Sample records for holiday inn express

  1. Annual Review of BPA-Funded Projects in Natural and Artificial Propagation of Salmonids, March 27-29, 1985, Holiday Inn Airport, Portland, Oregon.

    SciTech Connect (OSTI)

    United States. Bonneville Power Administration.

    1985-04-01T23:59:59.000Z

    The Fish and Wildlife Division of Bonneville Power Administration (BPA) hosted a meeting for contractors to present the results of fiscal year 1984 research conducted to implement the Northwest Power Planning Council's Fish and Wildlife Program. The meeting focused on those projects specifically related to natural and artificial propagation of salmonids. The presentations were held at the Holiday Inn Airport in Portland, Oregon, on March 27-29, 1985. This document contains abstracts of the presentations from that meeting. Section 1 contains abstracts on artificial propagation, fish health, and downstream migration, and Section 2 contains abstracts on natural propagation and habitat improvement. The abstracts are indexed by BPA Project Number and by Fish and Wildlife Program Measure. The registered attendees at the meeting are listed alphabetically in Appendix A and by affiliation in Appendix B.

  2. Biosecurity Programme Board Birmingham Holiday Inn

    E-Print Network [OSTI]

    . Private sector concerns re acute oak decline Action (completed): Stewart Snape would circulate details (Timber Packaging and Pallet Confederation. Summary of Action points Agenda Item Action 3. Matters arising re item 6 on restocking after SPHNs. Action: Roger Coppock to liaise with legal colleagues and set

  3. Holiday Foods.

    E-Print Network [OSTI]

    Reasonover, Frances L.; Sweeten, Mary K.

    1981-01-01T23:59:59.000Z

    . ....... . .... . .......... . ... . .. . ..... . .. . .. . . ... . ... . Serving ... . ................... . ... . . . . . .. .. . . ..... . . . .. .. .. . .. . Clean-up .. . ................ . .... . ....... .. . ... . .. . Holiday Food Service Styles ......... . . ... ................ . Spring..., serve cold foods over beds of ice. Keep hot foods in dishes such as a chafing dish or food warmer . Clean-up Put leftover foods back in the refrigerator as soon after the meal as possible. Foods should not be left out for more than 1 or 2 hours...

  4. The Committee convened in the Clark Room, Holiday Inn Capitol,

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, 2013October5,-

  5. NERSC Holiday Schedule

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Saleshttp://www.fnal.gov/directorate/nalcal/nalcal02_07_05_files/nalcal.gif Directorate - Events -Exascale ScienceHoliday Schedule

  6. Holiday Schedule Get ready for the holidays with the Planetarium's

    E-Print Network [OSTI]

    Huang, Haiying

    This holiday season, we're collecting toys for Cook Children's Hospital. Please help us make, such as extra-solar planets orbiting distant stars," said Levent Gurdemir, the planetarium's director

  7. GovCon Holiday Soiree

    Broader source: Energy.gov [DOE]

    The 6th Annual GovCon Holiday Soiree will be held at the Kennedy Center on Monday, December 8, from 5:30pm to 7:30pm. The event is expected to bring together more than 200 representatives of women-...

  8. The Committee convened in the Clark Room of the Holiday Inn

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, 2013October5,- -

  9. The Committee met in the Columbia Room at the Holiday Inn

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, 2013October5,-+

  10. The Committee met in the Clark Room in the Holiday Inn Capitol,

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, COMMITTEE - - -

  11. The Committee met in the Clark Room in the Holiday Inn Capitol,

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, COMMITTEE - -

  12. The Committee met in the Clark Room of the Capitol Holiday Inn,

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, COMMITTEE -

  13. The Committee met in the Clark Room, Holiday Inn Capitol at 550

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, COMMITTEE -

  14. The Committee met in the Columbia Room at the Holiday Inn Capitol,

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTownDells,1Stocks Nov-14Total DeliveredPrincipalNumberAugust7, COMMITTEE

  15. EECBG Success Story: North Pole's Holiday Wish for an Energy...

    Broader source: Energy.gov (indexed) [DOE]

    Pole's Holiday Wish for an Energy Efficient 2012 EECBG Success Story: North Pole's Holiday Wish for an Energy Efficient 2012 December 23, 2011 - 4:20pm Addthis The city of North...

  16. Y-12 employees make holiday merry for local families | National...

    National Nuclear Security Administration (NNSA)

    Photo Gallery Jobs Apply for Our Jobs Our Jobs Working at NNSA Blog Home NNSA Blog Y-12 employees make holiday merry for local families Y-12 employees make holiday merry for...

  17. happy holidays! FROM THE EXECUTIVE VICE PRESIDENT

    E-Print Network [OSTI]

    Qian, Ning

    Construction in Manhattanville 9 Employee News 12 The Back Page Just by the very nature of our business the holiday season, it's important to pause and recognize all of the hard work of our staff that went's foremost coalition of leaders in sustainable building design and construction. · The Northwest Corner

  18. Inside this issue: Green Holiday Tips 2

    E-Print Network [OSTI]

    Kidd, William S. F.

    Inside this issue: Message 1 Green Holiday Tips 2 Real vs. Fake Tree 3 Semester wrap up: Energy a record number of CFL bulbs being distributed in the living residences, had another great energy campaign such as compost bins, bird feeders, energy/water saving appliances, bikes or bus passes make great gifts! Make

  19. Sales Tax Holiday for Energy-Efficient Appliances

    Broader source: Energy.gov [DOE]

    In November 2007, Maryland enacted legislation creating a sales and use tax "holiday" for certain energy-efficient appliances, beginning in 2011. Under the law, qualifying appliances purchased...

  20. Leveraging Holidays and Other Events | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Network Driving Demand Peer Exchange Call Series: Leveraging Holidays and Other Events, Call Slides and Discussion Summary, November 7, 2013. Call Slides and Discussion Summary...

  1. Keep Foodborne Illness from Spoiling Holiday Parties

    E-Print Network [OSTI]

    Nutrition, Food and

    2008-12-17T23:59:59.000Z

    , and lamb steaks, roasts, and chops: 145 degrees F Pork (all cuts): 160 degrees F Ground beef, veal and lamb: 160 degrees F Poultry (whole, ground, or parts): 165 degrees F Egg dishes: 160 degrees F Fish: 145 degrees F After the foods are prepared, make... sure they are served soon or kept at 140 degrees F or higher so bacteria don?t have a chance to grow. If you are serving foods to holiday guests buffet-style, plate up extra serving platters and dishes ahead of time and keep them in a hot oven (set...

  2. Michelle L. Holiday | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33 111 1,613 122Commercial602 1,39732onMake Your Next RoadMichelle L. Holiday About Us Michelle L.

  3. Informations et rservations : ce.pv-holidays.com

    E-Print Network [OSTI]

    Arleo, Angelo

    Informations et réservations : ce.pv-holidays.com * Offre valable pour tout séjour de 7 nuits détails sur ce.pv-holidays.com. Offre valable sur l'hébergement seul (hors frais de dossier, prestations cumulable avec votre remise partenaire, toute offre promotionnelle ou réductions. PV-CPDistribution, Société

  4. Sales Tax Holiday for Energy-Efficient Appliances

    Broader source: Energy.gov [DOE]

    The state of Missouri offers consumers a seven-day ''exemption from state sales taxes'' on certain Energy Star certified new appliances. The state sales tax holiday, known as the "Show-Me Green...

  5. SRR Staff Send the Holidays to Soldiers Overseas

    Broader source: Energy.gov [DOE]

    A request for razors from a U.S. Army private serving in Afghanistan transformed into a full-scale holiday gift rescue operation by employees of the Savannah River Site’s liquid waste contractor,...

  6. Happy Holidays from Clemson December 2006 Dear Friends and Family,

    E-Print Network [OSTI]

    Childress, Michael J.

    Happy Holidays from Clemson December 2006 Dear Friends and Family, Can it be that another year has a wonderful time enjoying the beautiful Lamar Valley. Mom, Dad and Shala also made a trip to Snowbird, UT

  7. Observed Holiday Aerosol Reduction and Temperature Cooling over East Asia

    SciTech Connect (OSTI)

    Gong, Daoyi; Wang, Wenshan; Qian, Yun; Bai, Wenbing; Guo, Yuanxi; Mao, Rui

    2014-06-16T23:59:59.000Z

    The Spring Festival air pollution in China was investigated using the long-term observations from 2001-2012 over 323 stations. During the Spring Festival with nearly half of urban population leaving the cities for holidays, the particulate matter (PM10) concentration is about 24.5?gm-3 (23%) lower than normal days. Associated with the national-wide burning of firework, the PM10 concentration sharply increases to 123.8?gm-3 at Chinese New Year Day (increment of 35%). Similar to PM10, the SO2 and NO2 decrease from high values in normal days to a holiday minimum with reduction of 23.3% and 30.6%, respectively. The NO2 has no peak in New Year Day because of the different emission source. The night mean and minimum temperature co-vary with PM10. Both nighttime mean and minimum temperature decrease by about 2.1°C during the holidays. And in association with the pollution jump at New Year Day the night temperature simultaneously increase by about 0.89°C. The in-phase co-variations between PM10 and night temperature suggest an overall warming effect of holiday aerosol during winter in China.

  8. University Fire Marshal's Office Holiday Fire Safety Information

    E-Print Network [OSTI]

    Straight, Aaron

    festive space heaters... As you un-box those lights, wires and bearers of holiday warmth, it is also heaters. Now is the time--as you set them up--to ensure that you do so providing for the safety of your must be kept in water at all times to slow the natural drying process. Approved Tree Lots ­ Licensed

  9. Sonoma Mission Inn Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎SolarCity Corp Jump to:Solibro ABSonatrachSonoma Mission Inn

  10. Fountain Inn, South Carolina: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump to:ar-80m.pdfFillmoreGabbsSalonga, New York:Rhode Island:Fostoria,Inn,

  11. Add to your Holiday menu: "Canap ", a glass of punch during cocktail hour

    E-Print Network [OSTI]

    Add to your Holiday menu: "Canapé ", a glass of punch during cocktail hour and ½ of bottle of wine per person For only $27.00 per person

  12. Energy Saving Holiday Kitchen Trivia | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33 1112011AT&T,OfficeEnd of Year 2010 SNFEnergy Policy ActEnergy |Division, OAPMSaving Holiday

  13. Leveraging Holidays and Other Events | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreaking ofOil & Gas »of EnergyLearningMarchDepartmentofHolidays and Other

  14. Mythologies of an American everyday landscape : Henry Ford at the Wayside Inn

    E-Print Network [OSTI]

    Wortham, Brooke Danielle

    2006-01-01T23:59:59.000Z

    Ford purchased property in 1923 in Sudbury, Massachusetts in order to preserve an historic inn associated with the poet Henry Wadsworth Longfellow. Over the next twenty years, his mission expanded to create an idealized ...

  15. ARLINGTON/DEFOREST AREA CATERING INFORMATION

    E-Print Network [OSTI]

    Balser, Teri C.

    ARLINGTON/DEFOREST AREA CATERING INFORMATION Rude's Family Catering DeForest, WI 608-846-5959 (Debbie) Roadside Grill DeForest, WI 608-846-1874 (Pete) Piggly Wiggly Poynette, WI 608-635-2647 (Heidi INFORMATION Holiday Inn Express 7184 Morrisonville Rd. DeForest, WI 53532 608-846-8686 toll free 800-HOLIDAY

  16. Memorial Day Weekend Sales Tax Holiday for Energy-Efficient Products

    Broader source: Energy.gov [DOE]

    Purchases of certain energy-efficient products during Memorial Day weekend are exempt from the state sales and use tax.* This amounts to a three-day tax holiday beginning on the Saturday preceding...

  17. Happy Holidays from Boise State Transportation & Parking Services! Some information for the season

    E-Print Network [OSTI]

    Barrash, Warren

    Happy Holidays from Boise State Transportation & Parking Services! Some information for the season, and accessories, visit http://rec.boisestate.edu/clc/, or call 426-RIDE (7433). Participate in the FREE Valley

  18. Fe-doped InN layers grown by molecular beam epitaxy

    SciTech Connect (OSTI)

    Wang Xinqiang; Liu Shitao; Ma Dingyu; Zheng Xiantong; Chen Guang; Xu Fujun; Tang Ning; Shen Bo [State Key Laboratory of Artificial Microstructure and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871 (China); Zhang Peng; Cao Xingzhong; Wang Baoyi [State Key Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049 (China); Huang Sen; Chen, Kevin J. [Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology (Hong Kong); Zhou Shengqiang [Institute of Ion Beam Physics and Materials Research, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), 01314 Dresden (Germany); Yoshikawa, Akihiko [Center for SMART Green Innovation Research, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522 (Japan)

    2012-10-22T23:59:59.000Z

    Iron(Fe)-doped InN (InN:Fe) layers have been grown by molecular beam epitaxy. It is found that Fe-doping leads to drastic increase of residual electron concentration, which is different from the semi-insulating property of Fe-doped GaN. However, this heavy n-type doping cannot be fully explained by doped Fe-concentration ([Fe]). Further analysis shows that more unintentionally doped impurities such as hydrogen and oxygen are incorporated with increasing [Fe] and the surface is degraded with high density pits, which probably are the main reasons for electron generation and mobility reduction. Photoluminescence of InN is gradually quenched by Fe-doping. This work shows that Fe-doping is one of good choices to control electron density in InN.

  19. Spectral dependence of third-order nonlinear optical properties in InN

    SciTech Connect (OSTI)

    Ahn, H., E-mail: hyahn@mail.nctu.edu.tw; Lee, M.-T.; Chang, Y.-M. [Department of Photonics and Institute of Electro-Optical Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan (China)

    2014-05-19T23:59:59.000Z

    We report on the nonlinear optical properties of InN measured in a wide near-infrared spectral range with the femtosecond Z-scan technique. The above-bandgap nonlinear absorption in InN is found to originate from the saturation of absorption by the band-state-filling and its cross-section increases drastically near the bandgap energy. With below-bandgap excitation, the nonlinear absorption undergoes a transition from saturation absorption (SA) to reverse-SA (RSA), attributed to the competition between SA of band-tail states and two-photon-related RSA. The measured large nonlinear refractive index of the order of 10{sup ?10} cm{sup 2}/W indicates InN as a potential material for all-optical switching and related applications.

  20. Growth mechanism and microstructure of low defect density InN (0001) In-face thin films on Si (111) substrates

    SciTech Connect (OSTI)

    Kehagias, Th.; Dimitrakopulos, G. P.; Koukoula, T.; Komninou, Ph. [Department of Physics, Aristotle University of Thessaloniki, 54124 Thessaloniki (Greece)] [Department of Physics, Aristotle University of Thessaloniki, 54124 Thessaloniki (Greece); Ajagunna, A. O.; Georgakilas, A. [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, 71110 Heraklion-Crete (Greece) [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, 71110 Heraklion-Crete (Greece); Physics Department, University of Crete, P.O. Box 2208, 71003 Heraklion-Crete (Greece); Tsagaraki, K. [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, 71110 Heraklion-Crete (Greece)] [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, 71110 Heraklion-Crete (Greece); Adikimenakis, A. [Physics Department, University of Crete, P.O. Box 2208, 71003 Heraklion-Crete (Greece)] [Physics Department, University of Crete, P.O. Box 2208, 71003 Heraklion-Crete (Greece)

    2013-10-28T23:59:59.000Z

    Transmission electron microscopy has been employed to analyze the direct nucleation and growth, by plasma-assisted molecular beam epitaxy, of high quality InN (0001) In-face thin films on (111) Si substrates. Critical steps of the heteroepitaxial growth process are InN nucleation at low substrate temperature under excessively high N-flux conditions and subsequent growth of the main InN epilayer at the optimum conditions, namely, substrate temperature 400–450 °C and In/N flux ratio close to 1. InN nucleation occurs in the form of a very high density of three dimensional (3D) islands, which coalesce very fast into a low surface roughness InN film. The reduced reactivity of Si at low temperature and its fast coverage by InN limit the amount of unintentional Si nitridation by the excessively high nitrogen flux and good bonding/adhesion of the InN film directly on the Si substrate is achieved. The subsequent overgrowth of the main InN epilayer, in a layer-by-layer growth mode that enhances the lateral growth of InN, reduces significantly the crystal mosaicity and the density of threading dislocations is about an order of magnitude less compared to InN films grown using an AlN/GaN intermediate nucleation/buffer layer on Si. The InN films exhibit the In-face polarity and very smooth atomically stepped surfaces.

  1. David J. Innes Sexual reproduction of Daphnia pulex in a temporary habitat

    E-Print Network [OSTI]

    Innes, David J.

    determination á Reproduction Introduction Genotypic sex determination occurs when sex is deter- mined are useful for testing theories on the evolution and maintenance of ESD (Bull 1983; Blackmore and Charnov and high food availability (Hebert Oecologia (1997) 111:53±60 Ó Springer-Verlag 1997 D. J. Innes Department

  2. Probing Nucleation Mechanism of Self-Catalyzed InN Nanostructures

    E-Print Network [OSTI]

    Xu, Gouwei; Li, Zhuangzhi; Baca, Javier; Wu, Judy

    2009-09-16T23:59:59.000Z

    . In the initial stage of the growth, amorphous InNx microparticles of cone shape in liquid phase form with assistance of an InNx wetting layer on the substrate. InN crystallites form inside the cone and serve as the seeds for one-dimensional growth along...

  3. Optical characterization of InN layers grown by high-pressure chemical vapor deposition

    E-Print Network [OSTI]

    Nabben, Reinhard

    and optical properties of InN films grown on sapphire and GaN/sapphire templates. Results obtained from Raman analyzed in this contribution were grown on GaN/sapphire and sapphire 0001 substrates by HPCVD, utilizing to optical absorption edge estimates obtained from optical transmission spectra analysis. The analysis shows

  4. Optical properties of InN grown on templates with controlled surface polarities

    E-Print Network [OSTI]

    Dietz, Nikolaus

    controlled GaN/sapphire substrates leads to unstrained layers with good structural and optical properties. ß is the choice of the substrate. Typical substrate templates for InN are sapphire, ZnO or GaN [13], with GaN samples [16]. We will demonstrate that the use of polarity controlled GaN/sapphire substrates leads

  5. Nanostructural and electronic properties of polytypes in InN nanocolumns

    SciTech Connect (OSTI)

    Kioseoglou, J.; Koukoula, T.; Komninou, Ph.; Kehagias, Th. [Physics Department, Aristotle University of Thessaloniki, GR-54124 Thessaloniki (Greece)] [Physics Department, Aristotle University of Thessaloniki, GR-54124 Thessaloniki (Greece); Georgakilas, A.; Androulidaki, M. [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, GR-71110 Heraklion, Crete and Department of Physics, University of Crete, P.O. Box 2208, GR-71003 Heraklion, Crete (Greece)] [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, GR-71110 Heraklion, Crete and Department of Physics, University of Crete, P.O. Box 2208, GR-71003 Heraklion, Crete (Greece)

    2013-08-21T23:59:59.000Z

    Transmission electron microscopy techniques and density functional theory calculations were employed to investigate the nanostructural and electronic properties of InN polytypes observed in InN nanocolumns, grown on Si(111) by molecular beam epitaxy. Moiré fringes and alternating hexagonal and cubic lattice stacking sequences along the c-axis, observed among the wurtzite layers, implied the presence of different structures embedded in the basic 2H structure of the nanocolumns. Quantitative electron diffraction analysis and high-resolution image simulations verified the coexistence of the wurtzite structure with the 4H, 6H, and the 3C zinc-blende structural polytypes. Total energies calculations established the 2H wurtzite structure as the most stable polytype. The band gap of all polytypes was found direct with the energies and the band gaps of the 4H (E{sub g} = 0.64 eV) and 6H (E{sub g} = 0.60 eV) structures calculated between the corresponding values of the 2H (E{sub g} = 0.75 eV) and 3C (E{sub g} = 0.49 eV) basic structures. Theoretical and experimental analysis showed that at the initial stages of growth InN nanocolumns were under tensile strain along both the basal plane and growth direction. Structural polytypes were then introduced in the form of embedded inclusions to accommodate the excess tensile strain along the growth direction, allowing the entire process of polymorphism to be the dominant strain relaxation mechanism of InN nanocolumns. Moreover, the lattice and energetic properties and band gap values of InN polytypes showed a linear dependence on hexagonality, while the presence of polytypes led to a characteristic broadening of the photoluminescence emission peak toward lower emission energies.

  6. Dendrochronology of the Wixson Road Log Cabin and Benjamin Patterson Inn, Corning-Painted Post Historical Society, Corning, NY

    E-Print Network [OSTI]

    Manning, Sturt

    in 1838, 1855, and again in 1863. In the NYS Census for 1865, the house was described as "log Patterson Inn with its well- documented 1796 building date and the patterns of a modern oak chronology

  7. NANO EXPRESS Epitaxial Catalyst-Free Growth of InN Nanorods on c-Plane

    E-Print Network [OSTI]

    Shalish, Ilan

    -009-9276-z #12;75 mm quartz tube, placed in a three-zone Mellen horizontal furnace. Quartz insert catalyst has been reported [3], but due to its relatively low efficiency, it typically requires large flow with a rectangular cross-section is employed to produce laminar flow in the growth zone and to suppress turbulence

  8. Evaluation of fracture risk and potential drug holidays for postmenopausal women on long-term bisphosphonate therapy

    E-Print Network [OSTI]

    Kostoff, Matthew D.; Saseen, Joseph J.; Borgelt, Laura M.

    2014-04-28T23:59:59.000Z

    holiday. Design: Retrospective electronic health record review. Setting: Eight primary care clinics within a university-based health care system. Patients: A total of 201 postmenopausal women of ages 55–89 years, with osteopenia or osteoporosis, prescribed...

  9. Valence band density of states of zinc-blende and wurtzite InN from x-ray photoemission spectroscopy and first-principles calculations

    E-Print Network [OSTI]

    As, Donat Josef

    Valence band density of states of zinc-blende and wurtzite InN from x-ray photoemission for wurtzite InN 112¯0 are shown to yield a VB-DOS similar to that of zinc-blende InN, although the nonzero the thermodynamically stable phase is the wurtzite 2H polymorph4 wz-InN , judicious choice of substrate material

  10. Effect of reactor pressure on the electrical and structural properties of InN epilayers grown by high-pressure chemical vapor deposition

    E-Print Network [OSTI]

    Nabben, Reinhard

    Effect of reactor pressure on the electrical and structural properties of InN epilayers grown://avspublications.org/jvsta/about/rights_and_permissions #12;Effect of reactor pressure on the electrical and structural properties of InN epilayers grown-atmospheric reactor pressures (2.5­18.5 bar) on the electrical and structural properties of InN epilayers deposited

  11. CRSPflyer15

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    May 21, 2015 11 am - 3 pm Holiday Inn & Suites - SLC Airport West 5001 Wiley Post Way Salt Lake City, UT 84116 801-741-1800 The Holiday Inn will provide a free airport shuttle...

  12. Fundamental optical properties of InN grown by epitaxial lateral overgrowth method

    SciTech Connect (OSTI)

    Kametani, Tatsuma; Kamimura, Jumpei; Inose, Yuta; Kunugita, Hideyuki; Kikuchi, Akihiko; Kishino, Katsumi; Ema, Kazuhiro [Department of Engineering and Applied Science, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan)

    2013-12-04T23:59:59.000Z

    Optical properties of InN grown by the epitaxial lateral overgrowth (ELO) method have been studied using photoluminescence (PL) and excitation-correlation (EC) measurements. The PL spectrum is analyzed by free-electron recombination band (FERB) model, which shows that the ELO sample has a very low background carrier concentration (n=5.5*10{sup 16}[cm{sup ?3]}). EC measurements show that the dependences of the band gap renormalization and Auger effect on the carrier concentrations are similar in spite of the different physical origins.

  13. Metallicity of InN and GaN surfaces exposed to NH{sub 3}.

    SciTech Connect (OSTI)

    Walkosz, W.; Zapol, P.; Stephenson, G. B. (Materials Science Division)

    2012-01-01T23:59:59.000Z

    A systematic study of energies and structures of InN and GaN (0001) surfaces exposed to NH{sub 3} and its decomposition products was performed with first-principles methods. A phenomenological model including electron counting contributions is developed based on calculated DFT energies and is used to identify low-energy structures. These predictions are checked with additional DFT calculations. The equilibrium phase diagrams are found to contain structures that violate the electron counting rule. Densities of states for these structures indicate n-type conductivity, consistent with available experimental results.

  14. Effect of dislocation scattering on the transport properties of InN grown on GaN substrates by molecular beam epitaxy

    E-Print Network [OSTI]

    Effect of dislocation scattering on the transport properties of InN grown on GaN substrates on GaN substrates by plasma-assisted molecular beam epitaxy. They have found a strong correlation, optical, and transport properties of InN on GaN substrates. In this work, we have studied the MBE growth

  15. Your Holidays ... Brought to You by Fuel Cells | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you sure you wantJoin us for|IdahotheWhat is the FOIA ? WhatWorkHolidays ...

  16. Universality of electron accumulation at wurtzite c-and a-plane and zinc-blende InN surfaces

    E-Print Network [OSTI]

    As, Donat Josef

    Universality of electron accumulation at wurtzite c- and a-plane and zinc- blende InN surfaces P. D 27 August 2007 Electron accumulation is found to occur at the surface of wurtzite 112¯0 , 0001.6 Experimental studies to date have focused on wurtzite c-plane surfaces,1­3,7 although pre- vious

  17. Lasers for Hyperfine State Detection and Cooling of Ytterbium Ions Sarah Innes-Gold, University of Washington REU

    E-Print Network [OSTI]

    Washington at Seattle, University of - Department of Physics, Electroweak Interaction Research Group

    . Researchers in various groups are investigating many possible systems in- cluding photons, superconductingLasers for Hyperfine State Detection and Cooling of Ytterbium Ions Sarah Innes-Gold, University of Washington REU 29 August 2014 Abstract The purpose of this REU project was to implement a system of lasers

  18. Effect of Mg doping on the structural and free-charge carrier properties of InN films

    SciTech Connect (OSTI)

    Xie, M.-Y.; Ben Sedrine, N.; Hung, L.; Monemar, B.; Darakchieva, V., E-mail: vanya@ifm.liu.se [Department of Physics, Chemistry, and Biology, IFM, Linköping University, SE-581 83 Linköping (Sweden); Schöche, S.; Hofmann, T.; Schubert, M. [Center for Nanohybrid Functional Materials, Department of Electrical Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0511 (United States); Wang, X. [State Key Laboratory of Artificial Microstructure and Mesoscopic Physics, Peking University, Beijing (China); Yoshikawa, A. [Center for SMART Green Innovation Research, Chiba University, Chiba (Japan); Department of Information and Communication Engineering, Kogakuin University, Tokyo (Japan); Wang, K.; Araki, T. [Department of Photonics, Ritsumeikan University, 1-1-1 Noji Higashi, Kusatsu, Shiga 525-8577 (Japan); Nanishi, Y. [Department of Photonics, Ritsumeikan University, 1-1-1 Noji Higashi, Kusatsu, Shiga 525-8577 (Japan); WCU Hybrid Materials Program, Department of Materials Science and Engineering, Seoul National University, Seoul 151-744 (Korea, Republic of)

    2014-04-28T23:59:59.000Z

    We present a comprehensive study of free-charge carrier and structural properties of two sets of InN films grown by molecular beam epitaxy and systematically doped with Mg from 1.0?×?10{sup 18}?cm{sup ?3} to 3.9?×?10{sup 21}?cm{sup ?3}. The free electron and hole concentration, mobility, and plasmon broadening parameters are determined by infrared spectroscopic ellipsometry. The lattice parameters, microstructure, and surface morphology are determined by high-resolution X-ray diffraction and atomic force microscopy. Consistent results on the free-charge carrier type are found in the two sets of InN films and it is inferred that p-type conductivity could be achieved for 1.0?×?10{sup 18}?cm{sup ?3}???[Mg]???9.0?×?10{sup 19}?cm{sup ?3}. The systematic change of free-charge carrier properties with Mg concentration is discussed in relation to the evolution of extended defect density and growth mode. A comparison between the structural characteristics and free electron concentrations in the films provides insights in the role of extended and point defects for the n-type conductivity in InN. It further allows to suggest pathways for achieving compensated InN material with relatively high electron mobility and low defect densities. The critical values of Mg concentration for which polarity inversion and formation of zinc-blende InN occurred are determined. Finally, the effect of Mg doping on the lattice parameters is established and different contributions to the strain in the films are discussed.

  19. Solar hot water system installed at Quality Inn, Key West, Florida. Final report

    SciTech Connect (OSTI)

    Not Available

    1980-04-01T23:59:59.000Z

    The solar energy hot water system installed in the Quality Inn, Key West, Florida, which consists of four buildings, is described. Three buildings are low-rise, two-story buildings containing 100 rooms. The fourth is a four-story building with 48 rooms. The solar system was designed to provide approximately 50% of the energy required for the domestic hot water system. The solar system consists of approximately 1400 ft/sup 2/ of flat plate collector, two 500 gal storage tanks, a circulating pump, and a controller. Operation of the system was begun in April 1978, and has continued to date with only three minor interruptions for pump repair. In the first year of operation, it was determined that the use of the solar facility resulted in 40% fuel savings.

  20. 2012 BATTERIES GORDON RESEARCH CONFERENCE, MARCH 4-9, 2012

    SciTech Connect (OSTI)

    Stephen Harris

    2012-03-09T23:59:59.000Z

    The Gordon Research Conference on BATTERIES was held at Four Points Sheraton / Holiday Inn Express, Ventura, California, March 4-9, 2012. The Conference was well-attended with 176 participants. Gordon Research Conferences does not permit publication of meeting proceedings.

  1. Dislocation reduction via selective-area growth of InN accompanied by lateral growth by rf-plasma-assisted molecular-beam epitaxy

    SciTech Connect (OSTI)

    Kamimura, Jumpei [Department of Engineering and Applied Sciences, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan); CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 330-0012 (Japan); Kishino, Katsumi; Kikuchi, Akihiko [Department of Engineering and Applied Sciences, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan); CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 330-0012 (Japan); Sophia Nanotechnology Research Center, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554 (Japan)

    2010-10-04T23:59:59.000Z

    We investigated the selective-area growth (SAG) of InN by rf-plasma-assisted molecular-beam epitaxy using molybdenum (Mo)-mask-patterned sapphire (0001) substrates, which resulted in the formation of regularly arranged N-polar InN microcrystals. Transmission electron microscopy observation confirmed that the laterally grown side areas were nearly dislocation-free, although many threading dislocations (10{sup 9}-10{sup 10} cm{sup -2}) were generated at the InN/sapphire interface and propagated into the center of the InN microcrystals along the crystal c-axis. The laterally grown InN microcrystals exhibited narrow near-IR emission spectra with a peak photon energy of 0.627 eV and a linewidth of 39 meV at room temperature.

  2. Band gap and band parameters of InN and GaN from quasiparticle energy calculations based on exact-exchange density-functional theory

    E-Print Network [OSTI]

    Band gap and band parameters of InN and GaN from quasiparticle energy calculations based on exact; published online 20 October 2006 The authors have studied the electronic structure of InN and GaN employing. © 2006 American Institute of Physics. DOI: 10.1063/1.2364469 The group III-nitrides AlN, GaN, and In

  3. A4.2 Raman and IR studies of InN C. Wetzel and I. Akasaki

    E-Print Network [OSTI]

    Wetzel, Christian M.

    and reflection in the near infrared spectral region have been employed to study the fundamental bandgap, phononsN was an extrapolation of the Ga1-xInxN (0 x 1) alloy modes in reflection towards the binary compound [1]. A typically) in the infrared and must also be accounted for the broadened Reststrahlen band in pure InN films (e.g. in [1

  4. Hole transport and photoluminescence in Mg-doped InN

    SciTech Connect (OSTI)

    Miller, N.; Ager III, J. W.; Smith III, H. M.; Mayer, M. A.; Yu, K. M.; Haller, E. E.; Walukiewicz, W.; Schaff, W. J.; Gallinat, C.; Koblmuller, G.; Speck, J. S.

    2010-03-24T23:59:59.000Z

    Hole conductivity and photoluminescence were studied in Mg-doped InN films grown by molecular beam epitaxy. Because surface electron accumulation interferes with carrier type determination by electrical measurements, the nature of the majority carriers in the bulk of the films was determined using thermopower measurements. Mg concentrations in a"window" from ca. 3 x 1017 to 1 x 1019 cm-3 produce hole-conducting, p-type films as evidenced by a positive Seebeck coecient. This conclusion is supported by electrolyte-based capacitance voltage measurements and by changes in the overall mobility observed by Hall effect, both of which are consistent with a change from surface accumulation on an n-type film to surface inversion on a p-type film. The observed Seebeck coefficients are understood in terms of a parallel conduction model with contributions from surface and bulk regions. In partially compensated films with Mg concentrations below the window region, two peaks are observed in photoluminescence at 672 meV and at 603 meV. They are attributed to band-to-band and band-to-acceptor transitions, respectively, and an acceptor binding energy of ~;;70 meV is deduced. In hole-conducting films with Mg concentrations in the window region, no photoluminescence is observed; this is attributed to electron trapping by deep states which are empty for Fermi levels close to the valence band edge.

