National Library of Energy BETA

Sample records for laura roberts biological

  1. Sandia Energy - Laura Cutler

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laura Cutler Home Laura Cutler Laura Cutler Student Intern Department: Systems Modeling lauracutleroriginal-285ca826adc948681032552cbfebc533...

  2. Laura Millsaps

    Broader source: Energy.gov [DOE]

    Laura Millsaps is a Communication Specialist at the Ames Laboratory, one of the Department of Energy's 17 National Laboratories. 

  3. Laura Garchar

    Broader source: Energy.gov [DOE]

    Laura Garchar is a Science and Technology Policy Fellow through DOE's Institute for Science and Education at Oak Ridge, Tennessee (ORISE). She has a B.S. in Geological Engineering from the...

  4. Laura Smith Morton

    Broader source: Energy.gov [DOE]

    Laura Smith Morton is a Senior Advisor to the Director of Energy Policy and Systems Analysis.  Laura previously served as a Policy Advisor in the Office of Management and Budget (OMB) from...

  5. Laura Mascio Kegelmeyer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mascio Kegelmeyer Finding Her Own Beat Laura Mascio Kegelmeyer dancing An avid ballroom dancer, Laura dances with the instructor at a tango class at LLNL. If Laura Mascio Kegelmeyer's parents had simply stayed put, like generations before them, her life would likely have turned out quite differently. Laura's parents, and several of her older siblings, hailed from a fairly remote village in central Italy. During World War II, the village was destroyed and the family lost many family members and

  6. Laura Wilkerson | Department of Energy

    Energy Savers [EERE]

    Laura Wilkerson About Us Laura Wilkerson - Director, Planning and Execution Division Laura Wilkerson Laura Wilkerson is the Director of the Planning and Execution Division for the Department of Energy's (DOE) Oak Ridge Office of Environmental Management (OREM) Program Office in Oak Ridge, Tennessee. She has 24 years of experience directing and managing technical programs and projects for DOE. She is responsible for the planning and execution of cleanup at Oak Ridge's three primary sites-the East

  7. Laura Scheele | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laura Scheele About Us Laura Scheele - Idaho National Laboratory Laura Scheele is the Stakeholder and Media Affairs Lead on the Partnerships, Engagement and Tech Deployment team at Idaho National Laboratory, one of the Department of Energy's 17 National Laboratories. Most Recent Tomorrow's Nuclear Reactors are Closer Than You Think March 1

  8. Laura Gagliardi | Center for Gas SeparationsRelevant to Clean...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laura Gagliardi Previous Next List Laura Gagliardi Laura Gagliardi Professor of Chemistry, University of Minnesota, Minneapolis Email: gagliard at umn.edu Phone: 612-625-8299...

  9. Robert Neylan

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Neylan Robert Neylan Robert-Neyland.jpg Robert J. Neylan HPC Technician Operations Technology Group RJNeylan@lbl.gov Phone: (510) 486-6821 Fax: (510) 486-4316 1 Cyclotron Road Mail Stop 943-256 Berkeley, CA 94720 Last edited: 2014-02-26 16:11:42

  10. Signature of Robert Poole Signature of Robert Poole Signature of Robert Poole

    National Nuclear Security Administration (NNSA)

    Robert Poole Signature of Robert Poole Signature of Robert Poole

  11. Robert Lange

    Broader source: Energy.gov [DOE]

    As Deputy Assistant Secretary for Corporate Business Operations, Robert Lange is responsible for all personnel, budget, and technical oversight activities within the Office of Nuclear Energy. Mr....

  12. Jesse Roberts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jesse Roberts - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs Advanced Nuclear

  13. Laura E. Ratcliff | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    E. Ratcliff Postdoctoral Appointee - Electronic Structure Code Laura Ratcliff Argonne National Laboratory 9700 South Cass Avenue Bldg. 240 - Wkstn. 1D15 Argonne, IL 60439 630-252-1027 lratcliff@anl.gov Areas of Interest: Ab Initio Electronic Structure Methods

  14. Robert Erck | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Photo of Robert Erck Robert Erck Metallurgist E-mail erck

  15. Dr. Robert Blankenship | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Blankenship September 10, 2011 Dr. Robert Blankenship "Solar Energy and Photosynthetic Antenna Research" Published: September 10, 2011 Dr. Robert Blankenship, Lucille P. Markey Distinguished Professor of Arts and Sciences, Departments of Biology and Chemistry, gives his talk entitled "Solar Energy and Photosynthetic Antenna Research". Original Event Info: Tuesday, September 6, 2011 Washington University Seigle L006 4:00 pm - 5:00 pm

  16. Robert Jilek: Pellion Technologies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Jilek: Pellion Technologies Alumni Link: Opportunities, News and Resources for Former Employees Latest Issue:September 2015 all issues All Issues » submit Robert Jilek: Pellion Technologies Senior research scientist at eastern energy storage startup September 3, 2014 Robert Jilek Robert Jilek Contact Linda Anderman Email Robert Jilek Jilek is currently with Pellion Technologies Bob Jilek is currently spending part of his time in a management role at Pellion Technologies in the Cambridge

  17. Robert J. Osborn

    Broader source: Energy.gov [DOE]

    Robert J. Osborn II, a member of the Senior Executive Service, is currently the Associate Administrator for Information Management and Chief Information Officer for the National Nuclear Security...

  18. Dave Roberts POBox

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    .*..* -. - Dave Roberts POBox 524 Richland, WA 99352 September 10,2012 Office of River Protection POBox 550 Richland, WA 99352 Re: Freedom of Information Request Dear Sir or...

  19. Robert H. Jones Jr.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert H. Jones Jr. October 2014 SRNL-TR-2014-00221, Revision 0 Feasibility and ... Approval by the Department of Energy prior to release is required. Reviewed by: R. H. ...

  20. Robert M. Lingan

    Broader source: Energy.gov [DOE]

    Robert Lingan is the Director of the Office of Corporate Security Strategy, Analysis and Special Operations within the Office of the Associate Under Secretary for Environment, Health, Safety and...

  1. Robert M. Bestani

    Broader source: Energy.gov [DOE]

    Mr. Robert (Bob) M. Bestani is Director, Risk Management Division, providing leadership on LPO risk management and enterprise compliance activities. As Chairman of the Credit Committee and the Risk...

  2. Laura Gagliardi | Center for Gas SeparationsRelevant to Clean Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technologies | Blandine Jerome Laura Gagliardi Previous Next List Laura Gagliardi Laura Gagliardi Professor of Chemistry, University of Minnesota, Minneapolis Email: gagliard [at] umn.edu Phone: 612-625-8299 EFRC research: Laura Gagliardi is an Associated Investigator involved in quantum chemical calculations of MOFs and their interactions with gas molecules. EFRC publications: Vlaisavljevich, Bess; Odoh, Samuel O.; Schnell, Sondre K.; Dzubak, Allison; Lee, Kyuho; Planas, Nora; Neaton,

  3. Robert Berger > Asst. Professor - Western Washington University...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Berger Asst. Professor - Western Washington University robert.berger@wwu.edu Formerly a member of the Neaton Group at the Lawrence Berkeley Laboratory, Robert finished his...

  4. Rob Roberts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roberts About Us Rob Roberts - Former Director of Digital Strategy Rob Roberts Rob Roberts is the former Director of Digital Strategy for the U.S. Department of Energy. Prior to joining Energy, Rob worked in a number of digital roles throughout government, academia and journalism, including positions in the White House Office of Digital Strategy, USA Today, and the Raleigh News & Observer. He also teaches digital media and video as an adjunct professor at American University. Rob received a

  5. Robert Corbin | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert Corbin About Us Robert Corbin - Deputy Assistant Secretary, Office of Petroleum Reserves Robert Corbin Mr. Robert Corbin is the Deputy Assistant Secretary for the Office of Petroleum Reserves in the DOE Office of Fossil Energy. Mr. Corbin is responsible for providing executive leadership and program direction in the management and operation of the Department of Energy's Strategic Petroleum Reserve, Northeast Home Heating Oil Reserve, and Naval Petroleum and Oil Shale Reserve. Prior to

  6. Robert Marlay | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert Marlay About Us Robert Marlay - Director, Office of International Science and Technology Collaboration Robert Marlay Dr. Robert Marlay is a member of the U.S. Government's Senior Executive Service. He is currently Director, Office of International Science and Technology Collaboration. He serves concurrently as U.S. Director of the U.S.-China Clean Energy Research Center, a bilateral initiative to encourage R&D collaboration and accelerate advanced technology development and deployment

  7. Carmichael Roberts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Carmichael Roberts About Us Carmichael Roberts - General Partner, North Bridge Venture Partners Carmichael Roberts Carmichael Roberts is a General Partner at North Bridge Venture Partners where he finances and builds companies that make new products using chemistry, materials science and/or materials engineering. He primarily focuses on early stage ventures, including helping founders launch companies from initial formation. Carmichael lead the North Bridge investment in 1366 technologies, a

  8. Robert Gee | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert Gee About Us Robert Gee - President and Founder, Gee Strategies Group Robert Gee Robert W. Gee is President of Gee Strategies Group LLC, a consulting firm providing policy analysis, advocacy, and litigation support services for the energy, electric utility and critical infrastructure industries based in Washington, D.C. A thought leader for the energy industry for over 30 years, his diverse client base includes investors, trade associations, utilities, and public sector institutions. He

  9. ATTENDEEES: Ashley Armstrong, DOE Laura Barhydt DOE Eric Stas, DOE

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ATTENDEEES: Ashley Armstrong, DOE Laura Barhydt DOE Eric Stas, DOE Francine Pinto, DOE R. Bruce Carnevale, Bradford White Corporation Mark Taylor, Bradford White Corporation Adam S. Olsen, Sconset Strategies, LLC on behalf of Bradford White Corporation MEETING DATE: Tuesday, March 24, 2015 SUBJECTS DISCUSSED: Notice of proposed rulemaking establishing a mathematical conversion factor for consumer water heaters and certain commercial water heaters, specifically: 1.) NOPR release date and the

  10. Patents -- Robert Hofstadter

    Office of Scientific and Technical Information (OSTI)

    Patents - Robert Hofstadter Hofstadter Page * Resources with Additional Information US 3,373,279 EUROPIUM ACTIVATED STRONTIUM IODIDE SCINTILLATORS - Hofstadter, R.; March 12, 1968 This invention comprises a scintillation crystal composed of strontium iodide activated by europium. The invention also comprises a radiation detector having a detecting element and a chamber optically coupled to this detecting element, having within the chamber a scintillator consisting of strontium iodide activated

  11. Robert Howard Grubbs - JCAP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    People Profiles Harry A. Atwater Theodor Agapie Joel W. Ager III Alexis T. Bell Marco Bernardi Bruce Brunschwig Walter Drisdell William A. Goddard III John Gregoire Robert Howard Grubbs Joel A. Haber Martin P. Head-Gordon John C. Hemminger Frances A. Houle Thomas Jaramillo Clifford P. Kubiak Hans Joachim Lewerenz Nathan S. Lewis Daniel J. Miller Thomas Francis Miller III Jeffrey B. Neaton Jens K. Norskov Kristin A. Persson Jonas C. Peters Ian D. Sharp Manuel P. Soriaga Francesca Maria Toma F.

  12. Dr. Robert Tribble

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Tribble You may reach me at Office: Cyclotron (between ZAC and BLOC) Phone: 845-1411 Email: tribble@comp.tamu.edu Information about me: I have been teaching at Texas A&M since 1975. I carry out research in weak interactions, nuclear reactions at low and high energy and nuclear astrophysics. I received my Ph.D. degree from Princeton in 1973. My wife, Janice teaches math here. We have two children, Matthew (age 9) and Jennifer (age 7). Class information: Physics 304

  13. Robert Winarski | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Winarski Physicist Robert Winarski was responsible for the design and construction of Argonne National Laboratory's X-ray Nanoprobe Beamline Project. He conceived of and implemented innovative synchrotron radiation experiments and techniques specifically related to the Nanoprobe. Robert is a scientist in the X-ray Microscopy Group at the Center for Nanoscale Materials and is the leader of the nanotomography program at the Hard X-ray Nanoprobe Beamline (Sector 26). The Hard X-ray Nanoprobe

  14. Robert Bectel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert Bectel About Us Robert Bectel - Senior Program Analyst, for Data Management and Digital Communications A senior technology executive, Robert currently serves as Senior Program Analyst, for the Office of Technology Transitions (OTT) where he helps accelerate the commercialization of energy solutions. Prior to joining OTT, Rob served four years as the Chief Technology Officer for the Office of Energy Efficiency at the Department of Energy. He seeks to establish strong enterprise IT

  15. Robert Sandoli | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert Sandoli About Us Robert Sandoli - Director, International Photo of Robert Sandoli Rob Sandoli is the Director for EERE's International portfolio. He leads a team focused on increasing the speed and scale of clean energy deployment through international collaboration with strategic partners. He manages EERE bilateral and multilateral partnerships and programs, develops new international collaborations, and coordinates international efforts with other DOE offices, federal agencies, and

  16. Robert Jacob | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Climate Scientist Robert Jacob is a computational climate scientist in the Mathematics and Computer Science Division of Argonne National Laboratory and a Fellow in the...

  17. Robert Scott | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert Scott Technical Support Analyst Robert Scott Argonne National Laboratory 9700 South Cass Avenue Building 240 - Wkstn. 2D15 Argonne, IL 60439 630-252-5745 scott...

  18. Robert Gordon University | Open Energy Information

    Open Energy Info (EERE)

    Gordon University Jump to: navigation, search Name: Robert Gordon University Address: Centre for Research in Energy and the Environment The Robert Gordon University Schoolhill...

  19. USCA Convocation Center 375 Robert ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1, 2013 USCA Convocation Center 375 Robert M. Bell Parkway, Aiken, SC 29801 Cash Bar Reception 6:00-6:45 pm BanquetLecture 7:00 pm Tickets: 50 per person Must be...

  20. Robert Graham | Department of Energy

    Energy Savers [EERE]

    Robert Graham About Us Robert Graham - Director, EV Everywhere Challenge Director, EV Everywhere Challenge, US Department of Energy, responsible for the DOE's initiatives to increase market penetration of plug-in electric vehicles. Upon retirement from Southern California Edison in January 2014, where he served as a member of the SCE transportation electrification program, Bob has supported the market expansion of plug in electric vehicles as a part time consultant. Engagement in the PEV market

  1. Robert B. Laughlin and the Fractional Quantum Hall Effect

    Office of Scientific and Technical Information (OSTI)

    Robert B. Laughlin and the Fractional Quantum Hall Effect Resources with Additional Information Robert B. Laughlin Photo Courtesy of LLNL Robert B. Laughlin shared the 1998 Nobel...

  2. J. Robert Schrieffer and the BCS Theory of Superconductivity

    Office of Scientific and Technical Information (OSTI)

    J. Robert Schrieffer and the BCS Theory of Superconductivity Resources with Additional Information J. Robert Schrieffer Courtesy of NHMFL Robert Schrieffer received his BS from ...

  3. Nathan V. Roberts | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nathan V. Roberts Postdoctoral Appointee Nathan V. Roberts Argonne National Laboratory 9700 South Cass Avenue Building 240 - Wkstn. 1D18 Argonne, IL 60439 630-252-0036 nvroberts@alcf.anl

  4. Robert C. Marcum | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert C. Marcum About Us Robert C. Marcum - Director of Portfolio Management, LPO Robert Marcum Portrait Mr. Robert Marcum is Director of Portfolio Management for the Department of Energy's Loan Programs Office. Mr. Marcum is a senior manager with over 20 years of experience in project and corporate finance as well as project development as both lender and sponsor. Currently, he leads LPO activities to manage the $30 billion clean energy and advanced vehicle manufacturing portfolio including

  5. Robert E. Edwards, III | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert E. Edwards, III About Us Robert E. Edwards, III - Manager (Acting) & Deputy Manager Robert E. Edwards, III Mr. Robert Edwards currently serves as Acting Manager of the Department of Energy's Portsmouth/Paducah Project Office (PPPO) in Lexington, Kentucky. He joined PPPO as Deputy Manager on December 30, 2012. PPPO is charged with the environmental remediation, deactivation, and decontamination and decommissioning of the former gaseous diffusion plants at Paducah, KY and Portsmouth,

  6. NREL: Energy Analysis - Billy J. Roberts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Billy J. Roberts Photo of Billy Roberts Billy J. Roberts is a member of the Data Analysis and Visualization Group in the Strategic Energy Analysis Center. Scientist III - GIS On staff since 2007 Phone number: 303-275-3824 E-mail: billy.roberts@nrel.gov Areas of expertise Spatial analysis and cartography Primary research interests Renewable energy and cartographic technique Wildlife and energy technology interactions Geothermal technology Education and background training M.S. Certificate in

  7. Glenn Roberts Jr. | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Glenn Roberts Jr. About Us Glenn Roberts Jr. - Berkeley Lab Glenn Roberts Jr. is a science writer at Berkeley Lab, one of the Department of Energy's 17 National Laboratories. Most Recent New Galaxy-Hunting Sky Camera Sees Redder Better March 9

  8. Laura H. Greene, 1998 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laura H. Greene, 1998 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1990's Laura H. Greene, 1998 Print Text Size: A A A FeedbackShare Page For her research in novel

  9. Robert G. Green | Department of Energy

    Energy Savers [EERE]

    Robert G. Green About Us Robert G. Green - Principal Deputy CIO for Enterprise Information Resources Management | Acting Deputy CIO for Architecture Engineering, Technology & Innovation Mr. Robert Green serves as Principal Deputy for Enterprise Information Resources Management, focusing on enterprise information management and technology, to include strategy, policy and standards and cyber coordination across the extended DOE enterprise. Prior to his role as Principal Deputy, Mr. Green

  10. Robert Kaita | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Principal Research Physicist, P.I., LTX Robert (Bob) Kaita is the head of plasma diagnostic operations and acting head of boundary physics operations for the National Spherical...

  11. Robert G Andre | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    G Andre Senior Computational Scientist Dr. Robert Andre is currently a member of the Computational Plasma Physics Group at the Princeton Plasma Physic Laboratory (PPPL) where he...

  12. NREL: Energy Analysis - Robert M. Margolis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert M. Margolis Photo of Robert M. Margolis Robert M. Margolis is the group manager of the Washington D.C. Office in the Strategic Energy Analysis Center. Senior Analyst (Group Manager) On staff since April 2003 Phone number: 202-488-2222 E-mail: robert.margolis@nrel.gov Areas of expertise Energy-economic-environmental modeling, including national and global-scale models Economic and market analysis of renewable energy technologies R&D planning and evaluation Long-term scenario

  13. Dr. Laura Biven | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Dr. Laura Biven Deputy Director for Science Programs Deputy Director Home Mission & Functions Deputy Director Biography Organization Staff Presentations & Testimony Federal Advisory Committees Committees of Visitors Contact Information Deputy Director for Science Programs U.S. Department of Energy SC-2/Forrestal Building 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 F: (202) 586-4120 E: Email Us U.S. Department of Energy SC-2/Germantown Building 1000 Independence

  14. To: Laura Barhydt, U.S. Department of Energy From: Amy Shepherd, General Counsel, AHRI

    Energy Savers [EERE]

    December 21, 2015 To: Laura Barhydt, U.S. Department of Energy From: Amy Shepherd, General Counsel, AHRI Re: Ex Parte Communication on Department of Energy Guidance for Dry Charged R- 22 Air Conditioning Units and Proposed Test Procedure Language on Units With No Match On December 1, 2015, AHRI staff and representatives of stakeholders who manufacture central air conditioning and heat pump systems met with representatives from the Department of Energy (DOE) to seek clarification and guidance on

  15. To: Laura Barhydt, United States Department of Energy John Cymbalsky, United States Department of Energy

    Energy Savers [EERE]

    September 25, 2015 To: Laura Barhydt, United States Department of Energy John Cymbalsky, United States Department of Energy From: Amy Shepherd, General Counsel, AHRI Re: Ex Parte Communication on Central Air-Conditioner Test Procedure On September 18, 2015, AHRI staff and Manufacturing Representatives met with representatives from the Department of Energy (DOE) to discuss proposed amended test procedures for central air conditioners. The meeting was held at AHRI offices in Arlington, Virginia. A

  16. NREL: Biomass Research - Robert M. Baldwin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert M. Baldwin Photo of Robert M. Baldwin. Robert Baldwin is a Principal Scientist in the National Bioenergy Center at the National Renewable Energy Laboratory (NREL) in Golden, Colorado; he has worked at NREL since 2008. Dr. Baldwin holds the degrees Bachelor of Science and Master of Science in chemical engineering from Iowa State University and the PhD degree in chemical engineering from the Colorado School of Mines (CSM). Prior to joining NREL, Bob spent 30 years as a faculty member at CSM

  17. Robert J. Thompson | Department of Energy

    Energy Savers [EERE]

    Robert J. Thompson About Us Robert J. Thompson - EMAB Board Member Robert J. Thompson is Chairman of the Energy Communities Alliance, a national coalition of cities and counties located adjacent to Department of Energy facilities. Mr. Thompson has also served on the Richland City Council since January 1994 and as Mayor from January 2000 to January 2004. He received a B.A. in Political Science from Washington State University and a J.D. from Willamette Law School. He is currently in private

  18. Robert C. Gibbs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Robert C. Gibbs About Us Robert C. Gibbs - Chief Human Capital Officer Robert C. Gibbs Bob Gibbs was born and raised in Boston, Massachusetts. A retired naval officer, he holds both a B.A. in business management from the University of Washington, and a J.D. from George Mason University. He is a member of the Maryland and the American Bar Associations. Mr. Gibbs completed the nuclear training pipeline and served at sea onboard the USS DANIEL WEBSTER (BLUE) (SSBN 626), USS HENRY L. STIMSON (GOLD)

  19. Robert A. Kennamer | Savannah River Ecology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Curriculum Vitae Faculty & Scientists SREL Home Robert A. Kennamer Savannah River Ecology Laboratory P O Drawer E, Aiken, SC 29802 (803) 725-0387 office (803) 725-3309 fax...

  20. Robert J Goldston | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robert J Goldston Professor of Astrophysical Sciences. former Director, PPPL Robert Goldston is a professor of Astrophysical Sciences at Princeton University and an international leader in the fields of plasma physics and magnetic fusion energy. From 1997 to 2009 he served as Director of PPPL. He is the author of 220 papers in journals and conference proceedings, and in 1995 co- authored with Paul Rutherford the textbook "Introduction to Plasma Physics." He is a contributing author to

  1. Robert G. Green (Acting) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    G. Green (Acting) About Us Robert G. Green (Acting) - Principal Deputy CIO for Enterprise Information Resources Management | Deputy CIO for Architecture Engineering, Technology & Innovation Mr. Robert Green serves as (Acting) Principal Deputy for Enterprise Information Resources Management, focusing on enterprise information management and technology, to include strategy, policy and standards and cyber coordination across the extended DOE enterprise. Prior to his role as Principal Deputy,

  2. Robert L. Fleischer, P. Buford Price, Robert M. Walker, 1971 | U.S. DOE

    Office of Science (SC) Website

    Office of Science (SC) Robert L. Fleischer, P. Buford Price, Robert M. Walker, 1971 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1970's Robert L. Fleischer, P.

  3. Richard Schrock, Robert Grubbs, and Metathesis Method in Organic Synthesis

    Office of Scientific and Technical Information (OSTI)

    Richard Schrock, Robert Grubbs, and Metathesis Method in Organic Synthesis Resources with Additional Information Richard R. Schrock of the Massachusetts Institute of Technology and Robert H. Grubbs of the California Institute of Technology were awarded the 2005 Nobel Prize in Chemistry "for the development of the metathesis method in organic synthesis". Richard R. Schrock Richard R. Schrock Copyright L. Barry Hetherington Robert H. Grubbs Robert H. Grubbs Courtesy California Institute

  4. Robert M. Blue President Dominion Virginia Power

    Broader source: Energy.gov (indexed) [DOE]

    Robert M. Blue President Dominion Virginia Power "Enhancing Resilience in Energy Infrastructure and Addressing Vulnerabilities" Quadrennial Energy Review Task Force April 11, 2014 Good morning Secretary Moniz, Dr. Holdren and members of the Quadrennial Energy Review Task Force. I am Bob Blue, President of Dominion Virginia Power, an operating segment of Dominion Resources serving 2.5 million customers in Virginia and North Carolina. Along with our electric utility, Dominion Resources

  5. Alvin A. Taylor Director Robert D. Perry

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Alvin A. Taylor Director Robert D. Perry Director, Office of Environmental Programs South Carolina Department of Natural Resources 1000 Assembly Street Suite 336 PO Box 167 Columbia, SC 29202 803.734.4199 Office 803.734.9809 Fax vejdaniv@dnr.sc.gov September 27, 2013 Julie A. Smith Office of Electricity Delivery and Energy Reliability Mail Code: OE-20 U.S. Department of Energy 1000 Independence Avenue SW. Washington, DC 20585 REF: Request for Information (RFI) Improving Performance of Federal

  6. Mr. Robert Tate Assistant Secretary Cyclops Corporation

    Office of Legacy Management (LM)

    E-23 Mr. Robert Tate Assistant Secretary Cyclops Corporation 650 Washington Road Pittsburgh, Pennsylvania 15228 Dear Mr. Tate: As you discussed with Gale Turf of my staff, the Cyclops Corporation, Titusville Plant fn Alfquippa, Pennsylvania, has been authorfzed for remedial action. This action will be conducted under the Department of Energy's Formerly Utilized Sites Remedial Action Program. The Department will consult with the Cyclops Corporation before taking any action. I understand that

  7. Robert T. Ciochon (Acting) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    T. Ciochon (Acting) About Us Robert T. Ciochon (Acting) - Deputy CIO for Cybersecurity Rob is the Director of Incident Management for the CIO in the Office of Cybersecurity, IM-32. He is also the Program Manager (PM) for the JC3, the Department's enterprise cybersecurity information sharing and incident management capability. He is working hard to flesh out the JC3 functions and services, through partnerships with DOE Laboratories and Program Offices, and is focusing on improving the

  8. Mr. Robert Muller, Manager General Chemical Corporation

    Office of Legacy Management (LM)

    S 1997 Mr. Robert Muller, Manager General Chemical Corporation 6300 Philadelphia Pike Claymont, Delaware 19703 Dear Mr. Muller: As you may know, the Department of Energy (DOE) is evaluating the radiological condition of sites that were utilized by the Manhattan Engineer District and the Atomic Energy Commission (AEC) during the early years of nuclear development to determine whether they need to be cleaned up and whether the Department has authority to perform such action. Mr. D. T. Murphy of

  9. The Honorable Robert Mort&s,

    Office of Legacy Management (LM)

    , Department of Energy. ,. Wzishington, DC 20585 fJEC 01 1394 ," The Honorable Robert Mort&s, 7501 Meyer Road ~' Spring Frove, Illinois '60081 Dear MayorMortens: , Secretary of Energy Hazel O'i@ari has announced a new approach to bpenness in the Departhent,of Energy (DOE) and its communications with the public. _ In support of,this initiative, we are pleased to forward the enclosed~ information related to the former,Xnternational Register site in your jurisdiction that performed work

  10. Robert B. Laughlin and the Fractional Quantum Hall Effect

    Office of Scientific and Technical Information (OSTI)

    Robert B. Laughlin and the Fractional Quantum Hall Effect Resources with Additional Information Robert B. Laughlin Photo Courtesy of LLNL Robert B. Laughlin shared the 1998 Nobel Prize in Physics with Horst L. Störmer and Daniel C. Tsui for 'their discovery of a new form of quantum fluid with fractionally charged excitations'. ' ... [I]n 1982 ... Störmer and Tsui discovered the effect. In 1983, Laughlin, then at the Lawrence Livermore National Laboratory, provided the theoretical explanation

  11. Robert Curl, Jr. and the Discovery of Fullerenes

    Office of Scientific and Technical Information (OSTI)

    Robert Curl, Jr. and the Discovery of Fullerenes Resources with Additional Information Robert Curl, Jr. Courtesy Tommy LaVergne/ Rice University The 1996 Nobel Prize in Chemistry was awarded to Robert F. Curl, Jr., Richard E. Smalley and Sir Harold Kroto 'for their 1985 discovery of "fullerenes," geometric carbon clusters shaped in the pattern of soccer balls. Though initially a subject of controversy, the discovery of spherical carbon molecules, also called "buckyballs,"

  12. Jefferson Lab Leadership Council - Robert D. McKeown

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Deputy Director for Science,Robert D. McKeown Robert D. McKeown Deputy Director for Science Robert D. McKeown has been the deputy director for science at Jefferson Lab since May 2010. He also serves as a Governor's Distinguished CEBAF Professor at The College of William and Mary. McKeown's research interests include studies of weak interactions in nuclei, neutrino oscillations, parity-violating electron scattering, and the electromagnetic structure of nuclei and nucleons. He first became

  13. Replace Fossil Fuels, Final Technical Report Roberts, William...

    Office of Scientific and Technical Information (OSTI)

    Crude Glycerol as Cost-Effective Fuel for Combined Heat and Power to Replace Fossil Fuels, Final Technical Report Roberts, William L 09 BIOMASS FUELS biofuels, glycerin, glycerol,...

  14. VBH-0060- In the Matter of Robert Burd

    Broader source: Energy.gov [DOE]

    This Decision addresses the complaint filed by Robert Burd (Complainant) against his former employer, Mason and Hangar Corporation (the employer), pursuant to the Department of Energy (DOE)...

  15. Oil Shale and Oil Sands Development Robert Keiter; John Ruple...

    Office of Scientific and Technical Information (OSTI)

    Conjunctive Surface and Groundwater Management in Utah: Implications for Oil Shale and Oil Sands Development Robert Keiter; John Ruple; Heather Tanana; Rebecca Holt 29 ENERGY...

  16. Richard Schrock, Robert Grubbs, and Metathesis Method in Organic...

    Office of Scientific and Technical Information (OSTI)

    Information Richard R. Schrock of the Massachusetts Institute of Technology and Robert H. Grubbs of the California Institute of Technology were awarded the 2005 Nobel Prize in...

  17. Motion of Robert G. Burnley, Director the Commonwealth of Virginia...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Robert G. Burnley, Director the Commonwealth of Virginia Department of Environmental Quality to Deny the District of Columbia Public Service Commission's Petition Further...

  18. ORAU Selects Roberts and Toohey for Environmental Assessment...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    radiological safety manager, radiological engineer and health physicist and in other radiology and health physics related roles at various institutions across the country. Roberts...

  19. Roberts County, South Dakota: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Country Ethanol LLC Places in Roberts County, South Dakota Claire City, South Dakota Corona, South Dakota New Effington, South Dakota Ortley, South Dakota Peever, South Dakota...

  20. Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biology @WIPP Life Begins at 250,000,000 Years WIPP's underground isn't just suited for physics experiments aiming to unlock the mysteries of the Universe, it is also a perfect "dig site" for biologists wanting to chronicle the history of life. 250 million years ago, the area around WIPP was all part of the Permian Sea. Today, the salt beds that make up the WIPP underground provide a time capsule, of sorts, to this ancient era. Researchers have uncovered ancient bacteria, cellulose and

  1. Robert Perry | Inventors | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    new window) Click to share on Tumblr (Opens in new window) "Chemistry is evolving into a multidisciplinary area encompassing biology, material science and physics-and that's...

  2. VWA-0036- In the Matter of Robert Gardner

    Broader source: Energy.gov [DOE]

    This Decision involves a complaint filed by Robert Gardner (Gardner or “Complainant”) under the Department of Energy (DOE) Contractor Employee Protection Program, codified at 10 C.F.R. Part 708....

  3. Robert Curl, Jr. and the Discovery of Fullerenes

    Office of Scientific and Technical Information (OSTI)

    Tommy LaVergne Rice University The 1996 Nobel Prize in Chemistry was awarded to Robert F. Curl, Jr., Richard E. Smalley and Sir Harold Kroto 'for their 1985 discovery of...

