National Library of Energy BETA

Sample records for human cortical bone

  1. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Irradiation Effects on Human Cortical Bone Fracture Behavior Print Human bone is strong but still fallible. To better predict fracturing in bone, researchers need a mechanistic...

  2. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Source Beamline 8.3.2 to investigate changes in crack path and toughening mechanisms in human cortical bone with increased exposure to radiation, finding that exposure to high...

  3. Mixed-mode fracture of human cortical bone Elizabeth A. Zimmermann a,b

    E-Print Network [OSTI]

    Ritchie, Robert

    Mixed-mode fracture of human cortical bone Elizabeth A. Zimmermann a,b , Maximilien E. Launey Available online 1 July 2009 Keywords: Human cortical bone Mixed-mode fracture Fracture toughness Fracture mechanisms a b s t r a c t Although the mode I (tensile opening) fracture toughness has been the focus

  4. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fracture Behavior Print Human bone is strong but still fallible. To better predict fracturing in bone, researchers need a mechanistic framework to understand the changes taking...

  5. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    28 July 2010 00:00 Human bone is strong but still fallible. To better predict fracturing in bone, researchers need a mechanistic framework to understand the changes taking...

  6. Fracture resistance of human cortical bone across multiple length-scales at physiological strain rates

    E-Print Network [OSTI]

    Ritchie, Robert

    Fracture resistance of human cortical bone across multiple length-scales at physiological strain Accepted 22 March 2014 Available online 13 April 2014 Keywords: Bone Strain rate Fracture toughness Plasticity X-ray diffraction a b s t r a c t While most fracture-mechanics investigations on bone have been

  7. Effect of aging on the toughness of human cortical bone: evaluation by R-curves

    E-Print Network [OSTI]

    Ritchie, Robert

    Effect of aging on the toughness of human cortical bone: evaluation by R-curves R.K. Nallaa,b , J online 27 October 2004 Abstract Age-related deterioration of the fracture properties of bone, coupled, and hence, an understanding of how its fracture properties degrade with age is essential. The present study

  8. Biomaterials 27 (2006) 20952113 Fracture length scales in human cortical bone

    E-Print Network [OSTI]

    Ritchie, Robert

    2006-01-01

    Biomaterials 27 (2006) 2095­2113 Fracture length scales in human cortical bone: The necessity of nonlinear fracture models Q.D. Yanga,Ã, Brian N. Coxa , Ravi K. Nallab , R.O. Ritchieb a Rockwell Scientific; accepted 26 September 2005 Available online 4 November 2005 Abstract Recently published data for fracture

  9. The significance of crack-resistance curves to the mixed-mode fracture toughness of human cortical bone

    E-Print Network [OSTI]

    Ritchie, Robert

    The significance of crack-resistance curves to the mixed-mode fracture toughness of human cortical cortical bone Mixed-mode fracture Fracture toughness Crack-growth resistance curve a b s t r a c t The majority of fracture mechanics studies on the toughness of bone have been performed under tensile loading

  10. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    on different size scales within bone, as well as the role of sustained irradiation damage. Combining in situ mechanical testing with synchrotron x-ray diffraction imaging and...

  11. Characterization of the effects of x-ray irradiation on the hierarchical structure and mechanical properties of human cortical bone

    E-Print Network [OSTI]

    Ritchie, Robert

    each hier- archical structural level contributes to its strength, ductility and toughness-ray exposures up to 630 kGy. Macroscopically, bone strength, ductility and fracture resistance are seen August 2011 Keywords: Human cortical bone Deformation Toughness X-ray diffraction Tomography Collagen a b

  12. Individual-specific multi-scale finite element simulation of cortical bone of human proximal femur

    SciTech Connect (OSTI)

    Ascenzi, Maria-Grazia, E-mail: mgascenzi@mednet.ucla.edu [UCLA/Orthopaedic Hospital, Department of Orthopaedic Surgery, Rehabilitation Bldg, Room 22-69, 1000 Veteran Avenue, University of California, Los Angeles, CA 90095 (United States)] [UCLA/Orthopaedic Hospital, Department of Orthopaedic Surgery, Rehabilitation Bldg, Room 22-69, 1000 Veteran Avenue, University of California, Los Angeles, CA 90095 (United States); Kawas, Neal P., E-mail: nealkawas@ucla.edu [UCLA/Orthopaedic Hospital, Department of Orthopaedic Surgery, Rehabilitation Bldg, Room 22-69, 1000 Veteran Avenue, University of California, Los Angeles, CA 90095 (United States); Lutz, Andre, E-mail: andre.lutz@hotmail.de [Institute of Biomechanics and Numerical Mechanics, Leibniz University Hannover, 30167 Hannover (Germany)] [Institute of Biomechanics and Numerical Mechanics, Leibniz University Hannover, 30167 Hannover (Germany); Kardas, Dieter, E-mail: kardas@ibnm.uni-hannover.de [ContiTech Vibration Control, Jaedekamp 30 None, 30419 Hannover (Germany)] [ContiTech Vibration Control, Jaedekamp 30 None, 30419 Hannover (Germany); Nackenhorst, Udo, E-mail: nackenhorst@ibnm.uni-hannover.de [Institute of Biomechanics and Numerical Mechanics, Leibniz University Hannover, 30167 Hannover (Germany)] [Institute of Biomechanics and Numerical Mechanics, Leibniz University Hannover, 30167 Hannover (Germany); Keyak, Joyce H., E-mail: jhkeyak@uci.edu [Department of Radiological Sciences, Medical Sciences I, Bldg 811, Room B140, University of California, Irvine, CA 92697-5000 (United States)

    2013-07-01

    We present an innovative method to perform multi-scale finite element analyses of the cortical component of the femur using the individual’s (1) computed tomography scan; and (2) a bone specimen obtained in conjunction with orthopedic surgery. The method enables study of micro-structural characteristics regulating strains and stresses under physiological loading conditions. The analysis of the micro-structural scenarios that cause variation of strain and stress is the first step in understanding the elevated strains and stresses in bone tissue, which are indicative of higher likelihood of micro-crack formation in bone, implicated in consequent remodeling or macroscopic bone fracture. Evidence that micro-structure varies with clinical history and contributes in significant, but poorly understood, ways to bone function, motivates the method’s development, as does need for software tools to investigate relationships between macroscopic loading and micro-structure. Three applications – varying region of interest, bone mineral density, and orientation of collagen type I, illustrate the method. We show, in comparison between physiological loading and simple compression of a patient’s femur, that strains computed at the multi-scale model’s micro-level: (i) differ; and (ii) depend on local collagen-apatite orientation and degree of calcification. Our findings confirm the strain concentration role of osteocyte lacunae, important for mechano-transduction. We hypothesize occurrence of micro-crack formation, leading either to remodeling or macroscopic fracture, when the computed strains exceed the elastic range observed in micro-structural testing.

  13. Age-related changes in the plasticity and toughness of human cortical bone at multiple length-scales

    SciTech Connect (OSTI)

    Zimmermann, Elizabeth A.; Schaible, Eric; Bale, Hrishikesh; Barth, Holly D.; Tang, Simon Y.; Reichert, Peter; Busse, Bjoern; Alliston, Tamara; Ager III, Joel W.; Ritchie, Robert O.

    2011-08-10

    The structure of human cortical bone evolves over multiple length-scales from its basic constituents of collagen and hydroxyapatite at the nanoscale to osteonal structures at nearmillimeter dimensions, which all provide the basis for its mechanical properties. To resist fracture, bone’s toughness is derived intrinsically through plasticity (e.g., fibrillar sliding) at structural-scales typically below a micron and extrinsically (i.e., during crack growth) through mechanisms (e.g., crack deflection/bridging) generated at larger structural-scales. Biological factors such as aging lead to a markedly increased fracture risk, which is often associated with an age-related loss in bone mass (bone quantity). However, we find that age-related structural changes can significantly degrade the fracture resistance (bone quality) over multiple lengthscales. Using in situ small-/wide-angle x-ray scattering/diffraction to characterize sub-micron structural changes and synchrotron x-ray computed tomography and in situ fracture-toughness measurements in the scanning electron microscope to characterize effects at micron-scales, we show how these age-related structural changes at differing size-scales degrade both the intrinsic and extrinsic toughness of bone. Specifically, we attribute the loss in toughness to increased non-enzymatic collagen cross-linking which suppresses plasticity at nanoscale dimensions and to an increased osteonal density which limits the potency of crack-bridging mechanisms at micron-scales. The link between these processes is that the increased stiffness of the cross-linked collagen requires energy to be absorbed by “plastic” deformation at higher structural levels, which occurs by the process of microcracking.

  14. Characterization of the effects of x-ray irradiation on the hierarchical structure and mechanical properties of human cortical bone

    SciTech Connect (OSTI)

    Barth, Holly; Zimmermann, Elizabeth; Schaible, Eric; Tang, Simon; Alliston, Tamara; Ritchie, Robert

    2011-08-19

    Bone comprises a complex structure of primarily collagen, hydroxyapatite and water, where each hierarchical structural level contributes to its strength, ductility and toughness. These properties, however, are degraded by irradiation, arising from medical therapy or bone-allograft sterilization. We provide here a mechanistic framework for how irradiation affects the nature and properties of human cortical bone over a range of characteristic (nano to macro) length-scales, following x-­ray exposures up to 630 kGy. Macroscopically, bone strength, ductility and fracture resistance are seen to be progressively degraded with increasing irradiation levels. At the micron-­scale, fracture properties, evaluated using in-situ scanning electron microscopy and synchrotron x-ray computed micro-tomography, provide mechanistic information on how cracks interact with the bone-matrix structure. At sub-micron scales, strength properties are evaluated with in-situ tensile tests in the synchrotron using small-/wide-angle x-ray scattering/diffraction, where strains are simultaneously measured in the macroscopic tissue, collagen fibrils and mineral. Compared to healthy bone, results show that the fibrillar strain is decreased by ~40% following 70 kGy exposures, consistent with significant stiffening and degradation of the collagen. We attribute the irradiation-­induced deterioration in mechanical properties to mechanisms at multiple length-scales, including changes in crack paths at micron-­scales, loss of plasticity from suppressed fibrillar sliding at sub-­micron scales, and the loss and damage of collagen at the nano-­scales, the latter being assessed using Raman and Fourier-Transform-Infrared spectroscopy and a fluorometric assay.

  15. On the effect of x-ray irradiation on the deformation and fracture behavior of human cortical bone

    E-Print Network [OSTI]

    Barth, Holly D.

    2010-01-01

    effects  of  gamma  irradiation  on  allograft  biology S.   Effects  of  gamma? irradiation  on  the  human bone  after  gamma  irradiation.   J.   Bone  Joint  Surg. ?

  16. Characterization of the effects of x-ray irradiation on the hierarchical structure and mechanical properties of human cortical bone

    E-Print Network [OSTI]

    Barth, Holly

    2012-01-01

    severe  loss  in  the  plastic  properties  of  bone  with  the  plastic,  rather  than  elastic,  properties  of  

  17. A comparative study of young and mature bovine cortical bone q Zherrina Manilay a

    E-Print Network [OSTI]

    McKittrick, Joanna

    .% proteins (mostly collagen-I, with a small amount of non-collageneous proteins) and 10 wt.% water [1 is present in the areas that need to absorb energy (skull, ribs, vertebra). Cortical bone further consists growing animals and is an indicator of non-human bone [4]. Katz and Yoon [5] showed that plexiform areas

  18. Author's personal copy Initial anisotropy in demineralized bovine cortical bone in compressive

    E-Print Network [OSTI]

    McKittrick, Joanna

    Author's personal copy Initial anisotropy in demineralized bovine cortical bone in compressive Cortical bone Cyclic compression Demineralization The mechanical properties of demineralized bovine and transverse directions. The bone was demineralized by 10% ethylenediaminetetraacetic acid (EDTA) (100% protein

  19. Elastic moduli of untreated, demineralized and deproteinized cortical bone: Validation of a theoretical model of bone as an interpenetrating composite material

    E-Print Network [OSTI]

    McKittrick, Joanna

    Elastic moduli of untreated, demineralized and deproteinized cortical bone: Validation online 15 November 2011 Keywords: Cortical bone Elastic moduli Multi-scale modeling Demineralization include completely demineralized and deproteinized bones as well as untreated bone samples. Porosity

  20. Anisotropy in the compressive mechanical properties of bovine cortical bone and the mineral and protein constituents

    E-Print Network [OSTI]

    McKittrick, Joanna

    Cortical bone Compression Demineralization Deproteinization a b s t r a c t The mechanical properties of fully demineralized, fully deproteinized and untreated cortical bovine femur bone were investigated. Demineralization and deproteinization of the bone demonstrated that contiguous, stand-alone structures result

  1. Independent measurement of femoral cortical thickness and cortical bone density using clinical CT

    E-Print Network [OSTI]

    Treece, G. M.; Gee, A. H.

    2014-12-05

    for CBM v2 in eq. (4) by using eq. (1) to simulate the CT data variation c(x) through ideal cortices, i.e. with perfectly constant density and no pores, surrounded by material which is also at a constant (but lower) density. These cortices were blurred... are shown in Fig. 4. 3.2. Comparing HRpQCT and QCT data Moving on from simulations, we examined data from a study ethically approved by the Medical University of 5 0 1 2 3 4 5 0 500 1000 1500 thickness (mm) de ns ity (H U) yb y m raw data model fit...

  2. Cortical Projection Topography of the Human Splenium: Hemispheric Asymmetry and Individual Differences

    E-Print Network [OSTI]

    Gazzaniga, Michael

    Cortical Projection Topography of the Human Splenium: Hemispheric Asymmetry and Individual topography of the human splenium. Homotopic and heterotopic connections were revealed between the splenium difficult to trace the cortical projection topographies of long white matter fiber tracts of the human brain

  3. Predicting hip fracture type with cortical bone mapping (CBM) in the Osteoporotic Fractures in Men (MrOS) study

    E-Print Network [OSTI]

    Drummond, Tom

    Predicting hip fracture type with cortical bone mapping (CBM) in the Osteoporotic Fractures in Men, for the Osteoporotic Fractures in Men (MrOS) study CUED/F-INFENG/TR 695 January 2015 Cambridge University Engineering;1 Abstract Hip fracture risk is known to be related to material properties of the proximal femur, but prospec

  4. On the multiscale origins of fracture resistance in human bone and its biological degradation

    SciTech Connect (OSTI)

    Zimmermann, Elizabeth A.; Barth, Holly D.; Ritchie, Robert O.

    2012-03-09

    Akin to other mineralized tissues, human cortical bone can resist deformation and fracture due to the nature of its hierarchical structure, which spans the molecular to macroscopic length-scales. Deformation at the smallest scales, mainly through the composite action of the mineral and collagen, contributes to bone?s strength or intrinsic fracture resistance, while crack-tip shielding mechanisms active on the microstructural scale contribute to the extrinsic fracture resistance once cracking begins. The efficiency with which these structural features can resist fracture at both small and large length-scales becomes severely degraded with such factors as aging, irradiation and disease. Indeed aging and irradiation can cause changes to the cross-link profile at fibrillar length-scales as well as changes at the three orders of magnitude larger scale of the osteonal structures, both of which combine to inhibit the bone's overall resistance to the initiation and growth of cracks.

  5. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    dose. The graphs show that there is a severe and progressive degradation in mechanical properties, specifically in the bending stressstrain properties, with increase in x-ray...

  6. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATIONIntroducing theActivation by Cytochrome P450 |

  7. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATIONIntroducing theActivation by Cytochrome P450 |Irradiation

  8. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATIONIntroducing theActivation by Cytochrome P450

  9. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATIONIntroducing theActivation by Cytochrome P450Irradiation

  10. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATIONIntroducing theActivation by Cytochrome

  11. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenryInhibitingInteractivePGAS andUniversity IonIron is the Key

  12. Irradiation Effects on Human Cortical Bone Fracture Behavior

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenryInhibitingInteractivePGAS andUniversity IonIron is the

  13. Correlation of mechanical viscoelastic properties to microstructure of equine cortical bone tissue 

    E-Print Network [OSTI]

    Ayers, Andrew Kerr

    1995-01-01

    were taken from equine third metacarpal bones and machined into rectangular slabs approximately 46 mm long, 10 mm wide, and 2 mm thick. After initial testing to determine the linear viscoelastic region of equine compact bone, the specimens were tested...

  14. Methods for testing the strength of cancellous bone and tested method effects on cortical bone in the ovariectomized rat 

    E-Print Network [OSTI]

    Ruhmann, Sean Phillip

    1998-01-01

    In this study, two mechanical testing procedures were developed to test the strength of cancellous bone from the proximal tibia of the rat, the "punch method" and the "whole slice method". These were used to quantify the effect of ovariectomy on rat...

  15. Fracture, aging and disease in bone

    E-Print Network [OSTI]

    Ager, J.W.; Balooch, G.; Ritchie, R.O.

    2006-01-01

    and R. O. Ritchie: Effect of aging on the toughness of humanof microstructure in the aging-related deterioration of thestudy of the effect of aging on human cortical bone J.

  16. Human Motor Cortical Activity Is Selectively Phase-Entrained on Underlying Rhythms

    E-Print Network [OSTI]

    Knight, Robert T.

    Human Motor Cortical Activity Is Selectively Phase- Entrained on Underlying Rhythms Kai J. Miller1 that the amplitude of this broadband motif is entrained on the phase of the beta rhythm, as well as rhythms at other frequencies, in peri-central cortex during fixation. During finger movement, the beta phase-entrainment

  17. Effect of cryo-induced microcracks on microindentation of hydrated cortical bone tissue

    E-Print Network [OSTI]

    Qin, Qinghua

    tissue was plastic deformation, not brittle fracture. © 2009 Elsevier Inc. All rights reserved. Keywords 1. Introduction Bone contains approximately 60% ceramic nanoparticles of inorganic carbonated hydroxyapatite, 10% water and about 30% polymer matrix of organic and mineralized collagen fibers by weight [1

  18. Structural Analysis of Human and Bovine Bone for Development of Synthetic Materials 

    E-Print Network [OSTI]

    Jang, Eunhwa

    2012-10-19

    With increasing demands in bone repair and replacement, this research investigates the microstructure, properties and performance of bovine bone, human bone, and synthetic materials. Doing so, experimental approaches were used to exam and compare...

  19. Generation of human cortical neurons from a new immortal fetal neural stem cell line

    SciTech Connect (OSTI)

    Cacci, E. [Laboratory of Neural Stem Cell Biology, Section of Restorative Neurology, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, BMC B10, Klinikgatan 26, University Hospital, SE-221 84 Lund (Sweden); Villa, A. [Laboratory of Human Neural Stem Cell Research, Center of Molecular Biology Severo Ochoa, Lab CX-450, Autonomous University of Madrid, 28049 Madrid (Spain); Parmar, M. [Division of Neurobiology, Wallenberg Neuroscience Center, Lund University, BMC A11, SE-221 84 Lund (Sweden); Cavallaro, M. [Laboratory of Neural Stem Cell Biology, Section of Restorative Neurology, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, BMC B10, Klinikgatan 26, University Hospital, SE-221 84 Lund (Sweden); Mandahl, N. [Department of Laboratory Medicine, Section of Clinical Genetics, University Hospital, SE-221 85 Lund (Sweden); Lindvall, O. [Laboratory of Neurogenesis and Cell Therapy, Section of Restorative Neurology, Wallenberg Neuroscience Center, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, University Hospital, SE-221 84 Lund (Sweden); Martinez-Serrano, A. [Laboratory of Human Neural Stem Cell Research, Center of Molecular Biology Severo Ochoa, Lab CX-450, Autonomous University of Madrid, 28049 Madrid (Spain); Kokaia, Z. [Laboratory of Neural Stem Cell Biology, Section of Restorative Neurology, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, BMC B10, Klinikgatan 26, University Hospital, SE-221 84 Lund (Sweden)]. E-mail: Zaal.Kokaia@med.lu.se

    2007-02-01

    Isolation and expansion of neural stem cells (NSCs) of human origin are crucial for successful development of cell therapy approaches in neurodegenerative diseases. Different epigenetic and genetic immortalization strategies have been established for long-term maintenance and expansion of these cells in vitro. Here we report the generation of a new, clonal NSC (hc-NSC) line, derived from human fetal cortical tissue, based on v-myc immortalization. Using immunocytochemistry, we show that these cells retain the characteristics of NSCs after more than 50 passages. Under proliferation conditions, when supplemented with epidermal and basic fibroblast growth factors, the hc-NSCs expressed neural stem/progenitor cell markers like nestin, vimentin and Sox2. When growth factors were withdrawn, proliferation and expression of v-myc and telomerase were dramatically reduced, and the hc-NSCs differentiated into glia and neurons (mostly glutamatergic and GABAergic, as well as tyrosine hydroxylase-positive, presumably dopaminergic neurons). RT-PCR analysis showed that the hc-NSCs retained expression of Pax6, Emx2 and Neurogenin2, which are genes associated with regionalization and cell commitment in cortical precursors during brain development. Our data indicate that this hc-NSC line could be useful for exploring the potential of human NSCs to replace dead or damaged cortical cells in animal models of acute and chronic neurodegenerative diseases. Taking advantage of its clonality and homogeneity, this cell line will also be a valuable experimental tool to study the regulatory role of intrinsic and extrinsic factors in human NSC biology.

  20. Preliminary sex differences in human cortical BOLD fMRI activity during the preparation of increasingly complex

    E-Print Network [OSTI]

    Sergio, Lauren E.

    Preliminary sex differences in human cortical BOLD fMRI activity during the preparation imaging (fMRI) to show significant sex-related differences in human brain activity during visual-to-motor transformation tasks. Although the behavioural performance of the male and female groups did not differ, sex

  1. High-Speed Photography of Human Trabecular Bone during Compression Philipp J. Thurner1

    E-Print Network [OSTI]

    Hansma, Paul

    column, where it fills all of the inner vertebral space. In the long bones it transfers loads from joint study, we investigated healthy, osteoarthritic, and osteoporotic human vertebral trabecular bone

  2. Three-dimensional terahertz computed tomography of human bones

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    spectroscopy cannot rival with dual energy x-ray absorptiometry (DEXA) to identify the bone mineral density

  3. Predicting hip fracture type with cortical bone mapping (CBM) in the Osteoporotic Fractures in Men (MrOS) study

    E-Print Network [OSTI]

    Treece, Graham M.; Gee, Andrew H.; Tonkin, Carol; Ewing, Susan K.; Cawthon, Peggy M.; Black, Dennis M.; Poole, Kenneth E. S.; Osteoporotic Fractures in Men Study

    2015-03-18

    for hazard ratios were conducted in SAS version 9.1 (SAS Institute, Cary, NC, USA). In addition, we examined the ability of models involving different groups of variables to predict ten-year fracture incidence, by performing either binomial (any fracture... CD, Delmas P, et al. Predictive value of BMD for hip and other fractures. J Bone Miner Res. 2005;20(7):1185–1194. 3. Kanis JA, Burlet N, Cooper C, Delmas PD, Reginster JY, Borgstrom F, et al. European guidance for the diagnosis and management...

  4. The petrous portion of the human temporal bone: potential for forensic individuation 

    E-Print Network [OSTI]

    Wiersema, Jason Matthew

    2009-06-02

    are threefold: (1) To investigate variability in the shape of the petrous portion of the human temporal bone using two-dimensional morphometric analysis; (2) to evaluate the reliability of the resultant method in forensic identification; and (3) to consider...

  5. Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin

    SciTech Connect (OSTI)

    Reisner, Y.; Kapoor, N.; Hodes, M.Z.; O'Reilly, R.J.; Good, R.A.

    1982-02-01

    Mouse bone marrow and spleen cells agglutinated by soybean agglutinin (SBA) or peanut agglutinin (PNA) were previously shown to be enriched for spleen colony-forming cells (CFU-S) and sufficiently depleted of graft-versus-host reaction producing cells to allow hematologic reconstitution of lethally irradiated allogeneic recipient mice. A similar enrichment for cells capable of forming colonies in soft agar culture (CFU-C) has now been found in the SBA-agglutinated fraction of mouse bone marrow cells, in contrast to the finding that in human bone marrow the majority of the CFU-C are in the fraction not agglutinated by SBA. Cytofluorometric studies with fluorescein-labeled SBA (FITC-SBA) revealed that the majority of both mouse and human bone marrow cells bind the lectin. Experiments mixing the human marrow fractions separated by SBA reveal that true enrichment for CFU-C is achieved in the unagglutinated fraction, as opposed to a possible depletion of a suppressor cell population. Granulocytic, monocytic, and mixed cell colonies were all enriched in the SBA-unagglutinated cell fraction from human bone marrow.

  6. HEMATOPOIESIS Soluble factor cross-talk between human bone marrow-derived hematopoietic and

    E-Print Network [OSTI]

    Zandstra, Peter W.

    HEMATOPOIESIS Soluble factor cross-talk between human bone marrow-derived hematopoietic BM-derived progenitors and the balance between these compartments. Factorial experiments demonstrated45 ) and nonhematopoietic (CD45 ) cells, as well as their derivatives. Kinetic tracking of IL-3

  7. DESIGN OF FOAM COVERING FOR ROBOTIC ARMS TO ENSURE HUMAN SAFETY Lingqi Zeng and Gary M. Bone

    E-Print Network [OSTI]

    Bone, Gary

    , and robot and human velocities. The impact experiments are performed with an apparatus simulating the humanDESIGN OF FOAM COVERING FOR ROBOTIC ARMS TO ENSURE HUMAN SAFETY Lingqi Zeng and Gary M. Bone@mcmaster.ca ABSTRACT Unintentional physical human-robot contact is becoming more common as robots operate in closer

  8. Nanomechanics and ultrastructural studies of cortical bone : fundamental insights regarding structure-function, mineral-organic force mechanics interactions, and heterogeneity

    E-Print Network [OSTI]

    Tai, Kuangshin

    2007-01-01

    Although the mechanics of bone has been studied extensively at the micro- and macro-scale, the nano-scopic level is perhaps the most illuminating as this is the length scale at which the individual constituents interact. ...

  9. High-speed photography of compressed human trabecular bone correlates whitening to microscopic damage

    E-Print Network [OSTI]

    Hansma, Paul

    ]. Trabecular bone is situated at the end of the long bones and in the spinal column, where it fills all of the inner vertebral space. In the long bones it transfers loads from joint surfaces onto the midshaft

  10. On the effect of x-ray irradiation on the deformation and fracture behavior of human cortical bone

    E-Print Network [OSTI]

    Barth, Holly D.

    2010-01-01

    reductions in plastic properties such as ultimate strength irradiation  on  the  plastic  properties  such  as  the 

  11. The significance of crack-resistance curves to the mixed-mode fracture toughness of human cortical bone

    E-Print Network [OSTI]

    Zimmermann, Elizabeth A.

    2010-01-01

    36], alumina [16], and zirconia [16]. The G c toughnessand wood, alumina, and zirconia, and the mode II toughness,36], alumina [16], and zirconia [16]. The G c toughness

  12. Original Full Length Article Mixed-mode toughness of human cortical bone containing a longitudinal crack in

    E-Print Network [OSTI]

    Ritchie, Robert

    of Materials Science and Engineering, University of California, Berkeley, CA 94720, USA a b s t r a c ta r t i strain-energy release rate, Gc, is plotted for several materials, specifi- cally alumina, zirconia (2012) 331­336 Corresponding author at: Department of Materials Science and Engineering, University

  13. Murine retroviral bone marrow transplantation models for the study of human myeloproliferative disorders.

    E-Print Network [OSTI]

    Gavrilescu, L Cristina; Van Etten, Richard A

    2008-01-01

    forceps Bone rongeurs Styrofoam dissecting board 21-Gthe animal’s feet to a Styrofoam dissecting board with 21-G

  14. The Macroscopic Cortical Unit

    E-Print Network [OSTI]

    Penny, Will

    The Macroscopic Brain Will Penny Cortical Unit Neural Mass Model Cell Populations Differential Will Penny 21st April 2011 #12;The Macroscopic Brain Will Penny Cortical Unit Neural Mass Model Cell as formulated in David et al. (2006). #12;The Macroscopic Brain Will Penny Cortical Unit Neural Mass Model Cell

  15. Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation

    E-Print Network [OSTI]

    Park, Sang-Hyug

    Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs ...

  16. Method for palliation of pain in human bone cancer using therapeutic tin-117m compositions

    DOE Patents [OSTI]

    Srivastava, Suresh C. (Setauket, NY); Meinken, George E. (Middle Island, NY); Mausner, Leonard F. (Stony Brook, NY); Atkins, Harold L. (Setauket, NY)

    1998-12-29

    The invention provides a method for the palliation of bone pain due to cancer by the administration of a unique dosage of a tin-117m (Sn-117m) stannic chelate complex in a pharmaceutically acceptable composition. In addition, the invention provides a method for simultaneous palliation of bone pain and radiotherapy in cancer patients using compositions containing Sn-117m chelates. The invention also provides a method for palliating bone pain in cancer patients using Sn-117m-containing compositions and monitoring patient status by imaging the distribution of the Sn-117m in the patients. Also provided are pharmaceutically acceptable compositions containing Sn-117m chelate complexes for the palliation of bone pain in cancer patients.

  17. Method for palliation of pain in human bone cancer using therapeutic tin-117m compositions

    DOE Patents [OSTI]

    Srivastava, S.C.; Meinken, G.E.; Mausner, L.F.; Atkins, H.L.

    1998-12-29

    The invention provides a method for the palliation of bone pain due to cancer by the administration of a unique dosage of a tin-117m (Sn-117m) stannic chelate complex in a pharmaceutically acceptable composition. In addition, the invention provides a method for simultaneous palliation of bone pain and radiotherapy in cancer patients using compositions containing Sn-117m chelates. The invention also provides a method for palliating bone pain in cancer patients using Sn-117m-containing compositions and monitoring patient status by imaging the distribution of the Sn-117m in the patients. Also provided are pharmaceutically acceptable compositions containing Sn-117m chelate complexes for the palliation of bone pain in cancer patients. 5 figs.

  18. Comparative study of the chondrogenic potential of human bone marrow stromal cells, neonatal chondrocytes and adult chondrocytes

    SciTech Connect (OSTI)

    Saha, Sushmita; Kirkham, Jennifer; NIHR Leeds Musculoskeletal Biomedical Research Unit, University of Leeds, Chapel Allerton Hospital, Leeds LS74SA ; Wood, David; Curran, Stephen; Yang, Xuebin; NIHR Leeds Musculoskeletal Biomedical Research Unit, University of Leeds, Chapel Allerton Hospital, Leeds LS74SA

    2010-10-22

    Research highlights: {yields} This study has characterised three different cell types under conditions similar to those used for autologous chondrocyte implantation (ACI) for applications in cartilage repair/regeneration. {yields} Compared for the first time the chondrogenic potential of neonatal chondrocytes with human bone marrow stromal cells (HBMSCs) and adult chondrocytes. {yields} Demonstrated that adult chondrocytes hold greatest potential for use in ACI based on their higher proliferation rates, lower alkaline phosphatise activity and enhanced expression of chondrogenic genes. {yields} Demonstrated the need for chondroinduction as a necessary pre-requisite to efficient chondrogenesis in vitro and, by extrapolation, for cell based therapy (e.g. ACI or cartilage tissue engineering). -- Abstract: Cartilage tissue engineering is still a major clinical challenge with optimisation of a suitable source of cells for cartilage repair/regeneration not yet fully addressed. The aims of this study were to compare and contrast the differences in chondrogenic behaviour between human bone marrow stromal cells (HBMSCs), human neonatal and adult chondrocytes to further our understanding of chondroinduction relative to cell maturity and to identify factors that promote chondrogenesis and maintain functional homoeostasis. Cells were cultured in monolayer in either chondrogenic or basal medium, recapitulating procedures used in existing clinical procedures for cell-based therapies. Cell doubling time, morphology and alkaline phosphatase specific activity (ALPSA) were determined at different time points. Expression of chondrogenic markers (SOX9, ACAN and COL2A1) was compared via real time polymerase chain reaction. Amongst the three cell types studied, HBMSCs had the highest ALPSA in basal culture and lowest ALPSA in chondrogenic media. Neonatal chondrocytes were the most proliferative and adult chondrocytes had the lowest ALPSA in basal media. Gene expression analysis revealed a difference in the temporal expression of chondrogenic markers which were up regulated in chondrogenic medium compared to levels in basal medium. Of the three cell types studied, adult chondrocytes offer a more promising cell source for cartilage tissue engineering. This comparative study revealed differences between the microenvironment of all three cell types and provides useful information to inform cell-based therapies for cartilage regeneration.

  19. Biodegradable synthetic bone composites

    DOE Patents [OSTI]

    Liu, Gao; Zhao, Dacheng; Saiz, Eduardo; Tomsia, Antoni P.

    2013-01-01

    The invention provides for a biodegradable synthetic bone composition comprising a biodegradable hydrogel polymer scaffold comprising a plurality of hydrolytically unstable linkages, and an inorganic component; such as a biodegradable poly(hydroxyethylmethacrylate)/hydroxyapatite (pHEMA/HA) hydrogel composite possessing mineral content approximately that of human bone.

  20. Effects of microstructure and water on the electrical potentials in bone induced by ultrasound irradiation

    SciTech Connect (OSTI)

    Tsuneda, H.; Matsukawa, S.; Takayanagi, S.; Matsukawa, M.; Mizuno, K.; Yanagitani, T.

    2015-02-16

    The healing mechanism of bone fractures by low intensity pulse ultrasound is yet to be fully understood. There have been many discussions regarding how the high frequency dynamic stress can stimulate numerous cell types through various pathways. As one possible initial process of this mechanism, we focus on the piezoelectricity of bone and demonstrate that bone can generate electrical potentials by ultrasound irradiation in the MHz range. We have fabricated ultrasonic bone transducers using bovine cortical bone as the piezoelectric device. The ultrasonically induced electrical potentials in the transducers change as a function of time during immersed ultrasonic pulse measurements and become stable when the bone is fully wet. In addition, the magnitude of the induced electrical potentials changes owing to the microstructure in the cortical bone. The potentials of transducers with haversian structure bone are higher than those of plexiform structure bone, which informs about the effects of bone microstructure on the piezoelectricity.

  1. The safety and efficacy of an injectable bone substitute in dental sockets demonstrated in a human clinical trial

    E-Print Network [OSTI]

    Boyer, Edmond

    in a water-soluble cellulose polymer carrier phase. It was used for filling bone defects after tooth extractions in eleven patients. The first objective of the study was to investigate the safety of the filler of the implanted areas were harvested and analyzed by using micro-computed tomography, non-decalcified histology

  2. Fibroblast growth factor 2 inhibits up-regulation of bone morphogenic proteins and their receptors during osteoblastic differentiation of human mesenchymal stem cells

    SciTech Connect (OSTI)

    Biver, Emmanuel, E-mail: ebiver@yahoo.fr [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France) [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France); Department of Rheumatology, Lille University Hospital, Roger Salengro Hospital, 59037 Lille cedex (France); Service of Bone Diseases, Department of Internal Medicine Specialties, University Hospital of Geneva, CH-1211 Geneva 14 (Switzerland); Soubrier, Anne-Sophie [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France) [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France); Department of Rheumatology, Lille University Hospital, Roger Salengro Hospital, 59037 Lille cedex (France); Thouverey, Cyril [Service of Bone Diseases, Department of Internal Medicine Specialties, University Hospital of Geneva, CH-1211 Geneva 14 (Switzerland)] [Service of Bone Diseases, Department of Internal Medicine Specialties, University Hospital of Geneva, CH-1211 Geneva 14 (Switzerland); Cortet, Bernard [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France) [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France); Department of Rheumatology, Lille University Hospital, Roger Salengro Hospital, 59037 Lille cedex (France); Broux, Odile [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France)] [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France); Caverzasio, Joseph [Service of Bone Diseases, Department of Internal Medicine Specialties, University Hospital of Geneva, CH-1211 Geneva 14 (Switzerland)] [Service of Bone Diseases, Department of Internal Medicine Specialties, University Hospital of Geneva, CH-1211 Geneva 14 (Switzerland); Hardouin, Pierre [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France)] [Physiopathology of Inflammatory Bone Diseases, EA 4490, University Lille North of France, Quai Masset, Bassin Napoleon, BP120, 62327 Boulogne sur Mer (France)

    2012-11-02

    Highlights: Black-Right-Pointing-Pointer FGF modulates BMPs pathway in HMSCs by down-regulating BMP/BMPR expression. Black-Right-Pointing-Pointer This effect is mediated by ERK and JNK MAPKs pathways. Black-Right-Pointing-Pointer Crosstalk between FGF and BMPs must be taken into account in skeletal bioengineering. Black-Right-Pointing-Pointer It must also be considered in the use of recombinant BMPs in orthopedic and spine surgeries. -- Abstract: Understanding the interactions between growth factors and bone morphogenic proteins (BMPs) signaling remains a crucial issue to optimize the use of human mesenchymal stem cells (HMSCs) and BMPs in therapeutic perspectives and bone tissue engineering. BMPs are potent inducers of osteoblastic differentiation. They exert their actions via BMP receptors (BMPR), including BMPR1A, BMPR1B and BMPR2. Fibroblast growth factor 2 (FGF2) is expressed by cells of the osteoblastic lineage, increases their proliferation and is secreted during the healing process of fractures or in surgery bone sites. We hypothesized that FGF2 might influence HMSC osteoblastic differentiation by modulating expressions of BMPs and their receptors. BMP2, BMP4, BMPR1A and mainly BMPR1B expressions were up-regulated during this differentiation. FGF2 inhibited HMSCs osteoblastic differentiation and the up-regulation of BMPs and BMPR. This effect was prevented by inhibiting the ERK or JNK mitogen-activated protein kinases which are known to be activated by FGF2. These data provide a mechanism explaining the inhibitory effect of FGF2 on osteoblastic differentiation of HMSCs. These crosstalks between growth and osteogenic factors should be considered in the use of recombinant BMPs in therapeutic purpose of fracture repair or skeletal bioengineering.

