Sample records for hondu ras jamaica

  1. Jamaica Bay New York's Conflicted Backyard

    E-Print Network [OSTI]

    Columbia University

    Jamaica Bay ­ New York's Conflicted Backyard Kevin Olsen Drew Symposium October 2008 #12;Why Study Jamaica Bay's History? Jamaica Bay has served many uses for the people of New York. At one time or another shoals, mudflats, sand bars, open water (littoral zone), intertidal zones (low and high marshes

  2. Jamaica-Caribbean Community (CARICOM) Sustainable Energy Roadmap...

    Open Energy Info (EERE)

    Jamaica-Caribbean Community (CARICOM) Sustainable Energy Roadmap and Strategy Jump to: navigation, search Name Jamaica-Caribbean Community (CARICOM) Sustainable Energy Roadmap and...

  3. Intersection : public place in a new Jamaica

    E-Print Network [OSTI]

    Young, Kevin Anthony

    1995-01-01T23:59:59.000Z

    Jamaica, a microcosm of the Caribbean and the developing world, is heir to an ambivalent legacy. While she benefits from a unique cultural tradition brought in part through colonialism, she suffers from the nihilistic ...

  4. The probate inventories of Port Royal, Jamaica

    E-Print Network [OSTI]

    Thornton, Diana Vida

    1992-01-01T23:59:59.000Z

    THE PROBATE INVENTORIES OF PORT ROYAL, JAMAICA A Thesis by DIANA VIDA THORNTON Submitted to the Office of Graduate Studies of Texas A8cM University in partial fulfillment of the requirements for the degree of MASTER OF ARTS December 1992... Major Subject: Anthropology THE PROBATE INVENTORIES OF PORT ROYAL, JAMAICA A Thesis by DIANA VIDA THORNTON Approved as to style and content by: D. L. Hamilton, Anthropology (Chair of Committee) Kevrn Crisman, Anthropology (Member) James M...

  5. RAS Update February 2012 Page 1 RAS Update, February 2012

    E-Print Network [OSTI]

    Heller, Eric

    , March 13th International research, education, and development projects constitute a growing market information on the following: Welcome to Our New Colleagues Upcoming Research Administration Events RAS Case Studies Workshop: Spending Sponsored Funds ­ Wednesday, March 7th RAS Workshop: Equipment

  6. Jamaica: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica: Energy Resources

  7. Analysis of the hollowware pewter from Port Royal, Jamaica

    E-Print Network [OSTI]

    Winslow, Debra Lynn

    2000-01-01T23:59:59.000Z

    Over the past forty years an enormous collection of pewter artifacts has been recovered from the various archaeological excavations at Port Royal, Jamaica, both on land and underwater (Link 1960; Marx 1971; Priddy 1975; Hamilton 1984, 1985, 1987...

  8. Digital Signaling and Hysteresis Characterize Ras Activation in Lymphoid Cells

    E-Print Network [OSTI]

    Das, Jayajit

    Activation of Ras proteins underlies functional decisions in diverse cell types. Two molecules, RasGRP and SOS, catalyze Ras activation in lymphocytes. Binding of active Ras to SOS' allosteric pocket markedly increases ...

  9. Matching renewal energy sources to rural development needs : a prototype design for a rural community development center for Jamaica, W.I.

    E-Print Network [OSTI]

    Jackson, Michael Onaje

    1982-01-01T23:59:59.000Z

    The opportunities for utilizing Jamaica/s rich supply of renewable energy resources as a base for stead, environmentally sound rural development is tremendous. This thesis explores as way of tapping this potential. Jamaica's ...

  10. Effects of the Alvenus oil spill on Jamaica beach macrofauna 

    E-Print Network [OSTI]

    Sweet, Merrill Henry

    1987-01-01T23:59:59.000Z

    EFFECTS OF THE ALVENUS OIL SPILL ON JAMAICA BEACH MACROFAUNA A Thesis by MERRILL HENRY SWEET Submitted to the Graduate College of Texas A&M U n i v e r s i t y in p a r t i a l f u l f i l l m e n t of the requirement f o r the degree....\\ Bright^ (Member) Timothy C. H a l l (Head of Department) August 1987 i i i ABSTRACT E f f e c t s of the Alvenus Oil S p i l l on Jamaica Beach Macrofauna. (August 1987) M e r r i l l Henry Sweet, B.S., Texas A&M U n i v e r s i t y Chair...

  11. Effects of the Alvenus oil spill on Jamaica beach macrofauna

    E-Print Network [OSTI]

    Sweet, Merrill Henry

    1987-01-01T23:59:59.000Z

    e c t e d at Jamaica beach, 1983 41 9. Number of i n d i v i d u a l s , percent composition of t o t a l number of i n d i v i d u a l s , biomass, and percent composition of t o t a l biomass f o r species c o l l e c t e d at Jamaica beach... reductions to < 5% of pre- s p i l l biomasses at the most impacted s t a t i o n . Their t o l e r a n c e f o r o i l often enables polychaetes to be some of the most abundant animals l e f t i n the community (Lewbel 1985). In 9 f a c t , polychaetes...

  12. RAs

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis andBHoneywell9/%2A en International Nuclear

  13. RAS FRIDAYS A Monthly Networking Meeting for FAS Research Administrators

    E-Print Network [OSTI]

    RAS FRIDAYS A Monthly Networking Meeting for FAS Research Administrators RAS FRIDAY MEETING AGENDA to Research Administration Services (RAS) Friday Patrick Fitzgerald Associate Dean for Research Administration FAS Research Administration Services (RAS) 10:10 Office for Technology Development Updates Julie

  14. Optimal Siting of Regional Fecal Sludge Treatment Facilities: St. Elizabeth, Jamaica

    E-Print Network [OSTI]

    Vogel, Richard M.

    Optimal Siting of Regional Fecal Sludge Treatment Facilities: St. Elizabeth, Jamaica Ana Martha- ated with their mismanagement and deterioration. Historically, fecal sludge management has been-9496 2008 134:1 55 CE Database subject headings: Sludge; System analysis; Waste stabilization ponds

  15. Estrogen: A master regulator of bioenergetic systems in the brain and Jamaica R. Rettberg a

    E-Print Network [OSTI]

    Brinton, Roberta Diaz

    Review Estrogen: A master regulator of bioenergetic systems in the brain and body Jamaica R is coincident with decline in brain bioenergetics and shift towards a metabolically compromised phenotype. Compensatory bioenergetic adaptations, or lack thereof, to estrogen loss could determine risk of late

  16. The readers point vessel: hull analysis of an eighteenth century merchant sloop excavated in St. Ann's Bay, Jamaica

    E-Print Network [OSTI]

    Cook, Gregory D.

    1997-01-01T23:59:59.000Z

    's Bay, Jamaica in 1994. Excavators removed overburden and the ballast pile, recovering over 600 artifacts associated with the vessel-After exposing well-preserved hull remains, divers recorded the ship's structure. The vessel is preserved from the base...

  17. Second Line of Defense Megaports Initiative Operational Testing and Evaluation Plan - Kingston Container Terminal, Port of Kingston, Jamaica

    SciTech Connect (OSTI)

    Deforest, Thomas J.; VanDyke, Damon S.

    2012-03-01T23:59:59.000Z

    Operational Testing and Evaluation Plan - Kingston Container Terminal, Port of Kingston, Jamaica was written for the Second Line of Defense Megaports Initiative. The purpose of the Operational Testing and Evaluation (OT&E) phase of the project is to prepare for turnover of the Megaports system supplied by U.S. Department of Energy/National Nuclear Security Administration (DOE/NNSA) located at the Kingston Container Terminal (KCT) of the Port of Kingston, Jamaica to the Government of Jamaica (GOJ). Activities conducted during the OT&E phase must demonstrate that the Megaports system can be operated effectively in real time by Jamaica Customs and KCT personnel to the satisfaction of the DOE/NNSA. These activities will also determine if the Megaports system, as installed and accepted, is performing according to the Megaports Program objectives such that the system is capable of executing the mission of the Second Line of Defense Megaports Initiative. The OT&E phase of the project also provides an opportunity to consider potential improvements to the system and to take remedial action if performance deficiencies are identified during the course of evaluation. Changes to the system should be considered under an appropriate change-control process. DOE/NNSA will determine that OT&E is complete by examining whether the Megaports system is performing as intended and that the GOJ is fully capable of operating the system independently without continued onsite support from the U.S. team.

  18. Review of Jamaica Public Service Company, Ltd. least-cost expansion plan.

    SciTech Connect (OSTI)

    Koritarov, V.; Buehring, W.; Cirillo, R.; Decision and Information Sciences

    2008-02-28T23:59:59.000Z

    Argonne National Laboratory has been asked to review the least-cost expansion plan (LCEP) of the Jamaica Public Service Company, Ltd. (JPSCo). The material that has been initially provided to Argonne included: (1) An electronic copy of the data and results from JPSCo's running the WASP electric system expansion planning model, (2) Approximately 20 pages of a document 'JPSCo Generation Expansion Plan', marked 'DRAFT 002', date unknown, and (3) The report 'JPSCo Least Cost Generation Expansion Plans, (1999-2009)', January 1999. It was noticed that the 20 pages from the 'DRAFT 002' document were different from the January 1999 report. An explanation was provided to Argonne that the excerpt was from an earlier draft and that the review should focus on the January 1999 report. Further, the electronic copy of the WASP case did not correspond to either the January 1999 report or to the 20-page excerpt. Again, the reason for these discrepancies was that the WASP case provided to Argonne was an earlier case and not the final one that was presented in the report. Based on the review of the available material, Argonne experts have prepared and submitted to the National Investment Bank of Jamaica (NIBJ) a preliminary draft report containing the initial findings, comments, questions and observations. As many of the comments and questions raised in the preliminary review needed to be discussed with the appropriate staff of JPSCo and other Jamaican experts, a 3-day mission to Jamaica was carried out by one Argonne expert (V. Koritarov) in the period July 20-23, 1999. Besides JPSCo experts, the discussions and the review of the LCEP during the mission included several experts from NIBJ, Ministry of Energy, and the Petroleum Corporation of Jamaica. Mr. Koritarov also worked with the JPSCo technical staff to reconstruct the WASP base case that was used as a basis for the January 1999 report. The first step was to verify that the results obtained after the resimulation of this case were identical to those presented in the January 1999 report. Then, in the next step, the Argonne expert and JPSCo team reviewed this case in detail and performed certain modifications and improvements of data where necessary. These modifications and data adjustments resulted in a new base case that served as a basis for further review and for the sensitivity analyses. Several sensitivity analyses were performed together with JPSCo experts and the results were discussed with the JPSCo management and other Jamaican experts at the end of the mission. Additional sensitivity analyses, as well as the cases for high and low load forecasts, were conducted by Mr. Koritarov after returning from Jamaica. The main findings of the review and issues that have been discussed with the Jamaican team can be summarized.

  19. The C-Terminus of H-Ras as a Target for the Covalent Binding of Reactive Compounds Modulating Ras-

    E-Print Network [OSTI]

    Pompeu Fabra, Universitat

    ´ficas, Madrid, Spain, 2 Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain, 3 Department of Organic Chemistry, Faculty of Sciences, University of Ma´laga, Ma´laga, Spain, 4 Microbiologi´a, Instituto de Salud Carlos III, Madrid, Spain Abstract Ras proteins are crucial players

  20. Effects of oncogenic Ras and p38 mitogen-activated protein kinase on the adhesion of normal human cells

    E-Print Network [OSTI]

    Waldman, Lynne K

    2010-01-01T23:59:59.000Z

    Activating mutations in RAS oncogenes commonly arise in human cancers. However, in experimental settings, oncogenic RAS has most often been studied at supraphysiological levels of expression. Importantly, work by others ...

  1. R-Ras Regulates Migration through an Interaction with Filamin A in Melanoma Cells

    E-Print Network [OSTI]

    Ramos, Joe W.

    R-Ras Regulates Migration through an Interaction with Filamin A in Melanoma Cells Joanna E. Gawecka and b7 tails and is associated with diverse cell processes including cell migration. Indeed, M2 melanoma melanoma cells active R-Ras co-localized with FLNa but did not co-localize with FLNa lacking repeat 3. Thus

  2. Description and analysis of flintlock pistols recovered from a seventeenth-century shipwreck on Pedro Bank, Jamaica

    E-Print Network [OSTI]

    Garigen, Lisa Lynn

    1991-01-01T23:59:59.000Z

    a definitive catalog of all the pistols recovered from the Spanish fleet ship that sunk in 1691 on Pedro Bank, Jamaica. To this end, I will present a brief history of the Spanish fleets to provide general knowledge about the Tierra Firme fleet... quarter of the 17th century and thereafter, their use spread rapidly. By the time the 1691 Tierra Firme Fleet was lost on Pedro Bank, the use of flintlock pistols was almost universal. They were being built throughout Europe and even in some parts...

  3. GTPase Catalysis by Ras and Other G-proteins: Insights from Substrate Directed SuperImposition

    E-Print Network [OSTI]

    Kosloff, Mickey

    GTPase Catalysis by Ras and Other G-proteins: Insights from Substrate Directed Super usage of similar approaches to comparison of enzyme catalytic machineries. We applied SDSI to various G catalysis; GTPase; guanine nucleotides; conformational rearrangement; structure comparison

  4. The nitric oxide-sensitive p21Ras-ERK pathway mediates S-nitrosoglutathione-induced apoptosis

    SciTech Connect (OSTI)

    Tsujita, Maristela [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil); Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo, SP (Brazil); Batista, Wagner L.; Ogata, Fernando T. [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil); Stern, Arnold [Department of Pharmacology, New York School of Medicine, New York University, New York (United States); Monteiro, Hugo P. [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil)], E-mail: hpmonte@uol.com.br; Arai, Roberto J. [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil)], E-mail: arairj@yahoo.com.br

    2008-05-16T23:59:59.000Z

    p21Ras protein plays a critical role in cellular signaling that induces either cell cycle progression or apoptosis. Nitric oxide (NO) has been consistently reported to activate p21Ras through the redox sensitive cysteine residue (118). In this study, we demonstrated that the p21Ras-ERK pathway regulates THP-1 monocyte/macrophage apoptosis induced by S-nitrosoglutathione (SNOG). This was apparent from studies in THP-1 cells expressing NO-insensitive p21Ras (p21Ras{sup C118S}) where the pro-apoptotic action of SNOG was almost abrogated. Three major MAP kinase pathways (ERK, JNK, and p38) that are downstream to p21Ras were investigated. It was observed that only the activation of ERK1/2 MAP kinases by SNOG in THP-1 cells was attributable to p21Ras. The inhibition of the ERK pathway by PD98059 markedly attenuated apoptosis in SNOG-treated THP-1 cells, but had a marginal effect on SNOG-treated THP-1 cells expressing NO-insensitive p21Ras. The inhibition of the JNK and p38 pathways by selective inhibitors had no marked effects on the percentage of apoptosis. The induction of p21Waf1 expression by SNOG was observed in THP-1 cells harboring mutant and wild-type p21Ras, however in cells expressing mutant Ras, the expression of p21Waf1 was significantly attenuated. The treatment of THP-1 cells expressing wild-type p21Ras with PD98059 resulted in significant attenuation of p21Waf1 expression. These results indicate that the redox sensitive p21Ras-ERK pathway plays a critical role in sensing and delivering the pro-apoptotic signaling mediated by SNOG.

  5. Qatargas exporting LNG from Qatar`s new Ras Laffan Port

    SciTech Connect (OSTI)

    NONE

    1997-02-24T23:59:59.000Z

    When the 135,000 cu m LNG carrier Al Zubarah departed Ras Laffan Port in December, Qatar entered a new era of commerce that will both boost the emirate`s economic development and influence energy trade around the world. The event capped more than a decade of planning, design, and construction of Ras Laffan Port--the world`s newest and largest LNG exporting facility. During the 1980s, the focus in Qatar was on exploration and development of North field, which holds the world`s largest reserves of nonassociated natural gas. In the 1990s, efforts concentrated on establishing a direct production and export link between North field, the new multi-billion-dollar Qatar Liquefied Gas Co. (Qatargas) gas liquefaction plant at Ras Laffan, and LNG export facilities at the 8.5 sq km Ras Laffan Port. Markets of the Far East will be first to be served by LNG from Ras Laffan Port. Two 25-year LNG supply contracts have been signed with buyers in Japan and South Korea, and negotiations are under way with potential customers from China, Taiwan, and Thailand. The paper describes the port, its operations, and export projects.

  6. RAS Mutations Impact TNF-Induced Apoptosis in Colon Carcinoma Cells via ERK-Modulatory Negative and Positive Feedback Circuits along with non-ERK Pathway Effects

    E-Print Network [OSTI]

    Lauffenburger, Douglas A.

    More than 40% of colon cancers have a mutation in K-RAS or N-RAS, GTPases that operate as central hubs for multiple key signaling pathways within the cell. Utilizing an isogenic panel of colon carcinoma cells with K-RAS ...

  7. RAS mutation is associated with hyperdiploidy and parental characteristics in pediatric acute lymphoblastic leukemia

    E-Print Network [OSTI]

    California at Berkeley, University of

    RAS mutation is associated with hyperdiploidy and parental characteristics in pediatric acute lymphoblastic leukemia JL Wiemels1 , Y Zhang1 , J Chang2 , S Zheng3 , C Metayer2 , L Zhang2 , MT Smith2 , X Ma4 and cytogenetic factors in a case series of children with leukemia. Diagnostic bone marrow samples from 191

  8. Paper presented at IRRDB, 2006, Vietnam. Rubber based Agroforestry Systems (RAS) as Alternatives for Rubber Monoculture

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Paper presented at IRRDB, 2006, Vietnam. Rubber based Agroforestry Systems (RAS) as Alternatives for Rubber Monoculture System. Gede Wibawa Lembaga Riset Perkebunan Indonesia Jalan Salak 1A, Bogor Eric Penot CIRAD BP 5035, 34032 Montpellier, Cedex 1, France ABSTRACT Smallholder rubber plantations

  9. Health-hazard-evaluation report HETA 87-371-2000, Technical Assistance to the Jamaican Ministry of Health, Kingston, Jamaica

    SciTech Connect (OSTI)

    Matte, T.D.; Burr, G.A.

    1989-12-01T23:59:59.000Z

    In response to a request from the Jamaican Ministry of Health, a study was made of possible health hazards existing due to the operation of a secondary lead smelter in Saint Catherine Parish, Jamaica. Emission controls at the site were upgraded in 1974. A cottage industry of clandestine backyard smelters was also in operation in the area. The survey investigated the health of residents in 58 households in the Red Pond Road community and 21 households in Ebony Vale. Soil lead levels in Red Pond exceeded 500 parts per million at 24% of the households tested. Ten paint samples taken from Red Pond homes exceeded 1% lead by weight. The geometric mean blood lead level of those tested in Red Pond was more than twice the level found in those tested in Ebony Vale. Forty four percent of the children under 6 years of age in Red Pond had blood lead levels above 25 micrograms/deciliter. Soil lead contamination was the strongest predictor of blood lead levels among the Red Pond subjects under 12 years of age. Lead smelter work was an important predictor in the older subjects. The authors conclude that the backyard smelters in combination with the secondary smelter caused a high lead poisoning risk for area residents. The authors recommend that residents stop the backyard smelting operations, and that efforts be made to reduce exposure through hazard abatement, education, and establishment of clean play areas for children.

  10. RAS Mutations Affect Tumor Necrosis Factor–Induced Apoptosis in Colon Carcinoma Cells via ERK-Modulatory Negative and Positive Feedback Circuits Along with Non-ERK Pathway Effects

    E-Print Network [OSTI]

    Kreeger, Pamela K.

    More than 40% of colon cancers have a mutation in K-RAS or N-RAS, GTPases that operate as central hubs for multiple key signaling pathways within the cell. Utilizing an isogenic panel of colon carcinoma cells with K-RAS ...

  11. Wrought iron hand tools in Port Royal, Jamaica: a study based upon a collection of the tools recovered from archaeological excavations and on tools listed in the probate inventories of colonial Port Royal, c. 1692

    E-Print Network [OSTI]

    Franklin, Marianne

    1992-01-01T23:59:59.000Z

    WROUGHT IRON HAND TOOLS IN PORT ROYAL, JAMAICA: A STUDY BASED UPON A COLLECTION OF THE TOOLS RECOVERED FROM ARCHAEOLOGICAL EXCAVATIONS AND ON TOOLS LISTED IN THE PROBATE INVENTORIES OF COLONIAL PORT ROYAL, c. 1692 A Thesis by MARIANNE... BASED UPON A COLLECTION OF THE TOOLS RECOVERED FROM ARCHAEOLOGICAL EXCAVATIONS AND ON TOOLS LISTED IN THE PROBATE INVENTORIES OF COLONIAL PORT ROYAL, c. 1692 A Thesis by MARIANNE FRANKLIN Approved as to style and content by: nny L. Hamilton...

  12. The C. elegans class A synthetic multivulva genes inhibit ectopic RAS-mediated vulval development by tightly restricting expression of lin-3 EGF

    E-Print Network [OSTI]

    Saffer, Adam M

    2011-01-01T23:59:59.000Z

    The class A and B synthetic multivulva (synMuv) genes of C. elegans redundantly antagonize an EGF/Ras pathway to prevent ectopic vulval induction. The class B synMuv genes encode many proteins known to remodel chromatin ...

  13. Effect of docosahexaenoic acid on ras post-translational processing and localization in a transgenic mouse colonic cell line

    E-Print Network [OSTI]

    Collett, Esther Dick

    2000-01-01T23:59:59.000Z

    . . 45 CHAPTER VI SUMMARY AND FUTURE INVESTIGATIONS . . . . 50 LITERATURE CITED . . 53 APPENDIX 1 PREPARATION OF YAMC MEDIA AND PLATING OF YAMC-RAS CELLS. 63 APPENDIX 2 PASSAGING PROTOCOL APPENDIX 3 FREEZING MEDIA PREPARATION AND FREEZING PROTOCOL... and the supernatant taken as the cytosolic extract. For preparation of the membrane fraction, the pellet was resuspended in the above buffer containing Triton-X 100 at a final concentration of 1N and allowed to incubate for 30 minutes on ice and then centrifuged...

  14. Reduction of metastasis, cell invasion, and adhesion in mouse osteosarcoma by YM529/ONO-5920-induced blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathway

    SciTech Connect (OSTI)

    Tsubaki, Masanobu [Division of Pharmacotherapy, Kinki University School of Pharmacy, Kowakae, Higashi-Osaka 577-8502 (Japan)] [Division of Pharmacotherapy, Kinki University School of Pharmacy, Kowakae, Higashi-Osaka 577-8502 (Japan); Satou, Takao; Itoh, Tatsuki [Department of Pathology, Kinki University School of Medicine, Osakasayama, Osaka 589-8511 (Japan)] [Department of Pathology, Kinki University School of Medicine, Osakasayama, Osaka 589-8511 (Japan); Imano, Motohiro [Department of Surgery, Kinki University School of Medicine, Osakasayama, Osaka 589-8511 (Japan)] [Department of Surgery, Kinki University School of Medicine, Osakasayama, Osaka 589-8511 (Japan); Ogaki, Mitsuhiko [Department of Pharmacy, Higahiosaka City General Hospital, Higashi-osaka, Osaka 578-8588 (Japan)] [Department of Pharmacy, Higahiosaka City General Hospital, Higashi-osaka, Osaka 578-8588 (Japan); Yanae, Masashi [Division of Pharmacotherapy, Kinki University School of Pharmacy, Kowakae, Higashi-Osaka 577-8502 (Japan) [Division of Pharmacotherapy, Kinki University School of Pharmacy, Kowakae, Higashi-Osaka 577-8502 (Japan); Depeartment of Pharmacy, Sakai Hospital, Kinki University School of Medicine, Sakai, Osaka 590-0132 (Japan); Nishida, Shozo, E-mail: nishida@phar.kindai.ac.jp [Division of Pharmacotherapy, Kinki University School of Pharmacy, Kowakae, Higashi-Osaka 577-8502 (Japan)] [Division of Pharmacotherapy, Kinki University School of Pharmacy, Kowakae, Higashi-Osaka 577-8502 (Japan)

    2012-03-15T23:59:59.000Z

    Osteosarcoma is one of the most common primary malignant bone tumors in children and adolescents. Some patients continue to have a poor prognosis, because of the metastatic disease. YM529/ONO-5920 is a nitrogen-containing bisphosphonate that has been used for the treatment of osteoporosis. YM529/ONO-5920 has recently been reported to induce apoptosis in various tumors including osteosarcoma. However, the mode of metastasis suppression in osteosarcoma by YM529/ONO-5920 is unclear. In the present study, we investigated whether YM529/ONO-5920 inhibited tumor cell migration, invasion, adhesion, or metastasis in the LM8 mouse osteosarcoma cell line. We found that YM529/ONO-5920 significantly inhibited metastasis, cell migration, invasion, and adhesion at concentrations that did not have antiproliferative effects on LM8 cells. YM529/ONO-5920 also inhibited the mRNA expression and protein activities of matrix metalloproteinases (MMPs). In addition, YM529/ONO-5920 suppressed phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and the serine/threonine protein kinase B (Akt) by the inhibition of Ras prenylation. Moreover, U0126, a mitogen-activated protein kinase kinase (MEK) 1/2 inhibitor, and LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, also inhibited LM8 cell migration, invasion, adhesion, and metastasis, as well as the mRNA expression and protein activities of MMP-1, MMP-2, MMP-9, and MT1-MMP. The results indicated that YM529/ONO-5920 suppressed the Ras/MEK/ERK and Ras/PI3K/Akt pathways, thereby inhibiting LM8 cell migration, invasion, adhesion, and metastasis. These findings suggest that YM529/ONO-5920 has potential clinical applications for the treatment of tumor cell metastasis in osteosarcoma. -- Highlights: ? We investigated whether YM529/ONO-5920 inhibited tumor metastasis in osteosarcoma. ? YM529/ONO-5920 inhibited metastasis, cell migration, invasion, and adhesion. ? YM529/ONO-5920 suppressed Ras signalings. ? YM529/ONO-5920 has potential clinical applications for the treatment in osteosarcoma.

  15. Time-resolved FTIR spectroscopy for monitoring protein dynamics exemplified by functional studies of Ras protein bound to a lipid bilayer

    E-Print Network [OSTI]

    Gerwert, Klaus

    bilayers can be investigated in near physiological con- ditions with the attenuated total reflection (ATR online 22 August 2011 Keywords: Infrared Time-resolved Difference spectroscopy Rapid scan Step scan GTPases GTP Caged-substances Ras Isotopic labeling Band assignment Global fit Attenuated total reflection

  16. Lead acetate induces EGFR activation upstream of SFK and PKC{alpha} linkage to the Ras/Raf-1/ERK signaling

    SciTech Connect (OSTI)

    Wang, C.-Y.; Wang, Y.-T.; Tzeng, D.-W. [Molecular Carcinogenesis Laboratory, Institute of Biotechnology and Department of Life Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan (China); Yang, J.-L. [Molecular Carcinogenesis Laboratory, Institute of Biotechnology and Department of Life Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan (China)], E-mail: jlyang@life.nthu.edu.tw

    2009-03-01T23:59:59.000Z

    Lead acetate (Pb), a probable human carcinogen, can activate protein kinase C (PKC) upstream of extracellular signal-regulated kinase 1 and 2 (ERK1/2). Yet, it remains unclear whether Pb activation of PKC {yields} ERK1/2 involves receptor/non-receptor tyrosine kinases and the Ras signaling transducer. Here we demonstrate a novel mechanism elicited by Pb for transmitting ERK1/2 signaling in CL3 human non-small-cell lung adenocarcinoma cells. Pb induction of higher steady-state levels of Ras-GTP was essential for increasing phospho-Raf-1{sup S338} and phospho-ERK1/2. Pre-treatment of the cells with a conventional PKC inhibitor Goe6976 or depleting PKC{alpha} using specific small interfering RNA blocked Pb induction of Ras-GTP. Pb also activated cellular tyrosine kinases. Specific pharmacological inhibitors, PD153035 for epidermal growth factor receptor (EGFR) and SU6656 for Src family tyrosine kinases (SFK), but not AG1296 for platelet-derived growth factor receptor, could suppress the Pb-induced tyrosine kinases, PKC{alpha}, Ras-GTP, phospho-Raf-1{sup S338} and phospho-ERK1/2. Furthermore, phosphorylation of tyrosines on the EGFR multiple autophosphorylation sites and the conserved SFK autophosphorylation site occurred during exposure of cells to Pb for 1-5 min and 5-30 min, respectively. Intriguingly, Pb activation of EGFR required the intrinsic kinase activity but not dimerization of the receptor. Inhibition of SFK or PKC{alpha} activities did not affect EGFR phosphorylation, while knockdown of EGFR blocked SFK phosphorylation and PKC{alpha} activation following Pb. Together, these results indicate that immediate activation of EGFR in response to Pb is obligatory for activation of SFK and PKC{alpha} and subsequent the Ras-Raf-1-MKK1/2-ERK1/2 signaling cascade.

  17. Extracellular signal-regulated kinase 2 (ERK-2) mediated phosphorylation regulates nucleo-cytoplasmic shuttling and cell growth control of Ras-associated tumor suppressor protein, RASSF2

    SciTech Connect (OSTI)

    Kumari, Gita [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India)] [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India); Mahalingam, S., E-mail: mahalingam@iitm.ac.in [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India); Department of Biotechnology, Laboratory of Molecular Virology and Cell Biology, Indian Institute of Technology-Madras, Chennai 600 036 (India)

    2009-10-01T23:59:59.000Z

    Ras GTPase controls the normal cell growth through binding with an array of effector molecules, such as Raf and PI3-kinase in a GTP-dependent manner. RASSF2, a member of the Ras association domain family, is known to be involved in the suppression of cell growth and is frequently down-regulated in various tumor tissues by promoter hypermethylation. In the present study, we demonstrate that RASSF2 shuttles between nucleus and cytoplasm by a signal-mediated process and its export from the nucleus is sensitive to leptomycin B. Amino acids between 240 to 260 in the C-terminus of RASSF2 harbor a functional nuclear export signal (NES), which is necessary and sufficient for efficient export of RASSF2 from the nucleus. Substitution of conserved Ile254, Val257 and Leu259 within the minimal NES impaired RASSF2 export from the nucleus. In addition, wild type but not the nuclear export defective RASSF2 mutant interacts with export receptor, CRM-1 and exported from the nucleus. Surprisingly, we observed nucleolar localization for the nuclear export defective mutant suggesting the possibility that RASSF2 may localize in different cellular compartments transiently in a cell cycle dependent manner and the observed nuclear localization for wild type protein may be due to faster export kinetics from the nucleolus. Furthermore, our data suggest that RASSF2 is specifically phosphorylated by MAPK/ERK-2 and the inhibitors of MAPK pathway impair the phosphorylation and subsequently block the export of RASSF2 from the nucleus. These data clearly suggest that ERK-2 mediated phosphorylation plays an important role in regulating the nucleo-cytoplasmic shuttling of RASSF2. Interestingly, nuclear import defective mutant of RASSF2 failed to induce cell cycle arrest at G1/S phase and apoptosis suggesting that RASSF2 regulates cell growth in a nuclear localization dependent manner. Collectively, these data provided evidence for the first time that MAPK/ERK-2 mediated phosphorylation regulates nucleo-cytoplasmic transport and cell growth arrest activity of RASSF2. Taken together, the present study suggests that active transport between nucleus and cytoplasm may constitute an important regulatory mechanism for RASSF2 function.

  18. Port Royal, Jamaica, and the slave trade

    E-Print Network [OSTI]

    Johnson, David A

    2000-01-01T23:59:59.000Z

    of the eighteenth century in the Florida Keys, Henrietta Marie had just quit Port Royal after off-loading her human cargo. The artifact collection of shackles, ship's equipment and trade goods represents the trappings of the maritime slave trade. Particularly...

