta cells  

SciTech Connect (OSTI)

Research highlights: {yields} TGP52 cells display enhanced functionality in pseudoislet form. {yields} Somatostatin content was reduced, but secretion increased in high glucose conditions. {yields} Cellular interactions and environment alter the somatostatin status of TGP52 cells. -- Abstract: Introduction: Somatostatin, released from pancreatic delta cells, is a potent paracrine inhibitor of insulin and glucagon secretion. Islet cellular interactions and glucose homeostasis are essential to maintain normal patterns of insulin secretion. However, the importance of cell-to-cell communication and cellular environment in the regulation of somatostatin release remains unclear. Methods: This study employed the somatostatin-secreting TGP52 cell line maintained in DMEM:F12 (17.5 mM glucose) or DMEM (25 mM glucose) culture media. The effect of pseudoislet formation and culture medium on somatostatin content and release in response to a variety of stimuli was measured by somatostatin EIA. In addition, the effect of pseudoislet formation on cellular viability (MTT and LDH assays) and proliferation (BrdU ELISA) was determined. Results: TGP52 cells readily formed pseudoislets and showed enhanced functionality in three-dimensional form with increased E-cadherin expression irrespective of the culture environment used. However, culture in DMEM decreased cellular somatostatin content (P < 0.01) and increased somatostatin secretion in response to a variety of stimuli including arginine, calcium and PMA (P < 0.001) when compared with cells grown in DMEM:F12. Configuration of TGP52 cells as pseudoislets reduced the proliferative rate and increased cellular cytotoxicity irrespective of culture medium used. Conclusions: Somatostatin secretion is greatly facilitated by cell-to-cell interactions and E-cadherin expression. Cellular environment and extracellular glucose also significantly influence the function of delta cells.

Kelly, Catriona, E-mail: catriona.kelly@qub.ac.uk [SAAD Centre for Pharmacy and Diabetes, School of Biomedical Sciences, University of Ulster, Coleraine (United Kingdom)] [SAAD Centre for Pharmacy and Diabetes, School of Biomedical Sciences, University of Ulster, Coleraine (United Kingdom); Flatt, Peter R.; McClenaghan, Neville H. [SAAD Centre for Pharmacy and Diabetes, School of Biomedical Sciences, University of Ulster, Coleraine (United Kingdom)] [SAAD Centre for Pharmacy and Diabetes, School of Biomedical Sciences, University of Ulster, Coleraine (United Kingdom)

2010-08-20T23:59:59.000Z

357

Microfluidic platform for the study of intercellular communication via soluble factor-cell and cell-cell  

E-Print Network [OSTI]

Microfluidic platform for the study of intercellular communication via soluble factor-cell and cell are available today. Here, we report the design and validation of a microfluidic platform that enables (i) soluble molecule-cell and/or (ii) cell-cell paracrine signaling. In the microfluidic platform, multiple

Kenis, Paul J. A.

358

Additive estrogenic effects of mixtures of frequently used UV filters on pS2-gene transcription in MCF-7 cells  

SciTech Connect (OSTI)

In order to protect consumers from ultraviolet (UV) radiation and enhance light stability of the product, three to eight UV filters are usually added to consumer sunscreen products. High lipophilicity of the UV filters has been shown to cause bioaccumulation in fish and humans, leading to environmental levels of UV filters that are similar to those of PCBs and DDT. In this paper, estrogen-regulated pS2 gene transcription in the human mammary tumor cell line MCF-7 was used as a measure of estrogenicity of four individual UV filters. Since humans are exposed to more than one UV filter at a time, an equipotent binary mixture of 2-hydroxy-4-methoxy-benzophenone (BP-3) and its metabolite 2,4-dihydroxy benzophenone (BP-1), as well as an equipotent multi-component mixture of BP-1, BP-3, octyl methoxy cinnamate (OMC) and 3-(4-methylbenzylidene) camphor (4-MBC), were also evaluated for their ability to induce pS2 gene transcription in order to examine additivity. An estrogen receptor-mediated mechanism of action was expected for all UV filters. Therefore, our null-hypothesis was that combined estrogenic responses, measured as increased pS2 gene transcription in MCF-7 cells after exposure to mixtures of UV filters, are additive, according to a concentration-addition model. Not all UV filters produced a full concentration-response curve within the concentration range tested (100 nM-1 {mu}M). Therefore, instead of using EC{sub 50} values for comparison, the concentration at which each compound caused a 50% increase of basal pS2 gene transcription was defined as the C50 value for that compound and used to calculate relative potencies. For comparison, the EC{sub 50} value of a compound is the concentration at which the compound elicits an effect that is 50% of its maximal effect. Individual UV filters increased pS2 gene transcription concentration-dependently with C50 values of 0.12 {mu}M, 0.5 {mu}M, 1.9 {mu}M, and 1.0 {mu}M for BP-1, BP-3, 4-MBC and OMC, respectively. Estradiol (E2) had a C50 value of 4.8 pM. Experiments with equipotent mixtures all supported our null hypothesis that mixtures of UV filters act additively to activate the estrogen receptor (ER). In view of our results and observed plasma levels it cannot be excluded that daily exposure to sunscreen formulations may have estrogenic effects in humans.

Heneweer, Marjoke [Institute for Risk Assessment Sciences (IRAS), Utrecht University, PO Box 80176, 3508 TD Utrecht (Netherlands)]. E-mail: M.Heneweer@iras.uu.nl; Muusse, Martine [Institute for Risk Assessment Sciences (IRAS), Utrecht University, PO Box 80176, 3508 TD Utrecht (Netherlands); Berg, Martin van den [Institute for Risk Assessment Sciences (IRAS), Utrecht University, PO Box 80176, 3508 TD Utrecht (Netherlands); Sanderson, J. Thomas [Institute for Risk Assessment Sciences (IRAS), Utrecht University, PO Box 80176, 3508 TD Utrecht (Netherlands)

2005-10-15T23:59:59.000Z

359

Bonded polyimide fuel cell package  

DOE Patents [OSTI]

Described herein are processes for fabricating microfluidic fuel cell systems with embedded components in which micron-scale features are formed by bonding layers of DuPont Kapton.TM. polyimide laminate. A microfluidic fuel cell system fabricated using this process is also described.

Morse, Jeffrey D.; Jankowski, Alan; Graff, Robert T.; Bettencourt, Kerry

2010-06-08T23:59:59.000Z

360

Careers In Fuel Cell Technologies  

E-Print Network [OSTI]

, to combined heat and power (CHP) units used for distributed electricity generation, to passenger vehicles. Today's Technology and Its Growth Potential Today's fuel cell technology offers cost in hydrogen and fuel cells. Activities have reduced the amount of platinum needed by more than a factor

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Photovoltaic cells employing zinc phosphide  

DOE Patents [OSTI]

A photovoltaic cell having a zinc phosphide absorber. The zinc phosphide can be a single or multiple crystal slice or a thin polycrystalline film. The cell can be a Schottky barrier, heterojunction or homojunction device. Methods for synthesizing and crystallizing zinc phosphide are disclosed as well as a method for forming thin films.

Barnett, Allen M. (Newark, DE); Catalano, Anthony W. (Wilmington, DE); Dalal, Vikram L. (Newark, DE); Masi, James V. (Wilbraham, MA); Meakin, John D. (Newark, DE); Hall, Robert B. (Newark, DE)

1984-01-01T23:59:59.000Z

362

Plastic Schottky barrier solar cells  

DOE Patents [OSTI]

A photovoltaic cell structure is fabricated from an active medium including an undoped, intrinsically p-type organic semiconductor comprising polyacetylene. When a film of such material is in rectifying contact with a magnesium electrode, a Schottky-barrier junction is obtained within the body of the cell structure. Also, a gold overlayer passivates the magnesium layer on the undoped polyacetylene film.

Waldrop, James R. (Thousand Oaks, CA); Cohen, Marshall J. (Thousand Oaks, CA)

1984-01-24T23:59:59.000Z

363

Hydrogen & Fuel Cells -Program Overview -  

E-Print Network [OSTI]

, Panasonic, Delphi Technologies Clean Energy Patent Growth Index Source: Clean Energy Patent Growth Index #12 and Peer Evaluation Meeting May 14, 2012 #12;Petroleum 37% Natural Gas 25% Coal 21% Nuclear Energy 9, 2010 Fuel Cells can apply to diverse sectors #12;3 Fuel Cells ­ An Emerging Global Industry Clean

364

National Fuel Cell Research Center  

E-Print Network [OSTI]

National Fuel Cell Research Center www.nfcrc.uci.edu SOFC AND PEMFC COMPARISON Efficiency ­ Higher operating voltages and temperatures and reduced fuel processing requirements give SOFCs an efficiency FOR OPTIMIZATION · Fuel Cell · Compressor · Combustor · Turbine · Storage Tank · Heat Exchanger·Battery · Motor

Mease, Kenneth D.

365

Energy 101: Fuel Cell Technology  

SciTech Connect (OSTI)

Learn how fuel cell technology generates clean electricity from hydrogen to power our buildings and transportation-while emitting nothing but water. This video illustrates the fundamentals of fuel cell technology and its potential to supply our homes, offices, industries, and vehicles with sustainable, reliable energy.

None

2014-03-11T23:59:59.000Z

366

Energy 101: Fuel Cell Technology  

ScienceCinema (OSTI)

Learn how fuel cell technology generates clean electricity from hydrogen to power our buildings and transportation-while emitting nothing but water. This video illustrates the fundamentals of fuel cell technology and its potential to supply our homes, offices, industries, and vehicles with sustainable, reliable energy.

