National Library of Energy BETA

Sample records for high throughput tools

  1. High-Throughput, High-Precision Hot Testing Tool for High-Brightness...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput, High-Precision Hot Testing Tool for High-Brightness Light-Emitting Diode Testing Lead Performer: KLA-Tencor Corporation - Milpitas, CA Partners: Ocean Optics - ...

  2. High-Throughput, High-Precision Hot Testing Tool for High-Brightness

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Light-Emitting Diode Testing | Department of Energy Throughput, High-Precision Hot Testing Tool for High-Brightness Light-Emitting Diode Testing High-Throughput, High-Precision Hot Testing Tool for High-Brightness Light-Emitting Diode Testing Lead Performer: KLA-Tencor Corporation - Milpitas, CA Partners: Ocean Optics - Dunedin, FL DOE Total Funding: $3,994,729 Cost Share: $4,626,422 Project Term: 8/15/2012 - 9/30/2015 Funding Opportunity: SSL Manufacturing R&D Funding Opportunity

  3. Algorithms and tools for high-throughput geometry-based analysis...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algorithms and tools for high-throughput geometry-based analysis of crystalline porous materials Previous Next List Thomas F. Willems, Chris H. Rycroft, Michaeel Kazi, Juan C....

  4. Algorithms and tools for high-throughput geometry-based analysis...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Algorithms and tools for high-throughput geometry-based analysis of crystalline porous materials ... Research Org: Energy Frontier Research Centers (EFRC); ...

  5. Express Primer Tool for high-throughput gene cloning and expression

    Energy Science and Technology Software Center (OSTI)

    2002-12-01

    A tool to assist in the design of primers for DNA amplification. The Express Primer web-based tool generates primer sequences specifically for the generation of expression clones for both lab scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for simultaneous amplification of multiple target genes.more » The Express Primer Tool enables the user to specify various experimental parameters (e.g. optimal Tm, Tm range, maximum Tm difference) for single or multiple candidate sequence(s) in FASTA format input as a flat text (ASCII) file. The application generates condidate primers, selects optimal primer pairs, and writes the forward and reverse primers pairs to an Excel file that is suitable for electronic submission to a synthesis facility. The program parameters emphasize high-throughput but allow for target atrition at various stages of the project.« less

  6. Protein Sequence Annotation Tool (PSAT): A centralized web-based meta-server for high-throughput sequence annotations

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Leung, Elo; Huang, Amy; Cadag, Eithon; Montana, Aldrin; Soliman, Jan Lorenz; Zhou, Carol L. Ecale

    2016-01-20

    In this study, we introduce the Protein Sequence Annotation Tool (PSAT), a web-based, sequence annotation meta-server for performing integrated, high-throughput, genome-wide sequence analyses. Our goals in building PSAT were to (1) create an extensible platform for integration of multiple sequence-based bioinformatics tools, (2) enable functional annotations and enzyme predictions over large input protein fasta data sets, and (3) provide a web interface for convenient execution of the tools. In this paper, we demonstrate the utility of PSAT by annotating the predicted peptide gene products of Herbaspirillum sp. strain RV1423, importing the results of PSAT into EC2KEGG, and using the resultingmore » functional comparisons to identify a putative catabolic pathway, thereby distinguishing RV1423 from a well annotated Herbaspirillum species. This analysis demonstrates that high-throughput enzyme predictions, provided by PSAT processing, can be used to identify metabolic potential in an otherwise poorly annotated genome. Lastly, PSAT is a meta server that combines the results from several sequence-based annotation and function prediction codes, and is available at http://psat.llnl.gov/psat/. PSAT stands apart from other sequencebased genome annotation systems in providing a high-throughput platform for rapid de novo enzyme predictions and sequence annotations over large input protein sequence data sets in FASTA. PSAT is most appropriately applied in annotation of large protein FASTA sets that may or may not be associated with a single genome.« less

  7. High throughput optical scanner

    DOE Patents [OSTI]

    Basiji, David A. (Seattle, WA); van den Engh, Gerrit J. (Seattle, WA)

    2001-01-01

    A scanning apparatus is provided to obtain automated, rapid and sensitive scanning of substrate fluorescence, optical density or phosphorescence. The scanner uses a constant path length optical train, which enables the combination of a moving beam for high speed scanning with phase-sensitive detection for noise reduction, comprising a light source, a scanning mirror to receive light from the light source and sweep it across a steering mirror, a steering mirror to receive light from the scanning mirror and reflect it to the substrate, whereby it is swept across the substrate along a scan arc, and a photodetector to receive emitted or scattered light from the substrate, wherein the optical path length from the light source to the photodetector is substantially constant throughout the sweep across the substrate. The optical train can further include a waveguide or mirror to collect emitted or scattered light from the substrate and direct it to the photodetector. For phase-sensitive detection the light source is intensity modulated and the detector is connected to phase-sensitive detection electronics. A scanner using a substrate translator is also provided. For two dimensional imaging the substrate is translated in one dimension while the scanning mirror scans the beam in a second dimension. For a high throughput scanner, stacks of substrates are loaded onto a conveyor belt from a tray feeder.

  8. Automated High Throughput Drug Target Crystallography

    SciTech Connect (OSTI)

    Rupp, B

    2005-02-18

    The molecular structures of drug target proteins and receptors form the basis for 'rational' or structure guided drug design. The majority of target structures are experimentally determined by protein X-ray crystallography, which as evolved into a highly automated, high throughput drug discovery and screening tool. Process automation has accelerated tasks from parallel protein expression, fully automated crystallization, and rapid data collection to highly efficient structure determination methods. A thoroughly designed automation technology platform supported by a powerful informatics infrastructure forms the basis for optimal workflow implementation and the data mining and analysis tools to generate new leads from experimental protein drug target structures.

  9. High throughput protein production screening

    DOE Patents [OSTI]

    Beernink, Peter T.; Coleman, Matthew A.; Segelke, Brent W.

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  10. NETL Studies High Throughput Membrane Screening | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NETL Studies High Throughput Membrane Screening NETL Studies High Throughput Membrane Screening Membranes offer a potential low-maintenance and economical method for gas ...

  11. Potential of High-Throughput Experimentation with Ammonia Borane...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of High-Throughput Experimentation with Ammonia Borane Solid Hydrogen Storage Materials (presentation) Potential of High-Throughput Experimentation with Ammonia Borane Solid ...

  12. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D (presentation) High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D (presentation)...

  13. High Throughput Materials Characterization John M. Gregoire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Paper for Establishing a User Facility for Synchrotron-based High Throughput Materials Characterization John M. Gregoire 1 , Matthew J. Kramer 2 , Apurva Mehta 3 1 Joint Center for Artificial Photosynthesis, California Institute of Technology, Pasadena, CA, gregoire@caltech.edu 2 Critial Materials Institute, Ames Laboratory, Iowa State University, Ames IA, mjkramer@ameslab.gov 3 Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA,

  14. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Environmental Management (EM)

    High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D On June 26, 2007 the Hydrogen ...

  15. High throughput chemical munitions treatment system

    DOE Patents [OSTI]

    Haroldsen, Brent L.; Stofleth, Jerome H.; Didlake, Jr., John E.; Wu, Benjamin C-P

    2011-11-01

    A new High-Throughput Explosive Destruction System is disclosed. The new system is comprised of two side-by-side detonation containment vessels each comprising first and second halves that feed into a single agent treatment vessel. Both detonation containment vessels further comprise a surrounding ventilation facility. Moreover, the detonation containment vessels are designed to separate into two half-shells, wherein one shell can be moved axially away from the fixed, second half for ease of access and loading. The vessels are closed by means of a surrounding, clam-shell type locking seal mechanisms.

  16. High Throughput Combinatorial Screening of Biometic Metal-Organic...

    Broader source: Energy.gov (indexed) [DOE]

    miaminrelparilla.pdf (1.38 MB) More Documents & Publications High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint ...

  17. High Throughput/Combinatorial Screening of Hydrogen Storage Materials (presentation)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop HIGH THROUGHPUT/COMBINATORIAL SCREENING OF HYDROGEN STORAGE MATERIALS June 26, 2007 Tom Boussie Symyx Technologies Symyx develops and applies proprietary high-throughput research technologies and software to increase R&D efficiency in chemical, energy, electronics, pharmaceutical and academic labs. * Pioneer of High Throughput Research (HTR) for materials science * Founded in 1996; publicly traded since 1999 (SMMX: NASDAQ) * 400 Employees (mainly in Santa Clara, CA) * >$400

  18. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that...

  19. A targeted proteomics toolkit for high-throughput absolute quantificat...

    Office of Scientific and Technical Information (OSTI)

    targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins Citation Details In-Document Search Title: A targeted proteomics toolkit for ...

  20. High-throughput metagenomic technologies for complex microbial...

    Office of Scientific and Technical Information (OSTI)

    formats Prev Next Title: High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats You are accessing a document from...

  1. High Throughput/Combinatorial Screening of Hydrogen Storage Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Presentation by Adriaan Sachtler from the High Throughput Combinatorial Analysis of Hydrogen Storage Materials Meeting PDF icon sachtler.pdf More Documents & Publications ...

  2. High-Throughput Analytical Model to Evaluate Materials for Temperature...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    High-Throughput Analytical Model to Evaluate Materials for Temperature Swing Adsorption Processes Previous Next List mcontent.jpg Julian P. Sculley, Wolfgang M. Verdegaal, Weigang...

  3. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that require genomic-scale information, such as Berkeley Lab's bioenergy efforts and cancer biology studies. Artist's abstract depiction of high-throughput SAXS combining...

  4. High-throughput metagenomic technologies for complex microbial...

    Office of Scientific and Technical Information (OSTI)

    high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e.g., phylogenetic and functional gene arrays). Here, we provide a...

  5. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Robust, High-Throughput Analysis of Protein Structures Print Wednesday, 28 October 2009 00:00 Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling

  6. High Throughput/Combinatorial Screening of Hydrogen Storage Materials...

    Broader source: Energy.gov (indexed) [DOE]

    High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint UC-BerkeleySymyx DoDDLA Project) (presentation) High Througput ...

  7. High Throughput/Combinatorial Screening of Hydrogen Storage Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (presentation) | Department of Energy Materials (presentation) High Throughput/Combinatorial Screening of Hydrogen Storage Materials (presentation) Presented at the U.S. Department of Energy's Hydrogen Storage Meeting held June 26, 2007 in Bethesda, Maryland. ht_symyx_boussie.pdf (1013.19 KB) More Documents & Publications High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint UC-Berkeley/Symyx DoD/DLA Project) (presentation) High

  8. Interactive Visual Analysis of High Throughput Text Streams

    SciTech Connect (OSTI)

    Steed, Chad A; Potok, Thomas E; Patton, Robert M; Goodall, John R; Maness, Christopher S; Senter, James K

    2012-01-01

    The scale, velocity, and dynamic nature of large scale social media systems like Twitter demand a new set of visual analytics techniques that support near real-time situational awareness. Social media systems are credited with escalating social protest during recent large scale riots. Virtual communities form rapidly in these online systems, and they occasionally foster violence and unrest which is conveyed in the users language. Techniques for analyzing broad trends over these networks or reconstructing conversations within small groups have been demonstrated in recent years, but state-of- the-art tools are inadequate at supporting near real-time analysis of these high throughput streams of unstructured information. In this paper, we present an adaptive system to discover and interactively explore these virtual networks, as well as detect sentiment, highlight change, and discover spatio- temporal patterns.

  9. High-Throughput Characterization of Porous Materials Using Graphics...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    High-Throughput Characterization of Porous Materials Using Graphics Processing Units Previous Next List J. Kim, R. L. Martin, O. Rubel, M. Haranczyk, and B. Smit, J. Chem. Theory...

  10. SAMDI Mass Spectrometry for High Throughput Discovery of Enzyme...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SAMDI Mass Spectrometry for High Throughput Discovery of Enzyme Function January 15, 2016 11:00AM to 12:00PM Presenter Milan Mrksich, Northwestern University Location Building 446,...

  11. Final Report - High throughput CIGS solar cell fabrication via...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High throughput CIGS solar cell fabrication via ultra-thin absorber layer with optical ... Contact Materials for Improved Performance CdTe Solar Cells Download the SunShot ...

  12. Extended length microchannels for high density high throughput electrophoresis systems

    DOE Patents [OSTI]

    Davidson, James C.; Balch, Joseph W.

    2000-01-01

    High throughput electrophoresis systems which provide extended well-to-read distances on smaller substrates, thus compacting the overall systems. The electrophoresis systems utilize a high density array of microchannels for electrophoresis analysis with extended read lengths. The microchannel geometry can be used individually or in conjunction to increase the effective length of a separation channel while minimally impacting the packing density of channels. One embodiment uses sinusoidal microchannels, while another embodiment uses plural microchannels interconnected by a via. The extended channel systems can be applied to virtually any type of channel confined chromatography.

  13. Accelerating Electrolyte Discovery for Energy Storage with High Throughput

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Screening - Joint Center for Energy Storage Research December 26, 2014, Research Highlights Accelerating Electrolyte Discovery for Energy Storage with High Throughput Screening A screening scheme has been developed to down-select molecule candidates based on successive property evaluations obtained from high-throughput computations. Here we show the down-select results for ~1400 candidates for non-aqueous redox flow battery application. Scientific Achievement We have developed a strategy to

  14. NETL Studies High Throughput Membrane Screening | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NETL Studies High Throughput Membrane Screening NETL Studies High Throughput Membrane Screening Membranes offer a potential low-maintenance and economical method for gas separations from power plant flue gas streams. Polymer membranes and supported liquid membranes show great promise to solve problems in the area of clean energy production. Carbon dioxide, a greenhouse gas, is a principal by-product of energy production from fossil fuels. Capturing CO2 from power plant flue gas streams is

  15. High-Throughput Experimental Approach Capabilities | Materials Science |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL High-Throughput Experimental Approach Capabilities An image of a triangular diagram with cobalt oxide at the top vertex, zinc oxide at the lower left vertex, and nickel oxide at the lower right vertex. Colored section in upper half indicates conductivity of materials at constant oxygen partial pressure and temperature. Highest conductivity is represented by yellow and is for materials in the upper right sector. NREL's high-throughput experimental approach is based on the extensive set

  16. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  17. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  18. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  19. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  20. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the ALS has implemented the world's highest-throughput biological-solution x-ray scattering beamline enabling genomic-scale protein-structure characterization. Coupling brilliant x rays from one of the

  1. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches

    Broader source: Energy.gov [DOE]

    Presentation by Adriaan Sachtler from the High Throughput/ Combinatorial Analysis of Hydrogen Storage Materials Meeting

  2. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7 UOP LLC. All rights reserved. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP Approaches High Throughput/Combinatorial Analysis of Hydrogen Storage Materials Meeting Organized by DOE on June 26, 2007 Adriaan Sachtler High Throughput/Combinatorial Analysis of Hydrogen Storage Materials Meeting Organized by DOE on June 26, 2007 Adriaan Sachtler © 2007 UOP LLC. All rights reserved. 2

  3. Adaptive Sampling for High Throughput Data Using Similarity Measures

    SciTech Connect (OSTI)

    Bulaevskaya, V.; Sales, A. P.

    2015-05-06

    The need for adaptive sampling arises in the context of high throughput data because the rates of data arrival are many orders of magnitude larger than the rates at which they can be analyzed. A very fast decision must therefore be made regarding the value of each incoming observation and its inclusion in the analysis. In this report we discuss one approach to adaptive sampling, based on the new data point’s similarity to the other data points being considered for inclusion. We present preliminary results for one real and one synthetic data set.

  4. Environmental characterization studies of a high-throughput wood gasifier

    SciTech Connect (OSTI)

    Chang, H.; Niemann, R.C.; Wilzbach, K.E.; Paisley, M.

    1983-01-01

    Potential environmental effects associated with thermochemical biomass gasification have been studied by Argonne National Laboratory in cooperation with Battelle Columbus Laboratories (BCL). A series of samples from the process research unit of an indirectly heated, high-throughput wood gasifier operated by BCL has been analyzed for potentially toxic organic compounds and trace elements. The results indicate that, under the test-run conditions, the gasification of both pine and hardwood is accompanied by the formation of some oil, the heavier fraction of which gives a positive response in the Ames assay for mutagenicity and contains numerous phenols and polycyclic aromatic hydrocarbons, including some carcinogens. The implications of these observations are discussed.

  5. Computational Proteomics: High-throughput Analysis for Systems Biology

    SciTech Connect (OSTI)

    Cannon, William R.; Webb-Robertson, Bobbie-Jo M.

    2007-01-03

    High-throughput (HTP) proteomics is a rapidly developing field that offers the global profiling of proteins from a biological system. The HTP technological advances are fueling a revolution in biology, enabling analyses at the scales of entire systems (e.g., whole cells, tumors, or environmental communities). However, simply identifying the proteins in a cell is insufficient for understanding the underlying complexity and operating mechanisms of the overall system. Systems level investigations are relying more and more on computational analyses, especially in the field of proteomics generating large-scale global data.

  6. Agenda from the U.S. Department of Energy's High Throughput Screening...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Screening of Hydrogen Storage Materials Workshop on June 26, 2007 High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint ...

  7. High-Throughput Methodology for Discovery of Metal-Organic Frameworks...

    Broader source: Energy.gov (indexed) [DOE]

    High Throughput Combinatorial Screening of Biometic Metal-Organic Materials for Military Hydrogen-Storage Materials (New Joint Miami UNREL DoDDLA Project) (presentation) High ...

  8. High-Throughput/Combinatorial Techniques in Hydrogen Storage Materials R&D

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy High-Throughput/Combinatorial Techniques in Hydrogen Storage Materials R&D High-Throughput/Combinatorial Techniques in Hydrogen Storage Materials R&D On June 26, 2007 the Hydrogen Storage Program of the U.S. Department of Energy (DOE) held a one-day meeting to identify how to better implement high-throughput/combinatorial techniques to benefit challenging research on advanced hydrogen storage materials. Participants represented industry, academia, and National

  9. High Throughput Pretreatment and Enzyme Hydrolysis of Biomass: Screening Recalcitrance in Large Sample Populations (Presentation)

    SciTech Connect (OSTI)

    Decker, S. R.

    2010-10-01

    Presentation on the execution of the first high-throughput thermochemical pretreatment/enzyme digestion pipeline for screening biomass for recalcitrance.

  10. Generalized schemes for high throughput manipulation of the Desulfovibrio vulgaris Hildenborough genome

    SciTech Connect (OSTI)

    Chhabra, S.R.; Butland, G.; Elias, D.; Chandonia, J.-M.; Fok, V.; Juba, T.; Gorur, A.; Allen, S.; Leung, C.-M.; Keller, K.; Reveco, S.; Zane, G.; Semkiw, E.; Prathapam, R.; Gold, B.; Singer, M.; Ouellet, M.; Sazakal, E.; Jorgens, D.; Price, M.; Witkowska, E.; Beller, H.; Hazen, T.C.; Biggin, M.; Auer, M.; Wall, J.; Keasling, J.

    2011-07-15

    The ability to conduct advanced functional genomic studies of the thousands of sequenced bacteria has been hampered by the lack of available tools for making high- throughput chromosomal manipulations in a systematic manner that can be applied across diverse species. In this work, we highlight the use of synthetic biological tools to assemble custom suicide vectors with reusable and interchangeable DNA “parts” to facilitate chromosomal modification at designated loci. These constructs enable an array of downstream applications including gene replacement and creation of gene fusions with affinity purification or localization tags. We employed this approach to engineer chromosomal modifications in a bacterium that has previously proven difficult to manipulate genetically, Desulfovibrio vulgaris Hildenborough, to generate a library of over 700 strains. Furthermore, we demonstrate how these modifications can be used for examining metabolic pathways, protein-protein interactions, and protein localization. The ubiquity of suicide constructs in gene replacement throughout biology suggests that this approach can be applied to engineer a broad range of species for a diverse array of systems biological applications and is amenable to high-throughput implementation.

  11. Advances in High Throughput Screening of Biomass Recalcitrance (Poster)

    SciTech Connect (OSTI)

    Turner, G. B.; Decker, S. R.; Tucker, M. P.; Law, C.; Doeppke, C.; Sykes, R. W.; Davis, M. F.; Ziebell, A.

    2012-06-01

    This was a poster displayed at the Symposium. Advances on previous high throughput screening of biomass recalcitrance methods have resulted in improved conversion and replicate precision. Changes in plate reactor metallurgy, improved preparation of control biomass, species-specific pretreatment conditions, and enzymatic hydrolysis parameters have reduced overall coefficients of variation to an average of 6% for sample replicates. These method changes have improved plate-to-plate variation of control biomass recalcitrance and improved confidence in sugar release differences between samples. With smaller errors plant researchers can have a higher degree of assurance more low recalcitrance candidates can be identified. Significant changes in plate reactor, control biomass preparation, pretreatment conditions and enzyme have significantly reduced sample and control replicate variability. Reactor plate metallurgy significantly impacts sugar release aluminum leaching into reaction during pretreatment degrades sugars and inhibits enzyme activity. Removal of starch and extractives significantly decreases control biomass variability. New enzyme formulations give more consistent and higher conversion levels, however required re-optimization for switchgrass. Pretreatment time and temperature (severity) should be adjusted to specific biomass types i.e. woody vs. herbaceous. Desalting of enzyme preps to remove low molecular weight stabilizers and improved conversion levels likely due to water activity impacts on enzyme structure and substrate interactions not attempted here due to need to continually desalt and validate precise enzyme concentration and activity.

  12. Solion ion source for high-efficiency, high-throughput solar cell manufacturing

    SciTech Connect (OSTI)

    Koo, John Binns, Brant; Miller, Timothy; Krause, Stephen; Skinner, Wesley; Mullin, James

    2014-02-15

    In this paper, we introduce the Solion ion source for high-throughput solar cell doping. As the source power is increased to enable higher throughput, negative effects degrade the lifetime of the plasma chamber and the extraction electrodes. In order to improve efficiency, we have explored a wide range of electron energies and determined the conditions which best suit production. To extend the lifetime of the source we have developed an in situ cleaning method using only existing hardware. With these combinations, source life-times of >200 h for phosphorous and >100 h for boron ion beams have been achieved while maintaining 1100 cell-per-hour production.

