National Library of Energy BETA

Sample records for lignocellulose recalcitrance screening

  1. High-Throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

    SciTech Connect (OSTI)

    Decker, Stephen R.; Sykes, Robert W.; Turner, Geoffrey B.; Lupoi, Jason S.; Doepkke, Crissa; Tucker, Melvin P.; Schuster, Logan A.; Mazza, Kimberly; Himmel, Michael E.; Davis, Mark F.; Gjersing, Erica

    2015-09-15

    The conversion of lignocellulosic biomass to fuels, chemicals, and other commodities has been explored as one possible pathway toward reductions in the use of non-renewable energy sources. In order to identify which plants, out of a diverse pool, have the desired chemical traits for downstream applications, attributes, such as cellulose and lignin content, or monomeric sugar release following an enzymatic saccharification, must be compared. The experimental and data analysis protocols of the standard methods of analysis can be time-consuming, thereby limiting the number of samples that can be measured. High-throughput (HTP) methods alleviate the shortcomings of the standard methods, and permit the rapid screening of available samples to isolate those possessing the desired traits. This study illustrates the HTP sugar release and pyrolysis-molecular beam mass spectrometry pipelines employed at the National Renewable Energy Lab. These pipelines have enabled the efficient assessment of thousands of plants while decreasing experimental time and costs through reductions in labor and consumables.

  2. Advances in High Throughput Screening of Biomass Recalcitrance (Poster)

    SciTech Connect (OSTI)

    Turner, G. B.; Decker, S. R.; Tucker, M. P.; Law, C.; Doeppke, C.; Sykes, R. W.; Davis, M. F.; Ziebell, A.

    2012-06-01

    This was a poster displayed at the Symposium. Advances on previous high throughput screening of biomass recalcitrance methods have resulted in improved conversion and replicate precision. Changes in plate reactor metallurgy, improved preparation of control biomass, species-specific pretreatment conditions, and enzymatic hydrolysis parameters have reduced overall coefficients of variation to an average of 6% for sample replicates. These method changes have improved plate-to-plate variation of control biomass recalcitrance and improved confidence in sugar release differences between samples. With smaller errors plant researchers can have a higher degree of assurance more low recalcitrance candidates can be identified. Significant changes in plate reactor, control biomass preparation, pretreatment conditions and enzyme have significantly reduced sample and control replicate variability. Reactor plate metallurgy significantly impacts sugar release aluminum leaching into reaction during pretreatment degrades sugars and inhibits enzyme activity. Removal of starch and extractives significantly decreases control biomass variability. New enzyme formulations give more consistent and higher conversion levels, however required re-optimization for switchgrass. Pretreatment time and temperature (severity) should be adjusted to specific biomass types i.e. woody vs. herbaceous. Desalting of enzyme preps to remove low molecular weight stabilizers and improved conversion levels likely due to water activity impacts on enzyme structure and substrate interactions not attempted here due to need to continually desalt and validate precise enzyme concentration and activity.

  3. High Throughput Pretreatment and Enzyme Hydrolysis of Biomass: Screening Recalcitrance in Large Sample Populations (Presentation)

    SciTech Connect (OSTI)

    Decker, S. R.

    2010-10-01

    Presentation on the execution of the first high-throughput thermochemical pretreatment/enzyme digestion pipeline for screening biomass for recalcitrance.

  4. Evaluation of High Throughput Screening Methods in Picking up Differences between Cultivars of Lignocellulosic Biomass for Ethanol Production

    SciTech Connect (OSTI)

    Lindedam, Jane; Bruun, Sander; Jorgensen, Henning; Decker, Stephen R.; Turner, Geoffrey B.; DeMartini, Jaclyn D.; Wyman, Charles E.; Felby, Claus

    2014-07-01

    Here, we present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. We found the best correlation of glucose yields between the Riverside and NREL systems (R2 = 0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2 = 0.4269). The three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.

  5. High-Solids Enzymatic Saccharification Screening Method for Lignocellulosic Biomass (Poster)

    SciTech Connect (OSTI)

    Roche, C. M.; Stickel, J. J.

    2009-05-01

    The ability to screen new biomass pretreatments and advanced enzyme systems at process-relevant conditions is key to developing economically viable lignocellulosic ethanol. While much research is being invested in developing pretreatment technologies and enzyme systems that will more efficiently convert cellulosic biomass to sugars, the current standard reactor vessel, a shake flask, that is used for screening enzymatic saccharification of cellulosic biomass is inadequate at high-solids conditions. Shake flasks do not provide adequate mixing at high solids conditions. In this work, a roller bottle reactor was identified as a small-scale high-solids saccharification reaction vessel, and a method was developed for use in screening both pretreated biomass and enzyme systems at process-relevant conditions. This new method addresses mixing issues observed in high-solids saccharifications. In addition, yield calculations from sugar concentrations on a mass basis were used to account for the two-phase nature of the saccharification slurry, which eliminates discontinuities in comparing high-solids to low-solids saccharifications that occur when using concentrations on a volume basis. The roller bottle reactors out-performed the shake flasks by 5% for an initial insoluble solids loading of 15% and 140% for an initial soluble solids loading of 30%. The reactor system and method was compared at bench and floor scales and determined to be scalable for initial insoluble solids loading in the range of 15% to 30%. Pretreatment and enzyme screening results indicate that mid severity pretreated biomass is more digestible than the low and high severity biomass and GC220 is a superior enzyme to Spezyme CP.

  6. Novel System for Recalcitrance Screening Will Reduce Biofuels Production Costs; The Spectrum of Clean Energy Innovation (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2010-06-01

    Fact sheet describes a high-throughput screening process, developed at NREL, that enables researchers to screen a large variety of biomass feedstocks for traits that indicate they would easily convert to fermentable sugars.

  7. Center for Lignocellulose Structure and Function - Our Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Research The Center for Lignocellulose Structure and Formation (CLSF) is focused on developing a detailed understanding of lignocellulose, the main structural material in plants. Perspective Every living organism on Earth uses glucose as an energy source. Plants not only make glucose from sunlight, water and CO2, but they convert much of it into an energy-rich material - the lignocellulosic cell wall - that is both a versatile material and a recalcitrant feedstock for liquid biofuel production,

  8. Lignocellulosic Biomass

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lignocellulosic Biomass - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced

  9. Ethanol production from lignocellulose

    DOE Patents [OSTI]

    Ingram, Lonnie O.; Wood, Brent E.

    2001-01-01

    This invention presents a method of improving enzymatic degradation of lignocellulose, as in the production of ethanol from lignocellulosic material, through the use of ultrasonic treatment. The invention shows that ultrasonic treatment reduces cellulase requirements by 1/3 to 1/2. With the cost of enzymes being a major problem in the cost-effective production of ethanol from lignocellulosic material, this invention presents a significant improvement over presently available methods.

  10. Prehydrolysis of lignocellulose

    DOE Patents [OSTI]

    Torget, Robert W.; Kadam, Kiran L.; Hsu, Teh-An; Philippidis, George P.; Wyman, Charles E.

    1995-01-01

    The invention relates to the prehydrolysis of lignocellulose by passing an acidic or alkaline solution through solid lignocellulosic particles with removal of soluble components as they are formed. The technique permits a less severe combination of pH, temperature and time than conventional prehydrolysis. Furthermore, greater extraction of both hemicellulose and lignin occurs simultaneously in the same reactor and under the same conditions.

  11. Prehydrolysis of lignocellulose

    DOE Patents [OSTI]

    Torget, Robert W.; Kadam, Kiran L.; Hsu, Teh-An; Philippidis, George P.; Wyman, Charles E.

    1996-01-01

    The invention relates to the prehydrolysis of lignocellulose by passing an acidic or alkaline solution through solid lignocellulosic particles with removal of soluble components as they are formed. The technique permits a less severe combination of pH, temperature and time than conventional prehydrolysis. Furthermore, greater extraction of both hemicellulose and lignin occurs simultaneously in the same reactor and under the same conditions.

  12. Prehydrolysis of lignocellulose

    DOE Patents [OSTI]

    Torget, Robert W.; Kadam, Kiran L.; Hsu, Teh-An; Philippidis, George P.; Wyman, Charles E.

    1998-01-01

    The invention relates to the prehydrolysis of lignocellulose by passing an acidic or alkaline solution through solid lignocellulosic particles with removal of soluble components as they are formed. The technique permits a less severe combination of pH, temperature and time than conventional prehydrolysis. Furthermore, greater extraction of both hemicellulose and lignin occurs simultaneously in the same reactor and under the same conditions.

  13. Prehydrolysis of lignocellulose

    DOE Patents [OSTI]

    Torget, R.W.; Kadam, K.L.; Hsu, T.A.; Philippidis, G.P.; Wyman, C.E.

    1996-04-02

    The invention relates to the prehydrolysis of lignocellulose by passing an acidic or alkaline solution through solid lignocellulosic particles with removal of soluble components as they are formed. The technique permits a less severe combination of pH, temperature and time than conventional prehydrolysis. Furthermore, greater extraction of both hemicellulose and lignin occurs simultaneously in the same reactor and under the same conditions. 7 figs.

  14. Prehydrolysis of lignocellulose

    DOE Patents [OSTI]

    Torget, R.W.; Kadam, K.L.; Hsu, T.A.; Philippidis, G.P.; Wyman, C.E.

    1995-06-13

    The invention relates to the prehydrolysis of lignocellulose by passing an acidic or alkaline solution through solid lignocellulosic particles with removal of soluble components as they are formed. The technique permits a less severe combination of pH, temperature and time than conventional prehydrolysis. Furthermore, greater extraction of both hemicellulose and lignin occurs simultaneously in the same reactor and under the same conditions. 7 figs.

  15. Prehydrolysis of lignocellulose

    DOE Patents [OSTI]

    Torget, R.W.; Kadam, K.L.; Hsu, T.A.; Philippidis, G.P.; Wyman, C.E.

    1998-01-06

    The invention relates to the prehydrolysis of lignocellulose by passing an acidic or alkaline solution through solid lignocellulosic particles with removal of soluble components as they are formed. The technique permits a less severe combination of pH, temperature and time than conventional prehydrolysis. Furthermore, greater extraction of both hemicellulose and lignin occurs simultaneously in the same reactor and under the same conditions. 7 figs.

  16. Method for pretreating lignocellulosic biomass

    DOE Patents [OSTI]

    Kuzhiyil, Najeeb M.; Brown, Robert C.; Dalluge, Dustin Lee

    2015-08-18

    The present invention relates to a method for pretreating lignocellulosic biomass containing alkali and/or alkaline earth metal (AAEM). The method comprises providing a lignocellulosic biomass containing AAEM; determining the amount of the AAEM present in the lignocellulosic biomass; identifying, based on said determining, the amount of a mineral acid sufficient to completely convert the AAEM in the lignocellulosic biomass to thermally-stable, catalytically-inert salts; and treating the lignocellulosic biomass with the identified amount of the mineral acid, wherein the treated lignocellulosic biomass contains thermally-stable, catalytically inert AAEM salts.

  17. Evaluating lignocellulosic biomass, its derivatives, and downstream products with Raman spectroscopy

    SciTech Connect (OSTI)

    Lupoi, Jason S.; Gjersing, Erica; Davis, Mark F.

    2015-04-20

    The creation of fuels, chemicals, and materials from plants can aid in replacing products fabricated from non-renewable energy sources. Before using biomass in downstream applications, it must be characterized to assess chemical traits, such as cellulose, lignin, or lignin monomer content, or the sugars released following an acid or enzymatic hydrolysis. The measurement of these traits allows researchers to gage the recalcitrance of the plants and develop efficient deconstruction strategies to maximize yields. Standard methods for assessing biomass phenotypes often have experimental protocols that limit their use for screening sizeable numbers of plant species. Raman spectroscopy, a non-destructive, non-invasive vibrational spectroscopy technique, is capable of providing qualitative, structural information and quantitative measurements. Applications of Raman spectroscopy have aided in alleviating the constraints of standard methods by coupling spectral data with multivariate analysis to construct models capable of predicting analytes. Hydrolysis and fermentation products, such as glucose and ethanol, can be quantified off-, at-, or on-line. Raman imaging has enabled researchers to develop a visual understanding of reactions, such as different pretreatment strategies, in real-time, while also providing integral chemical information. Finally, this review provides an overview of what Raman spectroscopy is, and how it has been applied to the analysis of whole lignocellulosic biomass, its derivatives, and downstream process monitoring.

  18. Evaluating lignocellulosic biomass, its derivatives, and downstream products with Raman spectroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lupoi, Jason S.; Gjersing, Erica; Davis, Mark F.

    2015-04-20

    The creation of fuels, chemicals, and materials from plants can aid in replacing products fabricated from non-renewable energy sources. Before using biomass in downstream applications, it must be characterized to assess chemical traits, such as cellulose, lignin, or lignin monomer content, or the sugars released following an acid or enzymatic hydrolysis. The measurement of these traits allows researchers to gage the recalcitrance of the plants and develop efficient deconstruction strategies to maximize yields. Standard methods for assessing biomass phenotypes often have experimental protocols that limit their use for screening sizeable numbers of plant species. Raman spectroscopy, a non-destructive, non-invasive vibrationalmore » spectroscopy technique, is capable of providing qualitative, structural information and quantitative measurements. Applications of Raman spectroscopy have aided in alleviating the constraints of standard methods by coupling spectral data with multivariate analysis to construct models capable of predicting analytes. Hydrolysis and fermentation products, such as glucose and ethanol, can be quantified off-, at-, or on-line. Raman imaging has enabled researchers to develop a visual understanding of reactions, such as different pretreatment strategies, in real-time, while also providing integral chemical information. Finally, this review provides an overview of what Raman spectroscopy is, and how it has been applied to the analysis of whole lignocellulosic biomass, its derivatives, and downstream process monitoring.« less

  19. BSCL use plan: Solving biomass recalcitrance

    SciTech Connect (OSTI)

    Himmel, M.; Vinzant, T.; Bower, S.; Jechura, J.

    2005-08-01

    Saccharification of lignocellulosic biomass has long been recognized as a potential low-cost source of mixed sugars for fermentation to fuel ethanol or chemicals. Several technologies have been developed over the years that allow this conversion process to occur, yet the significant challenge remaining is to make the process cost competitive.

  20. Fuels and Chemicals from Lignocellulosic Biomass: Valorization...

    Office of Scientific and Technical Information (OSTI)

    Fuels and Chemicals from Lignocellulosic Biomass: Valorization of Lignin. Citation Details In-Document Search Title: Fuels and Chemicals from Lignocellulosic Biomass: Valorization ...

  1. Methods for degrading lignocellulosic materials

    DOE Patents [OSTI]

    Vlasenko, Elena; Cherry, Joel; Xu, Feng

    2008-04-08

    The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermentating microoganisms; and (c) recovering the organic substance from the fermentation.

  2. Methods for degrading lignocellulosic materials

    DOE Patents [OSTI]

    Vlasenko, Elena; Cherry, Joel; Xu, Feng

    2011-05-17

    The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermenting microorganisms; and (c) recovering the organic substance from the fermentation.

  3. Screening

    Office of Energy Efficiency and Renewable Energy (EERE)

    Screening is typically performed by an outside party or an independent renewable energy expert or team. It is a review of the possible technology options that identifies dead-ends and further...

  4. BETO Webinar: Computational Studies of Lignocellulose Deconstruction |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Webinar: Computational Studies of Lignocellulose Deconstruction BETO Webinar: Computational Studies of Lignocellulose Deconstruction Dr. Gnanakaran of Los Alamos National Laboratory (LANL) presented LANL's molecular research on lignocellulose on April 15, 2013, at the Bioenergy Technologies Office's webinar series. april2013_lanl_webinar.pdf (5.81 MB) More Documents & Publications Process Design and Economics for the Conversion of Lignocellulosic Biomass to

  5. Identification and overexpression of a knotted1-like transcription factor in switchgrass (Panicum virgatum L.) for lignocellulosic feedstock improvement

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wuddineh, Wegi A.; Mazarei, Mitra; Zhang, Ji -Yi; Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.; Udvardi, Michael K.; C. Neal Stewart, Jr.

    2016-04-28

    High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expressionmore » of the endogenous PvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our findings demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics.« less

  6. An Index-Based Approach to Assessing Recalcitrance and Soil Carbon Sequestration Potential of Engineered Black Carbons (Biochars)

    SciTech Connect (OSTI)

    Harvey, Omar R.; Kuo, Li-Jung; Zimmerman, Andrew R.; Louchouarn, Patrick; Amonette, James E.; Herbert, Bruce

    2012-01-10

    The ability of engineered black carbons (or biochars) to resist abiotic and, or biotic degradation (herein referred to as recalcitrance) is crucial to their successful deployment as a soil carbon sequestration strategy. A new recalcitrance index, the R{sub 50}, for assessing biochar quality for carbon sequestration is proposed. The R{sub 50} is based on the relative thermal stability of a given biochar to that of graphite and was developed and evaluated with a variety of biochars (n = 59), and soot-like black carbons. Comparison of R{sub 50}, with biochar physicochemical properties and biochar-C mineralization revealed the existence of a quantifiable relationship between R{sub 50} and biochar recalcitrance. As presented here, the R{sub 50} is immediately applicable to pre-land application screening of biochars into Class A (R{sub 50} {>=} 0.70), Class B (0.50 {<=} R{sub 50} < 0.70) or Class C (R{sub 50} < 0.50) recalcitrance/carbon sequestration classes. Class A and Class C biochars would have carbon sequestration potential comparable to soot/graphite and uncharred plant biomass, respectively, while Class B biochars would have intermediate carbon sequestration potential. We believe that the coupling of the R{sub 50}, to an index-based degradation, and an economic model could provide a suitable framework in which to comprehensively assess soil carbon sequestration in biochars.

  7. Preprocessing Moist Lignocellulosic Biomass for Biorefinery Feedstocks

    SciTech Connect (OSTI)

    Neal Yancey; Christopher T. Wright; Craig Conner; J. Richard Hess

    2009-06-01

    Biomass preprocessing is one of the primary operations in the feedstock assembly system of a lignocellulosic biorefinery. Preprocessing is generally accomplished using industrial grinders to format biomass materials into a suitable biorefinery feedstock for conversion to ethanol and other bioproducts. Many factors affect machine efficiency and the physical characteristics of preprocessed biomass. For example, moisture content of the biomass as received from the point of production has a significant impact on overall system efficiency and can significantly affect the characteristics (particle size distribution, flowability, storability, etc.) of the size-reduced biomass. Many different grinder configurations are available on the market, each with advantages under specific conditions. Ultimately, the capacity and/or efficiency of the grinding process can be enhanced by selecting the grinder configuration that optimizes grinder performance based on moisture content and screen size. This paper discusses the relationships of biomass moisture with respect to preprocessing system performance and product physical characteristics and compares data obtained on corn stover, switchgrass, and wheat straw as model feedstocks during Vermeer HG 200 grinder testing. During the tests, grinder screen configuration and biomass moisture content were varied and tested to provide a better understanding of their relative impact on machine performance and the resulting feedstock physical characteristics and uniformity relative to each crop tested.

  8. Catalytic fast pyrolysis of lignocellulosic biomass (Journal...

    Office of Scientific and Technical Information (OSTI)

    Thus, converting lignocellulose into transportation fuels via catalytic fast pyrolysis has attracted much attention. Many studies related to catalytic fast pyrolysis of biomass ...

  9. Genetic manipulation of lignocellulosic biomass for bioenergy...

    Office of Scientific and Technical Information (OSTI)

    biomass for bioenergy Citation Details In-Document Search This content will become publicly available on September 7, 2017 Title: Genetic manipulation of lignocellulosic biomass ...

  10. Lignocellulosic Biomass to Ethanol Process Design and Economics...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current ... Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current ...

  11. Development of a Commerical Enzyme System for Lignocellulosic Biomass Saccharification

    SciTech Connect (OSTI)

    Manoj Kumar, PhD

    2011-02-14

    Lignocellulosic biomass is the most abundant, least expensive renewable natural biological resource for the production of biobased products and bioenergy is important for the sustainable development of human civilization in 21st century. For making the fermentable sugars from lignocellulosic biomass, a reduction in cellulase production cost, an improvement in cellulase performance, and an increase in sugar yields are all vital to reduce the processing costs of biorefineries. Improvements in specific cellulase activities for non-complexed cellulase mixtures can be implemented through cellulase engineering based on rational design or directed evolution for each cellulase component enzyme, as well as on the reconstitution of cellulase components. In this paper, we will provide DSM's efforts in cellulase research and developments and focus on limitations. Cellulase improvement strategies based on directed evolution using screening on relevant substrates, screening for higher thermal tolerance based on activity screening approaches such as continuous culture using insoluble cellulosic substrates as a powerful selection tool for enriching beneficial cellulase mutants from the large library. We will illustrate why and how thermostable cellulases are vital for economic delivery of bioproducts from cellulosic biomass using biochemical conversion approach.

  12. lignocellulose

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power ...

  13. Ethanol production with dilute acid hydrolysis using partially dried lignocellulosics

    DOE Patents [OSTI]

    Nguyen, Quang A.; Keller, Fred A.; Tucker, Melvin P.

    2003-12-09

    A process of converting lignocellulosic biomass to ethanol, comprising hydrolyzing lignocellulosic materials by subjecting dried lignocellulosic material in a reactor to a catalyst comprised of a dilute solution of a strong acid and a metal salt to lower the activation energy (i.e., the temperature) of cellulose hydrolysis and ultimately obtain higher sugar yields.

  14. Development of a commercial enzymes system for lignocellulosic biomass saccharification

    SciTech Connect (OSTI)

    Kumar, Manoj

    2012-12-20

    DSM Innovation Inc., in its four year effort was able to evaluate and develop its in-house DSM fungal cellulolytic enzymes system to reach enzyme efficiency mandates set by DoE Biomass program MYPP goals. DSM enzyme cocktail is uniquely active at high temperature and acidic pH, offering many benefits and product differentiation in 2G bioethanol production. Under this project, strain and process development, ratio optimization of enzymes, protein and genetic engineering has led to multitudes of improvement in productivity and efficiency making development of a commercial enzyme system for lignocellulosic biomass saccharification viable. DSM is continuing further improvement by additional biodiversity screening, protein engineering and overexpression of enzymes to continue to further lower the cost of enzymes for saccharification of biomass.

  15. Catalytic fast pyrolysis of lignocellulosic biomass

    SciTech Connect (OSTI)

    Liu, Changjun; Wang, Huamin; Karim, Ayman M.; Sun, Junming; Wang, Yong

    2014-11-21

    Increasing energy demand, especially in the transportation sector, and soaring CO2 emissions necessitate the exploitation of renewable sources of energy. Despite the large variety of new energy Q3 carriers, liquid hydrocarbon still appears to be the most attractive and feasible form of transportation fuel taking into account the energy density, stability and existing infrastructure. Biomass is an abundant, renewable source of energy; however, utilizing it in a cost-effective way is still a substantial challenge. Lignocellulose is composed of three major biopolymers, namely cellulose, hemicellulose and lignin. Fast pyrolysis of biomass is recognized as an efficient and feasible process to selectively convert lignocellulose into a liquid fuel—bio-oil. However bio-oil from fast pyrolysis contains a large amount of oxygen, distributed in hundreds of oxygenates. These oxygenates are the cause of many negative properties, such as low heating values, high corrosiveness, high viscosity, and instability; they also greatly Q4 limit the application of bio-oil particularly as transportation fuel. Hydrocarbons derived from biomass are most attractive because of their high energy density and compatibility with the existing infrastructure. Thus, converting lignocellulose into transportation fuels via catalytic fast pyrolysis has attracted much attention. Many studies related to catalytic fast pyrolysis of biomass have been published. The main challenge of this process is the development of active and stable catalysts that can deal with a large variety of decomposition intermediates from lignocellulose. This review starts with the current understanding of the chemistry in fast pyrolysis of lignocellulose and focuses on the development of catalysts in catalytic fast pyrolysis. Recent progress in the experimental studies on catalytic fast pyrolysis of biomass is also summarized with the emphasis on bio-oil yields and quality.

  16. Lignocellulosic Biomass to Ethanol Process Design and Economics...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis For Corn Stover Lignocellulosic Biomass to Ethanol Process Design ...

  17. Process Design and Economics for the Conversion of Lignocellulosic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion ...

  18. Processes for converting lignocellulosics to reduced acid pyrolysis oil

    DOE Patents [OSTI]

    Kocal, Joseph Anthony; Brandvold, Timothy A

    2015-01-06

    Processes for producing reduced acid lignocellulosic-derived pyrolysis oil are provided. In a process, lignocellulosic material is fed to a heating zone. A basic solid catalyst is delivered to the heating zone. The lignocellulosic material is pyrolyzed in the presence of the basic solid catalyst in the heating zone to create pyrolysis gases. The oxygen in the pyrolysis gases is catalytically converted to separable species in the heating zone. The pyrolysis gases are removed from the heating zone and are liquefied to form the reduced acid lignocellulosic-derived pyrolysis oil.

  19. Hydrolysis and fractionation of lignocellulosic biomass

    DOE Patents [OSTI]

    Torget, Robert W.; Padukone, Nandan; Hatzis, Christos; Wyman, Charles E.

    2000-01-01

    A multi-function process is described for the hydrolysis and fractionation of lignocellulosic biomass to separate hemicellulosic sugars from other biomass components such as extractives and proteins; a portion of the solubilized lignin; cellulose; glucose derived from cellulose; and insoluble lignin from said biomass comprising one or more of the following: optionally, as function 1, introducing a dilute acid of pH 1.0-5.0 into a continual shrinking bed reactor containing a lignocellulosic biomass material at a temperature of about 94 to about 160.degree. C. for a period of about 10 to about 120 minutes at a volumetric flow rate of about 1 to about 5 reactor volumes to effect solubilization of extractives, lignin, and protein by keeping the solid to liquid ratio constant throughout the solubilization process; as function 2, introducing a dilute acid of pH 1.0-5.0, either as virgin acid or an acidic stream from another function, into a continual shrinking bed reactor containing either fresh biomass or the partially fractionated lignocellulosic biomass material from function 1 at a temperature of about 94-220.degree. C. for a period of about 10 to about 60 minutes at a volumetric flow rate of about 1 to about 5 reactor volumes to effect solubilization of hemicellulosic sugars, semisoluble sugars and other compounds, and amorphous glucans by keeping the solid to liquid ratio constant throughout the solubilization process; as function 3, optionally, introducing a dilute acid of pH 1.0-5.0 either as virgin acid or an acidic stream from another function, into a continual shrinking bed reactor containing the partially fractionated lignocellulosic biomass material from function 2 at a temperature of about 180-280.degree. C. for a period of about 10 to about 60 minutes at a volumetric flow rate of 1 to about 5 reactor volumes to effect solubilization of cellulosic sugars by keeping the solid to liquid ratio constant throughout the solubilization process; and as function 4

  20. High-Speed Biomass Recalcitrance Pipeline Speeds Up Bio-Mass Analysis -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Innovation Portal High-Speed Biomass Recalcitrance Pipeline Speeds Up Bio-Mass Analysis Robotic pipeline allows for rapid analysis of optimal substrate/enzyme combination for efficient bio-fuel production. National Renewable Energy Laboratory Ames Laboratory Contact NREL About This Technology Technology Marketing SummaryPipeline analysis speeds up the process for the selection of plant species with the lowest natural recalcitrance (resistance to sugar conversion) as well as the

  1. Fully Integrated Lignocellulosic Biorefinery with Onsite Production of Enzymes and Yeast

    SciTech Connect (OSTI)

    Manoj Kumar, PhD

    2010-06-14

    Lignocellulosic biomass is the most abundant, least expensive renewable natural biological resource for the production of biobased products and bioenergy is important for the sustainable development of human civilization in 21st century. For making the fermentable sugars from lignocellulosic biomass, a reduction in cellulase production cost, an improvement in cellulase performance, and an increase in sugar yields are all vital to reduce the processing costs of biorefineries. Improvements in specific cellulase activities for non-complexed cellulase mixtures can be implemented through cellulase engineering based on rational design or directed evolution for each cellulase component enzyme, as well as on the reconstitution of cellulase components. In this paper, we will provide DSM's efforts in cellulase research and developments and focus on limitations. Cellulase improvement strategies based on directed evolution using screening on relevant substrates, screening for higher thermal tolerance based on activity screening approaches such as continuous culture using insoluble cellulosic substrates as a powerful selection tool for enriching beneficial cellulase mutants from the large library. We will illustrate why and how thermostable cellulases are vital for economic delivery of bioproducts from cellulosic biomass using biochemical conversion approach.

  2. Dilute acid/metal salt hydrolysis of lignocellulosics

    DOE Patents [OSTI]

    Nguyen, Quang A.; Tucker, Melvin P.

    2002-01-01

    A modified dilute acid method of hydrolyzing the cellulose and hemicellulose in lignocellulosic material under conditions to obtain higher overall fermentable sugar yields than is obtainable using dilute acid alone, comprising: impregnating a lignocellulosic feedstock with a mixture of an amount of aqueous solution of a dilute acid catalyst and a metal salt catalyst sufficient to provide higher overall fermentable sugar yields than is obtainable when hydrolyzing with dilute acid alone; loading the impregnated lignocellulosic feedstock into a reactor and heating for a sufficient period of time to hydrolyze substantially all of the hemicellulose and greater than 45% of the cellulose to water soluble sugars; and recovering the water soluble sugars.

  3. Tower reactors for bioconversion of lignocellulosic material

    DOE Patents [OSTI]

    Nguyen, Quang A.

    1998-01-01

    An apparatus for enzymatic hydrolysis and fermentation of pretreated lignocellulosic material, in the form of a tower bioreactor, having mixers to achieve intermittent mixing of the material. Precise mixing of the material is important for effective heat and mass transfer requirements without damaging or denaturing the enzymes or fermenting microorganisms. The pretreated material, generally in the form of a slurry, is pumped through the bioreactor, either upwards of downwards, and is mixed periodically as it passes through the mixing zones where the mixers are located. For a thin slurry, alternate mixing can be achieved by a pumping loop which also serves as a heat transfer device. Additional heat transfer takes place through the reactor heat transfer jackets.

  4. Tower reactors for bioconversion of lignocellulosic material

    DOE Patents [OSTI]

    Nguyen, Quang A.

    1999-01-01

    An apparatus for enzymatic hydrolysis and fermentation of pretreated lignocellulosic material, in the form of a tower bioreactor, having mixers to achieve intermittent mixing of the material. Precise mixing of the material is important for effective heat and mass transfer requirements without damaging or denaturing the enzymes or fermenting microorganisms. The pretreated material, generally in the form of a slurry, is pumped through the bioreactor, either upwards or downwards, and is mixed periodically as it passes through the mixing zones where the mixers are located. For a thin slurry, alternate mixing can be achieved by a pumping loop which also serves as a heat transfer device. Additional heat transfer takes place through the reactor heat transfer jackets.

  5. Tower reactors for bioconversion of lignocellulosic material

    DOE Patents [OSTI]

    Nguyen, Q.A.

    1998-03-31

    An apparatus is disclosed for enzymatic hydrolysis and fermentation of pretreated lignocellulosic material. The apparatus consists of a tower bioreactor which has mixers to achieve intermittent mixing of the material. Precise mixing of the material is important for effective heat and mass transfer requirements without damaging or denaturing the enzymes or fermenting microorganisms. The pretreated material, generally in the form of a slurry, is pumped through the bioreactor, either upwards or downwards, and is mixed periodically as it passes through the mixing zones where the mixers are located. For a thin slurry, alternate mixing can be achieved by a pumping loop which also serves as a heat transfer device. Additional heat transfer takes place through the reactor heat transfer jackets. 5 figs.

  6. Tower reactors for bioconversion of lignocellulosic material

    DOE Patents [OSTI]

    Nguyen, Q.A.

    1999-03-30

    An apparatus is described for enzymatic hydrolysis and fermentation of pretreated lignocellulosic material, in the form of a tower bioreactor, having mixers to achieve intermittent mixing of the material. Precise mixing of the material is important for effective heat and mass transfer requirements without damaging or denaturing the enzymes or fermenting microorganisms. The pretreated material, generally in the form of a slurry, is pumped through the bioreactor, either upwards or downwards, and is mixed periodically as it passes through the mixing zones where the mixers are located. For a thin slurry, alternate mixing can be achieved by a pumping loop which also serves as a heat transfer device. Additional heat transfer takes place through the reactor heat transfer jackets. 5 figs.

  7. (Biotechnology for the conversion of lignocellulosics)

    SciTech Connect (OSTI)

    Woodward, J.

    1990-10-25

    This report summarizes the results of the traveler's participation in the International Energy Agency (IEA) Network planning meeting for Biotechnology for the Conversion of Lignocellulosics,'' held at the Institut Francais du Petrole (IFP), Rueil-Malmaison, France. It also summarizes the results of discussions held at Aston University, Birmingham, UK, with Dr. Martin Beevers with whom the traveler is attempting to initiate a collaborative research project that will be beneficial to ongoing research programs at Oak Ridge National Laboratory (ORNL). The itinerary for the trip is given in Appendix A; the names of the people contacted are listed in Appendix B. Also, pertinent information about the Institut Francais du Petrole is attached (Appendix C). 1 tab.

  8. Process Design and Economics for the Conversion of Lignocellulosic Biomass

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons | Department of Energy the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological

  9. Elucidating the Role of Ferrous Ion Cocatalyst in Enhancing Dilute Acid Pretreatment of Lignocellulosic Biomass

    SciTech Connect (OSTI)

    Wei, H.; Donohoe, B. S.; Vinzant, T. B.; Ciesielski, P. N.; Wang, W.; Gedvilas, L. M.; Zeng, Y.; Johnson, D. K.; Ding, S. Y.; Himmel, M. E.; Tucker, M. P.

    2011-01-01

    Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is known about the underlying mechanisms of this enhancement. In the current study, our aim was to identify several essential factors that contribute to ferrous ion-enhanced efficiency during dilute acid pretreatment of biomass and to initiate the investigation of the mechanisms that result in this enhancement. During dilute acid and ferrous ion cocatalyst pretreatments, we observed concomitant increases in solubilized sugars in the hydrolysate and reducing sugars in the (insoluble) biomass residues. We also observed enhancements in sugar release during subsequent enzymatic saccharification of iron cocatalyst-pretreated biomass. Fourier transform Raman spectroscopy showed that major peaks representing the C-O-C and C-H bonds in cellulose are significantly attenuated by iron cocatalyst pretreatment. Imaging using Prussian blue staining indicated that Fe{sup 2+} ions associate with both cellulose/xylan and lignin in untreated as well as dilute acid/Fe{sup 2+} ion-pretreated corn stover samples. Analyses by scanning electron microscopy and transmission electron microscopy revealed structural details of biomass after dilute acid/Fe{sup 2+} ion pretreatment, in which delamination and fibrillation of the cell wall were observed. By using this multimodal approach, we have revealed that (1) acid-ferrous ion-assisted pretreatment increases solubilization and enzymatic digestion of both cellulose and xylan to monomers and (2) this pretreatment likely targets multiple chemistries in plant cell wall polymer networks, including those represented by the C-O-C and C-H bonds in cellulose.

  10. Genetic manipulation of lignin reduces recalcitrance and improves biomass ethanol production from switchgrass

    SciTech Connect (OSTI)

    Hamilton, Choo Yieng; Fu, Chunxiang; Xiao, Xirong; Ge, Yaxin; Chen, Fang; Bouton, Joseph; Foston, Marcus; Dixon, Richard A; Wang, Zeng-Yu; Mielenz, Jonathan R

    2011-01-01

    Switchgrass is a leading dedicated bioenergy feedstock because it is a native, high yielding, perennial prairie grass with broad cultivation range and low agronomic input requirements. Biomass conversion research has developed pilot scale processes for production of ethanol and other alcohols but they remain costly primarily due to the intrinsic recalcitrance of biomass. We show here that switchgrass genetic modification can produce normal plants that have reduced thermochemical and enzymatic recalcitrance. Downregulation of the switchgrass caffeic O-methyltransferase gene decreases lignin content modestly, reduces the syringyl to guaiacyl lignin monomer ratio and increases the ethanol yield by up to a third using conventional biomass fermentation processes. The downregulated lines have wild-type biomass yields but require reduced pretreatment severity and 300-400% lower cellulase dosages for equivalent product yields significantly lowering processing costs. Alternately, our modified transgenic switchgrass lines should yield significantly more fermentation chemicals per hectare under identical process conditions.

  11. Production of Succinic Acid for Lignocellulosic Hydrolysates

    SciTech Connect (OSTI)

    Davison, B.H.; Nghiem, J.

    2002-06-01

    The purpose of this Cooperative Research and Development Agreement (CRADA) is to add and test new metabolic activities to existing microbial catalysts for the production of succinic acid from renewables. In particular, they seek to add to the existing organism the ability to utilize xylose efficiently and simultaneously with glucose in mixtures of sugars or to add succinic acid production to another strain and to test the value of this new capability for production of succinic acid from industrial lignocellulosic hydrolyasates. The Contractors and Participant are hereinafter jointly referred to as the 'Parties'. Research to date in succinic acid fermentation, separation and genetic engineering has resulted in a potentially economical process based on the use of an Escherichia coli strain AFP111 with suitable characteristics for the production of succinic acid from glucose. Economic analysis has shown that higher value commodity chemicals can be economically produced from succinic acid based on repliminary laboratory findings and predicted catalytic parameters. The initial target markets include succinic acid itself, succinate salts, esters and other derivatives for use as deicers, solvents and acidulants. The other commodity products from the succinic acid platform include 1,4-butanediol, {gamma}-butyrolactone, 2-pyrrolidinone and N-methyl pyrrolidinone. Current economic analyses indicate that this platform is competitive with existing petrochemical routes, especially for the succinic acid and derivatives. The report presents the planned CRADA objectives followed by the results. The results section has a combined biocatalysis and fermentation section and a commercialization section. This is a nonproprietary report; additional proprietary information may be made available subject to acceptance of the appropriate proprietary information agreements.

  12. Differential growth responses of soil bacterial taxa to carbon substrates of varying chemical recalcitrance

    SciTech Connect (OSTI)

    Goldfarb, K.C.; Karaoz, U.; Hanson, C.A.; Santee, C.A.; Bradford, M.A.; Treseder, K.K.; Wallenstein, M.D.; Brodie, E.L.

    2011-04-18

    Soils are immensely diverse microbial habitats with thousands of co-existing bacterial, archaeal, and fungal species. Across broad spatial scales, factors such as pH and soil moisture appear to determine the diversity and structure of soil bacterial communities. Within any one site however, bacterial taxon diversity is high and factors maintaining this diversity are poorly resolved. Candidate factors include organic substrate availability and chemical recalcitrance, and given that they appear to structure bacterial communities at the phylum level, we examine whether these factors might structure bacterial communities at finer levels of taxonomic resolution. Analyzing 16S rRNA gene composition of nucleotide analog-labeled DNA by PhyloChip microarrays, we compare relative growth rates on organic substrates of increasing chemical recalcitrance of >2,200 bacterial taxa across 43 divisions/phyla. Taxa that increase in relative abundance with labile organic substrates (i.e., glycine, sucrose) are numerous (>500), phylogenetically clustered, and occur predominantly in two phyla (Proteobacteria and Actinobacteria) including orders Actinomycetales, Enterobacteriales, Burkholderiales, Rhodocyclales, Alteromonadales, and Pseudomonadales. Taxa increasing in relative abundance with more chemically recalcitrant substrates (i.e., cellulose, lignin, or tannin-protein) are fewer (168) but more phylogenetically dispersed, occurring across eight phyla and including Clostridiales, Sphingomonadalaes, Desulfovibrionales. Just over 6% of detected taxa, including many Burkholderiales increase in relative abundance with both labile and chemically recalcitrant substrates. Estimates of median rRNA copy number per genome of responding taxa demonstrate that these patterns are broadly consistent with bacterial growth strategies. Taken together, these data suggest that changes in availability of intrinsically labile substrates may result in predictable shifts in soil bacterial composition.

  13. Investigating Commercial Cellulase Performances Toward Specific Biomass Recalcitrance Factors Using Reference Substrates

    SciTech Connect (OSTI)

    Ju, Xiaohui; Bowden, Mark E.; Engelhard, Mark H.; Zhang, Xiao

    2014-04-01

    Three commercial cellulase preparations, Novozymes Cellic® Ctec2, Dupont Accellerase® 1500, and DSM Cytolase CL, were evaluated for their hydrolytic activity using a set of reference biomass substrates with controlled substrate characteristics. It was found that lignin remains a significant recalcitrance factor to all the preparations, although different enzyme preparations respond to the inhibitory effect of lignin differently. Also, different types of biomass lignin can inhibit cellulose enzymes in different manners. Enhancing enzyme activity toward biomass fiber swelling is an area significantly contributing to potential improvement in cellulose performance. While the degree of polymerization of cellulose in the reference substrates did not present a major recalcitrance factor to Novozymes Cellic® Ctec2, cellulose crystallite has been shown to have a significant lower reactivity toward all enzyme mixtures. The presence of polysaccharide monooxygenases (PMOs) in Novozymes Ctec2 appears to enhance enzyme activity toward decrystallization of cellulose. This study demonstrated that reference substrates with controlled chemical and physical characteristics of structural features can be applied as an effective and practical strategy to identify cellulosic enzyme activities toward specific biomass recalcitrance factor(s) and provide specific targets for enzyme improvement.

  14. Molecular Siganture and Sources of Biochemical Recalcitrance of Organic C in Amozonian Dark Earths

    SciTech Connect (OSTI)

    Solomon,D.; Lehmann, J.; Thies, J.; Schafer, T.; Liang, B.; Kinyangi, J.; Neves, E.; Peterson, J.; Liuzao, F.; Skjemstad, J.

    2007-01-01

    Amazonian Dark Earths (ADE) are a unique type of soils developed through intense anthropogenic activities that transformed the original soils into Anthrosols throughout the Brazilian Amazon Basin. We conducted a comparative molecular-level investigation of soil organic C (SOC) speciation in ADE (ages between 600 and 8700 years B.P.) and adjacent soils using ultraviolet photo-oxidation coupled with {sup 13}C cross polarization-magic angle spinning nuclear magnetic resonance (CP-MAS NMR), synchrotron-based Fourier transform infrared-attenuated total reflectance (Sr-FTIR-ATR) and C (1s) near edge X-ray absorption fine structure (NEXAFS) spectroscopy to obtain deeper insights into the structural chemistry and sources of refractory organic C compounds in ADE. Our results show that the functional group chemistry of SOC in ADE was considerably different from adjacent soils. The SOC in ADE was enriched with: (i) aromatic-C structures mostly from H- and C-substituted aryl-C, (ii) O-rich organic C forms from carboxylic-C, aldehyde-C, ketonic-C and quinine-C, and (iii) diverse group of refractory aliphatic-C moieties. The SOC in adjacent soils was predominantly composed of O-alkyl-C and methoxyl-C/N-alkyl-C structures and elements of labile aliphatic-C functionalities. Our study suggests that the inherent molecular structures of organic C due to selective accumulation of highly refractory aryl-C structures seems to be the key factor for the biochemical recalcitrance and stability of SOC in ADE. Anthropogenic enrichment with charred carbonaceous residues from biomass-derived black C (BC) is presumed to be the precursor of these recalcitrant polyaromatic structures. Our results also highlight the complementary role that might be played by organic C compounds composed of O-containing organic C moieties and aliphatic-C structures that persisted for millennia in these anthropic soils as additional or secondary sources of chemical recalcitrance of SOC in ADE. These organic C

  15. FRACTIONATION OF LIGNOCELLULOSIC BIOMASS FOR FUEL-GRADE ETHANOL PRODUCTION

    SciTech Connect (OSTI)

    F.D. Guffey; R.C. Wingerson

    2002-10-01

    PureVision Technology, Inc. (PureVision) of Fort Lupton, Colorado is developing a process for the conversion of lignocellulosic biomass into fuel-grade ethanol and specialty chemicals in order to enhance national energy security, rural economies, and environmental quality. Lignocellulosic-containing plants are those types of biomass that include wood, agricultural residues, and paper wastes. Lignocellulose is composed of the biopolymers cellulose, hemicellulose, and lignin. Cellulose, a polymer of glucose, is the component in lignocellulose that has potential for the production of fuel-grade ethanol by direct fermentation of the glucose. However, enzymatic hydrolysis of lignocellulose and raw cellulose into glucose is hindered by the presence of lignin. The cellulase enzyme, which hydrolyzes cellulose to glucose, becomes irreversibly bound to lignin. This requires using the enzyme in reagent quantities rather than in catalytic concentration. The extensive use of this enzyme is expensive and adversely affects the economics of ethanol production. PureVision has approached this problem by developing a biomass fractionator to pretreat the lignocellulose to yield a highly pure cellulose fraction. The biomass fractionator is based on sequentially treating the biomass with hot water, hot alkaline solutions, and polishing the cellulose fraction with a wet alkaline oxidation step. In September 2001 PureVision and Western Research Institute (WRI) initiated a jointly sponsored research project with the U.S. Department of Energy (DOE) to evaluate their pretreatment technology, develop an understanding of the chemistry, and provide the data required to design and fabricate a one- to two-ton/day pilot-scale unit. The efforts during the first year of this program completed the design, fabrication, and shakedown of a bench-scale reactor system and evaluated the fractionation of corn stover. The results from the evaluation of corn stover have shown that water hydrolysis prior to

  16. Understanding Free and Complexed Enzyme Mechanisms and Factors Contributing to Cell Wall Recalcitrance (Presentation)

    SciTech Connect (OSTI)

    Resch, M.; Donohoe, B.; Katahira, R.; Ashutosh, M.; Beckham, G.; Himmel, M.; Decker, S.

    2014-04-01

    Fungal free enzymes and bacterial complexed cellulosomes deconstruct biomass using different physical mechanisms. Free enzymes, which typically contain a large proportion of GH7 cellobiohydrolase, diffuse throughout the substrate and hydrolyze primarily from the cellulose reducing end, resulting in 'sharpened' macrofibrils. In contrast, complexed cellulosomes contain a diverse array of carbohydrate binding modules and multiple catalytic specificities leading to delamination and physical peeling of the cellulose macrofibril structures. To investigate how cellulose structure contributes to recalcitrance, we compared the deconstruction of cellulose I, II, and III; using free and complexed enzyme systems. We also evaluated both systems on Clean Fractionation and alkaline pretreated biomass, which remove much of the lignin, to determine the impact on enzyme loading reduction. Free fungal enzymes demonstrated a swelling of the outer surface of the plant cell walls while removing localized disruptions, resulting in a smooth surface appearance. Cellulosomes produced cell wall surfaces with localized areas of disruption and little surface layer swelling. These studies contribute to the overall understanding of biomass recalcitrance and how combining different enzymatic paradigms may lead to the formulation of new enzyme cocktails to reduce the cost of producing sugars from plant cell wall carbohydrates.

  17. Flow-through biological conversion of lignocellulosic biomass

    DOE Patents [OSTI]

    Herring, Christopher D.; Liu, Chaogang; Bardsley, John

    2014-07-01

    The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

  18. Comparative Study of Corn Stover Pretreated by Dilute Acid and Cellulose Solvent-Based Lignocellulose Fractionation: Enzymatic Hydrolysis, Supramolecular Structure, and Substrate Accessibility

    SciTech Connect (OSTI)

    Zhu, Z.; Sathitsuksanoh, N.; Vinzant, T.; Schell, D. J.; McMillian, J. D.; Zhang, Y. H. P.

    2009-07-01

    Liberation of fermentable sugars from recalcitrant biomass is among the most costly steps for emerging cellulosic ethanol production. Here we compared two pretreatment methods (dilute acid, DA, and cellulose solvent and organic solvent lignocellulose fractionation, COSLIF) for corn stover. At a high cellulase loading [15 filter paper units (FPUs) or 12.3 mg cellulase per gram of glucan], glucan digestibilities of the corn stover pretreated by DA and COSLIF were 84% at hour 72 and 97% at hour 24, respectively. At a low cellulase loading (5 FPUs per gram of glucan), digestibility remained as high as 93% at hour 24 for the COSLIF-pretreated corn stover but reached only {approx}60% for the DA-pretreated biomass. Quantitative determinations of total substrate accessibility to cellulase (TSAC), cellulose accessibility to cellulase (CAC), and non-cellulose accessibility to cellulase (NCAC) based on adsorption of a non-hydrolytic recombinant protein TGC were measured for the first time. The COSLIF-pretreated corn stover had a CAC of 11.57 m{sup 2}/g, nearly twice that of the DA-pretreated biomass (5.89 m{sup 2}/g). These results, along with scanning electron microscopy images showing dramatic structural differences between the DA- and COSLIF-pretreated samples, suggest that COSLIF treatment disrupts microfibrillar structures within biomass while DA treatment mainly removes hemicellulose. Under the tested conditions COSLIF treatment breaks down lignocellulose structure more extensively than DA treatment, producing a more enzymatically reactive material with a higher CAC accompanied by faster hydrolysis rates and higher enzymatic digestibility.

  19. Conversion of Lignocellulosic Biomass to Ethanol Butyl Acrylate

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Conversion of Lignocellulosic Biomass to Ethanol and Butyl Acrylate March 25, 2015 Principal Investigator Thomas P. Binder ARCHER DANIELS MIDLAND COMPANY 2 Where does ADM fit with the IBR? * Ensuring a supply of technology for future growth is a priority for ADM Research * Corn stover utilization may enable continued growth in starch supply while starting a new industry around a currently underutilized material James R Randall Research Center Decatur, IL ARCHER DANIELS MIDLAND COMPANY 3 Quad

  20. Method to Produce Highly Digestible, Pretreated Lignocellulosic Biomass

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Using Anhydrous Liquid Ammonia - Energy Innovation Portal Method to Produce Highly Digestible, Pretreated Lignocellulosic Biomass Using Anhydrous Liquid Ammonia Inventors: Shishir Chundawat, Leonardo Sousa, Albert Cheh, Venkatesh Balan, Bruce Dale Great Lakes Bioenergy Research Center Contact GLBRC About This Technology Technology Marketing SummaryIn the continuing push to develop alternative fuels, bioethanol is clearly a viable option. However, if it is to become a truly economical

  1. Nanoporous Membranes for Pretreatment of Lignocellulose and Other

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Applications - Energy Innovation Portal Industrial Technologies Industrial Technologies Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Nanoporous Membranes for Pretreatment of Lignocellulose and Other Applications Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication Final 12-G00208_2406.pdf (1,333 KB) Technology Marketing SummaryResearchers at ORNL have developed an

  2. CLSF (Center for Lignocellulose Structure and Formation) - About the Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    About the Center EFRCs (Energy Frontier Research Centers) The Center for Lignocellulose Structure and Formation (CLSF) is one of 46 Energy Frontier Research Centers (EFRC) initially established in 2009 by the US Department of Energy to accelerate research to meet critical energy challenges of the 21st century. EFRCs integrate the expertise of multiple leading scientific investigators to enable fundamental research of a scope and complexity that would not be possible with the small group research

  3. Lignocellulose Degradation Mechanisms Across the Tree of Life

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cragg, Simon M.; Beckham, Gregg T.; Bruce, Neil C.; Bugg, Timothy D. H.; Distel, Daniel L.; Dupree, Paul; Etxabe, Amaia Green; Goodell, Barry S.; Jellison, Jody; McGeehan, John E.; et al

    2015-11-14

    Organisms use diverse mechanisms involving multiple complementary enzymes, particularly glycoside hydrolases (GHs), to deconstruct lignocellulose. Lytic polysaccharide monooxygenases (LPMOs) produced by bacteria and fungi facilitate deconstruction as does the Fenton chemistry of brown-rot fungi. Lignin depolymerisation is achieved by white-rot fungi and certain bacteria, using peroxidases and laccases. Meta-omics is now revealing the complexity of prokaryotic degradative activity in lignocellulose-rich environments. Protists from termite guts and some oomycetes produce multiple lignocellulolytic enzymes. We found that the Lignocellulose-consuming animals secrete some GHs, but most harbour a diverse enzyme-secreting gut microflora in a mutualism that is particularly complex in termites. Shipworms however,more » house GH-secreting and LPMO-secreting bacteria separate from the site of digestion and the isopod Limnoria relies on endogenous enzymes alone. Moreover, the omics revolution is identifying many novel enzymes and paradigms for biomass deconstruction, but more emphasis on function is required, particularly for enzyme cocktails, in which LPMOs may play an important role.« less

  4. Lignocellulose Degradation Mechanisms Across the Tree of Life

    SciTech Connect (OSTI)

    Cragg, Simon M.; Beckham, Gregg T.; Bruce, Neil C.; Bugg, Timothy D. H.; Distel, Daniel L.; Dupree, Paul; Etxabe, Amaia Green; Goodell, Barry S.; Jellison, Jody; McGeehan, John E.; McQueen-Mason, Simon J.; Schnorr, Kirk; Walton, Paul H.; Watts, Joy E. M.; Zimmer, Martin

    2015-11-14

    Organisms use diverse mechanisms involving multiple complementary enzymes, particularly glycoside hydrolases (GHs), to deconstruct lignocellulose. Lytic polysaccharide monooxygenases (LPMOs) produced by bacteria and fungi facilitate deconstruction as does the Fenton chemistry of brown-rot fungi. Lignin depolymerisation is achieved by white-rot fungi and certain bacteria, using peroxidases and laccases. Meta-omics is now revealing the complexity of prokaryotic degradative activity in lignocellulose-rich environments. Protists from termite guts and some oomycetes produce multiple lignocellulolytic enzymes. We found that the Lignocellulose-consuming animals secrete some GHs, but most harbour a diverse enzyme-secreting gut microflora in a mutualism that is particularly complex in termites. Shipworms however, house GH-secreting and LPMO-secreting bacteria separate from the site of digestion and the isopod Limnoria relies on endogenous enzymes alone. Moreover, the omics revolution is identifying many novel enzymes and paradigms for biomass deconstruction, but more emphasis on function is required, particularly for enzyme cocktails, in which LPMOs may play an important role.