  4. Summer 2010 Intern Project- Christopher Roberts | Center for Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Efficient Materials Christopher Roberts POLARIZATION SWITCHING ON InGaN LED Christopher Roberts Physics UC Santa Barbara Mentors: Qimin Yan, Maosheng Miao Faculty Advisor: Chris Van de Walle Department: Materials Science The polarization of light is an important aspect of many lighting and display technologies. Due to the anisotrophic nature of the wurtzite crystal structure, light emitted from InGaN/GaN quantum wells may switch polarization with increasing indium composition, a property

  5. Motion of Robert G. Burnley, Director the Commonwealth of Virginia

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Environmental Quality to Deny the District of Columbia Public Service Commission's Petition Further Analysis of Environmental Impacts of Requested Relief | Department of Energy of Robert G. Burnley, Director the Commonwealth of Virginia Department of Environmental Quality to Deny the District of Columbia Public Service Commission's Petition Further Analysis of Environmental Impacts of Requested Relief Motion of Robert G. Burnley, Director the Commonwealth of Virginia Department

  6. Unlocking the Mysteries of the J. Robert Oppenheimer Transcript |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Unlocking the Mysteries of the J. Robert Oppenheimer Transcript Unlocking the Mysteries of the J. Robert Oppenheimer Transcript October 3, 2014 - 2:48pm Addthis This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the redacted Oppenheimer transcript. | Photo courtesy of the Energy Department. This is an excerpt from the original press release from the Atomic Energy Commission announcing the publication of the

  7. "Ask Argonne" - Robert Jacob, Climate Scientist, Part 2

    ScienceCinema (OSTI)

    Jacob, Robert

    2014-11-24

    Previously, climate scientist Robert Jacob talked a bit about the work he does and invited questions from the public during Part 1 of his "Ask Argonne" video set (http://bit.ly/1aK6WDv). In Part 2, he answers some of the questions that were submitted.

  8. EA-1902: Northern Wind Project, Roberts County, South Dakota

    Broader source: Energy.gov [DOE]

    DOE’s Western Area Power Administration is preparing an EA that evaluates the potential environmental impacts of the proposed Northern Wind Project in Summit, Roberts County, South Dakota. Additional information is available on the project webpage, http://www.wapa.gov/ugp/Environment/NorthernWindFarm.htm.

  9. Academic Year 2009-2010 Intern Project - Christopher Roberts | Center for

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Efficient Materials Christopher Roberts QUANTUM SIMULATIONS OF NITRIDE-BASED LEDS Christopher Roberts Physics UC Santa Barbara Mentor: Dr. Maosheng Miao Faculty Advisor: Chris Van de Walle Department: Materials

  10. Manhattan Project: Leslie Groves and J. Robert Oppenheimer

    Office of Scientific and Technical Information (OSTI)

    Leslie Groves and J. Robert Oppenheimer Home Events > Difficult Choices, 1942 > Groves and the MED, 1942 Events > Bringing it All Together, 1942-1945 Events > Bringing it All Together, 1942-1945 > Implosion Becomes A Necessity, Los Alamos: Laboratory, 1944 Events > Dawn of the Atomic Era, 1945 > Evaluations of Trinity, July 1945 Science > In the Laboratory > Los Alamos Organization and Management, Los Alamos, New Mexico, 1942-1946 Resources > Photo Gallery Leslie

  11. The Honorable Robert E. Williams 44 W. Washington Street

    Office of Legacy Management (LM)

    SEC ; : ' ,-<- I ;;;s The Honorable Robert E. Williams 44 W. Washington Street Shelbyville, Indiana 46176 Dear Mayor Williams: Secretary of Energy Hazel O'Leary has announced a new approach to openness in the Department of Energy (DOE) and its communications with the public. In support of this initiative, we are pleased to forward the enclosed information related to the former General Electric Co. site in your jurisdiction that performed work for DOE or its predecessor agencies. This

  12. USCA Convocation Center 375 Robert Bell Parkway, Aiken, SC 29801

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0, 2014 USCA Convocation Center 375 Robert Bell Parkway, Aiken, SC 29801  Cash Bar Reception Downstairs: 6:00-6:45 pm  Banquet/Lecture: 7:00 pm  Tickets: $50 per person Must be purchased and paid for in advance DEADLINE for reservations: October 3, 2014 Please make checks payable to: CNTA We are a 501 (c)(3) non-profit organization Tax ID #: 57-0953103 Contact CNTA: (803) 649-3456 or email, cnta@bellsouth.net Tickets will be mailed to the address we have on record or as instructed.

  13. WBA-15-0001 - In the Matter of Robert J. Schumacher | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 - In the Matter of Robert J. Schumacher WBA-15-0001 - In the Matter of Robert J. Schumacher On May 29, 2015, OHA granted an Appeal involving a complaint filed by Robert J. Schumacher (Schumacher) against Bechtel National, Inc. (Bechtel), a DOE prime contractor that coordinates the construction of DOE's Hanford Site's Waste Treatment Plant, under the DOE's Contractor Employee Protection Program and its governing regulations set forth at 10 CFR Part 708. Schumacher's complaint was dismissed by

  14. Dr Robert J Stack | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Robert J Stack Chemical Sciences, Geosciences, & Biosciences (CSGB) Division CSGB Home About Staff What's New Research Areas Reports and Activities Science Highlights Principal Investigators' Meetings BES Home Staff Dr. Robert J Stack Print Text Size: A A A FeedbackShare Page Dr. Robert J. Stack Program Manager Physical Biosciences Office of Basic Energy Sciences SC-22.1/Germantown Building U.S. Department of Energy 1000 Independence Avenue, SW Washington, D.C. 20585-1290

  15. Women @ Energy: Laura Riihimaki

    Broader source: Energy.gov [DOE]

    "Beware of the glass ceiling that’s in your own head. Social pressure can make you think you shouldn’t be there, but you can choose to ignore that voice in your own mind. Focus on what you want to do rather than what you think you’re able to do. Don’t be afraid to ask questions, being willing to say you don’t know something in order to learn it may actually be the smartest thing you can do."

  16. Laura Berzak Hopkins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FAQs Visit Us Science Stockpile Stewardship National Security National Competitiveness Fusion and Ignition Experiments Fast Ignition Energy for the Future How to Make a Star How...

  17. Robert Hofstadter, Electron Scattering, the Structure of the...

    Office of Scientific and Technical Information (OSTI)

    ever since that initial discovery, and has been an important factor in all branches of nuclear and high energy physics, in astrophysics, as well as in medicine, biology,...

  18. FIA-15-0051 - In the Matter of Robert H. Leyse | Department of Energy

    Office of Environmental Management (EM)

    1 - In the Matter of Robert H. Leyse FIA-15-0051 - In the Matter of Robert H. Leyse On September 25, 2015, OHA denied in part and remanded in part a FOIA Appeal filed by Robert H. Leyse (Appellant) from a determination issued to him by the DOE's Idaho Operations Office (IOO). In the Appeal, the Appellant challenged the adequacy of the DOE's search for responsive documents. OHA found that IOO had conducted a search reasonably calculated to uncover relevant documents. However, OHA also found that

  19. FIA-11-0018 - In the Matter of Robert M. Balick | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FIA-11-0018 - In the Matter of Robert M. Balick FIA-11-0018 - In the Matter of Robert M. Balick The Department of Energy's (DOE) Office of Hearings and Appeals (OHA) denied a Freedom of Information Act (FOIA) Appeal filed by Robert M. Balick (Appellant). The DOE's Office of Information Resources (OIR) denied the Appellant's request for a fee waiver, claiming that the Appellant had not indicated how he would disseminate the information to the public. OHA found that the Appellant had not included

  20. FIA-11-0018 - In the Matter of Robert M. Balick | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1-0018 - In the Matter of Robert M. Balick FIA-11-0018 - In the Matter of Robert M. Balick On January 12, 2012, the Department of Energy's (DOE) Office of Hearings and Appeals (OHA) denied a Freedom of Information Act (FOIA) Appeal filed by Robert M. Balick (Appellant). The DOE's Office of Information Resources (OIR) denied the Appellant's request for a fee waiver, claiming that the Appellant had not indicated how he would disseminate the information to the public. OHA found that the Appellant

  1. Genomics of wood-degrading fungi Ohm, Robin A.; Riley, Robert...

    Office of Scientific and Technical Information (OSTI)

    Genomics of wood-degrading fungi Ohm, Robin A.; Riley, Robert; Salamov, Asaf; Min, Byoungnam; Choi, In-Geol; Grigoriev, Igor V. Not Available Elsevier None USDOE United States...

  2. U.S. Virgin Islands Wind Resources Update 2014 Roberts, J. O...

    Office of Scientific and Technical Information (OSTI)

    Virgin Islands Wind Resources Update 2014 Roberts, J. O.; Warren, A. 17 WIND ENERGY; 29 ENERGY PLANNING, POLICY AND ECONOMY; 24 POWER TRANSMISSION AND DISTRIBUTION U.S. VIRGIN...

  3. Larson,Cheryl A * PS6 From: Roberts,Timothy C - PGL-5

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Larson,Cheryl A * PS6 From: Roberts,Timothy C - PGL-5 Sent: Wednesday, October 22, 2008 3:29 PM To: Larson,Cheryl A - PS-6; Barham,Theodore J - PGL-5 Subject: FW: Slice Contract...

  4. FIA-12-0070 - In the Matter of Robert Huber | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    70 - In the Matter of Robert Huber FIA-12-0070 - In the Matter of Robert Huber On November 14, 2012, the Office of Hearings and Appeals (OHA) issued a decision denying an appeal (Appeal) from a Freedom of Information Act (FOIA) determination issued by the Idaho Operations Office (IOO). The Appellant appealed the IOO's decision not to release the requested records. The IOO stated that the requested records were the property of its contractor, Battelle Energy Alliance, LLC (BEA), as they were

  5. DOE - Office of Legacy Management -- Roberts and Manders Corp - PA 28

    Office of Legacy Management (LM)

    Roberts and Manders Corp - PA 28 FUSRAP Considered Sites Site: ROBERTS AND MANDERS CORP. (PA.28 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Hatboro , Pennsylvania PA.28-1 Evaluation Year: 1987 PA.28-2 Site Operations: Research/Development operation. Company was considered a candidate for work with beryllium metal - but rejected the opportunity. PA.28-2 PA.28-3 Site Disposition: Eliminated - No indication that radioactive

  6. Robert B. Laughlin, 1984 | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Robert B. Laughlin, 1984 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1980's Robert B. Laughlin, 1984 Print Text Size: A A A FeedbackShare Page Physics: For his

  7. Robert G. Bergman, 1993 | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Robert G. Bergman, 1993 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1990's Robert G. Bergman, 1993 Print Text Size: A A A FeedbackShare Page Chemistry: For

  8. Robert K. Moyzis, 1993 | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Robert K. Moyzis, 1993 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1990's Robert K. Moyzis, 1993 Print Text Size: A A A FeedbackShare Page Life Sciences: For

  9. Robert L. Hellens, 1971 | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Robert L. Hellens, 1971 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1970's Robert L. Hellens, 1971 Print Text Size: A A A FeedbackShare Page Reactors: In recognition

  10. Robert W. Conn, 1984 | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Robert W. Conn, 1984 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1980's Robert W. Conn, 1984 Print Text Size: A A A FeedbackShare Page Nuclear Technology: For his

  11. J. Robert Merriman, 1987 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    J. Robert Merriman, 1987 The Ernest Orlando Lawrence Award Lawrence Award Home Nomination & Selection Guidelines Award Laureates 2010's 2000's 1990's 1980's 1970's 1960's Ceremony The Life of Ernest Orlando Lawrence Contact Information The Ernest Orlando Lawrence Award U.S. Department of Energy SC-2/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-2411 E: Email Us 1980's J. Robert Merriman, 1987 Print Text Size: A A A FeedbackShare Page Nuclear Technology: For

  12. NREL: Energy Analysis - Laura Vimmerstedt

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TP-6A2-44688; EPA 430-R-09-024. Mosey, G.; Heimiller, D.; Dahle, D.; Vimmerstedt, L.; Brady-Sabeff, L. (2007). Converting Limbo Lands to Energy-Generating Stations: Renewable...

  13. Microsoft PowerPoint - Roberts, IV and Stewardship (SSAB April 2010).ppt [Compatibility Mode]

    Office of Environmental Management (EM)

    Independent Verification and Independent Verification and Stewardship April 29, 2010 Sarah Roberts, CHP Acting Program Director, ORISE IEAV Benefits of IV "IV is an important quality assurance step that ensures cleanup goals have been achieved" (DOE Lessons Learned from Independent have been achieved (DOE Lessons Learned from Independent Verification Activities, July 2008) * Offers a cost-effective way to provide assurance that the site was successfully remediated to the risk-based

  14. Sandia's Katrina Groth and Ethan Hecht win inaugural Robert Schefer Best

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Paper award Katrina Groth and Ethan Hecht win inaugural Robert Schefer Best Paper award - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery

  15. Authors: Carol Burns, Mark Chadwick, John Erickson, David Funk, and Robert Fulton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear and Particle Futures (N&PF) Pillar Authors: Carol Burns, Mark Chadwick, John Erickson, David Funk, and Robert Fulton Contributions by: Malcolm Andrews, Mike Baker, Joe Carlson, Bruce Carlsten, Aaron Couture, Mark Crawford, Greg Dale, Ed Dendy, Brenda Dingus, Steve Elliott, Juan Fernandez, Julianna Fessenden-Rahn, Alex Friedland, Chris Fryer, Michael Graesser, Rajan Gupta, Anna Hayes, Andrew Hime, Hui Li, Andi Klein, Tom Kwan, Bob Little, Bill Louis, Christopher Mauger, Pat McGaughey,

  16. Robert McKay recognized for dedicated service | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration Robert McKay recognized for dedicated service | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional Testimony Fact Sheets Newsletters Press Releases Photo Gallery

  17. Robert Schefer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Schefer - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs Advanced Nuclear Energy

  18. ROBERT A

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    JR. 1996. Radiocesium patterns in wood duck eggs and nesting females in a contaminated reservoir. Journal of Wildlife Management 60:186-194. 19. KENNAMER, R.A., C.D. MCCREEDY,...

  19. Pieridae Energy (USA) Ltd. FE Dkt. No. 14-179-LNG | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Comment 129 292015 Laura Kaye Comment 130 292015 Marion Wheeler Comment 131 292015 Phyllis Campbell Comment 132 292015 Warren Ondras Comment 133 292015 Robert Douglas, ...

  20. USCA Convocation Center 375 Robert M. Bell Parkway, Aiken, SC 29801

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1, 2013 USCA Convocation Center 375 Robert M. Bell Parkway, Aiken, SC 29801  Cash Bar Reception 6:00-6:45 pm  Banquet/Lecture 7:00 pm  Tickets: $50 per person Must be purchased and paid for in advance DEADLINE for reservations: September 30, 2013 Please make checks payable to: CNTA We are a 501 ( c ) 3 non-profit organization Tax ID #: 57-0953103 Contact CNTA: (803) 649-3456 or email, cnta@bellsouth.net Tickets will be mailed to the address we have on record or as instructed. Tickets

  1. Finite Cloud Effects at the ACRF TWP Site Patrick Taylor and Robert G. Ellingson

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Finite Cloud Effects at the ACRF TWP Site Patrick Taylor and Robert G. Ellingson Dept. of Meteorology, Florida State University, Tallahassee, FL 32306 Data: Observations are taken at the ACRF TWP Site from June 1999 through May 2003 and obtained from the ARM Data Archive.  These instruments are used to extract cloud field parameters using the frozen turbulence approximation. The cloud parameters are used as input in to the PCLoS Models to determine N e . Summary and Conclusions:  62

  2. CASL-8-2015-0133-000 Robert Salko & Travis Lange Oak Ridge National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3-000 Robert Salko & Travis Lange Oak Ridge National Laboratory Vefa Kucukboyaci & Yixing Sung Westinghouse Electric Co. LLC Scott Palmtag, Core Physics Jess Gehin Oak Ridge National Laboratory Maria Avramova The Pennsylvania State University March 26, 2015 Development of COBRA-TF for Modeling Full-core, Reactor Operating Cycles CASL-U-2015-0133-000 Advances in Nuclear Fuel Management V (ANFM 2015) Hilton Head Island, South Carolina, USA, March 29-April 1, 2015, on CD-ROM, American

  3. U.S. DEPARTMENT OF COMMERCE NATlONAL BUREAU OF STANDARDS Hr. Robert S. Uallclgh

    Office of Legacy Management (LM)

    lpx- . :b"mn;,Cw2' UN &D SdTEi GovERNMEhT . (Ptor. by A.3. i5o-SC) A~emorandzbm - U.S. DEPARTMENT OF COMMERCE NATlONAL BUREAU OF STANDARDS Hr. Robert S. Uallclgh To : AsrocIated Director AdmInIstration DATE: Harch 22, 1968 Through: Dr. Uayne W. Welnkc ChaInan, Radiation Safety Comlttee In regy refer to: 500.02 FROM : Abraham Schwebcl Chief, Health Physics Section . . SUBJECT: Release of bulldIngs at Van Ness Street after decontamination ! From the early 19201s tII1 1952, the

  4. Title Trip Report: Hydrologic Field Reconnaissance Led by Robert Coache, Water Resources

    National Nuclear Security Administration (NNSA)

    Trip Report: Hydrologic Field Reconnaissance Led by Robert Coache, Water Resources Division, Nevada Department of Natural Resources and Conservation, April 24,1986 Technical Reference Document Author Giampaoli, MaryEllen 1 00991 Document Date ERC Index number 4/24/86 05.09.035 Doc u ment Type Box N u m ber Memo 1684-1 Recipients J. Younker HQS. 880517. 1765 Soonco Applications kitomotional Corporation M86-GEO-MEG-054 ADMIN RECORD # 5. DATE: TO: May 7, 1986 J. Younker ADMINISTRATIVE FROM: M.

  5. Historical Information H.2 Biological Studies

    Office of Legacy Management (LM)

    _-_ . - H.2 Biological Studies (0 \ j ; : : j Book . l Ad Hoc Rulison Review Panel Comments Regarding Re-Entry , and Testing Operations, December 22, 1969 This page intentionally left blank December 22, 1969 M r . Robert E. Miller, Manager Nevada Operations Office U. S. Atomic Energy Commission Post Office Box 14100 Las Vegas, Nevada 89114 Dear M r . Miller: F4cmbers of t h e A d Hoc Rulison Review Panel reconvened on December 22, 1969, t o hear and discuss comments r e s u l t i n g from our

  6. L3:PHI.VCS.P9.02 CTF Validation Robert K. Salko Oak Ridge National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 CTF Validation Robert K. Salko Oak Ridge National Laboratory Taylor Blyth, Chris Dances, Jeffrey Mageganz, Maria Avramova Pennsylvania State University August 20, 2014 CASL-U-2014-0169-000 CASL-U-2014-0169-000 The Pennsylvania State University Department of Mechanical and Nuclear Engineering Reactor Dynamics and Fuel Management Group CTF Validation August 20, 2014 CASL-U-2014-0169-000 Authors: Robert K. Salko 1 Taylor S. Blyth 2 Chris A. Dances 3 Jeffrey W. Magedanz 4 Maria N. Avramova 5 1 Oak

  7. FIA-14-0019- In the Matter of Robert Orr, Jr.

    Broader source: Energy.gov [DOE]

    On April 16, 2014, the Office of Hearings and Appeals (OHA) issued a decision denying an appeal (Appeal) from a Freedom of Information Act (FOIA) determination issued by the Department of Energy’s Oak Ridge Office (ORO). The Appellant, Robert Orr, Jr., contested the adequacy of ORO’s search for responsive documents. The OHA reviewed ORO’s description of its search, explaining that a search was conducted by the Oak Ridge National Laboratory (ORNL) by its legal and contracts groups, and they could not locate any responsive documents. Based on the sufficiency of the search terms applied by ORNL in searching for the documents, the OHA determined that the search conducted was adequate and denied the Appeal.

  8. Biological Safety

    Broader source: Energy.gov [DOE]

    The DOE's Biological Safety Program provides a forum for the exchange of best practices, lessons learned, and guidance in the area of biological safety. This content is supported by the Biosurety Executive Team. The Biosurety Executive Team is a DOE-chartered group. The DOE Office of Worker Safety and Health Policy provides administrative support for this group. The group identifies biological safety-related issues of concern to the DOE and pursues solutions to issues identified.

  9. National Laboratory] Basic Biological Sciences(59) Biological...

    Office of Scientific and Technical Information (OSTI)

    Achievements of structural genomics Terwilliger, Thomas C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science Biological Science Abstract Not...

  10. Biological preconcentrator

    DOE Patents [OSTI]

    Manginell, Ronald P.; Bunker, Bruce C.; Huber, Dale L.

    2008-09-09

    A biological preconcentrator comprises a stimulus-responsive active film on a stimulus-producing microfabricated platform. The active film can comprise a thermally switchable polymer film that can be used to selectively absorb and desorb proteins from a protein mixture. The biological microfabricated platform can comprise a thin membrane suspended on a substrate with an integral resistive heater and/or thermoelectric cooler for thermal switching of the active polymer film disposed on the membrane. The active polymer film can comprise hydrogel-like polymers, such as poly(ethylene oxide) or poly(n-isopropylacrylamide), that are tethered to the membrane. The biological preconcentrator can be fabricated with semiconductor materials and technologies.

  11. Laura K. Wolf | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    K. Wolf Coordinating Writer/Editor Argonne National Laboratory 9700 South Cass Avenue Building 240 - Rm. 4136 Argonne, IL 60439 630-252-1881 wolf@alcf.anl.gov

  12. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    F G H I J K L M N O P Q R S T U V W X Y Z Ferguson, Robert J. (Robert J. Ferguson) - Department of Geoscience, University of Calgary Firoozabadi, Abbas (Abbas Firoozabadi) - ...

  13. FINAL MEETING SUMMARY HANFORD ADVISORY BOARD RIVER AND PLATEAU...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Richard Stout Laura Hanses Sarah McCalmont Robert Suyama Harold Heacock Maynard Plahuta Jean Vanni Steve Hudson Dick Smith Others Paula Call, DOE-RL Madeleine Brown, Ecology...

  14. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cells, viruses), plant or soil samples (USDA quarantines), recombinant DNA, or blood-borne pathogen. Biological Use Authorization The great majority of biological work at...

  15. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Highlights Training Workshops & Summer Schools Summer Students Structural Molecular Biology Illuminating Biological Structures at the Atomic and Molecular Levels Your browser...

  16. Roberts_1988.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  17. Pete Henderson & Robert Pincus. ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cloud (PoC) 0.00 0.05 0.10 0.15 0.20 0 5 10 15 z (km) (days 0.00 :1095.96) IPHOC ARSCL SAM PoC (dimensionless) d i u q i l e c i n i a r l e p u a r g w o n s z (km) d e t a u n...

  18. September 2013 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    Synthesis of Polycyclic Natural Products Tuan Hoang Nguyen (2003) 16 > Preliminary Benchmarking Efforts and MCNP Simulation Results for Homeland Security Robert Hayes (2008) 16 > ...

  19. Biological tracer method

    DOE Patents [OSTI]

    Strong-Gunderson, Janet M. (Ten Mile, TN); Palumbo, Anthony V. (Oak Ridge, TN)

    1998-01-01

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer.

  20. Biological tracer method

    DOE Patents [OSTI]

    Strong-Gunderson, J.M.; Palumbo, A.V.

    1998-09-15

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer. 2 figs.

  1. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M.; McDowell, Andrew F.

    2015-11-24

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  2. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2014-04-15

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  3. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology LANL leads the world in computational finishing of microbial genomes Read caption + In 2013, Los Alamos scientist Richard Sayre and his team genetically modified the organisms to harvest light more efficiently for maximum production. Overview of Research and Highlights Researchers at Los Alamos National Laboratory are using their renowned expertise in genomics, computation, and experimental biology as the foundation of a dynamic systems biology capability. Systems

  4. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2013-02-26

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  5. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology Genomics and Systems Biology Los Alamos scientists perform research in functional genomics and structural genomics, and applications for such work cover diverse fields such as energy, agriculture, and environmental cleanup. Contact Us Babetta Marrone Biofuels Program Manager Email Cheryl Kuske DOE BER Biological System Science Division Program Manager Email Kirsten McCabe Emerging Threats Program Manager Email Rebecca McDonald Bioscience Communications Email "We

  6. Integrated Biological Control

    SciTech Connect (OSTI)

    JOHNSON, A.R.

    2002-09-01

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects; and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (apriori) or in response to existing contamination spread (aposteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and apriori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, aposteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  7. Integrated Biological Control

    SciTech Connect (OSTI)

    JOHNSON, A.R.

    2003-10-09

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects, and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (a priori) or in response to existing contamination spread (a posteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and a priori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, a posteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  8. The history of NATO TNF policy: The role of studies, analysis and exercises conference proceedings. Volume 3: Papers by Gen. Robert C. Richardson III (Ret.)

    SciTech Connect (OSTI)

    Rinne, R.L.

    1994-02-01

    This conference was organized to study and analyze the role of simulation, analysis, modeling, and exercises in the history of NATO policy. The premise was not that the results of past studies will apply to future policy, but rather that understanding what influenced the decision process-and how-would be of value. The structure of the conference was built around discussion panels. The panels were augmented by a series of papers and presentations focusing on particular TNF events, issues, studies, or exercises. The conference proceedings consist of three volumes. Volume 1 contains the conference introduction, agenda, biographical sketches of principal participants, and analytical summary of the presentations and discussion panels. Volume 2 contains a short introduction and the papers and presentations from the conference. This volume contains selected papers by Brig. Gen. Robert C. Richardson III (Ret.).

  9. Biological sample collector

    DOE Patents [OSTI]

    Murphy, Gloria A.

    2010-09-07

    A biological sample collector is adapted to a collect several biological samples in a plurality of filter wells. A biological sample collector may comprise a manifold plate for mounting a filter plate thereon, the filter plate having a plurality of filter wells therein; a hollow slider for engaging and positioning a tube that slides therethrough; and a slide case within which the hollow slider travels to allow the tube to be aligned with a selected filter well of the plurality of filter wells, wherein when the tube is aligned with the selected filter well, the tube is pushed through the hollow slider and into the selected filter well to sealingly engage the selected filter well and to allow the tube to deposit a biological sample onto a filter in the bottom of the selected filter well. The biological sample collector may be portable.

  10. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  11. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  12. Biological Hydrogen Production Workshop

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy's (DOE's) National Renewable Energy Laboratory (NREL) held a Biological Hydrogen Production Workshop on September 24–25, 2013, in Golden, Colorado. The workshop...

  13. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal...

  14. Biological and Environmental Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological and Environmental Research Biological and Environmental Research Understanding how genomic information is translated to functional capabilities, and the roles of Earth's biogeochemical systems so we can predict climate decades or centuries into the future. Get Expertise Cheryl Kuske (505) 665-4800 Email James Bossert (505) 667-3644 Email Manvendra Dubey (505) 665-3128 Email Kim Nitschke (505) 667-1186 Email Phil Jones (505) 667-6387 Email Cathy Wilson (505) 667-0202 Email Conducting

  15. Biological Effects of Ionizing Radiation

    DOE R&D Accomplishments [OSTI]

    Ingram, M.; Mason, W. B.; Whipple, G. H.; Howland, J. W.

    1952-04-07

    This report presents a review of present knowledge and concepts of the biological effects of ionizing radiations. Among the topics discussed are the physical and chemical effects of ionizing radiation on biological systems, morphological and physiological changes observed in biological systems subjected to ionizing radiations, physiological changes in the intact animal, latent changes following exposure of biological systems to ionizing radiations, factors influencing the biological response to ionizing radiation, relative effects of various ionizing radiations, and biological dosimetry.

  16. Green Biologics | Open Energy Information

    Open Energy Info (EERE)

    Biologics Jump to: navigation, search Name: Green Biologics Place: Oxfordshire, United Kingdom Sector: Biomass, Renewable Energy Product: Oxfordshire-based industrial biotech...

  17. Atterbury,Laura M DK-7 From: Ex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    March 09, 2010 1234 PM To, FOIA Subject: FO(A Request i:l ( 11% 1) ON RI I % t t 11 I TIUS 1tt4 :- '' to e'a The following is a New FOJA request: Name: Torn Jacobs No...

  18. 7th Annual Systems Biology Symposium: Systems Biology and Engineering

    SciTech Connect (OSTI)

    Galitski, Timothy P.

    2008-04-01

    Systems biology recognizes the complex multi-scale organization of biological systems, from molecules to ecosystems. The International Symposium on Systems Biology has been hosted by the Institute for Systems Biology in Seattle, Washington, since 2002. The annual two-day event gathers the most influential researchers transforming biology into an integrative discipline investingating complex systems. Engineering and application of new technology is a central element of systems biology. Genome-scale, or very small-scale, biological questions drive the enigneering of new technologies, which enable new modes of experimentation and computational analysis, leading to new biological insights and questions. Concepts and analytical methods in engineering are now finding direct applications in biology. Therefore, the 2008 Symposium, funded in partnership with the Department of Energy, featured global leaders in "Systems Biology and Engineering."

  19. Effects of military-authorized activities on the San Joaquin kit fox (Vulpes velox macrotis) at Camp Roberts Army National Guard Training Site, California

    SciTech Connect (OSTI)

    Berry, W.H.; Standley, W.G.; O`Farrell, T.P.; Kato, T.T.

    1992-10-01

    The effects of military-authorized activities on San Joaquin kit fox (Vulpes velox macrotis) were investigated at Camp Roberts Army National Guard Training Site from 1988 to 1991. Military-authorized activities included military training exercises, facilities maintenance, new construction, controlled burning, livestock grazing, and public-access hunting. Positive effects of the military included habitat preservation, preactivity surveys, and natural resources management practices designed to conserve kit foxes and their habitat. Perceived negative effects such as entrapment in dens, shootings during military exercises, and accidental poisoning were not observed. Foxes were observed in areas being used simultaneously by military units. Authorized activities were known to have caused the deaths of three of 52 radiocollared foxes recovered dead: one became entangled in concertina wire, one was believed shot by a hunter, and one was struck by a vehicle. Entanglement in communication wire may have contributed to the death of another radiocollared fox that was killed by a predator. Approximately 10% of kit fox dens encountered showed evidence of vehicle traffic, but denning sites did not appear to be a limiting factor for kit foxes.

  20. Biological Safety | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Safety Biological Safety The DOE's Biological Safety Program provides a forum for the exchange of best practices, lessons learned, and guidance in the area of biological safety. This content is supported by the Biosurety Executive Team. The Biosurety Executive Team is a DOE-chartered group. The DOE Office of Worker Safety and Health Policy provides administrative support for this group. The group identifies biological safety-related issues of concern to the DOE and pursues solutions

  1. Biological particle identification apparatus

    DOE Patents [OSTI]

    Salzman, Gary C. (Los Alamos, NM); Gregg, Charles T. (Los Alamos, NM); Grace, W. Kevin (Los Alamos, NM); Hiebert, Richard D. (Los Alamos, NM)

    1989-01-01

    An apparatus and method for making multiparameter light scattering measurements from suspensions of biological particles is described. Fourteen of the sixteen Mueller matrix elements describing the particles under investigation can be substantially individually determined as a function of scattering angle and probing radiations wavelength, eight elements simultaneously for each of two apparatus configurations using an apparatus which incluees, in its simplest form, two polarization modulators each operating at a chosen frequency, one polarizer, a source of monochromatic electromagnetic radiation, a detector sensitive to the wavelength of radiation employed, eight phase-sensitive detectors, and appropriate electronics. A database of known biological particle suspensions can be assembled, and unknown samples can be quickly identified once measurements are performed on it according to the teachings of the subject invention, and a comparison is made with the database.

  2. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Mission Our Mission The SSRL Structural Molecular Biology program operates as a integrated resource and has three primary areas (or cores) of technological research and development and scientific focus: Macromolecular Crystallography (MC) Small Angle X-ray Scattering/Diffraction (SAXS) X-ray Absorption Spectroscopy (XAS) Central to the core technological developments in all three of these areas is the development and utilization of improved detectors and instrumentation, especially to be

  3. Report of The Structural Biology Subcommittee of The Biological and

    Office of Science (SC) Website

    Environmental Research Advisory Committee | U.S. DOE Office of Science (SC) Report of The Structural Biology Subcommittee of The Biological and Environmental Research Advisory Committee Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings Members Charges/Reports Current BERAC Charges Archive of BERAC Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Charges/Reports Report of The Structural Biology Subcommittee of

  4. Report of the Structural Biology Subcommittee of the Biological and

    Office of Science (SC) Website

    Environmental Research Advisory Committee | U.S. DOE Office of Science (SC) Report of the Structural Biology Subcommittee of the Biological and Environmental Research Advisory Committee Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings Members Charges/Reports Current BERAC Charges Archive of BERAC Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Charges/Reports Report of the Structural Biology Subcommittee of

  5. Biological Applications of Synchrotron Radiation:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Applications of Synchrotron Radiation: An Evaluation of the State of the Field in 2002 A BioSync Report. Issued by the Structural Biology Synchrotron users Organization, October, 2002. 2 Table of Contents: Introduction .................................................................................................... 3 Abbreviations .................................................................................................. 5 Executive Summary

  6. Abengoa Mojave Final Biological Opinion

    Broader source: Energy.gov [DOE]

    Biological Opinion on Mojave Solar, LLC's Mojave Solar Project, San Bernardino County, California (8-8-11-F-3)

  7. Elements in biological AMS

    SciTech Connect (OSTI)

    Vogel, J.S.; McAninch, J.; Freeman, S.