  13. Towards Chip Scale Liquid Chromatography and High Throughput Immunosensing

    SciTech Connect (OSTI)

    Ni, J.

    2000-09-21

    This work describes several research projects aimed towards developing new instruments and novel methods for high throughput chemical and biological analysis. Approaches are taken in two directions. The first direction takes advantage of well-established semiconductor fabrication techniques and applies them to miniaturize instruments that are workhorses in analytical laboratories. Specifically, the first part of this work focused on the development of micropumps and microvalves for controlled fluid delivery. The mechanism of these micropumps and microvalves relies on the electrochemically-induced surface tension change at a mercury/electrolyte interface. A miniaturized flow injection analysis device was integrated and flow injection analyses were demonstrated. In the second part of this work, microfluidic chips were also designed, fabricated, and tested. Separations of two fluorescent dyes were demonstrated in microfabricated channels, based on an open-tubular liquid chromatography (OT LC) or an electrochemically-modulated liquid chromatography (EMLC) format. A reduction in instrument size can potentially increase analysis speed, and allow exceedingly small amounts of sample to be analyzed under diverse separation conditions. The second direction explores the surface enhanced Raman spectroscopy (SERS) as a signal transduction method for immunoassay analysis. It takes advantage of the improved detection sensitivity as a result of surface enhancement on colloidal gold, the narrow width of Raman band, and the stability of Raman scattering signals to distinguish several different species simultaneously without exploiting spatially-separated addresses on a biochip. By labeling gold nanoparticles with different Raman reporters in conjunction with different detection antibodies, a simultaneous detection of a dual-analyte immunoassay was demonstrated. Using this scheme for quantitative analysis was also studied and preliminary dose-response curves from an immunoassay of a

  14. Low inlet gas velocity high throughput biomass gasifier

    DOE Patents [OSTI]

    Feldmann, Herman F.; Paisley, Mark A.

    1989-01-01

    The present invention discloses a novel method of operating a gasifier for production of fuel gas from carbonaceous fuels. The process disclosed enables operating in an entrained mode using inlet gas velocities of less than 7 feet per second, feedstock throughputs exceeding 4000 lbs/ft.sup.2 -hr, and pressures below 100 psia.

  15. A Class of Allosteric Caspase Inhibitors Identified by High-Throughput...

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Search Results Journal Article: A Class of Allosteric Caspase Inhibitors Identified by High-Throughput Screening Citation Details In-Document Search Title: A Class ...

  16. Attendees list from the U.S. Department of Energy's High Throughput...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Attendees list from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Attendees list from the U.S. Department of ...

  17. Improved Algae-based Biorefining and High-throughput Screening of Algal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Photosynthetic Efficiency - Energy Innovation Portal Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Improved Algae-based Biorefining and High-throughput Screening of Algal Photosynthetic Efficiency University of Colorado Contact CU About This Technology Publications: PDF Document Publication CU2807B (Biorefining Flow Cytometer) Marketing Summary.pdf (164 KB) Technology Marketing Summary Improved Algae-based Biorefining and High-throughput Screening of Algal

  18. High-throughput analysis of T-DNA location and structure using sequence capture

    SciTech Connect (OSTI)

    Inagaki, Soichi; Henry, Isabelle M.; Lieberman, Meric C.; Comai, Luca

    2015-10-07

    Agrobacterium-mediated transformation of plants with T-DNA is used both to introduce transgenes and for mutagenesis. Conventional approaches used to identify the genomic location and the structure of the inserted T-DNA are laborious and high-throughput methods using next-generation sequencing are being developed to address these problems. Here, we present a cost-effective approach that uses sequence capture targeted to the T-DNA borders to select genomic DNA fragments containing T-DNA—genome junctions, followed by Illumina sequencing to determine the location and junction structure of T-DNA insertions. Multiple probes can be mixed so that transgenic lines transformed with different T-DNA types can be processed simultaneously, using a simple, index-based pooling approach. We also developed a simple bioinformatic tool to find sequence read pairs that span the junction between the genome and T-DNA or any foreign DNA. We analyzed 29 transgenic lines of Arabidopsis thaliana, each containing inserts from 4 different T-DNA vectors. We determined the location of T-DNA insertions in 22 lines, 4 of which carried multiple insertion sites. Additionally, our analysis uncovered a high frequency of unconventional and complex T-DNA insertions, highlighting the needs for high-throughput methods for T-DNA localization and structural characterization. Transgene insertion events have to be fully characterized prior to use as commercial products. As a result, our method greatly facilitates the first step of this characterization of transgenic plants by providing an efficient screen for the selection of promising lines.

  19. High-throughput analysis of T-DNA location and structure using sequence capture

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Inagaki, Soichi; Henry, Isabelle M.; Lieberman, Meric C.; Comai, Luca

    2015-10-07

    Agrobacterium-mediated transformation of plants with T-DNA is used both to introduce transgenes and for mutagenesis. Conventional approaches used to identify the genomic location and the structure of the inserted T-DNA are laborious and high-throughput methods using next-generation sequencing are being developed to address these problems. Here, we present a cost-effective approach that uses sequence capture targeted to the T-DNA borders to select genomic DNA fragments containing T-DNA—genome junctions, followed by Illumina sequencing to determine the location and junction structure of T-DNA insertions. Multiple probes can be mixed so that transgenic lines transformed with different T-DNA types can be processed simultaneously,more » using a simple, index-based pooling approach. We also developed a simple bioinformatic tool to find sequence read pairs that span the junction between the genome and T-DNA or any foreign DNA. We analyzed 29 transgenic lines of Arabidopsis thaliana, each containing inserts from 4 different T-DNA vectors. We determined the location of T-DNA insertions in 22 lines, 4 of which carried multiple insertion sites. Additionally, our analysis uncovered a high frequency of unconventional and complex T-DNA insertions, highlighting the needs for high-throughput methods for T-DNA localization and structural characterization. Transgene insertion events have to be fully characterized prior to use as commercial products. As a result, our method greatly facilitates the first step of this characterization of transgenic plants by providing an efficient screen for the selection of promising lines.« less

  20. Low inlet gas velocity high throughput biomass gasifier

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.

    1989-05-09

    A method is described for operating a gasifier which comprises: introducing inlet gas at a velocity of about 0.5 to 7 ft/sec to fluidize a bed in a gasifier vessel; forming the bed into a fluidized bed in a first space region by means of the inlet gas, the fluidized bed containing a circulating hot relatively fine and inert solid bed particle component; inputting and throughputting carbonaceous material into and through the first space region with fluidized bed at a rate from 500-4400 lbs/ft/sup 2/-hr; endothermally pyrolyzing the carbonaceous material by means of the circulating hot inert particle component so as to form a product gas; forming contiguous to and above the fluidized bed a lower average density entrained space region containing an entrained mixture of inert solid particles, char, and carbonaceous material and the product gas; gradually and continuously removing the entrained mixture and the product gas from the lower average density entrained space region of the gasifier to a separator, residence time of the carbonaceous material in the gasifier not exceeding 3 minutes on average; separating the entrained mixture from the product gas; passing the entrained mixture containing inert solid particles, char, and carbonaceous material through an exothermic reaction zone to add heat; and returning at least the inert solid particles to the first space region.

  1. High throughput screening of ligand binding to macromolecules using high resolution powder diffraction

    DOE Patents [OSTI]

    Von Dreele, Robert B.; D'Amico, Kevin

    2006-10-31

    A process is provided for the high throughput screening of binding of ligands to macromolecules using high resolution powder diffraction data including producing a first sample slurry of a selected polycrystalline macromolecule material and a solvent, producing a second sample slurry of a selected polycrystalline macromolecule material, one or more ligands and the solvent, obtaining a high resolution powder diffraction pattern on each of said first sample slurry and the second sample slurry, and, comparing the high resolution powder diffraction pattern of the first sample slurry and the high resolution powder diffraction pattern of the second sample slurry whereby a difference in the high resolution powder diffraction patterns of the first sample slurry and the second sample slurry provides a positive indication for the formation of a complex between the selected polycrystalline macromolecule material and at least one of the one or more ligands.

  2. Agenda from the U.S. Department of Energy's High Throughput Screening of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Storage Materials Workshop on June 26, 2007 | Department of Energy from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Agenda from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Agenda from the U.S. Department of Energy's Hydrogen Storage Meeting held June 26, 2007 in Bethesda, Maryland. ht_agenda.pdf (85.29 KB) More Documents & Publications

  3. High-Throughput Program for the Discovery of NOx Reduction Catalysts |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy High-Throughput Program for the Discovery of NOx Reduction Catalysts High-Throughput Program for the Discovery of NOx Reduction Catalysts 2004 Diesel Engine Emissions Reduction (DEER) Conference Presentation: General Motors Corporation 2004_deer_blint.pdf (357.96 KB) More Documents & Publications WA_02_042_GENERAL_MOTORS_POWER_TRAIN_DIV_Waiver_of_Domestic_.pdf Heavy-Duty NOx Emissions Control: Reformer-Assisted vs. Plasma-Facilitated Lean NOx Catalysis Lean-NOx

  4. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Hantke, Max, F.

    2014-11-17

    Preprocessed detector images that were used for the paper "High-throughput imaging of heterogeneous cell organelles with an X-ray laser". The CXI file contains the entire recorded data - including both hits and blanks. It also includes down-sampled images and LCLS machine parameters. Additionally, the Cheetah configuration file is attached that was used to create the pre-processed data.

  5. Low Cost Lithography Tool for High Brightness LED Manufacturing

    SciTech Connect (OSTI)

    Andrew Hawryluk; Emily True

    2012-06-30

    The objective of this activity was to address the need for improved manufacturing tools for LEDs. Improvements include lower cost (both capital equipment cost reductions and cost-ofownership reductions), better automation and better yields. To meet the DOE objective of $1- 2/kilolumen, it will be necessary to develop these highly automated manufacturing tools. Lithography is used extensively in the fabrication of high-brightness LEDs, but the tools used to date are not scalable to high-volume manufacturing. This activity addressed the LED lithography process. During R&D and low volume manufacturing, most LED companies use contact-printers. However, several industries have shown that these printers are incompatible with high volume manufacturing and the LED industry needs to evolve to projection steppers. The need for projection lithography tools for LED manufacturing is identified in the Solid State Lighting Manufacturing Roadmap Draft, June 2009. The Roadmap states that Projection tools are needed by 2011. This work will modify a stepper, originally designed for semiconductor manufacturing, for use in LED manufacturing. This work addresses improvements to yield, material handling, automation and throughput for LED manufacturing while reducing the capital equipment cost.

  6. Melter Throughput Enhancements for High-Iron HLW

    SciTech Connect (OSTI)

    Kruger, A. A.; Gan, Hoa; Joseph, Innocent; Pegg, Ian L.; Matlack, Keith S.; Chaudhuri, Malabika; Kot, Wing

    2012-12-26

    This report describes work performed to develop and test new glass and feed formulations in order to increase glass melting rates in high waste loading glass formulations for HLW with high concentrations of iron. Testing was designed to identify glass and melter feed formulations that optimize waste loading and waste processing rate while meeting all processing and product quality requirements. The work included preparation and characterization of crucible melts to assess melt rate using a vertical gradient furnace system and to develop new formulations with enhanced melt rate. Testing evaluated the effects of waste loading on glass properties and the maximum waste loading that can be achieved. The results from crucible-scale testing supported subsequent DuraMelter 100 (DM100) tests designed to examine the effects of enhanced glass and feed formulations on waste processing rate and product quality. The DM100 was selected as the platform for these tests due to its extensive previous use in processing rate determination for various HLW streams and glass compositions.

  7. Automated High Throughput Protein Crystallization Screening at Nanoliter Scale and Protein Structural Study on Lactate Dehydrogenase

    SciTech Connect (OSTI)

    Fenglei Li

    2006-08-09

    The purposes of our research were: (1) To develop an economical, easy to use, automated, high throughput system for large scale protein crystallization screening. (2) To develop a new protein crystallization method with high screening efficiency, low protein consumption and complete compatibility with high throughput screening system. (3) To determine the structure of lactate dehydrogenase complexed with NADH by x-ray protein crystallography to study its inherent structural properties. Firstly, we demonstrated large scale protein crystallization screening can be performed in a high throughput manner with low cost, easy operation. The overall system integrates liquid dispensing, crystallization and detection and serves as a whole solution to protein crystallization screening. The system can dispense protein and multiple different precipitants in nanoliter scale and in parallel. A new detection scheme, native fluorescence, has been developed in this system to form a two-detector system with a visible light detector for detecting protein crystallization screening results. This detection scheme has capability of eliminating common false positives by distinguishing protein crystals from inorganic crystals in a high throughput and non-destructive manner. The entire system from liquid dispensing, crystallization to crystal detection is essentially parallel, high throughput and compatible with automation. The system was successfully demonstrated by lysozyme crystallization screening. Secondly, we developed a new crystallization method with high screening efficiency, low protein consumption and compatibility with automation and high throughput. In this crystallization method, a gas permeable membrane is employed to achieve the gentle evaporation required by protein crystallization. Protein consumption is significantly reduced to nanoliter scale for each condition and thus permits exploring more conditions in a phase diagram for given amount of protein. In addition

  8. High-Throughput Plasmid cDNA Library Screening

    SciTech Connect (OSTI)

    Wan, Kenneth H.; Yu, Charles; George, Reed A.; Carlson, JosephW.; Hoskins, Roger A.; Svirskas, Robert; Stapleton, Mark; Celniker, SusanE.

    2006-05-24

    Libraries of cDNA clones are valuable resources foranalysing the expression, structure, and regulation of genes, as well asfor studying protein functions and interactions. Full-length cDNA clonesprovide information about intron and exon structures, splice junctionsand 5'- and 3'-untranslated regions (UTRs). Open reading frames (ORFs)derived from cDNA clones can be used to generate constructs allowingexpression of native proteins and N- or C-terminally tagged proteins.Thus, obtaining full-length cDNA clones and sequences for most or allgenes in an organism is critical for understanding genome functions.Expressed sequence tag (EST) sequencing samples cDNA libraries at random,which is most useful at the beginning of large-scale screening projects.However, as projects progress towards completion, the probability ofidentifying unique cDNAs via EST sequencing diminishes, resulting in poorrecovery of rare transcripts. We describe an adapted, high-throughputprotocol intended for recovery of specific, full-length clones fromplasmid cDNA libraries in five days.

  9. Uranium Transport in a High-Throughput Electrorefiner for EBR-II Blanket Fuel

    SciTech Connect (OSTI)

    Ahluwalia, Rajesh K.; Hua, Thanh Q.; Vaden, DeeEarl

    2004-01-15

    A unique high-throughput Mk-V electrorefiner is being used in the electrometallurgical treatment of the metallic sodium-bonded blanket fuel from the Experimental Breeder Reactor II. Over many cycles, it transports uranium back and forth between the anodic fuel dissolution baskets and the cathode tubes until, because of imperfect adherence of the dendrites, it all ends up in the product collector at the bottom. The transport behavior of uranium in the high-throughput electrorefiner can be understood in terms of the sticking coefficients for uranium adherence to the cathode tubes in the forward direction and to the dissolution baskets in the reverse direction. The sticking coefficients are inferred from the experimental voltage and current traces and are correlated in terms of a single parameter representing the ratio of the cell current to the limiting current at the surface acting as the cathode. The correlations are incorporated into an engineering model that calculates the transport of uranium in the different modes of operation. The model also uses the experimentally derived electrorefiner operating maps that describe the relationship between the cell voltage and the cell current for the three principal transport modes. It is shown that the model correctly simulates the cycle-to-cycle variation of the voltage and current profiles. The model is used to conduct a parametric study of electrorefiner throughput rate as a function of the principal operating parameters. The throughput rate is found to improve with lowering of the basket rotation speed, reduction of UCl{sub 3} concentration in salt, and increasing the maximum cell current or cut-off voltage. Operating conditions are identified that can improve the throughput rate by 60 to 70% over that achieved at present.

  10. High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats

    SciTech Connect (OSTI)

    Zhou, Jizhong; He, Zhili; Yang, Yunfeng; Deng, Ye; Tringe, Susannah G.; Alvarez-Cohen, Lisa

    2015-01-27

    Understanding the structure, functions, activities and dynamics of microbial communities in natural environments is one of the grand challenges of 21st century science. To address this challenge, over the past decade, numerous technologies have been developed for interrogating microbial communities, of which some are amenable to exploratory work (e.g., high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e.g., phylogenetic and functional gene arrays). Here, we provide a critical review and synthesis of the most commonly applied “open-format” and “closed-format” detection technologies. We discuss their characteristics, advantages, and disadvantages within the context of environmental applications and focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference genomes are few. In addition, we discuss crucial issues and considerations associated with applying complementary high-throughput molecular technologies to address important ecological questions.

  11. A versatile toolkit for high throughput functional genomics with Trichoderma reesei

    SciTech Connect (OSTI)

    Schuster, Andre; Bruno, Kenneth S.; Collett, James R.; Baker, Scott E.; Seiboth, Bernhard; Kubicek, Christian P.; Schmoll, Monika

    2012-01-02

    The ascomycete fungus, Trichoderma reesei (anamorph of Hypocrea jecorina), represents a biotechnological workhorse and is currently one of the most proficient cellulase producers. While strain improvement was traditionally accomplished by random mutagenesis, a detailed understanding of cellulase regulation can only be gained using recombinant technologies. RESULTS: Aiming at high efficiency and high throughput methods, we present here a construction kit for gene knock out in T. reesei. We provide a primer database for gene deletion using the pyr4, amdS and hph selection markers. For high throughput generation of gene knock outs, we constructed vectors using yeast mediated recombination and then transformed a T. reesei strain deficient in non-homologous end joining (NHEJ) by spore electroporation. This NHEJ-defect was subsequently removed by crossing of mutants with a sexually competent strain derived from the parental strain, QM9414.CONCLUSIONS:Using this strategy and the materials provided, high throughput gene deletion in T. reesei becomes feasible. Moreover, with the application of sexual development, the NHEJ-defect can be removed efficiently and without the need for additional selection markers. The same advantages apply for the construction of multiple mutants by crossing of strains with different gene deletions, which is now possible with considerably less hands-on time and minimal screening effort compared to a transformation approach. Consequently this toolkit can considerably boost research towards efficient exploitation of the resources of T. reesei for cellulase expression and hence second generation biofuel production.

  12. High throughput lessons from the LHC experience.Johnston.TNC2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    high throughput in widely distributed data management and analysis systems: Lessons from the LHC William E. Johnston ESnet, Lawrence Berkeley National Laboratory e-mail: wej@es.net Eli Dart ESnet, Lawrence Berkeley National Laboratory e-mail: dart@es.net Michael Ernst RHIC and ATLAS Computing Facility, Brookhaven National Laboratory e-mail: mernst@bnl.gov Brian Tierney ESnet, Lawrence Berkeley National Laboratory e-mail: bltierney@es.net Paper type Technical paper Abstract Today's large-scale

  13. Improving network performance on multicore systems: Impact of core affinities on high throughput flows

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Future Generation Computer Systems ( ) - Contents lists available at ScienceDirect Future Generation Computer Systems journal homepage: www.elsevier.com/locate/fgcs Improving network performance on multicore systems: Impact of core affinities on high throughput flows Nathan Hanford a,∗ , Vishal Ahuja a , Matthew Farrens a , Dipak Ghosal a , Mehmet Balman b , Eric Pouyoul b , Brian Tierney b a Department of Computer Science, University of California, Davis, CA, United States b Energy Sciences

  14. Baculovirus expression system and method for high throughput expression of genetic material

    DOE Patents [OSTI]

    Clark, Robin; Davies, Anthony

    2001-01-01

    The present invention provides novel recombinant baculovirus expression systems for expressing foreign genetic material in a host cell. Such expression systems are readily adapted to an automated method for expression foreign genetic material in a high throughput manner. In other aspects, the present invention features a novel automated method for determining the function of foreign genetic material by transfecting the same into a host by way of the recombinant baculovirus expression systems according to the present invention.

  15. Potential of High-Throughput Experimentation with Ammonia Borane Solid Hydrogen Storage Materials (presentation)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of High-Throughput Experimentation with Ammonia Borane Solid Hydrogen Storage Materials Jonathan L. Male Pacific Northwest National Laboratory June 26, 2006 US Department of Energy Energy Efficiency and Renewable Energy (Chemical) Hydrogen Storage DOE EERE Chemical Hydrogen Center * Controlling release of hydrogen from NH 3 BH 3 - Regeneration of NH 3 BH 3 - Engineering, experiment and theory - Materials Discovery DOE BES Hydrogen Fuel Initiative * Structure and dynamics (Neutron and NMR) -

  16. A new fungal large subunit ribosomal RNA primer for high throughput sequencing surveys

    SciTech Connect (OSTI)

    Mueller, Rebecca C.; Gallegos-Graves, La Verne; Kuske, Cheryl R.

    2015-12-09

    The inclusion of phylogenetic metrics in community ecology has provided insights into important ecological processes, particularly when combined with high-throughput sequencing methods; however, these approaches have not been widely used in studies of fungal communities relative to other microbial groups. Two obstacles have been considered: (1) the internal transcribed spacer (ITS) region has limited utility for constructing phylogenies and (2) most PCR primers that target the large subunit (LSU) ribosomal unit generate amplicons that exceed current limits of high-throughput sequencing platforms. We designed and tested a PCR primer (LR22R) to target approximately 300–400 bp region of the D2 hypervariable region of the fungal LSU for use with the Illumina MiSeq platform. Both in silico and empirical analyses showed that the LR22R–LR3 pair captured a broad range of fungal taxonomic groups with a small fraction of non-fungal groups. Phylogenetic placement of publically available LSU D2 sequences showed broad agreement with taxonomic classification. Comparisons of the LSU D2 and the ITS2 ribosomal regions from environmental samples and known communities showed similar discriminatory abilities of the two primer sets. Altogether, these findings show that the LR22R–LR3 primer pair has utility for phylogenetic analyses of fungal communities using high-throughput sequencing methods.

  17. A new fungal large subunit ribosomal RNA primer for high throughput sequencing surveys

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Mueller, Rebecca C.; Gallegos-Graves, La Verne; Kuske, Cheryl R.

