National Library of Energy BETA

Sample records for human skin tissue

  1. The Effects of Low Dose Irradiation on Inflammatory Response Proteins in a 3D Reconstituted Human Skin Tissue Model

    SciTech Connect (OSTI)

    Varnum, Susan M.; Springer, David L.; Chaffee, Mary E.; Lien, Katie A.; Webb-Robertson, Bobbie-Jo M.; Waters, Katrina M.; Sacksteder, Colette A.

    2012-12-01

    Skin responses to moderate and high doses of ionizing radiation include the induction of DNA repair, apoptosis, and stress response pathways. Additionally, numerous studies indicate that radiation exposure leads to inflammatory responses in skin cells and tissue. However, the inflammatory response of skin tissue to low dose radiation (<10 cGy) is poorly understood. In order to address this, we have utilized a reconstituted human skin tissue model (MatTek EpiDerm FT) and assessed changes in 23 cytokines twenty-four and forty eight hours following treatment of skin with either 3 or 10 cGy low-dose of radiation. Three cytokines, IFN-?, IL-2, MIP-1?, were significantly altered in response to low dose radiation. In contrast, seven cytokines were significantly altered in response to a high radiation dose of 200 cGy (IL-2, IL-10, IL-13, IFN-?, MIP-1?, TNF ?, and VEGF) or the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (G-CSF, GM-CSF, IL-1?, IL-8, MIP-1?, MIP-1?, RANTES). Additionally, radiation induced inflammation appears to have a distinct cytokine response relative to the non-radiation induced stressor, TPA. Overall, these results indicate that there are subtle changes in the inflammatory protein levels following exposure to low dose radiation and this response is a sub-set of what is seen following a high dose in a human skin tissue model.

  2. In vitro dermal absorption of pyrethroid pesticides in human and rat skin

    SciTech Connect (OSTI)

    Hughes, Michael F.; Edwards, Brenda C.

    2010-07-15

    Dermal exposure to pyrethroid pesticides can occur during manufacture and application. This study examined the in vitro dermal absorption of pyrethroids using rat and human skin. Dermatomed skin from adult male Long Evans rats or human cadavers was mounted in flow-through diffusion cells, and radiolabeled bifenthrin, deltamethrin or cis-permethrin was applied in acetone to the skin. Fractions of receptor fluid were collected every 4 h. At 24 h, the skins were washed with soap and water to remove unabsorbed chemical. The skin was then solubilized. Two additional experiments were performed after washing the skin; the first was tape-stripping the skin and the second was the collection of receptor fluid for an additional 24 h. Receptor fluid, skin washes, tape strips and skin were analyzed for radioactivity. For rat skin, the wash removed 53-71% of the dose and 26-43% remained in the skin. The cumulative percentage of the dose at 24 h in the receptor fluid ranged from 1 to 5%. For human skin, the wash removed 71-83% of the dose and 14-25% remained in the skin. The cumulative percentage of the dose at 24 h in the receptor fluid was 1-2%. Tape-stripping removed 50-56% and 79-95% of the dose in rat and human skin, respectively, after the wash. From 24-48 h, 1-3% and about 1% of the dose diffused into the receptor fluid of rat and human skin, respectively. The pyrethroids bifenthrin, deltamethrin and cis-permethrin penetrated rat and human skin following dermal application in vitro. However, a skin wash removed 50% or more of the dose from rat and human skin. Rat skin was more permeable to the pyrethroids than human skin. Of the dose in skin, 50% or more was removed by tape-stripping, suggesting that permeation of pyrethroids into viable tissue could be impeded. The percentage of the dose absorbed into the receptor fluid was considerably less than the dose in rat and human skin. Therefore, consideration of the skin type used and fractions analyzed are important when using in vitro dermal absorption data for risk assessment.

  3. Screen-Space Perceptual Rendering of Human Skin JORGE JIMENEZ

    E-Print Network [OSTI]

    Gutierrez, Diego

    SUNDSTEDT Trinity College Dublin and DIEGO GUTIERREZ Universidad de Zaragoza We propose a novel skin shader., Sundstedt, V., and Gutierrez, D. 2009. Screen-Space perceptual rendering of human skin. ACM Trans. Appl.Sundstedt@cs.tcd.ie; D. Gutierrez, Departamento de Inform´atica e Ingenier´ia de Sistemas, Universidad de Zaragoza

  4. Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering

    E-Print Network [OSTI]

    Sun, Yu

    , Ontario M5S 3G8, Canada *S Supporting Information ABSTRACT: Electrospinning is a technique for creating into the scaffold. KEYWORDS: electrospining, suspending scaffold, scaffold shrinkage, skin tissue engineering and self-assembly,18 electrospinning is a versatile technique for producing tissue engineering scaffolds

  5. The effect of low dose ionizing radiation on homeostasis and functional integrity in an organotypic human skin model

    SciTech Connect (OSTI)

    von Neubeck, Claere; Geniza, Matthew; Kauer, Paula M.; Robinson, Joseph E.; Chrisler, William B.; Sowa, Marianne B.

    2015-05-01

    Outside the protection of earths atmosphere, astronauts are exposed to low doses of high linear energy transfer (LET) radiation. Future NASA plans for deep space missions or a permanent settlement on the moon are limited by the health risks associated with space radiation exposures. There is a paucity of direct epidemiological data for low dose exposures to space radiation-relevant high LET ions. Health risk models are used to estimate the risk for such exposures, though these models are based on high dose experiments. There is increasing evidence, however, that low and high dose exposures result in different signaling events at the molecular level, and may involve different response mechanisms. Further, despite their low abundance, high LET particles have been identified as the major contributor to health risk during manned space flight. The human skin is exposed in every external radiation scenario, making it an ideal epithelial tissue model in which to study radiation induced effects. Here, we exposed an in vitro three dimensional (3-D) human organotypic skin tissue model to low doses of high LET oxygen (O), silicon (Si) and iron (Fe) ions. We measured proliferation and differentiation profiles in the skin tissue and examined the integrity of the skins barrier function. We discuss the role of secondary particles in changing the proportion of cells receiving a radiation dose, emphasizing the possible impact on radiation-induced health issues in astronauts.

  6. Quantitative Proteomic Profiling of Low Dose Ionizing Radiation Effects in a Human Skin Model

    SciTech Connect (OSTI)

    Hengel, Shawna; Aldrich, Joshua T.; Waters, Katrina M.; Pasa-Tolic, Ljiljana; Stenoien, David L.

    2014-07-29

    To assess molecular responses to low doses of radiation that may be encountered during medical diagnostic procedures, nuclear accidents, or terrorist acts, a quantitative global proteomic approach was used to identify protein alterations in a reconstituted human skin tissue treated with 10 cGy of ionizing radiation. Subcellular fractionation was employed to remove highly abundant structural proteins and provide insight on radiation induced alterations in protein abundance and localization. In addition, peptides were post-fractionated using high resolution 2-dimensional liquid chromatography to increase the dynamic range of detection of protein abundance and translocation changes. Quantitative data was obtained by labeling peptides with 8-plex isobaric iTRAQ tags. A total of 207 proteins were detected with statistically significant alterations in abundance and/or subcellular localization compared to sham irradiated tissues. Bioinformatics analysis of the data indicated that the top canonical pathways affected by low dose radiation are related to cellular metabolism. Among the proteins showing alterations in abundance, localization and proteolytic processing was the skin barrier protein filaggrin which is consistent with our previous observation that ionizing radiation alters profilaggrin processing with potential effects on skin barrier functions. In addition, a large number of proteases and protease regulators were affected by low dose radiation exposure indicating that altered proteolytic activity may be a hallmark of low dose radiation exposure. While several studies have demonstrated altered transcriptional regulation occurs following low dose radiation exposures, the data presented here indicates post-transcriptional regulation of protein abundance, localization, and proteolytic processing play an important role in regulating radiation responses in complex human tissues.

  7. Skin electronics is one of the most promising applications of stretchable electronics. The versatility of skin electronics can only be guaranteed when it has conformal contact with human

    E-Print Network [OSTI]

    Skin electronics is one of the most promising applications of stretchable electronics. The versatility of skin electronics can only be guaranteed when it has conformal contact with human skin. While analysis for the conformability of skin electronics, including modeling, meshing method and step setup etc

  8. Deep sequencing of small RNAs from human skin reveals major alterations

    E-Print Network [OSTI]

    Zhang, Weixiong

    Deep sequencing of small RNAs from human skin reveals major alterations in the psoriasis mi Psoriasis is a chronic and complex inflammatory skin disease with lesions displaying dramatically altered m for functional characterization of miRNAs in healthy and diseased skin. INTRODUCTION Psoriasis (PS) is a chronic

  9. Genome Wide Evaluation of Normal Human Tissue in Response to...

    Office of Scientific and Technical Information (OSTI)

    Wide Evaluation of Normal Human Tissue in Response to Controlled, In vivo Low-Dose Low LET Ionizing Radiation Exposure: Pathways and Mechanisms Final Report, September 2013 Rocke,...

  10. The skin's role in human thermoregulation and comfort

    E-Print Network [OSTI]

    Arens, Edward A; Zhang, H.

    2006-01-01

    the convective and radiation heat transfer are about equal (heat transfer at the skin surface (via conduction, convection, and radiation (Heat transfer through and above the skin. outdoors, wind strongly affects convective heat loss or gain, and radiation (

  11. Skin strain analysis software for the study of human skin deformation

    E-Print Network [OSTI]

    Marecki, Andrew T. (Andrew Thomas)

    2012-01-01

    Skin strain studies have never been conducted in a precise and automated fashion. Previous in vivo strain investigations have been labor intensive and the data resolution was extremely limited such that their results were ...

  12. Predicting Tissue-Specific Enhancers in the Human Genome

    SciTech Connect (OSTI)

    Pennacchio, Len A.; Loots, Gabriela G.; Nobrega, Marcelo A.; Ovcharenko, Ivan

    2006-07-01

    Determining how transcriptional regulatory signals areencoded in vertebrate genomes is essential for understanding the originsof multi-cellular complexity; yet the genetic code of vertebrate generegulation remains poorly understood. In an attempt to elucidate thiscode, we synergistically combined genome-wide gene expression profiling,vertebrate genome comparisons, and transcription factor binding siteanalysis to define sequence signatures characteristic of candidatetissue-specific enhancers in the human genome. We applied this strategyto microarray-based gene expression profiles from 79 human tissues andidentified 7,187 candidate enhancers that defined their flanking geneexpression, the majority of which were located outside of knownpromoters. We cross-validated this method for its ability to de novopredict tissue-specific gene expression and confirmed its reliability in57 of the 79 available human tissues, with an average precision inenhancer recognition ranging from 32 percent to 63 percent, and asensitivity of 47 percent. We used the sequence signatures identified bythis approach to assign tissue-specific predictions to ~;328,000human-mouse conserved noncoding elements in the human genome. Byoverlapping these genome-wide predictions with a large in vivo dataset ofenhancers validated in transgenic mice, we confirmed our results with a28 percent sensitivity and 50 percent precision. These results indicatethe power of combining complementary genomic datasets as an initialcomputational foray into the global view of tissue-specific generegulation in vertebrates.

  13. Determination of refractive indices of porcine skin tissues and Intralipid at eight wavelengths

    E-Print Network [OSTI]

    .3030, 170.7050. 1. INTRODUCTION Boundaries of a homogeneous biological tissue or inter- faces within

  14. Development and Investigation of Synthetic Skin Simulant Platform (3SP) in Friction Blister Applications

    E-Print Network [OSTI]

    Guerra, Carlos

    2012-02-14

    Skin is the largest organ of the human body. It is the first line of defense between the vulnerable organs and tissues of the body and the environment. Healthy skin is paramount to avoiding infection and disease. Therefore, ...

  15. Continuous Wave Terahertz Reflection Imaging of Human Colorectal Tissue

    E-Print Network [OSTI]

    Massachusetts at Lowell, University of

    Continuous Wave Terahertz Reflection Imaging of Human Colorectal Tissue Pallavi Doradlaa,b , Karim cooled silicon bolometer detector. Using polarizers in the experiment both co-polarized and cross the microwave and infrared regions of the spectrum. The high sensitivity of THz radiation to water concentration

  16. Sensitive method for measurement of telomeric DNA content in human tissues

    DOE Patents [OSTI]

    Bryant, Jennifer E. (Albuquerque, NM); Hutchings, Kent G. (Albuquerque, NM); Moyzis, Robert K. (Corona Del Mar, CA); Griffith, Jeffrey K. (Cedar Crest, NM)

    1999-02-16

    A sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue.

  17. Thermal analysis of ultrathin, compliant sensors for characterization of the human skin

    E-Print Network [OSTI]

    Rogers, John A.

    and identifying optimized designs. This paper presents an analytical model, validated by the finite element method that have the ability to bend, twist, and stretch like human skin, with minimal effect on natural processes onto a thin, low elastic modulus (60 mm thick, 30 kPa) elastomeric sheet. A cross-sectional view

  18. EVALUATION OF SUB-ZERO AND RESIDENCE TIMES AFTER CONTINUOUS VERSUS MULTIPLE INTERMITTENT CRYOGEN SPRAY COOLING EXPOSURE ON HUMAN SKIN

    E-Print Network [OSTI]

    Aguilar, Guillermo

    ; (2) high heat transfer rates as cryogen is deposited onto the skin and evaporates at the sprayed SPRAY COOLING EXPOSURE ON HUMAN SKIN PHANTOM Julio C. Ramirez-San-Juan Beckman Laser Institute Guillermo Aguilar Department of Mechanical Engineering, University of California Riverside ABSTRACT Cryogen

  19. Enhanced human papillomavirus type 8 oncogene expression levels are crucial for skin tumorigenesis in transgenic mice

    SciTech Connect (OSTI)

    Hufbauer, M.; Lazic, D.; Akguel, B.; Brandsma, J.L.; Pfister, H.; Weissenborn, S.J.

    2010-08-01

    Human papillomavirus 8 (HPV8) is involved in skin cancer development in epidermodysplasia verruciformis patients. Transgenic mice expressing HPV8 early genes (HPV8-CER) developed papillomas, dysplasias and squamous cell carcinomas. UVA/B-irradiation and mechanical wounding of HPV8-CER mouse skin led to prompt papilloma induction in about 3 weeks. The aim of this study was to analyze the kinetics and level of transgene expression in response to skin irritations. Transgene expression was already enhanced 1 to 2 days after UVA/B-irradiation or tape-stripping and maintained during papilloma development. The enhanced transgene expression could be assigned to UVB and not to UVA. Papilloma development was thus always paralleled by an increased transgene expression irrespective of the type of skin irritation. A knock-down of E6 mRNA by tattooing HPV8-E6-specific siRNA led to a delay and a lower incidence of papilloma development. This indicates that the early increase of viral oncogene expression is crucial for induction of papillomatosis.

  20. High and Low Doses of Ionizing Radiation Induce Different Secretome Profiles in a Human Skin Model

    SciTech Connect (OSTI)

    Zhang, Qibin; Matzke, Melissa M.; Schepmoes, Athena A.; Moore, Ronald J.; Webb-Robertson, Bobbie-Jo M.; Hu, Zeping; Monroe, Matthew E.; Qian, Weijun; Smith, Richard D.; Morgan, William F.

    2014-03-18

    It is postulated that secreted soluble factors are important contributors of bystander effect and adaptive responses observed in low dose ionizing radiation. Using multidimensional liquid chromatography-mass spectrometry based proteomics, we quantified the changes of skin tissue secretome the proteins secreted from a full thickness, reconstituted 3-dimensional skin tissue model 48 hr after exposure to 3, 10 and 200 cGy of X-rays. Overall, 135 proteins showed statistical significant difference between the sham (0 cGy) and any of the irradiated groups (3, 10 or 200 cGy) on the basis of Dunnett adjusted t-test; among these, 97 proteins showed a trend of downregulation and 9 proteins showed a trend of upregulation with increasing radiation dose. In addition, there were 21 and 8 proteins observed to have irregular trends with the 10 cGy irradiated group either having the highest or the lowest level among all three radiated doses. Moreover, two proteins, carboxypeptidase E and ubiquitin carboxyl-terminal hydrolase isozyme L1 were sensitive to ionizing radiation, but relatively independent of radiation dose. Conversely, proteasome activator complex subunit 2 protein appeared to be sensitive to the dose of radiation, as rapid upregulation of this protein was observed when radiation doses were increased from 3, to 10 or 200 cGy. These results suggest that different mechanisms of action exist at the secretome level for low and high doses of ionizing radiation.

  1. Sensitive method for measurement of telomeric DNA content in human tissues

    DOE Patents [OSTI]

    Bryant, J.E.; Hutchings, K.G.; Moyzis, R.K.; Griffith, J.K.

    1999-02-16

    This research discloses a sensitive method for measurement of telomeric DNA content in human tissue, based upon the ratio of telomeric to centromeric DNA present in the tissue. 5 figs.

  2. Approaches to in vitro tissue regeneration with application for human disease modeling and drug development

    E-Print Network [OSTI]

    Young, Carissa L.

    Reliable in vitro human disease models that capture the complexity of in vivo tissue behaviors are crucial to gain mechanistic insights into human disease and enable the development of treatments that are effective across ...

  3. Online quantitative analysis of multispectral images of human body tissues

    SciTech Connect (OSTI)

    Lisenko, S A [Belarusian State University, Minsk (Belarus)

    2013-08-31

    A method is developed for online monitoring of structural and morphological parameters of biological tissues (haemoglobin concentration, degree of blood oxygenation, average diameter of capillaries and the parameter characterising the average size of tissue scatterers), which involves multispectral tissue imaging, image normalisation to one of its spectral layers and determination of unknown parameters based on their stable regression relation with the spectral characteristics of the normalised image. Regression is obtained by simulating numerically the diffuse reflectance spectrum of the tissue by the Monte Carlo method at a wide variation of model parameters. The correctness of the model calculations is confirmed by the good agreement with the experimental data. The error of the method is estimated under conditions of general variability of structural and morphological parameters of the tissue. The method developed is compared with the traditional methods of interpretation of multispectral images of biological tissues, based on the solution of the inverse problem for each pixel of the image in the approximation of different analytical models. (biomedical optics)

  4. Development of a method for assessing non-targeted radiation damage in an artificial 3D human skin model

    E-Print Network [OSTI]

    Brenner, David Jonathan

    at doses as low as 0.1 Gy in the directly irradiated as well as in the bystander cells. Conclusions. #12;critical relevance in low-dose and/or non-Development of a method for assessing non-targeted radiation damage in an artificial 3D human skin

  5. Photoreactivation in bacteria and in skin

    SciTech Connect (OSTI)

    Sutherland, B M

    1980-01-01

    In many procaryotic and eucaryotic cells, photoreactivating enzyme mediates light-dependent repair of uv-induced damage; the enzyme binds to a pyrimidine dimer in DNA, and, on absorption of a photon (300 to 600 nm), specifically monomerizes the dimer, thus repairing the DNA. Photoreactivating enzyme has been found in human tissues and human cells in culture can photoreactivate cellular dimers, and can mediate photoreactivation of Herpes (human fibroblasts) and Epstein-Barr virus (human leukocytes). Measurements of pyrimidine dimer formation and repair in human skin indicate that detectable numbers of dimers are formed at 1 minimal erythemal dose, that the dimiers are rapidly removed in skin kept in the absence of light, and they are more rapidly removed when the skin is exposed to visible light. Whether this apparent photorecovery is true, enzymatic photoreactivation is yet to be determined.

  6. S.N.A.K.E. : a dynamically reconfigurable Artificial Sensate Skin

    E-Print Network [OSTI]

    Barroeta Prez, Gerardo

    2006-01-01

    The idea of an Artificial Sensate Skin device that mimics the characteristics and functions of its analogous living tissue whether human or animal is not new. Yet, most of the current related work has been focused in the ...

  7. Reflective Terahertz Imaging for early diagnosis of skin burn severity

    E-Print Network [OSTI]

    TEWARI, PRIYAMVADA

    2013-01-01

    of human skin was used for hydration experiments whereby itthe human skin, was used for the hydration experiments and

  8. Mineral density volume gradients in normal and diseased human tissues

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Djomehri, Sabra I.; Candell, Susan; Case, Thomas; Browning, Alyssa; Marshall, Grayson W.; Yun, Wenbing; Lau, S. H.; Webb, Samuel; Ho, Sunita P.; Aikawa, Elena

    2015-04-09

    Clinical computed tomography provides a single mineral density (MD) value for heterogeneous calcified tissues containing early and late stage pathologic formations. The novel aspect of this study is that, it extends current quantitative methods of mapping mineral density gradients to three dimensions, discretizes early and late mineralized stages, identifies elemental distribution in discretized volumes, and correlates measured MD with respective calcium (Ca) to phosphorus (P) and Ca to zinc (Zn) elemental ratios. To accomplish this, MD variations identified using polychromatic radiation from a high resolution micro-computed tomography (micro-CT) benchtop unit were correlated with elemental mapping obtained from a microprobe X-raymorefluorescence (XRF) using synchrotron monochromatic radiation. Digital segmentation of tomograms from normal and diseased tissues (N=5 per group; 40-60 year old males) contained significant mineral density variations (enamel: 2820-3095mg/cc, bone: 570-1415mg/cc, cementum: 1240-1340mg/cc, dentin: 1480-1590mg/cc, cementum affected by periodontitis: 1100-1220mg/cc, hypomineralized carious dentin: 345-1450mg/cc, hypermineralized carious dentin: 1815-2740mg/cc, and dental calculus: 1290-1770mg/cc). A plausible linear correlation between segmented MD volumes and elemental ratios within these volumes was established, and Ca/P ratios for dentin (1.49), hypomineralized dentin (0.32-0.46), cementum (1.51), and bone (1.68) were observed. Furthermore, varying Ca/Zn ratios were distinguished in adapted compared to normal tissues, such as in bone (855-2765) and in cementum (595-990), highlighting Zn as an influential element in prompting observed adaptive properties. Hence, results provide insights on mineral density gradients with elemental concentrations and elemental footprints that in turn could aid in elucidating mechanistic processes for pathologic formations.less

  9. Photochemical tissue bonding

    DOE Patents [OSTI]

    Redmond, Robert W. (Brookline, MA); Kochevar, Irene E. (Charlestown, MA)

    2012-01-10

    Photochemical tissue bonding methods include the application of a photosensitizer to a tissue and/or tissue graft, followed by irradiation with electromagnetic energy to produce a tissue seal. The methods are useful for tissue adhesion, such as in wound closure, tissue grafting, skin grafting, musculoskeletal tissue repair, ligament or tendon repair and corneal repair.

  10. p53 modulates the AMPK inhibitor compound C induced apoptosis in human skin cancer cells

    SciTech Connect (OSTI)

    Huang, Shi-Wei [Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan (China); Wu, Chun-Ying [Division of Gastroenterology and Hepatology, Taichung Veterans General Hospital, Taichung, Taiwan (China); Wang, Yen-Ting [Department of Medical Research and Education, Cheng Hsin General Hospital, Taipei, Taiwan (China); Kao, Jun-Kai [Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan (China); Department of Pediatrics, Children's Hospital, Changhua Christian Hospital, Changhua, Taiwan (China); Lin, Chi-Chen; Chang, Chia-Che; Mu, Szu-Wei; Chen, Yu-Yu [Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan (China); Chiu, Husan-Wen [Institute of Biotechnology, National Cheng-Kung University, Tainan, Taiwan (China); Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan (China); Chang, Chuan-Hsun [Department of Surgical Oncology, Cheng Hsin General Hospital, Taipei, Taiwan (China); Department of Nutrition Therapy, Cheng Hsin General Hospital, Taipei, Taiwan (China); School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan (China); Liang, Shu-Mei [Institute of Biotechnology, National Cheng-Kung University, Tainan, Taiwan (China); Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan (China); Chen, Yi-Ju [Department of Dermatology, Taichung Veterans General Hospital, Taichung, Taiwan (China); Huang, Jau-Ling [Department of Bioscience Technology, Chang Jung Christian University, Tainan, Taiwan (China); Shieh, Jeng-Jer, E-mail: shiehjj@vghtc.gov.tw [Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan (China); Department of Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan (China)

    2013-02-15

    Compound C, a well-known inhibitor of the intracellular energy sensor AMP-activated protein kinase (AMPK), has been reported to cause apoptotic cell death in myeloma, breast cancer cells and glioma cells. In this study, we have demonstrated that compound C not only induced autophagy in all tested skin cancer cell lines but also caused more apoptosis in p53 wildtype skin cancer cells than in p53-mutant skin cancer cells. Compound C can induce upregulation, phosphorylation and nuclear translocalization of the p53 protein and upregulate expression of p53 target genes in wildtype p53-expressing skin basal cell carcinoma (BCC) cells. The changes of p53 status were dependent on DNA damage which was caused by compound C induced reactive oxygen species (ROS) generation and associated with activated ataxia-telangiectasia mutated (ATM) protein. Using the wildtype p53-expressing BCC cells versus stable p53-knockdown BCC sublines, we present evidence that p53-knockdown cancer cells were much less sensitive to compound C treatment with significant G2/M cell cycle arrest and attenuated the compound C-induced apoptosis but not autophagy. The compound C induced G2/M arrest in p53-knockdown BCC cells was associated with the sustained inactive Tyr15 phosphor-Cdc2 expression. Overall, our results established that compound C-induced apoptosis in skin cancer cells was dependent on the cell's p53 status. - Highlights: ? Compound C caused more apoptosis in p53 wildtype than p53-mutant skin cancer cells. ? Compound C can upregulate p53 expression and induce p53 activation. ? Compound C induced p53 effects were dependent on ROS induced DNA damage pathway. ? p53-knockdown attenuated compound C-induced apoptosis but not autophagy. ? Compound C-induced apoptosis in skin cancer cells was dependent on p53 status.

  11. Thermal Modeling and Experimental Validation of Human Hair and Skin Heated by Broadband Light

    E-Print Network [OSTI]

    Aguilar, Guillermo

    distribution within the hair follicle is highly non-uniform: the minimum temperature occurs at the follicle Sun, PhD,1 Alex Chaney,1 Robert Anderson, PhD,2 and Guillermo Aguilar, PhD 1 * 1 Department:(a)determinetheoveralleffectofPPxonskinhumidi- tyandassociatedskinopticalproperties,and;(b)developaPT numerical model to study the spatial and temporal hair and skin temperature

  12. Green tea polyphenol, (?)-epigallocatechin-3-gallate, induces toxicity in human skin cancer cells by targeting ?-catenin signaling

    SciTech Connect (OSTI)

    Singh, Tripti; Katiyar, Santosh K.

    2013-12-01

    The green tea polyphenol, (?)-epigallocatechin-3-gallate (EGCG), has been shown to have anti-carcinogenic effects in several skin tumor models, and efforts are continued to investigate the molecular targets responsible for its cytotoxic effects to cancer cells. Our recent observation that ?-catenin is upregulated in skin tumors suggested the possibility that the anti-skin carcinogenic effects of EGCG are mediated, at least in part, through its effects on ?-catenin signaling. We have found that treatment of the A431 and SCC13 human skin cancer cell lines with EGCG resulted in reduced cell viability and increased cell death and that these cytotoxic effects were associated with inactivation of ?-catenin signaling. Evidence of EGCG-induced inactivation of ?-catenin included: (i) reduced accumulation of nuclear ?-catenin; (ii) enhanced levels of casein kinase1?, reduced phosphorylation of glycogen synthase kinase-3?, and increased phosphorylation of ?-catenin on critical serine{sup 45,33/37} residues; and (iii) reduced levels of matrix metalloproteinase (MMP)-2 and MMP-9, which are down-stream targets of ?-catenin. Treatment of cells with prostaglandin E2 (PGE{sub 2}) enhanced the accumulation of ?-catenin and enhanced ?-catenin signaling. Treatment with either EGCG or an EP2 antagonist (AH6809) reduced the PGE{sub 2}-enhanced levels of cAMP, an upstream regulator of ?-catenin. Inactivation of ?-catenin by EGCG resulted in suppression of cell survival signaling proteins. siRNA knockdown of ?-catenin in A431 and SCC13 cells reduced cell viability. Collectively, these data suggest that induction of cytotoxicity in skin cancer cells by EGCG is mediated by targeting of ?-catenin signaling and that the ?-catenin signaling is upregulated by inflammatory mediators. - Highlights: EGCG inhibits cancer cell viability through inactivation of ?-catenin signaling. Inactivation of ?-catenin involves the downregulation of inflammatory mediators. EGCG inactivates ?-catenin in skin cancer cells by inhibition of cAMP and PGE{sub 2}. siRNA knockdown of ?-catenin or COX-2 reduces the viability of cancer cells.

  13. Changes in Womens Facial Skin Color Over the Ovulatory Cycle are Not Detectable by the Human Visual System

    E-Print Network [OSTI]

    Burriss, Robert P.; Troscianko, Jolyon; Lovell, P. George; Fulford, Anthony J. C.; Stevens, Martin; Quigley, Rachael; Payne, Jenny; Saxton, Tamsin K.; Rowland, Hannah M.

    2015-01-01

    the mean RGB values for each patch, and converted these to photon 197 catch values equivalent to long, medium, and short wave (LMS) cone responses, and to CIE 198 XYZ responses. We then averaged the left and right patch values, giving one color value per... 199 photograph. Cone-catch models were generated following the methodology of Prraga et al. 200 Running head: FACIAL SKIN COLOR AND THE OVULATORY CYCLE 11 [59]. Human cone-catch quanta (LMS sensitivities from Stockman and Sharpe [71], and CIE 201...

  14. A hybrid approach to advancing quantitative prediction of tissue distribution of basic drugs in human

    SciTech Connect (OSTI)

    Poulin, Patrick, E-mail: patrick-poulin@videotron.ca [Quebec City, Quebec (Canada); Ekins, Sean [Collaborations in Chemistry, 601 Runnymede Avenue, Jenkintown, PA 19046 (United States); Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, 20 Penn Street, Baltimore, MD 21201 (United States); Department of Pharmacology, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854 (United States); Theil, Frank-Peter [Genentech, South San Francisco (United States)

    2011-01-15

    A general toxicity of basic drugs is related to phospholipidosis in tissues. Therefore, it is essential to predict the tissue distribution of basic drugs to facilitate an initial estimate of that toxicity. The objective of the present study was to further assess the original prediction method that consisted of using the binding to red blood cells measured in vitro for the unbound drug (RBCu) as a surrogate for tissue distribution, by correlating it to unbound tissue:plasma partition coefficients (Kpu) of several tissues, and finally to predict volume of distribution at steady-state (V{sub ss}) in humans under in vivo conditions. This correlation method demonstrated inaccurate predictions of V{sub ss} for particular basic drugs that did not follow the original correlation principle. Therefore, the novelty of this study is to provide clarity on the actual hypotheses to identify i) the impact of pharmacological mode of action on the generic correlation of RBCu-Kpu, ii) additional mechanisms of tissue distribution for the outlier drugs, iii) molecular features and properties that differentiate compounds as outliers in the original correlation analysis in order to facilitate its applicability domain alongside the properties already used so far, and finally iv) to present a novel and refined correlation method that is superior to what has been previously published for the prediction of human V{sub ss} of basic drugs. Applying a refined correlation method after identifying outliers would facilitate the prediction of more accurate distribution parameters as key inputs used in physiologically based pharmacokinetic (PBPK) and phospholipidosis models.

  15. On the deformation of human skin for mechanical counter pressure space suit development

    E-Print Network [OSTI]

    Obropta, Edward William, Jr

    2015-01-01

    Exploration of planetary bodies requires space suits that do not inhibit astronaut mobility. Gas pressurized suits are typically bulky and stiff to operate or require unnatural human motion. Development of mechanical counter ...

  16. Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation

    E-Print Network [OSTI]

    Park, Sang-Hyug

    Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs ...

  17. Sources of Technical Variability in Quantitative LC-MS Proteomics: Human Brain Tissue Sample Analysis.

    SciTech Connect (OSTI)

    Piehowski, Paul D.; Petyuk, Vladislav A.; Orton, Daniel J.; Xie, Fang; Moore, Ronald J.; Ramirez Restrepo, Manuel; Engel, Anzhelika; Lieberman, Andrew P.; Albin, Roger L.; Camp, David G.; Smith, Richard D.; Myers, Amanda J.

    2013-05-03

    To design a robust quantitative proteomics study, an understanding of both the inherent heterogeneity of the biological samples being studied as well as the technical variability of the proteomics methods and platform is needed. Additionally, accurately identifying the technical steps associated with the largest variability would provide valuable information for the improvement and design of future processing pipelines. We present an experimental strategy that allows for a detailed examination of the variability of the quantitative LC-MS proteomics measurements. By replicating analyses at different stages of processing, various technical components can be estimated and their individual contribution to technical variability can be dissected. This design can be easily adapted to other quantitative proteomics pipelines. Herein, we applied this methodology to our label-free workflow for the processing of human brain tissue. For this application, the pipeline was divided into four critical components: Tissue dissection and homogenization (extraction), protein denaturation followed by trypsin digestion and SPE clean-up (digestion), short-term run-to-run instrumental response fluctuation (instrumental variance), and long-term drift of the quantitative response of the LC-MS/MS platform over the 2 week period of continuous analysis (instrumental stability). From this analysis, we found the following contributions to variability: extraction (72%) >> instrumental variance (16%) > instrumental stability (8.4%) > digestion (3.1%). Furthermore, the stability of the platform and its suitability for discovery proteomics studies is demonstrated.

  18. Skin flicks

    E-Print Network [OSTI]

    Orth, Margaret A. (Margaret Ann), 1964-

    1993-01-01

    The written and artistic part of this thesis are both separated into the two categories of "SKIN" and "FLICKS". The Artistic part of my thesis consists of five artificial skins made on my body, and a series of video tapes ...

  19. Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease

    E-Print Network [OSTI]

    Saha, Krishanu

    Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients ...

  20. Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra

    E-Print Network [OSTI]

    Laiho, Lily H.

    Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy ...

  1. Skin contamination dosimeter

    DOE Patents [OSTI]

    Hamby, David M. (Corvallis, OR); Farsoni, Abdollah T. (Corvallis, OR); Cazalas, Edward (Corvallis, OR)

    2011-06-21

    A technique and device provides absolute skin dosimetry in real time at multiple tissue depths simultaneously. The device uses a phoswich detector which has multiple scintillators embedded at different depths within a non-scintillating material. A digital pulse processor connected to the phoswich detector measures a differential distribution (dN/dH) of count rate N as function of pulse height H for signals from each of the multiple scintillators. A digital processor computes in real time from the differential count-rate distribution for each of multiple scintillators an estimate of an ionizing radiation dose delivered to each of multiple depths of skin tissue corresponding to the multiple scintillators embedded at multiple corresponding depths within the non-scintillating material.

  2. An investigation of the structure-function relationship in human cervical tissue

    E-Print Network [OSTI]

    Myers, Kristin M

    2008-01-01

    The cervix plays a crucial role in maintaining a healthy pregnancy, acting as a mechanical barrier to hold the fetus inside the uterus during gestation. Altered biochemical and mechanical properties of the cervical tissue ...

  3. Healthy Skin Matters Normal Skin

    E-Print Network [OSTI]

    Rau, Don C.

    into contact with the rest of the world. It holds body fluids in, preventing dehydration (dee when your skin is injured If you do get a cut or scratch, clean it right away with soap and warm water

  4. ARTHROPOD/HOST INTERACTION, IMMUNITY Gene Expression and Tissue Distribution of the Major Human

    E-Print Network [OSTI]

    to cockroach allergens is an important risk factor for allergic disease in humans. Despite a recent burgeoning of clinical and socioeconomic studies regarding environmental pervasiveness and human exposure to cockroach THE GERMAN COCKROACH, Blattella germanica L., is a synanthropic pest of considerable economic, veteri- nary

  5. Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue

    E-Print Network [OSTI]

    Ma, Jiao

    The existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection of their sORF-encoded polypeptide (SEP) products. The discovery of novel SEPs increases ...

  6. Oral administration of drugs with hypersensitivity potential induces germinal center hyperplasia in secondary lymphoid organ/tissue in Brown Norway rats, and this histological lesion is a promising candidate as a predictive biomarker for drug hypersensitivity occurrence in humans

    SciTech Connect (OSTI)

    Tamura, Akitoshi, E-mail: akitoshi-tamura@ds-pharma.co.jp; Miyawaki, Izuru; Yamada, Toru; Kimura, Juki; Funabashi, Hitoshi

    2013-08-15

    It is important to evaluate the potential of drug hypersensitivity as well as other adverse effects during the preclinical stage of the drug development process, but validated methods are not available yet. In the present study we examined whether it would be possible to develop a new predictive model of drug hypersensitivity using Brown Norway (BN) rats. As representative drugs with hypersensitivity potential in humans, phenytoin (PHT), carbamazepine (CBZ), amoxicillin (AMX), and sulfamethoxazole (SMX) were orally administered to BN rats for 28 days to investigate their effects on these animals by examinations including observation of clinical signs, hematology, determination of serum IgE levels, histology, and flow cytometric analysis. Skin rashes were not observed in any animals treated with these drugs. Increases in the number of circulating inflammatory cells and serum IgE level did not necessarily occur in the animals treated with these drugs. However, histological examination revealed that germinal center hyperplasia was commonly induced in secondary lymphoid organs/tissues in the animals treated with these drugs. In cytometric analysis, changes in proportions of lymphocyte subsets were noted in the spleen of the animals treated with PHT or CBZ during the early period of administration. The results indicated that the potential of drug hypersensitivity was identified in BN rat by performing histological examination of secondary lymphoid organs/tissues. Data obtained herein suggested that drugs with hypersensitivity potential in humans gained immune reactivity in BN rat, and the germinal center hyperplasia induced by administration of these drugs may serve as a predictive biomarker for drug hypersensitivity occurrence. - Highlights: We tested Brown Norway rats as a candidate model for predicting drug hypersensitivity. The allergic drugs did not induce skin rash, whereas D-penicillamine did so in the rats. Some of allergic drugs increased inflammatory cells and IgE, but the others did not. The allergic drugs commonly induced germinal center hyperplasia in lymphoid tissues. Some of these allergic drugs transiently increased CD4{sup +}CD25{sup +} T cells in the spleen.

  7. National Human Radiobiological Tissue Repository (NHRTR) at the United States Transuranium and Uranium Registries

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    The NHRTR, one component of the USTUR, contains frozen tissues, tissue solutions, microscope slides, and paraffin blocks that were collected by the USTUR at the autopsy of workers with documented intakes of plutonium, americium, uranium, and thorium. The samples are available to qualified scientists for further research. Thousands of frozen, ashed, dried, and plastic embedded bone samples from the radium studies carried out by Argonne National Laboratory, Argonne Cancer Research Hospital, the Massachusetts Institute of Technology, and the New Jersey Radium Research Project are available and linked by case number to de-identified, published case data. These data include the person's source of exposure (dial painter, therapeutic injection, etc.), estimated body burden, radiochemical results, and medical history. Other samples, including organs and whole body donations, have come from volunteer donors who were impacted by elements such as plutonium, throium, etc. See the USTUR website for information on how to apply for research samples or how to become a volunteer donor. [Information taken from http://www.ustur.wsu.edu/NHRTR/index.html#

  8. Transgenic rats overexpressing the human MrgX3 gene show cataracts and an abnormal skin phenotype

    SciTech Connect (OSTI)

    Kaisho, Yoshihiko . E-mail: Kaisho_Yoshihiko@takeda.co.jp; Watanabe, Takuya; Nakata, Mitsugu; Yano, Takashi; Yasuhara, Yoshitaka; Shimakawa, Kozo; Mori, Ikuo; Sakura, Yasufumi; Terao, Yasuko; Matsui, Hideki; Taketomi, Shigehisa

    2005-05-13

    The human MrgX3 gene, belonging to the mrgs/SNSRs (mass related genes/sensory neuron specific receptors) family, was overexpressed in transgenic rats using the actin promoter. Two animal lines showed cataracts with liquification/degeneration and swelling of the lens fiber cells. The transient epidermal desquamation was observed in line with higher gene expression. Histopathology of the transgenic rats showed acanthosis and focal parakeratosis. In the epidermis, there was an increase in cellular keratin 14, keratin 10, and loricrin, as well as PGP 9.5 in innervating nerve fibers. These phenotypes accompanied an increase in the number of proliferating cells. These results suggest that overexpression of the human MrgX3 gene causes a disturbance of the normal cell-differentiation process.

  9. The Skin Microbiome in Healthy and Allergic Dogs

    E-Print Network [OSTI]

    Hoffmann, Aline Rodrigues; Patterson, Adam P.; Diesel, Alison; Lawhon, Sara D.; Ly, Hoai Jaclyn; Stephenson, Christine Elkins; Mansell, Joanne; Steiner, Jö rg M.; Dowd, Scot E.; Olivry, Thierry; Suchodolski, Jan S.

    2014-01-08

    Changes in the microbial populations on the skin of animals have traditionally been evaluated using conventional microbiology techniques. The sequencing of bacterial 16S rRNA genes has revealed that the human skin is inhabited by a highly diverse...

