National Library of Energy BETA

Sample records for incandescent standard fluorescent

  1. 2014-04-11 Issuance: Energy Conservation Standards for General Service Fluorescent Lamps and Incandescent Reflector Lamps; Notice of Proposed Rulemaking

    Broader source: Energy.gov [DOE]

    This document is a pre-publication Federal Register notice of proposed rulemaking regarding energy conservation standards for general service fluorescent lamps and incandescent reflectors lamps, as issued by the Assistant Secretary for Energy Efficiency and Renewable Energy on April 11, 2014.

  2. ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen"

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: AlternativeMonthly","10/2015"Monthly","10/2015" ,"Release7 Relative Standard Errors for Table 5.7;" " Unit:8977.8.38.

  3. ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen"

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: AlternativeMonthly","10/2015"Monthly","10/2015" ,"Release7 Relative Standard Errors for Table 5.7;" "

  4. Potential Environmental Impacts from the Metals in Incandescent, Compact Fluorescent Lamp (CFL), and Light-Emitting Diode (LED)

    E-Print Network [OSTI]

    Short, Daniel

    Potential Environmental Impacts from the Metals in Incandescent, Compact Fluorescent Lamp (CFL, previous studies have investigated various environmental impacts from incandescent, CFL, and LED bulbs. There is uncertainty about the potential environmental impacts of these components and whether special provisions must

  5. How Energy-Efficient Light Bulbs Compare with Traditional Incandescent...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    you can save 75 each year. Compared to traditional incandescents, energy-efficient lightbulbs such as halogen incandescents, compact fluorescent lamps (CFLs), and light emitting...

  6. A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing ToolInternationalReportOffice | DepartmentVery1, in: Statement of Dr.26Former|A

  7. High efficiency incandescent lighting

    DOE Patents [OSTI]

    Bermel, Peter; Ilic, Ognjen; Chan, Walker R.; Musabeyoglu, Ahmet; Cukierman, Aviv Ruben; Harradon, Michael Robert; Celanovic, Ivan; Soljacic, Marin

    2014-09-02

    Incandescent lighting structure. The structure includes a thermal emitter that can, but does not have to, include a first photonic crystal on its surface to tailor thermal emission coupled to, in a high-view-factor geometry, a second photonic filter selected to reflect infrared radiation back to the emitter while passing visible light. This structure is highly efficient as compared to standard incandescent light bulbs.

  8. ECE 466: LED Lighting Systems -Incandescent lightings rise and

    E-Print Network [OSTI]

    Connors, Daniel A.

    ECE 466: LED Lighting Systems - Incandescent lightings rise and demise via government policy - Alternative Fluorescent light sources and compact fluorescent lights (CFL) to incandescents - Alternative LED light sources - Color index as well as Watts to Lumens efficiency available from all three light sources

  9. Dual LED/incandescent security fixture

    DOE Patents [OSTI]

    Gauna, Kevin Wayne

    2005-06-21

    A dual LED and incandescent security lighting system uses a hybrid approach to LED illumination. It combines an ambient LED illuminator with a standard incandescent lamp on a motion control sensor. The LED illuminator will activate with the onset of darkness (daylight control) and typically remain on during the course of the night ("always on"). The LED illumination, typically amber, is sufficient to provide low to moderate level lighting coverage to the wall and ground area adjacent to and under the fixture. The incandescent lamp is integrated with a motion control circuit and sensor. When movement in the field of view is detected (after darkness), the incandescent lamp is switched on, providing an increased level of illumination to the area. Instead of an "always on" LED illuminator, the LEDs may also be switched off when the incandescent lamp is switched on.

  10. Incandescent Lighting | Department of Energy

    Office of Environmental Management (EM)

    courtesy of iStockphotoTokenPhoto. Incandescent lamps are often considered the least energy efficient type of electric lighting commonly found in residential buildings....

  11. APPLIANCE STANDARDS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Equipment General Service Incandescent Lamps General Service Fluorescent Lamps Small Electric Motors Electric Motors Water Heaters Furnace Fans Distribution Transformers Water...

  12. Fluorescent Lighting | Department of Energy

    Office of Environmental Management (EM)

    - by far the most common form of fluorescent lighting but rarely found in residential buildings -- are much more energy efficient than incandescent lamps and are ideally suited...

  13. How Energy-Efficient Light Bulbs Compare with Traditional Incandescent...

    Office of Environmental Management (EM)

    Energy-Efficient Light Bulbs Compare with Traditional Incandescents How Energy-Efficient Light Bulbs Compare with Traditional Incandescents November 5, 2014 - 11:39pm Addthis By...

  14. ISSUANCE 2014-12-29: Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

    Broader source: Energy.gov [DOE]

    Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

  15. Max Tech Appliance Design: Potential for Maximizing U.S. Energy Savings through Standards

    E-Print Network [OSTI]

    Garbesi, Karina

    2011-01-01

    fluorescent and lighting dominantly incandescent. LED)LED incandescent efficacies are expected to increase lightingLED Heat pump Heat pump Heat pump d Energy Use (TWh) a Lighting (

  16. EA-1881: Energy Conservation Program: Energy Conservation Standards for Fluorescent Lamp Ballasts

    Broader source: Energy.gov [DOE]

    This EA evaluates the environmental impacts of a proposal to amend energy conservation standards for various consumer products and certain commercial and industrial equipment, including fluorescent lamp ballasts.

  17. A light diet for a giant appetite: An assessment of China's proposed fluorescent lamp standard

    SciTech Connect (OSTI)

    Lin, Jiang

    2002-04-11

    Lighting has been one of the fastest growing electric end-uses in China over the last twenty years, with an average annual growth rate of 14%. Fluorescent lighting provides a significant portion of China's lighting need. In 1998, China produced 680 million fluorescent lamps, of which 420 million were linear fluorescent lamps of various diameters (T8 to T12). There are substantial variations both in energy efficiency and lighting performance among locally produced fluorescent lamps. Such variations present a perfect opportunity for policy intervention through efficiency standards to promote the adoption of more efficient fluorescent lamps in China. This paper analyzes China's proposed minimum efficiency standard for fluorescent lamps and presents an assessment of its likely impacts on China's lighting energy consumption and GHG emissions.

  18. How Energy-Efficient Light Bulbs Compare with Traditional Incandescent...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    used light fixtures or bulbs with models that have earned the ENERGY STAR, you can save 75 each year. Compared to traditional incandescents, energy-efficient lightbulbs...

  19. EA-1664: Final Environmental Assessment

    Broader source: Energy.gov [DOE]

    10 CFR Part 430 Energy Conservation Standards: Energy Conservation Standards for Fluorescent and Incandescent Lamps

  20. ISSUANCE 2015-06-17: Energy Conservation Standards for Fluorescent Lamp Ballasts, Notice of Public Meeting and Availability of the Framework Document

    Office of Energy Efficiency and Renewable Energy (EERE)

    Energy Conservation Standards for Fluorescent Lamp Ballasts, Notice of Public Meeting and Availability of the Framework Document

  1. ISSUANCE 2015-08-14: Energy Efficiency Program for Consumer Products: Energy Conservation Standards for Fluorescent Lamp Ballasts, Reopening of the Comment Period

    Broader source: Energy.gov [DOE]

    Energy Efficiency Program for Consumer Products: Energy Conservation Standards for Fluorescent Lamp Ballasts, Reopening of the Comment Period

  2. New Lighting Standards Began in 2012 | Department of Energy

    Office of Environmental Management (EM)

    save you about 50 per year when you replace 15 traditional incandescent bulbs in your home. Measuring Light in Lumens The new efficiency standards require lightbulbs to consume...

  3. Standard test method for determination of uranium or gadolinium (or both) in gadolinium oxide-uranium oxide pellets or by X-ray fluorescence (XRF)

    E-Print Network [OSTI]

    American Society for Testing and Materials. Philadelphia

    2008-01-01

    Standard test method for determination of uranium or gadolinium (or both) in gadolinium oxide-uranium oxide pellets or by X-ray fluorescence (XRF)

  4. New Homogeneous Standards by Atomic Layer Deposition for Synchrotron X-ray Fluorescence and Absorption Spectroscopies.

    SciTech Connect (OSTI)

    Butterworth, A.L.; Becker, N.; Gainsforth, Z.; Lanzirotti, A.; Newville, M.; Proslier, T.; Stodolna, J.; Sutton, S.; Tyliszczak, T.; Westphal, A.J.; Zasadzinski, J. (UCB)

    2012-03-13

    Quantification of synchrotron XRF analyses is typically done through comparisons with measurements on the NIST SRM 1832/1833 thin film standards. Unfortunately, these standards are inhomogeneous on small scales at the tens of percent level. We are synthesizing new homogeneous multilayer standards using the Atomic Layer Deposition technique and characterizing them using multiple analytical methods, including ellipsometry, Rutherford Back Scattering at Evans Analytical, Synchrotron X-ray Fluorescence (SXRF) at Advanced Photon Source (APS) Beamline 13-ID, Synchrotron X-ray Absorption Spectroscopy (XAS) at Advanced Light Source (ALS) Beamlines 11.0.2 and 5.3.2.1 and by electron microscopy techniques. Our motivation for developing much-needed cross-calibration of synchrotron techniques is borne from coordinated analyses of particles captured in the aerogel of the NASA Stardust Interstellar Dust Collector (SIDC). The Stardust Interstellar Dust Preliminary Examination (ISPE) team have characterized three sub-nanogram, {approx}1{micro}m-sized fragments considered as candidates to be the first contemporary interstellar dust ever collected, based on their chemistries and trajectories. The candidates were analyzed in small wedges of aerogel in which they were extracted from the larger collector, using high sensitivity, high spatial resolution >3 keV synchrotron x-ray fluorescence spectroscopy (SXRF) and <2 keV synchrotron x-ray transmission microscopy (STXM) during Stardust ISPE. The ISPE synchrotron techniques have complementary capabilities. Hard X-ray SXRF is sensitive to sub-fg mass of elements Z {ge} 20 (calcium) and has a spatial resolution as low as 90nm. X-ray Diffraction data were collected simultaneously with SXRF data. Soft X-ray STXM at ALS beamline 11.0.2 can detect fg-mass of most elements, including cosmochemically important oxygen, magnesium, aluminum and silicon, which are invisible to SXRF in this application. ALS beamline 11.0.2 has spatial resolution better than 25 nm. Limiting factors for Stardust STXM analyses were self-imposed limits of photon dose due to radiation damage concerns, and significant attenuation of <1500 eV X-rays by {approx}80{micro}m thick, {approx}25 mg/cm{sup 3} density silica aerogel capture medium. In practice, the ISPE team characterized the major, light elements using STXM (O, Mg, Al, Si) and the heavier minor and trace elements using SXRF. The two data sets overlapped only with minor Fe and Ni ({approx}1% mass abundance), providing few quantitative cross-checks. New improved standards for cross calibration are essential for consortium-based analyses of Stardust interstellar and cometary particles, IDPs. Indeed, they have far reaching application across the whole synchrotron-based analytical community. We have synthesized three ALD multilayers simultaneously on silicon nitride membranes and silicon and characterized them using RBS (on Si), XRF (on Si{sub 3}N{sub 4}) and STXM/XAS (holey Si{sub 3}N{sub 4}). The systems we have started to work with are Al-Zn-Fe and Y-Mg-Er. We have found these ALD multi-layers to be uniform at {micro}m- to nm scales, and have found excellent consistency between four analytical techniques so far. The ALD films can also be used as a standard for e-beam instruments, eg., TEM EELS or EDX. After some early issues with the consistency of coatings to the back-side of the membrane windows, we are confident to be able to show multi-analytical agreement to within 10%. As the precision improves, we can use the new standards to verify or improve the tabulated cross-sections.

  5. CALiPER Benchmark Report: Performance of Incandescent A Type and Decorative Lamps and LED Replacements

    SciTech Connect (OSTI)

    None

    2008-11-01

    This benchmark report addresses common omnidirectional incandescent lamps - A-type and small decorative, candelabra-type lamps - and their commercially available light-emitting diode (LED) replacements.

  6. Standard test method for uranium analysis in natural and waste water by X-ray fluorescence

    E-Print Network [OSTI]

    American Society for Testing and Materials. Philadelphia

    2004-01-01

    1.1 This test method applies for the determination of trace uranium content in waste water. It covers concentrations of U between 0.05 mg/L and 2 mg/L. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

  7. Compact Fluorescent Plug-In Ballast-in-a-Socket

    SciTech Connect (OSTI)

    Rebecca Voelker

    2001-12-21

    The primary goal of this program was to develop a ballast system for plug-in CFLs (compact fluorescent lamps) that will directly replace standard metal shell, medium base incandescent lampholders (such as Levition No. 6098) for use with portable lamp fixtures, such as floor, table and desk lamps. A secondary goal was to identify a plug-in CFL that is optimized for use with this ballast. This Plug-in CFL Ballastin-a-Socket system will allow fixture manufacturers to easily manufacture CFL-based high-efficacy portable fixtures that provide residential and commercial consumers with attractive, cost-effective, and energy-efficient fixtures for use wherever portable incandescent fixtures are used today. The advantages of this proposed system over existing CFL solutions are that the fixtures can only be used with high-efficacy CFLs, and they will be more attractive and will have lower life-cycle costs than screw-in or adapter-based CFL retrofit solutions. These features should greatly increase the penetration of CFL's into the North American market. Our work has shown that using integrated circuits it is quite feasible to produce a lamp-fixture ballast of a size comparable to the current Edison-screw 3-way incandescent fixtures. As for price points for BIAS-based fixtures, end-users polled by the Lighting Research Institute at RPI indicated that they would pay as much as an additional $10 for a lamp containing such a ballast. The ballast has been optimized to run with a 26 W amalgam triple biax lamp in the base-down position, yet can accept non-amalgam versions of the lamp. With a few part alterations, the ballast can be produced to support 32 W lamps as well. The ballast uses GE's existing L-Comp[1] power topology in the circuit so that the integrated circuit design would be a design that could possibly be used by other CFL and EFL products with minor modifications. This gives added value by reducing cost and size of not only the BIAS, but also possibly other integral CFL and future dimmable integral and plug-in versions of the EFL products.

  8. Fluorescent Tube Lamps

    Broader source: Energy.gov [DOE]

    FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

  9. >.........standard

    E-Print Network [OSTI]

    to the multimedia research and product development industry Planned Accomplishments MPEG Standards: ·Establishing 2D. ......... IETF standard protocols Collaborators / Customers Standards Groups: MPEG, SMPTE NIST Collaborators: ATP Other Collaborators: Academic

  10. Evaluation of the irising effect of a slow-gating intensified charge-coupled device on laser-induced incandescence measurements of soot

    SciTech Connect (OSTI)

    Shaddix, Christopher R.; Williams, Timothy C. [Combustion Research Facility, Sandia National Laboratories, 7011 East Avenue, Livermore, California 94550 (United States)

    2009-03-15

    Intensified charge-coupled devices (ICCDs) are used extensively in many scientific and engineering environments to image weak or temporally short optical events. To optimize the quantum efficiency of light collection, many of these devices are chosen to have characteristic intensifier gate times that are relatively slow, on the order of tens of nanoseconds. For many measurements associated with nanosecond laser sources, such as scattering-based diagnostics and most laser-induced fluorescence applications, the signals rise and decay sufficiently fast during and after the laser pulse that the intensifier gate may be set to close after the cessation of the signal and still effectively reject interferences associated with longer time scales. However, the relatively long time scale and complex temporal response of laser-induced incandescence (LII) of nanometer-sized particles (such as soot) offer a difficult challenge to the use of slow-gating ICCDs for quantitative measurements. In this paper, ultraviolet Rayleigh scattering imaging is used to quantify the irising effect of a slow-gating scientific ICCD camera, and an analysis is conducted of LII image data collected with this camera as a function of intensifier gate width. The results demonstrate that relatively prompt LII detection, generally desirable to minimize the influences of particle size and local gas pressure and temperature on measurements of the soot volume fraction, is strongly influenced by the irising effect of slow-gating ICCDs.

  11. Technical support document: Energy efficiency standards for consumer products: Room air conditioners, water heaters, direct heating equipment, mobile home furnaces, kitchen ranges and ovens, pool heaters, fluorescent lamp ballasts and television sets. Volume 1, Methodology

    SciTech Connect (OSTI)

    Not Available

    1993-11-01

    The Energy Policy and Conservation Act (P.L. 94-163), as amended, establishes energy conservation standards for 12 of the 13 types of consumer products specifically covered by the Act. The legislation requires the Department of Energy (DOE) to consider new or amended standards for these and other types of products at specified times. DOE is currently considering amending standards for seven types of products: water heaters, direct heating equipment, mobile home furnaces, pool heaters, room air conditioners, kitchen ranges and ovens (including microwave ovens), and fluorescent light ballasts and is considering establishing standards for television sets. This Technical Support Document presents the methodology, data, and results from the analysis of the energy and economic impacts of the proposed standards. This volume presents a general description of the analytic approach, including the structure of the major models.

  12. Acting Globally: Potential Carbon Emissions Mitigation Impacts from an International Standards and Labelling Program

    SciTech Connect (OSTI)

    McNeil, Michael A; Letschert, Virginie E.; de la Rue du Can, Stephane; Egan, Christine

    2009-05-29

    This paper presents an analysis of the potential impacts of an international initiative designed to support and promote the development and implementation of appliances standards and labelling programs throughout the world. As part of previous research efforts, LBNL developed the Bottom Up Energy Analysis System (BUENAS), an analysis framework that estimates impact potentials of energy efficiency policies on a global scale. In this paper, we apply this framework to an initiative that would result in the successful implementation of programs focused on high priority regions and product types, thus evaluating the potential impacts of such an initiative in terms of electricity savings and carbon mitigation in 2030. In order to model the likely parameters of such a program, we limit impacts to a five year period starting in 2009, but assume that the first 5 years of a program will result in implementation of 'best practice' minimum efficiency performance standards by 2014. The 'high priority' regions considered are: Brazil, China, the European Union,India, Mexico and the United States. The products considered are: refrigerators, air conditioners, lighting (both fluorescent and incandescent), standby power (for consumer electronics) and televisions in the residential sector, and air conditioning and lighting in commercial buildings. In 2020, these regions and enduses account for about 37percent of global residential electricity and 29percent of electricity in commercial buildings. We find that 850Mt of CO2 could be saved in buildings by 2030 compared to the baseline forecast.

  13. Energy Savings and NOx Emissions Reduction Potential from the 2012 Federal Legislation to Phase Out Incandescent Lamps in Texas 

    E-Print Network [OSTI]

    Liu, Zi; Baltazar, Juan Carlos; Haberl, Jeff; Soman, Rohit

    2010-01-01

    Lamps in Texas Description Value Reference Total Housing Units in Texas in 2013: 10,204,056 Real Estate Center, Texas A&M University3, U.S. Census Bureau4 5 Average Lighting Electricity Usage per House: 1,946 kWh/yr NREL Building America Program6... % of Lamps That can be Retrofitted with a CFL: 70% NREL Building America Program6 and ESL Assumption % of Savings From Replacing Incandescent to CFL Lamps: 75% ESL Assumption CFL Lighting Electricity Savings per House: 1,022 kWh/yr Total Annual CFL...

  14. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, Richard I. (Santa Fe, NM); Edwards, Bradley C. (Los Alamos, NM); Buchwald, Melvin I. (Santa Fe, NM); Gosnell, Timothy R. (Santa Fe, NM)

    1995-01-01

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement.

  15. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, R.I.; Edwards, B.C.; Buchwald, M.I.; Gosnell, T.R.

    1995-09-05

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement. 6 figs.

  16. Standard test method for determination of bromine and chlorine in UF6 and uranyl nitrate by X-Ray fluorescence (XRF) spectroscopy

    E-Print Network [OSTI]

    American Society for Testing and Materials. Philadelphia

    2001-01-01

    1.1 This method covers the determination of bromine (Br) and chlorine (Cl) in uranium hexafluoride (UF6) and uranyl nitrate solution. The method as written covers the determination of bromine in UF6 over the concentration range of 0.2 to 8 ?g/g, uranium basis. The chlorine in UF6 can be determined over the range of 4 to 160 ?g/g, uranium basis. Higher concentrations may be covered by appropriate dilutions. The detection limit for Br is 0.2 ?g/g uranium basis and for Cl is 4 ?g/g uranium basis. 1.2 This standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

  17. Engineering novel fluorescent proteins

    E-Print Network [OSTI]

    Shaner, Nathan Christopher

    2006-01-01

    to Choosing Fluorescent Proteins. Nat. Methods. 2 (12): 905-Dynamics of Z-band based proteins in developing skeletaland yellow fluorescent proteins derived from Discosoma sp.

  18. Effects of aggregate morphology and size on laser-induced incandescence and scattering from black carbon (mature soot)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bambha, Ray P.; Michelsen, Hope A.

    2015-07-03

    We have used a Single-Particle Soot Photometer (SP2) to measure time-resolved laser-induced incandescence (LII) and laser scatter from combustion-generated mature soot with a fractal dimension of 1.88 extracted from a burner. We have also made measurements on restructured mature-soot particles with a fractal dimension of 2.3–2.4. We reproduced the LII and laser-scatter temporal profiles with an energy- and mass-balance model, which accounted for heating of particles passed through a CW-laser beam over laser–particle interaction times of ~10 ?s. Furthermore, the results demonstrate a strong influence of aggregate size and morphology on LII and scattering signals. Conductive cooling competes with absorptivemore »heating on these time scales; the effects are reduced with increasing aggregate size and fractal dimension. These effects can lead to a significant delay in the onset of the LII signal and may explain an apparent low bias in the SP2 measurements for small particle sizes, particularly for fresh, mature soot. The results also reveal significant perturbations to the measured scattering signal from LII interference and suggest rapid expansion of the aggregates during sublimation.« less

  19. Soot particle sizing during high-pressure Diesel spray combustion via time-resolved laser-induced incandescence

    SciTech Connect (OSTI)

    Ryser, R.; Gerber, T.; Dreier, T. [Reaction Analysis Group, Department of General Energy, Paul Scherrer Institute, CH-5232 Villigen (Switzerland)

    2009-01-15

    Single-pulse time-resolved laser-induced incandescence (TiRe-LII) signal transients from soot particulates were acquired during unsteady high pressure Diesel combustion in a constant volume cell for typical top dead center conditions during a Diesel engine cycle. Measurements were performed for initial gas pressures between 1 and 3 MPa, injection pressures between 50 and 130 MPa and laser probe timings between 5 and 16 ms after start of fuel injection. In separate experiments and for the same cell operating conditions gas temperatures were deduced from spectrally resolved soot pyrometry measurements. Implementing the LII model of Kock et al. [Combust. Flame 147 (20006) 79-92] ensemble mean soot particle diameters were evaluated from least-squares fitting of theoretical cooling curves to experimental TiRe-LII signal transients. Since in the experiments the environmental gas temperature and the width of an assumed particle size distribution were not known, the effects of the initial choice of these parameters on retrieved particle diameters were investigated. It is shown that evaluated mean particle diameters are only slightly biased by the choice of typical size distribution widths and gas temperatures. For a fixed combustion phase mean particle diameters are not much affected by gas pressure, however they become smaller at high fuel injection pressure. At a mean chamber pressure of 1.39 MPa evaluated mean particle diameters increased by a factor of two for probe delays between 5 and 16 ms after start of injection irrespective of the choices of first-guess fitting variables, indicating a certain robustness of data analysis procedure. (author)

  20. Soot particle sizing during high-pressure Diesel spray combustion via time-resolved laser-induced incandescence

    SciTech Connect (OSTI)

    Ryser, R.; Gerber, T.; Dreier, T. [Reaction Analysis Group, Department of General Energy, Paul Scherrer Institute, CH-5232 Villigen PSI (Switzerland)

    2008-11-15

    Single-pulse time-resolved laser-induced incandescence (TiRe-LII) signal transients from soot particulates were acquired during unsteady high pressure Diesel combustion in a constant volume cell for typical top dead center conditions during a Diesel engine cycle. Measurements were performed for initial gas pressures between 1 and 3 MPa, injection pressures between 50 and 130 MPa and laser probe timings between 5 and 16 ms after start of fuel injection. In separate experiments and for the same cell operating conditions gas temperatures were deduced from spectrally resolved soot pyrometry measurements. Implementing the LII model of Kock et al. [Combust. Flame 147 (2006) 79-92] ensemble mean soot particle diameters were evaluated from least-squares fitting of theoretical cooling curves to experimental TiRe-LII signal transients. Since in the experiments the environmental gas temperature and the width of an assumed particle size distribution were not known, the effects of the initial choice of these parameters on retrieved particle diameters were investigated. It is shown that evaluated mean particle diameters are only slightly biased by the choice of typical size distribution widths and gas temperatures. For a fixed combustion phase mean particle diameters are not much affected by gas pressure, however they become smaller at high fuel injection pressure. At a mean chamber pressure of 1.39 MPa evaluated mean particle diameters increased by a factor of two for probe delays between 5 and 16 ms after start of injection irrespective of the choices of first-guess fitting variables, indicating a certain robustness of data analysis procedure. (author)

  1. Fluorescent optical position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  2. Safe biodegradable fluorescent particles

    DOE Patents [OSTI]

    Martin, Sue I. (Berkeley, CA); Fergenson, David P. (Alamo, CA); Srivastava, Abneesh (Santa Clara, CA); Bogan, Michael J. (Dublin, CA); Riot, Vincent J. (Oakland, CA); Frank, Matthias (Oakland, CA)

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  3. Sustainable LED Fluorescent Light Replacement Technology

    SciTech Connect (OSTI)

    2011-06-30

    Ilumisys and the National Center for Manufacturing Sciences (NCMS) partnered on a three-year project awarded by the United States (U.S.) Department of Energy (DOE), to quantify the impacts of LED lamps, incandescent lamps and fluorescent benchmark lamps over a product lifecycle – i.e. to develop a sustainable design and manufacturing strategy that addresses product manufacturing, use, recycling and disposal scenarios for LED-based lighting. Based on the knowledge gained from extensive product tear-down studies of fluorescent and screw-in lighting products, lifecycle assessment tools, and accelerated lifecycle testing protocols, an interactive Sustainable LED Design Guide has been developed to aid architectural and lighting designers and engineers in making design decisions that consider three important environmental impacts (greenhouse gas emissions, energy use and mercury emission) across all phases of the life of an LED lighting product. Critical information developed for the lifecycle analysis and product feature comparisons is the useful life of the lighting product as well as its performance. The Design Guide is available at www.ncms.org, and was developed based on operational and durability testing of a variety of lighting products including power consumption, light output, and useful life of a lamp in order to allow a more realistic comparison of lamp designs. This report describes the main project tasks, results and innovative features of the lifecycle assessment (LCA)-based design tools, and the key considerations driving the sustainable design of LED lighting systems. The Design Guide incorporates the following three novel features for efficiently evaluating LED lighting features in value-chains: • Bill-of-Materials (BOM) Builder – Designers may import process data for each component and supply functional data for the product, including power, consumption, lumen output and expected useful life. • Environmental Impact Review – Designs are comparable across lifecycle phases, subsystems, and environmental impact category, and can be normalized to a userdefined functional unit. • Drill-down Review – These provide an indepth look at individual lamp designs with the ability to review across subsystem or lifecycle phase.

  4. Photo: D. Stevenson and C. Conway/Beckman Institute/University of Illinois An inorganic LED display printed on a flexible substrate bends without breaking

    E-Print Network [OSTI]

    Rogers, John A.

    cameras. Conventional inorganic LEDs, which are poised to put incandescent and fluorescent lightbulbs out

  5. Initial Score: # of workers

    E-Print Network [OSTI]

    Yamamoto, Keith

    My lab has replaced incandescent lamps with Compact Fluorescent Lamps (CFLs) or Light Emitting Diodes

  6. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M. (Santa Fe, NM); Waldo, Geoffrey S. (Santa Fe, NM); Kiss, Csaba (Los Alamos, NM)

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  7. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M. (Santa Fe, NM); Waldo, Geoffrey S. (Santa Fe, NM); Kiss, Csaba (Los Alamos, NM)

    2011-11-29

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  8. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M. (Santa Fe, NM); Waldo, Geoffrey S. (Santa Fe, NM); Kiss, Csaba (Los Alamos, NM)

    2012-05-01

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  9. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen R. (Princeton, NJ); Sun, Yiru (Princeton, NJ); Giebink, Noel (Princeton, NJ); Thompson, Mark E. (Anaheim Hills, CA)

    2009-01-06

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  10. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen (Ann Arbor, MI); Sun, Yiru (Princeton, NJ); Giebink, Noel (Ann Arbor, MI); Thompson, Mark E. (Anaheim Hills, CA)

    2010-08-03

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  11. Fluorescent image tracking velocimeter

    DOE Patents [OSTI]

    Shaffer, Franklin D. (Library, PA)

    1994-01-01

    A multiple-exposure fluorescent image tracking velocimeter (FITV) detects and measures the motion (trajectory, direction and velocity) of small particles close to light scattering surfaces. The small particles may follow the motion of a carrier medium such as a liquid, gas or multi-phase mixture, allowing the motion of the carrier medium to be observed, measured and recorded. The main components of the FITV include: (1) fluorescent particles; (2) a pulsed fluorescent excitation laser source; (3) an imaging camera; and (4) an image analyzer. FITV uses fluorescing particles excited by visible laser light to enhance particle image detectability near light scattering surfaces. The excitation laser light is filtered out before reaching the imaging camera allowing the fluoresced wavelengths emitted by the particles to be detected and recorded by the camera. FITV employs multiple exposures of a single camera image by pulsing the excitation laser light for producing a series of images of each particle along its trajectory. The time-lapsed image may be used to determine trajectory and velocity and the exposures may be coded to derive directional information.

  12. Technical support document: Energy efficiency standards for consumer products: Room air conditioners, water heaters, direct heating equipment, mobile home furnaces, kitchen ranges and ovens, pool heaters, fluorescent lamp ballasts and television sets. Volume 2, Fluorescent lamp ballasts, television sets, room air conditioners, and kitchen ranges and ovens

    SciTech Connect (OSTI)

    Not Available

    1993-11-01

    This document is divided into ``volumes`` B through E, dealing with individual classes of consumer products. Chapters in each present engineering analysis, base case forecasts, projected national impacts of standards, life-cycle costs and payback periods, impacts on manufacturers, impacts of standards on electric utilities, and environmental effects. Supporting appendices are included.

  13. Replacing Fluorescent Lightbulbs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lightbulbs Replacing Fluorescent Lightbulbs July 29, 2012 - 6:37pm Addthis Although fluorescent lightbulbs are generally energy efficient, you can replace them with...

  14. Fluorescent Protein Applications in Microscopy

    E-Print Network [OSTI]

    Straight, Aaron

    . The Identification of Green Fluorescent Protein III. Formation of the GFP Chromophore IV. The Structure of GFP V environment. II. The Identification of Green Fluorescent Protein The isolation of green fluorescent protein of Aequorea, Shimomura et al. noted that the lumines- cence from aequorin was blue rather than the green

  15. The Air-Fluorescence Yield

    E-Print Network [OSTI]

    F. Arqueros; F. Blanco; D. Garcia-Pinto; M. Ortiz; J. Rosado

    2008-07-30

    Detection of the air-fluorescence radiation induced by the charged particles of extensive air showers is a well-established technique for the study of ultra-high energy cosmic rays. Fluorescence telescopes provide a nearly calorimetric measure of the primary energy. Presently the main source of systematic uncertainties comes from our limited accuracy in the fluorescence yield, that is, the number of fluorescence photons emitted per unit of energy deposited in the atmosphere by the shower particles. In this paper the current status of our knowledge on the fluorescence yield both experimental an theoretical will be discussed.

  16. Magnetic fluorescent lamp

    DOE Patents [OSTI]

    Berman, S.M.; Richardson R.W.

