National Library of Energy BETA

Sample records for induced adaptive response

  1. The innate and adaptive immune response induced by alveolar macrophages exposed to ambient particulate matter

    SciTech Connect (OSTI)

    Miyata, Ryohei; Eeden, Stephan F. van

    2011-12-15

    Emerging epidemiological evidence suggests that exposure to particulate matter (PM) air pollution increases the risk of cardiovascular events but the exact mechanism by which PM has adverse effects is still unclear. Alveolar macrophages (AM) play a major role in clearing and processing inhaled PM. This comprehensive review of research findings on immunological interactions between AM and PM provides potential pathophysiological pathways that interconnect PM exposure with adverse cardiovascular effects. Coarse particles (10 {mu}m or less, PM{sub 10}) induce innate immune responses via endotoxin-toll-like receptor (TLR) 4 pathway while fine (2.5 {mu}m or less, PM{sub 2.5}) and ultrafine particles (0.1 {mu}m or less, UFP) induce via reactive oxygen species generation by transition metals and/or polyaromatic hydrocarbons. The innate immune responses are characterized by activation of transcription factors [nuclear factor (NF)-{kappa}B and activator protein-1] and the downstream proinflammatory cytokine [interleukin (IL)-1{beta}, IL-6, and tumor necrosis factor-{alpha}] production. In addition to the conventional opsonin-dependent phagocytosis by AM, PM can also be endocytosed by an opsonin-independent pathway via scavenger receptors. Activation of scavenger receptors negatively regulates the TLR4-NF-{kappa}B pathway. Internalized particles are subsequently subjected to adaptive immunity involving major histocompatibility complex class II (MHC II) expression, recruitment of costimulatory molecules, and the modulation of the T helper (Th) responses. AM show atypical antigen presenting cell maturation in which phagocytic activity decreases while both MHC II and costimulatory molecules remain unaltered. PM drives AM towards a Th1 profile but secondary responses in a Th1- or Th-2 up-regulated milieu drive the response in favor of a Th2 profile.

  2. Beyond Adapting to Climate Change: Embedding Adaptation in Responses to Multiple Threats and Stresses

    SciTech Connect (OSTI)

    Wilbanks, Thomas J; Kates, Dr. Robert W.

    2010-01-01

    Climate change impacts are already being experienced in every region of the United States and every part of the world most severely in Arctic regions and adaptation is needed now. Although climate change adaptation research is still in its infancy, significant adaptation planning in the United States has already begun in a number of localities. This article seeks to broaden the adaptation effort by integrating it with broader frameworks of hazards research, sustainability science, and community and regional resilience. To extend the range of experience, we draw from ongoing case studies in the Southeastern United States and the environmental history of New Orleans to consider the multiple threats and stresses that all communities and regions experience. Embedding climate adaptation in responses to multiple threats and stresses helps us to understand climate change impacts, themselves often products of multiple stresses, to achieve community acceptance of needed adaptations as co-benefits of addressing multiple threats, and to mainstream the process of climate adaptation through the larger envelope of social relationships, communication channels, and broad-based awareness of needs for risk management that accompany community resilience.

  3. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    SciTech Connect (OSTI)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  4. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  5. the Adaptive Response, Genetic Haplo-Insufficiency and Genomic Instability

    SciTech Connect (OSTI)

    Geard, Charles R.

    2014-12-12

    The linear no-threshold (LNT) hypothesis is the driving force in the establishment of radiation protection standards. However, the scientific basis for linearity has been brought into question, particularly due to the concerns about induced radiation resistance as it pertains to oxidative stress. Specifically, we investigated the observation that tumor hypoxia is associated with malignant progression, increased metastases, chemo- and radioresistance and poor prognosis. Experiments were conducted with non-malignant 3T3/NIH cells and normal human lung fibroblasts (NHLF) that were subjected to ?-irradiation under the levels of oxygen resembling those in growing tumors, and related our data to the concentrations of dissolved oxygen (DO), which is a better indicator of the amounts of residual oxygen inside the cells cultured in the hypoxic or anoxic atmosphere. We found that at DO levels about 0.5 mg/L cells subjected to both short-term (17 hours) and prolonged (48-72 hours) hypoxia continued to proliferate, and that apoptotic events were decreased at the early hours of hypoxic treatment. We showed that the short-term hypoxia up-regulated p53-binding protein 1 (53BP1) and resulted in facilitated 53BP1 nuclear foci formation and disappearance, thus indicating the higher efficiency of DNA double strand breaks repair processes. The latter was confirmed by the lower micronuclei incidence in irradiated hypoxic cells.

  6. Systematic identification of genes and transduction pathways involved in radio-adaptive response

    SciTech Connect (OSTI)

    Wu, Honglu

    2015-05-22

    Low doses of radiation have been shown to protect against the biological effects of later exposure to toxic levels of radiation. In this study, we propose to identify the molecular mechanisms of this adaptive response by systematically identifying the genes that play a role in radio-protection. In the original proposal, a human cell line that is well-documented to exhibit the radio-adaptive effect was to be used. In this revised study plan, we will use a mouse model, C57BL/6, which has also been well investigated for radio-adaptation. The goal of the proposed study is to enhance our understanding of cellular responses to low doses of radiation exposure at the molecular level.

  7. Scientists in a Changed Institutional Environment: Subjective Adaptation and Social Responsibility Norms in Russia

    SciTech Connect (OSTI)

    Gerber, T P; Ball, D Y

    2008-06-05

    How do scientists react when the institutional setting in which they conduct their work changes radically? How do long-standing norms regarding the social responsibility of scientists fare? What factors influence whether scientists embrace or reject the new institutions and norms? We examine these questions using data from a unique survey of 602 scientists in Russia, whose science system experienced a sustained crisis and sweeping changes in science institutions following the collapse of the Soviet Union. We develop measures of how respondents view financing based on grants and other institutional changes in the Russian science system, as well as measures of two norms regarding scientists social responsibility. We find that the majority of scientists have adapted, in the sense that they hold positive views of the new institutions, but a diversity of orientations remains. Social responsibility norms are common among Russian scientists, but far from universal. The main correlates of adaptation are age and current success at negotiating the new institutions, though prospective success, work context, and ethnicity have some of the hypothesized associations. As for social responsibility norms, the main source of variation is age: younger scientists are more likely to embrace individualistic rather than socially-oriented norms.

  8. Arsenic transformation predisposes human skin keratinocytes to UV-induced DNA damage yet enhances their survival apparently by diminishing oxidant response

    SciTech Connect (OSTI)

    Sun Yang; Kojima, Chikara; Chignell, Colin; Mason, Ronald; Waalkes, Michael P.

    2011-09-15

    Inorganic arsenic and UV, both human skin carcinogens, may act together as skin co-carcinogens. We find human skin keratinocytes (HaCaT cells) are malignantly transformed by low-level arsenite (100 nM, 30 weeks; termed As-TM cells) and with transformation concurrently undergo full adaptation to arsenic toxicity involving reduced apoptosis and oxidative stress response to high arsenite concentrations. Oxidative DNA damage (ODD) is a possible mechanism in arsenic carcinogenesis and a hallmark of UV-induced skin cancer. In the current work, inorganic arsenite exposure (100 nM) did not induce ODD during the 30 weeks required for malignant transformation. Although acute UV-treatment (UVA, 25 J/cm{sup 2}) increased ODD in passage-matched control cells, once transformed by arsenic to As-TM cells, acute UV actually further increased ODD (> 50%). Despite enhanced ODD, As-TM cells were resistant to UV-induced apoptosis. The response of apoptotic factors and oxidative stress genes was strongly mitigated in As-TM cells after UV exposure including increased Bcl2/Bax ratio and reduced Caspase-3, Nrf2, and Keap1 expression. Several Nrf2-related genes (HO-1, GCLs, SOD) showed diminished responses in As-TM cells after UV exposure consistent with reduced oxidant stress response. UV-exposed As-TM cells showed increased expression of cyclin D1 (proliferation gene) and decreased p16 (tumor suppressor). UV exposure enhanced the malignant phenotype of As-TM cells. Thus, the co-carcinogenicity between UV and arsenic in skin cancer might involve adaptation to chronic arsenic exposure generally mitigating the oxidative stress response, allowing apoptotic by-pass after UV and enhanced cell survival even in the face of increased UV-induced oxidative stress and increased ODD. - Highlights: > Arsenic transformation adapted to UV-induced apoptosis. > Arsenic transformation diminished oxidant response. > Arsenic transformation enhanced UV-induced DNA damage.

  9. Proteomic-based mechanistic investigation of low-dose radiation-induced cellular responses/effects

    SciTech Connect (OSTI)

    Chen, Xian

    2013-10-23

    The goal of our project is to apply our unique systems investigation strategy to reveal the molecular mechanisms underlying the radiation induction and transmission of oxidative damage, adaptive response, and bystander effect at low-doses. Beginning with simple in vitro systems such as fibroblast or epithelial pure culture, our amino acid-coded mass tagging (AACT) comparative proteomic platform will be used to measure quantitatively proteomic changes at high- or low-dose level with respect to their endogenous damage levels respectively, in which a broad range of unique regulated proteins sensitive to low-dose IR will be distinguished. To zoom in how these regulated proteins interact with other in the form of networks in induction/transmission pathways, these regulated proteins will be selected as baits for making a series of fibroblast cell lines that stably express each of them. Using our newly developed method of ?dual-tagging? quantitative proteomics that integrate the capabilities of natural complex expression/formation, simple epitope affinity isolation (not through tandem affinity purification or TAP), and ?in-spectra? AACT quantitative measurements using mass spectrometry (MS), we will be able to distinguish systematically interacting proteins with each bait in real time. Further, in addition to both proteome-wide (global differentially expressed proteins) and pathway-scale (bait-specific) profiling information, we will perform a computational network analysis to elucidate a global pathway/mechanisms underlying cellular responses to real-time low-dose IR. Similarly, we will extend our scheme to investigate systematically those induction/transmission pathways occurring in a fibroblast-epithelial interacting model in which the bystander cell (fibroblast) monitor the IR damage to the target cell (epithelial cell). The results will provide the proteome base (molecular mechanisms/pathways for signaling) for the low dose radiation-induced essential tissue environment interactions, including cell-cell, extracellular matrix and extracellular paracrine interactions.

  10. Fuel-Induced System Responses The Role Unconventional Fuels May...

    Broader source: Energy.gov (indexed) [DOE]

    Biodiesel's Enabling Characteristics in Attaining Low Temperature Diesel Combustion System-Response Issues Imposed by Biodiesel in a Medium-Duty Diesel Engine New Diesel Emissions ...

  11. Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials Citation Details In-Document ... Publication Date: 2014-07-08 OSTI Identifier: 1136695 Report ...

  12. Ultrafast terahertz-induced response of GeSbTe phase-change materials...

    Office of Scientific and Technical Information (OSTI)

    Title: Ultrafast terahertz-induced response of GeSbTe phase-change materials The time-reso... pulses as a means of biasing phase-change materials on a sub-picosecond time-scale. ...

  13. Laser speckle contrast imaging of skin blood perfusion responses induced by

    Office of Scientific and Technical Information (OSTI)

    laser coagulation (Journal Article) | SciTech Connect Laser speckle contrast imaging of skin blood perfusion responses induced by laser coagulation Citation Details In-Document Search Title: Laser speckle contrast imaging of skin blood perfusion responses induced by laser coagulation We report application of laser speckle contrast imaging (LSCI), i.e., a fast imaging technique utilising backscattered light to distinguish such moving objects as red blood cells from such stationary objects as

  14. Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Terahertz-Induced Response of GeSbTe Phase-Change Materials Citation Details In-Document Search Title: Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials Authors: Shu, Michael J. ; Zalden, Peter ; Chen, Frank ; Weems, Ben ; Chatzakis, Ioannis ; Xiong, Feng ; Jeyasingh, Rakesh ; Hoffmann, Matthias C. ; Pop, Eric ; Wong, H.-S.Philip ; Wuttig, Matthias ; Lindenberg, Aaron M. Publication Date: 2014-07-08 OSTI Identifier: 1136695 Report

  15. Ultrafast terahertz-induced response of GeSbTe phase-change materials

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect terahertz-induced response of GeSbTe phase-change materials Citation Details In-Document Search Title: Ultrafast terahertz-induced response of GeSbTe phase-change materials The time-resolved ultrafast electric field-driven response of crystalline and amorphous GeSbTe films has been measured all-optically, pumping with single-cycle terahertz pulses as a means of biasing phase-change materials on a sub-picosecond time-scale. Utilizing the near-band-gap

  16. Sirt2 suppresses inflammatory responses in collagen-induced arthritis

    SciTech Connect (OSTI)

    Lin, Jiangtao; Department of Orthopaedics, Yantaishan Hospital, 91 Jiefang Road, Yantai, Shandong 264001 ; Sun, Bing; Jiang, Chuanqiang; Hong, Huanyu; Zheng, Yanping

    2013-11-29

    Highlights: Sirt2 expression decreases in collagen-induced arthritis (CIA). Sirt2 knockout aggravates severity of arthritis in mice with CIA. Sirt2 knockout increases levels of pro-inflammatory factors in the serum. Sirt2 deacetylates p65 and inhibits pro-inflammatory factors expression. Sirt2 rescue abates severity of arthritis in mice with CIA. -- Abstract: Arthritis is a common autoimmune disease that is associated with progressive disability, systemic complications and early death. However, the underling mechanisms of arthritis are still unclear. Sirtuins are a NAD{sup +}-dependent class III deacetylase family, and regulate cellular stress, inflammation, genomic stability, carcinogenesis, and energy metabolism. Among the sirtuin family members, Sirt1 and Sirt6 are critically involved in the development of arthritis. It remains unknown whether other sirtuin family members participate in arthritis. Here in this study, we demonstrate that Sirt2 inhibits collagen-induced arthritis (CIA) using in vivo and in vitro evidence. The protein and mRNA levels of Sirt2 significantly decreased in joint tissues of mice with CIA. When immunized with collagen, Sirt2-KO mice showed aggravated severity of arthritis based on clinical scores, hind paw thickness, and radiological and molecular findings. Mechanically, Sirt2 deacetylated p65 subunit of nuclear factor-kappa B (NF-?B) at lysine 310, resulting in reduced expression of NF-?B-dependent genes, including interleukin 1? (IL-1?), IL-6, monocyte chemoattractant protein 1(MCP-1), RANTES, matrix metalloproteinase 9 (MMP-9) and MMP-13. Importantly, our rescue experiment showed that Sirt2 re-expression abated the severity of arthritis in Sirt2-KO mice. Those findings strongly indicate Sirt2 as a considerably inhibitor of the development of arthritis.

  17. Neurodegeneration and adaptation in response to low-dose photon irradiation

    SciTech Connect (OSTI)

    Limoli, Charles L.

    2014-10-27

    Neural stem and precursor cells (i.e. multipotent neural cells) are concentrated in the neurogenic regions of the brain (hippocampal dentate gyrus, subventricular zones), and considerable evidence suggests that these cells are important in mediating the stress response of the CNS after damage from ionizing radiation. The capability of these cells to proliferate, migrate and differentiate (i.e. to undergo neurogenesis) suggests they can participate in the repair and maintenance of CNS functions by replacing brain cells damaged or depleted due to irradiation. Importantly, we have shown that multipotent neural cells are markedly sensitive to irradiation and oxidative stress, insults that compromise neurogenesis and hasten the onset and progression of degenerative processes that are likely to have an adverse impact on cognition. Our past and current work has demonstrated that relatively low doses of radiation cause a persistent (weeks-months) oxidative stress in multipotent neural cells that can elicit a range of degenerative sequelae in the CNS. Therefore, our project is focused on determining the extent that endogenous and redox sensitive multipotent neural cells represent important radioresponsive targets for low dose radiation effects. We hypothesize that the activation of redox sensitive signaling can trigger radioadaptive changes in these cells that can be either harmful or beneficial to overall cognitive health.

  18. Final Report Systems Level Analysis of the Function and Adaptive Responses of Methanogenic Consortia

    SciTech Connect (OSTI)

    Lovley, Derek R.

    2015-03-09

    The purpose of this research was to determine whether the syntrophic microbial associations that are central to the functioning of methane-producing terrestrial wetlands can be predictively modeled with coupled multi-species genome-scale metabolic models. Such models are important because methane is an important greenhouse gas and there is a need to predictively model how the methane-producing microbial communities will respond to environmental perturbations, such as global climate change. The research discovered that the most prodigious methane-producing microorganisms on earth participate in a previously unrecognized form of energy exchange. The methane-producers Methanosaeta and Methanosarcina forge biological electrical connections with other microbes in order to obtain electrons to reduce carbon dioxide to methane. This direct interspecies electron transfer (DIET) was demonstrated in complex microbial communities as well as in defined co-cultures. For example, metatranscriptomic analysis of gene expression in both natural communities and defined co-cultures demonstrated that Methanosaeta species highly expressed genes for the enzymes for the reduction of carbon dioxide to methane. Furthermore, Methanosaetas electron-donating partners highly expressed genes for the biological electrical connections known as microbial nanowires. A series of studies involving transcriptomics, genome resequencing, and analysis of the metabolism of a series of strains with targeted gene deletions, further elucidated the mechanisms and energetics of DIET in methane-producing co-cultures, as well as in a co-culture of Geobacter metallireducens and Geobacter sulfurreducens, which provided a system for studying DIET with two genetically tractable partners. Genome-scale modeling of DIET in the G. metallireducens/G. sulfurreducens co-culture suggested that DIET provides more energy to the electron-donating partner that electron exchange via interspecies hydrogen transfer, but that the performance of DIET may be strongly influenced by environmental factors. These studies have significantly modified conceptual models for carbon and electron flow in methane-producing environments and have developed a computational framework for predictive modeling the influence of environmental perturbations on methane-producing microbial communities. The results have important implications for modeling the response of methane-producing microbial communities to climate change as well as for the bioenergy strategy of converting wastes and biomass to methane.

  19. Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Terahertz-Induced Response of GeSbTe Phase-Change Materials Citation Details In-Document Search Title: Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy science and

  20. Arsenic- and cadmium-induced toxicogenomic response in mouse embryos undergoing neurulation

    SciTech Connect (OSTI)

    Robinson, Joshua F.; Yu, Xiaozhong; Moreira, Estefania G.; Hong, Sungwoo; Faustman, Elaine M.

    2011-01-15

    Arsenic (As) and cadmium (Cd) are well-characterized teratogens in animal models inducing embryotoxicity and neural tube defects (NTDs) when exposed during neurulation. Toxicological research is needed to resolve the specific biological processes and associated molecular pathways underlying metal-induced toxicity during this timeframe in gestational development. In this study, we investigated the dose-dependent effects of As and Cd on gene expression in C57BL/6J mouse embryos exposed in utero during neurulation (GD8) to identify significantly altered genes and corresponding biological processes associated with embryotoxicity. We quantitatively examined the toxicogenomic dose-response relationship at the gene level. Our results suggest that As and Cd induce dose-dependent gene expression alterations representing shared (cell cycle, response to UV, glutathione metabolism, RNA processing) and unique (alcohol/sugar metabolism) biological processes, which serve as robust indicators of metal-induced developmental toxicity and indicate underlying embryotoxic effects. Our observations also correlate well with previously identified impacts of As and Cd on specific genes associated with metal-induced toxicity (Cdkn1a, Mt1). In summary, we have identified in a quantitative manner As and Cd induced dose-dependent effects on gene expression in mouse embryos during a peak window of sensitivity to embryotoxicity and NTDs in the sensitive C57BL/6J strain.

  1. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  2. Hydrogen-peroxide-induced oxidative stress responses in Desulfovibrio vulgaris Hildenborough

    SciTech Connect (OSTI)

    Zhou, A.; He, Z.; Redding-Johanson, A.M.; Mukhopadhyay, A.; Hemme, C.L.; Joachimiak, M.P.; Bender, K.S.; Keasling, J.D.; Stahl, D.A.; Fields, M.W.; Hazen, T.C.; Arkin, A.P.; Wall, J.D.; Zhou, J.; Luo, F.; Deng, Y.; He, Q.

    2010-07-01

    To understand how sulphate-reducing bacteria respond to oxidative stresses, the responses of Desulfovibrio vulgaris Hildenborough to H{sub 2}O{sub 2}-induced stresses were investigated with transcriptomic, proteomic and genetic approaches. H{sub 2}O{sub 2} and induced chemical species (e.g. polysulfide, ROS) and redox potential shift increased the expressions of the genes involved in detoxification, thioredoxin-dependent reduction system, protein and DNA repair, and decreased those involved in sulfate reduction, lactate oxidation and protein synthesis. A gene coexpression network analysis revealed complicated network interactions among differentially expressed genes, and suggested possible importance of several hypothetical genes in H{sub 2}O{sub 2} stress. Also, most of the genes in PerR and Fur regulons were highly induced, and the abundance of a Fur regulon protein increased. Mutant analysis suggested that PerR and Fur are functionally overlapped in response to stresses induced by H{sub 2}O{sub 2} and reaction products, and the upregulation of thioredoxin-dependent reduction genes was independent of PerR or Fur. It appears that induction of those stress response genes could contribute to the increased resistance of deletion mutants to H{sub 2}O{sub 2}-induced stresses. In addition, a conceptual cellular model of D. vulgaris responses to H{sub 2}O{sub 2} stress was constructed to illustrate that this bacterium may employ a complicated molecular mechanism to defend against the H{sub 2}O{sub 2}-induced stresses.

  3. Adaptive sampler

    DOE Patents [OSTI]

    Watson, Bobby L.; Aeby, Ian

    1982-01-01

    An adaptive data compression device for compressing data having variable frequency content, including a plurality of digital filters for analyzing the content of the data over a plurality of frequency regions, a memory, and a control logic circuit for generating a variable rate memory clock corresponding to the analyzed frequency content of the data in the frequency region and for clocking the data into the memory in response to the variable rate memory clock.

  4. Adaptive sampler

    DOE Patents [OSTI]

    Watson, B.L.; Aeby, I.

    1980-08-26

    An adaptive data compression device for compressing data is described. The device has a frequency content, including a plurality of digital filters for analyzing the content of the data over a plurality of frequency regions, a memory, and a control logic circuit for generating a variable rate memory clock corresponding to the analyzed frequency content of the data in the frequency region and for clocking the data into the memory in response to the variable rate memory clock.

  5. Diet-Induced Obesity Reprograms the Inflammatory Response of the Murine Lung to Inhaled Endotoxin

    SciTech Connect (OSTI)

    Tilton, Susan C.; Waters, Katrina M.; Karin, Norman J.; Webb-Robertson, Bobbie-Jo M.; Zangar, Richard C.; Lee, Monika K.; Bigelow, Diana J.; Pounds, Joel G.; Corley, Richard A.

    2013-03-01

    The co-occurrence of environmental factors is common in complex human diseases and, as such, understanding the molecular responses involved is essential to determine risk and susceptibility to disease. We have investigated the key biological pathways that define susceptibility for pulmonary infection during obesity in diet-induced obese (DIO) and regular weight (RW) C57BL/6 mice exposed to inhaled lipopolysaccharide (LPS). LPS induced a strong inflammatory response in all mice as indicated by elevated cell counts of macrophages and neutrophils and levels of proinflammatory cytokines (MDC, MIP-1?, IL-12, RANTES) in the bronchoalveolar lavage fluid. Additionally, DIO mice exhibited 50% greater macrophage cell counts, but decreased levels of the cytokines, IL-6, TARC, TNF-?, and VEGF relative to RW mice. Microarray analysis of lung tissue showed over half of the LPS-induced expression in DIO mice consisted of genes unique for obese mice, suggesting that obesity reprograms how the lung responds to subsequent insult. In particular, we found that obese animals exposed to LPS have gene signatures showing increased inflammatory and oxidative stress response and decreased antioxidant capacity compared with RW. Because signaling pathways for these responses can be common to various sources of environmentally induced lung damage, we further identified biomarkers that are indicative of specific toxicant exposure by comparing gene signatures after LPS exposure to those from a parallel study with cigarette smoke. These data show obesity may increase sensitivity to further insult and that co-occurrence of environmental stressors result in complex biosignatures that are not predicted from analysis of individual exposures.

  6. Diet-induced obesity reprograms the inflammatory response of the murine lung to inhaled endotoxin

    SciTech Connect (OSTI)

    Tilton, Susan C.; Waters, Katrina M.; Karin, Norman J.; Webb-Robertson, Bobbie-Jo M.; Zangar, Richard C.; Lee, K. Monica; Bigelow, Diana J.; Pounds, Joel G.; Corley, Richard A.

    2013-03-01

    The co-occurrence of environmental factors is common in complex human diseases and, as such, understanding the molecular responses involved is essential to determine risk and susceptibility to disease. We have investigated the key biological pathways that define susceptibility for pulmonary infection during obesity in diet-induced obese (DIO) and regular weight (RW) C57BL/6 mice exposed to inhaled lipopolysaccharide (LPS). LPS induced a strong inflammatory response in all mice as indicated by elevated cell counts of macrophages and neutrophils and levels of proinflammatory cytokines (MDC, MIP-1?, IL-12, RANTES) in the bronchoalveolar lavage fluid. Additionally, DIO mice exhibited 50% greater macrophage cell counts, but decreased levels of the cytokines, IL-6, TARC, TNF-?, and VEGF relative to RW mice. Microarray analysis of lung tissue showed over half of the LPS-induced expression in DIO mice consisted of genes unique for obese mice, suggesting that obesity reprograms how the lung responds to subsequent insult. In particular, we found that obese animals exposed to LPS have gene signatures showing increased inflammatory and oxidative stress response and decreased antioxidant capacity compared with RW. Because signaling pathways for these responses can be common to various sources of environmentally induced lung damage, we further identified biomarkers that are indicative of specific toxicant exposure by comparing gene signatures after LPS exposure to those from a parallel study with cigarette smoke. These data show obesity may increase sensitivity to further insult and that co-occurrence of environmental stressors result in complex biosignatures that are not predicted from analysis of individual exposures. - Highlights: ? Obesity modulates inflammatory markers in BAL fluid after LPS exposure. ? Obese animals have a unique transcriptional signature in lung after LPS exposure. ? Obesity elevates inflammatory stress and reduces antioxidant capacity in the lung. ? Toxicant-specific biomarkers predict exposure independent of systemic inflammation.

  7. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish

    SciTech Connect (OSTI)

    Goodale, Britton C.; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn R.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GCMS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. - Highlights: Defined global mRNA expression changes induced by developmental exposure to PAHs Determined PAH body burdens following developmental exposure Genes uniquely induced by benz(a)anthracene included targets of the AHR and RELA Dibenzothiophene and pyrene perturbed a distinct RELA network Transcriptional networks reveal differential mechanisms of PAH toxicity.

  8. Time course of systemic oxidative stress and inflammatory response induced by an acute exposure to Residual Oil Fly Ash

    SciTech Connect (OSTI)

    Marchini, T.; Magnani, N.D.; Paz, M.L.; Vanasco, V.; Tasat, D.; Gonzlez Maglio, D.H.; and others

    2014-01-15

    It is suggested that systemic oxidative stress and inflammation play a central role in the onset and progression of cardiovascular diseases associated with the exposure to particulate matter (PM). The aim of this work was to evaluate the time changes of systemic markers of oxidative stress and inflammation, after an acute exposure to Residual Oil Fly Ash (ROFA). Female Swiss mice were intranasally instilled with a ROFA suspension (1.0 mg/kg body weight) or saline solution, and plasma levels of oxidative damage markers [thiobarbituric acid reactive substances (TBARSs) and protein carbonyls], antioxidant status [reduced (GSH) and oxidized (GSSG) glutathione, ascorbic acid levels, and superoxide dismutase (SOD) activity], cytokines levels, and intravascular leukocyte activation were evaluated after 1, 3 or 5 h of exposure. Oxidative damage to lipids and decreased GSH/GSSG ratio were observed in ROFA-exposed mice as early as 1 h. Afterwards, increased protein oxidation, decreased ascorbic acid content and SOD activity were found in this group at 3 h. The onset of an adaptive response was observed at 5 h after the ROFA exposure, as indicated by decreased TBARS plasma content and increased SOD activity. The observed increase in oxidative damage to plasma macromolecules, together with systemic antioxidants depletion, may be a consequence of a systemic inflammatory response triggered by the ROFA exposure, since increased TNF-? and IL-6 plasma levels and polymorphonuclear leukocytes activation was found at every evaluated time point. These findings contribute to the understanding of the increase in cardiovascular morbidity and mortality, in association with environmental PM inhalation. - Highlights: An acute exposure to ROFA triggers the occurrence of systemic oxidative stress. Changes in plasmatic oxidative stress markers appear as early as 1 h after exposure. ROFA induces proinflammatory cytokines release and intravascular leukocyte activation. PMN activation is a relevant source of reactive oxygen species in this model. These findings may account for previously described cardiopulmonary alterations.

  9. Ultrafast terahertz-induced response of GeSbTe phase-change materials

    SciTech Connect (OSTI)

    Shu, Michael J.; Zalden, Peter; Chen, Frank; Weems, Ben; Chatzakis, Ioannis; Xiong, Feng; Jeyasingh, Rakesh; Pop, Eric; Philip Wong, H.-S.; Hoffmann, Matthias C.; Wuttig, Matthias; Lindenberg, Aaron M.

    2014-06-23

    The time-resolved ultrafast electric field-driven response of crystalline and amorphous GeSbTe films has been measured all-optically, pumping with single-cycle terahertz pulses as a means of biasing phase-change materials on a sub-picosecond time-scale. Utilizing the near-band-gap transmission as a probe of the electronic and structural response below the switching threshold, we observe a field-induced heating of the carrier system and resolve the picosecond-time-scale energy relaxation processes and their dependence on the sample annealing condition in the crystalline phase. In the amorphous phase, an instantaneous electroabsorption response is observed, quadratic in the terahertz field, followed by field-driven lattice heating, with Ohmic behavior up to 200 kV/cm.

  10. Global protein phosphorylation dynamics during deoxynivalenol-induced ribotoxic stress response in the macrophage

    SciTech Connect (OSTI)

    Pan, Xiao; Whitten, Douglas A.; Wu, Ming; Chan, Christina; Wilkerson, Curtis G.; Pestka, James J.

    2013-04-15

    Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium that commonly contaminates food, is capable of activating mononuclear phagocytes of the innate immune system via a process termed the ribotoxic stress response (RSR). To encapture global signaling events mediating RSR, we quantified the early temporal (≤ 30 min) phosphoproteome changes that occurred in RAW 264.7 murine macrophage during exposure to a toxicologically relevant concentration of DON (250 ng/mL). Large-scale phosphoproteomic analysis employing stable isotope labeling of amino acids in cell culture (SILAC) in conjunction with titanium dioxide chromatography revealed that DON significantly upregulated or downregulated phosphorylation of 188 proteins at both known and yet-to-be functionally characterized phosphosites. DON-induced RSR is extremely complex and goes far beyond its prior known capacity to inhibit translation and activate MAPKs. Transcriptional regulation was the main target during early DON-induced RSR, covering over 20% of the altered phosphoproteins as indicated by Gene Ontology annotation and including transcription factors/cofactors and epigenetic modulators. Other biological processes impacted included cell cycle, RNA processing, translation, ribosome biogenesis, monocyte differentiation and cytoskeleton organization. Some of these processes could be mediated by signaling networks involving MAPK-, NFκB-, AKT- and AMPK-linked pathways. Fuzzy c-means clustering revealed that DON-regulated phosphosites could be discretely classified with regard to the kinetics of phosphorylation/dephosphorylation. The cellular response networks identified provide a template for further exploration of the mechanisms of trichothecenemycotoxins and other ribotoxins, and ultimately, could contribute to improved mechanism-based human health risk assessment. - Highlights: ► Mycotoxin deoxynivalenol (DON) induces immunotoxicity via ribotoxic stress response. ► SILAC phosphoproteomics using TiO{sub 2} was applied to DON-treated RAW 264.7 cells. ► DON induces extensive protein phosphorylation changes involving 188 phosphoproteins. ► The main target of early DON-induced RSR is transcriptional regulation. ► Early DON-induced RSR is mediated by MAPK-, NFκB-, AKT- and AMPK-linked pathways.

  11. Continuum resonance induced electromagnetic torque by a rotating plasma response to static resonant magnetic perturbation field

    SciTech Connect (OSTI)

    Liu Yueqiang; Connor, J. W.; Cowley, S. C.; Ham, C. J.; Hastie, R. J.; Hender, T. C.

    2012-10-15

    A numerical study is carried out, based on a simple toroidal tokamak equilibrium, to demonstrate the radial re-distribution of the electromagnetic torque density, as a result of a rotating resistive plasma (linear) response to a static resonant magnetic perturbation field. The computed electromagnetic torque peaks at several radial locations even in the presence of a single rational surface, due to resonances between the rotating response, in the plasma frame, and both Alfven and sound continuum waves. These peaks tend to merge together to form a rather global torque distribution, when the plasma resistivity is large. The continuum resonance induced net electromagnetic torque remains finite even in the limit of an ideal plasma.

  12. Sulfur mustard induces an endoplasmic reticulum stress response in the mouse ear vesicant model

    SciTech Connect (OSTI)

    Chang, Yoke-Chen; Wang, James D.; Svoboda, Kathy K.; Casillas, Robert P.; Laskin, Jeffrey D.; Gordon, Marion K.; Gerecke, Donald R.

    2013-04-15

    The endoplasmic reticulum (ER) stress response is a cell survival pathway upregulated when cells are under severe stress. Severely damaged mouse ear skin exposed to the vesicant, sulfur mustard (bis-2-chloroethyl sulfide, SM), resulted in increased expression of ER chaperone proteins that accompany misfolded and incorrectly made proteins targeted for degradation. Time course studies with SM using the mouse ear vesicant model (MEVM) showed progressive histopathologic changes including edema, separation of the epidermis from the dermis, persistent inflammation, upregulation of laminin ?2 (one of the chains of laminin-332, a heterotrimeric skin glycoprotein required for wound repair), and delayed wound healing from 24 h to 168 h post exposure. This was associated with time related increased expression of the cell survival ER stress marker, GRP78/BiP, and the ER stress apoptosis marker, GADD153/CHOP, suggesting simultaneous activation of both cell survival and non-mitochondrial apoptosis pathways. Dual immunofluorescence labeling of a keratinocyte migration promoting protein, laminin ?2 and GRP78/BIP, showed colocalization of the two molecules 72 h post exposure indicating that the laminin ?2 was misfolded after SM exposure and trapped within the ER. Taken together, these data show that ER stress is induced in mouse skin within 24 h of vesicant exposure in a defensive response to promote cell survival; however, it appears that this response is rapidly overwhelmed by the apoptotic pathway as a consequence of severe SM-induced injury. - Highlights: ? We demonstrated ER stress response in the mouse ear vesicant model. ? We described the asymmetrical nature of wound repair in the MEVM. ? We identified the distribution of various ER stress markers in the MEVM.

  13. Experimental characterization of fire-induced response of rigid polyurethane foam

    SciTech Connect (OSTI)

    Chu, T.Y.; Gill, W.; Moore, J.W.; Hobbs, M.L.; Gritzo, L.A.; Moya, J.L.

    1995-12-31

    Reported is the result of an experimental investigation of fire-induced response of a 96 kg/m{sup 3} closed cell rigid polyurethane foam. The specimen is 0.37 m in diameter, and 152 mm thick, placed in a cylindrical test vessel. The fire condition is simulated by heating the bottom of the test vessel to 1283 K using a radiant heat source. Real-time x-ray shows that the degradation process involves the progression of a charring front into the virgin material. The charred region has a regular and graded structure consisting of a packed bubble outer layer and successive layers of thin shells. The layer-to-layer permeability appears to be poor. There are indications that gas vents laterally. The shell-like structure might be the result of lateral venting. Although the foam degradation process is quite complicated, the in-depth temperature responses in the uncharted foam appear to be consistent with steady state ablation. The measured temperature responses are well represented by the exponential distribution for steady state ablation. An estimate of the thermal diffusivity of the foam is obtained from the ablation model. The experiment is part of a more comprehensive program to develop material response models of foams and encapsulants.

  14. Mono-2-ethylhexyl phthalate induces oxidative stress responses in human placental cells in vitro

    SciTech Connect (OSTI)

    Tetz, Lauren M.; Cheng, Adrienne A.; Korte, Cassandra S.; Giese, Roger W.; Wang, Poguang; Harris, Craig; Meeker, John D.; Loch-Caruso, Rita

    2013-04-01

    Di-2-ethylhexyl phthalate (DEHP) is an environmental contaminant commonly used as a plasticizer in polyvinyl chloride products. Exposure to DEHP has been linked to adverse pregnancy outcomes in humans including preterm birth, low birth-weight, and pregnancy loss. Although oxidative stress is linked to the pathology of adverse pregnancy outcomes, effects of DEHP metabolites, including the active metabolite, mono-2-ethylhexyl phthalate (MEHP), on oxidative stress responses in placental cells have not been previously evaluated. The objective of the current study is to identify MEHP-stimulated oxidative stress responses in human placental cells. We treated a human placental cell line, HTR-8/SVneo, with MEHP and then measured reactive oxygen species (ROS) generation using the dichlorofluorescein assay, oxidized thymine with mass-spectrometry, redox-sensitive gene expression with qRT-PCR, and apoptosis using a luminescence assay for caspase 3/7 activity. Treatment of HTR-8 cells with 180 ?M MEHP increased ROS generation, oxidative DNA damage, and caspase 3/7 activity, and resulted in differential expression of redox-sensitive genes. Notably, 90 and 180 ?M MEHP significantly induced mRNA expression of prostaglandin-endoperoxide synthase 2 (PTGS2), an enzyme important for synthesis of prostaglandins implicated in initiation of labor. The results from the present study are the first to demonstrate that MEHP stimulates oxidative stress responses in placental cells. Furthermore, the MEHP concentrations used were within an order of magnitude of the highest concentrations measured previously in human umbilical cord or maternal serum. The findings from the current study warrant future mechanistic studies of oxidative stress, apoptosis, and prostaglandins as molecular mediators of DEHP/MEHP-associated adverse pregnancy outcomes. - Highlights: ? MEHP increased reactive oxygen species, oxidative DNA damage, and caspase activity. ? MEHP induced expression of PTGS2, a gene important in pregnancy and parturition ? MEHP treatment resulted in differential expression of GLRX2, TXNRD1, and DHCR24.

  15. Crucial role of Toll-like receptors in the zinc/nickel-induced inflammatory response in vascular endothelial cells

    SciTech Connect (OSTI)

    Tsou, Tsui-Chun; Liou, Saou-Hsing; Yeh, Szu-Ching; Tsai, Feng-Yuan; Chao, How-Ran

    2013-12-15

    Our previous studies indicated that zinc induced inflammatory response in both vascular endothelial cells and promonocytes. Here, we asked if other metals could cause the similar effect on vascular endothelial cells and tried to determine its underlying mechanism. Following screening of fifteen metals, zinc and nickel were identified with a marked proinflammatory effect, as determined by ICAM-1 and IL-8 induction, on human umbilical vein endothelial cells (HUVECs). Inhibiting protein expression of myeloid differentiation primary response protein-88 (MyD88), a Toll-like receptor (TLR) adaptor acting as a TLR-signaling transducer, significantly attenuated the zinc/nickel-induced inflammatory response, suggesting the critical roles of TLRs in the inflammatory response. Blockage of TLR-4 signaling by CLI-095, a TLR-4 inhibitor, completely inhibited the nickel-induced ICAM-1 and IL-8 expression and NF?B activation. The same CLI-095 treatment significantly blocked the zinc-induced IL-8 expression, however with no significant effect on the ICAM-1 expression and a minor inhibitory effect on the NF?B activation. The finding demonstrated the differential role of TLR-4 in regulation of the zinc/nickel-induced inflammatory response, where TLR-4 played a dominant role in NF?B activation by nickel, but not by zinc. Moreover, inhibition of NF?B by adenovirus-mediated I?B? expression and Bay 11-7025, an inhibitor of cytokine-induced I?B-? phosphorylation, significantly attenuated the zinc/nickel-induced inflammatory responses, indicating the critical of NF?B in the process. The study demonstrates the crucial role of TLRs in the zinc/nickel-induced inflammatory response in vascular endothelial cells and herein deciphers a potential important difference in NF?B activation via TLRs. The study provides a molecular basis for linkage between zinc/nickel exposure and pathogenesis of the metal-related inflammatory vascular disease. - Highlights: Both zinc and nickel cause ICAM-1/IL?8 expression in endothelial cells via TLRs. Nickel induces the inflammatory responses via a TLR-4/NF-?B pathway. Zinc causes the inflammatory responses via a broader TLRs/NF-?B signaling. Nickel shows a significantly higher inflammatory effect than zinc. NF-?B activation is the primary mechanism involved in the inflammatory responses.

  16. Adapting the U.S. Domestic Radiological Emergency Response Process to an Overseas Incident: FRMAC Without the F

    SciTech Connect (OSTI)

    Blumenthal, Daniel J.; Bowman, David R.; Remick, Alan

    2012-05-01

    The earthquake and resulting tsunami in Japan led to a radiological release from the Fukushima Daiichi Nuclear Power Plan, which in turn resulted in the rapid activation and deployment by the U.S. Department of Energy National Nuclear Security Administration (DOE/NNSA) emergency response teams. These teams and those from other federal agencies are typically coordinated through the Federal Radiological Monitoring and Assessment Center (FRMAC) when responding to radiological incidents in the U.S. FRMAC is the body through which the collection, analysis, and assessment of environmental radiological data are coordinated and products released to decision makers. This article discusses DOE/NNSAs role in the U.S. response to the Fukushima accident as it implemented its components of FRMAC in a foreign country, coordinated its assets, integrated with its federal partners, and collaborated with the Government of Japan. The technical details of the various data collections and analyses are covered in other articles of this issue.

  17. Geomechanical response of permafrost-associated hydrate deposits to depressurization-induced gas production

    SciTech Connect (OSTI)

    Rutqvist, J.; Moridis, G.J.; Grover, T.; Collett, T.

    2009-02-01

    In this simulation study, we analyzed the geomechanical response during depressurization production from two known hydrate-bearing permafrost deposits: the Mallik (Northwest Territories, Canada) deposit and Mount Elbert (Alaska, USA) deposit. Gas was produced from these deposits at constant pressure using horizontal wells placed at the top of a hydrate layer (HL), located at a depth of about 900 m at the Mallik and 600 m at the Mount Elbert. The simulation results show that general thermodynamic and geomechanical responses are similar for the two sites, but with substantially higher production and more intensive geomechanical responses at the deeper Mallik deposit. The depressurization-induced dissociation begins at the well bore and then spreads laterally, mainly along the top of the HL. The depressurization results in an increased shear stress within the body of the receding hydrate and causes a vertical compaction of the reservoir. However, its effects are partially mitigated by the relatively stiff permafrost overburden, and compaction of the HL is limited to less than 0.4%. The increased shear stress may lead to shear failure in the hydrate-free zone bounded by the HL overburden and the downward-receding upper dissociation interface. This zone undergoes complete hydrate dissociation, and the cohesive strength of the sediment is low. We determined that the likelihood of shear failure depends on the initial stress state as well as on the geomechanical properties of the reservoir. The Poisson's ratio of the hydrate-bearing formation is a particularly important parameter that determines whether the evolution of the reservoir stresses will increase or decrease the likelihood of shear failure.

  18. Non-dioxin-like PCBs interact with benzo[a]pyrene-induced p53-responses and inhibit apoptosis

    SciTech Connect (OSTI)

    Al-Anati, Lauy Hoegberg, Johan; Stenius, Ulla

    2010-12-01

    Non-dioxin-like polychlorinated biphenyls (NDL-PCBs) and polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants often co-existing in contaminated environments. However, there are few studies on the effects of co-exposure, in particular on effects of pure NDL-PCB congeners and PAHs. We have evaluated the effects of some highly purified NDL-PCBs and benzo[a]pyrene (BP) on BP-induced Raf, Erk, Mdm2, p53 signaling and on BP-induced apoptosis and cell cycle arrest. PCBs (1 {mu}M) were added to HepG2 cells 1 h prior to BP and the incubation was stopped at 24 h. Employing Western blotting we found that NDL-PCBs (28, 101 and 153) amplified the BP-induced inactivating phosphorylation of Raf (pRaf Ser 259) and decreased levels of pErk Tyr 204. This treatment also resulted in the attenuation of BP-induced Mdm2 phosphorylation at Ser166 and amplification of the p53 Ser15 response. These effects were associated with an unexpected inhibition of BP-induced apoptosis. A dioxin-like PCB (DL-PCB 126) was used as reference and gave results that were predictable from previous studies, i.e. it attenuated BP-induced p53 response and apoptosis. In an effort to explain why the NDL-PCB-induced amplification of the p53 response was associated with a decreased apoptotic response we analyzed FoxO3a, which may translocate p53 to the cytoplasm. We found that NDL-PCBs reduced the level of phosphorylated FoxO3a at Thr32. This phosphorylation promotes a cytoplasmic translocation of FoxO3a and p53 and our data suggest that NDL-PCBs may inhibit BP-induced apoptosis by preventing a FoxO3a-dependent translocation of p53 to the cytoplasm.

  19. WRNIP1 functions upstream of DNA polymerase ? in the UV-induced DNA damage response

    SciTech Connect (OSTI)

    Yoshimura, Akari; Kobayashi, Yume; Tada, Shusuke; Seki, Masayuki; Enomoto, Takemi

    2014-09-12

    Highlights: The UV sensitivity of POLH{sup ?/?} cells was suppressed by disruption of WRNIP1. In WRNIP1{sup ?/?/?}/POLH{sup ?/?} cells, mutation frequencies and SCE after irradiation reduced. WRNIP1 defect recovered rate of fork progression after irradiation in POLH{sup ?/?} cells. WRNIP1 functions upstream of Pol? in the translesion DNA synthesis pathway. - Abstract: WRNIP1 (WRN-interacting protein 1) was first identified as a factor that interacts with WRN, the protein that is defective in Werner syndrome (WS). WRNIP1 associates with DNA polymerase ? (Pol?), but the biological significance of this interaction remains unknown. In this study, we analyzed the functional interaction between WRNIP1 and Pol? by generating knockouts of both genes in DT40 chicken cells. Disruption of WRNIP1 in Pol?-disrupted (POLH{sup ?/?}) cells suppressed the phenotypes associated with the loss of Pol?: sensitivity to ultraviolet light (UV), delayed repair of cyclobutane pyrimidine dimers (CPD), elevated frequency of mutation, elevated levels of UV-induced sister chromatid exchange (SCE), and reduced rate of fork progression after UV irradiation. These results suggest that WRNIP1 functions upstream of Pol? in the response to UV irradiation.

  20. The field induced e{sub 31,f} piezoelectric and Rayleigh response in barium strontium titanate thin films

    SciTech Connect (OSTI)

    Garten, L. M. Trolier-McKinstry, S.

    2014-09-29

    The electric field induced e{sub 31,f} piezoelectric response and tunability of Ba{sub 0.7}Sr{sub 0.3}TiO{sub 3} (70:30) and Ba{sub 0.6}Sr{sub 0.4}TiO{sub 3} (60:40) thin films on MgO and silicon was measured. The relative dielectric tunabilities for the 70:30 and 60:40 compositions on MgO were 83% and 70%, respectively, with a dielectric loss of less than 0.011 and 0.004 at 100 kHz. A linear increase in induced piezoelectricity to −3.0 C/m{sup 2} and −1.5 C/m{sup 2} at 110 kV/cm was observed in Ba{sub 0.6}Sr{sub 0.4}TiO{sub 3} on MgO and Ba{sub 0.7}Sr{sub 0.3}TiO{sub 3} on Si. Hysteresis in the piezoelectric and dielectric response of the 70:30 composition films was consistent with the positive irreversible dielectric Rayleigh coefficient. Both indicate a ferroelectric contribution to the piezoelectric and dielectric response over 40–80 °C above the global paraelectric transition temperature.

  1. COMMENTARY:Limits to adaptation

    SciTech Connect (OSTI)

    Preston, Benjamin L

    2013-01-01

    An actor-centered, risk-based approach to defining limits to social adaptation provides a useful analytic framing for identifying and anticipating these limits and informing debates over society s responses to climate change.

  2. Response Response

    National Nuclear Security Administration (NNSA)

    Attachment 7 Response Response Response Response Response Response Response Response Response Response Response Response Percent of Mentors that are People with Disabilities 9.00% Total number of Mentors (The count used to calculate the Mentor percentages) 252 Demographic Information Percent of Mentors Two or More Races Not reported Percent of White Mentors 63.00% Percent of Female Mentors 39.00% Percent of Male Mentors 61.00% Percent of Veteran Mentors 21.00% Percent of Asian American Mentors

  3. Adaptive Thresholds

    Energy Science and Technology Software Center (OSTI)

    2014-08-26

    ADAPT is a topological analysis code that allow to compute local threshold, in particular relevance based thresholds for features defined in scalar fields. The initial target application is vortex detection but the software is more generally applicable to all threshold based feature definitions.