  19. Tempo Excursions from Java to Jamaica

    E-Print Network [OSTI]

    Condit-Schultz, Nathaniel

    2012-01-01T23:59:59.000Z

    reaching all the way down to 40 bpm which is equivalent toIn the score the piece begins its spiral at 80 bpm but slowsto 56 bpm by measure 49 and then stays relatively stable for

  20. Petroleum Corporation of Jamaica | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroupPerfectenergy International Limited JumpPetbow Cogeneration LtdPetroleum

  1. Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras–Raf–ERK–Sp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei [Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan (China)] [Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan (China); Huang, Yuan-Li [Department of Biotechnology, College of Health Science, Asia University, Taichung 413, Taiwan (China)] [Department of Biotechnology, College of Health Science, Asia University, Taichung 413, Taiwan (China); Wong, Zong-Ruei; Su, Peng-Han [Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan (China)] [Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan (China); Huang, Bu-Miin [Department of Cell Biology and Anatomy, National Cheng-Kung University, Tainan 701, Taiwan (China)] [Department of Cell Biology and Anatomy, National Cheng-Kung University, Tainan 701, Taiwan (China); Ju, Tsai-Kai [Instrumentation Center, National Taiwan University, Taipei 106, Taiwan (China) [Instrumentation Center, National Taiwan University, Taipei 106, Taiwan (China); Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan (China); Yang, Hsi-Yuan, E-mail: hyhy@ntu.edu.tw [Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan (China)] [Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan (China)

    2013-05-17T23:59:59.000Z

    Highlights: •Nestin expression in C6 glioma cells is induced by FGF-2. •Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. •The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. •The mRNA of FGFR1 and 3 are detected in C6 glioma cells. •Ras–Raf–ERK–Sp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFR–MAPK–ERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  2. RAS Update December 2011 Page 1 RAS Update, December 2011

    E-Print Network [OSTI]

    Heller, Eric

    Administration Consulting Services Now Available New Science Division Website Provides Research Development Resources for Researchers New Federal Guidance on Accelerated ARRA Spending Revised Participation Woodward Massey with any questions. Back to top New Science Division Website Provides Research Development

  3. RAS Update January 2012 Page 1 RAS Update, January 2012

    E-Print Network [OSTI]

    Heller, Eric

    Changes Research Development: Faculty Science Management Series Overview Managing Your Research Revision information on the following: Welcome to Our New Colleagues Upcoming Research Administration Events Studies Workshop: Spending Sponsored Funds ­ Wednesday, March 7th Upcoming Funding Opportunities Packard

  4. Pewter and pewterers from Port Royal, Jamaica: flatware before 1692

    E-Print Network [OSTI]

    Gotelipe-Miller, Shirley

    1990-01-01T23:59:59.000Z

    Figure 2: The INA/TAMU Archaeological Excavations at Port Royal Figure 3: Detail of ar i e a la Mod by William Hogarth Figure 4: Der Kandelgiesser (The Pewterer) by P. Abraham Figure 5: Scrap Pewter Recovered from INA/TAMU Excavations Figure 6: Array... Land Holdings in Port Royal Table 3: Childermas Family Tree Table fn Benning Land Holdings in Port Royal Table 5: Benning Family Tree Table 6: Benning Family Timeline Table 7: Comparison of Probate Inventories Table 8: Comparison of Prices derived...

  5. Jamaica-Enhancing Capacity for Low Emission Development Strategies...

    Open Energy Info (EERE)

    initiative to support developing countries' efforts to pursue long-term, transformative development and accelerate sustainable, climate-resilient economic growth while slowing...

  6. Pewter and pewterers from Port Royal, Jamaica: flatware before 1692 

    E-Print Network [OSTI]

    Gotelipe-Miller, Shirley

    1990-01-01T23:59:59.000Z

    Figure 2: The INA/TAMU Archaeological Excavations at Port Royal Figure 3: Detail of ar i e a la Mod by William Hogarth Figure 4: Der Kandelgiesser (The Pewterer) by P. Abraham Figure 5: Scrap Pewter Recovered from INA/TAMU Excavations Figure 6: Array... Land Holdings in Port Royal Table 3: Childermas Family Tree Table fn Benning Land Holdings in Port Royal Table 5: Benning Family Tree Table 6: Benning Family Timeline Table 7: Comparison of Probate Inventories Table 8: Comparison of Prices derived...

  7. Jamaica Beach, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio: EnergyJalles

  8. Jamaica-Caribbean Community (CARICOM) Sustainable Energy Roadmap and

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio: EnergyJallesStrategy |

  9. Jamaica-Climate Technology Initiative Private Financing Advisory Network

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio: EnergyJallesStrategy

  10. Jamaica-Regional Implementation Plan for CARICOM's Climate Change

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:

  11. Jamaica-Pilot Program for Climate Resilience (PPCR) | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano, Hawaii | Wind Farm Jump to:Oregon:JadooInformation

  12. Jamaica-Enhancing Capacity for Low Emission Development Strategies

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup | OpenHunanInformation sourceInvensysIslandJackson(EC-LEDS) | Open

  13. Jamaica-IAEA Energy Planning | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup | OpenHunanInformation sourceInvensysIslandJackson(EC-LEDS) |

  14. China Energy Databook -- User Guide and Documentation, Version 7.0

    E-Print Network [OSTI]

    Fridley, Ed., David

    2008-01-01T23:59:59.000Z

    Jamaica Japan Jordan Kazakhstan Kenya Korea (DPRK) Korea (Jamaica Japan Jordan Kazakhstan Kenya Korea (DPRK) Korea (Jamaica Japan Jordan Kazakhstan Kenya Korea (DPRK) Korea (

  15. Constitutional Environment and Entrepreneurship: An Empirical Study

    E-Print Network [OSTI]

    Zhang, Wei

    2012-01-01T23:59:59.000Z

    outcomes. Jamaica Korea (S) Kyrgyzstan Lithuania MadagascarIsrael Jamaica Korea (S) Kyrgyzstan Lithuania MadagascarIsrael Jamaica Korea (S) Kyrgyzstan Lithuania Madagascar

  16. Astrium Satellites UK Lunar Polar Lander meeting, RAS

    E-Print Network [OSTI]

    Anand, Mahesh

    Propulsion Systems Mechanisms ExoMars Rover Portsmouth: Radar Systems -> Landing Passive Microwave 2018 Under development In orbit Exosat 1983 Hipparcos 1989 Solar Orbiter Planetary Science SolarAstrium.Itshallnotbecommunicatedtothirdpartieswithoutpriorwrittenagreement.Itscontentshallnotbedisclosed. Concept Design Based on these trade-results 2 concepts have been selected for further design Option 1

  17. automation society ras: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Engineering (T-ASE) February 2003: First Issue to appear in mid 2004. Executive Summary Automation plays an increasingly important role in the global economy and in our daily...

  18. activated ras alters: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in chronic human spinal cord injury Katherine M. Deutscha, b, T. George Hornbya, c, Brian D joint torques at the ankle, knee and hip. The results showed that SCI individuals...

  19. Characterization of a putative novel Ras effector in Dictyostelium discoideum

    E-Print Network [OSTI]

    Taylor, Natalie Marie

    2008-01-01T23:59:59.000Z

    pellet and supernatant samples were separated on SDS- PAGE and analyzed with Coomassie staining. Mass Production

  20. Aberrant Ras/MAPK signaling in skeletal development

    E-Print Network [OSTI]

    Nedelcu, Simona

    2013-01-01T23:59:59.000Z

    The Mitogen-activated protein kinase(MAPK) signaling pathway has been studied intensively in the context of neoplastic transformation. Other studies have focused on the roles of this pathway during development and have ...

  1. assessment scale ras: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kundur two area test system which exhibits an interarea oscillatory mode. Key words: Remedial action Venkatasubramanian, Mani V. 47 Self, Society, and Environment in the 21st...

  2. INSTITUTE OF APPLIED PHYSICS RAS RUSSIA NIZHNY NOVGOROD

    E-Print Network [OSTI]

    Fominov, Yakov

    ) The common features of electromagnetic pulses dynamics were investigated in the case of Kerr type nonlinearity. The common features of electromagnetic pulses dynamics were investigated in the case of Kerr type from the area occupied by electromagnetic laser pulse could be neglected quasi-flat model

  3. Agricultural Livelihoods and Climate Change: Employing the Livelihood Vulnerability Index in Bluefields, Jamaica

    E-Print Network [OSTI]

    Fath, Kevin

    2014-08-20T23:59:59.000Z

    in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Chair of Committee, Gary Wingenbach Committee Members, Theresa P. Murphrey Leonardo Lombardini Head of Department, John Elliot December 2014 Major Subject...; (c) health; (d) social networks; (e) food; (f) water; and (g) natural disasters and climate variability (Hahn et al., 2009). The Hahn et al. (2009) study took this vulnerability framework a step further by determining which secondary components...

  4. ENROLLED WSU INTERNATIONAL STUDENTS 1 Albania 1 35 Jamaica 2 69 Saudi Arabia 75

    E-Print Network [OSTI]

    Collins, Gary S.

    Republic 1 53 New Zealand 2 87 United Arab Emirates 6 20 Dominican Republic 3 54 Nigeria 5 88 United

  5. Attachment B: Jamaica Bay Watershed Protection Plan Update 1. WATER QUALITY

    E-Print Network [OSTI]

    Columbia University

    excess algae and sea lettuce to reduce nitrogen and produce biodiesel fuels. Design anticipated to begin Reintroduction of Eel Grass (Zostera marina) Pilot Study Fish habitat/Decreased wetland erosion loss. Design and increase fish habitat. Design anticipated to begin Summer 2008. Contractor by mid-2008; initiate pilot

  6. Historical analysis of pewter spoons recovered from the sunken city of Port Royal, Jamaica

    E-Print Network [OSTI]

    Wadley, Cathryn Ann

    1985-01-01T23:59:59.000Z

    Theophilus Presbyter, writing around A. D. 1100, described the casting of a cruet in a clay mould. First, a core was made out of clay. This core was coated with several thin layers of kneaded wax three to four mm thick. Details were then modelled...

  7. Wrought iron hand tools from the underwater archaelogical excavations of colonial Port Royal, Jamaica, C. 1692

    E-Print Network [OSTI]

    Custer, Katie Michelle

    2004-01-01T23:59:59.000Z

    was brought back to life and interpreted. A large collection of iron hand tools from this site was preserved. These conserved and replicated artifacts are now available for analysis. This thesis is a continuation of the catalog of hand tools from...

  8. Jamaica-Designing and Communicating Low Carbon Energy Roadmaps for Small

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano, Hawaii | Wind Farm Jump to:Oregon:Jadoo

  9. Jamaica-Low-Carbon Energy Roadmaps for the Greater Antilles | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup | OpenHunanInformation sourceInvensysIslandJackson(EC-LEDS)

  10. Society to 2050 AD: Anthropological Forecasts Extrapolating Correlates of Modernization

    E-Print Network [OSTI]

    Denton, Trevor

    2008-01-01T23:59:59.000Z

    Jamaica Japan Jordan Kazakhstan Kenya Kiribati Dev elop V1Italy Jamaica Japan Jordan Kazakhstan Kenya Kiribati Soc NumJamaica Japan Jordan Kazakhstan Kenya Kiribati V678 Rec V744

  11. International reserves management and the current account

    E-Print Network [OSTI]

    Aizenman, Joshua

    2007-01-01T23:59:59.000Z

    Guyana Indonesia Jordan Kazakhstan Kyrgyz Republic Laoof Iraq Jamaica Jordan Kazakhstan Macedonia, FYR MaldivesJamaica Japan Jordan Kazakhstan Kenya Korea Kuwait Kyrgyz

  12. The Right to Life with Dignity: Economic and Social Rights Respect in the World

    E-Print Network [OSTI]

    Kolp, Felicity Ann

    2010-01-01T23:59:59.000Z

    Italy Jamaica Japan Jordan Kazakhstan Kenya Korea, Dem. Rep.New Zealand Iceland Kazakhstan Korea, Dem. Rep. BulgariaChina Croatia Grenada Jamaica Kazakhstan Korea, Rep. Lebanon

  13. A Study of the Yield of Camphor and of Camphor Oil Obtained from the Laurus Camphora of Jamaica

    E-Print Network [OSTI]

    Weidlein, Edward Ray

    1910-05-15T23:59:59.000Z

    to the air* On fractionating this a color­ less -transparent liquid is obtained, specific gravity 870-©3.0. It boils from 150° to 195©. The chief con­ stituents are pinene, phellandrene, cineol, dipentene and some oamphor. On further fractionating, a yellow... of drying and distilling it is not possible to separate it from water and the essential oil, exoept with a loss of some of the camphor. We have thought it best, therefore, to follow a method which may be like the one used in Industrial extraction...

  14. FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans

    E-Print Network [OSTI]

    Lu, Jiamiao; Dentler, William L., Jr; Lundquist, Erik A.

    2008-05-16T23:59:59.000Z

    for the germ line morphogenesis defect by DIC optics and DAPI staining (Figure 11A) [30,32,33]. The hypomorphic loss-of-function allele n2021 caused a ky535-like germ line defect in 44% of gonad arms, and the stronger let-60 loss-of-function alleles s1124, s...1045), let-23(sy10), lin-31(n301). LGIII: fli-1(ky535), fli-1(tm362), tnIs6 [plim-7::gfp], dpy- 17(e164), unc-32(e189), mpk-1(ku1), eT1. LGIV: let- 60(n2021), let-60(s1124), let-60(s1155), let-60(s59), let- 60(sy93), let-60(sy92), let-60(sy99), let-60(n...

  15. Ras-association domain family 1C protein promotes breast cancer cell migration and attenuates apoptosis

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    wells containing either 40 ng/ml SDF-1 con- ditioned mediumor control medium lacking SDF-1 and incubated for 22 hours.and grown in the presence of SDF-1 (the ligand for the CXCR4

  16. Institutions of the Russian Academy of Sciences Joint Institute for High Temperatures RAS

    E-Print Network [OSTI]

    Fominov, Yakov

    and temperatures, low-temperature plasma physics, issues of physics and power engineering, as well as technology of z-pinch dis

  17. 1. PMU Based RAS for a Hybrid AC/DC System 1.1. Project Team

    E-Print Network [OSTI]

    Li, Husheng

    to minimize the impact of disturbances. It is also an integration of various areas related to CPS these wide area protection schemes. Such schemes are essential to the strategic plan in enabling robustness

  18. Context-Dependent Transformation of Adult Pancreatic Cells by Oncogenic K-Ras

    E-Print Network [OSTI]

    Friedlander, Sharon Y. Gidekel

    Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human malignancies. To investigate the cellular origin(s) of this cancer, we determined the effect of PDAC-relevant gene mutations in distinct cell types ...

  19. Wild Rose SFH DNR's largest facility Total Replacement Project Engineering Study RAS Intensive Rearing

    E-Print Network [OSTI]

    liquid oxygen tank #12;Remote controls for production wells in coldwater headtank building (HDR Senior 2,400 gpm 75 deg F 2-Stage Water Heating Heat Recovery Finish Heating #12;Moving Bed Biofilter & K1

  20. RasMol v2.5 A Molecular Visualisation Program

    E-Print Network [OSTI]

    Meagher, Mary

    Incorporated. Alchemy and Sybyl are trademarks of Tripos Associates Inc. VAX is a trademark of Digital&S workstations, DEC Alpha (OSF/1, OpenVMS and Windows NT), IBM RS/6000, Cray, Sequent, VAX VMS (under DEC windows

  1. activated h-ras-1 oncogene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: recently demonstrated that the oncogenic, constitutively kinase activated NPM-ALK protein was antiapoptotic identified as part of the NPM-ALK oncogenic fusion...

  2. Ras-association domain family 1C protein promotes breast cancer cell migration and attenuates apoptosis

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    breast cancer cell lines (MDA-MB231 and T47D) caused a smallin breast cancer cells (MDA-MB231 and T47D) resulted in a3H-Thymidine incorporation MDA-MB231, T47D, and AG1132B

  3. Anti-tumour activity in RAS-driven tumours by blocking AKT and MEK

    E-Print Network [OSTI]

    Tolcher, Anthony W.; Khan, Khurum; Ong, Michael; Banerji, Udai; Papadimitrakopoulou, Vassiliki; Gandara, David; Patnaik, Amita; Baird, Richard D.; Olmos, David; Garrett, Christopher R.; Skolnik, Jeffrey M.; Rubin, Eric; Smith, Paul; Huang, Pearl; Learoyd, Maria; Shannon, Keith; Morosky, Anne; Tetteh, Ernestina; Jou, Ying-Ming; Papadopoulos, Kyriakos P.; Moreno, Victor; Kaiser, Brianne; Yap, Timothy A.; Yan, Li; de Bono, Johann S.

    2014-12-16T23:59:59.000Z

    , MD, PhD,2 David Olmos, MD, PhD,2 Christopher R. Garrett, MD,3 Jeffrey M. Skolnik, MD,5 Eric Rubin, MD,6 Paul Smith, PhD,5 Pearl Huang, PhD,6 Maria Learoyd, PhD,5 Keith Shannon, MS,6 Anne Morosky, RN, BSN,6 Ernestina Tetteh, PhD,6 Ying-Ming Jou, PhD,6... Determining the combination index by the Chou-Talalay method for cell lines treated with selumetinib and MK-2206 Combinations of MK-2206 with AZD6244 were evaluated in 9 cancer cell lines with various mutational activations in the PI3K and/or MAPK pathways: 3...

  4. Energy Department Sends First Energy Saving Team to Federal Government...

    Office of Environmental Management (EM)

    Sends First Energy Saving Team to Federal Government Site in Jamaica, N.Y. Energy Department Sends First Energy Saving Team to Federal Government Site in Jamaica, N.Y. October 27,...

  5. Exhuming caliban : gothic and madness in late twentieth and twenty-first - century Caribbean literary fictions

    E-Print Network [OSTI]

    Escudero, Andrew Mario

    2012-01-01T23:59:59.000Z

    Horse: Voodoo and Life in Haiti and Jamaica. Ed. Henry LouisHorse: Voodoo and Life in Haiti and Jamaica, Ralph Ellison’sdeveloping nations” such as Haiti, Mexico and Ghana, and

  6. The role of the artist in a developing neighbourhood garden : the case of Dungarven Rd. lot in the community of Jamaica Plain, MA

    E-Print Network [OSTI]

    Smith, David Brian

    1997-01-01T23:59:59.000Z

    An exploration of the role of the artist in a developing neighbourhood garden through the examination of the following topics: wilderness, landscape, gardens, community gardens and various artists' work in the land. Leading ...

  7. E. arth In the Balance: Sustainability in Jamaica This summer, the College of EMS had the unprecedented opportunity to offer twenty-

    E-Print Network [OSTI]

    Thompson, Anne

    , introduces students to energy production extraction technologies and the impact of energy production on the environment. Understanding basic concepts of energy production and use helps students better understand

  8. International Student and Scholar Enrollment & Statistical Report

    E-Print Network [OSTI]

    Ginzel, Matthew

    Jamaica 8 Ukraine 11 Japan 57 United Arab Emirates 20 Jordan 45 United Kingdom 33 Kazakhstan 72 Uzbekistan

  9. International Student and Scholar Enrollment & Statistical Report

    E-Print Network [OSTI]

    Ginzel, Matthew

    Ukraine 16 Israel 4 United Arab Emirates 20 Italy 13 United Kingdom 28 Jamaica 11 Venezuela 12 Japan 71

  10. International Student and Scholar Enrollment & Statistical Report

    E-Print Network [OSTI]

    Ginzel, Matthew

    6 Uganda 5 Israel 5 Ukraine 14 Italy 16 United Arab Emirates 21 Jamaica 7 United Kingdom 28 Japan 57

  11. 16 Evolutionary Diversification of Caribbean Anolis Lizards 335 To what extent does this interisland study of size offer evidence for the role

    E-Print Network [OSTI]

    Losos, Jonathan B.

    diversification, focusing primarily on the anoles of the Greater Antilles (Cuba, Hispaniola, Jamaica, and Puerto of Speciation Bahamas Cuba Jamaica Hispaniola Puerto Rico Lesser Antilles 500 km C arib b ean Sea A tlan tic O cean Jamaica (6)* Mainland Cuba (15) Hispaniola (1) Cuba (4) Cuba (4)* Cuba(4) Cuba (14) Hispaniola (8

  12. SCIENTIFIC ADVISORY COMMITTEE A.N. Skrinsky (chairman), Novosibirsk

    E-Print Network [OSTI]

    , Moscow V.M. Fomin, ITAM SB RAS, Novosibirsk M.V. Kovalchuk, Institute of Crystallography RAS, Moscow G

  13. Determining the optimal river gauge location for a flood early warning system in Uganda using HEC-RAS and AHP

    E-Print Network [OSTI]

    Cheung, Joyce, M. Eng. Massachusetts Institute of Technology

    2014-01-01T23:59:59.000Z

    Flooding of the Manafwa River in Eastern Uganda causes significant damage in the district of Butaleja, and often occurs without advance warning. In 2012, the American Red Cross in Uganda requested MIT to develop a flood ...

  14. ORIGINAL ARTICLE B-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase

    E-Print Network [OSTI]

    ERK1 and ERK2 (extracellular signal-regulated kinases 1 and 2) are probably the best studied and best understood of the mitogen-activated protein kinases (MAPKs). ERK1 and ERK2 respond to diverse stimuli activators are the kinases MEK1 and MEK2 (for MAPK kinase or ERK kinase), which phosphorylate ERK1 and ERK2

  15. Ethiopia and Eritrea During the Scramble for Africa: A Political Biography of Ras Alulá, 1875-1897

    E-Print Network [OSTI]

    Demissie, Fassil

    1983-01-01T23:59:59.000Z

    and Faber, London, 1974. Ethiopia and Eritrea During thediplomatic and military history of contemporary Ethiopia.this crucial juncture, Ethiopia faced three well organized

  16. & Science Service F a t w e Released upon receipt

    E-Print Network [OSTI]

    to have been responsible f o r the disastrous deluge of r a i n that descended on eastern Jamaica during

  17. accidental expositions proposta: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    IN CHILDREN IN JAMAICA AND BELFAST: A COMPARATIVE STUDY CiteSeer Summary: IN EUROPEAN cities accidental poisoning has been a matter of increasing concern. The incidence...

  18. --No Title--

    Annual Energy Outlook 2013 [U.S. Energy Information Administration (EIA)]

    Iceland India Indonesia Iran Iraq Ireland Israel Italy Jamaica Japan Jordan Kazakhstan Kenya Kiribati Korea, North Korea, South Kuwait Kyrgyzstan Laos Latvia Lebanon Lesotho...

  19. Essays on the politics of regulation

    E-Print Network [OSTI]

    Weymouth, Stephen

    2010-01-01T23:59:59.000Z

    Iran Israel Jamaica Jordan Kazakhstan Kenya Kuwait KyrgyzHungary Tajikistan Romania Kazakhstan Senegal Czech RepublicBosnia and Herzegovina Kazakhstan Turkey Poland Belarus

  20. Essays in Open Economy Monetary Policy

    E-Print Network [OSTI]

    Castro, Pedro

    2012-01-01T23:59:59.000Z

    Croatia Jordan Denmark Kazakhstan Dominican Republic EgyptJamaica Japan Jordan Kazakhstan Latvia Lebanon MauritiusEgypt India Jordan Kazakhstan Pakistan Russia Tunisia

  1. area surrounding suez: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Also, I am grateful to Resource Chief Brian Kenner 5 Jamaica Bay BioBlitz 2010 Floyd Bennett Field and Surrounding Areas Environmental Sciences and Ecology Websites Summary:...

  2. Crimes against Humanity at the Extraordinary Chambers in the Courts of Cambodia: Is a Connection with Armed Conflict Required?

    E-Print Network [OSTI]

    Ford, Stuart

    2007-01-01T23:59:59.000Z

    India, Kenya, Mexico, Mongolia, Nigeria, Philippines,Jamaica, Jordan, Ke- nya, Mongolia, Nigeria, Oman, Panama,Id. at 279. In 1985, Mongolia also blamed the "increased

  3. Explaining the causes and consequences of internationally monitored elections

    E-Print Network [OSTI]

    Hyde, Susan Dayton

    2006-01-01T23:59:59.000Z

    Maldives San Marino Bahamas Malta Sao Tome & PrincipePapua New Guinea, Bahamas*, Malta* Bolivia, Cyprus, Jamaica,Slovenia, Cape Verde*, Malta* Poland, Slovenia, Mongolia,

  4. Aluminum across the Americas: Caribbean Mobilities and Transnational American Studies

    E-Print Network [OSTI]

    Sheller, Mimi

    2013-01-01T23:59:59.000Z

    Modernity Disavowed: Haiti and the Cultures of Slavery inAmerica Rises Against Them,” Haiti Analysis, 27 July 2012,and Peasant Radicalism in Haiti and Jamaica. London and

  5. Aluminum across the Americas: Caribbean Mobilities and Transnational American Studies

    E-Print Network [OSTI]

    Sheller, Mimi

    2013-01-01T23:59:59.000Z

    E. Jamaica in the World Aluminum Industry, 1838–1973, Vol.2007. Doordan, Dennis. “Promoting Aluminum: Designers andthe American Aluminum Industry. ” Design Issues 9, no. 2 (

  6. Tissue-specific interactions between oncogenic K-ras and the p19A?r?f?_p53 pathway determine susceptibility to transformation

    E-Print Network [OSTI]

    Young, Nathan Price

    2010-01-01T23:59:59.000Z

    Tumor development is a multi-step process driven by the collective action of gain-of-function mutations in oncogenes and loss-of-function alterations in tumor suppressor genes. The particular spectrum of mutations in a ...

  7. The pro-apoptotic K-Ras 4A proto-oncoprotein does not affect tumorigenesis in the ApcMin/+ mouse small intestine

    E-Print Network [OSTI]

    Patek, Charles E.; Arends, Mark J.; Rose, Lorraine; Luo, Feijun; Walker, Marion; Devenney, Paul S.; Berry, Rachel L.; Lawrence, Nicola J.; Ridgway, Rachel A.; Sansom, Owen J.; Hooper, Martin L.

    2008-06-13T23:59:59.000Z

    -actin transcripts were 185 bp, 158 bp and 148 bp respectively. The specificities of the PCR reactions were confirmed by dissociation curve analysis and 2% agarose gel electrophoresis. All PCR products were analysed when in the exponential phase of PCR amplification... of uncensored observations. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0 50 100 150 200 250 300 350 400 Age (days) K ap la n- M ei er s ur vi va l f un ct io nPage 6 of 9 (page number not for citation purposes) complex relationship between apoptosis and cancer...

  8. CHARTER ON LANGUAGE POLICY AND LANGUAGE RIGHTS INTHE CREOLE-SPEAKING CARIBBEAN

    E-Print Network [OSTI]

    Berdichevsky, Victor

    CHARTER ON LANGUAGE POLICY AND LANGUAGE RIGHTS INTHE CREOLE-SPEAKING CARIBBEAN Kingston, Jamaica-Speaking Caribbean January 2011 PREAMBLE The Creole-speaking Caribbean state parties to the Charter on Language Policy and Language Rights in the Creole-speaking Caribbean, meeting in Kingston, Jamaica from January 13

  9. THE ANOLES OF SOROA INTRODUCTION

    E-Print Network [OSTI]

    Losos, Jonathan B.

    ], Barbour and Ramsden 1919 [Cuba], Grant 1940 [Jamaica], Cochran 1941 [Hispaniola). Since 1941 investigation on Jamaica, about 46 described for Cuba, more than 40, some undescribed, on Hispaniola, 10 on the main island a sugar mill in Hispaniola; it was introduced there in the 19...s from Puerto Rico. A. porcatus, a native

  10. MFR PAPER 1087 Trap Fishing Explorations for Snapper

    E-Print Network [OSTI]

    lying south , southeast, and southwest of Jamaica ; north of Hispaniola; in the northern Leeward Islands, north of Hispaniola , and off Venezuela , Guyana, and Surinam. Higher catch rates of about 40 pounds per the Jamaica Banks were grunts , triggerfish, squirrelfish , and porgies taken as a group. North of Hispaniola

  11. Phytologia (April 2008) 90(1) 103 THE EVOLUTION OF CARIBBEAN JUNIPERUS

    E-Print Network [OSTI]

    Adams, Robert P.

    ; J. lucayana from Cuba, Haiti, Jamaica and the Bahamas; J. gracilior Pilger from Haiti and Dominican Republic; J. ekmanii Florin from Haiti; and J. urbaniana Pilger & Ekman from Haiti. Carabia (1941, Cuba, Jamaica, likely extinct in Haiti), J. gracilior (endemic to Hispaniola), J. gracilior var

  12. Constitutively Active Rap2 Transgenic Mice Display Fewer Dendritic Spines, Reduced Extracellular Signal-Regulated Kinase Signaling, Enhanced Long-Term Depression, and Impaired Spatial Learning and Fear Extinction

    E-Print Network [OSTI]

    Ryu, Jubin

    Within the Ras superfamily of GTPases, Rap1 and Rap2 are the closest homologs to Ras. In non-neural cells, Rap signaling can antagonize Ras signaling. In neurons, Rap also seems to oppose Ras in terms of synaptic function. ...

  13. Arnold Schwarzenegger ENERGY PERFORMANCE OF

    E-Print Network [OSTI]

    been focused on RAS for displacement ventilation systems, relatively little has been done to develop

  14. mE MEGALOPA STAGE OF mE GULF STONE CRAB, MENIPPE ADINA WILLIAMS AND FELDER, 1986, WIm A COMPARISON OF MEGALOPAE

    E-Print Network [OSTI]

    , the Bahamas, Cuba, and Jamaica (Rathbun 1930; Felder 1973; Williams 1984; Williams and Felder 1986). RecentlyMexico; hybridization ofthe two species occurs in northwest Florida (see Williams and Felder 1986). These two species

  15. International Student and Scholar Enrollment & Statistical Report

    E-Print Network [OSTI]

    Ginzel, Matthew

    EMIRATES3 UNITED ARAB 15 ISRAEL 3 UNITED KINGDOM 37 ITALY 1 Y4 URUGUA 1 JAMAICA 14 UZBEKISTAN 1 JAPAN 73 (20), Brazil (16) and United Kingdom (16) are the top ten sending countries for international faculty

  16. JPRS report: Nuclear developments, [February 25, 1988

    SciTech Connect (OSTI)

    NONE

    1988-02-25T23:59:59.000Z

    This report contains information concerning the nuclear developments of the following countries: Canada, Nigeria, China, Japan, Argentina, Brazil, Jamaica, Bangladesh, India, Pakistan, Egypt, Sudan, Ireland, France, Turkey, United Kingdom, and Federal Republic of Germany.

  17. Alternative Energy Technologies Solar Power

    E-Print Network [OSTI]

    Scott, Christopher

    #12;Alternative Energy Technologies Solar Power Photovoltaics Concentrating Solar Power (CSP) Power;Concentrating Solar Power (CSP) Reflector material is Aluminum or Silver Tube material ..... Several possible ............... Mexico, Canada, Peru Alumina ............Guinea, Brazil, Australia, Jamaica Manganese ....... S. Africa

  18. Cornell University, Office of Sponsored Programs

    E-Print Network [OSTI]

    Lipson, Michal

    GROWTH CHAMBERS; GOOD AGRICULTURAL PRACTICES; AND PLANT BREEDING 71373 $150,000.00 #12;BUTCHER, JONATHAN Natural Resources CUNY RESILIENCE PRACTICE IN JAMAICA BAY AND ENVIRONS: A PROPOSAL TO HELP LAUNCH

  19. November 20-December 3, 20086 www.harvard.edu Vol. CIV No. 11 Harvard University

    E-Print Network [OSTI]

    literature by reading from his poetry. Page 15 Money and God Scholar talks about the `prosperity gospel' -- and a slum in Kingston, Jamaica. Page 13 125th Game Read the curious history of the great- est game of all

  20. S. B. Hedges Electronic supplementary material

    E-Print Network [OSTI]

    Hedges, Blair

    del mondo, the prints of Cuba and Hispaniola were selected because some were available for analysis). Of the two, only Hispaniola showed some retouching in the last (1620) edition. The Porcacchi map of Jamaica

  1. -Pp. 211-254 In, Duellman,W. E. (Ed.) Patterns of distribution of amphibians: A global perspective.