    1996-08-01

    AMS (Accelerator Mass Spectrometry) provides high detection sensitivity for isotopes whose half-lives are between 10 years and 100 million years. {sup 14}C is the most developed of such isotopes and is used in tracing natural and anthropogenic organic compounds in the Earth`s biosphere. Thirty-three elements in the main periodic table and 17 lanthanides or actinides have long lived isotopes, providing potential tracers for research in elemental biochemistry. Overlap of biologically interesting heavy elements and possible AMS tracers is discussed.

  8. Robert Jacob | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    climate change Telephone (630) 252-2983 E-mail jacob@mcs.anl.gov Publications View Publications Website www.mcs.anl.govjacob Projects Accelerated Climate Modeling for Energy...

  9. USCA Convocation Center 375 Robert ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reception Downstairs: 6:00-6:45 pm BanquetLecture: 7:00 pm Tickets: 50 per person Must be purchased and paid for in advance DEADLINE for reservations: October 3, 2014...

  10. Robert Thresher - Research Fellow | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    wind, wave, tidal and current energy technology. Dr. Thresher provides unparalleled expertise in research, development and commercialization of wind and ocean energy technologies. ...

  11. J. Robert Oppenheimer - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For Students and Kids Hanford Fun Facts Classroom Projects Famous People of Hanford Albert Einstein Enrico Fermi Leslie Groves Franklin Matthias Gilbert Church Crawford...

  12. Biological Conversion of Sugars To Hydrocarbons | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    To Hydrocarbons Biological Conversion of Sugars To Hydrocarbons PDF explaining the biological process of bioenergy PDF icon Biological Conversion of Sugars To Hydrocarbons More...

  13. Plant Vascular Biology 2010

    SciTech Connect (OSTI)

    Ding, Biao

    2014-11-17

    This grant supported the Second International Conference on Plant Vascular Biology (PVB 2010) held July 24-28, 2010 on the campus of Ohio State University, Columbus, Ohio. Biao Ding (Ohio State University; OSU) and David Hannapel (Iowa State University; ISU) served as co-chairs of this conference. Biao Ding served as the local organizer. PVB is defined broadly here to include studies on the biogenesis, structure and function of transport systems in plants, under conditions of normal plant growth and development as well as of plant interactions with pathogens. The transport systems cover broadly the xylem, phloem, plasmodesmata and vascular cell membranes. The PVB concept has emerged in recent years to emphasize the integrative nature of the transport systems and approaches to investigate them.

  14. ionic liquids biological-ly derived from lignin and hemicellulose

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biological-ly derived from lignin and hemicellulose - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste

  15. Influence of Barrier Design on Current Collapse in High Voltage AlGaN/GaN

    Office of Scientific and Technical Information (OSTI)

    HEMTs. (Conference) | SciTech Connect Influence of Barrier Design on Current Collapse in High Voltage AlGaN/GaN HEMTs. Citation Details In-Document Search Title: Influence of Barrier Design on Current Collapse in High Voltage AlGaN/GaN HEMTs. Abstract not provided. Authors: Biedermann, Laura Butler ; Kaplar, Robert James ; Marinella, Matthew ; Zavadil, Kevin Robert ; Atcitty, Stanley ; Sun, Min ; Palacios, Tomas Publication Date: 2012-10-01 OSTI Identifier: 1111316 Report Number(s):

  16. Influence of Barrier Design on Current Collapse in High Voltage AlGaN/GaN

    Office of Scientific and Technical Information (OSTI)

    HEMTs. (Conference) | SciTech Connect Conference: Influence of Barrier Design on Current Collapse in High Voltage AlGaN/GaN HEMTs. Citation Details In-Document Search Title: Influence of Barrier Design on Current Collapse in High Voltage AlGaN/GaN HEMTs. Abstract not provided. Authors: DasGupta, Sandeepan ; Biedermann, Laura Butler ; Kaplar, Robert ; Marinella, Matthew ; Zavadil, Kevin Robert ; Atcitty, Stanley ; Sun, Min ; Palacios, Tomas Publication Date: 2013-02-01 OSTI Identifier:

  17. Systems Biology Knowledgebase (GSC8 Meeting)

    ScienceCinema (OSTI)

    Cottingham, Robert W [ORNL

    2011-04-29

    The Genomic Standards Consortium was formed in September 2005. It is an international, open-membership working body which promotes standardization in the description of genomes and the exchange and integration of genomic data. The 2009 meeting was an activity of a five-year funding "Research Coordination Network" from the National Science Foundation and was organized held at the DOE Joint Genome Institute with organizational support provided by the JGI and by the University of California - San Diego. Robert W. Cottingham of Oak Ridge National Laboratory discusses the DOE KnowledgeBase at the Genomic Standards Consortium's 8th meeting at the DOE JGI in Walnut Creek, Calif. on Sept. 9, 2009.

  18. Historical Information H.2 Biological Studies

    Office of Legacy Management (LM)

    Ad Hoc Rulison Review Panel Comments Regarding Re-Entry, Gas Reservoir Testing and Flaring, November 21, 1969 This page intentionally left blank November 21, 1969 1-lr. Robert E. Miller, Manager Nevada Operations Office , U.. S, Atomic Energy Commission Post Office Box 14100 Las Vegas, Nevada 89114 Dear M r . Miller: Members of t h e Ad Hoc Rulison Review Panel m e t on 20 and 2 1 November, 1969, a t t h e AEC1s San Francisco Operations Office t o review data con- cerning ~ r e d i c t i o n s

  19. Michael Levitt and Computational Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Michael Levitt and Computational Biology Resources with Additional Information * Publications Michael Levitt Courtesy of Linda A. Cicero / Stanford News Service Michael Levitt, PhD, professor of structural biology at the Stanford University School of Medicine, has won the 2013 Nobel Prize in Chemistry. ... Levitt ... shares the ... prize with Martin Karplus ... and Arieh Warshel ... "for the development of multiscale models for complex chemical systems." Levitt's work focuses on

  20. Biological Science | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Science Biological Science The protozoan Plasmodium falciparum gliding through a cell in the gut of a mosquito, its primary host. Although five different species of Plasmodium can cause malaria, Plasmodium falciparum causes the most severe disease. | Photo courtesy of Wikipedia Commons. <a href="http://energy.gov/articles/malaria-researchers-find-weakness-global-killer">Read more</a> The protozoan Plasmodium falciparum gliding through a cell in the gut of a

  1. Autofermentative Biological Hydrogen Production by Cyanobacteria...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Hydrogen Production by Cyanobacteria Presentation by Charles Dismukes, Rutgers University, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at...

  2. Renewable Hydrogen Production from Biological Systems

    Broader source: Energy.gov (indexed) [DOE]

    Hydrogen Production from Biological Systems Matthew Posewitz Colorado School of Mines DOE Biological Hydrogen Production Workshop September 24 th , 2013 H 2 production PSIIPSI...

  3. Systems biology approach to bioremediation

    SciTech Connect (OSTI)

    Chakraborty, R.; Wu, C. H.; Hazen, T. C.

    2012-01-01

    Bioremediation has historically been approached as a ?black box? in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at the molecular, cellular, community, and ecosystem level. The knowledge of these interactions within individual components is fundamental to understanding the dynamics of the ecosystem under investigation. Understanding and modeling functional microbial community structure and stress responses in environments at all levels have tremendous implications for our fundamental understanding of hydrobiogeochemical processes and the potential for making bioremediation breakthroughs and illuminating the ?black box?.

  4. SC e-journals, Biology/Genetics

    Office of Scientific and Technical Information (OSTI)

    Biology/Genetics ACM Transactions on Applied Perception (TAP) ACS Chemical Biology ACS Synthetic Biology Acta Biotheoretica Acta Neuropathologica Advances in Bioinformatics - OAJ Advances in Health Sciences Education Agriculture and Human Values Agroforestry Systems American Journal of Agricultural and Biological Science - OAJ American Journal of Medical Genetics Amino Acids Analyst Analytical and Bioanalytical Chemistry Analytical Biochemistry Anatomical Record, The Anatomy and Embryology

  5. Autofermentative Biological Hydrogen Production by Cyanobacteria |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Autofermentative Biological Hydrogen Production by Cyanobacteria Autofermentative Biological Hydrogen Production by Cyanobacteria Presentation by Charles Dismukes, Rutgers University, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado. PDF icon bio_h2_workshop_dismukes.pdf More Documents & Publications 2013 Biological Hydrogen Production Workshop Summary Report Renewable Hydrogen

  6. Semiconductor Nanocrystals for Biological Imaging

    SciTech Connect (OSTI)

    Fu, Aihua; Gu, Weiwei; Larabell, Carolyn; Alivisatos, A. Paul

    2005-06-28

    Conventional organic fluorophores suffer from poor photo stability, narrow absorption spectra and broad emission feature. Semiconductor nanocrystals, on the other hand, are highly photo-stable with broad absorption spectra and narrow size-tunable emission spectra. Recent advances in the synthesis of these materials have resulted in bright, sensitive, extremely photo-stable and biocompatible semiconductor fluorophores. Commercial availability facilitates their application in a variety of unprecedented biological experiments, including multiplexed cellular imaging, long-term in vitro and in vivo labeling, deep tissue structure mapping and single particle investigation of dynamic cellular processes. Semiconductor nanocrystals are one of the first examples of nanotechnology enabling a new class of biomedical applications.

  7. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2006-01-24

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine, methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

  8. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2010-03-23

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens of the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  9. Rapid classification of biological components

    DOE Patents [OSTI]

    Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

    2010-03-23

    A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

  10. Impact of Radiation Biology on Fundamental Insights in Biology

    DOE R&D Accomplishments [OSTI]

    Setlow, Richard B.

    1982-07-27

    Research supported by OHER [Office of Health and Environmental Research] and its predecessors has as one of its major goals an understanding of the effects of radiation at low doses and dose rates on biological systems, so as to predict their effects on humans. It is not possible to measure such effects directly. They must be predicted from basic knowledge on how radiation affects cellular components such as DNA and membranes and how cells react to such changes. What is the probability of radiation producing human mutations and what are the probabilities of radiation producing cancer? The end results of such studies are radiation exposure standards for workers and for the general population. An extension of these goals is setting standards for exposure to chemicals involved in various energy technologies. This latter problem is much more difficult because chemical dosimetry is a primitive state compared to radiation dosimetry.

  11. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.: Davis, J.C.; Stanker, L.H.

    1993-05-11

    A method is disclosed of quantifying molecules in biological substances, comprising: selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere; preparing a long-lived radioisotope labeled reactive chemical specie; administering the chemical specie to the biological host in doses sufficiently low to avoid significant overt damage to the biological system; allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host; isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources; converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation; and measuring the radioisotope concentration in the material by means of direct isotopic counting.

  12. Synthetic Biology and the U.S. Biotechnology Regulatory System: Challenges and Options

    SciTech Connect (OSTI)

    Carter, Sarah R.; Rodemeyer, Michael; Garfinkel, Michele S.; Friedman, Robert M

    2014-05-01

    Synthetic Biology and the U.S. Biotechnology Regulatory System: Challenges and Options Sarah R. Carter, Ph.D., J. Craig Venter Institute; Michael Rodemeyer, J.D., University of Virginia; Michele S. Garfinkel, Ph.D., EMBO; Robert M. Friedman, Ph.D., J. Craig Venter Institute In recent years, a range of genetic engineering techniques referred to as synthetic biology has significantly expanded the tool kit available to scientists and engineers, providing them with far greater capabilities to engineer organisms than previous techniques allowed. The field of synthetic biology includes the relatively new ability to synthesize long pieces of DNA from chemicals, as well as improved methods for genetic manipulation and design of genetic pathways to achieve more precise control of biological systems. These advances will help usher in a new generation of genetically engineered microbes, plants, and animals. The JCVI Policy Center team, along with researchers at the University of Virginia and EMBO, examined how well the current U.S. regulatory system for genetically engineered products will handle the near-term introduction of organisms engineered using synthetic biology. In particular, the focus was on those organisms intended to be used or grown directly in the environment, outside of a contained facility. The study concludes that the U.S. regulatory agencies have adequate legal authority to address most, but not all, potential environmental, health and safety concerns posed by these organisms. Such near-term products are likely to represent incremental changes rather than a marked departure from previous genetically engineered organisms. However, the study also identified two key challenges for the regulatory system, which are detailed in the report. First, USDAs authority over genetically engineered plants depends on the use of an older engineering technique that is no longer necessary for many applications. The shift to synthetic biology and other newer genetic engineering techniques will leave many engineered plants without any pre-market regulatory review. Second, the number and diversity of engineered microbes for commercial use will increase in the near future, challenging EPAs resources, expertise, and perhaps authority to regulate them. For each of these challenges, the report sets out a series of options, including an analysis of the advantages and disadvantages of each option from a variety of perspectives, for policy makers to consider. Policy responses will depend on the trade-offs chosen among competing considerations. This report, funded by the Department of Energy with additional funds from the Alfred P. Sloan Foundation, is the result of a two-year process that included interviews, commissioned background papers, discussions, and two workshops that sought input from a wide range of experts, including U.S. federal agency regulators, legal and science policy experts, representatives from the biotechnology industry, and non-governmental organizations. This cross-section of views informed this report, but the conclusions are solely those of the authors. An Executive Summary, full Report, and background papers are available at: http://www.jcvi.org/cms/research/projects/synthetic-biology-and-the-us-biotechnology-regulatory-system/overview/

  13. OTEC environmental biological oceanographic program

    SciTech Connect (OSTI)

    Hartwig, E.O.

    1981-07-01

    One of the major goals of the OTEC biological field measurement program is to assess the effect of OTEC operations on the environment. Prior understanding of the natural variability of the tropical oceanic plankton community is the most important method for determining changes due to operation of an OTEC plant. The spatial and temporal patterns of the plankton community in terms of absolute number, biomass and species composition have been investigated at potential OTEC sites. Considerable data exist which document the changes with depth of all three measurements. Diel fluctuations in number and species composition have been studied at one site. While horizontal and seasonal patterns of variability likely exist at all sites, they are subtle and remain somewhat unclear. Attempts are now being made to determine the overall trophic structure of the plankton community at these sites using these data, gut content analysis, and information already in the literature.

  14. Electrostatic thin film chemical and biological sensor

    DOE Patents [OSTI]

    Prelas, Mark A. (Columbia, MO); Ghosh, Tushar K. (Columbia, MO); Tompson, Jr., Robert V. (Columbia, MO); Viswanath, Dabir (Columbia, MO); Loyalka, Sudarshan K. (Columbia, MO)

    2010-01-19

    A chemical and biological agent sensor includes an electrostatic thin film supported by a substrate. The film includes an electrostatic charged surface to attract predetermined biological and chemical agents of interest. A charge collector associated with said electrostatic thin film collects charge associated with surface defects in the electrostatic film induced by the predetermined biological and chemical agents of interest. A preferred sensing system includes a charge based deep level transient spectroscopy system to read out charges from the film and match responses to data sets regarding the agents of interest. A method for sensing biological and chemical agents includes providing a thin sensing film having a predetermined electrostatic charge. The film is exposed to an environment suspected of containing the biological and chemical agents. Quantum surface effects on the film are measured. Biological and/or chemical agents can be detected, identified and quantified based on the measured quantum surface effects.

  15. Biological & Environmental Research Abstracts Database

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Welcome to the Biological and Environmental Research Abstracts Database The U.S. Department of Energy's Office of Biological and Environmental Research (BER) conducts research in the areas of Climate and Environmental Sciences and Biological Systems Science. This database contains abstracts of research projects supported by the program. Work was performed at DOE Laboratories as well as at nearly 300 universities and other research institutions. This is a historical database that includes the

  16. Method for photo-altering a biological system to improve biological effect

    DOE Patents [OSTI]

    Hill, Richard A. (Irvine, CA); Doiron, Daniel R. (Santa Ynez, CA); Crean, David H. (Santa Barbara, CA)

    2000-08-01

    Photodynamic therapy is a new adjunctive therapy for filtration surgery that does not use chemotherapy agents or radiation, but uses pharmacologically-active sensitizing compounds to produce a titratable, localized, transient, post operative avascular conjunctiva. A photosensitizing agent in a biological system is selectively activated by delivering the photosensitive agent to the biological system and laser activating only a spatially selected portion of the delivered photosensitive agent. The activated portion of the photosensitive agent reacts with the biological system to obtain a predetermined biological effect. As a result, an improved spatial disposition and effectuation of the biological effect by the photosensitive agent in the biological system is achieved.

  17. 2013 Biological Hydrogen Production Workshop Summary Report ...

    Broader source: Energy.gov (indexed) [DOE]

    summary report for the 2013 Biological Hydrogen Production Workshop. bioh2workshopfinalreport.pdf More Documents & Publications The Hydrogen Program at NREL: A Brief Overview...

  18. Molecular biology of signal transduction in plants

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    This volume contains abstracts of oral presentations and poster sessions of the 1991 Cold Springs Harbor Meeting entitled Molecular Biology of Signal Transduction in Plants.

  19. Developing Biological Specifications for Fish Friendly Turbines

    Broader source: Energy.gov [DOE]

    This factsheet explains studies conducted in a highly reproducible manner to examine the biological effects to fish exposed to a shear environment in the laboratory.

  20. Robert E. Blankenship | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    complexes attached to TiO2 substrates. Lucille P. Markey Distinguished Professor of Arts and Sciences Bio 32311:: Neutron analysis yields insight into bacteria for solar...

  1. Robert "Guild" Copeland

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    "Guild" Copeland - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs

  2. Robert Blankenship Director Washington University Dewey Holten

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Blankenship Director Washington University Dewey Holten Associate Director Washington University Steering Committee Operations Committee Laboratory Facilities Scientific Advisory Committee Mass Spectrometer Facility Hao Zhang Research Scientist Photobioreactor Facility Chris Sherman Research/Lab Assistant Ultrafast Laser Facility Dariusz Niedzwiedzki Research Scientist Kaslina Love Mosley Manager, Research Administration & Operations Rachel Ruggirello Education & Outreach Coordinator

  3. Robert Hager | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hager Associate Research Physicist, Theory Department Contact Information Phone: +1 609 243 3305 Email: rhager

  4. Robert M. Blue President Dominion Virginia Power

    Broader source: Energy.gov (indexed) [DOE]

    ... to integrate new technologies to improve resiliency ... we will continue to be active participants in their process. ... The prices for natural gas during the Polar Vortex days ...

  5. Radially Cooled Toroidal Field Centerpost --- Inventor Robert...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cooling power. The fabrication advantage results because the invention eliminates the need to "gun-drill" many long narrow holes from the centerpost top to bottom. No.: M-840...

  6. Robert S Sheneman | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    S Sheneman Head, Environmental Services Division Interests Sustainability Contact Information Phone: 609-243-3392 Email: rshenema@pppl.gov Learn More Sustainability...

  7. Robert G. Green (Acting) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    recommendations which were approved by the Department's leadership in May 2014. Mr. Green also served as the (Acting) Associate Chief Information Officer for Energy IT...

  8. Robert Albers, Paul Johnson and Kurt Sickafus

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    His work is also leading to possible development of new diagnostic tools for osteoporosis, and he has contributed to programs that monitor for potential nuclear explosions....

  9. Robert B. Raines | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    B. Raines | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Library Bios Congressional Testimony Fact Sheets Newsletters Press Releases Photo Gallery Jobs Apply for Our Jobs Our Jobs Working at NNSA Blog

  10. NREL: Energy Sciences - Robert C. Tenent

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Electron-Transfer Rate Measurements on Modified and Unmodified Glassy-Carbon Electrodes." Journal of Solid State Electrochemistry (13); pp. 583-590. Svedruzic, D., Blackburn, J.L.,...

  11. Ninth International Workshop on Plant Membrane Biology

    SciTech Connect (OSTI)

    Not Available

    1993-12-31

    This report is a compilation of abstracts from papers which were discussed at a workshop on plant membrane biology. Topics include: plasma membrane ATP-ases; plant-environment interactions, membrane receptors; signal transduction; ion channel physiology; biophysics and molecular biology; vaculor H+ pumps; sugar carriers; membrane transport; and cellular structure and function.

  12. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, K.W.; Vogel, J.S.; Felton, J.S.; Gledhill, B.L.; Davis, J.C.

    1994-12-27

    Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering the chemical specie to the biological host in doses sufficiently low to avoid significant overt damage to the biological system, d. allowing a period of time to elapse sufficient for dissemination and interaction of the chemical specie with the host throughout the biological system of the host, e. isolating a reacted fraction of the biological substance from the host in a manner sufficient to avoid contamination of the substance from extraneous sources, f. converting the fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in the material by means of direct isotopic counting. 5 figures.

  13. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.

    1994-01-01

    Disclosed is a method of quantifying molecules in biological substances comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  14. Method of measurement in biological systems

    DOE Patents [OSTI]

    Turteltaub, Kenneth W.; Vogel, John S.; Felton, James S.; Gledhill, Barton L.; Davis, Jay C.; Stanker, Larry H.

    1993-05-11

    Disclosed is a method of quantifying molecules in biological substances, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biological host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  15. Micro/nanofabricated environments for synthetic biology

    SciTech Connect (OSTI)

    Collier, Pat [ORNL; Simpson, Michael L [ORNL

    2011-01-01

    A better understanding of how confinement, crowding and reduced dimensionality modulate reactivity and reaction dynamics will aid in the rational and systematic discovery of functionality in complex biological systems. Artificial micro- and nanofabricated structures have helped elucidate the effects of nanoscale spatial confinement and segregation on biological behavior, particularly when integrated with microfluidics, through precise control in both space and time of diffusible signals and binding interactions. Examples of nanostructured interfaces for synthetic biology include the development of cell-like compartments for encapsulating biochemical reactions, nanostructured environments for fundamental studies of diffusion, molecular transport and biochemical reaction kinetics, and regulation of biomolecular interactions as functions of micro- and nanofabricated topological constraints.

  16. Visualization/In-situ Analysis Co-Chairs: Laura Monroe, David...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    High Fidelity Analysis Opportunities * Domain science involvement * Ability to explore data * Design for end---- users * Light----weight in resource usage Best Practices *...

  17. To: Laura Barhydt, U.S. Department of Energy From: Amy Shepherd...

    Energy Savers [EERE]

    Attending in person 1 80 Fed. Reg. 20116 (April 14, 2015) Charlie Adams, A.O. Smith Bruce Carnevale, Bradford White Brian Cothroll, A.O. Smith Misty Guard, Bradley ...

  18. Genetics and molecular biology of breast cancer

    SciTech Connect (OSTI)

    King, M.C.; Lippman, M.

    1992-12-31

    This volume contains the abstracts of oral presentations and poster sessions presented at the Cold Springs Harbor Meeting on Cancer Cells, this meeting entitled Genetics and Molecular Biology of Breast Cancer.

  19. Algal Biology Program at Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algal Biology Program at Los Alamos gets a star October 11, 2011 LOS ALAMOS, New Mexico, October 11, 2011-Richard Sayre, one of the nation's top specialists in algae and...

  20. 2013 Biological Hydrogen Production Workshop Summary Report

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and the implications for both in vivo and in vitro activity were discussed. "Self-repair" is often cited as an advantage of biological systems, and some of the mechanisms...

  1. Frontiers of NMR in Molecular Biology

    SciTech Connect (OSTI)

    1999-08-25

    NMR spectroscopy is expanding the horizons of structural biology by determining the structures and describing the dynamics of blobular proteins in aqueous solution, as well as other classes of proteins including membrane proteins and the polypeptides that form the aggregates diagnostic of prion and amyloid diseases. Significant results are also emerging on DNA and RNA oligomers and their complexes with proteins. This meeting focused attention on key structural questions emanating from molecular biology and how NMR spectroscopy can be used to answer them.

  2. Molecular Mechanism of Biological Proton Transport

    SciTech Connect (OSTI)

    Pomes, R.

    1998-09-01

    Proton transport across lipid membranes is a fundamental aspect of biological energy transduction (metabolism). This function is mediated by a Grotthuss mechanism involving proton hopping along hydrogen-bonded networks embedded in membrane-spanning proteins. Using molecular simulations, the authors have explored the structural, dynamic, and thermodynamic properties giving rise to long-range proton translocation in hydrogen-bonded networks involving water molecules, or water wires, which are emerging as ubiquitous H{sup +}-transport devices in biological systems.

  3. Biological Air Emissions Control | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Air Emissions Control Biological Air Emissions Control Innovative Technology Enables Low-Cost, Energy-Efficient Treatment of Industrial Exhaust Streams Air quality standards are becoming more stringent for the U.S. wood products industry. Emissions of volatile organic compounds (VOCs) and hazardous air pollutants (HAPs) (including methanol, formaldehyde, acetylaldehyde, and acrolein) during production of wood products must be tightly controlled. Conventional VOCs and HAPs emission

  4. Contacts for the Office of Administrative Operations | Department of Energy

    Energy Savers [EERE]

    Administrative Operations Contacts for the Office of Administrative Operations John D. (Dan) Bullington, Director of Administrative Operations 202-586-7364 dan.bullington@hq.doe.gov Valerie Mills, Management and Program Analysis Officer 202-586-8533 Valerie.mills@hq.doe.gov Claude Barnes, Management Analyst 202-586-2957 Dorothy Cofield, Computer Technician 202-586-9525 Robert Grady, Computer Technician 202-586-9525 Diane Himes, Administrative Officer 202-586-2941 Laura Ryan, Clerk 202-586-1634

  5. Attendees

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Attendees Attendees Registered Attendees Sean Ahern Oak Ridge National Laboratory William Allcock Argonne National Laboratory Sasha Ames Lawrence Livermore National Laboratory Katie Antypas NERSC Stephen Bailey LBNL Robert Ballance Sandia National Laboratories Nick Balthaserr NERSC Ashley Barker Oak Ridge Leadership Computing Facility Blaise Barney LLNL Christopher Beggio Sandia National Laboratories Wes Bethel LBNL Laura Biven DOE Kristofer Bouchard LBNL Jeff Broughton NERSC Suren Byna LBNL

  6. Detter, John C. [Los Alamos National Laboratory] Basic Biological

    Office of Scientific and Technical Information (OSTI)

    State of the Art for Autonomous Detection Systems using Genomic Sequencing Detter, John C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science...

  7. Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) ...

  8. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production More Documents & Publications Techno-Economic Boundary Analysis of Biological Pathways to ...

  9. Biological and Biomimetic Low-Temperature Routes to Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological and Biomimetic Low-Temperature Routes to Materials for Energy Applications Biological and Biomimetic Low-Temperature Routes to Materials for Energy Applications 2012 DOE...

  10. Biological Conversion of Sugars to Hydrocarbons Technology Pathway...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Conversion of Sugars to Hydrocarbons Technology Pathway This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon ...

  11. UK Biotechnology and Biological Sciences Research Council | Open...

    Open Energy Info (EERE)

    Biotechnology and Biological Sciences Research Council Jump to: navigation, search Name: UK Biotechnology and Biological Sciences Research Council Place: London, United Kingdom...

  12. Detection and treatment of chemical weapons and/or biological...

    Office of Scientific and Technical Information (OSTI)

    Detection and treatment of chemical weapons andor biological pathogens Citation Details In-Document Search Title: Detection and treatment of chemical weapons andor biological...

  13. The Intersection of Physics and Biology

    ScienceCinema (OSTI)

    Liphardt, Jan [University of California, Berkeley, California, United States

    2010-09-01

    In April 1953, Watson and Crick largely defined the program of 20th century biology: obtaining the blueprint of life encoded in the DNA. Fifty years later, in 2003, the sequencing of the human genome was completed. Like any major scientific breakthrough, the sequencing of the human genome raised many more questions than it answered. I'll brief you on some of the big open problems in cell and developmental biology, and I'll explain why approaches, tools, and ideas from the physical sciences are currently reshaping biological research. Super-resolution light microscopies are revealing the intricate spatial organization of cells, single-molecule methods show how molecular machines function, and new probes are clarifying the role of mechanical forces in cell and tissue function. At the same time, Physics stands to gain beautiful new problems in soft condensed matter, quantum mechanics, and non-equilibrium thermodynamics.

  14. The acquisition of dangerous biological materials :

    SciTech Connect (OSTI)

    Aceto, Donato Gonzalo; Astuto-Gribble, Lisa M.; Gaudioso, Jennifer M.

    2007-11-01

    Numerous terrorist organizations have openly expressed interest in producing and deploying biological weapons. However, a limiting factor for many terrorists has been the acquisition of dangerous biological agents, as evidenced by the very few successful instances of biological weapons use compared to the number of documented hoaxes. Biological agents vary greatly in their ability to cause loss of life and economic damage. Some agents, if released properly, can kill many people and cause an extensive number of secondary infections; other agents will sicken only a small number of people for a short period of time. Consequently, several biological agents can potentially be used to perpetrate a bioterrorism attack but few are likely capable of causing a high consequence event. It is crucial, from a US national security perspective, to more deeply understand the likelihood that terrorist organizations can acquire the range of these agents. Few studies have attempted to comprehensively compile the technical information directly relevant to the acquisition of dangerous bacteria, viruses and toxins. In this report, technical fact sheets were assembled for 46 potentially dangerous biological agents. Much of the information was taken from various research sources which could ultimately and significantly expedite and improve bioterrorism threat assessments. By systematically examining a number of specific agent characteristics included in these fact sheets, it may be possible to detect, target, and implement measures to thwart future terrorist acquisition attempts. In addition, the information in these fact sheets may be used as a tool to help laboratories gain a rudimentary understanding of how attractive a method laboratory theft is relative to other potential acquisition modes.

  15. Spectroscopic diagnostics for bacteria in biologic sample

    DOE Patents [OSTI]

    El-Sayed, Mostafa A. (Atlanta, GA); El-Sayed, Ivan H. (Somerville, MA)

    2002-01-01

    A method to analyze and diagnose specific bacteria in a biologic sample using spectroscopy is disclosed. The method includes obtaining the spectra of a biologic sample of a non-infected patient for use as a reference, subtracting the reference from the spectra of an infected sample, and comparing the fingerprint regions of the resulting differential spectrum with reference spectra of bacteria in saline. Using this diagnostic technique, specific bacteria can be identified sooner and without culturing, bacteria-specific antibiotics can be prescribed sooner, resulting in decreased likelihood of antibiotic resistance and an overall reduction of medical costs.

  16. Biology and Medicine Division: Annual report 1986

    SciTech Connect (OSTI)

    Not Available

    1987-04-01

    The Biology and Medicine Division continues to make important contributions in scientific areas in which it has a long-established leadership role. For 50 years the Division has pioneered in the application of radioisotopes and charged particles to biology and medicine. There is a growing emphasis on cellular and molecular applications in the work of all the Division's research groups. The powerful tools of genetic engineering, the use of recombinant products, the analytical application of DNA probes, and the use of restriction fragment length polymorphic DNA are described and proposed for increasing use in the future.

  17. Biological and Environmental Research Advisory Committee

    Office of Science (SC) Website

    March 03-04, 2014 | U.S. DOE Office of Science (SC) March 03-04, 2014 Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings Biological and Environmental Research Advisory Committee March 03-04, 2014 Print Text Size: A A A FeedbackShare Page Agenda .pdf file (21KB) Presentations: Sharlene Weatherwax .pdf

  18. Structural Biology | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Structural Biology Biological and Environmental Research (BER) BER Home About Research Biological Systems Science Division (BSSD) Genomic Science DOE Bioenergy Research Centers Bioimaging Technology DOE Joint Genome Institute Structural Biology Radiochemistry & Imaging Instrumentation Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Climate and Environmental Sciences Division (CESD) Research Abstracts Searchable Archive of BER Highlights External link

  19. Physics Meets Biology (LBNL Summer Lecture Series)

    ScienceCinema (OSTI)

    Chu, Steve [Director, LBNL

    2011-04-28

    Summer Lecture Series 2006: If scientists could take advantage of the awesomely complex and beautiful functioning of biologys natural molecular machines, their potential for application in many disciplines would be incalculable. Nobel Laureate and Director of the Lawrence Berkeley National Laboratory Steve Chu explores Possible solutions to global warming and its consequences.

  20. Modular microfluidic system for biological sample preparation

    DOE Patents [OSTI]

    Rose, Klint A.; Mariella, Jr., Raymond P.; Bailey, Christopher G.; Ness, Kevin Dean

    2015-09-29

    A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter.

  1. Radiological/biological/aerosol removal system

    DOE Patents [OSTI]

    Haslam, Jeffery J

    2015-03-17

    An air filter replacement system for existing buildings, vehicles, arenas, and other enclosed airspaces includes a replacement air filter for replacing a standard air filter. The replacement air filter has dimensions and air flow specifications that allow it to replace the standard air filter. The replacement air filter includes a filter material that removes radiological or biological or aerosol particles.

  2. Apparatus for automated testing of biological specimens

    DOE Patents [OSTI]

    Layne, Scott P. (Los Angeles, CA); Beugelsdijk, Tony J. (Los Alamos, NM)

    1999-01-01

    An apparatus for performing automated testing of infections biological specimens is disclosed. The apparatus comprise a process controller for translating user commands into test instrument suite commands, and a test instrument suite comprising a means to treat the specimen to manifest an observable result, and a detector for measuring the observable result to generate specimen test results.

