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1

Functional Proteomic Pattern Identification under Low Dose Ionizing Radiation  

Science Conference Proceedings (OSTI)

The goal of this study is to explore and to understand the dynamic responses of signaling pathways to low dose ionizing radiation (IR). Low dose radiation (10 cGy or lower) affects several signaling pathways including DNA repair, survival, cell cycle, ... Keywords: low dose radiation, functional proteomics

Young Bun Kim; Jean Gao; Ying Dong; Chin-Rang Yang

2008-11-01T23:59:59.000Z

2

Low Dose Radiation Research Program: Low Dose Ionizing Radiation-Induced  

NLE Websites -- All DOE Office Websites (Extended Search)

Low Dose Ionizing Radiation-Induced Effects in Irradiated and Low Dose Ionizing Radiation-Induced Effects in Irradiated and Unirradiated cells: Pathways Analysis in Support of Risk Assessment. Authors: B.E. Lehnert, R. Cary, D. Gadbois, and G. Gupta. Institutions: Bioscience Division, Los Alamos National Laboratory. The scientific literature presents a confusing picture concerning health risks due to low dose ionizing radiation (LDIR), e.g., <1-10 cGy. Some effects of LDIR such as enhanced rates of cell proliferation and the induction of radioadaptation may be benign under some circumstances. Other evidence suggests LDIR can be hazardous and that a threshold for potentially detrimental responses, e.g., increases in intracellular reactive oxygen species (ROS), increases in sister chromatid exchanges (SCE), alterations in gene or protein expression profiles, and increased

3

Oxidative Stress and Skeletal Health with Low Dose, Ionizing Radiation  

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Oxidative Stress and Skeletal Health with Low Dose, Ionizing Radiation Oxidative Stress and Skeletal Health with Low Dose, Ionizing Radiation Globus Ruth NASA Ames Research Center Abstract Osteoporosis profoundly affects the aging U.S. population and exposure to high doses of radiation causes bone loss similar to age-related osteoporosis, although the influence of low dose radiation exposures is not known. The central hypothesis of our DOE project (NASA supplement) is that low doses of radiation modulate subsequent skeletal degeneration via oxidative pathways. Our working hypothesis is that a prior exposure to low dose radiation regulates oxidative metabolism within bone and contributes to bone loss caused either by subsequent high, challenge doses of radiation or by aging. HZE source: Because astronauts are exposed to radiation from GCR and solar

4

Low dose ionizing radiation induces tumor growth promoting factors in  

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ionizing radiation induces tumor growth promoting factors in ionizing radiation induces tumor growth promoting factors in stress-induced premature senescent fibroblasts David Boothman University of Texas Southwestern Medical Center at Dallas Abstract Recent evidence suggest that the causes of cancer development are not limited to mutations within cancer cells, but also involve in alterations of cancer microenvironment. Senescent cells are irreversibly growth arrested, but remain metabolically active. Senescent cells, especially senescent fibroblasts in the stroma may provide a beneficial environment for tumor growth through secretion of certain factors. Accumulation of senescent cells in the stroma of patients repeatedly exposed to low doses of IR or low dose rates of IR, could be an important factor, causing alteration of the microenvironment that ultimately benefits tumor

5

Low Dose Radiation Research Program: Research Highlights - Ionizing...  

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of Energy Low Dose Radiation Research Program, and the Department of Defense Breast Cancer Research program and Prostate Cancer Research program. Researchers include Eva...

6

Investigation of non-targeted effects of low dose ionizing radiation...  

NLE Websites -- All DOE Office Websites (Extended Search)

Investigation of non-targeted effects of low dose ionizing radiation on the mammary gland utilizing three-dimensional culture models of mammary cells derived from mouse strains...

7

Low Dose Radiation Research Program: Research Highlights - Ionizing  

NLE Websites -- All DOE Office Websites (Extended Search)

Affects Cancer Frequency and Characteristics by Acting Affects Cancer Frequency and Characteristics by Acting on the Microenvironment Background: For more than a quarter century the scientific rationale for extrapolating radiation health effects has been underpinned by biophysical target theory. Fundamental to target theory is that the effect (e.g., DNA damage, mutation, cancer) is proportional to dose based on interaction of energy with biological targets, specifically DNA. However, the biology following ionizing radiation is more than just DNA damage, repair, or misrepair. Cellular responses to ionizing radiation can affect phenotype, cell interactions, lineage commitment, differentiation and genomic stability, all of which have been widely documented in cultured cells and many observed in vivo. This class of non-targeted effects induced

8

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary  

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Genetic susceptibility to low-dose ionizing radiation in the mouse mammary Genetic susceptibility to low-dose ionizing radiation in the mouse mammary gland as a means of understanding human risk for breast cancer Antoine M. Snijders Lawrence Berkeley National Laboratory Abstract Goal: Our goal is to develop an in vivo mechanistic model of genetic variation in the low-dose damage responses of mammary glands using inbred mice known to vary in their sensitivity to low-dose induced mammary gland cancer, and to develop molecular predictors for susceptibility or resistance to low-dose induced breast cancer. Background and Significance: It is increasingly believed that individuals differ in their genetic susceptibilities to environmental insults for diseases such as cancer. This concern is especially important for the large numbers of individuals receiving low-dose exposures in the nuclear energy

9

Low dose ionizing radiation (IR) signaling regulation in vivo...  

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studies from our lab indicated that human cells exposed to low doses of IR caused growth stimulation, speeding cells up in their cell cycle division (i.e., checkpoint regulation),...

10

Low Dose Ionizing Radiation Modulates Immune Function New Project Overview  

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Modulates Immune Function New Project Overview Modulates Immune Function New Project Overview Gregory Nelson Loma Linda University Abstract The immune system provides the first line of defense for exposures to environmental hazards. Protective immunity mechanisms using innate or adaptive responses are employed to mitigate acute challenges or amplify the readiness of the system to respond to future challenges. Some stimuli lead to amplified inflammatory reactions such as delayed hypersensitivity which is required for immunity to parasites and can also lead to adverse consequences such as contact dermatitis. Radiation exposure has the potential to aggravate hypersensitivity reactions as well as to suppress protective immunity. Ionizing radiation at high doses has long been recognized as highly effective in destroying cells of the immune system,

11

Investigation of non-targeted effects of low dose ionizing radiation on the mammary gland  

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non-targeted effects of low dose ionizing radiation on the mammary gland non-targeted effects of low dose ionizing radiation on the mammary gland utilizing three-dimensional culture models of mammary cells derived from mouse strains that differ in susceptibility to tumorigenesis Joni D. Mott, Antoine M. Snijders, Alvin Lo, Dinah Levy-Groesser, Bahram Parvin, Andrew J. Wyrobek, Jian-Hua Mao, and Mina J. Bissell Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley CA 94720 Goal: Within the Lawrence Berkeley National Laboratory's SFA, Project 2, our studies focus on utilizing three dimensional (3D) cell culture models as surrogates for in vivo studies to determine how low doses of ionizing radiation influence mammary gland tissue architecture and how this may relate both to tumor progression and/or adaptive response.

12

Mechanisms Underlying Cellular Responses to Low Doses/Low LET Ionizing Radiation in Primary Haemopoietic Cells.  

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Mechanisms Underlying Cellular Responses to Low Doses/Low LET Ionizing Radiation Mechanisms Underlying Cellular Responses to Low Doses/Low LET Ionizing Radiation in Primary Haemopoietic Cells. Munira Kadhim 1 , Stefania Militi 1 , Debbie Bowler 1 , Denise Macdonald 1 and Kevin Prise 2 1 Radiation and Genome Stability Unit, MRC, Harwell, Didcot, Oxon, OX11 0RD, UK 2 Gray Cancer Institute ,PO Box 100, Mount Vernon Hospital, Northwood, HA6 2JR, UK Because the human population is genetically heterogeneous, it is important to understand the role that heterogeneity may play in radiation response. Exposure to ionizing radiation can lead to a suite of changes, including increased mutation rate, delayed reproductive cell death, and delayed chromosomal aberrations, all of which are manifestations of the complex genomic instability (GI) phenotype. Following exposure to either high LET

13

Proteomic and Biochemical Studies of Human Mesenchymal Stem Cells in Response to Low Dose Ionizing Radiation  

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Proteomic and Biochemical Studies of Human Mesenchymal Stem Cells Proteomic and Biochemical Studies of Human Mesenchymal Stem Cells in Response to Low Dose Ionizing Radiation Deok-Jin Jang 1 , Mingquan Guo 1 , Julia S.F.Chu 2 , Kyle T. Kurpinski 2 , Bjorn Rydberg 1 , Song Li 2 , and Daojing Wang 1 1. Life Sciences Division, Lawrence Berkeley National Lab, Berkeley, CA 94720 2. Department of Bioengineering, University of California, Berkeley, CA 94720 We will present data obtained during the first year of our DOE/NASA Low Dose Radiation Research program. We utilized a comprehensive approach including transcriptomics, proteomics, phosphoproteomics, and biochemistry to characterize human mesenchymal stem cells (MSCs) in response to low dose ionizing radiation. We first determined the cell survival, proliferation, and osteogenic differentiation of

14

Mammalian Tissue Response to Low Dose Ionizing Radiation: The Role of Oxidative Metabolism and Intercellular Communication  

SciTech Connect

The objective of the project was to elucidate the mechanisms underlying the biological effects of low dose/low dose rate ionizing radiation in organs/tissues of irradiated mice that differ in their susceptibility to ionizing radiation, and in human cells grown under conditions that mimic the natural in vivo environment. The focus was on the effects of sparsely ionizing cesium-137 gamma rays and the role of oxidative metabolism and intercellular communication in these effects. Four Specific Aims were proposed. The integrated outcome of the experiments performed to investigate these aims has been significant towards developing a scientific basis to more accurately estimate human health risks from exposures to low doses ionizing radiation. By understanding the biochemical and molecular changes induced by low dose radiation, several novel markers associated with mitochondrial functions were identified, which has opened new avenues to investigate metabolic processes that may be affected by such exposure. In particular, a sensitive biomarker that is differentially modulated by low and high dose gamma rays was discovered.

Azzam, Edouard I

2013-01-16T23:59:59.000Z

15

Low-Dose Ionizing Radiation Alters the Epigenome of the Avy Mouse  

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Ionizing Radiation Alters the Epigenome of the A Ionizing Radiation Alters the Epigenome of the A vy Mouse Autumn Bernal 1,2,3 , Dale Huang 1 , Yue Li 4 , Dana Dolinoy 5 , and Randy Jirtle 1 Department of Radiation Oncology 1 , University Program in Genetics and Genomic 2 , Integrated Toxicology & Environmental Health Program 3 , Department of Community and Family Medicine 4 , Duke University Medical Center, Durham, NC, USA, Department of Environmental and Health Sciences, University of Michigan, Ann Arbor, MI, USA 4 Background: Humans have evolved and thrived amidst constant low-dose (0-10 cGy) background radiation exposure from natural sources. Currently, however, the frequency of exposures to low doses of radiation is increasing due to man-made sources such as diagnostic imaging and nuclear power. This increased exposure has led to concerns amongst the general public and the government about the

16

Metabolomic Response of Human Skin Tissue to Low Dose Ionizing Radiation  

Science Conference Proceedings (OSTI)

Understanding how human organs respond to ionizing radiation (IR) at a systems biology level and identifying biomarkers for IR exposure at low doses can help provide a scientific basis for establishing radiation protection standards. Little is known regarding the physiological responses to low dose IR at the metabolite level, which represents the end-point of biochemical processes inside cells. Using a full thickness human skin tissue model and GC-MS-based metabolomics analysis, we examined the metabolic perturbations at three time points (3, 24 and 48 hr) after exposure to 3, 10 and 200 cGy of X-rays. PLS-DA score plots revealed dose- and time-dependent clustering between sham and irradiated groups. Importantly, a comparable number of metabolites were detected to have significant change 48 hr after exposure to 3 and 10 cGy of irradiation, when compared with the high dose of 200 cGy. Biochemical pathway analysis showed perturbations to DNA/RNA damage and repair, lipid and energy metabolisms, even at low doses of IR.

Hu, Zeping; Kim, Young-Mo; Sowa, Marianne B.; Robinson, Robert J.; Gao, Xiaoli; Metz, Thomas O.; Morgan, William F.; Zhang, Qibin

2012-05-18T23:59:59.000Z

17

Mitochondrial-Derived Oxidants and Cellular Responses to Low Dose/Low LET Ionizing Radiation  

SciTech Connect

Exposure to ionizing radiation results in the immediate formation of free radicals and other reactive oxygen species (ROS). It has been assumed that the subsequent injury processes leading to genomic instability and carcinogenesis following radiation, derive from the initial oxidative damage caused by these free radicals and ROS. It is now becoming increasingly obvious that metabolic oxidation/reduction (redox) reactions can be altered by irradiation leading to persistent increases in steady-state levels of intracellular free radicals and ROS that contribute to the long term biological effects of radiation exposure by causing chronic oxidative stress. The objective during the last period of support (DE-FG02-05ER64050; 5/15/05-12/31/09) was to determine the involvement of mitochondrial genetic defects in metabolic oxidative stress and the biological effects of low dose/low LET radiation. Aim 1 was to determine if cells with mutations in succinate dehydrogenase (SDH) subunits C and D (SDHC and SDHD in mitochondrial complex II) demonstrated increases in steady-state levels of reactive oxygen species (ROS; O2- and H2O2) as well as demonstrating increased sensitivity to low dose/low LET radiation (10 cGy) in cultured mammalian cells. Aim #2 was to determine if mitochondrially-derived ROS contributed to increased sensitivity to low dose/low LET radiation in mammalian cells containing mutations in SDH subunits. Aim #3 was to determine if a causal relationship existed between increases in mitochondrial ROS production, alterations in electron transport chain proteins, and genomic instability in the progeny of irradiated cells. Evidence gathered in the 2005-2009 period of support demonstrated that mutations in genes coding for mitochondrial electron transport chain proteins (ETC); either Succinate Dehydrogenase (SDH) subunit C (SDHC) or subunit D (SDHD); caused increased ROS production, increased genomic instability, and increased sensitivity to low dose/low LET radiation that could be mitigated by over expression of the H2O2 metabolizing enzyme, catalase, and/or the mitochondrial form of superoxide dismutase (MnSOD). Furthermore, using radiation-induced genomically unstable cells, it was shown that steady-state levels of H2O2 were significantly elevated for many cell generations following exposure, catalase suppressed the radiation-induced mutator phenotype when added long after radiation exposure, unstable clones showed evidence of mitochondrial dysfunction some of which was characterized by improper assembly of SDH subunits (particularly subunit B), and chemical inhibitors of SDH activity could decrease steady-state levels of H2O2 as well as mutation frequency. These results support the hypotheses that 1) SDH mutations could contribute to transformation by inducing genomic instability and a mutator phenotype via increasing steady-state levels of ROS; 2) metabolic sources of O2- and H2O2 play a significant role in low dose radiation induced injury and genomic instability; and 3) increased mutation rates in irradiated mammal cells can be suppressed by scavengers of H2O2 (particularly catalase) long after radiation exposure. Overall the results obtained during this period of support provide clear evidence in support of the hypothesis that abnormal oxidative metabolism in mitochondria that result in increases in steady-sate levels of H2O2 and other ROS are capable of significantly contributing to radiation-induced mutator phenotypes in mammalian cells.

Spitz, Douglas R.

2009-11-09T23:59:59.000Z

18

Whole Genome Analysis of Functional Protein Binding Sites and DNA Methylation: Application to p53 and Low Dose Ionizing Radiation.  

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Whole Genome Analysis of Functional Protein Binding Sites and DNA Methylation: Whole Genome Analysis of Functional Protein Binding Sites and DNA Methylation: Application to p53 and Low Dose Ionizing Radiation. Krassimira Botcheva, John J. Dunn and Carl W. Anderson Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA The effects of exposure to low doses of ionizing radiation on humans results largely from changes in gene expression mediated by the activation of sequence-specific DNA binding proteins (transcription factors) as well as changes to other chromosomal proteins and perhaps to DNA. To develop a molecular understanding of the consequences of exposures to low doses of ionizing radiation, it will be necessary to understanding where radiation-activated transcription factors bind in whole genomes and how

19

Low-Dose Ionizing Radiation Alters the Epigenome of the Avy Mouse  

NLE Websites -- All DOE Office Websites (Extended Search)

Medical Center Abstract Background: Humans have evolved and thrived amidst constant low-dose (0-10 cGy) background radiation exposure from natural sources. Currently, however, the...

20

Induction of nuclear factor kB after low-dose ionizing radiation involves a reactive oxygen intermediate signaling pathway  

Science Conference Proceedings (OSTI)

Reactive oxygen intermediates (ROIs) have been found to be the messengers in the activation of the kB transcription regulator in mitogen- or cytokine-stimulated cells, operating in conjunction with or independently of various other mechanisms; these include Ca{sup ++}-dependent and PKC-dependent cytoplasmic signaling pathways. We have recently reported that low-dose ionizing radiation induces NF-kB in human lymphoblastoid 244B cells. Since ionizing radiation generates free radicals in cells, we have investigated whether the ROIs generated by ionizing radiation induce NF-kB activity, and also whether they do so by a similar mechanism as in cells treated with PMA or H{sub 2}O{sub 2}. The results not only confirm a previous observation from our laboratory that low-dose ionizing radiation (0.1-2.0 Gy) activates kB transcription factor transiently with a maximal induction at 0.5 Gy exposure, but also demonstrate mechanistically that the activation of NF-kB by low-dose ionizing radiation can be inhibited considerably by the antioxidant N-acetyl-L-cysteine, indicating that at least the major part of the activation process is mediated by ROIs. These findings support the idea that ROIs can regulate the kB elements which in turn can serve as response elements for oxidant stress. 37 refs., 4 figs., 1 tab.

Mohan, N.; Meltz, M.L. [Univ. of Texas Health Science Center, San Antonio, TX (United States)

1994-10-01T23:59:59.000Z

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Low Dose Radiation  

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Ancient Salt Beds Ancient Salt Beds Repository Science Renewable Energy The WIPP Underground may be ideal to study effects of Very Low Dose Rates on Biological Systems Low Background Radiation Experiment We're all bathing in it. It's in the food we eat, the water we drink, the soil we tread and even the air we breathe. It's background radiation, it's everywhere and we can't get away from it. But what would happen if you somehow "pulled the plug" on natural background radiation? Would organisms suffer or thrive if they grew up without their constant exposure to background radiation? That's what a consortium of scientists conducting an experiment at the Waste Isolation Pilot Plant aim to find out. Despite being an underground repository for transuranic radioactive waste,

22

Using Co-Regulation to Understand Low-Dose Ionizing Radiation...  

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with low and high doses of ionizing radiation (IR). Pathway analysis suggested that chromatin structure, as well as transcription control, plays a large role in linking gene...

23

Low Dose Radiation Research Program: Depletion of the Vertebrate...  

NLE Websites -- All DOE Office Websites (Extended Search)

Laboratory, Berkeley, California To better understand the responses to low dose ionizing radiation, we proposed in Aim 1 of our Low Dose grant to use dominant-negative...

24

Irradiators for measuring the biological effects of low dose-rate ionizing radiation fields  

E-Print Network (OSTI)

Biological response to ionizing radiation differs with radiation field. Particle type, energy spectrum, and dose-rate all affect biological response per unit dose. This thesis describes methods of spectral analysis, ...

Davidson, Matthew Allen

2011-01-01T23:59:59.000Z

25

Genome Wide Evaluation of Normal Human Tissue in Response to Controlled, In vivo Low-Dose Low LET Ionizing Radiation Exposure: Pathways and Mechanisms Final Report, September 2013  

SciTech Connect

During course of this project, we have worked in several areas relevant to low-dose ionizing radiation. Using gene expression to measure biological response, we have examined the response of human skin exposed in-vivo to radation, human skin exposed ex-vivo to radiation, and a human-skin model exposed to radiation. We have learned a great deal about the biological response of human skin to low-dose ionizing radiation.

Rocke, David M. [University of California Davis

2013-09-09T23:59:59.000Z

26

Low Dose Ionizing Radiation and HZE Particle Effects on Adult Hippocampal  

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and HZE Particle Effects on Adult Hippocampal and HZE Particle Effects on Adult Hippocampal Neurogenesis and mRNA Expression Kerry O'Banion University of Rochester School of Medicine & Dentistry Abstract Most of our knowledge about low dose radiation effects relates to DNA damage and chromosomal aberrations that result in cell death or alterations in genetic programs leading to malignancy. In addition To direct DNA damage, there is accumulating evidence that radiation induced alterations in the microenvironment can have significant effects on programs of cell replication and differentiation such as neurogenesis in adult mammalian brain. Adult neurogenesis in the hippocampus is postulated to play an important role in learning and memory and manipulations that alter neurogenesis, including inhibition following radiation exposure, have been

27

Low Dose Radiation Research Program: Molecular Characterization...  

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the Role of SOD Genes in Mammalian Cellular Response to Low Dose Ionizing Radiation Chaun-Yuan Li Duke University Medical Center Durham, NC Why this Project? To evaluate the roles...

28

Low Dose Radiation Research Program: Low Dose Radiation Research...  

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Low Dose Radiation Research: Outreach and Resources Authors: Antone L. Brooks and Lezlie A. Couch Institution: Washington State University Tri-Cities, Richland, Washington The...

29

Low Dose Radiation Program: Workshop VI Abstracts  

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Workshop VI Principal Investigator and Abstracts Workshop VI Principal Investigator and Abstracts Anderson, Carl Whole Genome Analysis of Functional Protein Binding Sites and DNA Methylation: Application to p53 and Low Dose Ionizing Radiation. Averbeck, Dietrich Cellular Responses at Low Doses of Ionizing Radiation. Azzam, Edouard Adaptive Responses to Low Dose/Low Dose-Rate ?-Rays in Normal Human Fibroblasts: The Role of Oxidative Metabolism. Bailey, Susan The Role of Telomere Dysfunction in Driving Genomic Instability. Balajee, Adayabalam Low Dose Radiation Induced DNA Damage Signaling and Repair Responses in Human 3-Dimensional Skin Model System. Barcellos-Hoff, Mary Helen Imaging Bioinformatics for Mapping Multidimensional Responses. Barcellos-Hoff, Mary Helen Biological Response to Radiation Mediated through the Microenvironment and

30

Low Dose Radiation Program: Links - Organizations Conducting Radiation  

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Conducting Low Dose Radiation Research Conducting Low Dose Radiation Research DOE Low Dose Radiation Research Program DoReMi Integrating Low Dose Research High Level Expert Group (HLEG) on European Low Dose Risk Research Multidisciplinary European Low Dose Initiative (MELODI) RISC-RAD Radiosensitivity of Individuals and Susceptibility to Cancer induced by Ionizing Radiation United States Transuranium & Uranium Registries Organizations Conducting other Radiation Research Argonne National Laboratory (ANL) Armed Forces Radiology Research Institute (AFRRI) Atmospheric Radiation Measurement (ARM) Program Brookhaven National Laboratory (BNL) Center for Devices and Radiological Health (CDRH) Central Research Institute of Electric Power Industry (CRIEPI) Colorado State University Columbia University

31

Low Dose Radiation Program: 2010 Low Dose Radiation Research Program  

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Low Dose Radiation Research Program Investigators' Workshop Low Dose Radiation Research Program Investigators' Workshop »» Event Slide Show More than 150 people attended this year's workshop, held April 12-14 at the Renaissance M Street Hotel in Washington, D.C. In addition to 34 plenary talks and more than 70 poster presentations made by the program investigators, participants heard guest speakers from the National Cancer Institute and from sister low-dose programs in Europe and Japan. Remarks from DOE Dr. Anna Palmisano, Associate Director, Office of Science, Director for Biological and Environmental Research (BER), welcomed the meeting participants, thanked Low Dose Radiation Research Program Manager Dr. Noelle Metting for her leadership, and acknowledged the importance of the Low Dose Program to DOE because of its unique focus and important role. She

32

Risk of Low Dose/Low Dose Rate Ionizing Radiation to Humans Symposium at the EMS 2009 Annual Meeting - September 2006  

Science Conference Proceedings (OSTI)

The low dose symposium thoughtfully addressed controversy of risk from low dose radiation exposure, hormesis and radon therapy. The stem cell symposium cogently considered the role of DNA damage and repair in hematopoietic stem cells underlying aging and malignancy and provocatively presented evidence that stem cells may have distinct morphologies and replicative properties, as well as special roles in cancer initiation. In the epigenetics symposium, studies illustrated the long range interaction of epigenetic mechanisms, the roles of CTCF and BORIS in region/specific regulation of epigenetic processes, the impact of DNA damage on epigenetic processes as well as links between epigenetic mechanisms and early nutrition and bystander effects.

Morgan, William F.; von Borstel, Robert C.; Brenner,; Redpath, J. Leslie; Erickson, Barbra E.; Brooks,

2009-11-12T23:59:59.000Z

33

Low-Dose Radiation Cataract and Genetic Determinants of Radiosensitivi...  

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Our research utilizes radiation cataract as a model system to study the effects of low-dose ionizing radiation exposure in a complex, highly differentiated tissue. We believe...

34

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary glandas a means of understanding human risk for breast cancer  

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susceptibility to low-dose ionizing radiation in the mouse mammary gland susceptibility to low-dose ionizing radiation in the mouse mammary gland as a means of understanding human risk for breast cancer Antoine M. Snijders 1 , Francesco Marchetti 1 , Ju Han 1 , Sandhya Bhatnagar 1 , Nadire Duru 1 , Zhi Hu 1 , Jian-Hua Mao 1 , Mina Bissell 1 , Joe Gray 1,2 , Gary H. Karpen 1 , Priscilla K. Cooper 1 and Andrew J. Wyrobek 1 1 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 2 Current affiliation: Biomedical Engineering, Oregon Health Science Univ, Portland, OR Goal: Our goal is to develop an in vivo mechanistic model of genetic variation in the low-dose damage responses of mammary glands using inbred mice known to vary in their sensitivity to low-dose induced mammary gland cancer, and to develop molecular predictors for susceptibility or resistance to low-dose induced breast cancer.

35

Review and Evaluation of Updated Research on the Health Effects Associated with Low-Dose Ionizing Radiation  

Science Conference Proceedings (OSTI)

Potential health effects of low levels of radiation have predominantly been based on those effects observed at high levels of radiation. The authors have reviewed more than 200 percent publications in radiobiology and epidermiology related to low dose radiation and concluded that recent radiobiological studies at low-doses; that doses low dose radiation research should to holistic, systems-based approaches to develop models that define the shape of the dose-response relationships at low doses; and that these results should be combined with the latest epidermiology to produce a comprehensive understanding of radiation effects that addresses both damage, likely with a linear effect, and response, possibly with non-linear consequences.

Dauer, Lawrence T.; Brooks, Antone L.; Hoel, David G.; Morgan, William F.; Stram, Daniel; Tran, Phung

2010-07-01T23:59:59.000Z

36

Low Dose Radiation Research Program: Proteomic and Biochemical...  

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proteomic changes including protein expression levels and post-translational modification due to low dose-rate ionizing radiation Expected Outcomes Increased differentiation...

37

Low Dose Radiation Research Program: 2011 Current Projects  

NLE Websites -- All DOE Office Websites (Extended Search)

Investigation of non-targeted effects of low dose ionizing radiation on the mammary gland utilizing three-dimensional culture models of mammary cells derived from mouse strains...

38

Radiation Leukaemongenesis at Low Doses  

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Leukaemongenesis at Low Doses Leukaemongenesis at Low Doses Simon Bouffler Health Protection Agency Abstract Myeloid leukaemias feature prominently among the cancers associated with human exposures to ionising radiation. The CBA mouse model of radiation-induced acute myeloid leukaemia (AML) has been used extensively for both quantitative and mechanistic studies. Loss of genetic material from chromosome 2 (chr2) is known to be associated with most radiation-induced AMLs. AML develops in CBA mice exposed to X- or γ-radiation, after a mean latency period of 18 months, with a maximal incidence of approximately 25% at 3Gy. A strong candidate AML-suppressor gene located within the commonly deleted region of chr2 has been identified, Sƒpil/PU.1. This gene suffers hemizygous loss and specific

39

Low Dose Radiation Research Program: Bruce E. Lehnert  

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E. Lehnert E. Lehnert Los Alamos National Laboratory Past Project Low Dose Ionizing Radiation-Induced Effects in Irradiated and Unirradiated Cells: Pathways Analysis in Support of Risk Assessment. Technical Abstracts 2002 Workshop: Low Dose Ionizing Radiation-Induced Effects in Irradiated and Unirradiated cells: Pathways Analysis in Support of Risk Assessment. Lehnert, B.E., Cary, R., Gadbois, D. and Gupta G. 2001 Workshop: Low Dose, Low Dose Rate Effects of Ionizing Radiation in Irradiated and Unirradiated Cells. Lehnert, B.E. 1999 Workshop: Low Dose, Low Dose Rate Effects of Ionizing Radiation in Irradiated and Unirradiated Cells. Lehnert, B.E. Publications Lehnert, B.E., Radiation bystander effects. U.S.Department of Energy Research News (March 6 issue) Goldberg, Z. and Lehnert, B.E. (2002). Radiation-induced effects in

40

Low Dose Radiation Research Program: Aloke Chatterjee  

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Chatterjee, A. and Holley, W.R. 1999 Workshop: Biological Effects of Low-Dose and Low-Dose-Rate Radiation Exposures: An Integrated Theoretical and Experimental Approach....

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Low Dose Radiation Research Program: Image Gallery  

NLE Websites -- All DOE Office Websites (Extended Search)

Image Gallery Image Gallery These are images, photographs, and charts presented or developed for Low Dose Radiation Research Investigators’ Meetings. They may be used for presentations or reports. To save, right click on the picture, then choose "Save picture as." U.S. annual per-capita effective radiation dose from various sources for 1980. various sources 1980 Enlarge Image. U.S. annual per-capita effective radiation dose from various sources for 2006. various sources 2006 Enlarge Image. U.S. annual per-capita effective radiation dose from man-made sources in the United States for 2006. man-made 2006 Enlarge Image. Ionizing Radiation Dose Ranges showing the wide range of radiation doses that humans experience (Rem) Enlarge Image. Ionizing Radiation Dose Ranges showing the wide range of radiation doses that humans experience

42

Low Dose Radiation Research Program: Slide Shows  

NLE Websites -- All DOE Office Websites (Extended Search)

Dose Health Effects of Radiation Health Effects of Radiation Adaptive Response to Low Dose Radiation PDF Background Radiation PDF Bystander Effects PDF Dirty Bombs PDF DNA Damage...

43

Low Dose Radiation Program: Links - Online Literature  

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Online Literature Online Literature Journals, Books and other Publications Armed Forces Radiobiology Research Institute Chornobyl Center for Nuclear Safety Radioactive Waste and Radioecology "Insight" Magazine Central Research Institute of the Electric Power Industry (CRIEPI) News: Aiming at an information center on low dose radiation research Health Physics International Journal of Radiation Biology Iranian Journal of Radiation Research Journal of Radiological Protection National Council on Radiation Protection and Measurements Radiation Research U.S. Department of Energy (DOE) Information Bridge Reports Animal Cancer Tests and Human Cancer Risk Assessment: A Broad Perspective Effects of Ionizing Radiation: Atomic Bomb Survivors and Their Children (1945-1995) Health Effects of Exposure to Low Levels of Ionizing Radiation: BEIR

44

Low Dose Radiation Program: Selected Websites  

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The views expressed in these links do not necessarily reflect the view of the Low Dose Radiation Research Program. Scientific Links Agencies with Radiation Regulatory...

45

Low Dose Radiation Research Program: Glossary  

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Glossary Glossary A B C D E F G H I J K L M N O P Q R S T U V W X Y Z We welcome updates to the glossary. Please send them to Low Dose. A α=β Ratio: A measure of the curvature of the cell survival curve and a measure of the sensitivity of a tissue or tumor to dose fractionation. The dose at which the linear and quadratic components of cell killing are equal. Abscopal Effect: The radiation response in tissue at a distance from the irradiated site invoked by local irradiation. Absorbed Dose Rate: Absorbed dose divided by the time it takes to deliver that dose. High dose rates are usually more damaging to humans and animals than low-dose rates. This is because repair of damage is more efficient when the dose rate is low. Absorbed Dose: The amount of energy deposited in any substance by ionizing

46

Low Dose Radiation Research Program: Mechanistic Modeling of...  

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Laboratory Why This Project? Cells that are directly exposed to low doses of ionizing radiation may experience DNA damage. Cells which happen to be in the vicinity of the exposed...

47

Low Dose Radiation Research Program: James D. Tucker  

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Role of the Adaptive Response in determining health risks from in vivo exposures to low dose of ionizing radiation Tucker, J. Publications Christian, A.T., Patte, M.S., Attix,...

48

Radiation Leukaemogenesis at Low Doses  

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myeloid leukaemia development at high and low doses. References 1. Cook WD, McCaw BJ, Herring C, John DL, Foote SJ, Nutt SL and Adams JM (2004). PU.1 is a suppressor of myeloid...

49

Low Dose Radiation Research Program: Katherine Vallis  

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Margaret Hospital Newly Funded Project The Characterization of Genetic Responses to Low Dose Radiation Using a Genome-Wide Insertional Mutagensis Approach Technical Abstracts 2005...

50

Low Dose Radiation Research Program: Mohan Natarajan  

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of Survival Advantage, Bystander Effect, and Genomic Instability after Low-LET Low Dose Radiation Exposure Funded Project Real-Time Molecular Study of Bystander Effect Using...

51

Low Dose Radiation Research Program: Multidimensional Analysis...  

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Multidimensional Analysis of Human Epithelial Cell Response to Low Dose Radiation Mary Helen Barcellos-Hoff Lawrence Berkeley National Laboratory Berkeley, CA. (Jointly funded by...

52

Low Dose Radiation Research Program: Genetic Factors Affecting  

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Affecting Susceptibility to Low-Dose Radiation Affecting Susceptibility to Low-Dose Radiation William F. Morgan Pacific Northwest National Laboratory Why This Project The short-term effects of high doses of ionizing radiation on cellular responses are relatively well understood. Less clear are the long-term consequences of exposure to low dose/low dose-rate radiation and the effects of radiation exposure on the progeny of surviving cells. If a cell survives radiation, it is generally thought to have repaired all the radiation-induced insults and be capable of a "normal healthy life". At a certain frequency, however, we have found that some cells surviving radiation grow normally, but will rearrange their genetic material during time in culture. We call this radiation-induced genomic instability. Many

53

Low Dose Radiation Research Program: National Laboratories  

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National Laboratories National Laboratories The Low Dose Radiation Program funding encompasses several Scientific Focus Areas (SFAs). The SFAs fund merit-reviewed research at DOE national laboratories. This management approach was created in 2008 by the Office of Biological and Environmental Research (BER) within the U.S. Department of Energy's (DOE's) Office of Science. PNNL's Low Dose Radiation Research Program Scientific Focus Area Linear and Nonlinear Tissue-Signaling Mechanisms in Response to Low Dose and Low Dose-Rate Radiation This program is funded as a U.S. Department of Energy Scientific Focus Area (SFA), and is an integrated cooperative program to understand low dose radiation effects in a complex model system. Coordinating Multidisciplinary Expertise The SFAs are designed to take advantage of the multidisciplinary,

54

Impact of Low-Dose Ionizing Irradiation on Histone Modification...  

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Impact of Low-Dose Ionizing Irradiation on Histone Modification and Chromatin Organization Hunter W. Richards Lawrence Berkeley National Laboratory Abstract Goal Our goal is to...

55

Impact of Low-Dose Ionizing Irradiation on Histone Modification...  

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Low-Dose Ionizing Irradiation on Histone Modification and Chromatin Organization Hunter W. Richards, Steven D. Ayers, Shutao Cai, Yoshinori Kohwi, Gary Karpen, Sylvain Costes and...

56

Low Dose Radiation Research Program: Genetic Factors Affecting  

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Genetic Factors Affecting Susceptibility to Low-Doses of Ionizing Genetic Factors Affecting Susceptibility to Low-Doses of Ionizing Radiation. Authors: William F. Morgan, Pat Concannon & John H.J. Petrini The goal of this program is to test the hypothesis that mice heterozygous for the NBS1 gene are genetically susceptible to low doses of ionizing radiation. Patients with Nijmegen Breakage Syndrome (NBS) are radiation sensitive, because of defects in cellular responses to radiation induced genetic damage. It is unclear whether humans heterozygous for the mutations associated with NBS are radiation sensitive and results from cell culture experiments give conflicting results. In collaboration with John Petrini at the Memorial Sloan Kettering Cancer Center in New York City we developed a mouse model of this disorder and are directly testing the hypothesis

57

Activation of nuclear factor kB in human lymphoblastoid cells by low-dose ionizing radiation  

SciTech Connect

Nuclear factor kB (NF-kB) is a pleiotropic transcription factor which is involved in the transcriptional regulation of several specific genes. Recent reports demonstrated that ionizing radiation in the dose range of 2-50 Gy results in expression of NF-kB in human KG-1 myeloid leukemia cells and human B-lymphocyte precursor cells; the precise mechanism involved and the significance are not yet known. The present report demonstrates that even lower doses of ionizing radiation, 0.25-2.0 Gy, are capable of inducing expression of NF-kB in EBV-transformed 244B human lymphoblastoid cells. These results are in a dose range where the viability of the cells remains very high. After exposure to {sup 137}Cs {gamma} rays at a dose rate of 1.17 Gy/min, a maximum in expression of NF-kB was seen at 8 h after a 0.5-Gy exposure. Time-course studies revealed a biphasic time-dependent expression after 0.5-, 1- and 2-Gy exposures. However, for each time examined, the expression of NF-kB was maximum after the 0.5-Gy exposure. The expression of the p50 and p65 NF-kB subunits was also shown to be regulated differentially after exposures to 1.0 and 2.0 Gy. 32 refs., 3 figs.

Prasad, A.V.; Mohan, N.; Meltz, M.L.; Chandrasekar, B. [Univ. of Texas Health Science Center, San Antonio, TX (United States)

1994-06-01T23:59:59.000Z

58

Low Dose Radiation Research Program: Universities  

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Universities Universities | Duke University | Loma Linda University | Northwestern University | University of Chicago | University of California Davis | Northwestern University University of Chicago University of California Davis Effects of Low Dose Irradiation on NF-κB Signaling Networks and Mitochondria Principal Investigator: Dr. Gayle Woloschak DOE Low Dose Research Program Projects Low dose-low dose rate irradiation leads to long term changes in numbers of mitochondria and mitochondrial genomes - Principal Investigator: Gayle Woloschak, Professor, Department of Radiation Oncology, Northwestern University, Chicago, IL, USA NF-κB-mediated pro-survival network in low dose radiation-induced adaptive protection - Principal Investigator: Jian Jian Li, Professor, Department of Radiation Oncology, University of California Davis, Davis,

59

Low Dose Radiation Research Program: DOE / NASA Joint Funded Projects  

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DOE/NASA Joint Funded Projects DOE/NASA Joint Funded Projects NASA Source Photo Space explorers are subject to exposure to low dose ionizing radiation. Research that helps determine health risks from this exposure is funded by NASA and DOE. Source: NASA DOE's Low Dose Program and the National Aeronautics and Space Administration (NASA) jointly fund new research to develop a better scientific basis for understanding risks to humans from exposures to low doses or low fluences of ionizing radiation. Research must focus on elucidating molecular mechanisms and pathways involved in normal radiobiological responses to low dose exposure, and must have the potential to ultimately increase understanding of health outcomes from radiation exposures that are at or near current workplace exposure

60

Low Dose Radiation Program: Links - Low Dose Research in Japan...  

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Low Dose Research in Japan-Institutes and Facilities Atomic Bomb Disease Institute, Nagasaki University Institute for Environmental Sciences (IES) National Institute of...

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We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Low Dose Radiation Program: Radiation Biology and the Radiation Research  

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Biology and the Radiation Research Program Biology and the Radiation Research Program The Department of Energy (DOE) and its predecessor organizations, Energy Research and Development Agency (ERDA) and Atomic Energy Commission (AEC), always have been concerned about the health effects of ionizing radiation. Extensive research has been conducted under their sponsorship at all levels of biological organization from molecules to man. Over the past 60 years, studies using every type of radiation source have included exposure to both external radiation sources and to internally deposited radioactive materials. These exposures used different dose patterns and distributions delivered over a wide range of experimental times. This extensive research provided the basis for the new Low Dose Radiation Research Program, linking

62

Cell Type-dependent Gene Transcription Profile in Three Dimensional Human Skin Tissue Model Exposed to Low Doses of Ionizing Radiation: Implications for Medical Exposures  

SciTech Connect

The concern over possible health risks from exposures to low doses of ionizing radiation has been driven largely by the increase in medical exposures, the routine implementation of X-ray backscatter devices for airport security screening, and, most recently, the nuclear incident in Japan. Due to a paucity of direct epidemiological data at very low doses, cancer risk must be estimated from high dose exposure scenarios. However, there is increasing evidence that low and high dose exposures result in different signaling events and may have different mechanisms of cancer induction. We have examined the radiation induced temporal response of an in vitro three dimensional (3D) human skin tissue model using microarray-based transcriptional profiling. Our data shows that exposure to 100 mGy of X-rays is sufficient to affect gene transcription. Cell type specific analysis showed significant changes in gene expression with the levels of > 1400 genes altered in the dermis and > 400 genes regulated in the epidermis. The two cell types rarely exhibited overlapping responses at the mRNA level. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) measurements validated the microarray data in both regulation direction and value. Key pathways identified relate to cell cycle regulation, immune responses, hypoxia, reactive oxygen signaling, and DNA damage repair. We discuss in particular the role of proliferation and emphasizing how the disregulation of cellular signaling in normal tissue may impact progression towards radiation induced secondary diseases.

Freiin von Neubeck, Claere H.; Shankaran, Harish; Karin, Norman J.; Kauer, Paula M.; Chrisler, William B.; Wang, Xihai; Robinson, Robert J.; Waters, Katrina M.; Tilton, Susan C.; Sowa, Marianne B.

2012-04-17T23:59:59.000Z

63

Low Dose Radiation Research Program: Low-Dose Dose-Response of  

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Low-Dose Dose-Response of Proliferating Human Cells Exposed to Low Low-Dose Dose-Response of Proliferating Human Cells Exposed to Low Dose Rate g-Radiation. Authors: Louise Enns,1 Michael Weinfeld,1 Albert Murtha,1 and Kenneth Bogen2 Institutions: 1Cross Cancer Institute and 2Lawrence Livermore National Laboratory. Clinical and environmental exposure to ionizing radiation rarely exceeds 200 cGy. To examine cell proliferation at early times (up to 5 days) post-irradiation, we are utilizing an assay in which single cells encapsulated within ~30- to 70-µm-diameter agarose gel microdrops (GMDs) are exposed and cultured for 4 days at 37°C, then analyzed by flow cytometry (FC). Clonogenic proliferation is measured as the fraction of occupied GMDs containing multicellular microcolonies after 4 days in culture. This assay was applied to human A549 lung cells exposed to gamma

64

Low Dose Radiation Research Program: Low Dose Radiation Effects in  

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Radiation Effects in Differentiating Human Lens Cells Radiation Effects in Differentiating Human Lens Cells E.A. Blakely1, M.P. McNamara1, P.Y. Chang1, K.A. Bjornstad1, D. Sudar1, and A.C. Thompson2 1Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California; 2Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, California. Introduction The human lens is one of the most radiosensitive organs of the body. Cataract, the opacification of the lens, is a late-appearing response to radiation damage. There are few data available on the late radiation effects of exposure in space flight to charged particle beams, the most prevalent of which are protons. Basic research in this area is needed to integrate the responses of both critical and other representative tissues

65

Low Dose Radiation Research Program: Thomas Weber  

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2005 Workshop: A Paracrine Signal Mediates The Cell Transformation Response To Low Dose Gamma Radiation in JB6 Cells. Weber, T.J., Siegel, R.W., Markillie, L.M., Chrisler,...

