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Sample records for involvement involvement involvement

  1. Commitment to Public Involvement

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Create a Sustainable Future Commitment to Public Involvement Commitment to Public Involvement LANL is committed to our neighbors August 1, 2013 Lab Director McMillan talks with...

  2. TPA Public Involvement Survey

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hanford Tri-Party Agencies' Annual Public Involvement Survey Activities for Calendar Year 2008 Nolan Curtis Washington State Department of Ecology Nuclear Waste Program August 5,...

  3. Commitment to Public Involvement

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Create a Sustainable Future » Commitment to Public Involvement Commitment to Public Involvement LANL is committed to our neighbors August 1, 2013 Lab Director McMillan talks with Lab historian Ellen McGhee during Lab tour Lab Director McMillan talks with Lab historian Ellen McGhee during Lab tour RELATED IMAGES http://farm4.staticflickr.com/3815/9442115459_390e5bc841_t.jpg Enlarge http://farm8.staticflickr.com/7111/7650961650_ed1571174f_t.jpg Enlarge

  4. Involvement and Communication Committee.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public Involvement and Communication Committee Chair 1 : Liz Mattson 4 , Member Vice Chair 1 : Shannon Cram, Member Committee Members 2 : Shelley Cimon Liz Mattson Alissa Cordner Ken Niles Shannon Cram Ed Pacheco Rob Davis Dan Serres Tom Galioto Jean Vanni Floyd Hodges Steve White Paige Knight Unofficial Committee Members or Other Interested Parties 3 : Earl Fordham Gerry Pollet Paige Knight Steve Hudson 4 Susan Leckband 4 Facilitator: Cathy McCague 4 Agency & Technical Support: Kris Holmes

  5. Public Involvement and Communications Committee:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    purposes only 09.08.15 Excerpt from the HAB Process Manual January 2012 Public Involvement and Communications Committee: Develops Board Advice for the TPA agencies on the...

  6. Public Involvement Committee - Transcribed Flipcharts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    September 3, 2014 Public Involvement Opportunities * Review of PI materials and agency websites, listservs (use of and purpose of notifications). * CD process - approach to PI...

  7. Public Involvement Committee - Transcribed Flipcharts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    education materials, including web materials * Evaluate effectiveness of TPA public involvement activities. Page 5 FY2015 Work Plan Ideas * Share innovative approaches to public...

  8. Public Involvment Plan - Rifle, Colorado

    Office of Legacy Management (LM)

    4-TAR MAC-GWRIF 7.1 UMTRA Ground Water Project Public Involvement Plan for the Environmental Assessment of Ground Water Compliance at the New and Old Rifle, Colorado, Uranium Mill Tailings Sites May 1999 Prepared by U.S. Department of Energy Grand Junction Office Grand Junction, Colorado Work performed under DOE Contract No. DE-AC13-96GJ87335 Public Involvement Plan for the Rifle UMTRA Sites Page 2 Introduction This Public Involvement Plan is tiered to the Uranium Mill Tailings Remedial Action

  9. Appendix V Public Involvement Plan

    Office of Legacy Management (LM)

    V Public Involvement Plan Revision No.: 6 February 2008 Federal Facility Agreement and Consent Order (FFACO) FFACO, Appendix V February 2008 i FFACO Public Involvement Plan U.S. Department of Energy National Nuclear Security Administration Nevada Site Office Las Vegas, Nevada U.S. Department of Defense Defense Threat Reduction Agency Detachment 1, Nevada Operations Mercury, Nevada U.S. Department of Energy Office of Legacy Management Grand Junction, Colorado FFACO, Appendix V February 2008 ii

  10. Public Involvement Committee - Transcribed Flipcharts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    December 10, 2013 100-F 1. Public involvement/mtg in March - How to sequence Board Review with public comment period * Path 1: Could request comment period extension * Path 2: Could request HAB comments considered post comment period * If comment period starts in March, HAB could request to comment in May (advice) Page 1 What might be topics for policy-level discussions? What might be ways, other than meetings, to solicit involvement? What might be useful to present at March Board mtg? Page 2

  11. HOW YOU CAN BE INVOLVED

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    HOW YOU CAN BE INVOLVED www.youtube.com/hanfordsite www.twitter.com/hanfordsite www.facebook.com/hanfordsite The Hanford Site sits on 586-square-miles of shrub-steppe desert in southeastern Washington state. The site was created in 1943 as part of the Manhattan Project to produce plutonium for nuclear weapons. Between 1943 and 1963, nine nuclear reactors were built along the banks of the Columbia River. By 1988, all nine reactors were shut down. The weapons material production mission ended in

  12. Public Involvement and Communications Committee Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8, 2012 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT & COMMUNICATIONS COMMITTEE MEETING February 8, 2012 Richland, WA Topics in this Meeting Summary Welcome and Introductions .......................................................................................................................... 1 Update on Timely Public Involvement Topics ............................................................................................. 1 Public Involvement for Technical

  13. DECONTAMINATION DRESSDOWN AT A TRANSPORTATION ACCIDENT INVOLVING...

    Office of Environmental Management (EM)

    Video User' s Guide DECONTAMINATION DRESSDOWN AT A TRANSPORTATION ACCIDENT INVOLVING ... related to emergency response to a transportation accident involving radioactive material. ...

  14. Community Involvement | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Involvement Community Involvement Y-12 is committed to keeping the community informed in areas of operations, environmental concerns, safety and emergency preparedness. To...

  15. Paducah WDA CERCLA Process & Public Involvement

    Broader source: Energy.gov [DOE]

    DOE is committed to fostering meaningful community involvement in environmental remediation decision making at the site.

  16. DOE - NNSA/NFO -- EM Public Involvement

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public Involvement NNSA/NFO Language Options U.S. DOE/NNSA - Nevada Field Office Click to subscribe to NNSS News Public Involvement Public Involvement Outreach Photo An integral component of the Environmental Management Program is the public involvement function. Communication with the public is an essential element in successfully conducting Environmental Management work. Public involvement initiatives include developing and distributing fact sheets, publications, and exhibits as well as

  17. EMERGENCY RESPONSE TO A TRANSPORTATION ACCIDENT INVOLVING RADIOACTIVE...

    Office of Environmental Management (EM)

    ransportation ransportation ransportation ransportation Accident Involving Radioactive Material Accident Involving Radioactive Material Accident Involving Radioactive ...

  18. Public Involvement Plan | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Public Involvement Plan Public Involvement Plan Every three years, the Public Involvement Plan is updated, prepared, and published by the U.S. Department of Energy Oak Ridge Office of Environmental Management (DOE-OREM) to communicate to you, as a member of the public, the opportunities you have to participate in decisions and information exchanges regarding the remediation of contaminated areas on the Oak Ridge Site. DOE is committed to fostering meaningful public involvement in all aspects of

  19. Public Involvement and Communications Committee Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    11, 2011 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT & COMMUNICATIONS COMMITTEE MEETING October 11, 2011 Richland, WA Topics in this Meeting Summary Welcome and Introductions ............................................................................................................ 1 Draft Advice - Draft Hanford Public Involvement Plan ................................................................ 1 Public Meetings - Draft Hanford Public Involvement Plan

  20. Public Involvement and Communications Committee Summaries - Hanford...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    05, 2012 Final Attachment 1: Tri-Party Agreement Agencies - Public Involvement Calendar Fiscal Year 2012 - not available Attachment 2: PIC Meeting Transcribed Flip Chart Notes...

  1. Community Involvement and Making a Difference

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2016 all issues All Issues submit Community Involvement and Making a Difference A personal message from Kurt Steinhaus, Community Programs Director, Los Alamos National...

  2. Public Involvement and Communications Committee Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    November 2, 2011 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT & COMMUNICATIONS COMMITTEE MEETING November 2, 2011 Kennewick, WA Topics in this Meeting Summary...

  3. Central Plateau Principles Public Involvement Advice DETAILED...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    v0, 12914 Central Plateau Principles Public Involvement Advice DETAILED BACKGROUND Cleanup of Hanford's Central Plateau is expected to take another four decades or longer, and...

  4. Public Involvement and Communications Committee Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    said he believes the heart of the Plan is in pages 7 to 11. He said the section uses language that is frequently used by the Board, including public involvement for better long...

  5. Public Involvement and Communications Committee Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    January 13, 2011 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT & COMMUNICATIONS COMMITTEE MEETING January 13, 2011 Richland, WA Topics in this Meeting Summary Welcome and Introductions ............................................................................................................ 1 State of the Site Meetings ............................................................................................................... 2 Open Government Plan Advice

  6. Strategic Public Involvement Products For Review

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public Involvement Products For Review PIC Discussion September 4, 2013 1 This list is from July 7, 2010 before we had decided on writing advice and is purely for reference for...

  7. Decontamination Dressdown at a Transportation Accident Involving

    Office of Environmental Management (EM)

    Radioactive Material | Department of Energy Decontamination Dressdown at a Transportation Accident Involving Radioactive Material Decontamination Dressdown at a Transportation Accident Involving Radioactive Material The purpose of this User's Guide is to provide instructors with an overview of the key points covered in the video. The Student Handout portion of this Guide is designed to assist the instructor in reviewing those points with students. The Student Handout should be distributed to

  8. A Good Neighbor: Community Involvement | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Good Neighbor: Community Involvement At NREL, we are dedicated environmental stewards and are committed to making positive contributions in our community. NREL's main campus is a model for environmental protection and sustainable building practices. Photo of a volunteer at the Foothills Animal Shelter. Charitable Contributions Employees are very involved in the community, giving their time, money, and energy. As staff levels have grown, so have contributions, making NREL Jefferson County's

  9. FOIASI - Special Inquiry Review of Allegations Involving

    Energy Savers [EERE]

    PotentialMisconduct by a Senior Office of Environmental Management Official | Department of Energy FOIASI - Special Inquiry Review of Allegations Involving PotentialMisconduct by a Senior Office of Environmental Management Official FOIASI - Special Inquiry Review of Allegations Involving PotentialMisconduct by a Senior Office of Environmental Management Official In September 2009, the Office of Inspector General (OIG) received multiple allegations concerning improprieties by a senior

  10. Manager`s views of public involvement

    SciTech Connect (OSTI)

    Branch, K.M.; Heerwagen, J.; Bradbury, J.

    1995-12-01

    Four issues commonly form the framework for debates about the acceptability of proposed projects or technologies--the substantive decision or technological choice; the treatment of the community by the proponent organization; the way the decision-making process has been structured and managed; and the status of institutional safeguards and protection. One of the clear messages of cultural theory is that differences in perspectives are a normal and inevitable part of society, and that attempts to resolve differences by persuasion are not likely to work. These findings are useful when considering the goals and possibilities of public involvement as a decision-making tool, and when designing or evaluating public involvement training programs for managers. The research reported here examines the viewpoints and concerns of managers and decision-makers about the four issues identified above, with particular emphasis on their perspectives and concerns about opening decision-making processes to the public and about managers` roles and responsibilities for structuring and managing open decision-making processes. Implications of these findings for public involvement training for managers is also discussed. The data presented in this paper were obtained from face-to-face interviews with managers and decision-makers with experience managing a variety of hazardous waste management decision-making processes. We conducted these interviews in the course of four separate research projects: needs assessments to support the design and development of a public involvement training program for managers; a study of community residents` and managers` perspectives on the chemical stockpile disposal program; an evaluation of the effectiveness of public involvement training for managers in the Department of Energy; and a study to develop indicators of the benefits and costs of public involvement.

  11. Public Involvement and Communications Committee Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    June 1, 2011 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT & COMMUNICATIONS COMMITTEE MEETING June 1, 2011 Kennewick, WA Topics in this Meeting Summary Welcome and Introductions .......................................................................................................................... 1 Comprehensive Debrief - State of the Site Meetings ................................................................................... 4 Public Meetings - PW-1/3/6 and CW-5

  12. Sandia National Laboratories: Community Involvement: Volunteer Programs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Community Involvement Contribution Programs Volunteer Programs Education Programs About Volunteer Programs Helping where we are needed Habitat for Humanity Employees and retirees are passionate about volunteering. You'll find Sandians judging science fairs, coaching sports teams, leading scouting troops, sorting food, building houses, and serving on community boards. In support of individual volunteer efforts, Sandia provides a volunteer website to advertise current volunteer opportunities and

  13. Sandia National Laboratories: About Sandia: Community Involvement:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Education Programs: Science, Technology, Engineering and Math Programs Science, Technology, Engineering, and Math Programs Future scientists photo Our science, technology, engineering, and math (STEM) programs engage students by linking science to the real world. We offer three unique programs for American Indian students, African American students, and Hispanic students. The programs get students involved in fun, hands-on science and engineering activities and provide an opportunity to

  14. Radiative Decays Involving Light Scalar Mesons

    SciTech Connect (OSTI)

    Deirdre Black; Masayasu Harada; Joseph Schechter

    2002-09-01

    We present a vector dominance model to describe radiative decays involving the light scalar mesons. Assuming that the light scalar mesons form a nonet this model gives relations among various decay amplitudes of the form S--> gamma gamma, V-->Sgamma and S--> Vgamma, where S and V denote scalar and vector mesons. By comparing with experimental radiative decay rates, including those recently measured for phi--> PP'gamma, we obtain various predictions. We discuss briefly ongoing extensions of the present work in an attempt to describe the radiative phi decay measurements in detail.

  15. Apolipoprotein gene involved in lipid metabolism

    DOE Patents [OSTI]

    Rubin, Edward; Pennacchio, Len A.

    2007-07-03

    Methods and materials for studying the effects of a newly identified human gene, APOAV, and the corresponding mouse gene apoAV. The sequences of the genes are given, and transgenic animals which either contain the gene or have the endogenous gene knocked out are described. In addition, single nucleotide polymorphisms (SNPs) in the gene are described and characterized. It is demonstrated that certain SNPs are associated with diseases involving lipids and triglycerides and other metabolic diseases. These SNPs may be used alone or with SNPs from other genes to study individual risk factors. Methods for intervention in lipid diseases, including the screening of drugs to treat lipid-related or diabetic diseases are also disclosed.

  16. Genomic Aspects of Research Involving Polyploid Plants

    SciTech Connect (OSTI)

    Yang, Xiaohan [ORNL; Ye, Chuyu [ORNL; Tschaplinski, Timothy J [ORNL; Wullschleger, Stan D [ORNL; Tuskan, Gerald A [ORNL

    2011-01-01

    Almost all extant plant species have spontaneously doubled their genomes at least once in their evolutionary histories, resulting in polyploidy which provided a rich genomic resource for evolutionary processes. Moreover, superior polyploid clones have been created during the process of crop domestication. Polyploid plants generated by evolutionary processes and/or crop domestication have been the intentional or serendipitous focus of research dealing with the dynamics and consequences of genome evolution. One of the new trends in genomics research is to create synthetic polyploid plants which provide materials for studying the initial genomic changes/responses immediately after polyploid formation. Polyploid plants are also used in functional genomics research to study gene expression in a complex genomic background. In this review, we summarize the recent progress in genomics research involving ancient, young, and synthetic polyploid plants, with a focus on genome size evolution, genomics diversity, genomic rearrangement, genetic and epigenetic changes in duplicated genes, gene discovery, and comparative genomics. Implications on plant sciences including evolution, functional genomics, and plant breeding are presented. It is anticipated that polyploids will be a regular subject of genomics research in the foreseeable future as the rapid advances in DNA sequencing technology create unprecedented opportunities for discovering and monitoring genomic and transcriptomic changes in polyploid plants. The fast accumulation of knowledge on polyploid formation, maintenance, and divergence at whole-genome and subgenome levels will not only help plant biologists understand how plants have evolved and diversified, but also assist plant breeders in designing new strategies for crop improvement.

  17. 10.0 COMMUNITY RELATIONS/PUBLIC INVOLVEMENT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0-1 10.0 COMMUNITY RELATIONSPUBLIC INVOLVEMENT 10.1 INTRODUCTION This section describes, in general, the way in which the public will be involved with the implementation of this...

  18. Workplace Charging Challenge Partner: JLA Public Involvement | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy JLA Public Involvement Workplace Charging Challenge Partner: JLA Public Involvement Workplace Charging Challenge Partner: JLA Public Involvement Joined the Challenge: March 2013 Headquarters: Portland, OR Charging Location: Portland, OR Domestic Employees: 15 Purchasing a plug-in electric vehicle (PEV) and installing a charging station has expanded JLA Public Involvement's sustainability efforts and allowed them to achieve Gold certification in the City of Portland's Sustainability

  19. Prototype indicators of value added through public involvement

    SciTech Connect (OSTI)

    Lach, D.; Hixson, P.; Ramonas, L.

    1995-12-01

    As more managers realize that public input in public sector decision making is a given in the current political and social climate, many are turning to public involvement as a way to manage input so that it is beneficial to their decisions and projects. Public involvement is starting to become a familiar way of doing business for many Federal agencies and its contractors. Yet, many, if not most agency and contractor managers are still unclear about the value and costs that public involvement adds to their projects. Proponents claim that public involvement increases the acceptability of project goals by increasing stakeholders` knowledge about and involvement in decisions of importance to them. In spite of these assertions avowing the benefits of public involvement, proponents have not generated methods that demonstrate or provide evidence of such value added through incorporating public involvement into projects. As these questions about the value and costs of public involvement efforts increase, there is a pressing need to document the value and costs of public involvement for the participants in these processes--the stakeholders--and to present this information to decision makers in ways that help them assess the value and costs of managing public input through a public involvement program.

  20. DOE Involvement in Consensus Negotiations for Energy Conservation Standards

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Involvement in Consensus Negotiations for Energy Conservation Standards DOE Involvement in Consensus Negotiations for Energy Conservation Standards DOE Involvement in Consensus Negotiations for Energy Conservation Standards. The Department of Energy is supportive of outside parties' negotiations efforts to reach consensus on energy conservation standards and test procedures that can be presented for consideration by the Department However, neither the Department nor

  1. Draft Public Involvement Advice for Central Plateau Cleanup

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    V0, 12914 Draft Public Involvement Advice for Central Plateau Cleanup Principles Issue managers: Pollet, Mattson, Vanni, Plahuta, Catrell Background The Tri-Party Agencies are...

  2. Tri-Party Agreement Hanford Public Involvement Plan

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    from the Comment and Response Document on the Tri-Party Agreement Hanford Public Involvement Plan The comments submitted by the Hanford Advisory Board (Advice 251) were...

  3. Tri-Party Agreement Agencies - Public involvement calendar -...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TRI-PARTY AGREEMENT AGENCIES - PUBLIC INVOLVEMENT CALENDAR - FISCAL YEAR 2015 - SEPTEMBER 2015 EVENTS IN PROGRESSCOMING SOON September 2015 October 2015 November 2015 Tri-Party...

  4. FOIASI - Special Inquiry Review of Allegations InvolvingPotentialMisc...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The allegations involved potential violations of political activity restrictions, lack of impartiality in performing official duties, misuse of position, and other related ...

  5. Multidomain Carbohydrate-binding Proteins Involved in Bacteroides

    Office of Scientific and Technical Information (OSTI)

    thetaiotaomicron Starch Metabolism (Journal Article) | SciTech Connect Multidomain Carbohydrate-binding Proteins Involved in Bacteroides thetaiotaomicron Starch Metabolism Citation Details In-Document Search Title: Multidomain Carbohydrate-binding Proteins Involved in Bacteroides thetaiotaomicron Starch Metabolism Authors: Cameron, Elizabeth A. ; Maynard, Mallory A. ; Smith, Christopher J. ; Smith, Thomas J. ; Koropatkin, Nicole M. ; Martens, Eric C. [1] ; Danforth) [2] + Show Author

  6. Best Practices for Emergency Response to Incidents Involving...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    -- Page iii -- FOREWORD Fires involving cars, trucks and other highway vehicles are a ... and PPE 194 8.2.1 General Procedures for Hybrid and EDV Fire Suppression 194 8.2.2 ...

  7. Getting Involved in Manufacturing Day – College Educators Edition Webinar

    Broader source: Energy.gov [DOE]

    This webinar will help address questions on why and how community colleges, universities, and technical schools can get involved with MFG DAY.  Led by MFG DAY Co-Producers, the webinar will also...

  8. Drum Involved in Idaho Incident Not Shippable to WIPP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The drum involved in the venting incident was not shippable to WIPP as packaged. Sealed drums cannot be shipped to WIPP without venting. The drum in the incident had been sealed...

  9. Global prevalence and distribution of genes and microorganisms involved in

    Office of Scientific and Technical Information (OSTI)

    mercury methylation (Journal Article) | DOE PAGES Global prevalence and distribution of genes and microorganisms involved in mercury methylation « Prev Next » Title: Global prevalence and distribution of genes and microorganisms involved in mercury methylation Mercury methylation produces the neurotoxic, highly bioaccumulative methylmercury (MeHg). Recent identification of the methylation genes (hgcAB) provides the foundation for broadly evaluating microbial Hg-methylation potential in

  10. Integrated Analysis of Protein Complexes and Regulatory Networks Involved

    Office of Scientific and Technical Information (OSTI)

    in Anaerobic Energy Metabolism of Shewanella Oneidensis MR-1 (Technical Report) | SciTech Connect Technical Report: Integrated Analysis of Protein Complexes and Regulatory Networks Involved in Anaerobic Energy Metabolism of Shewanella Oneidensis MR-1 Citation Details In-Document Search Title: Integrated Analysis of Protein Complexes and Regulatory Networks Involved in Anaerobic Energy Metabolism of Shewanella Oneidensis MR-1 Anaerobic Nitrate Reduction. Nitrate is an extensive co-contaminant

  11. Memory and Spin Injection Devices Involving Half Metals (Journal Article) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Memory and Spin Injection Devices Involving Half Metals Citation Details In-Document Search Title: Memory and Spin Injection Devices Involving Half Metals Authors: Shaughnessy, M. ; Snow, Ryan ; Damewood, L. ; Fong, C. Y. Publication Date: 2011-01-01 OSTI Identifier: 1198038 Grant/Contract Number: AC52-07NA27344 Type: Published Article Journal Name: Journal of Nanomaterials Additional Journal Information: Journal Volume: 2011; Journal ID: ISSN 1687-4110 Publisher: Hindawi

  12. NEPA-Related Public Involvement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    NEPA-Related Public Involvement NEPA-Related Public Involvement The Loan Programs Office's NEPA-related hearings, public meetings, and public notices (e.g. public scoping meeting, public hearing, notice of proposed floodplain or wetland action) are presented below under NEPA-Related Hearings, Meetings, and Notices. Available environmental documents can be found at the following links: Categorical Exclusions Environmental Assessments Environmental Impact Statements NEPA-Related Hearings,

  13. Accurate Numerical Simulations Of Chemical Phenomena Involved in Energy

    Office of Scientific and Technical Information (OSTI)

    Production and Storage with MADNESS and MPQC: ALCF-2 Early Science Program Technical Report (Technical Report) | SciTech Connect Technical Report: Accurate Numerical Simulations Of Chemical Phenomena Involved in Energy Production and Storage with MADNESS and MPQC: ALCF-2 Early Science Program Technical Report Citation Details In-Document Search Title: Accurate Numerical Simulations Of Chemical Phenomena Involved in Energy Production and Storage with MADNESS and MPQC: ALCF-2 Early Science

  14. Get Involved with Home Energy Score | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Home Energy Score » Get Involved with Home Energy Score Get Involved with Home Energy Score The Department of Energy's (DOE) Home Energy Score helps homeowners understand the energy performance of their homes and how they compare to other homes nationwide. Energy professionals and organizations can use the Score to promote energy efficiency to homeowners and encourage investments in energy improvements. Information for Homeowners Information for Interested Organizations Information for

  15. Metaproteomics Identifies the Protein Machinery Involved in Metal and

    Office of Scientific and Technical Information (OSTI)

    Radionuclide Reduction in Subsurface Microbiomes and Elucidates Mechanisms and U(VI) Reduction Immobilization (Technical Report) | SciTech Connect Technical Report: Metaproteomics Identifies the Protein Machinery Involved in Metal and Radionuclide Reduction in Subsurface Microbiomes and Elucidates Mechanisms and U(VI) Reduction Immobilization Citation Details In-Document Search Title: Metaproteomics Identifies the Protein Machinery Involved in Metal and Radionuclide Reduction in Subsurface

  16. Consistent analysis of one-nucleon spectroscopic factors involving weakly-

    Office of Scientific and Technical Information (OSTI)

    and strongly-bound nucleons (Journal Article) | SciTech Connect Consistent analysis of one-nucleon spectroscopic factors involving weakly- and strongly-bound nucleons Citation Details In-Document Search Title: Consistent analysis of one-nucleon spectroscopic factors involving weakly- and strongly-bound nucleons Authors: Okołowicz, J. ; Lam, Y. H. ; Płoszajczak, M. ; Macchiavelli, A. O. ; Smirnova, N. A. Publication Date: 2016-04-01 OSTI Identifier: 1246192 Grant/Contract Number:

  17. Emergency Response to a Transportation Accident Involving Radioactive

    Office of Environmental Management (EM)

    Material | Department of Energy Response to a Transportation Accident Involving Radioactive Material Emergency Response to a Transportation Accident Involving Radioactive Material The purpose of this User's Guide is to provide instructors with an overview of the key points covered in the video. The Student Handout portion of this Guide is designed to assist the instructor in reviewing those points with students. The Student Handout should be distributed to students after the video is shown

  18. Draft Public Involvement Advice for Central Plateau Cleanup

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    V0, 12/9/14 Draft Public Involvement Advice for Central Plateau Cleanup Principles Issue managers: Pollet, Mattson, Vanni, Plahuta, Catrell Background The Tri-Party Agencies are developing principles that will guide the cleanup of Hanford's Central Plateau. The Hanford Advisory Board (HAB) believes that the broader public should be involved in the development of these principles. Hanford's Central Plateau is planned to be the final footprint of the Hanford Site. The Central Plateau contains

  19. Community assessments: Road maps to successful public involvement

    SciTech Connect (OSTI)

    Forrest, C.J.

    1995-12-01

    This paper presents a framework for performing comprehensive community assessments to increase the effectiveness of public involvement efforts. The community assessment process involves systematic and critical examination of beliefs, attitudes, opinions and knowledge of the many groups in the community. A good community assessment gathers information on the concerns or issues directly related to an environmental project as well as other important community issues to ensure development of an effective public involvement program. The framework discusses types of people and organizations that should be included in the community assessment interview process. It also delineates steps that persons charged with performing assessments can take to ensure that the views of all potential stakeholders are included--a necessary consideration in communities that are ethnically, racially, or economically diverse. Lastly, the framework discusses types of written materials that can be analyzed for additional insight into communities. For example, it discusses methods for reviewing past issues of the local newspaper to provide both validation of the information gathered during interviews and an understanding of the ways that community members give and receive information and respond to conflict or perceived threats. This framework is designed to show environmental professionals who are responsible for public involvement how to perform an initial community assessment. It provides illustrations of instances in which information vital to a successful public involvement effort has been uncovered through a careful assessment of the community. Both the U.S. EPA and the public have made clear their intentions to require increased public involvement. Many of these efforts will succeed or fail based on how well those charged with the public involvement component of environmental projects identify community issues and design communications programs that successfully reach all stakeholders.

  20. GC GUIDANCE ON BARTER TRANSACTIONS INVOLVING DOE-OWNED URANIUM

    Energy Savers [EERE]

    GC GUIDANCE ON BARTER TRANSACTIONS INVOLVING DOE-OWNED URANIUM The Department of Energy has on a variety of occasions engaged in transactions under which it bartered uranium to which it has title for goods or services . This guidance memorializes the results of analyses previously directed to individual proposed transactions . For the reasons discussed below, we conclude that the Atomic Energy Act of 1954' , as amended, (AEA), authorizes such barter transactions. Background : DOE Barter

  1. Tri-Party Agreement Agencies Annual Hanford Public Involvement Survey

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    7 Tri-Party Agreement Agencies Annual Hanford Public Involvement Survey 1. How do you usually receive information about Hanford topics? (Please select all that apply) Response Percent Response Count Advisory Board 23.7% 22 Citizens Group 32.3% 30 Ecology website 21.5% 20 Hanford.gov website 33.3% 31 Hanford Listserv (email) 54.8% 51 Hanford Mailing (postal) 12.9% 12 Newspaper 48.4% 45 Radio 26.9% 25 Social Media (Facebook, Twitter, etc.) 26.9% 25 Television 17.2% 16 Work Announcements 11.8% 11 I

  2. Probative Investigation of the Thermal Stability of Wastes Involved in

    Energy Savers [EERE]

    ProActive DNS Blacklisting ProActive DNS Blacklisting PDF icon Tuesday_Fair_Park_2_1330_Rackow.pdf More Documents & Publications DNS as a Covert Channel Within Protected Networks Cyber Security Procurement Language for Control Systems Version 1.8 Slide February 2014 Waste Isolation Pilot Plant (WIPP) Waste Drum Breach Event | Department of Energy

    Probative Investigation of the Thermal Stability of Wastes Involved in February 2014 Waste Isolation Pilot Plant (WIPP) Waste Drum Breach

  3. Central Plateau Principles Public Involvement Advice DETAILED BACKGROUND

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    v0, 12/9/14 Central Plateau Principles Public Involvement Advice DETAILED BACKGROUND Cleanup of Hanford's Central Plateau is expected to take another four decades or longer, and cost tens of billions of dollars. The Central Plateau includes the 200 East and 200 West Areas with all of Hanford's High-Level Nuclear Waste Tank Farms, processing plants, sites where over a million gallons of High-Level Nuclear Waste has leaked from Single Shell Tanks (SSTs), and billions of gallons of waste was

  4. Antitrust aspects of involvement by utilities in residential solar energy

    SciTech Connect (OSTI)

    Hurst, T.R.

    1983-01-01

    A utility that merely engages in the sale of solar units produced by independent manufacturers on a nonexclusive basis appears to run little risk of violating federal antitrust laws, but virtually every other unregulated activity would involve enough significant exposure to antitrust liability to preclude a prudent utility from engaging in them on any basis other than one where the state-action exemption clearly shields the utility. The case cited in this review indicates that municipal utilities must be equally cautious when engaging in pro-solar activities that may have an anticompetitive impact on various parties who could become plaintiffs in a private antitrust action. 47 references.

  5. Market power and foreign involvement by US multinationals

    SciTech Connect (OSTI)

    Hirschey, M.

    1982-05-01

    This study considers the relationship between market power and multinational involvement through use of a market-valuation approach. Estimation results for a sample of large US multinationals reveal superior valuation effects due to returns from foreign as opposed to domestic operations. This finding is consistent with the hypothesis that returns from the US market tend to be less secure, and therefore less valued, than are returns from foreign markets due to both real (market size, entry barriers, etc.) and institutional (antitrust policies, etc.) differences in competitive environments. Such findings are also consistent with previous suggestions that firms develop markets abroad in order to exploit economic-rent opportunities. These findings remain tentative, however, and await verification in future studies of data from both the United States and abroad. 13 references, 1 table.

  6. Representation of analysis results involving aleatory and epistemic uncertainty.

    SciTech Connect (OSTI)

    Johnson, Jay Dean; Helton, Jon Craig; Oberkampf, William Louis; Sallaberry, Cedric J.

    2008-08-01

    Procedures are described for the representation of results in analyses that involve both aleatory uncertainty and epistemic uncertainty, with aleatory uncertainty deriving from an inherent randomness in the behavior of the system under study and epistemic uncertainty deriving from a lack of knowledge about the appropriate values to use for quantities that are assumed to have fixed but poorly known values in the context of a specific study. Aleatory uncertainty is usually represented with probability and leads to cumulative distribution functions (CDFs) or complementary cumulative distribution functions (CCDFs) for analysis results of interest. Several mathematical structures are available for the representation of epistemic uncertainty, including interval analysis, possibility theory, evidence theory and probability theory. In the presence of epistemic uncertainty, there is not a single CDF or CCDF for a given analysis result. Rather, there is a family of CDFs and a corresponding family of CCDFs that derive from epistemic uncertainty and have an uncertainty structure that derives from the particular uncertainty structure (i.e., interval analysis, possibility theory, evidence theory, probability theory) used to represent epistemic uncertainty. Graphical formats for the representation of epistemic uncertainty in families of CDFs and CCDFs are investigated and presented for the indicated characterizations of epistemic uncertainty.

  7. Personnel involved in nuclear standards development: 1980 directory

    SciTech Connect (OSTI)

    Carr, S.J.

    1980-03-01

    The development of nuclear standards is an active and necessary endeavor that is concerned with the safe, orderly, and economic development of nuclear potential. There are almost 4100 people from the teachnical community who are presently involved either in writing nuclear standards, including codes, or in the management and processing roles necessary for their approval and promulgation. This document identifies the current participation of each individual as member, chairman, cochairman (vice-chairman), or secretary of about 700 standards development committees and groups. The standards committees and groups are identified with the organizations that are responsible for the preparation, review, and maintenance of the standards and that provide support through supervisory committees and headquarters staff. This directory includes four major sections: personnel, employers, committees, and a Key-Word-in-Context (KWIC) Index of committee titles. It can also be used to identify the participation of employers as well as to recognize the contributions of individuals to the often interdisciplinary activity of standards development.

  8. Surface interactions involved in flashover with high density electronegative gases.

    SciTech Connect (OSTI)

    Hodge, Keith Conquest; Warne, Larry Kevin; Jorgenson, Roy Eberhardt; Wallace, Zachariah Red; Lehr, Jane Marie

    2010-01-01

    This report examines the interactions involved with flashover along a surface in high density electronegative gases. The focus is on fast ionization processes rather than the later time ionic drift or thermalization of the discharge. A kinetic simulation of the gas and surface is used to examine electron multiplication and includes gas collision, excitation and ionization, and attachment processes, gas photoionization and surface photoemission processes, as well as surface attachment. These rates are then used in a 1.5D fluid ionization wave (streamer) model to study streamer propagation with and without the surface in air and in SF6. The 1.5D model therefore includes rates for all these processes. To get a better estimate for the behavior of the radius we have studied radial expansion of the streamer in air and in SF6. The focus of the modeling is on voltage and field level changes (with and without a surface) rather than secondary effects, such as, velocities or changes in discharge path. An experiment has been set up to carry out measurements of threshold voltages, streamer velocities, and other discharge characteristics. This setup includes both electrical and photographic diagnostics (streak and framing cameras). We have observed little change in critical field levels (where avalanche multiplication sets in) in the gas alone versus with the surface. Comparisons between model calculations and experimental measurements are in agreement with this. We have examined streamer sustaining fields (field which maintains ionization wave propagation) in the gas and on the surface. Agreement of the gas levels with available literature is good and agreement between experiment and calculation is good also. Model calculations do not indicate much difference between the gas alone versus the surface levels. Experiments have identified differences in velocity between streamers on the surface and in the gas alone (the surface values being larger).

  9. Classroom simulation of public involvement in H.L.W. issues featuring STS concepts

    SciTech Connect (OSTI)

    Bieniawski, Z.T.

    1996-12-01

    This paper reports on a classroom experiment, now in its third year, conducted by the author for students involvement in High-Level Waste disposal. The project involved designing a methodology for HLW management in the United States.

  10. R-Axion: A New LHC Physics Signature Involving Muon Pairs (Conference...

    Office of Scientific and Technical Information (OSTI)

    Conference: R-Axion: A New LHC Physics Signature Involving Muon Pairs Citation Details In-Document Search Title: R-Axion: A New LHC Physics Signature Involving Muon Pairs In a class ...

  11. Getting Involved

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    received help navigating the draft EIS and asked questions about the project, the EIS process, documentation and how to submit comments on the document. There were no formal...

  12. Customer Involvement

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Projects Expand Projects Skip navigation links Ancillary and Control Area Services (ACS) Practices Forum Meetings Customer Comments Attachment K 2015 Planning Cycle 2014...

  13. Involvement & Outreach

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Volunteers - Sign Up About Science Bowl Curriculum and Activities How to Build a Motor The Great Marble Drop How to Build a Turbine How to Build a Tower Classroom...

  14. 01-12-1998 - Bench Top FIre Involving Use of Alcohol and Burner...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1998 - Bench Top FIre Involving Use of Alcohol and Burner Document Number: NA Effective Date: 011998 File (public): PDF icon 01-12-1998...

  15. Public involvement in the regulatory activities regarding nuclear fuel cycle facilities: A case study

    SciTech Connect (OSTI)

    Austin, D.E.

    1995-12-01

    This paper reviews the involvement of a community-based organization in the activities of the Nuclear Regulatory Commission (NRC) regarding a uranium conversion facility that operated near Gore, Oklahoma from 1970 until 1992. Effective participation requires access to decision making. Access is a complex phenomenon that includes: (1) opportunity, both procedural and physical, (2) a common language, and (3) time and resources. The paper describes how both the community organization and the NRC responded to these requirements for access, the strategies that were most effective in securing meaningful public involvement in the decision making, and the impacts of that involvement on the organization.

  16. LANL gets young women involved in math and science at 31st Expanding...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LANL gets young women involved in math and science at 31st Expanding Your Horizons ... the Expanding Your Horizons in Math and Science(tm) Conference to give middle- ...

  17. Molecular genetic analysis of activation-tagged transcription factors thought to be involved in photomorphogenesis

    SciTech Connect (OSTI)

    Neff, Michael M.