  4. Adaptive sequential controller

    DOE Patents [OSTI]

    El-Sharkawi, Mohamed A.; Xing, Jian; Butler, Nicholas G.; Rodriguez, Alonso

    1994-01-01

    An adaptive sequential controller (50/50') for controlling a circuit breaker (52) or other switching device to substantially eliminate transients on a distribution line caused by closing and opening the circuit breaker. The device adaptively compensates for changes in the response time of the circuit breaker due to aging and environmental effects. A potential transformer (70) provides a reference signal corresponding to the zero crossing of the voltage waveform, and a phase shift comparator circuit (96) compares the reference signal to the time at which any transient was produced when the circuit breaker closed, producing a signal indicative of the adaptive adjustment that should be made. Similarly, in controlling the opening of the circuit breaker, a current transformer (88) provides a reference signal that is compared against the time at which any transient is detected when the circuit breaker last opened. An adaptive adjustment circuit (102) produces a compensation time that is appropriately modified to account for changes in the circuit breaker response, including the effect of ambient conditions and aging. When next opened or closed, the circuit breaker is activated at an appropriately compensated time, so that it closes when the voltage crosses zero and opens when the current crosses zero, minimizing any transients on the distribution line. Phase angle can be used to control the opening of the circuit breaker relative to the reference signal provided by the potential transformer.

  5. Nanostructure induced changes in lifetime and enhanced second-harmonic response of organic-plasmonic hybrids

    SciTech Connect (OSTI)

    Leißner, Till; Kostiučenko, Oksana; Rubahn, Horst-Günter; Fiutowski, Jacek; Brewer, Jonathan R.

    2015-12-21

    In this letter we show that the optical response of organic nanofibers, grown from functionalized para-quaterphenylene molecules, can be controlled by forming organic-plasmonic hybrid systems. The interaction between nanofibers and supporting regular arrays of nanostructures leads to a strongly enhanced second harmonic response. At the same time, the fluorescence lifetime of the nanofibers is reduced from 0.32 ns for unstructured gold films to 0.22 ns for gold nanosquare arrays, demonstrating efficient organic–plasmonic interaction. To study the origin of these effects, we applied two-photon laser scanning microscopy and fluorescence lifetime imaging microscopy. These findings provide an effective approach for plasmon-enhanced second-harmonic generation at the nanoscale, which is attractive for nanophotonic circuitry.

  6. Hepatic injury induces contrasting response in liver and kidney to chemicals that are metabolically activated: Role of male sex hormone

    SciTech Connect (OSTI)

    Kim, Young C. [College of Pharmacy, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul (Korea, Republic of)], E-mail: youckim@snu.ac.kr; Yim, Hye K.; Jung, Young S. [College of Pharmacy, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul (Korea, Republic of); Park, Jae H. [College of Veterinary Medicine, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul (Korea, Republic of); Kim, Sung Y. [College of Pharmacy, Wonkwang University, 344-2 Shinyong-Dong, Iksan (Korea, Republic of)

    2007-08-15

    Injury to liver, resulting in loss of its normal physiological/biochemical functions, may adversely affect a secondary organ. We examined the response of the liver and kidney to chemical substances that require metabolic activation for their toxicities in mice with a preceding liver injury. Carbon tetrachloride treatment 24 h prior to a challenging dose of carbon tetrachloride or acetaminophen decreased the resulting hepatotoxicity both in male and female mice as determined by histopathological examination and increases in serum enzyme activities. In contrast, the renal toxicity of the challenging toxicants was elevated markedly in male, but not in female mice. Partial hepatectomy also induced similar changes in the hepatotoxicity and nephrotoxicity of a challenging toxicant, suggesting that the contrasting response of male liver and kidney was associated with the reduction of the hepatic metabolizing capacity. Carbon tetrachloride pretreatment or partial hepatectomy decreased the hepatic xenobiotic-metabolizing enzyme activities in both sexes but elevated the renal p-nitrophenol hydroxylase, p-nitroanisole O-demethylase and aminopyrine N-demethylase activities significantly only in male mice. Increases in Cyp2e1 and Cyp2b expression were also evident in male kidney. Castration of males or testosterone administration to females diminished the sex-related differences in the renal response to an acute liver injury. The results indicate that reduction of the hepatic metabolizing capacity induced by liver injury may render secondary target organs susceptible to chemical substances activated in these organs. This effect may be sex-specific. It is also suggested that an integrated approach should be taken for proper assessment of chemical hazards.

  7. Time-Resolved Imaging of Material Response Following Laser-Induced Breakdown in the Bulk and Surface of Fused Silica

    SciTech Connect (OSTI)

    Raman, R N; Negres, R A; DeMange, P; Demos, S G

    2010-02-04

    Optical components within high energy laser systems are susceptible to laser-induced material modification when the breakdown threshold is exceeded or damage is initiated by pre-existing impurities or defects. These modifications are the result of exposure to extreme conditions involving the generation of high temperatures and pressures and occur on a volumetric scale of the order of a few cubic microns. The response of the material following localized energy deposition, including the timeline of events and the individual processes involved during this timeline, is still largely unknown. In this work, we investigate the events taking place during the entire timeline in both bulk and surface damage in fused silica using a set of time-resolved microscopy systems. These microscope systems offer up to 1 micron spatial resolution when imaging static or dynamic effects, allowing for imaging of the entire process with adequate temporal and spatial resolution. These systems incorporate various pump-probe geometries designed to optimize the sensitivity for detecting individual aspects of the process such as the propagation of shock waves, near-surface material motion, the speed of ejecta, and material transformations. The experimental results indicate that the material response can be separated into distinct phases, some terminating within a few tens of nanoseconds but some extending up to about 100 microseconds. Overall the results demonstrate that the final characteristics of the modified region depend on the material response to the energy deposition and not on the laser parameters.

  8. Adaptive method with intercessory feedback control for an intelligent agent

    DOE Patents [OSTI]

    Goldsmith, Steven Y.

    2004-06-22

    An adaptive architecture method with feedback control for an intelligent agent provides for adaptively integrating reflexive and deliberative responses to a stimulus according to a goal. An adaptive architecture method with feedback control for multiple intelligent agents provides for coordinating and adaptively integrating reflexive and deliberative responses to a stimulus according to a goal. Re-programming of the adaptive architecture is through a nexus which coordinates reflexive and deliberator components.

  9. The influence of TRP53 in the dose response of radiation-induced apoptosis, DNA repair and genomic stability in murine haematopoietic cells

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lemon, Jennifer A.; Taylor, Kristina; Verdecchia, Kyle; Phan, Nghi; Boreham, Douglas R.

    2014-01-01

    Apoptotic and DNA damage endpoints are frequently used as surrogate markers of cancer risk, and have been well-studied in the Trp53+/- mouse model. We report the effect of differing Trp53 gene status on the dose response of ionizing radiation exposures (0.01-2 Gy), with the unique perspective of determining if effects of gene status remain at extended time points. Here we report no difference in the dose response for radiation-induced DNA double-strand breaks in bone marrow and genomic instability (MN-RET levels) in peripheral blood, between wild-type (Trp53+/+) and heterozygous (Trp53+/-) mice. The dose response for Trp53+/+ mice showed higher initial levelsmore » of radiation-induced lymphocyte apoptosis relative to Trp53+/- between 0 and 1 Gy. Although this trend was observed up to 12 hours post-irradiation, both genotypes ultimately reached the same level of apoptosis at 14 hours, suggesting the importance of late-onset p53-independent apoptotic responses in this mouse model. Expected radiation-induced G1 cell cycle delay was observed in Trp53+/+ but not Trp53+/-. Although p53 has an important role in cancer risk, we have shown its influence on radiation dose response can be temporally variable. This research highlights the importance of caution when using haematopoietic endpoints as surrogates to extrapolate radiation-induced cancer risk estimation.« less

  10. Investigating the roles of MicroRNAs in biotic stress response induced by Rhizoctonia solani in rice

    SciTech Connect (OSTI)

    Syuhada, O. Nurfarahana; Kalaivani, N.

    2014-09-03

    Sheath blight disease, caused by Rhizoctonia solani 1802/KB was screened on two rice varieties, Oryza sativaindica cultivar MR219 and Oryza sativa indica cultivar UKMRC9. The disease symptom was severe in MR219 compared to UKMRC9. Total RNA from R. solani 1802/KB, infected rice leaves of MR219 and infected rice leaves of UKMRC9 were extracted using TRIzol reagent, purified and sent for small RNA sequencing. Three miRNA libraries were generated and analyzed. The libraries generated 65 805, 78 512 and 81 325 known miRNAs respectively. The structure of miRNA of these samples was predicted. The up-regulated and down-regulated of miRNAs target gene prediction and its target functions were discovered and were mainly related to the growth and development of metabolism, protein transport, transcriptional regulation, stress response, and hormone signaling and electron transfer. Sheath blight-induced differential expression of known miRNAs tends to targetMYB transcription factor, F-box proteins, NBS-LRR, leucine-rich repeat receptor protein kinases and zinc finger proteins. Detecting new miRNAs and measuring the expression profiles of known miRNAs is an important tasks required for a better understanding of various biological conditions. Therefore, further analysis using Gene Ontology Slim will be conducted to deduce some biological information from the datasets obtained.

  11. Structural response in FeCl2 (iron chloride) to pressure-induced electro-magnetic transitions

    SciTech Connect (OSTI)

    Taylor, R D; Rozenberg, G Kh; Pasternak, M P; Gorodetsky, P; Xu, W M; Dubrovinsky, L S; Le Bihan, T L

    2009-01-01

    High pressure (HP) synchrotron x-ray diffraction studies were carried out in FeCl{sub 2} together with resistivity (R) studies, at various temperatures and pressures to 65 GPa using diamond anvil cells. This work follows a previous HP {sup 57}Fe Mossbauer study in which two pressure-induced (PI) electronic transitions were found interpreted as: (i) quenching of the orbital-term contribution to the hyperfine field concurring with a tilting of the magnetic moment by 55 degrees and (ii) collapse of the magnetism concurring with a sharp decrease of the isomer shift (IS). The R(P,T) studies affirm that the cause the collapse of the magnetism is a PI p-d correlation breakdown, leading to an insulator-metal transition at {approx}45 GPa and is not due to a spi-Ir,crossover (S=2 {yields} S=0). The structure response to the pressure evolution of the two electronic phase transitions starting at low pressures (LP), through an intermediate phase (IP) 30-57 GPa, and culminating in a high-pressure phase (HP), P >32 GPa, can clearly be quantified. The IP-HP phases coexist through the 32-57 GPa range in which the HP abundance increases monotonically at the expense of the IP phase. At the LP-IP interface no volume change is detected, yet the c-axis increases and the a-axis shrinks by 0.21 Angstroms and 0.13 Angstroms, respectively. The fit of the equation of state of the combined LP-IP phases yields a bulk modulus K{sub 0} = 35.3(1.8) GPa. The intralayer CI-CI distances increases, but no change is observed in Fe-CI bond-length nor are there substantial changes in the interlayer spacing. The pressure-induced electronic IP-HP transition leads to a first-order structural phase transition characterized by a decrease in Fe-CI bond length and an abrupt drop in V(P) by {approx}3.5% accompanying the correlation breakdown. In this transition no symmetry change is detected,and the XRD data could be satisfactorily fitted with the CdI{sub 2} structure. The bulk modulus of the HP phase is practically the same as that of the LP-IP phases suggesting negligible changes in the phonon density of state.

  12. Synthesis and Application of an Environmentally Insensitive Cy3-Based Arsenical Fluorescent Probe to Identify Adaptive Microbial Responses Involving Proximal Dithiol Oxidation

    SciTech Connect (OSTI)

    Fu, Na; Su, Dian; Cort, John R.; Chen, Baowei; Xiong, Yijia; Qian, Weijun; Konopka, Allan; Bigelow, Diana J.; Squier, Thomas C.

    2013-03-06

    Reversible disulfide oxidation between proximal cysteines in proteins represents a common regulatory control mechanism to modulate flux through metabolic pathways in response to changing environmental conditions. To enable in vivo measurements of cellular redox changes linked to disulfide bond formation, we have synthesized a cell-permeable monosubstituted cyanine dye derivatized with arsenic (i.e., TRAP_Cy3) to trap and visualize dithiols in cytosolic proteins. Alkylation of reactive thiols prior to displacement of the bound TRAP-Cy3 by ethanedithiol permits facile protein capture and mass spectrometric identification of proximal reduced dithiols to the exclusion of individual cysteines. Applying TRAP_Cy3 to evaluate cellular responses to increases in oxygen and light levels in the photosynthetic microbe Synechococcus sp. PCC 7002, we observe large decreases in the abundance of reduced dithiols in cellular proteins, which suggest redox-dependent mechanisms involving the oxidation of proximal disulfides. Under these same growth conditions that result in the oxidation of proximal thiols, there is a reduction in the abundance of post-translational oxidative modifications involving nitrotyrosine and methionine sulfoxide formation. These results suggest that the redox status of proximal cysteines respond to environmental conditions, acting to regulate metabolic flux and minimize the formation of reactive oxygen species to decrease oxidative protein damage.

  13. The influence of TRP53 in the dose response of radiation-induced apoptosis, DNA repair and genomic stability in murine haematopoietic cells

    SciTech Connect (OSTI)

    Lemon, Jennifer A.; Taylor, Kristina; Verdecchia, Kyle; Phan, Nghi; Boreham, Douglas R.

    2014-01-01

    Apoptotic and DNA damage endpoints are frequently used as surrogate markers of cancer risk, and have been well-studied in the Trp53+/- mouse model. We report the effect of differing Trp53 gene status on the dose response of ionizing radiation exposures (0.01-2 Gy), with the unique perspective of determining if effects of gene status remain at extended time points. Here we report no difference in the dose response for radiation-induced DNA double-strand breaks in bone marrow and genomic instability (MN-RET levels) in peripheral blood, between wild-type (Trp53+/+) and heterozygous (Trp53+/-) mice. The dose response for Trp53+/+ mice showed higher initial levels of radiation-induced lymphocyte apoptosis relative to Trp53+/- between 0 and 1 Gy. Although this trend was observed up to 12 hours post-irradiation, both genotypes ultimately reached the same level of apoptosis at 14 hours, suggesting the importance of late-onset p53-independent apoptotic responses in this mouse model. Expected radiation-induced G1 cell cycle delay was observed in Trp53+/+ but not Trp53+/-. Although p53 has an important role in cancer risk, we have shown its influence on radiation dose response can be temporally variable. This research highlights the importance of caution when using haematopoietic endpoints as surrogates to extrapolate radiation-induced cancer risk estimation.

  14. Climate adaptation planning in practice: an evaluation of adaptation plans from three developed nations

    SciTech Connect (OSTI)

    Preston, Benjamin L; Westaway, Richard M.; Yuen, Emma J.

    2011-04-01

    Formal planning for climate change adaptation is emerging rapidly at a range of geo-political scales. This first generation of adaptation plans provides useful information regarding how institutions are framing the issue of adaptation and the range of processes that are recognized as being part of an adaptation response. To better understand adaptation planning among developed nations, a set of 57 adaptation plans from Australia, the United Kingdom and the United States was evaluated against a suite of 19 planning processes identified from existing guidance instruments for adaptation planning. Total scores among evaluated plans ranged from 16% of the maximum possible score to 61%, with an average of 37%. These results suggest adaptation plans are largely under-developed. Critical weaknesses in adaptation planning are related to limited consideration for non-climatic factors as well as neglect for issues of adaptive capacity including entitlements to various forms of capital needed for effective adaptation. Such gaps in planning suggest there are opportunities for institutions to make better use of existing guidance for adaptation planning and the need to consider the broader governance context in which adaptation will occur. In addition, the adaptation options prescribed by adaptation plans reflect a preferential bias toward low-risk capacity-building (72% of identified options) over the delivery of specific actions to reduce vulnerability. To the extent these findings are representative of the state of developed nation adaptation planning, there appear to be significant deficiencies in climate change preparedness, even among those nations often assumed to have the greatest adaptive capacity.

  15. Hydropower, adaptive management, and biodiversity

    SciTech Connect (OSTI)

    Wieringa, M.J.; Morton, A.G.

    1996-11-01

    Adaptive management is a policy framework within which an iterative process of decision making is allowed based on the observed responses to and effectiveness of previous decisions. The use of adaptive management allows science-based research and monitoring of natural resource and ecological community responses, in conjunction with societal values and goals, to guide decisions concerning man`s activities. The adaptive management process has been proposed for application to hydropower operations at Glen Canyon Dam on the Colorado River, a situation that requires complex balancing of natural resources requirements and competing human uses. This example is representative of the general increase in public interest in the operation of hydropower facilities and possible effects on downstream natural resources and of the growing conflicts between uses and users of river-based resources. This paper describes the adaptive management process, using the Glen Canyon Dam example, and discusses ways to make the process work effectively in managing downstream natural resources and biodiversity. 10 refs., 2 figs.

  16. Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis

    SciTech Connect (OSTI)

    Dong, Hui; Shi, Qiong; Song, Xiufang; Fu, Juanli; Hu, Lihua; Xu, Demei; Su, Chuanyang; Xia, Xiaomin; Song, Erqun; Song, Yang

    2015-07-01

    Our previous studies demonstrated that polychlorinated biphenyl (PCB) quinone induced oxidative DNA damage in HepG2 cells. To promote genomic integrity, DNA damage response (DDR) coordinates cell-cycle transitions, DNA repair and apoptosis. PCB quinone-induced cell cycle arrest and apoptosis have been documented, however, whether PCB quinone insult induce DNA repair signaling is still unknown. In this study, we identified the activation of DDR and corresponding signaling events in HepG2 cells upon the exposure to a synthetic PCB quinone, PCB29-pQ. Our data illustrated that PCB29-pQ induces the phosphorylation of p53, which was mediated by ataxia telangiectasia mutated (ATM) protein kinase. The observed phosphorylated histone H2AX (γ-H2AX) foci and the elevation of 8-hydroxy-2′-deoxyguanosine (8-OHdG) indicated that DDR was stimulated by PCB29-pQ treatment. Additionally, we found PCB29-pQ activates non-homologous end joining (NHEJ), base excision repair (BER) and nucleotide excision repair (NER) signalings. However, these repair pathways are not error-free processes and aberrant repair of DNA damage may cause the potential risk of carcinogenesis and mutagenesis. - Highlights: • Polychlorinated biphenyl quinone induces oxidative DNA damage in HepG2 cells. • The elevation of γ-H2AX and 8-OHdG indicates the activation of DNA damage response. • ATM-p53 signaling acts as the DNA damage sensor and effector. • Polychlorinated biphenyl quinone activates NHEJ, BER and NER signalings.

  17. Tat-CBR1 inhibits inflammatory responses through the suppressions of NF-κB and MAPK activation in macrophages and TPA-induced ear edema in mice

    SciTech Connect (OSTI)

    Kim, Young Nam; Kim, Dae Won; Jo, Hyo Sang; Shin, Min Jea; Ahn, Eun Hee; Ryu, Eun Ji; Yong, Ji In; Cha, Hyun Ju; Kim, Sang Jin; Yeo, Hyeon Ji; Youn, Jong Kyu; Hwang, Jae Hyeok; Jeong, Ji-Heon; Kim, Duk-Soo; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

    2015-07-15

    Human carbonyl reductase 1 (CBR1) plays a crucial role in cell survival and protects against oxidative stress response. However, its anti-inflammatory effects are not yet clearly understood. In this study, we examined whether CBR1 protects against inflammatory responses in macrophages and mice using a Tat-CBR1 protein which is able to penetrate into cells. The results revealed that purified Tat-CBR1 protein efficiently transduced into Raw 264.7 cells and inhibited lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2), nitric oxide (NO) and prostaglandin E{sub 2} (PGE{sub 2}) expression levels. In addition, Tat-CBR1 protein leads to decreased pro-inflammatory cytokine expression through suppression of nuclear transcription factor-kappaB (NF-κB) and mitogen activated protein kinase (MAPK) activation. Furthermore, Tat-CBR1 protein inhibited inflammatory responses in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation when applied topically. These findings indicate that Tat-CBR1 protein has anti-inflammatory properties in vitro and in vivo through inhibition of NF-κB and MAPK activation, suggesting that Tat-CBR1 protein may have potential as a therapeutic agent against inflammatory diseases. - Highlights: • Transduced Tat-CBR1 reduces LPS-induced inflammatory mediators and cytokines. • Tat-CBR1 inhibits MAPK and NF-κB activation. • Tat-CBR1 ameliorates inflammation response in vitro and in vivo. • Tat-CBR1 may be useful as potential therapeutic agent for inflammation.

  18. Aspirin-triggered resolvin D1 down-regulates inflammatory responses and protects against endotoxin-induced acute kidney injury

    SciTech Connect (OSTI)

    Chen, Jiao; Shetty, Sreerama; Zhang, Ping; Gao, Rong; Hu, Yuxin; Wang, Shuxia; Li, Zhenyu; Fu, Jian

    2014-06-01

    The presence of endotoxin in blood can lead to acute kidney injury (AKI) and septic shock. Resolvins, the endogenous lipid mediators derived from docosahexaenoic acid, have been reported to exhibit potent anti-inflammatory action. Using a mouse model of lipopolysaccharide (LPS)-induced AKI, we investigated the effects of aspirin-triggered resolvin D1 (AT-RvD1) on inflammatory kidney injury. Administration of AT-RvD1 1 h after LPS challenge protected the mice from kidney injury as indicated by the measurements of blood urea nitrogen, serum creatinine, and morphological alterations associated with tubular damage. The protective effects were evidenced by decreased neutrophil infiltration in the kidney indicating reduction in inflammation. AT-RvD1 treatment restored kidney cell junction protein claudin-4 expression, which was otherwise reduced after LPS challenge. AT-RvD1 treatment inhibited endotoxin-induced NF-κB activation and suppressed LPS-induced ICAM-1 and VCAM-1 expression in the kidney. Moreover, AT-RvD1 treatment markedly decreased LPS-induced IL-6 level in the kidney and blocked IL-6-mediated signaling including STAT3 and ERK phosphorylation. Our findings demonstrate that AT-RvD1 is a potent anti-inflammatory mediator in LPS-induced kidney injury, and AT-RvD1 has therapeutic potential against AKI during endotoxemia.

  19. Photo-induced change of dielectric response in BaCoSiO{sub 4} stuffed tridymite

    SciTech Connect (OSTI)

    Taniguchi, Hiroki Okamura, Takuma; Yamamoto, Takafumi; Okazaki, Ryuji; Terasaki, Ichiro; Moriwake, Hiroki; Kuwabara, Akihide; Itoh, Mitsuru

    2014-04-28

    The photodielectric effect is demonstrated in Mott-insulator BaCoSiO{sub 4} with a stuffed-tridymite-type structure under irradiation of visible light at 365?nm. The real part of dielectric permittivity is enhanced by ?300% with little increase of tan?? in a low-frequency region. Results of diffuse reflectance spectroscopy, first-principles calculations and dielectric measurements suggest that the photodielectric effect stems from a response of photo-excited electrons in an unoccupied upper-Hubbard band for 3d-orbitals of cobalt, which have significantly small mobility due to the unique configuration of Co ions in the stuffed-tridymite-type structure.

  20. TH-E-BRF-03: A Multivariate Interaction Model for Assessment of Hippocampal Vascular Dose-Response and Early Prediction of Radiation-Induced Neurocognitive Dysfunction

    SciTech Connect (OSTI)

    Farjam, R; Pramanik, P; Srinivasan, A; Chapman, C; Tsien, C; Lawrence, T; Cao, Y

    2014-06-15

    Purpose: Vascular injury could be a cause of hippocampal dysfunction leading to late neurocognitive decline in patients receiving brain radiotherapy (RT). Hence, our aim was to develop a multivariate interaction model for characterization of hippocampal vascular dose-response and early prediction of radiation-induced late neurocognitive impairments. Methods: 27 patients (17 males and 10 females, age 31–80 years) were enrolled in an IRB-approved prospective longitudinal study. All patients were diagnosed with a low-grade glioma or benign tumor and treated by 3-D conformal or intensity-modulated RT with a median dose of 54 Gy (50.4–59.4 Gy in 1.8− Gy fractions). Six DCE-MRI scans were performed from pre-RT to 18 months post-RT. DCE data were fitted to the modified Toft model to obtain the transfer constant of gadolinium influx from the intravascular space into the extravascular extracellular space, Ktrans, and the fraction of blood plasma volume, Vp. The hippocampus vascular property alterations after starting RT were characterized by changes in the hippocampal mean values of, μh(Ktrans)τ and μh(Vp)τ. The dose-response, Δμh(Ktrans/Vp)pre->τ, was modeled using a multivariate linear regression considering integrations of doses with age, sex, hippocampal laterality and presence of tumor/edema near a hippocampus. Finally, the early vascular dose-response in hippocampus was correlated with neurocognitive decline 6 and 18 months post-RT. Results: The μh(Ktrans) increased significantly from pre-RT to 1 month post-RT (p<0.0004). The multivariate model showed that the dose effect on Δμh(Ktrans)pre->1M post-RT was interacted with sex (p<0.0007) and age (p<0.00004), with the dose-response more pronounced in older females. Also, the vascular dose-response in the left hippocampus of females was significantly correlated with memory function decline at 6 (r = − 0.95, p<0.0006) and 18 (r = −0.88, p<0.02) months post-RT. Conclusion: The hippocampal vascular response to radiation could be sex and age dependent. The early hippocampal vascular dose-response could predict late neurocognitive dysfunction. (Support: NIH-RO1NS064973)

  1. Adaptive Sampling Proxy Application

    Energy Science and Technology Software Center (OSTI)

    2012-10-22

    ASPA is an implementation of an adaptive sampling algorithm [1-3], which is used to reduce the computational expense of computer simulations that couple disparate physical scales. The purpose of ASPA is to encapsulate the algorithms required for adaptive sampling independently from any specific application, so that alternative algorithms and programming models for exascale computers can be investigated more easily.

  2. Climate Change Adaptation Planning

    Broader source: Energy.gov [DOE]

    This course provides an introduction to planning for climate change impacts, with examples of tribes that have been going through the adaptation planning process. The course is intended for tribal...

  3. Leak test adapter for containers

    DOE Patents [OSTI]

    Hallett, Brian H.; Hartley, Michael S.

    1996-01-01

    An adapter is provided for facilitating the charging of containers and leak testing penetration areas. The adapter comprises an adapter body and stem which are secured to the container's penetration areas. The container is then pressurized with a tracer gas. Manipulating the adapter stem installs a penetration plug allowing the adapter to be removed and the penetration to be leak tested with a mass spectrometer. Additionally, a method is provided for using the adapter.

  4. Sensor response rate accelerator

    DOE Patents [OSTI]

    Vogt, Michael C.

    2002-01-01

    An apparatus and method for sensor signal prediction and for improving sensor signal response time, is disclosed. An adaptive filter or an artificial neural network is utilized to provide predictive sensor signal output and is further used to reduce sensor response time delay.

  5. Adapting to Survive

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Adapting to Survive Lesson on how climate and environment affect people in Alaska. Grade: 6 th - 9th Duration: Flexible. Up to the teacher. This lesson could take up to a week. 30 minutes to 1hr a day. Goal: This lesson is an introduction to a study of Native Alaskans of the past. In order to survive, humans have always had the same basic physical needs. The physical characteristics of the land determine in large part how we satisfy those needs, and as the earth changes we must also adapt to

  6. Telescope Adaptive Optics Code

    Energy Science and Technology Software Center (OSTI)

    2005-07-28

    The Telescope AO Code has general adaptive optics capabilities plus specialized models for three telescopes with either adaptive optics or active optics systems. It has the capability to generate either single-layer or distributed Kolmogorov turbulence phase screens using the FFT. Missing low order spatial frequencies are added using the Karhunen-Loeve expansion. The phase structure curve is extremely dose to the theoreUcal. Secondly, it has the capability to simulate an adaptive optics control systems. The defaultmore » parameters are those of the Keck II adaptive optics system. Thirdly, it has a general wave optics capability to model the science camera halo due to scintillation from atmospheric turbulence and the telescope optics. Although this capability was implemented for the Gemini telescopes, the only default parameter specific to the Gemini telescopes is the primary mirror diameter. Finally, it has a model for the LSST active optics alignment strategy. This last model is highly specific to the LSST« less

  7. Online adaptation and verification of VMAT

    SciTech Connect (OSTI)

    Crijns, Wouter; Defraene, Gilles; Depuydt, Tom; Haustermans, Karin; Van Herck, Hans; Maes, Frederik; Van den Heuvel, Frank

    2015-07-15

    Purpose: This work presents a method for fast volumetric modulated arc therapy (VMAT) adaptation in response to interfraction anatomical variations. Additionally, plan parameters extracted from the adapted plans are used to verify the quality of these plans. The methods were tested as a prostate class solution and compared to replanning and to their current clinical practice. Methods: The proposed VMAT adaptation is an extension of their previous intensity modulated radiotherapy (IMRT) adaptation. It follows a direct (forward) planning approach: the multileaf collimator (MLC) apertures are corrected in the beam’s eye view (BEV) and the monitor units (MUs) are corrected using point dose calculations. All MLC and MU corrections are driven by the positions of four fiducial points only, without need for a full contour set. Quality assurance (QA) of the adapted plans is performed using plan parameters that can be calculated online and that have a relation to the delivered dose or the plan quality. Five potential parameters are studied for this purpose: the number of MU, the equivalent field size (EqFS), the modulation complexity score (MCS), and the components of the MCS: the aperture area variability (AAV) and the leaf sequence variability (LSV). The full adaptation and its separate steps were evaluated in simulation experiments involving a prostate phantom subjected to various interfraction transformations. The efficacy of the current VMAT adaptation was scored by target mean dose (CTV{sub mean}), conformity (CI{sub 95%}), tumor control probability (TCP), and normal tissue complication probability (NTCP). The impact of the adaptation on the plan parameters (QA) was assessed by comparison with prediction intervals (PI) derived from a statistical model of the typical variation of these parameters in a population of VMAT prostate plans (n = 63). These prediction intervals are the adaptation equivalent of the tolerance tables for couch shifts in the current clinical practice. Results: The proposed adaptation of a two-arc VMAT plan resulted in the intended CTV{sub mean} (Δ ≤ 3%) and TCP (ΔTCP ≤ 0.001). Moreover, the method assures the intended CI{sub 95%} (Δ ≤ 11%) resulting in lowered rectal NTCP for all cases. Compared to replanning, their adaptation is faster (13 s vs 10 min) and more intuitive. Compared to the current clinical practice, it has a better protection of the healthy tissue. Compared to IMRT, VMAT is more robust to anatomical variations, but it is also less sensitive to the different correction steps. The observed variations of the plan parameters in their database included a linear dependence on the date of treatment planning and on the target radius. The MCS is not retained as QA metric due to a contrasting behavior of its components (LSV and AAV). If three out of four plan parameters (MU, EqFS, AAV, and LSV) need to lie inside a 50% prediction interval (3/4—50%PI), all adapted plans will be accepted. In contrast, all replanned plans do not meet this loose criterion, mainly because they have no connection to the initially optimized and verified plan. Conclusions: A direct (forward) VMAT adaptation performs equally well as (inverse) replanning but is faster and can be extended to real-time adaptation. The prediction intervals for the machine parameters are equivalent to the tolerance tables for couch shifts in the current clinical practice. A 3/4—50%PI QA criterion accepts all the adapted plans but rejects all the replanned plans.

  8. Adaptive control for accelerators

    DOE Patents [OSTI]

    Eaton, Lawrie E.; Jachim, Stephen P.; Natter, Eckard F.

    1991-01-01

    An adaptive feedforward control loop is provided to stabilize accelerator beam loading of the radio frequency field in an accelerator cavity during successive pulses of the beam into the cavity. A digital signal processor enables an adaptive algorithm to generate a feedforward error correcting signal functionally determined by the feedback error obtained by a beam pulse loading the cavity after the previous correcting signal was applied to the cavity. Each cavity feedforward correcting signal is successively stored in the digital processor and modified by the feedback error resulting from its application to generate the next feedforward error correcting signal. A feedforward error correcting signal is generated by the digital processor in advance of the beam pulse to enable a composite correcting signal and the beam pulse to arrive concurrently at the cavity.

  9. CLAMR (Compute Language Adaptive Mesh Refinement)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CLAMR (Compute Language Adaptive Mesh Refinement) CLAMR (Compute Language Adaptive Mesh Refinement) CLAMR (Compute Language Adaptive Mesh Refinement) is being developed as a DOE...

  10. Climate Change Adaptation | Department of Energy

    Energy Savers [EERE]

    Climate Change Adaptation Climate Change Adaptation DOE is adapting to climate change by applying a risk-based resiliency approach to identify and minimize climate-related...

  11. AfricaAdapt | Open Energy Information

    Open Energy Info (EERE)

    Africa Adapt AgencyCompany Organization AfricaAdapt Resource Type Training materials, Lessons learnedbest practices Website http:www.africa-adapt.netAA UN Region Eastern...

  12. Climate Adaptation for Transportation | Open Energy Information

    Open Energy Info (EERE)

    Climate Adaptation for Transportation (Redirected from 03 Climate Adaptation for Transportation) Jump to: navigation, search Tool Summary LAUNCH TOOL Name: 03 Climate Adaptation...

  13. Adaptive Energy Grid

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Control of a Flexible, Adaptive Energy Grid !"#$%"&%'&"&()*+%,-./-"(&*"0.-"+.-1&.,2-"+2$&01&!"#$%"&3.-,.-"+%.#4&"&5.67822$& 9"-+%#&3.(,"#14&:.-&+82&;#%+2$&!+"+2'&<2,"-+(2#+&.:&=#2-/1>'&?"+%.#"*&?)6*2"-& !26)-%+1&@$(%#%'+-"+%.#&)#$2-&6.#+-"6+&&

  14. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  15. Sulforaphane prevents pulmonary damage in response to inhaled arsenic by activating the Nrf2-defense response

    SciTech Connect (OSTI)

    Zheng, Yi; Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona, 1703 East Mabel Street, Tucson, AZ 85721 ; Tao, Shasha; Lian, Fangru; Chau, Binh T.; Chen, Jie; Sun, Guifan; Fang, Deyu; Lantz, R. Clark; Arizona Cancer Center, University of Arizona, 1515 North Campbell Avenue, Tucson, AZ 85724 ; Zhang, Donna D.

    2012-12-15

    Exposure to arsenic is associated with an increased risk of lung disease. Novel strategies are needed to reduce the adverse health effects associated with arsenic exposure in the lung. Nrf2, a transcription factor that mediates an adaptive cellular defense response, is effective in detoxifying environmental insults and prevents a broad spectrum of diseases induced by environmental exposure to harmful substances. In this report, we tested whether Nrf2 activation protects mice from arsenic-induced toxicity. We used an in vivo arsenic inhalation model that is highly relevant to low environmental human exposure to arsenic-containing dusts. Two-week exposure to arsenic-containing dust resulted in pathological alterations, oxidative DNA damage, and mild apoptotic cell death in the lung; all of which were blocked by sulforaphane (SF) in an Nrf2-dependent manner. Mechanistically, SF-mediated activation of Nrf2 alleviated inflammatory responses by modulating cytokine production. This study provides strong evidence that dietary intervention targeting Nrf2 activation is a feasible approach to reduce adverse health effects associated with arsenic exposure. -- Highlights: ? Exposed to arsenic particles and/or SF have elevated Nrf2 and its target genes. ? Sulforaphane prevents pathological alterations, oxidative damage and cell death. ? Sulforaphane alleviates infiltration of inflammatory cells into the lungs. ? Sulforaphane suppresses arsenic-induced proinflammatory cytokine production.

  16. Sulforaphane, a cancer chemopreventive agent, induces pathways associated with membrane biosynthesis in response to tissue damage by aflatoxin B{sub 1}

    SciTech Connect (OSTI)

    Techapiesancharoenkij, Nirachara; Fiala, Jeannette L.A.; Navasumrit, Panida; Croy, Robert G.; Wogan, Gerald N.; Groopman, John D.; Ruchirawat, Mathuros; Essigmann, John M.

    2015-01-01

    Aflatoxin B{sub 1} (AFB{sub 1}) is one of the major risk factors for liver cancer globally. A recent study showed that sulforaphane (SF), a potent inducer of phase II enzymes that occurs naturally in widely consumed vegetables, effectively induces hepatic glutathione S-transferases (GSTs) and reduces levels of hepatic AFB{sub 1}-DNA adducts in AFB{sub 1}-exposed Sprague Dawley rats. The present study characterized the effects of SF pre-treatment on global gene expression in the livers of similarly treated male rats. Combined treatment with AFB{sub 1} and SF caused reprogramming of a network of genes involved in signal transduction and transcription. Changes in gene regulation were observable 4 h after AFB{sub 1} administration in SF-pretreated animals and may reflect regeneration of cells in the wake of AFB{sub 1}-induced hepatotoxicity. At 24 h after AFB{sub 1} administration, significant induction of genes that play roles in cellular lipid metabolism and acetyl-CoA biosynthesis was detected in SF-pretreated AFB{sub 1}-dosed rats. Induction of this group of genes may indicate a metabolic shift toward glycolysis and fatty acid synthesis to generate and maintain pools of intermediate molecules required for tissue repair, cell growth and compensatory hepatic cell proliferation. Collectively, gene expression data from this study provide insights into molecular mechanisms underlying the protective effects of SF against AFB{sub 1} hepatotoxicity and hepatocarcinogenicity, in addition to the chemopreventive activity of this compound as a GST inducer. - Highlights: • This study revealed sulforaphane (SF)-deregulated gene sets in aflatoxin B{sub 1} (AFB{sub 1})-treated rat livers. • SF redirects biochemical networks toward lipid biosynthesis in AFB{sub 1}-dosed rats. • SF enhanced gene sets that would be expected to favor cell repair and regeneration.

  17. Adaptive multiconfigurational wave functions

    SciTech Connect (OSTI)

    Evangelista, Francesco A.

    2014-03-28

    A method is suggested to build simple multiconfigurational wave functions specified uniquely by an energy cutoff ?. These are constructed from a model space containing determinants with energy relative to that of the most stable determinant no greater than ?. The resulting ?-CI wave function is adaptive, being able to represent both single-reference and multireference electronic states. We also consider a more compact wave function parameterization (?+SD-CI), which is based on a small ?-CI reference and adds a selection of all the singly and doubly excited determinants generated from it. We report two heuristic algorithms to build ?-CI wave functions. The first is based on an approximate prescreening of the full configuration interaction space, while the second performs a breadth-first search coupled with pruning. The ?-CI and ?+SD-CI approaches are used to compute the dissociation curve of N{sub 2} and the potential energy curves for the first three singlet states of C{sub 2}. Special attention is paid to the issue of energy discontinuities caused by changes in the size of the ?-CI wave function along the potential energy curve. This problem is shown to be solvable by smoothing the matrix elements of the Hamiltonian. Our last example, involving the Cu{sub 2}O{sub 2}{sup 2+} core, illustrates an alternative use of the ?-CI method: as a tool to both estimate the multireference character of a wave function and to create a compact model space to be used in subsequent high-level multireference coupled cluster computations.

  18. Carbon dioxide effects research and assessment program. Environmental and societal consequences of a possible CO/sub 2/-induced climate change: volume II, part I. Response of the West Antarctic ice sheet to CO/sub 2/-induced climatic warming

    SciTech Connect (OSTI)

    Bentley, C.

    1982-04-01

    The paper proposes a research plan to deal with the question of what the response of the West Antarctic Ice Sheet would be to a rise in global temperatures caused by an anthropogenic CO/sub 2/ buildup in the atmosphere. The plan is designed to answer the following questions: (1) how fast is the ice mass changing now, and why; (2) how will the boundary conditions that affect the ice sheet respond to an atmospheric temperature change and how are those boundary conditions changing now; (3) what will be the response of the ice sheet to changes in boundary conditions; and (4) what can be learned by analogy with what has happened in the past. (ACR)

  19. 2014 DOE Climate Change Adaptation Plan

    Energy Savers [EERE]

    4 U.S. Department of Energy June 2014 DOE Climate Change Adaptation Plan Table of Contents Climate Change Adaptation Plan ................................................................................................................................2 Impetus for Action ..................................................................................................................................................2 The DOE Mission and Climate Change Adaptation

  20. Parametric Adaptive Model Based Diagnostics

    Broader source: Energy.gov [DOE]

    A model-based adaptive, robust technology is presented for on-board diagnostics of failure of diesel engine emission control devices and ethanol estimation of flex-fuel vehicles.

  1. Mainstreaming Climate Change Adaptation into Development Planning...

    Open Energy Info (EERE)

    Climate Change Adaptation into Development Planning: A Guide for Practitioners Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Mainstreaming Climate Change Adaptation...

  2. Adaptive Materials Inc | Open Energy Information

    Open Energy Info (EERE)

    Michigan Zip: MI 48108 Product: Adaptive Materials Inc (AMI) is a developer of portable fuel cell technology. References: Adaptive Materials Inc1 This article is a stub. You...

  3. WeADAPT | Open Energy Information

    Open Energy Info (EERE)

    weADAPT Sector Energy, Land, Water, Climate Resource Type Training materials, Lessons learnedbest practices Website http:www.weadapt.org References weADAPT1...

  4. Climate Adaptation for Transportation | Open Energy Information

    Open Energy Info (EERE)

    Climate Adaptation for Transportation Jump to: navigation, search Tool Summary LAUNCH TOOL Name: 03 Climate Adaptation for Transportation AgencyCompany Organization: AASHTO...

  5. Adaptive Liquid Crystal Windows

    SciTech Connect (OSTI)

    Taheri, Bahman; Bodnar, Volodymyr

    2011-12-31

    Energy consumption by private and commercial sectors in the U.S. has steadily grown over the last decade. The uncertainty in future availability of imported oil, on which the energy consumption relies strongly, resulted in a dramatic increase in the cost of energy. About 20% of this consumption are used to heat and cool houses and commercial buildings. To reduce dependence on the foreign oil and cut down emission of greenhouse gases, it is necessary to eliminate losses and reduce total energy consumption by buildings. To achieve this goal it is necessary to redefine the role of the conventional windows. At a minimum, windows should stop being a source for energy loss. Ideally, windows should become a source of energy, providing net gain to reduce energy used to heat and cool homes. It is possible to have a net energy gain from a window if its light transmission can be dynamically altered, ideally electronically without the need of operator assistance, providing optimal control of the solar gain that varies with season and climate in the U.S. In addition, the window must not require power from the building for operation. Resolution of this problem is a societal challenge and of national interest and will have a broad global impact. For this purpose, the year-round, allclimate window solution to provide an electronically variable solar heat gain coefficient (SHGC) with a wide dynamic range is needed. AlphaMicron, Inc. (AMI) developed and manufactured 1ft 1ft prototype panels for the worlds first auto-adjusting Adaptive Liquid Crystal Windows (ALCWs) that can operate from sunlight without the need for external power source and demonstrate an electronically adjustable SHGC. This novel windows are based on AlphaMicrons patented e-Tint technology, a guesthost liquid crystal system implemented on flexible, optically clear plastic films. This technology is suitable both for OEM and aftermarket (retro-fitting) lamination to new and existing windows. Low level of power consumption by ALCWs allows for on-board power electronics for automatic matching of transmission through windows to varying climate conditions without drawing the power from the power grid. ALCWs are capable of transmitting more sunlight in winters to assist in heating and less sunlight in summers to minimize overheating. As such, they can change the window from being a source of energy loss to a source of energy gain. In addition, the scalable AMIs roll-to-roll process, proved by making 1ft 1ftALCW prototype panels, allows for cost-effective production of large-scale window panels along with capability to change easily their color and shape. In addition to architectural glazing in houses and commercial buildings, ALCWs can be used in other applications where control of sunlight is needed, such as green houses, used by commercial produce growers and botanical gardens, cars, aircrafts, etc.

  6. Automated Grid Disruption Response System: Robust Adaptive Topology Control (RATC)

    SciTech Connect (OSTI)

    2012-03-01

    GENI Project: The RATC research team is using topology control as a mechanism to improve system operations and manage disruptions within the electric grid. The grid is subject to interruption from cascading faults caused by extreme operating conditions, malicious external attacks, and intermittent electricity generation from renewable energy sources. The RATC system is capable of detecting, classifying, and responding to grid disturbances by reconfiguring the grid in order to maintain economically efficient operations while guaranteeing reliability. The RATC system would help prevent future power outages, which account for roughly $80 billion in losses for businesses and consumers each year. Minimizing the time it takes for the grid to respond to expensive interruptions will also make it easier to integrate intermittent renewable energy sources into the grid.

  7. Training Adaptive Decision-Making.

    SciTech Connect (OSTI)

    Abbott, Robert G.; Forsythe, James C.

    2014-10-01

    Adaptive Thinking has been defined here as the capacity to recognize when a course of action that may have previously been effective is no longer effective and there is need to adjust strategy. Research was undertaken with human test subjects to identify the factors that contribute to adaptive thinking. It was discovered that those most effective in settings that call for adaptive thinking tend to possess a superior capacity to quickly and effectively generate possible courses of action, as measured using the Category Generation test. Software developed for this research has been applied to develop capabilities enabling analysts to identify crucial factors that are predictive of outcomes in fore-on-force simulation exercises.

  8. Epigenomic Adaptation to Low Dose Radiation

    SciTech Connect (OSTI)

    Gould, Michael N.

    2015-06-30

    The overall hypothesis of this grant application is that the adaptive responses elicited by low dose ionizing radiation (LDIR) result in part from heritable DNA methylation changes in the epigenome. In the final budget period at the University of Wisconsin-Madison, we will specifically address this hypothesis by determining if the epigenetically labile, differentially methylated regions (DMRs) that regulate parental-specific expression of imprinted genes are deregulated in agouti mice by low dose radiation exposure during gestation. This information is particularly important to ascertain given the 1) increased human exposure to medical sources of radiation; 2) increased number of people predicted to live and work in space; and 3) enhanced citizen concern about radiation exposure from nuclear power plant accidents and terrorist ‘dirty bombs.’

  9. Adaptive protection algorithm and system

    DOE Patents [OSTI]

    Hedrick, Paul (Pittsburgh, PA) [Pittsburgh, PA; Toms, Helen L. (Irwin, PA) [Irwin, PA; Miller, Roger M. (Mars, PA) [Mars, PA

    2009-04-28

    An adaptive protection algorithm and system for protecting electrical distribution systems traces the flow of power through a distribution system, assigns a value (or rank) to each circuit breaker in the system and then determines the appropriate trip set points based on the assigned rank.

  10. Adaptive wiener image restoration kernel

    DOE Patents [OSTI]

    Yuan, Ding

    2007-06-05

    A method and device for restoration of electro-optical image data using an adaptive Wiener filter begins with constructing imaging system Optical Transfer Function, and the Fourier Transformations of the noise and the image. A spatial representation of the imaged object is restored by spatial convolution of the image using a Wiener restoration kernel.

  11. Mutations in global regulators lead to metabolic selection during adaptation to complex environments

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Saxer, Gerda; Krepps, Michael D.; Merkley, Eric D.; Ansong, Charles; Deatherage Kaiser, Brooke L.; Valovska, Marie -Thérèse; Ristic, Nikola; Yeh, Ping T.; Prakash, Vittal P.; Leiser, Owen P.; et al

    2014-12-11

    Adaptation to ecologically complex environments can provide insights into the evolutionary dynamics and functional constraints encountered by organisms during natural selection. Unlike adaptation to a single limiting resource, adaptation to a new environment with abundant and varied resources can be difficult to achieve by small incremental changes since many mutations are required to achieve even modest gains in fitness. Since changing complex environments are quite common in nature, we investigated how such an epistatic bottleneck can be avoided to allow rapid adaptation. We show that adaptive mutations arise repeatedly in independently evolved populations in the context of greatly increased geneticmore » and phenotypic diversity. We go on to show that weak selection requiring substantial metabolic reprogramming can be readily achieved by mutations in the global response regulator arcA and the stress response regulator rpoS. We identified 46 unique single-nucleotide variants of arcA and 18 mutations in rpoS, nine of which resulted in stop codons or large deletions, suggesting that a subtle modulation of ArcA function and knockouts of rpoS are largely responsible for the metabolic shifts leading to adaptation. These mutations allow a higher order “metabolic selection” that eliminates epistatic bottlenecks, which could occur when many changes would be required. Proteomic and carbohydrate analysis of adapting E. coli populations revealed an up-regulation of enzymes associated with the TCA cycle and amino acid metabolism and an increase in the secretion of putrescine. The overall effect of adaptation across populations is to redirect and efficiently utilize uptake and catabolism of abundant amino acids. Concomitantly, there is a pronounced spread of more ecologically limited strains that results from specialization through metabolic erosion. Remarkably, the global regulators arcA and rpoS can provide a “one-step” mechanism of adaptation to a novel environment, which highlights the importance of global resource management as a powerful strategy to adaptation.« less

  12. African Adaptation Programme | Open Energy Information

    Open Energy Info (EERE)

    AgencyCompany Organization United Nations Development Programme, Government of Japan Topics Adaptation, Finance, Implementation, Policiesdeployment programs Website...