    1983-12-29

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  17. Optimizing Energy Savings from Direct-DC in U.S. Residential Buildings

    E-Print Network [OSTI]

    Garbesi, Karina

    2012-01-01

    ballast fluorescent and LED lighting, which are DC- internalmotors [7]. Fluorescent and LED lighting uses one-quarter ofbest technology Lighting Incandescent, fluorescent, LED

  18. Optimizing Energy Savings from Direct-DC in U.S. Residential Buildings

    E-Print Network [OSTI]

    Garbesi, Karina

    2012-01-01

    motors [7]. Fluorescent and LED lighting uses one-quarter ofballast fluorescent and LED lighting, which are DC- internalbest technology Lighting Incandescent, fluorescent, LED

  19. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Dougalas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2003-12-09

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  20. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Douglas C. (San Jose, CA); Silva, Christopher M. (Sunnyvale, CA); Bylina, Edward J. (San Jose, CA); Coleman, William J. (Moutain View, CA); Dilworth, Michael R. (Santa Cruz, CA); Yang, Mary M. (San Jose, CA)

    2002-09-24

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  1. Luminous Efficacy Standards for General Purpose Lights

    Broader source: Energy.gov [DOE]

    *Efficacy refers to the overall energy efficiency of light and is measured in lumens (measure of light output) per watt (measure of power input). The efficacy of a typical incandescent light bulb...

  2. Fluorescence analyzer for lignin

    DOE Patents [OSTI]

    Berthold, John W. (Salem, OH); Malito, Michael L. (Hubbard, OH); Jeffers, Larry (Alliance, OH)

    1993-01-01

    A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

  3. Fluorescent temperature sensor

    DOE Patents [OSTI]

    Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

    2009-03-03

    The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

  4. Laser-Stimulated Fluorescence in Paleontology

    E-Print Network [OSTI]

    Kaye, Thomas G.; Falk, Amanda Renee; Pittman, Michael; Sereno, Paul C.; Martin, Larry D.; Burnham, David A.; Gong, Enpu; Xu, Xing; Wang, Yinan

    2015-05-27

    Fluorescence using ultraviolet (UV) light has seen increased use as a tool in paleontology over the last decade. Laser-stimulated fluorescence (LSF) is a next generation technique that is emerging as a way to fluoresce paleontological specimens...

  5. Design strategies for fluorescent biodegradable polymeric biomaterials

    E-Print Network [OSTI]

    Yang, Jian

    Design strategies for fluorescent biodegradable polymeric biomaterials Yi Zhangab and Jian Yang biomaterials: biodegradable fluorescent polymers. Researchers have made significant efforts in developing versatile fluorescent biomaterials due to their promising applications in biological/biomedical labeling

  6. Incandescent Lighting | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergy A plug-inPPLforLDRD Report11,SecurityHomeRemarks As Prepared for

  7. Incandescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuelsof Energy ServicesContractingManagementSuccess,Energy

  8. Nuclear Resonance Fluorescence for Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian

    2010-01-01

    clandestine material with nuclear resonance fluorescence”.E. Norman, UC Berkeley Dept. of Nuclear Engineering, privatepp. 349. G. Warren et al. “Nuclear Resonance Fluorescence of

  9. Got Standards? "Got Standards?"

    E-Print Network [OSTI]

    Vardeman, Stephen B.

    or organization chooses to manage their activities. Obtaining ISO 9000 Certification Implementing the ISO 9000, assuming the company complies with the standard of ISO 9000. The ISO 9000 certificate will usually expire 9000 While many companies try to obtain ISO 9000 certification, there are many other different ISO

  10. Nuclear Resonance Fluorescence for Nuclear Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian Joseph

    2010-01-01

    Potential of Nuclear Resonance Fluorescence . . . . . . . .2.9.1 Nuclear ThomsonSections . . . . . . . . . . . . . . . Nuclear Resonance

  11. Product Standards for Fluorescent Lighting (Japan) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsourceII JumpQuarterly SmartDB-2, BluePoulsenBioSolutions JumpProblems

  12. APPLIANCE STANDARDS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    APPLIANCE STANDARDS How they interact with energy efficiency programs Alas, those mercurial baselines. Since 2005, 45 mandatory Department of Energy (DOE) efficiency standards have...

  13. Furnace Standard Analysis Discussion Document

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12, 2015ExecutiveFluorescentDanKathyEnergydetailsof EnergyStandard *

  14. Concentration dependence of fluorescence signal in a microfluidic fluorescence detector

    E-Print Network [OSTI]

    Klotzkin, David

    ] demonstrated a compact device using an OLED as light source and an integrated p-i-n photodiode as detector Available online 6 February 2010 Keywords: Lab-on-a-chip On-chip fluorescence Cross-polarization OLED OPD et al. [3] have recently demonstrated the use of a polyfluorene based pLED (polymer LED) as light

  15. Quantitative Modeling and Estimation in Systems Biology using Fluorescent Reporter Systems 

    E-Print Network [OSTI]

    Bansal, Loveleena

    2013-12-10

    FIM Fischer Information Matrix FP Fluorescent Protein GFP Green Fluorescent Protein GLS Generalized Least Squares IBVP Intital Boundary Value Problem IL-6 Interleukin-6 IPDE Integro Partial Differential Equation LHS Latin Hypercube... Sampling MSE Mean Squared Error nM Nano Molar O.D. Optical Density ODE Ordinary Differential Equation PBE Population Balance Equation PBM Population Balance Model RE Relative Error S.D. Standard Deviation STAT3 Signal transducer...

  16. Studies of the laser-induced fluorescence of explosives and explosive compositions.

    SciTech Connect (OSTI)

    Hargis, Philip Joseph, Jr.; Thorne, Lawrence R.; Phifer, Carol Celeste; Parmeter, John Ethan; Schmitt, Randal L.

    2006-10-01

    Continuing use of explosives by terrorists throughout the world has led to great interest in explosives detection technology, especially in technologies that have potential for standoff detection. This LDRD was undertaken in order to investigate the possible detection of explosive particulates at safe standoff distances in an attempt to identify vehicles that might contain large vehicle bombs (LVBs). The explosives investigated have included the common homogeneous or molecular explosives, 2,4,6-trinitrotoluene (TNT), pentaerythritol tetranitrate (PETN), cyclonite or hexogen (RDX), octogen (HMX), and the heterogeneous explosive, ammonium nitrate/fuel oil (ANFO), and its components. We have investigated standard excited/dispersed fluorescence, laser-excited prompt and delayed dispersed fluorescence using excitation wavelengths of 266 and 355 nm, the effects of polarization of the laser excitation light, and fluorescence imaging microscopy using 365- and 470-nm excitation. The four nitro-based, homogeneous explosives (TNT, PETN, RDX, and HMX) exhibit virtually no native fluorescence, but do exhibit quenching effects of varying magnitude when adsorbed on fluorescing surfaces. Ammonium nitrate and fuel oil mixtures fluoresce primarily due to the fuel oil, and, in some cases, due to the presence of hydrophobic coatings on ammonium nitrate prill or impurities in the ammonium nitrate itself. Pure ammonium nitrate shows no detectable fluorescence. These results are of scientific interest, but they provide little hope for the use of UV-excited fluorescence as a technique to perform safe standoff detection of adsorbed explosive particulates under real-world conditions with a useful degree of reliability.

  17. Capacitive sensing with a fluorescent lamp

    E-Print Network [OSTI]

    Cooley, John Jacob

    2007-01-01

    This work presents a modified fluorescent lamp that can be used as a capacitive sensing system. The lamp sensor measures changes in the electric fields emitted from the fluorescent bulbs in order to deduce the presence and ...

  18. Covered Product Category: Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  19. Covered Product Category: Fluorescent Ballasts

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and federal efficiency requirements across a variety of product categories, including fluorescent ballasts, which is a FEMP designated product category. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  20. Chemistry & Biology Conversion of Red Fluorescent Protein

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    resonance energy transfer applications. INTRODUCTION Green fluorescent protein (GFP) from Aequoria victoria for multicolor and lifetime imaging, as well as the outstanding donor for green fluorescent proteins in Fo¨ rster green fluorescent probes, such as TagGFP or EGFP (Shaner et al., 2005). The improvement

  1. Interconnection Standards

    Broader source: Energy.gov [DOE]

    West Virginia's interconnection standards include two levels of review. The qualifications and application fees for each level are as follows:...

  2. Fluorescence based chemical sensors for corrosion detection

    SciTech Connect (OSTI)

    Johnson, R.E.; Agarwala, V.S.

    1997-12-01

    Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.

  3. Microfabricated ion frequency standard

    DOE Patents [OSTI]

    Schwindt, Peter (Albuquerque, NM); Biedermann, Grant (Albuquerque, NM); Blain, Matthew G. (Albuquerque, NM); Stick, Daniel L. (Albuquerque, NM); Serkland, Darwin K. (Albuquerque, NM); Olsson, III, Roy H. (Albuquerque, NM)

    2010-12-28

    A microfabricated ion frequency standard (i.e. an ion clock) is disclosed with a permanently-sealed vacuum package containing a source of ytterbium (Yb) ions and an octupole ion trap. The source of Yb ions is a micro-hotplate which generates Yb atoms which are then ionized by a ultraviolet light-emitting diode or a field-emission electron source. The octupole ion trap, which confines the Yb ions, is formed from suspended electrodes on a number of stacked-up substrates. A microwave source excites a ground-state transition frequency of the Yb ions, with a frequency-doubled vertical-external-cavity laser (VECSEL) then exciting the Yb ions up to an excited state to produce fluorescent light which is used to tune the microwave source to the ground-state transition frequency, with the microwave source providing a precise frequency output for the ion clock.

  4. # ###### SQL # ``standard'' ###### ### ######### ###### #########.

    E-Print Network [OSTI]

    Pitoura, Evaggelia

    ' & $ % # ###### SQL # ``standard'' ###### ### ######### ###### #########. ###### Sequel #### IBM ## ##### ### System R, #### SQL (Stuctured Query Language) SQL--89, SQL--92 H SQL #### ####### #######: ffl ############# (authentication) ffl ########### ffl ###### ########## ###### ####### 1 #12; ' & $ % # ###### SQL 1. ###### #### 2

  5. Three-dimensional fluorescence lifetime tomography

    SciTech Connect (OSTI)

    Godavarty, Anuradha; Sevick-Muraca, Eva M.; Eppstein, Margaret J. [327 Votey Building, Department of Computer Science, University of Vermont, Burlington, Vermont 05405 (United States); 1011 Richardson Building, Photon Migration Laboratories, Texas A and M University, College Station, Texas 77843 (United States); 327 Votey Building, Department of Computer Science, University of Vermont, Burlington, Vermont 05405 (United States)

    2005-04-01

    Near-infrared fluorescence tomography using molecularly targeted lifetime-sensitive, fluorescent contrast agents have applications for early-stage cancer diagnostics. Yet, although the measurement of fluorescent lifetime imaging microscopy (FLIM) is extensively used in microscopy and spectroscopy applications, demonstration of fluorescence lifetime tomography for medical imaging is limited to two-dimensional studies. Herein, the feasibility of three-dimensional fluorescence-lifetime tomography on clinically relevant phantom volumes is established, using (i) a gain-modulated intensified charge coupled device (CCD) and modulated laser diode imaging system, (ii) two fluorescent contrast agents, e.g., Indocyanine green and 3-3'-Diethylthiatricarbocyanine iodide differing in their fluorescence lifetime by 0.62 ns, and (iii) a two stage approximate extended Kalman filter reconstruction algorithm. Fluorescence measurements of phase and amplitude were acquired on the phantom surface under different target to background fluorescence absorption (70:1, 100:1) and fluorescence lifetime (1:1, 2.1:1) contrasts at target depths of 1.4-2 cm. The Bayesian tomography algorithm was employed to obtain three-dimensional images of lifetime and absorption owing to the fluorophores.

  6. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, Daniel L. (Albuquerque, NM); Tangyunyong, Paiboon (Albuquerque, NM)

    1998-01-01

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC.

  7. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, D.L.; Tangyunyong, P.

    1998-01-06

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC. 1 fig.

  8. Portable spotter for fluorescent contaminants on surfaces

    DOE Patents [OSTI]

    Schuresko, Daniel D. (Oak Ridge, TN)

    1980-01-01

    A portable fluorescence-based spotter for polynuclear aromatic hydrocarbon contamination on personnel and work area surfaces under ambient lighting conditions is provided. This instrument employs beam modulation and phase sensitive detection for discriminating between fluorescence from organic materials from reflected background light and inorganic fluorescent material. The device uses excitation and emission filters to provide differentiation between classes of aromatic organic compounds. Certain inorganic fluorescent materials, including heavy metal compounds, may also be distinguished from the organic compounds, despite both having similar optical properties.

  9. LED Replacements for Linear Fluorescent Lamps Webcast

    Broader source: Energy.gov [DOE]

    In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting...

  10. EOS standards

    SciTech Connect (OSTI)

    Greeff, Carl W

    2011-01-12

    An approach to creating accurate EOS for pressure standards is described. Applications to Cu, Au, and Ta are shown. Extension of the method to high compressions using DFT is illustrated. Comparisons with modern functionals show promise.

  11. Mutations that suppress the thermosensitivity of green fluorescent protein

    E-Print Network [OSTI]

    Haseloff, Jim

    Mutations that suppress the thermosensitivity of green fluorescent protein Kirby R. Siemering*, Ralph Golbik, Richard Sever* and Jim Haseloff* Background: The green fluorescent protein (GFP temperatures. Background The green fluorescent protein (GFP) from the bio- luminescent jellyfish Aequorea

  12. Fiber optical assembly for fluorescence spectrometry

    DOE Patents [OSTI]

    Carpenter, II, Robert W. (Pagosa Springs, CO); Rubenstein, Richard (Staten Island, NY); Piltch, Martin (Los Alamos, NM); Gray, Perry (Los Alamos, NM)

    2010-12-07

    A system for analyzing a sample for the presence of an analyte in a sample. The system includes a sample holder for containing the sample; an excitation source, such as a laser, and at least one linear array radially disposed about the sample holder. Radiation from the excitation source is directed to the sample, and the radiation induces fluorescent light in the sample. Each linear array includes a plurality of fused silica optical fibers that receive the fluorescent light and transmits a fluorescent light signal from the first end to an optical end port of the linear array. An end port assembly having a photo-detector is optically coupled to the optical end port. The photo-detector detects the fluorescent light signal and converts the fluorescent light signal into an electrical signal.

  13. Applications of Nuclear Resonance Fluorescence

    SciTech Connect (OSTI)

    Warren, Glen A.; Detwiler, Rebecca S.; Peplowski, Patrick N.

    2010-11-11

    Nuclear Resonance Fluorescence (NRF) has the potential of addressing a wide variety of applications, which require isotopic and/or elemental information about a sample. We have investigated a variety of non-proliferation applications that may be addressed by NRF. From these applications, we have selected two, measuring uranium enrichment in UF6 cylinders and material verification in dismantlement, to investigate in more detail. Analytical models have been developed to evaluate these applications, and test measurements have been conducted to validate those models. We found that it is unlikely with current technology to address the requirements for UF6 cylinder enrichment measurements. In contrast, NRF is a very promising approach for material verification for dismantlement.

  14. Frequently Asked Questions Information on Compact Fluorescent Light Bulbs (CFLs) and Mercury

    E-Print Network [OSTI]

    Jia, Songtao

    home's electric bill. ENERGY STAR qualified CFLs use up to 75 percent less energy than incandescent, in one year it would save enough energy to light more than 3 million homes and prevent greenhouse gas, accessible change every American can make right now to reduce energy use at home and prevent greenhouse gas

  15. Using Nuclear Resonance Fluorescence for Nondestructive Isotopic Analysis

    E-Print Network [OSTI]

    Ludewigt, Bernhard A.

    2010-01-01

    7, (1959) pp. 54. [12] B.J. Quiter, ``Nuclear ResonanceFluorescence for Nuclear Materials Assay,'' University ofclandestine material with nuclear resonance fluorescence,"

  16. Glucose sensing molecules having selected fluorescent properties

    DOE Patents [OSTI]

    Satcher, Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-27

    An analyte sensing fluorescent molecule that employs intramolecular electron transfer is designed to exhibit selected fluorescent properties in the presence of analytes such as saccharides. The selected fluorescent properties include excitation wavelength, emission wavelength, fluorescence lifetime, quantum yield, photostability, solubility, and temperature or pH sensitivity. The compound comprises an aryl or a substituted phenyl boronic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. The fluorophore and switch component are selected such that the value of the free energy for electron transfer is less than about 3.0 kcal mol.sup.-1. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  17. Functional Area Qualification Standards | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12, 2015ExecutiveFluorescentDanKathyEnergy QualificationStandards Functional

  18. Healthcare Energy: Spotlight on Lighting and Other Electric Loads...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lighting and Other Electric Loads Healthcare Energy: Spotlight on Lighting and Other Electric Loads Compact fluorescent, light-emitting diode, and energy-saving incandescent light...

  19. Lesson11.doc

    E-Print Network [OSTI]

    B Compact fluorescent lightbulbs (CFL) can be used to most places that conventional incandescent bulbs are, but use a fraction of the electricity. This table lists ...

  20. Peering into a Quantum Well | U.S. DOE Office of Science (SC...

    Office of Science (SC) Website

    have unearthed droop's elusive cause, illuminating a key barrier to this potentially energy-saving alternative to conventional incandescent and fluorescent lighting. At issue,...

  1. Types of Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    selection. Types of lighting include: Fluorescent Incandescent Outdoor solar Light-emitting diode (LED) Also learn how energy-efficient lightbulbs compare to traditional...

  2. Understanding Drooping Light Emitting Diodes CEEM | U.S. DOE...

    Office of Science (SC) Website

    Impact Understanding "droop" may result in cheaper, more efficient LEDs; LEDs are more energy efficient, smaller, and longer-lived than incandescent lamps or fluorescent...

  3. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

  4. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, Edward S. (Ames, IA); Taylor, John A. (Nevada, IA)

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  5. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, Edward S. (Ames, IA); Taylor, John A. (Nevada, IA)

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  6. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

  7. Fluorescent Lamp Ballasts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12, 2015ExecutiveFluorescent Lamp Ballasts Fluorescent Lamp Ballasts The

  8. Laser excited confocal microscope fluorescence scanner and method

    DOE Patents [OSTI]

    Mathies, R.A.; Peck, K.

    1992-02-25

    A fluorescent scanner is designed for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier. The scanner includes a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from the volume to provide a display of the separated sample. 8 figs.

  9. Energy Standard

    Gasoline and Diesel Fuel Update (EIA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustments (Billion Cubic Feet)DecadeYear Jan Feb Mar Apr May Jun2003 Detailed Tablesof1998 and195 0Standard

  10. Analysis of Minimum Efficiency Performance Standards for Residential General Service Lighting in Chile

    SciTech Connect (OSTI)

    Letschert, Virginie E.; McNeil, Michael A.; Leiva Ibanez, Francisco Humberto; Ruiz, Ana Maria; Pavon, Mariana; Hall, Stephen

    2011-06-01

    Minimum Efficiency Performance Standards (MEPS) have been chosen as part of Chile's national energy efficiency action plan. As a first MEPS, the Ministry of Energy has decided to focus on a regulation for lighting that would ban the sale of inefficient bulbs, effectively phasing out the use of incandescent lamps. Following major economies such as the US (EISA, 2007) , the EU (Ecodesign, 2009) and Australia (AS/NZS, 2008) who planned a phase out based on minimum efficacy requirements, the Ministry of Energy has undertaken the impact analysis of a MEPS on the residential lighting sector. Fundacion Chile (FC) and Lawrence Berkeley National Laboratory (LBNL) collaborated with the Ministry of Energy and the National Energy Efficiency Program (Programa Pais de Eficiencia Energetica, or PPEE) in order to produce a techno-economic analysis of this future policy measure. LBNL has developed for CLASP (CLASP, 2007) a spreadsheet tool called the Policy Analysis Modeling System (PAMS) that allows for evaluation of costs and benefits at the consumer level but also a wide range of impacts at the national level, such as energy savings, net present value of savings, greenhouse gas (CO2) emission reductions and avoided capacity generation due to a specific policy. Because historically Chile has followed European schemes in energy efficiency programs (test procedures, labelling program definitions), we take the Ecodesign commission regulation No 244/2009 as a starting point when defining our phase out program, which means a tiered phase out based on minimum efficacy per lumen category. The following data were collected in order to perform the techno-economic analysis: (1) Retail prices, efficiency and wattage category in the current market, (2) Usage data (hours of lamp use per day), and (3) Stock data, penetration of efficient lamps in the market. Using these data, PAMS calculates the costs and benefits of efficiency standards from two distinct but related perspectives: (1) The Life-Cycle Cost (LCC) calculation examines costs and benefits from the perspective of the individual household; and (2) The National Perspective projects the total national costs and benefits including both financial benefits, and energy savings and environmental benefits. The national perspective calculations are called the National Energy Savings (NES) and the Net Present Value (NPV) calculations. PAMS also calculate total emission mitigation and avoided generation capacity. This paper describes the data and methodology used in PAMS and presents the results of the proposed phase out of incandescent bulbs in Chile.

  11. Air fluorescence relevant for cosmic-ray detection--Summary of the 5th fluorescence workshop, El Escorial 2007

    E-Print Network [OSTI]

    Hörandel, Jörg R.

    Air fluorescence relevant for cosmic-ray detection--Summary of the 5th fluorescence workshop, El August 2008 Keywords: Fluorescence yield Air showers a b s t r a c t High-energy cosmic rays with energies exceeding 1017 eV are frequently observed by measurements of the fluorescence light induced by air

  12. Ultratrace analysis of transuranic actinides by laser-induced fluorescence

    DOE Patents [OSTI]

    Miller, S.M.

    1983-10-31

    Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

  13. Replacing Incandescent Lightbulbs and Ballasts | Department of...

    Energy Savers [EERE]

    (R)or parabolic reflector (PAR) CFLs for flood and spotlighting. Some CFL fixtures have built-in electronic ballasts and polished metal reflectors. When used in recessed...

  14. Energy-efficient incandescent lamp: Final report

    E-Print Network [OSTI]

    Verderber, R.

    2010-01-01

    more energy than the best " Energy Ef- ficient Incandescentbest available conventional glass envelopes the greatest energyenvelope of best optical accuracy. The energy saving could

  15. Energy-efficient incandescent lamp: Final report

    E-Print Network [OSTI]

    Verderber, R.

    2010-01-01

    have doubled luminous efficacy, corresponding to 50% energyluminous than the clear lamp. The value of visible wattage 1n the energyluminous efficacy without l i f e reduction. Such efficacy Increase corresponds to an energy

  16. Incandescent Lighting Basics | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLAN FOR THE SITE-218in a V2O5 Battery Electrode

  17. Energy-Saving Incandescents | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submitKansasCommunities EnergyU.S. DOEEnergy StorageTricks Lead3Image icon

  18. Energy-Saving Incandescents | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submitKansasCommunities EnergyU.S. DOEEnergy StorageTricks Lead3Image iconFile

  19. A Study of the Fluorescence Response of Tetraphenyl-butadiene

    E-Print Network [OSTI]

    Jerry, R; Bugel, L; Conrad, J M

    2010-01-01

    Tetraphenyl-butadiene (TPB) is a widely used fluorescent wavelength-shifter. A common application is in liquid-argon-based particle detectors, where scintillation light is produced in the UV at 128 nm. In liquid argon experiments, TPB is often employed to shift the scintillation light to the visible range in order to allow detection via standard photomultiplier tubes. This paper presents studies on the stability of TPB with time under exposure to light. We also examine batch-to-batch variations. We compare scintillation-grade TPB to 99% pure TPB response. In the 99% pure samples, we report a yellowing effect, and full degradation of the TPB emission-peak, upon extended exposure to light.

  20. December 2006 Standards Forum and Standards Actions

    Energy Savers [EERE]

    -9 Cancellations in Progress - 0 Inside This Issue Visit the Technical Standards Program Web Site at http:tis.eh.doe.govtechstds December 2006 The Standards Forum and Standards...

  1. Magnetic fluorescent ballasts: Market data, market imperfections, and policy success

    SciTech Connect (OSTI)

    Koomey, J.G.; Sanstad, A.H.; Shown, L.J.

    1995-12-01

    Many economists have strongly questioned engineering-economic studies aimed at demonstrating anomalously slow diffusion of energy-efficient technology and the benefits of regulations to promote such technology. One argument against such studies is that standard techniques of engineering-economics are either inappropriate for or are routinely misapplied in assessing the performance of the market for energy efficiency. This paper presents engineering-economic evidence on the diffusion of energy efficiency improvements that takes account of such critiques. The authors examine the engineering and economic characteristics of standard and energy-efficient magnetic ballasts for fluorescent lighting. Efficient magnetic ballasts represented an excellent investment for 99% of the commercial building floor stock, and a moderately good investment for 0.7% of the commercial floor stock. Still, these ballasts were only being adopted in the 1980s at a rate commensurate with the enactment of appliance efficiency standards in various states. In this case, there is solid empirical evidence for skepticism about the effectiveness of the market mechanism in promoting cost-effective energy efficiency improvements as well as evidence of the benefits of regulation to counteract this shortcoming.

  2. Ultrabright fluorescent OLEDS using triplet sinks

    DOE Patents [OSTI]

    Zhang, Yifan; Forrest, Stephen R; Thompson, Mark

    2013-06-04

    A first device is provided. The first device further comprises an organic light emitting device. The organic light emitting device further comprises an anode, a cathode, and an emissive layer disposed between the anode and the cathode. The emissive layer further comprises an organic host compound, an organic emitting compound capable of fluorescent emission at room temperature, and an organic dopant compound. The triplet energy of the dopant compound is lower than the triplet energy of the host compound. The dopant compound does not strongly absorb the fluorescent emission of the emitting compound.

  3. Questioning Copyright in Standards

    E-Print Network [OSTI]

    Samuelson, Pam

    2006-01-01

    of privately drafted industry standards). Antitrust problems40, at 308. Thus, industry standards such as the AMA and ADAprotection for industry standards is the claim SSOs make

  4. Performance of T12 and T8 Fluorescent Lamps and Troffers and LED Linear Replacement Lamps CALiPER Benchmark Report

    SciTech Connect (OSTI)

    Myer, Michael; Paget, Maria L.; Lingard, Robert D.

    2009-01-16

    The Department of Energy (DOE) Commercially Available LED Product Evaluation and Reporting (CALiPER) Program was established in 2006 to investigate the performance of light-emitting diode (LED) based luminaires and replacement lamps. To help users better compare LED products with conventional lighting technologies, CALiPER has also performed benchmark research and testing of traditional (i.e., non-LED) lamps and fixtures. This benchmark report addresses standard 4-foot fluorescent lamps (i.e., T12 and T8) and the 2-foot by 4-foot recessed troffers in which they are commonly used. This report also examines available LED replacements for T12 and T8 fluorescent lamps, and their application in fluorescent troffers. The construction and operation of linear fluorescent lamps and troffers are discussed, as well as fluorescent lamp and fixture performance, based on manufacturer data and CALiPER benchmark testing. In addition, the report describes LED replacements for linear fluorescent lamps, and compares their bare lamp and in situ performance with fluorescent benchmarks on a range of standard lighting measures, including power usage, light output and distribution, efficacy, correlated color temperature, and the color rendering index. Potential performance and application issues indicated by CALiPER testing results are also examined.

  5. Deep tissue fluorescence imaging and in vivo biological applications

    E-Print Network [OSTI]

    2012-01-01

    17(11) Crosignani et al. : Deep tissue fluorescence imaging16. D. Kobat et al. , “Deep tissue multiphoton microscopyV. Crosignani et al. , “ In vivo deep tissue fluorescence

  6. Imaging chromophores with undetectable fluorescence by stimulated emission microscopy

    E-Print Network [OSTI]

    Xie, Xiaoliang Sunney

    LETTERS Imaging chromophores with undetectable fluorescence by stimulated emission microscopy Wei, that is, spontaneous emission, is generally more sensitive than absorption measurement, and is widely used undetectable fluorescence because the spontaneous emission is dominated by theirfastnon-radiative decay3

  7. Probing the denatured state ensemble with fluorescence 

    E-Print Network [OSTI]

    Alston, Roy Willis

    2004-09-30

    of these five proteins were studied and compared to the fluorescent properties of eight model compounds: N-acetyl-tryptophan-amide (NATA), N-acetyl-Ala-Trp-Ala-amide (AWA), N-acetyl-Ala-Ala-Trp-Ala-Ala-amide (AAWAA), and five pentapeptides based on the sequence...

  8. Chlorophyll Fluorescence Monitoring at Selected LTER

    E-Print Network [OSTI]

    Boyce, Richard L.

    no energy can go to photosynthesis · Thermal dissipation processes are usually minimal in the dark · So most of plant stress on photosynthesis in the field Fate of Light absorbed by Chlorophyll · Energy channeled to reaction center of PSII, used for photosynthesis ­ P · Thermal dissipation ­ D · Fluorescent emission ­ F

  9. Chemistry & Biology Red Fluorescent Protein with Reversibly

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    to the photoswitchable absor- bance, rsTagRFP can be used as an acceptor for a photochromic Fo¨ rster resonance energy-OFF photoswitching of the rsTagRFP acceptor. INTRODUCTION Green fluorescent protein (GFP) from Aequorea victoriaTFP0.7 (Henderson et al., 2007), green Dronpa (Ando et al., 2004) and its derivatives (Ando et al

  10. Atomic and Molecular Fluorescence Excited by Photodissociation

    E-Print Network [OSTI]

    electron impact processes are compared with photodissociation. A bibliography of photo dissociation the strength a n d shape of fluorescence lines that arise from excited fragments produced in molecular photo more critical. T h e D o p p l e r width of the emission is important, however, since a larger width

  11. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

    1992-05-12

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

  12. Imaging properties of supercritical angle fluorescence optics

    E-Print Network [OSTI]

    Enderlein, Jörg

    Imaging properties of supercritical angle fluorescence optics J¨org Enderlein,1,4, Ingo Gregor,1.ruckstuhl@pci.uzh.ch 4http://www.joerg-enderlein.de enderlein@physik3.gwdg.de Abstract: In recent years, new optical the detection volume within one wavelength to an interface. For conventional optical systems with high numerical

  13. Bayesian fluorescence in situ hybridisation signal classification

    E-Print Network [OSTI]

    Lerner, Boaz

    hybridisation (FISH) signals for the detection of genetic abnormalities. Based on well-discriminating features hybridisation (FISH); Gaussian mixture model; Naive Bayesian classifier; Signal classification Artificial.artmed.2003.11.005 #12;1. Introduction Fluorescence in situ hybridisation (FISH) allows selective staining

  14. Oil Classification with Fluorescence Spectroscopy Engineering Physics

    E-Print Network [OSTI]

    Oldenburg, Carl von Ossietzky Universität

    detected by these channels. The investigation used three methods to examine crude oil, heavy oil, sludge1 Oil Classification with Fluorescence Spectroscopy Engineering Physics Master of Engineering and classification of oil spills on water surfaces. It is an overview of the laser remote sensor technique

  15. Purchasing Energy-Efficient Suspended Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    The Federal Energy Management Program (FEMP) provides acquisition guidance for suspended fluorescent luminaires, a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR-qualified or FEMP-designated products in all product categories covered by these programs and in any acquisition actions that are not specifically exempted by law.

  16. Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements

    E-Print Network [OSTI]

    Liyanage, Mangala Roshan

    2009-01-01

    Calmodulin (CaM) is a calcium signaling protein that activates over hundred of targets including PMCA. This dissertation mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions...

  17. Apparatus for eliminating background interference in fluorescence measurements

    DOE Patents [OSTI]

    Martin, J.C.; Jett, J.H.

    1986-03-04

    The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.

  18. Text-Alternative Version: LED Replacements for Linear Fluorescent Lamps

    Broader source: Energy.gov [DOE]

    Below is the text-alternative version of the "LED Replacements for Linear Fluorescent Lamps" webcast, held June 20, 2011.