  10. Skin Bleaching in Jamaica: A Colonial Legacy

    E-Print Network [OSTI]

    Robinson, Petra Alaine

    2012-07-16

    -1 SKIN BLEACHING IN JAMAICA: A COLONIAL LEGACY A Dissertation by PETRA ALAINE ROBINSON Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY... May 2011 Major Subject: Educational Human Resource Development Skin Bleaching in Jamaica: A Colonial Legacy Copyright 2011 Petra Alaine Robinson SKIN BLEACHING IN JAMAICA: A COLONIAL LEGACY...

  11. Effects of FGF-2 on human adipose tissue derived adult stem cells morphology and chondrogenesis enhancement in Transwell culture

    SciTech Connect (OSTI)

    Kabiri, Azadeh; Esfandiari, Ebrahim; Hashemibeni, Batool; Kazemi, Mohammad; Mardani, Mohammad; Esmaeili, Abolghasem

    2012-07-27

    Highlights: Black-Right-Pointing-Pointer We investigated effects of FGF-2 on hADSCs. Black-Right-Pointing-Pointer We examine changes in the level of gene expressions of SOX-9, aggrecan and collagen type II and type X. Black-Right-Pointing-Pointer FGF-2 induces chondrogenesis in hADSCs, which Bullet Increasing information will decrease quality if hospital costs are very different. Black-Right-Pointing-Pointer The result of this study may be beneficial in cartilage tissue engineering. -- Abstract: Injured cartilage is difficult to repair due to its poor vascularisation. Cell based therapies may serve as tools to more effectively regenerate defective cartilage. Both adult mesenchymal stem cells (MSCs) and human adipose derived stem cells (hADSCs) are regarded as potential stem cell sources able to generate functional cartilage for cell transplantation. Growth factors, in particular the TGF-b superfamily, influence many processes during cartilage formation, including cell proliferation, extracellular matrix synthesis, maintenance of the differentiated phenotype, and induction of MSCs towards chondrogenesis. In the current study, we investigated the effects of FGF-2 on hADSC morphology and chondrogenesis in Transwell culture. hADSCs were obtained from patients undergoing elective surgery, and then cultured in expansion medium alone or in the presence of FGF-2 (10 ng/ml). mRNA expression levels of SOX-9, aggrecan and collagen type II and type X were quantified by real-time polymerase chain reaction. The morphology, doubling time, trypsinization time and chondrogenesis of hADSCs were also studied. Expression levels of SOX-9, collagen type II, and aggrecan were all significantly increased in hADSCs expanded in presence of FGF-2. Furthermore FGF-2 induced a slender morphology, whereas doubling time and trypsinization time decreased. Our results suggest that FGF-2 induces hADSCs chondrogenesis in Transwell culture, which may be beneficial in cartilage tissue engineering.

  12. Depth-resolved monitoring of diffusion of hyperosmotic agents in normal and malignant human esophagus tissues using optical coherence tomography in-vitro

    SciTech Connect (OSTI)

    Zhao Qingliang; Guo Zhouyi; Wei Huajiang; Yang Hongqin; Xie Shusen

    2011-10-31

    Depth-resolved monitoring with differentiation and quantification of glucose diffusion in healthy and abnormal esophagus tissues has been studied in vitro. Experiments have been performed using human normal esophagus and esophageal squamous cell carcinoma (ESCC) tissues by the optical coherence tomography (OCT). The images have been continuously acquired for 120 min in the experiments, and the depth-resolved and average permeability coefficients of the 40 % glucose solution have been calculated by the OCT amplitude (OCTA) method. We demonstrate the capability of the OCT technique for depth-resolved monitoring, differentiation, and quantifying of glucose diffusion in normal esophagus and ESCC tissues. It is found that the permeability coefficients of the 40 % glucose solution are not uniform throughout the normal esophagus and ESCC tissues and increase from (3.30 {+-} 0.09) Multiplication-Sign 10{sup -6} and (1.57 {+-} 0.05) Multiplication-Sign 10{sup -5} cm s{sup -1} at the mucous membrane of normal esophagus and ESCC tissues to (1.82 {+-} 0.04) Multiplication-Sign 10{sup -5} and (3.53 {+-} 0.09) Multiplication-Sign 10{sup -5} cm s{sup -1} at the submucous layer approximately 742 {mu}m away from the epithelial surface of normal esophagus and ESCC tissues, respectively. (optical coherence tomography)

  13. Chemoprevention of human skin cancers.

    E-Print Network [OSTI]

    Loescher, L J; Meyskens, F L Jr

    1991-01-01

    activity of celecoxib, a specific cyclooxygenase- 2ad- ministration of celecoxib, a selective COX-2 inhibitor,

  14. Chemoprevention of human skin cancers.

    E-Print Network [OSTI]

    Loescher, L J; Meyskens, F L Jr

    1991-01-01

    chemopreventive activity of resveratrol, a natural productJM, Dannenberg AJ. Resveratrol inhibits cyclooxygenase-2C, Ma WY, Goranson A, Dong Z. Resveratrol sup- presses cell

  15. CXCR6, a Newly Defined Biomarker of Tissue-Specific Stem Cell Asymmetric Self-Renewal, Identifies More Aggressive Human Melanoma Cancer Stem Cells

    E-Print Network [OSTI]

    Rouzbeh Taghizadeh; Minsoo Noh; Yang Hoon Huh; Emilio Ciusani; Luca Sigalotti; Michele Maio; Beatrice Arosio; Maria R. Nicotra; PierGiorgio Natali; James L. Sherley; Caterina A. M. La Porta

    2013-08-28

    Background: A fundamental problem in cancer research is identifying the cell type that is capable of sustaining neoplastic growth and its origin from normal tissue cells. Recent investigations of a variety of tumor types have shown that phenotypically identifiable and isolable subfractions of cells possess the tumor-forming ability. In the present paper, using two lineage-related human melanoma cell lines, primary melanoma line IGR39 and its metastatic derivative line IGR37, two main observations are reported. The first one is the first phenotypic evidence to support the origin of melanoma cancer stem cells (CSCs) from mutated tissue-specific stem cells; and the second one is the identification of a more aggressive subpopulation of CSCs in melanoma that are CXCR6+. Conclusions/Significance: The association of a more aggressive tumor phenotype with asymmetric self-renewal phenotype reveals a previously unrecognized aspect of tumor cell physiology. Namely, the retention of some tissue-specific stem cell attributes, like the ability to asymmetrically self-renew, impacts the natural history of human tumor development. Knowledge of this new aspect of tumor development and progression may provide new targets for cancer prevention and treatment.

  16. Development of a combined model of tissue kinetics and radiation response of human bronchiolar epithelium with single cell resolution

    E-Print Network [OSTI]

    Ostrovskaya, Natela Grigoryevna

    2006-10-30

    cells of the airways due to internal exposure to alpha-particle emitters, e.g. radon. Inhalation of radon, a colorless and odorless gas, one of the products of the decay of uranium which occurs naturally in the earth?s crust, is the second major cause... epithelial tissue plays an important role in normal lung physiology. square4 lung epithelia are target tissues for occupational internal exposures and for radon exposure (26); square4 the epithelium of bronchioles appears to be the origin...

  17. Dosimetric characterization of model Cs-1 Rev2 cesium-131 brachytherapy source in water phantoms and human tissues with MCNP5 Monte Carlo simulation

    SciTech Connect (OSTI)

    Wang Jianhua; Zhang Hualin [Shanghai Institute of Applied Physics, CAS, Shanghai 201800 (China); Department of Radiation Medicine, Ohio State University, Columbus, Ohio 43210 (United States)

    2008-04-15

    A recently developed alternative brachytherapy seed, Cs-1 Rev2 cesium-131, has begun to be used in clinical practice. The dosimetric characteristics of this source in various media, particularly in human tissues, have not been fully evaluated. The aim of this study was to calculate the dosimetric parameters for the Cs-1 Rev2 cesium-131 seed following the recommendations of the AAPM TG-43U1 report [Rivard et al., Med. Phys. 31, 633-674 (2004)] for new sources in brachytherapy applications. Dose rate constants, radial dose functions, and anisotropy functions of the source in water, Virtual Water, and relevant human soft tissues were calculated using MCNP5 Monte Carlo simulations following the TG-43U1 formalism. The results yielded dose rate constants of 1.048, 1.024, 1.041, and 1.044 cGy h{sup -1} U{sup -1} in water, Virtual Water, muscle, and prostate tissue, respectively. The conversion factor for this new source between water and Virtual Water was 1.02, between muscle and water was 1.006, and between prostate and water was 1.004. The authors' calculation of anisotropy functions in a Virtual Water phantom agreed closely with Murphy's measurements [Murphy et al., Med. Phys. 31, 1529-1538 (2004)]. Our calculations of the radial dose function in water and Virtual Water have good agreement with those in previous experimental and Monte Carlo studies. The TG-43U1 parameters for clinical applications in water, muscle, and prostate tissue are presented in this work.

  18. Method and apparatus to measure the depth of skin burns

    DOE Patents [OSTI]

    Dickey, Fred M. (Albuquerque, NM); Holswade, Scott C. (Albuquerque, NM)

    2002-01-01

    A new device for measuring the depth of surface tissue burns based on the rate at which the skin temperature responds to a sudden differential temperature stimulus. This technique can be performed without physical contact with the burned tissue. In one implementation, time-dependent surface temperature data is taken from subsequent frames of a video signal from an infrared-sensitive video camera. When a thermal transient is created, e.g., by turning off a heat lamp directed at the skin surface, the following time-dependent surface temperature data can be used to determine the skin burn depth. Imaging and non-imaging versions of this device can be implemented, thereby enabling laboratory-quality skin burn depth imagers for hospitals as well as hand-held skin burn depth sensors the size of a small pocket flashlight for field use and triage.

  19. DNA Double-Strand Breaks Form in Bystander Cells after Microbeam Irradiation of Three-dimensional Human Tissue Models

    E-Print Network [OSTI]

    Brenner, David Jonathan

    Research Accelerator Facility, Center for Radiological Research, College of Physicians and Surgeons Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada; and 3 Radiological implications for cancer radiother- apy and diagnostic radiology as well as for human health in general

  20. HISTORY OF SKIN GRAFTS and Hauben and colleagues2

    E-Print Network [OSTI]

    Stanford University

    report of successful pinch grafts. Ollier in 1872 pointed out the importance of the dermis in skin grafts, Vandeput, and Olley4 gave us the technology to expand skin grafts with a machine that would cut the graft technology was published by Rheinwald and Green,5 and in 1979 cultured human keratinocytes were grown to form

  1. Mueller matrix imaging for skin cancer detection

    E-Print Network [OSTI]

    Baldwin, Angela Michelle

    2004-09-30

    is suspect, a biopsy is performed to verify the diagnosis. For squamous cell and basal cell carcinomas, the most common treatment options are excision, curettage and electrodesiccation, cryosurgery, radiation, and Moh?s micrographic surgery. Excision... is where all visible cancer is cut away together with a 3 to 10 mm margin of healthy tissue and then the skin is stitched closed with sutures. Curettage and electrodesiccation are where all visible cancer is scraped away and then an electric probe...

  2. Extracellular acidification induces connective tissue growth factor production through proton-sensing receptor OGR1 in human airway smooth muscle cells

    SciTech Connect (OSTI)

    Matsuzaki, Shinichi; Ishizuka, Tamotsu; Yamada, Hidenori; Kamide, Yosuke; Hisada, Takeshi; Ichimonji, Isao; Aoki, Haruka; Yatomi, Masakiyo; Komachi, Mayumi; Tsurumaki, Hiroaki; Ono, Akihiro; Koga, Yasuhiko; Dobashi, Kunio; Mogi, Chihiro; Sato, Koichi; Tomura, Hideaki; Mori, Masatomo; Okajima, Fumikazu

    2011-10-07

    Highlights: {yields} The involvement of extracellular acidification in airway remodeling was investigated. {yields} Extracellular acidification alone induced CTGF production in human ASMCs. {yields} Extracellular acidification enhanced TGF-{beta}-induced CTGF production in human ASMCs. {yields} Proton-sensing receptor OGR1 was involved in acidic pH-stimulated CTGF production. {yields} OGR1 may play an important role in airway remodeling in asthma. -- Abstract: Asthma is characterized by airway inflammation, hyper-responsiveness and remodeling. Extracellular acidification is known to be associated with severe asthma; however, the role of extracellular acidification in airway remodeling remains elusive. In the present study, the effects of acidification on the expression of connective tissue growth factor (CTGF), a critical factor involved in the formation of extracellular matrix proteins and hence airway remodeling, were examined in human airway smooth muscle cells (ASMCs). Acidic pH alone induced a substantial production of CTGF, and enhanced transforming growth factor (TGF)-{beta}-induced CTGF mRNA and protein expression. The extracellular acidic pH-induced effects were inhibited by knockdown of a proton-sensing ovarian cancer G-protein-coupled receptor (OGR1) with its specific small interfering RNA and by addition of the G{sub q/11} protein-specific inhibitor, YM-254890, or the inositol-1,4,5-trisphosphate (IP{sub 3}) receptor antagonist, 2-APB. In conclusion, extracellular acidification induces CTGF production through the OGR1/G{sub q/11} protein and inositol-1,4,5-trisphosphate-induced Ca{sup 2+} mobilization in human ASMCs.

  3. Viability of adult rat skin following 13 Mev proton irradiation

    E-Print Network [OSTI]

    Caraway, Bobby Lamar

    1966-01-01

    alteration resulting from total skin proton irradiation seemed to be dose related. Therefore, since the amount of tissue alteration seems to be dose-dependent, a hypothesis was developed that growth and viability of skin cells removed' by biopsy... rats each were subjected to total-skin proton irradiation of varying doses. The dose varied from 1300 rad in Group I to 200 rad in Group 1V. Two rats from each group served as controls and received no irradiation. Five days and 30 days...

  4. Sprayed skin turbine component

    DOE Patents [OSTI]

    Allen, David B

    2013-06-04

    Fabricating a turbine component (50) by casting a core structure (30), forming an array of pits (24) in an outer surface (32) of the core structure, depositing a transient liquid phase (TLP) material (40) on the outer surface of the core structure, the TLP containing a melting-point depressant, depositing a skin (42) on the outer surface of the core structure over the TLP material, and heating the assembly, thus forming both a diffusion bond and a mechanical interlock between the skin and the core structure. The heating diffuses the melting-point depressant away from the interface. Subsurface cooling channels (35) may be formed by forming grooves (34) in the outer surface of the core structure, filling the grooves with a fugitive filler (36), depositing and bonding the skin (42), then removing the fugitive material.

  5. Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Constructs

    SciTech Connect (OSTI)

    Michael Cornforth

    2012-03-26

    Combining contemporary cytogenetic methods with DNA CGH microarray technology and chromosome flow-sorting increases substantially the ability to resolve exchange breakpoints associated with interstitial deletions and translocations, allowing the consequences of radiation damage to be directly measured at low doses, while also providing valuable insights into molecular mechanisms of misrepair processes that, in turn, identify appropriate biophysical models of risk at low doses. Specific aims apply to cells recovered from 3D tissue constructs of human skin and, for the purpose of comparison, the same cells irradiated in traditional 2D cultures. The project includes research complementary to NASA/HRP space radiation project.

  6. Skin Thermal Injury Prediction with Strain Energy Wensheng Shen y and Jun Zhang z

    E-Print Network [OSTI]

    Zhang, Jun

    Skin Thermal Injury Prediction with Strain Energy #3; Wensheng Shen y and Jun Zhang z Laboratory, in which the activation energy includes chemical reaction only, strain energy of tissue due to thermal-dimensional model is presented for the quantitative prediction of skin injury re- sulting from certain thermal

  7. Master Project in Stem Cell Biology In Vivo Manipulation of Skin Stem Cells

    E-Print Network [OSTI]

    Uppsala Universitet

    Master Project in Stem Cell Biology In Vivo Manipulation of Skin Stem Cells Our lab is interested in understanding how stem cells contribute to tissue homeostasis and disease. Our model system is currently the skin, which harbors several distinct pools of stem cells. In order to identify regulatory networks

  8. In Vivo Biomechanics of the Fingerpad Skin Under Local Tangential Traction

    E-Print Network [OSTI]

    Hayward, Vincent

    In Vivo Biomechanics of the Fingerpad Skin Under Local Tangential Traction Qi Wang and Vincent tested in vivo for their biomechanical properties under tangential loading and for large deforma- tions words: Fingerpad skin properties; In vivo tissue measurement; Biomechanics; Fingers. Word count (main

  9. Effects of ultrasound and sodium lauryl sulfate on the transdermal delivery of hydrophilic permeants: Comparative in vitro studies with full-thickness and split-thickness pig and human skin

    E-Print Network [OSTI]

    Seto, Jennifer E.

    The simultaneous application of ultrasound and the surfactant sodium lauryl sulfate (referred to as US/SLS) to skin enhances transdermal drug delivery (TDD) in a synergistic mechanical and chemical manner. Since full-thickness ...

  10. Enzocide - a chemical dip for the reduction of Salmonella on chicken breast skin

    E-Print Network [OSTI]

    Bianchi, Aldo

    1993-01-01

    /g) in the skin tissue of control-raw (CR,3.350?.47) and waterraw (WR,4.451?.43) were lower than in the water-cooked (WC, 8.911?.94) and Enzocide TM-cooked (EC,7.981?.09) treatments but not Enzocide TM-raw (ER, 5.71?.4). The muscle tissue exhibited no difference...

  11. Ultraviolet-light-induced transformation of human primary cells

    SciTech Connect (OSTI)

    Sutherland, B.M.

    1981-01-01

    The development of model systems for probing the ultraviolet radiation induced oncogenic transformation of human skin cells is described. (ACR)

  12. Oncogenic Radiation Abscopal Effects In Vivo: Interrogating Mouse Skin

    SciTech Connect (OSTI)

    Mancuso, Mariateresa, E-mail: mariateresa.mancuso@enea.it [Laboratory of Radiation Biology and Biomedicine, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Casaccia Research Centre, Rome (Italy); Leonardi, Simona [Laboratory of Radiation Biology and Biomedicine, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Casaccia Research Centre, Rome (Italy); Giardullo, Paola; Pasquali, Emanuela [Department of Radiation Physics, Guglielmo Marconi University, Rome (Italy); Tanori, Mirella [Laboratory of Radiation Biology and Biomedicine, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Casaccia Research Centre, Rome (Italy); De Stefano, Ilaria [Department of Radiation Physics, Guglielmo Marconi University, Rome (Italy); Casciati, Arianna [Laboratory of Radiation Biology and Biomedicine, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Casaccia Research Centre, Rome (Italy); Naus, Christian C. [Department of Cellular and Physiological Sciences, The Life Sciences Institute, University of British Columbia, Vancouver, British Columbia (Canada); Pazzaglia, Simonetta; Saran, Anna [Laboratory of Radiation Biology and Biomedicine, Agenzia Nazionale per le Nuove Tecnologie, l'Energia e lo Sviluppo Economico Sostenibile (ENEA), Casaccia Research Centre, Rome (Italy)

    2013-08-01

    Purpose: To investigate the tissue dependence in transmission of abscopal radiation signals and their oncogenic consequences in a radiosensitive mouse model and to explore the involvement of gap junction intercellular communication (GJIC) in mediating radiation tumorigenesis in off-target mouse skin. Methods and Materials: Patched1 heterozygous (Ptch1{sup +/?}) mice were irradiated at postnatal day 2 (P2) with 10 Gy of x-rays. Individual lead cylinders were used to protect the anterior two-thirds of the body, whereas the hindmost part was directly exposed to radiation. To test the role of GJICs and their major constituent connexin43 (Cx43), crosses between Ptch1{sup +/?} and Cx43{sup +/?} mice were similarly irradiated. These mouse groups were monitored for their lifetime, and skin basal cell carcinomas (BCCs) were counted and recorded. Early responses to DNA damage - Double Strand Breaks (DSBs) and apoptosis - were also evaluated in shielded and directly irradiated skin areas. Results: We report abscopal tumor induction in the shielded skin of Ptch1{sup +/?} mice after partial-body irradiation. Endpoints were induction of early nodular BCC-like tumors and macroscopic infiltrative BCCs. Abscopal tumorigenesis was significantly modulated by Cx43 status, namely, Cx43 reduction was associated with decreased levels of DNA damage and oncogenesis in out-of-field skin, suggesting a key role of GJIC in transmission of oncogenic radiation signals to unhit skin. Conclusions: Our results further characterize the nature of abscopal responses and the implications they have on pathologic processes in different tissues, including their possible underlying mechanistic bases.

  13. Capturing skin properties from dynamic mechanical analyses

    E-Print Network [OSTI]

    Sandford, Erika J. (Erika Jaye)

    2012-01-01

    Existing skin mechanical testing devices focus on measuring skin elasticity and are not tailored to assess the dynamic behavior of skin. The mathematical techniques used to analyze data collected using these devices are ...

  14. Three-Dimensional Model on Thermal Response of Skin Subject to Laser Heating

    E-Print Network [OSTI]

    Zhang, Jun

    thermal response. The time-dependent equation is discretized using the #12;nite di#11;erence methodThree-Dimensional Model on Thermal Response of Skin Subject to Laser Heating #3; Wensheng Shen y to investigate the transient thermal response of human skin subject to laser heating. The temperature

  15. Implantable apparatus for localized heating of tissue

    DOE Patents [OSTI]

    Doss, J.D.

    1985-05-20

    With the object of repetitively treating deep-seated, inoperable tumors by hyperthermia as well as locally heating other internal tissue masses repetitively, a receiving antenna, transmission line and electrode arrangement are implanted completely within the patient's body, with the receiving antenna just under the surface of the skin and with the electrode arrangement being located so as to most effectively heat the tissue to be treated. An external, transmitting antenna, driven by an external radio-frequency energy source, is closely coupled to the implanted receiving antenna so that the energy coupled across the air-skin interface provides electromagnetic energy suitable for heating the tissue in the vicinity of the implanted electrodes. The resulting increase in tissue temperature may be estimated by an indirect measurement of the decrease in tissue resistivity in the heat region. This change in resistivity appears as a change in the loading of the receiving antenna which can be measured by either determining the change in the phase relationship between the voltage and the current appearing on the transmitting antenna or by measuring the change in the magnitude of the impedance thereof. Optionally, multiple electrode arrays may be activated or inactivated by the application of magnetic fields to operate implanted magnetic reed swtiches. 5 figs.

  16. Implantable apparatus for localized heating of tissue

    DOE Patents [OSTI]

    Doss, James D. (Los Alamos, NM)

    1987-01-01

    With the object of repetitively treating deep-seated, inoperable tumors by hyperthermia as well as locally heating other internal tissue masses repetitively, a receiving antenna, transmission line, and electrode arrangment are implanted completely within the patient's body, with the receiving antenna just under the surface of the skin and with the electrode arrangement being located so as to most effectively heat the tissue to be treated. An external, transmitting antenna, driven by an external radio-frequency energy source, is closely coupled to the implanted receiving antenna so that the energy coupled across the air-skin interface provides electromagnetic energy suitable for heating the tissue in the vicinity of the implanted electrodes. The resulting increase in tissue temperature may be estimated by an indirect measurement of the decrease in tissue resistivity in the heated region. This change in resistivity appears as a change in the loading of the receiving antenna which can be measured by either determining the change in the phase relationship between the voltage and the current appearing on the transmitting antenna or by measuring the change in the magnitude of the impedance thereof. Optionally, multiple electrode arrays may be activated or inactivated by the application of magnetic fields to operate implanted magnetic reed switches.

  17. Profiling of Adrenocorticotropic Hormone and Arginine Vasopressin in Human Pituitary Gland and Tumor Thin Tissue Sections using Droplet-Based Liquid Microjunction Surface Sampling-HPLC-ESI-MS/MS

    SciTech Connect (OSTI)

    Kertesz, Vilmos; Van Berkel, Gary J; Calligaris, David; Feldman, Daniel R; Changelian, Armen; Laws, Edward R; Santagata, Sandro; Agar, Nathalie YR

    2015-01-01

    Described here are the results from the profiling of the proteins arginine vasopressin (AVP) and adrenocorticotropic hormone (ACTH) from normal human pituitary gland and pituitary adenoma tissue sections using a fully automated droplet-based liquid microjunction surface sampling-HPLC-ESI-MS/MS system for spatially resolved sampling, HPLC separation, and mass spectral detection. Excellent correlation was found between the protein distribution data obtained with this droplet-based liquid microjunction surface sampling-HPLC-ESI-MS/MS system and those data obtained with matrix assisted laser desorption ionization (MALDI) chemical imaging analyses of serial sections of the same tissue. The protein distributions correlated with the visible anatomic pattern of the pituitary gland. AVP was most abundant in the posterior pituitary gland region (neurohypophysis) and ATCH was dominant in the anterior pituitary gland region (adenohypophysis). The relative amounts of AVP and ACTH sampled from a series of ACTH secreting and non-secreting pituitary adenomas correlated with histopathological evaluation. ACTH was readily detected at significantly higher levels in regions of ACTH secreting adenomas and in normal anterior adenohypophysis compared to non-secreting adenoma and neurohypophysis. AVP was mostly detected in normal neurohypophysis as anticipated. This work demonstrates that a fully automated droplet-based liquid microjunction surface sampling system coupled to HPLC-ESI-MS/MS can be readily used for spatially resolved sampling, separation, detection, and semi-quantitation of physiologically-relevant peptide and protein hormones, such as AVP and ACTH, directly from human tissue. In addition, the relative simplicity, rapidity and specificity of the current methodology support the potential of this basic technology with further advancement for assisting surgical decision-making.

  18. Sensitive skins and somatic processing for affective and sociable robots based upon a somatic alphabet approach

    E-Print Network [OSTI]

    Stiehl, Walter Daniel, 1980-

    2005-01-01

    The sense of touch is one of the most important senses of the human body. This thesis describes the biologically inspired design of "sensitive skins" for two different robotic platforms: Leonardo, a high degree-of-freedom, ...

  19. Use of optical coherence tomography to monitor biological tissue freezing during cryosurgery

    E-Print Network [OSTI]

    Aguilar, Guillermo

    Use of optical coherence tomography to monitor biological tissue freezing during cryosurgery were acquired during freezing of water, IntralipidTM, and in vivo hamster skin. Sub- surface morphological changes were evident only during freezing of Intralipid and skin. A simple thermal model

  20. Mechanistic and quantitative studies of bystander response in 3D tissues for low-dose radiation risk estimations

    SciTech Connect (OSTI)

    Amundson, Sally A.

    2013-06-12

    We have used the MatTek 3-dimensional human skin model to study the gene expression response of a 3D model to low and high dose low LET radiation, and to study the radiation bystander effect as a function of distance from the site of irradiation with either alpha particles or low LET protons. We have found response pathways that appear to be specific for low dose exposures, that could not have been predicted from high dose studies. We also report the time and distance dependent expression of a large number of genes in bystander tissue. the bystander response in 3D tissues showed many similarities to that described previously in 2D cultured cells, but also showed some differences.

  1. Skinning Arbitrary Deformations Ladislav Kavan 1,2 Rachel McDonnell1 Simon Dobbyn1 Jiri Zara2 Carol O'Sullivan1

    E-Print Network [OSTI]

    Plotkin, Joshua B.

    , such as moving human or animal figures. In this paper, we demonstrate how to automatically construct skinning way of representing the animation of quasi- articulated objects, such as virtual characters, is known as skinning (or matrix palette skinning). It is based on the observation that an animation of a virtual

  2. A critical comparison of human face rendering techniques

    E-Print Network [OSTI]

    Arizpe, Arturo Andrew

    2006-01-01

    Human skin exhibits complex light reflectance properties that make it difficult to render realistically. In recent years, many techniques have been introduced to render skin, with varying degrees of complexity and realism. ...

  3. Biomaterials for Tissue Regeneration

    E-Print Network [OSTI]

    Alpay, S. Pamir

    Biomaterials for Tissue Regeneration Mei Wei, Associate Professor Materials Science and Engineering and pursuing PhD degree Strong interest in conducting research in "biomaterials for tissue regeneration

  4. Dynamic Skin Triangulation (extended abstract)

    E-Print Network [OSTI]

    Sullivan, John M.

    of physical simulation, where they act as bound- aries of spatial domains that grow and shrink with time stages [1]. Moving bound- aries also arise naturally in mold filling processes, both for metal and other and shrinking. The skin surface is the envelope of this family. Even though the family is infinite, the surface

  5. Gene Expression in Skin and Lymphoblastoid Cells: Refined Statistical Method Reveals

    E-Print Network [OSTI]

    Abecasis, Goncalo

    Rajan P. Nair,2 James T. Elder,2,6 and Goncalo R. Abecasis1,* Psoriasis, an immune to be associated with psoriasis than are randomly selected SNPs. To explore the tissue specificity of these e on psoriasis and other skin traits. Introduction Transcriptional regulation of gene expression is essential

  6. Structural colouration of mammalian skin: convergent evolution of coherently scattering dermal collagen arrays

    E-Print Network [OSTI]

    Prum, Richard O.; Torres, Rodolfo H.

    2004-05-01

    fast-green. For TEM, skin samples were post-fixed in 2?4% osmium tetroxide for 1.5?h. They were then stained with 2% aqueous uranyl acetate for 1?h. Tissue pieces were then dehydrated through an ethanol series and embedded in Eponate 12. They were...

  7. Turbine vane with high temperature capable skins

    DOE Patents [OSTI]

    Morrison, Jay A. (Oviedo, FL)

    2012-07-10

    A turbine vane assembly includes an airfoil extending between an inner shroud and an outer shroud. The airfoil can include a substructure having an outer peripheral surface. At least a portion of the outer peripheral surface is covered by an external skin. The external skin can be made of a high temperature capable material, such as oxide dispersion strengthened alloys, intermetallic alloys, ceramic matrix composites or refractory alloys. The external skin can be formed, and the airfoil can be subsequently bi-cast around or onto the skin. The skin and the substructure can be attached by a plurality of attachment members extending between the skin and the substructure. The skin can be spaced from the outer peripheral surface of the substructure such that a cavity is formed therebetween. Coolant can be supplied to the cavity. Skins can also be applied to the gas path faces of the inner and outer shrouds.

  8. Active skin for turbulent drag reduction

    E-Print Network [OSTI]

    Mani, Raghavendran

    2002-01-01

    capitalizes on recent advances in active turbulent drag reduction and active material based actuation to develop an active or "smart" skin for turbulent drag reduction in realistic flight conditions. The skin operation principle is based on computational...

  9. 2,6-Dithiopurine, a nucleophilic scavenger, protects against mutagenesis in mouse skin treated in vivo with 2-(chloroethyl) ethyl sulfide, a mustard gas analog

    SciTech Connect (OSTI)

    Boulware, Stephen [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States)] [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States); Fields, Tammy; McIvor, Elizabeth; Powell, K. Leslie; Abel, Erika L. [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States)] [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States); Vasquez, Karen M. [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States)] [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States); MacLeod, Michael C., E-mail: mcmacleod@mdanderson.org [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States)

    2012-09-01

    Sulfur mustard [bis(2-chloroethyl)sulfide, SM] is a well-known DNA-damaging agent that has been used in chemical warfare since World War I, and is a weapon that could potentially be used in a terrorist attack on a civilian population. Dermal exposure to high concentrations of SM produces severe, long-lasting burns. Topical exposure to high concentrations of 2-(chloroethyl) ethyl sulfide (CEES), a monofunctional analog of SM, also produces severe skin lesions in mice. Utilizing a genetically engineered mouse strain, Big Blue, that allows measurement of mutation frequencies in mouse tissues, we now show that topical treatment with much lower concentrations of CEES induces significant dose- and time-dependent increases in mutation frequency in mouse skin; the mutagenic exposures produce minimal toxicity as determined by standard histopathology and immunohistochemical analysis for cytokeratin 6 and the DNA-damage induced phosphorylation of histone H2AX (?-H2AX). We attempted to develop a therapeutic that would inhibit the CEES-induced increase in mutation frequency in the skin. We observe that multi-dose, topical treatment with 2,6-dithiopurine (DTP), a known chemical scavenger of CEES, beginning 1 h post-exposure to CEES, completely abolishes the CEES-induced increase in mutation frequency. These findings suggest the possibility that DTP, previously shown to be non-toxic in mice, may be useful as a therapeutic agent in accidental or malicious human exposures to SM. -- Highlights: ? 200 mM 2-(chloroethyl) ethyl sulfide (CEES) induces mutations in mouse skin. ? This dose of CEES is not overtly toxic, as assayed by histopathology. ? 2,6-Dithiopurine (DTP), applied after CEES-treatment, abolishes CEES-mutagenesis. ? This supports the idea that sulfur mustards exhibit long biological half-lives.

  10. Apparatus for testing skin samples or the like

    DOE Patents [OSTI]

    Holland, J.M.

    1982-08-31

    An apparatus for testing the permeability of living skin samples has a flat base with a plurality of sample-holding cavities formed in its upper surface, the samples being placed in counterbores in the cavities with the epidermis uppermost. O-rings of Teflon washers are respectively placed on the samples and a flat cover is connected to the base to press the rings against the upper surfaces of the samples. Media to maintain tissue viability and recovery of metabolites is introduced into the lower portion of the sample-holding cavities through passages in the base. Test materials are introduced through holes in the cover plate after assembly of the chamber.

  11. Near-infrared spectroscopic tissue imaging for medical applications

    DOE Patents [OSTI]

    Demos; Stavros (Livermore, CA), Staggs; Michael C. (Tracy, CA)

    2006-03-21

    Near infrared imaging using elastic light scattering and tissue autofluorescence are explored for medical applications. The approach involves imaging using cross-polarized elastic light scattering and tissue autofluorescence in the Near Infra-Red (NIR) coupled with image processing and inter-image operations to differentiate human tissue components.

  12. Near-infrared spectroscopic tissue imaging for medical applications

    DOE Patents [OSTI]

    Demos, Stavros (Livermore, CA); Staggs, Michael C. (Tracy, CA)

    2006-12-12

    Near infrared imaging using elastic light scattering and tissue autofluorescence are explored for medical applications. The approach involves imaging using cross-polarized elastic light scattering and tissue autofluorescence in the Near Infra-Red (NIR) coupled with image processing and inter-image operations to differentiate human tissue components.

  13. Investigation of in-vivo skin autofluorescence lifetimes under long-term cw optical excitation

    SciTech Connect (OSTI)

    Lihachev, A; Ferulova, I; Vasiljeva, K; Spigulis, J

    2014-08-31

    The main results obtained during the last five years in the field of laser-excited in-vivo human skin photobleaching effects are presented. The main achievements and results obtained, as well as methods and experimental devices are briefly described. In addition, the impact of long-term 405-nm cw low-power laser excitation on the skin autofluorescence lifetime is experimentally investigated. (laser biophotonics)

  14. Time-Domain Polarization-Sensitive Optical Coherence Tomography in Soft Biological Tissue

    E-Print Network [OSTI]

    Todorovic, Milos

    2009-05-15

    measurements of a section of porcine tendon and the septum of a rat heart. The application of the system for burn imaging and healing monitoring was demonstrated on porcine skin because of its similarity to the human skin. The results showed a clear...

  15. Chronic cellular responses of rat skin to 13 Mev proton irradiation

    E-Print Network [OSTI]

    Hinkle, Donald King

    1966-01-01

    irradiated in a total of six rad groups as follows: Number in Grou dD 10 6 9 6 7 8 ZOO 400 700 1300 2000 2500 All sections of skin and tumor tissues were submitted to the Anatomic Pathology Section, USAF School of Aerospace Medicine...CHRONIC CELLULAR RESPONSES OF RAT SKIN TO 13 MEV PROTON IRRADIATION A Thesis by DONALD KING HINKLE, D. V. M. Submitted to the Graduate College of the Texas AErM University in partial fulfillment of the requirements for the degree of MASTER...

  16. Stationary turbine component with laminated skin

    DOE Patents [OSTI]

    James, Allister W. (Orlando, FL)

    2012-08-14

    A stationary turbine engine component, such as a turbine vane, includes a internal spar and an external skin. The internal spar is made of a plurality of spar laminates, and the external skin is made of a plurality of skin laminates. The plurality of skin laminates interlockingly engage the plurality of spar laminates such that the external skin is located and held in place. This arrangement allows alternative high temperature materials to be used on turbine engine components in areas where their properties are needed without having to make the entire component out of such material. Thus, the manufacturing difficulties associated with making an entire component of such a material and the attendant high costs are avoided. The skin laminates can be made of advanced generation single crystal superalloys, intermetallics and refractory alloys.

  17. Skewed distribution of natural killer cells in psoriasis skin lesions

    E-Print Network [OSTI]

    2013-01-01

    of natural killer cells in psoriasis skin lesions Mariana D.and unaf- fected skin of psoriasis patients and normal skinComparisons are made between psoriasis groups (lesional and

  18. Design and potential clinical impact of a noninvasive thermal diffusion sensor to monitor human peripheral microvascular perfusion in real-time

    E-Print Network [OSTI]

    Li, Vivian V. (Vivian Victoria)

    2006-01-01

    Perfusion in peripheral tissues is fundamental to the characterization of both local and global cardiovascular health. However, despite the inherent accessibility of tissues such as skin to microvascular measurements, there ...

  19. Bio-inspired nanocomposite assemblies as smart skin components...

    Office of Scientific and Technical Information (OSTI)

    Bio-inspired nanocomposite assemblies as smart skin components. Citation Details In-Document Search Title: Bio-inspired nanocomposite assemblies as smart skin components. There is...

  20. Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Contracts. Final report

    SciTech Connect (OSTI)

    Cornforth, Michael N.

    2013-05-03

    Combining contemporary cytogenetic methods with DNA CGH microarray technology and chromosome flow-sorting increases substantially the ability to resolve exchange breakpoints associated with interstitial deletions and translocations, allowing the consequences of radiation damage to be directly measured at low doses, while also providing valuable insights into molecular mechanisms of misrepair processes that, in turn, identify appropriate biophysical models of risk at low doses. The aims of this work apply to cells recovered from 3D tissue constructs of human skin and, for the purpose of comparison, the same cells irradiated in traditional 2D cultures. These aims are: to analyze by multi-flour fluorescence in situ hybridization (mFISH) the chromosomes in clonal descendents of individual human fibroblasts that were previously irradiated; to examine irradiated clones from Aim 1 for submicroscopic deletions by subjecting their DNA to comparative genomic hybridization (CGH) microarray analysis; and to flow-sort aberrant chromosomes from clones containing stable radiation-induced translocations and map the breakpoints to within an average resolution of 100 kb using the technique of 'array painting'.

  1. Skin thickness effects on in vivo LXRF

    SciTech Connect (OSTI)

    Preiss, I.L.; Washington, W. II

    1995-12-31

    The analysis of lead concentration in bone utilizing LXRF can be adversely effected by overlying issue. A quantitative measure of the attenuation of the 10.5 keV Pb L a x-ray signal by skin and skin equivalent plastic has been conducted. Concentration ranges in plaster of Paris and goat bone from 7 to 90 ppm with attenuators of Lucite{reg_sign} and pig skin were examined. It is concluded that no quantitative or semi quantitative analysis can be achieved if overlying sue thickness exceeds 3 mm for Ph concentrations of less than 30 porn Ph in bone.

  2. Nonlinear stochastic system identification techniques for biological tissues/

    E-Print Network [OSTI]

    Chen, Yi, S.M. Massachusetts Institute of Technology

    2010-01-01

    This research develops a device capable of measuring the nonlinear dynamic mechanical properties of human tissue in vivo. The enabling technology is the use of nonlinear stochastic system identification techniques in ...

  3. Differentiation of normal and cancerous lung tissues by multiphoton imaging

    E-Print Network [OSTI]

    Wang, Chun-Chin

    We utilize multiphoton microscopy for the label-free diagnosis of noncancerous, lung adenocarcinoma (LAC), and lung squamous cell carcinoma (SCC) tissues from humans. Our results show that the combination of second-harmonic ...

  4. 'Epidermal Electronics' Paste Peelable Circuitry On Your Skin, Just Like A Temporary Tattoo | Popular Science http://www.popsci.com/science/article/2011-08/epidermal-electronics-paste-peelable-circuitry-your-skin-just-temporary-tattoo[8/14/2011 5:57:38 AM

    E-Print Network [OSTI]

    Rogers, John A.

    - Powered Upgrade New Electric Skin Could Bring the Human Touch to Robots, Artificial Limbs Optical Sensors adhere to the skin not with glue or static electricity, but close-contact atomic forces called van der capacitors and wireless antennas, according to UI. The devices can draw power from induction or even from

  5. The skin's role in human thermoregulation and comfort

    E-Print Network [OSTI]

    Arens, Edward A; Zhang, H.