  3. Composites comprising biologically-synthesized nanomaterials

    DOE Patents [OSTI]

    Curran, Seamus; Dias, Sampath; Blau, Werner; Wang, Jun; Oremland, Ronald S; Baesman, Shaun

    2013-04-30

    The present disclosure describes composite materials containing a polymer material and a nanoscale material dispersed in the polymer material. The nanoscale materials may be biologically synthesized, such as tellurium nanorods synthesized by Bacillus selenitireducens. Composite materials of the present disclosure may have optical limiting properties and find use in optical limiting devices.

  4. Centauri High School Teacher Honored as Colorado Outstanding Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Teacher Centauri High School Teacher Honored as Colorado Outstanding Biology Teacher For more information contact: e:mail: Public Affairs Golden, Colo., May 2, 1997 -- Tracy Swedlund, biology teacher at Centauri High School in LaJara, was selected as Colorado's 1997 Outstanding Biology Teacher and will be recognized by the National Association of Biology Teachers (NABT). NABT honors exemplary biology and life science middle and high school educators nationwide. Criteria include teaching

  5. Biological warfare in the littorals. Final report

    SciTech Connect (OSTI)

    Larsen, R.W.

    1997-05-01

    Biological warfare (BW) has emerged as a significant threat to military operations and is particularly challenging at the operational level of warfare in a littoral environment. There are compelling reasons why an operational commander should be concerned about BW: global proliferation of biotechnology and biological weapons capabilities; suitability of BW for disrupting force projection across the littorals; and the vulnerability of American, allied and coalition forces to BW. The threat of facing an adversary capable and willing to use biological weapons will influence the commander`s application of the operational art across the six operational functions. Degradation of operational tempo, effects of psychological responses among the force, and stress on the organizational structure may challenge the command and control process. Operational intelligence will demand robust integration of technical analysis, intentions and warnings, meteorological information, and medical intelligence. The maneuver and movement processes will be taxed to function effectively when ports and airfields offer such lucrative BW targets. Biological weapons may dictate the location of operational fires assets as well as the make-up of the target lists. Operational logistics assumes great importance in the medical functions, decontamination processes, and troop replacement and unit reconstitution. Operational protection encompasses nearly every aspect of BW defense and will demand a balance between what is necessary and what is possible to protect. As daunting as the challenges appear, the operational-level commander has at his disposal many tools necessary to prepare for biological warfare in the littorals. Ultimately, the commander must convince his force, his allies, and his enemies that the command can fight effectively in a BW environment, on land and sea.

  6. 2011 Archaea: Ecology, Metabolism, & Molecular Biology

    SciTech Connect (OSTI)

    Keneth Stedman

    2011-08-05

    Archaea, one of three major evolutionary lineages of life, are a fascinating and diverse group of microbes with deep roots overlapping those of eukaryotes. The focus of the 'Archaea: Ecology Metabolism & Molecular Biology' GRC conference expands on a number of emerging topics highlighting new paradigms in archaeal metabolism, genome function and systems biology; information processing; evolution and the tree of life; the ecology and diversity of archaea and their viruses. The strength of this conference lies in its ability to couple a field with a rich history in high quality research with new scientific findings in an atmosphere of stimulating exchange. This conference remains an excellent opportunity for younger scientists to interact with world experts in this field.

  7. Monitoring Biological Activity at Geothermal Power Plants

    SciTech Connect (OSTI)

    Peter Pryfogle

    2005-09-01

    The economic impact of microbial growth in geothermal power plants has been estimated to be as high as $500,000 annually for a 100 MWe plant. Many methods are available to monitor biological activity at these facilities; however, very few plants have any on-line monitoring program in place. Metal coupon, selective culturing (MPN), total organic carbon (TOC), adenosine triphosphate (ATP), respirometry, phospholipid fatty acid (PLFA), and denaturing gradient gel electrophoresis (DGGE) characterizations have been conducted using water samples collected from geothermal plants located in California and Utah. In addition, the on-line performance of a commercial electrochemical monitor, the BIoGEORGE?, has been evaluated during extended deployments at geothermal facilities. This report provides a review of these techniques, presents data on their application from laboratory and field studies, and discusses their value in characterizing and monitoring biological activities at geothermal power plants.

  8. 2009 Archaea: Ecology, Metabolism & Molecular Biology GRC

    SciTech Connect (OSTI)

    Dr. Julie Maupin- Furlow

    2009-07-26

    Archaea, one of three major evolutionary lineages of life, are a fascinating and diverse group of microbes with deep roots overlapping those of eukaryotes. The focus of the 'Archaea: Ecology Metabolism & Molecular Biology' GRC conference expands on a number of emerging topics highlighting new paradigms in archaeal metabolism, genome function and systems biology; information processing; evolution and the tree of life; the ecology and diversity of archaea and their viruses; and industrial applications. The strength of this conference lies in its ability to couple a field with a rich history in high quality research with new scientific findings in an atmosphere of stimulating exchange. This conference remains an excellent opportunity for younger scientists to interact with world experts in this field.

  9. Developing Biological Specifications for Fish Friendly Turbines

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Developing Biological Specifications for Fish Friendly Turbines The U.S. Department of Energy's Advanced Hydropower Turbine Sys- tem (AHTS) Program supports the research and development of "envi- ronmentally friendly" turbines, i.e., turbine systems in which environmen- tal attributes, such as entrainment survival for fish, are emphasized. Advanced turbines would be suitable for installation at new hydropower facilities and potentially suitable for replacing aging turbines at existing

  10. Computational Tools to Assess Turbine Biological Performance

    SciTech Connect (OSTI)

    Richmond, Marshall C.; Serkowski, John A.; Rakowski, Cynthia L.; Strickler, Brad; Weisbeck, Molly; Dotson, Curtis L.

    2014-07-24

    Public Utility District No. 2 of Grant County (GCPUD) operates the Priest Rapids Dam (PRD), a hydroelectric facility on the Columbia River in Washington State. The dam contains 10 Kaplan-type turbine units that are now more than 50 years old. Plans are underway to refit these aging turbines with new runners. The Columbia River at PRD is a migratory pathway for several species of juvenile and adult salmonids, so passage of fish through the dam is a major consideration when upgrading the turbines. In this paper, a method for turbine biological performance assessment (BioPA) is demonstrated. Using this method, a suite of biological performance indicators is computed based on simulated data from a CFD model of a proposed turbine design. Each performance indicator is a measure of the probability of exposure to a certain dose of an injury mechanism. Using known relationships between the dose of an injury mechanism and frequency of injury (dose–response) from laboratory or field studies, the likelihood of fish injury for a turbine design can be computed from the performance indicator. By comparing the values of the indicators from proposed designs, the engineer can identify the more-promising alternatives. We present an application of the BioPA method for baseline risk assessment calculations for the existing Kaplan turbines at PRD that will be used as the minimum biological performance that a proposed new design must achieve.

  11. KPFM and PFM of Biological Systems

    SciTech Connect (OSTI)

    Rodriguez, Brian [University College, Dublin; Kalinin, Sergei V [ORNL

    2011-01-01

    Surface potentials and electrostatic interactions in biological systems are a key element of cellular regulation and interaction. Examples include cardiac and muscular activity, voltage-gated ion channels, protein folding and assembly, and electroactive cells and electrotransduction. The coupling between electrical, mechanical, and chemical signals and responses in cellular systems necessitates the development of tools capable of measuring the distribution of charged species, surface potentials, and mechanical responses to applied electrical stimuli and vice versa, ultimately under physiological conditions. In this chapter, applications of voltage-modulated atomic force microscopy (AFM) methods including Kelvin probe force microscopy (KPFM) and piezoresponse force microscopy (PFM) to biological systems are discussed. KPFM is a force-sensitive non-contact or intermittent-contact mode AFM technique that allows electrostatic interactions and surface potentials to be addressed. Beyond long-range electrostatic interactions, the application of bias can lead to a mechanical response, e.g., due to linear piezoelectric coupling in polar biopolymers or via more complex electrotransduction and redox pathways in other biosystems. The use and development of PFM, based on direct electromechanical detection, to biological systems will also be addressed. The similarities and limitations of measuring surface potentials and electromechanical coupling in solution will be outlined.

  12. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Biological Imaging by Soft X-Ray Diffraction Microscopy Print Wednesday, 30 November 2005 00:00 Electron and x-ray...

  13. DOE Issues Request for Information on Biological Hydrogen Production...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Hydrogen Production DOE Issues Request for Information on Biological Hydrogen Production January 23, 2014 - 12:00am Addthis The U.S. Department of Energy's (DOE's) Fuel...

  14. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure The Biological Implications of the PP2A Crystal Structure Print Wednesday, 30 January 2008 00:00 Phosphatases, enzymes...

  15. BioLogical Capital BLC | Open Energy Information

    Open Energy Info (EERE)

    BioLogical Capital BLC Jump to: navigation, search Name: BioLogical Capital (BLC) Place: Denver, Colorado Zip: 80202 Product: Denver Colorado-based group focusing on generating...

  16. Hydro Review: Computational Tools to Assess Turbine Biological Performance

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Hydro Review: Computational Tools to Assess Turbine Biological Performance Hydro Review: Computational Tools to Assess Turbine Biological Performance This review covers the BioPA method used to analyze the biological performance of proposed designs to help ensure the safety of fish passing through the turbines at the Priest Rapids Dam in Grant County, Washington. PDF icon Computational Tools to Assess Turbine Biological Performance More Documents & Publications

  17. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Production | Department of Energy Boundary Analysis of Biological Pathways to Hydrogen Production Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production Report documenting the biological and engineering characteristics of five algal and bacterial hydrogen production systems selected by DOE and NREL for evaluation. PDF icon Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production More Documents & Publications Techno-Economic Boundary Analysis

  18. 2013 Biological Hydrogen Production Workshop Summary Report | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Biological Hydrogen Production Workshop Summary Report 2013 Biological Hydrogen Production Workshop Summary Report November 2013 summary report for the 2013 Biological Hydrogen Production Workshop. PDF icon bio_h2_workshop_final_report.pdf More Documents & Publications The Hydrogen Program at NREL: A Brief Overview Hydrogenases and Barriers for Biotechnological Hydrogen Production Technolog

  19. AN INTEGRATED BIOLOGICAL CONTROL SYSTEM AT HANFORD

    SciTech Connect (OSTI)

    JOHNSON AR; CAUDILL JG; GIDDINGS RF; RODRIGUEZ JM; ROOS RC; WILDE JW

    2010-02-11

    In 1999 an integrated biological control system was instituted at the U.S. Department of Energy's Hanford Site. Successes and changes to the program needed to be communicated to a large and diverse mix of organizations and individuals. Efforts at communication are directed toward the following: Hanford Contractors (Liquid or Tank Waste, Solid Waste, Environmental Restoration, Science and Technology, Site Infrastructure), General Hanford Employees, and Hanford Advisory Board (Native American Tribes, Environmental Groups, Local Citizens, Washington State and Oregon State regulatory agencies). Communication was done through direct interface meetings, individual communication, where appropriate, and broadly sharing program reports. The objectives of the communication efforts was to have the program well coordinated with Hanford contractors, and to have the program understood well enough that all stakeholders would have confidence in the work performed by the program to reduce or elimated spread of radioactive contamination by biotic vectors. Communication of successes and changes to an integrated biological control system instituted in 1999 at the Department of Energy's Hanford Site have required regular interfaces with not only a diverse group of Hanford contractors (i.e., those responsible for liquid or tank waste, solid wastes, environmental restoration, science and technology, and site infrastructure), and general Hanford employees, but also with a consortium of designated stake holders organized as the Hanford Advisory Board (i.e., Native American tribes, various environmental groups, local citizens, Washington state and Oregon regulatory agencies, etc.). Direct interface meetings, individual communication where appropriate, and transparency of the biological control program were the methods and outcome of this effort.

  20. Synchronous Behavior of Two Coupled Biological Neurons

    SciTech Connect (OSTI)

    Elson, R.C.; Selverston, A.I.; Elson, R.C.; Selverston, A.I.; Huerta, R.; Rulkov, N.F.; Rabinovich, M.I.; Abarbanel, H.D.; Selverston, A.I.; Huerta, R.; Abarbanel, H.D.

    1998-12-01

    We report experimental studies of synchronization phenomena in a pair of biological neurons that interact through naturally occurring, electrical coupling. When these neurons generate irregular bursts of spikes, the natural coupling synchronizes slow oscillations of membrane potential, but not the fast spikes. By adding artificial electrical coupling we studied transitions between synchrony and asynchrony in both slow oscillations and fast spikes. We discuss the dynamics of bursting and synchronization in living neurons with distributed functional morphology. {copyright} {ital 1998} {ital The American Physical Society}

  1. A brief history of the Biology Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Complex Most of the "Biology" buildings were constructed in early 1945 and were built as expansion capability for the uranium preparation operations being conducted across the street in Buildings 9202 and 9203. These two buildings were built in 1943 and were being used to convert the refined uranium to the material required as feed or source material for the Y-12 calutrons used to separate the uranium 235 isotope from the more common uranium 238. Within a year of operation, the process

  2. LABORATORY OF NUCLEAR MEDICINE AND RADIATION BIOLOGY

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MEDICINE AND RADIATION BIOLOGY 900 VETERAN AVENUE UNIVERSITY OF CALIFORNIA, LOS ANGELES, CALIFORNIA 90024 AND DEPARTMENT OF RADIOLOGICAL SCIENCES UCLA SCHOOL OF MEDICINE, LOS ANGELES, CALIFORNIA 90024 This manuscript is a contribution to the monograph edited by Daniel S. Berman and Dean Mason, entitled "Clinical Nuclear Cardiology". These studies were supported by Contract #DE-AM03-76-SF00012 between the U.S. Department of Energy and the University of California Prepared for the U.S.

  3. LANSCE | Lujan Center | Biology Preparation Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biology Preparation Laboratory The Lujan Center Biolab offers a variety of capabilities. 1) Biodeuteration Lab (BDL) We run a protein expression lab for perdeuteration of user proteins. We offer full perdeuteration (~99%) using our algal-based media for bacterial growth. We also have M9 minimal media made in D2O for expression of up to ~85% perdeuteration. Users can use our lab in person or mail-in a plasmid for us to express for them. We also have standard protein expression equipment:

  4. Third international congress of plant molecular biology: Molecular biology of plant growth and development

    SciTech Connect (OSTI)

    Hallick, R.B.

    1995-02-01

    The Congress was held October 6-11, 1991 in Tucson with approximately 3000 scientists attending and over 300 oral presentations and 1800 posters. Plant molecular biology is one of the most rapidly developing areas of the biological sciences. Recent advances in the ability to isolate genes, to study their expression, and to create transgenic plants have had a major impact on our understanding of the many fundamental plant processes. In addition, new approaches have been created to improve plants for agricultural purposes. This is a book of presentation and posters from the conference.

  5. Acceptance Criteria Framework for Autonomous Biological Detectors

    SciTech Connect (OSTI)

    Dzenitis, J M

    2006-12-12

    The purpose of this study was to examine a set of user acceptance criteria for autonomous biological detection systems for application in high-traffic, public facilities. The test case for the acceptance criteria was the Autonomous Pathogen Detection System (APDS) operating in high-traffic facilities in New York City (NYC). However, the acceptance criteria were designed to be generally applicable to other biological detection systems in other locations. For such detection systems, ''users'' will include local authorities (e.g., facility operators, public health officials, and law enforcement personnel) and national authorities [including personnel from the Department of Homeland Security (DHS), the BioWatch Program, the Centers for Disease Control and Prevention (CDC), and the Federal Bureau of Investigation (FBI)]. The panel members brought expertise from a broad range of backgrounds to complete this picture. The goals of this document are: (1) To serve as informal guidance for users in considering the benefits and costs of these systems. (2) To serve as informal guidance for developers in understanding the needs of users. In follow-up work, this framework will be used to systematically document the APDS for appropriateness and readiness for use in NYC.

  6. Biological and Environmental Research Network Requirements

    SciTech Connect (OSTI)

    Balaji, V.; Boden, Tom; Cowley, Dave; Dart, Eli; Dattoria, Vince; Desai, Narayan; Egan, Rob; Foster, Ian; Goldstone, Robin; Gregurick, Susan; Houghton, John; Izaurralde, Cesar; Johnston, Bill; Joseph, Renu; Kleese-van Dam, Kerstin; Lipton, Mary; Monga, Inder; Pritchard, Matt; Rotman, Lauren; Strand, Gary; Stuart, Cory; Tatusova, Tatiana; Tierney, Brian; Thomas, Brian; Williams, Dean N.; Zurawski, Jason

    2013-09-01

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy (DOE) Office of Science (SC), the single largest supporter of basic research in the physical sciences in the United States. In support of SC programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet be a highly successful enabler of scientific discovery for over 25 years. In November 2012, ESnet and the Office of Biological and Environmental Research (BER) of the DOE SC organized a review to characterize the networking requirements of the programs funded by the BER program office. Several key findings resulted from the review. Among them: 1) The scale of data sets available to science collaborations continues to increase exponentially. This has broad impact, both on the network and on the computational and storage systems connected to the network. 2) Many science collaborations require assistance to cope with the systems and network engineering challenges inherent in managing the rapid growth in data scale. 3) Several science domains operate distributed facilities that rely on high-performance networking for success. Key examples illustrated in this report include the Earth System Grid Federation (ESGF) and the Systems Biology Knowledgebase (KBase). This report expands on these points, and addresses others as well. The report contains a findings section as well as the text of the case studies discussed at the review.

  7. 2010 Plant Molecular Biology Gordon Research Conference

    SciTech Connect (OSTI)

    Michael Sussman

    2010-07-23

    The Plant Molecular Biology Conference has traditionally covered a breadth of exciting topics and the 2010 conference will continue in that tradition. Emerging concerns about food security have inspired a program with three main themes: (1) genomics, natural variation and breeding to understand adaptation and crop improvement, (2) hormonal cross talk, and (3) plant/microbe interactions. There are also sessions on epigenetics and proteomics/metabolomics. Thus this conference will bring together a range of disciplines, will foster the exchange of ideas and enable participants to learn of the latest developments and ideas in diverse areas of plant biology. The conference provides an excellent opportunity for individuals to discuss their research because additional speakers in each session will be selected from submitted abstracts. There will also be a poster session each day for a two-hour period prior to dinner. In particular, this conference plays a key role in enabling students and postdocs (the next generation of research leaders) to mingle with pioneers in multiple areas of plant science.

  8. ANAEROBIC BIOLOGICAL TREATMENT OF PRODUCED WATER

    SciTech Connect (OSTI)

    John R. Gallagher

    2001-07-31

    During the production of oil and gas, large amounts of water are brought to the surface and must be disposed of in an environmentally sensitive manner. This is an especially difficult problem in offshore production facilities where space is a major constraint. The chief regulatory criterion for produced water is oil and grease. Most facilities have little trouble meeting this criterion using conventional oil-water separation technologies. However, some operations have significant amounts of naphthenic acids in the water that behave as oil and grease but are not well removed by conventional technologies. Aerobic biological treatment of naphthenic acids in simulated-produced water has been demonstrated by others; however, the system was easily overloaded by the large amounts of low-molecular-weight organic acids often found in produced waters. The objective of this research was to determine the ability of an anaerobic biological system to treat these organic acids in a simulated produced water and to examine the potential for biodegradation of the naphthenic acids in the anaerobic environment. A small fixed-film anaerobic biological reactor was constructed and adapted to treat a simulated produced water. The bioreactor was tubular, with a low-density porous glass packing material. The inocula to the reactor was sediment from a produced-water holding pond from a municipal anaerobic digester and two salt-loving methanogenic bacteria. During start-up, the feed to the reactor contained glucose as well as typical produced-water components. When glucose was used, rapid gas production was observed. However, when glucose was eliminated and the major organic component was acetate, little gas was generated. Methane production from acetate may have been inhibited by the high salt concentrations, by sulfide, or because of the lack, despite seeding, of microbes capable of converting acetate to methane. Toluene, a minor component of the produced water (0.1 g/L) was removed in the reactor. Batch tests were conducted to examine naphthenic acid biodegradability under several conditions. The conditions used were seed from the anaerobic reactor, wetland sediments under aerobic and anaerobic conditions, and a sterile control. The naphthenic acid was from a commercial source isolated from Gulf Coast petroleum as was dosed at 2 mg/mL. The incubations were for 30 days at 30 C. The results showed that the naphthenic acids were not biodegraded under anaerobic conditions, but were degraded under aerobic conditions. Despite poor performance of the anaerobic reactor, it remains likely that anaerobic treatment of acetate, toluene, and, potentially, other produced-water components is feasible.

  9. Quantifying evolvability in small biological networks

    SciTech Connect (OSTI)

    Nemenman, Ilya; Mugler, Andrew; Ziv, Etay; Wiggins, Chris H

    2008-01-01

    The authors introduce a quantitative measure of the capacity of a small biological network to evolve. The measure is applied to a stochastic description of the experimental setup of Guet et al. (Science 2002, 296, pp. 1466), treating chemical inducers as functional inputs to biochemical networks and the expression of a reporter gene as the functional output. The authors take an information-theoretic approach, allowing the system to set parameters that optimise signal processing ability, thus enumerating each network's highest-fidelity functions. All networks studied are highly evolvable by the measure, meaning that change in function has little dependence on change in parameters. Moreover, each network's functions are connected by paths in the parameter space along which information is not significantly lowered, meaning a network may continuously change its functionality without completely losing it along the way. This property further underscores the evolvability of the networks.

  10. Recovery from chemical, biological, and radiological incidents :

    SciTech Connect (OSTI)

    Franco, David Oliver; Yang, Lynn I.; Hammer, Ann E.

    2012-06-01

    To restore regional lifeline services and economic activity as quickly as possible after a chemical, biological or radiological incident, emergency planners and managers will need to prioritize critical infrastructure across many sectors for restoration. In parallel, state and local governments will need to identify and implement measures to promote reoccupation and economy recovery in the region. This document provides guidance on predisaster planning for two of the National Disaster Recovery Framework Recovery Support Functions: Infrastructure Systems and Economic Recovery. It identifies key considerations for infrastructure restoration, outlines a process for prioritizing critical infrastructure for restoration, and identifies critical considerations for promoting regional economic recovery following a widearea disaster. Its goal is to equip members of the emergency preparedness community to systematically prioritize critical infrastructure for restoration, and to develop effective economic recovery plans in preparation for a widearea CBR disaster.

  11. Overview of selected molecular biological databases

    SciTech Connect (OSTI)

    Rayl, K.D.; Gaasterland, T.

    1994-11-01

    This paper presents an overview of the purpose, content, and design of a subset of the currently available biological databases, with an emphasis on protein databases. Databases included in this summary are 3D-ALI, Berlin RNA databank, Blocks, DSSP, EMBL Nucleotide Database, EMP, ENZYME, FSSP, GDB, GenBank, HSSP, LiMB, PDB, PIR, PKCDD, ProSite, and SWISS-PROT. The goal is to provide a starting point for researchers who wish to take advantage of the myriad available databases. Rather than providing a complete explanation of each database, we present its content and form by explaining the details of typical entries. Pointers to more complete ``user guides`` are included, along with general information on where to search for a new database.

  12. Biology and Medicine Division annual report, 1985

    SciTech Connect (OSTI)

    Not Available

    1986-04-01

    This book briefly describes the activities of the Biology and Medicine Division of the Lawrence Berkeley Laboratory. During the past year the Donner Pavilion program on the treatment of arteriovenous malformations in the brain has chalked up very significant successes. The disease control rate has been high and objective measures of success using cerebral angiography have been established. The new high resolution positron emitting tomographic imager has been demonstrated to operate successfully. In the Radiation Biophysics program, the availability of higher mass ions up to uranium has allowed us cell and tissue studies in a radiation domain that is entirely new. Using uranium beams, investigators have already made new and exciting findings that are described in the body of the report.

  13. 2010 Diffraction Methods in Structural Biology

    SciTech Connect (OSTI)

    Dr. Ana Gonzalez

    2011-03-10

    Advances in basic methodologies have played a major role in the dramatic progress in macromolecular crystallography over the past decade, both in terms of overall productivity and in the increasing complexity of the systems being successfully tackled. The 2010 Gordon Research Conference on Diffraction Methods in Structural Biology will, as in the past, focus on the most recent developments in methodology, covering all aspects of the process from crystallization to model building and refinement, complemented by examples of structural highlights and complementary methods. Extensive discussion will be encouraged and it is hoped that all attendees will participate by giving oral or poster presentations, the latter using the excellent poster display area available at Bates College. The relatively small size and informal atmosphere of the meeting provides an excellent opportunity for all participants, especially younger scientists, to meet and exchange ideas with leading methods developers.

  14. Plant biology research and training for the 21st century

    SciTech Connect (OSTI)

    Kelly, K.

    1992-01-01

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  15. Plant biology research and training for the 21st century

    SciTech Connect (OSTI)

    Kelly, K.

    1992-12-31

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  16. Modular microfluidic system for biological sample preparation (Patent) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Modular microfluidic system for biological sample preparation Citation Details In-Document Search Title: Modular microfluidic system for biological sample preparation A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis

  17. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Office of Scientific and Technical Information (OSTI)

    (Technical Report) | SciTech Connect Biological Conversion of Sugars to Hydrocarbons Technology Pathway Citation Details In-Document Search Title: Biological Conversion of Sugars to Hydrocarbons Technology Pathway This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot-scale demonstrations at NREL. Technical barriers and key research

  18. DOE Releases Biological Monitoring and Sampling Results Report for the

    Energy Savers [EERE]

    Amchitka, Alaska, Site | Department of Energy Biological Monitoring and Sampling Results Report for the Amchitka, Alaska, Site DOE Releases Biological Monitoring and Sampling Results Report for the Amchitka, Alaska, Site October 28, 2013 - 3:39pm Addthis Contractor, Judy Miller, S.M. Stoller Corporation Public Affairs, (970) 248-6363 jmiller@lm.doe.gov GRAND JUNCTION, Colo. - The U.S. Department of Energy today announced the availability of the Amchitka Island, Alaska, Biological Monitoring

  19. Renewable Hydrogen Production from Biological Systems | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy from Biological Systems Renewable Hydrogen Production from Biological Systems Presentation by Matthew Posewitz, Colorado School of Mines, at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado. PDF icon bio_h2_workshop_posewitz.pdf More Documents & Publications The Hydrogen Program at NREL: A Brief Overview Hydrogen, Fuel Cells and Infrastructure Technologies Program: 2002 Annual Progress Report

  20. EERE Success Story-California: Breakthrough in Algae Biology | Department

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Energy Breakthrough in Algae Biology EERE Success Story-California: Breakthrough in Algae Biology January 31, 2014 - 12:00am Addthis Researchers at the Scripps Institution of Oceanography at University of California, San Diego, have made a significant breakthrough in algal biology with implications for biofuels. Algae typically overproduce lipid oils-energy-storing fat molecules used in biofuel production-when they are starved for nutrients, but this starvation also limits their growth

  1. DOE Issues Request for Information on Biological Hydrogen Production |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Biological Hydrogen Production DOE Issues Request for Information on Biological Hydrogen Production January 23, 2014 - 12:00am Addthis The U.S. Department of Energy's (DOE's) Fuel Cell Technologies Office has issued a request for information (RFI) seeking feedback from interested stakeholders regarding biological hydrogen production research and development (R&D) pathways, barriers, issues, and opportunities for development of technologies that can ultimately produce

  2. Deadline Extended for RFI on Biological Hydrogen Production | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy on Biological Hydrogen Production Deadline Extended for RFI on Biological Hydrogen Production February 26, 2014 - 12:00am Addthis DOE has extended the submission deadline for this Request for Information. Responses must be submitted by 5:00 p.m. Eastern Time on March 14, 2014. The U.S. Department of Energy's (DOE's) Fuel Cell Technologies Office has issued a request for information (RFI) seeking feedback from interested stakeholders regarding biological hydrogen production research

  3. California Valley Solar Ranch Biological Assessment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    California Valley Solar Ranch Biological Assessment California Valley Solar Ranch Biological Assessment Biological Assessment for the California Valley Solar Ranch Project San Luis Obispo County, California High Plains Ranch II, LLC (HPR II), a wholly owned subsidiary of SunPower Corporation, Systems ("SunPower") proposes to construct a 250-megawatt (MW) solar photovoltaic (PV) energy plant, the California Valley Solar Ranch Project (CVSR Project or Project), on a 4,747acre site in

  4. Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Production (2009) | Department of Energy Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) Techno-Economic Boundary Analysis of Biological Pathways to Hydrogen Production (2009) Presentation by Brian James, Strategic Analysis Inc., at the Biological Hydrogen Production Workshop held September 24-25, 2013, at the National Renewable Energy Laboratory in Golden, Colorado. PDF icon bio_h2_workshop_james.pdf More Documents & Publications Technoeconomic

  5. Synthetic biology and crop engineering | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic biology and crop engineering Synthetic biology and crop engineering Breakout Session 2: Frontiers and Horizons Session 2-A: Synthetic Biology and the Promise of Biofuels Jonathan Burbaum, Program Director, Department of Energy, Office of Science, ARPA-E PDF icon b13_burbaum_2-a.pdf More Documents & Publications EIS-0481: Final Programmatic Environmental Impact Statement EIS-0481: Draft Programmatic Environmental Impact Statement EIS-0481: Notice of Intent to Prepare a Programmatic

  6. California: Breakthrough in Algae Biology | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakthrough in Algae Biology California: Breakthrough in Algae Biology January 31, 2014 - 12:00am Addthis Researchers at the Scripps Institution of Oceanography at University of California, San Diego, have made a significant breakthrough in algal biology with implications for biofuels. Algae typically overproduce lipid oils-energy-storing fat molecules used in biofuel production-when they are starved for nutrients, but this starvation also limits their growth and multiplication. The Scripps

  7. Kahuku Wind Power Biological Opinion | Department of Energy

    Office of Environmental Management (EM)

    Kahuku Wind Power Biological Opinion Kahuku Wind Power Biological Opinion Kahuku Wind Power, LLC, Construction of the Kahuku Wind Power Facility in Kahuku, O'ahu, Hawaii PDF icon Kahuku Wind Power Biological Opinion More Documents & Publications EA-1726: Final Environmental Assessment EA-1374: Final Environmental Assessment Wind Turbine Interactions with Birds, Bats, and their Habitats: A Summary of Research Results and Priority Questions

  8. January 2013 Most Viewed Documents for Biology And Medicine ...

    Office of Scientific and Technical Information (OSTI)

    January 2013 Most Viewed Documents for Biology And Medicine Nevada Test Site Radiological Control Manual Radiological Control Managers' Council Nevada Test Site Effects of ionizing ...

  9. Methods for isolation and viability assessment of biological organisms

    DOE Patents [OSTI]

    Letant, Sonia Edith; Baker, Sarah Elyse; Bond, Tiziana; Chang, Allan Shih-Ping

    2015-02-03

    Isolation of biological or chemical organisms can be accomplished using a surface enhanced Raman scattering (SERS) system. The SERS system can be a single or a stacked plurality of photonic crystal membranes with noble-metal lined through pores for flowing analyte potentially containing the biological or chemical organisms. The through pores can be adapted to trap individual biological or chemical organisms and emit SERS spectra, which can then be detected by a detector and further analyzed for viability of the biological or chemical organism.

  10. Functionalized apertures for the detection of chemical and biological materials

    DOE Patents [OSTI]

    Letant, Sonia E. (Livermore, CA); van Buuren, Anthony W. (Livermore, CA); Terminello, Louis J. (Danville, CA); Thelen, Michael P. (Danville, CA); Hope-Weeks, Louisa J. (Brentwood, CA); Hart, Bradley R. (Brentwood, CA)

    2010-12-14

    Disclosed are nanometer to micron scale functionalized apertures constructed on a substrate made of glass, carbon, semiconductors or polymeric materials that allow for the real time detection of biological materials or chemical moieties. Many apertures can exist on one substrate allowing for the simultaneous detection of numerous chemical and biological molecules. One embodiment features a macrocyclic ring attached to cross-linkers, wherein the macrocyclic ring has a biological or chemical probe extending through the aperture. Another embodiment achieves functionalization by attaching chemical or biological anchors directly to the walls of the apertures via cross-linkers.

  11. Biological Conversion of Sugars to Hydrocarbons Technology Pathway...

    Office of Scientific and Technical Information (OSTI)

    case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with...

  12. Particle removal and head loss development in biological filters...

    Office of Scientific and Technical Information (OSTI)

    Overall, biological filtration produced a high-quality water. Although effluent turbidites ... Drinking Water Research Div.) Publication Date: 1993-12-01 OSTI Identifier: 5266966 ...