  19. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are aminomore »acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.« less

  20. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    SciTech Connect (OSTI)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

  1. Imaging green fluorescent protein in transgenic plants. Jim Haseloff

    E-Print Network [OSTI]

    Haseloff, Jim

    light, and an accessory green fluorescent protein (GFP), which accepts energy from aequorin and reImaging green fluorescent protein in transgenic plants. Jim Haseloff MRC Laboratory of Molecular of seedlings. The green fluorescent protein (GFP) from the cnidarian jellyfish Aequorea victoria shares none

  2. 10081 How snakes slither 10086 Biodegradable fluorescent polymers

    E-Print Network [OSTI]

    Yang, Jian

    10081 How snakes slither 10086 Biodegradable fluorescent polymers 10097 Studying forces within, and Michael J. Shelley (see pages 10081­10085) CHEMISTRY Biodegradable fluorescent polymers Although fluorescent polymers that can be crosslinked into membranes. The polymers may serve as scaffolds in tissue

  3. Lanthanide and actinide doped glasses as reference standards for dye doped systems

    SciTech Connect (OSTI)

    Pope, E.J.A.; Hentschel, A.

    1996-12-31

    Organic dye molecules are well known to be subject to chemical and optical bleaching damage, temperature instability, and other forms of optical degradation. Currently recognized methods of referencing rely upon fluorescent salt solutions, such as quinine sulfate. In this paper, optically-active lanthanide and actinide doped gel-glasses are compared as reference standards for dye doped polymers. Samples are subjected to continuous illumination by 254 nm UV radiation. While dye-doped polymers exhibited approximately 65 percent decline in fluorescence intensity after 96 hours of irradiation, glass samples and glass powder in resin showed no decline in fluorescence intensities.

  4. Invisible-fluorescent identification tags for materials

    SciTech Connect (OSTI)

    Lewis, Linda A.; Allgood, Glenn O.; Smithwick, III, Robert W.

    2013-03-26

    A taggant composition including a taggant material that is invisible in light of the visible spectrum and fluoresces under a non-visible excitation energy, a binder, and a solvent in which the taggant material and the binder are dissolved. The taggant composition can be printed or otherwise applied to a material such as fabric to provide a detectable and identifiable indicium. A method and apparatus for detecting and decoding the taggant indicium are also provided.

  5. Design and evaluation of a device for fast multispectral time-resolved fluorescence spectroscopy and imaging

    SciTech Connect (OSTI)

    Yankelevich, Diego R.; Department of Biomedical Engineering, University of California, 451 Health Sciences Drive, Davis, California 95616 ; Ma, Dinglong; Liu, Jing; Sun, Yang; Sun, Yinghua; Bec, Julien; Marcu, Laura; Elson, Daniel S.

    2014-03-15

    The application of time-resolved fluorescence spectroscopy (TRFS) to in vivo tissue diagnosis requires a method for fast acquisition of fluorescence decay profiles in multiple spectral bands. This study focusses on development of a clinically compatible fiber-optic based multispectral TRFS (ms-TRFS) system together with validation of its accuracy and precision for fluorescence lifetime measurements. It also presents the expansion of this technique into an imaging spectroscopy method. A tandem array of dichroic beamsplitters and filters was used to record TRFS decay profiles at four distinct spectral bands where biological tissue typically presents fluorescence emission maxima, namely, 390, 452, 542, and 629 nm. Each emission channel was temporally separated by using transmission delays through 200 ?m diameter multimode optical fibers of 1, 10, 19, and 28 m lengths. A Laguerre-expansion deconvolution algorithm was used to compensate for modal dispersion inherent to large diameter optical fibers and the finite bandwidth of detectors and digitizers. The system was found to be highly efficient and fast requiring a few nano-Joule of laser pulse energy and <1 ms per point measurement, respectively, for the detection of tissue autofluorescent components. Organic and biological chromophores with lifetimes that spanned a 0.8–7 ns range were used for system validation, and the measured lifetimes from the organic fluorophores deviated by less than 10% from values reported in the literature. Multi-spectral lifetime images of organic dye solutions contained in glass capillary tubes were recorded by raster scanning the single fiber probe in a 2D plane to validate the system as an imaging tool. The lifetime measurement variability was measured indicating that the system provides reproducible results with a standard deviation smaller than 50 ps. The ms-TRFS is a compact apparatus that makes possible the fast, accurate, and precise multispectral time-resolved fluorescence lifetime measurements of low quantum efficiency sub-nanosecond fluorophores.

  6. Communication Standards and Recommendations

    E-Print Network [OSTI]

    Communication Standards and Recommendations Introduction & Purpose 3 Standards & Recommendations Communication 4 Training 10 Evaluation 11 PMO Workgroup Participation 12 Staffing 12 Communications-related Tracking Grantee Portal Standards and Recommendations 13

  7. Environmental Standardization for Sustainability

    E-Print Network [OSTI]

    Bagby, John

    Environmental Standardization for Sustainability by Professor John W. Bagby College of Information that environmental controls are expressed as environmental standards, a traditional driver of investment in pollution control. Environmental standards spur investment in green technologies that promise to stimulate

  8. Energy Efficiency Product Standards

    Broader source: Energy.gov [DOE]

    New Jersey Energy Efficiency Product Standards, enacted in 2005, include minimum standards for eight products, which were preempted by the federal Energy Policy Act of 2005. Future standards, if...

  9. Fluorescence Rise Time Measurements for High Temperature Fluorescence-Based Thermometry

    SciTech Connect (OSTI)

    Allison, S.W.

    2005-03-24

    Certain ceramic-like phosphor materials exhibit bright fluorescence with a pronounced temperature dependence over a range which spans the cryogenic to 1700 C, depending on the specific phosphor. To measure temperature, a surface, for instance a turbine blade, is coated with the material. An optical system, sometimes including optical fibers, conveys stimulating light and collects the emission for analysis. Either emission intensity or decay time may indicate temperature. Previously fielded tests have involved surfaces such as blades, vanes, pistons, in-take valves, sheets of galvanneal steel, etc. The fluorescent coatings may be applied to small parts via sputtering methods or to large areas by mixture with inorganic binders. Presented here are results characterizing fluorescence rise times as a means of determining temperature from ambient to 700 C for Y{sub 2}O{sub 3}:Eu.

  10. Energy Efficiency Resource Standard

    Office of Energy Efficiency and Renewable Energy (EERE)

    Washington voters passed Initiative 937 in 2006, creating a renewable energy standard and an energy efficiency resource standard for the state's electric utilities. Initiative 937, enacted as th...

  11. Technical Standards,DOE Standards and Corresponding Directives...

    Broader source: Energy.gov (indexed) [DOE]

    Directives Crosswalk DOE Standards and Corresponding Directives Crosswalk table Technical Standards,DOE Standards and Corresponding Directives Crosswalk More Documents &...

  12. Technical Standards Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1999-11-19

    The Technical Standards Program (TSP) promotes the use of voluntary consensus standards by the Department of Energy (DOE), provides DOE with the means to develop needed technical standards, and manages overall technical standards information, activities, issues, and interactions. Cancels DOE O 1300.2A. Canceled by DOE O 252.1A

  13. Technical Standards Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-02-23

    The Order promotes DOE's use of Voluntary Consensus Standards (VCS) as the primary method for application of technical standards and establishes and manages the DOE Technical Standards Program (TSP) including technical standards development, information, activities, issues, and interactions. Admin Chg 1 dated 3-12-13.

  14. Solid state laser media driven by remote nuclear powered fluorescence

    DOE Patents [OSTI]

    Prelas, Mark A. (Columbia, MO)

    1992-01-01

    An apparatus is provided for driving a solid state laser by a nuclear powered fluorescence source which is located remote from the fluorescence source. A nuclear reaction produced in a reaction chamber generates fluorescence or photons. The photons are collected from the chamber into a waveguide, such as a fiber optic waveguide. The waveguide transports the photons to the remote laser for exciting the laser.

  15. Nuclear Resonance Fluorescence for Nuclear Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian Joseph

    2010-01-01

    to Journal of Nuclear Technology. [46] C.J. Hagmann and J.Library for Nuclear Science and Technology,” Nuclear Dataof Standards and Technology daughter nuclear data processing

  16. Fluorescent dyes for probing cellular functions associated with...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    fluorescence dyes suitable for visualization of specific cellular functions associated with electron beam damage and carry out electron microscopy imaging of labelled cells....

  17. Time-resolved fluorescence decay measurements for flowing particles

    DOE Patents [OSTI]

    Deka, C.; Steinkamp, J.A.

    1999-06-01

    Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.

  18. Fluorescent protein marker lines in maize: generation and applications

    E-Print Network [OSTI]

    Jackson, David

    : fluorescent protein, FRET, maize, pOp, LhG4 Introduction Maize, one of the most important crops worldwide, has

  19. Fluorescent Pigments for High-Performance Cool Roofing and Facades...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to develop a new class of dark-colored pigments for cool metal roof and faade coatings that incorporate near-infrared fluorescence and reflectance to improve energy...

  20. Organimetallic Fluorescent Complex Polymers For Light Emitting Applications

    DOE Patents [OSTI]

    Shi, Song Q. (Phoenix, AZ); So, Franky (Tempe, AZ)

    1997-10-28

    A fluorescent complex polymer with fluorescent organometallic complexes connected by organic chain spacers is utilized in the fabrication of light emitting devices on a substantially transparent planar substrate by depositing a first conductive layer having p-type conductivity on the planar surface of the substrate, depositing a layer of a hole transporting and electron blocking material on the first conductive layer, depositing a layer of the fluorescent complex polymer on the layer of hole transporting and electron blocking material as an electron transporting emissive layer and depositing a second conductive layer having n-type conductivity on the layer of fluorescent complex polymer.

  1. Quantitative planar laser-induced fluorescence imaging of multi...

    Office of Scientific and Technical Information (OSTI)

    Article: Quantitative planar laser-induced fluorescence imaging of multi-component fuelair mixing in a firing gasoline-direct-injection engine: Effects of residual exhaust...

  2. H_2 Absorption and Fluorescence for Gamma Ray Bursts in Molecular Clouds

    E-Print Network [OSTI]

    B. T. Draine

    1999-07-16

    If a gamma ray burst with strong UV emission occurs in a molecular cloud, there will be observable consequences resulting from excitation of the surrounding H2. The UV pulse from the GRB will pump H2 into vibrationally-excited levels which produce strong absorption at wavelengths < 1650 A. As a result, both the prompt flash and later afterglow will exhibit strong absorption shortward of 1650 A, with specific spectroscopic features. Such a cutoff in the emission from GRB 980329 may already have been observed by Fruchter et al.; if so, GRB 980329 was at redshift 3.0 < z < 4.4 . BVRI photometry of GRB 990510 could also be explained by H2 absorption if GRB 990510 is at redshift 1.6 < z < 2.3. The fluorescence accompanying the UV pumping of the H2 will result in UV emission from the GRB which can extend over days or months, depending on parameters of the ambient medium and beaming of the GRB flash. The 7.5-13.6 eV fluorescent luminosity is \\sim 10^{41.7} erg/s for standard estimates of the parameters of the GRB and the ambient medium. Spectroscopy can distinguish this fluorescent emission from other possible sources of transient optical emission, such as a supernova.

  3. Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    CHAPTER 17 Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry Kiryl D. Piatkevich. Simultaneous Detection of Multiple FPs V. Fluorescent Timers VI. FRET-Based Genetically Encoded Biosensors A. Conventional FRET Pairs B. Novel Advanced FRET Pairs C. FRET Biosensors in Multicolor Flow Cytometry D

  4. REVIEW ARTICLE Planar laser induced fluorescence in aqueous flows

    E-Print Network [OSTI]

    Crimaldi, John P.

    REVIEW ARTICLE Planar laser induced fluorescence in aqueous flows J. P. Crimaldi Received: 14 for measuring scalar concentra- tions in fluid flows. A fluorescent dye is used as a scalar proxy, and local Osbourne Reynolds performed his famous experiments on laminar and turbulent flow in round pipes (Reynolds

  5. Fluorescence Probe Studies of Gelatin-Sodium Dodecyl Sulfate Interactions

    E-Print Network [OSTI]

    Bales, Barney

    Fluorescence Probe Studies of Gelatin-Sodium Dodecyl Sulfate Interactions P. C. Griffiths* and J. A dodecyl sulfate (SDS) micelles bound to gelatin have been studied by fluorescence using 8-anilino-1-naphththalene sulfonic acid (ANS) as probe. Like gelatin, ANS binds in the region of the micelle occupied

  6. Teal fluorescent proteins: Characterization of a reversibly photoswitchable variant

    E-Print Network [OSTI]

    Campbell, Robert E.

    . Cyan FPs (CFPs), widely used in multicolor imaging or as a fluorescence resonance energy transfer (FRET green fluorescent protein (GFP) in 1961 when he was isolating aequorin, a calcium cell imaging due to drawbacks such as dual excitation peaks and poor folding efficiency. Fortunately

  7. Improved ``Optical Highlighter'' Probes Derived from Discosoma Red Fluorescent Protein

    E-Print Network [OSTI]

    Marchant, Jonathan

    , Minneapolis, Minnesota ABSTRACT The tetrameric red fluorescent protein, DsRed, undergoes a rapid red to green-emitting species of DsRed and an enhancement of emission from the ``immature'' green species, likely caused by dequenching of fluorescence resonance energy transfer occurring within the protein tetramer. Here, we have

  8. TECHNICAL STANDARDS PROGRAM RESPONSIBILITIES

    Broader source: Energy.gov [DOE]

    PurposeThis procedure describes the responsibilities of persons who are charged with implementing the DOE Technical Standards Program. 

  9. TECHNICAL STANDARDS COMMENT RESOLUTION

    Broader source: Energy.gov [DOE]

    PurposeThis procedure provides guidance for resolving comments on DOE Technical Standards that are received during the coordination process. 

  10. Surface Water Quality Standards 

    E-Print Network [OSTI]

    Wythe, Kathy

    2007-01-01

    Standards Team. This advisory group, with representation from water asso- ciations, the agricultural industry, engineering firms, environmental organizations, consumer groups and government entities, is working with TCEQ staff to review and possibly...SURFACE WATER QUALITY STANDARDS AAs part of the ongoing program to manage Texaswater quality, the Texas Commission onEnvironmental Quality (TCEQ) is currently review- ing the Texas Surface Water Quality Standards, including the standards...

  11. Radioiodine detector based on laser induced fluorescence

    DOE Patents [OSTI]

    McDonald, Jimmie R. (Upper Marlboro, MD); Baronavski, Andrew P. (Alexandria, VA)

    1980-01-01

    The invention involves the measurement of the concentration of the radioisotope .sup.129 I.sub.2 in the presence of a gas. The invention uses a laser to excite a sample of the .sup.129 I.sub.2 in a sample gas chamber and a reference sample of a known concentration of .sup.129 I.sub.2 in a reference gas chamber. The .sup.129 I.sub.2 in the sample and reference gas chamber each gives off fluorescence emissions which are received by photomultipliers which provide signals to a detector. The detector uses a ratioing technique to determine the concentration of .sup.129 I.sub.2 in the sample gas chamber.

  12. Real time PCR measurement by fluorescence anisotropy

    E-Print Network [OSTI]

    Crane, Bryan Lee, 1976-

    2005-01-01

    Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression ...

  13. Interdomain Fluorescence Resonance Energy Transfer in SERCA Probed by Cyan-Fluorescent Protein Fused to the Actuator Domain

    E-Print Network [OSTI]

    Thomas, David D.

    ), the measured FRET efficiency between CFP (donor in A domain) and FITC (acceptor in N domain) was 0.34 ( 0Interdomain Fluorescence Resonance Energy Transfer in SERCA Probed by Cyan-Fluorescent Protein transport by the sarcoendoplasmic reticulum Ca-ATPase (SERCA) from rabbit fast- twitch skeletal muscle. X

  14. Air Fluorescence Relevant for Cosmic-Ray Detection - Summary of the 5th Fluorescence Workshop, El Escorial 2007

    E-Print Network [OSTI]

    Fernando Arqueros; Joerg R. Hoerandel; Bianca Keilhauer

    2008-07-24

    High-energy cosmic rays with energies exceeding $10^{17}$ eV are frequently observed by measurements of the fluorescence light induced by air showers. A major contribution to the systematic uncertainties of the absolute energy scale of such experiments is the insufficient knowledge of the fluorescence light yield of electrons in air. The aim of the 5th Fluorescence Workshop was to bring together experimental and theoretical expertise to discuss the latest progress on the investigations of the fluorescence light yield. The results of the workshop will be reviewed as well as the present status of knowledge in this field. Emphasis is given to the fluorescence light yield important for air shower observations and its dependence on atmospheric parameters, like pressure, temperature, and humidity. The effects of the latest results on the light observed from air showers will be discussed.

  15. Improved fluorescence-enhanced optical imaging and tomography by enhanced excitation light rejection 

    E-Print Network [OSTI]

    Hwang, Kil Dong

    2009-05-15

    Fluorescence enhanced optical imaging and tomography studies involve the detection of weak fluorescent signals emanating from nano- to picomolar concentrations of exogenous or endogenously produced fluorophore concurrent ...

  16. Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

    SciTech Connect (OSTI)

    Bernassau, A. L.; Al-Rawhani, M.; Beeley, J.; Cumming, D. R. S. [School of Engineering, University of Glasgow, Glasgow, G12 8LT (United Kingdom)] [School of Engineering, University of Glasgow, Glasgow, G12 8LT (United Kingdom)

    2013-12-09

    A compact hybrid system has been developed to position and detect fluorescent micro-particles by combining a Single Photon Avalanche Diode (SPAD) imager with an acoustic manipulator. The detector comprises a SPAD array, light-emitting diode (LED), lenses, and optical filters. The acoustic device is formed of multiple transducers surrounding an octagonal cavity. By stimulating pairs of transducers simultaneously, an acoustic landscape is created causing fluorescent micro-particles to agglomerate into lines. The fluorescent pattern is excited by a low power LED and detected by the SPAD imager. Our technique combines particle manipulation and visualization in a compact, low power, portable setup.

  17. Fluorescent lamp unit with magnetic field generating means

    DOE Patents [OSTI]

    Grossman, M.W.; George, W.A.

    1989-08-08

    A fluorescent lamp unit having a magnetic field generating means for improving the performance of the fluorescent lamp is disclosed. In a preferred embodiment the fluorescent lamp comprises four longitudinally extending leg portions disposed in substantially quadrangular columnar array and joined by three generally U-shaped portions disposed in different planes. In another embodiment of the invention the magnetic field generating means comprises a plurality of permanent magnets secured together to form a single columnar structure disposed within a centrally located region defined by the shape of lamp envelope. 4 figs.

  18. Energy Conservation Standards Activities

    Broader source: Energy.gov (indexed) [DOE]

    Congress August 2014 United States Department of Energy Washington, DC 20585 Energy Conservation Standards Activities Report to Congress | Page i Message from the Assistant...

  19. STANDARD REVIEW PLAN

    Office of Environmental Management (EM)

    Strengthening Line Management Oversight and Federal Monitoring of Nuclear Facilities Standard Review Plan Safety Design Strategy January 2015 OFFICE OF ENVIRONMENTAL MANAGEMENT...

  20. Renewable Energy Standard

    Broader source: Energy.gov [DOE]

    Note: H.B. 40, enacted in June 2015, created Vermont's Renewable Energy Standard and repeals the Sustainably Priced Energy Enterprise Development program's renewable energy goals. The Renewable...

  1. SSL Standards and Guidelines

    SciTech Connect (OSTI)

    None

    2012-04-01

    Solid-state lighting program technology fact sheet that reviews the key performance and safety standards applicable to SSL-based lighting products.

  2. The Standard Model

    ScienceCinema (OSTI)

    Lincoln, Don

    2014-08-12

    Fermilab scientist Don Lincoln describes the Standard Model of particle physics, covering both the particles that make up the subatomic realm and the forces that govern them.

  3. Standard Subject Classification System

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1979-08-14

    The order establishes the DOE Standard Subject Classification System for classifying documents and records by subject, including correspondence, directives, and forms.Cancels DOE O 0000.1.

  4. Technical Standards Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-02-23

    The order establishes the DOE Technical Standards Program. Admin Chg 1, dated 3-12-13 supersedes DOE O 252.1A.

  5. Law as a Network Standard

    E-Print Network [OSTI]

    Burk, DL

    2006-01-01

    benefits of uniform technical standards, and the problemsparallel to those in the analysis of technical standards.Uniform standards, whether technical or legal, give rise to

  6. Fourteen Points for Energy Conservation in Lighting 

    E-Print Network [OSTI]

    Zekowski, G.

    1979-01-01

    are the approximate lumen output of various lamp types: Incandescent - 17 lumens per watt; mer cury-vapor - 55 lumens per watt; fluorescent - 65 lumens per watt; metal hal ide - 95 lumens per watt; and hi pressure sodium - 120 lumens per watt. As an example, using... a low 4?/Kw, ten hours per day, you can save: $10 per year using 1-32 watt fluorescent rather than 2-60 watt incandescents. Or $33 per year by using 1-40 watt fluorescent rather than 4-60 watt incandescents. Or $33 per year using 1-175 watt mercury...

  7. Large core polymer optical backplanes for fluorescence detection

    E-Print Network [OSTI]

    Lee, Kevin Shao-Kwan

    2006-01-01

    Fluorescence based sensors are used for determining environmental parameters such as dissolved oxygen or pH in biological systems without disturbing a biological system's equilibrium. Recently, there has been a drive to ...

  8. THE HISTORY AND TECHNICAL EVOLUTION OF HIGH FREQUENCY FLUORESCENT LIGHTING

    E-Print Network [OSTI]

    Campbell, John H.

    2011-01-01

    Dec. 1953. "Hi-Volt-Cycle Lighting ~ Launched i n a Hi-Wide2, F e b r u a r y , 19 53. "Lighting Your Plant with Highg h Frequency Fluorescent Lighting John H. Campbell December

  9. Enlightening Medicago truncatula transformation and shading GFP fluorescence 

    E-Print Network [OSTI]

    Zhou, Xin

    2005-11-01

    cotyledons of most transgenic M. truncatula lines, silencing of the GUS expression from the phas promoter was observed in several lines, indicating the occurrence of novel epigenetic events. The diminution of GFP fluorescence in transgenic M. truncatula...

  10. Protein subcellular localization assays using split fluorescent proteins

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2009-09-08

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  11. Phase-sensitive fluorescent imaging with coherent reconstruction

    E-Print Network [OSTI]

    Field, Jeffrey J; Bartels, Randy A

    2015-01-01

    Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light emerging from a specimen, to extract a three-dimensional representation of the object from a single high-speed measurement. Until now, CI methods could not be applied to incoherent light, severely limiting their ability to image the most powerful biological probes available - fluorescent molecules - with sufficient speed and volume to observe important processes, such as neural processing in live specimens. We introduce a new imaging technique that transfers the spatial propagation phase of coherent illumination light to incoherent fluorescent light emission. The transfer of propagation phase allows CI techniques to be applied to fluorescent light imaging, and leads to large increases in imaging speed and depth of field. With this advance, biological imaging of fluorescent ...

  12. Dynamic fluorescence imaging with molecular agents for cancer detection 

    E-Print Network [OSTI]

    Kwon, Sun Kuk

    2009-05-15

    -wavelength fluorescence imaging was accomplished using a liquid crystal tunable filter (LCTF). Excitation light was used for reflectance images with a LCTF transmitting a shorter wavelength than the peak in the excitation light spectrum. Therefore, images can...

  13. Exploring the mechanome with optical tweezers and single molecule fluorescence

    E-Print Network [OSTI]

    Brau, Ricardo R. (Ricardo Rafael), 1979-

    2008-01-01

    The combination of optical tweezers and single molecule fluorescence into an instrument capable of making combined, coincident measurements adds an observable dimension that allows for the examination of the localized ...

  14. SHORT COMMUNICATION The Chlorophyll Fluorescence Ratio F735/F700

    E-Print Network [OSTI]

    Gitelson, Anatoly

    sylvatica L.), elm (Ulmus minor 1988; Dahn et al., 1992). In green leaves, about 90% of Miller), and wild of reabsorption of the Chl fluorescence was quan- titatively estimated (Dahn et al., 1992; Gunther et al

  15. FY08 Annual Report for Nuclear Resonance Fluorescence Imaging

    SciTech Connect (OSTI)

    Warren, Glen A.; Caggiano, Joseph A.

    2009-01-06

    FY08 annual report for project the "Nuclear Resonance Fluorescence Imaging" project. Reviews accomplishments of last 3 years, including U-235 signature search, comparison of different photon sources, and examination of NRF measurements using monochromatic photon source.

  16. Narrowband fluorescent nanodiamonds produced from chemical vapor deposition films

    E-Print Network [OSTI]

    Neu, Elke; Gross, Elke; Hepp, Christian; Steinmetz, David; Zscherpel, Elisabeth; Ghodbane, Slimane; Sternschulte, Hadwig; Steinmueller-Nethl, Doris; Liang, Yuejiang; Krueger, Anke; Becher, Christoph

    2011-01-01

    We report on the production of nanodiamonds (NDs) with 70-80 nm size via bead assisted sonic disintegration (BASD) of a polycrystalline chemical vapor deposition (CVD) film. The NDs display high crystalline quality as well as intense narrowband (7 nm) room temperature luminescence at 738 nm due to in situ incorporated silicon vacancy (SiV) centers. The fluorescence properties at room and cryogenic temperatures indicate that the NDs are, depending on preparation, applicable as single photon sources or as fluorescence labels.

  17. Narrowband fluorescent nanodiamonds produced from chemical vapor deposition films

    E-Print Network [OSTI]

    Elke Neu; Carsten Arend; Felix Guldner; Elke Gross; Christian Hepp; David Steinmetz; Elisabeth Zscherpel; Slimane Ghodbane; Hadwig Sternschulte; Doris Steinmueller-Nethl; Yuejiang Liang; Anke Krueger; Christoph Becher

    2011-05-24

    We report on the production of nanodiamonds (NDs) with 70-80 nm size via bead assisted sonic disintegration (BASD) of a polycrystalline chemical vapor deposition (CVD) film. The NDs display high crystalline quality as well as intense narrowband (7 nm) room temperature luminescence at 738 nm due to in situ incorporated silicon vacancy (SiV) centers. The fluorescence properties at room and cryogenic temperatures indicate that the NDs are, depending on preparation, applicable as single photon sources or as fluorescence labels.

  18. Fluorescent Labeling Reagents Optimized for Capillary Electrophoretic Separations 

    E-Print Network [OSTI]

    Estrada, Roy Tonacao, III

    2012-02-14

    molecules fluoresce, background noise from the separation matrix and other extraneous compounds is usually a problem. Moreover, UV lasers tend to be more expensive than those used in the visible region [8]. To go around the background noise problem..., excitation can be done in the visible or higher wavelengths. However, only a very small number of proteins have native fluorescence above the UV region. To effectively use LIF as a detection method, proteins have to be labeled with fluorophores that can...

  19. Method and apparatus for detection of fluorescently labeled materials

    DOE Patents [OSTI]

    Stern, David (Mt. View, CA); Fiekowsky, Peter (Los Altos, CA)

    1997-01-01

    Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

  20. Method and apparatus for detection of fluorescently labeled materials

    DOE Patents [OSTI]

    Stern, David; Fiekowsky, Peter

    2004-05-25

    Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

  1. Measurement of Flow Velocity and Inference of Liquid Viscosity in a Microfluidic Channel by Fluorescence Photobleaching

    E-Print Network [OSTI]

    and determine their contributions to fluorescence recovery in an infused liquid medium.12 The fundamental

  2. Standard version Advanced version

    E-Print Network [OSTI]

    Hall, Julian

    Standard version Advanced version Xpress case study: Margaret Oil 26 June 2014 NATCOR Case study #12;Standard version Advanced version Margaret Oil - basic (1) Margaret Oil produces three products: gasoline, jet fuel, and heating oil. The average octane levels must be: Gasoline Jet fuel Heating oil

  3. LucY: A versatile new fluorescent reporter protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Auldridge, Michele E.; Cao, Hongnan; Sen, Saurabh; Franz, Laura P.; Bingman, Craig A.; Yennamalli, Ragothaman M.; Phillips, Jr., George N.; Mead, David; Steinmetz, Eric J.; Michnick, Stephen W.

    2015-04-23

    We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276nm, 377nm, and 460nm and a single emission peak at 530nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrastmore »to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.« less

  4. Rigidifying Fluorescent Linkers by Metal-Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement

    SciTech Connect (OSTI)

    Wei, ZW; Gu, ZY; Arvapally, RK; Chen, YP; McDougald, RN; Ivy, JF; Yakovenko, AA; Feng, DW; Omary, MA; Zhou, HC

    2014-06-11

    We demonstrate that rigidifying the structure of fluorescent linkers by structurally constraining them in metal-organic frameworks (MOFs) to control their conformation effectively tunes the fluorescence energy and enhances the quantum yield. Thus, a new tetraphenylethylene-based zirconium MOF exhibits a deep-blue fluorescent emission at 470 nm with a unity quantum yield (99.9 +/- 0.5%) under Ar, representing ca. 3600 cm(-1) blue shift and doubled radiative decay efficiency vs the linker precursor. An anomalous increase in the fluorescence lifetime and relative intensity takes place upon heating the solid MOF from cryogenic to ambient temperatures. The origin of these unusual photoluminescence properties is attributed to twisted linker conformation, intramolecular hindrance, and framework rigidity.

  5. Rigidifying Fluorescent Linkers by Metal–Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement

    SciTech Connect (OSTI)

    Wei, Zhangwen; Gu, Zhi-Yuan; Arvapally, Ravi K.; Chen, Ying-Pin; Ivy, Joshua F.; Yakovenko, Andrey A.; Feng, Dawei; Omary, Mohammad A.; Zhou, Hong-Cai

    2014-06-11

    We demonstrate that rigidifying the structure of fluorescent linkers by structurally constraining them in metal–organic frameworks (MOFs) to control their conformation effectively tunes the fluorescence energy and enhances the quantum yield. Thus, a new tetraphenylethylene-based zirconium MOF exhibits a deep-blue fluorescent emission at 470 nm with a unity quantum yield (99.9 ± 0.5%) under Ar, representing ca. 3600 cm?¹ blue shift and doubled radiative decay efficiency vs the linker precursor. An anomalous increase in the fluorescence lifetime and relative intensity takes place upon heating the solid MOF from cryogenic to ambient temperatures. The origin of these unusual photoluminescence properties is attributed to twisted linker conformation, intramolecular hindrance, and framework rigidity.

  6. DOE technical standards list. Department of Energy standards index

    SciTech Connect (OSTI)

    NONE

    1995-08-01

    This document was prepared for use by personnel involved in the selection and use of DOE technical standards and other Government and non-Government standards. This TSL provides listing of current DOE technical standards, non-Government standards that have been adopted by DOE, other Government documents in which DOE has a recorded interest, and canceled DOE technical standards. Information on new DOE technical standards projects, technical standards released for coordination, recently published DOE technical standards, and activities of non-Government standards bodies that may be of interest to DOE is published monthly in Standards Actions.

  7. DOE technical standards list: Department of Energy standards index

    SciTech Connect (OSTI)

    1999-05-01

    This Department of Energy (DOE) technical standards list (TSL) has been prepared by the Office of Nuclear Safety Policy and Standards (EH-31) on the basis of currently available technical information. Periodic updates of this TSL will be issued as additional information is received on standardization documents being issued, adopted, or canceled by DOE. This document was prepared for use by personnel involved in the selection and use of DOE technical standards and other Government and non-Government standards. This TSL provides listings of current DOE technical standards, non-Government standards that have been adopted by DOE, other standards-related documents in which DOE has a recorded interest, and canceled DOE technical standards. Information on new DOE technical standards projects, technical standards released for coordination, recently published DOE technical standards, and activities of non-Government standards bodies that may be of interest to DOE is published monthly in Standards Actions.

