National Library of Energy BETA

Sample records for including persistent stranded

  1. Double stranded nucleic acid biochips

    DOE Patents [OSTI]

    Chernov, Boris; Golova, Julia

    2006-05-23

    This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

  2. Neutrino Physics AAPT Strand Day

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics AAPT Strand Day NSTA Regional, 2005 Jocelyn Monroe, Columbia University 1. What Is a Neutrino Anyway? 2. The Question Of Neutrino Mass 3. Searching For Neutrino Oscillations 4. Where Are We Now? ν Neutrinos, they are very small. They have no charge and have no mass And do not interact at all. The earth is just a silly ball To them, through which they simply pass... ...And pierce the lover and his lass From underneath the bed- you call It wonderful; I call it crass. J from ``Cosmic

  3. PACKAGE INCLUDES:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PACKAGE INCLUDES: Airfare from Seattle, 4 & 5 Star Hotels, Transfers, Select Meals, Guided Tours and Excursions DAY 01: BANGKOK - ARRIVAL DAY 02: BANGKOK - SIGHTSEEING DAY 03: BANGKOK - FLOATING MARKET DAY 04: BANGKOK - AT LEISURE DAY 05: BANGKOK - CHIANG MAI BY AIR DAY 06: CHIANG MAI - SIGHTSEEING DAY 07: CHIANG MAI - ELEPHANT CAMP DAY 08: CHIANG MAI - PHUKET BY AIR DAY 09: PHUKET - PHI PHI ISLAND BY FERRY DAY 10: PHUKET - AT LEISURE DAY 11: PHUKET - CORAL ISLAND BY SPEEDBOAT DAY 12: PHUKET

  4. After Cajun, what next for stranded costs?

    SciTech Connect (OSTI)

    Pembroke, J.D.

    1994-10-01

    Members of FERC have said that the Cajun decision does not pose a serious problem to its proposed rulemaking on stranded costs. But the D.C. Circuit`s Cajun decision, criticizing the concept of recovery for stranded investment in broad terms, promises to make that task difficult, if not impossible. The United States Court of Appeals for the District of Columbia Circuit, in its July 12, 1994 opinion in Cajun Electric Power Cooperative, Inc. v. Federal Energy Regulatory Commission, subjected the Entergy Corporations`s transmission tariff`s stranded investment provision to broad and, perhaps, fatal criticism. The Cajun opinion, premised on basic concepts of antitrust law, was issued at a time of substantial industry discussion on the concept of stranded investment and should greatly impact the decisions of both federal and state regulators on stranded investment.

  5. Solid phase sequencing of double-stranded nucleic acids

    DOE Patents [OSTI]

    Fu, Dong-Jing; Cantor, Charles R.; Koster, Hubert; Smith, Cassandra L.

    2002-01-01

    This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

  6. Method and apparatus for testing a forward-moving strand

    DOE Patents [OSTI]

    Ducommun, Joel; Vulliens, Philippe

    1980-01-01

    In a method for testing a continuously forward-moving strand a light beam which passes along a plane that extends approximately perpendicularly to the longitudinal axis of the strand is introduced into the strand. The brightness value is measured on a place of the strand exterior which is distal from the light incidence place by means of at least one photoelectronic element disposed directly on the strand exterior and the measured result is evaluated in a gating circuit which is electrically connected to the photoelectronic element.

  7. Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel

    1991-01-01

    A method is provided for producing single stranded non-self-complementary nucleic acid probes, and for treating target DNA for use therewith. Probe is constructed by treating DNA with a restriction enzyme and an exonuclease to form template/primers for a DNA polymerase. The digested strand is resynthesized in the presence of labeled nucleoside triphosphate precursor. Labeled single stranded fragments are separated from the resynthesized fragments to form the probe. Target DNA is treated with the same restriction enzyme used to construct the probe, and is treated with an exonuclease before application of the probe. The method significantly increases the efficiency and specificity of hybridization mixtures by increasing effective probe concentration by eliminating self-hybridization between both probe and target DNAs, and by reducing the amount of target DNA available for mismatched hybridizations.

  8. Method of preparing and applying single stranded DNA probes to double stranded target DNAs in situ

    DOE Patents [OSTI]

    Gray, J.W.; Pinkel, D.

    1991-07-02

    A method is provided for producing single stranded non-self-complementary nucleic acid probes, and for treating target DNA for use therewith. The probe is constructed by treating DNA with a restriction enzyme and an exonuclease to form template/primers for a DNA polymerase. The digested strand is resynthesized in the presence of labeled nucleoside triphosphate precursor. Labeled single stranded fragments are separated from the resynthesized fragments to form the probe. Target DNA is treated with the same restriction enzyme used to construct the probe, and is treated with an exonuclease before application of the probe. The method significantly increases the efficiency and specificity of hybridization mixtures by increasing effective probe concentration by eliminating self-hybridization between both probe and target DNAs, and by reducing the amount of target DNA available for mismatched hybridizations. No Drawings

  9. Method for producing labeled single-stranded nucleic acid probes

    DOE Patents [OSTI]

    Dunn, John J.; Quesada, Mark A.; Randesi, Matthew

    1999-10-19

    Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment. More specifically, the method comprises providing a recombinant DNA construct comprising a DNA segment of interest inserted in a cloning vector, the cloning vector having an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment of interest. The recombinant DNA construct is then contacted with the protein pII encoded by gene II of phage f1 thereby generating a single-stranded nick. The nicked DNA is then contacted with E. coli Exonuclease III thereby expanding the single-stranded nick into a single-stranded gap. The single-stranded gapped DNA is then contacted with a single-strand-specific endonuclease thereby producing a linearized DNA molecule containing a double-stranded deletion corresponding in size to the single-stranded gap. The DNA treated in this manner is then incubated with DNA ligase under conditions appropriate for ligation. Also disclosed is a method for producing single-stranded DNA probes. In this embodiment, single-stranded gapped DNA, produced as described above, is contacted with a DNA polymerase in the presence of labeled nucleotides to fill in the gap. This DNA is then linearized by digestion with a restriction enzyme which cuts outside the DNA segment of interest. The product of this digestion is then denatured to produce a labeled single-stranded nucleic acid probe.

  10. Investigation of the persistence of new building commissioning

    SciTech Connect (OSTI)

    Potter, Amanda; Friedman, Hannah; Haasl, Tudi; Claridge, David

    2002-03-17

    Commissioning is gaining increasing recognition as a cost-effective strategy for reducing commercial building energy use. Although the success and cost-effectiveness of commissioning projects depends on how well the benefits of commissioning persist over time, this aspect of commissioning is not well understood. The persistence of commissioning benefits was recently studied in a PIER project evaluating ten buildings that were commissioned at building start-up at least two years ago. The researchers examined the commissioning reports, control algorithms, EMCS point measurements, and energy use data, and conducted operator and commissioning provider interviews to investigate the persistence of commissioning benefits. In addition, they conducted site visits in a sample of the buildings. A set of commissioning measures was selected for each building to compare the persistence of benefits. Persistence was measured both qualitatively through a discussion of occupant comfort and decreased maintenance and quantitatively through estimations of energy savings. This paper reports the results of the study. The discussion includes how well the benefits of commissioning persisted over time, reasons for declining performance, and methods for improving persistence. The results provide valuable insight into how to estimate the persistence of commissioning information central to the cost benefit analyses routinely performed for commissioning measures.

  11. Final Report: MATERIALS, STRANDS, AND CABLES FOR SUPERCONDUCTING ACCELERATOR MAGNETS [Grant Number DE-SC0010312

    SciTech Connect (OSTI)

    Sumption, Mike; Collings, E.

    2014-10-29

    Our program consisted of the two components: Strand Research and Cable Research, with a focus on Nb3Sn, Bi2212, and YBCO for accelerator magnet applications. We demonstrated a method to refine the grains in Nb3Sn by a factor of two, reaching 45 nm grain sizes, and layer Jcs of 6 kA/mm2 at 12 T. W also measured conductor magnetization for field quality. This has been done both with Nb3Sn conductor, as well as Bi:2212 strand. Work in support of quench studies of YBCO coils was also performed. Cable loss studies in Nb3Sn focused on connecting and comparing persistent magnetization and coupling magnetization for considering their relative impact on HEP machines. In the area of HTS cables, we have investigated both the quench in multistrand YBCO CORC cables, as well as the magnetization of these cables for use in high field magnets. In addition, we examined the magnetic and thermal properties of large (50 T) solenoids.

  12. Method for nucleic acid hybridization using single-stranded DNA binding protein

    DOE Patents [OSTI]

    Tabor, Stanley; Richardson, Charles C.

    1996-01-01

    Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

  13. Background meeting and technology demonstration: Chromium electroplating of superconductor strand

    SciTech Connect (OSTI)

    1995-05-01

    A meeting concerned with electroplating copper onto superconducting wires was held. Topics of discussion were concerned with the market, strand cleanliness, and an improved rinsing system for the process.

  14. Unidirectional Scaffold-Strand Arrangement in DNA Origami

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unidirectional Scaffold-Strand Arrangement in DNA Origami Authors: Han, D., Jiang, S., Samanta, A., Liu, Y., and Yan, H. Title: Unidirectional Scaffold-Strand Arrangement in DNA Origami Source: Angewandte Chemie International Edition Year: 2013 Volume: 52 Pages: 9031-9034 ABSTRACT: Date of online publication: Sun, 2013-07-14 Link online: http://onlinelibrary.wiley.com/doi/10.1002/anie.201302177/suppinfo

  15. Pump apparatus including deconsolidator

    DOE Patents [OSTI]

    Sonwane, Chandrashekhar; Saunders, Timothy; Fitzsimmons, Mark Andrew

    2014-10-07

    A pump apparatus includes a particulate pump that defines a passage that extends from an inlet to an outlet. A duct is in flow communication with the outlet. The duct includes a deconsolidator configured to fragment particle agglomerates received from the passage.

  16. Optical modulator including grapene

    DOE Patents [OSTI]

    Liu, Ming; Yin, Xiaobo; Zhang, Xiang

    2016-06-07

    The present invention provides for a one or more layer graphene optical modulator. In a first exemplary embodiment the optical modulator includes an optical waveguide, a nanoscale oxide spacer adjacent to a working region of the waveguide, and a monolayer graphene sheet adjacent to the spacer. In a second exemplary embodiment, the optical modulator includes at least one pair of active media, where the pair includes an oxide spacer, a first monolayer graphene sheet adjacent to a first side of the spacer, and a second monolayer graphene sheet adjacent to a second side of the spacer, and at least one optical waveguide adjacent to the pair.

  17. Materials, Strands, and Cables for Superconducting Accelerator Magnets. Final Report

    SciTech Connect (OSTI)

    Sumption, Mike D.; Collings, Edward W.

    2014-09-19

    This report focuses on Materials, Strands and Cables for High Energy Physics Particle accelerators. In the materials area, work has included studies of basic reactions, diffusion, transformations, and phase assemblage of Nb3Sn. These materials science aspects have been married to results, in the form of flux pinning, Bc2, Birr, and transport Jc, with an emphasis on obtaining the needed Jc for HEP needs. Attention has also been paid to the “intermediate-temperature superconductor”, magnesium diboride emphasis being placed on (i) irreversibility field enhancement, (ii) critical current density and flux pinning, and (iii) connectivity. We also report on studies of Bi-2212. The second area of the program has been in the area of “Strands” in which, aside from the materials aspect of the conductor, its physical properties and their influence on performance have been studied. Much of this work has been in the area of magnetization estimation and flux jump calculation and control. One of the areas of this work was strand instabilities in high-performance Nb3Sn conductors due to combined fields and currents. Additionally, we investigated quench and thermal propagation in YBCO coated conductors at low temperatures and high fields. The last section, “Cables”, focussed on interstrand contact resistance, ICR, it origins, control, and implications. Following on from earlier work in NbTi, the present work in Nb3Sn has aimed to make ICR intermediate between the two extremes of too little contact (no current sharing) and too much (large and unacceptable magnetization and associated beam de-focussing). Interstrand contact and current sharing measurements are being made on YBCO based Roebel cables using transport current methods. Finally, quench was investigated for YBCO cables and the magnets wound from them, presently with a focus on 50 T solenoids for muon collider applications.

  18. An intercalation-locked parallel-stranded DNA tetraplex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tripathi, S.; Zhang, D.; Paukstelis, P. J.

    2015-01-27

    DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson–Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A–A base pairs betweenmore » adjacent G–G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H–1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures.« less

  19. An intercalation-locked parallel-stranded DNA tetraplex

    SciTech Connect (OSTI)

    Tripathi, S.; Zhang, D.; Paukstelis, P. J.

    2015-01-27

    DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson–Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A–A base pairs between adjacent G–G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H–1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures.

  20. Persistent Monitoring Platforms Final Report

    SciTech Connect (OSTI)

    Bennett, C L

    2007-02-22

    This project was inspired and motivated by the need to provide better platforms for persistent surveillance. In the years since the inception of this work, the need for persistence of surveillance platforms has become even more widely appreciated, both within the defense community and the intelligence community. One of the most demanding technical requirements for such a platform involves the power plant and energy storage system, and this project concentrated almost exclusively on the technology associated with this system for a solar powered, high altitude, unmanned aircraft. An important realization for the feasibility of such solar powered aircraft, made at the outset of this project, was that thermal energy may be stored with higher specific energy density than for any other known practical form of rechargeable energy storage. This approach has proved to be extraordinarily fruitful, and a large number of spin-off applications of this technology were developed in the course of this project.

  1. Factors that affect electric-utility stranded commitments

    SciTech Connect (OSTI)

    Hirst, E.; Hadley, S.; Baxter, L.

    1996-07-01

    Estimates of stranded commitments for U.S. investor-owned utilities range widely, with many falling in the range of $100 to $200 billion. These potential losses exist because some utility-owned power plants, long-term power-purchase contracts and fuel-supply contracts, regulatory assets, and expenses for public-policy programs have book values that exceed their expected market values under full competition. This report quantifies the sensitivity of stranded- commitment estimates to the various factors that lead to these above- market-value estimates. The purpose of these sensitivity analyses is to improve understanding on the part of state and federal regulators, utilities, customers, and other electric-industry participants about the relative importance of the factors that affect stranded- commitment amounts.

  2. Method for fabricating multi-strand superconducting cable

    DOE Patents [OSTI]

    Borden, A.R.

    1985-04-01

    Multi-strand superconducting cables adapted to be used, for example, to wind a magnet are fabricated by directing wire strands inwardly from spools disposed on the perimeter of a rotating disk and wrapping them diagonally around a tapered mandrel with a flattened cross-sectional shape with a core having a wedge-shaped channel. As the cable is pulled axially, flexibly coupled wedge-shaped pieces are continuously passed through the channel in the mandrel and inserted into the cable as an internal support therefor.

  3. Phosphors with long-persistent green phosphorescence

    DOE Patents [OSTI]

    Yen, William M; Jia, Weiyi; Lu, Lizhu; Yuan, Huabiao

    2001-01-01

    This invention relates to phosphors including long-persistence green phosphors. Phosphors of the invention are represented by the general formula: M.sub.k Al.sub.2 O.sub.4 :2xEu.sup.2+,2yR.sup.3+ wherein k-1-2x-2y, x is a number ranging from about 0.0001 to about 0.05, y is a number ranging from about x to about 3x, M is an alkaline earth metal, and R.sup.3+ is one or more trivalent metal ions. Phosphors of this invention include powders, ceramics, single crystals and single crystal fibers. A method of manufacturing improved phosphors and a method of manufacturing single crystal phosphors are also provided.

  4. Low latency and persistent data storage

    DOE Patents [OSTI]

    Fitch, Blake G; Franceschini, Michele M; Jagmohan, Ashish; Takken, Todd E

    2014-02-18

    Persistent data storage is provided by a method that includes receiving a low latency store command that includes write data. The write data is written to a first memory device that is implemented by a nonvolatile solid-state memory technology characterized by a first access speed. It is acknowledged that the write data has been successfully written to the first memory device. The write data is written to a second memory device that is implemented by a volatile memory technology. At least a portion of the data in the first memory device is written to a third memory device when a predetermined amount of data has been accumulated in the first memory device. The third memory device is implemented by a nonvolatile solid-state memory technology characterized by a second access speed that is slower than the first access speed.

  5. Low latency and persistent data storage

    DOE Patents [OSTI]

    Fitch, Blake G; Franceschini, Michele M; Jagmohan, Ashish; Takken, Todd

    2014-11-04

    Persistent data storage is provided by a computer program product that includes computer program code configured for receiving a low latency store command that includes write data. The write data is written to a first memory device that is implemented by a nonvolatile solid-state memory technology characterized by a first access speed. It is acknowledged that the write data has been successfully written to the first memory device. The write data is written to a second memory device that is implemented by a volatile memory technology. At least a portion of the data in the first memory device is written to a third memory device when a predetermined amount of data has been accumulated in the first memory device. The third memory device is implemented by a nonvolatile solid-state memory technology characterized by a second access speed that is slower than the first access speed.

  6. Applications of Strand-Specific in situ Hybridization

    SciTech Connect (OSTI)

    Goodwin, E.H.; Meyne, J.; Bailey, S.M.; Quigley, D.; Smith, L.; Tennyson, R.

    1997-01-01

    This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). Fluorescence in situ hybridization (FISH) is used to determine the location of specific DNA sequences on chromosomes. It is an effective tool in genomic mapping and is finding increasing use in medical diagnosis. A ''strand-specific'' version of FISH has been developed in the Life Sciences Division of LANL. The new procedure, named CO-FISH, reveals not only location but also the 5'-to-3'direction of a target sequence, such as the sense strand of a gene. This project was designed to investigate applications of the new technique. Strand-specific FISH was found to be useful and informative for genomic mapping of repetitive DNA sequences. The method provide a valuable new tool for investigating the mechanisms of aneuploidy inducing agents and the cytogenetic phenomena called lateral asymmetry. Finally, using strand-specific FISH, the authors were able to detect certain types of chromosome aberrations (isochromosomes, inversions and Robertsonian translocations) that can be difficult to observe with standard techniques.

  7. Dualities in Persistent (Co)Homology

    SciTech Connect (OSTI)

    de Silva, Vin; Morozov, Dmitriy; Vejdemo-Johansson, Mikael

    2011-09-16

    We consider sequences of absolute and relative homology and cohomology groups that arise naturally for a filtered cell complex. We establishalgebraic relationships between their persistence modules, and show that they contain equivalent information. We explain how one can use the existingalgorithm for persistent homology to process any of the four modules, and relate it to a recently introduced persistent cohomology algorithm. Wepresent experimental evidence for the practical efficiency of the latter algorithm.

  8. Synthetic Aperture Radar Persistent Scatterer Interferometry...

    Open Energy Info (EERE)

    NA, 2010 DOI Not Provided Check for DOI availability: http:crossref.org Online Internet link for Synthetic Aperture Radar Persistent Scatterer Interferometry (PSInSAR)...

  9. Improvements in strand feeding and its effect of sintering performance

    SciTech Connect (OSTI)

    Beer, H.; Kersting, K.; Werner, P.

    1995-12-01

    Sintering may be considered a rather simple, counter current gas-solid process. A bed of granular solids is moved horizontally on a strand of pallets and suction is applied beneath the grate. Shortly after the sinter mix is fed onto the strand the incorporated solid fuel is ignited in the surface layer and the hot gases are drawn into the bed. The temperature of the top layer is raised high enough to burn the fuel particles while air is sucked down through it. Passing the upper, already sintered part of the bed the air is first preheated then sustains the combustion reaction. The hot, still oxygen-rich combustion gases leave the sintering zone and transfer its heat to the charge below. While the solids are preheated, carbonates, combined water, and moisture are driven off, rapidly cooling the gas. Thus, a flame front propagates through the traveling bed, generating at peak temperatures enough heat to agglomerate the bed of quasi-particles into a sinter cake. The strand speed is adjusted so that the burning through of the combustion zone coincides with the end of the suction area. To ensure stable operation this cross stream reactor has to be kept in a steady state.

  10. Probing the Conformational Distributions of Sub-Persistence Length DNA

    SciTech Connect (OSTI)

    Mastroianni, Alexander; Sivak, David; Geissler, Phillip; Alivisatos, Paul

    2009-06-08

    We have measured the bending elasticity of short double-stranded DNA (dsDNA) chains through small-angle X-ray scattering from solutions of dsDNA-linked dimers of gold nanoparticles. This method, which does not require exertion of external forces or binding to a substrate, reports on the equilibrium distribution of bending fluctuations, not just an average value (as in ensemble FRET) or an extreme value (as in cyclization), and in principle provides a more robust data set for assessing the suitability of theoretical models. Our experimental results for dsDNA comprising 42-94 basepairs (bp) are consistent with a simple worm-like chain model of dsDNA elasticity, whose behavior we have determined from Monte Carlo simulations that explicitly represent nanoparticles and their alkane tethers. A persistence length of 50 nm (150 bp) gave a favorable comparison, consistent with the results of single-molecule force-extension experiments on much longer dsDNA chains, but in contrast to recent suggestions of enhanced flexibility at these length scales.

  11. Validation of Finite-Element Models of Persistent-Current Effects in Nb3Sn Accelerator Magnets

    SciTech Connect (OSTI)

    Wang, X.; Ambrosio, G.; Chlachidze, G.; Collings, E. W.; Dietderich, D. R.; DiMarco, J.; Felice, H.; Ghosh, A. K.; Godeke, A.; Gourlay, S. A.; Marchevsky, M.; Prestemon, S. O.; Sabbi, G.; Sumption, M. D.; Velev, G. V.; Xu, X.; Zlobin, A. V.

    2015-01-06

    Persistent magnetization currents are induced in superconducting filaments during the current ramping in magnets. The resulting perturbation to the design magnetic field leads to field quality degradation, in particular at low field where the effect is stronger relative to the main field. The effects observed in NbTi accelerator magnets were reproduced well with the critical-state model. However, this approach becomes less accurate for the calculation of the persistent-current effects observed in Nb3Sn accelerator magnets. Here a finite-element method based on the measured strand magnetization is validated against three state-of-art Nb3Sn accelerator magnets featuring different subelement diameters, critical currents, magnet designs and measurement temperatures. The temperature dependence of the persistent-current effects is reproduced. Based on the validated model, the impact of conductor design on the persistent current effects is discussed. The performance, limitations and possible improvements of the approach are also discussed.

  12. Solid-state NMR analysis of the {beta}-strand orientation of the protofibrils of amyloid {beta}-protein

    SciTech Connect (OSTI)

    Doi, Takashi; Masuda, Yuichi; Irie, Kazuhiro; Akagi, Ken-ichi; Monobe, Youko; Imazawa, Takayoshi; Takegoshi, K.