  5. Optical characterization of free electron concentration in heteroepitaxial InN layers using Fourier transform infrared spectroscopy and a 2 Multiplication-Sign 2 transfer-matrix algebra

    SciTech Connect (OSTI)

    Katsidis, C. C. [Department of Materials Science and Technology, University of Crete, P.O. Box 2208, 71003 Heraklion-Crete (Greece); Ajagunna, A. O.; Georgakilas, A. [Microelectronics Research Group, IESL, FORTH, P.O. Box 1385, 71110 Heraklion-Crete (Greece); Physics Department, University of Crete, P.O. Box 2208, 71003 Heraklion-Crete (Greece)

    2013-02-21T23:59:59.000Z

    Fourier Transform Infrared (FTIR) reflectance spectroscopy has been implemented as a non-destructive, non-invasive, tool for the optical characterization of a set of c-plane InN single heteroepitaxial layers spanning a wide range of thicknesses (30-2000 nm). The c-plane (0001) InN epilayers were grown by plasma-assisted molecular beam epitaxy (PAMBE) on GaN(0001) buffer layers which had been grown on Al{sub 2}O{sub 3}(0001) substrates. It is shown that for arbitrary multilayers with homogeneous anisotropic layers having their principal axes coincident with the laboratory coordinates, a 2 Multiplication-Sign 2 matrix algebra based on a general transfer-matrix method (GTMM) is adequate to interpret their optical response. Analysis of optical reflectance in the far and mid infrared spectral range has been found capable to discriminate between the bulk, the surface and interface contributions of free carriers in the InN epilayers revealing the existence of electron accumulation layers with carrier concentrations in mid 10{sup 19} cm{sup -3} at both the InN surface and the InN/GaN interface. The spectra could be fitted with a three-layer model, determining the different electron concentration and mobility values of the bulk and of the surface and the interface electron accumulation layers in the InN films. The variation of these values with increasing InN thickness could be also sensitively detected by the optical measurements. The comparison between the optically determined drift mobility and the Hall mobility of the thickest sample reveals a value of r{sub H} = 1.49 for the Hall factor of InN at a carrier concentration of 1.11 Multiplication-Sign 10{sup 19} cm{sup -3} at 300 Degree-Sign {Kappa}.

  6. The Mercer Clinic Holiday Pet Basket program was started in 1995 by Veterinary Medical Teaching Hospital staff members of the "VOICE" staff newsletter. It has grown due to need over the years from only 25 baskets to 130

    E-Print Network [OSTI]

    Schladow, S. Geoffrey

    The Mercer Clinic Holiday Pet Basket program was started in 1995 by Veterinary Medical Teaching that every year we rely on the kindness of others to help these very special pets who are more than a pet this wonderful tradition alive by contributing any time of year to the Mercer Veterinary Clinic Holiday Pet

  7. Molecular beam epitaxy and structural anisotropy of m-plane InN grown on free-standing GaN

    SciTech Connect (OSTI)

    Koblmueller, G.; Hirai, A.; Wu, F.; Gallinat, C. S.; Speck, J. S. [Materials Department, University of California, Santa Barbara, California 93106-5050 (United States); Metcalfe, G. D.; Shen, H.; Wraback, M. [U. S. Army Research Laboratory, Sensors and Electron Devices Directorate, 2800 Powder Mill Road, Adelphi, Maryland 20783 (United States)

    2008-10-27T23:59:59.000Z

    This study reports on the growth of high-quality nonpolar m-plane [1100] InN films on free-standing m-plane GaN substrates by plasma-assisted molecular beam epitaxy. Optimized growth conditions (In/N ratio {approx}1 and T=390-430 deg. C) yielded very smooth InN films with undulated features elongated along the [1120] orientation. This directionality is associated with the underlying defect structure shown by the anisotropy of x-ray rocking curve widths parallel to the [1120] (i.e., 0.24 deg. - 0.34 deg.) and [0001] (i.e., 1.2 deg. - 2.7 deg.) orientations. Williamson-Hall analysis and transmission electron microscopy identified the mosaic tilt and lateral coherence length and their associations with different densities of dislocations and basal-plane stacking faults. Ultimately, very low band gap energies of {approx}0.67 eV were measured by optical absorption similar to the best c-plane InN.

  8. The Hebrew University Center for Nanoscience and Nanotechnology

    E-Print Network [OSTI]

    Simon, Emmanuel

    The Hebrew University Center for Nanoscience and Nanotechnology Annual Conference 2015 Holiday Inn your work during the Nanoscience and Nanotechnology Center Yearly Conference, which will be held

  9. InGaN/GaN multiple-quantum-well light-emitting diodes with a grading InN composition suppressing the Auger recombination

    E-Print Network [OSTI]

    Demir, Hilmi Volkan

    InGaN/GaN multiple-quantum-well light-emitting diodes with a grading InN composition suppressing://scitation.aip.org/content/aip/journal/apl/105/3?ver=pdfcov Published by the AIP Publishing Articles you may be interested in High efficiency InGaN/GaN (2014); 10.1063/1.4867023 Effect of V-defects on the performance deterioration of InGaN/GaN multiple

  10. Holiday Food Drive

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) EnvironmentalGyroSolé(tm) HarmonicbetandEnergy Energy Department'sHobartFood

  11. Holiday Gift Drive

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) EnvironmentalGyroSolé(tm) HarmonicbetandEnergy Energy Department'sHobartFoodGift

  12. Berkeley Lab Holiday Schedule

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office511041clothAdvanced Materials Advanced Materials Find More Like3.3BenefitsSearch This page has

  13. Point defects introduced by InN alloying into In{sub x}Ga{sub 1-x}N probed using a monoenergetic positron beam

    SciTech Connect (OSTI)

    Uedono, A. [Division of Applied Physics, Faculty of Pure and Applied Science, University of Tsukuba, Tsukuba, Ibaraki 305-8573 (Japan); Tsutsui, T.; Watanabe, T.; Kimura, S.; Zhang, Y. [Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8573 (Japan); Lozac'h, M. [Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8573 (Japan); National Institute for Materials Science, Tsukuba, Ibaraki 305-0044 (Japan); Sang, L. W.; Sumiya, M. [National Institute for Materials Science, Tsukuba, Ibaraki 305-0044 (Japan); Ishibashi, S. [Nanosystem Research Institute (NRI) 'RICS,' National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8568 (Japan)

    2013-03-28T23:59:59.000Z

    Native defects in In{sub x}Ga{sub 1-x}N (x = 0.06-0.14) grown by metal organic chemical vapor deposition were studied using a monoenergetic positron beam. Measurements of Doppler broadening spectra of the annihilation radiation as a function of incident positron energy for In{sub x}Ga{sub 1-x}N showed that vacancy-type defects were introduced with increasing InN composition, and the major defect species was identified as complexes between a cation vacancy and a nitrogen vacancy. The concentration of the divacancy, however, was found to be suppressed by Mg doping. The momentum distribution of electrons at the In{sub x}Ga{sub 1-x}N/GaN interface was close to that in defect-free GaN or In{sub x}Ga{sub 1-x}N, which was attributed to localization of positrons at the interface due to the built-in electric field, and to suppression of positron trapping by vacancy-type defects. We have also shown that the diffusion property of positrons is sensitive to an electric field near the In{sub x}Ga{sub 1-x}N/GaN interface.

  14. InGaN/GaN multiple-quantum-well light-emitting diodes with a grading InN composition suppressing the Auger recombination

    SciTech Connect (OSTI)

    Zhang, Zi-Hui; Liu, Wei; Ju, Zhengang; Tan, Swee Tiam; Ji, Yun; Kyaw, Zabu; Zhang, Xueliang; Wang, Liancheng; Sun, Xiao Wei, E-mail: EXWSUN@ntu.edu.sg, E-mail: VOLKAN@stanfordalumni.org [LUMINOUS Centre of Excellence for Semiconductor Lighting and Displays, School of Electrical and Electronic Engineering, School of Physical and Mathematical Sciences, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore); Demir, Hilmi Volkan, E-mail: EXWSUN@ntu.edu.sg, E-mail: VOLKAN@stanfordalumni.org [LUMINOUS Centre of Excellence for Semiconductor Lighting and Displays, School of Electrical and Electronic Engineering, School of Physical and Mathematical Sciences, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore); Department of Electrical and Electronics, Department of Physics, and UNAM-Institute of Material Science and Nanotechnology, Bilkent University, TR-06800 Ankara (Turkey)

    2014-07-21T23:59:59.000Z

    In conventional InGaN/GaN light-emitting diodes (LEDs), thin InGaN quantum wells are usually adopted to mitigate the quantum confined Stark effect (QCSE), caused due to strong polarization induced electric field, through spatially confining electrons and holes in small recombination volumes. However, this inevitably increases the carrier density in quantum wells, which in turn aggravates the Auger recombination, since the Auger recombination scales with the third power of the carrier density. As a result, the efficiency droop of the Auger recombination severely limits the LED performance. Here, we proposed and showed wide InGaN quantum wells with the InN composition linearly grading along the growth orientation in LED structures suppressing the Auger recombination and the QCSE simultaneously. Theoretically, the physical mechanisms behind the Auger recombination suppression are also revealed. The proposed LED structure has experimentally demonstrated significant improvement in optical output power and efficiency droop, proving to be an effective solution to this important problem of Auger recombination.

  15. Research Brief The Commonwealth's Official Source for Population and Economic Statistics

    E-Print Network [OSTI]

    Development and Much More PENN STATE HARRISBURG ­ Data users from across the Commonwealth are set to gather at the Holiday Inn Harrisburg/Hershey for the Pennsylvania State Data Center's 2012 Annual Data User Conference

  16. Chemical Reactions at Surfaces. Final Progress Report

    SciTech Connect (OSTI)

    None

    2003-02-21T23:59:59.000Z

    The Gordon Research Conference (GRC) on Chemical Reactions at Surfaces was held at Holiday Inn, Ventura, California, 2/16-21/03. Emphasis was placed on current unpublished research and discussion of the future target areas in this field.

  17. Folder Hotels Inhoudsopgave pagina

    E-Print Network [OSTI]

    Franssen, Michael

    + 3 Holiday Inn Eindhoven 4 + 5 Hampshire Hotel-Crown Eindhoven 6 + 7 Best Western Premier Art Hotel Eindhoven 8 + 9 Boutique Hotel Lumière 10 + 11 Sandton Hotel Eindhoven City Centre 12 + 13 Hampshire Hotel Parkzicht Eindhoven 14 + 15 Queen Hotel Eindhoven 16 + 17 Crown Inn Hotel Eindhoven 18 + 19 Hotel La Reine

  18. Post-Holiday Holiday Shopping | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China 2015of 2005UNS Electric, Inc.Department ofAmountAtomic7951Fossil fuel fired

  19. Holiday Plants with Toxic Misconceptions

    E-Print Network [OSTI]

    Evens, Zabrina N; Stellpflug, Samuel J

    2012-01-01T23:59:59.000Z

    1999;41:394-7. the American Association of Poison ControlCenters’ National Poison 21. Kramer R. BittersweetMinnesota Hennepin Regional Poison Center, Minneapolis,

  20. Holiday Plants with Toxic Misconceptions

    E-Print Network [OSTI]

    Evens, Zabrina N; Stellpflug, Samuel J

    2012-01-01T23:59:59.000Z

    the induction of emesis, decontamination, and the use oft coincide with current decontamination recommendations. 16by gastrointestinal decontamination, as 96.2% of treated

  1. Helping make the holidays happier

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cn SunnybankD.jpgHanfordDepartment ofHeat TransferStartupHe!LosHelping

  2. LYAKllOVSKY. v .. ILeIlEv. A.. anJ AGNON. A. jI,tmklling "f JUl1lug~ and in.'tabilili~s ill rock mass by In~"ns of a nun-lin,Jj'I<'s-RaSiM5. South African tnsuunc of Mining and Mctalturgv. 200 I.

    E-Print Network [OSTI]

    Lyakhovsky, Vladimir

    rock mass by In~"ns of a nun-linmrS/.\\' ",,,1 S"ismi,:iry in t;p , LYAKllOVSKY. v .. ILeIlEv. A.. anJ AGNON. A. jI,·tmklling "f JUl1lug~ and in.'tabilili~s ill' *(jeological Surrey of Israel, l erusatem Israel f/SS lntemauonat Limited, l,VelkOIl1, South A/rim i

  3. Experiments in expression recognition

    E-Print Network [OSTI]

    Skelley, James P. (James Paul)

    2005-01-01T23:59:59.000Z

    Despite the significant effort devoted to methods for expression recognition, suitable training and test databases designed explicitly for expression research have been largely neglected. Additionally, possible techniques ...

  4. High expression Zymomonas promoters

    DOE Patents [OSTI]

    Viitanen, Paul V. (West Chester, PA); Tao, Luan (Havertown, PA); Zhang, Yuying (New Hope, PA); Caimi, Perry G. (Kennett Square, PA); McCole, Laura (East Fallowfield, PA): Zhang, Min (Lakewood, CO); Chou, Yat-Chen (Lakewood, CO); McCutchen, Carol M. (Wilmington, DE); Franden, Mary Ann (Centennial, CO)

    2011-08-02T23:59:59.000Z

    Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.

  5. Dr. David Innes Friday Oct. 30 discussion

    E-Print Network [OSTI]

    Innes, David J.

    in rates of evolution Relative rate test (d = branch lengths) dxy = dox + doy A dxz = dox + doz ------------------------------------ dox ­ doy = dxz ­ dyz with molecular clock: dox ­ doy = 0 therefore expect: dxz ­ dyz = 0 dxz = dyz X

  6. High Electron Mobility InN

    E-Print Network [OSTI]

    2007-01-01T23:59:59.000Z

    The AlN/GaN buffer layer and sapphire substrate are alsoc- sapphire substrates with an AlN and/or GaN buffer layer,

  7. Today LED Holiday Lights, Tomorrow the World?

    SciTech Connect (OSTI)

    Gordon, Kelly L.

    2004-12-20T23:59:59.000Z

    This article for The APEM Advantage, the quarterly newsletter of the Association of Professional Energy Managers (APEM) describes the recent increase in the popularity of light emitting diode (LED) lighting and compares LED light output with that of incandescent and compact fluorescent lighting.

  8. Happy Holidays! Oldest and Large~t'

    E-Print Network [OSTI]

    -bearing~tudent group accounts after Dean for Student Life Larry G. Benedict pledged to fund interest 'that would have." . Tracy F. Purinton, assistant dean for student activities, said that there are approximately eight.01) students Farhan I. Merali '05 and Corey A. Kemper G take notes on electronic tablets. Originally intended

  9. Code of Conduct Regarding Holiday Gifts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625govInstrumentstdmadapInactiveVisiting the TWPSuccessAlamosCharacterization2Climate,Cobalt discoveryCode of Conduct

  10. A Factsheet on Holiday Fire Prevention

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch >InternshipDepartment ofAugustDecember8threbuildA Comprehensiveach year

  11. MISC&LLANI:OUS PAPER Aa76-1 PROCEEDINGS, RESEARCH PLANNING

    E-Print Network [OSTI]

    US Army Corps of Engineers

    South American Field Studies of Prospective Biocontrol Agents of Weeds Aquatic Weed Problems in Mexico Program was held at the Holiday Inn Downtown, Charleston, South Carolina, on 22-24 October 1975 Degradation of Silvex by Synergistic Action of Aquatic Microorganisms Integrated Control of Waterhyacinths

  12. This Information can also be found at neon.mems.cmu.edu/rollett/CMSN.Wkshp5.Agenda.pdf

    E-Print Network [OSTI]

    Rollett, Anthony D.

    ), Princeton: Temperature Dependence of Grain Boundary Mobility in 3-D. 2:10 break 2:30 Mikhail Mendelev Jackson: title TBA. 9:20 Daniel Lewis, NIST: 3D Effects in Phase Field Models of Eutectic Growth. 9 set aside at the Gaithersburg Holiday Inn, which will run a shuttle in the morning and evening

  13. 2 Global Gas Turbine News August 2008 There is an old saying that the only constant in life is change. Our

    E-Print Network [OSTI]

    Daraio, Chiara

    infrastructure to address the two-sided effects of imbalances in the supply and demand of fossil fuels, as well, are in high demand. As the largest gathering of research, design and development turbomachinery engineers Holiday Inn Hotel, Milton K eynes Buckinghamshire , UK Presented by the Institution of Diesel and Gas

  14. Character Divination and Kinetic Sculpture in the Central Italian Passeggiata (Ritual Promenade): Interpretive Frameworks and Expressive Practices from a Body-Centered Perspective

    E-Print Network [OSTI]

    Del Negro, Giovanna P.; Berger, Harris M.

    own small plots of land in the surrounding countryside, and farming is a common weekend or holiday activity that contributes to the family larder. In popular memory, Sasso is often viewed as a cosmopolitan village with close affini- ties...

  15. Collective systems for creative expression

    E-Print Network [OSTI]

    Ar?kan, Harun Burak

    2006-01-01T23:59:59.000Z

    This thesis defines collective systems as a unique category of creative expression through the procedures of micro and macro cycles that address the transition from connectivity to collectivity. This thesis discusses the ...

  16. Transmission Electron Microscopy Study of InN Nanorods

    E-Print Network [OSTI]

    Liliental-Weber, Z.; Li, X.; Kryliouk, Olga; Park, H.J.; Mangum, J.; Anderson, T.

    2008-01-01T23:59:59.000Z

    crystals grown on Si and GaN substrate is different. Theon c-Al 2 O 3, GaN , and Si substrates by non-catalytic,

  17. I-N-N Electric Coop, Inc | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup | OpenHunan Runhua New Energy DevelopmentListI Sol Ventures GmbH

  18. sed and awk Regular Expressions

    E-Print Network [OSTI]

    Mohri, Mehryar

    at a time, like grep · Change lines of the file · Non-interactive text editor ­ Editing commands come in order to each input line. · If a command changes the input, subsequent command address will be applied and an action, where the address can be a regular expression or line number. address action command address

  19. Keep Foodborne Illness from Spoiling Holiday Parties (Spanish) 

    E-Print Network [OSTI]

    Nutrition, Food and

    2008-12-17T23:59:59.000Z

    . November 2006. http://www.cfsan. fda.gov/~dms/fsholida.html Producido por AgriLife Communications, El Sistema Texas A&M Las publicaciones de Texas AgriLife Extension se pueden encontrar en Internet en: http://AgriLifebookstore.org Los programas educativos...

  20. Keep Foodborne Illness from Spoiling Holiday Parties (Spanish)

    E-Print Network [OSTI]

    Nutrition, Food and

    2008-12-17T23:59:59.000Z

    . November 2006. http://www.cfsan. fda.gov/~dms/fsholida.html Producido por AgriLife Communications, El Sistema Texas A&M Las publicaciones de Texas AgriLife Extension se pueden encontrar en Internet en: http://AgriLifebookstore.org Los programas educativos...

  1. Sales Tax Holiday for Energy-Efficient Appliances

    Broader source: Energy.gov [DOE]

    '''Note: Legislation enacted in June 2011 ([http://www.ncleg.net/enactedlegislation/sessionlaws/html/2011-2012/sl201... S.B. 267]) that removed geothermal heat pumps from the list of energy systems...

  2. BioSafety On Campus Holiday Fire Safety

    E-Print Network [OSTI]

    Becker, Luann

    to biohazardous or potentially infectious agents. At UCSB, the Biological Safety Program is one of five units an average of 24 civilian deaths, 27 civilian injuries, and $13.3 million in direct property damage per year of the total number of natural Christmas trees sold, which is estimated at 30 million trees, in the United

  3. 2013 UCSC Second Harvest Holiday Food Drive Raffle Raffle Guidelines

    E-Print Network [OSTI]

    California at Santa Cruz, University of

    One bottle Odonata Winery River Skimmer One Annie Glass glass plate - value $40 Two Zip car - Chancellor One bottle Saintsbury Vineyards Carneros Chardonnay One bottle Saintsbury Vineyards Carneros Pinot

  4. SEP Success Story: Solar Field Powers Historic Garden Holiday...

    Energy Savers [EERE]

    agencies and the University of Tennessee at the official opening of the West Tennessee Solar Farm. | Energy Department photo. SEP Success Story: Harvesting the Sun at the West...

  5. EM Waste Isolation Pilot Plant Team's Holiday Spirit Shines | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:RevisedAdvisory Board Contributions EMEM Recovery Act PressEMTacklesTapsof

  6. Energy Saving Holiday Kitchen Trivia | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently20,000 Russian NuclearandJunetrackEllen|July 14, 2014 Outdoor solar lights

  7. Holiday Gifts for Energy Efficiency | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently20,000 RussianBy: Thomas P. D'Agostino,GlenLearning

  8. How Do Holiday Lights Work? | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently20,000 RussianBy: Thomas P. D'Agostino,GlenLearningDepartmentDistributed wind

  9. Buying an Appliance this Holiday Season? ENERGY STAR Products...

    Office of Environmental Management (EM)

    Freezers Room air conditioners Televisions Clothes washers Dishwashers Battery chargers Water heaters Fluorescent lamp ballasts Incandescent reflector lamps If your appliance has...

  10. Energy-Efficient Holiday Decorating Tips | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33 1112011AT&T,OfficeEnd of Year 2010 SNFEnergySession 3 |DepartmentDepartment

  11. Fuel Cell Powers Up Festivities at Secretary Chu's Holiday Party |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't YourTransport inEnergy0.pdfTechnologies Program (FCTP) (Fact

  12. LED Holiday Lights: Festive, Safe, and Efficient! | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't YourTransport(FactDepartment ofLetter Report:40PM to 2:05PMDOE-STD-1107-97LSEED:LDVFrequentlyLED

  13. Leveraging Holidays and Other Events | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't YourTransport(FactDepartment ofLetter Report:40PMDepartment ofs oLetter fromAccidentLeveraging

  14. Workers Across the Nuclear Security Enterprise Give Back This Holiday

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA groupTuba City,EnrichedSupplemental DirectivesWork Schedules | National

  15. SEP Success Story: Solar Field Powers Historic Garden Holiday Display |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn'tOrigin ofEnergy atLLC - FE DKT. 10-160-LNG - ORDER 2913 |Department ofProgramDepartment of

  16. Solar Field Powers Historic Garden Holiday Display | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO Overview OCHCO OverviewRepositoryManagement |Solar Energy Development in

  17. Save Energy on Appliances this Holiday Season | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del Sol HomeFacebook Twitter Principal InvestigatorsSave Energy on Appliances this

  18. July 4th Holiday Update - June 30, 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your Home as Ready for(SC)JointJournal Cover19 July

  19. Tips to Save Energy During the Holidays | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof Energy 2, 2015 - JanuaryTank 48HPublicforManagement andEnergyTimTimothyTips

  20. Holiday City South, New Jersey: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtel Jump to: navigation,Jersey: EnergySpain)HockleyHokeHolderness,

  1. Holiday City-Berkeley, New Jersey: Energy Resources | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtel Jump to: navigation,Jersey:

  2. Holiday Heights, New Jersey: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtel Jump to: navigation,Jersey:Heights, New Jersey: Energy Resources Jump

  3. Tips to Save Energy During the Holidays | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently AskedEnergyIssuesEnergy SolarRadioactive LiquidSavingsAugustPhase 2ATFTips forTips

  4. Save Money with LED Holiday Light Strings | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn April 23, 2014,Zaleski - Policy Advisor, Energy DepartmentAssessmentAllison Casey Senior

  5. Save Energy on Appliances this Holiday Season | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreakingMay 2015 <Department ofDepartment ofEnergyFebruaryProcessSave Energy

  6. Holiday Shopping and Electric Vehicles | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh School football High SchoolBundlesHistory

  7. LED Holiday Lights: Festive, Safe, and Efficient! | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO2:Introduction to Energy PerformanceJohn CymbalskyKristina Johnson About UsNovember 23, 2010

  8. Pooled ORF Expression Technology (POET) USING PROTEOMICS TO SCREEN POOLS OF OPEN READING FRAMES FOR PROTEIN EXPRESSION*S

    E-Print Network [OSTI]

    Pooled ORF Expression Technology (POET) USING PROTEOMICS TO SCREEN POOLS OF OPEN READING FRAMES developed a pooled ORF expression technology, POET, that uses recombinational cloning and proteomic methods are greatly simplified. Small scale expression and purification of 12 positive clones identified by POET from

  9. On expressive punishment and holisitic desert

    E-Print Network [OSTI]

    Greenblum, Jake

    2009-05-15T23:59:59.000Z

    Expressive theories of punishment incorporate both deontological and consequentialist components. The deontological element claims that punishment expresses the value of both victim and wrongdoer. The consequentialist element claims that punishment...

  10. Towards Efficient Structural Analysis of Mathematical Expressions

    E-Print Network [OSTI]

    Yeung, Dit-Yan

    . Experiments done on some typical mathematical expressions show that our proposed methods can achieve speedup, structural analysis 1 Introduction Many documents in science and engineering disciplines contain mathematical expressions. The input of mathematical expressions into computers is often more difficult than the input

  11. Deformation Expression for Elements of Algebra

    E-Print Network [OSTI]

    H. Omori; Y. Maeda; N. Miyazaki; A. Yoshioka

    2011-04-09T23:59:59.000Z

    The purpose of this paper is to give a notion of deformation of expressions for elements of algebra. Deformation quantization (cf.[BF]) deforms the commutative world to a non-commutative world. However, this involves deformation of expression of elements of algebras even from a commutative world to another commutative world. This is indeed a deformation of expressions for elements of algebra.

  12. Methods for monitoring multiple gene expression

    SciTech Connect (OSTI)

    Berka, Randy (Davis, CA); Bachkirova, Elena (Davis, CA); Rey, Michael (Davis, CA)

    2012-05-01T23:59:59.000Z

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  13. Methods for monitoring multiple gene expression

    DOE Patents [OSTI]

    Berka, Randy; Bachkirova, Elena; Rey, Michael

    2013-10-01T23:59:59.000Z

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  14. Expression equivalence checking using interval analysis

    E-Print Network [OSTI]

    Ghodrat, Mohammad Ali; Givargis, Tony; Nicolau, Alex

    2006-01-01T23:59:59.000Z

    Z. Zhou and W. Burleson, “Equivalence checking of datapathsusing combinational equivalence for extensible processor,”et al. : EXPRESSION EQUIVALENCE CHECKING USING INTERVAL

  15. Gene expression abnormalities in the autistic brain

    E-Print Network [OSTI]

    Chow, Maggie Lok Mun

    2011-01-01T23:59:59.000Z

    lymphoblastoid cell lines (LCLs) derived from individualsby profiling gene expression in LCLs derived from livingsubjects. LCLs derived from (1) three monozygotic twin pairs

  16. LOS ALAMOS NATIONAL LABORATORY - EXPRESS LICENSING PROGRAM

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LOS ALAMOS NATIONAL LABORATORY - EXPRESS LICENSING PROGRAM NON-EXCLUSIVE PATENT LICENSE AGREEMENT This Agreement between Los Alamos National Security, LLC ("LANS"), under its...

  17. Rocky Mountain Power- FinAnswer Express

    Broader source: Energy.gov [DOE]

    Rocky Mountain Power's FinAnswer Express Program provides extensive incentives and for lighting, HVAC, food service, agricultural, and compressed air equipment. Retrofits of facilities and upgrades...

  18. Rocky Mountain Power- FinAnswer Express

    Broader source: Energy.gov [DOE]

    Rocky Mountain Power's FinAnswer Express Program includes incentives and technical assistance for lighting, HVAC and other equipment upgrades that increase energy efficiency and exceed code...

  19. Differential Gene Expression Pre-processing: from CEL files to ExpressionSet

    E-Print Network [OSTI]

    Qiu, Weigang

    different between mutants and wild types Install Libraries and Load Data > source-processing: from CEL files to ExpressionSet Gene Annotation Visualize Expression Profile using Heatmap Produce

  20. Deciding Definability by Deterministic Regular Expressions

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    (DTD) [5] and XML Schema Definition (XSD) [10], both developed by the World Wide Web Consortium, do as well. Intuitively, a regular expression is deterministic if, when reading a word from left to right. For example, the expression (a + b) b(a + b) is not deterministic, because if we read a word that starts

  1. Expression of eukaryotic polypeptides in chloroplasts

    DOE Patents [OSTI]

    Mayfield, Stephen P

    2013-06-04T23:59:59.000Z

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  2. Small Business Express Program (EXP) (Connecticut)

    Broader source: Energy.gov [DOE]

    The Small Business Express Program (EXP) provides loans and grants to small businesses (not more than 100 employees) to spur job creation and growth. The Program provides access to capital through...

  3. Xcel Energy- Express Energy Efficiency Program

    Broader source: Energy.gov [DOE]

    The Express Energy Efficiency Program provides free installation of energy-saving products. This program travels around Wisconsin, community-by-community, to see if they are installing in your...

  4. An expectation model of referring expressions

    E-Print Network [OSTI]

    Kræmer, John, Ph. D. Massachusetts Institute of Technology

    2010-01-01T23:59:59.000Z

    This thesis introduces EMRE, an expectation-based model of referring expressions. EMRE is proposed as a model of non-syntactic dependencies - in particular, discourse-level semantic dependencies that bridge sentence gaps. ...

  5. Dangerous Bodies: Freak Shows, Expression, and Exploitation

    E-Print Network [OSTI]

    Fordham, Brigham A.

    2007-01-01T23:59:59.000Z

    Dangerous Bodies: Freak Shows, Expression, and ExploitationR.I. GEN. LAWS § 11-9-1. DANGEROUS BODIES Rhode Island does161 Id. Id. 165 id. 166 Id. DANGEROUS BODIES This argument

  6. Generating and interpreting referring expressions in context

    E-Print Network [OSTI]

    Smith, Dustin Arthur

    2013-01-01T23:59:59.000Z

    Referring expressions with vague and ambiguous modifiers, such as "a quick visit" and "the big meeting," are difficult for computers to interpret because their meanings are defined in part by context. For the hearer to ...

  7. Identifying expression fingerprints using linguistic information

    E-Print Network [OSTI]

    Uzuner, Ozlem, 1975-

    2005-01-01T23:59:59.000Z

    This thesis presents a technology to complement taxation-based policy proposals aimed at addressing the digital copyright problem. The approach presented facilitates identification of intellectual property using expression ...

  8. Expressive Autonomous Cinematography for Interactive Virtual Environments

    E-Print Network [OSTI]

    Tomlinson, Bill

    Expressive Autonomous Cinematography for Interactive Virtual Environments Bill Tomlinson Synthetic an automatic cinematography system for interactive virtual environments. This system controls a virtual camera this cinematography system with an ethologically-inspired structure of sensors, emotions, motivations, and action

  9. Neurokinin Receptor Expression in the Lymphatic System 

    E-Print Network [OSTI]

    Khade, Parth

    2011-01-11T23:59:59.000Z

    Neurokinin Receptor Expression in the Lymphatic System. (April 2009) Parth Vijay Khade. Department of Biomedical Engineering, Texas A&M University. Research Advisor: Dr. Dave C. Zawieja, Department of Systems Biology and Translational Medicine...

  10. Control of gene expression by cell size

    E-Print Network [OSTI]

    Wu, Chia-Yung

    2010-01-01T23:59:59.000Z

    Polyploidy, increased copy number of whole chromosome sets in the genome, is a common cellular state in evolution, development and disease. Polyploidy enlarges cell size and alters gene expression, producing novel phenotypes ...

  11. Regular expression matching and operational Hayo Thielecke

    E-Print Network [OSTI]

    Thielecke, Hayo

    / 24 #12;The EKW machine e ; k ; w e ; k ; w e1 | e2 ; k ; w e1 ; k ; w e1 | e2 ; k ; w e2 ; k ; w e1 e2 ; k ; w e1 ; e2 :: k ; w e ; k ; w e ; e :: k ; w e ; k ; w ; k ; w a ; k ; a w ; k ; w ; e :: k ; w e ; k ; w 8 / 24 #12;Regular expressions as trees/graphs in memory Expression as trees

  12. Does Standard Cosmology Express Cosmological Principle Faithfully?

    E-Print Network [OSTI]

    Ding-fang Zeng; Hai-jun Zhao

    2005-06-30T23:59:59.000Z

    In 1+1 dimensional case, Einstein equation cannot give us any information on the evolution of the universe because the Einstein tensor of the system is identically zero. We study such a 1+1 dimensional cosmology and find the metric of it according to cosmological principle and special relativity, but the results contradict the usual expression of cosmological principle of standard cosmology. So we doubt in 1+3 dimensional case, cosmological principle is expressed faithfully by standard cosmology.

