National Library of Energy BETA

Sample records for high throughput pretreatment

  1. High Throughput Pretreatment and Enzyme Hydrolysis of Biomass: Screening Recalcitrance in Large Sample Populations (Presentation)

    SciTech Connect (OSTI)

    Decker, S. R.

    2010-10-01

    Presentation on the execution of the first high-throughput thermochemical pretreatment/enzyme digestion pipeline for screening biomass for recalcitrance.

  2. High throughput optical scanner

    DOE Patents [OSTI]

    Basiji, David A. (Seattle, WA); van den Engh, Gerrit J. (Seattle, WA)

    2001-01-01

    A scanning apparatus is provided to obtain automated, rapid and sensitive scanning of substrate fluorescence, optical density or phosphorescence. The scanner uses a constant path length optical train, which enables the combination of a moving beam for high speed scanning with phase-sensitive detection for noise reduction, comprising a light source, a scanning mirror to receive light from the light source and sweep it across a steering mirror, a steering mirror to receive light from the scanning mirror and reflect it to the substrate, whereby it is swept across the substrate along a scan arc, and a photodetector to receive emitted or scattered light from the substrate, wherein the optical path length from the light source to the photodetector is substantially constant throughout the sweep across the substrate. The optical train can further include a waveguide or mirror to collect emitted or scattered light from the substrate and direct it to the photodetector. For phase-sensitive detection the light source is intensity modulated and the detector is connected to phase-sensitive detection electronics. A scanner using a substrate translator is also provided. For two dimensional imaging the substrate is translated in one dimension while the scanning mirror scans the beam in a second dimension. For a high throughput scanner, stacks of substrates are loaded onto a conveyor belt from a tray feeder.

  3. High throughput protein production screening

    DOE Patents [OSTI]

    Beernink, Peter T. (Walnut Creek, CA); Coleman, Matthew A. (Oakland, CA); Segelke, Brent W. (San Ramon, CA)

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  4. High Throughput Materials Characterization John M. Gregoire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    White Paper for Establishing a User Facility for Synchrotron-based High Throughput Materials Characterization John M. Gregoire 1 , Matthew J. Kramer 2 , Apurva Mehta 3 1 Joint...

  5. ASYNCHRONOUS WAVE PIPELINES FOR HIGH THROUGHPUT DATAPATHS

    E-Print Network [OSTI]

    Huss, Sorin A.

    ASYNCHRONOUS WAVE PIPELINES FOR HIGH THROUGHPUT DATAPATHS O. Hauck and S. A. Huss fhauck j hussg pipeline architecture for high­speed clockless computation is proposed. It features gate­level pipelining the advantages of wave pipelining and asynchronous operation, i. e. very high throughput at low latency while

  6. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Robust, High-Throughput Analysis of Protein Structures Print Wednesday, 28 October 2009 00:00 Scientists have developed a...

  7. High-Throughput and Combinatorial Screening of Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput and Combinatorial Screening of Hydrogen Storage Materials (presentation) High-Throughput and Combinatorial Screening of Hydrogen Storage Materials (presentation)...

  8. Potential of High-Throughput Experimentation with Ammonia Borane...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of High-Throughput Experimentation with Ammonia Borane Solid Hydrogen Storage Materials (presentation) Potential of High-Throughput Experimentation with Ammonia Borane Solid...

  9. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D (presentation) High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D (presentation)...

  10. High Throughput/Combinatorial Screening of Hydrogen Storage Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Materials: UOP Approaches High ThroughputCombinatorial Screening of Hydrogen Storage Materials: UOP Approaches Presentation by Adriaan Sachtler from the High Throughput...

  11. High-throughput in vivo vertebrate screening

    E-Print Network [OSTI]

    Pardo-Martin, Carlos

    We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and ...

  12. Approaches to High Throughput Physical Organic Chemistry 

    E-Print Network [OSTI]

    Portal, Christophe

    2008-01-01

    Over the past ten years, the development of High Throughput (HT) synthetic chemistry techniques has allowed the rapid preparation of libraries of hundreds to thousands of compounds. These tools are now extensively used for drug and material...

  13. Chain Replication for Supporting High Throughput and Availability

    E-Print Network [OSTI]

    Keinan, Alon

    Chain Replication for Supporting High Throughput and Availability Robbert van Renesse rvr services that exhibit high throughput and availability with- out sacrificing strong consistency guarantees the perfor- mance characteristics of a prototype implementa- tion. Throughput, availability, and several

  14. The Design of High-Throughput Asynchronous Pipelines

    E-Print Network [OSTI]

    The Design of High-Throughput Asynchronous Pipelines Montek Singh Department of Computer Science UNIVERSITY December 2001 #12;The Design of High-Throughput Asynchronous Pipelines Montek Singh ABSTRACT is on high-throughput asynchronous pipelines, since pipelining is at the heart of most high

  15. The Design of HighThroughput Asynchronous Pipelines

    E-Print Network [OSTI]

    The Design of High­Throughput Asynchronous Pipelines Montek Singh Department of Computer Science UNIVERSITY December 2001 #12; The Design of High­Throughput Asynchronous Pipelines Montek Singh ABSTRACT is on high­throughput asynchronous pipelines, since pipelining is at the heart of most high

  16. Chemistry & Biology Genome-Wide High-Throughput Mining

    E-Print Network [OSTI]

    Yin, Jun

    Chemistry & Biology Article Genome-Wide High-Throughput Mining of Natural-Product Biosynthetic Gene.01.006 SUMMARY We have developed a phage-display method for high-throughput mining of bacterial gene clus- ters

  17. High-Throughput/Combinatorial Techniques in Hydrogen Storage...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D High-ThroughputCombinatorial Techniques in Hydrogen Storage Materials R&D On June 26, 2007 the Hydrogen...

  18. Statistical Algorithms for High-throughput Biological Data /

    E-Print Network [OSTI]

    Jeong, Kyowon

    2013-01-01

    Statistical Algorithms for High-throughput Biological Data by Kyowon Jeong Doctor of Philosophy in Electrical Engineering (Communication Theory

  19. Automated High Throughput Drug Target Crystallography

    SciTech Connect (OSTI)

    Rupp, B

    2005-02-18

    The molecular structures of drug target proteins and receptors form the basis for 'rational' or structure guided drug design. The majority of target structures are experimentally determined by protein X-ray crystallography, which as evolved into a highly automated, high throughput drug discovery and screening tool. Process automation has accelerated tasks from parallel protein expression, fully automated crystallization, and rapid data collection to highly efficient structure determination methods. A thoroughly designed automation technology platform supported by a powerful informatics infrastructure forms the basis for optimal workflow implementation and the data mining and analysis tools to generate new leads from experimental protein drug target structures.

  20. High throughput chemical munitions treatment system

    DOE Patents [OSTI]

    Haroldsen, Brent L. (Manteca, CA); Stofleth, Jerome H. (Albuquerque, NM); Didlake, Jr., John E. (Livermore, CA); Wu, Benjamin C-P (San Ramon, CA)

    2011-11-01

    A new High-Throughput Explosive Destruction System is disclosed. The new system is comprised of two side-by-side detonation containment vessels each comprising first and second halves that feed into a single agent treatment vessel. Both detonation containment vessels further comprise a surrounding ventilation facility. Moreover, the detonation containment vessels are designed to separate into two half-shells, wherein one shell can be moved axially away from the fixed, second half for ease of access and loading. The vessels are closed by means of a surrounding, clam-shell type locking seal mechanisms.

  1. High Throughput Combinatorial Screening of Biometic Metal-Organic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High Throughput Combinatorial Screening of Biometic Metal-Organic Materials for Military Hydrogen-Storage Materials (New Joint Miami UNREL DoDDLA Project) (presentation) High...

  2. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Robust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that...

  3. High-throughput metagenomic technologies for complex microbial...

    Office of Scientific and Technical Information (OSTI)

    formats Prev Next Title: High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats You are accessing a document from...

  4. High Throughput/Combinatorial Screening of Hydrogen Storage Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Materials (presentation) High ThroughputCombinatorial Screening of Hydrogen Storage Materials (presentation) Presented at the U.S. Department of Energy's Hydrogen Storage Meeting...

  5. High-Throughput Program for the Discovery of NOx Reduction Catalysts...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput Program for the Discovery of NOx Reduction Catalysts High-Throughput Program for the Discovery of NOx Reduction Catalysts 2004 Diesel Engine Emissions Reduction...

  6. Development and optimization of high-throughput zebrafish screening platform

    E-Print Network [OSTI]

    Koo, Bryan Kyo

    2010-01-01

    The high-throughput zebrafish screening platform is a revolutionary tool that enables subcellular precision in vivo whole animal screening of Danio Rerio. It can perform laser surgery and/or imaging in less than twenty ...

  7. High-throughput vertebrate total analysis/screening platform

    E-Print Network [OSTI]

    Chang, Tsung-Yao, Ph. D. Massachusetts Institute of Technology

    2012-01-01

    High-throughput screening (HTS) is seen as one of the most promising technologies to facilitate biomedical studies and pharmaceutical discoveries. Although large varieties of in vitro HTS technologies have opened great ...