  13. Mechanisms of radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.

    1996-10-01

    In the process of identifying genes differentially expressed in cells exposed ultraviolet radiation, we have identified a transcript having a 26-bp region that is highly conserved in a variety of species including Bacillus circulans, yeast, pumpkin, Drosophila, mouse, and man. When the 5` region (flanking region or UTR) of a gene, the sequence is predominantly in +/+ orientation with respect to the coding DNA strand; while in the coding region and the 3` region (UTR), the sequence is most frequently in the +/-orientation with respect to the coding DNA strand. In two genes, the element is split into two parts; however, in most cases, it is found only once but with a minimum of 11 consecutive nucleotides precisely depicting the original sequence. The element is found in a large number of different genes with diverse functions (from human ras p21 to B. circulans chitonase). Gel shift assays demonstrated the presence of a protein in HeLa cell extracts that binds to the sense and antisense single-stranded consensus oligomers, as well as to the double- stranded oligonucleotide. When double-stranded oligomer was used, the size shift demonstrated as additional protein-oligomer complex larger than the one bound to either sense or antisense single-stranded consensus oligomers alone. It is speculated either that this element binds to protein(s) important in maintaining DNA is a single-stranded orientation for transcription or, alternatively that this element is important in the transcription-coupled DNA repair process.

  14. Optical Design for Extremely Large Telescope Adaptive Optics...

    Office of Scientific and Technical Information (OSTI)

    Large Telescope Adaptive Optics Systems Citation Details In-Document Search Title: Optical Design for Extremely Large Telescope Adaptive Optics Systems Designing an adaptive ...

  15. Regional Climate Change Adaptation Platform for Asia | Open Energy...

    Open Energy Info (EERE)

    Climate Change Adaptation Platform for Asia Jump to: navigation, search Logo: Regional Climate Change Adaptation Platform for Asia Name Regional Climate Change Adaptation Platform...

  16. 2014 DOE Climate Change Adaptation Plan | Department of Energy

    Office of Environmental Management (EM)

    Climate Change Adaptation Plan 2014 DOE Climate Change Adaptation Plan Document presents the U.S. Department of Energy (DOE) 2014 plan for adapting to climate change....

  17. Adaptive capture of expert behavior

    SciTech Connect (OSTI)

    Jones, R.D.; Barrett, C.L.; Hand, U.; Gordon, R.C.

    1994-08-01

    The authors smoothed and captured a set of expert rules with adaptive networks. The motivation for doing this is discussed. (1) Smoothing leads to stabler control actions. (2) For some sets of rules, the evaluation of the rules can be sped up. This is important in large-scale simulations where many intelligent elements are present. (3) Variability of the intelligent elements can be achieved by adjusting the weights in an adaptive network. (4) After capture has occurred, the weights can be adjusted based on performance criteria. The authors thus have the capability of learning a new set of rules that lead to better performance. The set of rules the authors chose to capture were based on a set of threat determining rules for tank commanders. The approach in this paper: (1) They smoothed the rules. The rule set was converted into a simple set of arithmetic statements. Continuous, non-binary inputs, are now permitted. (2) An operational measure of capturability was developed. (3) They chose four candidate networks for the rule set capture: (a) multi-linear network, (b) adaptive partial least squares, (c) connectionist normalized local spline (CNLS) network, and (d) CNLS net with a PLS preprocessor. These networks were able to capture the rule set to within a few percent. For the simple tank rule set, the multi-linear network performed the best. When the rules were modified to include more nonlinear behavior, CNLS net performed better than the other three nets which made linear assumptions. (4) The networks were tested for robustness to input noise. Noise levels of plus or minus 10% had no real effect on the network performance. Noise levels in the plus or minus 30% range degraded performance by a factor of two. Some performance enhancement occurred when the networks were trained with noisy data. (5) The scaling of the evaluation time was calculated. (6) Human variation can be mimicked in all the networks by perturbing the weights.

  18. Demand Response for Ancillary Services

    Broader source: Energy.gov [DOE]

    Methodologies used to study grid integration of variable generation can be adapted to the study of demand response. In the present work, we describe and implement a methodology to construct detailed temporal and spatial representations of demand response resources and to incorporate those resources into power system models. In addition, the paper outlines ways to evaluate barriers to implementation. We demonstrate how the combination of these three analyses can be used to assess economic value of the realizable potential of demand response for ancillary services.

  19. Demand Response for Ancillary Services

    SciTech Connect (OSTI)

    Alkadi, Nasr E; Starke, Michael R

    2013-01-01

    Many demand response resources are technically capable of providing ancillary services. In some cases, they can provide superior response to generators, as the curtailment of load is typically much faster than ramping thermal and hydropower plants. Analysis and quantification of demand response resources providing ancillary services is necessary to understand the resources economic value and impact on the power system. Methodologies used to study grid integration of variable generation can be adapted to the study of demand response. In the present work, we describe and illustrate a methodology to construct detailed temporal and spatial representations of the demand response resource and to examine how to incorporate those resources into power system models. In addition, the paper outlines ways to evaluate barriers to implementation. We demonstrate how the combination of these three analyses can be used to translate the technical potential for demand response providing ancillary services into a realizable potential.

  20. NEPA, monitoring, and adaptive management

    SciTech Connect (OSTI)

    Carpenter, R.A.

    1995-12-01

    Getting concerns about the environment on the decision making table before Federal actions are taken is the recognized business of the National Environmental Policy Act (NEPA), but keeping them there is just as important. Human interventions into natural systems seldom proceed as originally planned. Scientific uncertainties prevent environmental impacts from being reliably or precisely predicted. Thus, the style of management must provide for monitoring to guide mid-course corrections adapting to inevitable surprises. the one time, pre-approval EA/EIS procedure remains essential but is not sufficient to assure the goal of NEPA {open_quotes}to...maintain conditions under which man and nature can exist in productive harmony...{close_quotes} (NEPA, 1969). This paper explores the extent to which NEPA encourages continuous assessment for timely feedback to managers, and the practical difficulties involved in doing so.

  1. Electricity Market Complex Adaptive System

    Energy Science and Technology Software Center (OSTI)

    2004-10-14

    EMCAS is a model developed for the simulation and analysis of electricity markets. As power markets are relatively new and still continue to evolve, there is a growing need for advanced modeling approaches that simulate the behavior of electricity markets over time and how market participants may act and react to the changing economic, financial, and regulatory environments in which they operate. A new and rather promising approach applied in the EMCAS software is tomore » model the electricity market as a complex adaptive system using an agent-based modeling and simulation scheme. With its unique combination of various novel approaches, the Agent Based Modeling System (ABMS) provides the ability to capture and investigate the complex interactions between the physical infrastructures (generation, transmission, and distribution) and the economic behavior of market participants that are a trademark of the newly emerging markets.« less

  2. Adaptive Intrusion Data System (AIDS)

    SciTech Connect (OSTI)

    Corlis, N. E.

    1980-05-01

    The adaptive intrusion data system (AIDS) was developed to collect data from intrusion alarm sensors as part of an evaluation system to improve sensor performance. AIDS is a unique data system which uses computer controlled data systems, video cameras and recorders, analog-to-digital conversion, environmental sensors, and digital recorders to collect sensor data. The data can be viewed either manually or with a special computerized data-reduction system which adds new data to a data base stored on a magnetic disc recorder. This report provides a synoptic account of the AIDS as it presently exists. Modifications to the purchased subsystems are described, and references are made to publications which describe the Sandia-designed subsystems.

  3. Downhole tool adapted for telemetry

    DOE Patents [OSTI]

    Hall, David R.; Fox, Joe

    2010-12-14

    A cycleable downhole tool such as a Jar, a hydraulic hammer, and a shock absorber adapted for telemetry. This invention applies to other tools where the active components of the tool are displaced when the tool is rotationally or translationally cycled. The invention consists of inductive or contact transmission rings that are connected by an extensible conductor. The extensible conductor permits the transmission of the signal before, after, and during the cycling of the tool. The signal may be continuous or intermittent during cycling. The invention also applies to downhole tools that do not cycle, but in operation are under such stress that an extensible conductor is beneficial. The extensible conductor may also consist of an extensible portion and a fixed portion. The extensible conductor also features clamps that maintain the conductor under stresses greater than that seen by the tool, and seals that are capable of protecting against downhole pressure and contamination.

  4. Cubit Adaptive Meshing Algorithm Library

    Energy Science and Technology Software Center (OSTI)

    2004-09-01

    CAMAL (Cubit adaptive meshing algorithm library) is a software component library for mesh generation. CAMAL 2.0 includes components for triangle, quad and tetrahedral meshing. A simple Application Programmers Interface (API) takes a discrete boundary definition and CAMAL computes a quality interior unstructured grid. The triangle and quad algorithms may also import a geometric definition of a surface on which to define the grid. CAMAL’s triangle meshing uses a 3D space advancing front method, the quadmore » meshing algorithm is based upon Sandia’s patented paving algorithm and the tetrahedral meshing algorithm employs the GHS3D-Tetmesh component developed by INRIA, France.« less

  5. Climate Change Adaptation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Climate Change Adaptation Climate Change Adaptation Mission The Climate Change Adaption team affirms the overall DOE commitment to plan for and manage the short- and long-term effects of climate change, as deemed appropriate for LM operations and approved by LM, as defined in: Executive Order (EO) 13693, Planning for Federal Sustainability in the Next Decade, and EO 13653, Preparing the United States for the Impacts of Climate Change The team endorses the President's Climate Action Plan,

  6. Results of adaptive feedforward on GTA

    SciTech Connect (OSTI)

    Ziomek, C.D.; Denney, P.M.; Regan, A.H.; Lynch, M.T.; Jachim, S.P.; Eaton, L.E.; Natter, E.F.

    1993-06-01

    This paper presents the results of the adaptive feedforward system in use on the Ground Test Accelerator (GTA). The adaptive feedforward system was shown to correct repetitive, high-frequency errors in the amplitude and phase of the RF field of the pulsed accelerator. The adaptive feedforward system was designed as an augmentation to the RF field feedback control system and was able to extend the closed-loop bandwidth and disturbance rejection by a factor of ten. Within a second implementation, the adaptive feedforward hardware was implemented in place of the feedback control system and was shown to negate both beam transients and phase droop in the klystron amplifier.

  7. Results of adaptive feedforward on GTA

    SciTech Connect (OSTI)

    Ziomek, C.D.; Denney, P.M.; Regan, A.H.; Lynch, M.T.; Jachim, S.P.; Eaton, L.E.; Natter, E.F.

    1993-01-01

    This paper presents the results of the adaptive feedforward system in use on the Ground Test Accelerator (GTA). The adaptive feedforward system was shown to correct repetitive, high-frequency errors in the amplitude and phase of the RF field of the pulsed accelerator. The adaptive feedforward system was designed as an augmentation to the RF field feedback control system and was able to extend the closed-loop bandwidth and disturbance rejection by a factor of ten. Within a second implementation, the adaptive feedforward hardware was implemented in place of the feedback control system and was shown to negate both beam transients and phase droop in the klystron amplifier.

  8. Climate Change Adaptation Technical Fact Sheet: Contaminated...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of potential climate change vulnerabilities and (2) presenting possible adaptation measures that may be considered to increase a remedy's resilience to climate change impacts. ...

  9. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Structure Suggests Role as Molecular Adapter Print To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known as the...

  10. Assessing Climate Change Impacts, Vulnerability and Adaptation...

    Open Energy Info (EERE)

    Climate Change Impacts, Vulnerability and Adaptation: The Case of Pantabangan-Carranglan Watershed Jump to: navigation, search Name Assessing Climate Change Impacts, Vulnerability...

  11. Training for Climate Adaptation in Conservation

    Broader source: Energy.gov [DOE]

    The Wildlife Conservation Society and the Northern Institute of Applied Climate Science are hosting this two-day training for climate adaptation.

  12. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure Suggests Role as Molecular Adapter Print To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known as the...

  13. Adaptive management: a paradigm for remediation of public facilities

    SciTech Connect (OSTI)

    Janecky, David R; Whicker, Jeffrey J; Doerr, Ted B

    2009-01-01

    Public facility restoration planning traditionally focused on response to natural disasters and hazardous materials accidental releases. These plans now need to integrate response to terrorist actions. Therefore, plans must address a wide range of potential vulnerabilities. Similar types of broad remediation planning are needed for restoration of waste and hazardous material handling areas and facilities. There are strong similarities in damage results and remediation activities between unintentional and terrorist actions; however, the uncertainties associated with terrorist actions result in a re-evaluation of approaches to planning. Restoration of public facilities following a release of a hazardous material is inherently far more complex than in confined industrial settings and has many unique technical, economic, social, and political challenges. Therefore, they arguably involve a superset of drivers, concerns and public agencies compared to other restoration efforts. This superset of conditions increases complexity of interactions, reduces our knowledge of the initial conditions, and even condenses the timeline for restoration response. Therefore, evaluations of alternative restoration management approaches developed for responding to terrorist actions provide useful knowledge for large, complex waste management projects. Whereas present planning documents have substantial linearity in their organization, the 'adaptive management' paradigm provides a constructive parallel operations paradigm for restoration of facilities that anticipates and plans for uncertainty, multiple/simUltaneous public agency actions, and stakeholder participation. Adaptive management grew out of the need to manage and restore natural resources in highly complex and changing environments with limited knowledge about causal relationships and responses to restoration actions. Similarities between natural resource management and restoration of a facility and surrounding area(s) after a disruptive event suggest numerous advantages over preset linearly-structured plans by incorporating the flexibility and overlap of processes inherent in effective facility restoration. We discuss three restoration case studies (e.g., the Hart Senate Office Building anthrax restoration, Rocky Flats actinide remediation, and hurricane destruction restoration), that implement aspects of adaptive management but not a formal approach. We propose that more formal adoption of adaptive management principles could be a basis for more flexible standards to improve site-specific remediation plans under conditions of high uncertainty.

  14. Performance of a MEMS-base Adaptive Optics Optical Coherency Tomography System

    SciTech Connect (OSTI)

    Evans, J; Zadwadzki, R J; Jones, S; Olivier, S; Opkpodu, S; Werner, J S

    2008-01-16

    We have demonstrated that a microelectrical mechanical systems (MEMS) deformable mirror can be flattened to < 1 nm RMS within controllable spatial frequencies over a 9.2-mm aperture making it a viable option for high-contrast adaptive optics systems (also known as Extreme Adaptive Optics). The Extreme Adaptive Optics Testbed at UC Santa Cruz is being used to investigate and develop technologies for high-contrast imaging, especially wavefront control. A phase shifting diffraction interferometer (PSDI) measures wavefront errors with sub-nm precision and accuracy for metrology and wavefront control. Consistent flattening, required testing and characterization of the individual actuator response, including the effects of dead and low-response actuators. Stability and repeatability of the MEMS devices was also tested. An error budget for MEMS closed loop performance will summarize MEMS characterization.

  15. Mutations in global regulators lead to metabolic selection during adaptation to complex environments

    SciTech Connect (OSTI)

    Saxer, Gerda; Krepps, Michael D.; Merkley, Eric D.; Ansong, Charles; Deatherage Kaiser, Brooke L.; Valovska, Marie -Thérèse; Ristic, Nikola; Yeh, Ping T.; Prakash, Vittal P.; Leiser, Owen P.; Nakhleh, Luay; Gibbons, Henry S.; Kreuzer, Helen W.; Shamoo, Yousif; Matic, Ivan

    2014-12-11

    Adaptation to ecologically complex environments can provide insights into the evolutionary dynamics and functional constraints encountered by organisms during natural selection. Unlike adaptation to a single limiting resource, adaptation to a new environment with abundant and varied resources can be difficult to achieve by small incremental changes since many mutations are required to achieve even modest gains in fitness. Since changing complex environments are quite common in nature, we investigated how such an epistatic bottleneck can be avoided to allow rapid adaptation. We show that adaptive mutations arise repeatedly in independently evolved populations in the context of greatly increased genetic and phenotypic diversity. We go on to show that weak selection requiring substantial metabolic reprogramming can be readily achieved by mutations in the global response regulator arcA and the stress response regulator rpoS. We identified 46 unique single-nucleotide variants of arcA and 18 mutations in rpoS, nine of which resulted in stop codons or large deletions, suggesting that a subtle modulation of ArcA function and knockouts of rpoS are largely responsible for the metabolic shifts leading to adaptation. These mutations allow a higher order “metabolic selection” that eliminates epistatic bottlenecks, which could occur when many changes would be required. Proteomic and carbohydrate analysis of adapting E. coli populations revealed an up-regulation of enzymes associated with the TCA cycle and amino acid metabolism and an increase in the secretion of putrescine. The overall effect of adaptation across populations is to redirect and efficiently utilize uptake and catabolism of abundant amino acids. Concomitantly, there is a pronounced spread of more ecologically limited strains that results from specialization through metabolic erosion. Remarkably, the global regulators arcA and rpoS can provide a “one-step” mechanism of adaptation to a novel environment, which highlights the importance of global resource management as a powerful strategy to adaptation.

  16. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Suggests Role as Molecular Adapter Print Wednesday, 24 June 2009 00:00 To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known...

  17. Scalable Adaptive Multilevel Solvers for Multiphysics Problems

    SciTech Connect (OSTI)

    Xu, Jinchao

    2014-12-01

    In this project, we investigated adaptive, parallel, and multilevel methods for numerical modeling of various real-world applications, including Magnetohydrodynamics (MHD), complex fluids, Electromagnetism, Navier-Stokes equations, and reservoir simulation. First, we have designed improved mathematical models and numerical discretizaitons for viscoelastic fluids and MHD. Second, we have derived new a posteriori error estimators and extended the applicability of adaptivity to various problems. Third, we have developed multilevel solvers for solving scalar partial differential equations (PDEs) as well as coupled systems of PDEs, especially on unstructured grids. Moreover, we have integrated the study between adaptive method and multilevel methods, and made significant efforts and advances in adaptive multilevel methods of the multi-physics problems.

  18. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Structure Suggests Role as Molecular Adapter Protein Structure Suggests Role as Molecular Adapter Print Wednesday, 24 June 2009 00:00 To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known as the replisome. An essential step in replisome assembly is the loading of ring-shaped helicases (motor proteins) onto the separated strands of DNA. Dedicated ATP-fueled proteins regulate the loading; however, the mechanism by which these proteins

  19. Lactose-Inducible System for Metabolic Engineering of Clostridium ljungdahlii

    SciTech Connect (OSTI)

    Banerjee, A; Leang, C; Ueki, T; Nevin, KP; Lovley, DR

    2014-03-25

    The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic physiology of acetogenesis. In an attempt to expand the genetic toolbox for C. ljungdahlii, the possibility of adapting a lactose-inducible system for gene expression, previously reported for Clostridium perfringens, was investigated. The plasmid pAH2, originally developed for C. perfringens with a gusA reporter gene, functioned as an effective lactose-inducible system in C. ljungdahlii. Lactose induction of C. ljungdahlii containing pB1, in which the gene for the aldehyde/alcohol dehydrogenase AdhE1 was downstream of the lactose-inducible promoter, increased expression of adhE1 30-fold over the wild-type level, increasing ethanol production 1.5-fold, with a corresponding decrease in acetate production. Lactose-inducible expression of adhE1 in a strain in which adhE1 and the adhE1 homolog adhE2 had been deleted from the chromosome restored ethanol production to levels comparable to those in the wild-type strain. Inducing expression of adhE2 similarly failed to restore ethanol production, suggesting that adhE1 is the homolog responsible for ethanol production. Lactose-inducible expression of the four heterologous genes necessary to convert acetyl coenzyme A (acetyl-CoA) to acetone diverted ca. 60% of carbon flow to acetone production during growth on fructose, and 25% of carbon flow went to acetone when carbon monoxide was the electron donor. These studies demonstrate that the lactose-inducible system described here will be useful for redirecting carbon and electron flow for the biosynthesis of products more valuable than acetate. Furthermore, this tool should aid in optimizing microbial electrosynthesis and for basic studies on the physiology of acetogenesis.

  20. An adaptive approach to resource management

    SciTech Connect (OSTI)

    Lessard, G.

    1995-12-01

    A formal process of adaptive management will be required to maximize the benefits of any option for land and natural resource management and to achieve the long-term objective of ecosystem management. The process itself is straightforward and simple: new information is identified, evaluated, and a determination is made whether to adjust strategy or goals. Adaptive management is a continuing process of action-based planning, monitoring, researching and adjusting with the objective of improving the implementation and achieving the desired goals and outcomes. In this process goals and objectives are clearly stated, an initial hypothesis of ecosystem behavior is described, and monitoring is conducted to provide rapid feedback for redirection of management experiments. While the concept of adaptive management is relatively straightforward, applying it to complex management strategies requires answers to several critical questions. What new information should compel an adjustment to the management strategy? What threshold should trigger this adjustment? Who decides when and how to make adjustments? What are the definitions and thresholds of acceptable results? Adaptive ecosystem management depends on a continually evolving understanding of cause-and-effect relationships in both biological and social systems. The key features in an adaptive approach are: (1) An experimental design for implementation; (2) An explicit description of the system; (3) Well defined goals and objectives (4) Identification of critical uncertainties; (5) A monitoring and evaluation program; (6) An aggressive approach to learning; and (7) An adaptable structure.

  1. Annex IV Environmental Webinar: Adaptive Management in the Marine...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Annex IV Environmental Webinar: Adaptive Management in the Marine Renewable Energy Industry Annex IV Environmental Webinar: Adaptive Management in the Marine Renewable Energy ...

  2. Remarks on Grid Generation Equidistribution and Solution-Adaptation...

    Office of Scientific and Technical Information (OSTI)

    Remarks on Grid Generation Equidistribution and Solution-Adaptation. Citation Details In-Document Search Title: Remarks on Grid Generation Equidistribution and Solution-Adaptation. ...

  3. Anisoplanatism in adaptive optics systems due to pupil aberrations...

    Office of Scientific and Technical Information (OSTI)

    Conference: Anisoplanatism in adaptive optics systems due to pupil aberrations Citation Details In-Document Search Title: Anisoplanatism in adaptive optics systems due to pupil ...

  4. Petascale, Adaptive CFD (ALCF ESP Technical Report): ALCF-2 Early...

    Office of Scientific and Technical Information (OSTI)

    Petascale, Adaptive CFD (ALCF ESP Technical Report): ALCF-2 Early Science Program Technical Report Citation Details In-Document Search Title: Petascale, Adaptive CFD (ALCF ESP ...

  5. High Flux Microchannel Solar Receiver Development with Adaptive...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Flux Microchannel Solar Receiver Development with Adaptive Flow Control High Flux Microchannel Solar Receiver Development with Adaptive Flow Control This presentation was delivered ...

  6. Asia-Pacific Regional Climate Change Adaptation Assessment |...

    Open Energy Info (EERE)

    Pacific Regional Climate Change Adaptation Assessment Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Asia-Pacific Regional Climate Change Adaptation Assessment Agency...

  7. The Cost to Developing Countries of Adapting to Climate Change...

    Open Energy Info (EERE)

    Cost to Developing Countries of Adapting to Climate Change Jump to: navigation, search Tool Summary LAUNCH TOOL Name: The Cost to Developing Countries of Adapting to Climate Change...

  8. Burundi-National Adaptation Plan of Action to Climate Change...

    Open Energy Info (EERE)

    National Adaptation Plan of Action to Climate Change Jump to: navigation, search Name Burundi-National Adaptation Plan of Action to Climate Change AgencyCompany Organization...

  9. Ethiopia-Climate Change National Adaptation Programme of Action...

    Open Energy Info (EERE)

    Climate Change National Adaptation Programme of Action Jump to: navigation, search Name Ethiopia-Climate Change National Adaptation Programme of Action AgencyCompany Organization...

  10. A National Strategy for Adaptation to Climate Change | Open Energy...

    Open Energy Info (EERE)

    National Strategy for Adaptation to Climate Change Jump to: navigation, search Tool Summary LAUNCH TOOL Name: A National Strategy for Adaptation to Climate Change AgencyCompany...

  11. Impacts of Climate Change on Agriculture and Adaptation in Vietnam...

    Open Energy Info (EERE)

    Climate Change on Agriculture and Adaptation in Vietnam Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Impacts of Climate Change on Agriculture and Adaptation in...

  12. India-Vulnerability Assessment and Enhancing Adaptive Capacities...

    Open Energy Info (EERE)

    Vulnerability Assessment and Enhancing Adaptive Capacities to Climate Change Jump to: navigation, search Name India-Vulnerability Assessment and Enhancing Adaptive Capacities to...

  13. OECD-Private Sector Engagement in Adaptation to Climate Change...

    Open Energy Info (EERE)

    Private Sector Engagement in Adaptation to Climate Change Jump to: navigation, search Tool Summary LAUNCH TOOL Name: OECD-Private Sector Engagement in Adaptation to Climate Change...

  14. Adaptation to Climate Variability and Change: A Guidance Manual...

    Open Energy Info (EERE)

    Adaptation to Climate Variability and Change: A Guidance Manual for Development Planning Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Adaptation to Climate...

  15. Rwanda-National Adaptation Programs of Action to Climate Change...

    Open Energy Info (EERE)

    National Adaptation Programs of Action to Climate Change Jump to: navigation, search Name Rwanda-National Adaptation Programs of Action to Climate Change AgencyCompany...

  16. GIZ Sourcebook Module 5f: Adapting Urban Transport to Climate...

    Open Energy Info (EERE)

    f: Adapting Urban Transport to Climate Change Jump to: navigation, search Tool Summary LAUNCH TOOL Name: GIZ Sourcebook Module 5f: Adapting Urban Transport to Climate Change...

  17. Climate Change and the Los Alamos National Laboratory: The Adaptation...

    Energy Savers [EERE]

    Climate Change and the Los Alamos National Laboratory: The Adaptation Challenge The Los Alamos National Laboratory (LANL) has been adapting to climate change related impacts that ...

  18. Anisoplanatism in adaptive optics systems due to pupil aberrations...

    Office of Scientific and Technical Information (OSTI)

    Anisoplanatism in adaptive optics systems due to pupil aberrations Citation Details In-Document Search Title: Anisoplanatism in adaptive optics systems due to pupil aberrations You ...

  19. Optical Design for Extremely Large Telescope Adaptive Optics...

    Office of Scientific and Technical Information (OSTI)

    Large Telescope Adaptive Optics Systems Citation Details In-Document Search Title: Optical Design for Extremely Large Telescope Adaptive Optics Systems You are accessing a ...

  20. Adaptive Particle Filtering for Mode Tracking: A Shallow Ocean...

    Office of Scientific and Technical Information (OSTI)

    Adaptive Particle Filtering for Mode Tracking: A Shallow Ocean Application Citation Details In-Document Search Title: Adaptive Particle Filtering for Mode Tracking: A Shallow Ocean ...

  1. Electricity Market Complex Adaptive System | Open Energy Information

    Open Energy Info (EERE)

    Complex Adaptive System Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Electricity Market Complex Adaptive System AgencyCompany Organization: Argonne National...

  2. Kenya-Strengthening Adaptation and Resilience to Climate Change...

    Open Energy Info (EERE)

    Kenya-Strengthening Adaptation and Resilience to Climate Change(StARCK) (Redirected from Strengthening Adaptation and Resilience to Climate Change in Kenya (StARCK)) Jump to:...

  3. Verification and operation of adaptive materials in space.

    SciTech Connect (OSTI)

    Dargaville, Tim Richard; Elliott, Julie M.; Jones, Gary D.; Celina, Mathias Christopher

    2006-12-01

    Piezoelectric polymers based on polyvinylidene fluoride (PVDF) are of interest as smart materials for novel space-based telescope applications. Dimensional adjustments of adaptive thin polymer films are achieved via controlled charge deposition. Predicting their long-term performance requires a detailed understanding of the piezoelectric property changes that develop during space environmental exposure. The overall materials performance is governed by a combination of chemical and physical degradation processes occurring in low Earth orbit as established by our past laboratory-based materials performance experiments (see report SAND 2005-6846). Molecular changes are primarily induced via radiative damage, and physical damage from temperature and atomic oxygen exposure is evident as depoling, loss of orientation and surface erosion. The current project extension has allowed us to design and fabricate small experimental units to be exposed to low Earth orbit environments as part of the Materials International Space Station Experiments program. The space exposure of these piezoelectric polymers will verify the observed trends and their degradation pathways, and provide feedback on using piezoelectric polymer films in space. This will be the first time that PVDF-based adaptive polymer films will be operated and exposed to combined atomic oxygen, solar UV and temperature variations in an actual space environment. The experiments are designed to be fully autonomous, involving cyclic application of excitation voltages, sensitive film position sensors and remote data logging. This mission will provide critically needed feedback on the long-term performance and degradation of such materials, and ultimately the feasibility of large adaptive and low weight optical systems utilizing these polymers in space.

  4. Vulnerability and adaptation to severe weather events in the American southwest

    SciTech Connect (OSTI)

    Boero, Riccardo; Bianchini, Laura; Pasqualini, Donatella

    2015-05-04

    Climate change can induce changes in the frequency of severe weather events representing a threat to socio-economic development. It is thus of uttermost importance to understand how the vulnerability to the weather of local communities is determined and how adaptation public policies can be effectively put in place. We focused our empirical analysis on the American Southwest. Results show that, consistently with the predictions of an investment model, economic characteristics signaling local economic growth in the near future decrease the level of vulnerability. We also show that federal governments transfers and grants neither work to support recovery from and adaptation to weather events nor to distribute their costs over a broader tax base. Finally, we show that communities relying on municipal bonds to finance adaptation and recovery policies can benefit from local acknowledgment of the need for such policies and that they do not have to pay lenders a premium for the risk induced by weather events. In conclusion, our findings suggest that determinants of economic growth support lower vulnerability to the weather and increase options for financing adaptation and recovery policies, but also that only some communities are likely to benefit from those processes.

  5. Note: On-line weak signal detection via adaptive stochastic resonance

    SciTech Connect (OSTI)

    Lu, Siliang; He, Qingbo Kong, Fanrang

    2014-06-15

    We design an instrument with a novel embedded adaptive stochastic resonance (SR) algorithm that consists of a SR module and a digital zero crossing detection module for on-line weak signal detection in digital signal processing applications. The two modules are responsible for noise filtering and adaptive parameter configuration, respectively. The on-line weak signal detection can be stably achieved in seconds. The prototype instrument exhibits an advance of 20 dB averaged signal-to-noise ratio and 5 times averaged adjust R-square as compared to the input noisy signal, in considering different driving frequencies and noise levels.

  6. The Adaptive Multi-scale Simulation Infrastructure

    SciTech Connect (OSTI)

    Tobin, William R.

    2015-09-01

    The Adaptive Multi-scale Simulation Infrastructure (AMSI) is a set of libraries and tools developed to support the development, implementation, and execution of general multimodel simulations. Using a minimal set of simulation meta-data AMSI allows for minimally intrusive work to adapt existent single-scale simulations for use in multi-scale simulations. Support for dynamic runtime operations such as single- and multi-scale adaptive properties is a key focus of AMSI. Particular focus has been spent on the development on scale-sensitive load balancing operations to allow single-scale simulations incorporated into a multi-scale simulation using AMSI to use standard load-balancing operations without affecting the integrity of the overall multi-scale simulation.

  7. Load attenuating passively adaptive wind turbine blade

    DOE Patents [OSTI]

    Veers, Paul S.; Lobitz, Donald W.

    2003-01-01

    A method and apparatus for improving wind turbine performance by alleviating loads and controlling the rotor. The invention employs the use of a passively adaptive blade that senses the wind velocity or rotational speed, and accordingly modifies its aerodynamic configuration. The invention exploits the load mitigation prospects of a blade that twists toward feather as it bends. The invention includes passively adaptive wind turbine rotors or blades with currently preferred power control features. The apparatus is a composite fiber horizontal axis wind-turbine blade, in which a substantial majority of fibers in the blade skin are inclined at angles of between 15 and 30 degrees to the axis of the blade, to produces passive adaptive aeroelastic tailoring (bend-twist coupling) to alleviate loading without unduly jeopardizing performance.

  8. Load attenuating passively adaptive wind turbine blade

    DOE Patents [OSTI]

    Veers, Paul S.; Lobitz, Donald W.

    2003-01-07

    A method and apparatus for improving wind turbine performance by alleviating loads and controlling the rotor. The invention employs the use of a passively adaptive blade that senses the wind velocity or rotational speed, and accordingly modifies its aerodynamic configuration. The invention exploits the load mitigation prospects of a blade that twists toward feather as it bends. The invention includes passively adaptive wind turbine rotors or blades with currently preferred power control features. The apparatus is a composite fiber horizontal axis wind-turbine blade, in which a substantial majority of fibers in the blade skin are inclined at angles of between 15 and 30 degrees to the axis of the blade, to produces passive adaptive aeroelastic tailoring (bend-twist coupling) to alleviate loading without unduly jeopardizing performance.

  9. Arbitrary Lagrangian Eulerian Adaptive Mesh Refinement

    Energy Science and Technology Software Center (OSTI)

    2009-09-29

    This is a simulation code involving an ALE (arbitrary Lagrangian-Eulerian) hydrocode with AMR (adaptive mesh refinement) and pluggable physics packages for material strength, heat conduction, radiation diffusion, and laser ray tracing developed a LLNL, UCSD, and Berkeley Lab. The code is an extension of the open source SAMRAI (Structured Adaptive Mesh Refinement Application Interface) code/library. The code can be used in laser facilities such as the National Ignition Facility. The code is alsi being appliedmore » to slurry flow (landslides).« less

  10. U-197: Cisco Adaptive Security Appliances Denial of Service Vulnerability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 7: Cisco Adaptive Security Appliances Denial of Service Vulnerability U-197: Cisco Adaptive Security Appliances Denial of Service Vulnerability June 22, 2012 - 7:00am Addthis PROBLEM: A vulnerability has been reported in Cisco Adaptive Security Appliances (ASA), which can be exploited by malicious people to cause a DoS (Denial of Service). PLATFORM: Cisco Adaptive Security Appliance (ASA) 8.x Cisco ASA 5500 Series Adaptive Security Appliances ABSTRACT: The vulnerability

  11. Response Elements

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-07-11

    The Guide provides acceptable methods for meeting the requirement of DOE O 151.1C for response elements that respond or contribute to response as needed in an emergency. Supersedes DOE G 151.1-1, Volume 3-1, DOE G 151.1-1, Volume 3-2, DOE G 151.1-1, Volume 3-3, DOE G 151.1-1, Volume 3-4, DOE G 151.1-1, Volume 4-1, DOE G 151.1-1, Volume 4-2, DOE G 151.1-1, Volume 4-3, DOE G 151.1-1, Volume 4-4, DOE G 151.1-1, Volume 4-5, and DOE G 151.1-1, Volume 4-6.

  12. Departmental Response:

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 Departmental Response: Assessment of the Report of the SEAB Task Force on Nuclear Nonproliferation Introduction Despite many successful U.S. efforts in nuclear nonproliferation, daunting challenges remain. Some nations are pursuing nuclear weapons and others are expanding their nuclear arsenals; some stockpiles of nuclear weapons and nuclear-weapons-usable materials remain dangerously insecure; and rapidly changing technologies and greater availability of dual-use knowledge are increasing the

  13. Demand Response

    Energy Savers [EERE]

    Demand Response Assessment for Eastern Interconnection Youngsun Baek, Stanton W. Hadley, Rocio Martinez, Gbadebo Oladosu, Alexander M. Smith, Fran Li, Paul Leiby and Russell Lee Prepared for FY12 DOE-CERTS Transmission Reliability R&D Internal Program Review September 20, 2012 2 Managed by UT-Battelle for the U.S. Department of Energy DOE National Laboratory Studies Funded to Support FOA 63 * DOE set aside $20 million from transmission funding for national laboratory studies. * DOE

  14. Adaptive, full-spectrum solar energy system

    DOE Patents [OSTI]

    Muhs, Jeffrey D.; Earl, Dennis D.

    2003-08-05

    An adaptive full spectrum solar energy system having at least one hybrid solar concentrator, at least one hybrid luminaire, at least one hybrid photobioreactor, and a light distribution system operably connected to each hybrid solar concentrator, each hybrid luminaire, and each hybrid photobioreactor. A lighting control system operates each component.

  15. Adaptive control system for gas producing wells

    SciTech Connect (OSTI)

    Fedor, Pashchenko; Sergey, Gulyaev; Alexander, Pashchenko

    2015-03-10

    Optimal adaptive automatic control system for gas producing wells cluster is proposed intended for solving the problem of stabilization of the output gas pressure in the cluster at conditions of changing gas flow rate and changing parameters of the wells themselves, providing the maximum high resource of hardware elements of automation.

  16. Commercial & Industrial Demand Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    & Events Skip navigation links Smart Grid Demand Response Agricultural Residential Demand Response Commercial & Industrial Demand Response Cross-sector Demand Response...

  17. Cellular responses to environmental DNA damage

    SciTech Connect (OSTI)

    Not Available

    1994-08-01

    This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

  18. Adaptive method for electron bunch profile prediction

    SciTech Connect (OSTI)

    Scheinker, Alexander; Gessner, Spencer

    2015-10-15

    We report on an experiment performed at the Facility for Advanced Accelerator Experimental Tests (FACET) at SLAC National Accelerator Laboratory, in which a new adaptive control algorithm, one with known, bounded update rates, despite operating on analytically unknown cost functions, was utilized in order to provide quasi-real-time bunch property estimates of the electron beam. Multiple parameters, such as arbitrary rf phase settings and other time-varying accelerator properties, were simultaneously tuned in order to match a simulated bunch energy spectrum with a measured energy spectrum. The simple adaptive scheme was digitally implemented using matlab and the experimental physics and industrial control system. Thus, the main result is a nonintrusive, nondestructive, real-time diagnostic scheme for prediction of bunch profiles, as well as other beam parameters, the precise control of which are important for the plasma wakefield acceleration experiments being explored at FACET.

  19. Adaptive method for electron bunch profile prediction

    SciTech Connect (OSTI)

    Scheinker, Alexander; Gessner, Spencer

    2015-10-01

    We report on an experiment performed at the Facility for Advanced Accelerator Experimental Tests (FACET) at SLAC National Accelerator Laboratory, in which a new adaptive control algorithm, one with known, bounded update rates, despite operating on analytically unknown cost functions, was utilized in order to provide quasi-real-time bunch property estimates of the electron beam. Multiple parameters, such as arbitrary rf phase settings and other time-varying accelerator properties, were simultaneously tuned in order to match a simulated bunch energy spectrum with a measured energy spectrum. The simple adaptive scheme was digitally implemented using matlab and the experimental physics and industrial control system. The main result is a nonintrusive, nondestructive, real-time diagnostic scheme for prediction of bunch profiles, as well as other beam parameters, the precise control of which are important for the plasma wakefield acceleration experiments being explored at FACET. © 2015 authors. Published by the American Physical Society.

  20. Adaptation strategies for health impacts of climate change in Western Australia: Application of a Health Impact Assessment framework

    SciTech Connect (OSTI)

    Spickett, Jeffery T.; Brown, Helen L.; Katscherian, Dianne

    2011-04-15

    Climate change is one of the greatest challenges facing the globe and there is substantial evidence that this will result in a number of health impacts, regardless of the level of greenhouse gas mitigation. It is therefore apparent that a combined approach of mitigation and adaptation will be required to protect public health. While the importance of mitigation is recognised, this project focused on the role of adaptation strategies in addressing the potential health impacts of climate change. The nature and magnitude of these health impacts will be determined by a number of parameters that are dependent upon the location. Firstly, climate change will vary between regions. Secondly, the characteristics of each region in terms of population and the ability to adapt to changes will greatly influence the extent of the health impacts that are experienced now and into the future. Effective adaptation measures therefore need to be developed with these differences in mind. A Health Impact Assessment (HIA) framework was used to consider the implications of climate change on the health of the population of Western Australia (WA) and to develop a range of adaptive responses suited to WA. A broad range of stakeholders participated in the HIA process, providing informed input into developing an understanding of the potential health impacts and potential adaptation strategies from a diverse sector perspective. Potential health impacts were identified in relation to climate change predictions in WA in the year 2030. The risk associated with each of these impacts was assessed using a qualitative process that considered the consequences and the likelihood of the health impact occurring. Adaptations were then developed which could be used to mitigate the identified health impacts and provide responses which could be used by Government for future decision making. The periodic application of a HIA framework is seen as an ideal tool to develop appropriate adaptation strategies to address the potential health impacts of climate change.

  1. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Structure Suggests Role as Molecular Adapter Print To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known as the replisome. An essential step in replisome assembly is the loading of ring-shaped helicases (motor proteins) onto the separated strands of DNA. Dedicated ATP-fueled proteins regulate the loading; however, the mechanism by which these proteins recruit and deposit helicases has remained unclear. To better understand this

  2. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Structure Suggests Role as Molecular Adapter Print To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known as the replisome. An essential step in replisome assembly is the loading of ring-shaped helicases (motor proteins) onto the separated strands of DNA. Dedicated ATP-fueled proteins regulate the loading; however, the mechanism by which these proteins recruit and deposit helicases has remained unclear. To better understand this

  3. Protein Structure Suggests Role as Molecular Adapter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protein Structure Suggests Role as Molecular Adapter Print To split and copy DNA during replication, all cellular organisms use a multicomponent molecular machine known as the replisome. An essential step in replisome assembly is the loading of ring-shaped helicases (motor proteins) onto the separated strands of DNA. Dedicated ATP-fueled proteins regulate the loading; however, the mechanism by which these proteins recruit and deposit helicases has remained unclear. To better understand this

  4. Adaptive Management in the Marine Renewable Energy Industry Webinar...

    Office of Environmental Management (EM)

    Adaptive Management in the Marine Renewable Energy Industry Webinar Adaptive Management in the Marine Renewable Energy Industry Webinar December 10, 2015 8:30AM to 10:00AM PST As...

  5. Kenya-Strengthening Adaptation and Resilience to Climate Change...

    Open Energy Info (EERE)

    Kenya-Strengthening Adaptation and Resilience to Climate Change(StARCK) Jump to: navigation, search Name Strengthening Adaptation and Resilience to Climate Change in Kenya (StARCK)...

  6. The Importance of Traditional Ecological Knowledge in Adaptation Planning

    Broader source: Energy.gov [DOE]

    The National Adaptation Forum is hosting a webinar to focus on the importance and role of traditional ecological knowledge in adaptation planning at the local, regional, and national level.

  7. Vulnerability and adaptation to severe weather events in the American southwest

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Boero, Riccardo; Bianchini, Laura; Pasqualini, Donatella

    2015-05-04

    Climate change can induce changes in the frequency of severe weather events representing a threat to socio-economic development. It is thus of uttermost importance to understand how the vulnerability to the weather of local communities is determined and how adaptation public policies can be effectively put in place. We focused our empirical analysis on the American Southwest. Results show that, consistently with the predictions of an investment model, economic characteristics signaling local economic growth in the near future decrease the level of vulnerability. We also show that federal governments transfers and grants neither work to support recovery from and adaptationmore » to weather events nor to distribute their costs over a broader tax base. Finally, we show that communities relying on municipal bonds to finance adaptation and recovery policies can benefit from local acknowledgment of the need for such policies and that they do not have to pay lenders a premium for the risk induced by weather events. In conclusion, our findings suggest that determinants of economic growth support lower vulnerability to the weather and increase options for financing adaptation and recovery policies, but also that only some communities are likely to benefit from those processes.« less

  8. Communication adapter for use with a drilling component

    DOE Patents [OSTI]

    Hall, David R.; Pixton, David S.; Hall; Jr.; H. Tracy; Bradford, Kline; Rawle, Michael

    2007-04-03

    A communication adapter is disclosed that provides for removable attachment to a drilling component when the drilling component is not actively drilling and for communication with an integrated transmission system in the drilling component. The communication adapter comprises a data transmission coupler that facilitates communication between the drilling component and the adapter, a mechanical coupler that facilitates removable attachment of the adapter to the drilling component, and a data interface.

  9. Climate Change Adaptation/Resilience | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Climate Change Adaptation/Resilience Climate Change Adaptation/Resilience DOE facilities are located in all eight climate regions identified in the 2014 National Climate Assessment (as established by the U.S. Global Change Research Program), and are vulnerable to identified climate change impacts in those regions. To assist with establishing and maintaining an effective climate adaptation process, DOE is working to integrate climate adaptation concerns into all applicable DOE orders, policies,

  10. Optical Design for Extremely Large Telescope Adaptive Optics Systems

    Office of Scientific and Technical Information (OSTI)

    (Thesis/Dissertation) | SciTech Connect Thesis/Dissertation: Optical Design for Extremely Large Telescope Adaptive Optics Systems Citation Details In-Document Search Title: Optical Design for Extremely Large Telescope Adaptive Optics Systems Designing an adaptive optics (AO) system for extremely large telescopes (ELT's) will present new optical engineering challenges. Several of these challenges are addressed in this work, including first-order design of multi-conjugate adaptive optics

  11. BIA Request for Proposals for Climate Adaptation Grants for Tribes

    Broader source: Energy.gov [DOE]

    Download the Bureau of Indian Affairs Request for Proposals for Climate Adaptation Grants for Tribes, due November 29.

  12. Adapting technology to keep the national infrastructure safe and secure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Adapting technology to keep the national infrastructure safe and secure Community Connections: Your link to news and opportunities from Los Alamos National Laboratory Latest Issue:May 2016 all issues All Issues » submit Adapting technology to keep the national infrastructure safe and secure Identifying threats and responding to disasters September 1, 2013 Adapting Lab technology to help recover from tornados and other disasters Adapting Lab technology to help recover from tornados and other

  13. Bayesian Approaches to Adaptive Spatial Sampling

    Energy Science and Technology Software Center (OSTI)

    2006-01-23

    The purpose of this software is to support the design of spatial sampling data collection programs to delineate contamination footprints in response to an environmental contamination release.

  14. Mechanical Response of Thermoelectric Materials

    SciTech Connect (OSTI)

    Wereszczak, Andrew A.; Case, Eldon D.

    2015-05-01

    A sufficient mechanical response of thermoelectric materials (TEMats) to structural loadings is a prerequisite to the exploitation of any candidate TEMat's thermoelectric efficiency. If a TEMat is mechanically damaged or cracks from service-induced stresses, then its thermal and electrical functions can be compromised or even cease. Semiconductor TEMats tend to be quite brittle and have a high coefficient of thermal expansion; therefore, they can be quite susceptible to mechanical failure when subjected to operational thermal gradients. Because of this, sufficient mechanical response (vis-a-vis, mechanical properties) of any candidate TEMat must be achieved and sustained in the context of the service-induced stress state to which it is subjected. This report provides an overview of the mechanical responses of state-of-the-art TEMats; discusses the relevant properties that are associated with those responses and their measurement; and describes important, nonequilibrium phenomena that further complicate their use in thermoelectric devices. For reference purposes, the report also includes several appendixes that list published data on elastic properties and strengths of a variety of TEMats.

  15. Adaptable Computing Environment/Self-Assembling Software

    Energy Science and Technology Software Center (OSTI)

    2007-09-25

    Complex software applications are difficult to learn to use and to remember how to use. Further, the user has no control over the functionality available in a given application. The software we use can be created and modified only by a relatively small group of elite, highly skilled artisans known as programmers. "Normal users" are powerless to create and modify software themselves, because the tools for software development, designed by and for programmers, are amore » barrier to entry. This software, when completed, will be a user-adaptable computing environment in which the user is really in control of his/her own software, able to adapt the system, make new parts of the system interactive, and even modify the behavior of the system itself. Som key features of the basic environment that have been implemented are (a) books in bookcases, where all data is stored, (b) context-sensitive compass menus (compass, because the buttons are located in compass directions relative to the mouose cursor position), (c) importing tabular data and displaying it in a book, (d) light-weight table querying/sorting, (e) a Reach&Get capability (sort of a "smart" copy/paste that prevents the user from copying invalid data), and (f) a LogBook that automatically logs all user actions that change data or the system itself. To bootstrap toward full end-user adaptability, we implemented a set of development tools. With the development tools, compass menus can be made and customized.« less

  16. Adaptive powertrain control for plugin hybrid electric vehicles

    DOE Patents [OSTI]

    Kedar-Dongarkar, Gurunath; Weslati, Feisel

    2013-10-15

    A powertrain control system for a plugin hybrid electric vehicle. The system comprises an adaptive charge sustaining controller; at least one internal data source connected to the adaptive charge sustaining controller; and a memory connected to the adaptive charge sustaining controller for storing data generated by the at least one internal data source. The adaptive charge sustaining controller is operable to select an operating mode of the vehicle's powertrain along a given route based on programming generated from data stored in the memory associated with that route. Further described is a method of adaptively controlling operation of a plugin hybrid electric vehicle powertrain comprising identifying a route being traveled, activating stored adaptive charge sustaining mode programming for the identified route and controlling operation of the powertrain along the identified route by selecting from a plurality of operational modes based on the stored adaptive charge sustaining mode programming.