  5. Method for the delignification of lignocellulosic material by adding a dialkyl substituted octahydroanthraquinone

    DOE Patents [OSTI]

    Dimmel, Donald R.

    2000-01-01

    The present invention provides a method for the synthesis of substituted octahydroanthraquinones and substituted anthraquinones which are effective for pulping of lignocellulosics.

  6. Pentose fermentation of normally toxic lignocellulose prehydrolysate with strain of Pichia stipitis yeast using air

    DOE Patents [OSTI]

    Keller, Jr., Fred A.; Nguyen, Quang A.

    2002-01-01

    Strains of the yeast Pichia stipitis NPw9 (ATCC PTA-3717) useful for the production of ethanol using oxygen for growth while fermenting normally toxic lignocellulosic prehydrolysates.

  7. Medical Screening

    Broader source: Energy.gov [DOE]

    Medical Screening: Provide medical screening exams that are designed to check for health conditions related to occupational exposures to former workers who choose to participate in the program, including a re-screen exam every three years.

  8. Conversion of Lignocellulosic Biomass to Ethanol and Butyl Acrylate

    SciTech Connect (OSTI)

    Binder, Thomas; Erpelding, Michael; Schmid, Josef; Chin, Andrew; Sammons, Rhea; Rockafellow, Erin

    2015-04-10

    Conversion of Lignocellulosic Biomass to Ethanol and Butyl Acrylate. The purpose of Archer Daniels Midlands Integrated Biorefinery (IBR) was to demonstrate a modified acetosolv process on corn stover. It would show the fractionation of crop residue to distinct fractions of cellulose, hemicellulose, and lignin. The cellulose and hemicellulose fractions would be further converted to ethanol as the primary product and a fraction of the sugars would be catalytically converted to acrylic acid, with butyl acrylate the final product. These primary steps have been demonstrated.

  9. Study of lignocellulose components for production of lactic acid

    SciTech Connect (OSTI)

    Padukone, N.; Schmidt, S.L.; Goodman, B.J.; Wyman, C.E.

    1993-12-31

    Lactic acid promises to be an important chemical feedstock in the future for the production of biodegradable and biocompatible polymers. About half of the current US consumption is imported to meet the escalating demand from both the food and chemical industries. The potential future market for polylactide products would further stress the domestic capacity of lactic acid production. Renewable resources such as lignocellulosic crops and wastes are abundant and could be utilized for the production of important fuels and chemicals. This would not only reduce our dependence on limited reserves of fossil fuels but also alleviate the environmental burden of waste accumulation and disposal.

  10. On using rational enzyme redesign to improve enzyme-mediated microbial dehalogenation of recalcitrant substances in deep-subsurface environments

    SciTech Connect (OSTI)

    Ornstein, R.L.

    1993-06-01

    Heavily halogenated hydrocarbons are one of the most prevalent classes of man-made recalcitrant environmental contaminants and often make their way into subsurface environments. Biodegradation of heavily chlorinated compounds in the deep subsurface often occurs at extremely slow rates because native enzymes of indigenous microbes are unable to efficiently metabolize such synthetic substances. Cost-effective engineering solutions do not exist for dealing with disperse and recalcitrant pollutants in the deep subsurface (i.e., ground water, soils, and sediments). Timely biodegradation of heavily chlorinated compounds in the deep subsurface may be best accomplished by rational redesign of appropriate enzymes that enhance the ability of indigenous microbes to metabolize these substances. The isozyme family cytochromes P450 are catalytically very robust and are found in all aerobic life forms and may be active in may anaerobes as well. The author is attempting to demonstrate proof-of-principle rational enzyme redesign of cytochromes P450 to enhance biodehalogenation.

  11. New Innovations in Highly Ion Specific Media for Recalcitrant Waste stream Radioisotopes

    SciTech Connect (OSTI)

    Denton, M. S.; Wilson, J.; Ahrendt, M. [RWE NUKEM Corporation (RNC), 800 Oak Ridge Tnpk., Suite A701, Oak Ridge, TN 37830 (United States); Bostick, W. D. [Materials and Chemistry Laboratory (MCL), Inc., East Tennessee Technology Park, Building K-1006, 2010 Highway 58, Suite 1000, Oak Ridge, TN 37830 (United States); DeSilva, F.; Meyers, P. [ResinTech, Inc., 1 ResinTech Plaza, 160 Cooper Road, West Berlin, NJ 08091 (United States)

    2006-07-01

    Specialty ion specific media were examined and developed for, not only pre- and post-outage waste streams, but also for very difficult outage waste streams. This work was carried out on first surrogate waste streams, then laboratory samples of actual waste streams, and, finally, actual on-site waste streams. This study was particularly focused on PWR wastewaters such as Floor Drain Tank (FDT), Boron Waste Storage Tank (BWST), and Waste Treatment Tank (WTT, or discharge tank). Over the last half decade, or so, treatment technologies have so greatly improved and discharge levels have become so low, that certain particularly problematic isotopes, recalcitrant to current treatment skids, are all that remain prior to discharge. In reality, they have always been present, but overshadowed by the more prevalent and higher activity isotopes. Such recalcitrants include cobalt, especially Co 58 [both ionic/soluble (total dissolved solids, TDS) and colloidal (total suspended solids, TSS)] and antimony (Sb). The former is present in most FDT and BWST wastewaters, while the Sb is primarily present in BWST waste streams. The reasons Co 58 can be elusive to granulated activated carbon (GAC), ultrafiltration (UF) and ion exchange (IX) demineralizers is that it forms submicron colloids as well as has a tendency to form metal complexes with chelating agents (e.g., ethylene diamine tetraacetic acid, or EDTA). Such colloids and non-charged complexes will pass through the entire treatment skid. Antimony (Sb) on the other hand, has little or no ionic charge, and will, likewise, pass through both the filtration and de-min skids into the discharge tanks. While the latter will sometimes (the anionic vs. the cationic or neutral species) be removed on the anion bed(s), it will slough off (snow-plow effect) when a higher affinity anion (iodine slugs, etc.) comes along; thus causing effluents not meeting discharge criteria. The answer to these problems found in this study, during an actual

  12. Center for Lignocellulose Structure and Formation (CLSF) | U.S. DOE Office

    Office of Science (SC) Website

    of Science (SC) Lignocellulose Structure and Formation (CLSF) Energy Frontier Research Centers (EFRCs) EFRCs Home Centers EFRC External Websites Research Science Highlights News & Events Publications History Contact BES Home Centers Center for Lignocellulose Structure and Formation (CLSF) Print Text Size: A A A FeedbackShare Page CLSF Header Director Daniel Cosgrove Lead Institution Pennsylvania State University Year Established 2009 Mission To develop a nano- to meso-scale understanding

  13. Revealing the Differences Between Free and Complexed Enzyme Mechanisms and Factors Contributing to Cell Wall Recalcitrance

    SciTech Connect (OSTI)

    Resch, M.

    2014-09-08

    Enzymatic depolymerization of polysaccharides is a key step in the production of fuels and chemicals from lignocellulosic biomass, and discovery of synergistic biomass-degrading enzyme paradigms will enable improved conversion processes. Historically, revealing insights into enzymatic saccharification mechanisms on plant cell walls has been hindered by uncharacterized substrates and low resolution imaging techniques. Also, translating findings between model substrates to intact biomass is critical for evaluating enzyme performance. Here we employ a fungal free enzyme cocktail, a complexed cellulosomal system, and a combination of the two to investigate saccharification mechanisms on cellulose I, II and III along with corn stover from Clean Fractionation (CF), which is an Organosolv pretreatment. The insoluble Cellulose Enriched Fraction (CEF) from CF contains mainly cellulose with minor amounts of residual hemicellulose and lignin, the amount of which depends on the CF pretreatment severity. Enzymatic digestions at both low and high-solids loadings demonstrate that CF reduces the amount of enzyme required to depolymerize polysaccharides relative to deacetylated, dilute acid pretreated corn stover. Transmission and scanning electron microscopy of the biomass provides evidence for the different mechanisms of enzymatic deconstruction between free and complexed enzyme systems, and reveals the basis for the synergistic relationship between the two enzyme paradigms on a process-relevant substrate for the first time. These results also demonstrate that the presence of lignin, rather than cellulose morphology, is more detrimental to cellulosome action than to free cellulases. As enzyme costs are a major economic driver for biorefineries, this study provides key inputs for the evaluation of CF as a pretreatment method for biomass conversion.

  14. Preliminary Screening

    Broader source: Energy.gov [DOE]

    The first step in assessing renewable energy options is to conduct a preliminary screening to decide which technologies are worth investigating and which can be eliminated immediately. Preliminary...

  15. Production of ethanol from lignocellulosic materials using thermophilic bacteria

    SciTech Connect (OSTI)

    Lynd, L.R.

    1987-01-01

    The production of ethanol from lignocellulosic materials, e.g. wood, agricultural residues, and municipal solid wastes, is considered. The conversion of these materials to ethanol in the US could annually yield approximately 430 million tons ethanol, or about 9.8 quads, within the next 20 years. Thermophilic bacteria have advantages over yeasts for ethanol production because various species produce an active cellulase enzyme and utilize pentose sugars. However thermophiles have lower ethanol tolerance and usually lower ethanol yields. The potential of thermophilic ethanol production from hardwood chips is examined in detail. It is concluded that if high ethanol yield can be achieved this process could have economics competitive with either ethanol production from corn via yeast or synthetic production from ethylene. Low ethanol tolerance is not a major problem provided concentrations {ge} 1.5% are produced, ethanol is continuously removed from the fermentor, and IHOSR/extractive distillation is employed. Research was undertaken aimed at closing the gap between the attractive potential of thermophiles for ethanol production, and that which is possible based on present knowledge, which is not practical. Major topics were the activity of Clostridium thermocellum cellulase on pretreated mixed hardwood and Avicel in vivo, continuous culture of C. thermocellum on pretreated mixed hardwood and Avicel, and the continuous culture of Clostridium thermosaccharolyticum at high xylose concentrations in the presence and absence of ethanol removal.

  16. CONVERSION OF LIGNOCELLULOSIC MATERIAL TO CHEMICALS AND FUELS

    SciTech Connect (OSTI)

    Edwin S. Olson

    2001-06-30

    A direct conversion of cellulosic wastes, including resin-bonded furniture and building waste, to levulinate esters is being investigated with the view to producing fuels, solvents, and chemical intermediates as well as other useful by-products in an inexpensive process. The acid-catalyzed reaction of cellulosic materials with ethanol or methanol at 200 C gives good yields of levulinate and formate esters, as well as useful by-products, such as a solid residue (charcoal) and a resinous lignin residue. An initial plant design showed reasonable rates of return for production of purified ethyl levulinate and by-products. In this project, investigations have been performed to identify and develop reactions that utilize esters of levulinic acid produced during the acid-catalyzed ethanolysis reaction. We wish to develop uses for levulinate esters that allow their marketing at prices comparable to inexpensive polymer intermediates. These prices will allow a sufficient rate of return to justify building plants for utilizing the waste lignocellulosics. If need is demonstrated for purified levulinate, the initial plant design work may be adequate, at least until further pilot-scale work on the process is performed.

  17. Current Challenges in Commercially Producing Biofuels from Lignocellulosic Biomass

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Balan, Venkatesh

    2014-01-01

    Biofuels that are produced from biobased materials are a good alternative to petroleum based fuels. They offer several benefits to society and the environment. Producing second generation biofuels is even more challenging than producing first generation biofuels due the complexity of the biomass and issues related to producing, harvesting, and transporting less dense biomass to centralized biorefineries. In addition to this logistic challenge, other challenges with respect to processing steps in converting biomass to liquid transportation fuel like pretreatment, hydrolysis, microbial fermentation, and fuel separation still exist and are discussed in this review. The possible coproducts that could be producedmore » in the biorefinery and their importance to reduce the processing cost of biofuel are discussed. About $1 billion was spent in the year 2012 by the government agencies in US to meet the mandate to replace 30% existing liquid transportation fuels by 2022 which is 36 billion gallons/year. Other countries in the world have set their own targets to replace petroleum fuel by biofuels. Because of the challenges listed in this review and lack of government policies to create the demand for biofuels, it may take more time for the lignocellulosic biofuels to hit the market place than previously projected.« less

  18. Recalcitrance and structural analysis by water-only flowthrough pretreatment of 13C enriched corn stover stem

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Foston, Marcus B.; Trajanob, Heather L.; Samuel, Reichel; Wyman, Charles E.; He, Jian; Ragauskas, Arthur J.

    2015-08-28

    Here, this study presents high temperature water-only continuous flowthrough pretreatment coupled with nuclear magnetic resonance (NMR) as a promising analytical tool to examine the plant cell wall, to understand its recalcitrance (i.e., cell wall resistance to deconstruction), and to probe the chemistry occurring during batch pretreatment of biomass. 13C-enriched corn stover stems were pretreated at 170 °C for 60 min with a hot-water flow rate of 20 mL/min to control fractionation of the cell wall. This approach helped elucidate the nature of plant cell wall chemical recalcitrance and biomass pretreatment chemistry by tracking cell wall fragmentation as a function ofmore » time. Fractions of the reactor effluent were collected in a time-resolved fashion and characterized by various NMR techniques to determine the degree and sequence of fragments released, as well as, the chemical composition, molecular structure, and relative molecular weight of those released fragments.« less

  19. Solar assisted alkali pretreatment of garden biomass: Effects on lignocellulose degradation, enzymatic hydrolysis, crystallinity and ultra-structural changes in lignocellulose

    SciTech Connect (OSTI)

    Gabhane, Jagdish; William, S.P.M. Prince; Vaidya, Atul N.; Das, Sera; Wate, Satish R.

    2015-06-15

    Highlights: • SAAP is an efficient and economic means of pretreatment. • SAAP was found to be efficient in lignin and hemicellulose removal. • SAAP enhanced the enzymatic hydrolysis. • FTIR, XRD and SEM provided vivid understanding about the mode of action of SAAP. • Mass balance closer of 98% for pretreated GB confirmed the reliability of SAAP. - Abstract: A comprehensive study was carried out to assess the effectiveness of solar assisted alkali pretreatment (SAAP) on garden biomass (GB). The pretreatment efficiency was assessed based on lignocellulose degradation, conversion of cellulose into reducing sugars, changes in the ultra-structure and functional groups of lignocellulose and impact on the crystallinity of cellulose, etc. SAAP was found to be efficient for the removal of lignin and hemicellulose that facilitated enzymatic hydrolysis of cellulose. FTIR and XRD studies provided details on the effectiveness of SAAP on lignocellulosic moiety and crystallinity of cellulose. Scanning electron microscopic analysis showed ultra-structural disturbances in the microfibrils of GB as a result of pretreatment. The mass balance closer of 97.87% after pretreatment confirmed the reliability of SAAP pretreatment. Based on the results, it is concluded that SAAP is not only an efficient means of pretreatment but also economical as it involved no energy expenditure for heat generation during pretreatment.

  20. Determining the cost of producing ethanol from corn starch and lignocellulosic feedstocks

    SciTech Connect (OSTI)

    McAloon, Andrew; Taylor, Frank; Yee, Winnie; Ibsen, Kelly; Wooley, Robert

    2000-10-01

    This report describes the comparison of the processes, each producing 25 million annual gallons of fuel ethanol. This paper attempts to compare the two processes as mature technologies, which requires assuming that the technology improvements needed to make the lignocellulosic process commercializable are achieved, and enough plants have been built to make the design well-understood.

  1. Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol

    SciTech Connect (OSTI)

    2011-05-02

    The U.S. Department of Energy (DOE) promotes the production of ethanol and other liquid fuels from lignocellulosic biomass feedstocks by funding fundamental and applied research that advances the state of technology in biomass collection, conversion, and sustainability. As part of its involvement in the program, the National Renewable Energy Laboratory (NREL) investigates the production economics of these fuels.

  2. Feasibility study for co-locating and integrating ethanol production plants from corn starch and lignocellulosic feedstocks

    SciTech Connect (OSTI)

    Wallace, Robert; Ibsen, Kelly; McAloon, Andrew; Yee, Winnie

    2005-01-01

    Analysis of the feasibility of co-locating corn-grain-to-ethanol and lignocellulosic ethanol plants and potential savings from combining utilities, ethanol purification, product processing, and fermentation.

  3. Membrane contactor/separator for an advanced ozone membrane reactor for treatment of recalcitrant organic pollutants in water

    SciTech Connect (OSTI)

    Chan, Wai Kit; Joueet, Justine; Heng, Samuel; Yeung, King Lun; Schrotter, Jean-Christophe

    2012-05-15

    An advanced ozone membrane reactor that synergistically combines membrane distributor for ozone gas, membrane contactor for pollutant adsorption and reaction, and membrane separator for clean water production is described. The membrane reactor represents an order of magnitude improvement over traditional semibatch reactor design and is capable of complete conversion of recalcitrant endocrine disrupting compounds (EDCs) in water at less than three minutes residence time. Coating the membrane contactor with alumina and hydrotalcite (Mg/Al=3) adsorbs and traps the organics in the reaction zone resulting in 30% increase of total organic carbon (TOC) removal. Large surface area coating that diffuses surface charges from adsorbed polar organic molecules is preferred as it reduces membrane polarization that is detrimental to separation. - Graphical abstract: Advanced ozone membrane reactor synergistically combines membrane distributor for ozone, membrane contactor for sorption and reaction and membrane separator for clean water production to achieve an order of magnitude enhancement in treatment performance compared to traditional ozone reactor. Highlights: Black-Right-Pointing-Pointer Novel reactor using membranes for ozone distributor, reaction contactor and water separator. Black-Right-Pointing-Pointer Designed to achieve an order of magnitude enhancement over traditional reactor. Black-Right-Pointing-Pointer Al{sub 2}O{sub 3} and hydrotalcite coatings capture and trap pollutants giving additional 30% TOC removal. Black-Right-Pointing-Pointer High surface area coating prevents polarization and improves membrane separation and life.

  4. Low Solids Enzymatic Saccharification of Lignocellulosic Biomass: Laboratory Analytical Procedure (LAP), Issue Date: February 4, 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Low Solids Enzymatic Saccharification of Lignocellulosic Biomass Laboratory Analytical Procedure (LAP) Issue Date: February 4, 2015 M. G. Resch, J. O. Baker, and S. R. Decker Technical Report NREL/TP-5100-63351 February 2015 NREL is a national laboratory of the U.S. Department of Energy Office of Energy Efficiency & Renewable Energy Operated by the Alliance for Sustainable Energy, LLC This report is available at no cost from the National Renewable Energy Laboratory (NREL) at

  5. US-India Consortium for Development of Sustainable Advanced Lignocellulosic Biofuels Systems

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    US-India Consortium for Development of Sustainable Advanced Lignocellulosic Biofuels Systems This presentation does not contain any proprietary, confidential, or otherwise restricted information 24 March 2015 Feedstock Supply and Logistics Technology Area Dr. Wilfred Vermerris University of Florida Partner institutions: University of Missouri, Montclair State University, Virginia Tech, Texas A&M University Goal Statement 1. Develop sustainable production systems for switchgrass and biomass

  6. Hydrolyzed Lignocellulose as a Feedstock for Fuels Synthesis Presentation for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Unclassified DOE Bioenergy Technologies Office (BETO) 2015 Project Peer Review FY 14-15: Hydrolyzed Lignocellulose as a Feedstock for Fuels Synthesis 25 th March 2015 Biochemical Conversion PI: John C. Gordon. Co-PI: Andrew D. Sutton Los Alamos National Laboratory Operated by Los Alamos National Security, LLC for the U.S. Department of Energy's NNSA UNCLASSIFIED Unclassified Goal Statement  Use mild conditions and simple catalysts in chemical processes to convert biomass hydrolysates to fuels

  7. Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover

    SciTech Connect (OSTI)

    2002-06-01

    This report is an update of NREL’s ongoing process design and economic analyses of processes related to developing ethanol from lignocellulosic feedstocks.

  8. Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis For Corn Stover

    Office of Energy Efficiency and Renewable Energy (EERE)

    This report is an update of NREL’s ongoing process design and economic analyses of processes related to developing ethanol from lignocellulosic feedstocks.

  9. Old and stable soil organic matter is not necessarily chemically recalcitrant: Implications for modeling concepts and temperature sensitivity

    SciTech Connect (OSTI)

    Kleber, M.; Nico, P.S.; Plante, A.; Filley, T.; Kramer, M.; Swanston, C.; Sollins, P.

    2010-03-01

    complex or 'recalcitrant' compounds will erroneously attribute a greater temperature sensitivity to those materials than they may actually possess.

  10. Groundwater Screen

    Energy Science and Technology Software Center (OSTI)

    1993-11-09

    GWSCREEN was developed for assessment of the groundwater pathway from leaching of radioactive and non radioactive substances from surface or buried sources and release to percolation ponds. The code calculates the limiting soil concentration or effluent release concentration such that, after leaching and transport to the aquifer, regulatory contaminant levels in groundwater are not exceeded. The code uses a mass conservation approach to model three processes: Contaminant release from a source volume, contaminant transport inmore » the unsaturated zone, and contaminant transport in the saturated zone. The source model considers the sorptive properties and solubility of the contaminant. Transport in the unsaturated zone is described by a plug flow model. Transport in the saturated zone is calculated with a semi-analytical solution to the advection dispersion equation in groundwater. Concentration as a function of time at a user specified receptor point and maximum concentration averaged over the exposure interval are also calculated. In addition, the code calculates transport and impacts of radioactive progeny. Input to GWSCREEN is through one, free format ASCII file. This code was designed for assessment and screening of the groundwater pathway when field data is limited. It was not intended to be a predictive tool.« less

  11. Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K.; Gjersing, Erica; Davis, Mark F.; Davison, Brian H.; Olstad, Jessica; Donohoe, Bryon S.; Nguyen, Thanh Yen; Wyman, Charles E.; et al

    2016-01-12

    Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. Thus, to further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.

  12. Deletion of a gene cluster encoding pectin degrading enzymes in Caldicellulosiruptor bescii reveals an important role for pectin in plant biomass recalcitrance

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chung, Daehwan; Pattathil, Sivakumar; Biswal, Ajaya K.; Hahn, Michael G.; Mohnen, Debra; Westpheling, Janet

    2014-10-10

    A major obstacle, and perhaps the most important economic barrier to the effective use of plant biomass for the production of fuels, chemicals, and bioproducts, is our current lack of knowledge of how to efficiently and effectively deconstruct wall polymers for their subsequent use as feedstocks. Plants represent the most desired source of renewable energy and hydrocarbons because they fix CO2, making their use carbon neutral. Their biomass structure, however, is a barrier to deconstruction, and this is often referred to as recalcitrance. Members of the bacterial genus Caldicellulosiruptor have the ability to grow on unpretreated plant biomass and thusmore » provide an assay for plant deconstruction and biomass recalcitrance. Using recently developed genetic tools for manipulation of these bacteria, a deletion of a gene cluster encoding enzymes for pectin degradation was constructed, and the resulting mutant was reduced in its ability to grow on both dicot and grass biomass, but not on soluble sugars. The plant biomass from three phylogenetically diverse plants, Arabidopsis (a herbaceous dicot), switchgrass (a monocot grass), and poplar (a woody dicot), was used in these analyses. These biomass types have cell walls that are significantly different from each other in both structure and composition. While pectin is a relatively minor component of the grass and woody dicot substrates, the reduced growth of the mutant on all three biomass types provides direct evidence that pectin plays an important role in biomass recalcitrance. Glycome profiling of the plant material remaining after growth of the mutant on Arabidopsis biomass compared to the wild-type revealed differences in the rhamnogalacturonan I, homogalacturonan, arabinogalactan, and xylan profiles. In contrast, only minor differences were observed in the glycome profiles of the switchgrass and poplar biomass. In conclusion, the combination of microbial digestion and plant biomass analysis provides a new and

  13. Deletion of a gene cluster encoding pectin degrading enzymes in Caldicellulosiruptor bescii reveals an important role for pectin in plant biomass recalcitrance

    SciTech Connect (OSTI)

    Chung, Daehwan; Pattathil, Sivakumar; Biswal, Ajaya K.; Hahn, Michael G.; Mohnen, Debra; Westpheling, Janet

    2014-10-10

    A major obstacle, and perhaps the most important economic barrier to the effective use of plant biomass for the production of fuels, chemicals, and bioproducts, is our current lack of knowledge of how to efficiently and effectively deconstruct wall polymers for their subsequent use as feedstocks. Plants represent the most desired source of renewable energy and hydrocarbons because they fix CO2, making their use carbon neutral. Their biomass structure, however, is a barrier to deconstruction, and this is often referred to as recalcitrance. Members of the bacterial genus Caldicellulosiruptor have the ability to grow on unpretreated plant biomass and thus provide an assay for plant deconstruction and biomass recalcitrance. Using recently developed genetic tools for manipulation of these bacteria, a deletion of a gene cluster encoding enzymes for pectin degradation was constructed, and the resulting mutant was reduced in its ability to grow on both dicot and grass biomass, but not on soluble sugars. The plant biomass from three phylogenetically diverse plants, Arabidopsis (a herbaceous dicot), switchgrass (a monocot grass), and poplar (a woody dicot), was used in these analyses. These biomass types have cell walls that are significantly different from each other in both structure and composition. While pectin is a relatively minor component of the grass and woody dicot substrates, the reduced growth of the mutant on all three biomass types provides direct evidence that pectin plays an important role in biomass recalcitrance. Glycome profiling of the plant material remaining after growth of the mutant on Arabidopsis biomass compared to the wild-type revealed differences in the rhamnogalacturonan I, homogalacturonan, arabinogalactan, and xylan profiles. In contrast, only minor differences were observed in the glycome profiles of the switchgrass and poplar biomass. In conclusion, the combination of microbial digestion and plant biomass analysis provides a new

  14. Method of separating lignocellulosic material into lignin, cellulose and dissolved sugars

    DOE Patents [OSTI]

    Black, Stuart K.; Hames, Bonnie R.; Myers, Michele D.

    1998-01-01

    A method for separating lignocellulosic material into (a) lignin, (b) cellulose, and (c) hemicellulose and dissolved sugars. Wood or herbaceous biomass is digested at elevated temperature in a single-phase mixture of alcohol, water and a water-immiscible organic solvent (e.g., a ketone). After digestion, the amount of water or organic solvent is adjusted so that there is phase separation. The lignin is present in the organic solvent, the cellulose is present in a solid pulp phase, and the aqueous phase includes hemicellulose and any dissolved sugars.

  15. Method of separating lignocellulosic material into lignin, cellulose and dissolved sugars

    DOE Patents [OSTI]

    Black, S.K.; Hames, B.R.; Myers, M.D.

    1998-03-24

    A method is described for separating lignocellulosic material into (a) lignin, (b) cellulose, and (c) hemicellulose and dissolved sugars. Wood or herbaceous biomass is digested at elevated temperature in a single-phase mixture of alcohol, water and a water-immiscible organic solvent (e.g., a ketone). After digestion, the amount of water or organic solvent is adjusted so that there is phase separation. The lignin is present in the organic solvent, the cellulose is present in a solid pulp phase, and the aqueous phase includes hemicellulose and any dissolved sugars.

  16. Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system

    DOE Patents [OSTI]

    Lu, Jue; Okeke, Benedict

    2012-03-27

    The present invention describes a process for saccharification of lignocelluloses to sugars using whole microbial cells, which are enriched from cultures inoculated with paper mill waste water, wood processing waste and soil. A three-member bacterial consortium is selected as a potent microbial inocula and immobilized on inedible plant fibers for biomass saccharification. The present invention further relates the design of a dual bioreactor system, with various biocarriers for enzyme immobilization and repeated use. Sugars are continuously removed eliminating end-product inhibition and consumption by cell.

  17. Plasma Screen Floating Mount

    DOE Patents [OSTI]

    Eakle, Robert F.; Pak, Donald J.

    2004-10-26

    A mounting system for a flat display screen, particularly a plasma display screen, suspends the screen separately in each of the x-, y- and z-directions. A series of frames located by linear bearings and isolated by springs and dampers allows separate controlled movement in each axis. The system enables the use of relatively larger display screens in vehicles in which plasma screen are subject to damage from vibration.

  18. Outlook for cellulase improvement: Screening and selection strategies

    SciTech Connect (OSTI)

    Zhang, Yiheng P; Himmel, Michael; Mielenz, Jonathan R

    2006-03-01

    Cellulose is the most abundant renewable natural biological resource, and the production of biobased products and bioenergy from less costly renewable lignocellulosic materials is important for the sustainable development of human beings. A reduction in cellulase production cost, an improvement in cellulase performance, and an increase in sugar yields are all vital to reduce the processing costs of biorefineries. Improvements in specific cellulase activities for non-complexed cellulase mixtures can be implemented through cellulase engineering based on rational design or directed evolution for each cellulase component enzyme, as well as on the reconstitution of cellulase components. Here, we review quantitative cellulase activity assays using soluble and insoluble substrates, and focus on their advantages and limitations. Because there are no clear relationships between cellulase activities on soluble substrates and those on insoluble substrates, soluble substrates should not be used to screen or select improved cellulases for processing relevant solid substrates, such as plant cell walls. Cellulase improvement strategies based on directed evolution using screening on soluble substrates have been only moderately successful, and have primarily targeted improvement in thermal tolerance. Heterogeneity of insoluble cellulose, unclear dynamic interactions between insoluble substrate and cellulase components, and the complex competitive and/or synergic relationship among cellulase components limit rational design and/or strategies, depending on activity screening approaches. Herein, we hypothesize that continuous culture using insoluble cellulosic substrates could be a powerful selection tool for enriching beneficial cellulase mutants from the large library displayed on the cell surface.

  19. Preventive Health Screenings

    Broader source: Energy.gov [DOE]

    Memo contains examples of screenings that are eligible for 4 hours of excused absence each leave year

  20. Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Biswal, Ajaya K.; Hao, Zhangying; Pattathil, Sivakumar; Yang, Xiaohan; Winkeler, Kim; Collins, Cassandra; Mohanty, Sushree S.; Richardson, Elizabeth A.; Gelineo-Albersheim, Ivana; Hunt, Kimberly; et al

    2015-03-12

    The inherent recalcitrance of woody bioenergy feedstocks is a major challenge for their use as a source of second-generation biofuel. Secondary cell walls that constitute the majority of hardwood biomass are rich in cellulose, xylan, and lignin. The interactions among these polymers prevent facile accessibility and deconstruction by enzymes and chemicals. Plant biomass that can with minimal pretreatment be degraded into sugars is required to produce renewable biofuels in a cost-effective manner. The following are the results: GAUT12/IRX8 is a putative glycosyltransferase proposed to be involved in secondary cell wall glucuronoxylan and/or pectin biosynthesis based on concomitant reductions of bothmore » xylan and the pectin homogalacturonan (HG) in Arabidopsis irx8 mutants. Two GAUT12 homologs exist in Populus trichocarpa, PtGAUT12.1 and PtGAUT12.2. Knockdown expression of both genes simultaneously has been shown to reduce xylan content in Populus wood. We tested the proposition that RNA interference (RNAi) downregulation of GAUT12.1 alone would lead to increased sugar release in Populus wood, that is, reduced recalcitrance, based on the hypothesis that GAUT12 synthesizes a wall structure required for deposition of xylan and that cell walls with less xylan and/or modified cell wall architecture would have reduced recalcitrance. Using an RNAi approach, we generated 11 Populus deltoides transgenic lines with 50 to 67% reduced PdGAUT12.1 transcript expression compared to wild type (WT) and vector controls. Ten of the eleven RNAi lines yielded 4 to 8% greater glucose release upon enzymatic saccharification than the controls. The PdGAUT12.1 knockdown (PdGAUT12.1-KD) lines also displayed 12 to 52% and 12 to 44% increased plant height and radial stem diameter, respectively, compared to the controls. Knockdown of PdGAUT12.1 resulted in a 25 to 47% reduction in galacturonic acid and 17 to 30% reduction in xylose without affecting total lignin content, revealing that in

  1. Technoeconomic Analysis of a Lignocellulosic Biomass Indirect Gasification Process to Make Ethanol via Mixed Alcohols Synthesis

    SciTech Connect (OSTI)

    Phillips, S. D.

    2007-01-01

    A technoeconomic analysis of a 2000 tonne/day lignocellulosic biomass conversion process to make mixed alcohols via gasification and catalytic synthesis was completed. The process, modeled using ASPEN Plus process modeling software for mass and energy calculations, included all major process steps to convert biomass into liquid fuels, including gasification, gas cleanup and conditioning, synthesis conversion to mixed alcohols, and product separation. The gas cleanup area features a catalytic fluidized-bed steam reformer to convert tars and hydrocarbons into syngas. Conversions for both the reformer and the synthesis catalysts were based on research targets expected to be achieved by 2012 through ongoing research. The mass and energy calculations were used to estimate capital and operating costs that were used in a discounted cash flow rate of return analysis for the process to calculate a minimum ethanol selling price of $0.267/L ($1.01/gal) ethanol (U.S.$2005).

  2. Final Report on Development of Thermoanaerobacterium saccharolyticum for the conversion of lignocellulose to ethanol

    SciTech Connect (OSTI)

    Herring, Christopher D.; Kenealy, William R.; Shaw, A. Joe; Raman, Babu; Tschaplinski, Timothy J.; Brown, Steven D.; Davison, Brian H.; Covalla, Sean F.; Sillers, W. Ryan; Xu, Haowen; Tsakraklides, Vasiliki; Hogsett, David A.

    2012-01-24

    This project addressed the need for economical technology for the conversion of lignocellulosic biomass to fuels, specifically the conversion of pretreated hardwood to ethanol. The technology developed is a set of strains of the bacterium Thermoanaerobacterium saccharolyticum and an associated fermentation process for pretreated hardwood. Tools for genetic engineering and analysis of the organism were developed, including a markerless mutation method, a complete genome sequence and a set of gene expression profiles that show the activity of its genes under a variety of conditions relevant to lignocellulose conversion. Improved strains were generated by selection and genetic engineering to be able to produce higher amounts of ethanol (up to 70 g/L) and to be able to better tolerate inhibitory compounds from pretreated hardwood. Analysis of these strains has generated useful insight into the genetic basis for desired properties of biofuel producing organisms. Fermentation conditions were tested and optimized to achieve ethanol production targets established in the original project proposal. The approach proposed was to add cellulase enzymes to the fermentation, a method called Simultaneous Saccharification and Fermentation (SSF). We had reason to think SSF would be an efficient approach because the optimal temperature and pH for the enzymes and bacterium are very close. Unfortunately, we discovered that commercially available cellulases are inactivated in thermophilic SSF by a combination of low redox potential and ethanol. Despite this, progress was made against the fermentation targets using bacterial cellulases. Thermoanaerobacterium saccharolyticum may still prove to be a commercially viable technology should cellulase enzyme issues be addressed. Moreover, the organism was demonstrated to produce ethanol at approximately theoretical yield from oligomeric hemicellulose extracts, an ability that may prove to be uniquely valuable in pretreatment configurations in

  3. Evolutionary Perspectives on Diversity of Lignocellulose Decay Mechanisms in Basidionycetes (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    ScienceCinema (OSTI)

    Hibbett, David [Clark University

    2013-01-15

    David Hibbett from Clark University on "Evolutionary Perspectives on Diversity of Lignocellulose Decay Mechanisms in Basidiomycetes" at the 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, California.

  4. Evolutionary Perspectives on Diversity of Lignocellulose Decay Mechanisms in Basidionycetes (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    SciTech Connect (OSTI)

    Hibbett, David [Clark University] [Clark University

    2012-03-21

    David Hibbett from Clark University on "Evolutionary Perspectives on Diversity of Lignocellulose Decay Mechanisms in Basidiomycetes" at the 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, California.

  5. Degradation of lignocellulosic biomass and its subsequent utilization for the production of liquid fuels: Subcontract progress report, 1 March 1984-28 February 1985

    SciTech Connect (OSTI)

    Cooney, C.L.; Demain, A.L.; Sinskey, A.J.; Wang, D.I.C.

    1987-07-01

    This project is a coordinated effort to develop process technology for the degradation of lignocellulosic biomass and its utilization for the production of liquid fuels. Current efforts are based on our prior success in developing a single-step microbiological process for the conversion of lignocellulose to ethanol. This process utilizes a mixed culture of Clostridium thermocellum, a thermophilic celluloytic anaerobe which degrades cellulose and hemicellulose to fermentable sugars and Clostridium thermosaccharolyticum, a thermophilic anaerobe which produces high concentrations of ethanol from both hexoses and pentoses. These studies focus on the use of C. thermocellum and its cellulases for enhanced saccharification of lignocellulose and on the direct fermentation of lignocellulose to liquid fuel. Efforts on saccharification are directed to facilitate the adoption of existing fermentation ethanol plants for cellulosic substrates and to overcome the rate limiting step of saccharification in the mixed culture. 9 refs., 9 figs., 9 tabs.

  6. Composite scintillator screen

    DOE Patents [OSTI]

    Zeman, Herbert D.

    1994-01-01

    A scintillator screen for an X-ray system includes a substrate of low-Z material and bodies of a high-Z material embedded within the substrate. By preselecting the size of the bodies embedded within the substrate, the spacial separation of the bodies and the thickness of the screen, the sensitivity of the screen to X-rays within a predetermined energy range can be predicted.

  7. Addressing the Recalcitrance of Cellulose Degradation through Cellulase Discovery, Nano-scale Elucidation of Molecular Mechanisms, and Kinetic Modeling

    SciTech Connect (OSTI)

    Walker, Larry P., Bergstrom, Gary; Corgie, Stephane; Craighead, Harold; Gibson, Donna; Wilson, David

    2011-06-13

    This research project was designed to play a vital role in the development of low cost sugars from cellulosic biomass and contributing to the national effort to displace fossil fuel usage in the USA transportation sector. The goal was to expand the portfolio of cell wall degrading enzymes through innovative research at the nano-scale level, prospecting for novel cellulases and building a kinetic framework for the development of more effective enzymatic conversion processes. More precisely, the goal was to elucidate the molecular mechanisms for some cellulases that are very familiar to members of our research team and to investigate what we hope are novel cellulases or new enzyme combinations from the world of plant pathogenic fungi and bacteria. Hydrolytic activities of various cellulases and cellulase cocktails were monitored at the nanoscale of cellulose fibrils and the microscale of pretreated cellulose particles, and we integrated this insight into a heterogeneous reaction framework. The over-riding approach for this research program was the application of innovative and cutting edge optical and high-throughput screening and analysis techniques for observing how cellulases hydrolyze real substrates.

  8. Catalytic Upgrading of Thermochemical Intermediates to Hydrocarbons: Conversion of Lignocellulosic Feedstocks to Aromatic Fuels and High Value Chemicals

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 May, 2013 Technology Area Review: Thermochemical Conversion Randy Cortright PhD Virent, Inc WBS: 3.3.1.10 This presentation does not contain any proprietary, confidential, or otherwise restricted information Goal Statement Project Goal -Develop and demonstrate integration of Virent's lignocellulosic biomass solvolysis technology with Virent's BioForming® process to generate aromatic-rich hydrocarbon products for use in either fuels or chemicals applications.  Liquefaction of Biomass and

  9. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Genes identified in this study represent novel targets for reducing cell wall recalcitrance for lignocellulosic biofuels production using plant biomass. less January 2015...

  10. Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (Phb) from a Process Relevant Lignocellulosic Derived Sugar

    SciTech Connect (OSTI)

    Wang, Wei; Mohagheghi, Ali; Mittal, Ashutosh; Pilath, Heidi; Johnson, David K.

    2015-03-22

    PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgraded to hydrocarbon fuels via commercial oligomerization technologies. In recent years a great effort has been made in bacterial production of PHB, yet the production cost of the polymer is still much higher than conventional petrochemical plastics. The high cost of PHB is because the cost of the substrates can account for as much as half of the total product cost in large scale fermentation. Thus searching for cheaper and better substrates is very necessary for PHB production. In this study, we demonstrate production of PHB by Cupriavidus necator from a process relevant lignocellulosic derived sugar stream, i.e., saccharified hydrolysate slurry from pretreated corn stover. Good cell growth was observed on slurry saccharified with advanced enzymes and 40~60% of PHB was accumulated in the cells. The mechanism of inhibition in the toxic hydrolysate generated by pretreatment and saccharification of biomass, will be discussed.

  11. Isolation of levoglucosan from lignocellulosic pyrolysis oil derived from wood or waste newsprint

    DOE Patents [OSTI]

    Moens, L.

    1995-07-11

    A method is provided for preparing high purity levoglucosan from lignocellulosic pyrolysis oils derived from wood or waste newsprint. The method includes reducing wood or newsprint to fine particle sizes, treating the particles with a hot mineral acid for a predetermined period of time, and filtering off and drying resulting solid wood or newsprint material; pyrolyzing the dried solid wood or newsprint material at temperatures between about 350 and 375 C to produce pyrolysis oils; treating the oils to liquid-liquid extraction with methyl isobutyl ketone to remove heavy tar materials from the oils, and to provide an aqueous fraction mixture of the oils containing primarily levoglucosan; treating the aqueous fraction mixtures with a basic metal salt in an amount sufficient to elevate pH values to a range of about 12 to about 12.5 and adding an amount of the salt in excess of the amount needed to obtain the pH range to remove colored materials of impurities from the oil and form a slurry, and freeze-drying the resulting slurry to produce a dry solid residue; and extracting the levoglucosan from the residue using ethyl acetate solvent to produce a purified crystalline levoglucosan. 2 figs.

  12. Isolation of levoglucosan from lignocellulosic pyrolysis oil derived from wood or waste newsprint

    DOE Patents [OSTI]

    Moens, Luc

    1995-01-01

    A method is provided for preparing high purity levoglucosan from lignocellulosic pyrolysis oils derived from wood or waste newsprint. The method includes reducing wood or newsprint to fine particle sizes, treating the particles with a hot mineral acid for a predetermined period of time, and filtering off and drying resulting solid wood or newsprint material; pyrolyzing the dried solid wood or newsprint material at temperatures between about 350.degree. and 375.degree. C. to produce pyrolysis oils; treating the oils to liquid-liquid extraction with methyl isobutyl ketone to remove heavy tar materials from the oils, and to provide an aqueous fraction mixture of the oils containing primarily levoglucosan; treating the aqueous fraction mixtures with a basic metal salt in an amount sufficient to elevate pH values to a range of about 12 to about 12.5 and adding an amount of the salt in excess of the amount needed to obtain the pH range to remove colored materials of impurities from the oil and form a slurry, and freeze-drying the resulting slurry to produce a dry solid residue; and extracting the levoglucosan from the residue using ethyl acetate solvent to produce a purified crystalline levoglucosan.

  13. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Eckard, Anahita Dehkhoda; Muthukumarappan, Kasiviswanathan; Gibbons, William

    2012-01-01

    Although lignocellulosic materials have a good potential to substitute current feedstocks used for ethanol production, conversion of these materials to fermentable sugars is still not economical through enzymatic hydrolysis. High cost of cellulase has prompted research to explore techniques that can prevent from enzyme deactivation. Colloidal proteins of casein can form monolayers on hydrophobic surfaces that alleviate the de-activation of protein of interest. Scanning electron microscope (SEM), fourier transform infrared spectroscopy (FT-IR), capillary electrophoresis (CE), and Kjeldahl and BSA protein assays were used to investigate the unknown mechanism of action of induced cellulase activity during hydrolysis of casein-treated biomass. Adsorptionmore » of casein to biomass was observed with all of the analytical techniques used and varied depending on the pretreatment techniques of biomass. FT-IR analysis of amides I and II suggested that the substructure of protein from casein or skim milk were deformed at the time of contact with biomass. With no additive, the majority of one of the cellulase mono-component, 97.1 ± 1.1, was adsorbed to CS within 24 h, this adsorption was irreversible and increased by 2% after 72 h. However, biomass treatment with skim-milk and casein reduced the adsorption to 32.9% ± 6.0 and 82.8% ± 6.0, respectively.« less

  14. Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Aston, John E.; Apel, William A.; Lee, Brady D.; Thompson, David N.; Lacey, Jeffrey A.; Newby, Deborah T.; Reed, David. W.; Thompson, Vicki S.

    2015-11-05

    Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence ofmore » 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.« less

  15. Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius

    SciTech Connect (OSTI)

    Aston, John E.; Apel, William A.; Lee, Brady D.; Thompson, David N.; Lacey, Jeffrey A.; Newby, Deborah T.; Reed, David. W.; Thompson, Vicki S.

    2015-11-05

    Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.

  16. The cost of ethanol production from lignocellulosic biomass -- A comparison of selected alternative processes. Final report

    SciTech Connect (OSTI)

    Grethlein, H.E.; Dill, T.

    1993-04-30

    The purpose of this report is to compare the cost of selected alternative processes for the conversion of lignocellulosic biomass to ethanol. In turn, this information will be used by the ARS/USDA to guide the management of research and development programs in biomass conversion. The report will identify where the cost leverages are for the selected alternatives and what performance parameters need to be achieved to improve the economics. The process alternatives considered here are not exhaustive, but are selected on the basis of having a reasonable potential in improving the economics of producing ethanol from biomass. When other alternatives come under consideration, they should be evaluated by the same methodology used in this report to give fair comparisons of opportunities. A generic plant design is developed for an annual production of 25 million gallons of anhydrous ethanol using corn stover as the model substrate at $30/dry ton. Standard chemical engineering techniques are used to give first order estimates of the capital and operating costs. Following the format of the corn to ethanol plant, there are nine sections to the plant; feed preparation, pretreatment, hydrolysis, fermentation, distillation and dehydration, stillage evaporation, storage and denaturation, utilities, and enzyme production. There are three pretreatment alternatives considered: the AFEX process, the modified AFEX process (which is abbreviated as MAFEX), and the STAKETECH process. These all use enzymatic hydrolysis and so an enzyme production section is included in the plant. The STAKETECH is the only commercially available process among the alternative processes.

  17. Tailoring Lignocelluloses for a Sustainable Energy Future (462nd Brookhaven Lecture)

    SciTech Connect (OSTI)

    Liu, Chang-Jun

    2010-10-21

    Today, the world relies on fossil fuels as a primary energy resource. This resource, however, is limited and associated with rising levels of carbon dioxide in the atmosphere. In consequence, the search for renewable biofuels has become increasingly vital. Solutions thus far have focused on first-generation biofuels, such as corn ethanol and biodiesel. But this is not enough. Chang-Jun Liu of the Biology Department discusses how he and his colleagues are studying a more abundant and environmentally friendly renewable energy source — lignocellulosic biomass — found in plant cell walls. Liu explaines, plant cell walls provide unlimited quantities of renewable biomass. However, the intertwined lignin and cellulose that make up the cell walls resist decomposition, so obtaining energy from cellulosic biomass is a challenge. Liu and his colleagues are exploring the biosynthesis and molecular regulation of plant cell walls, particularly that of the most formidable polymer — lignin. With this knowledge, they will develop novel strategies to tailor plant cell wall’s structure and composition for efficient biofuel and biomaterial production.

  18. WageWorks Screen Shot(s)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    WageWorks Screen Shot(s)

  19. Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions

    SciTech Connect (OSTI)

    Yu, Chaowei; Reddy, Amitha P.; Simmons, Christopher W.; Simmons, Blake A.; Singer, Steven W.; VanderGheynst, Jean S.

    2015-12-02

    Microbial communities enriched from diverse environments have shown considerable promise for the targeted discovery of microorganisms and enzymes for bioconversion of lignocellulose to liquid fuels. While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. The enrichments were completed in a high-solid and aerobic environment at 55 °C. Community composition was examined for each enrichment to determine when a stable community was achieved. Preservation methods included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. Revived communities were examined for their ability to decompose switchgrass under high-solid and thermophilic conditions. High-throughput 16S rRNA gene sequencing of DNA extracted from enrichment samples showed that the majority of the shift in composition of the switchgrass-degrading community occurred during the initial three 2-week enrichments. Shifts in community structure upon storage occurred in all cryopreserved samples. Storage in liquid nitrogen in the absence of cryoprotectant resulted in variable preservation of dominant microorganisms in enriched samples. Cryopreservation with either DMSO or glycerol provided consistent and equivalent preservation of dominant organisms. In conclusion, a stable switchgrass-degrading microbial community was achieved after three 2-week enrichments. Dominant microorganisms were preserved equally well with DMSO and glycerol. DMSO-preserved communities required more incubation time upon revival to achieve pre-storage activity levels during high-solid thermophilic cultivation on switchgrass. Despite shifts in the community with storage, the samples were active upon revival under

  20. Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yu, Chaowei; Reddy, Amitha P.; Simmons, Christopher W.; Simmons, Blake A.; Singer, Steven W.; VanderGheynst, Jean S.

    2015-12-02

    Microbial communities enriched from diverse environments have shown considerable promise for the targeted discovery of microorganisms and enzymes for bioconversion of lignocellulose to liquid fuels. While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. The enrichments were completed in a high-solid and aerobic environment at 55 °C. Community composition was examined for each enrichment to determine when a stable community was achieved. Preservation methodsmore » included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. Revived communities were examined for their ability to decompose switchgrass under high-solid and thermophilic conditions. High-throughput 16S rRNA gene sequencing of DNA extracted from enrichment samples showed that the majority of the shift in composition of the switchgrass-degrading community occurred during the initial three 2-week enrichments. Shifts in community structure upon storage occurred in all cryopreserved samples. Storage in liquid nitrogen in the absence of cryoprotectant resulted in variable preservation of dominant microorganisms in enriched samples. Cryopreservation with either DMSO or glycerol provided consistent and equivalent preservation of dominant organisms. In conclusion, a stable switchgrass-degrading microbial community was achieved after three 2-week enrichments. Dominant microorganisms were preserved equally well with DMSO and glycerol. DMSO-preserved communities required more incubation time upon revival to achieve pre-storage activity levels during high-solid thermophilic cultivation on switchgrass. Despite shifts in the community with storage, the samples were active upon revival

  1. Ethanol and High-Value Terpene Co-Production from Lignocellulosic Biomass of Cymbopogon flexuosus and Cymbopogon martinii

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Joyce, Blake L.; Zheljazkov, Valtcho D.; Sykes, Robert; Cantrell, Charles L.; Hamilton, Choo; Mann, David G. J.; Rodriguez, Miguel; Mielenz, Jonathan R.; Astatkie, Tess; C. Neal Stewart Jr.

    2015-10-05

    Cymbopogon flexuosus, lemongrass, and C. martinii, palmarosa, are perennial grasses grown to produce essential oils for the fragrance industry. The objectives of this study were (1) to evaluate biomass and oil yields as a function of nitrogen and sulfur fertilization, and (2) to characterize their utility for lignocellulosic ethanol compared to Panicum virgatum (switchgrass). Mean biomass yields were 12.83 Mg lemongrass ha-1 and 15.11 Mg palmarosa ha-1 during the second harvest year resulting in theoretical biofuel yields of 2541 and 2569 L ethanol ha-1 respectively compared to reported 1749–3691 L ethanol ha-1 for switchgrass. Pretreated lemongrass yielded 198 mL ethanolmore » (g biomass) -1 and pretreated palmarosa yielded 170 mL ethanol (g biomass) -1. Additionally, lemongrass yielded 85.7 kg essential oil ha-1 and palmarosa yielded 67.0 kg ha-1 with an estimated value of USD $857 and $1005 ha-1. These data suggest that dual-use crops such as lemongrass and palmarosa may increase the economic viability of lignocellulosic biofuels.« less

  2. Ethanol and High-Value Terpene Co-Production from Lignocellulosic Biomass of Cymbopogon flexuosus and Cymbopogon martinii

    SciTech Connect (OSTI)

    Joyce, Blake L.; Zheljazkov, Valtcho D.; Sykes, Robert; Cantrell, Charles L.; Hamilton, Choo; Mann, David G. J.; Rodriguez, Miguel; Mielenz, Jonathan R.; Astatkie, Tess; C. Neal Stewart Jr.