  8. New Tools for High Throughput Chemistry and Biology 

    E-Print Network [OSTI]

    Galveia, Luciano Paulo Rosa

    2011-01-01

    New strategies for the high-throughput determination of protease substrate specificity using a dual colour microarray based format with a small library of FRET-based peptides were developed. Integral to this process was ...

  9. Pretreatment of high solid microbial sludges

    DOE Patents [OSTI]

    Rivard, C.J.; Nagle, N.J.

    1998-07-28

    A process and apparatus are disclosed for pretreating microbial sludges in order to enhance secondary anaerobic digestion. The pretreatment process involves disrupting the cellular integrity of municipal sewage sludge through a combination of thermal, explosive decompression and shear forces. The sludge is pressurized and pumped to a pretreatment reactor where it is mixed with steam to heat and soften the sludge. The pressure of the sludge is suddenly reduced and explosive decompression forces are imparted which partially disrupt the cellular integrity of the sludge. Shear forces are then applied to the sludge to further disrupt the cellular integrity of the sludge. Disrupting cellular integrity releases both soluble and insoluble organic constituents and thereby renders municipal sewage sludge more amenable to secondary anaerobic digestion. 1 fig.

  10. Pretreatment of high solid microbial sludges

    DOE Patents [OSTI]

    Rivard, Christopher J. (Lakewood, CO); Nagle, Nicholas J. (Broomfield, CO)

    1998-01-01

    A process and apparatus for pretreating microbial sludges in order to enhance secondary anaerobic digestion. The pretreatment process involves disrupting the cellular integrity of municipal sewage sludge through a combination of thermal, explosive decompression and shear forces. The sludge is pressurized and pumped to a pretreatment reactor where it is mixed with steam to heat and soften the sludge. The pressure of the sludge is suddenly reduced and explosive decompression forces are imparted which partially disrupt the cellular integrity of the sludge. Shear forces are then applied to the sludge to further disrupt the cellular integrity of the sludge. Disrupting cellular integrity releases both soluble and insoluble organic constituents and thereby renders municipal sewage sludge more amenable to secondary anaerobic digestion.

  11. Systematic reconstruction of RNA functional motifs with high throughput microfluidics

    E-Print Network [OSTI]

    Quake, Stephen R.

    Systematic reconstruction of RNA functional motifs with high throughput microfluidics Lance Martin1 Hill, NC 27599 Abstract We present RNA-MITOMI, a microfluidic platform for integrated synthesis microfluidic platform such that the entire RNA library could be simultaneously synthesized and then assayed

  12. High-Throughput Methodology for Discovery of Metal-Organic Frameworks...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    High-Throughput Methodology for Discovery of Metal-Organic Frameworks with a High Binding Energy (New Joint UC-BerkeleySymyx DoDDLA Project) (presentation) High-Throughput...

  13. High-ThroughputTagging of Arabidopsis Proteins

    E-Print Network [OSTI]

    Citovsky, Vitaly

    ­0124 (N.C., G.D., N.V.R.); and The Arabidopsis Information Resource (S.L., S.Y.R.) and Carnegie Institution of Washington (S.L., D.E., S.Y.R), Stanford, California 94305 We developed a high their chromogenic reac- tion products (Taylor, 1997). The use of antigenic tags, such as the T7 or HA epitopes

  14. Agenda from the U.S. Department of Energy's High Throughput Screening...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Agenda from the U.S. Department of Energy's High Throughput...

  15. Methods and technologies for high-throughput and high-content small animal screening

    E-Print Network [OSTI]

    Rohde, Christopher, 1979-

    2012-01-01

    High-throughput and high-content screening (HTS and HCS) of whole animals requires their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis ...

  16. High-throughput screening for modulators of cellular contractile force

    E-Print Network [OSTI]

    Park, Chan Young; Tambe, Dhananjay; Chen, Bohao; Lavoie, Tera; Dowell, Maria; Simeonov, Anton; Maloney, David J; Marinkovic, Aleksandar; Tschumperlin, Daniel J; Burger, Stephanie; Frykenberg, Matthew; Butler, James P; Stamer, W Daniel; Johnson, Mark; Solway, Julian; Fredberg, Jeffrey J; Krishnan, Ramaswamy

    2014-01-01

    When cellular contractile forces are central to pathophysiology, these forces comprise a logical target of therapy. Nevertheless, existing high-throughput screens are limited to upstream signaling intermediates with poorly defined relationship to such a physiological endpoint. Using cellular force as the target, here we screened libraries to identify novel drug candidates in the case of human airway smooth muscle cells in the context of asthma, and also in the case of Schlemm's canal endothelial cells in the context of glaucoma. This approach identified several drug candidates for both asthma and glaucoma. We attained rates of 1000 compounds per screening day, thus establishing a force-based cellular platform for high-throughput drug discovery.

  17. SAMDI Mass Spectrometry for High Throughput Discovery of Enzyme Function |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein StructuresMaintenance /

  18. Co-solvent Pretreatment Reduces Costly Enzyme Requirements for High Sugar and Ethanol Yields from

    E-Print Network [OSTI]

    California at Riverside, University of

    Co-solvent Pretreatment Reduces Costly Enzyme Requirements for High Sugar and Ethanol Yields from limits potential sugar yields.[7] Thus, it is desirable We introduce a new pretreatment called co-solvent

  19. Environmental characterization studies of a high-throughput wood gasifier

    SciTech Connect (OSTI)

    Chang, H.; Niemann, R.C.; Wilzbach, K.E.; Paisley, M.

    1983-01-01

    Potential environmental effects associated with thermochemical biomass gasification have been studied by Argonne National Laboratory in cooperation with Battelle Columbus Laboratories (BCL). A series of samples from the process research unit of an indirectly heated, high-throughput wood gasifier operated by BCL has been analyzed for potentially toxic organic compounds and trace elements. The results indicate that, under the test-run conditions, the gasification of both pine and hardwood is accompanied by the formation of some oil, the heavier fraction of which gives a positive response in the Ames assay for mutagenicity and contains numerous phenols and polycyclic aromatic hydrocarbons, including some carcinogens. The implications of these observations are discussed.

  20. High-throughput sequencing of cytosine methylation in plant DNA

    E-Print Network [OSTI]

    Hardcastle, Thomas J.

    2013-06-07

    . Genome Res 2008, 18(7):1051–1063. 66. Bormann Chung CA, Boyd VL, McKernan KJ, Fu Y, Monighetti C, Peckham HE, Barker M: Whole methylome analysis by ultra-deep sequencing using two-base encoding. PloS One 2010, 5(2):e9320. 67. Ondov BD, Cochran C, Landers... reads. Bioinformatics 2013, 29(2):268–270. 70. Minoche AE, Dohm JC, Himmelbauer H: Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and genome analyzer systems. Genome Biol 2011, 12(11):R112. 71. Wang XV, Blades N, Ding J...

  1. Computational Proteomics: High-throughput Analysis for Systems Biology

    SciTech Connect (OSTI)

    Cannon, William R.; Webb-Robertson, Bobbie-Jo M.

    2007-01-03

    High-throughput (HTP) proteomics is a rapidly developing field that offers the global profiling of proteins from a biological system. The HTP technological advances are fueling a revolution in biology, enabling analyses at the scales of entire systems (e.g., whole cells, tumors, or environmental communities). However, simply identifying the proteins in a cell is insufficient for understanding the underlying complexity and operating mechanisms of the overall system. Systems level investigations are relying more and more on computational analyses, especially in the field of proteomics generating large-scale global data.

  2. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast and

  3. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein Structures Print Scientists have developed a fast

  4. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-Throughput Analysis of Protein Structures Print Scientists have developed a

  5. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    E-Print Network [OSTI]

    Wu, Zhigang

    2009-01-01

    Soft inertial microfluidics for high throughput separation1 Introduction Microfluidics has gained significant advancesof mammalian cells using microfluidics 3,4 , there have been

  6. Automated MEMS-based Drosophila embryo injection system for high-throughput RNAi screens

    E-Print Network [OSTI]

    Scott, Matthew

    Automated MEMS-based Drosophila embryo injection system for high-throughput RNAi screens Stefan developed an automated system based on microelectromechanical systems (MEMS) injectors for reliable mass

  7. CONTINUOUS ROLL-TO-ROLL SERPENTINE DEPOSITION FOR HIGH THROUGHPUT a-Si PV MANUFACTURING

    E-Print Network [OSTI]

    Deng, Xunming

    CONTINUOUS ROLL-TO-ROLL SERPENTINE DEPOSITION FOR HIGH THROUGHPUT a-Si PV MANUFACTURING M. Izu, H. When this technique is incorporated into a continuous roll- to-roll PV manufacturing process a number of advantages in a fully automated high throughput PV module production plant [l-18

  8. Improving Bounds on the Football Pool Problem via Symmetry Reduction and High-Throughput Computing

    E-Print Network [OSTI]

    Linderoth, Jeffrey T.