    E-Print Network [OSTI]

    Hedges, Blair

    ) in Jamaica, 63 species (100% endemic) in Hispaniola, 20 species (100% endemic) in the Puerto Rican Bank, Hispaniola, and Puerto Rico), the Lesser Antilles, the Bahamas Bank, the Cayman Islands, and San Andrks

  2. ORIGINAL ARTICLE doi:10.1111/j.1558-5646.2008.00538.x

    E-Print Network [OSTI]

    Wiens, John J.

    and Hispaniola are consistent with expectations from competition. Phylogenetic analyses show that similar body-size extremes in Jamaica and Hispaniola have originated through parallel evolution on each island, and the rate

  3. Zoogeography and systematics of the shallow water echinodermata of Texas

    E-Print Network [OSTI]

    Pomory, Christopher Mark

    1990-01-01T23:59:59.000Z

    Guatemala Honduras Nicarag Cos Oo Jamaica Haiti Guadeloupe Oa Dominica ~Martinique tySt Lucia +Barbados @&~St- Vincent Gpr nada Tobago rinidad Belize Puerto Rico Curacao Aru 1 Bonaire a ~ ia a Rica Venezuela Panama Anguilla St. Martin...

  4. arXiv:nucl-ex/0410035v122Oct2004 Structure and Fine Structure in Multiparticle Production

    E-Print Network [OSTI]

    that this is an expanded version of a talk that I gave at the 20th Winter Workshop on Nuclear Dynamics in Jamaica, 2004. I at High Energies Wit Busza Physics Department and Laboratory for Nuclear Science, Massachusetts Institute

  5. Conjuring Freedom: Reconstructions and Revisions of Neo-Slave Narratives

    E-Print Network [OSTI]

    Montgomery, Christine Lupo

    2012-01-01T23:59:59.000Z

    pp. 97. Dayan, Joan. Haiti, History, and the Gods.Horse: Voodoo and Life in Haiti and Jamaica. 1938. Reprint,Horsemen: The Living Gods of Haiti (1953; reprint, Kingston,

  6. 21st Century Zombies: New Media, Cinema, and Performance

    E-Print Network [OSTI]

    Taylor, Joanne Marie

    2011-01-01T23:59:59.000Z

    Métraux, Alfred. Voodoo in Haiti. Trans. Hugo Charteris. Newthe Law: Vodou and Power in Haiti. Chicago: University ofMy Horse: Voodoo and Life in Haiti and Jamaica. New York:

  7. Plantation states : region, race, and sexuality in the cultural memory of the U.S. South, 1900-1945

    E-Print Network [OSTI]

    Steeby, Elizabeth Anna

    2008-01-01T23:59:59.000Z

    My Horse: Voodoo Life in Haiti and Jamaica. New York: HarperThe U.S. Occupation of Haiti: 1915-1934. New Brunswick, NJ:The U.S. Occupation of Haiti: 1915-1934. 43 See Plummer,

  8. International Student Enrollment Report Report based on Active F-1 & J-1 International

    E-Print Network [OSTI]

    Bordenstein, Seth

    3 27 Thailand 2 13 Ireland 1 28 Turkey 3 14 Israel 2 29 United Arab Emirates 1 15 Jamaica 1 30 Iraq 2 84 Turkey 23 38 Ireland 1 85 Uganda 2 39 Israel 3 86 Ukraine 5 40 Italy 4 87 United Arab Emirates 1 41 Jamaica 7 88 United Kingdom 10 42 Japan 31 89 Uzbekistan 2 43 Jordan 1 90 Venezuela 5 44

  9. A cross-national study of the pattern of modernization in the developing countries, 1965-75

    E-Print Network [OSTI]

    Choi, Kwok-on Frankie

    1979-01-01T23:59:59.000Z

    COUNTRIES? 1965 AND 1973 (IN U. S. $) Countries 1965" GNP Rank 1 973+? GNP Rank Venezuela Argentina Greece Uraguay Chile Spain Libya Jamaica Panama Mexico Lebanon Costa Rica Portugal Peru Nicaragua Guatemala Malaysia Ghana Colombia... Portugal Greece Kenya (white pop. only) Lebanon Iran Jordan C olombia Jamaica Libya Brazil Malaysia Uraguay Paraguay Peru Mexico Syria Egypt Iraq Sri Lanka Costa Rica Panama Guatemala Ecuador South Korea Ghana Ethiopia Turkey India...

  10. We constructed a simple mathematical model to examine the relative effectiveness

    E-Print Network [OSTI]

    Desplan, Claude

    expression construct pFBE (10). tRe-Ras64BV12 was constructed by cloning the Ras64BV12 cDNA as an Eco RI

  11. Chapter 1 Purpose of and Need for Action

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For the SOA path, BPA and other utilities have developed a non-wires measure called a remedial action scheme (RAS) that is carried out when needed. RAS uses a high-speed...

  12. Differentiation Self-renewal

    E-Print Network [OSTI]

    Glyde, Henry R.

    Off state Ras Ras­GAP PI3K ERK1 ERK2 Differentiation SC1 SC1 Self-renewal Ras On state GDP GTP N NN, which promotes stem-cell self-renewal. Activated Ras also switches on the enzymes ERK1 and ERK2, which-cell renewal via the PI3K pathway. SC1 also inhibits ERK1 and ERK2, thus blocking stem-cell differentiation. PI

  13. An investigation of time variations in a subtropical jet stream and the associated cloud patterns as shown by TIROS I

    E-Print Network [OSTI]

    Lester, Peter Francis

    1964-01-01T23:59:59.000Z

    section of Figure 7 for 5 April 1960 at 0700 EST 10. Cross section, Jacksonville to Jamaica, for 5 April 1960 at 0700 EST 11. Cross section, Jacksonville to Jamaica, for 5 April 1960 at 1900 EST 12. Surface synoptic chart for 6 April 1960 at 1900... was considered to be accurate within 60 nm due to the presence of land marks in five of the six photos. The tracks of the satellite are illustrated in Figure 2. 10 For each day (5 April 1960-9 April 1960), the follow- ing charts were constructed: 1) Surface...

  14. 10th International Conference on Chemometrics in Analytical Chemistry CHEMOMETRICS IN THE TROPICS

    E-Print Network [OSTI]

    Ferreira, Márcia M. C.

    Russian: http://rcs.chph.ras.ru/rcsin.htm -in Croatian: http://www: Afrikaans: CHEMOMETRIE CHEMO- + -METRIE (60-90%) CHEMOMETRIKE CHEMO- + -METRIKA (10-40%) Croatian

  15. apoptotic neutrophils induced: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    oncogenic-RAS synthetic Stockwell, Brent R. 15 EDITORIAL How to regulate neutrophils in gout CiteSeer Summary: Most research in gout has concentrated on the proinflammatory...

  16. Molecular Ecology (2008) 17, 598610 doi: 10.1111/j.1365-294X.2007.03595.x 2007 The Authors

    E-Print Network [OSTI]

    Quaternary climate change had a strong impact on the geographical distribution and demographic history Ecology, Ural Division of RAS, Yekaterinburg, Russia, World Museum of Mammoth, Yakutsk 677007, Russia

  17. E-Print Network 3.0 - anatomy pathophysiology neuroradiology...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Anatomy for Physician Assistants I PAS 7001 Anatomy for Physician Assistants Lab PAS 7070 Health Care... Issues PAS 7500 Pathophysiology RAS 7400 Patient Assessment Semester Total...

  18. LANL: CPO: Los Alamos National Laboratory Impacts and Investments...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Top Of It Maintenance Service * Orange Tackle Corp. * Ortega's Weaving Shop * Otra Vuelta Tire Recycling * Party Creations & Supplies * Performance Maintenance Incorporated * RAS...

  19. Department of the Navy, DPA Presentation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2012 GGF DEMONSTRATION Great Green Fleet - RIMPAC 2012 1,800 hours of shipboard gas turbine operation 240 flight hours Four ship-to-ship RAS evolutions One...

  20. Breaking a Pocket of Resistance in the Fight Against Cancer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Rat Sarcoma, is a nucleotide binding protein that responds to chemical signals (nucleotides). When in the "on" state, RAS activates other proteins, resulting in a cascade of...

  1. Malcolm, N. and Aggarwal, R.k. (2014) An Analysis of Reducing Back Flashover Faults with Surge Arresters on 69/138 kV Double

    E-Print Network [OSTI]

    McCusker, Guy

    Arresters on 69/138 kV Double Circuit Transmission Lines Due to Direct Lightning Strikes on the Shield Wires-EMTP, Back flashover faults, Lightning strokes, Surge arrester, Transmission lines. Abstract Back flashover causes of power interruptions on the double-circuit 69/138 kV overhead transmission lines in Jamaica

  2. The University of Washington acts on its great capacity and ambition for addressing vital issues, motivated by the power of shared concern, as is

    E-Print Network [OSTI]

    Yetisgen-Yildiz, Meliha

    Personnel (AP) serves as the institutional portal for academic personnel administration. We bring clear transitions for faculty, librarians, and academic staff · Monitor and uphold the high standards for ethics AFGHANISTAN 1 ALBANIA 1 AZERBAIJAN 1 BULARIA 1 BURMA (MYANMAR) 1 CAMEROON 1 COSTA RICA 1 ETHIOPIA 1 JAMAICA 1

  3. The Education of Iris Macfarlane, 1922-1939

    E-Print Network [OSTI]

    Macfarlane, Alan

    2015-06-16T23:59:59.000Z

    had also been in Jamaica, and then returned to Scotland where Maria’s brother was educated and 5 became the first bishop of the Falkland islands. Others were in the navy and army and also had long connections with India. My great...

  4. Appropriate Technologies and Systems to respond to Climate Change, Improved Water Resources Management, Waste Management and Sanitation

    E-Print Network [OSTI]

    Barthelat, Francois

    Resources Management, Waste Management and Sanitation A Review of Water Information Systems in the English availability, quality, use and demand. In this regard, Water Information Systems play a key role in the management of the resource. This paper examines the water information systems of St. Lucia, Jamaica

  5. PROJECT INFORMATION Project Number: S61268-583

    E-Print Network [OSTI]

    Barthelat, Francois

    ANALYSIS Narrative summary Expected results Performance measurement Assumptions and level of risk Goal and decision-makers with tools for developing IWRM policies in three DC's (Grenada, Guyana, Jamaica) by Year 6, Grenada and Guyana are committed to achieving and sustaining project results. Level of Risk: Low 2

  6. Influence of the tropical Atlantic versus the tropical Pacific on Caribbean rainfall

    E-Print Network [OSTI]

    . Taylor Department of Physics, University of the West Indies, Mona, Jamaica David B. Enfield Physical); KEYWORDS: Caribbean, rainfall, El Nin~o, tropical Atlantic, warm pool, model Citation: Taylor, M. A., D. B the two equatorial oceanic basins. Oppositely signed SST anomalies in the NINO3 region and the central

  7. NEUROSCIENCE GRADUATE STUDENTS STUDENT BODY 2012-2013 NAME OFFICE # LAB # LAB Ext LAB PI E-MAIL

    E-Print Network [OSTI]

    Valero-Cuevas, Francisco

    , WENDY HNB 228 X 05473 MCKEMY wknowlto@usc.edu LEE, JOO-YEUN FIRST YEAR jaemunki@usc.edu LEUNG, BRIAN-CUEVAS raths@usc.edu RETTBERG, JAMAICA BRINTON rettberg@usc.edu RIEDEL, BRANDALYN bcriedel@usc.edu ROACH, SHANE

  8. S68 JUNE 2006| d. Central America and the Caribbean--E. K. Grover-Kopec31

    E-Print Network [OSTI]

    S68 JUNE 2006| d. Central America and the Caribbean--E. K. Grover-Kopec31 I) TEMPERATURE Annual in the Caribbean, particularly in Jamaica, eastern Cuba, and western Haiti. Drought conditions, which have had of the Caribbean and Central American region is bimodal, with relative maxima occurring in May­June and September

  9. Byggmeister Test Home: Cold Climate Multifamily Masonry Building Condition Assessment and Retrofit Analysis

    SciTech Connect (OSTI)

    Wytrykowska, H.; Ueno, K.; Van Straaten, R.

    2012-09-01T23:59:59.000Z

    This report describes a retrofit project undertaken by Building Science Corporation and partner Byggmeister on a multifamily brick row house located in Jamaica Plain, MA. This project studied the row house to determine the right combination of energy efficiency measures that are feasible, affordable, and suitable for this type of construction and acceptable to homeowners.

  10. Transactions of the 16th Caribbean Geological Conference, Barbados.

    E-Print Network [OSTI]

    Abbott Jr., Richard N.

    Hispaniola is one of the four large islands of the Greater Antilles (Fig. 1). Three of the islands (Jamaica, Hispaniola and Puerto Rico) lie just south of the northern edge of the Caribbean plate. The fourth of the Greater Antilles: C = Cuba; H = Hispaniola; J = Jama

  11. LEIOCEPHALIDAE 1989 Leiocephalinae Frost and Etheridge, Misc. Publ. Mus. Nat. Hist. Univ.

    E-Print Network [OSTI]

    , Hispaniola, Navassa, and Martinique. Recently extinct species were found on Barbuda and Antigua, Anguilla, Guadeloupe, Hispaniola, Puerto Rico, and Jamaica. Leiocephalus Gray 1827 Leiocephalus Gray, Philos. Mag, 84: 1. Range: Cuba and nearby islands, the Cayman Islands, the Bahama Islands, Hispaniola

  12. Caribbean Journal of Science, Vol. 26, No 1-2, 7-30, 1990 Copyright 1990 College of Arts and Sciences

    E-Print Network [OSTI]

    Hedges, Blair

    on Cuba, Jamaica, Hispaniola, and Puerto Rico, and found little evidence of close inter (grahami), Hispaniola (chlorocyanus, christophei, cuvieri, cybotes, darlingtoni, distichus, hen- dersoni on the North Island (Hispaniola) in the Eocene or Oligocene indicates an early arrival of the genus in the West

  13. Citizenship and Emancipation: Voting Rights during the Haitian Revolution after 1793

    E-Print Network [OSTI]

    Gaffney, Jennifer Ann

    2014-04-23T23:59:59.000Z

    , France possessed the most profitable colony in the world. By 1789, Saint-Domingue was exporting half of the world’s coffee and as much sugar as Jamaica, Cuba, and Brazil combined.1 Occupying the western half of the Caribbean island of Hispaniola...

  14. Herpetologists' League Skull Characters and the Cladistic Relationships of the Hispaniolan Dwarf Twig Anolis

    E-Print Network [OSTI]

    Poe, Steven

    to be twig species from Hispaniola (A. darlingtoni), Puerto Rico (A. occultus), and South America (tigrinus to assess the robustness of these clades, but present evidence suggests an invasion of Hispaniola or Puerto to occur on Jamaica (A. val- encienni), Hispaniola (A. fowleri, A. dar- lingtoni, A. sheplani, A. insolitus

  15. International Geology Review, Vol. 47, 2005, p. 233247. Copyright 2005 by V. H. Winston & Son, Inc. All rights reserved.

    E-Print Network [OSTI]

    Abbott Jr., Richard N.

    Hispaniola is one of the four large islands of the Greater Antilles (Fig. 1). Three of the islands (Jamaica, Hispaniola, and Puerto Rico) lie just south of the northern edge of the present-day Carib- bean plate Rico, Hispaniola, and the southe

  16. TROPICAL LEPIDOPTERA, 9(2): 45-53 THREE NEW SPECIES OF CALISTO

    E-Print Network [OSTI]

    Hedges, Blair

    , Dominican Republic, endemism, Hispaniola, Jamaica, Neotropical, taxonomy, West Indies. The island of Hispaniola, with 36 of the 42 known species of Calisto (sensu Smith, Miller and Miller, 1994), is the center in Hispaniola is not an artifact of collecting. However, the known distribution of species within Hispaniola

  17. Systematic Botany (2003), 28(2): pp. 452460 Copyright 2003 by the American Society of Plant Taxonomists

    E-Print Network [OSTI]

    Olmstead, Richard

    that are endemic to the islands of Cuba, Hispaniola, and Puerto Rico in the Greater Antilles. A phylogenetic of the Greater An- tilles, the archipelago composed of the islands of Cuba, Hispaniola, Jamaica, and Puerto Rico comprises two species, one endemic to Hispaniola, and the other endemic to Puerto Rico. None of the species

  18. Assessing the Potential of Using Hydrate Technology to Capture, Store and Transport Gas for the Caribbean Region

    E-Print Network [OSTI]

    Rajnauth, Jerome Joel

    2012-02-14T23:59:59.000Z

    there are infrastructural constraints such as lack of pipelines. The study shows the gas hydrate value chain for transportation of 5 MMscf/d of natural gas from Trinidad to Jamaica. The analysis evaluated the water required for hydrate formation, effect of composition...

  19. 2013, Latin American Public Opinion Project www.AmericasBarometer.org

    E-Print Network [OSTI]

    Bordenstein, Seth

    to be selected based on mutual consent. Both chambers of the Argentine legislature, as well as the legislature Paraguay Colombia Dom. Rep. Jamaica Bolivia Brazil Ecuador Chile Venezuela Nicaragua Honduras Panama;© 2013, Latin American Public Opinion Project www.AmericasBarometer.org A statistical analysis

  20. Antecedent and anthropogenic influences on the Galveston Island shoreface

    E-Print Network [OSTI]

    Pitkewicz, Jennifer Lynn

    2009-05-15T23:59:59.000Z

    Figure 8: Thickness of Holocene sediments offshore of Jamaica Beach.......................... 20 Figure 9: Stratigraphic correlations of cores along transect 3 with percent sand silt and clay profiles... ................................................................................................. 22 Figure 10: Stratigraphic correlations of layers along transect 3, to scale.......................... 23 Figure 11: Stratigraphic correlations of cores along transect 5 with percent sand silt and clay profiles...

  1. Examining causes of poverty in Latin America and Sub-Saharan Africa

    E-Print Network [OSTI]

    Anderson, Garnett Murphy, II

    2009-06-02T23:59:59.000Z

    .3 years. Costa Rica claims the highest life expectancy at 76.5 years and Bolivia has the lowest at 61.5 years. Other countries with a life expectancy greater than 70 years include: Chile (75.2 years), Jamaica (74.4 years), Panama (74.0 years), Venezuela...

  2. 808 nature geoscience | VOL 3 | NOVEMBER 2010 | www.nature.com/naturegeoscience Backstory | focus

    E-Print Network [OSTI]

    Cai, Long

    How did you end up working in Haiti? On 7 January 2010, an unusual e-mail arrived in my office from Jamaica into Haiti. So extending my research to Haiti was a natural fit. I jumped at the opportunity to join the Haiti study. What was the objective of the work? Originally our objectives were basic

  3. CRC handbook of agricultural energy potential of developing countries. Volume I

    SciTech Connect (OSTI)

    Duke, J.A.

    1986-01-01T23:59:59.000Z

    The contents of this book are: Introduction, Argentina, Bangladesh, Benin, Bolivia, Botswana, Bourkina (Upper Volta), Brazil, Burma, Burundi, Cameroon, Chad, Chile, Columbia, Costa Rica, Djibouti, Dominican Republic, Ecuador, El Salvador, Ethiopia, French Guiana, Gambia, Ghana, Guatemala, Guinea, Guyana, Haiti, Honduras, India, Indonesia, Jamaica, Appendix I. Conventional and Energetic Yields, Appendix II, Phytomass Files, and References.

  4. PEER-REVIEWED PUBLICATIONS: Land, L. S., 1964, Eolian cross-bedding in the beach dune environment, Sapelo Island, Georgia.

    E-Print Network [OSTI]

    Yang, Zong-Liang

    , Sedimentation in a meandering estuary. Sedimentology, v. 6, p. 191-207. Land, L. S., 1966, Diagenesis from a fossil water table. Sedimentology, v. 14, p. 175-185. Land, L. S., and Epstein, S., 1970, Late Pleistocene diagenesis and dolomitization, North Jamaica. Sedimentology, v. 14, p. 184-200. Land, L. S

  5. Grid Induction -2nd hands on workshop

    E-Print Network [OSTI]

    Beimel, Amos

    acquainted with the latest versions of LCG infrastructure, Gilda and its Genius portal. Come and learn how in Spain IHEP in Russia II-SAS in Slovakia IMPB RAS in Russia INFN in Italy INTA in Spain ITEP in Russia JINR in Russia JSI in Slovenia KFKI RMKI in Hungary KIAM RAS in Russia KTH in Sweden KU

  6. Adv. Geosci., 8, 3338, 2006 www.adv-geosci.net/8/33/2006/

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    under a Creative Commons License. Advances in Geosciences Web portal on environmental sciences "ATMOS" E., Tomsk, 634055 Russia 2Institute for Numerical Mathematics RAS, 8 Gubkina Str., Moscow, 119991 Russia 3Institute of Atmospheric Optics SB RAS, 1 Akademicheskii Ave.,Tomsk, 634055 Russia Received: 15 September

  7. Influence of interlayer interaction on tunnelling disordered two-dimensional electron systems

    E-Print Network [OSTI]

    Fominov, Yakov

    systems Institute of Microelectronics Technology RAS, Chernogolovka, Russia #12;IMT RASIMT RAS Yu.V. Dubrovskii E.E. Vdovin L. Eaves P.C. Main O.N. Makarovskii M. Henini V.A.Volkov J.-C. Portal G. Hill #12

  8. The Dictyostelium Mitogen-activated Protein Kinase ERK2

    E-Print Network [OSTI]

    Devreotes, Peter

    The Dictyostelium Mitogen- activated Protein Kinase ERK2 Is Regulated by Ras and cAMP- dependent kinase ERK2 activity (1). In this study we show that other pathways required for aggregation, including Ras and cAMP-dependent protein kinase (PKA), are impor- tant regulators of ERK2 activation

  9. Quaternary Science Reviews 24 (2005) 533569 New insights into the Weichselian environment and climate of the

    E-Print Network [OSTI]

    Ingólfsson, �lafur

    . The Bykovsky record shows how climate change, and the Last Glacial Maximum in particular, affected terrestrial and climate of the East Siberian Arctic, derived from fossil insects, plants, and mammals$ A.V. Shera,Ã?, S Academy of Sciences (RAS), 33 Leninskiy Prospect, 119071 Moscow, Russia b Paleontological Institute, RAS

  10. Feb. 11, 2008 Advanced Fault Tolerance Solutions for High Performance Computing 1/47 Advanced Fault Tolerance Solutions

    E-Print Network [OSTI]

    Engelmann, Christian

    Feb. 11, 2008 Advanced Fault Tolerance Solutions for High Performance Computing 1/47 RAS RAS Advanced Fault Tolerance Solutions for High Performance Computing Christian Engelmann Oak Ridge National Solutions for High Performance Computing 2/47 · Nation's largest energy laboratory · Nation's largest

  11. 1/30/2004 1 Reliable Return Address Stack

    E-Print Network [OSTI]

    Kaeli, David R.

    or if the function entry address is not available yet 1/30/2004 8 A R entry return RAS O entry exit APT Program Check for direct recursion Push onto RAS APT table: Record entry/exit address pairs of called entries ­ Multithreading ­ Speculative execution ­ Non-LIFO function calls 1/30/2004 7 Solution: Reliable

  12. Liaison activities with the Institute of Physcial Chemistry of the Russian Academy of Sciences: Midyear report

    SciTech Connect (OSTI)

    Delegard, C.H.

    1996-05-29T23:59:59.000Z

    The task `IPC/RAS Liaison and Tank Waste Testing` is a program being conducted in fiscal year (FY) 1996 with the support of the U.S. Department of Energy (DOE) Office of Science and Technology, EM-53 Efficient Separations and Processing (ESP) Crosscutting Program, under the technical task plan (TTP) RLA6C342. The principal investigator is Cal Delegard of the Westinghouse Hanford Company. The task involves a technical liaison with the Institute of Physical Chemistry of the Russian Academy of Sciences (IPC/RAS) and their DOE-supported investigations into the fundamental and applied chemistry of the transuranium elements (primarily neptunium, plutonium, and americium) and technetium in @ine media. The task has three purposes: 1. Providing technical information and technical direction to the IPC/RAS. 2. Disseminating IPC/RAS data and information to the DOE technical community. 3. Verifying IPC/RAS results through laboratory testing and comparison with published data.

  13. Abstract: In this paper, we propose a novel method for tuning the amount of generation tripping that would be used in conjunction with remedial action schemes (RAS) or special protection schemes. The method is motivated by the concept of unstable limit cy

    E-Print Network [OSTI]

    Venkatasubramanian, Mani V.

    schemes, power system stability, dynamic security assessment, unstable limit cycles, eigenvalues, power system dynamics. I. Introduction Dynamic security characterizes the ability of a power system to survive usually restrict the tie-line interface power flows, the total generation output for certain plants

  14. The (Re)Construction of Realities in Systems Biology The Jacobian Way

    E-Print Network [OSTI]

    Rostock, Universität

    P MEK P ERK P P ERK P P P P Gene Elk SAP SUR-8SUR-8 KsrKsr MP1MP1 Hsp90Hsp90 14-3-3 ReceptorReceptor Growth factors receptor Ras kinase Raf P P P P MEK P ERK P P Growth factors receptor RasRas kinase Raf P P P P MEK P ERK P P Raf P P P P MEK P ERK P P ERK P P P P ERK P P ERK P P PP PP Gene Elk SAP Gene

  15. Relation restricted prediction analysis Ivo Dntsch

    E-Print Network [OSTI]

    Düntsch, Ivo

    an ordinal attribute" situation ((n, o)-prediction), as well as in the (o, o)-situation. We show how 61 14 25 (6) Thailand 2.1 8 142 20 36 (7) Colombia 2.7 47 284 16 37 (8) Malaysia 1.6 29 313 18 38 (9 Indonesia 0 0 0 1 25 Thailand 1 0 0 1 36 Colombia 1 2 1 1 37 Malaysia 0 1 2 1 38 Guayana 2 1 2 0 42 Jamaica

  16. Supplement 22, Part 6, Section B. Subject Headings: J-Z, Parasite-Subject Catalogue, Subject Headings and Treatment

    E-Print Network [OSTI]

    Zidar, Judith A.; Shaw, Judith H.; Hanfman, Deborah T.; Kirby, Margie D.; Rayburn, Jane D.; Edwards, Shirley J.; Hood, Martha W.

    1979-01-01T23:59:59.000Z

    , H. ?.; and Baumhover, A. H., 1977, Southwest. Entom., v. 2 (4), 202- 206 Cochliomyia hominivorax, serious pest of live stock, feasibility of eradication by sterile insect method: Jamaica Japan Kagei, N.; Kihata, M.; and Hirayama, T., 1975... involved Kidney Huizinga, H. W.; Cosgrove, G. E.; and Sturrock, R. F., 1976, J. Wildlife Dis., v. 12 (1), 93-96 Capillaria sp. in Herpestes auropunctatus, kidney pathology: St. Lucia, West Indies Kidney Hussein, H. S., 1977, J. Comp. Path., v. 87 (2...

  17. South America, Central America, the Caribbean, and Mexico

    SciTech Connect (OSTI)

    Deal, C.

    1981-10-01T23:59:59.000Z

    Summaries of oil and gas drillings, well completions, production, exploratory wells, exploration activity and wildcat drilling were given for South America, Central America, the Caribbean, and Mexico. The countries, islands, etc. included Argentina, Bahamas, Barbados, Belize, Bolivia, Brazil, Colombia, Costa Rica, Cuba, Dominican Republic, Ecuador, French Guiana, Guatemala, Guyana, Haiti, Honduras, Jamaica, Leeward and Windward Islands, Mexico, Netherlands Antilles, Nicaragua, Panama, Paraguay, Peru, Puerto Rico, El Salvador, Surinam, Trinidad and Venezuela. 16 figures, 120 tables. (DP)

  18. Surface Plasmon Resonance Imaging Measurements of Electrostatic Biopolymer

    E-Print Network [OSTI]

    Surface Plasmon Resonance Imaging Measurements of Electrostatic Biopolymer Adsorption onto surface plasmon resonance (SPR) imaging experiments is used to charac- terize the differential/RAS) and surface plasmon resonance (SPR) thickness mea- surements. A schematic diagram of the scanning SPR

  19. Coordinated regulation by Shp2 tyrosine phosphatase of multiple signals controlling insulin biosynthesis in pancreatic beta-cells

    E-Print Network [OSTI]

    Zhang, Sha

    2008-01-01T23:59:59.000Z

    mechanisms of the Helicobacter pylori CagA protein. Nat RevY. , et al. (2004). Helicobacter pylori CagA induces Ras-an intracellular target of Helicobacter pylori CagA protein.

  20. Altered renal function and the development of angiotensin II-dependent hypertension 

    E-Print Network [OSTI]

    Ashek, Ali

    2011-07-05T23:59:59.000Z

    Inappropriate modulation of the renin angiotensin system (RAS) can lead to derangements of blood pressure homeostasis in humans. Cyp1a1-mRen2.F transgenic rats were used to define the renal mechanisms underlying the ...

  1. adolf konrad stips: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    www.maa.orgsitesdefaultfilespdfupload-library22FordThiele1-24.pd 12;Hilbert's 10th problem http:logic.pdmi.ras.ruHilbert10statstat.html D Waldschmidt, Michel First...

  2. USAID Regional Integrated Pest Management (IPM) Collaborative Research Support (CRSP)

    E-Print Network [OSTI]

    Biolaboratory, Kyrgyzstan - Advisory Training Center of Rural Advisory Services (ATC-RAS), Kyrgyzstan - Agricultural Extension Training Center (TES), Osh, Kyrgyzstan - Tacis/SITAF, Kulob, Kyrgyzstan - International centers (IARCs), and NGOs from Tajikistan, Uzbekistan Kyrgyzstan and Kazakhstan attended this Forum

  3. Identification of conserved chromatin-regulatory complexes among the class B synthetic multivulva proteins

    E-Print Network [OSTI]

    Harrison, Melissa M

    2006-01-01T23:59:59.000Z

    The class A, B, and C synthetic Multivulva (synMuv) genes act redundantly to antagonize Ras-mediated vulval induction in C. elegans. Many of these genes encode proteins that are likely to function in transcriptional ...

  4. Supporting Information Detailed Structure of the H2PO4

    E-Print Network [OSTI]

    Gerwert, Klaus

    attacking water being necessary for hydrolysis. In total 28 atoms were described by the SCC with the CHARMM force field.12 The system for the substate GTP in the Ras·GAP was firstly pre-equilibrated for 500

  5. Journal of Bioinformatics and Computational Biology Vol. 4, No. 2 (2006) 589596

    E-Print Network [OSTI]

    Pervouchine, Dmitri D.