  3. Sleep Dynamics and Seizure Control in a Mesoscale Cortical Model

    E-Print Network [OSTI]

    Lopour, Beth Ann

    2009-01-01

    Contributions . . . . . . . . . 2 Mesoscale Cortical Modelstates in h e from the mesoscale cortical model, here- afterand Seizure Control in a Mesoscale Cortical Model by Beth

  4. Transgenic mice that express the human multidrug-resistance gene in bone marrow enable a rapid identification of agents that reverse drug resistance

    SciTech Connect (OSTI)

    Mickisch, G.H.; Merlino, G.T.; Galski, H.; Gottesman, M.M.; Pastan, I. )

    1991-01-15

    The development of preclinical models for the rapid testing of agents that circumvent multidrug resistance in cancer is a high priority of research on drug resistance. A common form of multidrug resistance in human cancer results from expression of the MDR1 gene, which encodes a M{sub r} 170,000 glycoprotein that functions as a plasma membrane energy-dependent multidrug efflux pump. The authors have engineered transgenic mice that express this multidrug transporter in their bone marrow and demonstrated that these animals are resistant to leukopenia by a panel of anticancer drugs including anthracyclines, vinca alkaloids, etoposide, taxol, and actinomycin D. Differential leukocyte counts indicate that both neutrophils and lympohcytes are pretected. Drugs such as cisplatin, methotrexate, and 5-fluorouracil, which are not handled by the multidrug transporter, produce bone marrow suppression in both normal and transgenic mice. The resistance conferred by the MDR1 gene can be circumvented in a dose-dependent manner by simultaneous administration of agents previously shown to be inhibitors of the multidrug transporter in vitro, including verapamil isomers, quinidine, and quinine. They conclude that MDR1-transgenic mice provide a rapid and reliable system to determine the bioactivity of agents that reverse multidrug resistance in animals.

  5. The effects of adult-onset alcoholism on cortical bone 

    E-Print Network [OSTI]

    Moe, Atha Louise

    2001-01-01

    -fed liquid control diet or rat pellet chow for either 8 or 14 weeks. An additional group of animals (alcohol cessation and pair-fed cessation) was fed the alcohol diet for 8 weeks with pair-fed partners receiving the liquid control diet. These animals were...

  6. Spectral Analysis and Connectivity of Porous Microstructures in Bone

    E-Print Network [OSTI]

    Golden, Kenneth M.

    that quantifies brine connectivity and its thermal evolution can also help assess the impact of osteoporosis on trabecular structure. Central to our approach is the spectral measure of a composite material, which contains, in dense cortical bone the pores can be sparse and disconnected, yet exhibit increasing volume fraction

  7. Auger electron spectroscopy for the determination of sex and age related Ca/P ratio at different bone sites

    SciTech Connect (OSTI)

    Balatsoukas, Ioannis; Kourkoumelis, Nikolaos; Tzaphlidou, Margaret [Department of Medical Physics, Medical School, University of Ioannina, Ioannina 45110 (Greece)

    2010-10-15

    The Ca/P ratio of normal cortical and trabecular rat bone was measured by Auger electron spectroscopy (AES). Semiquantitative analysis was carried out using ratio techniques to draw conclusions on how age, sex and bone site affect the relative composition of calcium and phosphorus. Results show that Ca/P ratio is not sex dependent; quite the opposite, bone sites exhibit variations in elemental stoichiometry where femoral sections demonstrate higher Ca/P ratio than rear and front tibias. Age-related changes are more distinct for cortical bone in comparison with the trabecular bone. The latter's Ca/P ratio remains unaffected from all the parameters under study. This study confirms that AES is able to successfully quantify bone mineral main elements when certain critical points, related to the experimental conditions, are addressed effectively.

  8. Potential role of 20S proteasome in maintaining stem cell integrity of human bone marrow stromal cells in prolonged culture expansion

    SciTech Connect (OSTI)

    Lu, Li; Song, Hui-Fang; Zhang, Wei-Guo; Liu, Xue-Qin; Zhu, Qian; Cheng, Xiao-Long; Yang, Gui-Jiao; Li, Ang; Xiao, Zhi-Cheng; Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Melbourne 3800

    2012-05-25

    Highlights: Black-Right-Pointing-Pointer Prolonged culture expansion retards proliferation and induces senescence of hBMSCs. Black-Right-Pointing-Pointer Reduced 20S proteasomal activity and expression potentially contribute to cell aging. Black-Right-Pointing-Pointer MG132-mediated 20S proteasomal inhibition induces senescence-like phenotype. Black-Right-Pointing-Pointer 18{alpha}-GA stimulates proteasomal activity and restores replicative senescence. Black-Right-Pointing-Pointer 18{alpha}-GA retains differentiation without affecting stem cell characterizations. -- Abstract: Human bone marrow stromal cells (hBMSCs) could be used in clinics as precursors of multiple cell lineages following proper induction. Such application is impeded by their characteristically short lifespan, together with the increasing loss of proliferation capability and progressive reduction of differentiation potential after the prolonged culture expansion. In the current study, we addressed the possible role of 20S proteasomes in this process. Consistent with prior reports, long-term in vitro expansion of hBMSCs decreased cell proliferation and increased replicative senescence, accompanied by reduced activity and expression of the catalytic subunits PSMB5 and PSMB1, and the 20S proteasome overall. Application of the proteasome inhibitor MG132 produced a senescence-like phenotype in early passages, whereas treating late-passage cells with 18{alpha}-glycyrrhetinic acid (18{alpha}-GA), an agonist of 20S proteasomes, delayed the senescence progress, enhancing the proliferation and recovering the capability of differentiation. The data demonstrate that activation of 20S proteasomes assists in counteracting replicative senescence of hBMSCs expanded in vitro.

  9. Cortical spatiotemporal plasticity in visual category learning

    E-Print Network [OSTI]

    Cortical spatiotemporal plasticity in visual category learning Yang Xu August 2013 CMU-ML-13-110 #12;#12;Cortical spatiotemporal plasticity in visual category learning Yang Xu August 2013 CMU-ML-13 of Philosophy c 2013 Yang Xu This research was sponsored by the National Institutes of Health under grant

  10. Elevated extracellular calcium increases expression of bone morphogenetic protein-2 gene via a calcium channel and ERK pathway in human dental pulp cells

    SciTech Connect (OSTI)

    Tada, Hiroyuki [Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575 (Japan)] [Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575 (Japan); Nemoto, Eiji, E-mail: e-nemoto@umin.ac.jp [Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575 (Japan)] [Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575 (Japan); Kanaya, Sousuke; Hamaji, Nozomu; Sato, Hisae; Shimauchi, Hidetoshi [Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575 (Japan)] [Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575 (Japan)

    2010-04-16

    Dental pulp cells, which have been shown to share phenotypical features with osteoblasts, are capable of differentiating into odontoblast-like cells and generating a dentin-like mineral structure. Elevated extracellular Ca{sup 2+}Ca{sub o}{sup 2+} has been implicated in osteogenesis by stimulating the proliferation and differentiation of osteoblasts; however, the role of Ca{sub o}{sup 2+} signaling in odontogenesis remains unclear. We found that elevated Ca{sub o}{sup 2+} increases bone morphogenetic protein (BMP)-2 gene expression in human dental pulp cells. The increase was modulated not only at a transcriptional level but also at a post-transcriptional level, because treatment with Ca{sup 2+} increased the stability of BMP-2 mRNA in the presence of actinomycin D, an inhibitor of transcription. A similar increase in BMP-2 mRNA level was observed in other human mesenchymal cells from oral tissue; periodontal ligament cells and gingival fibroblasts. However, the latter cells exhibited considerably lower expression of BMP-2 mRNA compared with dental pulp cells and periodontal ligament cells. The BMP-2 increase was markedly inhibited by pretreatment with an extracellular signal-regulated kinase (ERK) inhibitor, PD98059, and partially inhibited by the L-type Ca{sup 2+} channels inhibitor, nifedipine. However, pretreatment with nifedipine had no effect on ERK1/2 phosphorylation triggered by Ca{sup 2+}, suggesting that the Ca{sup 2+} influx from Ca{sup 2+} channels may operate independently of ERK signaling. Dental pulp cells do not express the transcript of Ca{sup 2+}-sensing receptors (CaSR) and only respond slightly to other cations such as Sr{sup 2+} and spermine, suggesting that dental pulp cells respond to Ca{sub o}{sup 2+} to increase BMP-2 mRNA expression in a manner different from CaSR and rather specific for Ca{sub o}{sup 2+} among cations.

  11. BONE CHARACTERIZATION ASME_Fatemi_chap13.indd 251 3/18/2008 5:01:36 AM

    E-Print Network [OSTI]

    quality. Assessment of mechanical properties of human bone in vivo by mechanical vibration analysis for the in vivo determination of bone mechanical characteristics. The potential of vibration analysis applied), to infer progressive me- chanical damage in human bone, in vitro. Standard measures of bone mechanical

  12. Computer modeling approach for microsphere-packed bone scaffold Pallavi Lal, Wei Sun*

    E-Print Network [OSTI]

    Sun, Wei

    bone graft [5,6], for structural and human cellular assessment of scaffolds for bone repair [7 modeling approach for constructing a three-dimensional microsphere-packed bone graft structure is presented packing model to determine the number of microspheres packed in a synthesized bone graft. The pore size

  13. Sex Differences in Long Bone Fatigue Using a Rat Model Luisa D. Moreno,1

    E-Print Network [OSTI]

    Waldman, Stephen D.

    response to fatigue, we also determined the creep that occurred during the fatigue test. From the creep progress (Fig. 1). Caler and Carter32 studied cortical bone creep behavior during fatigue testing. When adaptation. From these results, we hypothesized that creep was the underlying mechanism that accounted

  14. High-speed photography of the development of microdamage in trabecular bone during compression

    E-Print Network [OSTI]

    Hansma, Paul

    is situated at the end of the long bones and in the spinal column, where it fills all of the inner vertebral, osteoarthritic, and osteoporotic human vertebral trabecular bone compressed at high strain rates. Apparent

  15. Osteocytes and Bone Diseases 

    E-Print Network [OSTI]

    Ren, Yinshi

    2015-05-06

    For many centuries, the osteoblast is considered to be responsible for bone formation. It is also believed that an imbalance of osteoblasts (weak) and osteoclasts (strong) is the main cause for bone diseases such as ...

  16. Gender Effects on Cortical Thickness , Thompson PM1

    E-Print Network [OSTI]

    Thompson, Paul

    thickness was estimated voxel by voxel and projected as a local value (mm) onto the cortical surface, where brains that appears to involve the architecture of the cortical mantle. We detected significantly greater

  17. Cortical neuron cultures Plate and coverslip coating

    E-Print Network [OSTI]

    West, Anne

    Cortical neuron cultures 9/19/05 Plate and coverslip coating 1. PDL cat #P6407 from Sigma. Stock are always coated overnight at 37 in a 24 well plate. Plastic dishes can be coated for as little as 2 hours. PDL can be reused several times for up to 1-2 months, Store at 4 in between uses. Wash coated CS

  18. Sur Cross-modal plasticity and cortical development 1 Rewiring cortex: Cross-modal plasticity and its implications for cortical

    E-Print Network [OSTI]

    Sur, Mriganka

    Sur Cross-modal plasticity and cortical development 1 Rewiring cortex: Cross-modal plasticity-modal plasticity and cortical development No of text pages: 37 No of figures: 5 Please address correspondence to: 617-253-9829 Email: msur@ai.mit.edu #12;Sur Cross-modal plasticity and cortical development 2

  19. Spectral analysis and connectivity of porous microstructures in bone Kenneth M. Golden , N. Benjamin Murphy, Elena Cherkaev

    E-Print Network [OSTI]

    Cherkaev, Elena

    also help assess the impact of osteoporosis on trabecular structure. Central to our approach- structures, ranging from a solid network of connected trabeculae containing numerous connected pores, in dense cortical bone the pores can be sparse and disconnected, yet exhibit increasing volume fraction

  20. Bone fragments a body can make

    SciTech Connect (OSTI)

    Stout, S.D.; Ross, L.M. Jr. )

    1991-05-01

    Data obtained from various analytical techniques applied to a number of small bone fragments recovered from a crime scene were used to provide evidence for the occurrence of a fatality. Microscopic and histomorphometric analyses confirmed that the fragments were from a human skull. X-ray microanalysis of darkened areas on the bone fragments revealed a chemical signature that matched the chemical signature of a shotgun pellet recovered at the scene of the crime. The above findings supported the deoxyribonucleic acid (DNA) fingerprint evidence which, along with other evidence, was used to convict a man for the murder of his wife, even though her body was never recovered.

  1. Composites structures for bone tissue reconstruction

    SciTech Connect (OSTI)

    Neto, W.; Santos, João; Avérous, L.; Schlatter, G.; Bretas, Rosario

    2015-05-22

    The search for new biomaterials in the bone reconstitution field is growing continuously as humane life expectation and bone fractures increase. For this purpose, composite materials with biodegradable polymers and hydroxyapatite (HA) have been used. A composite material formed by a film, nanofibers and HA has been made. Both, the films and the non-woven mats of nanofibers were formed by nanocomposites made of butylene adipate-co-terephthalate (PBAT) and HA. The techniques used to produce the films and nanofibers were spin coating and electrospinning, respectively. The composite production and morphology were evaluated. The composite showed an adequate morphology and fibers size to be used as scaffold for cell growth.

  2. Electromagnetic signature of human cortical dynamics during wakefulness and sleep

    E-Print Network [OSTI]

    Destexhe, Alain

    . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 #12;4 2.2 From micro-scale to meso-scale to macro-scale . . . . . . . . . . . . . . . . . 30 2

  3. Modelling and simulation of acrylic bone cement injection and curing within the framework of vertebroplasty

    E-Print Network [OSTI]

    Landgraf, Ralf; Kolmeder, Sebastian; Lion, Alexander; Lebsack, Helena; Kober, Cornelia

    2013-01-01

    The minimal invasive procedure of vertebroplasty is a surgical technique to treat compression fractures of vertebral bodies. During the treatment liquid bone cement gets injected into the affected vertebral body and therein cures to a solid. In order to investigate the treatment and the impact of injected bone cement on the vertebra, an integrated modelling and simulation framework has been developed. The framework includes (i) the generation of computer models based on microCT images of human cancellous bone, (ii) CFD simulations of bone cement injection into the trabecular structure of a vertebral body as well as (iii) non-linear FEM simulations of the bone cement curing. Thereby, microstructural models of trabecular bone structures are employed. Furthermore, a detailed description of the material behaviour of acrylic bone cements is provided. More precisely, a non-linear fluid flow model is chosen for the representation of the bone cement behaviour during injection and a non-linear viscoelastic material mo...

  4. A Heat Kernel based Cortical Thickness Estimation Algorithm

    E-Print Network [OSTI]

    Wang, Yalin

    thickness, Heat Kernel, Tetrahedral Mesh, Streamline, False Discovery Rate 1 Introduction AlzheimerA Heat Kernel based Cortical Thickness Estimation Algorithm Gang Wang1,2 , Xiaofeng Zhang1.R.China Abstract. Cortical thickness estimation in magnetic resonance imag- ing (MRI) is an important technique

  5. The Development of Cortical Sensitivity to Visual Word Forms

    E-Print Network [OSTI]

    Wandell, Brian A.

    The Development of Cortical Sensitivity to Visual Word Forms Michal Ben-Shachar1,2 , Robert F. Dougherty1 , Gayle K. Deutsch1 , and Brian A. Wandell1 Abstract The ability to extract visual word forms longitudinal fMRI study to chart individual changes in cortical sensitivity to written words as reading de

  6. Influence of viscoelastic and viscous absorption on ultrasonic wave propagation in cortical bone: application to axial

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    and the surrounding soft tissues are attenuating media, which might affect the radiofrequency signals measured systems and media, 43.20.Mv Waveguides, wave propagation in tubes and ducts, 43.20.Px Transient radiation and scattering, 43.40.Rj Radiation from vibrating structures into fluid media, 43.35.Pt Surface waves in solids

  7. The harmful effects of late-onset alcohol consumption on cortical bone in aged rats 

    E-Print Network [OSTI]

    Bowlin, Julie Lee

    2001-01-01

    This study looked at the effects of late-onset alcohol consumption for 8 weeks on the aged rat model (15 months old). Thirty 15 month old female Fisher 344 rats were divided into three diet groups: Alcohol (n=9), pair-fed (n=9), and pellet (n=6...

  8. Invest in Your Bones Bone Mineral Calcium and Vitamin D

    E-Print Network [OSTI]

    Invest in Your Bones Bone Mineral Calcium and Vitamin D Leaflet 4 One common misconception is that calcium is only needed for growing children. In reality, both calcium and vitamin D are needed to maintain of calcium from your bones. Vitamin D promotes the absorption of calcium in the gut, which is necessary

  9. Computational Bodybuilding: Anatomically-based Modeling of Human Bodies Shunsuke Saito*

    E-Print Network [OSTI]

    Plotkin, Joshua B.

    , allowing us to produce human bodies with varied anthropometry, such as heights and bone lengths. Our goal

  10. Fracture, aging, and disease in bone J.W. Ager III

    E-Print Network [OSTI]

    Ritchie, Robert

    Fracture, aging, and disease in bone J.W. Ager III Materials Sciences Division, Lawrence Berkeley-known increase with age in fracture risk of human bone is essential. This also represents a challenge from accompanying the process of aging using appropriate multiscale experimental methods and relating them

  11. Immunodissection and culture of rabbit cortical collecting tubule cells

    SciTech Connect (OSTI)

    Spielman, W.S.; Sonnenburg, W.K.; Allen, M.L.; Arend, L.J.; Gerozissis, K.; Smith, W.L.

    1986-08-01

    A mouse monoclonal antibody designated IgG3 (rct-30) has been prepared that reacts specifically with an antigen on the surface of all cells comprising the cortical and medullary rabbit renal collecting tubule including the arcades. Plastic culture dishes coated with IgG3 (rct-30) were used to isolate collecting tubule cells from collagenase dispersions of rabbit renal cortical cells by immunoadsorption. Typically, 10W rabbit cortical collecting tubule (RCCT) cells were obtained from 5 g of renal cortex (2 kidneys). Between 20 and 30% of the RCCT cells were reactive with peanut lectin suggesting that RCCT cells are a mixture of principal and intercalated cells. Approximately 10X RCCT cells were obtained after 4 to 5 days in primary culture. Moreover, RCCT cells continued to proliferate after passaging with a doubling time of approx.32 h. RCCT cells passaged once and then cultured 4-5 days were found 1) to synthesize cAMP in response to arginine vasopressin (AVP), prostaglandin E2 (PGE2), isoproterenol, and parathyroid hormone, but not calcitonin, prostaglandin D2, or prostaglandin I, and 2) to release PGE2 in response to bradykinin but not arginine vasopressin or isoproterenol. The results indicate that cultured RCCT cells retain many of the hormonal, histochemical, and morphological properties expected for a mixture of principal and intercalated rabbit cortical collecting tubule epithelia. RCCT cells should prove useful both for studying hormonal interactions in the cortical collecting tubule and as a starting population for isolating intercalated collecting tubule epithelia.

  12. APPLICATIONS OF ALGEBRAIC MULTIGRID TO LARGE-SCALE FINITE ELEMENT ANALYSIS OF WHOLE BONE MICRO-MECHANICS ON THE IBM SP

    E-Print Network [OSTI]

    California at Berkeley, University of

    APPLICATIONS OF ALGEBRAIC MULTIGRID TO LARGE-SCALE FINITE ELEMENT ANALYSIS OF WHOLE BONE MICRO,5 Abstract. Accurate micro-finite element analyses of whole bones require the solution of large sets architectures. Key words. multigrid, trabecular bone, human vertebral body, finite element method, massively

  13. Development of Osteoinductive, High Porosity PolyHIPEs as Injectable Bone Grafts 

    E-Print Network [OSTI]

    Robinson, Jennifer Lindsey

    2014-08-25

    . Methods to modulate polyHIPE pore architecture, compressive properties, and degradation rates were established. Injectable polyHIPEs with pore sizes ranging from 1- 200 ?m, compressive properties comparable to human cancellous bone, and degradation...

  14. A laminar cortical model of stereopsis and three-dimensional surface perception

    E-Print Network [OSTI]

    Grossberg, Stephen

    A laminar cortical model of stereopsis and three-dimensional surface perception Stephen Grossberg University 677 Beacon Street, Boston, MA 02215, USA Running title: Laminar cortical model of depth perception;1 Abstract A laminar cortical model of stereopsis and later stages of 3D surface perception is developed

  15. Stereopsis and 3D Surface Perception by Spiking Neurons in Laminar Cortical Circuits

    E-Print Network [OSTI]

    Grossberg, Stephen

    Stereopsis and 3D Surface Perception by Spiking Neurons in Laminar Cortical Circuits: A Method, USA Running title: Spiking laminar cortical model of depth perception Neural Networks, in pressNAPSE program of DARPA (HR0011-09-C- 0001). #12;2 Abstract A laminar cortical model of stereopsis and 3D surface

  16. RESEARCH ARTICLE Shared and Idiosyncratic Cortical Activation Patterns in Autism

    E-Print Network [OSTI]

    Hasson, Uri

    RESEARCH ARTICLE Shared and Idiosyncratic Cortical Activation Patterns in Autism Revealed Under been documented in individuals with autism, the functional implications of these alterations remain activity in adults with autism during free-viewing of a popular audio­visual movie. Whereas these complex

  17. Functional and Anatomical Cortical Underconnectivity in Autism: Evidence

    E-Print Network [OSTI]

    Functional and Anatomical Cortical Underconnectivity in Autism: Evidence from an fMRI Study degrees. However, there were 3 indications of underconnectivity in the group with autism. First, within the autism group but not within the control group, the size of the genu of the corpus callosum

  18. Neurobiology of Disease Cortical Folding Abnormalities in Autism Revealed by

    E-Print Network [OSTI]

    Van Essen, David

    Neurobiology of Disease Cortical Folding Abnormalities in Autism Revealed by Surface-based morphometry across a range of autism spectrum disorders (7.5­18 years of age). We generated sulcal depth maps autism spectrum disorder subgroups: low-functioning autism, high-functioning autism, and Asperger

  19. Competing Sound Sources Reveal Spatial Effects in Cortical Processing

    E-Print Network [OSTI]

    Shinn-Cunningham, Barbara

    (an analog of mammalian primary auditory cortex) encode source identity. We find that the location, these results suggest that although spatial information is available, it is not the primary feature representedCompeting Sound Sources Reveal Spatial Effects in Cortical Processing Ross K. Maddox1,2 *, Cyrus P

  20. R326 Dispatch Nuclear migration: Cortical anchors for cytoplasmic dynein

    E-Print Network [OSTI]

    R326 Dispatch Nuclear migration: Cortical anchors for cytoplasmic dynein Kerry Bloom Nuclear body that rolls around at random inside the sack of a eukaryotic cell. Controlled nuclear movements they are required during budding. Nuclear migration in budding yeast was first proposed by Hartwell et al. [1], more

  1. 476 THE NEUROSCIENTIST Cortical Hierarchy Copyright 2004 Sage Publications

    E-Print Network [OSTI]

    Jouve, Bertrand

    476 THE NEUROSCIENTIST Cortical Hierarchy Copyright © 2004 Sage Publications ISSN 1073­8584 Understanding how the cerebral cortex processes infor­ mation is a major aim of neurobiology today with impor­directed projections that stem from infragranular layer neurons and terminate outside of layer 4. These findings were

  2. Relationship between alveolar bone measured by /sup 125/I absorptiometry with analysis of standardized radiographs: 2. Bjorn technique

    SciTech Connect (OSTI)

    Ortman, L.F.; McHenry, K.; Hausmann, E.

    1982-05-01

    The Bjorn technique is widely used in periodontal studies as a standardized measure of alveolar bone. Recent studies have demonstrated the feasibility of using /sup 125/I absorptiometry to measure bone mass. The purpose of this study was to compare /sup 125/I absorptiometry with the Bjorn technique in detecting small sequential losses of alveolary bone. Four periodontal-like defects of incrementally increasing size were produced in alveolar bone in the posterior segment of the maxilla of a human skull. An attempt was made to sequentially reduce the amount of bone in 10% increments until no bone remained, a through and through defect. The bone remaining at each step was measured using /sup 125/I absorptiometry. At each site the /sup 125/I absorptiometry measurements were made at the same location by fixing the photon source to a prefabricated precision-made occlusal splint. This site was just beneath the crest and midway between the borders of two adjacent teeth. Bone loss was also determined by the Bjorn technique. Standardized intraoral films were taken using a custom-fitted acrylic clutch, and bone measurements were made from the root apex to coronal height of the lamina dura. A comparison of the data indicates that: (1) in early bone loss, less than 30%, the Bjorn technique underestimates the amount of loss, and (2) in advanced bone loss, more than 60% the Bjorn technique overestimates it.

  3. Evidence for a Serum Factor That Initiates the Re-calcification of Demineralized Bone*S

    E-Print Network [OSTI]

    Price, Paul A.

    Evidence for a Serum Factor That Initiates the Re-calcification of Demineralized Bone*S Received to demineralization, and the re-calcified bone is palpably hard. Re-calcified bone mineral is com- parable- induced re-calcification of demineralized bone suggest that the serum calcification factor identified

  4. In vivo assessment of magnesium status in human body using accelerator-based neutron activation measurement of hands: A pilot study

    SciTech Connect (OSTI)

    Aslam; Pejovic-Milic, A.; McNeill, F. E.; Byun, S. H.; Prestwich, W. V.; Chettle, D. R. [Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, L8S 4K1 (Canada); Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, L8S 4K1, Canada and Department of Physics, Ryerson University, Toronto, Ontario, M5B 2K3 (Canada); Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, L8S 4K1 (Canada)

    2008-02-15

    Magnesium (Mg) is an element essential for many enzymatic reactions in the human body. Various human and animal studies suggest that changes in Mg status are linked to diseases such as cardiac arrhythmia, coronary heart disease, hypertension, premenstrual syndrome, and diabetes mellitus. Thus, knowledge of Mg levels in the human body is needed. A direct measurement of human blood serum, which contains only 0.3% of the total body Mg, is generally used to infer information about the status of Mg in the body. However, in many clinical situations, Mg stored in large levels, for example in bones, muscles, and soft tissues, needs to be monitored either to evaluate the efficacy of a treatment or to study the progression of diseases associated with the deficiency of total body Mg. This work presents a feasibility study of a noninvasive, in vivo neutron activation analysis (IVNAA) technique using the {sup 26}Mg(n,{gamma}){sup 27}Mg reaction to measure Mg levels in human hands. The technique employs the McMaster University high beam current Tandetron accelerator hand irradiation facility and an array of eight NaI (T1) detectors arranged in a 4{pi} geometry for delayed counting of the 0.844 and 1.014 MeV gamma rays emitted when {sup 27}Mg decays in the irradiated hand. Mg determination in humans using IVNAA of hands has been demonstrated to be feasible, with effective doses as low as one-quarter of those delivered in chest x rays. The overall experimental uncertainty in the measurements is estimated to be approximately 5% (1{sigma}). The results are found to be in the range of the in vitro measurements reported for other cortical bones collected from different sites of the human skeleton, which confirms that this technique mainly provides a measure of the amount of Mg in hand bones. The average concentration of Mg determined in human hands is 10.96{+-}1.25 ({+-}1 SD) mg Mg/g Ca. The coefficient of variation (11%) observed in this study is comparable with or lower than several studies using in vitro measurements reported in the literature and therefore allows for a quantitative intersubject comparison, even if to a limited extent. The features of the developed technique such as its simplicity, rapidity, accuracy, robustness, noninvasive nature, and very effective use of radiation doses, present the technique as a viable diagnostic tool available for trial in a clinical environment.

  5. From genes to folds: a review of cortical gyrification theory

    E-Print Network [OSTI]

    Ronan, Lisa; Fletcher, Paul C.

    2014-12-16

    observe that it could be extended a considerable distance that it had a smooth covering of feathers with conspicuous markings, that it was operated by powerful muscles, and that strength and lightness were prominent fea- tures of its construction... of cortical development (Fig. 2). This is fundamentally based on the radial unit hypothesis which postulates that development begins with a period of symmetric division of cells along the ventricular wall. This dramatically increases the number of founder...

  6. Synthetic reverberating activity patterns embedded in networks of cortical neurons

    E-Print Network [OSTI]

    Roni Vardi; Avner Wallach; Evi Kopelowitz; Moshe Abeles; Shimon Marom; Ido Kanter

    2012-03-26

    Synthetic reverberating activity patterns are experimentally generated by stimulation of a subset of neurons embedded in a spontaneously active network of cortical cells in-vitro. The neurons are artificially connected by means of conditional stimulation matrix, forming a synthetic local circuit with a predefined programmable connectivity and time-delays. Possible uses of this experimental design are demonstrated, analyzing the sensitivity of these deterministic activity patterns to transmission delays and to the nature of ongoing network dynamics.

  7. Bone Mineral Density, Bone Turnover, and Systemic Inflammation in Non-cirrhotics with Chronic Hepatitis C

    E-Print Network [OSTI]

    Lai, JC; Shoback, DM; Zipperstein, J; Lizaola, B; Tseng, S; Terrault, NA

    2015-01-01

    Mun˜oz-Torres M, et al. Bone mineral density, serum insulin-et al. Osteoporosis and bone mineral metabolism disorders in1069-9. 11. George J. Bone mineral density and disorders of

  8. Biomimetic hydroxyapatite as a new consolidating agent for archaeological bone

    E-Print Network [OSTI]

    North, Alexis

    2014-01-01

    R.E.M.  2002.  “Bone  Diagenesis:  An  Overview  of  2000.  “Patterns  of  Diagenesis  in  Bone  I:  The  element  Studies  of  Diagenesis  in  Prehistoric  Bone. ”  

  9. Biomechanics and cortical representation of whisking in the rat vibrissa system

    E-Print Network [OSTI]

    Hill, Daniel Nicholas

    2009-01-01

    Zeigler HP, Kleinfeld D. Biomechanics of the vibrissa motorJH, Hartmann MJ (2006) Biomechanics: Robotic whiskers usedOF CALIFORNIA, SAN DIEGO Biomechanics and Cortical

  10. Reduced cortical activity impairs development and plasticity after neonatal hypoxia ischemia

    E-Print Network [OSTI]

    2015-01-01

    lower in the ipsilateral HI hemisphere compared with thethalamo- notoxin, neonatal HI may involve lower corticalHanganu et After neonatal HI, we also see reduced glutamate

  11. Asymmetric projections of the arcuate fasciculus to the temporal cortex underlie lateralized language function in the human brain

    E-Print Network [OSTI]

    Takaya, Shigetoshi

    The arcuate fasciculus (AF) in the human brain has asymmetric structural properties. However, the topographic organization of the asymmetric AF projections to the cortex and its relevance to cortical function remain unclear. ...

  12. Positive modulator of bone morphogenic protein-2

    DOE Patents [OSTI]

    Zamora, Paul O. (Gaithersburg, MD); Pena, Louis A. (Poquott, NY); Lin, Xinhua (Plainview, NY); Takahashi, Kazuyuki (Germantown, MD)

    2009-01-27

    Compounds of the present invention of formula I and formula II are disclosed in the specification and wherein the compounds are modulators of Bone Morphogenic Protein activity. Compounds are synthetic peptides having a non-growth factor heparin binding region, a linker, and sequences that bind specifically to a receptor for Bone Morphogenic Protein. Uses of compounds of the present invention in the treatment of bone lesions, degenerative joint disease and to enhance bone formation are disclosed.

  13. The bending and dynamic mechanical properties of cortical bone: the effects of sodium fluoride and the relationship to physical properties 

    E-Print Network [OSTI]

    McCurdy-Rahn, Megan Calista

    1998-01-01

    treatment reduced the dynamic storage and three-point bending moduli, the yield and ultimate strengths, and ratio of yield to ultimate strength. This protocol also increased the tan 8, loss modulus, energy absorbed to failure, and percent of energy absorbed...

  14. Cortical Map Plasticity Improves Learning but Is Not Necessary for Improved Performance

    E-Print Network [OSTI]

    Kilgard, Michael P.

    Neuron Article Cortical Map Plasticity Improves Learning but Is Not Necessary for Improved.02.038 SUMMARY Cortical map plasticity is believed to be a key substrate of perceptual and skill learning of the cholinergic nucleus basalis to induce auditory cortex map plasticity outside of a behavioral context. Our

  15. On the Relationship Between Joint Angular Velocity and Motor Cortical Discharge During Reaching

    E-Print Network [OSTI]

    Moran, Daniel

    On the Relationship Between Joint Angular Velocity and Motor Cortical Discharge During Reaching G, and Andrew B. Schwartz. On the relationship between joint angular velocity and motor cortical discharge angular velocities. Hand velocity is considered a parameter of extrinsic space because it is measured

  16. Reply: Lithium and Increased Cortical Gray Matter--More Tissue or More Water?

    E-Print Network [OSTI]

    Thompson, Paul

    Reply: Lithium and Increased Cortical Gray Matter--More Tissue or More Water? To the Editor: W e cortices, in lithium-treated patients with bipolar disorder, relative to healthy control subjects (1). Dr patients. Although lithium's effects on body water homeostasis (2) are important to consider, the absence

  17. A 3D Volumetric Laplace-Beltrami Operator based Cortical Thickness Estimation Method

    E-Print Network [OSTI]

    Wang, Yalin

    A 3D Volumetric Laplace-Beltrami Operator based Cortical Thickness Estimation Method Gang Wanga the cortical tetrahedral mesh, we adopt the heat kernel [1] based on volumetric Laplace-Beltrami operator can construct the discrete volumetric Laplace-Beltrami operator under the Dirichlet boundary condition

  18. Distinct roles for inhibitory neuron subtypes in cortical circuits : an examination of their structure, function, and connectivity

    E-Print Network [OSTI]

    Runyan, Caroline A. (Caroline Anne)

    2012-01-01

    Parvalbumin-containing (PV+) neurons and somatostatin-containing (SOM+) neurons are two key cortical inhibitory cell classes that are poised to play distinct computational roles in cortical circuits: PV+ neurons form ...

  19. Bone mineral density and fractures in older men with chronic obstructive pulmonary disease or asthma

    E-Print Network [OSTI]

    Dam, T.-T.; Harrison, S.; Fink, H. A.; Ramsdell, J.; Barrett-Connor, E.

    2010-01-01

    x ORIGINAL ARTICLE Bone mineral density and fractures inwas associated with lower bone mineral density (BMD) at theKeywords Bone loss . Bone mineral density . Elderly .

  20. Digital electronic bone growth stimulator

    DOE Patents [OSTI]

    Kronberg, J.W.

    1993-01-01

    The present invention relates to the electrical treatment of biological tissue. In particular, the present invention discloses a device that produces discrete electrical pulse trains for treating osteoporosis and accelerating bone growth. According to its major aspects and broadly stated, the present invention consists of an electrical circuit configuration capable of generating Bassett-type waveforms shown with alternative signals provide for the treatment of either fractured bones or osteoporosis. The signal generator comprises a quartz clock, an oscillator circuit, a binary divider chain, and a plurality of simple, digital logic gates. Signals are delivered efficiently, with little or no distortion, and uniformly distributed throughout the area of injury. Perferably, power is furnished by widely available and inexpensive radio batteries, needing replacement only once in several days. The present invention can be affixed to a medical cast without a great increase in either weight or bulk. Also, the disclosed stimulator can be used to treat osteoporosis or to strengthen a healing bone after the cast has been removed by attaching the device to the patient`s skin or clothing.