    Improving Bounds on the Football Pool Problem via Symmetry Reduction and High-Throughput Computing of Wisconsin-Madison, 1210 W. Dayton St., Madison, WI 53706, USA, gthain@cs.wisc.edu The Football Pool Problem theory. Key words: Football Pool Problem; High-Throughput Computing; Branch-and-Bound; Con- dor; Master

  9. HPAZ: a High-throughput Pipeline Architecture of ZUC in Zongbin Liu1

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    HPAZ: a High-throughput Pipeline Architecture of ZUC in Hardware Zongbin Liu1 , Neng Gao1 , Jiwu@ist.psu.edu Abstract. In this paper, we propose a high-throughput pipeline architecture of the stream cipher ZUC which significantly reduces operating frequency. In this paper we design a mixed two-stage pipeline architecture which

  10. Assessing the Thermoelectric Properties of Sintered Compounds via High-Throughput Ab-Initio Calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    Assessing the Thermoelectric Properties of Sintered Compounds via High-Throughput Ab Database have been considered as nanograined, sintered-powder thermoelectrics with the high-throughput ab the electronic band gap and the carrier effective mass, and that the probability of having large thermoelectric

  11. Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived Substrates

    SciTech Connect (OSTI)

    Hamilton-Brehm, Scott; Vishnivetskaya, Tatiana A; Allman, Steve L; Mielenz, Jonathan R; Elkins, James G

    2012-01-01

    Flow cytometry (FCM) techniques have been developed for sorting mesophilic organisms, but the difficulty increases if the target microbes are thermophilic anaerobes. We demonstrate a reliable, high-throughput method of screening thermophilic anaerobic organisms using FCM and 96-well plates for growth on biomass-relevant substrates. The method was tested using the cellulolytic thermophiles Clostridium ther- mocellum (Topt = 55 C), Caldicellulosiruptor obsidiansis (Topt = 78 C) and the fermentative hyperthermo- philes, Pyrococcus furiosus (Topt = 100 C) and Thermotoga maritima (Topt = 80 C). Multi-well plates were incubated at various temperatures for approximately 72 120 h and then tested for growth. Positive growth resulting from single cells sorted into individual wells containing an anaerobic medium was verified by OD600. Depending on the growth substrate, up to 80 % of the wells contained viable cultures, which could be transferred to fresh media. This method was used to isolate thermophilic microbes from Rabbit Creek, Yellowstone National Park (YNP), Wyoming. Substrates for enrichment cultures including crystalline cellulose (Avicel), xylan (from Birchwood), pretreated switchgrass and Populus were used to cultivate organisms that may be of interest to lignocellulosic biofuel production.

  12. Solion ion source for high-efficiency, high-throughput solar cell manufacturing

    SciTech Connect (OSTI)

    Koo, John Binns, Brant; Miller, Timothy; Krause, Stephen; Skinner, Wesley; Mullin, James

    2014-02-15

    In this paper, we introduce the Solion ion source for high-throughput solar cell doping. As the source power is increased to enable higher throughput, negative effects degrade the lifetime of the plasma chamber and the extraction electrodes. In order to improve efficiency, we have explored a wide range of electron energies and determined the conditions which best suit production. To extend the lifetime of the source we have developed an in situ cleaning method using only existing hardware. With these combinations, source life-times of >200 h for phosphorous and >100 h for boron ion beams have been achieved while maintaining 1100 cell-per-hour production.

  13. Opal: A Multi-radio Platform for High Throughput Wireless Sensor Networks

    E-Print Network [OSTI]

    Langendoen, Koen

    1 Opal: A Multi-radio Platform for High Throughput Wireless Sensor Networks Raja Jurdak, Kevin requirements. This letter presents the design of the versatile Opal platform that couples a Cortex M3 MCU sacrificing communication range. We present experiments that evaluate Opal's throughput and range when

  14. Low inlet gas velocity high throughput biomass gasifier

    SciTech Connect (OSTI)

    Feldmann, Herman F.; Paisley, Mark A.

    1989-01-01

    The present invention discloses a novel method of operating a gasifier for production of fuel gas from carbonaceous fuels. The process disclosed enables operating in an entrained mode using inlet gas velocities of less than 7 feet per second, feedstock throughputs exceeding 4000 lbs/ft.sup.2 -hr, and pressures below 100 psia.

  15. Evaluation of High Solids Alkaline Pretreatment of Rice Straw

    E-Print Network [OSTI]

    Cheng, Yu-Shen; Zheng, Yi; Yu, Chao Wei; Dooley, Todd M.; Jenkins, Bryan M.; VanderGheynst, Jean S.

    2010-01-01

    19. Oates, J. A. H. (2007). Lime and Limestone 207–211. 20.1–28. 41. Kim, S. H. (2005). Lime pretreatment and enzymaticunwashed biomass. Keywords Lime . Calcium hydroxide . Sodium

  16. The Materials genome : rapid materials screening for renewable energy using high-throughput density functional theory

    E-Print Network [OSTI]

    Jain, Anubhav, Ph.D. Massachusetts Institute of Technology

    2011-01-01

    This thesis relates to the emerging field of high-throughput density functional theory (DFT) computation for materials design and optimization. Although highthroughput DFT is a promising new method for materials discovery, ...

  17. Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing

    E-Print Network [OSTI]

    Larman, Harry Benjamin

    2012-01-01

    High throughput methods in molecular biology have changed the landscape of biomedical research. In particular, advances in massively parallel DNA sequencing and synthesis technologies are defining our genomes and the ...

  18. CellVisualizer : exploring hierarchical, multi-dimensional data with applications to high-throughput microscopy

    E-Print Network [OSTI]

    Kang, InHan

    2006-01-01

    In this thesis, we present a system for visualizing hierarchical, multi-dimensional, memory-intensive datasets. Specifically, we designed an interactive system to visualize data collected by high-throughput microscopy and ...

  19. Cell bioprinting as a potential high-throughput method for fabricating cell-based biosensors (CBBs)

    E-Print Network [OSTI]

    Xu, F.

    Cell-based biosensors (CBBs) are becoming an important tool for biosecurity applications and rapid diagnostics. For current CBBs technology, cell immobilization and high throughput fabrication are the main challenges. To ...

  20. Infrastructure to support ultra high throughput biodosimetry screening after a radiological event

    E-Print Network [OSTI]

    Brenner, David Jonathan

    GUY GARTY1 , ANDREW KARAM2 , & DAVID J. BRENNER3 1 Radiological Research Accelerator Facility, Radiological Research Accelerator Facility, Nevis Laboratories, Columbia UniverInfrastructure to support ultra high throughput biodosimetry screening after a radiological event

  1. Engineering a single cell microarray platform for high throughput DNA damage and repair analysis

    E-Print Network [OSTI]

    Weingeist, David McGregor

    2012-01-01

    DNA damage contributes to cancer, aging, and heritable diseases. Ironically, DNA damaging agents are also commonly used in current cancer treatment. We therefore need robust, high throughput, and inexpensive tools for ...

  2. An instrument for high-throughput measurements of fiber mechanical properties

    E-Print Network [OSTI]

    Kristofek, Grant William, 1980-

    2005-01-01

    In this thesis, an instrument is designed and constructed for the purpose of measuring the mechanical properties of single fibers. The instrument is intended to provide high throughput measurement of single fiber geometric ...

  3. Stochastic Forecasts Achieve High Throughput and Low Delay over Cellular Networks

    E-Print Network [OSTI]

    Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Balakrishnan, Hari; Winstein, Keith J.; Sivaraman Kaushalram, Anirudh; Balakrishnan, Hari

    Sprout is an end-to-end transport protocol for interactive applications that desire high throughput and low delay. Sprout works well over cellular wireless networks, where link speeds change dramatically with time, and ...

  4. Software for supporting large scale data processing for High Throughput Screening

    E-Print Network [OSTI]

    Tai, David

    2011-12-31

    High Throughput Screening for is a valuable data generation technique for data driven knowledge discovery. Because the rate of data generation is so great, it is a challenge to cope with the demands of post experiment data analysis. This thesis...

  5. Inertio-elastic focusing of bioparticles in microchannels at high throughput

    E-Print Network [OSTI]

    Lim, Eugene J.

    Controlled manipulation of particles from very large volumes of fluid at high throughput is critical for many biomedical, environmental and industrial applications. One promising approach is to use microfluidic technologies ...

  6. A microfluidic device for high throughput bacterial biofilm studies Jeongyun Kim,a

    E-Print Network [OSTI]

    Wood, Thomas K.

    A microfluidic device for high throughput bacterial biofilm studies Jeongyun Kim,a Manjunath Hegde of biofilm community formation. Here, we describe the development of a PDMS-based two-layer microfluidic flow

  7. SAMNet: a network-based approach to integrate multi-dimensional high throughput datasets

    E-Print Network [OSTI]

    Spencer, Sarah J.