  13. Low Rates of Expression Profile Divergence in Highly Expressed Genes and Tissue-Specific Genes During Mammalian Evolution

    E-Print Network [OSTI]

    Zhang, Jianzhi

    Low Rates of Expression Profile Divergence in Highly Expressed Genes and Tissue-Specific Genes specificity influence the divergence of expression profiles between orthologous genes. Here we address expression profile change during evolution is negatively correlated with the level of gene expression

  14. DOMESTIC SERVICES FEDERAL EXPRESS UPS US POSTAL SERVICE OTHER

    E-Print Network [OSTI]

    Holliday, Vance T.

    SERVICES FEDERAL EXPRESS UPS US POSTAL SERVICE OTHER ___ First Overnight ___ Air ___ Regular Post _____________________________________ ___ Express Saver ___ Ground FEDERAL EXPRESS UPS US POSTAL SERVICE OTHER ___ Int. First ___ Air ___ Air Mail. Economy _____________________________________ Fill in complete mailing address. FedEx and UPS

  15. albumin gene expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biology, and multiple orthogonal gene switches are needed Zhao, Huimin 223 Metabolic load and heterologous gene expression CiteSeer Summary: The expression of a foreign...

  16. abnormal phenotypic expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D.R. and Linkenauger, S.A. (in press). Perception viewed as a phenotypic expression: Perception Viewed as a Phenotypic Expression Correspondence: Dennis Proffitt Department of...

  17. EIS-0447: Champlain Hudson Power Express Transmission Line Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    47: Champlain Hudson Power Express Transmission Line Project, New York EIS-0447: Champlain Hudson Power Express Transmission Line Project, New York Summary This EIS evaluated the...

  18. aortic enos expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the rate of gene sequence evolution has been well studied, the rate of gene expression evolution is poorly understood. In particular, it is unclear whether the gene expression...

  19. Gene Expression Profiling of 3T3-L1 Adipocytes Expressing the Mitochondrial Uncoupling Protein 1 (UCP1) 

    E-Print Network [OSTI]

    Senocak, Fatih

    2006-07-11T23:59:59.000Z

    changes in energy metabolism in white adipocytes. Specifically, we used DNA microarrays to characterize the changes in white adipocyte gene expression upon UCP1 expression and determine the extent to which UCP1 expressing white adipocytes emulate brown...

  20. The in vitro characterization of heterologously expressed enzymes to inform in vivo biofuel production optimization

    E-Print Network [OSTI]

    Garcia, David Ernest

    2013-01-01T23:59:59.000Z

    The expression, purification, and kinetic characterizationThe expression, purification, and kinetic characterizationThe expression, purification, and kinetic characterization

  1. EXPRESS 2008 Expressiveness in Concurrency 15th int. workshop Security Abstractions and Intruder Models

    E-Print Network [OSTI]

    Bugliesi, Michele

    along these lines [5], we investigate the expressive power of a core set of security and network to secure communications by hiding them on private channels. A more recent line of research [2 of our approach by investigating the expressive power of our security and network abstractions

  2. Perceptual Smoothness of Tempo in Expressively Performed Simon Dixon

    E-Print Network [OSTI]

    Dixon, Simon

    a wide range of expressive timing deviations (Large & Kolen, 1994; Goto & Muraoka, 1995; Dixon, 2001a

  3. TrekISM Issue 67

    E-Print Network [OSTI]

    1990-01-01T23:59:59.000Z

    . Write to Vulkon, PO Box 786, Hollywood FL 33022-0786. STAR-FEST - March 22-24, Holiday Inn, Denver CO. Guests: Leonard Nimoy, Colm Meany. Write to: Starland, 8200 East Pacific Place, #307, Denver CO 80231 DREAMWERKS - April 6-7, Cleveland Guest... on keeping my medicines straight -- the cold that arrived in time for the wedding stubbornly refused to go away, and decided to stay as bronchitis. I have to remember to take the antibiotic one hour before eating, or 3 hours after, and to drink lots...

  4. Report on the technical workshop on WTI incinerator risk issues. Held in Washington, DC on December 8-9, 1993

    SciTech Connect (OSTI)

    Not Available

    1993-12-01T23:59:59.000Z

    The report includes information and materials from a peer review workshop organized by EPA's Risk Assessment Forum (RAF) for the Office of Solid Waste and Emergency Response and Region 5. The meeting was held in Washington, DC, at the Holiday Inn Capitol on December 8-9, 1993. The subject of the peer review was a draft project plan prepared by EPA Region 5 for assessing risk at an incinerator operated by Waste Technologies Industries (WTI) in East Liverpool, Ohio. The peer review panel was convened to evaluate the project plan as the scientific foundation for a risk assessment, which will be used in setting final permit conditions for the WTI facility.

  5. Gene Expression in the Stallion Testes

    E-Print Network [OSTI]

    Laughlin, Andy M.

    2011-08-08T23:59:59.000Z

    genes were differentially expressed (122 in fertile tissue, 111 in subfertile tissue). Of these, phosphodiesterase 3B (PDE3B), steroidogenic acute regulatory (StAR) protein, and outer dense fiber of sperm tails 2 (ODF2) mRNAs, were localized...

  6. TWO THEORIES FOR COMPUTING THE LOGICAL FORM OF MASS EXPRESSIONS Francis Jeffry Pelletier

    E-Print Network [OSTI]

    There are various difficulties in accomodating the traditional logical expressions into larger expressions

  7. Gene expression profiles in irradiated cancer cells

    SciTech Connect (OSTI)

    Minafra, L.; Bravatà, V.; Russo, G.; Ripamonti, M.; Gilardi, M. C. [IBFM CNR - LATO, Cefalù, Segrate (Italy)] [IBFM CNR - LATO, Cefalù, Segrate (Italy)

    2013-07-26T23:59:59.000Z

    Knowledge of the molecular and genetic mechanisms underlying cellular response to radiation may provide new avenues to develop innovative predictive tests of radiosensitivity of tumours and normal tissues and to improve individual therapy. Nowadays very few studies describe molecular changes induced by hadrontherapy treatments, therefore this field has to be explored and clarified. High-throughput methodologies, such as DNA microarray, allow us to analyse mRNA expression of thousands of genes simultaneously in order to discover new genes and pathways as targets of response to hadrontherapy. Our aim is to elucidate the molecular networks involved in the sensitivity/resistance of cancer cell lines subjected to hadrontherapy treatments with a genomewide approach by using cDNA microarray technology to identify gene expression profiles and candidate genes responsible of differential cellular responses.

  8. Regulation of methane genes and genome expression

    SciTech Connect (OSTI)

    John N. Reeve

    2009-09-09T23:59:59.000Z

    At the start of this project, it was known that methanogens were Archaeabacteria (now Archaea) and were therefore predicted to have gene expression and regulatory systems different from Bacteria, but few of the molecular biology details were established. The goals were then to establish the structures and organizations of genes in methanogens, and to develop the genetic technologies needed to investigate and dissect methanogen gene expression and regulation in vivo. By cloning and sequencing, we established the gene and operon structures of all of the “methane” genes that encode the enzymes that catalyze methane biosynthesis from carbon dioxide and hydrogen. This work identified unique sequences in the methane gene that we designated mcrA, that encodes the largest subunit of methyl-coenzyme M reductase, that could be used to identify methanogen DNA and establish methanogen phylogenetic relationships. McrA sequences are now the accepted standard and used extensively as hybridization probes to identify and quantify methanogens in environmental research. With the methane genes in hand, we used northern blot and then later whole-genome microarray hybridization analyses to establish how growth phase and substrate availability regulated methane gene expression in Methanobacterium thermautotrophicus ?H (now Methanothermobacter thermautotrophicus). Isoenzymes or pairs of functionally equivalent enzymes catalyze several steps in the hydrogen-dependent reduction of carbon dioxide to methane. We established that hydrogen availability determine which of these pairs of methane genes is expressed and therefore which of the alternative enzymes is employed to catalyze methane biosynthesis under different environmental conditions. As were unable to establish a reliable genetic system for M. thermautotrophicus, we developed in vitro transcription as an alternative system to investigate methanogen gene expression and regulation. This led to the discovery that an archaeal protein, designated TFE, that had sequences in common with the eukaryotic general transcription factor TFIIE, stimulated archaeal transcription initiation and that the archaeal TATA-box binding protein (TBP) remained attached to the promoter region whereas the transcription factor TFB dissociated from the template DNA following initiation. DNA sequences that directed the localized assembly of archaeal histones into archaeal nucleosomes were identified, and we established that transcription by an archaeal RNA polymerase was slowed but not blocked by archaeal nucleosomes. We developed a new protocol to purify archaeal RNA polymerases and with this enzyme and additional improvements to the in vitro transcription system, we established the template requirements for archaeal transcription termination, investigated the activities of proteins predicted to be methane gene regulators, and established how TrpY, a novel archaeal regulator of expression of the tryptophan biosynthetic operon functions in M. thermautotrophicus. This also resulted in the discovery that almost all M. thermautotrophicus mutants isolated as spontaneously resistant to 5-methyl tryptophan (5MTR) had mutations in trpY and were therefore 5MTR through de-repressed trp operon expression. This established a very simple, practical procedure to determine and quantify the DNA sequence changes that result from exposure of this Archaeon to any experimental mutagenesis protocol. Following the discovery that the Thermococcus kodakaraensis was amenable to genetic manipulation, we established this technology at OSU and subsequently added plasmid expression, a reporter system and additional genetic selections to the T. kodakaraensis genetic toolbox. We established that transcription and translation are coupled in this Archaeon, and by combining in vitro transcription and in vivo genetics, we documented that both TFB1 and TFB2 support transcription initiation in T. kodakaraensis. We quantified the roles of ribosome binding sequences and alternative initiation codons in translation initiation, established that polarity e

  9. Kinetic decoupling of WIMPs: analytic expressions

    E-Print Network [OSTI]

    Visinelli, Luca

    2015-01-01T23:59:59.000Z

    We present a general expression for the values of the average kinetic energy and of the temperature of kinetic decoupling of a WIMP, valid for any cosmological model. We show an example of the usage of our solution when the Hubble rate has a power-law dependence on temperature, and we show results for the specific cases of kination cosmology and low- temperature reheating cosmology.

  10. Modeling of Distributed Systems by Concurrent Regular Expressions

    E-Print Network [OSTI]

    Garg, Vijay

    Modeling of Distributed Systems by Concurrent Regular Expressions Vijay K. Garg Department of Electrical and Computer Engineering, University of Texas, Austin, TX 78712 We propose an algebraic model called concurrent regular expressions for modeling and anal- ysis of distributed systems

  11. Modeling of Distributed Systems by Concurrent Regular Expressions

    E-Print Network [OSTI]

    Garg, Vijay

    Modeling of Distributed Systems by Concurrent Regular Expressions Vijay K. Garg Department of Electrical and Computer Engineering, University of Texas, Austin, TX 78712 We propose an algebraic model called concurrent regular expressions for modeling and anal­ ysis of distributed systems

  12. Ectopic ERK Expression Induces Phenotypic Conversion of C10 Cells and Alters DNA Methyltransferase Expression

    SciTech Connect (OSTI)

    Sontag, Ryan L.; Weber, Thomas J.

    2012-05-04T23:59:59.000Z

    In some model systems constitutive extracellular signal regulated kinase (ERK) activation is sufficient to promote an oncogenic phenotype. Here we investigate whether constitutive ERK expression influences phenotypic conversion in murine C10 type II alveolar epithelial cells. C10 cells were stably transduced with an ERK1-green fluorescent protein (ERK1-GFP) chimera or empty vector and ectopic ERK expression was associated with the acquisition of soft agar focus-forming potential in late passage, but not early passage cells. Late passage ERK1-GFP cells exhibited a significant increase in the expression of DNA methyl transferases (DNMT1 and 3b) and a marked increase in sensitivity to 5-azacytidine (5-azaC)-mediated toxicity, relative to early passage ERK1-GFP cells and vector controls. The expression of xeroderma pigmentosum complementation group A (XPA) and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) were significantly increased in late passage cells, suggesting enhanced DNA damage recognition and repair activity which we interpret as a reflection of genomic instability. Phospho-ERK levels were dramatically decreased in late passage ERK1-GFP cells, relative to early passage and vector controls, and phospho-ERK levels were restored by treatment with sodium orthovanadate, indicating a role for phosphatase activity in this response. Collectively these observations suggest that ectopic ERK expression promotes phenotypic conversion of C10 cells that is associated with latent effects on epigenetic programming and phosphatase activities.

  13. Interactive 3D Gene Expression Viewer Victor E. Gerth*

    E-Print Network [OSTI]

    Vize, Peter D.

    Interactive 3D Gene Expression Viewer Victor E. Gerth* University of Calgary, Department. The Interactive Gene Expression viewer provides a way to view spatial relationships between different gene expression patterns and anatomic features. Web based 3D enabled technologies such as the Interactive Gene

  14. Generating and Interpreting Referring Expressions in Dustin Arthur Smith

    E-Print Network [OSTI]

    Referring Expressions in Context by Dustin Arthur Smith Submitted to the Program of Media Arts and Sciences, MIT Media Lab #12;Generating and Interpreting Referring Expressions in Context by Dustin Arthur SmithGenerating and Interpreting Referring Expressions in Context by Dustin Arthur Smith B.S., Wake

  15. Comparing the Expressive Power of Access Control Mahesh V. Tripunitara

    E-Print Network [OSTI]

    Li, Ninghui

    Comparing the Expressive Power of Access Control Models Mahesh V. Tripunitara tripunit the expressive power of access control models is rec- ognized as a fundamental problem in computer security the expressive power of access control models into a single context and to compare such models to one another

  16. TECHNICAL ADVANCE Spatial control of transgene expression in rice

    E-Print Network [OSTI]

    Haseloff, Jim

    spatial control of transgene expression in all organs of the model monocotyledonous species rice (Oryza. In particular, spatial control of trans- gene expression allowing the manipulation of gene expres- sionTECHNICAL ADVANCE Spatial control of transgene expression in rice (Oryza sativa L.) using the GAL4

  17. Probabilistic Models for Collecting, Analyzing, and Modeling Expression Data

    E-Print Network [OSTI]

    ;Keywords: genomics, gene expression, gene regulation, microarray, RNA-Seq, transcriptomics, error to integrate expression and interaction data in order to predict targets and networks regulated by microRNAs. Combined, the methods developed in this thesis provide a solution to the pipeline of expression analysis

  18. CSP is expressive enough for A.W. Roscoe

    E-Print Network [OSTI]

    Oxford, University of

    CSP is expressive enough for A.W. Roscoe Oxford University Computing Laboratory {Bill.Roscoe@comlab.ox.ac.uk} Abstract. Recent results show that Hoare's CSP, augmented by one additional operator, can express every operator whose operational semantics are expressible in a new notation and are therefore "CSP

  19. Reduced expression of Autographa californica nucleopolyhedrovirus ORF34, an essential gene, enhances heterologous gene expression

    SciTech Connect (OSTI)

    Salem, Tamer Z. [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States) [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States); Department of Microbial Molecular Biology, AGERI, Agricultural Research Center, Giza 12619 (Egypt); Division of Biomedical Sciences, Zewail University, Zewail City of Science and Technology, Giza 12588 (Egypt); Zhang, Fengrui [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States)] [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States); Thiem, Suzanne M., E-mail: smthiem@msu.edu [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States); Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824 (United States)

    2013-01-20T23:59:59.000Z

    Autographa californica multiple nucleopolyhedrovirus ORF34 is part of a transcriptional unit that includes ORF32, encoding a viral fibroblast growth factor (FGF) and ORF33. We identified ORF34 as a candidate for deletion to improve protein expression in the baculovirus expression system based on enhanced reporter gene expression in an RNAi screen of virus genes. However, ORF34 was shown to be an essential gene. To explore ORF34 function, deletion (KO34) and rescue bacmids were constructed and characterized. Infection did not spread from primary KO34 transfected cells and supernatants from KO34 transfected cells could not infect fresh Sf21 cells whereas the supernatant from the rescue bacmids transfection could recover the infection. In addition, budded viruses were not observed in KO34 transfected cells by electron microscopy, nor were viral proteins detected from the transfection supernatants by western blots. These demonstrate that ORF34 is an essential gene with a possible role in infectious virus production.

  20. Expression of multiple proteins in transgenic plants

    DOE Patents [OSTI]

    Vierstra, Richard D. (Madison, WI); Walker, Joseph M. (Madison, WI)

    2002-01-01T23:59:59.000Z

    A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.

  1. MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS

    E-Print Network [OSTI]

    Fiedler, Stephanie Deanne

    2008-01-01T23:59:59.000Z

    ] primary miRNA (pri-miRNA) [32, 33]. Pri-miRNAs are cleaved by the RNase III endonuclease Drosha which works in combination with the co-factor DGCR8 to produce a ~60-70 nucleotide (nt) stem-loop intermediate precursor miRNA (pre-miRNA) that contains a... spines [70] via a microtubule based mechanism [71] which promotes the retraction of the cell membrane [66]. In addition to neuronal tissue, p250GAP has also been shown to be expressed in the human ovary [72], and the worm homolog (RRC-1) is known to co...

  2. Express Package Shipping Services | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist. Category UC-l 1,EnergyExploring the Nature ofExpress Package

  3. Peroxidase gene expression during tomato fruit ripening

    SciTech Connect (OSTI)

    Biggs, M.S.; Flurkey, W.H.; Handa, A.K.

    1987-04-01T23:59:59.000Z

    Auxin oxidation has been reported to play a critical role in the initiation of pear fruit ripening and a tomato fruit peroxidase (POD) has been shown to have IAA-oxidase activity. However, little is known about changes in the expression of POD mRNA in tomato fruit development. They are investigating the expression of POD mRNA during tomato fruit maturation. Fruit pericarp tissues from six stages of fruit development and ripening (immature green, mature green, breaker, turning, ripe, and red ripe fruits) were used to extract poly (A)/sup +/ RNAs. These RNAs were translated in vitro in a rabbit reticulocyte lysate system using L-/sup 35/S-methionine. The /sup 35/S-labeled products were immunoprecipitated with POD antibodies to determine the relative proportions of POD mRNA. High levels of POD mRNA were present in immature green and mature green pericarp, but declined greatly by the turning stage of fruit ripening. In addition, the distribution of POD mRNA on free vs bound polyribosomes will be presented, as well as the presence or absence of POD mRNA in other tomato tissues.

  4. Analysis of FRUITFULL promoter motifs and their influence on valve expression during fruit development

    E-Print Network [OSTI]

    Woods, Jennifer Carol

    2010-01-01T23:59:59.000Z

    motifs and their influence on valve expression during fruitappear WT-like with only valve expression remaining (Fig.motifs and their influence on valve expression during fruit

  5. December 2010: Happy Holidays! We, in the School of Engineering, wish you all the best the season has to offer. Click here for our holiday greeting.

    E-Print Network [OSTI]

    Holliday, JoAnne

    Meyyappan. More information. James Foley '68 honored by NCEES: Congratulations to civil engineering alumnus James Foley of Saratoga, CA, who was awarded the NCEES Distinguished Service Award for his dedicated service to the engineering and surveying professions. Foley has held numerous leadership positions

  6. Tax Information Publication -2014 New Energy Star and WaterSense Products Sales Tax Holiday 1 2014 Sales Tax Holiday for

    E-Print Network [OSTI]

    Florida, University of

    ,599 for the second refrigerator are subject to tax. Installation Charges When a qualifying energy-efficient product

  7. Repressor-mediated tissue-specific gene expression in plants

    DOE Patents [OSTI]

    Meagher, Richard B. (Athens, GA); Balish, Rebecca S. (Oxford, OH); Tehryung, Kim (Athens, GA); McKinney, Elizabeth C. (Athens, GA)

    2009-02-17T23:59:59.000Z

    Plant tissue specific gene expression by way of repressor-operator complexes, has enabled outcomes including, without limitation, male sterility and engineered plants having root-specific gene expression of relevant proteins to clean environmental pollutants from soil and water. A mercury hyperaccumulation strategy requires that mercuric ion reductase coding sequence is strongly expressed. The actin promoter vector, A2pot, engineered to contain bacterial lac operator sequences, directed strong expression in all plant vegetative organs and tissues. In contrast, the expression from the A2pot construct was restricted primarily to root tissues when a modified bacterial repressor (LacIn) was coexpressed from the light-regulated rubisco small subunit promoter in above-ground tissues. Also provided are analogous repressor operator complexes for selective expression in other plant tissues, for example, to produce male sterile plants.

  8. ESCO EXPRESSION OF INTEREST IN NOTICE OF OPPORTUNITY | Department...

    Broader source: Energy.gov (indexed) [DOE]

    essionofinterestform.docx More Documents & Publications LETTER TO SUCCESSFUL COMPANY OR ESCO ESCO EXPRESSION OF INTEREST EVALUATION WORKSHEET ESPC ENABLE PRELIMINARY LETTER TO...

  9. Snohomish County PUD No 1- Solar Express Rebate Program

    Broader source: Energy.gov [DOE]

    In March 2009, Snohomish County PUD introduced the Solar Express Program. This program provides rebates to support residential and commercial installations of solar photovoltaics (PV) and solar...

  10. assess expression profiles: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the application of a novel clustering algorithm, Super-Paramagnetic Clustering (SPC) to analysis of gene expression profiles that were generated recently during a study of...

  11. Genomics, Gene Expression and Other Studies in Soybean Rust

    E-Print Network [OSTI]

    Posada-Buitrago, Martha Lucia

    2005-01-01T23:59:59.000Z

    Joint Genome Institute Genomics, Gene Expression and otherRust Martha Lucía Posada-Buitrago Ph.D Genomics DivisionEvolutionary Genomics DOE- Joint Genome Institute Lawrence

  12. acc synthase expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    corroborate asymmetry of catalysis in F0F1-ATP synthase. Zarrabi, Nawid; Diez, Manuel; Graeber, Peter; Wrachtrup, Joerg; Boersch, Michael 2007-01-01 202 expression? Environments...

  13. acid decarboxylase expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the expressions. Anna Sthl; Petra Sundstrm; Kristina Hk 2005-01-01 180 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  14. acid oxidase expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the expressions. Anna Sthl; Petra Sundstrm; Kristina Hk 2005-01-01 252 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  15. Electrical and Electrothermal Transport Properties of n- and p-type InN

    E-Print Network [OSTI]

    Miller, Nathaniel Reed

    2010-01-01T23:59:59.000Z

    Berlin , 265 (1823). [166] Peltier, J. Ann. Chim. Phys 56,which is also known as the Peltier coefficient. Note that p-en.wikipedia.org/wiki/Peltier Open source images under

  16. Inn vati ns at EECS: Techn l gy f r a gl bal future

    E-Print Network [OSTI]

    California at Irvine, University of

    with Resiliency and Efficiency Lab) & BWRC (Berkeley Wireless Research Center) · Customized 3D Printed Implants - Peter Bailis, AMPLab (Algorithms, Machines, and People Laboratory) · Raven: An Energy Wireless Research Center) & E3S (Center for Energy Efficient Electronics Science) · Occupant Detection

  17. Materials Data on InN (SG:186) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  18. Materials Data on Lu3InN (SG:221) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02T23:59:59.000Z

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. Materials Data on InN (SG:186) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02T23:59:59.000Z

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. 8/20/2010 1 C380 Med Inn, SPC 5838

    E-Print Network [OSTI]

    Shyy, Wei

    be noted that medical qualification for respirator use is dependent upon proper fit testing and instruction regarding use and maintenance of respiratory equipment. Clinician signature Date Name/UM Registration # #12;

  1. Lois Curfman McInnes, 2011 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNucleartearing modeTissueofLogisticLois

  2. Konocti Harbor Inn Pool & Spa Low Temperature Geothermal Facility | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to: navigation, search GEOTHERMALTexas: Energy Resources JumpKobeKonarkaEnergy

  3. The Saratoga Inn Pool & Spa Low Temperature Geothermal Facility | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries PvtStratosolarTharaldson Ethanol LLC Jump to:Uncertainty of1801Finance

  4. River Inn Natural Hot Spring Pool & Spa Low Temperature Geothermal Facility

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries Pvt Ltd Jump to: navigation,Maze -RichtonMissouri: EnergyRitzville,Edge,| Open

  5. Riverside Inn Pool & Spa Low Temperature Geothermal Facility | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries Pvt Ltd Jump to: navigation,Maze -RichtonMissouri:Park,Energy

  6. Furnace Creek Inn Pool & Spa Low Temperature Geothermal Facility | Open

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump1946865°, -86.0529604°Wisconsin: Energy ResourcesFuquay-Varina,

  7. Geothermal Direct Use Technology & Marketplace Hilton Garden Inn Pittsburgh/Southpointe

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.Newof EnergyFundingGeneGenomeGeoffreyGeorgeDirect Use

  8. Heterocyst Morphogenesis and Gene Expression in Anabaena sp. PCC 7120 

    E-Print Network [OSTI]

    Mella Herrera, Rodrigo Andres

    2010-10-12T23:59:59.000Z

    , diazotrophic grow, and heterocyst morphogenesis. In our studies we characterized the expression of sigma factors genes in Anabaena PCC 7120 during heterocyst differentiation, and we found that the expression of sigC, sigG and sigE is localized primarily...

  9. Explicit Expressions for Moments of Log Normal Order Statistics

    E-Print Network [OSTI]

    Sidorov, Nikita

    Explicit Expressions for Moments of Log Normal Order Statistics Saralees Nadarajah First version: 31 December 2006 Research Report No. 23, 2006, Probability and Statistics Group School of Mathematics, The University of Manchester #12;Explicit Expressions for Moments of Log Normal Order Statistics by Saralees

  10. Mining Complex Boolean Expressions for Sequential Equivalence Checking

    E-Print Network [OSTI]

    Ramakrishnan, Naren

    Mining Complex Boolean Expressions for Sequential Equivalence Checking Neha Goel, Michael S. Hsiao-flops in a sequential circuit for sequential equivalence checking. In contrast to traditional learning methods, our be arbitrary boolean expressions and can thus prune a large don't care space during equivalence checking

  11. NEUROSYSTEMS Connexin26 expression in brain parenchymal cells

    E-Print Network [OSTI]

    Rash, John E.

    are known to express the gap junction forming proteins connexin30 (Cx30) and connexin43 (Cx43), but it has remained controversial whether these cells also express connexin26 (Cx26). To investigate this issue with targeted deletion of Cx26 in neuronal and glial cells (Cx26fl / fl:Nestin-Cre mice). The Cx26 antibodies

  12. On the Expressive Power of the Unary Transformation Model

    E-Print Network [OSTI]

    Sandhu, Ravi

    we analyze the expressive power of a family of access control models called transformation models [10On the Expressive Power of the Unary Transformation Model Ravi S. Sandhu and Srinivas Ganta Center. The Transformation Model (TRM) was recently introduced [10] in the literature by Sandhu and Ganta. TRM is based

  13. Computational Molecular Biology of Genome Expression and Regulation

    E-Print Network [OSTI]

    Computational Molecular Biology of Genome Expression and Regulation Michael Q. Zhang, Ph.D. Cold in genome expression and regulation network in which better modern statistical and machine learning referring to the cellular processes that lead to pro- tein production, is controlled and regulated

  14. Systematic Dissection of the Determinants of HIV Expression Noise

    E-Print Network [OSTI]

    Foley, Jonathan Emmett

    2013-01-01T23:59:59.000Z

    six days post infection, gene expression was stimulated with 20 ng/ ml tumor necrosis factor-oc (TNF-oc, Sigma-six days post infection, gene expression was stimulated with 20 ng/ml tumor necrosis factor-on (TNF-(1, 29 Sigma-

  15. On the expressiveness of CSP A.W. Roscoe

    E-Print Network [OSTI]

    Oxford, University of

    On the expressiveness of CSP A.W. Roscoe February 16, 2011 Abstract We define "CSP express every operator of Hoare's CSP. Furthermore we show that every op- erator with CSP-like operational semantics can be simulated in CSP with the addition of an exception-throwing operator P A Q in which any

  16. Diversity of gene expression in adenocarcinoma of the lung

    E-Print Network [OSTI]

    Botstein, David

    Diversity of gene expression in adenocarcinoma of the lung Mitchell E. Garber*, Olga G. Troyanskaya Contributed by David Botstein, September 21, 2001 The global gene expression profiles for 67 human lung tumors pathologic analysis. Four main histologic subtypes of lung cancer are regularly distinguished by tumor

  17. TECHNICAL ADVANCE BAR expressolog identification: expression profile similarity

    E-Print Network [OSTI]

    Toronto, University of

    TECHNICAL ADVANCE BAR expressolog identification: expression profile similarity ranking with the highest sequence similarity do not necessarily exhibit the highest expression pattern similarity. Other-annotation or more specific annotation. Ultimately, it is envisaged that this pipeline will aid in improvement

  18. Diurnal oscillation of SBE expression in sorghum endosperm

    SciTech Connect (OSTI)

    Sun, Chuanxin; Mutisya, J.; Rosenquist, S.; Baguma, Y.; Jansson, C.

    2009-01-15T23:59:59.000Z

    Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum was cloned and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was expressed also in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15-24 days after pollination. This is different from barley and maize where SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.

  19. Combined hairpin-antisense compositions and methods for modulating expression

    DOE Patents [OSTI]

    Shanklin, John; Nguyen, Tam

    2014-08-05T23:59:59.000Z

    A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

  20. Sales Tax Exemption for Energy-Efficient Products (Sales Tax Holiday)

    Broader source: Energy.gov [DOE]

    Virginia allows a four-day sales tax exemption on Energy Star products of $2,500 or less per product, purchased for non-commercial home or personal use. Beginning in 2007, the 100% exemption from...

  1. Sales Tax Exemption for Energy-Efficient Products (Sales Tax Holiday) (Georgia))

    Broader source: Energy.gov [DOE]

    Georgia allows an annual state and local sales tax exemption on Energy Star products of $1,500 or less per product, purchased for non-commercial home or personal use.The 100% exemption from the...

  2. They're Here! Winter, Holidays, and the New Year. How Will You...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    quick energy-related reminders for the next few weeks: Celebrate Efficiently As you're running through your to-do list, don't forget to think about how you can save energy during...

  3. Effect of iron concentration on growth and phylloclade edge yellowing of holiday cactus

    E-Print Network [OSTI]

    Ramirez, Dario

    1996-01-01T23:59:59.000Z

    with applied Fe concentration, and if temperature and pH of the growing media affected Fe uptake. In two separate studies, rooted cuttings of Schlumbergera 'White Christmas,"Twilight Tangerine,"Christmas Charm,'and 'Lavender Doll'were placed in either a peat...

  4. What Do You Think of Your LED Holiday Lights? | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA group currentBradley Nickell DirectorThe&Management AlertWhat Are Your

  5. EECBG Success Story: North Pole's Holiday Wish for an Energy Efficient 2012

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S.ContaminationJulySavannah River SiteDepartment ofDepartmentPower || Department

  6. Top 5 Things You Didn't Know About Holiday Lights | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron Spin Transition in2, 2003Tool of tomorrow today ToolTopWind Power1ofTop5

  7. Effect of iron concentration on growth and phylloclade edge yellowing of holiday cactus 

    E-Print Network [OSTI]

    Ramirez, Dario

    1996-01-01T23:59:59.000Z

    with applied Fe concentration, and if temperature and pH of the growing media affected Fe uptake. In two separate studies, rooted cuttings of Schlumbergera 'White Christmas,"Twilight Tangerine,"Christmas Charm,'and 'Lavender Doll'were placed in either a peat...

  8. Buying an Appliance this Holiday Season? ENERGY STAR Products will Save You

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the YouTube platformBuilding Removal Ongoing atGreenhouse GasesRespond to anSpecifiers

  9. Question of the Week: What Are You Doing to Save Energy this Holiday

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China 2015of 2005UNS Electric,RM ExitProperty Transfer or37 PMTheTheSeason? | Department

  10. Reduce Waste and Save Energy this Holiday Season | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China 2015of 2005UNS Electric,RM ExitPropertySeptemberof Energy Patricia A.