66

Low Dose Radiation Research Program: Micronutrient Deficiency...  

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DNA damage is appreciable and increases with age,3;4 Our aim is to compare low dose radiation with micronutrient deficiency, and endogenous damage by a variety of measures...

67

Low Dose Radiation Research Program: Chaun-Yuan Li  

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Chaun-Yuan Li Chaun-Yuan Li Radiation Biology Research, Duke University Medical Center Funded Projects Molecular Characterization of the Role of SOD Genes in Mammalian Cellular Response to Low Dose Ionizing, abstract, description. Technical Abstracts 2006 Workshop: The Roles of Superoxide Dismutage (SOD) in Low Dose Radiation Induced Adaptive Response Yang, Z., Chuang, E., Batinic-Haberle, I., and Li, C.-Y. 2005 Workshop: Molecular Characterization of the Roles of SOD Genes in Mammalian Cellular Response to Low Dose Radiation Li, C.-Y., Guo, Z., Yang, Z., and Chuang, E. 2003 Workshop: Molecular Characterization of the Roles of SOD Genes in Mammalian Cellular Response to Low Dose Radiation Li, C.-Y. and Chuang, E. Publications Li, F., Sonveaux, P., Rabbani, Z.N., Liu, S., Yan, B., Huang, Q.,

68

Low Dose Radiation Research Program: Current Funded Project Descriptions  

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Funded Project Descriptions Funded Project Descriptions Effects Of Low Doses of Radiation on DNA Repair Jointly funded by NASA and DOE Eric J Ackerman Pacific Northwest National Laboratory Richland, WA 99352 Dr. Ackerman will study the effect of low doses of ionizing radiation on the repair of different types of damage to DNA, including damage from ionizing radiation and that produced by the normal internal operation of the cell. Using a very sensitive technique called host cell reactivation assay (HCR), he will quantitatively measure the repair of each type of DNA damage and thereby measure if the cellular repair system itself has been damaged. He will also determine if unique forms of DNA repair system damage are induced by low doses of cosmic radiation exposure present during space

69

Low Dose Radiation Research Program: Project Descriptions-Archive  

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Project Descriptions-Archive Project Descriptions-Archive Effects Of Low Doses of Radiation on DNA Repair Eric J Ackerman (former PNNL) (Jointly funded by NASA and DOE) Pacific Northwest National Laboratory Richland, WA Dr. Ackerman will study the effect of low doses of ionizing radiation on the repair of different types of damage to DNA, including damage from ionizing radiation and that produced by the normal internal operation of the cell. Using a very sensitive technique called host cell reactivation assay (HCR), he will quantitatively measure the repair of each type of DNA damage and thereby measure if the cellular repair system itself has been damaged. He will also determine if unique forms of DNA repair system damage are induced by low doses of cosmic radiation exposure present during space

70

Low Dose Radiation Research Program: Research Institutions  

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Institutions Institutions Lovelace Respiratory Research Institute Biological Bases for Radiation Adaptive Responses in the Lung-Lovelace Respiratory Research Institute, Albuquerque, NM USA Contact: Dr. Bobby R. Scott Program Objective Our research focuses on elucidating the biological bases for radiation adaptive responses in the lung and for suppressing lung cancer, and to use the knowledge gained to produce an improved systems-biology-based, risk model for lung cancer induction by low-dose, low linear-energy-transfer (LET) radiation. Research was initiated in October 2009. This research should help foster a new era of low-dose radiation risk/benefit assessment. It will have important implications for possible use of low-dose diagnostic radiation (e.g., X-rays) in cancer therapy. It

71

Low Dose Radiation Research Program: Molecular Characterization of the  

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Molecular Characterization of the Roles of SOD Genes in Mammalian Molecular Characterization of the Roles of SOD Genes in Mammalian Cellular Response to Low Dose Radiation Authors: Chuan-Yuan Li, Zhanjun Guo, Zhonghui Yang, and Eric Chuang Institutions: Dept of Radiation Oncology, Duke University Medical Center, Durham, NC Advanced Technology Center, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland Background The potential risks of exposure to low dose radiation are of major concerns to the DOE/OBER Low Dose Radiation Research Program. It has been long recognized that much of the radiation-induced genetic damage to cells are caused by secondary oxidative species. Therefore internal cellular defense systems against oxidative stress play significant roles in countering genetic damage induced by ionizing radiation. The role of the detoxifying

72

Low Dose Radiation Research Program: 2003 Molecular Characterization of the  

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Characterization of the Roles of SOD Genes in Mammalian Characterization of the Roles of SOD Genes in Mammalian Cellular Response to Low Dose Radiation Authors: Chuan-Yuan Li,1 Eric Chuang2 Institutions: 1Dept of Radiation Oncology, Duke University Medical Center, Durham, NC, 2Advanced Technology Center, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland The potential risks of exposure to low dose radiation are of major concerns to the DOE/OBER Low Dose Radiation Research Program. It has been long recognized that much of the radiation-induced genetic damage to cells are caused by secondary oxidative species. Therefore, internal cellular defense systems against oxidative stress play significant roles in countering genetic damage induced by ionizing radiation. The role of the detoxifying

73

Health Risks Associated with Low Doses of Radiation  

Science Conference Proceedings (OSTI)

Despite a wealth of information, there remains uncertainty concerning human radiation effects at low dose levels. This report provides background information and a literature review of research on the potential health hazards associated with exposure to low-level ionizing radiation. Topics include radiation characteristics, protection standards, epidemiologic data and risk models, the nature of human health exposure-related effects, important radiation health studies to date, and the scientific method fo...

1994-09-17T23:59:59.000Z

74

Low dose diagnostic radiation exposure and cancer risk in Trp53...  

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University Abstract The cancer risk associated with exposure to low doses of ionizing radiation has traditionally been extrapolated from effects observed at high doses and high...

75

Radiation Leukemogenesis at Low Dose Rates  

SciTech Connect

The major goals of this program were to study the efficacy of low dose rate radiation exposures for the induction of acute myeloid leukemia (AML) and to characterize the leukemias that are caused by radiation exposures at low dose rate. An irradiator facility was designed and constructed that allows large numbers of mice to be irradiated at low dose rates for protracted periods (up to their life span). To the best of our knowledge this facility is unique in the US and it was subsequently used to study radioprotectors being developed for radiological defense (PLoS One. 7(3), e33044, 2012) and is currently being used to study the role of genetic background in susceptibility to radiation-induced lung cancer. One result of the irradiation was expected; low dose rate exposures are ineffective in inducing AML. However, another result was completely unexpected; the irradiated mice had a very high incidence of hepatocellular carcinoma (HCC), approximately 50%. It was unexpected because acute exposures are ineffective in increasing HCC incidence above background. This is a potential important finding for setting exposure limits because it supports the concept of an ?inverse dose rate effect? for some tumor types. That is, for the development of some tumor types low dose rate exposures carry greater risks than acute exposures.

Weil, Michael; Ullrich, Robert

2013-09-25T23:59:59.000Z

76

Low Dose Radiation Research Program: Induction of Genomic Instability in  

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Induction of Genomic Instability in vivo by Low Doses of 137Cs y Induction of Genomic Instability in vivo by Low Doses of 137Cs y rays, Authors: K. Rithidech1, E.B. Whorton2, M. Tungjai1, E. Ar-Bab1, S.R. Simon1, M. Tawde3 and C.W. Anderson3. Institutions: 1Pathology Department, Stony Brook University, NY 11794-8691, USA, 2University of Texas Medical Branch at Galveston, TX 77550-1047,3Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000. Information on potential health hazards of radiation at doses below or equal to the level traditionally requiring human radiation protection (less than or equal to 10 cGy) is currently lacking. It is therefore important to characterize early and subsequent in vivo biological response induced by low doses of ionizing radiation because such data should provide information that can help determine whether radiation at this dose level

77

Low Dose Radiation Program: Principal Program Contacts  

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to the Office of Biological and Environmental Research. Dr. Barcellos-Hoff's current research interests are studies of breast cancer and ionizing radiation. The goal of her...

78

Low Dose Radiation Research Program: Alec Moreley  

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Alec Moreley Technical Abstracts 1999 Workshop: Development of PCR-Based Methods for the Detection of Mutagenic Effects of Ionizing Radiation Morley, A., Turner, D., and Sykes, P....

79

Low Dose Radiation Research Program Website ?? Highlighting...  

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Website Highlighting Low Dose Research Bill Morgan Pacific Northwest National Laboratory Abstract The Low Dose Radiation Research Programs website is found at http:...

80

Low Dose Radiation Research Program: Genomic Instability and...  

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Genomic Instability and Low Dose Low Dose Rate Radiation. Authors: Lei Huang, Suzanne Grim, William F. Morgan Institutions: University of Maryland. Humans will always receive...

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Low Dose Radiation Research Program: Effects of Low Doses of Radiation on  

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Low Doses of Radiation on DNA Repair Low Doses of Radiation on DNA Repair Eric Ackerman Pacific Northwest National Laboratory Why this Project? Even low doses (0.1 Gy) exert measurable effects on DNA repair. The first-known oxidative lesion repaired only by nucleotide excision repair found in normal cells is cyclo-dA. This lesion is found in normal cells and thought to be a byproduct of oxidative metabolism. When this lesion occurs, it stimulates repair. If repair is stimulated by low dose radiation, there are some implications for human health. For example, do some individuals exhibit a greater, lower, or no stimulation to certain DNA lesions? If there are population polymorphism that influence DNA repair, then it would be possible to use our assay for screening individuals for repair sensitivity.

82

IMPRINTED GENES & TRANSPOSITIONS: EPIGENOMIC TARGETS FOR LOW DOSE RADIATION EFFECTS  

Science Conference Proceedings (OSTI)

The overall hypothesis of this grant application is that low dose ionizing radiation (LDIR) elicits adaptive responses in part by causing heritable DNA methylation changes in the epigenome. This novel postulate was tested by determining if the level of DNA methylation at the Agouti viable yellow (A{sup vy}) metastable locus is altered, in a dose-dependent manner, by low dose radiation exposure (radiation hormesis, bringing into question the assumption that every dose of radiation is harmful. Our findings not only have significant implications concerning the mechanism of hormesis, but they also emphasize the potential importance of this phenomenon in determining human risk at low radiation doses. Since the epigenetic regulation of genes varies markedly between species, the effect of LDIR on other epigenetically labile genes (e.g. imprinted genes) in animals and humans needs to be defined.

Randy Jirtle

2012-10-11T23:59:59.000Z

83

Low Dose Radiation Research Program: About  

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About About Background. Extensive research on the health effects of radiation using standard epidemiological and toxicological approaches has been done for decades to characterize responses of populations and individuals to high radiation doses, and to set exposure standards to protect both the public and the workforce. These standards were set using models that extrapolated from the cancers observed following exposure to high doses of radiation to predicted, but not measurable, changes in cancer frequency at low radiation doses. The use of models was necessary because of our inability to detect changes in cancer incidence following low doses of radiation. Historically, the predominant approach has been the Linear-no-Threshold model (see Wikipedia entry) and collective dose concept that assumes each unit of radiation, no

84

Low Dose Radiation Research Program: Adaptive Response of Mouse Skin  

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Adaptive Response of Mouse Skin Epithelial Cells to Low Dose Adaptive Response of Mouse Skin Epithelial Cells to Low Dose Ionizing Radiation: Induction of NF-κB, MnSOD, 14-3-3ζ and Cyclin B1 Authors: Jian Jian Li, Kazi M. Ahmed, Ming Fan, Shaozhong Dong, Douglas R. Spitz, and Cheng-Rong Yu Institutions: Division of Molecular Radiobiology, Purdue University School of Health Sciences, West Lafayette, Indiana; Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, Iowa; Molecular Immunology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland Gene expression profiles demonstrate that a group of key stress-responsive genes are associated with radiation exposure and may contribute to cellular

85

Chronic Low Dose Radiation Effects on Radiation Sensitivity  

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Chronic Low Dose Radiation Effects on Radiation Sensitivity Chronic Low Dose Radiation Effects on Radiation Sensitivity and Chromosome Instability Induction in TK6 Cells Schwartz J.L. 1 , Jordan R. 1 , Slovic J. 1 , Moruzzi A. 1 , Kimmel R. 2 , and Liber, H.L. 3 1 University of Washington, Seattle, WA; 2 Fred Hutchinson Cancer Research Center, Seattle, WA; 3 Colorado State University, Fort Collins, Colorado There are a number of cell responses that can be detected after low dose radiation exposures including the adaptive response, low dose hypersensitivity, and induced genomic instability. The relationship between these different phenomena is unknown. In this study, we measured adaptive responses, low dose hypersensitivity, and induced genomic instability in a human B-lymphoblastoid cell model, TK6, where we could genetically modify radiation responses by either over-expression of BCL-2 or deletion of TP53. TK6

86

Low Dose Radiation Exposure: Exploring Bystander Effects In Vivo.  

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Exposure: Exploring Bystander Effects Exposure: Exploring Bystander Effects In Vivo. 1 Blyth, B.J., 1 Sykes, P.J. 1 Department of Haematology and Genetic Pathology, Flinders University and Medical Centre, Bedford Park, South Australia, 5042, The general population is daily exposed to chronic, low doses of ionizing radiation from both natural and artificial sources. The shape of the radiation dose-response curve at these low doses is currently linearly extrapolated from data obtained after high dose exposure due to the low sensitivity of traditional biological assays after near-background exposures. At odds with this Linear No-Threshold model, are the phenomena collectively referred to as the radiation-induced bystander effect. The bystander effect describes a collection of in vitro

87

Persistent DNA damage foci, cellular senescence and low dose radiation  

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Persistent DNA damage foci, cellular senescence and low dose radiation Persistent DNA damage foci, cellular senescence and low dose radiation Denise Munoz 1 , Albert Davalos 1 , Francis Rodier 1 , Misako Kawahara 1 , Judith Campisi 1,2 and Steven Yannone 1,3 1 Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 84-171, Berkeley CA 94720; 2 Buck Institute for Age Research, 8001 Redwood Boulevard, Novato CA 94945; 3 Corresponding author Ionizing radiation (IR) induced DNA double-strand breaks (DSBs) are cytologically detectable as large nuclear foci that contain phosphorylated histone H2AX (γH2AX), the adaptor protein 53BP1, and several other proteins that participate in the sensing and processing of DNA damage (DNA damage foci). In normal human cells, moderately high IR (0.5-1 Gy) doses cause the rapid appearance of these foci (acute DNA damage foci), which gradually disappear

88

Low Dose Radiation Research Program: David J. Chen  

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2003 Workshop: Gene Expression Profile of Normal Human Fibroblast After Ionizing Irradiation, a comparison study between low dose and high dose. Chen, D.J. 2002 Workshop:...

89

Low Dose Radiation Research Program: Jian Jian Li  

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Jian Jian Li Jian Jian Li School of Health Sciences, Purdue University Newly Funded Projects Regulation of NF-kB and Mn SOD in Low Dose Radiation-Induced Adaptive Responses in Mouse and Human Skin Cells, abstract, description. Technical Abstracts 2006 Workshops: NF-kB Mediated Signaling Network in Low Dose X-Ray Induced Adaptive Protection on Mouse and Human Skin Epithelial Cells Ahmed, K.M., Fan, M., Spitz, and Li, J.J. 2005 Workshops: Adaptive Response of Mouse Skin Epithelial Cells to Low Dose Ionizing Radiation: Induction of NF-κB, MnSOD, 14-3-3ζ and Cyclin B1. Li, J.J., Ahmed, K.M., Fan, M., Dong, S., Spitz, D.R., and Yu, C.-R. 2003 Workshops: Gene Expression Profiles of Human Skin Keratinocytes Exposed to Acute and Chronic Ionizing Radiation Li, J.J., Ozeki, M., Wang, T., Tamae, D., Nelson, D., Wyrobek, A., and

90

Low Dose Radiation Research Program: Earlier Events  

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Earlier Events Earlier Events 2000 | 2001 | 2002 | 2003 | 2004 April 2000 Ionizing Radiation Science and Protection in the 21st Century, NCRP, April 5-6, Arlington, VA. RADIATION RESEARCH 2000, Association for Radiation Research, April 10-12, Bristol, UK. Florida Chapter of the Health Physics Society Spring 2000 Meeting, Gainesville, FL, April 13-14. 47th Annual Meeting of the Radiation Research Society, April 29-May 3, Albuquerque, NM. May 2000 IRPA-10 International Congress 2000, May 14-19, Hiroshima, Japan. IRPA-10 Secretariat, c/o Japan Convention Services, Inc., Nippon Press Center Building, 2-2-1, Uchisaiwai-cho, Chiyoda-ku, Tokyo 100, Japan. Phone: 81-3-3508-1214. Fax: 81-3-3508-0820. irpa10@convention.jp. 4th International Non-Ionizing Radiation Workshop, May 22-25, Kyoto,

91

Low Dose Radiation Research Program: Computational Modeling of Biochemical  

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Computational Modeling of Biochemical Pathways Linking Ionizing Computational Modeling of Biochemical Pathways Linking Ionizing Radiation to Cell Cycle Arrest, Apoptosis, and Tumor Incidence Authors: Yuchao Maggie Zhao and Rory Conolly Institutions: Center for Computational Systems Biology CIIT Centers for Health Research Long-Range Goal: To develop an integrated, computational framework for the prediction of low-dose-response to ionizing radiation (IR) in people. Methodology: To provide a flexible framework to evaluate mechanisms of cellular adaptive responses after exposure to IR, three progressively more complicated descriptions of biochemical pathways linking DNA damage with cell-cycle checkpoint control and apoptosis were developed. These descriptions focus on p53-dependent checkpoint arrest and apoptosis, p73-dependent apoptosis, and Chk2-dependent checkpoint arrest,

92

Molecular mechanisms and cellular consequences of low-dose exposure to ionizing  

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mechanisms and cellular consequences of low-dose exposure to ionizing mechanisms and cellular consequences of low-dose exposure to ionizing radiation Andrew J. Wyrobek 1 , Francesco Marchetti 1 , Xiu Lowe 1 , Xiaochen Lu 2 , Terumi Kohwi- Shigematsu 1 , Brian Davy 1 , Thomas E. Schmid 1 , Sylvia Ahn 1 , Tarlochan Nijjar 1 Matthew A. Coleman 2 , Contact information: ajwyrobek@gmail.com 1 Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 2 BioSciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA. The objectives of this research are to characterize the genome-wide molecular responses to low-dose ionizing radiation (<10cGy), to identify tissue and cell-type specific differences in pathways responses, and to identify the pivotal molecular pathway responses that control risks to genome integrity and health. This project utilizes mouse in

93

Low Dose Radiation Research Program: William F. Morgan  

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William F. Morgan William F. Morgan Pacific Northwest National Laboratory PO Box 999 Richland, Washington About this Project Projects Using a Low LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation. Optimizing the Scientific, Regulatory, and Societal Impact of the DOE Low Dose Radiation Research Program A Mechanistic Study of the Radiation Quality Dependence of Bystander Effects in Human Cells. Genetic Factors Affecting Susceptibility to Low-Dose Radiation Mechanisms of Adaptive Responses and Genomic Instability Induced by Low Dose/ Low Dose Rate Radiation Technical Abstracts 2006 Workshop: Using a Low-LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation Sowa, M.B., Goetz, W., Baulch, J., and Morgan, W.F. Genetic Factors Affecting Susceptibility to Low-Dose Radiation

94

Analysis of low dose radiation induced epigenetic modifications...  

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levels ofbiological organization when organisms are exposed to low doses (<0.1Gy) of irradiation.Recent work in determining the exact effects of low dose radiation have shown that...

95

Low Dose Radiation Research Program: Cytogenetic tests of Radiobiologi...  

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relevant low-dose range (less than 0.1 Gy). Relate chromosome damage to radiation-induced cancer. Research Approach By studying molecular mechanisms relevant to low doses and low...

96

Low Dose Radiation Research Program: Kenneth T. Bogen  

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T. Bogen Lawrence Livermore National Laboratory Technical Abstracts 2002 Workshop: Low-dose dose-response of proliferating human cells exposed to low dose rate g-radiation. Enns,...

97

Low Dose Radiation Research Program: Transgenerational Effects...  

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Transgenerational Effects of Chronic Low-Dose Irradiation in a Medaka Fish Model System Colorado State University Why this Project? There are major gaps in our knowledge about...

98

Low Dose Radiation Research Program: Research Highlights  

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energy, are the surrounding unirradiated cells also at an increased risk of cancer? Latest Research, Response to Fukushima, Integrating Low Dose into Policy-All Featured at...

99

Low Dose Radiation Research Program: Effects of Low Doses of Radiation on  

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Abstract Abstract Title: Effects of Low Doses of Radiation on DNA Repair (PNNL Project # 42699) Authors: Eric J. Ackerman, Ph.D. Institutions: Pacific Northwest National Laboratory Richland, WA We developed a functional assay to measure the effects of LDR on repair of many different lesions representative of those found in cells as consequences of normal oxidative metabolism, as well as those caused by radiation. Currently only 1/10th attomole =105 damaged molecules/cell and 3000 cells/measurement are required. We have found that even low doses (10 rad) exert measurable effects on DNA repair. Interestingly, the amount of DNA repair increases at 10-50 rads, plateaus, and then increases even further at higher doses well below doses where radiation-induced lethality

100

Low dose radiation combines with the Src oncoprotein to transform  

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radiation combines with the Src oncoprotein to transform radiation combines with the Src oncoprotein to transform pre-malignant human breast cells Paul Yaswen Lawrence Berkeley National Laboratory Abstract Goal: Determine whether low dose radiation exerts persistent epigenetic effects that promote malignancy. Background and Significance: Some persistent carcinogenic effects of radiation may not be traceable to specific DNA sequence alterations and may not be linearly related to dose. Through the biochemical initiation of positive feedback loops, ionization-induced events may have heritable non-linear effects on cellular behavior. Inflammatory responses involving the transcription factor NFκB may be subject to such effects. Increased NFκB activity has been strongly linked to carcinogenesis in a number of published in vitro and in vivo studies (reviewed in [1]). Since radiation

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101

Low Dose Radiation Research Program: Low Dose Response of Respiratory Cells  

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Low Dose Radiation Research Program: Low Dose Response of Respiratory Low Dose Radiation Research Program: Low Dose Response of Respiratory Cells in Intact Tissues and Reconstituted Tissue Constructs Authors: John Ford, Amy Maslowski, Alex Redd and Les Braby Institutions: Texas A&M University, College Station, TX We are developing a model of respiratory tissue using a perfusion culture system. We are using this system to quantify the effects of normal tissue architecture, and the interaction of epithelial cells with other cell types, on radiation-induced bystander effects. Tracheal tissue taken from young adult Fischer 344 rats is imbedded in a growth factor enriched agarose matrix. The chamber is designed to allow growth medium to periodically wash the epithelial surface of the tracheal lumen while maintaining the air-interface that is necessary for the normal

102

Low Dose Radiation Research Program: Research Program Workshop I Abstracts  

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Genetic Factors Affecting Susceptibility to Low-Dose Radiation Genetic Factors Affecting Susceptibility to Low-Dose Radiation William F. Morgan and John H.J. Petrini Radiation Oncology Research Laboratory, University of Maryland at Baltimore, Baltimore, MD Summary: The goal of our application is to improve the scientific basis for understanding potential risks to the population from low dose radiation exposure based on potential genetic differences that may modulate an individual's sensitivity to low doses of radiation. Abstract: The goal of this application is to improve the scientific basis for understanding potential risks to the population from low dose radiation exposure. We propose to address specific genetic factors that affect individual susceptibility to low dose radiation and ask the question do genetic differences exist that make some individuals more sensitive to

103

Low Dose Radiation Research Program: Original Research Program Plan  

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Original Research Program Plan Original Research Program Plan Biological Effects of Low Dose and Dose Rate Radiation Prepared for the Office of Biological and Environmental Research by The Low Dose Radiation Research Program Plan Subcommittee of the Biological and Environmental Research Advisory Committee. II. Table of Contents Face Page Table of Contents Executive Summary Introduction Program Outline Low Dose Radiation vs. Endogenous Oxidative Damage - The Same or Different? Key Question Description Decision Making Value Recommendations and Costs Understanding Biological Responses to Radiation And Endogenous Damage Key Question Description Decision Making Value Recommendations and Costs Thresholds for Low Dose Radiation - Fact or Fiction? Key Question Description Decision Making Value Recommendations and Costs

104

Low Dose Radiation Research Program: Michael Weil  

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Dubrova, Y., Weil, M., and Brenner, D. H2AX Foci after Low Dose-Rate Irradiation Reveal a Defect in DNA DSB Processing in Cells from Unaffected Parents of...

105

Low Dose Radiation Research Program: Mina Bissell  

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Abstracts 2006 Workshop: 3D Tissue Models for the Study of the Effects of Low-Dose Irradiation Nelson, C.M., Fata, J E., Kenny, P.A., and Bissell, M.J. Publications Kumari, I.,...

106

Low Dose Radiation Research Program: David Nelson  

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L.E., Nelson, D., Sorensen, K., Tucker, J.D., and Wyrobek, A.J. (2005). Low-dose irradiation alters the transcript profiles of human lymphoblasoid cells, including genes...

107

Low Dose Radiation Research Program: Assessing Biological Function...  

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Livermore National Laboratory under contract No. W-7405-ENG-48 and funded by the Low Dose Radiation Research Program, Biological and Environmental Research (BER), U.S....

108

Low Dose Radiation Research Program: Genetic Variation in Tissue...  

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Variation in Tissue Responses to Low-dose Radiation Eugene Rinchik Oak Ridge National Laboratory Why this Project? To address how individual genetic background affects tissue...

109

Low dose radiation combines with the Src oncoproteinto transform...  

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Lawrence Berkeley National Laboratory, Berkeley CA 94720 Goal: Determine whether low dose radiation exerts persistent epigenetic effects that promote malignancy. Background and...

110

Low Dose Radiation Research Program: Bruce N. Ames  

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University of California, Berkeley Technical Abstracts 2002 Workshop: Comparison of Low-Dose Radiation, Endogenous Oxidants, and Micronutrient Deficiencies through Analyses of DNA...

111

Low Dose Radiation Research Program: Gene Expression Profiles...  

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of Energy by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48 with funding from the DOE Low Dose Radiation Research Program...

112

Low Dose Radiation Research Program: Susan S. Wallace  

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Susan S. Wallace University of Vermont Past Funded Project Free Radical DNA Damage Produced Endogenously and by Low Dose Radiation in Human Cells: Quantitation, Consequences, and...

113

Program on Technology Innovation: Evaluation of Updated Research on the Health Effects and Risks Associated with Low-Dose Ionizing Radiation  

Science Conference Proceedings (OSTI)

The Electric Power Research Institute (EPRI) has performed a systematic review of recently published, peer-reviewed scientific studies in the fields of epidemiology and radiobiology that discuss health risks associated with exposure to low levels of ionizing radiation. As a result of this study, the EPRI team concludes that there is a need to re-evaluate the magnitude of dose and dose-rate effectiveness factors (DDREF), including the significant body of radiobiology data that suggests non-linear risks at...

2009-11-18T23:59:59.000Z

114

Low Dose Radiation Research Program: Cooperation Between Homologous  

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Cooperation Between Homologous Recombination and the Fanconi Anemia Cooperation Between Homologous Recombination and the Fanconi Anemia Cancer Suppressor Proteins in Minimizing Spontaneous and Radiation-Induced Chromosomal Instability Authors: Larry H. Thompson, John M. Hinz, Robert S. Tebbs, and N. Alice Yamada Institutions: Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, California Purpose and experimental approach. This study addresses the genetic basis of spontaneous mutagenesis as a means of understanding the DNA damage-response pathways that maintain chromosome stability. It is our view that knowledge of these processes is fundamental to understanding how low dose ionizing radiation (IR) produces chromosomal rearrangements that lead to carcinogenesis. Endogenous oxidative DNA damage is presumed to be a

115

Low Dose Radiation Research Program: Linking Molecular Events to Cellular  

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Linking Molecular Events to Cellular Responses at Low Dose Exposures Linking Molecular Events to Cellular Responses at Low Dose Exposures Thomas Weber Pacific Northwest National Laboratory Why This Project It currently costs billions of dollars to protect workers and the public from exposure to man-made radiation, despite exposure levels lower than the natural background levels of radiation. If it could be demonstrated that there is no increased cancer risk associated with these low dose exposures, these resources could be directed toward more critical societal issues. Defining low dose radiation cancer risks is limited by our ability to measure and directly correlate relevant cellular and molecular responses occurring at the low radiation dose and dose rate with tumor formation. This deficiency has led to conservative risk assessments based on low dose

116

Low Dose Radiation Research Program: DOE Lowdose Radiation Program Workshop  

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Using a Low LET Electron Microbeam to Investigate Non-Targeted Using a Low LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation. Authors: William F. Morgan1 and Marianne B. Sowa2 Institutions: 1Radiation Oncology Research Laboratory, University of Maryland, Baltimore MD 21201 2 Chemical Structure and Dynamics, Pacific Northwest National Laboratory, Richland WA 99352 We have recently installed a low LET electron microbeam that generates energetic electrons to mimic radiation damage from gamma and x-ray sources. It has been designed such that high-energy electrons deposit energy in a pre-selected subset of cells leaving neighboring cells unirradiated (Figure 1). In this way it is possible to examine non-targeted effects associated with low dose radiation exposure including induced genomic instability and

117

Low Dose Radiation Research Program: Assessing Biological Function of DNA  

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Assessing Biological Function of DNA Damage Response Genes Assessing Biological Function of DNA Damage Response Genes Larry H. Thompson Lawrence Livermore National Laboratory Why This Project To understand the relative importance of individual DNA repair and DNA-damage response pathways to the recovery of mammalian cells after exposure to low doses of ionizing radiation (IR). This understanding may lead to better ways of setting limits on human exposure to IR. In spite of the discovery of many mammalian DNA repair genes, our current knowledge of how many of these genes contribute to cellular recovery from IR exposure is quite limited. Project Goals Measure cellular responses at doses in the 5-100 cGy range, which generally cause changes too small to detect in normal, repair-proficient cells Focus on DNA double-strand breaks (DSBs) and DNA oxidative base

118

Molecular Mechanism Underlying Cellular Adaptive Response to Low Dose Radiation  

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Mechanism Underlying Cellular Adaptive Response to Low Dose Radiation Mechanism Underlying Cellular Adaptive Response to Low Dose Radiation Colette A. Sacksteder § , DJ Black ‡ , Heather Smallwood § , David G. Camp II † , and Thomas C. Squier § § Cell Biology and Biochemistry; † Biological Sciences Division Pacific Northwest National Laboratory, Richland WA 99352 ‡ School of Biological Sciences, University of Missouri, Kansas City MO 64110 The goal of this research is to identify the molecular mechanisms by which cells adapt to low dose radiation exposure. Previously we have shown a radiation dependent increase of Calmodulin (CaM) in RAW 264.7 macrophages (RAW). Therefore we hypothesize that CaM and associated signaling complexes are sensors of low-dose radiation, resulting in alterations in energy metabolism and gene expression. The ultimate experimental goal

119

Low Dose Radiation Research Program: Antone (Tony) L. Brooks  

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A.L. and Couch, L.A. 2002 Workshop: Optimizing the Scientific, Regulatory and Social Impact of the DOE Low Dose Radiation Research Program Brooks, A.L., Bull, R.J., and...

120

Low Dose Radiation Research Program: John S. Wassom  

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the Science of the Low Dose Radiation Research Program. Wassom, J.S., Owens, E.T., Martin, S.A., Wolfe, A.K., Lyday, M.K., and Dimmick, S.L. 2001 Workshop: Communicating the...

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121

Low Dose Radiation Research Program: Research Highlights - Health Physics  

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Health Physics Special Issue Features Contributions by Low Dose Health Physics Special Issue Features Contributions by Low Dose Investigators Health Physics The March 2011 special issue of Health Physics highlights the Victor Bond Workshop held May 2-5, 2010, in Richland, Wash. The workshop honored the late Dr. Victor (Vic) Bond for his lifetime achievement in the radiation sciences. Dr. Bond's research resulted in numerous influential scientific papers that contributed greatly to the understanding of radiation effects in biological systems. The workshop attracted internationally recognized experts in biophysics, experimental radiation biology, epidemiology, and risk assessment to discuss issues of low-dose risk. Participants included current and previously funded U.S. Department of Energy Low Dose Radiation Research

122

Low Dose Radiation Research Program: Suresh H. Moolgavkar  

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cohort with low-LET low-dose radiation exposure, and comparison with Japanese atomic bomb survivors. Hazelton, W.D., Krewski, D., Moolgavkar, S.H. 2001 Workshop:...

123

Low Dose Radiation Research Program: Genetic Variation in Tissue...  

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Genetic Variation in Tissue Responses to Low-Dose Radiation Authors: E. M. Rinchik,1,2 P. Hoyt,3 L. Branstetter,1 R. Olszewski,1 K. T. Cain,1 and B. Voy1,3 Institutions: 1Life...

124

Low Dose Radiation Research Program: The Characterization of...  

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The Characterization of Genetic Responses to Low Dose Radiation using a Genome-Wide Insertional Mutagenesis Approach Authors: Katherine A Vallis,1,2,3 William L Stanford,4 Zhuo...

125

Low Dose Radiation Research Program: Comparison of DNA Damage...  

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Comparison of DNA Damage Risk from Low-Dose Radiation and Folate Deficiency Arnold C. Huang,1,2 Chantal Courtemanche,1,2 Nicole Kerry,1,2 Susan T. Mashiyama,1,2 Michael Fenech,3...

126

Low Dose Radiation Research Program: 2011 Calendar of Upcoming...  

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Ann. Mtg. of the American Roentgen Ray Society, Chicago, IL, USA. May 9-11, 2011 Low Dose Radiation Research Investigators' Workshop, Bethesda, MD, USA. May 16-20, 2011...

127

Low Dose Radiation-Induced Epigenetic Alterations Found in Agouti...  

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Low Dose Radiation-Induced Epigenetic Alterations Found in Agouti Mouse Model Autumn Bernal Autumn Bernal Randy Jirtle Randy Jirtle In a paper published in The FASEB Journal, Low...

128

Co-Regulation among genes and pathways that are responsive to low-dose ionizing  

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Regulation among genes and pathways that are responsive to low-dose ionizing Regulation among genes and pathways that are responsive to low-dose ionizing radiation. Matthew A. Coleman 1 , Anya Krefft 1 , Francesca Pearson 1 , Leif E. Peterson 2 , Jian Jian Li 3 , Xiaowen Xin 1 , Terrence Critchlow 1 , Ilkay Altintas 4 , Bertram Ludaescher 5 and Andrew J. Wyrobek 6 . 1 Biosciences, LLNL, Livermore, CA, 94550, 2 Departments of Molecular and Human Genetics and Medicine, Baylor College of Medicine, Houston, TX, 77030, 3 School of Health Sciences, Purdue University, West Lafayette, IN, 47907. 4 San Diego Supercomputer Center, University of California, San Diego La Jolla, CA 92093. 5 Department of Computer Science, University of California, Davis, CA 95616. 6 Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA. 94706. Contact information: coleman16@llnl.gov

129

Low Dose Radiation Research Program Publications  

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for medical exposures. Environmental and Molecular Mutagenesis 53(4): 247259 Lynn Hlatky ( 2012) Double-Strand Break Motions Shift Radiation Risk Notions?. PNAS...

130

Low Dose Radiation Research Program: Charles Limoli  

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Radiation Biology, University of California, Irvine Funded Project Radiobiology of Neural Cancer Stem Cells Publications Elmore, E., Lao, X.Y., Kapadia, R., Giedzinski, E., Limoli,...

131

Low Dose Radiation Research Program: Characterizing Bystander...  

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To order to investigate these variables for low-linear transfer (LET) radiation, an electron microbeam irradiation system has been developed. An electron source provides a beam...

132

Low Dose Radiation Program: Workshops, Proceedings, Abstracts and Programs  

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Workshops, Proceedings, Abstracts and Programs Workshops, Proceedings, Abstracts and Programs UPDATE: Investigators' Workshop Postponed The annual Low Dose Radiation Research Program Investigators' Workshop typically held in April or May has been postponed until next year. Please keep checking the website for updates. 2010 The 2010 DOE Low Dose Radiation Research Investigators' Workshop was held April 12-14, 2010, at the Renaissance M Street Hotel in Washington, D.C. In addition to 34 plenary talks and more than 70 poster presentations made by the program investigators, participants heard guest speakers from the National Cancer Institute and from sister low-dose programs in Europe and Japan. See link for investigators' abstracts. 2009 The DOE Low Dose Radiation Research Investigators' Workshop VIII was held

133

Global methylation responses to low dose radiation exposure  

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methylation responses to low dose radiation exposure methylation responses to low dose radiation exposure Pamela J Sykes, Michelle R Newman, Benjamin J Blyth and Rebecca J Ormsby Haematology and Genetic Pathology, Flinders University and Medical Centre, Flinders Centre for Cancer Prevention and Control, Bedford Park, Adelaide, South Australia 5042 Australia. (pam.sykes@flinders.edu.au). Our goal is to study the mechanisms involved in biological responses to low doses of radiation in vivo in the dose range that is relevant to population and occupational exposures. At high radiation doses, DNA double-strand breaks are considered the critical lesion underlying the initiation of radiation-induced carcinogenesis. However, at the very low radiation doses relevant for the general public, the induction of DNA double-strand breaks

134

Low Dose Radiation Research Program: Melvyn Folkard  

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Melvyn Folkard Melvyn Folkard Gray Cancer Institute About this Project Currently Funded Projects A Variable Energy Soft X-ray Microprobe to Investigate Mechanisms of the Radiation-Induced Bystander Effect Technical Abstracts 2005 Workshop: A Variable-Energy Soft X-Ray Microprobe to Investiage Mechanisms of the Radiation-Induced Bystander Effect Folkard, M., Vojnovic, B., Schettiono, G., Atkinson, K., Prise, K.M., Michael, B.D. 2003 Workshop: A Variable-Energy Soft X-Ray Microprobe to Investigate Mechanisms of the Radiation -Induced Bystander Effect. Folkard, M., Vojnovic, B., Schettino, G., Atkinson, K., Prise, K.M., Michael, B.D. 2002 Workshop: A Variable-Energy Soft X-ray Microprobe to Investigate Mechanisms of the Radiation-Induced Bystander Effect. Folkard, M., Vojnovic, B., Schettino,

135

Apoptosis as a Mechanism for Low Dose Radiation-and Amifostine-Mediated  

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Apoptosis as a Mechanism for Low Dose Radiation- and Amifostine-Mediated Apoptosis as a Mechanism for Low Dose Radiation- and Amifostine-Mediated Chromosomal Inversion Responses Pam Sykes Flinders University and Medical Centre Abstract Low dose radiation and the chemical radioprotector amifostine have both been shown to protect cells from the immediate and delayed effects of radiation exposure. They display a number of distinct similarities including their ability to protect cells against radiation-induced DNA damage, radiation-induced cell death and metastases formation. Amifostine, which protects cells from the toxic effects of ionizing radiation, has a broad range of activities including free radical scavenging, polyamine-like DNA binding, and induction of hypoxia and redox-regulated genes. Amifostine’s ability to protect cells is often

136

Low Dose Radiation Research Program: Radiation-Induced Nuclear Factor kB  

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Radiation-Induced Nuclear Factor kB mediates survival advantage by Radiation-Induced Nuclear Factor kB mediates survival advantage by Telomerase Activation. Authors: Natarajan M.,1 Mohan S.,2 Pandeswara, S.L.,1 and Herman T.S.1 Institutions: Departments of 1Radiation Oncology and 2Pathology, The University of Texas Health Science Center, San Antonio, Texas Activation of NF-kB in response to low doses of ionizing radiation was first shown in our laboratory. Although studies have shown that NF-kB plays an important role in anti-apoptotic function, little has been done to understand the molecular link between the activation of NF-kB and cellular outcome such as enhanced cell survival after low dose low-linear transfer (LET) radiation. Because upregulation of telomerase activity is associated with longevity and allows cells to escape from senescence, we hypothesize

137

Low Dose Radiation Research Program: Marianne B. Sowa  

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Marianne B. Sowa Marianne B. Sowa PNNL - Pacific Northwest National Laboratory Funded Projects A Mechanistic Study of the Radiation Quality Dependence of Bystander Effects in Human Cells Technical Abstracts 2006 Workshop: Using a Low-LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation. Sowa, M.B., Goetz, W., Baulch, J., and Morgan, W.F. Morphological Changes in a 3D Mammary Model Following Exposure to Low Dose, Low-LET Radiation Opresko, L.K., Chrisler, W., Emory, K., Arthurs, B., and Sowa, M.B. 2005 Workshops: Using a Low LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation Morgan, W.F. and Sowa, M.B. Publications Perrine, K.A., Lamarche, B.L., Hopkins, D.F., Budge, S.E., Opresko, L.K., Wiley, H.S., and Sowa, M.B. (2007). High speed method for in situ

138

Ionizing Radiation  

Science Conference Proceedings (OSTI)

*. Bookmark and Share. Ionizing Radiation Measurements. Fees for services are located directly below the technical contacts ...

2013-04-09T23:59:59.000Z

139

Low Dose Radiation Research Program: Do Heritable Differences in an  

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Do Heritable Differences in an Individual's Immune System Predict Do Heritable Differences in an Individual's Immune System Predict Differential Sensitivity to Low Dose Radiation Exposure? Quantitative trait loci (QTL) mapping Enlarge Image Quantitative trait loci (QTL) mapping identifies two strong candidate genes, Ptprk (Chr 10) and Acp1 (Chr 12) that are linked to genetic variation in the relative abundance of peripheral Th and Tc cells, two cell populations that are sensitive to radiation exposure at low doses. Expression of each gene is significantly altered by exposure to low dose radiation in vivo. Genome-wide scans for the ratio of %CD4+ (Th) to %CD8+ (Tc) lymphocytes were performed using genenetwork.org. The solid horizontal line represents genome-wide significance at P < 0.05, based on 1,000 permutations. LOD indicates logarithm of odds scores.