    . DANILOVA Institute for Problems of Information Transition RAS Bolshoi Karetnyi per. 19, Moscow, 127994 folding. 589 #12;590 L. V. Danilova et al. 1. Introduction RNA secondary structure prediction is a classic

  6. Parallel Web Scripting with Reactive Constraints Thibaud Hottelier

    E-Print Network [OSTI]

    Bodik, Rastisla

    Parallel Web Scripting with Reactive Constraints Thibaud Hottelier James Ide Doug Kimelman Ras Bodik Electrical Engineering and Computer Sciences University of California at Berkeley Technical Report to lists, requires prior specific permission. #12;Parallel Web Scripting with Reactive Constraints Thibaud

  7. Feasibility of Wholesale Electricity Competition in a Developing Country: Insights from Simulating a Market in Maharashtra State, India

    E-Print Network [OSTI]

    Phadke, Amol

    2007-01-01T23:59:59.000Z

    India looking at 2 mt more LNG from RasGas”. August 29 thhas planned for major LNG imports. Minimum flow constraintsbasis. Liquefied Natural Gas (LNG) Prices India is currently

  8. FREE ELECTRON LASER FOR SIBERIAN CENTRE FOR PHOTOCHEMICAL RESEARCH: THE CONTROL SYSTEM FOR THE

    E-Print Network [OSTI]

    Kozak, Victor R.

    FREE ELECTRON LASER FOR SIBERIAN CENTRE FOR PHOTOCHEMICAL RESEARCH: THE CONTROL SYSTEM of the Siberian Branch of RAS. Abstract. A control system for the magnetic system of the free electron laser (FEL

  9. asteroid watch fireballs: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    at early times and softer later on. The results are applicable to models of gamma-ray bursts. Submitted to Monthly Notices of the RAS Figures available on request from the...

  10. A new mouse model to explore the initiation, progression, and therapy of BRAF(V600E)-induced lung tumors

    E-Print Network [OSTI]

    Dankort, David; Filenova, Elena; Collado, Manuel; Serrano, Manuel; Jones, Kirk; McMahon, Martin

    2007-01-01T23:59:59.000Z

    and RAS mutations in human lung cancer and melanoma. Cancerreceptor signaling pathway in lung cancers. Int. J. Can- ceractivated in non-small-cell lung cancer and associated with

  11. In vitro and in vivo effects of geranylgeranyltransferase I inhibitor P61A6 on non-small cell lung cancer cells

    E-Print Network [OSTI]

    2013-01-01T23:59:59.000Z

    P61A6 on non-small cell lung cancer cells. BMC Cancer 2013in mice with K-RAS-induced lung cancer. J Clin Invest 2007,advances in non-small cell lung cancer biology and clinical

  12. Anti-Tumor Effect in Human Lung Cancer by a Combination Treatment of Novel Histone Deacetylase Inhibitors: SL142 or SL325 and Retinoic Acids

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    of a K-ras ribozyme against human lung cancer cell lineHeymach JV, Lippman SM (2008) Lung cancer. N Engl J Med 359:for advanced non-small cell lung cancer. J Thorac Oncol 2:

  13. Information Systems 28 (2003) 241242 Special issue on data management in bioinformatics

    E-Print Network [OSTI]

    Zaki, Mohammed Javeed

    , discusses the application of the array DBMS, RasDaMan, to the management of gene expression data in situ biological data, namely, the inherent complexity of biological data, domain knowledge barrier, the evolution

  14. CBE Portable Wireless Monitoring System (PWMS): UFAD Systems Commissioning Cart Design Specifications and Operating Manual

    E-Print Network [OSTI]

    Webster, Tom; Bauman, Fred; Anwar, G.

    2012-01-01T23:59:59.000Z

    mote readings. Figure 17: PLM motes default view 5. When theTest Type ? SS, SZ, MZ, PLM Value/Definitions check boxes,complete Click on RAS or PLM Trend tabs Logging complete

  15. UFAD Commissioning Cart: Design Specifications and Operating Manual

    E-Print Network [OSTI]

    Webster, Tom; Arens, Edward; Anwar, George; Bonnell, J.; Bauman, Fred; Brown, C.

    2007-01-01T23:59:59.000Z

    mote readings. Figure 17: PLM motes default view 5. When theTest Type • SS, SZ, MZ, PLM Value/Definitions check boxes,complete Click on RAS or PLM Trend tabs Logging complete

  16. Office for Residential Life & Housing Services University of Rochester

    E-Print Network [OSTI]

    Cantlon, Jessica F.

    Office for Residential Life & Housing Services University of Rochester RESIDENT ADVISOR POSITION DESCRIPTION Resident Advisors help build healthy and inclusive residential communities that complement and extend classroom learning. RAs are expected to create intellectually active residential environments

  17. HER receptor-mediated dynamic signalling in breast cancer cells 

    E-Print Network [OSTI]

    Hu, Huizhong

    2011-07-05T23:59:59.000Z

    The dynamics of cell signalling are critical to cell fate decisions. Human Epidermal growth factor Receptors (HERs)-mediated Ras/Raf/MEK/ERK and PI3K/Akt signalling cascades relay extracellular signals from the plasma ...

  18. Liaison activities with the institute of physical chemistry, Russian academy of sciences: FY 1996

    SciTech Connect (OSTI)

    Delegard, C.H.

    1996-09-23T23:59:59.000Z

    The task ``IPC/RAS Liaison and Tank Waste Testing`` is a program being conducted in fiscal year (FY) 1996 with the support of the U.S. Department of Energy (DOE) Office of Science and Technology, EM-53 Efficient Separations and Processing (ESP) Crosscutting Program, under the technical task plan RLA6C342. The principal investigator is Cal Delegard of the Westinghouse Hanford Company. The task involves a technical liaison with the Institute of Physical Chemistry of the Russian Academy of Sciences (IPC/RAS) and their DOE-supported investigations into the fundamental and applied chemistry of the transuranium elements (primarily neptunium, plutonium, and americium) and technetium in alkaline media. The task has three purposes: 1. Providing technical information and technical direction to the IPC/RAS. 2. Disseminating IPC/RAS data and information to the DOE technical community. 3. Verifying IPC/RAS results through laboratory testing and comparison with published data. This report fulfills the milestone ``Provide End-of-Year Report to Focus Area,`` due September 30, 1996.

  19. Spiral CT Quantification of Aorto-Renal Calcification and Its Use in the Detection of Atheromatous Renal Artery Stenosis: A Study in 42 Patients

    SciTech Connect (OSTI)

    Gayard, Pierre; Garcier, Jean-Marc; Boire, Jean-Yves; Ravel, Anne; Perez, Nessim; Privat, Christian; Lucien, Pascal; Viallet, Jean-Francois; Boyer, Louis [Department of Radiology, University Hospital, BP 69, F-63003 Clermont Ferrand (France)

    2000-01-15T23:59:59.000Z

    Purpose: To investigate whether a correlation exists between aortic and renal arterial calcifications detected with spiral CT and significant angiographic renal artery stenosis (RAS).Methods: Forty-two patients (mean age 67 years, range 37-84 years), of whom 24 were hypertensive, prospectively underwent abdominal helical CT and aortic and renal arteriography. The 3-mm thickness CT scans (pitch = 1) were reconstructed each millimeter. A manual outline of the renal artery including its ostial portion was produced. Calcific hyperdensities were defined as areas of density more than 130 HU. CT data were compared with the presence or absence of RAS on angiography (24 cases); hypertension and age were taken into account (Mann-Whitney U-test).Results: CT detection and quantification appeared to be reliable and reproductible. We did not find any correlation between aortic and renal arterial calcifications and RAS, even for the patients above 65 years, with or without hypertension. There was no correlation either between calcifications and hypertension in patients without RAS. Conclusion: In this population, aortic and renal arterial calcifications have no predictive value for RAS.

  20. Water quality of some coastal canal communities in the Galveston County area

    E-Print Network [OSTI]

    Hall, Ernest Robert

    1976-01-01T23:59:59.000Z

    FOUND IN Peae THE CANAL SYSTFMS. 42. COh'IPARISON OF THF. AVFRAGE TOTAL ORGANIC CARBON (TOC) VALUES FOUND IN THE CANAL SYSTFMS. 43. COMPARISON OF THF AVFRAhE BIOCHFhlICAL OXYGEN DFh'lAND (GODS) VALUES FOU"JD IN THE CANAL SYSTFM. 44. COMPARISON... SAMPLES IV. SEDIEMENT VALUES FOUI'ID IN THF CANAL COMMUNITIES. . . V. COMPARISON OF OXYGEN DEMAND AND UPTAKE RATES BETi!EEN DIFFERENT SFCTIONS OF BAYOU VISTA AND JAMAICA BEACH. VI. COMPARISON OF K RATES AND TOTAL OXYGEN DFMAh!D BETNEEN DIFFERENT...

  1. A shipping crate from the 1865 California shipwreck Brother Jonathan: hardware from the Russell and Erwin Manufacturing Company 

    E-Print Network [OSTI]

    Sowden, Carrie Elizabeth

    2006-08-16T23:59:59.000Z

    in either New York or New Orleans. Despite the name ?Express,? the trip to Chagres was not as fast as it implied. Before 1852, many of the steamers leaving from New York would make multiple stops prior to their arrival in Panama and Nicaragua...; these ports included the southern towns of Charleston, South Carolina, Savannah, Georgia, and Havana, Cuba (Kemble 1990:146). With every trip, even after 1852, steamers stopped at Kingston, Jamaica to refuel; this is where the Brother Jonathan took on more...

  2. Investigation of radiation patterns in the 8-12 micron range as observed by Tiros III over the Caribbean Sea during the period 20-21 July 1961

    E-Print Network [OSTI]

    Mohler, Paul Irvin

    1963-01-01T23:59:59.000Z

    low pressure area in the general vicinity of Panama and western Columbia. It is also known from a study by Cramer (1963) that an upper-level trough existed from Costa Rica to Jamaica. The main features shown by Figure 2 are the very close... relationship between the radiation cold center and the heavy cloud cover around the Panama area and the warm radiation tongue and absence of clouds in the central Caribbean Sea. There also appeared to be a very good re- lationship between the warm radiation...

  3. Invasive potential of common carp (*Cyprinus carpio*) and Nile tilapia (*Oreochromis niloticus*) in American freshwater systems

    E-Print Network [OSTI]

    Zambrano, Luis; Martí nez-Meyer, Enrique; Menezes, Naercio; Peterson, A. Townsend

    2006-07-25T23:59:59.000Z

    from the US Geological Survey’s Hydro-1K data set; http:// edcdaac.usgs.gov/gtopo30/hydro). We also used aspects of climate, including 1961–1990 annual means representing di- urnal temperature range; freeze days; mean annual precipita- tion; solar..., and University of Florida. References Aiken, K.A., Morris, D., Hanley, F.C., and Manning, R. 2002. Aquaculture in Jamaica. Naga, 25: 10–15. Alceste, C.C., Illingworth, J.A., and Jory, D.E. 2001. Tilapia farm- ing industry in Ecuador. Aquac. Mag. 27: 77...

  4. Energy Department Sends First Energy Saving Team to Federal Government Site

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube|6721 Federal RegisterHydrogen and FuelDefense asDepartmentandPaducahin Jamaica,

  5. James City County, Virginia: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica: Energy

  6. James Monroe Capital Corporation | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica: EnergyMonroe

  7. Jamesburg, New Jersey: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica:

  8. Jamesport, New York: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica:Jamesport, New

  9. Jamestown Board of Public Util | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica:Jamesport,

  10. Jamestown, Indiana: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergyKansas:SouthOhio:Jamaica:Jamesport,Indiana:

  11. Ionospheric effects during first 2 hours after the Chelyabinsk meteorite impact

    E-Print Network [OSTI]

    Berngardt, O I; Zherebtsov, G A; Kusonski, O A; Grigorieva, S A

    2013-01-01T23:59:59.000Z

    This paper presents the analysis of ionospheric effects in the region close to the Chelyabinsk meteorite explosion at 03:20UT 2013 February 15 from the Institute of Solar-Terrestrial Physics of Siberian Branch of Russian Academy of Sciences (ISTP SB RAS) EKB radar data, and from the Institute of Geophysics of Ural Branch of Russian Academy of Sciences (IG UB RAS) PARUS ionosonde data. Both instruments are located within the IG UB RAS Arti Observatory approximately 200 km northward from the estimated explosion site. According to the data obtained, the ionospheric disturbance caused by the meteorite flyby, explosion, and impact had high dynamics and amplitude. However, it obviously did not lead to a variation in the ionosphere mean parameters in the region above the disturbance center during the first 2 hours. Essential effects, however, were observed at more than 100-200 km from the explosion site and farther up to 1500 km.

  12. Real-time monitoring of P-based semiconductor growth by linear-optical spectroscopy

    SciTech Connect (OSTI)

    Knorr, K.; Rumberg, A.; Zorn, M.; Meyne, C.; Trepk, T.; Zettler, J.T.; Richter, W. [Technischen Univ., Berlin (Germany). Inst. fuer Festkoerperphysik; Kurpas, P.; Weyers, M. [Ferdinand-Braun-Inst. fuer Hoechstfrequenztechnik, Berlin (Germany)

    1996-12-31T23:59:59.000Z

    In a metal organic vapor phase epitaxy (MOVPE) reactor two optical methods, reflectance anisotropy spectroscopy (RAS) and spectroscopic ellipsometry (SE) are applied simultaneously for a general characterization of all steps of growth. The deoxidation behavior of InP is studied in order to determine the desorption temperature of InP oxide. The influences of doping to the optical spectra are measured with desorption temperature of InP oxide. The influences of doping to the optical spectra are measured with RAS and the surface temperature of InP is determined in-situ from the optical data. Finally, the growth rates of InGaP on GaAs are determined with RAS by the evaluation of Fabry-Perot like oscillations during growth.

  13. Confirmation of intrinsic electron gap states at nonpolar GaN(1-100) surfaces combining photoelectron and surface optical spectroscopy

    SciTech Connect (OSTI)

    Himmerlich, M., E-mail: marcel.himmerlich@tu-ilmenau.de; Eisenhardt, A.; Shokhovets, S.; Krischok, S. [Institut für Physik and Institut für Mikro- und Nanotechnologien, TU Ilmenau, PF 100565, 98684 Ilmenau (Germany); Räthel, J.; Speiser, E.; Neumann, M. D.; Navarro-Quezada, A.; Esser, N. [Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Albert-Einstein-Strasse 9, 12489 Berlin (Germany)

    2014-04-28T23:59:59.000Z

    The electronic structure of GaN(1–100) surfaces is investigated in-situ by photoelectron spectroscopy (PES) and reflection anisotropy spectroscopy (RAS). Occupied surface states 3.1?eV below the Fermi energy are observed by PES, accompanied by surface optical transitions found in RAS around 3.3?eV, i.e., below the bulk band gap. These results indicate that the GaN(1–100) surface band gap is smaller than the bulk one due to the existence of intra-gap states, in agreement with density functional theory calculations. Furthermore, the experiments demonstrate that RAS can be applied for optical surface studies of anisotropic crystals.

  14. Blognoon: Exploring a Topic in the Blogosphere Maria Grineva

    E-Print Network [OSTI]

    Tomkins, Andrew

    Programming of RAS. Copyright is held by the International World Wide Web Conference Com- mittee (IW3C2. The technology behind Blognoon leverages Wikipedia as the world biggest resource of human knowledge that contain Clean tech concept and also relevant concepts such as Renewable energy and Biofuel. Clean tech

  15. RASRAFMEK-dependent oxidative cell death involving voltage-dependent anion channels

    E-Print Network [OSTI]

    Stockwell, Brent R.

    LETTERS RAS­RAF­MEK-dependent oxidative cell death involving voltage-dependent anion channels J. Fridman1 , Adam J. Wolpaw1 , Inese Smukste1 , John M. Peltier3 , J. Jay Boniface3 , Richard Smith that discriminate between the genetic makeup of normal cells and tumour cells are valuable for treating and under

  16. Overarching Hypothesis of Antarctic RIMEOverarching Hypothesis of Antarctic RIME ""The Ross Sea region is critical in the exchange ofThe Ross Sea region is critical in the exchange of

    E-Print Network [OSTI]

    Howat, Ian M.

    the complexity of these processes in the vicinity of Ross Island. The boundary layer wind, which is influenced by the RAS, is strongly modified by the topography, as is the precipitation distribution. The current generation of atmospheric models gives an incomplete depiction of the profiles of momentum and moisture near

  17. Matthew K. MacDonald 401 Crew Building, Kings Buildings

    E-Print Network [OSTI]

    : hydrometeorology, snow hydrology, land surface modelling, micrometeorology Education: Ph.D. School of Geo-RAS, MESH Other software: R, MATLAB, ArcGIS, Loggernet Instrumentation: Campbell Scientific meteorologicalDonald MK, DeBeer CM, Brown T. 2013. Multi-variable evaluation of hydrological model predictions

  18. Elevator Pitches University of Birmingham

    E-Print Network [OSTI]

    Birmingham, University of

    , Cyber Security, Telecoms, Unified Messaging, CRM (ACT), Storage Solutions, Disaster Recovery, Document;#12;#12;Thank You! Next Pitch #12;Software Tools to Program large distributed infrastructures such as Cloud, Cloud, security What we do - 9 PhD students and RAs -Worked at HP, BT and IBM Resulted in - software

  19. The Sensitivity of Intraseasonal Variability in the NCAR CCM3 to Changes in Convective Parameterization

    E-Print Network [OSTI]

    Maloney, Eric

    The Sensitivity of Intraseasonal Variability in the NCAR CCM3 to Changes in Convective@atmos.washington.edu. #12;1 Abstract The NCAR CCM3.6 simulation of tropical intraseasonal variability in zonal winds and pre- cipitation can be improved by implementing the McRAS convection scheme of Sud and Walker. The default CCM3

  20. Pilot Scale Study of Excess Sludge Production Reduction in Wastewater Treatment by Ozone

    E-Print Network [OSTI]

    Barthelat, Francois

    Pilot Scale Study of Excess Sludge Production Reduction in Wastewater Treatment by Ozone Yuan Ma-scale reactors were operated at the LaPrairie Wastewater Treatment plant (one control and one ozonated) to investigate the sludge reduction potential of partially ozonating sludge return activated sludge (RAS

  1. Controlling the Pecan Nut Casebearer (Spanish)

    E-Print Network [OSTI]

    Knutson, Allen E.; Ree, Bill

    2001-04-11T23:59:59.000Z

    , tierras bajas cercanas a r?os, o tier- ras altas. ? Separe las trampas por una distancia de, por lo menos, varios ?rboles. Coloque las trampas cerca de la terminal de una rama cargada de frutos y a una altura que resulte pr?ctica. Las trampas que se...

  2. FOB Undergoing Test and Alignment in FAS at Ball Aerospace OPTICAL TESTS AND ALIGNMENTS

    E-Print Network [OSTI]

    Schneider, Glenn

    FOB Undergoing Test and Alignment in FAS at Ball Aerospace OPTICAL TESTS AND ALIGNMENTS After the FOB alignment is complete, and it passes environmental tests, it will be checked out in a second HST. The NICMOS FOB will relay simulated images from RAS/HOMS to a Photometrics CCD camera placed where the three

  3. Directory of Integrated Pest Specialists and Stakeholders in

    E-Print Network [OSTI]

    , Transforming Lives #12;KYRGYZSTAN Murat Aitmatov Advisory Training Centre (ATC) of Rural Advisory Services (RAS) 720022, Bishkek, ax: 996-312-682027 43/1 Grajdanskaya St., Kyrgyzstan F E-mail: atc@atc.org.kg Barakanova K Phone: 996-312-647638 Bekboeva Rosa, Ph.D Agrarian Un Bishkek, St., Suerkulova- Kyrgyzstan W Home

  4. Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process

    E-Print Network [OSTI]

    Gilmore, Stephen

    Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process algebra) on the Extracellular signal Regulated Kinase (ERK) signalling pathway [1] through modelling in a Markovian process choices. The system which we consider is the Ras/Raf-1/MEK/ERK signalling path- way, as presented in [1

  5. Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process

    E-Print Network [OSTI]

    Calder, Muffy

    Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process algebra Regulated Kinase (ERK) signalling pathway [5] through modelling in a Markovian process algebra, PEPA [11 durations and probabilistic choices. The system which we consider is the Ras/Raf-1/MEK/ERK signalling

  6. BioCONCUR 2004 Preliminary Version Modelling the influence of RKIP on the ERK

    E-Print Network [OSTI]

    Calder, Muffy

    Bio­CONCUR 2004 Preliminary Version Modelling the influence of RKIP on the ERK signalling pathway Protein (RKIP) on the Extracellular signal Regulated Kinase (ERK) signalling pathway [1] through modelling stochastic durations and probabilistic choices. The system which we consider is the Ras/Raf­1/MEK/ERK

  7. Multiple ERK substrates execute single biological processes in Caenorhabditis elegans

    E-Print Network [OSTI]

    Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ, 2008) RAS-extracellular signal regulated kinase (ERK) signaling governs multiple aspects of cell fate. Understanding how perturbations to the ERK signaling pathway lead to developmental disorders and cancer hinges

  8. Spred2 is involved in imatinib-induced cytotoxicity in chronic myeloid leukemia cells

    SciTech Connect (OSTI)

    Liu, Xiao-Yun; Yang, Yue-Feng; Wu, Chu-Tse; Xiao, Feng-Jun; Zhang, Qun-Wei [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Ma, Xiao-Ni [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Li, Qing-Fang; Yan, Jun [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Wang, Hua, E-mail: wanghualjh@gmail.com [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Wang, Li-Sheng, E-mail: wangls@nic.bmi.ac.cn [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)

    2010-03-19T23:59:59.000Z

    Spreds, a recently established class of negative regulators of the Ras-ERK (extracellular signal-regulated kinase) pathway, are involved in hematogenesises, allergic disorders and tumourigenesis. However, their role in hematologic neoplasms is largely unknown. Possible effects of Spreds on other signal pathways closely related to Ras-ERK have been poorly investigated. In this study, we investigated the in vitro effects of Spred2 on chronic myeloid leukemia (CML) cells. In addition to inhibiting the well-established Ras-ERK cascade, adenovirus-mediated Spred2 over-expression inhibits constitutive and stem cell factor (SCF)-stimulated sphingosine kinase-1 (SPHK1) and Mcl-1 expression, as well as inhibiting proliferation and inducing apoptosis in CML cells. In K562 cells and primary CML cells, imatinib induces endogenous Spred2 expression. Spred2 silencing by stable RNA interference partly protects K562 cells against imatinib-induced apoptosis. Together, these data implicate Spred2 in imatinib-induced cytotoxicity in CML cells, possibly by inhibiting the Ras-ERK cascade and the pro-survival signaling molecules SPHK1 and Mcl-1. These findings reveal potential targets for selective therapy of CML.

  9. Role of Protein Kinase C in the Signal Pathways That Link Na /K -ATPase to ERK1/2*

    E-Print Network [OSTI]

    Askari, Amir

    Role of Protein Kinase C in the Signal Pathways That Link Na /K -ATPase to ERK1/2* Received, transactivation of EGFR, and activation of the Ras/ ERK1/2 cascade. To determine the role of protein kinase C (PKC, but not toxic, effects of ouabain in rat cardiac ventricles. Ouabain-induced activation of ERK1/2 was blocked

  10. Developmental Cell MPK-1 ERK Controls Membrane

    E-Print Network [OSTI]

    Developmental Cell Article MPK-1 ERK Controls Membrane Organization in C. elegans Oogenesis via/ERK in the proximal germ- line to control plasma membrane biogenesis and organization during oogenesis and oocyte production is dramatically downregulated. The RTK-RAS-ERK pathway relays physiological and devel

  11. Modelling the Influence of RKIP on the ERK Signalling Pathway Using the Stochastic Process

    E-Print Network [OSTI]

    Swain, Peter

    Modelling the Influence of RKIP on the ERK Signalling Pathway Using the Stochastic Process Algebra the influence of the Raf Kinase In- hibitor Protein (RKIP) on the Extracellular signal Regulated Kinase (ERK durations and probabilistic choices. The system which we consider is the Ras/Raf-1/MEK/ERK signalling

  12. Super-Elastic and Plastic Shock Waves Generated by Lasers N.A. Inogamov a,1

    E-Print Network [OSTI]

    Fominov, Yakov

    Super-Elastic and Plastic Shock Waves Generated by Lasers N.A. Inogamov a,1 , V.V. Zhakhovsky b,3 fortov@ihed.ras.ru, i oleynik@usf.edu Keywords: Femtosecond laser-matter interactions, elastic-plastic there is an elastic shock wave (SW), which propagates before the strong plastic shock with plastic pressures of up

  13. C16C29 homologous series of monomethylalkanes in the pyrolysis products of a Holocene microbial mat

    E-Print Network [OSTI]

    Kenig, Fabien

    products of kerogen isolated from a Holocene microbial mat collected in Abu Dhabi (United Arab Emirates assemblage sampled from the Abu Dhabi sabkha (United Arab Emirates) are analysed for MMAs. The carbon number was collected in Abu Dhabi (United Arab Emirates) along a section of the Ras al Khaf channel (Kenig, 1991

  14. Preliminary determination of the interdependence among strong-motion amplitude, earthquake magnitude and hypocentral

    E-Print Network [OSTI]

    Parvez, Imtiyaz Ahmed

    Giuliano F. Panza1,3 and Anatoly G. Petukhin4 1 Department of Earth Sciences, University of Trieste, Via E. Weiss 4, 34127 Trieste, Italy. E-mails: parvez@dst.univ.trieste.it; panza@dst.univ.trieste.it 2, SAND Group, Trieste, Italy 4 Kamchatka Experimental and Methodical Seismological Department GS RAS 9

  15. Project Information Form Project Title Evaluation of the Combined Effect of Recycled Asphalt Pavement (RAP),

    E-Print Network [OSTI]

    California at Davis, University of

    Pavement (RAP), Recycled Asphalt Shingles (RAS), and Different Virgin Binder Sources on Performance of Research Project As virgin material sources become increasingly scarce, and the volume of pavement material for pavement construction. The California Department of Transportation (Caltrans) has recently increased

  16. Posgrado en Direccin y Consultora en Nuevas

    E-Print Network [OSTI]

    Yannuzzi Sanchez, Marcelo

    que permite medir el trabajo que deben realizar los alumnos para la adquisi- ción de los conocimientos, capacidades, y competencias necesarias para superar las diferentes materias de su plan de estudios. Cada ECTS equivale a 25 horas de estu- dio e incluye el tiempo dedicado a las ho- ras lectivas, horas de trabajo del

  17. Atmos. Chem. Phys., 14, 69536965, 2014 www.atmos-chem-phys.net/14/6953/2014/

    E-Print Network [OSTI]

    Pierce, Jeffrey

    , as in the case of sand, but with opposite slopes. 1 Introduction Studies of climate change are of enormous. Tzanis1, and A. V. Vasilyev4 1Climate Research Group, Division of Environmental Physics and Meteorology RAS), Russian Academy of Sciences, St. Petersburg, Russia 3Division of Electronic Engineering

  18. Long-Term Climate Change Commitment and Reversibility: An EMIC Intercomparison KIRSTEN ZICKFELD,a

    E-Print Network [OSTI]

    Matsumoto, Katsumi

    Long-Term Climate Change Commitment and Reversibility: An EMIC Intercomparison KIRSTEN ZICKFELD. Obukhov Institute of Atmospheric Physics, RAS, Moscow, Russia f Potsdam Institute for Climate Impact of Intermediate Complexity (EMICs) undertaken in support of the Intergovernmental Panel on Climate Change (IPCC

  19. Hydrol. Earth Syst. Sci., 11, 18311841, 2007 www.hydrol-earth-syst-sci.net/11/1831/2007/

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    System Modeling, Pacific Institute of Geography, FEB RAS, Russia 2Delft University of Technology, Faculty by statistically significant changes in such monthly teleconnection indices, as the Arctic and North Pacific by Thomp- son and Wallace (1998, 2000). Another well known climate decennial-scale phenomenon, named

  20. Recent Climate Changes in Precipitable Water in the Global Tropics as Revealed in NCEP/NCAR Reanalysis

    E-Print Network [OSTI]

    Hawai'i at Manoa, University of

    1 Recent Climate Changes in Precipitable Water in the Global Tropics as Revealed in NCEP: 1 (808) 956-2877 Email: chu@hawaii.edu #12;2 Abstract For the first time, long-term climate changes/NCAR Reanalysis Igor I. Zveryaev and Pao-Shin Chu* P.P. Shirshov Institute of Oceanology, RAS, Moscow, Russia

  1. RELIABILITY, AVAILABILITY, AND SERVICEABILITY FOR PETASCALE HIGH-END COMPUTING AND BEYOND

    SciTech Connect (OSTI)

    Chokchai "Box" Leangsuksun

    2011-05-31T23:59:59.000Z

    Our project is a multi-institutional research effort that adopts interplay of RELIABILITY, AVAILABILITY, and SERVICEABILITY (RAS) aspects for solving resilience issues in highend scientific computing in the next generation of supercomputers. results lie in the following tracks: Failure prediction in a large scale HPC; Investigate reliability issues and mitigation techniques including in GPGPU-based HPC system; HPC resilience runtime & tools.

  2. The Astrophysical Journal, 757:92 (6pp), 2012 September 20 doi:10.1088/0004-637X/757/1/92 C 2012. The American Astronomical Society. All rights reserved. Printed in the U.S.A.

    E-Print Network [OSTI]

    Usoskin, Ilya G.

    . INTRODUCTION Sporadic energy releases on the Sun can accelerate solar ener- getic particles (SEPs. The American Astronomical Society. All rights reserved. Printed in the U.S.A. OCCURRENCE OF EXTREME SOLAR-90014 Oulu, Finland; ilya.usoskin@oulu.fi 2 Ioffe Physical-Technical Institute of RAS, 194021 St

  3. Signal-transducing function of Na -K -ATPase is essential for ouabain's effect on [Ca2

    E-Print Network [OSTI]

    Brand, Paul H.

    of the early signaling events, including the stimulation of protein tyrosine kinases and production of ROS protein kinases (MAPKs), increased production of re- active oxygen species (ROS), and raised both systolic of a dominant negative Ras, antagonized ouabain-induced activation of MAPKs and increases in [Ca2 ]i. Treatment

  4. Cyber-Physical Aspects of Energy Systems for the 21st

    E-Print Network [OSTI]

    Mueller, Frank

    and technology in the electric power and renewable energy industries, providing long-term energy security and also highlight novel opportunities for selective power delivery during power outages via islanding of smart microgrids. islanding RAS distributed network overlay hierarchical control via leader election

  5. Weights of Exact Threshold Functions Laszlo Babai1

    E-Print Network [OSTI]

    Babai, László

    arnsfelt@cs.au.dk 3 Steklov Mathematical Institute podolskii@mi.ras.ru 4 ITCS, Tsinghua University inputs holds. The related class of (linear) threshold functions consist of those Boolean functions that decide whether a real valued linear inequality in their Boolean inputs holds. To be more precise

  6. U.S. Department of the Interior November 2013 U.S. Geological Survey

    E-Print Network [OSTI]

    (t/yr) at its 270,000-t/yr Hannibal smelter. A decision by the Public Utilities Commission of Ohio/yr smelter in Ras al Khair, Saudi Arabia. Potline instability was cited as the reason for the shutdown least efficient smelters in Russia, citing high costs. The Ural, Volgograd, and Volkhov smelters were

  7. DEPARTAMENTO DE GENTICA Y BIOLOGA MOLECULAR

    E-Print Network [OSTI]

    - canismos involucrados en la resistencia a drogas en Giardia duodenalis. Estu- dio sobre el ciclo celular en transcripción de genes eucariónticos. Estudio de oncogenes (myc, ras) y anti-oncogenes (p53, Rb) celulares de investigación: Regulación de la expresión génica y cáncer. Mecanismos de transformación celular

  8. Research article 850 TheJournalofClinicalInvestigation http://www.jci.org Volume 120 Number 3 March 2010

    E-Print Network [OSTI]

    Boyer, Edmond

    Cdisruptionisaputativealternativetargetedtherapeu- ticstrategyforthetreatmentofNB. Introduction According to the classic neurotrophic theory, neuronal survival is dependent March 2010 Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting Trk various mech- anisms generates survival signals via the PI3K/Akt and Ras/MEK/ MAPK pathways, which in turn

  9. New Approaches for Calculating Safety Parameters and Estimating Hazard Severity for Cumene Hydroperoxide

    E-Print Network [OSTI]

    Chen, Shu-Ching

    to Fire and/or Explosion in the Chemical Process Industries [1-4] Year Location Chemical Event Deaths/injured 1943 Ludigshafen, Germany Butadiene Explosion >100d 1944 Cleveland, OH LNG Fire 128/200~400 1947 Texas ?/>200 1962 Ras Taruna, Saudi Arabia Propane Fire 1/111 1964 Tokyo, Japan MEKPO Fire/Explosion 19

  10. A Proposal for a Detector 2 km Away From the T2K Neutrino May 30, 2005

    E-Print Network [OSTI]

    Boyer, Edmond

    energies. High energy muons which exit the water Cherenkov detector will be measured by an iron muon ranger Institute of Experimental Physics, Warsaw University, Warszawa (Poland): D. Kielczewska1 H.Niewodnicza´nski Institute of Nuclear Physics, Krak´ow (Poland): A. Szelc, A. Zalewska Institute for Nuclear Research RAS

  11. On Reaching Consensus by a Group of Collaborating Agents

    E-Print Network [OSTI]

    Ras, Zbigniew W.