    2015-10-05

    Cymbopogon flexuosus, lemongrass, and C. martinii, palmarosa, are perennial grasses grown to produce essential oils for the fragrance industry. The objectives of this study were (1) to evaluate biomass and oil yields as a function of nitrogen and sulfur fertilization, and (2) to characterize their utility for lignocellulosic ethanol compared to Panicum virgatum (switchgrass). Mean biomass yields were 12.83 Mg lemongrass ha-1 and 15.11 Mg palmarosa ha-1 during the second harvest year resulting in theoretical biofuel yields of 2541 and 2569 L ethanol ha-1 respectively compared to reported 1749–3691 L ethanol ha-1 for switchgrass. Pretreated lemongrass yielded 198 mL ethanol (g biomass) -1 and pretreated palmarosa yielded 170 mL ethanol (g biomass) -1. Additionally, lemongrass yielded 85.7 kg essential oil ha-1 and palmarosa yielded 67.0 kg ha-1 with an estimated value of USD $857 and $1005 ha-1. These data suggest that dual-use crops such as lemongrass and palmarosa may increase the economic viability of lignocellulosic biofuels.

  3. Biological Production of a Hydrocarbon Fuel Intermediate Polyhydroxybutyrate (PHB) from a Process Relevant Lignocellulosic Derived Sugar (Poster)

    SciTech Connect (OSTI)

    Wang, W.; Mittal, A.; Mohagheghi, A.; Johnson, D. K.

    2014-04-01

    PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgraded to hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a process relevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism of inhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.

  4. Conventional Medical Screening Program

    Broader source: Energy.gov [DOE]

    Medical screening is a strategy used to identify diseases or conditions in a select population at an early stage, often before signs and symptoms develop, and to refer individuals with suspicious findings to their personal physician or a specialist for further testing, diagnosis, and treatment. The program is not intended to serve as a substitute for routine medical exams through an individual's personal physician.

  5. Degradation of lignocellulosic biomass and its subsequent utilization for the production of liquid fuels: Subcontract progress report, 1 March 1983-29 February 1984

    SciTech Connect (OSTI)

    Cooney, C.L.; Demain, A.L.; Sinskey, A.J.; Wang, D.I.C.

    1987-07-01

    This project is a coordinated effort to develop process technology for the degradation of lignocellulosic biomass and its utilization for the production of liquid fuels. Current efforts are based on our prior success in developing a single-step microbiological process for the conversion of lignocellulose to ethanol. This process utilizes a mixed culture of Clostridium thermocellum, a thermophilic cellulolytic anaerobe which degrades cellulose and hemicellulose to fermentable sugars, and Clostridium thermosaccharolyticum, a thermo anaerobe which produces high concentrations of ethanol from both hexoses and pentoses. The proposed studies will focus on the use of C. thermocellum and its cellulases for enhanced saccharification of lignocellulose and on the direct fermentation of lignocellulose to the liquid fuel, butanol. Efforts on saccharification are directed to facilitate the adoption of existing fermentation ethanol plants for cellulosic substrates and to overcome the rate limiting step of saccharification in the mixed culture. The effort on butanol will extend the concept of direct fermentation to the production of this liquid fuel. 14 refs.

  6. Degradation of lignocellulosic biomass and its subsequent utilization for the production of liquid fuels: Subcontract progress report, 1 September 1981-28 February 1982

    SciTech Connect (OSTI)

    Cooney, C.L.; Demain, A.L.; Sinskey, A.J.; Wang, D.I.C.

    1987-07-01

    This project is a coordinated effort to develop process technology for the degradation of lignocellulosic biomass and its utilization for the production of liquid fuels. Current efforts are based on our prior success in developing a single-step microbiological process for the conversion of lignocellulose to ethanol. This process utilizes a mixed culture of Clostridium thermocellum, a thermophilic cellulolytic anaerobe which degrades cellulose and hemicellulose to fermentable sugars, and Clostridium thermosaccharolyticum, a thermophilic anaerobe which produces high concentrations of ethanol from both hexoses and pentoses. The proposed studies will focus on the use of C. thermocellum and its cellulases for enhanced saccharification of lignocellulose and on the direct fermentation of lignocellulose to the liquid fuel, butanol. Efforts on saccharification are directed to facilitate the adoption of existing fermentation ethanol plants for cellulosic substrates and to overcome the rate limiting step of saccharification in the mixed culture. The effort on butanol will extend the concept of direct fermentation to the production of this liquid fuel.

  7. Degradation of lignocellulosic biomass and its subsequent utilization for the production of liquid fuels: Subcontract progress report, 1 March 1981-31 August 1981

    SciTech Connect (OSTI)

    Cooney, C.L.; Demain, A.L.; Sinskey, A.J.; Wang, D.I.C.

    1987-07-01

    This project is a coordinated effort to develop process technology for the degradation of lignocellulosic biomass and its utilization for the production of liquid fuels. Current efforts are based on our prior success in developing a single-step microbiological process for the conversion of lignocellulose to ethanol. This process utilizes a mixed culture of Clostridium thermocellum, a thermophilic cellulolytic anaerobe which degrades cellulose and hemicellulose to fermentable sugars, and C. thermosaccharolyticum, a thermophilic anaerobe which produces high concentrations of ethanol from both hexoses and pentoses. The proposed studies will focus on the use of C. therocellum and its cellulases for enhanced saccharification of lignocellulose and on the direct fermentation of lignocellulose to the liquid fuel, butanol. Efforts on saccharification are directed to facilitate the adoption of existing fermentation ethanol plants for cellulosic substrates and to overcome the rate limiting step of saccharification in the mixed culture. The effort on butanol will extend the concept of direct fermentation to the production of this fuel. 55 figs., 6 tabs.

  8. Degradation of lignocellulosic biomass and its subsequent utilization for the production of liquid fuels: Subcontract progress report, 1 March 1982-31 August 1982

    SciTech Connect (OSTI)

    Cooney, C.L.; Demain, A.L.; Sinskey, A.J.; Wang, D.I.C.

    1987-07-01

    This project is a coordinated effort to develop process technology for the degradation of lignocellulosic biomass and its utilization for the production of liquid fuels. Current efforts are based on our prior success in developing a single-step microbiological process for the conversion of lignocellulose to ethanol. This process utilizes a mixed culture of Clostridium thermocellum, a thermophilic cellulolytic anaerobe which degrades cellulose and hemicellulose to fermentable sugars, and Clostridium thermosaccharolyticum, a thermophilic anaerobic which produces high concentrations of ethanol from both hexoses and pentoses. The proposed studies will focus on the use of C. thermocellum and its cellulases for enhanced saccharification of lignocellulose and on the direct fermentation of lignocellulose to the liquid fuel, butanol. Efforts on saccharification are directed to facilitate the adoption of existing fermentation ethanol plants for cellulosic substrates and to overcome the rate limiting step of saccharification in the mixed culture. The effort on butanol will extend the concept of direct fermentation to the production of this liquid fuel.

  9. Degradation of lignocellulosic biomass and its subsequent utilization for the production of liquid fuels: Subcontract progress report, 1 September 1982-28 February 1983

    SciTech Connect (OSTI)

    Cooney, C.L.; Demain, A.L.; Sinskey, A.J.; Wang, D.I.C.

    1987-07-01

    This project is a coordinated effort to develop process technology for the degradation of lignocellulosic biomass and its utilization for the production of liquid fuels. Current efforts are based on our prior success in developing a single-step microbiological process for the conversion of lignocellulose to ethanol. This process utilizes a mixed culture of Clostridium thermocellum, a thermophilic cellulolytic anaerobe which degrades cellulose and hemicellulose to fermentable sugars, and Clostridium thermosaccharolyticum, a thermophilic anaerobe which produces high concentrations of ethanol from both hexoses and pentoses. The proposed studies will focus on the use of C. thermocellum and its cellulases for enhanced saccharification of lignocellulose and on the direct fermentation of lignocellulose to the liquid fuel, butanol. Efforts on saccharification are directed to facilitate the adoption of existing fermentation ethanol plants for cellulosic substrates and to overcome the rate limiting step of saccharification in the mixed culture. The effort on butanol will extend the concept of direct fermentation to the production of this liquid fuel.

  10. Automated macromolecular crystallization screening

    DOE Patents [OSTI]

    Segelke, Brent W.; Rupp, Bernhard; Krupka, Heike I.

    2005-03-01

    An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.

  11. GPU Computational Screening

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    GPU Computational Screening of Carbon Capture Materials J. Kim 1 , A Koniges 1 , R. Martin 1 , M. Haranczyk 1 , J. Swisher 2 , and B. Smit 1,2 1 Lawrence Berkeley National Laboratory, Berkeley, CA 94720 2 Department of Chemical Engineering, University of California, Berkeley, Berkeley, CA 94720 E-mail: jihankim@lbl.gov Abstract. In order to reduce the current costs associated with carbon capture technologies, novel materials such as zeolites and metal-organic frameworks that are based on

  12. Combined Dilute Acid and Solvent Based Pretreatment of Agricultural Wastes for Efficient Lignocellulosic Fractionation and Biofuels Production

    SciTech Connect (OSTI)

    Brodeur, G.; Ramakrishnan, S.; Wilson, C.; Telotte, J.; Collier, J.; Stickel, J.

    2013-01-01

    A true biorefinery for processing lignocellulosic biomass should achieve maximum utilization of all major constituents (cellulose, hemicellulose, & lignin) within the feedstock. In this work a combined pretreatment process of dilute acid (DA) and N-methyl morpholine N-oxide (NMMO) is described that allows for both fractionation and subsequent complete hydrolysis of the feedstocks (corn stover and sugarcane bagasse). During this multi-step processing, the dilute acid pretreatment solubilizes the majority (>90%) of the hemicellulosic fraction, while the NMMO treatment yields a cellulosic fraction that is completely digestible within 48 hours at low enzyme loadings. With both the cellulosic and hemicellulosic fractions being converted into separate, dissolved sugar fractions, the remaining portion is nearly pure lignin. When used independently, DA and NMMO pretreatments are only able to achieve ~80% and ~45% cellulosic conversion, respectively. Mass balance calculations along with experimental results are used to illustrate the feasibility of separation and recycling of NMMO.

  13. Development of a Membrane-Based Separation Process for the Continuous Enzymatic Saccharification of Lignocellulosic Biomass; NREL (National Renewable Energy Laboratory)

    SciTech Connect (OSTI)

    Adhikari, B.; Pellegrino, J.; Stickel, J.; Sievers, J.

    2014-04-29

    We are currently evaluating the feasibility of performing continuous enzymatic hydrolysis of lignocellulosic biomass to product sugars using a membrane-assisted reaction/separation process. The overarching technical goals are to continuously remove the sugars—this lowers product feedback inhibition—retain and recycle active enzyme, and continuously recover the co-product of lignin. Experimental d d d currently evaluating the feasibility of performing continuous enzymatic hydrolysis of lignocellulosic biomass to product sugars using a membrane-assisted reaction/separation process. The overarching technical goals are to continuously remove the sugars -- this lowers product feedback inhibition --retain and recycle active enzyme, and continuously recover the co-product of lignin.

  14. Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover D. Humbird, R. Davis, L. Tao, C. Kinchin, D. Hsu, and A. Aden National Renewable Energy Laboratory Golden, Colorado P. Schoen, J. Lukas, B. Olthof, M. Worley, D. Sexton, and D. Dudgeon Harris Group Inc. Seattle, Washington and Atlanta, Georgia Technical Report NREL/TP-5100-47764 May 2011 NREL is a national laboratory of the U.S. Department

  15. Downregulation of GAUT12 in Populus deltoides by RNA silencing results in reduced recalcitrance, increased growth and reduced xylan and pectin in a woody biofuel feedstock

    SciTech Connect (OSTI)

    Biswal, Ajaya K.; Hao, Zhangying; Pattathil, Sivakumar; Yang, Xiaohan; Winkeler, Kim; Collins, Cassandra; Mohanty, Sushree S.; Richardson, Elizabeth A.; Gelineo-Albersheim, Ivana; Hunt, Kimberly; Ryno, David; Sykes, Robert W.; Turner, Geoffrey B.; Ziebell, Angela; Gjersing, Erica; Lukowitz, Wolfgang; Davis, Mark F.; Decker, Stephen R.; Hahn, Michael G.; Mohnen, Debra

    2015-03-12

    The inherent recalcitrance of woody bioenergy feedstocks is a major challenge for their use as a source of second-generation biofuel. Secondary cell walls that constitute the majority of hardwood biomass are rich in cellulose, xylan, and lignin. The interactions among these polymers prevent facile accessibility and deconstruction by enzymes and chemicals. Plant biomass that can with minimal pretreatment be degraded into sugars is required to produce renewable biofuels in a cost-effective manner. The following are the results: GAUT12/IRX8 is a putative glycosyltransferase proposed to be involved in secondary cell wall glucuronoxylan and/or pectin biosynthesis based on concomitant reductions of both xylan and the pectin homogalacturonan (HG) in Arabidopsis irx8 mutants. Two GAUT12 homologs exist in Populus trichocarpa, PtGAUT12.1 and PtGAUT12.2. Knockdown expression of both genes simultaneously has been shown to reduce xylan content in Populus wood. We tested the proposition that RNA interference (RNAi) downregulation of GAUT12.1 alone would lead to increased sugar release in Populus wood, that is, reduced recalcitrance, based on the hypothesis that GAUT12 synthesizes a wall structure required for deposition of xylan and that cell walls with less xylan and/or modified cell wall architecture would have reduced recalcitrance. Using an RNAi approach, we generated 11 Populus deltoides transgenic lines with 50 to 67% reduced PdGAUT12.1 transcript expression compared to wild type (WT) and vector controls. Ten of the eleven RNAi lines yielded 4 to 8% greater glucose release upon enzymatic saccharification than the controls. The PdGAUT12.1 knockdown (PdGAUT12.1-KD) lines also displayed 12 to 52% and 12 to 44% increased plant height and radial stem diameter, respectively, compared to the controls. Knockdown of PdGAUT12.1 resulted in a 25 to 47% reduction in galacturonic acid and 17 to 30% reduction in xylose without affecting total lignin content, revealing that in Populus

  16. Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass

    SciTech Connect (OSTI)

    Young, Jenna; Chung, Daehwan; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2014-10-09

    Background: Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. Different species vary in their abilities to degrade cellulose, and the presence of CelA, a bifunctional glycoside hydrolase that contains a Family 48 and a Family 9 catalytic domain, correlates well with cellulolytic ability in members of this genus. For example, C. hydrothermalis, which does not contain a CelA homolog, or a GH48 Family or GH9 Family glycoside hydrolase, is the least cellulolytic of the Caldicellulosiruptor species so far described. C. bescii, which contains CelA and expresses it constitutively, is among the most cellulolytic. In fact, CelA is the most abundant extracellular protein produced in C. bescii. The enzyme contains two catalytic units, a Family 9A-CBM3c processive endoglucanase and a Family 48 exoglucanase, joined by two Family 3b carbohydrate-binding domains. Although there are two non-reducing end-specific Family 9 and three reducing end-specific Family 48 glycoside hydrolases (producing primarily glucose and cellobiose; and cellobiose and cellotriose, respectively) in C. bescii, CelA is the only protein that combines both enzymatic activities. Results: A deletion of the celA gene resulted in a dramatic reduction in the microorganism’s ability to grow on crystalline cellulose (Avicel) and diminished growth on lignocellulosic biomass. A comparison of the overall endoglucanase and exoglucanase activities of the mutant compared with the wild-type suggests that the loss of the endoglucanase activity provided by the GH9 family domain is perhaps compensated for by other enzymes produced by the cell. In contrast, it appears that no other enzymes in the C. bescii secretome can compensate for the loss of exoglucanase activity. The change in enzymatic activity in the celA mutant resulted in a 15-fold decrease in sugar

  17. Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Young, Jenna; Chung, Daehwan; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2014-10-09

    Background: Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. Different species vary in their abilities to degrade cellulose, and the presence of CelA, a bifunctional glycoside hydrolase that contains a Family 48 and a Family 9 catalytic domain, correlates well with cellulolytic ability in members of this genus. For example, C. hydrothermalis, which does not contain a CelA homolog, or a GH48 Family or GH9 Family glycoside hydrolase, is the least cellulolytic of the Caldicellulosiruptor species so far described. C. bescii,more » which contains CelA and expresses it constitutively, is among the most cellulolytic. In fact, CelA is the most abundant extracellular protein produced in C. bescii. The enzyme contains two catalytic units, a Family 9A-CBM3c processive endoglucanase and a Family 48 exoglucanase, joined by two Family 3b carbohydrate-binding domains. Although there are two non-reducing end-specific Family 9 and three reducing end-specific Family 48 glycoside hydrolases (producing primarily glucose and cellobiose; and cellobiose and cellotriose, respectively) in C. bescii, CelA is the only protein that combines both enzymatic activities. Results: A deletion of the celA gene resulted in a dramatic reduction in the microorganism’s ability to grow on crystalline cellulose (Avicel) and diminished growth on lignocellulosic biomass. A comparison of the overall endoglucanase and exoglucanase activities of the mutant compared with the wild-type suggests that the loss of the endoglucanase activity provided by the GH9 family domain is perhaps compensated for by other enzymes produced by the cell. In contrast, it appears that no other enzymes in the C. bescii secretome can compensate for the loss of exoglucanase activity. The change in enzymatic activity in the celA mutant resulted in a 15-fold decrease in

  18. Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

    SciTech Connect (OSTI)

    Inoue, Hiroyuki; Decker, Stephen R.; Taylor, Larry E.; Yano, Shinichi; Sawayama, Shigeki

    2014-10-09

    Background: Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide clues for maximizing the hydrolysis of target biomass. Talaromyces cellulolyticus is a promising fungus for cellulase production and efficient biomass hydrolysis. Several cellulolytic enzymes purified from T. cellulolyticus were characterized in earlier studies, but the core enzymes critical for hydrolysis of lignocellulosic biomass remain unknown. Results: Six cellulolytic enzymes critical for the hydrolysis of crystalline cellulose were purified from T. cellulolyticus culture supernatant using an enzyme assay based on synergistic hydrolysis of Avicel. The purified enzymes were identified by their substrate specificities and analyses of trypsin-digested peptide fragments and were classified into the following glycosyl hydrolase (GH) families: GH3 (β-glucosidase, Bgl3A), GH5 (endoglucanase, Cel5A), GH6 (cellobiohydrolase II, Cel6A), GH7 (cellobiohydrolase I and endoglucanase, Cel7A and Cel7B, respectively), and GH10 (xylanase, Xyl10A). Hydrolysis of dilute acid-pretreated corn stover (PCS) with mixtures of the purified enzymes showed that Cel5A, Cel7B, and Xyl10A each had synergistic effects with a mixture of Cel6A and Cel7A. Cel5A seemed to be more effective in the synergistic hydrolysis of the PCS than Cel7B. The ratio of Cel5A, Cel6A, Cel7A, and Xyl10A was statistically optimized for the hydrolysis of PCS glucan in the presence of Bgl3A. The resultant mixture achieved higher PCS glucan hydrolysis at lower enzyme loading than a culture filtrate from T. cellulolyticus or a commercial enzyme preparation, demonstrating that the five enzymes play a role as core enzymes in the hydrolysis of PCS glucan. In Conclusion: Core cellulolytic enzymes in the T. cellulolyticus cellulase system were identified to Cel5A, Cel6A, Cel7A, Xyl10A, and Bgl3A and

  19. Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Inoue, Hiroyuki; Decker, Stephen R.; Taylor, Larry E.; Yano, Shinichi; Sawayama, Shigeki

    2014-10-09

    Background: Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide clues for maximizing the hydrolysis of target biomass. Talaromyces cellulolyticus is a promising fungus for cellulase production and efficient biomass hydrolysis. Several cellulolytic enzymes purified from T. cellulolyticus were characterized in earlier studies, but the core enzymes critical for hydrolysis of lignocellulosic biomass remain unknown. Results: Six cellulolytic enzymes critical for the hydrolysis of crystalline cellulose were purified from T. cellulolyticus culture supernatant using an enzyme assay based on synergistic hydrolysismore » of Avicel. The purified enzymes were identified by their substrate specificities and analyses of trypsin-digested peptide fragments and were classified into the following glycosyl hydrolase (GH) families: GH3 (β-glucosidase, Bgl3A), GH5 (endoglucanase, Cel5A), GH6 (cellobiohydrolase II, Cel6A), GH7 (cellobiohydrolase I and endoglucanase, Cel7A and Cel7B, respectively), and GH10 (xylanase, Xyl10A). Hydrolysis of dilute acid-pretreated corn stover (PCS) with mixtures of the purified enzymes showed that Cel5A, Cel7B, and Xyl10A each had synergistic effects with a mixture of Cel6A and Cel7A. Cel5A seemed to be more effective in the synergistic hydrolysis of the PCS than Cel7B. The ratio of Cel5A, Cel6A, Cel7A, and Xyl10A was statistically optimized for the hydrolysis of PCS glucan in the presence of Bgl3A. The resultant mixture achieved higher PCS glucan hydrolysis at lower enzyme loading than a culture filtrate from T. cellulolyticus or a commercial enzyme preparation, demonstrating that the five enzymes play a role as core enzymes in the hydrolysis of PCS glucan. In Conclusion: Core cellulolytic enzymes in the T. cellulolyticus cellulase system were identified to Cel5A, Cel6A, Cel7A, Xyl10A, and Bgl3A

  20. Enhanced lipid lroduction by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Fei, Qiang; O'Brien, Marykate; Nelson, Robert; Chen, Xiaowen; Lowell, Andrew; Dowe, Nancy

    2016-06-23

    Industrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for fermentation-based bioprocesses are obtained from agricultural commodities that are used as foodstuff for human beings. Lignocellulose-derived sugars as the non-food, green, and sustainable alternative carbon sources have great potential to avoid this dilemma for producing the renewable, bio-based hydrocarbon fuel precursors, such as microbial lipid. Efficient bioconversion of lignocellulose-based sugars into lipids is one of the critical parameters for industrial application. Therefore, the fed-batch cultivation, which is a common method used in industrialmore » applications, was investigated to achieve a high cell density culture along with high lipid yield and productivity. In this study, several fed-batch strategies were explored to improve lipid production using lignocellulosic hydrolysates derived from corn stover. Compared to the batch culture giving a lipid yield of 0.19 g/g, the dissolved-oxygen-stat feeding mode increased the lipid yield to 0.23 g/g and the lipid productivity to 0.33 g/L/h. The pulse feeding mode further improved lipid productivity to 0.35 g/L/h and the yield to 0.24 g/g. However, the highest lipid yield (0.29 g/g) and productivity (0.4 g/L/h) were achieved using an automated online sugar control feeding mode, which gave a dry cell weight of 54 g/L and lipid content of 59 % (w/w). The major fatty acids of the lipid derived from lignocellulosic hydrolysates were predominately palmitic acid and oleic acid, which are similar to those of conventional oilseed plants. Our results suggest that the fed-batch feeding strategy can strongly influence the lipid production. Lastly, the online sugar control feeding mode was the most appealing strategy for high cell density, lipid yield, and lipid productivity using lignocellulosic hydrolysates

  1. Rapid characterization of lignocellulosic feedstocks for fuels and chemicals: Molecular beam mass spectrometric approach

    SciTech Connect (OSTI)

    Agblevor, F.A.; Davis, M.F. [National Renewable Energy Lab., Golden, CO (United States)

    1996-12-31

    Rapid characterization of biomass feedstocks has a pivotal role in the development of biomass energy because of the large number of samples that must be analyzed due to the diversity of biomass feedstocks and the significant differences in the chemical and physical properties of these feedstocks. Several biomass feedstocks (herbaceous, woody, and agricultural residues) were screened for the effects of storage, season of harvest, geographic location, clonal, and species variation on the pyrolysis products of the feed stocks. For herbaceous species such as sericea lespedeza, the season of harvest had a significant effect on the pyrolysis products. Effects of clonal variation on the composition of hybrid poplar feedstocks was easily discerned with the molecular beam mass spectrometric analysis. The effect of geographic location on the poplar clones pyrolysis products was minimal. However in the case of switchgrass, varietal influence on the pyrolysis products was minimal, but where the plant was grown had a strong influence on the pyrolysis products of the feedstock. Significant differences because of species variation could also be shown from the pyrolysis products of various biomass feedstocks. The influence of storage time on biomass samples stored outside in the open could also be discerned from the pyrolysis products of the feedstocks. The differences noted in the pyrolysis products of the feedstocks were noted for samples which were significantly degraded during storage either through the action of microflora or weathering.

  2. Field-to-Fuel Performance Testing of Lignocellulosic Feedstocks: An Integrated Study of the Fast Pyrolysis/Hydrotreating Pathway

    SciTech Connect (OSTI)

    Howe, Daniel T.; Westover, Tyler; Carpenter, Daniel; Santosa, Daniel M.; Emerson, Rachel; Deutch, Steve; Starace, Anne; Kutnyakov, Igor V.; Lukins, Craig D.

    2015-05-21

    Feedstock composition can affect final fuel yields and quality for the fast pyrolysis and hydrotreatment upgrading pathway. However, previous studies have focused on individual unit operations rather than the integrated system. In this study, a suite of six pure lignocellulosic feedstocks (clean pine, whole pine, tulip poplar, hybrid poplar, switchgrass, and corn stover) and two blends (equal weight percentages whole pine/tulip poplar/switchgrass and whole pine/clean pine/hybrid poplar) were prepared and characterized at Idaho National Laboratory. These blends then underwent fast pyrolysis at the National Renewable Energy Laboratory and hydrotreatment at Pacific Northwest National Laboratory. Although some feedstocks showed a high fast pyrolysis bio-oil yield such as tulip poplar at 57%, high yields in the hydrotreater were not always observed. Results showed overall fuel yields of 15% (switchgrass), 18% (corn stover), 23% (tulip poplar, Blend 1, Blend 2), 24% (whole pine, hybrid poplar) and 27% (clean pine). Simulated distillation of the upgraded oils indicated that the gasoline fraction varied from 39% (clean pine) to 51% (corn stover), while the diesel fraction ranged from 40% (corn stover) to 46% (tulip poplar). Little variation was seen in the jet fuel fraction at 11 to 12%. Hydrogen consumption during hydrotreating, a major factor in the economic feasibility of the integrated process, ranged from 0.051 g/g dry feed (tulip poplar) to 0.070 g/g dry feed (clean pine).

  3. DOD - Preliminary Screening Tool | Open Energy Information

    Open Energy Info (EERE)

    Screening Tool Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: DOD - Preliminary Screening Tool Abstract This webpage contains the DOD...

  4. Techno-economic Analysis for the Thermochemical Conversion of Lignocellulosic Biomass to Ethanol via Acetic Acid Synthesis

    SciTech Connect (OSTI)

    Zhu, Yunhua; Jones, Susanne B.

    2009-04-01

    Biomass is a renewable energy resource that can be converted into liquid fuel suitable for transportation applications. As a widely available biomass form, lignocellulosic biomass can have a major impact on domestic transportation fuel supplies and thus help meet the Energy Independence and Security Act renewable energy goals (U.S. Congress 2007). This study performs a techno-economic analysis of the thermo chemical conversion of biomass to ethanol, through methanol and acetic acid, followed by hydrogenation of acetic acid to ethanol. The conversion of syngas to methanol and methanol to acetic acid are well-proven technologies with high conversions and yields. This study was undertaken to determine if this highly selective route to ethanol could provide an already established economically attractive route to ethanol. The feedstock was assumed to be wood chips at 2000 metric ton/day (dry basis). Two types of gasification technologies were evaluated: an indirectly-heated gasifier and a directly-heated oxygen-blown gasifier. Process models were developed and a cost analysis was performed. The carbon monoxide used for acetic acid synthesis from methanol and the hydrogen used for hydrogenation were assumed to be purchased and not derived from the gasifier. Analysis results show that ethanol selling prices are estimated to be $2.79/gallon and $2.81/gallon for the indirectly-heated gasifier and the directly-heated gasifier systems, respectively (1stQ 2008$, 10% ROI). These costs are above the ethanol market price for during the same time period ($1.50 - $2.50/gal). The co-production of acetic acid greatly improves the process economics as shown in the figure below. Here, 20% of the acetic acid is diverted from ethanol production and assumed to be sold as a co-product at the prevailing market prices ($0.40 - $0.60/lb acetic acid), resulting in competitive ethanol production costs.

  5. Techno-economic Analysis for the Conversion of Lignocellulosic Biomass to Gasoline via the Methanol-to-Gasoline (MTG) Process

    SciTech Connect (OSTI)

    Jones, Susanne B.; Zhu, Yunhua

    2009-05-01

    Biomass is a renewable energy resource that can be converted into liquid fuel suitable for transportation applications. As a widely available biomass form, lignocellulosic biomass can have a major impact on domestic transportation fuel supplies and thus help meet the Energy Independence and Security Act renewable energy goals (U.S. Congress 2007). With gasification technology, biomass can be converted to gasoline via methanol synthesis and methanol-to-gasoline (MTG) technologies. Producing a gasoline product that is infrastructure ready has much potential. Although the MTG technology has been commercially demonstrated with natural gas conversion, combining MTG with biomass gasification has not been shown. Therefore, a techno-economic evaluation for a biomass MTG process based on currently available technology was developed to provide information about benefits and risks of this technology. The economic assumptions used in this report are consistent with previous U.S. Department of Energy Office of Biomass Programs techno-economic assessments. The feedstock is assumed to be wood chips at 2000 metric ton/day (dry basis). Two kinds of gasification technologies were evaluated: an indirectly-heated gasifier and a directly-heated oxygen-blown gasifier. The gasoline selling prices (2008 USD) excluding taxes were estimated to be $3.20/gallon and $3.68/gallon for indirectly-heated gasified and directly-heated. This suggests that a process based on existing technology is economic only when crude prices are above $100/bbl. However, improvements in syngas cleanup combined with consolidated gasoline synthesis can potentially reduce the capital cost. In addition, improved synthesis catalysts and reactor design may allow increased yield.

  6. Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion

    SciTech Connect (OSTI)

    Martinez, Diego; Challacombe, Jean; Morgenstern, Ingo; Hibbett, David; Schmoll, Monika; Kubicek, Christian P.; Ferreira, Patricia; Ruiz-Duenas, Francisco; Martinez, Angel T.; Kersten, Phil; Hammel, Ken; Vanden Wymelenberg, Amber; Gaskell, Jill; Lindquist, Erika; Sabat, Gregorz; Splinter Bondurant, Sandra; Larrondo, Luis F.; Canessa, Paulo; Vicuna, Rafael; Yadev, Jagjit; Doddapaneni, Harshavardhan; Subramanian, Venkataramanan; Pisabarro, Antonio; Lavin, Jose L.; Oguiza, Jose A.; Master, Emma; Henrissat, Bernard; Coutinho, Pedro M.; Harris, Paul; Magnuson, Jon K.; Baker, Scott E.; Bruno, Kenneth S.; Kenealy, William; Hoegger, Patrik; Kues, Ursula; Ramaiya, Preethi; Lucas, Susan; Salamov, Asaf; Shapiro, Harris; Tu, Hank; Chee, Christine L.; Misra, Monica; Xie, Gary; Teter, Sarah; Yaver, Debbie; James, Tim; Mokrejs, Martin; Pospisek, Martin; Grigoriev, Igor V.; Brettin, T.; Rokhsar, Daniel S.; Berka, Randy; Cullen, Dan

    2009-02-10

    Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in media containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative ?-1-4 endoglucanase were expressed at high levels relative to glucose grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also upregulated under cellulolytic culture conditions were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H2O2. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H2O2 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. In particular, comparisons between P. placenta and the closely related white-rot fungus, Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which efficient depolymerization of lignin was lost.

  7. Genome, transcriptome, and secretome analysis of wood decay fungus postia placenta supports unique mechanisms of lignocellulose conversion

    SciTech Connect (OSTI)

    Martinez, Diego; Challacombe, Jean F; Misra, Monica; Xie, Gary; Brettin, Thomas; Morgenstern, Ingo; Hibbett, David; Schmoll, Monika; Kubicek, Christian P; Ferreira, Patricia; Ruiz - Duenase, Francisco J; Martinez, Angel T; Kersten, Phil; Hammel, Kenneth E; Vanden Wymelenberg, Amber; Gaskell, Jill; Lindquist, Erika; Sabati, Grzegorz; Bondurant, Sandra S; Larrondo, Luis F; Canessa, Paulo; Vicunna, Rafael; Yadavk, Jagiit; Doddapaneni, Harshavardhan; Subramaniank, Venkataramanan; Pisabarro, Antonio G; Lavin, Jose L; Oguiza, Jose A; Master, Emma; Henrissat, Bernard; Coutinho, Pedro M; Harris, Paul; Magnuson, Jon K; Baker, Scott; Bruno, Kenneth; Kenealy, William; Hoegger, Patrik J; Kues, Ursula; Ramaiva, Preethi; Lucas, Susan; Salamov, Asaf; Shapiro, Harris; Tuh, Hank; Chee, Christine L; Teter, Sarah; Yaver, Debbie; James, Tim; Mokrejs, Martin; Pospisek, Martin; Grigoriev, Igor; Rokhsar, Dan; Berka, Randy; Cullen, Dan

    2008-01-01

    Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative {beta}-1-4 endoglucanase were expressed at high levels relative to glucose grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC{center_dot}MSIMS). Also upregulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H202. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H202 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons to the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.

  8. Final Scientific/Technical Report for DE-FG02-07ER64500 Study of Lignocellulosic Material Degradation with CARS Microscopy

    SciTech Connect (OSTI)

    Xie, Xiaoliang Sunney; Ding, Shi-You

    2013-09-30

    The program of research undertaken by our Harvard group, in collaboration with Dr. Ding at the National Renewable Energy Laboratory (NREL) in Golden, CO, seeks to introduce, validate and apply a new analytical technique to study the conversion of lignocellulosic biomass into ethanol. This conversion process has been the subject of intense interest over the past few years because of its potential to provide a clean, renewable source of energy to meet increasing global demand. During the funding period, we have clearly demonstrated visualization of lignin and cellulose using intrinsic vibrational contrast with simulated Raman scattering (SRS) microscopy, developed at Harvard. Our approach offers high spatial resolution and time resolution that is sufficient to capture the kinetics of a pre?treatment process. This is reflected by the publications listed below, as well as the use of SRS microscopy at NREL as a routine analysis tool for research on lignocellulosic biomass. In our original proposal, we envisioned moving to near?field CARS imaging in order to perform chemical mapping at the nanoscale. However, given the dramatic progress made by our group in SRS imaging, we concentrated our efforts on using multi?component SRS (lignin, cellulose, lipid, water, protein, deuterated metabolites, etc.) to quantitatively understand the spatially dispersed kinetics in a variety of plant samples under a variety of conditions. In addition, we built a next generation laser system based on fiber laser technology that allowed rugged and portable instrumentation for SRS microscopy. We also pursued new imaging approaches to improve the acquisition speed of SRS imaging of lignocellulose without sacrificing signal?to?noise ratio. This allowed us to image larger volumes of tissue with higher time resolution to get a more comprehensive picture of the heterogeneity of this chemical process from the submicron up to the centimeter scale.

  9. CLAD DEGRADATION - FEPS SCREENING ARGUMENTS

    SciTech Connect (OSTI)

    R. Schreiner

    2004-10-21

    The purpose of this report is to evaluate and document the screening of the clad degradation features, events, and processes (FEPs) with respect to modeling used to support the Total System Performance Assessment-License Application (TSPA-LA). This report also addresses the effect of certain FEPs on both the cladding and the commercial spent nuclear fuel (CSNF), DOE-owned spent nuclear fuel (DSNF), and defense high-level waste (DHLW) waste forms, as appropriate to address the effects on multiple materials and both components (FEPs 2.1.09.09.0A, 2.1.09.11.0A, 2.1.11.05.0A, 2.1.12.02.0A, and 2.1.12.03.0A). These FEPs are expected to affect the repository performance during the postclosure regulatory period of 10,000 years after permanent closure. Table 1-1 provides the list of cladding FEPs, including their screening decisions (include or exclude). The primary purpose of this report is to identify and document the analysis, screening decision, and TSPA-LA disposition (for included FEPs) or screening argument (for excluded FEPs) for these FEPs related to clad degradation. In some cases, where a FEP covers multiple technical areas and is shared with other FEP reports, this report may provide only a partial technical basis for the screening of the FEP. The full technical basis for shared FEPs is addressed collectively by the sharing FEP reports. The screening decisions and associated TSPA-LA dispositions or screening arguments from all of the FEP reports are cataloged in a project-specific FEPs database.

  10. Process Design Report for Stover Feedstock: Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover

    SciTech Connect (OSTI)

    Aden, A.; Ruth, M.; Ibsen, K.; Jechura, J.; Neeves, K.; Sheehan, J.; Wallace, B.; Montague, L.; Slayton, A.; Lukas, J.

    2002-06-01

    The U.S. Department of Energy (DOE) is promoting the development of ethanol from lignocellulosic feedstocks as an alternative to conventional petroleum-based transportation fuels. DOE funds both fundamental and applied research in this area and needs a method for predicting cost benefits of many research proposals. To that end, the National Renewable Energy Laboratory (NREL) has modeled many potential process designs and estimated the economics of each process during the last 20 years. This report is an update of the ongoing process design and economic analyses at NREL.

  11. Rare earth phosphors and phosphor screens

    DOE Patents [OSTI]

    Buchanan, Robert A.; Maple, T. Grant; Sklensky, Alden F.

    1981-01-01

    This invention relates to rare earth phosphor screens for converting image carrying incident radiation to image carrying visible or near-visible radiation and to the rare earth phosphor materials utilized in such screens. The invention further relates to methods for converting image carrying charged particles to image carrying radiation principally in the blue and near-ultraviolet region of the spectrum and to stabilized rare earth phosphors characterized by having a continuous surface layer of the phosphors of the invention. More particularly, the phosphors of the invention are oxychlorides and oxybromides of yttrium, lanthanum and gadolinium activated with trivalent cerium and the conversion screens are of the type illustratively including x-ray conversion screens, image amplifier tube screens, neutron imaging screens, cathode ray tube screens, high energy gamma ray screens, scintillation detector screens and screens for real-time translation of image carrying high energy radiation to image carrying visible or near-visible radiation.

  12. Microelectroporation device for genomic screening

    DOE Patents [OSTI]

    Perroud, Thomas D.; Renzi, Ronald F.; Negrete, Oscar; Claudnic, Mark R.

    2014-09-09

    We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

  13. Chemical compatibility screening test results

    SciTech Connect (OSTI)

    Nigrey, P.J.; Dickens, T.G.

    1997-12-01

    A program for evaluating packaging components that may be used in transporting mixed-waste forms has been developed and the first phase has been completed. This effort involved the screening of ten plastic materials in four simulant mixed-waste types. These plastics were butadiene-acrylonitrile copolymer rubber, cross-linked polyethylene (XLPE), epichlorohydrin rubber, ethylene-propylene rubber (EPDM), fluorocarbon (Viton or Kel-F), polytetrafluoroethylene, high-density polyethylene (HDPE), isobutylene-isoprene copolymer rubber (butyl), polypropylene, and styrene-butadiene rubber (SBR). The selected simulant mixed wastes were (1) an aqueous alkaline mixture of sodium nitrate and sodium nitrite; (2) a chlorinated hydrocarbon mixture; (3) a simulant liquid scintillation fluid; and (4) a mixture of ketones. The testing protocol involved exposing the respective materials to 286,000 rads of gamma radiation followed by 14-day exposures to the waste types at 60{degrees}C. The seal materials were tested using vapor transport rate (VTR) measurements while the liner materials were tested using specific gravity as a metric. For these tests, a screening criterion of 0.9 g/hr/m{sup 2} for VTR and a specific gravity change of 10% was used. Based on this work, it was concluded that while all seal materials passed exposure to the aqueous simulant mixed waste, EPDM and SBR had the lowest VTRs. In the chlorinated hydrocarbon simulant mixed waste, only Viton passed the screening tests. In both the simulant scintillation fluid mixed waste and the ketone mixture simulant mixed waste, none of the seal materials met the screening criteria. For specific gravity testing of liner materials, the data showed that while all materials with the exception of polypropylene passed the screening criteria, Kel-F, HDPE, and XLPE offered the greatest resistance to the combination of radiation and chemicals.

  14. DOE: Former Worker Medical Screening Program (FWP)

    Broader source: Energy.gov [DOE]

    FWP provides no-cost medical screenings to all former DOE Federal, contractor and subcontractor employees. The screening exams are offered by third party providers from universities, labor unions,...

  15. EJSCREEN: Environmental Justice Screening and Mapping Tool

    Broader source: Energy.gov [DOE]

    EJSCREEN is an environmental justice mapping and screening tool provided by the U.S. Environmental Protection Agency (EPA).

  16. Clad Degradation - FEPs Screening Arguments

    SciTech Connect (OSTI)

    E. Siegmann

    2004-03-17

    The purpose of this report is to document the screening of the cladding degradation features, events, and processes (FEPs) for commercial spent nuclear fuel (CSNF). This report also addresses the effect of some FEPs on both the cladding and the CSNF, DSNF, and HLW waste forms where it was considered appropriate to address the effects on both materials together. This report summarizes the work of others to screen clad degradation FEPs in a manner consistent with, and used in, the Total System Performance Assessment-License Application (TSPA-LA). This document was prepared according to ''Technical Work Plan for Waste Form Degradation Modeling, Testing, and Analyses in Support of LA'' (BSC 2004a [DIRS 167796]).

  17. HUD CHP GUIDE #2 - FEASIBILITY SCREENING FOR CHP IN MULTIFAMILY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    screening exercise and shows the screens for the feasibility screening tool, computer software prepared for HUD by the Oak Ridge National Laboratory (ORNL). chpguide2.pdf ...

  18. Federal Renewable Energy Screening Assistant

    SciTech Connect (OSTI)

    Shelpuk, B; Walker, A

    1994-10-01

    The Federal Renewable Energy Screening Assistant is a software tool to be used by energy auditors to prioritize future studies of potentially cost-effective renewable energy applications at federal facilities. This paper describes the structure and function of the tool, gives an inventory of renewable energy technologies represented in the tool, and briefly describes the algorithms used to rank opportunities by the savings-to-investment ratio.

  19. National Supplemental Screening Program | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    National Supplemental Screening Program The National Supplemental Screening Program (NSSP) offers medical screenings at no charge for former U.S. Department of Energy (DOE) site workers who may have been exposed to hazardous substances at work. For more information, see the documents below. PDF icon Retiree_Benefits_NSSPbrochure.pdf PDF icon Retiree_Benefits_newtest.pdf PDF icon Retiree_Benefits_NSSPemployees

  20. Projection screen having reduced ambient light scattering

    SciTech Connect (OSTI)

    Sweatt, William C.

    2010-05-11

    An apparatus and method for improving the contrast between incident projected light and ambient light reflected from a projection screen are described. The efficiency of the projection screen for reflection of the projected light remains high, while permitting the projection screen to be utilized in a brightly lighted room. Light power requirements from the projection system utilized may be reduced.

  1. Lignocellulosic feedstock resource assessment

    SciTech Connect (OSTI)

    Rooney, T.

    1998-09-01

    This report provides overall state and national information on the quantity, availability, and costs of current and potential feedstocks for ethanol production in the United States. It characterizes end uses and physical characteristics of feedstocks, and presents relevant information that affects the economic and technical feasibility of ethanol production from these feedstocks. The data can help researchers focus ethanol conversion research efforts on feedstocks that are compatible with the resource base.

  2. Coal storage hopper with vibrating screen agitator

    DOE Patents [OSTI]

    Daw, Charles S.; Lackey, Mack E.; Sy, Ronald L.

    1984-01-01

    The present invention is directed to a vibrating screen agitator in a coal storage hopper for assuring the uniform feed of coal having sufficient moisture content to effect agglomeration and bridging thereof in the coal hopper from the latter onto a conveyor mechanism. The vibrating screen agitator is provided by a plurality of transversely oriented and vertically spaced apart screens in the storage hopper with a plurality of vertically oriented rods attached to the screens. The rods are vibrated to effect the vibration of the screens and the breaking up of agglomerates in the coal which might impede the uniform flow of the coal from the hopper onto a conveyer.

  3. Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover

    SciTech Connect (OSTI)

    Aden, A.; Ruth, M.; Ibsen, K.; Jechura, J.; Neeves, K.; Sheehan, J.; Wallace, B.; Montague, L.; Slayton, A.; Lukas, J.

    2002-06-01

    This report is an update of NREL's ongoing process design and economic analyses of processes related to developing ethanol from lignocellulosic feedstocks. The U.S. Department of Energy (DOE) is promoting the development of ethanol from lignocellulosic feedstocks as an alternative to conventional petroleum-based transportation fuels. DOE funds both fundamental and applied research in this area and needs a method for predicting cost benefits of many research proposals. To that end, the National Renewable Energy Laboratory (NREL) has modeled many potential process designs and estimated the economics of each process during the last 20 years. This report is an update of the ongoing process design and economic analyses at NREL. We envision updating this process design report at regular intervals; the purpose being to ensure that the process design incorporates all new data from NREL research, DOE funded research and other sources, and that the equipment costs are reasonable and consistent with good engineering practice for plants of this type. For the non-research areas this means using equipment and process approaches as they are currently used in industrial applications. For the last report, published in 1999, NREL performed a complete review and update of the process design and economic model for the biomass-to-ethanol process utilizing co-current dilute acid prehydrolysis with simultaneous saccharification (enzymatic) and co-fermentation. The process design included the core technologies being researched by the DOE: prehydrolysis, simultaneous saccharification and co-fermentation, and cellulase enzyme production. In addition, all ancillary areas--feed handling, product recovery and purification, wastewater treatment (WWT), lignin combustor and boiler-turbogenerator, and utilities--were included. NREL engaged Delta-T Corporation (Delta-T) to assist in the process design evaluation, the process equipment costing, and overall plant integration. The process design and

  4. BSCL Use Plan: Solving Biomass Recalcitrance

    SciTech Connect (OSTI)

    Himmel, M.; Vinzant, T.; Bower, S.; Jechura, J.

    2005-08-01

    Technical report describing NREL's new Biomass Surface Characterization Laboratory (BSCL). The BSCL was constructed to provide the most modern commercial surface characterization equipment for studying biomass surfaces.

  5. Reverberatory screen for a radiant burner

    DOE Patents [OSTI]

    Gray, Paul E.

    1999-01-01

    The present invention relates to porous mat gas fired radiant burner panels utilizing improved reverberatory screens. The purpose of these screens is to boost the overall radiant output of the burner relative to a burner using no screen and the same fuel-air flow rates. In one embodiment, the reverberatory screen is fabricated from ceramic composite material, which can withstand higher operating temperatures than its metallic equivalent. In another embodiment the reverberatory screen is corrugated. The corrugations add stiffness which helps to resist creep and thermally induced distortions due to temperature or thermal expansion coefficient differences. As an added benefit, it has been unexpectedly discovered that the corrugations further increase the radiant efficiency of the burner. In a preferred embodiment, the reverberatory screen is both corrugated and made from ceramic composite material.

  6. Construction Worker Screening Projects | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Worker Screening Projects Construction Worker Screening Projects Sites listed below are the primary DOE sites served. Construction workers from DOE sites not listed below are covered by the Building Trades National Medical Screening Program (BTMed). Additional information regarding BTMed can be found on their website or by calling 1-800-866-9663. Alaska: Amchitka California: Lawrence Berkeley National Laboratory Lawrence Livermore National Laboratory Sandia National Laboratories (Livermore, CA)

  7. National Supplemental Screening Program | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Supplemental Screening Program National Supplemental Screening Program For more information regarding the National Supplemental Screening Program, please call toll-free at (866) 812-6703 or visit their website at http://www.orau.org/nssp. This program serves the following populations: Sites not covered by regional projects (please see Covered Sites/Populations for a complete list of regional construction worker and production workers projects); and Former workers from sites served by regional

  8. Coiled tubing cuts horizontal screen repair cost

    SciTech Connect (OSTI)

    Crow, W.; Hill, P.; Johnston, R.

    1996-01-01

    This article presents a case history of the successful workover performed by a coiled tubing unit (CTU) on Mississippi Canyon (MC) Block 109 Well A-24 in the US Gulf of Mexico to clean out sand and install new concentric screen for sand control. Workover design and operational details discussed are: Workover design -- hole-cleaning hydraulics, CT and screen predictions and comparison considerations; Workover operations -- cleanout, running packer and screens, coiled tubing (CT) weights, acid treatment and nitrogen lift and flow back.

  9. Production Worker Screening Projects | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Production Worker Screening Projects Production Worker Screening Projects Sites listed below are the primary DOE sites served. Production workers from DOE sites not listed below are covered by the National Supplemental Screening Program (NSSP). Additional information regarding NSSP can be found on their website or by calling 1-866-812-6703. California: Lawrence Berkeley National Laboratory Lawrence Livermore National Laboratory Sandia National Laboratories (Livermore, CA) Colorado: Rocky Flats

  10. Information, Planning, and Conservation (IPAC) online screening...

    Open Energy Info (EERE)

    Information, Planning, and Conservation (IPAC) online screening tool Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- OtherOther: Information,...

  11. Screening potential in high density plasmas

    SciTech Connect (OSTI)

    Amari, M.; Arranz, J. P.; Butaux, J.; Nguyen, H.

    1997-01-05

    On the basis of a two-ion center model, an accurate closed form of the screening potential is suggested for intermediate and high density plasmas.

  12. Vermont Small Hydropower Assistance Program Screening Criteria...

    Open Energy Info (EERE)

    LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: Vermont Small Hydropower Assistance Program Screening Criteria Summary and Application InstructionsPermitting...

  13. Water Project Screening Tool | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    to quickly screen sites for water-efficiency opportunities. The objective of the tool is to help federal sites identify a comprehensive suite of water efficiency projects, ...

  14. BCHP Screening Tool | Open Energy Information

    Open Energy Info (EERE)

    Screening Tool AgencyCompany Organization: Oak Ridge National Laboratory Sector: Energy Focus Area: Buildings, Industry Phase: Determine Baseline, Develop Goals Resource...

  15. Screen Electrode Materials & Cell Chemistries and Streamlining...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Screening of Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrodes Vehicle Technologies Office Merit Review 2015: Materials Benchmarking Activities for ...

  16. Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Catalytic Conversion of Sugars to Hydrocarbons

    SciTech Connect (OSTI)

    Davis, R.; Tao, L.; Scarlata, C.; Tan, E. C. D.; Ross, J.; Lukas, J.; Sexton, D.

    2015-03-01

    This report describes one potential conversion process to hydrocarbon products by way of catalytic conversion of lignocellulosic-derived hydrolysate. This model leverages expertise established over time in biomass deconstruction and process integration research at NREL, while adding in new technology areas for sugar purification and catalysis. The overarching process design converts biomass to die die diesel- and naphtha-range fuels using dilute-acid pretreatment, enzymatic saccharification, purifications, and catalytic conversion focused on deoxygenating and oligomerizing biomass hydrolysates.

  17. Screening tests report. Volume I

    SciTech Connect (OSTI)

    Not Available

    1984-07-01

    A Pressurized Fluidized Bed Combustion (PFBC) Experimental Facility has been established by UK, US and FRG Sponsors under the auspices of the International Energy Agency at Grimethorpe, South Yorkshire, England. The objective is to study combustion, sulfur removal, heat transfer, emissions, gas clean-up, corrosion and energy recovery in PFBC systems. The facility has undergone a number of modifications as a result of experience gained in a program of experimental operation with a UK datum coal and sorbent. Before making further planned modifications, and embarking on a program of experimental operation with US and FRG coal/sorbent combinations, a short series of tests was performed to establish the basic combustion parameters and to forewarn the project of any operational problem related to particular coal/sorbent combinations. This series of tests, the Screening Tests, is described in the present report. Bed material agglomerated during some of the Screening Test runs, and the operating conditions were altered from those originally planned in an attempt to minimize the occurrence. It is now believed that agglomeration resulted from changes that had been made to combustor design details and start-up procedures in an attempt to alleviate tube bank metal wastage. These factors have been subsequently corrected. The data obtained over the revized range of operating conditions included those relating to combustion and sulfur retention performance, in-bed tube bank metal wastage, gaseous and particulate emissions and the behavior of static turbine blades in a cascade. The information provided, in advance of the comprehensive series of tests with the US and FRG coal/sorbent combinations, the preliminary characterization required.