  11. Buying an Appliance this Holiday Season? ENERGY STAR Products will Save You

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you sure you want toworldPower 2010 1AAcquisition »Department ofMoney and

  12. Cut Gas Costs This Holiday Traveling Season with Three Easy Tips |

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you sure you want toworldPower 2010Conferencing andContactsCriminal

  13. How do You Save Energy During the Holidays? | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy ChinaofSchaefer To:Department ofOral Testimony of SecretaryBetween family vacations,

  14. North Pole's Holiday Wish for An Energy Efficient 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn'tOrigin of Contamination in ManyDepartment of Energy North AmericanDepartmentLauderdale

  15. Y-12 helps more than 600 kids for the holidays | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA groupTuba City,EnrichedSupplemental DirectivesWork Schedules

  16. North Pole's Holiday Wish for An Energy Efficient 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreaking ofOilNEW HAMPSHIREofNewsletter NewsletterGeneralofandDepartmentDepartment

  17. Video e-card offers holiday greetings from everyone at PPPL | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron SpinPrincetonUsing Maps1 - USAFofEmail Mr. William HirstVictor

  18. Your Holidays ... Brought to You by Fuel Cells | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: The Future of Bad Cholesterol Your Density Isn't Your Destiny: The Future of

  19. EECBG Success Story: North Pole's Holiday Wish for an Energy Efficient 2012

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny:Revised Finding of98-F, Western SystemsDepartment ofResults || Department of

  20. They're Here! Winter, Holidays, and the New Year. How Will You Save Energy?

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn April 23,EnergyChicopeeTechnology PerformanceDepartmentfor ImprovingA digital|

  1. Y-12 employees help make holiday merrier for many | Y-12 National Security

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengtheningWildfires may contribute moredetectionSecurity

  2. They're Here! Winter, Holidays, and the New Year. How Will You Save Energy?

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742 33Frequently AskedEnergyIssuesEnergy SolarRadioactive LiquidSavings forinitial In thei2013DRAFT|

  3. South Carolina Community Lights Up the Season with Energy-Efficient Holiday

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdfBreakingMayDepartment of EnergySite ScreeningSound Oil CompanySelectionLights |

  4. How Do You Save Money on Summer Holidays? | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed offOCHCO2: FinalOffers3.pdf0-45.pdf0 BudgetGoalsHealth and(JuneEnergyAre YouYour FamilyDo You

  5. It's Finally Time to Think about the Holidays! | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy ChinaofSchaeferApril 1, 1999 InspectionVolunteers from the WaverlySanDepartment ofUsing

  6. antigen expression varies: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ikeda; Kazuo Katoh; Hiroyuki Koshimoto; Yoshihiro Yoshihara; Kensaku Mori 1993-01-01 10 NANO EXPRESS Open Access AuPd core-shell nanoparticles with varied hollow Energy Storage,...

  7. ORIGINAL PAPER Identifying differentially expressed genes in human acute leukemia

    E-Print Network [OSTI]

    Gu, Xun

    ORIGINAL PAPER Identifying differentially expressed genes in human acute leukemia and mouse brain the experimental-wise false discovery rate. A human acute leukemia dataset corrected from 38 leukemia patients

  8. adipose tissue expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the expression of the UCP1 gene 293 D. Norepinephrine is an apoptosis inhibitor in brown adipocytes 294 IV. How Significant Is Brown Adipose Tissue? 295 A. Parameters of...

  9. ankara vaccine expressing: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    21 22 23 24 25 Next Page Last Page Topic Index 1 Role of Vaccination in the Control of Turkey Coccidiosis: Vaccine Associated Oocyst Shedding, Lesions, and Mucosal Gene Expression...

  10. Data mining techniques for large-scale gene expression analysis

    E-Print Network [OSTI]

    Palmer, Nathan Patrick

    2011-01-01T23:59:59.000Z

    Modern computational biology is awash in large-scale data mining problems. Several high-throughput technologies have been developed that enable us, with relative ease and little expense, to evaluate the coordinated expression ...

  11. Computational discovery of gene modules, regulatory networks and expression programs

    E-Print Network [OSTI]

    Gerber, Georg Kurt, 1970-

    2007-01-01T23:59:59.000Z

    High-throughput molecular data are revolutionizing biology by providing massive amounts of information about gene expression and regulation. Such information is applicable both to furthering our understanding of fundamental ...

  12. aging decreases expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    interacting sensory stimuli... Duncan, Nikki 2013-03-13 35 Analysis of Telomere Length in the Aged mdx Diaphragm with and without Transgenic IGF-I Expression CiteSeer...

  13. Compiling Path Expressions into VLSI Circuits `I`. S. Annntharaman

    E-Print Network [OSTI]

    Clarke, Edmund M.

    -timed. Ncvcrthdcss. UK circuits produced by our construction have area propordonal to N slog(N) whcrc N is the total expression: path R, + Wend, path R, + Wend. `fhc first path cxprcssion prohibits a read operation

  14. MicroRNAs can generate thresholds in target gene expression

    E-Print Network [OSTI]

    Mukherji, Shankar

    MicroRNAs (miRNAs) are short, highly conserved noncoding RNA molecules that repress gene expression in a sequence-dependent manner. We performed single-cell measurements using quantitative fluorescence microscopy and flow ...

  15. INVITED REVIEW: TARGETING AND EXPRESSION OF ANTIGENIC PROTEINS IN TRANSGENIC

    E-Print Network [OSTI]

    Korban, Schuyler S.

    OF EDIBLE ORAL VACCINES SCHUYLER S. KORBAN* Department of Natural Resources & Environmental Sciences, 310 of edible oral subunit vaccines is a promising application of biotechnology. Efforts to enhance expression

  16. adaptor protein expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cells before meiosis. After Smith, Steven E. 175 Reduced 293T cell susceptibility to acrolein due to aldose reductase-like-1 protein expression CiteSeer Summary: Acrolein is a...

  17. activation protein expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the relative rates of protein synthesis 98 Reduced 293T cell susceptibility to acrolein due to aldose reductase-like-1 protein expression CiteSeer Summary: Acrolein is a...

  18. aldose reductase expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    21 22 23 24 25 Next Page Last Page Topic Index 1 Reduced 293T cell susceptibility to acrolein due to aldose reductase-like-1 protein expression CiteSeer Summary: Acrolein is a...

  19. Written emotional expression: Effects on weight loss and maintenance

    E-Print Network [OSTI]

    Hockemeyer, Jill Renee

    2007-10-16T23:59:59.000Z

    the negative effects of distress on weight loss and maintenance through improved emotional self-regulatory processes. This study examined the effects of expressive writing on weight regain, stress, and emotional eating among individuals who have recently lost...

  20. Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

    E-Print Network [OSTI]

    Halazonetis, Thanos

    , microalgae, Chlamydomonas rein- hardtii. Summary Chloroplast transformation in microalgae offers great promise for the production of proteins of pharmaceutical interest or for the development of novel biofuels in the chloroplast and improve transgene expression. Introduction Microalgae are attracting increasing interest

  1. Dynamics of gene expression and signal transduction in single cells

    E-Print Network [OSTI]

    Yang, Qiong, Ph. D. Massachusetts Institute of Technology

    2009-01-01T23:59:59.000Z

    Each individual cell is a highly dynamic and complex system. Characterizing dynamics of gene expression and signal transduction is essential to understand what underlie the behavior of the cell and has stimulated much ...

  2. Exploiting quaternions to support expressive interactive character motion

    E-Print Network [OSTI]

    Johnson, Michael Patrick, 1971-

    2003-01-01T23:59:59.000Z

    A real-time motion engine for interactive synthetic characters, either virtual or physical, needs to allow expressivity and interactivity of motion in order to maintain the illusion of life. Canned animation examples from ...

  3. Proving Termination of Normalization Functions for Conditional Expressions

    E-Print Network [OSTI]

    Haddadi, Hamed

    Boyer and Moore have discussed a recursive function that puts con- ditional expressions software correctness) Keywords: Boyer/Moore Theorem Prover, LCF, total correctness, well 12 1 #12; 1 A normalization function Boyer

  4. Automatic 3D facial expression analysis in videos

    E-Print Network [OSTI]

    Chang, Y; Vieira, M; Turk, M; Velho, L

    2005-01-01T23:59:59.000Z

    with the background in videos [26] are important topics forSystem for Real-Time 3D Video. IEEE Int. Workshop onExpression Analysis in Videos Ya Chang 1 , Marcelo Vieira

  5. antibody expressing pea: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    can induce gene expression changes 10 Cooking and Using Dried Beans and Peas Renewable Energy Websites Summary: beans or peas with fresh water. Add 1 tablespoon of oil or other...

  6. Facing fear: Expression of fear facilitates processing of emotional information

    E-Print Network [OSTI]

    Schnall, Simone; Laird, James D.

    2007-01-01T23:59:59.000Z

    and frowns of politicians (Ottati et al., 1997) and colors perceived as happy and sad (Soldat & Sinclair, 2001) can have the same effects as moods. Like affective feelings, embodied expressions are repre- sentations of value, that is, of goodness... effects of facial expressions and postures on emotional experience and memory. Cognition and Emotion, 17, 787-797. Soldat, A. S., & Sinclair, R. C. (2001). Colors, smiles, and frowns: External affective cues can directly affect responses...

  7. The Role of Multiple Transcription Factors In Archaeal Gene Expression

    SciTech Connect (OSTI)

    Charles J. Daniels

    2008-09-23T23:59:59.000Z

    Since the inception of this research program, the project has focused on two central questions: What is the relationship between the 'eukaryal-like' transcription machinery of archaeal cells and its counterparts in eukaryal cells? And, how does the archaeal cell control gene expression using its mosaic of eukaryal core transcription machinery and its bacterial-like transcription regulatory proteins? During the grant period we have addressed these questions using a variety of in vivo approaches and have sought to specifically define the roles of the multiple TATA binding protein (TBP) and TFIIB-like (TFB) proteins in controlling gene expression in Haloferax volcanii. H. volcanii was initially chosen as a model for the Archaea based on the availability of suitable genetic tools; however, later studies showed that all haloarchaea possessed multiple tbp and tfb genes, which led to the proposal that multiple TBP and TFB proteins may function in a manner similar to alternative sigma factors in bacterial cells. In vivo transcription and promoter analysis established a clear relationship between the promoter requirements of haloarchaeal genes and those of the eukaryal RNA polymerase II promoter. Studies on heat shock gene promoters, and the demonstration that specific tfb genes were induced by heat shock, provided the first indication that TFB proteins may direct expression of specific gene families. The construction of strains lacking tbp or tfb genes, coupled with the finding that many of these genes are differentially expressed under varying growth conditions, provided further support for this model. Genetic tools were also developed that led to the construction of insertion and deletion mutants, and a novel gene expression scheme was designed that allowed the controlled expression of these genes in vivo. More recent studies have used a whole genome array to examine the expression of these genes and we have established a linkage between the expression of specific tfb genes and the regulation of nitrogen metabolism and other global cellular responses.

  8. Edinburgh Research Explorer High-resolution gene expression profiling for simultaneous

    E-Print Network [OSTI]

    Millar, Andrew J.

    Edinburgh Research Explorer High-resolution gene expression profiling for simultaneous kinetic, UH 2008, 'High-resolution gene expression profiling for simultaneous kinetic parameter analysis date: 16. Jun. 2014 #12;METHOD High-resolution gene expression profiling for simultaneous kinetic

  9. Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs.

    E-Print Network [OSTI]

    Yan, M; Wen, J; Liang, M; Lu, Y; Kamata, M; Chen, IS

    2015-01-01T23:59:59.000Z

    of Gene Expression by Polymer Nanocapsule S5 Fig. Stabilityof Gene Expression by Polymer Nanocapsule Rao D, Vorhies J,of Gene Expression by Polymer Nanocapsule Delivery of DNA

  10. Using Expressive and Flexible Action Representations to Reason about Capabilties for Intelligent Agent Cooperation 

    E-Print Network [OSTI]

    Wickler, Gerhard

    expressiveness and its flexibility. By expressiveness we mean the ability to express more than is possible in other representations. By flexibility we mean the possibility to delay decisions regarding the compromises that have to be made to knowledge...

  11. HIF-1 expression is associated with CCL2 chemokine expression in airway inflammatory cells: implications in allergic airway inflammation

    E-Print Network [OSTI]

    2012-01-01T23:59:59.000Z

    and CCL2 expression in the lung after intratracheal exposureand CCL2 were decreased in the lungs of mice that have beenin part, by HIF-1 in the lung. These findings also

  12. Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph

    SciTech Connect (OSTI)

    Daniel J Arp

    2005-06-15T23:59:59.000Z

    Nitrosomonas europaea is an aerobic lithoautotrophic bacterium that uses ammonia (NH3) as its energy source. As a nitrifier, it is an important participant in the nitrogen cycle, which can also influence the carbon cycle. The focus of this work was to explore the genetic structure and mechanisms underlying the lithoautotrophic growth style of N. europaea. Whole genome gene expression. The gene expression profile of cells in exponential growth and during starvation was analyzed using microarrays. During growth, 98% of the genes increased in expression at least two fold compared to starvation conditions. In growing cells, approximately 30% of the genes were expressed eight fold higher, Approximately 10% were expressed more than 15 fold higher. Approximately 3% (91 genes) were expressed to more than 20 fold of their levels in starved cells. Carbon fixation gene expression. N. europaea fixes carbon via the Calvin-Benson-Bassham (CBB) cycle via a type I ribulose bisphosphate carboxylase/oxygenase (RubisCO). This study showed that transcription of cbb genes was up-regulated when the carbon source was limited, while amo, hao and other energy harvesting related genes were down-regulated. Iron related gene expression. Because N. europaea has a relatively high content of hemes, sufficient Fe must be available in the medium for it to grow. The genome revealed that approximately 5% of the coding genes in N. europaea are dedicated to Fe transport and assimilation. Nonetheless, with the exception of citrate biosynthesis genes, N. europaea lacks genes for siderophore production. The Fe requirements for growth and the expression of the putative membrane siderophore receptors were determined. The N. europaea genome has over 100 putative genes ({approx}5% of the coding genes) related to Fe uptake and its siderophore receptors could be grouped phylogenetically in four clusters. Fe related genes, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin, and diffusion protein OmpC were expressed to higher levels under Fe limitation. N. europaea has a high Fe requirement and under Fe limiting conditions (0.2 ?M), is capable to assimilate up to 70% of the available Fe without the ability to produce siderophores.

  13. Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph

    SciTech Connect (OSTI)

    Daniel J. Arp

    2005-05-25T23:59:59.000Z

    Nitrosomonas europaea is an aerobic lithoautotrophic bacterium that uses ammonia (NH3) as its energy source. As a nitrifier, it is an important participant in the nitrogen cycle, which can also influence the carbon cycle. The focus of this work was to explore the genetic structure and mechanisms underlying the lithoautotrophic growth style of N. europaea. Whole genome gene expression: The gene expression profile of cells in exponential growth and during starvation was analyzed using microarrays. During growth, 98% of the genes increased in expression at least two fold compared to starvation conditions. In growing cells, approximately 30% of the genes were expressed eight fold higher, Approximately 10% were expressed more than 15 fold higher. Approximately 3% (91 genes) were expressed to more than 20 fold of their levels in starved cells. Carbon fixation gene expression: N. europaea fixes carbon via the Calvin-Benson-Bassham (CBB) cycle via a type I ribulose bisphosphate carboxylase/oxygenase (RubisCO). This study showed that transcription of cbb genes was up-regulated when the carbon source was limited, while amo, hao and other energy harvesting related genes were down-regulated. Iron related gene expression: Because N. europaea has a relatively high content of hemes, sufficient Fe must be available in the medium for it to grow. The genome revealed that approximately 5% of the coding genes in N. europaea are dedicated to Fe transport and assimilation. Nonetheless, with the exception of citrate biosynthesis genes, N. europaea lacks genes for siderophore production. The Fe requirements for growth and the expression of the putative membrane siderophore receptors were determined. The N. europaea genome has over 100 putative genes ({approx}5% of the coding genes) related to Fe uptake and its siderophore receptors could be grouped phylogenetically in four clusters. Fe related genes, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin, and diffusion protein OmpC were expressed to higher levels under Fe limitation. N. europaea has a high Fe requirement and under Fe limiting conditions (0.2 {micro}M), is capable to assimilate up to 70% of the available Fe without the ability to produce siderophores.

  14. Expression profile of host restriction factors in HIV-1 elite controllers

    E-Print Network [OSTI]

    2013-01-01T23:59:59.000Z

    et al. : Expression profile of host restriction factors inOpen Access Expression profile of host restriction factorsthat a transcriptional profile signature of CD4+ T cells is

  15. Gene co-expression network analysis in Rhodobacter capsulatus and application to comparative expression analysis of Rhodobacter sphaeroides

    SciTech Connect (OSTI)

    Pena-Castillo, Lourdes; Mercer, Ryan; Gurinovich, Anastasia; Callister, Stephen J.; Wright, Aaron T.; Westbye, Alexander; Beatty, J. T.; Lang, Andrew S.

    2014-08-28T23:59:59.000Z

    The genus Rhodobacter contains purple nonsulfur bacteria found mostly in freshwater environments. Representative strains of two Rhodobacter species, R. capsulatus and R. sphaeroides, have had their genomes fully sequenced and both have been the subject of transcriptional profiling studies. Gene co-expression networks can be used to identify modules of genes with similar expression profiles. Functional analysis of gene modules can then associate co-expressed genes with biological pathways, and network statistics can determine the degree of module preservation in related networks. In this paper, we constructed an R. capsulatus gene co-expression network, performed functional analysis of identified gene modules, and investigated preservation of these modules in R. capsulatus proteomics data and in R. sphaeroides transcriptomics data. Results: The analysis identified 40 gene co-expression modules in R. capsulatus. Investigation of the module gene contents and expression profiles revealed patterns that were validated based on previous studies supporting the biological relevance of these modules. We identified two R. capsulatus gene modules preserved in the protein abundance data. We also identified several gene modules preserved between both Rhodobacter species, which indicate that these cellular processes are conserved between the species and are candidates for functional information transfer between species. Many gene modules were non-preserved, providing insight into processes that differentiate the two species. In addition, using Local Network Similarity (LNS), a recently proposed metric for expression divergence, we assessed the expression conservation of between-species pairs of orthologs, and within-species gene-protein expression profiles. Conclusions: Our analyses provide new sources of information for functional annotation in R. capsulatus because uncharacterized genes in modules are now connected with groups of genes that constitute a joint functional annotation. We identified R. capsulatus modules enriched with genes for ribosomal proteins, porphyrin and bacteriochlorophyll anabolism, and biosynthesis of secondary metabolites to be preserved in R. sphaeroides whereas modules related to RcGTA production and signalling showed lack of preservation in R. sphaeroides. In addition, we demonstrated that network statistics may also be applied within-species to identify congruence between mRNA expression and protein abundance data for which simple correlation measurements have previously had mixed results.

  16. Recombinant cells that highly express chromosomally-integrated heterologous genes

    DOE Patents [OSTI]

    Ingram, Lonnie O. (Gainesville, FL); Ohta, Kazuyoshi (Gainesville, FL); Wood, Brent E. (Gainesville, FL)

    2000-08-22T23:59:59.000Z

    Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

  17. Recombinant cells that highly express chromosomally-integrated heterologous genes

    DOE Patents [OSTI]

    Ingram, L.O.; Ohta, Kazuyoshi; Wood, B.E.

    1998-10-13T23:59:59.000Z

    Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol. 13 figs.

  18. Recombinant cells that highly express chromosomally-integrated heterologous genes

    DOE Patents [OSTI]

    Ingram, Lonnie O. (Gainesville, FL); Ohta, Kazuyoshi (Gainesville, FL); Wood, Brent E. (Gainesville, FL)

    1998-01-01T23:59:59.000Z

    Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

  19. p27{sup Kip1} inhibits tissue factor expression

    SciTech Connect (OSTI)

    Breitenstein, Alexander, E-mail: alexander.breitenstein@usz.ch [Cardiology, University Heart Center, University Hospital Zurich (Switzerland) [Cardiology, University Heart Center, University Hospital Zurich (Switzerland); Cardiovascular Research, Physiology Institute, University of Zurich (Switzerland); Center for Integrative Human Physiology (ZHIP), University of Zurich (Switzerland); Akhmedov, Alexander; Camici, Giovanni G.; Lüscher, Thomas F.; Tanner, Felix C. [Cardiology, University Heart Center, University Hospital Zurich (Switzerland) [Cardiology, University Heart Center, University Hospital Zurich (Switzerland); Cardiovascular Research, Physiology Institute, University of Zurich (Switzerland); Center for Integrative Human Physiology (ZHIP), University of Zurich (Switzerland)

    2013-10-04T23:59:59.000Z

    Highlights: •p27{sup Kip1}regulates the expression of tissue factor at the transcriptional level. •This inhibitory effect of p27{sup Kip1} is independently of its cell regulatory action. •The current study provides new insights into a pleiotrophic function of p27{sup Kip1}. -- Abstract: Background: The cyclin-dependent kinase inhibitor (CDKI) p27{sup Kip1} regulates cell proliferation and thus inhibits atherosclerosis and vascular remodeling. Expression of tissue factor (TF), the key initator of the coagulation cascade, is associated with atherosclerosis. Yet, it has not been studied whether p27{sup Kip1} influences the expression of TF. Methods and results: p27{sup Kip1} overexpression in human aortic endothelial cells was achieved by adenoviral transfection. Cells were rendered quiescent for 24 h in 0.5% fetal-calf serum. After stimulation with TNF-? (5 ng/ml), TF protein expression and activity was significantly reduced (n = 4; P < 0.001) in cells transfected with p27{sup Kip1}. In line with this, p27{sup Kip1} overexpression reduced cytokine-induced TF mRNA expression (n = 4; P < 0.01) and TF promotor activity (n = 4; P < 0.05). In contrast, activation of the MAP kinases p38, ERK and JNK was not affected by p27{sup Kip1} overexpression. Conclusion: This in vitro study suggests that p27{sup Kip1} inhibits TF expression at the transcriptional level. These data indicate an interaction between p27{sup Kip1} and TF in important pathological alterations such as atherosclerosis and vascular remodeling.

  20. Urethane anesthesia blocks the development and expression of kindled seizures

    SciTech Connect (OSTI)

    Cain, D.P.; Raithby, A.; Corcoran, M.E.

    1989-01-01T23:59:59.000Z

    The effect of anesthetic and subanesthetic doses of urethane on the development of amygdala kindled seizures and on the expression of previously kindled seizures was studied in hooded rats. An anesthetic dose of urethane almost completely eliminated evoked after discharge and completely eliminated convulsive behavior in both groups. It also eliminated the seizure response to pentylenetetrazol. Subanesthetic doses of urethane strongly attenuated the expression of previously kindled seizures. These results suggest that urethane may not be an appropriate anesthetic for the study of epileptiform phenomena.

  1. Defining, expressing, and using context in a simulation environment

    SciTech Connect (OSTI)

    Hummel, J. R.; Christiansen, J. H.

    2000-02-16T23:59:59.000Z

    Reuse and interoperability are two keywords in the mantra of the modeling and simulation community. In order to achieve these goals, one must be able to capture, express, and manage the context of individual entities, models, and applications. Capturing the context requires having a thorough understanding of what the entity, model, or application was intended to do and is able to do. While many aspects of context are not easily expressible in a format or language that could be understood and managed in a simulation environment, there are some aspects that can be and the authors discuss how these aspects can be represented in a generalized object-oriented framework.

  2. Oscillations in probability distributions for stochastic gene expression

    SciTech Connect (OSTI)

    Petrosyan, K. G., E-mail: pkaren@phys.sinica.edu.tw; Hu, Chin-Kun, E-mail: huck@phys.sinica.edu.tw [Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan (China)

    2014-05-28T23:59:59.000Z

    The phenomenon of oscillations in probability distribution functions of number of components is found for a model of stochastic gene expression. It takes place in cases of low levels of molecules or strong intracellular noise. The oscillations distinguish between more probable even and less probable odd number of particles. The even-odd symmetry restores as the number of molecules increases with the probability distribution function tending to Poisson distribution. We discuss the possibility of observation of the phenomenon in gene, protein, and mRNA expression experiments.

  3. Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast

    DOE Patents [OSTI]

    Mayfield, Stephen P. (Cardiff, CA)

    2010-03-16T23:59:59.000Z

    Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.

  4. Facial Expression Recognition Based on The Belief Theory: Comparison With Different Classifiers

    E-Print Network [OSTI]

    Dupont, Stéphane

    Copernic, B-7000, Mons, Belgium Abstract. This paper presents a system for classifying facial expressions

  5. Increased fat oxidation in 3T3-L1 adipocytes through forced expression of UCP 1

    E-Print Network [OSTI]

    Palani, Santhosh

    2006-10-30T23:59:59.000Z

    ...................... 27 Regulation of EGFP Expression with DOX....................................... 29 Adipocytes Metabolic Assays ............................................................. 31 Functional Evaluation of UCP1 Expressing Adipocytes... of preadipocytes and mature adipocytes; (B) Fluorescence images of preadipocytes and mature adipocytes. 29 Regulation of EGFP Expression with DOX- Expression of EGFP was modulated using the chemical inducer doxycycline. Addition...

  6. RT-PCR cloning of expressed VSGs Harvesting cells

    E-Print Network [OSTI]

    Cross, George

    RT-PCR cloning of expressed VSGs Harvesting cells Starting material: 5­10x107 cells (BF) Centrifuge for at least 1 month). RNA extraction STAT-60 according to manufacturer's instructions. Resuspend RNA in 50 µl of DEPC-treated water. Measuring the A260 will suggest a yield of ~ 100 µg RNA from 108 cells

  7. Inflammatory Gene Expression in Goats in Response to Transport

    E-Print Network [OSTI]

    Carter, Mark

    2012-10-19T23:59:59.000Z

    was to determine whether expression of immune-related genes changes in goats that are exposed to transport stress. In this study, 15 Spanish-Boer goats ranging from 3 to 4 yrs of age were transported for 12 h. Goats were divided into 5 groups of 3 and placed in 1...

  8. Expressing Default Abduction Problems as Quantified Boolean Formulas

    E-Print Network [OSTI]

    Tompits, Hans

    1 Expressing Default Abduction Problems as Quantified Boolean Formulas Hans Tompits Institut f@kr.tuwien.ac.at Abduction is the process of finding explanations for observed phenomena in accord to known laws about, we deal with a model for abduction in which the domain knowledge is represented in terms of a default

  9. EXPRESSING COLLABORATION AND COMPETITION AMONG ABDUCTIVE LOGIC AGENTS

    E-Print Network [OSTI]

    Torroni, Paolo

    EXPRESSING COLLABORATION AND COMPETITION AMONG ABDUCTIVE LOGIC AGENTS Anna Ciampolini, Evelina a language for coordinating several logic­based agents capable of abductive reasoning. The system to guess the causes of some symptoms. In his respect, some form of open or abductive reasoning has

  10. Expressive Completeness of an Event-Pattern Reactive Programming Language

    E-Print Network [OSTI]

    Sánchez, César

    Control Air Frame (a) With no event correlation. Navigation Display Navigation Steering Tactical Steering to temporal patterns. Our general model of event-pattern re- actions can express every "reasonable" model or an informal description of #12;2 Navigation Display Navigation Steering Tactical Steering Middleware GPS Pilot

  11. Expressive Completeness of an EventPattern Reactive Programming Language

    E-Print Network [OSTI]

    Sánchez, César

    Pilot Control Air Frame (a) With no event correlation. Navigation Display Navigation Steering Tactical Steering Middleware GPS Pilot Control Air Frame EC EC EC EC (b) With event correlation. Fig. 1: A simple to temporal patterns. Our general model of event­pattern re­ actions can express every ``reasonable'' model

  12. Prototypes with Multiple Dispatch: An Expressive and Dynamic Object Model

    E-Print Network [OSTI]

    Aldrich, Jonathan

    - namically typed programming language Slate. 1 Overview We begin the paper by describing a motivating example operational semantics. Section 5 demonstrates the expressiveness of PMD through the standard library of Slate for implementing dispatch in Slate. Section 7 describes related work, and section 8 concludes. #12;2 Motivating

  13. Symbolic Discriminant Analysis for Mining Gene Expression Patterns Jason Moore

    E-Print Network [OSTI]

    Fernandez, Thomas

    multivariate statistical approach for classification of observations into groups because the theory is wellSymbolic Discriminant Analysis for Mining Gene Expression Patterns Jason Moore Vanderbilt: Leukemia Jason H. Moore, Joel S. Parker, Lance W. Hahn Linear discriminant analysis is a popular

  14. NANO EXPRESS Fabrication of Large Area Periodic Nanostructures Using

    E-Print Network [OSTI]

    Mohseni, Hooman

    , such as photonic band-gap materials, high dense data storage, and photonic devices. We have developed a maskless areas, such as photonic band-gap materials [1], high dense data storage [2], and photonic devices [3NANO EXPRESS Fabrication of Large Area Periodic Nanostructures Using Nanosphere Photolithography

  15. NANO EXPRESS Open Access Multiscale investigation of graphene layers on

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    NANO EXPRESS Open Access Multiscale investigation of graphene layers on 6H-SiC(000-1) Antoine , Cecile Naud2 , Jean-Yves Veuillen2* Abstract In this article, a multiscale investigation of few graphene, the authors show that the UHV growth yields few layer graphene (FLG) with an average thickness given by Auger

  16. Arabinogalactan-proteins: structure, expression and function A. M. Showalter

    E-Print Network [OSTI]

    Showalter, Allan M.

    Arabinogalactan-proteins: structure, expression and function A. M. Showalter Department, it does clearly droxyproline poor, lightly glycosylated and largely unre- active with Yariv reagent with a synthetic chemical reagent, a phenylazoglycoside dye called Yariv reagent (fig. 1) [1, 2]. Such generalities

  17. ORIGINAL PAPER Expression of genes involved in energy mobilization

    E-Print Network [OSTI]

    Lee Jr., Richard E.

    ORIGINAL PAPER Expression of genes involved in energy mobilization and osmoprotectant synthesis, mobilizing carbohydrate energy reserves to promote synthesis of low-molecular-mass osmoprotectants Department of Entomology, Ohio State University, Columbus, OH 43210, USA e-mail: teets.23@osu.edu Y

  18. Noise-based switches and amplifiers for gene expression

    E-Print Network [OSTI]

    Hasty, Jeff

    controlled at the level of gene transcription. Such genetic regulation usually consists of interacting and manipulation of such networks lies within the constraints of current technology. Here we develop a model can be used to regulate expression. In the additive case, we demonstrate the utility of such control

  19. NEWS AND VIEWS Modeling gene expression control using Omes Law

    E-Print Network [OSTI]

    Nguyen, Dat H.

    NEWS AND VIEWS Modeling gene expression control using Omes Law Harmen J Bussemaker Department class of methods that fit simple mathematical models of transcription regulation to DNA microarray data factors (TFs) to specific sites in the genome is a crucial step in the molecular process controlling gene

  20. Similarities and Differences in Genome-Wide Expression Data

    E-Print Network [OSTI]

    Barkai, Naama

    Ihmels, Naama Barkai* Departments of Molecular Genetics and Physics of Complex Systems, Weizmann profiles are similar, we find that for all organisms the connectivity distribution follows a power. We reveal the modular structure by decomposing each set of expression data into coexpressed modules

  1. Revising Regulatory Networks: From Expression Data to Linear Causal Models

    E-Print Network [OSTI]

    Langley, Pat

    network structure. However, this ignores much ex- isting knowledge because for a given organism and system under study, a biologist may already have a partial model of gene regulation. We propose a method, with expression data. We demonstrate our approach by revising a model of photosynthesis regulation proposed

  2. Phase-plane visualizations of gestural structure in expressive timing

    E-Print Network [OSTI]

    Widmer, Gerhard

    , and describe the construction of the phase-plane representa- tion from the time series using a functional has been one of the major topics in music perfor- mance research. As an expressive parameter, timing is used to clarify the musical structure of the piece (Clarke, 1988), among other things. Background

  3. Expressing and Exploiting Concurrency in Networked Applications with Aspen

    E-Print Network [OSTI]

    Expressing and Exploiting Concurrency in Networked Applications with Aspen Gautam Upadhyaya, Vijay,vpai,smidkiff}@purdue.edu Abstract This paper presents Aspen, a high-level programming language that targets both high-productivity programming and runtime sup- port for managing resources needed by a computation. Programs in Aspen

  4. Monday, March 13, 2006 MARS EXPRESS: PROBING THE DEPTHS

    E-Print Network [OSTI]

    Rathbun, Julie A.

    . Plaut J. J. Observations of the "Stealth" Radar Feature in the Mars Express MARSIS Investigation [#1946] We will present the latest observations of the "Stealth" radar feature by the MARSIS radar. 9:30 a and Plains materials in Gusev, complementing in situ Spirit observations. 10:15 a.m. Bibring J.-P. * Langevin

  5. Up-Regulated Dicer Expression in Patients with Cutaneous Melanoma

    E-Print Network [OSTI]

    Oliver, Douglas L.

    Up-Regulated Dicer Expression in Patients with Cutaneous Melanoma Zhihai Ma1 , Helen Swede2 , David clinically annotated controls and skin tumors consisting of melanocytic nevi (n = 71), a variety of melanomas Dicer in 81% of cutaneous, 80% of acrolentiginous and 96% of metastatic melanoma specimens compared

  6. Expressive Visualization and Rapid Interpretation of Seismic Volumes

    E-Print Network [OSTI]

    Expressive Visualization and Rapid Interpretation of Seismic Volumes Daniel Patel Thesis, Christopher Giertsen, John Thurmond, Eduard Gr¨oller The Seismic Analyzer: Interpreting and Illustrating 2D of Seismic Data Published in: Vision, Modelling and Visualization (VMV) 2007 Authors: Daniel Patel

  7. MICROFLUIDIC DEVICES FOR GENENTIC ANAYLSIS AND GENE EXPRESSION STUDIES

    E-Print Network [OSTI]

    Quake, Stephen R.