140

Low Dose Radiation Research Program: Using a Low LET Electron Microbeam to  

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a Low LET Electron Microbeam to Investigate Non-Targeted Effects of a Low LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation William F. Morgan Radiation Oncology Research Laboratory, Pacific Northwest National Laboratory Why this Project? To examine genomic instability and bystander effects as non-targeted effects associated with low dose radiation exposure. Project Goals To provides a robust, reliable, highly sensitive assay for detecting delayed events occurring in cells exposed to low doses of ionizing radiation. Experimental Approach To mimic radiation damage from gamma and x-ray sources, a low-LET electron microbeam that generates energetic electrons has been designed such that high-energy electrons deposit energy in a pre-selected subset of cells leaving neighboring cells unirradiated. Using a novel green fluorescence gene (GFP) reporter assay, a high through

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Low dose diagnostic radiation exposure and cancer risk in Trp53+/- mice  

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diagnostic radiation exposure and cancer risk in Trp53+/- mice diagnostic radiation exposure and cancer risk in Trp53+/- mice K Taylor, N Phan, ME Cybulski, L Laframboise, DR Boreham Department of Medical Physics and Applied Radiation Sciences, McMaster University, 1280 Main Street West, Hamilton ON L8S 4K1 The cancer risk associated with exposure to low doses of ionizing radiation has traditionally been extrapolated from effects observed at high doses and high dose rates using a linear no threshold model. Based on this approach, it has been postulated that human exposure to medical imaging involving low doses of x-rays and gamma rays increase an individual's risk of developing cancer throughout their lifetime. Conversely, there is evidence that low doses of gamma radiation increase the latency period of cancer depending upon genotype, cancer type, and the magnitude of

142

Low Dose Radiation Research Program: Low Dose Response of Respiratory Cells  

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Response of Respiratory Cells in Intact Tissues and Reconstituted Response of Respiratory Cells in Intact Tissues and Reconstituted Tissue John Ford Department of Nuclear Engineering Texas A & M University Why this Project? Using the well-established rat trachea model to test the hypothesis that normal respiratory epithelial cells transmit signals to neighboring cells in response to very low dose radiation exposure. Project Goals By comparing the responses shown by cells in these normal rodent respiratory tissues to those seen for human respiratory epithelial cells in reconstituted tissue constructs, it will be possible to better understand the responds in human respiratory cells in vivo. These studies will characterize responses after exposure to a variety of radiation types and dose distributions. Experimental Approach

143

Low Dose Radiation Research Program: Impact of Genetic Factors on the  

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Genetic Factors on the Heritable Effects of Paternal Exposure to Genetic Factors on the Heritable Effects of Paternal Exposure to Low-Dose Radiation Janet E. Baulch University of California, Davis Why This Project? There is concern about the possible genetic effects of low dose radiation exposure. As a result, much effort has gone towards understanding mutation of cells due to radiation exposure. While recognition of the potential for mutation from exposure to ionizing radiation has led to extensive research, less effort has been given to the possible delayed risk of radiation exposure transmitted to the offspring of the exposed parent. Data from animal models show that parental exposures to DNA-damaging agents, such as ionizing radiation, predispose the offspring to serious health effects, including cancer offspring. Additionally, data from both humans and animal

144

Low Dose Radiation Research Program: Optimizing the Scientific, Regulatory,  

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Optimizing the Scientific, Regulatory, and Societal Impact of the DOE Low Optimizing the Scientific, Regulatory, and Societal Impact of the DOE Low Dose Radiation Research Program Antone L. Brooks Why This Project? For maximum benefit, state-of-the-art research and new data from the Low Dose Radiation Research Program must be available to other scientists, regulatory agencies, and the public. This project stays abreast of scientific advances in the field, gathers, integrates, and summarizes the research within the program, and disseminates this information to appropriate scientific, regulatory, and public venues. Project Goals Provides a focal point for distribution of information generated in the program, to scientific committees, other governmental and regulatory agencies, and to the public Provides scientific support for the Low Dose Program website

145

Low Dose Radiation Research Program: What's New Archive  

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What's New What's New Tony Brooks Chosen As Lauriston S. Taylor Lecturer Congratulations to radiation biologist Dr. Antone "Tony" Brooks, a consultant to the Pacific Northwest National Laboratory's Low Dose Radiation Research Program, on his selection to give the 36th Lauriston S. Taylor Lecture at the Annual Meeting of the National Council on Radiation Protection and Measurements (NCRP). Brooks is former chief scientist for the U.S. Department of Energy's Low Dose Radiation Research Program. His lecture, titled "From the Field to the Laboratory and Back: The What Ifs, Wows, and Who Cares of Radiation Biology," will be a featured presentation at the meeting, which will be held March 12 and 13, 2012, at the Hyatt Regency Bethesda, Bethesda, Maryland.

146

United States Department of Energy Low Dose Radiation Research Program  

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History of the History of the United States Department of Energy (DOE) Low Dose Radiation Research Program: 1998-2008 Dr. Antone L. Brooks tbrooks@tricity.wsu.edu September 2012 Review Draft i Contents Preface............................................................................................................................................. v Summary ........................................................................................................................................ vi Acronyms and Initialisms ............................................................................................................. vii Chapter 1 Introduction ................................................................................................................... 1

147

Low-Dose/Dose-Rate Low-LET Radiation Protects Us from Cancer  

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Dose/Dose-Rate Low-LET Radiation Protects Us from Cancer Dose/Dose-Rate Low-LET Radiation Protects Us from Cancer Bobby R. Scott, Ph.D. and Jennifer D. Di Palma Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive SE Albuquerque, NM 87108 USA Life on earth evolved in a low-level ionizing radiation environment comprised of terrestrial radiation and cosmic rays. Today we all reside in an ionizing radiation environment comprised of both natural background radiation and radiation from human activities (e.g., Chernobyl accident). An evolutionary benefit of the interaction of low-level, low linear-energy-transfer (LET) ionizing radiation with mammalian life forms on earth is adapted protection. Adapted protection involves low-dose/dose-rate, low-LET radiation induced high-fidelity DNA repair in cooperation with normal apoptosis (presumed p53

148

Poly [1,1'-bis(ethynyl)-4,4'-biphenyl(bis-tributylphosphine)Pt(II)] solutions used as low dose ionizing radiation dosimeter  

Science Conference Proceedings (OSTI)

In this work, the effect of gamma radiation on the optical properties of polymetallayne poly[1,1'-bis(ethynyl)-4,4'-biphenyl(bis-tributylphosphine)Pt(II)] (Pt-DEBP) in chloroform solution is studied. The samples were irradiated at room temperature with doses from 0.01 Gy to 1 Gy using a {sup 60}Co gamma ray source. A new band at 420 nm is observed in the emission spectra, in superposition to the emission maximum at 398 nm, linearly dependent on dose. We propose to use the ratio of the emission amplitude bands as the dosimetric parameter. This method proved to be robust, accurate, and can be used as a dosimeter in medical applications.

Bronze-Uhle, E. S.; Graeff, C. F. O. [Department of Physics, FC-UNESP, Av. Eng. Luiz Edmundo Carrijo Coube 14-01, 17033-360 Bauru (Brazil)] [Department of Physics, FC-UNESP, Av. Eng. Luiz Edmundo Carrijo Coube 14-01, 17033-360 Bauru (Brazil); Batagin-Neto, A.; Fernandes, D. M. [UNESP-Univ Estadual Paulista, POSMAT-Programa de Pos-Graduacao em Ciencia e Tecnologia de Materiais, Bauru, Sao Paulo (Brazil)] [UNESP-Univ Estadual Paulista, POSMAT-Programa de Pos-Graduacao em Ciencia e Tecnologia de Materiais, Bauru, Sao Paulo (Brazil); Fratoddi, I.; Russo, M. V. [Department of Chemistry, University of Rome 'Sapienza,' P.le A. Moro 5, 00185 Rome (Italy)] [Department of Chemistry, University of Rome 'Sapienza,' P.le A. Moro 5, 00185 Rome (Italy)

2013-06-17T23:59:59.000Z

149

Low Dose Radiation Research Program: Molecular Mechanisms of  

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Molecular Mechanisms of Radiation-Induced Genomic Instability in Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells. Authors: Howard L. Liber1 and Jeffrey L. Schwartz2. Institutions: 1Colorado State University and 2University of Washington. Knowledge of the signal and target through which radiation induces genomic instability, which we propose to investigate herein, will allow us to model effects at low doses. Such knowledge will aid in understanding variations in the induction of this genomic instability, both among cells and among individuals. This information could also lead to more sensitive measures of instability that could yield accurate measures of instability induction at low doses. We have developed an in-vitro cell model, in which radiation-induced chromosome instability develops in a two-stage process.

150

Low Dose Radiation Research Program: Molecular Characterization of Survival  

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Survival Advantage, Bystander Effect, and Survival Advantage, Bystander Effect, and Genomic Instability after Low-LET Low Dose Radiation Exposure Mohan Natarajan University of Texas Health Science Center Why this Project? To understand the molecular link between the activation of NF-kB and cellular outcomes such as better cell survival after low-LET radiation and to determine whether low dose radiation-induced NF-kB signaling can mediate telomerase activation and thus confer enhanced cell survival of normal aortic endothelial cells. Project Goals To determine whether low-linear energy transfer (LET) radiation can cause a positive feedback signal initiated by the activation of the NF-kB. To examine one of the mechanisms involving TNF-a as a signaling mediator, which could mediate the bystander effect through the generation

151

Low Dose Radiation Research Program: Janet E. Baulch  

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Janet E. Baulch Janet E. Baulch University of California, Davis Currently Funded Projects Impact of Genetic Factors on the Heritable Effects of Paternal Exposure to Low-Dose Radiation Technical Abstracts 2003 Workshop: DNA damage in acutely irradiated F2 mice with a history of paternal F0 germline irradiation Baulch, J.E. and Raabe, O G. 2002 Workshop Impact of Genetic Factors on the Heritable Effects of Paternal Exposure to Low-Dose Radiation, Baulch, J.E., Ph.D. and Raabe, O.G., Ph.D. Publications Kovalchuk, O. and Baulch, J.E. (2008). Epigenetic changes and nontargeted radiation effects - Is there a link? Environmental and Molecular Mutagenesis 49(1):16-25 Laiakis, E.C., Baulch, J.E., and Morgan, W.F. (2008). Interleukin 8 exhibits a pro-mitogenic and pro-survival role in radiation induced

152

The Mechanism of Low Dose Radiation Risk Associated with Diagnostic X-rays,  

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Mechanism of Low Dose Radiation Risk Associated with Diagnostic X-rays, Mechanism of Low Dose Radiation Risk Associated with Diagnostic X-rays, PET, and Gamma-rays Douglas Boreham McMaster University Abstract The goal of this project was to investigate low dose ionizing radiation effects associated with exposure to diagnostic computed tomography (CT) or positron emission tomography (PET) scans. Biological effects were evaluated in wild type and Trp53+/- heterozygous females, following in vivo exposure to diagnostic CT (75kVp, 200µ) or PET (18F-FDG) scans. The short term biological effects following CT or PET scans were evaluated in order to understand biological modification of mechanisms, such as DNA repair processes and apoptosis, that might alter long term cancer risk. Corresponding life-time cancer risk studies are in progress. Short-term

153

Low Dose Radiation Research Program: The Role of the Number and Spacing of  

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Program Workshop I Program Workshop I November 10-12, 1999, Washington, D.C. The Role of the Number and Spacing of Electron Tracks on the Consequences of Low Dose Irradiation Leslie A. Braby and J. R. Ford Nuclear Engineering, Texas A&M University, 129 Zachry, College Station, Texas. Summary: Biological mechanisms, which may influence the health risks resulting from very low dose radiation exposures, will be investigated using a collimated beam of electrons to simulate the irradiation patterns occurring with low dose exposures. Abstract: Ionizing radiation produces a variety of free radicals and chemical products that react to produce the same types of oxidative damage in a mammalian cell as produced by the normal metabolic activity of the cell. However, the damage produced by radiation is distributed differently

154

Low Dose Radiation Research Program: Wide Expression of LLIR and the  

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Wide Expression of LLIR and the Biological Consequences Wide Expression of LLIR and the Biological Consequences David J. Chen Lawrence Berkeley National Laboratory Why This Project It is known that changes in gene expression alter biological effects. It is necessary to identify the specific genes that demonstrate altered expression after exposure to low doses of ionizing radiation and to determine pathways involved in DNA damage recognition, signaling, and repair that are associated with radiation-induced adaptive and bystander effects. Project Goals Identification of genes whose transcription is regulated in response to low levels of ionizing radiation Identification of the genes and communication pathways that control these responses to low dose radiation Identification of the cellular and molecular targets that influence

155

Molecular Mechanisms of Low Dose Radiation Mediated Hormesis in C. elegans  

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Mechanisms of Low Dose Radiation Mediated Hormesis in C. Mechanisms of Low Dose Radiation Mediated Hormesis in C. elegans Anders Olsen, Maithili C. Vantipalli, Arnold Kahn, Judith Campisi and Gordon J. Lithgow. Buck Institute for Age Research, 8001 Redwood Boulevard, Novato CA 94945 Brief exposure to a mild stress causes induction of stress gene expression leading to enhanced stress responses, improved maintenance and repair and in some cases lifespan increase. This phenomenon is termed hormesis and has been observed in several species. For example, we previously demonstrated that short periods of mild heat stress in early life increase both mean and maximum lifespan of the soil nematode C. elegans. Similar hormetic responses have been described for many other stressors. Here we present data showing that treatment of the nematode with low-doses of ionizing

156

Effects of low-dose radiation on immune cell function using genetic and  

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low-dose radiation on immune cell function using genetic and low-dose radiation on immune cell function using genetic and metabolomics approaches Henghong Li Georgetown University Abstract The objectives of this study are to investigate acute and persistent effects of ionizing radiation and space radiation on immune cell subsets and function. The role(s) for p38 MAP kinase in such radiation responses is being investigated using a genetic approach where an engineered mouse line has had one wt p38α gene replaced with a dominantnegative mutant (p38α+/DN). T cells are one of the most radiosensitive cell types in vivo, and radiation is known to impact CD4 T cell function long term. T cells are normally activated by antigen, which triggers differentiation to specific subsets involving various cytokines. In addition, T cells have a

157

DOE Low Dose Radiation Research Workshop I November 1999  

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Low Dose Radiation Research Program Low Dose Radiation Research Program U.S. Department of Energy Office of Biological and Environmental Research David G. Thomassen, Ph.D. Program Coordinator Office of Biological and Environmental Research U.S. Department of Energy, SC-72 19901 Germantown Road Germantown, MD 20874-1290 Phone: 301-903-9817 Fax: 301-903-8521 Email: david.thomassen@science.doe.gov Arthur Katz, Ph.D. Life Sciences Division Office of Biological and Environmental Research U.S. Department of Energy, SC-72 19901 Germantown Road Germantown, MD 20874-1290 Phone: 301-903-4932 Fax: 301-903-8521 Email: arthur.katz@science.doe.gov Marvin E. Frazier, Ph.D. Director, Life Sciences Division Office of Biological and Environmental Research U.S. Department of Energy, SC-72 19901 Germantown Road

158

Low Dose Radiation Research Program: Biologically Based Analysis of Lung  

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Biologically Based Analysis of Lung Cancer Incidence in a Large Biologically Based Analysis of Lung Cancer Incidence in a Large Canadian Occupational Cohort with Low-LET Low-dose Radiation Exposure, and Comparison with Japanese Atomic Bomb Survivors. Authors: W.D. Hazelton, D. Krewski, S.H. Moolgavkar Lung cancer incidence is analyzed in a large Canadian National Dose Registry (CNDR) cohort with individual annual dosimetry for low-dose occupational exposure to gamma and tritium radiation using several types of multistage models. The primary analysis utilizes the two-stage clonal expansion model (TSCE), with sensitivity analyses using extensions of this model incorporating additional stages. Characteristic and distinct temporal patterns of risk are found for dose-response affecting early, middle, or late stages of carcinogenesis, e.g., initiation with one or more stages,

159

Low Dose Radiation Research Program: Optimizing the Scientific, Regulatory  

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Optimizing the Scientific, Regulatory and Social Impact of the DOE Optimizing the Scientific, Regulatory and Social Impact of the DOE Low Dose Radiation Research Program. Authors: Antone L.Brooks, Richard J. Bull, Lezlie A. Couch. Institutions: Washington State University Tri-Cities The purpose of this project is to provide scientific, technical, and organizational support to optimize the impact of the DOE Low Dose Radiation Research Program. This project will serve as a focal point for collection and dissemination of scientific information from the scientists funded in the Program to the U.S. Department of Energy (DOE), the regulatory agencies, and the public. The project will be responsible for analysis of the scientific information in the broader context of biomedical research and will provide this information to the Office of Biological Research

160

Low Dose Radiation Research Program: Studies of Low-Dose Bystander...  

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Low-Dose Bystander Effects Using a Focused Soft X-ray Microprobe Kevin M. Prise1, Melvyn Folkard1, Giuseppe Schettino1, Elena Rusyn2, Heidi C. Newman1, Kathryn D. Held2 and Barry...

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Low Dose Radiation Research Program: Quantification of Repair of  

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Quantification of Repair of Low-Dose-Induced DNA Double-Strand Quantification of Repair of Low-Dose-Induced DNA Double-Strand Breaks in Diploid Human Cells Authors: David Schild,1 and Larry H. Thompson,2 Institutions: 1Life Sciences Division, Lawrence Berkeley National Laboratory; and 2BBR Program, Lawrence Livermore National Laboratory Double-strand breaks (DSBs) are the biochemical lesions of primary concern in radiation related health effects. Compelling evidence from rodent and chicken model systems indicates that homologous recombinational repair (HRR) plays an essential role for cell viability in the repair of spontaneous DSBs arising during DNA replication and an important role in the repair of IR-induced DSBs. IR-induced DSBs are also repaired by error-prone nonhomologous end joining (NHEJ). Using hTERT-immortalized

162

Low Dose Radiation Research Program: Optimizing the Scientific, Regulatory  

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Optimizing the Scientific, Regulatory and Societal Impact of the DOE Optimizing the Scientific, Regulatory and Societal Impact of the DOE Low Dose Research Program Authors: Antone L. Brooks Institution: Washington State University Tri-Cities Richland, Washington The purpose of this project is to provide a focal point for communication of the research results from the DOE Low Dose Radiation Research Program. The major communication tool provided by this project is a Website at Washington State University. The website is being maintained to provide communication between the scientific advances generated by the research program and scientists both in and outside the program, policy makers, regulators and the public. The website also contains a number of presentations and illustrations that are written so that they will be easy

163

Low Dose Radiation Research Program Website ? Highlighting Low...  

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Research Program Website - Highlighting Low Dose Research Bill Morgan, Principal Investigator; Julie Wiley, Website Content Manager; Christine Novak, Webmaster The Low Dose...

164

Low Dose Radiation Program: Links - Current Issues Involving...  

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of Ionizing Radiation A-Bombs Atomicarchive.com Hiroshima Peace site History of the Atomic Bomb & The Manhattan Project The Atomic Testing Museum The Race to Build the Atomic...

165

Low Dose Radiation Research Program: Mary Helen Barcellos-Hoff - 2003  

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DOE Low Dose Radiation Program Workshop IV DOE Low Dose Radiation Program Workshop IV Abstract Title: TGF-β Protects Human Mammary Epithelial Cells from Radiation-Induced Centrosome Amplification Authors: Mary Helen Barcellos-Hoff, Bahram Parvin, Anna C. Erickson and Rishi Gupta Institutions: Department of Cell and Molecular Biology, Life Sciences Division, Ernest Orlando Lawrence, Berkeley National Laboratory, Berkeley, California In recent studies we have shown that ionizing radiation (IR), a known carcinogen of human and murine mammary gland, compromises human mammary epithelial cell (HMEC) polarity and multicellular organization in a manner characteristic of neoplastic progression through a heritable, non-mutational mechanism (1). Thus, when all cells are irradiated with a significant dose (2 Gy), the daughters of irradiated cells lose their

166

Low Dose Radiation Research Program: Role of TNF-α as a Potential  

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Role of TNF-α as a Potential Signaling Mediator of Role of TNF-α as a Potential Signaling Mediator of Radiation-Induced Bystander Effect in Human Vascular Cells. Authors: Mohan Natarajan, Sumathy Mohan, Catherine Gibbons, Yan Bo and Munira A. Kadhim Institutions: Departments of Radiation Oncology and Pathology, University of Texas Health Science Center, San Antonio, Texas; Environmental Toxicology Graduate Program, University of California, Riverside; Radiation and Genomic Stability Unit, Medical research Council, Oxford, United Kingdom Identifying reliable and sensitive signaling pathways that are implicated in adverse health effects after exposure to low doses of ionizing radiation would allow us to understand the scientific basis of low dose-induced signaling pathways and their downstream phenotypic expression. This

167

Low Dose Radiation Research Program: Robert L. Ullrich  

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Robert L. Ullrich Robert L. Ullrich Colorado State University Currently Funded Projects Radiation Leukemogenesis at Low Dose Rates (NSCOR) Genetic Mechanisms of Induced Chromosomal Instability and their Relationships with Radiation Tumorigenesis Technical Abstracts 2006 Workshop: The Role of Telomere Dysfunction in Driving Genomic Instability Bailey, S.M., Williams, E.S., and Ullrich, R.L. 2005 Workshop: Dsyfunctional Mammalian Telomeres in DNA-PKcs Deficient Backgrounds Bailey, S.M., Williams, E., Hagelstrom, T., and Ullrich, R.L. 2003 Workshop: Dysfunctional Mammalian Telomeres Join to Double-Strand Breaks Bailey, S.M., Goodwin, E.H., Williams, E., and Ullrich, R.L. 2002 Workshop: Dysfunctional Telomeres, Radiation-Induced Instability and Tumorigenesis Bailey, S.M., Goodwin, E.H., Cornforth, M.N., and Ullrich, R.L.

168

Low Dose Radiation Research Program: Research Highlights - Collateral  

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Collateral Damage Collateral Damage Using targeted irradiation to understand radiation-induced effects in bystander cells chromosomal A typical example of chromosomal instability induction measured by chromosome-type aberrations in primary human lymphocytes at delay time post-irradiation of a fraction of the cell population. Similar types of aberrations were observed in whole irradiated population but were not observed in untreated cells. Munira Kadhim Background: It has long been understood that radiation exposure can influence cellular changes. Studies indicate that even very low doses of high linear energy transfer (LET) alpha-particle irradiation, such as that from environmental radon, can affect cells. Radiation-induced genomic instability can be observed in the progeny of irradiated cells and as a

169

Low Dose Radiation Research Program: Rainer K. Sachs  

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Rainer K. Sachs Rainer K. Sachs University of California, Berkeley Funded Projects BIO-BASED RISK MODELING 03-20: Modeling the Interrelations Among Radiation-Induced Bystander Effects, Genomic Instability and Cancer Cytogenetic Tests of Radiobiological Models Relating Epidemiologically Measurable Risks to Low-Dose Risks Technical Abstracts 2006 Workshop The Bystander Effect in Normal Human 3-D Tissue: Experiments, Models, and Implications Brenner, D., Ponnaiya, B., Shuryak, I., Sachs, R., and Geard, D. Radiation Carcinogenesis Risk as Influenced by Intercellular Interaction Hahnfeldt, P., Hlatky, L., and Sachs, R.K. 2005 Workshop: Modelling Intercellular Interactions During Radiation Carcinogenesis Sachs, R.K., Chan, M., Hlatky, L., and Hahnfeldt, P. 2003 Workshop: Chromosome Spatial Clustering Uncovered Through Radiogenic Aberrations

170

Low Dose Radiation Research Program: Mechanisms of Tissue Response to Low  

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Tissue Response to Low Dose Radiation Tissue Response to Low Dose Radiation Mary Helen Barcellos-Hoff Lawrence Berkeley National Laboratory Why This Project? In the past, the effects of ionizing radiation on humans has been attributed in great part to its ability to damage DNA, which transmits information from cell to cell, and generation to generation. Damaged DNA can lead to cell death or perpetuate the damage to daughter cells and to future generations. In addition to the information contained with the genome (i.e., DNA sequence), information directing cell behavior and tissue function is also stored outside the DNA. The success in cloning sheep from the DNA contained in the nucleus of an adult cell shows how important signals from the outside are in defining how the genome is expressed. This

171

Low Dose Radiation Program: Links - General Radiation Information  

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General Radiation Information Answers to Questions about Radiation Dose Ranges Charts - tables showing radiation dose ranges from radio diagnostics to cancer radiotherapy....

172

Low Dose Radiation Program: Links - Websites about Radiation  

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Websites About Radiation The ABC's of Nuclear Science A Teacher's Guide To The Nuclear Science Wall Chart Answers to Questions about Radiation and You Background Radiation:...

173

Low Dose Radiation Research Program: Regulation of NF-kB and MnSOD in Low  

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NF-kB and MnSOD in Low Dose Radiation-Induced Adaptive NF-kB and MnSOD in Low Dose Radiation-Induced Adaptive Responses in Mouse and Human Skin Cells Jian Jian Li School of Health Sciences, Purdue University West Lafayette, Indiana Why this Project? To determine if low dose ionizing radiation-induced adaptive responses in skin cells are mediated by activation of signaling networks. Project Goals To evaluate the signaling networks involving transcription factor NF-kB and the mitochondrial antioxidant protein MnSOD. To determine if NF-kB is activated by low dose radiation in vivo. To determine if NF-kB activation is critical in the pathways that produce adaptive responses. Experimental Approach Cells transfected with NF-kB luciferase responder genes will be used to define a dose-response relationship for activation of the NF-kB gene. NF-kB

174

Low Dose Radiation Research Program: Characterizing Bystander Effects  

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Irradiation. Irradiation. Authors: L.A. Braby and J.R. Ford. Institutions: Texas A&M University. Bystander effects, which are typically seen as in increase in the cellular concentration of specific repair related molecules or as cytogenetic changes which appear to be the consequence of DNA damage, may be a significant factor in the risk of long-term health effects of low doses of radiation. These effects clearly increase the effective size of the target for radiation response, from the diameter of a single cell or cell nucleus to something significantly larger, by bringing additional cells into the process. It is unclear whether this larger target will result in an increase or a decrease in the probability of inducing a change which would be detrimental to the health of the organism, but it clearly reduces the

175

Low Dose Radiation Research Program: Genetic Mechanisms of Induced  

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Mechanisms of Induced Chromosomal Instability and their Mechanisms of Induced Chromosomal Instability and their Relationships with Radiation Tumorigenesis Robert Ullrich Colorado State University Why This Project A combination of epidemiological, experimental, animal, and cellular molecular data is used in the estimation of tumor risk after low doses of low-LET radiation. Uncertainties are recognized in the interpretation of all these data sets, and recent findings concerning genomic instability in irradiated cells challenge the conventional view that induced DNA damage is expressed during the immediate post-irradiation cell cycle. These data on genomic instability are based largely upon studies of cell cultures the mechanisms involved and implications for tumor formation in living organisms remain unclear. Nevertheless, if induced genomic instability were

176

Low Dose Radiation Research Program: Comparisons of IR and ROS for  

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Comparisons of IR and ROS for Induction of Damage to Cells Comparisons of IR and ROS for Induction of Damage to Cells Kathryn D. Held1, Yvonne L. McCarey1, Laurence Tartier1, Elena V. Rusyn1, Giuseppe Schettino2, Melvyn Folkard2, Kevin M. Prise2, and Barry D. Michael2 1Department of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114; 2Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, HA6 2JR, UK Accurate evaluation of the risks associated with exposure to low doses of ionizing radiation (IR) is a major challenge for environmental sciences. Studies on the mechanisms of the actions of low doses of IR are needed to help understand possible risks. IR exerts its effects on cells through production of reactive oxidizing species (ROS) such as ·OH, H2O2 and

177

Low Dose Radiation Program: Links - Organizations Funding Radiation...  

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Funding Radiation Research Australian Radiation Protection and Nuclear Safety Agency Canadian Nuclear Safety Commission Centers for Medical Countermeasures Against Radiological and...

178

Low Dose Radiation Program: Links - Agencies with Radiation Regulatory  

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Agencies with Radiation Regulatory Concerns and Involvement Agencies with Radiation Regulatory Concerns and Involvement Biological Effects of Low Level Exposures (BELLE) Canadian Nuclear Safety Commission Center for Risk Excellence Health Protection Agency The Health Risks of Extraterrestrial Environments International Commission on Radiation Units and Measurements, Inc. International Commission on Radiological Protection (ICRP) International Radiation Protection Association (IRPA) NASA Space Radiation Program National Academy of Sciences (NAS) Nuclear and Radiation Studies Board National Aeronautics and Space Administration (NASA) NASA OBRR Task Book Publication National Council on Radiation Protection (NCRP) National Institute of Environmental Health Sciences (NIEHS) National Toxicology Program (NTP) Occupational Safety and Health Administration (OSHA)

179

Low Dose Radiation Research Program: Past Funded Projects-Archive  

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Berkeley, CA A Quantitative Assessment of Bystander Mutagenesis in the Mouse Mammary Gland In Vivo Bruce E. Lehnert Los Alamos National Laboratory, Los Alamos, NM Low Dose...

180

Low Dose Radiation Research Program: Quantification of Repair...  

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Quantification of Repair of Low-Dose-Induced DNA Double-Strand Breaks in Diploid Human Cells David Schild Lawrence Berkeley National Laboratory Berkeley, California Why this...

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Low Dose Radiation Program: Links - Research Societies with Radiation...  

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Societies with Radiation Concerns Academy of Radiology Research American Association of Physicists in Medicine American Nuclear Society American Roentgen Ray Society American...

182

Low Dose Radiation Research Program: Radiation Response in Normal...  

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genes. Using rigorous computational methods, we characterized the dose-dependent, radiation-induced gene expression of HSF-42, a primary cell culture. Our preliminary results...

183

Low Dose Radiation Research Program: Frequencies of Radiation-Induced  

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Frequencies of Radiation-Induced Chromosome Interchanges and Frequencies of Radiation-Induced Chromosome Interchanges and Randomness of Chromosome Territory Locations Relative to One Another. Authors: RK Sachs,§ MN Cornforth,‡ KM Greulich-Bode,* L Hlatky, and DJ Brenner|| Institutions: §Department of Mathematics, University of California, ‡University of Texas Medical Branch, *Department of Skin Carcinogenesis, German Cancer Research Center DFCI, Harvard Medical School, ||Center for Radiological Research, Columbia University. Leukemogenesis, and perhaps carcinogenesis in general, often involves specific chromosome translocations. Radiation-induced chromosome translocation frequencies are strongly influenced by how close participating chromosomes are to one another in an interphase cell nucleus. We sought to determine whether chromosomes in human peripheral blood

184

Low Dose Radiation Research Program: Research Highlights - 2010  

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DNA Double-Strand Break Repair Capacities Before and After Exposure to Low DNA Double-Strand Break Repair Capacities Before and After Exposure to Low Dose Radiation Immunochemical detection of DSB foci in the nuclei of human fibroblasts. (A) γ-H2AX phospho-serine 139 foci (chromatin marker of DSBs; green). (B) ataxia-telangiectasia mutated phospho-serine 1981 foci (ATM, DNA damage-responsive kinase; red). (C) Merge of images A and B. (White arrows mark large γ-H2AX foci and coincident γ-H2AX/pATM foci that were positively scored; yellow arrows mark small γ-H2AX foci that lack corresponding pATM foci that were not scored.) Enlarge Image Immunochemical detection of DSB foci in the nuclei of human fibroblasts. (A) γ-H2AX phospho-serine 139 foci (chromatin marker of DSBs; green). (B) ataxia-telangiectasia mutated phospho-serine 1981 foci (ATM,

185

Low Dose Radiation Research Program: Biological Response of Individual...  

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Response of Individual Cells Following Electron Microbeam Irradiation L. A. Braby and J. R. Ford Texas A&M University, College Station Texas In recent years studies with low doses...

186

Low Dose Radiation Research Program: Techniques and Technology  

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Role of the Number and Spacing of Electron Tracks on the Consequences of Low Dose Irradiation X-ray microfocus source The new X-ray microfocus source Enlarge Image. Melvyn...

187

THE ROLES OF SUPEROXIDE DISMUTAGE (SOD) IN LOW DOSE RADIATION...  

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the detoxifying enzymes may be even more prominent in the case of low-dose, low-LET irradiation, as the majority of genetic damage may be caused by secondary oxidative species. In...

188

Radiosensitization of Human Cervical Cancer Cells by Inhibiting Ribonucleotide Reductase: Enhanced Radiation Response at Low-Dose Rates  

Science Conference Proceedings (OSTI)

Purpose: To test whether pharmacologic inhibition of ribonucleotide reductase (RNR) by 3-aminopyridine-2-carboxaldehyde thiosemicarbazone (3-AP, NSC no. 663249) enhances radiation sensitivity during low-dose-rate ionizing radiation provided by a novel purpose-built iridium-192 cell irradiator. Methods and Materials: The cells were exposed to low-dose-rate radiation (11, 23, 37, 67 cGy/h) using a custom-fabricated cell irradiator or to high-dose-rate radiation (330 cGy/min) using a conventional cell irradiator. The radiation sensitivity of human cervical (CaSki, C33-a) cancer cells with or without RNR inhibition by 3-AP was evaluated using a clonogenic survival and an RNR activity assay. Alteration in the cell cycle distribution was monitored using flow cytometry. Results: Increasing radiation sensitivity of both CaSki and C33-a cells was observed with the incremental increase in radiation dose rates. 3-AP treatment led to enhanced radiation sensitivity in both cell lines, eliminating differences in cell cytotoxicity from the radiation dose rate. RNR blockade by 3-AP during low-dose-rate irradiation was associated with low RNR activity and extended G{sub 1}-phase cell cycle arrest. Conclusions: We conclude that RNR inhibition by 3-AP impedes DNA damage repair mechanisms that rely on deoxyribonucleotide production and thereby increases radiation sensitivity of human cervical cancers to low-dose-rate radiation.

Kunos, Charles A., E-mail: charles.kunos@UHhospitals.org [Department of Radiation Oncology, University Hospitals Case Medical Center and Case Western Reserve School of Medicine, Cleveland, OH (United States); Colussi, Valdir C. [Department of Radiation Oncology, University Hospitals Case Medical Center and Case Western Reserve School of Medicine, Cleveland, OH (United States); Pink, John [Department of General Medical Sciences, University Hospitals Case Medical Center and Case Western Reserve School of Medicine, Cleveland, OH (United States); Radivoyevitch, Tomas [Department of Epidemiology and Biostatistics, Case Comprehensive Cancer Center, University Hospitals Case Medical Center and Case Western Reserve School of Medicine, Cleveland, OH (United States); Oleinick, Nancy L. [Department of Radiation Oncology, University Hospitals Case Medical Center and Case Western Reserve School of Medicine, Cleveland, OH (United States)

2011-07-15T23:59:59.000Z

189

Effect of Low Dose Radiation on Antioxidant Levels in Rat Brain  

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Low Dose Radiation on Antioxidant Levels in Rat Brain Mohan Doss Fox Chase Cancer Center Abstract Background: Parkinsons disease (PD) is characterized by progressive...

190

Characterization of the role of Fhit in maintenance of genomic integrity following low dose radiation, in vivo and in vitro  

Science Conference Proceedings (OSTI)

The major goal of this study is to determine the effects of the Fhit pathway on low dose ({le} 0.1 Gy) ionizing radiation (IR)-induced genetic instability. Reduction of Fhit protein expression is observed in most solid tumors particularly in those tumors resulting from exposure to environmental carcinogens. Therefore, characterization of the role of the Fhit-dependent pathway in preventing low dose IR-induced genetic instability will provide useful parameters for evaluating the low dose IR-induced risk of mutagenesis and carcinogenesis. We pursued 3 specific aims to study our hypothesis that the Fhit-dependent pathways maintain genomic integrity through adjusting checkpoint response and repair genes expression following low dose IR. Aim 1: Determine whether Fhit interaction with RPA is necessary for Fhit to affect the cellular response to low dose IR. We combined the approaches of in vitro (GST pull-down and site-directed mutagenesis) and in vivo (observing the co-localization and immunoprecipitation of Fhit and RPA in Fhit knock out mouse cells transfected with mutant Fhit which has lost ability to interact with RPA in vitro). Aim 2: Determine the role of genes whose expression is affected by Fhit in low dose irradiated cells. We analyzed the distinct signature of gene expression in low dose irradiated Fhit-/- cells compared with Fhit+/+ cells by combining microarray, gene transfection and siRNA approaches. Aim 3: Determine the role of Fhit in genetic susceptibility to low dose IR in vivo. We compared the gene mutation frequency and the fragile site stability in the cells isolated from the Fhit+/+ and Fhit-/- mice at 1.5 years following low dose IR. These results determine the role of the Fhit-dependent pathway in maintaining genomic integrity in vitro and in vivo, which provide a basis for choosing surrogate markers in the Fhit-dependent pathway to evaluate low dose IR-induced risk of mutagenesis and carcinogenesis.

Ya Wang

2010-05-31T23:59:59.000Z

191

Cellular response to low dose radiation: Role of phosphatidylinositol-3 kinase like kinases  

Science Conference Proceedings (OSTI)

It is increasingly realized that human exposure either to an acute low dose or multiple chronic low doses of low LET radiation has the potential to cause different types of cancer. Therefore, the central theme of research for DOE and NASA is focused on understanding the molecular mechanisms and pathways responsible for the cellular response to low dose radiation which would not only improve the accuracy of estimating health risks but also help in the development of predictive assays for low dose radiation risks associated with tissue degeneration and cancer. The working hypothesis for this proposal is that the cellular mechanisms in terms of DNA damage signaling, repair and cell cycle checkpoint regulation are different for low and high doses of low LET radiation and that the mode of action of phosphatidylinositol-3 kinase like kinases (PIKK: ATM, ATR and DNA-PK) determines the dose dependent cellular responses. The hypothesis will be tested at two levels: (I) Evaluation of the role of ATM, ATR and DNA-PK in cellular response to low and high doses of low LET radiation in simple in vitro human cell systems and (II) Determination of radiation responses in complex cell microenvironments such as human EpiDerm tissue constructs. Cellular responses to low and high doses of low LET radiation will be assessed from the view points of DNA damage signaling, DNA double strand break repair and cell cycle checkpoint regulation by analyzing the activities (i.e. post-translational modifications and kinetics of protein-protein interactions) of the key target proteins for PI-3 kinase like kinases both at the intra-cellular and molecular levels. The proteins chosen for this proposal are placed under three categories: (I) sensors/initiators include ATM ser1981, ATR, 53BP1, gamma-H2AX, MDC1, MRE11, Rad50 and Nbs1; (II) signal transducers include Chk1, Chk2, FANCD2 and SMC1; and (III) effectors include p53, CDC25A and CDC25C. The primary goal of this proposal is to elucidate the differences in cellular defense mechanisms between low and high doses of low LET radiation and to define the radiation doses where the cellular DNA damage signaling and repair mechanisms tend to shift. This information is critically important to address and advance some of the low dose research program objectives of DOE. The results of this proposed study will lead to a better understanding of the mechanisms for the cellular responses to low and high doses of low LET radiation. Further, systematic analysis of the role of PIKK signaling pathways as a function of radiation dose in tissue microenvironment will provide useful mechanistic information for improving the accuracy of radiation risk assessment for low doses. Knowledge of radiation responses in tissue microenvironment is important for the accurate prediction of ionizing radiation risks associated with cancer and tissue degeneration in humans.

Balajee, A.S.; Meador, J.A.; Su, Y.

2011-03-24T23:59:59.000Z

192

Low Dose Radiation Research Program: DNA Damage in Acutely Irradiated F2  

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DNA Damage in Acutely Irradiated F2 Mice with a History of Paternal DNA Damage in Acutely Irradiated F2 Mice with a History of Paternal F0 Germline Irradiation Authors: J.E. Baulch and O.G. Raabe Institutions: Center for Health and the Environment, University of California, Davis, CA. The main goal of this grant is to evaluate heritable, transgenerational effects of low dose, low-linear-energy-transfer (LET) radiation (0.1 Gy attenuated 137Cs gamma rays) on Type B spermatogonia in 129SVE mice; wild-type and heterozygous for Ataxia-telangiectasia (AT). The ATM heterozygotes are carriers for a genetic mutation (AT mutated, ATM) that is thought to predispose both humans and mice to radiation sensitivity. Experiments conducted in our laboratory have demonstrated heritable effects of paternal germline exposure to ionizing radiation in mice using 1.0 Gy of

193

An evaluation of theories concerning the health effects of low-dose radiation exposures  

E-Print Network (OSTI)

The danger of high, acute doses of radiation is well documented, but the effects of low-dose radiation below 100 mSv is still heavily debated. Four theories concerning the effects of lowdose radiation are presented here: ...

Wei, Elizabeth J. (Elizabeth Jay)

2012-01-01T23:59:59.000Z

194

Low-Dose Radiation Cataract and Genetic Determinants of Radiosensitivity  

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Cataract and Genetic Determinants of Radiosensitivity Cataract and Genetic Determinants of Radiosensitivity Kleiman, N.J. 1 , Smilenov, L.B. 2 , Brenner, D.J. 2 and Hall, E.J. 2 1 Department of Environmental Health Sciences, Mailman School of Public Health & 2 The Center for Radiological Research, College of Physicians & Surgeons, Columbia University, New York 10032 The lens of the eye is one of the most radiosensitive tissues in the body. Ocular ionizing radiation exposure results in characteristic, dose related, progressive lens changes leading to cataract formation. While initial, early stages of such opacification may not cause visual disability, the severity of such changes progressively increases with dose until vision is impaired and cataract extraction surgery may be required. The

195

Low Dose Radiation Research Program: Quantitative Analysis of Connexin  

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Quantitative Analysis of Connexin Expression in Cultured Colonies Quantitative Analysis of Connexin Expression in Cultured Colonies Authors: B. Parvin, Q. Yang, R. L. Henshall-Powell and M.H. Barcellos Hoff We are studying the effects of ionizing radiation on the signaling between human mammary epithelial cells and the extracellular microenvironment. To do so we use an assay based on the ability of the cells to organize into three-dimensional acini when embedded into an extracellular matrix. Although tumorigenic and non-tumorigenic mammary epithelial cells are nearly indistinguishable when cultured as monolayers, their biological character readily diverge when tissue-specific morphogenesis is analyzed. Non-malignant human mammary epithelial cells (HMEC) cultured within a reconstituted basement membrane organize into acinar-like structures with

196

Low Dose Radiation Research Program: Research Highlights - Real-time  

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Real-time imaging of repair processes possible with Nickel-63 Real-time imaging of repair processes possible with Nickel-63 microirradiator Microscope Microscope stage-mounted microirradiation system. (A) Overall system shown mounted on a micromanipulator housed within a heated, humidified, environmental chamber of a microscope. (B) Close-up showing attachment of device to the micromanipulator. (C) Detail showing the bend in the enclosing capillary, which allows positioning of the active surface directly above the target cell. (D) Microirradiator tip in dissecting microscope. (E) Close-up view through microscope optics (scale bar, 10 microns.) Background: Scientists know that mammalian cells begin a nucleoplasmic repair process within seconds after being exposed to ionizing radiation. Real-time imaging of this process could further understanding of the

197

Low Dose Radiation Research Program: Gene Expression Profile...  

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human cDNA clones, we focused on differential gene expression for a low-dose x-ray irradiation at 2cGy and its comparison with high-dose at 4Gy. Four time points were studied at...