  1. Bioactive Glass Scaffolds for Bone Regeneration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bioactive Glass Scaffolds for Bone Regeneration Print Natural materials are renowned for their unique combination of outstanding mechanical properties and exquisite microstructure....

  2. Composite bone substitutes prepared by two methods

    E-Print Network [OSTI]

    Lee, Hoe Yun; Lee, Hoe Yun

    2012-01-01

    Composites via Freeze Casting for Bone Tissue Engineering,”A.P. Tomsia, “Freeze casting of hydroxyapatite scaffolds forcamphene- based freeze casting,” Materials Letters 61(11-

  3. PPARs in Bone: The Role in Bone Cell Differentiation and Regulation of Energy Metabolism

    E-Print Network [OSTI]

    Toledo, University of

    PPARs in Bone: The Role in Bone Cell Differentiation and Regulation of Energy Metabolism Beata of bone homeostasis and energy metabolism. Peroxisome proliferator-activated receptors (PPARs) represent a family of proteins that control energy turnover in adipose, liver, and muscle tissue. These proteins also

  4. Hydroxyapatite-binding peptides for bone growth and inhibition

    DOE Patents [OSTI]

    Bertozzi, Carolyn R. (Berkeley, CA); Song, Jie (Shrewsbury, MA); Lee, Seung-Wuk (Walnut Creek, CA)

    2011-09-20

    Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp).sub.x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals .about.40 nm in size.

  5. Mapping bone changes at the proximal femoral cortex of postmenopausal women in response to alendronate and teriparatide alone, combined or sequentially

    E-Print Network [OSTI]

    Whitmarsh, Tristan; Treece, Graham M.; Gee, Andrew H.; Poole, Kenneth E. S.

    2015-06-15

    , it is important to bear in mind that TPTD has not been shown to prevent hip fracture in adequately powered clinical trials. Based on cost and clinical effectiveness, the National Institute for Health and Care Excellence (NICE) in the UK recommends ALN as a... depicts this at a simulated histological resolution (top panel, where new bone formed by teriparatide is shown in green). The lower two panels indicate what this process looks like to our cortical parameter measurements, with the first row of images...

  6. Self-Control of Avoidance Motivation: Implications for Understanding Frontal Cortical Asymmetry 

    E-Print Network [OSTI]

    Kelley, Nicholas J

    2015-08-10

    Self-control involves the inhibition of dominant response tendencies. Most research on self-control has examined the inhibition of approach-motivated tendencies, and previous research has found that right frontal cortical asymmetry facilitates...

  7. TOWARDS A UNIFIED THEORY OF NEOCORTEX: Laminar Cortical Circuits for Vision and Cognition

    E-Print Network [OSTI]

    Grossberg, Stephen

    TOWARDS A UNIFIED THEORY OF NEOCORTEX: Laminar Cortical Circuits for Vision and Cognition Stephen laminar neocortical circuits give rise to biological intelligence. These circuits embody two new and revolutionary computational paradigms: Complementary Computing and Laminar Computing. Circuit properties include

  8. Laminar and Columnar Distribution of Geniculo-cortical Fibers in the Macaque Monkey

    E-Print Network [OSTI]

    Hubel, David

    Laminar and Columnar Distribution of Geniculo-cortical Fibers in the Macaque Monkey DAVID H-Heimer modiikation of the Nauta method. We hoped to correlate the laminar distribution of axon terminals

  9. Maximum a Posteriori Models for Cortical Modeling: Feature Detectors, Topography and Modularity

    E-Print Network [OSTI]

    Weber, Cornelius

    Maximum a Posteriori Models for Cortical Modeling: Feature Detectors, Topography and Modularity Modeling: Feature Detectors, Topography and Modularity PhD Thesis by Cornelius Weber, Berlin, July 31, 2000

  10. Examining Associations between Emotional Facial Expressions, Relative Left Frontal Cortical Activity, and Task Persistence 

    E-Print Network [OSTI]

    Price, Thomas

    2012-10-19

    Past research associated relative left frontal cortical activity with approach motivation, or the inclination to move toward a stimulus, as well as positive affect. Work with anger, a negative emotion often high in approach, helped clarify the role...

  11. Implied motion activation in cortical area MT can be explained by visual low-level features

    E-Print Network [OSTI]

    Oram, Mike

    ForReview Only Implied motion activation in cortical area MT can be explained by visual low Neuroscience #12;ForReview Only 1 Implied motion activation in cortical area MT can be explained by visual low, The Netherlands Page 1 of 51 Jounal of Cognitive Neuroscience 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

  12. Approach- and Withdrawal-Oriented Responses to Social Rejection: The Role of Asymmetrical Frontal Cortical Activity 

    E-Print Network [OSTI]

    Peterson, Carly Kathryn

    2011-02-22

    - AND WITHDRAWAL-ORIENTED RESPONSES TO SOCIAL REJECTION: THE ROLE OF ASYMMETRICAL FRONTAL CORTICAL ACTIVITY A Thesis by CARLY KATHRYN PETERSON Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE December 2009 Major Subject: Psychology APPROACH- AND WITHDRAWAL-ORIENTED RESPONSES TO SOCIAL REJECTION: THE ROLE OF ASYMMETRICAL FRONTAL CORTICAL ACTIVITY A Thesis by CARLY KATHRYN...

  13. Histologic Comparison of Regenerate Bone Produced from Dentate Versus Edentulous Transport Discs in Bone Transport Distraction Osteogenesis 

    E-Print Network [OSTI]

    Sevilla Gaitan, Carlos

    2013-12-10

    Purpose: The purpose of this research was to quantify the number of blood vessels and nerves and mineral apposition rate (MAR) in native bone and compare it to the regenerate bone produced by bone transport distraction osteogenesis (BTDO...

  14. Investigation into mechanical properties of bone and its main constituents

    E-Print Network [OSTI]

    Evdokimenko, Ekaterina

    2012-01-01

    Kinetic studies of bone demineralization at different HClKinetic studies of the demineralization and deproteinationJ. McKittrick, “Bone demineralization and deproteination

  15. Elastic anisotropy of bone Biomechanics, BME 315

    E-Print Network [OSTI]

    Lakes, Roderic

    17 22 Glong 3.6 5.3 0.58 0.30 Etransv 11.5 15 Gtr 3.3 6.3 0.31 0.11 Etransv 11.5 12 Gtr 3.3 7.0 0 moduli of bone, in Mechanical Properties of Bone, Joint ASME-ASCE Applied Mechanics, Fluids Engineering

  16. Tracking cortical entrainment in neural activity: Auditory processes in human temporal cortex

    E-Print Network [OSTI]

    Thwaites, Andrew; Nimmo-Smith, Ian; Fonteneau, Elisabeth; Patterson, Roy D.; Buttery, Paula; Marslen-Wilson, William D.

    2015-02-10

    the vowel that we perceive. A good estimate of the glottal period in speech sounds is provided by the dominant lag in the autocor- relation of the wave (Licklider, 1956; Meddis and Hewitt, 1991). In the auditory system, in the second stage of processing, a... deviations (2?) from the mean of the null-distribution (assuming the distribution’s normality). In this paper, we decrease ? to the equivalent of 5? (p ? 2.9 × 10?7), to ensure that the evidence we present for the expression of partic- ular models...

  17. Deep analysis of perception through dynamic structures that emerge in cortical activity from self-regulated noise

    E-Print Network [OSTI]

    Freeman, Walter J III

    2009-01-01

    dynamic structures  that emerge in cortical activity from modulation (AM) patterns emerge in the ECoG, which are

  18. Investigation into mechanical properties of bone and its main constituents

    E-Print Network [OSTI]

    Evdokimenko, Ekaterina

    2012-01-01

    treatment of intrabony periodontal defects: 6 year resultsbones, to repair periodontal defects, bone removal from

  19. Mandible versus Long Bone Marrow Cells

    E-Print Network [OSTI]

    Chaichanasakul, Thawinee

    2012-01-01

    3-3 microCT analysis of gelatin sponge seeded with long boneFigure 3-4 H&E staining of gelatin sponge seeded with longprocess (Djagny et al. 2001). Gelatin- based sponge prepared

  20. Composite gelatin delivery system for bone regeneration

    E-Print Network [OSTI]

    Hager, Elizabeth A. (Elizabeth Ann)

    2005-01-01

    In this thesis, the chemical/mechanical properties and biocompatibility of gelatin were investigated to produce a gelatin scaffold for the release of bone morphogenetic proteins (BMPs) from composite particles. This delivery ...

  1. Nanoscale Surface Topography to Guide Bone Growth

    E-Print Network [OSTI]

    Nanoscale Surface Topography to Guide Bone Growth P R O J E C T L E A D E R : Jirun Sun (American T S Designed and fabricated devices with nanoscale surface topography. Controlled cell alignment by varying

  2. Microdamage accumulation in bovine trabecular bone

    E-Print Network [OSTI]

    Moore, Tara L. Arthur (Tara Lee Arthur), 1972-

    2001-01-01

    When bone is loaded beyond its failure point, it develops damage in the form of microcracks. Normally, microcracks are repaired by the remodeling process, limiting the number of in vivo microcracks. However, if the rate ...

  3. Mechanical bone strength in the proximal tibia 

    E-Print Network [OSTI]

    Prommin, Danu

    2000-01-01

    Six bone slices from one canine tibia were tested by compressive load to determine the ultimate strength as a function of distance from the knee. Both the ultimate compressive strength and overall modulus of elasticity of ...

  4. Cortical microtubule nucleation can organise the cytoskeleton of $Drosophila$ oocytes to define the anteroposterior axis

    E-Print Network [OSTI]

    Philipp Khuc Trong; Hélène Doerflinger; Jörn Dunkel; Daniel St. Johnston; Raymond E. Goldstein

    2015-09-26

    Many cells contain non-centrosomal arrays of microtubules (MT), but the assembly, organisation and function of these arrays are poorly understood. We present the first theoretical model for the non-centrosomal MT cytoskeleton in $Drosophila$ oocytes, in which $bicoid$ and $oskar$ mRNAs become localised to establish the anterior-posterior body axis. Constrained by experimental measurements, the model shows that a simple gradient of cortical MT nucleation is sufficient to reproduce the observed MT distribution, cytoplasmic flow patterns and localisation of $oskar$ and naive $bicoid$ mRNAs. Our simulations exclude a major role for cytoplasmic flows in localisation and reveal an organisation of the MT cytoskeleton that is more ordered than previously thought. Furthermore, modulating cortical MT nucleation induces a bifurcation in cytoskeletal organisation that accounts for the phenotypes of polarity mutants. Thus, our three-dimensional model explains many features of the MT network and highlights the importance of differential cortical MT nucleation for axis formation.

  5. Troyer et al. 54 Figure 2. Gaborshaped cortical RFs. Lighter grays to white: positive values of Gabor

    E-Print Network [OSTI]

    Columbia University

    ­center cells. A. A full Gabor function, used to determine LGN inputs to a cortical cell in the conceptual model from the full Gabor (see Methods). These receptive fields are 'typical'; different cortical cells may of Gabor function, corresponding to weights of ON­center LGN cells with centers at corresponding spatial

  6. WHY HAVE MULTIPLE CORTICAL AREAS? H. B. BARLOW

    E-Print Network [OSTI]

    Born, Richard

    -arranged, and some possible rules of projection for forming such images are explored. It is also suggested that all under-estimated the potentialities of nerves if, as Abeles (1082) and von der Malsberg (1981) suggest #12;82 H. B. Table I. Comparison between computer and brain Motorola VME/IO Human brain Architecture

  7. Cortical Activation During Spatiotemporal Processing in the Infant Brain 

    E-Print Network [OSTI]

    Armstrong, Jennifer R.

    2010-01-14

    visual cortex (O1), posterior parietal cortex (P3), lateral occipital (T5), and inferior temporal (T3) in awake human infants aged 5.5 months while they view either a spatiotemporaldiscontinuity event or a control event. Three major predictions were made...

  8. ORIGINAL PAPER Cortical Variability in the Sensory-Evoked Response in Autism

    E-Print Network [OSTI]

    Behrmann, Marlene

    ORIGINAL PAPER Cortical Variability in the Sensory-Evoked Response in Autism Sarah M. Haigh · David York 2014 Abstract Previous findings have shown that individuals with autism spectrum disorder (ASD-functioning adults with autism. Participants were presented with visual, somatosensory and auditory stimuli

  9. Reply: Lithium and Increased Cortical Gray Matter--More Tissue or More Water?

    E-Print Network [OSTI]

    Thompson, Paul

    Reply: Lithium and Increased Cortical Gray Matter--More Tissue or More Water? To the Editor: W e. Regenold voices some disappointment that we did not determine whether an increase in brain tissue water patients. Although lithium's effects on body water homeostasis (2) are important to consider, the absence

  10. Laminar Cortical Dynamics of Cognitive and Motor Working Memory, Sequence Learning and Performance

    E-Print Network [OSTI]

    Grossberg, Stephen

    Laminar Cortical Dynamics of Cognitive and Motor Working Memory, Sequence Learning and Performance cells, cerebral cortex, laminar computing * Authors are listed in alphabetical order. 1 Supported share the same type of circuit design. It proposes how the laminar circuits of lateral prefrontal cortex

  11. Targeted insult to subsurface cortical blood vessels using ultrashort laser pulses: three models of stroke

    E-Print Network [OSTI]

    Cai, Long

    Targeted insult to subsurface cortical blood vessels using ultrashort laser pulses: three models a vessel for injury and to measure blood-flow dynamics. We irradiated the vessel with high-fluence, ultrashort laser pulses and achieved three forms of vascular insult. (i) Vessel rupture was induced

  12. Intraoperative Brain Shift and Deformation: A Quantitative Analysis of Cortical Displacement in 28 Cases

    E-Print Network [OSTI]

    Frey, Pascal

    Intraoperative Brain Shift and Deformation: A Quantitative Analysis of Cortical Displacement in 28 of surgery, the nature of the cranial opening, the region of the brain involved, the duration of surgery resulting from misregistration during the course of surgery is warranted. Key words: brain shift, computer

  13. Cortical plasticity induced by transplantation of embryonic somatostatin or parvalbumin interneurons

    E-Print Network [OSTI]

    Stryker, Michael

    Cortical plasticity induced by transplantation of embryonic somatostatin or parvalbumin plasticity. We recently have shown that transplantation of GABAergic precursors from the embryonic medial, robust plasticity was observed in transplants contain- ing PV+ cells but in which the majority of SST

  14. Suppressing unwanted memories reduces their unconscious influence via targeted cortical inhibition

    E-Print Network [OSTI]

    Henson, Rik

    . These findings suggest a neurobio- logical model of how motivated forgetting affects the unconscious expressionSuppressing unwanted memories reduces their unconscious influence via targeted cortical inhibition memories can continue to exert strong unconscious effects that may compromise mental health. Here we show

  15. Liquid Computing in a Simplified Model of Cortical Layer IV: Learning to Balance a Ball

    E-Print Network [OSTI]

    Maass, Wolfgang

    Probst1,3 , Wolfgang Maass2 , Henry Markram1 , and Marc-Oliver Gewaltig1 1 Blue Brain Project, ´Ecole that the cortical network can robustly learn this task using a supervised learning rule that penalizes the error function is then constructed by readout neurons or action cells which compute a weighted sum of the high

  16. Short Communication Categorical effects in fricative perception are reflected in cortical source

    E-Print Network [OSTI]

    Daume III, Hal

    modeling a b s t r a c t Previous research in speech perception has shown that category information affects that these phonological cate- gories strongly influence how speech is perceived: category information allows for betterShort Communication Categorical effects in fricative perception are reflected in cortical source

  17. Neural interactions between motor cortical hemispheres during bimanual and unimanual arm movements

    E-Print Network [OSTI]

    Donchin, Opher

    Neural interactions between motor cortical hemispheres during bimanual and unimanual arm movements to address this issue, we simultaneously recorded neuronal activity at multiple sites within the arm area related to the mode of coupling between the two arms. Both the correlations between the movements

  18. Pictures of Appetizing Foods Activate Gustatory Cortices for Taste and Reward

    E-Print Network [OSTI]

    Barsalou, Lawrence W.

    Pictures of Appetizing Foods Activate Gustatory Cortices for Taste and Reward W. Kyle Simmons1 viewed pictures of appetizing foods (along with pictures of locations for comparison) during event-related fMRI. Compared to location pictures, food pictures activated the right insula/operculum and the left

  19. Regulated Nuclear Trafficking of the Homeodomain Protein Otx1 in Cortical Neurons

    E-Print Network [OSTI]

    McConnell, Susan

    Regulated Nuclear Trafficking of the Homeodomain Protein Otx1 in Cortical Neurons Y. Alex Zhang,1 in the rat ventricular zone, and remains cytoplasmic as neurons migrate and begin to differentiate. Nuclear, that the N-terminus of Otx1 is nec- essary for nuclear import, and that a putative nuclear localization

  20. Detecting Cortical Surface Regions in Structural MR Data Biswajit Bose1,

    E-Print Network [OSTI]

    Fisher III, John

    Detecting Cortical Surface Regions in Structural MR Data Biswajit Bose1, , John Fisher1 , Bruce on a challenging ex-vivo structural MR dataset for detection of Brodmann area 17. 1. Introduction Detection-resolution MR volume. Figure 1 shows a slice through structural MR data of the primary vi- sual cortex, where BA

  1. The effect of three hemostatic agents on early bone healing in an animal model

    E-Print Network [OSTI]

    2010-01-01

    B, Sjogren S: Effects of bone wax on rabbit cranial boneRR: The effect of bone wax on the healing of experimentaland healing using bone wax and a soluble polymer material.

  2. Early extrastriate activity without primary visual cortex in humans B. Rossiona, b,*, B. de Gelderb, c

    E-Print Network [OSTI]

    Rossion, Bruno

    to the primary visual cortex (V1) destroys the major source of anatomical input to extrastriate cortical areas (VEarly extrastriate activity without primary visual cortex in humans B. Rossiona, b,*, B. de Gelderb information reaches extrastriate areas, by means of event- related potentials (ERPs) we tracked the temporal

  3. Evidence for Interhemispheric Processing of Inputs From the Hands in Human S2 and PV

    E-Print Network [OSTI]

    Krubitzer, Leah A.

    Evidence for Interhemispheric Processing of Inputs From the Hands in Human S2 and PV ELIZABETH S2 and PV. J Neurophysiol 85: 2236­2244, 2001. In the present investigation, we identified cortical somatosensory (S2) and the parietal ventral (PV) areas, was significantly larger for bilateral stimulation than

  4. Cellular and molecular immunotherapeutics derived from the bone marrow stroma

    E-Print Network [OSTI]

    Parekkadan, Biju

    2008-01-01

    The bone marrow contains a multipotent stromal cell, commonly referred to as a mesenchymal stem cell (MSC). There has been recent interest in the clinical use of MSCs for cell-based therapy because: (1) bone marrow aspiration ...

  5. BONE LOSS IN RELATION TO HYPOTHALAMIC ATROPHY IN ALZHEIMER'S DISEASE

    E-Print Network [OSTI]

    Loskutova, Natalia Y.

    2011-05-12

    . The body of presented work extends the literature on a relationship between bone loss and AD. Overall, the presented work provides initial evidence that accelerated bone loss observed in individuals in the early stages of AD may be partially due...

  6. Mechanical loading attenuates loss of bone mass and bone strength induced by immobilization and calcium-deficiency 

    E-Print Network [OSTI]

    Inman, Cynthia Lynn

    1996-01-01

    Immobilization and calcium-deficiency have been documented to cause a decrease in strength and bone mineral loss, and exercise is known to strengthen bone. The purpose of this study was to determine the effects of mechanical ...

  7. Preparation of Bone Sam-ples for DNA Extraction: A

    E-Print Network [OSTI]

    , such as hammering frozen bone sealed in plastic freezer bags, have proven unreliable; shards of bone tend to pierce abrasive agent was included with the bone sample (in our case, sterilized glass fragments) to en- sure even-and-bolt as- sembly for reuse with other samples. However, considering the low cost of the nuts and bolts

  8. DYNAMICS OF FUNCTIONAL CONNECTIVITY WITHIN CORTICAL MOTOR NETWORK DURING MOTOR LEARNING IN STROKE - CORRELATIONS WITH "TRUE" MOTOR RECOVERY

    E-Print Network [OSTI]

    BANI-AHMED, ALI

    2013-08-31

    investigate the changes in cortical motor function at two levels, regional (micro-circuitry, regional activation) and network (macro-circuitry, functional connectivity), following an arm-focused motor training in chronic stroke survivors and how these brain...

  9. The cortical organization of audio-visual sentence comprehension: an fMRI study at 4 Tesla

    E-Print Network [OSTI]

    The cortical organization of audio-visual sentence comprehension: an fMRI study at 4 Tesla Cheryl M Tesla. Participants viewed the face and upper body of a speaker via a video screen while listening

  10. Cortical Plasticity and Behavioral Recovery Following Focal Lesion to Primary Motor Cortex in Adult Rats

    E-Print Network [OSTI]

    Nishibe, Mariko

    2012-05-31

    ………………………………..…………42 Fig 10 Anatomical substrate for cortical motor map competition: convergence of corticospinal projections of M1………………………………………………………………………….……..43 Fig 11 Single-pellet reach and retrieval task………………………………………….………….44 CHAPTER TWO Fig 1... in inhibiting the inaccurate placing response (Barth et al., 1990). The M1/S1 rough mirror-image connectivity particularly plays a crucial role in the movements presumably necessary for single-pellet reach and retrieval task. Rats first locate a singly...

  11. Bone scintigraphy in evaluating the viability of composite bone grafts revascularized by microvascular anastomoses, conventional autogenous bone grafts, and free non-revascularized periosteal grafts

    SciTech Connect (OSTI)

    Berggren, A.; Weiland, A.J.; Ostrup, L.T.

    1982-07-01

    Researchers studied the value of bone scintigraphy in the assessment of anastomotic patency and bone-cell viability in free bone grafts revascularized by microvascular anastomoses in twenty-seven dogs. The dogs were divided into three different groups, and scintigraphy was carried out using technetium-labeled methylene diphosphonate in composite bone grafts revascularized by microvascular anastomoses, conventional autogenous bone grafts, and periosteal grafts placed in different recipient beds. The viability of the grafts were evaluated by histological examination and fluorescence microscopy after triple labeling with oxytetracycline on the first postoperative day, alizarin complexone on the fourth postoperative day, and DCAF on the eleventh postoperative day. A positive scintiscan within the first week following surgery indicated patent microvascular anastomoses, and histological study and fluorescence microscopy confirmed that bone throughout the graft was viable. A positive scintiscan one week after surgery or later does not necessarily indicate microvascular patency or bone-cell survival, because new bone formed by creeping substitution on the surface of a dead bone graft can result in this finding.

  12. Modeling aspects of human memory for scientific study.

    SciTech Connect (OSTI)

    Caudell, Thomas P. (University of New Mexico); Watson, Patrick (University of Illinois - Champaign-Urbana Beckman Institute); McDaniel, Mark A. (Washington University); Eichenbaum, Howard B. (Boston University); Cohen, Neal J. (University of Illinois - Champaign-Urbana Beckman Institute); Vineyard, Craig Michael; Taylor, Shawn Ellis; Bernard, Michael Lewis; Morrow, James Dan; Verzi, Stephen J.

    2009-10-01

    Working with leading experts in the field of cognitive neuroscience and computational intelligence, SNL has developed a computational architecture that represents neurocognitive mechanisms associated with how humans remember experiences in their past. The architecture represents how knowledge is organized and updated through information from individual experiences (episodes) via the cortical-hippocampal declarative memory system. We compared the simulated behavioral characteristics with those of humans measured under well established experimental standards, controlling for unmodeled aspects of human processing, such as perception. We used this knowledge to create robust simulations of & human memory behaviors that should help move the scientific community closer to understanding how humans remember information. These behaviors were experimentally validated against actual human subjects, which was published. An important outcome of the validation process will be the joining of specific experimental testing procedures from the field of neuroscience with computational representations from the field of cognitive modeling and simulation.

  13. Journal of Biomechanics 40 (2007) 36883699 Reliable simulations of the human proximal femur by high-order finite

    E-Print Network [OSTI]

    Yosibash, Zohar

    2007-01-01

    Journal of Biomechanics 40 (2007) 3688­3699 Reliable simulations of the human proximal femur to in-vitro experiments. ASME Journal of Biomechanical Engineering 129(3), 297­309.] for the reliable; Bone biomechanics 1. Introduction The mechanical response of an individual patient's bone

  14. New Insights into the Evolution of the Human Diet from Faecal Biomarker Analysis in Wild Chimpanzee and Gorilla Faeces

    E-Print Network [OSTI]

    Wrangham, Richard

    Our understanding of early human diets is based on reconstructed biomechanics of hominin jaws, bone and teeth isotopic data, tooth wear patterns, lithic, taphonomic and zooarchaeological data, which do not provide information ...

  15. Bone Cancer Rates in Dinosaurs Compared with Modern Vertebrates

    E-Print Network [OSTI]

    L. C. Natarajan; A. L. Melott; B. M. Rothschild; L. D. Martin

    2007-10-16

    Data on the prevalence of bone cancer in dinosaurs is available from past radiological examination of preserved bones. We statistically test this data for consistency with rates extrapolated from information on bone cancer in modern vertebrates, and find that there is no evidence of a different rate. Thus, this test provides no support for a possible role of ionizing radiation in the K-T extinction event.

  16. Human energy

    E-Print Network [OSTI]

    Sawyer, Suzana

    2010-01-01

    This is the power of human energy that Chevron neverExperience the power of ‘‘Human Energy’’: S. Sawyer (&)s voice returns: ‘‘This is the power of human energy. ’’ In

  17. Physiological Stress, Bone Growth and Development in Imperial Rome

    E-Print Network [OSTI]

    Beauchesne, Patrick Denis

    2012-01-01

    present and that diagenesis (chemical exchange between therisk assessment. Diagenesis, or the chemical exchangeto assess the level of diagenesis in a bone without chemical

  18. Investigation into mechanical properties of bone and its main constituents

    E-Print Network [OSTI]

    Evdokimenko, Ekaterina

    2012-01-01

    mineral to the elastic-plastic properties of bone. J Bonec properties, hardness, viscosity and plastic deformation ofin the plastic region is a function of collagen properties

  19. Trabecular bone dosimetry using a Monte Carlo code 

    E-Print Network [OSTI]

    Zuzarte de Mendonca, Anne

    1993-01-01

    thickness 24 7. Example of the path of a volume-seeker in trabecular bone using EGS4. S is the source point 28 8. Absorbed &actions in the bone marmw in case of a uniform deposition in trabecular bone. CS in mm and f are indicated in parenthesis for each... percent per mass) 29 4. Electron absorbed fractions and COV (%) for monoenergetic electron sources uniformly distributed within the trabecular bone of the middle spine (CS&. 99 mm and f=1. 012) 31 5. Electmn absorbed fractions and COV...

  20. CX3CR1 Is Expressed by Prostate Epithelial Cells and Androgens Regulate the Levels of CX3CL1/Fractalkine in the Bone Marrow

    E-Print Network [OSTI]

    Fatatis, Alessandro

    CX3CR1 Is Expressed by Prostate Epithelial Cells and Androgens Regulate the Levels of CX3CL1 human osteoblasts in vitro. Thus, the interaction of fractalkine with its receptor CX3CR1 could play a crucial role in vivo by directing circulating prostate cancer cells to the bone. We found that although CX

  1. Analysis of the Heritability of Cortical Inversions through Sexual Exchange in Paramecium tetraurelia 

    E-Print Network [OSTI]

    Cross, Rebecca

    2012-04-23

    , the trichocyst is a membrane-bounded organelle that looks like a “carrot with a golf tee” attached to the end, and is 5 ?m long. They are associated with the plasma membrane at insertion or docking sites on the anterior-posterior boundaries between cortical...” is converted into a 25 ?m spear-like shape (Pollack 1974). About 40 or 50 Mendelian loci have been identified which contribute to trichocyst assembly or discharge at various developmental stages. One such mutation, nd6, results in fully assembled...

  2. Involvement of ERK in NMDA receptor-independent cortical neurotoxicity of hydrogen sulfide

    SciTech Connect (OSTI)

    Kurokawa, Yuko; Sekiguchi, Fumiko; Kubo, Satoko; Yamasaki, Yoshiko; Matsuda, Sachi; Okamoto, Yukari; Sekimoto, Teruki; Fukatsu, Anna; Nishikawa, Hiroyuki [Division of Pharmacology and Pathophysiology, Kinki University School of Pharmacy, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan)] [Division of Pharmacology and Pathophysiology, Kinki University School of Pharmacy, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan); Kume, Toshiaki [Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501 (Japan)] [Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501 (Japan); Fukushima, Nobuyuki [Division of Molecular Neurobiology, Department of Life Sciences, Kinki University School of Science and Engineering, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan)] [Division of Molecular Neurobiology, Department of Life Sciences, Kinki University School of Science and Engineering, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan); Akaike, Akinori [Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501 (Japan)] [Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501 (Japan); Kawabata, Atsufumi, E-mail: kawabata@phar.kindai.ac.jp [Division of Pharmacology and Pathophysiology, Kinki University School of Pharmacy, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan)] [Division of Pharmacology and Pathophysiology, Kinki University School of Pharmacy, 3-4-1 Kowakae, Higashi-Osaka 577-8502 (Japan)

    2011-11-04

    Highlights: Black-Right-Pointing-Pointer Hydrogen sulfide causes NMDA receptor-independent neurotoxicity in mouse fetal cortical neurons. Black-Right-Pointing-Pointer Activation of ERK mediates the toxicity of hydrogen sulfide. Black-Right-Pointing-Pointer Apoptotic mechanisms are involved in the hydrogen-induced cell death. -- Abstract: Hydrogen sulfide (H{sub 2}S), a gasotransmitter, exerts both neurotoxicity and neuroprotection, and targets multiple molecules including NMDA receptors, T-type calcium channels and NO synthase (NOS) that might affect neuronal viability. Here, we determined and characterized effects of NaHS, an H{sub 2}S donor, on cell viability in the primary cultures of mouse fetal cortical neurons. NaHS caused neuronal death, as assessed by LDH release and trypan blue staining, but did not significantly reduce the glutamate toxicity. The neurotoxicity of NaHS was resistant to inhibitors of NMDA receptors, T-type calcium channels and NOS, and was blocked by inhibitors of MEK, but not JNK, p38 MAP kinase, PKC and Src. NaHS caused prompt phosphorylation of ERK and upregulation of Bad, followed by translocation of Bax to mitochondria and release of mitochondrial cytochrome c, leading to the nuclear condensation/fragmentation. These effects of NaHS were suppressed by the MEK inhibitor. Our data suggest that the NMDA receptor-independent neurotoxicity of H{sub 2}S involves activation of the MEK/ERK pathway and some apoptotic mechanisms.

  3. Microcapsule-Induced Toughening of Bone Cement Gina M. Miller

    E-Print Network [OSTI]

    Sottos, Nancy R.

    27 Microcapsule-Induced Toughening of Bone Cement Gina M. Miller Senior in Aerospace Engineering R. White, and TAM Prof. Nancy R. Sottos Acrylic bone cement is the primary material used cement, it may be possible to extend the lifetime of the implant, thus reducing the occurrence

  4. Bone loss during energy restriction: mechanistic role of leptin 

    E-Print Network [OSTI]

    Baek, Kyunghwa

    2009-05-15

    Mechanical unloading and food restriction (FR) are leading causes of bone loss, which increase the risk of fracture later in life. Leptin, a 16kDa cytokine like hormone principally produced by white adipocytes, may be involved in bone metabolism...

  5. p-FEMs in biomechanics: Bones and Arteries Zohar Yosibash

    E-Print Network [OSTI]

    Yosibash, Zohar

    p-FEMs in biomechanics: Bones and Arteries Zohar Yosibash Department of Mechanical Engineering, Ben-FEM) is extended to problems in the field of biomechanics: the mechanical response of bones and arteries-FEMs are extremely important for analysts that aim at validating mathe- matical models of biomechanical structures

  6. Protocadherin-7 induces bone metastasis of breast cancer

    SciTech Connect (OSTI)

    Li, Ai-Min; Tian, Ai-Xian; Zhang, Rui-Xue; Ge, Jie; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Sun, Xuan; Cao, Xu-Chen; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin

    2013-07-05

    Highlights: •PCDH7 is overexpression in high bone metastatic MDA-MB-231 cells. •PCDH7 is up-regulation in bone metastatic breast cancer tissues. •Suppression of PCDH7 inhibits cell proliferation, migration, and invasion in vitro. •PCDH7 induces breast cancer bone metastasis in vivo. -- Abstract: Breast cancer had a propensity to metastasize to bone, resulting in serious skeletal complications associated with poor outcome. Previous study showed that Protocadherin-7 (PCDH7) play an important role in brain metastatic breast cancer, however, the role of PCDH7 in bone metastatic breast cancer has never been explored. In the present study, we found that PCDH7 expression was up-regulation in bone metastatic breast cancer tissues by real-time PCR and immunohistochemistry assays. Furthermore, suppression of PCDH7 inhibits breast cancer cell proliferation, migration, and invasion in vitro by MTT, scratch, and transwell assays. Most importantly, overexpression of PCDH7 promotes breast cancer cell proliferation and invasion in vitro, and formation of bone metastasis in vivo. These data provide an important insight into the role of PCDH7 in bone metastasis of breast cancer.

  7. Drilling and Microfracture Lead to Different Bone Structure and Necrosis during Bone-Marrow Stimulation for Cartilage Repair

    E-Print Network [OSTI]

    Buschmann, Michael

    Drilling and Microfracture Lead to Different Bone Structure and Necrosis during Bone following microfracture and comparing to drilling in a mature rabbit model of cartilage repair. Microfracture holes were made to a depth of 2 mm and drill holes to either 2 mm or 6 mm under cooled irrigation

  8. A Novel Inverse Finite Element Analysis to Assess Bone Fracture Healing in Mice Receiving Bone Marrow Mesenchymal Stem Cell Transplantation

    E-Print Network [OSTI]

    Miga, Michael I.

    A Novel Inverse Finite Element Analysis to Assess Bone Fracture Healing in Mice Receiving Bone generation, and an iterative optimization (using finite element analysis) of the fracture callus material approach includes acquisition of microCT image volumes, biomechanical testing, finite element mesh

  9. Autism: Reduced Connectivity between Cortical Areas Involved in Face Expression, Theory of Mind, and the Sense of Self

    E-Print Network [OSTI]

    Feng, Jianfeng

    1 Autism: Reduced Connectivity between Cortical Areas Involved in Face Expression, Theory of Mind head: Functional connectivity in autism # These authors contributed equally to this work resting-state functional connectivity was analyzed in 523 people with autism and 452 typically developing

  10. Automatic segmentation of cortical vessels in pre-and post-tumor resection laser range scan images

    E-Print Network [OSTI]

    Miga, Michael I.

    Automatic segmentation of cortical vessels in pre- and post- tumor resection laser range scan have developed a method, which permits this registration using vessels visible in the images. But, vessel segmentation required the localization of starting and ending points for each vessel segment. Here

  11. Osmotic Stress Regulates Mineralocorticoid Receptor Expression in a Novel Aldosterone-Sensitive Cortical Collecting Duct Cell Line

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 Osmotic Stress Regulates Mineralocorticoid Receptor Expression in a Novel Aldosterone 49 59 67 32; E-mail: marc.lombes@u-psud.fr Abbreviated Title: Osmotic Stress and MR Expression Precis: Osmotic Stress Regulates Mineralocorticoid Receptor Expression in Cortical Collecting Duct Cells

  12. Anatomy of the visual word form area: Adjacent cortical circuits and long-range white matter connections

    E-Print Network [OSTI]

    Wandell, Brian A.

    Anatomy of the visual word form area: Adjacent cortical circuits and long-range white matter, United States a r t i c l e i n f o Article history: Available online xxxx Keywords: Visual word form t Circuitry in ventral occipital-temporal cortex is essential for seeing words. We analyze the circuitry

  13. V. Cortical Development and Neural Disorder Fates and functions of NG2 cells in the postnatal CNS

    E-Print Network [OSTI]

    Kazama, Hokto

    V. Cortical Development and Neural Disorder Fates and functions of NG2 cells in the postnatal CNS: Oligodendrocyte precursors (OLPs, also known as NG2 cells) are generated in the ventricular zones (VZ neurons at earlier developmental times. NG2 cells subsequently proliferate and migrate widely throughout

  14. DISCOVERY AND RESEARCH ON JIAHU BONE FLUTES IN WUYANG, CHINA.

    SciTech Connect (OSTI)

    JUZHONG, Z.; HARBOTTLE, G.; XINGHUA, X.; CHANGSUI, W.