  13. Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production

    SciTech Connect (OSTI)

    James, B. D.; Baum, G. N.; Perez, J.; Baum, K. N.

    2009-09-01

    Report documenting the biological and engineering characteristics of five algal and bacterial hydrogen production systems selected by DOE and NREL for evaluation.

  14. Genomics and Systems Biology of Tuberculosis (2009 JGI User Meeting)

    ScienceCinema (OSTI)

    Galagan, James

    2011-04-25

    James Galagan from the Broad Institute spoke about the "Genomics and Systems Biology of TB" on March 26, 2009 during the 4th Annual User Meeting

  15. Molecular biology of signal transduction in plants. Abstracts

    SciTech Connect (OSTI)

    Not Available

    1991-12-31

    This volume contains abstracts of oral presentations and poster sessions of the 1991 Cold Springs Harbor Meeting entitled Molecular Biology of Signal Transduction in Plants.

  16. Integration of Hyperspectral Imagery and Biosensors for Biological...

    Office of Scientific and Technical Information (OSTI)

    Biological and Chemical Facility Classification Authors: Munsky, Brian 1 ; Yeager, John D. 1 ; Sellars, Scott 2 ; Mukundan, Harshini 1 ; Nadler, Brett Ross 1 + Show...

  17. DOE Publishes Roadmap for New Biological Research for Energy...

    Office of Science (SC) Website

    DOE Publishes Roadmap for New Biological Research for Energy and Environmental Needs News ... Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW ...

  18. Nanojunction Sensors for the Detection of chemical and Biological...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Detection of chemical and Biological Species DOE Grant Recipients Arizona Technology Enterprises Contact Arizona Technology Enterprises About This Technology Technology Marketing...

  19. Linqu Qinchi Biological Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    City, Shandong Province, China Zip: 262600 Product: Shandong-based developer of a biogas plant that registered as a CDM project. References: Linqu Qinchi Biological Co.,...

  20. Method and apparatus for biological sequence comparison

    DOE Patents [OSTI]

    Marr, Thomas G. (Huntington, NY); Chang, William I-Wei (Huntington, NY)

    1997-01-01

    A method and apparatus for comparing biological sequences from a known source of sequences, with a subject (query) sequence. The apparatus takes as input a set of target similarity levels (such as evolutionary distances in units of PAM), and finds all fragments of known sequences that are similar to the subject sequence at each target similarity level, and are long enough to be statistically significant. The invention device filters out fragments from the known sequences that are too short, or have a lower average similarity to the subject sequence than is required by each target similarity level. The subject sequence is then compared only to the remaining known sequences to find the best matches. The filtering member divides the subject sequence into overlapping blocks, each block being sufficiently large to contain a minimum-length alignment from a known sequence. For each block, the filter member compares the block with every possible short fragment in the known sequences and determines a best match for each comparison. The determined set of short fragment best matches for the block provide an upper threshold on alignment values. Regions of a certain length from the known sequences that have a mean alignment value upper threshold greater than a target unit score are concatenated to form a union. The current block is compared to the union and provides an indication of best local alignment with the subject sequence.

  1. Method and apparatus for biological sequence comparison

    DOE Patents [OSTI]

    Marr, T.G.; Chang, W.I.

    1997-12-23

    A method and apparatus are disclosed for comparing biological sequences from a known source of sequences, with a subject (query) sequence. The apparatus takes as input a set of target similarity levels (such as evolutionary distances in units of PAM), and finds all fragments of known sequences that are similar to the subject sequence at each target similarity level, and are long enough to be statistically significant. The invention device filters out fragments from the known sequences that are too short, or have a lower average similarity to the subject sequence than is required by each target similarity level. The subject sequence is then compared only to the remaining known sequences to find the best matches. The filtering member divides the subject sequence into overlapping blocks, each block being sufficiently large to contain a minimum-length alignment from a known sequence. For each block, the filter member compares the block with every possible short fragment in the known sequences and determines a best match for each comparison. The determined set of short fragment best matches for the block provide an upper threshold on alignment values. Regions of a certain length from the known sequences that have a mean alignment value upper threshold greater than a target unit score are concatenated to form a union. The current block is compared to the union and provides an indication of best local alignment with the subject sequence. 5 figs.

  2. Reversibly immobilized biological materials in monolayer films on electrodes

    DOE Patents [OSTI]

    Weaver, Paul F. (Golden, CO); Frank, Arthur J. (Lakewood, CO)

    1993-01-01

    Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.

  3. Reversibly immobilized biological materials in monolayer films on electrodes

    DOE Patents [OSTI]

    Weaver, P.F.; Frank, A.J.

    1993-05-04

    Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The biological materials may include microbes, proteins, and viruses. The electrode surface may consist of reversibly electroactive materials such as polyvinylferrocene, silicon-linked ferrocene or quinone.

  4. Management of Biological Materials in Wastewater from Research & Development Facilities

    SciTech Connect (OSTI)

    Raney, Elizabeth A.; Moon, Thomas W.; Ballinger, Marcel Y.

    2011-04-01

    PNNL has developed and instituted a systematic approach to managing work with biological material that begins in the project planning phase and carries through implementation to waste disposal. This paper describes two major processes used at PNNL to analyze and mitigate the hazards associated with working with biological materials and evaluate them for disposal to the sewer, ground, or surface water in a manner that protects human health and the environment. The first of these processes is the Biological Work Permit which is used to identify requirements for handling, storing, and working with biological materials and the second is the Sewer Approval process which is used to evaluate discharges of wastewaters containing biological materials to assure they meet industrial wastewater permits and other environmental regulations and requirements.

  5. Systems Biology Knowledgebase for a New Era in Biology A Genomics:GTL Report from the May 2008 Workshop

    SciTech Connect (OSTI)

    Gregurick, S.; Fredrickson, J. K.; Stevens, R.

    2009-03-01

    Biology has entered a systems-science era with the goal to establish a predictive understanding of the mechanisms of cellular function and the interactions of biological systems with their environment and with each other. Vast amounts of data on the composition, physiology, and function of complex biological systems and their natural environments are emerging from new analytical technologies. Effectively exploiting these data requires developing a new generation of capabilities for analyzing and managing the information. By revealing the core principles and processes conserved in collective genomes across all biology and by enabling insights into the interplay between an organism's genotype and its environment, systems biology will allow scientific breakthroughs in our ability to project behaviors of natural systems and to manipulate and engineer managed systems. These breakthroughs will benefit Department of Energy (DOE) missions in energy security, climate protection, and environmental remediation.

  6. Biologically based multistage modeling of radiation effects

    SciTech Connect (OSTI)

    William Hazelton; Suresh Moolgavkar; E. Georg Luebeck

    2005-08-30

    This past year we have made substantial progress in modeling the contribution of homeostatic regulation to low-dose radiation effects and carcinogenesis. We have worked to refine and apply our multistage carcinogenesis models to explicitly incorporate cell cycle states, simple and complex damage, checkpoint delay, slow and fast repair, differentiation, and apoptosis to study the effects of low-dose ionizing radiation in mouse intestinal crypts, as well as in other tissues. We have one paper accepted for publication in ''Advances in Space Research'', and another manuscript in preparation describing this work. I also wrote a chapter describing our combined cell-cycle and multistage carcinogenesis model that will be published in a book on stochastic carcinogenesis models edited by Wei-Yuan Tan. In addition, we organized and held a workshop on ''Biologically Based Modeling of Human Health Effects of Low dose Ionizing Radiation'', July 28-29, 2005 at Fred Hutchinson Cancer Research Center in Seattle, Washington. We had over 20 participants, including Mary Helen Barcellos-Hoff as keynote speaker, talks by most of the low-dose modelers in the DOE low-dose program, experimentalists including Les Redpath (and Mary Helen), Noelle Metting from DOE, and Tony Brooks. It appears that homeostatic regulation may be central to understanding low-dose radiation phenomena. The primary effects of ionizing radiation (IR) are cell killing, delayed cell cycling, and induction of mutations. However, homeostatic regulation causes cells that are killed or damaged by IR to eventually be replaced. Cells with an initiating mutation may have a replacement advantage, leading to clonal expansion of these initiated cells. Thus we have focused particularly on modeling effects that disturb homeostatic regulation as early steps in the carcinogenic process. There are two primary considerations that support our focus on homeostatic regulation. First, a number of epidemiologic studies using multistage carcinogenesis models that incorporate the ''initiation, promotion, and malignant conversion'' paradigm of carcinogenesis are indicating that promotion of initiated cells is the most important cellular mechanism driving the shape of the age specific hazard for many types of cancer. Second, we have realized that many of the genes that are modified in early stages of the carcinogenic process contribute to one or more of four general cellular pathways that confer a promotional advantage to cells when these pathways are disrupted.

  7. Kinetic model for anaerobic digestion of biogas biological sludge

    SciTech Connect (OSTI)

    Pavlostathis, S.G.; Gossett, J.M.

    1986-10-01

    The principal objective of this study was the development and evaluation of a comprehensive kinetic model capable of predicting digester performance when fed biological sludge. Preliminary conversion mechanisms such as cell deaths, lysis, and hydrolysis responsible for rendering viable biological sludge organisms to available substrate were studied in depth. The results of this study indicate that hydrolysis of the dead, particulate biomass - primarily consisting of protein - is the slowest step, and therefore kinetically controls the overall process of an anaerobic digestion of biological sludge. A kinetic model was developed which could accurately describe digester performance and predict effluent quality.

  8. EA-2011: Proposed Release of Three Parasitoids for the Biological...

    Broader source: Energy.gov (indexed) [DOE]

    the continental U.S. for the biological control of the emerald ash borer, a nonnative invasive beetle. The DOE Oak Ridge Office reviewed the EA, adopted it, and issued a FONSI for...

  9. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2014-05-27

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  10. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2013-10-01

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  11. Biological assessments for the low energy demonstration accelerator, 1996

    SciTech Connect (OSTI)

    Cross, S.

    1997-03-01

    This report discusses the biological impact to the area around the Los Alamos National Laboratory of the Low Energy Demonstration Accelerator. In particular the impact to the soils, water quality, vegetation, and wildlife are discussed.

  12. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2014-10-28

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  13. Methods of increasing secretion of polypeptides having biological activity

    DOE Patents [OSTI]

    Merino, Sandra

    2015-04-14

    The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

  14. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in...

  15. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their...

  16. Arms Control: US and International efforts to ban biological weapons

    SciTech Connect (OSTI)

    Not Available

    1992-12-01

    The Bacteriological (Biological) and Toxin Weapons Convention, the treaty that bans the development, production, and stockpiling and acquisition of biological weapons was opened for signature in 1972 and came into force in 1975 after being ratified by 22 governments, including the depository nations of the USA, the United Kingdom, and the former Soviet Union. In support of the Convention, the USA later established export controls on items used to make biological weapons. Further, in accordance with the 1990 President`s Enhanced Proliferation Control Initiative, actions were taken to redefine and expand US export controls, as well as to encourage multilateral controls through the Australia Group. Thus far, the Convention has not been effective in stopping the development of biological weapons. The principal findings as to the reasons of the failures of the Convention are found to be: the Convention lacks universality, compliance measures are effective, advantage of verification may outweigh disadvantages. Recommendations for mitigating these failures are outlined in this report.

  17. Browse by Discipline -- E-print Network Subject Pathways: Biology...

    Office of Scientific and Technical Information (OSTI)

    L M N O P Q R S T U V W X Y Z Keller, Christopher (Christopher Keller) - Department of Biology, Minot State University Go back to Individual Researchers Collections: A B C D E F G ...

  18. Investigations of biological processes in Austrian MBT plants

    SciTech Connect (OSTI)

    Tintner, J.; Smidt, E.; Boehm, K.; Binner, E.

    2010-10-15

    Mechanical biological treatment (MBT) of municipal solid waste (MSW) has become an important technology in waste management during the last decade. The paper compiles investigations of mechanical biological processes in Austrian MBT plants. Samples from all plants representing different stages of degradation were included in this study. The range of the relevant parameters characterizing the materials and their behavior, e.g. total organic carbon, total nitrogen, respiration activity and gas generation sum, was determined. The evolution of total carbon and nitrogen containing compounds was compared and related to process operation. The respiration activity decreases in most of the plants by about 90% of the initial values whereas the ammonium release is still ongoing at the end of the biological treatment. If the biogenic waste fraction is not separated, it favors humification in MBT materials that is not observed to such extent in MSW. The amount of organic carbon is about 15% dry matter at the end of the biological treatment.

  19. Biological and Environmental Research Advisory CommitteeOctober...

    Office of Science (SC) Website

    Geernaert .pdf file (2.1MB) - Climate and Environmental Sciences Division Update Todd Anderson .pdf file (5.1MB) - Biological Systems Science Division Update Kent Peters .pdf file...

  20. Workshop Tackles Biological Hydrogen Production - News Releases | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Workshop Tackles Biological Hydrogen Production Available report outlines goals, challenges, and research areas October 21, 2003 Golden, Colo. - The results of a two-day workshop on hydrogen production through biological and artificial systems now is available. The workshop, sponsored by the Air Force Office of Scientific Research and hosted jointly by the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) and the Air Force Research Laboratory (AFRL), included key

  1. Solar Energy Education. Renewable energy activities for biology (Technical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Report) | SciTech Connect biology Citation Details In-Document Search Title: Solar Energy Education. Renewable energy activities for biology × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy science and technology. A paper copy of this document is also available for sale

  2. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure The Biological Implications of the PP2A Crystal Structure Print Wednesday, 30 January 2008 00:00 Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a

  3. Importance of systems biology in engineering microbes for biofuel

    Office of Scientific and Technical Information (OSTI)

    production (Journal Article) | SciTech Connect Importance of systems biology in engineering microbes for biofuel production Citation Details In-Document Search Title: Importance of systems biology in engineering microbes for biofuel production Microorganisms have been rich sources for natural products, some of which have found use as fuels, commodity chemicals, specialty chemicals, polymers, and drugs, to name a few. The recent interest in production of transportation fuels from renewable

  4. Integration of Hyperspectral Imagery and Biosensors for Biological and

    Office of Scientific and Technical Information (OSTI)

    Chemical Facility Classification (Technical Report) | SciTech Connect Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification Citation Details In-Document Search Title: Integration of Hyperspectral Imagery and Biosensors for Biological and Chemical Facility Classification Authors: Munsky, Brian [1] ; Yeager, John D. [1] ; Sellars, Scott [2] ; Mukundan, Harshini [1] ; Nadler, Brett Ross [1] + Show Author Affiliations Los Alamos National Laboratory UC Irvine

  5. Integration of hyperspectral imagery and biosensors for biological and

    Office of Scientific and Technical Information (OSTI)

    chemical facility classification (Technical Report) | SciTech Connect hyperspectral imagery and biosensors for biological and chemical facility classification Citation Details In-Document Search Title: Integration of hyperspectral imagery and biosensors for biological and chemical facility classification Authors: Munsky, Brian [1] ; Sellars, Scott [2] ; Yeager, John D. [1] ; Mukundan, Harshini [1] ; Nadler, Brett Ross [1] + Show Author Affiliations Los Alamos National Laboratory UC Irvine

  6. A Systems Biology Platform for Characterizing Regulatory and Metabolic

    Office of Scientific and Technical Information (OSTI)

    Pathways that Influence and Control Microbial Hydrogen Production (Technical Report) | SciTech Connect Technical Report: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Citation Details In-Document Search Title: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production × You are accessing a document from the Department of

  7. Comparative systems biology across an evolutionary gradient within the

    Office of Scientific and Technical Information (OSTI)

    Shewanella genus (Journal Article) | SciTech Connect Comparative systems biology across an evolutionary gradient within the Shewanella genus Citation Details In-Document Search Title: Comparative systems biology across an evolutionary gradient within the Shewanella genus To what extent genotypic differences translate to phenotypic variation remains a poorly understood issue of paramount importance for several cornerstone concepts of microbiology such as the species definition. Here, we take

  8. Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced

    Office of Scientific and Technical Information (OSTI)

    Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). (Conference) | SciTech Connect Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Abstract not provided. Authors: Reichmuth, David ; Kozina, Carol L. ; Sale, Kenneth L. ;

  9. A Systems Biology Platform for Characterizing Regulatory and Metabolic

    Office of Scientific and Technical Information (OSTI)

    Pathways that Influence and Control Microbial Hydrogen Production (Technical Report) | SciTech Connect Technical Report: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Citation Details In-Document Search Title: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Authors: Collins, James J [1] + Show Author Affiliations

  10. A comprehensive approach to decipher biological computation to achieve next

    Office of Scientific and Technical Information (OSTI)

    generation high-performance exascale computing. (Technical Report) | SciTech Connect Technical Report: A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. Citation Details In-Document Search Title: A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. The human brain (volume=1200cm3) consumes 20W and is capable of performing>10%5E16 operations/s.

  11. A Systems Biology Platform for Characterizing Regulatory and Metabolic

    Office of Scientific and Technical Information (OSTI)

    Pathways that Influence and Control Microbial Hydrogen Production (Technical Report) | SciTech Connect A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Citation Details In-Document Search Title: A Systems Biology Platform for Characterizing Regulatory and Metabolic Pathways that Influence and Control Microbial Hydrogen Production Authors: Collins, James J [1] + Show Author Affiliations Boston University

  12. A comprehensive approach to decipher biological computation to achieve next

    Office of Scientific and Technical Information (OSTI)

    generation high-performance exascale computing. (Technical Report) | SciTech Connect A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. Citation Details In-Document Search Title: A comprehensive approach to decipher biological computation to achieve next generation high-performance exascale computing. The human brain (volume=1200cm3) consumes 20W and is capable of performing>10%5E16 operations/s. Current

  13. Biological and Environmental Research Advisory Committee (BERAC) Homepage |

    Office of Science (SC) Website

    U.S. DOE Office of Science (SC) BERAC Home Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Print Text Size: A A A FeedbackShare Page The Biological and Environmental Research Advisory Committee (BERAC) provides advice on a continuing basis to the Director of the Office of Science, Department of Energy, on the many complex scientific and

  14. Flow cytometry aids basic cell biology research and drug discovery

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Flow cytometry aids basic cell biology research and drug discovery Flow cytometry aids basic cell biology research and drug discovery Life Technologies Corporation and LANL have released the Attune® Acoustic Focusing Cytometer, featuring a reduced footprint, reduced consumables, and an affordable price. April 3, 2012 Attune® Acoustic Focusing Cytometer The Attune® Acoustic Focusing Cytometer achieves sample throughput at rates over 10 times faster than other cytometers-up to 1,000 μL per

  15. Artificial photosynthesis combines biology with technology for sustainable

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    energy transformation Artificial photosynthesis combines biology with technology for sustainable energy transformation Authors: Moore, T.A., Moore, A.L., and Gust, D. Title: Artificial photosynthesis combines biology with technology for sustainable energy transformation Source: AIP Conf. Proc. Year: 2013 Volume: 1519 Pages: 68-72 ABSTRACT: Photosynthesis supports the biosphere. Currently, human activity appropriates about one fourth of terrestrial photosynthetic net primary production (NPP)

  16. Large Scale Production Computing and Storage Requirements for Biological

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Environmental Research: Target 2017 Large Scale Production Computing and Storage Requirements for Biological and Environmental Research: Target 2017 BERmontage.gif September 11-12, 2012 Hilton Rockville Hotel and Executive Meeting Center 1750 Rockville Pike Rockville, MD, 20852-1699 TEL: 1-301-468-1100 Sponsored by: U.S. Department of Energy Office of Science Office of Advanced Scientific Computing Research (ASCR) Office of Biological and Environmental Research (BER) National Energy

  17. Guidelines to improve airport preparedness against chemical and biological terrorism.

    SciTech Connect (OSTI)

    Edwards, Donna M.; Price, Phillip N. (Lawrence Berkeley National Laboratory, Berkeley, CA); Gordon, Susanna P.; Gadgil, Ashok (Lawrence Berkeley National Laboratory, Berkeley, CA)

    2005-05-01

    Guidelines to Improve Airport Preparedness Against Chemical and Biological Terrorism is a 100-page document that makes concrete recommendations on improving security and assessing vulnerable areas and helps its readers understand the nature of chemical and biological attacks. The report has been turned over to Airports Council International (ACI) and the American Association of Airport Executives (AAAE), two organizations that together represent the interests of thousands of airport personnel and facilities in the U.S. and around the world.

  18. Computational Biology: A Recipe for Ligand-Binding Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computational Biology: A Recipe for Ligand-Binding Proteins Authors: Ghirlanda, G. Title: Computational Biology: A Recipe for Ligand-Binding Proteins Source: Nature Year: 2013 Volume: 501 Pages: 177-178 ABSTRACT: Cellular cross-talk, enzymatic catalysis and regulation of gene expression all depend on molecular recognition. A method that allows the design of proteins with desired recognition sites could thus be revolutionary Date of online publication: Thu, 2013-09-12 Link online:

  19. Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of

    Office of Scientific and Technical Information (OSTI)

    Ethanol from 5-Carbon Sugars (LDRD %23 105944). (Conference) | SciTech Connect Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Citation Details In-Document Search Title: Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of Ethanol from 5-Carbon Sugars (LDRD %23 105944). Abstract not provided. Authors: Sapra, Rajat ; Reichmuth, David ; Kozina, Carol L. ; Sale, Kenneth L. ; Keasling, Jay ; Tang,

  20. Biological Lignin Depolymerization Presentation for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE BioEnergy Technologies Office (BETO) Project Peer Review Date: March 25 th , 2015 Technology Review Area: Biochemical Conversion Biological Lignin Depolymerization (WBS 2.3.2.100) Principal Investigators: Gregg Beckham (NREL) John Gladden (SNL) Organizations: National Renewable Energy Laboratory and Sandia National Laboratory 2 | Bioenergy Technologies Office Project Goal Residual Biorefinery Lignin Goal and Outcome: develop a biological approach to depolymerize solid lignin for upgrading

  1. Environmental Impacts of Wind Power Development on the Population Biology

    Office of Environmental Management (EM)

    of Greater Prairie-Chickens | Department of Energy Environmental Impacts of Wind Power Development on the Population Biology of Greater Prairie-Chickens Environmental Impacts of Wind Power Development on the Population Biology of Greater Prairie-Chickens This report summarizes the results of a seven-year, DOE-funded research project, conducted by researchers from Kansas State University and the National Wind Coordinating Collaborative, to assess the effects of wind energy development in

  2. Behavior of nanoceria in biologically-relevant environments

    SciTech Connect (OSTI)

    Kumar, Amit; Das, Soumen; Munusamy, Prabhakaran; Self, William; Baer, Donald R.; Sayle, Dean C.; Seal, Sudipta

    2014-09-08

    Cerium oxide nanoparticles (CNPs) have gained a considerable attention in biological research due to their anti-oxidant like behaviour and regenerative nature. The current literature on CNPs reports many successful attempts on harnessing the beneficial therapeutic properties in biology. However studies have also shown toxicity effect with some types of CNPs. This review discusses issues associated with the behaviours of CNPs in biological systems and identifies key knowledge gaps. We explore how salient physicochemical properties (size, surface chemistry, surface stabilizers) contribute to the potential positive and negative aspects of nanoceria in biological systems. Based on variations of results reported in the literature, important issues need to be addressed. Are we really studying the same particles with slight variations in size and physicochemical properties or do the particles being examined have fundamentally different behaviours? Are the variations observed in the result of differences in the initial properties of the particles or the results of downstream effects that emerge as the particles are prepared for specific studies and they interact with biological or other environmental moieties? How should particles be appropriately prepared for relevant environmental/toxicology/safety studies? It is useful to recognize that nanoparticles encompass some of the same complexities and variability associated with biological components

  3. Robert McKay recognized for dedicated service | National Nuclear...

    National Nuclear Security Administration (NNSA)

    This Site Budget IG Web Policy Privacy No Fear Act Accessibility FOIA Sitemap Federal Government The White House DOE.gov USA.gov Jobs Apply for Our Jobs Our Jobs Working at NNSA...

  4. Unlocking the Mysteries of the J. Robert Oppenheimer Transcript...

    Broader source: Energy.gov (indexed) [DOE]

    Atomic Energy Commission in 1947 ruled favorably to grant Oppenheimer's clearance. An FBI investigation in 1944 found no reason to revoke his clearance. With the onset of the...

  5. Carbon Cycle 2.0: Robert Cheng and Juan Meza

    ScienceCinema (OSTI)

    Robert Cheng and Juan Meza

    2010-09-01

    Feb. 4, 2010: Humanity emits more carbon into the atmosphere than natural processes are able to remove - an imbalance with negative consequences. Carbon Cycle 2.0 is a Berkeley Lab initiative to provide the science needed to restore this balance by integrating the Labs diverse research activities and delivering creative solutions toward a carbon-neutral energy future.

  6. Scott Robert [ORNL]; Lavrik, Nickolay V [ORNL]; Bannuru, Thirumalesh

    Office of Scientific and Technical Information (OSTI)

    ORNL; Mostafa, Salwa ORNL; Rajic, Slobodan ORNL; Datskos, Panos G ORNL 14 SOLAR ENERGY; COMPUTERS; EFFICIENCY; ELECTRICITY; ENERGY CONVERSION; FABRICATION;...

  7. Robert Hofstadter, Electron Scattering, the Structure of the...

    Office of Scientific and Technical Information (OSTI)

    ... Elastic Electron Scattering from Tritium and Helium-3; DOE Technical Report; 1964 K-edge Subtraction Angiography with Synchrotron X-Rays: Final Technical Report, (February 1, 1984 ...

  8. Microsoft PowerPoint - Roberts, IV and Stewardship (SSAB April...

    Office of Environmental Management (EM)

    an important quality assurance step that ensures cleanup goals have been achieved" (DOE Lessons Learned from Independent have been achieved (DOE Lessons Learned from Independent...

  9. VBX-0060- In the Matter of Robert Burd

    Broader source: Energy.gov [DOE]

    On November 16, 2001, BWXT Pantex, as successor to Mason & Hanger Corporation (M&H) (collectively referred to as “the contractor”), filed an appeal of an Initial Agency Decision (IAD)...

  10. TBB-0040- In the Matter of Caroline Roberts

    Broader source: Energy.gov [DOE]

    This letter concerns the complaint of retaliation that you filed with the Department of Energy (DOE} under 10 C.F.R. Part 708. On March 24, 2006, the Office of Hearings and Appeals (OHA} received...

  11. February 2014 QBR Responses to Robert Kahn, Executive Director

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    expectations over the remainder of the fiscal year. The Treasury Payment Probability (TPP) standard is a rate-making standard for determining whether business unit revenues...

  12. J. Robert Schrieffer and the BCS Theory of Superconductivity

    Office of Scientific and Technical Information (OSTI)

    and his research is available in full-text and on the Web. Documents: Microscopic Theory of Superconductivity; Physical Review, Vol. 106, Issue 1: 162-164, April 1, 1957...

  13. MHK Projects/Robert C Byrd | Open Energy Information

    Open Energy Info (EERE)

    Installed Capacity (MW) 0 Main Overseeing Organization McGinnis Inc Project Licensing Environmental Monitoring and Mitigation Efforts See Tethys << Return to the MHK database...

  14. Robert G Wilkins MBChB FRCA CEO, Endovalve Inc.

    Office of Environmental Management (EM)

    the largest are based in Europe or elsewhere. US manufacturers frequently base their plants outside the US on economic grounds, while non-US based companies often have a...

  15. Memo from Robert Myers regarding DOE Benefit Value Desk Manual

    Energy Savers [EERE]

  16. Katie Roberts, from grad student to employee | Y-12 National...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    preventive maintenance on forklift batteries. She says her job allows her to combine lean manufacturing concepts with practical applications - that is, doing a process the...

  17. Robert C. Seamans, Jr. Appointed to Lead Nuclear Weapons Program...

    National Nuclear Security Administration (NNSA)

    C. Seamans, Jr. Appointed to Lead Nuclear Weapons Program | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile...

  18. VBA-0060- In the Matter of Robert Burd

    Broader source: Energy.gov [DOE]

    On November 16, 2001, BWXT Pantex, as successor to Mason & Hanger Corporation (M&H) (collectively referred to as “the contractor”), filed an appeal of an Initial Agency Decision (IAD)...

  19. Robert Pincus and Crispian Batstone, CIRES/Univ. Colorado and...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (the CAPT approach). * build up composites and compare modeled and observed cloud prop- erties under circumstances when the atmospheric states are similar. The similarity can...

  20. Hadron Spectroscopy from QCD Robert Edwards Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    * Can we predict hadron properties from first principles? ... arxiv:1212.0830 ORNL resources More complicated example: ... 1 st order coupled PDE's * Large, sparse, matrix solve per ...

  1. Natural Gas Compressor for Residential Use ---- Inventor Robert...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    gas compression to ultra-high pressures, which is required in many industrial and automotive processes. Gas compression, to pressures above about 100 psig, generally requires...

  2. Roberts County, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    "searchmarkers":"","locations":"text":"","title":"","link":null,"lat":35.8540979,"lon":-100.8903099,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""...

  3. EA-1902: Northern Wind Project, Roberts County, South Dakota...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    available on the project webpage, http:www.wapa.govugpEnvironmentNorthernWindFarm.htm. Public Comment Opportunities None available at this time. Documents Available for...

  4. SEMINAR SERIES Presents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Too Green?" Dr. Donald Ort Robert Emerson Professor of Plant Biology, University of Illinois at Urbana-Champaign Tuesday, November 10...

  5. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Daniel ; Shoeman, Robert L. ; Doak, R. Bruce ; Boutet, Sbastien ; et al Membrane proteins are key players in biological systems, mediating signalling events and the specific...

  6. Tobacco mosaic virus: A biological building block for micro/nano...

    Office of Scientific and Technical Information (OSTI)

    Tobacco mosaic virus: A biological building block for micronanobio systems Citation Details In-Document Search Title: Tobacco mosaic virus: A biological building block for micro...

  7. Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development ...

  8. Request for Travel Funds for Systems Radiation Biology Workshop

    SciTech Connect (OSTI)

    Barcellos-Hoff, Mary Helen

    2014-03-22

    The 3rd International Systems Radiation Biology Workshop brought together the major European, US and Japanese research programs on radiation risk as well as selected experts representing systems biological approaches to discuss how the new methodologies could be best exploited for low dose research. A significant part of the workshop was devoted to discussions organised as breakout group sessions. To facilitate discussions number of participants was limited to 60 persons. To achieve the goals of this symposium in this international conference, support from DOE is vital. Hence, this proposal requested support in the amount of $15,000 to cover the travel expenses of international experts and radiation biology scientists from the United States. This supporting mechanism was clearly identified to the selected US participants as a conference support award from the DOE (See attached PDF). The workshop was an outstanding opportunity to strengthen interactions between leading experts in the emerging areas of radiation sciences, and will also provide opportunities for younger scientists to meet with experts and discuss their results. This workshop was designed to endorse active engagement in international collaboration. A major objective of this conference was to effectively communicate research results, in order to ensure that current thinking reflects sound science of radiation biology. Further, this international event addressed the use and success of scientific initiatives in radiation biology for policymakers, standard-setters, and the general public.

  9. Solid-water detoxifying reagents for chemical and biological agents

    DOE Patents [OSTI]

    Hoffman, Dennis M.; Chiu, Ing Lap

    2006-04-18

    Formation of solid-water detoxifying reagents for chemical and biological agents. Solutions of detoxifying reagent for chemical and biological agents are coated using small quantities of hydrophobic nanoparticles by vigorous agitation or by aerosolization of the solution in the presence of the hydrophobic nanoparticles to form a solid powder. For example, when hydrophobic fumed silica particles are shaken in the presence of IN oxone solution in approximately a 95:5-weight ratio, a dry powder results. The hydrophobic silica forms a porous coating of insoluble fine particles around the solution. Since the chemical or biological agent tends to be hydrophobic on contact with the weakly encapsulated detoxifying solution, the porous coating breaks down and the detoxifying reagent is delivered directly to the chemical or biological agent for maximum concentration at the point of need. The solid-water (coated) detoxifying solutions can be blown into contaminated ventilation ducting or other difficult to reach sites for detoxification of pools of chemical or biological agent. Once the agent has been detoxified, it can be removed by flushing the area with air or other techniques.

  10. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, F.S.; Geesey, G.G.; Gillis, R.J.; Lehman, R.M.

    1997-11-11

    A method and apparatus is described for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for said microorganisms in said sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure differences in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses. 5 figs.

  11. Chemical and biological nonproliferation program. FY99 annual report

    SciTech Connect (OSTI)

    2000-03-01

    This document is the first of what will become an annual report documenting the progress made by the Chemical and Biological Nonproliferation Program (CBNP). It is intended to be a summary of the program's activities that will be of interest to both policy and technical audiences. This report and the annual CBNP Summer Review Meeting are important vehicles for communication with the broader chemical and biological defense and nonproliferation communities. The Chemical and Biological Nonproliferation Program Strategic Plan is also available and provides additional detail on the program's context and goals. The body of the report consists of an overview of the program's philosophy, goals and recent progress in the major program areas. In addition, an appendix is provided with more detailed project summaries that will be of interest to the technical community.