  17. Adaptive Dynamic Event Tree in RAVEN code

    SciTech Connect (OSTI)

    Alfonsi, Andrea; Rabiti, Cristian; Mandelli, Diego; Cogliati, Joshua Joseph; Kinoshita, Robert Arthur

    2014-11-01

    RAVEN is a software tool that is focused on performing statistical analysis of stochastic dynamic systems. RAVEN has been designed in a high modular and pluggable way in order to enable easy integration of different programming languages (i.e., C++, Python) and coupling with other applications (system codes). Among the several capabilities currently present in RAVEN, there are five different sampling strategies: Monte Carlo, Latin Hyper Cube, Grid, Adaptive and Dynamic Event Tree (DET) sampling methodologies. The scope of this paper is to present a new sampling approach, currently under definition and implementation: an evolution of the DET me

  18. Grid and basis adaptive polynomial chaos techniques for sensitivity and uncertainty analysis

    SciTech Connect (OSTI)

    Perk, Zoltn Gilli, Luca Lathouwers, Danny Kloosterman, Jan Leen

    2014-03-01

    The demand for accurate and computationally affordable sensitivity and uncertainty techniques is constantly on the rise and has become especially pressing in the nuclear field with the shift to Best Estimate Plus Uncertainty methodologies in the licensing of nuclear installations. Besides traditional, already well developed methods such as first order perturbation theory or Monte Carlo sampling Polynomial Chaos Expansion (PCE) has been given a growing emphasis in recent years due to its simple application and good performance. This paper presents new developments of the research done at TU Delft on such Polynomial Chaos (PC) techniques. Our work is focused on the Non-Intrusive Spectral Projection (NISP) approach and adaptive methods for building the PCE of responses of interest. Recent efforts resulted in a new adaptive sparse grid algorithm designed for estimating the PC coefficients. The algorithm is based on Gerstner's procedure for calculating multi-dimensional integrals but proves to be computationally significantly cheaper, while at the same it retains a similar accuracy as the original method. More importantly the issue of basis adaptivity has been investigated and two techniques have been implemented for constructing the sparse PCE of quantities of interest. Not using the traditional full PC basis set leads to further reduction in computational time since the high order grids necessary for accurately estimating the near zero expansion coefficients of polynomial basis vectors not needed in the PCE can be excluded from the calculation. Moreover the sparse PC representation of the response is easier to handle when used for sensitivity analysis or uncertainty propagation due to the smaller number of basis vectors. The developed grid and basis adaptive methods have been implemented in Matlab as the Fully Adaptive Non-Intrusive Spectral Projection (FANISP) algorithm and were tested on four analytical problems. These show consistent good performance both in terms of the accuracy of the resulting PC representation of quantities and the computational costs associated with constructing the sparse PCE. Basis adaptivity also seems to make the employment of PC techniques possible for problems with a higher number of input parameters (1520), alleviating a well known limitation of the traditional approach. The prospect of larger scale applicability and the simplicity of implementation makes such adaptive PC algorithms particularly appealing for the sensitivity and uncertainty analysis of complex systems and legacy codes.

  19. The Genomes of the Fungal Plant Pathogens Cladosporium fulvum and Dothistroma septosporum Reveal Adaptation to Different Hosts and Lifestyles But Also Signatures of Common Ancestry

    SciTech Connect (OSTI)

    de Wit, Pierre J. G. M.; van der Burgt, Ate; Okmen, Bilal; Stergiopoulos, Ioannis; Abd-Elsalam, Kamel A.; Aerts, Andrea L.; Bahkali, Ali H.; Beenen, Henriek G.; Chettri, Oranav; Cos, Murray P.; Datema, Erwin; de Vries, Ronald P.; DHillon, Braham; Ganley, Austen R.; Griffiths, Scott A.; Guo, Yanan; Gamelin, Richard C.; Henrissat, Bernard; Kabir, M. Shahjahan; Jashni, Mansoor Karimi; Kema, Gert; Klaubauf, Sylvia; Lapidus, Alla; Levasseur, Anthony; Lindquist, Erika; Mehrabi, Rahim; Ohm, Robin A.; Owen, Timothy J.; Salamov, Asaf; Schwelm, Arne; Schijlen, Elio; Sun, Hui; van den Burg, Harrold A.; van Burg, Roeland C. H. J.; Zhang, Shuguang; Goodwin, Stephen B.; Grigoriev, Igor V.; Collemare, Jerome; Bradshaw, Rosie E.

    2012-05-04

    We sequenced and compared the genomes of the Dothideomycete fungal plant pathogens Cladosporium fulvum (Cfu) (syn. Passalora fulva) and Dothistroma septosporum (Dse) that are closely related phylogenetically, but have different lifestyles and hosts. Although both fungi grow extracellularly in close contact with host mesophyll cells, Cfu is a biotroph infecting tomato, while Dse is a hemibiotroph infecting pine. The genomes of these fungi have a similar set of genes (70percent of gene content in both genomes are homologs), but differ significantly in size (Cfu >61.1-Mb; Dse 31.2-Mb), which is mainly due to the difference in repeat content (47.2percent in Cfu versus 3.2percent in Dse). Recent adaptation to different lifestyles and hosts is suggested by diverged sets of genes. Cfu contains an tomatinase gene that we predict might be required for detoxification of tomatine, while this gene is absent in Dse. Many genes encoding secreted proteins are unique to each species and the repeat-rich areas in Cfu are enriched for these species-specific genes. In contrast, conserved genes suggest common host ancestry. Homologs of Cfu effector genes, including Ecp2 and Avr4, are present in Dse and induce a Cf-Ecp2- and Cf-4-mediated hypersensitive response, respectively. Strikingly, genes involved in production of the toxin dothistromin, a likely virulence factor for Dse, are conserved in Cfu, but their expression differs markedly with essentially no expression by Cfu in planta. Likewise, Cfu has a carbohydrate-degrading enzyme catalog that is more similar to that of necrotrophs or hemibiotrophs and a larger pectinolytic gene arsenal than Dse, but many of these genes are not expressed in planta or are pseudogenized. Overall, comparison of their genomes suggests that these closely related plant pathogens had a common ancestral host but since adapted to different hosts and lifestyles by a combination of differentiated gene content, pseudogenization, and gene regulation.

  20. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Disorder-Induced Microscopic Magnetic Memory Print Wednesday, 26 October 2005 00:00 The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired magnetic properties. But as the density of magnetic disks climbs, the size of the magnetic domains responsible for storage must decrease, posing new challenges. Beautiful theories based on random microscopic disorder have been developed over

  1. Method for removing tilt control in adaptive optics systems

    DOE Patents [OSTI]

    Salmon, J.T.

    1998-04-28

    A new adaptive optics system and method of operation are disclosed, whereby the method removes tilt control, and includes the steps of using a steering mirror to steer a wavefront in the desired direction, for aiming an impinging aberrated light beam in the direction of a deformable mirror. The deformable mirror has its surface deformed selectively by means of a plurality of actuators, and compensates, at least partially, for existing aberrations in the light beam. The light beam is split into an output beam and a sample beam, and the sample beam is sampled using a wavefront sensor. The sampled signals are converted into corresponding electrical signals for driving a controller, which, in turn, drives the deformable mirror in a feedback loop in response to the sampled signals, for compensating for aberrations in the wavefront. To this purpose, a displacement error (gradient) of the wavefront is measured, and adjusted by a modified gain matrix, which satisfies the following equation: G{prime} = (I{minus}X(X{sup T} X){sup {minus}1}X{sup T})G(I{minus}A). 3 figs.

  2. Method for removing tilt control in adaptive optics systems

    DOE Patents [OSTI]

    Salmon, Joseph Thaddeus

    1998-01-01

    A new adaptive optics system and method of operation, whereby the method removes tilt control, and includes the steps of using a steering mirror to steer a wavefront in the desired direction, for aiming an impinging aberrated light beam in the direction of a deformable mirror. The deformable mirror has its surface deformed selectively by means of a plurality of actuators, and compensates, at least partially, for existing aberrations in the light beam. The light beam is split into an output beam and a sample beam, and the sample beam is sampled using a wavefront sensor. The sampled signals are converted into corresponding electrical signals for driving a controller, which, in turn, drives the deformable mirror in a feedback loop in response to the sampled signals, for compensating for aberrations in the wavefront. To this purpose, a displacement error (gradient) of the wavefront is measured, and adjusted by a modified gain matrix, which satisfies the following equation: G'=(I-X(X.sup.T X).sup.-1 X.sup.T)G(I-A)

  3. Adapter plate assembly for adjustable mounting of objects

    DOE Patents [OSTI]

    Blackburn, Robert S.

    1987-01-01

    An adapter plate and two locking discs are together affixed to an optic table with machine screws or bolts threaded into a fixed array of internally threaded holes provided in the table surface. The adapter plate preferably has two, and preferably parallel, elongated locating slots each freely receiving a portion of one of the locking discs for secure affixation of the adapter plate to the optic table. A plurality of threaded apertures provided in the adapter plate are available to attach optical mounts or other devices onto the adapter plate in an orientation not limited by the disposition of the array of threaded holes in the table surface. An axially aligned but radially offset hole through each locking disc receives a screw that tightens onto the table, such that prior to tightening of the screw the locking disc may rotate and translate within each locating slot of the adapter plate for maximum flexibility of the orientation thereof.

  4. Adapter plate assembly for adjustable mounting of objects

    DOE Patents [OSTI]

    Blackburn, R.S.

    1986-05-02

    An adapter plate and two locking discs are together affixed to an optic table with machine screws or bolts threaded into a fixed array of internally threaded holes provided in the table surface. The adapter plate preferably has two, and preferably parallel, elongated locating slots each freely receiving a portion of one of the locking discs for secure affixation of the adapter plate to the optic table. A plurality of threaded apertures provided in the adapter plate are available to attach optical mounts or other devices onto the adapter plate in an orientation not limited by the disposition of the array of threaded holes in the table surface. An axially aligned but radially offset hole through each locking disc receives a screw that tightens onto the table, such that prior to tightening of the screw the locking disc may rotate and translate within each locating slot of the adapter plate for maximum flexibility of the orientation thereof.

  5. Visualization Tools for Adaptive Mesh Refinement Data

    SciTech Connect (OSTI)

    Weber, Gunther H.; Beckner, Vincent E.; Childs, Hank; Ligocki,Terry J.; Miller, Mark C.; Van Straalen, Brian; Bethel, E. Wes

    2007-05-09

    Adaptive Mesh Refinement (AMR) is a highly effective method for simulations that span a large range of spatiotemporal scales, such as astrophysical simulations that must accommodate ranges from interstellar to sub-planetary. Most mainstream visualization tools still lack support for AMR as a first class data type and AMR code teams use custom built applications for AMR visualization. The Department of Energy's (DOE's) Science Discovery through Advanced Computing (SciDAC) Visualization and Analytics Center for Enabling Technologies (VACET) is currently working on extending VisIt, which is an open source visualization tool that accommodates AMR as a first-class data type. These efforts will bridge the gap between general-purpose visualization applications and highly specialized AMR visual analysis applications. Here, we give an overview of the state of the art in AMR visualization research and tools and describe how VisIt currently handles AMR data.

  6. Visualization of Scalar Adaptive Mesh Refinement Data

    SciTech Connect (OSTI)

    VACET; Weber, Gunther; Weber, Gunther H.; Beckner, Vince E.; Childs, Hank; Ligocki, Terry J.; Miller, Mark C.; Van Straalen, Brian; Bethel, E. Wes

    2007-12-06

    Adaptive Mesh Refinement (AMR) is a highly effective computation method for simulations that span a large range of spatiotemporal scales, such as astrophysical simulations, which must accommodate ranges from interstellar to sub-planetary. Most mainstream visualization tools still lack support for AMR grids as a first class data type and AMR code teams use custom built applications for AMR visualization. The Department of Energy's (DOE's) Science Discovery through Advanced Computing (SciDAC) Visualization and Analytics Center for Enabling Technologies (VACET) is currently working on extending VisIt, which is an open source visualization tool that accommodates AMR as a first-class data type. These efforts will bridge the gap between general-purpose visualization applications and highly specialized AMR visual analysis applications. Here, we give an overview of the state of the art in AMR scalar data visualization research.

  7. Adaptable radiation monitoring system and method

    DOE Patents [OSTI]

    Archer, Daniel E. (Livermore, CA); Beauchamp, Brock R. (San Ramon, CA); Mauger, G. Joseph (Livermore, CA); Nelson, Karl E. (Livermore, CA); Mercer, Michael B. (Manteca, CA); Pletcher, David C. (Sacramento, CA); Riot, Vincent J. (Berkeley, CA); Schek, James L. (Tracy, CA); Knapp, David A. (Livermore, CA)

    2006-06-20

    A portable radioactive-material detection system capable of detecting radioactive sources moving at high speeds. The system has at least one radiation detector capable of detecting gamma-radiation and coupled to an MCA capable of collecting spectral data in very small time bins of less than about 150 msec. A computer processor is connected to the MCA for determining from the spectral data if a triggering event has occurred. Spectral data is stored on a data storage device, and a power source supplies power to the detection system. Various configurations of the detection system may be adaptably arranged for various radiation detection scenarios. In a preferred embodiment, the computer processor operates as a server which receives spectral data from other networked detection systems, and communicates the collected data to a central data reporting system.

  8. Adaptive model training system and method

    DOE Patents [OSTI]

    Bickford, Randall L; Palnitkar, Rahul M

    2014-11-18

    An adaptive model training system and method for filtering asset operating data values acquired from a monitored asset for selectively choosing asset operating data values that meet at least one predefined criterion of good data quality while rejecting asset operating data values that fail to meet at least the one predefined criterion of good data quality; and recalibrating a previously trained or calibrated model having a learned scope of normal operation of the asset by utilizing the asset operating data values that meet at least the one predefined criterion of good data quality for adjusting the learned scope of normal operation of the asset for defining a recalibrated model having the adjusted learned scope of normal operation of the asset.

  9. Adaptive model training system and method

    DOE Patents [OSTI]

    Bickford, Randall L; Palnitkar, Rahul M; Lee, Vo

    2014-04-15

    An adaptive model training system and method for filtering asset operating data values acquired from a monitored asset for selectively choosing asset operating data values that meet at least one predefined criterion of good data quality while rejecting asset operating data values that fail to meet at least the one predefined criterion of good data quality; and recalibrating a previously trained or calibrated model having a learned scope of normal operation of the asset by utilizing the asset operating data values that meet at least the one predefined criterion of good data quality for adjusting the learned scope of normal operation of the asset for defining a recalibrated model having the adjusted learned scope of normal operation of the asset.

  10. Final Report - High Flux Microchannel Receiver Development with Adaptive

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Flow Control | Department of Energy High Flux Microchannel Receiver Development with Adaptive Flow Control Final Report - High Flux Microchannel Receiver Development with Adaptive Flow Control Awardee: Oregon State University Location: Corvallis, OR Subprogram: Concentrating Solar Power Funding Program: SunShot Concentrating Solar Power R&D Project: High Flux Microchannel Receiver Development with Adaptive Flow Control This project is focused on the demonstration of a microchannelbased

  11. Anisoplanatism in adaptive optics systems due to pupil aberrations

    Office of Scientific and Technical Information (OSTI)

    (Conference) | SciTech Connect Conference: Anisoplanatism in adaptive optics systems due to pupil aberrations Citation Details In-Document Search Title: Anisoplanatism in adaptive optics systems due to pupil aberrations Adaptive optics systems typically include an optical relay that simultaneously images the science field to be corrected and also a set of pupil planes conjugate to the deformable mirror of the system. Often, in the optical spaces where DM's are placed, the pupils are

  12. 2014 DOE Climate Change Adaptation Plan | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 DOE Climate Change Adaptation Plan 2014 DOE Climate Change Adaptation Plan Document presents the U.S. Department of Energy (DOE) 2014 plan for adapting to climate change. PDF icon doe_ccap_2014.pdf More Documents & Publications Executive Order -- Preparing the United States for the Impacts of Climate Change Executive Order 13653-Preparing the United States for the Impacts of Climate Change Climate Action Champions: Resilience and Equity

  13. Adapting Advances in Remediation Science to Long-Term Surveillance |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Adapting Advances in Remediation Science to Long-Term Surveillance Adapting Advances in Remediation Science to Long-Term Surveillance Paper and presentation presented at the Waste Managent 2006 Conference. February 26 through March 2, 2006, Tucson, Arizona. David Peterson PDF icon Adapting Advances in Remediation Science to Long-Term Surveillance More Documents & Publications Applied Science and Technology Task Order Fiscal Year 2009 Year-End Summary Report Long-Term

  14. Foreign DNA Capture during CRISPR-CAS Adaptive Immunity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Foreign DNA Capture during CRISPR-CAS Adaptive Immunity Foreign DNA Capture during CRISPR-CAS Adaptive Immunity Print Thursday, 21 January 2016 16:45 While we humans view bacteria as the enemy, bacteria have enemies too, for example, viruses. To protect themselves, bacteria have developed an adaptive-type immune system that revolves around a unit of DNA known as CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats. A CRISPR unit of DNA is made up of

  15. Climate Change Adaptation Technical Fact Sheet: Groundwater Remediation Systems

    Broader source: Energy.gov [DOE]

    A key component of the preliminary Superfund climate change adaptation action plan involves developing tools that can help project managers and other cleanup stakeholders to identify, prioritize...

  16. Sandia Energy - Structural and Mechanical Adaptive Rotor Technology...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Mechanical Adaptive Rotor Technology (SMART) Final Project Reports Published Home Renewable Energy Energy News Wind Energy News & Events Systems Analysis Structural and...

  17. Hands-on Energy Adaptation Toolkit (HEAT) | Open Energy Information

    Open Energy Info (EERE)

    Management Assistance Program of the World Bank Sector: Energy Focus Area: Renewable Energy Topics: Adaptation, Implementation, Pathways analysis Resource Type: Guidemanual...

  18. Structure and function of a serine carboxypeptidase adapted for...

    Office of Scientific and Technical Information (OSTI)

    Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7 Citation Details In-Document Search Title: Structure and ...

  19. ADAPT Elektronik GmbH | Open Energy Information

    Open Energy Info (EERE)

    GmbH Jump to: navigation, search Name: ADAPT Elektronik GmbH Place: Grobheubach, Germany Zip: 63920 Sector: Solar Product: Germany-based manufacturer of electronic connector...

  20. Output-Based Error Estimation and Adaptation for Uncertainty...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Output-Based Error Estimation and Adaptation for Uncertainty Quantification Isaac M. Asher and Krzysztof J. Fidkowski University of Michigan US National Congress on Computational...

  1. Climate Change Adaptation Technical Fact Sheet: Landfills and...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of potential climate change vulnerabilities and (2) presenting possible adaptation measures that may be considered to increase a remedy's resilience to climate change impacts. ...

  2. Eritrea-National Adaptation Programme of Action | Open Energy...

    Open Energy Info (EERE)

    analysis Website http:unfccc.intresourcedoc Country Eritrea UN Region Eastern Africa References Eritrea-National Adaptation Programme of Action1 This article is a...

  3. Enabling High Efficiency Low Temperature Combustion by Adaptive...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A new approach, called Adaptive-Jet-Cooling, leverages two distinct spray patters of hollow conical sprays and conventional multiple jets, eliminating key sources of NOx and PM. ...

  4. Adaptive Pitch Control for Variable Speed Wind Turbines - Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wind Energy Wind Energy Energy Analysis Energy Analysis Find More Like This Return to Search Adaptive Pitch Control for Variable Speed Wind Turbines National Renewable Energy ...

  5. Applying Climate Information for Adaptation Decision-Making:...

    Open Energy Info (EERE)

    climate change. About This document is intended for experts engaged in climate risk policy assessments and consultants advising the design and implementation of adaptation...

  6. Adaptive Stack with Subdivided Cells for Improved Stability,...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for Improved Stability, Reliability, and Durability under Automotive Load Cycle Adaptive Stack with Subdivided Cells for Improved Stability, Reliability, and Durability under ...

  7. A Posteriori Error Analysis and Adaptive Construction of Surrogate...

    Office of Scientific and Technical Information (OSTI)

    A Posteriori Error Analysis and Adaptive Construction of Surrogate Models. Citation ... Sponsoring Org: USDOE National Nuclear Security Administration (NNSA) Country of ...

  8. Economic Evaluation of Climate Change Adaptation Projects: Approaches...

    Open Energy Info (EERE)

    Evaluation of Climate Change Adaptation Projects: Approaches for the Agricultural Sector and Beyond Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Economic Evaluation...

  9. Adapting Urban Transport to Climate Change- Module 5f - Sustainable...

    Open Energy Info (EERE)

    Adapting Urban Transport to Climate Change- Module 5f - Sustainable transport: a sourcebook for policy-makers in developing cities Jump to: navigation, search Tool Summary LAUNCH...

  10. How to Integrate Climate Change Adaptation into National-Level...

    Open Energy Info (EERE)

    Integrate Climate Change Adaptation into National-Level Policy and Planning in the Water Sector Jump to: navigation, search Tool Summary LAUNCH TOOL Name: How to Integrate Climate...

  11. Development based climate change adaptation and mitigation-conceptual...

    Open Energy Info (EERE)

    based climate change adaptation and mitigation-conceptual issues and lessons learned in studies in developing countries Jump to: navigation, search Tool Summary LAUNCH TOOL Name:...

  12. Agricultural Technologies for Climate Change Mitigation and Adaptation...

    Open Energy Info (EERE)

    Technologies for Climate Change Mitigation and Adaptation in Developing Countries: Policy Options for Innovations and Technology Diffusion Jump to: navigation, search Tool Summary...

  13. Characterizing Uncertainty for Regional Climate Change Mitigation and Adaptation Decisions

    SciTech Connect (OSTI)

    Unwin, Stephen D.; Moss, Richard H.; Rice, Jennie S.; Scott, Michael J.

    2011-09-30

    This white paper describes the results of new research to develop an uncertainty characterization process to help address the challenges of regional climate change mitigation and adaptation decisions.

  14. Quantitative Adaptation Analytics for Assessing Dynamic Systems of Systems.

    SciTech Connect (OSTI)

    Gauthier, John H.; Miner, Nadine E.; Wilson, Michael L.; Le, Hai D.; Kao, Gio K; Melander, Darryl J.; Longsine, Dennis Earl; Vander Meer, Robert Charles,

    2015-01-01

    Our society is increasingly reliant on systems and interoperating collections of systems, known as systems of systems (SoS). These SoS are often subject to changing missions (e.g., nation- building, arms-control treaties), threats (e.g., asymmetric warfare, terrorism), natural environments (e.g., climate, weather, natural disasters) and budgets. How well can SoS adapt to these types of dynamic conditions? This report details the results of a three year Laboratory Directed Research and Development (LDRD) project aimed at developing metrics and methodologies for quantifying the adaptability of systems and SoS. Work products include: derivation of a set of adaptability metrics, a method for combining the metrics into a system of systems adaptability index (SoSAI) used to compare adaptability of SoS designs, development of a prototype dynamic SoS (proto-dSoS) simulation environment which provides the ability to investigate the validity of the adaptability metric set, and two test cases that evaluate the usefulness of a subset of the adaptability metrics and SoSAI for distinguishing good from poor adaptability in a SoS. Intellectual property results include three patents pending: A Method For Quantifying Relative System Adaptability, Method for Evaluating System Performance, and A Method for Determining Systems Re-Tasking.

  15. Adaptive Injection Strategies (AIS) for Ultra-low Emissions Diesel...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Injection Strategies (AIS) for Ultra-low Emissions Diesel Engines Adaptive Injection Strategies (AIS) for Ultra-low Emissions Diesel Engines Presentation given at the 2007 Diesel ...

  16. Rational design and adaptive management of combination therapies...

    Office of Scientific and Technical Information (OSTI)

    therapies for Hepatitis C virus infection Prev Next Title: Rational design and adaptive management of combination therapies for Hepatitis C virus infection Recent ...

  17. Rational Design and Adaptive Management of Combination Therapies...

    Office of Scientific and Technical Information (OSTI)

    of Combination Therapies for Hepatitis C Virus Infection CrossMark click for updates n ... Design and Adaptive Management of Combination Therapies for Hepatitis C Virus Infection. ...

  18. An adaptive wavelet stochastic collocation method for irregular...

    Office of Scientific and Technical Information (OSTI)

    Title: An adaptive wavelet stochastic collocation method for irregular solutions of ... This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and ...

  19. Africa Adaptation Programme: Capacity Building Experiences-Improving...

    Open Energy Info (EERE)

    Eastern Africa, Middle Africa, Northern Africa, Southern Africa, Western Africa Language: English Africa Adaptation Programme: Capacity Building Experiences-Improving Access,...

  20. Adaptive Wavenumber Estimation for Mode Tracking in a Shallow...

    Office of Scientific and Technical Information (OSTI)

    Tracking in a Shallow Ocean Environment Citation Details In-Document Search Title: Adaptive Wavenumber Estimation for Mode Tracking in a Shallow Ocean Environment You are ...

  1. A Shape Memory Polymer Dialysis Needle Adapter for the Reduction...

    Office of Scientific and Technical Information (OSTI)

    A Shape Memory Polymer Dialysis Needle Adapter for the Reduction of Hemodynamic Stress within Arteriovenous Grafts Citation Details In-Document Search Title: A Shape Memory Polymer ...

  2. Seismic Technology Adapted to Analyzing and Developing Geothermal...

    Open Energy Info (EERE)

    Technology Adapted to Analyzing and Developing Geothermal Systems Below Surface-Exposed High-Velocity Rocks Geothermal Project Jump to: navigation, search Last modified on July 22,...

  3. An adaptive wavelet stochastic collocation method for irregular...

    Office of Scientific and Technical Information (OSTI)

    adaptive (MdMrA) sparse grid stochastic collocation method, that utilizes hierarchical multiscale piecewise Riesz basis functions constructed from interpolating wavelets. ...

  4. UNDP-Adaptation Learning Mechanism | Open Energy Information

    Open Energy Info (EERE)

    Development Programme Sector Climate, Energy, Land, Water Topics Adaptation Resource Type Lessons learnedbest practices, Training materials Website http:www.adaptationlearning....

  5. An adaptive strategy to control anaerobic digesters for wastewater treatment

    SciTech Connect (OSTI)

    Monroy, O.; Alvarez-Ramirez, J.; Cuervo, F.; Femat, R.

    1996-10-01

    The design and implementation of a new adaptive controller for anaerobic digesters is presented using a general nonlinear model and an uncertainties estimation scheme. The primary advantage of this controller over standard adaptive controllers is that biogas flow rate measurements are not required. The resulting controller is similar in form to standard adaptive controllers and can be tuned analogously. The adaptive control strategy has been implemented in a pilot-scale anaerobic digester showing good performance and robustness against changes in the feed load.

  6. System-Response Issues Imposed by Biodiesel in a Medium-Duty...

    Broader source: Energy.gov (indexed) [DOE]

    More Documents & Publications Biodiesel's Enabling Characteristics in Attaining Low Temperature Diesel Combustion Fuel-Induced System Responses The Role Unconventional Fuels May ...

  7. The role of phase instabilities in the early material response...

    Office of Scientific and Technical Information (OSTI)

    The role of phase instabilities in the early material response during laser-induced breakdown in bulk fused silica Citation Details In-Document Search Title: The role of phase...

  8. 7th International Workshop on Microbeam Probes of Cellular Radiation Response

    SciTech Connect (OSTI)

    Brenner, David J.

    2009-07-21

    The extended abstracts that follow present a summary of the Proceedings of the 7th International Workshop: Microbeam Probes of Cellular Radiation Response, held at Columbia University’s Kellogg Center in New York City on March 15–17, 2006. These International Workshops on Microbeam Probes of Cellular Radiation Response have been held regularly since 1993 (1–5). Since the first workshop, there has been a rapid growth (see Fig. 1) in the number of centers developing microbeams for radiobiological research, and worldwide there are currently about 30 microbeams in operation or under development. Single-cell/single-particle microbeam systems can deliver beams of different ionizing radiations with a spatial resolution of a few micrometers down to a few tenths of a micrometer. Microbeams can be used to addressquestions relating to the effects of low doses of radiation (a single radiation track traversing a cell or group of cells), to probe subcellular targets (e.g. nucleus or cytoplasm), and to address questions regarding the propagation of information about DNA damage (for example, the radiation-induced bystander effect). Much of the recent research using microbeams has been to study low-dose effects and ‘‘non-targeted’’ responses such as bystander effects, genomic instability and adaptive responses. This Workshop provided a forum to assess the current state of microbeam technology and current biological applications and to discuss future directions for development, both technological and biological. Over 100 participants reviewed the current state of microbeam research worldwide and reported on new technological developments in the fields of both physics and biology.

  9. Low Dose IR Creates an Oncogenic Microenvironment by Inducing Premature

    SciTech Connect (OSTI)

    Yuan, Zhi-Min

    2013-04-28

    Introduction Much of the work addressing ionizing radiation-induced cellular response has been carried out mainly with the traditional cell culture technique involving only one cell type, how cellular response to IR is influenced by the tissue microenvironment remains elusive. By use of a three-dimensional (3D) co-culture system to model critical interactions of different cell types with their neighbors and with their environment, we recently showed that low-dose IR-induced extracellular signaling via the tissue environment affects profoundly cellular responses. This proposal aims at determining the response of mammary epithelial cells in a tissue-like setting.

  10. Rotatable superconducting cyclotron adapted for medical use

    DOE Patents [OSTI]

    Blosser, Henry G. (East Lansing, MI); Johnson, David A. (Williamston, MI); Riedel, Jack (East Lansing, MI); Burleigh, Richard J. (Berkeley, CA)

    1985-01-01

    A superconducting cyclotron (10) rotatable on a support structure (11) in an arc of about 180.degree. around a pivot axis (A--A) and particularly adapted for medical use is described. The rotatable support structure (13, 15) is balanced by being counterweighted (14) so as to allow rotation of the cyclotron and a beam (12), such as a subparticle (neutron) or atomic particle beam, from the cyclotron in the arc around a patient. Flexible hose (25) is moveably attached to the support structure for providing a liquified gas which is supercooled to near 0.degree. K. to an inlet means (122) to a chamber (105) around superconducting coils (101, 102). The liquid (34) level in the cyclotron is maintained approximately half full so that rotation of the support structure and cyclotron through the 180.degree. can be accomplished without spilling the liquid from the cyclotron. With the coils vertically oriented, each turn of the winding is approximately half immersed in liquid (34) and half exposed to cold gas and adequate cooling to maintain superconducting temperatures in the section of coil above the liquid level is provided by the combination of cold gas/vapor and by the conductive flow of heat along each turn of the winding from the half above the liquid to the half below.

  11. Modeling Power Systems as Complex Adaptive Systems

    SciTech Connect (OSTI)

    Chassin, David P.; Malard, Joel M.; Posse, Christian; Gangopadhyaya, Asim; Lu, Ning; Katipamula, Srinivas; Mallow, J V.

    2004-12-30

    Physical analogs have shown considerable promise for understanding the behavior of complex adaptive systems, including macroeconomics, biological systems, social networks, and electric power markets. Many of today's most challenging technical and policy questions can be reduced to a distributed economic control problem. Indeed, economically based control of large-scale systems is founded on the conjecture that the price-based regulation (e.g., auctions, markets) results in an optimal allocation of resources and emergent optimal system control. This report explores the state-of-the-art physical analogs for understanding the behavior of some econophysical systems and deriving stable and robust control strategies for using them. We review and discuss applications of some analytic methods based on a thermodynamic metaphor, according to which the interplay between system entropy and conservation laws gives rise to intuitive and governing global properties of complex systems that cannot be otherwise understood. We apply these methods to the question of how power markets can be expected to behave under a variety of conditions.

  12. Adaptive Analysis Engine v 1.0

    Energy Science and Technology Software Center (OSTI)

    2010-08-05

    This software provides an adaptable framework for performing comprehensive analyses of digital data streams. This software can be used to acquire and analyze raw digital data streams, as well as define, extract, and analyze data channels embedded in digital data streams.This software serves as a foundational framework for developing comprehensive software-based data acquisition, extraction, and analysis tools for ground-based, airborne, and space-based data stream sources. This software serves as a foundational framework for developing comprehensivemore » software-based data acquisition, extraction, and analysis tools for data stream sources from ground-based, airborne, and space-based data stream sources – primarily radio-frequency (RF) data links. Potential applications include state-of-health data links from communication satellites, aircraft, and vehicles, as well as stationary data collection platforms such as well monitors and weather stations. The software was developed with an abstract and extensible architecture which allows it to be easily configured to support any of these diverse data analysis applications« less

  13. A method for online verification of adapted fields using an independent dose monitor

    SciTech Connect (OSTI)

    Chang Jina; Norrlinger, Bernhard D.; Heaton, Robert K.; Jaffray, David A.; Cho, Young-Bin; Islam, Mohammad K.; Mahon, Robert

    2013-07-15

    Purpose: Clinical implementation of online adaptive radiotherapy requires generation of modified fields and a method of dosimetric verification in a short time. We present a method of treatment field modification to account for patient setup error, and an online method of verification using an independent monitoring system.Methods: The fields are modified by translating each multileaf collimator (MLC) defined aperture in the direction of the patient setup error, and magnifying to account for distance variation to the marked isocentre. A modified version of a previously reported online beam monitoring system, the integral quality monitoring (IQM) system, was investigated for validation of adapted fields. The system consists of a large area ion-chamber with a spatial gradient in electrode separation to provide a spatially sensitive signal for each beam segment, mounted below the MLC, and a calculation algorithm to predict the signal. IMRT plans of ten prostate patients have been modified in response to six randomly chosen setup errors in three orthogonal directions.Results: A total of approximately 49 beams for the modified fields were verified by the IQM system, of which 97% of measured IQM signal agree with the predicted value to within 2%.Conclusions: The modified IQM system was found to be suitable for online verification of adapted treatment fields.

  14. A phased approach to induced seismicity risk management

    SciTech Connect (OSTI)

    White, Joshua A.; Foxall, William

    2014-01-01

    This work describes strategies for assessing and managing induced seismicity risk during each phase of a carbon storage project. We consider both nuisance and damage potential from induced earthquakes, as well as the indirect risk of enhancing fault leakage pathways. A phased approach to seismicity management is proposed, in which operations are continuously adapted based on available information and an on-going estimate of risk. At each project stage, specific recommendations are made for (a) monitoring and characterization, (b) modeling and analysis, and (c) site operations. The resulting methodology can help lower seismic risk while ensuring site operations remain practical and cost-effective.

  15. A phased approach to induced seismicity risk management

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    White, Joshua A.; Foxall, William

    2014-01-01

    This work describes strategies for assessing and managing induced seismicity risk during each phase of a carbon storage project. We consider both nuisance and damage potential from induced earthquakes, as well as the indirect risk of enhancing fault leakage pathways. A phased approach to seismicity management is proposed, in which operations are continuously adapted based on available information and an on-going estimate of risk. At each project stage, specific recommendations are made for (a) monitoring and characterization, (b) modeling and analysis, and (c) site operations. The resulting methodology can help lower seismic risk while ensuring site operations remain practical andmore » cost-effective.« less

  16. Changes in expression of renal Oat1, Oat3 and Mrp2 in cisplatin-induced acute renal failure after treatment of JBP485 in rats

    SciTech Connect (OSTI)

    Liu, Tao, E-mail: liutaomedical@qq.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China)] [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Meng, Qiang, E-mail: mengq531@yahoo.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Wang, Changyuan, E-mail: wangcyuan@163.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Liu, Qi, E-mail: llaqii@yahoo.com.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Guo, Xinjin, E-mail: guo.xinjin@163.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China)] [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Sun, Huijun, E-mail: sunhuijun@hotmail.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Peng, Jinyong, E-mail: jinyongpeng2005@163.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); and others

    2012-11-01

    The purpose of this study is to investigate whether the effect of cyclo-trans-4-L-hydroxyprolyl-L-serine (JBP485) on acute renal failure (ARF) induced by cisplatin is related to change in expression of renal Oat1, Oat3 and Mrp2 in rats. JBP485 reduced creatinine, blood urea nitrogen (BUN) and indoxyl sulfate (IS) in plasma and malondialdehyde (MDA) in kidney, and recovered the glomerular filtration rate (GFR) and the activity of superoxide dismutase (SOD) in cisplatin-treated rats. The plasma concentration of PAH (para-aminohippurate) determined by LCMS/MS was increased markedly after intravenous administration of cisplatin, whereas cumulative urinary excretion of PAH and the uptake of PAH in kidney slices were significantly decreased. qRT-PCR and Western-blot showed a decrease in mRNA and protein of Oat1 and Oat3, an increase in mRNA and protein of Mrp2 in cisplatin-treated rats, and an increase in IS (a uremic toxin) after co-treatment with JBP485. It indicated that JBP485 promoted urinary excretion of toxins by upregulating renal Mrp2. This therefore gives in part the explanation about the mechanism by which JBP485 improves ARF induced by cisplatin in rats. -- Highlights: ? Cisplatin induces acute renal failure (ARF). ? The expression of Oat1, Oat3 and Mrp2 were changed during ARF. ? The regulated expression of Oat1, Oat3 and Mrp2 is an adaptive protected response. ? JBP485 could facilitate the adaptive protective action.

  17. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, Charlton; Subbaraman, Maria R.

    1989-01-01

    An improvement in an inducer for a pump wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs.

  18. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, C.; Subbaraman, M.R.

    1989-06-13

    An improvement in an inducer for a pump is disclosed wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs. 11 figs.

  19. Adaptive h -refinement for reduced-order models: ADAPTIVE h -refinement for reduced-order models

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Carlberg, Kevin T.

    2014-11-05

    Our work presents a method to adaptively refine reduced-order models a posteriori without requiring additional full-order-model solves. The technique is analogous to mesh-adaptive h-refinement: it enriches the reduced-basis space online by ‘splitting’ a given basis vector into several vectors with disjoint support. The splitting scheme is defined by a tree structure constructed offline via recursive k-means clustering of the state variables using snapshot data. This method identifies the vectors to split online using a dual-weighted-residual approach that aims to reduce error in an output quantity of interest. The resulting method generates a hierarchy of subspaces online without requiring large-scale operationsmore » or full-order-model solves. Furthermore, it enables the reduced-order model to satisfy any prescribed error tolerance regardless of its original fidelity, as a completely refined reduced-order model is mathematically equivalent to the original full-order model. Experiments on a parameterized inviscid Burgers equation highlight the ability of the method to capture phenomena (e.g., moving shocks) not contained in the span of the original reduced basis.« less

  20. Cross-sector Demand Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    & Events Skip navigation links Smart Grid Demand Response Agricultural Residential Demand Response Commercial & Industrial Demand Response Cross-sector Demand Response...

  1. Model-reference adaptive control applied to load-following of a space-nuclear power system

    SciTech Connect (OSTI)

    Metzger, J.D.; El-Genk, M.S.; Parlos, A.G.; New Mexico Univ., Albuquerque, NM . Inst. for Space Nuclear Power Studies; Texas A and M Univ., College Station, TX . Dept. of Nuclear Engineering)

    1989-01-01

    Nuclear power systems are presently being investigated as an alternative for both commercial and military space power systems because of their projected longevity of 7 to 10 years, their mass advantage over other space power sources at powers above approximately 25 kW{sub e}, and their ability to operate without direct illumination from the sun. These space-nuclear power systems are being designed to supply from tens of kilowatts to multimegawatts of power for continuous operation of seven years and more. Space-nuclear power systems designs that meet these requirements will not be available for refueling or maintenance during their lifetime. To ensure that the space-nuclear power system will operate safely and will respond in a predictable and desired manner, the design of the system's controller must account for changes in the system parameters over its lifetime. This paper applies model-reference adaptive control to an increase in the power demand by the load. A model-reference adaptive controller will force the actual space-nuclear power system to follow the predictable and desired response of a reference model, despite changes in the actual system's operating parameters. Included in this paper are the model-reference adaptive control algorithm, the description of the computer simulation of a space-nuclear power system and the reference model, and results that demonstrate the application of model-reference adaptive control to a change in the load power demand. The results demonstrate that model-reference adaptive control can ensure the transient response of the system despite differences between the design of the system and the as-built system as well as for variations in the systems parameters. 5 refs., 3 figs.

  2. Conformational Changes in Guanylate Cyclase-Activating Protein 1 Induced by Ca2+ and N-Terminal Fatty Acid Acylation

    SciTech Connect (OSTI)

    Orban, T.; Bereta, G; Miyagi, M; Wang, B; Chance, M; Sousa, M; Palczewski, K

    2010-01-01

    Neuronal Ca{sup 2+} sensors (NCS) are high-affinity Ca{sup 2+}-binding proteins critical for regulating a vast range of physiological processes. Guanylate cyclase-activating proteins (GCAPs) are members of the NCS family responsible for activating retinal guanylate cyclases (GCs) at low Ca{sup 2+} concentrations, triggering synthesis of cGMP and recovery of photoreceptor cells to the dark-adapted state. Here we use amide hydrogen-deuterium exchange and radiolytic labeling, and molecular dynamics simulations to study conformational changes induced by Ca{sup 2+} and modulated by the N-terminal myristoyl group. Our data on the conformational dynamics of GCAP1 in solution suggest that Ca{sup 2+} stabilizes the protein but induces relatively small changes in the domain structure; however, loss of Ca{sup 2+} mediates a significant global relaxation and movement of N- and C-terminal domains. This model and the previously described calcium-myristoyl switch proposed for recoverin indicate significant diversity in conformational changes among these highly homologous NCS proteins with distinct functions.

  3. Accelerating PDE-Constrained Optimization Problems using Adaptive...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Accelerating PDE-Constrained Optimization Problems using Adaptive Reduced-Order Models January 15, 2016 10:30AM to 11:30AM Presenter Matthew Zahr, Wilkinson Interviewee Location...

  4. Smoothed aggregation adaptive spectral element-based algebraic multigrid

    Energy Science and Technology Software Center (OSTI)

    2015-01-20

    SAAMGE provides parallel methods for building multilevel hierarchies and solvers that can be used for elliptic equations with highly heterogeneous coefficients. Additionally, hierarchy adaptation is implemented allowing solving multiple problems with close coefficients without rebuilding the hierarchy.

  5. Adaptive Nodal Transport Methods for Reactor Transient Analysis

    SciTech Connect (OSTI)

    Thomas Downar; E. Lewis

    2005-08-31

    Develop methods for adaptively treating the angular, spatial, and time dependence of the neutron flux in reactor transient analysis. These methods were demonstrated in the DOE transport nodal code VARIANT and the US NRC spatial kinetics code, PARCS.

  6. COLLOQUIUM: Adaptive Optics Imaging of Extosolar Planets | Princeton...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    April 23, 2015, 4:15pm to 5:30pm Colloquia MBG Auditorium COLLOQUIUM: Adaptive Optics Imaging of Extosolar Planets Professor Bruce MacIntosh Stanford University Presentation: PDF...

  7. Hell and High Water: Practice-Relevant Adaptation Science

    SciTech Connect (OSTI)

    Moss, Richard H.; Meehl, G.; Lemos, Maria Carmen; Smith, J. B.; Arnold, J. R.; Arnott, J. C.; Behar, D.; Brasseur, Guy P.; Broomell, S. B.; Busalacchi, Antonio; Dessai, S.; Ebi, Kristie L.; Edmonds, James A.; Furlow, J.; Goddard, L.; Hartmann, Holly; Hurrell, Jim; Katzenberger, J. W.; Liverman, D. M.; Mote, Phil; Moser, S. C.; Kumar, A.; Pulwarty, Roger; Seyller, E. A.; Turner, B.L.; Washington, Warren M.; Wilbanks, Thomas

    2013-11-08

    Recent extreme weather such as Hurricane Sandy and the 2012 drought demonstrate the vulnerability of the United States to climate extremes in the present and point to the potential for increased future damages under a changing climate. They also provide lessons for reducing harm and realizing any potential benefits. Preparedness measures also referred to as adaptation can cost-effectively increase resilience today and in the future. The upfront costs will be more than offset by reductions in property damage, lives and livelihoods lost, and expensive post-disaster recovery processes. While others have addressed use of science for adaptation in specific sectors including biodiversity (Heller and Zavaleta, 2009) and freshwater ecosystem management (Wilby et al., 2010), or by simply taking a more pragmatic approach to adaptation under uncertainty (Hallegatte, 2009), here the authors make the case that a new, comprehensive approach is needed to create and use science to inform adaptations with applicable and sound knowledge (Kerr et al., 2011).

  8. Climate Change Adaptation Technical Fact Sheet: Contaminated Sediment Remedies

    Broader source: Energy.gov [DOE]

    This fact sheet addresses remedies for contaminated sediment. It is intended to serve as an adaptation planning tool by (1) providing an overview of potential climate change vulnerabilities and (2)...

  9. Adaptive vibration control using synchronous demodulation with machine tool controller motor commutation

    DOE Patents [OSTI]

    Hopkins, David James

    2008-05-13

    A control system and method for actively reducing vibration in a spindle housing caused by unbalance forces on a rotating spindle, by measuring the force-induced spindle-housing motion, determining control signals based on synchronous demodulation, and provide compensation for the measured displacement to cancel or otherwise reduce or attenuate the vibration. In particular, the synchronous demodulation technique is performed to recover a measured spindle housing displacement signal related only to the rotation of a machine tool spindle, and consequently rejects measured displacement not related to spindle motion or synchronous to a cycle of revolution. Furthermore, the controller actuates at least one voice-coil (VC) motor, to cancel the original force-induced motion, and adapts the magnitude of voice coil signal until this measured displacement signal is brought to a null. In order to adjust the signal to a null, it must have the correct phase relative to the spindle angle. The feedback phase signal is used to adjust a common (to both outputs) commutation offset register (offset relative to spindle encoder angle) to force the feedback phase signal output to a null. Once both of these feedback signals are null, the system is compensating properly for the spindle-induced motion.

  10. Extreme Adaptive Optics for the Thirty Meter Telescope (Conference) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Extreme Adaptive Optics for the Thirty Meter Telescope Citation Details In-Document Search Title: Extreme Adaptive Optics for the Thirty Meter Telescope Direct detection of extrasolar Jovian planets is a major scientific motivation for the construction of future extremely large telescopes such as the Thirty Meter Telescope (TMT). Such detection will require dedicated high-contrast AO systems. Since the properties of Jovian planets and their parent stars vary enormously

  11. Generating code adapted for interlinking legacy scalar code and extended

    Office of Scientific and Technical Information (OSTI)

    vector code (Patent) | SciTech Connect Generating code adapted for interlinking legacy scalar code and extended vector code Citation Details In-Document Search Title: Generating code adapted for interlinking legacy scalar code and extended vector code Mechanisms for intermixing code are provided. Source code is received for compilation using an extended Application Binary Interface (ABI) that extends a legacy ABI and uses a different register configuration than the legacy ABI. First compiled

  12. TAP - Tools for Adaptive Partitioning v. 0.99 Beta

    Energy Science and Technology Software Center (OSTI)

    2008-11-19

    TAP is a set of tools which are essential for conducting research on adaptive partitioners. The basic premise is that a single partitioner may not be a good choice for adaptive mesh simulations; rather one must match a partitioner (obtained from a partitioning package like Zoltan, ParMetis etc) with the mesh being partitioned. TAP provides the tools that can judge the suitability of a partitioning algorithm to a given mesh.