  18. Mixed Alcohol Synthesis Catalyst Screening

    SciTech Connect (OSTI)

    Gerber, Mark A.; White, James F.; Stevens, Don J.

    2007-09-03

    National Renewable Energy Laboratory (NREL) and Pacific Northwest National Laboratory (PNNL) are conducting research to investigate the feasibility of producing mixed alcohols from biomass-derived synthesis gas (syngas). PNNL is tasked with obtaining commercially available or preparing promising mixed-alcohol catalysts and screening them in a laboratory-scale reactor system. Commercially available catalysts and the most promising experimental catalysts are provided to NREL for testing using a slipstream from a pilot-scale biomass gasifier. From the standpoint of producing C2+ alcohols as the major product, it appears that the rhodium catalyst is the best choice in terms of both selectivity and space-time yield (STY). However, unless the rhodium catalyst can be improved to provide minimally acceptable STYs for commercial operation, mixed alcohol synthesis will involve significant production of other liquid coproducts. The modified Fischer-Tropsch catalyst shows the most promise for providing both an acceptable selectivity to C2+ alcohols and total liquid STY. However, further optimization of the Fischer-Tropsch catalysts to improve selectivity to higher alcohols is highly desired. Selection of a preferred catalyst will likely entail a decision on the preferred coproduct slate. No other catalysts tested appear amenable to the significant improvements needed for acceptable STYs.

  19. Feedstock Supply System Design and Economics for Conversion of Lignocellulosic Biomass to Hydrocarbon Fuels Conversion Pathway: Fast Pyrolysis and Hydrotreating Bio-Oil Pathway "The 2017 Design Case"

    SciTech Connect (OSTI)

    Kevin L. Kenney; Kara G. Cafferty; Jacob J. Jacobson; Ian J. Bonner; Garold L. Gresham; J. Richard Hess; William A. Smith; David N. Thompson; Vicki S. Thompson; Jaya Shankar Tumuluru; Neal Yancey

    2014-01-01

    The U.S. Department of Energy promotes the production of liquid fuels from lignocellulosic biomass feedstocks by funding fundamental and applied research that advances the state of technology in biomass sustainable supply, logistics, conversion, and overall system sustainability. As part of its involvement in this program, Idaho National Laboratory (INL) investigates the feedstock logistics economics and sustainability of these fuels. Between 2000 and 2012, INL quantified and the economics and sustainability of moving biomass from the field or stand to the throat of the conversion process using conventional equipment and processes. All previous work to 2012 was designed to improve the efficiency and decrease costs under conventional supply systems. The 2012 programmatic target was to demonstrate a biomass logistics cost of $55/dry Ton for woody biomass delivered to fast pyrolysis conversion facility. The goal was achieved by applying field and process demonstration unit-scale data from harvest, collection, storage, preprocessing, handling, and transportation operations into INL’s biomass logistics model.

  20. Process Design and Economics for the Conversion of Lignocellulosic Biomass to High Octane Gasoline: Thermochemical Research Pathway with Indirect Gasification and Methanol Intermediate

    SciTech Connect (OSTI)

    Tan, Eric; Talmadge, M.; Dutta, Abhijit; Hensley, Jesse; Schaidle, Josh; Biddy, Mary J.; Humbird, David; Snowden-Swan, Lesley J.; Ross, Jeff; Sexton, Danielle; Yap, Raymond; Lukas, John

    2015-03-01

    The U.S. Department of Energy (DOE) promotes research for enabling cost-competitive liquid fuels production from lignocellulosic biomass feedstocks. The research is geared to advance the state of technology (SOT) of biomass feedstock supply and logistics, conversion, and overall system sustainability. As part of their involvement in this program, the National Renewable Energy Laboratory (NREL) and the Pacific Northwest National Laboratory (PNNL) investigate the economics of conversion pathways through the development of conceptual biorefinery process models. This report describes in detail one potential conversion process for the production of high octane gasoline blendstock via indirect liquefaction (IDL). The steps involve the conversion of biomass to syngas via indirect gasification followed by gas cleanup and catalytic syngas conversion to a methanol intermediate; methanol is then further catalytically converted to high octane hydrocarbons. The conversion process model leverages technologies previously advanced by research funded by the Bioenergy Technologies Office (BETO) and demonstrated in 2012 with the production of mixed alcohols from biomass. Biomass-derived syngas cleanup via tar and hydrocarbons reforming was one of the key technology advancements as part of that research. The process described in this report evaluates a new technology area with downstream utilization of clean biomass-syngas for the production of high octane hydrocarbon products through a methanol intermediate, i.e., dehydration of methanol to dimethyl ether (DME) which subsequently undergoes homologation to high octane hydrocarbon products.

  1. The MORPHEUS II protein crystallization screen

    SciTech Connect (OSTI)

    Gorrec, Fabrice

    2015-06-27

    MORPHEUS II is a 96-condition initial crystallization screen formulated de novo. The screen incorporates reagents selected from the Protein Data Bank to yield crystals that are not observed in traditional conditions. In addition, the formulation facilitates the optimization and cryoprotection of crystals. High-quality macromolecular crystals are a prerequisite for the process of protein structure determination by X-ray diffraction. Unfortunately, the relative yield of diffraction-quality crystals from crystallization experiments is often very low. In this context, innovative crystallization screen formulations are continuously being developed. In the past, MORPHEUS, a screen in which each condition integrates a mix of additives selected from the Protein Data Bank, a cryoprotectant and a buffer system, was developed. Here, MORPHEUS II, a follow-up to the original 96-condition initial screen, is described. Reagents were selected to yield crystals when none might be observed in traditional initial screens. Besides, the screen includes heavy atoms for experimental phasing and small polyols to ensure the cryoprotection of crystals. The suitability of the resulting novel conditions is shown by the crystallization of a broad variety of protein samples and their efficiency is compared with commercially available conditions.

  2. Smart Screening System (S3) In Taconite

    SciTech Connect (OSTI)

    Daryoush Allaei

    2006-09-08

    The conventional screening machines used in processing plants have had undesirable high noise and vibration levels. They also have had unsatisfactorily low screening efficiency, high energy consumption, high maintenance cost, low productivity, and poor worker safety. These conventional vibrating machines have been used in almost every processing plant. Most of the current material separation technology uses heavy and inefficient electric motors with an unbalanced rotating mass to generate the shaking. In addition to being excessively noisy, inefficient, and high-maintenance, these vibrating machines are often the bottleneck in the entire process. Furthermore, these motors, along with the vibrating machines and supporting structure, shake other machines and structures in the vicinity. The latter increases maintenance costs while reducing worker health and safety. The conventional vibrating fine screens at taconite processing plants have had the same problems as those listed above. This has resulted in lower screening efficiency, higher energy and maintenance cost, and lower productivity and workers safety concerns. The focus of this work is on the design of a high performance screening machine suitable for taconite processing plants. SmartScreens{trademark} technology uses miniaturized motors, based on smart materials, to generate the shaking. The underlying technologies are Energy Flow Control{trademark} and Vibration Control by Confinement{trademark}. These concepts are used to direct energy flow and confine energy efficiently and effectively to the screen function. The SmartScreens{trademark} technology addresses problems related to noise and vibration, screening efficiency, productivity, and maintenance cost and worker safety. Successful development of SmartScreens{trademark} technology will bring drastic changes to the screening and physical separation industry. The final designs for key components of the SmartScreens{trademark} have been developed. The key

  3. Updating Interconnection Screens for PV System Integration

    SciTech Connect (OSTI)

    Coddington, M.; Mather, B.; Kroposki, B.; Lynn, K.; Razon, A.; Ellis, A.; Hill, R.; Key, T.; Nicole, K.; Smith, J.

    2012-02-01

    This white paper evaluates the origins and usefulness of the capacity penetration screen, offer short-term solutions which could effectively allow fast-track interconnection to many PV system applications, and considers longer-term solutions for increasing PV deployment levels in a safe and reliable manner while reducing or eliminating the emphasis on the penetration screen. Short-term and longer-term alternatives approaches are offered as examples; however, specific modifications to screening procedures should be discussed with stakeholders and must ultimately be adopted by state and federal regulatory bodies.

  4. HT Combinatorial Screening of Novel Materials for High Capacity...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen Storage HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen Storage Presentation for ...

  5. 2013 Former Worker Medical Screening Program Annual Report |...

    Energy Savers [EERE]

    3 Former Worker Medical Screening Program Annual Report 2013 Former Worker Medical Screening Program Annual Report February 2014 The 2013 Annual Report presents a detailed overview...

  6. FEMP Completes 2000th Renewable Energy Optimization Screening...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FEMP Completes 2000th Renewable Energy Optimization Screening FEMP Completes 2000th Renewable Energy Optimization Screening July 23, 2015 - 12:03pm Addthis REopt models the complex ...

  7. NETL Studies High Throughput Membrane Screening | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NETL Studies High Throughput Membrane Screening NETL Studies High Throughput Membrane Screening Membranes offer a potential low-maintenance and economical method for gas ...

  8. Host Lipid and Temperature as Important Screening Variables for...

    Office of Scientific and Technical Information (OSTI)

    Host Lipid and Temperature as Important Screening Variables for Crystallizing Integral ... Screening Variables for Crystallizing Integral Membrane Proteins in Lipidic Mesophases. ...

  9. Ecological Screening Values for Surface Water, Sediment, and...

    Office of Scientific and Technical Information (OSTI)

    Ecological Screening Values for Surface Water, Sediment, and Soil Citation Details In-Document Search Title: Ecological Screening Values for Surface Water, Sediment, and Soil ...

  10. SEP Cost Effectiveness ("Screening Tool") | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ("Screening Tool") File Screening Tool More Documents & Publications Adjustment Data Report for Fiscal Years Prior to 2008 Office of Legacy Management FY 2009 Energy...

  11. Dainippon Screen Mfg Co | Open Energy Information

    Open Energy Info (EERE)

    Mfg Co Jump to: navigation, search Name: Dainippon Screen Mfg Co Place: Kyoto, Kyoto, Japan Sector: Solar Product: Japan-based company engaged in the manufacture and sale of...

  12. Office of Worker Screening and Compensation Support

    Broader source: Energy.gov [DOE]

    The Office of Worker Screening and Compensation Programs implement the Former Worker Medical Surveillance Program and supports the Department of Labor (DOL) in the implementation of the Energy Employees Occupational Illness Compensation Program Act (EEOICPA).

  13. The electron screening puzzle and nuclear clustering

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Spitaleri, C.; Bertulani, C. A.; Fortunato, L.; Vitturi, A.

    2016-02-12

    Accurate measurements of nuclear reactions of astrophysical interest within, or close to, the Gamow peak show evidence of an unexpected effect attributed to the presence of atomic electrons in the target. The experiments need to include an effective "screening" potential to explain the enhancement of the cross sections at the lowest measurable energies. Despite various theoretical studies conducted over the past 20 years and numerous experimental measurements, a theory has not yet been found that can explain the cause of the exceedingly high values of the screening potential needed to explain the data. Furthermore, in this letter we show thatmore » instead of an atomic physics solution of the "electron screening puzzle", the reason for the large screening potential values is in fact due to clusterization effects in nuclear reactions, in particular for reaction involving light nuclei.« less

  14. Genetic Determinants for Enzymatic Digestion of Lignocellulosic Biomass Are Independent of Those for Lignin Abundance in a Maize Recombinant Inbred Population

    SciTech Connect (OSTI)

    Penning, Bryan W.; Sykes, Robert W.; Babcock, Nicholas C.; Dugard, Christopher K.; Held, Michael A.; Klimek, John F.; Shreve, Jacob T.; Fowler, Matthew; Ziebell, Angela; Davis, Mark F.; Decker, Stephen R.; Turner, Geoffrey B.; Mosier, Nathan S.; Springer, Nathan M.; Thimmapuram, Jyothi; Weil, Clifford F.; McCann, Maureen C.; Carpita, Nicholas C.

    2014-06-27

    Biotechnological approaches to reduce or modify lignin in biomass crops are predicated on the assumption that it is the principal determinant of the recalcitrance of biomass to enzymatic digestion for biofuels production. We defined quantitative trait loci (QTL) in the Intermated B73 x 3 Mo17 recombinant inbred maize (Zea mays) population using pyrolysis molecular-beam mass spectrometry to establish stem lignin content and an enzymatic hydrolysis assay to measure glucose and xylose yield. Among five multiyear QTL for lignin abundance, two for 4-vinylphenol abundance, and four for glucose and/or xylose yield, not a single QTL for aromatic abundance and sugar yield was shared. A genome-wide association study for lignin abundance and sugar yield of the 282- member maize association panel provided candidate genes in the 11 QTL of the B73 and Mo17 parents but showed that many other alleles impacting these traits exist among this broader pool of maize genetic diversity. B73 and Mo17 genotypes exhibited large differences in gene expression in developing stem tissues independent of allelic variation. Combining these complementary genetic approaches provides a narrowed list of candidate genes. A cluster of SCARECROW-LIKE9 and SCARECROW-LIKE14 transcription factor genes provides exceptionally strong candidate genes emerging from the genome-wide association study. In addition to these and genes associated with cell wall metabolism, candidates include several other transcription factors associated with vascularization and fiber formation and components of cellular signaling pathways. Finally, these results provide new insights and strategies beyond the modification of lignin to enhance yields of biofuels from genetically modified biomass.

  15. Genetic Determinants for Enzymatic Digestion of Lignocellulosic Biomass Are Independent of Those for Lignin Abundance in a Maize Recombinant Inbred Population

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Penning, Bryan W.; Sykes, Robert W.; Babcock, Nicholas C.; Dugard, Christopher K.; Held, Michael A.; Klimek, John F.; Shreve, Jacob T.; Fowler, Matthew; Ziebell, Angela; Davis, Mark F.; et al

    2014-06-27

    Biotechnological approaches to reduce or modify lignin in biomass crops are predicated on the assumption that it is the principal determinant of the recalcitrance of biomass to enzymatic digestion for biofuels production. We defined quantitative trait loci (QTL) in the Intermated B73 x 3 Mo17 recombinant inbred maize (Zea mays) population using pyrolysis molecular-beam mass spectrometry to establish stem lignin content and an enzymatic hydrolysis assay to measure glucose and xylose yield. Among five multiyear QTL for lignin abundance, two for 4-vinylphenol abundance, and four for glucose and/or xylose yield, not a single QTL for aromatic abundance and sugar yieldmore » was shared. A genome-wide association study for lignin abundance and sugar yield of the 282- member maize association panel provided candidate genes in the 11 QTL of the B73 and Mo17 parents but showed that many other alleles impacting these traits exist among this broader pool of maize genetic diversity. B73 and Mo17 genotypes exhibited large differences in gene expression in developing stem tissues independent of allelic variation. Combining these complementary genetic approaches provides a narrowed list of candidate genes. A cluster of SCARECROW-LIKE9 and SCARECROW-LIKE14 transcription factor genes provides exceptionally strong candidate genes emerging from the genome-wide association study. In addition to these and genes associated with cell wall metabolism, candidates include several other transcription factors associated with vascularization and fiber formation and components of cellular signaling pathways. Finally, these results provide new insights and strategies beyond the modification of lignin to enhance yields of biofuels from genetically modified biomass.« less

  16. Minimum Day Time Load Calculation and Screening

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Minimum Daytime Load Calculation and Screening Page 1 of 30 Kristen Ardani, Dora Nakfuji, Anthony Hong, and Babak Enayati Page 1 of 30 [Speaker: Kristen Ardani] Cover Slide: Thank you everyone for joining us today for our DG interconnection collaborative informational webinar. Today we are going to talk about minimum day time load calculation and screening procedures and their role in the distributed PV interconnection process. We're going to hear from Babak Enayati of the Massachusetts

  17. Protein Dynamics Hit the Big Screen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Dynamics Hit the Big Screen Protein Dynamics Hit the Big Screen Now playing at a supercomputer near you: proteins in action June 29, 2005 Contact: Dan Krotz, dakrotz@lbl.gov 06tyrosinekinasechanging.jpg This simulation of a tyrosine kinase reveals how the protein changes shape. Scientists from Berkeley Lab and UC Berkeley are using one the world's most powerful computers to simulate how protein molecules move, rotate, and fold as they carry out life's most fundamental tasks.Although they

  18. Screening Analysis : Volume 1, Description and Conclusions.

    SciTech Connect (OSTI)

    Bonneville Power Administration; Corps of Engineers; Bureau of Reclamation

    1992-08-01

    The SOR consists of three analytical phases leading to a Draft EIS. The first phase Pilot Analysis, was performed for the purpose of testing the decision analysis methodology being used in the SOR. The Pilot Analysis is described later in this chapter. The second phase, Screening Analysis, examines all possible operating alternatives using a simplified analytical approach. It is described in detail in this and the next chapter. This document also presents the results of screening. The final phase, Full-Scale Analysis, will be documented in the Draft EIS and is intended to evaluate comprehensively the few, best alternatives arising from the screening analysis. The purpose of screening is to analyze a wide variety of differing ways of operating the Columbia River system to test the reaction of the system to change. The many alternatives considered reflect the range of needs and requirements of the various river users and interests in the Columbia River Basin. While some of the alternatives might be viewed as extreme, the information gained from the analysis is useful in highlighting issues and conflicts in meeting operating objectives. Screening is also intended to develop a broad technical basis for evaluation including regional experts and to begin developing an evaluation capability for each river use that will support full-scale analysis. Finally, screening provides a logical method for examining all possible options and reaching a decision on a few alternatives worthy of full-scale analysis. An organizational structure was developed and staffed to manage and execute the SOR, specifically during the screening phase and the upcoming full-scale analysis phase. The organization involves ten technical work groups, each representing a particular river use. Several other groups exist to oversee or support the efforts of the work groups.

  19. Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Contract No. DE-AC36-08GO28308 Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons R. Davis, L. Tao, E.C.D. Tan, M.J. Biddy, G.T. Beckham, and C. Scarlata National Renewable Energy Laboratory J. Jacobson and K. Cafferty Idaho National Laboratory J. Ross, J. Lukas, D. Knorr, and P. Schoen Harris Group Inc. Technical Report NREL/TP-5100-60223

  20. High-throughput screening and device for photocatalysts

    DOE Patents [OSTI]

    Lewis, Nathan S.; Katz, Jordan; Gingrich, Todd

    2015-09-08

    The disclosure relates to compositions, devices and methods for screening of photocatalysts for water-splitting.

  1. Low background screening capability in the UK

    SciTech Connect (OSTI)

    Ghag, Chamkaur

    2015-08-17

    Low background rare event searches in underground laboratories seeking observation of direct dark matter interactions or neutrino-less double beta decay have the potential to profoundly advance our understanding of the physical universe. Successful results from these experiments depend critically on construction from extremely radiologically clean materials and accurate knowledge of subsequent low levels of expected background. The experiments must conduct comprehensive screening campaigns to reduce radioactivity from detector components, and these measurements also inform detailed characterisation and quantification of background sources and their impact, necessary to assign statistical significance to any potential discovery. To provide requisite sensitivity for material screening and characterisation in the UK to support our rare event search activities, we have re-developed our infrastructure to add ultra-low background capability across a range of complementary techniques that collectively allow complete radioactivity measurements. Ultra-low background HPGe and BEGe detectors have been installed at the Boulby Underground Laboratory, itself undergoing substantial facility re-furbishment, to provide high sensitivity gamma spectroscopy in particular for measuring the uranium and thorium decay series products. Dedicated low-activity mass spectrometry instrumentation has been developed at UCL for part per trillion level contaminant identification to complement underground screening with direct U and Th measurements, and meet throughput demands. Finally, radon emanation screening at UCL measures radon background inaccessible to gamma or mass spectrometry techniques. With this new capability the UK is delivering half of the radioactivity screening for the LZ dark matter search experiment.

  2. HUD CHP GUIDE #2- FEASIBILITY SCREENING FOR CHP IN MULTIFAMILY HOUSING, May 2009

    Broader source: Energy.gov [DOE]

    Guide shows the screens for the feasibility screening tool, computer software prepared for HUD by ORNL

  3. CHEMICAL SENSOR AND FIELD SCREENING TECHNOLOGY DEVELOPMENT: FUELS IN SOILS FIELD SCREENING METHOD VALIDATION

    SciTech Connect (OSTI)

    Susan S. Sorini; John F. Schabron

    1997-04-01

    A new screening method for fuel contamination in soils was recently developed as American Society for Testing and Materials (ASTM) Method D-583 1-95, Standard Test Method for Screening Fuels in Soils. This method uses low-toxicity chemicals and can be used to screen organic-rich soils. In addition, it is fast, easy, and inexpensive to perform. The screening method calls for extracting a sample of soil with isopropyl alcohol following treatment with calcium oxide. The resulting extract is filtered, and the ultraviolet absorbance of the extract is measured at 254 nm. Depending on the available information concerning the contaminant fuel type and availability of the contaminant fuel for calibration, the method can be used to determine the approximate concentration of fuel contamination, an estimated value of fuel contamination, or an indication of the presence or absence of fuel contamination. Fuels containing aromatic compounds, such as diesel fuel and gasoline, as well as other aromatic-containing hydrocarbon materials, such as motor oil, crude oil, and coal oil, can be determined. The screening method for fuels in soils was evaluated by conducting a collaborative study on the method and by using the method to screen soil samples at an actual field site. In the collaborative study, a sand and an organic soil spiked with various concentrations of diesel fuel were tested. Data from the collaborative study were used to determine the reproducibility (between participants) and repeatability (within participant) precision of the method for screening the test materials. The collaborative study data also provide information on the performance of portable field equipment versus laboratory equipment for performing the screening method and a comparison of diesel concentration values determined using the screening method versus a laboratory method. Data generated using the method to screen soil samples in the field provide information on the performance of the method in

  4. Screening the Hanford tanks for trapped gas

    SciTech Connect (OSTI)

    Whitney, P.

    1995-10-01

    The Hanford Site is home to 177 large, underground nuclear waste storage tanks. Hydrogen gas is generated within the waste in these tanks. This document presents the results of a screening of Hanford`s nuclear waste storage tanks for the presence of gas trapped in the waste. The method used for the screening is to look for an inverse correlation between waste level measurements and ambient atmospheric pressure. If the waste level in a tank decreases with an increase in ambient atmospheric pressure, then the compressibility may be attributed to gas trapped within the waste. In this report, this methodology is not used to estimate the volume of gas trapped in the waste. The waste level measurements used in this study were made primarily to monitor the tanks for leaks and intrusions. Four measurement devices are widely used in these tanks. Three of these measure the level of the waste surface. The remaining device measures from within a well embedded in the waste, thereby monitoring the liquid level even if the liquid level is below a dry waste crust. In the past, a steady rise in waste level has been taken as an indicator of trapped gas. This indicator is not part of the screening calculation described in this report; however, a possible explanation for the rise is given by the mathematical relation between atmospheric pressure and waste level used to support the screening calculation. The screening was applied to data from each measurement device in each tank. If any of these data for a single tank indicated trapped gas, that tank was flagged by this screening process. A total of 58 of the 177 Hanford tanks were flagged as containing trapped gas, including 21 of the 25 tanks currently on the flammable gas watch list.

  5. Feedstock Supply System Design and Economics for Conversion of Lignocellulosic Biomass to Hydrocarbon Fuels: Conversion Pathway: Biological Conversion of Sugars to Hydrocarbons The 2017 Design Case

    SciTech Connect (OSTI)

    Kevin Kenney; Kara G. Cafferty; Jacob J. Jacobson; Ian J Bonner; Garold L. Gresham; William A. Smith; David N. Thompson; Vicki S. Thompson; Jaya Shankar Tumuluru; Neal Yancey

    2013-09-01

    The U.S. Department of Energy promotes the production of a range of liquid fuels and fuel blendstocks from lignocellulosic biomass feedstocks by funding fundamental and applied research that advances the state of technology in biomass collection, conversion, and sustainability. As part of its involvement in this program, the Idaho National Laboratory (INL) investigates the feedstock logistics economics and sustainability of these fuels. Between 2000 and 2012, INL conducted a campaign to quantify the economics and sustainability of moving biomass from standing in the field or stand to the throat of the biomass conversion process. The goal of this program was to establish the current costs based on conventional equipment and processes, design improvements to the current system, and to mark annual improvements based on higher efficiencies or better designs. The 2012 programmatic target was to demonstrate a delivered biomass logistics cost of $35/dry ton. This goal was successfully achieved in 2012 by implementing field and process demonstration unit-scale data from harvest, collection, storage, preprocessing, handling, and transportation operations into INL’s biomass logistics model. Looking forward to 2017, the programmatic target is to supply biomass to the conversion facilities at a total cost of $80/dry ton and on specification with in-feed requirements. The goal of the 2017 Design Case is to enable expansion of biofuels production beyond highly productive resource areas by breaking the reliance of cost-competitive biofuel production on a single, abundant, low-cost feedstock. If this goal is not achieved, biofuel plants are destined to be small and/or clustered in select regions of the country that have a lock on low-cost feedstock. To put the 2017 cost target into perspective of past accomplishments of the cellulosic ethanol pathway, the $80 target encompasses total delivered feedstock cost, including both grower payment and logistics costs, while meeting all

  6. Screening and Evaluation Tool (SET) Users Guide

    SciTech Connect (OSTI)

    Layne Pincock

    2014-10-01

    This document is the users guide to using the Screening and Evaluation Tool (SET). SET is a tool for comparing multiple fuel cycle options against a common set of criteria and metrics. It does this using standard multi-attribute utility decision analysis methods.

  7. Laboratory to change vehicle traffic-screening regimen at vehicle...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Changes to vehicle traffic-screening Laboratory to change vehicle traffic-screening regimen at vehicle inspection station Lanes two through five will be open 24 hours a day and...

  8. Renewable Energy Screening for ESPCs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Renewable Energy Screening for ESPCs Renewable Energy Screening for ESPCs Document outlines the process and data needed for agencies to request screening of potential renewable energy projects from U.S. Department of Energy national laboratories for energy savings performance contracts (ESPCs). Download the renewable energy screening for ESPCs document. (246.16 KB) More Documents & Publications DOE ESPC Life of Contract Plan Template FEMP ESPC Project Development Resource Guide USCG

  9. Random Selection for Drug Screening (Technical Report) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Subject: 99 GENERAL AND MISCELLANEOUSMATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; PROBABILITY; SAMPLING; DRUG ABUSE; DRUGS; CRIME DETECTION drug screenings, random selection ...

  10. EJSCREEN: Environmental Justice Screening and Mapping Tool (EPA)

    Broader source: Energy.gov [DOE]

    EJSCREEN is an environmental justice mapping and screening tool provided by the U.S. Environmental Protection Agency (EPA).

  11. Former Worker Medical Screening Program | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Former Worker Medical Screening Program Former Worker Medical Screening Program The Former Worker Medical Screening Program (FWP) provides ongoing medical screening examinations, at no cost, to all former DOE Federal, contractor, and subcontractor workers who may be at risk for occupational diseases. The FWP is supported by the U.S. Department of Energy's (DOE) Office of Environment, Health, Safety and Security (EHSS) and reflects our commitment to the health and safety of all DOE workers - past

  12. Minimum Day Time Load Calculation and Screening

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Minimum Day Time Load Calculation and Screening" Dora Nakafuji and Anthony Hong, Hawaiian Electric Co. Babak Enayati, DG Techincal Standards Review Group April 30, 2014 2 Speakers Babak Enayati Chair of Massachusetts DG Technical Standards Review Group Dora Nakafuji Director of Renewable Energy Planning Hawaiian Electric Company (HECO) Kristen Ardani Solar Analyst, (today's moderator) NREL Anthony Hong Director of Distribution Planning Hawaiian Electric Company (HECO) Standardization of

  13. 15.12.04 Screening - JCAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How can theory help with rapid screening of promising photocatalysts in solvent? JCAP scientists are developing novel theoretical methods to predict the effect that solvents have on material properties - and consequently the photoelectrochemical performance - inside solar-fuels generators. Inside a working integrated device that uses solar energy to convert carbon dioxide and water into fuels, chemical reactions occur on solid-liquid interfaces, where the solid is a photocatalyst and the liquid

  14. 15.12.04 RH Screening - JCAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How can theory help with rapid screening of promising photocatalysts in solvent? JCAP scientists are developing novel theoretical methods to predict the effect that solvents have on material properties - and consequently the photoelectrochemical performance - inside solar-fuels generators. Inside a working integrated device that uses solar energy to convert carbon dioxide and water into fuels, chemical reactions occur on solid-liquid interfaces, where the solid is a photocatalyst and the liquid

  15. Minimum Day Time Load Calculation and Screening

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Distributed Generation Interconnection Collaborative (DGIC) "Minimum Day Time Load Calculation and Screening" Dora Nakafuji and Anthony Hong, Hawaiian Electric Co. Babak Enayati, DG Techincal Standards Review Group April 30, 2014 2 Speakers Babak Enayati Chair of Massachusetts DG Technical Standards Review Group Dora Nakafuji Director of Renewable Energy Planning Hawaiian Electric Company (HECO) Kristen Ardani Solar Analyst, (today's moderator) NREL Anthony Hong Director of

  16. Thermophilic lignocellulose deconstruction (Journal Article)...

    Office of Scientific and Technical Information (OSTI)

    North Carolina State University ORNL University of Georgia, Athens, GA Publication Date: 2013-01-01 OSTI Identifier: 1116489 DOE Contract Number: DE-AC05-00OR22725 Resource Type: ...

  17. Screening technology reduces ash in spiral circuits

    SciTech Connect (OSTI)

    Brodzik, P.

    2007-05-15

    In 2006, the James River Coal Co. selected the Stack Sizer to remove the minus 100 mesh high ash clay fraction from the clean coal spiral product circuits at the McCoy-Elkhorn Bevins Branch prep plant and at the Blue Diamond Leatherwood prep plant in Kentucky. The Stack Sizer is a multi-deck, high-frequency vibrating screen capable of separations as fine as 75 microns when fitted with Derrick Corp.'s patented high open area urethane screen panels. Full-scale lab tests and more than 10 months of continuous production have confirmed that the Stack Sizer fitted with Derrick 100 micron urethane screen panels consistently produces a clean coal fraction that ranges from 8 to 10% ash. Currently, each five-deck Stack Sizer operating at the Bevins Branch and Leatherwood prep plants is producing approximately 33 tons per hour of clean coal containing about 9% ash. This represents a clean coal yield of about 75% and an ash reduction of about 11% from the feed slurry. 3 figs. 2 tabs.

  18. Updating Technical Screens for PV Interconnection: Preprint

    SciTech Connect (OSTI)

    Coddington, M.; Ellis, A.; Lynn, K.; Razon, A.; Key, T.; Kroposki, B.; Mather, B.; Hill, R.; Nicole, K.; Smith, J.

    2012-08-01

    Solar photovoltaics (PV) is the dominant type of distributed generation (DG) technology interconnected to electric distribution systems in the United States, and deployment of PV systems continues to increase rapidly. Considering the rapid growth and widespread deployment of PV systems in United States electric distribution grids, it is important that interconnection procedures be as streamlined as possible to avoid unnecessary interconnection studies, costs, and delays. Because many PV interconnection applications involve high penetration scenarios, the process needs to allow for a sufficiently rigorous technical evaluation to identify and address possible system impacts. Existing interconnection procedures are designed to balance the need for efficiency and technical rigor for all DG. However, there is an implicit expectation that those procedures will be updated over time in order to remain relevant with respect to evolving standards, technology, and practical experience. Modifications to interconnection screens and procedures must focus on maintaining or improving safety and reliability, as well as accurately allocating costs and improving expediency of the interconnection process. This paper evaluates the origins and usefulness of the capacity penetration screen, offers potential short-term solutions which could effectively allow fast-track interconnection to many PV system applications, and considers longer-term solutions for increasing PV deployment levels in a safe and reliable manner while reducing or eliminating the emphasis on the penetration screen.

  19. Medical Screening Protocol for the Former Worker Medical Screening Program U.S. Department of Energy, October 7, 2015

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7/15 1 MEDICAL SCREENING PROTOCOL FOR THE FORMER WORKER MEDICAL SCREENING PROGRAM U.S. DEPARTMENT OF ENERGY General Principles: 1) The purpose of the medical evaluation component of the U.S. Department of Energy (DOE) Former Worker Medical Screening Program (FWP) is to provide interested former workers with targeted testing to screen for selected adverse health effects potentially related to their work in DOE operations. The program does not test for all potentially work-related conditions; for

  20. Apparatus for combinatorial screening of electrochemical materials

    DOE Patents [OSTI]

    A high throughput combinatorial screening method and apparatus for the evaluation of electrochemical materials using a single voltage source is disclosed wherein temperature changes arising from the application of an electrical load to a cell array are used to evaluate the relative electrochemical efficiency of the materials comprising the array. The apparatus may include an array of electrochemical cells that are connected to each other in parallel or in series, an electronic load for applying a voltage or current to the electrochemical cells , and a device , external to the cells, for monitoring the relative temperature of each cell when the load is applied.

    2009-12-15

    A high throughput combinatorial screening method and apparatus for the evaluation of electrochemical materials using a single voltage source (2) is disclosed wherein temperature changes arising from the application of an electrical load to a cell array (1) are used to evaluate the relative electrochemical efficiency of the materials comprising the array. The apparatus may include an array of electrochemical cells (1) that are connected to each other in parallel or in series, an electronic load (2) for applying a voltage or current to the electrochemical cells (1), and a device (3), external to the cells, for monitoring the relative temperature of each cell when the load is applied.

  1. Technologic improvements in screen-film mammography

    SciTech Connect (OSTI)

    Haus, A.G. )

    1990-03-01

    During the past 20 years, many significant technologic improvements in mammographic x-ray equipment and screen-film-processing systems have occurred. Today it is possible to obtain mammograms with higher image quality at a significantly lower radiation dose, compared with mammograms dating back about 20 years. In this review article, clinical image comparisons and technical information--including x-ray spectra, limiting geometric resolution, sensitometric characteristic curves, modulation transfer function, and noise power spectra--are used to demonstrate technologic improvements in mammographic image quality.48 references.

  2. Screening analysis of solar thermochemical hydrogen concepts.

    SciTech Connect (OSTI)

    Diver, Richard B., Jr.; Kolb, Gregory J.

    2008-03-01

    A screening analysis was performed to identify concentrating solar power (CSP) concepts that produce hydrogen with the highest efficiency. Several CSP concepts were identified that have the potential to be much more efficient than today's low-temperature electrolysis technology. They combine a central receiver or dish with either a thermochemical cycle or high-temperature electrolyzer that operate at temperatures >600 C. The solar-to-hydrogen efficiencies of the best central receiver concepts exceed 20%, significantly better than the 14% value predicted for low-temperature electrolysis.

  3. Former worekrs' notification adn medical screening Hanford

    SciTech Connect (OSTI)

    Patricia Quinn

    2005-11-30

    CPWR is carrying out a screening program for former Hanford construction workers. This program includes continuing screening and re-screening services for the former worker population. The Program contains the following general components: Start-up planning/needs assessment: A modified exposure assessment will be conducted to identify high-risk buildings or areas, primary exposures, and worker populations at risk. Outreach: CPWR, as the research arm of the Building and Construction Trades Department, AFL-CIO, has direct access to workers. CPWR will rely on direct mailings to lists of former workers, and work through and rely on existing organizations (unions, union pension funds, employers, DOE site administrators, etc.) to reach former workers and "get the word out." CPWR will establish/maintain an outreach office at each site listed above. This office will serve as the face of the Program to workers and their communities. Communications and intake center: CPWR has two established toll-free phone numbers (1-800-866-9663 and 1-888-464-0009). There is also a dedicated website for the program (btmed.org). Workers can register with the Program by mail, telephone, or on-line. Work history: A standardized, structured work history is administered with modules that accommodate unique exposure scenarios for different occupations and different DOE sites. A work history interview is administered by a trained program interviewer. The work histories are used to determine whether a participant is eligible for the medical examination and to interpret the findings from the medical examination. Medical evaluation: The Program contracts with local medical providers qualified to deliver occupational medical screening services. All providers are credentialed. The Program contracts with a certified national laboratory and with NIOSH certified B-readers to review x-rays. Based on the work history, the participant is referred to a credentialed medical provider who is located close to

  4. Evaluation of radiographers’ mammography screen-reading accuracy in Australia

    SciTech Connect (OSTI)

    Debono, Josephine C; Poulos, Ann E; Houssami, Nehmat; Turner, Robin M; Boyages, John

    2015-03-15

    This study aimed to evaluate the accuracy of radiographers’ screen-reading mammograms. Currently, radiologist workforce shortages may be compromising the BreastScreen Australia screening program goal to detect early breast cancer. The solution to a similar problem in the United Kingdom has successfully encouraged radiographers to take on the role as one of two screen-readers. Prior to consideration of this strategy in Australia, educational and experiential differences between radiographers in the United Kingdom and Australia emphasise the need for an investigation of Australian radiographers’ screen-reading accuracy. Ten radiographers employed by the Westmead Breast Cancer Institute with a range of radiographic (median = 28 years), mammographic (median = 13 years) and BreastScreen (median = 8 years) experience were recruited to blindly and independently screen-read an image test set of 500 mammograms, without formal training. The radiographers indicated the presence of an abnormality using BI-RADS®. Accuracy was determined by comparison with the gold standard of known outcomes of pathology results, interval matching and client 6-year follow-up. Individual sensitivity and specificity levels ranged between 76.0% and 92.0%, and 74.8% and 96.2% respectively. Pooled screen-reader accuracy across the radiographers estimated sensitivity as 82.2% and specificity as 89.5%. Areas under the reading operating characteristic curve ranged between 0.842 and 0.923. This sample of radiographers in an Australian setting have adequate accuracy levels when screen-reading mammograms. It is expected that with formal screen-reading training, accuracy levels will improve, and with support, radiographers have the potential to be one of the two screen-readers in the BreastScreen Australia program, contributing to timeliness and improved program outcomes.

  5. Impact of Cardiovascular Counseling and Screening in Hodgkin Lymphoma Survivors

    SciTech Connect (OSTI)

    Daniëls, Laurien A.; Krol, Stijn D.G.; Graaf, Michiel A. de; Scholte, Arthur J.H.A.; Veer, Mars B. van 't; Putter, Hein; Roos, Albert de; Schalij, Martin J.; Poll-Franse, Lonneke V. van de; Creutzberg, Carien L.

    2014-09-01

    Purpose: Cardiovascular disease (CVD) is the most common nonmalignant cause of death in Hodgkin lymphoma (HL) survivors, especially after mediastinal irradiation. The role of screening for CVD in HL survivors is unclear, but confrontation with risks of CVD may have a negative influence on health-related quality of life (HRQL). As part of a phase 2 screening study using computed tomography angiography (CTA) among HL survivors, an HRQL analysis was done to evaluate the emotional and practical burden and perceived benefits of screening and the effect of CVD-specific counseling on patient satisfaction. Methods and Materials: Patients who participated in the screening study also took part in the HRQL study. The impact of undergoing screening was evaluated with a 9-item questionnaire, and impact on HRQL with the European Organization for Research and Treatment of Cancer (EORTC) Quality of Life Core Questionnaire C30, version 3.0. The effect of counseling of CVD on perceived provision of information was evaluated with EORTC INFO-25. All questionnaires were completed at baseline and after screening. Results: Baseline questionnaires were received from 48 participants, and 43 completed questionnaires after screening. Mean age was 47 years, and mean time since diagnosis was 21 years. Of the total, 93% of subjects were content with participating, and 80% did not find the emphasis placed on late effects burdensome, although screening did have a small impact on social functioning and global quality of life. Perceived information on disease, medical tests, and treatment increased significantly after screening (P<.01). Differences were clinically relevant. There were no differences in perceived information between patients with and without screen-detected CVD. Conclusions: Screening was evaluated favorably, whether CTA showed abnormalities or not. Extensive counseling resulted in substantially increased provision of information and improved information satisfaction. Screening by

  6. Efficient Theoretical Screening of Solid Sorbents for CO2 Capture

    Office of Scientific and Technical Information (OSTI)

    Applications* (Journal Article) | SciTech Connect Journal Article: Efficient Theoretical Screening of Solid Sorbents for CO2 Capture Applications* Citation Details In-Document Search Title: Efficient Theoretical Screening of Solid Sorbents for CO2 Capture Applications* By combining thermodynamic database mining with first principles density functional theory and phonon lattice dynamics calculations, a theoretical screening methodology to identify the most promising CO2 sorbent candidates

  7. HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Storage | Department of Energy HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen Storage HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen Storage Presentation for the high temperature combinatorial screening for high capacity hydrogen storage meeting ht_ucf_raissi.pdf (999.19 KB) More Documents & Publications DetecTape - A Localized Visual Detector for Hydrogen Leaks DetecTape - A Localized Visual Detector for Hydrogen Leaks Webinar

  8. Former Worker Medical Screening Program Brochure | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Medical Screening Program Brochure Former Worker Medical Screening Program Brochure December 2014 The FWP brochure provides important information to inform former and current DOE Federal, contractor, and subcontractor employees about the benefits and services offered under the DOE Former Worker Medical Screening Program. Some of the topics described in the brochure include: a description of the program, how it is implemented, who is eligible to participate, what tests are offered, where exams

  9. Outreach (Former Worker Medical Screening Program (FWP) | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Outreach (Former Worker Medical Screening Program (FWP) Outreach (Former Worker Medical Screening Program (FWP) Outreach: Identify and Notify Eligible DOE Workers About FWP Medical Screening Services All former DOE Federal, contractor, and subcontractor employees from all facilities are eligible to participate in the program. Although the historical best estimate for the population of former workers who are entitled to receive medical evaluations under the FWP is upwards of 600,000

  10. 2014 Former Worker Medical Screening Program Annual Report | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Former Worker Medical Screening Program Annual Report 2014 Former Worker Medical Screening Program Annual Report March 2015 The 2014 Annual Report presents a detailed overview of the accomplishments, progress, and future endeavors of the U.S. Department of Energy Former Worker Medical Screening Program. The report includes: Program Overview Program Implementation Program Accomplishments Future Endeavors Individual Project Descriptions Exams Conducted through the Former Worker Program

  11. 2013 Former Worker Medical Screening Program Annual Report | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Former Worker Medical Screening Program Annual Report 2013 Former Worker Medical Screening Program Annual Report February 2014 The 2013 Annual Report presents a detailed overview of the accomplishments, progress, and future endeavors of the U.S. Department of Energy Former Worker Medical Screening Program. The report includes: Program Overview Program Implementation Program Accomplishments Individual Project Descriptions Exams Conducted through the Former Worker Program Program

  12. FIELD SCREENING FOR HALOGENATED VOLATILE ORGANIC COMPOUNDS

    SciTech Connect (OSTI)

    John F. Schabron; Joseph F. Rovani, Jr.; Theresa M. Bomstad

    2003-07-01

    Western Research Institute (WRI) is continuing work toward the development of new screening methodology and a test kit to measure halogenated volatile organic compounds (VOCs) in the field. Heated diode and corona discharge sensors are commonly used to detect leaks of refrigerants from air conditioners, freezers, and refrigerators. They are both selective to the presence of halogens. In prior work, the devices were tested for response to carbon tetrachloride, heptane, toluene, and water vapors. In the current work, sensor response was evaluated with sixteen halogenated VOCs relative to carbon tetrachloride. The results show that the response of the various chlorinated VOCs is within an order of magnitude of the response to carbon tetrachloride for each of the sensors. Thus, for field screening a single response factor can be used. Both types of leak detectors are being further modified to provide an on-board LCD signal readout, which is related to VOC concentration. The units will be fully portable and will operate with 115-V line or battery power. Signal background, noise level, and response data on the Bacharach heated diode detector and the TIF corona discharge detector show that when the response curves are plotted against the log of concentration, the plot is linear to the upper limit for the particular unit, with some curvature at lower levels. When response is plotted directly against concentration, the response is linear at the low end and is curved at the high end. The dynamic ranges for carbon tetrachloride of the two devices from the lower detection limit (S/N=2) to signal saturation are 4-850 vapor parts per million (vppm) for the corona discharge unit and 0.01-70 vppm for the heated diode unit. Additional circuit modifications are being made to lower the detection limit and increase the dynamic response range of the corona discharge unit. The results indicate that both devices show potential utility for future analytical method development work toward

  13. Low-effect HCP Screening Form | Open Energy Information

    Open Energy Info (EERE)

    HCP Screening FormLegal Published NA Year Signed or Took Effect 2014 Legal Citation Not provided DOI Not Provided Check for DOI availability: http:crossref.org...

  14. Former Worker Medical Screening Program 2015 Annual Report

    Energy Savers [EERE]

    BeLPT Beryllium Lymphocyte Proliferation Test BTMed Building Trades National Medical ... Security Site (formerly known as Nevada Test Site) NSSP National Supplemental Screening ...

  15. Former Worker Medical Screening Program 2014 Annual Report, March...

    Office of Environmental Management (EM)

    BeLPT Beryllium Lymphocyte Proliferation Test BTMed Building Trades National Medical ... Security Site (formerly known as Nevada Test Site) NSSP National Supplemental Screening ...

  16. SCREENING METHODS FOR SELECTION OF SURFACTANT FORMULATIONS FOR...

    Office of Scientific and Technical Information (OSTI)

    developing rapid screening methods to assess surfactant performance for IOR (Improved Oil Recovery) from fractured carbonate reservoirs. The desired outcome is to identify...

  17. Screening of Electrode Materials & Cell Chemistries and Streamlining...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 DOE Hydrogen and Fuel Cells Program, and Vehicle Technologies Program Annual Merit ... Screen Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrode ...

  18. Pre-Screening for Solar Projects on Federal Sites

    Broader source: Energy.gov [DOE]

    Conducting an agency-wide solar energy pre-screening is important prior to starting the project planning process when significant resources are expended.

  19. Roof screening for underground coal mines: recent developments

    SciTech Connect (OSTI)

    Compton, C.S.; Gallagher, S.; Molinda, G.M.; Mark, C.; Wilson, G.

    2008-06-15

    The use of screens to control falls of the immediate roof or roof skin (that is between the installed primary and secondary roof supports) is described. 5 figs.

  20. Computational catalyst screening: Scaling, bond-order and catalysis...

    Office of Scientific and Technical Information (OSTI)

    Computational catalyst screening: Scaling, bond-order and catalysis This content will become publicly available on December 29, 2017 Prev Next Title: Computational catalyst ...

  1. Visualizing the Behavior of Polar Domains and Screening Charges...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Visualizing the Behavior of Polar Domains and Screening Charges Under Electric and Mechanical Fields Event Sponsor: Mathematics and Computing Science - LANS Seminar Start Date: Sep...

  2. Appendix SCR: Feature, Event, and Process Screening for PA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SCR-2014 Feature, Event, and Process Screening for PA United States Department of Energy Waste Isolation Pilot Plant Carlsbad Field Office Carlsbad, New Mexico Compliance Recertification Application 2014 Appendix SCR-2014 Feature, Event, and Process Screening for PA Table of Contents SCR-1.0 Introduction SCR-2.0 Basis for FEPs Screening Process SCR-2.1 Requirement for FEPs SCR-2.2 FEPs List Development for the CCA SCR-2.3 Criteria for Screening of FEPs and Categorization of Retained FEPs

  3. Coal storage hopper with vibrating-screen agitator

    DOE Patents [OSTI]

    Daw, C.S.; Lackey, M.E.; Sy, R.L.

    1982-04-27

    The present invention is directed to a vibrating screen agitator in a coal storage hopper for assuring the uniform feed of coal having sufficient moisture content to effect agglomeration and bridging thereof in the coal hopper from the latter onto a conveyer mechanism. The vibrating scrren agitator is provided by a plurality of transversely oriented and vertically spaced apart screens in the storage hopper with a plurality of vertically oriented rods attached to the screens. The rods are vibrated to effect the vibration of the screens and the breaking up of agglomerates in the coal which might impede the uniform flow of the coal from the hopper onto a conveyer.

  4. Ecological Screening Values for Surface Water, Sediment, and...

    Office of Scientific and Technical Information (OSTI)

    Ecological Screening Values for Surface Water, Sediment, and Soil Friday, G. P. 54 ENVIRONMENTAL SCIENCES; SOILS; SURFACE WATERS; SEDIMENTS; ECOLOGICAL CONCENTRATION; ENVIRONMENTAL...

  5. FEMP Completes 2000th Renewable Energy Optimization Screening

    Broader source: Energy.gov [DOE]

    NREL's Renewable Energy Optimization (REopt) tool, developed through FEMP funding, is a screening tool that identifies and prioritizes cost-effective renewable energy opportunities at a single site...

  6. Project Screening and Design Toolkit | Open Energy Information

    Open Energy Info (EERE)

    Opportunities 3b.1. Assess technical potential for sector technologies Renewable Energy Technical Potential Toolkit Building Energy Assessment Toolkit Power System Screening...

  7. Apparatus and method for radioactive waste screening

    DOE Patents [OSTI]

    Akers, Douglas W.; Roybal, Lyle G.; Salomon, Hopi; Williams, Charles Leroy

    2012-09-04

    An apparatus and method relating to screening radioactive waste are disclosed for ensuring that at least one calculated parameter for the measurement data of a sample falls within a range between an upper limit and a lower limit prior to the sample being packaged for disposal. The apparatus includes a radiation detector configured for detecting radioactivity and radionuclide content of the of the sample of radioactive waste and generating measurement data in response thereto, and a collimator including at least one aperture to direct a field of view of the radiation detector. The method includes measuring a radioactive content of a sample, and calculating one or more parameters from the radioactive content of the sample.

  8. Astrophysical black holes in screened modified gravity

    SciTech Connect (OSTI)

    Davis, Anne-Christine; Jha, Rahul; Muir, Jessica; Gregory, Ruth E-mail: r.a.w.gregory@durham.ac.uk E-mail: jlmuir@umich.edu

    2014-08-01

    Chameleon, environmentally dependent dilaton, and symmetron gravity are three models of modified gravity in which the effects of the additional scalar degree of freedom are screened in dense environments. They have been extensively studied in laboratory, cosmological, and astrophysical contexts. In this paper, we present a preliminary investigation into whether additional constraints can be provided by studying these scalar fields around black holes. By looking at the properties of a static, spherically symmetric black hole, we find that the presence of a non-uniform matter distribution induces a non-constant scalar profile in chameleon and dilaton, but not necessarily symmetron gravity. An order of magnitude estimate shows that the effects of these profiles on in-falling test particles will be sub-leading compared to gravitational waves and hence observationally challenging to detect.

  9. Screening system and method of using same

    DOE Patents [OSTI]

    Jones, David A; Gresham, Christopher A; Basiliere, Marc L; Spates, James J; Rodacy, Philip J

    2014-04-15

    An integrated apparatus and method for screening an object for a target material is provided. The integrated apparatus comprises a housing and an integrated screener. The housing is positionable adjacent the object, and has a channel therethrough. The integrated screener is positionable in the housing, and comprises a fan, at least one filter, a heater and an analyzer. The fan is for drawing air carrying particles and vapor through the channel of the housing. The filter(s) is/are positionable in the channel of the housing for passage of the air therethrough. The filter(s) comprise(s) at least one metal foam having a plurality of pores therein for collecting and adsorbing a sample from the particles and vapor passing therethrough. The heater is for applying heat to the at least one metal foam whereby the collected sample is desorbed from the metal foam. The analyzer detects the target material from the desorbed sample.

  10. Screening of Electrode Materials & Cell Chemistries and Streamlining

    Broader source: Energy.gov (indexed) [DOE]

    Optimization of Electrodes | Department of Energy 1 DOE Hydrogen and Fuel Cells Program, and Vehicle Technologies Program Annual Merit Review and Peer Evaluation es028_lu_2011_p.pdf (3.23 MB) More Documents & Publications Screen Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrode Vehicle Technologies Office Merit Review 2015: Materials Benchmarking Activities for CAMP Facility Screen

  11. One piece microwave container screens for electrodeless lamps

    DOE Patents [OSTI]

    Turner, Brian; Ury, Michael

    1998-01-01

    A microwave powered electrodeless lamp includes an improved screen unit having mesh and solid sections with an internal reflector to reflect light into a light-transmitting chamber defined in the lamp microwave cavity by the reflector and the mesh section. A discharge envelope of a bulb is disposed in the light-transmitting chamber. Light emitted from the envelope is prevented by the reflector from entering the cavity portion bounded by the solid section of the screen. Replacing mesh material by solid metal material as part of the screen unit significantly reduces leakage of microwave energy from the lamp. The solid section has multiple compliant fingers defined therein for engaging the periphery of a flange on the waveguide unit so that a hose clamp can easily secure the screen to the assembly. Screen units of this type having different mesh section configurations can be interchanged in the lamp assembly to produce different respective illumination patterns.