198

The risk of leukemia from low doses of low-LET radiation  

Science Conference Proceedings (OSTI)

In this communication, we examine the evidence (if any) for a nonlinear dose response in relation to leukemia mortality in the Japanese A-bomb population. Specifically, we seek an estimate of the probability that, at low doses of radiation, the relative ... Keywords: A-bomb survivors, Hormesis, Leukemia risk, Low doses of radiation

M. Zaider

2001-06-01T23:59:59.000Z

199

Profiling of MnSOD Interaction Proteins in Low-Dose Radiation...  

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Proteins in Low-Dose Radiation Induced Adaptive Response Angela Eldridge 1 , Ming Fan 1 , Demet Candas 1 , Brett Chromy, and Jian Jian Li 1 1 Department of Radiation...

200

Low Dose Radiation | U.S. DOE Office of Science (SC)  

Office of Science (SC) Website

Radiobiology: Low Dose Radiation Radiobiology: Low Dose Radiation Research Biological and Environmental Research (BER) BER Home About Research Research Abstracts Searchable Archive of BER Highlights External link Biological Systems Science Division (BSSD) Genomic Science DOE Bioenergy Research Centers Radiochemistry & Imaging Instrumentation Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Structural Biology DOE Joint Genome Institute Climate and Environmental Sciences Division (CESD) Facilities Science Highlights Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) News & Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3251 F: (301)

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they are not comprehensive nor are they the most current set.
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to obtain the most current and comprehensive results.


201

Low Dose Radiation Research Program: Induction of Genomic Instability...  

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Brook Why This Project Genomic instability is an important step in radiation-induced cancer. We will investigate one potential repair mechanism involved in radiation-induced...

202

Low Dose Radiation Research Program: Communicating the Science of the Low  

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Communicating the Science of the Low Dose Radiation Research Program Communicating the Science of the Low Dose Radiation Research Program Authors: John S. Wassom, Elizabeth T. Owens, Sheryl A. Martin, Amy K. Wolfe, Margaret K. Lyday,* and Susan L. Dimmick** Institutions: Oak Ridge National Laboratory, *Keener Communications, and the **University of Tennessee Graduate School of Medicine. Summary The project team developed a communications plan based on an explicit communications strategy. The plan presents a set of strategic goals, identifies categories of stakeholders relevant to the program, and suggests methods that can be used to achieve strategic goals and reach targeted stakeholders. Context is key to the communication plan. Providing contextual information about low dose radiation, radiation biology, and Low Dose Radiation

203

Mechanism underlying mTOR-associated Protection in Low Dose Radiation...  

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low-dose radiation-induced adaptive resistance. Oncogene 27: 6738-6748. 2. Gwinn DM, Shackelford DB, Egan DF, Mihaylova MM, Mery A, Vasquez DS, Turk BE, and Shaw RJ. (2008). AMPK...

204

LOW DOSE PHOTON RADIATION-INDUCED CHANGES IN T HELPER LYMPHOCYTES  

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LOW DOSE PHOTON RADIATION-INDUCED CHANGES IN T HELPER LYMPHOCYTES LOW DOSE PHOTON RADIATION-INDUCED CHANGES IN T HELPER LYMPHOCYTES Daila S. Gridley 1,2 , Asma Rizvi 2 , Xian Luo 1 , Adeola Y. Makinde 2 , Steve Rightnar 1 , Jian Tian 1 , Melba L. Andres 1 , James M. Slater 1 , and Michael J. Pecaut 1,2 Departments of 1 Radiation Medicine and 2 Biochemistry & Microbiology Loma Linda University and Medical Center, Loma Linda, CA 92354 USA Health risks due to protracted low dose irradiation remain unclear. This project investigates T helper (Th) lymphocyte function and the cellular milieu in which they reside under conditions of low dose, low- linear energy transfer (LET) radiation exposure. The Th cells are important because they secrete cytokines essential for generating optimal immune defenses against tumor, virus-infected, and other

205

Low Dose Radiation Research Program: Identification of Mouse Genetic  

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Mouse Genetic Susceptibility to Radiation Carcinogenesis Mouse Genetic Susceptibility to Radiation Carcinogenesis Allan Balmain University of California, San Francisco San Francisco, CA. (Jointly funded by NASA and DOE) Why this Project? To identify pathways that control genetic susceptibility to radiation-induced DNA damage and tumor development using novel developments in genomics together with mouse genetics. Project Goals To identify genetic loci that trigger rapid tumor development of mice after radiation. To characterize new genes at these loci that act as tumor suppressor genes or oncogenes. Experimental Approach New candidate-radiation susceptibility genes will be identified using a unique haplotyping approach. Using DNA from radiation-induced lymphoma, changes in the gene copy number can be detected using BAC microarrays. The

206

Low Dose Radiation Research Program: Ionizing Radiation-induced...  

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p53-directed, DNA damage response pathway. The p53 protein, activated indirectly by the ATM protein kinase, transactivates target genes and thus induces cell cycle progression...

207

Low Dose Radiation Research Program: James E. Morris  

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Northwest National Laboratories Past Funded Project Sensitivity to radiation-induced cancer in hemachromatosis. Publications Stevens, R.G., Morris, J.E., and Anderson, L.E....

208

Low Dose Radiation Research Program: Walter E. Wilson  

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Monte Carlo simulation of the spatial distribution of energy deposition for an electron microbeam. Radiation Research: 163(4):468472. Mainardi, E., Donahue, R.J.,...

209

Low Dose Radiation Research Program: Howard L. Liber  

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Howard L. Liber Howard L. Liber Department of Environmental and Radiological Health Sciences Colorado State University Currently Funded Projects Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells Technical Abstracts 2003 Workshop: Delayed genomic instability in human lymphoblasts exposed to 137Cs y-rays radiation Schwartz, J.L., Jordan, R., Lenarczyk, M. and Liber, H.L. 2002 Workshop: Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells. Liber, H.L. and Schwartz, J.L. Publications Zhang, Y., Zhou, J., Held, K.D., Redmond, R.W., Prise, K.M., and Liber, H.L. (2008). Deficiencies of double-strand break repair factors and effects on mutagenesis in directly [gamma]-irradiated and medium-mediated bystander human lymphoblastoid cells. Radiation Research 169(2):197-206.

210

Low Dose Radiation Research Program: Gregory A. Kimmel  

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Gregory A. Kimmel Gregory A. Kimmel Pacific Northwest National Laboratory Past Project A Novel Spatially Resolved Cell Irradiator to Study Bystander and Adaptive Responses to Low-LET Radiation. Technical Abstracts 2002 Workshop: Spatially Resolved Single Cell Irradiator to Study Bystander Responses to Low LET Radiation. Resat, M.S., Kimmel, G.A., Miller, J.H., McDonald, J.C., Murphy, M.K., Strom, D.J., Thrall, B.D., and Colson, S.D. 2001 Workshop: Spatially Resolved Single Cell Irradiator to Study Bystander Responses to Low LET Radiation. Resat, M.S., Kimmel, G.A., Miller, J.H., McDonald, J.C., Murphy, M.K., Strom, D.J., Thrall, B.D., Metting, N.F., and Colson, S.D 1999 Workshop: A Novel, Spatially Resolved Cell Irradiator to Study Bystander and Adaptive Responses to Low-LET Radiation.

211

Low Dose Radiation Research Program: Mechanisms of Tissue Response...  

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whole body exposure to doses of 0.1 Gy to 5 Gy 60Co-g radiation in liver and mammary gland, which indicate that remodeling is a general and rapid consequence of irradiation but...

212

An integrated genetics approach to systemic low-dose radiation...  

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mammary tumor formation by combining our genetic diverse mouse model with the mammary gland radiation chimera model pioneered in the Barcellos-Hoff laboratory (3,4). As it takes...

213

Low Dose Radiation Research Program: Delayed Genomic Instability...  

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Lymphoblasts Exposed to 137Cs y-rays Radiation Authors: Jeffrey L. Schwartzb, Robert Jordan,b, Marek Lenarczyk,a and Howard L. Libera Institutions: a Department of Environmental...

214

Low Dose Radiation Research Program: Monte Carlo Track Structure...  

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Monte Carlo Track Structure Simulations for Low-LET Selected Cell Radiation Studies Walt Wilson Washington State University Tri-Cities Why This Project There are many types of...

215

Low Dose Radiation Research Program: Are Animals Heterozygous...  

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lymphocytes from animals of known genotypes. Radiation-induced foci occur in a time and dose dependent manner in the nuclei of normal cells, NBS cells on the other hand, do not...

216

Low Dose Radiation Research Program: The Characterization of...  

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acts as a tag for identification of the gene, by cloning the fusion mRNA and using RT-PCR techniques. We have conducted such a screen using moderate dose radiation (2 to 4 Gy,...

217

Early Brain Response to Low-Dose Radiation Exposure Involves Molecular Networks and Pathways Associated with Cognitive Functions, Advanced Aging and Alzheimer's Disease  

SciTech Connect

Understanding the cognitive and behavioral consequences of brain exposures to low-dose ionizing radiation has broad relevance for health risks from medical radiation diagnostic procedures, radiotherapy, environmental nuclear contamination, as well as earth orbit and space missions. Analyses of transcriptome profiles of murine brain tissue after whole-body radiation showed that low-dose exposures (10 cGy) induced genes not affected by high dose (2 Gy), and low-dose genes were associated with unique pathways and functions. The low-dose response had two major components: pathways that are consistently seen across tissues, and pathways that were brain tissue specific. Low-dose genes clustered into a saturated network (p < 10{sup -53}) containing mostly down-regulated genes involving ion channels, long-term potentiation and depression, vascular damage, etc. We identified 9 neural signaling pathways that showed a high degree of concordance in their transcriptional response in mouse brain tissue after low-dose radiation, in the aging human brain (unirradiated), and in brain tissue from patients with Alzheimer's disease. Mice exposed to high-dose radiation did not show these effects and associations. Our findings indicate that the molecular response of the mouse brain within a few hours after low-dose irradiation involves the down-regulation of neural pathways associated with cognitive dysfunctions that are also down regulated in normal human aging and Alzheimer's disease.

Lowe, Xiu R; Bhattacharya, Sanchita; Marchetti, Francesco; Wyrobek, Andrew J.

2008-06-06T23:59:59.000Z

218

Early Brain Response to Low-Dose Radiation Exposure Involves Molecular Networks and Pathways Associated with Cognitive Functions, Advanced Aging and Alzheimer's Disease  

SciTech Connect

Understanding the cognitive and behavioral consequences of brain exposures to low-dose ionizing radiation has broad relevance for health risks from medical radiation diagnostic procedures, radiotherapy, environmental nuclear contamination, as well as earth orbit and space missions. Analyses of transcriptome profiles of murine brain tissue after whole-body radiation showed that low-dose exposures (10 cGy) induced genes not affected by high dose (2 Gy), and low-dose genes were associated with unique pathways and functions. The low-dose response had two major components: pathways that are consistently seen across tissues, and pathways that were brain tissue specific. Low-dose genes clustered into a saturated network (p < 10{sup -53}) containing mostly down-regulated genes involving ion channels, long-term potentiation and depression, vascular damage, etc. We identified 9 neural signaling pathways that showed a high degree of concordance in their transcriptional response in mouse brain tissue after low-dose radiation, in the aging human brain (unirradiated), and in brain tissue from patients with Alzheimer's disease. Mice exposed to high-dose radiation did not show these effects and associations. Our findings indicate that the molecular response of the mouse brain within a few hours after low-dose irradiation involves the down-regulation of neural pathways associated with cognitive dysfunctions that are also down regulated in normal human aging and Alzheimer's disease.

Lowe, Xiu R; Bhattacharya, Sanchita; Marchetti, Francesco; Wyrobek, Andrew J.

2008-06-06T23:59:59.000Z

219

Low Dose Radiation Research Program: Modeling Intercellular Interactions  

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Modeling Intercellular Interactions During Radiation Carcinogenesis Modeling Intercellular Interactions During Radiation Carcinogenesis Authors: Rainer K Sachs,1 Michael Chan,2 Lynn Hlatky,3 Philip Hahnfeldt3 Institutions: 1Departments of Mathematics and Physics, University of California Berkeley California; 2School of Medicine, University of California at San Diego, La Jolla California; 3Department of Radiation Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston Massachusetts Abstract By modulating the microenvironment of malignant or pre-malignant epithelial cells, inhibitory or stimulatory signals from nearby cells, including those in stromal and vascular tissues, can play a key role in carcinogenesis; cancer is ultimately a disease of a whole-cell community, not just of a single cell, clone, or cell lineage. However, current commonly used

220

Low Dose Radiation Program: Links - Suggested Books and Literature for  

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Suggested Books and Literature for Teachers about Radiation Suggested Books and Literature for Teachers about Radiation Popular Press News Articles Newspapers is an excellent portal or gateway to newspapers from all 50 states. To find a newspaper of interest, select a state and click Search. For a more specified search, fill in the Title of the newspaper and click Search. The newspapers available from that state are listed alphabetically. Hall, E., Radiation and Life Hall, E.J.Radiobiology for the Radiologist. 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA. 2000 Nagai, T.Atomic Bomb Rescue and Relief Report. Nagasaki Association for Hibakushas' Medical Care (NASHIM), Nagasaki, Japan. 2000 Nagataki, S.Nagasaki Symposium on Chernobyl: Update and Future, Proceedings of "Chernobyl Update" 3 June 1994 at the 67th Annual Meeting of

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Low Dose Radiation Research Program: Interaction between Tissue and  

NLE Websites -- All DOE Office Websites (Extended Search)

between Tissue and Cellular Stress Responses: Effect of between Tissue and Cellular Stress Responses: Effect of TGF-ß Depletion on Radiation-Induced p53 Response M.H. Barcellos-Hoff, S.A. Ravani, R.L. Henshall, K.B. Ewan, R.L. Warters,* B. Parvin Lawrence Berkeley National Laboratory *University of Utah One of the most widely studied cellular responses to radiation is the activation of the transcription factor, p53, whose abundance and action dictates individual cellular fate decisions regarding proliferation, differentiation and death. A cell's response to damage needs to be rapid. Thus, it is not surprising that the activation of the p53 stress response primarily involves post-translational changes in the p53 protein. Whereas intracellular radiation-induced mediators of p53 stability have been the subject of intense study, little is known about the extracellular factors

222

Low Dose Radiation Research Program: Low-LET Microdosimetry Calculations  

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Low-LET Microdosimetry Calculations Low-LET Microdosimetry Calculations Authors: W.E. Wilson, J.H. Miller, D.J. Lynch, R.R. Lewis and M. Batdorf Institutions: Washington State University, Richland, WA, USA Liquid Model Calculations of low-linear-transfer (LET) microdosimetry have been extended to condensed phase by introducing new modules into the PITS code suite. Probability tables for inelastic interactions are constructed using the Dingfelder-GSF model for liquid-water cross-sections. Dingfelder et al. 1 re-evaluated low-energy electron interactions in liquid water in terms of five excitation and five ionization channels, and without assuming any collective interactions (plasmons). We use Dingfelder’s algorithms to calculate differential energy-loss distributions for the ten channels; by

223

Low Dose Radiation Research Program: Interaction of Genome and Cellular  

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of Genome and Cellular Micronenvioronment of Genome and Cellular Micronenvioronment Mina Bissell Life Sciences Division Lawrence Berkeley National Laboratory Why this Project While normal stoma can delay or prevent tumorigenesis, abnormal stromal components can promote tumor growth. Acquired or inherited mutations that alter stromal cell function can release the context-suppressed malignant cells. Literature spanning more than a century has shown that inflammation associated with tissue wounding can produce tunors. Radiation produces changes in reactive oxygen that are similar to inflammation and may represent a mechanism for radiation-induced damage. Project Goals To determine the underlying role of stromal alterations in controling genomic instability accompanying epithelial-mesenchyumal transformation.

224

Low Dose Radiation Research Program: Comparison of DNA Damage Risk from  

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Comparison of DNA Damage Risk from Low-Dose Radiation and Folate Comparison of DNA Damage Risk from Low-Dose Radiation and Folate Deficiency. Authors: Chantal Courtemanche, Arnold C. Huang, Nicole Kerry, Bernice Ng, and Bruce N. Ames. Institutions: Children’s Hospital Oakland Research Institute, Oakland, California. Our overall goal is to understand and quantify the real effects of low-dose radiation by measuring direct and specific cellular changes. However, since the background dose of radiation to which most individuals are exposed is well below the levels where significant biological effects, such as mutation or tumor induction, are observed, our novel approach is to compare the consequences of radiation to those of specific nutritional deficiencies. By determining which of these two common stresses at physiologically relevant doses leads to a greater amount of DNA damage, we

225

Ionizing Radiation Dosimetry  

Science Conference Proceedings (OSTI)

Ionizing Radiation Dosimetry. ... OH. US Air Force Radiation Dosimetry Laboratory, Wright-Patterson - Base, OH [100548- 0] PA. ...

2013-09-06T23:59:59.000Z

226

Low Dose Radiation Research Program: Molecular Mechanisms of  

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Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells Howard L. Liber Department of Environmental and Radiological Health Sciences Colorado State University Why This Project This research will be to investigate the condition known as genomic instability. This can be defined as a state in which genetic alterations, including chromosome aberrations and gene mutations, occur at rates that are much higher than normal. In fact, genomic instability is what allows a normal cell to accumulate the multiple genetic alterations that are required to convert it into a cancer cell. The chromosomes of human cells have structures at their ends called telomeres. Telomeres normally function to prevent chromosomes from fusing together end-to-end. An important

227

Low Dose Radiation Research Program: The Progeny of Irradiated Mammary  

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Progeny of Irradiated Mammary Epithelial Cells Exhibit a Phenotype Progeny of Irradiated Mammary Epithelial Cells Exhibit a Phenotype Characteristic of Malignancy Mary H. Barcellos-Hoff, R.L. Henshall-Powell, M.J. Bissell, and B. Parvin Lawrence Berkeley National Laboratory, Life Sciences Division We have proposed that the ability of radiation to induce altered microenvironments affects the frequency and features of neoplastic progression. Thus, we have sought to characterize the irradiated microenvironment and determine how these events contribute to mammary carcinogenesis. By using imaging bioinformatics to analyze mouse and human models of breast cancer we have now examined cell adhesion molecules (CAMs) critical for tissue-specific organization and function. We found that 1) radiation-induced microenvironments can contribute to neoplastic potential

228

Low Dose Radiation Research Program: Use of Computational Modeling to  

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Use of Computational Modeling to Evaluate Hypotheses about the Use of Computational Modeling to Evaluate Hypotheses about the Molecular and Cellular Mechanisms of Bystander Effects Authors: Yuchao “Maggie” Zhao and Rory Conolly Institutions: CIIT Centers for Health Research, 6 Davis Drive, Research Triangle Park, North Carolina A detailed understanding of the biological mechanisms of radiation-induced damage at the molecular and cellular levels is needed for accurate assessment of the shape of the dose-response curve for radiationinduced health effects in the intact organism. Computational models can contribute to the improved understanding of mechanisms through integration of data and quantitative evaluation of hypotheses. We propose to develop a novel computational model of bystander effects elicited by oxidative stress and a

229

Annexin A2 Regulates A Low Dose-Specific Stress Response To Radiation.  

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Annexin A2 Regulates A Low Dose-Specific Stress Response To Radiation. Thomas J. Annexin A2 Regulates A Low Dose-Specific Stress Response To Radiation. Thomas J. Weber (PI), Greg J. Newton, Ryan D. Quesenberry, Janani I. Shutthanandan, Nikki Bollinger, Heather E. Engelmann and Lee K. Opresko. Cell Biology and Biochemistry Group, Pacific Northwest National Laboratory, Richland, WA 99354. Previous studies have demonstrated that JB6 cells release cell transforming paracrine factors following exposure to a low dose of radiation (10 cGy). Investigation of secreted proteins by SDS-Page and silver stain led to the identification of a 36 kDa band whose levels were increased in medium from irradiated cells, relative to sham controls. The 36 kDa band was identified as annexin A2 by mass spectrometry. Western blot analysis confirmed a dose-dependent increase in annexin A2 levels associated with medium from

230

An integrated genetics approach to systemic low-dose radiation responses  

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integrated genetics approach to systemic low-dose radiation responses integrated genetics approach to systemic low-dose radiation responses G. Huang 1 , Y. Huang 1 , D.H. Nguyen 1 , K. Bjornstad 1 , C. Rosen 1 , P. Chang 2 , R. DelRosario 3 , Do Yup Lee 1 , B. Bowen 1 , W. Reindl 1 , J. Mott 1 , A. Balmain 3 , M.H. Barcellos-Hoff 4 , Joe W Gray 1,5 , Mina Bissell 1 , Gary Karpen 1 , T. Northen 1 , E. A. Blakely 1 , J. H. Mao 1 1 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 2 SRI International, Menlo Park, CA

231

Low Dose Radiation Research Program: Genetic Control of Repair and Adaptive  

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Repair and Adaptive Responses to Low-level DNA Damage Repair and Adaptive Responses to Low-level DNA Damage James E. Haber Brandeis University Why This Project In order to fully understand mechanisms resulting in effects of low dose, whole system rather than cells must be examined. Although not identical to mammalian systems, simple systems usually have many similarities and give direction for further study of more complex systems. We use the budding yeast, Saccharomyces cerevisiae, as a model system because it is easy to manipulate and its genome is simple and well characterized. Project Goals Examine mechanisms and effects of low dose radiation response for: Genetic recombination mechanisms that lead to genomic instability Genetic factors that affect individual susceptibility to low-dose radiation The adaptive response

232

Mechanisms of Low Dose Radiation-induced T helper Cell Function  

SciTech Connect

Exposure to radiation above levels normally encountered on Earth can occur during wartime, accidents such as those at Three Mile Island and Chernobyl, and detonation of dirty bombs by terrorists. Relatively high levels of radiation exposure can also occur in certain occupations (low-level waste sites, nuclear power plants, nuclear medicine facilities, airline industry, and space agencies). Depression or dysfunction of the highly radiosensitive cells of the immune system can lead to serious consequences, including increased risk for infections, cancer, hypersensitivity reactions, poor wound healing, and other pathologies. The focus of this research was on the T helper (Th) subset of lymphocytes that secrete cytokines (proteins), and thus control many actions and interactions of other cell types that make up what is collectively known as the immune system. The Department of Energy (DOE) Low Dose Radiation Program is concerned with mechanisms altered by exposure to high energy photons (x- and gamma-rays), protons and electrons. This study compared, for the first time, the low-dose effects of two of these radiation forms, photons and protons, on the response of Th cells, as well as other cell types with which they communicate. The research provided insights regarding gene expression patterns and capacity to secrete potent immunostimulatory and immunosuppressive cytokines, some of which are implicated in pathophysiological processes. Furthermore, the photon versus proton comparison was important not only to healthy individuals who may be exposed, but also to patients undergoing radiotherapy, since many medical centers in the United States, as well as worldwide, are now building proton accelerators. The overall hypothesis of this study was that whole-body exposure to low-dose photons (gamma-rays) will alter CD4+ Th cell function. We further proposed that exposure to low-dose proton radiation will induce a different pattern of gene and functional changes compared to photons. Over the course of this research, tissues other than spleens were archived and with funding obtained from other sources, including the Department of Radiation Medicine at the Loma Linda University Medical Center, some additional assays were performed. Furthermore, groups of additional mice were included that were pre-exposed to low-dose photons before irradiating with acute photons, protons, and simulated solar particle event (SPE) protons. Hence, the original support together with the additional funding for our research led to generation of much valuable information that was originally not anticipated. Some of the data has already resulted in published articles, manuscripts in review, and a number of presentations at scientific conferences and workshops. Difficulties in reliable and reproducible quantification of secreted cytokines using multi-plex technology delayed completion of this study for a period of time. However, final analyses of the remaining data are currently being performed and should result in additional publications and presentations in the near future. Some of the most notable conclusions, thus far, are briefly summarized below: - Distribution of leukocytes were dependent upon cell type, radiation quality, body compartment analyzed, and time after exposure. Low-dose protons tended to have less effect on numbers of major leukocyte populations and T cell subsets compared to low-dose photons. - The patterns of gene and cytokine expression in CD4+ T cells after protracted low-dose irradiation were significantly modified and highly dependent upon the total dose and time after exposure. - Patterns of gene and cytokine expression differed substantially among groups exposed to low-dose photons versus low-dose protons; differences were also noted among groups exposed to much higher doses of photons, protons, and simulated SPE protons. - Some measurements indicated that exposure to low-dose photon radiation, especially 0.01 Gy, significantly normalized at least some adverse effects of simulated SPE protons, thereby suggesting that this l

Gridley, Daila S.

2008-10-31T23:59:59.000Z

233

What can be learned from epidemiologic studies of persons exposed to low doses of radiation?  

SciTech Connect

The main objective of radiation risk assessment is to determine the risk of various adverse health effects associated with exposure to low doses and low dose rates. Extrapolation of risks from studies of persons exposed at high doses (generally exceeding 1 Sv) and dose rates has been the primary approach used to achieve this objective. The study of Japanese atomic bomb survivors in Hiroshima and Nagasaki has played an especially important role in risk assessment efforts. A direct assessment of the dose-response function based on studies of persons exposed at low doses and dose rates is obviously desirable. This paper focuses on the potential of both current and future nuclear workers studies for investigating the dose-response functions at low doses, and also discusses analyses making use of the low dose portion of the atomic bomb survivor data. Difficulties in using these data are the statistical imprecision of estimated dose-response parameters, and potential bias resulting from confounding factors and from uncertainties in dose estimates.

Gilbert, E.S.

1993-04-01T23:59:59.000Z

234

Low Dose Radiation Research Program: Using a Low LET Electron Microbeam to  

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Using a Low LET Electron Microbeam to Investigate Non-Targeted Using a Low LET Electron Microbeam to Investigate Non-Targeted Effects of Low Dose Radiation Authors: William F. Morgan1 and Marianne B. Sowa2 Institutions: 1Radiation Oncology Research Laboratory, University of Maryland, Baltimore, Maryland; 2Chemical Structure and Dynamics, Pacific Northwest National Laboratory, Richland Washington We have recently installed a low-linear energy transfer (LET) electron microbeam that generates energetic electrons to mimic radiation damage from gamma- and x-ray sources. It has been designed such that high-energy electrons deposit energy in a pre-selected subset of cells, leaving neighboring cells unirradiated (Figure 1). In this way it is possible to examine non-targeted effects associated with low dose radiation exposure,

235

Low Dose Radiation Research Program: Dual Regulation of JB6 Transformation  

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Dual Regulation of JB6 Transformation by Low Dose Gamma Radiation. Dual Regulation of JB6 Transformation by Low Dose Gamma Radiation. Authors: Thomas J. Weber,1 Lye M. Markillie,1 William B. Chrisler,1 Xingye C. Lei,1 and Nancy H. Colburn2 Institutions: 1Molecular Biosciences, Pacific Northwest National Laboratory. 2Gene Regulation Section, Basic Research Laboratory, National Cancer Institute JB6 mouse epidermal cells have been instrumental in defining the molecular mechanisms associated with neoplastic transformation in response to known tumor promoters. JB6 cells exhibit a clonal growth response to oxygen free radicals suggesting this model may also be useful for radiation research. Treatment of JB6 cells with 2 and 20 cGy gamma radiation resulted in a weak, but dose-dependent increase in anchorage-independent growth observed

236

Protection Against Ionizing Radiation in Extreme Radiation ...  

Science Conference Proceedings (OSTI)

Protection Against Ionizing Radiation in Extreme Radiation-resistant Microorganisms. ... Elucidated radiation protection by intracellular halides. ...

2013-05-01T23:59:59.000Z

237

A Low-Dose Ipsilateral Lung Restriction Improves 3-D Conformal Planning for Partial Breast Radiation Therapy  

Science Conference Proceedings (OSTI)

In trials of 3D conformal external beam partial breast radiotherapy (PBRT), the dosimetrist must balance the priorities of achieving high conformity to the target versus minimizing low-dose exposure to the normal structures. This study highlights the caveat that in the absence of a low-dose lung restriction, the use of relatively en-face fields may meet trial-defined requirements but expose the ipsilateral lung to unnecessary low-dose radiation. Adding a low-dose restriction that {dose resulted in successful plans in 88% of cases. This low-dose lung limit should be used in PBRT planning.

Mitchell, Tracy [Radiation Therapy Program, British Columbia Cancer Agency, Vancouver Island Centre, University of British Columbia, University of Victoria, Victoria, British Columbia (Canada); Truong, Pauline T., E-mail: ptruong@bccancer.bc.c [Radiation Therapy Program, British Columbia Cancer Agency, Vancouver Island Centre, University of British Columbia, University of Victoria, Victoria, British Columbia (Canada); Salter, Lee; Graham, Cathy; Gaffney, Helene; Beckham, Wayne; Olivotto, Ivo A. [Radiation Therapy Program, British Columbia Cancer Agency, Vancouver Island Centre, University of British Columbia, University of Victoria, Victoria, British Columbia (Canada)

2011-04-01T23:59:59.000Z

238

NVLAP Ionizing Radiation Dosimetry LAP  

Science Conference Proceedings (OSTI)

Ionizing Radiation Dosimetry LAP. ... This site has been established for applicants to the accreditation program for ionizing radiation dosimetry. ...

2013-07-23T23:59:59.000Z

239

NIST Ionizing Radiation Division - 2001  

Science Conference Proceedings (OSTI)

... The Ionizing Radiation Division of the Physics Laboratory supports the ... meaningful, and compatible measurements of ionizing radiations (x rays ...

240

CELLULAR RESPONSES AT LOW DOSES OF IONIZING RADIATION  

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and tissue levels including mutation induction and carcinogenesis. Furthermore, irradiation of the cytoplasm can initiate damage to the nucleus, and irradiated single cells...

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241

Low Dose Radiation Stimulates Antioxidant Capacity in the Brain and Lessens  

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Stimulates Antioxidant Capacity in the Brain and Lessens Stimulates Antioxidant Capacity in the Brain and Lessens Behavioral Symptoms in a 6-OHDA-Induced Rat Model of Parkinson's Disease Mohan Doss Fox Chase Cancer Center Abstract Background: Progressive degeneration of dopaminergic neurons in the substantia nigra (SN) pars compacta results in motor deficits in Parkinson’s disease (PD) patients. Oxidative damage to the nigral dopaminergic neurons has been implicated in the pathogenesis of Parkinson’s disease. Our hypothesis is that low dose radiation induces the production of antioxidants in the brain, which could provide protection to the dopaminergic neurons, potentially leading to prevention or stabilization of PD. The purpose of the study is (1) to determine the effect of low dose radiation on the total antioxidant capacity in SN in

242

Th Cell Gene Expression and Function in Response to Low Dose and Acute Radiation  

SciTech Connect

FINAL TECHNICAL REPORT Supported by the Low Dose Radiation Research Program, Office of Science U.S. Department of Energy Grant No. DE-FG02-07ER64345 Project ID: 0012965 Award Register#: ER64345 Project Manager: Noelle F. Metting, Sc.D. Phone: 301-903-8309 Division SC-23.2 noelle.metting@science.doe.gov Submitted March 2012 To: https://www.osti.gov/elink/241.3.jsp Title: Th Cell Gene Expression and Function in Response to Low Dose and Acute Radiation PI: Daila S. Gridley, Ph.D. Human low dose radiation data have been derived primarily from studies of space and airline flight personnel, nuclear plant workers and others exposed occupationally, as well as victims in the vicinity of atomic bomb explosions. The findings remain inconclusive due to population inconsistencies and complex interactions among total dose, dose rate, radiation quality and age at exposure. Thus, safe limits for low dose occupational irradiation are currently based on data obtained with doses far exceeding the levels expected for the general population and health risks have been largely extrapolated using the linear-nonthreshold dose-response model. The overall working hypothesis of the present study is that priming with low dose, low-linear energy transfer (LET) radiation can ameliorate the response to acute high-dose radiation exposure. We also propose that the efficacy of low-dose induced protection will be dependent upon the form and regimen of the high-dose exposure: photons versus protons versus simulated solar particle event protons (sSPE). The emphasis has been on gene expression and function of CD4+ T helper (Th) lymphocytes harvested from spleens of whole-body irradiated C57BL/6 mice, a strain that provides the genetic background for many genetically engineered strains. Evaluations of the responses of other selected cells, tissues such as skin, and organs such as lung, liver and brain were also initiated (partially funded by other sources). The long-term goal is to provide information that will be useful in estimating human health risks due to radiation that may occur during exposures in the work environment, nuclear/radiological catastrophes, as well as radiotherapy. Several papers have been published, accepted for publication or are in preparation. A number of poster and oral presentations have been made at scientific conferences and workshops. Archived tissues of various types will continue to be evaluated via funding from other sources (the DoE Low Dose Radiation Research Program, Office of Science and this specific grant will be appropriately included in the Acknowledgements of all subsequent publications/presentations). A post-doc and several students have participated in this study. More detailed description of the accomplishments is described in attached file.

Daila S. Gridley, PhD

2012-03-30T23:59:59.000Z

243

Low Dose Radiation Research Program: A Variable Energy Soft X-ray  

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Variable Energy Soft X-ray Microprobe to Investigate Mechanisms of Variable Energy Soft X-ray Microprobe to Investigate Mechanisms of the Radiation Induced Bystander Effect. Authors: Melvyn Folkard, Borivoj Vojnovic, Giuseppe Schettino, Kevin M Prise and Barry D Michael. Institutions: Gray Cancer Institute. We are currently engaged on two projects in the Low-dose Program: "Low dose studies with focused X-rays in cell and tissue models: mechanisms of bystander and genomic instability responses" (DE-FG07-99ER62877) and "Mechanistic modeling of bystander effects: An integrated theoretical and experimental approach" (DE-FG02-02ER63305). Central to both of these studies is a unique micro irradiation facility that uses ultrasoft X-rays focused to a sub micron beam for individual cell and sub cellular targeting. This facility allows us to selectively irradiate individual

244

Low Dose Radiation Research Program: A Variable Energy Soft X-ray  

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Variable Energy Soft X-ray Microprobe to Investigate Mechanisms of the Variable Energy Soft X-ray Microprobe to Investigate Mechanisms of the Radiation-Induced Bystander Effect Melvyn Folkard Gray Cancer Institute Why This Project The aim of this project is to determine the effects of low radiation doses using a machine that makes it possible to radiate one cell at a time. Our soft X-ray microprobe can irradiate individual cells, or locations within cells with defined doses and with sub-micron precision. We can use low doses approaching that of a single electron track, which is of relevance to environmental level exposures. Much of our work is concentrating on irradiating specified individual cells within cell populations to identify "bystander responses" where non-radiated cells respond to signals from nearby radiated cells. Higher energy x-rays are being generated to extend

245

An optimized colony forming assay for low-dose-radiation cell survival measurement  

Science Conference Proceedings (OSTI)

The aim of this study is to develop a simple and reliable method to quantify the cell survival of low-dose irradiations. Two crucial factors were considered, the same number of cells plated in each flask and an appropriate interval between cell plating and irradiation. For the former, we optimized cell harvest with trypsin, diluted cells in one container, and directly seeded cells on the bottom of flasks in a low density before irradiation. Reproducible plating efficiency was obtained. For the latter, we plated cells on the bottom of flasks and then monitored the processing of attachment, cell cycle variations, and the plating efficiency after exposure to 20 cGy of X-rays. The results showed that a period of 4.5 h to 7.5 h after plating was suitable for further treatment. In order to confirm the reliability and feasibility of our method, we also measured the survival curves of these M059K and M059J glioma cell lines by following the optimized protocol and obtained consistent results reported by others with cell sorting system. In conclusion, we successfully developed a reliable and simple way to measure the survival fractions of human cells exposed to low dose irradiation, which might be helpful for the studies on low-dose radiation biology.

Zhu J.; Sutherland B.; Hu W.; Ding N.; Ye C.; Usikalu M.; Li S.; Hu B.; Zhou G.

2011-11-01T23:59:59.000Z

246

Mechanisms underlying cellular responses of cells from haemopoeitic tissue to low dose-low LET radiation  

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underlying cellular responses of cells from haemopoeitic underlying cellular responses of cells from haemopoeitic tissue to low dose-low LET radiation Munira Kadhim 1 , Sarah Irons 1 , Deborah Bowler 1 , Virginia Serra 1 , Stefania Militi 2 , Kim Chapman 1 1 Genomic Instability Research Group, School of Life Sciences, Oxford Brookes University, Gipsy Lane, Headington, Oxford, Oxfordshire, OX3 0BP, UK 2 Mammalian Genetics Unit, Medical Research Council Harwell, Harwell Science and Innovation Campus, Oxfordshire, OX11 0RD, UK Radiation-induced responses at the cellular and whole body levels are influenced by genetic predisposition, with implications for environmental and potentially, diagnostic exposures. Currently, the extent to which genetic background play a role in the mechanisms and signalling pathways involved in radiation-induced

247

Low Dose Radiation Research Program: A Paracrine Signal Mediates The Cell  

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A Paracrine Signal Mediates The Cell Transformation Response To Low A Paracrine Signal Mediates The Cell Transformation Response To Low Dose Gamma Radiation in JB6 Cells. Authors: Thomas J. Weber,1 Robert W. Siegel,2 Lye M. Markillie,1 William B. Chrisler,1 Xingye C. Lei,3 and Nancy H. Colburn4 Institutions: 1Cell Biology and Biochemistry, Pacific Northwest National Laboratory, Richland, Washington. 2Protein Function, Pacific Northwest National Laboratory, Richland, Washington. 3Statistical and Mathematical Sciences, Pacific Northwest National Laboratory, Richland, Washington. 4Gene Regulation Section, Laboratory of Cancer Prevention, National Cancer Institute at Frederick, Frederick, Maryland. The carcinogenic response to radiation is complex and may involve adaptive cellular responses as well as a bystander effect mediated by paracrine or

248

Characterization of the role of Fhit in maintenance of genomic integrity following low dose radiation, in vivo and in vitro  

Science Conference Proceedings (OSTI)

The major goal of this study is to determine the effects of the Fhit pathway on low dose (radiation (IR)-induced genetic instability. Reduction of Fhit protein expression is observed in most solid tumors particularly in those tumors resulting from exposure to environmental carcinogens. Therefore, characterization of the role of the Fhit-dependent pathway in preventing low dose IR-induced genetic instability will provide useful parameters for evaluating the low dose IR-induced risk of mutagenesis and carcinogenesis. We pursued 3 specific aims to study our hypothesis that the Fhit-dependent pathways maintain genomic integrity through adjusting checkpoint response and repair genes expression following low dose IR. Aim 1: Determine whether Fhit interaction with RPA is necessary for Fhit to affect the cellular response to low dose IR. We combined the approaches of in vitro (GST pull-down and site-directed mutagenesis) and in vivo (observing the co-localization and immunoprecipitation of Fhit and RPA in Fhit knock out mouse cells transfected with mutant Fhit which has lost ability to interact with RPA in vitro). Aim 2: Determine the role of genes whose expression is affected by Fhit in low dose irradiated cells. We analyzed the distinct signature of gene expression in low dose irradiated Fhit-/- cells compared with Fhit+/+ cells by combining microarray, gene transfection and siRNA approaches. Aim 3: Determine the role of Fhit in genetic susceptibility to low dose IR in vivo. We compared the gene mutation frequency and the fragile site stability in the cells isolated from the Fhit+/+ and Fhit-/- mice at 1.5 years following low dose IR. These results determine the role of the Fhit-dependent pathway in maintaining genomic integrity in vitro and in vivo, which provide a basis for choosing surrogate markers in the Fhit-dependent pathway to evaluate low dose IR-induced risk of mutagenesis and carcinogenesis.

Wang, Ya

2010-05-14T23:59:59.000Z

249

NIST Ionizing Radiation Division - 1998  

Science Conference Proceedings (OSTI)

TECHNICAL ACTIVITIES 1998 - NISTIR 6268 IONIZING RADIATION DIVISION. The Neutron Interferometer. The neutron ...

250

NIST Ionizing Radiation Division - 2000  

Science Conference Proceedings (OSTI)

"Technical Activities 2000" - Table of Contents, Division home page. Ionizing Radiation Division. ...

251

Modular Systems Biology applied to TGFbeta and DNA Damage Response Signaling following Low Dose Radiation  

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Modular Systems Biology applied to TGFbeta and DNA Damage Response Signaling following Modular Systems Biology applied to TGFbeta and DNA Damage Response Signaling following Low Dose Radiation Francis A. Cucinotta 1 , Yongfeng Li 2 , Minli Wang 2 , Claudio Carra 2 , Janice Pluth 3 , and Peter O'Neill 4 1 NASA Johnson Space Center, Houston, TX 2 U.S.R.A. Division of Life Sciences, Houston TX 3 Lawrence Berkeley National Laboratory, Berkeley CA 4 Oxford University, Oxford UK Abstract: Modular systems biology (MSB) describes the complexity of biological systems using well defined modules that represent distinct biological response pathways or sub-systems within pathways. We review mathematical concepts from control theory that can be used to identify and construct well defined modules for describing complex biological processes. The DNA damage response and TGFbeta/Smad signaling are two important response pathways following

252

MOLECULAR MECHANISM OF SUPPRESSION OF NEOPLASTIC TRANSFORMATION BY LOW DOSES OF LOW LET RADIATION  

Science Conference Proceedings (OSTI)

We are currently funded (9/01-8/04) by the DOE Low Dose Radiation Research Program to examine mechanisms underlying the suppression of neoplastic transformation in vitro by low doses of low LET radiation. For the new studies proposed under Notice 04-21, we intend to follow up on our observation that upregulation of DNA repair may be an important factor and that its importance is dose-dependent. The experimental system will be the human hybrid cell neoplastic transformation assay that we are currently using. We propose to test the following hypothesis: Down-regulation of DNA dsb repair will abrogate the low dose suppression of neoplastic transformation. Using the technique of RNA silencing, it is proposed to test the effect of down-regulation of the two major DNA dsb repair pathways, homologous recombination (HR) and non-homologous end-joining (NHEJ), on the dose response relationship for neoplastic transformation. Based on prior studies, we predict that this will result in abrogation of the suppressive effect at doses in the range 1 to 10 cGy, but not at lower doses. The proposed experiments will also help address the question as to which of the two DNA repair pathways may be the most important in causing suppression of transformation. HR is a pathway that is predominant in S and G2 phase cells and is known to be less error-prone than the NHEJ pathway that is predominant in G1 phase. We hypothesize that down-regulation of HR will result in the most effective abrogation of suppression. An important component of this study will be the determination of the how abrogation of DNA dsb repair impacts the spontaneous transformation frequency, presumably a consequence of endogeneous DNA damage. Experiments will be carried out using partially synchronized populations of cells enriched for G1 and S/G2 respectively. In addition to the endpoint of neoplastic transformation the impact of down-regulation of HR and NHEJ on the formation and disappearance of the DNA dsb marker, gamma-H2AX, will be studied.

J.LESIE REDPATH, PH.D.