    2000-11-01

    The site of Jiahu is located in Jiahu village, Wuyang County, Henan province, on the Western edge of the broad plain of Huanhuaihai. On its north the site borders the Sha River, in the upper reaches of the Huai River; its latitude is 33{degree} 36 minutes North, longitude 113{degree} 40 minutes East, and it is 67.5 meters above sea level. Between 1983 and 1987, the Henan Cultural Relics and Archaeology Institute carried out six campaigns of excavation here, revealing an area of 2400 square meters. Except for the trial excavation in the spring of 1983, Zhang Juzhong has been in charge of all the excavations. In early May 1986, while excavating tomb 78, Zhang Juzhong and Yang Zhenwei first discovered two funerary bone flutes. They soon found other, similar bone flutes in tombs 73, 94 and 121 respectively. Mr. Zhang's attention was instantly focused on these remarkable finds. In the campaign of autumn 1986, one or two more bone flutes were discovered in each of tombs 233,273, 263 and 270. Finally, in the spring of 1987, again one or two bone flutes were found in each of the tombs 282, 363,341,411,344 and 387. Up to the end of excavation in June 1987, altogether 25 bone flutes had been found, of which 17 were complete or almost complete, 6 broken or fragmentary and 2 were half-finished examples. Among the 17 complete bone flutes, there were 14 having seven holes, one five-hole, one six-hole and one eight-hole bone flute. In particular, the bone flute M282:20 was exquisitely made, and complete. Zhang Juzhong, the discoverer of the bone flutes, researcher Pei Mingxiang, the. ex-director of the division, who came to the digging site to see the progress of the work, and their coworkers were all understandably very excited.

  15. Desynchronizing effect of high-frequency stimulation in a generic cortical network model

    E-Print Network [OSTI]

    Markus Schütt; Jens Christian Claussen

    2012-03-21

    Transcranial Electrical Stimulation (TCES) and Deep Brain Stimulation (DBS) are two different applications of electrical current to the brain used in different areas of medicine. Both have a similar frequency dependence of their efficiency, with the most pronounced effects around 100Hz. We apply superthreshold electrical stimulation, specifically depolarizing DC current, interrupted at different frequencies, to a simple model of a population of cortical neurons which uses phenomenological descriptions of neurons by Izhikevich and synaptic connections on a similar level of sophistication. With this model, we are able to reproduce the optimal desynchronization around 100Hz, as well as to predict the full frequency dependence of the efficiency of desynchronization, and thereby to give a possible explanation for the action mechanism of TCES.

  16. Processing of hydroxylapatite coatings on titanium alloy bone prostheses

    DOE Patents [OSTI]

    Nastasi, Michael A. (Espanola, NM); Levine, Timothy E. (Santa Clara, CA); Mayer, James W. (Phoenix, AZ); Pizziconi, Vincent B. (Phoenix, AZ)

    1998-01-01

    Processing of hydroxylapatite sol-gel films on titanium alloy bone prostheses. A method utilizing non-line-of-sight ion beam implantation and/or rapid thermal processing to provide improved bonding of layers of hydroxylapatite to titanium alloy substrates while encouraging bone ingrowth into the hydroxylapatite layers located away from the substrate, is described for the fabrication of prostheses. The first layer of hydroxylapatite is mixed into the substrate by the ions or rapidly thermally annealed, while subsequent layers are heat treated or densified using ion implantation to form layers of decreasing density and larger crystallization, with the outermost layers being suitable for bone ingrowth.

  17. JOURNAL OF BONE AND MINERAL RESEARCH Volume 24, Number 11, 2009

    E-Print Network [OSTI]

    Burgoyne, Chris

    JOURNAL OF BONE AND MINERAL RESEARCH Volume 24, Number 11, 2009 Published online on May 4, 2009; doi: 10.1359/JBMR.090504 Ó 2009 American Society for Bone and Mineral Research Femoral Neck Trabecular fracture cases have sufficient trabecular bone for anabolic therapies to build on. J Bone Miner Res 2009;24:1808

  18. ORIGINAL RESEARCH Minerals Form a Continuum Phase in Mature Cancellous Bone

    E-Print Network [OSTI]

    Price, Paul A.

    of fully deprotei- nized and demineralized bovine femur cancellous bone samples show that fine trabecular

  19. An experimental study of diffusional properties of small ions and nonelectrolytes in compact bone 

    E-Print Network [OSTI]

    Bilge, Huseyin Fertac

    1978-01-01

    structure have been reviewed by Richeile and Onkelinx [43] who discuss the composition, structure and the dynamics of bone mineral. Bauer, et al. [2] have presented the homeostatic function of bone in relation to bone metabolism. They have shown... functions, it has been recog- nized for many years that bone tissue has important physiological func- tions relating to whole body metabolism and that its microscopic struc- ture is equally well adapted to these functions. It is well known that bone...

  20. Compact biomedical pulsed signal generator for bone tissue stimulation

    DOE Patents [OSTI]

    Kronberg, J.W.

    1993-06-08

    An apparatus for stimulating bone tissue for stimulating bone growth or treating osteoporosis by applying directly to the skin of the patient an alternating current electrical signal comprising wave forms known to simulate the piezoelectric constituents in bone. The apparatus may, by moving a switch, stimulate bone growth or treat osteoporosis, as desired. Based on low-power CMOS technology and enclosed in a moisture-resistant case shaped to fit comfortably, two astable multivibrators produce the desired waveforms. The amplitude, pulse width and pulse frequency, and the subpulse width and subpulse frequency of the waveforms are adjustable. The apparatus, preferably powered by a standard 9-volt battery, includes signal amplitude sensors and warning signals indicate an output is being produced and the battery needs to be replaced.

  1. Compact biomedical pulsed signal generator for bone tissue stimulation

    DOE Patents [OSTI]

    Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

    1993-01-01

    An apparatus for stimulating bone tissue for stimulating bone growth or treating osteoporosis by applying directly to the skin of the patient an alternating current electrical signal comprising wave forms known to simulate the piezoelectric constituents in bone. The apparatus may, by moving a switch, stimulate bone growth or treat osteoporosis, as desired. Based on low-power CMOS technology and enclosed in a moisture-resistant case shaped to fit comfortably, two astable multivibrators produce the desired waveforms. The amplitude, pulse width and pulse frequency, and the subpulse width and subpulse frequency of the waveforms are adjustable. The apparatus, preferably powered by a standard 9-volt battery, includes signal amplitude sensors and warning signals indicate an output is being produced and the battery needs to be replaced.

  2. Modular ‘Click-in-Emulsion’ Bone-Targeted Nanogels

    E-Print Network [OSTI]

    Heller, Daniel A.

    A new class of nanogel demonstrates modular biodistribution and affinity for bone. Nanogels, ~70 nm in diameter and synthesized via an astoichiometric click-chemistry in-emulsion method, controllably display residual, free ...

  3. On the mechanistic origins of toughness in bone

    SciTech Connect (OSTI)

    Launey, Maximilien E.; Buehler, Markus J.; Ritchie, Robert O.

    2009-10-07

    One of the most intriguing protein materials found in Nature is bone, a material composed out of assemblies of tropocollagen molecules and tiny hydroxyapatite mineral crystals, forming an extremely tough, yet lightweight, adaptive and multi-functional material. Bone has evolved to provide structural support to organisms, and therefore, its mechanical properties are of great physiological relevance. In this article, we review the structure and properties of bone, focusing on mechanical deformation and fracture behavior from the perspective of the multi-dimensional hierarchical nature of its structure. In fact, bone derives its resistance to fracture with a multitude of deformation and toughening mechanisms at many of these size-scales, ranging from the nanoscale structure of its protein molecules to its macroscopic physiological scale.

  4. Research Finds Vitamin D Deficiency Affects Bone Quality

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the ALS has also found that vitamin D deficiency plays a significant role in the bone-aging process. Low levels of vitamin D, the "sunshine vitamin," have been previously linked...

  5. The bones of the Milky Way

    SciTech Connect (OSTI)

    Goodman, Alyssa A.; Beaumont, Christopher N. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Alves, João [University of Vienna, 1180 Vienna (Austria); Benjamin, Robert A. [University of Wisconsin-Whitewater, Whitewater, WI 53190 (United States); Borkin, Michelle A. [Harvard University, Cambridge, MA 02138 (United States); Burkert, Andreas [University of Munich, Munich (Germany); Dame, Thomas M. [Smithsonian Astrophysical Observatory, Cambridge, MA 02138 (United States); Jackson, James [Boston University, Boston, MA 02215 (United States); Kauffmann, Jens [California Institute of Technology, Pasadena, CA 91125 (United States); Robitaille, Thomas [Max Planck Institute for Astronomy, Heidelberg (Germany); Smith, Rowan J. [Institut für Theoretische Astrophysik, Zentrum für Astronomie der Universiät Heidelberg, Heidelberg (Germany)

    2014-12-10

    The very long and thin infrared dark cloud 'Nessie' is even longer than had been previously claimed, and an analysis of its Galactic location suggests that it lies directly in the Milky Way's mid-plane, tracing out a highly elongated bone-like feature within the prominent Scutum-Centaurus spiral arm. Re-analysis of mid-infrared imagery from the Spitzer Space Telescope shows that this infrared dark cloud (IRDC) is at least two and possibly as many as five times longer than had originally been claimed by Nessie's discoverers; its aspect ratio is therefore at least 300:1 and possibly as large as 800:1. A careful accounting for both the Sun's offset from the Galactic plane (?25 pc) and the Galactic center's offset from the (l{sup II} , b{sup II} ) = (0, 0) position shows that the latitude of the true Galactic mid-plane at the 3.1 kpc distance to the Scutum-Centaurus Arm is not b = 0, but instead closer to b = –0.4, which is the latitude of Nessie to within a few parsecs. An analysis of the radial velocities of low-density (CO) and high-density (NH{sub 3}) gas associated with the Nessie dust feature suggests that Nessie runs along the Scutum-Centaurus Arm in position-position-velocity space, which means it likely forms a dense 'spine' of the arm in real space as well. The Scutum-Centaurus Arm is the closest major spiral arm to the Sun toward the inner Galaxy, and, at the longitude of Nessie, it is almost perpendicular to our line of sight, making Nessie the easiest feature to see as a shadow elongated along the Galactic plane from our location. Future high-resolution dust mapping and molecular line observations of the harder-to-find Galactic 'bones' should allow us to exploit the Sun's position above the plane to gain a (very foreshortened) view 'from above' the Milky Way's structure.

  6. Ibuprofen Administered Pre- or Post- Simulated Resistance Exercise Training Does Not Diminsh Gains in Bone Formation or Bone Mass 

    E-Print Network [OSTI]

    Cunningham, David

    2012-02-14

    Non-steroidal anti-inflammatory drugs (NSAIDs) have been shown to suppress bone formation when administered before, but not if administered after, an acute bout of mechanical load in rats. The NSAID ibuprofen inhibits ...

  7. Noninvasive and quantitative evaluation of bone marrow infiltration and bone marrow microcirculation in patients with monoclonal plasma cell disease by means of diffusion

    E-Print Network [OSTI]

    Gertz, Michael

    .0001) and D (p study clearly shows that bone marrow infiltrationRajiv Shah Dr. med. Noninvasive and quantitative evaluation of bone marrow infiltration and bone diseases. The response to treatment is evaluated mostly on the basis of hematologic parameters. This study

  8. On-off intermittency of thalamo-cortical oscillations in the electroencephalogram of rats with genetic predisposition to absence epilepsy

    E-Print Network [OSTI]

    Evgenia Sitnikova; Alexander E. Hramov; Alexey A. Ovchinnikov; Alexey A. Koronovskii

    2013-02-17

    Spike-wave discharges (SWD) are electroencephalographic hallmarks of absence epilepsy. SWD are known to originate from thalamo-cortical neuronal network that normally produce sleep spindle oscillations. Although both sleep spindles and SWD are considered as thalamo-cortical oscillations, functional relationship between them is still uncertain. The present study describes temporal dynamics of SWD and sleep spindles as determined in long-term EEG recordings in WAG/Rij rat model of absence epilepsy. It was found that non-linear dynamics of SWD fits well to the law of 'on-off intermittency'. Typical sleep spindles that occur during slow-wave sleep (SWS) also demonstrated 'on-off intermittency' behavior, in contrast to high-voltage spindles during intermediate sleep stage, whose dynamics was uncertain. This implies that both SWS sleep spindles and SWD are controlled by a system-level mechanism that is responsible for regulating circadian activity and/or sleep-wake transitions.

  9. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 679 (2015) | doi:10.1038/bonekey.2015.46

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number `Defining the Bone Metastasis Niche'. CABS ORAL COMMUNICATIONS The 4th Joint Meeting of ECTS and IBMS

  10. Mechanism of dynamic reorientation of cortical microtubules due to mechanical stress

    E-Print Network [OSTI]

    Alexander Muratov; Vladimir A. Baulin

    2015-10-13

    Directional growth caused by gravitropism and corresponding bending of plant cells has been explored since 19th century, however, many aspects of mechanisms underlying the perception of gravity at the molecular level are still not well known. Perception of gravity in root and shoot gravitropisms is usually attributed to gravisensitive cells, called statocytes, which exploit sedimentation of macroscopic and heavy organelles, amyloplasts, to sense the direction of gravity. Gravity stimulus is then transduced into distal elongation zone, which is several mm far from statocytes, where it causes stretching. It is suggested that gravity stimulus is conveyed by gradients in auxin flux. We propose a theoretical model that may explain how concentration gradients and/or stretching may indirectly affect the global orientation of cortical microtubules, attached to the cell membrane and induce their dynamic reorientation perpendicular to the gradients. In turn, oriented microtubules arrays direct the growth and orientation of cellulose microfibrils, forming part of the cell external skeleton and determine the shape of the cell. Reorientation of microtubules is also observed in reaction to light in phototropism and mechanical bending, thus suggesting universality of the proposed mechanism.

  11. Common variants in the region around Osterix are associated with bone mineral density and growth in childhood

    E-Print Network [OSTI]

    Peltonen, Leena

    Peak bone mass achieved in adolescence is a determinant of bone mass in later life. In order to identify genetic variants affecting bone mineral density (BMD), we performed a genome-wide association study of BMD and related ...

  12. Automated simulation of areal bone mineral density assessment in the distal radius from high-resolution peripheral quantitative computed tomography

    E-Print Network [OSTI]

    Burghardt, A. J.; Kazakia, G. J.; Link, T. M.; Majumdar, S.

    2009-01-01

    of osteoporotic fractures. J Bone Miner Res 16:1108–1119 3.of Osteoporotic Fractures. J Bone Miner Res 18:1947–1954 4.and calcaneus. J Bone Miner Res 14:1167–1174 7. Muller R,

  13. Engineered [beta]TCP-binding HER-family protein fusions and their use for improving osteoprogenitor- mediated bone regeneration

    E-Print Network [OSTI]

    Rivera Abreu, Jaime J. (Jaime Jose)

    2015-01-01

    Autologous bone marrow grafting has been shown to aid in the healing of bone defects since the 1950s. Transplantation of freshly-aspirated autologous bone marrow, together with a scaffold, is a promising clinical alternative ...

  14. PDGFBB promotes PDGFR{alpha}-positive cell migration into artificial bone in vivo

    SciTech Connect (OSTI)

    Yoshida, Shigeyuki; Center for Human Metabolomic Systems Biology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Iwasaki, Ryotaro; Kawana, Hiromasa; Miyauchi, Yoshiteru; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582; Department of Integrated Bone Metabolism and Immunology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Hoshi, Hiroko; Miyamoto, Hiroya; Mori, Tomoaki; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Kanagawa, Hiroya; Katsuyama, Eri; Fujie, Atsuhiro; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582 ; Hao, Wu; and others

    2012-05-18

    Highlights: Black-Right-Pointing-Pointer We examined effects of PDGFBB in PDGFR{alpha} positive cell migration in artificial bones. Black-Right-Pointing-Pointer PDGFBB was not expressed in osteoblastic cells but was expressed in peripheral blood cells. Black-Right-Pointing-Pointer PDGFBB promoted PDGFR{alpha} positive cell migration into artificial bones but not osteoblast proliferation. Black-Right-Pointing-Pointer PDGFBB did not inhibit osteoblastogenesis. -- Abstract: Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor {alpha} (PDGFR{alpha})-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone in vivo. Growth factors such as transforming growth factor beta (TGF{beta}) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.

  15. Compressive behavior of trabecular bone in the proximal tibia using a cellular solid model 

    E-Print Network [OSTI]

    Prommin, Danu

    2005-11-01

    In this study, trabecular architecture is considered as a cellular solid structure, including both intact and damaged bone models. ??Intact?? bone models were constructed based on ideal versions of 25, 60 and 80-year-old ...

  16. Bone Tissue Engineering Using Colloidal Gels and Native Extracellular Matrix Biomaterials

    E-Print Network [OSTI]

    Dennis, Stephen Connor

    2015-05-31

    scaffolding technology for the regeneration of bone tissue in non-load bearing critical-sized defects. This represents the first attempt to form colloids exclusively from biomaterials found in the microenvironment of healing bone fractures including hyaluronic...

  17. Bone Canonical WNT/B-Catenin Signaling in Models of Reduced Microgravity 

    E-Print Network [OSTI]

    Macias, Brandon 1979-

    2012-10-25

    translates into molecular osteogenic signals in bone cells is unknown. Radiation exposure is another potent inducer of bone loss, namely observed on Earth in the clinical setting following radiotherapy procedures. It is expected that long duration space...

  18. Apatite-polymer composites for the controlled delivery of bone morphogenetic proteins

    E-Print Network [OSTI]

    Yong, Tseh-Hwan

    2005-01-01

    Current treatment of bone defects due to trauma, cancer, or degenerative spine diseases involves the implantation of a bone graft. Autografts, which are harvested from the patient's own body, are associated with problems ...

  19. Analysis and design of an adjustable bone plate for mandibular fracture fixation

    E-Print Network [OSTI]

    Cervantes, Thomas Michael

    2011-01-01

    This thesis presents the design, analysis and testing of a bone plate for mandibular fracture fixation. Conventional bone plates are commonly used to set fractures of the mandible in a surgical setting. If proper alignment ...

  20. Impact of Omega-3 Polyunsaturated Fatty Acids on Bone Adaptations to Simulated Resistance Training 

    E-Print Network [OSTI]

    Camp, Kaleigh Ann

    2013-04-30

    Young and ovariectomized animals eating diets rich in omega-3 polyunsaturated fatty acids (n-3 PUFAs) exhibit enhanced bone formation and decrease bone loss, respectively. Eicosapentaenoic acid, an n-3 PUFA found in fish ...

  1. Detection of bone disease in dogs by radioisotope scanning 

    E-Print Network [OSTI]

    Morris, Earl Louis

    1971-01-01

    in dogs for the diagnosis and progression of bone disease was studied. Twc isotopes of strontium, Sr and 85 87m Sr, were studied. The Sr was purchased 85 commercially and the Sr was produced at the 87m Texas A8cM University Nuclear Science Center... by the irradiation oi' Sr(N05)2. Criteria for bone scanning in dogs using Sr 85 and Sr were determined. Pour normal young dogs 87m were injected with Sr and four with Sr. Doses 85 87m of 100 p&i of Sr and 1 mCi of Sr were found . to 85 87m produoe satisfactory...

  2. Measurement of bone mineral content in caged and active cats 

    E-Print Network [OSTI]

    Tveter, Diane Ellen

    1988-01-01

    errors but these can be reduced by using two different x-ray energies. Dual energy CT operates on a basis similar to dual photon absorptiometry (explained below). The difference in attenuation between tissue and bone is greater for a lower energy... to act as a soft tissue equivalent (35). Effects of fat and soft tissue are decreased when dual energy CT is used (33). Data from each of the two different photon energies are combined and result in images of soft tissue and bone mineral regions. Beam...

  3. Is decreased bone mineral density associated with development of scoliosis? A bipedal osteopenic rat model

    E-Print Network [OSTI]

    Dede, Ozgur; Akel, Ibrahim; Demirkiran, Gokhan; Yalcin, Nadir; Marcucio, Ralph; Acaroglu, Emre

    2011-01-01

    more time standing erect. Dual energy X-ray absorbtiometry (acid; DEXA: Dual energy X-ray absorptiometry; BMD: Bone

  4. Antler Stiffness in Moose (Alces alces): Correlated Evolution of Bone Function and Material Properties?

    E-Print Network [OSTI]

    Blob, Richard W.

    , Clemson, South Carolina 29634 ABSTRACT The material properties of bone can vary considerably among in bone material properties might be correlated with differing functional demands at any of these scales- tudes like the Yukon has the potential to compromise bone material properties. However, moose have

  5. Flexible Statistical Models for Growth Fragments: a Study of Bone Mineral Acquisition

    E-Print Network [OSTI]

    Hastie, Trevor

    Flexible Statistical Models for Growth Fragments: a Study of Bone Mineral Acquisition Trevor J describe some statistical modeling of longitudinal data from a pediatric study of bone mineral acquisition- surements of bone mineral density are available for each subject, taken roughly one year apart, and between

  6. JOURNAL OF BONE AND MINERAL RESEARCH Volume 24, Number 2, 2009

    E-Print Network [OSTI]

    Ritchie, Robert

    JOURNAL OF BONE AND MINERAL RESEARCH Volume 24, Number 2, 2009 Published online on October 13, 2008; doi: 10.1359/JBMR.81005 Ó 2009 American Society for Bone and Mineral Research Prolonged Treatments With Antiresorptive Agents and PTH Have Different Effects on Bone Strength and the Degree of Mineralization in Old

  7. Donor-Derived, Liver-Specific Protein Expression after Bone Marrow Transplantation

    E-Print Network [OSTI]

    Kay, Mark A.

    Donor-Derived, Liver-Specific Protein Expression after Bone Marrow Transplantation D. Denison mechanism to deliver a functional gene to a deficient liver. Bone marrow-derived hepatocytes are rare to treat liver disease is unclear. We sought to quantify bone marrow-derived hepatocyte protein expression

  8. Remembering the past : multimodal imaging of cortical contributions to episodic retrieval

    E-Print Network [OSTI]

    Kahn, Itamar

    2005-01-01

    What is the nature of the neural processes that allow humans to remember past events? The theoretical framework adopted in this thesis builds upon cognitive models that suggest that episodic retrieval can be decomposed ...

  9. Refinement of the $n-?$ and $p-?$ fish-bone potential

    E-Print Network [OSTI]

    E. Smith; R. Woodhouse; Z. Papp

    2012-09-04

    The fishbone potential of composite particles simulates the Pauli effect by nonlocal terms. We determine the $n-\\alpha$ and $p-\\alpha$ fish-bone potential by simultaneously fitting to the experimental phase shifts. We found that with a double Gaussian parametrization of the local potential can describe the $n-\\alpha$ and $p-\\alpha$ phase shifts for all partial waves.

  10. GEORGIA TECHEMORYPKU SEED GRANT PROGRAM Nanostructure at Bone

    E-Print Network [OSTI]

    Das, Suman

    nanofibers to mimic the collagen fiber structure of bone matrix. #12;· Electrospinning is a simple and natural polymers. Electrospinning #12;PMMA PEO #12;Alginate fiber SEM image of the alginate in THF/DMF solvent Fabricate the fibrous scaffolds by electrospinning Calcine the samples to remove

  11. Scaffolds for bone repair using computer aided design and manufacture 

    E-Print Network [OSTI]

    Vadillo, Philippe Tadeusz

    2009-01-01

    of the extruder, the diameter of the pores can be precisely controlled to an accuracy of 10?m, in the manufactured scaffolds the pore size ranges from 100 to 300?m as that is what is found in trabecular bone. Natural and synthetic polymers were plotted which...

  12. Prediction and Informative Risk Factor Selection of Bone Diseases

    E-Print Network [OSTI]

    Buffalo, State University of New York

    --Electronic health records (EHRs); risk factor analysis; integrated feature extraction; risk factor selection1 Prediction and Informative Risk Factor Selection of Bone Diseases Hui Li, Xiaoyi Li, Murali and the overwhelming amount of electronic health records (EHRs) shared by healthcare institutions and practitioners, we

  13. On the fracture of human dentin: Is it stress-or strain-controlled?

    E-Print Network [OSTI]

    Ritchie, Robert

    On the fracture of human dentin: Is it stress- or strain-controlled? R. K. Nalla,1 J. H. Kinney,2 R information in archival literature that can be usefully used to model such fracture. In fact, although the fracture event in dentin, akin to other mineralized tissues like bone, is widely believed to be locally

  14. Bone quality measurements Osteoporos Int. 2011 Aug;22(8):2225-40. New laboratory tools in the assessment

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    and affect ~30 % of women after the menopause and ~10 % of men. Dual Energy bone densitometry (DXA) has and computer techniques are now available. Table 1: main abbreviations used in the present review DXA Dual Energy bone densitometry BMD bone mineral density BSU bone structure unit microCT microcomputed

  15. The degree of bone mineralization is maintained with single intravenous bisphosphonates in aged estrogen-deficient rats and

    E-Print Network [OSTI]

    Ritchie, Robert

    The degree of bone mineralization is maintained with single intravenous bisphosphonates in aged greater than can be explained by an increase in bone mineral density. In this study, 18-month Fischer 344 to measure lumbar vertebral bone microarchitecture, the degree of bone mineralization (DBM

  16. EVALUATION OF COMPRESSION TESTING METHODS FOR THE CORTICAL RING IN THE DISTAL FEMUR METAPHYSIS OF ADULT MALE RATS 

    E-Print Network [OSTI]

    Bishop, Ryan

    2012-04-26

    . Finkelstein JS, Butler JP, Cleary RL, Neer RM 1994 Compar- ison of four methods for cross-calibrating dual-energy X-ray absorptiometers to eliminate systematic errors when upgrading equipment. J Bone Miner Res 9:1945?1952. 3. Peel NFA, Eastell R 1995...

  17. Development of human brain connectivity in health and disease

    E-Print Network [OSTI]

    Colby, John Benjamin

    2012-01-01

    decreases in cortical thick- ness (discussed later) may beand total z slab thick- ness, subject positioning issues

  18. The Ductility of Human Jaw Bone Attached to a Tooth | Stanford...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    To investigate adaptations of the bony socket, basic principles from tribology, mechanics of materials, and materials science were used to postulate that the ductile nature...

  19. The effect of continuous infusion of human parathyroid hormone on bone architecture in female mice

    E-Print Network [OSTI]

    Eisenberg, Rahel E. (Rahel Esther)

    2009-01-01

    This research sought to create an animal model of secondary hyperparathyroidism through continuous infusion of parathyroid hormone (PTH) in adult female mice, and to subsequently study the catabolic effects of PTH. Osmotic ...

  20. The Ductility of Human Jaw Bone Attached to a Tooth | Stanford Synchrotron

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust,Field-effectWorking With U.S.Week Day Year(active tab) 2016The DarkThe Discovery

  1. Toward Regenerating a Human Thumb In Situ

    E-Print Network [OSTI]

    Weinand, Christian

    Regenerative technology promises to alleviate the problem of limited donor supply for bone or organ transplants.

  2. A spatial property of the retino-cortical mapping ALAN JOHNSTON

    E-Print Network [OSTI]

    Johnston, Alan

    a process of unconscious inference to provide our perception of a three-dimensional world. Though knowledge or physiological model of how this might be achieved. Stevens (1981) has analysed the computational difficulties-trivial. Particular problems arise when we consider this approach as a model for human vision, since in man stimuli

  3. Human Subjects Section 6. Protection of Human

    E-Print Network [OSTI]

    Heller, Barbara

    Human Subjects Section 6. Protection of Human Subjects This section is required for applicants answering "yes" to the question "Are human subjects involved?" on the R&R Other Project Information form subjects applicants must provide a justification in this section for the claim that no human subjects

  4. Enhancement of Growth Performance and Bone Mineralization in Market Broilers through Dietary Enzymes 

    E-Print Network [OSTI]

    Coppedge, Jacob Ryan

    2012-02-14

    phosphorus (aP) in the control diets resulted in improved bird performance and bone ash data. The presence of dietary phytase in phosphorus deficient diets resulted in improvements in growth parameters and bone mineralization. Regression analysis... and processing parameters. In Experiments 3 and 4, a battery trial and a floor trial were conducted to determine the effects of phytase and NSPase enzyme co-administration on growth and bone ash in low phosphorus diets. Increasing the level of available...

  5. Bioactivation of myelotoxic xenobiotics by human neutrophil myeloperoxidase

    SciTech Connect (OSTI)

    Roy, R.R.

    1989-01-01

    Many environmental pollutants and drugs are toxic to the bone marrow. Some of these xenobiotics may initiate toxicity after undergoing bioactivation to free radicals and/or other reactive electrophiles. Peroxidases are a group of enzymes that catalyze the one-electron oxidative bioactivation of a variety of xenobiotics in vitro. Myeloperoxidase (MPO) is a peroxidative enzyme found in very high concentration in the neutrophils of human bone marrow. In this study, human MPO was evaluated to determine its ability to catalyze the in vitro bioactivation of known bone marrow toxicants that contain the aromatic hydroxyl (Ar-OH), aromatic amine (Ar-N-R{sub 2}), or heterocyclic tertiary amine ({double bond}N-R) moieties. The formation of free radical metabolites during the MPO-catalyzed bioactivation of hydroquinone and catechol (benzene metabolites), mitoxantrone and ametantrone (antitumor drugs), and chlorpromazine and promazine (antipsychotic drugs) was demonstrated by EPR spectroscopy. The reactivity of the products formed during the MPO catalyzed bioactivation of ({sup 14}C)hydroquinone and ({sup 14}C)catechol was shown by their covalent binding to protein and DNA in vitro. The covalently binding metabolite in each case is postulated to be the quinone form of the xenobiotic. In addition, both GSH and NADH were oxidized by the reactive intermediate(s) formed during the MPO-catalyzed bioactivation of many of the bone marrow toxicants tested. It was also shown that p,p-biphenol stimulated the MPO catalyzed bioactivation of both hydroquinone and catechol, while p-cresol stimulated the MPO-catalyzed bioactivation of catechol.

  6. NATURE MATERIALS | VOL 14 | JANUARY 2015 | www.nature.com/naturematerials 23 he technological development of humanity was supported

    E-Print Network [OSTI]

    Ritchie, Robert

    NATURE MATERIALS | VOL 14 | JANUARY 2015 | www.nature.com/naturematerials 23 T he technological development of humanity was supported in its early stages by natural materials such as bone, wood and shells. As history advanced, these materials were slowly replaced by synthetic compounds that offered improved perfor

  7. Long-term effects of lead poisoning on bone mineralization in vultures exposed to ammunition sources

    E-Print Network [OSTI]

    Donázar, José A.

    ´n en Recursos Cinege´ticos, IREC (CSIC, UCLM, JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain Bone

  8. Volumetric femoral BMD, bone geometry, and serum sclerostin levels differ between type 2 diabetic postmenopausal women with and without fragility fractures

    E-Print Network [OSTI]

    2015-01-01

    in women and men. J Bone Miner Res 26:373–379 46. Durosierwith diabetes. J Bone Miner Res 27:301–308 8. Patsch JM,fragility fractures. J Bone Miner Res 28: 9. Patsch JM, Li

  9. Dickkopf-1 in Craniofacial Bone and Tooth Development 

    E-Print Network [OSTI]

    Rodgers, Anika Sarah

    2014-08-20

    stream_source_info RODGERS-DISSERTATION-2014.pdf.txt stream_content_type text/plain stream_size 175992 Content-Encoding UTF-8 stream_name RODGERS-DISSERTATION-2014.pdf.txt Content-Type text/plain; charset=UTF-8... DICKKOPF-1 IN CRANIOFACIAL BONE AND TOOTH DEVELOPMENT A Dissertation by ANIKA SARAH RODGERS Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR...

  10. Effects of dietary silicon on bone characteristics of poultry 

    E-Print Network [OSTI]

    Plyler, James Edward

    1977-01-01

    with day-old cockerels, eight-week-old turkey poults, and spent hens, to determine the effects of feeding dietary silicon in the form of sodium meta-silicate on bone character- istics. Growth effects were also determined for the cockerels and poults.... In experiments one and, rt:wo, 832 day-old Single Comb White Leghorn cockerel were fed corn-soy chick starter diets at two calcium levels, 0. 42% and 1. 00%. 'Each level was supplemented with 0, 25, 50, 100, or 200 p. p. m. silicon, in the form of sodium meta...

  11. Zhejiang Bone New Material Technology Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoop IncIowa (UtilityMichigan) JumpZhuyuan ElectricTumuxiHydropower DevelopmentBone New

  12. Patterns of Practice of Palliative Radiotherapy in Africa, Part 1: Bone and Brain Metastases

    SciTech Connect (OSTI)

    Sharma, Vinay Gaye, Papa Macoumba M.Med.; Wahab, Sherif Abdel; Ndlovu, Ntokozo; Ngoma, Twalib; Vanderpuye, Verna; Sowunmi, Anthonia; Kigula-Mugambe, Joseph; Jeremic, Branislav

    2008-03-15

    Purpose: To provide data on the pattern of practice of palliative radiotherapy (RT) on the African continent. Methods and Materials: A questionnaire was distributed to participants in a regional training course of the International Atomic Energy Agency in palliative cancer care and sent by e-mail to other institutions in Africa. Requested information included both infrastructure and human resources available and the pattern of RT practice for metastatic and locally advanced cancers. Results: Of 35 centers contacted, 24 (68%) completed the questionnaire. Although RT is used by most centers for most metastatic cancers, liver and lung metastases are treated with chemotherapy. Of 23 centers, 14 (61%) had a single RT regimen as an institutional policy for treating painful bone metastases, but only 5 centers (23%) of 23 used 8 Gy in 1 fraction. Brain metastases were being treated by RT to the whole brain to 30 Gy in 10 fractions, either exclusively (n = 13, 56%) or in addition to the use of 20 Gy in 5 fractions (n = 3, 14%). Conclusion: Radiotherapy is a major component of treatment of cancer patients in African countries. There is consensus among few centers for treatment schedules for almost all sites regarding time and dose-fractionation characteristics of RT regimens used and/or indications for the use of RT in this setting.

  13. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 675 (2015) | doi:10.1038/bonekey.2015.42

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number declared no competing interests. ORAL POSTERS ­ PRE-CLINICAL The 4th Joint Meeting of ECTS and IBMS

  14. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 685 (2015) | doi:10.1038/bonekey.2015.53

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 POSTMENOPAUSAL OSTEOPOROSIS, DIAGNOSTIC TOOLS

  15. Microfluidic device for bone cell mechanobiology study The use of microfluidics provides many advantages for in vitro biological experiments over typical macro-

    E-Print Network [OSTI]

    Milgram, Paul

    Microfluidic device for bone cell mechanobiology study The use of microfluidics provides many microfluidics to investigate real-time interactions of different bone cells while undergoing physiologically

  16. A Novel Method for the Evaluation of Mechanical Properties of Cancellous Bone in the Rat Distal Femur 

    E-Print Network [OSTI]

    Lucas, Matthew W.

    2010-01-14

    The mechanical properties of the cancellous bone in the laboratory rat animal model are of great interest to the research community for the evaluation of treatments for osteoporosis. Cancellous bone responds rapidly and ...

  17. Development/Plasticity/Repair The Bone Morphogenetic Protein Roof Plate Chemorepellent

    E-Print Network [OSTI]

    Butler, Samantha

    Development/Plasticity/Repair The Bone Morphogenetic Protein Roof Plate Chemorepellent Regulates, Toronto, Ontario, Canada M5G 1X8 Commissural spinal axons extend away from the roof plate (RP) in response to the dorsal midline and are generated by the bone morphogenetic proteins (BMPs) in the roof plate (RP) (Liem

  18. Vitamin K2 Regulation of Bone Homeostasis Is Mediated by the Steroid and Xenobiotic Receptor SXR*

    E-Print Network [OSTI]

    Blumberg, Bruce

    Vitamin K2 Regulation of Bone Homeostasis Is Mediated by the Steroid and Xenobiotic Receptor SXR Institute, The City of Hope National Medical Center, Duarte, California 91010-3000 Vitamin K2 is a critical nutrient required for blood clotting that also plays an important role in bone forma- tion. Vitamin K2

  19. Collaborative Research Opportunities with Henry Ford Health System: Bones, Brains, Blood Pressure, and Beyond

    E-Print Network [OSTI]

    Finley Jr., Russell L.

    , and Beyond Professional and Academic Development (PAD) Seminar Series Tuesday, January 10, 2012 ­ 9:00 to 10Collaborative Research Opportunities with Henry Ford Health System: Bones, Brains, Blood Pressure Research at HFHS HFHS Department of Orthopaedic Surgery's Bone and Joint Center HFHS Department of Internal

  20. Targeting bone-microenvironment-tumour cell interactions : IGF-1 receptor kinase inhibitors. 

    E-Print Network [OSTI]

    Logan, John Gordon

    2012-11-30

    Bone metastases are a frequent clinical complication associated with cancer. The aim of this PhD thesis was to set up a model system for the study of tumour cell – bone cell interactions in vitro, ex vivo and in vivo and ...

