National Library of Energy BETA

Sample records for high-throughput screening system

  1. Automated MEMS-based Drosophila embryo injection system for high-throughput RNAi screens

    E-Print Network [OSTI]

    Scott, Matthew

    Automated MEMS-based Drosophila embryo injection system for high-throughput RNAi screens Stefan developed an automated system based on microelectromechanical systems (MEMS) injectors for reliable mass

  2. High-throughput in vivo vertebrate screening

    E-Print Network [OSTI]

    Pardo-Martin, Carlos

    We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and ...

  3. High-Throughput and Combinatorial Screening of Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput and Combinatorial Screening of Hydrogen Storage Materials (presentation) High-Throughput and Combinatorial Screening of Hydrogen Storage Materials (presentation)...

  4. High throughput protein production screening

    DOE Patents [OSTI]

    Beernink, Peter T. (Walnut Creek, CA); Coleman, Matthew A. (Oakland, CA); Segelke, Brent W. (San Ramon, CA)

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  5. High Throughput/Combinatorial Screening of Hydrogen Storage Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Materials: UOP Approaches High ThroughputCombinatorial Screening of Hydrogen Storage Materials: UOP Approaches Presentation by Adriaan Sachtler from the High Throughput...

  6. High Throughput/Combinatorial Screening of Hydrogen Storage Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Materials (presentation) High ThroughputCombinatorial Screening of Hydrogen Storage Materials (presentation) Presented at the U.S. Department of Energy's Hydrogen Storage Meeting...

  7. High Throughput Combinatorial Screening of Biometic Metal-Organic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High Throughput Combinatorial Screening of Biometic Metal-Organic Materials for Military Hydrogen-Storage Materials (New Joint Miami UNREL DoDDLA Project) (presentation) High...

  8. Development and optimization of high-throughput zebrafish screening platform

    E-Print Network [OSTI]

    Koo, Bryan Kyo

    2010-01-01

    The high-throughput zebrafish screening platform is a revolutionary tool that enables subcellular precision in vivo whole animal screening of Danio Rerio. It can perform laser surgery and/or imaging in less than twenty ...

  9. First principles high throughput screening of oxynitrides for water-splitting photocatalysts

    E-Print Network [OSTI]

    Wu, Yabi

    2013-01-01

    In this paper, we present a first principles high throughput screening system to search for new water-splitting photocatalysts. We use the approach to screen through nitrides and oxynitrides. Most of the known photocatalytic ...

  10. High-throughput vertebrate total analysis/screening platform

    E-Print Network [OSTI]

    Chang, Tsung-Yao, Ph. D. Massachusetts Institute of Technology

    2012-01-01

    High-throughput screening (HTS) is seen as one of the most promising technologies to facilitate biomedical studies and pharmaceutical discoveries. Although large varieties of in vitro HTS technologies have opened great ...

  11. High-throughput screening for modulators of cellular contractile force

    E-Print Network [OSTI]

    Park, Chan Young; Tambe, Dhananjay; Chen, Bohao; Lavoie, Tera; Dowell, Maria; Simeonov, Anton; Maloney, David J; Marinkovic, Aleksandar; Tschumperlin, Daniel J; Burger, Stephanie; Frykenberg, Matthew; Butler, James P; Stamer, W Daniel; Johnson, Mark; Solway, Julian; Fredberg, Jeffrey J; Krishnan, Ramaswamy

    2014-01-01

    When cellular contractile forces are central to pathophysiology, these forces comprise a logical target of therapy. Nevertheless, existing high-throughput screens are limited to upstream signaling intermediates with poorly defined relationship to such a physiological endpoint. Using cellular force as the target, here we screened libraries to identify novel drug candidates in the case of human airway smooth muscle cells in the context of asthma, and also in the case of Schlemm's canal endothelial cells in the context of glaucoma. This approach identified several drug candidates for both asthma and glaucoma. We attained rates of 1000 compounds per screening day, thus establishing a force-based cellular platform for high-throughput drug discovery.

  12. High throughput chemical munitions treatment system

    DOE Patents [OSTI]

    Haroldsen, Brent L. (Manteca, CA); Stofleth, Jerome H. (Albuquerque, NM); Didlake, Jr., John E. (Livermore, CA); Wu, Benjamin C-P (San Ramon, CA)

    2011-11-01

    A new High-Throughput Explosive Destruction System is disclosed. The new system is comprised of two side-by-side detonation containment vessels each comprising first and second halves that feed into a single agent treatment vessel. Both detonation containment vessels further comprise a surrounding ventilation facility. Moreover, the detonation containment vessels are designed to separate into two half-shells, wherein one shell can be moved axially away from the fixed, second half for ease of access and loading. The vessels are closed by means of a surrounding, clam-shell type locking seal mechanisms.

  13. Agenda from the U.S. Department of Energy's High Throughput Screening...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Agenda from the U.S. Department of Energy's High Throughput...

  14. Automated High Throughput Protein Crystallization Screening at Nanoliter Scale and Protein Structural Study on Lactate Dehydrogenase

    SciTech Connect (OSTI)

    Fenglei Li

    2006-08-09

    The purposes of our research were: (1) To develop an economical, easy to use, automated, high throughput system for large scale protein crystallization screening. (2) To develop a new protein crystallization method with high screening efficiency, low protein consumption and complete compatibility with high throughput screening system. (3) To determine the structure of lactate dehydrogenase complexed with NADH by x-ray protein crystallography to study its inherent structural properties. Firstly, we demonstrated large scale protein crystallization screening can be performed in a high throughput manner with low cost, easy operation. The overall system integrates liquid dispensing, crystallization and detection and serves as a whole solution to protein crystallization screening. The system can dispense protein and multiple different precipitants in nanoliter scale and in parallel. A new detection scheme, native fluorescence, has been developed in this system to form a two-detector system with a visible light detector for detecting protein crystallization screening results. This detection scheme has capability of eliminating common false positives by distinguishing protein crystals from inorganic crystals in a high throughput and non-destructive manner. The entire system from liquid dispensing, crystallization to crystal detection is essentially parallel, high throughput and compatible with automation. The system was successfully demonstrated by lysozyme crystallization screening. Secondly, we developed a new crystallization method with high screening efficiency, low protein consumption and compatibility with automation and high throughput. In this crystallization method, a gas permeable membrane is employed to achieve the gentle evaporation required by protein crystallization. Protein consumption is significantly reduced to nanoliter scale for each condition and thus permits exploring more conditions in a phase diagram for given amount of protein. In addition, evaporation rate can be controlled or adjusted in this method during the crystallization process to favor either nucleation or growing processes for optimizing crystallization process. The protein crystals gotten by this method were experimentally proven to possess high x-ray diffraction qualities. Finally, we crystallized human lactate dehydrogenase 1 (H4) complexed with NADH and determined its structure by x-ray crystallography. The structure of LDH/NADH displays a significantly different structural feature, compared with LDH/NADH/inhibitor ternary complex structure, that subunits in LDH/NADH complex show open conformation or two conformations on the active site while the subunits in LDH/NADH/inhibitor are all in close conformation. Multiple LDH/NADH crystals were obtained and used for x-ray diffraction experiments. Difference in subunit conformation was observed among the structures independently solved from multiple individual LDH/NADH crystals. Structural differences observed among crystals suggest the existence of multiple conformers in solution.

  15. High Throughput Pretreatment and Enzyme Hydrolysis of Biomass: Screening Recalcitrance in Large Sample Populations (Presentation)

    SciTech Connect (OSTI)

    Decker, S. R.

    2010-10-01

    Presentation on the execution of the first high-throughput thermochemical pretreatment/enzyme digestion pipeline for screening biomass for recalcitrance.

  16. Infrastructure to support ultra high throughput biodosimetry screening after a radiological event

    E-Print Network [OSTI]

    Brenner, David Jonathan

    GUY GARTY1 , ANDREW KARAM2 , & DAVID J. BRENNER3 1 Radiological Research Accelerator Facility, Radiological Research Accelerator Facility, Nevis Laboratories, Columbia UniverInfrastructure to support ultra high throughput biodosimetry screening after a radiological event

  17. Software for supporting large scale data processing for High Throughput Screening

    E-Print Network [OSTI]

    Tai, David

    2011-12-31

    High Throughput Screening for is a valuable data generation technique for data driven knowledge discovery. Because the rate of data generation is so great, it is a challenge to cope with the demands of post experiment data analysis. This thesis...

  18. Methods and technologies for high-throughput and high-content small animal screening

    E-Print Network [OSTI]

    Rohde, Christopher, 1979-

    2012-01-01

    High-throughput and high-content screening (HTS and HCS) of whole animals requires their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis ...

  19. A Cell-Based High-Throughput Screen for Novel Chemical Inducers of Fetal Hemoglobin for Treatment of Hemoglobinopathies

    E-Print Network [OSTI]

    Peterson, Kenneth R.; Costa, Flá via C.; Fedosyuk, Halyna; Neades, Renee Y.; Chazelle, Allen M.; Zelenchuk, Lesya; Fonteles, Andrea H.; Dalal, Parmita; Roy, Anuradha; Chaguturu, Rathnam; Li, Biaoru; Pace, Betty S.

    2014-09-16

    , with minimal off-target effects, continues to be an elusive goal. One hurdle has been an assay amenable to a high-throughput screen (HTS) of chemicals that displays a robust c-globin off-on switch to identify potential lead compounds. Assay systems developed...

  20. High throughput solar cell ablation system

    DOE Patents [OSTI]

    Harley, Gabriel; Pass, Thomas; Cousins, Peter John; Viatella, John

    2014-10-14

    A solar cell is formed using a solar cell ablation system. The ablation system includes a single laser source and several laser scanners. The laser scanners include a master laser scanner, with the rest of the laser scanners being slaved to the master laser scanner. A laser beam from the laser source is split into several laser beams, with the laser beams being scanned onto corresponding wafers using the laser scanners in accordance with one or more patterns. The laser beams may be scanned on the wafers using the same or different power levels of the laser source.

  1. High throughput solar cell ablation system

    DOE Patents [OSTI]

    Harley, Gabriel; Pass, Thomas; Cousins, Peter John; Viatella, John

    2012-09-11

    A solar cell is formed using a solar cell ablation system. The ablation system includes a single laser source and several laser scanners. The laser scanners include a master laser scanner, with the rest of the laser scanners being slaved to the master laser scanner. A laser beam from the laser source is split into several laser beams, with the laser beams being scanned onto corresponding wafers using the laser scanners in accordance with one or more patterns. The laser beams may be scanned on the wafers using the same or different power levels of the laser source.

  2. Optical microplates for high-throughput screening of photosynthesis in lipid-producing algae{,

    E-Print Network [OSTI]

    Basu, Amar S.

    Optical microplates for high-throughput screening of photosynthesis in lipid- producing algae-producing algae of interest in 2nd generation biofuels. By conducting 96 experiments in parallel, photoirradiance the study of photosynthesis in algae. Societal challenges in energy sustainability have renewed interest

  3. User, Robot and Automation Evaluations in High-Throughput Biological Screening Processes

    E-Print Network [OSTI]

    Kaber, David B.

    User, Robot and Automation Evaluations in High-Throughput Biological Screening Processes Noa Segall Cognitive Ergonomics Laboratory North Carolina State University Raleigh, NC +1 (919) 513-4494 nsegall@ncsu.edu Rebecca S. Green Cognitive Ergonomics Laboratory North Carolina State University Raleigh, NC +1 (919) 513

  4. Droplet microfluidic technology for single-cell high-throughput screening

    E-Print Network [OSTI]

    Perrimon, Norbert

    Droplet microfluidic technology for single-cell high-throughput screening Eric Brouzesa,b,1 (received for review March 31, 2009) We present a droplet-based microfluidic technology that enables high our droplet microfluidic platform is modular, robust, uses no moving parts, and has a wide range

  5. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2015-01-01

    An acoustic high-throughput screening method is described for harvesting protein crystals and combining the protein crystals with chemicals such as a fragment library. Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s{sup ?1}) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  6. Computational Proteomics: High-throughput Analysis for Systems Biology

    SciTech Connect (OSTI)

    Cannon, William R.; Webb-Robertson, Bobbie-Jo M.

    2007-01-03

    High-throughput (HTP) proteomics is a rapidly developing field that offers the global profiling of proteins from a biological system. The HTP technological advances are fueling a revolution in biology, enabling analyses at the scales of entire systems (e.g., whole cells, tumors, or environmental communities). However, simply identifying the proteins in a cell is insufficient for understanding the underlying complexity and operating mechanisms of the overall system. Systems level investigations are relying more and more on computational analyses, especially in the field of proteomics generating large-scale global data.

  7. High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins

    DOE Patents [OSTI]

    Kim, Sung-Hou (Moraga, CA); Kim, Rosalind (Moraga, CA); Jancarik, Jamila (Walnut Creek, CA)

    2012-01-31

    An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.

  8. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; et al

    2014-06-01

    Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from themore »inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.« less

  9. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2014-06-01

    Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  10. The Materials genome : rapid materials screening for renewable energy using high-throughput density functional theory

    E-Print Network [OSTI]

    Jain, Anubhav, Ph.D. Massachusetts Institute of Technology

    2011-01-01

    This thesis relates to the emerging field of high-throughput density functional theory (DFT) computation for materials design and optimization. Although highthroughput DFT is a promising new method for materials discovery, ...

  11. High-throughput screening of perovskite alloys for piezoelectric performance and thermodynamic stability

    E-Print Network [OSTI]

    Armiento, Rickard R.

    We screen a large chemical space of perovskite alloys for systems with optimal properties to accommodate a morphotropic phase boundary (MPB) in their composition-temperature phase diagram, a crucial feature for high ...

  12. Droplet microfluidic technology for single-cell high-throughput screening

    E-Print Network [OSTI]

    Higgins, Darren

    , Lexington, MA 02421; and bGenetics Department, Harvard Medical School and cHoward Hughes Medical Institute oil and enables the digital manipulation of these reactors at a very high-throughput. Here, we by an immiscible oil. The advantages of this droplet- based technique include the physical and chemical isolation

  13. A Bayesian Approach to Calibrating High-Throughput Virtual Screening Results and Application to Organic Photovoltaic Materials

    E-Print Network [OSTI]

    Pyzer-Knapp, Edward O; Aspuru-Guzik, Alan

    2015-01-01

    A novel approach for calibrating quantum-chemical properties determined as part of a high-throughput virtual screen to experimental analogs is presented. Information on the molecular graph is extracted through the use of extended connectivity fingerprints, and exploited using a Gaussian process to calibrate both electronic properties such as frontier orbital energies, and optical gaps and device properties such as short circuit current density, open circuit voltage and power conversion efficiency. The Bayesian nature of this process affords a value for uncertainty in addition to each calibrated value. This allows the researcher to gain intuition about the model as well as the ability to respect its bounds.

  14. Constant pressure high throughput membrane permeation testing system

    DOE Patents [OSTI]

    Albenze, Erik J.; Hopkinson, David P.; Luebke, David R.

    2014-09-02

    The disclosure relates to a membrane testing system for individual evaluation of a plurality of planar membranes subjected to a feed gas on one side and a sweep gas on a second side. The membrane testing system provides a pressurized flow of a feed and sweep gas to each membrane testing cell in a plurality of membrane testing cells while a stream of retentate gas from each membrane testing cell is ported by a retentate multiport valve for sampling or venting, and a stream of permeate gas from each membrane testing cell is ported by a permeate multiport valve for sampling or venting. Back pressure regulators and mass flow controllers act to maintain substantially equivalent gas pressures and flow rates on each side of the planar membrane throughout a sampling cycle. A digital controller may be utilized to position the retentate and permeate multiport valves cyclically, allowing for gas sampling of different membrane cells over an extended period of time.

  15. High-Throughput Assay of 9 Lysosomal Enzymes for Newborn Screening

    E-Print Network [OSTI]

    Gelb, Michael

    transfers before injection into a dual-column HPLC equipped with switching valves to direct the flow for LSD expanded newborn screening has passed in several states. Newborn screening for Pompe and Fabry

  16. Risk-based high-throughput chemical screening and prioritization using exposure models and in vitro bioactivity assays

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shin, Hyeong -Moo; Ernstoff, Alexi; Arnot, Jon A.; Wetmore, Barbara A.; Csiszar, Susan A.; Fantke, Peter; Zhang, Xianming; McKone, Thomas E.; Jolliet, Olivier; Bennett, Deborah H.

    2015-05-01

    We present a risk-based high-throughput screening (HTS) method to identify chemicals for potential health concerns or for which additional information is needed. The method is applied to 180 organic chemicals as a case study. We first obtain information on how the chemical is used and identify relevant use scenarios (e.g., dermal application, indoor emissions). For each chemical and use scenario, exposure models are then used to calculate a chemical intake fraction, or a product intake fraction, accounting for chemical properties and the exposed population. We then combine these intake fractions with use scenario-specific estimates of chemical quantity to calculate dailymore »intake rates (iR; mg/kg/day). These intake rates are compared to oral equivalent doses (OED; mg/kg/day), calculated from a suite of ToxCast in vitro bioactivity assays using in vitro-to-in vivo extrapolation and reverse dosimetry. Bioactivity quotients (BQs) are calculated as iR/OED to obtain estimates of potential impact associated with each relevant use scenario. Of the 180 chemicals considered, 38 had maximum iRs exceeding minimum OEDs (i.e., BQs > 1). For most of these compounds, exposures are associated with direct intake, food/oral contact, or dermal exposure. The method provides high-throughput estimates of exposure and important input for decision makers to identify chemicals of concern for further evaluation with additional information or more refined models.« less

  17. Integrated microfluidic device for single-cell high throughput screening in dynamic gene expression analysis

    E-Print Network [OSTI]

    Hui, Lawrence Kwan Yeung

    2008-01-01

    P.J. , & Lee, L.P. , Microfluidics-based systems biology.T.M. & Quake, S.R. , Microfluidics: Fluid physics at theand applications of microfluidics in biology. Annu Rev

  18. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System 

    E-Print Network [OSTI]

    Sobahi, Nebras MohammedKamal A.

    2014-07-22

    This thesis presents the development of a high-throughput microfluidic impedance spectroscopy platform for electrically detecting analyzing impedance measurements of non-contact and label free microdroplets. This microfluidic impedance spectroscopy...

  19. Improving the Yeast Three-Hybrid System for High-Throughput Target Discovery

    E-Print Network [OSTI]

    Bailey, Kyle

    2011-05-27

    -protein interactions. The yeast three-hybrid system is an attractive alternative that uses genetic tools to screen for protein-small molecule interactions in cellulo. This thesis describes efforts to improve the utility of the yeast three-hybrid system to screen...

  20. Implementation of a High-Throughput Screen for Identifying Small Molecules to Activate the Keap1-Nrf2-ARE Pathway

    E-Print Network [OSTI]

    Wu, Kai Connie; McDonald, Peter R.; Liu, Jie Jerry; Chaguturu, Rathnam; Klaassen, Curtis D.

    2012-10-08

    of these genes. To identify Nrf2 activators for the treatment of oxidative/electrophilic stress-induced diseases, the present study developed a high-throughput assay to evaluate Nrf2 activation using AREc32 cells that contain a luciferase gene under the control...

  1. Automated high-throughput flow-through real-time diagnostic system

    DOE Patents [OSTI]

    Regan, John Frederick

    2012-10-30

    An automated real-time flow-through system capable of processing multiple samples in an asynchronous, simultaneous, and parallel fashion for nucleic acid extraction and purification, followed by assay assembly, genetic amplification, multiplex detection, analysis, and decontamination. The system is able to hold and access an unlimited number of fluorescent reagents that may be used to screen samples for the presence of specific sequences. The apparatus works by associating extracted and purified sample with a series of reagent plugs that have been formed in a flow channel and delivered to a flow-through real-time amplification detector that has a multiplicity of optical windows, to which the sample-reagent plugs are placed in an operative position. The diagnostic apparatus includes sample multi-position valves, a master sample multi-position valve, a master reagent multi-position valve, reagent multi-position valves, and an optical amplification/detection system.

  2. Gasification of refuse derived fuel in the Battelle high throughput gasification system

    SciTech Connect (OSTI)

    Paisley, M.A.; Creamer, K.S.; Tweksbury, T.L.; Taylor, D.R. )

    1989-07-01

    This report presents the results of an experimental program to demonstrate the suitability of the Battelle High Throughput Gasification Process to non-wood biomass fuels. An extensive data base on wood gasification was generated during a multi-year experimental program. This data base and subsequent design and economic analysis activities led to the discussion to study the gasification character of other fuels. The specific fuel studied was refuse derived fuel (RDF) which is a prepared municipal solid waste (MSW). The use of RDF, while providing a valuable fuel, can also provide a solution to MSW disposal problems. Gasification of MSW provides advantages over land fill or mass burn technology since a more usable form of energy, medium Btu gas, is produced. Land filling of wastes produces no usable products and mass burning while greatly reducing the volume of wastes for disposal can produce only steam. This steam must be used on site or very nearby this limiting the potential locations for mass burn facilities. Such a gas, if produced from currently available supplies of MSW, can contribute 2 quads to the US energy supply. 3 refs., 12 figs., 7 tabs.

  3. A cell based high-throughput screening approach for the discovery of new inhibitors of respiratory syncytial virus

    E-Print Network [OSTI]

    Chung, Dong-Hoon; Moore, Blake P.; Matharu, Daljit S.; Golden, Jennifer E.; Maddox, Clinton; Rasmussen, Lynn; Sosa, Melinda I.; Ananthan, Subramaniam; White, E. Lucile; Jia, Fuli; Jonsson, Colleen B.; Severson, William E.

    2013-01-10

    -based detection system for signal endpoint (Cell Titer Glo®). The assay is sensitive and robust, with Z factors greater than 0.8, signal to background greater than 35, and signal to noise greater than 24. Utilizing this assay, 313,816 compounds from the Molecular...

  4. High Throughput Web Inspection System using Time-stretch Real-time Imaging

    E-Print Network [OSTI]

    Kim, Chanju

    2013-01-01

    successfully report a dark-field web inspection system withsample substrate for our dark-field web inspection systemweb inspection system exploiting time- stretch and utilizing the merits coming from dark-

  5. High throughput optical scanner

    DOE Patents [OSTI]

    Basiji, David A. (Seattle, WA); van den Engh, Gerrit J. (Seattle, WA)

    2001-01-01

    A scanning apparatus is provided to obtain automated, rapid and sensitive scanning of substrate fluorescence, optical density or phosphorescence. The scanner uses a constant path length optical train, which enables the combination of a moving beam for high speed scanning with phase-sensitive detection for noise reduction, comprising a light source, a scanning mirror to receive light from the light source and sweep it across a steering mirror, a steering mirror to receive light from the scanning mirror and reflect it to the substrate, whereby it is swept across the substrate along a scan arc, and a photodetector to receive emitted or scattered light from the substrate, wherein the optical path length from the light source to the photodetector is substantially constant throughout the sweep across the substrate. The optical train can further include a waveguide or mirror to collect emitted or scattered light from the substrate and direct it to the photodetector. For phase-sensitive detection the light source is intensity modulated and the detector is connected to phase-sensitive detection electronics. A scanner using a substrate translator is also provided. For two dimensional imaging the substrate is translated in one dimension while the scanning mirror scans the beam in a second dimension. For a high throughput scanner, stacks of substrates are loaded onto a conveyor belt from a tray feeder.

  6. Automated High Throughput Drug Target Crystallography

    SciTech Connect (OSTI)

    Rupp, B

    2005-02-18

    The molecular structures of drug target proteins and receptors form the basis for 'rational' or structure guided drug design. The majority of target structures are experimentally determined by protein X-ray crystallography, which as evolved into a highly automated, high throughput drug discovery and screening tool. Process automation has accelerated tasks from parallel protein expression, fully automated crystallization, and rapid data collection to highly efficient structure determination methods. A thoroughly designed automation technology platform supported by a powerful informatics infrastructure forms the basis for optimal workflow implementation and the data mining and analysis tools to generate new leads from experimental protein drug target structures.

  7. Vehicle Technologies Office Merit Review 2015: High-Throughput Study of Diffusion and Phase Transformation Kinetics of Magnesium-Based Systems For Automotive Cast Magnesium Alloys

    Broader source: Energy.gov [DOE]

    Presentation given by Ohio State University at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about high-throughput study...

  8. Abstract--Design aspects of a minimally invasive high-throughput automation system for radiation biodosimetry are

    E-Print Network [OSTI]

    biodosimetry are reported. The system, currently under development, relies on robotic devices and advanced high of the automation system is proposed based on the use of an input stage, a centrifuge module, a cell harvesting

  9. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    SciTech Connect (OSTI)

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomics system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.

  10. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomicsmore »system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.« less

  11. Robofurnace: A semi-automated laboratory chemical vapor deposition system for high-throughput nanomaterial synthesis and process discovery

    SciTech Connect (OSTI)

    Oliver, C. Ryan; Westrick, William; Koehler, Jeremy; Brieland-Shoultz, Anna; Anagnostopoulos-Politis, Ilias; Cruz-Gonzalez, Tizoc [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States)] [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States); Hart, A. John, E-mail: ajhart@mit.edu [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States); Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2013-11-15

    Laboratory research and development on new materials, such as nanostructured thin films, often utilizes manual equipment such as tube furnaces due to its relatively low cost and ease of setup. However, these systems can be prone to inconsistent outcomes due to variations in standard operating procedures and limitations in performance such as heating and cooling rates restrict the parameter space that can be explored. Perhaps more importantly, maximization of research throughput and the successful and efficient translation of materials processing knowledge to production-scale systems, relies on the attainment of consistent outcomes. In response to this need, we present a semi-automated lab-scale chemical vapor deposition (CVD) furnace system, called “Robofurnace.” Robofurnace is an automated CVD system built around a standard tube furnace, which automates sample insertion and removal and uses motion of the furnace to achieve rapid heating and cooling. The system has a 10-sample magazine and motorized transfer arm, which isolates the samples from the lab atmosphere and enables highly repeatable placement of the sample within the tube. The system is designed to enable continuous operation of the CVD reactor, with asynchronous loading/unloading of samples. To demonstrate its performance, Robofurnace is used to develop a rapid CVD recipe for carbon nanotube (CNT) forest growth, achieving a 10-fold improvement in CNT forest mass density compared to a benchmark recipe using a manual tube furnace. In the long run, multiple systems like Robofurnace may be linked to share data among laboratories by methods such as Twitter. Our hope is Robofurnace and like automation will enable machine learning to optimize and discover relationships in complex material synthesis processes.

  12. Designing and Validating Ternary Pd Alloys for Optimum Sulfur/Carbon Resistance in Hydrogen Separation and Carbon Capture Membrane Systems Using High-Throughput Combinatorial Methods

    SciTech Connect (OSTI)

    Lewis, Amanda; Zhao, Hongbin; Hopkins, Scott

    2014-09-30

    This report summarizes the work completed under the U.S. Department of Energy Project Award No.: DE-FE0001181 titled “Designing and Validating Ternary Pd Alloys for Optimum Sulfur/Carbon Resistance in Hydrogen Separation and Carbon Capture Membrane Systems Using High-Throughput Combinatorial Methods.” The project started in October 1, 2009 and was finished September 30, 2014. Pall Corporation worked with Cornell University to sputter and test palladium-based ternary alloys onto silicon wafers to examine many alloys at once. With the specialized equipment at Georgia Institute of Technology that analyzed the wafers for adsorbed carbon and sulfur species six compositions were identified to have resistance to carbon and sulfur species. These compositions were deposited on Pall AccuSep® supports by Colorado School of Mines and then tested in simulated synthetic coal gas at the Pall Corporation. Two of the six alloys were chosen for further investigations based on their performance. Alloy reproducibility and long-term testing of PdAuAg and PdZrAu provided insight to the ability to manufacture these compositions for testing. PdAuAg is the most promising alloy found in this work based on the fabrication reproducibility and resistance to carbon and sulfur. Although PdZrAu had great initial resistance to carbon and sulfur species, the alloy composition has a very narrow range that hindered testing reproducibility.

  13. High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhou, Jizhong; He, Zhili; Yang, Yunfeng; Deng, Ye; Tringe, Susannah G.; Alvarez-Cohen, Lisa

    2015-01-27

    Understanding the structure, functions, activities and dynamics of microbial communities in natural environments is one of the grand challenges of 21st century science. To address this challenge, over the past decade, numerous technologies have been developed for interrogating microbial communities, of which some are amenable to exploratory work (e.g., high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e.g., phylogenetic and functional gene arrays). Here, we provide a critical review and synthesis of the most commonly applied “open-format” and “closed-format” detection technologies. We discuss their characteristics, advantages, and disadvantages within the context of environmental applications andmore »focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference genomes are few. In addition, we discuss crucial issues and considerations associated with applying complementary high-throughput molecular technologies to address important ecological questions.« less

  14. High Throughput Materials Characterization John M. Gregoire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    White Paper for Establishing a User Facility for Synchrotron-based High Throughput Materials Characterization John M. Gregoire 1 , Matthew J. Kramer 2 , Apurva Mehta 3 1 Joint...

  15. Attendees list from the U.S. Department of Energy's High Throughput...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Attendees list from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Attendees list from the U.S. Department of...

  16. ASYNCHRONOUS WAVE PIPELINES FOR HIGH THROUGHPUT DATAPATHS

    E-Print Network [OSTI]

    Huss, Sorin A.

    ASYNCHRONOUS WAVE PIPELINES FOR HIGH THROUGHPUT DATAPATHS O. Hauck and S. A. Huss fhauck j hussg pipeline architecture for high­speed clockless computation is proposed. It features gate­level pipelining the advantages of wave pipelining and asynchronous operation, i. e. very high throughput at low latency while

  17. Approaches to High Throughput Physical Organic Chemistry 

    E-Print Network [OSTI]

    Portal, Christophe

    2008-01-01

    Over the past ten years, the development of High Throughput (HT) synthetic chemistry techniques has allowed the rapid preparation of libraries of hundreds to thousands of compounds. These tools are now extensively used for drug and material...

  18. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Robust, High-Throughput Analysis of Protein Structures Print Wednesday, 28 October 2009 00:00 Scientists have developed a...

  19. Potential of High-Throughput Experimentation with Ammonia Borane...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of High-Throughput Experimentation with Ammonia Borane Solid Hydrogen Storage Materials (presentation) Potential of High-Throughput Experimentation with Ammonia Borane Solid...

  20. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D (presentation) High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D (presentation)...

  1. CellVisualizer : exploring hierarchical, multi-dimensional data with applications to high-throughput microscopy

    E-Print Network [OSTI]

    Kang, InHan

    2006-01-01

    In this thesis, we present a system for visualizing hierarchical, multi-dimensional, memory-intensive datasets. Specifically, we designed an interactive system to visualize data collected by high-throughput microscopy and ...

  2. Chemistry & Biology Genome-Wide High-Throughput Mining

    E-Print Network [OSTI]

    Yin, Jun

    Chemistry & Biology Article Genome-Wide High-Throughput Mining of Natural-Product Biosynthetic Gene.01.006 SUMMARY We have developed a phage-display method for high-throughput mining of bacterial gene clus- ters

  3. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D On June 26, 2007 the Hydrogen...

  4. Chain Replication for Supporting High Throughput and Availability

    E-Print Network [OSTI]

    Keinan, Alon

    Chain Replication for Supporting High Throughput and Availability Robbert van Renesse rvr services that exhibit high throughput and availability with- out sacrificing strong consistency guarantees the perfor- mance characteristics of a prototype implementa- tion. Throughput, availability, and several

  5. Statistical Algorithms for High-throughput Biological Data /

    E-Print Network [OSTI]

    Jeong, Kyowon

    2013-01-01

    Statistical Algorithms for High-throughput Biological Data by Kyowon Jeong Doctor of Philosophy in Electrical Engineering (Communication Theory

  6. The Design of High-Throughput Asynchronous Pipelines

    E-Print Network [OSTI]

    The Design of High-Throughput Asynchronous Pipelines Montek Singh Department of Computer Science UNIVERSITY December 2001 #12;The Design of High-Throughput Asynchronous Pipelines Montek Singh ABSTRACT is on high-throughput asynchronous pipelines, since pipelining is at the heart of most high

  7. The Design of HighThroughput Asynchronous Pipelines

    E-Print Network [OSTI]

    The Design of High­Throughput Asynchronous Pipelines Montek Singh Department of Computer Science UNIVERSITY December 2001 #12; The Design of High­Throughput Asynchronous Pipelines Montek Singh ABSTRACT is on high­throughput asynchronous pipelines, since pipelining is at the heart of most high

  8. High-throughput sequencing of cytosine methylation in plant DNA

    E-Print Network [OSTI]

    Hardcastle, Thomas J.