None

2014-06-06T23:59:59.000Z

367

VERTEX CHAMBERS TARGET CELL CALORIMETER  

E-Print Network [OSTI]

DRIFT VC 1/2 FC 1/2 VERTEX CHAMBERS TARGET CELL DVC MC 1­3 HODOSCOPE H0 MONITOR BC 1/2 BC 3/4 TRD at Threashold Lambda Physics (u ­L spin transfer) Motivation: ­ W L Target cell e beam L p p e ­ Elastic: Peltier elements ( T ~ ­20C ) ­ Custom built electronics + HELIX chips low autgassing (

368

Graphite-based photovoltaic cells  

DOE Patents [OSTI]

The present invention uses lithographically patterned graphite stacks as the basic building elements of an efficient and economical photovoltaic cell. The basic design of the graphite-based photovoltaic cells includes a plurality of spatially separated graphite stacks, each comprising a plurality of vertically stacked, semiconducting graphene sheets (carbon nanoribbons) bridging electrically conductive contacts.

Lagally, Max (Madison, WI); Liu, Feng (Salt Lake City, UT)

2010-12-28T23:59:59.000Z

369

Bronx Zoo Fuel Cell Project  

SciTech Connect (OSTI)

A 200 kW Fuel Cell has been installed in the Lion House, Bronx Zoo, NY. The Fuel Cell is a 200 kW phosphoric acid type manufactured by United Technologies Corporation (UTC) and will provide thermal energy at 725,000 Btu/hr.

Hoang Pham

2007-09-30T23:59:59.000Z

370

Electrode for a lithium cell  

DOE Patents [OSTI]

This invention relates to a positive electrode for an electrochemical cell or battery, and to an electrochemical cell or battery; the invention relates more specifically to a positive electrode for a non-aqueous lithium cell or battery when the electrode is used therein. The positive electrode includes a composite metal oxide containing AgV.sub.3O.sub.8 as one component and one or more other components consisting of LiV.sub.3O.sub.8, Ag.sub.2V.sub.4O.sub.11, MnO.sub.2, CF.sub.x, AgF or Ag.sub.2O to increase the energy density of the cell, optionally in the presence of silver powder and/or silver foil to assist in current collection at the electrode and to improve the power capability of the cell or battery.

Thackeray, Michael M. (Naperville, IL); Vaughey, John T. (Elmhurst, IL); Dees, Dennis W. (Downers Grove, IL)

2008-10-14T23:59:59.000Z

371

Rechargeable lithium-ion cell  

DOE Patents [OSTI]

The invention relates to a rechargeable lithium-ion cell, a method for its manufacture, and its application. The cell is distinguished by the fact that it has a metallic housing (21) which is electrically insulated internally by two half shells (15), which cover electrode plates (8) and main output tabs (7) and are composed of a non-conductive material, where the metallic housing is electrically insulated externally by means of an insulation coating. The cell also has a bursting membrane (4) which, in its normal position, is located above the electrolyte level of the cell (1). In addition, the cell has a twisting protection (6) which extends over the entire surface of the cover (2) and provides centering and assembly functions for the electrode package, which comprises the electrode plates (8).

Bechtold, Dieter (Bad Vilbel, DE); Bartke, Dietrich (Kelkheim, DE); Kramer, Peter (Konigstein, DE); Kretzschmar, Reiner (Kelkheim, DE); Vollbert, Jurgen (Hattersheim, DE)

1999-01-01T23:59:59.000Z

372

Ambient pressure fuel cell system  

DOE Patents [OSTI]

An ambient pressure fuel cell system is provided with a fuel cell stack formed from a plurality of fuel cells having membrane/electrode assemblies (MEAs) that are hydrated with liquid water and bipolar plates with anode and cathode sides for distributing hydrogen fuel gas and water to a first side of each one of the MEAs and air with reactant oxygen gas to a second side of each one of the MEAs. A pump supplies liquid water to the fuel cells. A recirculating system may be used to return unused hydrogen fuel gas to the stack. A near-ambient pressure blower blows air through the fuel cell stack in excess of reaction stoichiometric amounts to react with the hydrogen fuel gas.

Wilson, Mahlon S. (Los Alamos, NM)

2000-01-01T23:59:59.000Z

373

Federico Zenith Control of fuel cells  

E-Print Network [OSTI]

Federico Zenith Control of fuel cells Doctoral thesis for the degree of philosophiæ doctor with control of fuel cells, focusing on high-temperature proton- exchange-membrane fuel cells. Fuel cells-wide electric grids. Whereas studies about the design of fuel cell systems and the electrochemical properties

Skogestad, Sigurd

374

Federico Zenith Control of fuel cells  

E-Print Network [OSTI]

Federico Zenith Control of fuel cells Doctoral thesis for the degree of philosophiæ doctor with control of fuel cells, focusing on high-temperature proton-exchange-membrane fuel cells. Fuel cells-wide electric grids. Whereas studies about the design of fuel cell systems and the electrochemical properties

Skogestad, Sigurd

375

Microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography  

SciTech Connect (OSTI)

High-pressure freezing is the preferred method to prepare thick biological specimens for ultrastructural studies. However, the advantages obtained by this method often prove unattainable for samples that are difficult to handle during the freezing and substitution protocols. Delicate and sparse samples are difficult to manipulate and maintain intact throughout the sequence of freezing, infiltration, embedding, and final orientation for sectioning and subsequent TEM imaging. An established approach to surmount these difficulties is the use of cellulose microdialysis tubing to transport the sample. With an inner diameter of 200 micrometers, the tubing protects small and fragile samples within the thickness constraints of high-pressure freezing, and the tube ends can be sealed to avoid loss of sample. Importantly, the transparency of the tubing allows optical study of the specimen at different steps in the process. Here, we describe the use of a micromanipulator and microinjection apparatus to handle and position delicate specimens within the tubing. We report two biologically significant examples that benefit from this approach, 3D cultures of mammary epithelial cells and cochlear outer hair cells. We illustrate the potential for correlative light and electron microscopy as well as electron tomography.

Auer, Manfred; Triffo, W.J.; Palsdottir, H.; McDonald, K.L.; Inman, J.L.; Bissell, M.J.; Raphael, R.M.; Auer, M.; Lee, J.K.

2008-02-13T23:59:59.000Z

376

Red Blood cell Alloimmunization in Sickle Cell Disease: Pathophysiology, Risk Factors, and Transfusion Management  

E-Print Network [OSTI]

1 Red Blood cell Alloimmunization in Sickle Cell Disease: Pathophysiology, Risk Factors; email: france.noizat-pirenne@efs.sante.fr Keywords: sickle cell disease, alloimmunization, DHTR with sickle cell disease (SCD). Transfusions can lead to erythrocyte alloimmunization, however, with serious

Boyer, Edmond

377

Collagen-Hyaluronic Acid Scaffolds for Adipose Tissue Engineering  

E-Print Network [OSTI]

-drying, also known as lyophilization, is commonly employed to produce water-soluble polymer scaffolds such as collagen [12, 41-43]. In this technique, a suspension of the water-soluble polymer is frozen, thereby forming an interpenetrating network of ice... . Keywords: Collagen; crosslinking; freeze-drying; hyaluronic acid; scaffolds, adipose tissue engineering 1. Introduction The mammary gland comprises a complex branched epithelial network invested within an adipocyte-rich stroma termed a fat...

Davidenko, Natalia; Campbell, J. J.; Thian, E. S.; Watson, C. J,; Cameron, Ruth Elizabeth

2010-01-01T23:59:59.000Z

378

Pearling in cells: A clue to understanding cell shape  

E-Print Network [OSTI]

Gradual disruption of the actin cytoskeleton induces a series of structural shape changes in cells leading to a transformation of cylindrical cell extensions into a periodic chain of "pearls". Quantitative measurements of the pearling instability give a square-root behavior for the wavelength as a function of drug concentration. We present a theory that explains these observations in terms of the interplay between rigidity of the submembranous actin shell and tension that is induced by boundary conditions set by adhesion points. The theory allows estimation of the rigidity and thickness of this supporting shell. The same theoretical considerations explain the shape of nonadherent edges in the general case of untreated cells.

Roy Bar-Ziv; Tsvi Tlusty; Elisha Moses; Samuel A. Safran; Alexander Bershadsky

2010-08-05T23:59:59.000Z

379

Fuel Cell Research  

SciTech Connect (OSTI)

Executive Summary In conjunction with the Brown Energy Initiative, research Projects selected for the fuel cell research grant were selected on the following criteria: ? They should be fundamental research that has the potential to significantly impact the nation’s energy infrastructure. ? They should be scientifically exciting and sound. ? They should synthesize new materials, lead to greater insights, explore new phenomena, or design new devices or processes that are of relevance to solving the energy problems. ? They involve top-caliper senior scientists with a record of accomplishment, or junior faculty with outstanding promise of achievement. ? They should promise to yield at least preliminary results within the given funding period, which would warrant further research development. ? They should fit into the overall mission of the Brown Energy Initiative, and the investigators should contribute as partners to an intellectually stimulating environment focused on energy science. Based on these criteria, fourteen faculty across three disciplines (Chemistry, Physics and Engineering) and the Charles Stark Draper Laboratory were selected to participate in this effort.1 In total, there were 30 people supported, at some level, on these projects. This report highlights the findings and research outcomes of the participating researchers.

Weber, Peter M. [Brown University] [Brown University

2014-03-30T23:59:59.000Z

380

Fuel Cells for Transportation | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

DOE R&D Activities Fuel Cells for Transportation Fuel Cells for Transportation Photo of Ford Focus fuel cell car in front of windmills The transportation sector is the single...

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Control of Transcription by Cell Size  

E-Print Network [OSTI]

Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. ...

Wu, Chia-Yung

382

The challenges of organic polymer solar cells  

E-Print Network [OSTI]

The technical and commercial prospects of polymer solar cells were evaluated. Polymer solar cells are an attractive approach to fabricate and deploy roll-to-roll processed solar cells that are reasonably efficient (total ...