    2012-11-30

    Highlights: Black-Right-Pointing-Pointer The supramolecular structure of A{beta}42 protofibrils was analyzed by solid-state NMR. Black-Right-Pointing-Pointer The Ala-21 residue in the A{beta}42 protofibrils is included in a slightly disordered {beta}-strand. Black-Right-Pointing-Pointer The A{beta}42 protofibrils do not form intermolecular in-register parallel {beta}-sheets. -- Abstract: Alzheimer's disease (AD) is caused by abnormal deposition (fibrillation) of a 42-residue amyloid {beta}-protein (A{beta}42) in the brain. During the process of fibrillation, the A{beta}42 takes the form of protofibrils with strong neurotoxicity, and is thus believed to play a crucial role in the pathogenesis of AD. To elucidate the supramolecular structure of the A{beta}42 protofibrils, the intermolecular proximity of the Ala-21 residues in the A{beta}42 protofibrils was analyzed by {sup 13}C-{sup 13}C rotational resonance experiments in the solid state. Unlike the A{beta}42 fibrils, an intermolecular {sup 13}C-{sup 13}C correlation was not found in the A{beta}42 protofibrils. This result suggests that the {beta}-strands of the A{beta}42 protofibrils are not in an in-register parallel orientation. A{beta}42 monomers would assemble to form protofibrils with the {beta}-strand conformation, then transform into fibrils by forming intermolecular parallel {beta}-sheets.

  13. Analysis of Strand-Specific RNA-Seq Data Using Machine Learning...

    Office of Scientific and Technical Information (OSTI)

    Analysis of Strand-Specific RNA-Seq Data Using Machine Learning Reveals the Structures of Transcription Units in Clostridium thermocellum Citation Details In-Document Search Title: ...

  14. Multicopy single-stranded DNA directs intestinal colonization of enteric pathogens

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Elfenbein, Johanna R.; Knodler, Leigh A.; Nakayasu, Ernesto S.; Ansong, Charles; Brewer, Heather M.; Bogomolnaya, Lydia; Adams, L. Garry; McClelland, Michael; Adkins, Joshua N.; Andrews-Polymenis, Helene L.; et al

    2015-09-14

    Multicopy single-stranded DNAs (msDNAs) are hybrid RNA-DNA molecules encoded on retroelements called retrons and produced by the action of retron reverse transcriptases. Retrons are widespread in bacteria but the natural function of msDNA has remained elusive despite 30 years of study. The major roadblock to elucidation of the function of these unique molecules has been the lack of any identifiable phenotypes for mutants unable to make msDNA. We report that msDNA of the zoonotic pathogen Salmonella Typhimurium is necessary for colonization of the intestine. Similarly, we observed a defect in intestinal persistence in an enteropathogenic E. coli mutant lacking itsmore » retron reverse transcriptase. Under anaerobic conditions in the absence of msDNA, proteins of central anaerobic metabolism needed for Salmonella colonization of the intestine are dysregulated. We show that the msDNA-deficient mutant can utilize nitrate, but not other alternate electron acceptors in anaerobic conditions. Consistent with the availability of nitrate in the inflamed gut, a neutrophilic inflammatory response partially rescued the ability of a mutant lacking msDNA to colonize the intestine. These findings together indicate that the mechanistic basis of msDNA function during Salmonella colonization of the intestine is proper production of proteins needed for anaerobic metabolism. We further conclude that a natural function of msDNA is to regulate protein abundance, the first attributable function for any msDNA. Our data provide novel insight into the function of this mysterious molecule that likely represents a new class of regulatory molecules.« less

  15. Multicopy single-stranded DNA directs intestinal colonization of enteric pathogens

    SciTech Connect (OSTI)

    Elfenbein, Johanna R.; Knodler, Leigh A.; Nakayasu, Ernesto S.; Ansong, Charles; Brewer, Heather M.; Bogomolnaya, Lydia; Adams, L. Garry; McClelland, Michael; Adkins, Joshua N.; Andrews-Polymenis, Helene L.; Fang, Ferric C.

    2015-09-14

    Multicopy single-stranded DNAs (msDNAs) are hybrid RNA-DNA molecules encoded on retroelements called retrons and produced by the action of retron reverse transcriptases. Retrons are widespread in bacteria but the natural function of msDNA has remained elusive despite 30 years of study. The major roadblock to elucidation of the function of these unique molecules has been the lack of any identifiable phenotypes for mutants unable to make msDNA. We report that msDNA of the zoonotic pathogen Salmonella Typhimurium is necessary for colonization of the intestine. Similarly, we observed a defect in intestinal persistence in an enteropathogenic E. coli mutant lacking its retron reverse transcriptase. Under anaerobic conditions in the absence of msDNA, proteins of central anaerobic metabolism needed for Salmonella colonization of the intestine are dysregulated. We show that the msDNA-deficient mutant can utilize nitrate, but not other alternate electron acceptors in anaerobic conditions. Consistent with the availability of nitrate in the inflamed gut, a neutrophilic inflammatory response partially rescued the ability of a mutant lacking msDNA to colonize the intestine. These findings together indicate that the mechanistic basis of msDNA function during Salmonella colonization of the intestine is proper production of proteins needed for anaerobic metabolism. We further conclude that a natural function of msDNA is to regulate protein abundance, the first attributable function for any msDNA. Our data provide novel insight into the function of this mysterious molecule that likely represents a new class of regulatory molecules.

  16. Overcoming Persistent Barriers to Energy Efficiency in Multifamily...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing through Partnerships Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing through ...

  17. Emergence of the Persistent Spin Helix in Semiconductor Quantum...

    Office of Scientific and Technical Information (OSTI)

    Emergence of the Persistent Spin Helix in Semiconductor Quantum Wells Citation Details In-Document Search Title: Emergence of the Persistent Spin Helix in Semiconductor Quantum ...

  18. Validation of Finite-Element Models of Persistent-Current Effects in Nb3Sn Accelerator Magnets

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wang, X.; Ambrosio, G.; Chlachidze, G.; Collings, E. W.; Dietderich, D. R.; DiMarco, J.; Felice, H.; Ghosh, A. K.; Godeke, A.; Gourlay, S. A.; et al

    2015-01-06

    Persistent magnetization currents are induced in superconducting filaments during the current ramping in magnets. The resulting perturbation to the design magnetic field leads to field quality degradation, in particular at low field where the effect is stronger relative to the main field. The effects observed in NbTi accelerator magnets were reproduced well with the critical-state model. However, this approach becomes less accurate for the calculation of the persistent-current effects observed in Nb3Sn accelerator magnets. Here a finite-element method based on the measured strand magnetization is validated against three state-of-art Nb3Sn accelerator magnets featuring different subelement diameters, critical currents, magnet designsmore » and measurement temperatures. The temperature dependence of the persistent-current effects is reproduced. Based on the validated model, the impact of conductor design on the persistent current effects is discussed. The performance, limitations and possible improvements of the approach are also discussed.« less

  19. MHK technology developments include current

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    technology developments include current energy conversion (CEC) devices, for example, hydrokinetic turbines that extract power from water currents (riverine, tidal, and ocean) and wave energy conversion (WEC) devices that extract power from wave motion. Sandia's MHK research leverages decades of experience in engineering, design, and analysis of wind power technologies, and its vast research complex, including high- performance computing (HPC), advanced materials and coatings, nondestructive

  20. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Majoros, M.; Sumption, M. D.; Collings, E. W.; Long, N. J.

    2015-04-08

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of I–V curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software.more » Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 mΩ, (enabling a current sharing of 5% at 1.3Ic) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 100–1000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.« less

  1. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    sumption, Mike; Majoros, Milan; Collings, E. W.; Van der Laan, D. C.

    2014-11-07

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of I–V curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software.more » Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 mΩ, (enabling a current sharing of 5% at 1.3Ic ) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 100–1000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.« less

  2. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    SciTech Connect (OSTI)

    Majoros, M.; Sumption, M. D.; Collings, E. W.; Long, N. J.

    2015-04-08

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of I–V curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software. Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 mΩ, (enabling a current sharing of 5% at 1.3Ic) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 100–1000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.

  3. Inter-strand current sharing and ac loss measurements in superconducting YBCO Roebel cables

    SciTech Connect (OSTI)

    sumption, Mike; Majoros, Milan; Collings, E. W.; Van der Laan, D. C.

    2014-11-07

    A Roebel cable, one twist pitch long, was modified from its as-received state by soldering copper strips between the strands to provide inter-strand connections enabling current sharing. Various DC transport currents (representing different percentages of its critical current) were applied to a single strand of such a modified cable at 77 K in a liquid nitrogen bath. Simultaneous monitoring of IV curves in different parts of the strand as well as in its interconnections with other strands was made using a number of sensitive Keithley nanovoltmeters in combination with a multichannel high-speed data acquisition card, all controlled via LabView software. Current sharing onset was observed at about 1.02 of strand Ic. At a strand current of 1.3Ic about 5% of the current was shared through the copper strip interconnections. A finite element method modeling was performed to estimate the inter-strand resistivities required to enable different levels of current sharing. The relative contributions of coupling and hysteretic magnetization (and loss) were compared, and for our cable and tape geometry, and at dB/dt=1 T s-1, and our inter-strand resistance of 0.77 m?, (enabling a current sharing of 5% at 1.3Ic ) the coupling component was 0.32% of the hysteretic component. However, inter-strand contact resistance values of 1001000 times smaller (close to those of NbTi and Nb3Sn based accelerator cables) would make the coupling components comparable in size to the hysteretic components.

  4. Characteristics of Cu stabilized Nb3Al strands with low Cu ratio

    SciTech Connect (OSTI)

    Kikuchi, A.; Yamada, R.; Barzi, E.; Kobayashi, M.; Lamm, M.; Nakagawa, K.; Sasaki, K.; Takeuchi, T.; Turrioni, D.; Zlobin, A.V.; /NIMC, Tsukuba /Fermilab /Hitachi, Tsuchiura Works /KEK, Tsukuba

    2008-12-01

    Characteristics of recently developed F4-Nb{sub 3}Al strand with low Cu ratio are described. The overall J{sub c} of the Nb{sub 3}Al strand could be easily increased by decreasing of the Cu ratio. Although the quench of a pulse-like voltage generation is usually observed in superconducting unstable conductor, the F4 strand with a low Cu ratio of 0.61 exhibited an ordinary critical transition of gradual voltage generation. The F4 strand does not have magnetic instabilities at 4.2 K because of the tantalum interfilament matrix. The overall J{sub c} of the F4 strand achieved was 80-85% of the RRP strand. In the large mechanical stress above 100 MPa, the overall J{sub c} of the F4 strand might be comparable to that of high J{sub c} RRP-Nb{sub 3}Sn strands. The Rutherford cable with a high packing factor of 86.5% has been fabricated using F4 strands. The small racetrack magnet, SR07, was also fabricated by a 14 m F4 cable. The quench current, I{sub q}, of SR07 were obtained 22.4 kA at 4.5 K and 25.2 kA at 2.2 K. The tantalum matrix Nb{sub 3}Al strands are promising for the application of super-cooled high-field magnets as well as 4.2 K operation magnets.

  5. Persistence of soil organic matter as an ecosystem property

    SciTech Connect (OSTI)

    Schmidt, M.W.; Torn, M. S.; Abiven, S.; Dittmar, T.; Guggenberger, G.; Janssens, I.A.; Kleber, M.; Kgel-Knabner, I.; Lehmann, J.; Manning, D.A.C.; Nannipieri, P.; Rasse, D.P.; Weiner, S.; Trumbore, S.E.

    2011-08-15

    Globally, soil organic matter (SOM) contains more than three times as much carbon as either the atmosphere or terrestrial vegetation. Yet it remains largely unknown why some SOM persists for millennia whereas other SOM decomposes readilyand this limits our ability to predict how soils will respond to climate change. Recent analytical and experimental advances have demonstrated that molecular structure alone does not control SOM stability: in fact, environmental and biological controls predominate. Here we propose ways to include this understanding in a new generation of experiments and soil carbon models, thereby improving predictions of the SOM response to global warming.

  6. UAV Cooperation Architectures for Persistent Sensing

    SciTech Connect (OSTI)

    Roberts, R S; Kent, C A; Jones, E D

    2003-03-20

    With the number of small, inexpensive Unmanned Air Vehicles (UAVs) increasing, it is feasible to build multi-UAV sensing networks. In particular, by using UAVs in conjunction with unattended ground sensors, a degree of persistent sensing can be achieved. With proper UAV cooperation algorithms, sensing is maintained even though exceptional events, e.g., the loss of a UAV, have occurred. In this paper a cooperation technique that allows multiple UAVs to perform coordinated, persistent sensing with unattended ground sensors over a wide area is described. The technique automatically adapts the UAV paths so that on the average, the amount of time that any sensor has to wait for a UAV revisit is minimized. We also describe the Simulation, Tactical Operations and Mission Planning (STOMP) software architecture. This architecture is designed to help simulate and operate distributed sensor networks where multiple UAVs are used to collect data.

  7. COHERENT STRUCTURES IN PLASMA TURBULENCE: PERSISTENCE, INTERMITTENCY,

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    COHERENT STRUCTURES IN PLASMA TURBULENCE: PERSISTENCE, INTERMITTENCY, AND CONNECTIONS WITH OBSERVATIONS by Kurt W. Smith A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Physics) at the UNIVERSITY OF WISCONSIN-MADISON 2011 c Copyright by Kurt W. Smith 2011 All Rights Reserved i To Paul Terry, my adviser: for your patient guidance and helpful instruction; for the stimulating conversations and for honing my physical intuition; and for

  8. Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress

    SciTech Connect (OSTI)

    Su, Fengtao; Mukherjee, Shibani; Yang, Yanyong; Mori, Eiichiro; Bhattacharya, Souparno; Kobayashi, Junya; Yannone, Steven  M.; Chen, David  J.; Asaithamby, Aroumougame

    2014-11-20

    WRN, the protein defective in Werner syndrome (WS), is a multifunctional nuclease involved in DNA damage repair, replication, and genome stability maintenance. It was assumed that the nuclease activities of WRN were critical for these functions. Here, we report a nonenzymatic role for WRN in preserving nascent DNA strands following replication stress. We found that lack of WRN led to shortening of nascent DNA strands after replication stress. Furthermore, we discovered that the exonuclease activity of MRE11 was responsible for the shortening of newly replicated DNA in the absence of WRN. Mechanistically, the N-terminal FHA domain of NBS1 recruits WRN to replication-associated DNA double-stranded breaks to stabilize Rad51 and to limit the nuclease activity of its C-terminal binding partner MRE11. Thus, this previously unrecognized nonenzymatic function of WRN in the stabilization of nascent DNA strands sheds light on the molecular reason for the origin of genome instability in WS individuals.

  9. Magnetization anomaly of Nb3Al strands and instability of Nb3Al Rutherford cables

    SciTech Connect (OSTI)

    Yamada, Ryuji; Kikuchi, Akihiro; Wake, Masayoshi; /KEK, Tsukuba

    2006-08-01

    Using a Cu stabilized Nb{sub 3}Al strand with Nb matrix, a 30 meter long Nb{sub 3}Al Rutherford cable was made by a collaboration of Fermilab and NIMS. Recently the strand and cable were tested. In both cases instability was observed at around 1.5 Tesla. The magnetization of this Nb{sub 3}Al strand was measured first using a balanced coil magnetometer at 4.2 K. Strands showed an anomalously large magnetization behavior around at 1.6 T, which is much higher than the usual B{sub c2} {approx} 0.5 Tesla (4.2 K) of Nb matrix. This result is compared with the magnetization data of short strand samples using a SQUID magnetometer, in which a flux-jump signal was observed at 0.5 Tesla, but not at higher field. As a possible explanation for this magnetization anomaly, the interfilament coupling through the thin Nb films in the strands is suggested. The instability problem observed in low field tests of the Nb{sub 3}Al Rutherford cables is attributed to this effect.

  10. Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence:

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Literature Review and DOE-LM Site Surveys | Department of Energy Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence: Literature Review and DOE-LM Site Surveys Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence: Literature Review and DOE-LM Site Surveys Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence: Literature Review and DOE-LM Site Surveys Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence: Literature Review

  11. DNA Persistence in Sink Drain Environment

    SciTech Connect (OSTI)

    Winder, Eric M.; Bonheyo, George T.

    2015-07-31

    Biofilms are organized structures composed mainly of cells and extracellular polymeric substances produced by the constituent microorganisms. Ubiquitous in nature, biofilms have an innate ability to capture and retain passing material and may therefore act as natural collectors of contaminants or signatures of upstream activities. To determine the persistence and detectability of DNA passing through a sink drain environment, Bacillus anthracis strain Ames35 was cultured (6.35 x 107 CFU/mL), sterilized, and disposed of by addition to a sink drain apparatus with an established biofilm.

  12. Investigation of structure and properties of the Nb rods manufactured by different deformation and heat treatment regimes in mass production conditions for the Nb{sub 3}Sn strands

    SciTech Connect (OSTI)

    Abdyukhanov, I. M.; Vorobieva, A. E.; Alekseev, M. V.; Mareev, K. A.; Dergunova, E. A.; Peredkova, T. N. [JSC Bochvar High-Technology Research Institute of Inorganic Materials, 5a Rogova St., Moscow, 123060 (Russian Federation); Shikov, A. K. [NRC Kurchatov Institute, 1 Akademika Kurchatova Sq., Moscow, 123182 (Russian Federation); Utkin, K. V.; Vorobieva, A. V.; Kharkovsky, D. N. [JSC Chepetsky Mechanical Plant, 7 Belova St., Glazov, 427620 (Russian Federation)

    2014-01-27

    From 2009 the mass production of the Nb{sub 3}Sn strands for ITER with the yield of several tens of tons per year operates at JSC Chepetsky Mechanical Plant (Glazov, Russia). In order to enhance the stability of output characteristics of the produced Nb{sub 3}Sn strands, to increase the Nb filaments dimensional homogeneity the manufacture regimes improvement of the used semiproducts such as Nb rods intended for the superconducting filaments formation in the finished strands has been carried out. In the work the investigations of the Nb rheological behavior, the influence of heat treatment in the wide temperature range from 700 to 1300 C on the predeformed Nb rods structure and mechanical properties have been performed. Different production routes of the Nb rods, including such operations like forging, extrusion and drawing combined with the recrystallization annealings, were used. Composite Nb{sub 3}Sn strands have been produced and their electrophysical properties have been tested. For the first time influence of the niobium rods manufacture regimes on the current carrying capacity of the industrial Nb{sub 3}Sn strands has been investigated.

  13. DNA Persistence in Sink Drain Environment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Winder, Eric M.; Bonheyo, George T.

    2015-07-31

    Biofilms are organized structures composed mainly of cells and extracellular polymeric substances produced by the constituent microorganisms. Ubiquitous in nature, biofilms have an innate ability to capture and retain passing material and may therefore act as natural collectors of contaminants or signatures of upstream activities. To determine the persistence and detectability of DNA passing through a sink drain environment, Bacillus anthracis strain Ames35 was cultured (6.35 x 107 CFU/mL), sterilized, and disposed of by addition to a sink drain apparatus with an established biofilm. The sink drain apparatus was sampled before and for several days after the addition of themore » sterilized B. anthracis culture to detect the presence of B. anthracis DNA. Multiple PCR primer pairs were used to screen for chromosomal and plasmid DNA with primers targeting shorter sequences showing greater amplification efficiency and success. PCR amplification and detection of target sequences indicate persistence of chromosomal DNA and plasmid DNA in the biofilm for 5 or more and 14 or more days, respectively.« less

  14. Manganese oxide helices, rings, strands, and films, and methods for their preparation

    DOE Patents [OSTI]

    Suib, Steven L.; Giraldo, Oscar; Marquez, Manuel; Brock, Stephanie

    2003-01-07

    Methods for the preparation of mixed-valence manganese oxide compositions with quaternary ammonium ions are described. The compositions self-assemble into helices, rings, and strands without any imposed concentration gradient. These helices, rings, and strands, as well as films having the same composition, undergo rapid ion exchange to replace the quaternary ammonium ions with various metal ions. And the metal-ion-containing manganese oxide compositions so formed can be heat treated to form semi-conducting materials with high surface areas.

  15. Cytomegalovirus pp65 limits dissemination but is dispensable for persistence

    SciTech Connect (OSTI)

    Malouli, Daniel; Hansen, Scott G.; Nakayasu, Ernesto S.; Marshall, Emily E.; Hughes, Colette M.; Ventura, Abigail B.; Gilbride, Roxanne M.; Lewis, Matthew S.; Xu, Guangwu; Kreklywich, Craig N.; Whizin, Nathan; Fischer, Miranda; Legasse, Alfred W.; Viswanathan, Kasinath; Siess, Don; Camp, David G.; Axthelm, Michael K.; Kahl, Christoph; DeFilippis, Victor R.; Smith, Richard D.; Streblow, Daniel N.; Picker, Louis J.; Fruh, Klaus

    2014-05-01

    The tegument phosphoprotein pp65 (UL83) is the most abundant virion protein in human cytomegalovirus (HCMV). Since pp65 is immunodominant in persistently infected individuals, subunit vaccines against HCMV often include pp65 as T cell stimulatory component. Although HCMV pp65 is non-essential for viral growth in vitro it is thought to have an important role in primary and persistent infection since pp65 displays multiple immunomodulatory functions. To determine whether pp65 is required for infection and to evaluate its role in natural and vaccination-induced immunity we generated a rhesus CMV lacking both homologues, pp65a (Rh111) and pp65b (Rh112). Lack of pp65 resulted in a slight growth defect in vitro and an increase of defective particle formation. However, most pp65-deleted virions in the supernatant were phenotypically normal and proteomics analysis revealed that the ratios of the remaining viral proteins were largely unchanged. RhCMV ?pp65ab was able to persistently infect CMV-negative rhesus macaques (RM) and to super-infect RM previously infected with CMV. To determine whether T cells against pp65 are essential for protection against CMV, we challenged ?pp65ab-infected animals with RhCMV ?US2-11, a viral recombinant that lacks inhibitors of MHC-I antigen presentation and is thus unable to overcome CMV-specific T cell immunity. Despite a complete lack of pp65-specific T cells, ?pp65ab protected against ?US2-11 challenge suggesting that pp65-specific T cells are not essential for T cell immunity against CMV. Using the same approach we further demonstrate that pp65b-specific T cells, induced by heterologous prime/boost vaccination, are not sufficient to protect against ?US2-11 challenge. Our data provides a new approach to test the efficacy of subunit vaccine candidates and suggest that pp65 vaccines are insufficient to induce a T cell response that recapitulates the protective effect of natural infection.

  16. Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence...

    Energy Savers [EERE]

    Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence: Literature Review and DOE-LM Site Surveys Uranium-Bearing Evaporite Mineralization Influencing Plume ...

  17. Persistence of Hydrologic Variables and Reactive Stream Solute...

    Office of Scientific and Technical Information (OSTI)

    Watershed Citation Details In-Document Search Title: Persistence of Hydrologic Variables and Reactive Stream Solute Concentrations in an East Tennessee Watershed Time and ...

  18. "Thinking" Telescopes: An Autonomous Robotic Ecosystem for Persistent...