  12. Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sykes, Robert W.; Gjersing, Erica L.; Foutz, Kirk; Rottmann, William H.; Kuhn, Sean A.; Foster, Cliff E.; Ziebell, Angela; Turner, Geoffrey B.; Decker, Stephen R.; Hinchee, Maud A. W.; et al

    2015-08-27

    In this study, lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. In this study, eucalyptus down-regulated in expression of cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H, EC 1.14.13.36) were evaluated for cell wall composition and reduced recalcitrance.

  13. Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release

    SciTech Connect (OSTI)

    Sykes, Robert W.; Gjersing, Erica L.; Foutz, Kirk; Rottmann, William H.; Kuhn, Sean A.; Foster, Cliff E.; Ziebell, Angela; Turner, Geoffrey B.; Decker, Stephen R.; Hinchee, Maud A. W.; Davis, Mark F.

    2015-08-27

    In this study, lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. In this study, eucalyptus down-regulated in expression of cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H, EC 1.14.13.36) were evaluated for cell wall composition and reduced recalcitrance.

  14. Synthesis and screening combinatorial arrays of zeolites

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy

    2003-11-18

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  15. Combinatorial screening of inorganic and organometallic materials

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy

    2002-01-01

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  16. Preparation and screening of crystalline inorganic materials

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy; Brice{hacek over }o, Gabriel; Sun, Xiao-Dong; Wang, Kai-An

    2008-10-28

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  17. Electrostatically screened, voltage-controlled electrostatic chuck

    DOE Patents [OSTI]

    Klebanoff, Leonard Elliott

    2001-01-01

    Employing an electrostatically screened, voltage-controlled electrostatic chuck particularly suited for holding wafers and masks in sub-atmospheric operations will significantly reduce the likelihood of contaminant deposition on the substrates. The electrostatic chuck includes (1) an insulator block having a outer perimeter and a planar surface adapted to support the substrate and comprising at least one electrode (typically a pair of electrodes that are embedded in the insulator block), (2) a source of voltage that is connected to the at least one electrode, (3) a support base to which the insulator block is attached, and (4) a primary electrostatic shield ring member that is positioned around the outer perimeter of the insulator block. The electrostatic chuck permits control of the voltage of the lithographic substrate; in addition, it provides electrostatic shielding of the stray electric fields issuing from the sides of the electrostatic chuck. The shielding effectively prevents electric fields from wrapping around to the upper or front surface of the substrate, thereby eliminating electrostatic particle deposition.

  18. ANALYSIS OF DUST DELIQUESCENCE FOR FEP SCREENING

    SciTech Connect (OSTI)

    C. Bryan

    2005-08-26

    The purpose of this report is to evaluate the potential for penetration of the Alloy 22 (UNS N06022) waste package outer barrier by localized corrosion due to the deliquescence of soluble constituents in dust present on waste package surfaces. The results support a recommendation to exclude deliquescence-induced localized corrosion (pitting or crevice corrosion) of the outer barrier from the total system performance assessment for the license application (TSPA-LA). Preparation of this report, and supporting laboratory studies and calculations, were performed as part of the planned effort in Work Package AEBM21, as implemented in ''Technical Work Plan for: Screening Evaluation for Dust Deliquescence and Localized Corrosion'' (BSC 2004 [DIRS 172804]), by Bechtel SAIC Company, LLC, and staff from three national laboratories: Sandia National Laboratories, Lawrence Livermore National Laboratory (LLNL), and Lawrence Berkeley National Laboratory (LBNL). The analysis and conclusions presented in this report are quality affecting, as determined in the controlling technical work plan. A summary of background information, based on work that was not performed under a quality assurance program, is provided as Appendix E. In this instance, the use of unqualified information is provided for transparency and corroboration only, and is clearly separated from uses of qualified information. Thus, the qualification status of this information does not affect the conclusions of this report. The acceptance criteria addressed in Sections 4.2 and 7.2 were changed from the technical work plan in response to review comments received during preparation of this report.

  19. Diagnosis and therapy for a recalcitrant parylene coater

    SciTech Connect (OSTI)

    Williams, J.M.; Rowen, J.T.

    1987-07-01

    An in-house built parylene coater produced coatings whose thicknesses varied 50% at the 1-..mu..m level, whose surfaces were rough and flapjacklike, and whose cross sections looked like foam. This paper details the product response to various equipment and operational changes. The systematic analysis showed that a balance of vacuum pressure and pyrolysis temperature is needed to produce good-quality coatings.

  20. Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals

    DOE Patents [OSTI]

    Brigmon, Robin L.; Story, Sandra; Altman, Denis J.; Berry, Christopher J.

    2011-05-03

    Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

  1. Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals

    DOE Patents [OSTI]

    Brigmon, Robin L.; Story, Sandra; Altman, Denis J.; Berry, Christopher J.

    2011-03-15

    Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

  2. Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals

    DOE Patents [OSTI]

    Brigmon, Robin L.; Story, Sandra; Altman; Denis J.; Berry, Christopher J.

    2011-03-29

    Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

  3. Surfactant biocatalyst for remediation of recalcitrant organics and heavy metals

    DOE Patents [OSTI]

    Brigmon, Robin L.; Story, Sandra; Altman, Denis; Berry, Christopher J.

    2009-01-06

    Novel strains of isolated and purified bacteria have been identified which have the ability to degrade petroleum hydrocarbons including a variety of PAHs. Several isolates also exhibit the ability to produce a biosurfactant. The combination of the biosurfactant-producing ability along with the ability to degrade PAHs enhances the efficiency with which PAHs may be degraded. Additionally, the biosurfactant also provides an additional ability to bind heavy metal ions for removal from a soil or aquatic environment.

  4. Photodetachment of hydrogen negative ions with screened Coulomb interaction

    SciTech Connect (OSTI)

    Zhang, Song Bin; Chen, Xiang Jun; Wang, Jian Guo; Janev, R. K.; Qu, Yi Zhi

    2010-06-15

    The effects of Coulomb interaction screening on photodetachment cross sections of hydrogen negative ions below the n =2 excitation threshold is investigated by using the R-matrix method with pseudostates. The contributions of Feshbach and shape resonances to H{sup -} photodetachment cross section are presented when screening length (D) varies from D = {infinity} to D = 4.6 a.u. It is found that the interaction screening has dramatic effects on the photodetachment cross sections of hydrogen negative ions in the photoelectron energy region around the n = 2 excitation threshold by strongly affecting the evolution of near-threshold resonances.

  5. Improved screen-bowl centrifuge recovery using polymer injection technology

    SciTech Connect (OSTI)

    Burchett, R.T.; McGough, K.M.; Luttrell, G.H.

    2006-08-15

    The paper reports the improved screen-bowl centrifuge recovery process using polymer injection technology. Field test and economic analysis are also included in the paper. 3 refs., 3 figs., 1 tab.

  6. Absolute charge calibration of scintillating screens for relativistic electron detection

    SciTech Connect (OSTI)

    Buck, A.; Popp, A.; Schmid, K.; Karsch, S.; Krausz, F.; Zeil, K.; Jochmann, A.; Kraft, S. D.; Sauerbrey, R.; Cowan, T.; Schramm, U.; Hidding, B.; Kudyakov, T.; Sears, C. M. S.; Veisz, L.; Pawelke, J.

    2010-03-15

    We report on new charge calibrations and linearity tests with high-dynamic range for eight different scintillating screens typically used for the detection of relativistic electrons from laser-plasma based acceleration schemes. The absolute charge calibration was done with picosecond electron bunches at the ELBE linear accelerator in Dresden. The lower detection limit in our setup for the most sensitive scintillating screen (KODAK Biomax MS) was 10 fC/mm{sup 2}. The screens showed a linear photon-to-charge dependency over several orders of magnitude. An onset of saturation effects starting around 10-100 pC/mm{sup 2} was found for some of the screens. Additionally, a constant light source was employed as a luminosity reference to simplify the transfer of a one-time absolute calibration to different experimental setups.

  7. New screening process for potentially cancer causing chemicals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New screening process for potentially cancer causing chemicals Click to share on Facebook (Opens in new window) Click to share on Twitter (Opens in new window) Click to share on ...

  8. Fragment Screen against HIV Protease: Discovery of Two Allosteric...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Screen against HIV Protease. Chem. Biol. Drug Des. 75: 257-268. References Brenner, B., Routy, J-P., Quan, Y., Moisi, D., Oliveira, M., Turner, D., Wainberg, M. A. &...

  9. Screening Metal-Organic Frameworks by Analysis of Transient Breakthrou...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Screening Metal-Organic Frameworks by Analysis of Transient Breakthrough of Gas Mixtures in a Fixed Bed Adsorber Previous Next List Rajamani Krishna and Jeffrey R. Long, J. Phys....

  10. Former Worker Program Reaches 100,000 Screening Milestone | Department...

    Office of Environmental Management (EM)

    Exams are offered at clinics in communities near DOE sites, as well as through a large ... Addthis Related Articles Former Worker Program Reaches 100,000 Screening Milestone 2014 ...

  11. In silico screening of carbon-capture materials | Center for...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In silico screening of carbon-capture materials Previous Next List L.-C. Lin, A. H. Berger, R. L. Martin, J. Kim, J. A. Swisher, K. Jariwala, C. H. Rycroft, A. S. Bhown, M. W....

  12. John Day Fish Passage and Screening; 2003 Annual Report.

    SciTech Connect (OSTI)

    Allen, Steve

    2004-02-01

    The primary goal of the Oregon Screens Project was to implement 20 replacement screens projects in the John Day sub-basin and any projects identified in the Umatilla and Walla Walla sub-basins. A secondary goal is to complete a passage project, if one is identified, in any of the above sub-basins. Mid-Columbia ESU listed steelhead and USF&W listed bull trout inhabit these sub-basins and are present at most locations, along with a variety of resident fish species. We also provide assistance to our Enterprise Screen Shop, in the Grande Ronde/Imnaha sub-basins, if needed. All projects were designed and implemented under current National Marine Fisheries Service screening and passage criteria.

  13. 2013 Former Worker Medical Screening Program Annual Report

    Broader source: Energy.gov [DOE]

    2013 Former Worker Medical Screening Program (FWP) Annual Report highlighting the accomplishments of this program, which provides critical services to those who served and sacrificed through their work in the Department and its predecessor agencies.

  14. NETL Studies High Throughput Membrane Screening | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NETL Studies High Throughput Membrane Screening NETL Studies High Throughput Membrane Screening Membranes offer a potential low-maintenance and economical method for gas separations from power plant flue gas streams. Polymer membranes and supported liquid membranes show great promise to solve problems in the area of clean energy production. Carbon dioxide, a greenhouse gas, is a principal by-product of energy production from fossil fuels. Capturing CO2 from power plant flue gas streams is

  15. Beryllium Screening - Informed Choice Document | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Beryllium Screening - Informed Choice Document Beryllium Screening - Informed Choice Document January 2007 Cases of chronic beryllium disease (CBD), a legacy of the Department of Energy's (DOE) role in weapons production, have been increasing across the DOE complex. This trend has sparked increased concern about this serious occupational illness. In a national effort to identify current and former workers who have CBD or are sensitized to beryllium and to better understand the illness, DOE has

  16. Joint Outreach Task Group Former Workers Screening Program | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Former Workers Screening Program Joint Outreach Task Group Former Workers Screening Program The Joint Outreach Task Group (JOTG) includes representatives from DOE, Department of Labor (DOL), the National Institute for Occupational Safety and Health (NIOSH), the Offices of the Ombudsman for DOL and NIOSH, and the DOE-funded FWP projects. The JOTG was established in 2009 under the premise that agencies/programs with common goals can work together by combining resources and coordinating

  17. Distributed PV Interconnection Screening Procedures and Online Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Distributed PV Interconnection Screening Procedures and Online Tools" Joel Dickinson with Salt River Project Solar Initiatives Group August 27, 2014 2 Speakers Joel Dickinson Sr. Engineer Salt River Project Kristen Ardani Solar Analyst National Renewable Energy Laboratory (DGIC moderator) August 27th, 2014 Joel Dickinson, P.E. Sr. Engineer Solar Initiatives Distributed PV Interconnection Screening and Online Tools Salt River Project  Established in 1903 after Theodore Roosevelt signed

  18. Calculation of screening masses in a chiral quark model

    SciTech Connect (OSTI)

    Li Xiangdong; Li Hu; Shakin, C.M.; Sun Qing

    2004-10-01

    We consider a simple model for the coordinate-space vacuum polarization function which is often parametrized in terms of a screening mass. We discuss the circumstances in which the value m{sub sc}={pi}T is obtained for the screening mass. In the model considered here, that result is obtained when the momenta in the relevant vacuum polarization integral are small with respect to the first Matsubara frequency.

  19. New screening process for potentially cancer causing chemicals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New screening process for potentially cancer causing chemicals Click to share on Facebook (Opens in new window) Click to share on Twitter (Opens in new window) Click to share on Reddit (Opens in new window) Click to share on Pinterest (Opens in new window) A better way to screen chemicals that may cause cancer is under development. This image shows in two dimensions what Berkeley lab scientists are working to achieve in three dimensions: cultures containing diverse cell types (seen here using

  20. Generalized charge-screening in relativistic ThomasFermi model

    SciTech Connect (OSTI)

    Akbari-Moghanjoughi, M.

    2014-10-15

    In this paper, we study the charge shielding within the relativistic Thomas-Fermi model for a wide range of electron number-densities and the atomic-number of screened ions. A generalized energy-density relation is obtained using the force-balance equation and taking into account the Chandrasekhar's relativistic electron degeneracy pressure. By numerically solving a second-order nonlinear differential equation, the Thomas-Fermi screening length is investigated, and the results are compared for three distinct regimes of the solid-density, warm-dense-matter, and white-dwarfs (WDs). It is revealed that our nonlinear screening theory is compatible with the exponentially decaying Thomas-Fermi-type shielding predicted by the linear response theory. Moreover, the variation of relative Thomas-Fermi screening length shows that extremely dense quantum electron fluids are relatively poor charge shielders. Calculation of the total number of screening electrons around a nucleus shows that there is a position of maximum number of screening localized electrons around the screened nucleus, which moves closer to the point-like nucleus by increase in the plasma number density but is unaffected due to increase in the atomic-number value. It is discovered that the total number of screening electrons, (N{sub s}?r{sub TF}{sup 3}/r{sub d}{sup 3} where r{sub TF} and r{sub d} are the Thomas-Fermi and interparticle distance, respectively) has a distinct limit for extremely dense plasmas such as WD-cores and neutron star crusts, which is unique for all given values of the atomic-number. This is equal to saying that in an ultrarelativistic degeneracy limit of electron-ion plasma, the screening length couples with the system dimensionality and the plasma becomes spherically self-similar. Current analysis can provide useful information on the effects of relativistic correction to the charge screening for a wide range of plasma density, such as the inertial-confined plasmas and compact stellar

  1. Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons via Indirect Liquefaction. Thermochemical Research Pathway to High-Octane Gasoline Blendstock Through Methanol/Dimethyl Ether Intermediates

    SciTech Connect (OSTI)

    Tan, Eric C. D.; Talmadge, Michael; Dutta, Abhijit; Hensley, Jesse; Schaidle, Josh; Biddy, Mary; Humbird, David; Snowden-Swan, Lesley J.; Ross, Jeff; Sexton, Danielle; Yap, Raymond; Lukas, John

    2015-03-01

    This report was developed as part of the U.S. Department of Energy’s Bioenergy Technologies Office’s (BETO’s) efforts to enable the development of technologies for the production of infrastructure-compatible, cost-competitive liquid hydrocarbon fuels from lignocellulosic biomass feedstocks. The research funded by BETO is designed to advance the state of technology of biomass feedstock supply and logistics, conversion, and overall system sustainability. It is expected that these research improvements will be made within the 2022 timeframe. As part of their involvement in this research and development effort, the National Renewable Energy Laboratory and the Pacific Northwest National Laboratory investigate the economics of conversion pathways through the development of conceptual biorefinery process models and techno-economic analysis models. This report describes in detail one potential conversion process for the production of high-octane gasoline blendstock via indirect liquefaction of biomass. The processing steps of this pathway include the conversion of biomass to synthesis gas or syngas via indirect gasification, gas cleanup, catalytic conversion of syngas to methanol intermediate, methanol dehydration to dimethyl ether (DME), and catalytic conversion of DME to high-octane, gasoline-range hydrocarbon blendstock product. The conversion process configuration leverages technologies previously advanced by research funded by BETO and demonstrated in 2012 with the production of mixed alcohols from biomass. Biomass-derived syngas cleanup via reforming of tars and other hydrocarbons is one of the key technology advancements realized as part of this prior research and 2012 demonstrations. The process described in this report evaluates a new technology area for the downstream utilization of clean biomass-derived syngas for the production of high-octane hydrocarbon products through methanol and DME intermediates. In this process, methanol undergoes dehydration to

  2. John Day Fish Passage and Screening; 2002 Annual Report.

    SciTech Connect (OSTI)

    Hartlerode, Ray; Dabashinsky, Annette; Allen, Steve

    2003-01-28

    This project is necessary to insure that replacement of fish screening devices and fishways meet current NMFS design criteria for the protection of all salmonid life stages. The mission of the fish passage program in Northeast Oregon is to protect and enhance fish populations by assisting private landowners, public landowners, irrigation districts and others by maintaining fish screening devices and fishways. These facilities reduce or eliminate fish loss associated with irrigation withdrawals, and as a result insure fish populations are maintained for enjoyment by present and future generations. Assistance is provided through state and federal programs. This can range from basic technical advice to detailed construction, fabrication and maintenance of screening and passage facilities. John Day screens personnel identified 50 sites for fish screen replacement, and one fish passage project. These sites are located in critical spawning, rearing and migration areas for spring chinook, summer steelhead and bull trout. All projects were designed and implemented to meet current NMFS criteria. It is necessary to have a large number of sites identified due to changes in weather, landowner cooperation and access issues that come up as we try and implement our goal of 21 completed projects.

  3. Screen bowl centrifuge: a high-efficiency particle size separator

    SciTech Connect (OSTI)

    Mohanty, M.K.; Zhang, B.; Khanna, N.; Palit, A.; Dube, B.

    2008-05-15

    Over the years, screen bowl centrifuges have been widely used for dewatering fine coal in coal preparation plants in the United States and elsewhere. It is generally recognized in the engineering and scientific communities that screen bowl centrifuges provide some degree of particle size separation while dewatering fine coal in a common application. However, the extent of differential partitioning of coarse and fine particles achievable by a screen bowl centrifuge has not been systematically studied in the past. The present investigation was aimed at conducting a parametric study using a statistically designed experimental program to better understand and optimize the size classification performance of a screen bowl centrifuge. A continuously operating screen bowl centrifuge having a bowl diameter of 0.5 m was used for this study at the Illinois Coal Development Park. Three key operating parameters, i.e., feed flow rate, feed solid content and pool depth, were varied to conduct a total of 17 experiments using a three-level factorial test matrix. Some of the best size separation performances achieved in this study may be described as having an imperfection value of 0.13 at an effective separation size (d(50c)) of 38 mu m and an imperfection value of 0.27 at an effective separation size (d(50c)) of 2.8 mu m. Due to an effective separation of ultrafine high ash materials, the ash content of the screen bowl feed was reduced from 22.3% to a minimum of 8.84% with a combustible recovery of 84.1% and an ash rejection of 71.6%. A higher combustible recovery of 92.1% was achieved at a product ash content of 12.5% with a d(50c) of 2.8 mu m and imperfection of 0.27.

  4. Modeling granular phosphor screens by Monte Carlo methods

    SciTech Connect (OSTI)

    Liaparinos, Panagiotis F.; Kandarakis, Ioannis S.; Cavouras, Dionisis A.; Delis, Harry B.; Panayiotakis, George S.

    2006-12-15

    The intrinsic phosphor properties are of significant importance for the performance of phosphor screens used in medical imaging systems. In previous analytical-theoretical and Monte Carlo studies on granular phosphor materials, values of optical properties, and light interaction cross sections were found by fitting to experimental data. These values were then employed for the assessment of phosphor screen imaging performance. However, it was found that, depending on the experimental technique and fitting methodology, the optical parameters of a specific phosphor material varied within a wide range of values, i.e., variations of light scattering with respect to light absorption coefficients were often observed for the same phosphor material. In this study, x-ray and light transport within granular phosphor materials was studied by developing a computational model using Monte Carlo methods. The model was based on the intrinsic physical characteristics of the phosphor. Input values required to feed the model can be easily obtained from tabulated data. The complex refractive index was introduced and microscopic probabilities for light interactions were produced, using Mie scattering theory. Model validation was carried out by comparing model results on x-ray and light parameters (x-ray absorption, statistical fluctuations in the x-ray to light conversion process, number of emitted light photons, output light spatial distribution) with previous published experimental data on Gd{sub 2}O{sub 2}S:Tb phosphor material (Kodak Min-R screen). Results showed the dependence of the modulation transfer function (MTF) on phosphor grain size and material packing density. It was predicted that granular Gd{sub 2}O{sub 2}S:Tb screens of high packing density and small grain size may exhibit considerably better resolution and light emission properties than the conventional Gd{sub 2}O{sub 2}S:Tb screens, under similar conditions (x-ray incident energy, screen thickness)

  5. Laboratory to change vehicle traffic-screening regimen at vehicle

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    inspection station Changes to vehicle traffic-screening Laboratory to change vehicle traffic-screening regimen at vehicle inspection station Lanes two through five will be open 24 hours a day and won't be staffed by a Laboratory protective force officer. September 1, 2009 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy

  6. Smart Screening System (S3) In Taconite Processing

    SciTech Connect (OSTI)

    Daryoush Allaei; Asim Syed Mohammed; David Tarnowski

    2004-09-01

    The conventional vibrating machines used in processing plants have had undesirable high noise and vibration levels. They also have had unsatisfactorily low screening efficiency, high energy consumption, high maintenance cost, low productivity, and poor worker safety. These conventional vibrating machines have been used in most every processing plant. Most of the current material separation technology uses heavy and inefficient electric motors with an unbalance rotating mass to generate the shaking. In addition to being excessively noisy, inefficient, and high-maintenance, these vibrating machines are often the bottleneck in the entire process. Furthermore, these motors, along with the vibrating machines and supporting structure, shake other machines and structures in the vicinity. The latter increases maintenance costs while reducing worker health and safety. The conventional vibrating fine screens at taconite processing plants have had the same problems as those listed above. This has resulted in lower screening efficiency, higher energy and maintenance cost, and lower productivity and workers safety concerns. The focus of this work is on the design of a high performance screening machine suitable for taconite processing plants. SmartScreens{trademark} technology uses miniaturized motors, based on smart materials, to generate the shaking. The underlying technologies are Energy Flow Control{trademark} and Vibration Control by Confinement{trademark}. These concepts are used to direct energy flow and confine energy efficiently and effectively to the screen function. The SmartScreens{trademark} technology addresses problems related to noise and vibration, screening efficiency, productivity, and maintenance cost and worker safety. Successful development of SmartScreens{trademark} technology will bring drastic changes to the screening and physical separation industry. The final designs for key components of the SmartScreens{trademark} have been developed. The key

  7. Nonlocal exchange correlation in screened-exchange densityfunctional methods

    SciTech Connect (OSTI)

    Lee, Byounghak; Wang, Lin-Wang; Spataru, Catalin D.; Louie,Steven G.

    2007-04-22

    We present a systematic study on the exchange-correlationeffects in screened-exchange local density functional method. Toinvestigate the effects of the screened-exchange potential in the bandgap correction, we have compared the exchange-correlation potential termin the sX-LDA formalism with the self-energy term in the GWapproximation. It is found that the band gap correction of the sX-LDAmethod primarily comes from the downshift of valence band states,resulting from the enhancement of bonding and the increase of ionizationenergy. The band gap correction in the GW method, on the contrary, comesin large part from the increase of theconduction band energies. We alsostudied the effects of the screened-exchange potential in the totalenergy by investigating the exchange-correlation hole in comparison withquantum Monte Carlo calculations. When the Thomas-Fermi screening isused, the sX-LDA method overestimates (underestimates) theexchange-correlation hole in short (long) range. From theexchange-correlation energy analysis we found that the LDA method yieldsbetter absolute total energy than sX-LDA method.

  8. 2015 Former Worker Medical Screening Program Annual Report

    Broader source: Energy.gov [DOE]

    The 2015 Annual Report presents a detailed overview of the accomplishments, progress, and future endeavors of the U.S. Department of Energy Former Worker Medical Screening Program. The report includes Program Overview, Program Implementation, Program Findings, Individual Project Descriptions, Exams Conducted through the Former Worker Program, Exam Results, and Resources.

  9. 2015 Former Worker Medical Screening Program Annual Report

    Broader source: Energy.gov [DOE]

    EHSS is privileged to present the 2015 Former Worker Medical Screening Program (FWP) Annual Report highlighting the accomplishments of this program, which provides critical services to those who served and sacrificed through their work in the Department and its predecessor agencies.

  10. Two-phase anaerobic digestion of screened dairy manure

    SciTech Connect (OSTI)

    Lo, K.V.; Liao, P.H.

    1985-01-01

    The paper describes the operating results of a two-phase process that separate the acid-phase and methane-phase digestion of screened dairy manure under mesophilic temperature. Acidogenesis pretreatment prior to the methanogenic fixed-film reactor phase resulted in a significant increase in methane yield.

  11. Application of neural networks to waste site screening

    SciTech Connect (OSTI)

    Dabiri, A.E.; Kraft, T.; Hilton, J.M.

    1993-03-01

    Waste site screening requires knowledge of the actual concentrations of hazardous materials and rates of flow around and below the site with time. The present approach to site screening consists primarily of drilling, boreholes near contaminated site and chemically analyzing the extracted physical samples and processing the data. In addition, hydraulic and geochemical soil properties are obtained so that numerical simulation models can be used to interpret and extrapolate the field data. The objective of this work is to investigate the feasibility of using neural network techniques to reduce the cost of waste site screening. A successful technique may lead to an ability to reduce the number of boreholes and the number of samples analyzed from each borehole to properly screen the waste site. The analytic tool development described here is inexpensive because it makes use of neural network techniques that can interpolate rapidly and which can learn how to analyze data rather than having to be explicitly programmed. In the following sections, data collection and data analyses will be described, followed by a section on different neural network techniques used. The results will be presented and compared with mathematical model. Finally, the last section will summarize the research work performed and make several recommendations for future work.

  12. Screening of Electrode Materials & Cell Chemistries and Streamlining

    Broader source: Energy.gov (indexed) [DOE]

    Optimization of Electrodes | Department of Energy 2 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting es028_lu_2012_o.pdf (2.32 MB) More Documents & Publications Cell Fabrication Facility Team Production and Research Activities Screening of Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrodes Validation of

  13. USER S GUIDE FOR THE RANDOM DRUG SCREENING SYSTEM

    SciTech Connect (OSTI)

    McNeany, Karen I

    2013-12-01

    The Random Drug Screening System (RDSS) is a desktop computing application designed to assign nongameable drug testing dates to each member in a population of employees, within a specific time line. The program includes reporting capabilities, test form generation, unique test ID number assignment, and the ability to flag high-risk employees for a higher frequency of drug testing than the general population.

  14. Hazard screening application guide. Safety Analysis Report Update Program

    SciTech Connect (OSTI)

    1992-06-01

    The basic purpose of hazard screening is to group precesses, facilities, and proposed modifications according to the magnitude of their hazards so as to determine the need for and extent of follow on safety analysis. A hazard is defined as a material, energy source, or operation that has the potential to cause injury or illness in human beings. The purpose of this document is to give guidance and provide standard methods for performing hazard screening. Hazard screening is applied to new and existing facilities and processes as well as to proposed modifications to existing facilities and processes. The hazard screening process evaluates an identified hazards in terms of the effects on people, both on-site and off-site. The process uses bounding analyses with no credit given for mitigation of an accident with the exception of certain containers meeting DOT specifications. The process is restricted to human safety issues only. Environmental effects are addressed by the environmental program. Interfaces with environmental organizations will be established in order to share information.

  15. 2014 Former Worker Medical Screening Program Annual Report

    Broader source: Energy.gov [DOE]

    EHSS is privileged to present the 2014 Former Worker Medical Screening Program (FWP) Annual Report highlighting the accomplishments of this program, which provides critical services to those who served and sacrificed through their work in the Department and its predecessor agencies.

  16. Fatigue failure of regenerator screens in a high frequency Stirling engine

    SciTech Connect (OSTI)

    Hull, D.R.; Alger, D.L.; Moore, T.J.; Sheuermann, C.M.

    1987-03-01

    Failure of Stirling Space Power Demonstrator Engine (SPDE) regenerator screens was investigated. After several hours of operation the SPDE was shut down for inspection and upon removal of the regenerator screens, debris of an unknown origin was discovered along with considerable cracking of the screens in localized areas. Metallurgical analysis of the debris determined it to be cracked-off-deformed pieces of the 41 pm thickness Type 304 stainless steel wire screen. Scanning electron microscopy of the cracked screens revealed failures occurring at wire crossovers and fatigue striations on the fracture surface of the wires. Thus, the screen failure can be characterized as a fatigue failure of the wires. The crossovers were determined to contain a 30 percent reduction in wire thickness and a highly worked microstructure occurring from the manufacturing process of the wire screens. Later it was found that reduction in wire thickness occurred because the screen fabricator had subjected the screen to a light cold-roll process after weaving. Installation of this screen left a clearance in the regenerator allowing the screens to move. The combined effects of the reduction in wire thickness, stress concentration (caused by screen movement), and highly worked microstructure at the wire crossovers led to the fatigue failure of the screens.

  17. Compensatory mitigation and screening rules in environmental impact assessment

    SciTech Connect (OSTI)

    Macintosh, Andrew Waugh, Lauren

    2014-11-15

    Concerns about the effectiveness of environmental impact assessment (EIA) have prompted proposals to improve its performance by limiting the discretion of decision-makers in screening. To investigate whether such proposals are likely to generate the desired results, we conducted an evaluation of the screening process under the Australian government's EIA regime from its introduction on 16 July 2000 to 30 June 2013 (study period). Almost 1 in 5 ‘particular manner’ decisions—a type of screening decision under the regime—were found to be unlawful. The extent of non-compliance is explained on the basis of convenience. The department was required to assess a large number of projects under tight timeframes and with limited resources, while being pressured by proponents to allow their projects to bypass EIA. These pressures resulted in the development of an informal custom whereby the formal compensatory mitigation restrictions were frequently ignored. The results highlight the relative significance of formal and informal institutions in EIA. Formal EIA rules typically provide a mere outline of the process. The informal institutions adopted by administrators often have a greater influence on how the process operates and what it achieves. - Highlights: • Concerns about the effectiveness of environmental impact assessment (EIA) have prompted proposals to improve its performance by limiting the discretion of decision-makers in screening. • To investigate whether such proposals are likely to generate the desired results, we conducted an evaluation of the Australian government's screening process, looking at the extent of compliance with a formal prohibition on the consideration of compensatory mitigation. • Almost 1 in 5 ‘particular manner’ decisions – a type of screening decision under the regime – were found to be unlawful (with a 95% confidence interval of between 1:4 and 1:7) because of a failure to abide by the compensatory mitigation restrictions

  18. Bench-Top Engine System for Fast Screening of Alternative Fuels...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bench-Top Engine System for Fast Screening of Alternative Fuels and Fuel Additives Bench-Top Engine System for Fast Screening of Alternative Fuels and Fuel Additives A bench-top ...

  19. Argonne National Laboratory-West Former Workers, Construction Worker Screening Projects

    Broader source: Energy.gov [DOE]

    Argonne National Laboratory-West Former Construction Workers (now known as Idaho National Laboratory), Construction Worker Screening Projects

  20. In-situ characterization technique for screening contaminated soils

    SciTech Connect (OSTI)

    Jaselskis, E.J.; Anderson, M.S.; D`Silva, A.P.; Baldwin, D.P.; Zamzow, D.S.

    1995-07-01

    An innovative field sampling system for screening contaminated soils has been developed using laser ablation coupled with inductively coupled plasma-atomic emission spectrometry (LA-ICP-AES) technology. This sampling approach provides in-situ real-time analysis of trace inorganic elements and is conducted through a mobile testing facility that consists of an instrumentation vehicle called the Mobile Demonstration Laboratory for Environmental Screening Technologies (MDLEST) and an attached trailer called the Robotic Sampling Accessory (RSA). The RSA provides automated sampling capabilities through an attached three-degree-of-freedom robot that is equipped with a surface-sampling probe. The MDLEST-RSA was successfully tested at a Department of Energy (DOE) site in Fernald, Ohio, during the fall of 1992. This paper provides a description of the analysis technique, the MDLEST and RSA, and results of the field demonstration. In addition, benefits, limitations, and future plans are also discussed.

  1. From screen to structure with a harvestable microfluidic device

    SciTech Connect (OSTI)

    Stojanoff V.; Jakonic, J.; Oren, D.A.; Nagarajan, V.; Navarro Poulsen, J.C.; Adams-Cioaba, M.A.; Bergfors, T. and Sommer, M.O.A.

    2011-06-21

    Advances in automation have facilitated the widespread adoption of high-throughput vapor-diffusion methods for initial crystallization screening. However, for many proteins, screening thousands of crystallization conditions fails to yield crystals of sufficient quality for structural characterization. Here, the rates of crystal identification for thaumatin, catalase and myoglobin using microfluidic Crystal Former devices and sitting-drop vapour-diffusion plates are compared. It is shown that the Crystal Former results in a greater number of identified initial crystallization conditions compared with vapor diffusion. Furthermore, crystals of thaumatin and lysozyme obtained in the Crystal Former were used directly for structure determination both in situ and upon harvesting and cryocooling. On the basis of these results, a crystallization strategy is proposed that uses multiple methods with distinct kinetic trajectories through the protein phase diagram to increase the output of crystallization pipelines.

  2. INDUSTRIAL/MILITARY ACTIVITY-INITIATED ACCIDENT SCREENING ANALYSIS

    SciTech Connect (OSTI)

    D.A. Kalinich

    1999-09-27

    Impacts due to nearby installations and operations were determined in the Preliminary MGDS Hazards Analysis (CRWMS M&O 1996) to be potentially applicable to the proposed repository at Yucca Mountain. This determination was conservatively based on limited knowledge of the potential activities ongoing on or off the Nevada Test Site (NTS). It is intended that the Industrial/Military Activity-Initiated Accident Screening Analysis provided herein will meet the requirements of the ''Standard Review Plan for the Review of Safety Analysis Reports for Nuclear Power Plants'' (NRC 1987) in establishing whether this external event can be screened from further consideration or must be included as a design basis event (DBE) in the development of accident scenarios for the Monitored Geologic Repository (MGR). This analysis only considers issues related to preclosure radiological safety. Issues important to waste isolation as related to impact from nearby installations will be covered in the MGR performance assessment.

  3. High Throughput/Combinatorial Screening of Hydrogen Storage Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (presentation) | Department of Energy Materials (presentation) High Throughput/Combinatorial Screening of Hydrogen Storage Materials (presentation) Presented at the U.S. Department of Energy's Hydrogen Storage Meeting held June 26, 2007 in Bethesda, Maryland. ht_symyx_boussie.pdf (1013.19 KB) More Documents & Publications High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint UC-Berkeley/Symyx DoD/DLA Project) (presentation) High

  4. Distributed PV Interconnection Screening Procedures and Online Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Screening Procedures and Online Tools Page 1 of 9 Kristen Ardani, Joel Dickinson, Max Berger, David Crowell, Jeff Dickinson, Kelly Webster Page 1 of 9 [Speaker: Kristen Ardani] Cover Slide: Thank you everyone for joining us today for the DG Interconnection Collaborative. My name is Kristen Ardani,I'm an analyst here at NREL and the lead facilitator of the DGIC. We are fortunate today to have speakers Joel Dickinson of Salt River Project. We are going to discuss distributed PV interconnection

  5. Cavity based furnace for wafer screening - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Solar Photovoltaic Solar Photovoltaic Find More Like This Return to Search Cavity based furnace for wafer screening National Renewable Energy Laboratory Contact NREL About This Technology NREL Principal Engineer Bhushan Sopori has fired up an optical furnace he developed to efficiently fabricate solar cells. NREL Principal Engineer Bhushan Sopori has fired up an optical furnace he developed to efficiently fabricate solar cells. Technology Marketing Summary The U.S. Department of Energy (DOE)

  6. Screen Electrode Materials and Cell Chemistries | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    09 DOE Hydrogen Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting, May 18-22, 2009 -- Washington D.C. esp_24_lu.pdf (1.35 MB) More Documents & Publications Screen Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrode Overcoming Processing Cost Barriers of High-Performance Lithium-Ion Battery Electrodes Overview of Applied Battery Research

  7. Power spectrum analysis for defect screening in integrated circuit devices

    DOE Patents [OSTI]

    Tangyunyong, Paiboon; Cole Jr., Edward I.; Stein, David J.

    2011-12-01

    A device sample is screened for defects using its power spectrum in response to a dynamic stimulus. The device sample receives a time-varying electrical signal. The power spectrum of the device sample is measured at one of the pins of the device sample. A defect in the device sample can be identified based on results of comparing the power spectrum with one or more power spectra of the device that have a known defect status.

  8. High Throughput/Combinatorial Screening of Hydrogen Storage Materials (presentation)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop HIGH THROUGHPUT/COMBINATORIAL SCREENING OF HYDROGEN STORAGE MATERIALS June 26, 2007 Tom Boussie Symyx Technologies Symyx develops and applies proprietary high-throughput research technologies and software to increase R&D efficiency in chemical, energy, electronics, pharmaceutical and academic labs. * Pioneer of High Throughput Research (HTR) for materials science * Founded in 1996; publicly traded since 1999 (SMMX: NASDAQ) * 400 Employees (mainly in Santa Clara, CA) * >$400

  9. Monitored retrievable storage facility site screening and evaluation report

    SciTech Connect (OSTI)

    none,

    1985-05-01

    The Nuclear Waste Policy Act of 1982 directs the Department of Energy to complete a detailed study of the need for and feasibility of, and to submit to the Congress a proposal for, the construction of one or more monitored retrievable storage facilities for high level radioactive waste and spent nuclear fuel.'' The Act directs that the proposal includes site specific designs. Further, the proposal is to include, for the first such facility, at least three alternative sites and at least five alternative combinations of such proposed site and facility designs...'' as well as a recommendation of the combination among the alternatives that the Secretary deems preferable.'' An MRS Site Screening Task Force has been formed to help identify and evaluated potential MRS facility sites within a preferred region and with the application of a siting process and criteria developed by the DOE. The activities of the task force presented in this report includes: site screening (Sections 3, 4, and 5), the MRS facilities which are to be sited are described; the criteria, process and outcome of the screening process is presented; and descriptions of the candidate MRS facility sites are given, and site evaluations (Sections 6 through 9) where the rational for the site evaluations are presented, along with each evaluation and findings of the Task Force.

  10. Self-cleaning inlet screen to an ocean riser pipe

    SciTech Connect (OSTI)

    Wetmore, S.B.; Person, A.

    1980-06-17

    A long, vertically disposed ocean water upwelling pipe, such as a cold water riser in an ocean thermal energy conversion facility, is fitted at its lower inlet end with a self-cleaning inlet screen. The screen includes a right conical frustum of loose metal netting connected at its larger upper end to the lower end of the pipe. A heavy, negatively buoyant closure is connected across the lower end of the frustum. A weight is suspended below the closure on a line which passes loosely through the closure into the interior of the screen. The line tends to stay stationary as the lower end of the pipe moves, as in response to ocean current vortex shedding and other causes, thus causing the closure to rattle on the line and to shake the netting. The included half-angle of the frustum is approximately 20 so that, on shaking of the netting, marine life accumulated on the netting becomes loose and falls free of the netting. 6 claims.

  11. Center for Lignocellulose Structure and Function - Symposium...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Cellulose-matrix interactions (Chaired by Charlie Anderson and Jeffrey Catchmark) Roger Newman, Scion, New Zealand; Physical analysis of primary and secondary cell walls Mike ...

  12. Fuel alcohol production from agricultural lignocellulosic feedstocks

    SciTech Connect (OSTI)

    Farina, G.E.; Barrier, J.W.; Forsythe, M.L. )

    1988-01-01

    A two-stage, low-temperature, ambient pressure, acid hydrolysis process that utilizes separate unit operations to convert hemicellulose and cellulose in agricultural residues and crops to fermentable sugars is being developed and tested. Based on the results of the bench-scale tests, an acid hydrolysis experimental plant to demonstrate the concepts of low-temperature acid hydrolysis on a much larger scale was built. Plant tests using corn stover have been conducted for more that a year and conversion efficiences have equaled those achieved in the laboratory. Laboratory tests to determine the potential for low-temperature acid hydrolysis of other feedstocks - including red clover, alfalfa, kobe lespedeza, winter rape, and rye grass - are being conducted. Where applicable, process modifications to include extraction before or after hydrolysis also are being studied. This paper describes the experimental plant and process, results obtained in the plant, results of alternative feedstocks testing in the laboratory, and a plan for an integrated system that will produce other fuels, feed, and food from crops grown on marginal land.

  13. BETO Webinar: Computational Studies of Lignocellulose Deconstruction...

    Energy Savers [EERE]

    Sugars and Biological Conversion of Sugars to Hydrocarbons High Level Computational Chemistry Approaches to the Prediction of Energetic Properties of Chemical Hydrogen Storage ...

  14. BETO Webinar: Computational Studies of Lignocellulose Deconstruction

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... 54, 597 4 - 5 nm 3 - 4 nm Crystalline core Fibril periphery Native plant-derived ... the cellulose assembly very stable "The stability of cellulose: a statistical perspective ...

  15. Process for the treatment of lignocellulosic biomass

    DOE Patents [OSTI]

    Dale, Bruce E.

    2014-07-08

    A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

  16. Genetic manipulation of lignocellulosic biomass for bioenergy...

    Office of Scientific and Technical Information (OSTI)

    GrantContract Number: SC0008628 Type: Publisher's Accepted Manuscript Journal Name: Current Opinion in Chemical Biology Additional Journal Information: Journal Volume: 29; Journal ...

  17. Fuels and Chemicals from Lignocellulosic Biomass: Valorization...

    Office of Scientific and Technical Information (OSTI)

    Biomass: Valorization of Lignin Mike Kent Deconstruction Division Joint BioEnergy Institute Outline 1. Introduction: -fuels and chemicals from Ngnocellulosic biomass -need ...

  18. Processes for pretreating lignocellulosic biomass: A review

    SciTech Connect (OSTI)

    McMillan, J.D.

    1992-11-01

    This paper reviews existing and proposed pretreatment processes for biomass. The focus is on the mechanisms by which the various pretreatments act and the influence of biomass structure and composition on the efficacy of particular pretreatment techniques. This analysis is used to identify pretreatment technologies and issues that warrant further research.

  19. SSF Experimental Protocols -- Lignocellulosic Biomass Hydrolysis...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... 8.22.7 Sample size: See flask preparation instructions. 8.22.8 Sample storage: Store liquor samples in the freezer if the analysis is not going to be carried out within a day. ...

  20. Process for the treatment of lignocellulosic biomass

    DOE Patents [OSTI]

    Dale, Bruce E.; Lynd, Lee R.; Laser, Mark

    2013-03-12

    A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

  1. Metagenomic Profiling Reveals Lignocellulose Degrading System...

    Office of Scientific and Technical Information (OSTI)

    Language: English Subject: 99 GENERAL AND MISCELLANEOUS; WOOD; HEMICELLULOSE; FUNCTIONALS Asian longhorned beetle; wood; hemicellulose; nutrient-acquisition; insect gut; microbial; ...

  2. New Catalytic Conversion of Lignocellulosic Presentation for...

    Broader source: Energy.gov (indexed) [DOE]

    March 24, 2015 Conversion R&D Review Mike Lilga Asanga Padmaperuma, Deanna Auberry PNNL This presentation does not contain any proprietary, confidential, or otherwise restricted ...

  3. Advanced Millimeter-Wave Imaging Enhances Security Screening

    SciTech Connect (OSTI)

    Sheen, David M.; Bernacki, Bruce E.; McMakin, Douglas L.

    2012-01-12

    Millimeter-wave imaging is rapidly gaining acceptance for passenger screening at airports and other secured facilities. This paper details a number of techniques developed over the last several years including novel image reconstruction and display techniques, polarimetric imaging techniques, array switching schemes, as well as high frequency high bandwidth techniques. Implementation of some of these methods will increase the cost and complexity of the mm-wave security portal imaging systems. RF photonic methods may provide new solutions to the design and development of the sequentially switched linear mm-wave arrays that are the key element in the mm-wave portal imaging systems.

  4. SLCA/IP power alternative screening method (SPASM)

    SciTech Connect (OSTI)

    Palmer, S.C. |; Ancrile, J.D.

    1995-03-01

    This report describes the SLCA/IP Power Alternative Screening Method (SPASM), which was used to screen 784 possible combinations of electric power marketing alternatives and dam operational scenarios to provide a representative range for analysis in the Western Area Power Administration Salt Lake City Area Integrated Projects (SLCA/IP) Electric Power Marketing Environmental Impact Statement (EIS). Each combination consists of one energy and capacity commitment level and one operational scenario for each of the hydroelectric facilities at Glen Canyon Dam, Flaming Gorge Dam, and the Aspinall Unit. The total annual cost to the SLCA/IP firm power customers of each of the 784 combinations is estimated and included in a relative frequency distribution. A relative frequency distribution is also generated for each marketing alternative. The number of combinations is reduced to 12 by taking the mean value and endpoint value for each of four marketing alternatives. Some minor deviations from this procedure, which are made for political purposes, are explained. 9 figs., 14 tabs.

  5. Automated video screening for unattended background monitoring in dynamic environments.

    SciTech Connect (OSTI)

    Carlson, Jeffrey J.

    2004-03-01

    This report addresses the development of automated video-screening technology to assist security forces in protecting our homeland against terrorist threats. A threat of specific interest to this project is the covert placement and subsequent remote detonation of bombs (e.g., briefcase bombs) inside crowded public facilities. Different from existing video motion detection systems, the video-screening technology described in this report is capable of detecting changes in the static background of an otherwise, dynamic environment - environments where motion and human activities are persistent. Our goal was to quickly detect changes in the background - even under conditions when the background is visible to the camera less than 5% of the time. Instead of subtracting the background to detect movement or changes in a scene, we subtracted the dynamic scene variations to produce an estimate of the static background. Subsequent comparisons of static background estimates are used to detect changes in the background. Detected changes can be used to alert security forces of the presence and location of potential threats. The results of this research are summarized in two MS Power-point presentations included with this report.

  6. Technology recommendations for pre-screening of IAEA swipe samples

    SciTech Connect (OSTI)

    Steeb, Jennifer L.; Smith, Nicholas A.; Lee, Denise L.; Huckabay, Heath A.; Ticknor, Brian W.

    2015-01-01

    Argonne and Oak Ridge National Laboratories have prepared an analysis of recommended, possible, and not recommended technologies for pre-screening and prioritizing IAEA swipes. The analytical techniques listed under the recommended technology list are the most promising techniques available to date. The recommended list is divided into two sections: Argonne’s recommended techniques and Oak Ridge’s recommended techniques. This list was divided based upon the expertise of staff in each subject area and/or the instrumentation available at each laboratory. The following section, titled Possible Techniques, is a list of analytical techniques that could be used for pre-screening and prioritizing swipes if additional instrumentation and effort were provided. These techniques are not necessarily top priority, but should not be discounted for future or expanded efforts. Lastly, a list of not recommended techniques is provided to outline the analytical methods and instrumentation that were investigated by each lab but deemed not suitable for this task. In addition to the recommendation list, a short procedure is provided outlining the steps followed for destructive analysis by the Network of Analytical Laboratories (NWAL) for determination of uranium concentrations, isotopic content of sample and swipe. Swipes generated for this project will be given to ORNL’s NWAL laboratory for analysis after analysis by other techniques at both laboratories.

  7. Characterization Report for the David Witherspoon Screen Art Site

    SciTech Connect (OSTI)

    Phyllis C. Weaver

    2011-01-31

    The U.S. Department of Energy (DOE) Oak Ridge Office (ORO) of Environmental Management (EM) requested the technical assistance of Oak Ridge Institute for Science and Education (ORISE) to characterize a tract of land associated with the David Witherspoon, Incorporated (DWI) Volunteer Equipment and Supply Company (VESC). This tract of land (hereinafter referred to as Screen Arts) is located in the Vestal Community in the 2000-block of Maryville Pike in south Knoxville, Tennessee, as shown in Figure A-1. This tract of land has been used primarily to store salvaged equipment and materials for resale, recycle, or for disposal in the former landfill once operated by DWI. The DWI Site industrial landfill and metal recycling business had been permitted by the Tennessee Division of Radiological Health to accept low-level radiologically contaminated metals. DWI received materials and equipment associated with operations from DOE sites, including those in Oak Ridge, Tennessee; Paducah, Kentucky; and Portsmouth, Ohio. It is likely that items stored at Screen Arts may have contained some residual radiological materials.

  8. Protein crystallization with microseed matrix screening: application to human germline antibody Fabs

    SciTech Connect (OSTI)

    Obmolova, Galina Malia, Thomas J.; Teplyakov, Alexey; Sweet, Raymond W.; Gilliland, Gary L.

    2014-07-23

    The power of microseed matrix screening is demonstrated in the crystallization of a panel of antibody Fab fragments. The crystallization of 16 human antibody Fab fragments constructed from all pairs of four different heavy chains and four different light chains was enabled by employing microseed matrix screening (MMS). In initial screening, diffraction-quality crystals were obtained for only three Fabs, while many Fabs produced hits that required optimization. Application of MMS, using the initial screens and/or refinement screens, resulted in diffraction-quality crystals of these Fabs. Five Fabs that failed to give hits in the initial screen were crystallized by cross-seeding MMS followed by MMS optimization. The crystallization protocols and strategies that resulted in structure determination of all 16 Fabs are presented. These results illustrate the power of MMS and provide a basis for developing future strategies for macromolecular crystallization.

  9. Experimental analysis of heat transfer characteristics and pressure drop through screen regenerative heat exchangers. Master's thesis

    SciTech Connect (OSTI)

    Wiese, J.L.

    1993-12-01

    This study investigated the effect on heat transfer and friction characteristics for screen regenerative heat exchangers with the screen thickness reduced by rolling. The experiments were performed on 250 and 325 mesh, 304 stainless steel screen using helium gas. Reynolds numbers, based on hydraulic radius, Re, were between 10 and 100. Both the Colburn factor, StPr(2/3), and friction factor, f, decreased as the screen thickness was reduced. A correlation was found for predicting friction factor, f. The drag coefficient per screen remained nearly unchanged for thicknesses reduced not more than 30 percent. The decrease in Colburn factor was significant for Re less than 40. For Re between 40 and 100 the decrease in Colburn factor was less than the experimental uncertainty. Regenerative Cooling, Regenerators, Cryogenic Engines, Screens (Woven Materials), Mesh, Stirling Cycle.

  10. Screenings and vertex operators of quantum superalgebra U{sub q}(sl-caret(N|1))

    SciTech Connect (OSTI)

    Kojima, Takeo

    2012-08-15

    We construct the screening currents of the quantum superalgebra U{sub q}(sl-caret(N|1)) for an arbitrary level k{ne}-N+ 1. We show that these screening currents commute with the superalgebra modulo total difference. We propose bosonizations of the vertex operators by using the screening currents. We check that these vertex operators are the intertwiners among the Fock-Wakimoto representation and the typical representation for rank N Less-Than-Or-Slanted-Equal-To 4.

  11. Screening of Potential Remediation Methods for the 200-ZP-1 Operable Unit at the Hanford Site

    SciTech Connect (OSTI)

    Truex, Michael J.; Nimmons, Michael J.; Johnson, Christian D.; Dresel, P EVAN.; Murray, Christopher J.