  1. Mechanistic aspects of the fracture toughness of elk antler bone M.E. Launey a

    E-Print Network [OSTI]

    Ritchie, Robert

    hydroxyapatite nanoparticles that form a tough, light- weight, adaptive and multi-functional material. Bone-curves a b s t r a c t Bone is an adaptive material that is designed for different functional requirements these functions, it is a dynamic organ that is constantly remodeling and changing shape to adapt to the forces

  2. A Novel 3D Segmentation of Vertebral Bones from Volumetric CT Images Using Graph Cuts

    E-Print Network [OSTI]

    Farag, Aly A.

    of the vertebral bones of spine column with a particular focus on the lumbar spine. The primary goalA Novel 3D Segmentation of Vertebral Bones from Volumetric CT Images Using Graph Cuts Melih S to the Vertebral bodies (VBs). In this paper, we present a novel and fast 3D segmentation framework of VBs

  3. 1174 IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, VOL. 53, NO. 6, JUNE 2006 Microwave Drilling of Bones

    E-Print Network [OSTI]

    Gefen, Amit

    employs lasers for bone drilling [3], however, this method may be too costly for large-scale use1174 IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, VOL. 53, NO. 6, JUNE 2006 Microwave Drilling*, Member, IEEE Abstract--This paper presents a feasibility study of drilling in fresh wet bone tissue

  4. 3D Bone Microarchitecture Modeling and Fracture Risk Department of Computer

    E-Print Network [OSTI]

    Buffalo, State University of New York

    3D Bone Microarchitecture Modeling and Fracture Risk Prediction Hui Li Department of Computer will also rise. It calls for innovative research on understanding of osteoporo- sis and fracture mechanisms-of-the-art probabilistic approach to analyze bone fracture risk factors including demographic attributes and life styles

  5. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 676 (2015) | doi:10.1038/bonekey.2015.43

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number and IBMS Rotterdam, The Netherlands 25­28 April 2015 OP1 (P16) HR-pQCT and DXA Changes in Bone Density

  6. KRFTWRK – Global Human Electricity

    E-Print Network [OSTI]

    Prohaska, Rainer

    2009-01-01

    in the generation of human power, where energy is gained byparticipant runs a virtual human power plant. Per every "by muscle-power and chemical processes of human bodies. For

  7. Bone-cement interface micromechanical model under cyclic loading J.A. Sanz-Herrera1, a

    E-Print Network [OSTI]

    Ariza Moreno, Pilar

    Bone-cement interface micromechanical model under cyclic loading J.A. Sanz-Herrera1, a , H descubrimientos s/n 41092 Seville (Spain) a jsanz@us.es, b helgaem@gmail.com, c mpariza@us.es Keywords: Bone-cement of the last XX century. Normally, implant is fixed to bone by means of a polymer material known as bone cement

  8. Estimation of the 3D self-similarity parameter of trabecular bone from its 2D projection

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    of osteoporosis is mainly based on dual energy X-ray absorptiometry which amounts to measuring bone mass

  9. Human-machine interactions

    DOE Patents [OSTI]

    Forsythe, J. Chris (Sandia Park, NM); Xavier, Patrick G. (Albuquerque, NM); Abbott, Robert G. (Albuquerque, NM); Brannon, Nathan G. (Albuquerque, NM); Bernard, Michael L. (Tijeras, NM); Speed, Ann E. (Albuquerque, NM)

    2009-04-28

    Digital technology utilizing a cognitive model based on human naturalistic decision-making processes, including pattern recognition and episodic memory, can reduce the dependency of human-machine interactions on the abilities of a human user and can enable a machine to more closely emulate human-like responses. Such a cognitive model can enable digital technology to use cognitive capacities fundamental to human-like communication and cooperation to interact with humans.

  10. Jefferson Lab Human Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Resources The Human Resources team is fully integrated with Jefferson Lab's mission, committed to providing quality customer service based on expertise, innovation and...

  11. Reconstitution activity of hypoxic cultured human cord blood CD34-positive cells in NOG mice

    SciTech Connect (OSTI)

    Shima, Haruko; Takubo, Keiyo; Iwasaki, Hiroko; Yoshihara, Hiroki; Gomei, Yumiko; Hosokawa, Kentaro; Arai, Fumio; Takahashi, Takao; Suda, Toshio

    2009-01-16

    Hematopoietic stem cells (HSCs) reside in hypoxic areas of the bone marrow. However, the role of hypoxia in the maintenance of HSCs has not been fully characterized. We performed xenotransplantation of human cord blood cells cultured in hypoxic or normoxic conditions into adult NOD/SCID/IL-2R{gamma}{sup null} (NOG) mice. Hypoxic culture (1% O{sub 2}) for 6 days efficiently supported the maintenance of HSCs, although cell proliferation was suppressed compared to the normoxic culture. In contrast, hypoxia did not affect in vitro colony-forming ability. Upregulation of a cell cycle inhibitor, p21, was observed in hypoxic culture. Immunohistochemical analysis of recipient bone marrow revealed that engrafted CD34{sup +}CD38{sup -} cord blood HSCs were hypoxic. Taken together, these results demonstrate the significance of hypoxia in the maintenance of quiescent human cord blood HSCs.

  12. Increased copy number for methylated maternal 15q duplications leads to changes in gene and protein expression in human cortical samples

    E-Print Network [OSTI]

    Scoles, Haley A; Urraca, Nora; Chadwick, Samuel W; Reiter, Lawrence T; LaSalle, Janine M

    2011-01-01

    melting-curve ana- lysis (MS-HRM) was performed as described50 ng) was then used for MS-HRM on the LightCycler 480 Real-the PWS-IC was per- formed by MS-HRM on the PCR product of

  13. human spaceflight and operations

    E-Print Network [OSTI]

    Anand, Mahesh

    1 human spaceflight and operations Lunar Lander human spaceflight and operations #12;2 human cooperation in exploration prim ary objective opportunity for investigations #12;3 human spaceflight No RHUs LANDING SITE South Polar Reliant on Solar Power generation + conventional thermal control LAUNCHER

  14. Lectin-mediated effects on bone resorption in vitro: a morphological and functional study

    SciTech Connect (OSTI)

    Popoff, S.N.

    1986-01-01

    Lectins have been used to study the structure and function of a variety of cells and tissues. The authors used 4 different lectins, concanavalin A (con A), wheat germ agglutinin (WGA), soybean agglutinin (SBA) and peanut agglutinin (PNA) as in vitro biological probes to study the osteoclast, a multinucleated bone cell that is widely accepted as the primary effector cell responsible for normal bone resorption. They evaluated the effects of each of these lectins on osteoclastic bone resorbing activity and then examined mechanisms that may be responsible for the activation and/or inhibition of osteoclastic activity. Using con A and hemocyanin, a marker molecule used to visualize cell-bound con A via scanning electron microscopy, they demonstrated that osteoclasts have specific con A binding sites on their cell surface. They conducted a series of /sup 45/Ca bone release assays demonstrating that con A has a dose-dependent biphasic effect on bone resorption; stimulation at low concentrations and inhibition at higher concentrations. The findings suggest that the specificity of lectin binding to cell surface receptors may play an important role in the induction of altered cell function. Recently, cells of the mononuclear phagocyte system have been proposed as surrogates of less readily available osteoclasts. They used a macrophage-devitalized bone culture system to evaluate the effects of con A and SBA on the attachment of macrophages to bone and their subsequent functional activity. The results showed that con A, but not SBA, alters the morphology and function of macrophages on a devitalized bone surface. The results support the hypothesis that certain, specific saccharides regulate the interaction between macrophages and bone.

  15. Imaging regenerating bone tissue based on neural networks applied to micro-diffraction measurements

    SciTech Connect (OSTI)

    Campi, G.; Pezzotti, G. [Institute of Crystallography, CNR, via Salaria Km 29.300, I-00015, Monterotondo Roma (Italy)] [Institute of Crystallography, CNR, via Salaria Km 29.300, I-00015, Monterotondo Roma (Italy); Fratini, M. [Centro Fermi -Museo Storico della Fisica e Centro Studi e Ricerche 'Enrico Fermi', Roma (Italy)] [Centro Fermi -Museo Storico della Fisica e Centro Studi e Ricerche 'Enrico Fermi', Roma (Italy); Ricci, A. [Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, D-22607 Hamburg (Germany)] [Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, D-22607 Hamburg (Germany); Burghammer, M. [European Synchrotron Radiation Facility, B. P. 220, F-38043 Grenoble Cedex (France)] [European Synchrotron Radiation Facility, B. P. 220, F-38043 Grenoble Cedex (France); Cancedda, R.; Mastrogiacomo, M. [Istituto Nazionale per la Ricerca sul Cancro, and Dipartimento di Medicina Sperimentale dell'Università di Genova and AUO San Martino Istituto Nazionale per la Ricerca sul Cancro, Largo R. Benzi 10, 16132, Genova (Italy)] [Istituto Nazionale per la Ricerca sul Cancro, and Dipartimento di Medicina Sperimentale dell'Università di Genova and AUO San Martino Istituto Nazionale per la Ricerca sul Cancro, Largo R. Benzi 10, 16132, Genova (Italy); Bukreeva, I.; Cedola, A. [Institute for Chemical and Physical Process, CNR, c/o Physics Dep. at Sapienza University, P-le A. Moro 5, 00185, Roma (Italy)] [Institute for Chemical and Physical Process, CNR, c/o Physics Dep. at Sapienza University, P-le A. Moro 5, 00185, Roma (Italy)

    2013-12-16

    We monitored bone regeneration in a tissue engineering approach. To visualize and understand the structural evolution, the samples have been measured by X-ray micro-diffraction. We find that bone tissue regeneration proceeds through a multi-step mechanism, each step providing a specific diffraction signal. The large amount of data have been classified according to their structure and associated to the process they came from combining Neural Networks algorithms with least square pattern analysis. In this way, we obtain spatial maps of the different components of the tissues visualizing the complex kinetic at the base of the bone regeneration.

  16. IBMS BoneKEy. 2009 April;6(4):132-149 http://www.bonekey-ibms.org/cgi/content/full/ibmske;6/4/132

    E-Print Network [OSTI]

    IBMS BoneKEy. 2009 April;6(4):132-149 http://www.bonekey-ibms.org/cgi/content/full/ibmske;6 and osteoporosis, yet uniquely ­ without targeting the resident fat or bone cell. IBMS BoneKEy. 2009 April;6 with diabetes are afflicted with type 2 disease (5-7). Visceral adiposity and #12;IBMS BoneKEy. 2009 April;6

  17. IBMS BoneKEy. 2010 May;7(5):171-181 http://www.bonekey-ibms.org/cgi/content/full/ibmske;7/5/171

    E-Print Network [OSTI]

    Toledo, University of

    IBMS BoneKEy. 2010 May;7(5):171-181 http://www.bonekey-ibms.org/cgi/content/full/ibmske;7/5/171 doi regulation of energy metabolism. IBMS BoneKEy. 2010 May;7(5):171-181. 2010 International Bone & Mineral energy storage and insulin signaling. The dietary abundance of fatty acids increases the #12;IBMS Bone

  18. Mineral status, bone characterisitics, carcass characteristics, and performance of feedlot lambs implanted with zeranol 

    E-Print Network [OSTI]

    Niemann, Dawn Renee

    1998-01-01

    Fifty-four Rambouillet (avg wt, 34.6 kg) wether lambs were used to determine the effects of zeranol of p and N excretion, animal performance, carcass characteristics, and bone characteristics. Lambs were blocked by sire, ...

  19. Finite-Element Analysis of Biting Behavior and Bone Stress in the

    E-Print Network [OSTI]

    Dumont, Elizabeth R.

    about the biomechanical behavior of bones and muscles under controlled experimental condi- tions possess several types of complex teeth that occupy different functional regions of the mouth. In keeping

  20. The effect of moderate alcohol intake on bone mineral density of ovariectomized rats 

    E-Print Network [OSTI]

    Shipley, Debra Lynn

    1997-01-01

    groups had access to rat chow and distilled water ad libitim. Ovariectomized animals had increased weight and decreased femur density and bone volume per total volume. They also had decreased total trubecular area, trabecular area and number as well...

  1. Non-invasive shock wave stimulated periosteum for bone tissue engineering

    E-Print Network [OSTI]

    Kearney, Cathal (Cathal John)

    2011-01-01

    The cambium cells of the periosteum, which are known osteoprogenitor cells, have limited suitability for clinical applications of bone tissue engineering due to their low cell number (2-5 cells thick). Extracorporeal shock ...

  2. Longitudinal ultrasound measurement of the equine third metacarpal bone as a predictor of mechanical testing properties 

    E-Print Network [OSTI]

    Dyer, Stephanie Ann

    1999-01-01

    diagnostic technique to identify the onset of bucked shins. The purpose of this study was to determine if the longitudinal speed of sound as measured by Soundscan 2000[] was an appropriate predictor of bone strength characterized by mechanical testing...

  3. Methods and modeling for the reduced platen compression of cancellous bone in the rodent proximal tibia 

    E-Print Network [OSTI]

    Rogers, William Elliott

    2002-01-01

    This study focused on the reduced platen compression (RPC) test of cancellous bone in the rodent proximal tibia. The objective was to improve methods for this mechanical test, specifically in the areas of specimen location, specimen preparation...

  4. Relations between the ultrasonic elastic moduli of compact bone and tissue microstructure 

    E-Print Network [OSTI]

    Ahern, John Charles

    1994-01-01

    for Haversian compact bone as homogeneous solid. This study observed that mild changes in the microstructure can predict variations in the mechanical properties. All the tested material properties exhibit gradual increases for small positive changes in ash...

  5. Biochemical markers of bone modeling and remodeling in juvenile racehorses at varying mineral intakes 

    E-Print Network [OSTI]

    Eller, Elena Maria

    2007-09-17

    greater than current NRC recommendations provided a protective effect on the developing skeleton of the young racehorse. Additionally, the biochemical markers used in this study were sensitive enough to track daily changes in bone activity resulting from...

  6. Investigation of bone response to implant materials by electron microscopy and computer simulation

    E-Print Network [OSTI]

    Wang, Hao, 1974-

    2004-01-01

    (cont.) implementation of this scintigraphic method for quantitative studies of osteoblast-mediated mineralization in vitro. A 2-D truss finite element model is used to study the remodeling of trabecular bone. Using strain ...

  7. Analysis of Bone Augmentation With Corticotomy-Facilitated Dental Expansion: A Histologic & Micro-CT Study 

    E-Print Network [OSTI]

    Bare, Britney

    2015-05-08

    The purpose of this study was to evaluate the effect of bone grafting when performed in association with corticotomies. A randomized split-mouth design was utilized in seven adult male beagle dogs. Corticotomies were performed with a piezosurgery...

  8. Self-assembling peptide hydrogels modulate in vitro chondrogenesis of bovine bone marrow stromal cells

    E-Print Network [OSTI]

    Kopesky, Paul Wayne

    Our objective was to test the hypothesis that self-assembling peptide hydrogel scaffolds provide cues that enhance the chondrogenic differentiation of bone marrow stromal cells (BMSCs). BMSCs were encapsulated within two ...

  9. Metabolic modeling for the deposition of transuranic nuclides on bone surfaces 

    E-Print Network [OSTI]

    Halter, Donald Anthony

    1997-01-01

    of plutonium throughout the body has still not been developed. It was determined that the majority of these nuclides are deposited on bone surfaces and are removed with a specific biological half-life, [ ],. ICRP Publication 2 addressed the dosimetry...

  10. Inorganic-Organic Shape Memory Polymers and Foams for Bone Defect Repairs 

    E-Print Network [OSTI]

    Zhang, Dawei

    2013-04-16

    The ultimate goal of this research was to develop a “self-fitting” shape memory polymer (SMP) scaffold for the repair of craniomaxillofacial (CMF) bone defects. CMF defects may be caused by trauma, tumor removal or congenital abnormalities...

  11. Discovery of novel anti-inflammatory proteins inspired by bone marrow mesenchymal stem cell secretions

    E-Print Network [OSTI]

    Milwid, Jack Miles

    2011-01-01

    Bone marrow mesenchymal stem cells (MSCs) may soon become the first FDA-approved stem cell therapy for autoimmune and inflammatory disease. Our lab originally hypothesized that much of the therapeutic activity of MSCs may ...

  12. Mineralization of Decalcified Bone Occurs Under Cell Culture Conditions and Requires Bovine Serum But Not Cells

    E-Print Network [OSTI]

    Price, Paul A.

    on the remineralization of demineralized bone in cell culture medium containing fetal bovine serum. Demineralized newborn to demineralization. Materials and Methods Materials Forty-day-old male rats (Sprague­Dawley-derived) were pur- chased

  13. Classification and volumetric analysis of temporal bone pneumatization using cone beam computed tomography

    E-Print Network [OSTI]

    Terasaki, Mark

    Classification and volumetric analysis of temporal bone pneumatization using cone beam computed Objective. This study performed volumetric analysis and classified different repeated patterns of temporal. Volumetric analysis of the pneumatization was performed using a window thresholding procedure on multiplanar

  14. Factors Affecting the Mechanical Behavior of Bone Subrata Saha, Ph.D.

    E-Print Network [OSTI]

    Gilbert, Robert P.

    Factors Affecting the Mechanical Behavior of Bone by Subrata Saha, Ph.D. Research Professor-mail: subrata.saha@downstate.edu ABSTRACT The load carrying capacity of our skeletal system depends

  15. Calcium balance and bone density in immature horses fed a high protein diet 

    E-Print Network [OSTI]

    Spooner, Holly Sue

    2006-10-30

    Studies in other species indicate high protein diets increase urinary calcium (Ca) excretion and may lead to negative calcium balance and reduced bone density. As overfeeding of protein is commonplace in the horse ...

  16. Bone Marrow Stem Cell Derived Paracrine Factors for Regenerative Medicine: Current Perspectives and Therapeutic Potential

    E-Print Network [OSTI]

    Burdon, Tom J.; Paul, Arghya; Noiseux, Nicolas; Prakash, Satya; Shum-Tim, Dominique

    2011-01-01

    During the past several years, there has been intense research in the field of bone marrow-derived stem cell (BMSC) therapy to facilitate its translation into clinical setting. Although a lot has been accomplished, plenty of challenges lie ahead...

  17. Programming with human computation

    E-Print Network [OSTI]

    Little, Greg (Danny Greg)

    2011-01-01

    Amazon's Mechanical Turk provides a programmatically accessible micro-task market, allowing a program to hire human workers. This has opened the door to a rich field of research in human computation where programs orchestrate ...

  18. Patenting Human Evolution

    E-Print Network [OSTI]

    Torrance, Andrew W.

    2008-06-01

    to thorough analysis and debate prior to the imminent arrival of human genetic enhancement technologies. Otherwise, patent law may drive human evolution in directions either unplanned - or worse - undesired....

  19. EFFECT OF DIETARY GLYCOMACROPEPTIDE AND CHOLESTEROL ON CORTICAL GANGLIOSIDE- AND GLYCOPROTEIN-BOUND N-ACETYLNEURAMINIC ACID IN YOUNG RATS

    E-Print Network [OSTI]

    Kary, Susan Ann

    2009-04-29

    Background: Sialic acid and cholesterol are present in human milk and accumulate rapidly in the brain during development. Infant formulas contain little sialic acid or cholesterol. Sialic acid and cholesterol supplementation ...

  20. Human Functional Brain Imaging

    E-Print Network [OSTI]

    Rambaut, Andrew

    Human Functional Brain Imaging 1990­2009 September 2011 Portfolio Review #12;2 | Portfolio Review: Human Functional Brain ImagingThe Wellcome Trust is a charity registered in England and Wales, no's role in supporting human functional brain imaging and have informed `our' speculations for the future

  1. Developments in Human Communication

    E-Print Network [OSTI]

    Thompson, Michael

    CMST 1B03 Developments in Human Communication: Part & Present Amanda Etches-Johnson Mills Research in Human Communication Session Outline 1. Finding Books Using MORRIS 2. Finding Journal Articles using: Developments in Human Communication What is MORRIS anyway? · MORRIS is McMaster's library catalogue · Shows

  2. Sandia Energy - Human Reliability Assessment

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Reliability Assessment Home Stationary Power Nuclear Fuel Cycle Nuclear Energy Safety Technologies Risk and Safety Assessment Human Reliability Assessment Human Reliability...

  3. Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Grandfield, Kathryn; Herber, Ralf -Peter; Chen, Ling; Djomehri, Sabra; Tam, Caleb; Lee, Ji -Hyun; Brown, Evan; Woolwine III, Wood R.; Curtis, Don; Ryder, Mark; et al

    2015-04-18

    Objective: The objective of this study was to investigate the effect of mechanical strain by mapping physicochemical properties at periodontal ligament (PDL)–bone and PDL–cementum attachment sites and within the tissues per se. Design: Accentuated mechanical strain was induced by applying a unidirectional force of 0.06 N for 14 days on molars in a rat model. The associated changes in functional space between the tooth and bone, mineral forming and resorbing events at the PDL–bone and PDL–cementum attachment sites were identified by using micro-X-ray computed tomography (micro-XCT), atomic force microscopy (AFM), dynamic histomorphometry, Raman microspectroscopy, and AFM-based nanoindentation technique. Results frommore »these analytical techniques were correlated with histochemical strains specific to low and high molecular weight GAGs, including biglycan, and osteoclast distribution through tartrate resistant acid phosphatase (TRAP) staining. Results: Unique chemical and mechanical qualities including heterogeneous bony fingers with hygroscopic Sharpey's fibers contributing to a higher organic (amide III — 1240 cm?¹) to inorganic (phosphate — 960 cm?¹) ratio, with lower average elastic modulus of 8 GPa versus 12 GPa in unadapted regions were identified. Furthermore, an increased presence of elemental Zn in cement lines and mineralizing fronts of PDL–bone was observed. Adapted regions containing bony fingers exhibited woven bone-like architecture and these regions rich in biglycan (BGN) and bone sialoprotein (BSP) also contained high-molecular weight polysaccharides predominantly at the site of polarized bone growth. Conclusions: From a fundamental science perspective the shift in local properties due to strain amplification at the soft–hard tissue attachment sites is governed by semiautonomous cellular events at the PDL–bone and PDL–cementum sites. Over time, these strain-mediated events can alter the physicochemical properties of tissues per se, and consequently the overall biomechanics of the bone–PDL–tooth complex. From a clinical perspective, the shifts in magnitude and duration of forces on the periodontal ligament can prompt a shift in physiologic mineral apposition in cementum and alveolar bone albeit of an adapted quality owing to the rapid mechanical translation of the tooth.« less

  4. Human Factors @ UB Fall 2010 Human Factors

    E-Print Network [OSTI]

    Krovi, Venkat

    . Outsourcing aviation maintenance: Hu- man factors implications, specifically for communications. C. Drury, K. Guy, C. Wenner. International Journal of Aviation Psychology, 2010, 20, 124 ­ 143. #12;2 Human Factors

  5. Salient features in locomotor evolutionary adaptations of proboscideans revealed via the differential scaling of limb long bones

    E-Print Network [OSTI]

    Kokshenev, Valery B

    2009-01-01

    The standard differential scaling of proportions in limb long bones (length against circumference) is applied to a phylogenetically wide sample of the Proboscidea, Elephantidae and the Asian (Elephas maximus) and African elephant (Loxodonta africana). In order to investigate allometric patterns in proboscideans and terrestrial mammals with parasagittal limb kinematics, the computed slopes (slenderness exponents) are compared with published values for mammals and studied within a framework of theoretical models of long bone scaling under gravity and muscle forces. Limb bone allometry in E. maximus and the Elephantidae are congruent with adaptation to bending and/or torsion induced by muscular forces during fast locomotion, as in other mammals, whereas limb bones in L. africana appear adapted for coping with the compressive forces of gravity. Consequently, hindlimb bones are expected to be more compliant than forelimb bones in accordance with in vivo studies on elephant locomotory kinetics and kinematics, and t...

  6. Effect of 710 nm visible light irradiation on neurite outgrowth in primary rat cortical neurons following ischemic insult

    SciTech Connect (OSTI)

    Choi, Dong-Hee [Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University, Seoul (Korea, Republic of) [Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University, Seoul (Korea, Republic of); Department of Medical Science, Konkuk University School of Medicine, Seoul (Korea, Republic of); Lee, Kyoung-Hee; Kim, Ji-Hye; Kim, Moon Young [Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University, Seoul (Korea, Republic of)] [Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University, Seoul (Korea, Republic of); Lim, Jeong Hoon [Department of Rehabilitation Medicine, Konkuk University School of Medicine, Seoul (Korea, Republic of) [Department of Rehabilitation Medicine, Konkuk University School of Medicine, Seoul (Korea, Republic of); Rehabilitation Medicine, Division of Neurology, Department of Medicine, National University Hospital, National University Health System (Singapore); Lee, Jongmin, E-mail: leej@kuh.ac.kr [Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University, Seoul (Korea, Republic of) [Center for Neuroscience Research, SMART Institute of Advanced Biomedical Science, Konkuk University, Seoul (Korea, Republic of); Department of Rehabilitation Medicine, Konkuk University School of Medicine, Seoul (Korea, Republic of)

    2012-06-01

    Highlights: Black-Right-Pointing-Pointer 710 nm wavelength light (LED) has a protective effect in the stroke animal model. Black-Right-Pointing-Pointer We determined the effects of LED irradiation in vitro stroke model. Black-Right-Pointing-Pointer LED treatment promotes the neurite outgrowth through MAPK activation. Black-Right-Pointing-Pointer The level of synaptic markers significantly increased with LED treatment. Black-Right-Pointing-Pointer LED treatment protects cell death in the in vitro stroke model. -- Abstract: Objective: We previously reported that 710 nm Light-emitting Diode (LED) has a protective effect through cellular immunity activation in the stroke animal model. However, whether LED directly protects neurons suffering from neurodegeneration was entirely unknown. Therefore, we sought to determine the effects of 710 nm visible light irradiation on neuronal protection and neuronal outgrowth in an in vitro stroke model. Materials and methods: Primary cultured rat cortical neurons were exposed to oxygen-glucose deprivation (OGD) and reoxygenation and normal conditions. An LED array with a peak wavelength of 710 nm was placed beneath the covered culture dishes with the room light turned off and were irradiated accordingly. LED treatments (4 min at 4 J/cm{sup 2} and 50 mW/cm{sup 2}) were given once to four times within 8 h at 2 h intervals for 7 days. Mean neurite density, mean neurite diameter, and total fiber length were also measured after microtubule associated protein 2 (MAP2) immunostaining using the Axio Vision program. Synaptic marker expression and MAPK activation were confirmed by Western blotting. Results: Images captured after MAP2 immunocytochemistry showed significant (p < 0.05) enhancement of post-ischemic neurite outgrowth with LED treatment once and twice a day. MAPK activation was enhanced by LED treatment in both OGD-exposed and normal cells. The levels of synaptic markers such as PSD 95, GAP 43, and synaptophysin significantly increased with LED treatment in both OGD-exposed and normal cells (p < 0.05). Conclusion: Our data suggest that LED treatment may promote synaptogenesis through MAPK activation and subsequently protect cell death in the in vitro stroke model.

  7. Electron Microscopy and Analytical X-ray Characterization of Compositional and Nanoscale Structural Changes in Fossil Bone

    E-Print Network [OSTI]

    Boatman, Elizabeth

    2012-01-01

    of questions surrounding the diagenesis and fossilization ofthe consequences of diagenesis for that particular feature (on the concept of bone diagenesis and how it relates to

  8. A Bone Density Based Finite Element Study of the Efficacy of Maxillary Protraction Protocols With and Without Mini-Implants

    E-Print Network [OSTI]

    Chu, Howard

    2015-01-01

    accurate location and material properties of the bone andthe dissimilar material properties and capturing the localfinite elements. The material properties of the craniofacial

  9. Synchrotron imaging reveals bone healing and remodelling strategies in extinct and extant vertebrates

    SciTech Connect (OSTI)

    Anne, Jennifer [Univ. of Manchester (United Kingdom); Edwards, Nicholas P. [Univ. of Manchester (United Kingdom); Wogelius, Roy A. [Univ. of Manchester (United Kingdom); Tumarkin-Deratzian, Allison R. [Temple Univ., Philadelphia, PA (United States); Sellers, William I. [Univ. of Manchester (United Kingdom); van Veelen, Arjen [Univ. of Manchester (United Kingdom); Bergmann, Uwe [SLAC National Accelerator Laboratory, Menlo Park, CA (United States); Sokaras, Dimosthenis [SLAC National Accelerator Laboratory, Menlo Park, CA (United States); Alonso-Mori, Roberto [SLAC National Accelerator Laboratory, Menlo Park, CA (United States); Ignatyev, Konstantin [Diamond Light Source (United Kingdom); Egerton, Victoria M. [Univ. of Manchester (United Kingdom); Manning, Phillip L. [Univ. of Manchester (United Kingdom)

    2014-04-23

    Current understanding of bone healing and remodelling strategies in vertebrates has traditionally relied on morphological observations through the histological analysis of thin sections. However, chemical analysis may also be used in such interpretations, as different elements are known to be absorbed and used by bone for different physiological purposes such as growth and healing. These chemical signatures are beyond the detection limit of most laboratory-based analytical techniques (e.g. scanning electron microscopy). However, synchrotron rapid scanning–X-ray fluorescence (SRS–XRF) is an elemental mapping technique that uniquely combines high sensitivity (ppm), excellent sample resolution (20–100 ?m) and the ability to scan large specimens (decimetre scale) approximately 3000 times faster than other mapping techniques. Here, we use SRS–XRF combined with microfocus elemental mapping (2–20 ?m) to determine the distribution and concentration of trace elements within pathological and normal bone of both extant and extinct archosaurs (Cathartes aura and Allosaurus fragilis). Results reveal discrete chemical inventories within different bone tissue types and preservation modes. Chemical inventories also revealed detail of histological features not observable in thin section, including fine structures within the interface between pathological and normal bone as well as woven texture within pathological tissue.

  10. Synchrotron imaging reveals bone healing and remodelling strategies in extinct and extant vertebrates

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Anne, Jennifer; Edwards, Nicholas P.; Wogelius, Roy A.; Tumarkin-Deratzian, Allison R.; Sellers, William I.; van Veelen, Arjen; Bergmann, Uwe; Sokaras, Dimosthenis; Alonso-Mori, Roberto; Ignatyev, Konstantin; et al

    2014-04-23

    Current understanding of bone healing and remodelling strategies in vertebrates has traditionally relied on morphological observations through the histological analysis of thin sections. However, chemical analysis may also be used in such interpretations, as different elements are known to be absorbed and used by bone for different physiological purposes such as growth and healing. These chemical signatures are beyond the detection limit of most laboratory-based analytical techniques (e.g. scanning electron microscopy). However, synchrotron rapid scanning–X-ray fluorescence (SRS–XRF) is an elemental mapping technique that uniquely combines high sensitivity (ppm), excellent sample resolution (20–100 ?m) and the ability to scan large specimensmore »(decimetre scale) approximately 3000 times faster than other mapping techniques. Here, we use SRS–XRF combined with microfocus elemental mapping (2–20 ?m) to determine the distribution and concentration of trace elements within pathological and normal bone of both extant and extinct archosaurs (Cathartes aura and Allosaurus fragilis). Results reveal discrete chemical inventories within different bone tissue types and preservation modes. Chemical inventories also revealed detail of histological features not observable in thin section, including fine structures within the interface between pathological and normal bone as well as woven texture within pathological tissue.« less

  11. Jefferson Lab Human Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    JLab Diversity Policies 200 Human Resources 202 Equal Employment Opportunity and Affirmative Action 203 Employment 208 Employee Performance and Conduct 209 Staff Development 210...

  12. ORISE: Human Subjects Protection

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human Subjects Protection The Oak Ridge Institute for Science and Education (ORISE) performs technical assessments to assist U.S. Department of Energy (DOE) laboratories involved...

  13. Mapping Callosal Morphology in Early-and Late-Onset Elderly Depression: An Index of Distinct Changes in Cortical

    E-Print Network [OSTI]

    Thompson, Paul

    MRI data, novel mesh-based geometrical modeling methods were applied to compare the midsagittal of California at Los Angeles, Los Angeles, CA, USA; 3 Department of Biomedical Sciences & Biotechnologies Institute for Neuroscience and Human Behavior, University of California at Los Angeles, Los Angeles, CA, USA

  14. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 680 (2015) | doi:10.1038/bonekey.2015.47

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number PARALLEL PROGRAMME The 4th Joint Meeting of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 CABS

  15. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 677 (2015) | doi:10.1038/bonekey.2015.44

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number Joint Meeting of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 OC1.1 Wnt16 Promotes

  16. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 684 (2015) | doi:10.1038/bonekey.2015.52

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number Meeting of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 CECR 1 Which Came First: Parathyroid

  17. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 674 (2015) | doi:10.1038/bonekey.2015.41

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number POSTERS - CABS The 4th Joint Meeting of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 CABS OP1

  18. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS Citation: IBMS BoneKEy 3, Article number: 673 (2015) | doi:10.1038/bonekey.2015.40

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS Citation: IBMS BoneKEy 3, Article number.86 [95% CI=0.78-0.94]) and THR POSTER PRESENTATIONS The 4th Joint Meeting of ECTS and IBMS Rotterdam

  19. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 682 (2015) | doi:10.1038/bonekey.2015.50

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 D1.1 Abstract not available D1.2 This House

  20. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 681 (2015) | doi:10.1038/bonekey.2015.49

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number reserved 1940-8692/15 www.nature.com/bonekey WORKSHOP ABSTRACTS The 4th Joint Meeting of ECTS and IBMS

  1. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 683 (2015) | doi:10.1038/bonekey.2015.51

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number ABSTRACTS The 4th Joint Meeting of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 S1.1 Abstract

  2. IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article number: 678 (2015) | doi:10.1038/bonekey.2015.45

    E-Print Network [OSTI]

    Cai, Long

    2015-01-01

    IBMS BoneKEy | The 4th Joint Meeting of ECTS and IBMS 1 Citation: IBMS BoneKEy 13, Article numberth Joint Meeting of ECTS and IBMS Rotterdam, The Netherlands 25­28 April 2015 HT1 High Serum Levels

  3. Phenotypic characterization of early events of thymus repopulation in radiation bone marrow chimeras

    SciTech Connect (OSTI)

    Sharrow, S.O.; Singer, A.; Hammerling, U.; Mathieson, B.J.

    1983-04-01

    The phenotype of murine thymocytes repopulating the thymus of radiation bone marrow chimeras shortly after irradiation and bone marrow reconstitution was analyzed by immunofluorescence and flow microfluorometry. Thymuses in these chimeras, while essentially devoid of lymphoid cells at day 7, were repopulated by days 10 to 12 after irradiation. It was found that this initial repopulation arose from a radioresistant intrathymic precursor that expanded to an almost complete complement of host-type thymocytes. However, these host-derived thymocytes were unusual in that they were relatively deficient in Lyt 1+2- and peanut agglutinin ''dull'' cells as compared with normal thymocytes. Donor bone-marrow-derived cells first appeared in the irradiated chimeric thymuses between days 12 and 15 after irradiation and bone marrow transfer. By day 19, chimeric thymuses contained more than 98% donor cells. This course was identical for three chimeric combinations, each made across different genetic barriers. In contrast to the cells that populate the fetal thymus during normal ontogeny, the first donor bone-marrow-derived cells that can be detected within the irradiated chimeric thymuses already expressed phenotypically normal adult T cell subpopulations in that they contained significant numbers both of Lyt 1+2- and of Lyt 1+2+ thymocytes. Thus, the Lyt phenotype of donor cells that initially repopulate an adult thymus after irradiation is markedly different from the Lyt phenotype of cells that initially populate the fetal thymus. The differences between adult and fetal thymic development that are observed in radiation bone marrow chimeras may be important in our understanding of T cell differentiation in these animals.

  4. A novel patient-derived intra-femoral xenograft model of bone metastatic prostate cancer that recapitulates mixed osteolytic and osteoblastic lesions.

    E-Print Network [OSTI]

    2011-01-01

    cancer [4,17-19]. Direct injection of prostate can- cer4,20,21]. However, direct injection models into the bone-24]. Page 2 of 13 Direct bone-injection murine xenograft

  5. Development of a three-dimensional in vitro model to study the effect of vitamin D on bone metastatic breast cancer

    E-Print Network [OSTI]

    Li, Danda

    2011-08-31

    Breast cancer has a high prevalence among women and most patients suffer from metastasis to bone. The mechanisms involved in breast cancer bone metastasis are poorly understood. Three-dimensional (3D) tissue culture systems are becoming a focus...