    The rapid development of high throughput biotechnologies has led to an onslaught of data describing genetic perturbations and changes in mRNA and protein levels in the cell. Because each assay provides a one-dimensional ...

  8. Attendees list from the U.S. Department of Energy's High Throughput...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Attendees list from the U.S. Department of Energy's High Throughput Screening of Hydrogen Storage Materials Workshop on June 26, 2007 Attendees list from the U.S. Department of...

  9. A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial arrays

    E-Print Network [OSTI]

    Sun, Yu

    A microfabricated platform for high-throughput unconfined compression of micropatterned biomaterial8, Canada b Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College 16 September 2009 Available online 9 October 2009 Keywords: Hydrogel Micropatterning Biomaterial

  10. First principles high throughput screening of oxynitrides for water-splitting photocatalysts

    E-Print Network [OSTI]

    Wu, Yabi

    2013-01-01

    In this paper, we present a first principles high throughput screening system to search for new water-splitting photocatalysts. We use the approach to screen through nitrides and oxynitrides. Most of the known photocatalytic ...

  11. Development of High-Throughput Microfluidic Impedance Spectroscopy Platform for Analyzing Microdroplets in Droplet Microfluidic System 

    E-Print Network [OSTI]

    Sobahi, Nebras MohammedKamal A.

    2014-07-22

    This thesis presents the development of a high-throughput microfluidic impedance spectroscopy platform for electrically detecting analyzing impedance measurements of non-contact and label free microdroplets. This microfluidic impedance spectroscopy...

  12. Optical Multiplexing for High-Throughput Spectroscopic Analysis. 

    E-Print Network [OSTI]

    Ahmed, Saadiah

    2012-05-03

    in optical biosensor testing as it provides greater throughput and scalability while being an economical and compact solution. v NOMENCLATURE MFPF Multi Frequency Phase Fluorometer MUX Multiplexer LED Light Emitting Diode DIO Digital Input... oxidase in hybrid silicate microsphere that is coated with nanofilm and reference dye (RITC) (Fig. 1) [2]. The phosphorescent dye is excited by a green light from a LED (light emitting diode) to emit luminescence, the intensity of which is dependent...

  13. PARALLEL HIGH THROUGHPUT SOFT-OUTPUT SPHERE DECODER Q. Qi, C. Chakrabarti

    E-Print Network [OSTI]

    Kambhampati, Subbarao

    - putation complexity of only the list generator. Recently, a high speed systolic-like soft-output spherePARALLEL HIGH THROUGHPUT SOFT-OUTPUT SPHERE DECODER Q. Qi, C. Chakrabarti School of Electrical,chaitali}@asu.edu ABSTRACT Multiple-Input-Multiple-Output communication systems de- mand fast sphere decoding with high

  14. Low inlet gas velocity high throughput biomass gasifier

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.

    1989-05-09

    A method is described for operating a gasifier which comprises: introducing inlet gas at a velocity of about 0.5 to 7 ft/sec to fluidize a bed in a gasifier vessel; forming the bed into a fluidized bed in a first space region by means of the inlet gas, the fluidized bed containing a circulating hot relatively fine and inert solid bed particle component; inputting and throughputting carbonaceous material into and through the first space region with fluidized bed at a rate from 500-4400 lbs/ft/sup 2/-hr; endothermally pyrolyzing the carbonaceous material by means of the circulating hot inert particle component so as to form a product gas; forming contiguous to and above the fluidized bed a lower average density entrained space region containing an entrained mixture of inert solid particles, char, and carbonaceous material and the product gas; gradually and continuously removing the entrained mixture and the product gas from the lower average density entrained space region of the gasifier to a separator, residence time of the carbonaceous material in the gasifier not exceeding 3 minutes on average; separating the entrained mixture from the product gas; passing the entrained mixture containing inert solid particles, char, and carbonaceous material through an exothermic reaction zone to add heat; and returning at least the inert solid particles to the first space region.

  15. Droplet microfluidic technology for single-cell high-throughput screening

    E-Print Network [OSTI]

    Perrimon, Norbert

    Droplet microfluidic technology for single-cell high-throughput screening Eric Brouzesa,b,1 (received for review March 31, 2009) We present a droplet-based microfluidic technology that enables high our droplet microfluidic platform is modular, robust, uses no moving parts, and has a wide range

  16. Optical microplates for high-throughput screening of photosynthesis in lipid-producing algae{,

    E-Print Network [OSTI]

    Basu, Amar S.

    Optical microplates for high-throughput screening of photosynthesis in lipid- producing algae-producing algae of interest in 2nd generation biofuels. By conducting 96 experiments in parallel, photoirradiance the study of photosynthesis in algae. Societal challenges in energy sustainability have renewed interest

  17. UFlood: High-Throughput Flooding over Wireless Mesh Networks Jayashree Subramanian, Robert Morris, and Hari Balakrishnan

    E-Print Network [OSTI]

    UFlood: High-Throughput Flooding over Wireless Mesh Networks Jayashree Subramanian, Robert Morris--This paper proposes UFlood, a flooding protocol for wireless mesh networks. UFlood targets situations of completion of a flood to the slowest receiving node and total time spent transmitting. The key to achieving

  18. User, Robot and Automation Evaluations in High-Throughput Biological Screening Processes

    E-Print Network [OSTI]

    Kaber, David B.

    User, Robot and Automation Evaluations in High-Throughput Biological Screening Processes Noa Segall Cognitive Ergonomics Laboratory North Carolina State University Raleigh, NC +1 (919) 513-4494 nsegall@ncsu.edu Rebecca S. Green Cognitive Ergonomics Laboratory North Carolina State University Raleigh, NC +1 (919) 513

  19. Achieving High Throughput Ground-to-UAV Transport via Parallel Links

    E-Print Network [OSTI]

    Kung, H. T.

    Achieving High Throughput Ground-to-UAV Transport via Parallel Links Chit-Kwan Lin, H. T. Kung in unmanned aerial vehicle (UAV) applications, is a challenge due to varying channel quality and extended link diversity; 802.11; link layer; transport layer; UAV; network coding I. INTRODUCTION In this paper, we focus

  20. Droplet microfluidic technology for single-cell high-throughput screening

    E-Print Network [OSTI]

    Higgins, Darren

    , Lexington, MA 02421; and bGenetics Department, Harvard Medical School and cHoward Hughes Medical Institute oil and enables the digital manipulation of these reactors at a very high-throughput. Here, we by an immiscible oil. The advantages of this droplet- based technique include the physical and chemical isolation

  1. Comparison of High-Throughput Electrochemical Methods for Testing Direct Methanol Fuel Cell Anode Electrocatalysts

    E-Print Network [OSTI]

    power applications, fuel cells must compete with commodity power sources such as fossil fuels power sources, and mobile/portable applications, fuel cells that utilize liquid fuels can representComparison of High-Throughput Electrochemical Methods for Testing Direct Methanol Fuel Cell Anode

  2. Knife-Edge Scanning Microscopy: High-throughput Imaging and Analysis of

    E-Print Network [OSTI]

    Choe, Yoonsuck

    : High-throughput Imaging and Analysis of Massive Volumes of Biological Microstructures Author 1 Yoonsuck Choe Author 1 - Address Department of Computer Science, Texas A&M Univ. 3112 TAMU, College Station, TX, 77843-3112, USA Author 1 - Email choe@tamu.edu Author 1 - Tel +1 979 845 5466 Author 2 Louise C. Abbott

  3. Grid Portal Interface for Interactive Use and Monitoring of High-Throughput Proteome

    E-Print Network [OSTI]

    Bourne, Philip E.

    Grid Portal Interface for Interactive Use and Monitoring of High-Throughput Proteome Annotation, #12;54 A. Shahab et al. portal solution for grid computing in life sciences under the auspices, and the use of a GridSpeed [6] generated iGAP application portal are described. The portal solution was part

  4. S-box pipelining using genetic algorithms for high-throughput AES implementations: How

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    S-box pipelining using genetic algorithms for high-throughput AES implementations: How fast can we. In this manuscript, we explore how Genetic Algorithms (GAs) can be used for pipelining the AES substitution box based the implementation area [5,6]. However, when considering fast hardware implementations, pipelining is an obvious

  5. TECHNICAL ADVANCE A high-throughput method for quantifying growth of

    E-Print Network [OSTI]

    Dangl, Jeff

    : Arabidopsis, resistance genes, Pseudomonas syringae, phytopathogenic bacteria, counting bacteria IntroductionTECHNICAL ADVANCE A high-throughput method for quantifying growth of phytopathogenic bacteria; email: dangl@email.unc.edu) Summary Measuring the growth of pathogenic bacteria in leaves is a mainstay

  6. ORIGINAL ARTICLE GeoChip 3.0 as a high-throughput tool for analyzing

    E-Print Network [OSTI]

    Thomas, David D.

    significantly changed with the plant species diversity. As expected, GeoChip 3.0 is a high-throughput powerful Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA and 8 Virtual Institute in microbial ecology Keywords: microarray; functional genes; microbial community; plant diversity Introduction

  7. ORIGINAL ARTICLE GeoChip 3.0 as a high-throughput tool for analyzing

    E-Print Network [OSTI]

    Hazen, Terry

    significantly changed with the plant species diversity. As expected, GeoChip 3.0 is a high-throughput powerful Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA and 8 Virtual Institute Keywords: microarray; functional genes; microbial community; plant diversity Introduction Microorganisms

  8. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Hantke, Max, F.