    -Japanese Institute of Information Technology, 02-008 Warsaw, Poland 3 Bialystok Tech. Univ., Dept. of Mech. Eng. & Applied Comp. Sci., 15-351 Bialystok, Poland ras@uncc.edu, adardzin@uncc.edu Abstract. In this paper-dimensional model in which the main elements are stress and energy laid out on 2 perpendicular axes. Stress can

  12. On the ac magnetic susceptibility of spin-chains: solitons in one-dimensional systems

    E-Print Network [OSTI]

    Fominov, Yakov

    University, Krakow, Poland E. Pikina Institute for Problems of Oil and Gas RAS, Moscow, Russia W. Haase . What is time * ? * = + T E T B b H exp )( 1 10 ( )TE /exp0 * = E ­ activation energy frequencies. ),()( 0max Tff = increases with the increase in activation energy E, and in ratio a #12;The

  13. Universal Method for Protein Immobilization on Chemically Functionalized Germanium Investigated by ATR-FTIR Difference

    E-Print Network [OSTI]

    Gerwert, Klaus

    of the "on" and "off" switching of Ras at atomic resolution. Furthermore, the activity of immobilized PS I is a widely used label-free technique that detects mass changes at the sensor surface.8 Label-free methods are becoming increasingly popular as the attached label can interfere with the reaction and the labeling

  14. S t a t eEngineeringEngineeringThe Magazine of the Penn State College of Engineering Summer 2001 For more information

    E-Print Network [OSTI]

    Yener, Aylin

    of Engineering Energy Technology Projects office and the College of Education, is intended for K-12 teachers interested in nuclear science and technology. The workshop addresses both the benefits and risks associated Park This advanced program provides experienced users of HEC-RAS software an opportunity to practice

  15. Over the past several years, Lyon Arboretum staff along

    E-Print Network [OSTI]

    and her students for furnishing us with Chinese, Japanese, and Korean language translations, and Ras important to her. I recorded many stories about the early stages in her de- veloping the Ethnobotanical was the first woman in her home state of Arkansas to be accepted in the Peace Corps and was sent to the Philip

  16. Acute ethanol intake induces superoxide anion generation and mitogen-activated protein kinase phosphorylation in rat aorta: A role for angiotensin type 1 receptor

    SciTech Connect (OSTI)

    Yogi, Alvaro; Callera, Glaucia E. [Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, Ontario (Canada)] [Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, Ontario (Canada); Mecawi, André S. [Department of Physiology, Faculty of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP (Brazil)] [Department of Physiology, Faculty of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP (Brazil); Batalhão, Marcelo E.; Carnio, Evelin C. [Department of General and Specialized Nursing, College of Nursing of Ribeirão Preto, USP, São Paulo (Brazil)] [Department of General and Specialized Nursing, College of Nursing of Ribeirão Preto, USP, São Paulo (Brazil); Antunes-Rodrigues, José [Department of Physiology, Faculty of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP (Brazil)] [Department of Physiology, Faculty of Medicine of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP (Brazil); Queiroz, Regina H. [Department of Clinical, Toxicological and Food Science Analysis, Faculty of Pharmaceutical Sciences, USP, São Paulo (Brazil)] [Department of Clinical, Toxicological and Food Science Analysis, Faculty of Pharmaceutical Sciences, USP, São Paulo (Brazil); Touyz, Rhian M. [Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, Ontario (Canada)] [Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, Ontario (Canada); Tirapelli, Carlos R., E-mail: crtirapelli@eerp.usp.br [Department of Psychiatric Nursing and Human Sciences, Laboratory of Pharmacology, College of Nursing of Ribeirão Preto, USP, Ribeirão Preto, SP (Brazil)

    2012-11-01T23:59:59.000Z

    Ethanol intake is associated with increase in blood pressure, through unknown mechanisms. We hypothesized that acute ethanol intake enhances vascular oxidative stress and induces vascular dysfunction through renin–angiotensin system (RAS) activation. Ethanol (1 g/kg; p.o. gavage) effects were assessed within 30 min in male Wistar rats. The transient decrease in blood pressure induced by ethanol was not affected by the previous administration of losartan (10 mg/kg; p.o. gavage), a selective AT{sub 1} receptor antagonist. Acute ethanol intake increased plasma renin activity (PRA), angiotensin converting enzyme (ACE) activity, plasma angiotensin I (ANG I) and angiotensin II (ANG II) levels. Ethanol induced systemic and vascular oxidative stress, evidenced by increased plasma thiobarbituric acid-reacting substances (TBARS) levels, NAD(P)H oxidase?mediated vascular generation of superoxide anion and p47phox translocation (cytosol to membrane). These effects were prevented by losartan. Isolated aortas from ethanol-treated rats displayed increased p38MAPK and SAPK/JNK phosphorylation. Losartan inhibited ethanol-induced increase in the phosphorylation of these kinases. Ethanol intake decreased acetylcholine-induced relaxation and increased phenylephrine-induced contraction in endothelium-intact aortas. Ethanol significantly decreased plasma and aortic nitrate levels. These changes in vascular reactivity and in the end product of endogenous nitric oxide metabolism were not affected by losartan. Our study provides novel evidence that acute ethanol intake stimulates RAS activity and induces vascular oxidative stress and redox-signaling activation through AT{sub 1}-dependent mechanisms. These findings highlight the importance of RAS in acute ethanol-induced oxidative damage. -- Highlights: ? Acute ethanol intake stimulates RAS activity and vascular oxidative stress. ? RAS plays a role in acute ethanol-induced oxidative damage via AT{sub 1} receptor activation. ? Translocation of p47phox and MAPKs phosphorylation are downstream effectors. ? Acute ethanol consumption increases the risk for acute vascular injury.

  17. San Jose Accord: energy aid or petroleum-marketing strategy

    SciTech Connect (OSTI)

    Not Available

    1982-09-30T23:59:59.000Z

    The San Jose Accord was signed in San Jose, Costa Rica on August 3, 1980 by the Presidents of Venezuela and Mexico, whereby the two countries mutually committed to supply the net imported domestic oil consumption of several Central American and Caribbean countries. Countries initially participating in the program are: Barbados, Costa Rica, Dominican Republic, El Salvador, Guatemala, Honduras, Jamaica, Nicaragua, and Panama. Seven eastern Caribbean countries were to meet on October 7 to petition for inclusion in the Accord, namely: Antigua, St. Kitt/Nevis, Montserrat, Dominica, St. Lucia, St. Vincent, and Grenada. The official language of the Accord is presented, and the operative status of the Accord two years after signing is discussed. Specific briefs about some of the individual countries in the Accord are included. The fuel price/tax series for the Western Hemisphere countries is updated.

  18. Waveguide detection of right-angle-scattered light in flow cytometry

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P. (Danville, CA)

    2000-01-01T23:59:59.000Z

    A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.

  19. Oxhide ingots, copper production, and the mediterranean trade in copper and other metals in the bronze age

    E-Print Network [OSTI]

    Jones, Michael Rice

    2007-09-17T23:59:59.000Z

    in ancient times. Although the volume of these slag heaps has been estimated at four million tons, virtually all of the slag deposits in the mining areas appear to date to well after the Bronze Age. 18 While more evidence for Middle and Late Cypriot copper... copper oxhide ingots.......... 105 16 Limestone oxhide ingot mold from Ras Ibn Hani, Syria ............. 106 17 Reconstruction of a Late Bronze Age slag-tapping furnace from Kition, based on archaeological remains...

  20. Notes 14. Experimental identification of bearing force coefficients

    E-Print Network [OSTI]

    San Andres, Luis

    2009-01-01T23:59:59.000Z

    An example of system parameter identification (Hybrid Brush Seal) Luis San Andr?s (lecturer) Thanks to Adolfo Delgado, Jos? Baker (RAs) & support from Siemens Power Generation MEEN 617 - April 2008 Structural parameters K shaft = 243 lbf/in (42...Notes 14. IDENTIFICATION OF BEARING FORCE COEFFICIENTS. ? Dr. Luis San Andr?s (2009) 1 Handout # 14 (MEEN 626) Application example Experimental identification of bearing force coefficients Experimental identification of the dynamic force...

  1. Northern Fur Seals (Callorhinus ursinus) of the Commander Islands: Summer Feeding Trips, Winter Migrations and Interactions with Killer Whales (Orcinus orca) 

    E-Print Network [OSTI]

    Belonovich, Olga Andreevna

    2011-10-21T23:59:59.000Z

    ; and North Pacific Wildlife Consulting LLC, Seattle, USA. Additional funding was provided by Texas A&M University, TX, USA; Kamchatka Branch of the Pacific Geographical Institute, FEB, viii RAS, Petropavlovsk- Kamchatsky, Russia; and Kamchatka Research... Institute of Fisheries and Oceanography, Petropavlovsk-Kamchatsky, Russia. The work was conducted according to permits N 1194 in 2007, N79/2008-H in 2008 and N07-07/3756 in 2009 issued by Russian Federal Agency of Fisheries to Kamchatka Branch...

  2. RAF protein-serine/threonine kinases: Structure and regulation

    SciTech Connect (OSTI)

    Roskoski, Robert, E-mail: rrj@brimr.org [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)] [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)

    2010-08-27T23:59:59.000Z

    Research highlights: {yields} The formation of unique side-to-side RAF dimers is required for full kinase activity. {yields} RAF kinase inhibitors block MEK activation in cells containing oncogenic B-RAF. {yields} RAF kinase inhibitors can lead to the paradoxical increase in RAF kinase activity. -- Abstract: A-RAF, B-RAF, and C-RAF are a family of three protein-serine/threonine kinases that participate in the RAS-RAF-MEK-ERK signal transduction cascade. This cascade participates in the regulation of a large variety of processes including apoptosis, cell cycle progression, differentiation, proliferation, and transformation to the cancerous state. RAS mutations occur in 15-30% of all human cancers, and B-RAF mutations occur in 30-60% of melanomas, 30-50% of thyroid cancers, and 5-20% of colorectal cancers. Activation of the RAF kinases requires their interaction with RAS-GTP along with dephosphorylation and also phosphorylation by SRC family protein-tyrosine kinases and other protein-serine/threonine kinases. The formation of unique side-to-side RAF dimers is required for full kinase activity. RAF kinase inhibitors are effective in blocking MEK1/2 and ERK1/2 activation in cells containing the oncogenic B-RAF Val600Glu activating mutation. RAF kinase inhibitors lead to the paradoxical increase in RAF kinase activity in cells containing wild-type B-RAF and wild-type or activated mutant RAS. C-RAF plays a key role in this paradoxical increase in downstream MEK-ERK activation.

  3. A&A manuscript no. (will be inserted by hand later)

    E-Print Network [OSTI]

    Gehren, Thomas

    are approximately solar above [Fe/H] ;2:2 where they decrease rapidly by 0.5 - 0.6 dex. The direct method based offprint requests to: L. Mashonkina, e-mail: ml@astro.ksu.ras.ru ? Based on observations at the German- citation energy of 0:7 eV) these Ba II lines can be detected even in extremely metal-poor stars. Two types

  4. 4 Science $ervj.ce Feature ? IVHY THE \\iEA'L?;IER

    E-Print Network [OSTI]

    there been uncomfortable and expensive water shortage, but also frlrest fires have been un- Usually the water stored i n the Sier- ras at the end of the winter was the least i n u?ry ;YC"R'~~S. iSot only has juicy oranges have of water this tdnter ! (Tomorrav: Cold Vave Outposts) All rights reserved by Science

  5. Hormonal activation of genes through nongenomic pathways by estrogen and structurally diverse estrogenic compounds 

    E-Print Network [OSTI]

    Li, Xiangrong

    2006-08-16T23:59:59.000Z

    of growth factor receptors. High levels of Erb-B2 and EGFR have been associated with ER-negative breast tumors (Allred et al., 1992; Schroeder et al., 1997; Elledge et al., 1998). Downstream targets of growth factor receptors, such as Ras....1.5.3.5 Trastuzumab..................................... 40 1.2 Estrogen receptor ......................................................................... 40 1.2.1 The structure of estrogen receptor ................................ 40 1.2.2 The physiological...

  6. Percutaneous Embolization of Transhepatic Tracks for Biliary Intervention

    SciTech Connect (OSTI)

    Lyon, Stuart M.; Terhaar, Olaf; Given, Mark F.; O'Dwyer, Helena M.; McGrath, Frank P.; Lee, Michael J. [Beaumont Hospital, Department of Academic Radiology (Ireland)], E-mail: mlee@rcsi.ie

    2006-12-15T23:59:59.000Z

    Significant pain can occur after removing transhepatic catheters from biliary access tracks, after percutaneous biliary drainage (PBD) or stenting. We undertook a randomized prospective study to ascertain whether track embolization decreases the amount of pain or analgesic requirement after PBD. Fifty consecutive patients (M:F, 22:28; age range:29-85 years; mean age: 66.3 years) undergoing PBD were randomized to receive track embolization or no track embolization after removal of biliary drainage catheters. A combination of Lipoidol and n-butyl cyanoacrylate were used to embolize transhepatic tracks using an 8F dilator. The patients who did not have track embolization performed had biliary drainage catheters removed over a guide wire. A visual analog scoring (VAS) system was used to grade pain associated with catheter removal, 24 h afterward. A required analgesic score (RAS) was devised to tabulate the analgesia required. No analgesia had a score of 0, oral or rectal nonopiate analgesics had a score of 1, oral opiates had a score of 2, and parenteral opiates had a score of 3. The average VAS and RAS for both groups were calculated and compared.Seven patients were excluded for various reasons, leaving 43 patients in the study group. Twenty-one patients comprised the embolization group and 22 patients comprised the nonembolization group. The mean biliary catheter dwell time was not significantly different (p > 0.05) between the embolization group and nonembolization (mean: 5.4 days vs 6.9 days, respectively). In the nonembolization group, the mean VAS was 3.4. Eight patients required parenteral opiates, three patients required oral opiates, and five patients required oral or rectal analgesics, yielding a mean RAS of 1.6. In the embolization group, the mean VAS was 0.9. No patient required parenteral opiates, six patients required oral opiates, and two patients had oral analgesia. The average RAS was 0.6. Both the VAS and the RAS were significantly lower in the embolization group compared with the nonembolization group (p < 0.0023 and p < 0.002, respectively). No complications were seen related to track embolization. Percutaneous track embolization after removal of biliary drainage catheters decreases patient's perception of pain and decreases the amount of required analgesia. In particular, the amount of opiate analgesia required is considerably less.

  7. Merging as a key to reforming of disk and AGN triggering in Sy galaxy Mrk334

    E-Print Network [OSTI]

    A. A. Smirnova; A. V. Moiseev

    2008-01-10T23:59:59.000Z

    We have studied the kinematics of the ionized gas and stellar component in Mrk334 using methods of panoramic (3D) spectroscopy. The observations were performed at the prime focus of the SAO RAS 6-m telescope with the integral-field spectrograph MPFS (Afanasiev et al. 2001) and with a scanning Fabry-Perot interferometer (FPI), installed on the multimode device SCORPIO (Afanasiev & Moiseev 2005). Based on these data, the monochromatic maps and velocity fields in different emission lines of the ionized gas were constructed. The diagnostic diagrams have been made based on the emission lines ratios.

  8. Compositions and methods for detecting Noonan syndrome

    DOE Patents [OSTI]

    Gelb, Bruce D. (New York, NY); Tartaglia, Marco (Rome, IT); Pennacchio, Len (Walnut Creek, CA)

    2012-07-17T23:59:59.000Z

    Diagnostic and therapeutic applications for Noonan Syndrome are described. The diagnostic and therapeutic applications are based on certain mutations in a RAS-specific guanine nucleotide exchange factor gene SOS1 or its expression product. The diagnostic and therapeutic applications are also based on certain mutations in a serine/threonine protein kinase gene RAF1 or its expression product thereof. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to RAF1 or SOS1, and variants thereof, as well as host cells expressing such variants.

  9. Analysis on the energy efficiency of variable-frequency air conditioners (Hitachi models as an example) Jim Jr-Min Lin 2014.09.26

    E-Print Network [OSTI]

    Analysis on the energy efficiency of variable-frequency air conditioners (Hitachi models (Max) Energy Efficiency @min load Energy Efficiency @Max load kW kW kW kW W/W W/W RAS-22NB 1.00 3.20 0 Efficiency @min load Energy Efficiency @Max load kW kW kW kW W/W W/W RAM-5FNS(B) - 12.5 - 2.91 - 4.3 RAM-6FNS

  10. The El Salvadoran refugee move to Belize: a geographic study of refugee migration

    E-Print Network [OSTI]

    McElroy, Charles Scott

    1986-01-01T23:59:59.000Z

    information on the individual refugee not alluded to in the schedule-based survey, was available through the United States Agency for International Development (USAID). Their study, entitled El Salvad r: A ricul- tural ect r Asses en 1 77 (1977), provides... use and ownership of the land by the majority rural poor are central to the future political and economic life of El Salvador. Thomas R Anderson's T e W r of he Di e Hond ras nd E~ll 968 (l981) * i I gi 1 dit' *' I ' Fl S I 4 that resulted...

  11. A high frequency resonance gravity gradiometer

    SciTech Connect (OSTI)

    Bagaev, S. N.; Kvashnin, N. L.; Skvortsov, M. N. [Laser Physics Institute SB RAS, Novosibirsc (Russian Federation); Bezrukov, L. B.; Krysanov, V. A. [Institute of Nuclear Physics RAS, Moscow (Russian Federation); Oreshkin, S. I.; Motylev, A. M.; Popov, S. M.; Samoilenko, A. A.; Yudin, I. S. [Lomonosov MSU, Sternberg Astronomical Institute, Moscow (Russian Federation); Rudenko, V. N. [Institute of Nuclear Physics RAS, Moscow (Russian Federation); Lomonosov MSU, Sternberg Astronomical Institute, Moscow (Russian Federation)

    2014-06-15T23:59:59.000Z

    A new setup OGRAN—the large scale opto-acoustical gravitational detector is described. As distinguished from known gravitational bar detectors it uses the optical interferometrical readout for registering weak variations of gravity gradient at the kilohetz frequency region. At room temperature, its sensitivity is limited only by the bar Brownian noise at the bandwidth close to 100 Hz. It is destined for a search for rare events—gravitational pulses coincident with signals of neutrino scintillator (BUST) in the deep underground of Baksan Neutrino Observatory of INR RAS.

  12. "Skinny Milky Way please", says Sagittarius

    E-Print Network [OSTI]

    Gibbons, S. L. J.; Belokurov, V.; Evans, N. W.

    2014-01-01T23:59:59.000Z

    that we do not tie the circular speed at the Solar radius to the fitted halo model as this induces a bias at small Galactocentric radii of the order of 10%. c© 2014 RAS, MNRAS 000, 1–16 “Skinny Milky Way”, says Sagittarius. 3 Figure 1. The formation of a... tidal stream in the energy and angular momentum space. Each of the three panels show snapshots of an N-body simulation in E and L space at different time steps along the progenitor’s orbit, namely at pericentre (left), between pericentre and apocentre...

  13. The effect of a helium-oxygen atmosphere on chick embryo development and subsequent chick performance

    E-Print Network [OSTI]

    Valera, Juan

    1969-01-01T23:59:59.000Z

    !ie hc I ium-oxygen groups r ere stroller t!ian embryos from the air group after 12 and 16 darys of development. The util is- tion of yolt& and al!'umen by the helium-oxygen etibryos during incu!ration r;as less than for the air grou... and reorod. ctive performance of chicks hatched in a helium- omycen atmosphere showed es -entially no differences when compared with c!sicks of the same ape hatched in the a ir chambers. physiolosical seasure. cnts, body weights, heso, lobin, hemntocr its...

  14. Peat as an energy alternative

    SciTech Connect (OSTI)

    Not Available

    1980-01-01T23:59:59.000Z

    This symposium, Peat as an Energy Alternative, was organized to facilitate in-depth discussion of the various topics at the last conference. The scope of the symposium was international and included papers from Brazil, Canada, Finland, Israel, Jamaica, and Sweden. The presentations were designed to provide the latest information relating to the following areas of interest: peat resources; peat deposit location; countries using peat for energy; countries showing inerest in considering peat for energy; status of the technologies for harvesting, dewatering, beneficiation, combustion, and conversion to synthetic fuels; environmental and socioeconomic issues relating to large-scale peat utilization; peat reclamation options; Federal and State government plans for peat utilization; and organizations working on developments relating to peat as an energy alternative. The symposium, sponsored by the Institute of Gas Technology, was held at Arlington, VA, December 1-3, 1980. Forth-six papers have been entered individually into EDB and ten also into ERA; two paper had been entered previously from other sources. (LTN)

  15. A limited assessment and characterization of the solar radiation energy resources in the Caribbean region

    SciTech Connect (OSTI)

    Hulstrom, R.L.

    1988-02-01T23:59:59.000Z

    The objective of our work was to produce a preliminary assessment and characterization of the Caribbean region (Barbados, Dominican Republic, Guatemala, Jamaica, and Panama) solar radiation energy resources. Such information will be used to estimate the performance of, and identify the most promising applications of, solar heat technologies in the Caribbean region. We expect the solar radiation resources in the Caribbean region to be very location specific. Sunny areas will have an annual direct-beam resource of about 3,000 kWhm/sup /minus 2// and a global solar radiation resource of about 2,500 kWhm/sup /minus 2//. Cloud-covered areas will have annual solar radiation resources of about 1,500 kWhm/sup /minus 2/ for both the direct-beam and the global solar radiation. Monthly levels of solar radiaion will vary markedly, ranging from an average of 9 to 3 kWhm/sup /minus 2//day/sup /minus 1// for the direct-beam and from an average of 7 to 4 kWhm/sup /minus 2//day/sup /minus 1// for the global solar radiation. The Caribbean region is comparable to the Great Plains region of the US, in terms of annual solar radiation resources; however, thorough ''prospecting'' is required to avoid areas having very low amounts of solar radiation.

  16. Annotated bibliography of coal in the Caribbean region. [Lignite

    SciTech Connect (OSTI)

    Orndorff, R.C.

    1985-01-01T23:59:59.000Z

    The purpose of preparing this annotated bibliography was to compile information on coal localities for the Caribbean region used for preparation of a coal map of the region. Also, it serves as a brief reference list of publications for future coal studies in the Caribbean region. It is in no way an exhaustive study or complete listing of coal literature for the Caribbean. All the material was gathered from published literature with the exception of information from Cuba which was supplied from a study by Gordon Wood of the US Geological Survey, Branch of Coal Resources. Following the classification system of the US Geological Survey (Wood and others, 1983), the term coal resources has been used in this report for reference to general estimates of coal quantities even though authors of the material being annotated may have used the term coal reserves in a similar denotation. The literature ranges from 1857 to 1981. The countries listed include Colombia, Mexico, Venezuela, Cuba, the Dominican Republic, Haiti, Jamaica, Puerto Rico, and the countries of Central America.

  17. Activation of the MAP Kinase Cascade by Exogenous Calcium-Sensing Receptor

    SciTech Connect (OSTI)

    Hobson, Susan A.; Wright, Jay W.; Lee, Fred; Mcneil, Scott; Bilderback, Tim R.; Rodland, Karin D.

    2003-02-01T23:59:59.000Z

    In Rat-1 fibroblasts and ovarian surface epithelial cells, extracellular calcium induces a proliferative response which appears to be mediated by the G-protein coupled Calcium-sensing Receptor (CaR), as expression of the non-functional CaR-R795W mutant inhibits both thymidine incorporation and activation of the extracellular-regulated kinase (ERK) in response to calcium. In this report we utilized CaR-transfected HEK293 cells to demonstrate that functional CaR is necessary and sufficient for calcium-induced ERK activation. CaR-dependent ERK activation was blocked by co-expression of the Ras dominant-negative mutant, Ras N17, and by exposure to the phosphatidyl inositol 3' kinase inhibitors wortmannin and LY294002. In contrast to Rat-1 fibroblasts, CaR-mediated in vitro kinase activity of ERK2 was unaffected by tyrosine kinase inhibitor herbimycin in CaR-transfected HEK293 cells. These results suggest that usage of distinct pathways downstream of the CaR varies in a cell-type specific manner, suggesting a potential mechanism by which activation of the CaR could couple to distinct calcium-dependent responses.

  18. Evolution of gas processing industry in Saudi Arabia

    SciTech Connect (OSTI)

    Showail, A.

    1983-01-01T23:59:59.000Z

    The beginning of the natural gas processing industry in Saudi Arabia is traced back to 1959 when Aramco embarked on a program to recover natural gas liquids (NGL) for export from low pressure gases such as stabilizer overhead, spheroid, tank farm, and refinery off-gases. The processing scheme involves compression and refrigeration to extract C3+ raw NGL, a raw NGL gathering system, and a fractionation plant to separate propane, butane, and natural gasoline. NGL extracted in Abqaiq and Ras Tanura is moved to Ras Tanura for fractionation, storage, and export. The system, built in several increments, has total design capacity of 500 MMscfd of feed gases to produce 320,000 bpd of NGL composed of 40% propane, 30% butane, and 30% natural gasoline. Phase II of the Saudi gas program envisages collection and processing of associated gas produced with Arabian medium and heavy crude oils largely in the northern onshore and offshore fields. Further domestic development may focus on more diversification in gas product utilization and on upgrading to higher value products.

  19. MEK1/2 dual-specificity protein kinases: Structure and regulation

    SciTech Connect (OSTI)

    Roskoski, Robert, E-mail: rrj@brimr.org [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)] [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)

    2012-01-06T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer MEK1 is activated by phosphorylation of S218 and S222 in its activation segment. Black-Right-Pointing-Pointer MEK1 activation requires KSR, which functions as a scaffold and a protein kinase. Black-Right-Pointing-Pointer S212 phosphorylation in the MEK1 activation segment is inhibitory. Black-Right-Pointing-Pointer MEK1 and MEK2 contain a catalytic and a regulatory spine. -- Abstract: MEK1 and MEK2 are related protein kinases that participate in the RAS-RAF-MEK-ERK signal transduction cascade. This cascade participates in the regulation of a large variety of processes including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Moreover, oncogenic mutations in RAS or B-RAF are responsible for a large proportion of human cancers. MEK1 is activated by phosphorylation of S218 and S222 in its activation segment as catalyzed by RAF kinases in an intricate process that involves a KSR scaffold. Besides functioning as a scaffold, the kinase activity of KSR is also required for MEK activation. MEK1 regulation is unusual in that S212 phosphorylation in its activation segment is inhibitory. Moreover, active ERK catalyzes a feedback inhibitory phosphorylation of MEK1 T292 that serves to downregulate the pathway.

  20. Prorenin induces ERK activation in endothelial cells to enhance neovascularization independently of the renin-angiotensin system

    SciTech Connect (OSTI)

    Uraoka, Maki [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)] [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan); Ikeda, Koji, E-mail: ikedak@koto.kpu-m.ac.jp [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)] [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan); Nakagawa, Yusuke; Koide, Masahiro; Akakabe, Yoshiki; Nakano-Kurimoto, Ritsuko; Takahashi, Tomosaburo; Matoba, Satoaki; Yamada, Hiroyuki; Okigaki, Mitsuhiko; Matsubara, Hiroaki [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)] [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)

    2009-12-25T23:59:59.000Z

    Prorenin is an enzymatically inactive precursor of renin, and its biological function in endothelial cells (ECs) is unknown despite its relevance with the incidence of diabetic microvascular complications. Recently, (pro)renin receptor was identified, and the receptor-associated prorenin system has been discovered, whereas its expression as well as function in ECs remain unclear. In the present study, we found that ECs express the (pro)renin receptor, and that prorenin provoked ERK activation through (pro)renin receptor independently of the renin-angiotensin system (RAS). Prorenin stimulated the proliferation, migration and tube-formation of ECs, while it inhibited endothelial apoptosis induced by serum and growth factor depletion. MEK inhibitor abrogated these proangiogenic effects of prorenin, while AT1 receptor antagonist or angiotensin-converting enzyme inhibitor failed to block them. In vivo neovascularization in the Matrigel-plugs implanted into mouse flanks was significantly enhanced by prorenin, in which significant ERK activation was detected in ECs. Furthermore, tumor xenografts stably transfected with prorenin demonstrated the significantly accelerated growth rate concomitantly with enhanced intratumoral neovascularization. Our data demonstrated that the RAS-independent (pro)renin receptor-mediated signal transduction plays a pivotal role in the regulation of ECs function as well as in the neovascularization, and thus prorenin is potentially involved in the pathophysiology of diabetic microvascular complications as well as cancers.

  1. TBC-Domain GAPs for Rab GTPases Accelerate GTP Hydrolysis by a Dual-Finger Mechanism

    SciTech Connect (OSTI)

    Pan,X.; Eathiraj, S.; Lambright, D.

    2006-01-01T23:59:59.000Z

    Rab GTPases regulate membrane trafficking by cycling between inactive (GDP-bound) and active (GTP-bound) conformations. The duration of the active state is limited by GTPase-activating proteins (GAPs), which accelerate the slow intrinsic rate of GTP hydrolysis. Proteins containing TBC (Tre-2, Bub2 and Cdc16) domains are broadly conserved in eukaryotic organisms and function as GAPs for Rab GTPases as well as GTPases that control cytokinesis. An exposed arginine residue is a critical determinant of GAP activity in vitro and in vivo. It has been expected that the catalytic mechanism of TBC domains would parallel that of Ras and Rho family GAPs. Here we report crystallographic, mutational and functional analyses of complexes between Rab GTPases and the TBC domain of Gyp1p. In the crystal structure of a TBC-domain-Rab-GTPase-aluminium fluoride complex, which approximates the transition-state intermediate for GTP hydrolysis, the TBC domain supplies two catalytic residues in trans, an arginine finger analogous to Ras/Rho family GAPs and a glutamine finger that substitutes for the glutamine in the DxxGQ motif of the GTPase. The glutamine from the Rab GTPase does not stabilize the transition state as expected but instead interacts with the TBC domain. Strong conservation of both catalytic fingers indicates that most TBC-domain GAPs may accelerate GTP hydrolysis by a similar dual-finger mechanism.

  2. Calcination/dissolution chemistry development Fiscal year 1995

    SciTech Connect (OSTI)

    Delegard, C.H.