    2013-06-07

    . Genome Res 2008, 18(7):1051–1063. 66. Bormann Chung CA, Boyd VL, McKernan KJ, Fu Y, Monighetti C, Peckham HE, Barker M: Whole methylome analysis by ultra-deep sequencing using two-base encoding. PloS One 2010, 5(2):e9320. 67. Ondov BD, Cochran C, Landers... reads. Bioinformatics 2013, 29(2):268–270. 70. Minoche AE, Dohm JC, Himmelbauer H: Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and genome analyzer systems. Genome Biol 2011, 12(11):R112. 71. Wang XV, Blades N, Ding J...

  9. MEMORY SUB-BANKING SCHEME FOR HIGH THROUGHPUT TURBO DECODER Mayank Tiwari, Yuming Zhu and Chaitali Chakrabarti

    E-Print Network [OSTI]

    Kambhampati, Subbarao

    level are needed in order to design low-power high- throughput Turbo decoders for mobile systems, the sliding win- dow (SW) approach has been proposed as an effective means of reduc- ing the decoding delay-banked implemen- tation of the SW-based approach that achieves high throughput, low decoding latency and reduced

  10. Novel System for Recalcitrance Screening Will Reduce Biofuels Production Costs; The Spectrum of Clean Energy Innovation (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2010-06-01

    Fact sheet describes a high-throughput screening process, developed at NREL, that enables researchers to screen a large variety of biomass feedstocks for traits that indicate they would easily convert to fermentable sugars.

  11. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that...

  12. High-throughput metagenomic technologies for complex microbial...

    Office of Scientific and Technical Information (OSTI)

    formats Prev Next Title: High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats You are accessing a document from...

  13. NETL Studies High Throughput Membrane Screening | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and economical method for gas separations from power plant flue gas streams. Polymer membranes and supported liquid membranes show great promise to solve problems in the...

  14. Microfluidic Technologies for High-Throughput Screening Applications

    E-Print Network [OSTI]

    Quake, Stephen R.

    -based dynamic formation of picoliter-sized water droplets in a continuously flowing oil-surfactant stream for all of his valuable combinatorial chemistry advice and the generous use of lab space and supplies and electronics. Second, I would like to thank a large number of professors outside of my field that directly

  15. High-throughput Microfluidic Screening Platforms for Microalgae Study 

    E-Print Network [OSTI]

    Kim, Hyun Soo

    2014-12-15

    Microalgae have been envisioned as a future source of renewable energy. Both fossil fuel depletion and environmental concern have drawn more interest in microalgal biofuels, but the production cost of these biofuels are not yet economically...

  16. High Throughput Combinatorial Screening of Biometic Metal-Organic Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancing ProgramsDepartment of¡High HIGHofWasteDepartmentfor

  17. High Throughput/Combinatorial Screening of Hydrogen Storage Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancing ProgramsDepartment of¡High

  18. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancing ProgramsDepartment of¡HighApproaches | Department of

  19. High-Throughput and Combinatorial Screening of Hydrogen Storage Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancingR Walls - Building America Topa High Binding Energy

  20. PARALLEL HIGH THROUGHPUT SOFT-OUTPUT SPHERE DECODER Q. Qi, C. Chakrabarti

    E-Print Network [OSTI]

    Kambhampati, Subbarao

    - putation complexity of only the list generator. Recently, a high speed systolic-like soft-output spherePARALLEL HIGH THROUGHPUT SOFT-OUTPUT SPHERE DECODER Q. Qi, C. Chakrabarti School of Electrical,chaitali}@asu.edu ABSTRACT Multiple-Input-Multiple-Output communication systems de- mand fast sphere decoding with high

  1. New Tools for High Throughput Chemistry and Biology 

    E-Print Network [OSTI]

    Galveia, Luciano Paulo Rosa

    2011-01-01

    New strategies for the high-throughput determination of protease substrate specificity using a dual colour microarray based format with a small library of FRET-based peptides were developed. Integral to this process was ...

  2. High-Throughput Program for the Discovery of NOx Reduction Catalysts...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput Program for the Discovery of NOx Reduction Catalysts High-Throughput Program for the Discovery of NOx Reduction Catalysts 2004 Diesel Engine Emissions Reduction...

  3. Systematic reconstruction of RNA functional motifs with high throughput microfluidics

    E-Print Network [OSTI]

    Quake, Stephen R.

    Systematic reconstruction of RNA functional motifs with high throughput microfluidics Lance Martin1 Hill, NC 27599 Abstract We present RNA-MITOMI, a microfluidic platform for integrated synthesis microfluidic platform such that the entire RNA library could be simultaneously synthesized and then assayed

  4. High throughput liquid absorption preconcentrator sampling instrument

    DOE Patents [OSTI]

    Zaromb, S.; Bozen, R.M.

    1992-12-22

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis. 12 figs.

  5. Updating Interconnection Screens for PV System Integration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Abraham Ellis, Roger Hill Sandia National Laboratories Tom Key, Kristen Nicole, Jeff Smith Electric Power Research Institute Updating Interconnection Screens for PV System...

  6. SAMDI Mass Spectrometry for High Throughput Discovery of Enzyme Function |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein StructuresMaintenance /

  7. High-Throughput Methodology for Discovery of Metal-Organic Frameworks...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint UC-BerkeleySymyx DoDDLA Project) (presentation) High-Throughput...

  8. A GPU-INSPIRED SOFT PROCESSOR FOR HIGH-THROUGHPUT ACCELERATION Jeffrey Kingyens and J. Gregory Steffan

    E-Print Network [OSTI]

    Steffan, J. Gregory

    ) is realizable on an XtremeData XD1000 FPGA-based accelerator system, we demonstrate that our soft processor can in the scientific, financial, and other data-driven arenas that will use them. Developing an accelerator designA GPU-INSPIRED SOFT PROCESSOR FOR HIGH-THROUGHPUT ACCELERATION Jeffrey Kingyens and J. Gregory

  9. Environmental characterization studies of a high-throughput wood gasifier

    SciTech Connect (OSTI)

    Chang, H.; Niemann, R.C.; Wilzbach, K.E.; Paisley, M.

    1983-01-01

    Potential environmental effects associated with thermochemical biomass gasification have been studied by Argonne National Laboratory in cooperation with Battelle Columbus Laboratories (BCL). A series of samples from the process research unit of an indirectly heated, high-throughput wood gasifier operated by BCL has been analyzed for potentially toxic organic compounds and trace elements. The results indicate that, under the test-run conditions, the gasification of both pine and hardwood is accompanied by the formation of some oil, the heavier fraction of which gives a positive response in the Ames assay for mutagenicity and contains numerous phenols and polycyclic aromatic hydrocarbons, including some carcinogens. The implications of these observations are discussed.

  10. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and

  11. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast

  12. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein Structures Print Scientists have developed a

  13. Updating Interconnection Screens for PV System Integration

    SciTech Connect (OSTI)

    Coddington, M.; Mather, B.; Kroposki, B.; Lynn, K.; Razon, A.; Ellis, A.; Hill, R.; Key, T.; Nicole, K.; Smith, J.

    2012-02-01

    This white paper evaluates the origins and usefulness of the capacity penetration screen, offer short-term solutions which could effectively allow fast-track interconnection to many PV system applications, and considers longer-term solutions for increasing PV deployment levels in a safe and reliable manner while reducing or eliminating the emphasis on the penetration screen. Short-term and longer-term alternatives approaches are offered as examples; however, specific modifications to screening procedures should be discussed with stakeholders and must ultimately be adopted by state and federal regulatory bodies.

  14. Smart Screening System (S3) In Taconite

    SciTech Connect (OSTI)

    Daryoush Allaei

    2006-09-08

    The conventional screening machines used in processing plants have had undesirable high noise and vibration levels. They also have had unsatisfactorily low screening efficiency, high energy consumption, high maintenance cost, low productivity, and poor worker safety. These conventional vibrating machines have been used in almost every processing plant. Most of the current material separation technology uses heavy and inefficient electric motors with an unbalanced rotating mass to generate the shaking. In addition to being excessively noisy, inefficient, and high-maintenance, these vibrating machines are often the bottleneck in the entire process. Furthermore, these motors, along with the vibrating machines and supporting structure, shake other machines and structures in the vicinity. The latter increases maintenance costs while reducing worker health and safety. The conventional vibrating fine screens at taconite processing plants have had the same problems as those listed above. This has resulted in lower screening efficiency, higher energy and maintenance cost, and lower productivity and workers safety concerns. The focus of this work is on the design of a high performance screening machine suitable for taconite processing plants. SmartScreens{trademark} technology uses miniaturized motors, based on smart materials, to generate the shaking. The underlying technologies are Energy Flow Control{trademark} and Vibration Control by Confinement{trademark}. These concepts are used to direct energy flow and confine energy efficiently and effectively to the screen function. The SmartScreens{trademark} technology addresses problems related to noise and vibration, screening efficiency, productivity, and maintenance cost and worker safety. Successful development of SmartScreens{trademark} technology will bring drastic changes to the screening and physical separation industry. The final designs for key components of the SmartScreens{trademark} have been developed. The key components include smart motor and associated electronics, resonators, and supporting structural elements. It is shown that the smart motors have an acceptable life and performance. Resonator (or motion amplifier) designs are selected based on the final system requirement and vibration characteristics. All the components for a fully functional prototype are fabricated and have been tested.

  15. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    E-Print Network [OSTI]

    Wu, Zhigang

    2009-01-01

    Soft inertial microfluidics for high throughput separation1 Introduction Microfluidics has gained significant advancesof mammalian cells using microfluidics 3,4 , there have been

  16. CONTINUOUS ROLL-TO-ROLL SERPENTINE DEPOSITION FOR HIGH THROUGHPUT a-Si PV MANUFACTURING

    E-Print Network [OSTI]

    Deng, Xunming

    CONTINUOUS ROLL-TO-ROLL SERPENTINE DEPOSITION FOR HIGH THROUGHPUT a-Si PV MANUFACTURING M. Izu, H. When this technique is incorporated into a continuous roll- to-roll PV manufacturing process a number of advantages in a fully automated high throughput PV module production plant [l-18

  17. Improving Bounds on the Football Pool Problem via Symmetry Reduction and High-Throughput Computing

    E-Print Network [OSTI]

    Linderoth, Jeffrey T.

    Improving Bounds on the Football Pool Problem via Symmetry Reduction and High-Throughput Computing of Wisconsin-Madison, 1210 W. Dayton St., Madison, WI 53706, USA, gthain@cs.wisc.edu The Football Pool Problem theory. Key words: Football Pool Problem; High-Throughput Computing; Branch-and-Bound; Con- dor; Master

  18. HPAZ: a High-throughput Pipeline Architecture of ZUC in Zongbin Liu1

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    HPAZ: a High-throughput Pipeline Architecture of ZUC in Hardware Zongbin Liu1 , Neng Gao1 , Jiwu@ist.psu.edu Abstract. In this paper, we propose a high-throughput pipeline architecture of the stream cipher ZUC which significantly reduces operating frequency. In this paper we design a mixed two-stage pipeline architecture which

  19. Assessing the Thermoelectric Properties of Sintered Compounds via High-Throughput Ab-Initio Calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    Assessing the Thermoelectric Properties of Sintered Compounds via High-Throughput Ab Database have been considered as nanograined, sintered-powder thermoelectrics with the high-throughput ab the electronic band gap and the carrier effective mass, and that the probability of having large thermoelectric

  20. Heterogeneous High Throughput Scientific Computing with APM X-Gene and Intel Xeon Phi

    E-Print Network [OSTI]

    David Abdurachmanov; Brian Bockelman; Peter Elmer; Giulio Eulisse; Robert Knight; Shahzad Muzaffar

    2014-10-10

    Electrical power requirements will be a constraint on the future growth of Distributed High Throughput Computing (DHTC) as used by High Energy Physics. Performance-per-watt is a critical metric for the evaluation of computer architectures for cost- efficient computing. Additionally, future performance growth will come from heterogeneous, many-core, and high computing density platforms with specialized processors. In this paper, we examine the Intel Xeon Phi Many Integrated Cores (MIC) co-processor and Applied Micro X-Gene ARMv8 64-bit low-power server system-on-a-chip (SoC) solutions for scientific computing applications. We report our experience on software porting, performance and energy efficiency and evaluate the potential for use of such technologies in the context of distributed computing systems such as the Worldwide LHC Computing Grid (WLCG).

  1. Constant pressure high throughput membrane permeation testing system

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing BacteriaConnect Collider Tests ofO y''Softening-Bipolaron''Article) |(Patent) |

  2. Constant pressure high throughput membrane permeation testing system

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing BacteriaConnect Collider Tests ofO y''Softening-Bipolaron''Article) |(Patent)

  3. Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates

    SciTech Connect (OSTI)

    Hamilton-Brehm, Scott; Vishnivetskaya, Tatiana A; Allman, Steve L; Mielenz, Jonathan R; Elkins, James G

    2012-01-01

    Flow cytometry (FCM) techniques have been developed for sorting mesophilic organisms, but the difficulty increases if the target microbes are thermophilic anaerobes. We demonstrate a reliable, high-throughput method of screening thermophilic anaerobic organisms using FCM and 96-well plates for growth on biomass-relevant substrates. The method was tested using the cellulolytic thermophiles Clostridium ther- mocellum (Topt = 55 C), Caldicellulosiruptor obsidiansis (Topt = 78 C) and the fermentative hyperthermo- philes, Pyrococcus furiosus (Topt = 100 C) and Thermotoga maritima (Topt = 80 C). Multi-well plates were incubated at various temperatures for approximately 72 120 h and then tested for growth. Positive growth resulting from single cells sorted into individual wells containing an anaerobic medium was verified by OD600. Depending on the growth substrate, up to 80 % of the wells contained viable cultures, which could be transferred to fresh media. This method was used to isolate thermophilic microbes from Rabbit Creek, Yellowstone National Park (YNP), Wyoming. Substrates for enrichment cultures including crystalline cellulose (Avicel), xylan (from Birchwood), pretreated switchgrass and Populus were used to cultivate organisms that may be of interest to lignocellulosic biofuel production.

  4. Metal Organic Framework Research: High Throughput Discovery of Robust Metal Organic Framework for CO2 Capture

    SciTech Connect (OSTI)

    None

    2010-08-01

    IMPACCT Project: LBNL is developing a method for identifying the best metal organic frameworks for use in capturing CO2 from the flue gas of coal-fired power plants. Metal organic frameworks are porous, crystalline compounds that, based on their chemical structure, vary considerably in terms of their capacity to grab hold of passing CO2 molecules and their ability to withstand the harsh conditions found in the gas exhaust of coal-fired power plants. Owing primarily to their high tunability, metal organic frameworks can have an incredibly wide range of different chemical and physical properties, so identifying the best to use for CO2 capture and storage can be a difficult task. LBNL uses high-throughput instrumentation to analyze nearly 100 materials at a time, screening them for the characteristics that optimize their ability to selectively adsorb CO2 from coal exhaust. Their work will identify the most promising frameworks and accelerate their large-scale commercial development to benefit further research into reducing the cost of CO2 capture and storage.

  5. Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing

    E-Print Network [OSTI]

    Larman, Harry Benjamin

    2012-01-01

    High throughput methods in molecular biology have changed the landscape of biomedical research. In particular, advances in massively parallel DNA sequencing and synthesis technologies are defining our genomes and the ...

  6. Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs)

    E-Print Network [OSTI]

    Xu, F.

    Cell-based biosensors (CBBs) are becoming an important tool for biosecurity applications and rapid diagnostics. For current CBBs technology, cell immobilization and high throughput fabrication are the main challenges. To ...

  7. Engineering a single cell microarray platform for high throughput DNA damage and repair analysis

    E-Print Network [OSTI]

    Weingeist, David McGregor

    2012-01-01

    DNA damage contributes to cancer, aging, and heritable diseases. Ironically, DNA damaging agents are also commonly used in current cancer treatment. We therefore need robust, high throughput, and inexpensive tools for ...

  8. An instrument for high-throughput measurements of fiber mechanical properties

    E-Print Network [OSTI]

    Kristofek, Grant William, 1980-

    2005-01-01

    In this thesis, an instrument is designed and constructed for the purpose of measuring the mechanical properties of single fibers. The instrument is intended to provide high throughput measurement of single fiber geometric ...

  9. Stochastic Forecasts Achieve High Throughput and Low Delay over Cellular Networks

    E-Print Network [OSTI]

    Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Balakrishnan, Hari; Winstein, Keith J.; Sivaraman Kaushalram, Anirudh; Balakrishnan, Hari

    Sprout is an end-to-end transport protocol for interactive applications that desire high throughput and low delay. Sprout works well over cellular wireless networks, where link speeds change dramatically with time, and ...

  10. Inertio-elastic focusing of bioparticles in microchannels at high throughput

    E-Print Network [OSTI]

    Lim, Eugene J.

    Controlled manipulation of particles from very large volumes of fluid at high throughput is critical for many biomedical, environmental and industrial applications. One promising approach is to use microfluidic technologies ...

  11. A microfluidic device for high throughput bacterial biofilm studies Jeongyun Kim,a

    E-Print Network [OSTI]

    Wood, Thomas K.

    A microfluidic device for high throughput bacterial biofilm studies Jeongyun Kim,a Manjunath Hegde of biofilm community formation. Here, we describe the development of a PDMS-based two-layer microfluidic flow

  12. SAMNet: a network-based approach to integrate multi-dimensional high throughput datasets

    E-Print Network [OSTI]

    Spencer, Sarah J.

    The rapid development of high throughput biotechnologies has led to an onslaught of data describing genetic perturbations and changes in mRNA and protein levels in the cell. Because each assay provides a one-dimensional ...

  13. A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial arrays

    E-Print Network [OSTI]

    Sun, Yu

    A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial8, Canada b Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College 16 September 2009 Available online 9 October 2009 Keywords: Hydrogel Micropatterning Biomaterial

  14. Microelectroporation device for genomic screening

    DOE Patents [OSTI]

    Perroud, Thomas D.; Renzi, Ronald F.; Negrete, Oscar; Claudnic, Mark R.

    2014-09-09

    We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

  15. Statistical Algorithms for High-throughput Biological Data /

    E-Print Network [OSTI]

    Jeong, Kyowon

    2013-01-01

    Ph. D. in Electrical Engineering (Communication Theory andin Electrical Engineering (Communication Theory and Systems)in Electrical Engineering (Communication Theory and Systems)

  16. A search model for topological insulators with high-throughput robustness descriptors

    SciTech Connect (OSTI)

    Yang, Kesong; Setyawan, Wahyu; Wang, Shidong; Nardelli, Marco B.; Curtarolo, Stephano

    2012-05-13

    Topological insulators (TI) are becoming one of the most studied classes of novel materials because of their great potential for applications ranging from spintronics to quantum computers. To fully integrate TI materials in electronic devices, high-quality epitaxial single-crystalline phases with sufficiently large bulk bandgaps are necessary. Current efforts have relied mostly on costly and time-consuming trial-and-error procedures. Here we show that by defining a reliable and accessible descriptor {cflx X}TI, which represents the topological robustness or feasibility of the candidate, and by searching the quantum materials repository aflowlib.org, we have automatically discovered 28 TIs (some of them already known) in five different symmetry families. These include peculiar ternary halides, Cs{l_brace}Sn,Pb,Ge{r_brace}{l_brace}Cl,Br,I{r_brace}{sub 3}, which could have been hardly anticipated without high-throughput means. Our search model, by relying on the significance of repositories in materials development, opens new avenues for the discovery of more TIs in different and unexplored classes of systems.

  17. A microreactor array for spatially resolved measurement of catalytic activity for high-throughput catalysis science

    SciTech Connect (OSTI)

    Kondratyuk, Petro; Gumuslu, Gamze; Shukla, Shantanu; Miller, James B.; Morreale, Bryan D.; Gellman, Andrew J.

    2013-04-01

    We describe a 100 channel microreactor array capable of spatially resolved measurement of catalytic activity across the surface of a flat substrate. When used in conjunction with a composition spread alloy film (CSAF, e.g. Pd{sub x}Cu{sub y}Au{sub 1-x-y}) across which component concentrations vary smoothly, such measurements permit high-throughput analysis of catalytic activity and selectivity as a function of catalyst composition. In the reported implementation, the system achieves spatial resolution of 1 mm{sup 2} over a 10×10 mm{sup 2} area. During operation, the reactant gases are delivered at constant flow rate to 100 points of differing composition on the CSAF surface by means of a 100-channel microfluidic device. After coming into contact with the CSAF catalyst surface, the product gas mixture from each of the 100 points is withdrawn separately through a set of 100 isolated channels for analysis using a mass spectrometer. We demonstrate the operation of the device on a Pd{sub x}Cu{sub y}Au{sub 1-x-y} CSAF catalyzing the H{sub 2}-D{sub 2} exchange reaction at 333 K. In essentially a single experiment, we measured the catalytic activity over a broad swathe of concentrations from the ternary composition space of the Pd{sub x}Cu{sub y}Au{sub 1-x-y} alloy.

  18. Synthesis and characterization of four new metal 5-phosphonoisophthalates discovered by high-throughput experimentation

    SciTech Connect (OSTI)

    Bauer, Sebastian [Institute of Inorganic Chemistry, Christian-Albrechts-University, Otto-Hahn-Platz 6/7, D 24098 Kiel (Germany); Stock, Norbert [Institute of Inorganic Chemistry, Christian-Albrechts-University, Otto-Hahn-Platz 6/7, D 24098 Kiel (Germany)], E-mail: stock@ac.uni-kiel.de

    2007-11-15

    A new ligand, 5-diethylphosphonoisophthalic acid ((HOOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}(C{sub 2}H{sub 5}){sub 2}, H{sub 2}Et{sub 2}L), for the hydrothermal synthesis of inorganic-organic hybrid compounds was prepared and characterized by NMR-spectroscopy. Its in situ hydrolysis leads to the corresponding 5-phosphonoisophthalic acid ((HOOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}H{sub 2}, H{sub 4}L). Applying high-throughput methods, different di- and trivalent metal salts for the synthesis of crystalline metal phosphonates based on H{sub 2}Et{sub 2}L have been screened. From the resulting discovery library, single-crystals of four new compounds, [Sm{sub 2}(H{sub 2}O){sub 4}(H(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}){sub 2}].2H{sub 2}O (1), [Cu{sub 3}(H{sub 2}O)(H(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}){sub 2}].2H{sub 2}O (2), Ca{sub 2}(H{sub 2}O)[H(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}H]{sub 2} (3), and Ba{sub 2}(H{sub 2}O){sub 3}(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3} (4), have been isolated. The single-crystal structure determination of the title compounds shows H{sub 4}L to be a versatile ligand, exhibiting different types of coordination modes between the functional groups and the metal ions. A comparison of the structural features of the title compounds shows a varying degree of M-O-M connectivities. Thus, isolated metal-oxygen clusters (compounds 1 and 2), infinite M-O-M chains (compound 3), and infinite M-O-M layers (compound 4) are observed. The title compounds 1, 2, and 3 were further characterized by IR-spectroscopy, TG-, EDX-, and elemental chemical analysis. - Graphical abstract: Applying high-throughput methods, the new ligand 5-diethylphosphonoisophtalic acid, (HOOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}(C{sub 2}H{sub 5}){sub 2} (H{sub 2}Et{sub 2}L), was reacted with several di- and trivalent metal salts under hydrothermal conditions. Single-crystals of four new inorganic-organic hybrid compounds were isolated from the discovery library. The single-crystal structure analysis shows a varying M-O-M connectivity.

  19. Industrial co-generation through use of a medium BTU gas from biomass produced in a high throughput reactor

    SciTech Connect (OSTI)

    Feldmann, H.F.; Ball, D.A.; Paisley, M.A.

    1983-01-01

    A high-throughput gasification system has been developed for the steam gasification of woody biomass to produce a fuel gas with a heating value of 475 to 500 Btu/SCF without using oxygen. Recent developments have focused on the use of bark and sawdust as feedstocks in addition to wood chips and the testing of a new reactor concept, the so-called controlled turbulent zone (CTZ) reactor to increase gas production per unit of wood fed. Operating data from the original gasification system and the CTZ system are used to examine the preliminary economics of biomass gasification/gas turbine cogeneration systems. In addition, a ''generic'' pressurized oxygen-blown gasification system is evaluated. The economics of these gasification systems are compared with a conventional wood boiler/steam turbine cogeneration system.

  20. High-throughput metagenomic technologies for complex microbial...

    Office of Scientific and Technical Information (OSTI)

    within the context of environmental applications and focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference...

  1. Microfabricated arrays for high-throughput screening of cellular response to cyclic substrate deformation

    E-Print Network [OSTI]

    Sun, Yu

    and pathobiology. Introduction The large number of factors in the cellular milieu that can impact biological to other stimuli, including matrix proteins,12 chemical cues,13 and genetic therapies.14 In some cases

  2. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    Microporous and Mesoporous Materials 2001, (29) Occelli, M.Microporous and Mesoporous Materials 2012, (170) Ben, T. ;Zhou, H. -C. Chemistry of Materials 2010, (172) Yuan, D. ;

  3. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    parameters for MOF-5, Cu-BTC and ZIF-8 were from universalMOF materials, MOF-5 129 and Cu-BTC 130 , again at roomFor methane adsorption in Cu-BTC, however, WDA-Y reproduces

  4. Integrated microfluidic device for single-cell high throughput screening in dynamic gene expression analysis

    E-Print Network [OSTI]

    Hui, Lawrence Kwan Yeung

    2008-01-01

    the resulting fluid flow lies in the laminar flow regime andfluid flow at the microflu- idic scale was strictly laminar andof laminar flow is that mixing of two conjoin- ing fluids is

  5. A high-throughput three-dimensional cell migration assay for toxicity screening

    E-Print Network [OSTI]

    Diseases, University of Texas Health Science Center, Houston, TX 77030 USA. There is a growing demand,19­21 . These nanoparticles are nontoxic and do not induce an inflammatory cytokine (IL-6, IL-8) response by cells22 they interact and aggregate with each other to form larger 3D cultures. This method has been shown to induce

  6. A high-throughput in vivo micronucleus assay for genome instability screening in mice

    E-Print Network [OSTI]

    Balmus, Gabriel; Karp, Natasha A.; Ng, Bee Ling; Jackson, Stephen P.; Adams, David J.; McIntyre, Rebecca E.

    2014-12-31

    and detection of molecules involved in the response to exogenous DNA damaging agents DNA damage response (DDR) defects can be revealed by treating mice that show normal spontaneous micronucleus frequencies with genotoxic agents. Upon treatment, micronuclei... or for the identification of deficiencies that are only apparent, or amplified, in response to DDR challenge because they are only necessary for the response to exogenous DNA damaging 640 agents rather than endogenous DNA damaging agents. This procedure will enable...

  7. Evaluation of high throughput screening methods in picking up differences between cultivars of

    E-Print Network [OSTI]

    California at Riverside, University of

    Sciences, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark c National Renewable Energy Laboratory, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH of combined eco- nomic benefits of higher yields at milder pretreatment condi- tions and lower enzyme dosages

  8. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    the compressed natural gas (CNG) at the same temperature andcompressed natural gas tank (CNG). According to this table,Compressed Natural Gas (CNG) 136 , involve either cryogenic

  9. High throughput screen for cells with high extracellular metabolite consumption--secretion rates using microfluidic droplets

    E-Print Network [OSTI]

    Wang, Benjamin L. (Benjamin Lu chen)

    2009-01-01

    Metabolic engineering has contributed significantly to the improvement of strains for the industrial production of various compounds. Traditionally, enzymatic steps closely associated with the product-forming pathway have ...

  10. A High-throughput Screening Approach Identifies Selective Regulators of Il12b Transcription

    E-Print Network [OSTI]

    Doty, Kevin Richard

    2012-01-01

    antibody, in patients with psoriasis: 76-week results from a1994). T cells involved in psoriasis vulgaris belong to theBoehncke, W. -H. (2005). Psoriasis. The New England journal

  11. High-Throughput Screen of Natural Product Libraries for Hsp90 Inhibitors

    E-Print Network [OSTI]

    Davenport, Jason; Blach, Maurie; Galam, Lakshmi; Girgis, Antwan; Hall, Jessica; Blagg, Brian S. J.; Matts, Robert L.

    2014-03-03

    with the report by Whitesell and co-workers that benzoquinone ansamycins, natural products isolated from the soil actinomycetes species Streptomyces hygroscopicus, were inhibitors of Hsp90 and not tyrosine kinases [1]. In 1997, the crystal structure of the Hsp90...) by Hsp90 [2]. Subsequently in 1998, radicicol, an antibiotic isolated from the mycoparasitic fungus Humicola fuscoatra, was found to bind similarly to the N-terminal domain of Hsp90 [3,4], and with the additional co-crystal structure in 1999, the road...

  12. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    Vehicles Concerns over the national energy security and global climate change are calling for the development of alternative

  13. Combined Catalysis and Optical Screening for High Throughput Discovery of Solar Fuels Catalysts

    E-Print Network [OSTI]

    Faraon, Andrei

    ,b a Joint Center for Artificial Photosynthesis, California Institute of Technology, Pasadena, California discovery effort within the Joint Center for Artificial Photosynthesis (JCAP, http

  14. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    organic compounds and gas storage capabilities in nanoporousof materials for gas storage has been rather limited becausefree energy and gas storage and transport properties in

  15. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    organic frameworks for hydrogen storage Abstract Classicalcandidate: for DOE’s hydrogen storage target condition, it’shydrogen storage ..

  16. A high-throughput digital imaging screen for the discovery and directed evolution of oxygenases

    E-Print Network [OSTI]

    Arnold, Frances H.

    numbers of crude enzyme samples. Results: Horseradish peroxidase (HRP) couples the phenolic products in degradation of xenobiotics) nearly always function as large, multimeric protein complexes that are only

  17. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    Celzard, A. ; Fierro, V. Energy & Fuels 2005, 19, 573. (140)Snurr, R. Q. ; Hupp, J. T. Energy & Environmental ScienceH. -K. ; Zhou, H. -C. Energy & Environmental Science 2013,

  18. High-Throughput Screening Platform for Engineered Nanoparticle-Mediated Genotoxicity Using CometChip Technology

    E-Print Network [OSTI]

    Watson, Christa

    The likelihood of intentional and unintentional engineered nanoparticle (ENP) exposure has dramatically increased due to the use of nanoenabled products. Indeed, ENPs have been incorporated in many useful products and have ...

  19. Agenda from the U.S. Department of Energy's High Throughput Screening of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based Fuels Research at NREL AdvancedEnergyAdvocateRegisterHydrogen Storage

  20. Improved Algae-based Biorefining and High-throughput Screening of Algal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLAN FOR THE SITE-218 58 84 168 167 164 1982-2016ARM-SGP

  1. High-throughput methods for characterizing the immune repertoire

    E-Print Network [OSTI]

    Laserson, Uri

    2013-01-01

    The adaptive immune system is one of the primary mediators in almost every major human disease, including infections, cancer, autoimmunity, and inflammation-based disorders. It fundamentally functions as a molecular ...

  2. An apparatus for high throughput muscle cell experimentation

    E-Print Network [OSTI]

    Garcia-Webb, Michael G. (Michael Gregory)

    2006-01-01

    The cardiac ventricular muscle cell (myocyte) is a key experimental system for exploring the mechanical properties of the diseased and healthy heart. The myocyte experimental model provides a higher level of physiological ...

  3. MATHEMATICAL CHALLENGES IN HIGH-THROUGHPUT MICROCALORIMETER SPECTROSCOPY

    E-Print Network [OSTI]

    -Xavier Proulx, Universit´e de Montr´eal Yun Wei, University of Michigan Heng Zhu, University of Calgary Jielin sensor systems (Figure 1) have been developed at NIST, NASA, and else- where to measure the energy. These microcalorimeters have demonstrated relative energy resolution, depend- ing on the energy band, of better than 3

  4. METHODS AND APPLICATIONS High-throughput instant quantification of

    E-Print Network [OSTI]

    Ihee, Hyotcherl

    based on photoactive yellow protein turn off/on label Youngmin Kim,1,2 Prabhakar Ganesan,1 on the Photoactive yellow protein turn Off/On Label (POOL) system that can instantly quan- tify the concentration Gel Electrophoresis; POOL, PYP turn Off/On Label; PYP, Photoactive Yellow Protein; SUMO, Small

  5. UFlood: High-Throughput Flooding over Wireless Mesh Networks Jayashree Subramanian, Robert Morris, and Hari Balakrishnan

    E-Print Network [OSTI]

    UFlood: High-Throughput Flooding over Wireless Mesh Networks Jayashree Subramanian, Robert Morris--This paper proposes UFlood, a flooding protocol for wireless mesh networks. UFlood targets situations of completion of a flood to the slowest receiving node and total time spent transmitting. The key to achieving

  6. Achieving High Throughput Ground-to-UAV Transport via Parallel Links

    E-Print Network [OSTI]

    Kung, H. T.