Saif Addin, Burhan K. (Burhan Khalid)

2011-01-01T23:59:59.000Z

383

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

Sediment microbial fuel cells demonstrating marine (left)5 FutureWork 5.1 Microfluidic Microbial Fuel Cell Continuedthe micro- patterned microbial fuel cell. Note that V oc,max

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

384

Planning a Commercial Fuel Cell Installation  

E-Print Network [OSTI]

the alkaline and molten carbonate cells as they may have special ap~lications since their operating characteristics are noticeably different from the phosphoric acid cells. Next, the Los Alamos study concludes that the phosphoric acid fuel cell matches...

Bowden, J. R.; May, G. W.

385

Breaking the Fuel Cell Cost Barrier  

Broader source: Energy.gov (indexed) [DOE]

the Fuel Cell Cost Barrier AMFC Workshop May 8 th , 2011, Arlington, VA Shimshon Gottesfeld, CTO The Fuel Cell Cost Challenge 2 CellEra's goal - achieve price parity with...

386

Furman University Cell Phone Allowance Request Form  

E-Print Network [OSTI]

Furman University Cell Phone Allowance Request Form Date Payment: $___________ All cell phone allowance payments are departmental responsibility and considered other compensation charged to object code ________. The cell phone allowance will start at the next

387

Fabrication and Characterization of Organic Solar Cells  

E-Print Network [OSTI]

Würfel P.  Physics of solar cells : from principles to new generation  photovoltaics:  solar  cells  for  2020  and Spitzer  MB.   INDIUM?PHOSPHIDE  SOLAR?CELLS  MADE  BY  ION?

Yengel, Emre

2010-01-01T23:59:59.000Z

388

Commercialization of Novel Organic Solar Cells  

E-Print Network [OSTI]

Commercialization of Novel Organic Solar Cells Master of Engineering Final Report Shanel C. Miller................................................................................................................... 12 2.1 How do Solar Cells Work?.................................................................................................. 12 2.2 Types of Solar Cells that Exist Today

Kassegne, Samuel Kinde

389

Nontoxic quantum dot research improves solar cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Nontoxic quantum dot research improves solar cells Nontoxic quantum dot research improves solar cells Solar cells made with low-cost, nontoxic copper-based quantum dots can achieve...

390

Photovoltaic Cell Material Basics | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

cell depends on two factors: the material of the cell and the wavelength or energy of the light being absorbed. Solar cell material has an abrupt edge in its absorption coefficient...

391

MICROFLUIDIC CONTROL OF STEM CELL DIFFUSIBLE SIGNALING  

E-Print Network [OSTI]

MICROFLUIDIC CONTROL OF STEM CELL DIFFUSIBLE SIGNALING Katarina Blagovi, Lily Y. Kim, Alison M cell differentiation. KEYWORDS: Embryonic stem cells, microfluidic perfusion, diffusible signaling; they secrete molecules to which they respond. Microfluidics offers a potential solution to this challenge

Voldman, Joel

392

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

tion, to the typical PEM fuel cell kinetics, the system alsostudied. As with other PEM fuel cells, it is generally ad-exchange membrane (PEM) fuel cell performance, utilizing

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

393

Air Breathing Direct Methanol Fuel Cell  

DOE Patents [OSTI]

A method for activating a membrane electrode assembly for a direct methanol fuel cell is disclosed. The method comprises operating the fuel cell with humidified hydrogen as the fuel followed by running the fuel cell with methanol as the fuel.

Ren; Xiaoming (Los Alamos, NM)

2003-07-22T23:59:59.000Z

394

Fabricate PHEV Cells for Testing & Diagnostics  

Broader source: Energy.gov (indexed) [DOE]

Identify vendors to make electrodes and pouch18650 cells for ABR Jan., 2009 Finalize order of pouch18650 cells with vendors April, 2009 Distribute vendor cells to ABR for...

395

Fuel Cells Get New BFF | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cells Get New BFF Fuel Cells Get New BFF Artificial diamonds may lead to affordable, efficient fuel cells Oxygen (red spheres) migrates from one vacancy to another inside the...

396

Generation of ovine induced pluripotent stem cells   

E-Print Network [OSTI]

Embryonic stem cells (ESCs) are pluripotent cells derived from the early embryo and are able to differentiate into cells belonging to the three germ layers. They are a valuable tool in research and for clinical use, but ...

Sartori, Chiara

2012-06-30T23:59:59.000Z

397

Microfluidic Microbial Fuel Cells for Microstructure Interrogations  

E-Print Network [OSTI]

5 FutureWork 5.1 Microfluidic Microbial Fuel Cell ContinuedModel of hydrogen fuel cell kinetic losses includingSediment microbial fuel cells demonstrating marine (left)

Parra, Erika Andrea

2010-01-01T23:59:59.000Z

398

How Fuel Cells Work | Department of Energy  

Energy Savers [EERE]

Fuel Cells Work How Energy Works 30 likes How Fuel Cells Work Fuel cells produce electrical power without any combustion and operate on fuels like hydrogen, natural gas and...

399

Solar-Hydrogen Fuel-Cell Vehicles  

E-Print Network [OSTI]

M. A. (1992). Hydrogen Fuel-Cell Vehicles. Re- koebensteinthan both. Solar-hydrogen and fuel-cell vehicles wouldberegulation. Solar-Hydrogen Fuel-Cell Vehicles MarkA. DeLuchi

DeLuchi, Mark A.; Ogden, Joan M.

1993-01-01T23:59:59.000Z

400

Climate Change Fuel Cell Program  

SciTech Connect (OSTI)

Verizon is presently operating the largest Distributed Generation Fuel Cell project in the USA. Situated in Long Island, NY, the power plant is composed of seven (7) fuel cells operating in parallel with the Utility grid from the Long Island Power Authority (LIPA). Each fuel cell has an output of 200 kW, for a total of 1.4 mW generated from the on-site plant. The remaining power to meet the facility demand is purchased from LIPA. The fuel cell plant is utilized as a co-generation system. A by-product of the fuel cell electric generation process is high temperature water. The heat content of this water is recovered from the fuel cells and used to drive two absorption chillers in the summer and a steam generator in the winter. Cost savings from the operations of the fuel cells are forecasted to be in excess of $250,000 per year. Annual NOx emissions reductions are equivalent to removing 1020 motor vehicles from roadways. Further, approximately 5.45 million metric tons (5 millions tons) of CO2 per year will not be generated as a result of this clean power generation. The project was partially financed with grants from the New York State Energy R&D Authority (NYSERDA) and from Federal Government Departments of Defense and Energy.

Paul Belard

2006-09-21T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Climate Change Fuel Cell Program  

SciTech Connect (OSTI)

A 200 kW, natural gas fired fuel cell was installed at the Richard Stockton College of New Jersey. The purpose of this project was to demonstrate the financial and operational suitability of retrofit fuel cell technology at a medium sized college. Target audience was design professionals and the wider community, with emphasis on use in higher education. ''Waste'' heat from the fuel cell was utilized to supplement boiler operations and provide domestic hot water. Instrumentation was installed in order to measure the effectiveness of heat utilization. It was determined that 26% of the available heat was captured during the first year of operation. The economics of the fuel cell is highly dependent on the prices of electricity and natural gas. Considering only fuel consumed and energy produced (adjusted for boiler efficiency), the fuel cell saved $54,000 in its first year of operation. However, taking into account the price of maintenance and the cost of financing over the short five-year life span, the fuel cell operated at a loss, despite generous subsidies. As an educational tool and market stimulus, the fuel cell attracted considerable attention, both from design professionals and the general public.

Alice M. Gitchell

2006-09-15T23:59:59.000Z

402

DOE Fuel Cell Technologies Program Record, Record # 11003, Fuel...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

DOE Fuel Cell Technologies Program Record, Record 11003, Fuel Cell Stack Durability DOE Fuel Cell Technologies Program Record, Record 11003, Fuel Cell Stack Durability Dated...

403

DOE Cell Component Accelerated Stress Test Protocols for PEM...  

Broader source: Energy.gov (indexed) [DOE]

Cell Component Accelerated Stress Test Protocols for PEM Fuel Cells DOE Cell Component Accelerated Stress Test Protocols for PEM Fuel Cells This document describes test protocols...

404

Overview of Fuel Cell Electric Bus Development | Department of...  

Broader source: Energy.gov (indexed) [DOE]

Fuel Cell Electric Bus Development Overview of Fuel Cell Electric Bus Development Presentation slides from the Fuel Cell Technologies Office webinar ""Fuel Cell Buses"" held...

405

Overview of Hydrogen and Fuel Cell Activities: 2011 IPHE Stationary...  

Broader source: Energy.gov (indexed) [DOE]

Overview of Hydrogen and Fuel Cell Activities: 2011 IPHE Stationary Fuel Cell Workshop Overview of Hydrogen and Fuel Cell Activities: 2011 IPHE Stationary Fuel Cell Workshop...

406

Cell Component Accelerated Stress Test Protocols for PEM Fuel...  

Broader source: Energy.gov (indexed) [DOE]

Cell Component Accelerated Stress Test Protocols for PEM Fuel Cells Cell Component Accelerated Stress Test Protocols for PEM Fuel Cells Accelerated Stress Test Protocols for PEM...

407

Overview of Hydrogen and Fuel Cell Activities: 2011 IPHE Stationary...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Cell Activities: 2011 IPHE Stationary Fuel Cell Workshop Overview of Hydrogen and Fuel Cell Activities: 2011 IPHE Stationary Fuel Cell Workshop Presentation by Rick Farmer at the...

408

1986 fuel cell seminar: Program and abstracts  

SciTech Connect (OSTI)

Ninety nine brief papers are arranged under the following session headings: gas industry's 40 kw program, solid oxide fuel cell technology, phosphoric acid fuel cell technology, molten carbonate fuel cell technology, phosphoric acid fuel cell systems, power plants technology, fuel cell power plant designs, unconventional fuels, fuel cell application and economic assessments, and plans for commerical development. The papers are processed separately for the data base. (DLC)

none,

1986-10-01T23:59:59.000Z

409

Solid oxide fuel cell generator  

DOE Patents [OSTI]

A solid oxide fuel cell generator has a pair of spaced apart tubesheets in a housing. At least two intermediate barrier walls are between the tubesheets and define a generator chamber between two intermediate buffer chambers. An array of fuel cells have tubes with open ends engaging the tubesheets. Tubular, axially elongated electrochemical cells are supported on the tubes in the generator chamber. Fuel gas and oxidant gas are preheated in the intermediate chambers by the gases flowing on the other side of the tubes. Gas leakage around the tubes through the tubesheets is permitted. The buffer chambers reentrain the leaked fuel gas for reintroduction to the generator chamber.