    Office of Scientific and Technical Information (OSTI)

    Conference: "Thinking" Telescopes: An Autonomous Robotic Ecosystem for Persistent Monitoring and Real-Time Response Citation Details In-Document Search Title: "Thinking"...

  19. "Thinking" Telescopes: An Autonomous Robotic Ecosystem for Persistent...

    Office of Scientific and Technical Information (OSTI)

    Telescopes: An Autonomous Robotic Ecosystem for Persistent Monitoring and Real-Time Response Citation Details In-Document Search Title: "Thinking" Telescopes: An...

  20. Persistent source influences on the trailing edge of a groundwater...

    Office of Scientific and Technical Information (OSTI)

    Persistent source influences on the trailing edge of a groundwater plume, and natural attenuation timeframes: The F-Area Savannah River Site Citation Details In-Document Search ...

  1. Nb3Sn RRP® strand and Rutherford cable development for a 15 T dipole demonstrator

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Barzi, E.; Andreev, N.; Li, P.; Lombardo, V.; Turrioni, D.; Zlobin, A. V.

    2016-03-16

    Keystoned Rutherford cables made of 28 strands and with a stainless steel core were developed and manufactured using 1 mm Nb3Sn composite wires produced by Oxford Superconducting Technology with 127 and 169 restacks using the Restacked-Rod-Process®. Furthermore, the performance and properties of these cables were studied to evaluate possible candidates for 15 T accelerator magnets.

  2. Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Su, Fengtao; Mukherjee, Shibani; Yang, Yanyong; Mori, Eiichiro; Bhattacharya, Souparno; Kobayashi, Junya; Yannone, Steven  M.; Chen, David  J.; Asaithamby, Aroumougame

    2014-11-20

    WRN, the protein defective in Werner syndrome (WS), is a multifunctional nuclease involved in DNA damage repair, replication, and genome stability maintenance. It was assumed that the nuclease activities of WRN were critical for these functions. Here, we report a nonenzymatic role for WRN in preserving nascent DNA strands following replication stress. We found that lack of WRN led to shortening of nascent DNA strands after replication stress. Furthermore, we discovered that the exonuclease activity of MRE11 was responsible for the shortening of newly replicated DNA in the absence of WRN. Mechanistically, the N-terminal FHA domain of NBS1 recruits WRNmore » to replication-associated DNA double-stranded breaks to stabilize Rad51 and to limit the nuclease activity of its C-terminal binding partner MRE11. Thus, this previously unrecognized nonenzymatic function of WRN in the stabilization of nascent DNA strands sheds light on the molecular reason for the origin of genome instability in WS individuals.« less

  3. Zinc chromate induces chromosome instability and DNA double strand breaks in human lung cells

    SciTech Connect (OSTI)

    Xie Hong; Holmes, Amie L.; Young, Jamie L.; Qin Qin; Joyce, Kellie; Pelsue, Stephen C.; Peng Cheng; Wise, Sandra S.; Jeevarajan, Antony S.; Wallace, William T.; Hammond, Dianne; Wise, John Pierce E-mail: John.Wise@usm.maine.edu

    2009-02-01

    Hexavalent chromium Cr(VI) is a respiratory toxicant and carcinogen, with solubility playing an important role in its carcinogenic potential. Zinc chromate, a water insoluble or 'particulate' Cr(VI) compound, has been shown to be carcinogenic in epidemiology studies and to induce tumors in experimental animals, but its genotoxicity is poorly understood. Our study shows that zinc chromate induced concentration-dependent increases in cytotoxicity, chromosome damage and DNA double strand breaks in human lung cells. In response to zinc chromate-induced breaks, MRE11 expression was increased and ATM and ATR were phosphorylated, indicating that the DNA double strand break repair system was initiated in the cells. In addition, our data show that zinc chromate-induced double strand breaks were only observed in the G2/M phase population, with no significant amount of double strand breaks observed in G1 and S phase cells. These data will aid in understanding the mechanisms of zinc chromate toxicity and carcinogenesis.

  4. Making web annotations persistent over time

    SciTech Connect (OSTI)

    Sanderson, Robert; Van De Sompel, Herbert

    2010-01-01

    As Digital Libraries (DL) become more aligned with the web architecture, their functional components need to be fundamentally rethought in terms of URIs and HTTP. Annotation, a core scholarly activity enabled by many DL solutions, exhibits a clearly unacceptable characteristic when existing models are applied to the web: due to the representations of web resources changing over time, an annotation made about a web resource today may no longer be relevant to the representation that is served from that same resource tomorrow. We assume the existence of archived versions of resources, and combine the temporal features of the emerging Open Annotation data model with the capability offered by the Memento framework that allows seamless navigation from the URI of a resource to archived versions of that resource, and arrive at a solution that provides guarantees regarding the persistence of web annotations over time. More specifically, we provide theoretical solutions and proof-of-concept experimental evaluations for two problems: reconstructing an existing annotation so that the correct archived version is displayed for all resources involved in the annotation, and retrieving all annotations that involve a given archived version of a web resource.

  5. Metallographic autopsies of full-scale ITER prototype cable-in-conduit conductors after full cyclic testing in SULTAN: II. Significant reduction of strand movement and strand damage in short twist pitch CICCs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sanabria, Charlos; Lee, Peter J.; Starch, William; Devred, Arnaud; Larbalestier, David C.

    2015-10-14

    Prototype cable in conduit conductors (CICCs) destined for use in the Toroidal Field (TF) and Central Solenoid (CS) coils of the ITER experimental fusion reactor underwent severe cyclic loading in the SULTAN facility. Their autopsies revealed significant and permanent transverse strand migration due to the large Lorentz forces of the SULTAN test. The movement resulted in a 3 7% void fraction increase on the Low Pressure (LP) side of the longer twist pitch CICCs. However, short twist pitch conductors exhibited less than 1% void fraction increase in the LP side, as well as a complete absence of the Nb3Sn filamentmore » fractures observed in the longer twist pitch conductors. We report here a detailed strand to cable analysis of short and longer “baseline” twist pitch CICCs. It was found that the use of Internal Tin strands in the longer “baseline” twist pitch CICCs can be beneficial possibly because of their superior stiffness—which better resist strand movement—while the use of Bronze Process strands showed more movement and poorer cyclic test performance. This was not the case for the short twist pitch CICC. Such conductor design seems to work well with both strand types. But it was found that despite the absence of filament fractures, the short twist pitch CICC made from the Internal Tin strands studied here developed severe strand distortion during cabling which resulted in diffusion barrier breaks and Sn contamination of the Cu stabilizer during the heat treatment. Furthermore, the short twist pitch CICC made from Bronze Process strands preserved diffusion barrier integrity.« less

  6. Quantitative Analysis of Biofuel Sustainability, Including Land...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Quantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG Emissions Quantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG Emissions ...

  7. Topic A Note: Includes STEPS Subtopic

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Topic A Note: Includes STEPS Subtopic 33 Total Projects Developing and Enhancing Workforce Training Programs

  8. Spin transport and spin polarization properties in double-stranded DNA

    SciTech Connect (OSTI)

    Simchi, Hamidreza; Esmaeilzadeh, Mahdi Mazidabadi, Hossein

    2013-11-21

    We study the spin-dependent electron transport through a double-stranded DNA (dsDNA) using the Bogoliubov-de Gennes equations and non-equilibrium Green's function method. We calculate the spin-dependent electron conductance and spin-polarization for different lengths, helix angles, twist angles of dsDNA, the environment-induced dephasing factors, and hopping integral. It is shown that the conductance decreases by increasing the length and dephasing factor. Also, we show that the spin-polarization depends on the helical symmetry and the length of DNA. It is shown that the double-stranded DNA can act as a perfect spin filter. Finally, we show that the sign of spin polarization can be inverted from +1 (−1) to −1 (+1) for some values of hopping integral.

  9. Evaluation of Juvenile Fall Chinook Stranding on the Hanford Reach, 1997-1999 Interim Report.

    SciTech Connect (OSTI)

    Wagner, Paul; Nugent, John; Price, William

    1999-02-15

    Pilot work conducted in 1997 to aid the development of the study for the 1998 Evaluation of Juvenile Fall Chinook Stranding on The Hanford Reach. The objectives of the 1997 work were to: (1) identify juvenile chinook production and rearing areas..., (2) identify sampling sites and develop the statistical parameters necessary to complete the study, (3) develop a study plan..., (4) conduct field sampling activities...

  10. Screening Tool for Providers of Double-Stranded DNA - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Startup America Startup America Industrial Technologies Industrial Technologies Energy Analysis Energy Analysis Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Screening Tool for Providers of Double-Stranded DNA Oak Ridge National Laboratory Contact ORNL About This Technology Publications: PDF Document Publication 11-G00227_ID2510 (2).pdf (829 KB) Technology Marketing SummaryThe invention screens purchase orders submitted to DNA synthesis

  11. Methods to estimate stranded commitments for a restructuring US electricity industry

    SciTech Connect (OSTI)

    Hirst, E.; Hadley, S.; Baxter, L.

    1996-01-01

    Estimates of stranded commitments for US investor-owned electric utilities range widely, from as little as $20 billion to as much as $500 billion (more than double the shareholder equity in US utilities). These potential losses are a consequence of the above-market book values for some utility-owned power plants, long-term power-purchase contracts, deferred income taxes, regulatory assets, and public-policy programs. Because of the wide range of estimates and the potentially large dollar amounts involved, state and federal regulators need a clear understanding of the methods used to calculate these estimates. In addition, they may want simple methods that they can use to check the reasonableness of the estimates that utilities and other parties present in regulatory proceedings. This report explains various top-down and bottom-up methods to calculate stranded commitments. The purpose of this analysis is to help regulators and others understand the implications of different analytical approaches to estimating stranded-commitment amounts. Top-down methods, because they use the utility as the unit of analysis, are simple to apply and to understand. However, their aggregate nature makes it difficult to determine what specific assets and liabilities affect their estimates. Bottom-up methods use the individual asset (e.g., power plant) or liability (e.g., power-purchase contract, fuel-supply contract, and deferred income taxes) as the unit of analysis. These methods have substantial data and computational requirements.

  12. Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence.

    Office of Scientific and Technical Information (OSTI)

    Literature Review and DOE-LM Site Surveys (Technical Report) | SciTech Connect Technical Report: Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence. Literature Review and DOE-LM Site Surveys Citation Details In-Document Search Title: Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence. Literature Review and DOE-LM Site Surveys This report on evaporite mineralization was completed as an Ancillary Work Plan for the Applied Studies and Technology program

  13. Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    through Partnerships | Department of Energy Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing through Partnerships Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing through Partnerships This presentation, held on Jan. 30, 2014, provides information on how to leverage state policies and programs to advance multifamily energy efficiency efforts. Presentation (12.16 MB) Transcript (135 KB) More Documents & Publications HIA ZERH Judge Bios

  14. Evaluating Chemical Persistence in a Multimedia Environment: ACART Analysis

    SciTech Connect (OSTI)

    Bennett, D.H.; McKone, T.E.; Kastenberg, W.E.

    1999-02-01

    For the thousands of chemicals continuously released into the environment, it is desirable to make prospective assessments of those likely to be persistent. Persistent chemicals are difficult to remove if adverse health or ecological effects are later discovered. A tiered approach using a classification scheme and a multimedia model for determining persistence is presented. Using specific criteria for persistence, a classification tree is developed to classify a chemical as ''persistent'' or ''non-persistent'' based on the chemical properties. In this approach, the classification is derived from the results of a standardized unit world multimedia model. Thus, the classifications are more robust for multimedia pollutants than classifications using a single medium half-life. The method can be readily implemented and provides insight without requiring extensive and often unavailable data. This method can be used to classify chemicals when only a few properties are known and be used to direct further data collection. Case studies are presented to demonstrate the advantages of the approach.

  15. The effect of a magnetic field on the spin-selective transport in double-stranded DNA

    SciTech Connect (OSTI)

    Simchi, Hamidreza; Esmaeilzadeh, Mahdi Mazidabadi, Hossein

    2014-05-28

    Spin-polarization in double-stranded DNA is studied in the presence of a magnetic field applied along its helix axis using the non-equilibrium Green's function method. The spin-polarization could be tuned by changing the magnetic field. In some special cases, the double-stranded DNA behaved as a perfect spin-filter. Furthermore, the dependency of the spin-polarization on the spin-orbit strength and dephasing strength is studied.

  16. INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; RADIATION

    Office of Scientific and Technical Information (OSTI)

    interval technical basis document Chiaro, P.J. Jr. 44 INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; RADIATION DETECTORS; RADIATION MONITORS; DOSEMETERS;...

  17. Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs

    SciTech Connect (OSTI)

    Prins, Kathleen C.; Delpeut, Sebastien; Leung, Daisy W.; Reynard, Olivier; Volchkova, Valentina A.; Reid, St. Patrick; Ramanan, Parameshwaran; Cárdenas, Washington B.; Amarasinghe, Gaya K.; Volchkov, Viktor E.; Basler, Christopher F.

    2010-10-11

    Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-{alpha}/{beta} responses that also functions as a viral polymerase cofactor. Recent structural studies identified key features, including a central basic patch, required for VP35 dsRNA binding activity. To address the functional significance of these VP35 structural features for EBOV replication and pathogenesis, two point mutations, K319A/R322A, that abrogate VP35 dsRNA binding activity and severely impair its suppression of IFN-{alpha}/{beta} production were identified. Solution nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography reveal minimal structural perturbations in the K319A/R322A VP35 double mutant and suggest that loss of basic charge leads to altered function. Recombinant EBOVs encoding the mutant VP35 exhibit, relative to wild-type VP35 viruses, minimal growth attenuation in IFN-defective Vero cells but severe impairment in IFN-competent cells. In guinea pigs, the VP35 mutant virus revealed a complete loss of virulence. Strikingly, the VP35 mutant virus effectively immunized animals against subsequent wild-type EBOV challenge. These in vivo studies, using recombinant EBOV viruses, combined with the accompanying biochemical and structural analyses directly correlate VP35 dsRNA binding and IFN inhibition functions with viral pathogenesis. Moreover, these studies provide a framework for the development of antivirals targeting this critical EBOV virulence factor.

  18. Evaluating multimedia chemical persistence: Classification and regression tree analysis

    SciTech Connect (OSTI)

    Bennett, D.H.; McKone, T.E.; Kastenberg, W.E.

    2000-04-01

    For the thousands of chemicals continuously released into the environment, it is desirable to make prospective assessments of those likely to be persistent. Widely distributed persistent chemicals are impossible to remove from the environment and remediation by natural processes may take decades, which is problematic if adverse health or ecological effects are discovered after prolonged release into the environment. A tiered approach using a classification scheme and a multimedia model for determining persistence is presented. Using specific criteria for persistence, a classification tree is developed to classify a chemical as persistent or nonpersistent based on the chemical properties. In this approach, the classification is derived from the results of a standardized unit world multimedia model. Thus, the classifications are more robust for multimedia pollutants than classifications using a single medium half-life. The method can be readily implemented and provides insight without requiring extensive and often unavailable data. This method can be used to classify chemicals when only a few properties are known and can be used to direct further data collection. Case studies are presented to demonstrate the advantages of the approach.

  19. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    SciTech Connect (OSTI)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This also represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.

  20. Binding of undamaged double stranded DNA to vaccinia virus uracil-DNA glycosylase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Schormann, Norbert; Banerjee, Surajit; Ricciardi, Robert; Chattopadhyay, Debasish

    2015-06-02

    Background: Uracil-DNA glycosylases are evolutionarily conserved DNA repair enzymes. However, vaccinia virus uracil-DNA glycosylase (known as D4), also serves as an intrinsic and essential component of the processive DNA polymerase complex during DNA replication. In this complex D4 binds to a unique poxvirus specific protein A20 which tethers it to the DNA polymerase. At the replication fork the DNA scanning and repair function of D4 is coupled with DNA replication. So far, DNA-binding to D4 has not been structurally characterized. Results: This manuscript describes the first structure of a DNA-complex of a uracil-DNA glycosylase from the poxvirus family. This alsomore » represents the first structure of a uracil DNA glycosylase in complex with an undamaged DNA. In the asymmetric unit two D4 subunits bind simultaneously to complementary strands of the DNA double helix. Each D4 subunit interacts mainly with the central region of one strand. DNA binds to the opposite side of the A20-binding surface on D4. In comparison of the present structure with the structure of uracil-containing DNA-bound human uracil-DNA glycosylase suggests that for DNA binding and uracil removal D4 employs a unique set of residues and motifs that are highly conserved within the poxvirus family but different in other organisms. Conclusion: The first structure of D4 bound to a truly non-specific undamaged double-stranded DNA suggests that initial binding of DNA may involve multiple non-specific interactions between the protein and the phosphate backbone.« less

  1. Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase

    SciTech Connect (OSTI)

    Tessier, D.C.; Brousseau, R.; Vernet, T.

    1986-10-01

    Despite its unique ability to ligate single-stranded DNA molecules, T4 RNA ligase has so far seen little use in molecular biology due to long reaction times, modest yields, and apparent inability to promote ligations of long oligodeoxyribonucleotides. The authors describe here a set of reaction conditions which dramatically shorten the reaction time and give reproducible 40 to 60% ligation of DNA fragments of up to 40 bases in length. These improvements open promising new fields of application to T4 RNA ligase.

  2. Communications circuit including a linear quadratic estimator

    DOE Patents [OSTI]

    Ferguson, Dennis D.

    2015-07-07

    A circuit includes a linear quadratic estimator (LQE) configured to receive a plurality of measurements a signal. The LQE is configured to weight the measurements based on their respective uncertainties to produce weighted averages. The circuit further includes a controller coupled to the LQE and configured to selectively adjust at least one data link parameter associated with a communication channel in response to receiving the weighted averages.

  3. Gas storage materials, including hydrogen storage materials

    DOE Patents [OSTI]

    Mohtadi, Rana F; Wicks, George G; Heung, Leung K; Nakamura, Kenji

    2013-02-19

    A material for the storage and release of gases comprises a plurality of hollow elements, each hollow element comprising a porous wall enclosing an interior cavity, the interior cavity including structures of a solid-state storage material. In particular examples, the storage material is a hydrogen storage material such as a solid state hydride. An improved method for forming such materials includes the solution diffusion of a storage material solution through a porous wall of a hollow element into an interior cavity.

  4. Intentionally Including - Engaging Minorities in Physics Careers |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Intentionally Including - Engaging Minorities in Physics Careers Intentionally Including - Engaging Minorities in Physics Careers April 24, 2013 - 4:37pm Addthis Joining Director Dot Harris (second from left) were Marlene Kaplan, the Deputy Director of Education and director of EPP, National Oceanic and Atmospheric Administration, Claudia Rankins, a Program Officer with the National Science Foundation and Jim Stith, the past Vice-President of the American Institute of

  5. Gas storage materials, including hydrogen storage materials

    DOE Patents [OSTI]

    Mohtadi, Rana F; Wicks, George G; Heung, Leung K; Nakamura, Kenji

    2014-11-25

    A material for the storage and release of gases comprises a plurality of hollow elements, each hollow element comprising a porous wall enclosing an interior cavity, the interior cavity including structures of a solid-state storage material. In particular examples, the storage material is a hydrogen storage material, such as a solid state hydride. An improved method for forming such materials includes the solution diffusion of a storage material solution through a porous wall of a hollow element into an interior cavity.

  6. Persistent current in the ferromagnetic Kondo lattice model

    SciTech Connect (OSTI)

    Zang, J.; Trugman, S.A.; Bishop, A.R.; Roeder, H.

    1997-11-01

    In this paper, we study the zero-temperature persistent current in a ferromagnetic Kondo lattice model in the strong coupling limit. In this model, there are spontaneous spin textures at some values of the external magnetic flux. These spin textures contribute a geometric flux, which can induce an additional spontaneous persistent current. Since this spin texture changes with the external magnetic flux, we find that there is an anomalous persistent current in some region of magnetic flux: near {Phi}/{Phi}{sub 0}=0 for an even number of electrons and {Phi}/{Phi}{sub 0}=1/2 for an odd number of electrons. {copyright} {ital 1997} {ital The American Physical Society}

  7. Scramjet including integrated inlet and combustor

    SciTech Connect (OSTI)

    Kutschenreuter, P.H. Jr.; Blanton, J.C.

    1992-02-04

    This patent describes a scramjet engine. It comprises: a first surface including an aft facing step; a cowl including: a leading edge and a trailing edge; an upper surface and a lower surface extending between the leading edge and the trailing edge; the cowl upper surface being spaced from and generally parallel to the first surface to define an integrated inlet-combustor therebetween having an inlet for receiving and channeling into the inlet-combustor supersonic inlet airflow; means for injecting fuel into the inlet-combustor at the step for mixing with the supersonic inlet airflow for generating supersonic combustion gases; and further including a spaced pari of sidewalls extending between the first surface to the cowl upper surface and wherein the integrated inlet-combustor is generally rectangular and defined by the sidewall pair, the first surface and the cowl upper surface.

  8. Electric Power Monthly, August 1990. [Glossary included

    SciTech Connect (OSTI)

    Not Available

    1990-11-29

    The Electric Power Monthly (EPM) presents monthly summaries of electric utility statistics at the national, Census division, and State level. The purpose of this publication is to provide energy decisionmakers with accurate and timely information that may be used in forming various perspectives on electric issues that lie ahead. Data includes generation by energy source (coal, oil, gas, hydroelectric, and nuclear); generation by region; consumption of fossil fuels for power generation; sales of electric power, cost data; and unusual occurrences. A glossary is included.

  9. Photoactive devices including porphyrinoids with coordinating additives

    DOE Patents [OSTI]

    Forrest, Stephen R; Zimmerman, Jeramy; Yu, Eric K; Thompson, Mark E; Trinh, Cong; Whited, Matthew; Diev, Vlacheslav

    2015-05-12

    Coordinating additives are included in porphyrinoid-based materials to promote intermolecular organization and improve one or more photoelectric characteristics of the materials. The coordinating additives are selected from fullerene compounds and organic compounds having free electron pairs. Combinations of different coordinating additives can be used to tailor the characteristic properties of such porphyrinoid-based materials, including porphyrin oligomers. Bidentate ligands are one type of coordinating additive that can form coordination bonds with a central metal ion of two different porphyrinoid compounds to promote porphyrinoid alignment and/or pi-stacking. The coordinating additives can shift the absorption spectrum of a photoactive material toward higher wavelengths, increase the external quantum efficiency of the material, or both.

  10. Subterranean barriers including at least one weld

    DOE Patents [OSTI]

    Nickelson, Reva A.; Sloan, Paul A.; Richardson, John G.; Walsh, Stephanie; Kostelnik, Kevin M.