    Achieving High Throughput Ground-to-UAV Transport via Parallel Links Chit-Kwan Lin, H. T. Kung in unmanned aerial vehicle (UAV) applications, is a challenge due to varying channel quality and extended link diversity; 802.11; link layer; transport layer; UAV; network coding I. INTRODUCTION In this paper, we focus

  7. Comparison of High-Throughput Electrochemical Methods for Testing Direct Methanol Fuel Cell Anode Electrocatalysts

    E-Print Network [OSTI]

    power applications, fuel cells must compete with commodity power sources such as fossil fuels power sources, and mobile/portable applications, fuel cells that utilize liquid fuels can representComparison of High-Throughput Electrochemical Methods for Testing Direct Methanol Fuel Cell Anode

  8. Knife-Edge Scanning Microscopy: High-throughput Imaging and Analysis of

    E-Print Network [OSTI]

    Choe, Yoonsuck

    : High-throughput Imaging and Analysis of Massive Volumes of Biological Microstructures Author 1 Yoonsuck Choe Author 1 - Address Department of Computer Science, Texas A&M Univ. 3112 TAMU, College Station, TX, 77843-3112, USA Author 1 - Email choe@tamu.edu Author 1 - Tel +1 979 845 5466 Author 2 Louise C. Abbott

  9. Grid Portal Interface for Interactive Use and Monitoring of High-Throughput Proteome

    E-Print Network [OSTI]

    Bourne, Philip E.

    Grid Portal Interface for Interactive Use and Monitoring of High-Throughput Proteome Annotation, #12;54 A. Shahab et al. portal solution for grid computing in life sciences under the auspices, and the use of a GridSpeed [6] generated iGAP application portal are described. The portal solution was part

  10. S-box pipelining using genetic algorithms for high-throughput AES implementations: How

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    S-box pipelining using genetic algorithms for high-throughput AES implementations: How fast can we. In this manuscript, we explore how Genetic Algorithms (GAs) can be used for pipelining the AES substitution box based the implementation area [5,6]. However, when considering fast hardware implementations, pipelining is an obvious

  11. TECHNICAL ADVANCE A high-throughput method for quantifying growth of

    E-Print Network [OSTI]

    Dangl, Jeff

    : Arabidopsis, resistance genes, Pseudomonas syringae, phytopathogenic bacteria, counting bacteria IntroductionTECHNICAL ADVANCE A high-throughput method for quantifying growth of phytopathogenic bacteria; email: dangl@email.unc.edu) Summary Measuring the growth of pathogenic bacteria in leaves is a mainstay

  12. ORIGINAL ARTICLE GeoChip 3.0 as a high-throughput tool for analyzing

    E-Print Network [OSTI]

    Thomas, David D.

    significantly changed with the plant species diversity. As expected, GeoChip 3.0 is a high-throughput powerful Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA and 8 Virtual Institute in microbial ecology Keywords: microarray; functional genes; microbial community; plant diversity Introduction

  13. ORIGINAL ARTICLE GeoChip 3.0 as a high-throughput tool for analyzing

    E-Print Network [OSTI]

    Hazen, Terry

    significantly changed with the plant species diversity. As expected, GeoChip 3.0 is a high-throughput powerful Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA and 8 Virtual Institute Keywords: microarray; functional genes; microbial community; plant diversity Introduction Microorganisms

  14. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Hantke, Max, F.

    Preprocessed detector images that were used for the paper "High-throughput imaging of heterogeneous cell organelles with an X-ray laser". The CXI file contains the entire recorded data - including both hits and blanks. It also includes down-sampled images and LCLS machine parameters. Additionally, the Cheetah configuration file is attached that was used to create the pre-processed data.

  15. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Hantke, Max, F.

    2014-11-17

    Preprocessed detector images that were used for the paper "High-throughput imaging of heterogeneous cell organelles with an X-ray laser". The CXI file contains the entire recorded data - including both hits and blanks. It also includes down-sampled images and LCLS machine parameters. Additionally, the Cheetah configuration file is attached that was used to create the pre-processed data.

  16. Journal of Power Sources 158 (2006) 6068 High throughput evaluation of perovskite-based anode catalysts

    E-Print Network [OSTI]

    Mukasyan, Alexander

    2006-01-01

    Journal of Power Sources 158 (2006) 60­68 High throughput evaluation of perovskite-based anode to 25 compositions simultaneously under DMFC conditions. It was found that the Sr-based perovskites.g. perovskites, cuprates, etc.) Corresponding author. Tel.: +1 765 494 4075; fax: +1 765 494 0805. E

  17. Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical

    E-Print Network [OSTI]

    Zhu, Xiangdong

    Oblique-incidence reflectivity difference microscope for label-free high-throughput detection (OI-RD) microscope, a form of polarization-modulated imaging ellipsometer, for label on the polarizer­ compensator­sample­analyzer scheme and under the off-null condition, a polarization-modulated OI

  18. The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural

    E-Print Network [OSTI]

    Hendrickson, Wayne A.

    The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform Springer Science+Business Media B.V. 2010 Abstract The New York Consortium on Membrane Protein Structure Core Laboratory, New York Structural Biology Center, New York, NY 10027, USA F. Mancia Á M. Zhou

  19. Opal: A Multi-radio Platform for High Throughput Wireless Sensor Networks

    E-Print Network [OSTI]

    Langendoen, Koen

    1 Opal: A Multi-radio Platform for High Throughput Wireless Sensor Networks Raja Jurdak, Kevin requirements. This letter presents the design of the versatile Opal platform that couples a Cortex M3 MCU sacrificing communication range. We present experiments that evaluate Opal's throughput and range when

  20. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    SciTech Connect (OSTI)

    Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

    2009-01-05

    We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

  1. High-throughput liquid-absorption preconcentrator sampling methods

    DOE Patents [OSTI]

    Zaromb, S.

    1994-07-12

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis. 12 figs.

  2. nAture methods | VOL.12 NO.1 | JANUARY2015 | 71 high-throughput methods based on chromosome conformation

    E-Print Network [OSTI]

    Cai, Long

    that of hi-c. coupling this method with dnA-capture technology provides a high-throughput approachArticles nAture methods | VOL.12 NO.1 | JANUARY2015 | 71 high-throughput methods based for targeted mapping of fine-scale chromatin architecture. We applied targeted dnase hi-c to characterize the 3

  3. Screening

    Office of Energy Efficiency and Renewable Energy (EERE)

    Screening is typically performed by an outside party or an independent renewable energy expert or team. It is a review of the possible technology options that identifies dead-ends and further...

  4. Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

    SciTech Connect (OSTI)

    Hura, Greg L.; Menon, Angeli L.; Hammel, Michal; Rambo, Robert P.; Poole II, Farris L.; Tsutakawa, Susan E.; Jenney Jr, Francis E.; Classen, Scott; Frankel, Kenneth A.; Hopkins, Robert C.; Yang, Sungjae; Scott, Joseph W.; Dillard, Bret D.; Adams, Michael W. W.; Tainer, John A.

    2009-07-20

    We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.

  5. Screening system and method of using same

    DOE Patents [OSTI]

    Jones, David A; Gresham, Christopher A; Basiliere, Marc L; Spates, James J; Rodacy, Philip J

    2014-04-15

    An integrated apparatus and method for screening an object for a target material is provided. The integrated apparatus comprises a housing and an integrated screener. The housing is positionable adjacent the object, and has a channel therethrough. The integrated screener is positionable in the housing, and comprises a fan, at least one filter, a heater and an analyzer. The fan is for drawing air carrying particles and vapor through the channel of the housing. The filter(s) is/are positionable in the channel of the housing for passage of the air therethrough. The filter(s) comprise(s) at least one metal foam having a plurality of pores therein for collecting and adsorbing a sample from the particles and vapor passing therethrough. The heater is for applying heat to the at least one metal foam whereby the collected sample is desorbed from the metal foam. The analyzer detects the target material from the desorbed sample.

  6. A 96-well microplate incorporating a replica molded microfluidic network integrated with photonic crystal biosensors for high throughput kinetic

    E-Print Network [OSTI]

    Cunningham, Brian

    crystal biosensors for high throughput kinetic biomolecular interaction analysis{; Charles J. Choi microfluidic channels, with integrated label-free photonic crystal biosensors as the bottom surface the volume of reagent required to functionalize the biosensor surface, while retaining compatibility

  7. Evaluation of Tavorite-Structured Cathode Materials for Lithium-Ion Batteries Using High-Throughput Computing

    E-Print Network [OSTI]

    Mueller, Tim

    Cathode materials with structure similar to the mineral tavorite have shown promise for use in lithium-ion batteries, but this class of materials is relatively unexplored. We use high-throughput density-functional-theory ...

  8. High-throughput data mined prediction of inorganic compounds and computational discovery of new lithium-ion battery cathode materials

    E-Print Network [OSTI]

    Hautier, Geoffroy (Geoffroy T. F.)

    2011-01-01

    The ability to computationally predict the properties of new materials, even prior to their synthesis, has been made possible due to the current accuracy of modern ab initio techniques. In some cases, high-throughput ...

  9. High Throughput Plasmid Sequencing with Illumina and CLC Bio (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema (OSTI)

    Athavale, Ajay [Monsanto

    2013-01-25

    Ajay Athavale (Monsanto) presents "High Throughput Plasmid Sequencing with Illumina and CLC Bio" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  10. Advances in high-throughput speed, low-latency communication for embedded instrumentation ( 7th Annual SFAF Meeting, 2012)

    ScienceCinema (OSTI)

    Jordan, Scott [Physik Instrumente

    2013-02-11

    Scott Jordan on "Advances in high-throughput speed, low-latency communication for embedded instrumentation" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  11. High Throughput Computing Impact on Meta Genomics (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    SciTech Connect (OSTI)

    Gore, Brooklin [Morgridge Institute for Research] [Morgridge Institute for Research

    2011-10-12

    This presentation includes a brief background on High Throughput Computing, correlating gene transcription factors, optical mapping, genotype to phenotype mapping via QTL analysis, and current work on next gen sequencing.

  12. High Throughput Computing Impact on Meta Genomics (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    ScienceCinema (OSTI)

    Gore, Brooklin [Morgridge Institute for Research

    2013-01-22

    This presentation includes a brief background on High Throughput Computing, correlating gene transcription factors, optical mapping, genotype to phenotype mapping via QTL analysis, and current work on next gen sequencing.

  13. High Throughput Plasmid Sequencing with Illumina and CLC Bio (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    SciTech Connect (OSTI)

    Athavale, Ajay [Monsanto] [Monsanto

    2012-06-01

    Ajay Athavale (Monsanto) presents "High Throughput Plasmid Sequencing with Illumina and CLC Bio" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  14. Managing System of Systems Requirements with a Requirements Screening Group

    SciTech Connect (OSTI)

    Ronald R. Barden

    2012-07-01

    Figuring out an effective and efficient way to manage not only your Requirement’s Baseline, but also the development of all your individual requirements during a Program’s/Project’s Conceptual and Development Life Cycle Stages can be both daunting and difficult. This is especially so when you are dealing with a complex and large System of Systems (SoS) Program with potentially thousands and thousands of Top Level Requirements as well as an equal number of lower level System, Subsystem and Configuration Item requirements that need to be managed. This task is made even more overwhelming when you have to add in integration with multiple requirements’ development teams (e.g., Integrated Product Development Teams (IPTs)) and/or numerous System/Subsystem Design Teams. One solution for tackling this difficult activity on a recent large System of Systems Program was to develop and make use of a Requirements Screening Group (RSG). This group is essentially a Team made up of co-chairs from the various Stakeholders with an interest in the Program of record that are enabled and accountable for Requirements Development on the Program/Project. The RSG co-chairs, often with the help of individual support team, work together as a Program Board to monitor, make decisions on, and provide guidance on all Requirements Development activities during the Conceptual and Development Life Cycle Stages of a Program/Project. In addition, the RSG can establish and maintain the Requirements Baseline, monitor and enforce requirements traceability across the entire Program, and work with other elements of the Program/Project to ensure integration and coordination.

  15. Solion ion source for high-efficiency, high-throughput solar cell manufacturing

    SciTech Connect (OSTI)

    Koo, John Binns, Brant; Miller, Timothy; Krause, Stephen; Skinner, Wesley; Mullin, James

    2014-02-15

    In this paper, we introduce the Solion ion source for high-throughput solar cell doping. As the source power is increased to enable higher throughput, negative effects degrade the lifetime of the plasma chamber and the extraction electrodes. In order to improve efficiency, we have explored a wide range of electron energies and determined the conditions which best suit production. To extend the lifetime of the source we have developed an in situ cleaning method using only existing hardware. With these combinations, source life-times of >200 h for phosphorous and >100 h for boron ion beams have been achieved while maintaining 1100 cell-per-hour production.

  16. Screening Pumping Systems for Energy Savings Opportunities 

    E-Print Network [OSTI]

    Casada, D.

    1999-01-01

    . Under the auspices of the Department of Energy's (DOE) Motor Challenge Program, prescreening guidance documents and a computer program called PSAT (Pumping System Assessment Tool) have been developed to help end users, consultants, and equipment...

  17. Development of a high-throughput microfluidic integrated microarray for the detection of chimeric bioweapons.

    SciTech Connect (OSTI)

    Sheppod, Timothy; Satterfield, Brent; Hukari, Kyle W.; West, Jason A. A.; Hux, Gary A.

    2006-10-01

    The advancement of DNA cloning has significantly augmented the potential threat of a focused bioweapon assault, such as a terrorist attack. With current DNA cloning techniques, toxin genes from the most dangerous (but environmentally labile) bacterial or viral organism can now be selected and inserted into robust organism to produce an infinite number of deadly chimeric bioweapons. In order to neutralize such a threat, accurate detection of the expressed toxin genes, rather than classification on strain or genealogical decent of these organisms, is critical. The development of a high-throughput microarray approach will enable the detection of unknowns chimeric bioweapons. The development of a high-throughput microarray approach will enable the detection of unknown bioweapons. We have developed a unique microfluidic approach to capture and concentrate these threat genes (mRNA's) upto a 30 fold concentration. These captured oligonucleotides can then be used to synthesize in situ oligonucleotide copies (cDNA probes) of the captured genes. An integrated microfluidic architecture will enable us to control flows of reagents, perform clean-up steps and finally elute nanoliter volumes of synthesized oligonucleotides probes. The integrated approach has enabled a process where chimeric or conventional bioweapons can rapidly be identified based on their toxic function, rather than being restricted to information that may not identify the critical nature of the threat.

  18. Thicker, more efficient superconducting strip-line detectors for high throughput macromolecules analysis

    SciTech Connect (OSTI)

    Casaburi, A.; Ejrnaes, M.; Cristiano, R.; Zen, N.; Ohkubo, M.; Pagano, S.

    2011-01-10

    Fast detectors with large area are required in time-of-flight mass spectrometers for high throughput analysis of biological molecules. We fabricated and characterized subnanosecond 1x1 mm{sup 2} NbN superconducting strip-line detectors. The influence of the strip-line thickness on the temporal characteristics and efficiency of the detector for the impacts of keV accelerated molecules is investigated. We find that the increase of thickness improves both efficiency and response time. In the thicker sample we achieved a rise time of 380 ps, a fall time of 1.38 ns, and a higher count rate. The physics involved in this behavior is investigated.

  19. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bell, Ronald E.

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ? 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, andmore »wavelength calibration.« less

  20. Enabling a High Throughput Real Time Data Pipeline for a Large Radio Telescope Array with GPUs

    E-Print Network [OSTI]

    Edgar, R G; Dale, K; Mitchell, D A; Ord, S M; Wayth, R B; Pfister, H; Greenhill, L J

    2010-01-01

    The Murchison Widefield Array (MWA) is a next-generation radio telescope currently under construction in the remote Western Australia Outback. Raw data will be generated continuously at 5GiB/sec. This high throughput motivates the development of on-site, real time processing and reduction in preference to archiving, transport and off-line processing. Maintaining real time operation will require a sustained performance of around 2.5TFLOP/sec (including convolutions, FFTs, interpolations and matrix multiplications). We describe a scalable heterogeneous computing pipeline implementation, exploiting both the high computing density and FLOP-per-Watt ratio of modern GPUs. The architecture is highly parallel within and across nodes, with all major processing elements performed by GPUs. Necessary scatter-gather operations along the pipeline are loosely synchronized and implemented in MPI. The MWA will be a frontier scientific instrument and a pathfinder for planned peta- and exascale facilities.

  1. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera

    SciTech Connect (OSTI)

    Bell, Ronald E.

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ? 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  2. Development and Operation of High-throughput Accurate-wavelength Lens-based Spectrometer

    SciTech Connect (OSTI)

    Bell, Ronald E

    2014-07-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy < 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  3. Development and operation of a high-throughput accurate-wavelength lens-based spectrometer

    SciTech Connect (OSTI)

    Bell, Ronald E.

    2014-11-15

    A high-throughput spectrometer for the 400–820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm{sup ?1} grating is matched with fast f/1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ?0.075 arc sec. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount at the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  4. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bell, Ronald E. [Princeton Plasma Physics Laboratory, Princeton, New Jersey 08543, USA

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy < 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  5. Metalloproteomics: High-Throughput Structural and Functional Annotation of Proteins in Structural Genomics

    SciTech Connect (OSTI)

    Shi,W.; Zhan, C.; Lgnatov, A.; Manjasetty, B.; Marinkovic, N.; Sullivan, M.; Huang, R.; Chance, M.; Li, H.; et al.

    2005-01-01

    A high-throughput method for measuring transition metal content based on quantitation of X-ray fluorescence signals was used to analyze 654 proteins selected as targets by the New York Structural GenomiX Research Consortium. Over 10% showed the presence of transition metal atoms in stoichiometric amounts; these totals as well as the abundance distribution are similar to those of the Protein Data Bank. Bioinformatics analysis of the identified metalloproteins in most cases supported the metalloprotein annotation; identification of the conserved metal binding motif was also shown to be useful in verifying structural models of the proteins. Metalloproteomics provides a rapid structural and functional annotation for these sequences and is shown to be {approx}95% accurate in predicting the presence or absence of stoichiometric metal content. The project's goal is to assay at least 1 member from each Pfam family; approximately 500 Pfam families have been characterized with respect to transition metal content so far.

  6. High-throughput generation, optimization and analysis of genome-scale metabolic models.

    SciTech Connect (OSTI)

    Henry, C. S.; DeJongh, M.; Best, A. A.; Frybarger, P. M.; Linsay, B.; Stevens, R. L.

    2010-09-01

    Genome-scale metabolic models have proven to be valuable for predicting organism phenotypes from genotypes. Yet efforts to develop new models are failing to keep pace with genome sequencing. To address this problem, we introduce the Model SEED, a web-based resource for high-throughput generation, optimization and analysis of genome-scale metabolic models. The Model SEED integrates existing methods and introduces techniques to automate nearly every step of this process, taking {approx}48 h to reconstruct a metabolic model from an assembled genome sequence. We apply this resource to generate 130 genome-scale metabolic models representing a taxonomically diverse set of bacteria. Twenty-two of the models were validated against available gene essentiality and Biolog data, with the average model accuracy determined to be 66% before optimization and 87% after optimization.

  7. been the key to their success is the use of high-throughput experiments to assess various zeo-

    E-Print Network [OSTI]

    Silver, Whendee

    it and the resulting pore structure. It could be that the hexamethonium molecules pack in such a way as to providebeen the key to their success is the use of high- throughput experiments to assess various zeo and shape of the resulting pores cor- responds to the size and shape of the organic molecule. ITQ-33

  8. PIPELINED PARALLEL ARCHITECTURE FOR HIGH THROUGHPUT MAP DETECTORS Ruwan Ratnayake, Gu-Yeon Wei and Aleksandar Kavcic

    E-Print Network [OSTI]

    Kavcic, Aleksandar

    PIPELINED PARALLEL ARCHITECTURE FOR HIGH THROUGHPUT MAP DETECTORS Ruwan Ratnayake, Gu-Yeon Wei performance close to the channel capacity limits. Deep pipelined architecture for the forward only method, where as FB-BCJR only allow sequential state metric computations. Parallelism facilitates pipelining, re

  9. Novel mixed polyanions lithium-ion battery cathode materials predicted by high-throughput ab initio computations

    E-Print Network [OSTI]

    Ceder, Gerbrand

    Novel mixed polyanions lithium-ion battery cathode materials predicted by high-throughput ab initio (>700 Wh/kg) cathode materials for lithium-ion batteries. 1 Introduction The widespread use of lithium compounds. Testing previously known lithium-containing compounds for battery properties can lead

  10. PIPA: A High-Throughput Pipeline for Protein Function Annotation Chenggang Yu, Valmik Desai, Nela Zavaljevski, and Jaques Reifman

    E-Print Network [OSTI]

    PIPA: A High-Throughput Pipeline for Protein Function Annotation Chenggang Yu, Valmik Desai, Nela of multisource predictions. We developed Pipeline for Protein Annotation (PIPA), a genome-wide protein function annotation pipeline that runs in a high-performance computing environment. PIPA integrates different tools

  11. Final Paper Drug Screening and Genomics

    E-Print Network [OSTI]

    Brutlag, Doug

    for a particular disease using only a computer. In this type of high-throughput screening (HTS), large libraries to the public after World War II. That same decade also witnessed the #12;growth of synthetic organic chemistry candidates was economically feasible. Synthetic organic chemistry was a very important advance at the time

  12. High-throughput liquid-absorption air-sampling apparatus and methods

    DOE Patents [OSTI]

    Zaromb, Solomon (95706 William Dr., Hinsdale, IL 60521)

    2000-01-01

    A portable high-throughput liquid-absorption air sampler [PHTLAAS] has an asymmetric air inlet through which air is drawn upward by a small and light-weight centrifugal fan driven by a direct current motor that can be powered by a battery. The air inlet is so configured as to impart both rotational and downward components of motion to the sampled air near said inlet. The PHTLAAS comprises a glass tube of relatively small size through which air passes at a high rate in a swirling, highly turbulent motion, which facilitates rapid transfer of vapors and particulates to a liquid film covering the inner walls of the tube. The pressure drop through the glass tube is <10 cm of water, usually <5 cm of water. The sampler's collection efficiency is usually >20% for vapors or airborne particulates in the 2-3.mu. range and >50% for particles larger than 4.mu.. In conjunction with various analyzers, the PHTLAAS can serve to monitor a variety of hazardous or illicit airborne substances, such as lead-containing particulates, tritiated water vapor, biological aerosols, or traces of concealed drugs or explosives.

  13. GeoChip 3.0: A High Throughput Tool for Analyzing Microbial Community, Composition, Structure, and Functional Activity

    SciTech Connect (OSTI)

    He, Zhili; Deng, Ye; Nostrand, Joy Van; Tu, Qichao; Xu, Meiying; Hemme, Chris; Wu, Liyou; Hazen, Terry; Zhou, Jizhong; Li, Xingyuan; Gentry, Terry; Yin, Yifeng; Liebich, Jost

    2010-05-17

    Microarray-based genomic technology has been widely used for microbial community analysis, and it is expected that microarray-based genomic technologies will revolutionize the analysis of microbial community structure, function and dynamics. A new generation of functional gene arrays (GeoChip 3.0) has been developed, with 27,812 probes covering 56,990 gene variants from 292 functional gene families involved in carbon, nitrogen, phosphorus and sulfur cycles, energy metabolism, antibiotic resistance, metal resistance, and organic contaminant degradation. Those probes were derived from 2,744, 140, and 262 species for bacteria, archaea, and fungi, respectively. GeoChip 3.0 has several other distinct features, such as a common oligo reference standard (CORS) for data normalization and comparison, a software package for data management and future updating, and the gyrB gene for phylogenetic analysis. Our computational evaluation of probe specificity indicated that all designed probes had a high specificity to their corresponding targets. Also, experimental analysis with synthesized oligonucleotides and genomic DNAs showed that only 0.0036percent-0.025percent false positive rates were observed, suggesting that the designed probes are highly specific under the experimental conditions examined. In addition, GeoChip 3.0 was applied to analyze soil microbial communities in a multifactor grassland ecosystem in Minnesota, USA, which demonstrated that the structure, composition, and potential activity of soil microbial communities significantly changed with the plant species diversity. All results indicate that GeoChip 3.0 is a high throughput powerful tool for studying microbial community functional structure, and linking microbial communities to ecosystem processes and functioning. To our knowledge, GeoChip 3.0 is the most comprehensive microarrays currently available for studying microbial communities associated with geobiochemical cycling, global climate change, bioenergy, agricuture, land use, ecosystem management, environmental cleanup and restoration, bioreactor systems, and human health.

  14. Coupled high-throughput functional screening and next generation sequencing for identification of plant polymer decomposing enzymes in metagenomic libraries

    E-Print Network [OSTI]

    2013-01-01

    730. doi: 10.1128/AEM. Cournoyer, B. , and Faure, D. (2003).MacGregor et al. , 2001; Cournoyer and Faure, 2003; Hill and

  15. High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons

    E-Print Network [OSTI]

    Nieland, Thomas J.

    The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation ...

  16. The Development of Microfabricated Microbial Fuel Cell Array as a High Throughput Screening Platform for Electrochemically Active Microbes 

    E-Print Network [OSTI]

    Hou, Huijie

    2012-02-14

    Microbial fuel cells (MFCs) are novel green technologies that convert chemical energy stored in biomass into electricity through microbial metabolisms. Both fossil fuel depletion and environmental concern have fostered significant interest in MFCs...

  17. Development of High-Throughput Screens to Target SAM-I Riboswitches (2014 DOE JGI Genomics of Energy & Environment Meeting)

    SciTech Connect (OSTI)

    Hickey, Scott [UC Berkely

    2014-03-19

    Scott Hickey of the University of California Berkeley speaks at the 9th Annual Genomics of Energy & Environment Meeting on March 20, 2014 in Walnut Creek, Calif.

  18. Development of High-throughput and Robust Microfluidic Live Cell Assay Platforms for Combination Drug and Toxin Screening 

    E-Print Network [OSTI]

    Wang, Han

    2012-02-14

    Combination chemotherapies that introduce multi-agent treatments to target cancer cells are emerging as new paradigms to overcome chemotherapy resistance and side effects involved with conventional monotherapies. In environmental toxicology...

  19. 10.1177/1087057105276989Kevorkov and MakarenkovSystematic Errors in High-Throughput Screening Statistical Analysis of Systematic Errors

    E-Print Network [OSTI]

    Makarenkov, Vladimir

    - mentaldatarequiresan efficientautomaticroutinefor theselection of hits. Unfortunately, random and systematic errors can

  20. High-Throughput Screening of Mg-Functionalized Metal-Organic Frameworks for Hydrogen Storage near Room Temperature

    E-Print Network [OSTI]

    Colón, Yamil J.; Fairen-Jimenez, David; Wilmer, Christopher E.; Snurr, Randall Q.

    2014-01-01

    The hydrogen storage capabilities of 18,383 porous crystalline structures possessing various degrees of Mg functionalization and diverse physical properties were assessed through combined grand canonical Monte Carlo (GCMC) and quantum mechanical...

  1. 'Sifting the significance from the data' - the impact of high-throughput genomic technologies on human genetics and health care

    E-Print Network [OSTI]

    Clarke, Angus J.; Cooper, David N.; Krawczak, Michael; Tyler-Smith, Chris; Wallace, Helen M.; Wilkie, Andrew O. M.; Raymond, Frances L.; Chadwick, Ruth; Craddock, Nick; John, Ros; Gallacher, John; Chiano, Mathias

    2012-08-02

    and ageing), and in response to environmental challenges (diet, stress). To this end, a number of ingenious genome-wide, high- throughput technologies have been developed. Some are based on the ability to selectively capture methylated fragments of the genome... consumes too much energy or mis- conceived arguments lead to the inappropriate applica- tion of theory in policy and practise across various walks of life, most especially psychiatry, education and the law. Second, and despite a full acceptance...

  2. Screening portal, system and method of using same

    SciTech Connect (OSTI)

    Linker, Kevin L.; Hunter, John A.; Brusseau, Charles A.

    2013-04-30

    A portal, system and method for screening an object for a target substance is provided. The portal includes an inflatable bladder expandable to form a test space for receiving the object and a plurality of nozzles positioned about the inflatable bladder. The nozzles are in fluid communication with a fluid source for directing air over the object whereby samples are removed from the object for examination. A collector is operatively connected to the inflatable bladder for collecting the samples removed from the object. A detector is operatively connected to the collector for examining the removed samples for the presence of the target substance. At least one preconcentrator may be operatively connected to the collector for concentrating the samples collected thereby.

  3. Bench-Top Engine System for Fast Screening of Alternative Fuels and Fuel Additives

    Broader source: Energy.gov [DOE]

    A bench-top engine testing system was used to fast screen the efficiency of fuel additives or fuel blends on NOx reduction

  4. High-Throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis

    SciTech Connect (OSTI)

    Wenwan Zhong

    2003-08-05

    Capillary electrophoresis (CE) offers many advantages over conventional analytical methods, such as speed, simplicity, high resolution, low cost, and small sample consumption, especially for the separation of enantiomers. However, chiral method developments still can be time consuming and tedious. They designed a comprehensive enantioseparation protocol employing neutral and sulfated cyclodextrins as chiral selectors for common basic, neutral, and acidic compounds with a 96-capillary array system. By using only four judiciously chosen separation buffers, successful enantioseparations were achieved for 49 out of 54 test compounds spanning a large variety of pKs and structures. Therefore, unknown compounds can be screened in this manner to identify optimal enantioselective conditions in just one rn. In addition to superior separation efficiency for small molecules, CE is also the most powerful technique for DNA separations. Using the same multiplexed capillary system with UV absorption detection, the sequence of a short DNA template can be acquired without any dye-labels. Two internal standards were utilized to adjust the migration time variations among capillaries, so that the four electropherograms for the A, T, C, G Sanger reactions can be aligned and base calling can be completed with a high level of confidence. the CE separation of DNA can be applied to study differential gene expression as well. Combined with pattern recognition techniques, small variations among electropherograms obtained by the separation of cDNA fragments produced from the total RNA samples of different human tissues can be revealed. These variations reflect the differences in total RNA expression among tissues. Thus, this Ce-based approach can serve as an alternative to the DNA array techniques in gene expression analysis.

  5. A truly unique high throughput, micro-volume protein analysis and characterisation system.

    E-Print Network [OSTI]

    Lebendiker, Mario

    and formulation studies. 2. Measurements with conventional analytical instrumentation are often slow and labour in the later stages of development.This can increase the risk of costly late stage problems or failure

  6. Integrated crystal mounting and alignment system for high-throughput biological crystallography

    DOE Patents [OSTI]

    Nordmeyer, Robert A.; Snell, Gyorgy P.; Cornell, Earl W.; Kolbe, William; Yegian, Derek; Earnest, Thomas N.; Jaklevic, Joseph M.; Cork, Carl W.; Santarsiero, Bernard D.; Stevens, Raymond C.

    2005-07-19

    A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

  7. Quantitative and high-throughput receptor affinity profiling system reveals distinct pathophysiological HIV-1 phenotypes

    E-Print Network [OSTI]

    Chikere, Kelechi C.