    2006-01-01

    with sensory organs in the hypothalamus (within the brain),within the anterior hypothalamus sense the core temperatureit. The anterior hypothalamuss warm sensors outnumber its

  6. Athletic equipment microbiota are shaped by interactions with human skin

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnical Report:Speeding accessby a contractor ofvarDOE PAGES11 PPPL- 4811

  7. Athletic equipment microbiota are shaped by interactions with human skin

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnical Report:Speeding accessby a contractor ofvarDOE PAGES11 PPPL- 4811(Journal

  8. Athletic equipment microbiota are shaped by interactions with human skin

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnical Report:Speeding accessby a contractor ofvarDOE PAGES11 PPPL-

  9. Athletic equipment microbiota are shaped by interactions with human skin

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing Bacteria (Technical Report) | SciTech Connect Assessing theopacityArticle)Wood et

  10. Short and ultrashort laser pulse induced bubbles on transparent and scattering tissue models.

    E-Print Network [OSTI]

    Aguilar, Guillermo

    models that mimic biological tissue were irradiated with a Q-switched, 532 nm, 5 nanosecond, Nd cases where the treatment is not totally successful; as an example, Port Wine Stain (PWS) birthmarks are an abnormal layer of blood vessels 10 to 100m in diameter localized 100 to 500 m below the skin surface

  11. INSTITUTE OF PHYSICS PUBLISHING PHYSICS IN MEDICINE AND BIOLOGY Phys. Med. Biol. 48 (2003) N15N24 PII: S0031-9155(03)52641-0

    E-Print Network [OSTI]

    Choi, Bernard

    2003-01-01

    agents and ex vivo human skin: implications for depth profiling of human skin John A Viator1 , Bernard measurements have been used to profile or image biological tissue, including human skin. Usually, analysis be reasonable for soft tissue, but introduction of exogenous agents into skin or the measurement of tissue

  12. Engineering Cell and Tissue Mechanical Microenvironments for Regenerative Medicine

    E-Print Network [OSTI]

    Tsou, Danielle An-Chi

    2012-01-01

    in arterial tissue engineering. , Journal of neural tissue engineering. , Biomaterials, vol. for cardiovascular tissue engineering. , Tissue

  13. Tissue-like phantoms

    DOE Patents [OSTI]

    Frangioni, John V. (Wayland, MA); De Grand, Alec M. (Boston, MA)

    2007-10-30

    The invention is based, in part, on the discovery that by combining certain components one can generate a tissue-like phantom that mimics any desired tissue, is simple and inexpensive to prepare, and is stable over many weeks or months. In addition, new multi-modal imaging objects (e.g., beads) can be inserted into the phantoms to mimic tissue pathologies, such as cancer, or merely to serve as calibration standards. These objects can be imaged using one, two, or more (e.g., four) different imaging modalities (e.g., x-ray computed tomography (CT), positron emission tomography (PET), single photon emission computed tomography (SPECT), and near-infrared (NIR) fluorescence) simultaneously.

  14. Dynamic Skin Triangulation Ho-Lun Cheng

    E-Print Network [OSTI]

    Edelsbrunner, Herbert

    that grow and shrink with time. An example is the boundary between the solid and the liquid portions the nu- cleation, growth and coarsening stages [1]. Moving bound- aries also arise naturally in mold the finite collection by convex combina- tion and shrinking. The skin surface is the envelope of this family

  15. Dynamic Skin Triangulation Ho-Lun Cheng

    E-Print Network [OSTI]

    Sullivan, John M.

    of physical simulation, where they act as bound- aries of spatial domains that grow and shrink with time stages [1]. Moving bound- aries also arise naturally in mold filling processes, both for metal and other and shrinking. The skin surface is the envelope of this family. Even though the family is infinite, the surface

  16. Space radiation-induced bystander signaling in 2D and 3D skin tissue models

    E-Print Network [OSTI]

    Lumpkins, Sarah B

    2012-01-01

    Space radiation poses a significant hazard to astronauts on long-duration missions, and the low fluences of charged particles characteristic of this field suggest that bystander effects, the phenomenon in which a greater ...

  17. Chemical Agent Induced Reduction of Skin Light Scattering

    E-Print Network [OSTI]

    Hirshburg, Jason M.

    2011-02-22

    Skin turbidity limits light based medical applications while increasing the risk of epidermal thermal injury. Collagen fibers are responsible for the majority of light scattering within skin. Chemicals, known as clearing ...

  18. Vaccine delivery with microneedle skin patches in nonhuman primates

    E-Print Network [OSTI]

    Li, Adrienne V

    Transcutaneous drug delivery from planar skin patches is effective for small-molecule drugs and skin-permeable vaccine adjuvants. However, to achieve efficient delivery of vaccines and other macromolecular therapeutics ...

  19. Dietary chromium and nickel enhance UV-carcinogenesis in skin...

    Office of Scientific and Technical Information (OSTI)

    chromium and nickel enhance UV-carcinogenesis in skin of hairless mice The skin cancer enhancing effect of chromium (in male mice) and nickel in UVR-irradiated female Skh1...

  20. Fibre Diffraction Analysis of Skin Offers a Very Early and Extremely Accurate Diagnostic Test for Prostate Cancer

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    James, Veronica J.; OMalley Ford, Judith M.

    2014-01-01

    Double blind analysis of a batch of thirty skin tissue samples from potential prostate cancer sufferers correctly identified all control patients, patients with high and low grade prostate cancers, the presence of benign prostate hyperplasia (BPH), perineural invasions, and the one lymphatic invasion. Identification was by analysis of fibre diffraction patterns interpreted using a schema developed from observations in nine previous studies. The method, schema, and specific experiment results are reported in this paper, with some implications then drawn.

  1. Fluorometric Tissue-based Biosensors

    SciTech Connect (OSTI)

    Greenbaum, Elias; Woodward, Charlene; Rodriguez, Jr., Miguel

    2000-11-30

    Experimental program to develop tissue-based biosensors for detection of airborne chemical warfare agents and water quality monitoring.

  2. Structure, function and diversity of the healthy human microbiome

    E-Print Network [OSTI]

    Alm, Eric J.

    Studies of the human microbiome have revealed that even healthy individuals differ remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of this diversity remains unexplained, although diet, ...

  3. Biomechanics of the human chorioamnion

    E-Print Network [OSTI]

    Prvost, Thibault Philippe

    2006-01-01

    The human fetal membrane, namely the chorioamnion, is the structural soft tissue retaining the amniotic fluid and the fetus during pregnancy. Its biomechanical integrity is crucial for maintaining a healthy gestation and ...

  4. Turbine blade having a constant thickness airfoil skin

    DOE Patents [OSTI]

    Marra, John J

    2012-10-23

    A turbine blade is provided for a gas turbine comprising: a support structure comprising a base defining a root of the blade and a framework extending radially outwardly from the base, and an outer skin coupled to the support structure framework. The skin has a generally constant thickness along substantially the entire radial extent thereof. The framework and the skin define an airfoil of the blade.

  5. Reflective Terahertz Imaging for early diagnosis of skin burn severity

    E-Print Network [OSTI]

    TEWARI, PRIYAMVADA

    2013-01-01

    regions of skin, burns, psoriasis, basal cell carcinomas andinto diseased states like psoriasis, eczema, basal celland cancer, conditions like psoriasis, eczema, dermatitis,

  6. The Direction of Optimal Skin Incisions Derived From Striae Distensae.

    E-Print Network [OSTI]

    Lemperle, Gottfried

    2015-01-01

    of Optimal Skin Incisions Derived from Striae Distensaestriae lines. These are derived from the striae compositecal incisions and scars derived from the Internet. Incision

  7. TOWARDS AUTOMATED SKIN LESION DIAGNOSIS Paul Wighton

    E-Print Network [OSTI]

    Atkins, M. Stella

    for minimal risk human research, by the Office of Research Ethics. A copy of the approval letter has been be improved. Typically, the ASLD pipeline consists of

  8. Molecular substrate design for the selective adhesion, proliferation and differentiation of marrow connective tissue progenitors

    E-Print Network [OSTI]

    Au, Ada

    2005-01-01

    A multi-faceted approach was applied to the molecular design of substrates for the selective adhesion, proliferation and differentiation of connective tissue progenitors (CTPs) from human bone marrow aspirates. The basic ...

  9. Integration of real time oxygen measurements with a 3D perfused tissue culture system

    E-Print Network [OSTI]

    Inman, Samuel Walker

    2011-01-01

    In vitro models that capture the complexity of human tissue and organ behaviors in a scalable and easy-to- use format are of increasing interest for both technological applications in drug development and in basic biology ...

  10. Cellular resolution ex vivo imaging of gastrointestinal tissues with coherence microscopy

    E-Print Network [OSTI]

    Fujimoto, James G.

    Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex ...

  11. Can OCT be sensitive to nanoscale structural alterations in biological tissue?

    E-Print Network [OSTI]

    Taflove, Allen

    is validated by 1) numerical simulations; 2) tissue phantom studies; and 3) ex vivo colon tissue measurements is demonstrated with ex vivo rat buccal and human colon samples. 2013 Optical Society of America OCIS codes: (170 resolution," Science 313(5793), 16421645 (2006). #181995 - $15.00 USD Received 3 Jan 2013; revised 7 Mar

  12. Detecting pornographic images by localizing skin Sotiris Karavarsamisa

    E-Print Network [OSTI]

    Blekas, Konstantinos

    specialized sub- classes, namely "bikini" / "porn" and "skin" / "non-skin", respectively. The extracted pornographic image classifiers. Index Terms convex hull calculation, multi-class classification, porn detection unprotected Web services in order to circulate or exchange child pornography and general pornographic content

  13. INVESTIGATION The Lsktm1 Locus Modulates Lung and Skin

    E-Print Network [OSTI]

    Broman, Karl W.

    INVESTIGATION The Lsktm1 Locus Modulates Lung and Skin Tumorigenesis in the Mouse Antonella Galvan to both skin and lung tumorigenesis over the susceptibility of the SWR/J strain. In an effort to map tumor.93) and lung (LOD score = 8.74) tumorigenesis. Two genes, Igfbp5 and Igfbp2, residing in this locus

  14. Neutron skin of 208 Pb in consistency with

    E-Print Network [OSTI]

    Neutron skin of 208 Pb in consistency with neutron star observations K. Miyazaki E-mail: miyazakiro as varying the neutron radius of 208Pb. The neutron skin thickness Sn is determined in the comparison with the astronomical observations of massive neutron stars (NSs), the standard scenario of NS cooling

  15. Skin cancer detection by oblique-incidence diffuse reflectance spectroscopy

    E-Print Network [OSTI]

    Smith, Elizabeth Brooks

    2009-05-15

    Skin cancer is the most common form of cancer and it is on the rise. If skin cancer is diagnosed early enough, the survival rate is close to 90%. Oblique-incidence diffuse reflectance (OIR) spectroscopy offers a technology that may be used...

  16. Mechanisms of mesothelial tissue lubrication

    E-Print Network [OSTI]

    Lin, Judy Li-Wen

    2006-01-01

    In the pleural space, sliding between the lung and chest wall induces shear stress that could damage the delicate mesothelial cells covering the tissue surfaces. Normally, the pleural space, which is filled with fluid, is ...

  17. Mechanistic investigation of skin barrier perturbation induced by surfactants in the presence of humectants

    E-Print Network [OSTI]

    Ghosh, Saswata

    2007-01-01

    The stratum corneum (SC) of the skin functions as a barrier between the body and the environment. Surfactants such as Sodium Dodecyl Sulfate (SDS) are used in skin cleansers and in skin-care formulations because of their ...

  18. Compact biomedical pulsed signal generator for bone tissue stimulation

    DOE Patents [OSTI]

    Kronberg, J.W.

    1993-06-08

    An apparatus for stimulating bone tissue for stimulating bone growth or treating osteoporosis by applying directly to the skin of the patient an alternating current electrical signal comprising wave forms known to simulate the piezoelectric constituents in bone. The apparatus may, by moving a switch, stimulate bone growth or treat osteoporosis, as desired. Based on low-power CMOS technology and enclosed in a moisture-resistant case shaped to fit comfortably, two astable multivibrators produce the desired waveforms. The amplitude, pulse width and pulse frequency, and the subpulse width and subpulse frequency of the waveforms are adjustable. The apparatus, preferably powered by a standard 9-volt battery, includes signal amplitude sensors and warning signals indicate an output is being produced and the battery needs to be replaced.

  19. Compact biomedical pulsed signal generator for bone tissue stimulation

    DOE Patents [OSTI]

    Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

    1993-01-01

    An apparatus for stimulating bone tissue for stimulating bone growth or treating osteoporosis by applying directly to the skin of the patient an alternating current electrical signal comprising wave forms known to simulate the piezoelectric constituents in bone. The apparatus may, by moving a switch, stimulate bone growth or treat osteoporosis, as desired. Based on low-power CMOS technology and enclosed in a moisture-resistant case shaped to fit comfortably, two astable multivibrators produce the desired waveforms. The amplitude, pulse width and pulse frequency, and the subpulse width and subpulse frequency of the waveforms are adjustable. The apparatus, preferably powered by a standard 9-volt battery, includes signal amplitude sensors and warning signals indicate an output is being produced and the battery needs to be replaced.

  20. Analysis of Symmetry in the Anterior Human Dentition and its Application in the Evaluation and Correction of Postural Distortion in the Photographic Recording of Human Bite Marks

    E-Print Network [OSTI]

    Aws, Ghassan

    Postural distortion of human bite marks on skin occurs when photographing a bite mark in a body position other than the position of the body at the time of biting. Postural distortion in the bite mark may introduce ...

  1. Skin-sparing Helical Tomotherapy vs 3D-conformal Radiotherapy for Adjuvant Breast Radiotherapy: In Vivo Skin Dosimetry Study

    SciTech Connect (OSTI)

    Capelle, Lisa; Warkentin, Heather; MacKenzie, Marc; Joseph, Kurian; Gabos, Zsolt; Pervez, Nadeem; Tankel, Keith; Chafe, Susan; Amanie, John; Ghosh, Sunita; Parliament, Matthew; Abdulkarim, Bassam

    2012-08-01

    Purpose: We investigated whether treatment-planning system (TPS)-calculated dose accurately reflects skin dose received for patients receiving adjuvant breast radiotherapy (RT) with standard three-dimensional conformal RT (3D-CRT) or skin-sparing helical tomotherapy (HT). Methods and Materials: Fifty patients enrolled in a randomized controlled trial investigating acute skin toxicity from adjuvant breast RT with 3D-CRT compared to skin-sparing HT, where a 5-mm strip of ipsilateral breast skin was spared. Thermoluminescent dosimetry or optically stimulated luminescence measurements were made in multiple locations and were compared to TPS-calculated doses. Skin dosimetric parameters and acute skin toxicity were recorded in these patients. Results: With HT there was a significant correlation between calculated and measured dose in the medial and lateral ipsilateral breast (r = 0.67, P<.001; r = 0.44, P=.03, respectively) and the medial and central contralateral breast (r = 0.73, P<.001; r = 0.88, P<.001, respectively). With 3D-CRT there was a significant correlation in the medial and lateral ipsilateral breast (r = 0.45, P=.03; r = 0.68, P<.001, respectively); the medial and central contralateral breast (r = 0.62, P=.001; r = 0.86, P<.001, respectively); and the mid neck (r = 0.42, P=.04, respectively). On average, HT-calculated dose overestimated the measured dose by 14%; 3D-CRT underestimated the dose by 0.4%. There was a borderline association between highest measured skin dose and moist desquamation (P=.05). Skin-sparing HT had greater skin homogeneity (homogeneity index of 1.39 vs 1.65, respectively; P=.005) than 3D-CRT plans. HT plans had a lower skin{sub V50} (1.4% vs 5.9%, respectively; P=.001) but higher skin{sub V40} and skin{sub V30} (71.7% vs 64.0%, P=.02; and 99.0% vs 93.8%, P=.001, respectively) than 3D-CRT plans. Conclusion: The 3D-CRT TPS more accurately reflected skin dose than the HT TPS, which tended to overestimate dose received by 14% in patients receiving adjuvant breast RT.

  2. Is the duration of skin disease visits decreasing in the united states?

    E-Print Network [OSTI]

    Davis, Scott A; Feldman, Steven R; Fleischer Jr., Alan B

    2015-01-01

    2). Visits for impetigo, psoriasis, and unspecified disorderneoplasm of skin (216.9) Psoriasis (696.1) unspecifiedneoplasm of skin (216.9) Psoriasis (696.1) Unspecified

  3. The Kauai Skin Cancer Study--1983 to 1992

    SciTech Connect (OSTI)

    Reizner, G.T. )

    1993-05-01

    The Kauai Skin Cancer Study began as a modest effort in 1983 to look at this island's skin cancer incidence. David Elpern MD, Kauai's only dermatologist at the time, was interested in the large number of these tumors in his practice. He first enlisted his office staff to help keep track of the numbers and type of these skin cancers. Along with this information, the basic demographic data on each patient was collected. These records became the first entries into what has become a decade-long project.

  4. Carmichael's Concise Review Microscopy is Only Skin Deep

    E-Print Network [OSTI]

    Heller, Eric

    Carmichael's Concise Review Microscopy is Only Skin Deep Stephen W. Carmichael Mayo Clinic. Coming Events 2011 EMAS 2011 May 1519, 2011 Angers, France www.emas-web.net IUMAS-V May 2227, 2011

  5. Mpemba paradox: Hydrogen bond memory and water-skin supersolidity

    E-Print Network [OSTI]

    Chang Q Sun

    2015-01-05

    Numerical reproduction of measurements, experimental evidence for skin super-solidity and hydrogen-bond memory clarified that Mpemba paradox integrates the heat emission-conduction-dissipation dynamics in the source-path-drain cycle system.

  6. Involvement of TGF-beta in skin photoaging

    E-Print Network [OSTI]

    Choi, Won Seon, 1975-

    2005-01-01

    The goal of this thesis study was to understand the role of TGF-[beta] in skin photoaging, especially in solar elastosis. Solar elastosis, the accumulation of elastotic material in the dermal extracelluar matrix, is a major ...

  7. ORIGINAL ARTICLE Bronchiectasis in Persons With Skin Lesions

    E-Print Network [OSTI]

    California at Berkeley, University of

    years of chronic cough underwent high-resolution computed tomography (CT); these scans were read reported chronic cough, CT evidence of bronchiectasis was found in 18 (67%) participants with skin lesions

  8. Tissue architecture: the ultimate regulator of breast epithelial function

    SciTech Connect (OSTI)

    Bissell, Mina J; Rizki, Aylin; Mian, Saira

    2003-10-20

    A problem in developmental biology that continues to take center stage is how higher organisms generate diverse tissues and organs given the same cellular genotype. In cell and tumor biology, the key question is not the production of form, but its preservation: how do tissues and organs maintain homeostasis, and how do cells within tissues lose or overcome these controls in cancer? Undoubtedly, mechanisms that maintain tissue specificity should share features with those employed to drive formation of the tissues. However, they are unlikely to be identical. At a simplistic level, developmental pathways may be thought of as a series of extremely rapid short-term events. Each new step depends on what came before, and the outcome is the organism itself at birth. All organs, with a few notable exceptions, such as the mammary gland and the brain, 'arrive' together and are complete when the organism is born. In mice and humans, these events occur in a mere 21 days and 9 months respectively. The stability of the differentiated state and the homeostasis of the organism, on the other hand, will last 40-110 times longer. How does the organism achieve this feat? How are tissues maintained? These questions also relate fundamentally to how tissues become malignant and, although not discussed here, to aging. While there is much literature on differentiation - loosely defined as the gain of a single or a series of functions - we know much less about the forces and the pathways that maintain organ morphology and function as a unit. This may be partly because it is difficult to study a tissue as a unit in vivo and there are few techniques that allow maintenance of organs in vitro long enough and in such a way as to make cell and molecular biology experiments possible. Techniques for culturing cells in three-dimensional gels (3D) as a surrogate for tissues, however, have been steadily improving and the method is now used by several laboratories. In this commentary we discuss the following: first, how our laboratory came to develop a model of the mammary gland acinus; second, what this model has told us about mechanisms that govern tissue specificity and malignancy; and third, possible directions for future studies. We summarize the evidence for the central role of ECM signaling in the maintenance of mammary function in culture and (more briefly) its role in tumorigenesis. This is followed by a discussion of the role that tissue architecture and tissue polarity (as opposed to cell polarity) may play in these processes. In an elegantly written and reasoned essay, Kirschner et al. coined the new science of developmental biology 'molecular vitalism'. They framed new concepts for self-organization as well as schemes for information flow in biological organization. Rao et al. reviewed and elaborated on differential-equation-based models of biochemical reaction networks and intracellular noise, with emphasis on bacteria and phage. Similarly, Hartwell et al. discussed the synergy between experiment and theory in elucidating 'modules' - collections of interacting molecules - and in unraveling how these modules collaborate to perform cellular functions such as signal transduction. We believe that many of these ideas will also be applicable to the maintenance of tissue specificity. As much as we agree with Kirschner et al. regarding the limitations of the machine analogy to biological systems, we conclude with thoughts on how we may proceed to model the complex tissue networks that govern breast tissue architecture. We suggest that our understanding of the structure and function of breast tissue would benefit from examining recent techniques for modeling large complex networks such as the World Wide Web and the Internet backbone among others.

  9. 3D Tissue Scaffolds BIOMATERIALS

    E-Print Network [OSTI]

    3D Tissue Scaffolds BIOMATERIALS Our goal is to develop measurement tools and reference materials006497-01) in collaboration with the New Jersey Center for Biomaterials (RESBIO P41 EB 001046). We have-material interactions have focused on planar (2D) surfaces or films. However, biomaterials are commonly used in 3D

  10. Apparatus and method to control atmospheric water vapor composition and concentration during dynamic cooling of biological tissues in conjunction with laser irradiations

    DOE Patents [OSTI]

    Nelson, J. Stuart (Laguna Niguel, CA); Anvari, Bahman (Houston, TX); Tanenbaum, B. Samuel (Irvine, CA); Milner, Thomas E. (Austin, TX)

    1999-01-01

    Cryogen spray cooling of skin surface with millisecond cryogen spurts is an effective method for establishing a controlled temperature distribution in tissue and protecting the epidermis from nonspecific thermal injury during laser mediated dermatological procedures. Control of humidity level, spraying distance and cryogen boiling point is material to the resulting surface temperature. Decreasing the ambient humidity level results in less ice formation on the skin surface without altering the surface temperature during the cryogen spurt. For a particular delivery nozzle, increasing the spraying distance to 85 millimeters lowers the surface temperature. The methodology comprises establishing a controlled humidity level in the theater of operation of the irradiation site of the biological tissues before and/or during the cryogenic spray cooling of the biological tissue. At cold temperatures calibration was achieved by mounting a thermistor on a thermoelectric cooler. The thermal electric cooler was cooled from from 20.degree. C. to about -20.degree. C. while measuring its infrared emission.

  11. Physical Properties of Blue Shark Useful in Designing a Skinning Machine

    E-Print Network [OSTI]

    Physical Properties of Blue Shark Useful in Designing a Skinning Machine D. E. BROWN, R. PAUL SINGH for a machine to skin blue shark 2 A wider market for blue shark products is being sought. For example, if removed in one piece the skin is of value for making leather. The machine proposed to skin blue shark

  12. Wireless power transmission for medical applications

    E-Print Network [OSTI]

    Payne, Joshua E.

    We studied the wireless power transmission capabilities of microwave through human skin-tissue. Microwave transmission through simulated human skins was tested with rectenna array as a power receiver located under the ...

  13. Intraluminal tissue welding for anastomosis

    DOE Patents [OSTI]

    Glinsky, M.; London, R.; Zimmerman, G.; Jacques, S.

    1998-10-27

    A method and device are provided for performing intraluminal tissue welding for anastomosis of a hollow organ. A retractable catheter assembly is delivered through the hollow organ and consists of a catheter connected to an optical fiber, an inflatable balloon, and a biocompatible patch mounted on the balloon. The disconnected ends of the hollow organ are brought together on the catheter assembly, and upon inflation of the balloon, the free ends are held together on the balloon to form a continuous channel while the patch is deployed against the inner wall of the hollow organ. The ends are joined or ``welded`` using laser radiation transmitted through the optical fiber to the patch. A thin layer of a light-absorbing dye on the patch can provide a target for welding. The patch may also contain a bonding agent to strengthen the bond. The laser radiation delivered has a pulse profile to minimize tissue damage. 8 figs.

  14. Intraluminal tissue welding for anastomosis

    DOE Patents [OSTI]

    Glinsky, Michael (Livermore, CA); London, Richard (Orinda, CA); Zimmerman, George (Lafayette, CA); Jacques, Steven (Portland, OR)

    1998-10-27

    A method and device are provided for performing intraluminal tissue welding for anastomosis of a hollow organ. A retractable catheter assembly is delivered through the hollow organ and consists of a catheter connected to an optical fiber, an inflatable balloon, and a biocompatible patch mounted on the balloon. The disconnected ends of the hollow organ are brought together on the catheter assembly, and upon inflation of the balloon, the free ends are held together on the balloon to form a continuous channel while the patch is deployed against the inner wall of the hollow organ. The ends are joined or "welded" using laser radiation transmitted through the optical fiber to the patch. A thin layer of a light-absorbing dye on the patch can provide a target for welding. The patch may also contain a bonding agent to strengthen the bond. The laser radiation delivered has a pulse profile to minimize tissue damage.

  15. Mechanical formalism for tissue dynamics

    E-Print Network [OSTI]

    Sham Tlili; Cyprien Gay; Francois Graner; Philippe Marcq; Franois Molino; Pierre Saramito

    2014-12-23

    The understanding of morphogenesis in living organisms has been renewed by tremendous progress in experimental techniques that provide access to cell-scale, quantitative information both on the shapes of cells within tissues and on the genes being expressed. This information suggests that our understanding of the respective contributions of gene expression and mechanics, and of their crucial entanglement, will soon leap forward. Biomechanics increasingly benefits from models, which assist the design and interpretation of experiments, point out the main ingredients and assumptions, and ultimately lead to predictions. The newly accessible local information thus calls for a reflection on how to select suitable classes of mechanical models. We review both mechanical ingredients suggested by the current knowledge of tissue behaviour, and modelling methods that can help generate a rheological diagram or a constitutive equation. We distinguish cell scale ("intra-cell") and tissue scale ("inter-cell") contributions. We recall the mathematical framework developped for continuum materials and explain how to transform a constitutive equation into a set of partial differential equations amenable to numerical resolution. We show that when plastic behaviour is relevant, the dissipation function formalism appears appropriate to generate constitutive equations; its variational nature facilitates numerical implementation, and we discuss adaptations needed in the case of large deformations. The present article gathers theoretical methods that can readily enhance the significance of the data to be extracted from recent or future high throughput biomechanical experiments.

  16. Gelatin based on Power-gel.TM. as solders for Cr.sup.4+laser tissue welding and sealing of lung air leak and fistulas in organs

    DOE Patents [OSTI]

    Alfano, Robert R.; Tang, Jing; Evans, Jonathan M.; Ho, Peng Pei

    2006-04-25

    Laser tissue welding can be achieved using tunable Cr.sup.4+ lasers, semiconductor lasers and fiber lasers, where the weld strength follows the absorption spectrum of water. The use of gelatin and esterified gelatin as solders in conjunction with laser inducted tissue welding impart much stronger tensile and torque strengths than albumin solders. Selected NIR wavelength from the above lasers can improve welding and avoid thermal injury to tissue when used alone or with gelatin and esterified gelatin solders. These discoveries can be used to enhance laser tissue welding of tissues such as skin, mucous, bone, blood vessel, nerve, brain, liver, pancreas, spleen, kidney, lung, bronchus, respiratory track, urinary tract, gastrointestinal tract, or gynecologic tract and as a sealant for pulmonary air leaks and fistulas such as intestinal, rectal and urinary fistulas.

  17. Human Age Estimation From Skeletal and Dental Human Age Estimation From Skeletal and Dental

    E-Print Network [OSTI]

    Lucy, David

    and Section for Forensic Odontology, Oslo, Norway, for willingly providing what must be the world's most, likelihood, senescence, ageing, hard-tissue. Anthropologists and forensic scientists have found adult human

  18. A hybrid tissue-engineered heart valve

    E-Print Network [OSTI]

    Alavi, SH; Kheradvar, A

    2015-01-01

    load-bearing component of the hybrid lea?et, and it shouldin the left ventricle. Hybrid tissue was also previouslyA Hybrid Tissue-Engineered Heart Valve S. Hamed Alavi, PhD,

  19. Biomimetic electrical stimulation for cardiac tissue engineering

    E-Print Network [OSTI]

    Tandon, Nina

    2006-01-01

    A major challenge of tissue engineering is directing cells to establish the physiological structure and function of the tissue being replaced. Electrical stimulation has been used to induce synchronous contractions of ...

  20. Hydrogel cell encapsulation for tissue engineering

    E-Print Network [OSTI]

    Ling, Yibo

    2008-01-01

    The engineering of artificial tissues for restoration or replacement of organ function holds the potential to alter the landscape of medical therapeutics. In many tissue engineering approaches, cells seeded within 3D porous ...

  1. TLD skin dose measurements and acute and late effects after lumpectomy and high-dose-rate brachytherapy only for early breast cancer

    SciTech Connect (OSTI)

    Perera, Francisco [Department of Radiation Oncology, London Regional Cancer Program, London, Ontario (Canada)]. E-mail: francisco.perera@lrcc.on.ca; Chisela, Frank [Department of Radiation Oncology, Columbia St. Mary's Hospital, Milwaukee, WI (United States); Stitt, Larry [Department of Clinical Research Program, University of Western Ontario, London, Ontario (Canada); Engel, Jay [Department of Surgical Oncology, London Health Sciences Centre, London Regional Cancer Program, London, Ontario (Canada); Venkatesan, Varagur [Department of Radiation Oncology, London Regional Cancer Program, London, Ontario (Canada)

    2005-08-01

    Purpose: This report examines the relationships between measured skin doses and the acute and late skin and soft tissue changes in a pilot study of lumpectomy and high-dose-rate brachytherapy only for breast cancer. Methods and Materials: Thirty-seven of 39 women enrolled in this pilot study of high-dose-rate brachytherapy (37.2 Gy in 10 fractions b.i.d.) each had thermoluminescent dosimetry (TLD) at 5 points on the skin of the breast overlying the implant volume. Skin changes at TLD dose points and fibrosis at the lumpectomy site were documented every 6 to 12 months posttreatment using a standardized physician-rated cosmesis questionnaire. The relationships between TLD dose and acute skin reaction, pigmentation, or telangiectasia at 5 years were analyzed using the GEE algorithm and the GENMOD procedure in the SAS statistical package. Fisher's exact test was used to determine whether there were any significant associations between acute skin reaction and late pigmentation or telangiectasia or between the volumes encompassed by various isodoses and fibrosis or fat necrosis. Results: The median TLD dose per fraction (185 dose points) multiplied by 10 was 9.2 Gy. In all 37 patients, acute skin reaction Grade 1 or higher was observed at 5.9% (6 of 102) of dose points receiving 10 Gy or less vs. 44.6% (37 of 83) of dose points receiving more than 10 Gy (p < 0.0001). In 25 patients at 60 months, 1.5% telangiectasia was seen at dose points receiving 10 Gy or less (1 of 69) vs. 18% (10 of 56) telangiectasia at dose points receiving more than 10 Gy (p 0.004). Grade 1 or more pigmentation developed at 1.5% (1 of 69) of dose points receiving less than 10 Gy vs. 25% (14 of 56) of dose points receiving more than 10 Gy (p < 0.001). A Grade 1 or more acute skin reaction was also significantly associated with development of Grade 1 or more pigmentation or telangiectasia at 60 months. This association was most significant for acute reaction and telangiectasia directly over the lumpectomy site (p < 0.001). Grade 1 or more fibrosis, in 25 patients with a 60-month follow-up, occurred in 47.4% (9 of 19) of patients with a volume of 45 cm{sup 3} or less covered by the 100% isodose vs. 83.3% (5 of 6) of patients with a larger volume (p 0.180). Asymptomatic and biopsy-proven fat necrosis occurred in 5 patients. No significant differences in fat necrosis rates according to volume were detected. Conclusions: For high-dose-rate brachytherapy to the lumpectomy site, TLD skin dose was significantly related to acute skin reaction and to pigmentation and telangiectasia at 60 months. An acute skin reaction was also significantly associated with the development of telangiectasia at 60 months. TLD skin dose measurement may allow modification of the brachytherapy implant geometry (dwell times and position) to minimize late skin toxicity.

  2. Nuclear pygmy modes and the dynamics of the nuclear skin

    E-Print Network [OSTI]

    Nadia Tsoneva; Horst Lenske

    2012-11-05

    The information on pygmy resonances reveals new aspects on the isospin dynamics of the nucleus with important astrophysical consequences. In this connection, the precise knowledge of nuclear response functions plays a key role in the determination of photonuclear reactions cross sections which are of importance for the synthesis of heavy neutron-rich elements. For that purpose, a theoretical method based on density functional theory and multi-phonon approach is applied for investigations of nuclear excitations with different multipolarities and energies in stable and exotic nuclei. The possible relation of low-energy modes to the properties of neutron or proton skins is systematically investigated for isotonic and isotopic chains. Our studies of dipole and quadrupole response functions and the corresponding transition densities indicate new pygmy dipole and pygmy quadrupole resonances, describing oscillations of the nuclear skin. Also, the presence of skins is found to affect the magnetic response of nuclei.

  3. Factors Associated with the Decision to Hospitalize Emergency Department Patients with a Skin and Soft Tissue Infection

    E-Print Network [OSTI]

    2015-01-01

    management relationships that could be perceived as potential sources of bias. Antibiotic pharmaceutical

  4. Transcriptional functions of the corepressor Sin3A in skin

    E-Print Network [OSTI]

    Cox, Claire

    2013-03-12

    by the fact that disruption of their expression leads to skin disease. For example, a number of members of the S100 gene family have been observed to be up-regulated in psoriasis and skin cancer [Hoffjan and Stemmler, 2007]. Disruption of the expression... of genes in the Flg-like, LCE and SPRR families has been linked with the development of atopic dermatitis and psoriasis as well as ichthyosis vulgaris [de Guzman Strong et al., 2010]. A number of transcription factors have been implicated in the control...

  5. The role of protein solubilization in antigen removal from xenogeneic tissue for heart valve tissue engineering

    E-Print Network [OSTI]

    Athanasiou, Kyriacos

    The role of protein solubilization in antigen removal from xenogeneic tissue for heart valve tissue Extracellular matrix Heart valve tissue engineering a b s t r a c t Decellularization techniques have been of bovine pericardium (BP) for heart valve tissue engineering. Bovine pericardium following AR (BP

  6. InVERT molding for scalable control of tissue microarchitecture

    E-Print Network [OSTI]

    Ungrin, M. D.

    Complex tissues contain multiple cell types that are hierarchically organized within morphologically and functionally distinct compartments. Construction of engineered tissues with optimized tissue architecture has been ...

  7. The Impact of Biomechanics in Tissue Engineering and Regenerative Medicine

    E-Print Network [OSTI]

    Butler, David L.

    Biomechanical factors profoundly influence the processes of tissue growth, development, maintenance, degeneration, and repair. Regenerative strategies to restore damaged or diseased tissues in vivo and create living tissue ...

  8. The Effect of TNF- alpha On The Odontogenic Potential Of Human Dental Stem Cells

    E-Print Network [OSTI]

    Tseng, Edward

    2012-01-01

    dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy. Biomaterials,

  9. Human energy

    E-Print Network [OSTI]

    Sawyer, Suzana

    2010-01-01

    This is the power of human energy that Chevron neverExperience the power of Human Energy: S. Sawyer (&)s voice returns: This is the power of human energy. In

  10. Skin friction and pressure: the "footprints" of turbulence

    E-Print Network [OSTI]

    Protas, Bartosz

    been a flurry of activity in controlling both laminar and turbulent flows in certain idealized settings, and to begin to shed light on how to control fluid flow in practical engineering applications with modelSkin friction and pressure: the "footprints" of turbulence Thomas R. Bewley and Bartosz Protas Flow

  11. MOBILE PHONE USE AND TEMPORAL SKIN HEAT SENSATION

    E-Print Network [OSTI]

    Boyer, Edmond

    in the phone by the battery currents and running of the radiofrequency (RF) electronic circuits measured the temperature of the temporal skin due to GSM 1800 MHz MP radiated power (125 mW). We suppressed of the heat produced in the phone by the battery currents and running of the radiofrequency (RF) electronic

  12. ORIGINAL ARTICLE Co-habiting amphibian species harbor unique skin

    E-Print Network [OSTI]

    McKenzie, Valerie

    ; microbiome Introduction All species of plants and animals harbor assem- blages of microbes the microbiomes of different species of animals, and fewer still have examined animals in the wild. We sampled: microbemicrobe and microbehost interactions Keywords: amphibian; skin; bacteria; host specific

  13. OUTLIER ESTIMATION AND DETECTION APPLICATION TO SKIN LESION CLASSIFICATION

    E-Print Network [OSTI]

    OUTLIER ESTIMATION AND DETECTION APPLICATION TO SKIN LESION CLASSIFICATION S. Sigurdsson , J the project Signal and Image Processing for Telemedicine (SITE). Outliers are defined as an input pattern be rewritten as Դ ܵ Լ ܵ (2) where . 3. NETWORK ARCHITECTURE AND INFERENCE

  14. Criminal Skins: Tattoos and Modern Architecture in the

    E-Print Network [OSTI]

    Canales, Jimena

    Criminal Skins: Tattoos and Modern Architecture in the Work of Adolf Loos by JIMENA CANALES, `Ornament and Crime', decisively linked unornamented architecture with the culture of modernity and, in so doing, became one of the key formulations of modern architecture.1 To a great extent, the essay's force

  15. SKIN CANCER INSTITUTE THE CANCER INSTITUTES AT NORTHWESTERN MEDICINE

    E-Print Network [OSTI]

    Chisholm, Rex L.

    of specimens with unclear diagnoses sent from throughout the United States. Our pathologists are thus some than 75 percent of skin cancer deaths. It is now estimated that one in every 55 people will be diagnosed with melanoma. The incidence also is rising at a rate faster than that of the seven most common

  16. Neutron skin uncertainties of Skyrme energy density functionals

    E-Print Network [OSTI]

    M. Kortelainen; J. Erler; W. Nazarewicz; N. Birge; Y. Gao; E. Olsen

    2013-07-16

    Background: Neutron-skin thickness is an excellent indicator of isovector properties of atomic nuclei. As such, it correlates strongly with observables in finite nuclei that depend on neutron-to-proton imbalance and the nuclear symmetry energy that characterizes the equation of state of neutron-rich matter. A rich worldwide experimental program involving studies with rare isotopes, parity violating electron scattering, and astronomical observations is devoted to pinning down the isovector sector of nuclear models. Purpose: We assess the theoretical systematic and statistical uncertainties of neutron-skin thickness and relate them to the equation of state of nuclear matter, and in particular to nuclear symmetry energy parameters. Methods: We use the nuclear superfluid Density Functional Theory with several Skyrme energy density functionals and density dependent pairing. To evaluate statistical errors and their budget, we employ the statistical covariance technique. Results: We find that the errors on neutron skin increase with neutron excess. Statistical errors due to uncertain coupling constants of the density functional are found to be larger than systematic errors, the latter not exceeding 0.06 fm in most neutron-rich nuclei across the nuclear landscape. The single major source of uncertainty is the poorly determined slope L of the symmetry energy that parametrizes its density dependence. Conclusions: To provide essential constraints on the symmetry energy of the nuclear energy density functional, next-generation measurements of neutron skins are required to deliver precision better than 0.06 fm.

  17. HandWave : design and manufacture of a wearable wireless skin conductance sensor and housing

    E-Print Network [OSTI]

    Strauss, Marc D

    2005-01-01

    This thesis report details the design and manufacture of HandWave, a wearable wireless Bluetooth skin conductance sensor, and dedicated housing. The HandWave collects Electrodermal Activity (EDA) data by measuring skin ...

  18. Design and optimization of actuation mechanisms for rapid skin closure device

    E-Print Network [OSTI]

    Erickson, Andrew T. (Andrew Thomas)

    2012-01-01

    Innovative mechanism designs were explored for the actuation of critical components in a novel rapid skin closure device used to close long surgical incisions. The rapid skin closure device is designed to speed up the wound ...

  19. Tissue-equivalent torso phantom for calibration of transuranic-nuclide counting facilities

    SciTech Connect (OSTI)

    Griffith, R.V.; Anderson, A.L.; Dean, P.N.; Fisher, J.C.; Sundbeck, C.W.

    1986-01-16

    Several tissue-equivalent human-torso phantoms have been constructed for the calibration of counting systems used for in-vivo measurement of transuranic radionuclides. The phantoms contain a simulated human rib cage (in some cases, real bone) and removable model organs, and they include tissue-equivalent chest plates that can be placed over the torso to simulate people with a wide range of statures. The organs included are the lungs, liver, and tracheobronchial lymph nodes. Polyurethane with varying concentrations of added calcium was used to simulate the linear photon-attenuation properties of various human tissues, including lean muscle, adipose-muscle mixtures, cartilage, and bone. Foamed polyurethane was used to simulate lung tissue. Organs have been loaded with highly pure /sup 238/Pu, /sup 239/Pu, /sup 241/Am, and other radionuclides of interest. The validity of the phantom as a calibration standard has been checked in separate intercomparison studies using human subjects whose lungs contained a plutonium simulant. The resulting phantom calibration factors generally compared to within +-20% of the average calibration factors obtained for the human subjects.