Draper, R.; George, R.A.; Shockling, L.A.

1993-04-06T23:59:59.000Z

410

Mixed ternary heterojunction solar cell  

DOE Patents [OSTI]

A thin film heterojunction solar cell and a method of making it has a p-type layer of mixed ternary I-III-VI.sub.2 semiconductor material in contact with an n-type layer of mixed binary II-VI semiconductor material. The p-type semiconductor material includes a low resistivity copper-rich region adjacent the back metal contact of the cell and a composition gradient providing a minority carrier mirror that improves the photovoltaic performance of the cell. The p-type semiconductor material preferably is CuInGaSe.sub.2 or CuIn(SSe).sub.2.

Chen, Wen S. (Seattle, WA); Stewart, John M. (Seattle, WA)

1992-08-25T23:59:59.000Z

411

Organic fuel cells and fuel cell conducting sheets  

DOE Patents [OSTI]

A passive direct organic fuel cell includes an organic fuel solution and is operative to produce at least 15 mW/cm.sup.2 when operating at room temperature. In additional aspects of the invention, fuel cells can include a gas remover configured to promote circulation of an organic fuel solution when gas passes through the solution, a modified carbon cloth, one or more sealants, and a replaceable fuel cartridge.

Masel, Richard I. (Champaign, IL); Ha, Su (Champaign, IL); Adams, Brian (Savoy, IL)

2007-10-16T23:59:59.000Z

412

Hydrogen, Fuel Cells and Infrastructure Technologies Program...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Hydrogen, Fuel Cells and Infrastructure Technologies Program FY2003 Merit Review and Peer Evaluation Report Hydrogen, Fuel Cells and Infrastructure Technologies Program FY2003...

413

Webinar: National Fuel Cell Technology Evaluation Center  

Broader source: Energy.gov [DOE]

Video recording and text version of the Fuel Cell Technologies Office webinar titled "National Fuel Cell Technology Evaluation Center (NFCTEC)," originally presented on March 11, 2014.

414

Fuel Cell Technology Challenges | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Technology Challenges Fuel Cell Technology Challenges Cost and durability are the major challenges to fuel cell commercialization. However, hurdles vary according to the...

415

Ames Lab 101: Improving Solar Cell Efficiency  

SciTech Connect (OSTI)

Rana Biswas, a scientist with the Ames Laboratory, discusses his team's research in creating more efficient solar cells and working with Iowa Thin Film to produce these cells.

Biswas, Rana

2011-01-01T23:59:59.000Z

416

Ames Lab 101: Improving Solar Cell Efficiency  

ScienceCinema (OSTI)

Rana Biswas, a scientist with the Ames Laboratory, discusses his team's research in creating more efficient solar cells and working with Iowa Thin Film to produce these cells.

Biswas, Rana

2012-08-29T23:59:59.000Z

417

Screen Electrode Materials & Cell Chemistries and Streamlining...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

& Cell Chemistries and Streamlining Optimization of Electrode Screen Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrode 2010 DOE Vehicle Technologies...

418

ELECTROSPUN POLYMER-FIBER SOLAR CELL.  

E-Print Network [OSTI]

??A study of fabricating the first electrospun polymer-fiber solar cell with MEHPPV is presented. Motivation for the work and a brief history of solar cell… (more)

Nagata, Shinobu

2011-01-01T23:59:59.000Z

419

Webinar: Advanced Electrocatalysts for PEM Fuel Cells  

Broader source: Energy.gov [DOE]

Video recording of the Fuel Cell Technologies Office webinar, Advanced Electrocatalysts for PEM Fuel Cells, originally presented on February 12, 2013.

420

Cell Phone Carriers, TV-Commercials & Branding.  

E-Print Network [OSTI]

?? Problem: As almost everyone has a cell phone today, keeping your customers is very important. An important group for cell phone carriers is young… (more)

Sköld, Robin

2009-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

Canadian Fuel Cell Commercialization Roadmap Update: Progress...  

Open Energy Info (EERE)

Commercialization Roadmap Update: Progress of Canada's Hydrogen and Fuel Cell Industry Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Canadian Fuel Cell...

422

Fuel Cell Animation- Chemical Process (Text Version)  

Broader source: Energy.gov [DOE]

This text version of the fuel cell animation demonstrates how a fuel cell uses hydrogen to produce electricity, with only water and heat as byproducts.

423

Biomimetic Dye Molecules for Solar Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

such as those used in solar cells. This requires close monitoring to obtain reproducible solar cells. The polarization dependence of the spectra reveals the orientation of the...

424

Characterization of Fuel-Cell Diffusion Media  

E-Print Network [OSTI]

47 Figure 4.2 CV of PEM fuel-cell CL that shows hydrogencurrent. Figure 4.2. CV of PEM fuel-cell catalyst layer that

Gunterman, Haluna Penelope Frances

2011-01-01T23:59:59.000Z

425

Market Transformation: Fuel Cell Early Adoption (Presentation...  

Office of Environmental Management (EM)

Fuel Cell Technologies Office Hydrogen Production Hydrogen Delivery Hydrogen Storage Fuel Cells Technology Validation Manufacturing Safety, Codes, and Standards Education Market...

426

Hydrogen and Fuel Cells | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Transportation Hydrogen and Fuel Cells Hydrogen and Fuel Cells EERE leads U.S. researchers and other partners in making transportation cleaner and more efficient through...

427

Fuel Cell & Hydrogen Technologies | Clean Energy | ORNL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fuel Cell Technologies SHARE Fuel Cell and Hydrogen Technologies Oak Ridge National Laboratory pursues activities that address the barriers facing the development and deployment of...

428

Hydrogen, Fuel Cells and Infrastructure Technologies Program...  

Broader source: Energy.gov (indexed) [DOE]

Hydrogen, Fuel Cells and Infrastructure Technologies Program: 2002 Annual Progress Report Hydrogen, Fuel Cells and Infrastructure Technologies Program: 2002 Annual Progress Report...

429

Human papillomavirus 16 E5 induces bi-nucleated cell formation by cell-cell fusion  

SciTech Connect (OSTI)

Human papillomaviruses (HPV) 16 is a DNA virus encoding three oncogenes - E5, E6, and E7. The E6 and E7 proteins have well-established roles as inhibitors of tumor suppression, but the contribution of E5 to malignant transformation is controversial. Using spontaneously immortalized human keratinocytes (HaCaT cells), we demonstrate that expression of HPV16 E5 is necessary and sufficient for the formation of bi-nucleated cells, a common characteristic of precancerous cervical lesions. Expression of E5 from non-carcinogenic HPV6b does not produce bi-nucleate cells. Video microscopy and biochemical analyses reveal that bi-nucleates arise through cell-cell fusion. Although most E5-induced bi-nucleates fail to propagate, co-expression of HPV16 E6/E7 enhances the proliferation of these cells. Expression of HPV16 E6/E7 also increases bi-nucleated cell colony formation. These findings identify a new role for HPV16 E5 and support a model in which complementary roles of the HPV16 oncogenes lead to the induction of carcinogenesis.

Hu Lulin; Plafker, Kendra [Department of Cell Biology, University of Oklahoma (United States); Vorozhko, Valeriya [Department of Cell Biology, University of Oklahoma (United States); Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation (United States); Zuna, Rosemary E. [Department of Pathology, University of Oklahoma HSC (United States); Hanigan, Marie H. [Department of Cell Biology, University of Oklahoma (United States); Gorbsky, Gary J. [Department of Cell Biology, University of Oklahoma (United States); Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation (United States); Plafker, Scott M. [Department of Cell Biology, University of Oklahoma (United States); Angeletti, Peter C. [Nebraska Center for Virology (United States); Ceresa, Brian P. [Department of Cell Biology, University of Oklahoma (United States)], E-mail: brian-ceresa@oushc.edu

2009-02-05T23:59:59.000Z

430

NETL: Solid Oxide Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and water concerns associated with fossil fuel based electric power generation. The NETL Fuel Cell Program maintains a portfolio of RD&D projects that address the technical issues...

431

Glycosaminoglycan regulation of cell function  

E-Print Network [OSTI]

Glycosaminoglycans (GAGs) are complex polysaccharides that exist both on the cell surface and free within the extracellular matrix. The intrinsic sequence variety stemming from the large number of building blocks that ...

Berry, David (David A.)

2005-01-01T23:59:59.000Z

432

Fuel Cell Kickoff Meeting Agenda  

Broader source: Energy.gov (indexed) [DOE]

Hamrock, 3M 9:40 New Polyelectrolyte Materials for High Temperature Fuel Cells J. Kerr, LBNL 10:00 The Design of Novel Materials Consisting of a Semi- Interpenetrating Network of...

433

Dye-Sensitized Solar Cells  

Broader source: Energy.gov [DOE]

DOE supports research and development projects aimed at increasing the efficiency and lifetime of dye-sensitized solar cells (DSSCs). Below are a list of current projects, summary of the benefits,...

434

Interfacing nanostructures to biological cells  

DOE Patents [OSTI]

Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated .alpha.-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the .alpha.-N-acetylgalactosamine groups on the nanostructure.

Chen, Xing; Bertozzi, Carolyn R.; Zettl, Alexander K.