    2011-06-23

    This is a final report for Department of Energy Grant No. DE-FG02-08ER15927 entitled “Molecular Genetic Analysis of Activation-Tagged Transcription Factors Thought to be Involved in Photomorphogenesis”. Based on our preliminary photobiological and genetic analysis of the sob1-D mutant, we hypothesized that OBP3 is a transcription factor involved in both phytochrome and cryptochrome-mediated signal transduction. In addition, we hypothesized that OBP3 is involved in auxin signaling and root development. Based on our preliminary photobiological and genetic analysis of the sob2-D mutant, we also hypothesized that a related gene, LEP, is involved in hormone signaling and seedling development.

  18. 01-12-1998 - Bench Top FIre Involving Use of Alcohol and Burner | The Ames

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory 1998 - Bench Top FIre Involving Use of Alcohol and Burner Document Number: NA Effective Date: 01/1998 File (public): PDF icon 01-12-1998

  19. Involvement of the [beta]3-[alpha]3 Loop of the Proline Dehydrogenase

    Office of Scientific and Technical Information (OSTI)

    Domain in Allosteric Regulation of Membrane Association of Proline Utilization A (Journal Article) | SciTech Connect Involvement of the [beta]3-[alpha]3 Loop of the Proline Dehydrogenase Domain in Allosteric Regulation of Membrane Association of Proline Utilization A Citation Details In-Document Search Title: Involvement of the [beta]3-[alpha]3 Loop of the Proline Dehydrogenase Domain in Allosteric Regulation of Membrane Association of Proline Utilization A Authors: Zhu, Weidong ; Haile,

  20. Structures of the PelD Cyclic Diguanylate Effector Involved in Pellicle

    Office of Scientific and Technical Information (OSTI)

    Formation in Pseudomonas aeruginosa PAO1 (Journal Article) | SciTech Connect SciTech Connect Search Results Journal Article: Structures of the PelD Cyclic Diguanylate Effector Involved in Pellicle Formation in Pseudomonas aeruginosa PAO1 Citation Details In-Document Search Title: Structures of the PelD Cyclic Diguanylate Effector Involved in Pellicle Formation in Pseudomonas aeruginosa PAO1 Authors: Li, Zhi ; Chen, Jui-Hui ; Hao, Yue ; Nair, Satish K. [1] + Show Author Affiliations UIUC

  1. Discovery of a new ATP-binding motif involved in peptidic azoline

    Office of Scientific and Technical Information (OSTI)

    biosynthesis (Journal Article) | SciTech Connect Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis Citation Details In-Document Search Title: Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis Authors: Dunbar, Kyle L. ; Chekan, Jonathan R. ; Cox, Courtney L. ; Burkhart, Brandon J. ; Nair, Satish K. ; Mitchell , Douglas A. [1] + Show Author Affiliations (UIUC) Publication Date: 2014-11-20 OSTI Identifier: 1163365 Resource Type: Journal

  2. Public Understanding of Cleanup Levels DRAFT FAQ Public Involvement Committee Meeting, Hanford Advisory Board

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DRAFT FAQ Public Involvement Committee Meeting, Hanford Advisory Board Wednesday, June 5, 2013 at the Red Lion Hanford House in Richland Cleanup Levels The better the public understands how cleanup levels are set to determine what the acceptable levels of residual contamination may be left at Hanford, the more effective and meaningful their input into cleanup decisions will be. We must stay informed in order to stay involved and provide meaningful input. Here are some frequently asked questions

  3. Public Understanding of Cleanup Levels Discussion Public Involvement Committee Meeting - Hanford Advisory Board

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Discussion Public Involvement Committee Meeting - Hanford Advisory Board Wednesday, October 31, 2012 - Red Lion Hanford House, Richland Page 1 of 4 Cleanup Levels One of the reasons we want public involvement on cleanup decisions is to gather input about what kind of cleanup end-state the public wants, how clean is clean, or put another way, how dirty is acceptable. Cleanup decisions are complicated. Cleanup levels necessary to be protective dictate which cleanup actions are viable to consider.

  4. SO2907, A Putative TonB-dependent Receptor, Is Involved in Dissimilatory

    Office of Scientific and Technical Information (OSTI)

    Iron Reduction by Shewanella oneidensis Strain MR-1 (Journal Article) | SciTech Connect Journal Article: SO2907, A Putative TonB-dependent Receptor, Is Involved in Dissimilatory Iron Reduction by Shewanella oneidensis Strain MR-1 Citation Details In-Document Search Title: SO2907, A Putative TonB-dependent Receptor, Is Involved in Dissimilatory Iron Reduction by Shewanella oneidensis Strain MR-1 Shewanella oneidensis strain MR-1 utilizes soluble and insoluble ferric ions as terminal electron

  5. Proposed Tenaska Washington II Generation Project : Final Environmental Impact Statement. Volume 2: Public Involvement.

    SciTech Connect (OSTI)

    United States. Bonneville Power Administration.

    1994-01-01

    In regard to the proposed Tenaska Washington II Generation Project, the goal of the Bonneville Power Administration`s (BPA) Environmental Impact Statement (EIS) public involvement process is to determine the issues to be examined and pertinent analyses to be conducted and to solicit comments on the content and quality of information presented in the Draft Environmental Impact Statement (DEIS). Comments and questions are solicited from the public and government agencies during the scoping process and during the comment period and public hearing on the DEIS, to find out what is of most concern to them. The end product of the public involvement process is the Comment Report which follows in part of this volume on Public Involvement.

  6. Getting the lead out: Citizen involvement in the Williamsburg Bridge lead paint removal project

    SciTech Connect (OSTI)

    Forker, T.R.

    1997-08-01

    This paper examines the process and results of citizen involvement in developing new environmental control and compliance procedures used by the New York City Department of Transportation (NYC DOT) for lead paint removal on the Williamsburg Bridge and other structures. As a case study of the effects of public involvement in environmental decision-making, the study identifies and discusses the factors that produced failures or successes in satisfying the citizen`s concerns about health risks and the effectiveness of the selected pollution control technology.

  7. Differing Professional Opinions Manual for Technical Issues Involving Environment, Safety, and Health

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2006-11-16

    This Manual establishes a differing professional opinion (DPO) policy to a Department of Energy (DOE) Differing Professional Opinion (DPO) Process to encourage and facilitate dialogue and resolution on DPOs from employees for technical issues involving environment, safety, and health. Does not cancel other directives. Canceled by DOE O 442.2.

  8. Final report on the public involvement process phase 1, Monitored Retrievable Storage Facility Feasibility Study

    SciTech Connect (OSTI)

    Moore, L.; Shanteau, C.

    1992-12-01

    This report summarizes the pubic involvement component of Phase 1 of the Monitored Retrievable Storage Facility (NM) Feasibility Study in San Juan County, Utah. Part of this summary includes background information on the federal effort to locate a voluntary site for temporary storage of nuclear waste, how San Juan County came to be involved, and a profile of the county. The heart of the report, however, summarizes the activities within the public involvement process, and the issues raised in those various forums. The authors have made every effort to reflect accurately and thoroughly all the concerns and suggestions expressed to us during the five month process. We hope that this report itself is a successful model of partnership with the citizens of the county -- the same kind of partnership the county is seeking to develop with its constituents. Finally, this report offers some suggestions to both county officials and residents alike. These suggestions concern how decision-making about the county's future can be done by a partnership of informed citizens and listening decision-makers. In the Appendix are materials relating to the public involvement process in San Juan County.

  9. Final report on the public involvement process phase 1, Monitored Retrievable Storage Facility Feasibility Study

    SciTech Connect (OSTI)

    Moore, L.; Shanteau, C.

    1992-12-01

    This report summarizes the pubic involvement component of Phase 1 of the Monitored Retrievable Storage Facility (NM) Feasibility Study in San Juan County, Utah. Part of this summary includes background information on the federal effort to locate a voluntary site for temporary storage of nuclear waste, how San Juan County came to be involved, and a profile of the county. The heart of the report, however, summarizes the activities within the public involvement process, and the issues raised in those various forums. The authors have made every effort to reflect accurately and thoroughly all the concerns and suggestions expressed to us during the five month process. We hope that this report itself is a successful model of partnership with the citizens of the county -- the same kind of partnership the county is seeking to develop with its constituents. Finally, this report offers some suggestions to both county officials and residents alike. These suggestions concern how decision-making about the county`s future can be done by a partnership of informed citizens and listening decision-makers. In the Appendix are materials relating to the public involvement process in San Juan County.

  10. The value of adding regional to local stakeholder involvement in evaluating the acceptability of innovative technologies

    SciTech Connect (OSTI)

    Peterson, T.; McCabe, G.; Niesen, K.; Patricia, S.

    1995-12-01

    Technology is urgently needed to clean up contamination by volatile organic compounds at United States Department of Energy (DOE) and industrial sites throughout the country. In many cases, however, existing technology is too slow, inefficient, or expensive. The record of technology development is similarly disappointing. Remediation technologies developed at great expense and evaluated piecemeal over long periods have not been deployed because, in the end, the public judged them ineffective or unacceptable. The need for remediation is too great and resources too limited to continue with ineffective technology evaluation. Early and continuing stakeholder involvement is essential to developing successful, deployable technologies. In order to make good decisions about which technologies to deploy, remedial project managers need to know stakeholders` requirements for the performance of proposed technologies. Expanding stakeholder involvement regionally identifies the concerns of a broad range of stakeholders at a arid DOE sites throughout the West - issues that must be taken into account if technologies are to be accepted for wide deployment.

  11. A statistical description of the types and severities of accidents involving tractor semi-trailers

    SciTech Connect (OSTI)

    Clauss, D.B.; Wilson, R.K.; Blower, D.F.; Campbell, K.L.

    1994-06-01

    This report provides a statistical description of the types and severities of tractor semi-trailer accidents involving at least one fatality. The data were developed for use in risk assessments of hazardous materials transportation. Several accident databases were reviewed to determine their suitability to the task. The TIFA (Trucks Involved in Fatal Accidents) database created at the University of Michigan Transportation Research Institute was extensively utilized. Supplementary data on collision and fire severity, which was not available in the TIFA database, were obtained by reviewing police reports for selected TIFA accidents. The results are described in terms of frequencies of different accident types and cumulative distribution functions for the peak contact velocity, rollover skid distance, fire temperature, fire size, fire separation, and fire duration.

  12. The people problems of NEPA: Social impact assessment and the role of public involvement

    SciTech Connect (OSTI)

    Carnes, S.A.

    1989-12-31

    This Chapter of the book `` The Scientific Challenges of NEPA`` discusses the people problems of NEPA and social impact assessment and the role of public involvement in NEPA. When Congress passed the National Environmental Policy Act (NEPA) in 1969, there was little guidance on the preparation of environmental impact statements (EIS) and the role of the public in the NEPA process. Excepting the statutory language of NEPA, which referred to impacts on the human environment, nowhere was this more evident than with respect to people. Questions such as what impacts on people should be assessed, how impacts on people should be assessed, and how people, including but not limited to those persons potentially impacted, should be involved in the assessment itself as well as NEPA`s associated administrative processes, were simply not addressed.

  13. The Department of Energy (DOE) is actively involved in leading the

    Energy Savers [EERE]

    (DOE) is actively involved in leading the development of advanced applications and tools to more effectively operate the electricity delivery system by enabling advanced analysis, visualization, monitoring and alarming, and decision support capabilities for grid operators. These applications use-and greatly increase the value of-data coming from high-resolution electrical measuring devices known as phasor measurement units (PMUs), whose deployment is being facilitated by the North American

  14. Temperature of aircraft cargo flame exposure during accidents involving fuel spills

    SciTech Connect (OSTI)

    Mansfield, J.A.

    1993-01-01

    This report describes an evaluation of flame exposure temperatures of weapons contained in alert (parked) bombers due to accidents that involve aircraft fuel fires. The evaluation includes two types of accident, collisions into an alert aircraft by an aircraft that is on landing or take-off, and engine start accidents. Both the B-1B and B-52 alert aircraft are included in the evaluation.

  15. Creating an Ethical Framework for Studies That Involve the Worker Community

    Office of Science (SC) Website

    | U.S. DOE Office of Science (SC) DOE Resources » Creating an Ethical Framework for Studies That Involve the Worker Community Human Subjects Protection Program (HSPP) HSPP Home About Institutional Review Boards (IRBs) Education and Resources CITI Courses DOE Resources Ethical Framework Informed Consent Special Research Categories Community IRB Member Resources Other Resources Human Subjects Research Database (HSRD) IRB7 Regulations and Requirements Glossary Abbreviations Contact BER Home

  16. Quality control procedures for dynamic treatment delivery techniques involving couch motion

    SciTech Connect (OSTI)

    Yu, Victoria Y.; Fahimian, Benjamin P.; Xing, Lei; Hristov, Dimitre H.

    2014-08-15

    In this study, the authors introduce and demonstrate quality control procedures for evaluating the geometric and dosimetric fidelity of dynamic treatment delivery techniques involving treatment couch motion synchronous with gantry and multileaf collimator (MLC). Tests were designed to evaluate positional accuracy, velocity constancy and accuracy for dynamic couch motion under a realistic weight load. A test evaluating the geometric accuracy of the system in delivering treatments over complex dynamic trajectories was also devised. Custom XML scripts that control the Varian TrueBeam STx (Serial #3) axes in Developer Mode were written to implement the delivery sequences for the tests. Delivered dose patterns were captured with radiographic film or the electronic portal imaging device. The couch translational accuracy in dynamic treatment mode was 0.01 cm. Rotational accuracy was within 0.3, with 0.04 cm displacement of the rotational axis. Dose intensity profiles capturing the velocity constancy and accuracy for translations and rotation exhibited standard deviation and maximum deviations below 3%. For complex delivery involving MLC and couch motions, the overall translational accuracy for reproducing programmed patterns was within 0.06 cm. The authors conclude that in Developer Mode, TrueBeam is capable of delivering dynamic treatment delivery techniques involving couch motion with good geometric and dosimetric fidelity.

  17. ABSTRACT: The Community Environmental Monitoring Program: Reducing Public Perception of Risk Through Stakeholder Involvement

    SciTech Connect (OSTI)

    T. Hartwell

    2007-02-28

    Between 1951 and 1992, 928 nuclear tests were conducted at the Nevada Test Site (NTS), including 100 atmospheric and 828 underground tests. Initial public reaction to the tests was largely supportive, but by the late 1950s this began to change, largely as a result of fear of the potential for adverse health effects to be caused by exposure to ionizing radiation resulting from the tests. The nuclear power plant accident at Three Mile Island in 1979 served to heighten these fears, as well as foster a general distrust of the federal agencies involved and low public confidence in monitoring results. Modeled after a similar program that involved the public in monitoring activities around the Three Mile Island nuclear power plant, the Community Environmental Monitoring Program (CEMP) has promoted stakeholder involvement, awareness, and understanding of radiological surveillance in communities surrounding the NTS since 1981. It involves stakeholders in the operation, data collection, and dissemination of information obtained from a network of 29 stations across a wide area of Nevada, Utah, and California. It is sponsored by the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (NNSA/NSO) and administered by the Desert Research Institute (DRI) of the Nevada System of Higher Education. Since assuming administration of the program in 2000, DRI has accomplished significant enhancements to the network's data collection and transmission capabilities. A robust datalogging and communications system allows for the near real-time transmission of data to a platform maintained by DRI's Western Regional Climate Center, where the data are uploaded and displayed on a publicly accessible web site (http://cemp.dri.edu/). Additionally, the CEMP can serve as part of an emergency response network in the event of an unplanned radiological release from the NTS, and also provides an excellent platform for testing new environmental sensor technologies. Finally, the CEMP provides training workshops for involved stakeholders, and educational programs, which help to alleviate public perception of risk of health effects from past activities conducted at the NTS.

  18. Identification and Characterization of Soluble Factors Involved in Delayed Effects of Low Dose Radiation. Final report

    SciTech Connect (OSTI)

    Baulch, Janet

    2013-09-11

    This is a 'glue grant' that was part of a DOE Low Dose project entitled 'Identification and Characterization of Soluble Factors Involved in Delayed Effects of Low Dose Radiation'. This collaborative program has involved Drs. David L. Springer from Pacific Northwest National Laboratory (PNNL), John H. Miller from Washington State University, Tri-cities (WSU) and William F. Morgan then from the University of Maryland, Baltimore (UMB). In July 2008, Dr. Morgan moved to PNNL and Dr. Janet E. Baulch became PI for this project at University of Maryland. In November of 2008, a one year extension with no new funds was requested to complete the proteomic analyses. The project stemmed from studies in the Morgan laboratory demonstrating that genomically unstable cells secret a soluble factor or factors into the culture medium, that cause cytogenetic aberrations and apoptosis in normal parental GM10115 cells. The purpose of this project was to identify the death inducing effect (DIE) factor or factors, estimate their relative abundance, identify the cell signaling pathways involved and finally recapitulate DIE in normal cells by exogenous manipulation of putative DIE factors in culture medium. As reported in detail in the previous progress report, analysis of culture medium from the parental cell line, and stable and unstable clones demonstrated inconsistent proteomic profiles as relate to candidate DIE factors. While the proposed proteomic analyses did not provide information that would allow DIE factors to be identified, the analyses provided another important set of observations. Proteomic analysis suggested that proteins associated with the cellular response to oxidative stress and mitochondrial function were elevated in the medium from unstable clones in a manner consistent with mitochondrial dysfunction. These findings correlate with previous studies of these clones that demonstrated functional differences between the mitochondria of stable and unstable clones. These mitochondrial abnormalities in the unstable clones contributes to oxidative stress.

  19. Electric-field enhanced performance in catalysis and solid-state devices involving gases

    DOE Patents [OSTI]

    Blackburn, Bryan M.; Wachsman, Eric D.; Van Assche, IV, Frederick Martin

    2015-05-19

    Electrode configurations for electric-field enhanced performance in catalysis and solid-state devices involving gases are provided. According to an embodiment, electric-field electrodes can be incorporated in devices such as gas sensors and fuel cells to shape an electric field provided with respect to sensing electrodes for the gas sensors and surfaces of the fuel cells. The shaped electric fields can alter surface dynamics, system thermodynamics, reaction kinetics, and adsorption/desorption processes. In one embodiment, ring-shaped electric-field electrodes can be provided around sensing electrodes of a planar gas sensor.

  20. On reaction mechanisms involved in the deuteron–induced surrogate reactions

    SciTech Connect (OSTI)

    Avrigeanu, M.; Avrigeanu, V.; Mănăilescu, C.

    2015-02-24

    An extended analysis of the nuclear reaction mechanisms involved within deuteron interaction with nuclei, namely the breakup, stripping, pick-up, pre-equilibrium emission, and evaporation from fully equilibrated compound nucleus, is presented in order to highlight the importance of the direct mechanisms still neglected in the analysis of deuteron-induced surrogate reactions. Particularly, the dominance of the breakup mechanism at low energies around the Coulomb barrier should be considered in the case of (d,x) surrogate reactions on actinide target nuclei.

  1. Fatal accidents involving roof falls in coal mining, 1996--1998

    SciTech Connect (OSTI)

    Not Available

    1999-01-01

    This publication presents information on fatalities involving roof and rib falls that occurred in coal mining operations from January 1996 through December 1998. It includes statistics for the fatalities, as well as abstracts, best practices and illustrations. Conclusion statements have been substituted for best practices where no Title 30 Code of Regulations violations were cited during the accident investigation. From January 1996 through December 1998, 36 miners died at coal operations from accidents classified as roof falls. The information in the report is based on statistics taken from the 1996 through 1998 MSHA Fatal Illustration Programs: Roof Fall Fatalities by District.

  2. Fatal accidents involving roof falls in coal mining, 1996--1998

    SciTech Connect (OSTI)

    1999-11-01

    This publication presents information on fatalities involving roof and rib falls that occurred in coal mining operations from January 1996 through December 1998. It includes statistics for the fatalities, as well as abstracts, best practices and illustrations. Conclusion statements have been substituted for best practices where no Title 30 Code of Regulations violations were cited during the accident investigation. From January 1996 through December 1998, 36 miners died at coal operations from accidents classified as roof falls. The information in the report is based on statistics taken from the 1996 through 1998 MSHA Fatal Illustration Programs: Roof Fall Fatalities by District.

  3. EIS-0467: Notice of Intent to Prepare an Environmental Impact Statement and Notice of Floodplains and Wetlands Involvement

    Broader source: Energy.gov [DOE]

    Acquisition of a Natural Gas Pipeline and Natural Gas Utility Service at the Hanford Site, Richland, WA, and Notice of Floodplains and Wetlands Involvement

  4. Pseudobond parameters for QM/MM studies involving nucleosides, nucleotides, and their analogs

    SciTech Connect (OSTI)

    Chaudret, Robin; Parks, Jerry M; Yang, Weitao

    2013-01-01

    In biological systems involving nucleosides, nucleotides, or their respective analogs, the ribose sugar moiety is the most common reaction site, for example, during DNA replication and repair. How- ever, nucleic bases, which comprise a sizable portion of nucleotide molecules, are usually unreactive during such processes. In quantum mechanical/molecular simulations of nucleic acid reactivity, it may therefore be advantageous to describe specific ribosyl or ribosyl phosphate groups quantum me- chanically and their respective nucleic bases with a molecular mechanics potential function. Here, we have extended the pseudobond approach to enable quantum mechanical/molecular mechanical simulations involving nucleotides, nucleosides, and their analogs in which the interface between the two subsystems is located between the sugar and the base, namely, the C(sp3) N(sp2) bond. The pseudobond parameters were optimized on a training set of 10 molecules representing several nu- cleotide and nucleoside bases and analogs, and they were then tested on a larger test set of 20 diverse molecules. Particular emphasis was placed on providing accurate geometries and electrostatic prop- erties, including electrostatic potential, natural bond orbital (NBO) and atoms in molecules (AIM) charges and AIM first moments. We also tested the optimized parameters on five nucleotide and nu- cleoside analogues of pharmaceutical relevance and a small polypeptide (triglycine). Accuracy was maintained for these systems, which highlights the generality and transferability of the pseudobond approach. 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4772182

  5. Examining the consistency relations describing the three-point functions involving tensors

    SciTech Connect (OSTI)

    Sreenath, V.; Sriramkumar, L. E-mail: sriram@physics.iitm.ac.in

    2014-10-01

    It is well known that the non-Gaussianity parameter f{sub NL} characterizing the scalar bi-spectrum can be expressed in terms of the scalar spectral index in the squeezed limit, a property that is referred to as the consistency relation. In contrast to the scalar bi-spectrum, the three-point cross-correlations involving scalars and tensors and the tensor bi-spectrum have not received adequate attention, which can be largely attributed to the fact that the tensors had remained undetected at the level of the power spectrum until very recently. The detection of the imprints of the primordial tensor perturbations by BICEP2 and its indication of a rather high tensor-to-scalar ratio, if confirmed, can open up a new window for understanding the tensor perturbations, not only at the level of the power spectrum, but also in the realm of non-Gaussianities. In this work, we consider the consistency relations associated with the three-point cross-correlations involving scalars and tensors as well as the tensor bi-spectrum in inflationary models driven by a single, canonical, scalar field. Characterizing the cross-correlations in terms of the dimensionless non-Gaussianity parameters C{sub NL}{sup R} and C{sub NL}{sup γ} that we had introduced earlier, we express the consistency relations governing the cross-correlations as relations between these non-Gaussianity parameters and the scalar or tensor spectral indices, in a fashion similar to that of the purely scalar case. We also discuss the corresponding relation for the non-Gaussianity parameter h{sub NL} used to describe the tensor bi-spectrum. We analytically establish these consistency relations explicitly in the following two situations: a simple example involving a specific case of power law inflation and a non-trivial scenario in the so-called Starobinsky model that is governed by a linear potential with a sharp change in its slope. We also numerically verify the consistency relations in three types of inflationary models that permit deviations from slow roll and lead to scalar power spectra with features which typically result in an improved fit to the data than the more conventional, nearly scale invariant, spectra. We close with a summary of the results we have obtained.

  6. Pore scale modeling of reactive transport involved in geologic CO2 sequestration

    SciTech Connect (OSTI)

    Kang, Qinjin; Lichtner, Peter C; Viswanathan, Hari S; Abdel-fattah, Amr I

    2009-01-01

    We apply a multi-component reactive transport lattice Boltzmann model developed in previolls studies to modeling the injection of a C02 saturated brine into various porous media structures at temperature T=25 and 80 C. The porous media are originally consisted of calcite. A chemical system consisting of Na+, Ca2+, Mg2+, H+, CO2(aq), and CI-is considered. The fluid flow, advection and diHusion of aqueous species, homogeneous reactions occurring in the bulk fluid, as weB as the dissolution of calcite and precipitation of dolomite are simulated at the pore scale. The effects of porous media structure on reactive transport are investigated. The results are compared with continuum scale modeling and the agreement and discrepancy are discussed. This work may shed some light on the fundamental physics occurring at the pore scale for reactive transport involved in geologic C02 sequestration.

  7. Yellow affinity substance involved in the cellulolytic system of Clostridium thermocellum

    SciTech Connect (OSTI)

    Ljungdahl, L.G.; Pettersson, B.; Eriksson, K.E.; Wiegel, J.

    1983-01-01

    Clostridium thermocellum produces a yellow substance when fermenting cellulose. This substance is attached to the cellulose particles. Cellulose with the yellow substance, obtained from cultures of C. thermocellum, binds effectively endo-1,4-..beta..-glucanase produced by the bacterium and was used in an affinity column for purification of the enzyme. At the beginning of fermentation of cellulose, most of the endoglucanase was bound to the yellow cellulose. As the fermentation proceeded, the enzyme appeared free in the culture fluid. The endoglucanase bound to the yellow cellulose could be extracted by distilled water from the cellulose, but not by solutions with 5 mM or higher concentrations of salts or buffers. It is proposed that the yellow substance is involved in the cellulolytic system of C. thermocellum. 30 references, 3 figures, 3 tables.

  8. Success stories? an analysis of stakeholder involvement in the Department of Energy`s Office of Environmental Management

    SciTech Connect (OSTI)

    Beck, D.M.; Kelly, C.; Forbes, E.

    1995-12-01

    In several studies conducted by the Office of Environmental Management, the Department of Energy (DOE) has begun its first analysis of the benefits of stakeholder involvement. The studies seek to determine how public participation in DOE activities has contributed to cost savings, has changed the course of action for environmental projects, and has influenced the priorities of a national environmental program. This presentation will discuss the results and conclusions of following three studies: Stakeholder Survey of Trust and Confidence; the Success Stories study; and the Site-Specific Advisory Board Evaluation. These studies are taking their data from environmental management projects at Department of Energy sites throughout the United States. The studies of stakeholder involvement are currently analyzing the role of several variables in stakeholder involvement including the climate surrounding the environmental project or decision, the strategies used by DOE, the nature of the actual interactions, the attempts to reach consensus among interested parties, the role played by primary decision-makers, the goals set by the Department or by its stakeholders, and the revision of strategies during the stakeholder involvement process. Based on this analysis the planning and execution of future stakeholder involvement activities in Environmental Management will be improved. The study goal of establishing a set of factors that characterize successful stakeholder involvement within the Environmental Management program, will allow inference as to stakeholder involvement in other Federal and private environmental projects.

  9. Endovascular Treatment of Infrarenal Abdominal Aortic Lesions With or Without Common Iliac Artery Involvement

    SciTech Connect (OSTI)

    Oender, Hakan; Oguzkurt, Levent; Guer, Serkan; Tekbas, Gueven; Guerel, Kamil; Coskun, Isa; Oezkan, Ugur

    2013-02-15

    To evaluate the results of stent placement for obstructive atherosclerotic aortic disease with or without involvement of the common iliac artery. Forty patients had self-expanding stents primarily or after balloon dilatation in the abdominal aorta between January 2005 and May 2011. All patients had trouble walking. Follow-up examinations were performed with clinical visits; these included color Doppler ultrasonography and computed tomographic angiography. Technical, clinical, and hemodynamic success was achieved in all patients. None of the patients underwent reintervention during the follow-up period, which ranged from 3 months to 6 years (median 24 months). Nine complications occurred in six patients. Of the nine complications, four were distal thromboembolisms, which were successfully treated with catheter-directed thrombolysis or anticoagulation therapy. Endovascular treatment of the obstructive aortic disease using self-expanding stents was safe and effective, with high technical success and long-term patency. Thromboembolic complications were high even though direct stenting was considered protective for thromboembolism formation. Particularly for infrarenal aortic stenosis, it can be recommended as the first-line treatment option for patients with obstructive atherosclerotic aortic disease.

  10. Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis

    SciTech Connect (OSTI)

    Pauly, Markus; Sorensen, Susanne Oxenboll; Harholt, Jesper; Geshi, Naomi; Sakuragi, Yumiko; Moller, Isabel; Zandleven, Joris; Bernal, Adriana J.; Jensen, Niels Bjerg; Sorensen, Charlotte; Jensen, Jacob K.; Beldman, Gerrit; Willats, William G.T.; Scheller, Henrik

    2009-08-19

    Xylogalacturonan (XGA) is a class of pectic polysaccharide found in plant cell walls. The Arabidopsis thaliana locus At5g33290 encodes a predicted Type II membrane protein, and insertion mutants of the At5g33290 locus had decreased cell wall xylose. Immunological studies, enzymatic extraction of polysaccharides, monosaccharide linkage analysis, and oligosaccharide mass profiling were employed to identify the affected cell wall polymer. Pectic XGA was reduced to much lower levels in mutant than in wild-type leaves, indicating a role of At5g33290 in XGA biosynthesis. The mutated gene was designated xylogalacturonan deficient1 (xgd1). Transformation of the xgd1-1 mutant with the wild-type gene restored XGA to wild-type levels. XGD1 protein heterologously expressed in Nicotiana benthamiana catalyzed the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. The products formed could be hydrolyzed with an XGA-specific hydrolase. These results confirm that the XGD1 protein is a XGA xylosyltransferase. The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis.

  11. Extension of DOE N 206.5, Response and Notification Procedures for Data Breaches Involving Personally Identifiable Information

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2008-10-09

    This Notice extends DOE N 206.5, Response and Notification Procedures for Data Breaches Involving Personally Identifiable Information, dated 10-9-07, until 1-9-09.

  12. Consent Versus Consensus - Stakehold Involvement in the Identification of Necessary and Sufficient Transportation

    SciTech Connect (OSTI)

    Fawcett, Ricky Lee; Kramer, George Leroy Jr.

    2003-03-01

    Transportation (DOT) and the Nuclear Regulatory Commission (NRC) to provide for the protection of the public and the environment; historically these regulations have proven quite sufficient. Even so, when the Department of Energy (DOE) makes radioactive materials shipments, that are deemed to be a major federal activity, regulations under the National Environmental Policy Act require that public input on safety issues be sought. This requirement leads to interactions with State, Tribal and local stakeholders that often result in the imposition of extra-regulatory requirements requirements beyond those prescribed by DOT and NRC regulations. Unfortunately, these additional requirements virtually always increase costs and delay schedules, and usually do so without significantly increasing, and possibly even decreasing overall transportation safety. We believe that this problem arises because of efforts to achieve stakeholder consensus rather than stakeholder consent, where consensus connotes universal agreement with all aspects of the program, while consent, as used here, is simple agreement with the overall course of action. Gaining consensus entails extensive negotiations because all aspects and requirements of the project must be agreed to by each stakeholder. Gaining consent, on the other hand, requires only that stakeholders be satisfied that the project, as planned, provides adequately for their safety needs. This article addresses the issue of consent versus consensus and proposes a systematic, decision science process for reaching consent. Key steps in this proposed process are early identification and involvement of stakeholders, compilation of their concerns, perceptions, needs, causes, and translation of that information into an appropriate set of derived requirements. These derived requirements, along with already-established DOT and NRC regulatory requirements, form the necessary and sufficient conditions for safe transportation and for obtaining stakeholder consent.

  13. Public Involvement Plan Public Involvement Plan

    Energy Savers [EERE]

    ... U.S. Environmental Protection Agency FFB 61 Forsyth Street S.W. Atlanta, GA 30303-3104 EPA Hotline: 1-800-241-1745 EPA Public Affairs Fax: (404) 562-8174 Web site: www.epa.gov...

  14. Report on International Collaboration Involving the FE Heater and HG-A Tests at Mont Terri

    SciTech Connect (OSTI)

    Houseworth, Jim; Rutqvist, Jonny; Asahina, Daisuke; Chen, Fei; Vilarrasa, Victor; Liu, Hui-Hai; Birkholzer, Jens

    2013-11-06

    Nuclear waste programs outside of the US have focused on different host rock types for geological disposal of high-level radioactive waste. Several countries, including France, Switzerland, Belgium, and Japan are exploring the possibility of waste disposal in shale and other clay-rich rock that fall within the general classification of argillaceous rock. This rock type is also of interest for the US program because the US has extensive sedimentary basins containing large deposits of argillaceous rock. LBNL, as part of the DOE-NE Used Fuel Disposition Campaign, is collaborating on some of the underground research laboratory (URL) activities at the Mont Terri URL near Saint-Ursanne, Switzerland. The Mont Terri project, which began in 1995, has developed a URL at a depth of about 300 m in a stiff clay formation called the Opalinus Clay. Our current collaboration efforts include two test modeling activities for the FE heater test and the HG-A leak-off test. This report documents results concerning our current modeling of these field tests. The overall objectives of these activities include an improved understanding of and advanced relevant modeling capabilities for EDZ evolution in clay repositories and the associated coupled processes, and to develop a technical basis for the maximum allowable temperature for a clay repository. The R&D activities documented in this report are part of the work package of natural system evaluation and tool development that directly supports the following Used Fuel Disposition Campaign (UFDC) objectives: ? Develop a fundamental understanding of disposal-system performance in a range of environments for potential wastes that could arise from future nuclear-fuel-cycle alternatives through theory, simulation, testing, and experimentation. ? Develop a computational modeling capability for the performance of storage and disposal options for a range of fuel-cycle alternatives, evolving from generic models to more robust models of performance assessment. For the purpose of validating modeling capabilities for thermal-hydro-mechanical (THM) processes, we developed a suite of simulation models for the planned full-scale FE Experiment to be conducted in the Mont Terri URL, including a full three-dimensional model that will be used for direct comparison to experimental data once available. We performed for the first time a THM analysis involving the Barcelona Basic Model (BBM) in a full three-dimensional field setting for modeling the geomechanical behavior of the buffer material and its interaction with the argillaceous host rock. We have simulated a well defined benchmark that will be used for codeto- code verification against modeling results from other international modeling teams. The analysis highlights the complex coupled geomechanical behavior in the buffer and its interaction with the surrounding rock and the importance of a well characterized buffer material in terms of THM properties. A new geomechanical fracture-damage model, TOUGH-RBSN, was applied to investigate damage behavior in the ongoing HG-A test at Mont Terri URL. Two model modifications have been implemented so that the Rigid-Body-Spring-Network (RBSN) model can be used for analysis of fracturing around the HG-A microtunnel. These modifications are (1) a methodology to compute fracture generation under compressive stress conditions and (2) a method to represent anisotropic elastic and strength properties. The method for computing fracture generation under compressive load produces results that roughly follow trends expected for homogeneous and layered systems. Anisotropic properties for the bulk rock were represented in the RBSN model using layered heterogeneity and gave bulk material responses in line with expectations. These model improvements were implemented for an initial model of fracture damage at the HG-A test. While the HG-A test model results show some similarities with the test observations, differences between the model results and observations remain.

  15. The Bond Between CO and Cp?3U in Cp?3U(CO) involves Backbondingfrom the

    Office of Scientific and Technical Information (OSTI)

    Cp'3U Ligand-based Orbitals of ?pi-Symmetry, where Cp' Represents a Substituted Cyclopentadienyl Ligand. (Journal Article) | SciTech Connect The Bond Between CO and Cp?3U in Cp?3U(CO) involves Backbondingfrom the Cp'3U Ligand-based Orbitals of ?pi-Symmetry, where Cp' Represents a Substituted Cyclopentadienyl Ligand. Citation Details In-Document Search Title: The Bond Between CO and Cp?3U in Cp?3U(CO) involves Backbondingfrom the Cp'3U Ligand-based Orbitals of ?pi-Symmetry, where Cp'

  16. Involvement and Communication Committee.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Health, Safety, Environmental Protection Chair 1 : Becky Holland 4 , Member Vice Chair 1 : Richard Bloom 4 , Alternate Committee Members 2 : Richard Bloom Becky Holland Antone Brooks Steve Hudson 4 Jan Catrell Mike Korenko Lynn Davison Bob Legard Sam Dechter Margery Swint Dirk Dunning Unofficial Committee Members or Other Interested Parties 3 : Pam Larsen Susan Leckband 4 Mike Priddy Facilitator: Cathy McCague 4 Agency & Technical Support: Kris Holmes 4 DOE-RL Beth Rochette 4 Ecology Dieter

  17. Involvement and Communication Committee.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    River and Plateau Committee Chair 1 : Pam Larsen 4 , Member Vice Chair 1 : Jan Catrell 4 , Alternate Committee Members 2 : Don Bouchey Liz Mattson Jan Catrell Emmett Moore Shelley Cimon Jerry Peltier Shannon Cram Gerry Pollet Rob Davis Ed Revell Dale Engstrom Dan Serres Gary Garnant Dick Smith Floyd Hodges Bob Suyama John Howieson Gene Van Liew Alex Klementiev Jean Vanni Pam Larsen Dawn Wellman Susan Leckband 4 Steve White Larry Lockrem Unofficial Committee Members or Other Interested Parties 3

  18. Involvement and Communication Committee.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tank Waste Committee Chair 1 : Bob Suyama 4 , Member Vice Chair 1 : Melanie Meyers 4 , Member Committee Members 2 : David Bernhard Pam Larsen Don Bouchey Larry Lockrem Amoret Bunn...