    1995-09-01T23:59:59.000Z

    The task {open_quotes}IPC Liaison and Chemistry of Thermal Reconstitution{close_quotes} is a $300,000 program that was conducted in Fiscal Year (FY) 1995 with U.S. Department of Energy (DOE) Office of Research and Development (EM-53) Efficient Separations and Processing Crosscutting Program supported under technical task plan (TTP) RL4-3-20-04. The principal investigator was Cal Delegard of the Westinghouse Hanford Company (WHC). The task encompassed the following two subtasks related to the chemistry of alkaline Hanford Site tank waste: (1) Technical Liaison with the Institute of Physical Chemistry of the Russian Academy of Science (IPC/RAS) and its research into the chemistry of transuranic elements (TRU) and technetium (Tc) in alkaline media. (2) Laboratory investigation of the chemistry of calcination/dissolution (C/D) (or thermal reconstitution) as an alternative to the present reference Hanford Site tank waste pretreatment flowsheet, Enhanced Sludge Washing (ESW). This report fulfills the milestone for the C/D subtask to {open_quotes}Provide End-of-Year Report on C/D Laboratory Test Results{close_quotes} due 30 September 1995. A companion report, fulfilling the milestone to provide an end-of-year report on the IPC/RAS liaison, also has been prepared.

  3. Remedial action plan and site design for stabilization of the inactive uranium processing site at Naturita, Colorado. Remedial Action Selection Report, Appendix B of Attachment 2: Geology report, Final

    SciTech Connect (OSTI)

    Not Available

    1994-03-01T23:59:59.000Z

    The uranium processing site near Naturita, Colorado, is one of 24 inactive uranium mill sites designated to be cleaned up by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA), 42 USC {section} 7901 et seq. Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which describes the proposed remedial action for the Naturita site. An extensive amount of data and supporting information has been generated and evaluated for this remedial action. These data and supporting information are not incorporated into this single document but are included or referenced in the supporting documents. The RAP consists of this RAS and four supporting documents or attachments. This Attachment 2, Geology Report describes the details of geologic, geomorphic, and seismic conditions at the Dry Flats disposal site.

  4. Soil Management Plan for the Oak Ridge Y-12 National Security Complex Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    None

    2005-03-02T23:59:59.000Z

    This Soil Management Plan applies to all activities conducted under the auspices of the National Nuclear Security Administration (NNSA) Oak Ridge Y-12 National Security Complex (Y-12) that involve soil disturbance and potential management of waste soil. The plan was prepared under the direction of the Y-12 Environmental Compliance Department of the Environment, Safety, and Health Division. Soil disturbances related to maintenance activities, utility and building construction projects, or demolition projects fall within the purview of the plan. This Soil Management Plan represents an integrated, visually oriented, planning and information resource tool for decision making involving excavation or disturbance of soil at Y-12. This Soil Management Plan addresses three primary elements. (1) Regulatory and programmatic requirements for management of soil based on the location of a soil disturbance project and/or the regulatory classification of any contaminants that may be present (Chap. 2). Five general regulatory or programmatic classifications of soil are recognized to be potentially present at Y-12; soil may fall under one or more these classifications: (a) Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) pursuant to the Oak Ridge Reservation (ORR) Federal Facilities Agreement; (b) Resource Conservation and Recovery Act (RCRA); (c) RCRA 3004(u) solid waste managements units pursuant to the RCRA Hazardous and Solid Waste Amendments Act of 1984 permit for the ORR; (d) Toxic Substances and Control Act-regulated soil containing polychlorinated biphenyls; and (e) Radiologically contaminated soil regulated under the Atomic Energy Act review process. (2) Information for project planners on current and future planned remedial actions (RAs), as prescribed by CERCLA decision documents (including the scope of the actions and remedial goals), land use controls implemented to support or maintain RAs, RCRA post-closure regulatory requirements for former waste management units, legacy contamination source areas and distribution of contamination in soils, and environmental infrastructure (e.g., caps, monitoring systems, etc.) that is in place or planned in association with RAs. (3) Regulatory considerations and processes for management and disposition of waste soil upon generation, including regulatory drivers, best management practices (BMPs), waste determination protocols, waste acceptance criteria, and existing waste management procedures and BMPs for Y-12. This Soil Management Plan provides information to project planners to better coordinate their activities with other organizations and programs with a vested interest in soil disturbance activities at Y-12. The information allows project managers and maintenance personnel to evaluate and anticipate potential contaminant levels that may be present at a proposed soil disturbance site prior to commencement of activities and allows a more accurate assessment of potential waste management requirements.

  5. An investigation of the applicability of the photo-electric cell to the determination of solubility

    E-Print Network [OSTI]

    Nordsieck, Herbert Henry

    1936-01-01T23:59:59.000Z

    , sad silver chromato solutioas on ths intensity of light. Those 4sts sre represented bp ourvos in Charts II XVc VIy VIXI ~ YARIATIOE OF INTENSITY OF LIGHT WITS TIME FOR VARIOUS CONCl9lTRATIOMS OF SILVER CRLORmE 1. 00 x 10 aols A801 yor Kiter lo80... of RasGrOa mole 9or liter iisf lost isa soalo sivisisas Coassatrat ioa of Na, Cr0, mole psr liter Dsf loot ioa seals divisioas 0. 5 x 10"6 Qed X 10 O Oe9 x 10 0, 8 x10 Oe9x106 leOz105 l. lx106 12xlQ 1. 8 x 10-6 le4 x 10 led z 10 led x...

  6. Positive Feedback Regulation Results in Spatial Clustering and Fast Spreading of Active Signaling Molecules on a Cell Membrane

    E-Print Network [OSTI]

    Jayajit Das; Mehran Kardar; Arup K. Chakraborty

    2009-06-29T23:59:59.000Z

    Positive feedback regulation is ubiquitous in cell signaling networks, often leading to binary outcomes in response to graded stimuli. However, the role of such feedbacks in clustering, and in spatial spreading of activated molecules, has come to be appreciated only recently. We focus on the latter, using a simple model developed in the context of Ras activation with competing negative and positive feedback mechanisms. We find that positive feedback, in the presence of slow diffusion, results in clustering of activated molecules on the plasma membrane, and rapid spatial spreading as the front of the cluster propagates with a constant velocity (dependent on the feedback strength). The advancing fronts of the clusters of the activated species are rough, with scaling consistent with the Kardar-Parisi-Zhang (KPZ) equation in one dimension. Our minimal model is general enough to describe signal transduction in a wide variety of biological networks where activity in the membrane-proximal region is subject to feedback regulation.

  7. Fiscal Year 2007 Phased Construction Completion Report for the Zone 2 Soils, Slabs, and Subsurface Structures at East Tennessee Technology Park, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    RSI

    2008-03-01T23:59:59.000Z

    The purpose of this Phased Construction Completion Report (PCCR) is to present the fiscal year (FY) 2007 results of characterization activities and recommended remedial actions (RAs) for 11 exposure units (EUs) in Zone 2 (Z2-01, Z2-03, Z2-08, Z2-23, Z2-24, Z2-28, Z2-34, Z2-37, Z2-41, Z2-43, and Z2-44) at the East Tennessee Technology Park (ETTP), which is located in the northwest corner of the U.S. Department of Energy (DOE) Oak Ridge Reservation in Oak Ridge, Tennessee (Fig. 1). ETTP encompasses a total land area of approximately 5000 acres that has been subdivided into three zones--Zone 1 ({approx}1400 acres), Zone 2 ({approx}800 acres), and the Boundary Area ({approx}2800 acres). Zone 2, which encompasses the highly industrialized portion of ETTP shown in Fig. 1, consists of all formerly secured areas of the facility, including the large processing buildings and direct support facilities; experimental laboratories and chemical and materials handling facilities; materials storage and waste disposal facilities; secure document records libraries; and shipping and receiving warehouses. The Zone 2 Record of Decision for Soil, Buried Waste, and Subsurface Structure Actions in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE 2005) (Zone 2 ROD) specifies the future end use for Zone 2 acreage as uncontrolled industrial for the upper 10 ft of soils. Characterization activities in these areas were conducted in compliance with the Zone 2 ROD and the Dynamic Verification Strategy (DVS) and data quality objectives (DQOs) presented in the Remedial Design Report/Remedial Action Work Plan for Zone 2 Soils, Slabs, and Subsurface Structures, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE 2007) (Zone 2 RDR/RAWP). The purpose of this PCCR is to address the following: (1) Document DVS characterization results for the accessible EUs in FY 2007; (2) Describe and document the risk evaluation for each EU, and determine if the EU met the Zone 2 ROD requirements for unrestricted industrial use to 10 ft bgs; (3) Identify additional areas not defined in the Zone 2 ROD that require remediation based on the DVS evaluation results; and (4) Describe the RAs performed in Zone 2. The Zone 2 ROD divided the Zone 2 area into 7 geographic areas and 44 EUs. To facilitate the DQOs of the DVS process, the Zone 2 RDR/RAWP regrouped the 44 EUs into 12 DQO scoping EU groups. These groups facilitated the DQO process by placing similar facilities and their support facilities together and allowed identification of data gaps. The EU groups were no longer pertinent after DQO planning was completed, and characterization was conducted as areas became accessible. As the opportunity to complete characterization became available, the planned DVS program was executed and completed in FY 2007 for the 11 EUs addressed in this document. The main body of this report describes both the DVS process and scope of work performed and the RAs completed. The scope and approach for performing DVS activities performed in FY 2007 that lead to action/no further action decisions are presented in Sects. 2 through 4. RAs performed in FY 2007 are presented in Sects. 5 through 10. Future land use is described in Sect. 11, and the status of all Zone 2 EUs as of this PCCR is presented in Sect. 12.

  8. Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins

    SciTech Connect (OSTI)

    Deng, Lin [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China) [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China); Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710038 (China); Lu, Yuanyuan; Zhao, Xiaodi; Sun, Yi; Shi, Yongquan; Fan, Hongwei; Liu, Changhao; Zhou, Jinfeng; Nie, Yongzhan; Wu, Kaichun [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China)] [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China); Fan, Daiming, E-mail: daimingfan@fmmu.edu.cn [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China)] [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China); Guo, Xuegang, E-mail: xuegangguo@126.com [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China)] [State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, Shaanxi 710032 (China)

    2013-10-18T23:59:59.000Z

    Highlights: •Overexpression of Ran in pancreatic cancer was correlated with histological grade. •Downregulation of Ran could induce cell apoptosis and inhibit cell proliferation. •The effects were mediated by cell cycle proteins, Survivin and cleaved Caspase-3. -- Abstract: Ran, a member of the Ras GTPase family, has important roles in nucleocytoplasmic transport. Herein, we detected Ran expression in pancreatic cancer and explored its potential role on tumour progression. Overexpressed Ran in pancreatic cancer tissues was found highly correlated with the histological grade. Downregulation of Ran led to significant suppression of cell proliferation, cell cycle arrest at the G1/S phase and induction of apoptosis. In vivo studies also validated that result. Further studies revealed that those effects were at least partly mediated by the downregulation of Cyclin A, Cyclin D1, Cyclin E, CDK2, CDK4, phospho-Rb and Survivin proteins and up regulation of cleaved Caspase-3.

  9. Nested methylation-specific polymerase chain reaction cancer detection method

    DOE Patents [OSTI]

    Belinsky, Steven A. (Albuquerque, NM); Palmisano, William A. (Edgewood, NM)

    2007-05-08T23:59:59.000Z

    A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.

  10. Computational chemistry in Argonne`s Reactor Analysis Division

    SciTech Connect (OSTI)

    Gelbard, E.; Agrawal, R.; Fanning, T.

    1997-08-01T23:59:59.000Z

    Roughly 3 years ago work on Argonne`s Integral Fast Reactor ({open_quotes}IFR{close_quotes}) was terminated and at that time, ANL funding was redirected to a number of alternative programs. One such alternative was waste management and, since disposal of spent fuel from ANL`s EBR-II reactor presents some special problems, this seemed an appropriate area for ANL work. Methods for the treatment and disposal of spent fuel (particularly from EBR-II but also from other sources) are now under very active investigation at ANL. The very large waste form development program is mainly experimental at this point, but within the Reactor Analysis ({open_quotes}RA{close_quotes}) Division a small computational chemistry program is underway, designed to supplement the experimental program. One of the most popular proposals for the treatment of much of our high-level wastes is vitrification. As noted below, this approach has serious drawbacks for EBR-II spent fuel. ANL has proposed, instead, that spent fuel first be pretreated by a special metallurgical process which produces, as waste, chloride salts of the various fission products; these salts would then be adsorbed in zeolite A, which is subsequently bonded with glass to produce a waste form suitable for disposal. So far it has been the main mission of RA`s computational chemistry program to study the process by which leaching occurs when the glass-bonded zeolite waste form is exposed to water. It is the purpose of this paper to describe RA`s computational chemistry program, to discuss the computational techniques involved in such a program, and in general to familiarize the M. and C. Division with a computational area which is probably unfamiliar to most of its member. 11 refs., 2 figs.

  11. Losartan attenuates chronic cigarette smoke exposure-induced pulmonary arterial hypertension in rats: Possible involvement of angiotensin-converting enzyme-2

    SciTech Connect (OSTI)

    Han Suxia; He Guangming; Wang Tao; Chen Lei; Ning Yunye; Luo Feng; An Jin; Yang Ting; Dong Jiajia; Liao Zenglin; Xu Dan [Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, and Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041 (China); Wen Fuqiang, E-mail: wenfuqiang.scu@gmail.co [Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, and Department of Respiratory Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan 610041 (China)

    2010-05-15T23:59:59.000Z

    Chronic cigarette smoking induces pulmonary arterial hypertension (PAH) by largely unknown mechanisms. Renin-angiotensin system (RAS) is known to function in the development of PAH. Losartan, a specific angiotensin II receptor antagonist, is a well-known antihypertensive drug with a potential role in regulating angiotensin-converting enzyme-2 (ACE2), a recently found regulator of RAS. To determine the effect of losartan on smoke-induced PAH and its possible mechanism, rats were daily exposed to cigarette smoke for 6 months in the absence and in the presence of losartan. Elevated right ventricular systolic pressure (RVSP), thickened wall of pulmonary arteries with apparent medial hypertrophy along with increased angiotensin II (Ang II) and decreased ACE2 levels were observed in smoke-exposed-only rats. Losartan administration ameliorated pulmonary vascular remodeling, inhibited the smoke-induced RVSP and Ang II elevation and partially reversed the ACE2 decrease in rat lungs. In cultured primary pulmonary artery smooth muscle cells (PASMCs) from 3- and 6-month smoke-exposed rats, ACE2 levels were significantly lower than in those from the control rats. Moreover, PASMCs from 6-month exposed rats proliferated more rapidly than those from 3-month exposed or control rats, and cells grew even more rapidly in the presence of DX600, an ACE2 inhibitor. Consistent with the in vivo study, in vitro losartan pretreatment also inhibited cigarette smoke extract (CSE)-induced cell proliferation and ACE2 reduction in rat PASMCs. The results suggest that losartan may be therapeutically useful in the chronic smoking-induced pulmonary vascular remodeling and PAH and ACE2 may be involved as part of its mechanism. Our study might provide insight into the development of new therapeutic interventions for PAH smokers.

  12. Temperature Studies for ATLAS MDT BOS Chambers

    E-Print Network [OSTI]

    A. Engl; O. Biebel; R. Hertenberger; R. Mameghani; D. Merkl; F. Rauscher; D. Schaile; R. Stroehmer

    2009-08-11T23:59:59.000Z

    Data sets with high statistics taken at the cosmic ray facility, equipped with 3 ATLAS BOS MDT chambers, in Garching (Munich) have been used to study temperature and pressure effects on gas gain and drifttime. The deformation of a thermally expanded chamber was reconstructed using the internal RasNik alignment monitoring system and the tracks from cosmic data. For these studies a heating system was designed to increase the temperature of the middle chamber by up to 20 Kelvins over room temperature. For comparison the temperature effects on gas properties have been simulated with Garfield. The maximum drifttime decreased under temperature raise by -2.21 +- 0.08 ns/K, in agreement with the results of pressure variations and the Garfield simulation. The increased temperatures led to a linear increase of the gas gain of about 2.1% 1/K. The chamber deformation has been analyzed with the help of reconstructed tracks. By the comparison of the tracks through the reference chambers with these through the test chamber the thermal expansion has been reconstructed and the result shows agreement with the theoretical expansion coefficient. As the wires are fixed at the end of the chamber, the wire position calculation can not provide a conclusion for the chamber middle. The complete deformation has been identified with the analysis of the monitoring system RasNik, whose measured values have shown a homogeneous expansion of the whole chamber, overlayed by a shift and a rotation of the chamber middle with respect to the outer part of the chamber. The established results of both methods are in agreement. We present as well a model for the position-drifttime correction as function of temperature.

  13. Report on the research conducted under the funding of the Sloan foundation postdoctoral fellowship in Computational Molecular Biology [Systematic study of protein-protein complexes] Final report

    SciTech Connect (OSTI)

    Sheinerman, Felix

    2001-06-01T23:59:59.000Z

    A central question in molecular biology is what structural features are common at protein-protein interfaces and what energetic factors define the affinity and specificity of protein-protein association. Analysis of structural and mutational data on protein-protein interfaces revealed that protein-protein interfaces of different functional classes contain many more energetically important charged and polar residues than was previously thought. Since, in the context of protein folding studies, polar interactions are believed to destabilize the folded proteins, this observation raised the question as to the forces that determine the stability of protein complexes. To investigate this issue in detail, the authors developed a number of partitioning schemes that allowed them to investigate the role of selected residues, ion pairs, and networks of polar interactions in protein-protein association. The methods developed were applied to the analysis of four different protein-protein interfaces: the ribonuclease barnase and its inhibitor barstar, the human growth hormone and its receptor, subtype N9 influenze virus neuraminidase and NC41 antibody, and the Ras Binding Domain of kinase cRaf and a Ras homologue Rap1A. The calculations revealed a surprising variability in how polar interactions affect the stability of different complexes. The finding that positions of charged and polar residues on protein-protein interfaces are optimized with respect to electrostatic interactions suggests that this property can be employed for the discrimination between native conformations and trial complexes generated by a docking algorithm. Analysis indicated the presence of SH2 domains in Janus family of non-receptor protein tyrosine kinases.

  14. The BRAF{sup T1799A} mutation confers sensitivity of thyroid cancer cells to the BRAF{sup V600E} inhibitor PLX4032 (RG7204)

    SciTech Connect (OSTI)

    Xing, Joanna [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)] [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States); Liu, Ruixin; Xing, Mingzhao [Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, MD 21287 (United States)] [Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, MD 21287 (United States); Trink, Barry, E-mail: btrink@jhmi.edu [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)] [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)

    2011-01-28T23:59:59.000Z

    Research highlights: {yields} Exciting therapeutic potential has been recently reported for the BRAF{sup V600E} inhibitor PLX4032 in melanoma. {yields} We tested the effects of PLX4032 on the growth of thyroid cancer cells which often harbor the BRAF{sup V600E} mutation. {yields} We observed a potent BRAF{sup V600E}-dependent inhibition of thyroid cancer cells by PLX4032. {yields} We thus demonstrated an important therapeutic potential of PLX4032 for thyroid cancer. -- Abstract: Aberrant signaling of the Ras-Raf-MEK-ERK (MAP kinase) pathway driven by the mutant kinase BRAF{sup V600E}, as a result of the BRAF{sup T1799A} mutation, plays a fundamental role in thyroid tumorigenesis. This study investigated the therapeutic potential of a BRAF{sup V600E}-selective inhibitor, PLX4032 (RG7204), for thyroid cancer by examining its effects on the MAP kinase signaling and proliferation of 10 thyroid cancer cell lines with wild-type BRAF or BRAF{sup T1799A} mutation. We found that PLX4032 could effectively inhibit the MAP kinase signaling, as reflected by the suppression of ERK phosphorylation, in cells harboring the BRAF{sup T1799A} mutation. PLX4032 also showed a potent and BRAF mutation-selective inhibition of cell proliferation in a concentration-dependent manner. PLX4032 displayed low IC{sub 50} values (0.115-1.156 {mu}M) in BRAF{sup V600E} mutant cells, in contrast with wild-type BRAF cells that showed resistance to the inhibitor with high IC{sub 50} values (56.674-1349.788 {mu}M). Interestingly, cells with Ras mutations were also sensitive to PLX4032, albeit moderately. Thus, this study has confirmed that the BRAF{sup T1799A} mutation confers cancer cells sensitivity to PLX4032 and demonstrated its specific potential as an effective and BRAF{sup T1799A} mutation-selective therapeutic agent for thyroid cancer.

  15. Azathioprine desensitizes liver cancer cells to insulin-like growth factor 1 and causes apoptosis when it is combined with bafilomycin A1

    SciTech Connect (OSTI)

    Hernández-Breijo, Borja [Departamento de Biología de Sistemas, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); Monserrat, Jorge [Departamento de Medicina y Especialidades Médicas, Universidad de Alcalá, 28871 Alcalá de Henares (Spain); Román, Irene D. [Departamento de Biología de Sistemas, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); González-Rodríguez, Águeda [Departamento de Biomedicina y Biotecnología, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); Fernández-Moreno, M. Dolores [Departamento de Biología de Sistemas, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); and others

    2013-11-01T23:59:59.000Z

    Hepatoblastoma is a primary liver cancer that affects children, due to the sensitivity of this tumor to insulin-like growth factor 1 (IGF-1). In this paper we show that azathioprine (AZA) is capable of inhibiting IGF1-mediated signaling cascade in HepG2 cells. The efficiency of AZA on inhibition of proliferation differs in the evaluated cell lines as follows: HepG2 (an experimental model of hepatoblastoma) > Hep3B (derived from a hepatocellular carcinoma) > HuH6 (derived from a hepatoblastoma) ? HuH7 (derived from a hepatocellular carcinoma) = Chang Liver cells (a non-malignant cellular model). The effect of AZA in HepG2 cells has been proven to derive from activation of Ras/ERK/TSC2, leading to activation of mTOR/p70S6K in a sustained manner. p70S6K phosphorylates IRS-1 in serine 307 which leads to the uncoupling between IRS-1 and p85 (the regulatory subunit of PI3K) and therefore causing the lack of response of HepG2 to IGF-1. As a consequence, proliferation induced by IGF-1 is inhibited by AZA and autophagy increases leading to senescence of HepG2 cells. Our results suggest that AZA induces the autophagic process in HepG2 activating senescence, and driving to deceleration of cell cycle but not to apoptosis. However, when simultaneous to AZA treatment the autophagy was inhibited by bafilomycin A1 and the degradation of regulatory proteins of cell cycle (e.g. Rb, E2F, and cyclin D1) provoked apoptosis. In conclusion, AZA induces resistance in hepatoblastoma cells to IGF-1, which leads to autophagy activation, and causes apoptosis when it is combined with bafilomycin A1. We are presenting here a novel mechanism of action of azathioprine, which could be useful in treatment of IGF-1 dependent tumors, especially in its combination with other drugs. - Highlights: • Azathioprine activated Ras/ERK/TSC-2/mTOR/p70S6K signaling pathway in HepG2 cells. • Azathioprine inhibited IGF-1-mediated signaling cascade. • Azathioprine induced autophagy leading to cell cycle arrest. • Cells died by apoptosis when azathioprine was combined with bafilomycin A1.

  16. The oncogenic action of ionizing radiation on rat skin. Final progress report, May 1, 1990--April 30, 1992

    SciTech Connect (OSTI)

    Burns, F.J.; Garte, S.J.

    1992-12-31T23:59:59.000Z

    The multistage theory of carcinogenesis specifies that cells progress to cancer through a series of discrete, irreversible genetic alterations, but data on radiation-induced cancer incidence in rat skin suggests that an intermediate repairable alteration may occur. Data are presented on cancer induction in rat skin exposed to an electron beam (LET=0.34 keV/{mu}), a neon ion beam (LET=45) or an argon ion beam (LET=125). The rats were observed for tumors at least 78 weeks with squamous and basal cell carcinomas observed. The total cancer yield was fitted by the quadratic equation, and the equation parameters were estimated by linear regression for each type of radiation. Analysis of the DNA from the electron-induced carcinomas indicated that K-ras and/or c-myc oncogenes were activated. In situ hybridization indicated that the cancers contain subpopulations of cells with differing amounts of c-myc and H-ras amplification. The results are consistent with the idea that ionizing radiation produces stable, carcinogenically relevant lesions via 2 repairable events at low LET and via a non-repairable linked event pathway at high LET; either pathway may advance the cell by 1 stage. The proliferative response of rat epidermis following exposure to ionizing radiation was quantified by injection of {sup 14}C-thymidine. The return of these cells to S-phase a second time was detected by a second label ({sup 3}H). When the labeled cells were in G1-phase, the dorsal skin was irradiated with X-rays. All labeling indices were determined. The {sup 14}C labeling index was constant and unaffected by the radiation. The proportion of all cells entering S-phase averaged 3.5% at 18 hr and increased after 44, 52 and 75 hr to average levels of 11.8%, 5. 3%, and 6.6% at 0, 10 and 25 Gy respectively. The proportion of S-phase cells labeled with {sup 14}C increased after 42 hr and remained relatively constant thereafter.

  17. The oncogenic action of ionizing radiation on rat skin

    SciTech Connect (OSTI)

    Burns, F.J.; Garte, S.J.

    1992-01-01T23:59:59.000Z

    The multistage theory of carcinogenesis specifies that cells progress to cancer through a series of discrete, irreversible genetic alterations, but data on radiation-induced cancer incidence in rat skin suggests that an intermediate repairable alteration may occur. Data are presented on cancer induction in rat skin exposed to an electron beam (LET=0.34 keV/[mu]), a neon ion beam (LET=45) or an argon ion beam (LET=125). The rats were observed for tumors at least 78 weeks with squamous and basal cell carcinomas observed. The total cancer yield was fitted by the quadratic equation, and the equation parameters were estimated by linear regression for each type of radiation. Analysis of the DNA from the electron-induced carcinomas indicated that K-ras and/or c-myc oncogenes were activated. In situ hybridization indicated that the cancers contain subpopulations of cells with differing amounts of c-myc and H-ras amplification. The results are consistent with the idea that ionizing radiation produces stable, carcinogenically relevant lesions via 2 repairable events at low LET and via a non-repairable linked event pathway at high LET; either pathway may advance the cell by 1 stage. The proliferative response of rat epidermis following exposure to ionizing radiation was quantified by injection of [sup 14]C-thymidine. The return of these cells to S-phase a second time was detected by a second label ([sup 3]H). When the labeled cells were in G1-phase, the dorsal skin was irradiated with X-rays. All labeling indices were determined. The [sup 14]C labeling index was constant and unaffected by the radiation. The proportion of all cells entering S-phase averaged 3.5% at 18 hr and increased after 44, 52 and 75 hr to average levels of 11.8%, 5. 3%, and 6.6% at 0, 10 and 25 Gy respectively. The proportion of S-phase cells labeled with [sup 14]C increased after 42 hr and remained relatively constant thereafter.

  18. Chronic cadmium exposure in vitro induces cancer cell characteristics in human lung cells

    SciTech Connect (OSTI)

    Person, Rachel J.; Tokar, Erik J.; Xu, Yuanyuan; Orihuela, Ruben; Ngalame, Ntube N. Olive; Waalkes, Michael P., E-mail: waalkes@niehs.nih.gov

    2013-12-01T23:59:59.000Z

    Cadmium is a known human lung carcinogen. Here, we attempt to develop an in vitro model of cadmium-induced human lung carcinogenesis by chronically exposing the peripheral lung epithelia cell line, HPL-1D, to a low level of cadmium. Cells were chronically exposed to 5 ?M cadmium, a noncytotoxic level, and monitored for acquired cancer characteristics. By 20 weeks of continuous cadmium exposure, these chronic cadmium treated lung (CCT-LC) cells showed marked increases in secreted MMP-2 activity (3.5-fold), invasion (3.4-fold), and colony formation in soft agar (2-fold). CCT-LC cells were hyperproliferative, grew well in serum-free media, and overexpressed cyclin D1. The CCT-LC cells also showed decreased expression of the tumor suppressor genes p16 and SLC38A3 at the protein levels. Also consistent with an acquired cancer cell phenotype, CCT-LC cells showed increased expression of the oncoproteins K-RAS and N-RAS as well as the epithelial-to-mesenchymal transition marker protein Vimentin. Metallothionein (MT) expression is increased by cadmium, and is typically overexpressed in human lung cancers. The major MT isoforms, MT-1A and MT-2A were elevated in CCT-LC cells. Oxidant adaptive response genes HO-1 and HIF-1A were also activated in CCT-LC cells. Expression of the metal transport genes ZNT-1, ZNT-5, and ZIP-8 increased in CCT-LC cells culminating in reduced cadmium accumulation, suggesting adaptation to the metal. Overall, these data suggest that exposure of human lung epithelial cells to cadmium causes acquisition of cancer cell characteristics. Furthermore, transformation occurs despite the cell's ability to adapt to chronic cadmium exposure. - Highlights: • Chronic cadmium exposure induces cancer cell characteristics in human lung cells. • This provides an in vitro model of cadmium-induced human lung cell transformation. • This occurred with general and lung specific changes typical for cancer cells. • These findings add insight to the relationship between cadmium and lung cancer.

  19. Cross-talk between non-genomic and genomic signalling pathways - Distinct effect profiles of environmental estrogens

    SciTech Connect (OSTI)

    Silva, Elisabete, E-mail: elisabete.silva@pharmacy.ac.u [Centre for Toxicology, School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX (United Kingdom); Kabil, Alena; Kortenkamp, Andreas [Centre for Toxicology, School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX (United Kingdom)

    2010-06-01T23:59:59.000Z

    Estrogen receptor (ER) transcriptional cross-talk after activation by 17{beta}-estradiol (E2) has been studied in considerable detail, but comparatively little is known about the ways in which synthetic estrogen-like chemicals, so-called xenoestrogens, interfere with these signalling pathways. E2 can stimulate rapid, non-genomic signalling events, such as activation of the Src/Ras/Erk signalling pathway. We investigated how activation of this pathway by E2, the estrogenic environmental contaminants o,p'-DDT, {beta}-HCH and p,p'-DDE, and epidermal growth factor (EGF) influences the expression of ER target genes, such as TFF1, ER, PR, BRCA1 and CCND1, and the proliferation of breast cancer cells. Despite commonalities in their estrogenicity as judged by cell proliferation assays, the environmental contaminants exhibited striking differences in their non-genomic and genomic signalling. The gene expression profiles of o,p'-DDT and {beta}-HCH resembled the effects observed with E2. In the case of {beta}-HCH this is surprising, considering its reported lack of affinity to the 'classical' ER. The expression profiles seen with p,p'-DDE showed some similarities with E2, but overall, p,p'-DDE was a fairly weak transcriptional inducer of TFF1, ER, PR, BRCA1 and CCND1. We observed distinct differences in the non-genomic signalling of the tested compounds. p,p'-DDE was unable to stimulate Src and Erk1/Erk2 activations. The effects of E2 on Src and Erk1/Erk2 phosphorylation were transient and weak when compared to EGF, but {beta}-HCH induced strong and sustained activation of all tested kinases. Transcription of TFF1, ER, PR and BRCA1 by E2, o,p'-DDT and {beta}-HCH could be suppressed partially by inhibiting the Src/Ras/Erk pathway with PD 98059. However, this was not seen with p,p'-DDE. Our investigations show that the cellular activities of estrogens and xenoestrogens are the result of a combination of extranuclear (non-genomic) and nuclear (genomic) events and highlight the need to take non-genomic effects and signalling cross-talk into consideration, when screening for environmental estrogens. Otherwise, chemicals devoid of ER affinity, such as {beta}-HCH, but with an effect profile otherwise similar to estrogens might be overlooked in safety testing.