2011-03-29T23:59:59.000Z

253

Low dose radiation hypersensitivity and clustered DNA damages in human fibroblasts exposed to low dose and dose rate protons or 137CS y-rays  

SciTech Connect

Effective radioprotection for human space travelers hinges upon understanding the individual properties of charged particles. A significant fraction of particle radiation astronauts will encounter in space exploratory missions will come from high energy protons in galactic cosmic radiation (GCR) and/or possible exposures to lower energy proton flux from solar particle events (SPEs). These potential exposures present major concerns for NASA and others, in planning and executing long term space exploratory missions. We recently reported cell survival and transformation (acquisition of anchorage-independent growth in soft agar) frequencies in apparently normal NFF-28 primary human fibroblasts exposed to 0-30 cGy of 50MeV, 100MeV (SPE-like), or 1000 MeV (GCR-like) monoenergetic protons. These were modeled after 1989 SPE energies at an SPE-like low dose-rate (LDR) of 1.65 cGy/min or high dose rate (HDR) of 33.3 cGy/min delivered at the NASA Space Radiation Laboratory (NSRL) at BNL.

Bennett P. V.; Bennett, P.V.; Keszenman, D.J.; Johnson, A.M.; Sutherland, B.M.; Wilson, P.F.

2013-05-14T23:59:59.000Z

254

Prevention of Low Dose Radiation-Induced Genomic Instability with Clinically Relevant Non-Protein Thiols and Vitamin E.  

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Prevention of Low Dose Radiation-Induced Genomic Instability with Clinically Relevant Prevention of Low Dose Radiation-Induced Genomic Instability with Clinically Relevant Non-Protein Thiols and Vitamin E. J.S. Murley 1 , Y. Kataoka 1 , W.F. Morgan 2 , and D.J. Grdina 1 . The University of Chicago, Chicago, IL 1 , The University of Maryland Medical School, Baltimore, MD 2 Induced or delayed radioprotection is a novel phenomenon that shares many similarities with the low dose radiation-induced radiobiological phenomenon referred to as the adaptive response. Induced or delayed radioprotection is defined as an enhancement in the radiation resistance of cells at long times following their exposure to non-protein thiols (NPT) such as WR1065, the free thiol form of amifostine. This effect is the result of the induction of a cascade of intracellular

255

Effect of low-dose, low-LET γ-radiation and BaP injection on pulmonary  

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low-dose, low-LET γ-radiation and BaP injection on pulmonary low-dose, low-LET γ-radiation and BaP injection on pulmonary immunity in A/J mice K. Gott Lovelace Respiratory Research Institute Abstract Introduction: Low-dose, low-linear-energy-transfer (LET) radiation (LDR; < 100 mGy) activates the immune response (Nowosielska et al., 2006), presumably via epigenetic pathways (Scott et al., 2009) and has been implicated as suppressing both alpha-radiation-induced and smoking-related lung cancer (Scott et al. 2009). One of the hypothesized adaptive-response mechanisms by which LDR does so is by activating immune cell function in the lung, which would then increase their anti-cancer surveillance function (Liu, 2007; Bogdandi et al., 2010). One measure of activated immune cell function is their expression of markers on their cell surface that are

256

Chloroquine Improves Survival and Hematopoietic Recovery After Lethal Low-Dose-Rate Radiation  

SciTech Connect

Purpose: We have previously shown that the antimalarial agent chloroquine can abrogate the lethal cellular effects of low-dose-rate (LDR) radiation in vitro, most likely by activating the ataxia-telangiectasia mutated (ATM) protein. Here, we demonstrate that chloroquine treatment also protects against lethal doses of LDR radiation in vivo. Methods and Materials: C57BL/6 mice were irradiated with a total of 12.8 Gy delivered at 9.4 cGy/hour. ATM null mice from the same background were used to determine the influence of ATM. Chloroquine was administered by two intraperitoneal injections of 59.4 {mu}g per 17 g of body weight, 24 hours and 4 hours before irradiation. Bone marrow cells isolated from tibia, fibula, and vertebral bones were transplanted into lethally irradiated CD45 congenic recipient mice by retroorbital injection. Chimerism was assessed by flow cytometry. In vitro methylcellulose colony-forming assay of whole bone marrow cells and fluorescence activated cell sorting analysis of lineage depleted cells were used to assess the effect of chloroquine on progenitor cells. Results: Mice pretreated with chloroquine before radiation exhibited a significantly higher survival rate than did mice treated with radiation alone (80% vs. 31%, p = 0.0026). Chloroquine administration before radiation did not affect the survival of ATM null mice (p = 0.86). Chloroquine also had a significant effect on the early engraftment of bone marrow cells from the irradiated donor mice 6 weeks after transplantation (4.2% vs. 0.4%, p = 0.015). Conclusion: Chloroquine administration before radiation had a significant effect on the survival of normal but not ATM null mice, strongly suggesting that the in vivo effect, like the in vitro effect, is also ATM dependent. Chloroquine improved the early engraftment of bone marrow cells from LDR-irradiated mice, presumably by protecting the progenitor cells from radiation injury. Chloroquine thus could serve as a very useful drug for protection against the harmful effects of LDR radiation.

Lim Yiting [Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States)] [Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); Hedayati, Mohammad; Merchant, Akil A.; Zhang Yonggang; Yu, Hsiang-Hsuan M. [Department of Radiation Oncology and Molecular Radiation Sciences, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States)] [Department of Radiation Oncology and Molecular Radiation Sciences, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); Kastan, Michael B. [Department of Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee (United States) [Department of Oncology, St. Jude Children's Research Hospital, Memphis, Tennessee (United States); Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina (United States); Matsui, William, E-mail: matsuwi@jhmi.edu [Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States)] [Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States); DeWeese, Theodore L., E-mail: deweete@jhmi.edu [Department of Radiation Oncology and Molecular Radiation Sciences, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland (United States)

2012-11-01T23:59:59.000Z

257

Low Dose Radiation Research Program: Modeling the Physics of Damage Cluster  

NLE Websites -- All DOE Office Websites (Extended Search)

Modeling the Physics of Damage Cluster Formation in a Cellular Environment Modeling the Physics of Damage Cluster Formation in a Cellular Environment Larry Toburen East Carolina University Why This Project Modern tools of radiobiology are leading to many new discoveries regarding how cells and tissues respond to radiation exposure. We can now irradiate single cells and observe responses in adjacent cells. We can also measure clusters of radiation damage produced in DNA. The primary tools available to describe the initial spatial pattern of damage formed by the absorption of ionizing radiation are based on (MC) Monte Carlo simulations of the structure of charged particle tracks. Although many MC codes exist and considerable progress is being made in the incorporation of detailed macromolecular target structures into these codes, much of the interaction

258

Low dose radiation interations with the transformation growth factor (TGF)-beta pathway  

E-Print Network (OSTI)

A major limiting factor for long-term, deep-space missions is the radiation dose to astronauts. Because the dose to the astronauts is a mixed field of low- and high-LET radiation, there is a need to understand the effects of both radiation types on whole tissue; however, there are limited published data on the effects of high-LET (linearenergy- transfer) radiation on tissue. Thus, we designed a perfusion chamber system for rat trachea in order to mimic in vivo respiratory tissue. We successfully maintained the perfused tracheal tissue ex vivo in a healthy and viable condition for up to three days. In addition, this project studied the effects of high-LET Fe particles on the overall transformation growth factor (TGF)-beta response after TGF-beta inactivation and compared the results to the TGF-beta response post x-ray irradiation. It was found that a TGF-beta response could be measured in the perfused tracheal tissue, for x-ray and Fe particle irradiations, despite the high autofluorescent background intrinsic to tissue. However, after comparing the TGF-beta response of x-ray irradiation to High-Z-Highenergy (HZE) irradiation, there was not a significant difference in radiation types. The TGF-beta response in x-ray and HZE irradiated perfusion chambers was also measured over time post irradiation. It was found that for 6 hour and 8 hour post irradiation, the TGF-beta response was higher for lower doses of radiation than for higher doses. This is in contrast to the 0 hour fixation which found the TGF-beta response to increase with increased dose. The inverse relationship found for 6 hour and 8 hour fixation times may indicate a threshold response for TGF-beta response; i.e., for low doses, a threshold of dose must be reached for an immediate TGF-beta response, otherwise the tissue responds more slowly to the irradiation damage. This result was unexpected and will require further investigation to determine if the threshold can be determined for the 250 kVp x-rays and 1 Gev Fe particles.

Maslowski, Amy Jesse

2007-08-01T23:59:59.000Z

259

Regulation of Annexin A2 by Ionizing Radiation in Human Skin...  

NLE Websites -- All DOE Office Websites (Extended Search)

Annexin A2 by Ionizing Radiation in Human Skin Equivalent Culture: Does A Nuclear Annexin A2-Protein Kinase C Epsilon Complex Contribute To Reduced Cancer Risks At Low Dose...

260

Low Dose Radiation Induced DNA Damage Signaling and Repair Responses in  

NLE Websites -- All DOE Office Websites (Extended Search)

Induced DNA Damage Signaling and Repair Responses in Induced DNA Damage Signaling and Repair Responses in Human 3-Dimensional Skin Model System Yanrong Su, Jarah Meador and Adayabalam S. Balajee Center for Radiological Research, College of Physicians and Surgeons, Columbia University, 630 West, 168th Street, New York, NY 10032. Exposure to ionizing radiation (IR) inflicts a wide variety of lesions in the genomic DNA. Among them, DNA double strand break (DSB) is considered to be the critical lesion for most of the deleterious radiation effects including carcinogenesis. Much of our knowledge on induction and repair kinetics of DSB has come from studies in two dimensional cell culture systems. However, the damage signaling and repair responses to DSB in tissue microenvironment are largely unknown. Knowledge of tissue responses to

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they are not comprehensive nor are they the most current set.
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to obtain the most current and comprehensive results.


261

Low Dose Radiation Research Program: Kanokporn Noy Rithidech, Ph.D.  

NLE Websites -- All DOE Office Websites (Extended Search)

Kanokporn Noy Rithidech, Ph.D. Kanokporn Noy Rithidech, Ph.D. University of New York at Stony Brook Currently Funded Projects Induction of Genomic Instability In vivo by Low Doses of 137Cs Gamma Rays Technical Abstracts 2006 Workshop: No Evidence for In Vivo Induction of Genomic Instability in Bone Marrow Cells Collected from Mice Exposed to Low-Dose 137CS γ Rays Rithidech, K.N., Loetchutinat, C., Honikel, L., and Whorton, E.B. 2005 Workshop: Induction of Genomic Instability in Vivo by Low Doses of 137Cs γ rays. Rithidech, K.N., Leotchutinat, C., Honikel, L., Simon, S.R. and Whorton, E.B. 2003 Workshop: Induction of Genomic Instability in vivo by Low Doses of 137Cs y rays Rithidech. K., Whorton, E.B., Tungjai, M., Ar-Bab, E., Simon, S.R. Tawde, M. and Anderson, C.W. 2002 Workshop: Induction of Genomic Instability In vivo by Low Doses of 137Cs gamma rays.

262

Ionizing radiation detector  

DOE Patents (OSTI)

An ionizing radiation detector is provided which is based on the principal of analog electronic integration of radiation sensor currents in the sub-pico to nano ampere range between fixed voltage switching thresholds with automatic voltage reversal each time the appropriate threshold is reached. The thresholds are provided by a first NAND gate Schmitt trigger which is coupled with a second NAND gate Schmitt trigger operating in an alternate switching state from the first gate to turn either a visible or audible indicating device on and off in response to the gate switching rate which is indicative of the level of radiation being sensed. The detector can be configured as a small, personal radiation dosimeter which is simple to operate and responsive over a dynamic range of at least 0.01 to 1000 R/hr.

Thacker, L.H.

1989-06-08T23:59:59.000Z

263

Ionizing radiation detector  

DOE Patents (OSTI)

An ionizing radiation detector is provided which is based on the principle of analog electronic integration of radiation sensor currents in the sub-pico to nano ampere range between fixed voltage switching thresholds with automatic voltage reversal each time the appropriate threshold is reached. The thresholds are provided by a first NAND gate Schmitt trigger which is coupled with a second NAND gate Schmitt trigger operating in an alternate switching state from the first gate to turn either a visible or audible indicating device on and off in response to the gate switching rate which is indicative of the level of radiation being sensed. The detector can be configured as a small, personal radiation dosimeter which is simple to operate and responsive over a dynamic range of at least 0.01 to 1000 R/hr.

Thacker, Louis H. (Knoxville, TN)

1990-01-01T23:59:59.000Z

264

Low Dose Radiation Research Program: Multi-cellular Crosstalk in Radiation  

NLE Websites -- All DOE Office Websites (Extended Search)

About this Project About this Project Multi-cellular Crosstalk in Radiation Damage Technical Abstracts 2006 Workshop: Low-LET Bystander Effects in Cells In Vitro Are Significantly Less Than Published For High-LET Radiation Blakely, E.A., Thompson, A.C., Chang, P., Schwarz, R.I., Bjornstad, K., Rosen, C., Wisnewski, C., and Mocherla, D. 2005 Workshop: X-ray Microbeam Bystander Studies with Human Mammary Epithelial Cells and Fibroblasts Blakely, E.A., Schwarz, R.I., Thompson, A.C., Bjornstad, K.A., Chang, P.Y., Rosen, C.J., Sudar, D., Romano, R., and Parvin, B. 2003 Workshop: 12.5 keV X-ray Microbeam Bystander Studies with Human Mammary Epithelial Cells and Fibroblasts Blakely, E.A., Schwarz, R.I., Thompson, A.C., Bjornstad, K.A., Chang, P.Y., Rosen, C.J., and Sudar, D. 2001 Workshop:

265

Low Dose Radiation Response Curves, Networks and Pathways in Human Lymphoblastoid Cells Exposed from 1 to 10 cGy of Acute Gamma Radiation  

E-Print Network (OSTI)

R.B. Mikkelsen, Ionizing radiation-induced, mitochondria-W.K. Rorrer, P.B. Chen, Radiation-induced proliferation ofresponse genes to ionizing radiation in human lymphoblastoid

Wyrobek, A. J.

2011-01-01T23:59:59.000Z

266

Adaptive Responses to Low Dose/Low Dose-Rate γ-Rays in Normal Human Fibroblasts:  

NLE Websites -- All DOE Office Websites (Extended Search)

Responses to Low Dose/Low Dose-Rate γ-Rays in Normal Human Fibroblasts: Responses to Low Dose/Low Dose-Rate γ-Rays in Normal Human Fibroblasts: The Role of Oxidative Metabolism Edouard I. Azzam 1 , Sonia M. de Toledo 1 , Badri N. Pandey 1 , Perumal Venkatachalam 1 , Manuela Buoannano 1 , Zhi Yang 1 , Ling Li 3 , Donna M. Gordon 2 , Roger W. Howell 1 , Debkumar Pain 2 and Douglas R. Spitz 3 1 Department of Radiology, 2 Department of Pharmacology and Physiology, UMDNJ-New Jersey Medical School, Newark, NJ 3 Free Radical and Radiation Biology Program, University of Iowa, Iowa City, IA To investigate low dose/low dose-rate effects of low linear energy transfer ionizing radiation, we used γ-irradiated cells adapted to grow in three-dimensional architecture that mimics cell growth in vivo. We determined cellular, molecular and biochemical changes in these

267

NIST Ionizing Radiation Division 1999 - Future Directions  

Science Conference Proceedings (OSTI)

... to test the applicability of EPR tooth enamel retrospective dosimetry to dose assessment of background radiation. The low-dose threshold (dose ...

268

Ionizing Radiation Division Quality Manual  

Science Conference Proceedings (OSTI)

... 08 47020C Low-energy Photon Brachytherapy Seeds, ... Calibrated for Surface Dose Rate 10 ... Sources Calibrated for Radiation Protection Ionization ...

2012-03-08T23:59:59.000Z

269

Low Dose Radiation Response Curves, Networks and Pathways in Human Lymphoblastoid Cells Exposed from 1 to 10 cGy of Acute Gamma Radiation  

Science Conference Proceedings (OSTI)

We investigated the low dose dependency of the transcriptional response of human cells to characterize the shape and biological functions associated with the dose response curve and to identify common and conserved functions of low dose expressed genes across cells and tissues. Human lymphoblastoid (HL) cells from two unrelated individuals were exposed to graded doses of radiation spanning the range of 1-10 cGy were analyzed by transcriptome profiling, qPCR and bioinformatics, in comparison to sham irradiated samples. A set of {approx}80 genes showed consistent responses in both cell lines; these genes were associated with homeostasis mechanisms (e.g., membrane signaling, molecule transport), subcellular locations (e.g., Golgi, and endoplasmic reticulum), and involved diverse signal transduction pathways. The majority of radiation-modulated genes had plateau-like responses across 1-10 cGy, some with suggestive evidence that transcription was modulated at doses below 1 cGy. MYC, FOS and TP53 were the major network nodes of the low-dose response in HL cells. Comparison our low dose expression findings in HL cells with those of prior studies in mouse brain after whole body exposure, in human keratinocyte cultures, and in endothelial cells cultures, indicates that certain components of the low dose radiation response are broadly conserved across cell types and tissues, independent of proliferation status.

Wyrobek, A. J.; Manohar, C. F.; Nelson, D. O.; Furtado, M. R.; Bhattacharya, M. S.; Marchetti, F.; Coleman, M.A.

2011-04-18T23:59:59.000Z

270

Genetic profiling of lymphoblastoid cell lines sensitive to low dose  

NLE Websites -- All DOE Office Websites (Extended Search)

profiling of lymphoblastoid cell lines sensitive to low dose profiling of lymphoblastoid cell lines sensitive to low dose radiation David Rocke University of California Davis Abstract Previous study from our laboratory has identified pathways associated with low dose ionizing radiation (LDIR) in vivo that is consistent across individuals. Furthermore, gene expression patterns have revealed genetic variation between individuals, which may play a role in individual sensitivity to LDIR. The aim is to evaluate microRNA and mRNA expression patterns in lymphoblast cell lines that exhibit sensitivity to radiation. Human lymphoblastoid cell lines were screened for low dose radiation sensitivity by apoptosis, cellular proliferation, and colony forming assay. Cells were irradiated with 5cGy and 10cGy and analyzed at multiple time

271

Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells  

Science Conference Proceedings (OSTI)

A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

Jian Li

2012-11-07T23:59:59.000Z

272

Low Dose Radiation Research Program: 12.5 keV Xray Microbeam Bystander  

NLE Websites -- All DOE Office Websites (Extended Search)

12.5 keV Xray Microbeam Bystander Studies With Human Mammary 12.5 keV Xray Microbeam Bystander Studies With Human Mammary Epithelial Cells and Fibroblasts Authors: E. A. Blakely1, R. I. Schwarz1, A. C. Thompson2, K. A. Bjornstad1, P. Y. Chang1,3 C.J. Rosen1, and D. Sudar1 Institutions: Divisions of 1Life Sciences and 2Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA. and 3SRI International, Menlo Park, CA. We are using a novel x-ray Microprobe Beamline at the Advanced Light Source (ALS) at LBNL to investigate bystander effects of low doses in well characterized human mammary epithelial cells (HMEC) and human skin fibroblasts (HSF). The ALS facility is capable of producing a beam of 12.5 keV x-rays with a focussed spot size of __m_ and a wide range of doses and dose-rates. Unlike normal x-ray sources, this beam has a very small background of either low-

273

NFkB-mediated Prosurvival Network in Low Dose Radiation-induced...  

NLE Websites -- All DOE Office Websites (Extended Search)

approaches to improve normal tissue protection in caner radiation therapy. We found that ATM, a DNA damage sensor, is induced by LDR and responsible for activation of transcription...

274

Advanced Computational Approaches for Characterizing Stochastic Cellular Responses to Low Dose, Low Dose Rate Exposures  

SciTech Connect

OAK - B135 This project final report summarizes modeling research conducted in the U.S. Department of Energy (DOE), Low Dose Radiation Research Program at the Lovelace Respiratory Research Institute from October 1998 through June 2003. The modeling research described involves critically evaluating the validity of the linear nonthreshold (LNT) risk model as it relates to stochastic effects induced in cells by low doses of ionizing radiation and genotoxic chemicals. The LNT model plays a central role in low-dose risk assessment for humans. With the LNT model, any radiation (or genotoxic chemical) exposure is assumed to increase ones risk of cancer. Based on the LNT model, others have predicted tens of thousands of cancer deaths related to environmental exposure to radioactive material from nuclear accidents (e.g., Chernobyl) and fallout from nuclear weapons testing. Our research has focused on developing biologically based models that explain the shape of dose-response curves for low-dose radiation and genotoxic chemical-induced stochastic effects in cells. Understanding the shape of the dose-response curve for radiation and genotoxic chemical-induced stochastic effects in cells helps to better understand the shape of the dose-response curve for cancer induction in humans. We have used a modeling approach that facilitated model revisions over time, allowing for timely incorporation of new knowledge gained related to the biological basis for low-dose-induced stochastic effects in cells. Both deleterious (e.g., genomic instability, mutations, and neoplastic transformation) and protective (e.g., DNA repair and apoptosis) effects have been included in our modeling. Our most advanced model, NEOTRANS2, involves differing levels of genomic instability. Persistent genomic instability is presumed to be associated with nonspecific, nonlethal mutations and to increase both the risk for neoplastic transformation and for cancer occurrence. Our research results, based on applications of NEOTRANS2, indicate that nonlinear threshold-type, dose-response relationships for excess stochastic effects (problematic nonlethal mutations, neoplastic transformation) should be expected after exposure to low linear energy transfer (LET) gamma rays or gamma rays in combination with high-LET alpha radiation. Similar thresholds are expected for low-dose-rate low-LET beta irradiation. We attribute the thresholds to low-dose, low-LET radiation induced protection against spontaneous mutations and neoplastic transformations. The protection is presumed mainly to involve selective elimination of problematic cells via apoptosis. Low-dose, low-LET radiation is presumed to trigger wide-area cell signaling, which in turn leads to problematic bystander cells (e.g., mutants, neoplastically transformed cells) selectively undergoing apoptosis. Thus, this protective bystander effect leads to selective elimination of problematic cells (a tissue cleansing process in vivo). However, this protective bystander effects is a different process from low-dose stimulation of the immune system. Low-dose, low-LET radiation stimulation of the immune system may explain why thresholds for inducing excess cancer appear much larger (possibly more than 100-fold larger) than thresholds for inducing excess mutations and neoplastic transformations, when the dose rate is low. For ionizing radiation, the current risk assessment paradigm is such that the relative risk (RR) is always 1, no matter how small the dose. Our research results indicate that for low-dose or low-dose-rate, low-LET irradiation, RR < 1 may be more the rule than the exception. Directly tied to the current RR paradigm are the billion-dollar cleanup costs for radionuclide-contaminated DOE sites. Our research results suggest that continued use of the current RR paradigm for which RR 1 could cause more harm than benefit to society (e.g., by spreading unwarranted fear about phantom excess risks associated with low-dose low-LET radiation). Such phantom risks also may arise from risk assessments conducted for com

Scott, Bobby, R., Ph.D.

2003-06-27T23:59:59.000Z

275

Advanced Computational Approaches for Characterizing Stochastic Cellular Responses to Low Dose, Low Dose Rate Exposures  

Science Conference Proceedings (OSTI)

OAK - B135 This project final report summarizes modeling research conducted in the U.S. Department of Energy (DOE), Low Dose Radiation Research Program at the Lovelace Respiratory Research Institute from October 1998 through June 2003. The modeling research described involves critically evaluating the validity of the linear nonthreshold (LNT) risk model as it relates to stochastic effects induced in cells by low doses of ionizing radiation and genotoxic chemicals. The LNT model plays a central role in low-dose risk assessment for humans. With the LNT model, any radiation (or genotoxic chemical) exposure is assumed to increase ones risk of cancer. Based on the LNT model, others have predicted tens of thousands of cancer deaths related to environmental exposure to radioactive material from nuclear accidents (e.g., Chernobyl) and fallout from nuclear weapons testing. Our research has focused on developing biologically based models that explain the shape of dose-response curves for low-dose radiation and genotoxic chemical-induced stochastic effects in cells. Understanding the shape of the dose-response curve for radiation and genotoxic chemical-induced stochastic effects in cells helps to better understand the shape of the dose-response curve for cancer induction in humans. We have used a modeling approach that facilitated model revisions over time, allowing for timely incorporation of new knowledge gained related to the biological basis for low-dose-induced stochastic effects in cells. Both deleterious (e.g., genomic instability, mutations, and neoplastic transformation) and protective (e.g., DNA repair and apoptosis) effects have been included in our modeling. Our most advanced model, NEOTRANS2, involves differing levels of genomic instability. Persistent genomic instability is presumed to be associated with nonspecific, nonlethal mutations and to increase both the risk for neoplastic transformation and for cancer occurrence. Our research results, based on applications of NEOTRANS2, indicate that nonlinear threshold-type, dose-response relationships for excess stochastic effects (problematic nonlethal mutations, neoplastic transformation) should be expected after exposure to low linear energy transfer (LET) gamma rays or gamma rays in combination with high-LET alpha radiation. Similar thresholds are expected for low-dose-rate low-LET beta irradiation. We attribute the thresholds to low-dose, low-LET radiation induced protection against spontaneous mutations and neoplastic transformations. The protection is presumed mainly to involve selective elimination of problematic cells via apoptosis. Low-dose, low-LET radiation is presumed to trigger wide-area cell signaling, which in turn leads to problematic bystander cells (e.g., mutants, neoplastically transformed cells) selectively undergoing apoptosis. Thus, this protective bystander effect leads to selective elimination of problematic cells (a tissue cleansing process in vivo). However, this protective bystander effects is a different process from low-dose stimulation of the immune system. Low-dose, low-LET radiation stimulation of the immune system may explain why thresholds for inducing excess cancer appear much larger (possibly more than 100-fold larger) than thresholds for inducing excess mutations and neoplastic transformations, when the dose rate is low. For ionizing radiation, the current risk assessment paradigm is such that the relative risk (RR) is always 1, no matter how small the dose. Our research results indicate that for low-dose or low-dose-rate, low-LET irradiation, RR fear about phantom excess risks associated with low-dose low-LET radiation). Such phantom risks also may arise from risk assessments conducted for com

Scott, Bobby, R., Ph.D.

2003-06-27T23:59:59.000Z

276

The Circadian Rhythm, A Continuous Transcription-Translation Feedback Loop, Contributes to Low-Dose Radiation-Induced Radioadaptive Response  

NLE Websites -- All DOE Office Websites (Extended Search)

The Circadian Rhythm, A Continuous Transcription-Translation Feedback Loop, Contributes to Low-Dose Radiation-Induced Radioadaptive Response Aris Alexandrou and Jian Jian Li Department of Radiation Oncology, the University of California Davis, Sacramento, California, 95817 The initiation of the circadian rhythm field occurred when the Takahashi group defined a mutation in the mouse gene "Clock" and cloned the circadian locomotor output cycles kaput (Clock) in the mid- 1990's (1-3). Currently more than a dozen clock genes have been identified (3-4). Disruptions in the circadian rhythm via changes in environmental conditions, such as, diet, temperature, and night/day hours lead to the pathogenesis of a multitude of diseases, such as, cancer, diabetes mellitus,

277

Ionizing Radiation Injury (South Carolina)  

Energy.gov (U.S. Department of Energy (DOE))

This legislation applies to employers that have more than one employee who engages in activities which involve the presence of ionizing radiation. Employers with less than three employees can...

278

Low Dose Radiation Research Program: A Variable-Energy Soft X-Ray  

NLE Websites -- All DOE Office Websites (Extended Search)

A Variable-Energy Soft X-Ray Microprobe to Investigate Mechanisms of A Variable-Energy Soft X-Ray Microprobe to Investigate Mechanisms of the Radiation-Induced Bystander Effect. Authors: Melvyn Folkard, Borivoj Vojnovic, Giuseppe Schettino, Kirk Atkinson, Kevin M Prise, Barry D Michael Institutions: Gray Cancer Institute, PO BO Box100, Mount Vernon Hospital, Northwood, HA6 2JR, UK The Gray Cancer Institute (GCI) has pioneered the use of X-ray focussing techniques to develop systems for micro-irradiating individual cells and sub-cellular targets. Our prototype X-ray microprobe was developed alongside our existing charged-particle microbeam to address problems specific to low LET radiations, or where very precise targeting accuracy and dose delivery are required. This facility was optimised for focusing 278 eV CK X-rays; however there are a number of reasons for extending the

279

Radiation Leukemogenesis: Applying Basic Science of Epidemiological Estimates of Low Dose Risks and Dose-Rate Effects  

SciTech Connect

The next stage of work has been to examine more closely the A-bomb leukemia data which provides the underpinnings of the risk estimation of CML in the above mentioned manuscript. The paper by Hoel and Li (Health Physics 75:241-50) shows how the linear-quadratic model has basic non-linearities at the low dose region for the leukemias including CML. Pierce et. al., (Radiation Research 123:275-84) have developed distributions for the uncertainty in the estimated exposures of the A-bomb cohort. Kellerer, et. al., (Radiation and Environmental Biophysics 36:73-83) has further considered possible errors in the estimated neutron values and with changing RBE values with dose and has hypothesized that the tumor response due to gamma may not be linear. We have incorporated his neutron model and have constricted new A-bomb doses based on his model adjustments. The Hoel and Li dose response analysis has also been applied using the Kellerer neutron dose adjustments for the leukemias. Finally, both Pierce's dose uncertainties and Kellerer neutron adjustments are combined as well as the varying RBE with dose as suggested by Rossi and Zaider and used for leukemia dose-response analysis. First the results of Hoel and Li showing a significantly improved fit of the linear-quadratic dose response by the inclusion of a threshold (i.e. low-dose nonlinearity) persisted. This work has been complete for both solid tumor as well as leukemia for both mortality as well as incidence data. The results are given in the manuscript described below which has been submitted to Health Physics.

Hoel, D. G.

1998-11-01T23:59:59.000Z

280

Lesson 4 - Ionizing Radiation | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

4 - Ionizing Radiation 4 - Ionizing Radiation Lesson 4 - Ionizing Radiation Lesson Three showed that unstable isotopes emit energy as they become more stable. This energy is known as radiation. This lesson explores forms of radiation, where radiation is found, how we detect and measure radiation, what sources of radiation people are exposed to, whether radiation is harmful, and how we can limit our exposure. Specific topics covered in this lesson include: Types of radiation Non-ionizing Ionizing Forms of ionizing radiation Alpha particles Beta particles Gamma rays Radiation Decay chain Half-life Dose Radiation measurements Sources of radiation Average annual exposure Lesson 4 - Ionizing Radiation.pptx More Documents & Publications DOE-HDBK-1130-2008 DOE-HDBK-1130-2008 DOE-HDBK-1130-2007

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Effect of low-dose, low-LET γ-radiation and BaP injection on pulmonary immunity in A/J mice  

NLE Websites -- All DOE Office Websites (Extended Search)

low-dose, low-LET γ-radiation and BaP injection on pulmonary immunity in A/J mice low-dose, low-LET γ-radiation and BaP injection on pulmonary immunity in A/J mice K. Gott, V. Gonzales, M. Makvandi, N. Kikendall, A. Monier, E. Maloy, C. Rietz, B. Scott and J. Wilder. Lovelace Respiratory Research Institute, Albuquerque, NM Introduction: Low-dose, low-linear-energy-transfer (LET) radiation (LDR; < 100 mGy) activates the immune response (Nowosielska et al., 2006), presumably via epigenetic pathways (Scott et al., 2009) and has been implicated as suppressing both alpha-radiation-induced and smoking-related lung cancer (Scott et al. 2009). One of the hypothesized adaptive-response mechanisms by which LDR does so is by activating immune cell function in the lung, which would then increase their anti-cancer surveillance

282

Low Dose Radiation Research Program: A Variable-Energy Soft X-Ray  

NLE Websites -- All DOE Office Websites (Extended Search)

A Variable-Energy Soft X-Ray Microprobe to Investigate Mechanisms of A Variable-Energy Soft X-Ray Microprobe to Investigate Mechanisms of the Radiation -Induced Bystander Effect. Authors: Melvyn Folkard, Borivoj Vojnovic, Giuseppe Schettino, Kirk Atkinson, Kevin M Prise, Barry D Michael Institutes: Gray Cancer Institute, PO Box 100, Mount Vernon Hospital, Northwood, HA6 2JR, UK For over a decade, the Gray Cancer Institute (GCI) has been actively engaged in the development and use of micro-irradiation techniques applied to radiobiological research. Our initial investigations made use of a charged-particle microbeam capable of irradiating individual cells with collimated energetic protons or 3He ions. By the end of the 1990's, a second facility had been constructed, which uses diffractive X-ray optics to focus ultrasoft X-rays to a sub-micron spot. The X-ray microprobe was

283

Low dose radiation effects of multipotent neural stem and progenitor cells  

NLE Websites -- All DOE Office Websites (Extended Search)

effects of multipotent neural stem and progenitor cells effects of multipotent neural stem and progenitor cells Charles L. Limoli, Department of Radiation Oncology, University of California, Irvine 92697-2695 Multipotent neural cells (both stem cells and their precursor cell progeny) retain their capacity to proliferate and differentiate throughout the mammalian lifespan. High numbers of these cells are located within the dentate subgranular zone (SGZ) of the hippocampus and the subventricular (SVZ) zone adjacent to the lateral ventricles, where they produce cells that can migrate away and differentiate into neurons (neurogenesis) and glia (gliogenesis). The realization that the brain contains such cells has sparked intense interest and speculation regarding their potential function. While significant data

284

Low Dose Radiation Research Program: Real-time Study of Signal Transduction  

NLE Websites -- All DOE Office Websites (Extended Search)

Real-time Study of Signal Transduction Pathways Involving in Real-time Study of Signal Transduction Pathways Involving in Bystander Effects Using Single Nanoparticle Optics and Single Living Cell Imaging Authors: Prakash D. Nallathamby, X. Nancy Xu, Mohan Natarajan Institutions: Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, Virginia and Department of Radiation Oncology, The University of Texas Health Science Center, San Antonio, Texas The mechanisms of bystander effects remain largely unknown. Bystander responses are thought to depend on activation of cellular communication processes. Recent studies have speculated that several crucial signal transduction pathways could play a major role in bystander effects. These crucial signal transduction pathways are controlled by a coordinated

285

Community Surveys: Low Dose Radiation. Fernald, Ohio and Rocky Flats, Colorado  

SciTech Connect

This report is intended to present a basic description of the data from the two community surveys and to document the text of the questions; the methods used for the survey data collection; and a brief overview of the results. Completed surveys were conducted at local communities near the Rocky Flats, Colorado and the Fernald, Ohio sites; no survey was conducted for the Brookhaven, New York site. Fernald. The Fernald sample was randomly selected from 98% of all potential residential telephones in the townships of Ross, Morgan, and Crosby. The only telephone exchanges not used for the Fernald study had 4%, or fewer, of the holders of the telephone numbers actually living in either of the three target townships. Surveying started on July 24, 2001 and finished on August 30, 2001. A total of 399 completed interviews were obtained resulting in a CASRO response rate of 41.8%. The average length of an interview was 16.5 minutes. Rocky Flats. The sample was randomly selected from all potential residential telephones in Arvada and from 99% of the potential telephones in Westminster. Surveying started on August 10, 2001 and finished on September 25, 2001. A total of 401 completed interviews were obtained with a CASRO response rate of 32.5%. The average length of an interview was 15.7 minutes. Overall, respondents hold favorable views of science. They indicate an interest in developments in science and technology, feel that the world is better off because of science, and that science makes our lives healthier, easier, and more comfortable. However, respondents are divided on whether science should decide what is safe or not safe for themselves and their families. The majority of the respondents think that standards for exposure to radiation should be based on what science knows about health effects of radiation and on what is possible with today's technology. Although few respondents had visited the sites, most had heard or read something about Fernald or Rocky Flat s in the media. Impressions of the sites tend to be negative. Most respondents feel that overall their community would be better off without the site. However, when asked about the economic future of their community after cleanup and closure of the site, only 31-43% thought that it will be better, 47-56% thought their local economy will be about the same.

C. K. Mertz; James Flynn; Donald G. MacGregor; Theresa Satterfield; Stephen M. Johnson; Seth Tuler; Thomas Webler

2002-10-16T23:59:59.000Z

286

Chromatin Regulation and Low Dose Irradiation: A Two-Way Street ?  

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Chromatin Regulation and Low Dose Irradiation: A Two-Way Street ? Chromatin Regulation and Low Dose Irradiation: A Two-Way Street ? Irene Chiolo, Sylvain Costes and Gary H. Karpen. Lawrence Berkeley National Lab and UC Berkeley, Berkeley, CA Goal: We are trying to understand the impact of exposure to low-dose ionizing radiation on epigenetic mechanisms, chromatin organization and functional responses, and how these processes affect the response to low-dose radiation. Background/Significance: Genomic instability (GI) is one of the hallmarks of cancer that contributes to genetic diversity and has been associated with exposure to ionizing radiation (IR) (1,2). Radiobiology dogma has focused on DNA as the likely target for GI effects of radiation, but until fairly recently, without incorporating roles for chromatin.

287

Chromatin Regulation and Low Dose Irradiation: A Two-Way Street?  

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Chromatin Regulation and Low Dose Irradiation: A Two-Way Street? Chromatin Regulation and Low Dose Irradiation: A Two-Way Street? Irene Chiolo Lawrence Berkeley National Lab Abstract Goal: We are trying to understand the impact of exposure to low-dose ionizing radiation on epigenetic mechanisms, chromatin organization and functional responses, and how these processes affect the response to low-dose radiation. Background/Significance: Genomic instability (GI) is one of the hallmarks of cancer that contributes to genetic diversity and has been associated with exposure to ionizing radiation (IR) (1,2). Radiobiology dogma has focused on DNA as the likely target for GI effects of radiation, but until fairly recently, without incorporating roles for chromatin. Eukaryotic genomes contain two major chromatin domains: heterochromatin and

288

Latest Research, Response to Fukushima, Integrating Low Dose...  

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Latest Research, Response to Fukushima, Integrating Low Dose into Policy-All Featured at Annual Investigators' Workshop The 10th DOE Low Dose Radiation Research Program...

289

Low Dose Investigator one of Canada's Top 40  

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Low Dose Investigator one of Canada's Top 40 Olga Kovalchuk Congratulations to Low Dose Radiation Research Program investigator Olga Kovalchuk on being named one of "Canada's Top...

290

Non-Targeted Effects of Low Dose Ionizing Radiation Act Via TGFβ...  

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effect that mediates microenvironment composition. TGF is activated in mouse mammary gland following whole body exposure to doses of as low as 0.1 Gy and persists in the stroma...

291

Non-linear DNA damage response to low-dose ionizing radiation...  

NLE Websites -- All DOE Office Websites (Extended Search)

the spatiotemporal response in ATM- or DNApkcs-deficient cells or as a function of chromatin territory, to extend the work done by the Karpen Lab using the Drosophila model...

292

Low Dose Suppression of Neoplastic Transformation in Vitro  

SciTech Connect

This grant was to study the low dose suppression of neoplastic transformation in vitro and the shape of the dose-response curve at low doses and dose-rates of ionizing radiation. Previous findings had indicated a suppression of transformation at dose <10cGy of low-LET radiation when delivered at high dose-rate. The present study indicates that such suppression extends out to doses in excess of 100cGy when the dose (from I-125 photons) is delivered at dose-rates as low as 0.2 mGy/min and out to in excess of {approx}25cGy the highest dose studied at the very low dose-rate of 0.5 mGy/day. We also examined dose-rate effects for high energy protons (which are a low-LET radiation) and suppression was evident below {approx}10cGy for high dose-rate delivery and at least out to 50cGy for low dose-rate (20cGy/h) delivery. Finally, we also examined the effect of low doses of 1 GeV/n iron ions (a high-LET radiation) delivered at high dose-rate on transformation at low doses and found a suppression below {approx}10cGy that could be attributable to an adaptive response in bystander cells induced by the associated low-LET delta rays. These results have implications for cancer risk assessment at low doses.

John Leslie Redpath

2012-05-01T23:59:59.000Z

293

Transcriptional and Epigenetic Responses of Human Cells to Low Dose  

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Transcriptional and Epigenetic Responses of Human Cells to Low Dose Transcriptional and Epigenetic Responses of Human Cells to Low Dose Ionizing Radiation Identified through High Throughput ChIP-Seq Analysis Carl Anderson Brookhaven National Laboratory Abstract The major consequence of human exposures to ionizing radiation (IR) is considered to be an increased incidence of cancer (Brenner et al., 2003). Exposure of cells to 1 Gy of IR produces approximately 40 double-stranded breaks, 1000 single-stranded breaks, and 1000 damaged bases per genome equivalent (Pandita and Richardson, 2009); however, most direct DNA damage is rapidly repaired. Exposure to IR also induces epigenetic changes including both increases and decreases in DNA methylation, and increasing evidence suggests that epigenetic changes can both initiate cancer and

294

NIST Ionizing Radiation Division 2000 - Future Directions  

Science Conference Proceedings (OSTI)

... will enable dose-reconstruction studies for populations exposed at the natural background levels of ionizing radiation. Calibrations of Low-Energy ...

295

DNA Damage Pathways: Low Dose Response and Cross-Talk in TGFβ and ATM  

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Damage Pathways: Low Dose Response and Cross-Talk in TGFβ and ATM Damage Pathways: Low Dose Response and Cross-Talk in TGFβ and ATM Signaling Peter O'Neill University of Oxford Abstract The ATM and TGFbeta signal transduction pathways are essential to cellular and tissue control responses to ionizing radiation (IR) and aberrant modifications to these pathways are extensive in cancer. We hypothesize that the ATM and TGFbeta signaling pathways are fully induced at high doses of acute low-LET radiation, whereas only partially induced at low doses. As a consequence of partial stimulation of these pathways important questions arise not only on the validity of the linear no-threshold assumption used in radiation regulations, but also on our ability to extrapolate experimental and human epidemiology data from high to low doses. The

296

Low-dose, low-LET γ-radiation alters carcinogen-induced splenic cytokine production and immune cell phenotype of A/J mice  

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dose, low-LET γ-radiation alters carcinogen-induced splenic cytokine production and immune cell dose, low-LET γ-radiation alters carcinogen-induced splenic cytokine production and immune cell phenotype of A/J mice. V. Gonzales, K. Gott, M. Makvandi, N. Kikendall, A. Monier, E. Maloy, C. Rietz, B. Scott and J. Wilder. Lovelace Respiratory Research Institute, Albuquerque, NM. Introduction: Low-dose, low-linear-energy-transfer (LET) radiation (LDR; < 100 mGy) activates the immune response, presumably via epigenetic pathways (Scott et al. 2009) and may lead to cancer suppression (Nowosielska et al. 2006). One of the mechanisms by which it might do so is by altering the cytokines produced after carcinogen and/or radiation exposure. Alternatively, LDR may activate

297

Identification of novel ionizing radiation signaling targets in reconstituted human skin  

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of novel ionizing radiation signaling targets in reconstituted human skin of novel ionizing radiation signaling targets in reconstituted human skin Feng Yang, Katrina M. Waters, Bobbie-Jo Webb-Robertson, Lye-Meng Markillie, Rachel M. Wirgau, Shawna M. Hengel, Ljiljana Pasa-Tolic, and David L. Stenoien. Pacific Northwest National Laboratory Our focus has been on identifying the early events that occur after low dose ionizing radiation exposure that precede and often regulate downstream events such as altered transcription, protein secretion and epigenetic regulation. Phosphorylation is one of the earliest detectible events that occurs following radiation exposure and plays important roles in multiple biological pathways including DNA damage repair, transcription, apoptosis, and cell cycle progression. Very robust

298

Latest Research, Effects from Low Dose Exposure, Response to...  