    2012-01-01

    in Journal of Virology: Palomares K, Pernet O, Vigant F,Immunity, In Press) Palomares K, Pernet O, Vigant F, Chikere

  8. Integrated crystal mounting and alignment system for high-throughput biological crystallography

    DOE Patents [OSTI]

    Nordmeyer, Robert A. (San Leandro, CA); Snell, Gyorgy P. (Richmond, CA); Cornell, Earl W. (Antioch, CA); Kolbe, William F. (Moraga, CA); Yegian, Derek T. (Oakland, CA); Earnest, Thomas N. (Berkeley, CA); Jaklevich, Joseph M. (Lafayette, CA); Cork, Carl W. (Walnut Creek, CA); Santarsiero, Bernard D. (Chicago, IL); Stevens, Raymond C. (La Jolla, CA)

    2007-09-25

    A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

  9. Improving network performance on multicore systems: Impact of core affinities on high throughput flows

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenry Bellamy,Impact AssessmentsImproving Reactor

  10. New Challenges of the Computation of Multiple Sequence Alignments in the High-Throughput Era (2010 JGI/ANL HPC Workshop)

    ScienceCinema (OSTI)

    Notredame, Cedric [Centre for Genomic Regulation

    2011-06-08

    Cedric Notredame from the Centre for Genomic Regulation gives a presentation on "New Challenges of the Computation of Multiple Sequence Alignments in the High-Throughput Era" at the JGI/Argonne HPC Workshop on January 26, 2010.

  11. High-throughput metal nanoparticle catalysis by pulsed laser ablation Selim Senkan a,*, Michael Kahn a

    E-Print Network [OSTI]

    Senkan, Selim M.

    -PLA were also screened for their catalytic activities and selectivities for the partial oxidation for catalytic applications. Catalytic materials containing Rh, bimetallic Rh/Pt and trimetallic Rh of propylene. In less than a day, over 40 different catalytic materials of nanoparticles supported on g-Al2O3

  12. Openly Accessible Microfluidic Liquid Handlers for Automated High-Throughput Nanoliter Cell Culture

    E-Print Network [OSTI]

    Huang, Yanyi

    addressability. Moreover, the additional accessories for micropumps and valves, including pressurized gas supply to drug screening2 and genomic profiling.3 These large-scale experiments would naturally prefer minute such as valves, pumps, and necessary bypassing channels, it is still chall- enging to realize individual

  13. A high-throughput behavioral paradigm for Drosophila olfaction -The Flywalk

    E-Print Network [OSTI]

    -delivery system Flywalk. In fifteen aligned small wind tunnels individual flies are exposed to repeated odor

  14. Determination of gene expression patterns using high-throughput RNA in situ hybridizaion to whole-mount Drosophila embryos

    SciTech Connect (OSTI)

    Weiszmann, R.; Hammonds, A.S.; Celniker, S.E.

    2009-04-09

    We describe a high-throughput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in a 96-well format. cDNA or genomic DNA templates are amplified by PCR and then digoxigenin-labeled ribonucleotides are incorporated into antisense RNA probes by in vitro transcription. The quality of each probe is evaluated before ISH using a RNA probe quantification (dot blot) assay. RNA probes are hybridized to fixed, mixed-staged Drosophila embryos in 96-well plates. The resulting stained embryos can be examined and photographed immediately or stored at 4oC for later analysis. Starting with fixed, staged embryos, the protocol takes 6 d from probe template production through hybridization. Preparation of fixed embryos requires a minimum of 2 weeks to collect embryos representing all stages. The method has been used to determine the expression patterns of over 6,000 genes throughout embryogenesis.

  15. High-throughput respirometric assay identifies predictive toxicophore of mitochondrial injury

    SciTech Connect (OSTI)

    Wills, Lauren P. [MitoHealth Inc., Charleston, SC 29403 (United States); Beeson, Gyda C.; Trager, Richard E.; Lindsey, Christopher C. [Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425 (United States); Beeson, Craig C. [MitoHealth Inc., Charleston, SC 29403 (United States); Peterson, Yuri K. [Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425 (United States); Schnellmann, Rick G., E-mail: schnell@musc.edu [Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425 (United States); Ralph H. Johnson VA Medical Center, Charleston, SC 29401 (United States)

    2013-10-15

    Many environmental chemicals and drugs negatively affect human health through deleterious effects on mitochondrial function. Currently there is no chemical library of mitochondrial toxicants, and no reliable methods for predicting mitochondrial toxicity. We hypothesized that discrete toxicophores defined by distinct chemical entities can identify previously unidentified mitochondrial toxicants. We used a respirometric assay to screen 1760 compounds (5 ?M) from the LOPAC and ChemBridge DIVERSet libraries. Thirty-one of the assayed compounds decreased uncoupled respiration, a stress test for mitochondrial dysfunction, prior to a decrease in cell viability and reduced the oxygen consumption rate in isolated mitochondria. The mitochondrial toxicants were grouped by chemical similarity and two clusters containing four compounds each were identified. Cheminformatic analysis of one of the clusters identified previously uncharacterized mitochondrial toxicants from the ChemBridge DIVERSet. This approach will enable the identification of mitochondrial toxicants and advance the prediction of mitochondrial toxicity for both drug discovery and risk assessment. - Highlights: • Respirometric assay conducted in RPTC to create mitochondrial toxicant database. • Chemically similar mitochondrial toxicants aligned as mitochondrial toxicophores • Mitochondrial toxicophore identifies five novel mitochondrial toxicants.

  16. High-throughput identification of off-targets for the mechanistic study of severe adverse drug reactions induced by analgesics

    SciTech Connect (OSTI)

    Pan, Jian-Bo [Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005 (China); Ji, Nan; Pan, Wen; Hong, Ru [State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102 (China); Wang, Hao [Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005 (China); Ji, Zhi-Liang, E-mail: appo@xmu.edu.cn [State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102 (China); Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005 (China)

    2014-01-01

    Drugs may induce adverse drug reactions (ADRs) when they unexpectedly bind to proteins other than their therapeutic targets. Identification of these undesired protein binding partners, called off-targets, can facilitate toxicity assessment in the early stages of drug development. In this study, a computational framework was introduced for the exploration of idiosyncratic mechanisms underlying analgesic-induced severe adverse drug reactions (SADRs). The putative analgesic-target interactions were predicted by performing reverse docking of analgesics or their active metabolites against human/mammal protein structures in a high-throughput manner. Subsequently, bioinformatics analyses were undertaken to identify ADR-associated proteins (ADRAPs) and pathways. Using the pathways and ADRAPs that this analysis identified, the mechanisms of SADRs such as cardiac disorders were explored. For instance, 53 putative ADRAPs and 24 pathways were linked with cardiac disorders, of which 10 ADRAPs were confirmed by previous experiments. Moreover, it was inferred that pathways such as base excision repair, glycolysis/glyconeogenesis, ErbB signaling, calcium signaling, and phosphatidyl inositol signaling likely play pivotal roles in drug-induced cardiac disorders. In conclusion, our framework offers an opportunity to globally understand SADRs at the molecular level, which has been difficult to realize through experiments. It also provides some valuable clues for drug repurposing. - Highlights: • A novel computational framework was developed for mechanistic study of SADRs. • Off-targets of drugs were identified in large scale and in a high-throughput manner. • SADRs like cardiac disorders were systematically explored in molecular networks. • A number of ADR-associated proteins were identified.

  17. High throughput lessons from the LHC experience.Johnston.TNC2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    signal transport, layer 2 transport, data transport (TCP is still the norm), operating system evolution, data movement and management techniques and software, and increasing...

  18. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    have great promise as hydrogen storage materials due tofor regenerative hydrogen storage applications. Addition ofrequired of a viable hydrogen storage system, but further

  19. A microfluidics approach towards high-throughput pathogen removal from blood using margination

    E-Print Network [OSTI]

    Hou, Han Wei

    Sepsis is an adverse systemic inflammatory response caused by microbial infection in blood. This paper reports a simple microfluidic approach for intrinsic, non-specific removal of both microbes and inflammatory cellular ...

  20. LabFlow1: a Database Benchmark for HighThroughput Workflow Management ?

    E-Print Network [OSTI]

    Bonner, Anthony

    work­ flow management systems (WFMS). An important component of many WFMSs is a DBMS for keeping track of workflow activity. This DBMS maintains an audit trail, or event history, that records the results of each evolving business environment. Since the database schema depends on the work­ flow, the DBMS must also

  1. Database Requirements for Workflow Management in a HighThroughput Genome Laboratory 1

    E-Print Network [OSTI]

    Bonner, Anthony

    and operationally efficient. A key component of this software is a database management system (DBMS) for controlling and tracking workflow activity. This DBMS maintains a record of what happened in the laboratory. This record the requirements of this DBMS. The discussion centers on LabFlow­1 [3, 2], a recently developed database benchmark

  2. LabFlow1: a Database Benchmark for HighThroughput Workflow Management \\Lambda

    E-Print Network [OSTI]

    Bonner, Anthony

    called workflow management systems (WFMS). An important component of many WFMSs is a DBMS for keeping track of workflow activity. This DBMS maintains an audit trail, or event history, that records a rapidly evolving business environment. Since the database schema depends on the workflow, the DBMS must

  3. Interleaving techniques for high-throughput chaotic noise generation in CMOS

    E-Print Network [OSTI]

    Sergio Callegari; Riccardo Rovatti; Gianluca Setti

    2015-07-01

    An interleaving technique is proposed to enhance the throughput of current-mode CMOS discrete time chaotic sources based on the iteration of unidimensional maps. A discussion of the reasons and the advantages offered by the approach is provided, together with analytical results about the conservation of some major statistical features. As an example, application to an FM-DCSK communication system is proposed. To conclude, a sample circuit capable of 20 Msample/s is presented.

  4. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control{

    E-Print Network [OSTI]

    Sinskey, Anthony J.

    Microbioreactor arrays with integrated mixers and fluid injectors for high- throughput, with 100 mL working volume, comprising a peristaltic oxygenating mixer and microfluidic injectors litre reference fermentation, conducted with the same strain, in a bench scale stirred tank bioreactor

  5. SPATIALLY-SEPARATED ATOMIC LAYER DEPOSITION OF Al2O3, A NEW OPTION FOR HIGH-THROUGHPUT Si SOLAR CELL PASSIVATION

    E-Print Network [OSTI]

    SPATIALLY-SEPARATED ATOMIC LAYER DEPOSITION OF Al2O3, A NEW OPTION FOR HIGH-THROUGHPUT Si SOLAR material for surface passivation of crystalline Si is atomic layer deposited (ALD) Al2O3. However for the development of high-efficiency solar cells. It has been shown that atomic layer deposition (ALD) of Al2O3

  6. Exploring Flexible Strategies in Engineering Systems Using Screening Models Applications to Offshore Petroleum Projects

    E-Print Network [OSTI]

    de Weck, Olivier L.

    to Offshore Petroleum Projects by Jijun Lin B.E., Mechanical Engineering, Beijing University of Aeronautics Flexible Strategies in Engineering Systems Using Screening Models Applications to Offshore Petroleum, such as offshore petroleum exploration and production systems, generally require a significant amount of capital

  7. FELIX: a High-Throughput Network Approach for Interfacing to Front End Electronics for ATLAS Upgrades

    E-Print Network [OSTI]

    Anderson, John Thomas; The ATLAS collaboration; Boterenbrood, Hendrik; Chen, Hucheng; Chen, Kai; Drake, Gary; Francis, David; Gorini, Benedetto; Lanni, Francesco; Lehmann Miotto, Giovanna; Levinson, Lorne; Narevicius, Julia; Plessl, Christian; Roich, Alexander; Ryu, Soo; Schreuder, Frans Philip; Schumacher, Jorn; Vandelli, Wainer; Vermeulen, Jos; Zhang, Jinlong

    2015-01-01

    The ATLAS experiment at CERN is planning full deployment of a new unified optical link technology for connecting detector front end electronics on the timescale of the LHC Run 4 (2025). It is estimated that roughly 8000 GBT (GigaBit Transceiver) links, with transfer rates up to 10.24~Gbps, will replace existing links used for readout, detector control and distribution of timing and trigger information. A new class of devices will be needed to interface many GBT links to the rest of the trigger, data-acquisition and detector control systems. In this paper FELIX (Front End LInk eXchange) is presented, a PC-based device to route data from and to multiple GBT links via a high-performance general purpose network capable of a total throughput up to O(20 Tbps). FELIX implies architectural changes to the ATLAS data acquisition system, such as the use of industry standard COTS components early in the DAQ chain. Additionally the design and implementation of a FELIX demonstration platform is presented, and hardware and ...

  8. Conversion of forest residues to a methane-rich gas in a high-throughput gasifier

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.; Appelbaum, H.R.; Taylor, D.R.

    1988-05-01

    Research was conducted in a process research unit to develop an entrained bed gasifier which is supplied heat by recirculating a stream of sand between a separate combustion vessel and the gasifier. The char remaining after gasification of the wood provides the fuel for the combustor. The research program was conducted in two phases. In the first phase, a 6 in. I.D. gasifier was used to establish the feasibility of the concept for a wide variety of biomass feeds. The second phase of the program was conducted with a 10 in. I.D. gasifier, and a fully automated feeder system, to evaluate gasifier performance at very high feed rates. The experimental results were used to develop design parameters and detailed energy and material balances for a conceptual plant. A preliminary cost analysis is presented in the report based on the conceptual design. 5 refs., 24 figs., 13 tabs.

  9. Screening for Real Options “In” an Engineering System: A Step Towards Flexible System Development

    E-Print Network [OSTI]

    Bartolomei, Jason E.

    2006-04-07

    The goal of this research is to develop an analytical framework for screening for real options “in” an

  10. On-the-fly machine-learning for high-throughput experiments: search for rare-earth-free permanent magnets

    SciTech Connect (OSTI)

    Kusne, AG; Gao, TR; Mehta, A; Ke, LQ; Nguyen, MC; Ho, KM; Antropov, V; Wang, CZ; Kramer, MJ; Long, C; Takeuchi, I

    2014-09-15

    Advanced materials characterization techniques with ever-growing data acquisition speed and storage capabilities represent a challenge in modern materials science, and new procedures to quickly assess and analyze the data are needed. Machine learning approaches are effective in reducing the complexity of data and rapidly homing in on the underlying trend in multi-dimensional data. Here, we show that by employing an algorithm called the mean shift theory to a large amount of diffraction data in high-throughput experimentation, one can streamline the process of delineating the structural evolution across compositional variations mapped on combinatorial libraries with minimal computational cost. Data collected at a synchrotron beamline are analyzed on the fly, and by integrating experimental data with the inorganic crystal structure database (ICSD), we can substantially enhance the accuracy in classifying the structural phases across ternary phase spaces. We have used this approach to identify a novel magnetic phase with enhanced magnetic anisotropy which is a candidate for rare-earth free permanent magnet.

  11. Basic SCADA systems - from the sensors to the screen

    SciTech Connect (OSTI)

    Merlie, B.

    1995-12-01

    Supervisory Control and Data Acquisition (SCADA) Systems are specialized control systems used to monitor and control facilities which are geographically dispersed. They are commonly used in the gas, oil, electric, and water transmission and distribution industries. SCADA systems differ from other control systems in that they make extensive use of remote communications and are more tolerant to outages of the communications network than a typical control system installation in a plant environment. A basic SCADA system can be broken into five functional parts. These are: (1) Sensors and Actuators; (2) Remote Terminal Units (RTUs); (3) Communications Facilities; (4) Host Computer Systems; and (5) User Interfaces While the fundamental concepts of SCADA systems have changed little in the past 20 years, more sophisticated hardware and software has altered some of the traditional paradigms associated with these control systems. This is particularly true with respect to RTUs, host computer systems, and user interfaces. While this paper will focus on the fundamentals, it will attempt to provide the reader with current trends in the industry where applicable.

  12. BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments

    SciTech Connect (OSTI)

    Round, Adam, E-mail: around@embl.fr; Felisaz, Franck [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Université Grenoble Alpes–EMBL–CNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Fodinger, Lukas; Gobbo, Alexandre [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Huet, Julien [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Université Grenoble Alpes–EMBL–CNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Villard, Cyril [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Blanchet, Clement E., E-mail: around@embl.fr [EMBL c/o DESY, Notkestrasse 85, 22603 Hamburg (Germany); Pernot, Petra; McSweeney, Sean [ESRF, 6 Rue Jules Horowitz, 38000 Grenoble (France); Roessle, Manfred; Svergun, Dmitri I. [EMBL c/o DESY, Notkestrasse 85, 22603 Hamburg (Germany); Cipriani, Florent, E-mail: around@embl.fr [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Université Grenoble Alpes–EMBL–CNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France)

    2015-01-01

    A robotic sample changer for solution X-ray scattering experiments optimized for speed and to use the minimum amount of material has been developed. This system is now in routine use at three high-brilliance European synchrotron sites, each capable of several hundred measurements per day. Small-angle X-ray scattering (SAXS) of macromolecules in solution is in increasing demand by an ever more diverse research community, both academic and industrial. To better serve user needs, and to allow automated and high-throughput operation, a sample changer (BioSAXS Sample Changer) that is able to perform unattended measurements of up to several hundred samples per day has been developed. The Sample Changer is able to handle and expose sample volumes of down to 5 µl with a measurement/cleaning cycle of under 1 min. The samples are stored in standard 96-well plates and the data are collected in a vacuum-mounted capillary with automated positioning of the solution in the X-ray beam. Fast and efficient capillary cleaning avoids cross-contamination and ensures reproducibility of the measurements. Independent temperature control for the well storage and for the measurement capillary allows the samples to be kept cool while still collecting data at physiological temperatures. The Sample Changer has been installed at three major third-generation synchrotrons: on the BM29 beamline at the European Synchrotron Radiation Facility (ESRF), the P12 beamline at the PETRA-III synchrotron (EMBL@PETRA-III) and the I22/B21 beamlines at Diamond Light Source, with the latter being the first commercial unit supplied by Bruker ASC.

  13. Scheduling screening inspections for replaceable and non-replaceable systems 

    E-Print Network [OSTI]

    Aral, Bahadir

    2009-05-15

    This dissertation focuses on developing inspection schedules to detect non-self- announcing events which can only detected by inspections. Failures of protective sys- tems ,such as electronic equipments, alarms and stand-by systems, incipient...

  14. High throughput microcantilever detector

    DOE Patents [OSTI]

    Thundat, Thomas G.; Ferrell, Thomas L.; Hansen, Karolyn M.; Tian, Fang

    2004-07-20

    In an improved uncoated microcantilever detector, the sample sites are placed on a separate semi-conducting substrate and the microcantilever element detects and measures the changes before and after a chemical interaction or hybridization of the sites by sensing differences of phase angle between an alternating voltage applied to the microcantilever element and vibration of the microcantilever element. In another embodiment of the invention, multiple sample sites are on a sample array wherein an array of microcantilever elements detect and measure the change before and after chemical interactions or hybridizations of the sample sites.

  15. High-Performance Binary Protein Interaction Screening in a Microfluidic Format

    E-Print Network [OSTI]

    Quake, Stephen R.

    and Micro-Architecture; Protein-Protein Interaction; High-Throughput Technology Introduction MicrofluidicHigh-Performance Binary Protein Interaction Screening in a Microfluidic Format Matthias Meier1. For this, synergistic effects between the micro- and chemical architecture of the chip are exploited

  16. Stimulators of translation identified during a small molecule screening Unkyung Shin a

    E-Print Network [OSTI]

    Vajda, Sandor

    of natural and synthetic products for eukaryotic translation modulators, we iden- tified two natural products of these compounds led to the insight that mRNA used to program the translation extracts during high-throughput assay identify a source of ``false positives'' for high-through- put screen campaigns using translation extracts

  17. Artificial light and quantum order in systems of screened dipoles Xiao-Gang Wen

    E-Print Network [OSTI]

    Wen, Xiao-Gang

    Artificial light and quantum order in systems of screened dipoles Xiao-Gang Wen Department) The origin of light is a unsolved mystery in nature. Recently, it was suggested that light may originate from magnetic/electric dipoles in 2D and 3D lattices. We show that our models contain an artificial light

  18. In vivo robotics: the automation of neuroscience and other intact-system biological fields

    E-Print Network [OSTI]

    Forest, Craig R.

    Robotic and automation technologies have played a huge role in in vitro biological science, having proved critical for scientific endeavors such as genome sequencing and high-throughput screening. Robotic and automation ...

  19. High energy x-ray diffraction/x-ray fluorescence spectroscopy for high-throughput analysis of composition spread thin films

    SciTech Connect (OSTI)

    Gregoire, John M. [Department of Physics, and Cornell Fuel Cell Institute, Cornell University, Ithaca, New York 14853 (United States); Dale, Darren; Kazimirov, Alexander [Cornell High Energy Synchrotron Source, Cornell University, New York 14853 (United States); DiSalvo, Francis J. [Department of Chemistry and Chemical Biology, and Cornell Fuel Cell Institute, Cornell University, New York 14853 (United States); Dover, R. Bruce van [Department of Materials Science and Engineering, and Cornell Fuel Cell Institute, Cornell University, New York 14853 (United States)

    2009-12-15

    High-throughput crystallography is an important tool in materials research, particularly for the rapid assessment of structure-property relationships. We present a technique for simultaneous acquisition of diffraction images and fluorescence spectra on a continuous composition spread thin film using a 60 keV x-ray source. Subsequent noninteractive data processing provides maps of the diffraction profiles, thin film fiber texture, and composition. Even for highly textured films, our diffraction technique provides detection of diffraction from each family of Bragg reflections, which affords direct comparison of the measured profiles with powder patterns of known phases. These techniques are important for high throughput combinatorial studies as they provide structure and composition maps which may be correlated with performance trends within an inorganic library.

  20. Assessment of advanced coal-gasification processes. [AVCO high throughput gasification in process; Bell High Mass Flux process; CS-R process; and Exxon Gasification process

    SciTech Connect (OSTI)

    McCarthy, J.; Ferrall, J.; Charng, T.; Houseman, J.

    1981-06-01

    This report represents a technical assessment of the following advanced coal gasification processes: AVCO High Throughput Gasification (HTG) Process, Bell Single - Stage High Mass Flux (HMF) Process, Cities Service/Rockwell (CS/R) Hydrogasification Process, and the Exxon Catalytic Coal Gasification (CCG) Process. Each process is evaluated for its potential to produce SNG from a bituminous coal. In addition to identifying the new technology these processes represent, key similarities/differences, strengths/weaknesses, and potential improvements to each process are identified. The AVCO HTG and the Bell HMF gasifiers share similarities with respect to: short residence time (SRT), high throughput rate, slagging and syngas as the initial raw product gas. The CS/R Hydrogasifier is also SRT but is non-slagging and produces a raw gas high in methane content. The Exxon CCG gasifier is a long residence time, catalytic fluidbed reactor producing all of the raw product methane in the gasifier.

  1. Plasma Screen Floating Mount

    DOE Patents [OSTI]

    Eakle, Robert F. (New Ellenton, SC); Pak, Donald J. (Martine, GA)

    2004-10-26

    A mounting system for a flat display screen, particularly a plasma display screen, suspends the screen separately in each of the x-, y- and z-directions. A series of frames located by linear bearings and isolated by springs and dampers allows separate controlled movement in each axis. The system enables the use of relatively larger display screens in vehicles in which plasma screen are subject to damage from vibration.

  2. High-throughput system can analyze thousands of poplar, maize, and switch grass samples at a time to help determine

    E-Print Network [OSTI]

    to help determine which biomass feedstocks are best suited for biofuels. Researchers at the National

  3. Advances in High-Throughput Screening for Drug Discovery The UIC Center for Clinical and Translational Science is sponsoring a translational technology symposium on

    E-Print Network [OSTI]

    Illinois at Chicago, University of

    the Harvard/MIT Broad Institute, Sam Michael, from the NIH Chemical Genomics Center, Annie Mak, from the Genomics Institute of the Novartis Research Foundation, and Marcie Glicksman, from the Harvard Neuro-Scanning-Coupled Microscopy Sam Michael, Group Leader, Automation and Compound Management, NIH Chemical Genomics Center 11

  4. Combinatorial Chemistry & High Throughput Screening, 2010, 13, 67-74 67 1386-2073/10 $55.00+.00 2010 Bentham Science Publishers Ltd.

    E-Print Network [OSTI]

    Senkan, Selim M.

    supported Cr, Mn, Cu, Ru, Pd, Ag, Sn, and Ir as the most promising leads for PO synthesis. Subsequent of Chemical Engineering, METU Ankara, Turkey Abstract: Nanoparticles of 35 individual metals as well catalysis, partial oxidation, laser ablation, nanoparticles, array micro-reactors. 1. INTRODUCTION Propylene

  5. Capillary Flow Layer-by-Layer: A Microfluidic Platform for the High-Throughput Assembly and Screening of Nanolayered Film Libraries

    E-Print Network [OSTI]

    Castleberry, Steven A.

    Layer-by-layer (LbL) assembly is a powerful tool with increasing real world applications in energy, biomaterials, active surfaces, and membranes; however, the current state of the art requires individual sample construction ...

  6. Combinatorial Chemistry & High Throughput Screening, 2001, 4, 661-673 661 AnEvolutionaryApproachfor Structure-based Design of Natural and Non-

    E-Print Network [OSTI]

    Caflisch, Amedeo

    EvolutionaryApproachfor Structure-based Design of Natural and Non- natural Peptidic Ligands Nicolas Budin, Shaheen Ahmed, Nicolas- throughput homology modelling [4] are providing a large amount of targets for structure-based drug design-8057 Zürich, Switzerland Abstract: A new computational approach (PEP) is presented for the structure

  7. Combinatorial Chemistry & High Throughput Screening, 2012, 15, 595-610 595 1875-5402/12 $58.00+.00 2012 Bentham Science Publishers

    E-Print Network [OSTI]

    Powers, Robert

    and heat treatments. Similarly, in the late 18th century, the chemical analysis of urine was able]. Thus, early medical treatments and diagnosis were based on attempts to understand these humors. Before

  8. Development of Quantitative FRET Technology for SENP Enzyme Kinetics Determinations and High-Sensitive High-Throughput Screening Assay for Protease Inhibitor Discovery

    E-Print Network [OSTI]

    Liu, Yan

    2012-01-01

    exponential increased reaction velocity. Data were analyzedEq. 13] The initial reaction velocity is the initial linearexponential increased reaction velocity. Data were analyzed

  9. Definition, Expansion and Screening of Architectures for Planetary Exploration Class Nuclear Electric Propulsion and Power Systems

    E-Print Network [OSTI]

    Definition, Expansion and Screening of Architectures for Planetary Exploration Class Nuclear, expansion and screening of Nuclear Electric Propulsion and Power concepts capable of achieving planetary left blank 2 #12;Definition, Expansion and Screening of Architectures for Planetary Exploration Class

  10. Comparative performance of modern digital mammography systems in a large breast screening program

    SciTech Connect (OSTI)

    Yaffe, Martin J. Bloomquist, Aili K.; Hunter, David M.; Mawdsley, Gordon E.; Chiarelli, Anna M.; Muradali, Derek; Mainprize, James G.

    2013-12-15

    Purpose: To compare physical measures pertaining to image quality among digital mammography systems utilized in a large breast screening program. To examine qualitatively differences in these measures and differences in clinical cancer detection rates between CR and DR among sites within that program. Methods: As part of the routine quality assurance program for screening, field measurements are made of several variables considered to correlate with the diagnostic quality of medical images including: modulation transfer function, noise equivalent quanta, d? (an index of lesion detectability) and air kerma to allow estimation of mean glandular dose. In addition, images of the mammography accreditation phantom are evaluated. Results: It was found that overall there were marked differences between the performance measures of DR and CR mammography systems. In particular, the modulation transfer functions obtained with the DR systems were found to be higher, even for larger detector element sizes. Similarly, the noise equivalent quanta, d?, and the phantom scores were higher, while the failure rates associated with low signal-to-noise ratio and high dose were lower with DR. These results were consistent with previous findings in the authors’ program that the breast cancer detection rates at sites employing CR technology were, on average, 30.6% lower than those that used DR mammography. Conclusions: While the clinical study was not large enough to allow a statistically powered system-by-system assessment of cancer detection accuracy, the physical measures expressing spatial resolution, and signal-to-noise ratio are consistent with the published finding that sites employing CR systems had lower cancer detection rates than those using DR systems for screening mammography.

  11. Apparatus for combinatorial screening of electrochemical materials

    DOE Patents [OSTI]

    A high throughput combinatorial screening method and apparatus for the evaluation of electrochemical materials using a single voltage source (2) is disclosed wherein temperature changes arising from the application of an electrical load to a cell array (1) are used to evaluate the relative electrochemical efficiency of the materials comprising the array. The apparatus may include an array of electrochemical cells (1) that are connected to each other in parallel or in series, an electronic load (2) for applying a voltage or current to the electrochemical cells (1), and a device (3), external to the cells, for monitoring the relative temperature of each cell when the load is applied.

    2009-12-15

    A high throughput combinatorial screening method and apparatus for the evaluation of electrochemical materials using a single voltage source (2) is disclosed wherein temperature changes arising from the application of an electrical load to a cell array (1) are used to evaluate the relative electrochemical efficiency of the materials comprising the array. The apparatus may include an array of electrochemical cells (1) that are connected to each other in parallel or in series, an electronic load (2) for applying a voltage or current to the electrochemical cells (1), and a device (3), external to the cells, for monitoring the relative temperature of each cell when the load is applied.

  12. Investigating the Operational Capabilities of Custom and Pedestrian Portal Monitoring Systems for Screening Livestock for Radioactive Contamination 

    E-Print Network [OSTI]

    Erchinger, Jennifer

    2013-05-01

    in livestock screening due to sensitive alarm algorithms, greater position discrimination with four detectors, and ease of adjustment for agricultural purposes. The last phase of this project included designing and constructing a portal system that included...

  13. Neurodegenerative diseases: Lessons from genome-wide screens in small model organisms Molecular mechanisms of aging-related neurodegenerative diseases: emerging views

    E-Print Network [OSTI]

    Breitling, Rainer

    , including Parkinson's disease, polyglutamine expansion diseases and Alzheimer's disease, are associated used to model these diseases and high throughput genetic screens using these models have led-analysis #12;3 Introduction Several age-related neurodegenerative diseases, including Parkinson's disease

  14. Prediction of rodent carcinogenic potential of naturally occurring chemicals in the human diet using high-throughput QSAR predictive modeling

    SciTech Connect (OSTI)

    Valerio, Luis G. . E-mail: luis.valerio@FDA.HHS.gov; Arvidson, Kirk B.; Chanderbhan, Ronald F.; Contrera, Joseph F.

    2007-07-01

    Consistent with the U.S. Food and Drug Administration (FDA) Critical Path Initiative, predictive toxicology software programs employing quantitative structure-activity relationship (QSAR) models are currently under evaluation for regulatory risk assessment and scientific decision support for highly sensitive endpoints such as carcinogenicity, mutagenicity and reproductive toxicity. At the FDA's Center for Food Safety and Applied Nutrition's Office of Food Additive Safety and the Center for Drug Evaluation and Research's Informatics and Computational Safety Analysis Staff (ICSAS), the use of computational SAR tools for both qualitative and quantitative risk assessment applications are being developed and evaluated. One tool of current interest is MDL-QSAR predictive discriminant analysis modeling of rodent carcinogenicity, which has been previously evaluated for pharmaceutical applications by the FDA ICSAS. The study described in this paper aims to evaluate the utility of this software to estimate the carcinogenic potential of small, organic, naturally occurring chemicals found in the human diet. In addition, a group of 19 known synthetic dietary constituents that were positive in rodent carcinogenicity studies served as a control group. In the test group of naturally occurring chemicals, 101 were found to be suitable for predictive modeling using this software's discriminant analysis modeling approach. Predictions performed on these compounds were compared to published experimental evidence of each compound's carcinogenic potential. Experimental evidence included relevant toxicological studies such as rodent cancer bioassays, rodent anti-carcinogenicity studies, genotoxic studies, and the presence of chemical structural alerts. Statistical indices of predictive performance were calculated to assess the utility of the predictive modeling method. Results revealed good predictive performance using this software's rodent carcinogenicity module of over 1200 chemicals, comprised primarily of pharmaceutical, industrial and some natural products developed under an FDA-MDL cooperative research and development agreement (CRADA). The predictive performance for this group of dietary natural products and the control group was 97% sensitivity and 80% concordance. Specificity was marginal at 53%. This study finds that the in silico QSAR analysis employing this software's rodent carcinogenicity database is capable of identifying the rodent carcinogenic potential of naturally occurring organic molecules found in the human diet with a high degree of sensitivity. It is the first study to demonstrate successful QSAR predictive modeling of naturally occurring carcinogens found in the human diet using an external validation test. Further test validation of this software and expansion of the training data set for dietary chemicals will help to support the future use of such QSAR methods for screening and prioritizing the risk of dietary chemicals when actual animal data are inadequate, equivocal, or absent.