2012-09-04T23:59:59.000Z

435

CLIMATE CHANGE FUEL CELL PROGRAM  

SciTech Connect (OSTI)

ChevronTexaco has successfully operated a 200 kW PC25C phosphoric acid fuel cell power plant at the corporate data center in San Ramon, California for the past two years and seven months following installation in December 2001. This site was chosen based on the ability to utilize the combined heat (hot water) and power generation capability of this modular fuel cell power plant in an office park setting . In addition, this project also represents one of the first commercial applications of a stationary fuel cell for a mission critical data center to assess power reliability benefits. This fuel cell power plant system has demonstrated outstanding reliability and performance relative to other comparably sized cogeneration systems.

Mike Walneuski

2004-09-16T23:59:59.000Z

436

Hydrogen,Fuel Cells & Infrastructure  

E-Print Network [OSTI]

;The President's FY04 Budget Request for FreedomCAR and Hydrogen Fuel Initiatives 4.0Office of Nuclear commercialization decision by 2015. Fuel Cell Vehicles in the Showroom and Hydrogen at Fueling Stations by 2020 #12

437

IAP Antagonists Promote Cell Death  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the addition of ubiquitin to the target protein substrate, thus marking it for degradation by the cell's proteasome. An x-ray crystal structure of monomeric cIAP1-B3R...

438

Navy fuel cell demonstration project.  

SciTech Connect (OSTI)

This is the final report on a field evaluation by the Department of the Navy of twenty 5-kW PEM fuel cells carried out during 2004 and 2005 at five Navy sites located in New York, California, and Hawaii. The key objective of the effort was to obtain an engineering assessment of their military applications. Particular issues of interest were fuel cell cost, performance, reliability, and the readiness of commercial fuel cells for use as a standalone (grid-independent) power option. Two corollary objectives of the demonstration were to promote technological advances and to improve fuel performance and reliability. From a cost perspective, the capital cost of PEM fuel cells at this stage of their development is high compared to other power generation technologies. Sandia National Laboratories technical recommendation to the Navy is to remain involved in evaluating successive generations of this technology, particularly in locations with greater environmental extremes, and it encourages their increased use by the Navy.

Black, Billy D.; Akhil, Abbas Ali

2008-08-01T23:59:59.000Z

439

CELL BIOLOGY Home-grownfatcontrol  

E-Print Network [OSTI]

, Massachusetts, and his colleagues studied knockout mice lacking two proteins that normally chaperone lipids out-fat diet. The team found increased synthesis of palmitoleate and other unsaturated fatty acids in fat cells

Cai, Long

440

Semi-finished modular cells  

E-Print Network [OSTI]

This thesis subject is a pre-fabricated element (cell): a system that employs natural, light, and economic materials to produce a near-finished portion of a building. The intent is to introduce sustainable design into ...

Bachelder, Laura Govoni, 1971-

2002-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Fuel cell electric power production  

DOE Patents [OSTI]

A process for generating electricity from a fuel cell includes generating a hydrogen-rich gas as the fuel for the fuel cell by treating a hydrocarbon feed, which may be a normally liquid feed, in an autothermal reformer utilizing a first monolithic catalyst zone having palladium and platinum catalytic components therein and a second, platinum group metal steam reforming catalyst. Air is used as the oxidant in the hydrocarbon reforming zone and a low oxygen to carbon ratio is maintained to control the amount of dilution of the hydrogen-rich gas with nitrogen of the air without sustaining an insupportable amount of carbon deposition on the catalyst. Anode vent gas may be utilized as the fuel to preheat the inlet stream to the reformer. The fuel cell and the reformer are preferably operated at elevated pressures, up to about a pressure of 150 psia for the fuel cell.

Hwang, Herng-Shinn (Livingston, NJ); Heck, Ronald M. (Frenchtown, NJ); Yarrington, Robert M. (Westfield, NJ)

1985-01-01T23:59:59.000Z

442

PEM/SPE fuel cell  

DOE Patents [OSTI]

A PEM/SPE fuel cell is described including a membrane-electrode assembly (MEA) having a plurality of oriented filament embedded the face thereof for supporting the MEA and conducting current therefrom to contiguous electrode plates. 4 figs.

Grot, S.A.

1998-01-13T23:59:59.000Z

443

PEM/SPE fuel cell  

DOE Patents [OSTI]

A PEM/SPE fuel cell including a membrane-electrode assembly (MEA) having a plurality of oriented filament embedded the face thereof for supporting the MEA and conducting current therefrom to contiguous electrode plates.

Grot, Stephen Andreas (Henrietta, NY)

1998-01-01T23:59:59.000Z

444

Fuel cell electric power production  

SciTech Connect (OSTI)

A process for generating electricity from a fuel cell includes generating a hydrogen-rich gas as the fuel for the fuel cell by treating a hydrocarbon feed, which may be a normally liquid feed, in an autothermal reformer utilizing a first monolithic catalyst zone having palladium and platinum catalytic components therein and a second, platinum group metal steam reforming catalyst. Air is used as the oxidant in the hydrocarbon reforming zone and a low oxygen to carbon ratio is maintained to control the amount of dilution of the hydrogen-rich gas with nitrogen of the air without sustaining an insupportable amount of carbon deposition on the catalyst. Anode vent gas may be utilized as the fuel to preheat the inlet stream to the reformer. The fuel cell and the reformer are preferably operated at elevated pressures, up to about a pressure of 150 psia for the fuel cell.

Hwang, H.-S.; Heck, R. M.; Yarrington, R. M.

1985-06-11T23:59:59.000Z

445

Imaging Liquids Using Microfluidic Cells  

SciTech Connect (OSTI)

Chemistry occurring in the liquid and liquid surface is important in many applications. Chemical imaging of liquids using vacuum based analytical techniques is challenging due to the difficulty in working with liquids with high volatility. Recent development in microfluidics enabled and increased our capabilities to study liquid in situ using surface sensitive techniques such as electron microscopy and spectroscopy. Due to its small size, low cost, and flexibility in design, liquid cells based on microfluidics have been increasingly used in studying and imaging complex phenomena involving liquids. This paper presents a review of microfluidic cells that were developed to adapt to electron microscopes and various spectrometers for in situ chemical analysis and imaging of liquids. The following topics will be covered including cell designs, fabrication techniques, unique technical features for vacuum compatible cells, and imaging with electron microscopy and spectroscopy. Challenges are summarized and recommendations for future development priority are proposed.

Yu, Xiao-Ying; Liu, Bingwen; Yang, Li

2013-05-10T23:59:59.000Z

446

Additive Manufacturing for Fuel Cells  

Office of Energy Efficiency and Renewable Energy (EERE)

Blake Marshall, AMO's lead for Additive Manufacturing Technologies, will provide an overview of current R&D activities in additive manufacturing and its application to fuel cell prototyping and...

447

Corrosion resistant PEM fuel cell  

DOE Patents [OSTI]

The present invention contemplates a PEM fuel cell having electrical contact elements (including bipolar plates/septums) comprising a titanium nitride coated light weight metal (e.g., Al or Ti) core, having a passivating, protective metal layer intermediate the core and the titanium nitride. The protective layer forms a barrier to further oxidation/corrosion when exposed to the fuel cell's operating environment. Stainless steels rich in CR, Ni, and Mo are particularly effective protective interlayers.

Li, Yang (Troy, MI); Meng, Wen-Jin (Okemos, MI); Swathirajan, Swathy (West Bloomfield, MI); Harris, Stephen Joel (Bloomfield, MI); Doll, Gary Lynn (Orion Township, Oakland County, MI)

2002-01-01T23:59:59.000Z

448

Corrosion resistant PEM fuel cell  

DOE Patents [OSTI]

The present invention contemplates a PEM fuel cell having electrical contact elements (including bipolar plates/septums) comprising a titanium nitride coated light weight metal (e.g., Al or Ti) core, having a passivating, protective metal layer intermediate the core and the titanium nitride. The protective layer forms a barrier to further oxidation/corrosion when exposed to the fuel cell's operating environment. Stainless steels rich in CR, Ni, and Mo are particularly effective protective interlayers.

Li, Yang (Troy, MI); Meng, Wen-Jin (Okemos, MI); Swathirajan, Swathy (West Bloomfield, MI); Harris, Stephen J. (Bloomfield, MI); Doll, Gary L. (Orion Township, Oakland County, MI)

1997-01-01T23:59:59.000Z

449

Air breathing lithium power cells  

DOE Patents [OSTI]

A cell suitable for use in a battery according to one embodiment includes a catalytic oxygen cathode; a stabilized zirconia electrolyte for selective oxygen anion transport; a molten salt electrolyte; and a lithium-based anode. A cell suitable for use in a battery according to another embodiment includes a catalytic oxygen cathode; an electrolyte; a membrane selective to molecular oxygen; and a lithium-based anode.

Farmer, Joseph C.

2014-07-15T23:59:59.000Z

450

PEM fuel cell monitoring system  

DOE Patents [OSTI]

Method and apparatus for monitoring the performance of H.sub.2 --O.sub.2 PEM fuel cells. Outputs from a cell/stack voltage monitor and a cathode exhaust gas H.sub.2 sensor are corrected for stack operating conditions, and then compared to predetermined levels of acceptability. If certain unacceptable conditions coexist, an operator is alerted and/or corrective measures are automatically undertaken.

Meltser, Mark Alexander (Pittsford, NY); Grot, Stephen Andreas (West Henrietta, NY)

1998-01-01T23:59:59.000Z

451

Stationary Fuel Cell Evaluation (Presentation)  

SciTech Connect (OSTI)

This powerpoint presentation discusses its objectives: real world operation data from the field and state-of-the-art lab; collection; analysis for independent technology validation; collaboration with industry and end users operating stationary fuel cell systems and reporting on technology status, progress and technical challenges. The approach and accomplishments are: A quarterly data analysis and publication of first technical stationary fuel cell composite data products (data through June 2012).

Kurtz, J.; Wipke, K.; Sprik, S.; Ramsden, T.; Ainscough, C.