  13. A Posteriori Error Analysis and Adaptive Construction of Surrogate Models.

    Office of Scientific and Technical Information (OSTI)

    (Conference) | SciTech Connect Posteriori Error Analysis and Adaptive Construction of Surrogate Models. Citation Details In-Document Search Title: A Posteriori Error Analysis and Adaptive Construction of Surrogate Models. Abstract not provided. Authors: Jakeman, John Davis ; Butler, Troy ; Constantine, Paul ; Dawson, Clint ; Bryant, Corey ; Prudhomme, Serge Publication Date: 2013-05-01 OSTI Identifier: 1080030 Report Number(s): SAND2013-4226C 455854 DOE Contract Number: AC04-94AL85000

  14. Adaptive Generation of Multimaterial Grids from imaging data for Biomedical

    Office of Scientific and Technical Information (OSTI)

    Lagrangian Fluid-Structure Simulations (Journal Article) | SciTech Connect Adaptive Generation of Multimaterial Grids from imaging data for Biomedical Lagrangian Fluid-Structure Simulations Citation Details In-Document Search Title: Adaptive Generation of Multimaterial Grids from imaging data for Biomedical Lagrangian Fluid-Structure Simulations Spatial discretization of complex imaging-derived fluid-solid geometries, such as the cardiac environment, is a critical but often overlooked

  15. Adaptive Particle Filtering for Mode Tracking: A Shallow Ocean Application

    Office of Scientific and Technical Information (OSTI)

    (Conference) | SciTech Connect Adaptive Particle Filtering for Mode Tracking: A Shallow Ocean Application Citation Details In-Document Search Title: Adaptive Particle Filtering for Mode Tracking: A Shallow Ocean Application Authors: Candy, J V Publication Date: 2011-07-12 OSTI Identifier: 1117989 Report Number(s): LLNL-CONF-491045 DOE Contract Number: W-7405-ENG-48 Resource Type: Conference Resource Relation: Conference: Presented at: OCEANS'11, Kona, HI, United States, Sep 19 - Sep 22, 2011

  16. Adaptive Wavenumber Estimation for Mode Tracking in a Shallow Ocean

    Office of Scientific and Technical Information (OSTI)

    Environment (Conference) | SciTech Connect Adaptive Wavenumber Estimation for Mode Tracking in a Shallow Ocean Environment Citation Details In-Document Search Title: Adaptive Wavenumber Estimation for Mode Tracking in a Shallow Ocean Environment Authors: Candy, J V Publication Date: 2012-06-28 OSTI Identifier: 1059076 Report Number(s): LLNL-CONF-563556 DOE Contract Number: W-7405-ENG-48 Resource Type: Conference Resource Relation: Conference: Presented at: IEEE OCEANS '12, Hampton Roads, VA,

  17. An Adaptive Wavelet Stochastic Collocation Method for Irregular Solutions

    Office of Scientific and Technical Information (OSTI)

    of PDEs with Random Input Data (Journal Article) | SciTech Connect An Adaptive Wavelet Stochastic Collocation Method for Irregular Solutions of PDEs with Random Input Data Citation Details In-Document Search Title: An Adaptive Wavelet Stochastic Collocation Method for Irregular Solutions of PDEs with Random Input Data Authors: Gunzburger, Max D [1] ; Webster, Clayton G [1] ; Zhang, Guannan [1] + Show Author Affiliations ORNL Publication Date: 2013-01-01 OSTI Identifier: 1096319 DOE Contract

  18. Parametrically Adaptive Wavenumber Processing for Mode Tracking in a

    Office of Scientific and Technical Information (OSTI)

    Shallow Ocean Environment (Conference) | SciTech Connect Parametrically Adaptive Wavenumber Processing for Mode Tracking in a Shallow Ocean Environment Citation Details In-Document Search Title: Parametrically Adaptive Wavenumber Processing for Mode Tracking in a Shallow Ocean Environment Authors: Candy, J V Publication Date: 2013-08-05 OSTI Identifier: 1096593 Report Number(s): LLNL-CONF-642197 DOE Contract Number: W-7405-ENG-48 Resource Type: Conference Resource Relation: Conference:

  19. Ultra-lightweight telescope with MEMS adaptive optic for distortion

    Office of Scientific and Technical Information (OSTI)

    correction. (Technical Report) | SciTech Connect Technical Report: Ultra-lightweight telescope with MEMS adaptive optic for distortion correction. Citation Details In-Document Search Title: Ultra-lightweight telescope with MEMS adaptive optic for distortion correction. Recent world events have underscored the need for a satellite based persistent global surveillance capability. To be useful, the satellite must be able to continuously monitor objects the size of a person anywhere on the globe

  20. Ultra-lightweight telescope with MEMS adaptive optic for distortion

    Office of Scientific and Technical Information (OSTI)

    correction. (Technical Report) | SciTech Connect Ultra-lightweight telescope with MEMS adaptive optic for distortion correction. Citation Details In-Document Search Title: Ultra-lightweight telescope with MEMS adaptive optic for distortion correction. × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional

  1. Adaptive Stack with Subdivided Cells for Improved Stability, Reliability,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Durability under Automotive Load Cycle | Department of Energy Adaptive Stack with Subdivided Cells for Improved Stability, Reliability, and Durability under Automotive Load Cycle Adaptive Stack with Subdivided Cells for Improved Stability, Reliability, and Durability under Automotive Load Cycle Part of a $100 million fuel cell award announced by DOE Secretary Bodman on Oct. 25, 2006. PDF icon 5_plugpwr.pdf More Documents & Publications Fuel Cell Kickoff Meeting Agenda Fuel Cell

  2. Adaptive sliding mode control for a class of chaotic systems

    SciTech Connect (OSTI)

    Farid, R.; Ibrahim, A.; Zalam, B.

    2015-03-30

    Chaos control here means to design a controller that is able to mitigating or eliminating the chaos behavior of nonlinear systems that experiencing such phenomenon. In this paper, an Adaptive Sliding Mode Controller (ASMC) is presented based on Lyapunov stability theory. The well known Chua's circuit is chosen to be our case study in this paper. The study shows the effectiveness of the proposed adaptive sliding mode controller.

  3. Spinning Reserve From Responsive Loads

    SciTech Connect (OSTI)

    Kirby, B.J.

    2003-04-08

    Responsive load is the most underutilized reliability resource available to the power system today. It is currently not used at all to provide spinning reserve. Historically there were good reasons for this, but recent technological advances in communications and controls have provided new capabilities and eliminated many of the old obstacles. North American Electric Reliability Council (NERC), Federal Energy Regulatory Commission (FERC), Northeast Power Coordinating Council (NPCC), New York State Reliability Council (NYSRC), and New York Independent System Operator (NYISO) rules are beginning to recognize these changes and are starting to encourage responsive load provision of reliability services. The Carrier ComfortChoice responsive thermostats provide an example of these technological advances. This is a technology aimed at reducing summer peak demand through central control of residential and small commercial air-conditioning loads. It is being utilized by Long Island Power Authority (LIPA), Consolidated Edison (ConEd), Southern California Edison (SCE), and San Diego Gas and Electric (SDG&E). The technology is capable of delivering even greater response in the faster spinning reserve time frame (while still providing peak reduction). Analysis of demand reduction testing results from LIPA during the summer of 2002 provides evidence to back up this claim. It also demonstrates that loads are different from generators and that the conventional wisdom, which advocates for starting with large loads as better ancillary service providers, is flawed. The tempting approach of incrementally adapting ancillary service requirements, which were established when generators were the only available resources, will not work. While it is easier for most generators to provide replacement power and non-spinning reserve (the slower response services) than it is to supply spinning reserve (the fastest service), the opposite is true for many loads. Also, there is more financial reward for supplying spinning reserve than for supplying the other reserve services as a result of the higher spinning reserve prices. The LIPAedge program (LIPA's demand reduction program using Carrier ComfortChoice thermostats) provides an opportunity to test the use of responsive load for spinning reserve. With potentially 75 MW of spinning reserve capability already installed, this test program can also make an important contribution to the capacity needs of Long Island during the summer of 2003. Testing could also be done at ConEd ({approx}30 MW), SCE ({approx}15 MW), and/or SDG&E ({approx}15 MW). This paper is divided into six chapters. Chapter 2 discusses the contingency reserve ancillary services, their functions in supporting power system reliability, and their technical requirements. It also discusses the policy and tariff requirements and attempts to distinguish between ones that are genuinely necessary and ones that are artifacts of the technologies that were historically used to provide the services. Chapter 3 discusses how responsive load could provide contingency reserves (especially spinning reserve) for the power system. Chapter 4 specifically discusses the Carrier ComfortChoice responsive thermostat technology, the LIPAedge experience with that technology, and how the technology could be used to supply spinning reserve. Chapter 5 discusses a number of unresolved issues and suggests areas for further research. Chapter 6 offers conclusions and recommendations.

  4. A Strategy to Engage the Private Sector in Climate Change Adaptation...

    Open Energy Info (EERE)

    to Engage the Private Sector in Climate Change Adaptation in Bangladesh Jump to: navigation, search Name A Strategy to Engage the Private Sector in Climate Change Adaptation in...

  5. U.S. Department of Energy Climate Change Adaptation Policy Statement...

    Energy Savers [EERE]

    Climate Change Adaptation Policy Statement U.S. Department of Energy Climate Change Adaptation Policy Statement U.S. Department of Energy statement that commits the agency to ...

  6. T-631: Cisco XR 12000 Series Shared Port Adapters Interface Processor...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    31: Cisco XR 12000 Series Shared Port Adapters Interface Processor Vulnerability T-631: Cisco XR 12000 Series Shared Port Adapters Interface Processor Vulnerability May 26, 2011 - ...

  7. U.S. Department of Energy Climate Change Adaptation Policy Statement...

    Office of Environmental Management (EM)

    Climate Change Adaptation Policy Statement U.S. Department of Energy Climate Change Adaptation Policy Statement U.S. Department of Energy statement that commits the agency to...

  8. A variable DNA recognition site organization establishes the LiaR-mediated cell envelope stress response of enterococci to daptomycin

    SciTech Connect (OSTI)

    Davlieva, Milya; Shi, Yiwen; Leonard, Paul G.; Johnson, Troy A.; Zianni, Michael R.; Arias, Cesar A.; Ladbury, John E.; Shamoo, Yousif

    2015-04-19

    LiaR is a ‘master regulator’ of the cell envelope stress response in enterococci and many other Gram-positive organisms. Mutations to liaR can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. LiaR is phosphorylated in response to membrane stress to regulate downstream target operons. Using DNA footprinting of the regions upstream of the liaXYZ and liaFSR operons we show that LiaR binds an extended stretch of DNA that extends beyond the proposed canonical consensus sequence suggesting a more complex level of regulatory control of target operons. We go on to determine the biochemical and structural basis for increased resistance to daptomycin by the adaptive mutation to LiaR (D191N) first identified from the pathogen Enterococcus faecalis S613. LiaRD191N increases oligomerization of LiaR to form a constitutively activated tetramer that has high affinity for DNA even in the absence of phosphorylation leading to increased resistance. The crystal structures of the LiaR DNA binding domain complexed to the putative consensus sequence as well as an adjoining secondary sequence show that upon binding, LiaR induces DNA bending that is consistent with increased recruitment of RNA polymerase to the transcription start site and upregulation of target operons.

  9. A variable DNA recognition site organization establishes the LiaR-mediated cell envelope stress response of enterococci to daptomycin

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Davlieva, Milya; Shi, Yiwen; Leonard, Paul G.; Johnson, Troy A.; Zianni, Michael R.; Arias, Cesar A.; Ladbury, John E.; Shamoo, Yousif

    2015-04-19

    LiaR is a ‘master regulator’ of the cell envelope stress response in enterococci and many other Gram-positive organisms. Mutations to liaR can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. LiaR is phosphorylated in response to membrane stress to regulate downstream target operons. Using DNA footprinting of the regions upstream of the liaXYZ and liaFSR operons we show that LiaR binds an extended stretch of DNA that extends beyond the proposed canonical consensus sequence suggesting a more complex level of regulatory control of target operons. We go on to determine the biochemical and structuralmore » basis for increased resistance to daptomycin by the adaptive mutation to LiaR (D191N) first identified from the pathogen Enterococcus faecalis S613. LiaRD191N increases oligomerization of LiaR to form a constitutively activated tetramer that has high affinity for DNA even in the absence of phosphorylation leading to increased resistance. The crystal structures of the LiaR DNA binding domain complexed to the putative consensus sequence as well as an adjoining secondary sequence show that upon binding, LiaR induces DNA bending that is consistent with increased recruitment of RNA polymerase to the transcription start site and upregulation of target operons.« less

  10. Comparative study of microwave radiation-induced magnetoresistive oscillations induced by circularly- and linearly- polarized photo-excitation

    SciTech Connect (OSTI)

    Ye, Tianyu; Liu, Han -Chun; Wang, Zhuo; Wegscheider, W.; Mani, Ramesh G.

    2015-10-09

    A comparative study of the radiation-induced magnetoresistance oscillations in the high mobility GaAs/AlGaAs heterostructure two dimensional electron system (2DES) under linearly- and circularly- polarized microwave excitation indicates a profound difference in the response observed upon rotating the microwave launcher for the two cases, although circularly polarized microwave radiation induced magnetoresistance oscillations observed at low magnetic fields are similar to the oscillations observed with linearly polarized radiation. For the linearly polarized radiation, the magnetoresistive response is a strong sinusoidal function of the launcher rotation (or linear polarization) angle, θ. As a result, for circularly polarized radiation, the oscillatory magnetoresistive response is hardly sensitive to θ.

  11. Thermoacoustic wave propagation modeling using a dynamically adaptive wavelet collocation method

    SciTech Connect (OSTI)

    Vasilyev, O.V.; Paolucci, S.

    1996-12-31

    When a localized region of a solid wall surrounding a compressible medium is subjected to a sudden temperature change, the medium in the immediate neighborhood of that region expands. This expansion generates pressure waves. These thermally-generated waves are referred to as thermoacoustic (TAC) waves. The main interest in thermoacoustic waves is motivated by their property to enhance heat transfer by inducing convective motion away from the heated area. Thermoacoustic wave propagation in a two-dimensional rectangular cavity is studied numerically. The thermoacoustic waves are generated by raising the temperature locally at the walls. The waves, which decay at large time due to thermal and viscous diffusion, propagate and reflect from the walls creating complicated two-dimensional patterns. The accuracy of numerical simulation is ensured by using a highly accurate, dynamically adaptive, multilevel wavelet collocation method, which allows local refinements to adapt to local changes in solution scales. Subsequently, high resolution computations are performed only in regions of large gradients. The computational cost of the method is independent of the dimensionality of the problem and is O(N), where N is the total number of collation points.

  12. Adapting Dry Cask Storage for Aging at a Geologic Repository

    SciTech Connect (OSTI)

    C. Sanders; D. Kimball

    2005-08-02

    A Spent Nuclear Fuel (SNF) Aging System is a crucial part of operations at the proposed Yucca Mountain repository in the United States. Incoming commercial SNF that does not meet thermal limits for emplacement will be aged on outdoor pads. U.S. Department of Energy SNF will also be managed using the Aging System. Proposed site-specific designs for the Aging System are closely based upon designs for existing dry cask storage (DCS) systems. This paper evaluates the applicability of existing DCS systems for use in the SNF Aging System at Yucca Mountain. The most important difference between existing DCS facilities and the Yucca Mountain facility is the required capacity. Existing DCS facilities typically have less than 50 casks. The current design for the aging pad at Yucca Mountain calls for a capacity of over 2,000 casks (20,000 MTHM) [1]. This unprecedented number of casks poses some unique problems. The response of DCS systems to off-normal and accident conditions needs to be re-evaluated for multiple storage casks. Dose calculations become more complicated, since doses from multiple or very long arrays of casks can dramatically increase the total boundary dose. For occupational doses, the geometry of the cask arrays and the order of loading casks must be carefully considered in order to meet ALARA goals during cask retrieval. Due to the large area of the aging pad, skyshine must also be included when calculating public and worker doses. The expected length of aging will also necessitate some design adjustments. Under 10 CFR 72.236, DCS systems are initially certified for a period of 20 years [2]. Although the Yucca Mountain facility is not intended to be a storage facility under 10 CFR 72, the operational life of the SNF Aging System is 50 years [1]. Any cask system selected for use in aging will have to be qualified to this design lifetime. These considerations are examined, and a summary is provided of the adaptations that must be made in order to use DCS technologies successfully at a geologic repository.

  13. Emergency Response Synchronization Matrix

    Energy Science and Technology Software Center (OSTI)

    1999-06-01

    An emergency response to a disaster is complex, requiring the rapid integration, coordination, and synchronization of multiple levels of governmental and non-governmental organizations from numerous jurisdictions into a unified community response. For example, a community’s response actions to a fixed site hazardous materials incident could occur in an area extending from an on-site storage location to points 25 or more miles away. Response actions are directed and controlled by local governments and agencies situated withinmore » the response area, as well as by state and federal operaticns centers quite removed from the area of impact. Time is critical and the protective action decision-making process is greatly compressed. The response community must carefully plan and coordinate response operations in order to have confidence that they will be effectively implemented when faced with the potentially catastrophic nature of such releases. A graphical depiction of the entire response process via an emergency response synchronization matrix is an effective tool in optimizing the planning, exercising, and implementation of emergency plans. This system—based approach to emergency planning depicts how a community organizes its response tasks across space and time in relation to hazard actions. It provides the opportunity to make real—time adjustments as necessary for maximizing the often limited resources in protecting area residents. A response must involve the entire community and must not be limited by individual jurisdictions and organizations acting on their own without coordination, integration, and synchronization.« less

  14. Climate variability and climate change vulnerability and adaptation. Workshop summary

    SciTech Connect (OSTI)

    Bhatti, N.; Cirillo, R.R.; Dixon, R.K.

    1995-12-31

    Representatives from fifteen countries met in Prague, Czech Republic, on September 11-15, 1995, to share results from the analysis of vulnerability and adaptation to global climate change. The workshop focused on the issues of global climate change and its impacts on various sectors of a national economy. The U.N. Framework Convention on Climate Change (FCCC), which has been signed by more than 150 governments worldwide, calls on signatory parties to develop and communicate measures they are implementing to respond to global climate change. An analysis of a country`s vulnerability to changes in the climate helps it identify suitable adaptation measures. These analyses are designed to determine the extent of the impacts of global climate change on sensitive sectors such as agricultural crops, forests, grasslands and livestock, water resources, and coastal areas. Once it is determined how vulnerable a country may be to climate change, it is possible to identify adaptation measures for ameliorating some or all of the effects.The objectives of the vulnerability and adaptation workshop were to: The objectives of the vulnerability and adaptation workshop were to: Provide an opportunity for countries to describe their study results; Encourage countries to learn from the experience of the more complete assessments and adjust their studies accordingly; Identify issues and analyses that require further investigation; and Summarize results and experiences for governmental and intergovernmental organizations.

  15. Adaptations in Electronic Structure Calculations in Heterogeneous Environments

    SciTech Connect (OSTI)

    Talamudupula, Sai

    2011-11-29

    Modern quantum chemistry deals with electronic structure calculations of unprecedented complexity and accuracy. They demand full power of high-performance computing and must be in tune with the given architecture for superior e#14;ciency. To make such applications resourceaware, it is desirable to enable their static and dynamic adaptations using some external software (middleware), which may monitor both system availability and application needs, rather than mix science with system-related calls inside the application. The present work investigates scienti#12;c application interlinking with middleware based on the example of the computational chemistry package GAMESS and middleware NICAN. The existing synchronous model is limited by the possible delays due to the middleware processing time under the sustainable runtime system conditions. Proposed asynchronous and hybrid models aim at overcoming this limitation. When linked with NICAN, the fragment molecular orbital (FMO) method is capable of adapting statically and dynamically its fragment scheduling policy based on the computing platform conditions. Signi#12;cant execution time and throughput gains have been obtained due to such static adaptations when the compute nodes have very di#11;erent core counts. Dynamic adaptations are based on the main memory availability at run time. NICAN prompts FMO to postpone scheduling certain fragments, if there is not enough memory for their immediate execution. Hence, FMO may be able to complete the calculations whereas without such adaptations it aborts.

  16. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Print The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired magnetic properties. But as the density of magnetic disks climbs, the size of the magnetic domains responsible for storage must decrease, posing new challenges. Beautiful theories based on random microscopic disorder have been developed over the past ten years. To directly compare these theories with precise experiments,

  17. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Print The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired magnetic properties. But as the density of magnetic disks climbs, the size of the magnetic domains responsible for storage must decrease, posing new challenges. Beautiful theories based on random microscopic disorder have been developed over the past ten years. To directly compare these theories with precise experiments,

  18. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Print The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired magnetic properties. But as the density of magnetic disks climbs, the size of the magnetic domains responsible for storage must decrease, posing new challenges. Beautiful theories based on random microscopic disorder have been developed over the past ten years. To directly compare these theories with precise experiments,

  19. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Print The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired magnetic properties. But as the density of magnetic disks climbs, the size of the magnetic domains responsible for storage must decrease, posing new challenges. Beautiful theories based on random microscopic disorder have been developed over the past ten years. To directly compare these theories with precise experiments,

  20. Adaptively Reevaluated Bayesian Localization (ARBL): A Novel Technique for Radiological Source Localization

    SciTech Connect (OSTI)

    Miller, Erin A.; Robinson, Sean M.; Anderson, Kevin K.; McCall, Jonathon D.; Prinke, Amanda M.; Webster, Jennifer B.; Seifert, Carolyn E.

    2015-06-01

    Adaptively Reevaluated Bayesian Localization (ARBL): A Novel Technique for Radiological Source Localization

  1. An Adaptive Unified Differential Evolution Algorithm for Global Optimization

    SciTech Connect (OSTI)

    Qiang, Ji; Mitchell, Chad

    2014-11-03

    In this paper, we propose a new adaptive unified differential evolution algorithm for single-objective global optimization. Instead of the multiple mutation strate- gies proposed in conventional differential evolution algorithms, this algorithm employs a single equation unifying multiple strategies into one expression. It has the virtue of mathematical simplicity and also provides users the flexibility for broader exploration of the space of mutation operators. By making all control parameters in the proposed algorithm self-adaptively evolve during the process of optimization, it frees the application users from the burden of choosing appro- priate control parameters and also improves the performance of the algorithm. In numerical tests using thirteen basic unimodal and multimodal functions, the proposed adaptive unified algorithm shows promising performance in compari- son to several conventional differential evolution algorithms.

  2. Amplitude variations on the Extreme Adaptive Optics testbed

    SciTech Connect (OSTI)

    Evans, J; Thomas, S; Dillon, D; Gavel, D; Phillion, D; Macintosh, B

    2007-08-14

    High-contrast adaptive optics systems, such as those needed to image extrasolar planets, are known to require excellent wavefront control and diffraction suppression. At the Laboratory for Adaptive Optics on the Extreme Adaptive Optics testbed, we have already demonstrated wavefront control of better than 1 nm rms within controllable spatial frequencies. Corresponding contrast measurements, however, are limited by amplitude variations, including those introduced by the micro-electrical-mechanical-systems (MEMS) deformable mirror. Results from experimental measurements and wave optic simulations of amplitude variations on the ExAO testbed are presented. We find systematic intensity variations of about 2% rms, and intensity variations with the MEMS to be 6%. Some errors are introduced by phase and amplitude mixing because the MEMS is not conjugate to the pupil, but independent measurements of MEMS reflectivity suggest that some error is introduced by small non-uniformities in the reflectivity.

  3. Data Assimilation in the ADAPT Photospheric Flux Transport Model

    SciTech Connect (OSTI)

    Hickmann, Kyle S.; Godinez, Humberto C.; Henney, Carl J.; Arge, C. Nick

    2015-03-17

    Global maps of the solar photospheric magnetic flux are fundamental drivers for simulations of the corona and solar wind and therefore are important predictors of geoeffective events. However, observations of the solar photosphere are only made intermittently over approximately half of the solar surface. The Air Force Data Assimilative Photospheric Flux Transport (ADAPT) model uses localized ensemble Kalman filtering techniques to adjust a set of photospheric simulations to agree with the available observations. At the same time, this information is propagated to areas of the simulation that have not been observed. ADAPT implements a local ensemble transform Kalman filter (LETKF) to accomplish data assimilation, allowing the covariance structure of the flux-transport model to influence assimilation of photosphere observations while eliminating spurious correlations between ensemble members arising from a limited ensemble size. We give a detailed account of the implementation of the LETKF into ADAPT. Advantages of the LETKF scheme over previously implemented assimilation methods are highlighted.

  4. GADRAS Detector Response Function.

    SciTech Connect (OSTI)

    Mitchell, Dean J.; Harding, Lee; Thoreson, Gregory G; Horne, Steven M.

    2014-11-01

    The Gamma Detector Response and Analysis Software (GADRAS) applies a Detector Response Function (DRF) to compute the output of gamma-ray and neutron detectors when they are exposed to radiation sources. The DRF is fundamental to the ability to perform forward calculations (i.e., computation of the response of a detector to a known source), as well as the ability to analyze spectra to deduce the types and quantities of radioactive material to which the detectors are exposed. This document describes how gamma-ray spectra are computed and the significance of response function parameters that define characteristics of particular detectors.

  5. Climate Change Response

    Energy Savers [EERE]

    the Interior Climate Change Response "From the Everglades to the Great Lakes to Alaska and everywhere in between, climate change is a leading threat to natural and cultural ...

  6. TECHNICAL STANDARDS PROGRAM RESPONSIBILITIES

    Broader source: Energy.gov [DOE]

    PurposeThis procedure describes the responsibilities of persons who are charged with implementing the DOE Technical Standards Program. 

  7. NNMCAB Responses from DOE

    Broader source: Energy.gov [DOE]

    Provides a listing of responses from the U.S. Department of Energy (DOE) to the recommendations provided from the Northern New Mexico Citizens’ Advisory Board (NNMCAB).

  8. Response Resources Demonstration

    Office of Environmental Management (EM)

    Interoperability of Demand Response Resources Demonstration in NY Final Technical Report Award Number: DE-FC26-08NT02869 Project Type: Regional Demonstration Principal ...

  9. CRA Comments & Responses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (51105) 11 Response to CRA Comments (92005) Enclosure 1 - Computer Code VerificationTesting (92005) Inventory and Performance Assessment Reports DOE - Performance...

  10. Adaptive Sampling for High Throughput Data Using Similarity Measures

    SciTech Connect (OSTI)

    Bulaevskaya, V.; Sales, A. P.

    2015-05-06

    The need for adaptive sampling arises in the context of high throughput data because the rates of data arrival are many orders of magnitude larger than the rates at which they can be analyzed. A very fast decision must therefore be made regarding the value of each incoming observation and its inclusion in the analysis. In this report we discuss one approach to adaptive sampling, based on the new data points similarity to the other data points being considered for inclusion. We present preliminary results for one real and one synthetic data set.

  11. Slidesurfaces with Adaptive New Definitions (SAND) for transient analysis

    SciTech Connect (OSTI)

    Whirley, R.G.; Engelmann, B.E.

    1992-06-02

    This paper describes some recent developments in adaptive contact algorithms for the transient analysis of penetration and material failure. A failure criterion is defined for volumes of potentially failing material on each side of a contact surface. As material within an element fails, the element is removed from the calculation and the contact surface is adaptively redefined to include the newly exposed outer material boundary. This contact algorithm admits arbitrary combinations of shell and solid elements to facilitate the representation of composite or honeycomb structures. The algorithms and their application are illustrated with several large-scale simulations using the explicit transient analysis code DYNA3D.

  12. U.S. Department of Energy Climate Change Adaptation Policy Statement |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Climate Change Adaptation Policy Statement U.S. Department of Energy Climate Change Adaptation Policy Statement U.S. Department of Energy statement that commits the agency to addressing the impacts climate change may have on operations and assets through adaptation planning. PDF icon doestatement_ccadaptationpolicy.pdf More Documents & Publications Climate Change Adaptation Policy Statement 2014 DOE Climate Change Adaptation Plan U.S. Department of Energy 2012

  13. Investigation of Yersinia pestis Laboratory Adaptation through a Combined Genomics and Proteomics Approach

    SciTech Connect (OSTI)

    Leiser, Owen P.; Merkley, Eric D.; Clowers, Brian H.; Kaiser, Brooke LD; Lin, Andy; Hutchison, Janine R.; Melville, Angela M.; Wagner, David M.; Keim, Paul S.; Foster, Jeff; Kreuzer, Helen W.

    2015-11-24

    The bacterial pathogen Yersinia pestis, the cause of plague in humans and animals, normally has a sylvatic lifestyle, cycling between fleas and mammals. In contrast, laboratory-grown Y. pestis experiences a more constant environment and conditions that it would not normally encounter. The transition from the natural environment to the laboratory results in a vastly different set of selective pressures, and represents what could be considered domestication. Understanding the kinds of adaptations Y. pestis undergoes as it becomes domesticated will contribute to understanding the basic biology of this important pathogen. In this study, we performed a Parallel Serial Passage Experiment (PSPE) to explore the mechanisms by which Y. pestis adapts to laboratory conditions, hypothesizing that cells would undergo significant changes in virulence and nutrient acquisition systems. Two wild strains were serially passaged in 12 independent populations each for ~750 generations, after which each population was analyzed using whole-genome sequencing. We observed considerable parallel evolution in the endpoint populations, detecting multiple independent mutations in ail, pepA, and zwf, suggesting that specific selective pressures are shaping evolutionary responses. Complementary LC-MS-based proteomic data provide physiological context to the observed mutations, and reveal regulatory changes not necessarily associated with specific mutations, including changes in amino acid metabolism, envelope biogenesis, iron storage and acquisition, and a type VI secretion system. Proteomic data support hypotheses generated by genomic data in addition to suggesting future mechanistic studies, indicating that future whole-genome sequencing studies be designed to leverage proteomics as a critical complement.

  14. Adaptive Classification of Landscape Process and Function: An Integration of Geoinformatics and Self-Organizing Maps

    SciTech Connect (OSTI)

    Coleman, Andre M.

    2009-07-17

    The advanced geospatial information extraction and analysis capabilities of a Geographic Information System (GISs) and Artificial Neural Networks (ANNs), particularly Self-Organizing Maps (SOMs), provide a topology-preserving means for reducing and understanding complex data relationships in the landscape. The Adaptive Landscape Classification Procedure (ALCP) is presented as an adaptive and evolutionary capability where varying types of data can be assimilated to address different management needs such as hydrologic response, erosion potential, habitat structure, instrumentation placement, and various forecast or what-if scenarios. This paper defines how the evaluation and analysis of spatial and/or temporal patterns in the landscape can provide insight into complex ecological, hydrological, climatic, and other natural and anthropogenic-influenced processes. Establishing relationships among high-dimensional datasets through neurocomputing based pattern recognition methods can help 1) resolve large volumes of data into a structured and meaningful form; 2) provide an approach for inferring landscape processes in areas that have limited data available but exhibit similar landscape characteristics; and 3) discover the value of individual variables or groups of variables that contribute to specific processes in the landscape. Classification of hydrologic patterns in the landscape is demonstrated.

  15. Cold adaptation of the mononuclear molybdoenzyme periplasmic nitrate reductase from the Antarctic bacterium Shewanella gelidimarina

    SciTech Connect (OSTI)

    Simpson, Philippa J.L.; Codd, Rachel; School of Medical Sciences and Bosch Institute, University of New South Wales, New South Wales 2006

    2011-11-04

    Highlights: Black-Right-Pointing-Pointer Cold-adapted phenotype of NapA from the Antarctic bacterium Shewanella gelidimarina. Black-Right-Pointing-Pointer Protein homology model of NapA from S. gelidimarina and mesophilic homologue. Black-Right-Pointing-Pointer Six amino acid residues identified as lead candidates governing NapA cold adaptation. Black-Right-Pointing-Pointer Molecular-level understanding of designing cool-temperature in situ oxyanion sensors. -- Abstract: The reduction of nitrate to nitrite is catalysed in bacteria by periplasmic nitrate reductase (Nap) which describes a system of variable protein subunits encoded by the nap operon. Nitrate reduction occurs in the NapA subunit, which contains a bis-molybdopterin guanine dinucleotide (Mo-MGD) cofactor and one [4Fe-4S] iron-sulfur cluster. The activity of periplasmic nitrate reductase (Nap) isolated as native protein from the cold-adapted (psychrophilic) Antarctic bacterium Shewanella gelidimarina (Nap{sub Sgel}) and middle-temperature adapted (mesophilic) Shewanella putrefaciens (Nap{sub Sput}) was examined at varied temperature. Irreversible deactivation of Nap{sub Sgel} and Nap{sub Sput} occurred at 54.5 and 65 Degree-Sign C, respectively. When Nap{sub Sgel} was preincubated at 21-70 Degree-Sign C for 30 min, the room-temperature nitrate reductase activity was maximal and invariant between 21 and 54 Degree-Sign C, which suggested that Nap{sub Sgel} was poised for optimal catalysis at modest temperatures and, unlike Nap{sub Sput}, did not benefit from thermally-induced refolding. At 20 Degree-Sign C, Nap{sub Sgel} reduced selenate at 16% of the rate of nitrate reduction. Nap{sub Sput} did not reduce selenate. Sequence alignment showed 46 amino acid residue substitutions in Nap{sub Sgel} that were conserved in NapA from mesophilic Shewanella, Rhodobacter and Escherichia species and could be associated with the Nap{sub Sgel} cold-adapted phenotype. Protein homology modeling of Nap{sub Sgel} using a mesophilic template with 66% amino acid identity showed the majority of substitutions occurred at the protein surface distal to the Mo-MGD cofactor. Two mesophilic {r_reversible} psychrophilic substitutions (Asn {r_reversible} His, Val {r_reversible} Trp) occurred in a region close to the surface of the NapA substrate funnel resulting in potential interdomain {pi}-{pi} and/or cation-{pi} interactions. Three mesophilic {r_reversible} psychrophilic substitutions occurred within 4.5 A of the Mo-MGD cofactor (Phe {r_reversible} Met, Ala {r_reversible} Ser, Ser {r_reversible} Thr) resulting in local regions that varied in hydrophobicity and hydrogen bonding networks. These results contribute to the understanding of thermal protein adaptation in a redox-active mononuclear molybdenum enzyme and have implications in optimizing the design of low-temperature environmental biosensors.

  16. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Twitter Google + Vimeo GovDelivery SlideShare Laser-induced incandescence (LII) HomeClimate & Earth SystemsClimate Measurement & ModelingLaser-induced incandescence (LII) ...

  17. Eastern Frequency Response Study

    SciTech Connect (OSTI)

    Miller, N.W.; Shao, M.; Pajic, S.; D'Aquila, R.

    2013-05-01

    This study was specifically designed to investigate the frequency response of the Eastern Interconnection that results from large loss-of-generation events of the type targeted by the North American Electric Reliability Corp. Standard BAL-003 Frequency Response and Frequency Bias Setting (NERC 2012a), under possible future system conditions with high levels of wind generation.

  18. Transition from Irradiation-Induced Amorphization to Crystallization in

    Office of Scientific and Technical Information (OSTI)

    Nanocrystalline Silicon Carbide (Journal Article) | SciTech Connect Journal Article: Transition from Irradiation-Induced Amorphization to Crystallization in Nanocrystalline Silicon Carbide Citation Details In-Document Search Title: Transition from Irradiation-Induced Amorphization to Crystallization in Nanocrystalline Silicon Carbide Response to irradiation of nanocrystalline 3C-SiC is studied using 2 MeV Au+ ions near the critical temperature for amorphization and is compared to the

  19. Analyte sensing mediated by adapter/carrier molecules

    DOE Patents [OSTI]

    Bayley, Hagan; Braha, Orit; Gu, LiQun

    2002-07-30

    This invention relates to an improved method and system for sensing of one or more analytes. A host molecule, which serves as an adapter/carrier, is used to facilitate interaction between the analyte and the sensor element. A detectable signal is produced reflecting the identity and concentration of analyte present.

  20. An adaptive wavelet stochastic collocation method for irregular solutions

    Office of Scientific and Technical Information (OSTI)

    of stochastic partial differential equations (Technical Report) | SciTech Connect wavelet stochastic collocation method for irregular solutions of stochastic partial differential equations Citation Details In-Document Search Title: An adaptive wavelet stochastic collocation method for irregular solutions of stochastic partial differential equations Accurate predictive simulations of complex real world applications require numerical approximations to first, oppose the curse of dimensionality

  1. Static & Dynamic Response of 2D Solids

    Energy Science and Technology Software Center (OSTI)

    1996-07-15

    NIKE2D is an implicit finite-element code for analyzing the finite deformation, static and dynamic response of two-dimensional, axisymmetric, plane strain, and plane stress solids. The code is fully vectorized and available on several computing platforms. A number of material models are incorporated to simulate a wide range of material behavior including elasto-placicity, anisotropy, creep, thermal effects, and rate dependence. Slideline algorithms model gaps and sliding along material interfaces, including interface friction, penetration and single surfacemore » contact. Interactive-graphics and rezoning is included for analyses with large mesh distortions. In addition to quasi-Newton and arc-length procedures, adaptive algorithms can be defined to solve the implicit equations using the solution language ISLAND. Each of these capabilities and more make NIKE2D a robust analysis tool.« less

  2. Static & Dynamic Response of 2D Solids

    Energy Science and Technology Software Center (OSTI)

    1996-07-15

    NIKE2D is an implicit finite-element code for analyzing the finite deformation, static and dynamic response of two-dimensional, axisymmetric, plane strain, and plane stress solids. The code is fully vectorized and available on several computing platforms. A number of material models are incorporated to simulate a wide range of material behavior including elasto-placicity, anisotropy, creep, thermal effects, and rate dependence. Slideline algorithms model gaps and sliding along material interfaces, including interface friction, penetration and single surfacemorecontact. Interactive-graphics and rezoning is included for analyses with large mesh distortions. In addition to quasi-Newton and arc-length procedures, adaptive algorithms can be defined to solve the implicit equations using the solution language ISLAND. Each of these capabilities and more make NIKE2D a robust analysis tool.less

  3. A wavelet-MRA-based adaptive semi-Lagrangian method for the relativistic Vlasov-Maxwell system

    SciTech Connect (OSTI)

    Besse, Nicolas Latu, Guillaume Ghizzo, Alain Sonnendruecker, Eric Bertrand, Pierre

    2008-08-10

    In this paper we present a new method for the numerical solution of the relativistic Vlasov-Maxwell system on a phase-space grid using an adaptive semi-Lagrangian method. The adaptivity is performed through a wavelet multiresolution analysis, which gives a powerful and natural refinement criterion based on the local measurement of the approximation error and regularity of the distribution function. Therefore, the multiscale expansion of the distribution function allows to get a sparse representation of the data and thus save memory space and CPU time. We apply this numerical scheme to reduced Vlasov-Maxwell systems arising in laser-plasma physics. Interaction of relativistically strong laser pulses with overdense plasma slabs is investigated. These Vlasov simulations revealed a rich variety of phenomena associated with the fast particle dynamics induced by electromagnetic waves as electron trapping, particle acceleration, and electron plasma wavebreaking. However, the wavelet based adaptive method that we developed here, does not yield significant improvements compared to Vlasov solvers on a uniform mesh due to the substantial overhead that the method introduces. Nonetheless they might be a first step towards more efficient adaptive solvers based on different ideas for the grid refinement or on a more efficient implementation. Here the Vlasov simulations are performed in a two-dimensional phase-space where the development of thin filaments, strongly amplified by relativistic effects requires an important increase of the total number of points of the phase-space grid as they get finer as time goes on. The adaptive method could be more useful in cases where these thin filaments that need to be resolved are a very small fraction of the hyper-volume, which arises in higher dimensions because of the surface-to-volume scaling and the essentially one-dimensional structure of the filaments. Moreover, the main way to improve the efficiency of the adaptive method is to increase the local character in phase-space of the numerical scheme, by considering multiscale reconstruction with more compact support and by replacing the semi-Lagrangian method with more local - in space - numerical scheme as compact finite difference schemes, discontinuous-Galerkin method or finite element residual schemes which are well suited for parallel domain decomposition techniques.

  4. Frequency Response Tool

    Energy Science and Technology Software Center (OSTI)

    2014-03-13

    According to the North American Electric Reliability Corporation (NERC) definition: “Frequency response is a measure of an Interconnection’s ability to stabilize frequency immediately following the sudden loss of generation or load, and is a critical component of the reliable operation of the Bulk-Power System, particularly during disturbances and recoveries. Failure to maintain frequency can disrupt the operation of equipment and initiate disconnection of power plant equipment to prevent it from being damaged, which could leadmore » to wide-spread blackouts.” Frequency Response Tool automates the power system frequency response analysis process. The tool performs initial estimation of the system frequency parameters (initial frequency, minimum frequency, settling point). User can visually inspect and adjust these parameters. The tool also calculates the frequency response performance metrics of the system, archives the historic events and baselines the system performance. Frequency response performance characteristics of the system are calculated using phasor measurement unit (PMU) information. Methodology of the frequency response performance assessment implemented in the tool complies with the NERC Frequency response standard.« less

  5. Frequency Response Analysis Tool

    SciTech Connect (OSTI)

    Etingov, Pavel V.; Kosterev, Dmitry; Dai, T.

    2014-12-31

    Frequency response has received a lot of attention in recent years at the national level, which culminated in the development and approval of North American Electricity Reliability Corporation (NERC) BAL-003-1 Frequency Response and Frequency Bias Setting Reliability Standard. This report is prepared to describe the details of the work conducted by Pacific Northwest National Laboratory (PNNL) in collaboration with the Bonneville Power Administration and Western Electricity Coordinating Council (WECC) Joint Synchronized Information Subcommittee (JSIS) to develop a frequency response analysis tool (FRAT). The document provides the details on the methodology and main features of the FRAT. The tool manages the database of under-frequency events and calculates the frequency response baseline. Frequency response calculations are consistent with frequency response measure (FRM) in NERC BAL-003-1 for an interconnection and balancing authority. The FRAT can use both phasor measurement unit (PMU) data, where available, and supervisory control and data acquisition (SCADA) data. The tool is also capable of automatically generating NERC Frequency Response Survey (FRS) forms required by BAL-003-1 Standard.

  6. Demand Response Analysis Tool

    Energy Science and Technology Software Center (OSTI)

    2012-03-01

    Demand Response Analysis Tool is a software developed at the Lawrence Berkeley National Laboratory. It is initially funded by Southern California Edison. Our goal in developing this tool is to provide an online, useable, with standardized methods, an analysis tool to evaluate demand and demand response performance of commercial and industrial facilities. The tool provides load variability and weather sensitivity analysis capabilities as well as development of various types of baselines. It can be usedmore » by researchers, real estate management firms, utilities, or any individuals who are interested in analyzing their demand and demand response capabilities.« less

  7. Demand Response Analysis Tool

    SciTech Connect (OSTI)

    2012-03-01

    Demand Response Analysis Tool is a software developed at the Lawrence Berkeley National Laboratory. It is initially funded by Southern California Edison. Our goal in developing this tool is to provide an online, useable, with standardized methods, an analysis tool to evaluate demand and demand response performance of commercial and industrial facilities. The tool provides load variability and weather sensitivity analysis capabilities as well as development of various types of baselines. It can be used by researchers, real estate management firms, utilities, or any individuals who are interested in analyzing their demand and demand response capabilities.

  8. COMMENTS AND RESPONSES

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Comments and Responses to the Tentative Agreement Regarding The Fast Flux Test Facility (Agreement Major Milestone Series M-81-00) August 1999 2 COMMENTS AND RESPONSES TO THE TENTATIVE AGREEMENT REGARDING THE FAST FLUX TEST FACILITY COMMENTS AND RESPONSES 1. Introduction In January 1997 the U.S. Department of Energy (DOE) changed the status of the Fast Flux Test Facility (FFTF) from deactivation to standby pending a decision, to be made by December 1998 on whether or not the facility will be

  9. DAiSES: Dynamic Adaptivity in Support of Extreme Scale Department of Energy Project No. ER25622 Prime Contract No. DE-FG02-04ER25622 Final Report for September 15, 2004-September 14, 2008

    SciTech Connect (OSTI)

    PI: Patricia J. Teller, Ph.D.

    2009-05-05

    The DAiSES project [Te04] was focused on enabling conventional operating systems, in particular, those running on extreme scale systems, to dynamically customize system resource management in order to offer applications the best possible environment in which to execute. Such dynamic adaptation allows operating systems to modify the execution environment in response to changes in workload behavior and system state. The main challenges of this project included determination of what operating system (OS) algorithms, policies, and parameters should be adapted, when to adapt them, and how to adapt them. We addressed these challenges by using a combination of static analysis and runtime monitoring and adaptation to identify a priori profitable targets of adaptation and effective heuristics that can be used to dynamically trigger adaptation. Dynamic monitoring and adaptation of the OS was provided by either kernel modifications or the use of KernInst and Kperfmon [Wm04]. Since Linux, an open source OS, was our target OS, patches submitted by kernel developers and researchers often facilitated kernel modifications. KernInst operates on unmodified commodity operating systems, i.e., Solaris and Linux; it is fine-grained, thus, there were few constraints on how the underlying OS can be modified. Dynamically adaptive functionality of operating systems, both in terms of policies and parameters, is intended to deliver the maximum attainable performance of a computational environment and meet, as best as possible, the needs of high-performance applications running on extreme scale systems, while meeting system constraints. DAiSES research endeavored to reach this goal by developing methodologies for dynamic adaptation of OS parameters and policies to manage stateful and stateless resources [Te06] and pursuing the following two objectives: (1) Development of mechanisms to dynamically sense, analyze, and adjust common performance metrics, fluctuating workload situations, and overall system environment conditions. (2) Demonstration, via Linux prototypes and experiments, of dynamic self-tuning and self-provisioning in HPC environments. From a high level, the DAiSES methodology, depicted in Figure 1, includes characterization of application resource usage patterns, identification of candidate (profitable) adaptation targets, determination of feasible adaptation ranges, definition of heuristics to trigger adaptation, design and implementation of OS monitoring, triggering, and adaptation code, and quantification of performance gains.

  10. Intelligent Software Agents: Sensor Integration and Response

    SciTech Connect (OSTI)

    Kulesz, James J; Lee, Ronald W

    2013-01-01

    Abstract In a post Macondo world the buzzwords are Integrity Management and Incident Response Management. The twin processes are not new but the opportunity to link the two is novel. Intelligent software agents can be used with sensor networks in distributed and centralized computing systems to enhance real-time monitoring of system integrity as well as manage the follow-on incident response to changing, and potentially hazardous, environmental conditions. The software components are embedded at the sensor network nodes in surveillance systems used for monitoring unusual events. When an event occurs, the software agents establish a new concept of operation at the sensing node, post the event status to a blackboard for software agents at other nodes to see , and then react quickly and efficiently to monitor the scale of the event. The technology addresses a current challenge in sensor networks that prevents a rapid and efficient response when a sensor measurement indicates that an event has occurred. By using intelligent software agents - which can be stationary or mobile, interact socially, and adapt to changing situations - the technology offers features that are particularly important when systems need to adapt to active circumstances. For example, when a release is detected, the local software agent collaborates with other agents at the node to exercise the appropriate operation, such as: targeted detection, increased detection frequency, decreased detection frequency for other non-alarming sensors, and determination of environmental conditions so that adjacent nodes can be informed that an event is occurring and when it will arrive. The software agents at the nodes can also post the data in a targeted manner, so that agents at other nodes and the command center can exercise appropriate operations to recalibrate the overall sensor network and associated intelligence systems. The paper describes the concepts and provides examples of real-world implementations including the Threat Detection and Analysis System (TDAS) at the International Port of Memphis and the Biological Warning and Incident Characterization System (BWIC) Environmental Monitoring (EM) Component. Technologies developed for these 24/7 operational systems have applications for improved real-time system integrity awareness as well as provide incident response (as needed) for production and field applications.

  11. Your Records Management Responsibilities

    Broader source: Energy.gov [DOE]

    As a DOE federal or contractor employee, you will create or receive official records, and you are responsible for managing those records as part of accomplishing the Department's mission and...

  12. NETL Emergency Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NETL Emergency Response For Employees NETL Emergency Response/Continuity of Operations for Employees This website is for postings of special instructions for personnel employed at NETL and for our surrounding communities. Announcements posted to this site could include: Broadcast instructions to employees and essential personnel during continuity of operations execution or emergencies Copies of informational press releases issued to the public during emergencies These phone numbers are for the

  13. Your Records Management Responsibilities

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Your Records Management Responsibilities Table of Contents INTRODUCTION RECORDS MANAGEMENT IN THE FEDERAL GOVERNMENT RECORDS MANAGEMENT IN THE DEPARTMENT OF ENERGY IMPORTANCE OF RECORDS MANAGEMENT YOUR RECORDS MANAGEMENT RESPONSIBILITIES RECORDS MANAGEMENT LIFE CYCLE ELECTRONIC RECORDS & RECORDKEEPING LAW, REGULATION, AND POLICY ASSISTANCE RECORDS MANAGEMENT TERMS 2 INTRODUCTION If you are a government employee or contractor working for a federal agency, records management is part of your

  14. Demand Response Dispatch Tool

    SciTech Connect (OSTI)

    2012-08-31

    The Demand Response (DR) Dispatch Tool uses price profiles to dispatch demand response resources and create load modifying profiles. These annual profiles are used as inputs to production cost models and regional planning tools (e.g., PROMOD). The tool has been effectively implemented in transmission planning studies conducted by the Western Electricity Coordinating Council via its Transmission Expansion Planning and Policy Committee. The DR Dispatch Tool can properly model the dispatch of DR resources for both reliability and economic conditions.

  15. CRA Comments & Responses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CRA Comments & Responses Home CRA - 2004 Final Recertification Decision CRA Comments & Responses CCA - 1996 CRA CARDs & TSDs CCA CARDs & TSDs Regulatory Tools Completeness Determination-Related Correspondence All EPA & DOE correspondence related to the determination of completeness and the CRA are provided below. Letter Transmitting EPA's CRA Completeness Determination (9/29/05) Notification of Completeness Determination EPA Letter # Subject 1 CRA Comments (5/20/04)

  16. Questions and Responses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Responses: Question 1: Would the Government consider accepting either CMMI-DEV or CMMI-SVC Level 3 or higher since there is majority of process areas are shared between the two constellations and repeatability of processes will not be reduced since both will require Level 3 or higher? Government Response: The Government has determined that CMMI-SVC is a relatively new methodology and is in agreement that there is a considerable overlap of process areas shared between the two constellations. The

  17. Advanced numerical methods in mesh generation and mesh adaptation

    SciTech Connect (OSTI)

    Lipnikov, Konstantine; Danilov, A; Vassilevski, Y; Agonzal, A

    2010-01-01

    Numerical solution of partial differential equations requires appropriate meshes, efficient solvers and robust and reliable error estimates. Generation of high-quality meshes for complex engineering models is a non-trivial task. This task is made more difficult when the mesh has to be adapted to a problem solution. This article is focused on a synergistic approach to the mesh generation and mesh adaptation, where best properties of various mesh generation methods are combined to build efficiently simplicial meshes. First, the advancing front technique (AFT) is combined with the incremental Delaunay triangulation (DT) to build an initial mesh. Second, the metric-based mesh adaptation (MBA) method is employed to improve quality of the generated mesh and/or to adapt it to a problem solution. We demonstrate with numerical experiments that combination of all three methods is required for robust meshing of complex engineering models. The key to successful mesh generation is the high-quality of the triangles in the initial front. We use a black-box technique to improve surface meshes exported from an unattainable CAD system. The initial surface mesh is refined into a shape-regular triangulation which approximates the boundary with the same accuracy as the CAD mesh. The DT method adds robustness to the AFT. The resulting mesh is topologically correct but may contain a few slivers. The MBA uses seven local operations to modify the mesh topology. It improves significantly the mesh quality. The MBA method is also used to adapt the mesh to a problem solution to minimize computational resources required for solving the problem. The MBA has a solid theoretical background. In the first two experiments, we consider the convection-diffusion and elasticity problems. We demonstrate the optimal reduction rate of the discretization error on a sequence of adaptive strongly anisotropic meshes. The key element of the MBA method is construction of a tensor metric from hierarchical edge-based error estimates. We conclude that the quasi-optimal mesh must be quasi-uniform in this metric. All numerical experiments are based on the publicly available Ani3D package, the collection of advanced numerical instruments.