  12. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, Frederick S. (Idaho Falls, ID); Geesey, Gill G. (Bozeman, MT); Gillis, Richard J. (Bozeman, MT); Lehman, R. Michael (Idaho Falls, ID)

    1999-01-01

    A method and apparatus for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for said microorganisms in said sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure difference in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses.

  13. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, Frederick S. (Idaho Falls, ID); Geesey, Gill G. (Bozeman, MT); Gillis, Richard J. (Bozeman, MT); Lehman, R. Michael (Idaho Falls, ID)

    1997-01-01

    A method and apparatus for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for said microorganisms in said sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure differences in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses.

  14. Renewable Energy from Synthetic Biology (LBNL Science at the Theater)

    ScienceCinema (OSTI)

    Keasling, Jay

    2011-04-28

    Jay Keasling, co-leader of Berkeley Lab's Helios Project, is a groundbreaking researcher in the new scientific field of synthetic biology. In Helios, he directs the biology program, incorporating a range of approaches to increasing the efficacy and economy of plants and cellulose-degrading microbes to make solar-based fuels. He is a UC Berkeley professor of Chemical and Bioengineering, and founder of Amyris Biotechnologies, a company that was honored as a Technology Pioneer for 2006 by the World Economic Forum. Keasling has succeeded in using synthetic biology to develop a yeast-based production scheme for precursors of the antimalarial drug artemisinin in work funded by the Bill & Melinda Gates Foundation.

  15. Method and apparatus for determining nutrient stimulation of biological processes

    DOE Patents [OSTI]

    Colwell, F.S.; Geesey, G.G.; Gillis, R.J.; Lehman, R.M.

    1999-07-13

    A method and apparatus are disclosed for determining the nutrients to stimulate microorganisms in a particular environment. A representative sample of microorganisms from a particular environment are contacted with multiple support means wherein each support means has intimately associated with the surface of the support means a different nutrient composition for microorganisms in the sample. The multiple support means is allowed to remain in contact with the microorganisms in the sample for a time period sufficient to measure difference in microorganism effects for the multiple support means. Microorganism effects for the multiple support means are then measured and compared. The invention is particularly adaptable to being conducted in situ. The additional steps of regulating nutrients added to the particular environment of microorganisms can enhance the desired results. Biological systems particularly suitable for this invention are bioremediation, biologically enhanced oil recovery, biological leaching of metals, and agricultural bioprocesses. 5 figs.

  16. Formulations for neutralization of chemical and biological toxants

    DOE Patents [OSTI]

    Tadros, Maher E.; Tucker, Mark D.

    2003-05-20

    A formulation and method of making that neutralizes the adverse health effects of both chemical and biological compounds, especially chemical warfare (CW) and biological warfare (BW) agents. The formulation of the present invention non-toxic and non-corrosive and can be delivered by a variety of means and in different phases. The formulation provides solubilizing compounds that serve to effectively render the chemical and biological compounds, particularly CW and BW compounds, susceptible to attack and at least one reactive compound that serves to attack (and detoxify or kill) the compound. The at least one reactive compound can be an oxidizing compound, a nucleophilic compound or a mixture of both. The formulation can kill up to 99.99999% of bacterial spores within one hour of exposure.

  17. Enhanced formulations for neutralization of chemical, biological and industrial toxants

    DOE Patents [OSTI]

    Tucker, Mark D. (Albuqueque, NM) [Albuqueque, NM

    2008-06-24

    An enhanced formulation and method of making that neutralizes the adverse health effects of both chemical and biological compounds, especially chemical warfare (CW) and biological warfare (BW) agents, and toxic industrial chemicals. The enhanced formulation according to the present invention is non-toxic and non-corrosive and can be delivered by a variety of means and in different phases. The formulation provides solubilizing compounds that serve to effectively render the chemical and biological compounds, particularly CW and BW compounds, susceptible to attack, and at least one reactive compound that serves to attack (and detoxify or kill) the compound. The formulation includes at least one solubilizing agent, a reactive compound, a bleaching activator and water.

  18. Semiconductor Quantum Rods as Single Molecule FluorescentBiological Labels

    SciTech Connect (OSTI)

    Fu, Aihua; Gu, Weiwei; Boussert, Benjamine; Koski, Kristie; Gerion, Daniele; Manna, Liberato; Le Gros, Mark; Larabell, Carolyn; Alivisatos, A. Paul

    2006-05-29

    In recent years, semiconductor quantum dots have beenapplied with great advantage in a wide range of biological imagingapplications. The continuing developments in the synthesis of nanoscalematerials and specifically in the area of colloidal semiconductornanocrystals have created an opportunity to generate a next generation ofbiological labels with complementary or in some cases enhanced propertiescompared to colloidal quantum dots. In this paper, we report thedevelopment of rod shaped semiconductor nanocrystals (quantum rods) asnew fluorescent biological labels. We have engineered biocompatiblequantum rods by surface silanization and have applied them fornon-specific cell tracking as well as specific cellular targeting. Theproperties of quantum rods as demonstrated here are enhanced sensitivityand greater resistance for degradation as compared to quantum dots.Quantum rods have many potential applications as biological labels insituations where their properties offer advantages over quantumdots.

  19. Invited Review Article: Advanced light microscopy for biological space research

    SciTech Connect (OSTI)

    De Vos, Winnok H.; Beghuin, Didier; Schwarz, Christian J.; Jones, David B.; Loon, Jack J. W. A. van

    2014-10-15

    As commercial space flights have become feasible and long-term extraterrestrial missions are planned, it is imperative that the impact of space travel and the space environment on human physiology be thoroughly characterized. Scrutinizing the effects of potentially detrimental factors such as ionizing radiation and microgravity at the cellular and tissue level demands adequate visualization technology. Advanced light microscopy (ALM) is the leading tool for non-destructive structural and functional investigation of static as well as dynamic biological systems. In recent years, technological developments and advances in photochemistry and genetic engineering have boosted all aspects of resolution, readout and throughput, rendering ALM ideally suited for biological space research. While various microscopy-based studies have addressed cellular response to space-related environmental stressors, biological endpoints have typically been determined only after the mission, leaving an experimental gap that is prone to bias results. An on-board, real-time microscopical monitoring device can bridge this gap. Breadboards and even fully operational microscope setups have been conceived, but they need to be rendered more compact and versatile. Most importantly, they must allow addressing the impact of gravity, or the lack thereof, on physiologically relevant biological systems in space and in ground-based simulations. In order to delineate the essential functionalities for such a system, we have reviewed the pending questions in space science, the relevant biological model systems, and the state-of-the art in ALM. Based on a rigorous trade-off, in which we recognize the relevance of multi-cellular systems and the cellular microenvironment, we propose a compact, but flexible concept for space-related cell biological research that is based on light sheet microscopy.

  20. Detection and differentiation of coxiella burnetii in biological fluids

    DOE Patents [OSTI]

    Frazier, Marvin E. (Richland, WA); Mallavia, Louis P. (Moscow, ID); Samuel, James E. (Pullman, WA); Baca, Oswald G. (Albuquerque, NM)

    1990-01-01

    Methods for detecting the presence of Coxiella burenetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

  1. Detection and differentiation of coxiella burnetii in biological fluids

    DOE Patents [OSTI]

    Frazier, Marvin E. (Richland, WA); Mallavia, Louis P. (Moscow, ID); Samuel, James E. (Derwood, MD); Baca, Oswald G. (Albuquerque, NM)

    1993-01-01

    Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA with a DNA probe containing DNA sequences that specifically hybridize with C. burnetii DNA of strains associated with the capacity to cause acute or chronic disease.

  2. Detection and differentiation of coxiella burnetii in biological fluids

    DOE Patents [OSTI]

    Frazier, Marvin E. (Richland, WA); Mallavia, Louis P. (Moscow, ID); Baca, Oswald G. (Albuquerque, NM); Samuel, James E. (Pullman, WA)

    1989-01-01

    Methods for detecting the presence of Coxiella burnetii in biological samples, as well as a method for differentiating strains of C. burnetii that are capable of causing acute disease from those strains capable of causing chronic disease are disclosed. The methods generally comprise treating cells contained within the biological sample to expose cellular DNA, and hybridizing the cellular DNA (specifically rickettsial DNA) with a C. burnetii-specific labeled DNA probe. Radioisotope and biotin labels are preferred, allowing detection through autoradiography and colorimetric assays, respectively.

  3. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Biological Imaging by Soft X-Ray Diffraction Microscopy Print Wednesday, 30 November 2005 00:00 Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes

  4. Concentrated formulations and methods for neutralizing chemical and biological toxants

    DOE Patents [OSTI]

    Tucker, Mark D.; Betty, Rita G.; Tadros, Maher E.

    2004-04-20

    A formulation and method of making and using that neutralizes the adverse health effects of both chemical and biological toxants, especially chemical warfare (CW) and biological warfare (BW) agents. The aqueous formulation is non-toxic and non-corrosive and can be delivered as a long-lasting foam, spray, or fog. The formulation includes solubilizing compounds that serve to effectively render the CW or BW toxant susceptible to attack, so that a nucleophillic agent can attack the compound via a hydrolysis or oxidation reaction. The formulation can kill up to 99.99999% of bacterial spores within one hour of exposure.

  5. Biological and Environmental Research Advisory Committee (BERAC) meeting |

    Office of Science (SC) Website

    U.S. DOE Office of Science (SC) October 28-29, 2015 Gaithersburg, Md. Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings BERAC Minutes BERAC Minutes Archive Members Charges/Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Meetings October 28-29, 2015 Gaithersburg, Md. Print Text Size: A A A FeedbackShare Page Agenda .pdf file (61KB) Presentations: Sharlene Weatherwax .pdf file (768KB) Office of Biological and

  6. COLLOQUIUM: Plasma Mediated Effects on Biological Cells | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab October 8, 2014, 4:00pm to 5:30pm Colloquia MBG Auditorium COLLOQUIUM: Plasma Mediated Effects on Biological Cells Professor Mounir Laroussi Old Dominion University Low temperature plasma (LTP) in air-containing gas mixtures produce reactive oxygen species (ROS) such as O, O2-, and OH and reactive nitrogen species (RNS) such as NO and NO2 which exhibit strong oxidative properties and/or trigger signaling pathways in biological cells. For example oxidation of the lipids and

  7. Biological Monitoring at Amchitka Appears to Show Impacts from Fukushima

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Dai-ichi Incident | Department of Energy Biological Monitoring at Amchitka Appears to Show Impacts from Fukushima Dai-ichi Incident Biological Monitoring at Amchitka Appears to Show Impacts from Fukushima Dai-ichi Incident April 12, 2013 - 3:09pm Addthis An LM scientist points to star reindeer lichen on Adak Island, Alaska. An LM scientist points to star reindeer lichen on Adak Island, Alaska. What does this project do? Goal 1. Protect human health and the environment The U.S. Department of

  8. Computational Proteomics: High-throughput Analysis for Systems Biology

    SciTech Connect (OSTI)

    Cannon, William R.; Webb-Robertson, Bobbie-Jo M.

    2007-01-03

    High-throughput (HTP) proteomics is a rapidly developing field that offers the global profiling of proteins from a biological system. The HTP technological advances are fueling a revolution in biology, enabling analyses at the scales of entire systems (e.g., whole cells, tumors, or environmental communities). However, simply identifying the proteins in a cell is insufficient for understanding the underlying complexity and operating mechanisms of the overall system. Systems level investigations are relying more and more on computational analyses, especially in the field of proteomics generating large-scale global data.

  9. Biological Systems Science Division (BSSD) | U.S. DOE Office of Science

    Office of Science (SC) Website

    (SC) Biological Systems Science Division (BSSD) Biological and Environmental Research (BER) BER Home About Research Biological Systems Science Division (BSSD) Genomic Science DOE Bioenergy Research Centers Bioimaging Technology DOE Joint Genome Institute Structural Biology Radiochemistry & Imaging Instrumentation Radiobiology: Low Dose Radiation Research DOE Human Subjects Protection Program Climate and Environmental Sciences Division (CESD) Research Abstracts Searchable Archive of BER

  10. Biological optimization systems for enhancing photosynthetic efficiency and methods of use

    DOE Patents [OSTI]

    Hunt, Ryan W; Chinnasamy, Senthil; Das, Keshav C; Rolim de Mattos, Erico

    2014-02-25

    The present disclosure relates to biological optimization systems for enhancing photosynthetic efficiency and methods of use.

  11. Thin film composition with biological substance and method of making

    SciTech Connect (OSTI)

    Campbell, A.A.; Song, L.

    1999-09-28

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphous structures, organic crystalline structures, and organic amorphous structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobial, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflammatory, steroid, nonsteroid anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor consisting of the compositions listed above.

  12. PG&E Reconductoring Project Biological Assessment (Revised)

    Broader source: Energy.gov [DOE]

    This biological assessment (BA) is to provide the information necessary for formal consultation between the Department of Energy (DOE) and U.S. Fish and Wildlife Service (USFWS) regarding the effects of the Proposed Action as required pursuant to Section 7(a)(2) of the Endangered Species Act (ESA).

  13. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Broader source: Energy.gov [DOE]

    This technology pathway case investigates the biological conversion of biomass-derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot-scale demonstrations at NREL. Technical barriers and key research needs have been identified that should be pursued for the pathway to become competitive with petroleum-derived gasoline-, diesel-, and jet-range hydrocarbon blendstocks.

  14. Computational approaches to discovering semantics in molecular biology

    SciTech Connect (OSTI)

    Lipton, R.J. ); Marr, T.G. . Theoretical Div.); Welsh, J.D. . Dept. of Biology)

    1989-07-01

    One of the central questions of molecular biology is the discovery of the semantics of DNA. This discovery relies in a critical way on a variety of expensive computations. In order to solve these computations, both parallel computers and special-purpose hardware play a major role.

  15. Biologically Enhanced Carbon Sequestration: Research Needs and Opportunities

    SciTech Connect (OSTI)

    Oldenburg, Curtis; Oldenburg, Curtis M.; Torn, Margaret S.

    2008-03-21

    Fossil fuel combustion, deforestation, and biomass burning are the dominant contributors to increasing atmospheric carbon dioxide (CO{sub 2}) concentrations and global warming. Many approaches to mitigating CO{sub 2} emissions are being pursued, and among the most promising are terrestrial and geologic carbon sequestration. Recent advances in ecology and microbial biology offer promising new possibilities for enhancing terrestrial and geologic carbon sequestration. A workshop was held October 29, 2007, at Lawrence Berkeley National Laboratory (LBNL) on Biologically Enhanced Carbon Sequestration (BECS). The workshop participants (approximately 30 scientists from California, Illinois, Oregon, Montana, and New Mexico) developed a prioritized list of research needed to make progress in the development of biological enhancements to improve terrestrial and geologic carbon sequestration. The workshop participants also identified a number of areas of supporting science that are critical to making progress in the fundamental research areas. The purpose of this position paper is to summarize and elaborate upon the findings of the workshop. The paper considers terrestrial and geologic carbon sequestration separately. First, we present a summary in outline form of the research roadmaps for terrestrial and geologic BECS. This outline is elaborated upon in the narrative sections that follow. The narrative sections start with the focused research priorities in each area followed by critical supporting science for biological enhancements as prioritized during the workshop. Finally, Table 1 summarizes the potential significance or 'materiality' of advances in these areas for reducing net greenhouse gas emissions.

  16. Physics in medicine and biology encyclopedia 2-volume set

    SciTech Connect (OSTI)

    McAinsh, T.F.

    1986-01-01

    The Physics in Medicine and Biology Encyclopedia provides in two volumes a review of a wide range of topics of current interest in medical physics, bioengineering and biophysics. The Encyclopedia is extensively cross-referenced, indexed and illustrated, and all articles are complete with fully verified bibliographies. A glossary gives full details of specialized terms appearing in the Encyclopedia.

  17. Behavior of REE in geological and biological systems

    SciTech Connect (OSTI)

    Laul, J.C.; Weimer, W.C.

    1981-05-01

    The REE abundances when normalized to primordial (chondritic) abundances behave as a smooth function of the REE ionic radii in both the geological and biological systems. The REE are hardly fractionated chemically through various stages of their transformation from soil-soil extract-plant-geological systems.

  18. Thin film composition with biological substance and method of making

    DOE Patents [OSTI]

    Campbell, Allison A. (Kennewick, WA); Song, Lin (Richland, WA)

    1999-01-01

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphus structures, organic crystalline structures, and organic amorphus structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobal, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflamatory, steriod, nonsteriod anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor the compositions listed above.

  19. Biology Division. Progress report, August 1, 1982-September 30, 1983

    SciTech Connect (OSTI)

    Not Available

    1984-01-01

    The Biology Division is the component of the Oak Ridge National Laboratory that investigates the potential adverse health effects of energy-related substances. The body of this report provides summaries of the aims, scope and progress of the research of groups of investigators in the Division during the period of August 1, 1982, through September 30, 1983. At the end of each summary is a list of publications covering the same period (published or accepted for publication). For convenience, the summaries are assembled under Sections in accordance with the current organizational structure of the Biology Division; each Section begins with an overview. It will be apparent, however, that currents run throughout the Division and that the various programs support and interact with each other.

  20. Towards a Semantic Lexicon for Biological Language Processing

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Verspoor, Karin

    2005-01-01

    This paper explores the use of the resources in the National Library of Medicine's Unified Medical Language System (UMLS) for the construction of a lexicon useful for processing texts in the field of molecular biology. A lexicon is constructed from overlapping terms in the UMLS SPECIALIST lexicon and the UMLS Metathesaurus to obtain both morphosyntactic and semantic information for terms, and the coverage of a domain corpus is assessed. Over 77% of tokens in the domain corpus are found in the constructed lexicon, validating the lexicon's coverage of the most frequent terms in the domain and indicating that the constructedmore » lexicon is potentially an important resource for biological text processing.« less

  1. Biological responses to engineered nanomaterials: Needs for the next decade

    SciTech Connect (OSTI)

    Murphy, Catherine J.; Vartanian, Ariane M.; Geiger, Franz M.; Hamers, Robert J.; Pedersen, Joel A.; Cui, Qiang; Haynes, Christy L.; Carlson, Erin E.; Hernandez, Rigoberto; Klaper, Rebecca D.; Orr, Galya; Rosenzweig, Ze'ev

    2015-06-09

    In this study, the interaction of nanomaterials with biomolecules, cells, and organisms is an enormously vital area of current research, with applications in nanoenabled diagnostics, imaging agents, therapeutics, and contaminant removal technologies. Yet the potential for adverse biological and environmental impacts of nanomaterial exposure is considerable and needs to be addressed to ensure sustainable development of nanomaterials. In this Outlook four research needs for the next decade are outlined: (i) measurement of the chemical nature of nanomaterials in dynamic, complex aqueous environments; (ii) real-time measurements of nanomaterial-biological interactions with chemical specificity; (iii) delineation of molecular modes of action for nanomaterial effects on living systems as functions of nanomaterial properties; and (iv) an integrated systems approach that includes computation and simulation across orders of magnitude in time and space.

  2. Flow-through biological conversion of lignocellulosic biomass

    DOE Patents [OSTI]

    Herring, Christopher D.; Liu, Chaogang; Bardsley, John

    2014-07-01

    The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

  3. Biological responses to engineered nanomaterials: Needs for the next decade

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Murphy, Catherine J.; Vartanian, Ariane M.; Geiger, Franz M.; Hamers, Robert J.; Pedersen, Joel A.; Cui, Qiang; Haynes, Christy L.; Carlson, Erin E.; Hernandez, Rigoberto; Klaper, Rebecca D.; et al

    2015-06-09

    In this study, the interaction of nanomaterials with biomolecules, cells, and organisms is an enormously vital area of current research, with applications in nanoenabled diagnostics, imaging agents, therapeutics, and contaminant removal technologies. Yet the potential for adverse biological and environmental impacts of nanomaterial exposure is considerable and needs to be addressed to ensure sustainable development of nanomaterials. In this Outlook four research needs for the next decade are outlined: (i) measurement of the chemical nature of nanomaterials in dynamic, complex aqueous environments; (ii) real-time measurements of nanomaterial-biological interactions with chemical specificity; (iii) delineation of molecular modes of action for nanomaterialmore » effects on living systems as functions of nanomaterial properties; and (iv) an integrated systems approach that includes computation and simulation across orders of magnitude in time and space.« less

  4. Systems Biology in Prokaryote - Eukaryote Symbiosis | Stanford Synchrotron

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Radiation Lightsource Systems Biology in Prokaryote - Eukaryote Symbiosis Monday, June 25, 2012 - 12:00pm SLAC, SSRL Main Conference Room, 137-322 Allen M. Orville, Brookhaven National Laboratory Frontier challenges for macromolecular crystallography (MX) now include determining structures of trapped reactive intermediates, large macromolecules and viruses, membrane proteins, protein-protein complexes, and protein-nucleic acid complexes. Although structure and function are intimately linked,

  5. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  6. American Society of Agricultural and Biological Engineers Annual International Meeting

    Broader source: Energy.gov [DOE]

    The 2015 American Society of Agricultural and Biological Engineers Annual International Meeting will be held in New Orleans, Louisiana on July 26–29, 2015, and will examine industry trends and innovations, with a focus on the focus on the economic, political and social factors influencing the industry. Bioenergy Technologies Office Director Jonathan Male, Program Manager Alison Goss Eng, and Technology Managers Sam Tagore, Mark Elless, and Steve Thomas will be in attendance.

  7. Finding Balance Between Biological Groundwater Treatment and Treated Injection Water

    SciTech Connect (OSTI)

    Carlson, Mark A.; Nielsen, Kellin R.; Byrnes, Mark E.; Simmons, Sally A.; Morse, John J.; Geiger, James B.; Watkins, Louis E.; McFee, Phillip M.; Martins, K.

    2015-01-14

    At the U.S. Department of Energys Hanford Site, CH2M HILL Plateau Remediation Company operates the 200 West Pump and Treat which was engineered to treat radiological and chemical contaminants in groundwater as a result of the sites former plutonium production years. Fluidized bed bioreactors (FBRs) are used to remove nitrate, metals, and volatile organic compounds. Increasing nitrate concentrations in the treatment plant effluent and the presence of a slimy biomass (a typical microorganism response to stress) in the FBRs triggered an investigation of nutrient levels in the system. Little, if any, micronutrient feed was coming into the bioreactors. Additionally, carbon substrate (used to promote biological growth) was passing through to the injection wells, causing biological fouling of the wells and reduced specific injectivity. Adjustments to the micronutrient feed improved microorganism health, but the micronutrients were being overfed (particularly manganese) plugging the injection wells further. Injection well rehabilitation to restore specific injectivity required repeated treatments to remove the biological fouling and precipitated metal oxides. A combination of sulfamic and citric acids worked well to dissolve metal oxides and sodium hypochlorite effectively removed the biological growth. Intensive surging and development techniques successfully removed clogging material from the injection wells. Ultimately, the investigation and nutrient adjustments took months to restore proper balance to the microbial system and over a year to stabilize injection well capacities. Carefully tracking and managing the FBRs and well performance monitoring are critical to balancing the needs of the treatment system while reducing fouling mechanisms in the injection wells.

  8. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  9. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  10. The Biological Implications of the PP2A Crystal Structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Biological Implications of the PP2A Crystal Structure Print Phosphatases, enzymes that remove a phosphate group from amino-acid substrates, can be subdivided according to their substrate specificity. Myriad evidence has demonstrated that protein phosphatase 2A (PP2A), a family of serine/threonine-specific (Ser/Thr) phosphatases, regulates many, if not most, aspects of cellular activities and is a critical tumor suppressor. A team at the University of Washington recently determined the first

  11. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Conversion of Sugars to Hydrocarbons Technology Pathway Ryan Davis, Mary Biddy, Eric Tan, and Ling Tao National Renewable Energy Laboratory Susanne Jones Pacific Northwest National Laboratory NREL is a national laboratory of the U.S. Department of Energy, Office of Energy Efficiency & Renewable Energy, operated by the Alliance for Sustainable Energy, LLC, under contract DE-AC36-08GO28308. Pacific Northwest National Laboratory is operated by Battelle for the United States

  12. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  13. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  14. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  15. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  16. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  17. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  18. Detection of biological molecules using chemical amplification and optical sensors

    DOE Patents [OSTI]

    Van Antwerp, William Peter; Mastrototaro, John Joseph

    2004-10-12

    Methods are provided for the determination of the concentration of biological levels of polyhydroxylated compounds, particularly glucose. The methods utilize an amplification system that is an analyte transducer immobilized in a polymeric matrix, where the system is implantable and biocompatible. Upon interrogation by an optical system, the amplification system produces a signal capable of detection external to the skin of the patient. Quantitation of the analyte of interest is achieved by measurement of the emitted signal.

  19. Detection of biological molecules using chemical amplification and optical sensors

    DOE Patents [OSTI]

    Van Antwerp, William Peter (Valencia, CA); Mastrototaro, John Joseph (Los Angeles, CA)

    2000-01-01

    Methods are provided for the determination of the concentration of biological levels of polyhydroxylated compounds, particularly glucose. The methods utilize an amplification system that is an analyte transducer immobilized in a polymeric matrix, where the system is implantable and biocompatible. Upon interrogation by an optical system, the amplification system produces a signal capable of detection external to the skin of the patient. Quantitation of the analyte of interest is achieved by measurement of the emitted signal.

  20. Synthetic Biology: Engineering, Evolution and Design (SEED) Conference 2014

    SciTech Connect (OSTI)

    Voigt, Christopher

    2014-07-01

    SEED2014 focused on advances in the science and technology emerging from the field of synthetic biology. We broadly define this as technologies that accelerate the process of genetic engineering. It highlighted new tool development, as well as the application of these tools to diverse problems in biotechnology, including therapeutics, industrial chemicals and fuels, natural products, and agriculture. Systems spanned from in vitro experiments and viruses, through diverse bacteria, to eukaryotes (yeast, mammalian cells, plants).

  1. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  2. Biological Imaging by Soft X-Ray Diffraction Microscopy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Imaging by Soft X-Ray Diffraction Microscopy Print Electron and x-ray microscopes are a valuable tool for both the life and materials sciences, but they are limited in their ability to image with nanometer-scale resolution in three dimensions nonperiodic objects that are several microns in size. To fill this gap, the technique of coherent x-ray diffraction imaging now under development takes advantage of the penetrating power of x rays while simultaneously removing the limitations

  3. Biology Chemistry & Material Science Laboratory 1 | Sample Preparation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratories 1 Cynthia Patty | (650) 926-3925 Biology Chemistry & Material Science Laboratory 1 Inventory The BioChemMat Lab 1 at SSRL is dedicated to researcher experiments, including x-ray absorption and emission spectroscopies, macromolecular crystallography, x-ray scattering, and x-ray imaging. The labs are maintained for final-stage sample preparation and other relatively straight-forward laboratory manipulations. These include buffer preparations, solid sample grinding, solution

  4. Biology Chemistry & Material Science Laboratory 2 | Sample Preparation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratories 2 Cynthia Patty | (650) 926-3925 Biology Chemistry & Material Science Laboratory 2 Inventory The BioChemMat Lab 2 (BCM 2) at SSRL is dedicated to researcher experiments, including x-ray absorption and emission spectroscopies, macromolecular crystallography, x-ray scattering, and x-ray imaging. The labs are maintained for final-stage sample preparation and other relatively straight-forward laboratory manipulations. These include buffer preparations, solid sample grinding,

  5. CRC handbook of biological effects of electromagnetic fields

    SciTech Connect (OSTI)

    Polk, C.; Postow, E.

    1986-01-01

    This book presents the current knowledge about the effects of electromagnetic fields on living matter. The three-part format covers dielectric permittivity and electrical conductivity of biological materials; effects of direct current and low frequency fields; and effects of radio frequency (including microwave) fields. The parts are designed to be consulted independently or in sequence, depending upon the needs of the reader. Useful appendixes on measurement units and safety standards are also included.

  6. In Situ Biological Uranium Remediation within a Highly Contaminated Aquifer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In Situ Biological Uranium Remediation within a Highly Contaminated Aquifer Matthew Ginder-Vogel1, Wei-Min Wu1, Jack Carley2, Phillip Jardine2, Scott Fendorf1 and Craig Criddle1 1Stanford University, Stanford, CA 2Oak Ridge National Laboratory, Oak Ridge, TN Microbial Respiration Figure 1. Uranium(VI) reduction is driven by microbial respiration resulting in the precipitation of uraninite. Uranium contamination of ground and surface waters has been detected at numerous sites throughout the

  7. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Conversion of Sugars to Hydrocarbons Technology Pathway Ryan Davis, Mary Biddy, Eric Tan, and Ling Tao National Renewable Energy Laboratory Susanne Jones Pacific Northwest National Laboratory NREL is a national laboratory of the U.S. Department of Energy, Office of Energy Efficiency & Renewable Energy, operated by the Alliance for Sustainable Energy, LLC, under contract DE-AC36-08GO28308. Pacific Northwest National Laboratory is operated by Battelle for the United States

  8. PROGRESS REPORT ON THE BIOLOGICAL MONITORING PROGRAM FOR THE

    Office of Legacy Management (LM)

    PROGRESS REPORT ON THE BIOLOGICAL MONITORING PROGRAM FOR THE MONTICELLO, UTAH, MILL SITE: AUGUST 1996 SAMPLING PERIOD J. G. Smith M. J. Peterson M. G. Ryon G. R. Southworth Date: March 3, 1997 Prepared for G. A. Pierce Health and Safety Research Division Environmental Technology Section OakRidge National Laboratory Grand Junction, Colorado Prepared by Environmental Sciences Division Oak Ridge National Laboratory Oak Ridge, Tennessee 37831 Managed by LOCKHEED MARTIN ENERGY RESEARCH CORP. for the

  9. Mathematics and biology: The interface, challenges and opportunities

    SciTech Connect (OSTI)

    Levin, S.A. )

    1992-06-01

    The interface between mathematics and biology has long been a rich area of research, with mutual benefit to each supporting discipline. Traditional areas of investigation, such as population genetics, ecology, neurobiology, and 3-D reconstructions, have flourished, despite a rather meager environment for the funding of such work. In the past twenty years, the kind and scope of such interactions between mathematicians and biologists have changed dramatically, reaching out to encompass areas of both biology and mathematics that previously had not benefited. At the same time, with the closer integration of theory and experiment, and the increased reliance on high-speed computation, the costs of such research grew, though not the opportunities for funding. The perception became reinforced, both within the research community and at funding agencies, that although these interactions were expanding, they were not doing so at the rate necessary to meet the opportunities and needs. A workshop was held in Washington, DC, between April 28 and May 3, 1990 which drew together a broadly based group of researchers to synthesize conclusions from a group of working papers and extended discussions. The result is the report presented here, which we hope will provide a guide and stimulus to research in mathematical and computational biology for at least the next decade. The report identifies a number of grand challenges, representing a broad consensus among the participants.

  10. Polarized Neutron in Structural Biology Present and Future Outlook

    SciTech Connect (OSTI)

    Zhao, Jinkui [ORNL; Robertson, Lee [ORNL; Herwig, Kenneth W [ORNL; Crabb, Don [University of Virginia

    2013-01-01

    Hydrogen has a strong polarization-dependent neutron scattering cross section. This property has been exploited in the study of soft matters, especially biological macromolecules. When a polarized neutron beam is scattered off a polarized hydrogenous sample, the otherwise large hydrogen incoherent cross section is drastically reduced while the coherent signal is significantly increased. Past experiments have demonstrated the potentials and benefits of polarized neutron scattering from soft materials. The main technical challenge of polarized neutron scattering from biological matters lies at sample polarization. Dynamic nuclear polarization is a proven yet rather sophisticated technique. Its complexity is one of the main reasons for the technique's slow adoption. The future of polarized neutron scattering in biology may rest largely in neutron protein crystallography. Polarization of protein crystals is much easier to accomplish, since protein crystals are typically rather small (<<1 mm) and only require small and easy- to-operate polarization apparatuses. In addition, the high resolution nature of neutron protein crystallography means that we will be able to study individual atoms using the polarized neutron scattering technique.

  11. Experimental Data from the Proteomics Research Center for Integrative Biology

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Smith, Richard D.

    The possible roles and importance of proteomics are rapidly growing across essentially all areas of biological research. The precise and comprehensive measurement of levels of expressed proteins and their modified forms can provide new insights into the molecular nature of cell-signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to understanding human health and the progression of various disease states. The ability to characterize protein complexes complements this capability, allowing hypotheses to be tested and the biological system operation to be defined. The Proteomics Research Center for Integrative Biology is a national user facility established and funded by the National Institute of General Medical Sciences component of the National Institutes of Health. This Center has been established to serve the biomedical research community by developing and integrating new proteomic technologies for collaborative and service studies, disseminating the new technologies, and training scientists in their use. The Center is housed in DOEs William R. Wiley Environmental Molecular Sciences Laboratory (EMSL) at the Pacific Northwest National Laboratory.