    2007-01-09

    A subterranean barrier and method for forming same are disclosed, the barrier including a plurality of casing strings wherein at least one casing string of the plurality of casing strings may be affixed to at least another adjacent casing string of the plurality of casing strings through at least one weld, at least one adhesive joint, or both. A method and system for nondestructively inspecting a subterranean barrier is disclosed. For instance, a radiographic signal may be emitted from within a casing string toward an adjacent casing string and the radiographic signal may be detected from within the adjacent casing string. A method of repairing a barrier including removing at least a portion of a casing string and welding a repair element within the casing string is disclosed. A method of selectively heating at least one casing string forming at least a portion of a subterranean barrier is disclosed.

  11. Electric power monthly, September 1990. [Glossary included

    SciTech Connect (OSTI)

    Not Available

    1990-12-17

    The purpose of this report is to provide energy decision makers with accurate and timely information that may be used in forming various perspectives on electric issues. The power plants considered include coal, petroleum, natural gas, hydroelectric, and nuclear power plants. Data are presented for power generation, fuel consumption, fuel receipts and cost, sales of electricity, and unusual occurrences at power plants. Data are compared at the national, Census division, and state levels. 4 figs., 52 tabs. (CK)

  12. Power generation method including membrane separation

    DOE Patents [OSTI]

    Lokhandwala, Kaaeid A.

    2000-01-01

    A method for generating electric power, such as at, or close to, natural gas fields. The method includes conditioning natural gas containing C.sub.3+ hydrocarbons and/or acid gas by means of a membrane separation step. This step creates a leaner, sweeter, drier gas, which is then used as combustion fuel to run a turbine, which is in turn used for power generation.

  13. Nuclear reactor shield including magnesium oxide

    DOE Patents [OSTI]

    Rouse, Carl A.; Simnad, Massoud T.

    1981-01-01

    An improvement in nuclear reactor shielding of a type used in reactor applications involving significant amounts of fast neutron flux, the reactor shielding including means providing structural support, neutron moderator material, neutron absorber material and other components as described below, wherein at least a portion of the neutron moderator material is magnesium in the form of magnesium oxide either alone or in combination with other moderator materials such as graphite and iron.

  14. Rotor assembly including superconducting magnetic coil

    DOE Patents [OSTI]

    Snitchler, Gregory L.; Gamble, Bruce B.; Voccio, John P.

    2003-01-01

    Superconducting coils and methods of manufacture include a superconductor tape wound concentrically about and disposed along an axis of the coil to define an opening having a dimension which gradually decreases, in the direction along the axis, from a first end to a second end of the coil. Each turn of the superconductor tape has a broad surface maintained substantially parallel to the axis of the coil.

  15. Segregation of DNA polynucleotide strands into sister chromatids and the use of endoreduplicated cells to track sister chromatid exchanges induced by crosslinks, alkylations, or x-ray damage

    SciTech Connect (OSTI)

    Wolff, S.; Afzal, V.

    1996-06-11

    The method of Matsumoto and Ohta to induce large numbers of endoreduplicated Chinese hamster ovary cells has now been coupled with the fluorescence-plus-Giemsa method of Perry and Wolff to produce harlequin endoreduplicated chromosomes that after the third round of DNA replication are composed of a chromosome with a light chromatid and a dark chromatid in close apposition to its sister chromosome containing two light chromatids. Unless the pattern is disrupted by sister chromatid exchange (SCE), the dark chromatid is always in the center, so that the order of the chromatids is light-dark light-light. The advent of this method, which permits the observation of SCEs in endoreduplicated cells, makes it possible to determine with great ease in which cell cycle an SCE occurred. This now allows us to approach several vexing questions about the induction of SCEs (genetic damage and its repair) after exposure to various types of mutagenic carcinogens. The present experiments have allowed observation of how many cell cycles various types of lesions that are induced in DNA by a crosslinking agent, an alkylating agent, or ionizing radiation, and that are responsible for the induction of SCEs, persist before being repaired and thus lose their ability to inflict genetic damage. Other experiments with various types of mutagenic carcinogens and various types of cell lines that have defects in different DNA repair processes, such as mismatch repair, excision repair, crosslink repair, and DNA-strand-break repair, can now be carried out to determine the role of these types of repair in removing specific types of lesions. 22 refs., 3 figs., 1 tab.

  16. Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

    SciTech Connect (OSTI)

    Ibanez, Irene L.; Bracalente, Candelaria; Molinari, Beatriz L.; Palmieri, Monica A.; Policastro, Lucia; Kreiner, Andres J.; Burlon, Alejandro A.; Valda, Alejandro; Navalesi, Daniela; Davidson, Jorge; Davidson, Miguel; Vazquez, Monica; Ozafran, Mabel; Duran, Hebe

    2009-07-15

    Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX ({gamma}H2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. {gamma}H2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of {gamma}H2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of {gamma}H2AX foci. We conclude that {gamma}H2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.

  17. Optical panel system including stackable waveguides

    DOE Patents [OSTI]

    DeSanto, Leonard; Veligdan, James T.

    2007-03-06

    An optical panel system including stackable waveguides is provided. The optical panel system displays a projected light image and comprises a plurality of planar optical waveguides in a stacked state. The optical panel system further comprises a support system that aligns and supports the waveguides in the stacked state. In one embodiment, the support system comprises at least one rod, wherein each waveguide contains at least one hole, and wherein each rod is positioned through a corresponding hole in each waveguide. In another embodiment, the support system comprises at least two opposing edge structures having the waveguides positioned therebetween, wherein each opposing edge structure contains a mating surface, wherein opposite edges of each waveguide contain mating surfaces which are complementary to the mating surfaces of the opposing edge structures, and wherein each mating surface of the opposing edge structures engages a corresponding complementary mating surface of the opposite edges of each waveguide.

  18. Optical panel system including stackable waveguides

    DOE Patents [OSTI]

    DeSanto, Leonard; Veligdan, James T.

    2007-11-20

    An optical panel system including stackable waveguides is provided. The optical panel system displays a projected light image and comprises a plurality of planar optical waveguides in a stacked state. The optical panel system further comprises a support system that aligns and supports the waveguides in the stacked state. In one embodiment, the support system comprises at least one rod, wherein each waveguide contains at least one hole, and wherein each rod is positioned through a corresponding hole in each waveguide. In another embodiment, the support system comprises at least two opposing edge structures having the waveguides positioned therebetween, wherein each opposing edge structure contains a mating surface, wherein opposite edges of each waveguide contain mating surfaces which are complementary to the mating surfaces of the opposing edge structures, and wherein each mating surface of the opposing edge structures engages a corresponding complementary mating surface of the opposite edges of each waveguide.

  19. Thermovoltaic semiconductor device including a plasma filter

    DOE Patents [OSTI]

    Baldasaro, Paul F.

    1999-01-01

    A thermovoltaic energy conversion device and related method for converting thermal energy into an electrical potential. An interference filter is provided on a semiconductor thermovoltaic cell to pre-filter black body radiation. The semiconductor thermovoltaic cell includes a P/N junction supported on a substrate which converts incident thermal energy below the semiconductor junction band gap into electrical potential. The semiconductor substrate is doped to provide a plasma filter which reflects back energy having a wavelength which is above the band gap and which is ineffectively filtered by the interference filter, through the P/N junction to the source of radiation thereby avoiding parasitic absorption of the unusable portion of the thermal radiation energy.

  20. Drapery assembly including insulated drapery liner

    DOE Patents [OSTI]

    Cukierski, Gwendolyn (Ithaca, NY)

    1983-01-01

    A drapery assembly is disclosed for covering a framed wall opening, the assembly including drapery panels hung on a horizontal traverse rod, the rod having a pair of master slides and means for displacing the master slides between open and closed positions. A pair of insulating liner panels are positioned behind the drapery, the remote side edges of the liner panels being connected with the side portions of the opening frame, and the adjacent side edges of the liner panels being connected with a pair of vertically arranged center support members adapted for sliding movement longitudinally of a horizontal track member secured to the upper horizontal portion of the opening frame. Pivotally arranged brackets connect the center support members with the master slides of the traverse rod whereby movement of the master slides to effect opening and closing of the drapery panels effects simultaneous opening and closing of the liner panels.

  1. Proximity-induced superconductivity effect in a double-stranded DNA

    SciTech Connect (OSTI)

    Simchi, Hamidreza; Esmaeilzadeh, Mahdi Mazidabadi, Hossein

    2014-02-07

    We study the proximity-induced superconductivity effect in a double-stranded DNA by solving the Bogoliubov-de Gennes equations and taking into account the effect of thermal fluctuations of the twist angle between neighboring base pairs. We show that the electron conductance is spin-dependent and the conductance of spin up (down) increases (decreases) due to the spin-orbit coupling (SOC). It is found that, for T < 100 K, the band gap energy is temperature-independent and it decreases due to the SOC. In addition, by solving the Bogoliubov-de Gennes equations and local gap parameter equation self-consistently, we find the critical temperature at which transition to superconductivity can take place.

  2. Double-stranded DNA organization in bacteriophage heads: An alternative toroid-based model

    SciTech Connect (OSTI)

    Hud, N.V.

    1995-10-01

    Studies of the organization of double-stranded DNA within bacteriophage heads during the past four decades have produced a wealth of data. However, despite the presentation of numerous models, the true organization of DNA within phage heads remains unresolved. The observations of toroidal DNA structures in electron micrographs of phage lysates have long been cited as support for the organization of DNA in a spool-like fashion. This particular model, like all other models, has not been found to be consistent with all available data. Recently, the authors proposed that DNA within toroidal condensates produced in vitro is organized in a manner significantly different from that suggested by the spool model. This new toroid model has allowed the development of an alternative model for DNA organization within bacteriophage heads that is consistent with a wide range of biophysical data. Here the authors propose that bacteriophage DNA is packaged in a toroid that is folded into a highly compact structure.

  3. Crystal Structure of a Super Leucine Zipper an Extended Two-Stranded Super Long Coiled Coil

    SciTech Connect (OSTI)

    J Diao

    2011-12-31

    Coiled coil is a ubiquitous structural motif in proteins, with two to seven alpha helices coiled together like the strands of a rope, and coiled coil folding and assembly is not completely understood. A GCN4 leucine zipper mutant with four mutations of K3A, D7A, Y17W, and H18N has been designed, and the crystal structure has been determined at 1.6 {angstrom} resolution. The peptide monomer shows a helix trunk with short curved N- and C-termini. In the crystal, two monomers cross in 35{sup o} and form an X-shaped dimer, and each X-shaped dimer is welded into the next one through sticky hydrophobic ends, thus forming an extended two-stranded, parallel, super long coiled coil rather than a discrete, two-helix coiled coil of the wild-type GCN4 leucine zipper. Leucine residues appear at every seventh position in the super long coiled coil, suggesting that it is an extended super leucine zipper. Compared to the wild-type leucine zipper, the N-terminus of the mutant has a dramatic conformational change and the C-terminus has one more residue Glu 32 determined. The mutant X-shaped dimer has a large crossing angle of 35{sup o} instead of 18{sup o} in the wild-type dimer. The results show a novel assembly mode and oligomeric state of coiled coil, and demonstrate that mutations may affect folding and assembly of the overall coiled coil. Analysis of the formation mechanism of the super long coiled coil may help understand and design self-assembling protein fibers.

  4. Articles including thin film monolayers and multilayers

    DOE Patents [OSTI]

    Li, DeQuan; Swanson, Basil I.

    1995-01-01

    Articles of manufacture including: (a) a base substrate having an oxide surface layer, and a multidentate ligand, capable of binding a metal ion, attached to the oxide surface layer of the base substrate, (b) a base substrate having an oxide surface layer, a multidentate ligand, capable of binding a metal ion, attached to the oxide surface layer of the base substrate, and a metal species attached to the multidentate ligand, (c) a base substrate having an oxide surface layer, a multidentate ligand, capable of binding a metal ion, attached to the oxide surface layer of the base substrate, a metal species attached to the multidentate ligand, and a multifunctional organic ligand attached to the metal species, and (d) a base substrate having an oxide surface layer, a multidentate ligand, capable of binding a metal ion, attached to the oxide surface layer of the base substrate, a metal species attached to the multidentate ligand, a multifunctional organic ligand attached to the metal species, and a second metal species attached to the multifunctional organic ligand, are provided, such articles useful in detecting the presence of a selected target species, as nonliear optical materials, or as scavengers for selected target species.

  5. Engine lubrication circuit including two pumps

    DOE Patents [OSTI]

    Lane, William H.

    2006-10-03

    A lubrication pump coupled to the engine is sized such that the it can supply the engine with a predetermined flow volume as soon as the engine reaches a peak torque engine speed. In engines that operate predominately at speeds above the peak torque engine speed, the lubrication pump is often producing lubrication fluid in excess of the predetermined flow volume that is bypassed back to a lubrication fluid source. This arguably results in wasted power. In order to more efficiently lubricate an engine, a lubrication circuit includes a lubrication pump and a variable delivery pump. The lubrication pump is operably coupled to the engine, and the variable delivery pump is in communication with a pump output controller that is operable to vary a lubrication fluid output from the variable delivery pump as a function of at least one of engine speed and lubrication flow volume or system pressure. Thus, the lubrication pump can be sized to produce the predetermined flow volume at a speed range at which the engine predominately operates while the variable delivery pump can supplement lubrication fluid delivery from the lubrication pump at engine speeds below the predominant engine speed range.

  6. Persistence of uranium emission in laser-produced plasmas

    SciTech Connect (OSTI)

    LaHaye, N. L.; Harilal, S. S. Diwakar, P. K.; Hassanein, A.

    2014-04-28

    Detection of uranium and other nuclear materials is of the utmost importance for nuclear safeguards and security. Optical emission spectroscopy of laser-ablated U plasmas has been presented as a stand-off, portable analytical method that can yield accurate qualitative and quantitative elemental analysis of a variety of samples. In this study, optimal laser ablation and ambient conditions are explored, as well as the spatio-temporal evolution of the plasma for spectral analysis of excited U species in a glass matrix. Various Ar pressures were explored to investigate the role that plasma collisional effects and confinement have on spectral line emission enhancement and persistence. The plasma-ambient gas interaction was also investigated using spatially resolved spectra and optical time-of-flight measurements. The results indicate that ambient conditions play a very important role in spectral emission intensity as well as the persistence of excited neutral U emission lines, influencing the appropriate spectral acquisition conditions.

  7. Graphene: Amazing Material Found Thanks to Scotch Tape and Persistent

    Office of Science (SC) Website

    Science | U.S. DOE Office of Science (SC) 1 » Graphene: Amazing Material Found Thanks to Scotch Tape and Persistent Science News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science Highlights Presentations & Testimony News Archives Communications and Public Affairs Contact Information Office of Science U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 P: (202) 586-5430 03.25.11 Graphene: Amazing

  8. Persisting cold extremes under 21st-century warming scenarios

    SciTech Connect (OSTI)

    Kodra, Evan A; Steinhaeuser, Karsten J K; Ganguly, Auroop R

    2011-01-01

    Analyses of climate model simulations and observations reveal that extreme cold events are likely to persist across each land-continent even under 21st-century warming scenarios. The grid-based intensity, duration and frequency of cold extreme events are calculated annually through three indices: the coldest annual consecutive three-day average of daily maximum temperature, the annual maximum of consecutive frost days, and the total number of frost days. Nine global climate models forced with a moderate greenhouse-gas emissions scenario compares the indices over 2091 2100 versus 1991 2000. The credibility of model-simulated cold extremes is evaluated through both bias scores relative to reanalysis data in the past and multi-model agreement in the future. The number of times the value of each annual index in 2091 2100 exceeds the decadal average of the corresponding index in 1991 2000 is counted. The results indicate that intensity and duration of grid-based cold extremes, when viewed as a global total, will often be as severe as current typical conditions in many regions, but the corresponding frequency does not show this persistence. While the models agree on the projected persistence of cold extremes in terms of global counts, regionally, inter-model variability and disparity in model performance tends to dominate. Our findings suggest that, despite a general warming trend, regional preparedness for extreme cold events cannot be compromised even towards the end of the century.

  9. Ionic liquids, electrolyte solutions including the ionic liquids, and energy storage devices including the ionic liquids

    SciTech Connect (OSTI)

    Gering, Kevin L.; Harrup, Mason K.; Rollins, Harry W.

    2015-12-08

    An ionic liquid including a phosphazene compound that has a plurality of phosphorus-nitrogen units and at least one pendant group bonded to each phosphorus atom of the plurality of phosphorus-nitrogen units. One pendant group of the at least one pendant group comprises a positively charged pendant group. Additional embodiments of ionic liquids are disclosed, as are electrolyte solutions and energy storage devices including the embodiments of the ionic liquid.

  10. Parallel Computation of Persistent Homology using the Blowup Complex

    SciTech Connect (OSTI)

    Lewis, Ryan; Morozov, Dmitriy

    2015-04-27

    We describe a parallel algorithm that computes persistent homology, an algebraic descriptor of a filtered topological space. Our algorithm is distinguished by operating on a spatial decomposition of the domain, as opposed to a decomposition with respect to the filtration. We rely on a classical construction, called the Mayer--Vietoris blowup complex, to glue global topological information about a space from its disjoint subsets. We introduce an efficient algorithm to perform this gluing operation, which may be of independent interest, and describe how to process the domain hierarchically. We report on a set of experiments that help assess the strengths and identify the limitations of our method.

  11. Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

    SciTech Connect (OSTI)

    Muraguchi, Hajime; Umezawa, Kiwamu; Niikura, Mai; Yoshida, Makoto; Kozaki, Toshinori; Ishii, Kazuo; Sakai, Kiyota; Shimizu, Motoyuki; Nakahori, Kiyoshi; Sakamoto, Yuichi; Choi, Cindy; Ngan, Chew Yee; Lindquist, Eika; Lipzen, Anna; Tritt, Andrew; Haridas, Sajeet; Barry, Kerrie; Grigoriev, Igor V.; Pukkila, Patricia J.

    2015-10-28

    We report that the basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC). To validate RNA-seq data, expression levels of selected genes were compared using RPKM values in RNA-seq data and qRT-PCR data, and DEGs detected in microarray data were examined in MA plots of RNA-seq data by TCC. We discuss events deduced from GO analysis of DEGs. In addition, we uncovered both transcription factor candidates and antisense transcripts that are likely to be involved in developmental regulation for fruiting.

  12. Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy

    SciTech Connect (OSTI)

    Xiao, Xiao; Gang, Yi; Wang, Honghong; Wang, Jiayin; Zhao, Lina; Xu, Li; Liu, Zhiguo

    2015-02-06

    Highlights: • A shRNA vector based transcription factor decoy, VB-ODN, was designed. • VB-ODN for NF-κB inhibited cell viability in HEK293 cells. • VB-ODN inhibited expression of downstream genes of target transcription factors. • VB-ODN may enhance nuclear entry ratio for its feasibility of virus production. - Abstract: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.

  13. Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Muraguchi, Hajime; Umezawa, Kiwamu; Niikura, Mai; Yoshida, Makoto; Kozaki, Toshinori; Ishii, Kazuo; Sakai, Kiyota; Shimizu, Motoyuki; Nakahori, Kiyoshi; Sakamoto, Yuichi; et al

    2015-10-28

    We report that the basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq) was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues) with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs) between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC).more » To validate RNA-seq data, expression levels of selected genes were compared using RPKM values in RNA-seq data and qRT-PCR data, and DEGs detected in microarray data were examined in MA plots of RNA-seq data by TCC. We discuss events deduced from GO analysis of DEGs. In addition, we uncovered both transcription factor candidates and antisense transcripts that are likely to be involved in developmental regulation for fruiting.« less

  14. Laminar burn rates of gun propellants measured in the high-pressure strand burner

    SciTech Connect (OSTI)

    Reaugh, J. E., LLNL

    1997-10-01

    The pressure dependence of the laminar burn rate of gun propellants plays a role in the design and behavior of high-performance guns. We have begun a program to investigate the effects of processing variables on the laminar burn rates, using our high-pressure strand burner to measure these rates at pressures exceeding 700 MPa. We have burned JA2 and M43 propellant samples, provided by Dr. Arpad Juhasz, ARL, from propellant lots previously used in round-robin tests. Our results at room temperature are in accord with other measurements. In addition, we present results measured for propellant that has been preheated to 50 C before burning. We used our thermochemical equilibrium code, CHEETAH, to help interpret the simultaneous pressure and temperature measurements taken during the testing, and show examples of its use. It has been modified to provide performance measures and equations of state for the products that are familiar to the gun-propellant community users of BLAKE.

  15. Induction and Persistence of Large ?H2AX Foci by High Linear Energy Transfer Radiation in DNA-Dependent protein kinaseDeficient Cells

    SciTech Connect (OSTI)

    Bracalente, Candelaria; Ibaez, Irene L.; Molinari, Beatriz; Palmieri, Mnica; Kreiner, Andrs; Valda, Alejandro; and others

    2013-11-15

    Purpose: To evaluate the cell response to DNA double-strand breaks induced by low and high linear energy transfer (LET) radiations when the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), an essential protein of the nonhomologous end-joining repair pathway, lacks kinase activity. Methods and Materials: CHO10B2, a Chinese hamster ovary cell line, and its derived radiosensitive mutant cell line, irs-20, lacking DNA-PKcs activity, were evaluated after 0 to 3 Gy of ?-rays, plateau and Bragg peak protons, and lithium beams by clonogenic assay, and as a measurement of double-strand breaks, phosphorylated H2AX (?H2AX) foci number and size were quantified by immunocytofluorescence. Results: Irs-20 exhibited greater radiosensitivity and a higher amount of ?H2AX foci than CHO10B2 at 6 hours after irradiation for all types of radiations. Remarkably, CHO10B2 and irs-20 maintained their difference in radiosensitivity after high-LET radiation. Six hours after low-LET radiations, irs-20 did not reach basal levels of ?H2AX at high doses, whereas CHO10B2 recovered basal levels for all doses. After high-LET radiation, only CHO10B2 exhibited a reduction in ?H2AX foci, but it never reached basal levels. Persistent foci in irs-20 confirmed a repair deficiency. Interestingly, after 30 minutes of high-LET radiation both cell lines exhibited large foci (size >0.9 ?m{sup 2}) related to the damage nature, whereas at 6 hours irs-20 showed a higher amount of large foci than CHO10B2, with a 7-fold increase at 3 Gy, that could also be associated to radiosensitivity. Conclusions: We demonstrated, for the first time, an association between deficient DNA-PKcs activity and not only high levels of H2AX phosphorylation but also persistence and size increase of ?H2AX foci after high-LET irradiation.

  16. The Promiscuity of [beta]-Strand Pairing Allows for Rational Design of [beta]-Sheet Face Inversion

    SciTech Connect (OSTI)

    Makabe, Koki; Koide, Shohei

    2009-06-17

    Recent studies suggest the dominant role of main-chain H-bond formation in specifying {beta}-sheet topology. Its essentially sequence-independent nature implies a large degree of freedom in designing {beta}-sheet-based nanomaterials. Here we show rational design of {beta}-sheet face inversions by incremental deletions of {beta}-strands from the single-layer {beta}-sheet of Borrelia outer surface protein A. We show that a {beta}-sheet structure can be maintained when a large number of native contacts are removed and that one can design large-scale conformational transitions of a {beta}-sheet such as face inversion by exploiting the promiscuity of strand-strand interactions. High-resolution X-ray crystal structures confirmed the success of the design and supported the importance of main-chain H-bonds in determining {beta}-sheet topology. This work suggests a simple but effective strategy for designing and controlling nanomaterials based on {beta}-rich peptide self-assemblies.