  19. Involvement and Communication Committee.

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Budget and Contracts Committee Chair 1 : Jerry Peltier 4 , Member Vice Chair 1 : Ed Revell 4 , Member Committee Members 2 : Gary Garnant Gerry Pollet Susan Leckband 4 Ed Revell Ken...

  20. De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cárdenas-Conejo, Yair; Carballo-Uicab, Víctor; Lieberman, Meric; Aguilar-Espinosa, Margarita; Comai, Luca; Rivera-Madrid, Renata

    2015-10-28

    Bixin or annatto is a commercially important natural orange-red pigment derived from lycopene that is produced and stored in seeds of Bixa orellana L. An enzymatic pathway for bixin biosynthesis was inferred from homology of putative proteins encoded by differentially expressed seed cDNAs. Some activities were later validated in a heterologous system. Nevertheless, much of the pathway remains to be clarified. For example, it is essential to identify the methylerythritol phosphate (MEP) and carotenoid pathways genes. In order to investigate the MEP, carotenoid, and bixin pathways genes, total RNA from young leaves and two different developmental stages of seeds frommore » B. orellana were used for the construction of indexed mRNA libraries, sequenced on the Illumina HiSeq 2500 platform and assembled de novo using Velvet, CLC Genomics Workbench and CAP3 software. A total of 52,549 contigs were obtained with average length of 1,924 bp. Two phylogenetic analyses of inferred proteins, in one case encoded by thirteen general, single-copy cDNAs, in the other from carotenoid and MEP cDNAs, indicated that B. orellana is closely related to sister Malvales species cacao and cotton. Using homology, we identified 7 and 14 core gene products from the MEP and carotenoid pathways, respectively. Surprisingly, previously defined bixin pathway cDNAs were not present in our transcriptome. Here we propose a new set of gene products involved in bixin pathway. In conclusion, the identification and qRT-PCR quantification of cDNAs involved in annatto production suggest a hypothetical model for bixin biosynthesis that involve coordinated activation of some MEP, carotenoid and bixin pathway genes. These findings provide a better understanding of the mechanisms regulating these pathways and will facilitate the genetic improvement of B. orellana.« less

  1. Microsoft Word - PNNL 24125 Probative Investigation of the Thermal Stability of Wastes Involved in February 2014 WIPP Waste Dru

    Office of Environmental Management (EM)

    PART 970.doc Microsoft Word - PART 970.doc PDF icon Microsoft Word - PART 970.doc More Documents & Publications Searchable Electronic Department of Energy Acquisition Regulation Microsoft Word - Matrixpart2.doc AcqGuide70.52Matrixpart2Redo-OPAM

    PNNL-24125 Limited Distribution Probative Investigation of the Thermal Stability of Wastes Involved in February 2014 WIPP Waste Drum Breach Event March 2015 RD Scheele JM Schwantes BK McNamara MJ Minette DT Hobbs (SRNL) CA Barrett PNNL-24125

  2. Involved-Site Image-Guided Intensity Modulated Versus 3D Conformal Radiation Therapy in Early Stage Supradiaphragmatic Hodgkin Lymphoma

    SciTech Connect (OSTI)

    Filippi, Andrea Riccardo; Ciammella, Patrizia; Piva, Cristina; Ragona, Riccardo; Botto, Barbara; Gavarotti, Paolo; Merli, Francesco; Vitolo, Umberto; Iotti, Cinzia; Ricardi, Umberto

    2014-06-01

    Purpose: Image-guided intensity modulated radiation therapy (IG-IMRT) allows for margin reduction and highly conformal dose distribution, with consistent advantages in sparing of normal tissues. The purpose of this retrospective study was to compare involved-site IG-IMRT with involved-site 3D conformal RT (3D-CRT) in the treatment of early stage Hodgkin lymphoma (HL) involving the mediastinum, with efficacy and toxicity as primary clinical endpoints. Methods and Materials: We analyzed 90 stage IIA HL patients treated with either involved-site 3D-CRT or IG-IMRT between 2005 and 2012 in 2 different institutions. Inclusion criteria were favorable or unfavorable disease (according to European Organization for Research and Treatment of Cancer criteria), complete response after 3 to 4 cycles of an adriamycin- bleomycin-vinblastine-dacarbazine (ABVD) regimen plus 30 Gy as total radiation dose. Exclusion criteria were chemotherapy other than ABVD, partial response after ABVD, total radiation dose other than 30 Gy. Clinical endpoints were relapse-free survival (RFS) and acute toxicity. Results: Forty-nine patients were treated with 3D-CRT (54.4%) and 41 with IG-IMRT (45.6%). Median follow-up time was 54.2 months for 3D-CRT and 24.1 months for IG-IMRT. No differences in RFS were observed between the 2 groups, with 1 relapse each. Three-year RFS was 98.7% for 3D-CRT and 100% for IG-IMRT. Grade 2 toxicity events, mainly mucositis, were recorded in 32.7% of 3D-CRT patients (16 of 49) and in 9.8% of IG-IMRT patients (4 of 41). IG-IMRT was significantly associated with a lower incidence of grade 2 acute toxicity (P=.043). Conclusions: RFS rates at 3 years were extremely high in both groups, albeit the median follow-up time is different. Acute tolerance profiles were better for IG-IMRT than for 3D-CRT. Our preliminary results support the clinical safety and efficacy of advanced RT planning and delivery techniques in patients affected with early stage HL, achieving complete response after ABVD-based chemotherapy.

  3. Genome-Wide Identification and 3D Modeling of Proteins involved in DNA Damage Recognition and Repair (Final Report)

    SciTech Connect (OSTI)

    Ruben A. Abagyan, PhD

    2004-04-15

    OAK-B135 DNA Damage Recognition and Repair (DDR and R) proteins play a critical role in cellular responses to low-dose radiation and are associated with cancer. the authors have performed a systematic, genome-wide computational analysis of genomic data for human genes involved in the DDR and R process. The significant achievements of this project include: (1) Construction of the computational pipeline for searching DDR and R genes, building and validation of 3D models of proteins involved in DDR and R; (2) Functional and structural annotation of the 3D models and generation of comprehensive lists of suggested knock-out mutations; (3) Important improvement of macromolecular docking technology and its application to predict the DNA-Protein complex conformation; (4) Development of a new algorithm for improved analysis of high-density oligonucleotide arrays for gene expression profiling; (5) Construction and maintenance of the DNA Damage Recognition and Repair Database; and (6) Producing 14 research papers (10 published and 4 in preparation).

  4. Genome-Wide Identification and 3D Modeling of Proteins involved in DNA Damage Recognition and Repair (Final Report)

    SciTech Connect (OSTI)

    Abagyan, Ruben; An, Jianghong

    2005-08-12

    DNA Damage Recognition and Repair (DDR&R) proteins play a critical role in cellular responses to low-dose radiation and are associated with cancer. We have performed a systematic, genome-wide computational analysis of genomic data for human genes involved in the DDR&R process. The significant achievements of this project include: 1) Construction of the computational pipeline for searching DDR&R genes, building and validation of 3D models of proteins involved in DDR&R; 2) Functional and structural annotation of the 3D models and generation of comprehensive lists of suggested knock-out mutations; and the development of a method to predict the effects of mutations. Large scale testing of technology to identify novel small binding pockets in protein structures leading to new DDRR inhibitor strategies 3) Improvements of macromolecular docking technology (see the CAPRI 1-3 and 4-5 results) 4) Development of a new algorithm for improved analysis of high-density oligonucleotide arrays for gene expression profiling; 5) Construction and maintenance of the DNA Damage Recognition and Repair Database; 6) Producing 15 research papers (12 published and 3 in preparation).

  5. Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

    SciTech Connect (OSTI)

    Nagane, Masaki; Yasui, Hironobu; Sakai, Yuri; Yamamori, Tohru; Niwa, Koichi; Hattori, Yuichi; Kondo, Takashi; Inanami, Osamu

    2015-01-02

    Highlights: eNOS activity is increased in BAECs exposed to X-rays. ATM is involved in this increased eNOS activity. HSP90 modulates the radiation-induced activation of ATM and eNOS. - Abstract: In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90.

  6. DNA rearrangement in human follicular lymphoma can involve the 5' or the 3' region of the bcl-2 gene

    SciTech Connect (OSTI)

    Tsujimoto, Y.; Bashir, M.M.; Givol, I.; Cossman, J.; Jaffe, E.; Croce, C.M.

    1987-03-01

    In most human lymphomas, the chromosome translocation t(14;18) occurs within two breakpoint clustering regions on chromosome 18, the major one at the 3' untranslated region of the bcl-2 gene and the minor one at 3' of the gene. Analysis of a panel of follicular lymphoma DNAs using probes for the first exon of the bcl-2 gene indicates that DNA rearrangements may also occur 5' to the involved bcl-2 gene. In this case the IgH locus and the bcl-2 gene are found in an order suggesting that an inversion also occurred during the translocation process. The coding region of the bcl-2 gene, however, are left intact in all cases of follicular lymphoma studied to date.

  7. DOE Technical Standards List. Directory of DOE and contractor personnel involved in non-government standards activities

    SciTech Connect (OSTI)

    1997-06-01

    This is a periodic report on the level of agency participation in non-Government standards activities. This technical standards list is intended to assist US Department of Energy (DOE) management and other personnel involved in the DOE technical Standards Program by identifying those participating individuals. The body of this document contains a listing of DOE employees and DOE contractors who have submitted a Record of Non-Government Standards Activity. Additional names were added from rosters supplied by non-Government standards bodies. Appendices to this document are provided to list the information by parent employment organization, by non-Government standards activity, and by the proper names of the non-Government standards organizations and committees.

  8. Expediting environmental cleanup--nationwide stakeholder involvement in U.S. Department of Energy`s plume focus area

    SciTech Connect (OSTI)

    McCabe, G.H.; Stein, S.L.; Serie, P.J.

    1995-12-01

    The U.S. Department of Energy (DOE) faces a major challenge in cleaning up its contaminated sites throughout the United States. One major area of concern is the plumes in soil and ground water, contaminated with a myriad of different pollutants. DOE recently established the Plume Focus Area to address these problems. The mission of the Plume Focus Area is to enhance the deployment of innovative technologies for containing and cleaning up contaminant plumes in ground water and soil at all DOE sites. By involving a range of stakeholders in the selection, demonstration, and evaluation of new technologies, the deployment of these technologies can be enhanced. Through this strategy, technology users join with other stakeholders to assess the appropriateness of new technologies for addressing plume contamination, and characterize the conditions under which those emerging technologies will be acceptable. If new plume cleanup technologies are to be deployable, they must improve on today`s baseline technologies. If sites and their stakeholders understood the technologies, recognize that their concerns are reflected in the evaluations and demonstrations, and participate in assessing how technology performance addresses their concerns, the likelihood of acceptance of those technologies is greater. Thus, broad stakeholder acceptance becomes part of the definition of an improved technology, evaluated in parallel with technical performance, cost, and other traditional parameters. This paper further describes the goals and objectives of the Plume Focus Area and emphasizes the importance of stakeholder involvement in achieving them. The process of coordinating with DOE sites is described to highlight how stakeholder input is considered throughout the Plume Focus Area decision-making process in selecting, developing, demonstrating, and evaluating innovative technologies to address plume problems.

  9. The Orosomucoid 1 protein is involved in the vitamin D – mediated macrophage de-activation process

    SciTech Connect (OSTI)

    Gemelli, Claudia; Martello, Andrea; Montanari, Monica; Zanocco Marani, Tommaso; Salsi, Valentina; Zappavigna, Vincenzo; Parenti, Sandra; Vignudelli, Tatiana; Selmi, Tommaso; Ferrari, Sergio; Grande, Alexis

    2013-12-10

    Orosomucoid 1 (ORM1), also named Alpha 1 acid glycoprotein A (AGP-A), is an abundant plasma protein characterized by anti-inflammatory and immune-modulating properties. The present study was designed to identify a possible correlation between ORM1 and Vitamin D3 (1,25(OH)2D3), a hormone exerting a widespread effect on cell proliferation, differentiation and regulation of the immune system. In particular, the data described here indicated that ORM1 is a 1,25(OH)2D3 primary response gene, characterized by the presence of a VDRE element inside the 1 kb sequence of its proximal promoter region. This finding was demonstrated with gene expression studies, Chromatin Immunoprecipitation and luciferase transactivation experiments and confirmed by VDR full length and dominant negative over-expression. In addition, several experiments carried out in human normal monocytes demonstrated that the 1,25(OH)2D3 – VDR – ORM1 pathway plays a functional role inside the macrophage de-activation process and that ORM1 may be considered as a signaling molecule involved in the maintenance of tissue homeostasis and remodeling. - Highlights: • ORM1 is a Vitamin D primary response gene. • VD and its receptor VDR are involved in the de-activation process mediated by human resident macrophages. • The signaling pathway VD-VDR-ORM1 plays an important role in the control of macrophage de-activation process. • ORM1 may be defined as a signaling molecule implicated in the maintenance of tissue homeostasis and remodeling.

  10. Selection of potential IAEA inspection strategies involving cascade access at the Portsmouth Gas Centrifuge Enrichment Plant (GCEP)

    SciTech Connect (OSTI)

    Not Available

    1981-04-13

    This report has been prepared as a US contribution to Team 4 of the Hexapartite Safeguards Project. It provides to the Team 4 participants one example of an approach, which has been used in the United States, to developing a range of safeguards strategies involving differing degrees of access to cascade areas of centrifuge enrichment plants. Its purpose is to facilitate the work of other Hexapartite participants in completing Task II of Team 4's terms of reference. The scope of this report is limited to identifying safeguards approaches for the Portsmouth Gas Centrifuge Enrichment Plant (GCEP) which involve differing degrees of access to the cascade area. This report provides a method for selecting cascade access inspection strategies at GCEP which appear promising for more detailed evaluation. It is quite important to note, however, that the effectiveness and practicability of these strategies have not been established at the present. In addition, some strategies have been included on the basis of very preliminary calculations and considerations which have not been validated. Thus, some of these strategies may ultimately be rejected because they prove to be impracticable. Considerations of cost and the possible transfer of information and technology related to the production of enriched uranium will also be pertinent in considering the degrees and frequency of access to the cascade areas of centrifuge enrichment plants. This report describes the process for combining technical measures, implementation approaches and objectives to arrive at the total number of theoretically possible combinations. It then describes how these combinations may be reduced in a series of steps to a number that is more manageable for detailed evaluation. The process is shown schematically.

  11. Novel mechanism for scavenging of hypochlorite involving a periplasmic methionine-rich peptide and methionine sulfoxide reductase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Melnyk, Ryan A.; Youngblut, Matthew D.; Clark, Iain C.; Carlson, Hans K.; Wetmore, Kelly M.; Price, Morgan N.; Lavarone, Anthony T.; Deutschbauer, Adam M.; Arkin, Adam P.; Coates, John D.

    2015-05-12

    Reactive chlorine species (RCS) defense mechanisms are important for bacterial fitness in diverse environments. In addition to the anthropogenic use of RCS in the form of bleach, these compounds are also produced naturally through photochemical reactions of natural organic matter and in vivo by the mammalian immune system in response to invading microorganisms. To gain insight into bacterial RCS defense mechanisms, we investigated Azospira suillum strain PS, which produces periplasmic RCS as an intermediate of perchlorate respiration. Our studies identified an RCS response involving an RCS stress-sensing sigma/anti-sigma factor system (SigF/NrsF), a soluble hypochlorite-scavenging methionine-rich periplasmic protein (MrpX), and amore » putative periplasmic methionine sulfoxide reductase (YedY1). We investigated the underlying mechanism by phenotypic characterization of appropriate gene deletions, chemogenomic profiling of barcoded transposon pools, transcriptome sequencing, and biochemical assessment of methionine oxidation. Our results demonstrated that SigF was specifically activated by RCS and initiated the transcription of a small regulon centering around yedY1 and mrpX. A yedY1 paralog (yedY2) was found to have a similar fitness to yedY1 despite not being regulated by SigF. Markerless deletions of yedY2 confirmed its synergy with the SigF regulon. MrpX was strongly induced and rapidly oxidized by RCS, especially hypochlorite. Our results suggest a mechanism involving hypochlorite scavenging by sacrificial oxidation of the MrpX in the periplasm. Reduced MrpX is regenerated by the YedY methionine sulfoxide reductase activity. The phylogenomic distribution of this system revealed conservation in several Proteobacteria of clinical importance, including uropathogenic Escherichia coli and Brucella spp., implying a putative role in immune response evasion in vivo. In addition, bacteria are often stressed in the environment by reactive chlorine species (RCS) of either anthropogenic or natural origin, but little is known of the defense mechanisms they have evolved. Using a microorganism that generates RCS internally as part of its respiratory process allowed us to uncover a novel defense mechanism based on RCS scavenging by reductive reaction with a sacrificial methionine-rich peptide and redox recycling through a methionine sulfoxide reductase. As a result, this system is conserved in a broad diversity of organisms, including some of clinical importance, invoking a possible important role in innate immune system evasion.« less

  12. Novel mechanism for scavenging of hypochlorite involving a periplasmic methionine-rich peptide and methionine sulfoxide reductase

    SciTech Connect (OSTI)

    Melnyk, Ryan A.; Youngblut, Matthew D.; Clark, Iain C.; Carlson, Hans K.; Wetmore, Kelly M.; Price, Morgan N.; Lavarone, Anthony T.; Deutschbauer, Adam M.; Arkin, Adam P.; Coates, John D.

    2015-05-12

    Reactive chlorine species (RCS) defense mechanisms are important for bacterial fitness in diverse environments. In addition to the anthropogenic use of RCS in the form of bleach, these compounds are also produced naturally through photochemical reactions of natural organic matter and in vivo by the mammalian immune system in response to invading microorganisms. To gain insight into bacterial RCS defense mechanisms, we investigated Azospira suillum strain PS, which produces periplasmic RCS as an intermediate of perchlorate respiration. Our studies identified an RCS response involving an RCS stress-sensing sigma/anti-sigma factor system (SigF/NrsF), a soluble hypochlorite-scavenging methionine-rich periplasmic protein (MrpX), and a putative periplasmic methionine sulfoxide reductase (YedY1). We investigated the underlying mechanism by phenotypic characterization of appropriate gene deletions, chemogenomic profiling of barcoded transposon pools, transcriptome sequencing, and biochemical assessment of methionine oxidation. Our results demonstrated that SigF was specifically activated by RCS and initiated the transcription of a small regulon centering around yedY1 and mrpX. A yedY1 paralog (yedY2) was found to have a similar fitness to yedY1 despite not being regulated by SigF. Markerless deletions of yedY2 confirmed its synergy with the SigF regulon. MrpX was strongly induced and rapidly oxidized by RCS, especially hypochlorite. Our results suggest a mechanism involving hypochlorite scavenging by sacrificial oxidation of the MrpX in the periplasm. Reduced MrpX is regenerated by the YedY methionine sulfoxide reductase activity. The phylogenomic distribution of this system revealed conservation in several Proteobacteria of clinical importance, including uropathogenic Escherichia coli and Brucella spp., implying a putative role in immune response evasion in vivo. In addition, bacteria are often stressed in the environment by reactive chlorine species (RCS) of either anthropogenic or natural origin, but little is known of the defense mechanisms they have evolved. Using a microorganism that generates RCS internally as part of its respiratory process allowed us to uncover a novel defense mechanism based on RCS scavenging by reductive reaction with a sacrificial methionine-rich peptide and redox recycling through a methionine sulfoxide reductase. As a result, this system is conserved in a broad diversity of organisms, including some of clinical importance, invoking a possible important role in innate immune system evasion.

  13. Visible light stimulating dual-wavelength emission and O vacancy involved energy transfer behavior in luminescence for coaxial nanocable arrays

    SciTech Connect (OSTI)

    Yang, Lei, E-mail: nanoyang@qq.com [College of Materials Science and Engineering, Hunan University, Changsha 410082 (China); Hunan Province Key Laboratory for Spray Deposition Technology and Application, Hunan University, Changsha 410082 (China); Dong, Jiazhang; Jiang, Zhongcheng [College of Materials Science and Engineering, Hunan University, Changsha 410082 (China); Pan, Anlian; Zhuang, Xiujuan [Key Laboratory for Micro-Nano Physics and Technology of Hunan Province, Hunan University, Changsha 410082 (China)

    2014-06-14

    We report a strategy to investigate O vacancy (V{sub O}) involved energy transfer and dual-wavelength yellow emission in coaxial nanocable. By electric field deposition and subsequent sol-gel template approach, ZnO:Tb/Y{sub 2}O{sub 3}:Eu coaxial nanocable arrays are synthesized. After visible light excitation, system is promoted to O vacancy charge transfer state of V{sub O}(0/+). In the following cross relaxation, energy transfer from V{sub O} to the excitation energy level of Tb{sup 3+} in ZnO:Tb core area. While in Y{sub 2}O{sub 3}:Eu shell area, energy transfer to the excitation energy level of Eu{sup 3+}. Subsequently, dual-wavelength emission is observed. By constructing nanocable with dual-wavelength emission, yellow luminescence is obtained. Adjust doping concentration of Eu{sup 3+} or Tb{sup 3+} in the range of 0.010.05, chromaticity coordinates of ZnO:Tb/Y{sub 2}O{sub 3}:Eu nanocable stably stays at yellow region in color space except ZnO:Tb{sub 0.01}/Y{sub 2}O{sub 3}:Eu{sub 0.01}. As Vo states act as media in energy transfer process in nanocablers, visible light can stimulate dual-wavelength emissions. Yellow luminescent nanocable arrays will have great applications in light-emitting diode luminescence.

  14. G-protein-coupled estrogen receptor 1 is involved in brain development during zebrafish (Danio rerio) embryogenesis

    SciTech Connect (OSTI)

    Shi, Yanan; Liu, Xiaochun; Zhu, Pei; Li, Jianzhen; Sham, Kathy W.Y.; Cheng, Shuk Han; Li, Shuisheng; Zhang, Yong; Cheng, Christopher H.K.; Lin, Haoran; College of Ocean, Hainan University, Haikou 570228, Hainan

    2013-05-24

    Highlights: •The Gper expression was detected in the developing brain of zebrafish. •Gper morpholino knockdown induced apoptosis of brain cells. •Gper morpholino knockdown reduced expression in neuron markers. •Zebrafish Gper may be involved in neuronal development. -- Abstract: G-protein-coupled estrogen receptor 1 (Gper, formerly known as GPR30) is found to be a trophic and protective factor in mediating action of estrogen in adult brain, while its role in developing brain remains to be elucidated. Here we present the expression pattern of Gper and its functions during embryogenesis in zebrafish. Both the mRNA and protein of Gper were detected throughout embryogenesis. Whole mount in situ hybridization (WISH) revealed a wide distribution of gper mRNAs in various regions of the developing brain. Gper knockdown by specific morpholinos resulted in growth retardation in embryos and morphological defects in the developing brain. In addition, induced apoptosis, decreased proliferation of the brain cells and maldevelopment of sensory and motor neurons were also found in the morphants. Our results provide novel insights into Gper functions in the developing brain, revealing that Gper can maintain the survival of the brain cells, and formation and/or differentiation of the sensory and motor neurons.

  15. Nuclear substructure reorganization during late stageerythropoiesis is selective and does not involve caspase cleavage ofmajor nuclear substructural proteins

    SciTech Connect (OSTI)

    Krauss, Sharon Wald; Lo, Annie J.; Short, Sarah A.; Koury, MarkJ.; Mohandas, Narla; Chasis, Joel Anne

    2005-04-06

    Enucleation, a rare feature of mammalian differentiation, occurs in three cell types: erythroblasts, lens epithelium and keratinocytes. Previous investigations suggest that caspase activation functions in lens epithelial and keratinocyte enucleation, as well as in early erythropoiesis encompassing BFU-E differentiation to proerythroblast. To determine whether caspase activation contributes to later erythropoiesis and whether nuclear substructures other than chromatin reorganize, we analyzed distributions of nuclear subcompartment proteins and assayed for caspase-induced cleavage of subcompartmental target proteins in mouse erythroblasts. We found that patterns of lamin B in the filamentous network interacting with both the nuclear envelope and DNA, nuclear matrix protein NuMA, and splicing factors Sm and SC35 persisted during nuclear condensation, consistent with effective transcription of genes expressed late in differentiation. Thus nuclear reorganization prior to enucleation is selective, allowing maintenance of critical transcriptional processes independent of extensive chromosomal reorganization. Consistent with these data, we found no evidence for caspase-induced cleavage of major nuclear subcompartment proteins during late erythropoiesis, in contrast to what has been observed in early erythropoiesis and in lens epithelial and keratinocyte differentiation. These findings imply that nuclear condensation and extrusion during terminal erythroid differentiation involve novel mechanisms that do not entail major activation of apoptotic machinery.

  16. H2-M polymorphism in mice susceptible to collagen-induced arthritis involves the peptide binding groove

    SciTech Connect (OSTI)

    Walter, W.; Loos, M.; Maeurer, M.J.

    1996-12-31

    The ability to develop type II collagen (CII)-induced arthritis (CIA) in mice is associated with the major histocompatibility I-A gene and with as yet poorly defined regulatory molecules of the major histocompatibility complex (MHC) class II antigen processing and presentation pathway. H2-M molecules are thought to be involved in the loading of antigenic peptides into the MHC class II binding cleft. We sequenced H2-Ma, H2-Mb1, and H2-Mb2 genes from CIA-susceptible and -resistant mouse strains and identified four different Ma and Mb2 alleles, and three different Mb1 alleles defined by polymorphic residues within the predicted peptide binding groove. Most CIA-resistant mouse strains share common Ma, Mb1, and Mb2 alleles. In contrast, H2-M alleles designated Ma-III, Ma-IV, Mb1-III, and Mb2-IV could be exclusively identified in the CIA-susceptible H2{sup r} and H2{sup q} haplotypes, suggesting that allelic H2-M molecules may modulate the composition of different CII peptides loaded onto MHC class II molecules, presumably presenting {open_quotes}arthritogenic{close_quotes} epitopes to T lymphocytes. 42 refs., 4 figs., 3 tabs.

  17. Evidence of extra-telomeric effects of hTERT and its regulation involving a feedback loop

    SciTech Connect (OSTI)

    Lai, Serene R.; Cunningham, Amanda P.; Huynh, Vu Q.; Andrews, Lucy G.; Tollefsbol, Trygve O. . E-mail: trygve@uab.edu

    2007-01-15

    The human telomerase reverse transcriptase (hTERT) is the catalytic subunit of the enzyme telomerase which is responsible for telomeric maintenance and extension. Using RNA interference to knock down hTERT mRNA expression, we provide evidence that hTERT exerts extra-telomeric effects on the cell cycle and on its own regulatory proteins, specifically: p53 and p21. We tested our hypothesis that hTERT regulates its own expression through effects on upstream regulatory genes using transformed human embryonic kidney (HEK 293) cells, p53 and p16 {sup INK4a} null human ovarian cancer SKOV-3 cells, and p53-null MDA-MB-157 human mammary cancer cells. In HEK 293 cells, hTERT knockdown resulted in elevated p53 and p21 transcription and a decrease in cellular proliferation. Similar results were observed in the MDA-MB-157 cell line where p21 was upregulated, correlating with cell growth inhibition. In contrast, we observed a decrease in expression of p21 in SKOV-3 cells with hTERT knockdown and cell growth appeared to be unaffected. These findings suggest that hTERT may be involved in a feedback loop system, thereby playing a role in its own regulation.

  18. Findings of an evaluation of public involvement programs associated with the development of a Land and Resource Management Plan for the Ouachita National Forest

    SciTech Connect (OSTI)

    Holthoff, M.G.; Howell, R.E.

    1993-08-01

    Federal regulations require the United States Forest Service (USFS) to integrate public input and values into decisions concerning land and resource management planning. The USFS has typically relied on traditional methods of involving the public, whereby public access and input to policy development are unilaterally controlled by the agency. Because of the highly political nature of land and resource management planning, such technocratic forms of public involvement and decision-making appear to be proving ineffective. This paper describes and evaluates two public involvement programs associated with the Ouachita National Forest`s (ONF) lengthy forest planning process. The research consisted of personal interviews with key program leaders and knowledgeable citizen participants, collection of secondary data, and a survey of citizen participants. Because of controversial planning decisions made during an initial planning process, the ONF was forced to re-enter the planning process in order to address unresolved planning issues and to conduct a more effective public involvement program. The supplemental planning process also resulted in a considerable degree of public contention. The survey revealed that although citizen participants were somewhat more satisfied with the supplemental public involvement program relative to the initial program, neither program was viewed as satisfactory. The findings of the study suggest that in order to be more effective, USFS public involvement programs should be more responsive to public concerns and conducted in adherence to principles of collaborative planning.

  19. Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sieber, Jessica R.; Crable, Bryan R.; Sheik, Cody S.; Hurst, Gregory B.; Rohlin, Lars; Gunsalus, Robert P.; McInerney, Michael J.

    2015-02-11

    We report that microbial syntrophy is a vital metabolic interaction necessary for the complete oxidation of organic biomass to methane in all-anaerobic ecosystems. However, this process is thermodynamically constrained and represents an ecosystem-level metabolic bottleneck. To gain insight into the physiology of this process, a shotgun proteomics approach was used to quantify the protein landscape of the model syntrophic metabolizer, Syntrophomonas wolfei, grown axenically and syntrophically with Methanospirillum hungatei. Remarkably, the abundance of most proteins as represented by normalized spectral abundance factor (NSAF) value changed very little between the pure and coculture growth conditions. Among the most abundant proteins detectedmore » were GroEL and GroES chaperonins, a small heat shock protein, and proteins involved in electron transfer, beta-oxidation, and ATP synthesis. Several putative energy conservation enzyme systems that utilize NADH and ferredoxin were present. The abundance of an EtfAB2 and the membrane-bound iron-sulfur oxidoreductase (Swol_0698 gene product) delineated a potential conduit for electron transfer between acyl-CoA dehydrogenases and membrane redox carriers. Proteins detected only when S. wolfei was grown with M. hungatei included a zinc-dependent dehydrogenase with a GroES domain, whose gene is present in genomes in many organisms capable of syntrophy, and transcriptional regulators responsive to environmental stimuli or the physiological status of the cell. In conclusion, the proteomic analysis revealed an emphasis on macromolecular stability and energy metabolism by S. wolfei and presence of regulatory mechanisms responsive to external stimuli and cellular physiological status.« less

  20. Mitochondrial remodeling following fission inhibition by 15d-PGJ2 involves molecular changes in mitochondrial fusion protein OPA1

    SciTech Connect (OSTI)

    Kar, Rekha; Department of Biochemistry, UT Health Science Center at San Antonio, San Antonio, TX 78229 ; Mishra, Nandita; Singha, Prajjal K.; Venkatachalam, Manjeri A.; Department of Biochemistry, UT Health Science Center at San Antonio, San Antonio, TX 78229 ; Saikumar, Pothana

    2010-09-03

    Research highlights: {yields} Chemical inhibition of fission protein Drp1 leads to mitochondrial fusion. {yields} Increased fusion stimulates molecular changes in mitochondrial fusion protein OPA1. {yields} Proteolysis of larger isoforms, new synthesis and ubiquitination of OPA1 occur. {yields} Loss of mitochondrial tubular rigidity and disorganization of cristae. {yields} Generation of large swollen dysfunctional mitochondria. -- Abstract: We showed earlier that 15 deoxy {Delta}{sup 12,14} prostaglandin J2 (15d-PGJ2) inactivates Drp1 and induces mitochondrial fusion . However, prolonged incubation of cells with 15d-PGJ2 resulted in remodeling of fused mitochondria into large swollen mitochondria with irregular cristae structure. While initial fusion of mitochondria by 15d-PGJ2 required the presence of both outer (Mfn1 and Mfn2) and inner (OPA1) mitochondrial membrane fusion proteins, later mitochondrial changes involved increased degradation of the fusion protein OPA1 and ubiquitination of newly synthesized OPA1 along with decreased expression of Mfn1 and Mfn2, which likely contributed to the loss of tubular rigidity, disorganization of cristae, and formation of large swollen degenerated dysfunctional mitochondria. Similar to inhibition of Drp1 by 15d-PGJ2, decreased expression of fission protein Drp1 by siRNA also resulted in the loss of fusion proteins. Prevention of 15d-PGJ2 induced mitochondrial elongation by thiol antioxidants prevented not only loss of OPA1 isoforms but also its ubiquitination. These findings provide novel insights into unforeseen complexity of molecular events that modulate mitochondrial plasticity.

  1. An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis

    SciTech Connect (OSTI)

    Dai, Jingcheng; Wei, Hehong; Tian, Chunyuan; Damron, Fredrick; Zhou, Jizhong; Qiu, Dongru

    2015-01-01

    Background: Bacteria use alternative sigma factors (σs) to regulate condition-specific gene expression for survival and Shewanella harbors multiple ECF (extracytoplasmic function) σ genes and cognate anti-sigma factor genes. Here we comparatively analyzed two of the rpoE-like operons in the strain MR-1: rpoE-rseA-rseB-rseC and rpoE2-chrR. Results: RpoE was important for bacterial growth at low and high temperatures, in the minimal medium, and high salinity. The degP/htrA orthologue, required for growth of Escherichia coli and Pseudomonas aeruginosa at high temperature, is absent in Shewanella, while the degQ gene is RpoE-regulated and is required for bacterial growth at high temperature. RpoE2 was essential for the optimal growth in oxidative stress conditions because the rpoE2 mutant was sensitive to hydrogen peroxide and paraquat. The operon encoding a ferrochelatase paralogue (HemH2) and a periplasmic glutathione peroxidase (PgpD) was identified as RpoE2-dependent. PgpD exhibited higher activities and played a more important role in the oxidative stress responses than the cytoplasmic glutathione peroxidase CgpD under tested conditions. The rpoE2-chrR operon and the identified regulon genes, including pgpD and hemH2, are coincidently absent in several psychrophilic and/or deep-sea Shewanella strains. Conclusion: In S. oneidensis MR-1, the RpoE-dependent degQ gene is required for optimal growth under high temperature. The rpoE2 and RpoE2-dependent pgpD gene encoding a periplasmic glutathione peroxidase are involved in oxidative stress responses. But rpoE2 is not required for bacterial growth at low temperature and it even affected bacterial growth under salt stress, indicating that there is a tradeoff between the salt resistance and RpoE2-mediated oxidative stress responses.

  2. An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dai, Jingcheng; Wei, Hehong; Tian, Chunyuan; Damron, Fredrick; Zhou, Jizhong; Qiu, Dongru

    2015-01-01

    Background: Bacteria use alternative sigma factors (σs) to regulate condition-specific gene expression for survival and Shewanella harbors multiple ECF (extracytoplasmic function) σ genes and cognate anti-sigma factor genes. Here we comparatively analyzed two of the rpoE-like operons in the strain MR-1: rpoE-rseA-rseB-rseC and rpoE2-chrR. Results: RpoE was important for bacterial growth at low and high temperatures, in the minimal medium, and high salinity. The degP/htrA orthologue, required for growth of Escherichia coli and Pseudomonas aeruginosa at high temperature, is absent in Shewanella, while the degQ gene is RpoE-regulated and is required for bacterial growth at high temperature. RpoE2 was essentialmore » for the optimal growth in oxidative stress conditions because the rpoE2 mutant was sensitive to hydrogen peroxide and paraquat. The operon encoding a ferrochelatase paralogue (HemH2) and a periplasmic glutathione peroxidase (PgpD) was identified as RpoE2-dependent. PgpD exhibited higher activities and played a more important role in the oxidative stress responses than the cytoplasmic glutathione peroxidase CgpD under tested conditions. The rpoE2-chrR operon and the identified regulon genes, including pgpD and hemH2, are coincidently absent in several psychrophilic and/or deep-sea Shewanella strains. Conclusion: In S. oneidensis MR-1, the RpoE-dependent degQ gene is required for optimal growth under high temperature. The rpoE2 and RpoE2-dependent pgpD gene encoding a periplasmic glutathione peroxidase are involved in oxidative stress responses. But rpoE2 is not required for bacterial growth at low temperature and it even affected bacterial growth under salt stress, indicating that there is a tradeoff between the salt resistance and RpoE2-mediated oxidative stress responses.« less

  3. The Impact of Extent and Location of Mediastinal Lymph Node Involvement on Survival in Stage III Non-Small Cell Lung Cancer Patients Treated With Definitive Radiotherapy

    SciTech Connect (OSTI)

    Fernandes, Annemarie T.; Mitra, Nandita; Xanthopoulos, Eric; Evans, Tracey; Stevenson, James; Langer, Corey; Kucharczuk, John C.; Lin, Lilie; Rengan, Ramesh

    2012-05-01

    Purpose: Several surgical series have identified subcarinal, contralateral, and multilevel nodal involvement as predictors of poor overall survival in patients with Stage III non-small-cell lung cancer (NSCLC) treated with definitive resection. This retrospective study evaluates the impact of extent and location of mediastinal lymph node (LN) involvement on survival in patients with Stage III NSCLC treated with definitive radiotherapy. Methods and Materials: We analyzed 106 consecutive patients with T1-4 N2-3 Stage III NSCLC treated with definitive radiotherapy at University of Pennsylvania between January 2003 and February 2009. For this analysis, mediastinal LN stations were divided into four mutually exclusive groups: supraclavicular, ipsilateral mediastinum, contralateral mediastinum, and subcarinal. Patients' conditions were then analyzed according to the extent of involvement and location of mediastinal LN stations. Results: The majority (88%) of patients received sequential or concurrent chemotherapy. The median follow-up time for survivors was 32.6 months. By multivariable Cox modeling, chemotherapy use (hazard ratio [HR]: 0.21 [95% confidence interval (CI): 0.07-0.63]) was associated with improved overall survival. Increasing primary tumor [18F]-fluoro-2-deoxy-glucose avidity (HR: 1.11 [CI: 1.06-1.19]), and subcarinal involvement (HR: 2.29 [CI: 1.11-4.73]) were significant negative predictors of overall survival. On univariate analysis, contralateral nodal involvement (HR: 0.70 [CI: 0.33-1.47]), supraclavicular nodal involvement (HR: 0.78 [CI: 0.38-1.67]), multilevel nodal involvement (HR: 0.97 [CI: 0.58-1.61]), and tumor size (HR: 1.04 [CI: 0.94-1.14]) did not predict for overall survival. Patients with subcarinal involvement also had lower rates of 2-year nodal control (51.2% vs. 74.9%, p = 0.047) and 2-year distant control (28.4% vs. 61.2%, p = 0.043). Conclusions: These data suggest that the factors that determine oncologic outcome in Stage III NSCLC patients treated with definitive radiotherapy are distinct from those observed in patients who undergo surgical resection. The ultimate efficacy of radiation in locally advanced NSCLC is dependent on the intrinsic biology of the tumor.