    MICROFLUIDIC DEVICES FOR GENENTIC ANAYLSIS AND GENE EXPRESSION STUDIES Thesis by Jian Liu field of microfluidics. His advice has helped me out many times when I was struggling in my research, Ziyang Ma, Emil Kartalov, and many other colleagues. I thank Dr. Jessica Melin and the microfluidic

  8. RESEARCH ARTICLE Open Access Detecting differential allelic expression using

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    gene. We aimed to assess whether CHEK2 was subject to DAE in lymphoblastoid cell lines (LCLs) from high for DAE assessment. Results: We observed allelic expression imbalance in 4 of the 41 LCLs examined. All in the transcriptional regulatory milieu of freely proliferating LCLs. Conclusions: Our results support that HRM

  9. Indexing and Querying XML Data for Regular Path Expressions

    E-Print Network [OSTI]

    Moon, Bongki

    by generating interactive site maps [19]. To retrieve XML and semi-structured data, several query languages have of XML data sources from docu- ments to databases and object repositories. The common featuresIndexing and Querying XML Data for Regular Path Expressions £ Quanzhong Li Bongki Moon Dept

  10. Plasticity of opsin gene expression in cichlids from Lake Malawi

    E-Print Network [OSTI]

    Carleton, Karen L.

    Plasticity of opsin gene expression in cichlids from Lake Malawi CHRISTOPHER M. HOFMANN, KELLY E. O plasticity has important evolutionary implications. In this study, we examined retinal plasticity in five found that the magnitude of plasticity varied across species. These findings have important implications

  11. On some arithmetic properties of polynomial expressions involving Stirling numbers

    E-Print Network [OSTI]

    Klazar, Martin

    On some arithmetic properties of polynomial expressions involving Stirling numbers of the second by the grant SEP-CONACYT 37259-E. 1 #12;Abstract Let S(n, k) be the classical Stirling numbers of the second. We give similar but more particular results on the more general Stirling-like numbers T(n, k). 2 #12

  12. Genome Organization and Gene Expression Shape the Transposable Element Distribution

    E-Print Network [OSTI]

    Alvarez, Nadir

    Genome Organization and Gene Expression Shape the Transposable Element Distribution The distribution of transposable elements (TEs) in a genome reflects a balance between insertion rate and selection shaping the organization of genomes. Past research has shown that TEs tend to accumulate in genomic

  13. EPAC expression and function in cardiac fibroblasts and myofibroblasts

    SciTech Connect (OSTI)

    Olmedo, Ivonne; Muñoz, Claudia; Guzmán, Nancy; Catalán, Mabel; Vivar, Raúl; Ayala, Pedro; Humeres, Claudio; Aránguiz, Pablo [Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile (Chile); García, Lorena [Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile (Chile); Velarde, Victoria [Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile (Chile); Díaz-Araya, Guillermo, E-mail: gadiaz@ciq.uchile.cl [Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile (Chile)

    2013-10-15T23:59:59.000Z

    In the heart, cardiac fibroblasts (CF) and cardiac myofibroblasts (CMF) are the main cells responsible for wound healing after cardiac insult. Exchange protein activated by cAMP (EPAC) is a downstream effector of cAMP, and it has been not completely studied on CF. Moreover, in CMF, which are the main cells responsible for cardiac healing, EPAC expression and function are unknown. We evaluated in both CF and CMF the effect of transforming growth factor ?1 (TGF-?1) on EPAC-1 expression. We also studied the EPAC involvement on collagen synthesis, adhesion, migration and collagen gel contraction. Method: Rat neonatal CF and CMF were treated with TGF-?1 at different times and concentrations. EPAC-1 protein levels and Rap1 activation were measured by western blot and pull down assay respectively. EPAC cellular functions were determined by adhesion, migration and collagen gel contraction assay; and collagen expression was determined by western blot. Results: TGF-?1 through Smad and JNK significantly reduced EPAC-1 expression in CF, while in CMF this cytokine increased EPAC-1 expression through ERK1/2, JNK, p38, AKT and Smad3. EPAC activation was able to induce higher Rap1-GTP levels in CMF than in CF. EPAC and PKA, both cAMP effectors, promoted CF and CMF adhesion on fibronectin, as well as CF migration; however, this effect was not observed in CMF. EPAC but not PKA activation mediated collagen gel contraction in CF, while in CMF both PKA and EPAC mediated collagen gel contraction. Finally, the EPAC and PKA activation reduced collagen synthesis in CF and CMF. Conclusion: TGF-?1 differentially regulates the expression of EPAC in CF and CMF; and EPAC regulates differentially CF and CMF functions associated with cardiac remodeling. - Highlights: • TGF-?1 regulates EPAC-1 expression in cardiac fibroblast and myofibroblast. • Rap-1GTP levels are higher in cardiac myofibroblast than fibroblast. • EPAC-1 controls adhesion, migration and collagen synthesis in cardiac fibroblast. • PKA regulates collagen gel contraction in cardiac myofibroblast.

  14. Differential expression of murine adult hemoglobins in early ontogeny

    SciTech Connect (OSTI)

    Wawrzyniak, C.J.; Lewis, S.E.; Popp, R.A.

    1985-01-01T23:59:59.000Z

    A hemoglobin mutation is described that permits study of the expression of the two adult ..beta..-globin genes throughout fetal and postnatal development. Mice with a mutation at the Hbb/sup s/, ..beta..-globin locus, were used to study the relative levels of ..beta..-s2major and ..beta..-sminor globins specified by the mutant Hbb/sup s2/ haplotype during development. At 11.5 days of gestation ..beta..-sminor comprised over 80% and ..beta..-s2major under 20% of the adult beta-globin. The relative level of ..beta..-sminor decreased through fetal development; at birth ..beta..-sminor represented 33.7% of the ..beta..-globin. The adult values of 71.0% ..beta..-s2major and 29.0% ..beta..-sminor globin are expressed in mice six days after birth. Because the two ..beta..-globin genes are expressed in mice of the Hbb/sup 2s/ haplotype, both the ..beta..-smajor and ..beta..-sminor genes must be expressed in mice of the Hbb/sup s/ haplotype. Expression of the ..beta..-sminor gene is elevated to 35.6% in Hbb/sup s2/ mice that have been bled repeatedly. Thus, the 5' ..beta..-s2major and 3' ..beta..-sminor genes of the Hbb/sup s2/ haplotype and, presumably the 5' ..beta..-smajor and 3' ..beta..-sminor genes of the Hbb/sup s/ haplotype, are regulated independently and are homologous to the 5' ..beta..-dmajor and 3' ..beta..-dminor genes of the Hbb/sup d/ haplotype. Mice of the Hbb/sup s2/ haplotype are better than mice of the Hbb/sup d/ haplotytpe for studying the mechanisms of hemoglobin switching because the Hbb/sup s2/ each of the three embryonic and two adult hemoglobins can be separated by electrophoresis. 17 refs., 3 figs.

  15. Simple and Compact Expressions for Neutrino Oscillation Probabilities in Matter

    E-Print Network [OSTI]

    Minakata, Hisakazu

    2015-01-01T23:59:59.000Z

    We reformulate perturbation theory for neutrino oscillations in matter with an expansion parameter related to the ratio of the solar to the atmospheric Delta m^2 scales. Unlike previous works, we use a renormalized basis in which certain first-order effects are taken into account in the zeroth-order Hamiltonian. Using this perturbation theory we derive extremely compact expressions for the neutrino oscillation probabilities in matter. We find, for example, that the $\

  16. Heterocyst Morphogenesis and Gene Expression in Anabaena sp. PCC 7120

    E-Print Network [OSTI]

    Mella Herrera, Rodrigo Andres

    2010-10-12T23:59:59.000Z

    RT-PCR) ....................... 104 Results ..................................................................................................... 105 NtcA interacts with the promoter region of sigE in vitro. .................... 105 nifH expression is delayed and reduced... in controlling heterocyst development (96). NtcA, a transcriptional regulator belonging to the CRP (cyclic AMP receptor protein) family of proteins, senses 2-oxoglutarate levels. The protein is conserved in all cyanobacteria and regulates a number of genes...

  17. MicroRNA expression in canine mammary cancer

    E-Print Network [OSTI]

    Boggs, Rene' Michelle

    2008-10-10T23:59:59.000Z

    to act as both tumor suppressors and oncogenes in several different cancers, expression patterns of ten miRNAs (miR-15a, miR-16, miR-17-5p, miR-21, miR-29b, miR-125b, miR-145, miR-155, miR-181b, let-7f) known to be associated with human breast cancer were...

  18. Bitumen fume-induced gene expression profile in rat lung

    SciTech Connect (OSTI)

    Gate, Laurent [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France)]. E-mail: laurent.gate@inrs.fr; Langlais, Cristina [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France); Micillino, Jean-Claude [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France); Nunge, Herve [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France); Bottin, Marie-Claire [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France); Wrobel, Richard [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France); Binet, Stephane [Institut National de Recherche et Securite, Avenue de Bourgogne, BP 27, 54501 Vandoeuvre Cedex (France)

    2006-08-15T23:59:59.000Z

    Exposure to bitumen fumes during paving and roofing activities may represent an occupational health risk. To date, most of the studies performed on the biological effect of asphalt fumes have been done with regard to their content in carcinogenic polycyclic aromatic hydrocarbons (PAH). In order to gain an additional insight into the mechanisms of action of bitumen fumes, we studied their pulmonary effects in rodents following inhalation using the microarray technology. Fisher 344 rats were exposed for 5 days, 6 h/day to bitumen fumes generated at road paving temperature (170 {sup o}C) using a nose-only exposition device. With the intention of studying the early transcriptional events induced by asphalt fumes, lung tissues were collected immediately following exposure and gene expression profiles in control and exposed rats were determined by using oligonucleotide microarrays. Data analysis revealed that genes involved in lung inflammatory response as well as genes associated with PAH metabolization and detoxification were highly expressed in bitumen-exposed animals. In addition, the expression of genes related to elastase activity and its inhibition which are associated with emphysema was also modulated. More interestingly genes coding for monoamine oxidases A and B involved in the metabolism of neurotransmitters and xenobiotics were downregulated in exposed rats. Altogether, these data give additional information concerning the bitumen fumes biological effects and would allow to better review the health effects of occupational asphalt fumes exposure.

  19. 2001 Gordon Research Conference on Radiation Oncology. Final progress report [agenda and attendee list

    SciTech Connect (OSTI)

    Little, John B.

    2001-02-02T23:59:59.000Z

    The Gordon Research Conference on Radiation Oncology was held at the Holiday Inn, Ventura, California, from 1/28/01 through 2/2/01. The conference was well attended with 140 participants. The attendees represented the spectrum of endeavor in this field, coming from academia, industry, and government laboratories, and included U.S. and foreign scientists, senior researchers, young investigators, and students. Emphasis was placed on current unpublished research and discussion of the future target areas in this field. There was a conscious effort to stimulate lively discussion about the key issues in the field today. Time for formal presentations was limited; poster presentation time was scheduled, as well as ''free time'' to allow informal discussions. Sessions were held on the following topics: Recombinational processes in radiation response; Damage recognition pathways; Oxidative stress; Damage response pathways; Radiation-induced genomic instability; Mechanisms of mammalian cell death; New radiation models; Translational models; Bystander effects.

  20. Population genetic variation in gene expression is associated withphenotypic variation in Saccharomyces cerevisiae

    SciTech Connect (OSTI)

    Fay, Justin C.; McCullough, Heather L.; Sniegowski, Paul D.; Eisen, Michael B.

    2004-02-25T23:59:59.000Z

    The relationship between genetic variation in gene expression and phenotypic variation observable in nature is not well understood. Identifying how many phenotypes are associated with differences in gene expression and how many gene-expression differences are associated with a phenotype is important to understanding the molecular basis and evolution of complex traits. Results: We compared levels of gene expression among nine natural isolates of Saccharomyces cerevisiae grown either in the presence or absence of copper sulfate. Of the nine strains, two show a reduced growth rate and two others are rust colored in the presence of copper sulfate. We identified 633 genes that show significant differences in expression among strains. Of these genes,20 were correlated with resistance to copper sulfate and 24 were correlated with rust coloration. The function of these genes in combination with their expression pattern suggests the presence of both correlative and causative expression differences. But the majority of differentially expressed genes were not correlated with either phenotype and showed the same expression pattern both in the presence and absence of copper sulfate. To determine whether these expression differences may contribute to phenotypic variation under other environmental conditions, we examined one phenotype, freeze tolerance, predicted by the differential expression of the aquaporin gene AQY2. We found freeze tolerance is associated with the expression of AQY2. Conclusions: Gene expression differences provide substantial insight into the molecular basis of naturally occurring traits and can be used to predict environment dependent phenotypic variation.

  1. Imaging gene expression in real-time using aptamers

    SciTech Connect (OSTI)

    Shin, Il Chung [Ames Laboratory

    2012-11-02T23:59:59.000Z

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging microscopy). Real-time transcription was measured by FLIM-FRET, which was detected by the decrease in donor lifetime resulting from ligand binding to IMAGEtags that were newly synthesized from the activated GAL1 promoter. The FRET signal was specific for transcribed IMAGEtags.

  2. Imaging gene expression in real-time using aptamers

    SciTech Connect (OSTI)

    Shin, Il Chung

    2011-12-13T23:59:59.000Z

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging microscopy). Real-time transcription was measured by FLIM-FRET, which was detected by the decrease in donor lifetime resulting from ligand binding to IMAGEtags that were newly synthesized from the activated GAL1 promoter. The FRET signal was specific for transcribed IMAGEtags.

  3. Supplementary Figure 1: ChR2-EYFP expression after fear conditioning recapitulates endogenous c-fos expression.

    E-Print Network [OSTI]

    Schnitzer, Mark

    -fos expression. The c-fos-tTA mice were injected with AAV9-TRE-ChR2-EYFP targeting the DG and kept on Dox for a month prior to training. Then, they were taken off Dox for two days to open a window of activity­positive cells do not overlap. (a) DG from experimental mice kept off Dox for two days and then subjected to fear

  4. Transgenic mice expressing mutant Pinin exhibit muscular dystrophy, nebulin deficiency and elevated expression of slow-type muscle fiber genes

    SciTech Connect (OSTI)

    Wu, Hsu-Pin; Hsu, Shu-Yuan [Department of Anatomy, Chang Gung University Medical College, Taiwan (China)] [Department of Anatomy, Chang Gung University Medical College, Taiwan (China); Wu, Wen-Ai; Hu, Ji-Wei [Transgenic Mouse Core Laboratory, Chang Gung University, Taiwan (China)] [Transgenic Mouse Core Laboratory, Chang Gung University, Taiwan (China); Ouyang, Pin, E-mail: ouyang@mail.cgu.edu.tw [Department of Anatomy, Chang Gung University Medical College, Taiwan (China) [Department of Anatomy, Chang Gung University Medical College, Taiwan (China); Transgenic Mouse Core Laboratory, Chang Gung University, Taiwan (China); Molecular Medicine Research Center, Chang Gung University, Taiwan (China)

    2014-01-03T23:59:59.000Z

    Highlights: •Pnn CCD domain functions as a dominant negative mutant regulating Pnn expression and function. •Pnn CCD mutant Tg mice have a muscle wasting phenotype during development and show dystrophic histological features. •Pnn mutant muscles are susceptible to slow fiber type gene transition and NEB reduction. •The Tg mouse generated by overexpression of the Pnn CCD domain displays many characteristics resembling NEB{sup +/?} mice. -- Abstract: Pinin (Pnn) is a nuclear speckle-associated SR-like protein. The N-terminal region of the Pnn protein sequence is highly conserved from mammals to insects, but the C-terminal RS domain-containing region is absent in lower species. The N-terminal coiled-coil domain (CCD) is, therefore, of interest not only from a functional point of view, but also from an evolutionarily standpoint. To explore the biological role of the Pnn CCD in a physiological context, we generated transgenic mice overexpressing Pnn mutant in skeletal muscle. We found that overexpression of the CCD reduces endogenous Pnn expression in cultured cell lines as well as in transgenic skeletal muscle fibers. Pnn mutant mice exhibited reduced body mass and impaired muscle function during development. Mutant skeletal muscles show dystrophic histological features with muscle fibers heavily loaded with centrally located myonuclei. Expression profiling and pathway analysis identified over-representation of genes in gene categories associated with muscle contraction, specifically those related to slow type fiber. In addition nebulin (NEB) expression level is repressed in Pnn mutant skeletal muscle. We conclude that Pnn downregulation in skeletal muscle causes a muscular dystrophic phenotype associated with NEB deficiency and the CCD domain is incapable of replacing full length Pnn in terms of functional capacity.

  5. MODELING GENE EXPRESSION WITH DIFFERENTIAL Department of Genetics, Harvard Medical School

    E-Print Network [OSTI]

    Church, George M.

    MODELING GENE EXPRESSION WITH DIFFERENTIAL EQUATIONSa TING CHEN Department of Genetics, Harvard for gene expression and provide two methods to constructthe model from a set of temporaldata. We model both tran- scriptionand translationby kinetic equationswith feedbackloops from translation products

  6. Linking Stochastic Dynamics to Population Distribution: An Analytical Framework of Gene Expression

    E-Print Network [OSTI]

    Xie, Xiaoliang Sunney

    expression. (a) Kinetic schLinking Stochastic Dynamics to Population Distribution: An Analytical Framework of Gene Expression propagation in a simple genetic network. This model allows for the extraction of kinetic parameters of gene

  7. Mining large collections of gene expression data to elucidate transcriptional regulation of biological processes 

    E-Print Network [OSTI]

    Curry, Edward William James

    2011-06-27T23:59:59.000Z

    A vast amount of gene expression data is available to biological researchers. As of October 2010, the GEO database has 45,777 chips of publicly available gene expression pro ling data from the Affymetrix (HGU133v2) ...

  8. Using Plan Recognition for Interpreting Referring Expressions Dustin A. Smith and Henry Lieberman

    E-Print Network [OSTI]

    Using Plan Recognition for Interpreting Referring Expressions Dustin A. Smith and Henry Lieberman MIT Media Lab 20 Ames St. Cambridge, MA 02139 Abstract Referring expressions such as "a long meeting

  9. Area-Efficient Evaluation of Arithmetic Expressions Using Deeply Pipelined Floating-Point Cores1

    E-Print Network [OSTI]

    Prasanna, Viktor K.

    of such expressions must be evaluated in molecular dynamics simulations, for example. Such expressions usually have of Electrical Engineering University of Southern California Los Angeles, CA¢ rscrofan, lzhuo, prasanna£ @usc

  10. Methods and constructs for expression of foreign proteins in photosynthetic organisms

    DOE Patents [OSTI]

    Laible, Philip D. (Villa Park, IL); Hanson, Deborah K. (Downers Grove, IL)

    2002-01-01T23:59:59.000Z

    A method for expressing and purifying foreign proteins in photosynthetic organisms comprising the simultaneous expression of both the heterologous protein and a means for compartmentalizing or sequestering of the protein.

  11. The body parametric : abstraction of vocal and physical expression in performance

    E-Print Network [OSTI]

    Nattinger, Elena Jessop

    2014-01-01T23:59:59.000Z

    Performing artists have frequently used technology to sense and extend the body's natural expressivity via live control of multimedia. However, the sophistication, emotional content, and variety of expression possible ...

  12. Ambiguity and Constraint in Mathematical Expression Recognition Erik G. Miller and Paul A. Viola

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    Ambiguity and Constraint in Mathematical Expression Recognition Erik G. Miller and Paul A. Viola for the recognition of mathematical expressions. Several au- thors (Winkler, Fahrner, & Lang 1995; Hull 1996; Chou

  13. RESEARCH ARTICLE Open Access Bridging the gap between gene expression and

    E-Print Network [OSTI]

    , and metabolome data using kinetic models to create a mechanistic link between gene expression and metabolism expression, Kinetic models, Metabolic networks, S. cerevisiae, Transcriptomics, Fluxomics, MetabolomicsRESEARCH ARTICLE Open Access Bridging the gap between gene expression and metabolic phenotype via

  14. Effect of 1918 PB1-F2 Expression on Influenza A Virus Infection Kinetics

    E-Print Network [OSTI]

    Adler, Fred

    Effect of 1918 PB1-F2 Expression on Influenza A Virus Infection Kinetics Amber M. Smith1 al. (2011) Effect of 1918 PB1-F2 Expression on Influenza A Virus Infection Kinetics. PLoS Comput Biol influenza A virus PR8 or a genetically engineered virus that expresses the 1918 PB1-F2 protein on a PR8

  15. Modeling the segment polarity gene network First: System is biologically defined; known expression patterns

    E-Print Network [OSTI]

    Albert, Réka

    are assumed to be not expressed. · Generate a set of kinetic parameters from the biologically relevant rangeModeling the segment polarity gene network First: System is biologically defined; known expression Boolean model: mRNA and protein activity is switch-like Validation: reproduces known gene expression

  16. Expression of CX3CR1 chemokine receptors on neurons and their role in neuronal survival

    E-Print Network [OSTI]

    Meucci, Olimpia

    Expression of CX3CR1 chemokine receptors on neurons and their role in neuronal survival Olimpia expression of CX3CR1, the only known receptor for fractalkine, has been demonstrated exclusively on microglia, that hippocampal neurons also express CX3CR1. Receptor activation by soluble fractalkine induces activation

  17. A Theory for Comparing the Expressive Power of Access Control Mahesh V. Tripunitara Ninghui Li

    E-Print Network [OSTI]

    Li, Ninghui

    A Theory for Comparing the Expressive Power of Access Control Models Mahesh V. Tripunitara Ninghui,ninghui}@cerias.purdue.edu Abstract Comparing the expressive power of access control models is recognized as a fundamental problem it im- possible to put results and claims about the expressive power of access control models

  18. Dumbarton Express Line DB heading to Stanford Oval. AC Transit Line U on Palm Drive.

    E-Print Network [OSTI]

    Dumbarton Express Line DB heading to Stanford Oval. AC Transit Line U on Palm Drive. Photo: Karl Nielsen Photo: Dong Wang Schedules: AC Transit Line U Dumbarton Express Line DB Connections with BART Transit Line U Line U provides express service between Stanford and the Fremont BART station. Other East

  19. A Model of Gene Expression and Regulation in an Artificial Cellular Organism

    E-Print Network [OSTI]

    Fernandez, Thomas

    expression and regulation based on the operon model of Jacob and Monod. Operons are groups of genes regulated and complexity of gene expression in real cells and it should also bear close relationships to EC so that it can, Jacob and Monod developed the operon model of gene expression and regulation [1, 5, 7] to explain

  20. Differential regulation of RANTES and IL-8 expression in lung adenocarcinoma cells

    E-Print Network [OSTI]

    Boyer, Edmond

    1 Differential regulation of RANTES and IL-8 expression in lung adenocarcinoma cells Corinne expression in lung adenocarcinoma cells HALauthormanuscriptinserm-00143972,version1 HAL author manuscript Lung Cancer 56, 2 (2007) 167-74 #12;2 Summary In lung adenocarcinoma, expression of Regulated upon

  1. Relativistic tidal heating of Hamiltonian quasi-local boundary expressions

    E-Print Network [OSTI]

    So, Lau Loi

    2015-01-01T23:59:59.000Z

    Purdue and Favata calculate the tidal heating used certain classical pseudotensors. Booth and Creighton employed the quasi-local mass formalism of Brown and York to demonstrate the same subject. All of them give the result matched with the Newtonian theory. Here we present another Hamiltonian quasi-local boundary expressions and all give the same desired value. This indicates that the tidal heating is unique as Thorne predicted. Moreover, we discovered that the pseudo-tensor method and quasi-local method are fundamentally different.

  2. Relativistic tidal heating of Hamiltonian quasi-local boundary expressions

    E-Print Network [OSTI]

    Lau Loi So

    2015-05-19T23:59:59.000Z

    Purdue and Favata calculate the tidal heating used certain classical pseudotensors. Booth and Creighton employed the quasi-local mass formalism of Brown and York to demonstrate the same subject. All of them give the result matched with the Newtonian theory. Here we present another Hamiltonian quasi-local boundary expressions and all give the same desired value. This indicates that the tidal heating is unique as Thorne predicted. Moreover, we discovered that the pseudo-tensor method and quasi-local method are fundamentally different.

  3. Detecting separate time scales in genetic expression data

    E-Print Network [OSTI]

    Orlando, David A; Brady, Siobhan M; Fink, Thomas M A; Benfey, Philip N; Ahnert, Sebastian E

    2010-06-16T23:59:59.000Z

    experiments. Cell Cycle 2007, 6:478-488. 5. Boyle EI, Weng SA, Gollub J, Jin H, Botstein D, Cherry JM, Sherlock G: GO:: TermFinder-open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms... Orlando et al. BMC Genomics 2010, 11:381 http://www.biomedcentral.com/1471-2164/11/381Open AccessM E T H O D O L O G Y A R T I C L E Methodology articleDetecting separate time scales in genetic expression data David A Orlando1,2, Siobhan M Brady1...

  4. Effects of Acute and Chronic Stress on Immune- and Inflammatory-response Gene Expression in Beef Calves

    E-Print Network [OSTI]

    Terrill, Cooper

    2012-02-14T23:59:59.000Z

    . ........................... 56 18 Amplification plot of gene expression for primer IL-10RB ....................... 57 19 Amplification plot of gene expression for primer IL-12B .......................... 58 20 Amplification plot of gene expression for primer LBP...

  5. Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood

    E-Print Network [OSTI]

    2009-01-01T23:59:59.000Z

    2002). Gene expression profile of human bone marrow stromal2003). Gene expression profile of mouse bone marrow stromalRNA (ncRNA) expression profiles of MSC from match-paired UC

  6. Expression and rearrangement of the ROS1 gene in human glioblastoma cells

    SciTech Connect (OSTI)

    Birchmeier, C.; Sharma, S.; Wigler, M.

    1987-12-01T23:59:59.000Z

    The human ROS1 gene, which possibly encodes a growth factor receptor, was found to be expressed in human tumor cell lines. In a survey of 45 different human cell lines, the authors found ROS1 to be expressed in glioblastoma-derived cell lines at high levels and not to be expressed at all, or expressed at very low levels, in the remaining cell lines. The ROS1 gene was present in normal copy numbers in all cell lines that expressed the gene. However, in one particular glioblastoma line, they detected a potentially activating mutation at the ROS1 locus.

  7. Signal Transduction Pathways that Regulate CAB Gene Expression

    SciTech Connect (OSTI)

    Chory, Joanne

    2006-01-16T23:59:59.000Z

    The process of chloroplast differentiation, involves the coordinate regulation of many nuclear and chloroplast genes. The cues for the initiation of this developmental program are both extrinsic (e.g., light) and intrinsic (cell-type and plastid signals). During this project period, we utilized a molecular genetic approach to select for Arabidopsis mutants that did not respond properly to environmental light conditions, as well as mutants that were unable to perceive plastid damage. These latter mutants, called gun mutants, define two retrograde signaling pathways that regulate nuclear gene expression in response to chloroplasts. A major finding was to identify a signal from chloroplasts that regulates nuclear gene transcription. This signal is the build-up of Mg-Protoporphyrin IX, a key intermediate of the chlorophyll biosynthetic pathway. The signaling pathways downstream of this signal are currently being studied. Completion of this project has provided an increased understanding of the input signals and retrograde signaling pathways that control nuclear gene expression in response to the functional state of chloroplasts. These studies should ultimately influence our abilities to manipulate plant growth and development, and will aid in the understanding of the developmental control of photosynthesis.

  8. Signal Transduction Pathways that Regulate CAB Gene Expression

    SciTech Connect (OSTI)

    Chory, Joanne

    2004-12-31T23:59:59.000Z

    The process of chloroplast differentiation, involves the coordinate regulation of many nuclear and chloroplast genes. The cues for the initiation of this developmental program are both extrinsic (e.g., light) and intrinsic (cell-type and plastid signals). During this project period, we utilized a molecular genetic approach to select for Arabidopsis mutants that did not respond properly to environmental light conditions, as well as mutants that were unable to perceive plastid damage. These latter mutants, called gun mutants, define two retrograde signaling pathways that regulate nuclear gene expression in response to chloroplasts. A major finding was to identify a signal from chloroplasts that regulates nuclear gene transcription. This signal is the build-up of Mg-Protoporphyrin IX, a key intermediate of the chlorophyll biosynthetic pathway. The signaling pathways downstream of this signal are currently being studied. Completion of this project has provided an increased understanding of the input signals and retrograde signaling pathways that control nuclear gene expression in response to the functional state of chloroplasts. These studies should ultimately influence our abilities to manipulate plant growth and development, and will aid in the understanding of the developmental control of photosynthesis.

  9. MEASURING AND EXPRESSING THE PERFORMANCE OF ENERGY STORAGE SYSTEMS

    SciTech Connect (OSTI)

    Schoenwald, David; Conover, David R.

    2013-12-03T23:59:59.000Z

    Until late 2012, there was no uniform methodology to measure and express the performance of energy storage systems (ESS). A void in this area can affect the acceptance of ESS in the marketplace because different systems cannot be equitably compared and ESS cost-benefit analysis may be challenging due to a lack of verified and relevant ESS performance. The lack of such criteria also furthers the probability that each ESS customer or user will make up their own; necessitating “custom validation” to a unique set of criteria each time an ESS is to be considered or installed. To address this need and foster the acceptance of ESS, the U.S. Department of Energy’s (DOE) Energy Storage Systems Program facilitated the development of a protocol to measure and express ESS performance and is supporting its updating, enhancement and use in formal consensus standards development. Of particular interest is the development of the document through an open and transparent process that saved considerable time.

  10. Polarized photoluminescence and absorption in A-plane InN films Jayeeta Bhattacharyya, Sandip Ghosh,a

    E-Print Network [OSTI]

    Ghosh, Sandip

    infrared wavelength region which includes important areas of fiber-optic communication and solar cells edge for E c is shifted to higher energy by 20 meV relative to E c. Optical polarization anisotropy supporting a high value for Eg suggest that the ob- served low energy photoluminescence PL and absorption

  11. Strain effects in group-III nitrides: Deformation potentials for AlN, GaN, and InN

    E-Print Network [OSTI]

    in quantum cryptography5 or in photocatalysis6 are being explored. Applications in solid state lighting

  12. Human AZU-1 gene, variants thereof and expressed gene products

    DOE Patents [OSTI]

    Chen, Huei-Mei; Bissell, Mina

    2004-06-22T23:59:59.000Z

    A human AZU-1 gene, mutants, variants and fragments thereof. Protein products encoded by the AZU-1 gene and homologs encoded by the variants of AZU-1 gene acting as tumor suppressors or markers of malignancy progression and tumorigenicity reversion. Identification, isolation and characterization of AZU-1 and AZU-2 genes localized to a tumor suppressive locus at chromosome 10q26, highly expressed in nonmalignant and premalignant cells derived from a human breast tumor progression model. A recombinant full length protein sequences encoded by the AZU-1 gene and nucleotide sequences of AZU-1 and AZU-2 genes and variant and fragments thereof. Monoclonal or polyclonal antibodies specific to AZU-1, AZU-2 encoded protein and to AZU-1, or AZU-2 encoded protein homologs.

  13. A graphical environment to express the semantics of control systems

    E-Print Network [OSTI]

    Wang, Timothy; Feron, E

    2011-01-01T23:59:59.000Z

    We present the concept of a unified graphical environment for expressing the semantics of control systems. The graphical control system design environment in Simulink already allows engineers to insert a variety of assertions aimed the verification and validation of the control software. We propose extensions to a Simulink-like environment's annotation capabilities to include formal control system stability, performance properties and their proofs. We provide a conceptual description of a tool, that takes in a Simulink-like diagram of the control system as the input, and generates a graphically annotated control system diagram as the output. The annotations can either be inserted by the user or generated automatically by a third party control analysis software such as IQC$\\beta$ or $\\mu$-tool. We finally describe how the graphical representation of the system and its properties can be translated to annotated programs in a programming language used in verification and validation such as Lustre or C.

  14. Biphasic Hoxd Gene Expression in Shark Paired Fins Reveals an Ancient Origin of the Distal Limb Domain

    E-Print Network [OSTI]

    Cohn, Martin

    Biphasic Hoxd Gene Expression in Shark Paired Fins Reveals an Ancient Origin of the Distal Limb of expression occurs at later stages of shark fin development, in which Hoxd12 and Hoxd13 are re-expressed along GJ, Cohn MJ (2007) Biphasic Hoxd Gene Expression in Shark Paired Fins Reveals an Ancient Origin

  15. Circadian oscillation of starch branching enzyme gene expression in the sorghum endosperm

    SciTech Connect (OSTI)

    Mutisya, J.; Sun, C.; Jansson, C.

    2009-08-31T23:59:59.000Z

    Expression of the three SBE genes, encoding starch branching enzymes, in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle. Remarkably, the oscillation in SBE expression was maintained in cultured spikes after a 48-h dark treatment, also when fed a continuous solution of sucrose or abscisic acid. Our findings suggest that the rhythmicity in SBE expression in the endosperm is independent of cues from the photosynthetic source and that the oscillator resides within the endosperm itself.