  20. FRACTURE OF SKIN-STIFFENER INTERSECTIONS IN COMPOSITE WIND TURBINE BLADE STRUCTURES

    E-Print Network [OSTI]

    FRACTURE OF SKIN-STIFFENER INTERSECTIONS IN COMPOSITE WIND TURBINE BLADE STRUCTURES by Darrin John..................................................................4 Stiffener Design Considerations

  1. Skin Cancer: A Young Person's Disease By Lauren Duffy (B.S. Communication, Journalism '14)

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    Skin Cancer: A Young Person's Disease By Lauren Duffy (B.S. Communication, Journalism '14 is that this behavior is extremely unhealthy and risky for their bodies, specifically their skin. Skin cancer is the most common form of cancer found in young adults and second most common cancer found in adolescents

  2. How to Care for Your Wound After It's Treated With DERMABOND* Topical Skin Adhesive

    E-Print Network [OSTI]

    Kay, Mark A.

    How to Care for Your Wound After It's Treated With DERMABOND* Topical Skin Adhesive DERMABOND* Topical Skin Adhesive (2-octyl cyanoacrylate) is a sterile, liquid skin adhesive that holds wound edges If your wound is bandaged, keep the bandage dry. Replace the dressing daily until the adhesive film has

  3. Integration of contractile forces during tissue invagination

    E-Print Network [OSTI]

    Martin, Adam C.

    Contractile forces generated by the actomyosin cytoskeleton within individual cells collectively generate tissue-level force during epithelial morphogenesis. During Drosophila mesoderm invagination, pulsed actomyosin ...

  4. European trends in the frequency of original research in acne vulgaris, rosacea, dermatitis, psoriasis, skin cancer, and skin infections, 1970-2010

    E-Print Network [OSTI]

    Choi, Young M; Garcha, Jaspreet K; Wu, Jashin J

    2015-01-01

    S, Wolk K. Three decades of psoriasis research: where has itrosacea, dermatitis, psoriasis, skin cancer, and skinnotably, we found that psoriasis publications peaked around

  5. Lithium Ion Battery Performance of Silicon Nanowires With Carbon Skin

    SciTech Connect (OSTI)

    Bogart, Timothy D.; Oka, Daichi; Lu, Xiaotang; Gu, Meng; Wang, Chong M.; Korgel, Brian A.

    2013-12-06

    Silicon (Si) nanomaterials have emerged as a leading candidate for next generation lithium-ion battery anodes. However, the low electrical conductivity of Si requires the use of conductive additives in the anode film. Here we report a solution-based synthesis of Si nanowires with a conductive carbon skin. Without any conductive additive, the Si nanowire electrodes exhibited capacities of over 2000 mA h g-1 for 100 cycles when cycled at C/10 and over 1200 mA h g-1 when cycled more rapidly at 1C against Li metal.. In situ transmission electron microscopy (TEM) observation reveals that the carbon skin performs dual roles: it speeds lithiation of the Si nanowires significantly, while also constraining the final volume expansion. The present work sheds light on ways to optimize lithium battery performance by smartly tailoring the nanostructure of composition of materials based on silicon and carbon.

  6. Probing the neutron skin thickness in collective modes of excitation

    E-Print Network [OSTI]

    Nils Paar; Andrea Horvat

    2014-01-13

    Nuclear collective motion provides valuable constraint on the size of neutron-skin thickness and the properties of nuclear matter symmetry energy. By employing relativistic nuclear energy density functional (RNEDF) and covariance analysis related to $\\chi^2$ fitting of the model parameters, relevant observables are identified for dipole excitations, which strongly correlate with the neutron-skin thickness $(r_{np})$, symmetry energy at saturation density $(J)$ and slope of the symmetry energy $(L)$. Using the RNEDF framework and experimental data on pygmy dipole strength ($^{68}$Ni, $^{132}$Sn, $^{208}$Pb) and dipole polarizability ($^{208}$Pb), it is shown how the values of $J$, and $L$, and $r_{np}$ are constrained. The isotopic dependence of moments associated to dipole excitations in $^{116-136}$Sn shows that the low-energy dipole strength and polarizability in neutron-rich nuclei display strong sensitivity to the symmetry energy parameter $J$, more pronounced than in isotopes with moderate neutron-to-proton number ratios.

  7. Emergence of pygmy dipole resonances: Magic numbers and neutron skins

    E-Print Network [OSTI]

    Tsunenori Inakura; Takashi Nakatsukasa; Kazuhiro Yabana

    2011-06-18

    The pygmy dipole resonances (PDR) for even-even nuclei in 8=emerges by showing a peak of the E1 strength at energies less than 10 MeV. The E1 strength of the PDR strongly depends on the position of the Fermi level and shows a clear correlation with the occupation of the orbits with the orbital angular momenta less than 3\\hbar (l =< 2). We also found a strong correlation between the isotopic dependence of the neutron skin thickness and the pygmy dipole strength. The fraction of the energy weighted strength exhausted by the PDR and the neutron skin thickness show a linear correlation with the universal rate of about 0.2/fm.

  8. Physics of the Structural Color on the Skin of Cephalopods

    E-Print Network [OSTI]

    Gao, Meng 1981-

    2012-10-30

    by leucophore and iridophore cells, are important for cephalopod camou age; however, their scattering properties have not been very well studied. These colors are mainly due to the scattering of the speci c small scatterers inside of the cell. In this work we.... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.2 Modeled cephalpod skin with the same sequence of cells as shown in the electron micrograph: chromatophore (a), iridophore (b), and leucophore (c)). The structural color is produced by iridophore (b) and leucophore (c...

  9. Heart valve tissue engineering Frank Baaijens

    E-Print Network [OSTI]

    Stavroulakis, Georgios E.

    Heart valve tissue engineering Frank Baaijens Department of Biomedical Engineering Eindhoven University of Technology Netherlands Tissue engineered heart valves appear promising as autologous valvular functionality and durability of the heart valve relies on the strength and anisotropic properties of the valve

  10. The use of polarized light for skin cancer detecton

    E-Print Network [OSTI]

    DeLaughter, Aimee Hill

    2002-01-01

    detection system. Presented here is a method for the optical determination of cancerous tissue using polarized light. This thesis describes the development of a polarimetric imaging system including its calibration and testing. In addition, experiments...

  11. Reflective Terahertz Imaging for early diagnosis of skin burn severity

    E-Print Network [OSTI]

    TEWARI, PRIYAMVADA

    2013-01-01

    biomedical optics, (2012) P. Tewari, C.P. Kealey, J. Sung,Proc. SPIE 8261,(2012) P. Tewari, Z.D. Taylor, D. Bennett,tissues MMVR 18 (2011) P. Tewari, M. O. Culjat, Zachary

  12. Distribution of mutant cells in human skin : exploration of the fetal-juvenile mutability hypothesis

    E-Print Network [OSTI]

    Kao, Leslie E

    2009-01-01

    The multiple "hits" carcinogenesis models are extensions of the cancer incidence theory developed by researchers from Nordling (1953), Armitage-Doll (1954 and 1957), Knudson (1971), Moolgavkar and Verzon (1979), to Moolgavkar ...

  13. Steady-state directional diffuse reflectance and fluorescence of human skin

    E-Print Network [OSTI]

    Pilon, Laurent

    .3660, 170.7050, 290.7050, 300.2530. 1. Introduction Fluorescence is the physical phenomenon in which light

  14. Systematic Identification and Characterization of Novel Human Skin-Associated Genes Encoding Membrane and Secreted Proteins

    E-Print Network [OSTI]

    2013-01-01

    atopic dermatitis and psoriasis vulgaris. J Allergy Clinsystem that is active in psoriasis and promotes keratinocyteatopic dermatitis and psoriasis. Br J Dermatol 15. Kim SJ,

  15. Systematic Identification and Characterization of Novel Human Skin-Associated Genes Encoding Membrane and Secreted Proteins

    E-Print Network [OSTI]

    2013-01-01

    survey: psoriasis vulgaris (PSO), atopic dermatitis (AD),upregulated significantly in PSO, followed by AK and AD (a proinflammatory protein. PSO represents one of the major

  16. Ultraviolet a irradiation on human skin : nitric oxide mediated cardiovascular responses

    E-Print Network [OSTI]

    Liu, Donald

    2012-11-30

    Cardiovascular disease (CVD) such as hypertension and stroke are serious illnesses that impact on the lives of millions all over the world, with 972 million (26% of the worlds population) suffering from hypertension in ...

  17. Tissue fusion over non-adhering surfaces

    E-Print Network [OSTI]

    Nier, V; Duclos, G; Yevick, H G; Cochet-Escartin, O; Marcq, P; Silberzan, P

    2015-01-01

    Tissue fusion eliminates physical voids in a tissue to form a continuous structure and is central to many processes in development and repair. Fusion events in vivo, particularly in embryonic development, often involve the purse-string contraction of a pluricellular actomyosin cable at the free edge. However in vitro, adhesion of the cells to their substrate favors a closure mechanism mediated by lamellipodial protrusions, which has prevented a systematic study of the purse-string mechanism. Here, we show that monolayers can cover well-controlled mesoscopic non-adherent areas much larger than a cell size by purse-string closure and that active epithelial fluctuations are required for this process. We have formulated a simple stochastic model that includes purse-string contractility, tissue fluctuations and effective friction to qualitatively and quantitatively account for the dynamics of closure. Our data suggest that, in vivo, tissue fusion adapts to the local environment by coordinating lamellipodial protru...

  18. Systematics of nucleon density distributions and neutron skin of nuclei

    E-Print Network [OSTI]

    Seif, W M

    2015-01-01

    Proton and neutron density profiles of 760 nuclei in the mass region of A=16-304are analyzed using the Skyrme energy density for the parameter set SLy4. Simple formulae are obtained to fit the resulting radii and diffuseness data. These formulae may be useful to estimate the values of the unmeasured radii, and especially in extrapolating charge radius values for nuclei which are far from the valley of stability or to perform analytic calculations for bound and/or scattering problems. The obtained neutron and proton root-mean-square radii and the neutron skin thicknesses are in agreement with the available experimental data.

  19. In-situ measurement of skin friction and point bearing

    E-Print Network [OSTI]

    Rehmet, Joseph Don

    1970-01-01

    &e Dutch Eri& tion Cone. It is a device which makes separate but non- simultaneous measurement of skin friction and point bc riu;g during a si ati. c test. Objectives The objectives of this study are: 1. To design and fabricate several testing de... resistance to driving is: p P . (l + Jx) dynamic static (2) where P. , =- ma::imum dynamic rosie'lance, d~ j n a la I i (. static ma" imum st atic resist ance a viscous damping consLani used when i i. '' c i ' 'rnq i. he s Jj 1 I ' '. I . '* Lani...

  20. Nanotechnology in the Regeneration of Complex Tissues

    E-Print Network [OSTI]

    Cassidy, John W.

    2014-11-12

    -lithography techniques are better suited for producing regular geometries. As a bridge between tissue engineering and providing the required mechanical support, nanopatterning of orthope- dic surfaces has shown great benefit.32 However, engineering complex tissues... to reconstruct the right ventricular outflow tract during the Ross procedure. Ann Thorac Surg. 2007;84(3):729736. 64. Mann RE, Smart RG, Govoni R. The epidemiology of alcoholic liver disease. Alcohol Res Health. 2003;27(3):209219. 65. Brown KA. Liver...

  1. Deep tissue fluorescence imaging and in vivo biological applications

    E-Print Network [OSTI]

    2012-01-01

    17(11) Crosignani et al. : Deep tissue fluorescence imaging16. D. Kobat et al. , Deep tissue multiphoton microscopyV. Crosignani et al. , In vivo deep tissue fluorescence

  2. Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

    E-Print Network [OSTI]

    Annabi, Nasim

    Tissue engineering holds great promise for regeneration and repair of diseased tissues, making the development of tissue engineering scaffolds a topic of great interest in biomedical research. Because of their biocompatibility ...

  3. Phase-contrast Hounsfield units of fixated and non-fixated soft-tissue samples

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Willner, Marian; Fior, Gabriel; Marschner, Mathias; Birnbacher, Lorenz; Schock, Jonathan; Braun, Christian; Fingerle, Alexander A.; Nol, Peter B.; Rummeny, Ernst J.; Pfeiffer, Franz; et al

    2015-08-31

    X-ray phase-contrast imaging is a novel technology that achieves high soft-tissue contrast. Although its clinical impact is still under investigation, the technique may potentially improve clinical diagnostics. In conventional attenuation-based X-ray computed tomography, radiological diagnostics are quantified by Hounsfield units. Corresponding Hounsfield units for phase-contrast imaging have been recently introduced, enabling a setup-independent comparison and standardized interpretation of imaging results. Thus far, the experimental values of few tissue types have been reported; these values have been determined from fixated tissue samples. This study presents phase-contrast Hounsfield units for various types of non-fixated human soft tissues. A large variety of tissuemorespecimens ranging from adipose, muscle and connective tissues to liver, kidney and pancreas tissues were imaged by a grating interferometer with a rotating-anode X-ray tube and a photon-counting detector. In addition, we investigated the effects of formalin fixation on the quantitative phase-contrast imaging results.less

  4. On the nuclear symmetry energy and the neutron skin in neutron-rich nuclei

    E-Print Network [OSTI]

    A. E. L. Dieperink; Y. Dewulf; D. Van Neck; M. Waroquier; V. Rodin

    2003-12-10

    The symmetry energy for nuclear matter and its relation to the neutron skin in finite nuclei is discussed. The symmetry energy as a function of density obtained in a self-consistent Green function approach is presented and compared to the results of other recent theoretical approaches. A partial explanation of the linear relation between the symmetry energy and the neutron skin is proposed. The potential of several experimental methods to extract the neutron skin is examined.

  5. Poster Thur Eve 10: Partial kV CBCT, complete kV CBCT and EPID in breast treatment: a dose comparison study for skin, breasts, heart and lungs

    SciTech Connect (OSTI)

    Roussin, E; Archambault, L K; Wierzbicki, W

    2014-08-15

    The advantages of kilovoltage cone beam CT (kV CBCT) imaging over electronic portal imaging device (EPID) such as accurate 3D anatomy, soft tissue visualization, fast rigid registration and enhanced precision on patient positioning has lead to its increasing use in clinics. The benefits of this imaging technique are at the cost of increasing the dose to healthy surrounding organs. Our center has moved toward the use of daily partial rotation kV CBCT to restrict the dose to healthy tissues. This study aims to better quantify radiation doses from different image-guidance techniques such as tangential EPID, complete and partial kV CBCT for breast treatments. Cross-calibrated ionization chambers and kV calibrated Gafchromic films were used to measure the dose to the heart, lungs, breasts and skin. It was found that performing partial kV CBCT decreases the heart dose by about 36%, the lungs dose by 31%, the contralateral breast dose by 41% and the ipsilateral breast dose by 43% when compared to a full rotation CBCT. The skin dose measured for a full rotation CBCT was about 0.8 cGy for the contralateral breast and about 0.3 cGy for the ipsilateral breast. The study is still ongoing and results on skin doses for partial rotation kV CBCT as well as for tangential EPID images are upcoming.

  6. Somatic genomic variations in extra-embryonic tissues

    SciTech Connect (OSTI)

    Weier, Jingly F.; Ferlatte, Christy; Weier, Heinz-Ulli G.

    2010-05-21

    In the mature chorion, one of the membranes that exist during pregnancy between the developing fetus and mother, human placental cells form highly specialized tissues composed of mesenchyme and floating or anchoring villi. Using fluorescence in situ hybridization, we found that human invasive cytotrophoblasts isolated from anchoring villi or the uterine wall had gained individual chromosomes; however, chromosome losses were detected infrequently. With chromosomes gained in what appeared to be a chromosome-specific manner, more than half of the invasive cytotrophoblasts in normal pregnancies were found to be hyperdiploid. Interestingly, the rates of hyperdiploid cells depended not only on gestational age, but were strongly associated with the extraembryonic compartment at the fetal-maternal interface from which they were isolated. Since hyperdiploid cells showed drastically reduced DNA replication as measured by bromodeoxyuridine incorporation, we conclude that aneuploidy is a part of the normal process of placentation potentially limiting the proliferative capabilities of invasive cytotrophoblasts. Thus, under the special circumstances of human reproduction, somatic genomic variations may exert a beneficial, anti-neoplastic effect on the organism.

  7. Characterization of the homogeneous tissue mixture approximation in breast imaging dosimetry

    SciTech Connect (OSTI)

    Sechopoulos, Ioannis; Bliznakova, Kristina; Qin Xulei; Fei Baowei; Feng, Steve Si Jia

    2012-08-15

    Purpose: To compare the estimate of normalized glandular dose in mammography and breast CT imaging obtained using the actual glandular tissue distribution in the breast to that obtained using the homogeneous tissue mixture approximation. Methods: Twenty volumetric images of patient breasts were acquired with a dedicated breast CT prototype system and the voxels in the breast CT images were automatically classified into skin, adipose, and glandular tissue. The breasts in the classified images underwent simulated mechanical compression to mimic the conditions present during mammographic acquisition. The compressed thickness for each breast was set to that achieved during each patient's last screening cranio-caudal (CC) acquisition. The volumetric glandular density of each breast was computed using both the compressed and uncompressed classified images, and additional images were created in which all voxels representing adipose and glandular tissue were replaced by a homogeneous mixture of these two tissues in a proportion corresponding to each breast's volumetric glandular density. All four breast images (compressed and uncompressed; heterogeneous and homogeneous tissue) were input into Monte Carlo simulations to estimate the normalized glandular dose during mammography (compressed breasts) and dedicated breast CT (uncompressed breasts). For the mammography simulations the x-ray spectra used was that used during each patient's last screening CC acquisition. For the breast CT simulations, two x-ray spectra were used, corresponding to the x-ray spectra with the lowest and highest energies currently being used in dedicated breast CT prototype systems under clinical investigation. The resulting normalized glandular dose for the heterogeneous and homogeneous versions of each breast for each modality was compared. Results: For mammography, the normalized glandular dose based on the homogeneous tissue approximation was, on average, 27% higher than that estimated using the true heterogeneous glandular tissue distribution (Wilcoxon Signed Rank Test p= 0.00046). For dedicated breast CT, the overestimation of normalized glandular dose was, on average, 8% (49 kVp spectrum, p= 0.00045) and 4% (80 kVp spectrum, p= 0.000089). Only two cases in mammography and two cases in dedicated breast CT with a tube voltage of 49 kVp resulted in lower dose estimates for the homogeneous tissue approximation compared to the heterogeneous tissue distribution. Conclusions: The normalized glandular dose based on the homogeneous tissue mixture approximation results in a significant overestimation of dose to the imaged breast. This overestimation impacts the use of dose estimates in absolute terms, such as for risk estimates, and may impact some comparative studies, such as when modalities or techniques with different x-ray energies are used. The error introduced by the homogeneous tissue mixture approximation in higher energy x-ray modalities, such as dedicated breast CT, although statistically significant, may not be of clinical concern. Further work is required to better characterize this overestimation and potentially develop new metrics or correction factors to better estimate the true glandular dose to breasts undergoing imaging with ionizing radiation.

  8. Is the duration of skin disease visits decreasing in the united states?

    E-Print Network [OSTI]

    Davis, Scott A; Feldman, Steven R; Fleischer Jr., Alan B

    2015-01-01

    Young AC, Williford PM. Time-efficiency of nondermatologistsare generally more time-efficient in managing skin diseasein the most time-efficient manner possible. Although the

  9. An EFE model on skin-sleeve interactions during arm rotation.

    E-Print Network [OSTI]

    Xing, Malcolm M Q; Sun, Zhiguo; Pan, Ning; Zhong, Wen; Maibach, Howard I

    2006-01-01

    Sleeve Interactions During Arm Rotation Skin and garmentduring rotation of the arm. Normalized effective shearand the sleeve during the arm rotation are provided to re?

  10. Psychological Stress and skin aging: A review of possible mechanisms and potential therapies

    E-Print Network [OSTI]

    Dunn, Jeffrey H; Koo, John

    2013-01-01

    N. The grape antioxidant resveratrol for skin disorders:Therapeutic potential of resveratrol: the in vivo evidence.Kantarjian HM, et al. Resveratrol blocks interleukin-1beta-

  11. Nonstochastic effects of different energy beta emitters on pig skin

    SciTech Connect (OSTI)

    Peel, D.M.; Hopewell, J.W.; Wells, J.; Charles, M.W.

    1984-08-01

    Circular areas of pig skin from 1- to 40-mm diameter were irradiated with ..beta.. emitters of high, medium, and low energies, /sup 90/Sr, /sup 170/Tm, and /sup 147/Pm, respectively. The study provides information for radiological protection problems of localized skin exposures. During the first 16 weeks after irradiation /sup 90/Sr produced a first reaction due to epithelial cell death followed by a second reaction attributable to damage to the dermal blood vessels. /sup 170/Tm and /sup 147/Pm produced the epithelial reaction only. The epithelial dose response varied as a function of ..beta.. energy. The doses required to produce moist desquamation in 50% of 15- to 22.5-mm fields (ED/sub 50/) were 30-45 Gy from/sup 90/Sr, approx.80 Gy from /sup 170/Tm, and approx.500 Gy from /sup 147/Pm. An area effect was observed in the epithelial response to /sup 90/Sr irradiation. The ED/sub 50/ for moist desquamation ranged from approx.25 Gy for a 40-mm source to approx.450 Gy for a 1-mm source. It is also suggested that the area effects could be explained by different modes of epithelial repopulation after irradiation.

  12. Mechanical behavior of tissue simulants and soft tissues under extreme loading conditions

    E-Print Network [OSTI]

    Kalcioglu, Zeynep Ilke

    2013-01-01

    Recent developments in computer-integrated surgery and in tissue-engineered constructs necessitate advances in experimental and analytical techniques in characterizing properties of mechanically compliant materials such ...

  13. Tumor Engineering: The Other Face of Tissue Engineering

    E-Print Network [OSTI]

    Ghajar, Cyrus M

    2010-01-01

    Vacanti, J.P. Tissue engineering. Science 260, 920, 1993. 2.dynamic reciprocity: engineering three-dimensional cultureRonnov-Jessen, L. TUMOR ENGINEERING: OTHER FACE OF TISSUE

  14. Enhanced Tissue Adhesion by Increased Porosity and Surface Roughness...

    Office of Scientific and Technical Information (OSTI)

    Enhanced Tissue Adhesion by Increased Porosity and Surface Roughness of Carbon Based Biomaterials Citation Details In-Document Search Title: Enhanced Tissue Adhesion by Increased...

  15. Zo Rebecca Hunter Plasticity of the adult human brain

    E-Print Network [OSTI]

    Kallenrode, May-Britt

    Zo Rebecca Hunter Plasticity of the adult human brain and motor recovery after stroke PICS Institute of Cognitive Science #12;1 Bachelor's Thesis Plasticity of the adult human brain and motor brain and motor recovery after stroke 2 Abstract Stroke may cause a major destruction of brain tissue

  16. Distribution of phospholipase C isozymes in various rat tissues and cultured cells

    SciTech Connect (OSTI)

    Suh, P.G.; Ryu, S.H.; Choi, W.C.; Lee, K.Y.; Rhee, S.G.

    1987-05-01

    Monoclonal antibodies prepared against PLC-I or PLC-II enzyme did not cross-react with the other. Using a pair of antibodies which recognizes 2 different antigenic sites on the same molecule, radioimmunoassays were developed for the quantitation of PLC-I and PLC-II in homogenates of various tissues and cultured cells, prepared by homogenization in a 2 M KCl buffer. The contents of PLC enzymes were measured in 19 rat tissues, in human platelets and in 17 cultured cells. Results indicate that the concentration of PLC-I and PLC-II is very high in brain, PLC-I is localized mainly in brain and partly in seminal vesicles, PLC-II is found in most tissues and cells. PLC-I is highly localized even in brain: 5 different neuroblastoma did not contain PLC-I while 2 glioma and 1 astrocytoma contained significant amounts.

  17. Video Capture of Skin Motion using Calibrated Fabien DELLAS

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    3D mesh can easily be integrated and adapted in a sequence of an animated virtual human. We of virtual humans remains exhaustive and tedious. For animating virtual characters, 3D animators work similarly as drawers for car- toons, that implies hundreds of hours for only few seconds of animation

  18. Adaptive Breast Radiation Therapy Using Modeling of Tissue Mechanics: A Breast Tissue Segmentation Study

    SciTech Connect (OSTI)

    Juneja, Prabhjot; Harris, Emma J.; Kirby, Anna M.; Evans, Philip M.

    2012-11-01

    Purpose: To validate and compare the accuracy of breast tissue segmentation methods applied to computed tomography (CT) scans used for radiation therapy planning and to study the effect of tissue distribution on the segmentation accuracy for the purpose of developing models for use in adaptive breast radiation therapy. Methods and Materials: Twenty-four patients receiving postlumpectomy radiation therapy for breast cancer underwent CT imaging in prone and supine positions. The whole-breast clinical target volume was outlined. Clinical target volumes were segmented into fibroglandular and fatty tissue using the following algorithms: physical density thresholding; interactive thresholding; fuzzy c-means with 3 classes (FCM3) and 4 classes (FCM4); and k-means. The segmentation algorithms were evaluated in 2 stages: first, an approach based on the assumption that the breast composition should be the same in both prone and supine position; and second, comparison of segmentation with tissue outlines from 3 experts using the Dice similarity coefficient (DSC). Breast datasets were grouped into nonsparse and sparse fibroglandular tissue distributions according to expert assessment and used to assess the accuracy of the segmentation methods and the agreement between experts. Results: Prone and supine breast composition analysis showed differences between the methods. Validation against expert outlines found significant differences (P<.001) between FCM3 and FCM4. Fuzzy c-means with 3 classes generated segmentation results (mean DSC = 0.70) closest to the experts' outlines. There was good agreement (mean DSC = 0.85) among experts for breast tissue outlining. Segmentation accuracy and expert agreement was significantly higher (P<.005) in the nonsparse group than in the sparse group. Conclusions: The FCM3 gave the most accurate segmentation of breast tissues on CT data and could therefore be used in adaptive radiation therapy-based on tissue modeling. Breast tissue segmentation methods should be used with caution in patients with sparse fibroglandular tissue distribution.

  19. Theory of thin-skin eddy-current interaction with surface cracks N. Harfielda)

    E-Print Network [OSTI]

    Bowler, John R.

    Theory of thin-skin eddy-current interaction with surface cracks N. Harfielda) and J. R. Bowler; accepted for publication 14 July 1997 Eddy-current non-destructive evaluation is commonly performed of a typical crack. A thin-skin analysis of eddy currents is presented in which the electromagnetic fields

  20. HEAT TRANSFERS IN A DOUBLE SKIN ROOF VENTILATED BY NATURAL CONVECTION IN SUMMER TIME

    E-Print Network [OSTI]

    Boyer, Edmond

    1 HEAT TRANSFERS IN A DOUBLE SKIN ROOF VENTILATED BY NATURAL CONVECTION IN SUMMER TIME P. H or in tropical and arid countries. In this work, radiation, convection and conduction heat transfers-dimensional numerical simulation of the heat transfers through the double skin reveals the most important parameters

  1. Density dependence of the symmetry energy from neutron skin thickness in finite nuclei

    SciTech Connect (OSTI)

    Vinas, X.; Centelles, M.; Roca-Maza, X.; Warda, M.

    2012-10-20

    The density dependence of the symmetry energy, characterized by the parameter L, is studied using information provided by the neutron skin thickness in finite nuclei. An estimate of L is obtained from experimental data of antiprotonic atoms. We also discuss the ability of parity violating electron scatering to obtain information about the neutron skin thickness in {sup 208}Pb.

  2. Effects of radioactive hot particles on pig skin

    SciTech Connect (OSTI)

    Kaurin, D.G.; Baum, J.W.; Schaefer, C.W. [and others

    1997-06-01

    The purpose of these studies was to determine the incidence and severity of lesions resulting from very localized deposition of dose to skin from small (< 0.5 mm) discrete radioactive particles as produced in the work environments of nuclear reactors. Hanford mini-pigs were exposed, both on a slightly off the skin, to localized replicate doses from 0.31 to 64 Gy (averaged over 1 cm{sup 2} at 70 {mu}m depth unless noted otherwise) using Sc-46, Yb-175, Tm-170, and fissioned UC{sub 2} isotopes having maximum beta-particle energies from about 0.3 to 3 MeV. Erythema and scabs (indicating ulceration) were scored for up to 71 days post-irradiation. The responses followed normal cumulative probability distributions, and therefore, no true threshold could be defined. Hence, 10 and 50% scab incidence rates were deduced using probit analyses. The lowest dose which produced 10% incidence was about 1 Gy for Yb-175 (0.5 MeV maximum energy) beta particle exposures, and about 3 to 9 Gy for other isotopes. The histopathology of lesions was determined at several doses. Single exposures to doses as large as 1,790 Gy were also given, and results were observed for up to 144 days post-exposure. Severity of detriment was estimated by analyzing the results in terms of lesion diameter, persistence, and infection. Over 1,100 sites were exposed. Only two exposed sites became infected after doses near 5000 Gy; the lesions healed quickly on treatment. 105 refs., 145 figs., 47 tabs.

  3. Evaluation of Transverse, Bodily Tooth Movement and Its Effects on the Surrounding Hard Tissue

    E-Print Network [OSTI]

    Capps, Chad Jason

    2014-05-01

    tissue is dependent on its material composition and elastic properties.90 Muhlemann and Zander,104 who evaluated Macaca mulatta (rhesus) monkeys, and Grimm,105 who evaluated 12 year old human patients as well as cadavers, reported 24 similar findings... archwires.35 The properties of stainless steel allowed for better control in three planes of space while maintaining a moderate force level. Greater control during expansion meant further reductions in uncontrolled tipping. However, the amount and type...

  4. Nanotechnology in Biomaterials: Nanofibers in Tissue Engineering

    E-Print Network [OSTI]

    Hasrc, Vasf

    195 10 Nanotechnology in Biomaterials: Nanofibers in Tissue Engineering Deniz Yucel, Halime Kenar and Chemistry, Middle East Technical University, Ankara, Turkey 10.1 Biomaterials Devices made of synthetic. There are various de nitions of biomaterials expressed in different ways given in the literature, but more or less

  5. Inverse association between serum 25(OH) vitamin D levels and non-melanoma skin cancer in elderly men

    E-Print Network [OSTI]

    2010-01-01

    6):556562 Bikle DD (2004) Vitamin D and skin cancer. J NutrSundberg JP, Welsh J (2002) Vitamin D(3) receptor ablationbetween serum 25(OH) vitamin D levels and non-melanoma skin

  6. Activated, Not Resting, Platelets Increase Leukocyte Rolling in Murine Skin Utilizing a Distinct Set of Adhesion Molecules

    E-Print Network [OSTI]

    von Andrian, Ulrich H.

    -selectin expression was studied in 8 patients with psoriasis. A correlation between platelet P-selectin expression inflammatory skin diseases. Key words: adhesion molecules/homing/inflammation/platelets/psoriasis/skin J Invest

  7. Field, laboratory, and modeling investigation of the skin effect at wells with slotted casing, Boise Hydrogeophysical Research Site

    E-Print Network [OSTI]

    Barrash, Warren

    reserved. Keywords: Wellbore skin; Well hydraulics; Modeling; Pumping tests 1. Introduction Wellbore skin is a general term for imperfect hydraulic connection between a wellbore and the well structure and/or formationField, laboratory, and modeling investigation of the skin effect at wells with slotted casing

  8. Mutational analysis of tissue-tissue interaction required for otic placode induction in zebrafish

    E-Print Network [OSTI]

    Mendonsa, Emidio Savio

    1998-01-01

    Development of the vertebrate inner ear begins with aphics. the induction of the otic placate. Several embryonic tissues have been implicated as potential sources of otic inducing signals, including cephalic mesoderm, notochord, and hindbrain...

  9. Mechanical properties of collagen-based scaffolds for tissue regeneration

    E-Print Network [OSTI]

    Kanungo, Biraja Prasad, 1980-

    2009-01-01

    Collagen-glycosaminoglycan (CG) scaffolds for the regeneration of skin and nerve have previously been fabricated by freeze-drying a slurry containing a co-precipitate of collagen and glycosaminoglycan. Recently, mineralized ...

  10. ATHENA, the Desktop Human "Body"

    ScienceCinema (OSTI)

    Iyer, Rashi; Harris, Jennifer

    2015-01-05

    Creating surrogate human organs, coupled with insights from highly sensitive mass spectrometry technologies, a new project is on the brink of revolutionizing the way we screen new drugs and toxic agents. ATHENA, the Advanced Tissue-engineered Human Ectypal Network Analyzer project team, is developing four human organ constructs - liver, heart, lung and kidney - that are based on a significantly miniaturized platform. Each organ component will be about the size of a smartphone screen, and the whole ATHENA "body" of interconnected organs would fit neatly on a desk. "By developing this 'homo minutus,' we are stepping beyond the need for animal or Petri dish testing: There are huge benefits in developing drug and toxicity analysis systems that can mimic the response of actual human organs," said Rashi Iyer, a senior scientist at Los Alamos National Laboratory, the lead laboratory on the five-year, $19 million multi-institutional effort. The project is supported by the Defense Threat Reduction Agency (DTRA). Some 40 percent of pharmaceuticals fail their clinical trials, Iyer noted, and there are thousands of chemicals whose effects on humans are simply unknown. Providing a realistic, cost-effective and rapid screening system such as ATHENA with high-throughput capabilities could provide major benefits to the medical field, screening more accurately and offering a greater chance of clinical trial success.

  11. ATHENA, the Desktop Human "Body"

    SciTech Connect (OSTI)

    Iyer, Rashi; Harris, Jennifer

    2014-09-29

    Creating surrogate human organs, coupled with insights from highly sensitive mass spectrometry technologies, a new project is on the brink of revolutionizing the way we screen new drugs and toxic agents. ATHENA, the Advanced Tissue-engineered Human Ectypal Network Analyzer project team, is developing four human organ constructs - liver, heart, lung and kidney - that are based on a significantly miniaturized platform. Each organ component will be about the size of a smartphone screen, and the whole ATHENA "body" of interconnected organs would fit neatly on a desk. "By developing this 'homo minutus,' we are stepping beyond the need for animal or Petri dish testing: There are huge benefits in developing drug and toxicity analysis systems that can mimic the response of actual human organs," said Rashi Iyer, a senior scientist at Los Alamos National Laboratory, the lead laboratory on the five-year, $19 million multi-institutional effort. The project is supported by the Defense Threat Reduction Agency (DTRA). Some 40 percent of pharmaceuticals fail their clinical trials, Iyer noted, and there are thousands of chemicals whose effects on humans are simply unknown. Providing a realistic, cost-effective and rapid screening system such as ATHENA with high-throughput capabilities could provide major benefits to the medical field, screening more accurately and offering a greater chance of clinical trial success.

  12. The second skin approach : skin strain field analysis and mechanical counter pressure prototyping for advanced spacesuit design

    E-Print Network [OSTI]

    Bethke, Kristen (Kristen Ann)

    2005-01-01

    The primary aim of this thesis is to advance the theory of advanced locomotion mechanical counter pressure (MCP) spacesuits by studying the changes in the human body shape during joint motion. Two experiments take advantage ...

  13. Sensitivity of the electric dipole polarizability to the neutron skin thickness in {sup 208}Pb

    SciTech Connect (OSTI)

    Roca-Maza, X.; Agrawal, B. K.; Colo, G.; Nazarewicz, W.; Paar, N.; Piekarewicz, J.; Reinhard, P.-G.; Vretenar, D.

    2012-10-20

    The static dipole polarizability, {alpha}{sub D}, in {sup 208}Pb has been recently measured with highresolution via proton inelastic scattering at the Research Center for Nuclear Physics (RCNP) [1]. This observable is thought to be intimately connected with the neutron skin thickness, r{sub skin}, of the same nucleus and, more fundamentally, it is believed to be associated with the density dependence of the nuclear symmetry energy. The impact of r{sub skin} on {alpha}{sub D} in {sup 208}Pb is investigated and discussed on the basis of a large and representative set of relativistic and non-relativistic nuclear energy density functionals (EDF) [2].

  14. Precursors to radiopharmaceutical agents for tissue imaging

    DOE Patents [OSTI]

    Srivastava, Prem C. (Oak Ridge, TN); Knapp, Jr., Furn F. (Oak Ridge, TN)

    1988-01-01

    A class of radiolabeled compounds to be used in tissue imaging that exhibits rapid brain uptake, good brain:blood radioactivity ratios, and long retention times. The imaging agents are more specifically radioiodinated aromatic amines attached to dihydropyridine carriers, that exhibit heart as well as brain specificity. In addition to the radiolabeled compounds, classes of compounds are also described that are used as precursors and intermediates in the preparation of the imaging agents.

  15. Predicting the Occurrence of Cosmetic Defects in Automotive Skin Panels

    SciTech Connect (OSTI)

    Hazra, S.; Williams, D.; Roy, R.; Aylmore, R.; Allen, M.; Hollingdale, D.

    2011-05-04

    The appearance of defects such as 'hollows' and 'shock lines' can affect the perceived quality and attractiveness of automotive skin panels. These defects are the result of the stamping process and appear as small, localized deviations from the intended styling of the panels. Despite their size, they become visually apparent after the application of paint and the perceived quality of a panel may become unacceptable. Considerable time is then dedicated to minimizing their occurrence through tool modifications. This paper will investigate the use of the wavelet transform as a tool to analyze physically measured panels. The transform has two key aspects. The first is its ability to distinguish small scale local defects from large scale styling curvature. The second is its ability to characterize the shape of a defect in terms of its wavelength and a 'correlation value'. The two features of the transform enable it to be used as a tool for locating and predicting the severity of defects. The paper will describe the transform and illustrate its application on test cases.

  16. Method for preparing dosimeter for measuring skin dose

    DOE Patents [OSTI]

    Jones, Donald E. (Idaho Falls, ID); Parker, DeRay (Idaho Falls, ID); Boren, Paul R. (Idaho Falls, ID)

    1982-01-01

    A personnel dosimeter includes a plurality of compartments containing thermoluminescent dosimeter phosphors for registering radiation dose absorbed in the wearer's sensitive skin layer and for registering more deeply penetrating radiation. Two of the phosphor compartments communicate with thin windows of different thicknesses to obtain a ratio of shallowly penetrating radiation, e.g. beta. A third phosphor is disposed within a compartment communicating with a window of substantially greater thickness than the windows of the first two compartments for estimating the more deeply penetrating radiation dose. By selecting certain phosphors that are insensitive to neutrons and by loading the holder material with neutron-absorbing elements, energetic neutron dose can be estimated separately from other radiation dose. This invention also involves a method of injection molding of dosimeter holders with thin windows of consistent thickness at the corresponding compartments of different holders. This is achieved through use of a die insert having the thin window of precision thickness in place prior to the injection molding step.

  17. Dosimeter for measuring skin dose and more deeply penetrating radiation

    DOE Patents [OSTI]

    Jones, Donald E. (Idaho Falls, ID); Parker, DeRay (Idaho Falls, ID); Boren, Paul R. (Idaho Falls, ID)

    1981-01-01

    A personnel dosimeter includes a plurality of compartments containing thermoluminescent dosimeter phosphors for registering radiation dose absorbed in the wearer's sensitive skin layer and for registering more deeply penetrating radiation. Two of the phosphor compartments communicate with thin windows of different thicknesses to obtain a ratio of shallowly penetrating radiation, e.g. beta. A third phosphor is disposed within a compartment communicating with a window of substantially greater thickness than the windows of the first two compartments for estimating the more deeply penetrating radiation dose. By selecting certain phosphors that are insensitive to neutrons and by loading the holder material with netruon-absorbing elements, energetic neutron dose can be estimated separately from other radiation dose. This invention also involves a method of injection molding of dosimeter holders with thin windows of consistent thickness at the corresponding compartments of different holders. This is achieved through use of a die insert having the thin window of precision thickness in place prior to the injection molding step.

  18. Colloquium: Mechanical formalisms for tissue dynamics

    E-Print Network [OSTI]

    Sham Tlili; Cyprien Gay; Francois Graner; Philippe Marcq; Franois Molino; Pierre Saramito

    2015-09-29

    The understanding of morphogenesis in living organisms has been renewed by tremendous progressin experimental techniques that provide access to cell-scale, quantitative information both on theshapes of cells within tissues and on the genes being expressed. This information suggests that ourunderstanding of the respective contributions of gene expression and mechanics, and of their crucialentanglement, will soon leap forward. Biomechanics increasingly benefits from models, which assistthe design and interpretation of experiments, point out the main ingredients and assumptions, andultimately lead to predictions. The newly accessible local information thus calls for a reflectionon how to select suitable classes of mechanical models. We review both mechanical ingredientssuggested by the current knowledge of tissue behaviour, and modelling methods that can helpgenerate a rheological diagram or a constitutive equation. We distinguish cell scale ("intra-cell")and tissue scale ("inter-cell") contributions. We recall the mathematical framework developpedfor continuum materials and explain how to transform a constitutive equation into a set of partialdifferential equations amenable to numerical resolution. We show that when plastic behaviour isrelevant, the dissipation function formalism appears appropriate to generate constitutive equations;its variational nature facilitates numerical implementation, and we discuss adaptations needed in thecase of large deformations. The present article gathers theoretical methods that can readily enhancethe significance of the data to be extracted from recent or future high throughput biomechanicalexperiments.