  4. An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica

    SciTech Connect (OSTI)

    Poopanitpan, Napapol; Kobayashi, Satoshi; Fukuda, Ryouichi; Horiuchi, Hiroyuki; Ohta, Akinori

    2010-11-26

    Research highlights: {yields} POR1 is a Yarrowia lipolytica ortholog of farA involved in fatty acid response in A. nidulans. {yields} Deletion of POR1 caused growth defects on fatty acids. {yields} {Delta}por1 strain exhibited defects in the induction of genes involved in fatty acid utilization. -- Abstract: The yeast Yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. To identify a gene(s) regulating fatty acid utilization in Y. lipolytica, we first studied homologous genes to OAF1 and PIP2 of Saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. We next characterized a Y. lipolytica gene, POR1 (primary oleate regulator 1), an ortholog of farA encoding a transcriptional activator that regulates fatty acid utilization in Aspergillus nidulans. The deletion mutant of POR1 was defective in the growth on various fatty acids, but not on glucose, glycerol, or n-hexadecane. It exhibited slight defect on n-decane. The transcriptional induction of genes involved in {beta}-oxidation and peroxisome proliferation by oleate was distinctly diminished in the {Delta}por1 strains. These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y. lipolytica.

  5. B Decays Involving Light Mesons

    SciTech Connect (OSTI)

    Eschrich, Ivo Gough; /UC, Irvine

    2007-01-09

    Recent BABAR results for decays of B-mesons to combinations of non-charm mesons are presented. This includes B decays to two vector mesons, B {yields} {eta}{prime}({pi}, K, {rho}) modes, and a comprehensive Dalitz Plot analysis of B {yields} KKK decays.

  6. Cyber Incidents Involving Control Systems

    SciTech Connect (OSTI)

    Robert J. Turk

    2005-10-01

    The Analysis Function of the US-CERT Control Systems Security Center (CSSC) at the Idaho National Laboratory (INL) has prepared this report to document cyber security incidents for use by the CSSC. The description and analysis of incidents reported herein support three CSSC tasks: establishing a business case; increasing security awareness and private and corporate participation related to enhanced cyber security of control systems; and providing informational material to support model development and prioritize activities for CSSC. The stated mission of CSSC is to reduce vulnerability of critical infrastructure to cyber attack on control systems. As stated in the Incident Management Tool Requirements (August 2005) ''Vulnerability reduction is promoted by risk analysis that tracks actual risk, emphasizes high risk, determines risk reduction as a function of countermeasures, tracks increase of risk due to external influence, and measures success of the vulnerability reduction program''. Process control and Supervisory Control and Data Acquisition (SCADA) systems, with their reliance on proprietary networks and hardware, have long been considered immune to the network attacks that have wreaked so much havoc on corporate information systems. New research indicates this confidence is misplaced--the move to open standards such as Ethernet, Transmission Control Protocol/Internet Protocol, and Web technologies is allowing hackers to take advantage of the control industry's unawareness. Much of the available information about cyber incidents represents a characterization as opposed to an analysis of events. The lack of good analyses reflects an overall weakness in reporting requirements as well as the fact that to date there have been very few serious cyber attacks on control systems. Most companies prefer not to share cyber attack incident data because of potential financial repercussions. Uniform reporting requirements will do much to make this information available to Department of Homeland Security (DHS) and others who require it. This report summarizes the rise in frequency of cyber attacks, describes the perpetrators, and identifies the means of attack. This type of analysis, when used in conjunction with vulnerability analyses, can be used to support a proactive approach to prevent cyber attacks. CSSC will use this document to evolve a standardized approach to incident reporting and analysis. This document will be updated as needed to record additional event analyses and insights regarding incident reporting. This report represents 120 cyber security incidents documented in a number of sources, including: the British Columbia Institute of Technology (BCIT) Industrial Security Incident Database, the 2003 CSI/FBI Computer Crime and Security Survey, the KEMA, Inc., Database, Lawrence Livermore National Laboratory, the Energy Incident Database, the INL Cyber Incident Database, and other open-source data. The National Memorial Institute for the Prevention of Terrorism (MIPT) database was also interrogated but, interestingly, failed to yield any cyber attack incidents. The results of this evaluation indicate that historical evidence provides insight into control system related incidents or failures; however, that the limited available information provides little support to future risk estimates. The documented case history shows that activity has increased significantly since 1988. The majority of incidents come from the Internet by way of opportunistic viruses, Trojans, and worms, but a surprisingly large number are directed acts of sabotage. A substantial number of confirmed, unconfirmed, and potential events that directly or potentially impact control systems worldwide are also identified. Twelve selected cyber incidents are presented at the end of this report as examples of the documented case studies (see Appendix B).

  7. Who's Involved | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    2014 DOE CFC Campaign Leadership Chair Ernest Moniz, Secretary of Energy Vice Chair Dot Harris, Director, Office of Economic Impact and Diversity Champion David M. Klaus, Deputy...

  8. Safety Success Through Employee Involvement

    Broader source: Energy.gov [DOE]

    Presenters: James Pollard, Monica Lewis, Michael Hendrix, Stacy Miller, Y-12 National Security Complex Track 2-2

  9. Classified Visits Involving Foreign Nationals

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2004-01-13

    The Order establishes a program to manage the Department-wide program to facilitate, document, and assure accountability when approving foreign national access to classified DOE programs and facilities. Cancels portions of Chapter VIII, of DOE O 470.1 that pertain to foreign nationals who visit DOE sitess and require access to classified information. Canceled by DOE O 470.4B.

  10. Tris(1,3-dichloro-2-propyl) phosphate perturbs the expression of genes involved in immune response and lipid and steroid metabolism in chicken embryos

    SciTech Connect (OSTI)

    Farhat, Amani; Buick, Julie K.; Williams, Andrew; Yauk, Carole L.; O'Brien, Jason M.; Crump, Doug; Williams, Kim L.; Chiu, Suzanne; Kennedy, Sean W.

    2014-03-01

    We previously demonstrated that in ovo exposure to the flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCPP) decreased plasma thyroxine levels, reduced growth parameters, and decreased gallbladder size in chicken embryos. In the current study DNA microarrays were used to evaluate global mRNA expression in liver tissue of male chicken embryos that exhibited the above mentioned effects. Injected doses were dimethyl sulfoxide vehicle control, 7.6 or 45 ?g TDCPP/g egg. TDCPP caused significant changes in the expression of five genes at the low dose and 47 genes at the high dose (False Discovery Rate p ? 0.1, fold change ? 1.5). The gene expression analysis suggested a compromised immune function, a state of cholestatic liver/biliary fibrosis, and disrupted lipid and steroid metabolism. Circulating bile acid levels were elevated, which is an indication of liver dysfunction, and plasma cholesterol levels were reduced; however, hepatic bile acid and cholesterol levels were unaltered. Interactome analyses identified apolipoprotein E, hepatocyte nuclear factor 4 alpha, and peroxisome proliferator-activated receptor alpha as key regulatory molecules involved in the effects of TDCPP. Our results demonstrate a targeted effect of TDCPP toxicity on lipid metabolism, including cholesterol, that helps explain the aforementioned phenotypic effects, as chicken embryos are highly dependent on yolk lipids for growth and maintenance throughout development. Finally, our results are in concordance with the literature that describes TDCPP as a cancer-causing agent, since the majority of dysregulated genes were involved in cancer pathways. - Highlights: TDCPP dysregulates genes involved in immune function and lipid metabolism. A targeted effect of TDCPP toxicity on cholesterol metabolism is apparent. A state of cholestatic liver fibrosis is suggested by the expression profile. Elevated plasma bile acids suggest that TDCPP causes liver dysfunction.

  11. Optimized Volumetric Modulated Arc Therapy Versus 3D-CRT for Early Stage Mediastinal Hodgkin Lymphoma Without Axillary Involvement: A Comparison of Second Cancers and Heart Disease Risk

    SciTech Connect (OSTI)

    Filippi, Andrea Riccardo; Ragona, Riccardo; Piva, Cristina; Scafa, Davide; Fiandra, Christian; Fusella, Marco; Giglioli, Francesca Romana; Lohr, Frank; Ricardi, Umberto

    2015-05-01

    Purpose: The purpose of this study was to evaluate the risks of second cancers and cardiovascular diseases associated with an optimized volumetric modulated arc therapy (VMAT) planning solution in a selected cohort of stage I/II Hodgkin lymphoma (HL) patients treated with either involved-node or involved-site radiation therapy in comparison with 3-dimensional conformal radiation therapy (3D-CRT). Methods and Materials: Thirty-eight patients (13 males and 25 females) were included. Disease extent was mediastinum alone (n=8, 21.1%); mediastinum plus unilateral neck (n=19, 50%); mediastinum plus bilateral neck (n=11, 29.9%). Prescription dose was 30 Gy in 2-Gy fractions. Only 5 patients had mediastinal bulky disease at diagnosis (13.1%). Anteroposterior 3D-CRT was compared with a multiarc optimized VMAT solution. Lung, breast, and thyroid cancer risks were estimated by calculating a lifetime attributable risk (LAR), with a LAR ratio (LAR{sub VMAT}-to-LAR{sub 3D-CRT}) as a comparative measure. Cardiac toxicity risks were estimated by calculating absolute excess risk (AER). Results: The LAR ratio favored 3D-CRT for lung cancer induction risk in mediastinal alone (P=.004) and mediastinal plus unilateral neck (P=.02) presentations. LAR ratio for breast cancer was lower for VMAT in mediastinal plus bilateral neck presentations (P=.02), without differences for other sites. For thyroid cancer, no significant differences were observed, regardless of anatomical presentation. A significantly lower AER of cardiac (P=.038) and valvular diseases (P<.0001) was observed for VMAT regardless of disease extent. Conclusions: In a cohort of patients with favorable characteristics in terms of disease extent at diagnosis (large prevalence of nonbulky presentations without axillary involvement), optimized VMAT reduced heart disease risk with comparable risks of thyroid and breast cancer, with an increase in lung cancer induction probability. The results are however strongly influenced by the different anatomical presentations, supporting an individualized approach.

  12. De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis

    SciTech Connect (OSTI)

    Cárdenas-Conejo, Yair; Carballo-Uicab, Víctor; Lieberman, Meric; Aguilar-Espinosa, Margarita; Comai, Luca; Rivera-Madrid, Renata

    2015-10-28

    Bixin or annatto is a commercially important natural orange-red pigment derived from lycopene that is produced and stored in seeds of Bixa orellana L. An enzymatic pathway for bixin biosynthesis was inferred from homology of putative proteins encoded by differentially expressed seed cDNAs. Some activities were later validated in a heterologous system. Nevertheless, much of the pathway remains to be clarified. For example, it is essential to identify the methylerythritol phosphate (MEP) and carotenoid pathways genes. In order to investigate the MEP, carotenoid, and bixin pathways genes, total RNA from young leaves and two different developmental stages of seeds from B. orellana were used for the construction of indexed mRNA libraries, sequenced on the Illumina HiSeq 2500 platform and assembled de novo using Velvet, CLC Genomics Workbench and CAP3 software. A total of 52,549 contigs were obtained with average length of 1,924 bp. Two phylogenetic analyses of inferred proteins, in one case encoded by thirteen general, single-copy cDNAs, in the other from carotenoid and MEP cDNAs, indicated that B. orellana is closely related to sister Malvales species cacao and cotton. Using homology, we identified 7 and 14 core gene products from the MEP and carotenoid pathways, respectively. Surprisingly, previously defined bixin pathway cDNAs were not present in our transcriptome. Here we propose a new set of gene products involved in bixin pathway. In conclusion, the identification and qRT-PCR quantification of cDNAs involved in annatto production suggest a hypothetical model for bixin biosynthesis that involve coordinated activation of some MEP, carotenoid and bixin pathway genes. These findings provide a better understanding of the mechanisms regulating these pathways and will facilitate the genetic improvement of B. orellana.

  13. As you may kn&<' the~de&tment of &~er& (D&j 1s involved'in'a pronram

    Office of Legacy Management (LM)

    As you may kn&<' the~de&tment of &~er& (D&j 1s involved'in'a pronram '. to'chiiracterlze the radjologital cbndif~on of ,sites formerly used byythe . . . ., Manhattan Engineer Dlstrlct (NED) and/or Atomjc Energy Co$n~~lssiqq (AEC); in.. the development of 'nuclear energy.. As part..of this -programi' DOE is 1~ I+ preparing, ,a' series of. brJef~ summaries ,-of .the' history:. of' tho ,#D/AEC~ : : ..; 'i ..relatecl activities and 'Conditions at .thc. sneclfic. sites. The

  14. Synthesis and Application of an Environmentally Insensitive Cy3-Based Arsenical Fluorescent Probe to Identify Adaptive Microbial Responses Involving Proximal Dithiol Oxidation

    SciTech Connect (OSTI)

    Fu, Na; Su, Dian; Cort, John R.; Chen, Baowei; Xiong, Yijia; Qian, Weijun; Konopka, Allan; Bigelow, Diana J.; Squier, Thomas C.

    2013-03-06

    Reversible disulfide oxidation between proximal cysteines in proteins represents a common regulatory control mechanism to modulate flux through metabolic pathways in response to changing environmental conditions. To enable in vivo measurements of cellular redox changes linked to disulfide bond formation, we have synthesized a cell-permeable monosubstituted cyanine dye derivatized with arsenic (i.e., TRAP_Cy3) to trap and visualize dithiols in cytosolic proteins. Alkylation of reactive thiols prior to displacement of the bound TRAP-Cy3 by ethanedithiol permits facile protein capture and mass spectrometric identification of proximal reduced dithiols to the exclusion of individual cysteines. Applying TRAP_Cy3 to evaluate cellular responses to increases in oxygen and light levels in the photosynthetic microbe Synechococcus sp. PCC 7002, we observe large decreases in the abundance of reduced dithiols in cellular proteins, which suggest redox-dependent mechanisms involving the oxidation of proximal disulfides. Under these same growth conditions that result in the oxidation of proximal thiols, there is a reduction in the abundance of post-translational oxidative modifications involving nitrotyrosine and methionine sulfoxide formation. These results suggest that the redox status of proximal cysteines respond to environmental conditions, acting to regulate metabolic flux and minimize the formation of reactive oxygen species to decrease oxidative protein damage.

  15. Kinetics and dynamics of oxidation reactions involving adsorbed CO species on bulk supported Pt and copper oxides. Final project report, January 1, 1991--December 31, 1993

    SciTech Connect (OSTI)

    Conner, Wm.C.; Harold, M.

    1995-02-01

    This research was an integrated experimental and modeling study of oxidation reactions involving CO as a key player - be it a reactant, adsorbed intermediate, and/or partial oxidation product - in the catalytic sequence and chemistry. The reaction systems of interest in the project include CO, formaldehyde, and methanol oxidation by O{sub 2}, and CO oxidation by NO, on both Pt and copper oxide catalysts. These reactions are of importance in automobile exhaust catalysis. There is a paucity of rate data in the literature for these important environmental control reactions. A complicating factor is the propensity of these reactions to exhibit complex steady state and dynamic behavior, including multiple rate controlling steps, steady state multiplicity, and oscillatory phenomena. Such phenomena are rooted in some of the central issues of catalysis, including adsorbate interactions, and catalyst structural instabilities, such as surface reconstruction and surface chemical changes by oxidation- reduction. The goal of this research is to better understand the catalytic chemistry and kinetics of oxidations reactions involving CO as an adsorbed intermediate. Successfully meeting this goal requires an integration of basic kinetic measurements, in situ catalyst surface monitoring, kinetic modeling, and nonlinear mathematical tools. While the kinetics experiments have standard microreactor design, the potential for multiple and periodic rate states demands detailed procedures to pinpoint the bifurcation (ignition, extinction, Hopf) points. Kinetic models are constructed from rational mechanistic sequences and sound surface chemistry.

  16. Microenergetic research involving a coupled experimental and computational approach to evaluate microstructural effects on detonation and combustion at sub-millimeter geometries.

    SciTech Connect (OSTI)

    Nogan, John; Palmer, Jeremy Andrew; Brundage, Aaron L.; Long, Gregory T.; Wroblewski, Brian D.; Tappan, Alexander Smith; Renlund, Anita Mariana; Kravitz, Stanley H.; Baer, Melvin R.

    2006-07-01

    A new approach to explosive sample preparation is described in which microelectronics-related processing techniques are utilized. Fused silica and alumina substrates were prepared utilizing laser machining. Films of PETN were deposited into channels within the substrates by physical vapor deposition. Four distinct explosive behaviors were observed with high-speed framing photography by driving the films with a donor explosive. Initiation at hot spots was directly observed, followed by either energy dissipation leading to failure, or growth to a detonation. Unsteady behavior in velocity and structure was observed as reactive waves failed due to decreasing channel width. Mesoscale simulations were performed to assist in experiment development and understanding. We have demonstrated the ability to pattern these films of explosives and preliminary mesoscale simulations of arrays of voids showed effects dependent on void size and that detonation would not develop with voids below a certain size. Future work involves experimentation on deposited films with regular patterned porosity to elucidate mesoscale explosive behavior.

  17. The UV-absorber benzophenone-4 alters transcripts of genes involved in hormonal pathways in zebrafish (Danio rerio) eleuthero-embryos and adult males

    SciTech Connect (OSTI)

    Zucchi, Sara; Bluethgen, Nancy; Ieronimo, Andrea; Fent, Karl

    2011-01-15

    Benzophenone-4 (BP-4) is frequently used as UV-absorber in cosmetics and materials protection. Despite its frequent detection in the aquatic environment potential effects on aquatic life are unknown. In this study, we evaluate the effects of BP-4 in eleuthero-embryos and in the liver, testis and brain of adult male fish on the transcriptional level by focusing on target genes involved in hormonal pathways to provide a more complete toxicological profile of this important UV-absorber. Eleuthero-embryos and males of zebrafish were exposed up to 3 days after hatching and for 14 days, respectively, to BP-4 concentrations between 30 and 3000 {mu}g/L. In eleuthero-embryos transcripts of vtg1, vtg3, esr1, esr2b, hsd17ss3, cyp19b cyp19a, hhex and pax8 were induced at 3000 {mu}g/L BP-4, which points to a low estrogenic activity and interference with early thyroid development, respectively. In adult males BP-4 displayed multiple effects on gene expression in different tissues. In the liver vtg1, vtg3, esr1 and esr2b were down-regulated, while in the brain, vtg1, vtg3 and cyp19b transcripts were up-regulated. In conclusion, the transcription profile revealed that BP-4 interferes with the expression of genes involved in hormonal pathways and steroidogenesis. The effects of BP-4 differ in life stages and adult tissues and point to an estrogenic activity in eleuthero-embryos and adult brain, and an antiestrogenic activity in the liver. The results indicate that BP-4 interferes with the sex hormone system of fish, which is important for the risk assessment of this UV-absorber.

  18. Low-Dose Involved-Field Radiation in the Treatment of Non-Hodgkin Lymphoma: Predictors of Response and Treatment Failure

    SciTech Connect (OSTI)

    Russo, Andrea L.; Chen, Yu-Hui; Martin, Neil E.; Vinjamoori, Anant; Luthy, Sarah K.; Freedman, Arnold; Michaelson, Evan M.; Silver, Barbara; Mauch, Peter M.; Ng, Andrea K.

    2013-05-01

    Purpose: To investigate clinical and pathologic factors significant in predicting local response and time to further treatment after low-dose involved-field radiation therapy (LD-IFRT) for non-Hodgkin lymphoma (NHL). Methods and Materials: Records of NHL patients treated at a single institution between April 2004 and September 2011 were retrospectively reviewed. Low-dose involved-field radiation therapy was given as 4 Gy in 2 fractions over 2 consecutive days. Treatment response and disease control were determined by radiographic studies and/or physical examination. A generalized estimating equation model was used to assess the effect of tumor and patient characteristics on disease response. A Cox proportional hazards regression model was used to assess time to further treatment. Results: We treated a total of 187 sites in 127 patients with LD-IFRT. Histologies included 66% follicular, 9% chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma, 10% marginal zone, 6% mantle cell lymphoma (MCL), and 8% other. Median follow-up time was 23.4 months (range, 0.03-92.2 months). The complete response, partial response, and overall response rates were 57%, 25%, and 82%, respectively. A CLL histology was associated with a lower response rate (odds ratio 0.2, 95% confidence interval 0.1-0.5, P=.02). Tumor size, site, age at diagnosis, and prior systemic therapy were not associated with response. The median time to first recurrence was 13.6 months. Those with CLL and age ?50 years at diagnosis had a shorter time to further treatment for local failures (hazard ratio [HR] 3.63, P=.01 and HR 5.50, P=.02, respectively). Those with CLL and MCL had a shorter time to further treatment for distant failures (HR 11.1 and 16.3, respectively, P<.0001). Conclusions: High local response rates were achieved with LD-IFRT across most histologies. Chronic lymphocytic leukemia and MCL histologies and age ?50 years at diagnosis had a shorter time to further treatment after LD-IFRT.

  19. Epigenetic involvement of Alien/ESET complex in thyroid hormone-mediated repression of E2F1 gene expression and cell proliferation

    SciTech Connect (OSTI)

    Hong, Wei; College of Basic Medicine, Tianjin Medical University, 300070 Tianjin ; Li, Jinru; Wang, Bo; Chen, Linfeng; Niu, Wenyan; Yao, Zhi; Baniahmad, Aria

    2011-12-02

    Highlights: Black-Right-Pointing-Pointer Corepressor Alien interacts with histone methyltransferase ESET in vivo. Black-Right-Pointing-Pointer Alien/ESET complex is recruited to nTRE of T3-responsive gene by liganded TR{beta}1. Black-Right-Pointing-Pointer ESET-mediated H3K9 methylation is required for liganded TR{beta}1-repressed transcription. Black-Right-Pointing-Pointer ESET is involved in T3-repressed G1/S phase transition and proliferation. -- Abstract: The ligand-bound thyroid hormone receptor (TR) is known to repress via a negative TRE (nTRE) the expression of E2F1, a key transcription factor that controls the G1/S phase transition. Alien has been identified as a novel interacting factor of E2F1 and acts as a corepressor of E2F1. The detailed molecular mechanism by which Alien inhibits E2F1 gene expression remains unclear. Here, we report that the histone H3 lysine 9 (H3K9) methyltransferase (HMT) ESET is an integral component of the corepressor Alien complex and the Alien/ESET complex is recruited to both sites, the E2F1 and the nTRE site of the E2F1 gene while the recruitment to the negative thyroid hormone response element (nTRE) is induced by the ligand-bound TR{beta}1 within the E2F1 gene promoter. We show that, overexpression of ESET promotes, whereas knockdown of ESET releases, the inhibition of TR{beta}1-regulated gene transcription upon T3 stimulation; and H3K9 methylation is required for TR{beta}1-repressed transcription. Furthermore, depletion of ESET impairs thyroid hormone-repressed proliferation as well as the G1/S transition of the cell cycle. Taken together, our data indicate that ESET is involved in TR{beta}1-mediated transcription repression and provide a molecular basis of thyroid hormone-induced repression of proliferation.

  20. Thermal and combined thermal and radiolytic reactions involving nitrous oxide, hydrogen, nitrogen, and ammonia in contact with tank 241-SY-101 simulated waste

    SciTech Connect (OSTI)

    Bryan, S.A.; Pederson, L.R.

    1996-02-01

    Work described in this report was conducted at Pacific Northwest National Laboratory (PNNL) for the Flammable Gas Safety Project, the purpose of which is to develop information needed to support Westinghouse Hanford Company (WHC) in their efforts to ensure the safe interim storage of wastes at the Hanford Site. Described in this report are the results of tests to evaluate the rates of thermal and combined thermal and radiolytic reactions involving flammable gases in the presence of Tank 241-SY-101 simulated waste. Flammable gases generated by the radiolysis of water and by the thermal and radiolytic decomposition of organic waste constituents may themselves participate in further reactions. Examples include the decomposition of nitrous oxide to yield nitrogen and oxygen, the reaction of nitrous oxide and hydrogen to produce nitrogen and water, and the reaction of nitrogen and hydrogen to produce ammonia. The composition of the gases trapped in bubbles in the wastes might therefore change continuously as a function of the time that the gas bubbles are retained.

  1. Analysis of the Salmonella regulatory network suggests involvement of SsrB and H-NS in σE-regulated SPI-2 gene expression

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Jie; Overall, Christopher C.; Nakayasu, Ernesto S.; Kidwai, Afshan S.; Jones, Marcus B.; Johnson, Rudd; Nguyen, Nhu T.; McDermott, Jason E.; Ansong, Charles; Heffron, Fred; et al

    2015-02-10

    The extracytoplasmic functioning sigma factor ?E is known to play an essential role for Salmonella enterica serovar Typhimurium to survive and proliferate in macrophages and mice. However, its regulatory network is not well characterized, especially during infection. Here we used microarray to identify genes regulated by ?E in Salmonella grown in three conditions: a nutrient-rich condition and two others that mimic early and late intracellular infection. We found that in each condition ?E regulated different sets of genes, and notably, several global regulators. When comparing nutrient-rich and infection-like conditions, large changes were observed in the expression of genes involved inmoreSalmonella pathogenesis island (SPI)-1 type-three secretion system (TTSS), SPI-2 TTSS, protein synthesis, and stress responses. In total, the expression of 58% of Salmonella genes was affected by ?E in at least one of the three conditions. An important finding is that ?E up-regulates SPI-2 genes, which are essential for Salmonella intracellular survival, by up-regulating SPI-2 activator ssrB expression at the early stage of infection and down-regulating SPI-2 repressor hns expression at a later stage. Moreover, ?E is capable of countering the silencing of H-NS, releasing the expression of SPI-2 genes. This connection between ?E and SPI-2 genes, combined with the global regulatory effect of ?E, may account for the lethality of rpoE-deficient Salmonella in murine infection.less

  2. t(8; 14) chromosome translocation of the Burkitt lymphoma cell line Daudi occurred during immunoglobulin gene rearrangement and involved the heavy chain diversity region

    SciTech Connect (OSTI)

    Haluska, F.G.; Tsujimoto, Y.; Croce, C.M.

    1987-10-01

    Recent molecular analyses of Burkitt lymphomas carrying the t(8;14) chromosome translocation have indicated that a dichotomy exists regarding the molecular mechanisms by which the translocations occur. Most sporadic Burkitt tumors carry translocations that apparently arise due to mistakes in the immunoglobulin isotype-switching process. In contrast, there is evidence that the translocations of most endemic Burkitt lymphomas occur as a consequence of aberrant V-D-J recombination of variable, diversity, and joining gene segments, catalyzed by the recombinase enzymes. This phenomenon was first noted in follicular lymphomas and chronic lymphocytic leukemias of the B-cell lineage and has been described in T-cell malignancies as well. In each of these cases, analysis of the nucleotide sequence at chromosome breakpoints demonstrated the involvement of immunoglobulin heavy chain J/sub H/ or T-cell-receptor ..cap alpha..-chain J..cap alpha.. gene segments in the translocation. The authors now have cloned and sequenced both the 8q- and 14q+ translocation breakpoints deriving from the t(8;14) translocation of the endemic Burkitt lymphoma line Daudi. The data show that the translocation resulted from a reciprocal exchange between the D/sub H/ region on chromosome 14 and sequences far 5' of the MYC protooncogene on chromosome 8. Features of the nucleotide sequences surrounding the breakpoint further implicate the V-D-J joining machinery in the genesis of chromosome translocation in endemic Burkitt lymphomas and, more generally, in other lymphoid malignancies as well.

  3. GABA/sub B/ receptor activation inhibits Ca/sup 2 +/-activated potassium channels in synaptosomes: involvement of G-proteins

    SciTech Connect (OSTI)

    Ticku, M.K.; Delgado, A.

    1989-01-01

    /sup 86/Rb-efflux assay from preloaded synaptosomes of rat cerebral cortex was developed to study the effect of GABA/sub B/ receptor agonist baclofen on Ca/sup 2 +/-activated K/sup +/-channels. Depolarization of /sup 86/Rb-loaded synaptosomes in physiological buffer increased Ca/sup 2 +/-activated /sup 86/Rb-efflux by 400%. The /sup 86/Rb-efflux was blocked by quinine sulfate, tetraethylammonium, and La/sup 3 +/ indicating the involvement of Ca/sup 2 +/-activated K/sup +/-channels. (-)Baclofen inhibited Ca/sup 2 +/-activated /sup 86/Rb-efflux in a stereospecific manner. The inhibitory effect of (-)baclofen was mediated by GABA/sub B/ receptor activation, since it was blocked by GABA/sub B/ antagonist phaclofen, but not by bicuculline. Further, pertussis toxin also blocked the ability of baclofen or depolarizing action to affect Ca/sup 2 +/-activated K/sup +/-channels. These results suggest that baclofen inhibits Ca/sup 2 +/-activated K/sup +/-channels in synaptosomes and these channels are regulated by G-proteins. This assay may provide an ideal in vitro model to study GABA/sub B/ receptor pharmacology.

  4. Nanowires, Capacitors, and Other Novel Outer-Surface Components Involved in Electron Transfer to Fe(III) Oxides in Geobacter Species

    SciTech Connect (OSTI)

    Lovley, Derek, R.

    2008-12-22

    The overall goal of this project was to better understand the mechanisms by which Geobacter species transfer electrons outside the cell onto Fe(III) oxides. The rationale for this study was that Geobacter species are often the predominant microorganisms involved in in situ uranium bioremediation and the growth and activity of the Geobacter species during bioremediation is primarily supported by electron transfer to Fe(III) oxides. These studies greatly expanded the understanding of electron transfer to Fe(III). Novel concepts developed included the potential role of microbial nanowires for long range electron transfer in Geobacter species and the importance of extracytoplasmic cytochromes functioning as capacitors to permit continued electron transfer during the hunt for Fe(III) oxide. Furthermore, these studies provided target sequences that were then used in other studies to tract the activity of Geobacter species in the subsurface through monitoring the abundance of gene transcripts of the target genes. A brief summary of the major accomplishments of the project is provided.

  5. Cytotoxicity of synthetic cannabinoids on primary neuronal cells of the forebrain: the involvement of cannabinoid CB{sub 1} receptors and apoptotic cell death

    SciTech Connect (OSTI)

    Tomiyama, Ken-ichi; Funada, Masahiko

    2014-01-01

    The abuse of herbal products containing synthetic cannabinoids has become an issue of public concern. The purpose of this paper was to evaluate the acute cytotoxicity of synthetic cannabinoids on mouse brain neuronal cells. Cytotoxicity induced by synthetic cannabinoid (CP-55,940, CP-47,497, CP-47,497-C8, HU-210, JWH-018, JWH-210, AM-2201, and MAM-2201) was examined using forebrain neuronal cultures. These synthetic cannabinoids induced cytotoxicity in the forebrain cultures in a concentration-dependent manner. The cytotoxicity was suppressed by preincubation with the selective CB{sub 1} receptor antagonist AM251, but not with the selective CB{sub 2} receptor antagonist AM630. Furthermore, annexin-V-positive cells were found among the treated forebrain cells. Synthetic cannabinoid treatment induced the activation of caspase-3, and preincubation with a caspase-3 inhibitor significantly suppressed the cytotoxicity. These synthetic cannabinoids induced apoptosis through a caspase-3-dependent mechanism in the forebrain cultures. Our results indicate that the cytotoxicity of synthetic cannabinoids towards primary neuronal cells is mediated by the CB{sub 1} receptor, but not by the CB{sub 2} receptor, and further suggest that caspase cascades may play an important role in the apoptosis induced by these synthetic cannabinoids. In conclusion, excessive synthetic cannabinoid abuse may present a serious acute health concern due to neuronal damage or deficits in the brain. - Highlights: Synthetic cannabinoids (classical cannabinoids, non-classical cannabinoids, and aminoalkylindole derivatives) induce cytotoxicity in mouse forebrain cultures. Synthetic cannabinoid-induced cytotoxicity towards forebrain cultures is mediated by the CB{sub 1} receptor, but not by the CB{sub 2} receptor, and involves caspase-dependent apoptosis. A high concentration of synthetic cannabinoids may be toxic to neuronal cells that express CB{sub 1} receptors.

  6. Additional Survival Benefit of Involved-Lesion Radiation Therapy After R-CHOP Chemotherapy in Limited Stage Diffuse Large B-Cell Lymphoma

    SciTech Connect (OSTI)

    Kwon, Jeanny; Kim, Il Han; Kim, Byoung Hyuck; Kim, Tae Min; Heo, Dae Seog

    2015-05-01

    Purpose: The purpose of this study was to evaluate the role of involved-lesion radiation therapy (ILRT) after rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) chemotherapy in limited stage diffuse large B-cell lymphoma (DLBCL) by comparing outcomes of R-CHOP therapy alone with R-CHOP followed by ILRT. Methods and Materials: We identified 198 patients treated with R-CHOP (median, 6 cycles) for pathologically confirmed DLBCL of limited stage from July 2004 to December 2012. Clinical characteristics of these patients were 33% with stage I and 66.7% with stage II; 79.8% were in the low or low-intermediate risk group; 13.6% had B symptoms; 29.8% had bulky tumors (≥7 cm); and 75.3% underwent ≥6 cycles of R-CHOP therapy. RT was given to 43 patients (21.7%) using ILRT technique, which included the prechemotherapy tumor volume with a median margin of 2 cm (median RT dose: 36 Gy). Results: After a median follow-up of 40 months, 3-year progression-free survival (PFS) and overall survival (OS) were 85.8% and 88.9%, respectively. Multivariate analysis showed ≥6 cycles of R-CHOP (PFS, P=.004; OS, P=.004) and ILRT (PFS, P=.021; OS, P=.014) were favorable prognosticators of PFS and OS. A bulky tumor (P=.027) and response to R-CHOP (P=.012) were also found to be independent factors of OS. In subgroup analysis, the effect of ILRT was prominent in patients with a bulky tumor (PFS, P=.014; OS, P=.030) or an elevated level of serum lactate dehydrogenase (LDH; PFS, P=.004; OS, P=.012). Conclusions: Our results suggest that ILRT after R-CHOP therapy improves PFS and OS in patients with limited stage DLBCL, especially in those with bulky disease or an elevated serum LDH level.

  7. Epithelialmesenchymal transition during oncogenic transformation induced by hexavalent chromium involves reactive oxygen species-dependent mechanism in lung epithelial cells

    SciTech Connect (OSTI)

    Ding, Song-Ze; Yang, Yu-Xiu; Li, Xiu-Ling; Michelli-Rivera, Audrey; Han, Shuang-Yin; Wang, Lei; Pratheeshkumar, Poyil; Wang, Xin; Lu, Jian; Yin, Yuan-Qin; Budhraja, Amit; Hitron, Andrew J.

    2013-05-15

    Hexavalent chromium [Cr(VI)] is an important human carcinogen associated with pulmonary diseases and lung cancer. Exposure to Cr(VI) induces DNA damage, cell morphological change and malignant transformation in human lung epithelial cells. Despite extensive studies, the molecular mechanisms remain elusive, it is also not known if Cr(VI)-induced transformation might accompany with invasive properties to facilitate metastasis. We aimed to study Cr(VI)-induced epithelialmesenchymal transition (EMT) and invasion during oncogenic transformation in lung epithelial cells. The results showed that Cr(VI) at low doses represses E-cadherin mRNA and protein expression, enhances mesenchymal marker vimentin expression and transforms the epithelial cell into fibroblastoid morphology. Cr(VI) also increases cell invasion and promotes colony formation. Further studies indicated that Cr(VI) uses multiple mechanisms to repress E-cadherin expression, including activation of E-cadherin repressors such as Slug, ZEB1, KLF8 and enhancement the binding of HDAC1 in E-cadherin gene promoter, but DNA methylation is not responsible for the loss of E-cadherin. Catalase reduces Cr(VI)-induced E-cadherin and vimentin protein expression, attenuates cell invasion in matrigel and colony formation on soft agar. These results demonstrate that exposure to a common human carcinogen, Cr(VI), induces EMT and invasion during oncogenic transformation in lung epithelial cells and implicate in cancer metastasis and prevention. - Graphical abstract: Epithelialmesenchymal transition during oncogenic transformation induced by hexavalent chromium involves reactive oxygen species-dependent mechanisms in lung epithelial cells. - Highlights: We study if Cr(VI) might induce EMT and invasion in epithelial cells. Cr(VI) induces EMT by altering E-cadherin and vimentin expression. It also increases cell invasion and promotes oncogenic transformation. Catalase reduces Cr(VI)-induced EMT, invasion and transformation.