  17. Activation of double-stranded RNA-dependent protein kinase inhibits proliferation of pancreatic ?-cells

    SciTech Connect (OSTI)

    Chen, Shan-Shan [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Jiang, Teng [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Wang, Yi; Gu, Li-Ze [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China); Wu, Hui-Wen [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China)] [Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing (China); Tan, Lan [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China)] [Department of Neurology, Qingdao Municipal Hospital, Nanjing Medical University, Nanjing (China); Guo, Jun, E-mail: Guoj@njmu.edu.cn [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China) [Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing (China); Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing (China)

    2014-01-17

    Highlights: PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in ?-cells. Activated PKR inhibited ?-cell proliferation by arresting cell cycle at G1 phase. Activated PKR fully abrogated the pro-proliferative effects of IGF-I on ?-cells. -- Abstract: Double-stranded RNA-dependent protein kinase (PKR) is revealed to participate in the development of insulin resistance in peripheral tissues in type 2 diabetes (T2DM). Meanwhile, PKR is also characterized as a critical regulator of cell proliferation. To date, no study has focused on the impact of PKR on the proliferation of pancreatic ?-cells. Here, we adopted insulinoma cell lines and mice islet ?-cells to investigate: (1) the effects of glucolipotoxicity and pro-inflammatory cytokines on PKR activation; (2) the effects of PKR on proliferation of pancreatic ?-cells and its underlying mechanisms; (3) the actions of PKR on pro-proliferative effects of IGF-I and its underlying pathway. Our results provided the first evidence that PKR can be activated by glucolipitoxicity and pro-inflammatory cytokines in pancreatic ?-cells, and activated PKR significantly inhibited cell proliferation by arresting cell cycle at G1 phase. Reductions in cyclin D1 and D2 as well as increases in p27 and p53 were associated with the anti-proliferative effects of PKR, and proteasome-dependent degradation took part in the reduction of cyclin D1 and D2. Besides, PKR activation abrogated the pro-proliferative effects of IGF-I by activating JNK and disrupting IRS1/PI3K/Akt signaling pathway. These findings indicate that the anti-proliferative actions of PKR on pancreatic ?-cells may contribute to the pathogenesis of T2DM.

  18. Eastern Seaboard Electric Grid Fragility Maps Supporting Persistent Availability

    SciTech Connect (OSTI)

    Walker, Kimberly A; Weigand, Gilbert G; Fernandez, Steven J

    2012-11-01

    Persistently available power transmission can be disrupted by weather causing power outages with economic and social consequences. This research investigated the effects on the national power grid from a specific weather event, Hurricane Irene, that caused approximately 5.7 million customer power outages along the Eastern Seaboard in August of 2011. The objective was to describe the geographic differences in the grid s vulnerability to these events. Individual factors, such as wind speed or precipitation, were correlated with the number of outages to determine the greatest mechanism of power failure in hopes of strengthening the future power grid. The resulting fragility maps not only depicted 18 counties that were less robust than the design-standard robustness model and three counties that were more robust, but also drew new damage contours with correlated wind speeds and county features.

  19. Titanium subhydride potassium perchlorate (TiH1.65/KClO4) burn rates from hybrid closed bomb-strand burner experiments.

    SciTech Connect (OSTI)

    Cooper, Marcia A.; Oliver, Michael S.

    2012-08-01

    A hybrid closed bomb-strand burner is used to measure the burning behavior of the titanium subhydride potassium perchlorate pyrotechnic with an equivalent hydrogen concentration of 1.65. This experimental facility allows for simultaneous measurement of the closed bomb pressure rise and pyrotechnic burn rate as detected by electrical break wires over a range of pressures. Strands were formed by pressing the pyrotechnic powders to bulk densities between 60% and 90% theoretical maximum density. The burn rate dependance on initial density and vessel pressure are measured. At all initial strand densities, the burn is observed to transition from conductive to convective burning within the strand. The measured vessel pressure history is further analyzed following the closed bomb analysis methods developed for solid propellants.

  20. Paul Bohn, Three Persistent Challenges | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Paul Bohn, Three Persistent Challenges Chemical Sciences, Geosciences, & Biosciences (CSGB) Division CSGB Home About Research Areas Reports and Activities Science Highlights Principal Investigators' Meetings BES Home Third DOE BES Separations Research Workshop Paul Bohn, Three Persistent Challenges Print Text Size: A A A FeedbackShare Page Third DOE/Basic Energy Sciences Separations Research Workshop Savannah DeSoto Hilton, Savannah, Georgia May 12-14, 1999 Three Persistent Challenges Paul

  1. Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing through Partnerships

    Broader source: Energy.gov [DOE]

    This presentation, given through the DOE's Technical Assitance Program (TAP), provides information on Overcoming Persistent Barriers to Energy Efficiency in Multifamily Housing through Partnerships.

  2. Persistent Fe moments in the normal state of the pressure-induced...

    Office of Scientific and Technical Information (OSTI)

    Persistent Fe moments in the normal state of the pressure-induced superconductor ... Resource Relation: Journal Name: Physical Review B, vol. 90, no. 14, October 13, 2014, pp. ...

  3. Evaluation of Juvenile Fall Chinook Salmon Stranding on the Hanford Reach in the Columbia River, 1998 Interim Report.

    SciTech Connect (OSTI)

    Nugent, John; Newsome, Todd; Nugent, Michael

    2001-07-27

    The Washington Department of Fish and Wildlife (WDFW) has been contracted through the Bonneville Power Administration (BPA) and the Grant County Public Utility District (GCPUD) to perform an evaluation of juvenile fall chinook salmon (Oncorhynchus tshawytscha) stranding on the Hanford Reach. The evaluation, in the second year of a multi-year study, has been developed to assess the impacts of water fluctuations from Priest Rapids Dam on rearing juvenile fall chinook salmon, other fish species, and benthic macroinvertebrates of the Hanford Reach. This document provides the results of the 1998 field season.

  4. Evaluation of Juvenile Fall Chinook Salmon Stranding on the Hanford Reach of the Columbia River, 1999 Annual Report.

    SciTech Connect (OSTI)

    Nugent, John

    2002-01-24

    The Washington Department of Fish and Wildlife (WDFW) has been contracted through the Bonneville Power Administration (BPA) and the Grant County Public Utility District (GCPUD) to perform an evaluation of juvenile fall chinook salmon (Oncorhynchus tshawytscha) stranding on the Hanford Reach. The evaluation, in the third year of a multi-year study, has been developed to assess the impacts of water fluctuations from Priest Rapids Dam on rearing juvenile fall chinook salmon, other fishes, and benthic macroinvertebrates of the Hanford Reach. This document provides the results of the 1999 field season.

  5. Studies of $${\\rm Nb}_{3}{\\rm Sn}$$ Strands Based on the Restacked-Rod Process for High Field Accelerator Magnets

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Barzi, E.; Bossert, M.; Gallo, G.; Lombardo, V.; Turrioni, D.; Yamada, R.; Zlobin, A. V.

    2011-12-21

    A major thrust in Fermilab's accelerator magnet R&D program is the development of Nb3Sn wires which meet target requirements for high field magnets, such as high critical current density, low effective filament size, and the capability to withstand the cabling process. The performance of a number of strands with 150/169 restack design produced by Oxford Superconducting Technology was studied for round and deformed wires. To optimize the maximum plastic strain, finite element modeling was also used as an aid in the design. Results of mechanical, transport and metallographic analyses are presented for round and deformed wires.

  6. Evaluation of Juvenile Fall Chinook Salmon Stranding on the Hanford Reach of the Columbia River, 2000 Annual Report.

    SciTech Connect (OSTI)

    Nugent, John; Nugent, Michael; Brock, Wendy

    2002-05-29

    The Washington Department of Fish and Wildlife (WDFW) has been contracted through the Bonneville Power Administration (BPA) and the Grant County Public Utility District (GCPUD) to perform an evaluation of juvenile fall chinook salmon (Oncorhynchus tshawytscha) stranding on the Hanford Reach. The evaluation, in the fourth year of a multi-year study, has been developed to assess the impacts of water fluctuations from Priest Rapids Dam on rearing juvenile fall chinook salmon, other fishes, and benthic macroinvertebrates of the Hanford Reach. This document provides the results of the 2000 field season.

  7. Evaluation of Juvenile Fall Chinook Salmon Stranding on the Hanford Reach of the Columbia River, 2001 Annual Report.

    SciTech Connect (OSTI)

    Nugent, John; Nugent, Michael; Brock, Wendy

    2002-05-29

    The Washington Department of Fish and Wildlife (WDFW) has been contracted through the Bonneville Power Administration (BPA) and the Grant County Public Utility District (GCPUD) to perform an evaluation of juvenile fall chinook salmon (Oncorhynchus tshawytscha) stranding on the Hanford Reach of the Columbia River. The evaluation, in the fifth year of a multi-year study, has been developed to assess the impacts of water fluctuations from Priest Rapids Dam on rearing juvenile fall chinook salmon, other fishes, and benthic macroinvertebrates of the Hanford Reach. This document provides the results of the 2001 field season.

  8. Mini-Ckpts: Surviving OS Failures in Persistent Memory

    SciTech Connect (OSTI)

    Fiala, David; Mueller, Frank; Ferreira, Kurt Brian; Engelmann, Christian

    2016-01-01

    Concern is growing in the high-performance computing (HPC) community on the reliability of future extreme-scale systems. Current efforts have focused on application fault-tolerance rather than the operating system (OS), despite the fact that recent studies have suggested that failures in OS memory are more likely. The OS is critical to a system's correct and efficient operation of the node and processes it governs -- and in HPC also for any other nodes a parallelized application runs on and communicates with: Any single node failure generally forces all processes of this application to terminate due to tight communication in HPC. Therefore, the OS itself must be capable of tolerating failures. In this work, we introduce mini-ckpts, a framework which enables application survival despite the occurrence of a fatal OS failure or crash. Mini-ckpts achieves this tolerance by ensuring that the critical data describing a process is preserved in persistent memory prior to the failure. Following the failure, the OS is rejuvenated via a warm reboot and the application continues execution effectively making the failure and restart transparent. The mini-ckpts rejuvenation and recovery process is measured to take between three to six seconds and has a failure-free overhead of between 3-5% for a number of key HPC workloads. In contrast to current fault-tolerance methods, this work ensures that the operating and runtime system can continue in the presence of faults. This is a much finer-grained and dynamic method of fault-tolerance than the current, coarse-grained, application-centric methods. Handling faults at this level has the potential to greatly reduce overheads and enables mitigation of additional fault scenarios.

  9. Nuclear Arms Control R&D Consortium includes Los Alamos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear Arms Control R&D Consortium includes Los Alamos Nuclear Arms Control R&D Consortium includes Los Alamos A consortium led by the University of Michigan that includes LANL as ...

  10. Newport News in Review, ch. 47, segment includes TEDF groundbreaking...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    https:www.jlab.orgnewsarticlesnewport-news-review-ch-47-segment-includes-tedf-groundbreaking-event Newport News in Review, ch. 47, segment includes TEDF groundbreaking event...

  11. Property:Number of Plants included in Capacity Estimate | Open...

    Open Energy Info (EERE)

    Plants included in Capacity Estimate Jump to: navigation, search Property Name Number of Plants included in Capacity Estimate Property Type Number Retrieved from "http:...

  12. Property:Number of Plants Included in Planned Estimate | Open...

    Open Energy Info (EERE)

    Number of Plants Included in Planned Estimate Jump to: navigation, search Property Name Number of Plants Included in Planned Estimate Property Type String Description Number of...

  13. Solar Energy Education. Reader, Part II. Sun story. [Includes...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reader, Part II. Sun story. Includes glossary Citation Details In-Document Search Title: Solar Energy Education. Reader, Part II. Sun story. Includes glossary You are ...

  14. Natural Gas Delivered to Consumers in California (Including Vehicle...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    California (Including Vehicle Fuel) (Million Cubic Feet) Natural Gas Delivered to Consumers in California (Including Vehicle Fuel) (Million Cubic Feet) Year Jan Feb Mar Apr May Jun ...

  15. Should Title 24 Ventilation Requirements Be Amended to include...

    Office of Scientific and Technical Information (OSTI)

    include an Indoor Air Quality Procedure? Citation Details In-Document Search Title: Should Title 24 Ventilation Requirements Be Amended to include an Indoor Air Quality Procedure? ...

  16. Microfluidic devices and methods including porous polymer monoliths...

    Office of Scientific and Technical Information (OSTI)

    Microfluidic devices and methods including porous polymer monoliths Title: Microfluidic devices and methods including porous polymer monoliths Microfluidic devices and methods ...

  17. Microfluidic devices and methods including porous polymer monoliths...

    Office of Scientific and Technical Information (OSTI)

    Microfluidic devices and methods including porous polymer monoliths Citation Details In-Document Search Title: Microfluidic devices and methods including porous polymer monoliths ...

  18. Natural Gas Delivered to Consumers in Minnesota (Including Vehicle...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Minnesota (Including Vehicle Fuel) (Million Cubic Feet) Natural Gas Delivered to Consumers in Minnesota (Including Vehicle Fuel) (Million Cubic Feet) Year Jan Feb Mar Apr May Jun ...

  19. FEMP Expands ESPC ENABLE Program to Include More Energy Conservation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Expands ESPC ENABLE Program to Include More Energy Conservation Measures FEMP Expands ESPC ENABLE Program to Include More Energy Conservation Measures November 13, 2013 - 12:00am...

  20. Uranium-Bearing Evaporite Mineralization Influencing Plume Persistence...

    Office of Scientific and Technical Information (OSTI)

    In this study, "evaporite" refers to any secondary mineral precipitate that occurs due to a loss of water through evaporative processes. This includes efflorescent salt crusts, ...

  1. Persistent activation of DNA damage signaling in response to complex mixtures of PAHs in air particulate matter

    SciTech Connect (OSTI)

    Jarvis, Ian W.H.; Bergvall, Christoffer; Bottai, Matteo; Westerholm, Roger; Stenius, Ulla; Dreij, Kristian

    2013-02-01

    Complex mixtures of polycyclic aromatic hydrocarbons (PAHs) are present in air particulate matter (PM) and have been associated with many adverse human health effects including cancer and respiratory disease. However, due to their complexity, the risk of exposure to mixtures is difficult to estimate. In the present study the effects of binary mixtures of benzo[a]pyrene (BP) and dibenzo[a,l]pyrene (DBP) and complex mixtures of PAHs in urban air PM extracts on DNA damage signaling was investigated. Applying a statistical model to the data we observed a more than additive response for binary mixtures of BP and DBP on activation of DNA damage signaling. Persistent activation of checkpoint kinase 1 (Chk1) was observed at significantly lower BP equivalent concentrations in air PM extracts than BP alone. Activation of DNA damage signaling was also more persistent in air PM fractions containing PAHs with more than four aromatic rings suggesting larger PAHs contribute a greater risk to human health. Altogether our data suggests that human health risk assessment based on additivity such as toxicity equivalency factor scales may significantly underestimate the risk of exposure to complex mixtures of PAHs. The data confirms our previous findings with PAH-contaminated soil (Niziolek-Kierecka et al., 2012) and suggests a possible role for Chk1 Ser317 phosphorylation as a biological marker for future analyses of complex mixtures of PAHs. -- Highlights: ? Benzo[a]pyrene (BP), dibenzo[a,l]pyrene (DBP) and air PM PAH extracts were compared. ? Binary mixture of BP and DBP induced a more than additive DNA damage response. ? Air PM PAH extracts were more potent than toxicity equivalency factor estimates. ? Larger PAHs (> 4 rings) contribute more to the genotoxicity of PAHs in air PM. ? Chk1 is a sensitive marker for persistent activation of DNA damage signaling from PAH mixtures.

  2. Behavioral Change and Building Performance: Strategies for Significant, Persistent, and Measurable Institutional Change

    SciTech Connect (OSTI)

    Wolfe, Amy K.; Malone, Elizabeth L.; Heerwagen, Judith H.; Dion, Jerome P.

    2014-04-01

    The people who use Federal buildings — Federal employees, operations and maintenance staff, and the general public — can significantly impact a building’s environmental performance and the consumption of energy, water, and materials. Many factors influence building occupants’ use of resources (use behaviors) including work process requirements, ability to fulfill agency missions, new and possibly unfamiliar high-efficiency/high-performance building technologies; a lack of understanding, education, and training; inaccessible information or ineffective feedback mechanisms; and cultural norms and institutional rules and requirements, among others. While many strategies have been used to introduce new occupant use behaviors that promote sustainability and reduced resource consumption, few have been verified in the scientific literature or have properly documented case study results. This paper documents validated strategies that have been shown to encourage new use behaviors that can result in significant, persistent, and measureable reductions in resource consumption. From the peer-reviewed literature, the paper identifies relevant strategies for Federal facilities and commercial buildings that focus on the individual, groups of individuals (e.g., work groups), and institutions — their policies, requirements, and culture. The paper documents methods with evidence of success in changing use behaviors and enabling occupants to effectively interact with new technologies/designs. It also provides a case study of the strategies used at a Federal facility — Fort Carson, Colorado. The paper documents gaps in the current literature and approaches, and provides topics for future research.

  3. Inhibition of HAS2 induction enhances the radiosensitivity of cancer cells via persistent DNA damage

    SciTech Connect (OSTI)

    Shen, Yan Nan; Shin, Hyun-Jin; Joo, Hyun-Yoo; Park, Eun-Ran; Kim, Su-Hyeon; Hwang, Sang-Gu; Park, Sang Jun; Kim, Chun-Ho; Lee, Kee-Ho

    2014-01-17

    Highlights: •HAS2 may be a promising target for the radiosensitization of human cancer. •HAS2 is elevated (up to ∼10-fold) in irradiated radioresistant and -sensitive cancer cells. •HAS2 knockdown sensitizes cancer cells to radiation. •HAS2 knockdown potentiates irradiation-induced DNA damage and apoptotic death. •Thus, the irradiation-induced up-regulation of HAS2 contributes to the radioresistance of cancer cells. -- Abstract: Hyaluronan synthase 2 (HAS2), a synthetic enzyme for hyaluronan, regulates various aspects of cancer progression, including migration, invasion and angiogenesis. However, the possible association of HAS2 with the response of cancer cells to anticancer radiotherapy, has not yet been elucidated. Here, we show that HAS2 knockdown potentiates irradiation-induced DNA damage and apoptosis in cancer cells. Upon exposure to radiation, all of the tested human cancer cell lines exhibited marked (up to 10-fold) up-regulation of HAS2 within 24 h. Inhibition of HAS2 induction significantly reduced the survival of irradiated radioresistant and -sensitive cells. Interestingly, HAS2 depletion rendered the cells to sustain irradiation-induced DNA damage, thereby leading to an increase of apoptotic death. These findings indicate that HAS2 knockdown sensitizes cancer cells to radiation via persistent DNA damage, further suggesting that the irradiation-induced up-regulation of HAS2 contributes to the radioresistance of cancer cells. Thus, HAS2 could potentially be targeted for therapeutic interventions aimed at radiosensitizing cancer cells.

  4. SWS Online Tool now includes Multifamily Content, plus a How...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SWS Online Tool now includes Multifamily Content, plus a How-To Webinar SWS Online Tool now includes Multifamily Content, plus a How-To Webinar This announcement contains ...

  5. Natural Gas Delivered to Consumers in New Mexico (Including Vehicle...

    U.S. Energy Information Administration (EIA) Indexed Site

    Mexico (Including Vehicle Fuel) (Million Cubic Feet) Natural Gas Delivered to Consumers in New Mexico (Including Vehicle Fuel) (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul ...

  6. Quantitative Analysis of Biofuel Sustainability, Including Land Use Change

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    GHG Emissions | Department of Energy Quantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG Emissions Quantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG Emissions Plenary V: Biofuels and Sustainability: Acknowledging Challenges and Confronting Misconceptions Quantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG Emissions Jennifer B. Dunn, Energy Systems and Sustainability Analyst, Argonne National Laboratory

  7. Persistent Fe moments in the normal state of the pressure-induced...

    Office of Scientific and Technical Information (OSTI)

    the normal state of the pressure-induced superconductor Ca0.67Sr0.33Fe2As2 Citation Details In-Document Search Title: Persistent Fe moments in the normal state of the ...

  8. Activated ClpP kills persisters and eradicates a chronic biofilm infection.

    SciTech Connect (OSTI)

    Conlon, Brian P.; Nakayasu, Ernesto S.; Fleck, Laura E.; LaFleur, Michael D.; Isabella, Vincent M.; Coleman, K.; Leonard, Steve N.; Smith, Richard D.; Adkins, Joshua N.; Lewis, Kim

    2013-11-21

    The current antibiotic crisis stems from two distinct phenomena-drug resistance, and drug tolerance. Resistance mechanisms such as drug efflux or modification prevent antibiotics from binding to their targets 1, allowing pathogens to grow. Antibiotic tolerance is the property of persister cells, phenotypic variants of regular bacteria 2. Antibiotics kill by corrupting targets, but these are inactive in dormant persisters, leading to tolerance. Persisters were first identified by Joseph Bigger in 1944, when he discovered a surviving sub-population of Staphylococcus following treatment with penicillin3. Persisters are largely responsible for recalcitrance of chronic diseases such as tuberculosis, and various infections associated with biofilms - endocarditis, osteomyelitis, infections of catheters and indwelling devices, and deep-seated infections of soft tissues 4. There are a number of redundant pathways involved in persister formation5,6 precluding development of drugs inhibiting their formation. The acyldepsipeptide antibiotic (ADEP 4) has been shown to activate the ClpP protease resulting in death of growing cells 7. Here we show that ADEP4 activated ClpP becomes a fairly non-specific protease and kills persister cells by degradation of over 400 intracellular targets. clpP mutants are resistant to ADEP4 7, but we find that they display increased susceptibility to killing by a range of conventional antibiotics. Combining ADEP4 with rifampicin leads to eradication of persisters, stationary and biofilm populations of Staphylococcus aureus in vitro and in a deep-seated murine infection. Target corruption/activation provides an approach to killing persisters and eradicating chronic infections.

  9. New 500-kV Ion Source Test Strand for HIF

    SciTech Connect (OSTI)

    Sangster, T.C.; Ahle, L.E.; Halaxa, E.F.; Karpenko, V.P.; Oldaker, M.E.; Mitchell, J.W.; Beck, D.N.; Bieniosek, F.M.; Henestroza, E.; Kwan, J.W.