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Program Draft of Low Dose Program History 1998-2008 Available for Review and Comment The history of the first decade of DOE Low Dose Radiation Research Program has been written by...

299

Induction of DNA Damage by Low Dose PET scans  

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Induction of DNA Damage by Low Dose PET scans Induction of DNA Damage by Low Dose PET scans Douglas Boreham McMaster University Abstract This research is focused on assessing the radiation risk associated with positron emission tomography (PET) scans. It has been suggested that low dose medical imaging, such as PET scans, carry an added biological risk because they expose the patient to ionizing radiation. PET scanning is an increasingly used nuclear medicine procedure that requires the administration of isotope 18F-fluorodeoxyglucose (18F-FDG, E=250 keV β and 511 keV γ) and results in an effective dose to the patient ranging from 7-22 mSv. The radiation induced DNA damage associated with a PET scan was studied in 7-9 week old female wild type Trp53 +/+ mice. Mice were given a PET scan with 18F-FDG and the biological response was assessed in bone marrow using

300

Radiation and litigation : analyses of the ALARA principle and low dose radiation in the courts, and the future of radiation in court cases  

E-Print Network (OSTI)

Currently there are a growing number of radiation workers. In order to ensure the safety of the employees, regulations have been established by the federal government and state governments to limit the dose equivalent to ...

Esparza, Enrique

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

THE HEALTH EFFECTS IN WOMEN EXPOSED TO LOW-LEVELS OF IONIZING RADIATION  

E-Print Network (OSTI)

and Effects of Ionizing Radiation. New York, United Nations,Effects of Ionizing Radiation (BEIR III). The EffectsLevels of Ionizing Radiation. Washington, D.C. , National

Fabrikant, J.I.

2010-01-01T23:59:59.000Z

302

Real-time Molecular Study of Bystander Effects of Low dose Low LET radiation Using Living Cell Imaging and Nanoparticale Optics  

SciTech Connect

In this study two novel approaches are proposed to investigate precisely the low dose low LET radiation damage and its effect on bystander cells in real time. First, a flow shear model system, which would provide us a near in vivo situation where endothelial cells in the presence of extra cellular matrix experiencing continuous flow shear stress, will be used. Endothelial cells on matri-gel (simulated extra cellular matrix) will be subjected to physiological flow shear (that occurs in normal blood vessels). Second, a unique tool (Single nano particle/single live cell/single molecule microscopy and spectroscopy; Figure A) will be used to track the molecular trafficking by single live cell imaging. Single molecule chemical microscopy allows one to single out and study rare events that otherwise might be lost in assembled average measurement, and monitor many target single molecules simultaneously in real-time. Multi color single novel metal nanoparticle probes allow one to prepare multicolor probes (Figure B) to monitor many single components (events) simultaneously and perform multi-complex analysis in real-time. These nano-particles resist to photo bleaching and hence serve as probes for unlimited timeframe of analysis. Single live cell microscopy allows one to image many single cells simultaneously in real-time. With the combination of these unique tools, we will be able to study under near-physiological conditions the cellular and sub-cellular responses (even subtle changes at one molecule level) to low and very low doses of low LET radiation in real time (milli-second or nano-second) at sub-10 nanometer spatial resolution. This would allow us to precisely identify, at least in part, the molecular mediators that are responsible of radiation damage in the irradiated cells and the mediators that are responsible for initiating the signaling in the neighboring cells. Endothelial cells subjected to flow shear (2 dynes/cm2 or 16 dynes/cm2) and exposed to 0.1, 1 and 10 cGy on coverslips will be examined for (a) low LET radiation-induced alterations of cellular function and its physiological relevance in real time; and (b) radiation damage triggered bystander effect on the neighboring unirradiated cells. First, to determine the low LET radiation induced alteration of cellular function we will examine: (i) the real time transformation of single membrane transporters in single living cells; (ii) the pump efficiency of membrane efflux pump of live cells in real time at the molecular level; (iii) the kinetics of single-ligand receptor interaction on single live cell surface (Figure C); and (iv) alteration in chromosome replication in living cell. Second, to study the radiation triggered bystander responses, we will examine one of the key signaling pathway i.e. TNF- alpha/NF-kappa B mediated signaling. TNF-alpha specific nano particle sensors (green) will be developed to detect the releasing dynamics, transport mechanisms and ligand-receptor binding on live cell surface in real time. A second sensor (blue) will be developed to simultaneously monitor the track of NF-kB inside the cell. The proposed nano-particle optics approach would complement our DOE funded study on biochemical mechanisms of TNF-alpha- NF-kappa B-mediated bystander effect.

Natarajan, Mohan [UT Health Science Center at San Antonio; Xu, Nancy R [Old Dominion University; Mohan, Sumathy [UT Health Science Center at San Antonio

2013-06-03T23:59:59.000Z

303

Ionization of Rydberg atoms by blackbody radiation  

E-Print Network (OSTI)

We have studied an ionization of alkali-metal Rydberg atoms by blackbody radiation (BBR). The results of the theoretical calculations of ionization rates of Li, Na, K, Rb and Cs Rydberg atoms are presented. Calculations have been performed for nS, nP and nD states which are commonly used in a variety of experiments, at principal quantum numbers n=8-65 and at the three ambient temperatures of 77, 300 and 600 K. A peculiarity of our calculations is that we take into account the contributions of BBR-induced redistribution of population between Rydberg states prior to photoionization and field ionization by extraction electric field pulses. The obtained results show that these phenomena affect both the magnitude of measured ionization rates and shapes of their dependences on n. A Cooper minimum for BBR-induced transitions between bound Rydberg states of Li has been found. The calculated ionization rates are compared with our earlier measurements of BBR-induced ionization rates of Na nS and nD Rydberg states with ...

Beterov, I I; Ryabtsev, I I; Entin, V M; Ekers, A; Bezuglov, N N

2008-01-01T23:59:59.000Z

304

In Vivo Effects of Low Dose γ-Rays on Mitochondrial Function  

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In Vivo Effects of Low Dose γ-Rays on Mitochondrial Function In Vivo Effects of Low Dose γ-Rays on Mitochondrial Function Edouard Azzam New Jersey Medical School Cancer Center Abstract Mitochondria consume about 90% of the body’s oxygen and are the richest source of reactive oxygen species (ROS). They play an integral part in signaling events that occur in response to oxidizing agents, including ionizing radiation. To gain insight into radiation-induced effects on mitochondria, we investigated the in vivo effects of low dose γ-rays on mitochondrial protein import, aconitase activity and modulation of antioxidants in tissues of whole body-irradiated mice. Mitochondrial protein import is a fundamental mechanism of mitochondrial biogenesis, and the TCA cycle in the mitochondrial matrix is a central pathway of oxidative

305

Low Dose Responses in Human Cells and Molecular Mechanisms of Adaptation  

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Responses in Human Cells and Molecular Mechanisms of Adaptation Responses in Human Cells and Molecular Mechanisms of Adaptation Joe Gray Priscilla Cooper Lawrence Berkeley National Laboratory Abstract The radiation Adaptive Response (adaptation, or AR) is a well documented, although evidently highly variable, protective phenomenon in which exposures to low-dose or low-dose-rate ionizing radiation result in reduced deleterious effects of subsequent higher exposures. Protection has been reported against a variety of biologically important endpoints, but its variability as a function of cell and tissue type and its genetic control are not well understood. The adaptive response is predicted to result in a non-linear dose response for cancer risk in the low dose range. However, the molecular mechanism(s) remain unknown, and such information is

306

Low Dose Program Highlight: Do Heritable Differences in an Individual...  

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Differences in an Individual's Immune System Predict Differential Sensitivity to Low Dose Radiation Exposure? Brynn Voy, University of Tennessee Knoxville, and Oak Ridge...

307

Composite scintillators for detection of ionizing radiation  

DOE Patents (OSTI)

Applicant's present invention is a composite scintillator having enhanced transparency for detecting ionizing radiation comprising a material having optical transparency wherein said material comprises nano-sized objects having a size in at least one dimension that is less than the wavelength of light emitted by the composite scintillator wherein the composite scintillator is designed to have selected properties suitable for a particular application.

Dai, Sheng (Knoxville, TN); Stephan, Andrew Curtis (Knoxville, TN); Brown, Suree S. (Knoxville, TN); Wallace, Steven A. (Knoxville, TN); Rondinone, Adam J. (Knoxville, TN)

2010-12-28T23:59:59.000Z

308

Alloy nanoparticle synthesis using ionizing radiation  

DOE Patents (OSTI)

A method of forming stable nanoparticles comprising substantially uniform alloys of metals. A high dose of ionizing radiation is used to generate high concentrations of solvated electrons and optionally radical reducing species that rapidly reduce a mixture of metal ion source species to form alloy nanoparticles. The method can make uniform alloy nanoparticles from normally immiscible metals by overcoming the thermodynamic limitations that would preferentially produce core-shell nanoparticles.

Nenoff, Tina M. (Sandia Park, NM); Powers, Dana A. (Albuquerque, NM); Zhang, Zhenyuan (Durham, NC)

2011-08-16T23:59:59.000Z

309

Waveshifters and Scintillators for Ionizing Radiation Detection  

Science Conference Proceedings (OSTI)

Scintillation and waveshifter materials have been developed for the detection of ionizing radiation in an STTR program between Ludlum Measurements, Inc. and the University of Notre Dame. Several new waveshifter materials have been developed which are comparable in efficiency and faster in fluorescence decay than the standard material Y11 (K27) used in particle physics for several decades. Additionally, new scintillation materials useful for fiber tracking have been developed which have been compared to 3HF. Lastly, work was done on developing liquid scintillators and paint-on scintillators and waveshifters for high radiation environments.

B.Baumgaugh; J.Bishop; D.Karmgard; J.Marchant; M.McKenna; R.Ruchti; M.Vigneault; L.Hernandez; C.Hurlbut

2007-12-11T23:59:59.000Z

310

Development and characterization of a novel variable low-dose rate irradiator for in vivo mouse studies  

E-Print Network (OSTI)

Radiation exposure of humans generally results in low doses delivered at low dose rate. Our limited knowledge of the biological effects of low dose radiation is mainly based on data from the atomic bomb Life Span Study ...

Davidson, Matthew Allen

311

Low dose irradiation of the early vertebrate embryo and early onset of  

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irradiation of the early vertebrate embryo and early onset of irradiation of the early vertebrate embryo and early onset of tissue aging Lingling Ding Georgia Health Sciences University Abstract There is considerable overlap between cellular and molecular changes that occur in response to low doses of ionizing radiation and those that occur during aging. Both processes are characterized by accumulation of persistent DNA damage (“wear and tear” on the genome), persistent oxidative stress, and depletion of stem/progenitor cells, leading to loss of repair and regenerative capacity. Here we test a hypothesis that exposure to low-dose ionizing radiation accelerates normal, aging-related tissue changes. The work was performed using a small, genetically tractable vertebrate model organism, the Japanese medaka fish (Oryzias latipes). Medaka have a

312

Dosimetry for non-ionizing radiation  

SciTech Connect

Several commercially available phosphor-teflon dosimeters were subjected to thermal fade studies. The thermoluminescence dosimetry (TLD) tags were washed and air dried prior to each ionizing radiation pretreatment. Readings made over 3 weeks on the CaF2:Mn and CaF2:Dy TLD tags did not produce measurable thermal-fade characteristics for the 22 to 31/sup 0/C temperature range. Nonionizing radiation treatments were at 2.45 GHz. While the results obtained did demonstrate decreases in signal levels over time and temperature changes, the patterns were not smooth, making it impossible to establish differences in TLD readings as quantitative measures of temperature differences which could serve as measures of long-term exposures to nonionizing radiation. The authors concluded that, due to the irregularities that existed in the thermal fade characteristics, the dosimeter would not be suitable for quantifying exposures to nonionizing radiation. There was significant potential in the device, however, as an indicator of radiation leakage or exposure.

Fanslow, G.E.

1981-03-01T23:59:59.000Z

313

NIST Ionizing Radiat. Div. - 2004: Strategic Focus 1  

Science Conference Proceedings (OSTI)

... and to determine exposure or dose-rate values ... to maximize the energy absorption of the ionizing radiation. ... the calibration of an 55 Fe low-energy x ...

314

Radiation Risk from Chronic Low Dose-Rate Radiation Exposures: The Role of Life-Time Animal Studies - Workshop October 2005  

SciTech Connect

As a part of Radiation research conference, a workshop was held on life-long exposure studies conducted in the course of irradiation experiements done at Argonne National Laboratory between 1952-1992. A recent review article documents many of the issues discussed at that workshop.

Gayle Woloschak

2009-12-16T23:59:59.000Z

315

The Effects of Low Dose Irradiation on Inflammatory Response Proteins in a 3D Reconstituted Human Skin Tissue Model  

Science Conference Proceedings (OSTI)

Skin responses to moderate and high doses of ionizing radiation include the induction of DNA repair, apoptosis, and stress response pathways. Additionally, numerous studies indicate that radiation exposure leads to inflammatory responses in skin cells and tissue. However, the inflammatory response of skin tissue to low dose radiation (<10 cGy) is poorly understood. In order to address this, we have utilized a reconstituted human skin tissue model (MatTek EpiDerm FT) and assessed changes in 23 cytokines twenty-four and forty eight hours following treatment of skin with either 3 or 10 cGy low-dose of radiation. Three cytokines, IFN-?, IL-2, MIP-1?, were significantly altered in response to low dose radiation. In contrast, seven cytokines were significantly altered in response to a high radiation dose of 200 cGy (IL-2, IL-10, IL-13, IFN-?, MIP-1?, TNF ?, and VEGF) or the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (G-CSF, GM-CSF, IL-1?, IL-8, MIP-1?, MIP-1?, RANTES). Additionally, radiation induced inflammation appears to have a distinct cytokine response relative to the non-radiation induced stressor, TPA. Overall, these results indicate that there are subtle changes in the inflammatory protein levels following exposure to low dose radiation and this response is a sub-set of what is seen following a high dose in a human skin tissue model.

Varnum, Susan M.; Springer, David L.; Chaffee, Mary E.; Lien, Katie A.; Webb-Robertson, Bobbie-Jo M.; Waters, Katrina M.; Sacksteder, Colette A.

2012-12-01T23:59:59.000Z

316

22.01 Introduction to Ionizing Radiation, Fall 2003  

E-Print Network (OSTI)

Introduction to basic properties of ionizing radiations and their uses in medicine, industry, science, and environmental studies. Discusses natural and man-made radiation sources, energy deposition and dose calculations, ...

Coderre, Jeffrey A.

317

Effects of prenatal exposure to ionizing radiation  

SciTech Connect

Prenatal exposure to ionizing radiation induces some effects that are seen at birth and others that cannot be detected until later in life. Data from A-bomb survivors in Hiroshima and Nagasaki show a diminished number of births after exposure under 4 wk of gestational age. Although a wide array of congenital malformations has been found in animal experimentation after such exposure to x rays, in humans only small head size (exposure at 4-17 wk) and mental retardation (exposure primarily at 8-15 wk) have been observed. In Hiroshima, small head size occurred after doses of 0.10-0.19 Gy or more, and an excess of mental retardation at 0.2-0.4 Gy or more. Intelligence test scores were reduced among A-bomb survivors exposed at 8-15 wk of gestational age by 21-29 IQ points per Gy. Other effects of in-utero exposure to atomic radiation include long-lasting complex chromosome abnormalities.

Miller, R.W. (National Cancer Institute, Bethesda, MD (USA))

1990-07-01T23:59:59.000Z

318

Microsoft PowerPoint - Low Dose Update Metting 6 Dec 2012  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Low Dose DOE's Low Dose Low Dose DOE's Low Dose R di ti R h R di ti R h Radiation Research Radiation Research Program Program g g NF Metting, Sc.D., Program Manager Nuclear Energy Advisory Committee Meeting Nuclear Energy Advisory Committee Meeting L'Enfant Plaza Hotel L'Enfant Plaza Hotel 6 December 2012 Office of Science Office of Biological and Environmental Research DOE's Low Dose Program: DOE s Low Dose Program: Is unique within the U.S. government in focusing on low dose biological research aimed at informing current and future g g national radiation risk policy for the public and the workplace * DOE focuses on worker and public safety from very low dose x- and p y y gamma-ray exposures encountered in energy production and environmental cleanup In contrast: In contrast: * NASA focuses on astronaut safety from high energy particulate radiation

319

PROTRACTED LOW DOSE PHOTON AND SIMULATED SOLAR FLARE  

NLE Websites -- All DOE Office Websites (Extended Search)

PROTRACTED LOW DOSE PHOTON AND SIMULATED SOLAR FLARE PROTRACTED LOW DOSE PHOTON AND SIMULATED SOLAR FLARE PROTON EFFECTS ON CYTOKINE/CHEMOKINE EXPRESSION AFTER WHOLE-BODY IRRADIATION Asma Rizvi 2 , George Coutrakon 1 , James M. Slater 1 , Michael J. Pecaut 1,2 and Daila S. Gridley 1,2 Departments. of 1 Radiation Medicine and 2 Biochemistry & Microbiology Loma Linda University & Medical Center, Loma Linda, CA 92354 Astronauts are exposed to low dose/low dose rate radiation (LDR) and may also be acutely irradiated during a solar particle event (SPE). The biological effects of LDR alone and when combined with a solar particle event, are not yet clearly understood. Previous studies have shown that irradiation can have adverse effects on T cells. The reactive oxygen species (ROS) that are produced as a result of radiation can alter or damage the

320

Low Dose Studies with Focused X-Rays in cell and Tissue Models: Mechanisms of Bystander and Genomic Instability Responses  

SciTech Connect

The management of the risks of exposure of people to ionizing radiation is important in relation to its uses in industry and medicine, also to natural and man-made radiation in the environment. The vase majority of exposures are at a very low level of radiation dose. The risks are of inducing cancer in the exposed individuals and a smaller risk of inducing genetic damage that can be indicate that they are low. As a result, the risks are impossible to detect in population studies with any accuracy above the normal levels of cancer and genetic defects unless the dose levels are high. In practice, this means that our knowledge depends very largely on the information gained from the follow-up of the survivors of the atomic bombs dropped on Japanese cities. The risks calculated from these high-dose short-duration exposures then have to be projected down to the low-dose long-term exposures that apply generally. Recent research using cells in culture has revealed that the relationship between high- and low-dose biological damage may be much more complex than had previously been thought. The aims of this and other projects in the DOE's Low-Dose Program are to gain an understanding of the biological actions of low-dose radiation, ultimately to provide information that will lead to more accurate quantification of low-dose risk. Our project is based on the concept that the processes by which radiation induces cancer start where the individual tracks of radiation impact on cells and tissues. At the dose levels of most low-dose exposures, these events are rare and any individual cells only ''sees'' radiation tracks at intervals averaging from weeks to years apart. This contrasts with the atomic bomb exposures where, on average, each cell was hit by hundreds of tracks instantaneously. We have therefore developed microbeam techniques that enable us to target cells in culture with any numbers of tracks, from one upwards. This approach enables us to study the biological ha sis of the relationship between high- and low-dose exposures. The targeting approach also allows us to study very clearly a newly recognized effect of radiation, the ''bystander effect'', which appears to dominate some low-dose responses and therefore may have a significant role in low-dose risk mechanisms. Our project also addresses the concept that the background of naturally occurring oxidative damage that takes place continually in cells due to byproducts of metabolism may play a role in low-dose radiation risk. This project therefore also examines how cells are damaged by treatments that modify the levels of oxidative damage, either alone or in combination with low-dose irradiation. In this project, we have used human and rodent cell lines and each set of experiments has been carried out on a single cell type. However, low-dose research has to extend into tissues because signaling between cells of different types is likely to influence the responses. Our studies have therefore also included microbeam experiments using a model tissue system that consists of an explant of a small piece of pig ureter grown in culture. The structure of this tissue is similar to that of epithelium and therefore it relates to the tissues in which carcinoma arises. Our studies have been able to measure bystander-induced changes in the cells growing out from the tissue fragment after it has been targeted with a few radiation tracks to mimic a low-dose exposure.

Kathy Held; Kevin Prise; Barry Michael; Melvyn Folkard

2002-12-14T23:59:59.000Z

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Rules and Regulations for Control of Ionizing Radiation (Arkansas) |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Rules and Regulations for Control of Ionizing Radiation (Arkansas) Rules and Regulations for Control of Ionizing Radiation (Arkansas) Rules and Regulations for Control of Ionizing Radiation (Arkansas) < Back Eligibility Utility Program Info State Arkansas Program Type Environmental Regulations Siting and Permitting Provider Department of Health The Rules and Regulations for Control of Ionizing Radiation are the Arkansas state laws made in accordance the federal Nuclear Regulatory Commission Rules. Any contractor with the US DOE or US Nuclear Regulatory Commission is exempt from the state laws. This set of rules and regulations basically restates the federal policy to ensure that Arkansas is in compliance with the federal standards governing nuclear energy. Specifically the State rules are equivalent to Nuclear Regulatory

322

Carcinogenesis and low-level ionizing radiation with special reference to lung cancer and exposure to radon daughters  

SciTech Connect

Of the important health effects of ionizing radiation, three important late effects - carcinogenesis, teratogenesis and mutagenesis are of greatest concern. This is because any exposure, even at low levels, carries some risk of such deleterious effects. As the dose of radiation increases above very low levels, the risk of health effects increases. Cancer-induction is the most important late somatic effect of low-dose ionizing radiation. Solid cancers, rather than leukemia, are principal late effects in exposed individuals. Tissues vary greatly in their susceptibility to radiation carcinogenesis. The most frequently occurring radiation-induced cancers in man include, in decreasing order of susceptibility: the female breast, the thyroid gland, the blood-forming tissues, the lung, certain organs of the gastrointestinal tract, and the bones. A number of biological and physical factors affect the cancer risk, such as age, sex, life-style, LET, and RBE. Despite uncertainty about low-level radiation risks, regulatory and advisory bodies must set standards for exposure, and individuals need information to be able to make informed judgments for themselves. From the point of view of the policy maker, the overriding concern is the fact that small doses of radiation can cause people to have more cancers than would otherwise be expected. While concern for all radiation effects exists, our human experience is limited to cancer-induction in exposed populations. This discussion is limited to cancer risk estimation and decision-making in relation to the health effects on populations of exposure to low levels of ionizing radiation. Here, low-level radiation will refer to yearly whole-body doses up to 5 rems or 0.05 Sv, or to cumulative doses up to 50 rems or 0.5 Sv from low-LET radiation and from high-LET radiation. (ERB)

Fabrikant, J.I.

1982-04-01T23:59:59.000Z

323

Low Dose Studies with Focused X-rays in Cell and Tissue Models: Mechanisms of Bystander and Genomic Instability Responses  

SciTech Connect

The management of the risks of exposure of people to ionizing radiation is important in relation to its uses in industry and medicine, also to natural and man-made radiation in the environment. The vase majority of exposures are at a very low level of radiation dose. The risks are of inducing cancer in the exposed individuals and a smaller risk of inducing genetic damage that can be transmitted to children conceived after exposure. Studies of these risks in exposed population studies with any accuracy above the normal levels of cancer and genetic defects unless the dose levels are high. In practice, this means that our knowledge depends very largely on the information gained from the follow-up of the survivors of the atomic bombs dropped on Japanese cities. The risks calculated from these high-dose short-duration exposures then have to be projected down to the low-dose long-term exposures that apply generally. Recent research using cells in culture has revealed that the relations hi between high- and low-dose biological damage may be much more complex than had previously been thought. The aims of this and other projects in the DOE's Low-Dose Program are to gain an understanding of the biological actions of low-dose radiation, ultimately to provide information that will lead to more accurate quantification of low-dose risk. Our project is based on the concept that the processes by which radiation induces cancer start where the individual tracks of radiation impact on cells and tissues. At the dose levels of most low-dose exposures, these events are rare and any individual cells only ''sees'' radiation tracks at intervals averaging from weeks to years apart. This contracts with the atomic bomb exposures where, on average, each cell was hit by hundreds of tracks instantaneously. We have therefore developed microbeam techniques that enable us to target cells in culture with any number of tracks, from one upwards. This approach enables us to study the biological basis of the relationship between high- and low-dose exposures. The targeting approach also allows us to study very clearly a newly recognized effect of radiation, the ''bystander effect'', which appears to dominate some low-dose responses and therefore may have a significant role in low-dose risk mechanisms. Our project also addresses the concept that the background of naturally occurring oxidative damage that takes place continually in cells due to byproducts of metabolism may play a role in treatments that modify the levels of oxidative damage, either alone or in combination with low-dose irradiation. In this project, we have used human and rodent cell lines and each set of experiments has been carried out on a single cell type. However, low-dose research has to extend into tissues because signaling between cells of different types is likely to influence the responses. Our studies have therefore also included microbeam experiments using a model tissue system that consists of an explant of a small piece of pig ureter grown in culture. The structure of this tissue is similar to that of epithelium and there it relates to the tissues in which carcinoma arises. Our studies have been able to measure bystander-induced changes in the cells growing out from the tissue fragment after it has been targeted with a few radiation tracks to mimic a low-dose exposure.

Barry D. Michael; Kathryn Held; Kevin Prise

2002-12-19T23:59:59.000Z

324

Radiation leukaemogenesis at low doses DE-FG02-05 ER 63947 Final Technical Report 15 May 2005 ???????????????¢???????????????????????????????? 14 May 2010  

Science Conference Proceedings (OSTI)

This report provides a complete summary of the work undertaken and results obtained under US Department of Energy grant DF-FG02-05 ER 63947, Radiation leukaemogenesis at low doses. There is ample epidemiological evidence indicating that ionizing radiation is carcinogenic in the higher dose range. This evidence, however, weakens and carries increasing uncertainties at doses below 100-200 mSv. At these low dose levels the form of the dose-response curve for radiation-induced cancer cannot be determined reliably or directly from studies of human populations. Therefore animal, cellular and other experimental systems must be employed to provide supporting evidence on which to base judgements of risk at low doses. Currently in radiological protection a linear non-threshold (LNT) extrapolation of risk estimates derived from human epidemiological studies is used to estimate risks in the dose range of interest for protection purposes. Myeloid leukaemias feature prominently among the cancers associated with human exposures to ionising radiation (eg UNSCEAR 2006; IARC 2000). Good animal models of radiation-induced acute myeloid leukaemia (AML) are available including strains such as CBA, RFM and SJL (eg Major and Mole 1978; Ullrich et al 1976; Resnitzky et al 1985). Early mechanistic studies using cytogenetic methods in these mouse models established that the majority of radiation-induced AMLs carried substantial interstitial deletions in one copy of chromosome (chr) 2 (eg Hayata et al 1983; Trakhtenbrot et al 1988; Breckon et al 1991; Rithidech et al 1993; Bouffler et al 1996). Chr2 aberrations are known to occur in bone marrow cells as early as 24 hours after in vivo irradiation (Bouffler et al 1997). Subsequent molecular mapping studies defined a distinct region of chr2 that is commonly lost in AMLs (Clark et al 1996; Silver et al 1999). Further, more detailed, analysis identified point mutations at a specific region of the Sfpi1/PU.1 haemopoietic transcription factor gene which lies in the commonly deleted region of chr2 (Cook et al 2004; Suraweera et al 2005). These lines of evidence strongly implicate the Sfpi1/PU.1 gene as a tumour suppressor gene, dysregulation of which leads to myeloid leukaemia. The main focus of this project was to utilize the CBA mouse model of radiation leukaemogenesis to explore mechanisms of low dose and low dose-rate leukaemogenesis. A series of mechanistic investigations were undertaken, the central aim of which was to identify the events that convert normal cells into myeloid leukaemia cells and explore the dose-response relationships for these. Much of the work centred on the Sfpi1/PU.1 gene and its role in leukaemogenesis. Specific studies considered the dose-response and time-course relationships for loss of the gene, the functional consequences of Sfpi1/PU.1 loss and mutation on transcriptional programmes and developing an in vivo reporter gene system for radiation-induced alterations to PU.1 expression. Additional work sought further genetic changes associated with radiation-induced AMLs and a better characterization of the cell of origin or 'target cell' for radiation-induced AML. All the information gathered is of potential use in developing biologically realistic mathematical models for low dose cancer risk projection.

Simon Bouffler

2010-07-28T23:59:59.000Z

325

Composite scintillators for detection of ionizing radiation ...  

Building Energy Efficiency; Electricity Transmission; Energy Analysis; Energy ... Fluorescent Nanoparticles for Radiation DetectionFluorescent Nanoparticles for ...

326

NIST Ionizing Radiation Division 1999 - Current Directions  

Science Conference Proceedings (OSTI)

... effect relationships for radiation-induced stochastic ... validate the EPR dose assessment methods ... Calibration of Low-Energy Photon Brachytherapy ...

327

The ionizing radiation environment in space and its effects  

Science Conference Proceedings (OSTI)

The ionizing radiation environment in space poses a hazard for spacecraft and space crews. The hazardous components of this environment are reviewed and those which contribute to radiation hazards and effects identified. Avoiding the adverse effects of space radiation requires design, planning, monitoring and management. Radiation effects on spacecraft are avoided largely though spacecraft design. Managing radiation exposures of space crews involves not only protective spacecraft design and careful mission planning. Exposures must be managed in real time. The now-casting and forecasting needed to effectively manage crew exposures is presented. The techniques used and the space environment modeling needed to implement these techniques are discussed.

Adams, Jim; Falconer, David; Fry, Dan [Center for Space Plasma and Aeronomic Research (CSPAR), UA Huntsville (United States); Space Radiation Analysis Group, NASA Johnson Space Center (United States)

2012-11-20T23:59:59.000Z

328

NIST Ionizing Radiation Division 2001 - Program Directions  

Science Conference Proceedings (OSTI)

... seen a tremendous increase in the use of low-energy photon ... for the high levels of absorbed dose used in the industrial radiation processing of ...

329

NIST Ionizing Radiation Division 1998 - Current Directions  

Science Conference Proceedings (OSTI)

... Cs gamma-ray ranges, and the low-energy photon ... beam, and a high-dose- rate Gammacell used in our radiation-processing dosimetry ...

330

Concerns with low-level ionizing radiation  

SciTech Connect

Populations have been studied in geographic areas of increased natural radiation, in radiation-exposed workers, in patients medically exposed, and in accidental exposures. No reproducible evidence exists of harmful effects from increases in background radiation three to ten times the usual levels. There is no increase in leukemia or other cancers among American military participants in nuclear testing, no increase in leukemia or thyroid cancer among medical patients receiving {sup 131}I for diagnosis or treatment of hypothyroidism, and no increase in lung cancer among nonsmokers exposed to increased radon in the home. The association of radiation with the atomic bomb and with excessive regulatory and health physics as-low-as-reasonably-achievable (ALARA) radiation levels practices has created a climate of fear about the dangers of radiation at any level. However, there is no evidence that radiation exposures at the levels equivalent to medical usage are harmful. The unjustified excessive concern with radiation at any level, however, precludes beneficial uses of radiation and radioactivity in medicine, science, and industry.

Yalow, R.S.

1994-12-31T23:59:59.000Z

331

Low dose radiation and cancer in A-bomb survivors: latency and non-linear dose-response in the 195090 mortality cohort  

E-Print Network (OSTI)

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background: Analyses of Japanese A-bomb survivors ' cancer mortality risks are used to establish recommended annual dose limits, currently set at 1 mSv (public) and 20 mSv (occupational). Do radiation doses below 20 mSv have significant impact on cancer mortality in Japanese A-bomb survivors, and is the dose-response linear? Methods: I analyse stomach, liver, lung, colon, uterus, and all-solid cancer mortality in the 0 20 mSv colon dose subcohort of the 195090 (grouped) mortality cohort, by Poisson regression using a time-lagged colon dose to detect latency, while controlling for gender, attained age, and age-atexposure. I compare linear and non-linear models, including one adapted from the cellular bystander effect for ? particles. Results: With a lagged linear model, Excess Relative Risk (ERR) for the liver and all-solid cancers is significantly positive and several orders of magnitude above extrapolations from the Life Span Study Report 12 analysis of the full cohort. Non-linear models are strongly superior to the linear model for the stomach (latency 11.89 years), liver (36.90), lung (13.60) and all-solid (43.86) in fitting

Greg Dropkin; Greg Dropkin

2007-01-01T23:59:59.000Z

332

Ionic Liquids and Ionizing Radiation: Reactivity of Highly Energetic  

NLE Websites -- All DOE Office Websites (Extended Search)

Ionizing Radiation: Reactivity of Highly Energetic Ionizing Radiation: Reactivity of Highly Energetic Species James F. Wishart J. Phys. Chem. Lett. 1, 3225-3231 (2010). [Find paper at ACS Publications] or use ACS Articles on Request View the video on this Perspective article at The Journal of Physical Chemistry Letters (5:03) Selected for the ACS Special Virtual Issue on Ionic Liquids (March 2011). Abstract: Due to their unique properties, ionic liquids present many opportunities for basic research on the interactions of radiation with materials under conditions not previously available. At the same time, there are practical applied reasons for characterizing, understanding, and being able to predict how ionic-liquid-based devices and industrial-scale systems will perform under conditions of extreme reactivity, including radiation. This

333

Consequences of low dose irradiation in the CNS  

NLE Websites -- All DOE Office Websites (Extended Search)

Consequences of low dose irradiation in the CNS Consequences of low dose irradiation in the CNS Bertrand Tseng University of California Abstract Radiation-induced oxidative stress can impact the physiologic function of multipotent neural stem and precursor cells by activating redox-sensitive signaling cascades that can alter radiosensitivity, mitochondrial function, and cell fate. Many of these signaling pathways depend on the nature, magnitude and duration of the specific reactive species involved, features that dictate in large part whether radiation-induced changes are harmful or beneficial to the organism. We have shown that acute low dose irradiation (2-20 cGy) can elicit significant increases in reactive oxygen (ROS) and nitrogen (RNS) species over several days to weeks. These redox changes can

334

Low Dose Radiation Program: Links - Public Involvement  

NLE Websites -- All DOE Office Websites (Extended Search)

Risk Communication, More Than Facts and Feelings IAEA Bulletin The United Nations and Chernobyl U.S. Federal Emergency Management Agency, FACT Sheet - Nuclear Blast Return to...

335

Low Dose Radiation Program: Slide Show  

NLE Websites -- All DOE Office Websites (Extended Search)

1 next > Program Participants and Presenters Investigators' Workshop IX April 12-14, 2010 Washington, DC Program Participants and Presenters Investigators' Workshop IX April 12-14,...

336

Low Dose Radiation Program: Frequently Asked Questions  

NLE Websites -- All DOE Office Websites (Extended Search)

of genetic repair and misrepair mechanisms within cells; this is expected to benefit cancer research, treatment, and prevention. Second, research should decrease uncertainty...

337

Low Dose Radiation Research Program: Douglas Boreham  

NLE Websites -- All DOE Office Websites (Extended Search)

Douglas Boreham McMaster University Past Funded Projects The Adaptive Response i p53 Cancer-prone Mice: Loss of Heterozygosity and Chromosome Instability Technical Abstracts 2003...

338

Low Dose Radiation Research Program: John Minna  

NLE Websites -- All DOE Office Websites (Extended Search)

Minna University of Texas, Southwestern Medical Center John Minna, M.D. Currently Funded Projects Human Lung Cancer Progression Model for HZE Particle Exposure...

339

Low Dose Radiation Research Program: Allan Balmain  

NLE Websites -- All DOE Office Websites (Extended Search)

of genomic instability and epithelial tumor development by the p53-Fbxw7Cdc4 pathway. Cancer Research 65:6488-6492. Perez-Losada, J., Wu, D., DelRosario, R., Balmain, A., and...

340

Low Dose Radiation Research Program: David Schild  

NLE Websites -- All DOE Office Websites (Extended Search)

cells. Nucleic Acids Research 30:1001-1008. Takata, M., Sasaki, M.S., Tachiiri, S., Fukushima, T., Sonoda, E., Schild, D., Thompson, L.H., and Takeda, S. (2001). Chromosome...

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Low Dose Radiation Research Program: Larry Thompson  

NLE Websites -- All DOE Office Websites (Extended Search)

cells. The EMBO Journal 20:5513-5520. Takata, M., Sasaki, M.S., Tachiiri, S., Fukushima, T., Sonoda, E., Schild, D., Thompson, L.H., and Takeda, S. (2001). Chromosome...

342

Computational phenotype prediction of ionizing-radiation-resistant bacteria with a multiple-instance learning model  

Science Conference Proceedings (OSTI)

Ionizing-radiation-resistant bacteria (IRRB) are important in biotechnology. The use of these bacteria for the treatment of radioactive wastes is determined by their surprising capacity of adaptation to radionuclides and a variety of toxic molecules. ... Keywords: ionizing-radiation-resistant bacteria, ionizing-radiation-sensitive bacteria, multiple-instance learning, phenotypic prediction, protein sequences

Sabeur Aridhi; Mondher Maddouri; Haitham Sghaier; Engelbert Mephu Nguifo

2013-08-01T23:59:59.000Z

343

Radioadaptation in Neural Stem Cells Exposed to Low Dose Irradiation  

NLE Websites -- All DOE Office Websites (Extended Search)

Radioadaptation in Neural Stem Cells Exposed to Low Dose Irradiation Radioadaptation in Neural Stem Cells Exposed to Low Dose Irradiation Charles Limoli University of California, Irvine Abstract In the CNS, irradiation of multipotent neural stem and precursor cells has been shown to cause a persistent oxidative stress that impacts radiosensitivity, mitochondrial function, and cell fate. The nature, magnitude and duration of reactive species dictates whether these radiation-induced changes are harmful or beneficial to a variety of in vitro and in vivo endpoints of viability and function. We have shown that acute low dose irradiation (2-10 cGy) can elicit significant increases in reactive oxygen (ROS) and nitrogen (RNS) species over several days post-exposure. These changes can be attenuated when the dose is protracted over several weeks using a 57Co flood source having a surface dose rate of

344

Molecular dissection of the roles of the SOD genes in mammalian response to low dose irradiation  

SciTech Connect

It has been long recognized that a significant fraction of the radiation-induced genetic damage to cells are caused by secondary oxidative species. Internal cellular defense systems against oxidative stress play significant roles in countering genetic damage induced by ionizing radiation. The role of the detoxifying enzymes may be even more prominent in the case of low-dose, low-LET irradiation, as the majority of genetic damage may be caused by secondary oxidative species. In this study we have attempted to decipher the roles of the superoxide dismutase (SOD) genes, which are responsible for detoxifying the superoxide anions. We used adenovirus vectors to deliver RNA interference (RNAi or siRNA) technology to down-regulate the expression levels of the SOD genes. We have also over-expressed the SOD genes by use of recombinant adenovirus vectors. Cells infected with the vectors were then subjected to low dose ?-irradiation. Total RNA were extracted from the exposed cells and the expression of 9000 genes were profiled by use of cDNA microarrays. The result showed that low dose radiation had clear effects on gene expression in HCT116 cells. Both over-expression and down-regulation of the SOD1 gene can change the expression profiles of sub-groups of genes. Close to 200 of the 9000 genes examined showed over two-fold difference in expression under various conditions. Genes with changed expression pattern belong to many categories that include: early growth response, DNA-repair, ion transport, apoptosis, and cytokine response.

Eric Y. Chuang

2006-08-31T23:59:59.000Z

345

Low-Dose Spiral CT Scans for Early Lung Cancer Detection | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Low-Dose Spiral CT Scans for Early Lung Cancer Detection Low-Dose Spiral CT Scans for Early Lung Cancer Detection Low-Dose Spiral CT Scans for Early Lung Cancer Detection Low-dose spiral computed tomography (CT) scanning is a noninvasive medical imaging test that has been used for the early detection of lung cancer for over 16 years (Sone et al. 1998; Henschke et.al. 1999). A low-dose spiral chest CT differs from a full-dose conventional chest CT scan primarily in the amount of radiation emitted during CT scans. Chest CT, in general, requires less radiation exposure than other CT procedures because the air-filled tissues of the lungs are not as dense as the tissues of other organs (i.e., less x-ray radiation is needed to penetrate the lung). Radiation dose can be further reduced with lung cancer screening due to the

346

Development of Pattern Recognition Software for Tracks of Ionizing Radiation In Medipix2-Based  

E-Print Network (OSTI)

, and area monitors to characterize the general background radiation environment harmful to humansDevelopment of Pattern Recognition Software for Tracks of Ionizing Radiation In Medipix2-Based tool for the automated identification and classification of tracks of ionizing radiation as measured

Vilalta, Ricardo

347

Role of ATM kinase in ionizing radiation induced DNA damage response...  

NLE Websites -- All DOE Office Websites (Extended Search)

ATM kinase in ionizing radiation induced DNA damage response in human neural stemprogenitor cells and differentiated cell types Adayabalam Balajee Columbia University Medical...

348

Development of a Low Dose Rate Irradiation Facility for Long...  

NLE Websites -- All DOE Office Websites (Extended Search)

Low Dose Rate Irradiation Facility for Long Term Animal Exposures at Colorado State University Michael Weil Colorado State University Abstract A low dose rate irradiation facility...

349

Ionizing radiation risks to satellite power systems (SPS) workers  

DOE Green Energy (OSTI)

The radiation risks to the health of workers who will construct and maintain solar power satellites in the space environment were examined. For ionizing radiation, the major concern will be late or delayed health effects, particularly the increased risk of radiation-induced cancer. The estimated lifetime risk for cancer is 0.8 to 5.0 excess deaths per 10,000 workers per rad of exposure. Thus, for example, in 10,000 workers who completed ten missions with an exposure of 40 rem per mission, 320 to 2000 additional deaths in excess of the 1640 deaths from normally occurring cancer, would be expected. These estimates would indicate a 20 to 120% increase in cancer deaths in the worker-population. The wide range in these estimates stems from the choice of the risk-projection model and the dose-response relationsip. The choice between a linear and a linear-quadratic dose-response model may alter the risk estimate by a factor of about two. The method of analysis (e.g., relative vs absolute risk model) can alter the risk estimate by an additional factor of three. Choosing different age and sex distributions can further change the estimate by another factor of up to three. The potential genetic consequences could be of significance, but at the present time, sufficient information on the age and sex distribution of the worker population is lacking for precise estimation of risk. The potential teratogenic consequences resulting from radiation are considered significant. Radiation exposure of a pregnant worker could result in developmental abnormalities.

Lyman, J.T.; Ainsworth, E.J.; Alpen, E.L.; Bond, V.; Curtis, S.B.; Fry, R.J.M.; Jackson, K.L.; Nachtwey, S.; Sondhaus, C.; Tobias, C.A.; Fabrikant, J.I.