    2006-08-07

    A screening-level evaluation of potential remediation methods for application to the contaminants of concern (COC) in the 200-ZP-1 Operable Unit at the Hanford Site was conducted based on the methods outlined in the Guidance for Conducting Remedial Investigations and Feasibility Studies under CERCLA Interim Final. The scope of this screening was to identify the most promising remediation methods for use in the more detailed analysis of remediation alternatives that will be conducted as part of the full feasibility study. The screening evaluation was conducted for the primary COC (potential major risk drivers). COC with similar properties were grouped for the screening evaluation. The screening evaluation was conducted in two primary steps. The initial screening step evaluated potential remediation methods based on whether they can be effectively applied within the environmental setting of the 200-ZP-1 Operable Unit for the specified contaminants. In the second step, potential remediation methods were screened using scoping calculations to estimate the scale of infrastructure, overall quantities of reagents, and conceptual approach for applying the method for each defined grouping of COC. Based on these estimates, each method was screened with respect to effectiveness, implementability, and relative cost categories of the CERCLA feasibility study screening process defined in EPA guidance.

  12. Agenda from the U.S. Department of Energy's High Throughput Screening...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Screening of Hydrogen Storage Materials Workshop on June 26, 2007 High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint ...

  13. EERE Success Story—FEMP Completes 2000th Renewable Energy Optimization Screening

    Broader source: Energy.gov [DOE]

    NREL's Renewable Energy Optimization (REopt) tool, developed through FEMP funding, is a screening tool that identifies and prioritizes cost-effective renewable energy opportunities at a single site...

  14. Bench-Top Engine System for Fast Screening of Alternative Fuels and Fuel Additives

    Broader source: Energy.gov [DOE]

    A bench-top engine testing system was used to fast screen the efficiency of fuel additives or fuel blends on NOx reduction

  15. Region-to-area screening methodology for the Crystalline Repository Project

    SciTech Connect (OSTI)

    1985-04-01

    The purpose of this document is to describe the Crystalline Repository Project's (CRP) process for region-to-area screening of exposed and near-surface crystalline rock bodies in the three regions of the conterminous United States where crystalline rock is being evaluated as a potential host for the second nuclear waste repository (i.e., in the North Central, Northeastern, and Southeastern Regions). This document indicates how the US Department of Energy's (DOE) General Guidelines for the Recommendation of Sites for Nuclear Waste Repositories (10 CFR 960) were used to select and apply factors and variables for the region-to-area screening, explains how these factors and variable are to be applied in the region-to-area screening, and indicates how this methodology relates to the decision process leading to the selection of candidate areas. A brief general discussion of the screening process from the national survey through area screening and site recommendation is presented. This discussion sets the scene for detailed discussions which follow concerning the region-to-area screening process, the guidance provided by the DOE Siting Guidelines for establishing disqualifying factors and variables for screening, and application of the disqualifying factors and variables in the screening process. This document is complementary to the regional geologic and environmental characterization reports to be issued in the summer of 1985 as final documents. These reports will contain the geologic and environmental data base that will be used in conjunction with the methodology to conduct region-to-area screening.

  16. Method for screening inhibitors of the toxicity of Bacillus anthracis

    DOE Patents [OSTI]

    Cirino, Nick M.; Jackson, Paul J.; Lehnert, Bruce E.

    2001-01-01

    The protective antigen (PA) of Bacillus anthracis is integral to the mechanism of anthrax poisoning. The cloning, expression and purification of a 32 kDa B. anthracis PA fragment (PA32) is described. This fragment has also been expressed as a fusion construct to stabilized green fluorescent protein (EGFP-PA32). Both proteins were capable of binding to specific cell surface receptors as determined by fluorescent microscopy and a flow cytometric assay. To confirm binding specificity in the flow cytometric assay, non-fluorescent PA83 or PA32 was used to competitively inhibit fluorescent EGFP-PA32 binding to cell receptors. This assay can be employed as a rapid screen for compounds which disrupts binding of PA to cells. Additionally, the high intracellular expression levels and ease of purification make this recombinant protein an attractive vaccine candidate or therapeutic treatment for anthrax poisoning.

  17. Screening portal, system and method of using same

    DOE Patents [OSTI]

    Linker, Kevin L.; Hunter, John A.; Brusseau, Charles A.

    2013-04-30

    A portal, system and method for screening an object for a target substance is provided. The portal includes an inflatable bladder expandable to form a test space for receiving the object and a plurality of nozzles positioned about the inflatable bladder. The nozzles are in fluid communication with a fluid source for directing air over the object whereby samples are removed from the object for examination. A collector is operatively connected to the inflatable bladder for collecting the samples removed from the object. A detector is operatively connected to the collector for examining the removed samples for the presence of the target substance. At least one preconcentrator may be operatively connected to the collector for concentrating the samples collected thereby.

  18. Screening Experiments for Removal of Low-Level Tritiated Water

    SciTech Connect (OSTI)

    Kim, Yun Mi; Baney, Ronald; Powers, Kevin; Koopman, Ben; Tulenko, James

    2005-03-15

    Screening experiments for low levels of tritiated water (HTO) remediation based upon selective adsorption/desorption mechanisms utilizing equilibrium isotope effects have been carried out. Several organic and inorganic high surface area materials were investigated to assess their ability to selectively adsorb low concentrations of HTO. Ion-exchange resins with cation functionalities, chitosan, sodium alginate, and several inorganic media modified with metal cations exhibited promising results. Biomaterials, for example, chitosan and modified alginate, demonstrated positive results. Based on the literature and our preliminary testing, we postulate four possible mechanisms for selected tritium adsorption: hydrogen ion exchange, HTO coordination with surface cation sites, hydrogen bonding to surface basic sites, and secondary hydrogen bonding (structural water) in fine pores.

  19. Biomarkers of acute respiratory allergen exposure: Screening for sensitization potential

    SciTech Connect (OSTI)

    Pucheu-Haston, Cherie M.; Copeland, Lisa B.; Vallanat, Beena; Boykin, Elizabeth; Ward, Marsha D.W.

    2010-04-15

    Effective hazard screening will require the development of high-throughput or in vitro assays for the identification of potential sensitizers. The goal of this preliminary study was to identify potential biomarkers that differentiate the response to allergens vs non-allergens following an acute exposure in naive individuals. Female BALB/c mice received a single intratracheal aspiration exposure to Metarhizium anisopliae crude antigen (MACA) or bovine serum albumin (BSA) in Hank's Balanced Salt Solution (HBSS) or HBSS alone. Mice were terminated after 1, 3, 6, 12, 18 and 24 h. Bronchoalveolar lavage fluid (BALF) was evaluated to determine total and differential cellularity, total protein concentration and LDH activity. RNA was isolated from lung tissue for microarray analysis and qRT-PCR. MACA administration induced a rapid increase in BALF neutrophils, lymphocytes, eosinophils and total protein compared to BSA or HBSS. Microarray analysis demonstrated differential expression of genes involved in cytokine production, signaling, inflammatory cell recruitment, adhesion and activation in 3 and 12 h MACA-treated samples compared to BSA or HBSS. Further analyses allowed identification of approx 100 candidate biomarker genes. Eleven genes were selected for further assessment by qRT-PCR. Of these, 6 demonstrated persistently increased expression (Ccl17, Ccl22, Ccl7, Cxcl10, Cxcl2, Saa1), while C3ar1 increased from 6-24 h. In conclusion, a single respiratory exposure of mice to an allergenic mold extract induces an inflammatory response which is distinct in phenotype and gene transcription from the response to a control protein. Further validation of these biomarkers with additional allergens and irritants is needed. These biomarkers may facilitate improvements in screening methods.

  20. National Security Science and Technology Initiative: Air Cargo Screening

    SciTech Connect (OSTI)

    Bingham, Philip R; White, Tim; Cespedes, Ernesto; Bowerman, Biays; Bush, John

    2010-11-01

    The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security's Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with four of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009. The primary results of this effort are described in this document and can be summarized as follows: (1) Completed a gap analysis that identified threat signatures and observables, candidate technologies for detection, their current state of development, and provided recommendations for improvements to meet air cargo screening requirements. (2) Defined a Commodity/Threat/Detection matrix that focuses modeling and experimental efforts, identifies technology gaps and game-changing opportunities, and provides a means of summarizing current and emerging capabilities. (3) Defined key properties (e.g., elemental composition, average density, effective atomic weight) for basic commodity and explosive benchmarks, developed virtual models of the physical distributions (pallets) of three commodity types and three explosive

  1. A fisheries evaluation of the Wapato, Sunnyside, and Toppenish Creek canal fish screening facilities, spring 1988

    SciTech Connect (OSTI)

    Neitzel, D.A.; Abernethy, C.S.; Lusty, E.W.

    1990-03-01

    The Bonneville Power Administration, the United States Bureau of Reclamation, and the Washington State Department of Ecology are funding the construction and evaluation of fish passage and protection facilities at irrigation and hydroelectric diversions in the Yakima River Basin, Washington State. The programs provide offsite enhancement to compensate for fish and wildlife losses caused by hydroelectric development throughout the Columbia River Basin and address natural propagation of salmon to help mitigate the impact of irrigation in the Yakima River Basin. The Wapato, Sunnyside, and Toppenish Creek Screens are three of the facilities in the basin. This report evaluates the effectiveness of the screens in intercepting and returning juvenile salmonids unharmed to the river from which they were diverted. We evaluated the effectiveness of new screening facilities at the Toppenish Creek, Wapato, and Sunnyside canals in southcentral Washington State. Screen integrity tests indicated that fish released in front of the screens were prevented from entering the canal behind the screens. We conducted descaling tests at the Toppenish Creek Screens. We measured the time required for fish to move through the screen facilities. Methods used in 1988 were the same as those used at Sunnyside in 1985 and in subsequent years at Richland. Toppenish/Satus, and Wapato. 11 refs., 11 figs., 14 tabs.

  2. Biomolecular Science (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-04-01

    A brief fact sheet about NREL Photobiology and Biomolecular Science. The research goal of NREL's Biomolecular Science is to enable cost-competitive advanced lignocellulosic biofuels production by understanding the science critical for overcoming biomass recalcitrance and developing new product and product intermediate pathways. NREL's Photobiology focuses on understanding the capture of solar energy in photosynthetic systems and its use in converting carbon dioxide and water directly into hydrogen and advanced biofuels.

  3. High-Throughput Plasmid cDNA Library Screening

    SciTech Connect (OSTI)

    Wan, Kenneth H.; Yu, Charles; George, Reed A.; Carlson, JosephW.; Hoskins, Roger A.; Svirskas, Robert; Stapleton, Mark; Celniker, SusanE.

    2006-05-24

    Libraries of cDNA clones are valuable resources foranalysing the expression, structure, and regulation of genes, as well asfor studying protein functions and interactions. Full-length cDNA clonesprovide information about intron and exon structures, splice junctionsand 5'- and 3'-untranslated regions (UTRs). Open reading frames (ORFs)derived from cDNA clones can be used to generate constructs allowingexpression of native proteins and N- or C-terminally tagged proteins.Thus, obtaining full-length cDNA clones and sequences for most or allgenes in an organism is critical for understanding genome functions.Expressed sequence tag (EST) sequencing samples cDNA libraries at random,which is most useful at the beginning of large-scale screening projects.However, as projects progress towards completion, the probability ofidentifying unique cDNAs via EST sequencing diminishes, resulting in poorrecovery of rare transcripts. We describe an adapted, high-throughputprotocol intended for recovery of specific, full-length clones fromplasmid cDNA libraries in five days.

  4. Screening combinatorial arrays of inorganic materials with spectroscopy or microscopy

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy

    2004-02-03

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  5. Combinatorial synthesis and screening of non-biological polymers

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiao-Dong; Goldwasser, Isy; Briceno, Gabriel; Sun, Xiao-Dong; Wang, Kai-An

    2006-04-25

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  6. Fuel cell with metal screen flow-field

    DOE Patents [OSTI]

    Wilson, Mahlon S.; Zawodzinski, Christine

    2001-01-01

    A polymer electrolyte membrane (PEM) fuel cell is provided with electrodes supplied with a reactant on each side of a catalyzed membrane assembly (CMA). The fuel cell includes a metal mesh defining a rectangular flow-field pattern having an inlet at a first corner and an outlet at a second corner located on a diagonal from the first corner, wherein all flow paths from the inlet to the outlet through the square flow field pattern are equivalent to uniformly distribute the reactant over the CMA. In a preferred form of metal mesh, a square weave screen forms the flow-field pattern. In a particular characterization of the present invention, a bipolar plate electrically connects adjacent fuel cells, where the bipolar plate includes a thin metal foil having an anode side and a cathode side; a first metal mesh on the anode side of the thin metal foil; and a second metal mesh on the cathode side of the thin metal foil. In another characterization of the present invention, a cooling plate assembly cools adjacent fuel cells, where the cooling plate assembly includes an anode electrode and a cathode electrode formed of thin conducting foils; and a metal mesh flow field therebetween for distributing cooling water flow over the electrodes to remove heat generated by the fuel cells.

  7. Fuel cell with metal screen flow-field

    DOE Patents [OSTI]

    Wilson, Mahlon S.; Zawodzinski, Christine

    1998-01-01

    A polymer electrolyte membrane (PEM) fuel cell is provided with electrodes supplied with a reactant on each side of a catalyzed membrane assembly (CMA). The fuel cell includes a metal mesh defining a rectangular flow-field pattern having an inlet at a first corner and an outlet at a second corner located on a diagonal from the first corner, wherein all flow paths from the inlet to the outlet through the square flow field pattern are equivalent to uniformly distribute the reactant over the CMA. In a preferred form of metal mesh, a square weave screen forms the flow-field pattern. In a particular characterization of the present invention, a bipolar plate electrically connects adjacent fuel cells, where the bipolar plate includes a thin metal foil having an anode side and a cathode side; a first metal mesh on the anode side of the thin metal foil; and a second metal mesh on the cathode side of the thin metal foil. In another characterization of the present invention, a cooling plate assembly cools adjacent fuel cells, where the cooling plate assembly includes an anode electrode and a cathode electrode formed of thin conducting foils; and a metal mesh flow field therebetween for distributing cooling water flow over the electrodes to remove heat generated by the fuel cells.

  8. Fuel cell with metal screen flow-field

    DOE Patents [OSTI]

    Wilson, M.S.; Zawodzinski, C.

    1998-08-25

    A polymer electrolyte membrane (PEM) fuel cell is provided with electrodes supplied with a reactant on each side of a catalyzed membrane assembly (CMA). The fuel cell includes a metal mesh defining a rectangular flow-field pattern having an inlet at a first corner and an outlet at a second corner located on a diagonal from the first corner, wherein all flow paths from the inlet to the outlet through the square flow field pattern are equivalent to uniformly distribute the reactant over the CMA. In a preferred form of metal mesh, a square weave screen forms the flow-field pattern. In a particular characterization of the present invention, a bipolar plate electrically connects adjacent fuel cells, where the bipolar plate includes a thin metal foil having an anode side and a cathode side; a first metal mesh on the anode side of the thin metal foil; and a second metal mesh on the cathode side of the thin metal foil. In another characterization of the present invention, a cooling plate assembly cools adjacent fuel cells, where the cooling plate assembly includes an anode electrode and a cathode electrode formed of thin conducting foils; and a metal mesh flow field there between for distributing cooling water flow over the electrodes to remove heat generated by the fuel cells. 11 figs.

  9. White Oak Creek Embayment site characterization and contaminant screening analysis

    SciTech Connect (OSTI)

    Blaylock, B.G.; Ford, C.J.; Frank, M.L.; Hoffman, F.O.; Hook, L.A.

    1993-01-01

    Analyses of sediment samples collected near the mouth of White Oak Creek during the summer of 1990 revealed [sup 137]Cs concentrations [> 10[sup 6] Bq/kg dry wt (> 10[sup 4] pCi/g dry wt)] near the sediment surface. Available evidence indicates that these relatively high concentrations of [sup 137]Cs now at the sediment surface were released from White Oak Dam in the mid-1950s and had accumulated at depositionalsites in the embayment. These accumulated sediments are being eroded and transported downstream primarily during winter low-water levels by flood events and by a combination of normal downstream flow and the water turbulence created by the release of water from Melton Hill Dam during hydropower generation cycles. This report provides a more thorough characterization of the extent of contamination in WOCE than was previously available. Environmental samples collected from WOCE were analyzed for organic, inorganic, and radiological contaminants in fish, water, and sediment. These results were used to conduct a human health effects screening analysis. Walkover radiation surveys conducted inside the fenced area surrounding the WOCE at summer-pool (741 ft MSL) and at winter-pool (733 ft MSL) level, indicated a maximum exposure rate of 3 mR h[sup 1] 1 m above the soil surface.

  10. Monitored Retrievable Storage facility site screening and evaluation report

    SciTech Connect (OSTI)

    none,

    1985-05-01

    The Nuclear Waste Policy Act of 1982 directs the Department of Energy to complete a detailed study of the need for and feasibility of, and to submit to the Congress a proposal for, the construction of one or more monitored retrievable storage facilities for high level radioactive waste and spent nuclear fuel.'' The Act directs that the proposal includes site specific designs. Further, the proposal is to include, for the first such facility, at least three alternative sites and at least five alternative combinations of such proposed sites and facility designs {hor ellipsis}'' as well as a recommendation of the combination among the alternatives that the Secretary deems preferable.'' An MRS Site Screening Task Force has been formed to help identify and evaluate potential MRS facility sites within a preferred region and with the application of a siting process and criteria developed by the DOE. The activities of the Task Force presented in this report, all site evaluations (sections 13 through 16) where the rationale for the site evaluations are presented, along with each evaluation and findings of the Task Force. This is Volume 3 of a three volume document. References are also included in this volume.

  11. New mammography screen/film combinations: Imaging characteristics and radiation dose

    SciTech Connect (OSTI)

    Kimme-Smith, C.; Bassett, L.W.; Gold, R.H.; Zheutlin, J.; Gornbein, J.A. )

    1990-04-01

    Five types of film (Kodak OM, Kodak OM-SO177, Konica CM, Dupont Microvision, and Fuji MiMa) exposed in combination with seven different intensifying screens (Min R, Min R Medium, Siemens Orthox MA, Kyokka HR Mammo Fine, Agfa Gevaert Detail S (old and new), and Konica Monarch) were processed for either 90 sec (at 33.3{degrees}C) or 3 min (at 35.0 degrees C). The films imaged a Computerized Imaging Reference System phantom with additional detail test objects placed on its surface to produce four groups of objects with which to evaluate resolution and contrast. For objects that tested resolution, the Kyokka HR Mammo Fine (Fuji) screen was statistically significantly superior; for objects that tested contrast, the Konica Monarch screen was statistically significantly superior. Extended processing did not affect Dupont and Kodak OM film as much as it affected the other films. It did affect contrast for the other films tested. The mean glandular doses from gridless exposures ranged from 32 to 80 mrad (0.32-0.80 mGy) over all film/screen/processing combinations for a 4.5-cm-thick test object. Several new film/screen combinations can provide images superior to the Kodak Min R/OM combination at a reduced radiation dose. The Kyokka HR Mammo Fine (Fuji) screen was found statistically superior in radiographic resolution of mammographic test objects and the Konica Monarch screen was found to be superior in defining contrast.

  12. Evaluation of a standard test method for screening fuels in soils

    SciTech Connect (OSTI)

    Sorini, S.S.; Schabron, J.F.

    1996-12-31

    A new screening method for fuel contamination in soils was recently developed as American Society for Testing and Materials (ASTM) Method D-5831-95, Standard Test Method for Screening Fuels in Soils. This method uses low-toxicity chemicals and can be sued to screen organic- rich soils, as well as being fast, easy, and inexpensive to perform. Fuels containing aromatic compounds, such as diesel fuel and gasoline, as well as other aromatic-containing hydrocarbon materials, such as motor oil, crude oil, and cola oil, can be determined. The screening method for fuels in soils was evaluated by conducting a Collaborative study on the method. In the Collaborative study, a sand and an organic soil spiked with various concentrations of diesel fuel were tested. Data from the Collaborative study were used to determine the reproducibility (between participants) and repeatability (within participants) precision of the method for screening the test materials. The Collaborative study data also provide information on the performance of portable field equipment (patent pending) versus laboratory equipment for performing the screening method and a comparison of diesel concentration values determined using the screening method versus a laboratory method.

  13. Graphene based tunable fractal Hilbert curve array broadband radar absorbing screen for radar cross section reduction

    SciTech Connect (OSTI)

    Huang, Xianjun; Hu, Zhirun; Liu, Peiguo

    2014-11-15

    This paper proposes a new type of graphene based tunable radar absorbing screen. The absorbing screen consists of Hilbert curve metal strip array and chemical vapour deposition (CVD) graphene sheet. The graphene based screen is not only tunable when the chemical potential of the graphene changes, but also has broadband effective absorption. The absorption bandwidth is from 8.9GHz to 18.1GHz, ie., relative bandwidth of more than 68%, at chemical potential of 0eV, which is significantly wider than that if the graphene sheet had not been employed. As the chemical potential varies from 0 to 0.4eV, the central frequency of the screen can be tuned from 13.5GHz to 19.0GHz. In the proposed structure, Hilbert curve metal strip array was designed to provide multiple narrow band resonances, whereas the graphene sheet directly underneath the metal strip array provides tunability and averagely required surface resistance so to significantly extend the screen operation bandwidth by providing broadband impedance matching and absorption. In addition, the thickness of the screen has been optimized to achieve nearly the minimum thickness limitation for a nonmagnetic absorber. The working principle of this absorbing screen is studied in details, and performance under various incident angles is presented. This work extends applications of graphene into tunable microwave radar cross section (RCS) reduction applications.

  14. Argonne National Laboratory-West, Former Production Workers Screening Projects (now known as the Idaho National Laboratory)

    Broader source: Energy.gov [DOE]

    Argonne National Laboratory-West, Former Production Workers Screening Projects (now known as the Idaho National Laboratory)

  15. Letter Report: LAW Simulant Development for Cast Stone Screening Test

    SciTech Connect (OSTI)

    Russell, Renee L.; Westsik, Joseph H.; Swanberg, David J.; Eibling, Russell E.; Cozzi, Alex; Lindberg, Michael J.; Josephson, Gary B.; Rinehart, Donald E.

    2013-03-27

    testing program was developed in fiscal year (FY) 2012 describing in some detail the work needed to develop and qualify Cast Stone as a waste form for the solidification of Hanford LAW (Westsik et al. 2012). Included within Westsik et al. (2012) is a section on the near-term needs to address Tri-Party Agreement Milestone M-062-40ZZ. The objectives of the testing program to be conducted in FY 2013 and FY 2014 are to: • Determine an acceptable formulation for the LAW Cast Stone waste form. • Evaluate sources of dry materials for preparing the LAW Cast Stone. • Demonstrate the robustness of the Cast Stone waste form for a range of LAW compositions. • Demonstrate the robustness of the formulation for variability in the Cast Stone process. • Provide Cast Stone contaminant release data for PA and risk assessment evaluations. The first step in determining an acceptable formulation for the LAW Cast Stone waste form is to conduct screening tests to examine expected ranges in pretreated LAW composition, waste stream concentrations, dry-materials sources, and mix ratios of waste feed to dry blend. A statistically designed test matrix will be used to evaluate the effects of these key parameters on the properties of the Cast Stone as it is initially prepared and after curing. The second phase of testing will focus on selection of a baseline Cast Stone formulation for LAW and demonstrating that Cast Stone can meet expected waste form requirements for disposal in the IDF. It is expected that this testing will use the results of the screening tests to define a smaller suite of tests to refine the composition of the baseline Cast Stone formulation (e.g. waste concentration, water to dry mix ratio, waste loading).

  16. Theoretical Screening of Solid Sorbents for CO{sub 2} Capture

    SciTech Connect (OSTI)

    Duan, Y [NETL; Sorescu, D C [NETL; Luebke, D [NETL; Morreale, B [NETL; Li, B Y; Zhang, B; Johnson, J K; Zhang, K; Li, X S; King, D

    2013-04-11

    By combining thermodynamic database searches with density functional theory and lattice phonon dynamics, a screening methodology was developed to identify promising solid sorbent candidates for CO{sub 2} capture. This methodology has been used to screen hundreds of solid compounds and some of the promising candidates to date have been reported in literature. This screening methodology is particularly relevant for the case of materials for which experimental thermodynamic data is not available. Such areas of interest are represented by the case of solid mixtures and doped materials, where thermodynamic data are generally not available but for which the crystallographic structure is known or can be easily determined.

  17. High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins

    DOE Patents [OSTI]

    Kim, Sung-Hou; Kim, Rosalind; Jancarik, Jamila

    2012-01-31

    An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.

  18. Screening of the coulomb potential in a nondegenerate hydrogen isotope gas

    SciTech Connect (OSTI)

    Fedorovich, G.V. )

    1994-01-01

    To explain the mechanism of deuterium reactions in palladium and titanium (cold fusion), a model of an exotic deuterium plasma with possibly short nuclear distances due to thermal motion was considered. The screening parameter is increased by lowering the ion temperature. This is the usual feature of the screening phenomenon in plasma. Fully ionized gases of high density and low temperature are never possible outside the lattice. Hence, the growth of the screening parameter can be significant only for the hydrogen isotopes in the metal lattice. 21 refs., 3 figs.

  19. Technologies for pre-screening IAEA swipe samples

    SciTech Connect (OSTI)

    Smith, Nicholas A.; Steeb, Jennifer L.; Lee, Denise L.; Huckabay, Heath A.; Ticknor, Brian W.

    2015-11-09

    During the course of International Atomic Energy Agency (IAEA) inspections, many samples are taken for the purpose of verifying the declared facility activities and identifying any possible undeclared activities. One of these sampling techniques is the environmental swipe sample. Due to the large number of samples collected, and the amount of time that is required to analyze them, prioritizing these swipes in the field or upon receipt at the Network of Analytical Laboratories (NWAL) will allow sensitive or mission-critical analyses to be performed sooner. As a result of this study, technologies were placed into one of three categories: recommended, promising, or not recommended. Both neutron activation analysis (NAA) and X-ray fluorescence (XRF) are recommended for further study and possible field deployment. These techniques performed the best in initial trials for pre-screening and prioritizing IAEA swipes. We learned that for NAA more characterization of cold elements (such as calcium and magnesium) would need to be emphasized, and for XRF it may be appropriate to move towards a benchtop XRF versus a handheld XRF due to the increased range of elements available on benchtop equipment. Promising techniques that will require additional research and development include confocal Raman microscopy, fluorescence microscopy, and infrared (IR) microscopy. These techniques showed substantive responses to uranium compounds, but expensive instrumentation upgrades (confocal Raman) or university engagement (fluorescence microscopy) may be necessary to investigate the utility of the techniques completely. Point-and-shoot (handheld) Raman and attenuated total reflectance–infrared (ATR-IR) measurements are not recommended, as they have not shown enough promise to continue investigations.

  20. Hydroprocessing of solvent-refined coal: catalyst-screening results

    SciTech Connect (OSTI)

    Stiegel, G.J.; Tischer, R.E.; Polinski, L.M.

    1982-03-01

    This report presents the results of screening four catalysts for hydroprocessing a 50 wt% mixture of SRC-I in a prehydrogenated creosote oil using a continuous flow unit. All catalysts employed were nickel-molybdates with varying properties. Reaction conditions were 2000 psi, 8 SCFH of hydrogen, volume hourly space velocity of 0.6 to 1.0 cc of SRC-I/hr/cc of catalyst, and 48 hours at 750/sup 0/F followed by 72 hours at 780/sup 0/F. The results indicate that the Shell 324 catalyst is best for hydrogenation of the feedstock but only marginally better than CB 81-44 for denitrogenation. The CB 81-44 catalyst may be slightly better than Shell 324 for the conversion of the +850/sup 0/F fraction of the feedstock. Desulfurization was uniformly high for all catalysts. Catalysts with a bimodal pore size distribution (i.e., SMR7-6137(1)) appear to be better for denitrogenation than unimodal catalysts (i.e., SMR7-6137(4)) containing the same metals loading. Unimodal catalysts (i.e., Shell 324) with higher metals loadings are comparable to bimodal catalysts (i.e., CB 81-44) containing less metals. The results indicate that pore size distribution and metals loading are important parameters for high activity. Catalysts with a unimodal pore volume distribution are capable of being restored to their original state, while bimodal ones experience a loss in surface area and pore volume and an increase in pellet density. This is attributed to the more efficient use of the interior surface area of the catalyst, which results in higher accumulation of coke and metals. Since coke can be removed via controlled oxidation, the irreversible loss is due to the higher concentrations of metals in the catalyst.

  1. Managing System of Systems Requirements with a Requirements Screening Group

    SciTech Connect (OSTI)

    Ronald R. Barden

    2012-07-01

    Figuring out an effective and efficient way to manage not only your Requirements Baseline, but also the development of all your individual requirements during a Programs/Projects Conceptual and Development Life Cycle Stages can be both daunting and difficult. This is especially so when you are dealing with a complex and large System of Systems (SoS) Program with potentially thousands and thousands of Top Level Requirements as well as an equal number of lower level System, Subsystem and Configuration Item requirements that need to be managed. This task is made even more overwhelming when you have to add in integration with multiple requirements development teams (e.g., Integrated Product Development Teams (IPTs)) and/or numerous System/Subsystem Design Teams. One solution for tackling this difficult activity on a recent large System of Systems Program was to develop and make use of a Requirements Screening Group (RSG). This group is essentially a Team made up of co-chairs from the various Stakeholders with an interest in the Program of record that are enabled and accountable for Requirements Development on the Program/Project. The RSG co-chairs, often with the help of individual support team, work together as a Program Board to monitor, make decisions on, and provide guidance on all Requirements Development activities during the Conceptual and Development Life Cycle Stages of a Program/Project. In addition, the RSG can establish and maintain the Requirements Baseline, monitor and enforce requirements traceability across the entire Program, and work with other elements of the Program/Project to ensure integration and coordination.

  2. MRI screening for breast cancer in women at high risk; is the Australian breast MRI screening access program addressing the needs of women at high risk of breast cancer?

    SciTech Connect (OSTI)

    Schenberg, Tess; Mitchell, Gillian; Taylor, Donna; Saunders, Christobel

    2015-09-15

    Breast magnetic resonance imaging (MRI) screening of women under 50 years old at high familial risk of breast cancer was given interim funding by Medicare in 2009 on the basis that a review would be undertaken. An updated literature review has been undertaken by the Medical Services Advisory Committee but there has been no assessment of the quality of the screening or other screening outcomes. This review examines the evidence basis of breast MRI screening and how this fits within an Australian context with the purpose of informing future modifications to the provision of Medicare-funded breast MRI screening in Australia. Issues discussed will include selection of high-risk women, the options for MRI screening frequency and measuring the outcomes of screening.

  3. A Screen Space GPGPU Surface LIC Algorithm for Distributed Memory Data Parallel Sort Last Rendering Infrastructures

    SciTech Connect (OSTI)

    Loring, Burlen; Karimabadi, Homa; Rortershteyn, Vadim

    2014-07-01

    The surface line integral convolution(LIC) visualization technique produces dense visualization of vector fields on arbitrary surfaces. We present a screen space surface LIC algorithm for use in distributed memory data parallel sort last rendering infrastructures. The motivations for our work are to support analysis of datasets that are too large to fit in the main memory of a single computer and compatibility with prevalent parallel scientific visualization tools such as ParaView and VisIt. By working in screen space using OpenGL we can leverage the computational power of GPUs when they are available and run without them when they are not. We address efficiency and performance issues that arise from the transformation of data from physical to screen space by selecting an alternate screen space domain decomposition. We analyze the algorithm's scaling behavior with and without GPUs on two high performance computing systems using data from turbulent plasma simulations.

  4. The screening of 4f moments and delocalization in the compressed...

    Office of Scientific and Technical Information (OSTI)

    in the compressed light rare earths Citation Details In-Document Search Title: The screening of 4f moments and delocalization in the compressed light rare earths You are ...

  5. Black Bear Prep plant replaces high-frequency screens with fine wire sieves

    SciTech Connect (OSTI)

    Barbee, C.J.; Nottingham, J.

    2007-12-15

    At the Black Bear prep plant (near Wharncliffe, WV, USA) the clean coal from the spirals traditionally reported to high-frequency screens, which removed high-ash clay fines. Screens have inherent inefficiencies that allow clean coal to report to the screen underflow. The goal of this project was to capture the maximum amount of spiral clean coal while still removing the high-ash clay material found in the spiral product. The reduction of the circulating load and plant downtime for unscheduled maintenance were projected as additional benefits. After the plant upgrade, the maintenance related to the high frequency screens was eliminated and an additional 2.27 tons per hour (tph) of fine coal was recovered, which resulted in a payback period of less than one year. The article was adapted from a paper presented at Coal Prep 2007 in April 2007, Lexington, KY, USA. 1 ref., 1 fig., 1 tab.

  6. Screening Tool for Providers of Double-Stranded DNA - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Startup America Startup America Industrial Technologies Industrial Technologies Energy Analysis Energy Analysis Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Screening Tool for Providers of Double-Stranded DNA Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00227_ID2510 (2).pdf (829 KB) Technology Marketing SummaryThe invention screens purchase orders submitted to DNA synthesis

  7. Top Value Added Chemicals from Biomass: Volume I--Results of Screening for

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Potential Candidates from Sugars and Synthesis Gas | Department of Energy Value Added Chemicals from Biomass: Volume I--Results of Screening for Potential Candidates from Sugars and Synthesis Gas Top Value Added Chemicals from Biomass: Volume I--Results of Screening for Potential Candidates from Sugars and Synthesis Gas This report identifies twelve building block chemicals that can be produced from sugars via biological or chemical conversions. 35523.pdf (1.41 MB) More Documents &

  8. Top Value-Added Chemicals from Biomass - Volume II„Results of Screening

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for Potential Candidates from Biorefinery Lignin | Department of Energy Value-Added Chemicals from Biomass - Volume II„Results of Screening for Potential Candidates from Biorefinery Lignin Top Value-Added Chemicals from Biomass - Volume II„Results of Screening for Potential Candidates from Biorefinery Lignin This report evaluates lignins role as a renewable raw material resource. pnnl-16983.pdf (1006.18 KB) More Documents & Publications Low Cost Carbon Fiber from Renewable Resources

  9. Revolutionizing the Touch Screen? | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Revolutionizing the Touch Screen? News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science Highlights Presentations & Testimony News Archives Communications and Public Affairs Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 05.29.13 Revolutionizing the Touch Screen? Using nanotechnology, EFRC researchers fashion a new kind of transparent electrode

  10. Improved Algae-based Biorefining and High-throughput Screening of Algal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Photosynthetic Efficiency - Energy Innovation Portal Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Improved Algae-based Biorefining and High-throughput Screening of Algal Photosynthetic Efficiency University of Colorado Contact CU About This Technology Publications: PDF Document Publication CU2807B (Biorefining Flow Cytometer) Marketing Summary.pdf (164 KB) Technology Marketing Summary Improved Algae-based Biorefining and High-throughput Screening of Algal

  11. Flow method and apparatus for screening chemicals using micro x-ray fluorescence

    DOE Patents [OSTI]

    Warner, Benjamin P.; Havrilla, George J.; Miller, Thomasin C.; Lewis, Cris; Mahan, Cynthia A.; Wells, Cyndi A.

    2011-04-26

    Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.

  12. Flow method and apparatus for screening chemicals using micro x-ray fluorescence

    DOE Patents [OSTI]

    Warner, Benjamin P.; Havrilla, George J.; Miller, Thomasin C.; Lewis, Cris; Mahan, Cynthia A.; Wells, Cyndi A.

    2009-04-14

    Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow-separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.

  13. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7 UOP LLC. All rights reserved. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches High Throughput/Combinatorial Analysis of Hydrogen Storage Materials Meeting Organized by DOE on June 26, 2007 Adriaan Sachtler High Throughput/Combinatorial Analysis of Hydrogen Storage Materials Meeting Organized by DOE on June 26, 2007 Adriaan Sachtler © 2007 UOP LLC. All rights reserved. 2

  14. Colloid-based multiplexed method for screening plant biomass-degrading glycoside hydrolase activities in microbial communities

    SciTech Connect (OSTI)

    Reindl, W.; Deng, K.; Gladden, J.M.; Cheng, G.; Wong, A.; Singer, S.W.; Singh, S.; Lee, J.-C.; Yao, J.-S.; Hazen, T.C.; Singh, A.K; Simmons, B.A.; Adams, P.D.; Northen, T.R.

    2011-05-01

    The enzymatic hydrolysis of long-chain polysaccharides is a crucial step in the conversion of biomass to lignocellulosic biofuels. The identification and characterization of optimal glycoside hydrolases is dependent on enzyme activity assays, however existing methods are limited in terms of compatibility with a broad range of reaction conditions, sample complexity, and especially multiplexity. The method we present is a multiplexed approach based on Nanostructure-Initiator Mass Spectrometry (NIMS) that allowed studying several glycolytic activities in parallel under diverse assay conditions. Although the substrate analogs carried a highly hydrophobic perfluorinated tag, assays could be performed in aqueous solutions due colloid formation of the substrate molecules. We first validated our method by analyzing known {beta}-glucosidase and {beta}-xylosidase activities in single and parallel assay setups, followed by the identification and characterization of yet unknown glycoside hydrolase activities in microbial communities.

  15. Rigorous Screening Technology for Identifying Suitable CO2 Storage Sites II

    SciTech Connect (OSTI)

    George J. Koperna Jr.; Vello A. Kuuskraa; David E. Riestenberg; Aiysha Sultana; Tyler Van Leeuwen

    2009-06-01

    This report serves as the final technical report and users manual for the 'Rigorous Screening Technology for Identifying Suitable CO2 Storage Sites II SBIR project. Advanced Resources International has developed a screening tool by which users can technically screen, assess the storage capacity and quantify the costs of CO2 storage in four types of CO2 storage reservoirs. These include CO2-enhanced oil recovery reservoirs, depleted oil and gas fields (non-enhanced oil recovery candidates), deep coal seems that are amenable to CO2-enhanced methane recovery, and saline reservoirs. The screening function assessed whether the reservoir could likely serve as a safe, long-term CO2 storage reservoir. The storage capacity assessment uses rigorous reservoir simulation models to determine the timing, ultimate storage capacity, and potential for enhanced hydrocarbon recovery. Finally, the economic assessment function determines both the field-level and pipeline (transportation) costs for CO2 sequestration in a given reservoir. The screening tool has been peer reviewed at an Electrical Power Research Institute (EPRI) technical meeting in March 2009. A number of useful observations and recommendations emerged from the Workshop on the costs of CO2 transport and storage that could be readily incorporated into a commercial version of the Screening Tool in a Phase III SBIR.

  16. Water Velocity Measurements on a Vertical Barrier Screen at the Bonneville Dam Second Powerhouse

    SciTech Connect (OSTI)

    Hughes, James S.; Deng, Zhiqun; Weiland, Mark A.; Martinez, Jayson J.; Yuan, Yong

    2011-11-22

    Fish screens at hydroelectric dams help to protect rearing and migrating fish by preventing them from passing through the turbines and directing them towards the bypass channels by providing a sweeping flow parallel to the screen. However, fish screens may actually be harmful to fish if they become impinged on the surface of the screen or become disoriented due to poor flow conditions near the screen. Recent modifications to the vertical barrier screens (VBS) at the Bonneville Dam second powerhouse (B2) intended to increase the guidance of juvenile salmonids into the juvenile bypass system (JBS) have resulted in high mortality and descaling rates of hatchery subyearling Chinook salmon during the 2008 juvenile salmonid passage season. To investigate the potential cause of the high mortality and descaling rates, an in situ water velocity measurement study was conducted using acoustic Doppler velocimeters (ADV) in the gatewell slot at Units 12A and 14A of B2. From the measurements collected the average approach velocity, sweep velocity, and the root mean square (RMS) value of the velocity fluctuations were calculated. The approach velocities measured across the face of the VBS varied but were mostly less than 0.3 m/s. The sweep velocities also showed large variances across the face of the VBS with most measurements being less than 1.5 m/s. This study revealed that the approach velocities exceeded criteria recommended by NOAA Fisheries and Washington State Department of Fish and Wildlife intended to improve fish passage conditions.

  17. Chemical compatibility screening results of plastic packaging to mixed waste simulants

    SciTech Connect (OSTI)

    Nigrey, P.J.; Dickens, T.G.

    1995-12-01

    We have developed a chemical compatibility program for evaluating transportation packaging components for transporting mixed waste forms. We have performed the first phase of this experimental program to determine the effects of simulant mixed wastes on packaging materials. This effort involved the screening of 10 plastic materials in four liquid mixed waste simulants. The testing protocol involved exposing the respective materials to {approximately}3 kGy of gamma radiation followed by 14 day exposures to the waste simulants of 60 C. The seal materials or rubbers were tested using VTR (vapor transport rate) measurements while the liner materials were tested using specific gravity as a metric. For these tests, a screening criteria of {approximately}1 g/m{sup 2}/hr for VTR and a specific gravity change of 10% was used. It was concluded that while all seal materials passed exposure to the aqueous simulant mixed waste, EPDM and SBR had the lowest VTRs. In the chlorinated hydrocarbon simulant mixed waste, only VITON passed the screening tests. In both the simulant scintillation fluid mixed waste and the ketone mixture simulant mixed waste, none of the seal materials met the screening criteria. It is anticipated that those materials with the lowest VTRs will be evaluated in the comprehensive phase of the program. For specific gravity testing of liner materials the data showed that while all materials with the exception of polypropylene passed the screening criteria, Kel-F, HDPE, and XLPE were found to offer the greatest resistance to the combination of radiation and chemicals.

  18. Screening of Potential Remediation Methods for the 200-BP-5 Operable Unit at the Hanford Site

    SciTech Connect (OSTI)

    Truex, Michael J.; Dresel, P. EVAN; Nimmons, Michael J.; Johnson, Christian D.

    2006-09-21

    A screening-level evaluation of potential remediation methods for application to the contaminants of concern (COC) in the 200-BP-5 Operable Unit at the Hanford Site was conducted based on the methods outlined in the Guidance for Conducting Remedial Investigations and Feasibility Studies under CERCLA Interim Final (EPA 1988). The scope of this screening was to identify the most promising remediation methods for use in the more detailed analysis of remediation alternatives that will be conducted as part of the full feasibility study. The screening evaluation was conducted for the primary COC (potential major risk drivers) identified in the groundwater sampling and analysis plan for the operable unit (DOE/RL-2001-49, Rev. 1) with additions.

  19. First-principles thermodynamic screening approach to photo-catalytic water splitting with co-catalysts

    SciTech Connect (OSTI)

    Oberhofer, Harald; Reuter, Karsten

    2013-07-28

    We adapt the computational hydrogen electrode approach to explicitly account for photo-generated charges and use it to computationally screen for viable catalyst/co-catalyst combinations for photo-catalytic water splitting. The hole energy necessary to thermodynamically drive the reaction is employed as descriptor for the screening process. Using this protocol and hybrid-level density-functional theory, we show that water oxidation on bare TiO{sub 2} surfaces is thermodynamically more complex than previously thought. This motivates a screening for suitable co-catalysts for this half-reaction, which we carry out for Au particles down to the non-scalable size regime. We find that almost all small Au clusters studied are better suited for water photo-oxidation than an extended Au(111) surface or bare TiO{sub 2} facets.

  20. Anomalous QCD contribution to the Debye screening in an external field via holography

    SciTech Connect (OSTI)

    Gorsky, A.; Kopnin, P. N.; Krikun, A.

    2011-03-15

    In this paper we discuss the QCD contribution to the Abelian Debye and magnetic screening masses in a deconfined QCD plasma at finite temperature in the presence of an external magnetic field B. We use a holographic AdS/QCD setup in an AdS Schwarzschild black hole background and show that the electric screening mass has a form similar to the one-loop result in QED. Moreover, we calculate the corrections due to the magnetic field to all orders of B and demonstrate that in the case when the magnetic field is large the Debye mass grows linearly with B, while the magnetic screening mass vanishes. The whole effect of the magnetic field turns out to stem from the Chern-Simons action. We also discuss the zero temperature case in the chiral perturbation theory.

  1. Reevaluation of 1999 Health-Based Environmental Screening Levels (HBESLs) for Chemical Warfare Agents

    SciTech Connect (OSTI)

    Watson, Annetta Paule; Dolislager, Fredrick G

    2007-05-01

    This report evaluates whether new information and updated scientific models require that changes be made to previously published health-based environmental soil screening levels (HBESLs) and associated environmental fate/breakdown information for chemical warfare agents (USACHPPM 1999). Specifically, the present evaluation describes and compares changes that have been made since 1999 to U.S. Environmental Protection Agency (EPA) risk assessment models, EPA exposure assumptions, as well as to specific chemical warfare agent parameters (e.g., toxicity values). Comparison was made between screening value estimates recalculated with current assumptions and earlier health-based environmental screening levels presented in 1999. The chemical warfare agents evaluated include the G-series and VX nerve agents and the vesicants sulfur mustard (agent HD) and Lewisite (agent L). In addition, key degradation products of these agents were also evaluated. Study findings indicate that the combined effect of updates and/or changes to EPA risk models, EPA default exposure parameters, and certain chemical warfare agent toxicity criteria does not result in significant alteration to the USACHPPM (1999) health-based environmental screening level estimates for the G-series and VX nerve agents or the vesicant agents HD and L. Given that EPA's final position on separate Tier 1 screening levels for indoor and outdoor worker screening assessments has not yet been released as of May 2007, the study authors find that the 1999 screening level estimates (see Table ES.1) are still appropriate and protective for screening residential as well as nonresidential sites. As such, risk management decisions made on the basis of USACHPPM (1999) recommendations do not require reconsideration. While the 1999 HBESL values are appropriate for continued use as general screening criteria, the updated '2007' estimates (presented below) that follow the new EPA protocols currently under development are also

  2. CHRONIC ZINC SCREENING WATER EFFECT RATIO FOR THE H-12 OUTFALL, SAVANNAH RIVER SITE

    SciTech Connect (OSTI)

    Coughlin, D.; Looney, B.; Millings, M.

    2009-01-13

    In response to proposed Zn limits for the NPDES outfall H-12, a Zn screening Water Effects Ratio (WER) study was conducted to determine if a full site-specific WER is warranted. Using standard assumptions for relating the lab results to the stream, the screening WER data were consistent with the proposed Zn limit and suggest that a full WER would result in a similar limit. Addition of a humate amendment to the outfall water reduced Zn toxicity, but the toxicity reduction was relatively small and unlikely to impact proposed Zn limits. The screening WER data indicated that the time and expense required to perform a full WER for Zn is not warranted.

  3. Lignin structural alterations in thermochemical pretreatments with limited delignification

    SciTech Connect (OSTI)

    Pu, Yunqiao; Hu, Fan; Huang, Fang; Ragauskas, Arthur J.

    2015-08-02

    Lignocellulosic biomass has a complex and rigid cell wall structure that makes biomass recalcitrant to biological and chemical degradation. Among the three major structural biopolymers (i.e., cellulose, hemicellulose and lignin) in plant cell walls, lignin is considered the most recalcitrant component and generally plays a negative role in the biochemical conversion of biomass to biofuels. The conversion of biomass to biofuels through a biochemical platform usually requires a pretreatment stage to reduce the recalcitrance. Pretreatment renders compositional and structural changes of biomass with these changes ultimately govern the efficiency of the subsequent enzymatic hydrolysis. Dilute acid, hot water, steam explosion, and ammonia fiber expansion pretreatments are among the leading thermochemical pretreatments with a limited delignification that can reduce biomass recalcitrance. Practical applications of these pretreatment are rapidly developing as illustrated by recent commercial scale cellulosic ethanol plants. While these thermochemical pretreatments generally lead to only a limited delignification and no significant change of lignin content in the pretreated biomass, the lignin transformations that occur during these pretreatments and the roles they play in recalcitrance reduction is an important research aspect. This review highlights recent advances in our understanding of lignin alterations during these limited delignification thermochemical pretreatments, with emphasis on lignin chemical structures, molecular weights, and redistributions in the pretreated biomass.

  4. Lignin structural alterations in thermochemical pretreatments with limited delignification

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pu, Yunqiao; Hu, Fan; Huang, Fang; Ragauskas, Arthur J.

    2015-08-02

    Lignocellulosic biomass has a complex and rigid cell wall structure that makes biomass recalcitrant to biological and chemical degradation. Among the three major structural biopolymers (i.e., cellulose, hemicellulose and lignin) in plant cell walls, lignin is considered the most recalcitrant component and generally plays a negative role in the biochemical conversion of biomass to biofuels. The conversion of biomass to biofuels through a biochemical platform usually requires a pretreatment stage to reduce the recalcitrance. Pretreatment renders compositional and structural changes of biomass with these changes ultimately govern the efficiency of the subsequent enzymatic hydrolysis. Dilute acid, hot water, steam explosion,more » and ammonia fiber expansion pretreatments are among the leading thermochemical pretreatments with a limited delignification that can reduce biomass recalcitrance. Practical applications of these pretreatment are rapidly developing as illustrated by recent commercial scale cellulosic ethanol plants. While these thermochemical pretreatments generally lead to only a limited delignification and no significant change of lignin content in the pretreated biomass, the lignin transformations that occur during these pretreatments and the roles they play in recalcitrance reduction is an important research aspect. This review highlights recent advances in our understanding of lignin alterations during these limited delignification thermochemical pretreatments, with emphasis on lignin chemical structures, molecular weights, and redistributions in the pretreated biomass.« less

  5. The role of screening of the electron-phonon interaction in relaxation of photoexcited electron-hole plasma in semiconductors

    SciTech Connect (OSTI)

    Kumekov, S. E.

    2008-08-15

    The role of screening of the interaction of the electron-hole plasma with optical phonons is analytically evaluated by the example of gallium arsenide.

  6. Agenda from the U.S. Department of Energy's High Throughput Screening of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Storage Materials Workshop on June 26, 2007 | Department of Energy from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Agenda from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Agenda from the U.S. Department of Energy's Hydrogen Storage Meeting held June 26, 2007 in Bethesda, Maryland. ht_agenda.pdf (85.29 KB) More Documents & Publications

  7. Influence of architectural screens on rooftop concentrations due to effluent from short stacks

    SciTech Connect (OSTI)

    Petersen, R.L.; Carter, J.J.; Ratcliff, M.A.

    1999-07-01

    This paper describes the wind tunnel study conducted on behalf of the American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE) to evaluate and quantify the effect of architectural screens on rooftop concentration levels due to effluent from short stacks. An equivalent stack height (ESH) concept is introduced, which is used to develop a stack height reduction (SHR) factor that may be used in conjunction with existing stack design procedures found in the 1997 ASHRAE Handbook--Fundamentals to account for the presence of architectural screens.

  8. Toward Joint Hypothesis-Tests Seismic Event Screening Analysis: Ms|mb and Event Depth

    SciTech Connect (OSTI)

    Anderson, Dale; Selby, Neil

    2012-08-14

    Well established theory can be used to combine single-phenomenology hypothesis tests into a multi-phenomenology event screening hypothesis test (Fisher's and Tippett's tests). Commonly used standard error in Ms:mb event screening hypothesis test is not fully consistent with physical basis. Improved standard error - Better agreement with physical basis, and correctly partitions error to include Model Error as a component of variance, correctly reduces station noise variance through network averaging. For 2009 DPRK test - Commonly used standard error 'rejects' H0 even with better scaling slope ({beta} = 1, Selby et al.), improved standard error 'fails to rejects' H0.