    2000-03-09

    One of the most challenging aspects of ion beam driven inertial fusion energy is the reliable and efficient generation of low emittance, high current ion beams. The primary ion source requirements include a rise time of order 1-{micro}sec, a pulse width of at least 20-{micro}sec, a flattop ripple of less than 0.1% and a repetition rate of at least 5-HZ. Naturally, at such a repetition rate, the duty cycle of the source must be greater than 10{sup 8} pulses. Although these specifications do not appear to exceed the state-of-the-art for pulsed power, considerable effort remains to develop a suitable high current ion source. Therefore, we are constructing a 500-kV test stand specifically for studying various ion source concepts including surface, plasma and metal vapor arc. This paper will describe the test stand design specifications as well as the details of the various subsystems and components.

  10. Structure of Rhodococcus equi virulence-associated protein B (VapB) reveals an eight-stranded antiparallel ?-barrel consisting of two Greek-key motifs

    SciTech Connect (OSTI)

    Geerds, Christina; Wohlmann, Jens; Haas, Albert; Niemann, Hartmut H.

    2014-06-18

    The structure of VapB, a member of the Vap protein family that is involved in virulence of the bacterial pathogen R. equi, was determined by SAD phasing and reveals an eight-stranded antiparallel ?-barrel similar to avidin, suggestive of a binding function. Made up of two Greek-key motifs, the topology of VapB is unusual or even unique. Members of the virulence-associated protein (Vap) family from the pathogen Rhodococcus equi regulate virulence in an unknown manner. They do not share recognizable sequence homology with any protein of known structure. VapB and VapA are normally associated with isolates from pigs and horses, respectively. To contribute to a molecular understanding of Vap function, the crystal structure of a protease-resistant VapB fragment was determined at 1.4 resolution. The structure was solved by SAD phasing employing the anomalous signal of one endogenous S atom and two bound Co ions with low occupancy. VapB is an eight-stranded antiparallel ?-barrel with a single helix. Structural similarity to avidins suggests a potential binding function. Unlike other eight- or ten-stranded ?-barrels found in avidins, bacterial outer membrane proteins, fatty-acid-binding proteins and lysozyme inhibitors, Vaps do not have a next-neighbour arrangement but consist of two Greek-key motifs with strand order 41238567, suggesting an unusual or even unique topology.

  11. Microfluidic devices and methods including porous polymer monoliths

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Microfluidic devices and methods including porous polymer monoliths Citation Details In-Document Search Title: Microfluidic devices and methods including porous polymer monoliths Microfluidic devices and methods including porous polymer monoliths are described. Polymerization techniques may be used to generate porous polymer monoliths having pores defined by a liquid component of a fluid mixture. The fluid mixture may contain iniferters and the resulting porous

  12. DOE Releases Request for Information on Critical Materials, Including Fuel

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cell Platinum Group Metal Catalysts | Department of Energy Releases Request for Information on Critical Materials, Including Fuel Cell Platinum Group Metal Catalysts DOE Releases Request for Information on Critical Materials, Including Fuel Cell Platinum Group Metal Catalysts February 17, 2016 - 3:03pm Addthis The U.S. Department of Energy (DOE) has released a Request for Information (RFI) on critical materials in the energy sector, including fuel cell platinum group metal catalysts. The RFI

  13. Percentage of Total Natural Gas Commercial Deliveries included in Prices

    U.S. Energy Information Administration (EIA) Indexed Site

    City Gate Price Residential Price Percentage of Total Residential Deliveries included in Prices Commercial Price Percentage of Total Commercial Deliveries included in Prices Industrial Price Percentage of Total Industrial Deliveries included in Prices Electric Power Price Period: Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Data Series Area Jan-16 Feb-16 Mar-16 Apr-16 May-16 Jun-16 View History U.S.

  14. Percentage of Total Natural Gas Industrial Deliveries included in Prices

    U.S. Energy Information Administration (EIA) Indexed Site

    Pipeline and Distribution Use Price City Gate Price Residential Price Percentage of Total Residential Deliveries included in Prices Commercial Price Percentage of Total Commercial Deliveries included in Prices Industrial Price Percentage of Total Industrial Deliveries included in Prices Vehicle Fuel Price Electric Power Price Period: Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Data Series Area 2010

  15. Percentage of Total Natural Gas Industrial Deliveries included in Prices

    U.S. Energy Information Administration (EIA) Indexed Site

    City Gate Price Residential Price Percentage of Total Residential Deliveries included in Prices Commercial Price Percentage of Total Commercial Deliveries included in Prices Industrial Price Percentage of Total Industrial Deliveries included in Prices Electric Power Price Period: Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Data Series Area Jan-16 Feb-16 Mar-16 Apr-16 May-16 Jun-16 View History U.S.

  16. Percentage of Total Natural Gas Residential Deliveries included in Prices

    U.S. Energy Information Administration (EIA) Indexed Site

    City Gate Price Residential Price Percentage of Total Residential Deliveries included in Prices Commercial Price Percentage of Total Commercial Deliveries included in Prices Industrial Price Percentage of Total Industrial Deliveries included in Prices Electric Power Price Period: Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Data Series Area Jan-16 Feb-16 Mar-16 Apr-16 May-16 Jun-16 View History U.S.

  17. Comparison of Joint Modeling Approaches Including Eulerian Sliding...

    Office of Scientific and Technical Information (OSTI)

    Eulerian Sliding Interfaces Citation Details In-Document Search Title: Comparison of Joint Modeling Approaches Including Eulerian Sliding Interfaces You are accessing a ...

  18. Microfluidic devices and methods including porous polymer monoliths...

    Office of Scientific and Technical Information (OSTI)

    The fluid mixture may contain iniferters and the resulting porous polymer monolith may include surfaces terminated with iniferter species. Capture molecules may then be grafted to ...

  19. DOE Releases Request for Information on Critical Materials, Including...

    Broader source: Energy.gov (indexed) [DOE]

    including fuel cell platinum group metal catalysts. ... on issues related to the demand, supply, opportunities for ... Announces Second RFI on Rare Earth Metals DOE Announces RFI ...

  20. Introduction to Small-Scale Wind Energy Systems (Including RETScreen...

    Open Energy Info (EERE)

    Case Study) (Webinar) Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Introduction to Small-Scale Wind Energy Systems (Including RETScreen Case Study) (Webinar) Focus...

  1. Natural Gas Deliveries to Commercial Consumers (Including Vehicle...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    California (Million Cubic Feet) Natural Gas Deliveries to Commercial Consumers (Including Vehicle Fuel through 1996) in California (Million Cubic Feet) Year Jan Feb Mar Apr May Jun ...

  2. Numerical simulations for low energy nuclear reactions including...

    Office of Scientific and Technical Information (OSTI)

    Numerical simulations for low energy nuclear reactions including direct channels to validate statistical models Citation Details In-Document Search Title: Numerical simulations for ...

  3. U-182: Microsoft Windows Includes Some Invalid Certificates

    Broader source: Energy.gov [DOE]

    The operating system includes some invalid intermediate certificates. The vulnerability is due to the certificate authorities and not the operating system itself.

  4. Numerical simulations for low energy nuclear reactions including...

    Office of Scientific and Technical Information (OSTI)

    Numerical simulations for low energy nuclear reactions including direct channels to ... Visit OSTI to utilize additional information resources in energy science and technology. A ...

  5. Introduction to Small-Scale Photovoltaic Systems (Including RETScreen...

    Open Energy Info (EERE)

    Photovoltaic Systems (Including RETScreen Case Study) (Webinar) Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Introduction to Small-Scale Photovoltaic Systems...

  6. Measuring and modeling the lifetime of nitrous oxide including...

    Office of Scientific and Technical Information (OSTI)

    Published Article: Measuring and modeling the lifetime of nitrous oxide including its variability: NITROUS OXIDE AND ITS CHANGING LIFETIME Prev Next Title: Measuring and ...

  7. Search for Earth-like planets includes LANL star analysis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    their interiors. Consortium team members at Los Alamos include Joyce Ann Guzik, Paul Bradley, Arthur N. Cox, and Kim Simmons. They will help interpret the stellar oscillation...

  8. Including Retro-Commissioning in Federal Energy Savings Performance Contracts

    Broader source: Energy.gov [DOE]

    Document describes guidance on the importance of (and steps to) including retro-commissioning in federal energy savings performance contracts (ESPCs).

  9. Microfluidic devices and methods including porous polymer monoliths

    DOE Patents [OSTI]

    Hatch, Anson V.; Sommer, Gregory j.; Singh, Anup K.; Wang, Ying-Chih; Abhyankar, Vinay

    2015-12-01

    Microfluidic devices and methods including porous polymer monoliths are described. Polymerization techniques may be used to generate porous polymer monoliths having pores defined by a liquid component of a fluid mixture. The fluid mixture may contain iniferters and the resulting porous polymer monolith may include surfaces terminated with iniferter species. Capture molecules may then be grafted to the monolith pores.

  10. Microfluidic devices and methods including porous polymer monoliths

    DOE Patents [OSTI]

    Hatch, Anson V; Sommer, Gregory J; Singh, Anup K; Wang, Ying-Chih; Abhyankar, Vinay V

    2014-04-22

    Microfluidic devices and methods including porous polymer monoliths are described. Polymerization techniques may be used to generate porous polymer monoliths having pores defined by a liquid component of a fluid mixture. The fluid mixture may contain iniferters and the resulting porous polymer monolith may include surfaces terminated with iniferter species. Capture molecules may then be grafted to the monolith pores.

  11. Solar Energy Education. Reader, Part II. Sun story. [Includes glossary

    SciTech Connect (OSTI)

    Not Available

    1981-05-01

    Magazine articles which focus on the subject of solar energy are presented. The booklet prepared is the second of a four part series of the Solar Energy Reader. Excerpts from the magazines include the history of solar energy, mythology and tales, and selected poetry on the sun. A glossary of energy related terms is included. (BCS)

  12. Articles which include chevron film cooling holes, and related processes

    DOE Patents [OSTI]

    Bunker, Ronald Scott; Lacy, Benjamin Paul

    2014-12-09

    An article is described, including an inner surface which can be exposed to a first fluid; an inlet; and an outer surface spaced from the inner surface, which can be exposed to a hotter second fluid. The article further includes at least one row or other pattern of passage holes. Each passage hole includes an inlet bore extending through the substrate from the inlet at the inner surface to a passage hole-exit proximate to the outer surface, with the inlet bore terminating in a chevron outlet adjacent the hole-exit. The chevron outlet includes a pair of wing troughs having a common surface region between them. The common surface region includes a valley which is adjacent the hole-exit; and a plateau adjacent the valley. The article can be an airfoil. Related methods for preparing the passage holes are also described.

  13. Modular Environment for Graph Research and Analysis with a Persistent

    Energy Science and Technology Software Center (OSTI)

    2009-11-18

    The MEGRAPHS software package provides a front-end to graphs and vectors residing on special-purpose computing resources. It allows these data objects to be instantiated, destroyed, and manipulated. A variety of primitives needed for typical graph analyses are provided. An example program illustrating how MEGRAPHS can be used to implement a PageRank computation is included in the distribution.The MEGRAPHS software package is targeted towards developers of graph algorithms. Programmers using MEGRAPHS would write graph analysis programsmore » in terms of high-level graph and vector operations. These computations are transparently executed on the Cray XMT compute nodes.« less

  14. Turbomachine injection nozzle including a coolant delivery system

    DOE Patents [OSTI]

    Zuo, Baifang (Simpsonville, SC)

    2012-02-14

    An injection nozzle for a turbomachine includes a main body having a first end portion that extends to a second end portion defining an exterior wall having an outer surface. A plurality of fluid delivery tubes extend through the main body. Each of the plurality of fluid delivery tubes includes a first fluid inlet for receiving a first fluid, a second fluid inlet for receiving a second fluid and an outlet. The injection nozzle further includes a coolant delivery system arranged within the main body. The coolant delivery system guides a coolant along at least one of a portion of the exterior wall and around the plurality of fluid delivery tubes.

  15. Example Retro-Commissioning Scope of Work to Include Services...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Services as Part of an ESPC Investment-Grade Audit Example Retro-Commissioning Scope of Work to Include Services as Part of an ESPC Investment-Grade Audit Document offers a ...

  16. Removal of mineral matter including pyrite from coal

    DOE Patents [OSTI]

    Reggel, Leslie; Raymond, Raphael; Blaustein, Bernard D.

    1976-11-23

    Mineral matter, including pyrite, is removed from coal by treatment of the coal with aqueous alkali at a temperature of about 175.degree. to 350.degree. C, followed by acidification with strong acid.

  17. Energy Department Expands Gas Gouging Reporting System to Include...

    Energy Savers [EERE]

    Expands Gas Gouging Reporting System to Include 1-800 Number: 1-800-244-3301 Energy Department Expands Gas ... of reformulated gasoline in storage and is already helping to ...

  18. T-603: Mac OS X Includes Some Invalid Comodo Certificates

    Office of Energy Efficiency and Renewable Energy (EERE)

    The operating system includes some invalid certificates. The vulnerability is due to the invalid certificates and not the operating system itself. Other browsers, applications, and operating systems are affected.

  19. Natural Gas Deliveries to Commercial Consumers (Including Vehicle...

    U.S. Energy Information Administration (EIA) Indexed Site

    Mexico (Million Cubic Feet) Natural Gas Deliveries to Commercial Consumers (Including Vehicle Fuel through 1996) in New Mexico (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul ...

  20. Including Retro-Commissioning in Federal Energy Savings Performance...

    Energy Savers [EERE]

    the cost of the survey. Developing a detailed scope of work and a fixed price for this work is important to eliminate risk to the Agency and the ESCo. Including a detailed scope...

  1. Natural Gas Delivered to Consumers in Ohio (Including Vehicle...

    U.S. Energy Information Administration (EIA) Indexed Site

    Natural Gas Delivered to Consumers in Ohio (Including Vehicle Fuel) (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2001 136,340 110,078 102,451 66,525 ...

  2. Thermodynamic Advantages of Low Temperature Combustion Engines Including

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the Use of Low Heat Rejection Concepts | Department of Energy Advantages of Low Temperature Combustion Engines Including the Use of Low Heat Rejection Concepts Thermodynamic Advantages of Low Temperature Combustion Engines Including the Use of Low Heat Rejection Concepts Thermodynamic cycle simulation was used to evaluate low temperature combustion in systematic and sequential fashion to base engine design. deer10_caton.pdf (462.23 KB) More Documents & Publications Boosted HCCI for High

  3. Hybrid powertrain system including smooth shifting automated transmission

    DOE Patents [OSTI]

    Beaty, Kevin D.; Nellums, Richard A.

    2006-10-24

    A powertrain system is provided that includes a prime mover and a change-gear transmission having an input, at least two gear ratios, and an output. The powertrain system also includes a power shunt configured to route power applied to the transmission by one of the input and the output to the other one of the input and the output. A transmission system and a method for facilitating shifting of a transmission system are also provided.

  4. Prevention of Harassment (Including Sexual Harassment) and Retaliation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Policy Statement | Department of Energy Prevention of Harassment (Including Sexual Harassment) and Retaliation Policy Statement Prevention of Harassment (Including Sexual Harassment) and Retaliation Policy Statement DOE Policy for Preventing Harassment in the Workplace Harassment Policy July 2011.pdf (112.57 KB) More Documents & Publications Policy Statement on Equal Employment Opportunity, Harassment, and Retaliation Equal Employment Opportunity and Diversity Policy Statement VWA-0039 -

  5. Limited Personal Use of Government Office Equipment including Information Technology

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2005-01-07

    The Order establishes requirements and assigns responsibilities for employees' limited personal use of Government resources (office equipment and other resources including information technology) within DOE, including NNSA. The Order is required to provide guidance on appropriate and inappropriate uses of Government resources. This Order was certified 04/23/2009 as accurate and continues to be relevant and appropriate for use by the Department. Certified 4-23-09. No cancellation.

  6. Solar Energy Education. Reader, Part II. Sun story. [Includes glossary]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (Technical Report) | SciTech Connect Reader, Part II. Sun story. [Includes glossary] Citation Details In-Document Search Title: Solar Energy Education. Reader, Part II. Sun story. [Includes glossary] × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy science and

  7. Solar Energy Education. Renewable energy: a background text. [Includes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    glossary] (Technical Report) | SciTech Connect energy: a background text. [Includes glossary] Citation Details In-Document Search Title: Solar Energy Education. Renewable energy: a background text. [Includes glossary] × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy

  8. DOE Revises its NEPA Regulations, Including Categorical Exclusions |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Revises its NEPA Regulations, Including Categorical Exclusions DOE Revises its NEPA Regulations, Including Categorical Exclusions September 30, 2011 - 2:30pm Addthis On September 27, 2011, the Department of Energy (DOE) approved revisions to its National Environmental Policy Act (NEPA) regulations, and on September 28th, submitted the revisions to the Federal Register. The final regulations, which become effective 30 days after publication in the Federal Register, are

  9. Summary of the Persistent Current Effect Measurements in Nb3Sn and NbTi Accelerator Magnets at Fermilab

    SciTech Connect (OSTI)

    Velev, G.V.; Chlachidze, G.; DiMarco, J.; Stoynev, S.E.

    2015-10-01

    In the past 10 years, Fermilab has been executing an intensive R&D program on accelerator magnets based on Nb3Sn superconductor technology. This R&D effort includes dipole and quadrupole models for different programs, such as LARP and 11 T dipoles for the LHC high-luminosity upgrade. Before the Nb3Sn R&D program, Fermilab was involved in the production of the low-beta quadrupole magnets for LHC based on the NbTi superconductor. Additionally, during the 2003-2005 campaign to optimize the operation of the Tevatron, a large number of Tevatron magnets were re-measured. As a result of this field analysis, a systematic study of the persistent current decay and snapback effect in these magnets was performed. This paper summarizes the result of this study and presents a comparison between Nb3Sn and NbTi dipoles and quadrupoles.

  10. Modeling Exposure to Persistent Chemicals in Hazard and Risk Assessment

    SciTech Connect (OSTI)

    Cowan-Ellsberry, Christina E.; McLachlan, Michael S.; Arnot, Jon A.; MacLeod, Matthew; McKone, Thomas E.; Wania, Frank

    2008-11-01

    Fate and exposure modeling has not thus far been explicitly used in the risk profile documents prepared to evaluate significant adverse effect of candidate chemicals for either the Stockholm Convention or the Convention on Long-Range Transboundary Air Pollution. However, we believe models have considerable potential to improve the risk profiles. Fate and exposure models are already used routinely in other similar regulatory applications to inform decisions, and they have been instrumental in building our current understanding of the fate of POP and PBT chemicals in the environment. The goal of this paper is to motivate the use of fate and exposure models in preparing risk profiles in the POP assessment procedure by providing strategies for incorporating and using models. The ways that fate and exposure models can be used to improve and inform the development of risk profiles include: (1) Benchmarking the ratio of exposure and emissions of candidate chemicals to the same ratio for known POPs, thereby opening the possibility of combining this ratio with the relative emissions and relative toxicity to arrive at a measure of relative risk. (2) Directly estimating the exposure of the environment, biota and humans to provide information to complement measurements, or where measurements are not available or are limited. (3) To identify the key processes and chemical and/or environmental parameters that determine the exposure; thereby allowing the effective prioritization of research or measurements to improve the risk profile. (4) Predicting future time trends including how quickly exposure levels in remote areas would respond to reductions in emissions. Currently there is no standardized consensus model for use in the risk profile context. Therefore, to choose the appropriate model the risk profile developer must evaluate how appropriate an existing model is for a specific setting and whether the assumptions and input data are relevant in the context of the application

  11. Trench logs from a strand of the Rock Valley Fault System, Nevada Test Site, Nye County, Nevada

    SciTech Connect (OSTI)

    Yount, J.C.; Shroba, R.R.; McMasters, C.R.; Huckins, H.E.; Rodriguez, E.A.

    1987-12-31

    The Rock Valley fault system trends northeasterly through the southeast corner of the Nevada Test Site. The system records left-lateral offset of Paleozoic and Tertiary rocks, although total offset amounts to only a few kilometers. Distinct scarps in alluvial deposits of Quaternary age and a concentration of seismicity, particularly at its north end, suggest that the Rock Valley fault system may be active. Two trenches were excavated by backhoe in 1978 across a 0.5-m-high scarp produced by a strand of the Rock Valley fault system. A detailed logging of the two Rock Valley fault trenches was undertaken during the spring of 1984. This report presents: (1) logs of both walls of the two trenches, (2) a general description of the lithologic units and the soils formed in these units that are exposed in and near the fault trenches, (3) observations of the clast fabric of unfaulted and faulted deposits exposed in the trench walls, and (4) a map of the surficial deposits in the vicinity of the trenches.

  12. A model for heterogeneous materials including phase transformations

    SciTech Connect (OSTI)

    Addessio, F.L.; Clements, B.E.; Williams, T.O.

    2005-04-15

    A model is developed for particulate composites, which includes phase transformations in one or all of the constituents. The model is an extension of the method of cells formalism. Representative simulations for a single-phase, brittle particulate (SiC) embedded in a ductile material (Ti), which undergoes a solid-solid phase transformation, are provided. Also, simulations for a tungsten heavy alloy (WHA) are included. In the WHA analyses a particulate composite, composed of tungsten particles embedded in a tungsten-iron-nickel alloy matrix, is modeled. A solid-liquid phase transformation of the matrix material is included in the WHA numerical calculations. The example problems also demonstrate two approaches for generating free energies for the material constituents. Simulations for volumetric compression, uniaxial strain, biaxial strain, and pure shear are used to demonstrate the versatility of the model.

  13. Methods of producing adsorption media including a metal oxide

    DOE Patents [OSTI]

    Mann, Nicholas R; Tranter, Troy J

    2014-03-04

    Methods of producing a metal oxide are disclosed. The method comprises dissolving a metal salt in a reaction solvent to form a metal salt/reaction solvent solution. The metal salt is converted to a metal oxide and a caustic solution is added to the metal oxide/reaction solvent solution to adjust the pH of the metal oxide/reaction solvent solution to less than approximately 7.0. The metal oxide is precipitated and recovered. A method of producing adsorption media including the metal oxide is also disclosed, as is a precursor of an active component including particles of a metal oxide.

  14. Metal vapor laser including hot electrodes and integral wick

    DOE Patents [OSTI]

    Ault, E.R.; Alger, T.W.

    1995-03-07

    A metal vapor laser, specifically one utilizing copper vapor, is disclosed herein. This laser utilizes a plasma tube assembly including a thermally insulated plasma tube containing a specific metal, e.g., copper, and a buffer gas therein. The laser also utilizes means including hot electrodes located at opposite ends of the plasma tube for electrically exciting the metal vapor and heating its interior to a sufficiently high temperature to cause the metal contained therein to vaporize and for subjecting the vapor to an electrical discharge excitation in order to lase. The laser also utilizes external wicking arrangements, that is, wicking arrangements located outside the plasma tube. 5 figs.