    2015-12-09

    The inclusion of phylogenetic metrics in community ecology has provided insights into important ecological processes, particularly when combined with high-throughput sequencing methods; however, these approaches have not been widely used in studies of fungal communities relative to other microbial groups. Two obstacles have been considered: (1) the internal transcribed spacer (ITS) region has limited utility for constructing phylogenies and (2) most PCR primers that target the large subunit (LSU) ribosomal unit generate amplicons that exceed current limits of high-throughput sequencing platforms. We designed and tested a PCR primer (LR22R) to target approximately 300–400 bp region of the D2 hypervariable regionmore » of the fungal LSU for use with the Illumina MiSeq platform. Both in silico and empirical analyses showed that the LR22R–LR3 pair captured a broad range of fungal taxonomic groups with a small fraction of non-fungal groups. Phylogenetic placement of publically available LSU D2 sequences showed broad agreement with taxonomic classification. Comparisons of the LSU D2 and the ITS2 ribosomal regions from environmental samples and known communities showed similar discriminatory abilities of the two primer sets. Altogether, these findings show that the LR22R–LR3 primer pair has utility for phylogenetic analyses of fungal communities using high-throughput sequencing methods.« less

  18. Advances in high-throughput speed, low-latency communication for embedded instrumentation ( 7th Annual SFAF Meeting, 2012)

    SciTech Connect (OSTI)

    Jordan, Scott

    2012-06-01

    Scott Jordan on "Advances in high-throughput speed, low-latency communication for embedded instrumentation" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  19. High Throughput Computing Impact on Meta Genomics (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    ScienceCinema (OSTI)

    Gore, Brooklin [Morgridge Institute for Research

    2013-01-22

    This presentation includes a brief background on High Throughput Computing, correlating gene transcription factors, optical mapping, genotype to phenotype mapping via QTL analysis, and current work on next gen sequencing.

  20. Advances in high-throughput speed, low-latency communication for embedded instrumentation ( 7th Annual SFAF Meeting, 2012)

    ScienceCinema (OSTI)

    Jordan, Scott [Physik Instrumente

    2013-02-11

    Scott Jordan on "Advances in high-throughput speed, low-latency communication for embedded instrumentation" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  1. High Throughput Plasmid Sequencing with Illumina and CLC Bio (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema (OSTI)

    Athavale, Ajay [Monsanto

    2013-01-25

    Ajay Athavale (Monsanto) presents "High Throughput Plasmid Sequencing with Illumina and CLC Bio" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  2. High Throughput Computing Impact on Meta Genomics (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    SciTech Connect (OSTI)

    Gore, Brooklin [Morgridge Institute for Research] [Morgridge Institute for Research

    2011-10-12

    This presentation includes a brief background on High Throughput Computing, correlating gene transcription factors, optical mapping, genotype to phenotype mapping via QTL analysis, and current work on next gen sequencing.

  3. High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins

    DOE Patents [OSTI]

    Kim, Sung-Hou; Kim, Rosalind; Jancarik, Jamila

    2012-01-31

    An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.

  4. Conceptual design of a high throughput electrorefining of a uranium by using graphite cathode

    SciTech Connect (OSTI)

    Lee, J.H.; Kang, Y.H.; Hwang, S.C.; Park, S.B.; Shim, J.B.; Lee, H.S.; Kim, E.H.; Park, S.W.

    2007-07-01

    Conceptual designing of a high throughput electro-refiner was performed by using basic experimental data and a commercial computational fluid dynamic code, CFX. An electro-refiner concept equipped with a graphite cathode bundle was designed to recover a high purity uranium product continuously without a noble metal contamination. The performance of the process for a decontamination of a noble metal in a uranium product was evaluated as a function of the process parameters such as the rotation speeds of the stirrer and the anode basket. (authors)

  5. Using High Performance Libraries and Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    High Performance Libraries and Tools Using High Performance Libraries and Tools Memkind Library on Edison The memkind library is a user extensible heap manager built on top of jemalloc which enables control of memory characteristics and a partitioning of the heap between kinds of memory (including user defined kinds of memory). This library can be used to simulate the benefit of the high bandwidth memory that will be available on KNL system on the dual socket Edison compute nodes (the two

  6. High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhou, Jizhong; He, Zhili; Yang, Yunfeng; Deng, Ye; Tringe, Susannah G.; Alvarez-Cohen, Lisa

    2015-01-27

    Understanding the structure, functions, activities and dynamics of microbial communities in natural environments is one of the grand challenges of 21st century science. To address this challenge, over the past decade, numerous technologies have been developed for interrogating microbial communities, of which some are amenable to exploratory work (e.g., high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e.g., phylogenetic and functional gene arrays). Here, we provide a critical review and synthesis of the most commonly applied “open-format” and “closed-format” detection technologies. We discuss their characteristics, advantages, and disadvantages within the context of environmental applications andmore » focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference genomes are few. In addition, we discuss crucial issues and considerations associated with applying complementary high-throughput molecular technologies to address important ecological questions.« less

  7. Development of a high-throughput microfluidic integrated microarray for the detection of chimeric bioweapons.

    SciTech Connect (OSTI)

    Sheppod, Timothy; Satterfield, Brent; Hukari, Kyle W.; West, Jason A. A.; Hux, Gary A.

    2006-10-01

    The advancement of DNA cloning has significantly augmented the potential threat of a focused bioweapon assault, such as a terrorist attack. With current DNA cloning techniques, toxin genes from the most dangerous (but environmentally labile) bacterial or viral organism can now be selected and inserted into robust organism to produce an infinite number of deadly chimeric bioweapons. In order to neutralize such a threat, accurate detection of the expressed toxin genes, rather than classification on strain or genealogical decent of these organisms, is critical. The development of a high-throughput microarray approach will enable the detection of unknowns chimeric bioweapons. The development of a high-throughput microarray approach will enable the detection of unknown bioweapons. We have developed a unique microfluidic approach to capture and concentrate these threat genes (mRNA's) upto a 30 fold concentration. These captured oligonucleotides can then be used to synthesize in situ oligonucleotide copies (cDNA probes) of the captured genes. An integrated microfluidic architecture will enable us to control flows of reagents, perform clean-up steps and finally elute nanoliter volumes of synthesized oligonucleotides probes. The integrated approach has enabled a process where chimeric or conventional bioweapons can rapidly be identified based on their toxic function, rather than being restricted to information that may not identify the critical nature of the threat.

  8. Heterogeneous high throughput scientific computing with APM X-Gene and Intel Xeon Phi

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Abdurachmanov, David; Bockelman, Brian; Elmer, Peter; Eulisse, Giulio; Knight, Robert; Muzaffar, Shahzad

    2015-01-01

    Electrical power requirements will be a constraint on the future growth of Distributed High Throughput Computing (DHTC) as used by High Energy Physics. Performance-per-watt is a critical metric for the evaluation of computer architectures for cost- efficient computing. Additionally, future performance growth will come from heterogeneous, many-core, and high computing density platforms with specialized processors. In this paper, we examine the Intel Xeon Phi Many Integrated Cores (MIC) co-processor and Applied Micro X-Gene ARMv8 64-bit low-power server system-on-a-chip (SoC) solutions for scientific computing applications. As a result, we report our experience on software porting, performance and energy efficiency and evaluatemore » the potential for use of such technologies in the context of distributed computing systems such as the Worldwide LHC Computing Grid (WLCG).« less

  9. Optics assembly for high power laser tools

    DOE Patents [OSTI]

    Fraze, Jason D.; Faircloth, Brian O.; Zediker, Mark S.

    2016-06-07

    There is provided a high power laser rotational optical assembly for use with, or in high power laser tools for performing high power laser operations. In particular, the optical assembly finds applications in performing high power laser operations on, and in, remote and difficult to access locations. The optical assembly has rotational seals and bearing configurations to avoid contamination of the laser beam path and optics.

  10. Pneumatic Microvalve-Based Hydrodynamic Sample Injection for High-Throughput, Quantitative Zone Electrophoresis in Capillaries

    SciTech Connect (OSTI)

    Kelly, Ryan T.; Wang, Chenchen; Rausch, Sarah J.; Lee, Cheng S.; Tang, Keqi

    2014-07-01

    A hybrid microchip/capillary CE system was developed to allow unbiased and lossless sample loading and high throughput repeated injections. This new hybrid CE system consists of a polydimethylsiloxane (PDMS) microchip sample injector featuring a pneumatic microvalve that separates a sample introduction channel from a short sample loading channel and a fused silica capillary separation column that connects seamlessly to the sample loading channel. The sample introduction channel is pressurized such that when the pneumatic microvalve opens briefly, a variable-volume sample plug is introduced into the loading channel. A high voltage for CE separation is continuously applied across the loading channel and the fused silica capillary separation column. Analytes are rapidly separated in the fused silica capillary with high resolution. High sensitivity MS detection after CE separation is accomplished via a sheathless CE/ESI-MS interface. The performance evaluation of the complete CE/ESI-MS platform demonstrated that reproducible sample injection with well controlled sample plug volumes could be achieved by using the PDMS microchip injector. The absence of band broadening from microchip to capillary indicated a minimum dead volume at the junction. The capabilities of the new CE/ESI-MS platform in performing high throughput and quantitative sample analyses were demonstrated by the repeated sample injection without interrupting an ongoing separation and a good linear dependence of the total analyte ion abundance on the sample plug volume using a mixture of peptide standards. The separation efficiency of the new platform was also evaluated systematically at different sample injection times, flow rates and CE separation voltages.

  11. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bell, Ronald E.

    2014-07-11

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ≤ 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. The computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection,more » and wavelength calibration.« less

  12. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera

    SciTech Connect (OSTI)

    Bell, Ronald E.

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ? 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  13. Development and operation of a high-throughput accurate-wavelength lens-based spectrometer

    SciTech Connect (OSTI)

    Bell, Ronald E.

    2014-11-15

    A high-throughput spectrometer for the 400820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm{sup ?1} grating is matched with fast f/1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ?0.075 arc sec. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount at the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  14. Development and Operation of High-throughput Accurate-wavelength Lens-based Spectrometer

    SciTech Connect (OSTI)

    Bell, Ronald E

    2014-07-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy < 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  15. Solid optical ring interferometer for high-throughput feedback-free spectral analysis and filtering

    SciTech Connect (OSTI)

    Petrak, B.; Peiris, M.; Muller, A.

    2015-02-15

    We describe a simple and inexpensive optical ring interferometer for use in high-resolution spectral analysis and filtering. It consists of a solid cuboid, reflection-coated on two opposite sides, in which constructive interference occurs for waves in a rhombic trajectory. Due to its monolithic design, the interferometers resonance frequencies are insensitive to environmental disturbances over time. Additional advantages are its simplicity of alignment, high-throughput, and feedback-free operation. If desired, it can be stabilized with a secondary laser without disturbance of the primary signal. We illustrate the use of the interferometer for the measurement of the spectral Mollow triplet from a quantum dot and characterize its long-term stability for filtering applications.

  16. Upgrading a high-throughput spectrometer for high-frequency (&lt;400 kHz) measurements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Upgrading a high-throughput spectrometer for high-frequency ( T. Nishizawa, M. D. Nornberg, D. J. Den Hartog, and D. Craig Citation: Review of Scientific Instruments 87, 11E530 (2016); doi: 10.1063/1.4960073 View online: http://dx.doi.org/10.1063/1.4960073 View Table of Contents: http://scitation.aip.org/content/aip/journal/rsi/87/11?ver=pdfcov Published by the AIP Publishing Articles you may be interested in Observation of 20-400 kHz fluctuations in the U-3M torsatron Phys. Plasmas 23, 022506

  17. Metalloproteomics: High-Throughput Structural and Functional Annotation of Proteins in Structural Genomics

    SciTech Connect (OSTI)

    Shi,W.; Zhan, C.; Lgnatov, A.; Manjasetty, B.; Marinkovic, N.; Sullivan, M.; Huang, R.; Chance, M.; Li, H.; et al.

    2005-01-01

    A high-throughput method for measuring transition metal content based on quantitation of X-ray fluorescence signals was used to analyze 654 proteins selected as targets by the New York Structural GenomiX Research Consortium. Over 10% showed the presence of transition metal atoms in stoichiometric amounts; these totals as well as the abundance distribution are similar to those of the Protein Data Bank. Bioinformatics analysis of the identified metalloproteins in most cases supported the metalloprotein annotation; identification of the conserved metal binding motif was also shown to be useful in verifying structural models of the proteins. Metalloproteomics provides a rapid structural and functional annotation for these sequences and is shown to be {approx}95% accurate in predicting the presence or absence of stoichiometric metal content. The project's goal is to assay at least 1 member from each Pfam family; approximately 500 Pfam families have been characterized with respect to transition metal content so far.

  18. Thicker, more efficient superconducting strip-line detectors for high throughput macromolecules analysis

    SciTech Connect (OSTI)

    Casaburi, A.; Ejrnaes, M.; Cristiano, R.; Zen, N.; Ohkubo, M.; Pagano, S.

    2011-01-10

    Fast detectors with large area are required in time-of-flight mass spectrometers for high throughput analysis of biological molecules. We fabricated and characterized subnanosecond 1x1 mm{sup 2} NbN superconducting strip-line detectors. The influence of the strip-line thickness on the temporal characteristics and efficiency of the detector for the impacts of keV accelerated molecules is investigated. We find that the increase of thickness improves both efficiency and response time. In the thicker sample we achieved a rise time of 380 ps, a fall time of 1.38 ns, and a higher count rate. The physics involved in this behavior is investigated.

  19. High-throughput generation, optimization and analysis of genome-scale metabolic models.

    SciTech Connect (OSTI)

    Henry, C. S.; DeJongh, M.; Best, A. A.; Frybarger, P. M.; Linsay, B.; Stevens, R. L.

    2010-09-01

    Genome-scale metabolic models have proven to be valuable for predicting organism phenotypes from genotypes. Yet efforts to develop new models are failing to keep pace with genome sequencing. To address this problem, we introduce the Model SEED, a web-based resource for high-throughput generation, optimization and analysis of genome-scale metabolic models. The Model SEED integrates existing methods and introduces techniques to automate nearly every step of this process, taking {approx}48 h to reconstruct a metabolic model from an assembled genome sequence. We apply this resource to generate 130 genome-scale metabolic models representing a taxonomically diverse set of bacteria. Twenty-two of the models were validated against available gene essentiality and Biolog data, with the average model accuracy determined to be 66% before optimization and 87% after optimization.

  20. Integrated crystal mounting and alignment system for high-throughput biological crystallography

    DOE Patents [OSTI]

    Nordmeyer, Robert A.; Snell, Gyorgy P.; Cornell, Earl W.; Kolbe, William; Yegian, Derek; Earnest, Thomas N.; Jaklevic, Joseph M.; Cork, Carl W.; Santarsiero, Bernard D.; Stevens, Raymond C.

    2005-07-19

    A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

  1. Integrated crystal mounting and alignment system for high-throughput biological crystallography

    DOE Patents [OSTI]

    Nordmeyer, Robert A.; Snell, Gyorgy P.; Cornell, Earl W.; Kolbe, William F.; Yegian, Derek T.; Earnest, Thomas N.; Jaklevich, Joseph M.; Cork, Carl W.; Santarsiero, Bernard D.; Stevens, Raymond C.

    2007-09-25

    A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

  2. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2015-01-01

    An acoustic high-throughput screening method is described for harvesting protein crystals and combining the protein crystals with chemicals such as a fragment library. Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s{sup −1}) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  3. High-throughput liquid-absorption air-sampling apparatus and methods

    DOE Patents [OSTI]

    Zaromb, Solomon

    2000-01-01

    A portable high-throughput liquid-absorption air sampler [PHTLAAS] has an asymmetric air inlet through which air is drawn upward by a small and light-weight centrifugal fan driven by a direct current motor that can be powered by a battery. The air inlet is so configured as to impart both rotational and downward components of motion to the sampled air near said inlet. The PHTLAAS comprises a glass tube of relatively small size through which air passes at a high rate in a swirling, highly turbulent motion, which facilitates rapid transfer of vapors and particulates to a liquid film covering the inner walls of the tube. The pressure drop through the glass tube is <10 cm of water, usually <5 cm of water. The sampler's collection efficiency is usually >20% for vapors or airborne particulates in the 2-3.mu. range and >50% for particles larger than 4.mu.. In conjunction with various analyzers, the PHTLAAS can serve to monitor a variety of hazardous or illicit airborne substances, such as lead-containing particulates, tritiated water vapor, biological aerosols, or traces of concealed drugs or explosives.

  4. Evaluation of a New Remote Handling Design for High Throughput Annular Centrifugal Contactors

    SciTech Connect (OSTI)

    David H. Meikrantz; Troy G. Garn; Jack D. Law; Lawrence L. Macaluso

    2009-09-01

    Advanced designs of nuclear fuel recycling plants are expected to include more ambitious goals for aqueous based separations including; higher separations efficiency, high-level waste minimization, and a greater focus on continuous processes to minimize cost and footprint. Therefore, Annular Centrifugal Contactors (ACCs) are destined to play a more important role for such future processing schemes. Previous efforts defined and characterized the performance of commercial 5 cm and 12.5 cm single-stage ACCs in a cold environment. The next logical step, the design and evaluation of remote capable pilot scale ACCs in a hot or radioactive environment was reported earlier. This report includes the development of remote designs for ACCs that can process the large throughput rates needed in future nuclear fuel recycling plants. Novel designs were developed for the remote interconnection of contactor units, clean-in-place and drain connections, and a new solids removal collection chamber. A three stage, 12.5 cm diameter rotor module has been constructed and evaluated for operational function and remote handling in highly radioactive environments. This design is scalable to commercial CINC ACC models from V-05 to V-20 with total throughput rates ranging from 20 to 650 liters per minute. The V-05R three stage prototype was manufactured by the commercial vendor for ACCs in the U.S., CINC mfg. It employs three standard V-05 clean-in-place (CIP) units modified for remote service and replacement via new methods of connection for solution inlets, outlets, drain and CIP. Hydraulic testing and functional checks were successfully conducted and then the prototype was evaluated for remote handling and maintenance suitability. Removal and replacement of the center position V-05R ACC unit in the three stage prototype was demonstrated using an overhead rail mounted PaR manipulator. This evaluation confirmed the efficacy of this innovative design for interconnecting and cleaning

  5. A search model for topological insulators with high-throughput robustness descriptors

    SciTech Connect (OSTI)

    Yang, Kesong; Setyawan, Wahyu; Wang, Shidong; Nardelli, Marco B.; Curtarolo, Stephano

    2012-05-13

    Topological insulators (TI) are becoming one of the most studied classes of novel materials because of their great potential for applications ranging from spintronics to quantum computers. To fully integrate TI materials in electronic devices, high-quality epitaxial single-crystalline phases with sufficiently large bulk bandgaps are necessary. Current efforts have relied mostly on costly and time-consuming trial-and-error procedures. Here we show that by defining a reliable and accessible descriptor {cflx X}TI, which represents the topological robustness or feasibility of the candidate, and by searching the quantum materials repository aflowlib.org, we have automatically discovered 28 TIs (some of them already known) in five different symmetry families. These include peculiar ternary halides, Cs{l_brace}Sn,Pb,Ge{r_brace}{l_brace}Cl,Br,I{r_brace}{sub 3}, which could have been hardly anticipated without high-throughput means. Our search model, by relying on the significance of repositories in materials development, opens new avenues for the discovery of more TIs in different and unexplored classes of systems.

  6. FBI Fingerprint Image Capture System High-Speed-Front-End throughput modeling

    SciTech Connect (OSTI)

    Rathke, P.M.

    1993-09-01

    The Federal Bureau of Investigation (FBI) has undertaken a major modernization effort called the Integrated Automated Fingerprint Identification System (IAFISS). This system will provide centralized identification services using automated fingerprint, subject descriptor, mugshot, and document processing. A high-speed Fingerprint Image Capture System (FICS) is under development as part of the IAFIS program. The FICS will capture digital and microfilm images of FBI fingerprint cards for input into a central database. One FICS design supports two front-end scanning subsystems, known as the High-Speed-Front-End (HSFE) and Low-Speed-Front-End, to supply image data to a common data processing subsystem. The production rate of the HSFE is critical to meeting the FBI`s fingerprint card processing schedule. A model of the HSFE has been developed to help identify the issues driving the production rate, assist in the development of component specifications, and guide the evolution of an operations plan. A description of the model development is given, the assumptions are presented, and some HSFE throughput analysis is performed.

  7. An ultra-compact, high-throughput molecular beam epitaxy growth system

    SciTech Connect (OSTI)

    Baker, A. A.; Hesjedal, T.; Braun, W. E-mail: fischer@createc.de; Rembold, S.; Fischer, A. E-mail: fischer@createc.de; Gassler, G.

    2015-04-15

    We present a miniaturized molecular beam epitaxy (miniMBE) system with an outer diameter of 206 mm, optimized for flexible and high-throughput operation. The three-chamber system, used here for oxide growth, consists of a sample loading chamber, a storage chamber, and a growth chamber. The growth chamber is equipped with eight identical effusion cell ports with linear shutters, one larger port for either a multi-pocket electron beam evaporator or an oxygen plasma source, an integrated cryoshroud, retractable beam-flux monitor or quartz-crystal microbalance, reflection high energy electron diffraction, substrate manipulator, main shutter, and quadrupole mass spectrometer. The system can be combined with ultrahigh vacuum (UHV) end stations on synchrotron and neutron beamlines, or equivalently with other complex surface analysis systems, including low-temperature scanning probe microscopy systems. Substrate handling is compatible with most UHV surface characterization systems, as the miniMBE can accommodate standard surface science sample holders. We introduce the design of the system, and its specific capabilities and operational parameters, and we demonstrate the epitaxial thin film growth of magnetoelectric Cr{sub 2}O{sub 3} on c-plane sapphire and ferrimagnetic Fe{sub 3}O{sub 4} on MgO (001)

  8. Metal Organic Framework Research: High Throughput Discovery of Robust Metal Organic Framework for CO2 Capture

    SciTech Connect (OSTI)

    2010-08-01

    IMPACCT Project: LBNL is developing a method for identifying the best metal organic frameworks for use in capturing CO2 from the flue gas of coal-fired power plants. Metal organic frameworks are porous, crystalline compounds that, based on their chemical structure, vary considerably in terms of their capacity to grab hold of passing CO2 molecules and their ability to withstand the harsh conditions found in the gas exhaust of coal-fired power plants. Owing primarily to their high tunability, metal organic frameworks can have an incredibly wide range of different chemical and physical properties, so identifying the best to use for CO2 capture and storage can be a difficult task. LBNL uses high-throughput instrumentation to analyze nearly 100 materials at a time, screening them for the characteristics that optimize their ability to selectively adsorb CO2 from coal exhaust. Their work will identify the most promising frameworks and accelerate their large-scale commercial development to benefit further research into reducing the cost of CO2 capture and storage.