  19. p27{sup Kip1} inhibits tissue factor expression

    SciTech Connect (OSTI)

    Breitenstein, Alexander, E-mail: alexander.breitenstein@usz.ch [Cardiology, University Heart Center, University Hospital Zurich (Switzerland) [Cardiology, University Heart Center, University Hospital Zurich (Switzerland); Cardiovascular Research, Physiology Institute, University of Zurich (Switzerland); Center for Integrative Human Physiology (ZHIP), University of Zurich (Switzerland); Akhmedov, Alexander; Camici, Giovanni G.; Lscher, Thomas F.; Tanner, Felix C. [Cardiology, University Heart Center, University Hospital Zurich (Switzerland) [Cardiology, University Heart Center, University Hospital Zurich (Switzerland); Cardiovascular Research, Physiology Institute, University of Zurich (Switzerland); Center for Integrative Human Physiology (ZHIP), University of Zurich (Switzerland)

    2013-10-04

    Highlights: p27{sup Kip1}regulates the expression of tissue factor at the transcriptional level. This inhibitory effect of p27{sup Kip1} is independently of its cell regulatory action. The current study provides new insights into a pleiotrophic function of p27{sup Kip1}. -- Abstract: Background: The cyclin-dependent kinase inhibitor (CDKI) p27{sup Kip1} regulates cell proliferation and thus inhibits atherosclerosis and vascular remodeling. Expression of tissue factor (TF), the key initator of the coagulation cascade, is associated with atherosclerosis. Yet, it has not been studied whether p27{sup Kip1} influences the expression of TF. Methods and results: p27{sup Kip1} overexpression in human aortic endothelial cells was achieved by adenoviral transfection. Cells were rendered quiescent for 24 h in 0.5% fetal-calf serum. After stimulation with TNF-? (5 ng/ml), TF protein expression and activity was significantly reduced (n = 4; P < 0.001) in cells transfected with p27{sup Kip1}. In line with this, p27{sup Kip1} overexpression reduced cytokine-induced TF mRNA expression (n = 4; P < 0.01) and TF promotor activity (n = 4; P < 0.05). In contrast, activation of the MAP kinases p38, ERK and JNK was not affected by p27{sup Kip1} overexpression. Conclusion: This in vitro study suggests that p27{sup Kip1} inhibits TF expression at the transcriptional level. These data indicate an interaction between p27{sup Kip1} and TF in important pathological alterations such as atherosclerosis and vascular remodeling.

  20. Cell-Graph Mining for Breast Tissue Modelling and Classification

    E-Print Network [OSTI]

    Bystroff, Chris

    metrics for tissue characteriza- tion and classification. We segmented the digital images of histopaCell-Graph Mining for Breast Tissue Modelling and Classification C.C agatay Bilgin a, C igdem

  1. Decellularized cartilage as a chondroinductive material for cartilage tissue engineering

    E-Print Network [OSTI]

    Renth, Amanda

    2013-12-31

    Cartilage defects, whether caused by osteoarthritis, joint trauma, or other disease, have provoked a wide variety of tissue engineering scaffold strategies in recent years. Traditionally, cartilage tissue engineering scaffolds have utilized...

  2. Optical Mapping of Impulse Propagation in Engineered Cardiac Tissue

    E-Print Network [OSTI]

    Radisic, Milica

    Cardiac tissue engineering has a potential to provide functional, synchronously contractile tissue constructs for heart repair, and for studies of development and disease using in vivolike yet controllable in vitro settings. ...

  3. Tuning adhesion failure strength for tissue-specific applications

    E-Print Network [OSTI]

    Artzi, Natalie

    Soft tissue adhesives are employed to repair and seal many different organs, which range in both tissue surface chemistry and mechanical challenges during organ function. This complexity motivates the development of tunable ...

  4. High-Throughput Single-Cell Manipulation in Brain Tissue

    E-Print Network [OSTI]

    Steinmeyer, Joseph Daly

    The complexity of neurons and neuronal circuits in brain tissue requires the genetic manipulation, labeling, and tracking of single cells. However, current methods for manipulating cells in brain tissue are limited to ...

  5. Collisional, magnetic, and nonlinear skin effect in radio-frequency plasmas Francis F. Chen

    E-Print Network [OSTI]

    Chen, Francis F.

    . CLASSICAL VS ANOMALOUS SKIN EFFECT Inductively coupled plasmas ICPs are commonly used in plasma processing.11 The experiments of Godyak et al.36,9 were performed with a spiral ``stove-top'' antenna, which

  6. IN FOCUS: FUTURE OF BIOSENSORS -ARTICLE Epidermal Differential Impedance Sensor for Conformal Skin

    E-Print Network [OSTI]

    Rogers, John A.

    IN FOCUS: FUTURE OF BIOSENSORS - ARTICLE Epidermal Differential Impedance Sensor for Conformal Skin requirements This article is part of the Topical Collection ``In Focus: Future of Biosensors''. X. Huang W

  7. Development and Construction of Bioclimatic Double Skin Active Facade for Hot and Humid Climate of UAE

    E-Print Network [OSTI]

    Karbor, R. G.; Mohamed, I.

    2010-01-01

    become still more advanced. The present paper deals with the development and construction of mechanically ventilated double skin facade with HVAC integration for hot and humid climate like UAE. A case study is presented, illustrating potential benefits...

  8. Design Considerations for Double-Skin Facades in Hot and Humid Climates

    E-Print Network [OSTI]

    Haase, M.; Amato, A.

    2006-01-01

    Thermal building simulations (TRNSYS) were linked to nodal airflow network simulations (COMIS) for a ventilated double-skin facade performance calculation and overall energy consumption for office building facades. Simulation results show good...

  9. Meeting report for the 1st skin microbiota workshop, Boulder, CO October 15-16 2012

    E-Print Network [OSTI]

    Gilbert, Jack A.

    This report details the outcome of the 1st Skin Microbiota Workshop, Boulder, CO, held on October 15th-16th 2012. The workshop was arranged to bring Department of Defense personnel together with experts in microbial ecology, ...

  10. A Systematic Study of Matrix Acidizing Treatments Using Skin Monitoring Method

    E-Print Network [OSTI]

    Pandya, Nimish

    2012-07-16

    The goal of this work was to evaluate matrix acidizing treatments of vertical and horizontal wells in carbonate reservoirs. Twenty field cases for acidizing treatments were analyzed by evaluating the skin factor evolution from on-site rate/pressure...

  11. Has a thick neutron skin in ${}^{208}$Pb been ruled out?

    E-Print Network [OSTI]

    Fattoyev, F J

    2013-01-01

    The Lead Radius Experiment (PREX) has provided the first model-independent evidence in favor of a neutron-rich skin in ${}^{208}$Pb. Although the error bars are large, the reported large central value of 0.33\\,fm is particularly intriguing. To test whether such a thick neutron-skin in ${}^{208}$Pb is already incompatible with laboratory experiments or astrophysical observations, we employ relativistic models with neutron-skin thickness in ${}^{208}$Pb ranging from 0.16 to 0.33 fm to compute ground state properties of finite nuclei, their collective monopole and dipole response, and mass-{\\sl vs}-radius relations for neutron stars. No compelling reason was found to rule out models with large neutron skins in ${}^{208}$Pb from the set of observables considered in this work.

  12. REFLEXIVE COLLISION RESPONSE WITH VIRTUAL SKIN Roadmap Planning Meets Reinforcement Learning

    E-Print Network [OSTI]

    Frster, Alexander

    REFLEXIVE COLLISION RESPONSE WITH VIRTUAL SKIN Roadmap Planning Meets Reinforcement Learning Svizzera italiana, CH-6928 Manno-Lugano Keywords: Roadmap Planning: Reinforcement Learning: Collision to a changing environment, but not both. This work proposes a simple integration of roadmap planning

  13. Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells

    E-Print Network [OSTI]

    Iyer, Dharini; Gambardella, Laure; Bernard, William G.; Serrano, Felipe; Mascetti, Victoria L.; Pedersen, Roger A.; Talasila, Amarnath; Sinha, Sanjay

    2015-03-26

    and its potential ability to initiate myocardial repair in injured adult tissues. Here, we describe a chemically defined method for generating epicardium and epicardium-derived smooth muscle cells (EPI-SMCs) and CFs from human pluripotent stem cells (HPSCs...

  14. Role for oestrogen in dynamic interactions between cell types within the human endometrium

    E-Print Network [OSTI]

    Gibson, Douglas Alistair

    2012-11-30

    The human endometrium is a complex multicellular tissue, located within the cavity of the uterus. Its luminal surface is defined by a layer of epithelial cells supported on a multicellular stroma containing fibroblasts, ...

  15. Original Paper CellsTissues Organs 2004;178:3347

    E-Print Network [OSTI]

    Burnstock, Geoffrey

    phosphate-buffered saline #12;Katugampola/BurnstockCells Tissues Organs 2004;178:334734 Introduction

  16. Fusing Tissue Engineering and Systems Biology Toward Fulfilling Their Promise

    E-Print Network [OSTI]

    ,45,60 mammary gland morphogenesis and oncogenesis,8,53 lymphoid tissue neogenesis,26,63 and stem cell

  17. Theoretical evaluation on burn injury of human respiratory tract due to inhalation of

    E-Print Network [OSTI]

    Zhang, Jun

    at tissue temperature (kPa) mQ Metabolic rate of tissue (W/m3 ) R Ideal gas constant (J/molK) Re Reynolds1 Theoretical evaluation on burn injury of human respiratory tract due to inhalation of hot gas to predict the thermal impact of inhaled hot air during the early stage of fires. Influences of individual

  18. Evaluation of the inhibition of skin matrix metalloproteinases by pothomorphe umbellata root extract

    E-Print Network [OSTI]

    Ropke, Cristina D.

    2006-10-25

    . 2003 3Ropke et al., Clin. Exp. Dermatol., 2005, 4Ropke et al., Photochem. Photobiol. 2006 in vivo results ? P. umbellata extract was able to reduce the incidence of visible and histological skin alterations in chronically UV- irradiated mice.... 2003 3Ropke et al., Clin. Exp. Dermatol., 2005, 4Ropke et al., Photochem. Photobiol. 2006 in vivo results ? P. umbellata extract was able to reduce the incidence of visible and histological skin alterations in chronically UV- irradiated mice...

  19. Oncogenic action of beta, proton, alpha and electron radiation on the rat skin

    SciTech Connect (OSTI)

    Burns, F.J.

    1980-01-01

    Rat skin is being utilized as an empirical model for testing dose and time related aspects of the oncogenic action of ionizing radiation, ultraviolet light, and polycyclic aromatic hydrocarbons. Molecular lesions in the skin DNA, including, strand breaks and thymine dimers, are being measured and compared to tumor induction. The induction and repair kinetics of molcular lesions are being compared to split dose repair. Modifiers and radiosensitizers are being utilized to test specific aspects of a chromosome breakage theory of radiation oncogenesis.

  20. Dose profiles through the dermis for on and off-skin hot particle exposures

    E-Print Network [OSTI]

    Shaw, Kimberly Rochelle

    1993-01-01

    reports measurements of depth-dose profiles for on- and off-skin hot particle exposures using radiochromic dye film. Dose profiles from both a "Co hot particle, and activated depleted uranium oxide microspheres were measured with the film. Exposures... 24 27 32 TABLE OF CONTENTS Icontinued) Page RESULTS 34 ' Co On-Contact Exposures 34 Co Exposures Through Protective Clothing ~Co Off-Skin Exposures Uranium Microsphere On-Contact Exposures 45 49 Uranium Microsphere Exposures Through...

  1. EXPLORING THE POTENTIAL OF SHORT-TIME FOURIER TRANSFORMS FOR ANALYZING SKIN CONDUCTANCE AND PUPILLOMETRY IN REAL-TIME APPLICATIONS

    SciTech Connect (OSTI)

    Roger Lew; Brian P. Dyre; Steffen Werner; Jeffrey C. Joe; Brian Wotring; Tuan Tran

    2008-09-01

    The development of real-time predictors of mental workload is critical for the practical application of augmented cognition to human-machine systems. This paper explores a novel method based on a short-time Fourier transform (STFT) for analyzing galvanic skin conductance (SC) and pupillometry time-series data to extract estimates of mental workload with temporal bandwidth high-enough to be useful for augmented cognition applications. We tested the method in the context of a process control task based on the DURESS simulation developed by Vincente and Pawlak (1994; ported to Java by Cosentino,& Ross, 1999). SC, pupil dilation, blink rate, and visual scanning patterns were measured for four participants actively engaged in controlling the simulation. Fault events were introduced that required participants to diagnose errors and make control adjustments to keep the simulator operating within a target range. We were interested in whether the STFT of these measures would produce visible effects of the increase in mental workload and stress associated with these events. Graphical exploratory data analysis of the STFT showed visible increases in the power spectrum across a range of frequencies directly following fault events. We believe this approach shows potential as a relatively unobtrusive, low-cost, high bandwidth measure of mental workload that could be particularly useful for the application of augmented cognition to human-machine systems.

  2. NON-INVASIVE MEASUREMENT OF DEEP TISSUE TEMPERATURE CHANGES

    E-Print Network [OSTI]

    Yodh, Arjun G.

    deep tissue temperature using subtle spectral features of the water peak at 975 nm.3 A signi- ging (DOSI) to measure deep tissue temperature, using spectral features of the water absorption peakNON-INVASIVE MEASUREMENT OF DEEP TISSUE TEMPERATURE CHANGES CAUSED BY APOPTOSIS DURING BREAST

  3. Tissue tectonics: morphogenetic strain rates, cell shape change and intercalation

    E-Print Network [OSTI]

    Cai, Long

    such a kinematic framework, bridging cell and tissue behaviors at an intermediate, mesoscopic, level of cell spatiotemporally in three models of tissue morphogenesis, gaining insight into morphogenetic mechanisms. Our analysis and continuum field theories11 and extended these methods for tissues composed of discrete cells

  4. Norathyriol Suppresses Skin Cancers Induced by Solar Ultraviolet Radiation by Targeting ERK Kinases

    SciTech Connect (OSTI)

    Li, Jixia; Malakhova, Margarita; Mottamal, Madhusoodanan; Reddy, Kanamata; Kurinov, Igor; Carper, Andria; Langfald, Alyssa; Oi, Naomi; Kim, Myoung Ok; Zhu, Feng; Sosa, Carlos P.; Zhou, Keyuan; Bode, Ann M.; Dong, Zigang

    2012-06-27

    Ultraviolet (UV) irradiation is the leading factor in the development of skin cancer, prompting great interest in chemopreventive agents for this disease. In this study, we report the discovery of norathyriol, a plant-derived chemopreventive compound identified through an in silico virtual screening of the Chinese Medicine Library. Norathyriol is a metabolite of mangiferin found in mango, Hypericum elegans, and Tripterospermum lanceolatum and is known to have anticancer activity. Mechanistic investigations determined that norathyriol acted as an inhibitor of extracellular signal-regulated kinase (ERK)1/2 activity to attenuate UVB-induced phosphorylation in mitogen-activated protein kinases signaling cascades. We confirmed the direct and specific binding of norathyriol with ERK2 through a cocrystal structural analysis. The xanthone moiety in norathyriol acted as an adenine mimetic to anchor the compound by hydrogen bonds to the hinge region of the protein ATP-binding site on ERK2. Norathyriol inhibited in vitro cell growth in mouse skin epidermal JB6 P+ cells at the level of G{sub 2}-M phase arrest. In mouse skin tumorigenesis assays, norathyriol significantly suppressed solar UV-induced skin carcinogenesis. Further analysis indicated that norathyriol mediates its chemopreventive activity by inhibiting the ERK-dependent activity of transcriptional factors AP-1 and NF-{kappa}B during UV-induced skin carcinogenesis. Taken together, our results identify norathyriol as a safe new chemopreventive agent that is highly effective against development of UV-induced skin cancer.

  5. ChIP-seq Accurately Predicts Tissue-Specific Activity of Enhancers

    SciTech Connect (OSTI)

    Visel, Axel; Blow, Matthew J.; Li, Zirong; Zhang, Tao; Akiyama, Jennifer A.; Holt, Amy; Plajzer-Frick, Ingrid; Shoukry, Malak; Wright, Crystal; Chen, Feng; Afzal, Veena; Ren, Bing; Rubin, Edward M.; Pennacchio, Len A.

    2009-02-01

    A major yet unresolved quest in decoding the human genome is the identification of the regulatory sequences that control the spatial and temporal expression of genes. Distant-acting transcriptional enhancers are particularly challenging to uncover since they are scattered amongst the vast non-coding portion of the genome. Evolutionary sequence constraint can facilitate the discovery of enhancers, but fails to predict when and where they are active in vivo. Here, we performed chromatin immunoprecipitation with the enhancer-associated protein p300, followed by massively-parallel sequencing, to map several thousand in vivo binding sites of p300 in mouse embryonic forebrain, midbrain, and limb tissue. We tested 86 of these sequences in a transgenic mouse assay, which in nearly all cases revealed reproducible enhancer activity in those tissues predicted by p300 binding. Our results indicate that in vivo mapping of p300 binding is a highly accurate means for identifying enhancers and their associated activities and suggest that such datasets will be useful to study the role of tissue-specific enhancers in human biology and disease on a genome-wide scale.

  6. SU-E-I-53: Variation in Measurements of Breast Skin Thickness Obtained Using Different Imaging Modalities

    SciTech Connect (OSTI)

    Nguyen, U; Kumaraswamy, N; Markey, M

    2014-06-01

    Purpose: To investigate variation in measurements of breast skin thickness obtained using different imaging modalities, including mammography, computed tomography (CT), ultrasound, and magnetic resonance imaging (MRI). Methods: Breast skin thicknesses as measured by mammography, CT, ultrasound, and MRI were compared. Mammographic measurements of skin thickness were obtained from published studies that utilized standard positioning (upright) and compression. CT measurements of skin thickness were obtained from a published study of a prototype breast CT scanner in which the women were in the prone position and the breast was uncompressed. Dermatological ultrasound exams of the breast skin were conducted at our institution, with the subjects in the upright position and the breast uncompressed. Breast skin thickness was calculated from breast MRI exams at our institution, with the patient in the prone position and the breast uncompressed. Results: T tests for independent samples demonstrated significant differences in the mean breast skin thickness as measured by different imaging modalities. Repeated measures ANOVA revealed significant differences in breast skin thickness across different quadrants of the breast for some modalities. Conclusion: The measurement of breast skin thickness is significantly different across different imaging modalities. Differences in the amount of compression and differences in patient positioning are possible reasons why measurements of breast skin thickness vary by modality.

  7. Electronic equilibrium as a function of depth in tissue from Cobalt-60 point source exposures

    E-Print Network [OSTI]

    Myrick, Jo Ann

    1994-01-01

    . Skin exposure can arise from both the beta and gamma components of radioactive particles and gamma radiation can contribute significantly to skin doses. The gamma component of dose increases dramatically when layers of protective clothing are interposed...

  8. WHAT IS PLANT TISSUE CULTURE? Plant tissue culture involves the growth of plant cells, tissues or segments for purposes such as

    E-Print Network [OSTI]

    Durako, Michael J.

    or segments for purposes such as generating or cloning large amounts of new cells, tissues or plants; to study of generating exact and multiple copies of a parent plant, Cloning can be done by making cuttings, grafts pieces of tissues such as axillary buds, tubers or rhizomes for rapid cloning or generation of new plants

  9. The Effect of the iBEAM Evo Carbon Fiber Tabletop on Skin Sparing

    SciTech Connect (OSTI)

    Simpson, John B. Godwin, Guy A.

    2011-10-01

    Replicating the attenuation properties of the treatment tabletop are of primary importance for accurate treatment planning; however, the effect of the tabletop on the skin-sparing properties of x-rays can be overlooked. Under some conditions, the reaction of skin to the radiation can be so serious as to be the dose-limiting organ for radiotherapy treatment. Hence, an understanding of the magnitude of the reduction in skin sparing is important. Because of the development of image-guided radiotherapy, modern tabletops have been developed without the use of metal supports that otherwise provided the necessary level of rigidity. Rigidity is instead provided by compressed foam within a carbon-fiber shell, which, although it provides artefact-free imaging and high levels of rigidity, has an adverse affect on the dose in the build-up region. Representative of this type is the iBEAM evo tabletop, whose effect on the skin dose was determined at 6-MV, 10-MV, and 18-MV x-rays. Skin dose was found to increase by 60-70% owing to the tabletop, with the effect increasing with field size and decreasing with energy. By considering an endpoint of erythema, a radiobiological advantage of selecting 10 MV over 6 MV for applicable treatments was demonstrated.

  10. Human Subjects Section 6. Protection of Human

    E-Print Network [OSTI]

    Heller, Barbara

    Human Subjects Section 6. Protection of Human Subjects This section is required for applicants answering "yes" to the question "Are human subjects involved?" on the R&R Other Project Information form subjects applicants must provide a justification in this section for the claim that no human subjects

  11. ReseaRch at the University of Maryland Tissue Engineering Research

    E-Print Network [OSTI]

    Hill, Wendell T.

    ReseaRch at the University of Maryland Tissue Engineering Research Tissue engineering relies on biomaterials science the interface between cell biology and engineering applications. Scientists-joint tissues, among other engineered bio- applications. Tissue engineering uses the remarkable properties

  12. In vivo tissue enzyme activities in the rosy barb (Barbus conchonius Hamilton) experimentally exposed to lead

    SciTech Connect (OSTI)

    Gill, T.S. ); Tewari, H.; Pande, J.; Lal, S. )

    1991-12-01

    Lead (Pb) is biologically nonessential and if present in excessive levels in the body, it can cause clinical disorders both in humans and animals. Pathologies associated with experimental Pb poisoning have been described in fishes, and several markers have been utilized to monitor effects of short- and long-term exposures. The specific aim of this study was to examine the effects of acute Pb poisoning on the enzymes concerned with membrane transport, neurotransmission, and energy metabolism in selected tissues of the rosy barb, Barbus conchonius, a freshwater fish.

  13. Total skin electron beam therapy using an inclinable couch on motorized table and a compensating filter

    SciTech Connect (OSTI)

    Fuse, H.; Suzuki, K.; Shida, K.; Takahashi, H.; Kobayashi, D.; Seki, M.; Mori, Y.; Sakae, T.; Isobe, T.; Okumura, T.; Sakurai, H.

    2014-06-15

    Total skin electron beam is a specialized technique that involves irradiating the entire skin from the skin surface to only a few millimetres in depth. In the Stanford technique, the patient is in a standing position and six different directional positions are used during treatment. Our technique uses large electron beams in six directions with an inclinable couch on motorized table and a compensating filter was also used to spread the electron beam and move its intensity peak. Dose uniformity measurements were performed using Gafchromic films which indicated that the surface dose was 2.04 0.05 Gy. This technique can ensure the dose reproducibility because the patient is fixed in place using an inclinable couch on a motorized table.

  14. Techniques to quantify vascular ingrowth in soft tissue biomaterials

    E-Print Network [OSTI]

    Estridge, Trudy Donette

    1988-01-01

    of tissue ingrowth into four distinct regions at one month post- operatlvely. Direct measurement, however, was very labor intensive, In E&periment III, all three quantification methods were 4 4 performed on plain Dacron velour and lactic acid treated... sections (8), and a sanewhat novel approach of studying vascular casts of the vascular supply. Previously, the only method used to study the vasculature ln porous soft tissue implants was the pathological method of looking at tissue sections. Even using...

  15. Iron is the Key to Preserving Dinosaur Soft Tissue

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the iron associated with fossil tissues, which occurred primarily as the mineral goethite. They then employed experiments to show that iron, derived from hemoglobin lysate,...

  16. ACES: Evaluation of Tissue Response to Inhaled 2007-Compliant...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Phase 3 of the ACES study produced minimal inflammatory and tissue remodeling in their lungs and no soot accumulation in macrophages. deer12shaikh.pdf More Documents &...

  17. Tissue Engineering by Integrating Stem Cells and Polymeric Biomaterials

    E-Print Network [OSTI]

    Fisher, Frank

    Tissue Engineering by Integrating Stem Cells and Polymeric Biomaterials Wednesday September 14 Award and Yasuda Award. His research interest is in the interface of biomaterials and stem cells

  18. microRNA Alterations Driving Acute and Late Stages of Radiation-Induced Fibrosis in a Murine Skin Model

    SciTech Connect (OSTI)

    Simone, Brittany A.; Ly, David; Savage, Jason E.; Hewitt, Stephen M.; Dan, Tu D.; Ylaya, Kris; Shankavaram, Uma; Lim, Meng; Jin, Lianjin; Camphausen, Kevin; Mitchell, James B.; Simone, Nicole L.

    2014-09-01

    Purpose: Although ionizing radiation is critical in treating cancer, radiation-induced fibrosis (RIF) can have a devastating impact on patients' quality of life. The molecular changes leading to radiation-induced fibrosis must be elucidated so that novel treatments can be designed. Methods and Materials: To determine whether microRNAs (miRs) could be responsible for RIF, the fibrotic process was induced in the right hind legs of 9-week old CH3 mice by a single-fraction dose of irradiation to 35Gy, and the left leg served as an unirradiated control. Fibrosis was quantified by measurements of leg length compared with control leg length. By 120days after irradiation, the irradiated legs were 20% (P=.013) shorter on average than were the control legs. Results: Tissue analysis was done on muscle, skin, and subcutaneous tissue from irradiated and control legs. Fibrosis was noted on both gross and histologic examination by use of a pentachrome stain. Microarrays were performed at various times after irradiation, including 7days, 14days, 50days, 90days, and 120days after irradiation. miR-15a, miR-21, miR-30a, and miR-34a were the miRs with the most significant alteration by array with miR-34a, proving most significant on confirmation by reverse transcriptase polymerase chain reaction, c-Met, a known effector of fibrosis and downstream molecule of miR-34a, was evaluated by use of 2cell lines: HCT116 and 1522. The cell lines were exposed to various stressors to induce miR changes, specifically ionizing radiation. Additionally, invitro transfections with pre-miRs and anti-miRs confirmed the relationship of miR-34a and c-Met. Conclusions: Our data demonstrate an inverse relationship with miR-34a and c-Met; the upregulation of miR-34a in RIF causes inhibition of c-Met production. miRs may play a role in RIF; in particular, miR-34a should be investigated as a potential target to prevent or treat this devastating side effect of irradiation.

  19. Generation of a suite of 3D computer-generated breast phantoms from a limited set of human subject data

    SciTech Connect (OSTI)

    Hsu, Christina M. L. [Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 and Carl E. Ravin Advanced Imaging Laboratories, Duke University Medical Center, Durham, North Carolina 27705 (United States); Palmeri, Mark L. [Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 (United States); Department of Anesthesiology, Duke University Medical Center, Durham, North Carolina 27710 (United States); Segars, W. Paul [Carl E. Ravin Advanced Imaging Laboratories, Duke University Medical Center, Durham, North Carolina 27705 (United States); Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710 (United States); Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 (United States); Medical Physics Graduate Program, Duke University, Durham, North Carolina 27705 (United States); Veress, Alexander I. [Department of Mechanical Engineering, University of Washington, Seattle, Washington 98195 (United States); Dobbins, James T. III [Carl E. Ravin Advanced Imaging Laboratories, Duke University Medical Center, Durham, North Carolina 27705 (United States); Department of Radiology, Duke University Medical Center, Durham, North Carolina 27710 (United States); Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 (United States); Medical Physics Graduate Program, Duke University, Durham, North Carolina 27705 (United States); Department of Physics, Duke University, Durham, North Carolina 27708 (United States)

    2013-04-15

    Purpose: The authors previously reported on a three-dimensional computer-generated breast phantom, based on empirical human image data, including a realistic finite-element based compression model that was capable of simulating multimodality imaging data. The computerized breast phantoms are a hybrid of two phantom generation techniques, combining empirical breast CT (bCT) data with flexible computer graphics techniques. However, to date, these phantoms have been based on single human subjects. In this paper, the authors report on a new method to generate multiple phantoms, simulating additional subjects from the limited set of original dedicated breast CT data. The authors developed an image morphing technique to construct new phantoms by gradually transitioning between two human subject datasets, with the potential to generate hundreds of additional pseudoindependent phantoms from the limited bCT cases. The authors conducted a preliminary subjective assessment with a limited number of observers (n= 4) to illustrate how realistic the simulated images generated with the pseudoindependent phantoms appeared. Methods: Several mesh-based geometric transformations were developed to generate distorted breast datasets from the original human subject data. Segmented bCT data from two different human subjects were used as the 'base' and 'target' for morphing. Several combinations of transformations were applied to morph between the 'base' and 'target' datasets such as changing the breast shape, rotating the glandular data, and changing the distribution of the glandular tissue. Following the morphing, regions of skin and fat were assigned to the morphed dataset in order to appropriately assign mechanical properties during the compression simulation. The resulting morphed breast was compressed using a finite element algorithm and simulated mammograms were generated using techniques described previously. Sixty-two simulated mammograms, generated from morphing three human subject datasets, were used in a preliminary observer evaluation where four board certified breast radiologists with varying amounts of experience ranked the level of realism (from 1 ='fake' to 10 ='real') of the simulated images. Results: The morphing technique was able to successfully generate new and unique morphed datasets from the original human subject data. The radiologists evaluated the realism of simulated mammograms generated from the morphed and unmorphed human subject datasets and scored the realism with an average ranking of 5.87 {+-} 1.99, confirming that overall the phantom image datasets appeared more 'real' than 'fake.' Moreover, there was not a significant difference (p > 0.1) between the realism of the unmorphed datasets (6.0 {+-} 1.95) compared to the morphed datasets (5.86 {+-} 1.99). Three of the four observers had overall average rankings of 6.89 {+-} 0.89, 6.9 {+-} 1.24, 6.76 {+-} 1.22, whereas the fourth observer ranked them noticeably lower at 2.94 {+-} 0.7. Conclusions: This work presents a technique that can be used to generate a suite of realistic computerized breast phantoms from a limited number of human subjects. This suite of flexible breast phantoms can be used for multimodality imaging research to provide a known truth while concurrently producing realistic simulated imaging data.

  20. Quantification of total mercury in liver and heart tissue of Harbor Seals (Phoca vitulina) from Alaska USA

    SciTech Connect (OSTI)

    Marino, Kady B. [Department of Chemistry, Roger Williams University, Bristol, RI 02809 (United States)] [Department of Chemistry, Roger Williams University, Bristol, RI 02809 (United States); Hoover-Miller, Anne; Conlon, Suzanne; Prewitt, Jill [Alaska SeaLife Center, City of Seward, AK (United States)] [Alaska SeaLife Center, City of Seward, AK (United States); O'Shea, Stephen K., E-mail: soshea@rwu.edu [Department of Chemistry, Roger Williams University, Bristol, RI 02809 (United States)

    2011-11-15

    This study quantified the Hg levels in the liver (n=98) and heart (n=43) tissues of Harbor Seals (Phoca vitulina) (n=102) harvested from Prince William Sound and Kodiak Island Alaska. Mercury tissue dry weight (dw) concentrations in the liver ranged from 1.7 to 393 ppm dw, and in the heart from 0.19 to 4.99 ppm dw. Results of this study indicate liver and heart tissues' Hg ppm dw concentrations significantly increase with age. Male Harbor Seals bioaccumulated Hg in both their liver and heart tissues at a significantly faster rate than females. The liver Hg bioaccumulation rates between the harvest locations Kodiak Island and Prince William Sound were not found to be significantly different. On adsorption Hg is transported throughout the Harbor Seal's body with the partition coefficient higher for the liver than the heart. No significant differences in the bio-distribution (liver:heart Hg ppm dw ratios (n=38)) values were found with respect to either age, sex or geographic harvest location. In this study the age at which Hg liver and heart bioaccumulation levels become significantly distinct in male and female Harbor Seals were identified through a Tukey's analysis. Of notably concern to human health was a male Harbor Seal's liver tissue harvested from Kodiak Island region. Mercury accumulation in this sample tissue was determined through a Q-test to be an outlier, having far higher Hg concentrarion (liver 392 Hg ppm dw) than the general population sampled. - Highlights: Black-Right-Pointing-Pointer Mercury accumulation in the liver and heart of seals exceed food safety guidelines. Black-Right-Pointing-Pointer Accumulation rate is greater in males than females with age. Black-Right-Pointing-Pointer Liver mercury accumulation is greater than in the heart tissues. Black-Right-Pointing-Pointer Mercury determination by USA EPA Method 7473 using thermal decomposition.

  1. Adaptive processing of thin structures to augment segmentation of dual-channel structural MRI of the human brain

    E-Print Network [OSTI]

    Withers, James

    2010-01-01

    This thesis presents a method for the segmentation of dual-channel structural magnetic resonance imaging (MRI) volumes of the human brain into four tissue classes. The state-of-the-art FSL FAST segmentation software ...

  2. Chondrogenesis and mineralization during in vitro culture of human stem cells on 3D-woven scaffolds

    E-Print Network [OSTI]

    Abrahamsson, Christoffer K.

    Human mesenchymal stem cells (hMSCs) and three-dimensional (3D) woven poly(?-caprolactone) (PCL) scaffolds are promising tools for skeletal tissue engineering. We hypothesized that in vitro culture duration and medium ...

  3. Composites structures for bone tissue reconstruction

    SciTech Connect (OSTI)

    Neto, W.; Santos, Joo; Avrous, L.; Schlatter, G.; Bretas, Rosario

    2015-05-22

    The search for new biomaterials in the bone reconstitution field is growing continuously as humane life expectation and bone fractures increase. For this purpose, composite materials with biodegradable polymers and hydroxyapatite (HA) have been used. A composite material formed by a film, nanofibers and HA has been made. Both, the films and the non-woven mats of nanofibers were formed by nanocomposites made of butylene adipate-co-terephthalate (PBAT) and HA. The techniques used to produce the films and nanofibers were spin coating and electrospinning, respectively. The composite production and morphology were evaluated. The composite showed an adequate morphology and fibers size to be used as scaffold for cell growth.

  4. Fiber delivered probe for efficient CARS imaging of tissues

    E-Print Network [OSTI]

    Potma, Eric Olaf

    Fiber delivered probe for efficient CARS imaging of tissues Mihaela Balu1, Gangjun Liu1, Zhongping-Stokes Raman scattering (CARS) signal in biological tissues. We discuss the design challenges including capturing the back- scattered forward generated CARS signal in the sample and the effects of fiber

  5. In vitro multiplication of Cucumis sativus, L. through tissue culture

    E-Print Network [OSTI]

    LeRoy, Thomas Russell

    1977-01-01

    of carrot and Jerusalem artichoke showed a greater relative increase in size than larger tissue pieces (7). Meristem tissue culture has been used in the development of disease-free stock (2). This has been important in many of the plants...

  6. Determining the mechanical properties of equine laminar corium tissue

    E-Print Network [OSTI]

    Hallab, Nadim James

    1991-01-01

    elastic orthotropic material. BACKGROUND Mechanical Testing of Biological Materials The field of biomechanics has long dealt with the issue of mechanically characterizing soft and hard tissue. Biological tissue has eluded well defined characterization... Toby Selcer for his assistance with the MTS testing system. TABLE OF CONTENTS ABSTRACT ACKNOWLEDGEMENTS, TABLE OF CONTENTS. . LIST OF TABLES. . LIST OF FIGURES. . INTRODUCTION. Motivation. Objectives. BACKGROUND. . Mechanical Testing...

  7. Discriminative, Semantic Segmentation of Brain Tissue in MR Images

    E-Print Network [OSTI]

    Soatto, Stefano

    Discriminative, Semantic Segmentation of Brain Tissue in MR Images Zhao Yi1 , Antonio Criminisi2 , Jamie Shotton2 , and Andrew Blake2 1 University of California, Los Angeles, USA. zyi@ucla.edu. 2 is presented for the automatic segmentation and classification of brain tissue from 3D MR scans. It uses

  8. Signal processing in scanning thermoacoustic tomography in biological tissues

    E-Print Network [OSTI]

    Wang, Lihong

    Signal processing in scanning thermoacoustic tomography in biological tissues Yuan Xu and Lihong V Microwave-induced thermoacoustic tomography was explored to image biological tissues. Short microwave pulses-induced thermoacoustic waves were detected with a focused ultrasonic transducer to obtain two-dimensional tomographic

  9. Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away

    E-Print Network [OSTI]

    Brenner, David Jonathan

    in extrapolating radiation risk estimates from epidemi- ologically accessible doses down to very low doses where) and for assessing the risk from a low-dose exposure to a carcinogen such as ionizing radiation, where only a small, New York, NY 10032; Radiation Biology Laboratory, Research and Environmental Surveillance, Radiation

  10. Aging effects on DNA methylation modules in human brain and blood tissue

    E-Print Network [OSTI]

    2012-01-01

    error Total variation Proportion of total F variancecan determine what proportion of the variation in eigengene-of variation Source ave.kME.green, total proportion of

  11. Modeling of Human Brain Tissues and Head Injuries Induced by Blast and Ballistic Impact

    E-Print Network [OSTI]

    Kulkarni, Sahil G

    2013-11-07

    .............................................................................. 162 56 Absence of the fluid cavitation on the coup side in the CSF for a helmet protected head ........................................................................ 163 57 Fluid cavitation damage observed on the coup side in the CSF... to the impact site. Irreversible cavitation damage was also observed. However...

  12. Tissue Engineering the TMJ Condyle using Human Umbilical Cord Mesenchymal Stromal Cells

    E-Print Network [OSTI]

    Wang, Limin

    2008-09-09

    emerging scaffold fabrication techniques, including electrospinning, 6 rapid prototyping, 7 fused deposition modeling (FDM), 8 and other solid free-form (SFF) methods, 9-11 have been applied to fabricate complex osteochondral scaffolds...

  13. Beyond differential expression : methods and tools for mining the transcriptomic landscape of human tissue and disease

    E-Print Network [OSTI]

    Schmid, Patrick R. (Patrick Raphael)

    2012-01-01

    Although there are a variety of high-throughput technologies used to perform biological experiments, DNA microarrays have become a standard tool in the modern biologist's arsenal. Microarray experiments provide measurements ...

  14. Brown Adipose Tissue Quantification in Human Neonates Using Water-Fat Separated MRI

    E-Print Network [OSTI]

    2013-01-01

    Nguyen 2 , Theo G. M. van Erp 1 , Ana Guijarro 3 , FaribaJM, Entringer S, Nguyen A, van Erp TGM, Guijarro A, et al. (

  15. Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues

    E-Print Network [OSTI]

    2010-01-01

    Standards Institute (ANSI)-accredited standards developmentBARALLON ET AL. zation. ANSI accreditation ensures thatSystem (PINS) information to ANSI PINS published in ANSI

  16. Aging effects on DNA methylation modules in human brain and blood tissue

    E-Print Network [OSTI]

    2012-01-01

    changes in chromosomes cause aging? Cell 1996, 86:9-12. 2.M: Cross-Talk between Aging and Cancer. Annals of the NewMF, Esteller M: Epigenetics and aging: the targets and the

  17. VISTA Enhancer Browser--A Database of Tissue-Specific Human Enhancers

    E-Print Network [OSTI]

    Visel, Axel; Minovitsky, Simon; Dubchak, Inna; Pennacchio, Len A.

    2006-01-01

    html-template.sourceforge.net) and overLIB (http://www.bosrup.com/web/web interface was developed in Perl using ImageMagick (http://www.imagemagick.org), HTML::

  18. Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths

    E-Print Network [OSTI]

    signal intensities. #12;iv DEDICATION This thesis is dedicated to the people I love. #12;v.............................................................................................................................. ii Dedication

  19. Genome Wide Evaluation of Normal Human Tissue in Response to Controlled, In

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journalvivo Low-Dose Low LET Ionizing Radiation Exposure: Pathways and

  20. Regeneration of Tissues and Organs Using Autologous Cells

    SciTech Connect (OSTI)

    Anthony Atala

    2010-04-28

    The Joint Commission for Health Care Organizations recently declared the shortage of transplantable organs and tissues a public health crisis. As such, there is about one death every 30 seconds due to organ failure. Complications and rejection are still significant albeit underappreciated problems. It is often overlooked that organ transplantation results in the patient being placed on an immune suppression regimen that will ultimate shorten their life span. Patients facing reconstruction often find that surgery is difficult or impossible due to the shortage of healthy autologous tissue. In many cases, autografting is a compromise between the condition and the cure that can result in substantial diminution of quality of life. The national cost of caring for persons who might benefit from engineered tissues or organs has reached $600 billion annually. Autologous tissue technologies have been developed as an alternative to transplantation or reconstructive surgery. Autologous tissues derived from the patient's own cells are capable of correcting numerous pathologies and injuries. The use of autologous cells eliminates the risks of rejection and immunological reactions, drastically reduces the time that patients must wait for lifesaving surgery, and negates the need for autologous tissue harvest, thereby eliminating the associated morbidities. In fact, the use of autologous tissues to create functional organs is one of the most important and groundbreaking steps ever taken in medicine. Although the basic premise of creating tissues in the laboratory has progressed dramatically, only a limited number of tissue developments have reached the patients to date. This is due, in part, to the several major technological challenges that require solutions. To that end, we have been in pursuit of more efficient ways to expand cells in vitro, methods to improve vascular support so that relevant volumes of engineered tissues can be grown, and constructs that can mimic the native tissue environment to ensure tissue integration, maturation, and survival. Other long-term benefits of this research are likely to be cell-based drug delivery mechanisms, intelligent biomaterials, bio-nano technologies, as well as controlled delivery using advances in materials science. The major challenges to the goal of producing tissues and organs for transplantation and reconstruction are three-fold and will form the basis for the goals of this research. These include (1) Identifying sources of autologous cells and developing methods to expand them in large number in vitro, (2) Providing vascular support for growing constructs, and (3) Developing biomaterials and bioreactor systems that mimic the native tissue environment.