    Preprocessed detector images that were used for the paper "High-throughput imaging of heterogeneous cell organelles with an X-ray laser". The CXI file contains the entire recorded data - including both hits and blanks. It also includes down-sampled images and LCLS machine parameters. Additionally, the Cheetah configuration file is attached that was used to create the pre-processed data.

  9. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Hantke, Max, F.

    2014-11-17

    Preprocessed detector images that were used for the paper "High-throughput imaging of heterogeneous cell organelles with an X-ray laser". The CXI file contains the entire recorded data - including both hits and blanks. It also includes down-sampled images and LCLS machine parameters. Additionally, the Cheetah configuration file is attached that was used to create the pre-processed data.

  10. Journal of Power Sources 158 (2006) 6068 High throughput evaluation of perovskite-based anode catalysts

    E-Print Network [OSTI]

    Mukasyan, Alexander

    2006-01-01

    Journal of Power Sources 158 (2006) 60­68 High throughput evaluation of perovskite-based anode to 25 compositions simultaneously under DMFC conditions. It was found that the Sr-based perovskites.g. perovskites, cuprates, etc.) Corresponding author. Tel.: +1 765 494 4075; fax: +1 765 494 0805. E

  11. Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical

    E-Print Network [OSTI]

    Zhu, Xiangdong

    Oblique-incidence reflectivity difference microscope for label-free high-throughput detection (OI-RD) microscope, a form of polarization-modulated imaging ellipsometer, for label on the polarizer­ compensator­sample­analyzer scheme and under the off-null condition, a polarization-modulated OI

  12. The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural

    E-Print Network [OSTI]

    Hendrickson, Wayne A.

    The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform Springer Science+Business Media B.V. 2010 Abstract The New York Consortium on Membrane Protein Structure Core Laboratory, New York Structural Biology Center, New York, NY 10027, USA F. Mancia Á M. Zhou

  13. nAture methods | VOL.12 NO.1 | JANUARY2015 | 71 high-throughput methods based on chromosome conformation

    E-Print Network [OSTI]

    Cai, Long

    that of hi-c. coupling this method with dnA-capture technology provides a high-throughput approachArticles nAture methods | VOL.12 NO.1 | JANUARY2015 | 71 high-throughput methods based for targeted mapping of fine-scale chromatin architecture. We applied targeted dnase hi-c to characterize the 3

  14. MEMORY SUB-BANKING SCHEME FOR HIGH THROUGHPUT TURBO DECODER Mayank Tiwari, Yuming Zhu and Chaitali Chakrabarti

    E-Print Network [OSTI]

    Kambhampati, Subbarao

    level are needed in order to design low-power high- throughput Turbo decoders for mobile systems, the sliding win- dow (SW) approach has been proposed as an effective means of reduc- ing the decoding delay-banked implemen- tation of the SW-based approach that achieves high throughput, low decoding latency and reduced

  15. High-throughput metagenomic technologies for complex microbial...

    Office of Scientific and Technical Information (OSTI)

    within the context of environmental applications and focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference...

  16. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    described here, which the research team believes will help usher in the next phase of genomics research. The high flux, brilliance, and focus of the x rays from ALS Beamline 12.3.1...

  17. Automated High Throughput Protein Crystallization Screening at Nanoliter Scale and Protein Structural Study on Lactate Dehydrogenase

    SciTech Connect (OSTI)

    Fenglei Li

    2006-08-09

    The purposes of our research were: (1) To develop an economical, easy to use, automated, high throughput system for large scale protein crystallization screening. (2) To develop a new protein crystallization method with high screening efficiency, low protein consumption and complete compatibility with high throughput screening system. (3) To determine the structure of lactate dehydrogenase complexed with NADH by x-ray protein crystallography to study its inherent structural properties. Firstly, we demonstrated large scale protein crystallization screening can be performed in a high throughput manner with low cost, easy operation. The overall system integrates liquid dispensing, crystallization and detection and serves as a whole solution to protein crystallization screening. The system can dispense protein and multiple different precipitants in nanoliter scale and in parallel. A new detection scheme, native fluorescence, has been developed in this system to form a two-detector system with a visible light detector for detecting protein crystallization screening results. This detection scheme has capability of eliminating common false positives by distinguishing protein crystals from inorganic crystals in a high throughput and non-destructive manner. The entire system from liquid dispensing, crystallization to crystal detection is essentially parallel, high throughput and compatible with automation. The system was successfully demonstrated by lysozyme crystallization screening. Secondly, we developed a new crystallization method with high screening efficiency, low protein consumption and compatibility with automation and high throughput. In this crystallization method, a gas permeable membrane is employed to achieve the gentle evaporation required by protein crystallization. Protein consumption is significantly reduced to nanoliter scale for each condition and thus permits exploring more conditions in a phase diagram for given amount of protein. In addition, evaporation rate can be controlled or adjusted in this method during the crystallization process to favor either nucleation or growing processes for optimizing crystallization process. The protein crystals gotten by this method were experimentally proven to possess high x-ray diffraction qualities. Finally, we crystallized human lactate dehydrogenase 1 (H4) complexed with NADH and determined its structure by x-ray crystallography. The structure of LDH/NADH displays a significantly different structural feature, compared with LDH/NADH/inhibitor ternary complex structure, that subunits in LDH/NADH complex show open conformation or two conformations on the active site while the subunits in LDH/NADH/inhibitor are all in close conformation. Multiple LDH/NADH crystals were obtained and used for x-ray diffraction experiments. Difference in subunit conformation was observed among the structures independently solved from multiple individual LDH/NADH crystals. Structural differences observed among crystals suggest the existence of multiple conformers in solution.

  18. High-Throughput Bioscience Center (HTBC) History and Mission

    E-Print Network [OSTI]

    Kay, Mark A.

    pipettor (5-200 uL) Primary used for hit picking #12;SciClone ALH3000 Low Volume Head Can exchange heads3000 SciClone ALH3000, featuring... High Volume Head 96 disposal-tip pipettor (5-200 uL) Can change for 96 to 96 plate replication and 96 to 384 plate reformatting Z8 Head 8 independent disposable tip

  19. High Throughput/Combinatorial Screening of Hydrogen Storage Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancing ProgramsDepartment of¡High

  20. High Throughput/Combinatorial Screening of Hydrogen Storage Materials: UOP

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancing ProgramsDepartment of¡HighApproaches | Department of

  1. High-Throughput and Combinatorial Screening of Hydrogen Storage Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancingR Walls - Building America Topa High Binding Energy

  2. High-Throughput Analytical Model to Evaluate Materials for Temperature

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid you notHeat Pumps Heat Pumps An error occurred. Try|High-Powered

  3. High-throughput metagenomic technologies for complex microbial community

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article)lasers (Journal Article)SciTechHigh-contrastproperties ofwallanalysis.

  4. High-throughput metagenomic technologies for complex microbial community

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article)lasers (Journal Article)SciTechHigh-contrastproperties

  5. High Throughput Combinatorial Screening of Biometic Metal-Organic Materials

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancing ProgramsDepartment of¡High HIGHofWasteDepartmentfor

  6. High-throughput metagenomic technologies for complex microbial community analysis. Open and closed formats

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhou, Jizhong; He, Zhili; Yang, Yunfeng; Deng, Ye; Tringe, Susannah G.; Alvarez-Cohen, Lisa

    2015-01-27

    Understanding the structure, functions, activities and dynamics of microbial communities in natural environments is one of the grand challenges of 21st century science. To address this challenge, over the past decade, numerous technologies have been developed for interrogating microbial communities, of which some are amenable to exploratory work (e.g., high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e.g., phylogenetic and functional gene arrays). Here, we provide a critical review and synthesis of the most commonly applied “open-format” and “closed-format” detection technologies. We discuss their characteristics, advantages, and disadvantages within the context of environmental applications andmore »focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference genomes are few. In addition, we discuss crucial issues and considerations associated with applying complementary high-throughput molecular technologies to address important ecological questions.« less

  7. Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

    SciTech Connect (OSTI)

    Hura, Greg L.; Menon, Angeli L.; Hammel, Michal; Rambo, Robert P.; Poole II, Farris L.; Tsutakawa, Susan E.; Jenney Jr, Francis E.; Classen, Scott; Frankel, Kenneth A.; Hopkins, Robert C.; Yang, Sungjae; Scott, Joseph W.; Dillard, Bret D.; Adams, Michael W. W.; Tainer, John A.