  9. High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

    SciTech Connect (OSTI)

    Musah, Rabi A.; Espinoza, Edgard O.; Cody, Robert B.; Lesiak, Ashton D.; Christensen, Earl D.; Moore, Hannah E.; Maleknia, Simin; Drijhout, Falko P.

    2015-07-09

    A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. Moreover, a range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. In this paper, we illustrate how the method can be used to: (1) distinguish between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required.

  10. High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Musah, Rabi A.; Espinoza, Edgard O.; Cody, Robert B.; Lesiak, Ashton D.; Christensen, Earl D.; Moore, Hannah E.; Maleknia, Simin; Drijhout, Falko P.

    2015-07-09

    A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. Moreover, a range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. In this paper, we illustrate how the method can be used to: (1) distinguishmore » between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required.« less

  11. Bonus Organisms in High-Throughput Eukaryotic Whole-Genome Shorgun Assembly

    SciTech Connect (OSTI)

    Pangilinan, Jasmyn; Shapiro, Harris; Tu, Hank; Platt, Darren

    2006-02-06

    The DOE Joint Genome Institute has sequenced over 50 eukaryotic genomes, ranging in size from 15 MB to 1.6 GB, over a wide range of organism types. In the course of doing so, it has become clear that a substantial fraction of these data sets contains bonus organisms, usually prokaryotes, in addition to the desired genome. While some of these additional organisms are extraneous contamination, they are sometimes symbionts, and so can be of biological interest. Therefore, it is desirable to assemble the bonus organisms along with the main genome. This transforms the problem into one of metagenomic assembly, which is considerably more challenging than traditional whole-genome shotgun (WGS) assembly. The different organisms will usually be present at different sequence depths, which is difficult to handle in most WGS assemblers. In addition, with multiple distinct genomes present, chimerism can produce cross-organism combinations. Finally, there is no guarantee that only a single bonus organism will be present. For example, one JGI project contained at least two different prokaryotic contaminants, plus a 145 KB plasmid of unknown origin. We have developed techniques to routinely identify and handle such bonus organisms in a high-throughput sequencing environment. Approaches include screening and partitioning the unassembled data, and iterative subassemblies. These methods are applicable not only to bonus organisms, but also to desired components such as organelles. These procedures have the additional benefit of identifying, and allowing for the removal of, cloning artifacts such as E.coli and spurious vector inclusions.

  12. High-throughput Characterization of Porous Materials Using Graphics Processing Units

    SciTech Connect (OSTI)

    Kim, Jihan; Martin, Richard L.; Ruebel, Oliver; Haranczyk, Maciej; Smit, Berend

    2012-03-19

    We have developed a high-throughput graphics processing units (GPU) code that can characterize a large database of crystalline porous materials. In our algorithm, the GPU is utilized to accelerate energy grid calculations where the grid values represent interactions (i.e., Lennard-Jones + Coulomb potentials) between gas molecules (i.e., CH$_{4}$ and CO$_{2}$) and material's framework atoms. Using a parallel flood fill CPU algorithm, inaccessible regions inside the framework structures are identified and blocked based on their energy profiles. Finally, we compute the Henry coefficients and heats of adsorption through statistical Widom insertion Monte Carlo moves in the domain restricted to the accessible space. The code offers significant speedup over a single core CPU code and allows us to characterize a set of porous materials at least an order of magnitude larger than ones considered in earlier studies. For structures selected from such a prescreening algorithm, full adsorption isotherms can be calculated by conducting multiple grand canonical Monte Carlo simulations concurrently within the GPU.

  13. Gasification of refuse derived fuel in the Battelle high throughput gasification system

    SciTech Connect (OSTI)

    Paisley, M.A.; Creamer, K.S.; Tweksbury, T.L.; Taylor, D.R. )

    1989-07-01

    This report presents the results of an experimental program to demonstrate the suitability of the Battelle High Throughput Gasification Process to non-wood biomass fuels. An extensive data base on wood gasification was generated during a multi-year experimental program. This data base and subsequent design and economic analysis activities led to the discussion to study the gasification character of other fuels. The specific fuel studied was refuse derived fuel (RDF) which is a prepared municipal solid waste (MSW). The use of RDF, while providing a valuable fuel, can also provide a solution to MSW disposal problems. Gasification of MSW provides advantages over land fill or mass burn technology since a more usable form of energy, medium Btu gas, is produced. Land filling of wastes produces no usable products and mass burning while greatly reducing the volume of wastes for disposal can produce only steam. This steam must be used on site or very nearby this limiting the potential locations for mass burn facilities. Such a gas, if produced from currently available supplies of MSW, can contribute 2 quads to the US energy supply. 3 refs., 12 figs., 7 tabs.

  14. Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates

    SciTech Connect (OSTI)

    Hamilton-Brehm, Scott; Vishnivetskaya, Tatiana A; Allman, Steve L; Mielenz, Jonathan R; Elkins, James G

    2012-01-01

    Flow cytometry (FCM) techniques have been developed for sorting mesophilic organisms, but the difficulty increases if the target microbes are thermophilic anaerobes. We demonstrate a reliable, high-throughput method of screening thermophilic anaerobic organisms using FCM and 96-well plates for growth on biomass-relevant substrates. The method was tested using the cellulolytic thermophiles Clostridium ther- mocellum (Topt = 55 C), Caldicellulosiruptor obsidiansis (Topt = 78 C) and the fermentative hyperthermo- philes, Pyrococcus furiosus (Topt = 100 C) and Thermotoga maritima (Topt = 80 C). Multi-well plates were incubated at various temperatures for approximately 72 120 h and then tested for growth. Positive growth resulting from single cells sorted into individual wells containing an anaerobic medium was verified by OD600. Depending on the growth substrate, up to 80 % of the wells contained viable cultures, which could be transferred to fresh media. This method was used to isolate thermophilic microbes from Rabbit Creek, Yellowstone National Park (YNP), Wyoming. Substrates for enrichment cultures including crystalline cellulose (Avicel), xylan (from Birchwood), pretreated switchgrass and Populus were used to cultivate organisms that may be of interest to lignocellulosic biofuel production.

  15. Integrated Controlling System and Unified Database for High Throughput Protein Crystallography Experiments

    SciTech Connect (OSTI)

    Gaponov, Yu.A.; Igarashi, N.; Hiraki, M.; Sasajima, K.; Matsugaki, N.; Suzuki, M.; Kosuge, T.; Wakatsuki, S.

    2004-05-12

    An integrated controlling system and a unified database for high throughput protein crystallography experiments have been developed. Main features of protein crystallography experiments (purification, crystallization, crystal harvesting, data collection, data processing) were integrated into the software under development. All information necessary to perform protein crystallography experiments is stored (except raw X-ray data that are stored in a central data server) in a MySQL relational database. The database contains four mutually linked hierarchical trees describing protein crystals, data collection of protein crystal and experimental data processing. A database editor was designed and developed. The editor supports basic database functions to view, create, modify and delete user records in the database. Two search engines were realized: direct search of necessary information in the database and object oriented search. The system is based on TCP/IP secure UNIX sockets with four predefined sending and receiving behaviors, which support communications between all connected servers and clients with remote control functions (creating and modifying data for experimental conditions, data acquisition, viewing experimental data, and performing data processing). Two secure login schemes were designed and developed: a direct method (using the developed Linux clients with secure connection) and an indirect method (using the secure SSL connection using secure X11 support from any operating system with X-terminal and SSH support). A part of the system has been implemented on a new MAD beam line, NW12, at the Photon Factory Advanced Ring for general user experiments.

  16. High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Teplitsky, Ella; Joshi, Karan; Ericson, Daniel L.; Scalia, Alexander; Mullen, Jeffrey D.; Sweet, Robert M.; Soares, Alexei S.

    2015-07-01

    We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using thismore » system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. Moreover, a fragment mini-library was screened to observe two known lysozyme We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. A fragment mini-library was screened to observe two known

  17. High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density

    SciTech Connect (OSTI)

    Teplitsky, Ella; Joshi, Karan; Ericson, Daniel L.; Scalia, Alexander; Mullen, Jeffrey D.; Sweet, Robert M.; Soares, Alexei S.

    2015-07-01

    We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. Moreover, a fragment mini-library was screened to observe two known lysozyme We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5 nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate™ for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5 nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. A fragment mini-library was screened to observe two known lysozyme

  18. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    SciTech Connect (OSTI)

    Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

    2009-01-05

    We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

  19. New Composite Membranes for High Throughput Solid-Liquid Separations at the Savannah River Site

    SciTech Connect (OSTI)

    Bhave, Ramesh R

    2012-01-01

    New Composite Membranes for High Throughput Solid-Liquid Separations at the Savannah River Site R. Bhave (Oak Ridge National Laboratory. Oak Ridge, TN) and M. R. Poirier* (Savannah River National Laboratory, Aiken SC) Solid-liquid separation is the limiting step for many waste treatment processes at the Savannah River Site. SRNL researchers have identified the rotary microfilter as a technology to improve the rate of solid-liquid separation processes. SRNL is currently developing the rotary microfilter for radioactive service and plans to deploy the technology as part of the small column ion exchange process. The rotary microfilter can utilize any filter media that is available as a flat sheet. The current baseline membrane is a 0.5 micron (nominal) porous metal filter (Pall PMM050). Previous testing with tubular filters showed that filters composed of a ceramic membrane on top of a stainless steel support produce higher flux than filters composed only of porous metal. The authors are working to develop flat sheet filter media composed of a ceramic membrane and/or ceramic-metal composite on top of a porous stainless steel support that can be used with the rotary microfilter to substantially increase filter flux resulting in a more compact, energy efficient and cost-effective high level radioactive waste treatment system. Composite membranes with precisely controlled pore size distribution were fabricated on porous metal supports. High quality uniform porous metal (316SS) supports were fabricated to achieve high water permeability. Separative layers of several different materials such as ultrafine metal particles and ceramic oxides were used to fabricate composite membranes. The fabrication process involved several high temperature heat treatments followed by characterization of gas and liquid permeability measurements and membrane integrity analysis. The fabricated composite membrane samples were evaluated in a static test cell manufactured by SpinTek. The

  20. A reactor for high-throughput high-pressure nuclear magnetic resonance spectroscopy

    SciTech Connect (OSTI)

    Beach, N. J.; Knapp, S. M. M.; Landis, C. R.

    2015-10-15

    The design of a reactor for operando nuclear magnetic resonance (NMR) monitoring of high-pressure gas-liquid reactions is described. The Wisconsin High Pressure NMR Reactor (WiHP-NMRR) design comprises four modules: a sapphire NMR tube with titanium tube holder rated for pressures as high as 1000 psig (68 atm) and temperatures ranging from −90 to 90 °C, a gas circulation system that maintains equilibrium concentrations of dissolved gases during gas-consuming or gas-releasing reactions, a liquid injection apparatus that is capable of adding measured amounts of solutions to the reactor under high pressure conditions, and a rapid wash system that enables the reactor to be cleaned without removal from the NMR instrument. The WiHP-NMRR is compatible with commercial 10 mm NMR probes. Reactions performed in the WiHP-NMRR yield high quality, information-rich, and multinuclear NMR data over the entire reaction time course with rapid experimental turnaround.

  1. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2014-06-01

    Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  2. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; et al

    2014-06-01

    Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from themore » inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.« less

  3. WE-E-BRE-07: High-Throughput Mapping of Proton Biologic Effect

    SciTech Connect (OSTI)

    Bronk, L; Guan, F; Kerr, M; Dinh, J; Titt, U; Mirkovic, D; Lin, S; Mohan, R; Grosshans, D

    2014-06-15

    Purpose: To systematically relate the relative biological effectives (RBE) of proton therapy to beam linear energy transfer (LET) and dose. Methods: Using a custom irradiation apparatus previously characterized by our group, H460 NSCLCs were irradiated using a clinical 80MeV spot scanning proton beam. Utilizing this system allowed for high-throughput clonogenic assays performed in 96-well tissue culture plates as opposed to the traditional 6-well technique. Each column in the 96-well plate received a set LET-dose combination. By altering the total number of dose repaintings, numerous dose-LET configurations were examined to effectively generate surviving fraction (SF) data over the entire Bragg peak. The clonogenic assay was performed post-irradiation using an INCell Analyzer for colony quantification. SF data were fit to the linear-quadratic model for analysis. Results: Irradiation with increasing LETs resulted in decreased cell survival largely independent of dose. A significant correlation between LET and SF was identified by two-way ANOVA and the extra sum-of-squares F test. This trend was obscured at the lower LET values in the plateau region of the Bragg peak; however, it was clear for LET values at and beyond the Bragg peak. Data fits revealed the SF at a dose of 2Gy (SF2) to be 0.48 for the lowest tested LET (1.55keV/um), 0.47 at the end of the plateau region (4.74keV/um) and 0.33 for protons at the Bragg peak (10.35keV/um). Beyond the Bragg peak we measured SF2s of 0.16 for 15.01keV/um, 0.02 for 16.79keV/um, and 0.004 for 18.06keV/um. Conclusion: We have shown that our methodology enables high-content automated screening for proton irradiations over a range of LETs. The observed decrease in cellular SF in high LET regions confirms an increased RBE of the radiation and suggests further evaluation of proton RBE values is necessary to optimize clinical outcomes. Rosalie B. Hite Graduate Fellowship in Cancer Research, NIH Program Project Grant P01CA021239.

  4. Full Reviews: High-temperature Tools and Drilling

    Broader source: Energy.gov [DOE]

    Below are the project presentations and respective peer reviewer comments for High-temperature Tools and Drilling.

  5. Scalable Computational Methods for the Analysis of High-Throughput Biological Data

    SciTech Connect (OSTI)

    Langston, Michael A

    2012-09-06

    This primary focus of this research project is elucidating genetic regulatory mechanisms that control an organism?¢????s responses to low-dose ionizing radiation. Although low doses (at most ten centigrays) are not lethal to humans, they elicit a highly complex physiological response, with the ultimate outcome in terms of risk to human health unknown. The tools of molecular biology and computational science will be harnessed to study coordinated changes in gene expression that orchestrate the mechanisms a cell uses to manage the radiation stimulus. High performance implementations of novel algorithms that exploit the principles of fixed-parameter tractability will be used to extract gene sets suggestive of co-regulation. Genomic mining will be performed to scrutinize, winnow and highlight the most promising gene sets for more detailed investigation. The overall goal is to increase our understanding of the health risks associated with exposures to low levels of radiation.

  6. New Challenges of the Computation of Multiple Sequence Alignments in the High-Throughput Era (2010 JGI/ANL HPC Workshop)

    ScienceCinema (OSTI)

    Notredame, Cedric [Centre for Genomic Regulation

    2011-06-08

    Cedric Notredame from the Centre for Genomic Regulation gives a presentation on "New Challenges of the Computation of Multiple Sequence Alignments in the High-Throughput Era" at the JGI/Argonne HPC Workshop on January 26, 2010.

  7. Assessment of advanced coal-gasification processes. [AVCO high throughput gasification in process; Bell High Mass Flux process; CS-R process; and Exxon Gasification process

    SciTech Connect (OSTI)

    McCarthy, J.; Ferrall, J.; Charng, T.; Houseman, J.

    1981-06-01

    This report represents a technical assessment of the following advanced coal gasification processes: AVCO High Throughput Gasification (HTG) Process, Bell Single - Stage High Mass Flux (HMF) Process, Cities Service/Rockwell (CS/R) Hydrogasification Process, and the Exxon Catalytic Coal Gasification (CCG) Process. Each process is evaluated for its potential to produce SNG from a bituminous coal. In addition to identifying the new technology these processes represent, key similarities/differences, strengths/weaknesses, and potential improvements to each process are identified. The AVCO HTG and the Bell HMF gasifiers share similarities with respect to: short residence time (SRT), high throughput rate, slagging and syngas as the initial raw product gas. The CS/R Hydrogasifier is also SRT but is non-slagging and produces a raw gas high in methane content. The Exxon CCG gasifier is a long residence time, catalytic fluidbed reactor producing all of the raw product methane in the gasifier.

  8. High-throughput characterization of stresses in thin film materials libraries using Si cantilever array wafers and digital holographic microscopy

    SciTech Connect (OSTI)

    Lai, Y. W.; Ludwig, A.; Hamann, S.; Ehmann, M.

    2011-06-15

    We report the development of an advanced high-throughput stress characterization method for thin film materials libraries sputter-deposited on micro-machined cantilever arrays consisting of around 1500 cantilevers on 4-inch silicon-on-insulator wafers. A low-cost custom-designed digital holographic microscope (DHM) is employed to simultaneously monitor the thin film thickness, the surface topography and the curvature of each of the cantilevers before and after deposition. The variation in stress state across the thin film materials library is then calculated by Stoney's equation based on the obtained radii of curvature of the cantilevers and film thicknesses. DHM with nanometer-scale out-of-plane resolution allows stress measurements in a wide range, at least from several MPa to several GPa. By using an automatic x-y translation stage, the local stresses within a 4-inch materials library are mapped with high accuracy within 10 min. The speed of measurement is greatly improved compared with the prior laser scanning approach that needs more than an hour of measuring time. A high-throughput stress measurement of an as-deposited Fe-Pd-W materials library was evaluated for demonstration. The fast characterization method is expected to accelerate the development of (functional) thin films, e.g., (magnetic) shape memory materials, whose functionality is greatly stress dependent.

  9. Feasibility and Design Studies for a High Temperature Downhole Tool

    Broader source: Energy.gov [DOE]

    Project objective: Perform feasibility and design studies for a high temperature downhole tool; which uses nuclear techniques for characterization purposes; using measurements and modeling/simulation.

  10. High-Temperature Downhole Tools | Open Energy Information

    Open Energy Info (EERE)

    and Analysis of Geothermal Technologies Albuquerque, NM 941,000 941,000 Feasibility and Design for a High-Temperature Downhole Tool Tennessee Oak Ridge National...

  11. High-throughput, dual probe biological assays based on single molecule detection

    DOE Patents [OSTI]

    Hollars, Christopher W.; Huser, Thomas R.; Lane, Stephen M.; Balhorn, Rodney L.; Bakajin, Olgica; Darrow, Christopher; Satcher, Jr., Joe H.

    2006-07-11

    A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 .mu.m.times.100 .mu.m.times.100 .mu.m. A device using these methods would have 10.sup.3 10.sup.4 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 .mu.m.sup.3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (.about.10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

  12. Risk-based high-throughput chemical screening and prioritization using exposure models and in vitro bioactivity assays

    SciTech Connect (OSTI)

    Shin, Hyeong -Moo; Ernstoff, Alexi; Arnot, Jon A.; Wetmore, Barbara A.; Csiszar, Susan A.; Fantke, Peter; Zhang, Xianming; McKone, Thomas E.; Jolliet, Olivier; Bennett, Deborah H.

    2015-05-01

    We present a risk-based high-throughput screening (HTS) method to identify chemicals for potential health concerns or for which additional information is needed. The method is applied to 180 organic chemicals as a case study. We first obtain information on how the chemical is used and identify relevant use scenarios (e.g., dermal application, indoor emissions). For each chemical and use scenario, exposure models are then used to calculate a chemical intake fraction, or a product intake fraction, accounting for chemical properties and the exposed population. We then combine these intake fractions with use scenario-specific estimates of chemical quantity to calculate daily intake rates (iR; mg/kg/day). These intake rates are compared to oral equivalent doses (OED; mg/kg/day), calculated from a suite of ToxCast in vitro bioactivity assays using in vitro-to-in vivo extrapolation and reverse dosimetry. Bioactivity quotients (BQs) are calculated as iR/OED to obtain estimates of potential impact associated with each relevant use scenario. Of the 180 chemicals considered, 38 had maximum iRs exceeding minimum OEDs (i.e., BQs > 1). For most of these compounds, exposures are associated with direct intake, food/oral contact, or dermal exposure. The method provides high-throughput estimates of exposure and important input for decision makers to identify chemicals of concern for further evaluation with additional information or more refined models.

  13. Risk-based high-throughput chemical screening and prioritization using exposure models and in vitro bioactivity assays

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shin, Hyeong -Moo; Ernstoff, Alexi; Arnot, Jon A.; Wetmore, Barbara A.; Csiszar, Susan A.; Fantke, Peter; Zhang, Xianming; McKone, Thomas E.; Jolliet, Olivier; Bennett, Deborah H.