  18. Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hu, Bo; Yang, Yi -Ming; Beck, David A. C.; Wang, Qian -Wen; Chen, Wen -Jing; Yang, Jing; Lidstrom, Mary E.; Yang, Song

    2016-04-11

    In this study, the toxicity of alcohols is one of the major roadblocks of biological fermentation for biofuels production. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, has been engineered to generate 1-butanol from cheap carbon feedstocks through a synthetic metabolic pathway. However, M. extorquens AM1 is vulnerable to solvent stress, which impedes further development for 1-butanol production. Only a few studies have reported the general stress response of M. extorquens AM1 to solvent stress. Therefore, it is highly desirable to obtain a strain with ameliorated 1-butanol tolerance and elucidate the molecular mechanism of 1-butnaol tolerance in M. extorquens AM1 formore » future strain improvement. In this work, adaptive laboratory evolution was used as a tool to isolate mutants with 1-butanol tolerance up to 0.5 %. The evolved strains, BHBT3 and BHBT5, demonstrated increased growth rates and higher survival rates with the existence of 1-butanol. Whole genome sequencing revealed a SNP mutation at kefB in BHBT5, which was confirmed to be responsible for increasing 1-butanol tolerance through an allelic exchange experiment. Global metabolomic analysis further discovered that the pools of multiple key metabolites, including fatty acids, amino acids, and disaccharides, were increased in BHBT5 in response to 1-butanol stress. Additionally, the carotenoid synthesis pathway was significantly down-regulated in BHBT5. In conclusion, we successfully screened mutants resistant to 1-butanol and provided insights into the molecular mechanism of 1-butanol tolerance in M. extorquens AM1. This research will be useful for uncovering the mechanism of cellular response of M. extorquens AM1 to solvent stress, and will provide the genetic blueprint for the rational design of a strain of M. extorquens AM1 with increased 1-butanol tolerance in the future.« less

  19. Caffeic acid phenethyl ester inhibits 3-MC-induced CYP1A1 expression through induction of hypoxia-inducible factor-1α

    SciTech Connect (OSTI)

    Kim, Hyung Gyun; Han, Eun Hee; Im, Ji Hye; Lee, Eun Ji; Jin, Sun Woo; Jeong, Hye Gwang

    2015-09-25

    Caffeic acid phenethyl ester (CAPE), a natural component of propolis, is reported to have anticarcinogenic properties, although its precise chemopreventive mechanism remains unclear. In this study, we examined the effects of CAPE on 3-methylcholanthrene (3-MC)-induced CYP1A1 expression and activities. CAPE reduced the formation of the benzo[a]pyrene-DNA adduct. Moreover, CAPE inhibited 3-MC-induced CYP1A1 activity, mRNA expression, protein level, and promoter activity. CAPE treatment also decreased 3-MC-inducible xenobiotic-response element (XRE)-linked luciferase, aryl hydrocarbons receptor (AhR) transactivation and nuclear localization. CAPE induced hypoxia inducible factor-1α (HIF-1α) protein level and HIF-1α responsible element (HRE) transcriptional activity. CAPE-mediated HIF-1α reduced 3-MC-inducible CYP1A1 protein expression. Taken together, CAPE decreases 3-MC-mediated CYP1A1 expression, and this inhibitory response is associated with inhibition of AhR and HIF-1α induction. - Highlights: • CAPE reduced the formation of the benzo[a]pyrene-DNA adduct. • CAPE inhibited 3-MC-induced CYP1A1 expression. • CAPE induced HIF-1α induction. • CAPE-mediated HIF-1α reduced 3-MC-inducible CYP1A1 expression.

  20. Adaptive reconnection-based arbitrary Lagrangian Eulerian method

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bo, Wurigen; Shashkov, Mikhail

    2015-07-21

    We present a new adaptive Arbitrary Lagrangian Eulerian (ALE) method. This method is based on the reconnection-based ALE (ReALE) methodology of Refs. [35], [34] and [6]. The main elements in a standard ReALE method are: an explicit Lagrangian phase on an arbitrary polygonal (in 2D) mesh in which the solution and positions of grid nodes are updated; a rezoning phase in which a new grid is defined by changing the connectivity (using Voronoi tessellation) but not the number of cells; and a remapping phase in which the Lagrangian solution is transferred onto the new grid. Furthermore, in the standard ReALEmore » method, the rezoned mesh is smoothed by using one or several steps toward centroidal Voronoi tessellation, but it is not adapted to the solution in any way.« less

  1. Adaptive pitch control for variable speed wind turbines

    DOE Patents [OSTI]

    Johnson, Kathryn E.; Fingersh, Lee Jay

    2012-05-08

    An adaptive method for adjusting blade pitch angle, and controllers implementing such a method, for achieving higher power coefficients. Average power coefficients are determined for first and second periods of operation for the wind turbine. When the average power coefficient for the second time period is larger than for the first, a pitch increment, which may be generated based on the power coefficients, is added (or the sign is retained) to the nominal pitch angle value for the wind turbine. When the average power coefficient for the second time period is less than for the first, the pitch increment is subtracted (or the sign is changed). A control signal is generated based on the adapted pitch angle value and sent to blade pitch actuators that act to change the pitch angle of the wind turbine to the new or modified pitch angle setting, and this process is iteratively performed.

  2. Modeling scramjet combustor flowfields with a grid adaptation scheme

    SciTech Connect (OSTI)

    Ramakrishnan, R.; Singh, D.J. )

    1994-05-01

    The accurate description of flow features associated with the normal injection of fuel into supersonic primary flows is essential in the design of efficient engines for hypervelocity aerospace vehicles. The flow features in such injections are complex with multiple interactions between shocks and between shocks and boundary layers. Numerical studies of perpendicular sonic N2 injection and mixing in a Mach 3.8 scramjet combustor environment are discussed. A dynamic grid adaptation procedure based on the equilibration of spring-mass systems is employed to enhance the description of the complicated flow features. Numerical results are compared with experimental measurements and indicate that the adaptation procedure enhances the capability of the modeling procedure to describe the flow features associated with scramjet combustor components. 14 refs.

  3. System and Method for Vehicle Energy Estimation, Adaptive Control and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Routing - Energy Innovation Portal Vehicles and Fuels Vehicles and Fuels Energy Analysis Energy Analysis Find More Like This Return to Search System and Method for Vehicle Energy Estimation, Adaptive Control and Routing National Renewable Energy Laboratory Contact NREL About This Technology Technology Marketing Summary In the fields of vehicle telematics and navigation, there are algorithms for recommending potential travel routes from a given origin to a desired destination. Such potential

  4. Adaptive pitch control for variable speed wind turbines - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal 174,136 Site Map Printable Version Share this resource About Search Categories (15) Advanced Materials Biomass and Biofuels Building Energy Efficiency Electricity Transmission Energy Analysis Energy Storage Geothermal Hydrogen and Fuel Cell Hydropower, Wave and Tidal Industrial Technologies Solar Photovoltaic Solar Thermal Startup America Vehicles and Fuels Wind Energy Partners (27) Visual Patent Search Success Stories Find More Like This Return to Search Adaptive pitch control for

  5. Framework for Adaptable Operating and Runtime Systems: Final Project Report

    SciTech Connect (OSTI)

    Patrick G. Bridges

    2012-02-01

    In this grant, we examined a wide range of techniques for constructing high-performance con#12;gurable system software for HPC systems and its application to DOE-relevant problems. Overall, research and development on this project focused in three specifc areas: (1) software frameworks for constructing and deploying con#12;gurable system software, (2) applcation of these frameworks to HPC-oriented adaptable networking software, (3) performance analysis of HPC system software to understand opportunities for performance optimization.

  6. Adaption of Machine Fluid Analysis for Manufacturing - Final Report

    SciTech Connect (OSTI)

    Pardini, Allan F.

    2005-08-16

    Pacific Northwest National Laboratory (PNNL: Operated by Battelle Memorial Institute for the Department of Energy) is working with the Department of Energy (DOE) to develop technology for the US mining industry. Filtration and lubricant suppliers to the pulp and paper industry had noted the recent accomplishments by PNNL and its industrial partners in the DOE OIT Mining Industry of the Future Program, and asked for assistance in adapting this DOE-funded technology to the pulp and paper industry.

  7. Distributed database kriging for adaptive sampling (D²KAS)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Roehm, Dominic; Pavel, Robert S.; Barros, Kipton; Rouet-Leduc, Bertrand; McPherson, Allen L.; Germann, Timothy C.; Junghans, Christoph

    2015-03-18

    We present an adaptive sampling method supplemented by a distributed database and a prediction method for multiscale simulations using the Heterogeneous Multiscale Method. A finite-volume scheme integrates the macro-scale conservation laws for elastodynamics, which are closed by momentum and energy fluxes evaluated at the micro-scale. In the original approach, molecular dynamics (MD) simulations are launched for every macro-scale volume element. Our adaptive sampling scheme replaces a large fraction of costly micro-scale MD simulations with fast table lookup and prediction. The cloud database Redis provides the plain table lookup, and with locality aware hashing we gather input data for our predictionmore » scheme. For the latter we use kriging, which estimates an unknown value and its uncertainty (error) at a specific location in parameter space by using weighted averages of the neighboring points. We find that our adaptive scheme significantly improves simulation performance by a factor of 2.5 to 25, while retaining high accuracy for various choices of the algorithm parameters.« less

  8. Advances in Patch-Based Adaptive Mesh Refinement Scalability

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gunney, Brian T.N.; Anderson, Robert W.

    2015-12-18

    Patch-based structured adaptive mesh refinement (SAMR) is widely used for high-resolution simu- lations. Combined with modern supercomputers, it could provide simulations of unprecedented size and resolution. A persistent challenge for this com- bination has been managing dynamically adaptive meshes on more and more MPI tasks. The dis- tributed mesh management scheme in SAMRAI has made some progress SAMR scalability, but early al- gorithms still had trouble scaling past the regime of 105 MPI tasks. This work provides two critical SAMR regridding algorithms, which are integrated into that scheme to ensure efficiency of the whole. The clustering algorithm is an extensionmore » of the tile- clustering approach, making it more flexible and efficient in both clustering and parallelism. The partitioner is a new algorithm designed to prevent the network congestion experienced by its prede- cessor. We evaluated performance using weak- and strong-scaling benchmarks designed to be difficult for dynamic adaptivity. Results show good scaling on up to 1.5M cores and 2M MPI tasks. Detailed timing diagnostics suggest scaling would continue well past that.« less

  9. Data Assimilation in the ADAPT Photospheric Flux Transport Model

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hickmann, Kyle S.; Godinez, Humberto C.; Henney, Carl J.; Arge, C. Nick

    2015-03-17

    Global maps of the solar photospheric magnetic flux are fundamental drivers for simulations of the corona and solar wind and therefore are important predictors of geoeffective events. However, observations of the solar photosphere are only made intermittently over approximately half of the solar surface. The Air Force Data Assimilative Photospheric Flux Transport (ADAPT) model uses localized ensemble Kalman filtering techniques to adjust a set of photospheric simulations to agree with the available observations. At the same time, this information is propagated to areas of the simulation that have not been observed. ADAPT implements a local ensemble transform Kalman filter (LETKF)more » to accomplish data assimilation, allowing the covariance structure of the flux-transport model to influence assimilation of photosphere observations while eliminating spurious correlations between ensemble members arising from a limited ensemble size. We give a detailed account of the implementation of the LETKF into ADAPT. Advantages of the LETKF scheme over previously implemented assimilation methods are highlighted.« less

  10. Advances in Patch-Based Adaptive Mesh Refinement Scalability

    SciTech Connect (OSTI)

    Gunney, Brian T.N.; Anderson, Robert W.

    2015-12-18

    Patch-based structured adaptive mesh refinement (SAMR) is widely used for high-resolution simu- lations. Combined with modern supercomputers, it could provide simulations of unprecedented size and resolution. A persistent challenge for this com- bination has been managing dynamically adaptive meshes on more and more MPI tasks. The dis- tributed mesh management scheme in SAMRAI has made some progress SAMR scalability, but early al- gorithms still had trouble scaling past the regime of 105 MPI tasks. This work provides two critical SAMR regridding algorithms, which are integrated into that scheme to ensure efficiency of the whole. The clustering algorithm is an extension of the tile- clustering approach, making it more flexible and efficient in both clustering and parallelism. The partitioner is a new algorithm designed to prevent the network congestion experienced by its prede- cessor. We evaluated performance using weak- and strong-scaling benchmarks designed to be difficult for dynamic adaptivity. Results show good scaling on up to 1.5M cores and 2M MPI tasks. Detailed timing diagnostics suggest scaling would continue well past that.

  11. Coherent Image Layout using an Adaptive Visual Vocabulary

    SciTech Connect (OSTI)

    Dillard, Scott E.; Henry, Michael J.; Bohn, Shawn J.; Gosink, Luke J.

    2013-03-06

    When querying a huge image database containing millions of images, the result of the query may still contain many thousands of images that need to be presented to the user. We consider the problem of arranging such a large set of images into a visually coherent layout, one that places similar images next to each other. Image similarity is determined using a bag-of-features model, and the layout is constructed from a hierarchical clustering of the image set by mapping an in-order traversal of the hierarchy tree into a space-filling curve. This layout method provides strong locality guarantees so we are able to quantitatively evaluate performance using standard image retrieval benchmarks. Performance of the bag-of-features method is best when the vocabulary is learned on the image set being clustered. Because learning a large, discriminative vocabulary is a computationally demanding task, we present a novel method for efficiently adapting a generic visual vocabulary to a particular dataset. We evaluate our clustering and vocabulary adaptation methods on a variety of image datasets and show that adapting a generic vocabulary to a particular set of images improves performance on both hierarchical clustering and image retrieval tasks.

  12. Adapting classical Systems Engineering to Department of Energy (DOE) needs

    SciTech Connect (OSTI)

    1996-07-01

    Rather than discuss Systems Engineering (SE) as applied by aerospace contractors to military programs, this document provides an adapted model well suited for use by DOE and represents 18 months of applying SE principles to the challenges faced by INEL. The real-life examples are drawn from INEL`s ongoing effort to integrate activities across the entire spectrum of within its Environmental Management program. Since the traditional SE process, with its initial focus on requirements identification and analysis, must be modified to provide tangible results in the short term, the adapted SE model starts with the external driver of ``reducing costs without increasing risks`` and performs an initial integration effort to identify high-potential, cost-saving opportunities. Elements from traditional alternatives development and analysis stages are used; then the adapted model cycles back to include more traditional requirements analysis activities. These cycles continue in an iterative manner, adding rigor and detail at each successive iteration, throughout the life-cycle of a program or project. Detailed lessons learned are included.

  13. Time series analysis of Adaptive Optics wave-front sensor telemetry...

    Office of Scientific and Technical Information (OSTI)

    Time series analysis of Adaptive Optics wave-front sensor telemetry data Citation Details In-Document Search Title: Time series analysis of Adaptive Optics wave-front sensor ...

  14. An Adaptive Multi-Grid Chemistry (AMC) Model for Efficient Simulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    An Adaptive Multi-Grid Chemistry (AMC) Model for Efficient Simulation of HCCI and DI Engine Combustion An Adaptive Multi-Grid Chemistry (AMC) Model for Efficient Simulation of HCCI ...

  15. High-Efficiency, Wide-Band Three-Phase Rectifiers and Adaptive...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Efficiency, Wide-Band Three-Phase Rectifiers and Adaptive Rectifier Management High-Efficiency, Wide-Band Three-Phase Rectifiers and Adaptive Rectifier Management Higher-Efficiency...

  16. Strategies for Adapting to Climate Change in Rural Sub-Saharan...

    Open Energy Info (EERE)

    for Adapting to Climate Change in Rural Sub-Saharan Africa Jump to: navigation, search Name Strategies for Adapting to Climate Change in Rural Sub-Saharan Africa AgencyCompany...

  17. Micro-level Practices to Adapt to Climate Change for African...

    Open Energy Info (EERE)

    level Practices to Adapt to Climate Change for African Small-scale Farmers Jump to: navigation, search Name Micro-level Practices to Adapt to Climate Change for African Small-scale...

  18. The adaptive x-ray optics project at the Lawrence Livermore National...

    Office of Scientific and Technical Information (OSTI)

    The adaptive x-ray optics project at the Lawrence Livermore National Laboratory Citation Details In-Document Search Title: The adaptive x-ray optics project at the Lawrence ...

  19. The adaptive x-ray optics project at the Lawrence Livermore National...

    Office of Scientific and Technical Information (OSTI)

    The adaptive x-ray optics project at the Lawrence Livermore National Laboratory Citation Details In-Document Search Title: The adaptive x-ray optics project at the Lawrence Livermore ...

  20. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, Sen Y.

    1989-01-01

    An improvement in a pump including a shrouded inducer, the improvement comprising first and second sealing means 32,36 which cooperate with a first vortex cell 38 and a series of secondary vortex cells 40 to remove any tangential velocity components from the recirculation flow.

  1. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, S.Y.

    1989-08-08

    An improvement in a pump is described including a shrouded inducer, the improvement comprising first and second sealing means which cooperate with a first vortex cell and a series of secondary vortex cells to remove any tangential velocity components from the recirculation flow. 3 figs.

  2. The ADAPT concept - an accelerator driven system for the rapid and

    Office of Scientific and Technical Information (OSTI)

    efficient disposal of plutonium (Journal Article) | SciTech Connect The ADAPT concept - an accelerator driven system for the rapid and efficient disposal of plutonium Citation Details In-Document Search Title: The ADAPT concept - an accelerator driven system for the rapid and efficient disposal of plutonium A new concept; termed ADAPT; for the rapid and virtually complete burning of plutonium is described. ADAPT employs a high current CW linear accelerator (linac) to generate neutrons in a

  3. The Role of Scale and Model Bias in ADAPT's Photospheric Eatimation

    SciTech Connect (OSTI)

    Godinez Vazquez, Humberto C.; Hickmann, Kyle Scott; Arge, Charles Nicholas; Henney, Carl

    2015-05-20

    The Air Force Assimilative Photospheric flux Transport model (ADAPT), is a magnetic flux propagation based on Worden-Harvey (WH) model. ADAPT would be used to provide a global photospheric map of the Earth. A data assimilation method based on the Ensemble Kalman Filter (EnKF), a method of Monte Carlo approximation tied with Kalman filtering, is used in calculating the ADAPT models.

  4. DOE Tour of Zero Floorplans: The Adaptation Home by Evolutionary Home

    Energy Savers [EERE]

    Builders LLC | Department of Energy The Adaptation Home by Evolutionary Home Builders LLC DOE Tour of Zero Floorplans: The Adaptation Home by Evolutionary Home Builders LLC DOE Tour of Zero Floorplans: The Adaptation Home by Evolutionary Home Builders LLC

  5. Demand Response Dispatch Tool

    Energy Science and Technology Software Center (OSTI)

    2012-08-31

    The Demand Response (DR) Dispatch Tool uses price profiles to dispatch demand response resources and create load modifying profiles. These annual profiles are used as inputs to production cost models and regional planning tools (e.g., PROMOD). The tool has been effectively implemented in transmission planning studies conducted by the Western Electricity Coordinating Council via its Transmission Expansion Planning and Policy Committee. The DR Dispatch Tool can properly model the dispatch of DR resources for bothmore » reliability and economic conditions.« less

  6. Roles & Responsibilities | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Roles & Responsibilities PDF icon Roles & Responsibilities.pdf Responsible Contacts Donna Friend HUMAN RESOURCES SPECIALIST E-mail donna.friend@hq.doe.dov Phone 202-586-5880 More ...

  7. Demand Response | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Demand Response Demand Response Demand Response Demand response provides an opportunity for consumers to play a significant role in the operation of the electric grid by reducing or shifting their electricity usage during peak periods in response to time-based rates or other forms of financial incentives. Demand response programs are being used by electric system planners and operators as resource options for balancing supply and demand. Such programs can lower the cost of electricity in

  8. Adaptive Radiotherapy for Head-and-Neck Cancer: Initial Clinical Outcomes From a Prospective Trial

    SciTech Connect (OSTI)

    Schwartz, David L.; Garden, Adam S.; Thomas, Jimmy; Chen Yipei; Zhang Yongbin; Lewin, Jan; Chambers, Mark S.; Dong, Lei

    2012-07-01

    Purpose: To present pilot toxicity and survival outcomes for a prospective trial investigating adaptive radiotherapy (ART) for oropharyngeal squamous cell carcinoma. Methods and Materials: A total of 24 patients were enrolled in an institutional review board-approved clinical trial; data for 22 of these patients were analyzed. Daily CT-guided setup and deformable image registration permitted serial mapping of clinical target volumes and avoidance structures for ART planning. Primary site was base of tongue in 15 patients, tonsil in 6 patient, and glossopharyngeal sulcus in 1 patient. Twenty patients (91%) had American Joint Committee on Cancer (AJCC) Stage IV disease. T stage distribution was 2 T1, 12 T2, 3 T3, 5 T4. N stage distribution was 1 N0, 2 N1, 5 N2a, 12 N2b, and 2 N2c. Of the patients, 21 (95%) received systemic therapy. Results: With a 31-month median follow-up (range, 13-45 months), there has been no primary site failure and 1 nodal relapse, yielding 100% local and 95% regional disease control at 2 years. Baseline tumor size correlated with absolute volumetric treatment response (p = 0.018). Parotid volumetric change correlated with duration of feeding tube placement (p = 0.025). Acute toxicity was comparable to that observed with conventional intensity-modulated radiotherapy (IMRT). Chronic toxicity and functional outcomes beyond 1 year were tabulated. Conclusion: This is the first prospective evaluation of morbidity and survival outcomes in patients with locally advanced head-and-neck cancer treated with automated adaptive replanning. ART can provide dosimetric benefit with only one or two mid-treatment replanning events. Our preliminary clinical outcomes document functional recovery and preservation of disease control at 1-year follow-up and beyond.

  9. Supervisor Responsibilities at Berkeley Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Supervisor Responsibilities at Berkeley Lab This online course will help both new and experienced supervisors better understand key knowledge and responsibilities needed to be an...

  10. General Responsibilities and Requirements

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1999-07-09

    The material presented in this guide provides suggestions and acceptable ways of implementing DOE M 435.1-1 and should not be viewed as additional or mandatory requirements. The objective of the guide is to ensure that responsible individuals understand what is necessary and acceptable for implementing the requirements of DOE M 435.1-1.

  11. Senescence responsive transcriptional element

    DOE Patents [OSTI]

    Campisi, Judith; Testori, Alessandro

    1999-01-01

    Recombinant polynucleotides have expression control sequences that have a senescence responsive element and a minimal promoter, and which are operatively linked to a heterologous nucleotide sequence. The molecules are useful for achieving high levels of expression of genes in senescent cells. Methods of inhibiting expression of genes in senescent cells also are provided.

  12. Laser-Induced Fluorescence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Induced Fluorescence - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs Advanced

  13. Copper deficiency alters cell bioenergetics and induces mitochondrial fusion through up-regulation of MFN2 and OPA1 in erythropoietic cells

    SciTech Connect (OSTI)

    Bustos, Rodrigo I.; Jensen, Erik L.; Ruiz, Lina M.; Rivera, Salvador; Ruiz, Sebastin; Simon, Felipe; Riedel, Claudia; Millennium Institute of Immunology and Immunotherapy, Santiago ; Ferrick, David; Elorza, Alvaro A.

    2013-08-02

    Highlights: In copper deficiency, cell proliferation is not affected. In turn, cell differentiation is impaired. Enlarged mitochondria are due to up-regulation of MNF2 and OPA1. Mitochondria turn off respiratory chain and ROS production. Energy metabolism switch from mitochondria to glycolysis. -- Abstract: Copper is essential in cell physiology, participating in numerous enzyme reactions. In mitochondria, copper is a cofactor for respiratory complex IV, the cytochrome c oxidase. Low copper content is associated with anemia and the appearance of enlarged mitochondria in erythropoietic cells. These findings suggest a connection between copper metabolism and bioenergetics, mitochondrial dynamics and erythropoiesis, which has not been explored so far. Here, we describe that bathocuproine disulfonate-induced copper deficiency does not alter erythropoietic cell proliferation nor induce apoptosis. However it does impair erythroid differentiation, which is associated with a metabolic switch between the two main energy-generating pathways. That is, from mitochondrial function to glycolysis. Switching off mitochondria implies a reduction in oxygen consumption and ROS generation along with an increase in mitochondrial membrane potential. Mitochondrial fusion proteins MFN2 and OPA1 were up-regulated along with the ability of mitochondria to fuse. Morphometric analysis of mitochondria did not show changes in total mitochondrial biomass but rather bigger mitochondria because of increased fusion. Similar results were also obtained with human CD34+, which were induced to differentiate into red blood cells. In all, we have shown that adequate copper levels are important for maintaining proper mitochondrial function and for erythroid differentiation where the energy metabolic switch plus the up-regulation of fusion proteins define an adaptive response to copper deprivation to keep cells alive.

  14. Dosimetrically Triggered Adaptive Intensity Modulated Radiation Therapy for Cervical Cancer

    SciTech Connect (OSTI)

    Lim, Karen; Stewart, James; Kelly, Valerie; Xie, Jason; Brock, Kristy K.; Moseley, Joanne; Cho, Young-Bin; Fyles, Anthony; Lundin, Anna; Rehbinder, Henrik; Lf, Johan; Jaffray, David A.; Milosevic, Michael

    2014-09-01

    Purpose: The widespread use of intensity modulated radiation therapy (IMRT) for cervical cancer has been limited by internal target and normal tissue motion. Such motion increases the risk of underdosing the target, especially as planning margins are reduced in an effort to reduce toxicity. This study explored 2 adaptive strategies to mitigate this risk and proposes a new, automated method that minimizes replanning workload. Methods and Materials: Thirty patients with cervical cancer participated in a prospective clinical study and underwent pretreatment and weekly magnetic resonance (MR) scans over a 5-week course of daily external beam radiation therapy. Target volumes and organs at risk (OARs) were contoured on each of the scans. Deformable image registration was used to model the accumulated dose (the real dose delivered to the target and OARs) for 2 adaptive replanning scenarios that assumed a very small PTV margin of only 3mm to account for setup and internal interfractional motion: (1)a preprogrammed, anatomy-driven midtreatment replan (A-IMRT); and (2) a dosimetry-triggered replan driven by target dose accumulation over time (D-IMRT). Results: Across all 30 patients, clinically relevant target dose thresholds failed for 8 patients (27%) if 3-mm margins were used without replanning. A-IMRT failed in only 3 patients and also yielded an additional small reduction in OAR doses at the cost of 30 replans. D-IMRT assured adequate target coverage in all patients, with only 23 replans in 16 patients. Conclusions: A novel, dosimetry-triggered adaptive IMRT strategy for patients with cervical cancer can minimize the risk of target underdosing in the setting of very small margins and substantial interfractional motion while minimizing programmatic workload and cost.

  15. Adaptive Sampling Algorithms for Probabilistic Risk Assessment of Nuclear Simulations

    SciTech Connect (OSTI)

    Diego Mandelli; Dan Maljovec; Bei Wang; Valerio Pascucci; Peer-Timo Bremer

    2013-09-01

    Nuclear simulations are often computationally expensive, time-consuming, and high-dimensional with respect to the number of input parameters. Thus exploring the space of all possible simulation outcomes is infeasible using finite computing resources. During simulation-based probabilistic risk analysis, it is important to discover the relationship between a potentially large number of input parameters and the output of a simulation using as few simulation trials as possible. This is a typical context for performing adaptive sampling where a few observations are obtained from the simulation, a surrogate model is built to represent the simulation space, and new samples are selected based on the model constructed. The surrogate model is then updated based on the simulation results of the sampled points. In this way, we attempt to gain the most information possible with a small number of carefully selected sampled points, limiting the number of expensive trials needed to understand features of the simulation space. We analyze the specific use case of identifying the limit surface, i.e., the boundaries in the simulation space between system failure and system success. In this study, we explore several techniques for adaptively sampling the parameter space in order to reconstruct the limit surface. We focus on several adaptive sampling schemes. First, we seek to learn a global model of the entire simulation space using prediction models or neighborhood graphs and extract the limit surface as an iso-surface of the global model. Second, we estimate the limit surface by sampling in the neighborhood of the current estimate based on topological segmentations obtained locally. Our techniques draw inspirations from topological structure known as the Morse-Smale complex. We highlight the advantages and disadvantages of using a global prediction model versus local topological view of the simulation space, comparing several different strategies for adaptive sampling in both contexts. One of the most interesting models we propose attempt to marry the two by obtaining a coarse global representation using prediction models, and a detailed local representation based on topology. Our methods are validated on several analytical test functions as well as a small nuclear simulation dataset modeled after a simplified Pressurized Water Reactor.

  16. Model-free adaptive control of advanced power plants

    DOE Patents [OSTI]

    Cheng, George Shu-Xing; Mulkey, Steven L.; Wang, Qiang

    2015-08-18

    A novel 3-Input-3-Output (3.times.3) Model-Free Adaptive (MFA) controller with a set of artificial neural networks as part of the controller is introduced. A 3.times.3 MFA control system using the inventive 3.times.3 MFA controller is described to control key process variables including Power, Steam Throttle Pressure, and Steam Temperature of boiler-turbine-generator (BTG) units in conventional and advanced power plants. Those advanced power plants may comprise Once-Through Supercritical (OTSC) Boilers, Circulating Fluidized-Bed (CFB) Boilers, and Once-Through Supercritical Circulating Fluidized-Bed (OTSC CFB) Boilers.

  17. Comparative study of microwave radiation-induced magnetoresistive oscillations induced by circularly- and linearly- polarized photo-excitation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ye, Tianyu; Liu, Han -Chun; Wang, Zhuo; Wegscheider, W.; Mani, Ramesh G.

    2015-10-09

    A comparative study of the radiation-induced magnetoresistance oscillations in the high mobility GaAs/AlGaAs heterostructure two dimensional electron system (2DES) under linearly- and circularly- polarized microwave excitation indicates a profound difference in the response observed upon rotating the microwave launcher for the two cases, although circularly polarized microwave radiation induced magnetoresistance oscillations observed at low magnetic fields are similar to the oscillations observed with linearly polarized radiation. For the linearly polarized radiation, the magnetoresistive response is a strong sinusoidal function of the launcher rotation (or linear polarization) angle, θ. As a result, for circularly polarized radiation, the oscillatory magnetoresistive response ismore » hardly sensitive to θ.« less

  18. Response Resources Demonstration

    Energy Savers [EERE]

    Interoperability of Demand Response Resources Demonstration in NY Final Technical Report Award Number: DE-FC26-08NT02869 Project Type: Regional Demonstration Principal Investigator: Andre Wellington, Project Manager, Smart Grid Implementation Group Recipient: Consolidated Edison Company of New York, Inc. Team members: Innoventive Power and Verizon Communications Consolidated Edison Company of New York, Inc. Taxpayer ID Number: 13-5009340 Organizational DUNS: 00-698-2359 4 Irving Place New York,

  19. Roles and Responsibilities

    Energy Savers [EERE]

    Partnership Ombudsman - Roles, Responsibilities, Authorities and Accountabilities Prepared by the Technology Partnership Ombudsman The purpose of this document is to provide guidance for the performance of the Technology Partnership Ombudsman function at the national laboratories and facilities within the Department of Energy, and to implement the requirements of the Technology Transfer Commercialization Act of 2000. Each national laboratory is required, and other facilities may be required, to

  20. Climate Change Response

    Energy Savers [EERE]

    the Interior Climate Change Response "From the Everglades to the Great Lakes to Alaska and everywhere in between, climate change is a leading threat to natural and cultural resources across America, and tribal communities are often the hardest hit by severe weather events such as droughts, floods and wildfires" - Secretary of the Interior Sally Jewell "Impacts of climate change are increasingly evident for American Indian and Alaska Native communities and, in some cases, threaten

  1. Structural response synthesis

    SciTech Connect (OSTI)

    Ozisik, H.; Keltie, R.F.

    1988-12-01

    The open loop control technique of predicting a conditioned input signal based on a specified output response for a second order system has been analyzed both analytically and numerically to gain a firm understanding of the method. Differences between this method of control and digital closed loop control using pole cancellation were investigated as a follow up to previous experimental work. Application of the technique to diamond turning using a fast tool is also discussed.

  2. ACCELERATION RESPONSIVE SWITCH

    DOE Patents [OSTI]

    Chabrek, A.F.; Maxwell, R.L.

    1963-07-01

    An acceleration-responsive device with dual channel capabilities whereby a first circuit is actuated upon attainment of a predetermined maximum acceleration level and when the acceleration drops to a predetermined minimum acceleriltion level another circuit is actuated is described. A fluid-damped sensing mass slidably mounted in a relatively frictionless manner on a shaft through the intermediation of a ball bushing and biased by an adjustable compression spring provides inertially operated means for actuating the circuits. (AEC)

  3. Load responsive hydrodynamic bearing

    DOE Patents [OSTI]

    Kalsi, Manmohan S.; Somogyi, Dezso; Dietle, Lannie L.

    2002-01-01

    A load responsive hydrodynamic bearing is provided in the form of a thrust bearing or journal bearing for supporting, guiding and lubricating a relatively rotatable member to minimize wear thereof responsive to relative rotation under severe load. In the space between spaced relatively rotatable members and in the presence of a liquid or grease lubricant, one or more continuous ring shaped integral generally circular bearing bodies each define at least one dynamic surface and a plurality of support regions. Each of the support regions defines a static surface which is oriented in generally opposed relation with the dynamic surface for contact with one of the relatively rotatable members. A plurality of flexing regions are defined by the generally circular body of the bearing and are integral with and located between adjacent support regions. Each of the flexing regions has a first beam-like element being connected by an integral flexible hinge with one of the support regions and a second beam-like element having an integral flexible hinge connection with an adjacent support region. A least one local weakening geometry of the flexing region is located intermediate the first and second beam-like elements. In response to application of load from one of the relatively rotatable elements to the bearing, the beam-like elements and the local weakening geometry become flexed, causing the dynamic surface to deform and establish a hydrodynamic geometry for wedging lubricant into the dynamic interface.

  4. comparative transcriptomics between Synechococcus PCC 7942 and Synechocystis PCC 6803 provide insights into mechanisms of adaptation to stress.

    SciTech Connect (OSTI)

    Billis, Konstantinos; Mavrommatis, Konstantinos; Billini, Maria; Tripp, Harry J.; Kyrpides, Nikos

    2014-03-21

    Background: Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 are model cyanobacteria from which the metabolism and adaptive responses of other cyanobacteria are inferred. Here we report the gene expression response of these two strains to a variety of nutrient and environmental stresses of varying duration, using transcriptomics. Our data comprise both stranded and 5? enriched libraries in order to elucidate many aspects of the transcriptome. Results: Both organisms were exposed to stress conditions due to nutrient deficiency (inorganic carbon) or change of environmental conditions (salinity, temperature, pH, light) sampled at 1 and 24 hours after the application of stress. The transcriptome profile of each strain revealed similarities and differences in gene expression for photosynthetic and respiratory electron transport chains and carbon fixation. Transcriptome profiles also helped us improve the structural annotation of the genome and identify possible missed genes (including anti-sense) and determine transcriptional units (operons). Finally, we predicted association of proteins of unknown function biochemical pathways by associating them to well-characterized ones based on their transcript levels correlation. Conclusions: Overall, this study results an informative annotation of those species and the comparative analysis of the response of the two organisms revealed similarities but also significant changes in the way they respond to external stress and the duration of the response

  5. Adapting ORAP to wind plants : industry value and functional requirements.

    SciTech Connect (OSTI)

    Not Available

    2010-08-01

    Strategic Power Systems (SPS) was contracted by Sandia National Laboratories to assess the feasibility of adapting their ORAP (Operational Reliability Analysis Program) tool for deployment to the wind industry. ORAP for Wind is proposed for use as the primary data source for the CREW (Continuous Reliability Enhancement for Wind) database which will be maintained by Sandia to enable reliability analysis of US wind fleet operations. The report primarily addresses the functional requirements of the wind-based system. The SPS ORAP reliability monitoring system has been used successfully for over twenty years to collect RAM (Reliability, Availability, Maintainability) and operations data for benchmarking and analysis of gas and steam turbine performance. This report documents the requirements to adapt the ORAP system for the wind industry. It specifies which existing ORAP design features should be retained, as well as key new requirements for wind. The latter includes alignment with existing and emerging wind industry standards (IEEE 762, ISO 3977 and IEC 61400). There is also a comprehensive list of thirty critical-to-quality (CTQ) functional requirements which must be considered and addressed to establish the optimum design for wind.

  6. Mining the Agave Microbiome for adaptions to arid environments

    SciTech Connect (OSTI)

    Coleman-Derr, Devin; Wojke, Tanja; North, Gretchen; Partida-Martinez, Laila; DeAngeli, Kristen; Clingenpeel, Scott; Gross, Stephen; Tringe, Susannah; Visel, Axel

    2013-03-25

    A major challenge facing the biofuels industry is the identification of high-yield plant feedstocks that can be cultivated with minimal resource inputs without competing for land and water supplies with existing food crops. Recent research has demonstrated that the Agave plant, cultivated in Mexico and Southwestern United States for the production of fiber and alcohol, meets these criteria1. Agaves grow on non-arable rocky soils in regions characterized by prolonged drought and extreme temperatures, due in part to physiological adaptions that prevent excess water-loss in arid environments2. Plant-microbial symbioses can play a role in helping plants adapt to heat and drought stress, increasing the accessibility of soil nutrients, or compete with plant pathogens3. Whether agaves have similar beneficial microbe interactions in their native environment is unknown. We aim to provide a comprehensive characterization of the Agave microbiome, with the goal of identifying specific community members that may contribute to Agave biotic and abiotic stress tolerance

  7. Limited-memory adaptive snapshot selection for proper orthogonal decomposition

    SciTech Connect (OSTI)

    Oxberry, Geoffrey M.; Kostova-Vassilevska, Tanya; Arrighi, Bill; Chand, Kyle

    2015-04-02

    Reduced order models are useful for accelerating simulations in many-query contexts, such as optimization, uncertainty quantification, and sensitivity analysis. However, offline training of reduced order models can have prohibitively expensive memory and floating-point operation costs in high-performance computing applications, where memory per core is limited. To overcome this limitation for proper orthogonal decomposition, we propose a novel adaptive selection method for snapshots in time that limits offline training costs by selecting snapshots according an error control mechanism similar to that found in adaptive time-stepping ordinary differential equation solvers. The error estimator used in this work is related to theory bounding the approximation error in time of proper orthogonal decomposition-based reduced order models, and memory usage is minimized by computing the singular value decomposition using a single-pass incremental algorithm. Results for a viscous Burgers’ test problem demonstrate convergence in the limit as the algorithm error tolerances go to zero; in this limit, the full order model is recovered to within discretization error. The resulting method can be used on supercomputers to generate proper orthogonal decomposition-based reduced order models, or as a subroutine within hyperreduction algorithms that require taking snapshots in time, or within greedy algorithms for sampling parameter space.

  8. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2003-04-15

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  9. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2008-09-09

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  10. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2005-11-08

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  11. Modeling and simulation of consumer response to dynamic pricing.

    SciTech Connect (OSTI)

    Valenzuela, J.; Thimmapuram, P.; Kim, J (Decision and Information Sciences); (Auburn Univ.)

    2012-08-01

    Assessing the impacts of dynamic-pricing under the smart grid concept is becoming extremely important for deciding its full deployment. In this paper, we develop a model that represents the response of consumers to dynamic pricing. In the model, consumers use forecasted day-ahead prices to shift daily energy consumption from hours when the price is expected to be high to hours when the price is expected to be low while maintaining the total energy consumption as unchanged. We integrate the consumer response model into the Electricity Market Complex Adaptive System (EMCAS). EMCAS is an agent-based model that simulates restructured electricity markets. We explore the impacts of dynamic-pricing on price spikes, peak demand, consumer energy bills, power supplier profits, and congestion costs. A simulation of an 11-node test network that includes eight generation companies and five aggregated consumers is performed for a period of 1 month. In addition, we simulate the Korean power system.

  12. LPG emergency response training

    SciTech Connect (OSTI)

    Dix, R.B.; Newton, B.

    1995-12-31

    ROVER (Roll Over Vehicle for Emergency Response) is a specially designed and constructed unit built to allow emergency response personnel and LPG industry employees to get ``up close and personal`` with the type of equipment used for the highway transportation of liquefied petroleum gas (LPG). This trailer was constructed to simulate an MC 331 LPG trailer. It has all the valves, piping and emergency fittings found on highway tankers. What makes this unit different is that it rolls over and opens up to allow program attendees to climb inside the trailer and see it in a way they have never seen one before. The half-day training session is composed of a classroom portion during which attendees will participate in a discussion of hazardous material safety, cargo tank identification and construction. The specific properties of LPG, and the correct procedures for dealing with an LPG emergency. Attendees will then move outside to ROVER, where they will participate in a walkaround inspection of the rolled over unit. All fittings and piping will be representative of both modern and older equipment. Participants will also be able to climb inside the unit through a specially constructed hatch to view cutaway valves and interior construction. While the possibility of an LPG emergency remains remote, ROVER represents Amoco`s continuing commitment to community, education, and safety.

  13. Predicting the impacts of climate change on animal distributions: the importance of local adaptation and species' traits

    SciTech Connect (OSTI)

    HELLMANN, J. J.; LOBO, N. F.

    2011-12-20

    The geographic range limits of many species are strongly affected by climate and are expected to change under global warming. For species that are able to track changing climate over broad geographic areas, we expect to see shifts in species distributions toward the poles and away from the equator. A number of ecological and evolutionary factors, however, could restrict this shifting or redistribution under climate change. These factors include restricted habitat availability, restricted capacity for or barriers to movement, or reduced abundance of colonists due the perturbation effect of climate change. This research project examined the last of these constraints - that climate change could perturb local conditions to which populations are adapted, reducing the likelihood that a species will shift its distribution by diminishing the number of potential colonists. In the most extreme cases, species ranges could collapse over a broad geographic area with no poleward migration and an increased risk of species extinction. Changes in individual species ranges are the processes that drive larger phenomena such as changes in land cover, ecosystem type, and even changes in carbon cycling. For example, consider the poleward range shift and population outbreaks of the mountain pine beetle that has decimated millions of acres of Douglas fir trees in the western US and Canada. Standing dead trees cause forest fires and release vast quantities of carbon to the atmosphere. The beetle likely shifted its range because it is not locally adapted across its range, and it appears to be limited by winter low temperatures that have steadily increased in the last decades. To understand range and abundance changes like the pine beetle, we must reveal the extent of adaptive variation across species ranges - and the physiological basis of that adaptation - to know if other species will change as readily as the pine beetle. Ecologists tend to assume that range shifts are the dominant response of species to climate change, but our experiments suggest that other processes may act in some species that reduce the likelihood of geographic range change. In the first part of our DOE grant (ending 2008) we argued that the process of local adaptation of populations within a species range, followed by climatic changes that occur too quickly for adaptive evolution, is an underappreciated mechanism by which climate change could affect biodiversity. When this process acts, species ranges may not shift readily toward the poles, slowing the rate of species and biome change. To test this claim, we performed an experiment comparing core and peripheral populations in a series of field observations, translocation experiments, and genetic analyses. The papers in Appendix A were generated from 2005-2008 funding. In the second part of the DOE grant (ending 2011) we studied which traits promote population differentiation and local adaptation by building genomic resources for our study species and using these resources to reveal differences in gene expression in peripheral and core populations. The papers in Appendix B were generated from 2008-2011 funding. This work was pursued with two butterfly species that have contrasting life history traits (body size and resource specialization) and occupy a common ecosystem and a latitudinal range. These species enabled us to test the following hypotheses using a single phylogenetic group.

  14. Application of Advanced Wide Area Early Warning Systems with Adaptive Protection

    SciTech Connect (OSTI)

    Blumstein, Carl; Cibulka, Lloyd; Thorp, James; Centeno, Virgilio; King, Roger; Reeves, Kari; Ashrafi, Frank; Madani, Vahid

    2014-09-30

    Recent blackouts of power systems in North America and throughout the world have shown how critical a reliable power system is to modern societies, and the enormous economic and societal damage a blackout can cause. It has been noted that unanticipated operation of protection systems can contribute to cascading phenomena and, ultimately, blackouts. This project developed and field-tested two methods of Adaptive Protection systems utilizing synchrophasor data. One method detects conditions of system stress that can lead to unintended relay operation, and initiates a supervisory signal to modify relay response in real time to avoid false trips. The second method detects the possibility of false trips of impedance relays as stable system swings “encroach” on the relays’ impedance zones, and produces an early warning so that relay engineers can re-evaluate relay settings. In addition, real-time synchrophasor data produced by this project was used to develop advanced visualization techniques for display of synchrophasor data to utility operators and engineers.

  15. Vibration suppression in cutting tools using collocated piezoelectric sensors/actuators with an adaptive control algorithm

    SciTech Connect (OSTI)

    Radecki, Peter P; Farinholt, Kevin M; Park, Gyuhae; Bement, Matthew T

    2008-01-01

    The machining process is very important in many engineering applications. In high precision machining, surface finish is strongly correlated with vibrations and the dynamic interactions between the part and the cutting tool. Parameters affecting these vibrations and dynamic interactions, such as spindle speed, cut depth, feed rate, and the part's material properties can vary in real-time, resulting in unexpected or undesirable effects on the surface finish of the machining product. The focus of this research is the development of an improved machining process through the use of active vibration damping. The tool holder employs a high bandwidth piezoelectric actuator with an adaptive positive position feedback control algorithm for vibration and chatter suppression. In addition, instead of using external sensors, the proposed approach investigates the use of a collocated piezoelectric sensor for measuring the dynamic responses from machining processes. The performance of this method is evaluated by comparing the surface finishes obtained with active vibration control versus baseline uncontrolled cuts. Considerable improvement in surface finish (up to 50%) was observed for applications in modern day machining.

  16. Adaptation policies to increase terrestrial ecosystem resilience: potential utility of a multicriteria approach

    SciTech Connect (OSTI)

    de Bremond, Ariane; Engle, Nathan L.

    2014-03-01

    Climate change is rapidly undermining terrestrial ecosystem resilience and capacity to continue providing their services to the benefit of humanity and nature. Because of the importance of terrestrial ecosystems to human well-being and supporting services, decision makers throughout the world are busy creating policy responses that secure multiple development and conservation objectives- including that of supporting terrestrial ecosystem resilience in the context of climate change. This article aims to advance analyses on climate policy evaluation and planning in the area of terrestrial ecosystem resilience by discussing adaptation policy options within the ecology-economy-social nexus. The paper evaluates these decisions in the realm of terrestrial ecosystem resilience and evaluates the utility of a set of criteria, indicators, and assessment methods, proposed by a new conceptual multi-criteria framework for pro-development climate policy and planning developed by the United Nations Environment Programme. Potential applications of a multicriteria approach to climate policy vis-A -vis terrestrial ecosystems are then explored through two hypothetical case study examples. The paper closes with a brief discussion of the utility of the multi-criteria approach in the context of other climate policy evaluation approaches, considers lessons learned as a result efforts to evaluate climate policy in the realm of terrestrial ecosystems, and reiterates the role of ecosystem resilience in creating sound policies and actions that support the integration of climate change and development goals.

  17. Managing the risks of extreme events and disasters to advance climate change adaptation. Special report of the Intergovernmental Panel on Climate Change (IPCC)

    SciTech Connect (OSTI)

    Field, C.B.; Barros, V.; Stocker, T.F.

    2012-07-01

    This Special Report on Managing the Risks of Extreme Events and Disasters to Advance Climate Change Adaptation (SREX) has been jointly coordinated by Working Groups I (WGI) and II (WGII) of the Intergovernmental Panel on Climate Change (IPCC). The report focuses on the relationship between climate change and extreme weather and climate events, the impacts of such events, and the strategies to manage the associated risks. This Special Report, in particular, contributes to frame the challenge of dealing with extreme weather and climate events as an issue in decision making under uncertainty, analyzing response in the context of risk management. The report consists of nine chapters, covering risk management; observed and projected changes in extreme weather and climate events; exposure and vulnerability to as well as losses resulting from such events; adaptation options from the local to the international scale; the role of sustainable development in modulating risks; and insights from specific case studies. (LN)

  18. Vulnerability, Sensitivity, and Coping/Adaptive Capacity Worldwide

    SciTech Connect (OSTI)

    Malone, Elizabeth L.; Brenkert, Antoinette L.