    2009-07-20

    We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.

  8. A 96-well microplate incorporating a replica molded microfluidic network integrated with photonic crystal biosensors for high throughput kinetic

    E-Print Network [OSTI]

    Cunningham, Brian

    crystal biosensors for high throughput kinetic biomolecular interaction analysis{; Charles J. Choi microfluidic channels, with integrated label-free photonic crystal biosensors as the bottom surface the volume of reagent required to functionalize the biosensor surface, while retaining compatibility

  9. Evaluation of Tavorite-Structured Cathode Materials for Lithium-Ion Batteries Using High-Throughput Computing

    E-Print Network [OSTI]

    Mueller, Tim

    Cathode materials with structure similar to the mineral tavorite have shown promise for use in lithium-ion batteries, but this class of materials is relatively unexplored. We use high-throughput density-functional-theory ...

  10. High-throughput data mined prediction of inorganic compounds and computational discovery of new lithium-ion battery cathode materials

    E-Print Network [OSTI]

    Hautier, Geoffroy (Geoffroy T. F.)

    2011-01-01

    The ability to computationally predict the properties of new materials, even prior to their synthesis, has been made possible due to the current accuracy of modern ab initio techniques. In some cases, high-throughput ...

  11. High Throughput Plasmid Sequencing with Illumina and CLC Bio (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema (OSTI)

    Athavale, Ajay [Monsanto

    2013-01-25

    Ajay Athavale (Monsanto) presents "High Throughput Plasmid Sequencing with Illumina and CLC Bio" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  12. Advances in high-throughput speed, low-latency communication for embedded instrumentation ( 7th Annual SFAF Meeting, 2012)

    ScienceCinema (OSTI)

    Jordan, Scott [Physik Instrumente

    2013-02-11

    Scott Jordan on "Advances in high-throughput speed, low-latency communication for embedded instrumentation" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  13. High Throughput Computing Impact on Meta Genomics (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    SciTech Connect (OSTI)

    Gore, Brooklin [Morgridge Institute for Research] [Morgridge Institute for Research

    2011-10-12

    This presentation includes a brief background on High Throughput Computing, correlating gene transcription factors, optical mapping, genotype to phenotype mapping via QTL analysis, and current work on next gen sequencing.

  14. High Throughput Computing Impact on Meta Genomics (Metagenomics Informatics Challenges Workshop: 10K Genomes at a Time)

    ScienceCinema (OSTI)

    Gore, Brooklin [Morgridge Institute for Research

    2013-01-22

    This presentation includes a brief background on High Throughput Computing, correlating gene transcription factors, optical mapping, genotype to phenotype mapping via QTL analysis, and current work on next gen sequencing.

  15. High Throughput Plasmid Sequencing with Illumina and CLC Bio (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    SciTech Connect (OSTI)

    Athavale, Ajay [Monsanto] [Monsanto

    2012-06-01

    Ajay Athavale (Monsanto) presents "High Throughput Plasmid Sequencing with Illumina and CLC Bio" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  16. High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins

    DOE Patents [OSTI]

    Kim, Sung-Hou (Moraga, CA); Kim, Rosalind (Moraga, CA); Jancarik, Jamila (Walnut Creek, CA)

    2012-01-31

    An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.

  17. Development of a high-throughput microfluidic integrated microarray for the detection of chimeric bioweapons.

    SciTech Connect (OSTI)

    Sheppod, Timothy; Satterfield, Brent; Hukari, Kyle W.; West, Jason A. A.; Hux, Gary A.

    2006-10-01

    The advancement of DNA cloning has significantly augmented the potential threat of a focused bioweapon assault, such as a terrorist attack. With current DNA cloning techniques, toxin genes from the most dangerous (but environmentally labile) bacterial or viral organism can now be selected and inserted into robust organism to produce an infinite number of deadly chimeric bioweapons. In order to neutralize such a threat, accurate detection of the expressed toxin genes, rather than classification on strain or genealogical decent of these organisms, is critical. The development of a high-throughput microarray approach will enable the detection of unknowns chimeric bioweapons. The development of a high-throughput microarray approach will enable the detection of unknown bioweapons. We have developed a unique microfluidic approach to capture and concentrate these threat genes (mRNA's) upto a 30 fold concentration. These captured oligonucleotides can then be used to synthesize in situ oligonucleotide copies (cDNA probes) of the captured genes. An integrated microfluidic architecture will enable us to control flows of reagents, perform clean-up steps and finally elute nanoliter volumes of synthesized oligonucleotides probes. The integrated approach has enabled a process where chimeric or conventional bioweapons can rapidly be identified based on their toxic function, rather than being restricted to information that may not identify the critical nature of the threat.

  18. Enabling a High Throughput Real Time Data Pipeline for a Large Radio Telescope Array with GPUs

    E-Print Network [OSTI]

    Edgar, R G; Dale, K; Mitchell, D A; Ord, S M; Wayth, R B; Pfister, H; Greenhill, L J

    2010-01-01

    The Murchison Widefield Array (MWA) is a next-generation radio telescope currently under construction in the remote Western Australia Outback. Raw data will be generated continuously at 5GiB/sec. This high throughput motivates the development of on-site, real time processing and reduction in preference to archiving, transport and off-line processing. Maintaining real time operation will require a sustained performance of around 2.5TFLOP/sec (including convolutions, FFTs, interpolations and matrix multiplications). We describe a scalable heterogeneous computing pipeline implementation, exploiting both the high computing density and FLOP-per-Watt ratio of modern GPUs. The architecture is highly parallel within and across nodes, with all major processing elements performed by GPUs. Necessary scatter-gather operations along the pipeline are loosely synchronized and implemented in MPI. The MWA will be a frontier scientific instrument and a pathfinder for planned peta- and exascale facilities.

  19. Heterogeneous High Throughput Scientific Computing with APM X-Gene and Intel Xeon Phi

    E-Print Network [OSTI]

    David Abdurachmanov; Brian Bockelman; Peter Elmer; Giulio Eulisse; Robert Knight; Shahzad Muzaffar

    2014-10-10

    Electrical power requirements will be a constraint on the future growth of Distributed High Throughput Computing (DHTC) as used by High Energy Physics. Performance-per-watt is a critical metric for the evaluation of computer architectures for cost- efficient computing. Additionally, future performance growth will come from heterogeneous, many-core, and high computing density platforms with specialized processors. In this paper, we examine the Intel Xeon Phi Many Integrated Cores (MIC) co-processor and Applied Micro X-Gene ARMv8 64-bit low-power server system-on-a-chip (SoC) solutions for scientific computing applications. We report our experience on software porting, performance and energy efficiency and evaluate the potential for use of such technologies in the context of distributed computing systems such as the Worldwide LHC Computing Grid (WLCG).

  20. High-Throughput, High-Precision Hot Testing Tool for High-Brightness

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing Tool Fits the Bill Financing ToolSustainableSecurityHighDepartment ofLight-Emitting Diode

  1. In-tank pretreatment of high-level tank wastes: The SIPS system

    SciTech Connect (OSTI)

    Reich, M.; Powell, J.; Barletta, R.

    1996-03-01

    A new approach, termed SIPS (Small In-Tank Processing System), that enables the in-tank processing and separation of high-level tank wastes into high-level waste (HLW) and low-level waste (LLW) streams that are suitable for vitrification, is described. Presently proposed pretreatment systems, such as enhanced sludge washing (ESW) and TRUEX, require that the high-level tank wastes be retrieved and pumped to a large, centralized processing facility, where the various waste components are separated into a relatively small, radioactively concentrated stream (HLW), and a relatively large, predominantly non-radioactive stream (LLW). In SIPS, a small process module, typically on the order of 1 meter in diameter and 4 meters in length, is inserted into a tank. During a period of approximately six months, it processes the solid/liquid materials in the tank, separating them into liquid HLW and liquid LLW output streams that are pumped away in two small diameter (typically 3 cm o.d.) pipes. The SIPS concept appears attractive for pretreating high level wastes, since it would: (1) process waste in-situ in the tanks, (2) be cheaper and more reliable than a larger centralized facility, (3) be quickly demonstrable at full scale, (4) have less technical risk, (5) avoid having to transfer unstable slurries for long distances, and (6) be simple to decommission and dispose of. Further investigation of the SIPS concept appears desirable, including experimental testing and development of subscale demonstration units.

  2. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bell, Ronald E.

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ? 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, andmore »wavelength calibration.« less

  3. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera

    SciTech Connect (OSTI)

    Bell, Ronald E.

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ? 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  4. Development and Operation of High-throughput Accurate-wavelength Lens-based Spectrometer

    SciTech Connect (OSTI)

    Bell, Ronald E

    2014-07-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy < 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  5. Development and operation of a high-throughput accurate-wavelength lens-based spectrometer

    SciTech Connect (OSTI)

    Bell, Ronald E.