  8. Analysis of the Salmonella regulatory network suggests involvement of SsrB and H-NS in σE-regulated SPI-2 gene expression

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Jie; Overall, Christopher C.; Nakayasu, Ernesto S.; Kidwai, Afshan S.; Jones, Marcus B.; Johnson, Rudd; Nguyen, Nhu T.; McDermott, Jason E.; Ansong, Charles; Heffron, Fred; et al

    2015-02-10

    The extracytoplasmic functioning sigma factor σE is known to play an essential role for Salmonella enterica serovar Typhimurium to survive and proliferate in macrophages and mice. However, its regulatory network is not well characterized, especially during infection. Here we used microarray to identify genes regulated by σE in Salmonella grown in three conditions: a nutrient-rich condition and two others that mimic early and late intracellular infection. We found that in each condition σE regulated different sets of genes, and notably, several global regulators. When comparing nutrient-rich and infection-like conditions, large changes were observed in the expression of genes involved inmore » Salmonella pathogenesis island (SPI)-1 type-three secretion system (TTSS), SPI-2 TTSS, protein synthesis, and stress responses. In total, the expression of 58% of Salmonella genes was affected by σE in at least one of the three conditions. An important finding is that σE up-regulates SPI-2 genes, which are essential for Salmonella intracellular survival, by up-regulating SPI-2 activator ssrB expression at the early stage of infection and down-regulating SPI-2 repressor hns expression at a later stage. Moreover, σE is capable of countering the silencing of H-NS, releasing the expression of SPI-2 genes. This connection between σE and SPI-2 genes, combined with the global regulatory effect of σE, may account for the lethality of rpoE-deficient Salmonella in murine infection.« less

  9. Breast Cancer Patients With 10 or More Involved Axillary Lymph Nodes Treated by Multimodality Therapy: Influence of Clinical Presentation on Outcome

    SciTech Connect (OSTI)

    Geara, Fady B. . E-mail: fg00@aub.edu.lb; Nasr, Elie; Tucker, Susan L.; Charafeddine, Maya; Dabaja, Bouthaina; Eid, Toufic; Abbas, Jaber; Salem, Ziad; Shamseddine, Ali; Issa, Philip; El Saghir, Nagi

    2007-06-01

    Purpose: To analyze tumor control and survival for breast cancer patients with 10 or more positive lymph nodes without systemic disease, treated by adjuvant radiation alone or combined-modality therapy. Methods and Materials: We reviewed the records of 309 consecutive patients with these characteristics who received locoregional radiotherapy (RT) at our institution. The majority of patients had clinical Stage II or IIIA-B disease (43% and 48%, respectively). The median number of positive axillary lymph nodes was 15 (range, 10-78). Adjuvant therapy consisted of RT alone, with or without chemotherapy, tamoxifen, and/or ovarian castration. Results: The overall 5-year and 10-year disease-free survival (DFS) rates were 20% and 7%, respectively. Median DFS was higher for patients with Stage I-II compared with those with Stage IIIABC (28 vs. 19 months; p = 0.006). Median DFS for patients aged {<=}35 years was lower than that of older patients (12 vs. 24 months; p < 0.0001). Patients treated with a combination therapy had a higher 5-year DFS rate compared with those treated by RT alone (26% vs. 11%; p 0.03). In multivariate analysis, clinical stage (III vs. I, II; relative risk = 1.8, p = 0.002) and age ({<=}35 vs. others; relative risk = 2.6, p <0.001) were found to be independent variables for DFS. Conclusion: This retrospective data analysis identified young age and advanced clinical stage as pertinent and independent clinical prognostic factors for breast cancer patients with advanced axillary disease (10 or more involved nodes). These factors can be used for further prognostic classification.

  10. SU-E-T-587: Monte Carlo Versus Ray-Tracing for Treatment Planning Involving CNS Tumors On the MultiPlan System for CyberKnife Radiosurgery

    SciTech Connect (OSTI)

    Forbang, R Teboh

    2014-06-01

    Purpose: MultiPlan, the treatment planning system for the CyberKnife Robotic Radiosurgery system offers two approaches to dose computation, namely Ray-Tracing (RT), the default technique and Monte Carlo (MC), an option. RT is deterministic, however it accounts for primary heterogeneity only. MC on the other hand has an uncertainty associated with the calculation results. The advantage is that in addition, it accounts for heterogeneity effects on the scattered dose. Not all sites will benefit from MC. The goal of this work was to focus on central nervous system (CNS) tumors and compare dosimetrically, treatment plans computed with RT versus MC. Methods: Treatment plans were computed using both RT and MC for sites covering (a) the brain (b) C-spine (c) upper T-spine (d) lower T-spine (e) L-spine and (f) sacrum. RT was first used to compute clinically valid treatment plans. Then the same treatment parameters, monitor units, beam weights, etc., were used in the MC algorithm to compute the dose distribution. The plans were then compared for tumor coverage to illustrate the difference if any. All MC calculations were performed at a 1% uncertainty. Results: Using the RT technique, the tumor coverage for the brain, C-spine (C3–C7), upper T-spine (T4–T6), lower T-spine (T10), Lspine (L2) and sacrum were 96.8%, 93.1%, 97.2%, 87.3%, 91.1%, and 95.3%. The corresponding tumor coverage based on the MC approach was 98.2%, 95.3%, 87.55%, 88.2%, 92.5%, and 95.3%. It should be noted that the acceptable planning target coverage for our clinical practice is >95%. The coverage can be compromised for spine tumors to spare normal tissues such as the spinal cord. Conclusion: For treatment planning involving the CNS, RT and MC appear to be similar for most sites but for the T-spine area where most of the beams traverse lung tissue. In this case, MC is highly recommended.

  11. Direct Involvement of ombB, omaB and omcB Genes in Extracellular Reduction of Fe(III) by Geobacter sulfurreducens PCA

    SciTech Connect (OSTI)

    Liu, Yimo; Fredrickson, Jim K.; Zachara, John M.; Shi, Liang

    2015-10-01

    The tandem gene clusters orfR-ombB-omaB-omcB and orfS-ombC-omaC-omcC of the metal-reducing bacterium Geobacter sulfurreducens PCA are responsible for trans-outer membrane electron transfer during extracellular reduction of Fe(III)-citrate and ferrihydrite [a poorly crystalline Fe(III) oxide]. Each gene cluster encodes a putative transcriptional factor (OrfR/OrfS), a porin-like outer-membrane protein (OmbB/OmbC), a periplasmic c-type cytochrome (c-Cyt, OmaB/OmaC) and an outer-membrane c-Cyt (OmcB/OmcC). The individual roles of OmbB, OmaB and OmcB in extracellular reduction of Fe(III), however, have remained either uninvestigated or controversial. Here, we showed that replacements of ombB, omaB, omcB and ombB-omaB with an antibiotic gene in the presence of ombC-omaC-omcC had no impact on reduction of Fe(III)-citrate by G. sulfurreducens PCA. Disruption of ombB, omaB, omcB and ombB-omaB in the absence of ombC-omaC-omcC, however, severely impaired the bacterial ability to reduce Fe(III)-citrate as well as ferrihydrite. These results unequivocally demonstrate an overlapping role of ombB-omaB-omcB and ombC-omaC-omcC in extracellular Fe(III) reduction by G. sulfurreducens PCA. Involvement of both ombB-omaB-omcB and ombC-omaC-omcC in extracellular Fe(III) reduction reflects the importance of these trans-outer membrane protein complexes in the physiology of this bacterium. Moreover, the kinetics of Fe(III)-citrate and ferrihydrite reduction by these mutants in the absence of ombC-omaC-omcC were nearly identical, which clearly show that OmbB, OmaB and OmcB contribute equally to extracellular Fe(III) reduction. Finally, orfS was found to have a negative impact on the extracellular reduction of Fe(III)-citrate and ferrihydrite in G. sulfurreducens PCA probably by serving as a transcriptional repressor.

  12. Amended Notice of Intent To Modify the Scope of the EIS and Conduct Additional Public Scoping Meetings, and Notice of Floodplains and Wetlands Involvement for the Northern Pass Project: Federal Register Notice VOlume 78, No. 173- September 6, 2013

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy announces its intent to modify the scope of the Northern Pass Transmission Line Project Environmental Impact Statement (DOE/EIS–0463) and to conduct additional public scoping meetings. Because the proposed Federal action may involve floodplains and wetlands, the draft EIS will include a floodplains and wetlands assessment as appropriate, and the final EIS and/or Record of Decision will include a floodplains and wetlands statement of findings.

  13. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    inquiry-based science activities. Sandia provides all materials and instructions (English and Spanish). For schools outside of our communities, we offer the following...

  14. Community Involvement Fund | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Requests for Proposals are issued at various times throughout the year. Eligible grantees include non-profit community organizations, groups, projects, or coalitions, who want to ...

  15. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Science Bowl is a Jeopardy-like event for high school and middle school students who have a strong interest and academic ability in mathematics and science. The Science Bowl ...

  16. SMR Handbook: Hybrid Energy Systems Involving SMRs

    SciTech Connect (OSTI)

    Shannon M. Bragg-Sitton

    2014-09-01

    Large-scale nuclear reactors are traditionally operated for a singular purpose: steady-state production of dispatchable baseload electricity that is distributed broadly on the electric grid. While this implementation is key to a sustainable, reliable energy grid, SMRs offer new opportunities for increased use of clean nuclear energy for both electric and thermal applications in more locations – while still accommodating the desire to support renewable production sources. This chapter considers a scenario in which renewable generation would be tightly coupled with the nuclear generation source – behind the grid – to meet the grid demand as an integrated energy system while simultaneously producing other commodities with the available thermal energy.

  17. Sandia National Laboratories: About Sandia: Community Involvement:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Education Programs: CroSSlinks Science Volunteers CroSSlinks Science Volunteers The CroSSlinks program, sponsored by Sandia and Lockheed Martin, links the science community with schools to improve science education for Albuquerque students. The program continues Sandia's commitment to the effective utilization of inquiry-based science instruction. Volunteers partner with teachers to advance the teaching of hands-on science in support of district standards and required curriculum. Science

  18. Public Involvement in the Environmental Assessment Process

    Broader source: Energy.gov [DOE]

    A DOE policy regarding the online publication and electronic notification of the availability of draft environmental assessments (EAs) for public comment.

  19. Decom project involves new tools, communication

    SciTech Connect (OSTI)

    1996-05-01

    This article is the May 1996 Power highlights. Included within this section are discussions of the following: (1) Ft. St. Vrain and Yankee Rowe decommissioning, (2) Stuck fuel assembly during refueling operations at PVNGS-2, (3) Restart schedules for Salem-1 & -2 and Indian Point-3, and (4) Reactor vessel annealing demonstration at Marble Hill.

  20. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science, Technology, Engineering, and Math Programs Future scientists photo Our science, technology, engineering, and math (STEM) programs engage students by linking science to the ...

  1. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cyber Technologies Academy CroSSlinks Science Volunteers DOE Regional Science Bowls Family Math Night Family Science Night Science, Technology, Engineering, and Math Programs ...

  2. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Math Night Family Math Night Family Math Night provides an evening of hands-on math activities held at local elementary schools. Students and parents work together on fun math ...

  3. Diet and Cancer Are Cooked Meats Involved

    ScienceCinema (OSTI)

    LLNL - University of California Television

    2009-09-01

    Diet has been associated with differences in cancer rates in human populations for many years. Mark Knize presents the latest research on cancer causes including work performed at Lawrence Livermore National Laboratory investigating some interesting chemical products created when meat is cooked and how to reduce them. Series: Science on Saturday [10/2006] [Health and Medicine] [Science] [Show ID: 11542

  4. TPA_Draft_Public_Involvement_Plan.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  5. UT gets involved at Oak Ridge

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The struggle to maintain the momentum built up winning World War II and to keep the scientific brain trust at the Clinton Laboratories built during that amazing world changing ...

  6. OREM continues involvement with workforce development program

    Broader source: Energy.gov [DOE]

    For the third consecutive year, OREM has partnered with the NNSA to participate in a 10-week workforce development program for college interns.

  7. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Awards reward young people who, through hard work and determination, overcome great personal difficulties to achieve their goals. Their achievements earn them roles as leaders...

  8. Local employee honored for community involvement

    Broader source: Energy.gov [DOE]

    Last month, the East Tennessee Economic Council (ETEC) recognized Colin Colverson, an Energy Department attorney in Oak Ridge, with the prestigious Postma Young Professional Medal. The selection committee chooses an annual recipient for the award that displays elite community service and leadership qualities.

  9. RAPID/Get Involved | Open Energy Information

    Open Energy Info (EERE)

    join us as a contributor or attend one of our upcoming meetings. Be a Contributor Update.jpg RAPID OpenEI Tutorial.pdf Please feel free to log in to OpenEI and make content...

  10. Strategic Public Involvement … Tool Time!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Liz Mattson, Hanford Challenge Engage the public early and often. Educate the public by providing timely, accurate, and understandable information. Incorporate public values in the decision making process and in the decisions made. Prepare future generations for informed engagement and participation. Ice Cream Socials Build a sense of community around Hanford (locally, regionally, and beyond). Ice Cream Socials  Organize Event: › Located in a place people are already gathering › Provide

  11. Department of Energy Idaho - Public Involvement Opportunity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    If you experience problems in reading the files please down load the latest free Adobe ... Environmental Checklist -2 Mb Battelle Energy Alliance, LLC Response to Department of ...

  12. Mound Site Community Involvement Plan 2012

    Office of Legacy Management (LM)

    ... National Oil and Hazardous Substances Pollution Contingency Plan (NCP), codified at ... Universities and other research parks are close by, as well as the Wright Patterson Air ...

  13. Accurate Numerical Simulations Of Chemical Phenomena Involved...

    Office of Scientific and Technical Information (OSTI)

    Authors: Harrison, R.J. ; Vzquez-Mayagoitia, A. ; Hammond, J.R. 1 ; Stony Brook University) 2 + Show Author Affiliations (LCF) LCF ( Publication Date: 2013-09-16 OSTI ...

  14. Public Involvement and Communications Committee - Transcribed...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and implications o Focus on progress o Explain outcomes of CD when available Page 1 Lessons Learned Start with LCD (general public that doesn't know anything about...

  15. Sandia National Laboratories: About Sandia: Community Involvement

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    passionate about volunteering. You'll find Sandians judging science fairs, coaching sports teams, leading scouting troops, sorting food, building houses, and serving on...

  16. Sandia National Laboratories: About Sandia: Community Involvement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    gifts to nearly 500 needy families. A unique giving program at Sandia is our on-site, blood drives. These convenient drives facilitate the donation of over 900 units of blood...

  17. Fukushima Media Involvement: Lessons Learned and Challenges

    SciTech Connect (OSTI)

    Harvey, Geoffrey L.; Koller, Greg L.; Johnson, Wayne L.

    2013-04-01

    Only days after the Fukushima nuclear reactor disaster on March 11, 2011, the Department of Energy’s Pacific Northwest National Laboratory, or PNNL, found itself in a maelstrom of media attention following announcement of the detection of minute levels of radioactive material originating from the damaged reactors 4,500 miles away. Within days, PNNL had set up a technical team in support of the U.S. government’s efforts to assist the devastated country. While a vast amount of information began to flow from Japan, the situation rapidly evolved challenging PNNL scientists and engineers, as well as news media and the general public to deal with a complex and often conflicting information stream. Over the course of about three weeks, PNNL’s News & Media Relations staff and its scientists and engineers responded to more than one hundred requests for information, and engaged in dozens of personal interviews with international, national, regional and local media. While many of the interviews and resulting stories were accurate and well done, to say that all communication went flawless would be far from the truth. In the midst of chaos and confusion, which are part of any significant crisis, hiccoughs are sure to occur. Even when communications guidelines are established and agreed-to ahead of time, and spokespeople promise to stay on message and within their areas of expertise, there is no guarantee the ball will not be dropped on occasion. Addressed here is “the rest of the story.”

  18. Accurate Numerical Simulations Of Chemical Phenomena Involved...

    Office of Scientific and Technical Information (OSTI)

    A paper copy of this document is also available for sale to the public from the National Technical Information Service, Springfield, VA at www.ntis.gov. Authors: Harrison, R.J. ; ...

  19. Arsenite evokes IL-6 secretion, autocrine regulation of STAT3 signaling, and miR-21 expression, processes involved in the EMT and malignant transformation of human bronchial epithelial cells

    SciTech Connect (OSTI)

    Luo, Fei; Xu, Yuan; Ling, Min; Zhao, Yue; Xu, Wenchao; Liang, Xiao; Jiang, Rongrong; Wang, Bairu; Bian, Qian; Liu, Qizhan

    2013-11-15

    Arsenite is an established human carcinogen, and arsenite-induced inflammation contributes to malignant transformation of cells, but the molecular mechanisms by which cancers are produced remain to be established. The present results showed that, evoked by arsenite, secretion of interleukin-6 (IL-6), a pro-inflammatory cytokine, led to the activation of STAT3, a transcription activator, and to increased levels of a microRNA, miR-21. Blocking IL-6 with anti-IL-6 antibody and inhibiting STAT3 activation reduced miR-21 expression. For human bronchial epithelial cells, cultured in the presence of anti-IL-6 antibody for 3 days, the arsenite-induced EMT and malignant transformation were reversed. Thus, IL-6, acting on STAT3 signaling, which up-regulates miR-21in an autocrine manner, contributes to the EMT induced by arsenite. These data define a link from inflammation to EMT in the arsenite-induced malignant transformation of HBE cells. This link, mediated through miRNAs, establishes a mechanism for arsenite-induced lung carcinogenesis. - Highlights: Arsenite evokes IL-6 secretion. IL-6 autocrine mediates STAT3 signaling and up-regulates miR-21expression. Inflammation is involved in arsenite-induced EMT.

  20. H.R. 5299: A Bill to amend the Internal Revenue Code of 1986 to phase out the tax subsidies for alcohol fuels involving alcohol produced from feedstocks eligible to receive Federal agricultural subsidies. Introduced in the House of Representatives, One Hundred Third Congress, Second Session, November 29, 1994

    SciTech Connect (OSTI)

    1994-12-31

    The report H.R. 5299 is a bill to amend the Internal Revenue Code of 1986 to phase out the tax subsidies of alcohol fuels involving alcohol produced from feedstocks eligible to receive Federal agriculture subsidies. The proposed legislative text is included.

  1. Communication methods, systems, apparatus, and devices involving RF tag registration

    DOE Patents [OSTI]

    Burghard, Brion J.; Skorpik, James R.

    2008-04-22

    One technique of the present invention includes a number of Radio Frequency (RF) tags that each have a different identifier. Information is broadcast to the tags from an RF tag interrogator. This information corresponds to a maximum quantity of tag response time slots that are available. This maximum quantity may be less than the total number of tags. The tags each select one of the time slots as a function of the information and a random number provided by each respective tag. The different identifiers are transmitted to the interrogator from at least a subset of the RF tags.

  2. Probative Investigation of the Thermal Stability of Wastes Involved...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This document corresponds to Appendix FG: Reaction Chemistry & HypothesisSummary of Reactivity Testing of the Technical Assessment Team Report. PDF icon Probative Investigation ...

  3. Two concurrent EIS projects involving the same agencies

    SciTech Connect (OSTI)

    Thompson, M.G. ); Schaddel, T.L. . Div. of Aeronautics)

    1993-01-01

    In 1990-91 the US Air Force prepared environmental impact statements (EIS) for the disposal and reuse of Chanute Air Force Base in Rantoul, Illinois and for the joint military-civilian use of Scott Air Force Base in St. Clair County, Illinois. The Federal Aviation Administration, Chicago Airports District Office and the Illinois Department of Transportation, Division of Aeronautics were cooperating agencies for both projects. This paper summarizes the two environmental impact statements and analyzes the processes followed in preparing both documents.

  4. Definite Integrals, Some Involving Residue Theory Evaluated by Maple Code

    SciTech Connect (OSTI)

    Bowman, Kimiko o [ORNL

    2010-01-01

    The calculus of residue is applied to evaluate certain integrals in the range (-{infinity} to {infinity}) using the Maple symbolic code. These integrals are of the form {integral}{sub -{infinity}}{sup {infinity}} cos(x)/[(x{sup 2} + a{sup 2})(x{sup 2} + b{sup 2}) (x{sup 2} + c{sup 2})]dx and similar extensions. The Maple code is also applied to expressions in maximum likelihood estimator moments when sampling from the negative binomial distribution. In general the Maple code approach to the integrals gives correct answers to specified decimal places, but the symbolic result may be extremely long and complex.

  5. Tri-Party Agreement Agencies - Public involvement calendar -...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ...vironmentalAssessments Draft Natural Gas Pipeline and Natural Gas Utility Service ...EnvironmentalImpactStatements Class 3 permit modification to put all SWOC units final ...

  6. Multidomain Carbohydrate-binding Proteins Involved in Bacteroides...

    Office of Scientific and Technical Information (OSTI)

    Starch Metabolism Authors: Cameron, Elizabeth A. ; Maynard, Mallory A. ; Smith, Christopher J. ; Smith, Thomas J. ; Koropatkin, Nicole M. ; Martens, Eric C. 1 ; Danforth) 2 + ...

  7. Weak interaction processes in nuclei involving neutrinos and CDM candidates

    SciTech Connect (OSTI)

    Kosmas, T. S.; Tsakstara, V. [Theoretical Physics Section, University of Ioannina, GR 45110 Ioannina (Greece); Divari, P. C. [Department of Physical Sciences, Hellenic Army Academy, Vari 16673, Attica (Greece); Sinatkas, J. [Department of Informatics and Computer Technology, TEI of Western Macedonia, GR-52100 Kastoria (Greece)

    2009-11-09

    In this work, we concentrate on the nuclear physics aspects of low-energy neutrinos and in particular on problems related to neutrino detection by terrestrial experiments, neutrino astrophysics and neutrino-nucleus interactions. The detection of low-flux neutrinos, feasible by measuring the energy recoil of the recoiling nucleus with gaseous-detectors having very-low threshold-energy, is carried out in conjunction with direct-detection of cold dark matter events and nonstandard physics searches like the neutrinoless double beta decay.

  8. TRI-PARTY AGREEMENT HANFORD PUBLIC INVOLVEMENT PLAN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... of comments is provided in bold italicized text. ... Seattle high school students knowledgeable about ... The U.S. Department of Energy (DOE) and the Washington State ...

  9. Consistent analysis of one-nucleon spectroscopic factors involving...

    Office of Scientific and Technical Information (OSTI)

    Additional Journal Information: Journal Name: Physics Letters. Section B; Journal ID: ISSN 0370-2693 Publisher: Elsevier Sponsoring Org: USDOE Country of Publication: Netherlands ...

  10. GC GUIDANCE ON BARTER TRANSACTIONS INVOLVING DOE-OWNED URANIUM...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    results of analyses previously directed to individual proposed transactions . For the reasons discussed below, we conclude that the Atomic Energy Act of 1954' , as amended, (AEA),...

  11. Overview of Processes Involved in Spin Transfer Collisions

    SciTech Connect (OSTI)

    Buttimore, N. H.

    2008-04-30

    An outline is provided of methods for polarizing antiprotons that include the possible use of channelling in a bent crystal and also the technique of scattering off leptons or protons at a suitably small angle. In the method of channelling it is suggested that the angular dependence of the analysing power of the incident particle is cubic in the angle for single scattering in the crystal when this takes place within the region of electromagnetic hadronic interference as is most likely for channelled particles. Polarization transfer in the scattering of antiprotons off leptons or protons is discussed in addition where emphasis is laid on the angular integrals over spin observables appropriate for spin filtering.

  12. Metaproteomics Identifies the Protein Machinery Involved in Metal...

    Office of Scientific and Technical Information (OSTI)

    This research effort integrated current understanding of the microbiology and biochemistry of U(VI) reduction and capitalize on advances in proteomics technology made over the past ...

  13. OpenEI:Get Involved | Open Energy Information

    Open Energy Info (EERE)

    and contribute to OpenEI. Sign up for an account then start contributing wiki content Use social media to help share the word about OpenEI Join a local energy or technology...

  14. RAPID/Best Practices/Public Involvement | Open Energy Information

    Open Energy Info (EERE)

    - use a broad range of communication tools, including public meetings, a project website, social media accounts, press releases, advertisements in local newspapers and...

  15. Biochemical localization of a protein involved in Gluconacetobacter...

    Office of Scientific and Technical Information (OSTI)

    States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES biofuels (including algae and biomass), bio-inspired, membrane, carbon sequestration, materials and chemistry by...

  16. Metaproteomics Identifies the Protein Machinery Involved in Metal...

    Office of Scientific and Technical Information (OSTI)

    mining, controlled laboratory studies, U(VI) reduction activity measurements, phylogenetic analyses, and gene expression studies to support the metaproteomics characterizations. ...

  17. Creating an Ethical Framework for Studies That Involve the Worker...

    Office of Science (SC) Website

    ... K - Bibliography and Resources .pdf file (26KB) References used in this document are listed and identified, and a list of Internet Web site addresses is also provided. L - ...

  18. Processing and analysis techniques involving in-vessel material generation

    DOE Patents [OSTI]

    Schabron, John F.; Rovani, Jr., Joseph F.

    2011-01-25

    In at least one embodiment, the inventive technology relates to in-vessel generation of a material from a solution of interest as part of a processing and/or analysis operation. Preferred embodiments of the in-vessel material generation (e.g., in-vessel solid material generation) include precipitation; in certain embodiments, analysis and/or processing of the solution of interest may include dissolution of the material, perhaps as part of a successive dissolution protocol using solvents of increasing ability to dissolve. Applications include, but are by no means limited to estimation of a coking onset and solution (e.g., oil) fractionating.

  19. Processing and analysis techniques involving in-vessel material generation

    DOE Patents [OSTI]

    Schabron, John F.; Rovani, Jr., Joseph F.

    2012-09-25

    In at least one embodiment, the inventive technology relates to in-vessel generation of a material from a solution of interest as part of a processing and/or analysis operation. Preferred embodiments of the in-vessel material generation (e.g., in-vessel solid material generation) include precipitation; in certain embodiments, analysis and/or processing of the solution of interest may include dissolution of the material, perhaps as part of a successive dissolution protocol using solvents of increasing ability to dissolve. Applications include, but are by no means limited to estimation of a coking onset and solution (e.g., oil) fractionating.

  20. Worker Involvement Improves Safety at Hanford Site's Plutonium Finishing Plant

    Broader source: Energy.gov [DOE]

    Employees at the Hanford site are working together to find new and innovative ways to stay safe at the Plutonium Finishing Plant, one of the site’s most complex decommissioning projects.

  1. Mutation assays involving blood cells that metabolize toxic substances

    DOE Patents [OSTI]

    Crespi, Charles L.; Thilly, William G.

    1999-01-01

    The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics.

  2. Mutation assays involving blood cells that metabolize toxic substances

    DOE Patents [OSTI]

    Crespi, Charles L.; Thilly, William G.

    1985-01-01

    A line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity) is disclosed. Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. Mutation assays using these cells, and other cells with similar characteristics, are also disclosed.

  3. Mutation assays involving blood cells that metabolize toxic substances

    DOE Patents [OSTI]

    Crespi, C.L.; Thilly, W.G.

    1999-08-10

    The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics. 3 figs.

  4. Kinetics and mechanisms of reactions involving small aromatic reactive intermediates

    SciTech Connect (OSTI)

    Lin, M.C.

    1993-12-01

    Small aromatic radicals such as C{sub 6}H{sub 5}, C{sub 6}H{sub 5}O and C{sub 6}H{sub 4} are key prototype species of their homologs. C{sub 6}H{sub 5} and its oxidation product, C{sub 6}H{sub 5}O are believed to be important intermediates which play a pivotal role in hydrocarbon combustion, particularly with regard to soot formation. Despite their fundamental importance, experimental data on the reaction mechanisms and reactivities of these species are very limited. For C{sub 6}H{sub 5}, most kinetic data except its reactions with NO and NO{sub 2}, were obtained by relative rate measurements. For C{sub 6}H{sub 5}O, the authors have earlier measured its fragmentation reaction producing C{sub 5}H{sub 5} + CO in shock waves. For C{sub 6}H{sub 4}, the only rate constant measured in the gas phase is its recombination rate at room temperature. The authors have proposed to investigate systematically the kinetics and mechanisms of this important class of molecules using two parallel laser diagnostic techniques--laser resonance absorption (LRA) and resonance enhanced multiphoton ionization mass spectrometry (REMPI/MS). In the past two years, study has been focused on the development of a new multipass adsorption technique--the {open_quotes}cavity-ring-down{close_quotes} technique for kinetic applications. The preliminary results of this study appear to be quite good and the sensitivity of the technique is at least comparable to that of the laser-induced fluorescence method.

  5. Public Involvement in the Environmental Assessment Process (DOE, 2010)

    Broader source: Energy.gov [DOE]

    A DOE policy regarding the online publication and electronic notification of the availability of draft environmental assessments (EAs) for public comment.

  6. CHL1 is involved in human breast tumorigenesis and progression

    SciTech Connect (OSTI)

    He, Li-Hong; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Ma, Qin; Shi, Ye-Hui; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Ge, Jie; Zhao, Hong-Meng; Breast Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Li, Shu-Fen; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Tong, Zhong-Sheng; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin

    2013-08-23

    Highlights: CHL1 is down-regulation in breast cancer tissues. Down-regulation of CHL1 is related to high grade. Overexpression of CHL1 inhibits breast cancer cell proliferation and invasion in vitro. CHL1 deficiency induces breast cancer cell proliferation and invasion both in vitro and in vivo. -- Abstract: Neural cell adhesion molecules (CAM) play important roles in the development and regeneration of the nervous system. The L1 family of CAMs is comprised of L1, Close Homolog of L1 (CHL1, L1CAM2), NrCAM, and Neurofascin, which are structurally related trans-membrane proteins in vertebrates. Although the L1CAM has been demonstrated play important role in carcinogenesis and progression, the function of CHL1 in human breast cancer is limited. Here, we found that CHL1 is down-regulated in human breast cancer and related to lower grade. Furthermore, overexpression of CHL1 suppresses proliferation and invasion in MDA-MB-231 cells and knockdown of CHL1 expression results in increased proliferation and invasion in MCF7 cells in vitro. Finally, CHL1 deficiency promotes tumor formation in vivo. Our results may provide a strategy for blocking breast carcinogenesis and progression.

  7. The research performed during the last granting period involved...

    Office of Scientific and Technical Information (OSTI)

    Current Genetics. 55, 273-286. Some of the following papers were listed as "submitted" in the previous renewal: Our major findings are on the last paper which we are still revising ...

  8. Fukushima Media Involvement: Lessons Learned and Challenges - 13261

    SciTech Connect (OSTI)

    Harvey, Geoffrey L.; Johnson, Wayne L.; Koller, Greg L.

    2013-07-01

    Only days after the Fukushima nuclear reactor disaster on March 11, 2011, the DOE's Pacific Northwest National Laboratory, or PNNL, found itself in a maelstrom of media attention following its announcement of the detection of minute levels of radioactive material originating from the damaged reactors 4,500 miles away. Because PNNL develops state-of-the-art ultra-sensitive radionuclide detection and monitoring systems for national security applications, and has some of the equipment operating on its Richland campus, there was little surprise when one of these sophisticated systems led PNNL to be the first to detect measurable radionuclides in the United States. On Wednesday, March 16, 2011, that system detected minuscule levels of short-lived radioactive xenon, a telltale element derived from either weapons testing or a major reactor disruption. Immediately after the detection was announced, a flurry of inquiries nearly overwhelmed staff as governments, scientific organizations, the general public, and reporters struggled to understand and estimate what impacts this disaster might have on health and environment. Over the course of about three weeks, PNNL's News and Media Relations staff and its scientists and engineers responded to more than 100 requests for information, and engaged in dozens of personal interviews with international, national, regional, and local media. While many of the interviews and resulting stories were accurate and well done, not all communication went flawlessly. In the midst of chaos and confusion, which are part of any significant crisis, hiccoughs are sure to occur. Addressed here is 'the rest of the story'. (authors)

  9. Energy conversion system involving change in the density of an upwardly moving liquid

    DOE Patents [OSTI]

    Petrick, Michael (Jolliet, IL)

    1989-01-01

    A system for converting thermal energy into electrical energy includes a fluid reservoir, a relatively high boiling point fluid such as lead or a lead alloy within the reservoir, a downcomer defining a vertical fluid flow path communicating at its upper end with the reservoir and an upcomer defining a further vertical fluid flow path communicating at its upper end with the reservoir. A variable area nozzle of rectangular section may terminate the upper end of the upcomer and the lower end of the of the downcomer communicates with the lower end of the upcomer. A mixing chamber is located at the lower end portion of the upcomer and receives a second relatively low boiling point fluid such as air, the mixing chamber serving to introduce the low boiling point fluid into the upcomer so as to produce bubbles causing the resultant two-phase fluid to move at high velocity up the upcomer. Means are provided for introducing heat into the system preferably between the lower end of the downcomer and the lower end of the upcomer. Power generating means are associated with the one of the vertical fluid flow paths one such power generating means being a magneto hydrodynamic electrical generator.

  10. Phi ({Phi}) and psi ({Psi}) angles involved in malarial peptide bonds determine sterile protective immunity

    SciTech Connect (OSTI)

    Patarroyo, Manuel E.; Moreno-Vranich, Armando; Bermudez, Adriana

    2012-12-07

    Highlights: Black-Right-Pointing-Pointer Phi ({Phi}) and psi ({Psi}) angles determine sterile protective immunity. Black-Right-Pointing-Pointer Modified peptide's tendency to assume a regular conformation related to a PPII{sub L}. Black-Right-Pointing-Pointer Structural modifications in mHABPs induce Ab and protective immunity. Black-Right-Pointing-Pointer mHABP backbone atom's interaction with HLA-DR{beta}1{sup Asterisk-Operator} is stabilised by H-bonds. -- Abstract: Modified HABP (mHABP) regions interacting with HLA-DR{beta}1{sup Asterisk-Operator} molecules have a more restricted conformation and/or sequence than other mHABPs which do not fit perfectly into their peptide binding regions (PBR) and do not induce an acceptable immune response due to the critical role of their {Phi} and {Psi} torsion angles. These angle's critical role was determined in such highly immunogenic, protection-inducing response against experimental malaria using the conformers (mHABPs) obtained by {sup 1}H-NMR and superimposed into HLA-DR{beta}1{sup Asterisk-Operator }-like Aotus monkey molecules; their phi ({Phi}) and psi ({Psi}) angles were measured and the H-bond formation between these molecules was evaluated. The aforementioned mHABP propensity to assume a regular conformation similar to a left-handed polyproline type II helix (PPII{sub L}) led to suggesting that favouring these conformations according to their amino acid sequence would lead to high antibody titre production and sterile protective immunity induction against malaria, thereby adding new principles or rules for vaccine development, malaria being one of them.

  11. Thermal reactive ion etching technique involving use of self-heated cathode

    SciTech Connect (OSTI)

    Yamada, S.; Minami, Y.; Sohgawa, M.; Abe, T.

    2015-04-15

    In this work, the thermal reactive ion etching (TRIE) technique for etching hard-to-etch materials is presented. The TRIE technique employs a self-heated cathode and a thermally insulated aluminum plate is placed on the cathode of a regular reactive ion etching (RIE) system. By optimizing the beam size to support the sample stage, the temperature of the stage can be increased to a desired temperature without a cathode heater. The technique was used to etch a bulk titanium plate. An etch rate of 0.6 μm/min and an etch selectivity to nickel of 100 were achieved with SF{sub 6} plasma. The proposed technique makes a regular RIE system a more powerful etcher without the use of chlorine gas, a cathode heater, and an inductively coupled plasma source.

  12. Heat flux expressions that satisfy the conservation laws in atomistic system involving multibody potentials

    SciTech Connect (OSTI)

    Fu, Yao Song, Jeong-Hoon

    2015-08-01

    Heat flux expressions are derived for multibody potential systems by extending the original Hardy's methodology and modifying Admal & Tadmor's formulas. The continuum thermomechanical quantities obtained from these two approaches are easy to compute from molecular dynamics (MD) results, and have been tested for a constant heat flux model in two distinctive systems: crystalline iron and polyethylene (PE) polymer. The convergence criteria and affecting parameters, i.e. spatial and temporal window size, and specific forms of localization function are found to be different between the two systems. The conservation of mass, momentum, and energy are discussed and validated within this atomistic–continuum bridging.