  6. Hierarchical interconnections in the nano-composite material bone: Fibrillar cross-links resist fracture on several length scales

    E-Print Network [OSTI]

    Lipman, Everett A.

    to its biomedical significance, bone has been used as a model for many artificial bio-ceramic composites [10,11]. In many of these artificial composites, a combina- tion of a soft polymer matrix reinforcedHierarchical interconnections in the nano-composite material bone: Fibrillar cross-links resist

  7. SYMPOSIUM: SEX DIFFERENCES IN MUSCULOSKELETAL DISEASE AND SCIENCE b-Ecdysone Augments Peak Bone Mass in Mice of Both Sexes

    E-Print Network [OSTI]

    Ritchie, Robert

    SYMPOSIUM: SEX DIFFERENCES IN MUSCULOSKELETAL DISEASE AND SCIENCE b-Ecdysone Augments Peak Bone Mass in Mice of Both Sexes Weiwei Dai PhD, HongLiang Zhang MD, PhD, Zhendong A. Zhong PhD, Li Jiang MD to investigate sex differences in terms of bone development and structure after bEcd administration. Methods Two

  8. Prevention of Postmenopausal Bone Loss by a Low-Magnitude, High-Frequency Mechanical Stimuli: A Clinical Trial Assessing Compliance,

    E-Print Network [OSTI]

    words: osteoporosis, anabolic, mechanical loading, vibration, low-level, frequency, osteogenic, musclePrevention of Postmenopausal Bone Loss by a Low-Magnitude, High- Frequency Mechanical Stimuli) of a low-level (0.2g, 30 Hz) vibration applied during quiet standing can effectively inhibit bone loss

  9. A Serum Factor that Recalcifies Demineralized Bone Is Conserved in Bony Fish and Sharks but Is Not Found in Invertebrates

    E-Print Network [OSTI]

    Price, Paul A.

    A Serum Factor that Recalcifies Demineralized Bone Is Conserved in Bony Fish and Sharks fish -- Cartilagi- nous fish We recently showed that the type I collagen matrix of demineralized bone and phosphate into demineralized rat tibias ceases after several weeks of incubation in suc- cessive volumes

  10. COMBINING CLASSIFIERS FOR BONE FRACTURE DETECTION IN X-RAY IMAGES Vineta Lai Fun Lum, Wee Kheng Leow, Ying Chen,

    E-Print Network [OSTI]

    Leow, Wee Kheng

    COMBINING CLASSIFIERS FOR BONE FRACTURE DETECTION IN X-RAY IMAGES Vineta Lai Fun Lum, Wee Kheng combination methods ap- plied to the detection of bone fractures in x-ray images. Test results show, only about 12% of them contained fractured femurs. For radius images, about 30% of 145 consecu- tive

  11. The human genome project

    SciTech Connect (OSTI)

    Yager, T.D.; Zewert, T.E.; Hood, L.E. )

    1994-04-01

    The Human Genome Project (HGP) is a coordinated worldwide effort to precisely map the human genome and the genomes of selected model organisms. The first explicit proposal for this project dates from 1985 although its foundations (both conceptual and technological) can be traced back many years in genetics, molecular biology, and biotechnology. The HGP has matured rapidly and is producing results of great significance.

  12. HUMANITIES & SOCIAL SCIENCES

    E-Print Network [OSTI]

    Yang, Eui-Hyeok

    , The Role of Technology in Film, and even the History of Aeronautics-- in each case, at the request of the students. HUMANITIES & SOCIAL SCIENCES STEVENS INSTITUTE OF TECHNOLOGY www.stevens.edu MAJORS: + History in this ever- changing, fast-paced, technology-driven world. They also investigate how human in- novation

  13. Human Functional Brain Imaging

    E-Print Network [OSTI]

    Rambaut, Andrew

    Human Functional Brain Imaging 1990­2009 September 2011 Portfolio Review Summary Brain Imaging #12 Dale ­ one of our first Trustees. Understanding the brain remains one of our key strategic aims today three-fold: · to identify the key landmarks and influences on the human functional brain imaging

  14. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-12-20

    The order establishes Department of Energy (DOE) procedures and responsibilities for implementing the policy and requirements set forth in 10 Code of Federal Regulations (CFR) Part 745, Protection of Human Subjects; and in DOE P 443.1A, Protection of Human Subjects, dated 12-20-07. Cancels DOE O 443.1. Canceled by DOE O 443.1B.

  15. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2000-05-15

    To establish DOE procedures and responsibilities for implementing the policy and requirements set forth in 10 CFR Part 745, Protection of Human Subjects, ad in DOE P 443.1, Policy on the Protection of Human Subjects. Cancels DOE O 1300.3. Canceled by DOE O 443.1A.

  16. Methods for identifying cancellous bone specimen location and size for the Reduced Platen Compression Test 

    E-Print Network [OSTI]

    Cowen, Kyle Ray

    2013-02-22

    the locatron of the RPt: 'lest specimen within the femur. In previous tests, SigmaScan/Image was used to measure thc distance from the distal end of the femur to the drstal extent of the epiphyseal growth plate. The distance was averaged for the entire tesi... Measurement of Bone: A Tutorial. Bone. 14, 595-608, VITA Ky!e Ray Cowen was born tn Amarillo, Texas on February 17, 197'7 to Alvin and Carol Cowen. Kyle is the youngest of two children, the oldest bc!ng his sister Kans who was burr. c:! Decemder?, 1973...

  17. Feeding Bone Meal to Range Cattle on the Coastal Plains of Texas : Preliminary Report. 

    E-Print Network [OSTI]

    Schmidt, H.

    1926-01-01

    .. Chref I FAR L. B. G. v. * *T. **J. M AND RANCH ECONOMICS: P GABBARD b1 S Chief YOUNGBLOO~, M.'s., Ph. D., Farm d . Ranch Economrst. , L. CRAWFORD, M.-S., Research Mark& Specralrst L. CORY, M. S.. Grazing Research Botanil L. GASTON, JR., B...~on with U. S. Department.of Agriculture ***In cooperation with the School of Agriculture. SYNOPSIS X is Bulletin reports results obtained by feeding bone meal and salt mixtures and finely ground rock phosphate mixed with bone meal and salt to cattle...

  18. Department of Humanities Program in Technology and the Humanities

    E-Print Network [OSTI]

    ; ethics in the professions; history of art and architecture; humanizing technology; information seek- ingHumanities Department of Humanities Program in Technology and the Humanities Siegel Hall 218 3301 S' Graduate Program in Technology and Humanities prepares students for careers in emerging forms of technology

  19. Multivariate Statistics of Tensor-Based Cortical Surface Morphometry Y. Wang1,2, X. Gu3, T. F Chan2, A. W Toga1, P. M Thompson1

    E-Print Network [OSTI]

    Thompson, Paul

    Multivariate Statistics of Tensor-Based Cortical Surface Morphometry Y. Wang1,2, X. Gu3, T. F Chan2, United States Introduction: We propose a new set of multivariate statistics of tensor-based morphometric and 40 are from William Syndrome. We applied the multivariate statistics of tensor based morphometry

  20. Bone Loss in Diabetes: Use of Antidiabetic Thiazolidinediones and Secondary Osteoporosis

    E-Print Network [OSTI]

    Toledo, University of

    used TZDs (pioglitazone and rosiglita- zone) by using combinational therapy with approved antiosteoporotic drugs, or by using lower doses of TZDs in combination with other antidiabetic therapy. We also healing in T2DM patients on TZD therapy. Keywords Diabetes . Thiazolidinediones . Bone . Osteoporosis

  1. Kidney, liver and bone cadmium content in the western sandpiper in relation to migration

    E-Print Network [OSTI]

    Kidney, liver and bone cadmium content in the western sandpiper in relation to migration C. N. Mc the Pacific Coast, at stopover sites as the birds migrate north to Alaska and south to Panama. This study ingestion of metal bearing sediments.1 These sediment-dwelling organisms in turn are primary food sources

  2. Stability and Three-Dimensional Analysis of Bone Formation in Longitudinally Fluted Miniscrew Implants 

    E-Print Network [OSTI]

    Truong, An Van

    2014-04-22

    The purpose of the present study is to evaluate the effects of longitudinal flutes on mini-screw implant (MSI) bone healing and stability. Using 11 skeletally mature New Zealand White rabbits, 33 longitudinally fluted and 33 non-fluted MSIs were...

  3. Patient-Specific FE Analyses of Metatarsal Bones with1 Inhomogeneous Isotropic Material Properties2

    E-Print Network [OSTI]

    Yosibash, Zohar

    research and clinical practice, especially when associated with the9 correction of Hallux Valgus. Verified to Hallux14 Valgus correction are then drilled in the bones, which are then reloaded until15 fracture on the mechanical response so to optimize the outcome of the Hallux Valgus27 correction.28 This study further

  4. Microfluidic purification and analysis of hematopoietic stem cells from bone Romana Schirhagl,a

    E-Print Network [OSTI]

    Zare, Richard N.

    Microfluidic purification and analysis of hematopoietic stem cells from bone marrow Romana to separate them from a whole-marrow sample. A microfluidic device was fabricated using an integrated membrane are restricted by the limited availability of stem cell sources.2,3 We believe that microfluidics can be used

  5. JOURNAL OF BONE AND MINERAL RESEARCH Volume 7, Number 12, 1992

    E-Print Network [OSTI]

    Chuong, Cheng-Ming

    JOURNAL OF BONE AND MINERAL RESEARCH Volume 7, Number 12, 1992 Mary Ann Liebert, Inc., Publishers behavior and molecular ex- pression before it finally becomes a mineralizing osteocyte. The progression, extracellular maturation, and mineralization -are identified. (3.4) Many molecules functioning as growth fac

  6. Elemental concentrations in bones from an ancient Egyptian mummy and from a recent man

    SciTech Connect (OSTI)

    Cholewa, M.; Kwiatek, W.M.; Jones, K.W.; Schidlovsky, G.; Paschoa, A.S.; Miller, S.C.; Pecotte, J.

    1986-06-01

    Differences in elemental concentrations in bones taken from an ancient Egyptian mummy and a contemporary man were investigated by using proton induced x-ray emission (PIXE) in combination with Rutherford backscattering (RBS). Remarkable differences were noticed in the Fe/Ca and Pb/Ca relative concentrations, which were consistently higher in the contemporary man. 5 refs., 2 figs., 2 tabs.

  7. School of Architecture, Design and the Built Environment Project Title: Artificial bone for prosthetic hip joints

    E-Print Network [OSTI]

    Evans, Paul

    formation by the Additive Manufacturing (AM) direct printing process. The artificial bone must and the development of new additive manufacturing techniques for medical devices. The group has active links and structural gradients into the prosthesis. It is envisioned this could involve the use of additively

  8. The basic tenet of skeletochronology is that bone growth is cyclic and has an

    E-Print Network [OSTI]

    United States. and amphibians include double (Chin- samy et al., 1995; El Mouden et al., 1997; Guarino et's ridley sea turtles from a comparison of death date and amount of bone growth following the comple- tion-at-age and growth rates can be estimated from dimen- sions of early growth marks. These results validate

  9. Corrigendum Mechanics of limb bone loading during terrestrial locomotion in river cooter turtles

    E-Print Network [OSTI]

    Blob, Richard W.

    bending and shear stress and of safety factors in bending were unaffected by this error, but the mean and conclusions of the original paper remain unchanged. REFERENCES Biewener, A. A. and Dial, K. P. (1995). In vivo. concinna Bending Torsion Bone N Yield stress (MPa) Safety factor mean N Yield stress (MPa) Safety factor

  10. Mitigating Disuse Bone Loss: Role of Resistance Exercise and Beta-Adrenergic Signaling 

    E-Print Network [OSTI]

    Swift, Joshua Michael

    2011-08-08

    . Recent data gathered from crew members on the International Space Station (ISS) illustrates the significant losses of bone mineral density (BMD) and geometry of the femoral neck (15). Dual-energy x-ray absorptiometry (DXA) and QCT scans were taken...

  11. A 3D Statistical Shape Model Of The Pelvic Bone For Segmentation

    E-Print Network [OSTI]

    Andrzejak, Artur

    patient models from 3D image data. Within the setting of a hybrid system (applicator plus MR tomograph. Left: hybrid system (MRT plus applicator), Right: MRT slice image from the abdomen with pelvic bone. 1 on heating up affected tissue compartments to temperatures above 42 degree Celsius without damaging

  12. Trabecular Bone Structure and Cellular Morphology in the Primate Craniofacial Skeleton 

    E-Print Network [OSTI]

    Pryor, Leslie Claire

    2015-08-14

    BONE STRUCTURE AND CELLULAR MORPHOLOGY IN THE PRIMATE CRANIOFACIAL SKELETON A Dissertation by LESLIE CLAIRE PRYOR Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment... Emet Schneiderman David Strait Head of Department, Paul C. Dechow August 2015 Major Subject: Biomedical Sciences Copyright 2015 Leslie Claire Pryor ii ABSTRACT The primate craniofacial skeleton is complex...

  13. On Smoothing Surfaces in Voxel Based Finite Element Analysis of Trabecular Bone

    E-Print Network [OSTI]

    Frey, Pascal

    On Smoothing Surfaces in Voxel Based Finite Element Analysis of Trabecular Bone Peter Arbenz on complicated domains composed of often hundreds of millions of voxel elements. The finite element analysis finite element (FE) analysis. The approach based on the FE analysis leads to linear systems of equations

  14. Mechanical regulation of signaling pathways in bone, William R. Thompson a,

    E-Print Network [OSTI]

    , cyclooxygenase 2; LMMS, low magnitude mechanical stimulation; LIV, low intensity vibration; CT, micro compReview Mechanical regulation of signaling pathways in bone, William R. Thompson a, , Clinton T A wide range of cell types depend on mechanically induced signals to enable appropriate physiological re

  15. The consequence of late-onset alcohol abuse in aged bone: a histomorhometric analysis 

    E-Print Network [OSTI]

    Barker, Lisa Setchfield

    2000-01-01

    The objective of this experiment was to examine the effect of late-onset alcohol abuse on aged bone using the rat model. Thirty female Fischer 344 rats were separated by weights into one of four groups: baseline, alcohol-fed, pair-fed, and pellet...

  16. Parahippocampal and retrosplenial contributions to human spatial

    E-Print Network [OSTI]

    Epstein, Russell A.

    navigational tasks and also during passive viewing of navigationally relevant stimuli such as environmental parahippocampal and retro- splenial cortices [4­10], regions that also respond strongly during passive viewing' or `house' area [13,14,22], its response to buildings is smaller than the response to scenes [11

  17. Special Issue on Human Computing

    E-Print Network [OSTI]

    Nijholt, Anton

    The seven articles in this special issue focus on human computing. Most focus on two challenging issues in human computing, namely, machine analysis of human behavior in group interactions and context-sensitive modeling.

  18. carleton.ca Human Rights

    E-Print Network [OSTI]

    Dawson, Jeff W.

    , including social movements, domestic legislation, international statutes, and human rights declarations the development of historical and contemporary social movements, and the effects of global capitalismcarleton.ca Human Rights #12;Human rights contribute, through theory and practice, to people

  19. Human Reliability Program Overview

    SciTech Connect (OSTI)

    Bodin, Michael

    2012-09-25

    This presentation covers the high points of the Human Reliability Program, including certification/decertification, critical positions, due process, organizational structure, program components, personnel security, an overview of the US DOE reliability program, retirees and academia, and security program integration.

  20. KRFTWRK – Global Human Electricity

    E-Print Network [OSTI]

    Prohaska, Rainer

    2009-01-01

    Power Network 2.1.1 Virtual Power Plants The Global Powernetwork, based on "Virtual Power Plants", called "VPP". A "participant runs a virtual human power plant. Per every "

  1. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-12-20

    The Policy is to establish DOE-specific principles for the protection of human subjects involved in DOE research. Cancels DOE P 443.1. Canceled by DOE O 443.1B

  2. Human-spacesuit interaction :

    E-Print Network [OSTI]

    Hilbert, Alexandra Marie

    2015-01-01

    Extravehicular activities (EVA), or space walks, are a critical and complex aspect of human spaceflight missions. To prepare for safe and successful execution of the required tasks, astronauts undergo extensive training ...

  3. Protection of Human Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2000-05-15

    The purpose of this Policy is to establish DOE-specific policy for the protection of human subjects involved in DOE research. Canceled by DOE P 443.1A.

  4. Human Resource Management Delegation

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1996-06-28

    The notice is to clarifies and updates existing Human Resource Management Delegation Authorities and the levels to which they are delegated. Expired 6-28-97. Does not cancel any directives.

  5. SU-E-J-212: Identifying Bones From MRI: A Dictionary Learnign and Sparse Regression Approach

    SciTech Connect (OSTI)

    Ruan, D; Yang, Y; Cao, M; Hu, P; Low, D [UCLA, Los Angeles, CA (United States)

    2014-06-01

    Purpose: To develop an efficient and robust scheme to identify bony anatomy based on MRI-only simulation images. Methods: MRI offers important soft tissue contrast and functional information, yet its lack of correlation to electron-density has placed it as an auxiliary modality to CT in radiotherapy simulation and adaptation. An effective scheme to identify bony anatomy is an important first step towards MR-only simulation/treatment paradigm and would satisfy most practical purposes. We utilize a UTE acquisition sequence to achieve visibility of the bone. By contrast to manual + bulk or registration-to identify bones, we propose a novel learning-based approach for improved robustness to MR artefacts and environmental changes. Specifically, local information is encoded with MR image patch, and the corresponding label is extracted (during training) from simulation CT aligned to the UTE. Within each class (bone vs. nonbone), an overcomplete dictionary is learned so that typical patches within the proper class can be represented as a sparse combination of the dictionary entries. For testing, an acquired UTE-MRI is divided to patches using a sliding scheme, where each patch is sparsely regressed against both bone and nonbone dictionaries, and subsequently claimed to be associated with the class with the smaller residual. Results: The proposed method has been applied to the pilot site of brain imaging and it has showed general good performance, with dice similarity coefficient of greater than 0.9 in a crossvalidation study using 4 datasets. Importantly, it is robust towards consistent foreign objects (e.g., headset) and the artefacts relates to Gibbs and field heterogeneity. Conclusion: A learning perspective has been developed for inferring bone structures based on UTE MRI. The imaging setting is subject to minimal motion effects and the post-processing is efficient. The improved efficiency and robustness enables a first translation to MR-only routine. The scheme generalizes to multiple tissue classes.

  6. The Evolution of Human Cooperation

    E-Print Network [OSTI]

    Gintis, Herbert; Doebeli, Michael; Flack, Jessica

    2012-01-01

    684 Gintis, H. 2011. The Evolution of Human Cooperation.misunderstandings about cultural evolution. Human Nat. 19,Feldman, M. , 1981. Cultural Evolution. Princeton University

  7. ORISE: Protecting Human Subjects Website

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Protecting Human Subjects Website Institutions that engage in human subjects research are required by federal policy to establish an institutional review board (IRB) to ensure that...

  8. Lung cancer-derived Dickkopf1 is associated with bone metastasis and the mechanism involves the inhibition of osteoblast differentiation

    SciTech Connect (OSTI)

    Chu, Tianqing; Teng, Jiajun; Jiang, Liyan; Zhong, Hua; Han, Baohui

    2014-01-17

    Highlights: •DKK1 level was associated with NSCLC bone metastases. •Lung tumor cells derived DKK1 inhibited osteoblast differentiation. •Lung tumor cells derived DKK1 modulates ?-catenin and RUNX2. -- Abstract: Wnt/?-catenin signaling and Dickkopf1 (DKK1) play important roles in the progression of lung cancer, which preferably metastasizes to skeleton. But the role of them in bone dissemination is poorly understood. This study aims to define the role of DKK1 in lung cancer bone metastases and investigate the underlying mechanism. Our results demonstrated that DKK1 over-expression was a frequent event in non-small-cell lung cancer (NSCLC) blood samples, and serous DKK1 level was much higher in bone metastatic NSCLC compared to non-bone metastatic NSCLC. We also found that conditioned medium from DKK1 over-expressing lung cancer cells inhibited the differentiation of osteoblast, determined by alkaline phosphatase activity and osteocalcin secretion, whereas the conditioned medium from DKK1 silencing lung cancer cells exhibited the opposite effects. Mechanistically, DKK1 reduced the level of ?-catenin and RUNX2, as well as inhibiting the nuclear translocation of ?-catenin. Taken together, these results suggested that lung cancer-produced DKK1 may be an important mechanistic link between NSCLC and bone metastases, and targeting DKK1 may be an effective method to treat bone metastase of NSCLC.

  9. The Human Genome From human genome to other

    E-Print Network [OSTI]

    Linial, Michal

    The Human Genome Project From human genome to other genomes and to gene function June 2000 From genome to health Structural Genomics initiative #12;What is the Human Genome Project? · U.S. govt that arise from genome research #12;The Human Genome Project Project began in 1990 as a $3 billion, 15-year

  10. Policy on Human Subjects Research Policy on Human Subjects

    E-Print Network [OSTI]

    Sridhar, Srinivas

    Policy on Human Subjects Research 10/15/2014 Policy on Human Subjects Research I. Purpose and Scope ethical standards for the protection of human subjects, consistent with the principles of the Nuremberg Code and the Belmont Report. Accordingly, the University has established the Office of Human Subject

  11. Mechanosensitivity and Neural Adaptation in Human Somatosensory System

    E-Print Network [OSTI]

    Venkatesan, Lalit Kumar

    2012-05-31

    the TAC-Cell. Face stimulation invoked neuromagnetic responses reflecting cortical activity in the contralateral primary somatosensory cortex (SI), while hand stimulation resulted in robust contralateral SI and posterior parietal cortex (PPC) activation...

  12. Photoacoustic and ultrasonic probing of bone density in ex-vivo animal tissues The purpose of the proposed research is to develop fundamental physical, instrumental and biological aspects of two novel

    E-Print Network [OSTI]

    Sun, Yu

    (photoacoustic probe) of bones through skin layers under various degrees of demineralization and porosity (void

  13. CONSTRUCTING VIRTUAL HUMAN LIFE SIMULATIONS

    E-Print Network [OSTI]

    Kallmann, Marcelo

    , Virtual Environments, Behavioral Animation, Object Interaction, Python. 1. INTRODUCTION Virtual humanCONSTRUCTING VIRTUAL HUMAN LIFE SIMULATIONS Marcelo Kallmann, Etienne de Sevin and Daniel Thalmann human life simulations. Our main goal is to have virtual human actors living and working autonomously

  14. Human MSH2 protein

    DOE Patents [OSTI]

    de la Chapelle, Albert (Helsingfors, FI); Vogelstein, Bert (Baltimore, MD); Kinzler, Kenneth W. (Baltimore, MD)

    1997-01-01

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error.sup.+ (RER.sup.+) tumor cells.

  15. Human MSH2 protein

    DOE Patents [OSTI]

    Chapelle, A. de la; Vogelstein, B.; Kinzler, K.W.

    1997-01-07

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error{sup +} (RER{sup +}) tumor cells. 19 figs.

  16. Human Genome: DOE Origins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (JournalvivoHigh energyHighlandWorkshop-SummerHow is theHughHumanHuman Genome

  17. Potential commercial application of a bi-layer bone-ligament regeneration scaffold to anterior cruciate ligament replacement

    E-Print Network [OSTI]

    Li, Jessica C. (Jessica Ching-Yi)

    2006-01-01

    A business model was created in order to explore the commercial application of a bi-layer bone-ligament scaffold to the treatment of torn anterior cruciate ligaments (ACL) requiring replacement. The two main keys in producing ...

  18. Regional geologic characterization of the Second Bone Spring Sandstone, Delaware basin, Lea and Eddy Counties, New Mexico 

    E-Print Network [OSTI]

    Downing, Amanda Beth

    2001-01-01

    The Bone Spring Formation is a series of interbedded siliciclastics and carbonates that were deposited in the Delaware basin during the Leonardian (Early Permian). It consists of the First, Second and Third Carbonate and the First, Second and Third...

  19. Pre- and post-contrast three-dimensional double inversion-recovery MRI in human glioblastoma

    E-Print Network [OSTI]

    2013-01-01

    lesions in multiple sclerosis: improved detection with 3Da presenting symptom of multiple sclerosis: a clinical caseof cortical lesions in multiple sclerosis using double

  20. Effect of dietary calcium and phosphorus levels on performance and bone development of large-framed developing boars 

    E-Print Network [OSTI]

    Robinson, Robert Glen

    1975-01-01

    EFFECT OF DIETARY CALCIUM AND PHOSPHORUS LEVELS ON PERFORMANCE AND BONE DEVELOPMENT OF LARGE-FRAMED DEVELOPING BOARS A Thesis by ROBERT GLEN ROBINSON Submitted to the Graduate College of Texas AQ4 University in partial fulfillment... of the requirement for the degree of MASTER OF SCIENCE August 1975 Major Subject. : Animal Science EFFECT OF DIETARY CALCIUM AND PHOSPHORUS LEVELS ON PERFORMANCE AND BONE DEVELOPMENT OF LARGE-~ DEVELOPING BOARS A Thesis by ROBERT GLEN ROBINSON Approved...

  1. The relationship of the long bones of the thoracic and pelvic limbs as indicators of muscling in the beef carcass 

    E-Print Network [OSTI]

    Orts, Frank A

    1959-01-01

    and content hys hsirman of Ccmmitt Head of Department, August, 1959 Ths author is grateful for the interest and help of the staff ueabers of the Departasnts of tuinal Husbandry and Genetics, Sinoere gratitude is expressed to Dr. G. T. King, Assistant... Measurements . ~ 35 19, Group 5I Gross Simple Coefficients of Correlation Between Trimmed Bone Lengths and Carouse Measurements ~ . 36 20. Group 6I Gross Simple Coefficients of Correlation Between Trimmed Bone Lengths and Carcass Measurements . . 36 21...

  2. Intra-arterial Autologous Bone Marrow Cell Transplantation in a Patient with Upper-extremity Critical Limb Ischemia

    SciTech Connect (OSTI)

    Madaric, Juraj; Klepanec, Andrej; Mistrik, Martin; Altaner, Cestmir; Vulev, Ivan

    2013-04-15

    Induction of therapeutic angiogenesis by autologous bone marrow mononuclear cell transplantation has been identified as a potential new option in patients with advanced lower-limb ischemia. There is little evidence of the benefit of intra-arterial cell application in upper-limb critical ischemia. We describe a patient with upper-extremity critical limb ischemia with digital gangrene resulting from hypothenar hammer syndrome successfully treated by intra-arterial autologous bone marrow mononuclear cell transplantation.

  3. Effect of irradiation on bone remodelling and the structural integrity of the vertebral column. Doctoral thesis

    SciTech Connect (OSTI)

    Swenson, K.N.

    1990-01-01

    The effects of therapeutic levels of radiation on the axial properties of the primate vertebral column were studied. Seven male rhesus monkeys (Macaca mulatta) were irradiated with a single does of 1300 cGy to the specific lumbar vertebrae of L2, L3, and L4. Three additional animals served as controls. Radiographs were taken before the radiation treatment and just prior to sacrifice to determine density changes in the bone. The animal subjects were sacrificed 105 days following the radiation exposure. Biomechanical testing was completed on lumbar levels 2 and 3 to identify changes in strength characteristics following radiation treatment. Histomorphometric analysis of lumbar vertebrae level 4 was completed to identify volume and surface density changes as well as cellular changes. Tetracycline, dicarbomethylaminomethyl fluorescein (DCAF), and xylenol orange were used as bone labeling agents to aid in the histomorphometry and to obtain dynamic parameter changes.

  4. SU-E-J-250: A Methodology for Active Bone Marrow Protection for Cervical Cancer Intensity-Modulated Radiotherapy Using 18F-FLT PET/CT Image

    SciTech Connect (OSTI)

    Ma, C; Yin, Y

    2014-06-01

    Purpose: The purpose of this study was to compare a radiation therapy treatment planning that would spare active bone marrow and whole pelvic bone marrow using 18F FLT PET/CT image. Methods: We have developed an IMRT planning methodology to incorporate functional PET imaging using 18F FLT/CT scans. Plans were generated for two cervical cancer patients, where pelvicactive bone marrow region was incorporated as avoidance regions based on the range: SUV>2., another region was whole pelvic bone marrow. Dose objectives were set to reduce the volume of active bone marrow and whole bone marraw. The volumes of received 10 (V10) and 20 (V20) Gy for active bone marrow were evaluated. Results: Active bone marrow regions identified by 18F FLT with an SUV>2 represented an average of 48.0% of the total osseous pelvis for the two cases studied. Improved dose volume histograms for identified bone marrow SUV volumes and decreases in V10(average 18%), and V20(average 14%) were achieved without clinically significant changes to PTV or OAR doses. Conclusion: Incorporation of 18F FLT/CT PET in IMRT planning provides a methodology to reduce radiation dose to active bone marrow without compromising PTV or OAR dose objectives in cervical cancer.

  5. Acculturation in the Upper Middle Missouri Valley as Reflected in Modified Bone Assemblages

    E-Print Network [OSTI]

    Weston, Timothy

    1986-05-01

    Gliders 207 Piercing Tools (Awls ) • 20 Tubes/Beads 208 Expedient Tools 211 Other Tool Categories 214 Horn Core/Frontal Tools. 21 Fleshing Tools 21 Cancellous Tools 7 Fish Hooks 8 Polished Fragments • • 221 Ochre-Stained Bone 22 Ornaments . • 22... Components 251 Big Hidatsa Original Components 4 Condensed Components 25 Lower Hidatsa 257 Sakakawea 260 Big Hidatsa 3 Other Measures of Change •. •. 266 Metal/Stone Modification 26 Predominant Method of Manufacture.... .. 271 Combined Site Assemblages...

  6. Automatic detection of bone fragments in poultry using multi-energy x-rays

    DOE Patents [OSTI]

    Gleason, Shaun S. (Knoxville, TN); Paulus, Michael J. (Knoxville, TN); Mullens, James A. (Knoxville, TN)

    2002-04-09

    At least two linear arrays of x-ray detectors are placed below a conveyor belt in a poultry processing plant. Multiple-energy x-ray sources illuminate the poultry and are detected by the detectors. Laser profilometry is used to measure the poultry thickness as the x-ray data is acquired. The detector readout is processed in real time to detect the presence of small highly attenuating fragments in the poultry, i.e., bone, metal, and cartilage.

  7. ENGINEERING AND HUMAN HEALTH

    E-Print Network [OSTI]

    Saskatchewan, University of

    ENGINEERING AND HUMAN HEALTH: FROM THE INSIDE OUT Engineering research on small scales could have huge health implications College of Engineering U N I V E R S I T Y O F S A S K ATC H E WA N CO L L E G 17 Alumni accolades College of Engineering THOROUGHUNIVERSITY OF SASKATCHEWAN COLLEGE OF ENGINEERING

  8. cAMP-response-element-binding protein positively regulates breast cancer metastasis and subsequent bone destruction

    SciTech Connect (OSTI)

    Son, Jieun; Lee, Jong-Ho; Kim, Ha-Neui; Ha, Hyunil Lee, Zang Hee

    2010-07-23

    Research highlights: {yields} CREB is highly expressed in advanced breast cancer cells. {yields} Tumor-related factors such as TGF-{beta} further elevate CREB expression. {yields} CREB upregulation stimulates metastatic potential of breast cancer cells. {yields} CREB signaling is required for breast cancer-induced bone destruction. -- Abstract: cAMP-response-element-binding protein (CREB) signaling has been reported to be associated with cancer development and poor clinical outcome in various types of cancer. However, it remains to be elucidated whether CREB is involved in breast cancer development and osteotropism. Here, we found that metastatic MDA-MB-231 breast cancer cells exhibited higher CREB expression than did non-metastatic MCF-7 cells and that CREB expression was further increased by several soluble factors linked to cancer progression, such as IL-1, IGF-1, and TGF-{beta}. Using wild-type CREB and a dominant-negative form (K-CREB), we found that CREB signaling positively regulated the proliferation, migration, and invasion of MDA-MB-231 cells. In addition, K-CREB prevented MDA-MB-231 cell-induced osteolytic lesions in a mouse model of cancer metastasis. Furthermore, CREB signaling in cancer cells regulated the gene expression of PTHrP, MMPs, and OPG, which are closely involved in cancer metastasis and bone destruction. These results indicate that breast cancer cells acquire CREB overexpression during their development and that this CREB upregulation plays an important role in multiple steps of breast cancer bone metastasis.

  9. Age and gender specific biokinetic model for strontium in humans

    SciTech Connect (OSTI)

    Shagina, N. B.; Tolstykh, E. I.; Degteva, M. O.; Anspaugh, L. R.; Napier, Bruce A.

    2015-03-01

    A biokinetic model for strontium in humans is necessary for quantification of internal doses due to strontium radioisotopes. The ICRP-recommended biokinetic model for strontium has limitation for use in a population study, because it is not gender specific and does not cover all age ranges. The extensive Techa River data set on 90Sr in humans (tens of thousands of measurements) is a unique source of data on long-term strontium retention for men and women of all ages at intake. These, as well as published data, were used for evaluation of age- and gender-specific parameters for a new compartment biokinetic model for strontium (Sr-AGe model). The Sr-AGe model has similar structure as the ICRP model for the alkaline earth elements. The following parameters were mainly reevaluated: gastro-intestinal absorption and parameters related to the processes of bone formation and resorption defining calcium and strontium transfers in skeletal compartments. The Sr-AGe model satisfactorily describes available data sets on strontium retention for different kinds of intake (dietary and intravenous) at different ages (0–80 years old) and demonstrates good agreement with data sets for different ethnic groups. The Sr-AGe model can be used for dose assessment in epidemiological studies of general population exposed to ingested strontium radioisotopes.

  10. Texas Tech University Human Resources

    E-Print Network [OSTI]

    Rock, Chris

    to redevelop Texas Tech University's human resource functions into a high-performing human capital managementTexas Tech University Human Resources Strategic Plan January 1, 2014 - December 31, 2016 #12;2 Mission Texas Tech University is recognized as a premier institution and a workplace of choice. This work

  11. Understanding Human Experience Henry Kautz

    E-Print Network [OSTI]

    Kautz, Henry

    Understanding Human Experience Henry Kautz One of the earliest goals of research in artificial intelligence was to create systems that can interpret and understand day to day human experience. Early work on the goal of building systems that understand human experience. Each of the previous barriers is weakened

  12. Human Capital Management Accountability Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2008-08-01

    The Order establishes requirements, roles and responsibilities for the Human Capital Management Accountability Program (HCMAP) for human resources programs and personnel and ensures that human capital activities are regulatory and procedurally compliant with Federal statutes and Departmental policies. Does not cancel other directives.

  13. Morphology and development of the human vocal tract: A study using magnetic resonance imaging

    E-Print Network [OSTI]

    Fitch, Tecumseh

    which have generally focused on bony struc- tures vertebral column, skull base, hyoid bone, etc. , MRI

  14. Enhancement of bone marrow allografts from nude mice into mismatched recipients by T cells void of graft-versus-host activity

    SciTech Connect (OSTI)

    Lapidot, T.; Lubin, I.; Terenzi, A.; Faktorowich, Y.; Erlich, P.; Reisner, Y. (Weizmann Institute of Science, Rehovot (Israel))

    1990-06-01

    Transplantation of 8 x 10(6) C57BL/6-Nu+/Nu+ (nude) bone marrow cells into C3H/HeJ recipients after conditioning with 8 Gy of total body irradiation has resulted in a markedly higher rate of graft rejection or graft failure compared to that found in recipients of normal C57BL/6 or C57BL/6-Bg+/Bg+ (beige) T-cell-depleted bone marrow. Mixing experiments using different numbers of nude bone marrow cells with or without mature thymocytes (unagglutinated by peanut agglutinin) revealed that engraftment of allogeneic T-cell-depleted bone marrow is T-cell dependent. To ensure engraftment, a large inoculum of nude bone marrow must be supplemented with a trace number of donor T cells, whereas a small bone marrow dose from nude donors requires a much larger number of T cells for engraftment. Marked enhancement of donor type chimerism was also found when F1 thymocytes were added to nude bone marrow cells, indicating that the enhancement of bone marrow engraftment by T cells is not only mediated by alloreactivity against residual host cells but may rather be generated by growth factors, the release of which may require specific interactions between T cells and stem cells or between T cells and bone marrow stroma cells.

  15. Sequential Causal Learning in Humans and Rats

    E-Print Network [OSTI]

    Lu, Hongjing; Rojas, Randall R.; Beckers, Tom; Yuille, Alan

    2008-01-01

    selection, to a human experiment that employed pretraining (group (white) in human experiment by Beckers et al. (2005).set used for the human experiments, we increased the

  16. Sequential Causal Learning in Humans and Rats

    E-Print Network [OSTI]

    Hongjing Lu; Randall R. Rojas; Tom Beckers; Alan Yuille

    2011-01-01

    selection, to a human experiment that employed pretraining (group (white) in human experiment by Beckers et al. (2005).set used for the human experiments, we increased the

  17. Allele-specific gene regulation in humans

    E-Print Network [OSTI]

    Maynard, Nathaniel David

    2008-01-01

    1 Introduction The Human Genome Project has provided thefrom clones and the human genome project have revealed thatVariation The Human Genome Project provided scientists with

  18. Project ATHENA creates surrogate human organ systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Project ATHENA creates surrogate human organ systems Project ATHENA creates surrogate human organ systems The development of miniature surrogate human organs, coupled with highly...