    2009-10-01

    Research and analyses have repeatedly shown that impacts of climate change will be unevenly distributed and will affect various societies in various ways. The severity of impacts will depend in part on ability to cope in the short term and adapt in the longer term. However, it has been difficult to find a comparative basis on which to assess differential impacts of climate change. This chapter describes the Vulnerability-Resilience Indicator Model that uses 18 proxy indicators, grouped into 8 elements, to assess on a quantitative basis the comparative potential vulnerability and resilience of countries to climate change. The model integrates socioeconomic and environmental information such as land use, crop production, water availability, per capita GDP, inequality, and health status. Comparative results for 160 countries are presented and analyzed.

  19. Genomic Speciation and Adaptation in Aquilegia (2011 JGI User Meeting)

    ScienceCinema (OSTI)

    Hodges, Scott [University of California, Santa Barbara

    2011-06-03

    The U.S. Department of Energy Joint Genome Institute (JGI) invited scientists interested in the application of genomics to bioenergy and environmental issues, as well as all current and prospective users and collaborators, to attend the annual DOE JGI Genomics of Energy & Environment Meeting held March 22-24, 2011 in Walnut Creek, Calif. The emphasis of this meeting was on the genomics of renewable energy strategies, carbon cycling, environmental gene discovery, and engineering of fuel-producing organisms. The meeting features presentations by leading scientists advancing these topics. Scott Hodges of the University of California, Santa Barbara gives a presentation on "Genomic Speciation and Adaptation in Aquilegia" at the 6th annual Genomics of Energy & Environment Meeting on March 23, 2011

  20. ENZO: AN ADAPTIVE MESH REFINEMENT CODE FOR ASTROPHYSICS

    SciTech Connect (OSTI)

    Bryan, Greg L.; Turk, Matthew J.; Norman, Michael L.; Bordner, James; Xu, Hao; Kritsuk, Alexei G.; O'Shea, Brian W.; Smith, Britton; Abel, Tom; Wang, Peng; Skillman, Samuel W.; Wise, John H.; Reynolds, Daniel R.; Collins, David C.; Harkness, Robert P.; Kim, Ji-hoon; Kuhlen, Michael; Goldbaum, Nathan; Hummels, Cameron; Collaboration: Enzo Collaboration; and others

    2014-04-01

    This paper describes the open-source code Enzo, which uses block-structured adaptive mesh refinement to provide high spatial and temporal resolution for modeling astrophysical fluid flows. The code is Cartesian, can be run in one, two, and three dimensions, and supports a wide variety of physics including hydrodynamics, ideal and non-ideal magnetohydrodynamics, N-body dynamics (and, more broadly, self-gravity of fluids and particles), primordial gas chemistry, optically thin radiative cooling of primordial and metal-enriched plasmas (as well as some optically-thick cooling models), radiation transport, cosmological expansion, and models for star formation and feedback in a cosmological context. In addition to explaining the algorithms implemented, we present solutions for a wide range of test problems, demonstrate the code's parallel performance, and discuss the Enzo collaboration's code development methodology.

  1. Genomic Speciation and Adaptation in Aquilegia (2011 JGI User Meeting)

    SciTech Connect (OSTI)

    Hodges, Scott [University of California, Santa Barbara] [University of California, Santa Barbara

    2011-03-23

    The U.S. Department of Energy Joint Genome Institute (JGI) invited scientists interested in the application of genomics to bioenergy and environmental issues, as well as all current and prospective users and collaborators, to attend the annual DOE JGI Genomics of Energy & Environment Meeting held March 22-24, 2011 in Walnut Creek, Calif. The emphasis of this meeting was on the genomics of renewable energy strategies, carbon cycling, environmental gene discovery, and engineering of fuel-producing organisms. The meeting features presentations by leading scientists advancing these topics. Scott Hodges of the University of California, Santa Barbara gives a presentation on "Genomic Speciation and Adaptation in Aquilegia" at the 6th annual Genomics of Energy & Environment Meeting on March 23, 2011

  2. Boltzmann equation solver adapted to emergent chemical non-equilibrium

    SciTech Connect (OSTI)

    Birrell, Jeremiah; Wilkening, Jon; Rafelski, Johann

    2015-01-15

    We present a novel method to solve the spatially homogeneous and isotropic relativistic Boltzmann equation. We employ a basis set of orthogonal polynomials dynamically adapted to allow for emergence of chemical non-equilibrium. Two time dependent parameters characterize the set of orthogonal polynomials, the effective temperature T(t) and phase space occupation factor ?(t). In this first paper we address (effectively) massless fermions and derive dynamical equations for T(t) and ?(t) such that the zeroth order term of the basis alone captures the particle number density and energy density of each particle distribution. We validate our method and illustrate the reduced computational cost and the ability to easily represent final state chemical non-equilibrium by studying a model problem that is motivated by the physics of the neutrino freeze-out processes in the early Universe, where the essential physical characteristics include reheating from another disappearing particle component (e{sup }-annihilation)

  3. Sweep-twist adaptive rotor blade : final project report.

    SciTech Connect (OSTI)

    Ashwill, Thomas D.

    2010-02-01

    Knight & Carver was contracted by Sandia National Laboratories to develop a Sweep Twist Adaptive Rotor (STAR) blade that reduced operating loads, thereby allowing a larger, more productive rotor. The blade design used outer blade sweep to create twist coupling without angled fiber. Knight & Carver successfully designed, fabricated, tested and evaluated STAR prototype blades. Through laboratory and field tests, Knight & Carver showed the STAR blade met the engineering design criteria and economic goals for the program. A STAR prototype was successfully tested in Tehachapi during 2008 and a large data set was collected to support engineering and commercial development of the technology. This report documents the methodology used to develop the STAR blade design and reviews the approach used for laboratory and field testing. The effort demonstrated that STAR technology can provide significantly greater energy capture without higher operating loads on the turbine.

  4. Network and adaptive system of systems modeling and analysis.

    SciTech Connect (OSTI)

    Lawton, Craig R.; Campbell, James E. Dr.; Anderson, Dennis James; Eddy, John P.

    2007-05-01

    This report documents the results of an LDRD program entitled ''Network and Adaptive System of Systems Modeling and Analysis'' that was conducted during FY 2005 and FY 2006. The purpose of this study was to determine and implement ways to incorporate network communications modeling into existing System of Systems (SoS) modeling capabilities. Current SoS modeling, particularly for the Future Combat Systems (FCS) program, is conducted under the assumption that communication between the various systems is always possible and occurs instantaneously. A more realistic representation of these communications allows for better, more accurate simulation results. The current approach to meeting this objective has been to use existing capabilities to model network hardware reliability and adding capabilities to use that information to model the impact on the sustainment supply chain and operational availability.

  5. Adaptive model predictive process control using neural networks

    DOE Patents [OSTI]

    Buescher, Kevin L.; Baum, Christopher C.; Jones, Roger D.

    1997-01-01

    A control system for controlling the output of at least one plant process output parameter is implemented by adaptive model predictive control using a neural network. An improved method and apparatus provides for sampling plant output and control input at a first sampling rate to provide control inputs at the fast rate. The MPC system is, however, provided with a network state vector that is constructed at a second, slower rate so that the input control values used by the MPC system are averaged over a gapped time period. Another improvement is a provision for on-line training that may include difference training, curvature training, and basis center adjustment to maintain the weights and basis centers of the neural in an updated state that can follow changes in the plant operation apart from initial off-line training data.

  6. Adaptive model predictive process control using neural networks

    DOE Patents [OSTI]

    Buescher, K.L.; Baum, C.C.; Jones, R.D.

    1997-08-19

    A control system for controlling the output of at least one plant process output parameter is implemented by adaptive model predictive control using a neural network. An improved method and apparatus provides for sampling plant output and control input at a first sampling rate to provide control inputs at the fast rate. The MPC system is, however, provided with a network state vector that is constructed at a second, slower rate so that the input control values used by the MPC system are averaged over a gapped time period. Another improvement is a provision for on-line training that may include difference training, curvature training, and basis center adjustment to maintain the weights and basis centers of the neural in an updated state that can follow changes in the plant operation apart from initial off-line training data. 46 figs.

  7. A hyper-spherical adaptive sparse-grid method for high-dimensional

    Office of Scientific and Technical Information (OSTI)

    discontinuity detection (Technical Report) | SciTech Connect Technical Report: A hyper-spherical adaptive sparse-grid method for high-dimensional discontinuity detection Citation Details In-Document Search Title: A hyper-spherical adaptive sparse-grid method for high-dimensional discontinuity detection This work proposes and analyzes a hyper-spherical adaptive hi- erarchical sparse-grid method for detecting jump discontinuities of functions in high-dimensional spaces is proposed. The method

  8. Solution-verified reliability analysis and design of bistable MEMS using error estimation and adaptivity.

    SciTech Connect (OSTI)

    Eldred, Michael Scott; Subia, Samuel Ramirez; Neckels, David; Hopkins, Matthew Morgan; Notz, Patrick K.; Adams, Brian M.; Carnes, Brian; Wittwer, Jonathan W.; Bichon, Barron J.; Copps, Kevin D.

    2006-10-01

    This report documents the results for an FY06 ASC Algorithms Level 2 milestone combining error estimation and adaptivity, uncertainty quantification, and probabilistic design capabilities applied to the analysis and design of bistable MEMS. Through the use of error estimation and adaptive mesh refinement, solution verification can be performed in an automated and parameter-adaptive manner. The resulting uncertainty analysis and probabilistic design studies are shown to be more accurate, efficient, reliable, and convenient.

  9. Time series analysis of Adaptive Optics wave-front sensor telemetry data

    Office of Scientific and Technical Information (OSTI)

    (Technical Report) | SciTech Connect Time series analysis of Adaptive Optics wave-front sensor telemetry data Citation Details In-Document Search Title: Time series analysis of Adaptive Optics wave-front sensor telemetry data Time series analysis techniques are applied to wave-front sensor telemetry data from the Lick Adaptive Optics System. For 28 fully-illuminated subapertures, telemetry data of 4096 consecutive slope estimates for each subaperture are available. The primary problem is

  10. adaptation of DNA repair Byrne, Rose T; Klingele, Audrey J; Cabot...

    Office of Scientific and Technical Information (OSTI)

    Evolution of extreme resistance to ionizing radiation via genetic adaptation of DNA repair Byrne, Rose T; Klingele, Audrey J; Cabot, Eric L; Schackwitz, Wendy S; Martin, Jeffrey A;...

  11. Genomic Analysis of Adaptation and Speciation in Mimulus guttatus (2009 JGI User Meeting)

    ScienceCinema (OSTI)

    Willis, John

    2011-04-25

    John Willis of Duke University spoke about the "Genomic Analysis of Adaptation and Speciation in Mimulus guttatus" on March 26, 2009 at the 4th Annual User Meeting

  12. Climate Change Adaptation Training at HAMMER June 21-22, 2016 | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Climate Change Adaptation Training at HAMMER June 21-22, 2016 Climate Change Adaptation Training at HAMMER June 21-22, 2016 May 11, 2016 - 11:45am Addthis Climate Change Adaptation Training at HAMMER June 21-22, 2016 Climate Change Adaptation Training at HAMMER Executive Order 13693, "Planning for Federal Sustainability in the Next Decade," directs agencies to identify and address projected impacts of climate change on mission critical water, energy, communication and

  13. A hyper-spherical adaptive sparse-grid method for high-dimensional...

    Office of Scientific and Technical Information (OSTI)

    The method is motivated by the the- oretical and computational inefficiencies of well-known adaptive sparse-grid methods for discontinuity detection. Our novel approach constructs ...

  14. Enabling High Efficiency Low Temperature Combustion by Adaptive In-Situ Jet Cooling

    Broader source: Energy.gov [DOE]

    A new approach, called Adaptive-Jet-Cooling, leverages two distinct spray patters of hollow conical sprays and conventional multiple jets, eliminating key sources of NOx and PM.

  15. USING AN ADAPTER TO PERFORM THE CHALFANT-STYLE CONTAINMENT VESSEL PERIODIC MAINTENANCE LEAK RATE TEST

    SciTech Connect (OSTI)

    Loftin, B.; Abramczyk, G.; Trapp, D.

    2011-06-03

    Recently the Packaging Technology and Pressurized Systems (PT&PS) organization at the Savannah River National Laboratory was asked to develop an adapter for performing the leak-rate test of a Chalfant-style containment vessel. The PT&PS organization collaborated with designers at the Department of Energy's Pantex Plant to develop the adapter currently in use for performing the leak-rate testing on the containment vessels. This paper will give the history of leak-rate testing of the Chalfant-style containment vessels, discuss the design concept for the adapter, give an overview of the design, and will present results of the testing done using the adapter.

  16. iRESM INITIATIVE UNDERSTANDING DECISION SUPPORT NEEDS FOR CLIMATE CHANGE MITIGATION AND ADAPTATION --US Midwest Region—

    SciTech Connect (OSTI)

    Rice, Jennie S.; Runci, Paul J.; Moss, Richard H.; Anderson, Kate L.

    2010-10-01

    The impacts of climate change are already affecting human and environmental systems worldwide, yet many uncertainties persist in the prediction of future climate changes and impacts due to limitations in scientific understanding of relevant causal factors. In particular, there is mounting urgency to efforts to improve models of human and environmental systems at the regional scale, and to integrate climate, ecosystem and energy-economic models to support policy, investment, and risk management decisions related to climate change mitigation (i.e., reducing greenhouse gas emissions) and adaptation (i.e., responding to climate change impacts). The Pacific Northwest National Laboratory (PNNL) is developing a modeling framework, the integrated Regional Earth System Model (iRESM), to address regional human-environmental system interactions in response to climate change and the uncertainties therein. The framework will consist of a suite of integrated models representing regional climate change, regional climate policy, and the regional economy, with a focus on simulating the mitigation and adaptation decisions made over time in the energy, transportation, agriculture, and natural resource management sectors.

  17. Technology Partnership Ombudsman - Roles, Responsibilities, Authoritie...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technology Partnership Ombudsman - Roles, Responsibilities, Authorities and Accountabilities Technology Partnership Ombudsman - Roles, Responsibilities, Authorities and ...

  18. Tunable and adaptive bandpass filter using a nonlinear dielectric thin film of SrTiO{sub 3}

    SciTech Connect (OSTI)

    Findikoglu, A.T.; Jia, Q.X.; Wu, X.D.; Chen, G.J.; Venkatesan, T.; Reagor, D.W.

    1996-03-01

    We have prepared an electrically tunable and adaptive 3-pole half-wave bandpass coplanar waveguide filter incorporating a 1.2-{mu}m-thick paraelectric SrTiO{sub 3} bottom layer and a 0.4-{mu}m-thick superconducting YBa{sub 2}Cu{sub 3}O{sub 7{minus}{ital x}} top electrode layer on a LaAlO{sub 3} substrate. By applying a separate bias voltage on each pole and also on each coupling capacitance of the device at 4 and 76 K, the filter response is not only fine tuned to achieve symmetric and optimized filter characteristics with less than 2{percent} bandwidth centered around 2.5 GHz, but also broadband tuned to shift the passband by more than 15{percent}. {copyright} {ital 1996 American Institute of Physics.}

  19. An Adaptive Landscape Classification Procedure using Geoinformatics and Artificial Neural Networks

    SciTech Connect (OSTI)

    Coleman, Andre M.

    2008-08-01

    The Adaptive Landscape Classification Procedure (ALCP), which links the advanced geospatial analysis capabilities of Geographic Information Systems (GISs) and Artificial Neural Networks (ANNs) and particularly Self-Organizing Maps (SOMs), is proposed as a method for establishing and reducing complex data relationships. Its adaptive and evolutionary capability is evaluated for situations where varying types of data can be combined to address different prediction and/or management needs such as hydrologic response, water quality, aquatic habitat, groundwater recharge, land use, instrumentation placement, and forecast scenarios. The research presented here documents and presents favorable results of a procedure that aims to be a powerful and flexible spatial data classifier that fuses the strengths of geoinformatics and the intelligence of SOMs to provide data patterns and spatial information for environmental managers and researchers. This research shows how evaluation and analysis of spatial and/or temporal patterns in the landscape can provide insight into complex ecological, hydrological, climatic, and other natural and anthropogenic-influenced processes. Certainly, environmental management and research within heterogeneous watersheds provide challenges for consistent evaluation and understanding of system functions. For instance, watersheds over a range of scales are likely to exhibit varying levels of diversity in their characteristics of climate, hydrology, physiography, ecology, and anthropogenic influence. Furthermore, it has become evident that understanding and analyzing these diverse systems can be difficult not only because of varying natural characteristics, but also because of the availability, quality, and variability of spatial and temporal data. Developments in geospatial technologies, however, are providing a wide range of relevant data, and in many cases, at a high temporal and spatial resolution. Such data resources can take the form of high-dimensional data arrays, which can difficult to fully use. Establishing relationships among high-dimensional datasets through neurocomputing based patterning methods can help 1) resolve large volumes of data into a meaningful form; 2) provide an approach for inferring landscape processes in areas that have limited data available but that exhibit similar landscape characteristics; and 3) discover the value of individual variables or groups of variables that contribute to specific processes in the landscape.

  20. SU-E-J-254: Utility of Pinnacle Dynamic Planning Module Utilizing Deformable Image Registration in Adaptive Radiotherapy

    SciTech Connect (OSTI)

    Jani, S

    2014-06-01

    Purpose For certain highly conformal treatment techniques, changes in patient anatomy due to weight loss and/or tumor shrinkage can result in significant changes in dose distribution. Recently, the Pinnacle treatment planning system added a Dynamic Planning module utilizing Deformable Image Registration (DIR). The objective of this study was to evaluate the effectiveness of this software in adapting to altered anatomy and adjusting treatment plans to account for it. Methods We simulated significant tumor response by changing patient thickness and altered chin positions using a commercially-available head and neck (H and N) phantom. In addition, we studied 23 CT image sets of fifteen (15) patients with H and N tumors and eight (8) patients with prostate cancer. In each case, we applied deformable image registration through Dynamic Planning module of our Pinnacle Treatment Planning System. The dose distribution of the original CT image set was compared to the newly computed dose without altering any treatment parameter. Result was a dose if we did not adjust the plan to reflect anatomical changes. Results For the H and N phantom, a tumor response of up to 3.5 cm was correctly deformed by the Pinnacle Dynamic module. Recomputed isodose contours on new anatomies were within 1 mm of the expected distribution. The Pinnacle system configuration allowed dose computations resulting from original plans on new anatomies without leaving the planning system. Original and new doses were available side-by-side with both CT image sets. Based on DIR, about 75% of H and N patients (11/15) required a re-plan using new anatomy. Among prostate patients, the DIR predicted near-correct bladder volume in 62% of the patients (5/8). Conclusions The Dynamic Planning module of the Pinnacle system proved to be an accurate and useful tool in our ability to adapt to changes in patient anatomy during a course of radiotherapy.

  1. An adaptive filtered back-projection for photoacoustic image reconstruction

    SciTech Connect (OSTI)

    Huang, He; Bustamante, Gilbert; Peterson, Ralph; Ye, Jing Yong

    2015-05-15

    Purpose: The purpose of this study is to develop an improved filtered-back-projection (FBP) algorithm for photoacoustic tomography (PAT), which allows image reconstruction with higher quality compared to images reconstructed through traditional algorithms. Methods: A rigorous expression of a weighting function has been derived directly from a photoacoustic wave equation and used as a ramp filter in Fourier domain. The authors new algorithm utilizes this weighting function to precisely calculate each photoacoustic signals contribution and then reconstructs the image based on the retarded potential generated from the photoacoustic sources. In addition, an adaptive criterion has been derived for selecting the cutoff frequency of a low pass filter. Two computational phantoms were created to test the algorithm. The first phantom contained five spheres with each sphere having different absorbances. The phantom was used to test the capability for correctly representing both the geometry and the relative absorbed energy in a planar measurement system. The authors also used another phantom containing absorbers of different sizes with overlapping geometry to evaluate the performance of the new method for complicated geometry. In addition, random noise background was added to the simulated data, which were obtained by using an arc-shaped array of 50 evenly distributed transducers that spanned 160 over a circle with a radius of 65 mm. A normalized factor between the neighbored transducers was applied for correcting measurement signals in PAT simulations. The authors assumed that the scanned object was mounted on a holder that rotated over the full 360 and the scans were set to a sampling rate of 20.48 MHz. Results: The authors have obtained reconstructed images of the computerized phantoms by utilizing the new FBP algorithm. From the reconstructed image of the first phantom, one can see that this new approach allows not only obtaining a sharp image but also showing the correct signal strength of the absorbers. The reconstructed image of the second phantom further demonstrates the capability to form clear images of the spheres with sharp borders in the overlapping geometry. The smallest sphere is clearly visible and distinguishable, even though it is surrounded by two big spheres. In addition, image reconstructions were conducted with randomized noise added to the observed signals to mimic realistic experimental conditions. Conclusions: The authors have developed a new FBP algorithm that is capable for reconstructing high quality images with correct relative intensities and sharp borders for PAT. The results demonstrate that the weighting function serves as a precise ramp filter for processing the observed signals in the Fourier domain. In addition, this algorithm allows an adaptive determination of the cutoff frequency for the applied low pass filter.

  2. Temporal Scattering And Response

    Energy Science and Technology Software Center (OSTI)

    1992-12-15

    TSAR2.3 (Temporal Scattering and Response) is a finite-difference time-domain electromagnetics code suite. TSAR2.3 is a software package for simulating the interactions of electromagnetic waves with linear materials through the use of the finite-difference time-domain method. The code suite contains grid generation, grid verification, input-file creation and post-processing utilities. The physics package, written in Fortran 77, can be pre-processed to run on many different architectures including Cray, Vax and many Unix workstations. Tools are provided tomore » easily port the code to new computers. The physics package is an efficient, flexible electromagnetic simulator. A body under study can be represented as a three-dimensional grid of materials with arbitrary linear properties. This grid can be simulated in a number of ways including incident plane waves, dipoles, and arbitrary incident fields. The grid can be terminated with numerous boundary conditions including free-space radiation, electric conductor, or magnetic conductor. Projection to the far-field in both the time and frequency domains is possible. This distribution includes make files for installing and maintaining the entire code suite.« less

  3. Emergency Response Health Physics

    SciTech Connect (OSTI)

    Mena, RaJah; Pemberton, Wendy; Beal, William

    2012-05-01

    Health physics is an important discipline with regard to understanding the effects of radiation on human health; however, there are major differences between health physics for research or occupational safety and health physics during a large-scale radiological emergency. The deployment of a U.S. Department of Energy/National Nuclear Security Administration (DOE/NNSA) monitoring and assessment team to Japan in the wake of the March 2011 accident at Fukushima Daiichi Nuclear Power Plant yielded a wealth of lessons on these difference. Critical teams (CMOC (Consequence Management Outside the Continental U.S.) and CMHT (Consequence Management Home Team) ) worked together to collect, compile, review, and analyze radiological data from Japan to support the response needs of and answer questions from the Government of Japan, the U.S. military in Japan, the U.S. Embassy and U.S. citizens in Japan, and U.S. citizens in America. This paper addresses the unique challenges presented to the health physicist or analyst of radiological data in a large-scale emergency. A key lesson learned was that public perception and the availability of technology with social media requires a diligent effort to keep the public informed of the science behind the decisions in a manner that is meaningful to them.

  4. 3D, Flash, Induced Current Readout for Silicon Sensors

    SciTech Connect (OSTI)

    Parker, Sherwood I.

    2014-06-07

    A new method for silicon microstrip and pixel detector readout using (1) 65 nm-technology current amplifers which can, for the first time with silicon microstrop and pixel detectors, have response times far shorter than the charge collection time (2) 3D trench electrodes large enough to subtend a reasonable solid angle at most track locations and so have adequate sensitivity over a substantial volume of pixel, (3) induced signals in addition to, or in place of, collected charge

  5. Gamma Detector Response and Analysis Software - Detector Response Function

    Energy Science and Technology Software Center (OSTI)

    2014-05-13

    GADRAS-DRF uses a Detector Response Function (DRF) to compute the response of gamma-ray detectors incident radiation. The application includes provision for plotting measured and computed spectra and for characterizing detector response parameters based on measurements of a series of calibration sources (e.g., Ba-133, Cs-137, Co-60, and Th-228). An application program interface enables other programs to access the dynamic-link library that is used to compute spectra.

  6. Adaptive immunity and histopathology in frog virus 3-infected Xenopus

    SciTech Connect (OSTI)

    Robert, Jacques . E-mail: robert@mail.rochester.edu; Morales, Heidi; Buck, Wayne; Cohen, Nicholas; Marr, Shauna; Gantress, Jennifer

    2005-02-20

    Xenopus has been used as an experimental model to evaluate the contribution of adaptive cellular immunity in amphibian host susceptibility to the emerging ranavirus FV3. Conventional histology and immunohistochemistry reveal that FV3 has a strong tropism for the proximal tubular epithelium of the kidney and is rarely disseminated elsewhere in Xenopus hosts unless their immune defenses are impaired or developmentally immature as in larvae. In such cases, virus is found widespread in most tissues. Adults, immunocompromised by depletion of CD8{sup +} T cells or by sub-lethal {gamma}-irradiation, show increased susceptibility to FV3 infection. Larvae and irradiated (but not normal) adults can be cross-infected through water by infected adult conspecifics (irradiated or not). The natural MHC class I deficiency and the absence of effect of anti-CD8 treatment on both larval CD8{sup +} T cells and larval susceptibility to FV3 are consistent with an inefficient CD8{sup +} T cell effector function during this developmental period.

  7. Towards adaptive, streaming analysis of x-ray tomography data

    SciTech Connect (OSTI)

    Thomas, Mathew; Kleese van Dam, Kerstin; Marshall, Matthew J.; Kuprat, Andrew P.; Carson, James P.; Lansing, Carina S.; Guillen, Zoe C.; Miller, Erin A.; Lanekoff, Ingela; Laskin, Julia

    2015-03-04

    Temporal and spatial resolution of chemical imaging methodologies such as x-ray tomography are rapidly increasing, leading to more complex experimental procedures and fast growing data volumes. Automated analysis pipelines and big data analytics are becoming essential to effectively evaluate the results of such experiments. Offering those data techniques in an adaptive, streaming environment can further substantially improve the scientific discovery process, by enabling experimental control and steering based on the evaluation of emerging phenomena as they are observed by the experiment. Pacific Northwest National Laboratory (PNNL)’ Chemical Imaging Initiative (CII - http://imaging.pnnl.gov/ ) has worked since 2011 towards developing a framework that allows users to rapidly compose and customize high throughput experimental analysis pipelines for multiple instrument types. The framework, named ‘Rapid Experimental Analysis’ (REXAN) Framework [1], is based on the idea of reusable component libraries and utilizes the PNNL developed collaborative data management and analysis environment ‘Velo’, to provide a user friendly analysis and data management environment for experimental facilities. This article will, discuss the capabilities established for X-Ray tomography, discuss lessons learned, and provide an overview of our more recent work in the Analysis in Motion Initiative (AIM - http://aim.pnnl.gov/ ) at PNNL to provide REXAN capabilities in a streaming environment.

  8. Evolutionary Adaptive Discovery of Phased Array Sensor Signal Identification

    SciTech Connect (OSTI)

    Timothy R. McJunkin; Milos Manic

    2011-05-01

    Tomography, used to create images of the internal properties and features of an object, from phased array ultasonics is improved through many sophisiticated methonds of post processing of data. One approach used to improve tomographic results is to prescribe the collection of more data, from different points of few so that data fusion might have a richer data set to work from. This approach can lead to rapid increase in the data needed to be stored and processed. It also does not necessarily lead to have the needed data. This article describes a novel approach to utilizing the data aquired as a basis for adapting the sensors focusing parameters to locate more precisely the features in the material: specifically, two evolutionary methods of autofocusing on a returned signal are coupled with the derivations of the forumulas for spatially locating the feature are given. Test results of the two novel methods of evolutionary based focusing (EBF) illustrate the improved signal strength and correction of the position of feature using the optimized focal timing parameters, called Focused Delay Identification (FoDI).

  9. Radiation effects in the stainless steel primary coolant supply adapter

    SciTech Connect (OSTI)

    Farrell, K.

    1995-09-01

    The primary coolant supply adapter (PCSA) is a flanged, cylindrical collar of 316NG stainless steel that is part of the primary pressure boundary of the Advanced Neutron Source. The radiation fluxes on the PCSA are dominated by thermal neutrons. During its intended 40-year service life, the PCSA will receive a thermal neutron fluence of 1.8 {times} 10{sup 26} m{sup {minus}2} in its upper sections at a temperature of <1OO{degree}C. The PCSA will suffer radiation damage, caused primarily by the interaction of thermal neutrons with the 14% nickel in the steel, which will generate helium by the sequential reactions {sup 58}Ni (n,y){sup 59}Ni (n,{alpha}){sup 56}Fe and will concurrently produce significant atomic displacements per atom (dpa) from the {sup 59}Ni (n,{alpha}){sup 56}Fe recoils. It is estimated that the helium concentration and total atomic displacements in the upper parts of the PCSA will be about 430 atomic parts per million and 1 dpa, respectively. From newly compiled trend curves of tensile properties and fracture toughness data versus atomic displacements for 316 steel, it is deduced that the irradiated PCSA will retain at least 20% uniform tensile elongation and a fracture toughness of more than 200 Mpa{radical}m, which are judged adequate to resist brittle failure. Tberefore, employment of a neutron shield around the PCSA is unnecessary.

  10. Parallel Block Structured Adaptive Mesh Refinement on Graphics Processing Units

    SciTech Connect (OSTI)

    Beckingsale, D. A.; Gaudin, W. P.; Hornung, R. D.; Gunney, B. T.; Gamblin, T.; Herdman, J. A.; Jarvis, S. A.

    2014-11-17

    Block-structured adaptive mesh refinement is a technique that can be used when solving partial differential equations to reduce the number of zones necessary to achieve the required accuracy in areas of interest. These areas (shock fronts, material interfaces, etc.) are recursively covered with finer mesh patches that are grouped into a hierarchy of refinement levels. Despite the potential for large savings in computational requirements and memory usage without a corresponding reduction in accuracy, AMR adds overhead in managing the mesh hierarchy, adding complex communication and data movement requirements to a simulation. In this paper, we describe the design and implementation of a native GPU-based AMR library, including: the classes used to manage data on a mesh patch, the routines used for transferring data between GPUs on different nodes, and the data-parallel operators developed to coarsen and refine mesh data. We validate the performance and accuracy of our implementation using three test problems and two architectures: an eight-node cluster, and over four thousand nodes of Oak Ridge National Laboratory’s Titan supercomputer. Our GPU-based AMR hydrodynamics code performs up to 4.87× faster than the CPU-based implementation, and has been scaled to over four thousand GPUs using a combination of MPI and CUDA.

  11. Troglitazone induces differentiation in Trypanosoma brucei

    SciTech Connect (OSTI)

    Denninger, Viola; Figarella, Katherine; Schoenfeld, Caroline; Brems, Stefanie; Busold, Christian; Lang, Florian; Hoheisel, Joerg; Duszenko, Michael . E-mail: michael.duszenko@uni-tuebingen.de

    2007-05-15

    Trypanosoma brucei, a protozoan parasite causing sleeping sickness, is transmitted by the tsetse fly and undergoes a complex lifecycle including several defined stages within the insect vector and its mammalian host. In the latter, differentiation from the long slender to the short stumpy form is induced by a yet unknown factor of trypanosomal origin. Here we describe that some thiazolidinediones are also able to induce differentiation. In higher eukaryotes, thiazolidinediones are involved in metabolism and differentiation processes mainly by binding to the intracellular receptor peroxisome proliferator activated receptor {gamma}. Our studies focus on the effects of troglitazone on bloodstream form trypanosomes. Differentiation was monitored using mitochondrial markers (membrane potential, succinate dehydrogenase activity, inhibition of oxygen uptake by KCN, amount of cytochrome transcripts), morphological changes (Transmission EM and light microscopy), and transformation experiments (loss of the Variant Surface Glycoprotein coat and increase of dihydroliponamide dehydrogenase activity). To further investigate the mechanisms responsible for these changes, microarray analyses were performed, showing an upregulation of expression site associated gene 8 (ESAG8), a potential differentiation regulator.

  12. Climate change effects on agriculture: Economic responses to biophysical shocks

    SciTech Connect (OSTI)

    Nelson, Gerald; Valin, Hugo; Sands, Ronald; Havlik, Petr; Ahammad, Helal; Deryng, Delphine; Elliott, Joshua; Fujimori, Shinichiro; Hasegawa, Tomoko; Heyhoe, Edwina; Kyle, G. Page; von Lampe, Martin; Lotze-Campen, Hermann; Mason d'Croz, Daniel; van Meijl, Hans; van der Mensbrugghe, Dominique; Mueller, C.; Popp, Alexander; Robertson, Richard; Robinson, Sherman; Schmid, E.; Schmitz, Christoph; Tabeau, Andrzej; Willenbockel, Dirk

    2013-12-16

    Agricultural production is sensitive to weather and will thus be directly affected by climate change. Plausible estimates of these climate change impacts require combined use of climate, crop, and economic models. Results from previous studies vary substantially due to differences in models, scenarios, and data. This paper is part of a collective effort to systematically integrate these three types of models. We focus on the economic component of the assessment, investigating how nine global economic models of agriculture represent endogenous responses to seven standardized climate change scenarios produced by two climate and five crop models. These responses include adjustments in yields, area, consumption, and international trade. We apply biophysical shocks derived from the IPCC’s Representative Concentration Pathway that result in end-of-century radiative forcing of 8.5 watts per square meter. The mean biophysical impact on crop yield with no incremental CO2 fertilization is a 17 percent reduction globally by 2050 relative to a scenario with unchanging climate. Endogenous economic responses reduce yield loss to 11 percent, increase area of major crops by 12 percent, and reduce consumption by 2 percent. Agricultural production, cropland area, trade, and prices show the greatest degree of variability in response to climate change, and consumption the lowest. The sources of these differences includes model structure and specification; in particular, model assumptions about ease of land use conversion, intensification, and trade. This study identifies where models disagree on the relative responses to climate shocks and highlights research activities needed to improve the representation of agricultural adaptation responses to climate change.

  13. Demand Response Programs, 6. edition

    SciTech Connect (OSTI)

    2007-10-15

    The report provides a look at the past, present, and future state of the market for demand/load response based upon market price signals. It is intended to provide significant value to individuals and companies who are considering participating in demand response programs, energy providers and ISOs interested in offering demand response programs, and consultants and analysts looking for detailed information on demand response technology, applications, and participants. The report offers a look at the current Demand Response environment in the energy industry by: defining what demand response programs are; detailing the evolution of program types over the last 30 years; discussing the key drivers of current initiatives; identifying barriers and keys to success for the programs; discussing the argument against subsidization of demand response; describing the different types of programs that exist including:direct load control, interruptible load, curtailable load, time-of-use, real time pricing, and demand bidding/buyback; providing examples of the different types of programs; examining the enablers of demand response programs; and, providing a look at major demand response programs.

  14. Demand Response Technology Roadmap A

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    meetings and workshops convened to develop content for the Demand Response Technology Roadmap. The project team has developed this companion document in the interest of providing...

  15. Deepwater_Response.pdf | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DeepwaterResponse.pdf DeepwaterResponse.pdf PDF icon DeepwaterResponse.pdf More Documents & Publications UDAC Meeting - September 2012 UDAC Meeting - January 2012...

  16. BPA-2013-01679-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    following: "BPA's response to the Redman Report, which was provided to Public Power Council (PPC) in February of 2008." Response: BPA is releasing the enclosed responsive...

  17. Structural and functional adaptation of vancomycin resistance VanT serine racemases

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Meziane-Cherif, Djalal; Stogios, Peter J.; Evdokimova, Elena; Egorova, Olga; Savchenko, Alexei; Courvalin, Patrice

    2015-08-11

    Vancomycin resistance in Gram-positive bacteria results from the replacement of the D-alanyl–D-alanine target of peptidoglycan precursors with D-alanyl–D-lactate or D-alanyl–D-serine (D-Ala-D-Ser), to which vancomycin has low binding affinity. VanT is one of the proteins required for the production of D-Ala-D-Ser-terminating precursors by converting L-Ser to D-Ser. VanT is composed of two domains, an N-terminal membrane-bound domain, likely involved in L-Ser uptake, and a C-terminal cytoplasmic catalytic domain which is related to bacterial alanine racemases. To gain insight into the molecular function of VanT, the crystal structure of the catalytic domain of VanTG from VanG-type resistant Enterococcus faecalis BM4518 wasmore » determined. The structure showed significant similarity to type III pyridoxal 5'-phosphate (PLP)-dependent alanine racemases, which are essential for peptidoglycan synthesis. Comparative structural analysis between VanTG and alanine racemases as well as site-directed mutagenesis identified three specific active site positions centered around Asn696 which are responsible for theL-amino acid specificity. This analysis also suggested that VanT racemases evolved from regular alanine racemases by acquiring additional selectivity toward serine while preserving that for alanine. The 4-fold-lower relative catalytic efficiency of VanTG against L-Ser versus L-Ala implied that this enzyme relies on its membrane-bound domain for L-Ser transport to increase the overall rate of D-Ser production. These findings illustrate how vancomycin pressure selected for molecular adaptation of a housekeeping enzyme to a bifunctional enzyme to allow for peptidoglycan remodeling, a strategy increasingly observed in antibiotic-resistant bacteria.« less

  18. Structural and functional adaptation of vancomycin resistance VanT serine racemases

    SciTech Connect (OSTI)

    Meziane-Cherif, Djalal; Stogios, Peter J.; Evdokimova, Elena; Egorova, Olga; Savchenko, Alexei; Courvalin, Patrice

    2015-08-11

    Vancomycin resistance in Gram-positive bacteria results from the replacement of the D-alanyl–D-alanine target of peptidoglycan precursors with D-alanyl–D-lactate or D-alanyl–D-serine (D-Ala-D-Ser), to which vancomycin has low binding affinity. VanT is one of the proteins required for the production of D-Ala-D-Ser-terminating precursors by converting L-Ser to D-Ser. VanT is composed of two domains, an N-terminal membrane-bound domain, likely involved in L-Ser uptake, and a C-terminal cytoplasmic catalytic domain which is related to bacterial alanine racemases. To gain insight into the molecular function of VanT, the crystal structure of the catalytic domain of VanTG from VanG-type resistant Enterococcus faecalis BM4518 was determined. The structure showed significant similarity to type III pyridoxal 5'-phosphate (PLP)-dependent alanine racemases, which are essential for peptidoglycan synthesis. Comparative structural analysis between VanTG and alanine racemases as well as site-directed mutagenesis identified three specific active site positions centered around Asn696 which are responsible for theL-amino acid specificity. This analysis also suggested that VanT racemases evolved from regular alanine racemases by acquiring additional selectivity toward serine while preserving that for alanine. The 4-fold-lower relative catalytic efficiency of VanTG against L-Ser versus L-Ala implied that this enzyme relies on its membrane-bound domain for L-Ser transport to increase the overall rate of D-Ser production. These findings illustrate how vancomycin pressure selected for molecular adaptation of a housekeeping enzyme to a bifunctional enzyme to allow for peptidoglycan remodeling, a strategy increasingly observed in antibiotic-resistant bacteria.

  19. Performance Bounds on Micro-Doppler Estimation and Adaptive Waveform Design Using OFDM Signals

    SciTech Connect (OSTI)

    Sen, Satyabrata; Barhen, Jacob; Glover, Charles Wayne

    2014-01-01

    We analyze the performance of a wideband orthogonal frequency division multiplexing (OFDM) signal in estimating the micro-Doppler frequency of a target having multiple rotating scatterers (e.g., rotor blades of a helicopter, propellers of a submarine). The presence of rotating scatterers introduces Doppler frequency modulation in the received signal by generating sidebands about the transmitted frequencies. This is called the micro-Doppler effects. The use of a frequency-diverse OFDM signal in this context enables us to independently analyze the micro-Doppler characteristics with respect to a set of orthogonal subcarrier frequencies. Therefore, to characterize the accuracy of micro-Doppler frequency estimation, we compute the Cram er-Rao Bound (CRB) on the angular-velocity estimate of the target while considering the scatterer responses as deterministic but unknown nuisance parameters. Additionally, to improve the accuracy of the estimation procedure, we formulate and solve an optimization problem by minimizing the CRB on the angular-velocity estimate with respect to the transmitting OFDM spectral coefficients. We present several numerical examples to demonstrate the CRB variations at different values of the signal-to-noise ratio (SNR) and the number of OFDM subcarriers. The CRB values not only decrease with the increase in the SNR values, but also reduce as we increase the number of subcarriers implying the significance of frequency-diverse OFDM waveforms. The improvement in estimation accuracy due to the adaptive waveform design is also numerically analyzed. Interestingly, we find that the relative decrease in the CRBs on the angular-velocity estimate is more pronounced for larger number of OFDM subcarriers.

  20. RAM: a Relativistic Adaptive Mesh Refinement Hydrodynamics Code

    SciTech Connect (OSTI)

    Zhang, Wei-Qun; MacFadyen, Andrew I.; /Princeton, Inst. Advanced Study

    2005-06-06

    The authors have developed a new computer code, RAM, to solve the conservative equations of special relativistic hydrodynamics (SRHD) using adaptive mesh refinement (AMR) on parallel computers. They have implemented a characteristic-wise, finite difference, weighted essentially non-oscillatory (WENO) scheme using the full characteristic decomposition of the SRHD equations to achieve fifth-order accuracy in space. For time integration they use the method of lines with a third-order total variation diminishing (TVD) Runge-Kutta scheme. They have also implemented fourth and fifth order Runge-Kutta time integration schemes for comparison. The implementation of AMR and parallelization is based on the FLASH code. RAM is modular and includes the capability to easily swap hydrodynamics solvers, reconstruction methods and physics modules. In addition to WENO they have implemented a finite volume module with the piecewise parabolic method (PPM) for reconstruction and the modified Marquina approximate Riemann solver to work with TVD Runge-Kutta time integration. They examine the difficulty of accurately simulating shear flows in numerical relativistic hydrodynamics codes. They show that under-resolved simulations of simple test problems with transverse velocity components produce incorrect results and demonstrate the ability of RAM to correctly solve these problems. RAM has been tested in one, two and three dimensions and in Cartesian, cylindrical and spherical coordinates. they have demonstrated fifth-order accuracy for WENO in one and two dimensions and performed detailed comparison with other schemes for which they show significantly lower convergence rates. Extensive testing is presented demonstrating the ability of RAM to address challenging open questions in relativistic astrophysics.

  1. Laser Induced Spectroscopy - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laser Induced Spectroscopy Idaho National Laboratory Contact INL About This Technology Technology Marketing Summary INL's Laser Induced Spectroscopy technology detects and measures ...

  2. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Disorder-Induced Microscopic Magnetic Memory Print Wednesday, 26 October 2005 00:00 The magnetic-recording industry deliberately...

  3. Proteome Analysis of Borrelia burgdorferi Response to Environmental Change

    SciTech Connect (OSTI)

    Angel, Thomas E.; Luft, Benjamin J.; Yang, Xiaohua; Nicora, Carrie D.; Camp, David G.; Jacobs, Jon M.; Smith, Richard D.

    2010-11-02

    We examined global changes in protein expression in the B31 strain of Borrelia burgdorferi, in response to two environmental cues (pH and temperature) chosen for their reported similarity to those encountered at different stages of the organisms life cycle. Multidimensional nano-liquid chromatographic separations coupled with tandem mass spectrometry were used to examine the array of proteins (i.e., the proteome) of B. burgdorferi for different pH and temperature culture conditions. Changes in pH and temperature elicited in vitro adaptations of this spirochete known to cause Lyme disease and led to alterations in protein expression that are associated with increased microbial pathogenesis. We identified 1031 proteins that represent 59% of the annotated genome of B. burgdorferi and elucidated a core proteome of 414 proteins that were present in all environmental conditions investigated. Observed changes in protein abundances indicated varied replicon usage, as well as proteome functional distributions between the in vitro cell culture conditions. Surprisingly, the pH and temperature conditions that mimicked B. burgdorferi residing in the gut of a fed tick showed a marked reduction in protein diversity. Additionally, the results provide us with leading candidates for exploring how B. burgdorferi adapts to and is able to survive in a wide variety of environmental conditions and lay a foundation for planned in situ studies of B. burgdorferi isolated from the tick midgut and infected animals.

  4. Hazardous Materials Incident Response Procedure

    Broader source: Energy.gov [DOE]

    The purpose of this procedure is to provide guidance for developing an emergency response plan, as outlined in OSHA’s 29 CFR 1910.120(q), for facility response.  This model has been adopted and...

  5. Departmental Radiological Emergency Response Assets

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-06-27

    The order establishes requirements and responsibilities for the DOE/NNSA national radiological emergency response assets and capabilities and Nuclear Emergency Support Team assets. Supersedes DOE O 5530.1A, DOE O 5530.2, DOE O 5530.3, DOE O 5530.4, and DOE O 5530.5.

  6. Demand Response Quick Assessment Tool

    Energy Science and Technology Software Center (OSTI)

    2008-12-01

    DRQAT (Demand Response Quick Assessment Tool) is the tool for assessing demand response saving potentials for large commercial buildings. This tool is based on EnergyPlus simulations of prototypical buildings and HVAC equipment. The opportunities for demand reduction and cost savings with building demand responsive controls vary tremendously with building type and location. The assessment tools will predict the energy and demand savings, the economic savings, and the thermal comfor impact for various demand responsive strategies.more » Users of the tools will be asked to enter the basic building information such as types, square footage, building envelope, orientation, utility schedule, etc. The assessment tools will then use the prototypical simulation models to calculate the energy and demand reduction potential under certain demand responsive strategies, such as precooling, zonal temperature set up, and chilled water loop and air loop set points adjustment.« less

  7. Automated Demand Response and Commissioning

    SciTech Connect (OSTI)

    Piette, Mary Ann; Watson, David S.; Motegi, Naoya; Bourassa, Norman

    2005-04-01

    This paper describes the results from the second season of research to develop and evaluate the performance of new Automated Demand Response (Auto-DR) hardware and software technology in large facilities. Demand Response (DR) is a set of activities to reduce or shift electricity use to improve the electric grid reliability and manage electricity costs. Fully-Automated Demand Response does not involve human intervention, but is initiated at a home, building, or facility through receipt of an external communications signal. We refer to this as Auto-DR. The evaluation of the control and communications must be properly configured and pass through a set of test stages: Readiness, Approval, Price Client/Price Server Communication, Internet Gateway/Internet Relay Communication, Control of Equipment, and DR Shed Effectiveness. New commissioning tests are needed for such systems to improve connecting demand responsive building systems to the electric grid demand response systems.

  8. Ethanol-induced cell damage in cultured rat antral mucosa assessed by chromium-51 release

    SciTech Connect (OSTI)

    Sewell, R.B.; Ling, T.S.; Yeomans, N.D.

    1986-08-01

    We have developed an in vitro method for studying ethanol-induced injury to gastric mucosa using organ culture of rat antrum. Cell damage was assessed by measurement of the release of (/sup 51/Cr)sodium chromate from preloaded cells, a method adapted from a standard immunologic technique. This system provided rapid and highly reproducible quantitation of tissue injury as assessed by /sup 51/Cr release into the culture medium. The threshold concentration for ethanol-induced damage was between 10 and 15% v/v, similar to in vivo thresholds observed by others. /sup 51/Cr release could also be induced by very short exposure to ethanol (5-15 min), and then continued despite ethanol removal. Interestingly, after continuous ethanol exposure, a plateau of maximum /sup 51/Cr release was reached 60 min after exposure to ethanol over the concentration range 20-50%, suggesting tissue adaptation to ethanol damage. This organ culture system, which allows precise control of experimental conditions, may be useful for studying mechanisms of gastric mucosal injury and protection.

  9. Adaptive controller for regenerative and friction braking system

    DOE Patents [OSTI]

    Davis, Roy I.

    1990-01-01

    A regenerative and friction braking system for a vehicle having one or more roadwheels driven by an electric traction motor includes a driver responsive device for producing a brake demand signal having a magnitude corresponding to the level of braking force selected by the driver and friction and regenerative brakes operatively connected with the roadwheels of the vehicle. A system according to this invention further includes control means for operating the friction and regenerative braking subsystems so that maximum brake torques sustainable by the roadwheels of the vehicle without skidding or slipping will not be exceeded.

  10. Adaptive controller for regenerative and friction braking system

    DOE Patents [OSTI]

    Davis, R.I.

    1990-10-16

    A regenerative and friction braking system for a vehicle having one or more road wheels driven by an electric traction motor includes a driver responsive device for producing a brake demand signal having a magnitude corresponding to the level of braking force selected by the driver and friction and regenerative brakes operatively connected with the road wheels of the vehicle. A system according to this invention further includes control means for operating the friction and regenerative braking subsystems so that maximum brake torques sustainable by the road wheels of the vehicle without skidding or slipping will not be exceeded. 8 figs.