  13. Investigations of the Fundamental Surface Reactions Involved in the Sorption and Desorption of Radionuclides

    SciTech Connect (OSTI)

    Czerwinski, Ken; Heske, Clemens; Moser, Duane; Misra, Mnoranjan; McMillion, Glen

    2011-04-20

    Models for describing solution- and surface-phase reactions have been used for 30 years, but only recently applicable to complex surfaces. Duff et al., using micro-XANES, found that Pu was concentrated on Mn-oxide and smectite phases of zeolitic tuff, providing an evaluation of contaminant speciation on surfaces for modeling. Experiments at Los Alamos demonstrated that actinides display varying surface residence time distributions, probably reflective of mineral surface heterogeneity. We propose to investigate the sorption/desorption behavior of radionuclides from mineral surfaces, as effected by microorganisms, employing isolates from Nevada Test Site deep alluvium as a model system. Characterizations will include surface area, particle size distribution, x-ray diffraction (XRD), microprobe analysis, extractions, and microbiology. Surface interactions will be assessed by electron spectroscopy (XPS), x-ray absorption fine structure spectroscopy (XAFS), X-ray emission spectroscopy, transmission electron microscopy (TEM) and Scanning electron microscopy (SEM). Desert Research Institute (DRI), University of Nevada, Reno (UNR), and University of Nevada, Las Vegas (UNLV) researchers will collaborate to enhance scientific infrastructure and the understanding of contaminant behavior on surfaces, with broader implications for the management of DOE sites.

  14. Search for doubly charged Higgs bosons with lepton-flavour-violating decays involving tau leptons

    SciTech Connect (OSTI)

    Aaltonen, T.

    2007-12-01

    The authors search for pair production of doubly charged Higgs particles (H{sup {+-}{+-}}) followed by decays into electron-tau (e{tau}) and muon-tau ({mu}{tau}) pairs using a data set corresponding to an integrated luminosity of 350 pb{sup -1} collected from {bar p}p collisions at {radical}s = 1.96 TeV by the CDF II experiment. They search separately for cases where three or four final-state leptons are detected, and then combine the results into limits for each exclusive flavor decay mode of the H{sup {+-}{+-}}. Assuming 100% branching ratios of the H{sup {+-}{+-}} to left-handed e{tau} ({mu}{tau}) pairs, they set an H{sup {+-}{+-}} lower mass limit of 114 (112) GeV/c{sup 2} at the 95% confidence level (C.L.).

  15. Laser-driven deflection arrangements and methods involving charged particle beams

    DOE Patents [OSTI]

    Plettner, Tomas; Byer, Robert L.

    2011-08-09

    Systems, methods, devices and apparatus are implemented for producing controllable charged particle beams. In one implementation, an apparatus provides a deflection force to a charged particle beam. A source produces an electromagnetic wave. A structure, that is substantially transparent to the electromagnetic wave, includes a physical structure having a repeating pattern with a period L and a tilted angle .alpha., relative to a direction of travel of the charged particle beam, the pattern affects the force of the electromagnetic wave upon the charged particle beam. A direction device introduces the electromagnetic wave to the structure to provide a phase-synchronous deflection force to the charged particle beam.

  16. The Bond Between CO and Cp?3U in Cp?3U(CO) involves Backbondingfrom...

    Office of Scientific and Technical Information (OSTI)

    The origin of the large difference between the stretching frequencies in free (2143 cm-1) ... Subject: 37; ADDUCTS; BONDING; ELECTRONS; ORIGIN; URANIUM uranium orbitals backbonding ...

  17. Systematic identification of genes and transduction pathways involved in radio-adaptive response

    SciTech Connect (OSTI)

    Wu, Honglu

    2015-05-22

    Low doses of radiation have been shown to protect against the biological effects of later exposure to toxic levels of radiation. In this study, we propose to identify the molecular mechanisms of this adaptive response by systematically identifying the genes that play a role in radio-protection. In the original proposal, a human cell line that is well-documented to exhibit the radio-adaptive effect was to be used. In this revised study plan, we will use a mouse model, C57BL/6, which has also been well investigated for radio-adaptation. The goal of the proposed study is to enhance our understanding of cellular responses to low doses of radiation exposure at the molecular level.

  18. Compositions and methods involving methyladenosine phosphorylase in the diagnosis and treatment of proliferative disorders

    DOE Patents [OSTI]

    Olopade, Olufunmilayo I.

    2007-03-20

    Disclosed are novel nucleic acid and peptide compositions comprising methylthioadenosine phosphorylase (MTAP) and methods of use for MTAP amino acid sequences and DNA segments comprising MTAP in the diagnosis of human cancers and development of MTAP-specific antibodies. Also disclosed are methods for the diagnosis and treatment of tumors and other proliferative cell disorders, and identification of tumor suppressor genes and gene products from the human 9p21-p22 chromosome region. Such methods are useful in the diagnosis of multiple tumor types such as bladder cancer, lung cancer, breast cancer, pancreatic cancer, brain tumors, lymphomas, gliomas, melanomas, and leukemias.

  19. Effect of hydrotropic salts on phase relationships involving hydrocarbons, water, and alcohols

    SciTech Connect (OSTI)

    Ho, P.C.; Kraus, K.A.

    1980-01-01

    Hydrotropic salts, which can increase the solubility of organic materials in aqueous solutions, are useful to tertiary oil recovery. We have examined effects on solubility of hydrocarbons in water (with and without alcohols) through addition of inorganic hydrotropic salts, such as perchlorates, thiocyanates, and iodides - high in the usual Hofmeister series - and of organic salts such as short chain alkyl benzene sulfonates and other salts based on substituted benzene derivatives. Although the inorganic salts are relatively ineffective in increasing solubility of hydrocarbons in water, many of the organic salts are excellent hydrotropic agents for hydrocarbons. We have examined the phase relationships for several series of aromatic salts such as sulfonates, carboxylates and hydroxycarboxylates, as a function of alkyl-carbon substitution in three-component (hydrocarbon, salt, water) and in four-component (hydrocarbon, salt, alcohol, water) systems. We have also examined miscibility relationships for a given hydrotropic salt as the chain length of alkanes and alkyl benzenes is systematically varied. While miscibilities decrease with increase in chain length of the hydrocarbon, the hydrotropic properties in these systems increase rapidly with the number of alkyl carbons on the benzene ring of the salts and they are relatively insensitive to the type of charged group (sulfonate vs carboxylate) attached to the benzene ring. However, there were significant increases in hydrotropy as one goes from equally substituted sulfonates or carboxylates to salicylates. A number of salts have been identified which have much greater hydrotropic properties for hydrocarbons than such well-known hydrotropic materials as toluene and xylene sulfonates.

  20. Acrolein cytotoxicity in hepatocytes involves endoplasmic reticulum stress, mitochondrial dysfunction and oxidative stress

    SciTech Connect (OSTI)

    Mohammad, Mohammad K.; Alcohol Research Center, University of Louisville ; Avila, Diana; Department of Pharmacology and Toxicology, University of Louisville; Alcohol Research Center, University of Louisville ; Zhang, Jingwen; Alcohol Research Center, University of Louisville ; Barve, Shirish; Department of Pharmacology and Toxicology, University of Louisville; Alcohol Research Center, University of Louisville ; Arteel, Gavin; Alcohol Research Center, University of Louisville ; McClain, Craig; Department of Pharmacology and Toxicology, University of Louisville; Alcohol Research Center, University of Louisville; Robley Rex VAMC, Louisville, KY ; Joshi-Barve, Swati; Department of Pharmacology and Toxicology, University of Louisville; Alcohol Research Center, University of Louisville

    2012-11-15

    Acrolein is a common environmental, food and water pollutant and a major component of cigarette smoke. Also, it is produced endogenously via lipid peroxidation and cellular metabolism of certain amino acids and drugs. Acrolein is cytotoxic to many cell types including hepatocytes; however the mechanisms are not fully understood. We examined the molecular mechanisms underlying acrolein hepatotoxicity in primary human hepatocytes and hepatoma cells. Acrolein, at pathophysiological concentrations, caused a dose-dependent loss of viability of hepatocytes. The death was apoptotic at moderate and necrotic at high concentrations of acrolein. Acrolein exposure rapidly and dramatically decreased intracellular glutathione and overall antioxidant capacity, and activated the stress-signaling MAP-kinases JNK, p42/44 and p38. Our data demonstrate for the first time in human hepatocytes, that acrolein triggered endoplasmic reticulum (ER) stress and activated eIF2?, ATF-3 and -4, and Gadd153/CHOP, resulting in cell death. Notably, the protective/adaptive component of ER stress was not activated, and acrolein failed to up-regulate the protective ER-chaperones, GRP78 and GRP94. Additionally, exposure to acrolein disrupted mitochondrial integrity/function, and led to the release of pro-apoptotic proteins and ATP depletion. Acrolein-induced cell death was attenuated by N-acetyl cysteine, phenyl-butyric acid, and caspase and JNK inhibitors. Our data demonstrate that exposure to acrolein induces a variety of stress responses in hepatocytes, including GSH depletion, oxidative stress, mitochondrial dysfunction and ER stress (without ER-protective responses) which together contribute to acrolein toxicity. Our study defines basic mechanisms underlying liver injury caused by reactive aldehyde pollutants such as acrolein. -- Highlights: ? Human primary hepatocytes and cultured cell lines are used. ? Multiple cell death signaling pathways are activated by acrolein. ? Novel finding of acrolein-induced ER stress ? Acrolein fails to activate ER stress-induced protective responses. ? Combinatorial therapies may be needed for preventing acrolein hepatotoxicity.

  1. PERMITTING OF A PROJECT INVOLVING HYDROGEN: A CODE OFFICIAL’S PERSPECTIVE

    SciTech Connect (OSTI)

    Kallman, Richard A.; Barilo, Nick F.; Murphy, W. F.

    2012-05-11

    Recent growth in the development of hydrogen infrastructure has led to more requests for code officials to approve hydrogen-related projects and facilities. To help expedite the review and approval process, significant efforts have been made to educate code officials on permitting hydrogen vehicle fueling stations and facilities using stationary fuel cells (e.g., backup power for telephone cell tower sites). Despite these efforts, project delays continue because of several factors, including the limited experience of code officials with these types of facilities, submittals that lack the required information (including failure to adequately address local requirements), and submission of poor quality documents. The purpose of this paper is to help project proponents overcome these potential roadblocks and obtain timely approval for a project. A case study of an actual stationary application permitting request is provided to illustrate the value of addressing these issues.

  2. Contract administration involving the remedial investigation and feasibility study at the Feed Materials Production Center

    SciTech Connect (OSTI)

    Not Available

    1991-08-28

    Advanced Sciences, Incorporated (ASI), has been performing a Remedial Investigation and Feasibility Study (RI/FS) at the Feed Materials Production Center (Fernald Facility) at Fernald, Ohio, under an 8 (a) contract with the US Small Business Administration (SBA). The Fernald Facility is a Government-owned facility operated by Westinghouse Materials Company of Ohio (WMCO) under a management and operating contract. The objective of this audit was to evaluate the award and administration of the ASI contract.

  3. Differing Professional Opinions for Technical Issues Involving Environment, Safety and Health

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-07-29

    The Order establishes the DOE Differing Professional Opinion process for employees to raise technical concerns related to environment, safety, and health which cannot be resolved using routine processes. Supersedes DOE P 442.1 and DOE M 442.1-1.

  4. Physical processes involved in strip electrode welding using the method of slatted splicing

    SciTech Connect (OSTI)

    Bushma, V. O. [Moscow State Technological University 'Stankin' (Russian Federation)

    2010-12-15

    Physical processes that take place in a strip electrode during welding using the slatted splicing technique are considered. Flowing of the welding current in the electrode is shown to be the key process which determines electrode heating and melting. Technological receipts are proposed that allow obtaining high-quality welds by the method of slatted splicing.

  5. Global prevalence and distribution of genes and microorganisms involved in mercury methylation

    SciTech Connect (OSTI)

    Podar, Mircea; Gilmour, C C; Brandt, Craig C; Bullock, Allyson L; Brown, Steven D; Crable, Bryan R; Palumbo, Anthony Vito; Somenahally, Anil C; Elias, Dwayne A

    2015-01-01

    Mercury methylation produces the neurotoxic, highly bioaccumulative methylmercury (MeHg). Recent identification of the methylation genes (hgcAB) provides the foundation for broadly evaluating microbial Hg-methylation potential in nature without making explicit rate measurements. We queried hgcAB diversity and distribution in all available microbial metagenomes, encompassing most environments. The genes were found in nearly all anaerobic, but not in aerobic, environments including oxygenated layers of the open ocean. Critically, hgcAB was effectively absent in ~1500 human microbiomes, suggesting a low risk of endogenous MeHg production. New potential methylation habitats were identified, including invertebrate guts, thawing permafrost, coastal dead zones , soils, sediments, and extreme environments, suggesting multiple routes for MeHg entry into food webs. Several new taxonomic groups potentially capable of Hg-methylation emerged, including lineages having no cultured representatives. We begin to address long-standing evolutionary questions about Hg-methylation and ancient carbon fixation mechanisms while generating a new global view of Hg-methylation potential.

  6. High-performance ternary blend polymer solar cells involving both energy transfer and hole relay processes

    SciTech Connect (OSTI)

    Lu, Luyao; Chen, Wei; Xu, Tao; Yu, Luping

    2015-06-04

    The integration of multiple materials with complementary absorptions into a single junction device is regarded as an efficient way to enhance the power conversion efficiency (PCE) of organic solar cells (OSCs). However, because of increased complexity with one more component, only limited high-performance ternary systems have been demonstrated previously. Here we report an efficient ternary blend OSC with a PCE of 9.2%. We show that the third component can reduce surface trap densities in the ternary blend. Detailed studies unravel that the improved performance results from synergistic effects of enlarged open circuit voltage, suppressed trap-assisted recombination, enhanced light absorption, increased hole extraction, efficient energy transfer and better morphology. The working mechanism and high device performance demonstrate new insights and design guidelines for high-performance ternary blend solar cells and suggest that ternary structure is a promising platform to boost the efficiency of OSCs.

  7. Issues Associated with IAEA Involvement in Assured Nuclear Fuel Supply Arrangements

    SciTech Connect (OSTI)

    Kessler, Carol E.; Mathews, Carrie E.

    2008-02-08

    Assured nuclear fuel supply has been discussed at various times as a mechanism to help limit expansion of enrichment and reprocessing (E&R) capability beyond current technology holders. Given the events in the last few years in North Korea and Iran, concern over weapons capabilities gained from acquisition of E&R capabilities has heightened and brought assured nuclear fuel supply (AFS) again to the international agenda. Successful AFS programs can be valuable contributions to strengthening the nonproliferation regime and helping to build public support for expanding nuclear energy.

  8. Discovery of a new ATP-binding motif involved in peptidic azoline...

    Office of Scientific and Technical Information (OSTI)

    OSTI Identifier: 1163365 Resource Type: Journal Article Resource Relation: Journal Name: Nat. Chem. Biol.; Journal Volume: 10; Journal Issue: 10, 2014 Research Org: Advanced Photon ...

  9. Local government involvement in long term resource planning for community energy systems

    SciTech Connect (OSTI)

    Not Available

    1992-03-01

    A program was developed to coordinate governmental, research, utility, and business energy savings efforts, and to evaluate future potential actions, based on actual field data obtained during the implementation of Phase I of the State Resource Plan. This has lead to the establishment of a state conservation and energy efficiency fund for the purpose of establishing a DSM Program. By taking a state wide perspective on resource planning, additional savings, including environmental benefits, can be achieved through further conservation and demand management. This effort has already blossomed into a state directive for DSM programs for the natural gas industry.

  10. Local government involvement in long term resource planning for community energy systems. Demand side management

    SciTech Connect (OSTI)

    Not Available

    1992-03-01

    A program was developed to coordinate governmental, research, utility, and business energy savings efforts, and to evaluate future potential actions, based on actual field data obtained during the implementation of Phase I of the State Resource Plan. This has lead to the establishment of a state conservation and energy efficiency fund for the purpose of establishing a DSM Program. By taking a state wide perspective on resource planning, additional savings, including environmental benefits, can be achieved through further conservation and demand management. This effort has already blossomed into a state directive for DSM programs for the natural gas industry.

  11. Climate Change Scenario Planning in Alaska's National Parks: Stakeholder Involvement in the Decision-Making Process

    SciTech Connect (OSTI)

    Ernst, Kathleen M; Van Riemsdijk, Dr. Micheline

    2013-01-01

    This article studies the participation of stakeholders in climate change decision-making in Alaska s National Parks. We place stakeholder participation within literatures on environmental and climate change decision-making. We conducted participant observation and interviews in two planning workshops to investigate the decision-making process, and our findings are three-fold. First, the inclusion of diverse stakeholders expanded climate change decision-making beyond National Park Service (NPS) institutional constraints. Second, workshops of the Climate Change Scenario Planning Project (CCSPP) enhanced institutional understandings of participants attitudes towards climate change and climate change decision-making. Third, the geographical context of climate change influences the decision-making process. As the first regional approach to climate change decision-making within the NPS, the CCSPP serves as a model for future climate change planning in public land agencies. This study shows how the participation of stakeholders can contribute to robust decisions, may move climate change decision-making beyond institutional barriers, and can provide information about attitudes towards climate change decision-making.

  12. Climate change scenario planning in Alaska's National Parks: Stakeholder involvement in the decision-making process

    SciTech Connect (OSTI)

    Ernst, Kathleen M; Van Riemsdijk, Dr. Micheline

    2013-01-01

    This article studies the participation of stakeholders in climate change decision-making in Alaska s National Parks. We place stakeholder participation within literatures on environmental and climate change decision-making. We conducted participant observation and interviews in two planning workshops to investigate the decision-making process, and our findings are three-fold. First, the inclusion of diverse stakeholders expanded climate change decision-making beyond National Park Service (NPS) institutional constraints. Second, workshops of the Climate Change Scenario Planning Project (CCSPP) enhanced institutional understandings of participants attitudes towards climate change and climate change decision-making. Third, the geographical context of climate change influences the decisionmaking process. As the first regional approach to climate change decision-making within the NPS, the CCSPP serves as a model for future climate change planning in public land agencies. This study shows how the participation of stakeholders can contribute to robust decisions, may move climate change decision-making beyond institutional barriers, and can provide information about attitudes towards climate change decision-making.

  13. Summary of Interim Policy on CERCLA Settlements Involving Municipalities and Municipal Wastes. Fact sheet

    SciTech Connect (OSTI)

    Not Available

    1991-05-01

    The Fact Sheet addresses a consistent agency-wide approach for addressing municipalities and municipal wastes in the Superfund settlement process. The policy also addresses settlements with private generators and transporters of hazardous waste trash derived from a commercial, institutional, or industrial process or activity.

  14. Involvement of the [beta]3-[alpha]3 Loop of the Proline Dehydrogenase...

    Office of Scientific and Technical Information (OSTI)

    OSTI Identifier: 1088585 Resource Type: Journal Article Resource Relation: Journal Name: Biochemistry-US; Journal Volume: 52; Journal Issue: (26) ; 07, 2013 Research Org: Advanced ...

  15. Overview of Scientific Issues Involved in Selection of Polymers for PV Applications: Preprint

    SciTech Connect (OSTI)

    Kempe, M.

    2011-07-01

    Encapsulant materials used in photovoltaic (PV) modules serve multiple purposes. They physically hold components in place, provide electrical insulation, reduce moisture ingress, optically couple superstrate materials (e.g., glass) to PV cells, protect components from mechanical stress by mechanically de-coupling components via strain relief, and protect materials from corrosion. To do this, encapsulants must adhere well to all surfaces, remain compliant, and transmit light after exposure to temperature, humidity, and UV radiation histories. Here, a brief review of some of the polymeric materials under consideration for PV applications is provided, with an explanation of some of their advantages and disadvantages.

  16. E-cadherin junction formation involves an active kinetic nucleation process

    SciTech Connect (OSTI)

    Biswas, Kabir H.; Hartman, Kevin L.; Yu, Cheng -han; Harrison, Oliver J.; Song, Hang; Smith, Adam W.; Huang, William Y. C.; Lin, Wan -Chen; Guo, Zhenhuan; Padmanabhan, Anup; Troyanovsky, Sergey M.; Dustin, Michael L.; Shapiro, Lawrence; Honig, Barry; Zaidel-Bar, Ronen; Groves, Jay T.

    2015-08-19

    Epithelial (E)-cadherin-mediated cell–cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit α-catenin and exhibit remodeled cortical actin. Observations suggest that the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.

  17. Directory of DOE and contractor personnel involved in non-government standards activities

    SciTech Connect (OSTI)

    1995-08-01

    This document contains a listing of DOE employees and DOE contractors who have submitted form DOE F 1300.2, Record of Non-Government Standards Activity. Additional names were added from rosters supplied by non-Government standards bodies.

  18. Phase-Changing Ionic Liquids: CO2 Capture with Ionic Liquids Involving Phase Change

    SciTech Connect (OSTI)

    2010-07-01

    IMPACCT Project: Notre Dame is developing a new CO2 capture process that uses special ionic liquids (ILs) to remove CO2 from the gas exhaust of coal-fired power plants. ILs are salts that are normally liquid at room temperature, but Notre Dame has discovered a new class of ILs that are solid at room temperature and change to liquid when they bind to CO2. Upon heating, the CO2 is released for storage, and the ILs re-solidify and donate some of the heat generated in the process to facilitate further CO2 release. These new ILs can reduce the energy required to capture CO2 from the exhaust stream of a coal-fired power plant when compared to state-ofthe- art technology.

  19. Global prevalence and distribution of genes and microorganisms involved in mercury methylation

    SciTech Connect (OSTI)

    Podar, Mircea; Gilmour, C. C.; Brandt, Craig C.; Soren, Allyson; Brown, Steven D.; Crable, Bryan R.; Palumbo, Anthony Vito; Somenahally, Anil C.; Elias, Dwayne A.

    2015-01-01

    Mercury methylation produces the neurotoxic, highly bioaccumulative methylmercury (MeHg). Recent identification of the methylation genes (hgcAB) provides the foundation for broadly evaluating microbial Hg-methylation potential in nature without making explicit rate measurements. We first queried hgcAB diversity and distribution in all available microbial metagenomes, encompassing most environments. The genes were found in nearly all anaerobic, but not in aerobic, environments including oxygenated layers of the open ocean. Critically, hgcAB was effectively absent in ~1500 human microbiomes, suggesting a low risk of endogenous MeHg production. New potential methylation habitats were identified, including invertebrate guts, thawing permafrost, coastal dead zones, soils, sediments, and extreme environments, suggesting multiple routes for MeHg entry into food webs. Several new taxonomic groups potentially capable of Hg-methylation emerged, including lineages having no cultured representatives. We then begin to address long-standing evolutionary questions about Hg-methylation and ancient carbon fixation mechanisms while generating a new global view of Hg-methylation potential.

  20. Hydrogenolysis of 5-carbon sugars, sugar alcohols, and other methods and compositions for reactions involving hydrogen

    DOE Patents [OSTI]

    Werpy, Todd A [West Richland, WA; Zacher, Alan H [Kennewick, WA

    2002-11-12

    Methods and compositions for reactions of hydrogen over a Re-containing catalyst with compositions containing a 5-carbon sugar, sugar alcohol, or lactic acid are described. It has been surprisingly discovered that reaction with hydrogen over a Re-containing multimetallic catalyst resulted in superior conversion and selectivity to desired products such as propylene glycol. A process for the synthesis of PG from lactate or lactic acid is also described.

  1. Hydrogenolysis Of 5-Carbon Sugars, Sugar Alcohols And Compositions For Reactions Involving Hydrogen

    DOE Patents [OSTI]

    Werpy, Todd A.; Frye, Jr., John G.; Zacher, Alan H.; Miller, Dennis J.

    2004-01-13

    Methods and compositions for reactions of hydrogen over a Re-containing catalyst with compositions containing a 5-carbon sugar, sugar alcohol, or lactic acid are described. It has been surprisingly discovered that reaction with hydrogen over a Re-containing multimetallic catalyst resulted in superior conversion and selectivity to desired products such as propylene glycol. A process for the synthesis of PG from lactate or lactic acid is also described.

  2. Response and Notification Procedures for Data Breaches Involving Personally Identifiable Information

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-10-09

    This Notice concerns actions to address data breaches of personally identifiable information that is collected, processed or maintained by DOE. Extended by DOE N 251.73 until 1-9-09. No cancellation.

  3. Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Edwards, Marcus J.; White, Gaye F.; Norman, Michael; Tome-Fernandez, Alice; Ainsworth, Emma; Shi, Liang; Fredrickson, Jim K.; Zachara, John M.; Butt, Julea N.; Richardson, David J.; et al

    2015-07-01

    Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Å x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX₈C disulfide that, when substituted for AX₈A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation ofmore » a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.« less

  4. E-cadherin junction formation involves an active kinetic nucleation process

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Biswas, Kabir H.; Hartman, Kevin L.; Yu, Cheng -han; Harrison, Oliver J.; Song, Hang; Smith, Adam W.; Huang, William Y. C.; Lin, Wan -Chen; Guo, Zhenhuan; Padmanabhan, Anup; et al

    2015-08-19

    Epithelial (E)-cadherin-mediated cell–cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit α-catenin and exhibit remodeled cortical actin. Observations suggest thatmore » the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.« less

  5. On computing stress in polymer systems involving multi-body potentials from molecular dynamics simulation

    SciTech Connect (OSTI)

    Fu, Yao E-mail: jhsong@cec.sc.edu; Song, Jeong-Hoon E-mail: jhsong@cec.sc.edu

    2014-08-07

    Hardy stress definition has been restricted to pair potentials and embedded-atom method potentials due to the basic assumptions in the derivation of a symmetric microscopic stress tensor. Force decomposition required in the Hardy stress expression becomes obscure for multi-body potentials. In this work, we demonstrate the invariance of the Hardy stress expression for a polymer system modeled with multi-body interatomic potentials including up to four atoms interaction, by applying central force decomposition of the atomic force. The balance of momentum has been demonstrated to be valid theoretically and tested under various numerical simulation conditions. The validity of momentum conservation justifies the extension of Hardy stress expression to multi-body potential systems. Computed Hardy stress has been observed to converge to the virial stress of the system with increasing spatial averaging volume. This work provides a feasible and reliable linkage between the atomistic and continuum scales for multi-body potential systems.

  6. Collective properties of the final state in processes involving the production of hadron jets

    SciTech Connect (OSTI)

    Okorokov, V. A. Ponosov, A. K.

    2013-10-15

    Experimental results obtained by studying soft hadron jets in pion-proton and pion-nucleus reactions at intermediate energies with the aid of traditional collective variables are presented. Analytic approximations that describe, at a qualitative level, the dependences of collective parameters on the energy and multiplicity are proposed. Estimates obtained for strong coupling constant by studying collective variables are in reasonable agreement with its world-average value and with the results extracted by using different methods. The behavior of traditional collective variables as functions of the multiplicity in various interactions makes it possible to obtain a universal estimate for the lower boundary of the region of experimental manifestations of jets in multiparticle-production processes.

  7. Environmental diagnostic analysis of ground water bacteria and their involvement in utilization of aromatic compounds

    SciTech Connect (OSTI)

    Wear, J.E. Jr.

    1993-05-01

    The objective of this study was to examine the hypothesis that select functional groups of bacteria from pristine sites have an innate ability to degrade synthetic aromatics that often contaminate groundwater environments,due to exposure to naturally occurring recalcitrant aromatics in their environment. This study demonstrates that subsurface microbial communities are capable of utilizing lignin and humic acid breakdown products. Utilizers of these compounds were found to be present in most all the wells tested. Even the deepest aquifer tested had utilizers present for all six of the aromatics tested. Highest counts for the aromatics tested were observed with the naturally occurring breakdown products of either lignin or humic acid. Carboxylic acids were found to be an important sole carbon source for groundwater bacteria possibly explained by the fact that they are produced by the oxidative cleavage of aromatic ring structures. The carbohydrate sole carbon sources that demonstrated the greatest densities were ones commonly associated with humics. This study indicates that utilization of naturally occurring aromatic compounds in the subsurface is an important nutritional source for groundwater bacteria. In addition, it suggests that adaptation to naturally occurring recalcitrant substrates is the origin of degradative pathways for xenobiotic compounds with analogous structure. This work has important implications for in situ bioremediation as a method of environmental cleanup.

  8. SO2907, A Putative TonB-dependent Receptor, Is Involved in Dissimilato...

    Office of Scientific and Technical Information (OSTI)

    Many of the biological components that interact with the various iron forms are proposed to be localized in these membrane fractions. To identify the iron-binding proteins acting ...

  9. IDENTIFICATION AND CHARACTERIZATION OF THERMOBIFIDA FUSCA GENES INVOLVED IN PLANT CELL WALL DEGRADATION.

    SciTech Connect (OSTI)

    David B. Wilson

    2006-01-23

    Micro-array experiments identified a number of Thermobifida fusca genes which were upregulated by growth on cellulose or plant biomass. Five of these genes were cloned, overexpressed in E. coli and the expressed proteins were purified and characterized. These were a xyloglucanase,a 1-3,beta glucanase, a family 18 hydrolase and twocellulose binding proteins that contained no catalytic domains. The catalyic domain of the family 74 endoxyloglucanase with a C-terminal, cellulose binding module was crystalized and its 3-dimensional structure was determined by X-ray crystallography.

  10. Structures of the PelD Cyclic Diguanylate Effector Involved in...

    Office of Scientific and Technical Information (OSTI)

    Journal Issue: (36) ; 08, 2013 Publisher: American Society for Biochemistry and Molecular Biology Research Org: Advanced Photon Source (APS), Argonne National Laboratory (ANL), ...

  11. Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.

    SciTech Connect (OSTI)

    Edwards, Marcus J.; White, Gaye F.; Norman, Michael; Tome-Fernandez, Alice; Ainsworth, Emma; Shi, Liang; Fredrickson, Jim K.; Zachara, John M.; Butt, Julea N.; Richardson, David J.; Clarke, Thomas A.

    2015-07-01

    Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Å x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX₈C disulfide that, when substituted for AX₈A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen.

  12. High-performance ternary blend polymer solar cells involving both energy transfer and hole relay processes

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lu, Luyao; Chen, Wei; Xu, Tao; Yu, Luping

    2015-06-04

    The integration of multiple materials with complementary absorptions into a single junction device is regarded as an efficient way to enhance the power conversion efficiency (PCE) of organic solar cells (OSCs). However, because of increased complexity with one more component, only limited high-performance ternary systems have been demonstrated previously. Here we report an efficient ternary blend OSC with a PCE of 9.2%. We show that the third component can reduce surface trap densities in the ternary blend. Detailed studies unravel that the improved performance results from synergistic effects of enlarged open circuit voltage, suppressed trap-assisted recombination, enhanced light absorption, increasedmore » hole extraction, efficient energy transfer and better morphology. The working mechanism and high device performance demonstrate new insights and design guidelines for high-performance ternary blend solar cells and suggest that ternary structure is a promising platform to boost the efficiency of OSCs.« less

  13. This invention relates to methods of generating NP gallium nitride (GaN) across large areas (>1 cm.sup.2) with controlled pore diameters, pore density, and porosity. Also disclosed are methods of generating novel optoelectronic devices based on porous GaN. Additionally a layer transfer scheme to separate and create free-standing crystalline GaN thin layers is disclosed that enables a new device manufacturing paradigm involving substrate recycling. Other disclosed embodiments of this invention relate to fabrication of GaN based nanocrystals and the use of NP GaN electrodes for electrolysis, water splitting, or photosynthetic process applications.

    DOE Patents [OSTI]

    Zhang, Yu; Sun, Qian; Han, Jung

    2015-12-08

    This invention relates to methods of generating NP gallium nitride (GaN) across large areas (>1 cm.sup.2) with controlled pore diameters, pore density, and porosity. Also disclosed are methods of generating novel optoelectronic devices based on porous GaN. Additionally a layer transfer scheme to separate and create free-standing crystalline GaN thin layers is disclosed that enables a new device manufacturing paradigm involving substrate recycling. Other disclosed embodiments of this invention relate to fabrication of GaN based nanocrystals and the use of NP GaN electrodes for electrolysis, water splitting, or photosynthetic process applications.

  14. Regulation of MDA-MB-231 cell proliferation by GSK-3? involves epigenetic modifications under high glucose conditions

    SciTech Connect (OSTI)

    Gupta, Chanchal; Kaur, Jasmine; Tikoo, Kulbhushan

    2014-05-15

    Hyperglycemia is a critical risk factor for development and progression of breast cancer. We have recently reported that high glucose induces phosphorylation of histone H3 at Ser 10 as well as de-phosphorylation of GSK-3? at Ser 9 in MDA-MB-231 cells. Here, we elucidate the mechanism underlying hyperglycemia-induced proliferation in MDA-MB-231 breast cancer cells. We provide evidence that hyperglycemia led to increased DNA methylation and DNMT1 expression in MDA-MB-231 cells. High glucose condition led to significant increase in the expression of PCNA, cyclin D1 and decrease in the expression of PTPN 12, p21 and PTEN. It also induced hypermethylation of DNA at the promoter region of PTPN 12, whereas hypomethylation at Vimentin and Snail. Silencing of GSK-3? by siRNA prevented histone H3 phosphorylation and reduced DNMT1 expression. We show that chromatin obtained after immunoprecipitation with phospho-histone H3 was hypermethylated under high glucose condition, which indicates a cross-talk between DNA methylation and histone H3 phosphorylation. ChIP-qPCR analysis revealed up-regulation of DNMT1 and metastatic genes viz. Vimentin, Snail and MMP-7 by phospho-histone H3, which were down-regulated upon GSK-3? silencing. To the best of our knowledge, this is the first report which shows that interplay between GSK-3? activation, histone H3 phosphorylation and DNA methylation directs proliferation of breast cancer cells. - Highlights: High glucose induces phosphorylation of histone H3 and dephosphorylation of GSK-3?. Moreover, hyperglycemia also leads to increased DNA methylation in MDA-MB-231 cells. Inhibition of GSK-3? prevented histone H3 phosphorylation and reduced DNMT1 levels. Interplay exists between GSK-3?, histone H3 phosphorylation and DNA methylation.

  15. EIS-0431: Amended Notice of Intent to Prepare an Environmental Impact Statement and Notice of Potential Floodplain and Wetland Involvement

    Broader source: Energy.gov [DOE]

    Hydrogen Energy California's Integrated Gasification Combined Cycle and Carbon Capture and Sequestration Project, Kern County, CA

  16. Minimising the error in eigenvalue calculations involving the Boltzmann transport equation using goal-based adaptivity on unstructured meshes

    SciTech Connect (OSTI)

    Goffin, Mark A.; Baker, Christopher M.J.; Buchan, Andrew G.; Pain, Christopher C.; Eaton, Matthew D.; Smith, Paul N.

    2013-06-01

    This article presents a method for goal-based anisotropic adaptive methods for the finite element method applied to the Boltzmann transport equation. The neutron multiplication factor, k{sub eff}, is used as the goal of the adaptive procedure. The anisotropic adaptive algorithm requires error measures for k{sub eff} with directional dependence. General error estimators are derived for any given functional of the flux and applied to k{sub eff} to acquire the driving force for the adaptive procedure. The error estimators require the solution of an appropriately formed dual equation. Forward and dual error indicators are calculated by weighting the Hessian of each solution with the dual and forward residual respectively. The Hessian is used as an approximation of the interpolation error in the solution which gives rise to the directional dependence. The two indicators are combined to form a single error metric that is used to adapt the finite element mesh. The residual is approximated using a novel technique arising from the sub-grid scale finite element discretisation. Two adaptive routes are demonstrated: (i) a single mesh is used to solve all energy groups, and (ii) a different mesh is used to solve each energy group. The second method aims to capture the benefit from representing the flux from each energy group on a specifically optimised mesh. The k{sub eff} goal-based adaptive method was applied to three examples which illustrate the superior accuracy in criticality problems that can be obtained.

  17. Kinetic and Mechanistic Studies of Carbon-to-Metal Hydrogen Atom Transfer Involving Os-Centered Radicals: Evidence for Tunneling

    SciTech Connect (OSTI)

    Lewandowska-Androlojc, Anna; Grills, David C.; Zhang, Jie; Bullock, R. Morris; Miyazawa, Akira; Kawanishi, Yuji; Fujita, Etsuko

    2014-03-05

    We have investigated the kinetics of novel carbon-to-metal hydrogen atom transfer reactions, in which homolytic cleavage of a C-H bond is accomplished by a single metal-centered radical. Studies by means of time-resolved IR spectroscopic measurements revealed efficient hydrogen atom transfer from xanthene, 9,10-dihydroanthracene and 1,4-cyclohexadiene to Cp(CO)2Os and (n5-iPr4C5H)(CO)2Os radicals, formed by photoinduced homolysis of the corresponding osmium dimers. The rate constants for hydrogen abstraction from these hydrocarbons were found to be in the range 1.54 105 M 1 s 1 -1.73 107 M 1 s-1 at 25 C. For the first time, kinetic isotope effects for carbon-to-metal hydrogen atom transfer were determined. Large primary kinetic isotope effects of 13.4 1.0 and 16.6 1.4 were observed for the hydrogen abstraction from xanthene to form Cp(CO)2OsH and (n5-iPr4C5H)(CO)2OsH, respectively, at 25 C. Temperature-dependent measurements of the kinetic isotope effects over a 60 -C temperature range were carried out to obtain the difference in activation energies and the pre-exponential factor ratio. For hydrogen atom transfer from xanthene to (n5-iPr4C5H)(CO)2Os, the (ED - EH) = 3.25 0.20 kcal/mol and AH/AD = 0.056 0.018 values are greater than the semi-classical limits and thus suggest a quantum mechanical tunneling mechanism. The work at BNL was carried out under contract DE-AC02-98CH10886 with the U.S. Department of Energy and supported by its Division of Chemical Sciences, Geosciences & Biosciences, Office of Basic Energy Sciences. RMB also thanks the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences & Biosciences for support. Pacific Northwest National Laboratory is operated by Battelle for the U.S. Department of Energy.