  9. Office of Worker Screening and Compensation Support Workers' Spotlight, July, August, September 2014

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Issue 14 July/August/September 2014 Office of Worker Screening and Compensation Support I N S I D E T H I S I S S U E : Director's Note 1 Awards 1 Museum 2 Trivia 3 Calendar 4 A NOTE FROM THE DIRECTOR By Greg Lewis In the past few months there have been a number of changes that have taken place at DOE Headquarters, which have affected the Office of Worker Screening and Compensation Support, both with our organizational structure and with our staffing. First, the DOE Office of 2014 SYLVIA KIEDING

  10. Automated High Throughput Protein Crystallization Screening at Nanoliter Scale and Protein Structural Study on Lactate Dehydrogenase

    SciTech Connect (OSTI)

    Fenglei Li

    2006-08-09

    The purposes of our research were: (1) To develop an economical, easy to use, automated, high throughput system for large scale protein crystallization screening. (2) To develop a new protein crystallization method with high screening efficiency, low protein consumption and complete compatibility with high throughput screening system. (3) To determine the structure of lactate dehydrogenase complexed with NADH by x-ray protein crystallography to study its inherent structural properties. Firstly, we demonstrated large scale protein crystallization screening can be performed in a high throughput manner with low cost, easy operation. The overall system integrates liquid dispensing, crystallization and detection and serves as a whole solution to protein crystallization screening. The system can dispense protein and multiple different precipitants in nanoliter scale and in parallel. A new detection scheme, native fluorescence, has been developed in this system to form a two-detector system with a visible light detector for detecting protein crystallization screening results. This detection scheme has capability of eliminating common false positives by distinguishing protein crystals from inorganic crystals in a high throughput and non-destructive manner. The entire system from liquid dispensing, crystallization to crystal detection is essentially parallel, high throughput and compatible with automation. The system was successfully demonstrated by lysozyme crystallization screening. Secondly, we developed a new crystallization method with high screening efficiency, low protein consumption and compatibility with automation and high throughput. In this crystallization method, a gas permeable membrane is employed to achieve the gentle evaporation required by protein crystallization. Protein consumption is significantly reduced to nanoliter scale for each condition and thus permits exploring more conditions in a phase diagram for given amount of protein. In addition

  11. Effect of pelleting on the recalcitrance and bioconversion of dilute-acid pretreated corn stover

    SciTech Connect (OSTI)

    Allison E Ray; Amber Hoover; Gary Gresham

    2012-07-01

    Background: Knowledge regarding the performance of densified biomass in biochemical processes is limited. The effects of densification on biochemical conversion are explored here. Methods: Pelleted corn stover samples were generated from bales that were milled to 6.35 mm. Low-solids acid pretreatment and simultaneous saccharification and fermentation were performed to evaluate pretreatment efficacy and ethanol yields achieved for pelleted and ground stover (6.35 mm and 2 mm) samples. Both pelleted and 6.35-mm ground stover were evaluated using a ZipperClave® reactor under high-solids, process-relevant conditions for multiple pretreatment severities (Ro), followed by enzymatic hydrolysis of the washed, pretreated solids. Results: Monomeric xylose yields were significantly higher for pellets (approximately 60%) than for ground formats (approximately 38%). Pellets achieved approximately 84% of theoretical ethanol yield (TEY); ground stover formats had similar profiles, reaching approximately 68% TEY. Pelleting corn stover was not detrimental to pretreatment efficacy for both low- and high-solids conditions, and even enhanced ethanol yields.

  12. Key gene regulating cell wall biosynthesis and recalcitrance in Populus, gene Y

    SciTech Connect (OSTI)

    Chen, Jay; Engle, Nancy; Gunter, Lee E.; Jawdy, Sara; Tschaplinski, Timothy J.; Tuskan, Gerald A.

    2015-12-08

    This disclosure provides methods and transgenic plants for improved production of renewable biofuels and other plant-derived biomaterials by altering the expression and/or activity of Gene Y, an O-acetyltransferase. This disclosure also provides expression vectors containing a nucleic acid (Gene Y) which encodes the polypeptide of SEQ ID NO: 1 and is operably linked to a heterologous promoter.

  13. Evaluating radiographers' diagnostic accuracy in screen-reading mammograms: what constitutes a quality study?

    SciTech Connect (OSTI)

    Debono, Josephine C; Poulos, Ann E

    2015-03-15

    The aim of this study was to first evaluate the quality of studies investigating the diagnostic accuracy of radiographers as mammogram screen-readers and then to develop an adapted tool for determining the quality of screen-reading studies. A literature search was used to identify relevant studies and a quality evaluation tool constructed by combining the criteria for quality of Whiting, Rutjes, Dinnes et al. and Brealey and Westwood. This constructed tool was then applied to the studies and subsequently adapted specifically for use in evaluating quality in studies investigating diagnostic accuracy of screen-readers. Eleven studies were identified and the constructed tool applied to evaluate quality. This evaluation resulted in the identification of quality issues with the studies such as potential for bias, applicability of results, study conduct, reporting of the study and observer characteristics. An assessment of the applicability and relevance of the tool for this area of research resulted in adaptations to the criteria and the development of a tool specifically for evaluating diagnostic accuracy in screen-reading. This tool, with further refinement and rigorous validation can make a significant contribution to promoting well-designed studies in this important area of research and practice.

  14. Observation of finite-wavelength screening in high-energy-density matter

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chapman, D. A.; Vorberger, J.; Fletcher, L. B.; Baggott, R. A.; Divol, L.; Döppner, T.; Falcone, R. W.; Glenzer, S. H.; Gregori, G.; Guymer, T. M.; et al

    2015-04-23

    A key component for the description of charged particle systems is the screening of the Coulomb interaction between charge carriers. First investigated in the 1920s by Debye and Hückel for electrolytes, charge screening is important for determining the structural and transport properties of matter as diverse as astrophysical and laboratory plasmas, nuclear matter such as quark-gluon plasmas, electrons in solids, planetary cores and charged macromolecules. For systems with negligible dynamics, screening is still mostly described using a Debye–Hückel-type approach. Here, we report the novel observation of a significant departure from the Debye–Hückel-type model in high-energy-density matter by probing laser-driven, shock-compressedmore » plastic with high-energy X-rays. We use spectrally resolved X-ray scattering in a geometry that enables direct investigation of the screening cloud, and demonstrate that the observed elastic scattering amplitude is only well described within a more general approach.« less

  15. Screening assessment and requirements for a comprehensive assessment: Volume 1, Draft. Columbia River comprehensive impact assessment

    SciTech Connect (OSTI)

    1997-04-01

    To evaluate the impact to the Columbia River from the Hanford Site-derived contaminants, the U.S. Department of Energy, U.S. Environmental Protection Agency, and Washington State Department of Ecology initiated a study referred to as the Columbia River Comprehensive Impact Assessment (CRCIA). To address concerns about the scope and direction of CRCIA as well as enhance regulator, tribal, stockholder, and public involvement, the CRCIA Management Team was formed in August 1995. The Team agreed to conduct CRCIA using a phased approach. The initial phase, includes two components: 1) a screening assessment to evaluate the potential impact to the river, resulting from current levels of Hanford-derived contaminants in order to support decisions on Interim Remedial Measures, and 2) a definition of the essential work remaining to provide an acceptable comprehensive river impact assessment. The screening assessment is described in Part I of this report. The essential work remaining is Part II of this report. The objective of the screening assessment is to identify areas where the greatest potential exists for adverse effects on humans or the environment. Part I of this report discusses the scope, technical approach, and results of the screening assessment. Part II defines a new paradigm for predecisional participation by those affected by Hanford cleanup decisions.

  16. Observation of finite-wavelength screening in high-energy-density matter

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chapman, D. A.; Vorberger, J.; Fletcher, L. B.; Baggott, R. A.; Divol, L.; Döppner, T.; Falcone, R. W.; Glenzer, S. H.; Gregori, G.; Guymer, T. M.; et al

    2015-04-23

    A key component for the description of charged particle systems is the screening of the Coulomb interaction between charge carriers. First investigated in the 1920s by Debye and Hückel for electrolytes, charge screening is important for determining the structural and transport properties of matter as diverse as astrophysical and laboratory plasmas, nuclear matter such as quark-gluon plasmas, electrons in solids, planetary cores and charged macromolecules. For systems with negligible dynamics, screening is still mostly described using a Debye–Hückel-type approach. Here, we report the novel observation of a significant departure from the Debye–Hückel-type model in high-energy-density matter by probing laser-driven, shock-compressedmore »plastic with high-energy X-rays. We use spectrally resolved X-ray scattering in a geometry that enables direct investigation of the screening cloud, and demonstrate that the observed elastic scattering amplitude is only well described within a more general approach.« less

  17. Observation of finite-wavelength screening in high-energy-density matter

    SciTech Connect (OSTI)

    Chapman, D. A.; Vorberger, J.; Fletcher, L. B.; Baggott, R. A.; Divol, L.; Döppner, T.; Falcone, R. W.; Glenzer, S. H.; Gregori, G.; Guymer, T. M.; Kritcher, A. L.; Landen, O. L.; Ma, T.; Pak, A. E.; Gericke, D. O.

    2015-04-23

    A key component for the description of charged particle systems is the screening of the Coulomb interaction between charge carriers. First investigated in the 1920s by Debye and Hückel for electrolytes, charge screening is important for determining the structural and transport properties of matter as diverse as astrophysical and laboratory plasmas, nuclear matter such as quark-gluon plasmas, electrons in solids, planetary cores and charged macromolecules. For systems with negligible dynamics, screening is still mostly described using a Debye–Hückel-type approach. Here, we report the novel observation of a significant departure from the Debye–Hückel-type model in high-energy-density matter by probing laser-driven, shock-compressed plastic with high-energy X-rays. We use spectrally resolved X-ray scattering in a geometry that enables direct investigation of the screening cloud, and demonstrate that the observed elastic scattering amplitude is only well described within a more general approach.

  18. New Screening System Detects Algae with Increased H2 Production (Fact Sheet), NREL Highlights in Science, NREL (National Renewable Energy Laboratory)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL's high-throughput screen facilitates the selection of novel H 2 -producing algae. Researchers at the National Renewable Energy Laboratory (NREL) have developed a powerful method for screening through million-member algal libraries for strains with increased hydro- gen production. The screen uses H 2 -sensing bacteria that fluoresce when hydrogen is detected and is used as an agar overlay on top of growing algal colonies. The screen was first verified by comparing algal strains that

  19. Accelerated screening methods for determining chemical and thermal stability of refrigerant-lubricant mixtures, Part II: Experimental comparison and verification of methods. Volume 2, In situ conductivity data

    SciTech Connect (OSTI)

    Kauffman, R.

    1995-09-01

    Data are presented for the accelerated screening methods for determining chemical and thermal stability of refrigerant-lubricant mixtures.

  20. Large-Scale Computational Screening of Zeolites for Ethane/Ethene Separation

    SciTech Connect (OSTI)

    Kim, J; Lin, LC; Martin, RL; Swisher, JA; Haranczyk, M; Smit, B

    2012-08-14

    Large-scale computational screening of thirty thousand zeolite structures was conducted to find optimal structures for seperation of ethane/ethene mixtures. Efficient grand canonical Monte Carlo (GCMC) simulations were performed with graphics processing units (GPUs) to obtain pure component adsorption isotherms for both ethane and ethene. We have utilized the ideal adsorbed solution theory (LAST) to obtain the mixture isotherms, which were used to evaluate the performance of each zeolite structure based on its working capacity and selectivity. In our analysis, we have determined that specific arrangements of zeolite framework atoms create sites for the preferential adsorption of ethane over ethene. The majority of optimum separation materials can be identified by utilizing this knowledge and screening structures for the presence of this feature will enable the efficient selection of promising candidate materials for ethane/ethene separation prior to performing molecular simulations.

  1. Effects of dynamic screening on the electrical conductivity of fully ionized, nondegenerate hydrogen plasma

    SciTech Connect (OSTI)

    Redmer, R.A.; Roepke, G.; Morales, F.; Kilimann, K. )

    1990-02-01

    The electrical conductivity of fully ionized, nondegenerate hydrogen plasma is expressed within the Zubarev method by equilibrium correlation functions. Using the Green's function technique, the Lenard--Balescu--Gurnsey collision integral of a generalized Boltzmann equation is derived that accounts for the effects of dynamic screening. Applying the usual random phase approximation, numerical results for the collision integral and the electrical conductivity are compared with the case of static screening ({omega}=0) and the long-wavelength limit ({ital q}{r arrow}0) for the dielectric function {Epsilon}({ital q},{omega}). Effective low-density expansions are given for the collision integrals as well as for the electrical conductivity that are applicable for a wide range of density and temperature.

  2. Control Of Screening Of A Charged Particle In Electrolytic Aqueous Paul Trap

    SciTech Connect (OSTI)

    Park, Jae Hyun; Krstic, Predrag S.

    2011-06-01

    Individual charged particles could be trapped and confined by the combined radio-frequency and DC quadrupole electric field of an aqueous Paul trap. Viscosity of water improves confinement and extends the range of the trap parameters which characterize the stability of the trap. Electrolyte, if present in aqueous solution, may screen the charged particle and thus partially or fully suppress electrophoretic interaction with the applied filed, possibly reducing it to a generally much weaker dielectrophoretic interaction with an induced dipole. Applying molecular dynamics simulation we show that the quadrupole field has a different effect at the electrolyte ions and at much heavier charged particle, effectively eliminating the screening by electrolyte ions and reinstating the electrophoretic confinement.

  3. Subtask 1.11 -- Spectroscopic field screening of hazardous waste and toxic spills. Final report

    SciTech Connect (OSTI)

    Grisanti, A.A.

    1997-10-01

    Techniques for the field characterization of soil contamination due to spillage of hazardous waste or toxic chemicals are time-consuming and expensive. Thus more economical, less time-intensive methods are needed to facilitate rapid field screening of contaminated sites. The overall objective of this project is to study the feasibility of using an evanescent field absorbance sensor Fourier transform infrared spectroscopic sensor coupled with cone penetrometry as a field screening method. The specific objectives of this project are as follows: design an accessory for use with FT-IR that interfaces the spectrometer to a cone penetrometer; characterize the response of the FT-IR accessory to selected hydrocarbons in a laboratory-simulated field environment; and determine the ability of the FT-IR-CPT instrument to measure hydrocarbon contamination in soil by direct comparison with a reference method (e.g., Soxhlet extraction followed by gas chromatography) to quantify hydrocarbons from the same soil.

  4. SU-E-P-03: Implementing a Low Dose Lung Screening CT Program Meeting Regulatory Requirements

    SciTech Connect (OSTI)

    LaFrance, M; Marsh, S; O'Donnell, G

    2014-06-01

    Purpose: To provide information pertaining to IROC Houston QA Center's (RPC) credentialing process for institutions participating in NCI-sponsored clinical trials. Purpose: Provide guidance to the Radiology Departments with the intent of implementing a Low Dose CT Screening Program using different CT Scanners with multiple techniques within the framework of the required state regulations. Method: State Requirements for the purpose of implementing a Low Dose CT Lung Protocol required working with the Radiology and Pulmonary Department in setting up a Low Dose Screening Protocol designed to reduce the radiation burden to the patients enrolled. Radiation dose measurements (CTDIvol) for various CT manufacturers (Siemens16, Siemens 64, Philips 64, and Neusoft128) for three different weight based protocols. All scans were reviewed by the Radiologist. Prior to starting a low dose lung screening protocol, information had to be submitted to the state for approval. Performing a Healing Arts protocol requires extensive information. This not only includes name and address of the applicant but a detailed description of the disease, the x-ray examination and the population to be examined. The unit had to be tested by a qualified expert using the technique charts. The credentials of all the operators, the supervisors and the Radiologists had to be submitted to the state. Results: All the appropriate documentation was sent to the state for review. The measured results between the Low Dose Protocol versus the default Adult Chest Protocol showed that there was a dose reduction of 65% for small (100-150 lb.) patient, 75% for the Medium patient (151-250 lbs.), and a 55% reduction for the Large patient ( over 250 lbs.). Conclusion: Measured results indicated that the Low Dose Protocol indeed lowered the screening patient's radiation dose and the institution was able to submit the protocol to the State's regulators.

  5. INEEL Subsurface Disposal Area CERCLA-based Decision Analysis for Technology Screening and Remedial Alternative Evaluation

    SciTech Connect (OSTI)

    Parnell, G. S.; Kloeber, Jr. J.; Westphal, D; Fung, V.; Richardson, John Grant

    2000-03-01

    A CERCLA-based decision analysis methodology for alternative evaluation and technology screening has been developed for application at the Idaho National Engineering and Environmental Laboratory WAG 7 OU13/14 Subsurface Disposal Area (SDA). Quantitative value functions derived from CERCLA balancing criteria in cooperation with State and Federal regulators are presented. A weighted criteria hierarchy is also summarized that relates individual value function numerical values to an overall score for a specific technology alternative.

  6. Self-powered Gating and Other Improvements for Screening-engineered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Field-effect Photovoltaics - Energy Innovation Portal Solar Photovoltaic Solar Photovoltaic Find More Like This Return to Search Self-powered Gating and Other Improvements for Screening-engineered Field-effect Photovoltaics Field-effect P-N Junctions for Low Cost, High Efficiency Solar Cells and Electronic Devices Lawrence Berkeley National Laboratory Contact LBL About This Technology Publications: PDF Document Publication LBNL Commercial Analysis Report (1,863 KB) Technology Marketing

  7. Guidance Manual for Conducting Screening Level Ecological Risk Assessments at the INEL

    SciTech Connect (OSTI)

    R. L. VanHorn; N. L. Hampton; R. C. Morris

    1995-06-01

    This document presents reference material for conducting screening level ecological risk assessments (SLERAs)for the waste area groups (WAGs) at the Idaho National Engineering Laboratory. Included in this document are discussions of the objectives of and processes for conducting SLERAs. The Environmental Protection Agency ecological risk assessment framework is closely followed. Guidance for site characterization, stressor characterization, ecological effects, pathways of contaminant migration, the conceptual site model, assessment endpoints, measurement endpoints, analysis guidance, and risk characterization are included.

  8. A screening approach for identifying environmental justice issues in environmental impact statements

    SciTech Connect (OSTI)

    Schexnayder, S.S.

    1995-12-01

    Executive Order 12898 and the accompanying memorandum addressed to Federal agency heads, both issued on February 11, 1994, require NEPA processes to incorporate environmental justice. The NEPA processes affected are: (1) public involvement formats, (2) analyses of potential impacts. The Executive Order clearly indicates that research strategies and mitigation measure should be developed with the input of the populations mentioned in the Executive Order, i.e., minority and low-income populations. However, an enhanced public involvement process may not occur because the NEPA activity may have been underway before the Executive Order was issued or because the agency chooses not to change traditional public participation mechanisms. It is also possible that enhanced mechanisms may not effectively elicit involvement. In either case, analysis that considers environmental justice must proceed. These analyses could be highly data-intensive--requiring new or modified methodological approaches-- and time-intensive, particularly if the process elements of the executive order are interpreted broadly, Federal agencies and NEPA project managers already have expressed concern about the potential cost of conducting exhaustive environmental justice related analyses where they may not be warranted. Also, the time and resources required to conduct a full environmental justice analysis is counter to recent trends to streamline the NEPA process. In light of this, a process to screen for indicators of the potential for environmental justice issues has been developed. The method incorporates separate screens for human health impacts, socioeconomic impacts, and social structural impacts. Positive results of any screen indicates the need for full-scale, environmental-justice-related analysis of that category of impact. The screen is intended as a useful tool in implementing environmental justice in environmental impact statements.

  9. Sandia, UCLA develop screening libraries to discover drug targets for viral

    National Nuclear Security Administration (NNSA)

    infections | National Nuclear Security Administration | (NNSA) Sandia, UCLA develop screening libraries to discover drug targets for viral infections Friday, April 22, 2016 - 12:00am From left, Sandia National Laboratories researchers Oscar Negrete, Edwin Saada and Sara Bird check out a CRISPR library preparation. The work is part of the first-ever Cooperative Research and Development Agreement between Sandia and UCLA. As headlines highlight the threat of viruses like Ebola and Zika,

  10. Screen printed silver top electrode for efficient inverted organic solar cells

    SciTech Connect (OSTI)

    Kim, Junwoo; Duraisamy, Navaneethan; Lee, Taik-Min; Kim, Inyoung; Choi, Kyung-Hyun

    2015-10-15

    Highlights: • Screen printing of silver pattern. • X-ray diffraction pattern confirmed the face centered cubic structure of silver. • Uniform surface morphology of silver pattern with sheet resistance of 0.06 Ω/sq. • The power conversion efficiency of fabricated solar cell is found to be 2.58%. - Abstract: The present work is mainly focused on replacement of the vacuum process for top electrode fabrication in organic solar cells. Silver top electrode deposited through solution based screen printing on pre-deposited polymeric thin film. The solution based printing technology provides uniform top electrode without damaging the underlying organic layers. The surface crystallinity and surface morphology of silver top electrode are examined through X-ray diffraction, field-emission scanning electron microscope and atomic force microscope. The purity of silver is examined through X-ray energy dispersive spectroscopy. The top electrode exhibits face centered cubic structure with homogeneous morphology. The sheet resistance of top electrode is found to be 0.06 Ω/sq and an average pattern thickness of ∼15 μm. The power conversion efficiency is 2.58%. Our work demonstrates that the solution based screen printing is a significant role in the replacement of vacuum process for the fabrication of top electrode in organic solar cells.

  11. Volatile organic chemical emissions from carpet cushions: Screening measurements. Final report

    SciTech Connect (OSTI)

    Hodgson, A.T.; Phan, T.A.

    1994-05-01

    The US Consumer Product Safety Commission (CPSC) has received complaints from consumers regarding the occurrence of adverse health effects following the installation of new carpeting (Schachter, 1990). Carpet systems are suspected of emitting chemicals which may be the cause of these complaints, as well as objectionable odors. Carpets themselves have been shown to emit a variety of volatile organic compounds (VOCs). The objective of this study was to screen the representative samples of carpet cushions for emissions of individual VOCS, total VOCs (TVOC), formaldehyde, and, for the two types of polyurethane cushions, isomers of toluene diisocyanate (TDI). The measurements of VOCS, TVOC and formaldehyde were made over six-hour periods using small-volume (4-L) dynamic chambers. Sensitive gas chromatography-mass spectrometry (GC-MS) techniques were used to identify many of the VOCs emitted by the cushion samples and to obtain quantitative estimates of the emission rates of selected compounds. Separate screening measurements were conducted for TDI. The data from the screening measurements were used by the CPSC`s Health Sciences Laboratory to help design and conduct week-long measurements of emission rates of selected compounds.

  12. Site Screening and Technical Guidance for Monitored Natural Attenuation at DOE Sites

    SciTech Connect (OSTI)

    Borns, D.J.; Brady, P.V.; Brady, W.D.; Krupka, K.M.; Spalding, B.P.; Waters, R.D.; Zhang, P.

    1999-03-01

    Site Screening and Technical Guidance for Monitored Natural Attenuation at DOE Sites briefly outlines the biological and geochemical origins of natural attenuation, the tendency for natural processes in soils to mitigate contaminant transport and availability, and the means for relying on monitored natural attenuation (MNA) for remediation of contaminated soils and groundwaters. This report contains a step-by-step guide for (1) screening contaminated soils and groundwaters on the basis of their potential for remediation by natural attenuation and (2) implementing MNA consistent with EPA OSWER Directive 9200.4-17. The screening and implementation procedures are set up as a web-based tool (http://www.sandia.gov/eesector/gs/gc/na/mnahome.html) to assist US Department of Energy (DOE) site environmental managers and their staff and contractors to adhere to EPA guidelines for implementing MNA. This document is intended to support the Decision Maker's Framework Guide and Monitoring Guide both to be issued from DOE EM-40. Further technical advances may cause some of the approach outlined in this document to change over time.

  13. Using the Choquet integral for screening geological CO2 storage sites

    SciTech Connect (OSTI)

    Zhang, Y.

    2011-03-01

    For geological CO{sub 2} storage site selection, it is desirable to reduce the number of candidate sites through a screening process before detailed site characterization is performed. Screening generally involves defining a number of criteria which then need to be evaluated for each site. The importance of each criterion to the final evaluation will generally be different. Weights reflecting the relative importance of these criteria can be provided by experts. To evaluate a site, each criterion must be evaluated and scored, and then aggregated, taking into account the importance of the criteria. We propose the use of the Choquet integral for aggregating the scores. The Choquet integral considers the interactions among criteria, i.e. whether they are independent, complementary to each other, or partially repetitive. We also evaluate the Shapley index, which demonstrates how the importance of a given piece of information may change if it is considered by itself or together with other available information. An illustrative example demonstrates how the Choquet integral properly accounts for the presence of redundancy in two site-evaluation criteria, making the screening process more defensible than the standard weighted-average approach.

  14. Automated assessment of bilateral breast volume asymmetry as a breast cancer biomarker during mammographic screening

    SciTech Connect (OSTI)

    Williams, Alex C; Hitt, Austin N; Voisin, Sophie; Tourassi, Georgia

    2013-01-01

    The biological concept of bilateral symmetry as a marker of developmental stability and good health is well established. Although most individuals deviate slightly from perfect symmetry, humans are essentially considered bilaterally symmetrical. Consequently, increased fluctuating asymmetry of paired structures could be an indicator of disease. There are several published studies linking bilateral breast size asymmetry with increased breast cancer risk. These studies were based on radiologists manual measurements of breast size from mammographic images. We aim to develop a computerized technique to assess fluctuating breast volume asymmetry in screening mammograms and investigate whether it correlates with the presence of breast cancer. Using a large database of screening mammograms with known ground truth we applied automated breast region segmentation and automated breast size measurements in CC and MLO views using three well established methods. All three methods confirmed that indeed patients with breast cancer have statistically significantly higher fluctuating asymmetry of their breast volumes. However, statistically significant difference between patients with cancer and benign lesions was observed only for the MLO views. The study suggests that automated assessment of global bilateral asymmetry could serve as a breast cancer risk biomarker for women undergoing mammographic screening. Such biomarker could be used to alert radiologists or computer-assisted detection (CAD) systems to exercise increased vigilance if higher than normal cancer risk is suspected.

  15. Wiki-based Techno Economic Analysis of a Lignocellulosic Biorefinery...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biofuels Biomass and Biofuels Find More Like This ... The model can be used to estimate the economic impact of various ... the economic, environmental, and energetic ...

  16. CLSF (Center for Lignocellulose Structure and Formation) Homepage

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sixteen teams took up the challenge, and a rich variety of images and poems that evoked the complexities, splendor, and awe of energy science. The Office of Science chose the ...

  17. Process Design and Economics for the Conversion of Lignocellulosic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Hydroprocessing and Product Separation ... 44 3.12 Sustainability Metrics ......The analysis does not take into account any policy ...

  18. Process Design and Economics for the Conversion of Lignocellulosic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... conditioning, product recovery and upgrading ... model, by tracking sustainability metrics for the modeled ... Our analysis does not take into account any policy ...

  19. Process Design and Economics for the Conversion of Lignocellulosic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... The first product from this expanded focus was a ... model by tracking sustainability metrics for the modeled ... Our analysis does not take into account any policy ...

  20. Process Design and Economics for Conversion of Lignocellulosic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... 520,000 of Ethanol Product 4.58 Electricity 0 of ... 3.11 Sustainability Metrics ......without subsidies or policy initiatives, by the year ...

  1. Process Design and Economics for the Conversion of Lignocellulosic...

    Office of Scientific and Technical Information (OSTI)

    Sponsoring Org: USDOE Office of Energy Efficiency and Renewable Energy Bioenergy ... PYROLYSIS; HYDROTREATMENT; BIO-OIL; Bioenergy Word Cloud More Like This Full Text ...

  2. Integrating and Piloting Lignocellulose Biomass Conversion Technology (Presentation)

    SciTech Connect (OSTI)

    Schell, D. J.

    2009-06-15

    Presentation on NREL's integrated biomass conversion capabilities. Presented at the 2009 Advanced Biofuels Workshop in Denver, CO, Cellulosic Ethanol session.

  3. Process for the treatment of lignocellulosic biomass (Patent...

    Office of Scientific and Technical Information (OSTI)

    The monosaccharides are used as animal feeds and energy sources for ethanol production. Authors: Dale, Bruce E. Publication Date: 2014-07-08 OSTI Identifier: 1143633 Report ...

  4. Process for the treatment of lignocellulosic biomass (Patent...

    Office of Scientific and Technical Information (OSTI)

    The monosaccharides are used as animal feeds and energy sources for ethanol production. Authors: Dale, Bruce E. ; Lynd, Lee R. ; Laser, Mark Publication Date: 2013-03-12 OSTI ...

  5. Center for Lignocellulose Structure and Function - Contact Us

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contact Us Daniel Cosgrove, Director Biology Department, Pennsylvania State University Phone: 814-863-3892 Email: dcosgrove@psu.edu Candace Haigler, Associate Director Department of Crop Sciences, North Carolina State University Phone: 540-231-4601 Email: Candace_Haigler@ncsu.edu Laura Ullrich (Gilliland), Manager Office Location: South Frear 304A Mailing address: Mueller 208, Biology The Pennsylvania State University University Park, PA 16802 email: LUG11@psu.edu Telephone: (814) 867-4132 For

  6. Center for Lignocellulose Structure and Function - In the News

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the News Journal of Experimental Botany's Special Issue: "From the Nucleus to the Apoplast: Building the Plant's Cell Wall" (January 2016) features three review articles from our research groups. Daniel Cosgrove reviews the differences between elastic modulus and wall extensibility in the context of cell growth, Charles Anderson addresses progress on pectin synthesis and dynamics research, and Tuo Wang and Mei Hong present how solid-state NMR investigations are contributing to our

  7. Center for Lignocellulose Structure and Function - Open Positions

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Open Positions No open positions currently Penn State North Carolina State UVA Oak Ridge Lab Logo MIT logo URI Logo Department of Energy

  8. Center for Lignocellulose Structure and Function - Our People

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    People Charles Anderson Charles T. Anderson Department of Biology, Pennsylvania State University Phone: 814-863-4736 E-mail: cta3@psu.edu Research Website My research group studies the synthesis, modification, and degradation of plant cell walls, focusing on wall polymer network rearrangements during cell growth and on functional interactions between pectins and cellulose. Dan Cosgrove Daniel Cosgrove Biology Department, Pennsylvania State University Phone: 814-863-3892 Email: dcosgrove@psu.edu

  9. Caloramator sp. Tolerance of Pretreatment Inhibitors from Lignocellulosics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Calling All Fuel Cells Calling All Fuel Cells December 7, 2012 - 4:31pm Addthis Altergy had more than 60 fuel cells in the immediate Hurricane Sandy disaster area that acted as backup power for cell phone towers. | Photo courtesy of Altergy. Altergy had more than 60 fuel cells in the immediate Hurricane Sandy disaster area that acted as backup power for cell phone towers. | Photo courtesy of Altergy. Sunita Satyapal Director, Fuel Cell Technologies Office What is a fuel cell? A fuel cell is a

  10. Center for Lignocellulose Structure and Function - Upcoming Events

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Upcoming Events No events currently listed - please check back

  11. Conversion of Lignocellulosic Feedstocks to Aromatic Fuels and...

    Broader source: Energy.gov (indexed) [DOE]

    ... 43.33 38.7 Nitrogen <0.5 <0.5 <0.5 Sulfur <0.05 0.065 <0.05 Chloride 0.0361 0.315 0.0266 Woody Biomass Advantages -CHNO Analysis & Inorganic Ash Concentration -Biomass effect ...

  12. Process Design and Economics for the Conversion of Lignocellulosic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... This preliminary analysis suggests that this fuel meets the EISA RFS cellulosic biofuel ... Standard D4814 - 13a. "Standard Specification for Automotive Spark-Ignition Engine Fuel." ...

  13. Lignocellulosic Biomass to Ethanol Process Design and Economics...

    Broader source: Energy.gov (indexed) [DOE]

    ... An instrument air dryer and a surge tank were designed to provide clean dry air at a ... ways, to provide clean water to the boiler feed water make-up system and the scrubber. ...

  14. High-throughput prediction of Acacia and eucalypt lignin syringyl/guaiacyl content using FT-Raman spectroscopy and partial least squares modeling

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lupoi, Jason S.; Healey, Adam; Singh, Seema; Sykes, Robert; Davis, Mark; Lee, David J.; Shepherd, Merv; Simmons, Blake A.; Henry, Robert J.

    2015-01-16

    High-throughput techniques are necessary to efficiently screen potential lignocellulosic feedstocks for the production of renewable fuels, chemicals, and bio-based materials, thereby reducing experimental time and expense while supplanting tedious, destructive methods. The ratio of lignin syringyl (S) to guaiacyl (G) monomers has been routinely quantified as a way to probe biomass recalcitrance. Mid-infrared and Raman spectroscopy have been demonstrated to produce robust partial least squares models for the prediction of lignin S/G ratios in a diverse group of Acacia and eucalypt trees. The most accurate Raman model has now been used to predict the S/G ratio from 269 unknown Acaciamore » and eucalypt feedstocks. This study demonstrates the application of a partial least squares model composed of Raman spectral data and lignin S/G ratios measured using pyrolysis/molecular beam mass spectrometry (pyMBMS) for the prediction of S/G ratios in an unknown data set. The predicted S/G ratios calculated by the model were averaged according to plant species, and the means were not found to differ from the pyMBMS ratios when evaluating the mean values of each method within the 95 % confidence interval. Pairwise comparisons within each data set were employed to assess statistical differences between each biomass species. While some pairwise appraisals failed to differentiate between species, Acacias, in both data sets, clearly display significant differences in their S/G composition which distinguish them from eucalypts. In conclusion, this research shows the power of using Raman spectroscopy to supplant tedious, destructive methods for the evaluation of the lignin S/G ratio of diverse plant biomass materials.« less

  15. High-throughput prediction of Acacia and eucalypt lignin syringyl/guaiacyl content using FT-Raman spectroscopy and partial least squares modeling

    SciTech Connect (OSTI)

    Lupoi, Jason S.; Healey, Adam; Singh, Seema; Sykes, Robert; Davis, Mark; Lee, David J.; Shepherd, Merv; Simmons, Blake A.; Henry, Robert J.

    2015-01-16

    High-throughput techniques are necessary to efficiently screen potential lignocellulosic feedstocks for the production of renewable fuels, chemicals, and bio-based materials, thereby reducing experimental time and expense while supplanting tedious, destructive methods. The ratio of lignin syringyl (S) to guaiacyl (G) monomers has been routinely quantified as a way to probe biomass recalcitrance. Mid-infrared and Raman spectroscopy have been demonstrated to produce robust partial least squares models for the prediction of lignin S/G ratios in a diverse group of Acacia and eucalypt trees. The most accurate Raman model has now been used to predict the S/G ratio from 269 unknown Acacia and eucalypt feedstocks. This study demonstrates the application of a partial least squares model composed of Raman spectral data and lignin S/G ratios measured using pyrolysis/molecular beam mass spectrometry (pyMBMS) for the prediction of S/G ratios in an unknown data set. The predicted S/G ratios calculated by the model were averaged according to plant species, and the means were not found to differ from the pyMBMS ratios when evaluating the mean values of each method within the 95 % confidence interval. Pairwise comparisons within each data set were employed to assess statistical differences between each biomass species. While some pairwise appraisals failed to differentiate between species, Acacias, in both data sets, clearly display significant differences in their S/G composition which distinguish them from eucalypts. In conclusion, this research shows the power of using Raman spectroscopy to supplant tedious, destructive methods for the evaluation of the lignin S/G ratio of diverse plant biomass materials.

  16. Environmental screening tools for assessment of infrastructure plans based on biodiversity preservation and global warming (PEIT, Spain)

    SciTech Connect (OSTI)

    Garcia-Montero, Luis G.

    2010-04-15

    Most Strategic Environmental Assessment (SEA) research has been concerned with SEA as a procedure, and there have been relatively few developments and tests of analytical methodologies. The first stage of the SEA is the 'screening', which is the process whereby a decision is taken on whether or not SEA is required for a particular programme or plan. The effectiveness of screening and SEA procedures will depend on how well the assessment fits into the planning from the early stages of the decision-making process. However, it is difficult to prepare the environmental screening for an infrastructure plan involving a whole country. To be useful, such methodologies must be fast and simple. We have developed two screening tools which would make it possible to estimate promptly the overall impact an infrastructure plan might have on biodiversity and global warming for a whole country, in order to generate planning alternatives, and to determine whether or not SEA is required for a particular infrastructure plan.

  17. Walla Walla River Basin Fish Screen Evaluations, 2003: Nursery Bridge Fishway and Garden City-Lowden II

    SciTech Connect (OSTI)

    Vucelick, Jessica A.; McMichael, Geoffrey A.

    2003-11-01

    The Pacific Northwest National Laboratory (PNNL) evaluated the fish screens at the Nursery Bridge Fishway and at the newly constructed Garden City-Lowden II site west of Walla Walla, Washington in the Walla Walla River Basin during the spring and summer of 2003. Both fish screen facilities were examined to determine if they were being effectively operated and maintained to provide for safe fish passage. At the Nursery Bridge Fishway, the screens were evaluated specifically to determine whether the louvers that aid in controlling water flow from behind the screens could be adjusted so that the screens would meet fish protection criteria. Data were collected to determine whether velocities in front of the screens and in the bypasses met current National Oceanic and Atmospheric Administration Fisheries ((NOAA Fisheries), formerly National Marine Fisheries Service (NMFS)) criteria to promote safe and timely fish passage before and after changing the louver settings. Rock weirs downstream of the dam were also evaluated to determine whether they might impede upstream migration of juvenile salmonids during low flow conditions. At the Garden City-Lowden II site, data were collected to establish a baseline for operating conditions and to determine whether any changes in the baffle settings were needed. Based on the results of our studies in 2003, we concluded: Nursery Bridge Site: (1) 68% of the initial velocity measurements on the west screen exceeded the NOAA Fisheries criteria of 0.4 ft/s for approach velocity; (2) A simple adjustment of the existing louvers was not sufficient to fix the problem; (3) The sediment and debris load in the river upstream of the screens exceeded the design criteria for the site, which had frequent breakdowns in the screen cleaning systems; and (4) The rock weirs downstream of the dam would not be expected to impede upstream movement of juvenile fish during low flow conditions. Garden City-Lowden II: (1) The flat inclined-plate screen design

  18. Walla Walla River Basin Fish Screen Evaluations; Nursery Bridge Fishway and Garden City/Lowden II Sites, 2003 Technical Report.

    SciTech Connect (OSTI)

    Vucelick, J.; McMichael, G.

    2003-11-01

    Pacific Northwest National Laboratory evaluated the fish screens at the Nursery Bridge Fishway and the newly constructed Garden City/Lowden II site west of Walla Walla, Washington, in the Walla Walla River Basin during spring and summer 2003. Both fish screen facilities were examined to determine if they were being effectively operated and maintained to provide for safe fish passage. At the Nursery Bridge Fishway, the screens were evaluated specifically to determine whether the louvers that aid in controlling water flow from behind the screens could be adjusted so that the screens would meet fish protection criteria. Data were collected to determine whether velocities in front of the screens and in the bypasses met current National Oceanic and Atmospheric Administration Fisheries (NOAA Fisheries) (formerly National Marine Fisheries Service (NMFS)) criteria to promote safe and timely fish passage before and after changing the louver settings. Rock weirs downstream of the dam were also evaluated to determine whether they might impede upstream migration of juvenile salmonids during low flow conditions. At the Garden City/Lowden II site, data were collected to establish a baseline for operating conditions and to determine whether any changes in the baffle settings were needed.

  19. Use of external metabolizing systems when testing for endocrine disruption in the T-screen assay

    SciTech Connect (OSTI)

    Taxvig, Camilla Olesen, Pelle Thonning; Nellemann, Christine

    2011-02-01

    Although, it is well-established that information on the metabolism of a substance is important in the evaluation of its toxic potential, there is limited experience with incorporating metabolic aspects into in vitro tests for endocrine disrupters. The aim of the current study was a) to study different in vitro systems for biotransformation of ten known endocrine disrupting chemicals (EDs): five azole fungicides, three parabens and 2 phthalates, b) to determine possible changes in the ability of the EDs to bind and activate the thyroid receptor (TR) in the in vitro T-screen assay after biotransformation and c) to investigate the endogenous metabolic capacity of the GH3 cells, the cell line used in the T-screen assay, which is a proliferation assay used for the in vitro detection of agonistic and antagonistic properties of compounds at the level of the TR. The two in vitro metabolizing systems tested the human liver S9 mix and the PCB-induced rat microsomes gave an almost complete metabolic transformation of the tested parabens and phthalates. No marked difference the effects in the T-screen assay was observed between the parent compounds and the effects of the tested metabolic extracts. The GH3 cells themselves significantly metabolized the two tested phthalates dimethyl phthalate (DMP) and diethyl phthalate (DEP). Overall the results and qualitative data from the current study show that an in vitro metabolizing system using liver S9 or microsomes could be a convenient method for the incorporation of metabolic and toxicokinetic aspects into in vitro testing for endocrine disrupting effects.

  20. Toxicological benchmarks for screening potential contaminants of concern for effects on aquatic biota: 1994 Revision

    SciTech Connect (OSTI)

    Suter, G.W. II; Mabrey, J.B.

    1994-07-01

    This report presents potential screening benchmarks for protection of aquatic life from contaminants in water. Because there is no guidance for screening benchmarks, a set of alternative benchmarks is presented herein. The alternative benchmarks are based on different conceptual approaches to estimating concentrations causing significant effects. For the upper screening benchmark, there are the acute National Ambient Water Quality Criteria (NAWQC) and the Secondary Acute Values (SAV). The SAV concentrations are values estimated with 80% confidence not to exceed the unknown acute NAWQC for those chemicals with no NAWQC. The alternative chronic benchmarks are the chronic NAWQC, the Secondary Chronic Value (SCV), the lowest chronic values for fish and daphnids from chronic toxicity tests, the estimated EC20 for a sensitive species, and the concentration estimated to cause a 20% reduction in the recruit abundance of largemouth bass. It is recommended that ambient chemical concentrations be compared to all of these benchmarks. If NAWQC are exceeded, the chemicals must be contaminants of concern because the NAWQC are applicable or relevant and appropriate requirements (ARARs). If NAWQC are not exceeded, but other benchmarks are, contaminants should be selected on the basis of the number of benchmarks exceeded and the conservatism of the particular benchmark values, as discussed in the text. To the extent that toxicity data are available, this report presents the alternative benchmarks for chemicals that have been detected on the Oak Ridge Reservation. It also presents the data used to calculate benchmarks and the sources of the data. It compares the benchmarks and discusses their relative conservatism and utility.

  1. Comparative performance of modern digital mammography systems in a large breast screening program

    SciTech Connect (OSTI)

    Yaffe, Martin J. Bloomquist, Aili K.; Hunter, David M.; Mawdsley, Gordon E.; Chiarelli, Anna M.; Muradali, Derek; Mainprize, James G.

    2013-12-15

    Purpose: To compare physical measures pertaining to image quality among digital mammography systems utilized in a large breast screening program. To examine qualitatively differences in these measures and differences in clinical cancer detection rates between CR and DR among sites within that program. Methods: As part of the routine quality assurance program for screening, field measurements are made of several variables considered to correlate with the diagnostic quality of medical images including: modulation transfer function, noise equivalent quanta, d? (an index of lesion detectability) and air kerma to allow estimation of mean glandular dose. In addition, images of the mammography accreditation phantom are evaluated. Results: It was found that overall there were marked differences between the performance measures of DR and CR mammography systems. In particular, the modulation transfer functions obtained with the DR systems were found to be higher, even for larger detector element sizes. Similarly, the noise equivalent quanta, d?, and the phantom scores were higher, while the failure rates associated with low signal-to-noise ratio and high dose were lower with DR. These results were consistent with previous findings in the authors program that the breast cancer detection rates at sites employing CR technology were, on average, 30.6% lower than those that used DR mammography. Conclusions: While the clinical study was not large enough to allow a statistically powered system-by-system assessment of cancer detection accuracy, the physical measures expressing spatial resolution, and signal-to-noise ratio are consistent with the published finding that sites employing CR systems had lower cancer detection rates than those using DR systems for screening mammography.

  2. Low-Dose Chest Computed Tomography for Lung Cancer Screening Among Hodgkin Lymphoma Survivors: A Cost-Effectiveness Analysis

    SciTech Connect (OSTI)

    Wattson, Daniel A.; Hunink, M.G. Myriam; DiPiro, Pamela J.; Das, Prajnan; Hodgson, David C.; Mauch, Peter M.; Ng, Andrea K.

    2014-10-01

    Purpose: Hodgkin lymphoma (HL) survivors face an increased risk of treatment-related lung cancer. Screening with low-dose computed tomography (LDCT) may allow detection of early stage, resectable cancers. We developed a Markov decision-analytic and cost-effectiveness model to estimate the merits of annual LDCT screening among HL survivors. Methods and Materials: Population databases and HL-specific literature informed key model parameters, including lung cancer rates and stage distribution, cause-specific survival estimates, and utilities. Relative risks accounted for radiation therapy (RT) technique, smoking status (>10 pack-years or current smokers vs not), age at HL diagnosis, time from HL treatment, and excess radiation from LDCTs. LDCT assumptions, including expected stage-shift, false-positive rates, and likely additional workup were derived from the National Lung Screening Trial and preliminary results from an internal phase 2 protocol that performed annual LDCTs in 53 HL survivors. We assumed a 3% discount rate and a willingness-to-pay (WTP) threshold of $50,000 per quality-adjusted life year (QALY). Results: Annual LDCT screening was cost effective for all smokers. A male smoker treated with mantle RT at age 25 achieved maximum QALYs by initiating screening 12 years post-HL, with a life expectancy benefit of 2.1 months and an incremental cost of $34,841/QALY. Among nonsmokers, annual screening produced a QALY benefit in some cases, but the incremental cost was not below the WTP threshold for any patient subsets. As age at HL diagnosis increased, earlier initiation of screening improved outcomes. Sensitivity analyses revealed that the model was most sensitive to the lung cancer incidence and mortality rates and expected stage-shift from screening. Conclusions: HL survivors are an important high-risk population that may benefit from screening, especially those treated in the past with large radiation fields including mantle or involved-field RT. Screening

  3. Combinatorial Screening of Advanced Scintillators for High Resolution X-ray Detectors

    SciTech Connect (OSTI)

    Cheng, Shifan; Tao, Dejie; Lynch, Michael; Yuan, Xianglong; Li, Yiqun

    2008-05-12

    The lack of efficient scintillators is a major problem for developing powerful x-ray detectors that are widely used in homeland security, industrial and scientific research. Intematix has developed and applied a high throughput screening process and corresponding crystal growth technology to significantly speed up the discovery process for new efficient scintillators. As a result, Intematix has invented and fabricated three new scintillators both in powder and bulk forms, which possess promising properties such as better radiation hardness and better matching for silicon diode.

  4. High-stringency screening of target-binding partners using a microfluidic device

    DOE Patents [OSTI]

    Soh, Hyongsok; Lou, Xinhui; Lagally, Eric

    2015-12-01

    The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.

  5. Ohio Envhnmental Protection ~geacy PRS 322 Dayton Unit III Soil screening Result0 Report

    Office of Legacy Management (LM)

    Ohio Envhnmental Protection ~geacy PRS 322 Dayton Unit III Soil screening Result0 Report hbruaty, 1998 Ewcutive !hmmaiy PRS 322 (Dayton Unit III) is one of over 400 PRs' s (Potcnri4 &lease Sires) included in IIIC DOE Mmiamisburg Environment4 Managema Project (MEMP) Mound clesiu~p dceisioa-a suatcgy known aa "Mound 2000". PRS 333 is loeared ia rbe Cjry of Dayron apd is owned and occupied by the Dqmn Board of Education history of polonium-2 10 The site has a processing rbar occwed

  6. Direct laser immobilization of photosynthetic material on screen printed electrodes for amperometric biosensor

    SciTech Connect (OSTI)

    Boutopoulos, Christos; Zergioti, Ioanna; Touloupakis, Eleftherios; Pezzotti, Ittalo; Giardi, Maria Teresa

    2011-02-28

    This letter demonstrates the direct laser printing of photosynthetic material onto low cost nonfunctionalized screen printed electrodes for the fabrication of photosynthesis-based amperometric biosensors. The high kinetic energy of the transferred material induces direct immobilization of the thylakoids onto the electrodes without the use of linkers. This type of immobilization is able to establish efficient electrochemical contact between proteins and electrode, stabilizing the photosynthetic biomolecule and transporting electrons to the solid state device with high efficiency. The functionality of the laser printed biosensors was evaluated by the detection of a common herbicide such as Linuron.

  7. Electroanalytical applications of screen-printable surfactant-induced sol-gel graphite composites

    DOE Patents [OSTI]

    Guadalupe, Ana R.; Guo, Yizhu

    2001-05-15

    A process for preparing sol-gel graphite composite electrodes is presented. This process preferably uses the surfactant bis(2-ethylhexyl) sulfosuccinate (AOT) and eliminates the need for a cosolvent, an acidic catalyst, a cellulose binder and a thermal curing step from prior art processes. Fabrication of screen-printed electrodes by this process provides a simple approach for electroanalytical applications in aqueous and nonaqueous solvents. Examples of applications for such composite electrodes produced from this process include biochemical sensors such as disposable, single-use glucose sensors and ligand modified composite sensors for metal ion sensitive sensors.

  8. Mesoscopic Kondo Screening Effect in a Single-Electron Transistor Embedded in a Metallic Ring

    SciTech Connect (OSTI)

    Hu, Hui; Zhang, Guang-Ming; Yu, Lu

    2001-06-11

    We study the Kondo screening effect generated by a single-electron transistor or quantum dot embedded in a small metallic ring. When the ring circumference L becomes comparable to the fundamental length scale {xi}{sup 0}{sub K}={Dirac_h}{upsilon}{sub F}/ T{sup 0}{sub K} associated with the bulk Kondo temperature, the Kondo resonance is strongly affected, depending on the total number of electrons (mod4) and magnetic flux threading the ring. The resulting Kondo-assisted persistent currents are also calculated in both Kondo and mixed-valence regimes, and the maximum values are found in the crossover region.

  9. Screening Risk Assessment for Possible Radionuclides in the Amchitka Marine Environment

    SciTech Connect (OSTI)

    NNSA /NV

    2002-10-31

    As part of its environmental stewardship program the U.S. Department of Energy (DOE) is reevaluating three sites where underground nuclear tests were conducted in the deep subsurface of Amchitka Island, Alaska. The tests (i.e., Long Shot, Milrow, and Cannikin) were conducted in 1965, 1969, and 1971, respectively. Extensive investigations were conducted on these tests and their effect on the environment. Evaluations at the time of testing indicated limited release of radionuclides and absence of risk related to the testing; however, these are being reevaluated under the current DOE environmental stewardship program. A screening risk assessment of potential radionuclide release into the marine environment is an important part of this reevaluation. The risk assessment is one of three interrelated activities: a groundwater model and this screening risk assessment, both of which guide the decisions in the third activity, the site closure plan. Thus, the overall objective of the work is to understand, and subsequently manage, any risk to humans and the environment through a closure and long-term stewardship plan. The objective of this screening risk assessment is to predict whether possible releases of radionuclides at the ocean floor would represent potential risks to Native Alaskans by consumption of marine subsistence species. In addition, risks were predicted for consumers of commercial catches of marine organisms. These risks were calculated beginning with estimates of possible radionuclide release at the seafloor (from a groundwater modeling study), into the seawater, through possible uptake by marine organisms, and finally possible consumption by humans. The risk assessment model has 11 elements, progressing from potential release at the seafloor through water and food chains to human intake. Data for each of these elements were systematically found and synthesized from many sources, and represent the best available knowledge. Whenever precise data were lacking

  10. Office of Worker Screening and Compensation Support Workers' Spotlight Issue 14 October, November, December 2014

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Issue 15 October/November/December 2014 Office of Worker Screening and Compensation Support I N S I D E T H I S I S S U E : Director's Note 1 Quilt 1 NDR 2 JOTG Town Hall 2 UI Recognition 3 SERT 4 Calendar 4 A NOTE FROM THE DIRECTOR By Greg Lewis The late summer and early fall have been an extremely busy time for AU-14, filled with a number of events and projects. In August, we attended Joint Outreach Task Group (JOTG) events in Spokane and Richland, WA, as well as an annual meeting of the

  11. General guidelines for medically screening mixed population groups potentially exposed to nerve or vesicant agents

    SciTech Connect (OSTI)

    Watson, A.P.; Munro, N.B.; Sidell, F.R.; Leffingwell, S.S.

    1992-01-01

    A number of state and local planners have requested guidance on screening protocols and have expressed interest in sampling body fluids from exposed or potentially exposed individuals as a means of estimating agent dose. These guidelines have been developed to provide a clear statement that could be used by state and local emergency response personnel in the event of a nerve or vesicant agent incident resulting in off-post contamination; maximum protection from harm is the goal. The assumption is that any population group so exposed would be heterogeneous for age, gender, reproductive status, and state of health.

  12. Screening study of mixed transition-metal oxides for use as cathodes in thermal batteries

    SciTech Connect (OSTI)

    Guidotti, R.A.; Reinhardt, F.W.

    1996-05-01

    Over 100 candidates were examined, including commercial materials and many that were synthesized in house. The mixed oxides were based on Ti, V, Nb, Cr, Mo, W, Mn, Fe, Co, Ni, and Cu doped with other transition metals. A number of individual (single-metal) oxides were included for comparison. The candidates were tested in single cells with Li(Si) anodes and separators based on LiCl-KCl eutectic. Screening was done under constant-current conditions at current densities of 125 me/cm{sup 2} and, to a lesser extent, 50 me/cm{sup 2} at 500 C. Relative performance and limitations of the oxide cathodes are discussed.