  1. Tissue-based standoff biosensors for detecting chemical warfare agents

    DOE Patents [OSTI]

    Greenbaum, Elias; Sanders, Charlene A.

    2003-11-18

    A tissue-based, deployable, standoff air quality sensor for detecting the presence of at least one chemical or biological warfare agent, includes: a cell containing entrapped photosynthetic tissue, the cell adapted for analyzing photosynthetic activity of the entrapped photosynthetic tissue; means for introducing an air sample into the cell and contacting the air sample with the entrapped photosynthetic tissue; a fluorometer in operable relationship with the cell for measuring photosynthetic activity of the entrapped photosynthetic tissue; and transmitting means for transmitting analytical data generated by the fluorometer relating to the presence of at least one chemical or biological warfare agent in the air sample, the sensor adapted for deployment into a selected area.

  2. Repressor-mediated tissue-specific gene expression in plants

    DOE Patents [OSTI]

    Meagher, Richard B. (Athens, GA); Balish, Rebecca S. (Oxford, OH); Tehryung, Kim (Athens, GA); McKinney, Elizabeth C. (Athens, GA)

    2009-02-17

    Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.

  3. P4.37 INTER-COMPARISON OF GOES-8 IMAGER AND SOUNDER SKIN TEMPERATURE RETRIEVALS

    E-Print Network [OSTI]

    Haines, Stephanie L.

    * University of Alabama in Huntsville Global Hydrology and Climate Center, Huntsville, AL Ronnie J. Suggs and Gary J. Jedlovec Global Hydrology and Climate Center MSFC/NASA, Huntsville, AL 1. INTRODUCTION Skin (GOES) data at the Global Hydrology and Climate Center (GHCC). The GOES Imager or Sounder data are used

  4. Characterization of Urinary Iron Loss in the fsn (flaky skin) Anemia Mouse Mutant

    E-Print Network [OSTI]

    Kress, Robert Lee

    2014-08-31

    target to treat iron overload. The flakyskin anemia (fsn) mouse possesses a mutation in the Ttc7 gene (tetratricopeptide repeat domain 7) and had been observed to excrete elevated iron levels in its urine. We hypothesized that the mutation in fsn...

  5. ON THE INFLUENCE OF THE GEOMETRY ON SKIN EFFECT IN ELECTROMAGNETISM

    E-Print Network [OSTI]

    Faou, Erwan

    ON THE INFLUENCE OF THE GEOMETRY ON SKIN EFFECT IN ELECTROMAGNETISM GABRIEL CALOZ, MONIQUE DAUGE, ERWAN FAOU, VICTOR PERON ABSTRACT. We consider the equations of electromagnetism set on a domain made in electromagnetism. This effect describes the rapid decay of electromagnetic fields with depth inside a metallic

  6. Isolation of stem cells from adult telogen skin Elizabeth Deschene Greco Lab

    E-Print Network [OSTI]

    Greco, Valentina

    in the CO2 chamber If you are using a transgenic fluorescent mice, verify at the dissection microscope it with a 25 ml plastic pipette for about 1 minute per skin. Filter the trypsin fraction first with 40 micron(-) serum (chelexed serum) on a shaker. When you have filtered the trypsin fraction you can filter this 10

  7. Vessel Segmentation and Analysis in Laboratory Skin Transplant Micro-angiograms

    E-Print Network [OSTI]

    Lbeck, Universitt zu

    Vessel Segmentation and Analysis in Laboratory Skin Transplant Micro-angiograms Alexandru transplantations depends on the adequate revascularization of the trans- planted dermal matrix. To induce vessel and length of newly grown vessels have to be measured in micro-angiograms (x-ray images of the blood vessels

  8. Water skin anomalies: density, elasticity, hydrophobicity, thermal stability, interface repulsivity, etc

    E-Print Network [OSTI]

    Chang Q. Sun

    2015-02-26

    Molecular undercoordination induced O:H-O bond relaxation and dual polarization dictates the supersolid behavior of water skins interacting with other substances such as flowing in nanochannels, dancing of water droplets, floating of insects. The BOLS-NEP notion unifies the Wenzel-Cassie-Baxter models and explains controllable transition between hydrophobicity and hydrophilicity.

  9. Experimental evaluation of a naturally ventilated PV double-skin building envelope in real operating conditions

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    . Introduction France is undergoing an energy transition towards technologies with a lower environmental impact Fax. +33472438811 Abstract Building integrated photovoltaic systems are fast becoming a feature of a prototype naturally-ventilated photovoltaic double-skin facade, designed to maintain favourable operating

  10. Hydraulics of a finite-diameter horizontal well with wellbore storage and skin effect

    E-Print Network [OSTI]

    Zhan, Hongbin

    Hydraulics of a finite-diameter horizontal well with wellbore storage and skin effect Eungyu Park, hydrogeologists have studied hydraulics of hori- zontal wells in shallow ground water aquifers [5,43,50 52 from the aquifer. Extensive studies on hydraulics of finite or large di- ameter vertical wells

  11. Localization of Fiducial Skin Markers in MR Images using Correlation Pattern

    E-Print Network [OSTI]

    , as is the case with MRI and PET imagery. The process of automating the detection of these markers needs moreLocalization of Fiducial Skin Markers in MR Images using Correlation Pattern Recognition for PET , Roger Easton1 1 Chester F. Carlson Center for Imaging Science at the Rochester Institute of Technology

  12. Density slope of the nuclear symmetry energy from the neutron skin thickness of heavy nuclei

    E-Print Network [OSTI]

    Chen, Lie-Wen; Ko, Che Ming; Li, Bao-An; Xu, Jun.

    2010-01-01

    of finite nuclei and nuclear matter properties. We find that existing data on neutron skin thickness Delta r(np) of Sn isotopes give an important constraint on the symmetry energy E(sym)(rho(0)) and its density slope L at saturation density rho(0). Combining...

  13. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  14. KRFTWRK Global Human Electricity

    E-Print Network [OSTI]

    Prohaska, Rainer

    2009-01-01

    in the generation of human power, where energy is gained byparticipant runs a virtual human power plant. Per every "by muscle-power and chemical processes of human bodies. For

  15. Human-machine interactions

    DOE Patents [OSTI]

    Forsythe, J. Chris (Sandia Park, NM); Xavier, Patrick G. (Albuquerque, NM); Abbott, Robert G. (Albuquerque, NM); Brannon, Nathan G. (Albuquerque, NM); Bernard, Michael L. (Tijeras, NM); Speed, Ann E. (Albuquerque, NM)

    2009-04-28

    Digital technology utilizing a cognitive model based on human naturalistic decision-making processes, including pattern recognition and episodic memory, can reduce the dependency of human-machine interactions on the abilities of a human user and can enable a machine to more closely emulate human-like responses. Such a cognitive model can enable digital technology to use cognitive capacities fundamental to human-like communication and cooperation to interact with humans.

  16. DIVISION OF HUMAN RESOURCES

    E-Print Network [OSTI]

    Salama, Khaled

    you sneeze or cough; cough or sneeze into a tissue and dispose of used tissues. Wash your hands after sneezing or coughing. Avoid touching your eyes, nose or mouth. Avoid others with respiratory illnesses/or cough stuffy nose and /or chills headache, body aches, and /or fatigue Some people with H1N1 flu also

  17. Microfluidics for Tissue and Cell Applications JST CREST, "Cell and Tissue Showcasing by Micro-Nano Integrated Devices" Project

    E-Print Network [OSTI]

    Tokyo, University of

    Microfluidics for Tissue and Cell Applications JST CREST, "Cell and Tissue Showcasing by Micro-Nano Integrated Devices" Project JST-VINNOVA/SSF SICP, "Microfluidic Cancer Diagnosis Platform" Project JST ERATO 2 : Prof. Shuichi Takayama (University of Michigan) "Microfluidic Tools to Model and Analyze

  18. Pulse testing in the presence of wellbore storage and skin effects

    SciTech Connect (OSTI)

    Ogbe, D.O.; Brigham, W.E.

    1984-08-01

    A pulse test is conducted by creating a series of short-time pressure transients in an active (pulsing) well and recording the observed pressure response at an observation (responding) well. Using the pressure response and flow rate data, the transmissivity and storativity of the tested formation can be determined. Like any other pressure transient data, the pulse-test response is significantly influenced by wellbore storage and skin effects. The purpose of this research is to examine the influence of wellbore storage and skin effects on interference testing in general and on pulse-testing in particular, and to present the type curves and procedures for designing and analyzing pulse-test data when wellbore storage and skin effects are active at either the responding well or the pulsing well. A mathematical model for interference testing was developed by solving the diffusivity equation for radial flow of a single-phase, slightly compressible fluid in an infinitely large, homogeneous reservoir. When wellbore storage and skin effects are present in a pulse test, the observed response amplitude is attenuated and the time lag is inflated. Consequently, neglecting wellbore storage and skin effects in a pulse test causes the calculated storativity to be over-estimated and the transmissivity to be under-estimated. The error can be as high as 30%. New correlations and procedures are developed for correcting the pulse response amplitude and time lag for wellbore storage effects. Using these correlations, it is possible to correct the wellbore storage-dominated response amplitude and time lag to within 3% of their expected values without wellbore storage, and in turn to calculate the corresponding transmissivity and storativity. Worked examples are presented to illustrate how to use the new correction techniques. 45 references.

  19. Jefferson Lab Human Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Resources The Human Resources team is fully integrated with Jefferson Lab's mission, committed to providing quality customer service based on expertise, innovation and...

  20. Regeneration of Tissues and Organs Using Autologous Cells

    SciTech Connect (OSTI)

    Anthony Atala, M.D.

    2012-10-11

    The proposed work aims to address three major challenges to the field of regenerative medicine: 1) the growth and expansion of regenerative cells outside the body in controlled in vitro environments, 2) supportive vascular supply for large tissue engineered constructs, and 3) interactive biomaterials that can orchestrate tissue development in vivo. Toward this goal, we have engaged a team of scientists with expertise in cell and molecular biology, physiology, biomaterials, controlled release, nanomaterials, tissue engineering, bioengineering, and clinical medicine to address all three challenges. This combination of resources, combined with the vast infrastructure of the WFIRM, have brought to bear on projects to discover and test new sources of autologous cells that can be used therapeutically, novel methods to improve vascular support for engineered tissues in vivo, and to develop intelligent biomaterials and bioreactor systems that interact favorably with stem and progenitor cells to drive tissue maturation. The Institute??s ongoing programs are aimed at developing regenerative medicine technologies that employ a patient??s own cells to help restore or replace tissue and organ function. This DOE program has provided a means to solve some of the vexing problems that are germane to many tissue engineering applications, regardless of tissue type or target disease. By providing new methods that are the underpinning of tissue engineering, this program facilitated advances that can be applied to conditions including heart disease, diabetes, renal failure, nerve damage, vascular disease, and cancer, to name a few. These types of conditions affect millions of Americans at a cost of more than $400 billion annually. Regenerative medicine holds the promise of harnessing the body??s own power to heal itself. By addressing the fundamental challenges of this field in a comprehensive and focused fashion, this DOE program has opened new opportunities to treat conditions where other approaches have failed.

  1. Characterization of Lung Tissues using Liquid-Crystal Tunable Filter and Hyperspectral Imaging System

    E-Print Network [OSTI]

    Won, Chang-Hee

    Characterization of Lung Tissues using Liquid-Crystal Tunable Filter and Hyperspectral Imaging to characterize lung tissue for detecting emphysematous tissues in lung volume reduction surgery. The system, the spectral signature of healthy lung tissue and simulated smokers lung tissue is obtained and compared

  2. human spaceflight and operations

    E-Print Network [OSTI]

    Anand, Mahesh

    1 human spaceflight and operations Lunar Lander human spaceflight and operations #12;2 human cooperation in exploration prim ary objective opportunity for investigations #12;3 human spaceflight No RHUs LANDING SITE South Polar Reliant on Solar Power generation + conventional thermal control LAUNCHER

  3. Energy-dependence of skin-mode fraction in $E1$ excitations of neutron-rich nuclei

    E-Print Network [OSTI]

    H. Nakada; T. Inakura; H. Sawai

    2015-02-17

    We have extensively investigated characters of the low-energy $E1$ strengths in $N>Z$ nuclei, by analyzing the transition densities obtained by the HF+RPA calculations with several effective interactions. Crossover behavior has been confirmed, from the skin mode at low energy to the $pn$ mode at higher energy. Decomposing the $E1$ strengths into the skin-mode, $pn$-mode and interference fractions, we show that the ratio of the skin-mode strength to the full strength may be regarded as a generic function of the excitation energy, insensitive to nuclides and effective interactions, particularly beyond Ni.

  4. Modulating tissue mechanics to increase oxygen delivery to tumors

    E-Print Network [OSTI]

    Martin, John Daniel, Ph. D. Massachusetts Institute of Technology

    2015-01-01

    Solid tumors have low oxygen tension - hypoxia - that fuels disease progression and treatment resistance. Thus, strategies for alleviating hypoxia are needed. Two factors affect tissue oxygen levels: oxygen supply via blood ...

  5. New Methods in Tissue Engineering: Improved Models for Viral Infection

    E-Print Network [OSTI]

    Ramanan, Vyas

    New insights in the study of virus and host biology in the context of viral infection are made possible by the development of model systems that faithfully recapitulate the in vivo viral life cycle. Standard tissue culture ...

  6. Tissue-specific gene silencing monitored in circulating RNA

    E-Print Network [OSTI]

    Sehgal, Alfica

    Pharmacologic target gene modulation is the primary objective for RNA antagonist strategies and gene therapy. Here we show that mRNAs encoding tissue-specific gene transcripts can be detected in biological fluids and that ...

  7. Injectable hyaluronic acid scaffolds for cartilage tissue engineering

    E-Print Network [OSTI]

    Ren, Cindy D

    2008-01-01

    Every year tens of millions worldwide suffer from cartilage damage, caused by mechanical degradation, trauma or disease. Because of the lack of blood supply and low cell concentration within the tissue, cartilage has very ...

  8. Third- and fourth-order elasticity of biological soft tissues

    E-Print Network [OSTI]

    Michel Destrade; Michael D. Gilchrist; Raymond W. Ogden

    2013-01-24

    In the theory of weakly non-linear elasticity, Hamilton et al. [J. Acoust. Soc. Am. \\textbf{116} (2004) 41] identified $W = \\mu I_2 + (A/3)I_3 + D I_2^2$ as the fourth-order expansion of the strain-energy density for incompressible isotropic solids. Subsequently, much effort focused on theoretical and experimental developments linked to this expression in order to inform the modeling of gels and soft biological tissues. However, while many soft tissues can be treated as incompressible, they are not in general isotropic, and their anisotropy is associated with the presence of oriented collagen fiber bundles. Here the expansion of $W$ is carried up to fourth-order in the case where there exists one family of parallel fibers in the tissue. The results are then applied to acoustoelasticity, with a view to determining the second- and third-order nonlinear constants by employing small-amplitude transverse waves propagating in a deformed soft tissue.

  9. Study of Cell Material Interactions for Vascular Tissue Engineering Application

    E-Print Network [OSTI]

    Qu, Xin

    2012-07-16

    . Combined, our results suggested interesting roles for fibronectin and HA_IMW in repression of undesired phenotypes in SMC differentiation, which could give insights into rational design of novel biomaterials for vascular tissue engineering applications....

  10. Distribution and metabolism of antibodies and macromolecules in tumor tissue

    E-Print Network [OSTI]

    Thurber, Greg M

    2008-01-01

    Tumor targeting drugs that selectively treat cancerous tissue are promising agents for lowering the morbidity and mortality of cancer. Within this field, antibody treatments for cancer are currently being developed for ...

  11. Culture of cells from mammalian tissue cryopreserved without cryoprotection

    E-Print Network [OSTI]

    Charles, Lara Nicole

    2009-05-15

    Donor cells for nuclear transfer are usually prepared by the culture of fresh tissue. However, animal carcasses are sometimes frozen without cryoprotectants and if it were possible to obtain live cells from carcasses ...

  12. Collagen scaffolds and injectable biopolymer gels for cardiac tissue engineering

    E-Print Network [OSTI]

    Ng, Karen Kailin

    2012-01-01

    Three-dimensional biomaterial scaffolds have begun to shown promise for cell delivery for cardiac tissue engineering. Although various polymers and material forms have been explored, there is a need for: injectable gels ...

  13. Development of novel dynamic indentation techniques for soft tissue applications

    E-Print Network [OSTI]

    Balakrishnan, Asha, 1974-

    2007-01-01

    Realistic material models to simulate the behavior of brain tissue at large deformations and high strain rates are necessary when designing equipment to protect against ballistic impacts. Acquiring experimental data for ...

  14. Adipogenesis and angiogenesis : roles in tissue engineering and glucose metabolism

    E-Print Network [OSTI]

    Tam, Joshua

    2009-01-01

    Adipose tissue serves two main functions in the body: (1) it is the body's primary energy depot; and (2) it also serves as an important endocrine organ, producing and secreting various enzymes, growth factors, cytokines, ...

  15. Increasing the safety and precision of medical tissue puncture

    E-Print Network [OSTI]

    Begg, Nikolai David Michael

    2014-01-01

    Tissue puncture is ubiquitous in medicine, from percutaneous injections and biopsies to laparoscopic surgical access, epidural anesthesia, and cranial drilling; over 10 million puncture procedures are performed each year ...

  16. BE.410J Molecular, Cellular and Tissue Biomechanics, Spring 2003

    E-Print Network [OSTI]

    Kamm, Roger D.

    This course develops and applies scaling laws and the methods of continuum mechanics to biomechanical phenomena over a range of length scales. Topics include: structure of tissues and the molecular basis for macroscopic ...

  17. Intrinsic optical signals in neural tissues: measurements, mechanisms, and applications

    E-Print Network [OSTI]

    Fang-Yen, Christopher

    Intrinsic optical signals in neural tissues: measurements, mechanisms, and applications Christopher optical signals, including changes in absorption, scattering, birefringence, refractive index, and nerve in scattering, birefringence (2), and optical activity (3) during the action potential in numerous invertebrate

  18. Developing osteoarthritis treatments through cartilage tissue engineering and molecular imaging

    E-Print Network [OSTI]

    Casasnovas Ortega, Nicole

    2012-01-01

    Tissue engineering can be applied to develop therapeutic techniques for osteoarthritis, a degenerative disease caused by the progressive deterioration of cartilage in joints. An inherent goal in developing cartilage-replacement ...

  19. Driving tissue morphogenetic cascades using tunable nanolayered surface coatings

    E-Print Network [OSTI]

    Shah, Nisarg Jaydeep

    2014-01-01

    Harnessing the synergy between materials at the nanoscale can be a valuable tool in understanding and probing cellular phenomena and in driving specific processes that lead to tissue and organ regeneration and repair. ...

  20. Novel polypyrrole derivatives to enhance conductive polymer-tissue interactions

    E-Print Network [OSTI]

    George, Paul M. (Paul Matthew)

    2005-01-01

    Developing materials that interact effectively with surrounding tissue is a major obstacle in sensor and drug delivery research. The body's natural immune response prevents foreign objects from easily integrating with an ...

  1. Fabrication of tissue scaffolds using projection micro-stereolithography

    E-Print Network [OSTI]

    Matsushita, Albert Keisuke

    2015-01-01

    In vitro liver models are a critical tool in pharmaceutical research, yet standard hepatocyte cultures fail to capture the complexity of in vivo tissue behavior. One of the most critical features of the in vivo liver is ...

  2. Design, construction and implementation of spherical tissue equivalent proportional counter

    E-Print Network [OSTI]

    Perez Nunez, Delia Josefina

    2009-05-15

    and the response to neutrons as a function of angle was constant 7%. This spherical tissue equivalent proportional counter detector system will improve the accuracy of dosimetry in space, and as a result improve radiation safety for astronauts....

  3. Polyelectrolyte multilayer growth factor delivery : mediating tissue/device interactions

    E-Print Network [OSTI]

    Macdonald, Mara Lee

    2010-01-01

    This thesis focuses on the use of ultrathin therapeutic protein delivery films to control host tissue/medical device implant interactions, thereby reducing complications that lead to implant failure. The Layer by Layer ...

  4. BE.441 Biomaterials-Tissue Interactions, Fall 2003

    E-Print Network [OSTI]

    Spector, Myron

    Principles of materials science and cell biology underlying the design of medical implants, artificial organs, and matrices for tissue engineering. Methods for biomaterials surface characterization and analysis of protein ...

  5. Development and Testing of a Portable Multi-Channel Depth-Resolved Near Infrared Spectroscopy System for Lower Leg Tissue Oxygenation Monitoring

    E-Print Network [OSTI]

    Kostic, Marko N.

    2013-01-01

    Layers of the Skin, SEER Training Modules. U. S. Nationalthe Skin, SEER Training Modules. U. S. National Institutesof Skeletal Muscle, SEER Training Modules. U. S. National

  6. Heat transfers in a double-skin roof ventilated by natural convection in summer time

    E-Print Network [OSTI]

    Biwole, Pascal; Pompeo, C

    2013-01-01

    The double-skin roofs investigated in this paper are formed by adding a metallic screen on an existing sheet metal roof. The system enhances passive cooling of dwellings and can help diminishing power costs for air conditioning in summer or in tropical and arid countries. In this work, radiation, convection and conduction heat transfers are investigated. Depending on its surface properties, the screen reflects a large amount of oncoming solar radiation. Natural convection in the channel underneath drives off the residual heat. The bi-dimensional numerical simulation of the heat transfers through the double skin reveals the most important parameters for the system's efficiency. They are, by order of importance, the sheet metal surface emissivity, the screen internal and external surface emissivity, the insulation thickness and the inclination angle for a channel width over 6 cm. The influence of those parameters on Rayleigh and Nusselt numbers is also investigated. Temperature and air velocity profiles on seve...

  7. Digital Microfluidic Lab-on-a-Chip Platform for Tissue Engineering

    E-Print Network [OSTI]

    Bender, Brian Francis

    2015-01-01

    microfluidics for tissue engineering: state of the art andDifferentiation. Tissue Engineering Part A, 2009. 15(2): p.T. Eschenhagen, Cardiac Tissue Engineering State of the Art.

  8. Development of a high throughput 3D perfused liver tissue bioreactor

    E-Print Network [OSTI]

    Inman, Samuel Walker

    2006-01-01

    This thesis describes the development of a device designed for culturing liver tissue in a 3D perfused environment. Cells form tissue inside miniature channels of a scaffold, and the tissue is perfused with culture medium ...

  9. Volumetric reconstruction of tissue structure from two-dimensional microscopy images

    E-Print Network [OSTI]

    Cruz, Francisco (Francisco Ui)

    2006-01-01

    Cell morphology of tissue is naturally three-dimensional. Most current methods for tissue analysis use two dimensional histological images of the tissue samples, restricting the analysis to 2D. Existing approaches do not ...

  10. THERMAL SKIN DAMAGE AND MOBILE PHONE USE Elmountacer Billah Elabbassi(1)

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    in thé phone by thé battery currents and running of thé radiofrequency (RF) electronic circuits measured thé température of thé temporal skin due to GSM 1800 MHz MP radiated power (125 mW). To perforai a substantial part of thé radiated power is absorbed. Many epidemiological investigations of MP users [1, 2

  11. Tumorigenic action of beta, proton, alpha and electron radiation on the rat skin

    SciTech Connect (OSTI)

    Burns, F.J.

    1980-01-01

    Rat skin is utilized as a model system for studying dose and time related aspects of the oncogenic action of ionizing radiation, ultraviolet light and polycyclic aromatic hydrocarbons. Molecular lesions in the DNA of the epidermis, including strand breaks and thymine dimers, are measured and compared to the temporal and dose related aspects of tumor induction. The induction and repair kinetics of molecular lesions are compared to split dose recovery as modified by sensitizers and type of radition of oncogenic damage.

  12. The Contribution of Tissue Level Organization to Genomic Stability Following Low Dose/Low Dose Rate Gamma and Proton Irradiation

    SciTech Connect (OSTI)

    Cheryl G. Burrell, Ph.D.

    2012-05-14

    The formation of functional tissue units is necessary in maintaining homeostasis within living systems, with individual cells contributing to these functional units through their three-dimensional organization with integrin and adhesion proteins to form a complex extra-cellular matrix (ECM). This is of particular importance in those tissues susceptible to radiation-induced tumor formation, such as epithelial glands. The assembly of epithelial cells of the thyroid is critical to their normal receipt of, and response to, incoming signals. Traditional tissue culture and live animals present significant challenges to radiation exposure and continuous sampling, however, the production of bioreactor-engineered tissues aims to bridge this gap by improve capabilities in continuous sampling from the same functional tissue, thereby increasing the ability to extrapolate changes induced by radiation to animals and humans in vivo. Our study proposes that the level of tissue organization will affect the induction and persistence of low dose radiation-induced genomic instability. Rat thyroid cells, grown in vitro as 3D tissue analogs in bioreactors and as 2D flask grown cultures were exposed to acute low dose (1, 5, 10 and 200 cGy) gamma rays. To assess immediate (6 hours) and delayed (up to 30 days) responses post-irradiation, various biological endpoints were studied including cytogenetic analyses, apoptosis analysis and cell viability/cytotoxicity analyses. Data assessing caspase 3/7 activity levels show that, this activity varies with time post radiation and that, overall, 3D cultures display more genomic instability (as shown by the lower levels of apoptosis over time) when compared to the 2D cultures. Variation in cell viability levels were only observed at the intermediate and late time points post radiation. Extensive analysis of chromosomal aberrations will give further insight on the whether the level of tissue organization influences genomic instability patterns after low dose radiation exposure. Cells viability/cytotoxicity analysis data are currently being analyzed to determine how these endpoints are affected under our experimental conditions. The results from this study will be translatable to risk assessment for assigning limits to radiation workers, pre-dosing for more effective radiotherapy and the consequences of long duration space flight. The data from this study has been presented a various scientific meetings/workshops and a manuscript, containing the findings, is currently being prepared for publication. Due to unforeseen challenges in collecting the data and standardizing experimental procedures, the second and third aims have not been completed. However, attempts will be made, based on the availability of funds, to continue this project so that these aims can be satisfied.

  13. Investigation of Skin Tribology and Its Effects on Coefficient of Friction and Other Tactile Attributes Involving Polymer Applications

    E-Print Network [OSTI]

    Darden, Matthew Aguirre

    2012-02-14

    OF SKIN TRIBOLOGY AND ITS EFFECTS ON COEFFICIENT OF FRICTION AND OTHER TACTILE ATTRIBUTES INVOLVING POLYMER APPLICATIONS A Thesis by MATTHEW AGUIRRE DARDEN Submitted to the Office of Graduate Studies of Texas A&M University in partial... Tactile Attributes Involving Polymer Applications Copyright 2010 Matthew Aguirre Darden INVESTIGATION OF SKIN TRIBOLOGY AND ITS EFFECTS ON COEFFICIENT OF FRICTION AND OTHER TACTILE ATTRIBUTES INVOLVING POLYMER APPLICATIONS A Thesis...

  14. Development of computational and experimental tools to study mechanotransduction in C.elegans and primates

    E-Print Network [OSTI]

    Kumar, Siddarth

    2011-01-01

    When an object comes into contact with the human fingertip, surface loads imposed on the fingerpad are transmitted to thousands of specialized nerve endings embedded in the skin tissue. These nerve endings, called ...

  15. The effect of head size/shape, miscentering, and bowtie filter on peak patient tissue doses from modern brain perfusion 256-slice CT: How can we minimize the risk for deterministic effects?

    SciTech Connect (OSTI)

    Perisinakis, Kostas; Seimenis, Ioannis; Tzedakis, Antonis; Papadakis, Antonios E.; Damilakis, John

    2013-01-15

    Purpose: To determine patient-specific absorbed peak doses to skin, eye lens, brain parenchyma, and cranial red bone marrow (RBM) of adult individuals subjected to low-dose brain perfusion CT studies on a 256-slice CT scanner, and investigate the effect of patient head size/shape, head position during the examination and bowtie filter used on peak tissue doses. Methods: The peak doses to eye lens, skin, brain, and RBM were measured in 106 individual-specific adult head phantoms subjected to the standard low-dose brain perfusion CT on a 256-slice CT scanner using a novel Monte Carlo simulation software dedicated for patient CT dosimetry. Peak tissue doses were compared to corresponding thresholds for induction of cataract, erythema, cerebrovascular disease, and depression of hematopoiesis, respectively. The effects of patient head size/shape, head position during acquisition and bowtie filter used on resulting peak patient tissue doses were investigated. The effect of eye-lens position in the scanned head region was also investigated. The effect of miscentering and use of narrow bowtie filter on image quality was assessed. Results: The mean peak doses to eye lens, skin, brain, and RBM were found to be 124, 120, 95, and 163 mGy, respectively. The effect of patient head size and shape on peak tissue doses was found to be minimal since maximum differences were less than 7%. Patient head miscentering and bowtie filter selection were found to have a considerable effect on peak tissue doses. The peak eye-lens dose saving achieved by elevating head by 4 cm with respect to isocenter and using a narrow wedge filter was found to approach 50%. When the eye lies outside of the primarily irradiated head region, the dose to eye lens was found to drop to less than 20% of the corresponding dose measured when the eye lens was located in the middle of the x-ray beam. Positioning head phantom off-isocenter by 4 cm and employing a narrow wedge filter results in a moderate reduction of signal-to-noise ratio mainly to the peripheral region of the phantom. Conclusions: Despite typical peak doses to skin, eye lens, brain, and RBM from the standard low-dose brain perfusion 256-slice CT protocol are well below the corresponding thresholds for the induction of erythema, cataract, cerebrovascular disease, and depression of hematopoiesis, respectively, every effort should be made toward optimization of the procedure and minimization of dose received by these tissues. The current study provides evidence that the use of the narrower bowtie filter available may considerably reduce peak absorbed dose to all above radiosensitive tissues with minimal deterioration in image quality. Considerable reduction in peak eye-lens dose may also be achieved by positioning patient head center a few centimeters above isocenter during the exposure.

  16. Programming with human computation

    E-Print Network [OSTI]

    Little, Greg (Danny Greg)

    2011-01-01

    Amazon's Mechanical Turk provides a programmatically accessible micro-task market, allowing a program to hire human workers. This has opened the door to a rich field of research in human computation where programs orchestrate ...

  17. Patenting Human Evolution

    E-Print Network [OSTI]

    Torrance, Andrew W.

    2008-06-01

    to thorough analysis and debate prior to the imminent arrival of human genetic enhancement technologies. Otherwise, patent law may drive human evolution in directions either unplanned - or worse - undesired....

  18. Final LDRD report : development of sample preparation methods for ChIPMA-based imaging mass spectrometry of tissue samples.

    SciTech Connect (OSTI)

    Maharrey, Sean P.; Highley, Aaron M.; Behrens, Richard, Jr.; Wiese-Smith, Deneille

    2007-12-01

    The objective of this short-term LDRD project was to acquire the tools needed to use our chemical imaging precision mass analyzer (ChIPMA) instrument to analyze tissue samples. This effort was an outgrowth of discussions with oncologists on the need to find the cellular origin of signals in mass spectra of serum samples, which provide biomarkers for ovarian cancer. The ultimate goal would be to collect chemical images of biopsy samples allowing the chemical images of diseased and nondiseased sections of a sample to be compared. The equipment needed to prepare tissue samples have been acquired and built. This equipment includes an cyro-ultramicrotome for preparing thin sections of samples and a coating unit. The coating unit uses an electrospray system to deposit small droplets of a UV-photo absorbing compound on the surface of the tissue samples. Both units are operational. The tissue sample must be coated with the organic compound to enable matrix assisted laser desorption/ionization (MALDI) and matrix enhanced secondary ion mass spectrometry (ME-SIMS) measurements with the ChIPMA instrument Initial plans to test the sample preparation using human tissue samples required development of administrative procedures beyond the scope of this LDRD. Hence, it was decided to make two types of measurements: (1) Testing the spatial resolution of ME-SIMS by preparing a substrate coated with a mixture of an organic matrix and a bio standard and etching a defined pattern in the coating using a liquid metal ion beam, and (2) preparing and imaging C. elegans worms. Difficulties arose in sectioning the C. elegans for analysis and funds and time to overcome these difficulties were not available in this project. The facilities are now available for preparing biological samples for analysis with the ChIPMA instrument. Some further investment of time and resources in sample preparation should make this a useful tool for chemical imaging applications.

  19. Effects of Wnt3a on proliferation and differentiation of human epidermal stem cells

    SciTech Connect (OSTI)

    Jia Liwei; Zhou Jiaxi; Peng Sha; Li Juxue [State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101 (China); Graduate School of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100039 (China); Cao Yujing [State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101 (China); Duan Enkui [State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101 (China)], E-mail: duane@ioz.ac.cn

    2008-04-11

    Epidermal stem cells maintain development and homeostasis of mammalian epidermis throughout life. However, the molecular mechanisms involved in the proliferation and differentiation of epidermal stem cells are far from clear. In this study, we investigated the effects of Wnt3a and Wnt/{beta}-catenin signaling on proliferation and differentiation of human fetal epidermal stem cells. We found both Wnt3a and active {beta}-catenin, two key members of the Wnt/{beta}-catenin signaling, were expressed in human fetal epidermis and epidermal stem cells. In addition, Wnt3a protein can promote proliferation and inhibit differentiation of epidermal stem cells in vitro culture. Our results suggest that Wnt/{beta}-catenin signaling plays important roles in human fetal skin development and homeostasis, which also provide new insights on the molecular mechanisms of oncogenesis in human epidermis.

  20. Human Functional Brain Imaging

    E-Print Network [OSTI]

    Rambaut, Andrew

    Human Functional Brain Imaging 19902009 September 2011 Portfolio Review #12;2 | Portfolio Review: Human Functional Brain ImagingThe Wellcome Trust is a charity registered in England and Wales, no's role in supporting human functional brain imaging and have informed `our' speculations for the future

  1. Developments in Human Communication

    E-Print Network [OSTI]

    Thompson, Michael

    CMST 1B03 Developments in Human Communication: Part & Present Amanda Etches-Johnson Mills Research in Human Communication Session Outline 1. Finding Books Using MORRIS 2. Finding Journal Articles using: Developments in Human Communication What is MORRIS anyway? MORRIS is McMaster's library catalogue Shows

  2. Sandia Energy - Human Reliability Assessment

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Reliability Assessment Home Stationary Power Nuclear Fuel Cycle Nuclear Energy Safety Technologies Risk and Safety Assessment Human Reliability Assessment Human Reliability...

  3. Human Factors @ UB Fall 2010 Human Factors

    E-Print Network [OSTI]

    Krovi, Venkat

    . Outsourcing aviation maintenance: Hu- man factors implications, specifically for communications. C. Drury, K. Guy, C. Wenner. International Journal of Aviation Psychology, 2010, 20, 124 143. #12;2 Human Factors

  4. Optical spectroscopy for the detection of ischemic tissue injury

    DOE Patents [OSTI]

    Demos, Stavros (Livermore, CA); Fitzgerald, Jason (Sacramento, CA); Troppmann, Christoph (Sacramento, CA); Michalopoulou, Andromachi (Athens, GR)

    2009-09-08

    An optical method and apparatus is utilized to quantify ischemic tissue and/or organ injury. Such a method and apparatus is non-invasive, non-traumatic, portable, and can make measurements in a matter of seconds. Moreover, such a method and apparatus can be realized through optical fiber probes, making it possible to take measurements of target organs deep within a patient's body. Such a technology provides a means of detecting and quantifying tissue injury in its early stages, before it is clinically apparent and before irreversible damage has occurred.

  5. The Effect of Altered Plasma on Tissue Proliferation

    E-Print Network [OSTI]

    Hoffman, Robert Lee

    1913-01-01

    Center for Digital Scholarship. http://kuscholarworks.ku.edu Submitted to the Department of Anatomy of the University of Kansas in partial fulfillment of the requirements for the Degree of Master of Arts ALTE EMWD P L A S M A 1 ^ The Rffect... regard for asepsis and placed in sterile normal salt solution 0 . 7 $ at 38 0 . The tissues were subsequently divided either by teasing or by careful dissection with delicate tissue scissors; into sizes ranging from 0 . 5 tol. m.m. each. It was found...

  6. Energy loss characteristics of heavy ions in nitrogen, carbon dioxide, argon, hydrocarbon gases and tradescantia tissue

    E-Print Network [OSTI]

    Dennis, J A

    1971-01-01

    Energy loss characteristics of heavy ions in nitrogen, carbon dioxide, argon, hydrocarbon gases and tradescantia tissue

  7. Two new complete genome sequences offer insight into host and tissue specificity of plant pathogenic Xanthomonas spp.

    E-Print Network [OSTI]

    2011-01-01

    Complete Genome Sequences Offer Insight into Host and TissueComplete Genome Sequences Offer Insight into Host and Tissue

  8. Characterization of a MOSkin detector for in vivo skin dose measurements during interventional radiology procedures

    SciTech Connect (OSTI)

    Safari, M. J.; Wong, J. H. D.; Ng, K. H.; Jong, W. L.; Cutajar, D. L.; Rosenfeld, A. B.

    2015-05-15

    Purpose: The MOSkin is a MOSFET detector designed especially for skin dose measurements. This detector has been characterized for various factors affecting its response for megavoltage photon beams and has been used for patient dose measurements during radiotherapy procedures. However, the characteristics of this detector in kilovoltage photon beams and low dose ranges have not been studied. The purpose of this study was to characterize the MOSkin detector to determine its suitability for in vivo entrance skin dose measurements during interventional radiology procedures. Methods: The calibration and reproducibility of the MOSkin detector and its dependency on different radiation beam qualities were carried out using RQR standard radiation qualities in free-in-air geometry. Studies of the other characterization parameters, such as the dose linearity and dependency on exposure angle, field size, frame rate, depth-dose, and source-to-surface distance (SSD), were carried out using a solid water phantom under a clinical x-ray unit. Results: The MOSkin detector showed good reproducibility (94%) and dose linearity (99%) for the dose range of 2 to 213 cGy. The sensitivity did not significantly change with the variation of SSD (1%), field size (1%), frame rate (3%), or beam energy (5%). The detector angular dependence was within 5% over 360 and the dose recorded by the MOSkin detector in different depths of a solid water phantom was in good agreement with the Markus parallel plate ionization chamber to within 3%. Conclusions: The MOSkin detector proved to be reliable when exposed to different field sizes, SSDs, depths in solid water, dose rates, frame rates, and radiation incident angles within a clinical x-ray beam. The MOSkin detector with water equivalent depth equal to 0.07 mm is a suitable detector for in vivo skin dosimetry during interventional radiology procedures.

  9. Beagle Dog Tissue Archive (previously part of National Radiobiology Archives): from the Janus Tissue Archive at Northwestern University

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Watson, Charles R.

    Following the advent of the atomic age, many nations have investigated the effects of radioactive exposure in animal models. Some of these investigations involved costly and unique experiments that produced tissue and data archives which are unlikely to be reproduced. In an effort to extract the value from these collections, programs have started in Japan, Europe, and America to preserve and make public the data and tissues from these studies for further investigation. The Beagle Dog Experiments, carried out at Argonne National Laboratory from 1952 to 1991 by Thomas Fritz, William Norris, and Tom Seed and supported by grants from the Atomic Energy Commission, investigated the effects of Cobalt-60 radiation on beagle dogs. Documentation from these studies is availible in pdf form. This web portal seeks to make accessible the animal tissues and study data from the Beagle Dog Experiments using data organized by Charles Watson. Use the search form to the left to look for dog data from particular experimental conditions. Click a dog number to return the full dog record. Use the dog record to find tissues of interest and make a sample tissue request. These tissue samples and the data were known until recently as the the U.S. National Radiobiology Archives (NRA) and were maintained as the United States Transuranium and Uranium Registries (USTUR) at Washington State University. Life-span studies using beagle dogs were done at the Argonne National Laboratory, University of California at Davis, Pacific Northwest National Laboratory, Inhalation Toxicology Research Institute, and the University of Utah. The results and many microscope slides from these life-span studies, totaling some 6000 dogs, are now available to researchers. A seminal work included in the Archive is The Atlas of Experimentally-Induced Neoplasia in the Beagle Dog (Watson et al, 1997).

  10. Mechanical control of tissue growth: Function follows form

    E-Print Network [OSTI]

    Nelson, Celeste M.