    2015-05-01

    We present a risk-based high-throughput screening (HTS) method to identify chemicals for potential health concerns or for which additional information is needed. The method is applied to 180 organic chemicals as a case study. We first obtain information on how the chemical is used and identify relevant use scenarios (e.g., dermal application, indoor emissions). For each chemical and use scenario, exposure models are then used to calculate a chemical intake fraction, or a product intake fraction, accounting for chemical properties and the exposed population. We then combine these intake fractions with use scenario-specific estimates of chemical quantity to calculate dailymore » intake rates (iR; mg/kg/day). These intake rates are compared to oral equivalent doses (OED; mg/kg/day), calculated from a suite of ToxCast in vitro bioactivity assays using in vitro-to-in vivo extrapolation and reverse dosimetry. Bioactivity quotients (BQs) are calculated as iR/OED to obtain estimates of potential impact associated with each relevant use scenario. Of the 180 chemicals considered, 38 had maximum iRs exceeding minimum OEDs (i.e., BQs > 1). For most of these compounds, exposures are associated with direct intake, food/oral contact, or dermal exposure. The method provides high-throughput estimates of exposure and important input for decision makers to identify chemicals of concern for further evaluation with additional information or more refined models.« less

  14. RECENT PROCESS AND EQUIPMENT IMPROVEMENTS TO INCREASE HIGH LEVEL WASTE THROUGHPUT AT THE DEFENSE WASTE PROCESSING FACILITY

    SciTech Connect (OSTI)

    Odriscoll, R; Allan Barnes, A; Jim Coleman, J; Timothy Glover, T; Robert Hopkins, R; Dan Iverson, D; Jeff Leita, J

    2008-01-15

    The Savannah River Site's (SRS) Defense Waste Processing Facility (DWPF) began stabilizing high level waste (HLW) in a glass matrix in 1996. Over the past few years, there have been several process and equipment improvements at the DWPF to increase the rate at which the high level waste can be stabilized. These improvements have either directly increased waste processing rates or have desensitized the process to upsets, thereby minimizing downtime and increasing production. Improvements due to optimization of waste throughput with increased HLW loading of the glass resulted in a 6% waste throughput increase based upon operational efficiencies. Improvements in canister production include the pour spout heated bellows liner (5%), glass surge (siphon) protection software (2%), melter feed pump software logic change to prevent spurious interlocks of the feed pump with subsequent dilution of feed stock (2%) and optimization of the steam atomized scrubber (SAS) operation to minimize downtime (3%) for a total increase in canister production of 12%. A number of process recovery efforts have allowed continued operation. These include the off gas system pluggage and restoration, slurry mix evaporator (SME) tank repair and replacement, remote cleaning of melter top head center nozzle, remote melter internal inspection, SAS pump J-Tube recovery, inadvertent pour scenario resolutions, dome heater transformer bus bar cooling water leak repair and new Infra-red camera for determination of glass height in the canister are discussed.

  15. High power laser perforating tools and systems

    DOE Patents [OSTI]

    Zediker, Mark S; Rinzler, Charles C; Faircloth, Brian O; Koblick, Yeshaya; Moxley, Joel F

    2014-04-22

    ystems devices and methods for the transmission of 1 kW or more of laser energy deep into the earth and for the suppression of associated nonlinear phenomena. Systems, devices and methods for the laser perforation of a borehole in the earth. These systems can deliver high power laser energy down a deep borehole, while maintaining the high power to perforate such boreholes.

  16. High throughput exploration of process-property linkages in Al-6061 using instrumented spherical microindentation and microstructurally graded samples

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Weaver, Jordan S.; Khosravani, Ali; Castillo, Andrew; Kalidindi, Surya R.

    2016-06-14

    Recent spherical nanoindentation protocols have proven robust at capturing the local elastic-plastic response of polycrystalline metal samples at length scales much smaller than the grain size. In this work, we extend these protocols to length scales that include multiple grains to recover microindentation stress-strain curves. These new protocols are first established in this paper and then demonstrated for Al-6061 by comparing the measured indentation stress-strain curves with the corresponding measurements from uniaxial tension tests. More specifically, the scaling factors between the uniaxial yield strength and the indentation yield strength was determined to be about 1.9, which is significantly lower thanmore » the value of 2.8 used commonly in literature. Furthermore, the reasons for this difference are discussed. Second, the benefits of these new protocols in facilitating high throughput exploration of process-property relationships are demonstrated through a simple case study.« less

  17. Industrial co-generation through use of a medium BTU gas from biomass produced in a high throughput reactor

    SciTech Connect (OSTI)

    Feldmann, H.F.; Ball, D.A.; Paisley, M.A.

    1983-01-01

    A high-throughput gasification system has been developed for the steam gasification of woody biomass to produce a fuel gas with a heating value of 475 to 500 Btu/SCF without using oxygen. Recent developments have focused on the use of bark and sawdust as feedstocks in addition to wood chips and the testing of a new reactor concept, the so-called controlled turbulent zone (CTZ) reactor to increase gas production per unit of wood fed. Operating data from the original gasification system and the CTZ system are used to examine the preliminary economics of biomass gasification/gas turbine cogeneration systems. In addition, a ''generic'' pressurized oxygen-blown gasification system is evaluated. The economics of these gasification systems are compared with a conventional wood boiler/steam turbine cogeneration system.

  18. Determination of gene expression patterns using high-throughput RNA in situ hybridizaion to whole-mount Drosophila embryos

    SciTech Connect (OSTI)

    Weiszmann, R.; Hammonds, A.S.; Celniker, S.E.

    2009-04-09

    We describe a high-throughput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in a 96-well format. cDNA or genomic DNA templates are amplified by PCR and then digoxigenin-labeled ribonucleotides are incorporated into antisense RNA probes by in vitro transcription. The quality of each probe is evaluated before ISH using a RNA probe quantification (dot blot) assay. RNA probes are hybridized to fixed, mixed-staged Drosophila embryos in 96-well plates. The resulting stained embryos can be examined and photographed immediately or stored at 4oC for later analysis. Starting with fixed, staged embryos, the protocol takes 6 d from probe template production through hybridization. Preparation of fixed embryos requires a minimum of 2 weeks to collect embryos representing all stages. The method has been used to determine the expression patterns of over 6,000 genes throughout embryogenesis.

  19. Understanding the stable boron clusters: A bond model and first-principles calculations based on high-throughput screening

    SciTech Connect (OSTI)

    Xu, Shao-Gang; Liao, Ji-Hai; Zhao, Yu-Jun; Yang, Xiao-Bao

    2015-06-07

    The unique electronic property induced diversified structure of boron (B) cluster has attracted much interest from experimentalists and theorists. B{sub 30–40} were reported to be planar fragments of triangular lattice with proper concentrations of vacancies recently. Here, we have performed high-throughput screening for possible B clusters through the first-principles calculations, including various shapes and distributions of vacancies. As a result, we have determined the structures of B{sub n} clusters with n = 30–51 and found a stable planar cluster of B{sub 49} with a double-hexagon vacancy. Considering the 8-electron rule and the electron delocalization, a concise model for the distribution of the 2c–2e and 3c–2e bonds has been proposed to explain the stability of B planar clusters, as well as the reported B cages.

  20. High-throughput identification of off-targets for the mechanistic study of severe adverse drug reactions induced by analgesics

    SciTech Connect (OSTI)

    Pan, Jian-Bo; Ji, Nan; Pan, Wen; Hong, Ru; Wang, Hao; Ji, Zhi-Liang

    2014-01-01

    Drugs may induce adverse drug reactions (ADRs) when they unexpectedly bind to proteins other than their therapeutic targets. Identification of these undesired protein binding partners, called off-targets, can facilitate toxicity assessment in the early stages of drug development. In this study, a computational framework was introduced for the exploration of idiosyncratic mechanisms underlying analgesic-induced severe adverse drug reactions (SADRs). The putative analgesic-target interactions were predicted by performing reverse docking of analgesics or their active metabolites against human/mammal protein structures in a high-throughput manner. Subsequently, bioinformatics analyses were undertaken to identify ADR-associated proteins (ADRAPs) and pathways. Using the pathways and ADRAPs that this analysis identified, the mechanisms of SADRs such as cardiac disorders were explored. For instance, 53 putative ADRAPs and 24 pathways were linked with cardiac disorders, of which 10 ADRAPs were confirmed by previous experiments. Moreover, it was inferred that pathways such as base excision repair, glycolysis/glyconeogenesis, ErbB signaling, calcium signaling, and phosphatidyl inositol signaling likely play pivotal roles in drug-induced cardiac disorders. In conclusion, our framework offers an opportunity to globally understand SADRs at the molecular level, which has been difficult to realize through experiments. It also provides some valuable clues for drug repurposing. - Highlights: A novel computational framework was developed for mechanistic study of SADRs. Off-targets of drugs were identified in large scale and in a high-throughput manner. SADRs like cardiac disorders were systematically explored in molecular networks. A number of ADR-associated proteins were identified.

  1. The very high cycle fatigue behavior of tool steel materials...

    Office of Scientific and Technical Information (OSTI)

    Title: The very high cycle fatigue behavior of tool steel materials Authors: Shyam, Amit 1 ; Blau, Peter Julian 1 ; Jordan, Tyson L 1 ; Yang, Nan 2 ; Pollard, Michael J 3 ...

  2. Operational Simulation Tools and Long Term Strategic Planning for High

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Penetrations of PV in the Southeastern U.S. | Department of Energy Operational Simulation Tools and Long Term Strategic Planning for High Penetrations of PV in the Southeastern U.S. Operational Simulation Tools and Long Term Strategic Planning for High Penetrations of PV in the Southeastern U.S. EPRI logo.jpg In collaboration with the Tennessee Valley Authority Southern Company, the Sacramento Municipal Utility District, the California Independent System Operator, and other partners,

  3. High throughput lessons from the LHC experience.Johnston.TNC2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... the data management systems, data movement systems, ... "coherent optical" technology providing 100 Gbs ... II", The International Conference on Computing in High ...

  4. High-Throughput Methodology for Discovery of Metal-Organic Frameworks...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Methodology for Discovery of Metal-Organic Frameworks with a High Hydrogen Binding Enthalpy Steven S. Kaye, Satoshi Horike, and Jeffrey R. Long Department of Chemistry, University ...

  5. Development and operation of a high-throughput accurate-wavelength...

    Office of Scientific and Technical Information (OSTI)

    imaging. A precision optical encoder measures the grating angle with an accuracy 0.075 arc sec. A high quantum efficiency low-etaloning CCD detector allows operation at longer...

  6. Air-stable ink for scalable, high-throughput layer deposition

    DOE Patents [OSTI]

    Weil, Benjamin D; Connor, Stephen T; Cui, Yi

    2014-02-11

    A method for producing and depositing air-stable, easily decomposable, vulcanized ink on any of a wide range of substrates is disclosed. The ink enables high-volume production of optoelectronic and/or electronic devices using scalable production methods, such as roll-to-roll transfer, fast rolling processes, and the like.

  7. High-Throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

    SciTech Connect (OSTI)

    Decker, Stephen R.; Sykes, Robert W.; Turner, Geoffrey B.; Lupoi, Jason S.; Doepkke, Crissa; Tucker, Melvin P.; Schuster, Logan A.; Mazza, Kimberly; Himmel, Michael E.; Davis, Mark F.; Gjersing, Erica

    2015-09-15

    The conversion of lignocellulosic biomass to fuels, chemicals, and other commodities has been explored as one possible pathway toward reductions in the use of non-renewable energy sources. In order to identify which plants, out of a diverse pool, have the desired chemical traits for downstream applications, attributes, such as cellulose and lignin content, or monomeric sugar release following an enzymatic saccharification, must be compared. The experimental and data analysis protocols of the standard methods of analysis can be time-consuming, thereby limiting the number of samples that can be measured. High-throughput (HTP) methods alleviate the shortcomings of the standard methods, and permit the rapid screening of available samples to isolate those possessing the desired traits. This study illustrates the HTP sugar release and pyrolysis-molecular beam mass spectrometry pipelines employed at the National Renewable Energy Lab. These pipelines have enabled the efficient assessment of thousands of plants while decreasing experimental time and costs through reductions in labor and consumables.

  8. Evaluation of High Throughput Screening Methods in Picking up Differences between Cultivars of Lignocellulosic Biomass for Ethanol Production

    SciTech Connect (OSTI)

    Lindedam, Jane; Bruun, Sander; Jorgensen, Henning; Decker, Stephen R.; Turner, Geoffrey B.; DeMartini, Jaclyn D.; Wyman, Charles E.; Felby, Claus

    2014-07-01

    Here, we present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. We found the best correlation of glucose yields between the Riverside and NREL systems (R2 = 0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2 = 0.4269). The three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.

  9. Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Geng, Tao; Bredeweg, Erin L.; Szymanski, Craig J.; Liu, Bingwen; Baker, Scott E.; Orr, Galya; Evans, James E.; Kelly, Ryan T.

    2015-11-04

    Here, interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of science applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, this microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level, and quantitative dynamics of gene expression withmore » single hyphal compartment resolution in response to carbon source starvation and exchange experiments. Although the microfluidic device is demonstrated on filamentous fungi, our technology is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth with applications ranging from bioenergy production to human health.« less

  10. Conversion of forest residues to a methane-rich gas in a high-throughput gasifier

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.; Appelbaum, H.R.; Taylor, D.R.

    1988-05-01

    Research was conducted in a process research unit to develop an entrained bed gasifier which is supplied heat by recirculating a stream of sand between a separate combustion vessel and the gasifier. The char remaining after gasification of the wood provides the fuel for the combustor. The research program was conducted in two phases. In the first phase, a 6 in. I.D. gasifier was used to establish the feasibility of the concept for a wide variety of biomass feeds. The second phase of the program was conducted with a 10 in. I.D. gasifier, and a fully automated feeder system, to evaluate gasifier performance at very high feed rates. The experimental results were used to develop design parameters and detailed energy and material balances for a conceptual plant. A preliminary cost analysis is presented in the report based on the conceptual design. 5 refs., 24 figs., 13 tabs.

  11. Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics

    SciTech Connect (OSTI)

    Geng, Tao; Bredeweg, Erin L.; Szymanski, Craig J.; Liu, Bingwen; Baker, Scott E.; Orr, Galya; Evans, James E.; Kelly, Ryan T.

    2015-11-04

    Interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of science applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, this microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level, and quantitative dynamics of gene expression with single hyphal compartment resolution in response to carbon source starvation and exchange experiments. Although the microfluidic device is demonstrated on filamentous fungi, our technology is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth with applications ranging from bioenergy production to human health.

  12. Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics

    SciTech Connect (OSTI)

    Geng, Tao; Bredeweg, Erin L.; Szymanski, Craig J.; Liu, Bingwen; Baker, Scott E.; Orr, Galya; Evans, James E.; Kelly, Ryan T.

    2015-11-04

    Here, interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of science applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, this microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level, and quantitative dynamics of gene expression with single hyphal compartment resolution in response to carbon source starvation and exchange experiments. Although the microfluidic device is demonstrated on filamentous fungi, our technology is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth with applications ranging from bioenergy production to human health.

  13. High-Throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis

    SciTech Connect (OSTI)

    Wenwan Zhong

    2003-08-05

    Capillary electrophoresis (CE) offers many advantages over conventional analytical methods, such as speed, simplicity, high resolution, low cost, and small sample consumption, especially for the separation of enantiomers. However, chiral method developments still can be time consuming and tedious. They designed a comprehensive enantioseparation protocol employing neutral and sulfated cyclodextrins as chiral selectors for common basic, neutral, and acidic compounds with a 96-capillary array system. By using only four judiciously chosen separation buffers, successful enantioseparations were achieved for 49 out of 54 test compounds spanning a large variety of pKs and structures. Therefore, unknown compounds can be screened in this manner to identify optimal enantioselective conditions in just one rn. In addition to superior separation efficiency for small molecules, CE is also the most powerful technique for DNA separations. Using the same multiplexed capillary system with UV absorption detection, the sequence of a short DNA template can be acquired without any dye-labels. Two internal standards were utilized to adjust the migration time variations among capillaries, so that the four electropherograms for the A, T, C, G Sanger reactions can be aligned and base calling can be completed with a high level of confidence. the CE separation of DNA can be applied to study differential gene expression as well. Combined with pattern recognition techniques, small variations among electropherograms obtained by the separation of cDNA fragments produced from the total RNA samples of different human tissues can be revealed. These variations reflect the differences in total RNA expression among tissues. Thus, this Ce-based approach can serve as an alternative to the DNA array techniques in gene expression analysis.

  14. High throughput microcantilever detector

    DOE Patents [OSTI]

    Thundat, Thomas G.; Ferrell, Thomas L.; Hansen, Karolyn M.; Tian, Fang

    2004-07-20

    In an improved uncoated microcantilever detector, the sample sites are placed on a separate semi-conducting substrate and the microcantilever element detects and measures the changes before and after a chemical interaction or hybridization of the sites by sensing differences of phase angle between an alternating voltage applied to the microcantilever element and vibration of the microcantilever element. In another embodiment of the invention, multiple sample sites are on a sample array wherein an array of microcantilever elements detect and measure the change before and after chemical interactions or hybridizations of the sample sites.

  15. Robofurnace: A semi-automated laboratory chemical vapor deposition system for high-throughput nanomaterial synthesis and process discovery

    SciTech Connect (OSTI)

    Oliver, C. Ryan; Westrick, William; Koehler, Jeremy; Brieland-Shoultz, Anna; Anagnostopoulos-Politis, Ilias; Cruz-Gonzalez, Tizoc [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States)] [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States); Hart, A. John, E-mail: ajhart@mit.edu [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States); Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2013-11-15

    Laboratory research and development on new materials, such as nanostructured thin films, often utilizes manual equipment such as tube furnaces due to its relatively low cost and ease of setup. However, these systems can be prone to inconsistent outcomes due to variations in standard operating procedures and limitations in performance such as heating and cooling rates restrict the parameter space that can be explored. Perhaps more importantly, maximization of research throughput and the successful and efficient translation of materials processing knowledge to production-scale systems, relies on the attainment of consistent outcomes. In response to this need, we present a semi-automated lab-scale chemical vapor deposition (CVD) furnace system, called Robofurnace. Robofurnace is an automated CVD system built around a standard tube furnace, which automates sample insertion and removal and uses motion of the furnace to achieve rapid heating and cooling. The system has a 10-sample magazine and motorized transfer arm, which isolates the samples from the lab atmosphere and enables highly repeatable placement of the sample within the tube. The system is designed to enable continuous operation of the CVD reactor, with asynchronous loading/unloading of samples. To demonstrate its performance, Robofurnace is used to develop a rapid CVD recipe for carbon nanotube (CNT) forest growth, achieving a 10-fold improvement in CNT forest mass density compared to a benchmark recipe using a manual tube furnace. In the long run, multiple systems like Robofurnace may be linked to share data among laboratories by methods such as Twitter. Our hope is Robofurnace and like automation will enable machine learning to optimize and discover relationships in complex material synthesis processes.

  16. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomicsmore » system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.« less

  17. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    SciTech Connect (OSTI)

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomics system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.

  18. Attendees list from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    HIGH THROUGHPUT SCREENING OF HYDROGEN STORAGE MATERIALS WORKSHOP June 26, 2007 Sentech Inc., Bethesda Maryland Meeting Attendees Name Organization Mark Bailey Wildcat Discovery Technologies Leonid Bendersky NIST Theodore Besmann ORNL Larry Blair DOE Tom Boussie Symyx Technologies Bob Bowman JPL Larry Cook NIST Alan Cooper Air Products Kristin Deason Sentech Pete DeSanto Air Products R. Greg Downing NIST Mike Fasolka NIST Rick Fisher Symyx Technologies Frank Gayle NIST Ashraf Imam NRL Carter

  19. Miniaturized Analytical Platforms From Nanoparticle Components: Studies in the Construction, Characterization, and High-Throughput Usage of These Novel Architectures

    SciTech Connect (OSTI)

    Andrew David Pris

    2003-08-05

    exhibiting a variety of surface chemistries and attempts to deconvolute general adsorption rules for their assembly on various substrates. Chapter 2 extends the usage of self-assembly of polymeric nanoparticles through a layer-by-layer deposition concept and photolithography methodologies to create analytical platforms with a vertical height controlled within the nanometer regime. This platform is then furthered in Chapter 3 by employing this integrated concept as a bio-recognition platform, with the extension of the method to a high-throughput screening system explored. Chapter 4 exploits two different types of nanoparticles, silica and gold, as multiplexed, self-assembled immunoassay sensors. This final research chapter is followed by a general summation and future prospectus section that concludes the dissertation.

  20. Nanocoatings for High-Efficiency Industrial Hydraulic and Tooling Systems

    SciTech Connect (OSTI)

    Clifton B. Higdon III

    2011-01-07

    Industrial manufacturing in the U.S. accounts for roughly one third of the 98 quadrillion Btu total energy consumption. Motor system losses amount to 1.3 quadrillion Btu, which represents the largest proportional loss of any end-use category, while pumps alone represent over 574 trillion BTU (TBTU) of energy loss each year. The efficiency of machines with moving components is a function of the amount of energy lost to heat because of friction between contacting surfaces. The friction between these interfaces also contributes to downtime and the loss of productivity through component wear and subsequent repair. The production of new replacement parts requires additional energy. Among efforts to reduce energy losses, wear-resistant, low-friction coatings on rotating and sliding components offer a promising approach that is fully compatible with existing equipment and processes. In addition to lubrication, one of the most desirable solutions is to apply a protective coating or surface treatment to rotating or sliding components to reduce their friction coefficients, thereby leading to reduced wear. Historically, a number of materials such as diamond-like carbon (DLC), titanium nitride (TiN), titanium aluminum nitride (TiAlN), and tungsten carbide (WC) have been examined as tribological coatings. The primary objective of this project was the development of a variety of thin film nanocoatings, derived from the AlMgB14 system, with a focus on reducing wear and friction in both industrial hydraulics and cutting tool applications. Proof-of-concept studies leading up to this project had shown that the constituent phases, AlMgB14 and TiB2, were capable of producing low-friction coatings by pulsed laser deposition. These coatings combine high hardness with a low friction coefficient, and were shown to substantially reduce wear in laboratory tribology tests. Selection of the two applications was based largely on the concept of improved mechanical interface efficiencies for

  1. Prediction of rodent carcinogenic potential of naturally occurring chemicals in the human diet using high-throughput QSAR predictive modeling

    SciTech Connect (OSTI)

    Valerio, Luis G. . E-mail: luis.valerio@FDA.HHS.gov; Arvidson, Kirk B.; Chanderbhan, Ronald F.; Contrera, Joseph F.