    2014-11-15

    A high-throughput spectrometer for the 400–820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm{sup ?1} grating is matched with fast f/1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy ?0.075 arc sec. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount at the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  6. Development and operation of a high-throughput accurate-wavelength lens-based spectrometera)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bell, Ronald E. [Princeton Plasma Physics Laboratory, Princeton, New Jersey 08543, USA

    2014-11-01

    A high-throughput spectrometer for the 400-820 nm wavelength range has been developed for charge exchange recombination spectroscopy or general spectroscopy. A large 2160 mm-1 grating is matched with fast f /1.8 200 mm lenses, which provide stigmatic imaging. A precision optical encoder measures the grating angle with an accuracy < 0.075 arc seconds. A high quantum efficiency low-etaloning CCD detector allows operation at longer wavelengths. A patch panel allows input fibers to interface with interchangeable fiber holders that attach to a kinematic mount behind the entrance slit. Computer-controlled hardware allows automated control of wavelength, timing, f-number, automated data collection, and wavelength calibration.

  7. PIPA: A High-Throughput Pipeline for Protein Function Annotation Chenggang Yu, Valmik Desai, Nela Zavaljevski, and Jaques Reifman

    E-Print Network [OSTI]

    PIPA: A High-Throughput Pipeline for Protein Function Annotation Chenggang Yu, Valmik Desai, Nela of multisource predictions. We developed Pipeline for Protein Annotation (PIPA), a genome-wide protein function annotation pipeline that runs in a high-performance computing environment. PIPA integrates different tools

  8. Integrated crystal mounting and alignment system for high-throughput biological crystallography

    DOE Patents [OSTI]

    Nordmeyer, Robert A.; Snell, Gyorgy P.; Cornell, Earl W.; Kolbe, William; Yegian, Derek; Earnest, Thomas N.; Jaklevic, Joseph M.; Cork, Carl W.; Santarsiero, Bernard D.; Stevens, Raymond C.

    2005-07-19

    A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

  9. Thicker, more efficient superconducting strip-line detectors for high throughput macromolecules analysis

    SciTech Connect (OSTI)

    Casaburi, A.; Ejrnaes, M.; Cristiano, R.; Zen, N.; Ohkubo, M.; Pagano, S.

    2011-01-10

    Fast detectors with large area are required in time-of-flight mass spectrometers for high throughput analysis of biological molecules. We fabricated and characterized subnanosecond 1x1 mm{sup 2} NbN superconducting strip-line detectors. The influence of the strip-line thickness on the temporal characteristics and efficiency of the detector for the impacts of keV accelerated molecules is investigated. We find that the increase of thickness improves both efficiency and response time. In the thicker sample we achieved a rise time of 380 ps, a fall time of 1.38 ns, and a higher count rate. The physics involved in this behavior is investigated.

  10. Integrated crystal mounting and alignment system for high-throughput biological crystallography

    DOE Patents [OSTI]

    Nordmeyer, Robert A. (San Leandro, CA); Snell, Gyorgy P. (Richmond, CA); Cornell, Earl W. (Antioch, CA); Kolbe, William F. (Moraga, CA); Yegian, Derek T. (Oakland, CA); Earnest, Thomas N. (Berkeley, CA); Jaklevich, Joseph M. (Lafayette, CA); Cork, Carl W. (Walnut Creek, CA); Santarsiero, Bernard D. (Chicago, IL); Stevens, Raymond C. (La Jolla, CA)

    2007-09-25

    A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

  11. Metalloproteomics: High-Throughput Structural and Functional Annotation of Proteins in Structural Genomics

    SciTech Connect (OSTI)

    Shi,W.; Zhan, C.; Lgnatov, A.; Manjasetty, B.; Marinkovic, N.; Sullivan, M.; Huang, R.; Chance, M.; Li, H.; et al.

    2005-01-01

    A high-throughput method for measuring transition metal content based on quantitation of X-ray fluorescence signals was used to analyze 654 proteins selected as targets by the New York Structural GenomiX Research Consortium. Over 10% showed the presence of transition metal atoms in stoichiometric amounts; these totals as well as the abundance distribution are similar to those of the Protein Data Bank. Bioinformatics analysis of the identified metalloproteins in most cases supported the metalloprotein annotation; identification of the conserved metal binding motif was also shown to be useful in verifying structural models of the proteins. Metalloproteomics provides a rapid structural and functional annotation for these sequences and is shown to be {approx}95% accurate in predicting the presence or absence of stoichiometric metal content. The project's goal is to assay at least 1 member from each Pfam family; approximately 500 Pfam families have been characterized with respect to transition metal content so far.

  12. High-throughput generation, optimization and analysis of genome-scale metabolic models.

    SciTech Connect (OSTI)

    Henry, C. S.; DeJongh, M.; Best, A. A.; Frybarger, P. M.; Linsay, B.; Stevens, R. L.

    2010-09-01

    Genome-scale metabolic models have proven to be valuable for predicting organism phenotypes from genotypes. Yet efforts to develop new models are failing to keep pace with genome sequencing. To address this problem, we introduce the Model SEED, a web-based resource for high-throughput generation, optimization and analysis of genome-scale metabolic models. The Model SEED integrates existing methods and introduces techniques to automate nearly every step of this process, taking {approx}48 h to reconstruct a metabolic model from an assembled genome sequence. We apply this resource to generate 130 genome-scale metabolic models representing a taxonomically diverse set of bacteria. Twenty-two of the models were validated against available gene essentiality and Biolog data, with the average model accuracy determined to be 66% before optimization and 87% after optimization.

  13. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2015-01-01

    An acoustic high-throughput screening method is described for harvesting protein crystals and combining the protein crystals with chemicals such as a fragment library. Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s{sup ?1}) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  14. been the key to their success is the use of high-throughput experiments to assess various zeo-

    E-Print Network [OSTI]

    Silver, Whendee

    it and the resulting pore structure. It could be that the hexamethonium molecules pack in such a way as to providebeen the key to their success is the use of high- throughput experiments to assess various zeo and shape of the resulting pores cor- responds to the size and shape of the organic molecule. ITQ-33

  15. Implementation of a High-Throughput Screen for Identifying Small Molecules to Activate the Keap1-Nrf2-ARE Pathway

    E-Print Network [OSTI]

    Wu, Kai Connie; McDonald, Peter R.; Liu, Jie Jerry; Chaguturu, Rathnam; Klaassen, Curtis D.

    2012-10-08

    of these genes. To identify Nrf2 activators for the treatment of oxidative/electrophilic stress-induced diseases, the present study developed a high-throughput assay to evaluate Nrf2 activation using AREc32 cells that contain a luciferase gene under the control...

  16. PIPELINED PARALLEL ARCHITECTURE FOR HIGH THROUGHPUT MAP DETECTORS Ruwan Ratnayake, Gu-Yeon Wei and Aleksandar Kavcic

    E-Print Network [OSTI]

    Kavcic, Aleksandar

    PIPELINED PARALLEL ARCHITECTURE FOR HIGH THROUGHPUT MAP DETECTORS Ruwan Ratnayake, Gu-Yeon Wei performance close to the channel capacity limits. Deep pipelined architecture for the forward only method, where as FB-BCJR only allow sequential state metric computations. Parallelism facilitates pipelining, re

  17. A GPU-INSPIRED SOFT PROCESSOR FOR HIGH-THROUGHPUT ACCELERATION Jeffrey Kingyens and J. Gregory Steffan

    E-Print Network [OSTI]

    Steffan, J. Gregory

    ) is realizable on an XtremeData XD1000 FPGA-based accelerator system, we demonstrate that our soft processor can in the scientific, financial, and other data-driven arenas that will use them. Developing an accelerator designA GPU-INSPIRED SOFT PROCESSOR FOR HIGH-THROUGHPUT ACCELERATION Jeffrey Kingyens and J. Gregory

  18. Novel mixed polyanions lithium-ion battery cathode materials predicted by high-throughput ab initio computations

    E-Print Network [OSTI]

    Ceder, Gerbrand

    Novel mixed polyanions lithium-ion battery cathode materials predicted by high-throughput ab initio (>700 Wh/kg) cathode materials for lithium-ion batteries. 1 Introduction The widespread use of lithium compounds. Testing previously known lithium-containing compounds for battery properties can lead

  19. A Cell-Based High-Throughput Screen for Novel Chemical Inducers of Fetal Hemoglobin for Treatment of Hemoglobinopathies

    E-Print Network [OSTI]

    Peterson, Kenneth R.; Costa, Flá via C.; Fedosyuk, Halyna; Neades, Renee Y.; Chazelle, Allen M.; Zelenchuk, Lesya; Fonteles, Andrea H.; Dalal, Parmita; Roy, Anuradha; Chaguturu, Rathnam; Li, Biaoru; Pace, Betty S.

    2014-09-16

    , with minimal off-target effects, continues to be an elusive goal. One hurdle has been an assay amenable to a high-throughput screen (HTS) of chemicals that displays a robust c-globin off-on switch to identify potential lead compounds. Assay systems developed...