  20. Chronic infusion of enalaprilat into hypothalamic paraventricular nucleus attenuates angiotensin II-induced hypertension and cardiac hypertrophy by restoring neurotransmitters and cytokines

    SciTech Connect (OSTI)

    Kang, Yu-Ming, E-mail: ykang@mail.xjtu.edu.cn [Department of Physiology and Pathophysiology, Xi'an Jiaotong University Cardiovascular Research Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061 (China); Zhang, Dong-Mei [Department of Physiology, Dalian Medical University, Dalian 116044 (China); Yu, Xiao-Jing; Yang, Qing; Qi, Jie; Su, Qing [Department of Physiology and Pathophysiology, Xi'an Jiaotong University Cardiovascular Research Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061 (China); Suo, Yu-Ping [Department of Obstetrics and Gynecology, Shanxi Provincial People's Hospital, Taiyuan 030012 (China); Yue, Li-Ying [Department of Physiology and Pathophysiology, Xi'an Jiaotong University Cardiovascular Research Center, Xi'an Jiaotong University School of Medicine, Xi'an 710061 (China); Zhu, Guo-Qing [Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Physiology, Nanjing Medical University, Nanjing 210029 (China); Qin, Da-Nian, E-mail: dnqin@stu.edu.cn [Department of Physiology, Shantou University Medical College, Shantou 515041 (China)

    2014-02-01T23:59:59.000Z

    The renin–angiotensin system (RAS) in the brain is involved in the pathogenesis of hypertension. We hypothesized that inhibition of angiotensin-converting enzyme (ACE) in the hypothalamic paraventricular nucleus (PVN) attenuates angiotensin II (ANG II)-induced hypertension via restoring neurotransmitters and cytokines. Rats underwent subcutaneous infusions of ANG II or saline and bilateral PVN infusions of ACE inhibitor enalaprilat (ENL, 2.5 ?g/h) or vehicle for 4 weeks. ANG II infusion resulted in higher mean arterial pressure and cardiac hypertrophy as indicated by increased whole heart weight/body weight ratio, whole heart weight/tibia length ratio, left ventricular weight/tibia length ratio, and mRNA expressions of cardiac atrial natriuretic peptide and beta-myosin heavy chain. These ANG II-infused rats had higher PVN levels of glutamate, norepinephrine, tyrosine hydroxylase, pro-inflammatory cytokines (PICs) and the chemokine monocyte chemoattractant protein-1, and lower PVN levels of gamma-aminobutyric acid, interleukin (IL)-10 and the 67-kDa isoform of glutamate decarboxylase (GAD67), and higher plasma levels of PICs, norepinephrine and aldosterone, and lower plasma IL-10, and higher renal sympathetic nerve activity. However, PVN treatment with ENL attenuated these changes. PVN microinjection of ANG II induced increases in IL-1? and IL-6, and a decrease in IL-10 in the PVN, and pretreatment with angiotensin II type 1 receptor (AT1-R) antagonist losartan attenuated these changes. These findings suggest that ANG II infusion induces an imbalance between excitatory and inhibitory neurotransmitters and an imbalance between pro- and anti-inflammatory cytokines in the PVN, and PVN inhibition of the RAS restores neurotransmitters and cytokines in the PVN, thereby attenuating ANG II-induced hypertension and cardiac hypertrophy. - Highlights: • Chronic ANG II infusion results in sympathetic hyperactivity and cardiac hypertrophy. • PVN inhibition of ACE attenuates ANG II-induced hypertension and cardiac hypertrophy. • PVN inhibition of ACE attenuates ANG II-induced imbalance of PVN neurotransmitters. • PVN inhibition of ACE attenuates ANG II-induced imbalance of cytokines in the PVN. • PVN blockade of AT1-R attenuates ANG II-induced imbalance of cytokines in the PVN.

  1. 2010 Remediation Effectiveness Report for the U.S. Department of Energy Oak Ridge Reservation, Oak Ridge, Tennessee - Data and Evaluations

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2010-09-01T23:59:59.000Z

    Under the requirements of the Oak Ridge Reservation (ORR) Federal Facility Agreement (FFA) established between the U.S. Department of Energy (DOE), the U.S. Environmental Protection Agency, (EPA) and the Tennessee Department of Environment and Conservation (TDEC) in 1992, all environmental restoration activities on the ORR are performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Since the 1990s, the environmental restoration activities have experienced a gradual shift from characterization to remediation. As this has occurred, it has been determined that the assessment of the individual and cumulative performance of all ORR CERCLA remedial actions (RAs) is most effectively tracked in a single document. The Remediation Effectiveness Report (RER) is an FFA document intended to collate all ORR CERCLA decision requirements, compare pre- and post-remediation conditions at CERCLA sites, and present the results of any required post-decision remediation effectiveness monitoring. First issued in 1997, the RER has been reissued annually to update the performance histories of completed actions and to add descriptions of new CERCLA actions. Monitoring information used in the 2010 RER to assess remedy performance was collected and/or compiled by DOE's Water Resources Restoration Program (WRRP). Only data used to assess performance of completed actions are provided. In addition to collecting CERCLA performance assessment data, the WRRP also collects baseline data to be used to gauge the effectiveness of future actions once implemented. These baseline data are maintained in the Oak Ridge Environmental Information System and will be reported in future RERs, as necessary, once the respective actions are completed. However, when insufficient data exist to assess the impact of the RAs, e.g., when the RA was only recently completed, a preliminary evaluation is made of early indicators of effectiveness at the watershed scale, such as contaminant trends at surface water integration points (IPs). Long-term stewardship (LTS) information used in this report is collected, compiled, and tracked by the WRRP in conjunction with the Bechtel Jacobs Company LLC (BJC) Surveillance and Maintenance (S&M) program, the BJC Radiation Protection Organization at East Tennessee Technology Park (ETTP), and the ETTP Environmental Compliance Program. Additionally, documentation verifying the implementation of administrative land use controls (LUCs) [i.e., property record restrictions, property record notices, zoning notices, and excavation/penetration permit (EPP) program] is also obtained from many sources throughout the fiscal year (FY), including County Register of Deeds offices for property record restrictions and property record notices, City Planning Commission for zoning notices, and BJC project engineers for EPP program verification. Copies of this documentation are obtained by the WRRP and maintained with the project RER files.

  2. Management information systems software evaluation

    SciTech Connect (OSTI)

    Al-Tunisi, N.; Ghazzawi, A.; Gruyaert, F.; Clarke, D. [Saudi Aramco, Dhahran (Saudi Arabia). Process and Control Systems Dept.

    1995-11-01T23:59:59.000Z

    In November 1993, Saudi Aramco management endorsed a proposal to coordinate the development of the Management Information Systems (MISs) of four concurrent projects for its facilities Controls Modernization Program. The affected projects were the Ras Tanura Refinery Upgrade Project, the Abqaiq Plant Controls Modernization and the Shedgum and Uthmaniyah Gas plants Control Upgrade Projects. All of these projects had a significant requirement of MISs in their scope. Under the leadership of the Process and Control Systems Department, and MIS Coordination Team was formed with representatives of several departments. An MIS Applications Evaluation procedure was developed based on the Kepner Tregoe Decisions Analysis Process and general questionnaires were sent to over a hundred potential Vendors. The applications were divided into several categories, such as: Data Capture and Historization, Human User Interface, Trending, Reporting, Graphic Displays, Data Reconciliation, Statistical Analysis, Expert Systems, Maintenance Applications, Document Management and Operations Planning and Scheduling. For each of the MIS Application areas, detailed follow-up questionnaires were used to short list the candidate products. In May and June 1994, selected Vendors were invited to Saudi Arabia for an Exhibition which was open to all Saudi Aramco employees. In conjunction with this, the Vendors were subjected to a rigorous product testing exercise by independent teams of testers. The paper will describe the methods used and the lessons learned in this extensive software evaluation phase, which was a first for Saudi Aramco.

  3. Concanavalin A: A potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics

    SciTech Connect (OSTI)

    Li, Wen-wen; Yu, Jia-ying; Xu, Huai-long [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)] [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China); Bao, Jin-ku, E-mail: jinkubao@yahoo.com [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)] [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)

    2011-10-22T23:59:59.000Z

    Highlights: {yields} ConA induces cancer cell death targeting apoptosis and autophagy. {yields} ConA inhibits cancer cell angiogenesis. {yields} ConA is utilized in pre-clinical and clinical trials. -- Abstract: Concanavalin A (ConA), a Ca{sup 2+}/Mn{sup 2+}-dependent and mannose/glucose-binding legume lectin, has drawn a rising attention for its remarkable anti-proliferative and anti-tumor activities to a variety of cancer cells. ConA induces programmed cell death via mitochondria-mediated, P73-Foxo1a-Bim apoptosis and BNIP3-mediated mitochondrial autophagy. Through IKK-NF-{kappa}B-COX-2, SHP-2-MEK-1-ERK, and SHP-2-Ras-ERK anti-angiogenic pathways, ConA would inhibit cancer cell survival. In addition, ConA stimulates cell immunity and generates an immune memory, resisting to the same genotypic tumor. These biological findings shed light on new perspectives of ConA as a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis in pre-clinical or clinical trials for cancer therapeutics.

  4. Regulation of human hepatocellular carcinoma cells by Spred2 and correlative studies on its mechanism

    SciTech Connect (OSTI)

    Ma, Xiao-Ni [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Liu, Xiao-Yun [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China) [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Center for Disease Control and Prevention, Lanzhou Military Command, Lanzhou 730020 (China); Yang, Yue-Feng [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Xiao, Feng-Jun [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Li, Qing-Fang [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Yan, Jun [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Zhang, Qun-Wei; Wang, Li-Sheng [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Li, Xue-Yan, E-mail: llglixueyan@163.com [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Wang, Hua, E-mail: wanghua@bmi.ac.cn [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)

    2011-07-15T23:59:59.000Z

    Highlights: {yields} Hepatocellular carcinoma is inhibited by Spred2 through as yet unclear mechanisms. {yields} We studied the overexpression of Spred2 in cell line and murine tumor models of HCC. {yields} Spred2 inhibited cell proliferation and migration via attenuating ERK signaling. {yields} Spred2 overexpression induced apoptosis via caspase-3 and downregulated Mcl-1. {yields} A Spred2 knockdown markedly induced tumor growth in vivo. -- Abstract: Members of the Spred gene family are negative regulators of the Ras/Raf-1/ERK pathway, which has been associated with several features of the tumor malignancy. However, the effect of Spred genes on hepatocellular carcinoma (HCC) remains uninvestigated. In the present work, we analyzed the in vitro and in vivo effects of Spred2 expression on the hepatic carcinoma cell line, SMMC-7721. In addition to attenuated ERK activation, which inhibited the proliferation and migration of unstimulated and HGF-stimulated SMMC-7721 cells. Adenovirus-mediated Spred2 overexpression induced the activation of caspase-3 and apoptosis, as well as reduced the expression level of Mcl-1. Most importantly, the knockdown of Spred2 markedly enhanced tumor growth in vivo. In conclusion, these results suggest that Spred2 could qualify as a potential therapeutic target in HCC.

  5. Remedial action plan and site design for stabilization of the inactive uranium mill tailings site at Falls City, Texas. Remedial action selection report, attachment 2, geology report; attachment 3, groundwater hydrology report; and attachment 4, water resources protection strategy. Final report

    SciTech Connect (OSTI)

    NONE

    1992-09-01T23:59:59.000Z

    The uranium processing site near Falls City, Texas, was one of 24 inactive uranium mill sites designated to be remediated by the U.S. Department of Energy (DOE) under Title I of the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). The UMTRCA requires that the U.S. Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the U.S. Environmental Protection Agency (EPA). The RAP, which includes this summary remedial action selection report (RAS), serves a two-fold purpose. First, it describes the activities proposed by the DOE to accomplish long-term stabilization and control of the residual radioactive materials at the inactive uranium processing site near Falls City, Texas. Second, this document and the remainder of the RAP, upon concurrence and execution by the DOE, the State of Texas, and the NRC, becomes Appendix B of the Cooperative Agreement between the DOE and the State of Texas.

  6. LNG fleet increases in size and capabilities

    SciTech Connect (OSTI)

    Linser, H.J. Jr.; Drudy, M.J.; Endrizzi, F.; Urbanelli, A.A. [Mobil Shipping and Transportation, Fairfax, VA (United States)

    1997-06-02T23:59:59.000Z

    The LNG fleet as of early 1997 consisted of 99 vessels with total cargo capacity of 10.7 million cu m, equivalent to approximately 4.5 million tons. One of the newest additions to the fleet, the 137,000-cu m tanker Al Zubarah, is five times the size of the original commercial vessel Methane Princess. Al Zubarah`s first loading of more than 60,000 tons occurred in December 1996 for deliver to Japanese buyers from the newly commissioned Qatargas LNG plant at Ras Laffan. That size cargo contains enough clean-burning energy to heat 60,000 homes in Japan for 1 month. Measuring nearly 1,000 ft long, the tanker is among the largest in the industry fleet and joined 70 other vessels of more than 100,000 cu m. Most LNG tankers built since 1975 have been larger-capacity vessels. The paper discusses LNG shipping requirements, containment systems, vessel design, propulsion, construction, operations and maintenance, and the future for larger vessels.

  7. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Carl; Bernasek, Stephen L.; Abelev, Esta

    2010-02-19T23:59:59.000Z

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  8. Considerations for Contractile Electroactive Polymeric Materials and Actuators

    SciTech Connect (OSTI)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Charles; Bernasek, Stephen L.; Abelev, Esta

    2009-06-16T23:59:59.000Z

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  9. Increasing fault resiliency in a message-passing environment.

    SciTech Connect (OSTI)

    Stearley, Jon R.; Riesen, Rolf E.; Laros, James H., III; Ferreira, Kurt Brian; Pedretti, Kevin Thomas Tauke; Oldfield, Ron A.; Kordenbrock, Todd (Hewlett-Packard Company); Brightwell, Ronald Brian

    2009-10-01T23:59:59.000Z

    Petaflops systems will have tens to hundreds of thousands of compute nodes which increases the likelihood of faults. Applications use checkpoint/restart to recover from these faults, but even under ideal conditions, applications running on more than 30,000 nodes will likely spend more than half of their total run time saving checkpoints, restarting, and redoing work that was lost. We created a library that performs redundant computations on additional nodes allocated to the application. An active node and its redundant partner form a node bundle which will only fail, and cause an application restart, when both nodes in the bundle fail. The goal of this library is to learn whether this can be done entirely at the user level, what requirements this library places on a Reliability, Availability, and Serviceability (RAS) system, and what its impact on performance and run time is. We find that our redundant MPI layer library imposes a relatively modest performance penalty for applications, but that it greatly reduces the number of applications interrupts. This reduction in interrupts leads to huge savings in restart and rework time. For large-scale applications the savings compensate for the performance loss and the additional nodes required for redundant computations.

  10. Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Falls City, Texas. Remedial action selection report: Attachment 2, Geology report; Attachment 3, Groundwater hydrology report; Attachment 4, Water resources protection strategy: Final report

    SciTech Connect (OSTI)

    Chernoff, A.R. [USDOE Albuquerque Field Office, NM (United States). Uranium Mill Tailings Remedial Action Project Office; Lacker, D.K. [Texas State Dept. of Health, Austin, TX (United States). Bureau of Radiation Control

    1992-09-01T23:59:59.000Z

    The uranium processing site near Falls City, Texas, was one of 24 inactive uranium mill sites designated to be remediated by the US Department of Energy (DOE) under Title I of the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). The UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). The RAP, which includes this summary remedial action selection report (RAS), serves a two-fold purpose. First, it describes the activities proposed by the DOE to accomplish long-term stabilization and control of the residual radioactive materials at the inactive uranium processing site near Falls City, Texas. Second, this document and the remainder of the RAP, upon concurrence and execution by the DOE, the State of Texas, and the NRC, becomes Appendix B of the Cooperative Agreement between the DOE and the State of Texas.

  11. Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Falls City, Texas. [Uranium Mill Tailings Remedial Action (UMTRA) Project

    SciTech Connect (OSTI)

    Chernoff, A.R. (USDOE Albuquerque Field Office, NM (United States). Uranium Mill Tailings Remedial Action Project Office); Lacker, D.K. (Texas State Dept. of Health, Austin, TX (United States). Bureau of Radiation Control)

    1992-09-01T23:59:59.000Z

    The uranium processing site near Falls City, Texas, was one of 24 inactive uranium mill sites designated to be remediated by the US Department of Energy (DOE) under Title I of the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). The UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE's remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). The RAP, which includes this summary remedial action selection report (RAS), serves a two-fold purpose. First, it describes the activities proposed by the DOE to accomplish long-term stabilization and control of the residual radioactive materials at the inactive uranium processing site near Falls City, Texas. Second, this document and the remainder of the RAP, upon concurrence and execution by the DOE, the State of Texas, and the NRC, becomes Appendix B of the Cooperative Agreement between the DOE and the State of Texas.

  12. Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Durango, Colorado: Remedial action selection report. Revised final report

    SciTech Connect (OSTI)

    Not Available

    1991-12-01T23:59:59.000Z

    The uranium mill tailings site near Durango, Colorado, was one of 24 inactive uranium mill sites designated to be remediated by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s Remedial Action Plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which has been developed to serve a two-fold purpose. First, it describes the activities that have been conducted by the DOE to accomplish remediation and long-term stabilization and control of the radioactive materials at the inactive uranium mill processing site near Durango, Colorado. Secondly, this document and the rest of the RAP, upon concurrence and execution by the DOE, the State of Colorado, and the NRC, become Appendix B of the Cooperative Agreement between the DOE and the State of Colorado.

  13. Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Durango, Colorado: Remedial action selection report

    SciTech Connect (OSTI)

    Not Available

    1991-12-01T23:59:59.000Z

    The uranium mill tailings site near Durango, Colorado, was one of 24 inactive uranium mill sites designated to be remediated by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE's Remedial Action Plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which has been developed to serve a two-fold purpose. First, it describes the activities that have been conducted by the DOE to accomplish remediation and long-term stabilization and control of the radioactive materials at the inactive uranium mill processing site near Durango, Colorado. Secondly, this document and the rest of the RAP, upon concurrence and execution by the DOE, the State of Colorado, and the NRC, become Appendix B of the Cooperative Agreement between the DOE and the State of Colorado.

  14. Optical and infrared emission of H II complexes as a clue to PAHs lifecycle

    E-Print Network [OSTI]

    Khramtsova, M S; Lozinskaya, T A; Egorov, O V

    2014-01-01T23:59:59.000Z

    We present an analysis of optical spectroscopy and infrared aperture photometry of more than 100 H II complexes in nine galaxies. Spectra obtained with the 6-m telescope of SAO RAS are used along with archival data from Spitzer and several ground-based telescopes to infer a strength of polycyclic aromatic hydrocarbon (PAH) emission, age, properties of the UV radiation field, and metallicity of studied H II complexes. Physical properties (age, radiation field parameters, metallicity) are related to the $F_{8}/F_{24}$ ratio used as a proxy for the PAH abundance in order to reveal factors that may influence the PAH evolution in H II complexes. The well-known correlation between the $F_{8}/F_{24}$ ratio and metallicity is confirmed in the studied complexes. The infrared flux ratio also correlates with the [O III]$\\lambda 5007/\\mathrm{H\\beta}$ ratio which is often considered as an indicator of the radiation field hardness, but this correlation seems to be a mere reflection of a correlation between [O III]$\\lambda ...

  15. Remedial Action Assessment System (RAAS): Evaluation of selected feasibility studies of CERCLA (Comprehensive Environmental Response, Compensation, and Liability Act) hazardous waste sites

    SciTech Connect (OSTI)

    Whelan, G. (Pacific Northwest Lab., Richland, WA (USA)); Hartz, K.E.; Hilliard, N.D. (Beck (R.W.) and Associates, Seattle, WA (USA))

    1990-04-01T23:59:59.000Z

    Congress and the public have mandated much closer scrutiny of the management of chemically hazardous and radioactive mixed wastes. Legislative language, regulatory intent, and prudent technical judgment, call for using scientifically based studies to assess current conditions and to evaluate and select costeffective strategies for mitigating unacceptable situations. The NCP requires that a Remedial Investigation (RI) and a Feasibility Study (FS) be conducted at each site targeted for remedial response action. The goal of the RI is to obtain the site data needed so that the potential impacts on public health or welfare or on the environment can be evaluated and so that the remedial alternatives can be identified and selected. The goal of the FS is to identify and evaluate alternative remedial actions (including a no-action alternative) in terms of their cost, effectiveness, and engineering feasibility. The NCP also requires the analysis of impacts on public health and welfare and on the environment; this analysis is the endangerment assessment (EA). In summary, the RI, EA, and FS processes require assessment of the contamination at a site, of the potential impacts in public health or the environment from that contamination, and of alternative RAs that could address potential impacts to the environment. 35 refs., 7 figs., 1 tab.

  16. Rap G protein signal in normal and disordered lymphohematopoiesis

    SciTech Connect (OSTI)

    Minato, Nagahiro, E-mail: minato@imm.med.kyoto-u.ac.jp

    2013-09-10T23:59:59.000Z

    Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins including guanine nucleotide exchange factors and GTPase-activating proteins. Beyond cell biological studies, increasing attempts have been made in the past decade to define the roles of Rap signal in specific functions of normal tissue systems as well as in cancer. In the immune and hematopoietic systems, Rap signal plays crucial roles in the development and function of essentially all lineages of lymphocytes and hematopoietic cells, and importantly, deregulated Rap signal may lead to unique pathological conditions depending on the affected cell types, including various types of leukemia and autoimmunity. The phenotypical studies have unveiled novel, even unexpected functional aspects of Rap signal in cells from a variety of tissues, providing potentially important clues for controlling human diseases, including malignancy.

  17. Fiscal Year 2008 Phased Construction Completion Report for EU Z2-33 in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2008-09-11T23:59:59.000Z

    The Record of Decision for Soil, Buried Waste, and Subsurface Structure Actions in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2161&D2) (Zone 2 ROD) acknowledged that most of the 800 acres in Zone 2 were contaminated, but that sufficient data to confirm the levels of contamination were lacking. The Zone 2 ROD further specified that a sampling strategy for filling the data gaps would be developed. The Remedial Design Report/Remedial Action Work Plan for Zone 2 Soils, Slabs, and Subsurface Structures, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2224&D3) (Zone 2 RDR/RAWP) defined the sampling strategy as the Dynamic Verification Strategy (DVS), generally following the approach used for characterization of the Zone 1 exposure units (EUs). The Zone 2 ROD divided the Zone 2 area into seven geographic areas and 44 EUs. To facilitate the data quality objectives (DQOs) of the DVS process, the Zone 2 RDR/RAWP regrouped the 44 EUs into 12 DQO scoping EU groups. These groups facilitated the DQO process by placing similar facilities and their support facilities together and allowing identification of data gaps. The EU groups were no longer pertinent after DQO planning was completed and characterization was conducted as areas became accessible. As the opportunity to complete characterization became available, the planned DVS program and remedial actions (RAs) were completed for EU Z2-33. Remedial action was also performed at two additional areas in adjacent EU Z2-42 because of their close proximity and similar nature to a small surface soil RA in EU Z2-33. Remedial actions for building slabs performed in EU Z2-33 during fiscal year (FY) 2007 were reported in the Fiscal Year 2007 Phased Construction Completion Report for the Zone 2 Soils, Slabs, and Subsurface Structures at East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2723&D1). Recommended RAs for EU Z2-42 were described in the Fiscal Year 2006 Phased Construction Completion Report for the Zone 2 Soils, Slabs, and Subsurface Structures at East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2317&D2). Remedial actions performed in the Balance of Site (BOS) Laboratory Area of EU Z2-33 and two small areas in EU Z2-42 are described in Sects. 5 through 10 of this Phased Construction Completion Report (PCCR). The purpose of this PCCR is to address the following: (1) Document DVS characterization results for EU Z2-33; (2) Describe and document the risk evaluation and determine if the EU meets the Zone 2 ROD requirements for unrestricted industrial use to 10 ft bgs; (3) Identify additional areas not defined in the Zone 2 ROD that require remediation based on the DVS evaluation results; and (4) Describe RAs performed in the EU Z2-33 BOS Laboratory Area and two small areas in EU Z2-42. Approximately 18 acres in EU Z2-33 are addressed in this PCCR. Based on the results of the DVS evaluation and RAs performed, all 18 acres are recommended for unrestricted industrial use to 10 ft bgs. Three Federal Facility Agreement sites are addressed and recommended for no further action within this acreage, including: (1) K-1004-L Recirculating Cooling Water Lines Leak Sites; (2) K-1044 Heavy Equipment Repair Shop; and (3) K-1015-A Laundry Pit. Remedial actions for EU Z2-33 were developed in response to DVS characterization results described in the EU Z2-33 Technical Memorandum (Appendix A) and to support reindustrialization of the East Tennessee Technology Park as a commercial industrial park. Remediation criteria were designed for the protection of a future industrial worker who normally would not have the potential for exposure to soil below 10ft bgs. Accordingly, the Zone 2 ROD required land use controls to prevent disturbance of soils below 10 ft deep and to restrict future land use to industrial/commercial activities. In response to stakeholder comments, the U.S. Department of Energy agreed to re-evaluate the need for such land use restrictions. This document includes a screening evaluation to determine the likel

  18. Structure and kinematics of candidate double-barred galaxies

    E-Print Network [OSTI]

    Moiseev, A V; Chavushyan, V H

    2003-01-01T23:59:59.000Z

    Results of optical and NIR spectral and photometric observations of a sample of candidate double-barred galaxies are presented. Velocity fields and velocity dispersion maps of stars and ionized gas, continuum and emission-line images were constructed from integral-field spectroscopy observations carried out at the 6m telescope (BTA) of SAO RAS, with the MPFS spectrograph and the scanning Fabry-Perot Interferometer. NGC2681 was also observed with long-slit spectrograph of the BTA. Optical and NIR images were obtained at the BTA and at the 2.1m telescope (OAN, M\\'exico). High-resolution images were retrieved from the HST data archive. Morphological and kinematic features of all 13 sample objects are described in detail. Attention is focused on the interpretation of observed non-circular motions of gas and stars in circumnuclear (one kiloparsec- scale) regions. We have shown first of all that these motions are caused by a gravitational potential of large-scale bar. NGC3368 and NGC3786 have nuclear bars only, the...

  19. Molecular genetics in affective illness

    SciTech Connect (OSTI)

    Mendlewicz, J.; Sevy, S.; Mendelbaum, K. (Erasme Univ. Hospital, Brussels (Belgium))

    1993-01-01T23:59:59.000Z

    Genetic transmission in manic depressive illness (MDI) has been explored in twins, adoption, association, and linkage studies. The X-linked transmission hypothesis has been tested by using several markers on chromosome X: Xg blood group, color blindness, glucose-6-phosphate dehydrogenase (G6PD), factor IX (hemophilia B), and DNA probes such as DXS15, DXS52, F8C, ST14. The hypothesis of autosomal transmission has been tested by association studies with the O blood group located on chromosome 9, as well as linkage studies on chromosome 6 with the Human Leucocyte Antigens (HLA) haplotypes and on Chromosome 11 with DNA markers for the following genes: D2 dopamine receptor, tyrosinase, C-Harvey-Ras-A (HRAS) oncogene, insuline (ins), and tyrosine hydroxylase (TH). Although linkage studies support the hypothesis of a major locus for the transmission of MDI in the Xq27-28 region, several factors are limiting the results, and are discussed in the present review. 105 refs., 1 fig., 2 tabs.

  20. Time-dependent restricted-active-space self-consistent-field singles method for many-electron dynamics

    SciTech Connect (OSTI)

    Miyagi, Haruhide; Bojer Madsen, Lars [Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C (Denmark)] [Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C (Denmark)

    2014-04-28T23:59:59.000Z

    The time-dependent restricted-active-space self-consistent-field singles (TD-RASSCF-S) method is presented for investigating TD many-electron dynamics in atoms and molecules. Adopting the SCF notion from the muticonfigurational TD Hartree-Fock (MCTDHF) method and the RAS scheme (single-orbital excitation concept) from the TD configuration-interaction singles (TDCIS) method, the TD-RASSCF-S method can be regarded as a hybrid of them. We prove that, for closed-shell N{sub e}-electron systems, the TD-RASSCF-S wave function can be fully converged using only N{sub e}/2 + 1 ? M ? N{sub e} spatial orbitals. Importantly, based on the TD variational principle, the converged TD-RASSCF-S wave function with M = N{sub e} is more accurate than the TDCIS wave function. The accuracy of the TD-RASSCF-S approach over the TDCIS is illustrated by the calculation of high-order harmonic generation spectra for one-dimensional models of atomic helium, beryllium, and carbon in an intense laser pulse. The electronic dynamics during the process is investigated by analyzing the behavior of electron density and orbitals. The TD-RASSCF-S method is accurate, numerically tractable, and applicable for large systems beyond the capability of the MCTDHF method.

  1. Remedial action plan and site design for stabilization of the inactive uranium processing site at Naturita, Colorado. Remedial action selection report, Attachment 2, Geology report: Preliminary final

    SciTech Connect (OSTI)

    Not Available

    1993-08-01T23:59:59.000Z

    The uranium processing site near Naturita, Colorado, is one of 24 inactive uranium mill sites designated to be cleaned up by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA), Public Law 95-604. Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which serves two purposes. First, it describes the activities that are proposed by the DOE to accomplish remediation and long-term stabilization and control of the radioactive materials at the inactive uranium processing site near Naturita, Colorado. Second, this document and the rest of the RAP, upon concurrence and execution by the DOE, the state of Colorado, and the NRC, become Appendix B of the cooperative agreement between the DOE and the State of Colorado.

  2. 2011 Remediation Effectiveness Report for the U.S. Department of Energy Oak Ridge Reservation, Oak Ridge, Tennessee - Data and Evaluations

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2011-03-01T23:59:59.000Z

    Under the requirements of the Oak Ridge Reservation (ORR) Federal Facility Agreement (FFA) established between the U.S. Department of Energy (DOE), the U.S. Environmental Protection Agency, (EPA) and the Tennessee Department of Environment and Conservation (TDEC) in 1992, all environmental restoration activities on the ORR are performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Since the 1990s, the environmental restoration activities have experienced a gradual shift from characterization to remediation. As this has occurred, it has been determined that the assessment of the individual and cumulative performance of all ORR CERCLA remedial actions (RAs) is most effectively tracked in a single document. The Remediation Effectiveness Report (RER) is an FFA document intended to collate all ORR CERCLA decision requirements, compare pre- and post-remediation conditions at CERCLA sites, and present the results of any required post-decision remediation effectiveness monitoring. First issued in 1997, the RER has been reissued annually to update the performance histories of completed actions and to add descriptions of new CERCLA actions. Monitoring information used in the 2011 RER to assess remedy performance was collected and/or compiled by DOE's Water Resources Restoration Program (WRRP). Only data used to assess performance of completed actions are provided. In addition to collecting CERCLA performance assessment data, the WRRP also collects baseline data to be used to gauge the effectiveness of future actions once implemented. These baseline data are maintained in the Oak Ridge Environmental Information System and will be reported in future RERs, as necessary, once the respective actions are completed. However, when insufficient data exist to assess the impact of the RAs, e.g., when the RA was only recently completed, a preliminary evaluation is made of early indicators of effectiveness at the watershed scale, such as contaminant trends at surface water integration points (IFs). Long-term stewardship (LTS) information used in this report is collected, compiled, and tracked by the WRRP in conjunction with the Bechtel Jacobs Company LLC (BJC) Surveillance and Maintenance (S&M) program, the BJC Radiation Protection Organization at East Tennessee Technology Park (ETTP), ETTP Environmental Compliance Program, B&W Y-12 Liquid Waste Treatment Operations, and UT Battelle Facilities Management Division. Additionally, documentation verifying the implementation of administrative land use controls (LUCs) [i.e., property record restrictions, property record notices, zoning notices, and excavation/penetration permit (EPP) program] is also obtained from many sources throughout the fiscal year (FY), including County Register of Deeds offices for property record restrictions and property record notices, City Planning Commission for zoning notices, and BJC project engineers for EPP program verification. Copies of this documentation are obtained by the WRRP and maintained with the project RER files.