  15. High-throughput on-chip in vivo neural regeneration studies usingfemtosecond laser nano-surgery and microfluidics

    E-Print Network [OSTI]

    Samara, Chrysanthi

    In recent years, the advantages of using small invertebrate animals as model systems for human disease have become increasingly apparent and have resulted in three Nobel Prizes in medicine or chemistry during the last six ...

  16. Function Prediction for Hypothetical Proteins in Yeast Saccharomyces cerevisiae Using Multiple Sources of High-Throughput Data

    E-Print Network [OSTI]

    , we have developed GeneFAS (Gene Function Annotation System) using an integrated probabilistic method Address: Digital Biology Laboratory, Computer Science Department, 201 Engineering Building West of predicting functions of unannotated proteins by developing efficient and automated methods. Several

  17. Use of Time-Aggregated Data in Economic Screening Analyses of Combined Heat and Power Systems

    SciTech Connect (OSTI)

    Hudson II, Carl Randy

    2004-09-01

    Combined heat and power (CHP) projects (also known as cogeneration projects) usually undergo a series of assessments and viability checks before any commitment is made. A screening analysis, with electrical and thermal loads characterized on an annual basis, may be performed initially to quickly determine the economic viability of the proposed project. Screening analyses using time-aggregated data do not reflect several critical cost influences, however. Seasonal and diurnal variations in electrical and thermal loads, as well as time-of-use utility pricing structures, can have a dramatic impact on the economics. A more accurate economic assessment requires additional detailed data on electrical and thermal demand (e.g., hourly load data), which may not be readily available for the specific facility under study. Recent developments in CHP evaluation tools, however, can generate the needed hourly data through the use of historical data libraries and building simulation. This article utilizes model-generated hourly load data for four potential CHP applications and compares the calculated cost savings of a CHP system when evaluated on a time-aggregated (i.e., annual) basis to the savings when evaluated on an hour-by-hour basis. It is observed that the simple, aggregated analysis forecasts much greater savings (i.e., greater economic viability) than the more detailed hourly analysis. The findings confirm that the simpler tool produces results with a much more optimistic outlook, which, if taken by itself, might lead to erroneous project decisions. The more rigorous approach, being more reflective of actual requirements and conditions, presents a more accurate economic comparison of the alternatives, which, in turn, leads to better decision risk management.

  18. Cluster Computing: High-Performance, High-Availability, and High-Throughput Processing on a Network of Computers

    E-Print Network [OSTI]

    Buyya, Rajkumar

    Laboratory Dept. of Computer Science and Software Engineering The University of Melbourne, Australia {csyeo, raj}@cs.mu.oz.au 2 Parallel and Distributed Systems Laboratory Department of Computer Science for cluster computing was developed in the 1960s by IBM as an alternative of linking large mainframes

  19. Exploration, Registration, and Analysis of High-Throughput 3D Microscopy Data from the Knife-Edge Scanning Microscope 

    E-Print Network [OSTI]

    Sung, Chul

    2014-04-25

    resolution methods like diffusion MRI [8, 27, 76, 94] on the one hand and small- scale, higher resolution methods like SBF-SEM [18] on the other hand. So, mapping our KESM data sets to a standard coordinate system for the mouse brain provides deeper... . . . . . . . . . . . . . . . . . . . . . . . . 71 6.1.5 Data Transmission and Visualization . . . . . . . . . . . . . . 71 6.2 Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 6.3 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 7...

  20. High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53 - NF-kappaB - complex-dependent gene expression in human heart failure

    E-Print Network [OSTI]

    Choy, Mun-Kit; Movassagh, Mehregan; Siggens, Lee; Vujic, Ana; Goddard, Martin; Sanchez, Ana; Perkins, Neil; Figg, Nichola; Bennett, Martin; Carroll, Jason; Foo, Roger S.-Y.

    2010-06-14

    with this cis-element. Using high-throughput sequencing, we analyzed genome-wide binding sites for the p53-RELA complex in diseased and control human hearts and found a significant overrepresentation of the STAT3 motif. We further determined that STAT3...

  1. Conversion of forest residues to a methane-rich gas in a high-throughput gasifier. Summary report

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.; Folsom, D.W.; Kim, B.C.

    1981-10-31

    Results of the experimental work conducted thus far have shown that wood can be readily gasified in a steam environment into a hydrocarbon rich fuel gas that can be used as a replacement for petroleum-based fuels or natural gas with minimal boiler retrofit. Further, this conversion can be achieved in a compact gasification reactor with heat supplied by a circulating entrained phase, thereby eliminating the need for an oxygen plant. Tars have not been found except at the lowest gasifier temperatures employed, and therefore heat recovery from the product gas should be much simpler than that from commercially available fixed-bed gasification systems where product gas contains significant quantities of tar. The data generated have been used in a preliminary conceptual design. Evaluation of this design has shown that a medium-Btu gas can be produced from wood at a cost competitive with natural gas or petroleum-based fuels.

  2. High-throughput behavioral phenotyping of drug and alcohol susceptibility traits in the expanded panel of BXD recombinant inbred strains

    SciTech Connect (OSTI)

    Philip, Vivek M [ORNL; Ansah, T [University of Tennessee Health Science Center, Memphis; Blaha, C, [University of Tennessee Health Science Center, Memphis; Cook, Melloni N. [University of Memphis; Hamre, Kristin M. [University of Tennessee Health Science Center, Memphis; Lariviere, William R [University of Pittsburgh; Matthews, Douglas B [Baylor University; Goldowitz, Daniel [University of British Columbia, Vancouver; Chesler, Elissa J [ORNL

    2010-01-01

    Genetic reference populations, particularly the BXD recombinant inbred strains, are a valuable resource for the discovery of the bio-molecular substrates and genetic drivers responsible for trait variation and co- ariation. This approach can be profitably applied in the analysis of susceptibility and mechanisms of drug and alcohol use disorders for which many predisposing behaviors may predict occurrence and manifestation of increased preference for these substances. Many of these traits are modeled by common mouse behavioral assays, facilitating the detection of patterns and sources of genetic co-regulation of predisposing phenotypes and substance consumption. Members of the Tennessee Mouse Genome Consortium have obtained behavioral phenotype data from 260 measures related to multiple behavioral assays across several domains: self-administration, response to, and withdrawal from cocaine, MDMA, morphine and alcohol; novelty seeking; behavioral despair and related neurological phenomena; pain sensitivity; stress sensitivity; anxiety; hyperactivity; and sleep/wake cycles. All traits have been measured in both sexes and the recently expanded panel of 69 additional BXD recombinant inbred strains (N=69). Sex differences and heritability estimates were obtained for each trait, and a comparison of early (N = 32) and recent BXD RI lines was performed. Primary data is publicly available for heritability, sex difference and genetic analyses using www.GeneNetwork.org. These analyses include QTL detection and genetic analysis of gene expression. Stored results from these analyses are available at http://ontologicaldiscovery.org for comparison to other genomic analysis results. Together with the results of related studies, these data form a public resource for integrative systems genetic analysis of neurobehavioral traits.

  3. HIV-1 entry inhibition by small-molecule CCR5 antagonists: A combined molecular modeling and mutant study using a high-throughput assay

    SciTech Connect (OSTI)

    Labrecque, Jean; Metz, Markus; Lau, Gloria; Darkes, Marilyn C.; Wong, Rebecca S.Y.; Bogucki, David; Carpenter, Bryon; Chen Gang; Li Tongshuang; Nan, Susan; Schols, Dominique; Bridger, Gary J.; Fricker, Simon P.; Skerlj, Renato T.

    2011-05-10

    Based on the attrition rate of CCR5 small molecule antagonists in the clinic the discovery and development of next generation antagonists with an improved pharmacology and safety profile is necessary. Herein, we describe a combined molecular modeling, CCR5-mediated cell fusion, and receptor site-directed mutagenesis approach to study the molecular interactions of six structurally diverse compounds (aplaviroc, maraviroc, vicriviroc, TAK-779, SCH-C and a benzyloxycarbonyl-aminopiperidin-1-yl-butane derivative) with CCR5, a coreceptor for CCR5-tropic HIV-1 strains. This is the first study using an antifusogenic assay, a model of the interaction of the gp120 envelope protein with CCR5. This assay avoids the use of radioactivity and HIV infection assays, and can be used in a high throughput mode. The assay was validated by comparison with other established CCR5 assays. Given the hydrophobic nature of the binding pocket several binding models are suggested which could prove useful in the rational drug design of new lead compounds.

  4. High-Throughput Plasmonic Nanolithography

    E-Print Network [OSTI]

    Pan, Liang

    2010-01-01

    60 4.2.5 Lubricant and Overcoats63 Figure 4.10: Scheme of lubricant durability65 Figure 4.11: Three lubricant flying tests at different

  5. High-Throughput Plasmonic Nanolithography

    E-Print Network [OSTI]

    Pan, Liang

    2010-01-01

    heating Module..FH Control and Resist Pre-heating Module Inspired by thecontrol and resist pre-heating module using the same laser

  6. Biochemical & Thermochemical High Throughput Characterization...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    20 40 60 80 100 120 140 32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62 64 66 68 70 Frequency Corn Stover Corn Cob Miscanthus Wheat...

  7. Composite scintillator screen

    DOE Patents [OSTI]

    Zeman, Herbert D. (1687 Peach St., Memphis, TN 38112)

    1994-01-01

    A scintillator screen for an X-ray system includes a substrate of low-Z material and bodies of a high-Z material embedded within the substrate. By preselecting the size of the bodies embedded within the substrate, the spacial separation of the bodies and the thickness of the screen, the sensitivity of the screen to X-rays within a predetermined energy range can be predicted.

  8. Project Profile: High Operating Temperature Liquid Metal Heat...

    Office of Environmental Management (EM)

    multi-target co-sputtering system to create massive compositional libraries in thin-film forms and employ high-throughput characterization methods to rapidly screen candidate...

  9. From screen to structure with a harvestable microfluidic device

    SciTech Connect (OSTI)

    Stojanoff V.; Jakonic, J.; Oren, D.A.; Nagarajan, V.; Navarro Poulsen, J.C.; Adams-Cioaba, M.A.; Bergfors, T. and Sommer, M.O.A.

    2011-06-21

    Advances in automation have facilitated the widespread adoption of high-throughput vapor-diffusion methods for initial crystallization screening. However, for many proteins, screening thousands of crystallization conditions fails to yield crystals of sufficient quality for structural characterization. Here, the rates of crystal identification for thaumatin, catalase and myoglobin using microfluidic Crystal Former devices and sitting-drop vapour-diffusion plates are compared. It is shown that the Crystal Former results in a greater number of identified initial crystallization conditions compared with vapor diffusion. Furthermore, crystals of thaumatin and lysozyme obtained in the Crystal Former were used directly for structure determination both in situ and upon harvesting and cryocooling. On the basis of these results, a crystallization strategy is proposed that uses multiple methods with distinct kinetic trajectories through the protein phase diagram to increase the output of crystallization pipelines.

  10. Plant Phenotype Characterization System

    SciTech Connect (OSTI)

    Daniel W McDonald; Ronald B Michaels

    2005-09-09

    This report is the final scientific report for the DOE Inventions and Innovations Project: Plant Phenotype Characterization System, DE-FG36-04GO14334. The period of performance was September 30, 2004 through July 15, 2005. The project objective is to demonstrate the viability of a new scientific instrument concept for the study of plant root systems. The root systems of plants are thought to be important in plant yield and thus important to DOE goals in renewable energy sources. The scientific study and understanding of plant root systems is hampered by the difficulty in observing root activity and the inadequacy of existing root study instrumentation options. We have demonstrated a high throughput, non-invasive, high resolution technique for visualizing plant root systems in-situ. Our approach is based upon low-energy x-ray radiography and the use of containers and substrates (artificial soil) which are virtually transparent to x-rays. The system allows us to germinate and grow plant specimens in our containers and substrates and to generate x-ray images of the developing root system over time. The same plant can be imaged at different times in its development. The system can be used for root studies in plant physiology, plant morphology, plant breeding, plant functional genomics and plant genotype screening.

  11. Preliminary Screening

    Office of Energy Efficiency and Renewable Energy (EERE)

    The first step in assessing renewable energy options is to conduct a preliminary screening to decide which technologies are worth investigating and which can be eliminated immediately. Preliminary...

  12. Screening Analysis for the Environmental Risk Evaluation System Fiscal Year 2011 Report Environmental Effects of Offshore Wind Energy

    SciTech Connect (OSTI)

    Copping, Andrea E.; Hanna, Luke A.

    2011-11-01

    Potential environmental effects of offshore wind (OSW) energy development are not well understood, and yet regulatory agencies are required to make decisions in spite of substantial uncertainty about environmental impacts and their long-term consequences. An understanding of risks associated with interactions between OSW installations and avian and aquatic receptors, including animals, habitats, and ecosystems, can help define key uncertainties and focus regulatory actions and scientific studies on interactions of most concern. During FY 2011, Pacific Northwest National Laboratory (PNNL) scientists adapted and applied the Environmental Risk Evaluation System (ERES), first developed to examine the effects of marine and hydrokinetic energy devices on aquatic environments, to offshore wind development. PNNL scientists conducted a risk screening analysis on two initial OSW cases: a wind project in Lake Erie and a wind project off the Atlantic coast of the United States near Atlantic City, New Jersey. The screening analysis revealed that top-tier stressors in the two OSW cases were the dynamic effects of the device (e.g., strike), accidents/disasters, and effects of the static physical presence of the device, such as alterations in bottom habitats. Receptor interactions with these stressors at the highest tiers of risk were dominated by threatened and endangered animals. Risk to the physical environment from changes in flow regime also ranked high. Peer review of this process and results will be conducted during FY 2012. The ERES screening analysis provides an assessment of the vulnerability of environmental receptors to stressors associated with OSW installations; a probability analysis is needed to determine specific risk levels to receptors. As more data become available that document effects of offshore wind farms on specific receptors in U.S. coastal and Great Lakes waters, probability analyses will be performed.

  13. Co-hydrolysis of hydrothermal and dilute acid pretreated Populus slurries to support development of a high-throughput pretreatment system

    E-Print Network [OSTI]

    Studer, Michael H; Brethauer, Simone; DeMartini, Jaclyn D; McKenzie, Heather L; Wyman, Charles E

    2011-01-01

    process samples. National Renewable Energy Laboratory, NREL/at the National Renewable Energy Laboratory for approxi-the standard National Renewable Energy Laboratory method [

  14. Exploring flexible strategies in engineering systems using screening models : applications to offshore petroleum projects

    E-Print Network [OSTI]

    Lin, Jijun, Ph. D. Massachusetts Institute of Technology

    2009-01-01

    Engineering Systems, such as offshore petroleum exploration and production systems, generally require a significant amount of capital investment under various technical and market uncertainties. Choosing appropriate designs ...

  15. Combinatorial Screening of Advanced Scintillators for High Resolution X-ray Detectors

    SciTech Connect (OSTI)

    Cheng, Shifan; Tao, Dejie; Lynch, Michael; Yuan, Xianglong; Li, Yiqun

    2008-05-12

    The lack of efficient scintillators is a major problem for developing powerful x-ray detectors that are widely used in homeland security, industrial and scientific research. Intematix has developed and applied a high throughput screening process and corresponding crystal growth technology to significantly speed up the discovery process for new efficient scintillators. As a result, Intematix has invented and fabricated three new scintillators both in powder and bulk forms, which possess promising properties such as better radiation hardness and better matching for silicon diode.

  16. System, device, and methods for real-time screening of live cells, biomarkers, and chemical signatures

    DOE Patents [OSTI]

    Sundaram, S Kamakshi [Richland, WA; Riley, Brian J [West Richland, WA; Weber, Thomas J [Richland, WA; Sacksteder, Colette A [West Richland, WA; Addleman, R Shane [Benton City, WA

    2011-06-07

    An ATR-FTIR device and system are described that defect live-cell responses to stimuli and perturbations in real-time. The system and device can monitor perturbations resulting from exposures to various physical, chemical, and biological materials in real-time, as well as those sustained over a long period of time, including those associated with stimuli having unknown modes-of-action (e.g. nanoparticles). The device and system can also be used to identify specific chemical species or substances that profile cellular responses to these perturbations.

  17. RNAi High Throughput Screenings Facilitate the Identification of Proteins Necessary for TCDD-induced CYP1A1 Enzymatic Activity and Aid in the Discovery of a Novel Role for Sin3A in AHR-Mediated Gene Expression

    E-Print Network [OSTI]

    Solaimani, Parrisa Sherry

    2012-01-01

    tetrachlorodibenzi-p-dioxin (TCDD) in liver: comparison ofTetrachlorodibenzo-p-dioxin. Chem. Res. Toxicol. Ishizuka,2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol. Sci. 117 (2),

  18. RNAi High Throughput Screenings Facilitate the Identification of Proteins Necessary for TCDD-induced CYP1A1 Enzymatic Activity and Aid in the Discovery of a Novel Role for Sin3A in AHR-Mediated Gene Expression

    E-Print Network [OSTI]

    Solaimani, Parrisa Sherry

    2012-01-01

    Phenol-red free media reduces light scattering and background fluorescence, whereas dicumarol prevents further metabolic degradationPhenol-red free media reduces light scattering and background fluorescence, whereas dicumarol prevents further metabolic degradation

  19. Bench-scale system design to screen catalyst-coated fabric filters for simultaneous NO{sub x} and particulate control

    SciTech Connect (OSTI)

    Ness, S.R.; Ludlow, D.K. [Univ. of North Dakota, Grand Forks, ND (United States)

    1995-12-31

    A bench-scale system was constructed at the Energy and Environmental Research Center (EERC) to screen performance of various types of V/Ti catalyst-coated, high-temperature, fabric filters manufactured at Owens-Corning, Inc. (OCF). This product is targeted for use in utility and industrial facilities for simultaneous NO{sub x} and particulate control. Described in this paper are the bench-scale system design, operation, and validation of the data produced. 9 refs., 3 figs.

  20. System and method for generating and/or screening potential metal-organic frameworks

    DOE Patents [OSTI]

    Wilmer, Christopher E; Leaf, Michael; Snurr, Randall Q; Farha, Omar K; Hupp, Joseph T

    2015-04-21

    A system and method for systematically generating potential metal-organic framework (MOFs) structures given an input library of building blocks is provided herein. One or more material properties of the potential MOFs are evaluated using computational simulations. A range of material properties (surface area, pore volume, pore size distribution, powder x-ray diffraction pattern, methane adsorption capability, and the like) can be estimated, and in doing so, illuminate unidentified structure-property relationships that may only have been recognized by taking a global view of MOF structures. In addition to identifying structure-property relationships, this systematic approach to identify the MOFs of interest is used to identify one or more MOFs that may be useful for high pressure methane storage.

  1. System and method for generating and/or screening potential metal-organic frameworks

    DOE Patents [OSTI]

    Wilmer, Christopher E; Leaf, Michael; Snurr, Randall Q; Farha, Omar K; Hupp, Joseph T

    2014-12-02

    A system and method for systematically generating potential metal-organic framework (MOFs) structures given an input library of building blocks is provided herein. One or more material properties of the potential MOFs are evaluated using computational simulations. A range of material properties (surface area, pore volume, pore size distribution, powder x-ray diffraction pattern, methane adsorption capability, and the like) can be estimated, and in doing so, illuminate unidentified structure-property relationships that may only have been recognized by taking a global view of MOF structures. In addition to identifying structure-property relationships, this systematic approach to identify the MOFs of interest is used to identify one or more MOFs that may be useful for high pressure methane storage.

  2. Projection screen having reduced ambient light scattering

    DOE Patents [OSTI]

    Sweatt, William C. (Albuquerque, NM)

    2010-05-11

    An apparatus and method for improving the contrast between incident projected light and ambient light reflected from a projection screen are described. The efficiency of the projection screen for reflection of the projected light remains high, while permitting the projection screen to be utilized in a brightly lighted room. Light power requirements from the projection system utilized may be reduced.

  3. High-throughput manufacturing of thin-film CdS/CdTe photovoltaic modules. Annual subcontract report, 16 September 1996--15 January 1998

    SciTech Connect (OSTI)

    Sandwisch, D.W. [Solar Cells, Inc., Toledo, OH (United States)

    1998-08-01

    Cadmium telluride (CdTe) is recognized as one of the leading materials for low-cost photovoltaic modules. Solar Cells, Inc., has developed this technology and is scaling its pilot production capabilities to a multi-megawatt level. The Photovoltaic Manufacturing Technology (PVMaT) subcontract supports these efforts. Activities during the third phase of the program concentrated on process development, equipment design and testing, quality assurance, ES and H programs, and large-scale next-generation coating-system prototype development. These efforts broadly addressed the issues of the manufacturing process for producing thin-film, monolithic CdS/CdTe photovoltaic modules.

  4. Wafer screening device and methods for wafer screening

    DOE Patents [OSTI]

    Sopori, Bhushan; Rupnowski, Przemyslaw

    2014-07-15

    Wafer breakage is a serious problem in the photovoltaic industry because a large fraction of wafers (between 5 and 10%) break during solar cell/module fabrication. The major cause of this excessive wafer breakage is that these wafers have residual microcracks--microcracks that were not completely etched. Additional propensity for breakage is caused by texture etching and incomplete edge grinding. To eliminate the cost of processing the wafers that break, it is best to remove them prior to cell fabrication. Some attempts have been made to develop optical techniques to detect microcracks. Unfortunately, it is very difficult to detect microcracks that are embedded within the roughness/texture of the wafers. Furthermore, even if such detection is successful, it is not straightforward to relate them to wafer breakage. We believe that the best way to isolate the wafers with fatal microcracks is to apply a stress to wafers--a stress that mimics the highest stress during cell/module processing. If a wafer survives this stress, it has a high probability of surviving without breakage during cell/module fabrication. Based on this, we have developed a high throughput, noncontact method for applying a predetermined stress to a wafer. The wafers are carried on a belt through a chamber that illuminates the wafer with an intense light of a predetermined intensity distribution that can be varied by changing the power to the light source. As the wafers move under the light source, each wafer undergoes a dynamic temperature profile that produces a preset elastic stress. If this stress exceeds the wafer strength, the wafer will break. The broken wafers are separated early, eliminating cost of processing into cell/module. We will describe details of the system and show comparison of breakage statistics with the breakage on a production line.

  5. Automated macromolecular crystallization screening

    DOE Patents [OSTI]

    Segelke, Brent W.; Rupp, Bernhard; Krupka, Heike I.

    2005-03-01

    An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.

  6. High-ThroughputTagging of Arabidopsis Proteins

    E-Print Network [OSTI]

    Citovsky, Vitaly

    ­0124 (N.C., G.D., N.V.R.); and The Arabidopsis Information Resource (S.L., S.Y.R.) and Carnegie Institution of Washington (S.L., D.E., S.Y.R), Stanford, California 94305 We developed a high their chromogenic reac- tion products (Taylor, 1997). The use of antigenic tags, such as the T7 or HA epitopes

  7. A toolkit for high-throughput, cross-

    E-Print Network [OSTI]

    Cai, Long

    , Mihail Sarov, Sylke Winkler, Kamil A Lipinski & Pavel Tomancak We generated two complementary genomic. pseudoobscurab 88% 12% 0.12% 8.25% 3.65% Mapped clones (15,204 D.mel / 1,924 D.pse) Sequencing failures (1,426 D.mel / 142 D.pse) BLAST failures (20 D.mel / 1 D.pse) Chimeric clones (530 D.mel / 525 D.pse) 74% 26% 0.04% 5

  8. High Throughput Materials Characterization John M. Gregoire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenry Bellamy, Ph.D. Title: Professor -|

  9. Screening Tool for Desiccant Dehumidification Applications 

    E-Print Network [OSTI]

    Czachorski, M.; Worek, W. M.

    1998-01-01

    (from a library of typical systems) and compares performance of a conventional system to an alternative electric system supplemented with a commercially available desiccant dehumidifier providing the required air-conditioning performance. The screening...

  10. DfX for Nanoelectronic Embedded Systems

    E-Print Network [OSTI]

    Mohanty, Saraju P.

    /18/2013 Nvidia GK110 has 7.1 billion transistors of a 28nm technology. Source: http://www.tomshardware.com/news/nvidia-tesla Systems: Common Story Smarter ... Faster ... High Throughput ... Power Hungry !! Battery Hungry !! 12/18/2013 25 #12;Battery Dependency: Not Overstated Boeing 787's across the globe were grounded in early 2013

  11. Erkitinib, a novel EGFR tyrosine kinase inhibitor screened using a ProteoChip system from a phytochemical library

    SciTech Connect (OSTI)

    Kim, Eung-Yoon; Choi, Young-Jin [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of) [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of); Innopharmascreen, Inc., Asan 336-795 (Korea, Republic of); Park, Chan-Won [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of) [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of); Dept. of Biological Science, Hoseo University, Asan 336-795 (Korea, Republic of); Kang, In-Cheol, E-mail: ickang@hoseo.edu [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of) [Biochip Research Center, Hoseo University, Asan 336-795 (Korea, Republic of); Dept. of Biological Science, Hoseo University, Asan 336-795 (Korea, Republic of); Innopharmascreen, Inc., Asan 336-795 (Korea, Republic of)

    2009-11-20

    Receptor tyrosine kinases (PTKs) play key roles in the pathogenesis of numerous human diseases, including cancer. Therefore PTK inhibitors are currently under intensive investigation as potential drug candidates. Herein, we report on a ProteoChip-based screening of an epidermal growth factor receptor (EGFR) tyrosine kinase (TK) inhibitor, Erkitinibs, from phytochemical libraries. PLC-{gamma}-1 was used as a substrate immobilized on a ProteoChip and incubated with an EGFR kinase to phosphorylate tyrosine residues of the substrate, followed by a fluorescence detection of the substrate recognized by a phospho-specific monoclonal antibody. Erkitinibs inhibited HeLa cell proliferation in a dose-dependent manner. In conclusion, these data suggest that Erkitinibs can be a specific inhibitor of an EGFR kinase and can be further developed as a potent anti-tumor agent.

  12. Electrostatic screening by semiconductors 

    E-Print Network [OSTI]

    Krcmar, Maja

    1998-01-01

    distributions. We determine the sluice screening length for the screening of a charged surface defect, and the interaction energy between two charged surface defects. We find the spatial scales over which dielectric and metallic properties of the semiconductors...

  13. Screening of Hydrocarbon Sources in JET

    SciTech Connect (OSTI)

    J.D. Strachan; W. Fundamenski; M. Charlet; K. Erents; J. Gafert; C. Giroud; M. von Hellermann; G. Matthews; G. McCracken; V. Philipps; J. Spence; M.F. Stamp; K-D. Zastrow; and EFDA-JET Work Programme Collaborators

    2001-06-15

    Carbon is the principal impurity in the Joint European Torus (JET). Methane screening experiments quantify the ability of the scrape-off layer (SOL)/divertor system to ionize carbon and transport it to the divertor, preventing core plasma contamination. Previous JET publications studied edge-localized-mode-averaged high-confinement mode screening, and separately, evaluated the methodology of low-confinement mode (L-mode) screening measurements. This paper extends the L-mode measurements to include relevant plasma parameter scans and DIVIMP modeling of the L-mode screening.

  14. The BioASP Library: ASP Solutions for Systems Biology

    E-Print Network [OSTI]

    Schaub, Torsten

    The BioASP Library: ASP Solutions for Systems Biology Martin Gebser, Arne K¨onig, Torsten Schaub--Today's molecular biology is confronted with enor- mous amounts of data, generated by new high-throughput tech- nologies, along with an increasing number of biological models available over web repositories. This poses

  15. DfX for Nanoelectronic Circuits and Systems

    E-Print Network [OSTI]

    Mohanty, Saraju P.

    /23/2013 Nvidia GK110 has 7.1 billion transistors of a 28nm technology. Source: http://www.tomshardware.com/news/nvidia-tesla Systems: Common Story Smarter ... Faster ... High Throughput ... Power Hungry !! Battery Hungry !! 12/23/2013 25 #12;Battery Dependency: Not Overstated Boeing 787's across the globe were grounded in early 2013

  16. An hourly-based performance comparison of an integrated micro-structural perforated shading screen with standard shading systems

    E-Print Network [OSTI]

    Appelfeld, David

    2013-01-01

    fenestration system (CFS). The challenge is to evaluatean interconnected context for CFS performance, since some ofdesign criteria for widows and CFS in modern buildings are:

  17. An hourly-based performance comparison of an integrated micro-structural perforated shading screen with standard shading systems

    E-Print Network [OSTI]

    Appelfeld, David

    2013-01-01

    complex fenestration systems, Solar Energy, submitted foron prismatic glazing, Solar Energy 74 (2003) 157–173. [13]of glazings and windows, Solar Energy 69 (Suppl. 1–6) (2000)

  18. Biomarkers of acute respiratory allergen exposure: Screening for sensitization potential

    SciTech Connect (OSTI)

    Pucheu-Haston, Cherie M.; Copeland, Lisa B.; Vallanat, Beena; Boykin, Elizabeth; Ward, Marsha D.W.

    2010-04-15

    Effective hazard screening will require the development of high-throughput or in vitro assays for the identification of potential sensitizers. The goal of this preliminary study was to identify potential biomarkers that differentiate the response to allergens vs non-allergens following an acute exposure in naive individuals. Female BALB/c mice received a single intratracheal aspiration exposure to Metarhizium anisopliae crude antigen (MACA) or bovine serum albumin (BSA) in Hank's Balanced Salt Solution (HBSS) or HBSS alone. Mice were terminated after 1, 3, 6, 12, 18 and 24 h. Bronchoalveolar lavage fluid (BALF) was evaluated to determine total and differential cellularity, total protein concentration and LDH activity. RNA was isolated from lung tissue for microarray analysis and qRT-PCR. MACA administration induced a rapid increase in BALF neutrophils, lymphocytes, eosinophils and total protein compared to BSA or HBSS. Microarray analysis demonstrated differential expression of genes involved in cytokine production, signaling, inflammatory cell recruitment, adhesion and activation in 3 and 12 h MACA-treated samples compared to BSA or HBSS. Further analyses allowed identification of approx 100 candidate biomarker genes. Eleven genes were selected for further assessment by qRT-PCR. Of these, 6 demonstrated persistently increased expression (Ccl17, Ccl22, Ccl7, Cxcl10, Cxcl2, Saa1), while C3ar1 increased from 6-24 h. In conclusion, a single respiratory exposure of mice to an allergenic mold extract induces an inflammatory response which is distinct in phenotype and gene transcription from the response to a control protein. Further validation of these biomarkers with additional allergens and irritants is needed. These biomarkers may facilitate improvements in screening methods.

  19. Screening study for evaluation of the potential for system 80+ to consume excess plutonium - Volume 1. Final report

    SciTech Connect (OSTI)

    Not Available

    1994-04-30

    As part of the U.S. effort to evaluate technologies offering solutions for the safe disposal or utilization of surplus nuclear materials, the fiscal year 1993 Energy and Water Appropriations legislation provided the Department of Energy (DOE) the necessary funds to conduct multi-phased studies to determine the technical feasibility of using reactor technologies for the triple mission of burning weapons grade plutonium, producing tritium for the existing smaller weapons stockpile, and generating commercial electricity. DOE limited the studies to five advanced reactor designs. Among the technologies selected is the ABB-Combustion Engineering (ABB-CE) System 80+. The DOE study, currently in Phase ID, is proceeding with a more detailed evaluation of the design`s capability for plutonium disposition.

  20. Screening study for evaluation of the potential for system 80+ to consume excess plutonium - Volume 2. Final report

    SciTech Connect (OSTI)

    Not Available

    1994-04-30

    As part of the U.S. effort to evaluate technologies offering solutions for the safe disposal or utilization of surplus nuclear materials, the fiscal year 1993 Energy and Water Appropriations legislation provided the Department of Energy (DOE) the necessary funds to conduct multi-phased studies to determine the technical feasibility of using reactor technologies for the triple mission of burning weapons grade plutonium, producing tritium for the existing smaller weapons stockpile, and generating commercial electricity. DOE limited the studies to five advanced reactor designs. Among the technologies selected is the ABB-Combustion Engineering (ABB-CE) System 80+. The DOE study, currently in Phase ID, is proceeding with a more detailed evaluation of the design`s capability for plutonium disposition.