    2007-07-01

    Consistent with the U.S. Food and Drug Administration (FDA) Critical Path Initiative, predictive toxicology software programs employing quantitative structure-activity relationship (QSAR) models are currently under evaluation for regulatory risk assessment and scientific decision support for highly sensitive endpoints such as carcinogenicity, mutagenicity and reproductive toxicity. At the FDA's Center for Food Safety and Applied Nutrition's Office of Food Additive Safety and the Center for Drug Evaluation and Research's Informatics and Computational Safety Analysis Staff (ICSAS), the use of computational SAR tools for both qualitative and quantitative risk assessment applications are being developed and evaluated. One tool of current interest is MDL-QSAR predictive discriminant analysis modeling of rodent carcinogenicity, which has been previously evaluated for pharmaceutical applications by the FDA ICSAS. The study described in this paper aims to evaluate the utility of this software to estimate the carcinogenic potential of small, organic, naturally occurring chemicals found in the human diet. In addition, a group of 19 known synthetic dietary constituents that were positive in rodent carcinogenicity studies served as a control group. In the test group of naturally occurring chemicals, 101 were found to be suitable for predictive modeling using this software's discriminant analysis modeling approach. Predictions performed on these compounds were compared to published experimental evidence of each compound's carcinogenic potential. Experimental evidence included relevant toxicological studies such as rodent cancer bioassays, rodent anti-carcinogenicity studies, genotoxic studies, and the presence of chemical structural alerts. Statistical indices of predictive performance were calculated to assess the utility of the predictive modeling method. Results revealed good predictive performance using this software's rodent carcinogenicity module of over 1200 chemicals

  2. OSTI search tools cited for "high quality" content | OSTI, US...

    Office of Scientific and Technical Information (OSTI)

    Thomson Scientific recently selected several OSTI search tools and Science.gov for inclusion in Current Web Contents(tm), a growing collection of scholarly Web sites. Thomson cited ...

  3. Nanocoatings for High-Efficiency Industrial Hydraulic and Tooling Systems

    SciTech Connect (OSTI)

    2009-05-01

    This factsheet describes a research project whose goal is to develop degradation-resistant nano-coatings of AlMgB14 and AlMgB14 (titanium diboride) TiB2 that result in improved surface hardness and reduced friction for industrial hydraulic and tooling systems.

  4. High Temperature Chemical Sensing Tool: Preliminary pH and reference electrode test results

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Grzegorz Cieslewski

    2014-09-30

    Project: High Temperature Chemical Sensing Tool for Distributed Mapping of Fracture Flow in EGS. Preliminary pH and reference electrode test results.

  5. High Temperature Tools and Sensors, Down-hole Pumps and Drilling

    Broader source: Energy.gov [DOE]

    Below are the project presentations and respective peer review results for High Temperature Tools and Sensors, Down-hole Pumps and Drilling.

  6. Nanocoatings for High-Efficiency Industrial and Tooling Systems

    SciTech Connect (OSTI)

    Blau, P; Qu, J.; Higdon, C.

    2011-02-01

    This industry-driven project was the result of a successful response by Eaton Corporation to a DOE/ITP Program industry call. It consisted of three phases in which ORNL participated. In addition to Eaton Corporation and ORNL (CRADA), the project team included Ames Laboratory, who developed the underlying concept for aluminum-magnesium-boron based nanocomposite coatings [1], and Greenleaf, a small tooling manufacturer in western Pennsylvania. This report focuses on the portion of this work that was conducted by ORNL in a CRADA with Eaton Corporation. A comprehensive final report for the entire effort, which ended in September 2010, has been prepared by Eaton Corporation. Phase I, Proof of Concept ran for one year (September 1, 2006 to September 30, 2007) during which the applicability of AlMgB14 single-phase and nanocomposite coatings on hydraulic material coupons and components as well as on tool inserts was demonstrated.. The coating processes used either plasma laser deposition (PLD) or physical vapor deposition (PVD). During Phase I, ORNL conducted laboratory-scale pin-on-disk and reciprocating pin-on-flat tests of coatings produced by PLD and PVD. Non-coated M2 tool steel was used as a baseline for comparison, and the material for the sliding counterface was Type 52100 bearing steel since it simulated the pump materials. Initial tests were run mainly in a commercial hydraulic fluid named Mobil DTE-24, but some tests were later run in a water-glycol mixture as well. A tribosystem analysis was conducted to define the operating conditions of pump components and to help develop simulative tests in Phase II. Phase II, Coating Process Scale-up was intended to use scaled-up process to generate prototype parts. This involved both PLD practices at Ames Lab, and a PVD scale-up study at Eaton using its production capable equipment. There was also a limited scale-up study at Greenleaf for the tooling application. ORNL continued to conduct friction and wear tests on

  7. Designing and Validating Ternary Pd Alloys for Optimum Sulfur/Carbon Resistance in Hydrogen Separation and Carbon Capture Membrane Systems Using High-Throughput Combinatorial Methods

    SciTech Connect (OSTI)

    Lewis, Amanda; Zhao, Hongbin; Hopkins, Scott

    2014-09-30

    This report summarizes the work completed under the U.S. Department of Energy Project Award No.: DE-FE0001181 titled “Designing and Validating Ternary Pd Alloys for Optimum Sulfur/Carbon Resistance in Hydrogen Separation and Carbon Capture Membrane Systems Using High-Throughput Combinatorial Methods.” The project started in October 1, 2009 and was finished September 30, 2014. Pall Corporation worked with Cornell University to sputter and test palladium-based ternary alloys onto silicon wafers to examine many alloys at once. With the specialized equipment at Georgia Institute of Technology that analyzed the wafers for adsorbed carbon and sulfur species six compositions were identified to have resistance to carbon and sulfur species. These compositions were deposited on Pall AccuSep® supports by Colorado School of Mines and then tested in simulated synthetic coal gas at the Pall Corporation. Two of the six alloys were chosen for further investigations based on their performance. Alloy reproducibility and long-term testing of PdAuAg and PdZrAu provided insight to the ability to manufacture these compositions for testing. PdAuAg is the most promising alloy found in this work based on the fabrication reproducibility and resistance to carbon and sulfur. Although PdZrAu had great initial resistance to carbon and sulfur species, the alloy composition has a very narrow range that hindered testing reproducibility.

  8. HIV-1 entry inhibition by small-molecule CCR5 antagonists: A combined molecular modeling and mutant study using a high-throughput assay

    SciTech Connect (OSTI)

    Labrecque, Jean; Metz, Markus; Lau, Gloria; Darkes, Marilyn C.; Wong, Rebecca S.Y.; Bogucki, David; Carpenter, Bryon; Chen Gang; Li Tongshuang; Nan, Susan; Schols, Dominique; Bridger, Gary J.; Fricker, Simon P.; Skerlj, Renato T.

    2011-05-10

    Based on the attrition rate of CCR5 small molecule antagonists in the clinic the discovery and development of next generation antagonists with an improved pharmacology and safety profile is necessary. Herein, we describe a combined molecular modeling, CCR5-mediated cell fusion, and receptor site-directed mutagenesis approach to study the molecular interactions of six structurally diverse compounds (aplaviroc, maraviroc, vicriviroc, TAK-779, SCH-C and a benzyloxycarbonyl-aminopiperidin-1-yl-butane derivative) with CCR5, a coreceptor for CCR5-tropic HIV-1 strains. This is the first study using an antifusogenic assay, a model of the interaction of the gp120 envelope protein with CCR5. This assay avoids the use of radioactivity and HIV infection assays, and can be used in a high throughput mode. The assay was validated by comparison with other established CCR5 assays. Given the hydrophobic nature of the binding pocket several binding models are suggested which could prove useful in the rational drug design of new lead compounds.

  9. A NOVEL LOW THERMAL BUDGET THIN-FILM POLYSILICON FABRICATION PROCESS FOR LARGE-AREA, HIGH-THROUGHPUT SOLAR CELL PRODUCTION

    SciTech Connect (OSTI)

    Yue Kuo

    2010-08-15

    A novel thin-film poly-Si fabrication process has been demonstrated. This low thermal budget process transforms the single- and multi-layer amorphous silicon thin films into a poly-Si structure in one simple step over a pulsed rapid thermal annealing process with the enhancement of an ultrathin Ni layer. The complete poly-Si solar cell was fabricated in a short period of time without deteriorating the underneath glass substrate. The unique vertical crystallization process including the mechanism is discussed. Influences of the dopant type and process parameters on crystal structure will be revealed. The poly-Si film structure has been proved using TEM, XRD, Raman, and XPS methods. The poly-Si solar cell structure and the performance have been examined. In principle, the new process is potentially applicable to produce large-area thin-film poly-Si solar cells at a high throughput and low cost. A critical issue in this process is to prevent the excessive dopant diffusion during crystallization. Process parameters and the cell structure have to be optimized to achieve the production goal.

  10. Size Matters: Assessing Optimum Soil Sample Size for Fungal and Bacterial Community Structure Analyses Using High Throughput Sequencing of rRNA Gene Amplicons

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Penton, C. Ryan; Gupta, Vadakattu V. S. R.; Yu, Julian; Tiedje, James M.

    2016-06-02

    We examined the effect of different soil sample sizes obtained from an agricultural field, under a single cropping system uniform in soil properties and aboveground crop responses, on bacterial and fungal community structure and microbial diversity indices. DNA extracted from soil sample sizes of 0.25, 1, 5, and 10 g using MoBIO kits and from 10 and 100 g sizes using a bead-beating method (SARDI) were used as templates for high-throughput sequencing of 16S and 28S rRNA gene amplicons for bacteria and fungi, respectively, on the Illumina MiSeq and Roche 454 platforms. Sample size significantly affected overall bacterial and fungalmore » community structure, replicate dispersion and the number of operational taxonomic units (OTUs) retrieved. Richness, evenness and diversity were also significantly affected. The largest diversity estimates were always associated with the 10 g MoBIO extractions with a corresponding reduction in replicate dispersion. For the fungal data, smaller MoBIO extractions identified more unclassified Eukaryota incertae sedis and unclassified glomeromycota while the SARDI method retrieved more abundant OTUs containing unclassified Pleosporales and the fungal genera Alternaria and Cercophora. Overall, these findings indicate that a 10 g soil DNA extraction is most suitable for both soil bacterial and fungal communities for retrieving optimal diversity while still capturing rarer taxa in concert with decreasing replicate variation.« less

  11. A multi-channel gel electrophoresis and continuous fraction collection apparatus for high throughput protein separation and characterization

    SciTech Connect (OSTI)

    Choi, Megan; Nordmeyer, Robert A.; Cornell, Earl; Dong, Ming; Biggin, Mark D.; Jin, Jian

    2009-10-02

    To facilitate a direct interface between protein separation by PAGE and protein identification by mass spectrometry, we developed a multichannel system that continuously collects fractions as protein bands migrate off the bottom of gel electrophoresis columns. The device was constructed using several short linear gel columns, each of a different percent acrylamide, to achieve a separation power similar to that of a long gradient gel. A Counter Free-Flow elution technique then allows continuous and simultaneous fraction collection from multiple channels at low cost. We demonstrate that rapid, high-resolution separation of a complex protein mixture can be achieved on this system using SDS-PAGE. In a 2.5 h electrophoresis run, for example, each sample was separated and eluted into 48-96 fractions over a mass range of 10-150 kDa; sample recovery rates were 50percent or higher; each channel was loaded with up to 0.3 mg of protein in 0.4 mL; and a purified band was eluted in two to three fractions (200 L/fraction). Similar results were obtained when running native gel electrophoresis, but protein aggregation limited the loading capacity to about 50 g per channel and reduced resolution.

  12. From Organized High-Throughput Data to Phenomenological Theory using Machine Learning: The Example of Dielectric Breakdown

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kim, Chiho; Pilania, Ghanshyam; Ramprasad, Ramamurthy

    2016-02-02

    Understanding the behavior (and failure) of dielectric insulators experiencing extreme electric fields is critical to the operation of present and emerging electrical and electronic devices. Despite its importance, the development of a predictive theory of dielectric breakdown has remained a challenge, owing to the complex multiscale nature of this process. We focus on the intrinsic dielectric breakdown field of insulators—the theoretical limit of breakdown determined purely by the chemistry of the material, i.e., the elements the material is composed of, the atomic-level structure, and the bonding. Starting from a benchmark dataset (generated from laborious first principles computations) of the intrinsicmore » dielectric breakdown field of a variety of model insulators, simple predictive phenomenological models of dielectric breakdown are distilled using advanced statistical or machine learning schemes, revealing key correlations and analytical relationships between the breakdown field and easily accessible material properties. Lastly, the models are shown to be general, and can hence guide the screening and systematic identification of high electric field tolerant materials.« less

  13. High power laser downhole cutting tools and systems

    DOE Patents [OSTI]

    Zediker, Mark S; Rinzler, Charles C; Faircloth, Brian O; Koblick, Yeshaya; Moxley, Joel F

    2015-01-20

    Downhole cutting systems, devices and methods for utilizing 10 kW or more laser energy transmitted deep into the earth with the suppression of associated nonlinear phenomena. Systems and devices for the laser cutting operations within a borehole in the earth. These systems and devices can deliver high power laser energy down a deep borehole, while maintaining the high power to perform cutting operations in such boreholes deep within the earth.

  14. High power laser workover and completion tools and systems

    DOE Patents [OSTI]

    Zediker, Mark S; Rinzler, Charles C; Faircloth, Brian O; Koblick, Yeshaya; Moxley, Joel F

    2014-10-28

    Workover and completion systems, devices and methods for utilizing 10 kW or more laser energy transmitted deep into the earth with the suppression of associated nonlinear phenomena. Systems and devices for the laser workover and completion of a borehole in the earth. These systems and devices can deliver high power laser energy down a deep borehole, while maintaining the high power to perform laser workover and completion operations in such boreholes deep within the earth.

  15. BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments

    SciTech Connect (OSTI)

    Round, Adam, E-mail: around@embl.fr; Felisaz, Franck [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Universit Grenoble AlpesEMBLCNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Fodinger, Lukas; Gobbo, Alexandre [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Huet, Julien [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Universit Grenoble AlpesEMBLCNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Villard, Cyril [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Blanchet, Clement E., E-mail: around@embl.fr [EMBL c/o DESY, Notkestrasse 85, 22603 Hamburg (Germany); Pernot, Petra; McSweeney, Sean [ESRF, 6 Rue Jules Horowitz, 38000 Grenoble (France); Roessle, Manfred; Svergun, Dmitri I. [EMBL c/o DESY, Notkestrasse 85, 22603 Hamburg (Germany); Cipriani, Florent, E-mail: around@embl.fr [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Universit Grenoble AlpesEMBLCNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France)

    2015-01-01

    A robotic sample changer for solution X-ray scattering experiments optimized for speed and to use the minimum amount of material has been developed. This system is now in routine use at three high-brilliance European synchrotron sites, each capable of several hundred measurements per day. Small-angle X-ray scattering (SAXS) of macromolecules in solution is in increasing demand by an ever more diverse research community, both academic and industrial. To better serve user needs, and to allow automated and high-throughput operation, a sample changer (BioSAXS Sample Changer) that is able to perform unattended measurements of up to several hundred samples per day has been developed. The Sample Changer is able to handle and expose sample volumes of down to 5 l with a measurement/cleaning cycle of under 1 min. The samples are stored in standard 96-well plates and the data are collected in a vacuum-mounted capillary with automated positioning of the solution in the X-ray beam. Fast and efficient capillary cleaning avoids cross-contamination and ensures reproducibility of the measurements. Independent temperature control for the well storage and for the measurement capillary allows the samples to be kept cool while still collecting data at physiological temperatures. The Sample Changer has been installed at three major third-generation synchrotrons: on the BM29 beamline at the European Synchrotron Radiation Facility (ESRF), the P12 beamline at the PETRA-III synchrotron (EMBL@PETRA-III) and the I22/B21 beamlines at Diamond Light Source, with the latter being the first commercial unit supplied by Bruker ASC.

  16. Biochemical & Thermochemical High Throughput Characterization...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    20 40 60 80 100 120 140 32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62 64 66 68 70 Frequency Corn Stover Corn Cob Miscanthus Wheat

  17. High throughput reproducible cantilever functionalization

    DOE Patents [OSTI]

    Evans, Barbara R; Lee, Ida

    2014-01-21

    A method for functionalizing cantilevers is provided that includes providing a holder having a plurality of channels each having a width for accepting a cantilever probe and a plurality of probes. A plurality of cantilever probes are fastened to the plurality of channels of the holder by the spring clips. The wells of a well plate are filled with a functionalization solution, wherein adjacent wells in the well plate are separated by a dimension that is substantially equal to a dimension separating adjacent channels of the plurality of channels. Each cantilever probe that is fastened within the plurality of channels of the holder is applied to the functionalization solution that is contained in the wells of the well plate.

  18. Biochemical & Thermochemical High Throughput Characterization...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    20 40 60 80 100 120 140 32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62 64 66 68 70 Frequency Corn Stover Corn Cob Miscanthus Wheat...

  19. Development of a High-Temperature Diagnostics-While-Drilling Tool

    SciTech Connect (OSTI)

    Blankenship, Douglas; Chavira, David; Henfling, Joseph; Hetmaniak, Chris; Huey, David; Jacobson, Ron; King, Dennis; Knudsen, Steve; Mansure, A. J.; Polsky, Yarom

    2009-01-01

    This report documents work performed in the second phase of the Diagnostics While-Drilling (DWD) project in which a high-temperature (HT) version of the phase 1 low-temperature (LT) proof-of-concept (POC) DWD tool was built and tested. Descriptions of the design, fabrication and field testing of the HT tool are provided.

  20. Development of a High-Temperature Diagnostics-While-Drilling Tool

    Office of Energy Efficiency and Renewable Energy (EERE)

    This report documents work performed in the second phase of the Diagnostics-While-Drilling (DWD) project in which a high-temperature (HT) version of the phase 1 low-temperature (LT) proof-of-concept (POC) DWD tool was built and tested. Descriptions of the design, fabrication and field testing of the HT tool are provided.

  1. High Energy Colliders as Tools to Understand the Early Universe

    SciTech Connect (OSTI)

    Tait, Tim

    2008-08-16

    Cosmological observations have reached a new era of precision, and reveal many interesting and puzzling features of the Universe. I will briefly review two of the most exciting mysteries: the nature of the dark components of the Universe, and the origin of the asymmetry between matter and anti-matter. I will argue that our best hope of unraveling these questions will need to combine information from the heavens with measurements in the lab at high energy particle accelerators. The end of run II of the Tevatron, the up-coming Large Hadron Collider and proposed International Linear Collider all have great potential to help us answer these questions in the near future.

  2. High-throughput prediction of Acacia and eucalypt lignin syringyl/guaiacyl content using FT-Raman spectroscopy and partial least squares modeling

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lupoi, Jason S.; Healey, Adam; Singh, Seema; Sykes, Robert; Davis, Mark; Lee, David J.; Shepherd, Merv; Simmons, Blake A.; Henry, Robert J.

    2015-01-16

    High-throughput techniques are necessary to efficiently screen potential lignocellulosic feedstocks for the production of renewable fuels, chemicals, and bio-based materials, thereby reducing experimental time and expense while supplanting tedious, destructive methods. The ratio of lignin syringyl (S) to guaiacyl (G) monomers has been routinely quantified as a way to probe biomass recalcitrance. Mid-infrared and Raman spectroscopy have been demonstrated to produce robust partial least squares models for the prediction of lignin S/G ratios in a diverse group of Acacia and eucalypt trees. The most accurate Raman model has now been used to predict the S/G ratio from 269 unknown Acaciamore » and eucalypt feedstocks. This study demonstrates the application of a partial least squares model composed of Raman spectral data and lignin S/G ratios measured using pyrolysis/molecular beam mass spectrometry (pyMBMS) for the prediction of S/G ratios in an unknown data set. The predicted S/G ratios calculated by the model were averaged according to plant species, and the means were not found to differ from the pyMBMS ratios when evaluating the mean values of each method within the 95 % confidence interval. Pairwise comparisons within each data set were employed to assess statistical differences between each biomass species. While some pairwise appraisals failed to differentiate between species, Acacias, in both data sets, clearly display significant differences in their S/G composition which distinguish them from eucalypts. In conclusion, this research shows the power of using Raman spectroscopy to supplant tedious, destructive methods for the evaluation of the lignin S/G ratio of diverse plant biomass materials.« less

  3. High-throughput prediction of Acacia and eucalypt lignin syringyl/guaiacyl content using FT-Raman spectroscopy and partial least squares modeling

    SciTech Connect (OSTI)

    Lupoi, Jason S.; Healey, Adam; Singh, Seema; Sykes, Robert; Davis, Mark; Lee, David J.; Shepherd, Merv; Simmons, Blake A.; Henry, Robert J.

    2015-01-16

    High-throughput techniques are necessary to efficiently screen potential lignocellulosic feedstocks for the production of renewable fuels, chemicals, and bio-based materials, thereby reducing experimental time and expense while supplanting tedious, destructive methods. The ratio of lignin syringyl (S) to guaiacyl (G) monomers has been routinely quantified as a way to probe biomass recalcitrance. Mid-infrared and Raman spectroscopy have been demonstrated to produce robust partial least squares models for the prediction of lignin S/G ratios in a diverse group of Acacia and eucalypt trees. The most accurate Raman model has now been used to predict the S/G ratio from 269 unknown Acacia and eucalypt feedstocks. This study demonstrates the application of a partial least squares model composed of Raman spectral data and lignin S/G ratios measured using pyrolysis/molecular beam mass spectrometry (pyMBMS) for the prediction of S/G ratios in an unknown data set. The predicted S/G ratios calculated by the model were averaged according to plant species, and the means were not found to differ from the pyMBMS ratios when evaluating the mean values of each method within the 95 % confidence interval. Pairwise comparisons within each data set were employed to assess statistical differences between each biomass species. While some pairwise appraisals failed to differentiate between species, Acacias, in both data sets, clearly display significant differences in their S/G composition which distinguish them from eucalypts. In conclusion, this research shows the power of using Raman spectroscopy to supplant tedious, destructive methods for the evaluation of the lignin S/G ratio of diverse plant biomass materials.