1980-11-01T23:59:59.000Z

350

The low dose damage response pathways in the mouse mammary glands depends  

NLE Websites -- All DOE Office Websites (Extended Search)

low dose damage response pathways in the mouse mammary glands depends low dose damage response pathways in the mouse mammary glands depends on genotype, tissue compartment, exposure regimen, and sampling times Joe Gray & Andrew Wyrobek Lawrence Berkeley National Laboratory Abstract The objectives of this research are to characterize the early and persistent low-dose and adaptive response (AR) damage surveillance networks in mammary glands of radiation sensitive and resistant strains of mice to identify the molecular signatures/mechanisms associated with nonlinear modifications of risk for mammary gland cancer. Our approach uses low-dose exposure regimens that have been reported to induce mammary gland cancer in sensitive strains to determine whether low-dose induced pathways are differentially expressed in epithelial or stromal cells and to determine

351

Recombining WMAP: constraints on ionizing and resonance radiation at recombination  

E-Print Network (OSTI)

We place new constraints on sources of ionizing and resonance radiation at the epoch of the recombination process using the recent CMB temperature and polarization spectra coming from WMAP. We find that non-standard recombination scenarios are still consistent with the current data. In light of this we study the impact that such models can have on the determination of several cosmological parameters. In particular, the constraints on curvature and baryon density appear to be weakly affected by a modified recombination scheme. However, it may affect the current WMAP constraints on inflationary parameters like the spectral index and its running. Physically motivated models, like those based on primordial black hole or super heavy dark matter decay, are able to provide a good fit to the current data. Future observations in both temperature and polarization will be needed to more stringently test these models.

Rachel Bean; Alessandro Melchiorri; Joe Silk

2003-06-18T23:59:59.000Z

352

CORROSION PRODUCT TRANSPORT AND DEPOSITION UNDER IONIZING RADIATION  

DOE Green Energy (OSTI)

A study was made of corrosion product transport and deposition on Zircaloy-2 and AISI 304 stainless steel in the presence and absence of ionizing radiation. Three 100-hour irradiation tests, using 2-Mev electrons from a Van de Graaff accelerator, and four 100-hour nonradiation tests were performed in 6O0 deg F pressurized water. Data from the seven runs and an additional exploratory run are presented. In addition, complete experimental procedures and descriptions of the apparatus are included. Control of pH was obtained by using H and OH form ion exchange resias for pH 7 and Li and OH form ion exchange resins for pH 10. The major conclusion to be drawn from the present work is that the deposition on Zircaloy-2 at pH 10 and 600 deg F is higher than on AISI 304 stainless steel at the same conditions. (auth)

Thomas, C.C. Jr.; Lacock, H.W.; Cadoff, H.Y. ed.

1958-12-01T23:59:59.000Z

353

The Contribution of Tissue Level Organization to Genomic Stability Following Low Dose/Low Dose Rate Gamma and Proton Irradiation  

Science Conference Proceedings (OSTI)

The formation of functional tissue units is necessary in maintaining homeostasis within living systems, with individual cells contributing to these functional units through their three-dimensional organization with integrin and adhesion proteins to form a complex extra-cellular matrix (ECM). This is of particular importance in those tissues susceptible to radiation-induced tumor formation, such as epithelial glands. The assembly of epithelial cells of the thyroid is critical to their normal receipt of, and response to, incoming signals. Traditional tissue culture and live animals present significant challenges to radiation exposure and continuous sampling, however, the production of bioreactor-engineered tissues aims to bridge this gap by improve capabilities in continuous sampling from the same functional tissue, thereby increasing the ability to extrapolate changes induced by radiation to animals and humans in vivo. Our study proposes that the level of tissue organization will affect the induction and persistence of low dose radiation-induced genomic instability. Rat thyroid cells, grown in vitro as 3D tissue analogs in bioreactors and as 2D flask grown cultures were exposed to acute low dose (1, 5, 10 and 200 cGy) gamma rays. To assess immediate (6 hours) and delayed (up to 30 days) responses post-irradiation, various biological endpoints were studied including cytogenetic analyses, apoptosis analysis and cell viability/cytotoxicity analyses. Data assessing caspase 3/7 activity levels show that, this activity varies with time post radiation and that, overall, 3D cultures display more genomic instability (as shown by the lower levels of apoptosis over time) when compared to the 2D cultures. Variation in cell viability levels were only observed at the intermediate and late time points post radiation. Extensive analysis of chromosomal aberrations will give further insight on the whether the level of tissue organization influences genomic instability patterns after low dose radiation exposure. Cells viability/cytotoxicity analysis data are currently being analyzed to determine how these endpoints are affected under our experimental conditions. The results from this study will be translatable to risk assessment for assigning limits to radiation workers, pre-dosing for more effective radiotherapy and the consequences of long duration space flight. The data from this study has been presented a various scientific meetings/workshops and a manuscript, containing the findings, is currently being prepared for publication. Due to unforeseen challenges in collecting the data and standardizing experimental procedures, the second and third aims have not been completed. However, attempts will be made, based on the availability of funds, to continue this project so that these aims can be satisfied.

Cheryl G. Burrell, Ph.D.

2012-05-14T23:59:59.000Z

354

IONIZING RADIATION RISKS TO SATELLITE POWER SYSTEMS (SPS) WORKERS  

E-Print Network (OSTI)

and A. I. Sladkova, "Radiation Levels in InterplanetaryPrognoz Satellites," Cosmic Radiation, 12_, No. 5, 716-718 (0. ArchamDeau, Mammalian Radiation Lethargy, A Disturbance

Lyman, J.T.

2010-01-01T23:59:59.000Z

355

Regulation of Annexin A2 by Ionizing Radiation in Human Skin...  

NLE Websites -- All DOE Office Websites (Extended Search)

of Annexin A2 by Ionizing Radiation in Human Skin Equivalent Culture: Does A Nuclear Annexin A2-Protein Kinase C Epsilon Complex Contribute To Reduced Cancer Risks At Low...

356

Low Dose Radiation Research Program: Radiation Alters Epithelial...  

NLE Websites -- All DOE Office Websites (Extended Search)

digital fluorescence microscopy. Typical integrin immunolocalization in mouse mammary gland is shown in Figure 2. text needed Figure 3 Relative immunoreactivity of a6 integrin...

357

Low Dose Radiation Program: Links - Radiation Research Related...  

NLE Websites -- All DOE Office Websites (Extended Search)

Research Related Databases Comprehensive Epidemiologic Data Resource (CEDR) U.S. Department of Energy (DOE) The European Radiobiology Archives Genomics: GTL - Systems Biology for...

358

Low Dose Radiation Research Program: DOE Lowdose Radiation Program...  

NLE Websites -- All DOE Office Websites (Extended Search)

inside the nuclear volume (5 m radius in our model) or in close proximity to chromatin fibers. As a stating point in these calculations we have considered six OH...

359

Radiation aging of stockpile and space-based microelectronics.  

SciTech Connect

This report describes an LDRD-supported experimental-theoretical collaboration on the enhanced low-dose-rate sensitivity (ELDRS) problem. The experimental work led to a method for elimination of ELDRS, and the theoretical work led to a suite of bimolecular mechanisms that explain ELDRS and is in good agreement with various ELDRS experiments. The model shows that the radiation effects are linear in the limit of very low dose rates. In this limit, the regime of most concern, the model provides a good estimate of the worst-case effects of low dose rate ionizing radiation.

Hembree, Charles Edward; Hjalmarson, Harold Paul

2004-02-01T23:59:59.000Z

360

Radiation-induced attenuation of high-OH optical fibers after hydrogen treatment in the presence of ionizing radiation  

DOE Green Energy (OSTI)

High purity, high-OH, optical fibers were irradiated in a hydrogen atmosphere to explore hydrogen binding into defects created by the ionizing radiation. Significant improvements in subsequent measurements of radiation-induced attenuation were observed. 18 refs., 4 figs., 2 tabs.

Lyons, P.B; Looney, L.D.

1991-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
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to obtain the most current and comprehensive results.


361

Use of ionizing radiation for fixing textile resins on wool. [Gamma rays  

SciTech Connect

Ambient-temperature treatments with ionizing radiation can be used as an alternative to conventional thermal/catalytic cure methods of fixing textile resins on wool materials. The effectiveness of the radiation-induced fixation of resins on wool has been demonstrated by machine-wash shrinkage tests on fabrics treated with a variety of commercial polymer resins.

McLaren, K.G.

1980-04-01T23:59:59.000Z

362

Low doses of neutrons induce changes in gene expression  

SciTech Connect

Studies were designed to identify genes induced in fibroblasts after exposure to low-dose neutron radiation but not after {gamma} rays. Our past work had shown similar modulation of transcripts for {alpha}-tubulin, {beta}- and {gamma}-actins, ornithine decarboxylase and interleukin 1 after exposure to either neutrons or {gamma} rays. However, differences in the expression of {beta}-protein kinase C and c-fos genes were observed, with both being induced after exposure to {gamma} rays but not neutrons. Recently, we have identified two genes that are induced after exposure to neutrons but not {gamma} rays: Rp-8 (a gene associated with apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency virus (HIV). Induction of Rp-8 mRNA was demonstrated in Syrian hamster embryo (SHE) fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.005 Gy/min) and high dose rate (0.12 Gy/min). No induction of other genes associated with apoptosis such as Rp-2, bcl-2 and Tcl-30 was observed. The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measurements of CAT activity and CAT transcripts after irradiation demonstrated an unresponsiveness to {gamma} rays over a broad range of doses (0.1-3 Gy). Twofold induction of the HIV-LTR was detected after exposure to neutrons (0.48 Gy) administered at low (0.05 Gy/min) but not high (0.12 Gy/min) dose rates. Ultraviolet-mediated HIV-LTR induction, however, was inhibited by exposure to low-dose-rate neutron irradiation. These results are interesting in light of reports that Rp-8 is induced during apoptosis and that HIV causes apoptosis. 17 refs., 3 figs., 1 tab.

Woloschak, G.E.; Chang-Liu, C.M. [Argonne National Lab., IL (United States); Panozzo, J.; Libertin, C.R. [Loyola Univ. of Chicago, Maywood, IL (United States)

1994-04-01T23:59:59.000Z

363

Consequences of low dose irradiation in the CNS  

NLE Websites -- All DOE Office Websites (Extended Search)

Consequences of low dose irradiation in the CNS Bertrand Tseng, Munjal M. Acharya, Neal Patel, Katherine Tran, Mary Lan, Erich Giedzinski, Vipan Kumar and Charles Limoli Department...

364

NIST Ionizing Radiat. Div. - 2004: Strategic Focus 3  

Science Conference Proceedings (OSTI)

... of high levels of absorbed dose, as required in the industrial radiation processing of ... tremendous increase in the use of low-energy, photon ...

365

NIST Ionizing Radiat. Div. - 2002: Strategic Focus 3  

Science Conference Proceedings (OSTI)

... of high levels of absorbed dose, as required in the industrial radiation processing of ... tremendous increase in the use of low-energy, photon ...

366

NIST Ionizing Radiat. Div. - 2004: Strategic Focus 2  

Science Conference Proceedings (OSTI)

... electronic radiation monitors ("pagers"), isotope identifier instruments ... 2003, DOE recognized this program as one of the top-ten programs relevant to ...

367

Does nitrate deposition following astrophysical ionizing radiation events pose an additional threat to amphibians?  

E-Print Network (OSTI)

It is known that amphibians are especially susceptible to the combination of heightened UVB radiation and increased nitrate concentrations. Various astrophysical events have been suggested as sources of ionizing radiation that could pose a threat to life on Earth, through destruction of the ozone layer and subsequent increase in UVB, followed by deposition of nitrate. In this study, we investigate whether the nitrate deposition following an ionizing event is sufficiently large to cause an additional stress beyond that of the heightened UVB previously considered. We have converted predicted nitrate depositions to concentration values, utilizing data from the New York State Department of Environmental Conservation Acid Rain Monitoring Network web site. Our results show that the increase in nitrate concentration in bodies of water following the most intense ionization event likely in the last billion years would not be sufficient to cause a serious additional stress on amphibian populations and may actually provide some benefit by acting as fertilizer.

Brian C. Thomas; Michelle D. Honeyman

2008-04-22T23:59:59.000Z

368

Amphibian nitrate stress as an additional terrestrial threat from astrophysical ionizing radiation events?  

E-Print Network (OSTI)

As diversity in amphibian species declines, the search for causes has intensified. Work in this area has shown that amphibians are especially susceptible to the combination of heightened UVB radiation and increased nitrate concentrations. Various astrophysical events have been suggested as sources of ionizing radiation that could pose a threat to life on Earth, through destruction of the ozone layer and subsequent increase in UVB, followed by deposition of nitrate. In this study, we investigate whether the nitrate deposition following an ionizing event is sufficiently large to cause an additional stress beyond that of the heightened UVB previously considered. We have converted predicted nitrate depositions to concentration values, utilizing data from the New York State Department of Environmental Conservation Acid Rain Monitoring Network web site. Our results show that the increase in nitrate concentration in bodies of water following the most intense ionization event likely in the last billion years would no...

Thomas, Brian C

2008-01-01T23:59:59.000Z

369

Detection of Ionizing Radiation by Plasma-Panel Sensors: Cosmic Muons, Ion Beams, and Cancer Therapy  

Science Conference Proceedings (OSTI)

The plasma panel sensor is an ionizing photon and particle radiation detector derived from PDP technology with high gain and nanosecond response. Experimental results in detecting cosmic ray muons and beta particles from radioactive sources are described along with applications including high energy and nuclear physics, homeland security and cancer therapeutics.

Friedman, Dr. Peter S. [Integrated Sensors, LLC; Ferretti, Claudio [University of Michigan; Ball, Robert [University of Michigan; Beene, James R [ORNL; Ben Moshe, M. [Tel Aviv University; Benhammou, Yan [Tel Aviv University; Chapman, J. Wehrley [University of Michigan; Levin, Daniel S. [University of Michigan; Silver, Yiftah [Tel Aviv University; Weaverdyck, Curtis [University of Michigan; Zhou, Bing [University of Michigan; Etzion, E [Tel Aviv University; Moshe, M. [Tel Aviv University; Bentefour, E [Ion Beam Applications

2012-01-01T23:59:59.000Z

370

The effects of diet and ionizing radiation on azoxymethane induced colon carcinogenesis  

E-Print Network (OSTI)

The ability of ionizing radiation to enhance colon carcinogenesis and the role of diet in this process has not been documented. We hypothesized that radiation would enhance the formation of aberrant crypt foci, ACF, known precursor lesions to colon cancer, by suppressing apoptosis and upregulating proliferation in colonocytes. Diets contained a combination of fish oil or corn oil and either pectin or cellulose. We exposed 40 male Sprague-Dawley rats to 1 Gy ionizing radiation (1 GeV Fe) 10 d prior to injection with AOM. Colons were resected at the promotion stage of carcinogenesis (7 wk post initial injection) and assayed for ACF and apoptosis. Radiation treatment increased (P=0.0327) the incidence of high multiplicity ACF (foci with four or more aberrant crypts) and decreased (P=0.0340) the apoptotic index compared to non-irradiated rats. Radiation also resulted in an increase (PACF compared to the corn oil treatment. Dietary pectin significantly increased (P=0.0204) the apoptotic index compared to cellulose treatment. These data suggest that ionizing radiation can work synergistically with AOM and increase the formation of high-multiplicity ACF, upregulate cellular proliferation and decrease apoptosis in colonocytes. The data also suggest that diets containing fish oil and pectin may protect against colon cancer by increasing apoptosis and reducing the formation of high multiplicity ACF.

Mann, John Clifford

2005-08-01T23:59:59.000Z

371

Low-dose Photons Modify CD4+ T Cell Signaling Response to Simulated Solar  

NLE Websites -- All DOE Office Websites (Extended Search)

Photons Modify CD4+ T Cell Signaling Response to Simulated Solar Photons Modify CD4+ T Cell Signaling Response to Simulated Solar Particle Event Protons Daila Gridley Loma Linda University and Medical Center Abstract Purpose: Astronauts on missions are exposed to low-dose/low-dose (LDR) radiation and could receive high doses during solar particle events (SPE). This study investigated T cell function in response to LDR radiation and simulated SPE (sSPE) protons, alone and in combination. Materials and methods: C57BL/6 mice received LDR γ-radiation (57Co) to a total dose of 0.01 Gray (Gy) at 0.0179 cGy/h, either with or without subsequent exposure to 1.7 Gy simulated SPE (sSPE) protons delivered over 36 h. On days 4 and 21 post-exposure, three functional pathways were studied using negatively isolated/anti-CD3 activated splenic CD4+ T cells:

372

Methylation of the ATM promoter in glioma cells alters ionizing radiation sensitivity  

SciTech Connect

Glioblastomas are among the malignancies most resistant to radiation therapy. In contrast, cells lacking the ATM protein are highly sensitive to ionizing radiation. The relationship between ATM protein expression and radiosensitivity in 3 glioma cell lines was examined. T98G cells exhibited normal levels of ATM protein, whereas U118 and U87 cells had significantly lower levels of ATM and increased (>2-fold) sensitivity to ionizing radiation compared to T98G cells. The ATM promoter was methylated in U87 cells. Demethylation by azacytidine treatment increased ATM protein levels in the U87 cells and decreased their radiosensitivity. In contrast, the ATM promoter in U118 cells was not methylated. Further, expression of exogenous ATM did not significantly alter the radiosensitivity of U118 cells. ATM expression is therefore heterogeneous in the glioma cells examined. In conclusion, methylation of the ATM promoter may account for the variable radiosensitivity and heterogeneous ATM expression in a fraction of glioma cells.

Roy, Kanaklata [Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Harvard Medical School, Dana-Farber Cancer Institute, 44 Binney St, Boston, MA 02115 (United States); Wang, Lilin [Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Harvard Medical School, Dana-Farber Cancer Institute, 44 Binney St, Boston, MA 02115 (United States); Makrigiorgos, G. Mike [Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Harvard Medical School, Dana-Farber Cancer Institute, 44 Binney St, Boston, MA 02115 (United States); Price, Brendan D. [Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Harvard Medical School, Dana-Farber Cancer Institute, 44 Binney St, Boston, MA 02115 (United States)]. E-mail: brendan_price@dfci.harvard.edu

2006-06-09T23:59:59.000Z

373

15TH INTERNATIONAL SYMPOSIUM ON TOXICITY ASSESSMENT Alpha radiation exposure decreases apoptotic cells in zebrafish  

E-Print Network (OSTI)

15TH INTERNATIONAL SYMPOSIUM ON TOXICITY ASSESSMENT Alpha radiation exposure decreases apoptotic of an adaptive response by the ionizing radiation against sub- sequent exposures to Cd. Keywords Multiple embryos subjected to a priming exposure provided by one environmental stressor (low-dose alpha particles

Yu, K.N.

374

Quantitative Phosphoproteomics Identifies Filaggrin and other Targets of Ionizing Radiation in a Human Skin Model  

Science Conference Proceedings (OSTI)

Our objective here was to perform a quantitative phosphoproteomic study on a reconstituted human skin tissue to identify low and high dose ionizing radiation dependent signaling in a complex 3-dimensional setting. Application of an isobaric labeling strategy using sham and 3 radiation doses (3, 10, 200 cGy) resulted in the identification of 1113 unique phosphopeptides. Statistical analyses identified 151 phosphopeptides showing significant changes in response to radiation and radiation dose. Proteins responsible for maintaining skin structural integrity including keratins and desmosomal proteins (desmoglein, desmoplakin, plakophilin 1 and 2,) had altered phosphorylation levels following exposure to both low and high doses of radiation. A phosphorylation site present in multiple copies in the linker regions of human profilaggrin underwent the largest fold change. Increased phosphorylation of these sites coincided with altered profilaggrin processing suggesting a role for linker phosphorylation in human profilaggrin regulation. These studies demonstrate that the reconstituted human skin system undergoes a coordinated response to ionizing radiation involving multiple layers of the stratified epithelium that serve to maintain skin barrier functions and minimize the damaging consequences of radiation exposure.

Yang, Feng; Waters, Katrina M.; Webb-Robertson, Bobbie-Jo M.; Sowa, Marianne B.; Freiin von Neubeck, Claere H.; Aldrich, Joshua T.; Markillie, Lye Meng; Wirgau, Rachel M.; Gristenko, Marina A.; Zhao, Rui; Camp, David G.; Smith, Richard D.; Stenoien, David L.

2012-04-17T23:59:59.000Z

375

Low Dose Radiation Research Program: Molecular Energetics of Clustered  

NLE Websites -- All DOE Office Websites (Extended Search)

Molecular Energetics of Clustered Damage Sites Molecular Energetics of Clustered Damage Sites Authors and Institutions: Principal Investigator: Dr. Michel Dupuis (PNNL) Co-investigators: Professor John H. Miller (WSU Tri-Cities), Professor Robert D. Stewart (Purdue University), Dr. Maciej S. Gutowski (PNNL), Dr. Eric J. Ackerman (PNNL); Collaborators: Mr. Matt Hernst (WSU Tri-Cities), Dr. Vladimir A. Semenenko (Purdue University), Mr. Maciej Haranczyk (Gdansk University , Poland), Mr. Rafal A. Bachorz (Poznan University, Poland), and Ms. Iwona Dabkowska (Gdansk University, Poland). Project: The goal of this project is to provide critical information to help characterize clustered damage sites relative to singly damaged sites with respect to their susceptibility to DNA repair. The premise is that differences in base pairing rules and mutagenic properties of singly and

376

Low Dose Radiation Cancer Risks: Epidemiological and Toxicological Models  

Science Conference Proceedings (OSTI)

The basic purpose of this one year research grant was to extend the two stage clonal expansion model (TSCE) of carcinogenesis to exposures other than the usual single acute exposure. The two-stage clonal expansion model of carcinogenesis incorporates the biological process of carcinogenesis, which involves two mutations and the clonal proliferation of the intermediate cells, in a stochastic, mathematical way. The current TSCE model serves a general purpose of acute exposure models but requires numerical computation of both the survival and hazard functions. The primary objective of this research project was to develop the analytical expressions for the survival function and the hazard function of the occurrence of the first cancer cell for acute, continuous and multiple exposure cases within the framework of the piece-wise constant parameter two-stage clonal expansion model of carcinogenesis. For acute exposure and multiple exposures of acute series, it is either only allowed to have the first mutation rate vary with the dose, or to have all the parameters be dose dependent; for multiple exposures of continuous exposures, all the parameters are allowed to vary with the dose. With these analytical functions, it becomes easy to evaluate the risks of cancer and allows one to deal with the various exposure patterns in cancer risk assessment. A second objective was to apply the TSCE model with varing continuous exposures from the cancer studies of inhaled plutonium in beagle dogs. Using step functions to estimate the retention functions of the pulmonary exposure of plutonium the multiple exposure versions of the TSCE model was to be used to estimate the beagle dog lung cancer risks. The mathematical equations of the multiple exposure versions of the TSCE model were developed. A draft manuscript which is attached provides the results of this mathematical work. The application work using the beagle dog data from plutonium exposure has not been completed due to the fact that the research project did not continue beyond its first year.

David G. Hoel, PhD

2012-04-19T23:59:59.000Z

377

Low Dose Radiation Research Program: Frequently Asked Questions  

NLE Websites -- All DOE Office Websites (Extended Search)

between these exposures and an increased cancer risk. National Academy of Sciences Report, Possible health effects of residential electric and magnetic fields. Return to top...

378

Low Dose Radiation Research Program: Multi-cellular Crosstalk...  

NLE Websites -- All DOE Office Websites (Extended Search)

grown in microwell slide chambers will be irradiated with precise 100-mm-wide exposure stripes of dose to define the responses in exposed and bystander cells The time course of the...

379

Low Dose Radiation Research Program: X-ray Microbeam Bystander...  

NLE Websites -- All DOE Office Websites (Extended Search)

experiments, cultures grown in microwell slide chambers were irradiated with precise stripes of dose up to 100m wide. Samples were processed for the expression of...

380

Low Dose Radiation Research Program: Funded Project Descriptions  

NLE Websites -- All DOE Office Websites (Extended Search)

Funded Project Descriptions Funded Project Descriptions Non-Invasive Early Detection and Molecular Analysis of Low X-Ray Dose Effects in the Lens Jointly funded by NASA and DOE Principal Investigator: Lee Goldstein, M.D., Ph.D., Associate Professor in Psychiatry, Neurology, Ophthalmology, Pathology and Laboratory Medicine, & Biomedical Engineering, Boston University’s School Medicine, College of Engineering, and Photonics Center. Boston, Ma. The project includes a new DOE FWP (~$400 K over 3 years) to Lawrence Berkeley National Laboratory with Eleanor Blakely as Project Leader. The work includes a subcontract to support the collaboration of Polly Chang of SRI, International, Menlo Park, CA. and is scheduled to begin as early as August 2009. This proposal was submitted in response to the joint DOE/NASA

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381

Low Dose Radiation Research Program: Mating-Type Regulation of  

NLE Websites -- All DOE Office Websites (Extended Search)

Mating-Type Regulation of Non-homologous End Joining in Mating-Type Regulation of Non-homologous End Joining in Saccharomyces cerevisiae Authors: Maria Valencia,* Marc Bentele,^ Moreshwar B. Vaze,* Gernot Herrmann, Ellayhu Kraus, Sang Eun Lee, Primo Schar,^ and James E. Haber* Institutions: *Rosenstiel Center and Department of Biology, Brandeis University ^Institute of Medical Radiobiology, University of Zurich, Zurich, Switzerland. In the budding yeast, Saccharomyces cerevisiae, broken DNA ends can be ligated, either in a precise or an error-prone manner by a process known as Non-Homologous End Joining (NHEJ). Several proteins have been identified that play a role in NHEJ: the yKu70/80 heterodimer, DNA ligase IV and its associated protein Lif1/Xrcc4, the Mre11-Rad50-Xrs2 complex, as well as the silencing proteins Sir 2, 3, 4. Recent studies suggest that SIR genes may

382

Global methylation responses to low dose radiation exposure  

NLE Websites -- All DOE Office Websites (Extended Search)

comprises a very large proportion of the mouse and human genomes. Using bisulphite modification and quantitative real-time PCR, the method can be used to analyse a very large pool...

383

Low Dose Radiation Research Program: Richard D. Smith  

NLE Websites -- All DOE Office Websites (Extended Search)

W.J., Wang, H.X., Petyuk, V.A., Bloom, J.S., Sforza, D.M., Lacan, G., Liu, D., Khan, A.H., Cantor, R.M., Bigelow, D.J., Melega, W.P., Camp, D.G., Smith, R.D., and Smith, D.J....

384

Low Dose Radiation Research Program: Real-Time Molecular Study...  

NLE Websites -- All DOE Office Websites (Extended Search)

Real-Time Molecular Study of Bystander Effects Using Imaging and Nano-Particle Optics Mohan Natarajan University of Texas Health Science Center Why this Project? To develop...

385

Low Dose Radiation Research Program: Mechanistic Modeling of...  

NLE Websites -- All DOE Office Websites (Extended Search)

Texas A&M University College Station TX 77843 The primary objective of this project is to provide data for building a mathematical model of the role of repair in the...

386

Low Dose Radiation Research Program: Mary Helen Barcellos-Hoff  

NLE Websites -- All DOE Office Websites (Extended Search)

B.B. and Barcellos-Hoff, M.H. (2001) Epigenetics and breast cancer. Journal of Mammary Gland Biology Neoplasia 6(2):151-152. Barcellos-Hoff, M.H. and Brooks, A.L. (2001)....

387

MECHANISTIC STUDY OF LOW DOSE RADIATION INDUCED PROTEOLYTIC CASCADES  

NLE Websites -- All DOE Office Websites (Extended Search)

Cells organize into tissue-like structures which recapitulate the intact human mammary gland morphology (Figure 1) enabling molecular level study of how normal tissues respond...

388

Nanoprobes for Imaging Single Cells under Low-Dose Radiation  

NLE Websites -- All DOE Office Websites (Extended Search)

Oak Ridge National Laboratory, Oak Ridge, TN 37831 2 Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708 3 Student Intern at Oak Ridge National Laboratory...

389

Low Dose Radiation Research Program: Oxidative Stress Signaling...  

NLE Websites -- All DOE Office Websites (Extended Search)

Medical Research Council, Harwell, Oxford, United Kingdom. Cells initially surviving irradiation and capable of proliferation may produce descendants with de-nova chromosome...

390

Low Dose Radiation Research Program: Comparison of Soft X Rays...  

NLE Websites -- All DOE Office Websites (Extended Search)

Comparison of Soft X-Rays and Ions Irradiation in a Model of V79 Mammalian Cell Authors: B. Ginovska, J.H. Miller, D. J. Lynch and W. E. Wilson Institutions: School of Electrical...

391

Low Dose Radiation Research Program: Induction of Genomic Instability...  

NLE Websites -- All DOE Office Websites (Extended Search)

genetic backgrounds (BALBcJ and C57BL6J mice) collected at different times post-irradiation (i.e. 1 hr, 4 hrs, 1 month and 6 months). A total of five mice per dose per strain...

392

Low Dose Radiation Research Program: John R. Ford  

NLE Websites -- All DOE Office Websites (Extended Search)

2002 Workshop: Biological Response of Individual Cells Following Electron Microbeam Irradiation Braby, L.A. and Ford, J.R., Texas A&M University, College Station TX Publications...

393

Low Dose Radiation Research Program: Induction of Genomic Instability...  

NLE Websites -- All DOE Office Websites (Extended Search)

lesions in the progeny of exposed cells for several cell generations following irradiation. It has been hypothesized that genomic instability is a key event in the...

394

Low Dose Radiation Research Program: Genetic Factors affecting...  

NLE Websites -- All DOE Office Websites (Extended Search)

on the genetic background of the cells examined and NBS cells do not form foci after irradiation. In preliminary experiments using IMR90 human fibroblasts, formation of foci...

395

Low Dose Radiation Research Program: Barry D. Michael  

NLE Websites -- All DOE Office Websites (Extended Search)

B.D. (2006). Bystander-induced differentiation: A major response to targeted irradiation of a urothelial explant model. Mutation Research 597(1-2):43-49. Prise, K.M.,...

396

Low Dose Radiation Research Program: Leslie A. Braby - Mechanistic...  

NLE Websites -- All DOE Office Websites (Extended Search)

J.R. 2002 Workshop: Characterizing Bystander Effects Following Electron Microbeam Irradiation. Braby, L.A. and Ford, J.R. 2001 Workshop: Biological Response of Individual Cells...

397

Any apoptotic bystander effect induced by low dose radiation...  

NLE Websites -- All DOE Office Websites (Extended Search)

apoptosis frequency in situ in spleen tissue sections at various time-points after irradiation and compared the apoptosis frequency with that predicted from LNT. This approach...

398

Low Dose Radiation Stimulates Antioxidant Capacity in the Brain...  

NLE Websites -- All DOE Office Websites (Extended Search)

Mu , C-M Charlie Ma , Lili Chen , Darrell Q. Brown , Sam Litwin , and Alfred G. Knudson Fox Chase Cancer Center, Philadelphia, PA Brian J. Augelli , S. Ausim Azizi , and Barbara...

399

Low Dose Radiation Research Program: 2010 Current Projects  

NLE Websites -- All DOE Office Websites (Extended Search)

University Automated Data Sharing between the Janus and ERA radiobiology archives Hlatky, Lynn Tufts University School of Medicine Evidence for a Non-Linear Dependence of Tumor...

400

A Systems Genetics Approach to Low Dose Radiation  

NLE Websites -- All DOE Office Websites (Extended Search)

of Genome Science and Technology, University of Tennessee, Knoxville; 3 Department of Electrical Engineering and Computer Science, University of Tennessee, Knoxville ; 4...

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
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to obtain the most current and comprehensive results.


401

"Genetic Factors Affecting Susceptibility to Low-Dose Radiation...  

NLE Websites -- All DOE Office Websites (Extended Search)

Laboratory, University of Maryland, Baltimore, MD 2 Virginia Mason Cancer Center, Seattle, WA 3 Memorial Sloan Kettering Cancer Center, New York, NY Rationale: Risk estimates...

402

Low Dose Radiation Research Program: An Expression Array Strategy...  

NLE Websites -- All DOE Office Websites (Extended Search)

Mouse Strain Specific DNA Damage Response Pathways in Mammary Tissue Allan Balmain Cancer Research Institute, University of California at San Francisco, San Francisco CA...

403

Low Dose Radiation Research Program: Impact of Genetic Factors...  

NLE Websites -- All DOE Office Websites (Extended Search)

following paternal F0 137Cs gamma irradiation with doses of 1.0 Gy in CD1 mice. Pilot studies demonstrate effects in at least the F1 generation following paternal F0...

404

Low Dose Radiation Research Program: Modeling the Physics of...  

NLE Websites -- All DOE Office Websites (Extended Search)

spatial patterns of damage produced. This project focuses on refining the physics of electron transport in a heterogeneous cellular medium. We intend to expand current...

405

Low Dose Radiation Research Program: Modeling Energy Deposition...  

NLE Websites -- All DOE Office Websites (Extended Search)

to accurately simulate the production of damage clusters in the cellular medium. Electron interaction cross-sections used in MC codes are generally derived from "high-energy"...

406

Low Dose Radiation Research Program: Modeling Energy Deposition...  

NLE Websites -- All DOE Office Websites (Extended Search)

physical parameters employed in these codes faces even greater challenges. Electron interaction crosssections used in MC codes are generally derived from "high-energy"...

407

Low Dose Radiation Research Program: Spatially Resolved Single...  

NLE Websites -- All DOE Office Websites (Extended Search)

a biological irradiation chamber mounted on an X-Y-Z scanning stage of a standard optical microscope. Cells are plated on a 1.5-m mylar membrane that is placed directly on the...

408

Low Dose Radiation Research Program: A Model for Interphase Chromosome...  

NLE Websites -- All DOE Office Websites (Extended Search)

the 46 interphase chromosomes is modeled as a random polymer involving 30 nm zig-zag chromatin fiber confined within a sphere whose radius is dependent upon the chromosome length...

409

Low Dose Radiation Research Program: Occurrence of a Positive...  

NLE Websites -- All DOE Office Websites (Extended Search)

electrophoretic mobility shift assay (EMSA), (b) the kinetics of TNF-a expression by RT-PCR (mRNA expression) and ELISA (secreted protein expression), and (c) the involvement of...

410

Low Dose Radiation Research Program: The Adaptive Response in...  

NLE Websites -- All DOE Office Websites (Extended Search)

Dolling,1 Ron E Mitchel,2 and Douglas R Boreham1 Institutions: 1McMaster University, Canada,2 Atomic Energy of Canada Limited, Canada The Trp53 gene is clearly associated with...

411

Low dose and bystander responses in a 3-D human skin model  

NLE Websites -- All DOE Office Websites (Extended Search)

and bystander responses in a 3-D human skin model and bystander responses in a 3-D human skin model Sally A. Amundson Columbia University Medical Center Abstract Significant structural abnormalities develop within several days of exposure of the 3-dimensional normal human skin tissue model EPI-200 (MatTek) to high or low doses of low LET radiation. Disruption of the basal layer occurs following high radiation doses, and premature cornification is evident after both high and low dose exposures. In bystander tissue that is near irradiated portions of the tissue, but is not itself irradiated, we also observe premature cornification, increased apoptosis and micronucleus formation. Changes in global gene expression also occur in both directly irradiated and bystander EPI-200 tissue. Although the unfolding over time

412

Low dose and bystander responses in a 3-D human skin model  

NLE Websites -- All DOE Office Websites (Extended Search)

and bystander responses in a 3-D human skin model. and bystander responses in a 3-D human skin model. Sally A. Amundson and Alexandre Mezentsev Columbia University Medical Center, Center for Radiological Research, New York, NY 10032 Significant structural abnormalities develop within several days of exposure of the 3-dimensional normal human skin tissue model EPI-200 (MatTek) to high or low doses of low LET radiation. Disruption of the basal layer occurs following high radiation doses, and premature cornification is evident after both high and low dose exposures. In bystander tissue that is near irradiated portions of the tissue, but is not itself irradiated, we also observe premature cornification, increased apoptosis and micronucleus formation. Changes in global gene expression also occur

413

Cosmological constraints in the presence of ionizing and resonance radiation at recombination  

E-Print Network (OSTI)

With the recent measurement of full sky cosmic microwave background polarization from WMAP, key cosmological degeneracies have been broken, allowing tighter constraints to be placed on cosmological parameters inferred assuming a standard recombination scenario. Here we consider the effect on cosmological constraints if additional ionizing and resonance radiation sources are present at recombination. We find that the new CMB data significantly improve the constraints on the additional radiation sources, with $\\log_{10}[\\epsilon_{\\alpha}] < -0.5$ and $\\log_{10}[\\epsilon_{i}] <-2.4$ at 95% c.l. for resonance and ionizing sources respectively. Including the generalized recombination scenario, however, we find that the constraints on the scalar spectral index $n_s$ are weakened to $n_s=0.98\\pm0.03$, with the $n_s=1$ case now well inside the 95% c.l.. The relaxation of constraints on tensor modes, scale invariance, dark energy and neutrino masses are also discussed.

Rachel Bean; Alessandro Melchiorri; Joe Silk

2007-01-09T23:59:59.000Z

414

Technical specifications manual for the MARK-1 pulsed ionizing radiation detection system. Volume 1  

Science Conference Proceedings (OSTI)

The MARK-1 detection system was developed by the Idaho National Engineering Laboratory for the US Department of Energy Office of Arms Control and Nonproliferation. The completely portable system was designed for the detection and analysis of intense photon emissions from pulsed ionizing radiation sources. This manual presents the technical design specifications for the MARK-1 detection system and was written primarily to assist the support or service technician in the service, calibration, and repair of the system. The manual presents the general detection system theory, the MARK-1 component design specifications, the acquisition and control software, the data processing sequence, and the system calibration procedure. A second manual entitled: Volume 2: Operations Manual for the MARK-1 Pulsed Ionizing Radiation Detection System (USDOE Report WINCO-1108, September 1992) provides a general operational description of the MARK-1 detection system. The Operations Manual was written primarily to assist the field operator in system operations and analysis of the data.

Lawrence, R.S.; Harker, Y.D.; Jones, J.L.; Hoggan, J.M.

1993-03-01T23:59:59.000Z

415

Exposure to ionizing radiation in the emergency department from commonly performed portable radiographs  

SciTech Connect

To accurately assess the potential hazard of exposure to ionizing radiation from portable radiographs taken in the emergency department, a study was performed to measure such radiation at different distances from the edge of an irradiated field during portable cervical-spine (pC-S), portable chest radiograph (pCXR), and portable anteroposterior-pelvis (pAP-pelvis) radiographs. For all three types of portable radiographs, radiation exposure is a function of distance from the beam. However, at 40 cm (15 inches) away from the beam during a pC-S or pCXR and at 160 cm (63 inches) from a pAP-pelvis film, exposure is minimal. At these distances one would need to be exposed to more than 1,200 such radiographs to equal background environmental ionizing radiation. Medical personnel should not have to leave a patient care area for fear of undue acute and chronic radiation exposure while portable radiographs are performed in the ED. By using protective garments and standing appropriate distances away from the patient, continuous patient care can be maintained while portable radiographs are taken in the ED.

Grazer, R.E.; Meislin, H.W.; Westerman, B.R.; Criss, E.A.

1987-04-01T23:59:59.000Z

416

Effects of Ionizing Radiation on Digital Single Event Transients in a 180-nm Fully Depleted SOI Process  

E-Print Network (OSTI)

Effects of ionizing radiation on single event transients are reported for Fully Depleted SOI (FDSOI) technology using experiments and simulations. Logic circuits, i.e. CMOS inverter chains, were irradiated with cobalt-60 ...

Keast, Craig L.

417

POSITION STATEMENT DOCUMENTING IONIZING RADIATION EXPOSURE IN THE ELECTRONIC HEALTH RECORD  

E-Print Network (OSTI)

IEEE-USA believes that maintaining an accurate record of a patients cumulative exposure to ionizing radiation can be of substantial value for clinical, health services management and research purposes. Excessive radiation can cause cancer, skin and bone marrow disease and a variety of other diseases. Radiation exposure can come from a number of sources, both natural and manmade. Over the lifetime of the individual, medical procedures, such as X-rays, Computed Tomography (CT) scans, and isotope radiation therapy, contribute significant radiation dosages. Monitoring the use of radiological procedures, measuring the radiation dose directly, and recording the dose in the Electronic Health Record (EHR) can help assess and reduce the risk posed by excessive radiation while optimizing the diagnostic value of these procedures. With these risks and benefits in mind, IEEE-USA recommends the following: 1. Health Level Seven International (HL7), the global authority responsible for standards for interoperability of health information technology, should add the appropriate "dose object " radiation parameters, as defined in the IHE Radiology Technical Framework Supplement, Radiation Exposure Monitoring (REM), to HL7/CDA format longitudinal electronic health records (EHRs), to create a longitudinal record of a patient's exposure to radiation resulting from medical procedures. These records should note both the procedure and estimated radiation dose from each procedure. 2. The Office of the National Coordinator for Health Information Technology (ONCHIT) should make radiation dose monitoring a part of meaningful use criteria for EHRs. 3. Healthcare accreditation organizations (JCAHO, NCQA and URAC), in collaboration with the American College of Radiologists (ACR), should continue

Adopted The Ieee-usa

2011-01-01T23:59:59.000Z

418

New Computational Methods for Characterizing Systems Biology of Low Dose  

NLE Websites -- All DOE Office Websites (Extended Search)

New Computational Methods for Characterizing Systems Biology of Low Dose New Computational Methods for Characterizing Systems Biology of Low Dose and Adaptive Response Bahram Parvin Lawrence Berkeley National Laboratory Abstract We present preliminary results on a new computational method for systems biology of adaptive response and low dose effect from transcript and phenotypic data. The underlying concept is that a small subset of genes is triggered for each treatment condition or a phenotypic index. The concept of a small subset of genes translates to the sparsity constraint, which is applied computationally. The main advantage of this technique over traditional statistical methods is (i) direct application of sparsity, (ii) incorporating multi-class and multidimensional phenotypic profiles in one framework, and (iii) hypothesizing interaction networks simultaneously. Our

419

RADIATION MONITOR CONTAINING TWO CONCENTRIC IONIZATION CHAMBERS AND MEANS FOR INSULATING THE SEPARATE CHAMBERS  

DOE Patents (OSTI)

This invention relates to a portable radiation monitor containing two concentric ionization chambers which permit the use of standard charging and reading devices. It is particularly adapted as a personnel x-ray dosimeter and to this end comprises a small thin walled, cylindrical conductor forming an inner energy dependent chamber, a small thin walled, cylindrical conductor forming an outer energy independent chamber, and polymeric insulation means which insulates said chambers from each other and holds the chambers together with exposed connections in a simple, trouble-free, and compact assembly substantially without variation in directional response. (AEC)

Braestrup, C.B.; Mooney, R.T.

1964-01-21T23:59:59.000Z

420

Custom Device for Low-Dose Gamma Irradiation of Biological Samples  

E-Print Network (OSTI)

When astronauts travel in space, their primary health hazards are high-energy cosmic radiations from galactic cosmic rays (GCR). Most galactic cosmic rays have energies between 100 MeV and 10 GeV. For occupants inside of a space shuttle, the structural material is efficient to absorb most of the cosmic-ray energy and reduce the interior dose rate to below 1.2 mGy per day. However, the biological effects of prolonged exposure to low-dose radiation are not well understood. The purpose of this research was to examine the feasibility of constructing a low-dose irradiation facility to simulate the uniform radiation field that exists in space. In this research, we used a pre-manufactured incubator, specifically the Thermo Scientific Forma Series II Water Jacketed CO2 Incubator, to act as shielding and simulate the exterior of the space shuttle. To achieve the desired dose rate (< 1 mGy/h) inside the incubator volume, the computer code MCNPX was used to determine required source activity and distance between the shielding and source. Once the activity and distance were calculated, an experiment was carried out to confirm the simulation results. The confirmation used survey meters and thermoluminescence dosimeters (TLDs) to map the radiation field within the incubator.