  19. Timescales of Massive Human Entrainment

    E-Print Network [OSTI]

    Fusaroli, Riccardo; Mislove, Alan; Paxton, Alexandra; Matlock, Teenie; Dale, Rick

    2014-01-01

    The past two decades have seen an upsurge of interest in the collective behaviors of complex systems composed of many agents. In this paper, we extend concepts of entrainment to the dynamics of human collective attention. We demonstrate that large scale human entrainment may hold across a number of distinct scales, in an exquisitely time locked fashion. Using a large scale database of human communication data, we analyze and describe three different time scales of human entrainment in electronic media. We sought a distinct shared experience that provided a test bed for quantifying large scale human entrainment. We conducted a detailed investigation of the real time unfolding of human entrainment, as expressed by the content and patterns of hundreds of thousands of messages on Twitter, during the 2012 US presidential debates. By time locking these data sources, we quantify the real time impact of the debate on human attention. We show that social behavior covaries second by second to the interactional dynamics...

  20. Human Genome Program

    SciTech Connect (OSTI)

    Not Available

    1993-01-01

    The DOE Human Genome program has grown tremendously, as shown by the marked increase in the number of genome-funded projects since the last workshop held in 1991. The abstracts in this book describe the genome research of DOE-funded grantees and contractors and invited guests, and all projects are represented at the workshop by posters. The 3-day meeting includes plenary sessions on ethical, legal, and social issues pertaining to the availability of genetic data; sequencing techniques, informatics support; and chromosome and cDNA mapping and sequencing.

  1. HUMAN MACHINE COOPERATIVE TELEROBOTICS

    SciTech Connect (OSTI)

    William R. Hamel; Spivey Douglass; Sewoong Kim; Pamela Murray; Yang Shou; Sriram Sridharan; Ge Zhang; Scott Thayer; Rajiv V. Dubey

    2003-06-30

    The remediation and deactivation and decommissioning (D&D) of nuclear waste storage tanks using telerobotics is one of the most challenging tasks faced in environmental cleanup. Since a number of tanks have reached the end of their design life and some of them have leaks, the unstructured, uncertain and radioactive environment makes the work inefficient and expensive. However, the execution time of teleoperation consumes ten to hundred times that of direct contact with an associated loss in quality. Thus, a considerable effort has been expended to improve the quality and efficiency of telerobotics by incorporating into teleoperation and robotic control functions such as planning, trajectory generation, vision, and 3-D modeling. One example is the Robot Task Space Analyzer (RTSA), which has been developed at the Robotics and Electromechanical Systems Laboratory (REMSL) at the University of Tennessee in support of the D&D robotic work at the Oak Ridge National Laboratory and the National Energy Technology Laboratory. This system builds 3-D models of the area of interest in task space through automatic image processing and/or human interactive manual modeling. The RTSA generates a task plan file, which describes the execution of a task including manipulator and tooling motions. The high level controller of the manipulator interprets the task plan file and executes the task automatically. Thus, if the environment is not highly unstructured, a tooling task, which interacts with environment, will be executed in the autonomous mode. Therefore, the RTSA not only increases the system efficiency, but also improves the system reliability because the operator will act as backstop for safe operation after the 3-D models and task plan files are generated. However, unstructured conditions of environment and tasks necessitate that the telerobot operates in the teleoperation mode for successful execution of task. The inefficiency in the teleoperation mode led to the research described as Human Machine Cooperative Telerobotics (HMCTR). The HMCTR combines the telerobot with robotic control techniques to improve the system efficiency and reliability in teleoperation mode. In this topical report, the control strategy, configuration and experimental results of Human Machines Cooperative Telerobotics (HMCTR), which modifies and limits the commands of human operator to follow the predefined constraints in the teleoperation mode, is described. The current implementation is a laboratory-scale system that will be incorporated into an engineering-scale system at the Oak Ridge National Laboratory in the future.

  2. Human portable preconcentrator system

    DOE Patents [OSTI]

    Linker, Kevin L. (Albuquerque, NM); Bouchier, Francis A. (Albuquerque, NM); Hannum, David W. (Albuquerque, NM); Rhykerd, Jr., Charles L. (Albuquerque, NM)

    2003-01-01

    A preconcentrator system and apparatus suited to human portable use wherein sample potentially containing a target chemical substance is drawn into a chamber and through a pervious screen. The screen is adapted to capture target chemicals and then, upon heating, to release those chemicals into the chamber. Chemicals captured and then released in this fashion are then carried to a portable chemical detection device such as a portable ion mobility spectrometer. In the preferred embodiment, the means for drawing sample into the chamber comprises a reversible fan which, when operated in reverse direction, creates a backpressure that facilitates evolution of captured target chemicals into the chamber when the screen is heated.

  3. ORISE: Protecting Human Subjects

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesseworkSURVEY UNIVERSE TheForensicPerformanceProtecting Human Subjects

  4. Influence of zinc on growth and bone maturation in children with end stage renal disease (ESRD)

    SciTech Connect (OSTI)

    Hagan, D.; Fleischmann, L.; Schemmel, R.A.

    1986-03-05

    Children with ESRD, age 5-19 years, were supplemented with zinc acetate (2 mg/kg BW, maximum 40 mg/da/child) to determine if zinc supplements (ZS) would improve growth and bone maturation. Twelve children completed the study. Three of the 12 did not receive ZS. Seven of the 9 ZS children were followed for 1 year pre- and 1 yr during-ZS. Two subjects were followed for shorter periods of time. Heights, weights, and hand wrist radiographs were taken at the beginning of the study, just pre-ZS, and at the end of the study. Blood was analyzed for serum alkaline phosphatase and albumin monthly. Alkaline phosphatase was elevated in 7 of 12 subjects pre-ZS and in 5 of 9 subjects post-ZS. Albumin levels were below normal in 7 subjects pre-ZS and 4 subjects post-ZS. Mean plasma Zn and Cu levels, 97+/-17 and 164+/-42 mcg/dl, pre-ZS, and 102+/-30 and 173+/-46 mcg/dl post-ZS, respectively, were similar. Growth velocity in males (4.1+/-2.2 cm/yr, 3.0+/-2.3 cm/yr) and females (3.9+/-0.7, 3.3+/-2.1 cm/yr) pre- and post-ZS, respectively, were similar. Bone maturation per chronological age improved after ZS in 4 of 6 subjects, 1 matured at the same rate, and one at a slower rate. It appears that ZS of children with ESRD increased the rate of bone maturation but not linear growth.

  5. Long bone growth of Mimbres subadults from the NAN Ranch (LA15049), New Mexico 

    E-Print Network [OSTI]

    Marek, Marianne

    1990-01-01

    of 559 skeletons from three time periods (Late Woodland, Mississippian Acculturated Late Woodland, and Table 2. 2. Indian Knoll maximum long bone length (cm) without epiphysis*. 10 13 14 15 16 17 18 2. 5 ? 3. 5 yr. 3. 5 ? 4. 5 yr. 4. 5 ? 5. 5... yr. 5. 5 ? 6. 5 yr. 6. 5 ? 8 yr. 8 ? 10. 5 yr. 10. 5 ? 11 yr. 11 ? 12 yr. 12 yl ~ 15 yr. + 6 mo. 15 yr. 16 yz' ~ 16. 5 ? 17 yr. 21 yr ~ Age Group Age 1 Birth ? 6 mo. 6 ? 15 mo. 15 ? 24 mo. 4 2 ? 2. 5 yr. Humerus N Mean SD Range 5...

  6. PIA - Human Resources - Personal Information Change Request ...

    Energy Savers [EERE]

    Human Resources - Personal Information Change Request - Idaho National Engineering Laboratory PIA - Human Resources - Personal Information Change Request - Idaho National...

  7. Human subjects research handbook: Protecting human research subjects. Second edition

    SciTech Connect (OSTI)

    NONE

    1996-01-30

    This handbook serves as a guide to understanding and implementing the Federal regulations and US DOE Orders established to protect human research subjects. Material in this handbook is directed towards new and continuing institutional review board (IRB) members, researchers, institutional administrators, DOE officials, and others who may be involved or interested in human subjects research. It offers comprehensive overview of the various requirements, procedures, and issues relating to human subject research today.

  8. Property CV3 16:06 02/06/2006 1 Variability of the mechanical properties of bone, and its evolutionary

    E-Print Network [OSTI]

    Baxter, Paul D.

    properties; evolutionary optima 1. INTRODUCTION The objects of the work described in this paper mechanical properties of bone: Young's modulus, strain at failure, impact energy absorption and so on cannot insights may often be gained by doing so. For instance, except for very highly mineralised bone, bending

  9. Reactive oxygen species on bone mineral density and mechanics in Cu,Zn superoxide dismutase (Sod1) knockout mice

    SciTech Connect (OSTI)

    Smietana, Michael J. [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States)] [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States); Arruda, Ellen M. [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States) [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States); Mechanical Engineering, University of Michigan, 2250 GG Brown, 2350 Hayward, Ann Arbor, MI 48109 (United States); Program in Macromolecular Science and Engineering, University of Michigan, 2250 GG Brown, 2350 Hayward, Ann Arbor, MI 48109 (United States); Faulkner, John A.; Brooks, Susan V. [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States) [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States); Molecular and Integrative Physiology, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States); Larkin, Lisa M., E-mail: llarkin@umich.edu [Biomedical Engineering, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States); Molecular and Integrative Physiology, University of Michigan, 2025 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200 (United States)

    2010-12-03

    Research highlights: {yields} Reactive oxygen species (ROS) are considered to be a factor in the onset of a number of age-associated conditions, including loss of BMD. {yields} Cu,Zn-superoxide dismutase (Sod1) deficient mice have increased ROS, reduced bone mineral density, decreased bending stiffness, and decreased strength compared to WT controls. {yields} Increased ROS caused by the deficiency of Sod1, may be responsible for the changes in BMD and bone mechanics and therefore represent an appropriate model for studying mechanisms of age-associated bone loss. -- Abstract: Reactive oxygen species (ROS) play a role in a number of degenerative conditions including osteoporosis. Mice deficient in Cu,Zn-superoxide dismutase (Sod1) (Sod1{sup -/-} mice) have elevated oxidative stress and decreased muscle mass and strength compared to wild-type mice (WT) and appear to have an accelerated muscular aging phenotype. Thus, Sod1{sup -/-} mice may be a good model for evaluating the effects of free radical generation on diseases associated with aging. In this experiment, we tested the hypothesis that the structural integrity of bone as measured by bending stiffness (EI; N/mm{sup 2}) and strength (MPa) is diminished in Sod1{sup -/-} compared to WT mice. Femurs were obtained from male and female WT and Sod1{sup -/-} mice at 8 months of age and three-point bending tests were used to determine bending stiffness and strength. Bones were also analyzed for bone mineral density (BMD; mg/cc) using micro-computed tomography. Femurs were approximately equal in length across all groups, and there were no significant differences in BMD or EI with respect to gender in either genotype. Although male and female mice demonstrated similar properties within each genotype, Sod1{sup -/-} mice exhibited lower BMD and EI of femurs from both males and females compared with gender matched WT mice. Strength of femurs was also lower in Sod1{sup -/-} mice compared to WT as well as between genders. These data indicate that increased oxidative stress, due to the deficiency of Sod1 is associated with decreased bone stiffness and strength and Sod1{sup -/-} mice may represent an appropriate model for studying disease processes in aging bone.

  10. Trichodermin induces cell apoptosis through mitochondrial dysfunction and endoplasmic reticulum stress in human chondrosarcoma cells

    SciTech Connect (OSTI)

    Su, Chen-Ming; Wang, Shih-Wei; Lee, Tzong-Huei; Tzeng, Wen-Pei; Hsiao, Che-Jen; Liu, Shih-Chia; Tang, Chih-Hsin

    2013-10-15

    Chondrosarcoma is the second most common primary bone tumor, and it responds poorly to both chemotherapy and radiation treatment. Nalanthamala psidii was described originally as Myxosporium in 1926. This is the first study to investigate the anti-tumor activity of trichodermin (trichothec-9-en-4-ol, 12,13-epoxy-, acetate), an endophytic fungal metabolite from N. psidii against human chondrosarcoma cells. We demonstrated that trichodermin induced cell apoptosis in human chondrosarcoma cell lines (JJ012 and SW1353 cells) instead of primary chondrocytes. In addition, trichodermin triggered endoplasmic reticulum (ER) stress protein levels of IRE1, p-PERK, GRP78, and GRP94, which were characterized by changes in cytosolic calcium levels. Furthermore, trichodermin induced the upregulation of Bax and Bid, the downregulation of Bcl-2, and the dysfunction of mitochondria, which released cytochrome c and activated caspase-3 in human chondrosarcoma. In addition, animal experiments illustrated reduced tumor volume, which led to an increased number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive cells and an increased level of cleaved PARP protein following trichodermin treatment. Together, this study demonstrates that trichodermin is a novel anti-tumor agent against human chondrosarcoma cells both in vitro and in vivo via mitochondrial dysfunction and ER stress. - Highlights: • Trichodermin induces chondrosarcoma apoptosis. • ER stress is involved in trichodermin-induced cell death. • Trichodermin induces chondrosarcoma death in vivo.

  11. Human portable preconcentrator system

    DOE Patents [OSTI]

    Linker, Kevin L.; Brusseau, Charles A.; Hannum, David W.; Puissant, James G.; Varley, Nathan R.

    2003-08-12

    A preconcentrator system and apparatus suited to human portable use wherein sample potentially containing a target chemical substance is drawn into a chamber and through a pervious screen. The screen is adapted to capture target chemicals and then, upon heating, to release those chemicals into the chamber. Chemicals captured and then released in this fashion are then carried to a portable chemical detection device such as a portable ion mobility spectrometer. In the preferred embodiment, the means for drawing sample into the chamber comprises a reversible fan which, when operated in reverse direction, creates a backpressure that facilitates evolution of captured target chemicals into the chamber when the screen is heated. The screen can be positioned directly in front of the detector prior to heating to improve detection capability.

  12. Human-computer interface

    DOE Patents [OSTI]

    Anderson, Thomas G.

    2004-12-21

    The present invention provides a method of human-computer interfacing. Force feedback allows intuitive navigation and control near a boundary between regions in a computer-represented space. For example, the method allows a user to interact with a virtual craft, then push through the windshield of the craft to interact with the virtual world surrounding the craft. As another example, the method allows a user to feel transitions between different control domains of a computer representation of a space. The method can provide for force feedback that increases as a user's locus of interaction moves near a boundary, then perceptibly changes (e.g., abruptly drops or changes direction) when the boundary is traversed.

  13. Human Reliability Program Workshop

    SciTech Connect (OSTI)

    Landers, John; Rogers, Erin; Gerke, Gretchen

    2014-05-18

    A Human Reliability Program (HRP) is designed to protect national security as well as worker and public safety by continuously evaluating the reliability of those who have access to sensitive materials, facilities, and programs. Some elements of a site HRP include systematic (1) supervisory reviews, (2) medical and psychological assessments, (3) management evaluations, (4) personnel security reviews, and (4) training of HRP staff and critical positions. Over the years of implementing an HRP, the Department of Energy (DOE) has faced various challenges and overcome obstacles. During this 4-day activity, participants will examine programs that mitigate threats to nuclear security and the insider threat to include HRP, Nuclear Security Culture (NSC) Enhancement, and Employee Assistance Programs. The focus will be to develop an understanding of the need for a systematic HRP and to discuss challenges and best practices associated with mitigating the insider threat.

  14. Editorial: The Human Genome Project

    E-Print Network [OSTI]

    Crawford, Michael H.; Baer, A.S.; Hall, R.; Omenn, G.S.; Thomson, G.J.; Wilson, A.C.

    1990-08-01

    iv / Editorial: The Human Genome Project Dear readers, The last few decades have seen a number of exciting developments in genetics. First, Watson and Crick broke the genetic code; since then, tech-nologic and methodologic breakthroughs have... permitted the study and direct manipulation of our DNA. Now there is an international ground swell to map and sequence the human genome. The Bush administration had originally requested $128 million in last year's budget for the Human Genome Project. However...

  15. Protection of Human Research Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2015-07-20

    Changes are made to harmonize the definitions in this Order with those in the Federal regulations for the protection of human subjects (10 CFR Part 745), specifically, splitting the definition "human subject research" into "research" and "human subject," and adopting, verbatim, the definitions of "research" and "human subject" from 10 CFR Part 745 and adding the definition of "generalizable," since the determination of whether a project is "research" in 10 CFR Part 745 hinges on whether the work being conducted is generalizable. Small corrections and updates have been made to the references, links, and organization titles.

  16. Quantum physics and human values

    SciTech Connect (OSTI)

    Stapp, H.P.

    1989-09-01

    This report discusses the following concepts: the quantum conception of nature; the quantum conception of man; and the impact upon human values. (LSP).

  17. ORISE: Human Subjects Research Database

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in support of the HSRD database: Database maintenance Federal Internet server access Software development Quality assurancequality control Project assistance Human Subjects...

  18. Integrated design environment for human performance and human reliability analysis

    SciTech Connect (OSTI)

    Nelson, W.R.

    1997-05-01

    Work over the last few years at the Idaho National Engineering and Environmental Laboratory (INEEL) has included a major focus on applying human performance and human reliability knowledge and methods as an integral element of system design and development. This work has been pursued in programs in a wide variety of technical domains, beginning with nuclear power plant operations. Since the mid-1980`s the laboratory has transferred the methods and tools developed in the nuclear domain to military weapons systems and aircraft, offshore oil and shipping operations, and commercial aviation operations and aircraft design. Through these diverse applications the laboratory has developed an integrated approach and framework for application of human performance analysis, human reliability analysis (HRA), operational data analysis, and simulation studies of human performance to the design and development of complex systems. This approach was recently tested in the NASA Advanced Concepts Program {open_quotes}Structured Human Error Analysis for Aircraft Design.{close_quotes} This program resulted in the prototype software tool THEA (Tool for Human Error Analysis) for incorporating human error analysis in the design of commercial aircraft, focusing on airplane maintenance tasks. Current effort is directed toward applying this framework to the development of advanced Air Traffic Management (ATM) systems as part of NASA`s Advanced Air Transportation Technologies (AATT) program. This paper summarizes the approach, describes recent and current applications in commercial aviation, and provides perspectives on how the approach could be utilized in the nuclear power industry.

  19. Simulating human behavior for national security human interactions.

    SciTech Connect (OSTI)

    Bernard, Michael Lewis; Hart, Dereck H.; Verzi, Stephen J.; Glickman, Matthew R.; Wolfenbarger, Paul R.; Xavier, Patrick Gordon

    2007-01-01

    This 3-year research and development effort focused on what we believe is a significant technical gap in existing modeling and simulation capabilities: the representation of plausible human cognition and behaviors within a dynamic, simulated environment. Specifically, the intent of the ''Simulating Human Behavior for National Security Human Interactions'' project was to demonstrate initial simulated human modeling capability that realistically represents intra- and inter-group interaction behaviors between simulated humans and human-controlled avatars as they respond to their environment. Significant process was made towards simulating human behaviors through the development of a framework that produces realistic characteristics and movement. The simulated humans were created from models designed to be psychologically plausible by being based on robust psychological research and theory. Progress was also made towards enhancing Sandia National Laboratories existing cognitive models to support culturally plausible behaviors that are important in representing group interactions. These models were implemented in the modular, interoperable, and commercially supported Umbra{reg_sign} simulation framework.

  20. History of Humanities Associate Editors

    E-Print Network [OSTI]

    Mateo, Jill M.

    History of Humanities Associate Editors Rick Altman, Film Studies, University of Iowa Carolyn History of Science and Humanities, Utrecht University David Cram, History of Linguistics, University, Max Planck Institute for the History of Science Caroline van Eck, Art and Architecture, Leiden

  1. The human activity of visualization

    E-Print Network [OSTI]

    Wright, Dawn Jeannine

    Griffin et al 2006 #12;Human-Computer Interaction: Software of the Mind each user has a setThe human activity of visualization cultural and psychological factors in representation; Gibbon 1998; Marcus 2000) conventions and metaphors of Westerners may not hold worldwide colors

  2. The Human Genome Diversity Project

    SciTech Connect (OSTI)

    Cavalli-Sforza, L.

    1994-12-31

    The Human Genome Diversity Project (HGD Project) is an international anthropology project that seeks to study the genetic richness of the entire human species. This kind of genetic information can add a unique thread to the tapestry knowledge of humanity. Culture, environment, history, and other factors are often more important, but humanity`s genetic heritage, when analyzed with recent technology, brings another type of evidence for understanding species` past and present. The Project will deepen the understanding of this genetic richness and show both humanity`s diversity and its deep and underlying unity. The HGD Project is still largely in its planning stages, seeking the best ways to reach its goals. The continuing discussions of the Project, throughout the world, should improve the plans for the Project and their implementation. The Project is as global as humanity itself; its implementation will require the kinds of partnerships among different nations and cultures that make the involvement of UNESCO and other international organizations particularly appropriate. The author will briefly discuss the Project`s history, describe the Project, set out the core principles of the Project, and demonstrate how the Project will help combat the scourge of racism.

  3. Poly(e-caprolactone) Composites Containing Gentamicin-Loaded b-Tricalcium Phosphate/Gelatin Microspheres as Bone Tissue Supports

    E-Print Network [OSTI]

    Hasýrcý, Vasýf

    Poly(e-caprolactone) Composites Containing Gentamicin-Loaded b-Tricalcium Phosphate/Gelatin-tricalcium phosphate (b-TCP) and gelatin. The purpose is to use this biode- gradable material as a support for bone. The effects of the ratio of the b-TCP/gelatin microspheres on the morphological, mechanical, and degradation

  4. Further evaluation of the efficacy of contamination removal from bone Jodi Lynn Barta a,b,c

    E-Print Network [OSTI]

    Kemp, Brian M.

    Further evaluation of the efficacy of contamination removal from bone surfaces Jodi Lynn Barta a, it simultaneously represents a system that is hypersen- sitive to amplifying contaminant DNA [7­9]. Tempering) and degraded DNA are prone to contamination from exogenous DNA sources that in some cases can out

  5. The effect of alcohol on the bone growth spurt of rats at a time equivalent to adolescent females 

    E-Print Network [OSTI]

    Chaffin, Catherine Lee

    1997-01-01

    2, 4, 6 and 8 weeks after the feeding began. I-Estomorphometric analyses were performed using a BioQuant Morphometric system on 5um undecalcified longitudinal sections of the proximal tibia. A decrease in the amount of trabecular bone was found...

  6. Genetic Inactivation of ERK1 and ERK2 in Chondrocytes Promotes Bone Growth and Enlarges the Spinal Canal

    E-Print Network [OSTI]

    Genetic Inactivation of ERK1 and ERK2 in Chondrocytes Promotes Bone Growth and Enlarges the Spinal dysplasia. In mouse models of achondroplasia, recent studies have implicated the ERK MAPK pathway, a pathway and ERK MAPK signaling in chondrocytes also causes premature synchondrosis closure in the cranial base

  7. A new technique for imaging Mineralized Fibrils on Bovine Trabecular Bone Fracture Surfaces by Atomic Force Microscopy

    E-Print Network [OSTI]

    Hansma, Paul

    A new technique for imaging Mineralized Fibrils on Bovine Trabecular Bone Fracture Surfaces coated with extrafibrillar mineral particles. The mineral particles are distinctly different in different collagen fibrils. If the observed particles can be verified to be native extrafibrillar mineral, this could

  8. Fracture Mechanisms of Bone: A Comparative Study between Antler and Bovine Femur , F.A. Sheppard2

    E-Print Network [OSTI]

    McKittrick, Joanna

    Fracture Mechanisms of Bone: A Comparative Study between Antler and Bovine Femur P.Y. Chen1 , F, University of California, San Diego, La Jolla, CA 92093-0411, U.S.A. ABSTRACT In this study, fracture conditions to study the effects of fiber orientation and hydration. Fracture toughness results

  9. Anti-CD45 radioimmunotherapy using 211At with bone marrow transplantation prolongs survival in a disseminated murine leukemia model

    SciTech Connect (OSTI)

    Orozco, Johnnie J.; Back, Tom; Kenoyer, Aimee L.; Balkin, Ethan R.; Hamlin, Donald K.; Wilbur, D. Scott; Fisher, Darrell R.; Frayo, Shani; Hylarides, Mark; Green, Damian J.; Gopal, Ajay K.; Press, Oliver W.; Pagel, John M.

    2013-05-15

    Anti-CD45 Radioimmunotherapy using an Alpha-Emitting Radionuclide 211At Combined with Bone Marrow Transplantation Prolongs Survival in a Disseminated Murine Leukemia Model ABSTRACT Despite aggressive chemotherapy combined with hematopoietic cell transplant (HCT), many patients with acute myeloid leukemia (AML) relapse. Radioimmunotherapy (RIT) using antibodies (Ab) labeled primarily with beta-emitting radionuclides has been explored to reduce relapse.

  10. Purification of Matrix Gla Protein From a Marine Teleost Fish, Argyrosomus regius: Calcified Cartilage and Not Bone

    E-Print Network [OSTI]

    Price, Paul A.

    Purification of Matrix Gla Protein From a Marine Teleost Fish, Argyrosomus regius: Calcified Cartilage and Not Bone as the Primary Site of MGP Accumulation in Fish DC SIMES,1 MK WILLIAMSON,2 JB ORTIZ to accumulate in vertebra of shark, a cartilaginous fish. However, to date, no information is available on sites

  11. Human retinoblastoma gene

    SciTech Connect (OSTI)

    Bookstein, R.; Lee, E.Y.H.P.; Peccei, A.; Lee, W.H. (Dept. of Pathology M-012 and Center for Molecular Genetics, Univ. of California, San Diego, La Jolla, CA (US))

    1989-04-01

    Mutational inactivation of the retinoblastoma (RB) gene is considered a fundamental event in the formation of several types of human cancer. A substantial proportion of RB gene mutations are partial or complete deletions that extend an unknown distance beyond one or both ends of the gene. To provide a framework for measuring the extent of these deletions, the authors have constructed a long-range restriction map of SfiI sites spanning 850 kilobases around the RB gene. This map was applied in a molecular analysis of RB gene deletion in breast cancer cell line MB468. A previous study of this cell line demonstrated deletion of the entire RB gene except for exons 1 and 2. Genomic clones containing the deletion junction were isolated from a library made from MB468 DNA. A probe obtained from the far side of the deletion junction was used to localize and clone the unknown 3' endpoint, demonstrating that the chromosomal mutation in this case was a simple deletion spanning 200 kilobases. Sequence analysis of the deletion junction indicated a conservative deletion with no loss or gain of nucleotides. The deletion endpoints had no sequence homology to each other or to any repetitive sequence family, such as Alu, so the recombination event was illegitimate. Structural analysis of this and other RB gene deletions is important for understanding molecular mechanisms of recessive oncogenesis.

  12. Reconstructing Cortical Dynamics with Magnetoencephalography

    E-Print Network [OSTI]

    Dalal, Sarang S.

    2007-01-01

    ciently on modern high performance computing clusters. Thiswell-suited to run on high performance computing clusters. Savailability of high performance computing clusters (e.g. ,

  13. Biomechanics of single cortical neurons

    E-Print Network [OSTI]

    Bernick, Kristin B.

    This study presents experimental results and computational analysis of the large strain dynamic behavior of single neurons in vitro with the objective of formulating a novel quantitative framework for the biomechanics of ...

  14. Reconstructing Cortical Dynamics with Magnetoencephalography

    E-Print Network [OSTI]

    Dalal, Sarang S.

    2007-01-01

    an event-related potential (ERP) in the case of EEG or anof synchrony necessary for the ERP/ERF model of activation (necessarily noise, the average ERP/ERF is not necessarily

  15. Human Reliability Analysis for Digital Human-Machine Interfaces

    SciTech Connect (OSTI)

    Ronald L. Boring

    2014-06-01

    This paper addresses the fact that existing human reliability analysis (HRA) methods do not provide guidance on digital human-machine interfaces (HMIs). Digital HMIs are becoming ubiquitous in nuclear power operations, whether through control room modernization or new-build control rooms. Legacy analog technologies like instrumentation and control (I&C) systems are costly to support, and vendors no longer develop or support analog technology, which is considered technologically obsolete. Yet, despite the inevitability of digital HMI, no current HRA method provides guidance on how to treat human reliability considerations for digital technologies.

  16. Liquid-Solid Phase Transition Alloy as Reversible and Rapid Molding Bone Cement

    E-Print Network [OSTI]

    Yi, Liting; Liu, Jing

    2013-01-01

    Bone cement has been demonstrated as an essential restorative material in the orthopedic surgery. However current materials often imply unavoidable drawbacks, such as tissue-cement reaction induced thermal injuries and troublesome revision procedure. Here we proposed an injectable alloy cement to address such problems through its liquid-solid phase transition mechanism. The cement is made of a unique alloy BiInSnZn with a specifically designed low melting point 57.5{\\deg}C. This property enables its rapid molding into various shapes with high plasticity. Some fundamental characteristics including mechanical strength behaviors and phase transition-induced thermal features have been measured to demonstrate the competence of alloy as unconventional cement with favorable merits. Further biocompatible tests showed that this material could be safely employed in vivo. In addition, experiments also found the alloy cement capability as an excellent contrast agent for radiation imaging. Particularly, the proposed alloy...

  17. Abrogation of hybrid resistance to bone marrow engraftment by graft versus host induced immune deficiency

    SciTech Connect (OSTI)

    Hakim, F.T.; Shearer, G.M.

    1986-03-01

    Lethally irradiated F/sub 1/ mice, heterozygous at the hematopoietic histocompatibility (Hh) locus at H-2D/sup b/, reject bone marrow grafts from homozygous H-2/sup b/ parents. This hybrid resistance (HR) is reduced by prior injection of H-2/sup b/ parental spleen cells. Since injection of parental spleen cells produces a profound suppression of F/sub 1/ immune functions, the authors investigated whether parental-induced abrogation of HR was due to graft-vs-host induced immune deficiency (GVHID). HR was assessed by quantifying engraftment in irradiated mice using /sup 125/I-IUdR spleen uptake; GVHID by measuring generation of cytotoxic T lymphocytes (CTL) from unirradiated mice. They observed correlation in time course, spleen dose dependence and T cell dependence between GVHID and loss of HR. The injection of B10 recombinant congenic spleens into (B10 x B10.A) F/sub 1/ mice, prior to grafting with B10 marrow, demonstrated that only those disparities in major histocompatibility antigens which generated GVHID would result in loss of HR. Spleens from (B10 x B10.A(2R))F/sub 1/ mice (Class I disparity only) did not induce GVHID or affect HR, while (B10 x B10.A(5R)F/sub 1/ spleens (Class I and II disparity) abrogated CTL generation and HR completely. GVHID produced by a Class II only disparity, as in (B10 x B10.A(5R))F/sub 1/ spleens injected into (B6/sup bm12 x B10.A(5R))F/sub 1/ mice, was also sufficient to markedly reduce HR to B10 bone marrow. Modulation of hematopoietic graft rejection by GVHID may affect marrow engraftment in man.

  18. Human genome. 1993 Program report

    SciTech Connect (OSTI)

    Not Available

    1994-03-01

    The purpose of this report is to update the Human Genome 1991-92 Program Report and provide new information on the DOE genome program to researchers, program managers, other government agencies, and the interested public. This FY 1993 supplement includes abstracts of 60 new or renewed projects and listings of 112 continuing and 28 completed projects. These two reports, taken together, present the most complete published view of the DOE Human Genome Program through FY 1993. Research is progressing rapidly toward 15-year goals of mapping and sequencing the DNA of each of the 24 different human chromosomes.

  19. Personnel / Human Resource Management MGTK 302 Online

    E-Print Network [OSTI]

    Diestel, Geoff

    competencies: Strategic Human Resource Management: Knowledge of strategic management concepts (strategyPersonnel / Human Resource Management MGTK 302 Online Fall 2014 YOUR INSTRUCTOR Name: Ms. Amanda of human resource management; relationship between personnel management and organizations' emerging role

  20. Essays on Human Development and Public Policy

    E-Print Network [OSTI]

    Raykar, Neha

    2011-01-01

    Statistics released by the Ministry of Human Resource Development of IndiaStatistics released by the Ministry of Human Resource Development of India.India (1991), and National Human Development Report (2001). The descriptive statistics

  1. Engineering humanized mice for improved hematopoietic reconstitution

    E-Print Network [OSTI]

    Drake, Adam

    Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the ...

  2. Human rights: in the real world 

    E-Print Network [OSTI]

    Brown, Abbe

    2006-01-01

    An analysis of the relevance of human rights to litigation and exploitation of intellectual property rights in the UK. The paper considers the impact of the Human Rights Act 1998, and other human rights instruments, ...

  3. Guest editorial: Special issue on human computing

    E-Print Network [OSTI]

    Pantic, Maja

    The seven articles in this special issue focus on human computing. Most focus on two challenging issues in human computing, namely, machine analysis of human behavior in group interactions and context-sensitive modeling.

  4. Rethinking optimal control of human movements

    E-Print Network [OSTI]

    Huh, Dongsung

    2012-01-01

    movements”. In: Human muscle power, pp. 131–150. Todorov,movements”. In: Human muscle power, pp. 131–150. Todorov,movements”. In: Human muscle power, pp. 131–150. Sutton, GG

  5. Human Genome Education Program

    SciTech Connect (OSTI)

    Richard Myers; Lane Conn

    2000-05-01

    The funds from the DOE Human Genome Program, for the project period 2/1/96 through 1/31/98, have provided major support for the curriculum development and field testing efforts for two high school level instructional units: Unit 1, ''Exploring Genetic Conditions: Genes, Culture and Choices''; and Unit 2, ''DNA Snapshots: Peaking at Your DNA''. In the original proposal, they requested DOE support for the partial salary and benefits of a Field Test Coordinator position to: (1) complete the field testing and revision of two high school curriculum units, and (2) initiate the education of teachers using these units. During the project period of this two-year DOE grant, a part-time Field-Test Coordinator was hired (Ms. Geraldine Horsma) and significant progress has been made in both of the original proposal objectives. Field testing for Unit 1 has occurred in over 12 schools (local and non-local sites with diverse student populations). Field testing for Unit 2 has occurred in over 15 schools (local and non-local sites) and will continue in 12-15 schools during the 96-97 school year. For both curricula, field-test sites and site teachers were selected for their interest in genetics education and in hands-on science education. Many of the site teachers had no previous experience with HGEP or the unit under development. Both of these first-year biology curriculum units, which contain genetics, biotechnology, societal, ethical and cultural issues related to HGP, are being implemented in many local and non-local schools (SF Bay Area, Southern California, Nebraska, Hawaii, and Texas) and in programs for teachers. These units will reach over 10,000 students in the SF Bay Area and continues to receive support from local corporate and private philanthropic organizations. Although HGEP unit development is nearing completion for both units, data is still being gathered and analyzed on unit effectiveness and student learning. The final field testing result from this analysis will contribute to the final revisions of each unit during the second-year of this grant.

  6. Biomechanics of the human chorioamnion

    E-Print Network [OSTI]

    Prévost, Thibault Philippe

    2006-01-01

    The human fetal membrane, namely the chorioamnion, is the structural soft tissue retaining the amniotic fluid and the fetus during pregnancy. Its biomechanical integrity is crucial for maintaining a healthy gestation and ...

  7. Robot Manipulation in Human Environments

    E-Print Network [OSTI]

    Edsinger, Aaron

    2007-01-16

    Human environments present special challenges for robot manipulation. They are often dynamic, difficult to predict, and beyond the control of a robot engineer. Fortunately, many characteristics of these settings can be ...

  8. Robot manipulation in human environments

    E-Print Network [OSTI]

    Edsinger, Aaron Ladd, 1972-

    2007-01-01

    Human environments present special challenges for robot manipulation. They are often dynamic, difficult to predict, and beyond the control of a robot engineer. Fortunately, many characteristics of these settings can be ...

  9. Contractor Human Resource Management Programs

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1996-09-30

    The purpose of this directive is to establish Department of Energy (DOE) responsibilities and requirements for the management and oversight of contractor Human Resource Management (HR) programs. Chg 1, 5-8-98; Chg 2, 11-22-09

  10. Contractor Human Resource Management Programs

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1996-09-30

    The purpose of this directive is to establish Department of Energy (DOE) responsibilities and requirements for the management and oversight of contractor Human Resource Management (HR) programs. Chg 1, 5-8-98; Chg 2, 11-22-09.