  16. High density growth of T7 expression strains with auto-induction option

    DOE Patents [OSTI]

    Studier, F. William

    2010-04-27T23:59:59.000Z

    Methods for promoting and suppressing auto-induction of transcription of cloned DNA in cultures of T7 expression strains are disclosed.

  17. Gene connectivity, function, and sequence conservation: predictions from modular yeast co-expression networks

    E-Print Network [OSTI]

    Carlson, Marc R J; Zhang, Bin; Fang, Zixing; Mischel, Paul S; Horvath, Steve; Nelson, Stanley F

    2006-01-01T23:59:59.000Z

    connectivity would lead to information loss. The adjacencygene co-expression information and, leads to results thatgenes does not lead to major information loss for the key

  18. Hierarchical Dirichlet Process-Based Models For Discovery of Cross-species Mammalian Gene Expression

    E-Print Network [OSTI]

    Gerber, Georg K.

    2007-07-06T23:59:59.000Z

    An important research problem in computational biology is theidentification of expression programs, sets of co-activatedgenes orchestrating physiological processes, and thecharacterization of the functional breadth of these ...

  19. There are no classes or examinations on Bank Holidays (4 and 25 Mid-Semester Study Period (First Semester)

    E-Print Network [OSTI]

    Glendinning, Paul

    .3 SCHOOL COMMUNICATION The main Reception Area in the Alan Turing Building is just inside the main entrance fortnightly and important messages are displayed on the OneLan Screen in the foyer of the Alan Turing Building. There are student notice boards in the Alan Turing Building on the walls inside the Mathematics Undergraduate Work

  20. Estimated Savings from Turning Off Unnecessary Lights at the Langford Architecture Center During the 1996 Christmas Holidays

    E-Print Network [OSTI]

    Soebarto, V. I.; Haberl, J. S.; Degelman, L. O.

    1997-01-01T23:59:59.000Z

    lights been left on, the building would have consumed 100 kW more electricity every hour. The total electricity savings was about 31,200 kWh over 13 days, which is equivalent to a total cost saving of $936.00. If the College continues to turn off...

  1. San Antonio HolidayFeaturing 4 Nights in San Antonio December 4-8, 2013 (5 days)

    E-Print Network [OSTI]

    Day 4: LBJ Ranch - Fredericksburg - Luminarias Today travel to the Hill Country of Texas and take Antonio. Later wander the Riverwalk to experience the Fiesta de las Luminarias, which features thousands ·Riverwalk Cruise ·Hill Country ·LBJ Ranch ·Fredericksburg ·Fiesta de las Luminarias ·4 Nights at One Hotel

  2. ETUDE DE L'EXPRESSION DES KINASES AURORA DANS LE CARCINOME A CELLULES RENALES

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    ETUDE DE L'EXPRESSION DES KINASES AURORA DANS LE CARCINOME A CELLULES RENALES Romain Mathieu rénales, kinases Aurora, thérapies ciblées, envahissement ganglionnaire Nombre de mots total : 2092 Nombre OF THE EXPRESSION OF AURORA KINASES IN RENAL CELL CARCINOMA Romain Mathieu(a), Jean-Jacques Patard(a,b), Nathalie

  3. Prokupek 2008 THE ANALYSIS OF GENES EXPRESSED IN THE SPERM STORAGE

    E-Print Network [OSTI]

    Moriyama, Etsuko

    Prokupek 2008 THE ANALYSIS OF GENES EXPRESSED IN THE SPERM STORAGE ORGANS OF DROSOPHILA and Learning Company. #12;Prokupek 2008 THE ANALYSIS OF GENES EXPRESSED IN THE SPERM STORAGE ORGANS reproductive system. Sperm storage organs are important to the reproductive success of both males and females

  4. Early Embryonic Expression of Ion Channels and Pumps in Chick and Xenopus Development

    E-Print Network [OSTI]

    Levin, Michael

    Early Embryonic Expression of Ion Channels and Pumps in Chick and Xenopus Development JOSHUA during embryonic morphogenesis. Al- though the expression of ion channel and pump genes, which on the distribution and function of specific channels and pumps in early embryogenesis. To provide a necessary basis

  5. Automatic Construction of Large-Scale Regular Expression Matching Engines on FPGA

    E-Print Network [OSTI]

    Prasanna, Viktor K.

    Automatic Construction of Large-Scale Regular Expression Matching Engines on FPGA Yi-Hua E. Yang@usc.edu, prasanna@usc.edu Abstract--We present algorithms for implementing large-scale regular expression matching (REM) on FPGA. Based on the proposed algorithms, we develop tools that first transform regular

  6. Microfluidic single cell real-time PCR for comparative analysis of gene expression patterns

    E-Print Network [OSTI]

    Quake, Stephen R.

    Microfluidic single cell real-time PCR for comparative analysis of gene expression patterns collection. We present here a high-content microfluidic real-time platform as a powerful tool expression; dynamic assay; microfluidic; pluripotent INTRODUCTION Novel genomic technologies have paved

  7. Phosphate stimulates Matrix Gla Protein expression in chondrocytes through the ERK signaling pathway

    E-Print Network [OSTI]

    Boyer, Edmond

    1 Phosphate stimulates Matrix Gla Protein expression in chondrocytes through the ERK signaling. Email: Jerome.guicheux@nantes.inserm.fr Short title Phosphate stimulates MGP expression via ERK-regulated kinase 1 and 2 (ERK1/2) was performed in rib organ cultures from newborn mice. Results indicated that Pi

  8. Synthesis and Recognition of Facial Expressions in Virtual 3D Views Lukasz Zalewski and Shaogang Gong

    E-Print Network [OSTI]

    Gong, Shaogang

    Gong Department of Computer Science, Queen Mary, University of London, E1 4NS, UK [lukas to synthesise existing sequences with different expressions during the speaking process. Gong et al.[8] used to incorporate expression changes under large pose and scale variations. As Gong et al.[8] point out, la

  9. Protein Expression and PuriWcation 54 (2007) 5465 www.elsevier.com/locate/yprep

    E-Print Network [OSTI]

    Lebendiker, Mario

    2007-01-01T23:59:59.000Z

    Wcation, renaturation and activation of Wsh myostatin expressed in Escherichia coli: Facilitation of refolding the precursor myostatin, also yielding the N-terminal prodomain. We expressed in Escherichia coli three forms and activity inhibition by myostatin prodomain Bruria Funkenstein ¤ , Yanai Rebhan Department of Marine Biology

  10. Dartmouth College Computer Science Technical Report TR2004-503 Enhancing Expressiveness of Speech

    E-Print Network [OSTI]

    Dartmouth College Computer Science Technical Report TR2004-503 Enhancing Expressiveness of Speech for expressiveness. When text is entered into the program, the Festival Text-to-Speech engine creates a speech file be obtained for speech dictated into a microphone using Microsoft SAPI and the CSLU Toolkit. Once lip

  11. Expressing and computing passage time measures of GSPN models with HASL

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Expressing and computing passage time measures of GSPN models with HASL Elvio Gilberto Amparore1 measures in (Tagged) GSPNs using the Hybrid Automata Stochastic Logic (HASL) and the statistical model), formally express them in HASL terms and assess them by means of simulation in the COSMOS tool. The interest

  12. Project EARTH-12-SHELL1: Global expression of climatic and palaeoceanographic events in black shales

    E-Print Network [OSTI]

    Henderson, Gideon

    geochronology, the expression of the global Toarcian Oceanic Anoxic Event (T- OAE) ­ a major black shale eventProject EARTH-12-SHELL1: Global expression of climatic and palaeoceanographic events in black shales: generation of new high-resolution records from the Jurassic of the Neuquen Basin, Argentina

  13. Expression of a Tensor Commutation Matrix in Terms of the Generalized Gell-Mann Matrices

    E-Print Network [OSTI]

    Rakotonirina Christian

    2005-11-21T23:59:59.000Z

    We have expressed the tensor commutation matrix n\\otimes n as linear combination of the tensor products of the generalized Gell-Mann matrices. The tensor commutation matrices 3\\otimes 2 and 2\\otimes 3 have been expressed in terms of the classical Gell-Mann matrices and the Pauli matrices.

  14. A Simple Neural Network Models Categorical Perception of Facial Expressions Curtis Padgett and Garrison W. Cottrell

    E-Print Network [OSTI]

    Cottrell, Garrison W.

    facial expressions is compared with human subjects over a set of experiments using interpolated imagery. The experiments for both the human subjects and neural networks make use of in­ terpolations of facial expressions in materials between those used in the human subjects experiments [Young et al., 1997] and our materials

  15. Factory Express a New Mexico Service Disabled Veteran-Owned Company

    E-Print Network [OSTI]

    Factory Express a New Mexico Service Disabled Veteran-Owned Company ... a New Mexico small business the company.'" Factory Express is the exclusive provider in New Mexico of service and sales for the type Kirtland Air Force Base in Albuquerque, New Mexico. Office size? Small. Staff size? Small. Budget? Small

  16. Correlating EGFR Expression with Receptor-Binding Properties and Internalization of

    E-Print Network [OSTI]

    Pike, Linda J.

    tumors, and small-animal PET/CT of EGFR-expressing CaSki tumors sug- gests potential for PET/CT of EGFR potential as an agent for measuring EGFR concentration by PET imaging in cervical cancer tumors. Methods- bearing mice with small-animal PET was performed using the highest-expressing cervical cancer cell line

  17. Gene expression divergence and nucleotide differentiation between males of different color morphs and mating

    E-Print Network [OSTI]

    Gene expression divergence and nucleotide differentiation between males of different color morphs sequencing, it is possible to obtain data on both nucleotide sequence variation and gene expression. We have between males, but many genetic markers showed nucleotide differentiation between different color morphs

  18. Spatial Segregation of Virulence Gene Expression during Acute Enteric Infection with Salmonella enterica serovar Typhimurium 

    E-Print Network [OSTI]

    Laughlin, Richard C.; Knodler, Leigh A.; Barhoumi, Roula; Payne, H. Ross; Wu, Jing; Gomez, Gabriel; Pugh, Roberta; Lawhon, Sara D.; Bä umler, Andreas J.; Steele-Mortimer, Olivia; Adams, L. Garry

    2014-02-04T23:59:59.000Z

    localization and temporal expression of T3SS-1 and T3SS-2 using fluorescent single-cell reporters in a bovine, ligated ileal loop model of infection. We observed that the majority of bacteria at 2 h postinfection are flagellated, express T3SS-1 but not T3SS-2...

  19. Phyllody of faba bean in the Sudan. I. Progress of symptom expression, host range and transmis-

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Phyllody of faba bean in the Sudan. I. Progress of symptom expression, host range and transmis bean phyllody was followed in a local susceptible cultivar, Hudeiba 72, graft-inoculated from naturally and slow development of disease, sometimes partial recovery. Symptom expression in faba bean plants

  20. Original article Expression and activity of N-myristoyltransferase in lung

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Original article Expression and activity of N-myristoyltransferase in lung inflammation of cattle and inflamed lungs of the cattle are unknown. Therefore, we investigated the expression and activity of NMT in a bovine model of lung inflammation induced with Mannheimia hemolytica and in vitro in neutrophils

  1. The orphan nuclear receptor Nur77 regulates decidual prolactin expression in human endometrial stromal cells

    SciTech Connect (OSTI)

    Jiang, Yue; Hu, Yali; Zhao, Jing; Zhen, Xin [Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008 (China)] [Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008 (China); Yan, Guijun, E-mail: yanguijun33@gmail.com [Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008 (China)] [Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008 (China); Sun, Haixiang, E-mail: stevensunz@163.com [Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008 (China)] [Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008 (China)

    2011-01-14T23:59:59.000Z

    Research highlights: {yields} Decidually produced PRL plays a key role during pregnancy. {yields} Overexpression of Nur77 increased PRL mRNA expression and enhanced decidual PRL promoter activity. {yields} Knockdown of Nur77 decreased decidual PRL secretion induced by 8-Br-cAMP and MPA. {yields} Nur77 is a novel transcription factor that plays an active role in decidual prolactin expression. -- Abstract: Prolactin (PRL) is synthesized and released by several extrapituitary tissues, including decidualized stromal cells. Despite the important role of decidual PRL during pregnancy, little is understood about the factors involved in the proper regulation of decidual PRL expression. Here we present evidence that the transcription factor Nur77 plays an active role in decidual prolactin expression in human endometrial stromal cells (hESCs). Nur77 mRNA expression in hESCs was significantly increased after decidualization stimulated by 8-Br-cAMP and medroxyprogesterone acetate (MPA). Adenovirus-mediated overexpression of Nur77 in hESCs markedly increased PRL mRNA expression and enhanced decidual PRL promoter (dPRL/-332Luc) activity in a concentration-dependent manner. Furthermore, knockdown of Nur77 in hESCs significantly decreased decidual PRL promoter activation and substantially attenuated PRL mRNA expression and PRL secretion (P < 0.01) induced by 8-Br-cAMP and MPA. These results demonstrate that Nur77 is a novel transcription factor that contributes significantly to the regulation of prolactin gene expression in human endometrial stromal cells.

  2. Phenotypic plasticity in opsin expression in a butterfly compound eye complements sex role

    E-Print Network [OSTI]

    Monteiro, Antónia

    Phenotypic plasticity in opsin expression in a butterfly compound eye complements sex role reversal-2148/12/232 #12;RESEARCH ARTICLE Open Access Phenotypic plasticity in opsin expression in a butterfly compound eye* Abstract Background: Animals often display phenotypic plasticity in morphologies and behaviors that result

  3. Expressive Gestures Displayed by a Humanoid Robot during a Storytelling Application

    E-Print Network [OSTI]

    Pelachaud, Catherine

    Expressivity is an important factor in communication, be human- human, human-virtual agent or human-robot in the expression of emotion [30, 16]. Our idea is to endow a humanoid robot with human communicative capabilities. Nao is a humanoid robot able to display body movement and ges- ture. It can talk and interact

  4. Studies on gene expression profiling in JB6 cells susceptible and resistant to tumor promoter induced neoplastic transformation and regulation of gene expression at the AP-1 DNA binding site 

    E-Print Network [OSTI]

    Samuel, Shaija

    2005-11-01T23:59:59.000Z

    Gene expression underlies all important biological processes in a cell and mis-regulated gene expression plays a causal or contributory role in several diseases including cancers. Towards identifying molecular determinants ...

  5. Studies on gene expression profiling in JB6 cells susceptible and resistant to tumor promoter induced neoplastic transformation and regulation of gene expression at the AP-1 DNA binding site

    E-Print Network [OSTI]

    Samuel, Shaija

    2005-11-01T23:59:59.000Z

    and resistance to tumor promoter induced neoplastic transformation, we analyzed the gene expression profile differences among tumor promoter TPA treated and untreated mouse epidermal JB6 cells by means of cDNA microarray analyses. The expression patterns...

  6. Differential expression of nanog1 and nanogp8 in colon cancer cells

    SciTech Connect (OSTI)

    Ishiguro, Tatsuya; Sato, Ai; Ohata, Hirokazu; Sakai, Hiroaki [Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Nakagama, Hitoshi, E-mail: hnakagam@ncc.go.jp [Division of Cancer Development System, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Cancer Development System, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Okamoto, Koji, E-mail: kojokamo@ncc.go.jo [Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Cancer Differentiation, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)

    2012-02-10T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Nanog is expressed in a majority of colon cancer cell lines examined. Black-Right-Pointing-Pointer Both nanog1 and nanogp8 are expressed in colon cancer cells with varying ratios. Black-Right-Pointing-Pointer Nanog mediates cell proliferation of colon cancer cells. Black-Right-Pointing-Pointer Nanog predominantly localizes in cytoplasm of colon cancer cells. -- Abstract: Nanog, a homeodomain transcription factor, is an essential regulator for promotion of self-renewal of embryonic stem cells and inhibition of their differentiation. It has been demonstrated that nanog1 as well as nanogp8, a retrogene of nanog1, is preferentially expressed in advanced stages of several types of cancer, suggesting their involvement during cancer progression. Here, we investigated the expression of Nanog in well-characterized colon cancer cell lines. Expression of Nanog was detectable in 5 (HCT116, HT29, RKO, SW48, SW620) out of seven cell lines examined. RNA expression analyses of nanog1 and nanogp8 indicated that, while nanog1 was a major form in SW620 as well as in teratoma cells Tera-2, nanogp8 was preferentially expressed in HT29 and HCT116. In accordance with this, shRNA-mediated knockdown of nanog1 caused the reduction of Nanog in SW620 but not in HT29. Inhibition of Nanog in SW620 cells negatively affected cell proliferation and tumor formation in mouse xenograft. Biochemical subcellular fractionation and immunostaining analyses revealed predominant localization of Nanog in cytoplasm in SW620 and HT29, while it was mainly localized in nucleus in Tera-2. Our data indicate that nanog1 and nanogp8 are differentially expressed in colon cancer cells, and suggest that their expression contributes to proliferation of colon cancer cells.

  7. Gene expression profiling in Ishikawa cells: A fingerprint for estrogen active compounds

    SciTech Connect (OSTI)

    Boehme, Kathleen; Simon, Stephanie [Merck KGaA, Merck Serono, NCD/Toxicology, Early and Explanatory Toxicology, 64271 Darmstadt (Germany); Mueller, Stefan O. [Merck KGaA, Merck Serono, NCD/Toxicology, Early and Explanatory Toxicology, 64271 Darmstadt (Germany)], E-mail: stefan.o.mueller@merck.de

    2009-04-01T23:59:59.000Z

    Several anthropogenous and naturally occurring substances, referred to as estrogen active compounds (EACs), are able to interfere with hormone and in particular estrogen receptor signaling. EACs can either cause adverse health effects in humans and wildlife populations or have beneficial effects on estrogen-dependent diseases. The aim of this study was to examine global gene expression profiles in estrogen receptor (ER)-proficient Ishikawa plus and ER-deficient Ishikawa minus endometrial cancer cells treated with selected well-known EACs (Diethylstilbestrol, Genistein, Zearalenone, Resveratrol, Bisphenol A and o,p'-DDT). We also investigated the effect of the pure antiestrogen ICI 182,780 (ICI) on the expression patterns caused by these compounds. Transcript levels were quantified 24 h after compound treatment using Illumina BeadChip Arrays. We identified 87 genes with similar expression changes in response to all EAC treatments in Ishikawa plus. ICI lowered the magnitude or reversed the expression of these genes, indicating ER dependent regulation. Apart from estrogenic gene regulation, Bisphenol A, o,p'-DDT, Zearalenone, Genistein and Resveratrol displayed similarities to ICI in their expression patterns, suggesting mixed estrogenic/antiestrogenic properties. In particular, the predominant antiestrogenic expression response of Resveratrol could be clearly distinguished from the other test compounds, indicating a distinct mechanism of action. Divergent gene expression patterns of the phytoestrogens, as well as weaker estrogenic gene expression regulation determined for the anthropogenous chemicals Bisphenol A and o,p'-DDT, warrants a careful assessment of potential detrimental and/or beneficial effects of EACs. The characteristic expression fingerprints and the identified subset of putative marker genes can be used for screening chemicals with an unknown mode of action and for predicting their potential to exert endocrine disrupting effects.

  8. Sex-based differences in gene expression in hippocampus following postnatal lead exposure

    SciTech Connect (OSTI)

    Schneider, J.S., E-mail: jay.schneider@jefferson.edu; Anderson, D.W.; Sonnenahalli, H.; Vadigepalli, R.

    2011-10-15T23:59:59.000Z

    The influence of sex as an effect modifier of childhood lead poisoning has received little systematic attention. Considering the paucity of information available concerning the interactive effects of lead and sex on the brain, the current study examined the interactive effects of lead and sex on gene expression patterns in the hippocampus, a structure involved in learning and memory. Male or female rats were fed either 1500 ppm lead-containing chow or control chow for 30 days beginning at weaning.Blood lead levels were 26.7 {+-} 2.1 {mu}g/dl and 27.1 {+-} 1.7 {mu}g/dl for females and males, respectively. The expression of 175 unique genes was differentially regulated between control male and female rats. A total of 167 unique genes were differentially expressed in response to lead in either males or females. Lead exposure had a significant effect without a significant difference between male and female responses in 77 of these genes. In another set of 71 genes, there were significant differences in male vs. female response. A third set of 30 genes was differentially expressed in opposite directions in males vs. females, with the majority of genes expressed at a lower level in females than in males. Highly differentially expressed genes in males and females following lead exposure were associated with diverse biological pathways and functions. These results show that a brief exposure to lead produced significant changes in expression of a variety of genes in the hippocampus and that the response of the brain to a given lead exposure may vary depending on sex. - Highlights: > Postnatal lead exposure has a significant effect on hippocampal gene expression patterns. > At least one set of genes was affected in opposite directions in males and females. > Differentially expressed genes were associated with diverse biological pathways.

  9. Ethanol increases matrix metalloproteinase-12 expression via NADPH oxidase-dependent ROS production in macrophages

    SciTech Connect (OSTI)

    Kim, Mi Jin; Nepal, Saroj; Lee, Eung-Seok; Jeong, Tae Cheon [College of Pharmacy, Yeungnam University, Gyeongsanbuk-do 712-749 (Korea, Republic of); Kim, Sang-Hyun [Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Park, Pil-Hoon, E-mail: parkp@yu.ac.kr [College of Pharmacy, Yeungnam University, Gyeongsanbuk-do 712-749 (Korea, Republic of)

    2013-11-15T23:59:59.000Z

    Matrix metalloproteinase-12 (MMP-12), an enzyme responsible for degradation of extracellular matrix, plays an important role in the progression of various diseases, including inflammation and fibrosis. Although most of those are pathogenic conditions induced by ethanol ingestion, the effect of ethanol on MMP-12 has not been explored. In the present study, we investigated the effect of ethanol on MMP-12 expression and its potential mechanisms in macrophages. Here, we demonstrated that ethanol treatment increased MMP-12 expression in primary murine peritoneal macrophages and RAW 264.7 macrophages at both mRNA and protein levels. Ethanol treatment also significantly increased the activity of nicotinamide adenine dinucleotide (NADPH) oxidase and the expression of NADPH oxidase-2 (Nox2). Pretreatment with an anti-oxidant (N-acetyl cysteine) or a selective inhibitor of NADPH oxidase (diphenyleneiodonium chloride (DPI)) prevented ethanol-induced MMP-12 expression. Furthermore, knockdown of Nox2 by small interfering RNA (siRNA) prevented ethanol-induced ROS production and MMP-12 expression in RAW 264.7 macrophages, indicating a critical role for Nox2 in ethanol-induced intracellular ROS production and MMP-12 expression in macrophages. We also showed that ethanol-induced Nox2 expression was suppressed by transient transfection with dominant negative I?B-? plasmid or pretreatment with Bay 11-7082, a selective inhibitor of NF-?B, in RAW 264.7 macrophages. In addition, ethanol-induced Nox2 expression was also attenuated by treatment with a selective inhibitor of p38 MAPK, suggesting involvement of p38 MAPK/NF-?B pathway in ethanol-induced Nox2 expression. Taken together, these results demonstrate that ethanol treatment elicited increase in MMP-12 expression via increase in ROS production derived from Nox2 in macrophages. - Highlights: • Ethanol increases ROS production through up-regulation of Nox2 in macrophages. • Enhanced oxidative stress contributes to ethanol-induced MMP-12 expression. • p38 MAPK/NF-?B signaling pathway modulates ethanol-induced Nox2 expression.

  10. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen P.

    2006-10-17T23:59:59.000Z

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  11. RNA binding protein and binding site useful for expression of recombinant molecules

    DOE Patents [OSTI]

    Mayfield, Stephen (Cardiff, CA)

    2000-01-01T23:59:59.000Z

    The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

  12. Characterizing cell-cycle as a global regulator of stochastic transcription and noisy gene expression in S. cerevisiae

    E-Print Network [OSTI]

    Quinn, Katie J. (Katie Julia)

    2014-01-01T23:59:59.000Z

    Even in the same environment, genetically identical cells can exhibit remarkable variability, or noise, in gene expression. This expression noise impacts the function of gene regulatory networks, depending on its origins. ...

  13. Systematic analysis of the role of differential expression of microRNAs associated with cell death decisions

    E-Print Network [OSTI]

    Guillén, Nancy, Ph. D. Massachusetts Institute of Technology

    2014-01-01T23:59:59.000Z

    The link between abnormal microRNA expression and cancer has been widely reported. However, little is known about the relationships between temporal microRNA expression and changes in cell behavior. To better understand ...

  14. Co-culture of mechanically injured cartilage with joint capsule tissue alters chondrocyte expression patterns and increases ADAMTS5 production

    E-Print Network [OSTI]

    Grodzinsky, Alan J.

    We studied changes in chondrocyte gene expression, aggrecan degradation, and aggrecanase production and activity in normal and mechanically injured cartilage co-cultured with joint capsule tissue. Chondrocyte expression ...

  15. Expression of Stromal-Derived Factor-1 Is Decreased by IL-1 and TNF and in Dermal Wound Healing1

    E-Print Network [OSTI]

    Springer, Timothy A.

    A. Springer2 * Stromal-derived factor-1 (SDF-1) is a CXC chemokine that is believed fibroblasts and vessels of noninflamed tissues express SDF-1. Unexpectedly, we found that expression of SDF-1 is regulated by inflammation. Expression of SDF-1 by primary cultures of human gingival fibroblasts is potently

  16. New trends in protein expression In order to benefit from the wealth of genomics data and to

    E-Print Network [OSTI]

    Sussman, Joel L.

    systems. These include parallel construct design, new and more robust expression systems, modifications to support the pressing requirements of the research pipeline. However, protein expression is not only to the expres- sion constructs, introduction of new and/or improved prokaryotic and eukaryotic expression

  17. Prostaglandin E2 regulates amyloid precursor protein expression via the EP2 receptor in cultured rat microglia

    E-Print Network [OSTI]

    Wurtman, Richard

    to be positively coupled to cAMP production. Stimulation of the EP2 receptor with butaprost increased APP) expression in cultured rat microglia. PGE2 treatment significantly increased the expression of APP with forskolin also increased APP expression. Co-treatment of microglia with PGE2 and the PKA inhibitor H- 89

  18. Genomic Analysis of Parent-of-Origin Allelic Expression in Arabidopsis thaliana Seeds

    E-Print Network [OSTI]

    Gehring, Mary

    Differential expression of maternally and paternally inherited alleles of a gene is referred to as gene imprinting, a form of epigenetic gene regulation common to flowering plants and mammals. In plants, imprinting primarily ...

  19. Transcriptional regulation of seed-specific gene expression - from PvALF/ ABI3 to phaseolin

    E-Print Network [OSTI]

    Ng, Wang Kit

    2006-10-30T23:59:59.000Z

    the inactive state in transgenic Arabidopsis leaves was accomplished by ectopic expression of the transcription factor PvALF (Phaseolus vulgaris ABI3-like factor), and application of abscisic acid (ABA). PvALF belongs to a family of seed...

  20. Investigations of Heat Shock Protein Expression in the Swordtail Fishes Xiphophorus birchmanni and X. malinche

    E-Print Network [OSTI]

    Meaders, Ashley M.

    2011-08-04T23:59:59.000Z

    and fish that underwent an acute heat stress experiment. In both cases, the population that experienced very little environmental heat stress expressed significantly less Hsps than the population that experienced much greater natural thermal variation...

  1. Microarray analysis of microRNA expression in the developing mammalian brain

    E-Print Network [OSTI]

    Miska, Eric A

    Background: MicroRNAs are a large new class of tiny regulatory RNAs found in nematodes, plants, insects and mammals. MicroRNAs are thought to act as post-transcriptional modulators of gene expression. In invertebrates ...

  2. Extensive changes in DNA methylation are associated with expression of mutant huntingtin

    E-Print Network [OSTI]

    Ng, Christopher W.

    The earliest stages of Huntington disease are marked by changes in gene expression that are caused in an indirect and poorly understood manner by polyglutamine expansions in the huntingtin (HTT) protein. To explore the ...

  3. Microbial metatranscriptomics : towards understanding microbial gene expression and regulation in natural habitats

    E-Print Network [OSTI]

    Shi, Yanmei, Ph. D. Massachusetts Institute of Technology

    2011-01-01T23:59:59.000Z

    Metagenomic research has paved the way for a comprehensive understanding of the microbial gene parts list in nature, but a full understanding of microbial gene expression, regulation, and ecology remains a challenge. In ...

  4. A Novel Approach to Determine Normal Variation in Gene Expression Data

    E-Print Network [OSTI]

    Zaki, Mohammed Javeed

    among identical mice, thus enabling a comprehensive database of normal variations in gene expression EIA-0103708 under-appreciated problem in microarray analysis is the in- cidence of microarrays

  5. Gene expression profiling in the lungs of pigs with different susceptibilities to Glasser's disease

    E-Print Network [OSTI]

    Wilkinson, Jamie M; Sargent, Carole A; Galina-Pantoja, Lucina; Tucker, Alexander W

    2010-07-29T23:59:59.000Z

    microarray to identify genes that were differentially expressed (DE) in the lungs of colostrum-deprived animals previously characterized as being either 'Fully Resistant' (FR) or 'Susceptible' to infection by H. parasuis in a bacterial challenge experiment...

  6. Heterogeneity of tumor-induced gene expression changes in the human metabolic network

    E-Print Network [OSTI]

    Hu, Jie

    Reprogramming of cellular metabolism is an emerging hallmark of neoplastic transformation. However, it is not known how the expression of metabolic genes in tumors differs from that in normal tissues, or whether different ...

  7. Design criteria for purposefully ambiguous expression : proposal for a theater / performing arts school in Kenmore Square

    E-Print Network [OSTI]

    Verhulst, Catharina A

    1986-01-01T23:59:59.000Z

    Inclusion of zones which possess sufficient ambiguity to provide multiple use, character and meaning, generating an environment supporting freedom of interpretation and expression, is explored in this thesis. Conceptually, ...

  8. A gestural media framework : tools for expressive gesture recognition and mapping in rehearsal and performance

    E-Print Network [OSTI]

    Jessop, Elena Naomi

    2010-01-01T23:59:59.000Z

    As human movement is an incredibly rich mode of communication and expression, performance artists working with digital media often use performers' movement and gestures to control and shape that digital media as part of a ...

  9. Proceedings of the Sudden Oak Death Third Science Symposium Global Gene Expression Profiles of

    E-Print Network [OSTI]

    Standiford, Richard B.

    , United States Department of Agriculture, Corvallis, OR. #12;Proceedings of the Sudden Oak Death Third Science Symposium 435 Global Gene Expression Profiles types if they were statistically significant P=0.05 after false discovery rate correction and resulted

  10. Genomic neighbourhood and the regulation of gene expression Genomic neighbourhood and transcriptional regulation

    E-Print Network [OSTI]

    Babu, M. Madan

    Genomic neighbourhood and the regulation of gene expression Genomic neighbourhood and transcriptional regulation Subhajyoti De and M. Madan Babu MRC Laboratory of Molecular Biology, Hills Road..................................................................................................................................................................................1 2. Genomic neighbourhood and its influence on gene regulation

  11. Spatial patterning of gene expression using surface-immobilized recombinant adenovirus

    E-Print Network [OSTI]

    Chen, Christopher S.

    organization of cells but also control of their gene expression patterns. We report a method to generate (Serls et al. 2005). By controlling which and how much mRNA and protein is produced, one can regulate

  12. antigen-binding protein expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cells before meiosis. After Smith, Steven E. 147 Reduced 293T cell susceptibility to acrolein due to aldose reductase-like-1 protein expression CiteSeer Summary: Acrolein is a...

  13. apoptosis-related protein expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    22 23 24 25 Next Page Last Page Topic Index 141 Reduced 293T cell susceptibility to acrolein due to aldose reductase-like-1 protein expression CiteSeer Summary: Acrolein is a...

  14. Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters

    E-Print Network [OSTI]

    O’Keefe, David D; Thomas, Sean R; Bolin, Kelsey; Griggs, Ellen; Edgar, Bruce A; Buttitta, Laura A

    2012-01-01T23:59:59.000Z

    program to per- form k-means clustering, imposing values ofof expression for each k-means cluster and Supple- mentalthe genes within each k-means cluster, and ordered the

  15. E-Print Network 3.0 - anhydrase ix expression Sample Search Results

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ix expression Page: << < 1 2 3 4 5 > >> 1 Prokaryotic carbonic anhydrases Kerry S. Smith *, James G. Ferry Summary: , growth inhibition by cyanate of a cynS deletion strain...

  16. Induction of murine CYP1A2 gene expression by acenaphthylene

    E-Print Network [OSTI]

    Miggins, John Wesley

    1996-01-01T23:59:59.000Z

    Acenaphthylene and related polynuclear aromatic hydrocarbons (PAH) are industrial combustion by-products which are also found in cooked foods. Recent studies in this laboratory have shown that acenaphthylene induces Cypla2 gene expression in mice...