2012-05-01T23:59:59.000Z

452

PEM fuel cell monitoring system  

DOE Patents [OSTI]

Method and apparatus are disclosed for monitoring the performance of H{sub 2}--O{sub 2} PEM fuel cells. Outputs from a cell/stack voltage monitor and a cathode exhaust gas H{sub 2} sensor are corrected for stack operating conditions, and then compared to predetermined levels of acceptability. If certain unacceptable conditions coexist, an operator is alerted and/or corrective measures are automatically undertaken. 2 figs.

Meltser, M.A.; Grot, S.A.

1998-06-09T23:59:59.000Z

453

Module level solutions to solar cell polarization  

DOE Patents [OSTI]

A solar cell module includes interconnected solar cells, a transparent cover over the front sides of the solar cells, and a backsheet on the backsides of the solar cells. The solar cell module includes an electrical insulator between the transparent cover and the front sides of the solar cells. An encapsulant protectively packages the solar cells. To prevent polarization, the insulator has resistance suitable to prevent charge from leaking from the front sides of the solar cells to other portions of the solar cell module by way of the transparent cover. The insulator may be attached (e.g., by coating) directly on an underside of the transparent cover or be a separate layer formed between layers of the encapsulant. The solar cells may be back junction solar cells.

Xavier, Grace (Fremont, CA), Li; Bo (San Jose, CA)

2012-05-29T23:59:59.000Z

454

Fuel cell with internal flow control  

DOE Patents [OSTI]

A fuel cell stack is provided with a plurality of fuel cell cassettes where each fuel cell cassette has a fuel cell with an anode and cathode. The fuel cell stack includes an anode supply chimney for supplying fuel to the anode of each fuel cell cassette, an anode return chimney for removing anode exhaust from the anode of each fuel cell cassette, a cathode supply chimney for supplying oxidant to the cathode of each fuel cell cassette, and a cathode return chimney for removing cathode exhaust from the cathode of each fuel cell cassette. A first fuel cell cassette includes a flow control member disposed between the anode supply chimney and the anode return chimney or between the cathode supply chimney and the cathode return chimney such that the flow control member provides a flow restriction different from at least one other fuel cell cassettes.

Haltiner, Jr., Karl J. (Fairport, NY); Venkiteswaran, Arun (Karnataka, IN)

2012-06-12T23:59:59.000Z

455

Small cell lung cancer and cancer stem cell-like cells   

E-Print Network [OSTI]

Small cell lung cancer (SCLC) is a highly aggressive malignancy with extreme mortality and morbidity. Although initially chemo- and radio-sensitive, almost inevitable recurrence and resistance occurs. SCLC patients often ...

Sarvi, Sana

2014-07-05T23:59:59.000Z

456

Microfluidic Platforms for Studies of Angiogenesis, Cell Migration, and Cell–Cell Interactions  

E-Print Network [OSTI]

Recent advances in microfluidic technologies have opened the door for creating more realistic in vitro cell culture methods that replicate many aspects of the true in vivo microenvironment. These new designs (i) provide ...

Chung, Seok

457

Study of cell-cell communication using 3D living cell microarrays  

E-Print Network [OSTI]

Cellular behavior is not dictated solely from within; it is also guided by a myriad of external cues. If cells are removed from their natural environment, apart from the microenvironment and social context they are accustomed ...

Timp, Winston (Winston G.)

2007-01-01T23:59:59.000Z

458

Solar cell with back side contacts  

DOE Patents [OSTI]

A III-V solar cell is described herein that includes all back side contacts. Additionally, the positive and negative electrical contacts contact compoud semiconductor layers of the solar cell other than the absorbing layer of the solar cell. That is, the positive and negative electrical contacts contact passivating layers of the solar cell.

Nielson, Gregory N; Okandan, Murat; Cruz-Campa, Jose Luis; Resnick, Paul J; Wanlass, Mark Woodbury; Clews, Peggy J

2013-12-24T23:59:59.000Z

459

EE580 Solar Cells Todd J. Kaiser  

E-Print Network [OSTI]

7/21/2010 1 EE580 ­ Solar Cells Todd J. Kaiser · Lecture 10 · Summary 1Montana State University: Solar Cells Lecture 10: Summary Summer 2010 Class Montana State University: Solar Cells Lecture 10: Summary 2 Solar Cell Operation n Emitter p Base Rear Contact Antireflection coating Absorption of photon

Kaiser, Todd J.

460

(Melanin-Sensitized Solar Cell) : 696220016  

E-Print Network [OSTI]

the majority dye-sensitized solar cell research all uses the Ruthenium-complex as a light harvester. Dye-sensitized solar cell, DSSC 1991GrätzelDSSC[1] DSSCGrätzel cellDSSC polypyridyl complexes (Melanin-Sensitized Solar Cell) : : : 696220016 #12; #12;#12; #12;I PLD

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Directional Sensing Orients Cell Migration and Polarization  

E-Print Network [OSTI]

and interactions between the cells of our nervous system during development (9). Later in life, cell movements downstream pathways. In Dictyostelium, chemotaxis plays a critical role in all stages of its life cycle of the cell cortex (10, 46, 49, 55). In neutrophils and highly developed Dictyostelium cells, the efficiency

Devreotes, Peter

462

Mechanisms leading to electrically isolated cell  

E-Print Network [OSTI]

Mechanisms leading to electrically isolated cell parts and power loss under mechanical loads F cracks are omnipresent #12;Power loss by cell cracks Humidity freeze cycle 0 50 100 150 200 Powerloss, isolated cell area Crack mode: #12;Power loss after mechanical load · Some solar cells with mode A cracks

463

Molecular control of embryonic stem cell identity  

E-Print Network [OSTI]

Embryonic Stem (ES) cells are the in vitro derivatives of the inner cell mass of a developing embryo, and exhibit the property of pluripotency, which is the ability of a cell to give rise to all cell lineages of an organism. ...

Mathur, Divya, Ph. D. Massachusetts Institute of Technology

2008-01-01T23:59:59.000Z

464

DOE Fuel Cell Subprogram Nancy Garland  

E-Print Network [OSTI]

hydrogen fuel cell power system at a cost of $45/kW with 5000 hours of durability (80°C); by 2015, a cost a distributed generation PEM fuel cell system operating on natural gas or LPG that achieves 40% electricalDOE Fuel Cell Subprogram Nancy Garland Acting Fuel Cell Team Leader Pre-Solicitation Meeting Golden

465

NISTIR 7387 Cell Phone Forensic Tools  

E-Print Network [OSTI]

NISTIR 7387 Cell Phone Forensic Tools: AnOverviewandAnalysisUpdate RickAyers WayneJansen LudovicMoenner AurelienDelaitre #12;iii NISTIR 7387 Cell Phone Forensic Tools: An Overview and Analysis available for the purpose. iii #12;Abstract Cell phones and other handheld devices incorporating cell phone

466

NISTIR 7250 Cell Phone Forensic Tools  

E-Print Network [OSTI]

NISTIR 7250 Cell Phone Forensic Tools: AnOverviewandAnalysis RickAyers WayneJansen NicolasCilleros RonanDaniellou #12;iii NISTIR 7250 Cell Phone Forensic Tools: An Overview and Analysis Rick Ayers Wayne Interagency Report 187 pages (2005) iii #12;Abstract Cell phones and other handheld devices incorporating cell

467

FUEL CELL TECHNOLOGIES PROGRAM Safety, Codes, and  

E-Print Network [OSTI]

. Many odorants can also contaminate fuel cells. Hydrogen burns very quickly. Under optimal combustionFUEL CELL TECHNOLOGIES PROGRAM Safety, Codes, and Standards Hydrogen and fuel cell technologies, nuclear, natural gas, and coal with carbon sequestration. Fuel cells provide a highly efficient means

468

2008 FUEL CELL TECHNOLOGIES MARKET REPORT  

E-Print Network [OSTI]

2008 FUEL CELL TECHNOLOGIES MARKET REPORT JUNE 2010 #12;2008 FUEL CELL TECHNOLOGIES MARKET REPORT i and the fuel cell industry. The authors especially wish to thank Sunita Satyapal, Nancy Garland, and the staff of the U.S. Department of Energy's Fuel Cell Technologies Program for their support and guidance

469

Spectral sensitization of nanocrystalline solar cells  

DOE Patents [OSTI]

This invention relates to dye sensitized polycrystalline photoelectrochemical solar cells for use in energy transduction from light to electricity. It concerns the utility of highly absorbing organic chromophores as sensitizers in such cells and the degree to which they may be utilized alone and in combination to produce an efficient photoelectrochemical cell, e.g., a regenerative solar cell.

Spitler, Mark T. (Concord, MA); Ehret, Anne (Malden, MA); Stuhl, Louis S. (Bedford, MA)

2002-01-01T23:59:59.000Z

470

Corrugated Membrane Fuel Cell Structures  

SciTech Connect (OSTI)

One of the most challenging aspects of traditional PEM fuel cell stacks is the difficulty achieving the platinum catalyst utilization target of 0.2 gPt/kWe set forth by the DOE. Good catalyst utilization can be achieved with state-of-the-art catalyst coated membranes (CCM) when low catalyst loadings (<0.3 mg/cm2) are used at a low current. However, when low platinum loadings are used, the peak power density is lower than conventional loadings, requiring a larger total active area and a larger bipolar plate. This results in a lower overall stack power density not meeting the DOE target. By corrugating the fuel cell membrane electrode structure, Ion Power?s goal is to realize both the Pt utilization targets as well as the power density targets of the DOE. This will be achieved by demonstrating a fuel cell single cell (50 cm2) with a twofold increase in the membrane active area over the geometric area of the cell by corrugating the MEA structure. The corrugating structure must be able to demonstrate the target properties of < 10 mOhm-cm2 electrical resistance at > 20 psi compressive strength over the active area, in combination with offering at least 80% of power density that can be achieved by using the same MEA in a flat plate structure. Corrugated membrane fuel cell structures also have the potential to meet DOE power density targets by essentially packaging more membrane area into the same fuel cell volume as compared to conventional stack constructions.

Grot, Stephen [President, Ion Power Inc.] President, Ion Power Inc.