  12. Experimental Data from the Proteomics Research Center for Integrative Biology

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Smith, Richard D.

    The possible roles and importance of proteomics are rapidly growing across essentially all areas of biological research. The precise and comprehensive measurement of levels of expressed proteins and their modified forms can provide new insights into the molecular nature of cell-signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to understanding human health and the progression of various disease states. The ability to characterize protein complexes complements this capability, allowing hypotheses to be tested and the biological system operation to be defined. The Proteomics Research Center for Integrative Biology is a national user facility established and funded by the National Institute of General Medical Sciences component of the National Institutes of Health. This Center has been established to serve the biomedical research community by developing and integrating new proteomic technologies for collaborative and service studies, disseminating the new technologies, and training scientists in their use. The Center is housed in DOE’s William R. Wiley Environmental Molecular Sciences Laboratory (EMSL) at the Pacific Northwest National Laboratory.

  13. Method of and apparatus for determining the similarity of a biological analyte from a model constructed from known biological fluids

    DOE Patents [OSTI]

    Robinson, Mark R.; Ward, Kenneth J.; Eaton, Robert P.; Haaland, David M.

    1990-01-01

    The characteristics of a biological fluid sample having an analyte are determined from a model constructed from plural known biological fluid samples. The model is a function of the concentration of materials in the known fluid samples as a function of absorption of wideband infrared energy. The wideband infrared energy is coupled to the analyte containing sample so there is differential absorption of the infrared energy as a function of the wavelength of the wideband infrared energy incident on the analyte containing sample. The differential absorption causes intensity variations of the infrared energy incident on the analyte containing sample as a function of sample wavelength of the energy, and concentration of the unknown analyte is determined from the thus-derived intensity variations of the infrared energy as a function of wavelength from the model absorption versus wavelength function.

  14. Grays River Watershed and Biological Assessment Final Report 2006.

    SciTech Connect (OSTI)

    May, Christopher W.; McGrath, Kathleen E.; Geist, David R.; Abbe, Timothy; Barton, Chase

    2008-02-04

    The Grays River Watershed and Biological Assessment was funded to address degradation and loss of spawning habitat for chum salmon (Onchorhynchus keta) and fall Chinook salmon (Onchoryhnchus tshawytscha). In 1999, the National Marine Fisheries Service listed lower Columbia River chum salmon as a threatened Evolutionarily Significant Unit (ESU) under the Endangered Species Act of 1973 (ESA). The Grays River watershed is one of two remaining significant chum salmon spawning locations in this ESU. Runs of Grays River chum and Chinook salmon have declined significantly during the past century, largely because of damage to spawning habitat associated with timber harvest and agriculture in the watershed. In addition, approximately 20-25% of the then-remaining chum salmon spawning habitat was lost during a 1999 channel avulsion that destroyed an important artificial spawning channel operated by the Washington Department of Fish and Wildlife (WDFW). Although the lack of stable, high-quality spawning habitat is considered the primary physical limitation on Grays River chum salmon production today, few data are available to guide watershed management and channel restoration activities. The objectives of the Grays River Watershed and Biological Assessment project were to (1) perform a comprehensive watershed and biological analysis, including hydrologic, geomorphic, and ecological assessments; (2) develop a prioritized list of actions that protect and restore critical chum and Chinook salmon spawning habitat in the Grays River based on comprehensive geomorphic, hydrologic, and stream channel assessments; and (3) gain a better understanding of chum and Chinook salmon habitat requirements and survival within the lower Columbia River and the Grays River. The watershed-based approach to river ecosystem restoration relies on a conceptual framework that describes general relationships between natural landscape characteristics, watershed-scale habitat-forming processes, aquatic habitat conditions, and biological integrity. In addition, human land-use impacts are factored into the conceptual model because they can alter habitat quality and can disrupt natural habitat-forming processes. In this model (Figure S.1), aquatic habitat--both instream and riparian--is viewed as the link between watershed conditions and biologic responses. Based on this conceptual model, assessment of habitat loss and the resultant declines in salmonid populations can be conducted by relating current and historical (e.g., natural) habitat conditions to salmonid utilization, diversity, and abundance. In addition, assessing disrupted ecosystem functions and processes within the watershed can aid in identifying the causes of habitat change and the associated decline in biological integrity. In this same way, restoration, enhancement, and conservation projects can be identified and prioritized. A watershed assessment is primarily a landscape-scale evaluation of current watershed conditions and the associated hydrogeomorphic riverine processes. The watershed assessment conducted for this project focused on watershed processes that form and maintain salmonid habitat. Landscape metrics describing the level of human alteration of natural ecosystem attributes were used as indicators of water quality, hydrology, channel geomorphology, instream habitat, and biotic integrity. Ecological (watershed) processes are related to and can be predicted based on specific aspects of spatial pattern. This study evaluated the hydrologic regime, sediment delivery regime, and riparian condition of the sub-watersheds that comprise the upper Grays River watershed relative to their natural range of conditions. Analyses relied primarily on available geographic information system (GIS) data describing landscape characteristics such as climate, vegetation type and maturity, geology and soils, topography, land use, and road density. In addition to watershed-scale landscape characteristics, the study area was also evaluated on the riparian scale, with appropriate landscape variables analyzed within

  15. Novel nuclear magnetic resonance techniques for studying biological molecules

    SciTech Connect (OSTI)

    Laws, David D.

    2000-06-01

    Over the fifty-five year history of Nuclear Magnetic Resonance (NMR), considerable progress has been made in the development of techniques for studying the structure, function, and dynamics of biological molecules. The majority of this research has involved the development of multi-dimensional NMR experiments for studying molecules in solution, although in recent years a number of groups have begun to explore NMR methods for studying biological systems in the solid-state. Despite this new effort, a need still exists for the development of techniques that improve sensitivity, maximize information, and take advantage of all the NMR interactions available in biological molecules. In this dissertation, a variety of novel NMR techniques for studying biomolecules are discussed. A method for determining backbone ({phi}/{psi}) dihedral angles by comparing experimentally determined {sup 13}C{sub a}, chemical-shift anisotropies with theoretical calculations is presented, along with a brief description of the theory behind chemical-shift computation in proteins and peptides. The utility of the Spin-Polarization Induced Nuclear Overhauser Effect (SPINOE) to selectively enhance NMR signals in solution is examined in a variety of systems, as are methods for extracting structural information from cross-relaxation rates that can be measured in SPINOE experiments. Techniques for the production of supercritical and liquid laser-polarized xenon are discussed, as well as the prospects for using optically pumped xenon as a polarizing solvent. In addition, a detailed study of the structure of PrP 89-143 is presented. PrP 89-143 is a 54 residue fragment of the prion proteins which, upon mutation and aggregation, can induce prion diseases in transgenic mice. Whereas the structure of the wild-type PrP 89-143 is a generally unstructured mixture of {alpha}-helical and {beta}-sheet conformers in the solid state, the aggregates formed from the PrP 89-143 mutants appear to be mostly {beta}-sheet.

  16. 2010 Tetrapyrroles, Chemistry & Biology of Gordon Research Conference

    SciTech Connect (OSTI)

    Angela Wilks

    2010-07-30

    The objective of the Chemistry & Biology of Tetrapyrroles Gordon Conference is to bring together researchers from diverse disciplines that otherwise would not interact. By bringing biologists, chemists, engineers and clinicians with a common interest in tetrapyrroles the conference provides a forum for cross-disciplinary ideas and collaboration. The perspective provided by biologists, chemists, and clinicians working in fields such as newly discovered defects in human porphyrin metabolism, the myriad of strategies for light harvesting in photosynthetic organisms, novel tetrapyrroles that serve as auxiliary chromophores or enzyme cofactors, synthetic strategies in the design of novel tetrapyrrole scaffolds, and tetrapyrrole based cell signaling and regulatory systems, makes this conference unique in the field. Over the years the growing evidence for the role of tetrapyrroles and their reactive intermediates in cell signaling and regulation has been of increasing importance at this conference. The 2010 conference on Chemistry & Biology of Tetrapyrroles will focus on many of these new frontiers as outlined in the preliminary program listed. Speakers will emphasize unpublished results and new findings in the field. The oral sessions will be followed by the highly interactive afternoon poster sessions. The poster sessions provide all conferees with the opportunity to present their latest research and to exchange ideas in a more informal setting. As in the past, this opportunity will continue during the nightly social gathering that takes place in the poster hall following the evening lectures. All conferees are encouraged to submit and present posters. At the conference the best poster in the areas of biology, chemistry and medicine will be selected by a panel of previous conference chairs.

  17. PROTECT: Enhanced Technology to Protect Against Chemical and Biological

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Terrorism - Energy Innovation Portal Energy Analysis Energy Analysis Find More Like This Return to Search PROTECT: Enhanced Technology to Protect Against Chemical and Biological Terrorism Argonne National Laboratory Contact ANL About This Technology <p class="Default" style="margin: 4pt 0in 2pt; line-height: 8.05pt;"> <i><span style="color: windowtext; font-family: &quot;Franklin Gothic Medium&quot;,&quot;sans-serif&quot;; font-size:

  18. Biological and chemical technologies research. FY 1995 annual summary report

    SciTech Connect (OSTI)

    1996-03-01

    The annual summary report presents the fiscal year (FY) 1995 research activities and accomplishments for the United States Department of Energy (DOE) Biological and Chemical Technologies Research (BCTR) Program. This BCTR program resides within the Office of Industrial Technologies (OIT) of the Office of Energy Efficiency and Renewable Energy (EE). The annual summary report for 1995 (ASR 95) contains the following: program description (including BCTR program mission statement, historical background, relevance, goals and objectives); program structure and organization, selected technical and programmatic highlights for 1995; detailed descriptions of individual projects; a listing of program output, including a bibliography of published work; patents; and awards arising from work supported by the BCTR.

  19. Detection of biological molecules using chemical amplification and optical sensors

    DOE Patents [OSTI]

    Van Antwerp, William Peter (Valencia, CA); Mastrototaro, John Joseph (Los Angeles, CA)

    2001-01-01

    Methods are provided for the determination of the concentration of biological levels of polyhydroxylated compounds, particularly glucose. The methods utilize an amplification system that is an analyte transducer immobilized in a polymeric matrix, where the system is implantable and biocompatible. Upon interrogation by an optical system, the amplification system produces a signal capable of detection external to the skin of the patient. Quantitation of the analyte of interest is achieved by measurement of the emitted signal. Specifically, the analyte transducer immobilized in a polymeric matrix can be a boronic acid moiety.

  20. Dual fiber microprobe for mapping elemental distributions in biological cells

    DOE Patents [OSTI]

    Martin, Rodger C [Powell, TN; Martin, Madhavi Z [Powell, TN

    2007-07-31

    Laser-induced breakdown spectroscopy (LIBS) is applied on a microscale for in situ elemental analysis and spatial mapping in biological cells. A high power laser beam is focused onto a cell surface using a dual branching optical fiber probe for optical excitation of the cell constituents. Dual spectrometers and ICCD detectors capture the emission spectra from the excited cell(s). Repeated probing or repositioning of the laser beam with respect to the cell can provide 2-D or 3-D mapping of the cell.

  1. Proliferation profile assessment of emerging biological weapons threats. Research paper

    SciTech Connect (OSTI)

    Archuleta, M.G.; Bland, M.S.; Duann, T.P.; Tucker, A.B.

    1996-04-01

    This project develops a first generation biological warfare (BW) program system model for use in the DOD`s counterproliferation workstation, identifies key issues the U.S. military must address to assure its forces are prepared to fight in a BW environment, and develops a concise BW primer for use by any DOD activity requiring such information. The greatest lesson to be learned from this study is that only through the collective analysis of all the sub-systems of a suspected BW program will conclusive evidence of the program be found. Still, with current counterproliferation capabilities the U.S. may only be able to slow, not stop a motivated proliferant.

  2. Method and apparatus to image biological interactions in plants

    DOE Patents [OSTI]

    Weisenberger, Andrew; Bonito, Gregory M.; Reid, Chantal D.; Smith, Mark Frederick

    2015-12-22

    A method to dynamically image the actual translocation of molecular compounds of interest in a plant root, root system, and rhizosphere without disturbing the root or the soil. The technique makes use of radioactive isotopes as tracers to label molecules of interest and to image their distribution in the plant and/or soil. The method allows for the study and imaging of various biological and biochemical interactions in the rhizosphere of a plant, including, but not limited to, mycorrhizal associations in such regions.

  3. Bevatron/Bevalac user's handbook: biology and medicine. Revision

    SciTech Connect (OSTI)

    Not Available

    1985-04-01

    The Bevalac Biomedical Facility develops a source of near-relativistic heavy ions for applications to radiation biology, radiation therapy and diagnostic radiology. Pulsed beams of high LET heavy ions with variable pulse width, frequency, intensity and energy are produced and delivered to the Biomedical Facility by the Bevatron/Bevalac accelerator complex. Dosimetry equipment under computer control provides accurate determinations of absorbed doses in all regions of the Bragg curve. Depth-dose modifying devices and precise specimen positioning equipment are available. Animal housing and tissue culture facilities are convenient to the experimenter. This handbook is designed to provide the user with the relevant information for planning, proposing and executing an experiment.

  4. Center for Lignocellulose Structure and Function - Contact Us

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contact Us Daniel Cosgrove, Director Biology Department, Pennsylvania State University Phone: 814-863-3892 Email: dcosgrove@psu.edu Candace Haigler, Associate Director Department of Crop Sciences, North Carolina State University Phone: 540-231-4601 Email: Candace_Haigler@ncsu.edu Laura Ullrich (Gilliland), Manager Office Location: South Frear 304A Mailing address: Mueller 208, Biology The Pennsylvania State University University Park, PA 16802 email: LUG11@psu.edu Telephone: (814) 867-4132 For

  5. A national facility for biological cryo-electron microscopy

    SciTech Connect (OSTI)

    Saibil, Helen R.; Grnewald, Kay; Stuart, David I.

    2015-01-01

    This review provides a brief update on the use of cryo-electron microscopy for integrated structural biology, along with an overview of the plans for the UK national facility for electron microscopy being built at the Diamond synchrotron. Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an understanding of the molecular machinery of cells, disease processes and the actions of pathogenic organisms from atomic detail through to the cellular context. However, cutting-edge research in this field requires very substantial resources for equipment, infrastructure and expertise. Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. State-of-the-art equipment operated with expert support will be provided, optimized for both atomic-level single-particle analysis of purified macromolecules and complexes and for tomography of cell sections. The access to and organization of the facility will be modelled on the highly successful macromolecular crystallography (MX) synchrotron beamlines, and will be embedded at the Diamond Light Source, facilitating the development of user-friendly workflows providing near-real-time experimental feedback.

  6. Deposition of biological aerosols on HVAC heat exchangers

    SciTech Connect (OSTI)

    Siegel, Jeffrey; Walker, Ian

    2001-09-01

    Many biologically active materials are transported as bioaerosols 1-10 {micro}m in diameter. These particles can deposit on cooling and heating coils and lead to serious indoor air quality problems. This paper investigates several of the mechanisms that lead to aerosol deposition on fin and tube heat exchangers. A model has been developed that incorporates the effects of several deposition mechanisms, including impaction, Brownian and turbulent diffusion, turbophoresis, thermophoresis, diffusiophoresis, and gravitational settling. The model is applied to a typical range of air velocities that are found in commercial and residential HVAC systems 1 - 6 m/s (200 - 1200 ft/min), particle diameters from 1 - 8 {micro}m, and fin spacings from 3.2 - 7.9 fins/cm (8 - 16 fins/inch or FPI). The results from the model are compared to results from an experimental apparatus that directly measures deposition on a 4.7 fins/cm (12 FPI) coil. The model agrees reasonably well with this measured data and suggests that cooling coils are an important sink for biological aerosols and consequently a potential source of indoor air quality problems.

  7. WHOLE CELL TOMOGRAPHY/MOLECULAR BIOLOGY/STRUCTURAL BIOLOGY: Affordable x-ray microscopy with nanoscale resolution

    SciTech Connect (OSTI)

    Evans, James E.; Blackborow, Paul; Horne, Stephen J.; Gelb, Jeff

    2013-03-01

    Biological research spans 10 orders of magnitude from angstroms to meters. While electron microscopy can reveal structural details at most of these spatial length scales, transmission electron tomography only reliably reconstructs three-dimensional (3-D) volumes of cellular material with a spatial resolution between 1-5 nm from samples less than 500 nm thick1. Most biological cells are 2-30 times thicker than this threshold, which means that a cell must be cut into consecutive slices with each slice reconstructed individually in order to approximate the contextual information of the entire cell. Fortunately, due to a larger penetration depth2, X-ray computed tomography bypasses the need to physically section a cell and enables imaging of intact cells and tissues on the micrometer or larger scale with tens to hundreds of nanometer spatial resolution. While the technique of soft x-ray microscopy has been extensively developed in synchrotron facilities, advancements in laboratory x-ray source designs now increase its accessibility by supporting commercial systems suitable for a standard laboratory. In this paper, we highlight a new commercial compact cryogenic soft x-ray microscope designed for a standard laboratory setting and explore its capabilities for mesoscopic investigations of intact prokaryotic and eukaryotic cells.

  8. BCTR: Biological and Chemical Technologies Research 1994 annual summary report

    SciTech Connect (OSTI)

    Petersen, G.

    1995-02-01

    The annual summary report presents the fiscal year (FY) 1994 research activities and accomplishments for the United States Department of Energy (DOE) Biological and Chemical Technologies Research (BCTR) Program of the Advanced Industrial Concepts Division (AICD). This AICD program resides within the Office of Industrial Technologies (OIT) of the Office of Energy Efficiency and Renewable Energy (EE). Although the OIT was reorganized in 1991 and AICD no longer exists, this document reports on efforts conducted under the former structure. The annual summary report for 1994 (ASR 94) contains the following: program description (including BCTR program mission statement, historical background, relevance, goals and objectives); program structure and organization, selected technical and programmatic highlights for 1994; detailed descriptions of individual projects; a listing of program output, including a bibliography of published work; patents, and awards arising from work supported by BCTR.

  9. Biological imaging by soft x-ray diffraction microscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shapiro, D.; Thibault, P.; Beetz, T.; Elser, V.; Howells, M.; Jacobsen, C.; Kirz, J.; Lima, E.; Miao, H.; Neiman, A. M.; et al

    2005-10-25

    We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffractionmore » microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.« less

  10. Oxidizer gels for detoxification of chemical and biological agents

    DOE Patents [OSTI]

    Hoffman, Dennis M.; McGuire, Raymond R.

    2002-01-01

    A gel composition containing oxidizing agents and thickening or gelling agents is used to detoxify chemical and biological agents by application directly to a contaminated area. The gelling agent is a colloidal material, such as silica, alumina, or alumino-silicate clays, which forms a viscous gel that does not flow when applied to tilted or contoured surfaces. Aqueous or organic solutions of oxidizing agents can be readily gelled with less than about 30% colloidal material. Gel preparation is simple and suitable for field implementation, as the gels can be prepared at the site of decontamination and applied quickly and uniformly over an area by a sprayer. After decontamination, the residue can be washed away or vacuumed up for disposal.

  11. Chemical imaging of biological materials by NanoSIMS

    SciTech Connect (OSTI)

    Weber, P K; Smith, J B; Hutcheon, I D; Shmakov, A; Rybitskaya, I; Curran, H

    2004-08-23

    The NanoSIMS 50 represents the state -of-the-art for in situ microanalysis for secondary ion mass spectrometry (SIMS), combining unprecedented spatial resolution (as good as 50 nm) with ultra-high sensitivity (MDL of 200 atoms). The NanoSIMS incorporates an array of detectors, enabling simultaneous collection of 5 elements or isotopes originating from the same sputtered volume of a sample. The primary ion beam (Cs{sup +} or O{sup -}) can be scanned across the sample to produce quantitative secondary ion images. This capability for multiple isotope imaging with high spatial resolution is unique to the NanoSIMS and provides a novel new approach to the study of the distribution of elements in biological materials. We have applied this technique extensively to mammalian cells and bacterial spores. Results from these studies and critical analytical issues such as sample preparation, instrument tuning, and data processing will be discussed.

  12. Method and apparatus for electrostatically sorting biological cells

    DOE Patents [OSTI]

    Merrill, John T. (Berkeley, CA)

    1982-01-01

    An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.

  13. Engineered plant biomass particles coated with biological agents

    DOE Patents [OSTI]

    Dooley, James H.; Lanning, David N.

    2014-06-24

    Plant biomass particles coated with a biological agent such as a bacterium or seed, characterized by a length dimension (L) aligned substantially parallel to a grain direction and defining a substantially uniform distance along the grain, a width dimension (W) normal to L and aligned cross grain, and a height dimension (H) normal to W and L. In particular, the L.times.H dimensions define a pair of substantially parallel side surfaces characterized by substantially intact longitudinally arrayed fibers, the W.times.H dimensions define a pair of substantially parallel end surfaces characterized by crosscut fibers and end checking between fibers, and the L.times.W dimensions define a pair of substantially parallel top and bottom surfaces.

  14. Biological Conversion of Sugars to Hydrocarbons Technology Pathway

    SciTech Connect (OSTI)

    Davis, Ryan; Biddy, Mary J.; Tan, Eric; Tao, Ling; Jones, Susanne B.

    2013-03-31

    In support of the Bioenergy Technologies Office, the National Renewable Energy Laboratory (NREL) and the Pacific Northwest National Laboratory (PNNL) are undertaking studies of biomass conversion technologies to identify barriers and target research toward reducing conversion costs. Process designs and preliminary economic estimates for each of these pathway cases were developed using rigorous modeling tools (Aspen Plus and Chemcad). These analyses incorporated the best information available at the time of development, including data from recent pilot and bench-scale demonstrations, collaborative industrial and academic partners, and published literature and patents. This technology pathway case investigates the biological conversion of biomass derived sugars to hydrocarbon biofuels, utilizing data from recent literature references and information consistent with recent pilot scale demonstrations at NREL. Technical barriers and key research needs have been identified that should be pursued for the pathway to become competitive with petroleum-derived gasoline, diesel and jet range hydrocarbon blendstocks.

  15. Biological Information Document, Radioactive Liquid Waste Treatment Facility

    SciTech Connect (OSTI)

    Biggs, J.

    1995-12-31

    This document is intended to act as a baseline source material for risk assessments which can be used in Environmental Assessments and Environmental Impact Statements. The current Radioactive Liquid Waste Treatment Facility (RLWTF) does not meet current General Design Criteria for Non-reactor Nuclear Facilities and could be shut down affecting several DOE programs. This Biological Information Document summarizes various biological studies that have been conducted in the vicinity of new Proposed RLWTF site and an Alternative site. The Proposed site is located on Mesita del Buey, a mess top, and the Alternative site is located in Mortandad Canyon. The Proposed Site is devoid of overstory species due to previous disturbance and is dominated by a mixture of grasses, forbs, and scattered low-growing shrubs. Vegetation immediately adjacent to the site is a pinyon-juniper woodland. The Mortandad canyon bottom overstory is dominated by ponderosa pine, willow, and rush. The south-facing slope was dominated by ponderosa pine, mountain mahogany, oak, and muhly. The north-facing slope is dominated by Douglas fir, ponderosa pine, and oak. Studies on wildlife species are limited in the vicinity of the proposed project and further studies will be necessary to accurately identify wildlife populations and to what extent they utilize the project area. Some information is provided on invertebrates, amphibians and reptiles, and small mammals. Additional species information from other nearby locations is discussed in detail. Habitat requirements exist in the project area for one federally threatened wildlife species, the peregrine falcon, and one federal candidate species, the spotted bat. However, based on surveys outside of the project area but in similar habitats, these species are not expected to occur in either the Proposed or Alternative RLWTF sites. Habitat Evaluation Procedures were used to evaluate ecological functioning in the project area.

  16. Grays River Watershed and Biological Assessment, 2006 Final Report.

    SciTech Connect (OSTI)

    May, Christopher; Geist, David

    2007-04-01

    The Grays River Watershed and Biological Assessment was funded to address degradation and loss of spawning habitat for chum salmon (Onchorhynchus keta) and fall Chinook salmon (Onchoryhnchus tshawytscha). In 1999, the National Marine Fisheries Service listed lower Columbia River chum salmon as a threatened Evolutionarily Significant Unit (ESU) under the Endangered Species Act of 1973 (ESA). The Grays River watershed is one of two remaining significant chum salmon spawning locations in this ESU. Runs of Grays River chum and Chinook salmon have declined significantly during the past century, largely because of damage to spawning habitat associated with timber harvest and agriculture in the watershed. In addition, approximately 20-25% of the then-remaining chum salmon spawning habitat was lost during a 1999 channel avulsion that destroyed an important artificial spawning channel operated by the Washington Department of Fish and Wildlife (WDFW). Although the lack of stable, high-quality spawning habitat is considered the primary physical limitation on Grays River chum salmon production today, few data are available to guide watershed management and channel restoration activities. The objectives of the Grays River Watershed and Biological Assessment project were to (1) perform a comprehensive watershed and biological analysis, including hydrologic, geomorphic, and ecological assessments; (2) develop a prioritized list of actions that protect and restore critical chum and Chinook salmon spawning habitat in the Grays River based on comprehensive geomorphic, hydrologic, and stream channel assessments; and (3) gain a better understanding of chum and Chinook salmon habitat requirements and survival within the lower Columbia River and the Grays River. The watershed-based approach to river ecosystem restoration relies on a conceptual framework that describes general relationships between natural landscape characteristics, watershed-scale habitat-forming processes, aquatic habitat conditions, and biological integrity. In addition, human land-use impacts are factored into the conceptual model because they can alter habitat quality and can disrupt natural habitat forming processes. In this model (Figure S.1), aquatic habitat--both instream and riparian--is viewed as the link between watershed conditions and biologic responses. Based on this conceptual model, assessment of habitat loss and the resultant declines in salmonid populations can be conducted by relating current and historical (e.g., natural) habitat conditions to salmonid utilization, diversity, and abundance. In addition, assessing disrupted ecosystem functions and processes within the watershed can aid in identifying the causes of habitat change and the associated decline in biological integrity. In this same way, restoration, enhancement, and conservation projects can be identified and prioritized. A watershed assessment is primarily a landscape-scale evaluation of current watershed conditions and the associated hydrogeomorphic riverine processes. The watershed assessment conducted for this project focused on watershed processes that form and maintain salmonid habitat. Landscape metrics describing the level of human alteration of natural ecosystem attributes were used as indicators of water quality, hydrology, channel geomorphology, instream habitat, and biotic integrity. Ecological (watershed) processes are related to and can be predicted based on specific aspects of spatial pattern. This study evaluated the hydrologic regime, sediment delivery regime, and riparian condition of the sub-watersheds that comprise the upper Grays River watershed relative to their natural range of conditions. Analyses relied primarily on available geographic information system (GIS) data describing landscape characteristics such as climate, vegetation type and maturity, geology and soils, topography, land use, and road density. In addition to watershed-scale landscape characteristics, the study area was also evaluated on the riparian scale, with appropriate landscape variables analyzed within riparian buffers around each stream or river channel. Included in the overall watershed assessment are field habitat surveys and analyses of the physical and hydrological characteristics of primary chum and fall Chinook salmon spawning areas and spawning habitat availability and use. This assessment is a significant step in a comprehensive program to ensure the survival and recovery of Columbia River chum salmon in its most productive system and builds on existing recovery planning efforts for these ESA-listed salmonids within the Grays River and the lower Columbia River. This assessment also provides a basis for the recovery of other fish species in the Grays River, including coho salmon, winter steelhead, coastal cutthroat trout, and Pacific lamprey.

  17. Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from

    Energy Savers [EERE]

    Development | Department of Energy Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development Sage-Grouse and Wind Energy: Biology, Habits, and Potential Effects from Development PDF icon PNNL-18567.pdf More Documents & Publications EA-1772: Final Environmental Assessment EA-1772: Finding of No Significant Impact EIS-0521: Notice of Intent to Prepare an Environmental Impact

  18. Phytochrome from Green Plants: Properties and biological Function

    SciTech Connect (OSTI)

    Quail, Peter H.

    2014-07-25

    Plants constantly monitor the light environment for informational light signals used to direct adaptational responses to the prevailing conditions. One major such response, the Shade-Avaoidance Response (SAR), triggered when plants sense the presence of competing neighbors, results in enhanced channeling of photosynthetically-fixed carbon into stem elongation at the expense of deposition in reproductive tissues. This response has been selected against in many modern food crops to ensure maximum edible yield (e.g. seeds). Converse enhancement of the SAR, with consequent increased carbon channeling into vegetative cellulose, could contribute to the generation of crops with improved yield of tissues suitable for cellulosic biofuel production. The signal for this response is light enriched in far-red wavelengths. This signal is produced by sunlight filtered through, or reflected from, neighboring vegetation, as a result of preferential depletion of red photons through chlorophyll absorption. The plant phytochrome (phy) photoreceptor system (predominantly phyB) senses this signal through its capacity to switch reversibly, in milliseconds, between two molecular states: the biologically inactive Pr (red-light-absorbing) and biologically active Pfr (far-red-light-absorbing) conformers. The photoequilibrium established between these two conformers in light-grown plants is determined by the ratio of red-to-far-red wavelengths in the incoming signal. The levels of Pfr then dictate the recipient plants growth response: high levels suppress elongation growth; low levels promote elongation growth. Studies on seedling deetiolation have advanced our understanding considerably in recent years, of the mechanism by which the photoactivated phy molecule transduces its signal into cellular growth responses. The data show that a subfamily of phy-interacting bHLH transcription factors (PIFs) promote skotomorphogenic seedling development in post-germinative darkness, but that the phy Pfr conformer reverses this activity upon initial light exposure, inducing the switch to photomorphogenic development. This reversal involves light-triggered translocation of the photoactivated phy molecule into the nucleus where it interacts with PIF-family members, inducing rapid phosphorylation and degradation of the PIFs via the ubiquitin-proteasome system. This degradation in turn elicits rapid alterations in gene expression that drive the deetiolation transition. This project has made considerable progress in defining phy-PIF signaling activity in controlling the SAR. The biological functions of the multiple PIF-family members in controlling the SAR, including dissection of the relative contributions of the individual PIFs to this process, as well as to diurnal growth-control oscillations, have been investigated using higher-order pif-mutant combinations. Using microarray analysis of a quadruple pif mutant we have defined the shade-induced, PIF-regulated transcriptional network genome-wide. This has revealed that a dynamic antagonism between the phys and PIFs generates selective reciprocal responses during deetiolation and the SAR in a rapidly light-responsive transcriptional network. Using integrated RNA-seq and ChIP-seq analysis of higher order pif-mutant combinations, we have defined the direct gene-targets of PIF transcriptional regulation, and have obtained evidence that this regulation involves differential direct targeting of rapidly light-responsive genes by the individual PIF-family members. This project has provided significant advances in our understanding of the molecular mechanisms by which the phy-PIF photosensory signaling pathway regulates an important bioenergy-related plant response to the light environment. The identification of molecular targets in the primary transcriptional-regulatory circuitry of this pathway has the potential to enable genetic or reverse-genetic manipulation of the partitioning of carbon between reproductive and vegetative (cellulose-accumulating) tissue, toward enhanced bioenergy yield.

  19. KineticsFinal Report Cover Page Bakajin, O 59 BASIC BIOLOGICAL...

    Office of Scientific and Technical Information (OSTI)

    KineticsFinal Report Cover Page Bakajin, O 59 BASIC BIOLOGICAL SCIENCES; 42 ENGINEERING; CONSUMPTION RATES; DEAD TIME; DETECTION; DIFFUSION; DNA; ENERGY TRANSFER; FABRICATION;...

  20. High Performance Computational Biology: A Distributed computing Perspective (2010 JGI/ANL HPC Workshop)

    ScienceCinema (OSTI)

    Konerding, David [Google, Inc

    2011-06-08

    David Konerding from Google, Inc. gives a presentation on "High Performance Computational Biology: A Distributed Computing Perspective" at the JGI/Argonne HPC Workshop on January 26, 2010.

  1. Lensless Imaging of Whole Biological Cells with Soft X-Rays

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    whole yeast cells, achieving the highest resolution-11 to 13 nanometers (billionths of a meter)-ever obtained with this method for biological specimens. Their success indicates...

  2. Lensless Imaging of Whole Biological Cells with Soft X-Rays

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    capabilities in both biology and materials sciences. Piecing it all Together Lensless imaging uses a computer to decode light diffraction patterns that are captured by a...