  3. HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment

    SciTech Connect (OSTI)

    Weigelt, Britta; Lo, Alvin T; Park, Catherine C; Gray, Joe W; Bissell, Mina J

    2009-07-27T23:59:59.000Z

    Development of effective and durable breast cancer treatment strategies requires a mechanistic understanding of the influence of the microenvironment on response. Previous work has shown that cellular signaling pathways and cell morphology are dramatically influenced by three-dimensional (3D) cultures as opposed to traditional two-dimensional (2D) monolayers. Here, we compared 2D and 3D culture models to determine the impact of 3D architecture and extracellular matrix (ECM) on HER2 signaling and on the response of HER2-amplified breast cancer cell lines to the HER2-targeting agents Trastuzumab, Pertuzumab and Lapatinib. We show that the response of the HER2-amplified AU565, SKBR3 and HCC1569 cells to these anti-HER2 agents was highly dependent on whether the cells were cultured in 2D monolayer or 3D laminin-rich ECM gels. Inhibition of {beta}1 integrin, a major cell-ECM receptor subunit, significantly increased the sensitivity of the HER2-amplified breast cancer cell lines to the humanized monoclonal antibodies Trastuzumab and Pertuzumab when grown in a 3D environment. Finally, in the absence of inhibitors, 3D cultures had substantial impact on HER2 downstream signaling and induced a switch between PI3K-AKT- and RAS-MAPKpathway activation in all cell lines studied, including cells lacking HER2 amplification and overexpression. Our data provide direct evidence that breast cancer cells are able to rapidly adapt to different environments and signaling cues by activating alternative pathways that regulate proliferation and cell survival, events that may play a significant role in the acquisition of resistance to targeted therapies.

  4. Eckol suppresses maintenance of stemness and malignancies in glioma stem-like cells

    SciTech Connect (OSTI)

    Hyun, Kyung-Hwan; Yoon, Chang-Hwan; Kim, Rae-Kwon; Lim, Eun-Jung [Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul 133-791 (Korea, Republic of); An, Sungkwan [Functional Genoproteome Research Centre, Konkuk University, Seoul 143-701 (Korea, Republic of); Park, Myung-Jin [Division of Radiation Cancer Biology, Korea Institute of Radiological and Medical Sciences, Seoul 139-706 (Korea, Republic of); Hyun, Jin-Won [College of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756 (Korea, Republic of); Suh, Yongjoon [Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul 133-791 (Korea, Republic of); Kim, Min-Jung, E-mail: kimmj74@hanyang.ac.kr [Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul 133-791 (Korea, Republic of); Lee, Su-Jae, E-mail: sj0420@hanyang.ac.kr [Department of Chemistry, Research Institute for Natural Sciences, Hanyang University, Seoul 133-791 (Korea, Republic of)

    2011-07-01T23:59:59.000Z

    A subpopulation of cancer cells with stem cell properties is responsible for tumor maintenance and progression, and may contribute to resistance to anticancer treatments. Thus, compounds that target cancer stem-like cells could be usefully applied to destroy cancer. In this study, we investigated the effect of Eckol, a phlorotannin compound, on stemness and malignancies in glioma stem-like cells. To determine whether Eckol targets glioma stem-like cells, we examined whether Eckol treatment could change the expression levels of glioma stem-like cell markers and self-renewal-related proteins as well as the sphere forming ability, and the sensitivity to anticancer treatments. Alterations in the malignant properties of sphere-derived cells by Eckol were also investigated by soft-agar colony forming assay, by xenograft assay in nude mice, and by cell invasion assay. Treatment of sphere-forming glioma cells with Eckol effectively decreased the sphere formation as well as the CD133{sup +} cell population. Eckol treatment suppressed expression of the glioma stem-like cell markers and the self-renewal-related proteins without cell death. Moreover, treatment of glioma stem-like cells with Eckol significantly attenuated anchorage-independent growth on soft agar and tumor formation in xenograft mice. Importantly, Eckol treatment effectively reduced the resistance of glioma stem-like cells to ionizing radiation and temozolomide. Treatment of glioma stem-like cells with Eckol markedly blocked both phosphoinositide 3-kinase-Akt and Ras-Raf-1-Erk signaling pathways. These results indicate that the natural phlorotannin Eckol suppresses stemness and malignancies in glioma stem-like cells, and thereby makes glioma stem-like cells more sensitive to anticancer treatments, providing novel therapeutic strategies targeting specifically cancer stem-like cells.

  5. TEC protein tyrosine kinase is involved in the Erk signaling pathway induced by HGF

    SciTech Connect (OSTI)

    Li, Feifei; Jiang, Yinan [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China)] [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China); Zheng, Qiping [Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612 (United States)] [Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612 (United States); Yang, Xiaoming [Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 (China)] [Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 (China); Wang, Siying, E-mail: sywang@ahmu.edu.cn [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China)] [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China)

    2011-01-07T23:59:59.000Z

    Research highlights: {yields} TEC is rapidly tyrosine-phosphorylated and activated by HGF-stimulation in vivo or after partial hepatectomy in mice. {yields} TEC enhances the activity of Elk and serum response element (SRE) in HGF signaling pathway in hepatocyte. {yields} TEC promotes hepatocyte proliferation through the Erk-MAPK pathway. -- Abstract: Background/aims: TEC, a member of the TEC family of non-receptor type protein tyrosine kinases, has recently been suggested to play a role in hepatocyte proliferation and liver regeneration. This study aims to investigate the putative mechanisms of TEC kinase regulation of hepatocyte differentiation, i.e. to explore which signaling pathway TEC is involved in, and how TEC is activated in hepatocyte after hepatectomy and hepatocyte growth factor (HGF) stimulation. Methods: We performed immunoprecipitation (IP) and immunoblotting (IB) to examine TEC tyrosine phosphorylation after partial hepatectomy in mice and HGF stimulation in WB F-344 hepatic cells. The TEC kinase activity was determined by in vitro kinase assay. Reporter gene assay, antisense oligonucleotide and TEC dominant negative mutant (TEC{sup KM}) were used to examine the possible signaling pathways in which TEC is involved. The cell proliferation rate was evaluated by {sup 3}H-TdR incorporation. Results: TEC phosphorylation and kinase activity were increased in 1 h after hepatectomy or HGF treatment. TEC enhanced the activity of Elk and serum response element (SRE). Inhibition of MEK1 suppressed TEC phosphorylation. Blocking TEC activity dramatically decreased the activation of Erk. Reduced TEC kinase activity also suppressed the proliferation of WB F-344 cells. These results suggest TEC is involved in the Ras-MAPK pathway and acts between MEK1 and Erk. Conclusions: TEC promotes hepatocyte proliferation and regeneration and is involved in HGF-induced Erk signaling pathway.

  6. KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1

    SciTech Connect (OSTI)

    Goettel, Jeremy A. [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States)] [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Liang, Dongchun [Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States)] [Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Hilliard, Valda C.; Edelblum, Karen L.; Broadus, Matthew R. [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States)] [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Gould, Kathleen L. [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States) [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Hanks, Steven K. [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States)] [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Polk, D. Brent, E-mail: dbpolk@chla.usc.edu [Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States); Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine, Nashville, TN 37232 (United States)

    2011-02-15T23:59:59.000Z

    The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway is a highly conserved signaling pathway that regulates diverse cellular processes including differentiation, proliferation, and survival. Kinase suppressor of Ras-1 (KSR1) binds each of the three ERK cascade components to facilitate pathway activation. Even though KSR1 contains a C-terminal kinase domain, evidence supporting the catalytic function of KSR1 remains controversial. In this study, we produced recombinant wild-type or kinase-inactive (D683A/D700A) KSR1 proteins in Escherichia coli to test the hypothesis that KSR1 is a functional protein kinase. Recombinant wild-type KSR1, but not recombinant kinase-inactive KSR1, underwent autophosphorylation on serine residue(s), phosphorylated myelin basic protein (MBP) as a generic substrate, and phosphorylated recombinant kinase-inactive MAPK/ERK kinase-1 (MEK1). Furthermore, FLAG immunoprecipitates from KSR1{sup -/-} colon epithelial cells stably expressing FLAG-tagged wild-type KSR1 (+KSR1), but not vector (+vector) or FLAG-tagged kinase-inactive KSR1 (+D683A/D700A), were able to phosphorylate kinase-inactive MEK1. Since TNF activates the ERK pathway in colon epithelial cells, we tested the biological effects of KSR1 in the survival response downstream of TNF. We found that +vector and +D683A/D700A cells underwent apoptosis when treated with TNF, whereas +KSR1 cells were resistant. However, +KSR1 cells were sensitized to TNF-induced cell loss in the absence of MEK kinase activity. These data provide clear evidence that KSR1 is a functional protein kinase, MEK1 is an in vitro substrate of KSR1, and the catalytic activities of both proteins are required for eliciting cell survival responses downstream of TNF.

  7. Inhibition of the ERK phosphorylation plays a role in terbinafine-induced p21 up-regulation and DNA synthesis inhibition in human vascular endothelial cells

    SciTech Connect (OSTI)

    Ho, P.-Y. [Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei, Taiwan (China); Hsu, S.-P. [Institute of Physiology, College of Medicine, National Taiwan University, Taiwan (China); Liang, Y.-C. [Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan (China); Kuo, M.-L. [Institute of Toxicology, College of Medicine, National Taiwan University, Taiwan (China); Ho, Y.-S. [Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan (China); Lee, W.-S. [Graduate Institute of Neuroscience, Taipei Medical University, Taipei, Taiwan (China); Graduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (China); Department of Physiology, Medical College, Taipei Medical University, Taipei, Taiwan (China)], E-mail: wslee@tmu.edu.tw

    2008-05-15T23:59:59.000Z

    Previously, we showed that terbinafine (TB) induces cell-cycle arrest in cultured human umbilical vein endothelial cells (HUVEC) through an up-regulation of the p21 protein. The aim of this study is to delineate the molecular mechanisms underlying TB-induced increase of p21 protein. RT-PCR analysis demonstrated that the mRNA levels of p21 and p53 were increased in the TB-treated HUVEC. The p21 promoter activity was also increased by TB treatment. Transfection of HUVEC with p53 dominant negative (DN) abolished the TB-induced increases of p21 promoter activity and protein level, suggesting that the TB-induced increase of p21 is p53-dependent. Western blot analysis demonstrated that TB decreased the levels of phosphorylated extracellular signal-regulated kinase (ERK). Over-expression of mitogen-activated protein kinase (MEK)-1, the immediate upstream activator kinase of ERK, abolished the TB-induced increases of p21 and p53 protein and decrease of thymidine incorporation. The ERK inhibitor (PD98059) enhanced the TB-induced inhibition of thymidine incorporation into HUVEC. Taken together, these data suggest that the decrease of ERK activity plays a role in the TB-induced up-regulation of p21 in HUVEC. On the other hand, pretreatment of the cells with geranylgeraniol (GGOH), farnesol (FOH), or Ras inhibitor peptide did not affect the TB-induced decrease of thymidine incorporation. Taken together, our results suggest that TB might cause a decrease of MEK, which in turn up-regulates p53 through the inhibition of ERK phosphorylation, and finally causes an increase of p21 expression and cell-cycle arrest.

  8. Lysophosphatidic Acid-induced ERK Activation and Chemotaxis in MC3T3-E1 Preosteoblasts are Independent of EGF Receptor Transactivation

    SciTech Connect (OSTI)

    Karagiosis, Sue A.; Chrisler, William B.; Bollinger, Nikki; Karin, Norman J.

    2009-06-01T23:59:59.000Z

    Growing evidence indicates that bone-forming osteoblasts and their progenitors are target cells for the lipid growth factor lysophosphatidic acid (LPA) which is produced by degranulating platelets at sites of injury. LPA is a potent inducer of bone cell migration, proliferation and survival in vitro and an attractive candidate to facilitate preosteoblast chemotaxis during skeletal regeneration in vivo, but the intracellular signaling pathways mediating the effects of this lipid on bone cells are not defined. In this study we measured the ability of LPA to stimulate extracellular signal-related kinase (ERK1/2) in MC3T3-E1 preosteoblastic cells and determined the contribution of this pathway to LPA-stimulated chemotaxis. LPA-treated cells exhibited a bimodal activation of ERK1/2 with maximal phosphorylation at 5 and 60 minutes. The kinetics of ERK1/2 phosphorylation were not coupled to Ras activation or LPA-induced elevations in cytosolic Ca2+. While LPA is coupled to the transactivation of the EGF receptor in many cell types, LPA-stimulated ERK1/2 activation in MC3T3-E1 cells was unaffected by inhibition of EGF receptor function. ERK isoforms rapidly accumulated at nuclear sites in LPA-treated cells, a process that was blocked if ERK1/2 phosphorylation was prevented with the MEK1 inhibitor U0126. Blocking ERK1/2 phosphorylation with U0126 also diminished MC3T3-E1 cell migration and altered the normal disassembly of LPA-induced stress fibers, while the inhibition of EGF receptor function had no effect on LPA-coupled preosteoblast motility. Our results identify ERK1/2 activation as a mediatora mediator of LPA-stimulated MC3T3-E1 cell migration that may be relevant to preosteoblast motility during bone repair in vivo.

  9. Id-1 is induced in MDCK epithelial cells by activated Erk/MAPK pathway in response to expression of the Snail and E47 transcription factors

    SciTech Connect (OSTI)

    Jorda, Mireia [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Vinyals, Antonia [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Marazuela, Anna [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Cubillo, Eva [Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM) and Departamento de Bioquimica (UAM), Madrid (Spain); Olmeda, David [Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM) and Departamento de Bioquimica (UAM), Madrid (Spain); Valero, Eva [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Cano, Amparo [Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM) and Departamento de Bioquimica (UAM), Madrid (Spain); Fabra, Angels [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain)]. E-mail: afabra@idibell.org

    2007-07-01T23:59:59.000Z

    Id-1, a member of the helix-loop-helix transcription factor family has been shown to be involved in cell proliferation, angiogenesis and invasion of many types of human cancers. We have previously shown that stable expression of E47 and Snail repressors of the E-cadherin promoter in MDCK epithelial cell line triggers epithelial mesenchymal transition (EMT) concomitantly with changes in gene expression. We show here that both factors activate the Id-1 gene promoter and induce Id-1 mRNA and protein. The upregulation of the Id-1 gene occurs through the transactivation of the promoter by the Erk/MAPK signaling pathway. Moreover, oncogenic Ras is also able to activate Id-1 promoter in MDCK cells in the absence of both E47 and Snail transcription factors. Several transcriptionally active regulatory elements have been identified in the proximal promoter, including AP-1, Sp1 and four putative E-boxes. By EMSA, we only detected an increased binding to Sp1 and AP-1 elements in E47- and Snail-expressing cells. Binding is affected by the treatment of cells with PD 98059 MEK inhibitor, suggesting that MAPK/Erk contributes to the recruitment or assembly of proteins to Id-1 promoter. Small interfering RNA directed against Sp1 reduced Id-1 expression and the upregulation of the promoter, indicating that Sp1 is required for Id-1 induction in E47- and Snail-expressing cells. Our results provide new insights into how some target genes are activated during and/or as a consequence of the EMT triggered by both E47 and Snail transcription factors.

  10. US DOE-EM On-Site Disposal Cell Working Group - Fostering Communication On Performance Assessment Challenges

    SciTech Connect (OSTI)

    Seitz, Roger R. [Savannah River Site (SRS), Aiken, SC (United States); Suttora, Linda C. [U.S. Department of Energy, Office of Site Restoration, Germantown, MD (United States); Phifer, Mark [Savannah River Site (SRS), Aiken, SC (United States)

    2014-03-01T23:59:59.000Z

    On-site disposal cells are in use and being considered at several U.S. Department of Energy (USDOE) sites as the final disposition for large amounts of waste associated with cleanup of contaminated areas and facilities. These facilities are typically developed with regulatory oversight from States and/or the US Environmental Protection Agency (USEPA) in addition to USDOE. The facilities are developed to meet design standards for disposal of hazardous waste as well as the USDOE performance based standards for disposal of radioactive waste. The involvement of multiple and different regulators for facilities across separate sites has resulted in some differences in expectations for performance assessments and risk assessments (PA/RA) that are developed for the disposal facilities. The USDOE-EM Office of Site Restoration formed a working group to foster improved communication and sharing of information for personnel associated with these Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) disposal cells and work towards more consistent assumptions, as appropriate, for technical and policy considerations related to performance and risk assessments in support of a Record of Decision and Disposal Authorization Statement. The working group holds teleconferences, as needed, focusing on specific topics of interest. The topics addressed to date include an assessment of the assumptions used for performance assessments and risk assessments (PA/RAs) for on-site disposal cells, requirements and assumptions related to assessment of inadvertent intrusion, DOE Manual 435.1-1 requirements, and approaches for consideration of the long-term performance of liners and covers in the context of PAs. The working group has improved communication among the staff and oversight personnel responsible for onsite disposal cells and has provided a forum to identify and resolve common concerns.

  11. Unique Structural and Nucleotide Exchange Features of the Rho1 GTPase of Entamoeba histolytica

    SciTech Connect (OSTI)

    Bosch, Dustin E.; Wittchen, Erika S.; Qiu, Connie; Burridge, Keith; Siderovski, David P. (UNC)

    2012-08-10T23:59:59.000Z

    The single-celled human parasite Entamoeba histolytica possesses a dynamic actin cytoskeleton vital for its intestinal and systemic pathogenicity. The E. histolytica genome encodes several Rho family GTPases known to regulate cytoskeletal dynamics. EhRho1, the first family member identified, was reported to be insensitive to the Rho GTPase-specific Clostridium botulinum C3 exoenzyme, raising the possibility that it may be a misclassified Ras family member. Here, we report the crystal structures of EhRho1 in both active and inactive states. EhRho1 is activated by a conserved switch mechanism, but diverges from mammalian Rho GTPases in lacking a signature Rho insert helix. EhRho1 engages a homolog of mDia, EhFormin1, suggesting a role in mediating serum-stimulated actin reorganization and microtubule formation during mitosis. EhRho1, but not a constitutively active mutant, interacts with a newly identified EhRhoGDI in a prenylation-dependent manner. Furthermore, constitutively active EhRho1 induces actin stress fiber formation in mammalian fibroblasts, thereby identifying it as a functional Rho family GTPase. EhRho1 exhibits a fast rate of nucleotide exchange relative to mammalian Rho GTPases due to a distinctive switch one isoleucine residue reminiscent of the constitutively active F28L mutation in human Cdc42, which for the latter protein, is sufficient for cellular transformation. Nonconserved, nucleotide-interacting residues within EhRho1, revealed by the crystal structure models, were observed to contribute a moderating influence on fast spontaneous nucleotide exchange. Collectively, these observations indicate that EhRho1 is a bona fide member of the Rho GTPase family, albeit with unique structural and functional aspects compared with mammalian Rho GTPases.

  12. Structure and kinematics of candidate double-barred galaxies

    E-Print Network [OSTI]

    A. V. Moiseev; J. R. Valdes; V. H. Chavushyan

    2004-02-02T23:59:59.000Z

    Results of optical and NIR spectral and photometric observations of a sample of candidate double-barred galaxies are presented. Velocity fields and velocity dispersion maps of stars and ionized gas, continuum and emission-line images were constructed from integral-field spectroscopy observations carried out at the 6m telescope (BTA) of SAO RAS, with the MPFS spectrograph and the scanning Fabry-Perot Interferometer. NGC2681 was also observed with the long-slit spectrograph of the BTA. Optical and NIR images were obtained at the BTA and at the 2.1m telescope (OAN, M\\'exico). High-resolution images were retrieved from the HST data archive. Morphological and kinematic features of all 13 sample objects are described in detail. Attention is focused on the interpretation of observed non-circular motions of gas and stars in circumnuclear (one kiloparsec- scale) regions. We have shown first of all that these motions are caused by the gravitational potential of a large-scale bar. NGC3368 and NGC3786 have nuclear bars only, their isophotal twist at larger radii being connected with the bright spiral arms. Three cases of inner polar disks in our sample (NGC2681, NGC3368 and NGC5850) are considered. We found ionized-gas counter-rotation in the central kiloparsec of the lenticular galaxy NGC3945. Seven galaxies (NGC470, NGC2273, NGC2681, NGC3945, NGC5566, NGC5905, and NGC6951) have inner mini-disks nested in large-scale bars. Minispiral structures occur often in these nuclear disks. It is interesting that the majority of the observed, morphological and kinematical, features in the sample galaxies can be explained without the secondary bar hypothesis. Thus we suggest that a dynamically independent secondary bar is a rarer phenomenon than follows from isophotal analysis of the images only.

  13. 2,3,7,8-Tetrachlorodibenzo-p-dioxin causes increases in expression of c-erb-A and levels of protein-tyrosine kinases in selected tissues of responsive mouse strains

    SciTech Connect (OSTI)

    Bombick, D.W.; Jankun, J.; Tullis, K.; Matsumura, F. (Michigan State Univ., East Lansing (USA))

    1988-06-01T23:59:59.000Z

    2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) administered in vivo causes drastic reduction in the weight of the mouse thymus at low doses the reduction becoming statistically significant after 2 days. To understand the cause for such thymic involution TCDD-evoked changes in various biochemical parameters in this tissue were examined. The most noticeable change was observed in the increased activity of specific protein-tyrosine kinases and protein kinase C and an increased level of p21{sup ras}-associated binding of ({sup 3}H)GTP. The above changes appear to be a selective effect on these special classes of proteins. It has become apparent that the rise in protein-tyrosine kinase activities becomes significant within 24 hr, whereas the rise in protein kinase C does not become significant until 48 hr. In view of similarities between TCDD and thyroid hormones in causing thymic involution, the levels of c-erb-A expression were assessed in the liver by using avian {sup 32}P-labeled v-erb-A probe and RNA transfer blot hybridization technique. The results clearly indicate that TCDD has the property to elevate levels of mRNA bearing homology to v-erb-A. Based on such observations a hypothesis has been proposed that TCDD owes its potency to its ability to stimulate the expression of one of a family of DNAs bearing homology to v-erb-A and that one of the major consequences of such an action is stimulation of various tyrosine kinases.

  14. Aquatic Natural Areas Analysis and Evaluation: Oak Ridge Reservation

    SciTech Connect (OSTI)

    Baranski, Dr. Michael J. [Catawba College

    2011-04-01T23:59:59.000Z

    This report presents an assessment of the natural area value of eight Aquatic Natural Areas (ANAs) and seven Aquatic Reference Areas (ARAs) on the Oak Ridge Reservation (ORR) in Anderson and Roane Counties in east Tennessee. It follows a previous study in 2009 that analyzed and evaluated terrestrial natural areas on the Reservation. The purpose of both studies was to evaluate and rank those specially designated areas on the Reservation that contain sensitive species, special habitats, and natural area value. Natural areas receive special protections through established statutes, regulations, and policies. The ORR contains 33,542 acres (13,574 ha) administered by the Department of Energy. The surface waters of the Reservation range from 1st-order to 5th-order streams, but the majority of the streams recognized as ANAs and ARAs are 1st- and 2nd-order streams. East Fork Poplar Creek is a 4th-order stream and the largest watershed that drains Reservation lands. All the waters of the Reservation eventually reach the Clinch River on the southern and western boundaries of the ORR. All available information was collected, synthesized, and evaluated. Field observations were made to support and supplement the available information. Geographic information system mapping techniques were used to develop several quantitative attributes about the study areas. Narrative descriptions of each ANA and ARA and tables of numerical data were prepared. Criteria for assessment and evaluation were developed, and eight categories of factors were devised to produce a ranking system. The evaluation factors used in the ranking system were: (A) size of area, (B) percentage of watershed protected, (C) taxa present with protected status, (D) overall biotic diversity, (E) stream features, (F) water quality and use support ratings, (G) disturbance regime, and (H) other factors. Each factor was evaluated on a 5-point ranking scale (0-4), and each area received a composite score, where 32 was the maximum score possible. A highly ranked ANA or ARA is one that is large in size compared to other areas, includes a greater proportion of the watershed within Reservation boundaries, contains a number of status taxa at high densities, exhibits a high overall biodiversity, has very good or excellent habitat and water quality, is well protected and isolated from disturbances, and shows several other characteristics that contribute to natural area value. In this report, the term 'natural area' is loosely defined as a terrestrial or aquatic system that exhibits, or is thought to exhibit, high natural integrity and other significant natural values. The purpose of the present study is to evaluate and rank the currently recognized Aquatic Natural Areas (ANAs) and Aquatic Reference Areas (ARAs) on the Oak Ridge Reservation (ORR) for their natural area value. A previous study (Baranski 2009) analyzed, evaluated, and ranked terrestrial areas (Natural Areas [NAs], Reference Areas [RAs], and Cooperative Management Areas [CMAs]) on the ORR for natural area value, and a precise methodology for natural area evaluation was developed. The present study is intended to be a complement and companion to the terrestrial area study and attempts to employ a similar methodology for aquatic areas so that aquatic and terrestrial areas can be compared on a similar scale. This study specifically develops criteria for assessing the ecological, biodiversity, and natural area importance and significance of aquatic systems on the Reservation in a relevant and consistent manner. The information can be integrated into the Tennessee Natural Heritage Program (http://tn.gov/environment/na/nhp.shtml) system and applied to potential new aquatic areas. Further, the information will be useful in planning, management, and protection efforts on the ORR.

  15. Status and progress of the RERTR program in the year 2000.

    SciTech Connect (OSTI)

    Travelli, A.

    2000-09-28T23:59:59.000Z

    This paper describes the progress achieved by the Reduced Enrichment for Research and Test Reactors (RERTR) Program in collaboration with its many international partners during the year 2000 and discusses the main activities planned for the year 2001. The past year was characterized by important accomplishments and events for the RERTR program. Four additional shipments containing 503 spent fuel assemblies from foreign research reactors were accepted by the U.S. Altogether, 3,740 spent fuel assemblies from foreign research reactors have been received by the U.S. under the acceptance policy. Postirradiation examinations of three batches of microplates have continued to reveal excellent irradiation behavior of U-MO dispersion fuels in a variety of compositions and irradiating conditions. h-radiation of two new batches of miniplates of greater sizes is in progress in the ATR to investigate me swelling behavior of these fuels under prototypic conditions. These materials hold the promise of achieving the program goal of developing LEU research reactor fuels with uranium densities in the 8-9 g /cm{sup 3} range. Qualification of the U-MO dispersion fuels is proceeding on schedule. Test fuel elements with 6 gU/cm{sup 3} are being fabricated by BWXT and are scheduled to begin undergoing irradiation in the HFR-Petten in the spring of 2001, with a goal of qualifying this fuel by the end of 2003. U-Mo with 8-9 gU/cm{sup 3} is planned to be qualified by the end of 2005. Joint LEU conversion feasibility studies were completed for HFR-Petten and for SAFARI-1. Significant improvements were made in the design of LEU metal-foil annular targets that would allow efficient production of fission {sup 99}Mo. Irradiations in the RAS-GAS reactor showed that these targets can formed from aluminum tubes, and that the yield and purity of their product from the acidic process were at least as good as those from the HEU Cintichem targets. Progress was made on irradiation testing of LEU UO{sub 2} dispersion fuel and on LEU conversion feasibility studies in the Russian RERTR program. Conversion of the BER-11reactor in Berlin, Germany, was completed and conversion of the La Reins reactor in Santiago, Chile, began. These are exciting times for the program. In the fuel development area, the RERTR program is aggressively pursuing qualification of high-density LEU U-Mo dispersion fuels, with the dual goal of enabling fi.uther conversions and of developing a substitute for LEU silicide fuels that can be more easily disposed of after expiration of the FRR SNF Acceptance Program. The {sup 99}Mo effort has reached the point where it appears feasible for all the {sup 99}Mo producers of the world to agree jointly to a common course of action leading to the elimination of HEU use in their processes. As in the past, the success of the RERTR program will depend on the international friendship and cooperation that has always been its trademark.

  16. Assssment and Mapping of the Riverine Hydrokinetic Resource in the Continental United States

    SciTech Connect (OSTI)

    Jacobson, Paul T. [Electric Power Research Institute; Ravens, Thomas M. [University of Alaska Anchorage; Cunningham, Keith W. [University of Alaska Fairbanks; Scott, George [National Renewable Energy Laboratory

    2012-12-14T23:59:59.000Z

    The U.S. Department of Energy (DOE) funded the Electric Power Research Institute and its collaborative partners, University of Alaska ? Anchorage, University of Alaska ? Fairbanks, and the National Renewable Energy Laboratory, to provide an assessment of the riverine hydrokinetic resource in the continental United States. The assessment benefited from input obtained during two workshops attended by individuals with relevant expertise and from a National Research Council panel commissioned by DOE to provide guidance to this and other concurrent, DOE-funded assessments of water based renewable energy. These sources of expertise provided valuable advice regarding data sources and assessment methodology. The assessment of the hydrokinetic resource in the 48 contiguous states is derived from spatially-explicit data contained in NHDPlus ?a GIS-based database containing river segment-specific information on discharge characteristics and channel slope. 71,398 river segments with mean annual flow greater than 1,000 cubic feet per second (cfs) mean discharge were included in the assessment. Segments with discharge less than 1,000 cfs were dropped from the assessment, as were river segments with hydroelectric dams. The results for the theoretical and technical resource in the 48 contiguous states were found to be relatively insensitive to the cutoff chosen. Raising the cutoff to 1,500 cfs had no effect on estimate of the technically recoverable resource, and the theoretical resource was reduced by 5.3%. The segment-specific theoretical resource was estimated from these data using the standard hydrological engineering equation that relates theoretical hydraulic power (Pth, Watts) to discharge (Q, m3 s-1) and hydraulic head or change in elevation (??, m) over the length of the segment, where ? is the specific weight of water (9800 N m-3): ??? = ? ? ?? For Alaska, which is not encompassed by NPDPlus, hydraulic head and discharge data were manually obtained from Idaho National Laboratory?s Virtual Hydropower Prospector, Google Earth, and U.S. Geological Survey gages. Data were manually obtained for the eleven largest rivers with average flow rates greater than 10,000 cfs and the resulting estimate of the theoretical resource was expanded to include rivers with discharge between 1,000 cfs and 10,000 cfs based upon the contribution of rivers in the latter flow class to the total estimate in the contiguous 48 states. Segment-specific theoretical resource was aggregated by major hydrologic region in the contiguous, lower 48 states and totaled 1,146 TWh/yr. The aggregate estimate of the Alaska theoretical resource is 235 TWh/yr, yielding a total theoretical resource estimate of 1,381 TWh/yr for the continental US. The technically recoverable resource in the contiguous 48 states was estimated by applying a recovery factor to the segment-specific theoretical resource estimates. The recovery factor scales the theoretical resource for a given segment to take into account assumptions such as minimum required water velocity and depth during low flow conditions, maximum device packing density, device efficiency, and flow statistics (e.g., the 5 percentile flow relative to the average flow rate). The recovery factor also takes account of ?back effects? ? feedback effects of turbine presence on hydraulic head and velocity. The recovery factor was determined over a range of flow rates and slopes using the hydraulic model, HEC-RAS. In the hydraulic modeling, presence of turbines was accounted for by adjusting the Manning coefficient. This analysis, which included 32 scenarios, led to an empirical function relating recovery factor to slope and discharge. Sixty-nine percent of NHDPlus segments included in the theoretical resource estimate for the contiguous 48 states had an estimated recovery factor of zero. For Alaska, data on river slope was not readily available; hence, the recovery factor was estimated based on the flow rate alone. Segment-specific estimates of the theoretical resource were multiplied by the corresponding recovery factor to estimate