2013-09-30T23:59:59.000Z

471

Un-Nanostructuring Solar Cells | ANSER Center | Argonne-Northwestern...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Un-Nanostructuring Solar Cells Home > Research > ANSER Research Highlights > Un-Nanostructuring Solar Cells...

472

Effects of cell area on the performance of dye sensitized solar cell  

SciTech Connect (OSTI)

Dye sensitized solar cells (DSCs) have significant advantage over the current silicon cells by having low manufacturing cost and potentially high conversion efficiency. Therefore, DSCs are expected to be used as the next generation solar cell device that covers wide range of new applications. In order to achieve highly efficient DSCs for practical application, study on the effect of increasing the cell’s area on the performance of dye sensitized solar need to be carried out. Three different DSC cell areas namely, 1, 12.96 and 93.5 cm{sup 2} respectively were fabricated and analyzed through solar simulator and electrochemical impedance spectroscopy (EIS). From the analysis of electrochemical impedance spectroscopy (EIS), it was observed that the cell’s electron lifetime was influenced significantly by the cell’s area. Although the collection efficiency of all cells recorded to be approximately 100% but higher recombination rate with increased cell area reduced the performance of the cell.

Khatani, Mehboob, E-mail: mkhatani@hotmail.com, E-mail: noranimuti-mohamed@petronas.com.my, E-mail: hishmid@petronas.com.my, E-mail: azclement@yahoo.com, E-mail: aeska07@gmail.com; Mohamed, Norani Muti, E-mail: mkhatani@hotmail.com, E-mail: noranimuti-mohamed@petronas.com.my, E-mail: hishmid@petronas.com.my, E-mail: azclement@yahoo.com, E-mail: aeska07@gmail.com; Hamid, Nor Hisham, E-mail: mkhatani@hotmail.com, E-mail: noranimuti-mohamed@petronas.com.my, E-mail: hishmid@petronas.com.my, E-mail: azclement@yahoo.com, E-mail: aeska07@gmail.com; Sahmer, Ahmad Zahrin, E-mail: mkhatani@hotmail.com, E-mail: noranimuti-mohamed@petronas.com.my, E-mail: hishmid@petronas.com.my, E-mail: azclement@yahoo.com, E-mail: aeska07@gmail.com; Samsudin, Adel, E-mail: mkhatani@hotmail.com, E-mail: noranimuti-mohamed@petronas.com.my, E-mail: hishmid@petronas.com.my, E-mail: azclement@yahoo.com, E-mail: aeska07@gmail.com [Centre of Innovative Nanostructures and Nanodevices (COINN), UTP (Malaysia)

2014-10-24T23:59:59.000Z

473

Do Cell Phones Cause Cancer?  

E-Print Network [OSTI]

Do cell phones, household electrical power wiring or appliance, or high voltage power lines cause cancer? Fuggedaboudit! No way! When pigs fly! When I'm the Pope! Don't text while you're driving, however, or eat your cell phone. All organisms absorb microwave radiation directly as thermal energy. In living organisms, the organisms' thermal control systems, including the blood flow, and various cooling mechanisms, such as sweating in humans, that work to maintain a stable body temperature rapidly transfer the absorbed energy to the environment. Any temperature rise is small or even unobserved. Any proposed mechanism by which cell phone radiation might cause cancer must begin with this fact. But the amount of radiation absorbed from a cell phone is less than that produced by normal metabolic processes, and much less than that produced by, for example, exercise. None of these normal metabolic processes cause cancer. Therefore, the much smaller amounts of energy from cell phones doesn't cause cancer either. All f...

Leikind, Bernard

2010-01-01T23:59:59.000Z

474

Capillary reference half-cell  

DOE Patents [OSTI]

The present invention is a reference half-cell electrode wherein intermingling of test fluid with reference fluid does not affect the performance of the reference half-cell over a long time. This intermingling reference half-cell may be used as a single or double junction submersible or surface reference electrode. The intermingling reference half-cell relies on a capillary tube having a first end open to reference fluid and a second end open to test fluid wherein the small diameter of the capillary tube limits free motion of fluid within the capillary to diffusion. The electrode is placed near the first end of the capillary in contact with the reference fluid. The method of operation of the present invention begins with filling the capillary tube with a reference solution. After closing the first end of the capillary, the capillary tube may be fully submerged or partially submerged with the second open end inserted into test fluid. Since the electrode is placed near the first end of the capillary, and since the test fluid may intermingle with the reference fluid through the second open end only by diffusion, this intermingling capillary reference half-cell provides a stable voltage potential for long time periods. 11 figs.

Hall, S.H.

1996-02-13T23:59:59.000Z

475

Current and lattice matched tandem solar cell  

DOE Patents [OSTI]

A multijunction (cascade) tandem photovoltaic solar cell device is fabricated of a Ga.sub.x In.sub.1-x P (0.505.ltoreq.X.ltoreq.0.515) top cell semiconductor lattice matched to a GaAs bottom cell semiconductor at a low-resistance heterojunction, preferably a p+/n+ heterojunction between the cells. The top and bottom cells are both lattice matched and current matched for high efficiency solar radiation conversion to electrical energy.

Olson, Jerry M. (Lakewood, CO)

1987-01-01T23:59:59.000Z

476

1990 fuel cell seminar: Program and abstracts  

SciTech Connect (OSTI)

This volume contains author prepared short resumes of the presentations at the 1990 Fuel Cell Seminar held November 25-28, 1990 in Phoenix, Arizona. Contained herein are 134 short descriptions organized into topic areas entitled An Environmental Overview, Transportation Applications, Technology Advancements for Molten Carbonate Fuel Cells, Technology Advancements for Solid Fuel Cells, Component Technologies and Systems Analysis, Stationary Power Applications, Marine and Space Applications, Technology Advancements for Acid Type Fuel Cells, and Technology Advancement for Solid Oxide Fuel Cells.

Not Available

1990-12-31T23:59:59.000Z

477

Fuel Cell R&D Activities | Department of Energy  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

Fuel Cell R&D Activities Fuel Cell R&D Activities Photo of electric motor under the hood of fuel cell car The Fuel Cell Technologies fuel cell research and development (R&D)...

478

Fuel Cell Technologies Office Multi-Year Research, Development...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

3.4 Fuel Cells Fuel Cell Technologies Office Multi-Year Research, Development, and Demonstration Plan - 3.4 Fuel Cells Fuel Cells technical plan section of the Fuel Cell...

479

Cell Host & Microbe Translocation of Sickle Cell Erythrocyte MicroRNAs  

E-Print Network [OSTI]

Cell Host & Microbe Article Translocation of Sickle Cell Erythrocyte MicroRNAs into Plasmodium a variant hemoglobin allele (HbS), which causes sickle cell disease and resists infection by the malaria in translational inhibition. Hence, sickle cell erythrocytes exhibit cell-intrinsic resistance to malaria in part

Nicchitta, Chris

480

Investigation of Fuel Cell System Performance and Operation: A Fuel Cell as a Practical  

E-Print Network [OSTI]

Investigation of Fuel Cell System Performance and Operation: A Fuel Cell as a Practical Distributed of Fuel Cell System Performance and Operation: A Fuel Cell as a Practical Distributed Generator George Research Center program. This report is of work done under the PSERC project "Investigation of Fuel Cell

Note: This page contains sample records for the topic "mammary epithelial cells" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Care and Feeding of mAb104 cells To thaw cells  

E-Print Network [OSTI]

antibody stock). Also can add 0.1% sodium azide. (Discard cells that will be sick at this point). To Freeze. Spin down cells in sterile conical tubes. 5 min at 1 K. Aspirate supernatant. 3. Resuspend cells in 1Care and Feeding of mAb104 cells To thaw cells: 1. Thaw bullet quickly (in hand or water bath

Lynch, Kristen W.

482

USCAR FUEL CELL TECH TEAM CELL COMPONENT ACCELERATED STRESS TEST PROTOCOLS  

E-Print Network [OSTI]

USCAR FUEL CELL TECH TEAM CELL COMPONENT ACCELERATED STRESS TEST PROTOCOLS FOR PEM FUEL CELLS of polymer electrolyte membrane (PEM) fuel cell components under simulated automotive drive cycle conditions of PEM fuel cells. Corrosion of high-surface area carbon supports poses significant concerns at high

483

1 | Fuel Cell Technologies Office eere.energy.gov DOE Fuel Cell Technologies Office  

E-Print Network [OSTI]

Storage Engineering Center of Excellence 2013 ·H2USA Launch DOE Fuel Cell Technologies ­ Recent History1 | Fuel Cell Technologies Office eere.energy.gov DOE Fuel Cell Technologies Office Fuel Cell Seminar & Energy Exposition Columbus, Ohio Dr. Sunita Satyapal Director Fuel Cell Technologies Office

484

1 | Fuel Cell Technologies Program eere.energy.gov Fuel Cell Technologies Program  

E-Print Network [OSTI]

, and Specialty Vehicles Fuel cells can be a cost-competitive option for critical-load facilities, backup power1 | Fuel Cell Technologies Program eere.energy.gov Fuel Cell Technologies Program DOE Hydrogen & Fuel Cell Overview Dr. Sunita Satyapal Program Manager U.S. Department of Energy Fuel Cell Technologies

485

Development of alkaline fuel cells.  

SciTech Connect (OSTI)

This project focuses on the development and demonstration of anion exchange membrane (AEM) fuel cells for portable power applications. Novel polymeric anion exchange membranes and ionomers with high chemical stabilities were prepared characterized by researchers at Sandia National Laboratories. Durable, non-precious metal catalysts were prepared by Dr. Plamen Atanassov's research group at the University of New Mexico by utilizing an aerosol-based process to prepare templated nano-structures. Dr. Andy Herring's group at the Colorado School of Mines combined all of these materials to fabricate and test membrane electrode assemblies for single cell testing in a methanol-fueled alkaline system. The highest power density achieved in this study was 54 mW/cm2 which was 90% of the project target and the highest reported power density for a direct methanol alkaline fuel cell.