  11. Honeywell Demonstrates Automated Demand Response Benefits for...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Honeywell Demonstrates Automated Demand Response Benefits for Utility, Commercial, and Industrial Customers Honeywell Demonstrates Automated Demand Response Benefits for Utility, ...

  12. Ultrafast Photovoltaic Response in Ferroelectric Nanolayers ...

    Office of Scientific and Technical Information (OSTI)

    Ultrafast Photovoltaic Response in Ferroelectric Nanolayers Citation Details In-Document Search Title: Ultrafast Photovoltaic Response in Ferroelectric Nanolayers Authors:...

  13. Wave-induced pore pressure and effective stresses in a porous seabed with variable permeability

    SciTech Connect (OSTI)

    Jeng, D.S.; Seymour, B.R.

    1996-12-31

    An evaluation of wave-induced soil response is particularly useful for geotechnical and coastal engineers involved in the design of foundations for offshore structures. To simplify the mathematical procedure, most theories available for the wave/seabed interaction problem have assumed a porous seabed with uniform permeability, despite strong evidence of variable permeability. This paper proposes an analytical solution for the wave induced soil response in a porous seabed with variable permeability. Verification is available through reduction to the simple case of uniform permeability. The numerical results indicate that the effect of variable soil permeability on pore pressure and effective stresses is significant.

  14. Adaptation of existing facilities to isentropic compression experiments

    SciTech Connect (OSTI)

    Tasker, Douglas G; Mielke, Charles H; Rodriguez, George; Rickel, Dwight G

    2011-01-07

    We demonstrate that the established pulsed power infrastructure at the National High Magnetic Field Laboratory - Pulsed Field Facility (NHMFL-PFF) at the Los Alamos National Laboratory can be adapted to obtain high quality isentropic compression experiment (ICE) data on materials in extreme conditions of dynamic high pressure. Experiments utilized a single-turn magnet pulsed power system at the NHMFL-PFF that was originally designed to measure actinide samples in extremes of high magnetic field (to 300 Tesla). A simple modification to the single-turn magnet has converted it to a fast turnaround dynamic high pressure measurement system. This paper details the work done including important background details that indicate that much more can be accomplished with optimization of the load characteristics in terms of ultimate peak pressures. To match the rise time of the NHMFL capacitor bank ({approx}2 {mu}s versus {approx}0.5 {mu}s for the Sandia Z-machine) the sample dimensions can be relatively large, i.e., up to 5 mm thickness. The maximum stresses are {approx}50GPa (0.5 Mbar) at the maximum bank voltage (60 kV) and higher pressures may be possible if the sample is tamped. For the design and predictions of performance of the NHMFL-ICE experiment it is important to have good predictive models. A SPICE code simulation was chosen to model all aspects of the experiment, electrical and physical. To this end, accurate dynamic load models were developed to simulate the compression and expansion of the dynamic load at high pressures using shock physics principles. A series experiments have been performed which demonstrated the feasibility of the NHMFL-ICE technique. The results will be shown and discussed. The NHMFL-ICE technique is an excellent method for measuring equations of state (EOS) at megabar pressures. Because a complete EOS can be obtained in one experiment from zero to the peak pressure, and because many shots can be fired in one day, the technique promises to provide high quality EOS data at relatively low cost.

  15. Detailed Modeling and Response of Demand Response Enabled Appliances

    SciTech Connect (OSTI)

    Vyakaranam, Bharat; Fuller, Jason C.

    2014-04-14

    Proper modeling of end use loads is very important in order to predict their behavior, and how they interact with the power system, including voltage and temperature dependencies, power system and load control functions, and the complex interactions that occur between devices in such an interconnected system. This paper develops multi-state time variant residential appliance models with demand response enabled capabilities in the GridLAB-DTM simulation environment. These models represent not only the baseline instantaneous power demand and energy consumption, but the control systems developed by GE Appliances to enable response to demand response signals and the change in behavior of the appliance in response to the signal. These DR enabled appliances are simulated to estimate their capability to reduce peak demand and energy consumption.

  16. Recent Results and Future Plans for a 45 Actuator Adaptive X...

    Office of Scientific and Technical Information (OSTI)

    X-ray Optics Experiment at the Advanced Light Source Citation Details In-Document Search Title: Recent Results and Future Plans for a 45 Actuator Adaptive X-ray Optics ...

  17. Recent Results and Future Plans for a 45 Actuator Adaptive X...

    Office of Scientific and Technical Information (OSTI)

    Optics Experiment at the Advanced Light Source Citation Details In-Document Search Title: Recent Results and Future Plans for a 45 Actuator Adaptive X-ray Optics Experiment at the ...

  18. An Enabling Study of Diesel Low-Temperautre Combustion via Adaptive Control

    Broader source: Energy.gov [DOE]

    Adaptive control strategies based on cylinder pressure and heat release characteristics are implemented to stablilize and enable lw temperature combustion from mid to high loads, at high boost and exhaust gas recirculation.

  19. Actuation fluid adapter for hydraulically-actuated electronically-controlled fuel injector and engine using same

    DOE Patents [OSTI]

    Keyster, Eric S.; Merchant, Jack A.

    2002-01-01

    A fuel injector adapter consists of a block defining a pressure communication passage therethrough and an actuation fluid passage. The actuation fluid passage includes three separate branches that open through an outer surface of the block at three separate locations.

  20. A hyper-spherical adaptive sparse-grid method for high-dimensional...

    Office of Scientific and Technical Information (OSTI)

    Title: A hyper-spherical adaptive sparse-grid method for high-dimensional discontinuity ... This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and ...

  1. The Effective Combination of Mesh Adaptation and Non-linearThermo...

    Office of Scientific and Technical Information (OSTI)

    The Effective Combination of Mesh Adaptation and Non-linear Thermo-mechanical Solution Components for the Modeling of Weld Failures. Citation Details In-Document Search Title: The...

  2. Climate Change and the Los Alamos National Laboratory: The Adaptation Challenge

    Broader source: Energy.gov [DOE]

    The Los Alamos National Laboratory (LANL) has been adapting to climate change related impacts that have been occurring on decadal time scales. The region where LANL is located has been subject to a...

  3. Climate Change Adaptation Technical Fact Sheet: Landfills and Containment as an Element of Site Remediation

    Broader source: Energy.gov [DOE]

    This fact sheet addresses contaminated site remedies involving source containment systems. It is intended to serve as an adaptation planning tool by (1) providing an overview of potential climate...

  4. An adaptive sparse-grid high-order stochastic collocation method for

    Office of Scientific and Technical Information (OSTI)

    Bayesian inference in groundwater reactive transport modeling (Technical Report) | SciTech Connect An adaptive sparse-grid high-order stochastic collocation method for Bayesian inference in groundwater reactive transport modeling Citation Details In-Document Search Title: An adaptive sparse-grid high-order stochastic collocation method for Bayesian inference in groundwater reactive transport modeling Although Bayesian analysis has become vital to the quantification of prediction uncertainty

  5. IMEX-a : an adaptive, fifth order implicit-explicit integration scheme.

    Office of Scientific and Technical Information (OSTI)

    (Technical Report) | SciTech Connect IMEX-a : an adaptive, fifth order implicit-explicit integration scheme. Citation Details In-Document Search Title: IMEX-a : an adaptive, fifth order implicit-explicit integration scheme. This report presents an efficient and accurate method for integrating a system of ordinary differential equations, particularly those arising from a spatial discretization of partially differential equations. The algorithm developed, termed the IMEX a algorithm, belongs

  6. Large-stroke MEMS deformable mirrors for adaptive optics. (Journal Article)

    Office of Scientific and Technical Information (OSTI)

    | SciTech Connect Journal Article: Large-stroke MEMS deformable mirrors for adaptive optics. Citation Details In-Document Search Title: Large-stroke MEMS deformable mirrors for adaptive optics. No abstract prepared. Authors: Spahn, Olga Blum ; Grossetete, Grant David ; Dagel, Daryl James ; Resnick, Paul James ; Shaw, Michael J. ; Grine, Alejandro J. ; Jokiel, Bernhard, Jr. [1] ; .) ; Cowan, William D. + Show Author Affiliations (, Publication Date: 2005-07-01 OSTI Identifier: 884737 Report

  7. Adapting On-Site Electrical Generation Platforms for Producer Gas - Fact

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sheet, April 2014 | Department of Energy Adapting On-Site Electrical Generation Platforms for Producer Gas - Fact Sheet, April 2014 Adapting On-Site Electrical Generation Platforms for Producer Gas - Fact Sheet, April 2014 The University of Minnesota, Morris, in collaboration with the University of Minnesota Center for Diesel Research, Cummins Power Generation Inc., ALL Power Labs, and Hammel, Green & Abrahamson (HGA), integrated a biomass gasifier and a reciprocating engine generator

  8. Adaptive coarse-graining for transient and quasi-equilibrium analyses of

    Office of Scientific and Technical Information (OSTI)

    stochastic gene regulation (Conference) | SciTech Connect Conference: Adaptive coarse-graining for transient and quasi-equilibrium analyses of stochastic gene regulation Citation Details In-Document Search Title: Adaptive coarse-graining for transient and quasi-equilibrium analyses of stochastic gene regulation Authors: Munsky, Brian [1] ; Tapia, Jose J [2] ; Faeder, James [2] + Show Author Affiliations Los Alamos National Laboratory UNIV OF PITTSBURGH Publication Date: 2012-04-04 OSTI

  9. Adaptive coarse-graining for transient and quasi-equilibrium analyses of

    Office of Scientific and Technical Information (OSTI)

    stochastic gene regulation (Conference) | SciTech Connect Conference: Adaptive coarse-graining for transient and quasi-equilibrium analyses of stochastic gene regulation Citation Details In-Document Search Title: Adaptive coarse-graining for transient and quasi-equilibrium analyses of stochastic gene regulation × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is

  10. An Adaptive Particle Filtering Approach to Tracking Modes in a Varying

    Office of Scientific and Technical Information (OSTI)

    Shallow Ocean Environment (Conference) | SciTech Connect An Adaptive Particle Filtering Approach to Tracking Modes in a Varying Shallow Ocean Environment Citation Details In-Document Search Title: An Adaptive Particle Filtering Approach to Tracking Modes in a Varying Shallow Ocean Environment The shallow ocean environment is ever changing mostly due to temperature variations in its upper layers (< 100m) directly affecting sound propagation throughout. The need to develop processors that

  11. An adaptive sparse-grid-based iterative ensemble Kalman filter approach for

    Office of Scientific and Technical Information (OSTI)

    parameter field estimation (Journal Article) | SciTech Connect An adaptive sparse-grid-based iterative ensemble Kalman filter approach for parameter field estimation Citation Details In-Document Search Title: An adaptive sparse-grid-based iterative ensemble Kalman filter approach for parameter field estimation Authors: Gunzburger, Max D [1] ; Webster, Clayton G [1] ; Zhang, Guannan [1] + Show Author Affiliations ORNL Publication Date: 2013-01-01 OSTI Identifier: 1096322 DOE Contract Number:

  12. Freedom of Information Act Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    You will have to assume risk of loss, or injury result from. your use of BPA's prop , -for such loss, or injury for which BPA may be responsible under the pmAom of the...

  13. Comment and Response Management System

    Energy Science and Technology Software Center (OSTI)

    1998-06-09

    CRMS is a Web-based client/server application that helps manage, track, and report on institutional responses to public comments on published documents such as environmental impact statements.

  14. Freedom of Information Act Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    information because it does not shed any light on how BPA has performed its statutory duties. Due to the size of the responsive documents they cannot be posted. To obtain a...

  15. Determination of surface recombination velocity at a grain boundary using electron-beam-induced current

    SciTech Connect (OSTI)

    Burk, D.E.; Kanner, S.; Muyshondt, J.E.; Shaulis, D.S.; Russell, P.E.

    1983-01-01

    In order to determine the surface recombination velocity at a grain boundary surface, computer-aided calculations of the theoretical electron-beam-induced-current response to a point source excitation are fitted to data taken as a function of distance from the grain boundary. It is demonstrated that the data is in good agreement with this theoretical response for distances greater than two maximum penetration depths of the incident electron beam.

  16. Intranuclear interactomic inhibition of NF-κB suppresses LPS-induced severe sepsis

    SciTech Connect (OSTI)

    Park, Sung-Dong; Cheon, So Yeong; Park, Tae-Yoon; Shin, Bo-Young; Oh, Hyunju; Ghosh, Sankar; Koo, Bon-Nyeo; Lee, Sang-Kyou

    2015-08-28

    Suppression of nuclear factor-κB (NF-κB) activation, which is best known as a major regulator of innate and adaptive immune responses, is a potent strategy for the treatment of endotoxic sepsis. To inhibit NF-κB functions, we designed the intra-nuclear transducible form of transcription modulation domain (TMD) of RelA (p65), called nt-p65-TMD, which can be delivered effectively into the nucleus without influencing the cell viability, and work as interactomic inhibitors via disruption of the endogenous p65-mediated transcription complex. nt-p65-TMD effectively inhibited the secretion of pro-inflammatory cytokines, including TNF-α, IL-1β, or IL-6 from BV2 microglia cells stimulated by lipopolysaccharide (LPS). nt-p65-TMD did not inhibit tyrosine phosphorylation of signaling mediators such as ZAP-70, p38, JNK, or ERK involved in T cell activation, but was capable of suppressing the transcriptional activity of NF-κB without the functional effect on that of NFAT upon T-cell receptor (TCR) stimulation. The transduced nt-p65-TMD in T cell did not affect the expression of CD69, however significantly inhibited the secretion of T cell-specific cytokines such as IL-2, IFN-γ, IL-4, IL-17A, or IL-10. Systemic administration of nt-p65-TMD showed a significant therapeutic effect on LPS-induced sepsis model by inhibiting pro-inflammatory cytokines secretion. Therefore, nt-p65-TMD can be a novel therapeutics for the treatment of various inflammatory diseases, including sepsis, where a transcription factor has a key role in pathogenesis, and further allows us to discover new functions of p65 under normal physiological condition without genetic alteration. - Highlights: • The nt-p65-TMD is intra-nuclear interactomic inhibitor of endogenous p65. • The nt-p65-TMD effectively inhibited the secretion of pro-inflammatory cytokines. • The excellent therapeutic potential of nt-p65-TMD was confirmed in sepsis model.

  17. Spinning Reserve From Hotel Load Response: Initial Progress

    SciTech Connect (OSTI)

    Kueck, John D; Kirby, Brendan J

    2008-11-01

    This project was motivated by the fundamental match between hotel space conditioning load response capability and power system contingency response needs. As power system costs rise and capacity is strained demand response can provide a significant system reliability benefit at a potentially attractive cost. At ORNL s suggestion, Digital Solutions Inc. adapted its hotel air conditioning control technology to supply power system spinning reserve. This energy saving technology is primarily designed to provide the hotel operator with the ability to control individual room temperature set-points based upon occupancy (25% to 50% energy savings based on an earlier study [Kirby and Ally, 2002]). DSI added instantaneous local load shedding capability in response to power system frequency and centrally dispatched load shedding capability in response to power system operator command. The 162 room Music Road Hotel in Pigeon Forge Tennessee agreed to host the spinning reserve test. The Tennessee Valley Authority supplied real-time metering equipment in the form of an internet connected Dranetz-BMI power quality meter and monitoring expertise to record total hotel load during both normal operations and test results. The Sevier County Electric System installed the metering. Preliminary testing showed that hotel load can be curtailed by 22% to 37% depending on the outdoor temperature and the time of day. These results are prior to implementing control over the common area air conditioning loads. Testing was also not at times of highest system or hotel loading. Full response occurred in 12 to 60 seconds from when the system operator s command to shed load was issued. The load drop was very rapid, essentially as fast as the 2 second metering could detect, with all units responding essentially simultaneously. Load restoration was ramped back in over several minutes. The restoration ramp can be adjusted to the power system needs. Frequency response testing was not completed. Initial testing showed that the units respond very quickly. Problems with local power quality generated false low frequency signals which required testing to be stopped. This should not be a problem in actual operation since the frequency trip points will be staggered to generate a droop curve which mimics generator governor response. The actual trip frequencies will also be low enough to avoid power quality problems. The actual trip frequencies are too low to generate test events with sufficient regularity to complete testing in a reasonable amount of time. Frequency response testing will resume once the local power quality problem is fully understood and reasonable test frequency settings can be determined. Overall the preliminary testing was extremely successful. The hotel response capability matches the power system reliability need, being faster than generation response and inherently available when the power system is under the most stress (times of high system and hotel load). Periodic testing is scheduled throughout the winter and spring to characterize hotel response capability under a full range of conditions. More extensive testing will resume when summer outdoor temperatures are again high enough to fully test hotel response.

  18. Role of reactive nitrogen species generated via inducible nitric oxide synthase in vesicant-induced lung injury, inflammation and altered lung functioning

    SciTech Connect (OSTI)

    Sunil, Vasanthi R.; Shen, Jianliang; Patel-Vayas, Kinal; Gow, Andrew J.; Laskin, Jeffrey D.; Laskin, Debra L.

    2012-05-15

    Pulmonary toxicity induced by sulfur mustard and related vesicants is associated with oxidative stress. In the present studies we analyzed the role of reactive nitrogen species (RNS) generated via inducible nitric oxide synthase (iNOS) in lung injury and inflammation induced by vesicants using 2-chloroethyl ethyl sulfide (CEES) as a model. C57Bl/6 (WT) and iNOS ?/? mice were sacrificed 3 days or 14 days following intratracheal administration of CEES (6 mg/kg) or control. CEES intoxication resulted in transient (3 days) increases in bronchoalveolar lavage (BAL) cell and protein content in WT, but not iNOS ?/? mice. This correlated with expression of Ym1, a marker of oxidative stress in alveolar macrophages and epithelial cells. In contrast, in iNOS ?/? mice, Ym1 was only observed 14 days post-exposure in enlarged alveolar macrophages, suggesting that they are alternatively activated. This is supported by findings that lung tumor necrosis factor and lipocalin Lcn2 expression, mediators involved in tissue repair were also upregulated at this time in iNOS ?/? mice. Conversely, CEES-induced increases in the proinflammatory genes, monocyte chemotactic protein-1 and cyclooxygenase-2, were abrogated in iNOS ?/? mice. In WT mice, CEES treatment also resulted in increases in total lung resistance and decreases in compliance in response to methacholine, effects blunted by loss of iNOS. These data demonstrate that RNS, generated via iNOS play a role in the pathogenic responses to CEES, augmenting oxidative stress and inflammation and suppressing tissue repair. Elucidating inflammatory mechanisms mediating vesicant-induced lung injury is key to the development of therapeutics to treat mustard poisoning. -- Highlights: ? Lung injury, inflammation and oxidative stress are induced by the model vesicant CEES ? RNS generated via iNOS are important in the CEES-induced pulmonary toxicity ? iNOS ?/? mice are protected from CEES-induced lung toxicity and altered lung functioning.

  19. Opportunities for Energy Efficiency and Automated Demand Response in Industrial Refrigerated Warehouses in California

    SciTech Connect (OSTI)

    Lekov, Alex; Thompson, Lisa; McKane, Aimee; Rockoff, Alexandra; Piette, Mary Ann

    2009-05-11

    This report summarizes the Lawrence Berkeley National Laboratory's research to date in characterizing energy efficiency and open automated demand response opportunities for industrial refrigerated warehouses in California. The report describes refrigerated warehouses characteristics, energy use and demand, and control systems. It also discusses energy efficiency and open automated demand response opportunities and provides analysis results from three demand response studies. In addition, several energy efficiency, load management, and demand response case studies are provided for refrigerated warehouses. This study shows that refrigerated warehouses can be excellent candidates for open automated demand response and that facilities which have implemented energy efficiency measures and have centralized control systems are well-suited to shift or shed electrical loads in response to financial incentives, utility bill savings, and/or opportunities to enhance reliability of service. Control technologies installed for energy efficiency and load management purposes can often be adapted for open automated demand response (OpenADR) at little additional cost. These improved controls may prepare facilities to be more receptive to OpenADR due to both increased confidence in the opportunities for controlling energy cost/use and access to the real-time data.

  20. A case for Sandia investment in complex adaptive systems science and technology.

    SciTech Connect (OSTI)

    Colbaugh, Richard; Tsao, Jeffrey Yeenien; Johnson, Curtis Martin; Backus, George A.; Brown, Theresa Jean; Jones, Katherine A.

    2012-05-01

    This white paper makes a case for Sandia National Laboratories investments in complex adaptive systems science and technology (S&T) -- investments that could enable higher-value-added and more-robustly-engineered solutions to challenges of importance to Sandia's national security mission and to the nation. Complex adaptive systems are ubiquitous in Sandia's national security mission areas. We often ignore the adaptive complexity of these systems by narrowing our 'aperture of concern' to systems or subsystems with a limited range of function exposed to a limited range of environments over limited periods of time. But by widening our aperture of concern we could increase our impact considerably. To do so, the science and technology of complex adaptive systems must mature considerably. Despite an explosion of interest outside of Sandia, however, that science and technology is still in its youth. What has been missing is contact with real (rather than model) systems and real domain-area detail. With its center-of-gravity as an engineering laboratory, Sandia's has made considerable progress applying existing science and technology to real complex adaptive systems. It has focused much less, however, on advancing the science and technology itself. But its close contact with real systems and real domain-area detail represents a powerful strength with which to help complex adaptive systems science and technology mature. Sandia is thus both a prime beneficiary of, as well as potentially a prime contributor to, complex adaptive systems science and technology. Building a productive program in complex adaptive systems science and technology at Sandia will not be trivial, but a credible path can be envisioned: in the short run, continue to apply existing science and technology to real domain-area complex adaptive systems; in the medium run, jump-start the creation of new science and technology capability through Sandia's Laboratory Directed Research and Development program; and in the long run, inculcate an awareness at the Department of Energy of the importance of supporting complex adaptive systems science through its Office of Science.

  1. Chemical chaperones reduce ionizing radiation-induced endoplasmic reticulum stress and cell death in IEC-6 cells

    SciTech Connect (OSTI)

    Lee, Eun Sang; Lee, Hae-June; Lee, Yoon-Jin; Jeong, Jae-Hoon; Kang, Seongman; Lim, Young-Bin

    2014-07-25

    Highlights: UPR activation precedes caspase activation in irradiated IEC-6 cells. Chemical ER stress inducers radiosensitize IEC-6 cells. siRNAs that targeted ER stress responses ameliorate IR-induced cell death. Chemical chaperons prevent cell death in irradiated IEC-6 cells. - Abstract: Radiotherapy, which is one of the most effective approaches to the treatment of various cancers, plays an important role in malignant cell eradication in the pelvic area and abdomen. However, it also generates some degree of intestinal injury. Apoptosis in the intestinal epithelium is the primary pathological factor that initiates radiation-induced intestinal injury, but the mechanism by which ionizing radiation (IR) induces apoptosis in the intestinal epithelium is not clearly understood. Recently, IR has been shown to induce endoplasmic reticulum (ER) stress, thereby activating the unfolded protein response (UPR) signaling pathway in intestinal epithelial cells. However, the consequences of the IR-induced activation of the UPR signaling pathway on radiosensitivity in intestinal epithelial cells remain to be determined. In this study, we investigated the role of ER stress responses in IR-induced intestinal epithelial cell death. We show that chemical ER stress inducers, such as tunicamycin or thapsigargin, enhanced IR-induced caspase 3 activation and DNA fragmentation in intestinal epithelial cells. Knockdown of Xbp1 or Atf6 with small interfering RNA inhibited IR-induced caspase 3 activation. Treatment with chemical chaperones prevented ER stress and subsequent apoptosis in IR-exposed intestinal epithelial cells. Our results suggest a pro-apoptotic role of ER stress in IR-exposed intestinal epithelial cells. Furthermore, inhibiting ER stress may be an effective strategy to prevent IR-induced intestinal injury.

  2. Mind the gap in SEA: An institutional perspective on why assessment of synergies amongst climate change mitigation, adaptation and other policy areas are missing

    SciTech Connect (OSTI)

    Vammen Larsen, Sanne; Kornov, Lone; Wejs, Anja

    2012-02-15

    This article takes its point of departure in two approaches to integrating climate change into Strategic Environmental Assessment (SEA): Mitigation and adaptation, and in the fact that these, as well as the synergies between them and other policy areas, are needed as part of an integrated assessment and policy response. First, the article makes a review of how positive and negative synergies between a) climate change mitigation and adaptation and b) climate change and other environmental concerns are integrated into Danish SEA practice. Then, the article discusses the implications of not addressing synergies. Finally, the article explores institutional explanations as to why synergies are not addressed in SEA practice. A document analysis of 149 Danish SEA reports shows that only one report comprises the assessment of synergies between mitigation and adaptation, whilst 9,4% of the reports assess the synergies between climate change and other environmental concerns. The consequences of separation are both the risk of trade-offs and missed opportunities for enhancing positive synergies. In order to propose explanations for the lacking integration, the institutional background is analysed and discussed, mainly based on Scott's theory of institutions. The institutional analysis highlights a regulatory element, since the assessment of climate change synergies is underpinned by legislation, but not by guidance. This means that great focus is on normative elements such as the local interpretation of legislation and of climate change mitigation and adaptation. The analysis also focuses on how the fragmentation of the organisation in which climate change and SEA are embedded has bearings on both normative and cultural-cognitive elements. This makes the assessment of synergies challenging. The evidence gathered and presented in the article points to a need for developing the SEA process and methodology in Denmark with the aim to include climate change in the assessments in a more systematic and integrated manner. - Highlights: Black-Right-Pointing-Pointer Synergies between climate change mitigation, adaptation and other environmental concerns are not addressed in Danish SEA. Black-Right-Pointing-Pointer Institutional explanations relate to organisational set-ups and understandings of climate change as a new planning issue. Black-Right-Pointing-Pointer The paper points to a need for developing SEA to include climate change in a more systematic and integrated manner.

  3. Oxidative stress-induced autophagy: Role in pulmonary toxicity

    SciTech Connect (OSTI)

    Malaviya, Rama; Laskin, Jeffrey D.; Laskin, Debra L.

    2014-03-01

    Autophagy is an evolutionarily conserved catabolic process important in regulating the turnover of essential proteins and in elimination of damaged organelles and protein aggregates. Autophagy is observed in the lung in response to oxidative stress generated as a consequence of exposure to environmental toxicants. Whether autophagy plays role in promoting cell survival or cytotoxicity is unclear. In this article recent findings on oxidative stress-induced autophagy in the lung are reviewed; potential mechanisms initiating autophagy are also discussed. A better understanding of autophagy and its role in pulmonary toxicity may lead to the development of new strategies to treat lung injury associated with oxidative stress. - Highlights: Exposure to pulmonary toxicants is associated with oxidative stress. Oxidative stress is known to induce autophagy. Autophagy is upregulated in the lung following exposure to pulmonary toxicants. Autophagy may be protective or pathogenic.

  4. Response

    Energy Savers [EERE]

    Energy Introduction Over the past seven years, the Department of Energy (DOE) has ... Typical awards are 22-25 millionyear for up to five years. * Advanced Research Projects ...

  5. Response

    Energy Savers [EERE]

    Responding To Hurricane Sandy: DOE Situation Reports Responding To Hurricane Sandy: DOE Situation Reports November 7, 2012 - 11:15am Addthis Sandy made landfall as a post-tropical cyclone on the southern coast of New Jersey near Atlantic City at 8 p.m. on October 29, with top sustained winds of 80 mph. | Photo courtesy of NOAA. Sandy made landfall as a post-tropical cyclone on the southern coast of New Jersey near Atlantic City at 8 p.m. on October 29, with top sustained winds of 80 mph. | Photo

  6. Responsibility

    Energy Savers [EERE]

    involved. Can we have our cake and eat it too? RE THINK NEW STORE 9 Challenges and Lessons Being Learned 1. Project Evaluation and Approval a) Communicating the total value...

  7. ADAPTATION OF CRACK GROWTH DETECTION TECHNIQUES TO US MATERIAL TEST REACTORS

    SciTech Connect (OSTI)

    A. Joseph Palmer; Sebastien P. Teysseyre; Kurt L. Davis; Gordon Kohse; Yakov Ostrovsky; David M. Carpenter; Joy L. Rempe

    2015-04-01

    A key component in evaluating the ability of Light Water Reactors to operate beyond 60 years is characterizing the degradation of materials exposed to radiation and various water chemistries. Of particular concern is the response of reactor materials to Irradiation Assisted Stress Corrosion Cracking (IASCC). Some test reactors outside the United States, such as the Halden Boiling Water Reactor (HBWR), have developed techniques to measure crack growth propagation during irradiation. The basic approach is to use a custom-designed compact loading mechanism to stress the specimen during irradiation, while the crack in the specimen is monitored in-situ using the Direct Current Potential Drop (DCPD) method. In 2012 the US Department of Energy commissioned the Idaho National Laboratory and the MIT Nuclear Reactor Laboratory (MIT NRL) to take the basic concepts developed at the HBWR and adapt them to a test rig capable of conducting in-pile IASCC tests in US Material Test Reactors. The first two and half years of the project consisted of designing and testing the loader mechanism, testing individual components of the in-pile rig and electronic support equipment, and autoclave testing of the rig design prior to insertion in the MIT Reactor. The load was applied to the specimen by means of a scissor like mechanism, actuated by a miniature metal bellows driven by pneumatic pressure and sized to fit within the small in-core irradiation volume. In addition to the loader design, technical challenges included developing robust connections to the specimen for the applied current and voltage measurements, appropriate ceramic insulating materials that can endure the LWR environment, dealing with the high electromagnetic noise environment of a reactor core at full power, and accommodating material property changes in the specimen, due primarily to fast neutron damage, which change the specimen resistance without additional crack growth. The project culminated with an in-pile demonstration at the MIT Reactor. The test rig and associated support equipment were used to apply loads to a representative Compact Tensile specimen during one MITR operating cycle, while measuring crack growth using the DCPD method. Although the test period was short (approximately 70 days), and the accumulated neutron dose relatively small, successful operation of the test rig was demonstrated. The specimen was cycled more than 8000 times (more than would be typical for a long term IASCC test), which was sufficient to propagate a crack of over 2 mm.

  8. Dynamic frequency tuning of electric and magnetic metamaterial response

    DOE Patents [OSTI]

    O'Hara, John F; Averitt, Richard; Padilla, Willie; Chen, Hou-Tong

    2014-09-16

    A geometrically modifiable resonator is comprised of a resonator disposed on a substrate, and a means for geometrically modifying the resonator. The geometrically modifiable resonator can achieve active optical and/or electronic control of the frequency response in metamaterials and/or frequency selective surfaces, potentially with sub-picosecond response times. Additionally, the methods taught here can be applied to discrete geometrically modifiable circuit components such as inductors and capacitors. Principally, controlled conductivity regions, using either reversible photodoping or voltage induced depletion activation, are used to modify the geometries of circuit components, thus allowing frequency tuning of resonators without otherwise affecting the bulk substrate electrical properties. The concept is valid over any frequency range in which metamaterials are designed to operate.

  9. Adapting Wireless Technology to Lighting Control and Environmental Sensing

    SciTech Connect (OSTI)

    Dana Teasdale; Francis Rubinstein; Dave Watson; Steve Purdy

    2005-10-01

    The high cost of retrofitting buildings with advanced lighting control systems is a barrier to adoption of this energy-saving technology. Wireless technology, however, offers a solution to mounting installation costs since it requires no additional wiring to implement. To demonstrate the feasibility of such a system, a prototype wirelessly-controlled advanced lighting system was designed and built. The system includes the following components: a wirelessly-controllable analog circuit module (ACM), a wirelessly-controllable electronic dimmable ballast, a T8 3-lamp fixture, an environmental multi-sensor, a current transducer, and control software. The ACM, dimmable ballast, multi-sensor, and current transducer were all integrated with SmartMesh{trademark} wireless mesh networking nodes, called motes, enabling wireless communication, sensor monitoring, and actuator control. Each mote-enabled device has a reliable communication path to the SmartMesh Manager, a single board computer that controls network functions and connects the wireless network to a PC running lighting control software. The ACM is capable of locally driving one or more standard 0-10 Volt electronic dimmable ballasts through relay control and a 0-10 Volt controllable output. The mote-integrated electronic dimmable ballast is designed to drive a standard 3-lamp T8 light fixture. The environmental multi-sensor measures occupancy, light level and temperature. The current transducer is used to measure the power consumed by the fixture. Control software was developed to implement advanced lighting algorithms, including daylight ramping, occupancy control, and demand response. Engineering prototypes of each component were fabricated and tested in a bench-scale system. Based on standard industry practices, a cost analysis was conducted. It is estimated that the installation cost of a wireless advanced lighting control system for a retrofit application is at least 30% lower than a comparable wired system for a typical 16,000 square-foot office building, with a payback period of less than 3 years.

  10. Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials...

    Office of Scientific and Technical Information (OSTI)

    C.; Pop, Eric; Wong, H.-S.Philip; Wuttig, Matthias; Lindenberg, Aaron M. MATSCI, OPTICS, PHYS MATSCI, OPTICS, PHYS Abstract Not Provided http:www-public.slac.stanford.edu...

  11. Ultrafast Terahertz-Induced Response of GeSbTe Phase-Change Materials...

    Office of Scientific and Technical Information (OSTI)

    Close Cite: Bibtex Format Close 0 pages in this document matching the terms "" Search For Terms: Enter terms in the toolbar above to search the full text of this document for ...

  12. Fuel-Induced System Responses The Role Unconventional Fuels May Play in

    Broader source: Energy.gov (indexed) [DOE]

    Model | Department of Energy presentation by Michael Wang of Argonne National Laboratory provides information about an analysis of hydrogen-powered fuel-cell systems. PDF icon fuel_cycle_comparison_forklifts_presentation.pdf More Documents & Publications Fuel Cell Comparison of Distributed Power Generation Technologies Fuel-Cycle Energy and Emissions Analysis with the GREET Model Full Fuel-Cycle Comparison of Forklift Propulsion Systems of Energy

    09 DOE Hydrogen Program and

  13. Photo-induced micro-mechanical optical switch

    DOE Patents [OSTI]

    Rajic, Slobodan; Datskos, Panagiotis George; Egert, Charles M.

    2002-01-01

    An optical switch is formed by introducing light lengthwise to a microcantilever waveguide directed toward a second waveguide. The microcantilever is caused to bend by light emitted from a laser diode orthogonal to the microcantilever and at an energy above the band gap, which induces stress as a result of the generation of free carriers. The bending of the waveguide directs the carrier frequency light to a second receptor waveguide or to a non-responsive surface. The switch may be combined in an array to perform multiple switching functions rapidly and at low energy losses.

  14. PEGylation of a Maltose Biosensor Promotes Enhanced Signal Response

    SciTech Connect (OSTI)

    Dattelbaum, Andrew; Baker, Gary A; Fox, John M; Iyer, Srinivas; Dattelbaum, Jonathan

    2009-01-01

    A robust method to immobilize a maltose biosensor is described using an engineered maltose periplasmic binding protein (PBP) covalently coupled to NBDamide, an environmentally sensitive fluorophore. A mesoporous silica sol-gel derived from diglycerylsilane (DGS) was constructed to embed the maltose biosensor, and the ligand reporting fluorescence properties were meas red. When sequestered in the DGS-derived silica matrix, the biosensor retained maltose-dependent fluorescence sensing capability with micromolar affinity, which is consistent with the protein free in solution. The MBP-NBD conjugate was further modified by covalent conjugation with poly(ethylene glycol)-5000 (PEG) to promote the retention of water molecules around the protein and to reduce possible steric effects between the silica matrix and protein. Bioconjugation with PEG molecules does not significantly affect the signaling response of the protein in solution. When immobilized in the DGS polymer, a consistent increase in fluorescence intensity was observed as compared to the protein not functionalized with PEG. To our knowledge, this report presents the first successful method to embed a PBP biosensor in a polymerized matrix and retain signaling response using an environmentally sensitive probe. The immobilization method presented here should be easily adaptable to all conformation-dependent biosensors.

  15. Recovery Act: Energy Efficiency of Data Networks through Rate Adaptation (EEDNRA) - Final Technical Report

    SciTech Connect (OSTI)

    Matthew Andrews; Spyridon Antonakopoulos; Steve Fortune; Andrea Francini; Lisa Zhang

    2011-07-12

    This Concept Definition Study focused on developing a scientific understanding of methods to reduce energy consumption in data networks using rate adaptation. Rate adaptation is a collection of techniques that reduce energy consumption when traffic is light, and only require full energy when traffic is at full provisioned capacity. Rate adaptation is a very promising technique for saving energy: modern data networks are typically operated at average rates well below capacity, but network equipment has not yet been designed to incorporate rate adaptation. The Study concerns packet-switching equipment, routers and switches; such equipment forms the backbone of the modern Internet. The focus of the study is on algorithms and protocols that can be implemented in software or firmware to exploit hardware power-control mechanisms. Hardware power-control mechanisms are widely used in the computer industry, and are beginning to be available for networking equipment as well. Network equipment has different performance requirements than computer equipment because of the very fast rate of packet arrival; hence novel power-control algorithms are required for networking. This study resulted in five published papers, one internal report, and two patent applications, documented below. The specific technical accomplishments are the following: A model for the power consumption of switching equipment used in service-provider telecommunication networks as a function of operating state, and measured power-consumption values for typical current equipment. An algorithm for use in a router that adapts packet processing rate and hence power consumption to traffic load while maintaining performance guarantees on delay and throughput. An algorithm that performs network-wide traffic routing with the objective of minimizing energy consumption, assuming that routers have less-than-ideal rate adaptivity. An estimate of the potential energy savings in service-provider networks using feasibly-implementable rate adaptivity. A buffer-management algorithm that is designed to reduce the size of router buffers, and hence energy consumed. A packet-scheduling algorithm designed to minimize packet-processing energy requirements. Additional research is recommended in at least two areas: further exploration of rate-adaptation in network switching equipment, including incorporation of rate-adaptation in actual hardware, allowing experimentation in operational networks; and development of control protocols that allow parts of networks to be shut down while minimizing disruption to traffic flow in the network. The research is an integral part of a large effort within Bell Laboratories, Alcatel-Lucent, aimed at dramatic improvements in the energy efficiency of telecommunication networks. This Study did not explicitly consider any commercialization opportunities.

  16. ATVM Response to MEMA | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ATVM Response to MEMA ATVM Response to MEMA PDF icon ATVM Response to MEMA.pdf More Documents & Publications Interested Parties - MEMA MEMA: Letter MEMA: Appendix to Comments

  17. International Conference on Climate Change Adaptation Assessments: Conference summary and statement

    SciTech Connect (OSTI)

    1995-08-01

    The International Conference on Climate Change Adaptation Assessments was held in St. Petersburg, Russian Federation, from May 22--25, 1995. Sponsored by the Russian Federal Service for Hydrometeorology and Environmental Monitoring, the US Country Studies Program, and the directorate General for International Cooperation of the Netherlands Government, it was the first international conference focusing exclusively on adaptation to climate change. More than 100 people from 29 countries on five continents participated. The conference primarily addressed measures to anticipate the potential effects of climate change to minimize negative effects and take advantage of any positive effects. The focus was on what governments, institutions, and individuals can do to prepare for climate change. The conference dealt with two major topics: What adaptation options are most effective and efficient in anticipating climate change and what methods should be used to assess the effectiveness and efficiency of adaptation options. Brief summaries are given from the following sessions on agriculture; Water resources; coastal resources; ecosystems and forests; fisheries; human settlements; water and agriculture; and the panel session on international adaptation in national communications and other development plans and needs for technical assistance.

  18. Markets during world oil supply crises: an analysis of industry, consumer, and governmental response

    SciTech Connect (OSTI)

    Erfle, Stephen; Pound, John; Kalt, Joseph

    1981-04-01

    An analysis of the response of American markets to supply crises in world oil markets is presented. It addresses four main issues: the efficiency of the operation of American oil markets during oil supply crises; the problems of both economic efficiency and social equity which arise during the American adaptation process; the propriety of the Federal government's past policy responses to these problems; and the relationship between perceptions of the problems caused by world oil crises and the real economic natures of these problems. Specifically, Chapter 1 presents a theoretical discussion of the effects of a world supply disruption on the price level and supply availability of the world market oil to any consuming country including the US Chapter 2 provides a theoretical and empirical analysis of the efficiency of the adaptations of US oil product markets to higher world oil prices. Chapter 3 examines the responses of various groups of US oil firms to the alterations observed in world markets, while Chapter 4 presents a theoretical explanation for the price-lagging behavior exhibited by firms in the US oil industry. Chapter 5 addresses the nature of both real and imagined oil market problems in the US during periods of world oil market transition. (MCW)

  19. Addressing Energy Demand through Demand Response. International Experiences and Practices

    SciTech Connect (OSTI)

    Shen, Bo; Ghatikar, Girish; Ni, Chun Chun; Dudley, Junqiao; Martin, Phil; Wikler, Greg

    2012-06-01

    Demand response (DR) is a load management tool which provides a cost-effective alternative to traditional supply-side solutions to address the growing demand during times of peak electrical load. According to the US Department of Energy (DOE), demand response reflects “changes in electric usage by end-use customers from their normal consumption patterns in response to changes in the price of electricity over time, or to incentive payments designed to induce lower electricity use at times of high wholesale market prices or when system reliability is jeopardized.” 1 The California Energy Commission (CEC) defines DR as “a reduction in customers’ electricity consumption over a given time interval relative to what would otherwise occur in response to a price signal, other financial incentives, or a reliability signal.” 2 This latter definition is perhaps most reflective of how DR is understood and implemented today in countries such as the US, Canada, and Australia where DR is primarily a dispatchable resource responding to signals from utilities, grid operators, and/or load aggregators (or DR providers).

  20. Demand Response in the ERCOT Markets

    SciTech Connect (OSTI)

    Patterson, Mark

    2011-10-25

    ERCOT grid serves 85% of Texas load over 40K+ miles transmission line. Demand response: voluntary load response, load resources, controllable load resources, and emergency interruptible load service.

  1. Accident Response Group | National Nuclear Security Administration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Accident Response Group NNSA's Accident Response Group (ARG) provides technical guidance and responds to U.S. nuclear weapons accidents. ARGLogo The team assists in assessing ...

  2. emergency response assets | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    Accident Response Group NNSA's Accident Response Group (ARG) provides technical guidance and responds to U.S. nuclear weapons accidents. The team assists in assessing weapons ...

  3. Section 2.2 (Roles and Responsibilities)

    Broader source: Energy.gov [DOE]

    Describes responsibilities of program managers, office directors, and the peer review leader, as well as corporate responsibilities, for conducting peer reviews.

  4. HIV vaccine strategy expands immune responses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    HIV vaccine strategy expands immune responses HIV vaccine strategy expands immune responses Two teams of researchers have announced an HIV vaccination strategy that has been shown...

  5. National Electrical Manufacturers Association (NEMA) Response...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electrical Manufacturers Association (NEMA) Response to Smart Grid RFI National Electrical Manufacturers Association (NEMA) Response to Smart Grid RFI The National Electrical ...

  6. BPA-2011-00384-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    damaged due to gunfire, on or around January 2010, in the Tiger Mountain Summit area of King County Washington. Response: BPA has provided a responsive document with the...

  7. BPA-2012-01842-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to the transfer of land at Craig Mountain from BPA to Idaho Department of Fish and Game. Response: BPA's Congressional Correspondence Office has no records responsive to your...

  8. BPA-2014-01685-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    no responsive records (the winning proposal). There has been no contract awarded for the Demand Response Program Demonstration Project RFP dated May 23,2014. This notification...

  9. BPA-2014-01901-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2014, and the agreement between Bonneville Power Administration and Energy Northwest for Demand Response Pilot announced in September 2014." Response We conducted a search ofthe...

  10. Demand Response - Policy | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Demand Response - Policy Demand Response - Policy Since its inception, the Office of Electricity Delivery and Energy Reliability (OE) has been committed to modernizing the nation's ...

  11. BPA-2013-01310-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (OPM) or DOE Human Capital, or any inquiries, reviews and investigations into BPA hiring and promotion practices." Response We have located 471 pages of material responsive...

  12. bpa-2013-01407-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    or DOE Human Capital, or any inquiries, reviews and investigations into BPA hiring and promotion practices." Response: We have located 26 pages of material responsive to your...

  13. Program Support From Socially Responsible Investing | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Support From Socially Responsible Investing Better Buildings Residential Financing and Revenue Peer Exchange Call Series: Socially Responsible Investing, Call Slides and Discussion...

  14. radiological response | National Nuclear Security Administration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Apply for Our Jobs Our Jobs Working at NNSA Blog Home radiological response radiological response Fukushima: Five Years Later After the March 11, 2011, Japan earthquake, tsunami, ...

  15. BPA-2013-00334-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    but not limited to, the use of existing access roads and tower locations on wetlands. Response: BPA has no documents responsive to your request. Pursuant to 10 CFR...

  16. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    induced incandescence (LII) - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs

  17. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Davis, Ryan W.; Carvalho, Benjamin J.; Jones, Howland D. T.; Singh, Seema

    2014-05-13

    Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopicmore » and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.« less

  18. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

    SciTech Connect (OSTI)

    Davis, Ryan W.; Carvalho, Benjamin J.; Jones, Howland D. T.; Singh, Seema

    2014-05-13

    Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.

  19. Adaptive nonlocal means filtering based on local noise level for CT denoising

    SciTech Connect (OSTI)

    Li, Zhoubo; Trzasko, Joshua D.; Lake, David S.; Blezek, Daniel J.; Manduca, Armando; Yu, Lifeng; Fletcher, Joel G.; McCollough, Cynthia H.

    2014-01-15

    Purpose: To develop and evaluate an image-domain noise reduction method based on a modified nonlocal means (NLM) algorithm that is adaptive to local noise level of CT images and to implement this method in a time frame consistent with clinical workflow. Methods: A computationally efficient technique for local noise estimation directly from CT images was developed. A forward projection, based on a 2D fan-beam approximation, was used to generate the projection data, with a noise model incorporating the effects of the bowtie filter and automatic exposure control. The noise propagation from projection data to images was analytically derived. The analytical noise map was validated using repeated scans of a phantom. A 3D NLM denoising algorithm was modified to adapt its denoising strength locally based on this noise map. The performance of this adaptive NLM filter was evaluated in phantom studies in terms of in-plane and cross-plane high-contrast spatial resolution, noise power spectrum (NPS), subjective low-contrast spatial resolution using the American College of Radiology (ACR) accreditation phantom, and objective low-contrast spatial resolution using a channelized Hotelling model observer (CHO). Graphical processing units (GPU) implementation of this noise map calculation and the adaptive NLM filtering were developed to meet demands of clinical workflow. Adaptive NLM was piloted on lower dose scans in clinical practice. Results: The local noise level estimation matches the noise distribution determined from multiple repetitive scans of a phantom, demonstrated by small variations in the ratio map between the analytical noise map and the one calculated from repeated scans. The phantom studies demonstrated that the adaptive NLM filter can reduce noise substantially without degrading the high-contrast spatial resolution, as illustrated by modulation transfer function and slice sensitivity profile results. The NPS results show that adaptive NLM denoising preserves the shape and peak frequency of the noise power spectrum better than commercial smoothing kernels, and indicate that the spatial resolution at low contrast levels is not significantly degraded. Both the subjective evaluation using the ACR phantom and the objective evaluation on a low-contrast detection task using a CHO model observer demonstrate an improvement on low-contrast performance. The GPU implementation can process and transfer 300 slice images within 5 min. On patient data, the adaptive NLM algorithm provides more effective denoising of CT data throughout a volume than standard NLM, and may allow significant lowering of radiation dose. After a two week pilot study of lower dose CT urography and CT enterography exams, both GI and GU radiology groups elected to proceed with permanent implementation of adaptive NLM in their GI and GU CT practices. Conclusions: This work describes and validates a computationally efficient technique for noise map estimation directly from CT images, and an adaptive NLM filtering based on this noise map, on phantom and patient data. Both the noise map calculation and the adaptive NLM filtering can be performed in times that allow integration with clinical workflow. The adaptive NLM algorithm provides effective denoising of CT data throughout a volume, and may allow significant lowering of radiation dose.

  20. Implications of simultaneously mitigating and adapting to climate change: Initial experiments using GCAM

    SciTech Connect (OSTI)

    Calvin, Katherine V.; Wise, Marshall A.; Clarke, Leon E.; Edmonds, James A.; Kyle, G. Page; Luckow, Patrick W.; Thomson, Allison M.

    2013-04-01

    Historically climate impacts research and climate mitigation research have been two separate and independent domains of inquiry. Climate mitigation research has investigated greenhouse gas emissions assuming that climate is unchanging. At the same time climate mitigation research has investigated the implications of climate change on the assumption that climate mitigation will proceed without affecting the degree of climate impacts or the ability of human and natural systems to adapt. The Global Change Assessment Model (GCAM) has largely been employed to study climate mitigation. Here we explore the development of capabilities to assess climate change impacts and adaptation within the GCAM model. These capabilities are being developed so as to be able to simultaneously reconcile the joint implications of climate change mitigation, impacts and adaptive potential. This is an important step forward in that it enables direct comparison between climate mitigation activities and climate impacts and the opportunity to understand interactions between the two.