  8. Sonication standard laboratory module

    DOE Patents [OSTI]

    Beugelsdijk, Tony (Los Alamos, NM); Hollen, Robert M. (Los Alamos, NM); Erkkila, Tracy H. (Los Alamos, NM); Bronisz, Lawrence E. (Los Alamos, NM); Roybal, Jeffrey E. (Santa Fe, NM); Clark, Michael Leon (Menan, ID)

    1999-01-01

    A standard laboratory module for automatically producing a solution of cominants from a soil sample. A sonication tip agitates a solution containing the soil sample in a beaker while a stepper motor rotates the sample. An aspirator tube, connected to a vacuum, draws the upper layer of solution from the beaker through a filter and into another beaker. This beaker can thereafter be removed for analysis of the solution. The standard laboratory module encloses an embedded controller providing process control, status feedback information and maintenance procedures for the equipment and operations within the standard laboratory module.

  9. Drinking Water Standards 

    E-Print Network [OSTI]

    Dozier, Monty; McFarland, Mark L.

    2006-04-26

    This publication explains the federal safety standards for drinking water provided by public water supply systems. It discusses the legal requirements for public water supplies, the maximum level allowed for contaminants in the water...

  10. FREIGHT CONTAINER LIFTING STANDARD

    SciTech Connect (OSTI)

    POWERS DJ; SCOTT MA; MACKEY TC

    2010-01-13

    This standard details the correct methods of lifting and handling Series 1 freight containers following ISO-3874 and ISO-1496. The changes within RPP-40736 will allow better reading comprehension, as well as correcting editorial errors.

  11. Active DOE Technical Standards

    Energy Savers [EERE]

    with DOE Order 5480.23, Nuclear Safety Analysis Reports James O'Brien All Ac ve DOE Technical Standards Document Number Document Title Responsible SLM A-1 As of: 12-May-15...

  12. Standardization of Firearms

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2000-03-02

    Establishes Department of Energy (DOE) requirements for the standardization of firearms and limitations of firearms inventories maintained in support of safeguards and security activities. Does not cancel other directives.

  13. Building Energy Standards

    Broader source: Energy.gov [DOE]

    The 2015 Vermont Commercial Building Energy Standards (CBES) took effect on March 1, 2015. The code is based on the 2015 IECC, with amendments to incorporate ASHRAE 90.1-2013. The new guidelines ...

  14. Standard Offer Program

    Broader source: Energy.gov [DOE]

    Note: H.B. 40, enacted in June 2015, changes the name of the Sustainably Priced Energy Enterprise Development (SPEED) Program to the Standard Offer Program and replaces the associated state...

  15. EPCglobal : a universal standard

    E-Print Network [OSTI]

    Aguirre, Juan Ignacio

    2007-01-01

    This thesis evaluates the likelihood of EPCglobal becoming the universal RFID standard by presenting a framework of ten factors used to analyze and determine if EPCglobal is moving in the right direction. The ten factors ...

  16. Low Carbon Fuel Standards

    E-Print Network [OSTI]

    Sperling, Dan; Yeh, Sonia

    2009-01-01

    S O N I A YE H Low Carbon Fuel Standards The most direct andalternative transportation fuels is to spur innovation withstandard for upstream fuel producers. hen it comes to energy

  17. Measurement of plutonium in spent nuclear fuel by self-induced x-ray fluorescence

    SciTech Connect (OSTI)

    Hoover, Andrew S; Rudy, Cliff R; Tobin, Steve J; Charlton, William S; Stafford, A; Strohmeyer, D; Saavadra, S

    2009-01-01

    Direct measurement of the plutonium content in spent nuclear fuel is a challenging problem in non-destructive assay. The very high gamma-ray flux from fission product isotopes overwhelms the weaker gamma-ray emissions from plutonium and uranium, making passive gamma-ray measurements impossible. However, the intense fission product radiation is effective at exciting plutonium and uranium atoms, resulting in subsequent fluorescence X-ray emission. K-shell X-rays in the 100 keV energy range can escape the fuel and cladding, providing a direct signal from uranium and plutonium that can be measured with a standard germanium detector. The measured plutonium to uranium elemental ratio can be used to compute the plutonium content of the fuel. The technique can potentially provide a passive, non-destructive assay tool for determining plutonium content in spent fuel. In this paper, we discuss recent non-destructive measurements of plutonium X-ray fluorescence (XRF) signatures from pressurized water reactor spent fuel rods. We also discuss how emerging new technologies, like very high energy resolution microcalorimeter detectors, might be applied to XRF measurements.

  18. SAE Standards Support

    SciTech Connect (OSTI)

    Gowri, Krishnan

    2012-11-01

    This report summarizes PNNL activities in FY 2012 in support of the following two vehicle communication standards activities: • Technical support to SAE, ANSI and NIST technical working groups. PNNL actively contributed to the use case development, harmonization, and evaluation of the SAE standards activities for vehicle to grid communication • Tested and validated a set of potential technologies for meeting SAE communication requirements and provided recommendations for technology choices.

  19. Cyanine-based probe\\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

    SciTech Connect (OSTI)

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2013-01-15

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  20. Tracking Grantee Portal Standards and Recommendations Standards and Recommendations Table

    E-Print Network [OSTI]

    Tracking Grantee Portal Standards and Recommendations April 2009 Standards and Recommendations Table Tracking Network Standards Provide both public and secure grantee portals. Provide a link to the Tracking Network's National Portal from individual state portals. Maintain visual consistency among pages

  1. Tracking Grantee Portal Standards and Recommendations Standards and Recommendations Table

    E-Print Network [OSTI]

    Tracking Grantee Portal Standards and Recommendations April 2009 Standards and Recommendations Table Tracking Network Standards Provide both public and secure grantee portals. Provide a link on a grantee's portal. Organize contents of pages to facilitate the identification of detailed information

  2. Department of Energy Standards Index

    SciTech Connect (OSTI)

    1995-08-01

    This TSL, intended for use in selecting and using DOE technical standards and other Government and non-Government standards, provides listing of current and inactive DOE technical standards, non-Government standards adopted by DOE, other Government documents in which DOE has a recorded interest, and cancelled DOE technical standards.

  3. Fluorescent single walled nanotube/silica composite materials

    DOE Patents [OSTI]

    Dattelbaum, Andrew M.; Gupta, Gautam; Duque, Juan G.; Doorn, Stephen K.; Hamilton, Christopher E.; DeFriend Obrey, Kimberly A.

    2013-03-12

    Fluorescent composites of surfactant-wrapped single-walled carbon nanotubes (SWNTs) were prepared by exposing suspensions of surfactant-wrapped carbon nanotubes to tetramethylorthosilicate (TMOS) vapor. Sodium deoxycholate (DOC) and sodium dodecylsulphate (SDS) were the surfactants. No loss in emission intensity was observed when the suspension of DOC-wrapped SWNTs were exposed to the TMOS vapors, but about a 50% decrease in the emission signal was observed from the SDS-wrapped SWNTs nanotubes. The decrease in emission was minimal by buffering the SDS/SWNT suspension prior to forming the composite. Fluorescent xerogels were prepared by adding glycerol to the SWNT suspensions prior to TMOS vapor exposure, followed by drying the gels. Fluorescent aerogels were prepared by replacing water in the gels with methanol and then exposing them to supercritical fluid drying conditions. The aerogels can be used for gas sensing.

  4. Laser-induced differential normalized fluorescence method for cancer diagnosis

    DOE Patents [OSTI]

    Vo-Dinh, T.; Panjehpour, M.; Overholt, B.F.

    1996-12-03

    An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample. 5 figs.

  5. Laser-induced differential normalized fluorescence method for cancer diagnosis

    DOE Patents [OSTI]

    Vo-Dinh, Tuan (Knoxville, TN); Panjehpour, Masoud (Knoxville, TN); Overholt, Bergein F. (Knoxville, TN)

    1996-01-01

    An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.

  6. Engineering and Characterization of a Superfolder Green Fluorescent Protein

    SciTech Connect (OSTI)

    Pedelacq,J.; Cabantous, S.; Tran, T.; Terwilliger, T.; Waldo, G.

    2006-01-01

    Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

  7. High lumen compact fluorescents boost light output in new fixtures

    SciTech Connect (OSTI)

    1992-12-31

    Some compact fluorescent lamps aren`t so compact. General Electric (GE), OSRAM, and Philips have been expanding offerings in longer, more powerful, hard wired CFLs that generate enough light to serve applications once limited to conventional fluorescents and metal halide systems. All three of these manufacturers have for some time offered 18- to 40-watt high-output CFLs, which use a fluorescent tube doubled back on itself to produce a lot of light in a compact source. Now GE has introduced an even larger, more powerful 50-watt unit, and OSRAM is soon to follow suit with a 55-watt lamp. These new entries to the field of turbocharged CFLs can provide general lighting at ceiling heights of 12 feet or more as well as indirect lighting, floodlighting, and wall washing. They are such a concentrated source of light that they can provide the desired illumination using fewer lamps and fixtures than would be needed with competing sources.

  8. Nuclear Resonance Fluorescence for Safeguards Applications

    SciTech Connect (OSTI)

    Ludewigt, Bernhard A; Quiter, Brian J; Ambers, Scott D

    2011-02-04

    In nuclear resonance fluorescence (NRF) measurements, resonances are excited by an external photon beam leading to the emission of {gamma} rays with specific energies that are characteristic of the emitting isotope. The promise of NRF as a non-destructive analysis technique (NDA) in safeguards applications lies in its potential to directly quantify a specific isotope in an assay target without the need for unfolding the combined responses of several fissile isotopes as often required by other NDA methods. The use of NRF for detection of sensitive nuclear materials and other contraband has been researched in the past. In the safeguards applications considered here one has to go beyond mere detection and precisely quantify the isotopic content, a challenge that is discussed throughout this report. Basic NRF measurement methods, instrumentation, and the analytical calculation of NRF signal strengths are described in Section 2. Well understood modeling and simulation tools are needed for assessing the potential of NRF for safeguards and for designing measurement systems. All our simulations were performed with the radiation transport code MCNPX, a code that is widely used in the safeguards community. Our initial studies showed that MCNPX grossly underestimated the elastically scattered background at backwards angles due to an incorrect treatment of Rayleigh scattering. While new, corrected calculations based on ENDF form factors showed much better agreement with experimental data for the elastic scattering of photons on an uranium target, the elastic backscatter is still not rigorously treated. Photonuclear scattering processes (nuclear Thomson, Delbruck and Giant Dipole Resonance scattering), which are expected to play an important role at higher energies, are not yet included. These missing elastic scattering contributions were studied and their importance evaluated evaluated against data found in the literature as discussed in Section 3. A transmission experiment was performed in September 2009 to test and demonstrate the applicability of the method to the quantitative measurement of an isotope of interest embedded in a thick target. The experiment, data analysis, and results are described in Section 4. The broad goal of our NRF studies is to assess the potential of the technique in safeguards applications. Three examples are analyzed in Section 5: the isotopic assay of spent nuclear fuel (SNF), the measurement of {sup 235}U enrichment in UF{sub 6} cylinders, and the determination of {sup 239}Pu in mixed oxide (MOX) fuel. The study of NRF for the assay of SNF assemblies was supported by the Next Generation Safeguards Initiative (NGSI) of the U.S. Department of Energy as part of a large multi-lab/university effort to quantify the plutonium (Pu) mass in spent nuclear fuel assemblies and to detect the diversion of pins with non-destructive assay (NDA) methods. NRF is one of 14 NDA techniques being researched. The methodology for performing and analyzing quantitative NRF measurements was developed for determining Pu mass in SNF and is extensively discussed in this report. The same methodology was applied to the assessment of NRF for the measurement of {sup 235}U enrichment and the determination of {sup 239}Pu in MOX fuel. The analysis centers on determining suitable NRF measurement methods, measurement capabilities that could be realized with currently available instrumentation, and photon source and detector requirements for achieving useful NDA capabilities.

  9. DOE technical standards list: Department of Energy standards index

    SciTech Connect (OSTI)

    NONE

    1997-06-01

    This technical standards list (TSL) was prepared for use by personnel involved in the selection and use of US DOE technical standards and other government and non-government standards. This TSL provides listings of current DOE technical standards, non-government standards that have been adopted by DOE, other government documents in which DOE has a recorded interest, and cancelled DOE technical standards. Standards are indexed by type in the appendices to this document. Definitions of and general guidance for the use of standards are also provided.

  10. Commercialization of gallium nitride nanorod arrays on silicon for solid-state lighting

    E-Print Network [OSTI]

    Wee, Qixun

    2008-01-01

    One important component in energy usage is lighting, which is currently dominated by incandescent and fluorescent lamps. However, due to potentially higher efficiencies and thus higher energy savings, solid-state lighting ...

  11. Design and implementation of a solar power system in rural Haiti

    E-Print Network [OSTI]

    Hussam, Shaheer M. (Shaheer Muqtasid), 1981-

    2004-01-01

    This paper describes the design and implementation of a solar power system for a school and health center in Petit-Anse, Haiti. The end-use applications are lighting via a set of fluorescent and incandescent bulbs, and a ...

  12. School Energy Survey Student Guide

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of 40 new power plants. INCANDESCENT BULB HALOGEN COMPACT FLUORESCENT (CFL) LIGHT EMITTING DIODE (LED) Brightness 850 lumens 850 lumens 850 lumens 850 lumens Life of Bulb 1,000...

  13. School Energy Survey Teacher Guide

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Key Student Guide Page 20 INCANDESCENT BULB HALOGEN COMPACT FLUORESCENT (CFL) LIGHT EMITTING DIODE (LED) Number of bulbs to get 25,000 hours 25 8.3 2.5 1 Cost of bulbs for 25,000...

  14. Heat transfer assembly for a fluorescent lamp and fixture

    DOE Patents [OSTI]

    Siminovitch, M.J.; Rubenstein, F.M.; Whitman, R.E.

    1992-12-29

    In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure. 11 figs.

  15. Heat transfer assembly for a fluorescent lamp and fixture

    DOE Patents [OSTI]

    Siminovitch, Michael J. (Richmond, CA); Rubenstein, Francis M. (Berkeley, CA); Whitman, Richard E. (Richmond, CA)

    1992-01-01

    In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure.

  16. COORDINATION OF DOE TECHNICAL STANDARDS

    Broader source: Energy.gov [DOE]

    PurposeThis procedure provides guidance on the formal coordination of DOE Technical Standards in the DOE Technical Standards Program (TSP). The purpose of coordination of draft technical standards...

  17. Physics Beyond the Standard Model

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Beyond the Standard Model 1663 Los Alamos science and technology magazine Latest Issue:October 2015 past issues All Issues submit Physics Beyond the Standard Model...

  18. ISO 50001 Energy Management Standard

    SciTech Connect (OSTI)

    2013-08-12

    This powerful standard from the International Organization for Standardization (ISO) provides an internationally recognized framework for organizations to voluntarily implement an energy management system.

  19. MODEL CONSERVATION STANDARD INTRODUCTION

    E-Print Network [OSTI]

    financial assistance from the Bonneville Power Administration and the region's utilities. In addition of the plan by the Administrator, are directly attributable to such measure or resource. [Northwest Power ActMODEL CONSERVATION STANDARD INTRODUCTION As directed by the Northwest Power Act, the Council has

  20. Model conservation standards bibliography

    SciTech Connect (OSTI)

    Not Available

    1986-06-01

    This bibliography is divided into sections dealing with building design (superinsulation, solar houses, earth sheltered houses, heat loss calculation, lighting, retrofitting); heating, ventilation, and air conditioning; windows; doors; walls; roofs; floors; air leakage/infiltration; insulation materials; indoor air quality; moisture; performance; codes, laws, standards; economics; and program description. (DLC)

  1. Clothing Quality Standards 

    E-Print Network [OSTI]

    2006-05-01

    stream_source_info pdf_2298.pdf.txt stream_content_type text/plain stream_size 20810 Content-Encoding ISO-8859-1 stream_name pdf_2298.pdf.txt Content-Type text/plain; charset=ISO-8859-1 Clothing Quality Standards E-403...

  2. Derived Concentration Technical Standard

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-05-05

    This standard supports the implementation of Department of Energy (DOE) Order 458.1, Radiation Protection of the Public and the Environment. It also establishes the numerical values of DCSs in a manner reflecting the current state of knowledge and practice in radiation protection

  3. UCIT Data Network Standards Campus Wireless Network Standard

    E-Print Network [OSTI]

    Habib, Ayman

    UCIT Data Network Standards Campus Wireless Network Standard Classification IT Infrastructure Table.1. Standardization of the U of C's 802.11 wireless networks and frequency bands is required to insure availability of the wireless network to the campus community. Standards of use will improve the wireless service and help

  4. The Evolution of Vertical IS Standards: Electronic Interchange Standards

    E-Print Network [OSTI]

    Steinfield, Charles

    on the technical details of IT-related standards, surprisingly little empirical research addresses the development from horizontal standards, not only in their narrower applicability, but also in their technical conteThe Evolution of Vertical IS Standards: Electronic Interchange Standards in the US Home Mortgage

  5. DOE standard: Radiological control

    SciTech Connect (OSTI)

    Not Available

    1999-07-01

    The Department of Energy (DOE) has developed this Standard to assist line managers in meeting their responsibilities for implementing occupational radiological control programs. DOE has established regulatory requirements for occupational radiation protection in Title 10 of the Code of Federal Regulations, Part 835 (10 CFR 835), ``Occupational Radiation Protection``. Failure to comply with these requirements may lead to appropriate enforcement actions as authorized under the Price Anderson Act Amendments (PAAA). While this Standard does not establish requirements, it does restate, paraphrase, or cite many (but not all) of the requirements of 10 CFR 835 and related documents (e.g., occupational safety and health, hazardous materials transportation, and environmental protection standards). Because of the wide range of activities undertaken by DOE and the varying requirements affecting these activities, DOE does not believe that it would be practical or useful to identify and reproduce the entire range of health and safety requirements in this Standard and therefore has not done so. In all cases, DOE cautions the user to review any underlying regulatory and contractual requirements and the primary guidance documents in their original context to ensure that the site program is adequate to ensure continuing compliance with the applicable requirements. To assist its operating entities in achieving and maintaining compliance with the requirements of 10 CFR 835, DOE has established its primary regulatory guidance in the DOE G 441.1 series of Guides. This Standard supplements the DOE G 441.1 series of Guides and serves as a secondary source of guidance for achieving compliance with 10 CFR 835.

  6. Extending the utility of enzymes for site-specific targeting of fluorescent probes

    E-Print Network [OSTI]

    Liu, Daniel S. (Daniel Shao-Chen)

    2014-01-01

    Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, ...

  7. Detection of Ethylene Gas by Fluorescence Turn-On of a Conjugated Polymer

    E-Print Network [OSTI]

    Swager, Timothy Manning

    Ripe fruits: The fluorescence of a conjugated polymer is quenched by the presence of copper(I) moieties. Upon exposure to ethylene gas the copper complexes bind to ethylene and no longer quench the polymer fluorescence ...

  8. Cancer diagnostics using dynamic near-infrared optical imaging and fluorescent contrast agents 

    E-Print Network [OSTI]

    Gurfinkel, Mikhail

    2006-04-12

    A new optical imaging modality has been developed for small animal in vivo imaging of near-infrared fluorescence resulting from fluorescent contrast agents specifically targeted to molecular markers of cancer. The imaging ...

  9. Understanding Zinc Quantification with Existing and Advanced Ditopic Fluorescent Zinpyr Sensors

    E-Print Network [OSTI]

    Buccella, Daniela

    Treatment of aqueous zinc solutions with incremental additions of a ditopic fluorescent sensor of the Zinpyr family, based on pyridine/pyrazine-containing metal recognition units, affords a fluorescence titration curve ...

  10. Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology

    E-Print Network [OSTI]

    Krueger, Andrew T.

    Fluorescence spectroscopy is a powerful tool for probing complex biological processes. The ubiquity of peptide–protein and protein–protein interactions in these processes has made them important targets for fluorescence ...

  11. Microwave-Assisted Synthesis of Fluorescent Carbon Nanoparticles 

    E-Print Network [OSTI]

    Chen, Bonnie

    2014-08-19

    , such as buckminsterfullerene (C60)[5], carbon nanotubes (CNTs)[5], and nanodiamonds[5]. Their special chemical and physical properties point to a high potential in applications in industry and medicine. 1.3.2 Fluorescent carbon nano-particles discovered by accident...

  12. Identication of Major Water-Soluble Fluorescent Components of Some

    E-Print Network [OSTI]

    Myrick, Michael Lenn

    Polyaromatic hydrocarbons (PAHs) and other dissolved hydrocarbons are ubiquitous contaminants of seawater the pattern of ¯uorescent contaminants in water to be used as ®ngerprints of speci®c types of contaminationIdenti®cation of Major Water-Soluble Fluorescent Components of Some Petrochemicals M. GRONER, A. R

  13. Convection venting lensed reflector-type compact fluorescent lamp system

    DOE Patents [OSTI]

    Pelton, B.A.; Siminovitch, M.

    1997-07-29

    Disclosed herein is a fluorescent lamp housing assembly capable of providing convection cooling to the lamp and the ballast. The lens of the present invention includes two distinct portions, a central portion and an apertured portion. The housing assembly further includes apertures so that air mass is able to freely move up through the assembly and out ventilation apertures. 12 figs.

  14. Multicolor Fluorescent Biosensor for Multiplexed Detection of DNA Rong Hu,,,

    E-Print Network [OSTI]

    Tan, Weihong

    Engineering, College of Biology, Collaborative Innovation Center of Molecular Engineering for Theranostics 50-fold lower than that of traditional unamplified homogeneous fluorescent assay methods differences in drug metabo- lism.4,5 On the basis of their simplicity, low-cost, high sensitivity, rapid

  15. Fluorescence of silicon nanoparticles prepared by nanosecond pulsed laser

    SciTech Connect (OSTI)

    Liu, Chunyang, E-mail: chunyangliu@126.com; Sui, Xin; Yang, Fang; Ma, Wei; Li, Jishun; Xue, Yujun [Henan University of Science and Technology, Luoyang, 471003 (China)] [Henan University of Science and Technology, Luoyang, 471003 (China); Fu, Xing [Tianjin University, Tianjin, 300072 (China)] [Tianjin University, Tianjin, 300072 (China)

    2014-03-15

    A pulsed laser fabrication method is used to prepare fluorescent microstructures on silicon substrates in this paper. A 355 nm nanosecond pulsed laser micromachining system was designed, and the performance was verified and optimized. Fluorescence microscopy was used to analyze the photoluminescence of the microstructures which were formed using the pulsed laser processing technique. Photoluminescence spectra of the microstructure reveal a peak emission around 500 nm, from 370 nm laser irradiation. The light intensity also shows an exponential decay with irradiation time, which is similar to attenuation processes seen in porous silicon. The surface morphology and chemical composition of the microstructure in the fabricated region was also analyzed with multifunction scanning electron microscopy. Spherical particles are produced with diameters around 100 nm. The structure is compared with porous silicon. It is likely that these nanoparticles act as luminescence recombination centers on the silicon surface. The small diameter of the particles modifies the band gap of silicon by quantum confinement effects. Electron-hole pairs recombine and the fluorescence emission shifts into the visible range. The chemical elements of the processed region are also changed during the interaction between laser and silicon. Oxidation and carbonization play an important role in the enhancement of fluorescence emission.

  16. Optical gating of perylene bisimide fluorescence using dithienylcyclopentene photochromic switches

    SciTech Connect (OSTI)

    Pärs, Martti; Köhler, Jürgen, E-mail: juergen.koehler@uni-bayreuth.de [Experimental Physics IV, University of Bayreuth, 95440 Bayreuth (Germany)] [Experimental Physics IV, University of Bayreuth, 95440 Bayreuth (Germany); Gräf, Katja; Bauer, Peter; Thelakkat, Mukundan [Applied Functional Polymers, University of Bayreuth, 95440 Bayreuth (Germany)] [Applied Functional Polymers, University of Bayreuth, 95440 Bayreuth (Germany)

    2013-11-25

    The emission of millions of fluorescence photons from a chromophore is controlled by the absorption of a few tens of photons in a photochromic molecule. The parameters that determine the efficiency of this process are investigated, providing insights for the development of an all-optical gate.

  17. Plane-wave fluorescence tomography with adaptive finite elements

    E-Print Network [OSTI]

    Bangerth, Wolfgang

    Plane-wave fluorescence tomography with adaptive finite elements Amit Joshi Photon Migration reflectance imaging setup. The method employs planar illumination with modulated light and frequency domain fluo- rescence measurements made on the illumination plane. An adaptive finite-element algorithm

  18. Enhanced Archaerhodopsin Fluorescent Protein Voltage Yiyang Gong1,2*

    E-Print Network [OSTI]

    Ferrari, Silvia

    Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators Yiyang Gong1,2* , Jin Zhong Li1 by nearly three-fold in comparison to Arch-D95N. Citation: Gong Y, Li JZ, Schnitzer MJ (2013) Enhanced; Published June 19, 2013 Copyright: © 2013 Gong et al. This is an open-access article distributed under

  19. Experiment 4 Operation and Limitations of a Fluorescence Spectrometer

    E-Print Network [OSTI]

    Nazarenko, Alexander

    quinine concentration. Background: The principles of molecular fluorescence are covered in your text In this experiment, you will determine the exact amount of quinine in various liquids (tonic water and colas). The approximate concentration is 100 ppm. Preparation of solutions: 1.00 mM quinine in 0.05 M sulfuric acid

  20. EMERGING TECHNOLOGIES Two-photon excitation chlorophyll fluorescence lifetime imaging

    E-Print Network [OSTI]

    Qu, Jianan

    at higher Cd concentration was clearly identified. The decay of chlorophyll fluorescence extracted from) toxicity Abbreviations Cd Cadmium PS Photosystem LHCII Light-harvesting complex II PAM Pulse and Computer Engineering, Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon

  1. Fluorescent lamp with static magnetic field generating means

    DOE Patents [OSTI]

    Moskowitz, Philip E. (Peabody, MA); Maya, Jakob (Brookline, MA)

    1987-01-01

    A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed.

  2. Fluorescent lamp with static magnetic field generating means

    DOE Patents [OSTI]

    Moskowitz, P.E.; Maya, J.

    1987-09-08

    A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed. 2 figs.

  3. Amplifying Fluorescent Polymers Direct Synthesis of an OligonucleotidePoly-

    E-Print Network [OSTI]

    Tan, Weihong

    Amplifying Fluorescent Polymers Direct Synthesis of an Oligonucleotide­Poly- (phenylene ethynylene bioprobes can be constructed with these polymers for selective target recog- nition. To achieve this objective, the polymer must be conjugated with a biomolecule such as a DNA strand, a peptide, or a protein

  4. Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

    DOE Patents [OSTI]

    Weiss, Robert B. (Salt Lake City, UT); Kimball, Alvin W. (Salt Lake City, UT); Gesteland, Raymond F. (Salt Lake City, UT); Ferguson, F. Mark (Salt Lake City, UT); Dunn, Diane M. (West Valley City, UT); Di Sera, Leonard J. (Salt Lake City, UT); Cherry, Joshua L. (Salt Lake City, UT)

    1995-01-01

    A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots.

  5. Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy

    E-Print Network [OSTI]

    Rosen, Joseph

    Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy Gary, Israel 4 rosen@ee.bgu.ac.il *gbrooker@jhu.edu Abstract: Fresnel Incoherent Correlation Holography (FINCH. Rosen and G. Brooker, "Digital spatially incoherent Fresnel holography," Opt. Lett. 32(8), 912­914 (2007

  6. Purchasing Energy-Efficient Ceiling-Mounted Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    The Federal Energy Management Program (FEMP) provides acquisition guidance for ceiling-mounted fluorescent luminaires, a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR-qualified or FEMP-designated products in all product categories covered by these programs and in any acquisition actions that are not specifically exempted by law.

  7. Fluorescence photon migration by the boundary element method

    SciTech Connect (OSTI)

    Fedele, Francesco; Eppstein, Margaret J. . E-mail: maggie.eppstein@uvm.edu; Laible, Jeffrey P.; Godavarty, Anuradha; Sevick-Muraca, Eva M.

    2005-11-20

    The use of the boundary element method (BEM) is explored as an alternative to the finite element method (FEM) solution methodology for the elliptic equations used to model the generation and transport of fluorescent light in highly scattering media, without the need for an internal volume mesh. The method is appropriate for domains where it is reasonable to assume the fluorescent properties are regionally homogeneous, such as when using highly specific molecularly targeted fluorescent contrast agents in biological tissues. In comparison to analytical results on a homogeneous sphere, BEM predictions of complex emission fluence are shown to be more accurate and stable than those of the FEM. Emission fluence predictions made with the BEM using a 708-node mesh, with roughly double the inter-node spacing of boundary nodes as in a 6956-node FEM mesh, match experimental frequency-domain fluorescence emission measurements acquired on a 1087 cm{sup 3} breast-mimicking phantom at least as well as those of the FEM, but require only 1/8 to 1/2 the computation time.

  8. Throwing a wrench in the translational machinery : discovery of RNA ligands by fluorescence techniques

    E-Print Network [OSTI]

    Parsons, Jerod Russell

    2010-01-01

    1493 position had UV absorption measurements made at everyHX-HX5, Fluorescence measurements, UV melting experiments,

  9. Steady-state directional diffuse reflectance and fluorescence of human skin

    E-Print Network [OSTI]

    Pilon, Laurent

    .3660, 170.7050, 290.7050, 300.2530. 1. Introduction Fluorescence is the physical phenomenon in which light

  10. SO2 fluorescence from vacuum ultraviolet dissociative excitation of SO3

    SciTech Connect (OSTI)

    Suto, M.; Ye, C.; Ram, R.S.; Lee, L.C.

    1987-06-04

    The absorption and fluorescence excitation spectra of SO3 were measured in the 145-160-nm region with synchrotron radiation as a light source. The fluorescence spectra produced by excitation of SO3 at 147 and 157 nm were dispersed and compared with the laser-induced fluorescence spectrum of SO2, from which the emitter is identified to be the excited SO2*. The photodissociation process of SO3 is discussed in accord with the fluorescence observed.

  11. Underwater Lighting by Submerged Lasers and Incandescent Sources

    E-Print Network [OSTI]

    Duntley, Seibert Q

    1971-01-01

    case contains a rotary solenoid with its axle protrudingcylinder containing a rotary solenoid is mount- ed above theshown in Fig. 7-9 is a rotary solenoid which is a part This

  12. DOE Requires Manufacturer and Labeler to Cease Sale of Incandescent

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based| Department ofRefrigeratorsDepartmentEP9425

  13. How Energy-Efficient Light Bulbs Compare with Traditional Incandescents |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuelsof Energy ServicesContracting OversightEMSHome

  14. BOISE STATE UNIVERSITY CAMPUS STANDARDS

    E-Print Network [OSTI]

    Barrash, Warren

    experience. It addresses the look and feel of the physical environment by focusing on design elements1 BOISE STATE UNIVERSITY CAMPUS STANDARDS AND DESIGN GUIDELINES 1 CAMPUS STANDARDS AND DESIGN Standards and Design Guidelines are standard and enforceable design guidelines for all design

  15. 428 BIOCHIMICAET BIOPHYSICAACTA pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE

    E-Print Network [OSTI]

    Govindjee

    428 BIOCHIMICAET BIOPHYSICAACTA BBA 46126 pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE on the "slow" (min) time course of Chlorophyll a fluorescence yield in the green alga Chlorella pyrenoidosa and in the blue-green alga Anacystis nidulans. In Chlorella, the decay of fluorescence yield, in the I- to 5-rain

  16. Air fluorescence relevant for cosmic-ray detection--Review of pioneering measurements

    E-Print Network [OSTI]

    Hörandel, Jörg R.