  4. High-Energy X-Ray Diffraction Analysis Tool

    Energy Science and Technology Software Center (OSTI)

    2011-11-29

    The functionality of heRXD includes the following: distance and angular calibration and viewing flat-panel detector images used for X-ray diffraction; image (polar) rebinning or "caking"; line position fitting in powder diffraction images; image segmentation or "blob finding"; crystal orentation indesing; and lattice vector refinement. These functionalities encompass a critical set analyzing teh data for high-energy diffraction measurements that are currently performed at synchrotron sources such as the Advanced Photon Source (APS). The software design modularmore » and open source under LGPL. The intent is to provide a common framework and graphical user interface that has the ability to utillize internal as well as external subroutines to provide various optins for performing the fuctionalities listed above. The software will initially be deployed at several national user facilities--including APS, ALS, and CHESS--and then made available for download using a hosting service such as sourceforge.« less

  5. Improved Modeling Tools Development for High Penetration Solar

    SciTech Connect (OSTI)

    Washom, Byron

    2014-12-11

    One of the significant objectives of the High Penetration solar research is to help the DOE understand, anticipate, and minimize grid operation impacts as more solar resources are added to the electric power system. For Task 2.2, an effective, reliable approach to predicting solar energy availability for energy generation forecasts using the University of California, San Diego (UCSD) Sky Imager technology has been demonstrated. Granular cloud and ramp forecasts for the next 5 to 20 minutes over an area of 10 square miles were developed. Sky images taken every 30 seconds are processed to determine cloud locations and cloud motion vectors yielding future cloud shadow locations respective to distributed generation or utility solar power plants in the area. The performance of the method depends on cloud characteristics. On days with more advective cloud conditions, the developed method outperforms persistence forecasts by up to 30% (based on mean absolute error). On days with dynamic conditions, the method performs worse than persistence. Sky Imagers hold promise for ramp forecasting and ramp mitigation in conjunction with inverter controls and energy storage. The pre-commercial Sky Imager solar forecasting algorithm was documented with licensing information and was a Sunshot website highlight

  6. Tools for Enhanced Grid Operation and Optimized PV Penetration Utilizing Highly Distributed Sensor Data.

    SciTech Connect (OSTI)

    Reno, Matthew J.; Peppanen, Jouni; Seuss, John; Lave, Matthew Samuel; Broderick, Robert Joseph; Grijalva, Santiago

    2015-11-01

    Increasing number s of PV on distribution systems are creating more grid impacts , but it also provides more opportunities for measurement, sensing, and control of the grid in a distributed fashion. This report demonstrates three software tools for characterizing and controlling distribution feeders by utilizing large numbers of highly distributed current, voltage , and irradiance sensors. Instructions and a user manual is presented for each tool. First, the tool for distribution system secondary circuit parameter estimation is presented. This tool allows studying distribution system parameter estimation accuracy with user-selected active power, reactive power, and voltage measurements and measurement error levels. Second, the tool for multi-objective inverter control is shown. Various PV inverter control strategies can be selected to objectively compare their impact on the feeder. Third, the tool for energy storage for PV ramp rate smoothing is presented. The tool allows the user to select different storage characteristics (power and energy ratings) and control types (local vs. centralized) to study the tradeoffs between state-of-charge (SOC) management and the amount of ramp rate smoothing.

  7. Development Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    More Python Tools Python is an interpreted, general-purpose high-level programming language that is usually accompanied by a variety of useful tools for scientific programming...

  8. Driving Down HB-LED Costs. Implementation of Process Simulation Tools and Temperature Control Methods of High Yield MOCVD Growth

    SciTech Connect (OSTI)

    Quinn, William

    2012-04-30

    The overall objective of this multi-faceted program is to develop epitaxial growth systems that meet a goal of 75% (4X) cost reduction in the epitaxy phase of HB-LED manufacture. A 75% reduction in yielded epitaxy cost is necessary in order to achieve the cost goals for widespread penetration of HB-LED's into back-lighting units (BLU) for LCD panels and ultimately for solid-state lighting (SSL). To do this, the program will address significant improvements in overall equipment Cost of Ownership, or CoO. CoO is a model that includes all costs associated with the epitaxy portion of production. These aspects include cost of yield, capital cost, operational costs, and maintenance costs. We divide the program into three phases where later phases will incorporate the gains of prior phases. Phase one activities are enabling technologies. In collaboration with Sandia National Laboratories we develop a Fluent-compatible chemistry predictive model and a set of mid-infrared and near-ultraviolet pyrometer monitoring tools. Where previously the modeling of the reactor dynamics were studied within FLUENT alone, here, FLUENT and Chemkin are integrated into a comprehensive model of fluid dynamics and the most advanced transport equations developed for Chemkin. Specifically, the Chemkin model offered the key reaction terms for gas-phase nucleation, a key consideration in the optimization of the MOCVD process. This new predictive model is used to design new MOCVD reactors with optimized growth conditions and the newly developed pyrometers are used monitor and control the MOCVD process temperature to within 0.5°C run-to-run and within each wafer. This portion of the grant is in collaboration with partners at Sandia National Laboratories. Phase two activities are continuous improvement projects which extend the current reactor platform along the lines of improved operational efficiency, improved systems control for throughput, and carrier modifications for increased yield

  9. CgWind: A high-order accurate simulation tool for wind turbines and wind farms

    SciTech Connect (OSTI)

    Chand, K K; Henshaw, W D; Lundquist, K A; Singer, M A

    2010-02-22

    CgWind is a high-fidelity large eddy simulation (LES) tool designed to meet the modeling needs of wind turbine and wind park engineers. This tool combines several advanced computational technologies in order to model accurately the complex and dynamic nature of wind energy applications. The composite grid approach provides high-quality structured grids for the efficient implementation of high-order accurate discretizations of the incompressible Navier-Stokes equations. Composite grids also provide a natural mechanism for modeling bodies in relative motion and complex geometry. Advanced algorithms such as matrix-free multigrid, compact discretizations and approximate factorization will allow CgWind to perform highly resolved calculations efficiently on a wide class of computing resources. Also in development are nonlinear LES subgrid-scale models required to simulate the many interacting scales present in large wind turbine applications. This paper outlines our approach, the current status of CgWind and future development plans.

  10. GeoChip 3.0 as a high-thoughput tool for analyzing microbial community composition, structure, and functional activity

    SciTech Connect (OSTI)

    He, Z.; Deng, Y.; Van Nostrand, J.D.; Tu, Q.; Xu, M.; Hemme, C.L.; Li, X.; Wu, L.; Gentry, T.J.; Yin, Y.; Liebich, J.; Hazen, T.C.; Zhou, J.

    2010-04-01

    A new generation of functional gene arrays (FGAs; GeoChip 3.0) has been developed, with {approx}28,000 probes covering approximately 57,000 gene variants from 292 functional gene families involved in carbon, nitrogen, phosphorus and sulfur cycles, energy metabolism, antibiotic resistance, metal resistance and organic contaminant degradation. GeoChip 3.0 also has several other distinct features, such as a common oligo reference standard (CORS) for data normalization and comparison, a software package for data management and future updating and the gyrB gene for phylogenetic analysis. Computational evaluation of probe specificity indicated that all designed probes would have a high specificity to their corresponding targets. Experimental analysis with synthesized oligonucleotides and genomic DNAs showed that only 0.0036-0.025% false-positive rates were observed, suggesting that the designed probes are highly specific under the experimental conditions examined. In addition, GeoChip 3.0 was applied to analyze soil microbial communities in a multifactor grassland ecosystem in Minnesota, USA, which showed that the structure, composition and potential activity of soil microbial communities significantly changed with the plant species diversity. As expected, GeoChip 3.0 is a high-throughput powerful tool for studying microbial community functional structure, and linking microbial communities to ecosystem processes and functioning.

  11. SOWFA Super-Controller: A High-Fidelity Tool for Evaluating Wind Plant Control Approaches

    SciTech Connect (OSTI)

    Fleming, P.; Gebraad, P.; van Wingerden, J. W.; Lee, S.; Churchfield, M.; Scholbrock, A.; Michalakes, J.; Johnson, K.; Moriarty, P.

    2013-01-01

    This paper presents a new tool for testing wind plant controllers in the Simulator for Offshore Wind Farm Applications (SOWFA). SOWFA is a high-fidelity simulator for the interaction between wind turbine dynamics and the fluid flow in a wind plant. The new super-controller testing environment in SOWFA allows for the implementation of the majority of the wind plant control strategies proposed in the literature.

  12. Nuclear fuel cycle system simulation tool based on high-fidelity component modeling

    SciTech Connect (OSTI)

    Ames, David E.

    2014-02-01

    The DOE is currently directing extensive research into developing fuel cycle technologies that will enable the safe, secure, economic, and sustainable expansion of nuclear energy. The task is formidable considering the numerous fuel cycle options, the large dynamic systems that each represent, and the necessity to accurately predict their behavior. The path to successfully develop and implement an advanced fuel cycle is highly dependent on the modeling capabilities and simulation tools available for performing useful relevant analysis to assist stakeholders in decision making. Therefore a high-fidelity fuel cycle simulation tool that performs system analysis, including uncertainty quantification and optimization was developed. The resulting simulator also includes the capability to calculate environmental impact measures for individual components and the system. An integrated system method and analysis approach that provides consistent and comprehensive evaluations of advanced fuel cycles was developed. A general approach was utilized allowing for the system to be modified in order to provide analysis for other systems with similar attributes. By utilizing this approach, the framework for simulating many different fuel cycle options is provided. Two example fuel cycle configurations were developed to take advantage of used fuel recycling and transmutation capabilities in waste management scenarios leading to minimized waste inventories.

  13. A High Performance Computing Platform for Performing High-Volume Studies With Windows-based Power Grid Tools

    SciTech Connect (OSTI)

    Chen, Yousu; Huang, Zhenyu

    2014-08-31

    Serial Windows-based programs are widely used in power utilities. For applications that require high volume simulations, the single CPU runtime can be on the order of days or weeks. The lengthy runtime, along with the availability of low cost hardware, is leading utilities to seriously consider High Performance Computing (HPC) techniques. However, the vast majority of the HPC computers are still Linux-based and many HPC applications have been custom developed external to the core simulation engine without consideration for ease of use. This has created a technical gap for applying HPC-based tools to todays power grid studies. To fill this gap and accelerate the acceptance and adoption of HPC for power grid applications, this paper presents a prototype of generic HPC platform for running Windows-based power grid programs on Linux-based HPC environment. The preliminary results show that the runtime can be reduced from weeks to hours to improve work efficiency.

  14. Development of a high-temperature diagnostics-while-drilling tool.

    SciTech Connect (OSTI)

    Chavira, David J.; Huey, David; Hetmaniak, Chris; Polsky, Yarom; King, Dennis K.; Jacobson, Ronald David; Blankenship, Douglas Alan; Knudsen, Steven Dell; Henfling, Joseph Anthony; Mansure, Arthur James

    2009-01-01

    The envisioned benefits of Diagnostics-While-Drilling (DWD) are based on the principle that high-speed, real-time information from the downhole environment will promote better control of the drilling process. Although in practice a DWD system could provide information related to any aspect of exploration and production of subsurface resources, the current DWD system provides data on drilling dynamics. This particular set of new tools provided by DWD will allow quicker detection of problems, reduce drilling flat-time and facilitate more efficient drilling (drilling optimization) with the overarching result of decreased drilling costs. In addition to providing the driller with an improved, real-time picture of the drilling conditions downhole, data generated from DWD systems provides researchers with valuable, high fidelity data sets necessary for developing and validating enhanced understanding of the drilling process. Toward this end, the availability of DWD creates a synergy with other Sandia Geothermal programs, such as the hard-rock bit program, where the introduction of alternative rock-reduction technologies are contingent on the reduction or elimination of damaging dynamic effects. More detailed descriptions of the rationale for the program and early development efforts are described in more detail by others [SAND2003-2069 and SAND2000-0239]. A first-generation low-temperature (LT) DWD system was fielded in a series of proof-of-concept tests (POC) to validate functionality. Using the LT system, DWD was subsequently used to support a single-laboratory/multiple-partner CRADA (Cooperative Research and Development Agreement) entitled Advanced Drag Bits for Hard-Rock Drilling. The drag-bit CRADA was established between Sandia and four bit companies, and involved testing of a PDC bit from each company [Wise, et al., 2003, 2004] in the same lithologic interval at the Gas Technology Institute (GTI) test facility near Catoosa, OK. In addition, the LT DWD system has

  15. Application of a High-Throughput Analyzer in Evaluating Solid Adsorbents for Post-Combustion Carbon Capture via Multicomponent Adsorption of CO2, N-2, and H2O

    SciTech Connect (OSTI)

    Mason, JA; McDonald, TM; Bae, TH; Bachman, JE; Sumida, K; Dutton, JJ; Kaye, SS; Long, JR

    2015-04-15

    Despite the large number of metal-organic frameworks that have been studied in the context of post-combustion carbon capture, adsorption equilibria of gas mixtures including CO2, N-2, and H2O, which are the three biggest components of the flue gas emanating from a coal- or natural gas-fired power plant, have never been reported. Here, we disclose the design and validation of a high-throughput multicomponent adsorption instrument that can measure equilibrium adsorption isotherms for mixtures of gases at conditions that are representative of an actual flue gas from a power plant. This instrument is used to study 15 different metal-organic frameworks, zeolites, mesoporous silicas, and activated carbons representative of the broad range of solid adsorbents that have received attention for CO2 capture. While the multicomponent results presented in this work provide many interesting fundamental insights, only adsorbents functionalized with alkylamines are shown to have any significant CO2 capacity in the presence of N-2 and H2O at equilibrium partial pressures similar to those expected in a carbon capture process. Most significantly, the amine-appended metal organic framework mmen-Mg-2(dobpdc) (mmen = N,N'-dimethylethylenediamine, dobpdc (4-) = 4,4'-dioxido-3,3'-biphenyldicarboxylate) exhibits a record CO2 capacity of 4.2 +/- 0.2 mmol/g (16 wt %) at 0.1 bar and 40 degrees C in the presence of a high partial pressure of H2O.

  16. High Throughput Materials Characterization John M. Gregoire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Alpha N'Diaye JCAP 3 : John Gregoire, Santosh Suram, Misha Pesenson, Junko Yano, Frances Houle CMI 4 : Matt Kramer JCESR 5 : Venkat Srinivasan Kristin Persson (MP 6 ), Tieren...

  17. Unconventional Architectures for High-Throughput Sciences

    SciTech Connect (OSTI)

    Nieplocha, Jarek; Marquez, Andres; Petrini, Fabrizio; Chavarría-Miranda, Daniel

    2007-06-15

    Science laboratories and sophisticated simulations are producing data of increasing volumes and complexities, and that’s posing significant challenges to current data infrastructures as terabytes to petabytes of data must be processed and analyzed. Traditional computing platforms, originally designed to support model-driven applications, are unable to meet the demands of the data-intensive scientific applications. Pacific Northwest National Laboratory (PNNL) research goes beyond “traditional supercomputing” applications to address emerging problems that need scalable, real-time solutions. The outcome is new unconventional architectures for data-intensive applications specifically designed to process the deluge of scientific data, including FPGAs, multithreaded architectures and IBM's Cell.

  18. High throughput solar cell ablation system

    DOE Patents [OSTI]

    Harley, Gabriel; Pass, Thomas; Cousins, Peter John; Viatella, John

    2012-09-11

    A solar cell is formed using a solar cell ablation system. The ablation system includes a single laser source and several laser scanners. The laser scanners include a master laser scanner, with the rest of the laser scanners being slaved to the master laser scanner. A laser beam from the laser source is split into several laser beams, with the laser beams being scanned onto corresponding wafers using the laser scanners in accordance with one or more patterns. The laser beams may be scanned on the wafers using the same or different power levels of the laser source.

  19. High-throughput proteomics : optical approaches.

    SciTech Connect (OSTI)

    Davidson, George S.

    2008-09-01

    Realistic cell models could greatly accelerate our ability to engineer biochemical pathways and the production of valuable organic products, which would be of great use in the development of biofuels, pharmaceuticals, and the crops for the next green revolution. However, this level of engineering will require a great deal more knowledge about the mechanisms of life than is currently available. In particular, we need to understand the interactome (which proteins interact) as it is situated in the three dimensional geometry of the cell (i.e., a situated interactome), and the regulation/dynamics of these interactions. Methods for optical proteomics have become available that allow the monitoring and even disruption/control of interacting proteins in living cells. Here, a range of these methods is reviewed with respect to their role in elucidating the interactome and the relevant spatial localizations. Development of these technologies and their integration into the core competencies of research organizations can position whole institutions and teams of researchers to lead in both the fundamental science and the engineering applications of cellular biology. That leadership could be particularly important with respect to problems of national urgency centered around security, biofuels, and healthcare.

  20. High throughput solar cell ablation system

    DOE Patents [OSTI]

    Harley, Gabriel; Pass, Thomas; Cousins, Peter John; Viatella, John

    2014-10-14

    A solar cell is formed using a solar cell ablation system. The ablation system includes a single laser source and several laser scanners. The laser scanners include a master laser scanner, with the rest of the laser scanners being slaved to the master laser scanner. A laser beam from the laser source is split into several laser beams, with the laser beams being scanned onto corresponding wafers using the laser scanners in accordance with one or more patterns. The laser beams may be scanned on the wafers using the same or different power levels of the laser source.

  1. High throughput liquid absorption preconcentrator sampling instrument

    DOE Patents [OSTI]

    Zaromb, S.; Bozen, R.M.

    1992-12-22

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis. 12 figs.

  2. Constant pressure high throughput membrane permeation testing...

    Office of Scientific and Technical Information (OSTI)

    Authors: Albenze, Erik J. ; Hopkinson, David P. ; Luebke, David R. Publication Date: 2014-09-02 OSTI Identifier: 1156820 Report Number(s): 8,821,614 13629,733 Resource Type: ...

  3. High throughput liquid absorption preconcentrator sampling instrument

    DOE Patents [OSTI]

    Zaromb, Solomon (Hinsdale, IL); Bozen, Ralph M. (Hattiesburg, MS)

    1992-01-01

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis.

  4. Ensemble Jobs for Better Throughput - Videoconference | Argonne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ensemble Jobs for Better Throughput - Videoconference Event Sponsor: Argonne National Laboratory Start Date: Sep 24 2015 - 1:00pm BuildingRoom: Online Videoconference Location:...

  5. Final Project Report: Tools for the Development of High Performance Energy Applications and Systems

    SciTech Connect (OSTI)

    Hollingsworth, Jeff

    2014-10-22

    This project concentrated on various aspects of creating tool infrastructure to make it easier to program large-scale parallel computers. The research conducted during this project is summarized in this report. The complete details of the work are available in the ten publications listed at the end of the report. Many of the concepts created during this project have been incorporated into tools and made available as freely downloadable software (at www.dyninst.org). It also supported the Ph.D. studies of three students.

  6. Algal functional annotation tool

    SciTech Connect (OSTI)

    2012-07-12

    pathway maps and batch gene identifier conversion. CONCLUSIONS: The Algal Functional Annotation Tool aims to provide an integrated data-mining environment for algal genomics by combining data from multiple annotation databases into a centralized tool. This site is designed to expedite the process of functional annotation and the interpretation of gene lists, such as those derived from high-throughput RNA-seq experiments. The tool is publicly available at http://pathways.mcdb.ucla.edu.

  7. Algal functional annotation tool

    Energy Science and Technology Software Center (OSTI)

    2012-07-12

    KEGG pathway maps and batch gene identifier conversion. CONCLUSIONS: The Algal Functional Annotation Tool aims to provide an integrated data-mining environment for algal genomics by combining data from multiple annotation databases into a centralized tool. This site is designed to expedite the process of functional annotation and the interpretation of gene lists, such as those derived from high-throughput RNA-seq experiments. The tool is publicly available at http://pathways.mcdb.ucla.edu.« less

  8. Rapid Damage Assessment Using High-resolution Remote Sensing Imagery: Tools and Techniques

    SciTech Connect (OSTI)

    Vatsavai, Raju; Tuttle, Mark A; Bhaduri, Budhendra L; Bright, Eddie A; Cheriyadat, Anil M; Chandola, Varun; Graesser, Jordan B

    2011-01-01

    Accurate damage assessment caused by major natural and anthropogenic disasters is becoming critical due to increases in human and economic loss. This increase in loss of life and severe damages can be attributed to growing population, as well as human migration to disaster prone regions of the world. Rapid damage assessment and dissemination of accurate information is critical for creating an effective emergency response. Remote sensing and geographic information systems (GIS) based techniques and tools are important in disaster damage assessment and reporting activities. In this review, we will look into the state of the art techniques in damage assessment using remote sensing and GIS.

  9. Regulatory Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regulatory Tools Home CRA - 2004 Final Recertification Decision CRA Comments & Responses CCA - 1996 CRA CARDs & TSDs CCA CARDs & TSDs Regulatory Tools Title 40 CFR Part 191 Environmental Radiation Protection Standards for Management and Disposal of Spent Nuclear Fuel, High-Level and Transuranic Radioactive Wastes. Title 40 CFR Part 194 Criteria for the Certification and Re-Certification of the Waste Isolation Pilot Plant's Compliance With the 40 CFR Part 191 Disposal Regulations.

  10. Active wear and failure mechanisms of TiN-coated high speed steel and TiN-coated cemented carbide tools when machining powder metallurgically made stainless steels

    SciTech Connect (OSTI)

    Jiang, L.; Haenninen, H.; Paro, J.; Kauppinen, V.

    1996-09-01

    In this study, active wear and failure mechanisms of both TiN-coated high speed steel and TiN-coated cemented carbide tools when machining stainless steels made by powder metallurgy in low and high cutting speed ranges, respectively, have been investigated. Abrasive wear mechanisms, fatigue-induced failure, and adhesive and diffusion wear mechanisms mainly affected the tool life of TiN-coated high speed steel tools at cutting speeds below 35 m/min, between 35 and 45 m/min, and over 45 m/min, respectively. Additionally, fatigue-induced failure was active at cutting speeds over 45 m/min in the low cutting speed range when machining powder metallurgically made duplex stainless steel 2205 and austenitic stainless steel 316L. In the high cutting speed range, from 100 to 250 m/min, fatigue-induced failure together with diffusion wear mechanism, affected the tool life of TiN-coated cemented carbide tools when machining both 316L and 2205 stainless steels. It was noticed that the tool life of TiN-coated high speed steel tools used in the low cutting speed range when machining 2205 steel was longer than that when machining 316L steel, whereas the tool life of TiN-coated cemented carbide tools used in the high cutting speed range when machining 316L steel was longer than that when machining 2205 steel.

  11. Conformance Tool High Level Design Document: IEC 61850 Cyber Security Acceleration Project

    SciTech Connect (OSTI)

    Edgar, Thomas W.

    2013-05-01

    This document is the high level design document for the Pacific Northwest National Laboratory (PNNL) IEC 62351-3, 4 and 6 standards conformance test software toolkit.