  1. Inverse problems in systems biology This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Schuster, Peter

    ]. The experimental data base for systems biology comes from various high-throughput technologies applied in fields, consideration of the interplay between the parts of systems is indispensable for analyzing, modeling experimental techniques and computational methods in order to construct predictive models. Both in building

  2. Algorithm for Screening Phasor Measurement Unit Data for Power System Events and Categories and Common Characteristics for Events Seen in Phasor Measurement Unit Relative Phase-Angle Differences and Frequency Signals

    SciTech Connect (OSTI)

    Allen, A.; Santoso, S.; Muljadi, E.

    2013-08-01

    A network of multiple phasor measurement units (PMU) was created, set up, and maintained at the University of Texas at Austin to obtain actual power system measurements for power system analysis. Power system analysis in this report covers a variety of time ranges, such as short- term analysis for power system disturbances and their effects on power system behavior and long- term power system behavior using modal analysis. The first objective of this report is to screen the PMU data for events. The second objective of the report is to identify and describe common characteristics extracted from power system events as measured by PMUs. The numerical characteristics for each category and how these characteristics are used to create selection rules for the algorithm are also described. Trends in PMU data related to different levels and fluctuations in wind power output are also examined.

  3. Assessment of beating parameters in human induced pluripotent stem cells enables quantitative in vitro screening for cardiotoxicity

    SciTech Connect (OSTI)

    Sirenko, Oksana; Cromwell, Evan F.; Crittenden, Carole; Wignall, Jessica A.; Wright, Fred A.; Rusyn, Ivan

    2013-12-15

    Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes show promise for screening during early drug development. Here, we tested a hypothesis that in vitro assessment of multiple cardiomyocyte physiological parameters enables predictive and mechanistically-interpretable evaluation of cardiotoxicity in a high-throughput format. Human iPSC-derived cardiomyocytes were exposed for 30 min or 24 h to 131 drugs, positive (107) and negative (24) for in vivo cardiotoxicity, in up to 6 concentrations (3 nM to 30 uM) in 384-well plates. Fast kinetic imaging was used to monitor changes in cardiomyocyte function using intracellular Ca{sup 2+} flux readouts synchronous with beating, and cell viability. A number of physiological parameters of cardiomyocyte beating, such as beat rate, peak shape (amplitude, width, raise, decay, etc.) and regularity were collected using automated data analysis. Concentration–response profiles were evaluated using logistic modeling to derive a benchmark concentration (BMC) point-of-departure value, based on one standard deviation departure from the estimated baseline in vehicle (0.3% dimethyl sulfoxide)-treated cells. BMC values were used for cardiotoxicity classification and ranking of compounds. Beat rate and several peak shape parameters were found to be good predictors, while cell viability had poor classification accuracy. In addition, we applied the Toxicological Prioritization Index (ToxPi) approach to integrate and display data across many collected parameters, to derive “cardiosafety” ranking of tested compounds. Multi-parameter screening of beating profiles allows for cardiotoxicity risk assessment and identification of specific patterns defining mechanism-specific effects. These data and analysis methods may be used widely for compound screening and early safety evaluation in drug development. - Highlights: • Induced pluripotent stem cell-derived cardiomyocytes are promising in vitro models. • We tested if evaluation of cardiotoxicity is possible in a high-throughput format. • The assay shows benefits of automated data integration across multiple parameters. • Quantitative assessment of concentration–response is possible using iPSCs. • Multi-parametric screening allows for cardiotoxicity risk assessment.

  4. The Jaguar system consists of an 84 cabinet quad-core Cray XT4 system and 200 upgraded Cray XT5 cabinets, using six-core processors. The XT4 has 8 gigabytes of memory per node while the XT5 has 16

    E-Print Network [OSTI]

    "Budapest" processor coupled with 8 gigabytes of DDR2-800 memory. The XT5 is a double-density version Opteron has directly attached 8 gigabytes of DDR2-800 memory. The result is a dual-socket, twelve. High-throughput networks among its systems and upgraded connections to ESnet (Energy Sciences Network

  5. Alveolocapillary model system to study alveolar re-epithelialization

    SciTech Connect (OSTI)

    Willems, Coen H.M.P.; Zimmermann, Luc J.I.; Sanders, Patricia J.L.T.; Wagendorp, Margot; Kloosterboer, Nico; Cohen Tervaert, Jan Willem; Duimel, Hans J.Q.; Verheyen, Fons K.C.P.; Iwaarden, J. Freek van

    2013-01-01

    In the present study an in vitro bilayer model system of the pulmonary alveolocapillary barrier was established to investigate the role of the microvascular endothelium on re-epithelialization. The model system, confluent monolayer cultures on opposing sides of a porous membrane, consisted of a human microvascular endothelial cell line (HPMEC-ST1.6R) and an alveolar type II like cell line (A549), stably expressing EGFP and mCherry, respectively. These fluorescent proteins allowed the real time assessment of the integrity of the monolayers and the automated analysis of the wound healing process after a scratch injury. The HPMECs significantly attenuated the speed of re-epithelialization, which was associated with the proximity to the A549 layer. Examination of cross-sectional transmission electron micrographs of the model system revealed protrusions through the membrane pores and close contact between the A549 cells and the HPMECs. Immunohistochemical analysis showed that these close contacts consisted of heterocellular gap-, tight- and adherens-junctions. Additional analysis, using a fluorescent probe to assess gap-junctional communication, revealed that the HPMECs and A549 cells were able to exchange the fluorophore, which could be abrogated by disrupting the gap junctions using connexin mimetic peptides. These data suggest that the pulmonary microvascular endothelium may impact the re-epithelialization process. -- Highlights: ? Model system for vital imaging and high throughput screening. ? Microvascular endothelium influences re-epithelialization. ? A549 cells form protrusions through membrane to contact HPMEC. ? A549 cells and HPMECs form heterocellular tight-, gap- and adherens-junctions.

  6. Conventional Medical Screening Program

    Broader source: Energy.gov [DOE]

    Medical screening is a strategy used to identify diseases or conditions in a select population at an early stage, often before signs and symptoms develop, and to refer individuals with suspicious findings to their personal physician or a specialist for further testing, diagnosis, and treatment. The program is not intended to serve as a substitute for routine medical exams through an individual's personal physician.

  7. Instructions: Making the screen

    E-Print Network [OSTI]

    Mojzsis, Stephen J.

    of the Styrofoam cup as shown below: 2) Using a straight edge, draw a ling perpendicular to the slit as shown below of a Styrofoam cup using masking tape as in the figure below: Flashlight Tape #12 the zero centimeter line with the front of the screen and the edge of the meter stick against the Styrofoam

  8. EL INCIDENTE Film Screening

    E-Print Network [OSTI]

    EL INCIDENTE Film Screening A PART OF THE "SAWYER SEMINAR ON ALTERNATIVE FUTURISMS" Director Isaac Ezban's science fiction and horror films have been well received at international film festivals, with his first feature length film El Incidente winning awards for Best Original Screenplay for a Feature

  9. Cosmic Web and Environmental Dependence of Screening: Vainshtein vs. Chameleon

    E-Print Network [OSTI]

    Bridget Falck; Kazuya Koyama; Gong-bo Zhao

    2015-07-30

    Theories which modify general relativity to explain the accelerated expansion of the Universe often use screening mechanisms to satisfy constraints on Solar System scales. We investigate the effects of the cosmic web and the local environmental density of dark matter halos on the screening properties of the Vainshtein and chameleon screening mechanisms. We compare the cosmic web morphology of dark matter particles, mass functions of dark matter halos, mass and radial dependence of screening, velocity dispersions and peculiar velocities, and environmental dependence of screening mechanisms in $f(R)$ and nDGP models. Using the ORIGAMI cosmic web identification routine we find that the Vainshtein mechanism depends on the cosmic web morphology of dark matter particles, since these are defined according to the dimensionality of their collapse, while the chameleon mechanism shows no morphology dependence. The chameleon screening of halos and their velocity dispersions depend on halo mass, and small halos and subhalos can be environmentally screened in the chameleon mechanism. On the other hand, the screening of halos in the Vainshtein mechanism does not depend on mass nor environment, and their velocity dispersions are suppressed. The peculiar velocities of halos in the Vainshtein mechanism are enhanced because screened objects can still feel the fifth force generated by external fields, while peculiar velocities of chameleon halos are suppressed when the halo centers are screened.

  10. Cosmic Web and Environmental Dependence of Screening: Vainshtein vs. Chameleon

    E-Print Network [OSTI]

    Falck, Bridget; Zhao, Gong-bo

    2015-01-01

    Theories which modify general relativity to explain the accelerated expansion of the Universe often use screening mechanisms to satisfy constraints on Solar System scales. We investigate the effects of the cosmic web and the local environmental density of dark matter halos on the screening properties of the Vainshtein and chameleon screening mechanisms. We compare the cosmic web morphology of dark matter particles, mass functions of dark matter halos, mass and radial dependence of screening, velocity dispersions and peculiar velocities, and environmental dependence of screening mechanisms in $f(R)$ and nDGP models. Using the ORIGAMI cosmic web identification routine we find that the Vainshtein mechanism depends on the cosmic web morphology of dark matter particles, since these are defined according to the dimensionality of their collapse, while the chameleon mechanism shows no morphology dependence. The chameleon screening of halos and their velocity dispersions depend on halo mass, and small halos and subhal...

  11. Lock Your Computer Screen Before Leaving Your Desk

    E-Print Network [OSTI]

    California at Santa Barbara, University of

    Lock Your Computer Screen Before Leaving Your Desk Locking your computer's screen is an easy and effective step to take to protect the information on your computer system while it's unattended. Create Virus Protection Computers are infected with a virus more often than most people. Make sure antivirus

  12. Page 1 of 9 Full Screen

    E-Print Network [OSTI]

    Levene, Mark

    Evolution of Database Systems Graph-Based Relational Object-Relational 1960's-1970's 1980's-1990's 1990's-Oriented programming language with a DBMS 3 One approach is to implement on top of a relational DBMS. · Object #12;Home Page Title Page Page 5 of 9 Go Back Full Screen Close Quit Object-Relational Evolution

  13. www.emsl.pnnl.gov New Decade, New Tools, New Science: SyStemS biology

    E-Print Network [OSTI]

    , including high-throughput genomics, transcriptomics, proteomics, and metabolomics analyses as well as high

  14. Dynamic Screening and Thermonuclear Reaction Rates

    E-Print Network [OSTI]

    Andrei V. Gruzinov

    1997-02-06

    We show that there are no dynamic screening corrections to the Salpeter's enhancement factor in the weak-screening limit.

  15. Screening Analysis for the Environmental Risk Evaluation System Task 2.1.1.2: Evaluating Effects of Stressors Fiscal Year 2011 Progress Report - Environmental Effects of Marine and Hydrokinetic Energy

    SciTech Connect (OSTI)

    Copping, Andrea E.; Blake, Kara M.; Anderson, Richard M.; Zdanski, Laura C.; Gill, Gary A.; Ward, Jeffrey A.

    2011-09-01

    Potential environmental effects of marine and hydrokinetic (MHK) energy development are not well understood, and yet regulatory agencies are required to make decisions in spite of substantial uncertainty about environmental impacts and their long-term consequences. An understanding of risks associated with interactions between MHK installations and aquatic receptors, including animals, habitats, and ecosystems, can help define key uncertainties and focus regulatory actions and scientific studies on interactions of most concern. As a first step in developing the Pacific Northwest National Laboratory (PNNL) Environmental Risk Evaluation System (ERES), PNNL scientists conducted a preliminary risk screening analysis on three initial MHK cases. During FY 2011, two additional cases were added: a tidal project in the Gulf of Maine using Ocean Renewable Power Company TidGenTM turbines and a wave project planned for the coast of Oregon using Aquamarine Oyster surge devices. Through an iterative process, the screening analysis revealed that top-tier stressors in the two FY 2011 cases were the dynamic effects of the device (e.g., strike), accidents/disasters, and effects of the static physical presence of the device (e.g., habitat alteration). Receptor interactions with these stressors at the highest tiers of risk were dominated by threatened and endangered animals. Risk to the physical environment from changes in flow regime also ranked high. Peer review of this process and results will be conducted in early FY 2012. The ERES screening analysis provides an analysis of vulnerability of environmental receptors to stressors associated with MHK installations, probability analysis is needed to determine specific risk levels to receptors. “Risk” has two components: (1) The likelihood, or “probability”, of the occurrence of a given interaction or event, and (2) the potential “consequence” if that interaction or event were to occur. During FY 2011, the ERES screening analysis focused primarily on the second component of risk, “consequence”, with focused probability analysis for interactions where data was sufficient for probability modeling. Consequence analysis provides an assessment of vulnerability of environmental receptors to stressors associated with MHK installations. Probability analysis is needed to determine specific risk levels to receptors and requires significant data inputs to drive risk models. During FY 2011, two stressor-receptor interactions were examined for the probability of occurrence. The two interactions (spill probability due to an encounter between a surface vessel and an MHK device; and toxicity from anti-biofouling paints on MHK devices) were seen to present relatively low risks to marine and freshwater receptors of greatest concern in siting and permitting MHK devices. A third probability analysis was scoped and initial steps taken to understand the risk of encounter between marine animals and rotating turbine blades. This analysis will be completed in FY 2012.

  16. Updating Technical Screens for PV Interconnection: Preprint

    SciTech Connect (OSTI)

    Coddington, M.; Ellis, A.; Lynn, K.; Razon, A.; Key, T.; Kroposki, B.; Mather, B.; Hill, R.; Nicole, K.; Smith, J.

    2012-08-01

    Solar photovoltaics (PV) is the dominant type of distributed generation (DG) technology interconnected to electric distribution systems in the United States, and deployment of PV systems continues to increase rapidly. Considering the rapid growth and widespread deployment of PV systems in United States electric distribution grids, it is important that interconnection procedures be as streamlined as possible to avoid unnecessary interconnection studies, costs, and delays. Because many PV interconnection applications involve high penetration scenarios, the process needs to allow for a sufficiently rigorous technical evaluation to identify and address possible system impacts. Existing interconnection procedures are designed to balance the need for efficiency and technical rigor for all DG. However, there is an implicit expectation that those procedures will be updated over time in order to remain relevant with respect to evolving standards, technology, and practical experience. Modifications to interconnection screens and procedures must focus on maintaining or improving safety and reliability, as well as accurately allocating costs and improving expediency of the interconnection process. This paper evaluates the origins and usefulness of the capacity penetration screen, offers potential short-term solutions which could effectively allow fast-track interconnection to many PV system applications, and considers longer-term solutions for increasing PV deployment levels in a safe and reliable manner while reducing or eliminating the emphasis on the penetration screen.

  17. High-Throughput Bioscience Center (HTBC) History and Mission

    E-Print Network [OSTI]

    Kay, Mark A.

    pipettor (5-200 uL) Primary used for hit picking #12;SciClone ALH3000 Low Volume Head Can exchange heads3000 SciClone ALH3000, featuring... High Volume Head 96 disposal-tip pipettor (5-200 uL) Can change for 96 to 96 plate replication and 96 to 384 plate reformatting Z8 Head 8 independent disposable tip

  18. Algorithms for Next-Generation High-Throughput Sequencing Technologies

    E-Print Network [OSTI]

    Kao, Wei-Chun

    2011-01-01

    in the Human Genome Project(HGP) 1 . However, because Sangerprohibitive. In the case of HGP, it took more than 13 years

  19. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    x rays from one of the superconducting bend magnets at the ALS to liquid-handling robotics has enabled the collection of 96 samples in 4 hours. Importantly, the sample format...

  20. Microchip Technology for High-Throughput Cancer Pathology

    E-Print Network [OSTI]

    Duberow, Jr, David Paul

    2009-01-01

    Stranded DNA Analysis. Analytical Chemistry, 2007. 79(22):Electrophoresis Chips. Analytical Chemistry, 1995. 67(20):Acid Analysis. Analytical Chemistry, 1999. 71(23): p. 5354-

  1. Integrated microbioreactor arrays for high-throughput experimentation

    E-Print Network [OSTI]

    Lee, Harry, 1976-

    2006-01-01

    Bioprocesses with microbial cells play an important role in producing biopharmaceuticals such as human insulin and human growth hormone, and other products such as amino acids and biopolymers. Because bioprocesses involve ...

  2. High-throughput pipetting with the programmable Pipetman Concept

    E-Print Network [OSTI]

    Cai, Long

    Concept® takes advantage of a PC-to-pipette connection to outperform traditional motorized pipettes include aspiration, dispensing, dilution, mixing, repetitive dispensing and many more. A few minutes

  3. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    and large-area graphene plane as a floor. Preparation ofgraphene was dried and prepared for dye coating. Dye Preparation and

  4. Differential analysis of high-throughput quantitative genetic interaction data

    E-Print Network [OSTI]

    Bean, Gordon J; Ideker, Trey

    2012-01-01

    DNA damage response (DDR)). Other biological networks, suchespecially in context of the DDR. Thus, differentialThese gene pairs indicate DDR- relevant interactions that

  5. High throughput analysis of samples in flowing liquid

    DOE Patents [OSTI]

    Ambrose, W. Patrick (Los Alamos, NM); Grace, W. Kevin (Los Alamos, NM); Goodwin, Peter M. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Orden, Alan Van (Fort Collins, CO); Keller, Richard A. (White Rock, NM)

    2001-01-01

    Apparatus and method enable imaging multiple fluorescent sample particles in a single flow channel. A flow channel defines a flow direction for samples in a flow stream and has a viewing plane perpendicular to the flow direction. A laser beam is formed as a ribbon having a width effective to cover the viewing plane. Imaging optics are arranged to view the viewing plane to form an image of the fluorescent sample particles in the flow stream, and a camera records the image formed by the imaging optics.

  6. BLINK: A High Throughput Link Layer for Backscatter Communication

    E-Print Network [OSTI]

    Ganesan, Deepak

    in areas where small form-factor is critical (e.g. on-body sen- sors for gesture and activity recognition to active radios in urban sensing deployments -- com- munication is powered by a reader, thereby making sev- eral optimizations including 1) understanding of multi-path self-interference characteristics

  7. High-Throughput Transfer Imprinting for Organic Semiconductors 

    E-Print Network [OSTI]

    Choo, Gihoon

    2013-08-06

    .......................................................... 9 Figure 5 Major steps of UV nanoimprint process ................................................... 11 Figure 6 Two methods of roller nanoimprint lithography ....................................... 13 Figure 7 Steps of LADI process... of the photolithography process are illustrated in Figure1. The first step is coating UV sensitive photoresist on the substrate. A spin-coating method is widely used for the step. After coating, a short bake at about 90C is applied to remove solution from the resist...

  8. Statistical Algorithms for High-throughput Biological Data /

    E-Print Network [OSTI]

    Jeong, Kyowon

    2013-01-01

    peptide-level factual FDR and PSM-level FDR 100 Table 4.11:affects the results . . . 4.4.8 PSM-level vs Peptide-level2 A peptide-spectrum match (PSM) is a pair (P, S) formed by

  9. Microchip Technology for High-Throughput Cancer Pathology

    E-Print Network [OSTI]

    Duberow, Jr, David Paul

    2009-01-01

    S. , and Marra, M. Resuspension of DNA Sequencing Reactionstability. (A) Sample resuspension in low-concentrationand used as the sole resuspension buffer following sample

  10. High-throughput comparison of gene fitness among related bacteria

    E-Print Network [OSTI]

    2012-01-01

    5096–5108. 36. Macnab RM: How bacteria assemble. Annu Revgene fitness among related bacteria. BMC Genomics 2012 13:gene fitness among related bacteria Rocio Canals 1 , Xiao-

  11. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    adjusted as an in-line graphene measurement technique.fabrication and metrology of graphene materials. Our mainof Large-Area Hybrid Graphene-CNT Architectures 2.1

  12. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally...

  13. Low inlet gas velocity high throughput biomass gasifier

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.

    1989-05-09

    A method is described for operating a gasifier which comprises: introducing inlet gas at a velocity of about 0.5 to 7 ft/sec to fluidize a bed in a gasifier vessel; forming the bed into a fluidized bed in a first space region by means of the inlet gas, the fluidized bed containing a circulating hot relatively fine and inert solid bed particle component; inputting and throughputting carbonaceous material into and through the first space region with fluidized bed at a rate from 500-4400 lbs/ft/sup 2/-hr; endothermally pyrolyzing the carbonaceous material by means of the circulating hot inert particle component so as to form a product gas; forming contiguous to and above the fluidized bed a lower average density entrained space region containing an entrained mixture of inert solid particles, char, and carbonaceous material and the product gas; gradually and continuously removing the entrained mixture and the product gas from the lower average density entrained space region of the gasifier to a separator, residence time of the carbonaceous material in the gasifier not exceeding 3 minutes on average; separating the entrained mixture from the product gas; passing the entrained mixture containing inert solid particles, char, and carbonaceous material through an exothermic reaction zone to add heat; and returning at least the inert solid particles to the first space region.

  14. Low inlet gas velocity high throughput biomass gasifier

    SciTech Connect (OSTI)

    Feldmann, Herman F.; Paisley, Mark A.

    1989-01-01

    The present invention discloses a novel method of operating a gasifier for production of fuel gas from carbonaceous fuels. The process disclosed enables operating in an entrained mode using inlet gas velocities of less than 7 feet per second, feedstock throughputs exceeding 4000 lbs/ft.sup.2 -hr, and pressures below 100 psia.

  15. Algorithms for Next-Generation High-Throughput Sequencing Technologies

    E-Print Network [OSTI]

    Kao, Wei-Chun

    2011-01-01

    Algorithm . . . . . . . . . . . . . . . . . . . . . .2.6.1 A hybrid base-calling algorithm . . . . . . . . .Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . .

  16. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    described here, which the research team believes will help usher in the next phase of genomics research. The high flux, brilliance, and focus of the x rays from ALS Beamline 12.3.1...

  17. Optical Multiplexing for High-Throughput Spectroscopic Analysis. 

    E-Print Network [OSTI]

    Ahmed, Saadiah

    2012-05-03

    in optical biosensor testing as it provides greater throughput and scalability while being an economical and compact solution. v NOMENCLATURE MFPF Multi Frequency Phase Fluorometer MUX Multiplexer LED Light Emitting Diode DIO Digital Input... oxidase in hybrid silicate microsphere that is coated with nanofilm and reference dye (RITC) (Fig. 1) [2]. The phosphorescent dye is excited by a green light from a LED (light emitting diode) to emit luminescence, the intensity of which is dependent...

  18. Towards Experimental Annotation of Genes by High Throughput Sequencing

    SciTech Connect (OSTI)

    Bradbury, Andrew

    2010-06-03

    Andrew Bradbury of Los Alamos National Laboratory discusses turning annotation into a sequencing pipeline on June 3, 2010 at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM

  19. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    exfoliated graphene oxide layers along with CVD grown CNTsto create a barrier oxide layer on copper foil. Fe catalystcopper oxide foil, the oxide layer would be gradually flow,

  20. Computational tools for high-throughput discovery in biology

    E-Print Network [OSTI]

    Jones, Neil Christopher

    2007-01-01

    implementing “better” Grid security within a VO occurs whenPearlman, and S. Tuecke. Security for grid services. In 12thGrid), which is a sort of metaservice collection that handles indexing, discovery, security,

  1. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    graphene industrially, in areas such as energy, semiconductorgraphene to n- and p-type, which are required for complementary metal oxide semiconductor (

  2. Computational tools for high-throughput discovery in biology

    E-Print Network [OSTI]

    Jones, Neil Christopher

    2007-01-01

    Towards creating a compute power grid. Cluster Computing andfor all data processing: the power grid. With a well-frameddevelopers. Since this Power Grid analogy has more or less

  3. High Throughput Analysis of Breast Cancer Specimens on the Grid

    E-Print Network [OSTI]

    cancer accounts for about 30% of all cancers and 15% of all cancer deaths in women in the United States in the United States. Women living in North America have the highest rate of breast cancer in the world [1]. In spite of the increase in the incidence of the disease, the death rates of breast cancer continue

  4. Advanced method for high-throughput expression of mutated eukaryotic...

    Office of Scientific and Technical Information (OSTI)

    ; Murata, Takeshi 2 ; Department of Medical Chemistry, Kyoto University, Faculty of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501 4 ; Abe, Keiko 1 ; Iwata, So 2 ;...

  5. High-Throughput Single-Cell Manipulation in Brain Tissue

    E-Print Network [OSTI]

    Steinmeyer, Joseph Daly

    The complexity of neurons and neuronal circuits in brain tissue requires the genetic manipulation, labeling, and tracking of single cells. However, current methods for manipulating cells in brain tissue are limited to ...

  6. Microchip Technology for High-Throughput Cancer Pathology

    E-Print Network [OSTI]

    Duberow, Jr, David Paul

    2009-01-01

    30 V/cm), followed by direct injection of analyte from thethen tested, although direct injection of 4 µL (12 x 10 6is observed following direct injection from waste to anode (

  7. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    the automation of the technique for production-linethe automation of the technique for production-line

  8. Potential of High-Throughput Experimentation with Ammonia Borane Solid

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankADVANCED MANUFACTURINGEnergy Bills andOrder 422.1,anEnergyDepartment ofPotential Health-Hydrogen

  9. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration

  10. High-Throughput Analytical Model to Evaluate Materials for Temperature

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid you notHeat Pumps Heat Pumps An error occurred. Try|High-Powered

  11. High-Throughput Characterization of Porous Materials Using Graphics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid you notHeat Pumps Heat Pumps An error occurred.

  12. Advanced method for high-throughput expression of mutated eukaryotic

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnicalInformation4563Abuse Tolerance(Conference) |stabilizedmembrane proteins in

  13. High-throughput metagenomic technologies for complex microbial community

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article)lasers (Journal Article)SciTechHigh-contrastproperties ofwallanalysis.

  14. High-throughput metagenomic technologies for complex microbial community

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article)lasers (Journal Article)SciTechHigh-contrastproperties

  15. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation of Fe(II) byMultidayAlumni > The Energy Materials CenterRobotics Key to

  16. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation of Fe(II) byMultidayAlumni > The Energy Materials CenterRobotics Key toRobust,

  17. Sandia National Laboratories: Quantum Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-ThroughputUpcoming Release of thePrograms:Mode Stirred ChamberReuseQuantum Systems

  18. Mixed Alcohol Synthesis Catalyst Screening

    SciTech Connect (OSTI)

    Gerber, Mark A.; White, James F.; Stevens, Don J.

    2007-09-03

    National Renewable Energy Laboratory (NREL) and Pacific Northwest National Laboratory (PNNL) are conducting research to investigate the feasibility of producing mixed alcohols from biomass-derived synthesis gas (syngas). PNNL is tasked with obtaining commercially available or preparing promising mixed-alcohol catalysts and screening them in a laboratory-scale reactor system. Commercially available catalysts and the most promising experimental catalysts are provided to NREL for testing using a slipstream from a pilot-scale biomass gasifier. From the standpoint of producing C2+ alcohols as the major product, it appears that the rhodium catalyst is the best choice in terms of both selectivity and space-time yield (STY). However, unless the rhodium catalyst can be improved to provide minimally acceptable STYs for commercial operation, mixed alcohol synthesis will involve significant production of other liquid coproducts. The modified Fischer-Tropsch catalyst shows the most promise for providing both an acceptable selectivity to C2+ alcohols and total liquid STY. However, further optimization of the Fischer-Tropsch catalysts to improve selectivity to higher alcohols is highly desired. Selection of a preferred catalyst will likely entail a decision on the preferred coproduct slate. No other catalysts tested appear amenable to the significant improvements needed for acceptable STYs.

  19. Objective: Determine the energy use of two greenhouse insulation technologies (a bubble insulation system and an energy/shade screen) retrofitted into plastic covered greenhouses, and compare the

    E-Print Network [OSTI]

    Vermont, University of

    Objective: Determine the energy use of two greenhouse insulation technologies (a bubble insulation system and energy curtain provided significant reductions in natural gas use and associated heating costs Resources and Conservation Service, New Hampshire Floriculture Endowment and SunArc, Canada. 2013 Energy Use

  20. Rare earth phosphors and phosphor screens

    DOE Patents [OSTI]

    Buchanan, Robert A. (Palo Alto, CA); Maple, T. Grant (Sunnyvale, CA); Sklensky, Alden F. (Sunnyvale, CA)

    1981-01-01

    This invention relates to rare earth phosphor screens for converting image carrying incident radiation to image carrying visible or near-visible radiation and to the rare earth phosphor materials utilized in such screens. The invention further relates to methods for converting image carrying charged particles to image carrying radiation principally in the blue and near-ultraviolet region of the spectrum and to stabilized rare earth phosphors characterized by having a continuous surface layer of the phosphors of the invention. More particularly, the phosphors of the invention are oxychlorides and oxybromides of yttrium, lanthanum and gadolinium activated with trivalent cerium and the conversion screens are of the type illustratively including x-ray conversion screens, image amplifier tube screens, neutron imaging screens, cathode ray tube screens, high energy gamma ray screens, scintillation detector screens and screens for real-time translation of image carrying high energy radiation to image carrying visible or near-visible radiation.

  1. Screening length in plasma winds

    E-Print Network [OSTI]

    Elena Caceres; Makoto Natsuume; Takashi Okamura

    2007-06-04

    We study the screening length L_s of a heavy quark-antiquark pair in strongly coupled gauge theory plasmas flowing at velocity v. Using the AdS/CFT correspondence we investigate, analytically, the screening length in the ultra-relativistic limit. We develop a procedure that allows us to find the scaling exponent for a large class of backgrounds. We find that for conformal theories the screening length is (boosted energy density)^{-1/d}. As examples of conformal backgrounds we study R-charged black holes and Schwarzschild-anti-deSitter black holes in (d+1)-dimensions. For non-conformal theories, we find that the exponent deviates from -1/d and as examples we study the non-extremal Klebanov-Tseytlin and Dp-brane geometries. We find an interesting relation between the deviation of the scaling exponent from the conformal value and the speed of sound.

  2. Control System Design for Automatic Cavity Tuning Machines

    SciTech Connect (OSTI)

    Carcagno, R.; Khabiboulline, T.; Kotelnikov, S.; Makulski, A.; Nehring, R.; Nogiec, J.; Ross, M.; Schappert, W.; /Fermilab; Goessel, A.; Iversen, J.; Klinke, D.; /DESY

    2009-05-01

    A series of four automatic tuning machines for 9-cell TESLA-type cavities are being developed and fabricated in a collaborative effort among DESY, FNAL, and KEK. These machines are intended to support high-throughput cavity fabrication for construction of large SRF-based accelerator projects. Two of these machines will be delivered to cavity vendors for the tuning of XFEL cavities. The control system for these machines must support a high level of automation adequate for industrial use by non-experts operators. This paper describes the control system hardware and software design for these machines.

  3. Updating Interconnection Screens for PV System Integration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust,Field-effectWorking With WIPPfinalUnexpectedofWyko NT3300 OpticalUpdate onMayMichael

  4. DOE: Former Worker Medical Screening Program (FWP)

    Broader source: Energy.gov [DOE]

    FWP provides no-cost medical screenings to all former DOE Federal, contractor and subcontractor employees. The screening exams are offered by third party providers from universities, labor unions,...

  5. Portuguese Ships on Japanese Namban Screens 

    E-Print Network [OSTI]

    Yamafune, Kotaro

    2012-10-19

    scenes of the first European activities in Japan. Among the subjects depicted on Namban screens, some of the most intriguing are ships: the European ships of the Age of Discovery. Namban screens were created by skillful Japanese traditional painters who...

  6. Generalized charge-screening in relativistic Thomas–Fermi model

    SciTech Connect (OSTI)

    Akbari-Moghanjoughi, M.

    2014-10-15

    In this paper, we study the charge shielding within the relativistic Thomas-Fermi model for a wide range of electron number-densities and the atomic-number of screened ions. A generalized energy-density relation is obtained using the force-balance equation and taking into account the Chandrasekhar's relativistic electron degeneracy pressure. By numerically solving a second-order nonlinear differential equation, the Thomas-Fermi screening length is investigated, and the results are compared for three distinct regimes of the solid-density, warm-dense-matter, and white-dwarfs (WDs). It is revealed that our nonlinear screening theory is compatible with the exponentially decaying Thomas-Fermi-type shielding predicted by the linear response theory. Moreover, the variation of relative Thomas-Fermi screening length shows that extremely dense quantum electron fluids are relatively poor charge shielders. Calculation of the total number of screening electrons around a nucleus shows that there is a position of maximum number of screening localized electrons around the screened nucleus, which moves closer to the point-like nucleus by increase in the plasma number density but is unaffected due to increase in the atomic-number value. It is discovered that the total number of screening electrons, (N{sub s}?r{sub TF}{sup 3}/r{sub d}{sup 3} where r{sub TF} and r{sub d} are the Thomas-Fermi and interparticle distance, respectively) has a distinct limit for extremely dense plasmas such as WD-cores and neutron star crusts, which is unique for all given values of the atomic-number. This is equal to saying that in an ultrarelativistic degeneracy limit of electron-ion plasma, the screening length couples with the system dimensionality and the plasma becomes spherically self-similar. Current analysis can provide useful information on the effects of relativistic correction to the charge screening for a wide range of plasma density, such as the inertial-confined plasmas and compact stellar objects.