    Air fluorescence relevant for cosmic-ray detection--Review of pioneering measurements Fernando Keywords: Fluorescence yield Air showers a b s t r a c t Cosmic rays with energies exceeding 1017 eV are frequently registered by measurements of the fluorescence light emitted by extensive air showers. The main

  17. Caged Molecular Fluorescence Velocimetry to measure meso-to micro-scale thermal flow fields 

    E-Print Network [OSTI]

    Park, Jaesung

    2000-01-01

    (CPL). The system utilizes a microscope objective lens, caged molecular fluorescence probes, Nd:YAG laser for UV light source, Ar-ion laser for 488 nm fluorescence pumping, and a color CCD camera to record a series of fluorescent images. Caged...

  18. Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins

    E-Print Network [OSTI]

    Campbell, Robert E.

    Proteins Hui-wang Ai, Nathan C. Shaner,§,| Zihao Cheng, Roger Y. Tsien,§ and Robert E. Campbell*, Uni: The variant of Aequorea green fluorescent protein (GFP) known as blue fluorescent protein (BFP) was originally improved versions of BFP along with a variety of engineered fluorescent protein variants with novel

  19. Standardized radiological dose evaluations

    SciTech Connect (OSTI)

    Peterson, V.L.; Stahlnecker, E.

    1996-05-01

    Following the end of the Cold War, the mission of Rocky Flats Environmental Technology Site changed from production of nuclear weapons to cleanup. Authorization baseis documents for the facilities, primarily the Final Safety Analysis Reports, are being replaced with new ones in which accident scenarios are sorted into coarse bins of consequence and frequency, similar to the approach of DOE-STD-3011-94. Because this binning does not require high precision, a standardized approach for radiological dose evaluations is taken for all the facilities at the site. This is done through a standard calculation ``template`` for use by all safety analysts preparing the new documents. This report describes this template and its use.

  20. Development of rapid microbial methods for lysine quantification in feed ingredients based on green fluorescent protein fluorescence 

    E-Print Network [OSTI]

    Chalova-Zhekova, Vesela I.

    2007-04-25

    ) was inserted in the genome of E. coli lysine auxotroph as a part of a mini-Tn5- transposon by conjugation. Bacterial growth response to external lysine and small peptides was monitored and recorded by measuring the fluorescence emitted by GFP. The second type...

  1. Standard Scenarios Annual Report

    Broader source: Energy.gov [DOE]

    The National Renewable Energy Laboratory is conducting a study sponsored by the U.S. Department of Energy DOE, Office of Energy Efficiency and Renewable Energy (EERE), that aims to document and implement an annual process designed to identify a realistic and timely set of input assumptions (e.g., technology cost and performance, fuel costs), and a diverse set of potential futures (standard scenarios), initially for electric sector analysis.

  2. Standard interface file handbook

    SciTech Connect (OSTI)

    Shapiro, A.; Huria, H.C. )

    1992-10-01

    This handbook documents many of the standard interface file formats that have been adopted by the US Department of Energy to facilitate communications between and portability of, various large reactor physics and radiation transport software packages. The emphasis is on those files needed for use of the VENTURE/PC diffusion-depletion code system. File structures, contents and some practical advice on use of the various files are provided.

  3. Standard Review Plan Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankADVANCED MANUFACTURINGEnergy BillsNo.Hydrogen4EnergySolidof2Standard Method of Test

  4. Seminar on Software Engineering Software Standards

    E-Print Network [OSTI]

    Feitelson, Dror

    APIs and protocols Process standards Quality standards Software engineering terminology Lifecycle- operability Certification, guidelines Assurances #12;Software Standards Quality standards are rare - HardSeminar on Software Engineering Software Standards #12;"the good thing about standards

  5. Standards Panel: 1. Stephen Diamond, General Manager, Industry Standards Office and Global Standards Officer, EMC

    E-Print Network [OSTI]

    Standards Panel: 1. Stephen Diamond, General Manager, Industry Standards Office and Global was President of the IEEE Computer Society. Steve is General Manager of the Industry Standards Office at EMC Standards Officer, EMC Corporation, Office of the CTO Steve Diamond has 30 years of management, marketing

  6. Standards of Conduct

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust,Field-effect Photovoltaics -7541C.3 SpecialSponsorJurczynski and HansStanStandards

  7. Title Standards 2001

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergyThe U.S.Laclede GasEfficiency| Department of Energy TheMeeksStandards 2001 A

  8. Standards | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LIST OF APPLICABLE DIRECTIVESDepartmentSpecialCode of Record Standard

  9. ORISE: Standards development

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesseworkSURVEY UNIVERSEHow ORISE is MakingScience EducationStandards

  10. Standard-E hydrogen monitoring system field acceptance testprocedure

    SciTech Connect (OSTI)

    Schneider, T.C.

    1997-02-01

    The purpose of this document is to demonstrate that the Standard-E Hydrogen Monitoring Systems (SHMS-E) installed on the Waste Tank Farms in the Hanford 200 Areas are constructed as intended by the design.

  11. The use of fluorescent compounds and complexes of metals as early warning detectors for corrosion

    SciTech Connect (OSTI)

    Johnson, R.E.; Agarwala, V.S.

    1994-12-31

    Several compounds have been identified which change their fluorescent behavior upon oxidation or reduction. These compounds have been investigated to determine the effects of temperature, pH, reduction conditions and re-oxidation behavior on their fluorescence. They have been incorporated into primer paint coatings for aluminum alloys and were found to fluoresce if the coating was scratched and exposed to air and moisture. Another compound was found to fluoresce with aluminum ions and its fluorescence behavior with varying conditions was also investigated. This compound, if added to a primer coating on an aluminum alloy, should fluoresce when aluminum ions begin to form as the alloy corrodes. Both of these methods appear to have potential to be early warning sensors for corrosion. Large surface areas of alloy material, such as are used in aircraft, could be scanned with UV light to detect the fluorescence of these materials indicating possible corrosion sites.

  12. Archaeometrical studies using X-ray fluorescence methods

    SciTech Connect (OSTI)

    Pauna, Catalina; Constantinescu, B.; Constantin, F.; Bugoi, R.; Stan, D.; Vasilescu, A. [National Institute of Nuclear Physics and Engineering, POB MG-6, 077125, Bucharest (Romania)

    2010-04-26

    Elemental analysis contributes to authentication (knowing the elemental composition and considering the information about the usual composition of the objects in different historical periods it can be established if the item is original or fake), provenance studies (minor and trace elements indicates ores origin and 'consequently' mines location), (relative) dating of archaeological objects (e.g. for painted items--the chemical recipes for pigments can offer information about the age of objects). The paper gives a general layout for the NIPNE Archaeometry Laboratory's applications using X-Ray Fluorescence (XRF), micro--Proton Induced X-Ray Emission (micro-PIXE), micro-Synchrotron Radiation Induced X-Ray Fluorescence (micro--SR-XRF) methods.

  13. Rotational order–disorder structure of fluorescent protein FP480

    SciTech Connect (OSTI)

    Pletnev, Sergei, E-mail: svp@ncifcrf.gov [SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Morozova, Kateryna S.; Verkhusha, Vladislav V. [Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461 (United States); Dauter, Zbigniew, E-mail: svp@ncifcrf.gov [Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States)

    2009-09-01

    An analysis of the rotational order–disorder structure of fluorescent protein FP480 is presented. In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

  14. Fluorescence quenching of CdSe quantum dots on graphene

    SciTech Connect (OSTI)

    Guo, Xi Tao; Hua Ni, Zhen, E-mail: zhni@seu.edu.cn; Yan Nan, Hai; Hui Wang, Wen [Department of Physics and Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 211189 (China)] [Department of Physics and Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 211189 (China); Yan Liao, Chun [Physics Department, National Photoelectric Technology and Functional Materials and Application of Science and Technology International Cooperation Base, Northwest University, Xi'an 710069 (China)] [Physics Department, National Photoelectric Technology and Functional Materials and Application of Science and Technology International Cooperation Base, Northwest University, Xi'an 710069 (China); Zhang, Yan; Wei Zhao, Wei [Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189 (China)] [Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189 (China)

    2013-11-11

    We studied systematically the fluorescence quenching of CdSe quantum dots (QDs) on graphene and its multilayers, as well as graphene oxide (GO) and reduced graphene oxide (rGO). Raman intensity of QDs was used as a quantitatively measurement of its concentration in order to achieve a reliable quenching factor (QF). It was found that the QF of graphene (?13.1) and its multilayers is much larger than rGO (?4.4), while GO (?1.5) has the lowest quenching efficiency, which suggests that the graphitic structure is an important factor for quenching the fluorescence of QDs. It was also revealed that the QF of graphene is not strongly dependent on its thicknesses.

  15. Blue fluorescent organic light emitting diodes with multilayered graphene anode

    SciTech Connect (OSTI)

    Hwang, Joohyun [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Choi, Hong Kyw [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Moon, Jaehyun; Shin, Jin-Wook; Joo, Chul Woong; Han, Jun-Han; Cho, Doo-Hee; Huh, Jin Woo [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Choi, Sung-Yool [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Lee, Jeong-Ik, E-mail: jiklee@etri.re.kr [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Chu, Hye Yong [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)

    2012-10-15

    As an innovative anode for organic light emitting devices (OLEDs), we have investigated graphene films. Graphene has importance due to its huge potential in flexible OLED applications. In this work, graphene films have been catalytically grown and transferred to the glass substrate for OLED fabrications. We have successfully fabricated 2 mm × 2 mm device area blue fluorescent OLEDs with graphene anodes which showed 2.1% of external quantum efficiency at 1000 cd/m{sup 2}. This is the highest value reported among fluorescent OLEDs using graphene anodes. Oxygen plasma treatment on graphene has been found to improve hole injections in low voltage regime, which has been interpreted as oxygen plasma induced work function modification. However, plasma treatment also increases the sheet resistance of graphene, limiting the maximum luminance. In summary, our works demonstrate the practical possibility of graphene as an anode material for OLEDs and suggest a processing route which can be applied to various graphene related devices.

  16. A discussion SuStainability StandardS

    E-Print Network [OSTI]

    A discussion SuStainability StandardS in China­latin ameriCa trade and inveStment Emma Blackmor@iied.org, www.iied.org/pubs Citation Blackmore, E. Li, D., and Casallas, S. (2013). Sustainability standards.regent-typesetting.co.uk Photo credit Simon Lim Edited by Nina Berhman Series Editor Emma Blackmore Shaping Sustainable Markets

  17. New standard exceeds expectations

    SciTech Connect (OSTI)

    Bennett, M.J. (Environmental Data Resources Inc., Southport, CT (United States))

    1993-08-01

    The new ASTM environmental due diligence standard is delivering far more than expected when it was conceived in 1990. Its use goes well beyond the relatively narrow legal liability protection that was the primary goal in its development. The real estate industry, spearheaded by the lending community, was preoccupied with environmental risk and liability. Lenders throughout the concept's evolution have been at the forefront in defining environmental due diligence. The lender liability rule is intended to protect property owners from CERCLA liability for property they own or companies they manage (for example, as a result of foreclosure). The new site assessment standard increasingly is considered a benchmark for prudent environmental due diligence in the interest of risk management, not legal liability. The focus on risk management, including collateral devaluation and corporate credit risk, are becoming dominant areas of policy focus in the lending industry. Lenders now are revising their policies to incorporate transactions beyond issues of real estate, in which a company's economic viability and ability to service debt could be impacted by an environmental problem unrelated to property transfers.

  18. X-Ray Fluorescence (XRF) | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-EnhancingEtGeorgia:Illinois:Wizard PowerWyandanch, New1991) | Open EnergyFluorescence

  19. Analysis and design of an electronic ballast for fluorescent tube 

    E-Print Network [OSTI]

    Kuchimanchi, Krishna V.

    1992-01-01

    Chair of Advisory Committee: Dr. Enjeti Prasad In this thesis, the analysis, design and implementation of an electronic ballast circuit with power factor correction to replace the conventional ballast for fluorescent lighting systems is presented... layout for the PCB Voltage across the tube Current through the tube 44 45 25 Gate voltages across the mosfets 27 Conventional diode rectifier Rectifier with inductor 47 48 28 Series resonant filter 49 29 Parallel resonant power factor...

  20. Contraband Detection with Nuclear Resonance Fluorescence: Feasibility and Impact

    SciTech Connect (OSTI)

    Pruet, J; Lange, D

    2007-01-03

    In this report they show that cargo interrogation systems developed to thwart trafficking of illicit nuclear materials could also be powerful tools in the larger fight against contraband smuggling. In particular, in addition to detecting special nuclear materials, cargo scanning systems that exploit nuclear resonance fluorescence to detect specific isotopes can be used to help find: chemical weapons; some drugs as well as some chemicals regulated under the controlled substances act; precious metals; materials regulated under export control laws; and commonly trafficked fluorocarbons.

  1. Fluorescent spectra of chromatographic fractions of crude oils 

    E-Print Network [OSTI]

    Dixon, William Samuel

    1952-01-01

    of Results, ~ ~ ~ ~ ~ ~ i ~ ~ ~ ~ 25 XI. Bibliography. ~ . "" o" . . ". .. . . . ~ 26 XII. Appendix PURPOSE This invest1gation ?as undertaken in an effort to develop a means of the chromatographic separation of a crude oil~ and to examine... these fractions by spectro;ra hic means to determines (l) v'hether there are differences in the fluorescent spectra of the various chromatographic fractions oi a given crude oil, and (2) whether there are differ- ences between similar chromatographic fractions...

  2. Enhanced detection of fluorescence quenching in labeled cells

    DOE Patents [OSTI]

    Crissman, H.A.; Steinkamp, J.A.

    1987-11-30

    A method is provided for quantifying BrdU labeled DNA in cells. The BrdU is substituted onto the DNA and the DNA is stained with a first fluorochrome having a fluorescence which is quenchable by BrdU. The first fluorochrome is preferably a thymidine base halogen analogue, such as a Hoechst fluorochrome. The DNA is then stained with a second fluorochrome having a fluorescence which is substantially uneffected by BrdU. The second fluorochrome may be selected from the group consisting of mithramycin, chromomycin A3, olivomycin, propidium iodide and ethidium bromine. The fluorescence from the first and second fluorochromes is then measured to obtain first and second output signals, respectively. The first output signal is subtracted from the second output signal to obtain a difference signal which is functionally related to the quantity of BrdU incorporated into DNA. The technique is particularly useful for quantifying the synthesis of DNA during the S-phase of the cell cycle. 2 figs.

  3. Standard Templates Adaptive Parallel Library 

    E-Print Network [OSTI]

    Arzu, Francisco Jose

    2000-01-01

    STAPL (Standard Templates Adaptive Parallel Library) is a parallel C++ library designed as a superset of the C++ Standard Template Library (STL), sequentially consistent for functions with the same name, and executed on uni- or multi- processor...

  4. Energy Standards for State Buildings

    Office of Energy Efficiency and Renewable Energy (EERE)

    The State is still required by statute to adopt planning and construction standards for state buildings that conserve energy and optimize the energy performance of new buildings. The standards mu...

  5. Energy Efficiency Standards for Appliances

    Broader source: Energy.gov [DOE]

    In 2007 the District of Columbia (D.C.) enacted legislation, entitled the Energy Efficiency Standards Act of 2007, which created efficiency standards for six products, four of which were...

  6. Energy Efficiency Standards for Appliances

    Broader source: Energy.gov [DOE]

    Connecticut enacted efficiency standards through legislative actions in 2004 and 2007 and 2011. This law covers the following products that have not been pre-empted by federal standards:...

  7. Laboratory-based micro-X-ray fluorescence setup using a von Hamos crystal spectrometer and a focused beam X-ray tube

    SciTech Connect (OSTI)

    Kayser, Y.; B?achucki, W.; Dousse, J.-Cl.; Hoszowska, J.; Neff, M.; Romano, V.

    2014-04-15

    The high-resolution von Hamos bent crystal spectrometer of the University of Fribourg was upgraded with a focused X-ray beam source with the aim of performing micro-sized X-ray fluorescence (XRF) measurements in the laboratory. The focused X-ray beam source integrates a collimating optics mounted on a low-power micro-spot X-ray tube and a focusing polycapillary half-lens placed in front of the sample. The performances of the setup were probed in terms of spatial and energy resolution. In particular, the fluorescence intensity and energy resolution of the von Hamos spectrometer equipped with the novel micro-focused X-ray source and a standard high-power water-cooled X-ray tube were compared. The XRF analysis capability of the new setup was assessed by measuring the dopant distribution within the core of Er-doped SiO{sub 2} optical fibers.

  8. TECHNICAL STANDARDS PROGRAM TOPICAL COMMITTEES

    Broader source: Energy.gov [DOE]

    PurposeThis procedure describes how topical committees are organized and recognized under the Technical Standards Program. 

  9. Environmental Public Health Performance Standards

    E-Print Network [OSTI]

    Environmental Public Health Performance Standards (Version 2.0) Updated May 2014 National Center for Environmental Health Division of Emergency and Environmental Health Services #12;#12;Environmental Public Health Performance Standards (Version 2.0) Updated May 2014 #12;Environmental Public Health Performance Standards

  10. AN UPDATE ON CRYPTOGRAPHIC STANDARDS,

    E-Print Network [OSTI]

    has worked actively with other government and industry organizations to develop standards government and industry organizations to develop standards and guidelines for the cost-effective usesMay 2006 AN UPDATE ON CRYPTOGRAPHIC STANDARDS, GUIDELINES, AND TESTING REQUIREMENTS AN UPDATE

  11. China Cools with Tighter RAC Standards

    E-Print Network [OSTI]

    Lin, Jiang; Rosenquist, Gregory

    2006-01-01

    standards on energy savings, electric generation capacity,standards on energy savings, electric generation capacity,

  12. Technical Standards Newsletter - September 2004 | Department...

    Broader source: Energy.gov (indexed) [DOE]

    The Standards Forum and Standards Actions - September 2004 Inside this issue: TSP Manager's Note... 1 Pollution...

  13. Non standard neutrino interactions

    E-Print Network [OSTI]

    Miranda, O G

    2015-01-01

    Neutrino oscillations have become well-known phenomenon; the measurements of neutrino mixing angles and mass squared differences are continuously improving. Future oscillation experiments will eventually determine the remaining unknown neutrino parameters, namely, the mass ordering, normal or inverted, and the CP-violating phase. On the other hand, the absolute mass scale of neutrinos could be probed by cosmological observations, single beta decay as well as by neutrinoless double beta decay experiments. Furthermore, the last one may shed light on the nature of neutrinos, Dirac or Majorana, by measuring the effective Majorana mass of neutrinos. However, the neutrino mass generation mechanism remains unknown. A well-motivated phenomenological approach to search for new physics, in the neutrino sector, is that of non-standard interactions. In this short review, the current constraints in this picture, as well as the perspectives from future experiments, are discussed.

  14. ARM Standards Policy Committee Report

    SciTech Connect (OSTI)

    Cialella, A; Jensen, M; Koontz, A; McFarlane, S; McCoy, R; Monroe, J; Palanisamy, G; Perez, R; Sivaraman, C

    2012-09-19

    Data and metadata standards promote the consistent recording of information and are necessary to ensure the stability and high quality of Atmospheric Radiation Measurement (ARM) Climate Research Facility data products for scientific users. Standards also enable automated routines to be developed to examine data, which leads to more efficient operations and assessment of data quality. Although ARM Infrastructure agrees on the utility of data and metadata standards, there is significant confusion over the existing standards and the process for allowing the release of new data products with exceptions to the standards. The ARM Standards Policy Committee was initiated in March 2012 to develop a set of policies and best practices for ARM data and metadata standards.

  15. Environmental standards provide competitive advantage

    SciTech Connect (OSTI)

    Chynoweth, E.; Kirshner, E.

    1993-04-28

    Quality organizations are breaking new ground with the development of international standards for environmental management. These promise to provide the platform for chemical companies wanting to establish their environmental credibility with a global audience. [open quotes]It will be similar to auditing our customers to ISO 9000[close quote], says the environmental manager for a European chemical firm. [open quote]We will only want to deal with people who have got their environmental act together. And we'll be in a better competitive positions[close quote]. The International Organization for Standardization (ISO;Geneva) has set up a taskforce to develop an environmental management standard, which is expected to be completed by the mid-1990s. Observers think the ISO standard will draw heavily on the British Standard Institute's (BSI;London) environmental management standard, BS7750, which will likely be the first system adopted in the world. Published last year, BS7750 has been extensively piloted in the UK (CW, Sept. 30, 1992, p. 62) and is now set to be revised before being offically adopted by BSI. The UK's Chemical Industries Association (CIA;London) is anxious to prevent a proliferation of standards, and its report on BS7750 pilot projects calls for an approach integrating quality, environment, and health and safety. But standard setters, including ISO, appear to be moving in the opposite direction. In the US, the American national Standards Institute (ANSI;Washington) has started work on an environmental management standard.

  16. Calculation of K-shell fluorescence yields for low-Z elements

    SciTech Connect (OSTI)

    Nekkab, M.; Kahoul, A.; Deghfel, B.; Aylikci, N. Küp; Aylikçi, V.

    2015-03-30

    The analytical methods based on X-ray fluorescence are advantageous for practical applications in a variety of fields including atomic physics, X-ray fluorescence surface chemical analysis and medical research and so the accurate fluorescence yields (?{sub K}) are required for these applications. In this contribution we report a new parameters for calculation of K-shell fluorescence yields (?{sub K}) of elements in the range of 11?Z?30. The experimental data are interpolated by using the famous analytical function (?{sub k}/(1??{sub k})){sup 1/q} (were q=3, 3.5 and 4) vs Z to deduce the empirical K-shell fluorescence yields. A comparison is made between the results of the procedures followed here and those theoretical and other semi-empirical fluorescence yield values. Reasonable agreement was typically obtained between our result and other works.

  17. Evaluating the variability of ceramics with x-ray fluorescence

    SciTech Connect (OSTI)

    Crown, P.L.; Schwalbe, L.A.; London, J.R.

    1984-01-01

    Our assessment of prehistoric trade in ceramics depends on our ability to identify and distinguish different sources of manufacture. For the American Southwest, archaeologists have proposed various models of ceramic manufacture and exchange. Until recently, conflicting hypotheses were tested mainly on the basis of petrographic analysis of nonplastic tempering materials. We have extended these analyses to include x-ray fluorescence (XRF). XRF provides a fast and inexpensive means of analyzing large numbers of samples. Since 1982, approximately 500 prehistoric sherds and 40 prepared clay and mineral samples have been examined with XRF. Multivariate statistical techniques have been applied to help identify groupings of samples with possible archaeological significance.

  18. Blue-green phosphor for fluorescent lighting applications

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  19. Rapid identification of bacteria using an umbelliferone fluorescent assay 

    E-Print Network [OSTI]

    Chamblin, Richard Thomas

    1983-01-01

    . Corgnebacterium seudotuberculosis KF which formed hard, dry colonies; or sr 1 11 ~11' LM h1*h f 4 * pt' lly " t"c colonies), 2 loopfulls of' bacteria per ml were estimated. Estimating 2 loopfulls proved to be an acceptable variation in the technique. Wet.... Quarles ( Co-Chairman of Committee) Charles F. Hall ( ember) L. C. Grumbles ( Member) B. G. Foster Head of Depar t) Ian R. Tiz r August 1983 ABSTRACT Rapid Identification of Bacteria Using an Umbelliferone Fluorescent Assay, (August 1983) Richard...

  20. A Fluorescent Approach for Identifying P2X1 Ligands

    E-Print Network [OSTI]

    Ruepp, Marc-David; Brozik, James A.; de Esch, Iwan J. P.; Farndale, Richard W.; Murrell-Lagnado, Ruth D.; Thompson, Andrew J.

    2015-05-28

    by Richard Evans (Leicester University, UK). Plasmids: A FLAG-tagged human P2X1 subunit expression construct was created by fusion PCR. The fragment was cloned together with an IRES-IFP1.4 (Shu et al., Science 2009; 324 no.5928) fusion PCR product... 4 ligands, supporting our notion that fluorescence-based methods provide a valuable research tool (Hernandez-Olmos et al., 2012). Other methods such as surface 19 plasmon resonance (SPR), nuclear magnetic resonance (NMR), X-ray structure...

  1. Rigidifying Fluorescent Linkers by Metal-Organic Framework Formation for

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid youOxygen Generation |Publications|RetireeTechnologiesFluorescence Blue

  2. Better Fluorescent Lighting Through Physics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuels DataEnergy Webinar:I DueBETO Quiz -Technologies for HybridBetterFluorescent

  3. Air Fluorescence Relevant for Cosmic-Ray Detection - Review of Pioneering Measurements

    E-Print Network [OSTI]

    Fernando Arqueros; Joerg R. Hoerandel; Bianca Keilhauer

    2008-07-24

    Cosmic rays with energies exceeding $10^{17}$ eV are frequently registered by measurements of the fluorescence light emitted by extensive air showers. The main uncertainty for the absolute energy scale of the measured air showers is coming from the fluorescence light yield of electrons in air. The fluorescence light yield has been studied in laboratory experiments. Pioneering measurements between 1954 and 2000 are reviewed.

  4. Microcavity effects on the generation,fluorescence, and diffusion of excitons in organic solar cells

    E-Print Network [OSTI]

    Kozyreff, G; Vuong, L T; Silleras, O Nieto; Martorell, J

    2013-01-01

    We compute the short-circuit diffusion current of excitons in an organic solar cell, with special emphasis on fluorescence losses. The exciton diffusion length is not uniform but varies with its position within the device, even with moderate fluorescence quantum efficiency. With large quantum efficiencies, the rate of fluorescence can be strongly reduced with proper choices of the geometrical and dielectric parameters. In this way, the diffusion length can be increased and the device performance significantly improved.

  5. Analysis of federal policy options for improving US lighting energy efficiency: Commercial and residential buildings

    SciTech Connect (OSTI)

    Atkinson, B.A.; McMahon, J.E.; Mills, E.; Chan, P.; Chan, T.W.; Eto, J.H.; Jennings, J.D.; Koomey, J.G.; Lo, K.W.; Lecar, M.; Price, L.; Rubinstein, F.; Sezgen, O.; Wenzel, T.

    1992-12-01

    The US Department of Energy (DOE) has recognized the opportunity to achieve energy, economic, and environmental benefits by promoting energy-efficient lighting through federal policies, including lighting standards, financial incentives, and information programs. To assist in this process, the Office of Conservation and Renewable Energy`s Office of Codes and Standards invited Lawrence Berkeley Laboratory to assess prospective national impacts for a variety of policy options. Some progress has already been made in developing lighting policies at both the federal and state levels. The US DOE`s Office of Building Technologies has evaluated lighting efficiency incentives as part of its analysis for the National Energy Strategy. Fluorescent and incandescent lamp standards are included in the national Energy Policy Act of 1992 (P.L. 102-486, October 24, 1992). A few states have analyzed or implemented lamp and luminaire standards. Many policy-related issues merit further investigation. For example, there is considerable debate over issues such as mandatory or voluntary standards versus component labeling and other education-oriented strategies. Several different technologies are involved that interact with each other-lamps (incandescent, compact fluorescent, and HID), ballasts (for fluorescent and HID lamps), and fixtures with reflectors and lenses. Control systems and operation patterns must also be considered (timers, automated dimming, or occupancy sensors). Lighting applications are diverse, ranging from offices, restaurants, hallways, hospital operating rooms, to exterior lights. Lighting energy use influences heating and cooling requirements in buildings. Successful lighting system design must also address interactions between architectural design elements and daylighting availability. Proper system installation and ongoing operation and maintenance are crucial. The economic aspects of the preceding points must also be considered for policy making.

  6. Analysis of federal policy options for improving US lighting energy efficiency: Commercial and residential buildings

    SciTech Connect (OSTI)

    Atkinson, B.A.; McMahon, J.E.; Mills, E.; Chan, P.; Chan, T.W.; Eto, J.H.; Jennings, J.D.; Koomey, J.G.; Lo, K.W.; Lecar, M.; Price, L.; Rubinstein, F.; Sezgen, O.; Wenzel, T.

    1992-12-01

    The US Department of Energy (DOE) has recognized the opportunity to achieve energy, economic, and environmental benefits by promoting energy-efficient lighting through federal policies, including lighting standards, financial incentives, and information programs. To assist in this process, the Office of Conservation and Renewable Energy's Office of Codes and Standards invited Lawrence Berkeley Laboratory to assess prospective national impacts for a variety of policy options. Some progress has already been made in developing lighting policies at both the federal and state levels. The US DOE's Office of Building Technologies has evaluated lighting efficiency incentives as part of its analysis for the National Energy Strategy. Fluorescent and incandescent lamp standards are included in the national Energy Policy Act of 1992 (P.L. 102-486, October 24, 1992). A few states have analyzed or implemented lamp and luminaire standards. Many policy-related issues merit further investigation. For example, there is considerable debate over issues such as mandatory or voluntary standards versus component labeling and other education-oriented strategies. Several different technologies are involved that interact with each other-lamps (incandescent, compact fluorescent, and HID), ballasts (for fluorescent and HID lamps), and fixtures with reflectors and lenses. Control systems and operation patterns must also be considered (timers, automated dimming, or occupancy sensors). Lighting applications are diverse, ranging from offices, restaurants, hallways, hospital operating rooms, to exterior lights. Lighting energy use influences heating and cooling requirements in buildings. Successful lighting system design must also address interactions between architectural design elements and daylighting availability. Proper system installation and ongoing operation and maintenance are crucial. The economic aspects of the preceding points must also be considered for policy making.

  7. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, Ryszard J. (Ames, IA); Small, Gerald J. (Ames, IA); Shields, Peter A. (Reading, MA)

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.

  8. Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection of Small Molecules

    E-Print Network [OSTI]

    Tan, Weihong

    Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection graphene oxide (GO) as the signal amplifier. Because of the extraordinarily larger volume of GO

  9. Transmission Nuclear Resonance Fluorescence Measurements of 238U in Thick Targets

    E-Print Network [OSTI]

    Quiter, Brian J.

    2011-01-01

    G. Rusev, and A.P. Tonchev, Transmission-based detection ofas it traverses the transmission detection sheet. The secondTransmission Nuclear Resonance Fluorescence Measurements of

  10. Excitation-emission spectra and fluorescence quantum yields for fresh and aged biogenic secondary organic aerosols

    SciTech Connect (OSTI)

    Lee, Hyun Ji; Laskin, Alexander; Laskin, Julia; Nizkorodov, Sergey A.

    2013-05-10

    Certain biogenic secondary organic aerosols (SOA) become absorbent and fluorescent when exposed to reduced nitrogen compounds such as ammonia, amines and their salts. Fluorescent SOA may potentially be mistaken for biological particles by detection methods relying on fluorescence. This work quantifies the spectral distribution and effective quantum yields of fluorescence of SOA generated from two monoterpenes, limonene and a-pinene, and two different oxidants, ozone (O3) and hydroxyl radical (OH). The SOA was generated in a smog chamber, collected on substrates, and aged by exposure to ~100 ppb ammonia vapor in air saturated with water vapor. Absorption and excitation-emission matrix (EEM) spectra of aqueous extracts of aged and control SOA samples were measured, and the effective absorption coefficients and fluorescence quantum yields (~0.005 for 349 nm excitation) were determined from the data. The strongest fluorescence for the limonene-derived SOA was observed for excitation = 420+- 50 nm and emission = 475 +- 38 nm. The window of the strongest fluorescence shifted to excitation = 320 +- 25 nm and emission = 425 +- 38 nm for the a-pinene-derived SOA. Both regions overlap with the excitation-emission matrix (EEM) spectra of some of the fluorophores found in primary biological aerosols. Our study suggests that, despite the low quantum yield, the aged SOA particles should have sufficient fluorescence intensities to interfere with the fluorescence detection of common bioaerosols.