  11. Reservations to human rights treaties 

    E-Print Network [OSTI]

    McCall-Smith, Kasey Lowe

    2012-06-26

    This thesis examines the default application of the 1969 Vienna Convention on the Law of Treaties reservation rules to reservations to human rights treaties. The contemporary practice of formulating reservations allows ...

  12. Human Factors of Reporting Systems

    E-Print Network [OSTI]

    Johnson, C.W.

    Johnson,C.W. P. Carayon (ed.), A Handbook of Human Factors and Ergonomics in Healthcare and Patient Safety, Lawrence Erlbaum, London, UK. pp 715-750 Lawrence Erlbaum Associates

  13. Ideal Observers for Detecting Human Motion: Correspondence Noise

    E-Print Network [OSTI]

    HongJing Lo; Alan Yuille

    2011-01-01

    psychophysical experiments showed that human performance waspsychophysics experiments to determine how humans performedpsychophysical experiments which are consistent with humans

  14. Structure of the SH3 domain of human osteoclast-stimulating factor at atomic resolution

    SciTech Connect (OSTI)

    Chen, Liqing Wang, Yujun; Wells, David; Toh, Diana; Harold, Hunt; Zhou, Jing; DiGiammarino, Enrico; Meehan, Edward J.

    2006-09-01

    The crystal structure of the SH3 domain of human osteoclast-stimulating factor has been determined and refined to the ultrahigh resolution of 1.07 Å. The structure at atomic resolution provides an accurate framework for structure-based design of its inhibitors. Osteoclast-stimulating factor (OSF) is an intracellular signaling protein, produced by osteoclasts themselves, that enhances osteoclast formation and bone resorption. It is thought to act via an Src-related signaling pathway and contains SH3 and ankyrin-repeat domains which are involved in protein–protein interactions. As part of a structure-based anti-bone-loss drug-design program, the atomic resolution X-ray structure of the recombinant human OSF SH3 domain (hOSF-SH3) has been determined. The domain, residues 12–72, yielded crystals that diffracted to the ultrahigh resolution of 1.07 Å. The overall structure shows a characteristic SH3 fold consisting of two perpendicular ?-sheets that form a ?-barrel. Structure-based sequence alignment reveals that the putative proline-rich peptide-binding site of hOSF-SH3 consists of (i) residues that are highly conserved in the SH3-domain family, including residues Tyr21, Phe23, Trp49, Pro62, Asn64 and Tyr65, and (ii) residues that are less conserved and/or even specific to hOSF, including Thr22, Arg26, Thr27, Glu30, Asp46, Thr47, Asn48 and Leu60, which might be key to designing specific inhibitors for hOSF to fight osteoporosis and related bone-loss diseases. There are a total of 13 well defined water molecules forming hydrogen bonds with the above residues in and around the peptide-binding pocket. Some of those water molecules might be important for drug-design approaches. The hOSF-SH3 structure at atomic resolution provides an accurate framework for structure-based design of its inhibitors.

  15. Protection of Human Research Subjects

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2009-12-09

    The order establishes Department of Energy (DOE) procedures and responsibilities for implementing the policy and requirements set forth in Title 10 Code of Federal Regulations (CFR) Part 745, Protection of Human Subjects, 45 CFR Part 46, and the Secretarial Policy Memorandum on Military or Intelligence-Related Human Subject Research, December 9, 2009. Supersedes DOE O 443.1A and DOE P 443.1A.

  16. The Politics of Human Rights in Argentina

    E-Print Network [OSTI]

    Brysk, Alison

    2013-01-01

    Valente,  Marcela.  “Argentina’s  Biggest  Human  Rights  Motor  is  Linked  to  Argentina’s  ‘Dirty  War’”.   New  fortune  of  my  heart.   Argentina's  1985  human  rights  

  17. The effects of alcohol consumption after menopause on bone regulating hormones 

    E-Print Network [OSTI]

    Blaschke, Dawn Lewis

    1999-01-01

    The goal of this project was to determine if the alcohol-associated increase in osteopenia as observed in ovariectomized rats, which simulated human females after menopause, was due to the elect of alcohol on hormones that ...

  18. TRANSLATIONAL AND CLINICAL RESEARCH Reversing Bone Loss by Directing Mesenchymal Stem

    E-Print Network [OSTI]

    Ritchie, Robert

    due to the inability to control the MSCs' commitment, growth, and differentiation into functional and is approved by Food and Drug Administration is teriparatide (recombinant human parathyroid hormone 1-34; rh

  19. Level of osteopenia and bone recovery in alcohol-fed adolescent rats 

    E-Print Network [OSTI]

    Spears, Heather Lynae

    1999-01-01

    Adolescence is a period in human growth and development that is a time of rapid and drastic change. It is also known to be an age of widespread alcohol abuse. Studies addressing the reversibility of the deleterious effects ...

  20. Flesh yours, bones mine : the making of the biomedical subject in Turkey

    E-Print Network [OSTI]

    Sanal, Aslihan

    2005-01-01

    With the emergence of biomedical technologies, human body parts from living or dead donors have become commodities in the international networks of trade. This dissertation tries to understand religious, political and ...

  1. Genomics and the human genome project: implications for psychiatry

    E-Print Network [OSTI]

    Kelsoe, J R

    2004-01-01

    300 Genomics and the Human Genome Project: implications forpast decade the Human Genome Project has made extraordinaryto each other. The Human Genome Project has approached human

  2. China and the Humanities: At the Crossroads of the Human and the Humane

    E-Print Network [OSTI]

    Tchou, W. Kang

    2013-01-01

    Genius, xx feudalism, in China, 16 fields of coeval copresence, 68 fields of copresence called configurations, 63 fifth generation directors, 117 Fifth International Confer- ence on New Directions in the Humanities, xviii film scholarship, in contem...

  3. The SACADA database for human reliability and human performance

    SciTech Connect (OSTI)

    Y. James Chang; Dennis Bley; Lawrence Criscione; Barry Kirwan; Ali Mosleh; Todd Madary; Rodney Nowell; Robert Richards; Emilie M. Roth; Scott Sieben; Antonios Zoulis

    2014-05-01

    Lack of appropriate and sufficient human performance data has been identified as a key factor affecting human reliability analysis (HRA) quality especially in the estimation of human error probability (HEP). The Scenario Authoring, Characterization, and Debriefing Application (SACADA) database was developed by the U.S. Nuclear Regulatory Commission (NRC) to address this data need. An agreement between NRC and the South Texas Project Nuclear Operating Company (STPNOC) was established to support the SACADA development with aims to make the SACADA tool suitable for implementation in the nuclear power plants' operator training program to collect operator performance information. The collected data would support the STPNOC's operator training program and be shared with the NRC for improving HRA quality. This paper discusses the SACADA data taxonomy, the theoretical foundation, the prospective data to be generated from the SACADA raw data to inform human reliability and human performance, and the considerations on the use of simulator data for HRA. Each SACADA data point consists of two information segments: context and performance results. Context is a characterization of the performance challenges to task success. The performance results are the results of performing the task. The data taxonomy uses a macrocognitive functions model for the framework. At a high level, information is classified according to the macrocognitive functions of detecting the plant abnormality, understanding the abnormality, deciding the response plan, executing the response plan, and team related aspects (i.e., communication, teamwork, and supervision). The data are expected to be useful for analyzing the relations between context, error modes and error causes in human performance.

  4. A Signal-Inducing Bone Cement for Magnetic Resonance Imaging-Guided Spinal Surgery Based on Hydroxyapatite and Polymethylmethacrylate

    SciTech Connect (OSTI)

    Wichlas, Florian, E-mail: florian.wichlas@charite.de; Seebauer, Christian J.; Schilling, Rene [University Charite, Center for Musculoskeletal Surgery (Germany); Rump, Jens [University Charite, Department of Radiology (Germany); Chopra, Sascha S. [University Charite, Center for Musculoskeletal Surgery (Germany); Walter, Thula; Teichgraeber, Ulf K. M. [University Charite, Department of Radiology (Germany); Bail, Hermann J. [University Charite, Center for Musculoskeletal Surgery (Germany)

    2012-06-15

    The aim of this study was to develop a signal-inducing bone cement for magnetic resonance imaging (MRI)-guided cementoplasty of the spine. This MRI cement would allow precise and controlled injection of cement into pathologic lesions of the bone. We mixed conventional polymethylmethacrylate bone cement (PMMA; 5 ml methylmethacrylate and 12 g polymethylmethacrylate) with hydroxyapatite (HA) bone substitute (2-4 ml) and a gadolinium-based contrast agent (CA; 0-60 {mu}l). The contrast-to-noise ratio (CNR) of different CA doses was measured in an open 1.0-Tesla scanner for fast T1W Turbo-Spin-Echo (TSE) and T1W TSE pulse sequences to determine the highest signal. We simulated MRI-guided cementoplasty in cadaveric spines. Compressive strength of the cements was tested. The highest CNR was (1) 87.3 (SD 2.9) in fast T1W TSE for cements with 4 {mu}l CA/ml HA (4 ml) and (2) 60.8 (SD 2.4) in T1W TSE for cements with 1 {mu}l CA/ml HA (4 ml). MRI-guided cementoplasty in cadaveric spine was feasible. Compressive strength decreased with increasing amounts of HA from 46.7 MPa (2 ml HA) to 28.0 MPa (4 ml HA). An MRI-compatible cement based on PMMA, HA, and CA is feasible and clearly visible on MRI images. MRI-guided spinal cementoplasty using this cement would permit direct visualization of the cement, the pathologic process, and the anatomical surroundings.

  5. ATHENA, the Desktop Human "Body"

    ScienceCinema (OSTI)

    Iyer, Rashi; Harris, Jennifer

    2015-01-05

    Creating surrogate human organs, coupled with insights from highly sensitive mass spectrometry technologies, a new project is on the brink of revolutionizing the way we screen new drugs and toxic agents. ATHENA, the Advanced Tissue-engineered Human Ectypal Network Analyzer project team, is developing four human organ constructs - liver, heart, lung and kidney - that are based on a significantly miniaturized platform. Each organ component will be about the size of a smartphone screen, and the whole ATHENA "body" of interconnected organs would fit neatly on a desk. "By developing this 'homo minutus,' we are stepping beyond the need for animal or Petri dish testing: There are huge benefits in developing drug and toxicity analysis systems that can mimic the response of actual human organs," said Rashi Iyer, a senior scientist at Los Alamos National Laboratory, the lead laboratory on the five-year, $19 million multi-institutional effort. The project is supported by the Defense Threat Reduction Agency (DTRA). Some 40 percent of pharmaceuticals fail their clinical trials, Iyer noted, and there are thousands of chemicals whose effects on humans are simply unknown. Providing a realistic, cost-effective and rapid screening system such as ATHENA with high-throughput capabilities could provide major benefits to the medical field, screening more accurately and offering a greater chance of clinical trial success.

  6. ATHENA, the Desktop Human "Body"

    SciTech Connect (OSTI)

    Iyer, Rashi; Harris, Jennifer

    2014-09-29

    Creating surrogate human organs, coupled with insights from highly sensitive mass spectrometry technologies, a new project is on the brink of revolutionizing the way we screen new drugs and toxic agents. ATHENA, the Advanced Tissue-engineered Human Ectypal Network Analyzer project team, is developing four human organ constructs - liver, heart, lung and kidney - that are based on a significantly miniaturized platform. Each organ component will be about the size of a smartphone screen, and the whole ATHENA "body" of interconnected organs would fit neatly on a desk. "By developing this 'homo minutus,' we are stepping beyond the need for animal or Petri dish testing: There are huge benefits in developing drug and toxicity analysis systems that can mimic the response of actual human organs," said Rashi Iyer, a senior scientist at Los Alamos National Laboratory, the lead laboratory on the five-year, $19 million multi-institutional effort. The project is supported by the Defense Threat Reduction Agency (DTRA). Some 40 percent of pharmaceuticals fail their clinical trials, Iyer noted, and there are thousands of chemicals whose effects on humans are simply unknown. Providing a realistic, cost-effective and rapid screening system such as ATHENA with high-throughput capabilities could provide major benefits to the medical field, screening more accurately and offering a greater chance of clinical trial success.

  7. Division of Human Resources EMERGENCY CLOSING INFORMATION

    E-Print Network [OSTI]

    Meyers, Steven D.

    Division of Human Resources EMERGENCY CLOSING INFORMATION Human Resources / Attendance and Leave of Human Resources EMERGENCY CLOSING INFORMATION Human Resources / Attendance and Leave Emergency Closing Information Form questions: (813) 974-5717 Rev. 04/2010 Disruption of Power or Water

  8. Low-Income Weatherization: The Human Dimension

    Broader source: Energy.gov [DOE]

    This presentation focuses on how the human dimension saves energy within low-income weatherization programs.

  9. Shotgun coverage of human genome computing

    E-Print Network [OSTI]

    Eddy, Sean

    take about 1.5 million pages. The Human Genome Project would not be possible if our revolution were-author collection of chapters on the various uses of computing in the Human Genome Project. Peculiarly absent fromShotgun coverage of human genome computing Human Genome Computing, Second Edition edited by Martin

  10. Doug Brutlag 2015 Sequencing the Human Genome

    E-Print Network [OSTI]

    Brutlag, Doug

    Project: Should we do it? · Service, R. F. (2001). The human genome: Objection #1: big biology is bad://www.elec-intro.com/m13-cloning #12;© Doug Brutlag 2015 Public Human Genome Project Strategy Published in Nature 15 The Human Genome Project: How should we do it? · Weber, J. L., & Myers, E. W. (1997). Human whole-genome

  11. Hominid/Human Evolution Geology 331

    E-Print Network [OSTI]

    Kammer, Thomas

    baby chimps more than adult chimps. Humans are said to be paedomorphic. #12;Neoteny in Human Evolution. Humans resemble baby apes more than adult apes. Humans are said to be paedomorphic. Chimp Gorilla #12 fossils #12;A Hominid Jawbone in Ethiopia #12;Sahelanthropus tchadensis, 6.5 MY old #12;Sahelanthropus

  12. Percentage depth dose distributions in inhomogeneous phantoms with lung and bone equivalent media for small fields of CyberKnife

    E-Print Network [OSTI]

    Lee, Chung Il; Yoon, Sei-Chul; Suh, Tae Suk; Hong, Seung-Woo; Min, Kyung Joo; Lee, Sang Deok; Chung, Su Mi; Jung, Jae-Yong

    2014-01-01

    The percentage depth dose distributions in inhomogeneous phantoms with lung and bone equivalent media are studied. For lung equivalent media a Balsa wood is used, and for a soft bone equivalent media a compound material with epoxy resin, hardener and calcium carbonate is used. Polystyrene slabs put together with these materials are used as an inhomogeneous phantom. Dose measurements are performed with Gafchromic EBT film by using photon beams from 6MV CyberKnife at the Seoul Uridul Hospital. The cone sizes of the photon beams are varied from 5, 10 to 30 mm. As a simulation tool GEANT4 Monte Carlo code v9.4.p02 is used. When the Balsa wood is inserted in the phantom, the dose measured with EBT film is found to be significantly different from the dose without the EBT film in and beyond the Balsa wood region, particularly for small field sizes. On the other hand, when the soft bone equivalent material is inserted in the phantom, discrepancy between the dose measured with EBT film and the dose without EBT film ca...

  13. FDG-PET/CT Imaging Predicts Histopathologic Treatment Responses after Neoadjuvant Therapy in Adult Primary Bone Sarcomas

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Benz, Matthias R.; Czernin, Johannes; Tap, William D.; Eckardt, Jeffrey J.; Seeger, Leanne L.; Allen-Auerbach, Martin S.; Dry, Sarah M.; Phelps, Michael E.; Weber, Wolfgang A.; Eilber, Fritz C.

    2010-01-01

    Purpose. The aim of this study was to prospectively evaluate whether FDG-PET allows an accurate assessment of histopathologic response to neoadjuvant treatment in adult patients with primary bone sarcomas.Methods. Twelve consecutive patients with resectable, primary high grade bone sarcomas were enrolled prospectively. FDG-PET/CT imaging was performed prior to the initiation and after completion of neoadjuvant treatment. Imaging findings were correlated with histopathologic response.Results. Histopathologic responders showed significantly more pronounced decreases in tumor FDG-SUVmax from baseline to late follow up than non-responders (64±19% versus29±30%, resp.;P=.03). Using a 60% decrease in tumor FDG-uptake as a threshold for metabolic response correctly classified 3more »of 4 histopathologic responders and 7 of 8 histopathologic non-responders as metabolic responders and non-responders, respectively (sensitivity, 75%; specificity, 88%).Conclusion. These results suggest that changes in FDG-SUVmax at the end of neoadjuvant treatment can identify histopathologic responders and non-responders in adult primary bone sarcoma patients.« less

  14. WILD PIG ATTACKS ON HUMANS

    SciTech Connect (OSTI)

    Mayer, J.

    2013-04-12

    Attacks on humans by wild pigs (Sus scrofa) have been documented since ancient times. However, studies characterizing these incidents are lacking. In an effort to better understand this phenomenon, information was collected from 412 wild pig attacks on humans. Similar to studies of large predator attacks on humans, data came from a variety of sources. The various attacks compiled occurred in seven zoogeographic realms. Most attacks occurred within the species native range, and specifically in rural areas. The occurrence was highest during the winter months and daylight hours. Most happened under non-hunting circumstances and appeared to be unprovoked. Wounded animals were the chief cause of these attacks in hunting situations. The animals involved were typically solitary, male and large in size. The fate of the wild pigs involved in these attacks varied depending upon the circumstances, however, most escaped uninjured. Most human victims were adult males traveling on foot and alone. The most frequent outcome for these victims was physical contact/mauling. The severity of resulting injuries ranged from minor to fatal. Most of the mauled victims had injuries to only one part of their bodies, with legs/feet being the most frequent body part injured. Injuries were primarily in the form of lacerations and punctures. Fatalities were typically due to blood loss. In some cases, serious infections or toxemia resulted from the injuries. Other species (i.e., pets and livestock) were also accompanying some of the humans during these attacks. The fates of these animals varied from escaping uninjured to being killed. Frequency data on both non-hunting and hunting incidents of wild pig attacks on humans at the Savannah River Site, South Carolina, showed quantitatively that such incidents are rare.

  15. Intelligent Escort Robot Moving together with Human Methods for Human Position Recognition

    E-Print Network [OSTI]

    Ohya, Akihisa

    : Light-emitting device used in the experiments. robot is able to know the distance to the human thanksIntelligent Escort Robot Moving together with Human ­ Methods for Human Position Recognition in everyday life by interacting with humans. In order to escort a human, the robot needs to know the position

  16. Identifying the Addressee in Human-Human-Robot Interactions based on Head Pose and Speech

    E-Print Network [OSTI]

    Schultz, Tanja

    Identifying the Addressee in Human-Human-Robot Interactions based on Head Pose and Speech Michael In this work we investigate the power of acoustic and visual cues, and their combination, to identify the addressee in a human-human-robot interaction. Based on eighteen audio- visual recordings of two human beings

  17. Human MutationSPECIAL ARTICLE Planning the Human Variome Project: The Spain Report

    E-Print Network [OSTI]

    : The remarkable progress in characterizing the human genome sequence, exemplified by the Human Genome ProjectHuman MutationSPECIAL ARTICLE Planning the Human Variome Project: The Spain Reportà Jim Kaput,1yz-Sook Yoo,93 on behalf of contributors to the Human Variome Project Planning Meeting 1 Division

  18. Somatic cell genotoxicity at the glycophorin A locus in humans

    SciTech Connect (OSTI)

    Jensen, R.H.; Grant, S.G.; Langlois, R.G.; Bigbee, W.L.

    1990-12-28

    We have developed an assay for detecting variant erythrocytes that occur as a result of in vivo allele loss at the glycophorin A (GPA) locus on chromosome 4 in humans. This gene codes for an erythroid- specific cell surface glycoprotein, and with our assay we are able to detect rare variant erythrocytes that have lost expression of one of the two GPA alleles. Two distinctly different variant cell types are detected with this assay. One variant cell type (called N{O}) is hemizygous. Our assay also detects homozygous variant erythrocytes that have lost expression of the GPA(M) allele and express the GPA(N) allele at twice the heterozygous level. The results of this assay are an enumeration of the frequency of N{O} and NN variant cell types for each individual analyzed. These variant cell frequencies provide a measure of the amount of somatic cell genotoxicity that has occurred at the GPA locus. Such genotoxicity could be the result of (1) reactions of toxic chemicals to which the individual has been exposed, or (2) high energy radiation effects on erythroid precursor cells, or (3) errors in DNA replication or repair in these cells of the bone marrow. Thus, the GPA-based variant cell frequency can serve as a biodosimeter that indicates the amount of genotoxic exposure each individual has received. Because two very different kinds of variant cells are enumerated, different kinds of genotoxicity should be distinguishable. Results of the GPA somatic genotoxicity assay may also provide valuable information for cancer-risk estimation on each individual. 16 refs.

  19. Human factors in software development

    SciTech Connect (OSTI)

    Curtis, B.

    1986-01-01

    This book presents an overview of ergonomics/human factors in software development, recent research, and classic papers. Articles are drawn from the following areas of psychological research on programming: cognitive ergonomics, cognitive psychology, and psycholinguistics. Topics examined include: theoretical models of how programmers solve technical problems, the characteristics of programming languages, specification formats in behavioral research and psychological aspects of fault diagnosis.

  20. Human retroviruses and AIDS 1994

    SciTech Connect (OSTI)

    Myers, G.; Korber, B.; Wain-Hobson, S.; Jeang, Kuan-Teh; Henderson, L.E.; Pavlakis, G.N.

    1995-01-01

    This compendium, including accompanying floppy diskettes, is the result of an effort to compile and rapidly publish all relevant molecular data concerning the human immunodeficiency viruses (HIV) and related retroviruses. The scope of the compendium and database is best summarized by the five parts it comprises: (I) Nucleic Acid Alignments and Sequences; (II) Amino Acid Alignments; (III) Analysis; (IV) Related Sequences; (V) Database communications.

  1. Human Resources, Safety & Risk Management

    E-Print Network [OSTI]

    1 Human Resources, Safety & Risk Management 1600 Holloway Avenue, ADM 252 San Francisco, California OF RISK AND AGREEMENT TO PAY CLAIMS Activity: San Francisco State University Campus Recreation Department participating in this Activity. I am aware of the risks associated with traveling to/from and participating

  2. Analysis of Human Genetic Linkage

    SciTech Connect (OSTI)

    Boehnke, M.

    1991-01-01

    Linkage analysis continues in its golden age. The convergence of several factors - advances in molecular biology, advances in statistical models and algorithms, and advances in computing technology - have made possible remarkable successes in the mapping of human genetic diseases and in the construction of human genetic maps. The goals of mapping all the most important simple Mendelian disorders and constructing fine-structure genetic maps for each of the human chromosomes soon will be reached, and linkage methods promise to help us understand the etiologies of many common and complex familial diseases. With the continuing rapid advance of the field, the appearance of the revised edition of Dr. Ott's book is particularly welcome. As with the first edition, the goal of the revised edition is to provide a concise, easy-to-read introduction to human linkage analysis. The revised edition includes chapters on basic genetics and cytogenetics, genes and genetic polymorphisms, aspects of statistical inference, methods of linkage analysis, the informativeness of family data, multipoint linkage analysis, penetrance, numerical and computerized methods, the variability of the recombination fraction, inconsistencies, and linkage analysis with disease loci. The results is not an encyclopedia providing everything one could ever want to know about linkage analysis but, rather, a guide to the important methods, topics, and problems of linkage analysis today. Overall, the book achieves an excellent compromise between presenting important conclusions and working out the details.

  3. POSITION DESCRIPTIONS GUIDELINE HUMAN RESOURCES

    E-Print Network [OSTI]

    Su, Xiao

    and activities related to successful human resources operations such as: recruitment and selection, job may also help you develop recruiting materials, and ensure consistency and equity among positions of Labor (O*Net), or the Bureau of Labor Statistics (Occupational Outlook Handbook) may be excellent

  4. SALARY ADVANCE REQUEST HUMAN RESOURCES

    E-Print Network [OSTI]

    Su, Xiao

    SALARY ADVANCE REQUEST HUMAN RESOURCES Employee Support Services | One Washington Square | San José, CA 95192-0046 | 408-924-2250 408-924-1701 (fax) Instructions: A request for a salary advance should: Department: Division/College: Campus Phone #: Employee Status: (check one) Faculty Staff REASON FOR SALARY

  5. EMERGENCY HIRE GUIDELINES HUMAN RESOURCES

    E-Print Network [OSTI]

    Su, Xiao

    EMERGENCY HIRE GUIDELINES HUMAN RESOURCES Workforce Planning · One Washington Square · San José, Ca 95192-0046 · Phone 408-924-2250 · Fax 408-924-1784 SUBJECT: EMERGENCY HIRES RESPONSIBLE UNIT & CONTACT: Workforce Planning; Sr. Workforce Planning Analyst DATE: May 2, 2008 I. PURPOSE / DESCRIPTION The Emergency

  6. A Literary Human Exinction Scenario

    SciTech Connect (OSTI)

    Tonn, Bruce Edward

    2009-11-01

    Mary Wollstonecraft Shelly's (MWS) novel, The Last Man, published in 1826, is an epic narrative about the destruction of the human race. This paper provides a synopsis of this book and assesses its relationships to contemporary future studies. The paper also delves into the history of apocalyptic writing and thinking, using this book an entry point to past literature.

  7. COLLEGE OF ENGINEERING Human Health

    E-Print Network [OSTI]

    COLLEGE OF ENGINEERING Human Health #12;Welcome to our Health issue ­ Please take the time to read the exciting stories about how our faculty, students, and alumni are engineering solutions to health care to tackle large worldwide health problems. A few years ago, the College of Engineering made a strategic

  8. Transcriptional regulation of human RANK ligand gene expression by E2F1

    SciTech Connect (OSTI)

    Hu Yan [Department of Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263 (United States); Sun Meng [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Graduate Program of Pharmacology and Toxicology, University of California at Davis, Sacramento, CA 95817 (United States); Nadiminty, Nagalakshmi; Lou Wei [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Pinder, Elaine [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Graduate Program of Pharmacology and Toxicology, University of California at Davis, Sacramento, CA 95817 (United States); Gao, Allen C. [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Graduate Program of Pharmacology and Toxicology, University of California at Davis, Sacramento, CA 95817 (United States)], E-mail: acgao@ucdavis.edu

    2008-06-06

    Receptor activator of nuclear factor kappa B ligand (RANKL) is a critical osteoclastogenic factor involved in the regulation of bone resorption, immune function, the development of mammary gland and cardiovascular system. To understand the transcriptional regulation of RANKL, we amplified and characterized a 1890 bp 5'-flanking sequence of human RANKL gene (-1782 bp to +108 bp relative to the transcription start site). Using a series of deletion mutations of the 1890 bp RANKL promoter, we identified a 72 bp region (-172 to -100 bp) mediating RANKL basal transcriptional activity. Sequence analysis revealed a putative E2F binding site within this 72 bp region in the human RANKL promoter. Overexpression of E2F1 increased RANKL promoter activity, while down-regulation of E2F1 expression by small interfering RNA decreased RANKL promoter activity. RT-PCR and enzyme linked immunosorbent assays (ELISA) further demonstrated that E2F1 induced the expression of RANKL. Electrophoretic gel mobility shift assays (EMSA) and antibody competition assays confirmed that E2F1 proteins bind to the consensus E2F binding site in the RANKL promoter. Mutation of the E2F consensus binding site in the RANKL promoter profoundly reduced the basal promoter activity and abolished the transcriptional modulation of RANKL by E2F1. These results suggest that E2F1 plays an important role in regulating RANKL transcription through binding to the E2F consensus binding site.

  9. Mechanosensitivity of human osteosarcoma cells and phospholipase C {beta}2 expression

    SciTech Connect (OSTI)

    Hoberg, M. [Department of Orthopaedics, University of Tuebingen (Germany)]. E-mail: Maik.Hoberg@med.uni-tuebingen.de; Gratz, H.-H. [Experimental Orthopaedics and Biomechanics, Phillips-University of Marburg (Germany); Noll, M. [Experimental Orthopaedics and Biomechanics, Phillips-University of Marburg (Germany); Jones, D.B. [Experimental Orthopaedics and Biomechanics, Phillips-University of Marburg (Germany)

    2005-07-22

    Bone adapts to mechanical load by osteosynthesis, suggesting that osteoblasts might respond to mechanical stimuli. We therefore investigated cell proliferation and phospholipase C (PLC) expression in osteoblasts. One Hertz uniaxial stretching at 4000 {mu}strains significantly increased the proliferation rates of human osteoblast-like osteosarcoma cell line MG-63 and primary human osteoblasts. However, U-2/OS, SaOS-2, OST, and MNNG/HOS cells showed no significant changes in proliferation rate. We investigated the expression pattern of different isoforms of PLC in these cell lines. We were able to detect PLC {beta}1, {beta}3, {gamma}1, {gamma}2, and {delta}1 in all cells, but PLC {beta}2 was only detectable in the mechanosensitive cells. We therefore investigated the possible role of PLC {beta}2 in mechanotransduction. Inducible antisense expression for 24 h inhibited the translation of PLC {beta}1 in U-2/OS cells by 35% and PLC {beta}2 in MG-63 by 29%. Fluid shear flow experiments with MG-63 lacking PLC {beta}2 revealed a significantly higher level of cells losing attachment to coverslips and a significantly lower number of cells increasing intracellular free calcium.

  10. Patterns of Practice in Palliative Radiotherapy for Painful Bone Metastases: A Survey in Japan

    SciTech Connect (OSTI)

    Nakamura, Naoki; Shikama, Naoto; Wada, Hitoshi; Harada, Hideyuki; Nozaki, Miwako; Nagakura, Hisayasu; Tago, Masao; Oguchi, Masahiko; Uchida, Nobue

    2012-05-01

    Purpose: To determine the current patterns of practice in Japan and to investigate factors that may make clinicians reluctant to use single-fraction radiotherapy (SF-RT). Methods and Materials: Members of the Japanese Radiation Oncology Study Group (JROSG) completed an Internet-based survey and described the radiotherapy dose fractionation they would recommend for four hypothetical cases describing patients with painful bone metastasis (BM). Case 1 described a patient with an uncomplicated painful BM in a non-weight-bearing site from non-small-cell lung cancer. Case 2 investigated whether management for a case of uncomplicated spinal BM would be different from that in Case 1. Case 3 was identical with Case 2 except for the presence of neuropathic pain. Case 4 investigated the prescription for an uncomplicated painful BM secondary to oligometastatic breast cancer. Radiation oncologists who recommended multifraction radiotherapy (MF-RT) for Case 2 were asked to explain why they considered MF-RT superior to SF-RT. Results: A total of 52 radiation oncologists from 50 institutions (36% of JROSG institutions) responded. In all four cases, the most commonly prescribed regimen was 30 Gy in 10 fractions. SF-RT was recommended by 13% of respondents for Case 1, 6% for Case 2, 0% for Case 3, and 2% for Case 4. For Case 4, 29% of respondents prescribed a high-dose MF-RT regimen (e.g., 50 Gy in 25 fractions). The following factors were most often cited as reasons for preferring MF-RT: 'time until first increase in pain' (85%), 'incidence of spinal cord compression' (50%), and 'incidence of pathologic fractures' (29%). Conclusions: Japanese radiation oncologists prefer a schedule of 30 Gy in 10 fractions and are less likely to recommend SF-RT. Most Japanese radiation oncologists regard MF-RT as superior to SF-RT, based primarily on the time until first increase in pain.

  11. Spatio-temporal Neuroimaging of Visual Processing of Human and Robot Actions in Humans

    E-Print Network [OSTI]

    Urgen, Burcu Aysen

    2015-01-01

    H. (2006). Human-inspired robots. IEEE Intelligent Sys. 21,K. (2007). Socially intelligent robots: dimensionsof human-robot interaction. Philos. Trans. R. Soc. Lond. B

  12. Human Fertilisation and Embryology Bill A briefing for MPs on the need for research involving human and human admixed embryos.

    E-Print Network [OSTI]

    Rambaut, Andrew

    cells for research. The Bill allows research, under license, on four types of HAEs, which contain bothHuman Fertilisation and Embryology Bill A briefing for MPs on the need for research involving human and human admixed embryos. Prepared by the Academy of Medical Sciences, the Medical Research Council

  13. Multipotent human stromal cells improve cardiac function after myocardial infarction in mice without long-term engraftment

    SciTech Connect (OSTI)

    Iso, Yoshitaka [Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States); Cardiovascular Research Institute, Department of Medicine, University of Vermont, 208 South Park Drive, Colchester, VT 05446 (United States); Spees, Jeffrey L. [Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States); Cardiovascular Research Institute, Department of Medicine, University of Vermont, 208 South Park Drive, Colchester, VT 05446 (United States); E-mail: Jeffrey.Spees@uvm.edu; Serrano, Claudia [Section of Cardiology, Department of Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States); Bakondi, Benjamin [Cardiovascular Research Institute, Department of Medicine, University of Vermont, 208 South Park Drive, Colchester, VT 05446 (United States); Pochampally, Radhika [Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States); Song, Yao-Hua [Section of Cardiology, Department of Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States); Sobel, Burton E. [Cardiovascular Research Institute, Department of Medicine, University of Vermont, 208 South Park Drive, Colchester, VT 05446 (United States); Delafontaine, Patrick [Section of Cardiology, Department of Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States); Prockop, Darwin J. [Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112 (United States)]. E-mail: dprocko@tulane.edu

    2007-03-16

    The aim of this study was to determine whether intravenously administered multipotent stromal cells from human bone marrow (hMSCs) can improve cardiac function after myocardial infarction (MI) without long-term engraftment and therefore whether transitory paracrine effects or secreted factors are responsible for the benefit conferred. hMSCs were injected systemically into immunodeficient mice with acute MI. Cardiac function and fibrosis after MI in the hMSC-treated group were significantly improved compared with controls. However, despite the cardiac improvement, there was no evident hMSC engraftment in the heart 3 weeks after MI. Microarray assays and ELISAs demonstrated that multiple protective factors were expressed and secreted from the hMSCs in culture. Factors secreted by hMSCs prevented cell death of cultured cardiomyocytes and endothelial cells under conditions that mimicked tissue ischemia. The favorable effects of hMSCs appear to reflect the impact of secreted factors rather than engraftment, differentiation, or cell fusion.

  14. Human-Centered Sustainable Product Design

    E-Print Network [OSTI]

    Agogino, Alice M.

    Inquiry ­! Green Building Design !! Week 2 ­! Brainstorming and Creative Design ­! Life Cycle Analysis / Sustainability Needs Human- centered design Uneconomical green design Unadopted green systems Sustainable systemsHuman-Centered Sustainable Product Design !!Module Overview !!Need Finding Methods 1 ME Module

  15. Human Dimensions of Wildlife Research Norman Dandy

    E-Print Network [OSTI]

    Human Dimensions of Wildlife Research Norman Dandy Social & Economic Research Group #12;Wildlife) · Human-dimensions of species management (HDSM) Research Projects #12;Collaborative Frameworks for Land of woodland landscapes ­ discussion groups, · Choice experiments, · Fellowships / Placements, · Newsletters

  16. Bioinformatics for the human microbiome project

    E-Print Network [OSTI]

    Gevers, Dirk

    Microbes inhabit virtually all sites of the human body, yet we know very little about the role they play in our health. In recent years, there has been increasing interest in studying human-associated microbial communities, ...

  17. Epidemiology, classification and evolution of human rhinoviruses 

    E-Print Network [OSTI]

    McIntyre, Chloe Leanne

    2013-07-06

    Human rhinoviruses (HRV) are extremely common human respiratory pathogens, most commonly associated with mild upper respiratory tract infections. The three known species of HRV (HRV-A, -B and –C) are members of the family ...

  18. Propeller based human powered swimming device

    E-Print Network [OSTI]

    Bunker, Kristine (Kristine Alina)

    2014-01-01

    Currently the only human powered swimming device widely sold on the market are swim flippers. However, flippers are not efficient for the human body, and there is a potential to increase the speed while swimming with a ...

  19. Original Paper Human sensorimotor learning for

    E-Print Network [OSTI]

    Babic, Jan

    . The concept is to consider the target robot platform as a tool to be controlled intuitively by a human to drive a car. After human learning, the skilled control of the robot is used to build an autonomous

  20. NIST Update NIST Human Identity Project Team

    E-Print Network [OSTI]

    NIST Update NIST Human Identity Project Team National Institute of Standards and Technology Standard Set (ESS) STRs using the CEPH human genome diversity panel, Forensic Sci. Int. Genet. (2010), doi