  3. The OME Framework for genome-scale systems biology

    SciTech Connect (OSTI)

    Palsson, Bernhard O.; Ebrahim, Ali; Federowicz, Steve

    2014-12-19

    The life sciences are undergoing continuous and accelerating integration with computational and engineering sciences. The biology that many in the field have been trained on may be hardly recognizable in ten to twenty years. One of the major drivers for this transformation is the blistering pace of advancements in DNA sequencing and synthesis. These advances have resulted in unprecedented amounts of new data, information, and knowledge. Many software tools have been developed to deal with aspects of this transformation and each is sorely needed [1-3]. However, few of these tools have been forced to deal with the full complexity of genome-scale models along with high throughput genome- scale data. This particular situation represents a unique challenge, as it is simultaneously necessary to deal with the vast breadth of genome-scale models and the dizzying depth of high-throughput datasets. It has been observed time and again that as the pace of data generation continues to accelerate, the pace of analysis significantly lags behind [4]. It is also evident that, given the plethora of databases and software efforts [5-12], it is still a significant challenge to work with genome-scale metabolic models, let alone next-generation whole cell models [13-15]. We work at the forefront of model creation and systems scale data generation [16-18]. The OME Framework was borne out of a practical need to enable genome-scale modeling and data analysis under a unified framework to drive the next generation of genome-scale biological models. Here we present the OME Framework. It exists as a set of Python classes. However, we want to emphasize the importance of the underlying design as an addition to the discussions on specifications of a digital cell. A great deal of work and valuable progress has been made by a number of communities [13, 19-24] towards interchange formats and implementations designed to achieve similar goals. While many software tools exist for handling genome-scale metabolic models or for genome-scale data analysis, no implementations exist that explicitly handle data and models concurrently. The OME Framework structures data in a connected loop with models and the components those models are composed of. This results in the first full, practical implementation of a framework that can enable genome-scale design-build-test. Over the coming years many more software packages will be developed and tools will necessarily change. However, we hope that the underlying designs shared here can help to inform the design of future software.

  4. Method for in situ biological conversion of coal to methane

    DOE Patents [OSTI]

    Volkwein, Jon C. (Pittsburgh, PA)

    1995-01-01

    A method and apparatus are provided for the in situ biological conversion of coal to methane comprising culturing on a coal-containing substrate a consortium of microorganisms capable of degrading the coal into methane under suitable conditions. This consortium of microorganisms can be obtained from an underground cavity such as an abandoned mine which underwent a change from being supplied with sewage to where no sewage was present, since these conditions have favored the development of microorganisms capable of using coal as a carbon source and converting coal to methane. The consortium of microorganisms obtained from such abandoned coal mines can be isolated and introduced to hard-to-reach coal-containing substrates which lack such microorganisms and which would otherwise remain unrecoverable. The present invention comprises a significant advantage in that useable energy can be obtained from a number of abandoned mine sites or other areas wherein coal is no longer being recovered, and such energy can be obtained in a safe, efficient, and inexpensive manner.

  5. Effects of simulated rare earth recycling wastewaters on biological nitrification

    SciTech Connect (OSTI)

    Fujita, Yoshiko; Barnes, Joni; Eslamimanesh, Ali; Lencka, Malgorzata M.; Anderko, Andrzej; Riman, Richard E.; Navrotsky, Alexandra

    2015-07-16

    Current efforts to increase domestic availability of rare-earth element (REE) supplies by recycling and expanded ore processing efforts will result in increased generation of associated wastewaters. In some cases disposal to a sewage treatment plant may be favored but plant performance must be maintained. To assess the potential effects of such wastewaters on biological wastewater treatment, model nitrifying organisms Nitrosomonas europaea and Nitrobacter winogradskyi were exposed to simulated wastewaters containing varying levels of yttrium or europium (10, 50 and 100 ppm), and the REE extractant tributyl phosphate (TBP, at 0.1 g/L). Y and Eu additions above 10 ppm inhibited N. europaea activity, even when initially virtually all of the REE was insoluble. The provision of TBP together with Eu increased inhibition of nitrite production by the N. europaea, although TBP alone did not substantially alter nitrifying activity N. winogradskyi was more sensitive to the stimulated wastewaters, with even 10 ppm Eu or Y inducing significant inhibition, and a complete shutdown of nitrifying activity occurred in the presence of the TBP. To analyze the availability of REEs in aqueous solutions, REE solubility has been calculated using the previously developed MSE (Mixed-Solvent Electrolyte) thermodynamic model. The model calculations reveal a strong pH dependence of solubility, which is typically controlled by the precipitation of REE hydroxides but may also be influenced by the formation of a phosphate phase.

  6. Interaction of carbon nanohorns with plants: Uptake and biological effects

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lahiani, Mohamed H.; Chen, Jihua; Irin, Fahmida; Puretzky, Alexander A.; Green, Micah J.; Khodakovskaya, Mariya V.

    2014-10-07

    Single-Walled Carbon Nanohorns (SWCNHs) are a unique carbon-based nanomaterial with promising application in different fields including, medicine, genetic engineering and horticulture. Here, we investigated the biological response of six crop species (barley, corn, rice, soybean, switchgrass, tomato) and tobacco cell culture to the exposure of SWCNHs. We found that SWCNHs can activate seed germination of selected crops and enhance growth of different organs of corn, tomato, rice and soybean. At cellular level, growth of tobacco cells was increased in response to exposure of SWCNHs (78% increase compared to control). Uptake of SWCNHs by exposed crops and tobacco cells was confirmedmore » by transmission electron microscopy (TEM) and quantified by microwave induced heating (MIH) technique. At genetic level, SWCNHs were able to affect expression of a number of tomato genes that are involved in stress responses, cellular responses and metabolic processes. Our conclusion is that SWCNHs can be used as plant growth regulators and have the potential for plant-related applications.« less

  7. Bioforensics: Characterization of biological weapons agents by NanoSIMS

    SciTech Connect (OSTI)

    Weber, P K; Ghosal, S; Leighton, T J; Wheeler, K E; Hutcheon, I D

    2007-02-26

    The anthrax attacks of Fall 2001 highlight the need to develop forensic methods based on multiple identifiers to determine the origin of biological weapons agents. Genetic typing methods (i.e., DNA and RNA-based) provide one attribution technology, but genetic information alone is not usually sufficient to determine the provenance of the material. Non-genetic identifiers, including elemental and isotopic signatures, provide complementary information that can be used to identify the means, geographic location and date of production. Under LDRD funding, we have successfully developed the techniques necessary to perform bioforensic characterization with the NanoSIMS at the individual spore level. We have developed methods for elemental and isotopic characterization at the single spore scale. We have developed methods for analyzing spore sections to map elemental abundance within spores. We have developed rapid focused ion beam (FIB) sectioning techniques for spores to preserve elemental and structural integrity. And we have developed a high-resolution depth profiling method to characterize the elemental distribution in individual spores without sectioning. We used these newly developed methods to study the controls on elemental abundances in spores, characterize the elemental distribution of in spores, and to study elemental uptake by spores. Our work under this LDRD project attracted FBI and DHS funding for applied purposes.

  8. Combinatorial synthesis and screening of non-biological polymers

    DOE Patents [OSTI]

    Schultz, Peter G.; Xiang, Xiao-Dong; Goldwasser, Isy; Briceno, Gabriel; Sun, Xiao-Dong; Wang, Kai-An

    2006-04-25

    Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, non-biological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties.

  9. Methods of biological dosimetry employing chromosome-specific staining

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel

    2000-01-01

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome. Probes and test kits are provided for use in tumor cytogenetics, in the detection of disease related loci, in analysis of structural abnormalities, such as translocations, and for biological dosimetry. Further, methods and prenatal test kits are provided to stain targeted chromosomal material of fetal cells, including fetal cells obtained from maternal blood. Still further, the invention provides for automated means to detect and analyse chromosomal abnormalities.

  10. Autonomous observations of the ocean biological carbon pump

    SciTech Connect (OSTI)

    Bishop, James K.B.

    2009-03-01

    Prediction of the substantial biologically mediated carbon flows in a rapidly changing and acidifying ocean requires model simulations informed by observations of key carbon cycle processes on the appropriate space and time scales. From 2000 to 2004, the National Oceanographic Partnership Program (NOPP) supported the development of the first low-cost fully-autonomous ocean profiling Carbon Explorers that demonstrated that year-round real-time observations of particulate organic carbon (POC) concentration and sedimentation could be achieved in the world's ocean. NOPP also initiated the development of a sensor for particulate inorganic carbon (PIC) suitable for operational deployment across all oceanographic platforms. As a result, PIC profile characterization that once required shipboard sample collection and shipboard or shore based laboratory analysis, is now possible to full ocean depth in real time using a 0.2W sensor operating at 24 Hz. NOPP developments further spawned US DOE support to develop the Carbon Flux Explorer, a free-vehicle capable of following hourly variations of particulate inorganic and organic carbon sedimentation from near surface to kilometer depths for seasons to years and capable of relaying contemporaneous observations via satellite. We have demonstrated the feasibility of real time - low cost carbon observations which are of fundamental value to carbon prediction and when further developed, will lead to a fully enhanced global carbon observatory capable of real time assessment of the ocean carbon sink, a needed constraint for assessment of carbon management policies on a global scale.

  11. Nonlinear, noniterative, single-distance phase retrieval and developmental biology

    SciTech Connect (OSTI)

    Moosmann, Julian; Altapova, Venera; Haenschke, Daniel; Hofmann, Ralf; Baumbach, Tilo

    2012-05-17

    For coherent X-ray imaging, based on phase contrast through free-space Fresnel propagation, we discuss two noniterative, nonlinear approaches to the phase-retrieval problem from a single-distance intensity map of a pure-phase object. On one hand, a perturbative set-up is proposed where nonlinear corrections to the linearized transport-of-intensity situation are expanded in powers of the object-detector distance z and are evaluated in terms of the linear estimate. On the other hand, a nonperturbative projection algorithm, which is based on the (linear and local) contrast-transfer function (CTF), works with an effective phase in Fourier space. This effective phase obeys a modified CTF relation between intensity contrast at z > 0 and phase contrast at z= 0: Unphysical singularities of the local CTF model are cut off to yield 'quasiparticles' in analogy to the theory of the Fermi liquid. By identifying the positions of the zeros of the Fourier transformed intensity contrast as order parameters for the dynamical breaking of scaling symmetry we investigate the phase structure of the forward-propagation problem when interpreted as a statistical system. Results justify the quasiparticle approach for a wide range of intermediary phase variations. The latter algorithm is applied to data from biological samples recorded at the beamlines TopoTomo and ID19 at ANKA and ESRF, respectively.

  12. Interaction of carbon nanohorns with plants: Uptake and biological effects

    SciTech Connect (OSTI)

    Lahiani, Mohamed H.; Chen, Jihua; Irin, Fahmida; Puretzky, Alexander A.; Green, Micah J.; Khodakovskaya, Mariya V.

    2014-10-07

    Single-Walled Carbon Nanohorns (SWCNHs) are a unique carbon-based nanomaterial with promising application in different fields including, medicine, genetic engineering and horticulture. Here, we investigated the biological response of six crop species (barley, corn, rice, soybean, switchgrass, tomato) and tobacco cell culture to the exposure of SWCNHs. We found that SWCNHs can activate seed germination of selected crops and enhance growth of different organs of corn, tomato, rice and soybean. At cellular level, growth of tobacco cells was increased in response to exposure of SWCNHs (78% increase compared to control). Uptake of SWCNHs by exposed crops and tobacco cells was confirmed by transmission electron microscopy (TEM) and quantified by microwave induced heating (MIH) technique. At genetic level, SWCNHs were able to affect expression of a number of tomato genes that are involved in stress responses, cellular responses and metabolic processes. Our conclusion is that SWCNHs can be used as plant growth regulators and have the potential for plant-related applications.

  13. A network biology approach to denitrification in Pseudomonas aeruginosa

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Arat, Seda; Bullerjahn, George S.; Laubenbacher, Reinhard

    2015-02-23

    Pseudomonas aeruginosa is a metabolically flexible member of the Gammaproteobacteria. Under anaerobic conditions and the presence of nitrate, P. aeruginosa can perform (complete) denitrification, a respiratory process of dissimilatory nitrate reduction to nitrogen gas via nitrite (NO₂), nitric oxide (NO) and nitrous oxide (N₂O). This study focuses on understanding the influence of environmental conditions on bacterial denitrification performance, using a mathematical model of a metabolic network in P. aeruginosa. To our knowledge, this is the first mathematical model of denitrification for this bacterium. Analysis of the long-term behavior of the network under changing concentration levels of oxygen (O₂), nitrate (NO₃),more » and phosphate (PO₄) suggests that PO₄ concentration strongly affects denitrification performance. The model provides three predictions on denitrification activity of P. aeruginosa under various environmental conditions, and these predictions are either experimentally validated or supported by pertinent biological literature. One motivation for this study is to capture the effect of PO₄ on a denitrification metabolic network of P. aeruginosa in order to shed light on mechanisms for greenhouse gas N₂O accumulation during seasonal oxygen depletion in aquatic environments such as Lake Erie (Laurentian Great Lakes, USA). Simulating the microbial production of greenhouse gases in anaerobic aquatic systems such as Lake Erie allows a deeper understanding of the contributing environmental effects that will inform studies on, and remediation strategies for, other hypoxic sites worldwide.« less

  14. Compact Beta Particle/Positron Imager for Plant Biology

    SciTech Connect (OSTI)

    Weisenberger, Andrew; Lee, Seung Joon; McKisson, John; Xi, Wenze; Zorn, Carl; Stolin, Alexander; Majewski, Stan; Majewski, Stanislaw; Howell, Calvin; Crowell, Alec

    2011-06-01

    The 11CO2 tracer is used to facilitate plant biology research towards optimization of plant productivity, biofuel development and carbon sequestration in biomass. Positron emission tomography (PET) imaging has been used to study carbon transport in live plants using 11CO2. Plants typically have very thin leaves resulting in little medium for the emitted positrons to undergo an annihilation event. For the emitted positron from 11C decay approximately 1mm of water equivalent material is needed for positron annihilation. Thus most of the positrons do not annihilate inside the leaf, resulting in limited sensitivity for PET imaging. To address this problem we have developed a compact beta-positive beta-minus particle (BPBM) imager for 11CO2 leaf imaging. The detector is based on a Hamamatsu H8500 position sensitive photomultiplier tube optically coupled via optical grease and a 3mm thick glass plate to a 0.5mm thick Eljin EJ-212 plastic scintillator. The detector is equipped with a flexible arm to allow its placement and orientation on the leaf of the plant of interest while maintaining the leaf's original orientation. We are planning to utilize the imaging device at the Duke University Phytotron to investigate dynamic carbon transport differences between invasive and native species.

  15. Effects of simulated rare earth recycling wastewaters on biological nitrification

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Fujita, Yoshiko; Barnes, Joni; Eslamimanesh, Ali; Lencka, Malgorzata M.; Anderko, Andrzej; Riman, Richard E.; Navrotsky, Alexandra

    2015-07-16

    Current efforts to increase domestic availability of rare-earth element (REE) supplies by recycling and expanded ore processing efforts will result in increased generation of associated wastewaters. In some cases disposal to a sewage treatment plant may be favored but plant performance must be maintained. To assess the potential effects of such wastewaters on biological wastewater treatment, model nitrifying organisms Nitrosomonas europaea and Nitrobacter winogradskyi were exposed to simulated wastewaters containing varying levels of yttrium or europium (10, 50 and 100 ppm), and the REE extractant tributyl phosphate (TBP, at 0.1 g/L). Y and Eu additions above 10 ppm inhibited N.more » europaea activity, even when initially virtually all of the REE was insoluble. The provision of TBP together with Eu increased inhibition of nitrite production by the N. europaea, although TBP alone did not substantially alter nitrifying activity N. winogradskyi was more sensitive to the stimulated wastewaters, with even 10 ppm Eu or Y inducing significant inhibition, and a complete shutdown of nitrifying activity occurred in the presence of the TBP. To analyze the availability of REEs in aqueous solutions, REE solubility has been calculated using the previously developed MSE (Mixed-Solvent Electrolyte) thermodynamic model. The model calculations reveal a strong pH dependence of solubility, which is typically controlled by the precipitation of REE hydroxides but may also be influenced by the formation of a phosphate phase.« less

  16. Matrix Effects in Biological Mass Spectrometry Imaging: Identification and Compensation

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Stevens, Susan; Stenzel-Poore, Mary; Laskin, Julia

    2014-07-21

    Matrix effects in mass spectrometry imaging (MSI) may affect the observed molecular distribution in chemical and biological systems. In this study, we introduce an experimental approach that efficiently compensates for matrix effects in nanospray desorption electrospray ionization (nano-DESI) MSI without introducing any complexity into the experimental protocol. We demonstrate compensation for matrix effects in nano-DESI MSI of phosphatidylcholine (PC) in normal and ischemic mouse brain tissue by doping the nano-DESI solvent with PC standards. Specifically, we use mouse brain tissue of a middle cerebral artery occlusion (MCAO) stroke model with an ischemic region localized to one hemisphere of the brain. Due to similar suppression in ionization of endogenous PC molecules extracted from the tissue and PC standards added to the solvent, matrix effects are eliminated by normalizing the intensity of the sodium and potassium adducts of endogenous PC to the intensity of the corresponding adduct of the PC standard. This approach efficiently compensates for signal variations resulting from differences in the local concentrations of sodium and potassium in tissue sections and from the complexity of the extracted analyte mixture derived from local variations in molecular composition.

  17. Biologically-based signal processing system applied to noise removal for signal extraction

    DOE Patents [OSTI]

    Fu, Chi Yung; Petrich, Loren I.

    2004-07-13

    The method and system described herein use a biologically-based signal processing system for noise removal for signal extraction. A wavelet transform may be used in conjunction with a neural network to imitate a biological system. The neural network may be trained using ideal data derived from physical principles or noiseless signals to determine to remove noise from the signal.

  18. Chemically-functionalized microcantilevers for detection of chemical, biological and explosive material

    DOE Patents [OSTI]

    Pinnaduwage, Lal A [Knoxville, TN; Thundat, Thomas G [Knoxville, TN; Brown, Gilbert M [Knoxville, TN; Hawk, John Eric [Olive Branch, MS; Boiadjiev, Vassil I [Knoxville, TN

    2007-04-24

    A chemically functionalized cantilever system has a cantilever coated on one side thereof with a reagent or biological species which binds to an analyte. The system is of particular value when the analyte is a toxic chemical biological warfare agent or an explosive.

  19. Rotating biological contactors: Wastewater treatment. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect (OSTI)

    1995-10-01

    The bibliography contains citations concerning wastewater treatment using rotating biological contactors (RBC). Citations focus on reaction kinetics, operational modeling, and removal efficiencies. Biological oxygen demand (BOD) and nitrogen removal are discussed. Citations examine performance of RBCs in industrial and municipal applications. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  20. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.

    DOE Patents [OSTI]

    Alfano, Robert R.; Wang, Wubao

    2003-05-06

    A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. A low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuring the characteristic excitation Raman lines of blood glucose, cholesterol, serum glutamic oxalacetic transaminase (SGOT)/serum glutamic pyruvic transaminase (SGPT), tissues and other corresponding Raman-active body constituents, respectively.

  1. Detection and treatment of chemical weapons and/or biological pathogens

    DOE Patents [OSTI]

    Mariella Jr., Raymond P.

    2004-09-07

    A system for detection and treatment of chemical weapons and/or biological pathogens uses a detector system, an electrostatic precipitator or scrubber, a circulation system, and a control. The precipitator or scrubber is activated in response to a signal from the detector upon the detection of chemical weapons and/or biological pathogens.

  2. 2012 PLANT MOLECULAR BIOLOGY GORDON RESEARCH CONFERENCE, JULY 15-20, 2012

    SciTech Connect (OSTI)

    Sussman, Michael

    2013-07-20

    The 2012 Gordon Conference on Plant Molecular Biology will present cutting-edge research on molecular aspects of plant growth and development, with particular emphasis on recent discoveries in molecular mechanisms involved with plant signaling systems. The Conference will feature a wide range of topics in plant molecular biology including hormone receptors and early events in hormone signaling, plant perception of and response to plant pathogen and symbionts, as well as technological and biological aspects of epigenomics particularly as it relates to signaling systems that regulate plant growth and development. Genomic approaches to plant signaling will be emphasized, including genomic profiling technologies for quantifying various biological subsystems, such as the epigenome, transcriptome, phosphorylome, and metabolome. The meeting will include an important session devoted to answering the question, "What are the biological and technological limits of plant breeding/genetics, and how can they be solved"?

  3. 2012 CELLULAR & MOLECULAR FUNGAL BIOLOGY GORDON RESEARCH CONFERENCE, JUNE 17 - 22, 2012

    SciTech Connect (OSTI)

    Judith Berman

    2012-06-22

    The Gordon Research Conference on CELLULAR & MOLECULAR FUNGAL BIOLOGY was held at Holderness School, Holderness New Hampshire, June 17 - 22, 2012. The 2012 Gordon Conference on Cellular and Molecular Fungal Biology (CMFB) will present the latest, cutting-edge research on the exciting and growing field of molecular and cellular aspects of fungal biology. Topics will range from yeast to filamentous fungi, from model systems to economically important organisms, and from saprophytes and commensals to pathogens of plants and animals. The CMFB conference will feature a wide range of topics including systems biology, cell biology and morphogenesis, organismal interactions, genome organisation and regulation, pathogenesis, energy metabolism, biomass production and population genomics. The Conference was well-attended with 136 participants. Gordon Research Conferences does not permit publication of meeting proceedings.

  4. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Biedermann, Laura Butler (2) Ohta, Taisuke (2) Pan, Wei (2) Friedmann, Thomas A. (1) ... June 2008 Enabling graphene nanoelectronics. Pan, Wei ; Ohta, Taisuke ; Biedermann, Laura ...

  5. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Bates, Frank (1) Brown, Gordon (1) Crabtree, George (1) French, Roger (1) Greene, Laura ... William ; Bates, Frank ; Brown, Gordon ; French, Roger ; Greene, Laura ; Hemminger, John ...

  6. 2013 Federal Energy and Water Management Award Winners Chris...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Chris Manis, Randy Monohan, Laura Nelson, Mark Rodriguez, and Mick Wasco 2013 Federal Energy and Water Management Award Winners Chris Manis, Randy Monohan, Laura Nelson, Mark ...

  7. Browse by Discipline -- E-print Network Subject Pathways: Power

    Office of Scientific and Technical Information (OSTI)

    Transmission, Distribution and Plants -- Energy, science, and technology for the research community -- hosted by the Office of Scientific and Technical Information, U.S. Department of Energy K L M N O P Q R S T U V W X Y Z Jackman, Todd (Todd Jackman) - Biology Department, Villanova University Jackson, Don (Don Jackson) - Department of Ecology and Evolutionary Biology, University of Toronto Jackson, Robert B. (Robert B. Jackson) - Department of Biology & Nicholas School of the

  8. Frontiers of Plant Cell Biology: Signals and Pathways, System-Based Approaches 22nd Symposium in Plant Biology (University of California-Riverside)

    SciTech Connect (OSTI)

    Minorsky, Peter V.

    2003-06-01

    The symposium ''Frontiers of Plant Cell Biology: Signals and Pathways, Systems-Based Approaches'' was held January 15-18, 2003 at the Riverside Convention Center in Riverside, California. The host organization for the symposium was the Center for Plant Cell Biology (CEPCEB) at the University of California, Riverside (UCR). The meeting, focusing on systems-based approaches to plant cell biology research, was the first of this kind in the field of plant biology. The speakers and nearly 100 posters placed emphasis on recent developments in plant cellular biology and molecular genetics, particularly those employing emerging genomic tools, thereby sharing the most current knowledge in the field and stimulating future advances. In attendance were many well-established scientists and young investigators who approach plant cell biology from different but complementary conceptual and technical perspectives. Indeed, many disciplines are converging in the field of cell biology, producing synergies that will enable plant scientists to determine the function of gene products in the context of living cells in whole organisms. New, cross-disciplinary collaborations, as well as the involvement of computer scientists and chemists in plant biology research, are likely additional outcomes of the symposium. The program included 39 invited session speakers and workshop/panel speakers. Sessions were convened on the following themes: Cell-Cell Communication; Protein Trafficking; Cell Surface, Extracellular Matrix and Cell Wall; Signal Transduction; Signal Transduction and Proteosome; and Systems-Based Approaches to Plant Cell Biology. Workshops on Chemical Genetics and Visual Microscopy were also presented. Abstracts from each of the speaker presentations, as well as the posters presented at the meeting were published in a program booklet given to the 239 faculty members, researchers, postdoctoral scientists and graduate students in attendance. The booklet thus serves as a reference for symposium attendees to locate additional information about a topic of their particular interest and to contact other investigators. In addition, an article reviewing the symposium by science writer Peter V. Minorsky appeared in the June 2003 issue of Plant Physiology, a special issue devoted to systems-based approaches in the study of the model plant Arabidopsis (article submitted as part of this Final Technical Report).

  9. SREL Reprint #3214

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4 Dispersal via stream corridors structures populations of the endangered St. Francis' satyr butterfly (Neonympha mitchellii francisci) Laura V. Milko1, Nick M. Haddad1, and Stacey L. Lance2 1Department of Biology, North Carolina State University, Raleigh, NC 27695, USA 2Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA Abstract: Habitat fragmentation may reduce gene flow and population viability of rare species. We tested whether riparian corridors enhanced gene

  10. Amchitka Island, Alaska, Biological Monitoring Report 2011 Sampling Results

    SciTech Connect (OSTI)

    2013-09-01

    The Long-Term Surveillance and Maintenance (LTS&M) Plan for the U.S. Department of Energy (DOE) Office of Legacy Management (LM) Amchitka Island sites describes how LM plans to conduct its mission to protect human health and the environment at the three nuclear test sites located on Amchitka Island, Alaska. Amchitka Island, near the western end of the Aleutian Islands, is approximately 1,340 miles west-southwest of Anchorage, Alaska. Amchitka is part of the Aleutian Island Unit of the Alaska Maritime National Wildlife Refuge, which is administered by the U.S. Fish and Wildlife Service (USFWS). Since World War II, Amchitka has been used by multiple U.S. government agencies for various military and research activities. From 1943 to 1950, it was used as a forward air base for the U.S. Armed Forces. During the middle 1960s and early 1970s, the U.S. Department of Defense (DOD) and the U.S. Atomic Energy Commission (AEC) used a portion of the island as a site for underground nuclear tests. During the late 1980s and early 1990s, the U.S. Navy constructed and operated a radar station on the island. Three underground nuclear tests were conducted on Amchitka Island. DOD, in conjunction with AEC, conducted the first nuclear test (named Long Shot) in 1965 to provide data that would improve the United States' capability of detecting underground nuclear explosions. The second nuclear test (Milrow) was a weapons-related test conducted by AEC in 1969 as a means to study the feasibility of detonating a much larger device. Cannikin, the third nuclear test on Amchitka, was a weapons-related test detonated on November 6, 1971. With the exception of small concentrations of tritium detected in surface water shortly after the Long Shot test, radioactive fission products from the tests remain in the subsurface at each test location As a continuation of the environmental monitoring that has taken place on Amchitka Island since before 1965, LM in the summer of 2011 collected biological and seawater samples from the marine and terrestrial environment of Amchitka Island adjacent to the three detonation sites and at a background or reference site, Adak Island, 180 miles to the east. Consistent with the goals of the Amchitka LTS&M Plan, four data quality objectives (DQOs) were developed for the 2011 sampling event.

  11. Enabling a Systems Biology Knowledgebase with Gaggle and Firegoose

    SciTech Connect (OSTI)

    Baliga, Nitin S.

    2014-12-12

    The overall goal of this project was to extend the existing Gaggle and Firegoose systems to develop an open-source technology that runs over the web and links desktop applications with many databases and software applications. This technology would enable researchers to incorporate workflows for data analysis that can be executed from this interface to other online applications. The four specific aims were to (1) provide one-click mapping of genes, proteins, and complexes across databases and species; (2) enable multiple simultaneous workflows; (3) expand sophisticated data analysis for online resources; and enhance open-source development of the Gaggle-Firegoose infrastructure. Gaggle is an open-source Java software system that integrates existing bioinformatics programs and data sources into a user-friendly, extensible environment to allow interactive exploration, visualization, and analysis of systems biology data. Firegoose is an extension to the Mozilla Firefox web browser that enables data transfer between websites and desktop tools including Gaggle. In the last phase of this funding period, we have made substantial progress on development and application of the Gaggle integration framework. We implemented the workspace to the Network Portal. Users can capture data from Firegoose and save them to the workspace. Users can create workflows to start multiple software components programmatically and pass data between them. Results of analysis can be saved to the cloud so that they can be easily restored on any machine. We also developed the Gaggle Chrome Goose, a plugin for the Google Chrome browser in tandem with an opencpu server in the Amazon EC2 cloud. This allows users to interactively perform data analysis on a single web page using the R packages deployed on the opencpu server. The cloud-based framework facilitates collaboration between researchers from multiple organizations. We have made a number of enhancements to the cmonkey2 application to enable and improve the integration within different environments, and we have created a new tools pipeline for generating EGRIN2 models in a largely automated way.

  12. Procedure for developing biological input for the design, location, or modification of water-intake structures

    SciTech Connect (OSTI)

    Neitzel, D.A.; McKenzie, D.H.

    1981-12-01

    To minimize adverse impact on aquatic ecosystems resulting from the operation of water intake structures, design engineers must have relevant information on the behavior, physiology and ecology of local fish and shellfish. Identification of stimulus/response relationships and the environmental factors that influence them is the first step in incorporating biological information in the design, location or modification of water intake structures. A procedure is presented in this document for providing biological input to engineers who are designing, locating or modifying a water intake structure. The authors discuss sources of stimuli at water intakes, historical approaches in assessing potential/actual impact and review biological information needed for intake design.

  13. Interdisciplinary Dialogue for Education, Collaboration, and Innovation: Intelligent Biology and Medicine In and Beyond 2013

    SciTech Connect (OSTI)

    Zhang, Bing; Huang, Yufei; McDermott, Jason E.; Posey, Rebecca H.; Xu, Hua; Zhao, Zhongming

    2013-12-09

    The 2013 International Conference on Intelligent Biology and Medicine (ICIBM 2013) was held on August 11-13, 2013 in Nashville, Tennessee, USA. The conference included six scientific sessions, two tutorial sessions, one workshop, two poster sessions, and four keynote presentations that covered cutting-edge research topics in bioinformatics, systems biology, computational medicine, and intelligent computing. Here, we present a summary of the conference and an editorial report of the supplements to BMC Genomics and BMC Systems Biology that include 19 research papers selected from ICIBM 2013.

  14. Lensless Imaging of Whole Biological Cells with Soft X-Rays

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lensless Imaging of Whole Biological Cells with Soft X-Rays Lensless Imaging of Whole Biological Cells with Soft X-Rays Print Wednesday, 26 May 2010 00:00 A team of scientists has used x-ray diffraction microscopy at ALS Beamline 9.0.1 to make images of whole yeast cells, achieving the highest resolution-11 to 13 nanometers (billionths of a meter)-ever obtained with this method for biological specimens. Their success indicates that full 3-D tomography of whole cells at equivalent resolution

  15. DOE Systems Biology Knowledgebase (KBASE) (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    ScienceCinema (OSTI)

    Arkin, Adam [LBNL

    2013-01-15

    Adam Arkin from Berkeley Lab on the DOE Systems Biology Knowledgebase (KBASE) at the 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  16. Information resources and the correlation of response patterns between biological end points

    SciTech Connect (OSTI)

    Malling, H.V.; Wassom, J.S.

    1990-12-31

    This paper focuses on the analysis of information for mutagenesis, a biological end point that is important in the overall process of assessing possible adverse health effects from chemical exposure. 17 refs.

  17. Building the DOE Systems Biology Knowledgebase (KBase) ( 7th Annual SFAF Meeting, 2012)

    ScienceCinema (OSTI)

    Brettin, Tom [Oak Ridge National Laboratory

    2013-03-22

    Tom Brettin on "Building the DOE Systems Biology Knowledgebase (KBase)" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  18. DOE Systems Biology Knowledgebase (KBASE) (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)

    SciTech Connect (OSTI)

    Arkin, Adam [LBNL] [LBNL

    2012-03-21

    Adam Arkin from Berkeley Lab on the DOE Systems Biology Knowledgebase (KBASE) at the 7th Annual Genomics of Energy & Environment Meeting on March 21, 2012 in Walnut Creek, Calif

  19. Lensless Imaging of Whole Biological Cells with Soft X-Rays

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lensless Imaging of Whole Biological Cells with Soft X-Rays Print A team of scientists has used x-ray diffraction microscopy at ALS Beamline 9.0.1 to make images of whole yeast...

  20. Lensless Imaging of Whole Biological Cells with Soft X-Rays

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Whole Biological Cells with Soft X-Rays Print A team of scientists has used x-ray diffraction microscopy at ALS Beamline 9.0.1 to make images of whole yeast cells, achieving the...