  7. Screening genomes of Gram-positive bacteria for

    E-Print Network [OSTI]

    Screening genomes of Gram-positive bacteria for double-glycine-motif- containing peptides Secreted-positive bacteria, the double-glycine (GG) motif plays a key role in many peptide secretion systems involved Microbiology Comment #12;peptides and class II bacteriocins, produced by streptococci and lactic acid bacteria

  8. Long-Term Planning for Nuclear Energy Systems Under Deep Uncertainty

    E-Print Network [OSTI]

    Kim, Lance Kyungwoo

    2011-01-01

    and E. Davies. Uranium Enrichment by Gas Centrifuge. Mills ¢rifuges, capable of producing a high throughput of low enriched uranium (

  9. Coal storage hopper with vibrating screen agitator

    DOE Patents [OSTI]

    Daw, Charles S. (Knoxville, TN); Lackey, Mack E. (Oak Ridge, TN); Sy, Ronald L. (Clinton, TN)

    1984-01-01

    The present invention is directed to a vibrating screen agitator in a coal storage hopper for assuring the uniform feed of coal having sufficient moisture content to effect agglomeration and bridging thereof in the coal hopper from the latter onto a conveyor mechanism. The vibrating screen agitator is provided by a plurality of transversely oriented and vertically spaced apart screens in the storage hopper with a plurality of vertically oriented rods attached to the screens. The rods are vibrated to effect the vibration of the screens and the breaking up of agglomerates in the coal which might impede the uniform flow of the coal from the hopper onto a conveyer.

  10. This new system will allow researchers to much more rapidly screen large numbers of samples and identify the most promising biomass feedstocks for higher efficiency and lower cost bio-

    E-Print Network [OSTI]

    of samples and identify the most promising biomass feedstocks for higher efficiency and lower cost bio- fuels conversion processes. NREL will be screening thousands of variants of different biomass feedstocks to link to develop the next generation of low-cost, easily convert- ible biomass feedstocks. To identify superior

  11. TECHNOLOGY REPORT Development of a Gene-Trap Vector With a Highly

    E-Print Network [OSTI]

    Zandstra, Peter W.

    TECHNOLOGY REPORT Development of a Gene-Trap Vector With a Highly Sensitive Fluorescent Protein 23 March 2008; Revised 27 April 2008; Accepted 6 May 2008 Summary: Combining high-content screening and Twist2. This highly sensitive reporter system is amenable to high-throughput expression- based real

  12. Development of a Green Fluorescent Protein-Based Cell Bioassay for the Rapid and Inexpensive Detection and Characterization

    E-Print Network [OSTI]

    Hammock, Bruce D.

    inducing potency of a series of previously uncharac- terized dioxin surrogates. This bioassay system has of use, low reagent cost, and application for high-throughput screening. Key Words: dioxin, Ah receptor (dioxin-responsive elements or DREs) (Deni- son et al., 1998a; Whitlock, 1999). Although ligands for Ah

  13. Precise manipulation of GUI on a touch screen with haptic cues

    E-Print Network [OSTI]

    Srinivasan, Mandayam A.

    In this work, we designed a haptic stylus interface interacting with a touch screen. The stylus has functions of providing vibration, impact, and sound and it is a stand-alone system including its own battery and wireless ...

  14. Reverberatory screen for a radiant burner

    DOE Patents [OSTI]

    Gray, Paul E. (North East, MD)

    1999-01-01

    The present invention relates to porous mat gas fired radiant burner panels utilizing improved reverberatory screens. The purpose of these screens is to boost the overall radiant output of the burner relative to a burner using no screen and the same fuel-air flow rates. In one embodiment, the reverberatory screen is fabricated from ceramic composite material, which can withstand higher operating temperatures than its metallic equivalent. In another embodiment the reverberatory screen is corrugated. The corrugations add stiffness which helps to resist creep and thermally induced distortions due to temperature or thermal expansion coefficient differences. As an added benefit, it has been unexpectedly discovered that the corrugations further increase the radiant efficiency of the burner. In a preferred embodiment, the reverberatory screen is both corrugated and made from ceramic composite material.

  15. Coiled tubing cuts horizontal screen repair cost

    SciTech Connect (OSTI)

    Crow, W.; Hill, P.; Johnston, R.

    1996-01-01

    This article presents a case history of the successful workover performed by a coiled tubing unit (CTU) on Mississippi Canyon (MC) Block 109 Well A-24 in the US Gulf of Mexico to clean out sand and install new concentric screen for sand control. Workover design and operational details discussed are: Workover design -- hole-cleaning hydraulics, CT and screen predictions and comparison considerations; Workover operations -- cleanout, running packer and screens, coiled tubing (CT) weights, acid treatment and nitrogen lift and flow back.

  16. Vermont Small Hydropower Assistance Program Screening Criteria...

    Open Energy Info (EERE)

    Vermont Small Hydropower Assistance Program Screening Criteria Summary and Application Instructions Jump to: navigation, search OpenEI Reference LibraryAdd to library Permitting...

  17. Screen Electrode Materials & Cell Chemistries and Streamlining...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    & Cell Chemistries and Streamlining Optimization of Electrode Screen Electrode Materials & Cell Chemistries and Streamlining Optimization of Electrode 2010 DOE Vehicle Technologies...

  18. CHEST Supplement Screening for Lung Cancer

    E-Print Network [OSTI]

    O'Hern, Corey S.

    e78S CHEST Supplement Screening for Lung Cancer DIAGNOSIS AND MANAGEMENT OF LUNG CANCER, 3RD ED individuals). 3.2.2. In patients at risk for developing lung cancer, screening for lung cancer with sputum are age 55 to 74 and who have smoked for Background: Lung cancer is by far the major cause of cancer

  19. www.yalecancercenter.org Lung Cancer Screening

    E-Print Network [OSTI]

    O'Hern, Corey S.

    www.yalecancercenter.org Lung Cancer Screening Guest Experts: Lynn Tanoue, MD Professor of Therapeutic Radiology and an expert in the use of radiation to treat lung cancers and cutaneous lymphomas Oncology Program. Dr. Detterbeck and I also co- direct the new Yale Lung Cancer and Lung Nodule Screening

  20. Fusion Protein Products Screen Purify Detect Cleave

    E-Print Network [OSTI]

    Lebendiker, Mario

    Fusion Protein Products · Screen · Purify · Detect · Cleave Fusion Protein Products · Screen researchers look to plasmid vectors to express fusion proteins, they find themselves in need of methods proteins is also included for those fusion proteins that may have an inaccessible tag. Pierce offers a host

  1. Automated imaging system for single molecules

    DOE Patents [OSTI]

    Schwartz, David Charles; Runnheim, Rodney; Forrest, Daniel

    2012-09-18

    There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure allow automated collection and initial processing of optical images of single molecules and their assemblies. Correct focus may be automatically maintained while images are collected. Uneven illumination in fluorescence microscopy is accounted for, and an overall robust imaging operation is provided yielding individual images prepared for further processing in external systems. Embodiments described herein are useful in studies of any macromolecules such as DNA, RNA, peptides and proteins. The automated image collection and processing system and method of same may be implemented and deployed over a computer network, and may be ergonomically optimized to facilitate user interaction.

  2. How to estimate solid-electrolyte interphase features when screening electrolyte materials

    E-Print Network [OSTI]

    Husch, Tamara

    2015-01-01

    Computational screening of battery electrolyte components is an extremely challenging task because very complex features like solidelectrolyte-interphase (SEI) formation and graphite exfoliation need to be taken into account at least at the final screening stage. We present estimators for both SEI formation and graphite exfoliation based on a combinatorial approach with quantum chemistry calculations on model system reactions, which can be applied automatically for a large number of compounds and thus allows for a systematic first assessment of the relevant properties within screening approaches. Thermodynamic effects are assessed with quantum mechanical calculations, a more heuristic approach is used to estimate kinetic effects.

  3. Sandia Energy - Wake-Imaging System Progresses to Outdoor Scaled Test

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput AnalysisSinkholeCapabilities GeneralWake-Imaging System Progresses to

  4. Observation of finite-wavelength screening in high-energy-density matter

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chapman, D. A.; Vorberger, J.; Fletcher, L. B.; Baggott, R. A.; Divol, L.; Döppner, T.; Falcone, R. W.; Glenzer, S. H.; Gregori, G.; Guymer, T. M.; et al

    2015-04-23

    A key component for the description of charged particle systems is the screening of the Coulomb interaction between charge carriers. First investigated in the 1920s by Debye and Hückel for electrolytes, charge screening is important for determining the structural and transport properties of matter as diverse as astrophysical and laboratory plasmas, nuclear matter such as quark-gluon plasmas, electrons in solids, planetary cores and charged macromolecules. For systems with negligible dynamics, screening is still mostly described using a Debye–Hückel-type approach. Here, we report the novel observation of a significant departure from the Debye–Hückel-type model in high-energy-density matter by probing laser-driven, shock-compressedmore »plastic with high-energy X-rays. We use spectrally resolved X-ray scattering in a geometry that enables direct investigation of the screening cloud, and demonstrate that the observed elastic scattering amplitude is only well described within a more general approach.« less

  5. Schrödinger Spacetimes with Screen and Reduced Entanglement

    E-Print Network [OSTI]

    Harvendra Singh

    2014-10-15

    We study a particular class of type II string vacua which become Schr\\"odinger like spacetime in the IR region but are conformally AdS in asymptotic UV region. These solutions are found to possess some unique properties such as the presence of a spacetime `screen'. This Schr\\"odinger (spacetime) screen is however very different from a black-hole horizon. It requires the presence of finite chemical potential and a negative charge density in the Schr\\"odinger CFT. We find that these vacua give rise to reduced entanglement entropy as compared to Lifshitz-AdS counterpart, perhaps due to the screening effects.

  6. FEMP Completes 2000th Renewable Energy Optimization Screening...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Completes 2000th Renewable Energy Optimization Screening FEMP Completes 2000th Renewable Energy Optimization Screening July 23, 2015 - 12:03pm Addthis REopt models the complex...

  7. HT Combinatorial Screening of Novel Materials for High Capacity...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen Storage HT Combinatorial Screening of Novel Materials for High Capacity Hydrogen Storage Presentation for...

  8. ANALYSIS OF DUST DELIQUESCENCE FOR FEP SCREENING

    SciTech Connect (OSTI)

    C. Bryan

    2005-08-26

    The purpose of this report is to evaluate the potential for penetration of the Alloy 22 (UNS N06022) waste package outer barrier by localized corrosion due to the deliquescence of soluble constituents in dust present on waste package surfaces. The results support a recommendation to exclude deliquescence-induced localized corrosion (pitting or crevice corrosion) of the outer barrier from the total system performance assessment for the license application (TSPA-LA). Preparation of this report, and supporting laboratory studies and calculations, were performed as part of the planned effort in Work Package AEBM21, as implemented in ''Technical Work Plan for: Screening Evaluation for Dust Deliquescence and Localized Corrosion'' (BSC 2004 [DIRS 172804]), by Bechtel SAIC Company, LLC, and staff from three national laboratories: Sandia National Laboratories, Lawrence Livermore National Laboratory (LLNL), and Lawrence Berkeley National Laboratory (LBNL). The analysis and conclusions presented in this report are quality affecting, as determined in the controlling technical work plan. A summary of background information, based on work that was not performed under a quality assurance program, is provided as Appendix E. In this instance, the use of unqualified information is provided for transparency and corroboration only, and is clearly separated from uses of qualified information. Thus, the qualification status of this information does not affect the conclusions of this report. The acceptance criteria addressed in Sections 4.2 and 7.2 were changed from the technical work plan in response to review comments received during preparation of this report.

  9. Advanced Millimeter-Wave Imaging Enhances Security Screening

    SciTech Connect (OSTI)

    Sheen, David M.; Bernacki, Bruce E.; McMakin, Douglas L.

    2012-01-12

    Millimeter-wave imaging is rapidly gaining acceptance for passenger screening at airports and other secured facilities. This paper details a number of techniques developed over the last several years including novel image reconstruction and display techniques, polarimetric imaging techniques, array switching schemes, as well as high frequency high bandwidth techniques. Implementation of some of these methods will increase the cost and complexity of the mm-wave security portal imaging systems. RF photonic methods may provide new solutions to the design and development of the sequentially switched linear mm-wave arrays that are the key element in the mm-wave portal imaging systems.

  10. Visceral material: cinematic bodies on screen 

    E-Print Network [OSTI]

    Bugaj, Malgorzata

    2014-11-25

    This thesis investigates cinema’s attempts to engage in a dialogue with the trace of the physical body. My concern is with the on-screen presentation of the body rather than its treatment as a representation of gender, ...

  11. March 2013 LabNotes | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    etc. ) to slowly convert stored CO2 into more useful products such as methane and methanol. NETL Studies High Throughput Membrane Screening Membranes offer a potential...

  12. Power production from renewable resources in a gasification power system

    SciTech Connect (OSTI)

    Paisley, M.A.; Farris, G.; Bain, R.

    1996-12-31

    The US Department of Energy (DOE) has been a leader in the promotion and development of alternative fuel supplies based on renewable energy crops. One promising power generation technology is biomass gasification coupled with either a gas turbine in a combined cycle system or a fuel cell. The gasification of biomass can efficiently and economically produce a renewable source of a clean gaseous fuel suitable for use in these high efficiency power systems or as a substitute fuel in other combustion devices such as boilers, kilns, or other natural gas fired equipment. This paper discusses the development and commercialization of the Battelle high-throughput gasification process for gas turbine based power generation systems. Projected process economics for a gas turbine combined cycle plant are presented along with a description of integrated system operation coupling a 200kW gas turbine power generation system to a 10 ton per day gasifier, and current commercialization activities. 6 refs., 3 figs., 1 tab.

  13. Supplementary Files: Screening negative compound-protein samples

    E-Print Network [OSTI]

    Brendel, Volker

    compounds/proteins. Table S1. AUC/Recall/Precision values of six classical classifiers on screened and randomly generated negative samples of C. elegans (pairwise cross-validation). Measure Negative sample ratio Naive Bayes kNN Random Forest L1-logistic L2-logistic SVM screened random screened random screened

  14. 111Screening Young Children for Lead Poisoning Chapter 5: Resources

    E-Print Network [OSTI]

    . In the future, CDC will support grantees in developing and disseminating screening recommendations. #12

  15. Development of an Automated Microfluidic Reaction Platform for Multidimensional Screening: Reaction Discovery Employing Bicyclo[3.2.1]octanoid Scaffolds

    E-Print Network [OSTI]

    Goodell, John R.

    An automated, silicon-based microreactor system has been developed for rapid, low-volume, multidimensional reaction screening. Use of the microfluidic platform to identify transformations of densely functionalized bicyclo ...

  16. Screening Tool for Providers of Double-Stranded DNA - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-ThroughputUpcomingmagnetoresistance | ArgonnePrinceton Plasma

  17. The Shape Dependence of Vainshtein Screening

    E-Print Network [OSTI]

    Jolyon K. Bloomfield; Clare Burrage; Anne-Christine Davis

    2015-08-17

    Scalar field theories that possess a Vainshtein mechanism are able to dynamically suppress the associated fifth forces in the presence of massive sources through derivative non-linearities. The resulting equations of motion for the scalar are highly non-linear and therefore very few analytic solutions are known. Here we present a brief investigation of the structure of Vainshtein screening in symmetrical configurations, focusing in particular on the spherical, cylindrical and planar solutions that are relevant for observations of the cosmic web. We consider Vainshtein screening in both the Galileon model, where the non-linear terms involve second derivatives of the scalar, and a k-essence theory, where the non-linear terms involve only first derivatives of the scalar. We find that screening, and consequently the suppression of the scalar force, is most efficient around spherical sources, weaker around cylindrical sources and can be absent altogether around planar sources.

  18. Confinement and screening in tachyonic matter

    E-Print Network [OSTI]

    F. A. Brito; M. L. F. Freire; W. Serafim

    2014-11-20

    In this paper we consider confinement and screening of the electric field. We study the behavior of a static electric field coupled to a dielectric function with the intent of obtaining an electrical confinement similar to what happens with the field of gluons that bind quarks in hadronic matter. For this we use the phenomenon of `anti-screening' in a medium with exotic dielectric. We show that tachyon matter behaves like an exotic way whose associated dielectric function modifies the Maxwell's equations and affects the fields which results in confining and Coulombian-like potentials in three spatial dimensions. We note that the confining regime coincides with the tachyon condensation, which resembles the effect of confinement due to condensation of magnetic monopoles. The Coulombian-like regime is developed at large distance which is associated with {a screening phase

  19. Water Velocity Measurements on a Vertical Barrier Screen at the Bonneville Dam Second Powerhouse

    SciTech Connect (OSTI)

    Hughes, James S.; Deng, Zhiqun; Weiland, Mark A.; Martinez, Jayson J.; Yuan, Yong

    2011-11-22

    Fish screens at hydroelectric dams help to protect rearing and migrating fish by preventing them from passing through the turbines and directing them towards the bypass channels by providing a sweeping flow parallel to the screen. However, fish screens may actually be harmful to fish if they become impinged on the surface of the screen or become disoriented due to poor flow conditions near the screen. Recent modifications to the vertical barrier screens (VBS) at the Bonneville Dam second powerhouse (B2) intended to increase the guidance of juvenile salmonids into the juvenile bypass system (JBS) have resulted in high mortality and descaling rates of hatchery subyearling Chinook salmon during the 2008 juvenile salmonid passage season. To investigate the potential cause of the high mortality and descaling rates, an in situ water velocity measurement study was conducted using acoustic Doppler velocimeters (ADV) in the gatewell slot at Units 12A and 14A of B2. From the measurements collected the average approach velocity, sweep velocity, and the root mean square (RMS) value of the velocity fluctuations were calculated. The approach velocities measured across the face of the VBS varied but were mostly less than 0.3 m/s. The sweep velocities also showed large variances across the face of the VBS with most measurements being less than 1.5 m/s. This study revealed that the approach velocities exceeded criteria recommended by NOAA Fisheries and Washington State Department of Fish and Wildlife intended to improve fish passage conditions.

  20. Screening the Hanford tanks for trapped gas

    SciTech Connect (OSTI)

    Whitney, P.

    1995-10-01

    The Hanford Site is home to 177 large, underground nuclear waste storage tanks. Hydrogen gas is generated within the waste in these tanks. This document presents the results of a screening of Hanford`s nuclear waste storage tanks for the presence of gas trapped in the waste. The method used for the screening is to look for an inverse correlation between waste level measurements and ambient atmospheric pressure. If the waste level in a tank decreases with an increase in ambient atmospheric pressure, then the compressibility may be attributed to gas trapped within the waste. In this report, this methodology is not used to estimate the volume of gas trapped in the waste. The waste level measurements used in this study were made primarily to monitor the tanks for leaks and intrusions. Four measurement devices are widely used in these tanks. Three of these measure the level of the waste surface. The remaining device measures from within a well embedded in the waste, thereby monitoring the liquid level even if the liquid level is below a dry waste crust. In the past, a steady rise in waste level has been taken as an indicator of trapped gas. This indicator is not part of the screening calculation described in this report; however, a possible explanation for the rise is given by the mathematical relation between atmospheric pressure and waste level used to support the screening calculation. The screening was applied to data from each measurement device in each tank. If any of these data for a single tank indicated trapped gas, that tank was flagged by this screening process. A total of 58 of the 177 Hanford tanks were flagged as containing trapped gas, including 21 of the 25 tanks currently on the flammable gas watch list.

  1. Screening and Evaluation Tool (SET) Users Guide

    SciTech Connect (OSTI)

    Layne Pincock

    2014-10-01

    This document is the users guide to using the Screening and Evaluation Tool (SET). SET is a tool for comparing multiple fuel cycle options against a common set of criteria and metrics. It does this using standard multi-attribute utility decision analysis methods.

  2. This is a special screening of The

    E-Print Network [OSTI]

    This is a special screening of The Dialogue ahead of the film's release in 2014! Watch the trailer, is a 70-minute documentary that follows four American students (one from Michigan State University to deepen their understanding of the "other" and themselves. Although the film is set in Hong Kong and China

  3. Page 1 of 18 Full Screen

    E-Print Network [OSTI]

    Römisch, Werner

    the booking process is controlled by seat protection levels or (so-called) bid prices. Aims: · Development Page 3 of 18 Go Back Full Screen Close Quit Notation Input data n : probability of node n; stochastic,j,k, zd,n i,j,k: slack variables; ~zn i,j,k: auxiliary integer variables; Indices t = 0, . . . , T: data

  4. The ALICE analysis train system

    E-Print Network [OSTI]

    Zimmermann, Markus

    2015-01-01

    In the ALICE experiment hundreds of users are analyzing big datasets on a Grid system. High throughput and short turn-around times are achieved by a centralized system called the LEGO trains. This system combines analysis from different users in so-called analysis trains which are then executed within the same Grid jobs thereby reducing the number of times the data needs to be read from the storage systems. The centralized trains improve the performance, the usability for users and the bookkeeping in comparison to single user analysis. The train system builds upon the already existing ALICE tools, i.e. the analysis framework as well as the Grid submission and monitoring infrastructure. The entry point to the train system is a web interface which is used to configure the analysis and the desired datasets as well as to test and submit the train. Several measures have been implemented to reduce the time a train needs to finish and to increase the CPU efficiency.

  5. The ALICE analysis train system

    E-Print Network [OSTI]

    Markus Zimmermann; for the ALICE collaboration

    2015-02-23

    In the ALICE experiment hundreds of users are analyzing big datasets on a Grid system. High throughput and short turn-around times are achieved by a centralized system called the LEGO trains. This system combines analysis from different users in so-called analysis trains which are then executed within the same Grid jobs thereby reducing the number of times the data needs to be read from the storage systems. The centralized trains improve the performance, the usability for users and the bookkeeping in comparison to single user analysis. The train system builds upon the already existing ALICE tools, i.e. the analysis framework as well as the Grid submission and monitoring infrastructure. The entry point to the train system is a web interface which is used to configure the analysis and the desired datasets as well as to test and submit the train. Several measures have been implemented to reduce the time a train needs to finish and to increase the CPU efficiency.

  6. National Security Science and Technology Initiative: Air Cargo Screening

    SciTech Connect (OSTI)

    Bingham, Philip R [ORNL; White, Tim [Pacific Northwest National Laboratory (PNNL); Cespedes, Ernesto [Idaho National Laboratory (INL); Bowerman, Biays [Brookhaven National Laboratory (BNL); Bush, John [Battelle

    2010-11-01

    The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security's Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with four of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009. The primary results of this effort are described in this document and can be summarized as follows: (1) Completed a gap analysis that identified threat signatures and observables, candidate technologies for detection, their current state of development, and provided recommendations for improvements to meet air cargo screening requirements. (2) Defined a Commodity/Threat/Detection matrix that focuses modeling and experimental efforts, identifies technology gaps and game-changing opportunities, and provides a means of summarizing current and emerging capabilities. (3) Defined key properties (e.g., elemental composition, average density, effective atomic weight) for basic commodity and explosive benchmarks, developed virtual models of the physical distributions (pallets) of three commodity types and three explosive benchmarks combinations, and conducted modeling and simulation studies to begin populating the matrix of commodities, threats, and detection technologies. (4) Designed and fabricated basic (homogeneous) commodity test pallets and fabricated inert stimulants to support experiments and to validate modeling/simulation results. (5) Developed/expanded the team's capabilities to conduct full-scale imaging (neutron and x-ray) experiments of air cargo commodities and explosive benchmarks. (6) Conducted experiments to improve the collection of trace particles of explosives from a variety of surfaces representative of air cargo materials by means of mechanical (air/vibration/pressure), thermal, and electrostatic methods. Air cargo screening is a difficult challenge that will require significant investment in both research and development to find a suitable solution to ensure the safety of passengers without significantly hindering the flow of commodities. The initiative funded by Battelle has positioned this group to make major contributions in meeting the air cargo challenge by developing collaborations, developing laboratory test systems, improving knowledge of the challenges (both technical and business) for air cargo screening, and increasing the understanding of the capabilities for current inspection methods (x-ray radiography, x-ray backscatter, etc.) and potential future inspection methods (neutron radiography, fusion of detector modalities, advanced trace detection, etc.). Lastly, air cargo screening is still an issue that will benefit from collaboration between Department of Energy Laboratories and Battelle. On January 7, 2010, DHS Secretary Napolitano joined White House Press Secretary Robert Gibbs and Assistant to the President for Counterterrorism and Homeland Security John Brennan to announce several recommendations DHS has made to the President for improving the technology and procedures used to protect air travel from acts of terrorism. (This announcement followed the 25 Dec'09 Delta/Northwest Airlines Flight 253 terror attack.) Secretary Napolitano out

  7. A Tomato Detached Leaf Assay for Chemical Genomics of an HLB Model System

    E-Print Network [OSTI]

    Patne, S.; Eulgem, T.; Roose, M. L.

    2014-01-01

    used successfully in many high- throughput chemical screensto identify chemicals inducing defense responses.In our study, we will test chemical uptake and its effect on

  8. Experimental and numerical studies of aerosol penetration through screens 

    E-Print Network [OSTI]

    Han, Tae Won

    2009-05-15

    for one particular type of screen would collapse to a single curve if the collection efficiency is expressed in terms of non-dimensional parameters. Correlations characterizing the aerosol deposition process on different types of screens were developed...

  9. Microfluidic in vivo screen identifies compounds enhancing neuronal

    E-Print Network [OSTI]

    Haggarty, Stephen J.

    Compound screening is a powerful tool to identify new therapeutic targets, drug leads, and elucidate the fundamental mechanisms of biological processes. We report here the results of the first in vivo small-molecule screens ...

  10. HUD CHP GUIDE #2 - FEASIBILITY SCREENING FOR CHP IN MULTIFAMILY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    HUD CHP GUIDE 2 - FEASIBILITY SCREENING FOR CHP IN MULTIFAMILY HOUSING, May 2009 HUD CHP GUIDE 2 - FEASIBILITY SCREENING FOR CHP IN MULTIFAMILY HOUSING, May 2009 The U.S....

  11. Laboratory to change vehicle traffic-screening regimen at vehicle...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Changes to vehicle traffic-screening Laboratory to change vehicle traffic-screening regimen at vehicle inspection station Lanes two through five will be open 24 hours a day and...

  12. Analysis of Assembly Bill 547: Ovarian Cancer Screening

    E-Print Network [OSTI]

    California Health Benefits Review Program (CHBRP)

    2004-01-01

    al. (1999). Screening for ovarian cancer: a pilot randomizedLung, Colorectal & Ovarian Cancer Screening Trial ( P L CA Snapshot of Ovarian Cancer. http://prg.nci.nih.gov/

  13. Development and commercialization of a biomass gasification/power generation system

    SciTech Connect (OSTI)

    Paisley, M.A.; Farris, G.

    1995-11-01

    The US Department of Energy (DOE) has been a leader in the promotion and development of alternative fuel supplies based on renewable energy crops. One promising power generation technology is biomass gasification coupled with either a gas turbine in a combined cycle system or a fuel cell. The gasification of biomass can efficiently and economically produce a renewable source of a clean gaseous fuel suitable for use in these high efficiency power systems or as a substitute fuel in other combustion devices such as boilers, kilns, or other natural gas fired equipment. This paper discusses the development and commercialization of the Battelle high-throughput gasification process for gas turbine based power generation systems. Projected process economics for a gas turbine combined cycle plant are presented along with a description of integrated system operation coupling a 200kW gas turbine power generation system to a 10 ton per day gasifier, and current commercialization activities.

  14. Weakly screened thermonuclear reactions in astrophysical plasmas: Improving Salpeter's model

    E-Print Network [OSTI]

    Theodore E. Liolios

    2003-06-23

    This paper presents a detailed study of the electron degeneracy and nonlinear screening effects which play a crucial role in the validity of Salpeter's weak-screening model. The limitations of that model are investigated and an improved one is proposed which can take into account nonlinear screening effects. Its application to the solar pp reaction derives an accurate screening enhancement factor and provides a very reliable estimation of the associated neutrino flux uncertanties.

  15. MAGNETIC RESONANCE IMAGE VIEWING ``SCREEN REAL ESTATE'' PROBLEM

    E-Print Network [OSTI]

    Atkins, M. Stella

    MAGNETIC RESONANCE IMAGE VIEWING AND THE ``SCREEN REAL ESTATE'' PROBLEM By Johanna van der Heyden B Degree: Master of Science Title of thesis: Magnetic Resonance Image Viewing and the ``Screen Real Estate. These ``screen real estate'' issues are extensively explored in the literature but not consistently applied

  16. Targeted CT Screening for Lung Cancer using Absolute Risk Prediction

    E-Print Network [OSTI]

    Brent, Roger

    Targeted CT Screening for Lung Cancer using Absolute Risk Prediction Stephanie A. Kovalchik skovalch@rand.org FHCRC 2014 Risk Prediction Symposium June 11, 2014 1 #12;Outline · Lung Cancer Epidemiology and Screening · Screening Benefit and Absolute Risk · Absolute Risk Model for Lung Cancer

  17. Screening properties of four mesoscale smoothed charge models, with application to dissipative particle dynamics

    E-Print Network [OSTI]

    Patrick B. Warren; Andrey Vlasov

    2014-02-25

    We extend our previous study [J. Chem. Phys. 138, 204907 (2013)] to quantify the screening properties of four mesoscale smoothed charge models used in dissipative particle dynamics. Using a combination of the hypernetted chain integral equation closure and the random phase approximation, we identify regions where the models exhibit a real-valued screening length, and the extent to which this agrees with the Debye length in the physical system. We find that the second moment of the smoothed charge distribution is a good predictor of this behaviour. We are thus able to recommend a consistent set of parameters for the models.

  18. Nonperturbative screening of the Landau pole

    E-Print Network [OSTI]

    Francesco Giacosa; Ralf Hofmann

    2007-04-19

    Based on the trace anomaly for the energy-momentum tensor, an effective theory for the thermodynamics of the deconfining phase, and by assuming the asymptotic behavior to be determined by one-loop perturbation theory we compute the nonperturbative beta function for the fundamental coupling $g$ in SU(2) and SU(3) Yang-Mills theory. With increasing temperature we observe a very rapid approach to the perturbative running. The Landau pole is nonperturbatively screened.

  19. Screening technology reduces ash in spiral circuits

    SciTech Connect (OSTI)

    Brodzik, P. [Derrick Corp., Buffalo, NY (United States)

    2007-05-15

    In 2006, the James River Coal Co. selected the Stack Sizer to remove the minus 100 mesh high ash clay fraction from the clean coal spiral product circuits at the McCoy-Elkhorn Bevins Branch prep plant and at the Blue Diamond Leatherwood prep plant in Kentucky. The Stack Sizer is a multi-deck, high-frequency vibrating screen capable of separations as fine as 75 microns when fitted with Derrick Corp.'s patented high open area urethane screen panels. Full-scale lab tests and more than 10 months of continuous production have confirmed that the Stack Sizer fitted with Derrick 100 micron urethane screen panels consistently produces a clean coal fraction that ranges from 8 to 10% ash. Currently, each five-deck Stack Sizer operating at the Bevins Branch and Leatherwood prep plants is producing approximately 33 tons per hour of clean coal containing about 9% ash. This represents a clean coal yield of about 75% and an ash reduction of about 11% from the feed slurry. 3 figs. 2 tabs.