  18. Interleukin 6 promotes endometrial cancer growth through an autocrine feedback loop involving ERKNF-?B signaling pathway

    SciTech Connect (OSTI)

    Che, Qi; Liu, Bin-Ya; Wang, Fang-Yuan; He, Yin-Yan; Lu, Wen; Liao, Yun; Gu, Wei; Wan, Xiao-Ping

    2014-03-28

    Highlights: IL-6 could promote endometrial cancer cells proliferation. IL-6 promotes its own production through an autocrine feedback loop. ERK and NF-?B pathway inhibitors inhibit IL-6 production and tumor growth. IL-6 secretion relies on the activation of ERKNF-?B pathway axis. An orthotopic nude endometrial carcinoma model confirms the effect of IL-6. - Abstract: Interleukin (IL)-6 as an inflammation factor, has been proved to promote cancer proliferation in several human cancers. However, its role in endometrial cancer has not been studied clearly. Previously, we demonstrated that IL-6 promoted endometrial cancer progression through local estrogen biosynthesis. In this study, we proved that IL-6 could directly stimulate endometrial cancer cells proliferation and an autocrine feedback loop increased its production even after the withdrawal of IL-6 from the medium. Next, we analyzed the mechanism underlying IL-6 production in the feedback loop and found that its production and IL-6-stimulated cell proliferation were effectively blocked by pharmacologic inhibitors of nuclear factor-kappa B (NF-?B) and extra-cellular signal-regulated kinase (ERK). Importantly, activation of ERK was upstream of the NF-?B pathways, revealing the hierarchy of this event. Finally, we used an orthotopic nude endometrial carcinoma model to confirm the effects of IL-6 on the tumor progression. Taken together, these data indicate that IL-6 promotes endometrial carcinoma growth through an expanded autocrine regulatory loop and implicate the ERKNF-?B pathway as a critical mediator of IL-6 production, implying IL-6 to be an important therapeutic target in endometrial carcinoma.

  19. Involvement of reactive oxygen species in brominated diphenyl ether-47-induced inflammatory cytokine release from human extravillous trophoblasts in vitro

    SciTech Connect (OSTI)

    Park, Hae-Ryung Kamau, Patricia W.; Loch-Caruso, Rita

    2014-01-15

    Polybrominated diphenyl ethers (PBDEs) are widely used flame retardant compounds. Brominated diphenyl ether (BDE)-47 is one of the most prevalent PBDE congeners found in human breast milk, serum and placenta. Despite the presence of PBDEs in human placenta, effects of PBDEs on placental cell function are poorly understood. The present study investigated BDE-47-induced reactive oxygen species (ROS) formation and its role in BDE-47-stimulated proinflammatory cytokine release in a first trimester human extravillous trophoblast cell line, HTR-8/SVneo. Exposure of HTR-8/SVneo cells for 4 h to 20 ?M BDE-47 increased ROS generation 1.7 fold as measured by the dichlorofluorescein (DCF) assay. Likewise, superoxide anion production increased approximately 5 fold at 10 and 15 ?M and 9 fold at 20 ?M BDE-47 with a 1-h exposure, as measured by cytochrome c reduction. BDE-47 (10, 15 and 20 ?M) decreased the mitochondrial membrane potential by 4764.5% at 4, 8 and 24 h as assessed with the fluorescent probe Rh123. Treatment with 15 and 20 ?M BDE-47 stimulated cellular release and mRNA expression of IL-6 and IL-8 after 12 and 24-h exposures: the greatest increases were a 35-fold increased mRNA expression at 12 h and a 12-fold increased protein concentration at 24 h for IL-6. Antioxidant treatments (deferoxamine mesylate, ()?-tocopherol, or tempol) suppressed BDE-47-stimulated IL-6 release by 54.1%, 56.3% and 37.7%, respectively, implicating a role for ROS in the regulation of inflammatory pathways in HTR-8/SVneo cells. Solvent (DMSO) controls exhibited statistically significantly decreased responses compared with non-treated controls for IL-6 release and IL-8 mRNA expression, but these responses were not consistent across experiments and times. Nonetheless, it is possible that DMSO (used to dissolve BDE-47) may have attenuated the stimulatory actions of BDE-47 on cytokine responses. Because abnormal activation of proinflammatory responses can disrupt trophoblast functions necessary for placental development and successful pregnancy, further investigation is warranted of the impact of ROS and BDE-47 on trophoblast cytokine responses. - Highlights: BDE-47 induced ROS overproduction and mitochondrial dysfunction. BDE-47 stimulated production of proinflammatory cytokines. Antioxidant treatment reduced BDE-47-stimulated ROS generation and cytokine release.

  20. DOE technical standards list: Directory of DOE and contractor personnel involved in non-government standards activities

    SciTech Connect (OSTI)

    1999-05-01

    The body of this document contains a listing of DOE employees and DOE contractors who have submitted form DOE F 1300.2, Record of Non-Government Standards Activity, which is attached to the end of this document. Additional names were added from rosters supplied by non-Government standards bodies. The committees or governing bodies in which the person participates is listed after each name. An asterisk preceding the committee notation indicates that the person has identified himself or herself as the DOE representative on that committee. Appendices to this document are also provided to sort the information by the parent employment organization, by non-Government standards activity, and by the proper names of the non-Government standards organizations and committees. DOE employees and contractors listed in this technical standards list are those recorded as of May 1, 1999.

  1. DOE technical standards list: Directory of DOE and contractor personnel involved in non-government standards activities

    SciTech Connect (OSTI)

    1996-08-01

    The body of this document contains a listing of DOE employees and DOE contractors who have submitted form DOE F 1300.2, Record of Non-Government Standards Activity, which is attached to the end of this document and to DOE Order 1300.2A. Additional names were added from rosters supplied by non-Government standards bodies. The committees or governing bodies in which the person participates is listed after each name. An asterisk preceding the committee notation indicates that the person has identified himself or herself as the DOE representative on that committee. Appendices to this document are also provided to sort the information by the parent employment organization, by non-Government standards activity, and by the proper names of the non-Government standards organizations and committees. DOE employees and contractors listed in this TSL are those recorded as of July 1, 1996.

  2. High Sensitivity Proteomics Assisted Discovery of a Novel Operon Involved in the Assembly of Photosystem II, a Membrane Protein Complex

    SciTech Connect (OSTI)

    Wegener, Kimberly M.; Welsh, Eric A.; Thornton, Leeann E.; Keren, Nir S.; Jacobs, Jon M.; Hixson, Kim K.; Monroe, Matthew E.; Camp, David G.; Smith, Richard D.; Pakrasi, Himadri B.

    2008-10-10

    Photosystem II (PSII) is a large membrane protein complex that performs the water oxidation reactions of the photosynthetic electron transport chain in plants, algae, and cyanobacteria. Utilizing a high-throughput proteomic analysis of isolated PSII complexes from the cyanobacterium Synechocystis sp. PCC 6803, we have identified four PSII associated proteins that are encoded by the cofactor integration operon (cio). This operon contains genes with putative binding domains for chlorophyll, iron-sulfur centers, and bilins. Protein levels of this operon are more abundant in several PSII lumenal mutants, suggesting an accumulation of cio products in partially assembled PSII complexes. This provides a rare example of a bacterial operon whose protein products are translationally coordinated and associated with a single protein complex. Genetic deletion of cio results in decreased oxygen evolution by PSII, suggesting that cio products may function as regulators of PSII complex assembly or degradation, maybe facilitating an uncharacterized step in PSII assembly.

  3. Review of current research and activities involving characterization, abatement, and disposal of lead-containing paint films

    SciTech Connect (OSTI)

    McKnight, M.E.

    1990-05-01

    In response to a recent regulation for abating lead-based paint in housing and other environmental regulations, research projects and other activities are being conducted to provide information on procedures for carrying out abatement and maintenance of lead-containing paint films in a safe and cost-effective manner. Relevant Federal regulations, and current research projects and other activities addressing the issues are reviewed.

  4. Davis-Bacon Act Wage Rates for ARRA-Funded State Energy Program Projects Involving Residential Weatherization Work

    Broader source: Energy.gov [DOE]

    Guidance on using appropriate prevailing wage rates for Davis-Bacon Act (DBA) covered weatherization work performed under the State Energy Program (SEP) using American Recovery and Reinvestment Act of 2009 (ARRA) funds.

  5. Letter: Congratulating Oak Ridge SSAB and its Stewardship Committee for Receiving the EPA's 2006 Citizen's Excellence in Community Involvement Award

    Broader source: Energy.gov [DOE]

    From: Assistant Secretary for Environmental Management, James A. Rispoli To: Mr. Kerry Trammell, Chair Site Specific Advisory Board

  6. Lung cancer-derived Dickkopf1 is associated with bone metastasis and the mechanism involves the inhibition of osteoblast differentiation

    SciTech Connect (OSTI)

    Chu, Tianqing; Teng, Jiajun; Jiang, Liyan; Zhong, Hua; Han, Baohui

    2014-01-17

    Highlights: DKK1 level was associated with NSCLC bone metastases. Lung tumor cells derived DKK1 inhibited osteoblast differentiation. Lung tumor cells derived DKK1 modulates ?-catenin and RUNX2. -- Abstract: Wnt/?-catenin signaling and Dickkopf1 (DKK1) play important roles in the progression of lung cancer, which preferably metastasizes to skeleton. But the role of them in bone dissemination is poorly understood. This study aims to define the role of DKK1 in lung cancer bone metastases and investigate the underlying mechanism. Our results demonstrated that DKK1 over-expression was a frequent event in non-small-cell lung cancer (NSCLC) blood samples, and serous DKK1 level was much higher in bone metastatic NSCLC compared to non-bone metastatic NSCLC. We also found that conditioned medium from DKK1 over-expressing lung cancer cells inhibited the differentiation of osteoblast, determined by alkaline phosphatase activity and osteocalcin secretion, whereas the conditioned medium from DKK1 silencing lung cancer cells exhibited the opposite effects. Mechanistically, DKK1 reduced the level of ?-catenin and RUNX2, as well as inhibiting the nuclear translocation of ?-catenin. Taken together, these results suggested that lung cancer-produced DKK1 may be an important mechanistic link between NSCLC and bone metastases, and targeting DKK1 may be an effective method to treat bone metastase of NSCLC.

  7. EIS-0463: Amended Notice of Intent To Modify the Scope of the EIS and Conduct Additional Public Scoping Meetings, Notice of Floodplains and Wetlands Involvement

    Broader source: Energy.gov [DOE]

    Department of Energy - Presidential Permit Application for Northern Pass Transmission, New Hampshire

  8. On the Theory of Oxidation-Reduction Reactions Involving Electron Transfer. V. Comparison and Properties of Electrochemical and Chemical Rate Constants

    DOE R&D Accomplishments [OSTI]

    Marcus, R. A.

    1962-01-01

    Using a theory of electron transfers which takes cognizance of reorganization of the medium outside the inner coordination shell and of changes of bond lengths inside it, relations between electrochemical and related chemical rate constants are deduced and compared with the experimental data. A correlation is found, without the use of arbitrary parameters. Effects of weak complexes with added electrolytes are included under specified conditions. The deductions offer a way of coordinating a variety of data in the two fields, internally as well as with each those in another. For example, the rate of oxidation or reduction of a series of related reactants by one reagent is correlated with that of another and with that of the corresponding electrochemical oxidation-reduction reaction, under certain specified conditions. These correlations may also provide a test for distinguishing an electron from an atom transfer mechanism. (auth)

  9. Intermolecular interactions involving C-H bonds, 3, Structure and energetics of the interaction between CH{sub 4} and CN{sup {minus}}

    SciTech Connect (OSTI)

    Novoa, J.J.; Whangbo, Myung-Hwan; Williams, J.M.

    1991-12-31

    On the basis of SCF and single reference MP2 calculations, the full potential energy surface of the interaction between CH{sub 4} and CN{sup {minus}} was studied using extended basis sets of up to near Hartree-Fock limit quality. Colinear arrangements C-N{sup {minus}}{hor_ellipsis}H-CH{sub 3} and N-C{sup {minus}}{hor_ellipsis}H-CH{sub 3} are found to be the only two energy minima. The binding energies of these two structures are calculated to be 2.5 and 2.1 kcal/mol, respectively, at the MP2 level. The full vibrational analyses of two structures show a red shift of about 30 cm{sup {minus}1} for the v{sub s} C-H stretching.

  10. Motexafin-Gadolinium and Involved Field Radiation Therapy for Intrinsic Pontine Glioma of Childhood: A Children's Oncology Group Phase 2 Study

    SciTech Connect (OSTI)

    Bradley, Kristin A.; Zhou Tianni; McNall-Knapp, Rene Y.; Jakacki, Regina I.; Pollack, Ian F.

    2013-01-01

    Purpose: To evaluate the effects on 1-year event-free survival (EFS) and overall survival (OS) of combining motexafin and gadolinium (MGd), a potent radiosensitizer, with daily fractionated radiation therapy in children with newly diagnosed intrinsic pontine gliomas. Methods and Materials: Patients with newly diagnosed intrinsic pontine glioma were treated with MGd daily for 5 consecutive days each week, for a total of 30 doses. Patients received a 5- to 10-min intravenous bolus of MGd, 4.4 mg/kg/day, given 2 to 5 h prior to standard dose irradiation. Radiation therapy was administered at a daily dose of 1.8 Gy for 30 treatments over 6 weeks. The total dose was 54 Gy. Results: Sixty eligible children received MGd daily, concurrent with 6 weeks of radiation therapy. The estimated 1-year EFS was 18% {+-} 5%, and the estimated 1-year OS was 53% {+-} 6.5%. The most common grade 3 to 4 toxicities were lymphopenia, transient elevation of liver transaminases, and hypertension. Conclusions: Compared to historical controls, the addition of MGd to a standard 6-week course of radiation did not improve the survival of pediatric patients with newly diagnosed intrinsic pontine gliomas.

  11. Charge-state-resolved ion energy distribution functions of cathodic vacuum arcs: A study involving the plasma potential and biased plasmas

    SciTech Connect (OSTI)

    Anders, Andre; Oks, Efim

    2007-02-15

    Charge-state-resolved ion energy distribution functions were measured for pulsed cathodic arcs taking the sheath into account that formed between the plasma and the entrance of a combined energy and mass spectrometer. An electron emitting probe was employed to independently determine the plasma potential. All results were obtained by averaging over several individual measurements because the instantaneous energy distributions and the plasma potential show large amplitude fluctuations due to the explosive nature of the arc plasma generation. It was found that the ion energy distribution functions in the plasma were independent of the ion charge state. This is in contrast to findings with continuously operating, direct-current arcs that employ a magnetic field at the cathode to steer the cathode spot motion. The different findings indicate the important role of the magnetic steering field for the plasma properties of direct-current arcs. The results are further supported by experiments with 'biased plasmas' obtained by shifting the potential of the anode. Finally, it was shown that the ion energy distributions were broader and shifted to higher energy at the beginning of each arc pulse. The characteristic time for relaxation to steady state distributions is about 100 {mu}s.

  12. Conflict-of-Interest/Non-Disclosure Certificate For Reviewers Involved in the Selection of Subcontractors for NEUP Research and Development Funds

    Broader source: Energy.gov [DOE]

    The Department of Energy has a policy that individuals with a conflict of interest cannot participate in the technical review of procurement proposals. This certification must be completed by...

  13. A Scoping Analysis Of The Impact Of SiC Cladding On Late-Phase Accident Progression Involving CoreConcrete Interaction

    SciTech Connect (OSTI)

    Farmer, M. T.

    2015-11-01

    The overall objective of the current work is to carry out a scoping analysis to determine the impact of ATF on late phase accident progression; in particular, the molten-core concrete interaction portion of the sequence that occurs after the core debris fails the reactor vessel and relocates into containment. This additional study augments previous work by including kinetic effects that govern chemical reaction rates during core-concrete interaction. The specific ATF considered as part of this study is SiC-clad UO2.

  14. Structural and Functional Studies of WlbA: A Dehydrogenase Involved in the Biosynthesis of 2,3-Diacetamido-2,3-dideoxy-d-mannuronic Acid

    SciTech Connect (OSTI)

    Thoden, James B.; Holden, Hazel M.

    2010-09-08

    2,3-Diacetamido-2,3-dideoxy-D-mannuronic acid (ManNAc3NAcA) is an unusual dideoxy sugar first identified nearly 30 years ago in the lipopolysaccharide of Pseudomonas aeruginosa O:3a,d. It has since been observed in other organisms, including Bordetella pertussis, the causative agent of whooping cough. Five enzymes are required for the biosynthesis of UDP-ManNAc3NAcA starting from UDP-N-acetyl-D-glucosamine. Here we describe a structural study of WlbA, the NAD-dependent dehydrogenase that catalyzes the second step in the pathway, namely, the oxidation of the C-3{prime} hydroxyl group on the UDP-linked sugar to a keto moiety and the reduction of NAD{sup +} to NADH. This enzyme has been shown to use {alpha}-ketoglutarate as an oxidant to regenerate the oxidized dinucleotide. For this investigation, three different crystal structures were determined: the enzyme with bound NAD(H), the enzyme in a complex with NAD(H) and {alpha}-ketoglutarate, and the enzyme in a complex with NAD(H) and its substrate (UDP-N-acetyl-D-glucosaminuronic acid). The tetrameric enzyme assumes an unusual quaternary structure with the dinucleotides positioned quite closely to one another. Both {alpha}-ketoglutarate and the UDP-linked sugar bind in the WlbA active site with their carbon atoms (C-2 and C-3{prime}, respectively) abutting the re face of the cofactor. They are positioned {approx}3 {angstrom} from the nicotinamide C-4. The UDP-linked sugar substrate adopts a highly unusual curved conformation when bound in the WlbA active site cleft. Lys 101 and His 185 most likely play key roles in catalysis.

  15. Gastrodin stimulates anticancer immune response and represses transplanted H22 hepatic ascitic tumor cell growth: Involvement of NF-?B signaling activation in CD4 + T cells

    SciTech Connect (OSTI)

    Shu, Guangwen; Yang, Tianming; Wang, Chaoyuan; Su, Hanwen; Xiang, Meixian

    2013-06-15

    Gastrodia elata Blume (G. elata) is a famous restorative food in East Asia. It can be used as an auxiliary reagent in hepatocellular carcinoma (HCC) treatment. Previous studies unveiled that G. elata exhibited immunomodulatory activities. To explore the active ingredients contributing to its immunomodulatory activities, gastrodin, vanillin, and parishin B were purified from G. elata and their anti-HCC effects were assessed in vivo. Among these compounds, only gastrodin was capable of repressing transplanted H22 ascitic hepatic tumor cell growth in vivo with low toxicity. Further investigations were designed to explore the effects of gastrodin on the immune system of tumor-bearing mice and potential molecular mechanisms underlying these effects. Our data showed that gastrodin ameliorated tumor cell transplantation-induced activation of endogenous pro-apoptotic pathway in CD4 + T cells and abnormalities in serum cytokine profiles in host animals. These events enhanced cytotoxic activities of natural killer and CD8 + T cells against H22 hepatic cancer cells. Gastrodin administration specifically upregulated mRNA levels of several nuclear factor ?B (NF-?B) responsive genes in CD4 + T cells but not in CD8 + T cells. Chromatin immunoprecipitation assay showed that gastrodin increased the association of NF-?B p65 subunit to the promoter regions of IL-2 and Bcl-2 encoding genes in CD4 + T cells. Our investigations demonstrated that gastrodin is the main active ingredient contributing to the anticancer immunomodulatory properties of G. elata. Promoting NF-?B-mediated gene transcription in CD4 + T cells is implicated in its immunomodulatory activity. - Highlights: Gastrodin stimulates anticancer immune response. Gastrodin represses tumor transplantation-induced CD4 + T cell apoptosis. Gastrodin activates NF-?B activity in CD4 + T cells.

  16. Analysis of mutational signatures in exomes from B-cell lymphoma cell lines suggest APOBEC3 family members to be involved in the pathogenesis of primary effusion lymphoma

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wagener, R.; Alexandrov, L. B.; Montesinos-Rongen, M.; Schlesner, M.; Haake, A.; Drexler, H. G.; Richter, J.; Bignell, G. R.; McDermott, U.; Siebert, R.

    2015-02-04

    Here, primary effusion lymphoma (PEL) is a rare large B-cell neoplasm particularly affecting immunodeficient hosts with an increased incidence in young or middle-aged males infected with the HIV.1 The clinical outcome of patients with PEL is unfavorable with a median survival of <6 months.1 PEL has been closely associated with human herpes virus 8 (HHV8, previously called Kaposi sarcoma herpesvirus) infection.1 In some cases a coinfection of HHV8 with the Epstein–Barr Virus (EBV) has been described.1 HHV8 encodes various genes homologous to cellular genes that have proliferative and anti-apoptotic functions.2 Although HHV8 is supposed to be a major driver ofmore » PEL, it alone is not sufficient for a full-blown lymphomagenesis.2 PEL usually shows complex karyotypes with many chromosomal aberrations.3 This chromosomal complexity might be driven by the viral infection and lead to genetic alterations cooperating with HHV8 in PEL lymphomagenesis.4« less

  17. The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: Involvement of interleukin-2 system

    SciTech Connect (OSTI)

    Cervia, Davide; Department of Biomedical and Clinical Sciences, Luigi Sacco University Hospital, University of Milan, Milano ; Catalani, Elisabetta; Belardinelli, Maria Cristina; Perrotta, Cristiana; Picchietti, Simona; Alimenti, Claudio; Casini, Giovanni; Fausto, Anna Maria; Vallesi, Adriana

    2013-02-01

    Water-soluble protein signals (pheromones) of the ciliate Euplotes have been supposed to be functional precursors of growth factors and cytokines that regulate cellcell interaction in multi-cellular eukaryotes. This work provides evidence that native preparations of the Euplotes raikovi pheromone Er-1 (a helical protein of 40 amino acids) specifically increases viability, DNA synthesis, proliferation, and the production of interferon-?, tumor necrosis factor-?, interleukin (IL)-1?, IL-2, and IL-13 in human Jurkat T-cells. Also, Er-1 significantly decreases the mRNA levels of the ? and ? subunits of IL-2 receptor (IL-2R), while the mRNA levels of the ? subunit appeared to be not affected. Jurkat T-cell treatments with Er-1 induced the down-regulation of the IL-2R? subunit by a reversible and time-dependent endocytosis, and increased the levels of phosphorylation of the extracellular signal-regulated kinases (ERK). The cell-type specificity of these effects was supported by the finding that Er-1, although unable to directly influence the growth of human glioma U-373 cells, induced Jurkat cells to synthesize and release factors that, in turn, inhibited the U-373 cell proliferation. Overall, these findings imply that Er-1 coupling to IL-2R and ERK immuno-enhances T-cell activity, and that this effect likely translates to an inhibition of glioma cell growth. -- Highlights: ? Euplotes pheromone Er-1 increases the growth of human Jurkat T-cells. ? Er-1 increases the T-cell production of specific cytokines. ? Er-1 activates interleukin-2 receptor and extracellular signal-regulated kinases. ? The immuno-enhancing effect of Er-1 on Jurkat cells translates to an inhibition of human glioma cell growth.

  18. ROS and NF-{kappa}B are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes

    SciTech Connect (OSTI)

    Ye Shefang Wu Yihui; Hou Zhenqing; Zhang Qiqing

    2009-02-06

    Carbon nanotubes (CNTs) have potential applications in biosensors, tissue engineering, and biomedical devices because of their unique physico-chemical, electronic and mechanical properties. However, there is limited literature data available concerning the biological properties and toxicity of CNTs. This study aimed to assess the toxicity exhibited by multi-walled CNTs (MWCNTs) and to elucidate possible molecular mechanisms underlying the biological effects of MWCNTs in A549 cells. Exposing A549 cells to MWCNTs led to cell death, changes in cell size and complexity, reactive oxygen species (ROS) production, interleukin-8 (IL-8) gene expression and nuclear factor (NF)-{kappa}B activation. Treatment of A549 cells with antioxidants prior to adding MWCNTs decreased ROS production and abrogated expression of IL-8 mRNA. Pretreatment of A549 cells with NF-{kappa}B inhibitors suppressed MWCNTs-induced IL-8 mRNA expression. These results indicate that MWCNTs are able to induce expression of IL-8 in A549 cells, at least in part, mediated by oxidative stress and NF-{kappa}B activation.

  19. Type B Accident Investigation Board Report on the February 27, 1998, Shipping Violations Involving the Corehole 8 Project at the Oak Ridge National Laboratory, Oak Ridge, Tennesee

    Broader source: Energy.gov [DOE]

    This report is an independent product of the Type B Investigation Board appointed by James C. Hall, Manager, Oak Ridge Operations Office, U.S. Department of Energy. The Board was appointed to perform a Type B investigation of these incidents and to prepare an investigation report in accordance with DOE Order 225.1A, Accident Investigations.

  20. Natural indoles, indole-3-carbinol and 3,3′-diindolymethane, inhibit T cell activation by staphylococcal enterotoxin B through epigenetic regulation involving HDAC expression

    SciTech Connect (OSTI)

    Busbee, Philip B.; Nagarkatti, Mitzi; Nagarkatti, Prakash S.

    2014-01-01

    Staphylococcal enterotoxin B (SEB) is a potent exotoxin produced by the Staphylococcus aureus. This toxin is classified as a superantigen because of its ability to directly bind with MHC-II class molecules followed by activation of a large proportion of T cells bearing specific Vβ-T cell receptors. Commonly associated with classic food poisoning, SEB has also been shown to induce toxic shock syndrome, and is also considered to be a potential biological warfare agent because it is easily aerosolized. In the present study, we assessed the ability of indole-3-carbinol (I3C) and one of its byproducts, 3,3′-diindolylmethane (DIM), found in cruciferous vegetables, to counteract the effects of SEB-induced activation of T cells in mice. Both I3C and DIM were found to decrease the activation, proliferation, and cytokine production by SEB-activated Vβ8{sup +} T cells in vitro and in vivo. Interestingly, inhibitors of histone deacetylase class I (HDAC-I), but not class II (HDAC-II), showed significant decrease in SEB-induced T cell activation and cytokine production, thereby suggesting that epigenetic modulation plays a critical role in the regulation of SEB-induced inflammation. In addition, I3C and DIM caused a decrease in HDAC-I but not HDAC-II in SEB-activated T cells, thereby suggesting that I3C and DIM may inhibit SEB-mediated T cell activation by acting as HDAC-I inhibitors. These studies not only suggest for the first time that plant-derived indoles are potent suppressors of SEB-induced T cell activation and cytokine storm but also that they may mediate these effects by acting as HDAC inhibitors. - Highlights: • I3C and DIM reduce SEB-induced T cell activation and inflammatory cytokines. • Inhibiting class I HDACs reduces T cell activation and inflammatory cytokines. • Inhibiting class II HDACs increases T cell activation and inflammatory cytokines. • I3C and DIM selectively reduce mRNA expression of class I HDACs. • Novel use and mechanism to counteract SEB with I3C and DIM.

  1. One of the Largest Pieces of Processing Equipment Removed from Plutonium Finishing Plant- Worker involvement led to safe completion of high-hazard work

    Broader source: Energy.gov [DOE]

    RICHLAND, WASH. – U.S. Department of Energy (DOE) contractor CH2M HILL Plateau Remediation Company (CH2M HILL) announced today the successful removal of one of the largest, most complex pieces of equipment from the Plutonium Finishing Plant (PFP) at the Hanford Site in southeast Washington State.

  2. EIS-0389: Notice of Intent To Prepare an Environmental Impact Statement and Conduct Scoping Meetings; Notice of Floodplain and Wetlands Involvement

    Broader source: Energy.gov [DOE]

    Construction and Operation of the Trinity Public Utility District Direct Interconnection Project, Trinity County, California

  3. EIS-0429: Amended Notice of Intent To Prepare the Environmental Impact Statement; Conduct Additional Public Scoping Meetings; and Issue a Notice of Floodplains and Wetlands Involvement

    Broader source: Energy.gov [DOE]

    Department of Energy Proposed Federal Loan Guarantee for the Indiana Gasification, LLC, Industrial Gasification Facility in Rockport, IN, and CO2 Pipeline

  4. Thermal and combined thermal and radiolytic reactions involving nitrous oxide, hydrogen, and nitrogen in the gas phase; comparison of gas generation rates in supernate and solid fractions of Tank 241-SY-101 simulated waste

    SciTech Connect (OSTI)

    Bryan, S.A.; Pederson, L.R.

    1995-03-01

    This report summarizes progress made in evaluating me by which flammable gases are generated in Hanford double-shell tank wastes, based on the results of laboratory tests using simulated waste mixtures. Work described in this report. was conducted at Pacific Northwest Laboratory (PNL) for the Flammable Gas Safety Project, the purpose of which is to develop information needed to support Westinghouse Hanford Company (WHC) in their efforts to ensure the safe interim storage of wastes at the Hanford Site. This work is related to gas generation studies being performed at Georgia Institute of Technology (GIT), under subcontract to PNL, using simulated wastes, and to studies being performed at VMC using actual wastes.

  5. Notice of Intent To Prepare an Environmental Impact Statement and Notice of Proposed Floodplain and Wetlands Involvement for the Mesaba Energy Project Integrated Gasification Combined Cycle (IGCC) Demonstration Plant Northern Minnesota Iron Range

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Notice of Availability DOE/EA-1906: The Final EA for the Operations, Upgrades, and Consolidation at the Western Command site, KAFB, New Mexico (DOE/EA-1906) is available in hard copy at the Zimmerman Library UNM Campus, Albuquerque, NM and the KAFB Library, Bldg 20204, Kirtland AFB NM; electronically on this web page under NNSA Headquarters/ Office of Secure Transportation and on the DOE web site, www.energy.gov/NEPA. DOE/EA-1929: The Finding of No Significant Impact and the Final Environmental

  6. Applying Section 404(r) of the Clean Water Act to Federal Projects Which Involve the Discharge of Dredged or Fill Materials into Waters of the U.S., Including Wetlands (CEQ, 1980)

    Broader source: Energy.gov [DOE]

    This Council on Environmental Quality memorandum establishes procedures for coordinating agency views and formulating Administration policy prior to requesting Congressional action on projects that may be subject to Section 404(r) of the Clean Water Act (Federal Water Pollution Control Act, as amended).

  7. Development of a Chemoenzymatic-like and Photoswitchable Method for the High-Throughput creation of Protein Microarrays. Application to the Analysis of the Protein/Protein Interactions Involved in the YOP Virulon from Yersinia pestis.

    SciTech Connect (OSTI)

    Camarero, J A

    2006-12-07

    Protein arrays are ideal tools for the rapid analysis of whole proteomes as well as for the development of reliable and cheap biosensors. The objective of this proposal is to develop a new ligand assisted ligation method based in the naturally occurring protein trans-splicing process. This method has been used for the generation of spatially addressable arrays of multiple protein components by standard micro-lithographic techniques. Key to our approach is the use of the protein trans-splicing process. This naturally occurring process allows the development of a truly generic and highly efficient method for the covalent attachment of proteins through its C-terminus to any solid support. This technology has been used for the creation of protein chips containing several virulence factors from the human pathogen Y. pestis.

  8. Notice of Intent to Prepare an Environmental Impact Statement and to Conduct Public Scoping Meetings, and Notice of Floodplain and Wetlands Involvement for Remediation of the Moab Uranium Mill Tailings Site in Grand County, UT (12/20/02)

    Office of Environmental Management (EM)

    Approach | Department of Energy Change in National Environmental Policy (NEPA) Compliance Approach Notice of Change in National Environmental Policy (NEPA) Compliance Approach Depleted Uranium Hexafluoride (DUF6) Conversion Facilities Project Notice of Change in National Environmental Policy (NEPA) Compliance Approach for the Depleted Uranium Hexafluoride (DUF6) Conversion Facilities Project (4/28/03). The purpose of this Notice is to inform the public of the change in the approach for the

  9. DOE O 442.2 Differing Professional Opinions for Technical Issues Involving Environment, Safety and Health DOE O 470.4B Safeguards and Security Program DOE O 472.2 Personnel Security

    Broader source: Energy.gov [DOE]

    The Department issued Contractor Requirements Documents (CRDs) for the subject Directives as follows: DOE O 442.2 (July 29, 2011), DOE O 470.4B (July 21, 2011) and DOE O 472.2 (July 27, 2011).

  10. Structure and sequence analyses of Bacteroides proteins BVU_4064 and BF1687 reveal presence of two novel predominantly-beta domains, predicted to be involved in lipid and cell surface interactions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Natarajan, Padmaja; Punta, Marco; Kumar, Abhinav; Yeh, Andrew P; Godzik, Adam; Aravind, L.

    2015-01-16

    N-terminal domains of BVU_4064 and BF1687 proteins from Bacteroides vulgatus and Bacteroides fragilis respectively are members of the Pfam family PF12985 (DUF3869). Proteins containing a domain from this family can be found in most Bacteroides species and, in large numbers, in all human gut microbiome samples. Both BVU_4064 and BF1687 proteins have a consensus lipobox motif implying they are anchored to the membrane, but their functions are otherwise unknown. The C-terminal half of BVU_4064 is assigned to protein family PF12986 (DUF3870); the equivalent part of BF1687 was unclassified.

  11. Physics of Electronic and Atomic Collisions: XXI International Conference, AIP Conference Proceedings, No. 500 [APCPCS

    SciTech Connect (OSTI)

    Itikawa, Y.; Okuno, K.; Tanaka, H.; Yagishita, A.; Matsuzawa, M.

    2000-12-31

    Topics covered in this conference included collisions involving photons, collisions involving electrons and positrons, and collisions involving heavy projectiles.

  12. Slide 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Public Involvement & Communication Committee Meeting Nov. 4

  13. Integrated Analysis of Protein Complexes and Regulatory Networks...

    Office of Scientific and Technical Information (OSTI)

    and Regulatory Networks Involved in Anaerobic Energy Metabolism of Shewanella ... and Regulatory Networks Involved in Anaerobic Energy Metabolism of Shewanella ...

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  16. American LNG Hialeah Facility Terminal

    Broader source: Energy.gov [DOE]

    1. R = Registration of company; C (LNG) = Contract involving LNG; C (NG)= Contract involving natural gas supply

  17. Final Proposal

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  1. BPA Tribal Policy

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  7. Columbia River Treaty

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  8. Doing Business

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    Finance & Rates Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business Skip navigation links Bonneville Purchasing Instructions Buying...

  9. Final Meeting Summary Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    December 10, 2013 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT COMMITEE December 10, 2013 Richland, WA Topics in this Meeting Summary Welcome ....................................................................................................................................................... 1 Strategic Public Involvement - Tool Time! .................................................................................................. 3 100-F Area Public Involvement

  10. Colorado STEP Training

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    professionals what to do should they arrive at an accident involving a WIPP shipment. ... the unlikely event of a transportation accident involving a shipment headed for WIPP. ...

  11. Bharat Petroleum Corporation Ltd BPCL | Open Energy Information

    Open Energy Info (EERE)

    Mumbai-based company, majority owned by the government and involved in the storing, marketing and distribution of petroleum products. Also involved with the development of wind...

  12. Completion of BPAT Systems Update - October 16, 2014

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  14. Dynamic Transfer Limits Study

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  15. Enhanced BPA Balancing Authority (EBBA)

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  17. Intra-Hour Scheduling

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  18. Previous Agreements

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  19. Email List

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  20. Operational Controls for Balancing Reserves

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  1. Retrospective Reports 2011

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  2. Spring Operations Call 2012

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  3. Retrospective Reports 2012

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  4. Projects & Initiatives

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  5. Spring Operations Call 2013

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  6. BPAT Systems Reminder - October 27, 2015

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    Reminder---October-27,-2015 Sign In About | Careers | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement...

  7. BPAT Systems Update - October 23, 2015

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  8. Home

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    ATC Methodology Pages default Sign In About | Careers | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement...

  9. Hydropower and the Environment - Energy Explained, Your Guide...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...PublicInvolvementCommunityEducationValueoftheRiverPagesHydropower.aspx Hydropower Video - http:www.bpa.govPublicInvolvementCommunityEducationValueoftheRiverPages...

  10. Definitions - WXYZ

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    Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business Skip navigation links Newsroom About Us Civil Rights - EEO Freedom of Information...

  11. Definitions---M2

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    Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business Skip navigation links Newsroom About Us Civil Rights - EEO Freedom of Information...

  12. Audits

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    Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business Skip navigation links Newsroom About Us Civil Rights - EEO Freedom of Information...

  13. Definitions - TUV

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    Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business Skip navigation links Newsroom About Us Civil Rights - EEO Freedom of Information...

  14. Definitions - PQ

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    Expand Finance & Rates Involvement & Outreach Expand Involvement & Outreach Doing Business Expand Doing Business Skip navigation links Newsroom About Us Civil Rights - EEO...

  15. Department of Energy Honors Hanford Site Contractor for Employee...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Site Contractor for Employee Involvement and Management Leadership in Safety and Health Department of Energy Honors Hanford Site Contractor for Employee Involvement and...

  16. Proposals

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    Proposals Sign In About | Careers | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools...

  17. Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Board River and Plateau (RAP) Meeting Public Involvement Meetings Hanford Advisory Board Health, Safety, and Environmental Protection (HSEP) Committee Meeting Public Involvement...