  17. Anti-Cancer Drugs Elicit Re-Expression of UDP-Glucuronosyltransferases in Melanoma Cells

    E-Print Network [OSTI]

    Dellinger, Ryan W; Matundan, Harry H; Ahmed, Amelia S; Duong, Priscilla H; Meyskens, Frank L; Smalley, Keiran

    2012-01-01T23:59:59.000Z

    Re-Expression of UGTs in Melanoma Acknowledgments AuthorC, Marais R (2007) Melanoma biology and new targetedThe genetics of malignant melanoma: lessons from mouse and

  18. Anti-cancer drugs elicit re-expression of UDP-glucuronosyltransferases in melanoma cells.

    E-Print Network [OSTI]

    Dellinger, Ryan W; Matundan, Harry H; Ahmed, Amelia S; Duong, Priscilla H; Meyskens, Frank L Jr

    2012-01-01T23:59:59.000Z

    Re-Expression of UGTs in Melanoma Acknowledgments AuthorC, Marais R (2007) Melanoma biology and new targetedThe genetics of malignant melanoma: lessons from mouse and

  19. Expressiveness of XSDs: from practice to theory, there and back again

    E-Print Network [OSTI]

    Schwentick, Thomas

    Expressiveness of XSDs: from practice to theory, there and back again Geert Jan Bex Limburgs in DTDs: like namespaces, import facilities, built in basic types, keys, and also the ability to use

  20. Expressiveness of XSDs: from Practice to Theory, There and Back Again

    E-Print Network [OSTI]

    Martens, Wim

    Expressiveness of XSDs: from Practice to Theory, There and Back Again Geert Jan Bex Limburgs. . DTDs such as namespaces, import facilities, built in basic types, keys, and also the ability to use

  1. NANO EXPRESS A Study on Field Emission Characteristics of Planar Graphene

    E-Print Network [OSTI]

    Yang, Eui-Hyeok

    NANO EXPRESS A Study on Field Emission Characteristics of Planar Graphene Layers Obtained from on field emission characteristics of individual graphene layers for vacuum nanoelectronics. Graphene layers enabled electric con- nection with the graphene layers without postfabrication. The maximum emitted

  2. An Evolutionarily Conserved Enhancer Regulates Bmp4 Expression in Developing Incisor and Limb Bud

    E-Print Network [OSTI]

    Jumlongras, Dolrudee

    To elucidate the transcriptional regulation of Bmp4 expression during organogenesis, we used phylogenetic footprinting and transgenic reporter analyses to identify Bmp4 cis-regulatory modules (CRMs). These analyses identified ...

  3. Endogenous control of stochastic gene expression in the development of Caenorhabditis elegans

    E-Print Network [OSTI]

    Ji, Ni, Ph. D. Massachusetts Institute of Technology

    2013-01-01T23:59:59.000Z

    Studies in the past decade have established gene expression as an inherently variable process. Accompanying this exciting finding is a fundamental question: how do physiological events, such as cell fate specification, ...

  4. How community is expressed in place : spatial manifestations of two parishes

    E-Print Network [OSTI]

    Macy, Christine

    1985-01-01T23:59:59.000Z

    I believe that we become human only through contact with one another, and I am interested in our architectural expression and accommodation of our life in communities specifically communities larger than the family and ...

  5. Acknowledgments The Efficiency Committee expresses its gratitude to Tim Tutt and John Wilson,

    E-Print Network [OSTI]

    #12;#12;Acknowledgments The Efficiency Committee expresses its gratitude to Tim Tutt and John to acknowledge the contributions of the Project Advisory Committee which included representatives of the Investor

  6. Gene Expression and Association Analyses of Stress Responses in Loblolly Pine (Pinus taeda L.)

    E-Print Network [OSTI]

    Seeve, Candace Marie

    2012-02-14T23:59:59.000Z

    The molecular mechanisms underlying disease-resistance and drought-resistance in forest trees are not well understood. Linking variation in gene expression with genetic polymorphisms and with variations in disease- and drought-resistance phenotypes...

  7. Dicer deletion and short RNA expression analysis in mouse embryonic stem cells

    E-Print Network [OSTI]

    Calabrese, Joseph Mauro

    2008-01-01T23:59:59.000Z

    RNA interference (RNAi) manages many aspects of eukaryotic gene expression through sequence-specific interactions with RNA. Short RNAs, 20-30 nucleotides in length, guide the various effector proteins of RNAi to silence ...

  8. E-Print Network 3.0 - auxin-induced wus expression Sample Search...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    results for: auxin-induced wus expression Page: << < 1 2 3 4 5 > >> 1 GENETICS AND GENOMICS WUS and STM-based reporter genes for studying meristem Summary: with the promoter of...

  9. Analysis of genomic Regions of IncreaseD Gene Expression (RIDGE)s in immune activation 

    E-Print Network [OSTI]

    Hansson, Lena

    2009-01-01T23:59:59.000Z

    A RIDGE (Region of IncreaseD Gene Expression), as defined by previous studies, is a consecutive set of active genes on a chromosome that span a region around 110 kbp long. This study investigated RIDGE formation by ...

  10. Genomic studies of motif enrichment and conservation in the regulation of gene expression in the brain

    E-Print Network [OSTI]

    Harmin, David Alan, 1954-

    2008-01-01T23:59:59.000Z

    Several bioinformatic tools will be brought to bear in this thesis to identify specific genomic loci that serve as regulatory gateways of gene expression in brain. These "motifs" are short nucleotide patterns that occur ...

  11. Computational inference of transcriptional regulatory networks from expression proling and transcription

    E-Print Network [OSTI]

    Babu, M. Madan

    Bioinformatics Program, 2 Department of Biology and 3 Biomedical Engineering Department, Boston University, 44Computational inference of transcriptional regulatory networks from expression pro ABSTRACT We have developed a computational method for transcriptional regulatory network inference, CARRIE

  12. Enhancement of Glucocorticoid Receptor-Mediated Gene Expression by Constitutively Active Heat

    E-Print Network [OSTI]

    Abraham, Nader G.

    of a doxycycline (DOX)-inducible vec- tor. In mouse L929 cells, DOX-induced expression of hHSF1-E189 correlated at reduced levels. To as- sess the involvement of hHSF1-E189 in GR activity, DOX-induced expression of hHSF1 ex- pression in these cells was maximal at 24 h of DOX and remained constant up to 72 h. hHSF1-E189

  13. Effects of dietary fiber and carcinogen on fatty acid binding protein expression in exfoliated colonocytes

    E-Print Network [OSTI]

    Clark, Amy Eunice

    1997-01-01T23:59:59.000Z

    EFFECTS OF DIETARY FIBER AND CARCINOGEN ON FATTY ACID BINDING PROTEIN EXPRESSION IN EXFOLIATED COLONOCYTES A Thesis by AMY EUNICE CLARK Submitted to the Office of Gmduate Studies of Texas A&M University in partial fulfillment...) Bryan H. Johnson (Head of Department) August 1997 Major Subject: Nutrition ABSTRACT Effects of Dietary Fiber and Carcinogen on Fatty Acid Binding Protein Expression in Exfoliated Colonocytes. (August 1997) Amy Eunice Clark, B. S. , Texas A...

  14. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer

    SciTech Connect (OSTI)

    Windus, Louisa C.E.; Kiss, Debra L.; Glover, Tristan [Eskitis Institute for Cell and Molecular Therapies, Discovery Biology, Griffith University, Nathan 4111, Brisbane, Queensland (Australia)] [Eskitis Institute for Cell and Molecular Therapies, Discovery Biology, Griffith University, Nathan 4111, Brisbane, Queensland (Australia); Avery, Vicky M., E-mail: v.avery@griffith.edu.au [Eskitis Institute for Cell and Molecular Therapies, Discovery Biology, Griffith University, Nathan 4111, Brisbane, Queensland (Australia)

    2012-11-15T23:59:59.000Z

    Here we report that Prostate Cancer (PCa) cell-lines DU145, PC3, LNCaP and RWPE-1 grown in 3D matrices in contrast to conventional 2D monolayers, display distinct differences in cell morphology, proliferation and expression of important biomarker proteins associated with cancer progression. Consistent with in vivo growth rates, in 3D cultures, all PCa cell-lines were found to proliferate at significantly lower rates in comparison to their 2D counterparts. Moreover, when grown in a 3D matrix, metastatic PC3 cell-lines were found to mimic more precisely protein expression patterns of metastatic tumour formation as found in vivo. In comparison to the prostate epithelial cell-line RWPE-1, metastatic PC3 cell-lines exhibited a down-regulation of E-cadherin and {alpha}6 integrin expression and an up-regulation of N-cadherin, Vimentin and {beta}1 integrin expression and re-expressed non-transcriptionally active AR. In comparison to the non-invasive LNCaP cell-lines, PC3 cells were found to have an up-regulation of chemokine receptor CXCR4, consistent with a metastatic phenotype. In 2D cultures, there was little distinction in protein expression between metastatic, non-invasive and epithelial cells. These results suggest that 3D cultures are more representative of in vivo morphology and may serve as a more biologically relevant model in the drug discovery pipeline. -- Highlights: Black-Right-Pointing-Pointer We developed and optimised 3D culturing techniques for Prostate Cancer cell-lines. Black-Right-Pointing-Pointer We investigated biomarker expression in 2D versus 3D culture techniques. Black-Right-Pointing-Pointer Metastatic PC3 cells re-expressed non-transcriptionally active androgen receptor. Black-Right-Pointing-Pointer Metastatic PCa cell lines retain in vivo-like antigenic profiles in 3D cultures.

  15. Integrins affecting mechanical strain-induced expression of egr-1 in vascular smooth muscle cells 

    E-Print Network [OSTI]

    Lobo, Alan Francis

    2013-02-22T23:59:59.000Z

    Integrins Affecting Mechanical Strain-induced Expression of Egr-I in Vascular Smooth Muscle Cells by Alan' Francis Lobo Submitted to the Office of Honors Programs and Academic Scholarships, Texas ASSAM University in partial fulfillment... Scholarships Attract Integrlns Affecting Mechanical Strain-induced Expression of Egr-I in Vascular Smooth Muscle Cells. Alan Francis Lobo, (Dr. Emily Wilson) University Undergraduate Research Fellow, 1998-1999 Texas Atilt M University Department...

  16. Radiation-Induced Micro-RNA Expression Changes in Peripheral Blood Cells of Radiotherapy Patients

    SciTech Connect (OSTI)

    Templin, Thomas; Paul, Sunirmal; Amundson, Sally A.; Young, Erik F. [Center for Radiological Research, Department of Radiation Oncology, Columbia University Medical Center, New York, NY (United States); Barker, Christopher A.; Wolden, Suzanne L. [Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY (United States); Smilenov, Lubomir B., E-mail: lbs5@columbia.ed [Center for Radiological Research, Department of Radiation Oncology, Columbia University Medical Center, New York, NY (United States)

    2011-06-01T23:59:59.000Z

    Purpose: MicroRNAs (miRNAs), a class of noncoding small RNAs that regulate gene expression, are involved in numerous physiologic processes in normal and malignant cells. Our in vivo study measured miRNA and gene expression changes in human blood cells in response to ionizing radiation, to develop miRNA signatures that can be used as biomarkers for radiation exposure. Methods and Materials: Blood from 8 radiotherapy patients in complete remission 1 or 2 was collected immediately before and 4 hours after total body irradiation with 1.25 Gy x-rays. Both miRNA and gene expression changes were measured by means of quantitative polymerase chain reaction and microarray hybridization, respectively. Hierarchic clustering, multidimensional scaling, class prediction, and gene ontology analysis were performed to investigate the potential of miRNAs to serve as radiation biomarkers and to elucidate their likely physiologic roles in the radiation response. Results: The expression levels of 45 miRNAs were statistically significantly upregulated 4 hours after irradiation with 1.25 Gy x-rays, 27 of them in every patient. Nonirradiated and irradiated samples form separate clusters in hierarchic clustering and multidimensional scaling. Out of 223 differentially expressed genes, 37 were both downregulated and predicted targets of the upregulated miRNAs. Paired and unpaired miRNA-based classifiers that we developed can predict the class membership of a sample with unknown irradiation status, with accuracies of 100% when all 45 upregulated miRNAs are included. Both miRNA control of and gene involvement in biologic processes such as hemopoiesis and the immune response are increased after irradiation, whereas metabolic processes are underrepresented among all differentially expressed genes and the genes controlled by miRNAs. Conclusions: Exposure to ionizing radiation leads to the upregulation of the expression of a considerable proportion of the human miRNAome of peripheral blood cells. These miRNA expression signatures can be used as biomarkers of radiation exposure.

  17. Bayesian Semiparametric Models for Heterogeneous Cross-platform Differential Gene Expression

    E-Print Network [OSTI]

    Dhavala, Soma Sekhar

    2012-02-14T23:59:59.000Z

    BAYESIAN SEMIPARAMETRIC MODELS FOR HETEROGENEOUS CROSS-PLATFORM DIFFERENTIAL GENE EXPRESSION A Dissertation by SOMA SEKHAR DHAVALA Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements... for the degree of DOCTOR OF PHILOSOPHY December 2010 Major Subject: Statistics BAYESIAN SEMIPARAMETRIC MODELS FOR HETEROGENEOUS CROSS-PLATFORM DIFFERENTIAL GENE EXPRESSION A Dissertation by SOMA SEKHAR DHAVALA Submitted to the Office of Graduate Studies...

  18. Knockdown of p53 suppresses Nanog expression in embryonic stem cells

    SciTech Connect (OSTI)

    Abdelalim, Essam Mohamed, E-mail: emohamed@qf.org.qa [Qatar Biomedical Research Institute, Qatar Foundation, Doha 5825 (Qatar); Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan); Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia (Egypt); Tooyama, Ikuo [Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan)] [Molecular Neuroscience Research Center, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192 (Japan)

    2014-01-10T23:59:59.000Z

    Highlights: •We investigate the role of p53 in ESCs in the absence of DNA damage. •p53 knockdown suppresses ESC proliferation. •p53 knockdown downregulates Nanog expression. •p53 is essential for mouse ESC self-renewal. -- Abstract: Mouse embryonic stem cells (ESCs) express high levels of cytoplasmic p53. Exposure of mouse ESCs to DNA damage leads to activation of p53, inducing Nanog suppression. In contrast to earlier studies, we recently reported that chemical inhibition of p53 suppresses ESC proliferation. Here, we confirm that p53 signaling is involved in the maintenance of mouse ESC self-renewal. RNA interference-mediated knockdown of p53 induced downregulation of p21 and defects in ESC proliferation. Furthermore, p53 knockdown resulted in a significant downregulation in Nanog expression at 24 and 48 h post-transfection. p53 knockdown also caused a reduction in Oct4 expression at 48 h post-transfection. Conversely, exposure of ESCs to DNA damage caused a higher reduction of Nanog expression in control siRNA-treated cells than in p53 siRNA-treated cells. These data show that in the absence of DNA damage, p53 is required for the maintenance of mouse ESC self-renewal by regulating Nanog expression.

  19. Expression of PRL proteins at invasive margin of rectal cancers in relation to preoperative radiotherapy

    SciTech Connect (OSTI)

    Wallin, Asa R. [Department of Oncology, Institute of Biomedicine and Surgery, University of Linkoeping, Linkoeping (Sweden); Svanvik, Joar [Department of Surgery, Institute of Biomedicine and Surgery, University of Linkoeping, Linkoeping (Sweden); Adell, Gunnar [Department of Oncology, Institute of Biomedicine and Surgery, University of Linkoeping, Linkoeping (Sweden); Sun Xiaofeng [Department of Oncology, Institute of Biomedicine and Surgery, University of Linkoeping, Linkoeping (Sweden)]. E-mail: xiasu@ibk.liu.se

    2006-06-01T23:59:59.000Z

    Purpose: PRL-3 (phosphatase of regenerating liver) is involved in metastasis of colorectal cancer; however, its therapeutic implication in cancer patients has not been studied. We investigated the relationships of PRL expression to radiotherapy (RT) in rectal cancer patients. Methods and Materials: Phosphatase of regenerating liver expression was immunohistochemically examined in distant (n = 36) and adjacent (n = 82) normal mucosa, primary tumor (n = 125), biopsy specimens (n = 96), and lymph node metastasis (n = 30) from rectal cancer patients participating in a clinical trial of preoperative RT. Results: Phosphatase of regenerating liver expression was increased from the distant to adjacent mucosa and to the primary tumor (p < 0.05). PRL was highly expressed at the invasive margin in 28% of the primary tumors and 26% of the metastases. In the RT group, strong PRL expression at the invasive margin was related to distant recurrence (p 0.006) and poor survival (p = 0.01), but not in the non-RT group. The survival significance remained even after adjusting for Dukes' stage and differentiation (p = 0.02). Additional multivariate analyses showed that the correlation with prognostic significance of PRL differed between the RT and non-RT groups (p = 0.01). Conclusion: Phosphatase of regenerating liver expression (rather than PRL-3 alone) at the invasive margin predicted resistance to RT and unfavorable survival in rectal cancer patients with preoperative RT.

  20. Gene expression of cyclin-dependent kinase inhibitors and effect of heparin on their expression in mice with hypoxia-induced pulmonary hypertension

    SciTech Connect (OSTI)

    Yu Lunyin [Department of Medicine, Pulmonary and Critical Care Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 (United States); Quinn, Deborah A. [Department of Medicine, Pulmonary and Critical Care Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 (United States); Garg, Hari G. [Department of Medicine, Pulmonary and Critical Care Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 (United States); Hales, Charles A. [Department of Medicine, Pulmonary and Critical Care Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 (United States)]. E-mail: chales@partners.org

    2006-07-14T23:59:59.000Z

    The balance between cell proliferation and cell quiescence is regulated delicately by a variety of mediators, in which cyclin-dependent kinases (CDK) and CDK inhibitors (CDKI) play a very important role. Heparin which inhibits pulmonary artery smooth muscle cell (PASMC) proliferation increases the levels of two CDKIs, p21 and p27, although only p27 is important in inhibition of PASMC growth in vitro and in vivo. In the present study we investigated the expression profile of all the cell cycle regulating genes, including all seven CDKIs (p21, p27, p57, p15, p16, p18, and p19), in the lungs of mice with hypoxia-induced pulmonary hypertension. A cell cycle pathway specific gene microarray was used to profile the 96 genes involved in cell cycle regulation. We also observed the effect of heparin on gene expression. We found that (a) hypoxic exposure for two weeks significantly inhibited p27 expression and stimulated p18 activity, showing a 98% decrease in p27 and 81% increase in p18; (b) other CDKIs, p21, p57, p15, p16, and p19 were not affected significantly in response to hypoxia; (c) heparin treatment restored p27 expression, but did not influence p18; (d) ERK1/2 and p38 were mediators in heparin upregulation of p27. This study provides an expression profile of cell cycle regulating genes under hypoxia in mice with hypoxia-induced pulmonary hypertension and strengthens the previous finding that p27 is the only CDKI involved in heparin regulation of PASMC proliferation and hypoxia-induced pulmonary hypertension.

  1. Rev-erb beta regulates the Srebp-1c promoter and mRNA expression in skeletal muscle cells

    SciTech Connect (OSTI)

    Ramakrishnan, Sathiya N.; Lau, Patrick; Crowther, Lisa M. [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia)] [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia); Cleasby, Mark E. [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent's Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia)] [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent's Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia); Millard, Susan; Leong, Gary M. [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia)] [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia); Cooney, Gregory J. [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent's Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia)] [Diabetes and Obesity Research Program, Garvan Institute of Medical Research, St. Vincent's Hospital, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010 (Australia); Muscat, George E.O., E-mail: g.muscat@imb.uq.edu.au [The University of Queensland, Institute for Molecular Bioscience, St. Lucia, Qld 4072 (Australia)

    2009-10-30T23:59:59.000Z

    The nuclear hormone receptor, Rev-erb beta operates as a transcriptional silencer. We previously demonstrated that exogenous expression of Rev-erb{beta}{Delta}E in skeletal muscle cells increased Srebp-1c mRNA expression. We validated these in vitro observations by injection of an expression vector driving Rev-erb{beta}{Delta}E expression into mouse tibialis muscle that resulted in increased Srebp-1c mRNA expression. Paradoxically, Rev-erb{beta} siRNA expression in skeletal muscle cells repressed Srebp-1c expression, and indicated that Rev-erb{beta} expression was necessary for Srebp-1c expression. ChIP analysis demonstrated that Rev-erb{beta} was recruited to the Srebp-1c promoter. Moreover, Rev-erb{beta} trans-activated the Srebp-1c promoter, in contrast, Rev-erb{beta} efficiently repressed the Rev-erb{alpha} promoter, a previously characterized target gene. Finally, treatment with the Rev-erb agonist (hemin) (i) increased the trans-activation of the Srebp-1c promoter by Rev-erb{beta}; and (ii) increased Rev-erb{beta} and Srebp-1c mRNA expression. These data suggest that Rev-erb{beta} has the potential to activate gene expression, and is a positive regulator of Srebp-1c, a regulator of lipogenesis.

  2. Mitochondrial DNA Variants Mediate Energy Production and Expression Levels for CFH, C3 and EFEMP1 Genes: Implications for Age-Related Macular Degeneration

    E-Print Network [OSTI]

    2013-01-01T23:59:59.000Z

    DNA Variants Mediate Energy Production and Expression Levelsmediate not only energy production and cell growth, but alsoDNA Variants Mediate Energy Production and Expression Levels

  3. Can the Analysis of ERCC1 Expression Contribute to Individualized Therapy in Nasopharyngeal Carcinoma?

    SciTech Connect (OSTI)

    Chan, Siu Hong, E-mail: chansh2@ha.org.h [Department of Clinical Oncology, Pamela Youde Nethersole Eastern Hospital (Hong Kong); Cheung, Florence M.F. [Department of Anatomical Pathology, Pamela Youde Nethersole Eastern Hospital (Hong Kong); Ng, Wai Tong [Department of Clinical Oncology, Pamela Youde Nethersole Eastern Hospital (Hong Kong); Choi, Cheuk Wai [School of Public Health, Li Ka Shing Faculty of Medicine, University of Hong Kong (Hong Kong); Cheung, Kin Nam; Yiu, Kwan Ho [Department of Anatomical Pathology, Pamela Youde Nethersole Eastern Hospital (Hong Kong); Lee, Anne W.M. [Department of Clinical Oncology, Pamela Youde Nethersole Eastern Hospital (Hong Kong)

    2011-04-01T23:59:59.000Z

    Purpose: To analyze the expression of excision repair cross-complementation group 1 (ERCC1) protein in predicting the clinical outcome of nasopharyngeal carcinoma (NPC). Methods and Materials: The histologic specimens of 258 patients with Stage III to IVB nonkeratinizing NPC who were treated with radiotherapy alone (Group I) or concurrent-adjuvant chemoradiotherapy (Group II) were retrieved. Immunostaining on ERCC1 protein was performed. The relationship of ERCC1 expression and clinical outcomes was analyzed. Results: The median ERCC1 score (proportion score of positively stained cells times intensity) was 200 (range, 0-300), and ERCC1 expression was defined as high if the score was above the median. In Group I high-score tumor had a statistically lower locoregional failure-free rate (LRFFR) compared with low-score tumor (p < 0.05) but not distant failure-free rate (DFFR) and overall survival (OS). In Group II no statistically differences were noted in LRFFR, DFFR and OS with regard to the ERCC1 expression. Resistance to cisplatin-containing chemotherapy in high-ERCC1 score tumor was not observed in Group II. Interestingly, low-score tumor in Group I achieved similar local and distant control compared with Group II. Multivariate analysis showed that ERCC1 score was an independent prognostic factor in LRFFR (p < 0.05) and approached statistical significance in failure-free survival (p = 0.08) and OS (p = 0.07). Tumor with high ERCC1 score had a 2-fold (95% confidence interval, 1.02-3.85) increased risk of locoregional failure. This may imply an association of ERCC1 expression with the repair of radiation damage. Conclusions: High ERCC1 expression predicts poor locoregional control in NPC. Chemotherapy response is not affected by ERCC1 expression. Further validation is required.

  4. Aryl?hydrocarbon receptor activity modulates prolactin expression in the pituitary

    SciTech Connect (OSTI)

    Moran, Tyler B.; Brannick, Katherine E.; Raetzman, Lori T., E-mail: raetzman@life.illinois.edu

    2012-11-15T23:59:59.000Z

    Pituitary tumors account for 15% of intracranial neoplasms, however the extent to which environmental toxicants contribute to the proliferation and hormone expression of pituitary cells is unknown. Aryl-hydrocarbon receptor (AhR) interacting protein (AIP) loss of function mutations cause somatotrope and lactotrope adenomas in humans. AIP sequesters AhR and inhibits its transcriptional function. Because of the link between AIP and pituitary tumors, we hypothesize that exposure to dioxins, potent exogenous ligands for AhR that are persistent in the environment, may predispose to pituitary dysfunction through activation of AhR. In the present study, we examined the effect of AhR activation on proliferation and endogenous pituitary hormone expression in the GH3 rat somatolactotrope tumor cell line and the effect of loss of AhR action in knockout mice. GH3 cells respond to nM doses of the reversible AhR agonist ?-naphthoflavone with a robust induction of Cyp1a1. Although mRNA levels of the anti-proliferative signaling cytokine TGFbeta1 are suppressed upon ?-naphthoflavone treatment, we did not observe an alteration in cell proliferation. AhR activation with ?-naphthoflavone suppresses Ahr expression and impairs expression of prolactin (PRL), but not growth hormone (GH) mRNA in GH3 cells. In mice, loss of Ahr similarly leads to a reduction in Prl mRNA at P3, while Gh is unaffected. Additionally, there is a significant reduction in pituitary hormones Lhb and Fshb in the absence of Ahr. Overall, these results demonstrate that AhR is important for pituitary hormone expression and suggest that environmental dioxins can exert endocrine disrupting effects at the pituitary. -- Highlights: ? AhR signaling suppresses Prl mRNA expression. ? AhR signaling does not influence pituitary proliferation in culture. ? AhR is necessary for Prl, Lhb and Fshb expression at postnatal day 3.

  5. Investigating the Correspondence Between Transcriptomic and Proteomic Expression Profiles Using Coupled Cluster Models.

    SciTech Connect (OSTI)

    Rogers, Simon; Girolami, Mark; Kolch, Walter; Waters, Katrina M.; Liu, Tao; Thrall, Brian D.; Wiley, H. S.

    2008-12-01T23:59:59.000Z

    Modern transcriptomics and proteomics enable us to survey the expression of RNAs and proteins at large scales. While these data are usually generated and analysed separately, there is an increasing interest in comparing and co-analysing transcriptome and proteome expression data. A major open question is whether transcriptome and proteome expression is linked and how it is coordinated. Results: Here we have developed a probabilistic clustering model that permits analysis of the links between transcriptomic and proteomic profiles in a sensible and flexible manner. Our coupled mixture model defines a prior probability distribution over the component to which a protein profile should be assigned conditioned on which component the associated mRNA profile belongs to. By providing probabilistic assignments this approach sits between the two extremes of concatenating the data on the assumption that mRNA and protein clusters would have a one-to-one relationship, and independent clustering where the mRNA profile provides no information on the protein profile and vice-versa. We apply this approach to a large dataset of quantitative transcriptomic and proteomic expression data obtained from a human breast epithelial cell line (HMEC) stimulated by epidermal growth factor (EGF) over a series of timepoints corresponding to one cell cycle. The results reveal a complex relationship between transcriptome and proteome with most mRNA clusters linked to at least two protein clusters, and vice versa. A more detailed analysis incorporating information on gene function from the gene ontology database shows that a high correlation of mRNA and protein expression is limited to the components of some molecular machines, such as the ribosome, cell adhesion complexes and the TCP-1 chaperonin involved in protein folding. Conclusions: The dynamic regulation of the transcriptome and proteome in mammalian cells in response to an acute mitogenic stimulus appears largely independent with very little correspondence between mRNA and protein expression. The exceptions involve a few selected multi-protein complexes that require the stoichiometric expression of components for correct function. This finding has wide ramifications regarding the understanding of gene and protein expression including its control and evolution. It also shows that transcriptomic and proteomic expression analysis are complementary and non-redundant.

  6. Knowledge-based analysis of microarray gene expression data by using support vector machines

    SciTech Connect (OSTI)

    William Grundy; Manuel Ares, Jr.; David Haussler

    2001-06-18T23:59:59.000Z

    The authors introduce a method of functionally classifying genes by using gene expression data from DNA microarray hybridization experiments. The method is based on the theory of support vector machines (SVMs). SVMs are considered a supervised computer learning method because they exploit prior knowledge of gene function to identify unknown genes of similar function from expression data. SVMs avoid several problems associated with unsupervised clustering methods, such as hierarchical clustering and self-organizing maps. SVMs have many mathematical features that make them attractive for gene expression analysis, including their flexibility in choosing a similarity function, sparseness of solution when dealing with large data sets, the ability to handle large feature spaces, and the ability to identify outliers. They test several SVMs that use different similarity metrics, as well as some other supervised learning methods, and find that the SVMs best identify sets of genes with a common function using expression data. Finally, they use SVMs to predict functional roles for uncharacterized yeast ORFs based on their expression data.

  7. Quantifying extrinsic noise in gene expression using the maximum entropy framework

    E-Print Network [OSTI]

    Purushottam D. Dixit

    2013-04-04T23:59:59.000Z

    We present a maximum entropy framework to separate intrinsic and extrinsic contributions to noisy gene expression solely from the profile of expression. We express the experimentally accessible probability distribution of the copy number of the gene product (mRNA or protein) by accounting for possible variations in extrinsic factors. The distribution of extrinsic factors is estimated using the maximum entropy principle. Our results show that extrinsic factors qualitatively and quantitatively affect the probability distribution of the gene product. We work out, in detail, the transcription of mRNA from a constitutively expressed promoter in {\\it E. coli}. We suggest that the variation in extrinsic factors may account for the observed {\\it wider than Poisson} distribution of mRNA copy numbers. We successfully test our framework on a numerical simulation of a simple gene expression scheme that accounts for the variation in extrinsic factors. We also make falsifiable predictions, some of which are tested on previous experiments in {\\it E. coli} while others need verification. Application of the current framework to more complex situations is also discussed.

  8. Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems

    SciTech Connect (OSTI)

    Conover, David R.; Crawford, Aladsair J.; Viswanathan, Vilayanur V.; Ferreira, Summer; Schoenwald, David

    2014-06-01T23:59:59.000Z

    The Protocol for Uniformly Measuring and Expressing the Performance of Energy Storage Systems (PNNL-22010) was first issued in November 2012 as a first step toward providing a foundational basis for developing an initial standard for the uniform measurement and expression of energy storage system (ESS) performance. Its subsequent use in the field and review by the protocol working group and most importantly the users’ subgroup and the thermal subgroup has led to the fundamental modifications reflected in this update of the 2012 Protocol. As an update of the 2012 Protocol, this document (the June 2014 Protocol) is intended to supersede its predecessor and be used as the basis for measuring and expressing ESS performance. The foreword provides general and specific details about what additions, revisions, and enhancements have been made to the 2012 Protocol and the rationale for them in arriving at the June 2014 Protocol.

  9. Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation

    SciTech Connect (OSTI)

    Reed, J.C.; Tsujimoto, Y.; Alpers, J.D.; Croce, C.M.; Nowell, P.C.

    1987-06-05T23:59:59.000Z

    The bcl-2 and c-myc proto-oncogenes are brought into juxtaposition with the immuno-globulin heavy chain locus in particular B-cell lymphomas, resulting in high levels of constitutive accumulation of their messenger RNAs. Precisely how the products of the bcl-2 and c-myc genes contribute to tumorigenesis is unknown, but observations that c-myc expression is rapidly induced in nonneoplastic lymphocytes upon stimulation of proliferation raise the possibility that this proto-oncogene is involved in the control of normal cellular growth. In addition to c-myc, the bcl-2 proto-oncogene also was expressed in normal human B and T lymphocytes after stimulation with appropriate mitogens. Comparison of the regulation of the expression of these proto-oncogenes demonstrated marked differences and provided evidence that, in contrast to c-myc, levels of bcl-2 messenger RNA are regulated primarily though transcriptional mechanisms. 10 references, 3 figures.

  10. Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages

    DOE Patents [OSTI]

    Studier, F.W.; Dubendorff, J.W.

    1998-11-03T23:59:59.000Z

    This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.

  11. Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages

    DOE Patents [OSTI]

    Studier, F.W.; Dubendorff, J.W.

    1998-10-20T23:59:59.000Z

    This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.

  12. Lumican induces human corneal epithelial cell migration and integrin expression via ERK 1/2 signaling

    SciTech Connect (OSTI)

    Seomun, Young [Laboratory of Ophthalmology and Visual Science, Korean Eye Tissue and Gene Bank related to Blindness, College of Medicine, Catholic University of Korea, 505 Banpo-dong, Seocho-ku, Seoul 137-040 (Korea, Republic of); Joo, Choun-Ki [Laboratory of Ophthalmology and Visual Science, Korean Eye Tissue and Gene Bank related to Blindness, College of Medicine, Catholic University of Korea, 505 Banpo-dong, Seocho-ku, Seoul 137-040 (Korea, Republic of)], E-mail: ckjoo@catholic.ac.kr

    2008-07-18T23:59:59.000Z

    Lumican is a major proteoglycans of the human cornea. Lumican knock-out mice have been shown to lose corneal transparency and to display delayed wound healing. The purpose of this study was to define the role of lumican in corneal epithelial cell migration. Over-expression of lumican in human corneal epithelial (HCE-T) cells increased both cell migration and proliferation, and increased levels of integrins {alpha}2 and {beta}1. ERK 1/2 was also activated in lumican over-expressed cells. When we treated HCE-T cells with the ERK-specific inhibitor U0126, cell migration and the expression of integrin {beta}1 were completely blocked. These data provide evidence that lumican stimulates cell migration in the corneal epithelium by activating ERK 1/2, and point to a novel signaling pathway implicated in corneal epithelial cell migration.