  11. High power light emitting diode based setup for photobleaching fluorescent impurities

    E-Print Network [OSTI]

    Kaufman, Laura

    High power light emitting diode based setup for photobleaching fluorescent impurities Tobias K be photobleached before final sample preparation. The instrument consists of high power light emitting diodes

  12. Fluorescence-based detection methodologies for nitric oxide using transition metal scaffolds

    E-Print Network [OSTI]

    Hilderbrand, Scott A. (Scott Alan), 1976-

    2004-01-01

    Chapter 1. Fluorescence-Based Detection Methodologies for Nitric Oxide: A Review. Chapter 2. Cobalt Chemistry with Mixed Aminotroponimine Salicylaldimine Ligands: Synthesis, Characterization, and Nitric Oxide Reactivity. ...

  13. Lung Cancer (2005) 47, 41--47 Improving the specificity of fluorescence

    E-Print Network [OSTI]

    Duin, Robert P.W.

    2005-01-01

    Lung Cancer (2005) 47, 41--47 Improving the specificity of fluorescence bronchoscopy spectroscopy; Lung cancer; Bronchoscopy Summary Detection of malignancies of the bronchial tree in an early

  14. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, R.J.; Small, G.J.; Shields, P.A.

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

  15. Nuclear Resonance Fluorescence at MIT | U.S. DOE Office of Science...

    Office of Science (SC) Website

    Resonance Fluorescence at MIT Nuclear Physics (NP) NP Home About Research Facilities Science Highlights Benefits of NP Applications of Nuclear Science Applications of Nuclear...

  16. Nuclear Resonance Fluorescence to Measure Plutonium Mass in Spent Nuclear Fuel

    E-Print Network [OSTI]

    Ludewigt, Bernhard A

    2011-01-01

    and Nuclear Recoil . . . . . . . . . . . . . . . . . . . . .2 Quantitative Measurements using NRF 2.1 Nuclear ResonanceFuture Work A Transmission Nuclear Resonance Fluorescence

  17. Anatomy-Based Algorithms for Detecting Oral Cancer Using Reflectance and Fluorescence Spectroscopy

    E-Print Network [OSTI]

    McGee, Sasha

    OBJECTIVES: We used reflectance and fluorescence spectroscopy to noninvasively and quantitatively distinguish benign from dysplastic/malignant oral lesions. We designed diagnostic algorithms to account for differences in ...

  18. Fluorescence spectrum analysis using Fourier series modeling for Fluorescein solution in Ethanol

    E-Print Network [OSTI]

    Hadi, Mahasin F

    2011-01-01

    We have measured the fluorescence spectrum for fluorescein solution in ethanol with concentration 1 {\\times} 10-3 mol/liter at different temperatures from room temperature to freezing point of solvent, (T = 153, 183, 223, 253, and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has been used to determine the fitting curve and fitting equation for each fluorescence spectrum. Fourier series (3 {\\times} 2) was the most suitable fitting equation for all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol at T = 183K was calculated and compared with experimental fluorescence spectrum at the same temperature. There is a good similarity between them.

  19. Diagnosing breast cancer using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy

    E-Print Network [OSTI]

    Fitzmaurice, Maryann

    Using diffuse reflectance spectroscopy and intrinsic fluorescence spectroscopy, we have developed an algorithm that successfully classifies normal breast tissue, fibrocystic change, fibroadenoma, and infiltrating ductal ...

  20. Quantum-jumps and photon-statistic in fluorescent systems coupled to classically fluctuating reservoirs

    E-Print Network [OSTI]

    Adrian A. Budini

    2010-05-20

    In this paper, we develop a quantum-jump approach for describing the photon-emission process of single fluorophore systems coupled to complex classically fluctuating reservoirs. The formalism relies on an open quantum system approach where the dynamic of the system and the reservoir fluctuations are described through a density matrix whose evolution is defined by a Lindblad rate equation. For each realization of the photon measurement processes it is possible to define a conditional system state (stochastic density matrix) whose evolution depends on both the photon detection events and the fluctuations between the configurational states of the reservoir. In contrast to standard fluorescent systems the photon-to-photon emission process is not a renewal one, being defined by a (stochastic) waiting time distribution that in each recording event parametrically depends on the conditional state. The formalism allows calculating experimental observables such as the full hierarchy of joint probabilities associated to the time intervals between consecutive photon recording events. These results provide a powerful basis for characterizing different situations arising in single-molecule spectroscopy, such as spectral fluctuations, lifetime fluctuations, and light assisted processes.

  1. Project Analysis Standard Operating Procedure

    Office of Environmental Management (EM)

    Standard Operating Procedure (EPASOP) Issued by Office of Acquisition and Project Management MA-63 March 12, 2014 DEPARTMENT OF ENERGY Office of Acquisition and Project...

  2. web identity standards introduction .............................................................................................................87

    E-Print Network [OSTI]

    Mullins, Dyche

    web identity standards sectionten 86 contents introduction............................................................................................................... 89-90 tagline and the web .................................................................................................91 tagline and the web: improper use

  3. Modeling Gaps and Overlaps of Sustainability Standards

    E-Print Network [OSTI]

    D'Alessio, Anna E.; Witherell, Paul; Rachuri, Sudarsan

    2012-01-01

    the standards and an understanding of the technical analysisSustainability Standards Modeling Results of Technicalfor the technical analysis of sustainability standards.

  4. Time Series Photometry Data: Standard Access, Standard Formats

    E-Print Network [OSTI]

    Holl, András

    Time Series Photometry Data: Standard Access, Standard Formats Andr#19; as Holl Konkoly Observatory a discussion on data access and #12;le format aspects of photometry. Introduction Presently there is time series photometry data available in public databases, but the access to these varies from one collection

  5. Standard and non standard ideas for the Higgs boson sector

    E-Print Network [OSTI]

    Abbondandolo, Alberto

    Standard and non standard ideas for the Higgs boson sector Riccardo Barbieri Johns Hopkins Workshop as the Higgs boson is in their low-energy spectrum We only know of approximate symmetries that can explain synoptic table Supersymmetric The SM Higgs boson TC-like Minimally extended Higgsless "Composite" 5

  6. Standard-E hydrogen monitoring system shop acceptance test procedure

    SciTech Connect (OSTI)

    Schneider, T.C.

    1997-10-02

    The purpose of this report is to document that the Standard-E Hydrogen Monitoring Systems (SHMS-E), fabricated by Mid-Columbia Engineering (MCE) for installation on the Waste Tank Farms in the Hanford 200 Areas, are constructed as intended by the design. The ATP performance will verify proper system fabrication.

  7. Feasibility of VHE gamma ray detection by an array of imaging atmospheric Cherenkov telescopes using the fluorescence technique

    E-Print Network [OSTI]

    Contreras, J L; Arqueros, F; López, M; Barrio, J A; Nievas, M

    2015-01-01

    The last 20 years have seen the development of new techniques in Astroparticle Physics providing access to the highest end of the electromagnetic spectrum. It has been shown that some sources emit photons up to energies close to 100 TeV. Yet the fluxes of these photons are incredibly low and new detection techniques are needed to go higher in energy. A new technique that would use the new generation of Cherenkov Telescopes, i.e., the Cherenkov Telescope Array (CTA), is proposed to push further the energy frontier. It is based on the detection of the fluorescence radiation emitted in extensive air showers, a successful method used in ultra-high-energy cosmic ray experiments, like the Pierre Auger Observatory. It would complement the standard imaging atmospheric Cherenkov technique with only minor modifications of the hardware currently being developed for the CTA and would not imply significant extra costs during its planned operation.

  8. Cerenkov emission induced by external beam radiation stimulates molecular fluorescence

    SciTech Connect (OSTI)

    Axelsson, Johan; Davis, Scott C.; Gladstone, David J.; Pogue, Brian W.

    2011-07-15

    Purpose: Cerenkov emission is induced when a charged particle moves faster than the speed of light in a given medium. Both x-ray photons and electrons produce optical Cerenkov photons in everyday radiation therapy of tissue; yet, this phenomenon has never been fully documented. This study quantifies the emissions and also demonstrates that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms. Methods: In this study, Cerenkov emission induced by radiation from a clinical linear accelerator is investigated. Biological mimicking phantoms were irradiated with x-ray photons, with energies of 6 or 18 MV, or electrons at energies 6, 9, 12, 15, or 18 MeV. The Cerenkov emission and the induced molecular fluorescence were detected by a camera or a spectrometer equipped with a fiber optic cable. Results: It is shown that both x-ray photons and electrons, at MeV energies, produce optical Cerenkov photons in tissue mimicking media. Furthermore, we demonstrate that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms. Conclusions: The results here indicate that molecular fluorescence monitoring during external beam radiotherapy is possible.

  9. Protein- protein interaction detection system using fluorescent protein microdomains

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2010-02-23

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  10. Red phosphors for use in high CRI fluorescent lamps

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

    2005-11-15

    Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

  11. Federal Information Processing Standards Publication 197

    E-Print Network [OSTI]

    controls. Exports of cryptographic modules implementing this standard and technical data regarding themFederal Information Processing Standards Publication 197 November 26, 2001 Announcing the ADVANCED ENCRYPTION STANDARD (AES) Federal Information Processing Standards Publications (FIPS PUBS) are issued

  12. Appliance Standards and Rulemaking Federal Advisory Committee...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Issuance Appliance Standards and Rulemaking Federal Advisory Committee (ASRAC) - Central Air Conditioner Regional Standards Enforcement Working Group; Notice of Open...

  13. How to align field guides and standards to the Standard Work Specifications

    Broader source: Energy.gov [DOE]

    This presentation demonstrates how to align field guides and standards to the Standard Work Specifications.

  14. Micro-Raman Spectroscopy of Algae: Composition Analysis and Fluorescence Background Behavior

    E-Print Network [OSTI]

    ARTICLE Micro-Raman Spectroscopy of Algae: Composition Analysis and Fluorescence Background performed using Stokes Raman scattering for compositional analysis of algae. Two algal species, Chlorella while acquiring Raman signals from the algae. The time dependence of fluorescence background is char

  15. Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit

    E-Print Network [OSTI]

    Agmon, Noam

    Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit protein, photo-excitation leads to excited-state proton transfer from its chromophore, leaving behind a strongly fluorescing anion, while the proton is commonly thought to migrate internally to Glu-222. X

  16. Fluorescent-Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle

    E-Print Network [OSTI]

    Campbell, Robert E.

    Fluorescent-Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle Robert E. Campbell University of Alberta Genetically-encoded biosensors based on FRET between fluorescent proteins.acs.org/journal/ancham.) The definition of a "biosensor"sthat is, a detection system that relies on a biomolecule for molecular

  17. Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy

    E-Print Network [OSTI]

    Huang, Yanyi

    Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy non-fluorescent nanodiamonds in living cells using transient absorption microscopy. This label of nanodiamonds under various conditions, confirming the endocytosis mechanism. Optical probes for live cell

  18. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  19. Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein John T. M. Kennis,*,

    E-Print Network [OSTI]

    van Stokkum, Ivo

    States *S Supporting Information ABSTRACT: Cyan, green, yellow, and red fluorescent proteins (FPs) homologous to green fluorescent protein (GFP) are used extensively as model systems to study fundamental processes in photobiology, such as the capture of light energy by protein-embedded chromophores, color

  20. Carbon dioxide UV laser-induced fluorescence in high-pressure flames

    E-Print Network [OSTI]

    Lee, Tonghun

    Carbon dioxide UV laser-induced fluorescence in high-pressure flames W.G. Bessler a , C. Schulz a; in final form 16 May 2003 Published online 10 June 2003 Abstract Laser-induced fluorescence (LIF) of carbon dioxide is investigated with excitation between 215 and 255 nm with spectrally resolved detection in 5

  1. End-to-end absolute energy calibration of atmospheric fluorescence telescopes by an electron linear accelerator

    E-Print Network [OSTI]

    End-to-end absolute energy calibration of atmospheric fluorescence telescopes by an electron linear of fluorescence telescopes by using air showers induced by electron beams from a linear accelerator, which and constructing a compact linear accelerator with a maximum electron energy of 40 MeV and an intensity of 6.4 m

  2. Tuning the Fluorescence Emission Spectra of a Single Molecule with a Variable Optical Subwavelength Metal Microcavity

    E-Print Network [OSTI]

    Enderlein, Jörg

    Tuning the Fluorescence Emission Spectra of a Single Molecule with a Variable Optical Subwavelength on changing the fluorescence emission spectrum of a single molecule by embedding it within a tunable planar and coupling are both frequency dependent, this leads to a renormalization of the emission spectrum

  3. Rapid effects of diverse toxic water pollutants on chlorophyll a fluorescence: Variable responses

    E-Print Network [OSTI]

    Berges, John A.

    among freshwater microalgae Chang Jae Choi 1 , John. A. Berges, Erica. B. Young* Department a fluorescence of microalgae is a compelling indicator of toxicity of dissolved water contaminants, because on chlorophyll a fluorescence: Variable responses among freshwater microalgae, Water Research (2012), doi:10

  4. A FRET-Facilitated Photoswitching Using an Orange Fluorescent Protein with the Fast Photoconversion Kinetics

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    resonance energy transfer (FRET) from green fluorescent donors. We have termed the latter effect a FRET available photoconvertible green- to-red probes are in high demand. We have developed a monomericEos2,4 Kaede,5 mKikGR,6 mClavGR27 and their derivatives, change their fluorescent color from green

  5. Fully adaptive FEM based fluorescence optical tomography from time-dependent measurements with area illumination

    E-Print Network [OSTI]

    Bangerth, Wolfgang

    Fully adaptive FEM based fluorescence optical tomography from time-dependent measurements with area fluorescence absorption map with an adaptive finite element based scheme. The tissue phantom consisted 785 nm diode laser light and a gain modulated image intensified charge coupled device camera

  6. Spectroscopic Properties of a Self-Assembled Zinc Porphyrin Tetramer II. Time-Resolved Fluorescence Spectroscopy

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Spectroscopic Properties of a Self-Assembled Zinc Porphyrin Tetramer II. Time-Resolved Fluorescence tetramer [Part I], with a 1 ns rotational correlation time at 10 °C. The initial fluorescence anisotropy of the monomer is found to be 0.1. In the tetramer an additional depolarization process occurs with a correlation

  7. Plasmon-controlled fluorescence: A new detection technology Joseph R. Lakowicz*a

    E-Print Network [OSTI]

    Turro, Nicholas J.

    Plasmon-controlled fluorescence: A new detection technology Joseph R. Lakowicz*a , Mustafa H fundamentally change and increase the capabilities of fluorescence technology. This change will be based to control the decay rates, location and direction of fluorophore emission. We refer to this technology

  8. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM)

    2007-09-18

    The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

  9. In Vitro Transport of a Fluorescent Nuclear Protein and Exclusion of Non-Nuclear Proteins

    E-Print Network [OSTI]

    Forbes, Douglass

    In Vitro Transport of a Fluorescent Nuclear Protein and Exclusion of Non-Nuclear Proteins Donald D microscopic assay for nuclear transport. The assay uses an extract of Xenopus eggs, normal or synthetic nuclei, and a fluorescently labeled nuclear protein, nucleoplasmin. This in vitro system accurately mimics in vivo nuclear

  10. Classification with Artificial Neural Networks and Support Vector Machines: application to oil fluorescence spectra

    E-Print Network [OSTI]

    Oldenburg, Carl von Ossietzky Universität

    be applied to predict the profit, market movements, and price level based on the market's historical datasetClassification with Artificial Neural Networks and Support Vector Machines: application to oil, and Oil fluorescence ABSTRACT: This paper reports on oil classification with fluorescence spectroscopy

  11. Reverse engineering the ancient ceramic technology based on X-ray fluorescence spectromicroscopy

    E-Print Network [OSTI]

    Reverse engineering the ancient ceramic technology based on X-ray fluorescence spectromicroscopy-ray fluorescence (XRF) microprobe analyses of ancient ceramic cross-sections aiming at deciphering the different and Fe K-edge X-ray absorption near edge structure spectroscopy were performed on pre-sigillata ceramics

  12. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2011-06-07

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  13. Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers

    DOE Patents [OSTI]

    Walsh, Joseph C.; Heiss, Aaron M.; Noronha, Glenn; Vachon, David J.; Lane, Stephen M.; Satcher, Jr., Joe H.; Peyser, Thomas A.; Van Antwerp, William Peter; Mastrototaro, John Joseph

    2004-07-20

    Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

  14. Hyperspectral image reconstruction for x-ray fluorescence tomography

    SciTech Connect (OSTI)

    Gürsoy, Do?a; Biçer, Tekin; Lanzirotti, Antonio; Newville, Matthew G.; De Carlo, Francesco

    2015-01-01

    A penalized maximum-likelihood estimation is proposed to perform hyperspectral (spatio-spectral) image reconstruction for X-ray fluorescence tomography. The approach minimizes a Poisson-based negative log-likelihood of the observed photon counts, and uses a penalty term that has the effect of encouraging local continuity of model parameter estimates in both spatial and spectral dimensions simultaneously. The performance of the reconstruction method is demonstrated with experimental data acquired from a seed of arabidopsis thaliana collected at the 13-ID-E microprobe beamline at the Advanced Photon Source. The resulting element distribution estimates with the proposed approach show significantly better reconstruction quality than the conventional analytical inversion approaches, and allows for a high data compression factor which can reduce data acquisition times remarkably. In particular, this technique provides the capability to tomographically reconstruct full energy dispersive spectra without compromising reconstruction artifacts that impact the interpretation of results.

  15. Semiconductor Quantum Rods as Single Molecule FluorescentBiological Labels

    SciTech Connect (OSTI)

    Fu, Aihua; Gu, Weiwei; Boussert, Benjamine; Koski, Kristie; Gerion, Daniele; Manna, Liberato; Le Gros, Mark; Larabell, Carolyn; Alivisatos, A. Paul

    2006-05-29

    In recent years, semiconductor quantum dots have beenapplied with great advantage in a wide range of biological imagingapplications. The continuing developments in the synthesis of nanoscalematerials and specifically in the area of colloidal semiconductornanocrystals have created an opportunity to generate a next generation ofbiological labels with complementary or in some cases enhanced propertiescompared to colloidal quantum dots. In this paper, we report thedevelopment of rod shaped semiconductor nanocrystals (quantum rods) asnew fluorescent biological labels. We have engineered biocompatiblequantum rods by surface silanization and have applied them fornon-specific cell tracking as well as specific cellular targeting. Theproperties of quantum rods as demonstrated here are enhanced sensitivityand greater resistance for degradation as compared to quantum dots.Quantum rods have many potential applications as biological labels insituations where their properties offer advantages over quantumdots.

  16. Hyperspectral image reconstruction for x-ray fluorescence tomography

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gürsoy, Do?a; Biçer, Tekin; Lanzirotti, Antonio; Newville, Matthew G.; De Carlo, Francesco

    2015-01-01

    A penalized maximum-likelihood estimation is proposed to perform hyperspectral (spatio-spectral) image reconstruction for X-ray fluorescence tomography. The approach minimizes a Poisson-based negative log-likelihood of the observed photon counts, and uses a penalty term that has the effect of encouraging local continuity of model parameter estimates in both spatial and spectral dimensions simultaneously. The performance of the reconstruction method is demonstrated with experimental data acquired from a seed of arabidopsis thaliana collected at the 13-ID-E microprobe beamline at the Advanced Photon Source. The resulting element distribution estimates with the proposed approach show significantly better reconstruction quality than the conventional analytical inversionmore »approaches, and allows for a high data compression factor which can reduce data acquisition times remarkably. In particular, this technique provides the capability to tomographically reconstruct full energy dispersive spectra without compromising reconstruction artifacts that impact the interpretation of results.« less

  17. Fluorescence Correlation Spectroscopy at Micromolar Concentrations without Optical Nanoconfinement

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Laurence, Ted A.; Ly, Sonny; Bourguet, Feliza; Fischer, Nicholas O.; Coleman, Matthew A.

    2014-08-14

    Fluorescence correlation spectroscopy (FCS) is an important technique for studying biochemical interactions dynamically that may be used in vitro and in cell-based studies. It is generally claimed that FCS may only be used at nM concentrations. We show that this general consensus is incorrect and that the limitation to nM concentrations is not fundamental but due to detector limits as well as laser fluctuations. With a high count rate detector system and applying laser fluctuation corrections, we demonstrate FCS measurements up to 38 ?M with the same signal-to-noise as at lower concentrations. Optical nanoconfinement approaches previously used to increase themore »concentration range of FCS are not necessary, and further increases above 38 ?M may be expected using detectors and detector arrays with higher saturation rates and better laser fluctuation corrections. This approach greatly widens the possibilities of dynamic measurements of biochemical interactions using FCS at physiological concentrations.« less

  18. Fluorescence Correlation Spectroscopy at Micromolar Concentrations without Optical Nanoconfinement

    SciTech Connect (OSTI)

    Laurence, Ted A.; Ly, Sonny; Bourguet, Feliza; Fischer, Nicholas O.; Coleman, Matthew A.

    2014-08-14

    Fluorescence correlation spectroscopy (FCS) is an important technique for studying biochemical interactions dynamically that may be used in vitro and in cell-based studies. It is generally claimed that FCS may only be used at nM concentrations. We show that this general consensus is incorrect and that the limitation to nM concentrations is not fundamental but due to detector limits as well as laser fluctuations. With a high count rate detector system and applying laser fluctuation corrections, we demonstrate FCS measurements up to 38 ?M with the same signal-to-noise as at lower concentrations. Optical nanoconfinement approaches previously used to increase the concentration range of FCS are not necessary, and further increases above 38 ?M may be expected using detectors and detector arrays with higher saturation rates and better laser fluctuation corrections. This approach greatly widens the possibilities of dynamic measurements of biochemical interactions using FCS at physiological concentrations.

  19. National Green Building Standard Analysis

    SciTech Connect (OSTI)

    none,

    2012-07-01

    DOE's Building America Program is a research and development program to improve the energy performance of new and existing homes. The ultimate goal of the Building America Program is to achieve examples of cost-effective, energy efficient solutions for all U.S. climate zones. Periodic maintenance of an ANSI standard by review of the entire document and action to revise or reaffirm it on a schedule not to exceed five years is required by ANSI. In compliance, a consensus group has once again been formed and the National Green Building Standard is currently being reviewed to comply with the periodic maintenance requirement of an ANSI standard.

  20. Functional design criteria for standard hydrogen monitoring system portable platform

    SciTech Connect (OSTI)

    Schneider, T.C.

    1997-01-17

    Functional design description for a Standard-E cabinet arrangement Standard Hydrogen Monitoring System Portable Platform.

  1. Commercial Building Codes and Standards

    Broader source: Energy.gov [DOE]

    Once an energy-efficient technology or practice is widely available in the market, it can become the baseline of performance through building energy codes and equipment standards. The Building...

  2. Cyanine-based probe\\tag-peptide pair for fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana (Richland, WA); Cao, Haishi (Richland, WA)

    2010-08-17

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  3. Using co-cultures expressing fluorescence resonance energy transfer based protein biosensors to simultaneously image caspase-3 and Ca2+

    E-Print Network [OSTI]

    Using co-cultures expressing fluorescence resonance energy transfer based protein biosensors received: 8 June 2005; Accepted 11 June 2005 Key words: biosensor, fluorescence imaging, fluorescence)-based protein biosensors allow the spatial and temporal imaging of signaling events in living cells. However

  4. Technical Standards Newsletter - May 2001 | Department of Energy

    Energy Savers [EERE]

    Technical Standards Newsletter - May 2001 Technical Standards Newsletter - May 2001 The Standards Forum and Standards Actions - May 2001 INSIDE THIS ISSUE: DOE Technical Standards...

  5. Diagnostic Medical Sonography Clinical Technical Standards

    E-Print Network [OSTI]

    Barrash, Warren

    Diagnostic Medical Sonography Clinical Technical Standards Listed below are the technical standards identified for students in the Diagnostic Medical Sonography (DMS) clinical program. Review each standard and think about your ability to meet the standard 100% or if you are unable to meet the standard, what

  6. THE SAP3 COMPUTER PROGRAM FOR QUANTITATIVE MULTIELEMENT ANALYSIS BY ENERGY DISPERSIVE X-RAY FLUORESCENCE

    SciTech Connect (OSTI)

    Nielson, K. K.; Sanders, R. W.

    1982-04-01

    SAP3 is a dual-function FORTRAN computer program which performs peak analysis of energy-dispersive x-ray fluorescence spectra and then quantitatively interprets the results of the multielement analysis. It was written for mono- or bi-chromatic excitation as from an isotopic or secondary excitation source, and uses the separate incoherent and coherent backscatter intensities to define the bulk sample matrix composition. This composition is used in performing fundamental-parameter matrix corrections for self-absorption, enhancement, and particle-size effects, obviating the need for specific calibrations for a given sample matrix. The generalized calibration is based on a set of thin-film sensitivities, which are stored in a library disk file and used for all sample matrices and thicknesses. Peak overlap factors are also determined from the thin-film standards, and are stored in the library for calculating peak overlap corrections. A detailed description is given of the algorithms and program logic, and the program listing and flow charts are also provided. An auxiliary program, SPCAL, is also given for use in calibrating the backscatter intensities. SAP3 provides numerous analysis options via seventeen control switches which give flexibility in performing the calculations best suited to the sample and the user needs. User input may be limited to the name of the library, the analysis livetime, and the spectrum filename and location. Output includes all peak analysis information, matrix correction factors, and element concentrations, uncertainties and detection limits. Twenty-four elements are typically determined from a 1024-channel spectrum in one-to-two minutes using a PDP-11/34 computer operating under RSX-11M.

  7. Nuclear Data Verification and Standardization

    SciTech Connect (OSTI)

    Karam, Lisa R.; Arif, Muhammad; Thompson, Alan K.

    2011-10-01

    The objective of this interagency program is to provide accurate neutron interaction verification and standardization data for the U.S. Department of Energy Division of Nuclear Physics programs which include astrophysics, radioactive beam studies, and heavy-ion reactions. The measurements made in this program are also useful to other programs that indirectly use the unique properties of the neutron for diagnostic and analytical purposes. These include homeland security, personnel health and safety, nuclear waste disposal, treaty verification, national defense, and nuclear based energy production. The work includes the verification of reference standard cross sections and related neutron data employing the unique facilities and capabilities at NIST and other laboratories as required; leadership and participation in international intercomparisons and collaborations; and the preservation of standard reference deposits. An essential element of the program is critical evaluation of neutron interaction data standards including international coordinations. Data testing of critical data for important applications is included. The program is jointly supported by the Department of Energy and the National Institute of Standards and Technology.

  8. Improved model for the analysis of air fluorescence induced by electrons

    E-Print Network [OSTI]

    F. Arqueros; F. Blanco; J. Rosado

    2008-07-24

    A model recently proposed for the calculation of air-fluorescence yield excited by electrons is revisited. Improved energy distributions of secondary electrons and a more realistic Monte Carlo simulation including some additional processes have allowed us to obtain more accurate results. The model is used to study in detail the relationship between fluorescence intensity and deposited energy in a wide range of primary energy (keVs - GeVs). In addition, predictions on the absolute value of the fluorescence efficiency in the absence of collisional quenching will be presented and compared with available experimental data.

  9. George Mason University Architecture Standards Committee Charter

    E-Print Network [OSTI]

    and maintain technology architecture standards; · Establish, publish and maintain software development, testing bodies; · Assess periodically technology architecture standards; · Assess periodically the proceduresGeorge Mason University Architecture Standards Committee Charter Page 1 of 3 12/2/2013 Purpose

  10. An overview of software safety standards

    SciTech Connect (OSTI)

    Lawrence, J.D.

    1995-10-01

    The writing of standards for software safety is an increasingly important activity. This essay briefly describes the two primary standards-writing organizations, IEEE and IEC, and provides a discussion of some of the more interesting software safety standards.

  11. Information Technology: American National Standard for Information

    E-Print Network [OSTI]

    Information Technology: American National Standard for Information Systems-- Data Format-271 Information Technology: American National Standard for Information Systems-- Data Format for the Interchange Sponsored by Information Access Division Information Technology Laboratory National Institute of Standards

  12. An Estoppel Doctrine for Patented Standards

    E-Print Network [OSTI]

    Merges, Robert P; Kuhn, Jeffrey

    2008-01-01

    to defend suit over industry standards, but the patenteesThrough the Capture of Industry Standards, 17 B ERKELEY Tin the face of an industry standard that infringes a patent

  13. The standard model and colliders

    SciTech Connect (OSTI)

    Hinchliffe, I.

    1987-03-01

    Some topics in the standard model of strong and electroweak interactions are discussed, as well as how these topics are relevant for the high energy colliders which will become operational in the next few years. The radiative corrections in the Glashow-Weinberg-Salam model are discussed, stressing how these corrections may be measured at LEP and the SLC. CP violation is discussed briefly, followed by a discussion of the Higgs boson and the searches which are relevant to hadron colliders are then discussed. Some of the problems which the standard model does not solve are discussed, and the energy ranges accessible to the new colliders are indicated. (LEW)

  14. Continuous Flow Cryostat for X-Ray Fluorescence

    SciTech Connect (OSTI)

    Weng, T.-C.; Linden, Peter J. E. M. van der; Glatzel, Pieter; Lapras, Christophe; Krzyzowski, Michael

    2010-06-23

    A continuous Helium flow cryostat was designed and built by Cryovac GMbH to specifications given by ESRF beamline ID26. The beamline has constructed a high energy resolution X-ray emission spectrometer using multiple spherically bent analyser crystals, together with the sample and detector on a vertical Rowland circle. The double shrouded cryostat has a low profile designed to fit into the spectrometer setup, the lowest detector position allows for a Bragg angle of 85 degrees with a 1 meter diameter Rowland circle. The cryostat has a temperature range of 5 to 300 Kelvin on the sample holder which is cooled by static Helium exchange gas. The cryostat has triple windows for beam entrance, transmission and fluorescence; the latter offers an opening angle of 80 degrees horizontally and 50 degrees vertically. The cryostat can be configured to work in two different operation modes: translation or rotation. The translation mode offers a displacement of 50 mm to accommodate multiple samples on the sample holder. The rotation mode is used for polarisation studies on single crystals.We show recent results obtained on Chromium containing molecular complexes; data collection was done at a temperature of 10 Kelvin to avoid radiation damage.

  15. Fluorescence from a quantum dot and metallic nanosphere hybrid system

    SciTech Connect (OSTI)

    Schindel, Daniel G. [Department of Mathematics and Statistics, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB, R3B 2E9 (Canada); Singh, Mahi R. [Department of Physics and Astronomy, University of Western Ontario, 1151 Richmond Street, London, ON, N6A 3K7 (Canada)

    2014-03-31

    We present energy absorption and interference in a quantum dot-metallic nanosphere system embedded on a dielectric substrate. A control field is applied to induce dipole moments in the nanosphere and the quantum dot, and a probe field is applied to monitor absorption. Dipole moments in the quantum dot or the metal nanosphere are induced, both by the external fields and by each other's dipole fields. Thus, in addition to direct polarization, the metal nanosphere and the quantum dot will sense one another via the dipole-dipole interaction. The density matrix method was used to show that the absorption spectrum can be split from one peak to two peaks by the control field, and this can also be done by placing the metal sphere close to the quantum dot. When the two are extremely close together, a self-interaction in the quantum dot produces an asymmetry in the absorption peaks. In addition, the fluorescence efficiency can be quenched by the addition of a metal nanosphere. This hybrid system could be used to create ultra-fast switching and sensing nanodevices.

  16. Technical Standards, Program Project Justification Statement...

    Broader source: Energy.gov (indexed) [DOE]

    August 29, 2012 DOE Technical Standards Program Project Justification Statement As part of the new justification process for developing or revising DOE Technical Standards,...

  17. Quality Assurance Functional Area Qualification Standard - DOE...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    nuclear facilities. DOE-STD-1150-2013, Quality Assurance Functional Area Qualification Standard Type: Invoked Technical Standards Status: Current Approved Date: Dec 02, 2013...