Hibbs, Michael R.; Jenkins, Janelle E.; Alam, Todd Michael; Janarthanan, Rajeswari [Colorado School of Mines, Golden, CO; Horan, James L. [Colorado School of Mines, Golden, CO; Caire, Benjamin R. [Colorado School of Mines, Golden, CO; Ziegler, Zachary C. [Colorado School of Mines, Golden, CO; Herring, Andrew M. [Colorado School of Mines, Golden, CO; Yang, Yuan [Colorado School of Mines, Golden, CO; Zuo, Xiaobing [Argonne National Laboratory, Argonne, IL; Robson, Michael H. [University of New Mexico, Albuquerque, NM; Artyushkova, Kateryna [University of New Mexico, Albuquerque, NM; Patterson, Wendy [University of New Mexico, Albuquerque, NM; Atanassov, Plamen Borissov [University of New Mexico, Albuquerque, NM

2013-09-01T23:59:59.000Z

486

Development of concentrator solar cells  

SciTech Connect (OSTI)

A limited pilot production run on PESC silicon solar cells for use at high concentrations (200 to 400 suns) is summarized. The front contact design of the cells was modified for operation without prismatic covers. The original objective of the contract was to systematically complete a process consolidation phase, in which all the, process improvements developed during the contract would be combined in a pilot production run. This pilot run was going to provide, a basis for estimating cell costs when produced at high throughput. Because of DOE funding limitations, the Photovoltaic Concentrator Initiative is on hold, and Applied Solar`s contract was operated at a low level of effort for most of 1993. The results obtained from the reduced scope pilot run showed the effects of discontinuous process optimization and characterization. However, the run provided valuable insight into the technical areas that can be optimized to achieve the original goals of the contract.

Not Available

1994-08-01T23:59:59.000Z

487

Bulk Modulus Capacitor Load Cells  

SciTech Connect (OSTI)

Measurement of forces present at various locations within the SSC Model Dipole collared coil assembly is of great practical interest to development engineers. Of particular interest are the forces between coils at the parting plane and forces that exist between coils and pole pieces. It is also desired to observe these forces under the various conditions that a magnet will experience such as: during the collaring process, post-collaring, under the influence of cryogens, and during field excitation. A twenty eight thousandths of an inch thick capacitor load cell which utilizes the hydrostatic condition of a stressed plastic dielectric has been designed. These cells are currently being installed on SSC Model Dipoles. The theory, development, and application of these cells will be discussed.

Dickey, C.E.

1990-04-01T23:59:59.000Z

488

Fuel Cell Applied Research Project  

SciTech Connect (OSTI)

Since November 12, 2003, Northern Alberta Institute of Technology has been operating a 200 kW phosphoric acid fuel cell to provide electrical and thermal energy to its campus. The project was made possible by funding from the U.S. Department of Energy as well as by a partnership with the provincial Alberta Energy Research Institute; a private-public partnership, Climate Change Central; the federal Ministry of Western Economic Development; and local natural gas supplier, ATCO Gas. Operation of the fuel cell has contributed to reducing NAIT's carbon dioxide emissions through its efficient use of natural gas.

Lee Richardson

2006-09-15T23:59:59.000Z

489

Three-junction solar cell  

DOE Patents [OSTI]

A photovoltaic solar cell is formed in a monolithic semiconductor. The cell contains three junctions. In sequence from the light-entering face, the junctions have a high, a medium, and a low energy gap. The lower junctions are connected in series by one or more metallic members connecting the top of the lower junction through apertures to the bottom of the middle junction. The upper junction is connected in voltage opposition to the lower and middle junctions by second metallic electrodes deposited in holes 60 through the upper junction. The second electrodes are connected to an external terminal.

Ludowise, Michael J. (Cupertino, CA)

1986-01-01T23:59:59.000Z

490

DIGESTER GAS - FUEL CELL - PROJECT  

SciTech Connect (OSTI)

GEW has been operating the first fuel cell in Europe producing heat and electricity from digester gas in an environmentally friendly way. The first 9,000 hours in operation were successfully concluded in August 2001. The fuel cell powered by digester gas was one of the 25 registered ''Worldwide projects'' which NRW presented at the EXPO 2000. In addition to this, it is a key project of the NRW State Initiative on Future Energies. All of the activities planned for the first year of operation were successfully completed: installing and putting the plant into operation, the transition to permanent operation as well as extended monitoring till May 2001.

Dr.-Eng. Dirk Adolph; Dipl.-Eng. Thomas Saure

2002-03-01T23:59:59.000Z

491

Superlattice photoelectrodes for photoelectrochemical cells  

DOE Patents [OSTI]

The application of superlattice semiconductors as photoelectrodes in photoelectrochemical energy conversion processes is described. The invention is comprised of a multiple quantum well, or superlattice, semiconductor positioned on a plate and encapsulated in an insulation material, except the top surface, which is left exposed. An opening in insulation exposes a portion of the plate. When the photoelectrochemical cell is immersed in a liquid electrolyte and exposed to solar radiation, a redox reaction occurs, producing gases such as hydrogen and oxygen from a water electrolyte, which bubble off the cathode and anode portions of the cell. (LEW)

Nozik, A.J.

1985-07-03T23:59:59.000Z

492

Sandia National Laboratories: Fuel Cells  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administration the1 -theErik Spoerke SSLS Exhibit at Explora MuseumFloatingFront EdgeCells Fuel Cells On

493

Fuel cell technology for prototype logistic fuel cell mobile systems  

SciTech Connect (OSTI)

Under the aegis of the Advanced Research Project Agency`s family of programs to develop advanced technology for dual use applications, International Fuel Cells Corporation (IFC) is conducting a 39 month program to develop an innovative system concept for DoD Mobile Electric Power (MEP) applications. The concept is to integrate two technologies, the phosphoric acid fuel cell (PAFC) with an auto-thermal reformer (ATR), into an efficient fuel cell power plant of nominally 100-kilowatt rating which operates on logistic fuels (JP-8). The ATR fuel processor is the key to meeting requirements for MEP (including weight, volume, reliability, maintainability, efficiency, and especially operation on logistic fuels); most of the effort is devoted to ATR development. An integrated demonstration test unit culminates the program and displays the benefits of the fuel cell system, relative to the standard 100-kilowatt MEP diesel engine generator set. A successful test provides the basis for proceeding toward deployment. This paper describes the results of the first twelve months of activity during which specific program aims have remained firm.

Sederquist, R.A.; Garow, J.

1995-08-01T23:59:59.000Z

494

Cell Surface Conjugation of Sialyl Lewis X Induces a Rolling Response for Mesenchymal Stem Cells  

E-Print Network [OSTI]

There has been significant interest in the clinical use of adult mesenchymal stem cells (MSCs), which are connective tissue progenitor cells. One of the greatest challenges in traditional stem cell therapy is to deliver a ...

Karp, Jeffrey Michael

495

Optimization of Fuel Cell System Operating Conditions for Fuel Cell Vehicles  

E-Print Network [OSTI]

An Indirect Methanol Pem Fuel Cell System, SAE 2001, (paperof automotive PEM fuel cell stacks, SAE 2000 (paper number1009). for an automotive PEM fuel cell system with imbedded

Zhao, Hengbing; Burke, Andy

2008-01-01T23:59:59.000Z

496

Webinar: California Fuel Cell Partnership's Roadmap to the Commercialization of Hydrogen Fuel Cell Electric Vehicles  

Broader source: Energy.gov [DOE]

Video recording of the Fuel Cell Technologies Office webinar, California Fuel Cell Partnership's Roadmap to the Commercialization of Hydrogen Fuel Cell Electric Vehicles, originally presented on October 16, 2013.

497

Questions about Embryonic Stem Cells Where do ES cells come from?  

E-Print Network [OSTI]

are not destroyed every time new research is performed. · ES cells do not come from aborted fetuses. How could cause problems if used in patients. What are ES cells used for? · ES cells

498

A Review of Gene Delivery and Stem Cell Based Therapies for Regenerating Inner Ear Hair Cells  

E-Print Network [OSTI]

regenerative capacity of the inner ear hair cells. With recent advances in understanding the developmental biology of mammalian and non-mammalian hair cells a variety of strategies have emerged to restore lost hair cells are being developed. Two predominant...

Devarajan, Keerthana; Staecker, Hinrich; Detamore, Michael S.

2011-09-13T23:59:59.000Z

499

Gas concentration cells for utilizing energy  

DOE Patents [OSTI]

An apparatus and method are disclosed for utilizing energy, in which the apparatus may be used for generating electricity or as a heat pump. When used as an electrical generator, two gas concentration cells are connected in a closed gas circuit. The first gas concentration cell is heated and generates electricity. The second gas concentration cell repressurizes the gas which travels between the cells. The electrical energy which is generated by the first cell drives the second cell as well as an electrical load. When used as a heat pump, two gas concentration cells are connected in a closed gas circuit. The first cell is supplied with electrical energy from a direct current source and releases heat. The second cell absorbs heat. The apparatus has no moving parts and thus approximates a heat engine. 4 figs.

Salomon, R.E.

1987-06-30T23:59:59.000Z

500

Gas concentration cells for utilizing energy  

DOE Patents [OSTI]

An apparatus and method for utilizing energy, in which the apparatus may be used for generating electricity or as a heat pump. When used as an electrical generator, two gas concentration cells are connected in a closed gas circuit. The first gas concentration cell is heated and generates electricity. The second gas concentration cell repressurizes the gas which travels between the cells. The electrical energy which is generated by the first cell drives the second cell as well as an electrical load. When used as a heat pump, two gas concentration cells are connected in a closed gas circuit. The first cell is supplied with electrical energy from a direct current source and releases heat. The second cell absorbs heat. The apparatus has no moving parts and thus approximates a heat engine.

Salomon, Robert E. (Philadelphia, PA)

1987-01-01T23:59:59.000Z