  15. Metal vapor laser including hot electrodes and integral wick

    DOE Patents [OSTI]

    Ault, Earl R.; Alger, Terry W.

    1995-01-01

    A metal vapor laser, specifically one utilizing copper vapor, is disclosed herein. This laser utilizes a plasma tube assembly including a thermally insulated plasma tube containing a specific metal, e.g., copper, and a buffer gas therein. The laser also utilizes means including hot electrodes located at opposite ends of the plasma tube for electrically exciting the metal vapor and heating its interior to a sufficiently high temperature to cause the metal contained therein to vaporize and for subjecting the vapor to an electrical discharge excitation in order to lase. The laser also utilizes external wicking arrangements, that is, wicking arrangements located outside the plasma tube.

  16. Solar Energy Education. Renewable energy: a background text. [Includes glossary

    SciTech Connect (OSTI)

    Not Available

    1985-01-01

    Some of the most common forms of renewable energy are presented in this textbook for students. The topics include solar energy, wind power hydroelectric power, biomass ocean thermal energy, and tidal and geothermal energy. The main emphasis of the text is on the sun and the solar energy that it yields. Discussions on the sun's composition and the relationship between the earth, sun and atmosphere are provided. Insolation, active and passive solar systems, and solar collectors are the subtopics included under solar energy. (BCS)

  17. Tunable cavity resonator including a plurality of MEMS beams

    DOE Patents [OSTI]

    Peroulis, Dimitrios; Fruehling, Adam; Small, Joshua Azariah; Liu, Xiaoguang; Irshad, Wasim; Arif, Muhammad Shoaib

    2015-10-20

    A tunable cavity resonator includes a substrate, a cap structure, and a tuning assembly. The cap structure extends from the substrate, and at least one of the substrate and the cap structure defines a resonator cavity. The tuning assembly is positioned at least partially within the resonator cavity. The tuning assembly includes a plurality of fixed-fixed MEMS beams configured for controllable movement relative to the substrate between an activated position and a deactivated position in order to tune a resonant frequency of the tunable cavity resonator.

  18. DOE Considers Natural Gas Utility Service Options: Proposal Includes

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    30-mile Natural Gas Pipeline from Pasco to Hanford | Department of Energy Considers Natural Gas Utility Service Options: Proposal Includes 30-mile Natural Gas Pipeline from Pasco to Hanford DOE Considers Natural Gas Utility Service Options: Proposal Includes 30-mile Natural Gas Pipeline from Pasco to Hanford January 23, 2012 - 12:00pm Addthis Media Contacts Cameron Hardy, DOE , (509) 376-5365, Cameron.Hardy@rl.doe.gov RICHLAND, WASH. - The U.S. Department of Energy (DOE) is considering

  19. Thin film solar cell including a spatially modulated intrinsic layer

    SciTech Connect (OSTI)

    Guha, Subhendu; Yang, Chi-Chung; Ovshinsky, Stanford R.

    1989-03-28

    One or more thin film solar cells in which the intrinsic layer of substantially amorphous semiconductor alloy material thereof includes at least a first band gap portion and a narrower band gap portion. The band gap of the intrinsic layer is spatially graded through a portion of the bulk thickness, said graded portion including a region removed from the intrinsic layer-dopant layer interfaces. The band gap of the intrinsic layer is always less than the band gap of the doped layers. The gradation of the intrinsic layer is effected such that the open circuit voltage and/or the fill factor of the one or plural solar cell structure is enhanced.

  20. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, N.V.; Broekaert, W.F.; Namhai Chua; Kush, A.

    1993-02-16

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids.

  1. Information regarding previous INCITE awards including selected highlights

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | U.S. DOE Office of Science (SC) Information regarding previous INCITE awards including selected highlights Advanced Scientific Computing Research (ASCR) ASCR Home About Research Facilities User Facilities Accessing ASCR Facilities Innovative & Novel Computational Impact on Theory & Experiement (INCITE) ASCR Leadership Computing Challenge (ALCC) Industrial Users Computational Science Graduate Fellowship (CSGF) Research & Evaluation Prototypes (REP) Science Highlights Benefits of

  2. Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases

    SciTech Connect (OSTI)

    Shimobayashi, Shunsuke F.; Iwaki, Takafumi; Mori, Toshiaki; Yoshikawa, Kenichi

    2013-05-07

    By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.

  3. Chemical repair of base lesions, AP-sites, and strand breaks on plasmid DNA in dilute aqueous solution by ascorbic acid

    SciTech Connect (OSTI)

    Hata, Kuniki; Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1195 ; Urushibara, Ayumi; Yamashita, Shinichi; Shikazono, Naoya; Yokoya, Akinari; Katsumura, Yosuke; Nuclear Professional School, School of Engineering, The University of Tokyo, 2-22 Shirakatashirane, Tokai-mura, Naka-gun, Ibaraki 319-1188

    2013-05-03

    Highlights: We report a novel mechanism of radiation protection of DNA by chemical activity of ascorbic acid. The chemical repair of DNA damage was revealed using biochemical assay and chemical kinetics analysis. We found that ascorbic acid significantly repairs precursors of nucleobase lesions and abasic sites. However, ascorbic acid seldom repairs precursors of DNA-strand breaks. -- Abstract: We quantified the damage yields produced in plasmid DNA by ?-irradiation in the presence of low concentrations (10100 ?M) of ascorbic acid, which is a major antioxidant in living systems, to clarify whether it chemically repairs radiation damage in DNA. The yield of DNA single strand breaks induced by irradiation was analyzed with agarose gel electrophoresis as conformational changes in closed circular plasmids. Base lesions and abasic sites were also observed as additional conformational changes by treating irradiated samples with glycosylase proteins. By comparing the suppression efficiencies to the induction of each DNA lesion, in addition to scavenging of the OH radicals derived from water radiolysis, it was found that ascorbic acid promotes the chemical repair of precursors of AP-sites and base lesions more effectively than those of single strand breaks. We estimated the efficiency of the chemical repair of each lesion using a kinetic model. Approximately 5060% of base lesions and AP-sites were repaired by 10 ?M ascorbic acid, although strand breaks were largely unrepaired by ascorbic acid at low concentrations. The methods in this study will provide a route to understanding the mechanistic aspects of antioxidant activity in living systems.

  4. [Article 1 of 7: Motivates and Includes the Consumer]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 of 7: Research on the Characteristics of a Modern Grid by the NETL Modern Grid Strategy Team Accommodates All Generation and Storage Options Last month we presented the first Principal Characteristic of a Modern Grid, "Motivates and Includes the Consumer". This month we present a second characteristic, "Accommodates All Generation and Storage Options". This characteristic will fundamentally transition today's grid from a centralized model for generation to one that also has

  5. [Article 1 of 7: Motivates and Includes the Consumer]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Series on the Seven Principal Characteristics of the Modern Grid [Article 1 of 7: Motivates and Includes the Consumer] In October 2007, Ken Silverstein (Energy Central) wrote an editorial, "Empowering Consumers" that hit a strong, kindred chord with the DOE/National Energy Technology Laboratory (NETL) Modern Grid Strategy team. Through subsequent discussions with Ken and Bill Opalka, Editor- In-Chief, Topics Centers, we decided it would be informative to the industry if the Modern Grid

  6. Evaporative cooler including one or more rotating cooler louvers

    DOE Patents [OSTI]

    Gerlach, David W

    2015-02-03

    An evaporative cooler may include an evaporative cooler housing with a duct extending therethrough, a plurality of cooler louvers with respective porous evaporative cooler pads, and a working fluid source conduit. The cooler louvers are arranged within the duct and rotatably connected to the cooler housing along respective louver axes. The source conduit provides an evaporative cooler working fluid to the cooler pads during at least one mode of operation.

  7. Conversion of geothermal waste to commercial products including silica

    DOE Patents [OSTI]

    Premuzic, Eugene T.; Lin, Mow S.

    2003-01-01

    A process for the treatment of geothermal residue includes contacting the pigmented amorphous silica-containing component with a depigmenting reagent one or more times to depigment the silica and produce a mixture containing depigmented amorphous silica and depigmenting reagent containing pigment material; separating the depigmented amorphous silica and from the depigmenting reagent to yield depigmented amorphous silica. Before or after the depigmenting contacting, the geothermal residue or depigmented silica can be treated with a metal solubilizing agent to produce another mixture containing pigmented or unpigmented amorphous silica-containing component and a solubilized metal-containing component; separating these components from each other to produce an amorphous silica product substantially devoid of metals and at least partially devoid of pigment. The amorphous silica product can be neutralized and thereafter dried at a temperature from about 25.degree. C. to 300.degree. C. The morphology of the silica product can be varied through the process conditions including sequence contacting steps, pH of depigmenting reagent, neutralization and drying conditions to tailor the amorphous silica for commercial use in products including filler for paint, paper, rubber and polymers, and chromatographic material.

  8. Electrolytes including fluorinated solvents for use in electrochemical cells

    DOE Patents [OSTI]

    Tikhonov, Konstantin; Yip, Ka Ki; Lin, Tzu-Yuan

    2015-07-07

    Provided are electrochemical cells and electrolytes used to build such cells. The electrolytes include ion-supplying salts and fluorinated solvents capable of maintaining single phase solutions with the salts at between about -30.degree. C. to about 80.degree. C. The fluorinated solvents, such as fluorinated carbonates, fluorinated esters, and fluorinated esters, are less flammable than their non-fluorinated counterparts and increase safety characteristics of cells containing these solvents. The amount of fluorinated solvents in electrolytes may be between about 30% and 80% by weight not accounting weight of the salts. Fluorinated salts, such as fluoroalkyl-substituted LiPF.sub.6, fluoroalkyl-substituted LiBF.sub.4 salts, linear and cyclic imide salts as well as methide salts including fluorinated alkyl groups, may be used due to their solubility in the fluorinated solvents. In some embodiments, the electrolyte may also include a flame retardant, such as a phosphazene or, more specifically, a cyclic phosphazene and/or one or more ionic liquids.

  9. Multi-processor including data flow accelerator module

    DOE Patents [OSTI]

    Davidson, George S.; Pierce, Paul E.

    1990-01-01

    An accelerator module for a data flow computer includes an intelligent memory. The module is added to a multiprocessor arrangement and uses a shared tagged memory architecture in the data flow computer. The intelligent memory module assigns locations for holding data values in correspondence with arcs leading to a node in a data dependency graph. Each primitive computation is associated with a corresponding memory cell, including a number of slots for operands needed to execute a primitive computation, a primitive identifying pointer, and linking slots for distributing the result of the cell computation to other cells requiring that result as an operand. Circuitry is provided for utilizing tag bits to determine automatically when all operands required by a processor are available and for scheduling the primitive for execution in a queue. Each memory cell of the module may be associated with any of the primitives, and the particular primitive to be executed by the processor associated with the cell is identified by providing an index, such as the cell number for the primitive, to the primitive lookup table of starting addresses. The module thus serves to perform functions previously performed by a number of sections of data flow architectures and coexists with conventional shared memory therein. A multiprocessing system including the module operates in a hybrid mode, wherein the same processing modules are used to perform some processing in a sequential mode, under immediate control of an operating system, while performing other processing in a data flow mode.

  10. Man-made marine debris and sea turtle strandings on beaches of the upper Texas and southwestern Louisiana coasts, June 1987 through September 1989. Technical memo

    SciTech Connect (OSTI)

    Duronslet, M.J.; Revera, D.B.; Stanley, K.M.

    1991-02-01

    The upper Texas and southwestern Louisiana coastlines were divided into six sampling zones to survey the amounts, types and rates of accumulation of man-made marine debris, the number of sea turtle strandings, the incidence of sea turtle entanglements in marine debris and the incidence of ingestion of such debris by sea turtles. From June 1987 through September 1989, 473 sample plots were examined for marine debris. Significant differences were detected in mean number of debris items per 100 sq m of beach sampled by year, zone and month. Significant differences in mean weight of debris items per 100 sq m of beach sampled were detected by month and zone. Both number and weights (per 100 sq m) of debris were lowest in the winter months. Number per 100 sq m was greatest in August while weight per 100 sq m peaked in May. Tar balls and plastic items were the most frequently encountered marine debris items. Wooden items had the highest average weights while tar balls and polystyrene foam were the lightest items collected. A total of 171 sea turtles stranded on the surveyed beaches during the study. Of 26 gastrointestinal tracts examined, 16 had ingested some form of man-made debris. Six turtles were entangled in man-made debris and 9 were live stranded.

  11. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, Natasha V.; Broekaert, Willem F.; Chua, Nam-Hai; Kush, Anil

    1993-02-16

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a pu GOVERNMENT RIGHTS This application was funded under Department of Energy Contract DE-AC02-76ER01338. The U.S. Government has certain rights under this application and any patent issuing thereon.

  12. NREL: Technology Deployment - Cities-LEAP Energy Profile Tool Includes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Data on More than 23,400 U.S. Cities Cities-LEAP Energy Profile Tool Includes Energy Data on More than 23,400 U.S. Cities News NREL Report Examines Energy Use in Cities and Proposes Next Steps for Energy Innovation Publications Citi-Level Energy Decision Making: Data Use in Energy Planning, Implementation, and Evaluation in U.S. Cities Sponsors DOE's Energy Office of Energy Efficiency and Renewable Energy Policy and Analysis Office Related Stories Hawaii's First Net-Zero Energy

  13. What To Include In The Whistleblower Complaint? | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration | (NNSA) To Include In The Whistleblower Complaint? Your complaint does not need to be in any specific form but must be signed by you and contain the following: A statement specifically describing 1. The alleged retaliation taken against you and 2. The disclosure, participation, or refusal that you believe gave rise to the retaliation; A statement that you are not currently pursuing a remedy under State or other applicable law, as described in Sec. 708.15 of this subpart; A

  14. Composite material including nanocrystals and methods of making

    DOE Patents [OSTI]

    Bawendi, Moungi G.; Sundar, Vikram C.

    2008-02-05

    Temperature-sensing compositions can include an inorganic material, such as a semiconductor nanocrystal. The nanocrystal can be a dependable and accurate indicator of temperature. The intensity of emission of the nanocrystal varies with temperature and can be highly sensitive to surface temperature. The nanocrystals can be processed with a binder to form a matrix, which can be varied by altering the chemical nature of the surface of the nanocrystal. A nanocrystal with a compatibilizing outer layer can be incorporated into a coating formulation and retain its temperature sensitive emissive properties

  15. Composite material including nanocrystals and methods of making

    DOE Patents [OSTI]

    Bawendi, Moungi G.; Sundar, Vikram C.

    2010-04-06

    Temperature-sensing compositions can include an inorganic material, such as a semiconductor nanocrystal. The nanocrystal can be a dependable and accurate indicator of temperature. The intensity of emission of the nanocrystal varies with temperature and can be highly sensitive to surface temperature. The nanocrystals can be processed with a binder to form a matrix, which can be varied by altering the chemical nature of the surface of the nanocrystal. A nanocrystal with a compatibilizing outer layer can be incorporated into a coating formulation and retain its temperature sensitive emissive properties.

  16. A coke oven model including thermal decomposition kinetics of tar

    SciTech Connect (OSTI)

    Munekane, Fuminori; Yamaguchi, Yukio; Tanioka, Seiichi

    1997-12-31

    A new one-dimensional coke oven model has been developed for simulating the amount and the characteristics of by-products such as tar and gas as well as coke. This model consists of both heat transfer and chemical kinetics including thermal decomposition of coal and tar. The chemical kinetics constants are obtained by estimation based on the results of experiments conducted to investigate the thermal decomposition of both coal and tar. The calculation results using the new model are in good agreement with experimental ones.

  17. Composite armor, armor system and vehicle including armor system

    DOE Patents [OSTI]

    Chu, Henry S.; Jones, Warren F.; Lacy, Jeffrey M.; Thinnes, Gary L.

    2013-01-01

    Composite armor panels are disclosed. Each panel comprises a plurality of functional layers comprising at least an outermost layer, an intermediate layer and a base layer. An armor system incorporating armor panels is also disclosed. Armor panels are mounted on carriages movably secured to adjacent rails of a rail system. Each panel may be moved on its associated rail and into partially overlapping relationship with another panel on an adjacent rail for protection against incoming ordnance from various directions. The rail system may be configured as at least a part of a ring, and be disposed about a hatch on a vehicle. Vehicles including an armor system are also disclosed.

  18. Community Assessment Tool for Public Health Emergencies Including Pandemic Influenza

    SciTech Connect (OSTI)

    ORAU's Oak Ridge Institute for Science Education

    2011-04-14

    The Community Assessment Tool (CAT) for Public Health Emergencies Including Pandemic Influenza (hereafter referred to as the CAT) was developed as a result of feedback received from several communities. These communities participated in workshops focused on influenza pandemic planning and response. The 2008 through 2011 workshops were sponsored by the Centers for Disease Control and Prevention (CDC). Feedback during those workshops indicated the need for a tool that a community can use to assess its readiness for a disaster - readiness from a total healthcare perspective, not just hospitals, but the whole healthcare system. The CAT intends to do just that - help strengthen existing preparedness plans by allowing the healthcare system and other agencies to work together during an influenza pandemic. It helps reveal each core agency partners (sectors) capabilities and resources, and highlights cases of the same vendors being used for resource supplies (e.g., personal protective equipment [PPE] and oxygen) by the partners (e.g., public health departments, clinics, or hospitals). The CAT also addresses gaps in the community's capabilities or potential shortages in resources. This tool has been reviewed by a variety of key subject matter experts from federal, state, and local agencies and organizations. It also has been piloted with various communities that consist of different population sizes, to include large urban to small rural communities.

  19. Copper laser modulator driving assembly including a magnetic compression laser

    DOE Patents [OSTI]

    Cook, Edward G.; Birx, Daniel L.; Ball, Don G.

    1994-01-01

    A laser modulator (10) having a low voltage assembly (12) with a plurality of low voltage modules (14) with first stage magnetic compression circuits (20) and magnetic assist inductors (28) with a common core (91), such that timing of the first stage magnetic switches (30b) is thereby synchronized. A bipolar second stage of magnetic compression (42) is coupled to the low voltage modules (14) through a bipolar pulse transformer (36) and a third stage of magnetic compression (44) is directly coupled to the second stage of magnetic compression (42). The low voltage assembly (12) includes pressurized boxes (117) for improving voltage standoff between the primary winding assemblies (34) and secondary winding (40) contained therein.

  20. Nijmegen soft-core potential including two-meson exchange

    SciTech Connect (OSTI)

    Stoks, V.G.J.; Rijken, T.A.

    1995-05-10

    We report on the progress of the construction of the extended soft-core (ESC) Nijmegen potential. Next to the standard one-boson-exchange parts, the model includes the pion-meson-exchange potentials due to the parallel and crossed-box diagrams, as well as the one-pair and two-pair diagrams, vertices for which can be identified with similar interactions appearing in chiral-symmetric Lagrangians. Although the ESC potential is still under construction, it already gives an excellent description of all {ital NN} scattering data below 350 MeV with {chi}{sup 2}/datum=1.3. {copyright} {ital 1995} {ital American} {ital Institute} {ital of} {ital Physics}.

  1. Fuel cell repeater unit including frame and separator plate

    DOE Patents [OSTI]

    Yamanis, Jean; Hawkes, Justin R; Chiapetta, Jr., Louis; Bird, Connie E; Sun, Ellen Y; Croteau, Paul F

    2013-11-05

    An example fuel cell repeater includes a separator plate and a frame establishing at least a portion of a flow path that is operative to communicate fuel to or from at least one fuel cell held by the frame relative to the separator plate. The flow path has a perimeter and any fuel within the perimeter flow across the at least one fuel cell in a first direction. The separator plate, the frame, or both establish at least one conduit positioned outside the flow path perimeter. The conduit is outside of the flow path perimeter and is configured to direct flow in a second, different direction. The conduit is fluidly coupled with the flow path.

  2. Hydraulic engine valve actuation system including independent feedback control

    DOE Patents [OSTI]

    Marriott, Craig D

    2013-06-04

    A hydraulic valve actuation assembly may include a housing, a piston, a supply control valve, a closing control valve, and an opening control valve. The housing may define a first fluid chamber, a second fluid chamber, and a third fluid chamber. The piston may be axially secured to an engine valve and located within the first, second and third fluid chambers. The supply control valve may control a hydraulic fluid supply to the piston. The closing control valve may be located between the supply control valve and the second fluid chamber and may control fluid flow from the second fluid chamber to the supply control valve. The opening control valve may be located between the supply control valve and the second fluid chamber and may control fluid flow from the supply control valve to the second fluid chamber.

  3. Recent progress and advances in iterative software (including parallel aspects)

    SciTech Connect (OSTI)

    Carey, G.; Young, D.M.; Kincaid, D.

    1994-12-31

    The purpose of the workshop is to provide a forum for discussion of the current state of iterative software packages. Of particular interest is software for large scale engineering and scientific applications, especially for distributed parallel systems. However, the authors will also review the state of software development for conventional architectures. This workshop will complement the other proposed workshops on iterative BLAS kernels and applications. The format for the workshop is as follows: To provide some structure, there will be brief presentations, each of less than five minutes duration and dealing with specific facets of the subject. These will be designed to focus the discussion and to stimulate an exchange with the participants. Issues to be covered include: The evolution of iterative packages, current state of the art, the parallel computing challenge, applications viewpoint, standards, and future directions and open problems.

  4. Actuator assembly including a single axis of rotation locking member

    DOE Patents [OSTI]

    Quitmeyer, James N.; Benson, Dwayne M.; Geck, Kellan P.

    2009-12-08

    An actuator assembly including an actuator housing assembly and a single axis of rotation locking member fixedly attached to a portion of the actuator housing assembly and an external mounting structure. The single axis of rotation locking member restricting rotational movement of the actuator housing assembly about at least one axis. The single axis of rotation locking member is coupled at a first end to the actuator housing assembly about a Y axis and at a 90.degree. angle to an X and Z axis providing rotation of the actuator housing assembly about the Y axis. The single axis of rotation locking member is coupled at a second end to a mounting structure, and more particularly a mounting pin, about an X axis and at a 90.degree. angle to a Y and Z axis providing rotation of the actuator housing assembly about the X axis. The actuator assembly is thereby restricted from rotation about the Z axis.

  5. Dye laser amplifier including a specifically designed diffuser assembly

    DOE Patents [OSTI]

    Davin, James; Johnston, James P.

    1992-01-01

    A large (high flow rate) dye laser amplifier in which a continuous replened supply of dye is excited by a first light beam, specifically a copper vapor laser beam, in order to amplify the intensity of a second different light beam, specifically a dye beam, passing through the dye is disclosed herein. This amplifier includes a dye cell defining a dye chamber through which a continuous stream of dye is caused to pass at a relatively high flow rate and a specifically designed diffuser assembly for slowing down the flow of dye while, at the same time, assuring that as the dye stream flows through the diffuser assembly it does so in a stable manner.