  18. Developing Integrated National Design Standards for Offshore...

    Energy Savers [EERE]

    Developing Integrated National Design Standards for Offshore Wind Plants Developing Integrated National Design Standards for Offshore Wind Plants January 6, 2014 - 10:00am Addthis...

  19. Standardization of Transport Properties Measurements: Internal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Standardization of Transport Properties Measurements: Internal Energy Agency (IEA-AMT) Annex on Thermoelectric Standardization of Transport Properties Measurements: Internal Energy...

  20. Enforcement Policy Statement: Regional Standards Enforcement...

    Office of Environmental Management (EM)

    set forth amended energy conservation standards for central air conditioners and heat pumps, including regional standards in certain States. 76 FR 37408. DOE has initiated a...

  1. Cooperation on Sustainability Standards | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cooperation on Sustainability Standards Cooperation on Sustainability Standards Keith Kline, ORNL, presentation at the December 5, 2012, Biomass Program-hosted International...

  2. Standards Increase Market Confidence in SSL Performance

    SciTech Connect (OSTI)

    2013-09-30

    Fact sheet that reviews current and future SSL standards developed by DOE and other standards-setting organizations to effectively measure and characterize SSL lighting products.

  3. LED-Induced Fluorescence System for Tea Classification and Quality Assessment

    E-Print Network [OSTI]

    Dong, Yongjiang; Mei, Liang; Feng, Chao; Yan, Chunsheng; He, Sailing

    2013-01-01

    A fluorescence system is developed by using several light emitting diodes (LEDs) with different wavelengths as excitation light sources. The fluorescence detection head consists of multi LED light sources and a multimode fiber for fluorescence collection, where the LEDs and the corresponding filters can be easily chosen to get appropriate excitation wavelengths for different applications. By analyzing fluorescence spectra with the principal component analysis method, the system is utilized in the classification of four types of green tea beverages and two types of black tea beverages. Qualities of the Xihu Longjing tea leaves of different grades, as well as the corresponding liquid tea samples, are studied to further investigate the ability and application of the system in the evaluation of classification/quality of tea and other foods.

  4. Antibody-Antigen assay design for combined optical tweezers and fluorescence

    E-Print Network [OSTI]

    Ta, Jenny, 1982-

    2004-01-01

    The recent development in combined optical trapping and fluorescence technology promises to enable unbindinig force studies of receptor-ligand interactions, whose specificity play a crucial role in the function of many ...

  5. A detection instrument for enhanced-fluorescence and label-free imaging on photonic

    E-Print Network [OSTI]

    Cunningham, Brian

    A detection instrument for enhanced- fluorescence and label-free imaging on photonic crystal detection of dye-tagged hybridized DNA yields 3x more genes to be detected versus commercially available

  6. Genetically encoded biosensors based on engineered fluorescent proteins Wolf B. Frommer, Michael W. Davidson, Robert Campbell

    E-Print Network [OSTI]

    Campbell, Robert E.

    Genetically encoded biosensors based on engineered fluorescent proteins Wolf B. Frommer, Michael W. Davidson, Robert Campbell Supplementary Table I. List of biosensors based on single polypeptide chains Biosensor specificity Representative biosensor(s) Reference(s) Translocation-based biosensors

  7. Metal-based turn-on fluorescent probes for nitric oxide sensing

    E-Print Network [OSTI]

    Lim, Mi Hee

    2006-01-01

    Chapter 1. Metal-Based Turn-On Fluorescent Probes for Sensing Nitric Oxide. Nitric oxide, a reactive free radical, regulates a variety of biological processes. The absence of tools to detect NO directly, rapidly, specifically ...

  8. Turn-on fluorescent probes for detecting nitric oxide in biology

    E-Print Network [OSTI]

    McQuade, Lindsey Elizabeth, 1981-

    2010-01-01

    Chapter 1. Investigating the Biological Roles of Nitric Oxide and Other Reactive Nitrogen Species Using Fluorescent Probes: This chapter presents an overview of recent progress in the field of reactive nitrogen species ...

  9. Organelle-selective energy transfer: A fluorescent indicator of intracellular environment

    E-Print Network [OSTI]

    Burgess, Kevin

    Organelle-selective energy transfer: A fluorescent indicator of intracellular environment Aurore emission wavelength of a dye changes significantly with its intra- cellular environment. Probes monitoring dynamic organelle specific functions. Our group has an ongoing interest in `energy transfer

  10. Examining 239Pu and 240Pu Nuclear Resonance Fluorescence Measurements on Spent Fuel for Nuclear Safeguards

    E-Print Network [OSTI]

    Quiter, Brian

    2013-01-01

    10- 01096) Journal of Nuclear Technology, p. 150, Vol. 175,linac and laser technologies for nuclear photonics gamma-rayNuclear resonance fluorescence (NRF) has been identified as a technology

  11. Fast Flow Microfluidics and Single-Molecule Fluorescence for the Rapid Characterization of ?-Synuclein Oligomers

    E-Print Network [OSTI]

    Horrocks, Mathew H.; Tosatto, Laura; Dear, Alexander J.; Garcia, Gonzalo A.; Iljina, Marija; Cremades, Nunilo; Serra, Mauro Dalla; Knowles, Tuomas P. J.; Dobson, Christopher M.; Klenerman, David

    2015-08-10

    traditional biochemical techniques. By combining fast-flow microfluidics with single-molecule fluorescence, we are able to rapidly follow the process by which oligomers of ?S are formed and to characterize the species themselves. We have used the technique...

  12. Time resolved fluorescence of CdSe nanocrystals using single molecule spectroscopy

    E-Print Network [OSTI]

    Fisher, Brent R

    2005-01-01

    A wide variety of spectroscopic studies of CdSe nanocrystals (NCs) are presented in this thesis, all studying some aspect of the temporal evolution of NC fluorescence tinder different conditions. In particular the methods ...

  13. Experimental Fluorescence Optical Tomography using Adaptive Finite Elements and Planar Illumination with

    E-Print Network [OSTI]

    Bangerth, Wolfgang

    Experimental Fluorescence Optical Tomography using Adaptive Finite Elements and Planar Illumination with Modulated Excitation Light Amit Joshia , Wolfgang Bangerthb , Alan B. Thompsona , and Eva M. Sevick adaptive discretization strategies. To date, the efficacy of adaptive mesh refinement techniques has yet

  14. Measurement and model assessment of fluorescence lifetime sensing in multiply scattering media 

    E-Print Network [OSTI]

    Kuwana, Eddy

    2005-08-29

    implant, the fluorescent decay kinetics can be sensitive to the tissue??s biochemical environment, providing quantitative in vivo information of the confined tissue site. The impact of light propagation and decay kinetics upon the measured signals...

  15. Simulations of time-dependent fluorescence in nano-confined solvents

    E-Print Network [OSTI]

    Thompson, Ward H.

    2004-04-13

    The time-dependent fluorescence of a model diatomic molecule with a charge-transfer electronic transition in confined solvents has been simulated. The effect of confining the solvent is examined by comparing results for solutions contained within...

  16. Nuclear Resonance Fluorescence to Measure Plutonium Mass in Spent Nuclear Fuel

    E-Print Network [OSTI]

    Ludewigt, Bernhard A

    2011-01-01

    and S.J. Thompson,“Determining Plutonium in Spent Fuel withTobin, “Determination of Plutonium Content in Spent FuelFluorescence to Measure Plutonium Mass in Spent Nuclear Fuel

  17. An improved understanding of fluorescent Zn(II) sensors and their uses in biological settings

    E-Print Network [OSTI]

    Wong, Brian Alexander

    2009-01-01

    Chapter 1. An Introduction to Fluorescent Zn(II) Sensors and Their Applications in Biological Systems This chapter opens with an overview of the numerous roles of zinc in biology, with an emphasis on labile Zn(II), that ...

  18. The Application of Fluorescence Lifetime Imaging Microscopy to Quantitatively Map Mixing and Temperature in Microfluidic Systems 

    E-Print Network [OSTI]

    Graham, Emmelyn M

    2008-01-01

    The technique of Fluorescence Lifetime Imaging Microscopy (FLIM) has been employed to quantitatively and spatially map the fluid composition and temperature within microfluidic systems. A molecular probe with a ...

  19. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons 

    E-Print Network [OSTI]

    Zhao, Suling; Maxwell, Sarah L; Jimenez-Beristain, Antonio; Vives, Joaquim; Kuehner, Eva; Zhao, Jiexin; O'Brian, Carmel; de Felipe, Carmen; Semina, Elena

    2004-01-01

    We have generated embryonic stem cells and transgenic mice with green fluorescent protein (GFP) inserted into the Pitx3 locus via homologous recombination. In the central nervous system, Pitx3 directed GFP was visualised ...

  20. Development and analysis of recombinant fluorescent probes for use in live cell imaging of filamentous fungi 

    E-Print Network [OSTI]

    Altenbach, Kirsten

    2010-01-01

    The molecular cloning and subsequent engineering of the green fluorescent protein (GFP) of the jellyfish Aequoria victoria allowed a novel approach to the investigation of cell signalling. GFP and its mutants can now not ...

  1. Automation of the Laguerre Expansion Technique for Analysis of Time-resolved Fluorescence Spectroscopy Data 

    E-Print Network [OSTI]

    Dabir, Aditi Sandeep

    2010-07-14

    Time-resolved fluorescence spectroscopy (TRFS) is a powerful analytical tool for quantifying the biochemical composition of organic and inorganic materials. The potentials of TRFS as nondestructive clinical tool for tissue diagnosis have been...

  2. Development of a Time Resolved Fluorescence Spectroscopy System for Near Real-Time Clinical Diagnostic Applications 

    E-Print Network [OSTI]

    Trivedi, Chintan A.

    2010-07-14

    The design and development of a versatile time resolved fluorescence spectroscopy (TRFS) system capable of near real time data acquisition and processing for potential clinical diagnostic applications is reported. The TRFS apparatus is portable...

  3. Furniture Standards Contract Price Guide

    E-Print Network [OSTI]

    Sussex, University of

    1 Furniture Standards Contract Price Guide Issue 1 - July 2007 www.broadstock.co.uk University of Sussex #12;2 Contents Office 5 1600 x 1600 Universal workstations 6 Double wave desks 7 Fixed tables 7 Circular tables 7 Bench 8 Screens 10 Electrics 12 Storage 13 Pedestals 14 Magnus wooden storage 15 Metrix

  4. Residential ventilation standards scoping study

    SciTech Connect (OSTI)

    McKone, Thomas E.; Sherman, Max H.

    2003-10-01

    The goals of this scoping study are to identify research needed to develop improved ventilation standards for California's Title 24 Building Energy Efficiency Standards. The 2008 Title 24 Standards are the primary target for the outcome of this research, but this scoping study is not limited to that timeframe. We prepared this scoping study to provide the California Energy Commission with broad and flexible options for developing a research plan to advance the standards. This document presents the findings of a scoping study commissioned by the Public Interest Energy Research (PIER) program of the California Energy Commission to determine what research is necessary to develop new residential ventilation requirements for California. This study is one of three companion efforts needed to complete the job of determining the ventilation needs of California residences, determining the bases for setting residential ventilation requirements, and determining appropriate ventilation technologies to meet these needs and requirements in an energy efficient manner. Rather than providing research results, this scoping study identifies important research questions along with the level of effort necessary to address these questions and the costs, risks, and benefits of pursuing alternative research questions. In approaching these questions and corresponding levels of effort, feasibility and timing were important considerations. The Commission has specified Summer 2005 as the latest date for completing this research in time to update the 2008 version of California's Energy Code (Title 24).

  5. Nanoscale Stress Measurements and Standards

    E-Print Network [OSTI]

    Magee, Joseph W.

    Nanoscale Stress Measurements and Standards SEMICONDUCTORS Our objective is to develop accurate and lifetime, and address a critical measurement need in the MEMS industry, i.e., 90 % of MEMS customers and Customers · The semiconductor microelectronics industry is a $250B worldwide market with 9% cumulative

  6. On-line measurement of lignin in wood pulp by color shift of fluorescence

    DOE Patents [OSTI]

    Jeffers, L.A.; Malito, M.L.

    1996-01-23

    Lignin concentrations from wood pulp samples are measured by applying an excitation light at a selected wavelength to the samples in order to cause the lignin to emit fluorescence. A spectral distribution of the fluorescence emission is then determined. The lignin concentration is then calculated based on the spectral distribution signal. The spectral distribution is quantified by either a wavelength centroid method or a band ratio method. 6 figs.

  7. On-line measurement of lignin in wood pulp by color shift of fluorescence

    DOE Patents [OSTI]

    Jeffers, Larry A. (Washington Township, Stark County, OH); Malito, Michael L. (Liberty Township, Trumbull County, OH)

    1996-01-01

    Lignin concentrations from wood pulp samples are measured by applying an excitation light at a selected wavelength to the samples in order to cause the lignin to emit fluorescence. A spectral distribution of the fluorescence emission is then determined. The lignin concentration is then calculated based on the spectral distribution signal. The spectral distribution is quantified by either a wavelength centroid method or a band ratio method.

  8. Organic light-emitting device with a phosphor-sensitized fluorescent emission layer

    DOE Patents [OSTI]

    Forrest, Stephen (Ann Arbor, MI); Kanno, Hiroshi (Osaka, JP)

    2009-08-25

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters. The emissive region of the devices of the present invention comprise at least one phosphor-sensitized layer which has a combined emission from a phosphorescent emitter and a fluorescent emitter. In preferred embodiments, the invention relates to white-emitting OLEDS (WOLEDs).

  9. Stardust Interstellar Preliminary Examination VI: Quantitative elemental analysis by synchrotron X-ray fluorescence nanoimaging of eight impact features in aerogel

    E-Print Network [OSTI]

    Nittler, Larry R.

    -ray fluorescence nanoimaging of eight impact features in aerogel Alexandre S. SIMIONOVICI1* , Laurence LEMELLE2

  10. Conservation potential of compact fluorescent lamps in India and Brazil

    SciTech Connect (OSTI)

    Gadgil, A.; Martino Jannuzzi, G. de (Lawrence Berkeley Lab., CA (USA); Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia)

    1989-07-01

    We evaluate the conservation potential of compact fluorescent lamps (CFLs) for managing the rapidly increasing electrical energy and peak demand in India and Brazil. Using very conservative assumptions, we find that the cost of conserved energy using 16 W CFLs is 4 and 6 times less than the long range marginal cost of electricity for the two countries. The cost of avoided peak installed capacity is 6 and 9.5 times less than the cost of new installed capacity for India and Brazil. The analysis is undertaken from the three separate perspectives of the national economies, the consumers, and the utilities. We find that because residential electricity is subsidized, the consumers have little or no incentive to purchase and install the CFLs, unless they too are subsidized. However, the benefits of CFL installation to the utility are so large that subsidizing them is a paying proposition for the utility are so large that subsidizing them is a paying proposition for the utility in almost all cases. As an illustration of a gradual introduction strategy for CFLs, we calculate a scenario where national savings of the order of US $1.2 million per day for India and US $2.5 million per day for Brazil are reached in 10 years by a small and gradual transfer of subsidy from residential electricity to CFLs. We then explore the barriers to immediate large scale introduction of these lamps in the two countries. Specific technical and marketing problems are identified and discussed, which would require solution before such an introduction can be attempted. Lastly, we discuss the range of policy instruments, in addition to a subsidy scheme, that can be used for promoting the diffusion of these lamps in the domestic and commercial sector. 47 refs., 15 figs., 2 tabs.

  11. Lumiram Electric: Proposed Penalty (2010-CE-1014)

    Broader source: Energy.gov [DOE]

    DOE alleged in a Notice of Proposed Civil Penalty that Lumiram Electric Corporation failed to certify a variety of incandescent reflector lamps as compliant with the applicable energy conservation standards.

  12. Lumiram Electric: Order (2010-CE-1014)

    Broader source: Energy.gov [DOE]

    DOE issued an Order and entered into a Compromise Agreement with Lumiram Electric Corporation after finding Lumiram Electric had failed to certify that certain models of incandescent reflector lamps comply with the applicable energy conservation standards.

  13. Lighting Choices - White Background | Department of Energy

    Energy Savers [EERE]

    Choices - White Background Image icon All of these lightbulbs-CFLs, LEDs, and energy-saving incandescents-meet the new energy standards that take effect from 2012-2014. More...

  14. Lighting Choices to Save You Money Banner | Department of Energy

    Energy Savers [EERE]

    to Save You Money Banner Lighting Choices to Save You Money Banner All of these lightbulbs-CFLs, LEDs, and energy-saving incandescents-meet the new energy standards that take...

  15. New Lighting Facts Label: Takes the Guess Work Out of Shopping...

    Office of Environmental Management (EM)

    With new lighting standards taking effect this year, now's a great time switch to energy-saving incandescent, CFL, and LED light bulbs, which are available in most hardware and...

  16. Aero-Tech: Proposed Penalty (2010-CE-1012)

    Broader source: Energy.gov [DOE]

    DOE alleged in a Notice of Proposed Civil Penalty that Aero-Tech Light Bulb Co. failed to certify a variety of incandescent reflector lamps as compliant with the applicable energy conservation standards.

  17. DuraLamp USA: Order (2010-CE-0912)

    Broader source: Energy.gov [DOE]

    DOE ordered DuraLamp USA, Inc. to pay a $2,500 civil penalty after finding DuraLamp USA had failed to certify that model PAR 30, an incandescent reflector lamp, complies with the applicable energy conservation standards.

  18. International Standards in Forensic DNA and

    E-Print Network [OSTI]

    documentary (technical) standards physical (measurement) standards Certified reference material to aidInternational Standards in Forensic DNA and Recent Forensic Science Activities in the United States John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant

  19. Title 24, Part 6 2013 Standards

    E-Print Network [OSTI]

    California at Davis, University of

    -rulemaking industry meetings · Workshops on proposed standards · Notice of Proposed Action and "45-day language 24 2013 Standards Convened more than 45 Industry stakeholder groups over the last year to inform themTitle 24, Part 6 2013 Standards 2013 Building Standards Update Mazi Shirakh, PE Martha Brook, PE

  20. Physics Beyond the Standard Model

    E-Print Network [OSTI]

    Howard Baer

    2009-08-19

    I present a brief overview of some exciting possibilities for physics Beyond the Standard Model. I include short discussions of neutrino physics, the strong CP problem and axions, GUTs, large and warped extra dimensions, Little Higgs models and supersymmetry. The chances appear excellent that in the next few years-- as the LHC era gets underway-- data from a bevy of experiments will point the way to a new paradigm for the laws of physics as we know them.

  1. DOE limited standard: Operations assessments

    SciTech Connect (OSTI)

    NONE

    1996-05-01

    Purpose of this standard is to provide DOE Field Element assessors with a guide for conducting operations assessments, and provide DOE Field Element managers with the criteria of the EM Operations Assessment Program. Sections 6.1 to 6.21 provide examples of how to assess specific areas; the general techniques of operations assessments (Section 5) may be applied to other areas of health and safety (e.g. fire protection, criticality safety, quality assurance, occupational safety, etc.).

  2. Saving Energy and Money with Appliance and Equipment Standards...

    Office of Environmental Management (EM)

    clothes washers, commercial refrigeration equipment, walk-in coolers and freezers, electric motors, general service fluorescent lamps (GSFLs), metal halide lamp fixtures,...

  3. Department Sets Aggressive Schedule for New Appliance Standards...

    Broader source: Energy.gov (indexed) [DOE]

    service lamps; fluorescent lamp ballasts; residential dishwashers; ranges and ovens; microwave ovens; residential clothes dryers; room air conditioners; packaged terminal air...

  4. Global Potential of Energy Efficiency Standards and Labeling Programs

    E-Print Network [OSTI]

    McNeil, Michael A

    2008-01-01

    Chile* China Hong Kong* India* Energy Intensity for Coolingfluorescent lamps in India and Brazil." Energy Policy 19(5):fluorescent lamps (CFLs) in India." Energy Policy 31 : 259-

  5. Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of Botulinum neurotoxin using high-affinity antibodies

    SciTech Connect (OSTI)

    Warner, Marvin G.; Grate, Jay W.; Tyler, Abby J.; Ozanich, Richard M.; Miller, Keith D.; Lou, Jianlong; Marks, James D.; Bruckner-Lea, Cindy J.

    2009-09-01

    A fluorescence sandwich immunoassay using high affinity antibodies and quantum dot (QD) reporters has been developed for detection of botulinum toxin serotype A (BoNT/A). For the development of the assay, a nontoxic recombinant fragment of the holotoxin (BoNT/A-HC-fragment) has been used as a structurally valid simulant for the full toxin molecule. The antibodies used, AR4 and RAZ1, bind to nonoverlapping epitopes present on both the full toxin and on the recombinant fragment. In one format, the immunoassay is carried out in a 96-well plate with detection in a standard plate reader. Detection down to 31 pM of the BoNT/Hc-fragment was demonstrated with a total incubation time of 3 hours, using AR4 as the capture antibody and QD-coupled RAZ1 as the reporter. In a second format, the AR4 capture antibody was coupled to Sepharose beads, and the immunochemical reactions were carried out in microcentrifuge tubes with an incubation time of 1 hour. These beads were subsequently captured and concentrated in a rotating rod “renewable surface” flow cell as part of a sequential injection fluidic system. This flow cell was equipped with a fiber optic system for fluorescence measurements. In PBS buffer solution matrix, the BoNT/A-HC-fragment was detected to concentrations as low as 5 pM using the fluidic measurement approach.

  6. 1998 federal technical standards workshop: Proceedings

    SciTech Connect (OSTI)

    1998-10-01

    The theme for the 1998 workshop was Standards Management -- A World of Change and Opportunities. The workshop`s goal was to further the implementation of the National Technology Transfer and Advancement Act of 1995 (Public Law 104-113) through the sharing of standards management success stories, lessons learned, and emerging initiatives within the Executive Branch of the Federal Government. The target audience for this workshop included agency/department and contractor personnel and representatives of standards developing organizations that either used technical standards in their work for the Federal Government of participated in standards writing/management activities in support of the missions and programs of Federal agencies/departments. As with previous standards workshops sponsored by the DOE, views on the technical subject areas under the workshop theme were solicited from and provided by agency Standards Executives and standards program managers, voluntary standards organizations, and the private sector. This report includes vugraphs of the presentations.

  7. Standards & Testing NIST has a long history of advancing standards and

    E-Print Network [OSTI]

    industry health IT standards and the development of standards needed for the future, such as those provides technical expertise and leverages industry-led, consensus-based standards developmentStandards & Testing NIST has a long history of advancing standards and the use of technology

  8. A Review of the Reflector Compact Fluorescent Lights Technology Procurement Program: Conclusions and Results

    SciTech Connect (OSTI)

    Sandahl, Linda J.; Gilbride, Theresa L.; Ledbetter, Marc R.; McCullough, Jeffrey J.

    2008-05-19

    This report describes a project sponsored by the U.S. Department of Energy (DOE) and implemented by the Pacific Northwest National Laboratory (PNNL), from 2000 to 2007 to improve the performance of reflector type (R-lamp) compact fluorescent lamps (CFLs) and increase their availability throughout the United States by means of a technology development and procurement strategy. In 2000, at the request of the U.S. Department of Energy’s Emerging Technologies Program and its predecessors, the Pacific Northwest National Laboratory undertook a technology procurement seeking R-CFLs that were specifically designed for use in ICAT recessed can fixtures and that met other minimum performance criteria including minimum light output and size restrictions (to ensure they fit in standard residential recessed cans). The technology procurement included two phases. In Phase I, requests for proposals (RFPs) were issued in October 2002 and five manufacturers responded with 12 lamp models. Eight of these models met the minimum requirements and passed the 6-hour short-term test in a simulated ICAT environment. These eight models were subjected to long-term tests of 6,000 or more hours in a simulated ICAT environment. Three of these models passed the short- and long-term tests and were promoted through the program website (www.pnl.gov/rlamps), press releases, and fliers. To increase the number of qualifying models, a second RFP was issued in June 2005. In April 2007, DOE announced that 16 reflector CFL (R-CFL) models by four manufacturers had met all the minimum requirements of Phase 2 of the R-CFL Technology Innovation Competition. PNNL developed both the criteria and the test apparatus design for Elevated Temperature Life Testing (ETLT), which has been included by DOE in its draft ENERGY STAR specifications for the reflector category of CFLs. PNNL promoted the winning lamps through a program website, press releases, and fliers as well as through program partners. PNNL also helped engage distributors including Costco, the Home Depot, Bonneville Power Administration, and utility organizations.

  9. Cost Estimating, Analysis, and Standardization

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1984-11-02

    To establish policy and responsibilities for: (a) developing and reviewing project cost estimates; (b) preparing independent cost estimates and analysis; (c) standardizing cost estimating procedures; and (d) improving overall cost estimating and analytical techniques, cost data bases, cost and economic escalation models, and cost estimating systems. Cancels DOE O 5700.2B, dated 8-5-1983; DOE O 5700.8, dated 5-27-1981; and HQ 1130.1A, dated 12-30-1981. Canceled by DOE O 5700.2D, dated 6-12-1992

  10. Beyond the Standard Model Theory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 OutreachProductswsicloudwsiclouddenDVA N C E D B L O OLaura| National2.11DESERTWaterSharingBeyond the Standard

  11. Standard Terms and Conditions | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation ofAlbuquerque|SensitiveApril 2, 2014ApplicationLanceStaff DeputyStandard Terms

  12. NREL: Photovoltaics Research - Standards Development

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines lightGeospatial Toolkit TheCompetitiveMatt PageEnergyStandards Development

  13. Clear Standards | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar EnergyLawler,Coal Technologies Place:Standards Jump to: navigation, search

  14. Optimized scalable stack of fluorescent solar concentrator systems with bifacial silicon solar cells

    SciTech Connect (OSTI)

    Martínez Díez, Ana Luisa, E-mail: a.martinez@itma.es [Fundación ITMA, Parque Empresarial Principado de Asturias, C/Calafates, Parcela L-3.4, 33417 Avilés (Spain); Fraunhofer Institute for Solar Energy Systems ISE, Heidenhofstr. 2, 79110 Freiburg (Germany); Gutmann, Johannes; Posdziech, Janina; Rist, Tim; Goldschmidt, Jan Christoph [Fraunhofer Institute for Solar Energy Systems ISE, Heidenhofstr. 2, 79110 Freiburg (Germany); Plaza, David Gómez [Fundación ITMA, Parque Empresarial Principado de Asturias, C/Calafates, Parcela L-3.4, 33417 Avilés (Spain)

    2014-10-21

    In this paper, we present a concentrator system based on a stack of fluorescent concentrators (FCs) and a bifacial solar cell. Coupling bifacial solar cells to a stack of FCs increases the performance of the system and preserves its efficiency when scaled. We used an approach to optimize a fluorescent solar concentrator system design based on a stack of multiple fluorescent concentrators (FC). Seven individual fluorescent collectors (20 mm×20 mm×2 mm) were realized by in-situ polymerization and optically characterized in regard to their ability to guide light to the edges. Then, an optimization procedure based on the experimental data of the individual FCs was carried out to determine the stack configuration that maximizes the total number of photons leaving edges. Finally, two fluorescent concentrator systems were realized by attaching bifacial silicon solar cells to the optimized FC stacks: a conventional system, where FC were attached to one side of the solar cell as a reference, and the proposed bifacial configuration. It was found that for the same overall FC area, the bifacial configuration increases the short-circuit current by a factor of 2.2, which is also in agreement with theoretical considerations.

  15. A fluorescence-based method for rapid and direct determination of polybrominated diphenyl ethers in water

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shan, Huimei; Liu, Chongxuan; Wang, Zheming; Ma, Teng; Shang, Jianying; Pan, Duoqiang

    2015-01-01

    A new method was developed for rapid and direct measurement of polybrominated diphenyl ethers (PBDEs) in aqueous samples using fluorescence spectroscopy. The fluorescence spectra of tri- to deca-BDE (BDE 28, 47, 99, 153, 190, and 209) commonly found in environment were measured at variable emission and excitation wavelengths. The results revealed that the PBDEs have distinct fluorescence spectral profiles and peak positions that can be exploited to identify these species and determine their concentrations in aqueous solutions. The detection limits as determined in deionized water spiked with PBDEs are 1.71-5.82 ng/L for BDE 28, BDE 47, BDE 190, and BDEmore »209 and 45.55–69.95 ng/L for BDE 99 and BDE 153. The effects of environmental variables including pH, humic substance, and groundwater chemical composition on PBDEs measurements were also investigated. These environmental variables affected fluorescence intensity, but their effect can be corrected through linear additivity and separation of spectral signal contribution. Compared with conventional GC-based analytical methods, the fluorescence spectroscopy method is more efficient as it only uses a small amount of samples (2-4 mL), avoids lengthy complicated concentration and extraction steps, and has a low detection limit of a few ng/L.« less

  16. A fluorescence-based method for rapid and direct determination of polybrominated diphenyl ethers in water

    SciTech Connect (OSTI)

    Shan, Huimei; Liu, Chongxuan; Wang, Zheming; Ma, Teng; Shang, Jianying; Pan, Duoqiang

    2015-01-01

    A new method was developed for rapid and direct measurement of polybrominated diphenyl ethers (PBDEs) in aqueous samples using fluorescence spectroscopy. The fluorescence spectra of tri- to deca-BDE (BDE 28, 47, 99, 153, 190, and 209) commonly found in environment were measured at variable emission and excitation wavelengths. The results revealed that the PBDEs have distinct fluorescence spectral profiles and peak positions that can be exploited to identify these species and determine their concentrations in aqueous solutions. The detection limits as determined in deionized water spiked with PBDEs are 1.71-5.82 ng/L for BDE 28, BDE 47, BDE 190, and BDE 209 and 45.55–69.95 ng/L for BDE 99 and BDE 153. The effects of environmental variables including pH, humic substance, and groundwater chemical composition on PBDEs measurements were also investigated. These environmental variables affected fluorescence intensity, but their effect can be corrected through linear additivity and separation of spectral signal contribution. Compared with conventional GC-based analytical methods, the fluorescence spectroscopy method is more efficient as it only uses a small amount of samples (2-4 mL), avoids lengthy complicated concentration and extraction steps, and has a low detection limit of a few ng/L.

  17. A Fluorescence-Based Method for Rapid and Direct Determination of Polybrominated Diphenyl Ethers in Water

    SciTech Connect (OSTI)

    Shan, Huimei [China Univ. of Geosciences, Wuhan (China). Lab of Basin and Wetland Eco-Restoration; Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Liu, Chongxuan [China Univ. of Geosciences, Wuhan (China). Lab of Basin and Wetland Eco-Restoration; Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Wang, Zheming [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Ma, Teng [China Univ. of Geosciences, Wuhan (China). Lab of Basin and Wetland Eco-Restoration and State Key Lab. of Biogeology and Environmental Geology; Shang, Jianying [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Pan, Duoqiang [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-01-01

    A new method was developed for rapid and direct measurement of polybrominated diphenyl ethers (PBDEs) in aqueous samples using fluorescence spectroscopy. The fluorescence spectra of tri- to deca-BDE (BDE 28, 47, 99, 153, 190, and 209) commonly found in environment were measured at variable emission and excitation wavelengths. The results revealed that the PBDEs have distinct fluorescence spectral profiles and peak positions that can be exploited to identify these species and determine their concentrations in aqueous solutions. The detection limits as determined in deionized water spiked with PBDEs are 1.71-5.82 ng/L for BDE 28, BDE 47, BDE 190, and BDE 209 and 45.55–69.95 ng/L for BDE 99 and BDE 153. The effects of environmental variables including pH, humic substance, and groundwater chemical composition on PBDEs measurements were also investigated. These environmental variables affected fluorescence intensity, but their effect can be corrected through linear additivity and separation of spectral signal contribution. Compared with conventional GC-based analytical methods, the fluorescence spectroscopy method is more efficient as it only uses a small amount of samples (2-4 mL), avoids lengthy complicated concentration and extraction steps, and has a low detection limit of a few ng/L.