  12. Development of a Chemoenzymatic-like and Photoswitchable Method for the High-Throughput creation of Protein Microarrays. Application to the Analysis of the Protein/Protein Interactions Involved in the YOP Virulon from Yersinia pestis.

    SciTech Connect (OSTI)

    Camarero, J A

    2006-12-07

    Protein arrays are ideal tools for the rapid analysis of whole proteomes as well as for the development of reliable and cheap biosensors. The objective of this proposal is to develop a new ligand assisted ligation method based in the naturally occurring protein trans-splicing process. This method has been used for the generation of spatially addressable arrays of multiple protein components by standard micro-lithographic techniques. Key to our approach is the use of the protein trans-splicing process. This naturally occurring process allows the development of a truly generic and highly efficient method for the covalent attachment of proteins through its C-terminus to any solid support. This technology has been used for the creation of protein chips containing several virulence factors from the human pathogen Y. pestis.

  13. Open access to high-level data and analysis tools in the CMS experiment at the LHC

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Calderon, A.; Colling, D.; Huffman, A.; Lassila-Perini, K.; McCauley, T.; Rao, A.; Rodriguez-Marrero, A.; Sexton-Kennedy, E.

    2015-12-23

    The CMS experiment, in recognition of its commitment to data preservation and open access as well as to education and outreach, has made its first public release of high-level data under the CC0 waiver: up to half of the proton-proton collision data (by volume) at 7 TeV from 2010 in CMS Analysis Object Data format. CMS has prepared, in collaboration with CERN and the other LHC experiments, an open-data web portal based on Invenio. The portal provides access to CMS public data as well as to analysis tools and documentation for the public. The tools include an event display andmore » histogram application that run in the browser. In addition a virtual machine containing a CMS software environment along with XRootD access to the data is available. Within the virtual machine the public can analyse CMS data, example code is provided. As a result, we describe the accompanying tools and documentation and discuss the first experiences of data use.« less

  14. Open access to high-level data and analysis tools in the CMS experiment at the LHC

    SciTech Connect (OSTI)

    Calderon, A.; Colling, D.; Huffman, A.; Lassila-Perini, K.; McCauley, T.; Rao, A.; Rodriguez-Marrero, A.; Sexton-Kennedy, E.

    2015-12-23

    The CMS experiment, in recognition of its commitment to data preservation and open access as well as to education and outreach, has made its first public release of high-level data under the CC0 waiver: up to half of the proton-proton collision data (by volume) at 7 TeV from 2010 in CMS Analysis Object Data format. CMS has prepared, in collaboration with CERN and the other LHC experiments, an open-data web portal based on Invenio. The portal provides access to CMS public data as well as to analysis tools and documentation for the public. The tools include an event display and histogram application that run in the browser. In addition a virtual machine containing a CMS software environment along with XRootD access to the data is available. Within the virtual machine the public can analyse CMS data, example code is provided. As a result, we describe the accompanying tools and documentation and discuss the first experiences of data use.

  15. Calibration and Collaboration: Important Tools to Design high-Performance Affordable Buildings

    SciTech Connect (OSTI)

    Jiang, Wei; Liu, Bing; Snell, John; Helmes, Dan

    2008-03-31

    When new technologies are installed in a building, it is difficult to know how various systems will interact and if the building will perform as well as expected. A widely used technique to verify and quantify the actual energy savings from the energy-efficient features in high-performance buildings is to use the calibrated energy simulation approach. Maverick Gardens Mid-Rise A is a six-story apartment building located in East Boston, Massachusetts. The building was designed and constructed to meet the ENERGY STAR Homes Program rating and the U.S. Green Building Councils Leadership in Energy and Environmental Design (LEED) certification. During the design phase, DOE-2.1E energy models for both budget building design and proposed building design were developed by the design team to demonstrate energy savings potential from various energy efficient technologies installed in this high-performance building. When comparing the energy use predicted by the proposed design energy model with utility bills, the design team observed that this buildings actual energy consumption was about one-third of what was estimated from the proposed design model, and therefore requested help from the authors through the U.S. Department of Energys Rebuild America Program to calibrate the proposed design energy model. This paper describes the energy simulation calibration approach using short-term metering data and utility bills. Details of the analysis, calibration results and the actual building energy performance are presented. This study also discusses lessons learned during the simulation calibration process and demonstrates the importance of collaboration among design professionals throughout the design, building, and commissioning process, as a way to ensure that high-performing building goals are met.

  16. TU-C-BRE-02: A Novel, Highly Efficient and Automated Quality Assurance Tool for Modern Linear Accelerators

    SciTech Connect (OSTI)

    Goddu, S; Sun, B; Yaddanapudi, S; Kamal, G; Mutic, S; Baltes, C; Rose, S; Stinson, K

    2014-06-15

    Purpose: Quality assurance (QA) of complex linear accelerators is critical and highly time consuming. Varians Machine Performance Check (MPC) uses IsoCal phantom to test geometric and dosimetric aspects of the TrueBeam systems in <5min. In this study we independently tested the accuracy and robustness of the MPC tools. Methods: MPC is automated for simultaneous image-acquisition, using kV-and-MV onboard-imagers (EPIDs), while delivering kV-and-MV beams in a set routine of varying gantry, collimator and couch angles. MPC software-tools analyze the images to test: i) beam-output and uniformity, ii) positional accuracy of isocenter, EPIDs, collimating jaws (CJs), MLC leaves and couch and iii) rotational accuracy of gantry, collimator and couch. 6MV-beam dose-output and uniformity were tested using ionization-chamber (IC) and ICarray. Winston-Lutz-Tests (WLT) were performed to measure isocenter-offsets caused by gantry, collimator and couch rotations. Positional accuracy of EPIDs was evaluated using radio-opaque markers of the IsoCal phantom. Furthermore, to test the robustness of the MPC tools we purposefully miscalibrated a non-clinical TrueBeam by introducing errors in beam-output, energy, symmetry, gantry angle, couch translations, CJs and MLC leaves positions. Results: 6MV-output and uniformity were within 0.6% for most measurements with a maximum deviation of 1.0%. Average isocenter-offset caused by gantry and collimator rotations was 0.3160.011mm agreeing with IsoLock (0.274mm) and WLT (0.41mm). Average rotation-induced couch-shift from MPC was 0.3780.032mm agreeing with WLT (0.35mm). MV-and-kV imager-offsets measured by MPC were within 0.15mm. MPC predicted all machine miscalibrations within acceptable clinical tolerance. MPC detected the output miscalibrations within 0.61% while the MLC and couch positions were within 0.06mm and 0.14mm, respectively. Gantry angle miscalibrations were detected within 0.1. Conclusions: MPC is a useful tool for QA of

  17. Strong gravitational lensing as a tool to investigate the structure of jets at high energies

    SciTech Connect (OSTI)

    Barnacka, Anna; Geller, Margaret J.; Benbow, Wystan; Dell'antonio, Ian P.

    2014-06-20

    The components of blazar jets that emit radiation span a factor of 10{sup 10} in scale. The spatial structure of these emitting regions depends on the observed energy. Photons emitted at different sites cross the lens plane at different distances from the mass-weighted center of the lens. Thus there are differences in magnification ratios and time delays between the images of lensed blazars observed at different energies. When the lens structure and redshift are known from optical observations, these constraints can elucidate the structure of the source at high energies. At these energies, current technology is inadequate to resolve these sources, and the observed light curve is thus the sum of the images. Durations of ?-ray flares are short compared with typical time delays; thus both the magnification ratio and the time delay can be measured for the delayed counterparts. These measurements are a basis for localizing the emitting region along the jet. To demonstrate the power of strong gravitational lensing, we build a toy model based on the best studied and the nearest relativistic jet M87.

  18. Interactive Activity Detection Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Activity Detection Tools Interactive Activity Detection Tools Tools for detecting specified activities in video data provide a key intelligence capability. High numbers of false alarms, however, reduce tool effectiveness and analyst patience. User feedback reduces false alarms * This project will reduce the number of false alarms generated by activity detection tools (including single vehicle start / stop, multi-vehicle meetings and coordinated driving patterns) by exploiting user feedback in a

  19. High Througput Combinatorial Techniques in Hydrogen Storage Materials R&D Workshop

    Office of Energy Efficiency and Renewable Energy (EERE)

    Summary of the June 2007 high-throughput combinatorial techniques in hyrogen storage materials workshop

  20. Software Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Software Tools Software Tools Copyright materials are released under different mechanisms: Open Source Software and Portfolio Software. CONTACT thumbnail of Kathleen McDonald Head ...

  1. Enrollment Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Enrollment Tools Enrollment Tools A comprehensive benefits package with plan options for health care and retirement to take care of our employees today and tomorrow. Contacts Benefits Office (505) 667-1806 Email Enrollment Tools New Hire Benefits Checklist (pdf) New Hire Enrollment Tool (xls

  2. Percussion tool

    DOE Patents [OSTI]

    Reed, Teddy R.

    2006-11-28

    A percussion tool is described and which includes a housing mounting a tool bit; a reciprocally moveable hammer borne by the housing and which is operable to repeatedly strike the tool bit; and a reciprocally moveable piston enclosed within the hammer and which imparts reciprocal movement to the reciprocally moveable hammer.

  3. Surface Quality of Ti-6%Al-4%V ELI When Machined Using CVD-Carbide Tools at High Cutting Speed

    SciTech Connect (OSTI)

    Gusri, A. I.; Che Hassan, C. H.; Jaharah, A. G.; Yasir, A.; Zaid, Y.; Yanuar, B.

    2011-01-17

    Machining of Ti-6Al-4V ELI becomes more interested topic due to extremely weight-to-strength ratio and resistance to corrosion at elevated temperature. Quality of machined surface is presented by surface roughness, surface texture and damages of microstructure of titanium alloys. The turning parameters evaluated are cutting speed of 55-95 m/min, feed rate of 0.15-0.35 mm/rev, depth of cut of 0.10-0.20 mm and tool grade of CVD carbide tools. The results show the trend lines of surface roughness value are higher at the initial machining and the surface texture profile has a strong correlation with the feed rate. At the machining condition of cutting speed of 95 m/min, feed rate of 0.35 mm/rev and depth of cut of 0.10 mm produced the with layer with thickness of 2.0 {mu}m.

  4. Technology Transfer Webinar on November 12: High-Performance Hybrid Simulation/Measurement-Based Tools for Proactive Operator Decision-Support

    Broader source: Energy.gov [DOE]

    DOE/OE and EPRI will host a technology transfer webinar on Wednesday, November 12, 2014 from noon to 2 p.m. (ET). The purpose of this open webinar is to disseminate results and outcomes of the recently completed project “High-Performance Hybrid Simulation/Measurement-Based Tools for Proactive Operator Decision-Support,” which is one of the awarded projects of the DOE Advanced Modeling Grid Research Program.

  5. Veeco Develops Tools to Drive Down HBLED Costs

    Broader source: Energy.gov [DOE]

    Veeco is working in partnership with Sandia National Laboratories and Philips Lumileds to drive down the cost of high-brightness LEDs by implementing process simulation tools and by improving temperature measurement and control methods to increase MOCVD yield. Veeco is working with Sandia to reduce the cost of ownership (COO) of the deposition equipment by, for example, using a heated flow flange, which reduces the consumption of the expensive precursors (ammonia, nitrogen, hydrogen, and the metal organics) by 40 percent. Two different types of pyrometers developed by Sandia and Veeco are being tested by Philips Lumileds to control the substrate temperature, which helps determine the color of the LED. If all works according to plan, the COO will be reduced by at least 75 percent by improving throughput, growth uniformity, yield, and temperature stabilization.

  6. Method and apparatus for maximizing throughput of indirectly heated rotary kilns

    DOE Patents [OSTI]

    Coates, Ralph L; Smoot, L. Douglas; Hatfield, Kent E

    2012-10-30

    An apparatus and method for achieving improved throughput capacity of indirectly heated rotary kilns used to produce pyrolysis products such as shale oils or coal oils that are susceptible to decomposition by high kiln wall temperatures is disclosed. High throughput is achieved by firing the kiln such that optimum wall temperatures are maintained beginning at the point where the materials enter the heating section of the kiln and extending to the point where the materials leave the heated section. Multiple high velocity burners are arranged such that combustion products directly impact on the area of the kiln wall covered internally by the solid material being heated. Firing rates for the burners are controlled to maintain optimum wall temperatures.

  7. Omics Tools

    Energy Science and Technology Software Center (OSTI)

    2012-12-21

    The Omics Tools package provides several small trivial tools for work in genomics. This single portable package, the “omics.jar” file, is a toolbox that works in any Java-based environment, including PCs, Macs, and supercomputers. The number of tools is expected to grow. One tool (called cmsearch.hadoop or cmsearch.local), calls the external cmsearch program to predict non-coding RNA in a genome. The cmsearch program is part of the third-party Infernal package. Omics Tools does not containmore » Infernal. Infernal may be installed separately. The cmsearch.hadoop subtool requires Apache Hadoop and runs on a supercomputer, though cmsearch.local does not and runs on a server. Omics Tools does not contain Hadoop. Hadoop mat be installed separartely The other tools (cmgbk, cmgff, fastats, pal, randgrp, randgrpr, randsub) do not interface with third-party tools. Omics Tools is written in Java and Scala programming languages. Invoking the “help” command shows currently available tools, as shown below: schaumbe@gpint06:~/proj/omics$ java -jar omics.jar help Known commands are: cmgbk : compare cmsearch and GenBank Infernal hits cmgff : compare hits among two GFF (version 3) files cmsearch.hadoop : find Infernal hits in a genome, on your supercomputer cmsearch.local : find Infernal hits in a genome, on your workstation fastats : FASTA stats, e.g. # bases, GC content pal : stem-loop motif detection by palindromic sequence search (code stub) randgrp : random subsample without replacement, of groups randgrpr : random subsample with replacement, of groups (fast) randsub : random subsample without replacement, of file lines For more help regarding a particular command, use: java -jar omics.jar command help Usage: java -jar omics.jar command args« less

  8. Ensemble Jobs for Better Throughput | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ensemble Jobs for Better Throughput Start Date: Sep 28 2016 - 1:00pm to 3:30pm Event Website: http://www.alcf.anl.gov/workshops/ensemble-jobs-better-throughput Want better throughput for your small (<8K node) jobs? Ensemble jobs may hold the key. Ensemble jobs are ideal for users whose workloads include multiple small jobs (<8K nodes) that are suitable to run concurrently. During our interactive videoconference, you will: learn which job submission type is best for your specific workload

  9. Ensemble Jobs for Better Throughput | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ensemble Jobs for Better Throughput Ensemble Jobs for Better Throughput at the ALCF Sept. 28, 2016 | 1-3:30 p.m. central Want better throughput for your small (<8K node) jobs? Ensemble jobs may hold the key. Ensemble jobs are ideal for users whose workloads include multiple small jobs (<8K nodes) that are suitable to run concurrently. During our interactive videoconference, you will: learn which job submission type is best for your specific workload gain hands-on experience setting up an

  10. Research Tools

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Software & Tools Development Network R&D Software-Defined Networking (SDN) Experimental Network Testbeds Performance (perfSONAR) Software & Tools Development Data for Researchers Partnerships Publications Workshops Contact Us Technical Assistance: 1 800-33-ESnet (Inside US) 1 800-333-7638 (Inside US) 1 510-486-7600 (Globally) 1 510-486-7607 (Globally) Report Network Problems: trouble@es.net Provide Web Site Feedback: info@es.net Software & Tools Development Over the years, ESnet

  11. High-throughput liquid-absorption preconcentrator sampling methods

    DOE Patents [OSTI]

    Zaromb, S.

    1994-07-12

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis. 12 figs.

  12. Advanced method for high-throughput expression of mutated eukaryotic...

    Office of Scientific and Technical Information (OSTI)

    We explored the possibility of employing more than one PCR fragment to introduce various mutations in a single step, and found that when up to five PCR fragments were ...

  13. Vehicle Technologies Office Merit Review 2015: High-Throughput...

    Office of Environmental Management (EM)

    Presentation given by Ohio State University at 2015 DOE Hydrogen and Fuel Cells Program ... Modeling and Analysis Lean Gasoline System Development for Fuel Efficient Small Car

  14. Methods and devices for high-throughput dielectrophoretic concentration

    DOE Patents [OSTI]

    Simmons, Blake A.; Cummings, Eric B.; Fiechtner, Gregory J.; Fintschenko, Yolanda; McGraw, Gregory J.; Salmi, Allen

    2010-02-23

    Disclosed herein are methods and devices for assaying and concentrating analytes in a fluid sample using dielectrophoresis. As disclosed, the methods and devices utilize substrates having a plurality of pores through which analytes can be selectively prevented from passing, or inhibited, on application of an appropriate electric field waveform. The pores of the substrate produce nonuniform electric field having local extrema located near the pores. These nonuniform fields drive dielectrophoresis, which produces the inhibition. Arrangements of electrodes and porous substrates support continuous, bulk, multi-dimensional, and staged selective concentration.

  15. High throughput analysis of samples in flowing liquid

    DOE Patents [OSTI]

    Ambrose, W. Patrick; Grace, W. Kevin; Goodwin, Peter M.; Jett, James H.; Orden, Alan Van; Keller, Richard A.

    2001-01-01

    Apparatus and method enable imaging multiple fluorescent sample particles in a single flow channel. A flow channel defines a flow direction for samples in a flow stream and has a viewing plane perpendicular to the flow direction. A laser beam is formed as a ribbon having a width effective to cover the viewing plane. Imaging optics are arranged to view the viewing plane to form an image of the fluorescent sample particles in the flow stream, and a camera records the image formed by the imaging optics.

  16. High-throughput screening and device for photocatalysts

    DOE Patents [OSTI]

    Lewis, Nathan S.; Katz, Jordan; Gingrich, Todd

    2015-09-08

    The disclosure relates to compositions, devices and methods for screening of photocatalysts for water-splitting.

  17. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    three-dimensional shape. Although x-ray crystallography yields higher-resolution images, SAXS makes up for what it lacks in precision by providing fast, accurate...

  18. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    applying SAXS to focused biological problems. Current directions include the analysis of DNA repair pathways, which, if malfunctioning, are a leading cause of cancer. An equally...

  19. Towards Experimental Annotation of Genes by High Throughput Sequencing

    SciTech Connect (OSTI)

    Bradbury, Andrew

    2010-06-03

    Andrew Bradbury of Los Alamos National Laboratory discusses turning annotation into a sequencing pipeline on June 3, 2010 at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM

  20. Constant pressure high throughput membrane permeation testing system

    SciTech Connect (OSTI)

    Albenze, Erik J.; Hopkinson, David P.; Luebke, David R.

    2014-09-02

    The disclosure relates to a membrane testing system for individual evaluation of a plurality of planar membranes subjected to a feed gas on one side and a sweep gas on a second side. The membrane testing system provides a pressurized flow of a feed and sweep gas to each membrane testing cell in a plurality of membrane testing cells while a stream of retentate gas from each membrane testing cell is ported by a retentate multiport valve for sampling or venting, and a stream of permeate gas from each membrane testing cell is ported by a permeate multiport valve for sampling or venting. Back pressure regulators and mass flow controllers act to maintain substantially equivalent gas pressures and flow rates on each side of the planar membrane throughout a sampling cycle. A digital controller may be utilized to position the retentate and permeate multiport valves cyclically, allowing for gas sampling of different membrane cells over an extended period of time.

  1. High-Throughput and Combinatorial Screening of Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ternary system and find the cation matrix that stabilizes a certain anionic complex Theory guidance: Monte Carlo (MC) technique provides minimum energy structures for subsequent ...

  2. High Throughput Combinatorial Screening of Biometic Metal-Organic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cu 2+ Ni Pd Pt SBU metal ions bare metal Carboxylate linkers Vibrational Spectroscopy of Hydrogen Raman *H 2 in gas phase is Raman active due to large polarizability ...

  3. Compartmentalized microchannel array for high-throughput analysis...

    Office of Scientific and Technical Information (OSTI)

    is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth with applications ranging from bioenergy production to human health. ...

  4. Compartmentalized microchannel array for high-throughput analysis...

    Office of Scientific and Technical Information (OSTI)

    Authors: Geng, Tao 1 ; Bredeweg, Erin L. 1 ; Szymanski, Craig J. 1 ; Liu, Bingwen 1 ; Baker, Scott E. 1 ; Orr, Galya 1 ; Evans, James E. 1 ; Kelly, Ryan T. 1 + Show ...

  5. High-Throughput and Combinatorial Screening of Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Techniques in Hydrogen Storage Materials R&D Workshop Combinatorial Approaches for Hydrogen Storage Materials (presentation) FCTO Projects and the Materials Genome Initiative

  6. MRI contrast agents and high-throughput screening by MRI

    DOE Patents [OSTI]

    Lu, Yi; Yigit, Mehmet Veysel; Mazumdar, Debapriya

    2013-10-29

    The present invention provides an MRI contrast agent, comprising: MRI contrast agent particles, and oligonucleotides, attached to the particles.

  7. High-throughput liquid-absorption preconcentrator sampling methods

    DOE Patents [OSTI]

    Zaromb, Solomon (95706 William Dr., Hinsdale, IL 60521)

    1994-01-01

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis.

  8. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    is sufficient to address key biological questions. For example, future synthetic biology efforts may involve taking a useful protein, or a network of proteins, from one...

  9. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... * Frank Gayle * Mark Bailey * Xiongfel Shen Metal ...Combinatorial method(s) appropriate for the specific ... (DoDDLA new project) Philip Parilla * 11:40 Lunch ...

  10. High mass throughput particle generation using multiple nozzle spraying

    DOE Patents [OSTI]

    Pui, David Y.H.; Chen, Da-Ren

    2004-07-20

    Spraying apparatus and methods that employ multiple nozzle structures for producing multiple sprays of particles, e.g., nanoparticles, for various applications, e.g., pharmaceuticals, are provided. For example, an electrospray dispensing device may include a plurality of nozzle structures, wherein each nozzle structure is separated from adjacent nozzle structures by an internozzle distance. Sprays of particles are established from the nozzle structures by creating a nonuniform electrical field between the nozzle structures and an electrode electrically isolated therefrom.