  6. Electra-optical device including a nitrogen containing electrolyte

    DOE Patents [OSTI]

    Bates, J.B.; Dudney, N.J.; Gruzalski, G.R.; Luck, C.F.

    1995-10-03

    Described is a thin-film battery, especially a thin-film microbattery, and a method for making same having application as a backup or primary integrated power source for electronic devices. The battery includes a novel electrolyte which is electrochemically stable and does not react with the lithium anode and a novel vanadium oxide cathode. Configured as a microbattery, the battery can be fabricated directly onto a semiconductor chip, onto the semiconductor die or onto any portion of the chip carrier. The battery can be fabricated to any specified size or shape to meet the requirements of a particular application. The battery is fabricated of solid state materials and is capable of operation between {minus}15 C and 150 C.

  7. Electra-optical device including a nitrogen containing electrolyte

    DOE Patents [OSTI]

    Bates, John B.; Dudney, Nancy J.; Gruzalski, Greg R.; Luck, Christopher F.

    1995-01-01

    Described is a thin-film battery, especially a thin-film microbattery, and a method for making same having application as a backup or primary integrated power source for electronic devices. The battery includes a novel electrolyte which is electrochemically stable and does not react with the lithium anode and a novel vanadium oxide cathode Configured as a microbattery, the battery can be fabricated directly onto a semiconductor chip, onto the semiconductor die or onto any portion of the chip carrier. The battery can be fabricated to any specified size or shape to meet the requirements of a particular application. The battery is fabricated of solid state materials and is capable of operation between -15.degree. C. and 150.degree. C.

  8. Pulse transmission transmitter including a higher order time derivate filter

    DOE Patents [OSTI]

    Dress, Jr., William B.; Smith, Stephen F.

    2003-09-23

    Systems and methods for pulse-transmission low-power communication modes are disclosed. A pulse transmission transmitter includes: a clock; a pseudorandom polynomial generator coupled to the clock, the pseudorandom polynomial generator having a polynomial load input; an exclusive-OR gate coupled to the pseudorandom polynomial generator, the exclusive-OR gate having a serial data input; a programmable delay circuit coupled to both the clock and the exclusive-OR gate; a pulse generator coupled to the programmable delay circuit; and a higher order time derivative filter coupled to the pulse generator. The systems and methods significantly reduce lower-frequency emissions from pulse transmission spread-spectrum communication modes, which reduces potentially harmful interference to existing radio frequency services and users and also simultaneously permit transmission of multiple data bits by utilizing specific pulse shapes.

  9. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, N.V.; Broekaert, W.F.; Chua, N.H.; Kush, A.

    1999-05-04

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74--79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli. 12 figs.

  10. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, Natasha V.; Broekaert, Willem F.; Chua, Nam-Hai; Kush, Anil

    1999-05-04

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  11. CDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, Natasha V.; Broekaert, Willem F.; Chua, Nam-Hai; Kush, Anil

    1995-03-21

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

  12. Extractant composition including crown ether and calixarene extractants

    DOE Patents [OSTI]

    Meikrantz, David H.; Todd, Terry A.; Riddle, Catherine L.; Law, Jack D.; Peterman, Dean R.; Mincher, Bruce J.; McGrath, Christopher A.; Baker, John D.

    2009-04-28

    An extractant composition comprising a mixed extractant solvent consisting of calix[4] arene-bis-(tert-octylbenzo)-crown-6 ("BOBCalixC6"), 4',4',(5')-di-(t-butyldicyclo-hexano)-18-crown-6 ("DtBu18C6"), and at least one modifier dissolved in a diluent. The DtBu18C6 may be present at from approximately 0.01M to approximately 0.4M, such as at from approximately 0.086 M to approximately 0.108 M. The modifier may be 1-(2,2,3,3-tetrafluoropropoxy)-3-(4-sec-butylphenoxy)-2-propanol ("Cs-7SB") and may be present at from approximately 0.01M to approximately 0.8M. In one embodiment, the mixed extractant solvent includes approximately 0.15M DtBu18C6, approximately 0.007M BOBCalixC6, and approximately 0.75M Cs-7SB modifier dissolved in an isoparaffinic hydrocarbon diluent. The extractant composition further comprises an aqueous phase. The mixed extractant solvent may be used to remove cesium and strontium from the aqueous phase.

  13. C -parameter distribution at N 3 LL ' including power corrections

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hoang, André H.; Kolodrubetz, Daniel W.; Mateu, Vicent; Stewart, Iain W.

    2015-05-15

    We compute the e⁺e⁻ C-parameter distribution using the soft-collinear effective theory with a resummation to next-to-next-to-next-to-leading-log prime accuracy of the most singular partonic terms. This includes the known fixed-order QCD results up to O(α3s), a numerical determination of the two-loop nonlogarithmic term of the soft function, and all logarithmic terms in the jet and soft functions up to three loops. Our result holds for C in the peak, tail, and far tail regions. Additionally, we treat hadronization effects using a field theoretic nonperturbative soft function, with moments Ωn. To eliminate an O(ΛQCD) renormalon ambiguity in the soft function, we switchmore » from the MS¯ to a short distance “Rgap” scheme to define the leading power correction parameter Ω1. We show how to simultaneously account for running effects in Ω1 due to renormalon subtractions and hadron-mass effects, enabling power correction universality between C-parameter and thrust to be tested in our setup. We discuss in detail the impact of resummation and renormalon subtractions on the convergence. In the relevant fit region for αs(mZ) and Ω1, the perturbative uncertainty in our cross section is ≅ 2.5% at Q=mZ.« less

  14. Interim performance criteria for photovoltaic energy systems. [Glossary included

    SciTech Connect (OSTI)

    DeBlasio, R.; Forman, S.; Hogan, S.; Nuss, G.; Post, H.; Ross, R.; Schafft, H.

    1980-12-01

    This document is a response to the Photovoltaic Research, Development, and Demonstration Act of 1978 (P.L. 95-590) which required the generation of performance criteria for photovoltaic energy systems. Since the document is evolutionary and will be updated, the term interim is used. More than 50 experts in the photovoltaic field have contributed in the writing and review of the 179 performance criteria listed in this document. The performance criteria address characteristics of present-day photovoltaic systems that are of interest to manufacturers, government agencies, purchasers, and all others interested in various aspects of photovoltaic system performance and safety. The performance criteria apply to the system as a whole and to its possible subsystems: array, power conditioning, monitor and control, storage, cabling, and power distribution. They are further categorized according to the following performance attributes: electrical, thermal, mechanical/structural, safety, durability/reliability, installation/operation/maintenance, and building/site. Each criterion contains a statement of expected performance (nonprescriptive), a method of evaluation, and a commentary with further information or justification. Over 50 references for background information are also given. A glossary with definitions relevant to photovoltaic systems and a section on test methods are presented in the appendices. Twenty test methods are included to measure performance characteristics of the subsystem elements. These test methods and other parts of the document will be expanded or revised as future experience and needs dictate.

  15. cDNA encoding a polypeptide including a hevein sequence

    DOE Patents [OSTI]

    Raikhel, N.V.; Broekaert, W.F.; Chua, N.H.; Kush, A.

    1995-03-21

    A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1,018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74--79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli. 11 figures.

  16. Transient oxidative stress and inflammation after intraperitoneal administration of multiwalled carbon nanotubes functionalized with single strand DNA in rats

    SciTech Connect (OSTI)

    Clichici, Simona; Biris, Alexandru Radu; Tabaran, Flaviu; Filip, Adriana

    2012-03-15

    Multi-walled carbon nanotubes (MWCNTs) are widely used for nanotechnology. Their impact on living organisms is, however, not entirely clarified. Oxidative stress and inflammation seem to be the key mechanisms involved in MWCNTs' cytotoxicity. Until present, pulmonary and skin models were the main tested experimental designs to assess carbon nanotubes' toxicity. The systemic administration of MWCNTs is essential, with respect for future medical applications. Our research is performed on Wistar rats and is focused on the dynamics of oxidative stress parameters in blood and liver and pro-inflammatory cytokines in liver, after single dose (270 mg l{sup −1}) ip administration of MWCNTs (exterior diameter 15–25 nm, interior diameter 10–15 nm, surface 88 m{sup 2} g{sup −1}) functionalized with single strand DNA (ss-DNA). The presence of MWCNTs in blood was assessed by Raman spectroscopy, while in liver histological examination and confocal microscopy were used. It was found that ss-DNA-MWCNTs induce oxidative stress in plasma and liver, with the return of the tested parameters to normal values, 6 h after ip injection of nanotubes, with the exception of reduced glutathione in plasma. The inflammatory cytokines (TNF-α, IL-1β) had a similar pattern of evolution. We also assessed the level of ERK1/2 and the phosphorylation of p65 subunit of NF-kB in liver that had a transient increase and returned to normal at the end of the tested period. Our results demonstrate that ss-DNA-MWCNTs produce oxidative stress and inflammation, but with a transient pattern. Given the fact that antioxidants modify the profile not only for oxidative stress, but also of inflammation, the dynamics of these alterations may be of practical importance for future protective strategies. -- Highlights: ► ss-DNA-MWCNTs ip administration induce oxidative stress in plasma and liver. ► ss-DNA-MWCNTs ip administration determine liver inflammation. ► ERK1/2 and p65 phosphorylated NF-KB increase

  17. Community Assessment Tool for Public Health Emergencies Including Pandemic Influenza

    SciTech Connect (OSTI)

    HCTT-CHE

    2011-04-14

    The Community Assessment Tool (CAT) for Public Health Emergencies Including Pandemic Influenza (hereafter referred to as the CAT) was developed as a result of feedback received from several communities. These communities participated in workshops focused on influenza pandemic planning and response. The 2008 through 2011 workshops were sponsored by the Centers for Disease Control and Prevention (CDC). Feedback during those workshops indicated the need for a tool that a community can use to assess its readiness for a disaster—readiness from a total healthcare perspective, not just hospitals, but the whole healthcare system. The CAT intends to do just that—help strengthen existing preparedness plans by allowing the healthcare system and other agencies to work together during an influenza pandemic. It helps reveal each core agency partners' (sectors) capabilities and resources, and highlights cases of the same vendors being used for resource supplies (e.g., personal protective equipment [PPE] and oxygen) by the partners (e.g., public health departments, clinics, or hospitals). The CAT also addresses gaps in the community's capabilities or potential shortages in resources. While the purpose of the CAT is to further prepare the community for an influenza pandemic, its framework is an extension of the traditional all-hazards approach to planning and preparedness. As such, the information gathered by the tool is useful in preparation for most widespread public health emergencies. This tool is primarily intended for use by those involved in healthcare emergency preparedness (e.g., community planners, community disaster preparedness coordinators, 9-1-1 directors, hospital emergency preparedness coordinators). It is divided into sections based on the core agency partners, which may be involved in the community's influenza pandemic influenza response.

  18. Defects and persistent conductivity in SrTiO{sub 3}

    SciTech Connect (OSTI)

    McCluskey, Matthew D.; Tarun, Marianne C.

    2014-02-21

    Strontium titanate (SrTiO{sub 3}) is often used as a substrate for oxide thin films such as high-temperature superconductors. It has the perovskite structure and an indirect band gap of 3.25 eV. Our prior work showed that hydrogen impurities form a defect complex that contains two hydrogen atoms. The complex was tentatively attributed to a passivated strontium vacancy. Alternatively, it could be a partially passivated titanium vacancy. In order to create titanium vacancies, we annealed samples in an evacuated ampoule with SrO powder. These samples show unexpected behavior. After illuminating with sub-gap light, the free-electron concentration increases significantly. After the light is turned off, the high conductivity persists at room temperature. We attribute persistent photoconductivity (PPC) to the excitation of an electron from a vacancy into the conduction band, with a low recapture rate.

  19. A mesoporous metal–organic framework based on a shape-persistent macrocycle

    SciTech Connect (OSTI)

    Chen, Teng -Hao; Popov, Ilya; Chuang, Yu -Chun; Chen, Yu -Sheng; Miljani, Ognjen S.

    2015-08-26

    A mesoporous Zn-based metal–organic framework (MOF) was prepared from a shape-persistent phenylene ethynylene macrocycle functionalized with three –COOH groups. The rigid ligand has a ~ 9 Å wide central cavity which serves as a predesigned pore. The macrocycles [ππ] stack into pairs, with their carboxylate groups connected via three Zn₃O₁₄C₆H₂ clusters. The resulting MOF has a void volume of 86%.

  20. Persistent State-of-Charge Heterogeneity in Fully Relaxed Battery Electrode

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles | Stanford Synchrotron Radiation Lightsource Persistent State-of-Charge Heterogeneity in Fully Relaxed Battery Electrode Particles Friday, July 29, 2016 Figure. A transmission x-ray micrograph of an individual secondary particle charged to 30% global SOC at a 100 h rate, showing overcharged (green) domains even at this slow charging rate. Lithium ion batteries are used ubiquitously for portable energy storage in today's modern electronic devices and have served in that capacity for

  1. Studies of Nb3Sn Strands Based on the Restacked-Rod Process for High-Field Accelerator Magnets Nb3Sn

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Barzi, E; Bossert, M; Gallo, G; Lombardo, V; Turrioni, D; Yamada, R; Zlobin, A V

    2012-06-01

    A major thrust in Fermilab's accelerator magnet R&D program is the development of Nb3Sn wires which meet target requirements for high field magnets, such as high critical current density, low effective filament size, and the capability to withstand the cabling process. The performance of a number of strands with 150/169 restack design produced by Oxford Superconducting Technology was studied for round and deformed wires. To optimize the maximum plastic strain, finite element modeling was also used as an aid in the design. Results of mechanical, transport and metallographic analyses are presented for round and deformed wires.

  2. Persistence of gamma-H2AX and 53BP1 foci in proliferating and nonproliferating human mammary epithelial cells after exposure to gamma-rays or iron ions

    SciTech Connect (OSTI)

    Groesser, Torsten; Chang, Hang; Fontenay, Gerald; Chen, James; Costes, Sylvain V.; Barcellos-Hoff, Mary Helen; Parvin, Bahram; Rydberg, Bjorn

    2010-12-22

    To investigate {gamma}-H2AX (phosphorylated histone H2AX) and 53BP1 (tumour protein 53 binding protein No. 1) foci formation and removal in proliferating and non-proliferating human mammary epithelial cells (HMEC) after exposure to sparsely and densely ionizing radiation under different cell culture conditions. HMEC cells were grown either as monolayers (2D) or in extracellular matrix to allow the formation of acinar structures in vitro (3D). Foci numbers were quantified by image analysis at various time points after exposure. Our results reveal that in non-proliferating cells under 2D and 3D cell culture conditions, iron-ion induced {gamma}-H2AX foci were still present at 72 h after exposure, although 53BP1 foci returned to control levels at 48 h. In contrast in proliferating HMEC, both {gamma}-H2AX and 53BP1 foci decreased to control levels during the 24-48 h time interval after irradiation under 2D conditions. Foci numbers decreased faster after {gamma}-ray irradiation and returned to control levels by 12 h regardless of marker, cell proliferation status, and cell culture condition. Conclusions: The disappearance of radiation induced {gamma}-H2AX and 53BP1 foci in HMEC have different dynamics that depend on radiation quality and proliferation status. Notably, the general patterns do not depend on the cell culture condition (2D versus 3D). We speculate that the persistent {gamma}-H2AX foci in iron-ion irradiated non-proliferating cells could be due to limited availability of double strand break (DSB) repair pathways in G0/G1-phase, or that repair of complex DSB requires replication or chromatin remodeling.

  3. A functional role of Rv1738 in Mycobacterium tuberculosis persistence suggested by racemic protein crystallography

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bunker, Richard D.; Mandal, Kalyaneswar; Bashiri, Ghader; Chaston, Jessica J.; Pentelute, Bradley L.; Lott, J. Shaun; Kent, Stephen B. H.; Baker, Edward N.

    2015-04-07

    Racemic protein crystallography was used to determine the X-ray structure of the predicted Mycobacterium tuberculosis protein Rv1738, which had been completely recalcitrant to crystallization in its natural L-form. Native chemical ligation was used to synthesize both L-protein and D-protein enantiomers of Rv1738. Crystallization of the racemic {D-protein + L-protein} mixture was immediately successful. The resulting crystals diffracted to high resolution and also enabled facile structure determination because of the quantized phases of the data from centrosymmetric crystals. The X-ray structure of Rv1738 revealed striking similarity with bacterial hibernation factors, despite minimal sequence similarity. As a result, we predict that Rv1738,more » which is highly up-regulated in conditions that mimic the onset of persistence, helps trigger dormancy by association with the bacterial ribosome.« less

  4. Giant persistent photoconductivity in BaTiO{sub 3}/TiO{sub 2} heterostructures

    SciTech Connect (OSTI)

    Plodinec, Milivoj; anti?, Ana; Gajovi?, Andreja; Zavanik, Janez; ?eh, Miran

    2014-10-13

    The persistent photoconductivity (PPC) effect in nanotube arrays of barium titanate and TiO{sub 2} (BTO/TiO{sub 2}NT) was studied at room temperature under daylight illumination. The BTO/TiO{sub 2}NT heterostructures exhibited a giant PPC effect that was six orders of magnitude higher than the dark conductivity, followed by a slow relaxation for 3 h. The PPC in this material was explained by the existence of defects at the surfaces and the interfaces of the investigated heterostructures. The sample was prepared using a two-step synthesis: the anodization of a Ti-foil and a subsequent hydrothermal synthesis. The structural and electrical characteristics were studied by micro-Raman spectroscopy, field-emission-gun scanning electron microscopy, and impedance spectroscopy.

  5. Producing Persistent, High-Current, High-Duty-Factor H- Beams for Routine 1 MW Operation of SNS

    SciTech Connect (OSTI)

    Stockli, Martin P; Han, Baoxi; Hardek, Thomas W; Kang, Yoon W; Murray Jr, S N; Pennisi, Terry R; Piller, Chip; Santana, Manuel; Welton, Robert F

    2012-01-01

    Since 2009, SNS has been producing neutrons with ion beam powers near 1 MW, which requires the extraction of ~50 mA H- ions from the ion source with a ~5% duty factor. The 50 mA are achieved after an initial dose of ~3 mg of Cs and heating the Cs collar to ~170 C. The 50 mA normally persist for the entire 4-week source service cycles. Fundamental processes are reviewed to elucidate the persistence of the SNS H- beams without a steady feed of Cs and why the Cs collar temperature may have to be kept near 170 C.

  6. Insights from Smart Meters: Ramp Up, Dependability, and Short-Term Persistence of Savings from Home Energy Reports

    Broader source: Energy.gov [DOE]

    In this report, we use smart meter data to analyze the ramp-up, dependability, and short-term persistence of savings in one type of BB program: Home Energy Reports (HERs). In these programs, reports are mailed to households on a monthly, bi-monthly, or even quarterly basis. The reports provide energy tips and information about how a household's energy use compares to its neighbors. HERs typically obtain 1% to 3% annual electricity savings; several studies report that savings from mature HERs persist over multiple years while the programs are running (and decay after the reports are discontinued).

  7. ARM: Surface Radiation Measurement Quality Control testing, including climatologically configurable limits

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Shi, Yan; Riihimaki, Laura

    1994-01-07

    Surface Radiation Measurement Quality Control testing, including climatologically configurable limits

  8. Finite-size effects in a metallic multichannel ring with Kondo impurity: Persistent currents and magnetoresistance

    SciTech Connect (OSTI)

    Zvyagin, A.A.; Schlottmann, P.

    1996-12-01

    We consider a spin-1/2 impurity interacting with conduction electrons in two different orbital channels via an isotropic spin exchange. The exchange is the same for both channels, but a crystalline field breaks the symmetry between the orbital channels. This corresponds to a splitting of the conduction electron {Gamma}{sub 8} into two doublets in the quadrupolar Kondo effect, or to the electron-assisted tunneling of an atom in a double-well potential in an external magnetic field. Another possible realization could be a quantum dot coupled to two equal rings of the same length subject to an electrostatic potential difference. We consider the Bethe ansatz equations for this model and derive the tower structure of the finite-size corrections to the ground-state energy. These results are used to discuss the Aharonov-Bohm-Casher interference pattern in the persistent charge and spin currents, and the magnetoresistivity due to the scattering of electrons off the impurity. {copyright} {ital 1996 The American Physical Society.}

  9. General formulation of characteristic time for persistent chemicals in a multimedia environment

    SciTech Connect (OSTI)

    Bennett, D.H.; McKone, T.E.; Kastenberg, W.E.

    1999-02-01

    A simple yet representative method for determining the characteristic time a persistent organic pollutant remains in a multimedia environment is presented. The characteristic time is an important attribute for assessing long-term health and ecological impacts of a chemical. Calculating the characteristic time requires information on decay rates in multiple environmental media as well as the proportion of mass in each environmental medium. The authors explore the premise that using a steady-state distribution of the mass in the environment provides a means to calculate a representative estimate of the characteristic time while maintaining a simple formulation. Calculating the steady-state mass distribution incorporates the effect of advective transport and nonequilibrium effects resulting from the source terms. Using several chemicals, they calculate and compare the characteristic time in a representative multimedia environment for dynamic, steady-state, and equilibrium multimedia models, and also for a single medium model. They demonstrate that formulating the characteristic time based on the steady-state mass distribution in the environment closely approximates the dynamic characteristic time for a range of chemicals and thus can be used in decisions regarding chemical use in the environment.

  10. Analysis of Strand-Specific RNA-Seq Data Using Machine Learning Reveals the Structures of Transcription Units in Clostridium thermocellum

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chou, Wen-Chi; Ma, Qin; Yang, Shihui; Cao, Sha; Klingeman, Dawn M.; Brown, Steven D.; Xu, Ying

    2015-03-12

    The identification of transcription units (TUs) encoded in a bacterial genome is essential to elucidation of transcriptional regulation of the organism. To gain a detailed understanding of the dynamically composed TU structures, we have used four strand-specific RNA-seq (ssRNA-seq) datasets collected under two experimental conditions to derive the genomic TU organization of Clostridium thermocellum using a machine-learning approach. Our method accurately predicted the genomic boundaries of individual TUs based on two sets of parameters measuring the RNA-seq expression patterns across the genome: expression-level continuity and variance. A total of 2590 distinct TUs are predicted based on the four RNA-seq datasets.more » Moreover, among the predicted TUs, 44% have multiple genes. We assessed our prediction method on an independent set of RNA-seq data with longer reads. The evaluation confirmed the high quality of the predicted TUs. Functional enrichment analyses on a selected subset of the predicted TUs revealed interesting biology. To demonstrate the generality of the prediction method, we have also applied the method to RNA-seq data collected on Escherichia coli and achieved high prediction accuracies. The TU prediction program named SeqTU is publicly available athttps://code.google.com/p/seqtu/. We expect that the predicted TUs can serve as the baseline information for studying transcriptional and post-transcriptional regulation in C. thermocellum and other bacteria.« less