  13. Femtosecond spectroscopy probes the folding quality of antibody fragments expressed as GFP fusions in the cytoplasm

    SciTech Connect (OSTI)

    Didier, P. [Faculte de Pharmacie, UMR 7175, 74, route du Rhin, 67412 Illkirch (France); Weiss, E.; Sibler, A.-P. [Ecole Superieure de Biotechnologie de Strasbourg, UMR 7175, Boulevard Sebastien Brant, F-67412 Illkirch (France); Philibert, P.; Martineau, P. [Centre de recherche en cancerologie de Montpellier, UMR 5160, Val d'Aurelle-Paul Lamarque, 34298 Montpellier cedex 5 (France); Bigot, J.-Y. [Institut de Physique et Chimie des Materiaux de Strasbourg, UMR 7504, 23, rue du Loess, F-67037 Strasbourg (France); Guidoni, L. [Institut de Physique et Chimie des Materiaux de Strasbourg, UMR 7504, 23, rue du Loess, F-67037 Strasbourg (France); Laboratoire Materiaux et Phenomenes Quantiques, UMR 7162, Batiment Condorcet, 10 rue Alice Domon et Leonie Duquet, 75205 Paris cedex 13 (France)], E-mail: luca.guidoni@univ-paris-diderot.fr

    2008-02-22T23:59:59.000Z

    Time-resolved femtosecond spectroscopy can improve the application of green fluorescent proteins (GFPs) as protein-folding reporters. The study of ultrafast excited-state dynamics (ESD) of GFP fused to single chain variable fragment (scFv) antibody fragments, allowed us to define and measure an empirical parameter that only depends on the folding quality (FQ) of the fusion. This method has been applied to the analysis of genetic fusions expressed in the bacterial cytoplasm and allowed us to distinguish folded and thus functional antibody fragments (high FQ) with respect to misfolded antibody fragments. Moreover, these findings were strongly correlated to the behavior of the same scFvs expressed in animal cells. This method is based on the sensitivity of the ESD to the modifications in the tertiary structure of the GFP induced by the aggregation state of the fusion partner. This approach may be applicable to the study of the FQ of polypeptides over-expressed under reducing conditions.

  14. Human Cementum Protein 1 induces expression of bone and cementum proteins by human gingival fibroblasts

    SciTech Connect (OSTI)

    Carmona-Rodriguez, Bruno [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico); Alvarez-Perez, Marco Antonio [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico); Narayanan, A. Sampath [Department of Pathology, School of Medicine, UW, Seattle (United States); Zeichner-David, Margarita [Center for Craniofacial Molecular Biology, School of Dentistry, USC, Los Angeles (United States); Reyes-Gasga, Jose [Instituto de Fisica, UNAM (Mexico); Molina-Guarneros, Juan [Facultad de Medicina, UNAM (Mexico); Garcia-Hernandez, Ana Lilia [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico); Suarez-Franco, Jose Luis [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico); Chavarria, Ivet Gil [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico); Villarreal-Ramirez, Eduardo [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico); Arzate, Higinio [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico)]. E-mail: harzate@servidor.unam.mx

    2007-07-06T23:59:59.000Z

    We recently presented evidence showing that a human cementoblastoma-derived protein, named Cementum Protein 1 (CEMP1) may play a role as a local regulator of cementoblast differentiation and cementum-matrix mineralization. This protein was shown to be expressed by cementoblasts and progenitor cells localized in the periodontal ligament. In this study we demonstrate that transfection of CEMP1 into human gingival fibroblasts (HGF) induces mineralization and expression of bone and cementum-matrix proteins. The transfected HGF cells had higher alkaline phosphatase activity and proliferation rate and they expressed genes for alkaline phosphatase, bone sialoprotein, osteocalcin, osteopontin, the transcription factor Runx2/Cbfa1, and cementum attachment protein (CAP). They also produced biological-type hydroxyapatite. These findings indicate that the CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and that it might have a potential function in cementum and bone formation.

  15. RIKEN IMS Summer Program 2014 http://www.ims.riken.jp/risp/

    E-Print Network [OSTI]

    Fukai, Tomoki

    . Accommodations and travel expenses All participants will stay at the Rembrandt Inn Yokohama Tsurumi of itinerary. (Rembrandt Inn Yokohama Tsurumi: http://inn.rembrandt-hotel.jp/yokohama/e/ ) Cancellation policy

  16. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs

    DOE Patents [OSTI]

    Glazer, Alexander N.; Tooley, Aaron J.; Cai, Yuping

    2004-05-25T23:59:59.000Z

    Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.

  17. Matrigel Basement Membrane Matrix influences expression of microRNAs in cancer cell lines

    SciTech Connect (OSTI)

    Price, Karina J. [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia) [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); School of Medicine and Pharmacology, University of Western Australia, Nedlands, WA 6008 (Australia); Tsykin, Anna [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia) [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia); School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005 (Australia); Giles, Keith M. [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia)] [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); Sladic, Rosemary T. [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia)] [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia); Epis, Michael R. [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia)] [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); Ganss, Ruth [Laboratory for Cancer Medicine Angiogenesis Unit, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia)] [Laboratory for Cancer Medicine Angiogenesis Unit, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); Goodall, Gregory J. [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia) [Centre for Cancer Biology, SA Pathology, Adelaide, SA 5000 (Australia); School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005 (Australia); Department of Medicine, University of Adelaide, Adelaide, SA 5005 (Australia); Leedman, Peter J., E-mail: peter.leedman@waimr.uwa.edu.au [Laboratory for Cancer Medicine, Western Australian Institute for Medical Research and University of Western Australia Centre for Medical Research, Perth, WA 6000 (Australia); School of Medicine and Pharmacology, University of Western Australia, Nedlands, WA 6008 (Australia)

    2012-10-19T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Matrigel alters cancer cell line miRNA expression relative to culture on plastic. Black-Right-Pointing-Pointer Many identified Matrigel-regulated miRNAs are implicated in cancer. Black-Right-Pointing-Pointer miR-1290, -210, -32 and -29b represent a Matrigel-induced miRNA signature. Black-Right-Pointing-Pointer miR-32 down-regulates Integrin alpha 5 (ITGA5) mRNA. -- Abstract: Matrigel is a medium rich in extracellular matrix (ECM) components used for three-dimensional cell culture and is known to alter cellular phenotypes and gene expression. microRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have roles in cancer. While miRNA profiles of numerous cell lines cultured on plastic have been reported, the influence of Matrigel-based culture on cancer cell miRNA expression is largely unknown. This study investigated the influence of Matrigel on the expression of miRNAs that might facilitate ECM-associated cancer cell growth. We performed miRNA profiling by microarray using two colon cancer cell lines (SW480 and SW620), identifying significant differential expression of miRNAs between cells cultured in Matrigel and on plastic. Many of these miRNAs have previously been implicated in cancer-related processes. A common Matrigel-induced miRNA signature comprised of up-regulated miR-1290 and miR-210 and down-regulated miR-29b and miR-32 was identified using RT-qPCR across five epithelial cancer cell lines (SW480, SW620, HT-29, A549 and MDA-MB-231). Experimental modulation of these miRNAs altered expression of their known target mRNAs involved in cell adhesion, proliferation and invasion, in colon cancer cell lines. Furthermore, ITGA5 was identified as a novel putative target of miR-32 that may facilitate cancer cell interactions with the ECM. We propose that culture of cancer cell lines in Matrigel more accurately recapitulates miRNA expression and function in cancer than culture on plastic and thus is a valuable approach to the in vitro study of miRNAs.

  18. Empirical Study of the Healing Nature of Artistic Expression: Using Mandalas with the Positive Emotions of Love and Joy

    E-Print Network [OSTI]

    Henderson, Patti Gail

    2012-07-16T23:59:59.000Z

    EMPIRICAL STUDY OF THE HEALING NATURE OF ARTISTIC EXPRESSION: USING MANDALAS WITH THE POSITIVE EMOTIONS OF LOVE AND JOY A Dissertation by PATTI GAIL HENDERSON Submitted to the Office of Graduate Studies of Texas... Empirical Study of the Healing Nature of Artistic Expression: Using Mandalas with the Positive Emotions of Love and Joy Copyright 2012 Patti Gail Henderson EMPIRICAL STUDY OF THE HEALING NATURE OF ARTISTIC EXPRESSION: USING MANDALAS...

  19. Classification of Rice (Oryza sativa L. japonica Nipponbare) Immunophilins (FKBPs, CYPs) and Expression Patterns under Water Stress

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    during exposure to heat stress, whereas it was localized inexpression was induced by heat stress and developmentallyin the nucleus during heat stress [19]. The expression of

  20. Production of glycoprotein-deleted rabies viruses for monosynaptic tracing and high-level gene expression in neurons

    E-Print Network [OSTI]

    Wickersham, Ian R.

    Recombinant rabies viruses rendered replication-deficient by the deletion of their envelope glycoprotein gene are useful tools for neuroscientists, permitting (1) extraordinarily high transgene expression levels within ...

  1. Effects of interferon-[] on activation of the Jak/Stat signal transduction pathway and regulation of ovine endometrial gene expression

    E-Print Network [OSTI]

    Stewart, Milton David

    2001-01-01T23:59:59.000Z

    GE) on Days 14 and 15 of the estrous cycle (Silvia and Raw, 1993; Charpigny et al. , 1997; Gray et al. , 2000). The sGE is the GE within the stratum compactum of the endometrium that is phenotypically identical to the LE. Pulsatile secretion of PGF.../or IRFs to suppress ERa and OTR expression in the LE and sGE. Although IFN~ suppresses ERa and OTR expression in the LE and sGE, it does not induce ISG expression in these cell types (Choi et al. , 2001). Instead, IFN~ induces ISG expression solely...

  2. Expression of Multiple Artificial MicroRNAs from a Chicken miRNA126-Based Lentiviral Vector

    E-Print Network [OSTI]

    Chen, Steve C.-Y.

    Background: The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the

  3. The epistle dedicatory in English drama: 1698-1730

    E-Print Network [OSTI]

    McConal, Shirley Jean

    1974-01-01T23:59:59.000Z

    are frequently 7 acted in the Play-houses in Drury-lane and Lincoln's-Inn-Fields in this Country are full of prophane, irreverent, lewd, indecent, and immoral expressions, and tend to the displeasure of Almighty God, and to the corruption of the auditory both... ministers and Members of Parliament as writers were sought after and wooed. Beljame lists many offices given to playwrights during these political years: Charles Shadwell Poet Laureate Historiographer Royal Nicholas Rowe Poet Laureate Land Surveyor...

  4. NANO EXPRESS Open Access Ultrafast nano-oscillators based on interlayer-

    E-Print Network [OSTI]

    Li, Teng

    NANO EXPRESS Open Access Ultrafast nano-oscillators based on interlayer- bridged carbon nanoscrolls nano-oscillators based on carbon nanoscrolls (CNSs) using molecular dynamics simulations. Initiated of gigahertz. We demonstrate an effective strategy to reduce the dissipation of the CNS-based nano

  5. Kinetic and Pharmacological Properties of GABAA Receptors in Single Thalamic Neurons and GABAA Subunit Expression

    E-Print Network [OSTI]

    Huguenard, John R.

    Kinetic and Pharmacological Properties of GABAA Receptors in Single Thalamic Neurons and GABAA Subunit Expression S. H. BROWNE,1, * J. KANG,2, * G. AKK,2 L. W. CHIANG,1 H. SCHULMAN,1 J. R. HUGUENARD,2, and D. A. Prince. Kinetic and pharmacological prop- erties of GABAA receptors in single thalamic neurons

  6. EIS-0447: Champlain Hudson Power Express Transmission Line Project, New York

    Broader source: Energy.gov [DOE]

    This EIS evaluated the potential environmental impacts of a DOE proposal to grant a Presidential permit to Champlain Hudson Power Express, Inc., to construct, operate, maintain, and connect a new 1000-megawatt (MW) electric transmission system across the U.S.-Canada border in northeastern New York State. The proposed transmission line would run from the Canadian Province of Quebec to New York City.

  7. Perinuclear Mlp proteins downregulate gene expression in response to a defect in mRNA export

    E-Print Network [OSTI]

    Bedwell, David M.

    Perinuclear Mlp proteins downregulate gene expression in response to a defect in mRNA export of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland The mRNA export adaptor Yra1p/REF contributes to nas- cent mRNP assembly and recruitment of the export recep- tor Mex67p. yra1 mutants exhibit m

  8. Victoria Arango, PhD NEURONAL TRYPTOPHAN HYDROXYLASE mRNA EXPRESSION IN THE HUMAN

    E-Print Network [OSTI]

    Sibille, Etienne

    -submitted to Neuropsychopharmacology, August 4, 2005 Running Title: TPH2 mRNA in depressed suicides #12;Victoria Arango, PhD 2 ABSTRACTVictoria Arango, PhD 1 NEURONAL TRYPTOPHAN HYDROXYLASE mRNA EXPRESSION IN THE HUMAN DORSAL. John Mann, MD 1,2 Victoria Arango, PhD 1,2 1 Department of Neuroscience, New York State Psychiatric

  9. Genetic Background Modulates Gene Expression Profile Induced by Skin Irradiation in Ptch1 Mice

    SciTech Connect (OSTI)

    Galvan, Antonella; Noci, Sara [Department of Experimental Oncology and Laboratories, Fondazione IRCCS Istituto Nazionale Tumori, Milan (Italy); Mancuso, Mariateresa; Pazzaglia, Simonetta; Saran, Anna [ENEA Laboratories, Rome (Italy); Dragani, Tommaso A. [Department of Experimental Oncology and Laboratories, Fondazione IRCCS Istituto Nazionale Tumori, Milan (Italy)], E-mail: tommaso.dragani@istitutotumori.mi.it

    2008-12-01T23:59:59.000Z

    Purpose: Ptch1 germ-line mutations in mice predispose to radiation-induced basal cell carcinoma of the skin, with tumor incidence modulated by the genetic background. Here, we examined the possible mechanisms underlying skin response to radiation in F1 progeny of Ptch1{sup neo67/+} mice crossed with either skin tumor-susceptible (Car-S) or -resistant (Car-R) mice and X-irradiated (3 Gy) at 2 days of age or left untreated. Methods and Materials: We conducted a gene expression profile analysis in mRNA samples extracted from the skin of irradiated or control mice, using Affymetrix whole mouse genome expression array. Confirmation of the results was done using real-time reverse-transcriptase polymerase chain reaction. Results: Analysis of the gene expression profile of normal skin of F1 mice at 4 weeks of age revealed a similar basal profile in the nonirradiated mice, but alterations in levels of 71 transcripts in irradiated Ptch1{sup neo67/+} mice of the Car-R cross and modulation of only eight genes in irradiated Ptch1{sup neo67/+} mice of the Car-S cross. Conclusions: These results indicate that neonatal irradiation causes a persistent change in the gene expression profile of the skin. The tendency of mice genetically resistant to skin tumorigenesis to show a more complex pattern of transcriptional response to radiation than do genetically susceptible mice suggests a role for this response in genetic resistance to basal cell tumorigenesis.

  10. NANO EXPRESS Open Access Au/Pd core-shell nanoparticles with varied hollow

    E-Print Network [OSTI]

    Liu, Fuqiang

    NANO EXPRESS Open Access Au/Pd core-shell nanoparticles with varied hollow Au cores for enhanced has been developed to synthesize Au/Pd core-shell nanoparticles via galvanic replacement of Cu by Pd on hollow Au nanospheres. The unique nanoparticles were characterized by X-ray diffraction, X

  11. ORIGINAL ARTICLE Low or absent SPARC expression in acute myeloid leukemia with MLL rearrangements

    E-Print Network [OSTI]

    Ford, James

    ORIGINAL ARTICLE Low or absent SPARC expression in acute myeloid leukemia with MLL rearrangements January 2006 Keywords: matricellular glycoproteins; acute myeloid leukemia; MLL; DNA methylation­10% of acute myeloid leukemia (AML) patients and in some studies confer an increased risk of treatment failure

  12. Gene expression networks underlying retinoic acidinduced differentiation of acute promyelocytic leukemia cells

    E-Print Network [OSTI]

    Tian, Weidong

    Introduction Acute promyelocytic leukemia (APL) is an interesting model in the study of human cancer becauseNEOPLASIA Gene expression networks underlying retinoic acid­induced differentiation of acute promyelocytic leukemia cells Ting-Xi Liu, Ji-Wang Zhang, Jiong Tao, Ruo-Bo Zhang, Qing-Hua Zhang, Chun-Jun Zhao

  13. Expression profiles of acute lymphoblastic and myeloblastic leukemias with ALL-1 rearrangements

    E-Print Network [OSTI]

    Domany, Eytan

    Expression profiles of acute lymphoblastic and myeloblastic leukemias with ALL-1 rearrangements T­10% of acute lymphoblastic leukemias (ALLs) and acute myeloid leukemias (AMLs) by fusion to other genes is a region of recurrent rearrange- ments in human acute leukemias. These rearrangements, usu- ally

  14. A trans-Acting Riboswitch Controls Expression of the Virulence Regulator

    E-Print Network [OSTI]

    Bedwell, David M.

    ; Romby et al., 2006; Toledo- Arana et al., 2007). They act either by sequestering target proteins path- ogenic bacteria (Toledo-Arana et al., 2007). For instance, RNAIII of Staphylococcus aureus has, with at least two being involved in virulence (Toledo-Arana et al., 2009). Expression of the virulence regulator

  15. New Insights Into the Role of Equine Infectious Anemia Virus S2 Protein in Disease Expression

    E-Print Network [OSTI]

    Covaleda Salas, Lina M.

    2011-08-08T23:59:59.000Z

    macrophages. Specifically, S2 enhances the expression of IL-1 alpha, IL-1 beta IL-8, MCP-2, MIP-1 beta, IP-10 and a newly discovered cytokine, IL-34. Involvement of S2 in cytokine and chemokine dysregulation may contribute to disease development by optimizing...

  16. Identification and Characterization of Bovine Pol III Promoters to Express a Short-Hairpin RNA

    E-Print Network [OSTI]

    Peoples, Michael D 1978-

    2010-12-08T23:59:59.000Z

    ). Each bPol III promoter was able to express a functional shRNA resulting in a reduction of luciferase activity greater than 68 percent. The bH1 and bU6-2 Luc shRNA vectors were the most effective vectors when transfected with >76 percent (p-value <0...

  17. Rapid Communication Herpes simplex virus 1 microRNAs expressed abundantly during latent infection are

    E-Print Network [OSTI]

    Knipe, David M.

    Rapid Communication Herpes simplex virus 1 microRNAs expressed abundantly during latent infection Available online 23 July 2011 Keywords: Herpes simplex virus MicroRNAs Latency Gene regulation Several herpes simplex virus 1 microRNAs are encoded within or near the latency associated transcript (LAT) locus

  18. Non-linear Predictors for Facial Feature Tracking Across Pose and Expression

    E-Print Network [OSTI]

    Bowden, Richard

    Non-linear Predictors for Facial Feature Tracking Across Pose and Expression Tim Sheerman Email: t.sheerman-chase,e.ong,r.bowden@surrey.ac.uk Abstract--This paper proposes a non-linear predictor facial feature. Linear regression is only effective when pose changes are limited. As pose variations

  19. Statistics & Gene Expression Data Analysis Note 8: Binary Regression Outcomes and classification probabilities

    E-Print Network [OSTI]

    West, Mike

    classification, validation, prognosis Binary regression models · Linear regression model based on regression ­ Standard statistical models transform from real-value to (0, 1) using a specified non-linear functionStatistics & Gene Expression Data Analysis Note 8: Binary Regression Outcomes and classification

  20. Research article Differential expression of heat shock protein genes in preconditioning

    E-Print Network [OSTI]

    Ramakrishnan, Naren

    Research article Differential expression of heat shock protein genes in preconditioning February 2010 Keywords: GRP94 HSP70 Promoter analysis sHSP a b s t r a c t Heat shock proteins (HSPs) are induced not only under heat stress conditions but also under other environmental stresses such as water

  1. Translational regulations as specic traits of chloroplast gene expression Yves Choquet

    E-Print Network [OSTI]

    that convert light energy into chemical energy sources that fuel plant cell metabolism. Their inner membrane characteristics concur to set the major step in the regulation of chloroplast gene expression at the translational level, leading to a surprisingly low sensitivity of chloroplast protein synthesis in response

  2. Heart valve cardiomyocytes of mouse embryos express the serotonin transporter SERT

    SciTech Connect (OSTI)

    Pavone, Luigi Michele [Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino 1, 80137 Naples (Italy); Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Naples (Italy)], E-mail: pavone@dbbm.unina.it; Spina, Anna; Lo Muto, Roberta; Santoro, Dionea [Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino 1, 80137 Naples (Italy); Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Naples (Italy); Mastellone, Vincenzo [Department of Experimental Medicine 'G. Salvatore', University of Magna Graecia, Catanzaro (Italy); Avallone, Luigi [Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino 1, 80137 Naples (Italy)

    2008-12-12T23:59:59.000Z

    Multiple evidence demonstrate a role for serotonin and its transporter SERT in heart valve development and disease. By utilizing a Cre/loxP system driven by SERT gene expression, we recently demonstrated a regionally restricted distribution of SERT-expressing cells in developing mouse heart. In order to characterize the cell types exhibiting SERT expression within the mouse heart valves at early developmental stages, in this study we performed immunohistochemistry for Islet1 (Isl1) and connexin-43 (Cx-43) on heart sections from SERT{sup Cre/+};ROSA26R embryos previously stained with X-gal. We observed the co-localization of LacZ staining with Isl1 labelling in the outflow tract, the right ventricle and the conal region of E11.5 mouse heart. Cx-43 labelled cells co-localized with LacZ stained cells in the forming atrioventricular valves. These results demonstrate the cardiomyocyte phenotype of SERT-expressing cells in heart valves of the developing mouse heart, thus suggesting an active role of SERT in early heart valve development.

  3. Navy Leadership Disturbed by "Spice" Usage Rise Navy leaders are expressing alarm at recent statistics that

    E-Print Network [OSTI]

    Navy Leadership Disturbed by "Spice" Usage Rise Navy leaders are expressing alarm at recent, mimicking the chemical compounds found in the drug. These products are banned for Navy personnel (NAVADMIN) 108/10 in March 2010, which reemphasized the Navy's drug policy, the U.S. Navy has been

  4. Memory-influencing intra-basolateral amygdala drug infusions modulate expression of Arc

    E-Print Network [OSTI]

    O'Toole, Alice J.

    Memory-influencing intra-basolateral amygdala drug infusions modulate expression of Arc protein processes. Infusions of the -adrenoreceptor agonist, clen- buterol, into the BLA immediately after training increased Arc protein levels in the dorsal hippocampus. Additionally, posttraining intra-BLA infusions

  5. Arsenic-induced alteration in the expression of genes related to type 2 diabetes mellitus

    SciTech Connect (OSTI)

    Diaz-Villasenor, Andrea [Department of Genomic Medicine and Environmental Toxicology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Circuito Escolar, Cd. Universitaria, Coyoacan 04510 Mexico, DF (Mexico)], E-mail: andreadv@biomedicas.unam.mx; Burns, Anna L. [Department of Genomic Medicine and Environmental Toxicology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Circuito Escolar, Cd. Universitaria, Coyoacan 04510 Mexico, DF (Mexico); Facultad de Medicina, Universidad Nacional Autonoma de Mexico (Mexico); Hiriart, Marcia [Department of Biophysics, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico (Mexico); Cebrian, Mariano E. [Seccion Externa de Toxicologia, CINVESTAV, IPN (Mexico); Ostrosky-Wegman, Patricia [Department of Genomic Medicine and Environmental Toxicology, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Circuito Escolar, Cd. Universitaria, Coyoacan 04510 Mexico, DF (Mexico)

    2007-12-01T23:59:59.000Z

    Chronic exposure to high concentrations of arsenic in drinking water is associated with an increased risk for developing type 2 diabetes. The present revision focuses on the effect of arsenic on tissues that participate directly in glucose homeostasis, integrating the most important published information about the impairment of the expression of genes related to type 2 diabetes by arsenic as one of the possible mechanisms by which it leads to the disease. Many factors are involved in the manner in which arsenic contributes to the occurrence of diabetes. The reviewed studies suggest that arsenic might increase the risk for type 2 diabetes via multiple mechanisms, affecting a cluster of regulated events, which in conjunction trigger the disease. Arsenic affects insulin sensitivity in peripheral tissue by modifying the expression of genes involved in insulin resistance and shifting away cells from differentiation to the proliferation pathway. In the liver arsenic disturbs glucose production, whereas in pancreatic beta-cells arsenic decreases insulin synthesis and secretion and reduces the expression of antioxidant enzymes. The consequences of these changes in gene expression include the reduction of insulin secretion, induction of oxidative stress in the pancreas, alteration of gluconeogenesis, abnormal proliferation and differentiation pattern of muscle and adipocytes as well as peripheral insulin resistance.

  6. Regulation of E2F-1 gene expression in human breast cancer cells 

    E-Print Network [OSTI]

    Ngwenya, Sharon Khethiwe

    2005-08-29T23:59:59.000Z

    17?-Estradiol induces E2F-1 gene expression in ZR-75 and MCF-7 human breast cancer cells. Analysis of the E2F-1 gene promoter in MCF-7 cells previously showed that hormone-induced transactivation required interactions between ...

  7. Seasonal Changes in Bacterial and Archaeal Gene Expression Patterns across Salinity Gradients in the

    E-Print Network [OSTI]

    their metabolic activities, microbial populations mediate the impact of high gradient regions on ecologicalSeasonal Changes in Bacterial and Archaeal Gene Expression Patterns across Salinity Gradients in nitrogen and carbon metabolism in the CRCM. Initial experiments with the environmental microarrays were

  8. Acrolein increases 5-lipoxygenase expression in murine macrophages through activation of ERK pathway

    SciTech Connect (OSTI)

    Kim, Chae E.; Lee, Seung J.; Seo, Kyo W.; Park, Hye M.; Yun, Jung W.; Bae, Jin U.; Bae, Sun S.; Kim, Chi D., E-mail: chidkim@pusan.ac.k

    2010-05-15T23:59:59.000Z

    Episodic exposure to acrolein-rich pollutants has been linked to acute myocardial infarction, and 5-lipoxygenase (5-LO) is involved in the production of matrix metalloproteinase-9 (MMP-9), which destabilizes atherosclerotic plaques. Thus, the present study determined the effect of acrolein on 5-LO/leukotriene B{sub 4} (LTB{sub 4}) production in murine macrophages. Stimulation of J774A.1 cells with acrolein led to increased LTB{sub 4} production in association with increased 5-LO expression. Acrolein-evoked 5-LO expression was blocked by pharmacological inhibition of the ERK pathway, but not by inhibitors for JNK and p38 MAPK pathways. In line with these results, acrolein exclusively increased the phosphorylation of ERK among these MAPK, suggesting a role for the ERK pathway in acrolein-induced 5-LO expression with subsequent production of LTB{sub 4}. Among the receptor tyrosine kinases including epidermal growth factor receptor (EGFR) and platelet derived growth factor receptor (PDGFR), acrolein-evoked ERK phosphorylation was attenuated by AG1478, an EGFR inhibitor, but not by AG1295, a PDGFR inhibitor. In addition, acrolein-evoked 5-LO expression was also inhibited by inhibition of EGFR pathway, but not by inhibition of PDGFR pathway. These observations suggest that acrolein has a profound effect on the 5-LO pathway via an EGFR-mediated activation of ERK pathway, leading to acute ischemic syndromes through the generation of LTB{sub 4}, subsequent MMP-9 production and plaque rupture.

  9. ASIAN: a web server for inferring a regulatory network framework from gene expression profiles

    E-Print Network [OSTI]

    Babu, M. Madan

    ASIAN: a web server for inferring a regulatory network framework from gene expression profiles developed to deduce the gene regulatory network. Here, we describe our web server for inferring a framework matrix between variables, and therefore, our server can analyze a wide variety of data within

  10. AESRC 2012, Kingston March 23-25th THE "SURFACE" EXPRESSION: WHAT DO GEOCHEMICAL ANOMALIES IN

    E-Print Network [OSTI]

    IN SURFACE MEDIA AND SHALLOW SANDSTONES OVERLYING THE PHOENIX URANIUM DEPOSIT, ATHABASCA BASIN, SASKATCHEWAN to examine whether surficial geochemical anomalies exist for such a deeply buried uranium deposit. For our expression in surface media provides excellent exploration tools for deeply seated unconformity

  11. OETS SPEAK of attaining some distance from the world --finding some strangeness --to express the

    E-Print Network [OSTI]

    Quigg, Chris

    the truths that make a rock stony or a love sublime. Like the poet, the physicist seeks not merely to name sublime. Like the poet, the physicist seeks not merely to name the properties of nature, but to plumb strangeness -- to express the truths that make a rock stony or a love sublime. Like the poet, the physicist

  12. Hand gestures for medical applica-tions provide an expressive, natural

    E-Print Network [OSTI]

    Wachs, Juan

    members to increasingly interact with medical equipment and computer systems. These advances haveHand gestures for medical applica- tions provide an expressive, natural and intuitive way reviews the highlights of a novel gesture-based system for medical image browsing in the OR called Gestix

  13. MOLECULAR CLONING, BACULOVIRUS EXPRESSION, AND TISSUE DISTRIBUTION OF THE ZEBRAFISH ALDEHYDE DEHYDROGENASE 2

    E-Print Network [OSTI]

    Tullos, Desiree

    ABSTRACT: Ethanol is metabolized to acetaldehyde mainly by the alcohol de- hydrogenase pathway and is responsible for some of the deleterious effects of ethanol, is further oxidized to acetic acid by aldehyde zebrafish (Danio rerio) as a model for ethanol metabolism by cloning, expressing, and characterizing

  14. Systems for the expression of orthogonal translation components in eubacterial host cells

    DOE Patents [OSTI]

    Ryu, Youngha; Schultz, Peter G.

    2013-01-22T23:59:59.000Z

    The invention related to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural acids at genetically-programmed positions.

  15. Evaluation and Comparison of Clustering Algorithms in Analyzing ES Cell Gene Expression Data

    E-Print Network [OSTI]

    of clustering algorithms, including hierarchical clustering, k-means, PAM and SOM. The indices were homogeneity) reported their success with k-means algorithm, an approach that minimizes the overall within1 Evaluation and Comparison of Clustering Algorithms in Analyzing ES Cell Gene Expression Data

  16. Classification of Gene Expression Profiles: Comparison of Kmeans and Expectation Maximization Algorithms

    E-Print Network [OSTI]

    Granada, Universidad de

    of similarity of expression patterns. In this work we apply two clustering algorithms, K-means and Expecta- tion grouping microarray data: the K-means [7], a classic clustering algorithm based on Eu- clidean distance), proposed by Lauritzen in 1995 [10] as a variation the K-means. The main novelty of this technique

  17. Identifying the promoter features governing differential kinetics of co-regulated genes using fuzzy expressions

    E-Print Network [OSTI]

    Granada, Universidad de

    of the biggest challenges in genomics is the elucidation of the design principles controlling gene expression features used by a transcriptional regulator to differentially control genes that display distinctIdentifying the promoter features governing differential kinetics of co-regulated genes using fuzzy

  18. Characterization of Chemically Induced Liver Injuries Using Gene Co-Expression Modules

    E-Print Network [OSTI]

    Performance Computing Software Applications Institute, Telemedicine and Advanced Technology Research Center, U database containing organ-specific gene expression data matched to dose-dependent chemical exposures and Education through an interagency agreement between the U.S. Department of Energy and USAMRMC. The funders

  19. Novel regulatory cascades controlling expression of nitrogen-fixation genes in Geobacter sulfurreducens

    E-Print Network [OSTI]

    Lovley, Derek

    in these environments. Moreover, Geobacter species show promise for harvesting electricity from waste organic matterNovel regulatory cascades controlling expression of nitrogen-fixation genes in Geobacter Geobacter species often play an important role in bioremediation of environments contaminated with metals

  20. Systems for the expression of orthogonal translation components eubacterial host cells

    SciTech Connect (OSTI)

    Ryu, Youngha (San Diego, CA); Schultz, Peter G. (La Jolla, CA)

    2012-06-12T23:59:59.000Z

    The invention relates to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural amino acids at genetically-programmed positions.