    Mechanical control of tissue growth: Function follows form Donald E. Ingber* Harvard Medical School of reciprocal mechanical in- teractions among the material elements that constitute living systems. Many of interest in mechanical forces as morphogenetic reg- ulators. The work of Nelson et al. (1) in this issue

  11. FULL ARTICLE Bond-selective imaging of deep tissue

    E-Print Network [OSTI]

    Cheng, Ji-Xin

    FULL ARTICLE Bond-selective imaging of deep tissue through the optical window between 1600 and 1850 of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA 2 Department of Cellular 2011, accepted 12 November 2011 Published online 29 November 2011 Key words: optical window, deep

  12. Micromolded gelatin hydrogels for extended culture of engineered cardiac tissues

    E-Print Network [OSTI]

    Parker, Kevin Kit

    Micromolded gelatin hydrogels for extended culture of engineered cardiac tissues Megan L. McCain 1 that micromolded gelatin hydrogel substrates tuned to mimic the elastic modulus of the heart would extend gelatin hydrogel muscular thin film cantilevers. Neonatal rat cardiac myocytes adhered to gelatin

  13. A feasibility study of a gelatin-based tissue substitute

    E-Print Network [OSTI]

    Spence, Jody Lee

    1993-01-01

    to reliquify rather easily to allow for recovery of the TLD powder with little or no loss of thermoluminesence. Of the three materials selected for evaluation, a 10% gelatin mixture provided the closest match to Reference Man tissue. The gelatin mixture...

  14. Hybrid Polyethylene Glycol Hydrogels for Tissue Engineering Applications

    E-Print Network [OSTI]

    Munoz Pinto, Dany 1981-

    2012-07-11

    modification of poly(ethylene glycol) hydrogels for tissue engineering? by Junmin Zhu, 2010, Biomaterials 31(17):4639-4656, Copyright 2010, Elsevier Limited. 4 Poly (ethylene glycol) (PEG) hydrogels can be used as a platform in 3D environments in order...

  15. A Web-based Plant Tissue Culture Information System

    E-Print Network [OSTI]

    Herring, April Sky

    2001-01-01

    The World Wide Web recently emerged as a method for scientists to communicate with each other in a more rapid and in a less costly manner. The University of Minnesota hosts the PLANT-TC listserv as a service to the international tissue culture...

  16. Low-coherent backscattering spectroscopy for tissue characterization

    E-Print Network [OSTI]

    Kim, Young L.

    in biological tissue and other random media with long-transport mean-free path lengths, which have been constructive interference of waves trav- eling time-reversed paths; i.e., the first and last points of a path coincide with the last and first points of its time-reversed path, respectively. For a plane wave

  17. Multivariate classification of infrared spectra of cell and tissue samples

    DOE Patents [OSTI]

    Haaland, David M. (Albuquerque, NM); Jones, Howland D. T. (Albuquerque, NM); Thomas, Edward V. (Albuquerque, NM)

    1997-01-01

    Multivariate classification techniques are applied to spectra from cell and tissue samples irradiated with infrared radiation to determine if the samples are normal or abnormal (cancerous). Mid and near infrared radiation can be used for in vivo and in vitro classifications using at least different wavelengths.

  18. Tissue Classification of Noisy MR Brain Images Using Constrained GMM

    E-Print Network [OSTI]

    Goldberger, Jacob

    Tissue Classification of Noisy MR Brain Images Using Constrained GMM Amit Ruf1 , Hayit Greenspan1. The presented algorithm is used to segment 3D, T1weighted, simulated and real MR images of the brain into three , and Jacob Goldberger2 1 Department of Biomedical Engineering, Tel-Aviv University, ISRAEL, 2 School

  19. Probing the neutron-skin thickness by photon production from reactions induced by intermediate-energy protons

    E-Print Network [OSTI]

    Wei, Gao-Feng

    2015-01-01

    Photon from neutron-proton bremsstrahlung in p+Pb reactions is examined as a potential probe of the neutron-skin thickness in different centralities and at different proton incident energies. It is shown that the best choice of reaction environment is about 140MeV for the incident proton and the 95\\%-100\\% centrality for the reaction system since the incident proton mainly interacts with neutrons inside the skin of the target and thus leads to different photon production to maximal extent. Moreover, considering two main uncertainties from both photon production probability and nucleon-nucleon cross section in the reaction, I propose to use the ratio of photon production from two reactions to measure the neutron-skin thickness because of its cancellation effects on these uncertainties simultaneously, but the preserved about 13\\%-15\\% sensitivities on the varied neutron-skin thickness from 0.1 to 0.3fm within the current experimental uncertainty range of the neutron-skin size in $^{208}$Pb.

  20. Mammalian Tissue Response to Low Dose Ionizing Radiation: The Role of Oxidative Metabolism and Intercellular Communication

    SciTech Connect (OSTI)

    Azzam, Edouard I

    2013-01-16

    The objective of the project was to elucidate the mechanisms underlying the biological effects of low dose/low dose rate ionizing radiation in organs/tissues of irradiated mice that differ in their susceptibility to ionizing radiation, and in human cells grown under conditions that mimic the natural in vivo environment. The focus was on the effects of sparsely ionizing cesium-137 gamma rays and the role of oxidative metabolism and intercellular communication in these effects. Four Specific Aims were proposed. The integrated outcome of the experiments performed to investigate these aims has been significant towards developing a scientific basis to more accurately estimate human health risks from exposures to low doses ionizing radiation. By understanding the biochemical and molecular changes induced by low dose radiation, several novel markers associated with mitochondrial functions were identified, which has opened new avenues to investigate metabolic processes that may be affected by such exposure. In particular, a sensitive biomarker that is differentially modulated by low and high dose gamma rays was discovered.

  1. Constraints on neutron skin thickness in 208Pb and density-dependent symmetry energy

    E-Print Network [OSTI]

    Jianmin Dong; Wei Zuo; Jianzhong Gu

    2015-04-09

    Accurate knowledge about the neutron skin thickness $\\Delta R_{np}$ in $^{208}$Pb has far-reaching implications for different communities of nuclear physics and astrophysics. Yet, the novel Lead Radius Experiment (PREX) did not yield stringent constraint on the $\\Delta R_{np}$ recently. We employ a more practicable strategy currently to probe the neutron skin thickness of $^{208}$Pb based on a high linear correlation between the $\\Delta R_{np}$ and $J-a_{\\text{sym}}$, where $J$ and $a_{\\text{sym}}$ are the symmetry energy (coefficient) of nuclear matter at saturation density and of $^{208}$Pb. An accurate $J-a_{\\text{sym}}$ thus places a strong constraint on the $\\Delta R_{np}$. Compared with the parity-violating asymmetry $A_{\\text{PV}}$ in the PREX, the reliably experimental information on the $J-a_{\\text{sym}}$ is much more easily available attributed to a wealth of measured data on nuclear masses and on decay energies. The density dependence of the symmetry energy is also well constrained with the $J-a_{\\text{sym}}$. Finally, with a `tomoscan' method, we find that one just needs to measure the nucleon densities in $^{208}$Pb starting from $R_{m} = 7.61\\pm0.04$ fm to obtain the $\\Delta R_{np}$ in hadron scattering experiments, regardless of its interior profile that is hampered by the strong absorption.

  2. Constraints on neutron skin thickness in 208Pb and density-dependent symmetry energy

    E-Print Network [OSTI]

    Dong, Jianmin; Gu, Jianzhong

    2015-01-01

    Accurate knowledge about the neutron skin thickness $\\Delta R_{np}$ in $^{208}$Pb has far-reaching implications for different communities of nuclear physics and astrophysics. Yet, the novel Lead Radius Experiment (PREX) did not yield stringent constraint on the $\\Delta R_{np}$ recently. We employ a more practicable strategy currently to probe the neutron skin thickness of $^{208}$Pb based on a high linear correlation between the $\\Delta R_{np}$ and $J-a_{\\text{sym}}$, where $J$ and $a_{\\text{sym}}$ are the symmetry energy (coefficient) of nuclear matter at saturation density and of $^{208}$Pb. An accurate $J-a_{\\text{sym}}$ thus places a strong constraint on the $\\Delta R_{np}$. Compared with the parity-violating asymmetry $A_{\\text{PV}}$ in the PREX, the reliably experimental information on the $J-a_{\\text{sym}}$ is much more easily available attributed to a wealth of measured data on nuclear masses and on decay energies. The density dependence of the symmetry energy is also well constrained with the $J-a_{\\...

  3. Jefferson Lab Human Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    JLab Diversity Policies 200 Human Resources 202 Equal Employment Opportunity and Affirmative Action 203 Employment 208 Employee Performance and Conduct 209 Staff Development 210...

  4. ORISE: Human Subjects Protection

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Subjects Protection The Oak Ridge Institute for Science and Education (ORISE) performs technical assessments to assist U.S. Department of Energy (DOE) laboratories involved...

  5. On the fracture of human dentin: Is it stress-or strain-controlled?

    E-Print Network [OSTI]

    Ritchie, Robert

    On the fracture of human dentin: Is it stress- or strain-controlled? R. K. Nalla,1 J. H. Kinney,2 R information in archival literature that can be usefully used to model such fracture. In fact, although the fracture event in dentin, akin to other mineralized tissues like bone, is widely believed to be locally

  6. Identification of an octamer-binding site in the human kappa light-chain enhancer

    SciTech Connect (OSTI)

    Nelms, K.; Van Ness, B. (Institute of Human Genetics and Dept. of Biochemistry, Univ. of Minnesota, Minneapolis (MN))

    1990-07-01

    Octamer motifs contribute to the function and tissue specificity of immunoglobulin heavy- and light-chain gene promoters and the heavy-chain enhancer. A variant octamer-binding site within a conserved region of the human kappa light-chain gene enhancer which contributes to the function of this enhancer has been identified.

  7. TU-F-18A-03: Improving Tissue Segmentation for Monte Carlo Dose Calculation Using DECT Data

    SciTech Connect (OSTI)

    Di, Salvio A; Bedwani, S; Carrier, J

    2014-06-15

    Purpose: To develop a new segmentation technique using dual energy CT (DECT) to overcome limitations related to segmentation from a standard Hounsfield unit (HU) to electron density (ED) calibration curve. Both methods are compared with a Monte Carlo analysis of dose distribution. Methods: DECT allows a direct calculation of both ED and effective atomic number (EAN) within a given voxel. The EAN is here defined as a function of the total electron cross-section of a medium. These values can be effectively acquired using a calibrated method from scans at two different energies. A prior stoichiometric calibration on a Gammex RMI phantom allows us to find the parameters to calculate EAN and ED within a voxel. Scans from a Siemens SOMATOM Definition Flash dual source system provided the data for our study. A Monte Carlo analysis compares dose distribution simulated by dosxyz-nrc, considering a head phantom defined by both segmentation techniques. Results: Results from depth dose and dose profile calculations show that materials with different atomic compositions but similar EAN present differences of less than 1%. Therefore, it is possible to define a short list of basis materials from which density can be adapted to imitate interaction behavior of any tissue. Comparison of the dose distributions on both segmentations shows a difference of 50% in dose in areas surrounding bone at low energy. Conclusion: The presented segmentation technique allows a more accurate medium definition in each voxel, especially in areas of tissue transition. Since the behavior of human tissues is highly sensitive at low energies, this reduces the errors on calculated dose distribution. This method could be further developed to optimize the tissue characterization based on anatomic site.

  8. The human genome project

    SciTech Connect (OSTI)

    Yager, T.D.; Zewert, T.E.; Hood, L.E. )

    1994-04-01

    The Human Genome Project (HGP) is a coordinated worldwide effort to precisely map the human genome and the genomes of selected model organisms. The first explicit proposal for this project dates from 1985 although its foundations (both conceptual and technological) can be traced back many years in genetics, molecular biology, and biotechnology. The HGP has matured rapidly and is producing results of great significance.

  9. HUMANITIES & SOCIAL SCIENCES

    E-Print Network [OSTI]

    Yang, Eui-Hyeok

    , The Role of Technology in Film, and even the History of Aeronautics-- in each case, at the request of the students. HUMANITIES & SOCIAL SCIENCES STEVENS INSTITUTE OF TECHNOLOGY www.stevens.edu MAJORS: + History in this ever- changing, fast-paced, technology-driven world. They also investigate how human in- novation

  10. Human Functional Brain Imaging

    E-Print Network [OSTI]

    Rambaut, Andrew

    Human Functional Brain Imaging 19902009 September 2011 Portfolio Review Summary Brain Imaging #12 Dale one of our first Trustees. Understanding the brain remains one of our key strategic aims today three-fold: to identify the key landmarks and influences on the human functional brain imaging

  11. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-12-20

    The order establishes Department of Energy (DOE) procedures and responsibilities for implementing the policy and requirements set forth in 10 Code of Federal Regulations (CFR) Part 745, Protection of Human Subjects; and in DOE P 443.1A, Protection of Human Subjects, dated 12-20-07. Cancels DOE O 443.1. Canceled by DOE O 443.1B.

  12. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2000-05-15

    To establish DOE procedures and responsibilities for implementing the policy and requirements set forth in 10 CFR Part 745, Protection of Human Subjects, ad in DOE P 443.1, Policy on the Protection of Human Subjects. Cancels DOE O 1300.3. Canceled by DOE O 443.1A.

  13. Department of Humanities Program in Technology and the Humanities

    E-Print Network [OSTI]

    ; ethics in the professions; history of art and architecture; humanizing technology; information seek- ingHumanities Department of Humanities Program in Technology and the Humanities Siegel Hall 218 3301 S' Graduate Program in Technology and Humanities prepares students for careers in emerging forms of technology

  14. Integral equation models for thermoacoustic imaging of dissipative tissue

    E-Print Network [OSTI]

    Kowar, Richard

    2010-01-01

    In case of non-dissipative tissue the inverse problem of thermoacoustic imaging basically consists of two inverse problems. First, a function $\\phi$ depending on the \\emph{electromagnetic absorption function}, is estimated from one of three types of projections (spherical, circular or planar) and secondly, the \\emph{electromagnetic absorption function} is estimated from $\\phi$. In case of dissipative tissue, it is no longer possible to calculate explicitly the projection of $\\phi$ from the respective pressure data (measured by point, planar or line detectors). The goal of this paper is to derive for each of the three types of pressure data, an integral equation that allows estimating the respective projection of $\\phi$. The advantage of this approach is that all known reconstruction formulas for $\\phi$ from the respective projection can be exploited.

  15. Method of tissue repair using a composite material

    DOE Patents [OSTI]

    Hutchens, Stacy A; Woodward, Jonathan; Evans, Barbara R; O'Neill, Hugh M

    2014-03-18

    A composite biocompatible hydrogel material includes a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa. A calcium comprising salt is disposed in at least some of the pores. The porous polymer matrix can comprise cellulose, including bacterial cellulose. The composite can be used as a bone graft material. A method of tissue repair within the body of animals includes the steps of providing a composite biocompatible hydrogel material including a porous polymer matrix, the polymer matrix including a plurality of pores and providing a Young's modulus of at least 10 GPa, and inserting the hydrogel material into cartilage or bone tissue of an animal, wherein the hydrogel material supports cell colonization in vitro for autologous cell seeding.

  16. Tissue sampling methods and standards for vertebrate genomics

    E-Print Network [OSTI]

    Wong, Pamela B. Y.; Wiley, Edward O.; Johnson, Warren E.; Ryder, Oliver A.; O'Brien, Stephen; Haussler, David; Koepfli, Klaus-Peter; Houk, Marlys L.; Perelman, Polina; Mastromonaco, Gabriela; Bentley, Andrew C.; Venkatesh, Byrappa; Zhang, Ya-ping; Murphy, Robert W.; G10KCOS

    2012-07-12

    procedures in acqu from target species will facilitate these initiatives. We prov standards of acquiring and storing tissue for the Genome 2012 Wong et al.; licensee BioMed Central L Commons Attribution License (http://creativec reproduction in any medium... their Utilization to the Convention on Bio- logical Diversity, promoting genetic research toward con- serving biological diversity [[20], see [21] for text]. Accordingly, equal access and benefit-sharing should be ensured, in addition to complying with regulations...

  17. Continuous human cell lines and method of making same

    DOE Patents [OSTI]

    Stampfer, M.R.

    1985-07-01

    Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.

  18. Surface coating for prevention of metallic seed migration in tissues

    SciTech Connect (OSTI)

    Lee, Hyunseok; Park, Jong In; Lee, Won Seok; Park, Min; Son, Kwang-Jae; Bang, Young-bong; Choy, Young Bin E-mail: sye@snu.ac.kr; Ye, Sung-Joon E-mail: sye@snu.ac.kr

    2015-06-15

    Purpose: In radiotherapy, metallic implants often detach from their deposited sites and migrate to other locations. This undesirable migration could cause inadequate dose coverage for permanent brachytherapy and difficulties in image-guided radiation delivery for patients. To prevent migration of implanted seeds, the authors propose a potential strategy to use a biocompatible and tissue-adhesive material called polydopamine. Methods: In this study, nonradioactive dummy seeds that have the same geometry and composition as commercial I-125 seeds were coated in polydopamine. Using scanning electron microscopy and x-ray photoelectron spectroscopy, the surface of the polydopamine-coated and noncoated seeds was characterized. The detachment stress between the two types of seeds and the tissue was measured. The efficacy of polydopamine-coated seed was investigated through in vitro migration tests by tracing the seed location after tissue implantation and shaking for given times. The cytotoxicity of the polydopamine coating was also evaluated. Results: The results of the coating characterization have shown that polydopamine was successfully coated on the surface of the seeds. In the adhesion test, the polydopamine-coated seeds had 2.1-fold greater detachment stress than noncoated seeds. From the in vitro test, it was determined that the polydopamine-coated seed migrated shorter distances than the noncoated seed. This difference was increased with a greater length of time after implantation. Conclusions: The authors suggest that polydopamine coating is an effective technique to prevent migration of implanted seeds, especially for permanent prostate brachytherapy.

  19. Mitogen-activated protein kinase kinase 1/extracellular signal-regulated kinase (MEK-1/ERK) inhibitors sensitize reduced glucocorticoid response mediated by TNF{alpha} in human epidermal keratinocytes (HaCaT)

    SciTech Connect (OSTI)

    Onda, Kenji [Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan)]. E-mail: knjond@ps.toyaku.ac.jp; Nagashima, Masahiro [Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Kawakubo, Yo [Department of Dermatology, Teikyo University School of Medicine, Ichihara Hospital, Chiba (Japan); Inoue, Shota [Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Hirano, Toshihiko [Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Oka, Kitaro [Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan)

    2006-12-08

    Glucocorticoids (GCs) are essential drugs administered topically or systematically for the treatment of autoimmune skin diseases such as pemphigus. However, a certain proportion of patients does not respond well to GCs. Although studies on the relationship between cytokines and GC insensitivity in local tissues have attracted attention recently, little is known about the underlying mechanism(s) for GC insensitivity in epidermal keratinocytes. Here, we report that tumor necrosis factor (TNF) {alpha} reduces GC-induced transactivation of endogenous genes as well as a reporter plasmid which contains GC responsive element (GRE) in human epidermal keratinocyte cells (HaCaT). The GC insensitivity by TNF{alpha} was not accompanied by changes in mRNA expressions of GR isoforms ({alpha} or {beta}). However, we observed that mitogen-activated protein kinase kinase-1/extracellular signal-regulated kinase (MEK-1/ERK) inhibitors (PD98059 and U0126) significantly sensitized the GC-induced transactivation of anti-inflammatory genes (glucocorticoid-induced leucine zipper (GILZ) and mitogen-activated protein kinase phosphatase (MKP)-1) and FK506 binding protein (FKBP) 51 gene in the presence of TNF{alpha}. Additionally, we observed that TNF{alpha} reduced prednisolone (PSL)-dependent nuclear translocation of GR, which was restored by pre-treatment of MEK-1 inhibitors. This is the first study demonstrating a role of the MEK-1/ERK cascade in TNF{alpha}-mediated GC insensitivity. Our data suggest that overexpression of TNF{alpha} leads to topical GC insensitivity by reducing GR nuclear translocation in keratinocytes, and our findings also suggest that inhibiting the MEK-1/ERK cascade may offer a therapeutic potential for increasing GC efficacy in epidermis where sufficient inflammatory suppression is required.

  20. Telerobotic system concept for real-time soft-tissue imaging during radiotherapy beam delivery

    SciTech Connect (OSTI)

    Schlosser, Jeffrey; Salisbury, Kenneth; Hristov, Dimitre [Department of Mechanical Engineering, Stanford University, Stanford, California 94305 (United States); Department of Computer Science and Department of Surgery, Stanford University, Stanford, California 94305 (United States); Department of Radiation Oncology, Stanford University, Stanford, California 94305 (United States)

    2010-12-15

    Purpose: The curative potential of external beam radiation therapy is critically dependent on having the ability to accurately aim radiation beams at intended targets while avoiding surrounding healthy tissues. However, existing technologies are incapable of real-time, volumetric, soft-tissue imaging during radiation beam delivery, when accurate target tracking is most critical. The authors address this challenge in the development and evaluation of a novel, minimally interfering, telerobotic ultrasound (U.S.) imaging system that can be integrated with existing medical linear accelerators (LINACs) for therapy guidance. Methods: A customized human-safe robotic manipulator was designed and built to control the pressure and pitch of an abdominal U.S. transducer while avoiding LINAC gantry collisions. A haptic device was integrated to remotely control the robotic manipulator motion and U.S. image acquisition outside the LINAC room. The ability of the system to continuously maintain high quality prostate images was evaluated in volunteers over extended time periods. Treatment feasibility was assessed by comparing a clinically deployed prostate treatment plan to an alternative plan in which beam directions were restricted to sectors that did not interfere with the transabdominal U.S. transducer. To demonstrate imaging capability concurrent with delivery, robot performance and U.S. target tracking in a phantom were tested with a 15 MV radiation beam active. Results: Remote image acquisition and maintenance of image quality with the haptic interface was successfully demonstrated over 10 min periods in representative treatment setups of volunteers. Furthermore, the robot's ability to maintain a constant probe force and desired pitch angle was unaffected by the LINAC beam. For a representative prostate patient, the dose-volume histogram (DVH) for a plan with restricted sectors remained virtually identical to the DVH of a clinically deployed plan. With reduced margins, as would be enabled by real-time imaging, gross tumor volume coverage was identical while notable reductions of bladder and rectal volumes exposed to large doses were possible. The quality of U.S. images obtained during beam operation was not appreciably degraded by radiofrequency interference and 2D tracking of a phantom object in U.S. images obtained with the beam on/off yielded no significant differences. Conclusions: Remotely controlled robotic U.S. imaging is feasible in the radiotherapy environment and for the first time may offer real-time volumetric soft-tissue guidance concurrent with radiotherapy delivery.

  1. Plasmonic light-sensitive skins of nanocrystal monolayers This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Demir, Hilmi Volkan

    Plasmonic light-sensitive skins of nanocrystal monolayers This article has been downloaded from to the journal homepage for more Home Search Collections Journals About Contact us My IOPscience #12;IOP light-sensitive skins of nanocrystal monolayers Shahab Akhavan1, Kivanc Gungor1, Evren Mutlugun1

  2. Evaluating self-reported pressure ulcer prevention measures in persons with1 spinal cord injury using the revised Skin Management Needs Assessment2

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    using the revised Skin Management Needs Assessment2 Checklist : reliability study3 Glis Anthony, MD 1, Centre Hospitalier Universitaire de20 Bordeaux, France21 22 Corresponding author:23 Dr. Anthony GELIS, M: to translate, evaluate the reliability and cross-culturally adapt the Skin2 Management Needs Assessment

  3. Comparison of blood flow and cell function in ischemic skin flaps

    SciTech Connect (OSTI)

    Bean, D.; Rees, R.S.; O'Leary, J.P.; Lynch, J.B.

    1984-07-01

    Cellular function and blood flow in acute, steroid-treated, and surgically delayed random skin flaps have been examined. In these studies, the period following flap elevation could be divided into early (0-2 hr), intermediate (4-6 hr), and late (12 hr) periods of ischemia, based on the cutaneous blood flow and cellular function measured by thallium-201 uptake. There was a close correlation between blood flow and cellular function during the early period of ischemia which became worse with time. Blood flow studies demonstrated a significant difference between the early and intermediate periods of ischemia which was abolished by surgical delay. Improvement in cellular function was accomplished by improved blood flow in the surgically delayed flaps, while steroid-treated flaps enhanced cellular metabolism by another mechanism. Cellular function approximated blood flow during the early and immediate period of ischemia. Steroids may augment cellular function without improving blood flow, while surgical delay improves cellular function by improving blood flow.

  4. The neutron skin in neutron-rich nuclei at Jefferson Lab

    SciTech Connect (OSTI)

    Dalton, Mark M.

    2013-11-01

    The Jefferson Lab program to measure the symmetry energy of neutron-rich nuclear matter, using precision electroweak methods, is progressing well. The initial measurement by the PREX experiment, leading to a 2-sigma determination of the "neutron skin" in {sup 208}Pb , has been published. Design and preparation for a further, more-precise measurement on {sup 208}Pb is progressing well and there is general acceptance of the great advantage to a further measurement on {sup 48}Ca . The surprising ancillary result that the beam-normal single-spin asymmetry for {sup 208}Pb is consistent with zero is also now in the literature. This paper will discuss the current experimental situation of the program.

  5. Method of forming a continuous polymeric skin on a cellular foam material

    DOE Patents [OSTI]

    Duchane, David V. (Los Alamos, NM); Barthell, Barry L. (Los Alamos, NM)

    1985-01-01

    Hydrophobic cellular material is coated with a thin hydrophilic polymer skin which stretches tightly over the outer surface of the foam but which does not fill the cells of the foam, thus resulting in a polymer-coated foam structure having a smoothness which was not possible in the prior art. In particular, when the hydrophobic cellular material is a specially chosen hydrophobic polymer foam and is formed into arbitrarily chosen shapes prior to the coating with hydrophilic polymer, inertial confinement fusion (ICF) targets of arbitrary shapes can be produced by subsequently coating the shapes with metal or with any other suitable material. New articles of manufacture are produced, including improved ICF targets, improved integrated circuits, and improved solar reflectors and solar collectors. In the coating method, the cell size of the hydrophobic cellular material, the viscosity of the polymer solution used to coat, and the surface tensin of the polymer solution used to coat are all very important to the coating.

  6. Neutron-skin thickness from the study of the anti-analog giant dipole resonance

    SciTech Connect (OSTI)

    Krasznahorkay, A.; Stuhl, L.; Csatlos, M.; Algora, A.; and others

    2012-10-20

    The {gamma}-decay of the anti-analog of the giant dipole resonance (AGDR) to the isobaric analog state has been measured following the p({sup 124}Sn,n) reaction at a beam energy of 600 MeV/nucleon. The energy of the transition was also calculated with state-of-the-art self-consistent relativistic random-phase approximation (RPA) and turned out to be very sensitive to the neutronskin thickness ({Delta}R{sub pn}). By comparing the theoretical results with the measured one, the {Delta}R{sub pn} value for {sup 124}Sn was deduced to be 0.21 {+-} 0.07 fm, which agrees well with the previous results. The present method offers new possibilities for measuring the neutron-skin thicknesses of very exotic isotopes.

  7. Analysis of Conductor Impedances Accounting for Skin Effect and Nonlinear Permeability

    SciTech Connect (OSTI)

    Perkins, M P; Ong, M M; Brown, C G; Speer, R D

    2011-07-20

    It is often necessary to protect sensitive electrical equipment from pulsed electric and magnetic fields. To accomplish this electromagnetic shielding structures similar to Faraday Cages are often implemented. If the equipment is inside a facility that has been reinforced with rebar, the rebar can be used as part of a lighting protection system. Unfortunately, such shields are not perfect and allow electromagnetic fields to be created inside due to discontinuities in the structure, penetrations, and finite conductivity of the shield. In order to perform an analysis of such a structure it is important to first determine the effect of the finite impedance of the conductors used in the shield. In this paper we will discuss the impedances of different cylindrical conductors in the time domain. For a time varying pulse the currents created in the conductor will have different spectral components, which will affect the current density due to skin effects. Many construction materials use iron and different types of steels that have a nonlinear permeability. The nonlinear material can have an effect on the impedance of the conductor depending on the B-H curve. Although closed form solutions exist for the impedances of cylindrical conductors made of linear materials, computational techniques are needed for nonlinear materials. Simulations of such impedances are often technically challenging due to the need for a computational mesh to be able to resolve the skin depths for the different spectral components in the pulse. The results of such simulations in the time domain will be shown and used to determine the impedances of cylindrical conductors for lightning current pulses that have low frequency content.

  8. Engineered Contractile Skeletal Muscle Tissue on a Microgrooved Methacrylated Gelatin Substrate

    E-Print Network [OSTI]

    Hosseini, Vahid

    To engineer tissue-like structures, cells must organize themselves into three-dimensional (3D) networks that mimic the native tissue microarchitecture. Microfabricated hydrogel substrates provide a potentially useful ...

  9. Tissue Engineering Approaches for Studying the Effect of Biochemical and Physiological Stimuli on Cell Behavior

    E-Print Network [OSTI]

    Jimenez Vergara, Andrea

    2012-10-19

    Tissue engineering (TE) approaches have emerged as an alternative to traditional tissue and organ replacements. The aim of this work was to contribute to the understanding of the effects of cell-material and endothelial ...

  10. Tissue spectroscopic characterization based on fluorescence, second harmonic generation, and reflected light

    E-Print Network [OSTI]

    Laiho, Lily H., 1973-

    2004-01-01

    The diagnosis of many diseases often requires a histological analysis of tissues. Histology analysis compares the microscopic structure of a tissue specimen with an image database containing known physiological and ...

  11. Polymer-tethered epidermal growth factor as an inductive biomaterial surface for connective tissue progenitors

    E-Print Network [OSTI]

    Fan, Vivian H. (Vivian Hanbing)

    2006-01-01

    Connective tissue progenitors (CTP) can act as a pluripotent source of reparative cells during injury and therefore have great potential in regenerative medicine and tissue engineering. However, the response of CTP to most ...

  12. Confocal Microscopy and Nuclear Segmentation Algorithm for Quantitative Imaging of Epithelial Tissue

    E-Print Network [OSTI]

    Harris, Meagan Alyssa

    2015-07-08

    for imaging epithelial tissues and an image processing algorithm for segmentation of epithelial nuclei. A rapid beam and stage scanning combination was used to acquire fluorescence confocal images of cellular and tissue features along the length of excised...

  13. Development of a bioengineered tissue model and its application in the investigation of the depth

    E-Print Network [OSTI]

    Bustamante, Fabin E.

    Development of a bioengineered tissue model and its application in the investigation of the depth the development of a bioengineered connective tissue model fabricated by the combination of scaffolding and cross

  14. Dynamic Skin Triangulation HoLun Cheng y , Tamal K. Dey z , Herbert Edelsbrunner x and John Sullivan {

    E-Print Network [OSTI]

    Cheng, Ho-lun, "Alan"

    that grow and shrink with time. An example is the boundary between the solid and the liquid portions the nu cleation, growth and coarsening stages [1]. Moving bound aries also arise naturally in mold the finite collection by convex combina tion and shrinking. The skin surface is the envelope of this family

  15. IEEE TRANSACTIONS ON MAGNETICS, VOL. 36, NO. 1, JANUARY 2000 281 Thin-Skin Eddy-Current Interaction with

    E-Print Network [OSTI]

    Bowler, John R.

    IEEE TRANSACTIONS ON MAGNETICS, VOL. 36, NO. 1, JANUARY 2000 281 Thin-Skin Eddy-Current Interaction with Semielliptical and Epicyclic Cracks J. R. Bowler, Member, IEEE, and N. Harfield Abstract--Eddy-current probe current, nondestructive evaluation. I. INTRODUCTION IN EDDY-CURRENT nondestructive evaluation, cracks

  16. A Three Level Finite Difference Scheme for Solving the Pennes' Bioheat Transfer in a TripleLayered Skin

    E-Print Network [OSTI]

    Zhang, Jun

    . A convergence theorem is obtained by the discrete energy method, implying that the scheme is un conditionally the discrete energy method, we establish a convergence theorem for the finite difference scheme, which shows stable. Numerical results for thermal analysis of a skin composed of epidermis, dermis and subcutaneous

  17. Effect of dietary polyunsaturated fatty acid and related nutrients on plasma lipids, and skin and hair coat condition in canines

    E-Print Network [OSTI]

    Hester, Shaleah Lynnae

    2004-11-15

    A study was performed to investigate the effect of diet modifications on skin and hair coat condition in canines. The study included 24 normal adult dogs fed a baseline diet (Ol'Roy[trademark]), during an acclimation period of 12 wk (Phase I). Nine...

  18. Effect of dietary polyunsaturated fatty acids and related nutrients on sebum lipids, and skin and hair coat condition in canines

    E-Print Network [OSTI]

    Kirby, Naomi Anne

    2005-02-17

    feeding period of Phase II, test results revealed statistically significant increases in both CE and CE/WD, and a prolonged growth of hair follicles in the anagen phase of diet B dogs, which are both consistent with improved skin and hair coat scores....

  19. Nuclear matter symmetry energy and the neutron skin thickness of heavy nuclei RID A-2398-2009

    E-Print Network [OSTI]

    Chen, LW; Ko, Che Ming; Li, Ba.

    2005-01-01

    Correlations between the thickness of the neutron skin in finite nuclei and the nuclear matter symmetry energy are studied in the Skyrme Hartree-Fock model. From the most recent analysis of the isospin diffusion data in heavy-ion collisions based...

  20. Semi-analytical methods for the analysis and interpretation of well test data distorted by wellbore storage and skin effects

    E-Print Network [OSTI]

    Prachumchon, Sompong

    1996-01-01

    Our objective is to develop approximations of the pressure-time behavior for use in analyzing the pressure response of a well in an infinite-acting reservoir influenced by wellbore storage and skin effects. Our resulting approximate models are semi-analytical...

  1. Antagonistic effect of indigenous skin bacteria of brook charr (Salvelinus fontinalis) against Flavobacterium columnare and F. psychrophilum

    E-Print Network [OSTI]

    Bernatchez, Louis

    in aquaculture are strongly needed. The development of probiotics appears to be one of the most promising ways psychrophilum (Bernardet and Bowman, 2006). Skin microflora was targeted as a potential source of probiotics August 2011 Accepted 1 September 2011 Keywords: Probiotics Flavobacterium Salvelinus fontinalis

  2. Land surface skin temperatures from a combined analysis of microwave and infrared satellite observations for an all-weather

    E-Print Network [OSTI]

    Aires, Filipe

    Land surface skin temperatures from a combined analysis of microwave and infrared satellite Microwave/Imager (SSM/I) and International Satellite Cloud Climatology Project (ISCCP) data. In the absence all the expected variations with solar flux, soil characteristics, and cloudiness. During daytime

  3. In vivo imaging of immune cell dynamics in skin in response to zinc-oxide nanoparticle exposure

    E-Print Network [OSTI]

    Boppart, Stephen

    in cosmetic and sunscreen products which are applied topically to the skin. Despite their widespread use of nanosized particles in titanium dioxide- and zinc oxide-based sunscreens," J. Am. Acad. Dermatol. 61(4), 685 by cosmetic pigments and sunscreen agents under solar exposure and artificial UV illumination," J. Oleo Sci

  4. Special Issue on Human Computing

    E-Print Network [OSTI]

    Nijholt, Anton

    The seven articles in this special issue focus on human computing. Most focus on two challenging issues in human computing, namely, machine analysis of human behavior in group interactions and context-sensitive modeling.

  5. carleton.ca Human Rights

    E-Print Network [OSTI]

    Dawson, Jeff W.

    , including social movements, domestic legislation, international statutes, and human rights declarations the development of historical and contemporary social movements, and the effects of global capitalismcarleton.ca Human Rights #12;Human rights contribute, through theory and practice, to people

  6. Human Reliability Program Overview

    SciTech Connect (OSTI)

    Bodin, Michael

    2012-09-25

    This presentation covers the high points of the Human Reliability Program, including certification/decertification, critical positions, due process, organizational structure, program components, personnel security, an overview of the US DOE reliability program, retirees and academia, and security program integration.

  7. KRFTWRK Global Human Electricity

    E-Print Network [OSTI]

    Prohaska, Rainer

    2009-01-01

    Power Network 2.1.1 Virtual Power Plants The Global Powernetwork, based on "Virtual Power Plants", called "VPP". A "participant runs a virtual human power plant. Per every "

  8. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-12-20

    The Policy is to establish DOE-specific principles for the protection of human subjects involved in DOE research. Cancels DOE P 443.1. Canceled by DOE O 443.1B

  9. Human-spacesuit interaction :

    E-Print Network [OSTI]

    Hilbert, Alexandra Marie

    2015-01-01

    Extravehicular activities (EVA), or space walks, are a critical and complex aspect of human spaceflight missions. To prepare for safe and successful execution of the required tasks, astronauts undergo extensive training ...

  10. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2000-05-15

    The purpose of this Policy is to establish DOE-specific policy for the protection of human subjects involved in DOE research. Canceled by DOE P 443.1A.

  11. Human Resource Management Delegation

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1996-06-28

    The notice is to clarifies and updates existing Human Resource Management Delegation Authorities and the levels to which they are delegated. Expired 6-28-97. Does not cancel any directives.

  12. COMPARATIVE COMPUTATIONAL MODELING OF AIRFLOWS AND VAPOR DOSIMETY IN THE RESPIRATORY TRACTS OF RAT, MONKEY, AND HUMAN

    SciTech Connect (OSTI)

    Corley, Richard A.; Kabilan, Senthil; Kuprat, Andrew P.; Carson, James P.; Minard, Kevin R.; Jacob, Rick E.; Timchalk, Charles; Glenny, Robb W.; Pipavath, Sudhaker; Cox, Timothy C.; Wallis, Chris; Larson, Richard; Fanucchi, M.; Postlewait, Ed; Einstein, Daniel R.

    2012-07-01

    Coupling computational fluid dynamics (CFD) with physiologically based pharmacokinetic (PBPK) models is useful for predicting site-specific dosimetry of airborne materials in the respiratory tract and elucidating the importance of species differences in anatomy, physiology, and breathing patterns. Historically, these models were limited to discrete regions of the respiratory system. CFD/PBPK models have now been developed for the rat, monkey, and human that encompass airways from the nose or mouth to the lung. A PBPK model previously developed to describe acrolein uptake in nasal tissues was adapted to the extended airway models as an example application. Model parameters for each anatomic region were obtained from the literature, measured directly, or estimated from published data. Airflow and site-specific acrolein uptake patterns were determined under steadystate inhalation conditions to provide direct comparisons with prior data and nasalonly simulations. Results confirmed that regional uptake was dependent upon airflow rates and acrolein concentrations with nasal extraction efficiencies predicted to be greatest in the rat, followed by the monkey, then the human. For human oral-breathing simulations, acrolein uptake rates in oropharyngeal and laryngeal tissues were comparable to nasal tissues following nasal breathing under the same exposure conditions. For both breathing modes, higher uptake rates were predicted for lower tracheo-bronchial tissues of humans than either the rat or monkey. These extended airway models provide a unique foundation for comparing dosimetry across a significantly more extensive range of conducting airways in the rat, monkey, and human than prior CFD models.

  13. Tissue Classification using Cluster Features for Lesion Detection in Digital Cervigrams

    E-Print Network [OSTI]

    Huang, Xiaolei

    Tissue Classification using Cluster Features for Lesion Detection in Digital Cervigrams Xiaolei, tissue classification, lesion detection, digital cervigrams, cervical cancer 1. INTRODUCTION To make of different tissue types in digitized uterine cervix images using mean-shift clustering and support vector

  14. Enhanced Background Rejection in Thick Tissue with Differential-Aberration Two-Photon Microscopy

    E-Print Network [OSTI]

    to superficial tissue damage, then the power density of the ballistic light near the surface can be so high and the ballistic light so weak that the power density of the ballistic light cannot compete at the beam focus can lead to significant power densities near the tissue surface. If the tissue

  15. Needle Steering in Biological Tissue using Ultrasound-based Online Curvature Estimation

    E-Print Network [OSTI]

    Alterovitz, Ron

    in a homogenous gelatin phantom, a two-layer gelatin phantom, and a biological tissue phantom composed of a gelatin layer and in vitro chicken tissue. In all experiments, virtual obstacles and targets move in order phantom composed of gelatin and biological tissue. Steerable needles have been introduced to enable

  16. Automatic classification of mammography reports by BI-RADS breast tissue composition class

    E-Print Network [OSTI]

    Rubin, Daniel L.