Bi, Ruoming

2011-12-01T23:59:59.000Z

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421

Alterations in transcription factor binding in radioresistant human melanoma cells after ionizing radiation  

Science Conference Proceedings (OSTI)

We analyzed alterations in transcription factor binding to specific, known promoter DNA consensus sequences between irradiated and unirradiated radioresistant human melanoma (U1-Mel) cells. The goal of this study was to begin to investigate which transcription factors and DNA-binding sites are responsible for the induction of specific transcripts and proteins after ionizing radiation. Transcription factor binding was observed using DNA band-shift assays and oligonucleotide competition analyses. Confluence-arrested U1-Mel cells were irradiated (4.5 Gy) and harvested at 4 h. Double-stranded oligonucleotides containing known DNA-binding consensus sites for specific transcription factors were used. Increased DNA binding activity after ionizing radiation was noted with oligonucleotides containing the CREB, NF-kB and Sp1 consensus sites. No changes in protein binding to AP-1, AP-2, AP-3, or CTF/NF1, GRE or Oct-1 consensus sequences were noted. X-ray activation of select transcription factors, which bind certain consensus sites in promoters, may cause specific induction or repression of gene transcription. 22 refs., 2 figs.

Sahijdak, W.M.; Yang, Chin-Rang; Zuckerman, J.S.; Meyers, M.; Boothman, D.A. [Univ. of Wisconsin, Madison, WI (United States)

1994-04-01T23:59:59.000Z

422

Examination system utilizing ionizing radiation and a flexible, miniature radiation detector probe  

DOE Patents (OSTI)

An optimized examination system and method based on the Reverse Geometry X-Ray.RTM. (RGX.RTM.) radiography technique are presented. The examination system comprises a radiation source, at least one flexible, miniature radiation detector probe positioned in appropriate proximity to the object to be examined and to the radiation source with the object located between the source and the probe, a photodetector device attachable to an end of the miniature radiation probe, and a control unit integrated with a display device connected to the photodetector device. The miniature radiation detector probe comprises a scintillation element, a flexible light guide having a first end optically coupled to the scintillation element and having a second end attachable to the photodetector device, and an opaque, environmentally-resistant sheath surrounding the flexible light guide. The probe may be portable and insertable, or may be fixed in place within the object to be examined. An enclosed, flexible, liquid light guide is also presented, which comprises a thin-walled flexible tube, a liquid, preferably mineral oil, contained within the tube, a scintillation element located at a first end of the tube, closures located at both ends of the tube, and an opaque, environmentally-resistant sheath surrounding the flexible tube. The examination system and method have applications in non-destructive material testing for voids, cracks, and corrosion, and may be used in areas containing hazardous materials. In addition, the system and method have applications for medical and dental imaging.

Majewski, Stanislaw (Grafton, VA); Kross, Brian J. (Yorktown, VA); Zorn, Carl J. (Yorktown, VA); Majewski, Lukasz A. (Grafton, VA)

1996-01-01T23:59:59.000Z

423

Examination system utilizing ionizing radiation and a flexible, miniature radiation detector probe  

DOE Patents (OSTI)

An optimized examination system and method based on the Reverse Geometry X-Ray{trademark} (RGX{trademark}) radiography technique are presented. The examination system comprises a radiation source, at least one flexible, miniature radiation detector probe positioned in appropriate proximity to the object to be examined and to the radiation source with the object located between the source and the probe, a photodetector device attachable to an end of the miniature radiation probe, and a control unit integrated with a display device connected to the photodetector device. The miniature radiation detector probe comprises a scintillation element, a flexible light guide having a first end optically coupled to the scintillation element and having a second end attachable to the photodetector device, and an opaque, environmentally-resistant sheath surrounding the flexible light guide. The probe may be portable and insertable, or may be fixed in place within the object to be examined. An enclosed, flexible, liquid light guide is also presented, which comprises a thin-walled flexible tube, a liquid, preferably mineral oil, contained within the tube, a scintillation element located at a first end of the tube, closures located at both ends of the tube, and an opaque, environmentally-resistant sheath surrounding the flexible tube. The examination system and method have applications in non-destructive material testing for voids, cracks, and corrosion, and may be used in areas containing hazardous materials. In addition, the system and method have applications for medical and dental imaging. 5 figs.

Majewski, S.; Kross, B.J.; Zorn, C.J.; Majewski, L.A.

1996-10-22T23:59:59.000Z

424

The carcinogenic risks of low-LET and high-LET ionizing radiations  

SciTech Connect

New information is available concerning the carcinogenic effects of radiation and the implications for risk assessment and risk management. This information comes from further follow-up of the epidemiological studies of the Japanese atomic bomb survivors, patients irradiated medically for cancer and allied conditions, and workers exposed in various occupations. In the Japanese atomic bomb survivors the carcinogenic risks are estimated to be somewhat higher than previously, due to the reassessment of the atomic-bomb dosimetry, further follow-up with increase in the number of excess cancer deaths, particularly in survivors irradiated early in life, and changes in the methods of analysis to compute the age-specific risks of cancer. Because of the characteristics of the atomic bomb survivor series as regards sample size, age and sex distribution, duration for follow-up, person-years at risk, and type of dosimetry, the mortality experience of the atomic bomb survivors was selected by the UNSCEAR Committee and the BEIR V Committee as the more appropriate basis for projecting risk estimates for the general population. In the atomic bomb survivors, the dose-effect relationship for overall cancer mortality other than leukemia is consistent with linearity below 3 Gy, while the dose-effect relationship for leukemia, excluding chronic lymphatic leukemia, conforms best to a linear-quadratic function. The shape of the dose-incidence curve at low doses still remains uncertain, and the data do not rule out the possible existence of a threshold for an neoplasm. The excess relative risk of mortality from all cancers combined is estimated to be 1.39 per Gy (shielded kerma), which corresponds to an absolute risk of 10.0 excess cancer deaths per 10,000 PYGy; the relative risks is 1.41 at 1 Gy (organ-absorbed dose), and an absolute risk of 13.07 excess cancer deaths per 10,000 PYGy. 19 refs.

Fabrikant, J.I. (Lawrence Berkeley Lab., CA (USA))

1989-08-01T23:59:59.000Z

425

Development and characterization of acrylated palm oil nanoparticles using ionizing radiation  

SciTech Connect

In this study, the utilization of radiation crosslinking methods which are known as intermolecular and intramolecular crosslinking for the formation of nanoparticles of Acrylated Palm Oil (APO) in the microemulsion system that also consists of Pluronic F-127 (PF-127) surfactant was demonstrated. This microemulsion system was subjected to the ionizing radiation i.e. gamma irradiation at different doses to form the crosslinked APO nanoparticles. The effects of radiation doses on the size of APO nanoparticles were investigated using the Dynamic Light Scattering (DLS) method and their images were viewed using the Transmission Electron Microcrospy (TEM). The Fourier Transform Infra-Red (FTIR) spectroscopy was used to characterize the chemical structure and the crosslinking conversion of carbon-carbon double bond (-C = C-) of the APO nanoparticles after irradiation. As a result, the size of the APO nanoparticle decreased when the irradiation dose increased. Reduce in size might be due to the effect of intramolecular crosslinking reaction of the APO nanoparticles during irradiation process. Meanwhile, the intramolecular -C C- crosslinking conversion percentage was increased at doses below 1kGy before decreasing at the higher dose that might due to the intermolecular crosslinking of the macromolecules. This study showed that radiation crosslinking methods of polymerization and crosslinking in the microemulsion were found to be promising for the synthesis of nanoparticles.

Tajau, Rida; Yunus, Wan Md Zin Wan; Dahlan, Khairul Zaman Mohd; Mahmood, Mohd Hilmi; Hashim, Kamaruddin [Malaysian Nuclear Agency (Nuclear Malaysia), Radiation Processing Technology Division (BTS), Bangi, 43000 Kajang, Selangor (Malaysia); Chemistry Department, Faculty of Science, University Putra Malaysia (UPM), 43400 UPM Serdang, Selangor (Malaysia); Malaysian Nuclear Agency (Nuclear Malaysia), Radiation Processing Technology Division (BTS), Bangi, 43000 Kajang, Selangor (Malaysia)

2012-11-27T23:59:59.000Z

426

The association betweeen cancers and low level radiation: An evaluation of the epidemiological evidence at the Hanford Nuclear Weapons Facility  

Science Conference Proceedings (OSTI)

Cancer has traditionally been linked to exposure to high doses of radiation, but there is considerable controversy regarding the carcinogenicity of low doses of ionizing radiation in humans. Over the past 30 years there have been 14 studies conducted on employees at the Hanford nuclear weapons facility to investigate the relationship between exposure to low doses of radiation and mortality due to cancer (1-14). Interest in this issue was originally stimulated by the Atomic Energy Commission (AEC) which was trying to determine whether the linear extrapolation of health effects from high to low dose exposure was accurate. If the risk has been underestimated, then the maximum permissible occupational radiation exposure in the United States had been set too high. Because the health risk associated with low level radiation are unclear and controversial it seems appropriate to review the studies relating to Hanford at this time.

Britton, J. [Univ. of California, Berkeley, CA (United States). School of Public Health]|[Lawrence Berkeley National Lab., CA (United States)

1993-05-01T23:59:59.000Z

427

Low-dose Photon and Simulated Solar Particle Event Proton Effects on Foxp3+  

NLE Websites -- All DOE Office Websites (Extended Search)

Photon and Simulated Solar Particle Event Proton Effects on Foxp3+ Photon and Simulated Solar Particle Event Proton Effects on Foxp3+ Treg Cells and Other Leukocytes Daila Gridley Loma Linda University and Medical Center Abstract Purpose: Radiation is a major factor in the spaceflight environment that can compromise immune defense mechanisms. Astronauts on missions are continuously exposed to lowdose/ low-dose-rate (LDR) radiation and may receive relatively high doses during a solar particle event (SPE) that consists primarily of protons. However, there are very few reports in which LDR photons were combined with protons. The goal of this study was to determine whether exposure to LDR γ-rays would modulate the effect of proton radiation mimicking an SPE. Materials and Methods: C57BL/6 mice were exposed to 1.7 Gy simulated SPE

428

Low-dose computed tomography image restoration using previous normal-dose scan  

Science Conference Proceedings (OSTI)

Purpose: In current computed tomography (CT) examinations, the associated x-ray radiation dose is of a significant concern to patients and operators. A simple and cost-effective means to perform the examinations is to lower the milliampere-seconds (mAs) or kVp parameter (or delivering less x-ray energy to the body) as low as reasonably achievable in data acquisition. However, lowering the mAs parameter will unavoidably increase data noise and the noise would propagate into the CT image if no adequate noise control is applied during image reconstruction. Since a normal-dose high diagnostic CT image scanned previously may be available in some clinical applications, such as CT perfusion imaging and CT angiography (CTA), this paper presents an innovative way to utilize the normal-dose scan as a priori information to induce signal restoration of the current low-dose CT image series. Methods: Unlike conventional local operations on neighboring image voxels, nonlocal means (NLM) algorithm utilizes the redundancy of information across the whole image. This paper adapts the NLM to utilize the redundancy of information in the previous normal-dose scan and further exploits ways to optimize the nonlocal weights for low-dose image restoration in the NLM framework. The resulting algorithm is called the previous normal-dose scan induced nonlocal means (ndiNLM). Because of the optimized nature of nonlocal weights calculation, the ndiNLM algorithm does not depend heavily on image registration between the current low-dose and the previous normal-dose CT scans. Furthermore, the smoothing parameter involved in the ndiNLM algorithm can be adaptively estimated based on the image noise relationship between the current low-dose and the previous normal-dose scanning protocols. Results: Qualitative and quantitative evaluations were carried out on a physical phantom as well as clinical abdominal and brain perfusion CT scans in terms of accuracy and resolution properties. The gain by the use of the previous normal-dose scan via the presented ndiNLM algorithm is noticeable as compared to a similar approach without using the previous normal-dose scan. Conclusions: For low-dose CT image restoration, the presented ndiNLM method is robust in preserving the spatial resolution and identifying the low-contrast structure. The authors can draw the conclusion that the presented ndiNLM algorithm may be useful for some clinical applications such as in perfusion imaging, radiotherapy, tumor surveillance, etc.

Ma, Jianhua; Huang, Jing; Feng, Qianjin; Zhang, Hua; Lu, Hongbing; Liang, Zhengrong; Chen, Wufan [Department of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong 510515, China and Department of Radiology, State University of New York, Stony Brook, New York 11794 (United States); Department of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong 510515 (China); Department of Biomedical Engineering, Fourth Military Medical University, Xi'An, Shanxi 710032 (China); Department of Radiology, State University of New York, Stony Brook, New York 11794 (United States); Department of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong 510515 (China)

2011-10-15T23:59:59.000Z

429

Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans  

SciTech Connect

Microcephaly is a malformation associated with in utero exposed atomic bomb survivors and can be induced in mice by fetal exposure to ionizing radiation (IR). The pathogenesis of IR-induced microcephaly, however, has not been fully understood. Our analyses of high-coverage expression profiling (HiCEP) demonstrated that the abnormal spindle-like microcephaly associated gene (ASPM) was down-regulated in irradiated human diploid fibroblasts. ASPM was recently reported as the causative gene for MCPH-5, the most common type of congenital microcephaly in humans. Here, we show that the expression of the Aspm gene was significantly reduced by IR in various human and murine cells. Additionally, Aspm was found downregulated in the irradiated fetal mouse brain, particularly in the ventricular zones. A similar suppression was observed in the irradiated neurosphere cultures. This is the first report suggesting that the suppression of Aspm by IR could be the initial molecular target leading to the future microcephaly formation.

Fujimori, Akira [Cellular and Molecular Biology Team, National Institute of Radiological Sciences, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan)], E-mail: fujimora@nirs.go.jp; Yaoi, Takeshi; Ogi, Hiroshi [Department of Pathology and Applied Neurobiology, Kyoto Prefectural University of Medicine, Graduate School of Medical Sciences, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan); Wang Bing [National Institute of Radiological Sciences, Heavy-Ion Radiology Research Group, Research Center for Charged Particle Therapy, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Suetomi, Katsutoshi; Sekine, Emiko; Yu Dong; Kato, Takamitsu [Cellular and Molecular Biology Team, National Institute of Radiological Sciences, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Takahashi, Sentaro [National Institute of Radiological Sciences, Heavy-Ion Radiology Research Group, Research Center for Charged Particle Therapy, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Okayasu, Ryuichi [Cellular and Molecular Biology Team, National Institute of Radiological Sciences, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Itoh, Kyoko; Fushiki, Shinji [Department of Pathology and Applied Neurobiology, Kyoto Prefectural University of Medicine, Graduate School of Medical Sciences, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566 (Japan)

2008-05-09T23:59:59.000Z

430

Roles of TNFα Signaling and NFκB in Thiol- and Low Dose  

NLE Websites -- All DOE Office Websites (Extended Search)

Roles of TNFα Signaling and NFκB in Thiol- and Low Dose Radiation-Induced Roles of TNFα Signaling and NFκB in Thiol- and Low Dose Radiation-Induced Adaptive Responses Jeffrey S. Murley The University of Chicago Abstract To better investigate the roles of tumor necrosis factor alpha (TNFα) signaling processes and nuclear transcription factor κB (NFκB) activation on the induction of manganese superoxide dismutase (SOD2) mediated adaptive responses, we employed a two by two experimental matrix that includes the use of both wild type C57BL/6 and C57BL/6 tumor necrosis factor receptor 1 and 2 (TNFR1-R2-) knockout mice, and ras/c-myc transfected wild type and TNFR1-R2- knockout mouse embryo fibroblasts (MEF). MEF were immortalized in order to facilitate their use in our mouse models to test the role of normal or TNFR1-R2- stromal cells and tissues on their responses to thiol

431

TWO-DIMENSIONAL RADIATIVE MAGNETOHYDRODYNAMIC SIMULATIONS OF THE IMPORTANCE OF PARTIAL IONIZATION IN THE CHROMOSPHERE  

Science Conference Proceedings (OSTI)

The bulk of the solar chromosphere is weakly ionized and interactions between ionized particles and neutral particles likely have significant consequences for the thermodynamics of the chromospheric plasma. We investigate the importance of introducing neutral particles into the MHD equations using numerical 2.5D radiative MHD simulations obtained with the Bifrost code. The models span the solar atmosphere from the upper layers of the convection zone to the low corona, and solve the full MHD equations with non-gray and non-LTE radiative transfer, and thermal conduction along the magnetic field. The effects of partial ionization are implemented using the generalized Ohm's law, i.e., we consider the effects of the Hall term and ambipolar diffusion in the induction equation. The approximations required in going from three fluids to the generalized Ohm's law are tested in our simulations. The Ohmic diffusion, Hall term, and ambipolar diffusion show strong variations in the chromosphere. These strong variations of the various magnetic diffusivities are absent or significantly underestimated when, as has been common for these types of studies, using the semi-empirical VAL-C model as a basis for estimates. In addition, we find that differences in estimating the magnitude of ambipolar diffusion arise depending on which method is used to calculate the ion-neutral collision frequency. These differences cause uncertainties in the different magnetic diffusivity terms. In the chromosphere, we find that the ambipolar diffusion is of the same order of magnitude or even larger than the numerical diffusion used to stabilize our code. As a consequence, ambipolar diffusion produces a strong impact on the modeled atmosphere. Perhaps more importantly, it suggests that at least in the chromospheric domain, self-consistent simulations of the solar atmosphere driven by magnetoconvection can accurately describe the impact of the dominant form of resistivity, i.e., ambipolar diffusion. This suggests that such simulations may be more realistic in their approach to the lower solar atmosphere (which directly drives the coronal volume) than previously assumed.

Martinez-Sykora, Juan; De Pontieu, Bart; Hansteen, Viggo, E-mail: j.m.sykora@astro.uio.no [Lockheed Martin Solar and Astrophysics Laboratory, Palo Alto, CA 94304 (United States)

2012-07-10T23:59:59.000Z

432

Low doses of neutrons induce changes in gene expression  

SciTech Connect

Studies were designed to identify genes induced following low-dose neutron but not following {gamma}-ray exposure in fibroblasts. Our past work had shown differences in the expression of {beta}-protein kinase C and c-fos genes, both being induced following {gamma}-ray but not neutron exposure. We have identified two genes that are induced following neutron, but not {gamma}-ray, exposure: Rp-8 (a gene induced by apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency (HIV). Rp-8 mRNA induction was demonstrated in Syrian hamster embryo fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.5 cGy/min) and at high dose rate (12 cGy/min). The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measures of CAT activity and CAT transcripts following irradiation demonstrated an unresponsiveness to {gamma} rays over a broad range of doses. Twofold induction of the HIV-LTR was detected following neutron exposure (48 cGy) administered at low (0.5 cGy/min) but not high (12 cGy/min) dose rates. Ultraviolet-mediated HIV-LTR induction was inhibited by low-dose-rate neutron exposure.

Woloschak, G.E.; Chang-Liu, C.M. [Argonne National Lab., IL (United States); Panozzo, J.; Libertin, C.R. [Loyola Univ., Maywood, IL (United States)

1993-06-01T23:59:59.000Z

433

Low doses of neutrons induce changes in gene expression  

SciTech Connect

Studies were designed to identify genes induced following low-dose neutron but not following [gamma]-ray exposure in fibroblasts. Our past work had shown differences in the expression of [beta]-protein kinase C and c-fos genes, both being induced following [gamma]-ray but not neutron exposure. We have identified two genes that are induced following neutron, but not [gamma]-ray, exposure: Rp-8 (a gene induced by apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency (HIV). Rp-8 mRNA induction was demonstrated in Syrian hamster embryo fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.5 cGy/min) and at high dose rate (12 cGy/min). The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measures of CAT activity and CAT transcripts following irradiation demonstrated an unresponsiveness to [gamma] rays over a broad range of doses. Twofold induction of the HIV-LTR was detected following neutron exposure (48 cGy) administered at low (0.5 cGy/min) but not high (12 cGy/min) dose rates. Ultraviolet-mediated HIV-LTR induction was inhibited by low-dose-rate neutron exposure.

Woloschak, G.E.; Chang-Liu, C.M. (Argonne National Lab., IL (United States)); Panozzo, J.; Libertin, C.R. (Loyola Univ., Maywood, IL (United States))

1993-01-01T23:59:59.000Z

434

Ionization of Sodium and Rubidium nS, nP and nD Rydberg atoms by blackbody radiation  

E-Print Network (OSTI)

Results of theoretical calculations of ionization rates of Rb and Na Rydberg atoms by blackbody radiation (BBR) are presented. Calculations have been performed for nS, nP and nD states of Na and Rb, which are commonly used in a variety of experiments, at principal quantum numbers n=8-65 and at three ambient temperatures of 77, 300 and 600 K. A peculiarity of our calculations is that we take into account the contributions of BBR-induced redistribution of population between Rydberg states prior to photoionization and field ionization by extraction electric field pulses. The obtained results show that these phenomena affect both the magnitude of measured ionization rates and shapes of their dependencies on n. The calculated ionization rates are compared with the results of our earlier measurements of BBR-induced ionization rates of Na nS and nD Rydberg states with n=8-20 at 300 K. A good agreement for all states except nS with n>15 is observed. We also present the useful analytical formulae for quick estimation of BBR ionization rates of Rydberg atoms.

I. I. Beterov; D. B. Tretyakov; I. I. Ryabtsev; A. Ekers; N. N. Bezuglov

2007-02-22T23:59:59.000Z

435

Gaseous Radiochemical Method for Registration of Ionizing Radiation and Its Possible Applications in Science and Industry  

E-Print Network (OSTI)

This work presents a new possibility of registration of ionizing radiation by the flowing gaseous radiochemical method (FGRM). The specified method uses the property of some solid crystalline lattice materials for a free emission of radioactive isotopes of inert gas atoms formed as a result of nuclear reactions. Generated in an ampoule of the detector, the radioactive inert gases are transported by a gas-carrier into the proportional gas counter of the flowing type, where the decay rate of the radioactive gas species is measured. This quantity is unequivocally related to the flux of particles (neutrons, protons, light and heavy ions) at the location of the ampoule. The method was used to monitor the neutron flux of the pulsed neutron target "RADEX" driven by the linear proton accelerator of INR RAS. Further progress of the FGRM may give rise to possible applications in nuclear physics, astrophysics and medicine, in the nondestructive control of fissionable materials, diagnostics of thermonuclear plasma, monitoring of fluxes and measurement of spectra of bombarding particles.

S. G. Lebedev; V. E. Yants

2005-10-06T23:59:59.000Z

436

Ionizing radiation induces IL-6-production by human fibroblasts involving activation of nuclear factor-. kappa. B  

Science Conference Proceedings (OSTI)

The authors report that human lung fibroblasts respond to X-ray treatment with release of interleukin (IL) -6. Synthesis of IL-6 upon ionizing radiation is preceded by an increase of IL-6 transcript levels resulting from transcriptional activation of the IL-6 gene. Analysis of deleted fragments of the IL-6 promoter revealed that transcriptional induction of the IL-6 promoter is due to enhanced binding activity of the transcription factor NF-kB. Although AP-1 does not participate in the rapid induction of the IL-6 promoter its binding activity is also enhanced upon XRT. In contrast to binding kinetics observed with NF-kB, AP-1 binding upon XRT. In contrast to binding kinetics observed with NF-kB- and the AP-1 recognition sequence, conferred inducibility by XRT to a heterologous promoter, with reporter gene activity being maximal 24 hours or 48 hours upon XRT, respectively. Sequential activation of two distinct transcription factors might thus contribute to synchronize transcriptional activation of different genes participating in the X-ray response.

Brach, M.A.; Gruss, H.J.; Kaisho, Tsuneyasu; Asano, Yoshinobu; Vos, Sven de; Mertelsmann, R.; Hirano, Toshio; Herrmann, F. (Univ. of Freiburg (Germany) Osaka Univ. (Japan))

1992-02-26T23:59:59.000Z

437

Ionization of Sodium and Rubidium nS, nP and nD Rydberg atoms by blackbody radiation  

E-Print Network (OSTI)

Results of theoretical calculations of ionization rates of Rb and Na Rydberg atoms by blackbody radiation (BBR) are presented. Calculations have been performed for nS, nP and nD states of Na and Rb, which are commonly used in a variety of experiments, at principal quantum numbers n=8-65 and at three ambient temperatures of 77, 300 and 600 K. A peculiarity of our calculations is that we take into account the contributions of BBR-induced redistribution of population between Rydberg states prior to photoionization and field ionization by extraction electric field pulses. The obtained results show that these phenomena affect both the magnitude of measured ionization rates and shapes of their dependencies on n. The calculated ionization rates are compared with the results of our earlier measurements of BBR-induced ionization rates of Na nS and nD Rydberg states with n=8-20 at 300 K. A good agreement for all states except nS with n>15 is observed. We also present the useful analytical formulae for quick estimation ...

Beterov, I I; Ekers, A; Ryabtsev, I I; Tretyakov, D B

2007-01-01T23:59:59.000Z

438

KUB5/HERA: a dual acting protein that suppresses genomic instability and promotes DNA repair after low dose IR exposure  

NLE Websites -- All DOE Office Websites (Extended Search)

KUB5/HERA: a dual acting protein that suppresses genomic instability and KUB5/HERA: a dual acting protein that suppresses genomic instability and promotes DNA repair after low dose IR exposure Julio C. Morales 1 , Amy Rommel 1 , Konstantin Leskov 2 , Walter M. Hittelman 3 , David A. Boothman 1# 1 Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. 2 Department of Radiation Oncology, Case Western Reserve University, Cleveland, OH 44106, USA. 3 Department of Experimental Therapeutics, M.D. Anderson Cancer Center, Houston, TX 77030, USA. # To whom correspondence should be addressed. E-mail: David.Boothman@utsouthwestern.edu Eukaryotic cells can respond to DNA double strand breaks created by low doses of IR by activating homologous recombination (HR) or non-homologous end- joining (NHEJ) pathways to repair DNA. A yeast two-hybrid screen using Ku70 as

439

European Integrated Project RISC-RAD Radiosensitivity of Individuals and Susceptibility to Cancer induced by Ionizing Radiations  

NLE Websites -- All DOE Office Websites (Extended Search)

Integrated Project RISC-RAD Integrated Project RISC-RAD Radiosensitivity of Individuals and Susceptibility to Cancer induced by Ionizing Radiations Laure Sabatier 1 , L.H.F Mullenders 2 , Mike Atkinson 3 , Simon Bouffler 4 , Herwig Paretzke 5 1 Laboratory of Radiobiology and Oncology, CEA, 18 route du panorama BP6 92265 Fontenay-aux- Roses, France 2 LUMC, Department of Toxicogenetics, Postal Zone S-4-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands 3 GSF- Institute of Pathology, Ingolstädter Landstrasse 1, 85764 Neuherberg Germany 4 HPA Radiation Protection Division, Centre for Radiation Chemical and Environmental Hazards, Chilton, UK 5 GSF- Institute of Radiation Protection, Ingolstädter Landstrasse 1, Neuherberg, D-85764 Germany In radiological protection, the risks of inducing stochastic health effects (largely cancer) by a

440

POST-TRAUMATIC DEGENERATION AND REGENERATION OF SYNAPSES IN THE ACTION OF IONIZING RADIATION  

SciTech Connect

A study was made of degeneration and regeneration of synapses in the upper cervical ganglion and section of the sympathetic trunk on the neck of cats after total single x ray irradiation in a dose of 30, 100, or 500 r. There was an acceleration of these processes after the action of low doses, and delay after the action of high doses. The synapses first restored undergo considerable structural changes with the lapse of time and become small and delicate. The scar at the site of nerve section considerably diminishes in size after the high doses of x-ray irradiation. (auth)

Babmindra, V.P.

1962-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "low-dose ionizing radiation" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Normal Tissue Injury Responses in Mammary Glands After Low Doses...  

NLE Websites -- All DOE Office Websites (Extended Search)

we take advantage of the variation in sensitivity to radiation induced mammary gland cancer in three genetically defined inbred strains of mice (BALBc: sensitive; C57BL6...

442

Mechanisms Underlying Cellular Responses to Low Dose/Low LET...  

NLE Websites -- All DOE Office Websites (Extended Search)

expression, chromosomal and apoptotic analysis suggest a model for strain specific radiation response; normal DNA damage recognition and repair leading to cell death and...

443

Ionizing Radiation Activates AMP-Activated Kinase (AMPK): A Target for Radiosensitization of Human Cancer Cells  

SciTech Connect

Purpose: Adenosine monophosphate (AMP)-activated kinase (AMPK) is a molecular energy sensor regulated by the tumor suppressor LKB1. Starvation and growth factors activate AMPK through the DNA damage sensor ataxia-telangiectasia mutated (ATM). We explored the regulation of AMPK by ionizing radiation (IR) and its role as a target for radiosensitization of human cancer cells. Methods and Materials: Lung, prostate, and breast cancer cells were treated with IR (2-8 Gy) after incubation with either ATM or AMPK inhibitors or the AMPK activator metformin. Then, cells were subjected to either lysis and immunoblotting, immunofluorescence microscopy, clonogenic survival assays, or cell cycle analysis. Results: IR induced a robust phosphorylation and activation of AMPK in all tumor cells, independent of LKB1. IR activated AMPK first in the nucleus, and this extended later into cytoplasm. The ATM inhibitor KU-55933 blocked IR activation of AMPK. AMPK inhibition with Compound C or anti-AMPK {alpha} subunit small interfering RNA (siRNA) blocked IR induction of the cell cycle regulators p53 and p21{sup waf/cip} as well as the IR-induced G2/M arrest. Compound C caused resistance to IR, increasing the surviving fraction after 2 Gy, but the anti-diabetic drug metformin enhanced IR activation of AMPK and lowered the surviving fraction after 2 Gy further. Conclusions: We provide evidence that IR activates AMPK in human cancer cells in an LKB1-independent manner, leading to induction of p21{sup waf/cip} and regulation of the cell cycle and survival. AMPK appears to (1) participate in an ATM-AMPK-p21{sup waf/cip} pathway, (2) be involved in regulation of the IR-induced G2/M checkpoint, and (3) may be targeted by metformin to enhance IR responses.

Sanli, Toran; Rashid, Ayesha; Liu Caiqiong [Department of Oncology, Juravinski Cancer Center and McMaster University, Hamilton, Ontario (Canada)

2010-09-01T23:59:59.000Z

444

3D tissue models for the study of the effects of low-dose irradiation  

NLE Websites -- All DOE Office Websites (Extended Search)

number of more quantitative models of important developmental processes in the mammary gland which can be applied to the study of the interactions between low-dose irradiation,...

445

Long-term Effects of Low-dose Photons on Treg and Other CD4+...  

NLE Websites -- All DOE Office Websites (Extended Search)

and Medical Center Abstract Purpose: The increasing prevalence of low-dose irradiation under work-related and other conditions, e.g. space missions, diagnostic...

446

Soluble/Shed Factors Released from Skin Cells Following Low Dose...  

NLE Websites -- All DOE Office Websites (Extended Search)

SolubleShed Factors Released from Skin Cells Following Low Dose Irradiation Exposure David Springer Pacific Northwest National Laboratory Abstract The purpose of this work is to...

447

Raman spectroscopy of tumour cells exposed to clinically relevant doses of ionizing radiation.  

E-Print Network (OSTI)

??Improvements to radiation therapy treatment outcomes rely, in part, on consideration of patient specific radiosensitivity. Therefore an assay which quantifies radiation-induced biochemical changes, and subsequently (more)

Harder, Samantha

2013-01-01T23:59:59.000Z

448

No evidence for in vivo induction of genomic instability in bone marrow cells collected from mice exposed to low-dose 137Cs γ rays:  

NLE Websites -- All DOE Office Websites (Extended Search)

Rithidech et al, 2006 1 Rithidech et al, 2006 1 No evidence for in vivo induction of genomic instability in bone marrow cells collected from mice exposed to low-dose 137 Cs γ rays: Kanokporn Noy Rithidech 1 , Chatchanok Loetchutinat 1 , Louise Honikel 1 , and Elbert B. Whorton 2 1 Pathology Department, Stony Brook University, Stony Brook, NY 11794-8691 2 Molecular Epidemiology Research Program, University of Texas Medical Branch at Galveston, TX 77555-1153 Assessment of potential health risks associated with exposure to low-dose radiation (at doses below or equal to 0.1 Gy) is still a challenging public health issue. It is therefore important to improve our understanding of potential induction of genomic instability in vivo by this low-dose range because it has been widely suggested that elevation of genomic instability also elevates cancer

449

IONIZATION CHAMBER  

DOE Patents (OSTI)

This patent describes a novel ionization chamber which is well suited to measuring the radioactivity of the various portions of a wire as the wire is moved at a uniform speed, in order to produce the neutron flux traverse pattern of a reactor in which the wire was previously exposed to neutron radiation. The ionization chamber of the present invention is characterized by the construction wherein the wire is passed through a tubular, straight electrode and radiation shielding material is disposed along the wire except at an intermediate, narrow area where the second electrode of the chamber is located.

Redman, W.C.; Shonka, F.R.

1958-02-18T23:59:59.000Z

450

Lung nodule detection in low-dose and thin-slice computed tomography  

Science Conference Proceedings (OSTI)

A computer-aided detection (CAD) system for the identification of small pulmonary nodules in low-dose and thin-slice CT scans has been developed. The automated procedure for selecting the nodule candidates is mainly based on a filter enhancing spherical-shaped ... Keywords: Computer-aided detection (CAD), Image processing, Low-dose computed tomography (LDCT), Thin-slice CT

A. Retico; P. Delogu; M. E. Fantacci; I. Gori; A. Preite Martinez

2008-04-01T23:59:59.000Z

451

Induction of Genomic Instability In Vivo by Low Doses of 137Cs gamma rays  

SciTech Connect

The overall goal of this project is to determine if low doses (below or equal to the level traditionally requiring human radiation protection, i.e. less than or equal to 10 cGy) of low LET radiation can induce genomic instability. The magnitude of genomic instability was measured as delayed chromosome instability in bone marrow cells of exposed mice with different levels of endogenous DNA-dependent protein kinase catalytic subunit (DNA-PKcs) activity, i.e. high (C57BL/6J mice), intermediate (BALB/cJ mice), and extremely low (Scid mice). In addition, at early time points (1 and 4 hrs) following irradiation, levels of activation of nuclear factor-kappa B (NF-{kappa}B), a transcription factor known to be involved in regulating the expression of genes responsible for cell protection following stimuli, were measured in these cells. Bone marrow cells were collected at different times following irradiation, i.e. 1 hr, 4 hrs, 1 month, and 6 months. A total of five mice per dose per strain were sacrificed at each time point for sample collection. As a result, a total of 80 mice from each strain were used. The frequency and the type of metaphase chromosome aberrations in bone marrow cells collected from exposed mice at different times following irradiation were used as markers for radiation-induced genomic instability. A three-color fluorescence in situ hybridization (FISH) protocol for mouse chromosomes 1, 2, and 3 was used for the analysis of delayed stable chromosomal aberrations in metaphase cells. All other visible chromatid-type aberrations and gross structural abnormalities involving non-painted chromosomes were also evaluated on the same metaphase cells used for scoring the stable chromosomal aberrations of painted chromosomes. Levels of nuclear factor-kappa B (NF-{kappa}B) activation were also determined in cells at 1 and 4 hrs following irradiation (indicative of early responses).

Rithidech, Kanokporn; Simon, Sanford, R.; Whorton, Elbert, B.

2006-01-06T23:59:59.000Z

452

Genetic, cell-type, and tissue variation in low-dose and adaptive...  

NLE Websites -- All DOE Office Websites (Extended Search)

goals are to understand the low-dose and adaptive response mechanisms of the mammary gland in order to build a mechanistic model for predicting an individuals risk for...

453

Genetic, cell-type, and tissue variation in low-dose and adaptive...  

NLE Websites -- All DOE Office Websites (Extended Search)

goals are to understand the low-dose and adaptive response mechanisms of the mammary gland in order to build a mechanistic model for predicting an individual's risk for breast...

454

Spectral energy distribution of the metagalactic ionizing radiation field from QSO absorption spectra  

E-Print Network (OSTI)

A computational procedure is presented to estimate the spectral shape of the ionizing background between 1 and 10 Ryd by analyzing optically thin absorption systems in the spectra of high redshift quasars. The procedure is based on the response surface methodology from the theory of experimental design. The shape of the recovered UV background at z~3 shows a significant intensity decrease between 3 and 4 Ryd compared to the metagalactic spectrum of Haardt & Madau (1996). This decrease is interpreted as produced by HeII Gunn-Peterson effect. There are no features indicating a contribution from galaxies to the UV background which is therefore dominated by QSOs at z~3.

I. I. Agafonova; M. Centurion; S. A. Levshakov; P. Molaro

2005-06-07T23:59:59.000Z

455

Ionization chamber  

DOE Patents (OSTI)

An ionization chamber has separate drift and detection regions electrically isolated from each other by a fine wire grid. A relatively weak electric field can be maintained in the drift region when the grid and another electrode in the chamber are connected to a high voltage source. A much stronger electric field can be provided in the detection region by connecting wire electrodes therein to another high voltage source. The detection region can thus be operated in a proportional mode when a suitable gas is contained in the chamber. High resolution output pulse waveforms are provided across a resistor connected to the detection region anode, after ionizing radiation enters the drift region and ionize the gas.

Walenta, Albert H. (Port Jefferson Station, NY)

1981-01-01T23:59:59.000Z

456

ealth physics is concerned with protecting people from the harmful effects of ionizing radiation while allowing its beneficial use in medicine, science,  

E-Print Network (OSTI)

, particularly from medical exposures and from the atomic-bomb ex- posures in Hiroshima and Nagasaki. DuringH ealth physics is concerned with protecting people from the harmful effects of ionizing radiation effects such as cancer that had been observed in populations of people receiv- ing high doses

Massey, Thomas N.

457

The status of low dose rate and future of high dose rate Cf-252 brachytherapy  

Science Conference Proceedings (OSTI)

This work describes the current status of the US low dose rate (LDR) Cf-252 brachytherapy program. The efforts undertaken towards development of a high dose rate (HDR) remotely after loaded Cf-252 source, which can accommodate 1 mg or greater Cf-252, are also described. This HDR effort is a collaboration between Oak Ridge National Laboratory (ORNL), commercial remote after loader manufactures, the Gershenson Radiation Oncology Center (ROC), and Wayne State University. To achieve this goal, several advances in isotope chemistry and source preparation at ORNL must be achieved to yield a specific material source loading of greater than or equal 1 mg Cf-252 per mm3. Development work with both radioactive and non-radioactive stand-ins for Cf-252 have indicated the feasibility of fabricating such sources. As a result, the decreased catheter diameter and computer controlled source placement will permit additional sites (e.g. brain, breast, prostate, lung, parotid, etc.) to be treated effectively with Cf-252 sources. Additional work at the Radiochemical Engineering and Development Center (REDC) remains in source fabrication, after loader modification, and safe design. The current LDR Cf-252 Treatment Suite at the ROC is shielded and licensed to hold up to 1 mg of Cf-252. This was designed to maintain cumulative personnel exposure, both external to the room and in direct isotope handling, at less than 20 microSv/hr. However, cumulative exposure may be greatly decreased if a Cf-252 HDR unit is employed which would eliminate direct isotope handling and decrease treatment times from tilde 3 hours to an expected range of 3 to 15 minutes. Such a Cf-252 HDR source will also demonstrate improved dose distributions over current LDR treatments due to the ability to step the point-like source throughout the target volume and weight the dwell time accordingly.

Rivard, M.J.; Wierzbicki, J.G.; Van den Heuvel, F.; Chuba, P.J.; Fontanesi, J. [Wayne State Univ., Detroit, MI (United States). Dept. of Radiation Oncology; Martin, R.C.; McMahon, R.R.; Haire, R.G. [Oak Ridge National Lab., TN (United States)

1997-12-01T23:59:59.000Z

458

Ionizing radiation risks to Satellite Power Systems (SPS) workers in space  

DOE Green Energy (OSTI)

A reference Satellite Power System (SPS) has been designed by NASA and its contractors for the purposes of evaluating the concept and carrying out assessments of the various consequences of development, including those on the health of the space workers. The Department of Energy has responsibility for directing various assessments. Present planning calls for the SPS workers to move from Earth to a low earth orbit (LEO) at an altitude of 500 kilometers; to travel by a transfer ellipse (TE) trajectory to a geosynchronous orbit (GEO) at an altitude of 36,000 kilometers; and to remain in GEO orbit for about 90 percent of the total time aloft. The radiation risks to the health of workers who will construct and maintain solar power satellites in the space environment are studied. The charge to the committee was: (a) to evaluate the radiation environment estimated for the Reference System which could represent a hazard; (b) to assess the possible somatic and genetic radiation hazards; and (c) to estimate the risks to the health of SPS workers due to space radiation exposure, and to make recommendations based on these conclusions. Details are presented. (WHK)

Not Available

1980-12-01T23:59:59.000Z

459

A molecular dynamics simulation of DNA damage induction by ionizing radiation  

E-Print Network (OSTI)

We present a multi-scale simulation of early stage of DNA damages by the indirect action of hydroxyl ($^\\bullet$OH) free radicals generated by electrons and protons. The computational method comprises of interfacing the Geant4-DNA Monte Carlo with the ReaxFF molecular dynamics software. A clustering method was employed to map the coordinates of $^\\bullet$OH-radicals extracted from the ionization track-structures onto nano-meter simulation voxels filled with DNA and water molecules. The molecular dynamics simulation provides the time evolution and chemical reactions in individual simulation voxels as well as the energy-landscape accounted for the DNA-$^\\bullet$OH chemical reaction that is essential for the first principle enumeration of hydrogen abstractions, chemical bond breaks, and DNA-lesions induced by collection of ions in clusters less than the critical dimension which is approximately 2-3 \\AA. We show that the formation of broken bonds leads to DNA base and backbone damages that collectively propagate ...

Abolfath, Ramin M; Chen, Zhe J; Nath, Ravinder

2013-01-01T23:59:59.000Z

460

Protein Kinase CK2 Regulates Cytoskeletal Reorganization during Ionizing Radiation-Induced Senescence of Human Mesenchymal Stem Cells  

SciTech Connect

Human mesenchymal stem cells (hMSC) are critical for tissue regeneration. How hMSC respond to genotoxic stresses and potentially contribute to aging and cancer remain underexplored. We demonstrated that ionizing radiation induced cellular senescence of hMSC over a period of 10 days, showing a critical transition between day 3 and day 6. This was confirmed by senescence-associated beta-galactosidase (SA-{beta}-gal) staining, protein expression profiles of key cell cycle regulators (retinoblastoma (Rb) protein, p53, p21{sup waf1/Cip1}, and p16{sup INK4A}), and senescence-associated secre