  18. Evaluation of measurement reproducibility using the standard-sites data, 1994 Fernald field characterization demonstration project

    SciTech Connect (OSTI)

    Rautman, C.A.

    1996-02-01

    The US Department of Energy conducted the 1994 Fernald (Ohio) field characterization demonstration project to evaluate the performance of a group of both industry-standard and proposed alternative technologies in describing the nature and extent of uranium contamination in surficial soils. Detector stability and measurement reproducibility under actual operating conditions encountered in the field is critical to establishing the credibility of the proposed alternative characterization methods. Comparability of measured uranium activities to those reported by conventional, US Environmental Protection Agency (EPA)-certified laboratory methods is also required. The eleven (11) technologies demonstrated included (1) EPA-standard soil sampling and laboratory mass-spectroscopy analyses, and currently-accepted field-screening techniques using (2) sodium-iodide scintillometers, (3) FIDLER low-energy scintillometers, and (4) a field-portable x-ray fluorescence spectrometer. Proposed advanced characterization techniques included (5) alpha-track detectors, (6) a high-energy beta scintillometer, (7) electret ionization chambers, (8) and (9) a high-resolution gamma-ray spectrometer in two different configurations, (10) a field-adapted laser ablation-inductively coupled plasma-atomic emission spectroscopy (ICP-AES) technique, and (11) a long-range alpha detector. Measurement reproducibility and the accuracy of each method were tested by acquiring numerous replicate measurements of total uranium activity at each of two ``standard sites`` located within the main field demonstration area. Meteorological variables including temperature, relative humidity. and 24-hour rainfall quantities were also recorded in conjunction with the standard-sites measurements.

  19. Social Responsibility Standards: A Quick Glimpse Although efforts to establish measurements, standards and certifications related to

    E-Print Network [OSTI]

    Aalberts, Daniel P.

    to establish measurements, standards and certifications related to various areas of social: ISO Standard 24000--Social Responsibility: The International Organization't be a certification standard. FOOD LABELING Fairtrade Labelling Organizations

  20. Average M shell fluorescence yields for elements with 70?Z?92

    SciTech Connect (OSTI)

    Kahoul, A.; Deghfel, B.; Aylikci, V.; Aylikci, N. K.; Nekkab, M.

    2015-03-30

    The theoretical, experimental and analytical methods for the calculation of average M-shell fluorescence yield (?{sup ¯}{sub M}) of different elements are very important because of the large number of their applications in various areas of physical chemistry and medical research. In this paper, the bulk of the average M-shell fluorescence yield measurements reported in the literature, covering the period 1955 to 2005 are interpolated by using an analytical function to deduce the empirical average M-shell fluorescence yield in the atomic range of 70?Z?92. The results were compared with the theoretical and fitted values reported by other authors. Reasonable agreement was typically obtained between our result and other works.

  1. Intense Internal and External Fluorescence as Solar Cells Approach the Shockley-Queisser Efficiency Limit

    E-Print Network [OSTI]

    Miller, Owen D; Kurtz, Sarah R

    2012-01-01

    Absorbed sunlight in a solar cell produces electrons and holes. But, at the open circuit condition, the carriers have no place to go. They build up in density and, ideally, they emit external fluorescence that exactly balances the incoming sunlight. Any additional non-radiative recombination impairs the carrier density buildup, limiting the open-circuit voltage. At open-circuit, efficient external fluorescence is an indicator of low internal optical losses. Thus efficient external fluorescence is, counter-intuitively, a necessity for approaching the Shockley-Queisser efficiency limit. A great Solar Cell also needs to be a great Light Emitting Diode. Owing to the narrow escape cone for light, efficient external emission requires repeated attempts, and demands an internal luminescence efficiency >>90%.

  2. Fluorescence measurements for evaluating the application of multivariate analysis techniques to optically thick environments.

    SciTech Connect (OSTI)

    Reichardt, Thomas A.; Timlin, Jerilyn Ann; Jones, Howland D. T.; Sickafoose, Shane M.; Schmitt, Randal L.

    2010-09-01

    Laser-induced fluorescence measurements of cuvette-contained laser dye mixtures are made for evaluation of multivariate analysis techniques to optically thick environments. Nine mixtures of Coumarin 500 and Rhodamine 610 are analyzed, as well as the pure dyes. For each sample, the cuvette is positioned on a two-axis translation stage to allow the interrogation at different spatial locations, allowing the examination of both primary (absorption of the laser light) and secondary (absorption of the fluorescence) inner filter effects. In addition to these expected inner filter effects, we find evidence that a portion of the absorbed fluorescence is re-emitted. A total of 688 spectra are acquired for the evaluation of multivariate analysis approaches to account for nonlinear effects.

  3. Demonstration of x-ray fluorescence imaging of a high-energy-density plasma

    SciTech Connect (OSTI)

    MacDonald, M. J., E-mail: macdonm@umich.edu; Gamboa, E. J. [Department of Atmospheric, Oceanic, and Space Sciences, University of Michigan, Ann Arbor, Michigan 48109 (United States); SLAC National Accelerator Laboratory, Menlo Park, California 94025 (United States); Keiter, P. A.; Fein, J. R.; Klein, S. R.; Kuranz, C. C.; LeFevre, H. J.; Manuel, M. J.-E.; Wan, W. C.; Drake, R. P. [Department of Atmospheric, Oceanic, and Space Sciences, University of Michigan, Ann Arbor, Michigan 48109 (United States); Montgomery, D. S. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States); Biener, M. M.; Fournier, K. B. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Streit, J. [Schafer Corporation, Livermore, California 94551 (United States)

    2014-11-15

    Experiments at the Trident Laser Facility have successfully demonstrated the use of x-ray fluorescence imaging (XRFI) to diagnose shocked carbonized resorcinol formaldehyde (CRF) foams doped with Ti. One laser beam created a shock wave in the doped foam. A second laser beam produced a flux of vanadium He-? x-rays, which in turn induced Ti K-shell fluorescence within the foam. Spectrally resolved 1D imaging of the x-ray fluorescence provided shock location and compression measurements. Additionally, experiments using a collimator demonstrated that one can probe specific regions within a target. These results show that XRFI is a capable alternative to path-integrated measurements for diagnosing hydrodynamic experiments at high energy density.

  4. Quantitative measurement of binary liquid distributions using multiple-tracer x-ray fluorescence and radiography

    SciTech Connect (OSTI)

    Halls, Benjamin R.; Meyer, Terrence R.; Kastengren, Alan L.

    2015-01-01

    The complex geometry and large index-of-refraction gradients that occur near the point of impingement of binary liquid jets present a challenging environment for optical interrogation. A simultaneous quadruple-tracer x-ray fluorescence and line-of-sight radiography technique is proposed as a means of distinguishing and quantifying individual liquid component distributions prior to, during, and after jet impact. Two different pairs of fluorescence tracers are seeded into each liquid stream to maximize their attenuation ratio for reabsorption correction and differentiation of the two fluids during mixing. This approach for instantaneous correction of x-ray fluorescence reabsorption is compared with a more time-intensive approach of using stereographic reconstruction of x-ray attenuation along multiple lines of sight. The proposed methodology addresses the need for a quantitative measurement technique capable of interrogating optically complex, near-field liquid distributions in many mixing systems of practical interest involving two or more liquid streams.

  5. Quantitative measurement of binary liquid distributions using multiple-tracer x-ray fluorescence and radiography

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Halls, Benjamin R.; Meyer, Terrence R.; Kastengren, Alan L.

    2015-01-23

    The complex geometry and large index-of-refraction gradients that occur near the point of impingement of binary liquid jets present a challenging environment for optical interrogation. A simultaneous quadruple-tracer x-ray fluorescence and line-of-sight radiography technique is proposed as a means of distinguishing and quantifying individual liquid component distributions prior to, during, and after jet impact. Two different pairs of fluorescence tracers are seeded into each liquid stream to maximize their attenuation ratio for reabsorption correction and differentiation of the two fluids during mixing. This approach for instantaneous correction of x-ray fluorescence reabsorption is compared with a more time-intensive approach of usingmore »stereographic reconstruction of x-ray attenuation along multiple lines of sight. The proposed methodology addresses the need for a quantitative measurement technique capable of interrogating optically complex, near-field liquid distributions in many mixing systems of practical interest involving two or more liquid streams.« less

  6. Information Technology: American National Standard for

    E-Print Network [OSTI]

    Information Technology: American National Standard for Information Systems-- Data Format Publication 500-245 Information Technology: American National Standard for Information Systems-- Data Format for the Interchange of Fingerprint, Facial, & Scar Mark & Tattoo (SMT) Information Sponsored by Information Technology

  7. JOHNS HOPKINS UNIVERSITY COST ACCOUNTING STANDARDS BOARD

    E-Print Network [OSTI]

    JOHNS HOPKINS UNIVERSITY COST ACCOUNTING STANDARDS BOARD DISCLOSURE STATEMENT FOR EDUCATIONAL INSTITUTIONS CASB DS-2 March 23, 2010 #12;COST ACCOUNTING STANDARDS BOARD DISCLOSURE STATEMENT REQUIRED BY PUBLIC LAW 100-679 EDUCATIONAL INSTITUTIONS INDEX JOHNS HOPKINS UNIVERISTY GENERAL INSTRUCTIONS

  8. A Pragmatic Standard of Legal Validity 

    E-Print Network [OSTI]

    Tyler, John

    2012-07-16

    American jurisprudence currently applies two incompatible validity standards to determine which laws are enforceable. The natural law tradition evaluates validity by an uncertain standard of divine law, and its methodology relies on contradictory...

  9. Energy Efficiency Standards for State Buildings

    Broader source: Energy.gov [DOE]

    The Montana Department of Labor must also develop and adopt high performance building standards, along with the Montana university system and other state agencies. These standards must take into...

  10. Late standardization and technological catch-up

    E-Print Network [OSTI]

    Ratanawaraha, Apiwat, 1972-

    2006-01-01

    In this study, we examine the process of "late standardization," in which latecomers engage in standards activities in order to move towards and beyond the technological frontier. Based on case studies of latecomers in the ...

  11. Information Technology: American National Standard for Information

    E-Print Network [OSTI]

    Information Technology: American National Standard for Information Systems-- Data Format 500-275 Information Technology: American National Standard for Information Systems-- Data Format Coleman, and Patrice Yuh, Editors Sponsored by Information Access Division Information Technology

  12. New and revised standards for coke production

    SciTech Connect (OSTI)

    G.A. Kotsyuba; M.I. Alpatov; Y.G. Shapoval [Giprokoks, the State Institute for the Design of Coke-Industry Enterprises, Kharkov (Ukraine)

    2009-07-15

    The need for new and revised standards for coke production in Ukraine and Russia is outlined. Such standards should address improvements in plant operation, working conditions, environmental protection, energy conservation, fire and explosion safety, and economic indices.

  13. Setting the Standard for Industrial Energy Efficiency

    E-Print Network [OSTI]

    McKane, Aimee; Williams, Robert; Perry, Wayne; Li, Tienan

    2008-01-01

    National Standards Institute (ANSI), it has received littleversion of the Georgia Tech/ANSI energy management standard.developed by Georgia Tech/ANSI, was based on ISO management

  14. Measurements and Standards for Thermoelectric Materials

    E-Print Network [OSTI]

    Measurements and Standards for Thermoelectric Materials CERAMICS Our goal is to develop standard, electrical conductivity, thermal conductivity) for thin film and bulk thermoelectric materials to enable approach will facilitate comparison of thermoelectric data between leading laboratories, and accelerate

  15. Charge Transfer Fluorescence and 34 nm Exciton Diffusion Length in Polymers with Electron Acceptor End Traps

    SciTech Connect (OSTI)

    Zaikowski, L.; Mauro, G.; Bird, M.; Karten, B.; Asaoka, S.; Wu, Q.; Cook, A. R.; Miller, J.

    2014-12-22

    Photoexcitation of conjugated poly-2,7-(9,9-dihexylfluorene) polyfluorenes with naphthylimide (NI) and anthraquinone (AQ) electron-acceptor end traps produces excitons that form charge transfer states at the end traps. Intramolecular singlet exciton transport to end traps was examined by steady state fluorescence for polyfluorenes of 17 to 127 repeat units in chloroform, dimethylformamide (DMF), tetrahydrofuran (THF), and p-xylene. End traps capture excitons and form charge transfer (CT) states at all polymer lengths and in all solvents. The CT nature of the end-trapped states is confirmed by their fluorescence spectra, solvent and trap group dependence and DFT descriptions. Quantum yields of CT fluorescence are as large as 46%. This strong CT emission is understood in terms of intensity borrowing. Energies of the CT states from onsets of the fluorescence spectra give the depths of the traps which vary with solvent polarity. For NI end traps the trap depths are 0.06 (p-xylene), 0.13 (THF) and 0.19 eV (CHCl3). For AQ, CT fluorescence could be observed only in p-xylene where the trap depth is 0.27 eV. Quantum yields, emission energies, charge transfer energies, solvent reorganization and vibrational energies were calculated. Fluorescence measurements on chains >100 repeat units indicate that end traps capture ~50% of the excitons, and that the exciton diffusion length LD =34 nm, which is much larger than diffusion lengths reported in polymer films or than previously known for diffusion along isolated chains. The efficiency of exciton capture depends on chain length, but not on trap depth, solvent polarity or which trap group is present.

  16. A portable time-domain LED fluorimeter for nanosecond fluorescence lifetime measurements

    SciTech Connect (OSTI)

    Wang, Hongtao; Salthouse, Christopher D., E-mail: salthouse@ecs.umass.edu [Electrical and Computer Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Qi, Ying; Mountziaris, T. J. [Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States) [Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Chemical Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 (United States)

    2014-05-15

    Fluorescence lifetime measurements are becoming increasingly important in chemical and biological research. Time-domain lifetime measurements offer fluorescence multiplexing and improved handling of interferers compared with the frequency-domain technique. In this paper, an all solid-state, filterless, and highly portable light-emitting-diode based time-domain fluorimeter (LED TDF) is reported for the measurement of nanosecond fluorescence lifetimes. LED based excitation provides more wavelengths options compared to laser diode based excitation, but the excitation is less effective due to the uncollimated beam, less optical power, and longer latency in state transition. Pulse triggering and pre-bias techniques were implemented in our LED TDF to improve the peak optical power to over 100 mW. The proposed pulsing circuit achieved an excitation light fall time of less than 2 ns. Electrical resetting technique realized a time-gated photo-detector to remove the interference of the excitation light with fluorescence. These techniques allow the LED fluorimeter to accurately measure the fluorescence lifetime of fluorescein down to concentration of 0.5 ?M. In addition, all filters required in traditional instruments are eliminated for the non-attenuated excitation/emission light power. These achievements make the reported device attractive to biochemical laboratories seeking for highly portable lifetime detection devices for developing sensors based on fluorescence lifetime changes. The device was initially validated by measuring the lifetimes of three commercial fluorophores and comparing them with reported lifetime data. It was subsequently used to characterize a ZnSe quantum dot based DNA sensor.

  17. Charge transfer fluorescence and 34 nm exciton diffusion length in polymers with electron acceptor end traps

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zaikowski, Lori; Mauro, Gina; Bird, Matthew; Karten, Brianne; Asaoka, Sadayuki; Wu, Qin; Cook, Andrew R.; Miller, John R.

    2014-12-22

    Photoexcitation of conjugated poly-2,7-(9,9-dihexylfluorene) polyfluorenes with naphthylimide (NI) and anthraquinone (AQ) electron-acceptor end traps produces excitons that form charge transfer states at the end traps. Intramolecular singlet exciton transport to end traps was examined by steady state fluorescence for polyfluorenes of 17 to 127 repeat units in chloroform, dimethylformamide (DMF), tetrahydrofuran (THF), and p-xylene. End traps capture excitons and form charge transfer (CT) states at all polymer lengths and in all solvents. The CT nature of the end-trapped states is confirmed by their fluorescence spectra, solvent and trap group dependence and DFT descriptions. Quantum yields of CT fluorescence are asmore »large as 46%. This strong CT emission is understood in terms of intensity borrowing. Energies of the CT states from onsets of the fluorescence spectra give the depths of the traps which vary with solvent polarity. For NI end traps the trap depths are 0.06 (p-xylene), 0.13 (THF) and 0.19 eV (CHCl3). For AQ, CT fluorescence could be observed only in p-xylene where the trap depth is 0.27 eV. Quantum yields, emission energies, charge transfer energies, solvent reorganization and vibrational energies were calculated. Fluorescence measurements on chains >100 repeat units indicate that end traps capture ~50% of the excitons, and that the exciton diffusion length LD =34 nm, which is much larger than diffusion lengths reported in polymer films or than previously known for diffusion along isolated chains. As a result, the efficiency of exciton capture depends on chain length, but not on trap depth, solvent polarity or which trap group is present.« less

  18. The Building Standard (Scotland) Amendment Regulations 1964 

    E-Print Network [OSTI]

    Noble, Michael

    1964-01-01

    STATUTORY INSTRUMENTS 1964 No. 802 (S. 50) BUILDING AND BUILDINGS The Building Standards (Scotland) Amendment Regulations 1964

  19. EPA's Renewable Fuels Standard Web page

    SciTech Connect (OSTI)

    2011-12-30

    The Renewable Fuel Standard (RFS) program regulations were developed in collaboration with refiners, renewable fuel producers, and many other stakeholders.

  20. ANS Standards to Support DOE NPH Design

    Office of Energy Efficiency and Renewable Energy (EERE)

    ANS Standards to Support DOE NPH Design DOE NPH Workshop, Germantown, MD Carl Mazzola, CFS October 22, 2014

  1. Webtrends Archives by Fiscal Year — Communication Standards

    Office of Energy Efficiency and Renewable Energy (EERE)

    From the EERE Web Statistics Archive: Corporate sites, Webtrends archives for the Communication Standards site by fiscal year.

  2. 1996 DOE technical standards program workshop: Proceedings

    SciTech Connect (OSTI)

    NONE

    1996-07-01

    The workshop theme is `The Strategic Standardization Initiative - A Technology Exchange and Global Competitiveness Challenge for DOE.` The workshop goal is to inform the DOE technical standards community of strategic standardization activities taking place in the Department, other Government agencies, standards developing organizations, and industry. Individuals working on technical standards will be challenged to improve cooperation and communications with the involved organizations in response to the initiative. Workshop sessions include presentations by representatives from various Government agencies that focus on coordination among and participation of Government personnel in the voluntary standards process; reports by standards organizations, industry, and DOE representatives on current technology exchange programs; and how the road ahead appears for `information superhighway` standardization. Another session highlights successful standardization case studies selected from several sites across the DOE complex. The workshop concludes with a panel discussion on the goals and objectives of the DOE Technical Standards Program as envisioned by senior DOE management. The annual workshop on technical standards has proven to be an effective medium for communicating information related to standards throughout the DOE community. Technical standards are used to transfer technology and standardize work processes to produce consistent, acceptable results. They provide a practical solution to the Department`s challenge to protect the environment and the health and safety of the public and workers during all facility operations. Through standards, the technologies of industries and governments worldwide are available to DOE. The DOE Technical Standards Program, a Department-wide effort that crosscuts all organizations and disciplines, links the Department to those technologies.

  3. Multi-objective fuel policies: Renewable fuel standards versus Fuel greenhouse gas intensity standards

    E-Print Network [OSTI]

    Rajagopal, Deepak

    2010-01-01

    Gas Reductions under Low Carbon Fuel Standards? Americanto Implement the Low Carbon Fuel Standard, Volume I Sta?Paper Series Multi-objective fuel policies: Renewable fuel

  4. Living up to standards Margaret King

    E-Print Network [OSTI]

    to record my thanks to Nigel Bevan, technical editor of the ISO standards discussed for much interestingLiving up to standards Margaret King TIM/ISSCO ETI University of Geneva Margaret reusable" by arguing that a set of ISO standards developed for the evaluation of software in general

  5. NISTIR 7977 NIST Cryptographic Standards and

    E-Print Network [OSTI]

    by providing technical leadership for the Nation's measurement and standards infrastructure. ITL develops tests of management, administrative, technical, and physical standards and guidelines for the cost-effective securityNISTIR 7977 NIST Cryptographic Standards and Guidelines Development Process (Second Draft

  6. Improving the Security of Cryptographic Protocol Standards

    E-Print Network [OSTI]

    Basin, David

    for their implementation. This generality is enabled by standards and technical documents such as Request for CommentsImproving the Security of Cryptographic Protocol Standards David Basin Cas Cremers Kunihiko Miyazaki Sasa Radomirovic Dai Watanabe May 1, 2014 Keywords: Standards, Network Protocols, Security

  7. NIST Cryptographic Standards and Guidelines Development Process

    E-Print Network [OSTI]

    Rivest, Ronald L.

    as technical experts in the review of National Institute of Standards and Technology (NIST) cryptographicNIST Cryptographic Standards and Guidelines Development Process Report and Recommendations of the Visiting Committee on Advanced Technology of the National Institute of Standards and Technology July 2014

  8. NIST Standards in Trade Workshops Increase

    E-Print Network [OSTI]

    NIST Standards in Trade Workshops Increase Trade Opportunities and Exports for U.S. Industry Since 1995, U.S. industry has looked to the NIST Standards in Trade (SIT) Workshop Program to provide opportunities for cooperation on topics related to standards, conformity assessment and trade that are important

  9. Method for detecting binding events using micro-X-ray fluorescence spectrometry

    DOE Patents [OSTI]

    Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Mann, Grace (Hong Kong, HK)

    2010-12-28

    Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

  10. Nanodiamonds with silicon vacancy defects for non-toxic photostable fluorescent labeling of neural precursor cells

    E-Print Network [OSTI]

    Merson, Tobias D; Aharonovich, Igor; Turbic, Alisa; Kilpatrick, Trevor J; Turnley, Ann M

    2013-01-01

    Nanodiamonds (NDs) containing silicon vacancy (SiV) defects were evaluated as a potential biomarker for the labeling and fluorescent imaging of neural precursor cells (NPCs). SiV-containing NDs were synthesized using chemical vapor deposition and silicon ion implantation. Spectrally, SiV-containing NDs exhibited extremely stable fluorescence and narrow bandwidth emission with an excellent signal to noise ratio exceeding that of NDs containing nitrogen-vacancy (NV) centers. NPCs labeled with NDs exhibited normal cell viability and proliferative properties consistent with biocompatibility. We conclude that SiVcontaining NDs are a promising biomedical research tool for cellular labeling and optical imaging in stem cell research.

  11. Fast repetition rate (FRR) fluorometer and method for measuring fluorescence and photosynthetic parameters

    DOE Patents [OSTI]

    Kolber, Z.; Falkowski, P.

    1995-06-20

    A fast repetition rate fluorometer device and method for measuring in vivo fluorescence of phytoplankton or higher plants chlorophyll and photosynthetic parameters of phytoplankton or higher plants is revealed. The phytoplankton or higher plants are illuminated with a series of fast repetition rate excitation flashes effective to bring about and measure resultant changes in fluorescence yield of their Photosystem II. The series of fast repetition rate excitation flashes has a predetermined energy per flash and a rate greater than 10,000 Hz. Also, disclosed is a flasher circuit for producing the series of fast repetition rate flashes. 14 figs.

  12. High efficiency and brightness fluorescent organic light emitting diode by triplet-triplet fusion

    DOE Patents [OSTI]

    Forrest, Stephen; Zhang, Yifan

    2015-02-10

    A first device is provided. The first device further comprises an organic light emitting device. The organic light emitting device further comprises an anode, a cathode, and an emissive layer disposed between the anode and the cathode. The emissive layer may include an organic host compound and at least one organic emitting compound capable of fluorescent emission at room temperature. Various configurations are described for providing a range of current densities in which T-T fusion dominates over S-T annihilation, leading to very high efficiency fluorescent OLEDs.

  13. Measurement of Pressure Dependent Fluorescence Yield of Air: Calibration Factor for UHECR Detectors

    SciTech Connect (OSTI)

    Belz, J.W.; Burt, G.W.; Cao, Z.; Chang, F.Y.; Chen, C.C.; Chen, C.W.; Chen, P.; Field, C.; Findlay, J.; Huntemeyer, Petra; Huang, M.A.; Hwang, W.-Y.P.; Iverson, R.; Jones, B.F.; Jui, C.C.H.; Kirn, M.; Lin, G.-L.; Loh, E.C.; Maestas, M.M.; Manago, N.; Martens, K.; /Montana U. /Utah U. /Taiwan, Natl. Taiwan U. /SLAC /Rutgers U., Piscataway

    2005-07-06

    In a test experiment at the Final Focus Test Beam of the Stanford Linear Accelerator Center, the fluorescence yield of 28.5 GeV electrons in air and nitrogen was measured. The measured photon yields between 300 and 400 nm at 1 atm and 29 C are Y(760 Torr){sup air} = 4.42 {+-} 0.73 and Y(760 Torr){sup N{sub 2}} = 29.2 {+-} 4.8 photons per electron per meter. Assuming that the fluorescence yield is proportional to the energy deposition of a charged particle traveling through air, good agreement with measurements at lower particle energies is observed.

  14. Three-dimensional x-ray fluorescence mapping of a gold nanoparticle-loaded phantom

    SciTech Connect (OSTI)

    Ren, Liqiang; Wu, Di; Li, Yuhua; Liu, Hong, E-mail: liu@ou.edu [Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, Oklahoma 73019 (United States)] [Center for Bioengineering and School of Electrical and Computer Engineering, University of Oklahoma, Norman, Oklahoma 73019 (United States); Wang, Ge [Biomedical Imaging Cluster and Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180 (United States)] [Biomedical Imaging Cluster and Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180 (United States); Wu, Xizeng [Department of Radiology, University of Alabama, Birmingham, Alabama 35233 (United States)] [Department of Radiology, University of Alabama, Birmingham, Alabama 35233 (United States)

    2014-03-15

    Purpose : X-ray fluorescence (XRF) is a promising technique with sufficient specificity and sensitivity for identifying and quantifying features in small samples containing high atomic number (Z) materials such as iodine, gadolinium, and gold. In this study, the feasibility of applying XRF to early breast cancer diagnosis and treatment is studied using a novel approach for three-dimensional (3D) x-ray fluorescence mapping (XFM) of gold nanoparticle (GNP)-loaded objects in a physical phantom at the technical level. Methods : All the theoretical analysis and experiments are conducted under the condition of using x-ray pencil beam and a compactly integrated x-ray spectrometer. The penetrability of the fluorescence x-rays from GNPs is first investigated by adopting a combination of BR12 with 70 mm/50 mm in thickness on the excitation/emission path to mimic the possible position of tumor goldin vivo. Then, a physical phantom made of BR12 is designed to translate in 3D space with three precise linear stages and subsequently the step by step XFM scanning is performed. The experimental technique named as background subtraction is applied to isolate the gold fluorescence from each spectrum obtained by the spectrometer. Afterwards, the attenuations of both the incident primary x-ray beam with energies beyond the gold K-edge energy (80.725 keV) and the isolated gold K{sub ?} fluorescence x-rays (65.99 –69.80 keV) acquired after background subtraction are well calibrated, and finally the unattenuated K{sub ?} fluorescence counts are used to realize mapping reconstruction and to describe the linear relationship between gold fluorescence counts and corresponding concentration of gold solutions. Results : The penetration results show that the goldK{sub ?} fluorescence x-rays have sufficient penetrability for this phantom study, and the reconstructed mapping results indicate that both the spatial distribution and relative concentration of GNPs within the designed BR12 phantom can be well identified and quantified. Conclusions : Although the XFM method in this investigation is still studied at the technical level and is not yet practical for routinein vivo mapping tasks with GNPs, the current penetrability measurements and phantom study strongly suggest the feasibility to establish and develop a 3D XFM system.

  15. A new standard pulsar magnetosphere

    SciTech Connect (OSTI)

    Contopoulos, Ioannis [Research Center for Astronomy and Applied Mathematics, Academy of Athens, Athens 11527 (Greece); Kalapotharakos, Constantinos [University of Maryland, College Park (UMDCP/CRESST), College Park, MD 20742 (United States); Kazanas, Demosthenes, E-mail: icontop@academyofathens.gr [Astrophysics Science Division, NASA/Goddard Space Flight Center, Greenbelt, MD 20771 (United States)

    2014-01-20

    In view of recent efforts to probe the physical conditions in the pulsar current sheet, we revisit the standard solution that describes the main elements of the ideal force-free pulsar magnetosphere. The simple physical requirement that the electric current contained in the current layer consists of the local electric charge moving outward at close to the speed of light yields a new solution for the pulsar magnetosphere everywhere that is ideal force-free except in the current layer. The main elements of the new solution are as follows: (1) the pulsar spindown rate of the aligned rotator is 23% larger than that of the orthogonal vacuum rotator; (2) only 60% of the magnetic flux that crosses the light cylinder opens up to infinity; (3) the electric current closes along the other 40%, which gradually converges to the equator; (4) this transfers 40% of the total pulsar spindown energy flux in the equatorial current sheet, which is then dissipated in the acceleration of particles and in high-energy electromagnetic radiation; and (5) there is no separatrix current layer. Our solution is a minimum free-parameter solution in that the equatorial current layer is electrostatically supported against collapse and thus does not require a thermal particle population. In this respect, it is one more step toward the development of a new standard solution. We discuss the implications for intermittent pulsars and long-duration gamma-ray bursts. We conclude that the physical conditions in the equatorial current layer determine the global structure of the pulsar magnetosphere.

  16. National Agenda for Hydrogen Codes and Standards

    SciTech Connect (OSTI)

    Blake, C.

    2010-05-01

    This paper provides an overview of hydrogen codes and standards with an emphasis on the national effort supported and managed by the U.S. Department of Energy (DOE). With the help and cooperation of standards and model code development organizations, industry, and other interested parties, DOE has established a coordinated national agenda for hydrogen and fuel cell codes and standards. With the adoption of the Research, Development, and Demonstration Roadmap and with its implementation through the Codes and Standards Technical Team, DOE helps strengthen the scientific basis for requirements incorporated in codes and standards that, in turn, will facilitate international market receptivity for hydrogen and fuel cell technologies.

  17. Flooding and Phytophthora cinnamomi: Effects on photosynthesis and chlorophyll fluorescence in shoots of non-grafted Persea americana

    E-Print Network [OSTI]

    Flooding and Phytophthora cinnamomi: Effects on photosynthesis and chlorophyll fluorescence: Phytophthora Avocado Fluorescence Gas exchange Flooding Losses in the production of avocado (Persea americana in tree-dieback and eventual tree death. Avocado is also a flood-sensitive species and flooding

  18. Proton Pathways in Green Fluorescence Protein Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

    E-Print Network [OSTI]

    Agmon, Noam

    Proton Pathways in Green Fluorescence Protein Noam Agmon Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel ABSTRACT Proton pathways in green fluorescent protein pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton

  19. Three-Dimensional Fluorescence Spectra of Thermally Stressed Commercial Jet A-1 Aviation Fuel in the Autoxidative Regime

    E-Print Network [OSTI]

    Gülder, Ömer L.

    Three-Dimensional Fluorescence Spectra of Thermally Stressed Commercial Jet A-1 Aviation Fuel: In this study, the thermal oxidative stability of a kerosene-type Jet A-1 commercial aviation fuel has been investigated using a three-dimensional (3D) excitation/emission matrix fluorescence (EEMF) method. The fuel

  20. Inferred influence of nutrient availability on the relationship between Sun-induced chlorophyll fluorescence and incident irradiance in the

    E-Print Network [OSTI]

    Kelley, Dan

    fluorescence and incident irradiance in the Bering Sea Christina Schallenberg,1 Marlon R. Lewis,1 Dan E. Kelley fluorescence and incident solar irradiance as a potential diagnostic of the nutritional status of phytoplankton normalized to the absorption of light by pigments were analyzed as a function of irradiance to describe