    Automatic classification of mammography reports by BI-RADS breast tissue composition class Bethany tissue composition partially predicts breast cancer risk, classification of mammography reports by breast for using the unstructured text of mammography reports to classify them into BI-RADS breast tissue

  17. Scanning thermoacoustic tomography in biological tissue Geng Ku and Lihong V. Wanga)

    E-Print Network [OSTI]

    Wang, Lihong

    Scanning thermoacoustic tomography in biological tissue Geng Ku and Lihong V. Wanga) Optical-induced thermoacoustic tomography was explored to image biological tissue. Short microwave pulses irradiated tissue to generate acoustic waves by thermoelastic expansion. The microwave-induced thermoacoustic waves were

  18. A continuous model for an arterial tissue, incorporating remodeling and volumetric growth

    E-Print Network [OSTI]

    Eindhoven, Technische Universiteit

    A continuous model for an arterial tissue, incorporating remodeling and volumetric growth Fons van. Both components are incompressible, but the tissue as a whole can show volumetric growth due laws of mass and momentum. Keywords : Arterial tissue, volumetric growth, straininduced orientation

  19. SU-E-T-68: Clinical Implementation of Total Skin Electron Beam Therapy: A New- York Presbyterian Hospital Experience

    SciTech Connect (OSTI)

    Afghan, M; Shih, R; Chen, H

    2014-06-01

    Purpose: Total skin electron beam therapy (TSET) is used in the treatment of rare skin diseases such as mycosis fungoides, the most common type of cutaneous T-cell lymphoma. We report our experience with clinical implementation of TSET. Methods: A modified six-dual-field irradiation technique was chosen to deliver TSET. A Varian Trilogy linear accelerator with a nominal 6 MeV beam using high dose rate total skin electron mode (HDTSe) was employed. The recommendations of AAPM task group report 23 were followed for the commissioning. An acrylic plate (energy degrader) of 3.2 mm depth was mounted on the HDTSe applicator. The nominal source to skin distance was set at 450 cm. The optimum tilt angle of the gantry was determined using NACP-02 ionization chamber embedded in certified therapy grade solid water. Percent depth dose measurements were performed using ionization chamber and radiochromic films embedded in solid water and anthropomorphic phantom. For absolute dose measurements, TG-51 formalism was employed. The dose distribution on the entire skin was measured by irradiating the anthropomorphic phantom, with TLDs attached, mimicking the real treatment. Results: The 3.2 mm acrylic plate mounted on the HDTSe applicator degraded the energy of the electron beam to 4.1 MeV in the treatment plane, located at an SSD of 450 cm. The optimum tilt angle was found to be 20. A single-dual field had a longitudinal uniformity, measured at a depth of dose maximum, of 7% over a length of about 200 cm. For the entire treatment the multiplication factor was found to be 2.86. On the surface of the phantom, the dose varied from 108% to 93% of the prescription dose. Conclusion: We have successfully commissioned TSET meeting the guidelines of the TG report 23, and treated our first patient on February 25, 2014.

  20. The Evolution of Human Cooperation

    E-Print Network [OSTI]

    Gintis, Herbert; Doebeli, Michael; Flack, Jessica

    2012-01-01

    684 Gintis, H. 2011. The Evolution of Human Cooperation.misunderstandings about cultural evolution. Human Nat. 19,Feldman, M. , 1981. Cultural Evolution. Princeton University

  1. ORISE: Protecting Human Subjects Website

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protecting Human Subjects Website Institutions that engage in human subjects research are required by federal policy to establish an institutional review board (IRB) to ensure that...

  2. Photoacoustic computed tomography in biological tissues: algorithms and breast imaging

    E-Print Network [OSTI]

    Xu, Minghua

    2004-11-15

    can be written in the frequency domain [23-26] as: )~~ 00)(30 rminute??? minute poutk V , (1.8) where V minute is the volume of the source )0 rminute ; and )~ 0)( routk is a Green?s function: 0 0 0 ) 4 )),(~ r rrr - -=minute ikG out k , (1... in biological tissues. The reduced or effective scattering coefficient is described by )ss - , where s and g are the scattering coefficient and the anisotropy factor, respectively. Typically 100s cm?1 and g ~ 0.9 in the visible to near-IR region [18...

  3. Trace element concentrations in melanotic swine

    SciTech Connect (OSTI)

    Sherman, R.M.; Parkinson, T.F.; Veit, H.P.

    1983-10-01

    A number of investigations have been made on the role of certain trace elements in oncogenesis. In prior work, it was found that manganese and zinc concentrations in human skin cancers differed markedly from the values in normal tissue./sub 1/ The purpose of the present research was to determine trace element concentrations in skin and other tissues of normal and melanotic miniature swine. In-vivo determinations of skin tissue were carried out using x-ray fluorescence analysis (XRFA) while tissue biopsies were measured using instrumental neutron activation analysis (INAA). If significant differences between normal and cancerous skin tissues can be established, the former method should prove valuable as a rapid noninvasive diagnostic method.

  4. The Human Genome From human genome to other

    E-Print Network [OSTI]

    Linial, Michal

    The Human Genome Project From human genome to other genomes and to gene function June 2000 From genome to health Structural Genomics initiative #12;What is the Human Genome Project? U.S. govt that arise from genome research #12;The Human Genome Project Project began in 1990 as a $3 billion, 15-year

  5. Policy on Human Subjects Research Policy on Human Subjects

    E-Print Network [OSTI]

    Sridhar, Srinivas

    Policy on Human Subjects Research 10/15/2014 Policy on Human Subjects Research I. Purpose and Scope ethical standards for the protection of human subjects, consistent with the principles of the Nuremberg Code and the Belmont Report. Accordingly, the University has established the Office of Human Subject

  6. Regulation of biological tissue mineralization through post-nucleation shielding

    E-Print Network [OSTI]

    Joshua C. Chang; Robert M. Miura

    2015-01-29

    In vertebrates, insufficient availability of calcium and phosphate ions in extracellular fluids leads to loss of bone density and neuronal hyper-excitability. To counteract this problem, calcium ions are present at high concentrations throughout body fluids -- at concentrations exceeding the saturation point. This condition leads to the opposite situation where unwanted mineral sedimentation may occur. Remarkably, ectopic or out-of-place sedimentation into soft tissues is rare, in spite of the thermodynamic driving factors. This fortunate fact is due to the presence of auto-regulatory proteins that are found in abundance in bodily fluids. Yet, many important inflammatory disorders such as atherosclerosis and osteoarthritis are associated with this undesired calcification. Hence, it is important to gain an understanding of the regulatory process and the conditions under which it can go awry. In this Letter, we use ideas from mean-field classical nucleation theory to study the regulation of sedimentation of calcium phosphate salts in biological tissues through the mechanism of post-nuclear shielding of nascent mineral particles by binding proteins. A critical concentration of regulatory protein is identified as a function of the physical parameters that describe the system.

  7. Use of proton beams with breast prostheses and tissue expanders

    SciTech Connect (OSTI)

    Moyers, Michael F.; Mah, Dennis; Boyer, Sean P.; Chang, Chang; Pankuch, Mark

    2014-04-01

    Since the early 2000s, a small but rapidly increasing number of patients with breast cancer have been treated with proton beams. Some of these patients have had breast prostheses or tissue expanders in place during their courses of treatment. Procedures must be implemented to plan the treatments of these patients. The density, kilovoltage x-ray computed tomography numbers (kVXCTNs), and proton relative linear stopping powers (pRLSPs) were calculated and measured for several test sample devices. The calculated and measured kVXCTNs of saline were 1% and 2.4% higher than the values for distilled water while the calculated RLSP for saline was within 0.2% of the value for distilled water. The measured kVXCTN and pRLSP of the silicone filling material for the test samples were approximately 1120 and 0.935, respectively. The conversion of kVXCTNs to pRLSPs by the treatment planning system standard tissue conversion function is adequate for saline-filled devices but for silicone-filled devices manual reassignment of the pRLSPs is required.

  8. Elevated levels of plasma Big endothelin-1 and its relation to hypertension and skin lesions in individuals exposed to arsenic

    SciTech Connect (OSTI)

    Hossain, Ekhtear; Islam, Khairul; Yeasmin, Fouzia [Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205 (Bangladesh)] [Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205 (Bangladesh); Karim, Md. Rezaul [Department of Applied Nutrition and Food Technology, Islamic University, Kushtia-7003 (Bangladesh)] [Department of Applied Nutrition and Food Technology, Islamic University, Kushtia-7003 (Bangladesh); Rahman, Mashiur; Agarwal, Smita; Hossain, Shakhawoat; Aziz, Abdul; Al Mamun, Abdullah; Sheikh, Afzal; Haque, Abedul; Hossain, M. Tofazzal [Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205 (Bangladesh)] [Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205 (Bangladesh); Hossain, Mostaque [Department of Medicine, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka (Bangladesh)] [Department of Medicine, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka (Bangladesh); Haris, Parvez I. [Faculty of Health and Life Sciences, De Montfort University, Leicester, LE1 9BH (United Kingdom)] [Faculty of Health and Life Sciences, De Montfort University, Leicester, LE1 9BH (United Kingdom); Ikemura, Noriaki; Inoue, Kiyoshi; Miyataka, Hideki; Himeno, Seiichiro [Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 7708514 (Japan)] [Laboratory of Molecular Nutrition and Toxicology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 7708514 (Japan); Hossain, Khaled, E-mail: khossain69@yahoo.com [Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205 (Bangladesh)] [Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205 (Bangladesh)

    2012-03-01

    Chronic arsenic (As) exposure affects the endothelial system causing several diseases. Big endothelin-1 (Big ET-1), the biological precursor of endothelin-1 (ET-1) is a more accurate indicator of the degree of activation of the endothelial system. Effect of As exposure on the plasma Big ET-1 levels and its physiological implications have not yet been documented. We evaluated plasma Big ET-1 levels and their relation to hypertension and skin lesions in As exposed individuals in Bangladesh. A total of 304 study subjects from the As-endemic and non-endemic areas in Bangladesh were recruited for this study. As concentrations in water, hair and nails were measured by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). The plasma Big ET-1 levels were measured using a one-step sandwich enzyme immunoassay kit. Significant increase in Big ET-1 levels were observed with the increasing concentrations of As in drinking water, hair and nails. Further, before and after adjusting with different covariates, plasma Big ET-1 levels were found to be significantly associated with the water, hair and nail As concentrations of the study subjects. Big ET-1 levels were also higher in the higher exposure groups compared to the lowest (reference) group. Interestingly, we observed that Big ET-1 levels were significantly higher in the hypertensive and skin lesion groups compared to the normotensive and without skin lesion counterpart, respectively of the study subjects in As-endemic areas. Thus, this study demonstrated a novel doseresponse relationship between As exposure and plasma Big ET-1 levels indicating the possible involvement of plasma Big ET-1 levels in As-induced hypertension and skin lesions. -- Highlights: ? Plasma Big ET-1 is an indicator of endothelial damage. ? Plasma Big ET-1 level increases dose-dependently in arsenic exposed individuals. ? Study subjects in arsenic-endemic areas with hypertension have elevated Big ET-1 levels. ? Study subjects with arsenic-induced skin lesions show elevated plasma Big ET-1 levels. ? Arsenic-induced hypertension and skin lesions may be linked to plasma Big ET-1 levels.

  9. CONSTRUCTING VIRTUAL HUMAN LIFE SIMULATIONS

    E-Print Network [OSTI]

    Kallmann, Marcelo

    , Virtual Environments, Behavioral Animation, Object Interaction, Python. 1. INTRODUCTION Virtual humanCONSTRUCTING VIRTUAL HUMAN LIFE SIMULATIONS Marcelo Kallmann, Etienne de Sevin and Daniel Thalmann human life simulations. Our main goal is to have virtual human actors living and working autonomously

  10. Human MSH2 protein

    DOE Patents [OSTI]

    de la Chapelle, Albert (Helsingfors, FI); Vogelstein, Bert (Baltimore, MD); Kinzler, Kenneth W. (Baltimore, MD)

    1997-01-01

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error.sup.+ (RER.sup.+) tumor cells.

  11. Human MSH2 protein

    DOE Patents [OSTI]

    Chapelle, A. de la; Vogelstein, B.; Kinzler, K.W.

    1997-01-07

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error{sup +} (RER{sup +}) tumor cells. 19 figs.

  12. Human Genome: DOE Origins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (JournalvivoHigh energyHighlandWorkshop-SummerHow is theHughHumanHuman Genome

  13. Estimation of neutrophil infiltration into hairless guinea pig skin treated with 2,2' -dichlorodiethyl sulfide

    SciTech Connect (OSTI)

    Bongiovanni, R.; Millard, C.B.; Schulz, S.M.; Romano, J.M.

    1993-05-13

    Despite growing acceptance of the hairless guinea pig (HPG) for evaluating sulfur mustard (2,2'dichlorodiethylsulfide, HD) skin injury, there are presently few antivesicant drug assessment endpoints validated in vivo for this model. We measured the activity of myeloperoxidase (MPO) to characterize the dose- and time-dependence of polymorphonuclear leukocyte (PMN) infiltration during development of the HD lesion. Biopsies were obtained from the dorsal thoracic-lumbar area of HGPs at successive 3 hr time intervals for up to 24 hrs following controlled exposure to either 5, 7, 8 or 10 min HD vapor. The presence of PMNs, as judged by MPO levels, peaked at 9 hrs irrespective of total HD vapor dose. The maximum response was a 20-fold increase compared to unexposed control sites at 9 hrs following 10 min HD vapor. This time period coincides with epidermal detachment characterized previously by electron microscopy in the HGP. By 24 hrs post-exposure, the MPO levels subsided markedly (2-fold compared to controls). These results suggest that PMNs participate in the HGP cutaneous inflammatory response following exposure to HD and that MPO may be a useful biological marker for evaluating putative antivesicants.

  14. Isoproterenol effects evaluated in heart slices of human and rat in comparison to rat heart in vivo

    SciTech Connect (OSTI)

    Herrmann, Julia E.; Heale, Jason; Bieraugel, Mike; Ramos, Meg [Drug Safety Evaluation, Allergan Inc., 2525 Dupont Dr, Irvine, CA 92612 (United States); Fisher, Robyn L. [Vitron Inc., Tucson, AZ (United States); Vickers, Alison E.M., E-mail: vickers_alison@allergan.com [Drug Safety Evaluation, Allergan Inc., 2525 Dupont Dr, Irvine, CA 92612 (United States)

    2014-01-15

    Human response to isoproterenol induced cardiac injury was evaluated by gene and protein pathway changes in human heart slices, and compared to rat heart slices and rat heart in vivo. Isoproterenol (10 and 100 ?M) altered human and rat heart slice markers of oxidative stress (ATP and GSH) at 24 h. In this in vivo rat study (0.5 mg/kg), serum troponin concentrations increased with lesion severity, minimal to mild necrosis at 24 and 48 h. In the rat and the human heart, isoproterenol altered pathways for apoptosis/necrosis, stress/energy, inflammation, and remodeling/fibrosis. The rat and human heart slices were in an apoptotic phase, while the in vivo rat heart exhibited necrosis histologically and further progression of tissue remodeling. In human heart slices genes for several heat shock 70 kD members were altered, indicative of stress to mitigate apoptosis. The stress response included alterations in energy utilization, fatty acid processing, and the up-regulation of inducible nitric oxide synthase, a marker of increased oxidative stress in both species. Inflammation markers linked with remodeling included IL-1?, Il-1?, IL-6 and TNF? in both species. Tissue remodeling changes in both species included increases in the TIMP proteins, inhibitors of matrix degradation, the gene/protein of IL-4 linked with cardiac fibrosis, and the gene Ccl7 a chemokine that induces collagen synthesis, and Reg3b a growth factor for cardiac repair. This study demonstrates that the initial human heart slice response to isoproterenol cardiac injury results in apoptosis, stress/energy status, inflammation and tissue remodeling at concentrations similar to that in rat heart slices. - Highlights: Human response to isoproterenol induced cardiac injury evaluated in heart slices. Isoproterenol altered apoptosis, energy, inflammation and remodeling pathways. Human model verified by comparison to rat heart slices and rat heart in vivo. Human and rat respond to isoproterenol at similar concentrations in vitro.

  15. Coordination-resolved local bond contraction and electron binding-energy entrapment of Si atomic clusters and solid skins

    SciTech Connect (OSTI)

    Bo, Maolin; Huang, Yongli; Zhang, Ting [Key Laboratory of Low-Dimensional Materials and Application Technologies, Xiangtan University, Hunan 411105 (China); Wang, Yan, E-mail: ywang8@hnust.edu.cn, E-mail: ecqsun@ntu.edu.sg [Key Laboratory of Low-Dimensional Materials and Application Technologies, Xiangtan University, Hunan 411105 (China); School of Information and Electronic Engineering, Hunan University of Science and Technology, Hunan 411201 (China); Zhang, Xi [School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798 (Singapore); Li, Can [Center for Coordination Bond Engineering, School of Materials Science and Engineering, China Jiliang University, Hangzhou 330018 (China); Sun, Chang Q., E-mail: ywang8@hnust.edu.cn, E-mail: ecqsun@ntu.edu.sg [Key Laboratory of Low-Dimensional Materials and Application Technologies, Xiangtan University, Hunan 411105 (China); School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798 (Singapore); Center for Coordination Bond Engineering, School of Materials Science and Engineering, China Jiliang University, Hangzhou 330018 (China)

    2014-04-14

    Consistency between x-ray photoelectron spectroscopy measurements and density-function theory calculations confirms our bond order-length-strength notation-incorporated tight-binding theory predictions on the quantum entrapment of Si solid skin and atomic clusters. It has been revealed that bond-order deficiency shortens and strengthens the Si-Si bond, which results in the local densification and quantum entrapment of the core and valence electrons. Unifying Si clusters and Si(001) and (111) skins, this mechanism has led to quantification of the 2p binding energy of 96.089?eV for an isolated Si atom, and their bulk shifts of 2.461?eV. Findings evidence the significance of atomic undercoordination that is of great importance to device performance.

  16. Comparative Deposition of Zr95 in a Reticulo Endothelial Tumor to Normal Tissue in a Human Patient

    E-Print Network [OSTI]

    Low-Beer, B.V.

    2010-01-01

    National Laboratory Atomic Energy Commission, WashingtonW-7405-eng-48AI Vrl th the U. S. Atomic Energy Commission.

  17. Human health impacts of high altitude emissions

    E-Print Network [OSTI]

    Eastham, Sebastian D. (Sebastian David)

    2015-01-01

    Millions of deaths worldwide are attributed annually to exposure degraded surface air quality and UV-induced skin cancer. However, the focus has been on surface emissions, and the contribution of high altitude emissions ...

  18. Viscoelastic Analysis of Sandwich Beams Having Aluminum and Fiber-reinforced Polymer Skins with a Polystyrene Foam Core

    E-Print Network [OSTI]

    Roberts-Tompkins, Altramese L.

    2010-07-14

    fulfillment of the requirements for the degree of MASTER OF SCIENCE December 2009 Major Subject: Mechanical Engineering VISCOELASTIC ANALYSIS OF SANDWICH BEAMS HAVING ALUMINUM AND FIBER-REINFORCED POLYMER SKINS WITH A POLYSTYRENE FOAM... CORE A Thesis by ALTRAMESE LASH? ROBERTS-TOMPKINS Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Approved by: Chair of Committee...

  19. ENGINEERING AND HUMAN HEALTH

    E-Print Network [OSTI]

    Saskatchewan, University of

    ENGINEERING AND HUMAN HEALTH: FROM THE INSIDE OUT Engineering research on small scales could have huge health implications College of Engineering U N I V E R S I T Y O F S A S K ATC H E WA N CO L L E G 17 Alumni accolades College of Engineering THOROUGHUNIVERSITY OF SASKATCHEWAN COLLEGE OF ENGINEERING

  20. Photoacoustic and ultrasonic probing of bone density in ex-vivo animal tissues The purpose of the proposed research is to develop fundamental physical, instrumental and biological aspects of two novel

    E-Print Network [OSTI]

    Sun, Yu

    (photoacoustic probe) of bones through skin layers under various degrees of demineralization and porosity (void

  1. Raman spectroscopy of single human tumour cells exposed to ionizing radiation in vitro This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Brolo, Alexandre G.

    Raman spectroscopy of single human tumour cells exposed to ionizing radiation in vitro This article tissue samples which will survive long enough in a lab to perform a radiation experiment. Animal in non-human systems to radiation therapy patient outcomes. As such, prescribed doses for tumour control

  2. Human Investigation Committee or by the Institutional Review Board of McGill University.

    E-Print Network [OSTI]

    in masseter. We recorded electromyographic (EMG) responses in masseter under a range of skin stretch

  3. Brain Tissue Depth (mm) LightPowerDensity(mW/mm2

    E-Print Network [OSTI]

    Schnitzer, Mark

    Brain Tissue Depth (mm) LightPowerDensity(mW/mm2 ) Power Meter Tissue block Bare Fiber = 12 = 6 with the beveled cannula over CeA. d) Chart indicating estimated light power density seen at various distances from the fiber tip in mouse brain tissue when the light power density seen at the fiber tip was 7 mW (~99 mW/mm2

  4. Ecological succession and viability of human-associated microbiota on restroom surfaces

    SciTech Connect (OSTI)

    Gibbons, Sean M.; Schwartz, Tara; Fouquier, Jennifer; Mitchell, Michelle; Sangwan, Naseer; Gilbert, Jack A.; Kelley, Scott T.; Elkins, C. A.

    2014-11-14

    Human-associated bacteria dominate the built environment (BE). Following decontamination of floors, toilet seats, and soap dispensers in four public restrooms, in situ bacterial communities were characterized hourly, daily, and weekly to determine their successional ecology. The viability of cultivable bacteria, following the removal of dispersal agents (humans), was also assessed hourly. A late-successional community developed within 5 to 8 h on restroom floors and showed remarkable stability over weeks to months. Despite late-successional dominance by skin- and outdoor-associated bacteria, the most ubiquitous organisms were predominantly gut-associated taxa, which persisted following exclusion of humans. Staphylococcus represented the majority of the cultivable community, even after several hours of human exclusion. Methicillin-resistant Staphylococcus aureus (MRSA)-associated virulence genes were found on floors but were not present in assembled Staphylococcus pan-genomes. Viral abundances, which were predominantly enterophages, human papilloma virus, and herpesviruses, were significantly correlated with bacterial abundances and showed an unexpectedly low virus-to-bacterium ratio in surface-associated samples, suggesting that bacterial hosts are mostly dormant on BE surfaces.

  5. Ecological succession and viability of human-associated microbiota on restroom surfaces

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gibbons, Sean M.; Schwartz, Tara; Fouquier, Jennifer; Mitchell, Michelle; Sangwan, Naseer; Gilbert, Jack A.; Kelley, Scott T.; Elkins, C. A.

    2014-11-14

    Human-associated bacteria dominate the built environment (BE). Following decontamination of floors, toilet seats, and soap dispensers in four public restrooms, in situ bacterial communities were characterized hourly, daily, and weekly to determine their successional ecology. The viability of cultivable bacteria, following the removal of dispersal agents (humans), was also assessed hourly. A late-successional community developed within 5 to 8 h on restroom floors and showed remarkable stability over weeks to months. Despite late-successional dominance by skin- and outdoor-associated bacteria, the most ubiquitous organisms were predominantly gut-associated taxa, which persisted following exclusion of humans. Staphylococcus represented the majority of the cultivablemorecommunity, even after several hours of human exclusion. Methicillin-resistant Staphylococcus aureus (MRSA)-associated virulence genes were found on floors but were not present in assembled Staphylococcus pan-genomes. Viral abundances, which were predominantly enterophages, human papilloma virus, and herpesviruses, were significantly correlated with bacterial abundances and showed an unexpectedly low virus-to-bacterium ratio in surface-associated samples, suggesting that bacterial hosts are mostly dormant on BE surfaces.less

  6. Biodegradable microfluidic scaffolds for tissue engineering from amino alcohol-based poly(ester amide) elastomers

    E-Print Network [OSTI]

    Wang, Jane

    Biodegradable polymers with high mechanical strength, flexibility and optical transparency, optimal degradation properties and biocompatibility are critical to the success of tissue engineered devices and drug delivery ...

  7. Low prevalence of connexin-40 gene variants in atrial tissues and blood from atrial fibrillation subjects

    E-Print Network [OSTI]

    Tchou, Gregory D; Wirka, Robert C; Van Wagoner, David R; Barnard, John; Chung, Mina K; Smith, Jonathan D

    2012-01-01

    from atrial fibrillation subjects. BMC Medical Genetics 2012from atrial fibrillation subjects Gregory D Tchou 1 , Robertblood samples from 91 lone AF subjects and 67 atrial tissue-

  8. AcceptedPreprint Tissue Engineering and Delivery Systems Biotechnology and Bioengineering

    E-Print Network [OSTI]

    Yanikoglu, Berrin

    AcceptedPreprint 1 Tissue Engineering and Delivery Systems Biotechnology and Bioengineering DOI 10 Sciences, Sabanci University, Istanbul 34956, Turkey 2 Biological Sciences & Bioengineering, Faculty

  9. Mechanisms underlying cellular responses of cells from haemopoietic tissue to low

    SciTech Connect (OSTI)

    Kadhim, Munira A

    2012-08-22

    The above studies will provide fundamental mechanistic information relating genetic predisposition to important low dose phenomena, and will aid in the development of Department of Energy policy, as well as radiation risk policy for the public and the workplace. We believe the proposed studies accurately reflect the goals of the DOE low dose program. To accurately define the risks associated with human exposure to relevant environmental doses of low LET ionizing radiation, it is necessary to completely understand the biological effects at very low doses (i.e. less than 0.1 Gy), including the lowest possible dose, that of a single electron track traversal. At such low doses, a range of studies have shown responses in biological systems which are not related to the direct interaction of radiation tracks with DNA. The role of these "??non-targeted"? responses in critical tissues is poorly understood and little is known regarding the underlying mechanisms. Although critical for dosimetry and risk assessment, the role of individual genetic susceptibility in radiation risk is not satisfactorily defined at present. The aim of the proposed grant is to critically evaluate non-targeted effects of ionizing radiation with a focus on the induction of genomic instability (GI) in key stem cell populations from haemopoietic tissue. Using stem cells from two mouse strains (CBA/CaH and C57BL/6J) known to differ in their susceptibility to radiation effects, we plan to carefully dissect the role of genetic predisposition in these models on genomic instability. We will specifically focus on the effects of low doses of low LET radiation, down to the dose of 10mGy (0.01Gy) X-rays. Using conventional X-ray and we will be able to assess the role of genetic variation under various conditions at a range of doses down to the very low dose of 0.01Gy. Irradiations will be carried out using facilities in routine operation for such studies. Mechanistic studies of instability in different cell lineages will include the role of cytokines which have been shown to be in the initiation of instability. These studies also aim to uncover the possible mechanism of the initiation, perpetuation and delayed pathways of the instability response using relevant biological endpoints i.e. chromosomal instability, apoptosis induction, cytokine and gene array analysis. Integral to these studies will be an assessment of the role of genetic susceptibility in these responses, using CBA/CaH and C57BL/6J mice. The overall results suggest that low dose low LET X-irradiation induced delayed GI in both CBA/CaH and C57BL/6J haemopoeitic tissue. Using several biological approaches, some key strain and dose-specific differences have been identified in radiation-induced signalling in the initiation and perpetuation of the instability process. Furthermore, the induction of non-targeted radiation effects and genetic dependency may be linked to the use of alternative signalling pathways and mechanisms which have potential implications on evaluation of non-targeted effects in radiation risk assessment.

  10. Lung tissue engineering : in vitro synthesis of lung tissue from neonatal and fetal rat lung cells cultured in a three-dimensional collagen matrix

    E-Print Network [OSTI]

    Chen, Patty P., 1981-

    2004-01-01

    The focus of this study was to investigate the histology of tissue formed when fetal (16-20 days gestation) and neonatal (2 days old) rat lung cells were grown in a collagen-glycosaminoglycan scaffold. This project employed ...

  11. The uptake, tissue distribution and depuration of a polychlorinated naphthalene (Halowax 1099) in relation to tissue lipid levels in the American oyster Crassostrea virginica

    E-Print Network [OSTI]

    Rue, William James

    1979-01-01

    . In addition, one oyster death was recorded within the first two days of depuration in each of the control and 60 ppb tank-. Because of the similar results in the control and 60 ppb exposure groups it appears that Halowax 1099 does not contribute...THE UPTAKE, TISSUE DISTRIBUTION AND DEPURATION OF A POLYCHLORINATED NAPHTHALENE (HALOWAX 1099) IN RELATION TO TISSUE LIPID LEVELS IN THE AMERICAN OYSTER CRASSOSTREA VIRGINICA A Thesis by WILLIAM JAMES RUE, JR. Submitted to the Graduate...

  12. Texas Tech University Human Resources

    E-Print Network [OSTI]

    Rock, Chris

    to redevelop Texas Tech University's human resource functions into a high-performing human capital managementTexas Tech University Human Resources Strategic Plan January 1, 2014 - December 31, 2016 #12;2 Mission Texas Tech University is recognized as a premier institution and a workplace of choice. This work

  13. Understanding Human Experience Henry Kautz

    E-Print Network [OSTI]

    Kautz, Henry

    Understanding Human Experience Henry Kautz One of the earliest goals of research in artificial intelligence was to create systems that can interpret and understand day to day human experience. Early work on the goal of building systems that understand human experience. Each of the previous barriers is weakened

  14. Human Capital Management Accountability Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2008-08-01

    The Order establishes requirements, roles and responsibilities for the Human Capital Management Accountability Program (HCMAP) for human resources programs and personnel and ensures that human capital activities are regulatory and procedurally compliant with Federal statutes and Departmental policies. Does not cancel other directives.

  15. Contrasting signals of positive selection in genes involved in human skin color variation from tests based on SNP scans and resequencing

    E-Print Network [OSTI]

    de Gruijter, Johanna Maria; Lao, Oscar; Vermeulen, Mark; Xue, Yali; Woodwark, Cara; Gillson, Christopher J.; Coffey, Alison J.; Ayub, Qasim; Mehdi, S. Qasim; Kayser, Manfred; Tyler-Smith, Chris

    2011-12-01

    Eu NeEA NeA NeEuA Present TsplitEurasia TsplitOOA Figure 2 Out-of-Africa (OOA) model implemented in the forward simulator and further used for approximate Bayesian computation (ABC) estimation. An ancestral population with size NeA splits at Tsplit... OOA into two new populations: Africa (with NeAf) and Eurasia (with NeEuA), and this population at TsplitEurasia splits in two populations, Europe (with NeEu) and Asia (with NeEA). Table 3 Median and dispersion statistics of the posterior distributions...

  16. Probing embryonic tissue mechanics with laser hole-drilling

    E-Print Network [OSTI]

    Ma, Xiaoyan; Scully, Peter C; Hutson, M Shane

    2008-01-01

    We have used laser hole-drilling to assess mechanical changes in an embryonic epithelium during development - in vivo and with subcellular resolution. This method uses a single laser pulse to ablate a cylindrical hole (1 micron in diameter, 5-7 micron tall) clean through the epithelium, and tracks the subsequent recoil of adjacent cells (with ms time resolution). We investigate dorsal closure in the fruit fly with emphasis on apical constriction of amnioserosa cells. We find that substantial in-plane tension is carried across each cell surface and not just along cell-cell interfaces. In early phases of constriction, the tension is 1.6-fold greater along cell-cell interfaces. Later, the two tensions are indistinguishable. Other changes associated with constriction include a decrease in the characteristic recoil times and an increased anisotropy. The smaller time constants imply a more solid-like tissue. The anisotropy matches changes in the underlying quasi-hexagonal cellular mesh. The results of these laser h...

  17. Light ion irradiation for unfavorable soft tissue sarcoma

    SciTech Connect (OSTI)

    Linstadt, D.; Castro, J.R.; Phillips, T.L.; Petti, P.L.; Collier, J.M.; Daftari, I.; Schoethaler, R.; Rayner, A.

    1990-09-01

    Between 1978 and 1989, 32 patients with unfavorable soft tissue sarcoma underwent light ion (helium, neon) irradiation with curative intent at Lawrence Berkeley Laboratory. The tumors were located in the trunk in 22 patients and head and neck in 10. Macroscopic tumor was present in 22 at the time of irradiation. Two patients had tumors apparently induced by previous therapeutic irradiation. Follow-up times for surviving patients ranged from 4 to 121 months (median 27 months). The overall 3-year actuarial local control rate was 62%; the corresponding survival rate was 50%. The 3-year actuarial control rate for patients irradiated with macroscopic tumors was 48%, while none of the patients with microscopic disease developed local recurrence (100%). The corresponding 3-year actuarial survival rates were 40% (macroscopic) and 78% (microscopic). Patients with retroperitoneal sarcoma did notably well; the local control rate and survival rate were 64% and 62%, respectively. Complications were acceptable; there were no radiation related deaths, while two patients (6%) required operations to correct significant radiation-related injuries. These results appear promising compared to those achieved by low -LET irradiation, and suggest that this technique merits further investigation.

  18. Sequential Causal Learning in Humans and Rats

    E-Print Network [OSTI]

    Lu, Hongjing; Rojas, Randall R.; Beckers, Tom; Yuille, Alan

    2008-01-01

    selection, to a human experiment that employed pretraining (group (white) in human experiment by Beckers et al. (2005).set used for the human experiments, we increased the

  19. Sequential Causal Learning in Humans and Rats

    E-Print Network [OSTI]

    Hongjing Lu; Randall R. Rojas; Tom Beckers; Alan Yuille

    2011-01-01

    selection, to a human experiment that employed pretraining (group (white) in human experiment by Beckers et al. (2005).set used for the human experiments, we increased the

  20. Allele-specific gene regulation in humans

    E-Print Network [OSTI]

    Maynard, Nathaniel David

    2008-01-01

    1 Introduction The Human Genome Project has provided thefrom clones and the human genome project have revealed thatVariation The Human Genome Project provided scientists with

  1. Project ATHENA creates surrogate human organ systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Project ATHENA creates surrogate human organ systems Project ATHENA creates surrogate human organ systems The development of miniature surrogate human organs, coupled with highly...

  2. Reply: Lithium and Increased Cortical Gray Matter--More Tissue or More Water?

    E-Print Network [OSTI]

    Thompson, Paul

    Reply: Lithium and Increased Cortical Gray Matter--More Tissue or More Water? To the Editor: W e. Regenold voices some disappointment that we did not determine whether an increase in brain tissue water patients. Although lithium's effects on body water homeostasis (2) are important to consider, the absence

  3. On constitutive modeling of soft tissue for the long-term prediction of cranio-maxillofacial

    E-Print Network [OSTI]

    Frey, Pascal

    -incompressible, nonlinear plasticviscoelastic material properties. Besides the properties well known from classic material in cranio-maxillofacial (CMF) surgery simulations. Since the biomechanical properties of living tissues and comprehensive data on material properties of a particular tissue are still very scarce. Thus, simplified models

  4. RADIATION HEAT TRANSFER IN TISSUE WELDING AND SOLDERING WITH ULTRAFAST LASERS

    E-Print Network [OSTI]

    Guo, Zhixiong "James"

    RADIATION HEAT TRANSFER IN TISSUE WELDING AND SOLDERING WITH ULTRAFAST LASERS Kyunghan Kim to incorporate transient radiation heat transfer in tissue welding and soldering with use of ultrafast lasers are performed between laser welding and laser soldering. The use of solder is found to substantially enhance

  5. Paper-supported 3D cell culture for tissue-based bioassays

    E-Print Network [OSTI]

    Prentiss, Mara

    , generated, and tested (6). Chemical gradients in tissues are often generated by cellular metabolism. Hence-sensitive genes are overex- pressed. Altering gas permeability at the ends of stacks controlled the gradient molecular gradients and to mimic tissue- and organ-level functions. diffusion hypoxia molecular gradients

  6. Mechanical interactions between collagen and proteoglycans: implications for the stability of lung tissue

    E-Print Network [OSTI]

    Alencar, Adriano Mesquita

    Mechanical interactions between collagen and proteoglycans: implications for the stability of lung- tions for the stability of lung tissue. J Appl Physiol 98: 672679, 2005. First published September 24- ticity of the connective tissue including lung parenchyma. The gly- cosaminoglycans on the proteoglycans

  7. Expression quantitative trait loci detected in cell lines are often present in primary tissues

    E-Print Network [OSTI]

    Coop, Graham

    conducted in lympho- blastoid cell lines (LCLs), rather than primary tissues, mostly by using the Hap assays in LCLs may often be the only feasible approach (e.g. 16) to further study the potential function the overlap of eQTLs found in LCLs and primary tissues. For one, differences in study designs, platforms

  8. Experimental Setup Measurements were made with the experimental configuration depicted in Figure 1. Tissue

    E-Print Network [OSTI]

    Arthur, R. Martin

    water, which had been degassed by vacuum pumping in an appropriate vessel. Tissue was placed reflected from sub-wavelength scatterers. Statistical analysis of the distribution of change be used to infer the temperature of the tissue by analyzing the statistics of the collection. We found

  9. A Framework for Unsupervised Segmentation of Lung Tissues from Low Dose Computed

    E-Print Network [OSTI]

    Kim, Tae-Kyun

    A Framework for Unsupervised Segmentation of Lung Tissues from Low Dose Computed Tomography Images of lung tis- sues from Low Dose Computed Tomography (LDCT) are proposed. In this paper we describe LDCT images and desired maps of regions (lung and the other chest tissues) by a joint Markov-Gibbs random

  10. Rapid Communication On the origin of the toughness of mineralized tissue

    E-Print Network [OSTI]

    Ritchie, Robert

    Rapid Communication On the origin of the toughness of mineralized tissue: microcracking or crack in mineralized tissues, such as bone and dentin, have been identified--microcracking and crack bridging. While the perspective of developing a micromechanistic framework for understanding fracture behavior of mineralized

  11. Tissue heterogeneity in the mouse lung: effects of elastase treatment Satoru Ito,1

    E-Print Network [OSTI]

    Lutchen, Kenneth

    Tissue heterogeneity in the mouse lung: effects of elastase treatment Satoru Ito,1 Edward P heterogeneity in the mouse lung: effects of elastase treatment. J Appl Physiol 97: 204212, 2004. First to characterize heterogeneity of tissue elasticity of the lung. The model includes a parallel set of pathways

  12. Engineered Heart Tissue Model of Diabetic Myocardium Hannah Song, Ph.D.,1

    E-Print Network [OSTI]

    Zandstra, Peter W.

    Engineered Heart Tissue Model of Diabetic Myocardium Hannah Song, Ph.D.,1 Peter W. Zandstra, Ph of cardiomyocytes (CMs) is a leading cause of heart failure. Previously, we reported an in vitro test-bed for screening cell integration between injected test cells and host CM using the engineered heart tissue

  13. Engineered Heart Tissue Enables Study of Residual Undifferentiated Embryonic Stem Cell Activity in a

    E-Print Network [OSTI]

    Zandstra, Peter W.

    ARTICLE Engineered Heart Tissue Enables Study of Residual Undifferentiated Embryonic Stem Cell, Canada, M5S 3G9 6 Heart and Stroke/Richard Lewar Centre of Excellence, Toronto, Ontario, Canada, M5S 3G9 cell survival. As an alternative, we have used an engineered heart tissue (EHT) based on neonatal rat

  14. Optical Histology: A Method to Visualize Microvasculature in Thick Tissue Sections of Mouse Brain

    E-Print Network [OSTI]

    Rose, Michael R.

    Optical Histology: A Method to Visualize Microvasculature in Thick Tissue Sections of Mouse Brain% paraformaldehyde. The organ is then sliced into 1 mm sections and optically cleared, or made transparent, using FocusClear, a proprietary optical clearing agent. After optical clearing, the DiI-labeled tissue

  15. Scanning electron microscope study of connective tissue in raw and cooked muscles

    E-Print Network [OSTI]

    Percy, Mary Lou

    1976-01-01

    . D. E. , Hoekstra, W, G. and Bray, R. W. 1964. Age associated changes in bovine muscle connective tissue. II. Exposure to increasing temperature. J. Food Sci. 29:615. Ham, A. M. 1965. Muscular tissue. In "Histology, " ed. Ham, A. W. p. 476. J. B...

  16. STRESS-INDUCED SPINE LOSS IN THE MEDIAL AMYGDALA IS MEDIATED BY TISSUE-PLASMINOGEN ACTIVATOR

    E-Print Network [OSTI]

    STRESS-INDUCED SPINE LOSS IN THE MEDIAL AMYGDALA IS MEDIATED BY TISSUE-PLASMINOGEN ACTIVATOR S Avenue, New York, NY 10021, USA Abstract--The amygdala, which exerts a regulatory influence on the stress response, is itself affected by stress. It has been reported that the serine protease tissue

  17. Choice of reconstructed tissue properties affects interpretation of lung EIT images

    E-Print Network [OSTI]

    Adler, Andy

    Choice of reconstructed tissue properties affects interpretation of lung EIT images Bartlomiej patients. In lung EIT, the EIT inverse problem is commonly linearised and only changes in electrical properties of the lung and artefacts introduced by the linearisation. #12;Choice of reconstructed tissue

  18. SUPPLEMENTARY METHODS AND TABLES Drug transport from the blood into the lung tissue

    E-Print Network [OSTI]

    Kirschner, Denise

    1 SUPPLEMENTARY METHODS AND TABLES Drug transport from the blood into the lung tissue To study that enters the grid representing lung parenchyma via vascular sources, and diffuses among micro Once TNF inhibitors penetrate from blood into the lung tissue, they can bind TNF and thus block TNF

  19. ROBUST SEGMENTATION OF LUNG TISSUE IN CHEST CT SCANNING Amal Farag, James Graham and Aly Farag

    E-Print Network [OSTI]

    Louisville, University of

    ROBUST SEGMENTATION OF LUNG TISSUE IN CHEST CT SCANNING Amal Farag, James Graham and Aly Farag.edu ABSTRACT This paper deals with segmentation of the lung tissues from low dose CT (LDCT) scans of the chest. Goal is correct segmentation as well as maintaining the details of the lung region in the chest cavity

  20. Fresnel Effect in Radiation Transfer in Biological Tissues Kyunghan Kim and Zhixiong Guo*

    E-Print Network [OSTI]

    Guo, Zhixiong "James"

    Fresnel Effect in Radiation Transfer in Biological Tissues Kyunghan Kim and Zhixiong Guo* MAE Method (DOM) to incorporate Fresnel's boundary in laser radiation transport in biological tissues is calculated by the use of Snell's law and Fresnel's equation. The radiation fields, including the radiative