  13. Determination of saccharides and ethanol from biomass conversion using Raman spectroscopy: Effects of pretreatment and enzyme composition

    SciTech Connect (OSTI)

    Shih, Chien-Ju

    2010-05-16

    bioethanol from biomass, has grown significantly in the past decade due to the high demand and rising costs of fossil fuels. More than 3 percent of the energy consumption in the U.S. is derived from renewable biomass, mostly through industrial heat and steam production by the pulp and paper industry, and electricity generation from municipal solid waste (MSW) and forest industry residues. The utilization of food-based biomass to make fuels has been widely criticized because it may increase food shortages throughout the world and raise the cost of food. Thus, nonfood-based and plentiful lignocellulosic feedstocks, such as corn stover, perennial grass, bagasse, sorghum, wheat/rice straw, herbaceous and woody crops, have great potential to be new bio-renewable sources for energy production. Given that many varieties of biomass are available, there is need for a rapid, simple, high-throughput method to screen the conversion of many plant varieties. The most suitable species for each geographic region must be determined, as well as the optimal stage of harvest, impacts of environmental conditions (temperature, soil, pH, etc.). Various genetically modified plants should be studied in order to establish the desired biomass in bioethanol production. The main screening challenge, however, is the complexity of plant cell wall structures that make reliable and sensitive analysis difficult. To date, one of the most popular methods to produce lignocellulosic ethanol is to perform enzymatic hydrolysis followed by fermentation of the hydrolysate with yeast. There are several vital needs related to the field of chemistry that have been suggested as primary research foci needed to effectively improve lignocellulosic ethanol production. These topics include overcoming the recalcitrance of cellulosic biomass, the pervasiveness of pretreatment, advanced biological processing and better feedstocks. In this thesis, a novel approach using Raman spectroscopy has been developed to address important

  14. NMR relaxation and exchange in metal-organic frameworks for surface area screening

    SciTech Connect (OSTI)

    Chen, JJ; Mason, JA; Bloch, ED; Gygi, D; Long, JR; Reimer, JA

    2015-03-15

    We describe a robust screening technique that correlates the surface area of metal organic frameworks to the proton T-2 relaxation behavior of imbibed solvent at low field (13 MHz). In frameworks with small pore sizes (<1 nm) or strong solvent-framework interactions, diffusional exchange between the pore-confined and inter-particle solvent populations remains slow compared to the T-2 of the pore-confined solvent, allowing for a direct porosity analysis of the T-2 spectrum obtained from Laplace inversions. Increases in framework pore-size (>1 nm) lead to corresponding increases in the rate of solvent exchange, as confirmed by T-2 relaxation exchange (REXSY) experiments; increases in the pore size also increases the T-2 of the pore-confined solvent. The combination of these two effects results in comparable rates of relaxation and exchange, which precludes the direct analysis of Laplace inversions. Thus, two- and three-site kinetics models were applied to extract porosity from relaxation decays, thereby improving the utility of the porosity screening tool. (C) 2014 Elsevier Inc. All rights reserved.

  15. In silico screening of metal–organic frameworks in separation applications

    SciTech Connect (OSTI)

    Krishna, Rajamani; van Baten, Jasper M.

    2011-05-03

    Porous materials such as metal–organic frameworks (MOFs) and zeolitic imidazolate frameworks (ZIFs) offer considerable potential for separating a variety of mixtures such as those relevant for CO₂ capture (CO₂/H₂, CO₂/CH₄, CO₂/N₂), CH₄/H₂, alkanes/alkenes, and hydrocarbon isomers. There are basically two different separation technologies that can be employed: (1) a pressure swing adsorption (PSA) unit with a fixed bed of adsorbent particles, and (2) a membrane device, wherein the mixture is allowed to permeate through a micro-porous crystalline layer. In view of the vast number of MOFs, and ZIFs that have been synthesized there is a need for a systematic screening of potential candidates for any given separation task. Also of importance is to investigate how MOFs and ZIFs stack up against the more traditional zeolites such as NaX and NaY with regard to their separation characteristics. This perspective highlights the potency of molecular simulations in determining the choice of the best MOF or ZIF for a given separation task. A variety of metrics that quantify the separation performance, such as adsorption selectivity, working capacity, diffusion selectivity, and membrane permeability, are determined from a combination of Configurational-Bias Monte Carlo (CBMC) and Molecular Dynamics (MD) simulations. The practical utility of the suggested screening methodology is demonstrated by comparison with available experimental data.

  16. Dust particle charge screening in the dry-air plasma produced by an external ionization source

    SciTech Connect (OSTI)

    Derbenev, I. N.; Filippov, A. V.

    2015-08-15

    The ionic composition of the plasma produced by an external ionization source in dry air at atmospheric pressure and room temperature and the screening of the electric field of a dust particle in such a plasma have been investigated. The point sink model based on the diffusion-drift approximation has been used to solve the screening problem. We have established that the main species of ions in the plasma under consideration are O{sub 4}{sup +}, O{sub 2}{sup -}, and O{sub 4}{sup -} and that the dust particle potential distribution is described by a superposition of four exponentials with four different constants. We show that the first constant coincides with the inverse Debye length, the second is described by the inverse ambipolar diffusion length of the positive and negative plasma components in the characteristic time of their recombination, the third is determined by the conversion of negative ions, and the fourth is determined by the attachment and recombination of electrons and diatomic ions.

  17. Cost estimates for the Uranium-in-Soils Integrated Demonstration field screening technologies

    SciTech Connect (OSTI)

    Douthat, D.M.; Armstrong, A.Q.; Ladd, B.

    1993-09-01

    The objective of this document is to describe the work conducted by the ORNL Performance Assessment Group members responsible for developing the cost analysis reports for the uranium-in-soils Integrated Demonstration (ID). The following information is provided in this report: (1) an explanation of the cost input questionnaires, which were sent to the developers of the field screening technologies and used by the cost estimator to acquire information and develop the cost estimates, (2) a description of the computer software package chosen to create the cost estimates, as well as why it was chosen, (3) a description of how the Uranium-in-Soils ID project is broken down structurally in terms of a work breakdown structure (WBS) for the cost estimates, (4) an explanation of the assumptions made by the cost estimator in developing the cost estimates, (5) a summary of the expected costs for each field screening technology, and (6) an explanation of how the cost analysis reports for a scenario evaluation (provided in the cost input questionnaires) were derived, as well as a summary of the scenario evaluation costs for each technology.

  18. Screening method for selecting semiconductor substrates having defects below a predetermined level in an oxide layer

    DOE Patents [OSTI]

    Warren, W.L.; Vanheusden, K.J.R.; Schwank, J.R.; Fleetwood, D.M.; Shaneyfelt, M.R.; Winokur, P.S.; Devine, R.A.B.

    1998-07-28

    A method is disclosed for screening or qualifying semiconductor substrates for integrated circuit fabrication. The method comprises the steps of annealing at least one semiconductor substrate at a first temperature in a defect-activating ambient (e.g. hydrogen, forming gas, or ammonia) for sufficient time for activating any defects within on oxide layer of the substrate; measuring a defect-revealing electrical characteristic of at least a portion of the oxide layer for determining a quantity of activated defects therein; and selecting substrates for which the quantity of activated defects is below a predetermined level. The defect-revealing electrical characteristic may be a capacitance-versus voltage (C-V) characteristic or a current-versus-voltage (I-V) characteristic that is dependent on an electrical charge in the oxide layer generated by the activated defects. Embodiments of the present invention may be applied for screening any type of semiconductor substrate or wafer having an oxide layer formed thereon or therein. This includes silicon-on-insulator substrates formed by a separation by the implantation of oxygen (SIMOX) process or the bond and etch back silicon-on-insulator (BESOI) process, as well as silicon substrates having a thermal oxide layer or a deposited oxide layer. 5 figs.

  19. Plasma nitriding monitoring reactor: A model reactor for studying plasma nitriding processes using an active screen

    SciTech Connect (OSTI)

    Hamann, S. Röpcke, J.; Börner, K.; Burlacov, I.; Spies, H.-J.; Strämke, M.; Strämke, S.

    2015-12-15

    A laboratory scale plasma nitriding monitoring reactor (PLANIMOR) has been designed to study the basics of active screen plasma nitriding (ASPN) processes. PLANIMOR consists of a tube reactor vessel, made of borosilicate glass, enabling optical emission spectroscopy (OES) and infrared absorption spectroscopy. The linear setup of the electrode system of the reactor has the advantages to apply the diagnostic approaches on each part of the plasma process, separately. Furthermore, possible changes of the electrical field and of the heat generation, as they could appear in down-scaled cylindrical ASPN reactors, are avoided. PLANIMOR has been used for the nitriding of steel samples, achieving similar results as in an industrial scale ASPN reactor. A compact spectrometer using an external cavity quantum cascade laser combined with an optical multi-pass cell has been applied for the detection of molecular reaction products. This allowed the determination of the concentrations of four stable molecular species (CH{sub 4}, C{sub 2}H{sub 2}, HCN, and NH{sub 3}). With the help of OES, the rotational temperature of the screen plasma could be determined.

  20. Identification and Prioritization of Analysis Cases for Marine and Hydrokinetic Energy Risk Screening

    SciTech Connect (OSTI)

    Anderson, Richard M.; Unwin, Stephen D.; Van Cleve, Frances B.

    2010-06-16

    In this report we describe the development of the Environmental Risk Evaluation System (ERES), a risk-informed analytical process for estimating the environmental risks associated with the construction and operation of marine and hydrokinetic energy generation projects. The development process consists of two main phases of analysis. In the first phase, preliminary risk analyses will take the form of screening studies in which key environmental impacts and the uncertainties that create risk are identified, leading to a better-focused characterization of the relevant environmental effects. Existence of critical data gaps will suggest areas in which specific modeling and/or data collection activities should take place. In the second phase, more detailed quantitative risk analyses will be conducted, with residual uncertainties providing the basis for recommending risk mitigation and monitoring activities. We also describe the process used for selecting three cases for fiscal year 2010 risk screening analysis using the ERES. A case is defined as a specific technology deployed in a particular location involving certain environmental receptors specific to that location. The three cases selected satisfy a number of desirable criteria: 1) they correspond to real projects whose deployment is likely to take place in the foreseeable future; 2) the technology developers are willing to share technology and project-related data; 3) the projects represent a diversity of technology-site-receptor characteristics; 4) the projects are of national interest, and 5) environmental effects data may be available for the projects.

  1. Screening method for selecting semiconductor substrates having defects below a predetermined level in an oxide layer

    DOE Patents [OSTI]

    Warren, William L.; Vanheusden, Karel J. R.; Schwank, James R.; Fleetwood, Daniel M.; Shaneyfelt, Marty R.; Winokur, Peter S.; Devine, Roderick A. B.

    1998-01-01

    A method for screening or qualifying semiconductor substrates for integrated circuit fabrication. The method comprises the steps of annealing at least one semiconductor substrate at a first temperature in a defect-activating ambient (e.g. hydrogen, forming gas, or ammonia) for sufficient time for activating any defects within on oxide layer of the substrate; measuring a defect-revealing electrical characteristic of at least a portion of the oxide layer for determining a quantity of activated defects therein; and selecting substrates for which the quantity of activated defects is below a predetermined level. The defect-revealing electrical characteristic may be a capacitance-versus-voltage (C-V) characteristic or a current-versus-voltage (I-V) characteristic that is dependent on an electrical charge in the oxide layer generated by the activated defects. Embodiments of the present invention may be applied for screening any type of semiconductor substrate or wafer having an oxide layer formed thereon or therein. This includes silicon-on-insulator substrates formed by a separation by the implantation of oxygen (SIMOX) process or the bond and etch back silicon-on-insulator (BESOI) process, as well as silicon substrates having a thermal oxide layer or a deposited oxide layer.

  2. Plasma screening effects on the energies of hydrogen atom under the influence of velocity-dependent potential

    SciTech Connect (OSTI)

    Bahar, M. K.

    2014-07-15

    In order to examine the plasma screening and velocity-dependent potential effects on the hydrogen atom, the Schrdinger equation including a more general exponential cosine screened Coulomb and velocity-dependent potential is solved numerically in the framework asymptotic iteration method. The more general exponential cosine screened Coulomb potential is used to model Debye and quantum plasma for the specific values of the parameters in its structure. However, in order to examine effects of velocity-dependent potential on energy values of hydrogen atom in Debye and quantum plasma, the isotropic form factor of velocity-dependent potential is given as harmonic oscillator type, ?(r)=?{sub o}r{sup 2}. Then, the energies of s and p states are calculated numerically without any approximation. In order to investigate thoroughly plasma screening effects and contribution of velocity-dependent potential on energy values of hydrogen atom, the corresponding calculations are carried out by using different values of parameters of more general exponential cosine screened Coulomb potential and isotropic dependence, results of which are discussed.

  3. Yakima River Basin Fish Passage Phase II Fish Screen Construction, Project Completion Report.

    SciTech Connect (OSTI)

    Hudson, R. Dennis

    2008-01-01

    On December 5, 1980, Congress passed the Pacific Northwest Electric Power Planning and Conservation Act (Public Law 96-501). The Act created the Northwest Power Planning Council (now the Northwest Power and Conservation Council). The Council was charged with the responsibility to prepare a Regional Conservation and Electric Power Plan and to develop a program to protect, mitigate, and enhance fish and wildlife including related spawning grounds and habitat on the Columbia River and its tributaries. The Council adopted its Fish and Wildlife Program on November 15, 1982. Section 800 of the Program addresses measures in the Yakima River Basin. The Yakima measures were intended to help mitigate hydroelectric impacts in the basin and provide off-site mitigation to compensate for fish losses caused by hydroelectric project development and operations throughout the Columbia River Basin. The Bonneville Power Administration (BPA) was designated as a major source of funding for such off-site mitigation measures and was requested to initiate discussions with the appropriate Federal project operators and the Council to determine the most expeditious means for funding and implementing the program. The primary measures proposed for rapid implementation in the Yakima River basin were the installation of fish passage and protective facilities. Sec. 109 of The Hoover Power Plant Act of 1984, authorized the Secretary of the Interior to design, construct, operate, and maintain fish passage facilities within the Yakima River Basin. Under Phase I of the program, improvements to existing fish passage facilities and installation of new fish ladders and fish screens at 16 of the largest existing diversion dams and canals were begun in 1984 and were completed in 1990. The Yakima Phase II fish passage program is an extension of the Phase I program. In 1988, the Yakama Nation (YN) submitted an application to amend Sections 803(b) and 1403(4.5) of the Northwest Power and Conservation Council

  4. Screening report on cell materials for high-power Li-Ion HEV batteries.

    SciTech Connect (OSTI)

    Liu, J.; Kahaian, A.; Belharouak, I.; Kang, S.; Oliver, S.; Henriksen, S.; Amine, K.

    2003-04-24

    The Battery Technology Department at Argonne National Laboratory is a major participant in the U.S. Department of Energy's Advanced Technology Development (ATD) program. This multi-national laboratory program is dedicated to improving lithium-ion batteries for high-power HEV and FCEV applications. As part of the FreedomCAR Partnership, this program is addressing the three key barriers for high-power lithium-ion batteries: calendar life, abuse tolerance, and cost. All three of these barriers can be addressed by the choice of materials used in the cell chemistry. To date, the ATD program has developed two high-power cell chemistries, denoted our Gen 1 and Gen 2 cell chemistries. The selection of materials for use in the Gen 2 cell chemistry was based largely on reducing material cost and extending cell calendar life, relative to our Gen 1 cell chemistry. Table 1 provides a list of the materials used in our Gen 2 cell chemistry and their projected costs, when produced in large-scale quantities. In evaluating advanced materials, we have focused our efforts on materials that are lower cost than those listed in Table 1, while simultaneously offering enhanced chemical, structural, and thermal stability. Therefore, we have focused on natural graphite anode materials (having round-edge particle morphologies), cathode materials that contain more Mn and less Co and Ni (which can be produced via low-cost processes), lower cost electrode binders and/or binders that possess superior bonding properties at lower concentrations, and lower cost salts and solvents (with superior thermal and oxidation/reduction stability) for use in the electrolyte. The purpose of this report is to document the results of screening tests that were performed on a large number of advanced low-cost materials. These materials were screened for their potential to impact positively on the calendar life, safety, and/or cost of high-power lithium-ion cell chemistries, relative to our Gen 2 cell chemistry. As

  5. Comparison of three field screening techniques for delineating petroleum hydrocarbon plumes in groundwater at a site in the southern Carson Desert, Nevada

    SciTech Connect (OSTI)

    Smuin, D.R.

    1993-01-01

    Three types of field screening techniques used in the characterization of potentially contaminated sites at Naval Air Station Fallon, Nevada, are compared. The methods and results for each technique are presented. The three techniques include soil-gas surveys, electromagnetic geophysical surveys, and groundwater test hole screening. Initial screening at the first study site included two soil-gas surveys and electromagnetic geophysical studies. These screening methods identified I areas of contamination; however, results were inconclusive. Therefore groundwater test hole screening was performed. Groundwater screening consisted of auger drilling down to the shallow alluvial aquifer. Groundwater samples were collected from the open drill hole with a bailer. On-site head-space analyses for volatile organic compounds (VOCS) were performed using a portable gas chromatograph (GC). Five areas of floating petroleum hydrocarbon product were identified along with the overall dissolved contaminant plume boundaries. Well placement was re-evaluated, and well sites were relocated based on the screening information. The most effective technique for identification of petroleum hydrocarbon-contaminant plumes was groundwater test hole screening. Groundwater screening was subsequently performed at 19 other sites. A total of 450 test holes were analyzed resulting in the delineation of six plumes.

  6. High throughput screening of ligand binding to macromolecules using high resolution powder diffraction

    DOE Patents [OSTI]

    Von Dreele, Robert B.; D'Amico, Kevin

    2006-10-31

    A process is provided for the high throughput screening of binding of ligands to macromolecules using high resolution powder diffraction data including producing a first sample slurry of a selected polycrystalline macromolecule material and a solvent, producing a second sample slurry of a selected polycrystalline macromolecule material, one or more ligands and the solvent, obtaining a high resolution powder diffraction pattern on each of said first sample slurry and the second sample slurry, and, comparing the high resolution powder diffraction pattern of the first sample slurry and the high resolution powder diffraction pattern of the second sample slurry whereby a difference in the high resolution powder diffraction patterns of the first sample slurry and the second sample slurry provides a positive indication for the formation of a complex between the selected polycrystalline macromolecule material and at least one of the one or more ligands.

  7. Anomaly metrics to differentiate threat sources from benign sources in primary vehicle screening.

    SciTech Connect (OSTI)

    Cohen, Israel Dov; Mengesha, Wondwosen

    2011-09-01

    Discrimination of benign sources from threat sources at Port of Entries (POE) is of a great importance in efficient screening of cargo and vehicles using Radiation Portal Monitors (RPM). Currently RPM's ability to distinguish these radiological sources is seriously hampered by the energy resolution of the deployed RPMs. As naturally occurring radioactive materials (NORM) are ubiquitous in commerce, false alarms are problematic as they require additional resources in secondary inspection in addition to impacts on commerce. To increase the sensitivity of such detection systems without increasing false alarm rates, alarm metrics need to incorporate the ability to distinguish benign and threat sources. Principal component analysis (PCA) and clustering technique were implemented in the present study. Such techniques were investigated for their potential to lower false alarm rates and/or increase sensitivity to weaker threat sources without loss of specificity. Results of the investigation demonstrated improved sensitivity and specificity in discriminating benign sources from threat sources.

  8. Method for formation of high quality back contact with screen-printed local back surface field

    DOE Patents [OSTI]

    Rohatgi, Ajeet; Meemongkolkiat, Vichai

    2010-11-30

    A thin silicon solar cell having a back dielectric passivation and rear contact with local back surface field is described. Specifically, the solar cell may be fabricated from a crystalline silicon wafer having a thickness from 50 to 500 micrometers. A barrier layer and a dielectric layer are applied at least to the back surface of the silicon wafer to protect the silicon wafer from deformation when the rear contact is formed. At least one opening is made to the dielectric layer. An aluminum contact that provides a back surface field is formed in the opening and on the dielectric layer. The aluminum contact may be applied by screen printing an aluminum paste having from one to 12 atomic percent silicon and then applying a heat treatment at 750 degrees Celsius.

  9. Use of the dual-screen thru-tubing sand control method

    SciTech Connect (OSTI)

    Freiman, O.H.; Johnson, K.J.

    1995-12-31

    This paper will describe a new sand control method used in area of Ecuador, South America where workover rigs are not readily available. Sand production had been significantly affecting maintenance costs on surface and downhole production equipment and had impacted performance of the downhole jet pump, subsequently causing a decrease in well production. By installing a small-diameter, sintered metal gravel-pack screen across the perforation interval and placing the gravel-pack sand with coiled tubing, the sand production was successfully controlled. The procedure and equipment used to perform this technique as well as post job data that provide results obtained will also be presented. Noteworthy advantages, such as recompletion without removal of the existing production completion string and without use of a workover rig, will also be discussed.

  10. Use of the dual-screen thru-tubing sand control method

    SciTech Connect (OSTI)

    Freiman, O.H.; Johnson, K.J.

    1994-12-31

    This paper describes a new sand control method used in an area of Ecuador, South America where workover rigs are not readily available. Sand production had been significantly affecting maintenance costs on surface and downhole production equipment and had impacted performance of the downhole jet pump, subsequently causing a decrease in well production. By installing a small-diameter, sintered metal gravel-pack screen across the perforation interval and placing the gravel-pack sand with coiled tubing, the sand production was successfully controlled. The procedure and equipment used to perform this technique as well as post job data that provide results obtained is also presented. Noteworthy advantages, such as recompletion without removal of the existing production completion string and without use of a workover rig, is also discussed.

  11. Classical strongly coupled quark-gluon plasma. II. Screening and equation of state

    SciTech Connect (OSTI)

    Gelman, Boris A.; Shuryak, Edward V.; Zahed, Ismail

    2006-10-15

    We analyze the screening and bulk energy of a classical and strongly interacting plasma of color charges, a model we recently introduced for the description of a quark-gluon plasma at T=(1-3)T{sub c}. The partition function is organized around the Debye-Hueckel limit. The linear Debye-Hueckel limit is corrected by a virial expansion. For the pressure, the expansion is badly convergent even in the dilute limit. The nonlinear Debye-Hueckel theory is studied numerically as an alternative for moderately strong plasmas. We use the Debye theory of solid to extend the analysis to the crystal phase at very strong coupling. The analytical results for the bulk energy per particle compare well with the numerical results from molecular dynamics simulations for all couplings.

  12. Synthetic crude oils carcinogenicity screening tests. Progress report, September 15, 1979-March 15, 1980

    SciTech Connect (OSTI)

    Calkins, W.H.; Deye, J.F.; King, C.F.; Hartgrove, R.W.; Krahn, D.F.

    1980-01-01

    Four crude oils (H Coal-Fuel Oil Mode, Occidental in situ Shale Oil, Exxon Donor Solvent Liquid, and SRC II) which were distilled into four fractions (naphtha, mid-distillate, gas oil and residue) for analysis and biological screening testing during the last report period were tested for mutagenicity by the Ames test and for tumor initiating activity by an initiation/promotion (skin painting) test. Substantial agreement exists between Ames and skin painting results. Low boiling naphtha fractions of the 4 crude oils showed little or no mutagenicity or tumor initiating activity by the two tests used. The higher boiling fractions (gas oils and residues) and the crude oils themselves were mutagenic and exhibited tumor initiation activity. The coal derived fractions were more active by both tests than the shale oil fractions.

  13. Visualizations, Screen Shots, and Data Input Files from VisIT

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    VisIt is a free interactive parallel visualization and graphical analysis tool for viewing scientific data on Unix and PC platforms. Users can quickly generate visualizations from their data, animate them through time, manipulate them, and save the resulting images for presentations. VisIt contains a rich set of visualization features so that you can view your data in a variety of ways. It can be used to visualize scalar and vector fields defined on two- and three-dimensional (2D and 3D) structured and unstructured meshes. VisIt was designed to handle very large data set sizes in the terascale range and yet can also handle small data sets in the kilobyte range. The VisIT website provides a gallery of vizualizations, another set of screen shots, and allows downloads of data files for input and source codes and executables for the VisIT software suite.

  14. Use of Time-Aggregated Data in Economic Screening Analyses of Combined Heat and Power Systems

    SciTech Connect (OSTI)

    Hudson II, Carl Randy

    2004-09-01

    Combined heat and power (CHP) projects (also known as cogeneration projects) usually undergo a series of assessments and viability checks before any commitment is made. A screening analysis, with electrical and thermal loads characterized on an annual basis, may be performed initially to quickly determine the economic viability of the proposed project. Screening analyses using time-aggregated data do not reflect several critical cost influences, however. Seasonal and diurnal variations in electrical and thermal loads, as well as time-of-use utility pricing structures, can have a dramatic impact on the economics. A more accurate economic assessment requires additional detailed data on electrical and thermal demand (e.g., hourly load data), which may not be readily available for the specific facility under study. Recent developments in CHP evaluation tools, however, can generate the needed hourly data through the use of historical data libraries and building simulation. This article utilizes model-generated hourly load data for four potential CHP applications and compares the calculated cost savings of a CHP system when evaluated on a time-aggregated (i.e., annual) basis to the savings when evaluated on an hour-by-hour basis. It is observed that the simple, aggregated analysis forecasts much greater savings (i.e., greater economic viability) than the more detailed hourly analysis. The findings confirm that the simpler tool produces results with a much more optimistic outlook, which, if taken by itself, might lead to erroneous project decisions. The more rigorous approach, being more reflective of actual requirements and conditions, presents a more accurate economic comparison of the alternatives, which, in turn, leads to better decision risk management.

  15. Secondary Waste Form Screening Test ResultsCast Stone and Alkali Alumino-Silicate Geopolymer

    SciTech Connect (OSTI)

    Pierce, Eric M.; Cantrell, Kirk J.; Westsik, Joseph H.; Parker, Kent E.; Um, Wooyong; Valenta, Michelle M.; Serne, R. Jeffrey

    2010-06-28

    PNNL is conducting screening tests on the candidate waste forms to provide a basis for comparison and to resolve the formulation and data needs identified in the literature review. This report documents the screening test results on the Cast Stone cementitious waste form and the Geopolymer waste form. Test results suggest that both the Cast Stone and Geopolymer appear to be viable waste forms for the solidification of the secondary liquid wastes to be treated in the ETF. The diffusivity for technetium from the Cast Stone monoliths was in the range of 1.2 10-11 to 2.3 10-13 cm2/s during the 63 days of testing. The diffusivity for technetium from the Geopolymer was in the range of 1.7 10-10 to 3.8 10-12 cm2/s through the 63 days of the test. These values compare with a target of 1 10-9 cm2/s or less. The Geopolymer continues to show some fabrication issues with the diffusivities ranging from 1.7 10-10 to 3.8 10-12 cm2/s for the better-performing batch to from 1.2 10-9 to 1.8 10-11 cm2/s for the poorer-performing batch. In the future more comprehensive and longer term performance testing will be conducted, to further evaluate whether or not these waste forms will meet the regulation and performance criteria needed to cost-effectively dispose of secondary wastes.

  16. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2015-01-01

    An acoustic high-throughput screening method is described for harvesting protein crystals and combining the protein crystals with chemicals such as a fragment library. Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s{sup −1}) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  17. An evaluation of chemical screening test kits for lead in paint

    SciTech Connect (OSTI)

    Oglesby, L.S.

    1996-04-01

    The Residential Lead-Based Paint Hazard Reduction Act (Title X) requires abatement and management of lead-based paint. The purpose of this study was to evaluate three chemical screening test kits using materials and methods from one study and subjecting the results to the statistical analysis of another. The three kits were used to predict the presence of lead in paint at ten weight concentrations from 0.04 to 3.97%. Paint was applied to four wood boards yielding a sample size of 40. Four boards were painted with lead-free paint and used as blanks. All of the boards were tested with the three test kits by an untrained individual having no knowledge of the actual lead content. Sensitivity, specificity, and false positive and negative rates were calculated for the test kit results. The manufactures` detection limits, the observed sensitivity ranged from 1.00 to 0.80, specificity ranged from 1.00 to 0.42, false positive ranged from 0 to 58%, and false negatives ranged from 0 to 20%. At the 0.5% Federal threshold level, the observed sensitivity ranged from 1.00 to 0.94, specificity ranged from 1.00 to 0.5, false positives ranged from 0 to 11.1%, and false negatives ranged from 0 to 20%. The observed false positive and false negative rates for all three kits were found to be significantly lower than those reported in a previous study. These results indicate that the kits perform very well at the Federal threshold, with two of the kits having false negative rates below 12.5% and false positive rates of 3.13%. These results indicate that these two kits would probably be acceptable screening tests for lead in paint.

  18. Fluorescence energy transfer efficiency in labeled yeast cytochrome c: a rapid screen for ion biocompatibility in aqueous ionic liquids

    SciTech Connect (OSTI)

    Baker, Sheila N; Zhao, Hua; Pandey, Siddharth; Heller, William T; Bright, Frank; Baker, Gary A

    2011-01-01

    A fluorescence energy transfer de-quenching assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.

  19. Results of screening activities in salt states prior to the enactment of the Nationall Waste Policy Act

    SciTech Connect (OSTI)

    Carbiener, W.A.

    1983-01-01

    The identification of potential sites for a nuclear waste repository through screening procedures in the salt states is a well-established, deliberate process. This screening process has made it possible to carry out detailed studies of many of the most promising potential sites, and general studies of all the sites, in anticipation of the siting guidelines specified in the Nuclear Waste Policy Act. The screening work completed prior to the passage of the Act allowed the Secretary of Energy to identify seven salt sites as potentially acceptable under the provisions of Section 116(a) of the Act. These sites were formally identified by letters from Secretary Hodel to the states of Texas, Utah, Mississippi, and Louisiana on February 2, 1983. The potentially acceptable salt sites were in Deaf Smith and Swisher Counties in Texas; Davis and Lavender Canyons in the Gibson Dome location in Utah; Richton and Cypress Creek Domes in Mississippi; and Vacherie Dome in Louisiana. Further screening will include comparison of each potentially acceptable site against disqualification factors and selection of a preferred site in each of the three geohydrologic settings from those remaining, in accordance with the siting guidelines. These steps will be documented in statutory Environmental Assessments prepared for each site to be nominated for detailed characterization. 9 references.

  20. Effects of Protective Plates and Stoplogs on Water Flow Through the Gleed Fish Screen Facility, April 2007 - September 2007.

    SciTech Connect (OSTI)

    Chamness, Mickie

    2007-12-03

    In 2007, Pacific Northwest National Laboratory was asked by the U.S. Bureau of Reclamation to provide additional velocity measurements at Gleed fish screens site to support decisions on mitigating extreme flow fluctuations near the screens. The site consistently has had extreme water velocities in places and a strong back eddy at the downstream end in spring and summer. With the help of Washington Department of Fish and Wildlife staff, we measured the effects of different stoplog configurations behind the screens in May and July 2007. Protective metal plates in front of the trash racks were confirmed to be the cause of uneven and extreme water flow past the vertical traveling screens. Stoplogs were not sufficient to significantly reduce the effect of those metal plates on water velocities past and through the site. We provide a few suggestions including making it easier to raise and lower the metal plates and then adjusting them more often, constructing a new trash rack across the diversion entrance, and raising the control gate at the end of the site as long as possible in spring and during flood events.

  1. High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density

    SciTech Connect (OSTI)

    Teplitsky, Ella; Joshi, Karan; Ericson, Daniel L.; Scalia, Alexander; Mullen, Jeffrey D.; Sweet, Robert M.; Soares, Alexei S.

    2015-07-01

    We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. Moreover, a fragment mini-library was screened to observe two known lysozyme We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. A fragment mini-library was screened to observe two known lysozyme

  2. High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Teplitsky, Ella; Joshi, Karan; Ericson, Daniel L.; Scalia, Alexander; Mullen, Jeffrey D.; Sweet, Robert M.; Soares, Alexei S.

    2015-07-01

    We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using thismore » system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. Moreover, a fragment mini-library was screened to observe two known lysozyme We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. A fragment mini-library was screened to observe two known

  3. Screening of CHP Potential at Federal Sites in Select Regions of the U.S.

    SciTech Connect (OSTI)

    Energy Nexus Group, . .

    2002-02-25

    sizing CHP to thermal and electrical estimates. The table below is a summary of findings of CHP potential for those federal facilities that chose to participate in the screening process. The study focused on three U.S. regions: California, Texas, and New York/New England. All federal facilities in these three regions with reported building space greater than 100,000 square feet were initial targets to contact and offer CHP screening services. Ranking criteria were developed to screen sites for near term CHP potential. The potential site list was pared down for a variety of reasons including site- specific and agency wide decisions not to participate, desk audit assessments, and untraceable contact information. The results are based upon the voluntary participation of those sites we were able to contact, so they reflect a fraction of the total potential CHP opportunities at federal government facilities.

  4. A Screening Model to Predict Microalgae Biomass Growth in Photobioreactors and Raceway Ponds

    SciTech Connect (OSTI)

    Huesemann, Michael H.; Van Wagenen, Jonathan M.; Miller, Tyler W.; Chavis, Aaron R.; Hobbs, Watts B.; Crowe, Braden J.

    2013-06-01

    A microalgae biomass growth model was developed for screening novel strains for their potential to exhibit high biomass productivities under nutrient-replete conditions in photobioreactors or outdoor ponds. Growth is modeled by first estimating the light attenuation by biomass according to Beer-Lamberts law, and then calculating the specific growth rate in discretized culture volume slices that receive declining light intensities due to attenuation. The model requires only two physical and two species-specific biological input parameters, all of which are relatively easy to determine: incident light intensity, culture depth, as well as the biomass light absorption coefficient and the specific growth rate as a function of light intensity. Roux bottle culture experiments were performed with Nannochloropsis salina at constant temperature (23 C) at six different incident light intensities (5, 10, 25, 50, 100, 250, and 850 ?mol/m2? sec) to determine both the specific growth rate under non-shading conditions and the biomass light absorption coefficient as a function of light intensity. The model was successful in predicting the biomass growth rate in these Roux bottle cultures during the light-limited linear phase at different incident light intensities. Model predictions were moderately sensitive to minor variations in the values of input parameters. The model was also successful in predicting the growth performance of Chlorella sp. cultured in LED-lighted 800 L raceway ponds operated at constant temperature (30 C) and constant light intensity (1650 ?mol/m2? sec). Measurements of oxygen concentrations as a function of time demonstrated that following exposure to darkness, it takes at least 5 seconds for cells to initiate dark respiration. As a result, biomass loss due to dark respiration in the aphotic zone of a culture is unlikely to occur in highly mixed small-scale photobioreactors where cells move rapidly in and out of the light. By contrast, as supported also by

  5. Preparation and screening of crystalline zeolite and hydrothermally-synthesized materials

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiaodong; Goldwasser, Isy; Briceno, Gabriel; Sun, Xiao-Dong; Wang, Kai-An

    2005-03-08

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  6. Source rock screening studies of Ordovician Maquoketa shale in western Illinois

    SciTech Connect (OSTI)

    Autrey, A.; Crockett, J.E.; Dickerson, D.R.; Oltz, D.F.; Seyler, B.J.; Warren, R.

    1987-09-01

    Rock-Eval (pyrolysis) studies of Ordovician Maquoketa Shale samples (cuttings and cores) from the shallow subsurface (500-800 ft deep) in western Illinois indicate that facies within the Maquoketa have potential as hydrocarbon source rocks. Dark, presumably organic-rich zones within the Maquoketa Shale were selected and analyzed for total organic carbon (TOC), Rock-Eval (pyrolysis), and bulk and clay mineralogy using x-ray diffraction. Preliminary results from six samples from Schuyler, McDonough, and Fulton Counties show TOC values ranging from 4.70% to as high as 12.90%. Rock-Eval parameters, measured by heating organic matter in an inert atmosphere, indicate source rock maturity and petroleum-generative potential. Screening studies, using the Rock-Eval process, describe very good source rock potential in facies of the Maquoketa Shale. Further studies at the Illinois State Geological Survey will expand on these preliminary results. This study complements a proposed exploration model in western Illinois and further suggests the possibility of source rocks on the flanks of the Illinois basin. Long-distance migration from more deeply buried effective source rocks in southern Illinois has been the traditional mechanism proposed for petroleum in basin-flank reservoirs. Localized source rocks can be an alternative to long-distance migration, and can expand the possibilities of basin-flank reservoirs, encouraging further exploration in these areas.

  7. Screening and sheath formation in a nonequilibrium mixed Cairns-Tsallis electron distribution

    SciTech Connect (OSTI)

    Bouzit, Omar; Gougam, Leila Ait; Tribeche, Mouloud

    2015-05-15

    The effects of electron nonextensivity for a given nonthermality state, on Debye shielding and electrostatic sheath formation are examined. A physically meaningful Cairns-Tsallis distribution is outlined and a generalized expression for the Debye screening length λ{sub D}{sup q,α} is obtained. It is shown that an increase of the entropic index q causes λ{sub D}{sup q,α} to decrease whatever the amount of plasma nonthermality α. In addition, smaller pertinent values of q along with relatively higher values of α provide larger values of λ{sub D}{sup q,α}. The shielded electrostatic potential falls off as a function of distance more slowly as α increases, a result somewhat analogous to the dynamical shielding decrease (albeit in a different context) of a free charge as it begins to move. Moreover, smaller pertinent values of q along with relatively higher values of α are found to involve higher ion drift speed v{sub i0} for proper sheath formation. As α increases, the sheath electrostatic potential-gradient dΨ{sub s}/dξ becomes abruptly steep slowing down the energetic electrons leakage to the wall. Moreover, the sheath thickness broadens as the electron nonthermality strengthens.

  8. Methods for transforming and expression screening of filamentous fungal cells with a DNA library

    DOE Patents [OSTI]

    Teter, Sarah; Lamsa, Michael; Cherry, Joel; Ward, Connie

    2015-06-02

    The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.

  9. Linkage approach and direct COL4A5 gene mutation screening in Alport syndrome

    SciTech Connect (OSTI)

    Turco, A.E.; Rossetti, S.; Biasi, O.

    1994-09-01

    Alport Syndrome (AS) is transmitted as an X-linked dominant trait in the majority of families, the defective gene being COL4A5 at Xq22. In the remaining cases AS appears to be autosomally inherited. Recently, mutations in COL4A3 and COL4A4 genes at 2q35-q37 were identified in families with autosomal recessive AS. Mutation detection screening is being performed by non-radioactive single stand conformation polymorphism (SSCP), heteroduplex analysis, and automated DNA sequencing in over 170 AS patients enrolled in the ongoing Italian Multicenter Study on AS. So far twenty-five different mutations have been found, including missense, splicing, and frameshifts. Moreover, by using six tightly linked COL4A5 informative makers, we have also typed two larger AS families, and have shown compatible sex-linked transmission in one other, suggesting autosomal recessive inheritance. In this latter three-generation COL4A5-unlinked family we are now looking for linkage and for mutations in the candidate COL4A3 and COL4A4 genes on chromosome 2q.

  10. Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening

    SciTech Connect (OSTI)

    Xu, Hongbo; Chen, Xiaohong; Huang, Junwei [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Deng, Weiwei [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China)] [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Zhong, Qi [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Yue, Changli [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China)] [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China); Wang, Pingzhang, E-mail: wangpzh@bjmu.edu.cn [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China) [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China); Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Huang, Zhigang, E-mail: enthuangzhigang@sohu.com [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)

    2013-06-21

    Highlights: A novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors was defined. GPR65 was identified to induce the MMP3 expression. GPR65 mediated MMP induction under acidic conditions. AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. GPR65 overexpression can accelerate the invision of A549 cells. -- Abstract: Matrix metalloproteinases (MMPs) are over-expressed in nearly all cancers. To study novel regulatory factors of MMP expression in head and neck cancer (HNC), we screened a total of 636 candidate genes encoding putative human transmembrane proteins using MMP promoter reporter in a dual luciferase assay system. Three genes GPR65, AXL and TNFRSF10B dramatically activated the induction of MMP3 expression. The induction of MMP expression by GPR65 was further confirmed in A549 and/or FaDu cells. GPR65 mediated MMP induction under acidic conditions. The AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. Moreover, the A549 cells infected by recombinant adenovirus of GPR65 showed accelerated cell invasion. In conclusion, we validate that GPR65 is vital regulatory genes upstream of MMP3, and define a novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors.

  11. White Oak Creek Embayment site characterization and contaminant screening analysis. Environmental Restoration Program

    SciTech Connect (OSTI)

    Blaylock, B.G.; Ford, C.J.; Frank, M.L.; Hoffman, F.O.; Hook, L.A.

    1993-01-01

    Analyses of sediment samples collected near the mouth of White Oak Creek during the summer of 1990 revealed {sup 137}Cs concentrations [> 10{sup 6} Bq/kg dry wt (> 10{sup 4} pCi/g dry wt)] near the sediment surface. Available evidence indicates that these relatively high concentrations of {sup 137}Cs now at the sediment surface were released from White Oak Dam in the mid-1950s and had accumulated at depositionalsites in the embayment. These accumulated sediments are being eroded and transported downstream primarily during winter low-water levels by flood events and by a combination of normal downstream flow and the water turbulence created by the release of water from Melton Hill Dam during hydropower generation cycles. This report provides a more thorough characterization of the extent of contamination in WOCE than was previously available. Environmental samples collected from WOCE were analyzed for organic, inorganic, and radiological contaminants in fish, water, and sediment. These results were used to conduct a human health effects screening analysis. Walkover radiation surveys conducted inside the fenced area surrounding the WOCE at summer-pool (741 ft MSL) and at winter-pool (733 ft MSL) level, indicated a maximum exposure rate of 3 mR h{sup 1} 1 m above the soil surface.

  12. Screening Analysis for EPACT-Covered Commercial HVAC and Water-Heating Equipment

    SciTech Connect (OSTI)

    Somasundaram, Sriram; Armstrong, Peter R.; Belzer, David B.; Gaines, Suzanne C.; Hadley, Donald L.; Katipumula, S.; Smith, David L.; Winiarski, David W.

    2000-04-25

    The Energy Policy and Conservation Act (EPCA) as amended by the Energy Policy Act of 1992 (EPACT) establishes that the U.S. Department of Energy (DOE) regulate efficiency levels of certain categories of commercial heating, cooling, and water-heating equip-ment. EPACT establishes the initial minimum efficiency levels for products falling under these categories, based on ASHRAE/IES Standard 90.1-1989 requirements. EPCA states that, if ASHRAE amends Standard 90.1-1989 efficiency levels, then DOE must establish an amended uniform national manufacturing standard at the minimum level specified in the amended Standard 90.1 and that it can establish higher efficiency levels if they would result in significant additional energy savings. Standard 90.1-1999 increases minimum efficiency levels for some of the equipment categories covered by EPCA 92. DOE conducted a screening analysis to determine the energy-savings potential for EPACT-covered products meet and exceeding these levels. This paper describes the methodology, data assumptions, and results of the analysis.

  13. Location performance objectives for the NNWSI area-to-location screening activity

    SciTech Connect (OSTI)

    Sinnock, S.; Fernandez, J.A.

    1984-01-01

    Fifty-four objectives were identified to guide the screening of the Nevada Research and Development Area of the Nevada Test Site for relatively favorable locations for the disposal of nuclear waste in a mined geologic repository. The objectives were organized as a hierarchy composed of 4 upper-level, 12 middle-level, and 38 lower-level objectives. The four upper-level objectives account for broad national goals to contain and isolate nuclear waste in an environmentally sound and economically acceptable manner. The middle-level objectives correspond to topical categories that logically relate the upper-level objectives to site-specific concerns such as seismicity, sensitive species, and flooding hazards (represented by the lower-level objectives). The relative merits of alternative locations were compared by an application of decision analysis based on standard utility theory. The relative favorabilities of pertinent physical conditions at each alternative location were weighted in relation to the importance of objectives, and summed to produce maps indicating the most and the least favorable locations. Descriptions of the objectives were organized by the hierarchical format; they detail the applicability of each objective to geologic repository siting, previously published siting criteria corresponding to each objective, and the rationale for the weight assigned to each objective, and the pertinent attributes for evaluating locations with respect to each objective. 51 references, 47 figures, 4 tables.

  14. Cell wall composition and biomass recalcitrance differences within a genotypically diverse set of Brachypodium distachyon inbred lines

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cass, Cynthia L.; Lavell, Anastasiya A.; Santoro, Nicholas; Foster, Cliff E.; Karlen, Steven D.; Smith, Rebecca A.; Ralph, John; Garvin, David F.; Sedbrook, John C.

    2016-05-26

    Brachypodium distachyon (Brachypodium) has emerged as a useful model system for studying traits unique to graminaceous species including bioenergy crop grasses owing to its amenability to laboratory experimentation and the availability of extensive genetic and germplasm resources. Considerable natural variation has been uncovered for a variety of traits including flowering time, vernalization responsiveness, and above-ground growth characteristics. However, cell wall composition differences remain underexplored. Therefore, we assessed cell wall-related traits relevant to biomass conversion to biofuels in seven Brachypodium inbred lines that were chosen based on their high level of genotypic diversity as well as available genome sequences and recombinantmore » inbred line (RIL) populations. Senesced stems plus leaf sheaths from these lines exhibited significant differences in acetyl bromide soluble lignin (ABSL), cell wall polysaccharide-derived sugars, hydroxycinnamates content, and syringyl:guaiacyl:p-hydroxyphenyl (S:G:H) lignin ratios. Free glucose, sucrose, and starch content also differed significantly in senesced stems, as did the amounts of sugars released from cell wall polysaccharides (digestibility) upon exposure to a panel of thermochemical pretreatments followed by hydrolytic enzymatic digestion. Correlations were identified between inbred line lignin compositions and plant growth characteristics such as biomass accumulation and heading date (HD), and between amounts of cell wall polysaccharides and biomass digestibility. Finally, stem cell wall p-coumarate and ferulate contents and free-sugars content changed significantly with increased duration of vernalization for some inbred lines. Taken together, these results show that Brachypodium displays substantial phenotypic variation with respect to cell wall composition and biomass digestibility, with some compositional differences correlating with growth characteristics. Moreover, besides influencing HD and biomass accumulation, vernalization was found to affect cell wall composition and free sugars accumulation in some Brachypodium inbred lines, suggesting genetic differences in how vernalization affects carbon flux to polysaccharides. Lastly, the availability of related RIL populations will allow for the genetic and molecular dissection of this natural variation, the knowledge of which may inform ways to genetically improve bioenergy crop grasses.« less

  15. 3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions

    SciTech Connect (OSTI)

    Wenzel, Carsten; Riefke, Björn; Gründemann, Stephan; Krebs, Alice; Christian, Sven; Prinz, Florian; Osterland, Marc; Golfier, Sven; Räse, Sebastian; Ansari, Nariman; Esner, Milan; Bickle, Marc; Pampaloni, Francesco; Mattheyer, Christian; Stelzer, Ernst H.; Parczyk, Karsten; Prechtl, Stefan; Steigemann, Patrick

    2014-04-15

    Cancer cells in poorly vascularized tumor regions need to adapt to an unfavorable metabolic microenvironment. As distance from supplying blood vessels increases, oxygen and nutrient concentrations decrease and cancer cells react by stopping cell cycle progression and becoming dormant. As cytostatic drugs mainly target proliferating cells, cancer cell dormancy is considered as a major resistance mechanism to this class of anti-cancer drugs. Therefore, substances that target cancer cells in poorly vascularized tumor regions have the potential to enhance cytostatic-based chemotherapy of solid tumors. With three-dimensional growth conditions, multicellular tumor spheroids (MCTS) reproduce several parameters of the tumor microenvironment, including oxygen and nutrient gradients as well as the development of dormant tumor regions. We here report the setup of a 3D cell culture compatible high-content screening system and the identification of nine substances from two commercially available drug libraries that specifically target cells in inner MCTS core regions, while cells in outer MCTS regions or in 2D cell culture remain unaffected. We elucidated the mode of action of the identified compounds as inhibitors of the respiratory chain and show that induction of cell death in inner MCTS core regions critically depends on extracellular glucose concentrations. Finally, combinational treatment with cytostatics showed increased induction of cell death in MCTS. The data presented here shows for the first time a high-content based screening setup on 3D tumor spheroids for the identification of substances that specifically induce cell death in inner tumor spheroid core regions. This validates the approach to use 3D cell culture screening systems to identify substances that would not be detectable by 2D based screening in otherwise similar culture conditions. - Highlights: • Establishment of a novel method for 3D cell culture based high-content screening. • First reported high

  16. Movement of Fall Chinook Salmon Fry Oncorhynchus Tshawytscha : A Comparison of Approach Angles for Fish Bypass in a Modular Rotary Drum Fish Screen.

    SciTech Connect (OSTI)

    Neitzel, D.A.; Blanton, S.L.; Abernethy, C. Scott; Daly, D.S.

    1996-08-01

    The Pacific Northwest National Laboratory (PNNL) performed tests to determine whether a significant difference in fish passage existed between a 6-ft screening facility built perpendicularly to canal flow and an identical screening facility with the screen mounted at a 45-degree angle to the approach channel. A modular drum screen built by the Washington Department of Fish and Wildlife was installed at PNNL`s Aquatic Ecology research laboratory in Richland, Washington. Fall chinook salmon fry were introduced into the test system, and their movements were monitored. A total of 14 tests (400 fish per test) that lasted 20 hours were completed during April and May, 1996. There was no significant difference in fish passage rate through the two approach configurations. Attraction flow to the bypass across the face of the screen was more evident for the angled approach, although this did not appear to play a significant role in attracting fish to the bypass. Approach velocities at the face of the screen did not exceed the 0.4 fps criteria for either approach configuration and posed not threat to fish. No fish passed over, around, or through the drum screen during any test.

  17. Screening Assessment of Potential Human-Health Risk from Future Natural-Gas Drilling Near Project Rulison in Western Colorado

    SciTech Connect (OSTI)

    Daniels Jeffrey I.,Chapman Jenny B.

    2012-01-01

    The Project Rulison underground nuclear test was conducted in 1969 at a depth of 8,400 ft in the Williams Fork Formation of the Piceance Basin, west-central Colorado (Figure 1). The U.S. Department of Energy Office of Legacy Management (LM) is the steward of the site. Their management is guided by data collected from past site investigations and current monitoring, and by the results of calculations of expected behavior of contaminants remaining in the deep subsurface. The purpose of this screening risk assessment is to evaluate possible health risks from current and future exposure to Rulison contaminants so the information can be factored into LM's stewardship decisions. For example, these risk assessment results can inform decisions regarding institutional controls at the site and appropriate monitoring of nearby natural-gas extraction activities. Specifically, the screening risk analysis can provide guidance for setting appropriate action levels for contaminant monitoring to ensure protection of human health.

  18. Evaluation and Screening of Remedial Technologies for Uranium at the 300-FF-5 Operable Unit, Hanford Site, Washington

    SciTech Connect (OSTI)

    Nimmons, Michael J.

    2007-08-01

    Pacific Northwest National Laboratory (PNNL) is presently conducting a re-evaluation of remedies addressing persistent dissolved uranium concentrations in the upper aquifer under the 300 Area of the Hanford Site in southeastern Washington State. This work is being conducted as a Phase III feasibility study for the 300-FF-5 Operable Unit on behalf of the U.S. Department of Energy. As part of the feasibility study process, a comprehensive inventory of candidate remedial technologies was conducted by PNNL. This report documents the identification and screening of candidate technologies. The screening evaluation was conducted in accordance with guidance and processes specified by U.S. Environmental Protection Agency regulations associated with implementation of the Comprehensive Environmental Response, Compensation, and Liability Act process.

  19. The TSCA interagency testing committee`s approaches to screening and scoring chemicals and chemical groups: 1977-1983

    SciTech Connect (OSTI)

    Walker, J.D.

    1990-12-31

    This paper describes the TSCA interagency testing committee`s (ITC) approaches to screening and scoring chemicals and chemical groups between 1977 and 1983. During this time the ITC conducted five scoring exercises to select chemicals and chemical groups for detailed review and to determine which of these chemicals and chemical groups should be added to the TSCA Section 4(e) Priority Testing List. 29 refs., 1 fig., 2 tabs.

  20. Use of thermal desorption/gas chromatography as a performance-based screening method for petroleum hydrocarbons

    SciTech Connect (OSTI)

    Slavin, P.J. |; Crandall, K.; Dawson, L.; Kottenstette, R.; Wade, M. |

    1996-08-01

    Thermal desorption/gas chromatography (TD/GC) was used to screen soil samples on site for total petroleum hydrocarbon (TPH) content during a RCRA Facility Investigation (RFI). It proved to be a rapid, cost- effective tool for detecting non-aromatic mineral oil in soil. The on- site TD/GC results correlated well with those generated at an off- site laboratory for samples analyzed in accordance with EPA Method 418.1.