  18. Employee-Led Safety Committees

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    includes both government and private business 6 Getting Employees Involved Involvement ... Culture Change * "Culture change" to organization - Often first time to share safety ...

  19. Customer Comments

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acscustomercomments Sign In About | Careers | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports &...

  20. Customer Comments

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    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  1. file://L:\\DOE-hanford.gov\\public\\boards\\hab\\advice\\advice19.htm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (HAB) supports the recommendation of the Tri-Party Public Involvement Strategy which calls for regularly scheduled public involvement planning meetings between agency staff and...

  2. Meetings

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    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  3. Reference Documents

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    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  4. Home

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    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  5. default

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  6. Notices

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    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  7. Slide 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Clean-Up Public Involvement Survey Results Survey Results Heart of America NW is conducting a survey to improve public involvement & information about Hanford Clean-up. 165...

  8. Stewardship on the Oak Ridge Reservation

    Office of Environmental Management (EM)

    the 15 recommendations were: Among the 15 recommendations were: 4. 4. DOE Public Involvement Plan should allow for DOE Public Involvement Plan should allow for public...

  9. bpa.gov - Transmission Services

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  10. FERC Filing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports & Tools Expand Reports & Tools Operations &...

  11. Business Practices

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sign In About | Careers | Contact | Investors | bpa.gov Search Doing Business Expand Doing Business Customer Involvement Expand Customer Involvement Reports &...

  12. Decades-long Mystery Solved | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Fenton reaction involves iron and hydrogen peroxide and is used to treat wastewater worldwide. Does the reaction involve a radical intermediate? Or, is it the...

  13. Audit Report: OAS-M-10-02

    Broader source: Energy.gov [DOE]

    Management Controls over Warranties Involving Newly Constructed and Renovated Facilities at National Defense Laboratories

  14. Email Template

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... 2 Public Involvement -- Tank Closure and Waste Management EIS (TC&WM EIS) ... 9 Committee Business......

  15. EIS-0236-S4: Record of Decision

    Broader source: Energy.gov [DOE]

    Complex Transformation—Operations Involving Plutonium, Uranium, and the Assembly and Disassembly of Nuclear Weapons

  16. Sixteenth International Conference on the physics of electronic and atomic collisions

    SciTech Connect (OSTI)

    Dalgarno, A.; Freund, R.S.; Lubell, M.S.; Lucatorto, T.B.

    1989-01-01

    This report contains abstracts of papers on the following topics: photons, electron-atom collisions; electron-molecule collisions; electron-ion collisions; collisions involving exotic species; ion- atom collisions, ion-molecule or atom-molecule collisions; atom-atom collisions; ion-ion collisions; collisions involving rydberg atoms; field assisted collisions; collisions involving clusters and collisions involving condensed matter.

  17. Investigation Report: I04RL003

    Broader source: Energy.gov [DOE]

    Investigation of Allegations Involving Occupational Medical Services and Tank Farm Vapor Exposures at the Hanford Site

  18. MEMORANDUM TO: FILE TYPE OF OPERATION

    Office of Legacy Management (LM)

    0 %EWtlE' INVOLVE?NT AT SITE ---...L------'------- ---- Control E %5idlth Physics Protection. 0 AECMED managed operations c; Little or None G AECMED responsible for c ...

  19. Risk Analysis

    Broader source: Energy.gov [DOE]

    Almost any new technology involves some risk. Risks involved in working with hydrogen can be minimized through adherence to standard design parameters for equipment and procedures. The Fuel Cell...

  20. Email Template

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    6, 2008 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATION COMMITTEE MEETING February 6, 2008 Pasco, WA Topics in this Meeting Summary Welcome and Introductions ............................................................................................................. 1 Public Involvement Workshop ........................................................................................................ 1 Tri-Party Agreement Quarterly Debrief

  1. Email Template

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1, 2009 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATION COMMITTEE MEETING April 1, 2009 Portland, OR Topics in this Meeting Summary Welcome and Introductions ............................................................................................................. 1 Approve meeting summary ............................................................................................................. 2 TPA Agencies Public Involvement Activities

  2. Email Template

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary, August 12, 2010 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATIONS COMMITTEE MEETING Aug 12, 2010 Richland, WA Topics in this Meeting Summary Welcome and introductions ................................................................................................ 1 EMAB - Open Meetings Advice ........................................................................................ 1 Strategic Public Involvement Planning Advice

  3. Draft Meeting Summary Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    February 2, 2016 DRAFT MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATION COMMITTEE February 2, 2016 Richland, WA Topics in this Meeting Summary Opening ......................................................................................................................................................... 1 Tri-Party Agreement Public Involvement ..................................................................................................... 2 HAB Report Summary

  4. Final Meeting Summary Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    April 7, 2015 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATION COMMITTEE April 7, 2015 Richland, WA Topics in this Meeting Summary Opening ......................................................................................................................................................... 1 TPA Public Involvement Survey .................................................................................................................. 2 WTP Communications

  5. Final Meeting Summary Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Committee September 4, 2013 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT & COMMUNICATION COMMITTEE September 4, 2013 Kennewick, WA Topics in this Meeting Summary Opening ......................................................................................................................................................... 1 Public Involvement Strategic Planning ......................................................................................................... 1 300

  6. Final Meeting Summary Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    September 8, 2015 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATION COMMITTEE September 8, 2015 Richland, WA Topics in this Meeting Summary Opening ......................................................................................................................................................... 1 TPA Public Involvement ............................................................................................................................... 1 TPA

  7. Final Meeting Summary Page 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    November 3, 2015 FINAL MEETING SUMMARY HANFORD ADVISORY BOARD PUBLIC INVOLVEMENT AND COMMUNICATION COMMITTEE November 3, 2015 Richland, WA Topics in this Meeting Summary Opening ......................................................................................................................................................... 1 Tri-Party Agreement Agency Public Involvement ....................................................................................... 2 Waste Treatment Plant

  8. file://L:\\DOE-hanford.gov\\public\\boards\\hab\\advice\\advice51.htm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Energy, Richland Operations PO Box 550 (A7-50) Richland, WA 99352 RE: A Public Involvement Process for the 10-Year Plan Dear Messrs. Alm and Wagoner: Public involvement is...

  9. RAPID/Roadmap/3-ID-a | Open Energy Information

    Open Energy Info (EERE)

    the IDL becomes involved only if they are notified and the Land Board requests their involvement. 3-ID-a.3 - Consultation (optional) IDL may meet with any other state agency to...

  10. Organizational Culture and Safety Culture: Are they one and the...

    Office of Environmental Management (EM)

    upward information flow won't exist in the safety aspect for very long. If employee involvement in planning is not a norm for the organizational culture - employee involvement in...

  11. DOE - Office of Legacy Management -- Teledyne-Columbia-Summerville...

    Office of Legacy Management (LM)

    1987 PA.01-1 Site Operations: Metal fabrication operations. No indication radioactive materials were involved. PA.01-1 Site Disposition: Eliminated - Site was not involved in the...

  12. CHAPTER 7

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... The power marketing administrations shall deposit ... In addition, interest DOE Financial Management Handbook ... or engineering services involving non-commercial ...

  13. Engaging Supply Chains in Energy Management

    Broader source: Energy.gov [DOE]

    Featuring presenters from PepsiCo and EUISSICA, this presentation covers ways of engaging industrial suppliers involved in energy management.

  14. BIO DZL | Open Energy Information

    Open Energy Info (EERE)

    DZL Place: Bangalore, Karnataka, India Product: Bangalore-based company involved in biodiesel production, jatropha development and biodiesel information dissemination....

  15. News Unsubscribe | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    contact public.affairs@nrel.gov. Mission & Programs Impact Leadership Community Education Center Community Involvement Economic Impact Sustainability Environment, Health, &...

  16. 2016 News | Community | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for 2016 yet. Archives 2015 | 2014 Mission & Programs Impact Leadership Community Education Center Community Involvement Economic Impact Sustainability Environment, Health, &...

  17. Richard Ahrenkiel - Research Fellow Emeritus | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    at the University of Illinois, Urbana. Mission & Programs Impact Leadership Community Education Center Community Involvement Economic Impact Sustainability Environment, Health, &...

  18. Solar Easements

    Broader source: Energy.gov [DOE]

    These are minimum requirements, so additional points of agreement may be added at the discretion of those involved.

  19. Idaho

    Broader source: Energy.gov [DOE]

    Following are links to compliance agreements involving the Idaho site. Brief summaries of the agreements also are included.

  20. GeoChips for Analysis of Microbial Functional Communities (Book...

    Office of Scientific and Technical Information (OSTI)

    that contain probes for genes encoding proteins or enzymes involved in functions of ... GENES; HYBRIDIZATION; PROBES; PROTEINS; WASHING Microbial Functional ...

  1. Cleaning Up the Hanford River Corridor and Improving Site Operations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    * History and Regulatory Background * Permitting Background * Proposed Permit ... Administrative Code (WAC) regulatory requirements for public involvement (WAC ...

  2. Radiological Assessor Training

    Energy Savers [EERE]

    ... highest levels, line management involvement and ... level as low as reasonably achievable (ALARA). * Incorporate dose reduction, contamination reduction, and waste ...

  3. Docket No. EERE–2010–BT–STD–0027 Energy Conservation Standards for Commercial and Industrial Electric Motors: Public Meeting and Availability of the Preliminary Technical Support Document 77 Fed. Reg. 43015 (July 23, 2012)

    Broader source: Energy.gov [DOE]

    This memorandum memorializes a communication involving members of the Motor Coalition (industry and energy advocates) in connection with this proceeding.

  4. Solar Powering Your Community: A Guide for Local Governments...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... that Appropriately Value Solar . . . . . . . . . . . ... . . . . . . . . . 137 Tax Credit Bond Financing . . . . . . ... because they can reduce the risk and complexity involved in ...

  5. Search results | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    eereeducationdownloadsexploration-wind-energy-wind-turbines Download Creating Biodiesel & Mitigating Waste Safety practices for handling the materials involved in producing...

  6. BTS: SEP How To Guide: Quantify Impacts of Environmental Businesses

    Broader source: Energy.gov [DOE]

    This activity involves gathering data on the energy and environmental businesses in the State, including the revenue generated.

  7. Democratic Peoples Republic of Korea Initiative, Export Control Issues for the Proposed PNNL Energy Efficiency Center in North Korea

    SciTech Connect (OSTI)

    Rither, Alan C.; Varley, Darlene A.

    2001-01-10

    This is a letter report provided to program participants involved in developing the Energy Efficiency Center in the DPRK.

  8. Sandia National Laboratories: News: Publications: Lab Accomplishments

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bioscience Community involvement, customers relations, and institutional development ... and management Homeland security HR & Finance IT, networks, and facilities Materials ...

  9. NREL: Biomass Research - Biochemical Conversion Projects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL's projects in biochemical conversion involve three ... yeast and bacteria) Processing the fermentation product ... Bioprocess Integration Researchers are refining a ...

  10. EIS-0281-SA-02: Supplement Analysis

    Broader source: Energy.gov [DOE]

    Sandia National Laboratories/New Mexico Isentropic Compression and Flyer Plate Experiments Involving Plutonium at the Z and Saturn Accelerators

  11. Microsoft PowerPoint - Volvo WEEC 2012.pptx

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the area of Communications, increases the environmental awareness, involvement and empowerment - Strengthens the environmental design and procurement process - Strengthens the ...

  12. Patterns in wetland microbial community composition and functional...

    Office of Scientific and Technical Information (OSTI)

    and methane production, including diverse microorganisms involved in methane production and consumption. We found that methanogenesis gene abundance is inversely...

  13. Making Buildings Better: Indie Energy & the Geothermal Breakthrough

    Broader source: Energy.gov [DOE]

    Read about Indie Energy -- one of several clean energy small businesses involved in the "Entrepreneurial Mentor Corps."

  14. Docket No’s EERE–2010–BT–TP–0044 and EERE–2010–BT–STD–0043 Energy Conservation Program: Energy Conservation Standards and Test Procedures for HID Lamps

    Broader source: Energy.gov [DOE]

    This memorandum memorializes a communication involving NEMA Lamp Section members and DOE staff in connection with this proceeding.

  15. Environmental Laws and Regulations | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Record Files Natural Resource Damage Assessments Environmental Laws and Regulations More Documents & Publications Paducah Public Involvement Plan Portsmouth Removal Actions...

  16. 6Li General Tables

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Li General Table The General Table for 6Li is subdivided into the following categories: Ground State Properties of 6Li Special States Theoretical Shell Model Cluster Models Complex Reactions Involving 6Li Model Calculations Electromagnetic Transitions Muon and Neutrino Capture and Reactions Reactions Involving Pions, Other Mesons and Baryon States Light-ion and Neutron Induced Reactions Pions Hypernuclei Reactions Involving Antiprotons Astrophysics

  17. Report to Congress on abnormal occurrences, October--December 1993. Volume 16, No. 4

    SciTech Connect (OSTI)

    Not Available

    1994-04-01

    Section 208 of the Energy Reorganization Act of 1974 identifies an abnormal occurrence as an unscheduled incident or event that the Nuclear Regulatory Commission determines to be significant from the standpoint of public health or safety and requires a quarterly report of such events to be made to Congress. This report covers the period from October 1 through December 31, 1993. This report discusses six abnormal occurrences at NRC-licensed facilities. Five involved medical brachytherapy misadministrations, and one involved an overexposure to a nursing infant. Seven abnormal occurrences that were reported by the Agreement States are also discussed, based on information provided by the Agreement States as of February 28, 1994. Of these events, three involved brachytherapy misadministrations, one involved a teletherapy misadministration, one involved a theft of radioactive material during transport and improper disposal, and two involved lost sources.

  18. Upper Los Alamos Canyon Cleanup

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Upper Los Alamos Canyon Cleanup Upper Los Alamos Canyon Cleanup The Upper Los Alamos Canyon Project involves cleaning up hazardous materials left over from some of the Laboratory's earliest activities. Contact Environmental Communication & Public Involvement P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505) 667-0216 Email Located along Los Alamos Canyon from 7th Street to the Pajarito Ski Hill, the Upper Los Alamos Canyon Project involves examining sites in present and former Laboratory

  19. Exploring the Genome and Proteome of Desulfitobacterium hafniense DCB2 for

    Office of Scientific and Technical Information (OSTI)

    its Protein Complexes Involved in Metal Reduction and Dechlorination (Technical Report) | SciTech Connect Exploring the Genome and Proteome of Desulfitobacterium hafniense DCB2 for its Protein Complexes Involved in Metal Reduction and Dechlorination Citation Details In-Document Search Title: Exploring the Genome and Proteome of Desulfitobacterium hafniense DCB2 for its Protein Complexes Involved in Metal Reduction and Dechlorination Desulfitobacteria are of interest to DOE mission because of

  20. Multiplet resonance lifetimes in resonant inelastic x-ray scattering

    Office of Scientific and Technical Information (OSTI)

    involving shallow core levels (Journal Article) | SciTech Connect Multiplet resonance lifetimes in resonant inelastic x-ray scattering involving shallow core levels Citation Details In-Document Search Title: Multiplet resonance lifetimes in resonant inelastic x-ray scattering involving shallow core levels Authors: Wray, L. Andrew ; Yang, Wanli ; Eisaki, Hiroshi ; Hussain, Zahid ; Chuang, Yi-De Publication Date: 2012-11-19 OSTI Identifier: 1101794 Type: Publisher's Accepted Manuscript Journal

  1. U.S. Department of Energy Portsmouth/Paducah Project Office

    Energy Savers [EERE]

    The activities involve utility and electrical system optimization at the U.S. Department of Energy (DOE) Portsmouth Gaseous Diffusion Plant (PORTS), Piketon, Ohio. It encompasses utility optimization involving both water related and electrical and communication systems needed to provide more efficient systems and more flexibility for plant operations. Also encompasses activities involving isolating systems that are no longer needed or required and the changes will make use of existing

  2. Enforcement Guidance Supplement 98-02: DOE Enforcement Activities where Off-site Transportation Issues are also Present.

    Broader source: Energy.gov [DOE]

    Recently several questions have arisen regarding the scope of Price-Anderson enforcement when transportation issues are directly or indirectly involved in an incident. These questions can be separated into two areas, (1) transportation issues that involve on-site transportation typically not regulated by the Department of Transportation (DOT), and (2) transportation issues that involve off-site transportation. This guidance addresses off-site transportation that is regulated by DOT and other state and federal agencies.

  3. Characterization and structure of DhpI, a phosphonate O-methyltransferase

    Office of Scientific and Technical Information (OSTI)

    involved in dehydrophos biosynthesis (Journal Article) | SciTech Connect Journal Article: Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis Citation Details In-Document Search Title: Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis Phosphonate natural products possess a range of biological activities as a consequence of their ability to mimic phosphate esters or

  4. Purification, crystallization and structural elucidation of

    Office of Scientific and Technical Information (OSTI)

    D-galactaro-1,4-lactone cycloisomerase from Agrobacterium tumefaciens involved in pectin degradation (Journal Article) | SciTech Connect Purification, crystallization and structural elucidation of D-galactaro-1,4-lactone cycloisomerase from Agrobacterium tumefaciens involved in pectin degradation Citation Details In-Document Search Title: Purification, crystallization and structural elucidation of D-galactaro-1,4-lactone cycloisomerase from Agrobacterium tumefaciens involved in pectin

  5. F:\SHARE\SE\Web_Origs\Wrk_Jan\mvsowp\MONPIP.PDF

    Office of Legacy Management (LM)

    7.1 UMTRA Ground Water Project Public Involvement Plan for the Environmental Assessment of Ground Water Compliance at the Monument Valley, Arizona, Uranium Mill Tailings Site July 1999 Prepared by U.S. Department of Energy Grand Junction Office Grand Junction, Colorado Work performed under DOE Contract No. DE-AC13-96GJ87335 Public Involvement Plan for the Monument Valley UMTRA Site Page 2 Introduction This Public Involvement Plan is tiered to the Uranium Mill Tailings Remedial Action (UMTRA)

  6. EIS-0355: Notice of Intent to Prepare an Environmental Impact Statement and

    Office of Environmental Management (EM)

    to Conduct Public Scoping Meetings and Notice of Floodplain and Wetlands Involvement | Department of Energy Notice of Intent to Prepare an Environmental Impact Statement and to Conduct Public Scoping Meetings and Notice of Floodplain and Wetlands Involvement EIS-0355: Notice of Intent to Prepare an Environmental Impact Statement and to Conduct Public Scoping Meetings and Notice of Floodplain and Wetlands Involvement Remediation of the Moab Uranium Mill Tailings Site in Grand County, UT DOE

  7. EA-1915: Notice of Intent to Prepare an Environmental Assessment and Notice

    Energy Savers [EERE]

    of Potential Floodplain and Wetland Involvement | Department of Energy Notice of Intent to Prepare an Environmental Assessment and Notice of Potential Floodplain and Wetland Involvement EA-1915: Notice of Intent to Prepare an Environmental Assessment and Notice of Potential Floodplain and Wetland Involvement Proposed Conveyance of Land at the Hanford Site, Richland, Washington DOE is announcing its intent to prepare an EA to assess the potential environmental effects of conveying

  8. EIS-0372: Notice of Intent to Prepare an Environmental Impact Statement and

    Energy Savers [EERE]

    to Conduct Public Scoping Meetings and Notice of Floodplain and Wetlands Involvement | Department of Energy Notice of Intent to Prepare an Environmental Impact Statement and to Conduct Public Scoping Meetings and Notice of Floodplain and Wetlands Involvement EIS-0372: Notice of Intent to Prepare an Environmental Impact Statement and to Conduct Public Scoping Meetings and Notice of Floodplain and Wetlands Involvement Bangor Hydro-Electric Company Bangor Hydro-Electric Company (BHE) has

  9. 10-23-2002summary.doc

    Office of Legacy Management (LM)

    Summary of Weldon Spring Focus Area Work Session October 23, 2002 Weldon Spring Interpretive Center Focus Area: Communication and Public Involvement Purpose and Goals David Geiser, Director of the Office of Long-Term Stewardship, referred to the previous public meetings held in June and August as being Phase I of the Long-Term Stewardship Plan process. Phase II involves three focus area work sessions: 1) communication and public involvement, 2) institutional controls and homeland security, and

  10. Report of the Committee on Antitrust. I. Coal

    SciTech Connect (OSTI)

    Not Available

    1985-01-01

    The Committee reports on the background and outcomes of antitrust cases in the energy field. The report covers four antitrust cases involving coal, six decisions and six recently filed cases involving electric utilities, three cases involving oil, and five decisions as well as nine recently filed natural gas antitrust cases. Issues which led to the suits include transport, price contracts, and conspiracies in restraint of trade.

  11. gjpip.PDF

    Office of Legacy Management (LM)

    GWGRJ 7.1 Public Involvement Plan for the Environmental Assessment of Ground Water Compliance at the Grand Junction Uranium Mill Tailings Remedial Action (UMTRA) Project Site (Climax Uranium Millsite) July 1999 Prepared by U.S. Department of Energy Grand Junction Office Grand Junction, Colorado Work performed under DOE Contract No. DE-AC13-96GJ8733 Public Involvement Plan July 1999 Page 1 Public Involvement Plan for the Environmental Assessment of Ground Water Compliance at the Grand Junction,

  12. PROJECT MANGEMENT PLAN EXAMPLES Prepare Project Support Plans and

    Office of Environmental Management (EM)

    Communication and Stakeholder Involvement Plan Examples Example 49 10.0 COMMUNICATIONS AND PUBLIC INVOLVEMENT The transition of B Plant is a critical element in Hanford's mission of environmental management. The B Plant Transition Project Management Team have made a commitment to open communications throughout transition because effective communications and public involvement are critical success factors for the project. Communications must be living and dynamic, responding to accomplishments

  13. Distribution: Sonya Baskerville, Bonneville Power Administration...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Predictive Maintenance. ORD Those activities involving continuous or periodic monitoring and diagnosis to forecast component degradation so that "as needed" maintenance can be ...

  14. Grainger: Compromise Agreement (2013-SE-1411)

    Broader source: Energy.gov [DOE]

    DOE and Grainger International Inc. entered into a Compromise Agreement to resolve a case involving the distribution in commerce of noncompliant freezers.

  15. ENERGY EFFICIENCY AND CONSERVATION BLOCK GRANTS PROGRAM NOTICE 10-012

    Broader source: Energy.gov [DOE]

    DAVIS-BACON ACT WAGE RATES FOR ARRA-FUNDED ENERGY EFFICIENCY AND CONSERVATION BLOCK GRANTS PROGRAM PROJECTS INVOLVING RESIDENTIAL WEATHERIZATION WORK

  16. SANDIA CORPORATION SF 6432CI (0305) SECTION II

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... THAT MAY INVOLVE ACCESS TO CLASSIFIED INFORMATION DEAR 952.2042 Security Requirements DEAR 952.20470 ClassificationDeclassification DEAR 970.52041 Counterintelligence CI27 APPLY ...

  17. Docket No's EERE-2010-BT-TP-0044 andEERE-2010-BT-STD-0043 Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Standards and Test Procedures for HID Lamps This memorandum memorializes a communication involving NEMA Lamp Section members and DOE staff in connection with this proceeding. ...

  18. Ex Parte Communications in Connection with Docket No'sEERE-2015...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This memorandum memorializes a communication involving NEMA Staff and DOE staff in connection with this proceeding on March 3, 2016. The National Electrical Manufacturers ...

  19. Pax Global: Compromise Agreement (2013-SE-1413)

    Broader source: Energy.gov [DOE]

    DOE and Pax Global, Inc., entered into a Compromise Agreement to resolve a case involving the distribution in commerce of noncompliant freezers.

  20. BPA-2015-00190-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    involved still exist and where. The FOIA clearly states that records must exist at the time the request is submitted. Enter description: Miller Act Bond (PerformancePayment...

  1. Infrastructure Security EXCEPTIONAL SERVICE IN THE NATIONAL INTEREST

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for the nuclear power industry are ... Generation Nuclear Plants (NGNP), including high-tempurature gas cooled reactors (HTGR). ... involving risk, safety, and security. ...

  2. Characterization of the Installed Costs of Prime Movers Using...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Supplementary costs are often involved with the installation of power generating equipment utilizing opportunity fuels. In particular, landfill gas (LFG) and anaerobic digester gas ...

  3. Share Your Clean Energy Economy Story

    Broader source: Energy.gov [DOE]

    How did you get involved in the Clean Energy Economy? Help other people learn the opportunities available in the clean energy sector by sharing your own story below.

  4. Enzyme Structure Provides Insights into Cancer and Aging

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    protein are responsible for three different human diseases: in xeroderma pigmentosum (XP), extreme sensitivity to sunlight promotes cancer; Cockayne syndrome (CS) involves...

  5. Iberdrola Renewables formerly PPM Energy Inc | Open Energy Information

    Open Energy Info (EERE)

    Wind energy Product: Oregon-based Iberdrola Renewables is the US development arm of Spanish project developer Iberdrola Renovables. It is actively involved in wind power...

  6. Swiss Park Vanijya Pvt Ltd | Open Energy Information

    Open Energy Info (EERE)

    Park Vanijya Pvt Ltd Jump to: navigation, search Name: Swiss Park Vanijya Pvt Ltd Place: Mumbai, Maharashtra, India Sector: Solar Product: Mumbai-based firm involved in solar...

  7. DOE Announces Selections for SSL R&D Funding Opportunity | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    projects will focus on the areas of Core Technology Research, which involves the ... This is the ninth round of DOE funding for SSL Core Technology Research and Product ...

  8. NEPA of 1969

    Broader source: Energy.gov (indexed) [DOE]

    ... and develop methods and procedures, in consultation with the ... with respect to any environmental impact involved. Copies of such statement and the comments and views of ...

  9. DOE Explosives Safety Manual

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1996-03-29

    This Manual describes DOE's explosives safety requirements applicable to operations involving the development, testing, handling, and processing of explosives or assemblies containing explosives.

  10. Chinese Geological Survey | Open Energy Information

    Open Energy Info (EERE)

    Chinese Geological Survey Jump to: navigation, search Name: Chinese Geological Survey Place: China Sector: Geothermal energy Product: Chinese body which is involved in surveys of...

  11. Richmond Field Station Tow Tank | Open Energy Information

    Open Energy Info (EERE)

    Real-Time Yes Data Comparison to Computational Models Numerous PhD theses involved experimental components that were carried out at the facility Integrated DisplayGraphics...

  12. 3-3-2010_Glenn_Podonsky_Final_Testimony(1).pdf

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    force personnel. Study Group recommendations dealing with protective force physical fitness standards, medical requirements, and the Human Reliability Program all involve...

  13. DOE F 3305.14f | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Form provides information related to managerial competencies that focuses on involvement in the process and systems used to monitor program development, policy implementation, and ...

  14. C:\\Forms\\DOE F 3305.14f.cdr

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CONTENT DESCRIPTION: Focuses on involvement in the process and systems used to monitor program develop- ment, policy implementation, and program effectiveness. Also includes other ...

  15. Presidential Proclamation on the 40th Anniversary of the National...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of President Obama to "call upon all executive branch agencies to promote public involvement and transparency in their implementation of the National Environmental Policy Act." ...

  16. Institute for Atom-Efficient Chemical Transformations Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    will involve synthesis, characterization, and catalytic reactor testing of new materials. The Postdoctoral Appointee will be working with a larger group from Argonne and...

  17. Physical Organic Chemistry of Reactive Intermediates | The Ames...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by the photolysis of sulfur bearing precursors. Techniques and approaches involve synthesis, spectroscopy, and computational chemistry. The group also has some interest in...

  18. EA-1925: Final Environmental Assessment | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    geothermal projects which involve drilling up to 16 wells for geothermal ... south of U.S. Highway 20. The proposed project includes three distinct drilling areas. ...

  19. NREL: Energy Analysis - Stuart Cohen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ReEDS) Optimizing complex systems involving science, technology, economics, and policy ... Off in Response to Electric Grid Demand: A Baseline Analysis of Emissions and Economics." ...

  20. Lizana Pierce | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    in the defense and aerospace industries and was fortunate to have been involved in the integrated science payload flown on the Tethered Satellite Shuttle Mission (STS-46). Ms. ...

  1. Inspection Report: INS-O-14-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    These allegations involve explosives-related work performed at LLNL for a National Geographic documentary about the Lusitania, a British passenger ship that sank in May 1915. Our ...

  2. Sinia Renovables SCR | Open Energy Information

    Open Energy Info (EERE)

    Name: Sinia Renovables SCR Place: Spain Sector: Renewable Energy Product: Investment arm of Banco Sabadell, involved in the financing of renewable energy projects. References:...

  3. Microsoft Word - PSH-12-0098.docx

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Individual testified that upon further reflection, he believes that he only took the ... the lessons learned presentation involving introspection and reflection of his errors. ...

  4. Frontiers in Advanced Storage Technologies (FAST) project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the product for use in HPC environments. The FAST project involves establishing long-term development collaboration agreements to develop the following opportunities: File...

  5. Microsoft Word - Sand_2009_0821_P_Construction_Rigor.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of new equipment not previously used by the laboratoryfacility that will be connected to standard utilities * Work involving additional hazards (acute chemical hazards such as...

  6. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... involved in development of novel scintillating crystals and has proposed several crystal based detector concepts for future HEP experiments at the energy and intensity frontiers. ...

  7. The Safe Handling of Unbound Engineered Nanoparticles

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-05-31

    The order establishes requirements and assigns responsibilities for activities involving unbound engineered nanoparticles (UNP). Admin Chg 1, dated 2-14-13, supersedes DOE O 456.1.

  8. SEMINAR ANNOUNCEMENTS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    His research involved a variety of topics in spintronics, semiconductor physics, strongly correlated materials, and development of firstUprinciples methods for the electronic ...

  9. Jimmy Bell

    Broader source: Energy.gov [DOE]

    Jimmy currently provides consulting services to Egan, Fitzpatrick, Malsch & Lawrence, PLLC, a legal firm involved in environmental issues. Previously, he worked as a chemist at Oak Ridge...

  10. Minrav Holdings | Open Energy Information

    Open Energy Info (EERE)

    Minrav Holdings Jump to: navigation, search Name: Minrav Holdings Place: Israel Product: Minrav Holdings Ltd. is involved in initiating, engineering and building real estate,...

  11. A=11Be (2012KE01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0.8. The data related to states involved in neutron emission have ambiguous interpretation connected with uncertainty in placement of neutron decay branches; the observed...

  12. Cha-Ching! How One Missouri Town is Helping Residents Save Money...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    cities in America -- prides itself on having a "Green City" philosophy. This includes getting the community active and involved through energy conservation outreach and education. ...

  13. FE - Fossil Energy - Energy Conservation Plan

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The focus of this plan is on how FE employees can change their behavior to reduce energy ... employee involvement. * Start an educational campaign that allows employees to ...

  14. New season of colloquia begins at Princeton Plasma Physics Laboratory...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    This year Stratton hopes to bring in speakers who aren't necessarily involved in plasma physics, perhaps scientists who research dark matter or cosmology. Mikkelsen is a...

  15. Magnetorotational Instability (MRI) experiment | Princeton Plasma...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Currently, scientists do not understand the required conditions and the accretion, or matter collection process, involved in star and planet formation. But the Magnetorotational...

  16. Army Awards 20 Additional Contracts for Renewable Energy Technologies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    awarded another 20 base contracts to companies for renewable energy-related technologies. ... to be developed by the approved companies, and involve no Army or Department of ...

  17. MEMORANDUM

    Office of Legacy Management (LM)

    AECMED INVOLVEMENT AT SITE -------------... Control 0 Health Physics Protection D CIEWMED managed operations 0 Little or None D AEWMED responsible for c ...

  18. MEMORANDUM DATE

    Office of Legacy Management (LM)

    E FINCIL PRODUCT W4STE & RESIDUE . AEC,' MED INVOLVEMENT CIT 5 I TE ---...---... Control w Health Physics Protection 0 CIECMED managed operations (-J Little or None ...

  19. TO: FILE GiR FROM: SUBJECT: I OWNER(S) Past: Current:

    Office of Legacy Management (LM)

    - '-'-k. FECMED INVOLVE%ENr AT SITE --.---....---....+ Control ---% Health Physics Protec G AECMED managed operations q t Little or None ci AECMED respansible far ...

  20. MEIIORANDUM TO: FILE

    Office of Legacy Management (LM)

    INVOLVE?EXT AT SITE ---- . - . ;------ ------ - i *. .u? . .x . Control cj Htiilth Physics Protection . 0 AECMED managed operations 0 Little or None G AECMED responsible ...

  1. MEMORANDU TO: FILE FHOM: SUBJECT:

    Office of Legacy Management (LM)

    1 .., ' . AECMED INVOLVEMENT AT SITE ----------------- Control q Health Physics Protection ' 0 AEWMED managed operations r Little or None 0 AECMED responsible for c ...

  2. MEMORANDUM

    Office of Legacy Management (LM)

    ...MED INVOLVEMENT AT SITE ,-- - -e--m- ------v--e---- Control I 0 0 iit 9( Health Physics Protection AEWMED managed operations AEWMED responsible for accountability AEWMED ...

  3. Q MEtlORANDUM SUBJECT:

    Office of Legacy Management (LM)

    AECMED INVOLVEMENT AT SITE -...---... ------ . , Control 0 Health Physics Protection 0 AEWMED managed operations (J Little or None 0 AEWMED responsible for -ECNED ...

  4. MEHORANDUM TO: FILE

    Office of Legacy Management (LM)

    ' I* ,: * - ' &eMED INVOLVEMENT AT SITE ---------------- Control 0 Health Physics Protection Q AECMED managed operations D Little or None 0 AECMED responsible for f ...

  5. OWNER(S)

    Office of Legacy Management (LM)

    BUILDINGS EQUIPMENT ORE OR RAW HATL 0 FINAL PRODUCT B WASTE & RESIDUE @ . .,' ' -. . ?t ' AEWME; INVOLVEMENT AT SITE ---------------- Control Health Physics Protection . ...

  6. MEMORANDUM TO: FILE F3on: A 6 IL

    Office of Legacy Management (LM)

    .Z,+,ECI-INVOLVE?EXT AT SITE . ---... ---... ---- . 1, Control c Health Physics Protection . 0 AECMED managed operations G Little or None 3 AECtlED responsible for ...

  7. Enforcement Letter, WEL-2014-02- April 17, 2014

    Broader source: Energy.gov [DOE]

    Issued to B&W Pantex, LLC, related to six events involving improper management, handling, or labeling of highly hazardous materials, including explosives, at DOE's Pantex Plant.

  8. McSlarrow Letter - March 9, 2004

    Office of Environmental Management (EM)

    following actions regarding public participation and involvement: * Following the DOE's Public Participation and Community Policy (DOE P 141.2) on public outreach and...

  9. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... We have applied the same modern principles of involved site radiation therapy as ... Sonny ; Petriello, Frank ; High Energy Physics Division, Argonne National Laboratory, ...

  10. WBA-14-0001- In the Matter of Krishnan Balasubramanian

    Broader source: Energy.gov [DOE]

    On December 28, 2015, OHA denied an Appeal involving a Complaint filed by Krishnan Balasubramanian (Balasubramanian) against Lawrence Livermore National Security (LLNS), management and operating...

  11. NREL Launches Strategic Energy Analysis Institute - News Releases...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The U.S. Department of Energy's National Renewable Energy Laboratory (NREL) announced today ... It involves the market structure, law, natural resource use, the impact on local and ...

  12. Peter_Lyons_Final_Testimony.pdf

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Subcommittee on Environment and the Economy U.S. House of Representatives "The ... Sweden, and France, where public involvement and consultation are heavily emphasized. ...

  13. Uttarakhand Jal Vidyut Nigam Ltd UJVNL | Open Energy Information

    Open Energy Info (EERE)

    Sector: Hydro Product: Dehradun-based corporation involved in construction, operation and managment of hydro power development & generation. Coordinates: 30.3315, 78.03057 Show...

  14. Ryan Egidi | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    These projects primarily involve advanced metering infrastructure, communication infrastructure, distribution automation, demand management and cybersecurity. Ryan has a B.S. in ...

  15. B53 Nuclear Bomb | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    weapon system with the facility, tooling, operating procedures, and personnel involved in the dismantlement program to form a safe, efficient, and effective operating environment. ...

  16. Baltimore County, Maryland ASHRAE 169-2006 Climate Zone | Open...

    Open Energy Info (EERE)

    Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit History Baltimore County, Maryland ASHRAE 169-2006 Climate Zone Jump to: navigation, search County...

  17. Baltimore City County, Maryland ASHRAE 169-2006 Climate Zone...

    Open Energy Info (EERE)

    Get Involved Help Apps Datasets Community Login | Sign Up Search Page Edit History Baltimore City County, Maryland ASHRAE 169-2006 Climate Zone Jump to: navigation, search...

  18. URS Corporation | Open Energy Information

    Open Energy Info (EERE)

    Name: URS Corporation Place: San Francisco, California Zip: 94111-2728 Sector: Solar Product: Engineering and design contractor involved in building biodiesel plants and solar...

  19. Slide 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    analysis * Separate analyses required for potential land conveyance * How to give inputstay involved 3 Public Scoping Meeting Purposes * Provide information about what DOE will...

  20. Regional Study to Guide Policy Interventions for Enhancing the...

    Open Energy Info (EERE)

    priorities, to be addressed through Research and Technology Development (R&TD). Business is primarily involved as user of ESTs, and can be actively encouraged to adopt...