  20. SNP Calling Using Genotype Model Selection on High-Throughput Sequencing Data

    E-Print Network [OSTI]

    Murillo, Gabriel Hiroshi

    2012-01-01

    microorganisms or microbes has been shown to be of increasing importance not only for human and domestic animal pathogen

  1. Multi-Channel and Multi-Rate Adaptation for High-Throughput Wireless Networks

    E-Print Network [OSTI]

    Nguyen, Duy Duc

    2012-01-01

    layer. Unlike wired networks, wireless networks have to deal2 Background Wireless Networks Wireless technology is allWLAN), wireless mesh networks, wireless metropolitan area

  2. High-throughput two-hybrid analysis The promise and the peril

    E-Print Network [OSTI]

    Dunham, Maitreya

    , reduction in reagent costs and further refinements of the approach. As throughput escalates, so does use. The first stage is the initial description of a method: the prototype version. Typically. For a large fraction of new technologies, few other examples beyond this proto- type are ever described

  3. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    walls, a small leak, or outgassing from the sample. Theyou have found the leak. Outgassing samples are the mostheld for 24 hrs or until outgassing was determined complete

  4. Algorithms and tools for high-throughput geometry-based analysis...

    Office of Scientific and Technical Information (OSTI)

    (SC-22) Country of Publication: United States Language: English Subject: membrane, carbon capture, materials and chemistry by design, synthesis (novel materials), synthesis...

  5. HIGH-THROUGHPUT/COMBINATORIAL TECHNIQUES IN HYDROGEN STORAGE MATERIALS R&D WORKSHOP

    E-Print Network [OSTI]

    , academia, and national laboratories. The objectives of this meeting were to: · Assess the potential is solution-based with solids handling similar to proven combinatorial methods. Exploration of new structures-ray scattering (SAXS) to determine pore size (needs development), hydrogen uptake me

  6. Development of High Throughput Process for Constructing 454 Titanium and Illumina Libraries

    SciTech Connect (OSTI)

    Deshpande, Shweta; Hack, Christopher; Tang, Eric; Malfatti, Stephanie; Ewing, Aren; Lucas, Susan; Cheng, Jan-Fang

    2010-05-28

    We have developed two processes with the Biomek FX robot to construct 454 titanium and Illumina libraries in order to meet the increasing library demands. All modifications in the library construction steps were made to enable the adaptation of the entire processes to work with the 96-well plate format. The key modifications include the shearing of DNA with Covaris E210 and the enzymatic reaction cleaning and fragment size selection with SPRI beads and magnetic plate holders. The construction of 96 Titanium libraries takes about 8 hours from sheared DNA to ssDNA recovery. The processing of 96 Illumina libraries takes less time than that of the Titanium library process. Although both processes still require manual transfer of plates from robot to other work stations such as thermocyclers, these robotic processes represent about 12- to 24-folds increase of library capacity comparing to the manual processes. To enable the sequencing of many libraries in parallel, we have also developed sets of molecular barcodes for both library types. The requirements for the 454 library barcodes include 10 bases, 40-60percent GC, no consecutive same base, and no less than 3 bases difference between barcodes. We have used 96 of the resulted 270 barcodes to construct libraries and pool to test the ability of accurately assigning reads to the right samples. When allowing 1 base error occurred in the 10 base barcodes, we could assign 99.6percent of the total reads and 100percent of them were uniquely assigned. As for the Illumina barcodes, the requirements include 4 bases, balanced GC, and at least 2 bases difference between barcodes. We have begun to assess the ability to assign reads after pooling different number of libraries. We will discuss the progress and the challenges of these scale-up processes.

  7. High throughput analysis of proteome perturbations induced by radiation, radiomitigators and chemotherapeutics

    E-Print Network [OSTI]

    Erde, Jonathan

    2012-01-01

    in five pathways of glutamate fermentation. Appl MicrobiolH. A. Two pathways of glutamate fermentation by anaerobic

  8. High-throughput film-densitometry: An efficient approach to generate large data sets

    SciTech Connect (OSTI)

    Typke, Dieter; Nordmeyer, Robert A.; Jones, Arthur; Lee, Juyoung; Avila-Sakar, Agustin; Downing, Kenneth H.; Glaeser, Robert M.

    2004-07-14

    A film-handling machine (robot) has been built which can, in conjunction with a commercially available film densitometer, exchange and digitize over 300 electron micrographs per day. Implementation of robotic film handling effectively eliminates the delay and tedium associated with digitizing images when data are initially recorded on photographic film. The modulation transfer function (MTF) of the commercially available densitometer is significantly worse than that of a high-end, scientific microdensitometer. Nevertheless, its signal-to-noise ratio (S/N) is quite excellent, allowing substantial restoration of the output to ''near-to-perfect'' performance. Due to the large area of the standard electron microscope film that can be digitized by the commercial densitometer (up to 10,000 x 13,680 pixels with an appropriately coded holder), automated film digitization offers a fast and inexpensive alternative to high-end CCD cameras as a means of acquiring large amounts of image data in electron microscopy.

  9. Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

    E-Print Network [OSTI]

    Cai, Long

    shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We strength in other fields9. How- ever, SAXS use has sharply increased with advances in synchrotron X,10,11. Notably, SAXS analyses can build on and be combined with other results to test experimental hypotheses

  10. Scalable Computational Methods for the Analysis of High-Throughput Biological Data

    SciTech Connect (OSTI)

    Langston, Michael A

    2012-09-06

    This primary focus of this research project is elucidating genetic regulatory mechanisms that control an organism�¢����s responses to low-dose ionizing radiation. Although low doses (at most ten centigrays) are not lethal to humans, they elicit a highly complex physiological response, with the ultimate outcome in terms of risk to human health unknown. The tools of molecular biology and computational science will be harnessed to study coordinated changes in gene expression that orchestrate the mechanisms a cell uses to manage the radiation stimulus. High performance implementations of novel algorithms that exploit the principles of fixed-parameter tractability will be used to extract gene sets suggestive of co-regulation. Genomic mining will be performed to scrutinize, winnow and highlight the most promising gene sets for more detailed investigation. The overall goal is to increase our understanding of the health risks associated with exposures to low levels of radiation.

  11. High-throughput, single-worm tracking and analysis in Caenorhabditis elegans

    E-Print Network [OSTI]

    Yemini, Eviatar

    2013-01-08

    orifices at the mouth, anus, and, for hermaphrodites, at the vulva as well. Males have special mating adaptations at their tail. At low magnification, as would be expected, C. elegans have a simple worm-like morphology and the tail has a slightly sharper... shapes (as defined by bending angles along the worm body) 15. The most salient and commonly explored behavior in C. elegans is locomotion. Locomotion is powered by 95 muscles separated into four longitudinal quadrants 16. These quadrants represent left...

  12. Discovery of BRD4 bromodomain inhibitors by fragment-based high-throughput docking

    E-Print Network [OSTI]

    Caflisch, Amedeo

    % as only 24 putative inhibitors, from an initial library of about 9 million molecules, were tested in vitro efficiency (LE), defined as the ratio of the free energy of binding to the number of non-hydrogen atoms.11 Previously, we have developed a fragment-based in silico proce- dure called ALTA (Anchor-based Library

  13. Development and Application of a High Throughput Methodology to Characterize and Formulate Protein-Based Therapeutics

    E-Print Network [OSTI]

    Hu, Lei

    2012-12-31

    concentration and excipients on stability, as well as their effects on solubility.8 Although not addressed in the present work, other studies including photostability, agitation, and freeze-thaw cycling are also usually performed to evaluate the effect...

  14. Evaluation of a New Remote Handling Design for High Throughput Annular Centrifugal Contactors

    SciTech Connect (OSTI)

    David H. Meikrantz; Troy G. Garn; Jack D. Law; Lawrence L. Macaluso

    2009-09-01

    Advanced designs of nuclear fuel recycling plants are expected to include more ambitious goals for aqueous based separations including; higher separations efficiency, high-level waste minimization, and a greater focus on continuous processes to minimize cost and footprint. Therefore, Annular Centrifugal Contactors (ACCs) are destined to play a more important role for such future processing schemes. Previous efforts defined and characterized the performance of commercial 5 cm and 12.5 cm single-stage ACCs in a “cold” environment. The next logical step, the design and evaluation of remote capable pilot scale ACCs in a “hot” or radioactive environment was reported earlier. This report includes the development of remote designs for ACCs that can process the large throughput rates needed in future nuclear fuel recycling plants. Novel designs were developed for the remote interconnection of contactor units, clean-in-place and drain connections, and a new solids removal collection chamber. A three stage, 12.5 cm diameter rotor module has been constructed and evaluated for operational function and remote handling in highly radioactive environments. This design is scalable to commercial CINC ACC models from V-05 to V-20 with total throughput rates ranging from 20 to 650 liters per minute. The V-05R three stage prototype was manufactured by the commercial vendor for ACCs in the U.S., CINC mfg. It employs three standard V-05 clean-in-place (CIP) units modified for remote service and replacement via new methods of connection for solution inlets, outlets, drain and CIP. Hydraulic testing and functional checks were successfully conducted and then the prototype was evaluated for remote handling and maintenance suitability. Removal and replacement of the center position V-05R ACC unit in the three stage prototype was demonstrated using an overhead rail mounted PaR manipulator. This evaluation confirmed the efficacy of this innovative design for interconnecting and cleaning individual stages while retaining the benefits of commercially reliable ACC equipment for remote applications in the nuclear industry. Minor modifications and suggestions for improved manual remote servicing by the remote handling specialists were provided but successful removal and replacement was demonstrated in the first prototype.

  15. Droplet microfluidics for high-throughput biological assays Mira T. Guo,a

    E-Print Network [OSTI]

    developed over the past several decades have greatly impacted medical research, therapeutics signal-to-noise ratios restrict the possible targets of medical diagnostics, and thus limit their medical sensitivities by using water-in-oil emulsion droplets to compartmentalize reactants into picolitre volumes,3

  16. Inch-Scale High Throughput Metrology of Graphene and Patterned Graphene Oxide

    E-Print Network [OSTI]

    Pleskot, Dennis

    2013-01-01

    Strength of Monolayer Graphene Science 2008 321, 385-388. 5.Novoselov, K. S. The rise of graphene Nature 2007 6 183-191.A. Thermal properties of graphene and nanostructured carbon

  17. AFLOW: An automatic framework for high-throughput materials discovery Stefano Curtarolo a,b,

    E-Print Network [OSTI]

    Curtarolo, Stefano

    , Denis O. Demchenko f , Dane Morgan g a Department of Mechanical Engineering and Materials Science, Duke Commonwealth University, Richmond, VA 23284, United States g Department of Materials Science and Engineering-optimization techniques used for the study of high-temperature reactions in multicomponent hydrides [29­31]. In its

  18. Analysis of Genomic Rearrangements in Cancer from High Throughput Sequencing Data

    E-Print Network [OSTI]

    Ballinger, Tracy Jean

    2015-01-01

    Breakage fusion bridge (BFB) cycles are thought to play achromosome, a combination of BFB and chromothripsis events,they are not explainable by BFB cycles or chromothripsis

  19. High-throughput single-molecule fluorescence spectroscopy using parallel detection

    E-Print Network [OSTI]

    Michalet, Xavier

    -molecule imaging). In this section, we briefly discuss the technique and data analysis used in standard solution-based , S. Tisad , F. Zappad , S. Covad , S. Weissa a Dept of Chemistry & Biochemistry, Los Angeles, CA, USA 90095; b Nesher Technologies, Los Angeles, CA; c Dept of Physics & Astronomy, UCLA, Los Angeles, CA

  20. Multi-Channel and Multi-Rate Adaptation for High-Throughput Wireless Networks

    E-Print Network [OSTI]

    Nguyen, Duy Duc

    2012-01-01

    collisions, and natural radio waves phenomena. This isphenomena associated to radio wave propagation are the keyinformation bit into radio waves. The wireless medium is

  1. High-Throughput, High-Precision Hot Testing Tool for High-Brightness...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Diode Testing Lead Performer: KLA-Tencor Corporation - Milpitas, CA Partners: Ocean Optics - Dunedin, FL DOE Total Funding: 3,994,729 Cost Share: 4,626,422 Project Term: 815...

  2. The Development of a High-throughput Microdroplet Bioreactor Device for Microbial Studies 

    E-Print Network [OSTI]

    Guzman, Adrian

    2012-10-19

    . These methods often produce much larger forces that are capable of overcoming the additional surface stability added by surfactant usage. Single target droplet merging can be accomplished using a laser in a variety of ways including cavitational bubble... induction, thermal localized heating, or surface convective heating. In the case of cavitational bubble induction, a bubble containing the vapor of the dispersed phase is induced using a localized laser heating source [43]. The droplet then expands...

  3. Ultra-high Throughput Real-time Instruments for Capturing Fast Signals and Rare Events

    E-Print Network [OSTI]

    Buckley, Brandon Walter

    2013-01-01

    using a syringe pump, at a fixed volumetric rate. The fluidat a fixed volumetric rate using a syringe pump. CHAPTER 9

  4. High-throughput microfluidic living cell arrays for spatiotemporal gene expression profiling

    E-Print Network [OSTI]

    King, Kevin R. (Kevin Robert), 1976-

    2008-01-01

    The cellular microenvironment is remarkably complex. In the small space near each cell, growth factors are liberated from extracellular matrix, cytokines are secreted from neighboring cells, and hormones arrive from distant ...

  5. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    rheology through microfluidics,” Advanced Materials, vol.Berthier and P. Silberzan, Microfluidics for Biotechnology.A. P. Lee, “Droplet microfluidics,” Lab on a Chip, vol. 8,

  6. Bayesian Model Selection for High-dimensional High-throughput Data 

    E-Print Network [OSTI]

    Joshi, Adarsh

    2012-07-16

    , the 10 g-prior corresponds to an alternative distribution of the following form: H ?2 : ? ? N(0, c n ). (2.5) Hence the expression for the corresponding Bayes? factor can be written as BF (2|1) = ?(Y? , 0, (c + 1)/n) ?(Y? , 0, 1/n) = 1 (c + 1... to the interval (a, a) by MoM prior centered on 0 with scale ? and n = 1 is 2 [ ? ( a ? ? ) ? a ? 2pi? exp ( ? a2 2? ) ? 1 2 ] . (2.21) 17 For an iMoM prior, the corresponding probability is 1?G [ (a/ ? ?)?2k; ? 2k , 1 ] , (2...

  7. High throughput analysis of proteome perturbations induced by radiation, radiomitigators and chemotherapeutics

    E-Print Network [OSTI]

    Erde, Jonathan

    2012-01-01

    research and biohydrogen production development. Chapters 6,fossil fuel-free biohydrogen production, and for proteomicbe important for biohydrogen production 1 . It is capable of

  8. High throughput 3D optical microscopy : from image cytometry to endomicroscopy

    E-Print Network [OSTI]

    Choi, Heejin

    2014-01-01

    Optical microscopy is an imaging technique that allows morphological mapping of intracellular structures with submicron resolution. More importantly, optical microscopy is a technique that can readily provide images with ...

  9. Treatability studies on different refinery wastewater samples using high-throughput microbial electrolysis cells (MECs)

    E-Print Network [OSTI]

    , 2005), brewery (Feng et al., 2008), animal (Min et al., 2005) and paper recycling wastewaters (Huang

  10. High-throughput, dual probe biological assays based on single molecule detection

    DOE Patents [OSTI]

    Hollars, Christopher W. (Brentwood, CA); Huser, Thomas R. (Livermore, CA); Lane, Stephen M. (Oakland, CA); Balhorn, Rodney L. (Livermore, CA); Bakajin, Olgica (San Leandro, CA); Darrow, Christopher (Pleasanton, CA); Satcher, Jr., Joe H. (Patterson, CA)

    2006-07-11

    A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 .mu.m.times.100 .mu.m.times.100 .mu.m. A device using these methods would have 10.sup.3 10.sup.4 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 .mu.m.sup.3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (.about.10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

  11. Development and operation of a high-throughput accurate-wavelength...

    Office of Scientific and Technical Information (OSTI)

    imaging. A precision optical encoder measures the grating angle with an accuracy 0.075 arc sec. A high quantum efficiency low-etaloning CCD detector allows operation at longer...

  12. SUPPLEMENTARY DATA DNAshape: a method for the high-throughput prediction

    E-Print Network [OSTI]

    Rohs, Remo

    central base pairs (underlined in Supplementary Table S2), and (C-D) comparisons with crystal structures 3 in Figure 2 include data from X-ray crystallography (1bna, 2bna, 355d, 428d, 455d, 1dou, 1fq2, and 1jgr

  13. High-Throughput Automated Luminescent Magnetic Particle-Based Immunoassay to Monitor

    E-Print Network [OSTI]

    Hammock, Bruce D.

    to Pyrethroid Insecticides Ki Chang Ahn, Pete Lohstroh,,# Shirley J. Gee, Nancy A. Gee, Bill Lasley, and Bruce D, University of California, Davis, California 95616 We have developed a sensitive, automated, competitive

  14. High throughput analysis of proteome perturbations induced by radiation, radiomitigators and chemotherapeutics

    E-Print Network [OSTI]

    Erde, Jonathan

    2012-01-01

    expression levels in Normal LCLs, 24 hours after treatmenthuman lymphoblastoid cell lines (LCLs) exposed to ionizingresponse components in Normal LCLs, while thrombin signaling

  15. Geochip: A high throughput genomic tool for linking community structure to functions

    E-Print Network [OSTI]

    Van Nostrand, Joy D.

    2009-01-01

    mentioned previously, probe design criteria are important inis determining probe design criteria, as this will greatlyof oligonucleotide probe design criteria. Appl Environ

  16. Ultra-high Throughput Real-time Instruments for Capturing Fast Signals and Rare Events

    E-Print Network [OSTI]

    Buckley, Brandon Walter

    2013-01-01

    fiber; OSA: Optical spectrum analyzer; PD: Photo-detector;high-speed optical spectrum analyzer [60]. We call this1% tap on an optical spectrum analyzer (OSA). The resulting

  17. Inertio-elastic focusing of bioparticles in a microchannel at ultra-high throughput

    E-Print Network [OSTI]

    Lim, Eugene J. (Eugene Jungsud), 1980-

    2014-01-01

    Many biological and industrial fluids are filled with micro-scale particles that can serve as "state markers" for real-world issues, such as human health and public infrastructure. In order to extract this valuable information ...

  18. Applications of high throughput (combinatorial) methodologies to electronic, magnetic, optical, and energy-related materials

    E-Print Network [OSTI]

    Rubloff, Gary W.

    , and energy-related materials Martin L. Green, Ichiro Takeuchi, and Jason R. Hattrick-Simpers Citation: J) methodologies to electronic, magnetic, optical, and energy-related materials Martin L. Green,1 Ichiro Takeuchi,2 materials science for electronic, magnetic, optical, and energy-related materials. It is expected that high

  19. Marrying microfluidics and microwells for parallel, high-throughput single-cell genomics

    E-Print Network [OSTI]

    Hughes, Travis K.

    An innovative, microwell-based platform for single-cell RNA sequencing (RNA-seq) combines cost efficiency, scalability and parallelizability, and will enable many new avenues of biological inquiry.

  20. High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries

    E-Print Network [OSTI]

    Rohde, Christopher Benjamin

    Therapeutic treatment of spinal cord injuries, brain trauma, stroke, and neurodegenerative diseases will greatly benefit from the discovery of compounds that enhance neuronal regeneration following injury. We previously ...

  1. Lensfree Optical Tomography for High-Throughput 3D Imaging on a Chip

    E-Print Network [OSTI]

    ISIKMAN, SERHAN OMER

    2012-01-01

    microscope with light-emitting diode illumination. Opt.source such as a light emitting diode (LED). The fundamentalchip microscope, 24 light-emitting diodes (LEDs - each with

  2. High Throughput Ab initio Modeling of Charge Transport for Bio-Molecular-Electronics

    E-Print Network [OSTI]

    Bruque, Nicolas A.

    2009-01-01

    DNA-Based Molecular Electronics. vol. 725. New York: AIP,Heath, “Whence molecular electronics? ” Science, vol. 306,model,” J. Computational Electronics, vol. 6, no. 4, pp.

  3. WHAM: A High-throughput Sequence Alignment Method Yinan Li Allison Terrell Jignesh M. Patel

    E-Print Network [OSTI]

    Willett, Rebecca

    -1-4503-0661-4/11/06 ...$10.00. only a small portion (called a "read") of the genome. The human genome project delivered its anniversary of the sequencing of the first human genome [1, 21]. This key scientific discovery has been to drug discovery and drug design for personal- ized medicine. A crucial part of this first human genome

  4. SNP Calling Using Genotype Model Selection on High-Throughput Sequencing Data

    E-Print Network [OSTI]

    Murillo, Gabriel Hiroshi

    2012-01-01

    Institute. The human genome project comple- tion: Frequentlycommunity, the Human Genome Project (HGP) was finished inmain goals of the human genome project was to create a human

  5. High-throughput gene expression profiling of memory differentiation in primary human T cells

    E-Print Network [OSTI]

    Angelosanto, Jill

    Background: The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and ...

  6. High-throughput experimental and computational tools for exploring immunity and the microbiome

    E-Print Network [OSTI]

    Papa, Eliseo

    2012-01-01

    Humans live in association with trillions of microbes and yet we know remarkably little about their symbiotic relationship. The role these microorganisms have in humans has been characterized only in the case of few bacteria ...

  7. Editor's Choice The AFLOW standard for high-throughput materials science calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    c , Michael J. Mehl d , Gus Hart e , Marco Buongiorno Nardelli f , Stefano Curtarolo g, a Department calculation and communication protocols. Following these guidelines would promote optimal use of the results

  8. Development of a high throughput 3D perfused liver tissue bioreactor

    E-Print Network [OSTI]

    Inman, Samuel Walker

    2006-01-01

    This thesis describes the development of a device designed for culturing liver tissue in a 3D perfused environment. Cells form tissue inside miniature channels of a scaffold, and the tissue is perfused with culture medium ...

  9. High-throughput approaches to sourcing of human hepatocytes for cell-based therapies

    E-Print Network [OSTI]

    Shan, Jing (Jing Meghan)

    2014-01-01

    Chronic liver disease affects more than 500 million people worldwide. The only therapy shown to directly prevent mortality is organ transplantation. However, there is growing discrepancy between supply and demand of ...

  10. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    3.1.2 Benefits of Thermoplastic Devices . . . . . . . . .temperatures for four thermoplastics. The pressure in eachto fabricate and test thermoplastic devices is presented.

  11. Supplemental Information Assessing the thermoelectric properties of sintered compounds via high-throughput ab initio calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    6277 orc oP12 12 0.50 1.59 1.82 6.00 0.54 0.38 0.47 0.77 4.28 0.09 4.76 7.98 Ag1I1 43435 cub cP2 2 0.04 2.17 2.19 2.94 2.47 3.10 3.25 Ag2Se1 15213 orc oP12 12 0.16 1.12 0.30 0.60 0.14 0.10 0.12 0.21 5.08 0.08 0.37 0.37 0.37 0.37 Al1As3Ca3 32727 orc oP28 28 0.72 1.89 0.84 1.23 1.72 0.32 0.94 3.89 2.06 1

  12. High throughput parallel backside contacting and periodic texturing for high-efficiency solar cells

    DOE Patents [OSTI]

    Daniel, Claus; Blue, Craig A.; Ott, Ronald D.

    2014-08-19

    Disclosed are configurations of long-range ordered features of solar cell materials, and methods for forming same. Some features include electrical access openings through a backing layer to a photovoltaic material in the solar cell. Some features include textured features disposed adjacent a surface of a solar cell material. Typically the long-range ordered features are formed by ablating the solar cell material with a laser interference pattern from at least two laser beams.

  13. Operations capability improvement of a molecular biology laboratory in a high throughput genome sequencing center

    E-Print Network [OSTI]

    Vokoun, Matthew R. (Matthew Richard)

    2005-01-01

    The Broad Institute is a research collaboration of MIT, Harvard University and affiliated hospitals, and the Whitehead Institute for Biomedical Research. Its scientific mission is to "(1) create tools for genomic medicine ...

  14. SUPPLEMENTAL INFORMATION Treatability of Complex Effluents in High-Throughput and Bench Scale Microbial

    E-Print Network [OSTI]

    energy based on measured current, applied potential, and COD removal with various substrates in mini. The efficiency was calculated using only recovered hydrogen and combined hydrogen and methane, using the heat of combustion to calculate the energy contained in the gas. Figure S13. Open circuit gas production and COD

  15. An approach to simultaneously test multiple devices for high-throughput production of thin film electronics

    E-Print Network [OSTI]

    Kumar, A.; Flewitt, A. J.

    2015-07-28

    of transparent flexible thin-film transistors using amorphous oxide semiconductors.,” Nature, vol. 432, no. 7016, pp. 488–492, 2004. [2] E. Fortunato, P. Barquinha, and R. Martins, “Oxide semiconductor thin-film transistors: a review of recent advances... , M. Lavine, and R. Coontz, “Looking Beyond Silicon,” Science, vol. 327, no. 5973. pp. 1595–1595, 2010. [6] A. J. Flewitt, “Hydrogenated Amorphous Silicon Thin Film Transistors (a Si:H TFTs),” Handbook of Visual Display Technology-Springer, 2012...

  16. High-Throughput Single-Cell Manipulation in Brain Joseph D. Steinmeyer1

    E-Print Network [OSTI]

    Yanik, Mehmet Fatih

    . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which Award (R01 NS066352) and a Packard Award in Science and Engineering. J.D.S. was supported

  17. Some Clustering and Classification Problems in High-Throughput Metagenomics and Cheminformatics

    E-Print Network [OSTI]

    Tanaseichuk, Olga

    2013-01-01

    Gut Microbiome [43] and Acid Mine Drainage Biofilm [104].obtained from the acid mine drainage [104]. This dataset has

  18. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    of com- plex oil inlet shapes. (a) 2D extrusion to form aof complex oil inlet shapes. (a) 2D extrusion to form a pad.

  19. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    is a valid assumption. The viscosity of the oil was alsoassumption is often wrong, be- Table 4.2: Summary of measured viscosities from capillary measurement. Oil/

  20. High-throughput and long-term observation of compartmentalized biochemical oscillators

    E-Print Network [OSTI]

    Koshi Hasatani; Mathieu Leocmach; Anthony J. Genot; André Estévez-Torres; Teruo Fujii; Yannick Rondelez

    2015-11-04

    We report the splitting of an oscillating DNA circuit into $\\sim 700$ droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenomena - slow desynchronization between the compartments and kinematic spatial waves - and investigate their possible origin. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.

  1. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    tension versus time plot of peanut oil with varying weightshowing autofluorescence of peanut oil/SPAN80 mixture. Thetension versus time plot of peanut oil with varying weight

  2. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    3.5.1 Molding Attempts . . . . . . . . . . . . . . . . .3.8 Injection Molding of futureW. K. Schomburg, “Review on micro molding of ther- moplastic

  3. Multi-Channel and Multi-Rate Adaptation for High-Throughput Wireless Networks

    E-Print Network [OSTI]

    Nguyen, Duy Duc

    2012-01-01

    experiments include wireless cards and router with Atherosexperiments include wireless cards and router with Atherosvia wired or wireless connection to AP router. In both

  4. Analysis of Genomic Rearrangements in Cancer from High Throughput Sequencing Data

    E-Print Network [OSTI]

    Ballinger, Tracy Jean

    2015-01-01

    2.2 Cancer evolution . . . . . . . . . . . . . . . . . . . .5 Retrotransposition in cancer genomes 5.1 Retrotransposonrearrangements in cancer . . . . . . . . 2.3.2 Graph-based

  5. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    gas storage1.2 Compressed gas storage Technology is already well-of the compressed gas storage method is that it has been

  6. Supplementary Materials: The AFLOW Standard for High-Throughput Materials Science Calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    , [Cu4ACHTUNGTRENNUNG(Me3CCOO)8ACHTUNGTRENNUNG(teia)]·7.5PrOH (SNU-80a), were pre- pared by the reaction-crystal X-ray diffraction study of SNU-80a reveals that each teia coordinates four Cu2+ ions of four [Cu2 by PLATON.[11] The theoretical surface area of the framework of SNU-80a is 1440 m2 gŔ1 as estimated

  7. Encoded hydrogel microparticles for high-throughput molecular diagnostics and personalized medicine

    E-Print Network [OSTI]

    Chapin, Stephen Clifford

    2012-01-01

    The ability to accurately detect and quantify biological molecules in complex mixtures is crucial in basic research as well as in clinical settings. Advancements in genetic analysis, molecular diagnostics, and patient-tailored ...

  8. Examination of mammalian microRNAs by high-throughput sequencing

    E-Print Network [OSTI]

    Chiang, HyoJin Rosaria

    2011-01-01

    Small non-coding RNAs play an important role in a wide range of cellular events. MicroRNAs (miRNAs) are an abundant class of small RNAs that post-transcriptionally repress expression of their target genes. Since miRNA ...

  9. Antibody-functionalized nanoporous surfaces enable high throughput specific cell capture

    E-Print Network [OSTI]

    Mittal, Sukant

    2012-01-01

    Adhesion-based cell capture on surfaces in microfluidic devices forms the basis of numerous biomedical diagnostics and in vitro assays. Solid surface microfluidic platforms have been widely explored for biomedical diagnostics ...

  10. Polymers with hydro-responsive topography identified using high throughput AFM of an acrylate microarray

    E-Print Network [OSTI]

    Hook, Andrew L.

    Atomic force microscopy has been applied to an acrylate polymer microarray to achieve a full topographic characterisation. This process discovered a small number of hydro-responsive materials created from monomers with ...

  11. Air-stable ink for scalable, high-throughput layer deposition

    DOE Patents [OSTI]

    Weil, Benjamin D; Connor, Stephen T; Cui, Yi

    2014-02-11

    A method for producing and depositing air-stable, easily decomposable, vulcanized ink on any of a wide range of substrates is disclosed. The ink enables high-volume production of optoelectronic and/or electronic devices using scalable production methods, such as roll-to-roll transfer, fast rolling processes, and the like.

  12. Development and implementation of high-throughput SNP genotyping in barley

    E-Print Network [OSTI]

    2009-01-01

    USA, 6 Department of Crop and Soil Science, Oregon State9 Department of Crop and Soil Sciences, Washington StateSciences, University of California (UCR), Riverside, CA, 92521, USA, 3 Scottish Crop

  13. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    palladium (Pd) to protect them from oxidation as well as to enhance hydrogenpalladium or nickel. These materials also offer an aid studies by catalyzing hydrogen

  14. High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform

    E-Print Network [OSTI]

    Abalde-Cela, Sara; Gould, Anna; Liu, Xin; Kazamia, Elena; Smith, Alison G.; Abell, Chris

    2015-03-17

    . Both negative controls show essen- lation of cells. Those containing BG11 7. Azevedo AM, Prazeres DMF, Cabral JMS, Fonseca LP. 2005 Ethanol biosensors based on alcohol oxidase. Biosens. Bioelectron. 21, 235–247. (doi:10.1016/j. bios.2004.09.030) 8...

  15. High Throughput Isolation and Glycosylation Analysis of IgGVariability and Heritability of

    E-Print Network [OSTI]

    ., Glycobiology Division, Planinska 1, 10000 Zagreb, Croatia; §BIA Separations Ltd., Teslova 30, 1000 Ljubljana School of Medicine, S oltanska 2, Split, Croatia; **University of Za- greb, Faculty of Pharmacy and Biochemistry, Ante Kovac ic´ a 1, 10000 Zagreb, Croatia; Oxford Glycobiology Institute, Department of Bio

  16. High throughput low power decoder architectures for low density parity check codes 

    E-Print Network [OSTI]

    Selvarathinam, Anand Manivannan

    2005-11-01

    ?????????????????..?... 14 3 Iteration Logic Stage???????????????????..?... 15 4 Fully Parallel Architecture?????????????????..?.. 17 5 BER/FER Simulation Result for Different Values of M?????.??. 29 6 Iteration Stage ? Combinational Logic Scaling... Patterns?????? 46 11 H Matrix for M = N/12 and 10, 12 Patterns????????????.. 46 12 BER Performance for M = N/6 and Different Patterns????????. 49 13 FER Performance for M = N/6 and Different Patterns????????. 49 14 BER Performance for M = N/12...

  17. High-Throughput, High-Precision Hot Testing Tool for High-Brightness

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing Tool Fits the Bill Financing ToolSustainableSecurityHighDepartment ofLight-Emitting Diode

  18. High-Throughput/Combinatorial Techniques in Hydrogen Storage Materials R&D

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing Tool Fits the Bill Financing ToolSustainableSecurityHighDepartment ofLight-Emitting

  19. Attendees list from the U.S. Department of Energy's High Throughput

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based Fuels Research atDepartment ofAtlanta

  20. Biochemical & Thermochemical High Throughput Characterization of Feedstocks for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based Fuels Researchof Energy|