  20. High-throughput liquid-absorption air-sampling apparatus and methods

    DOE Patents [OSTI]

    Zaromb, Solomon (95706 William Dr., Hinsdale, IL 60521)

    2000-01-01

    A portable high-throughput liquid-absorption air sampler [PHTLAAS] has an asymmetric air inlet through which air is drawn upward by a small and light-weight centrifugal fan driven by a direct current motor that can be powered by a battery. The air inlet is so configured as to impart both rotational and downward components of motion to the sampled air near said inlet. The PHTLAAS comprises a glass tube of relatively small size through which air passes at a high rate in a swirling, highly turbulent motion, which facilitates rapid transfer of vapors and particulates to a liquid film covering the inner walls of the tube. The pressure drop through the glass tube is <10 cm of water, usually <5 cm of water. The sampler's collection efficiency is usually >20% for vapors or airborne particulates in the 2-3.mu. range and >50% for particles larger than 4.mu.. In conjunction with various analyzers, the PHTLAAS can serve to monitor a variety of hazardous or illicit airborne substances, such as lead-containing particulates, tritiated water vapor, biological aerosols, or traces of concealed drugs or explosives.

  1. SPONTANEOUS CATALYTIC WET AIR OXIDATION DURING PRE-TREATMENT OF HIGH-LEVEL RADIOACTIVE WASTE SLUDGE

    SciTech Connect (OSTI)

    Koopman, D.; Herman, C.; Pareizs, J.; Bannochie, C.; Best, D.; Bibler, N.; Fellinger, T.

    2009-10-01

    Savannah River Remediation, LLC (SRR) operates the Defense Waste Processing Facility for the U.S. Department of Energy at the Savannah River Site. This facility immobilizes high-level radioactive waste through vitrification following chemical pretreatment. Catalytic destruction of formate and oxalate ions to carbon dioxide has been observed during qualification testing of non-radioactive analog systems. Carbon dioxide production greatly exceeded hydrogen production, indicating the occurrence of a process other than the catalytic decomposition of formic acid. Statistical modeling was used to relate the new reaction chemistry to partial catalytic wet air oxidation of both formate and oxalate ions driven by the low concentrations of palladium, rhodium, and/or ruthenium in the waste. Variations in process conditions led to increases or decreases in the total oxidative destruction, as well as partially shifting the preferred species undergoing destruction from oxalate ion to formate ion.

  2. A search model for topological insulators with high-throughput robustness descriptors

    SciTech Connect (OSTI)

    Yang, Kesong; Setyawan, Wahyu; Wang, Shidong; Nardelli, Marco B.; Curtarolo, Stephano

    2012-05-13

    Topological insulators (TI) are becoming one of the most studied classes of novel materials because of their great potential for applications ranging from spintronics to quantum computers. To fully integrate TI materials in electronic devices, high-quality epitaxial single-crystalline phases with sufficiently large bulk bandgaps are necessary. Current efforts have relied mostly on costly and time-consuming trial-and-error procedures. Here we show that by defining a reliable and accessible descriptor {cflx X}TI, which represents the topological robustness or feasibility of the candidate, and by searching the quantum materials repository aflowlib.org, we have automatically discovered 28 TIs (some of them already known) in five different symmetry families. These include peculiar ternary halides, Cs{l_brace}Sn,Pb,Ge{r_brace}{l_brace}Cl,Br,I{r_brace}{sub 3}, which could have been hardly anticipated without high-throughput means. Our search model, by relying on the significance of repositories in materials development, opens new avenues for the discovery of more TIs in different and unexplored classes of systems.

  3. FBI Fingerprint Image Capture System High-Speed-Front-End throughput modeling

    SciTech Connect (OSTI)

    Rathke, P.M.

    1993-09-01

    The Federal Bureau of Investigation (FBI) has undertaken a major modernization effort called the Integrated Automated Fingerprint Identification System (IAFISS). This system will provide centralized identification services using automated fingerprint, subject descriptor, mugshot, and document processing. A high-speed Fingerprint Image Capture System (FICS) is under development as part of the IAFIS program. The FICS will capture digital and microfilm images of FBI fingerprint cards for input into a central database. One FICS design supports two front-end scanning subsystems, known as the High-Speed-Front-End (HSFE) and Low-Speed-Front-End, to supply image data to a common data processing subsystem. The production rate of the HSFE is critical to meeting the FBI`s fingerprint card processing schedule. A model of the HSFE has been developed to help identify the issues driving the production rate, assist in the development of component specifications, and guide the evolution of an operations plan. A description of the model development is given, the assumptions are presented, and some HSFE throughput analysis is performed.

  4. Metal Organic Framework Research: High Throughput Discovery of Robust Metal Organic Framework for CO2 Capture

    SciTech Connect (OSTI)

    None

    2010-08-01

    IMPACCT Project: LBNL is developing a method for identifying the best metal organic frameworks for use in capturing CO2 from the flue gas of coal-fired power plants. Metal organic frameworks are porous, crystalline compounds that, based on their chemical structure, vary considerably in terms of their capacity to grab hold of passing CO2 molecules and their ability to withstand the harsh conditions found in the gas exhaust of coal-fired power plants. Owing primarily to their high tunability, metal organic frameworks can have an incredibly wide range of different chemical and physical properties, so identifying the best to use for CO2 capture and storage can be a difficult task. LBNL uses high-throughput instrumentation to analyze nearly 100 materials at a time, screening them for the characteristics that optimize their ability to selectively adsorb CO2 from coal exhaust. Their work will identify the most promising frameworks and accelerate their large-scale commercial development to benefit further research into reducing the cost of CO2 capture and storage.

  5. Evaluation of a New Remote Handling Design for High Throughput Annular Centrifugal Contactors

    SciTech Connect (OSTI)

    David H. Meikrantz; Troy G. Garn; Jack D. Law; Lawrence L. Macaluso

    2009-09-01

    Advanced designs of nuclear fuel recycling plants are expected to include more ambitious goals for aqueous based separations including; higher separations efficiency, high-level waste minimization, and a greater focus on continuous processes to minimize cost and footprint. Therefore, Annular Centrifugal Contactors (ACCs) are destined to play a more important role for such future processing schemes. Previous efforts defined and characterized the performance of commercial 5 cm and 12.5 cm single-stage ACCs in a “cold” environment. The next logical step, the design and evaluation of remote capable pilot scale ACCs in a “hot” or radioactive environment was reported earlier. This report includes the development of remote designs for ACCs that can process the large throughput rates needed in future nuclear fuel recycling plants. Novel designs were developed for the remote interconnection of contactor units, clean-in-place and drain connections, and a new solids removal collection chamber. A three stage, 12.5 cm diameter rotor module has been constructed and evaluated for operational function and remote handling in highly radioactive environments. This design is scalable to commercial CINC ACC models from V-05 to V-20 with total throughput rates ranging from 20 to 650 liters per minute. The V-05R three stage prototype was manufactured by the commercial vendor for ACCs in the U.S., CINC mfg. It employs three standard V-05 clean-in-place (CIP) units modified for remote service and replacement via new methods of connection for solution inlets, outlets, drain and CIP. Hydraulic testing and functional checks were successfully conducted and then the prototype was evaluated for remote handling and maintenance suitability. Removal and replacement of the center position V-05R ACC unit in the three stage prototype was demonstrated using an overhead rail mounted PaR manipulator. This evaluation confirmed the efficacy of this innovative design for interconnecting and cleaning individual stages while retaining the benefits of commercially reliable ACC equipment for remote applications in the nuclear industry. Minor modifications and suggestions for improved manual remote servicing by the remote handling specialists were provided but successful removal and replacement was demonstrated in the first prototype.

  6. FELIX: a High-Throughput Network Approach for Interfacing to Front End Electronics for ATLAS Upgrades

    E-Print Network [OSTI]

    Anderson, John Thomas; The ATLAS collaboration; Boterenbrood, Hendrik; Chen, Hucheng; Chen, Kai; Drake, Gary; Francis, David; Gorini, Benedetto; Lanni, Francesco; Lehmann Miotto, Giovanna; Levinson, Lorne; Narevicius, Julia; Plessl, Christian; Roich, Alexander; Ryu, Soo; Schreuder, Frans Philip; Schumacher, Jorn; Vandelli, Wainer; Vermeulen, Jos; Zhang, Jinlong

    2015-01-01

    The ATLAS experiment at CERN is planning full deployment of a new unified optical link technology for connecting detector front end electronics on the timescale of the LHC Run 4 (2025). It is estimated that roughly 8000 GBT (GigaBit Transceiver) links, with transfer rates up to 10.24~Gbps, will replace existing links used for readout, detector control and distribution of timing and trigger information. A new class of devices will be needed to interface many GBT links to the rest of the trigger, data-acquisition and detector control systems. In this paper FELIX (Front End LInk eXchange) is presented, a PC-based device to route data from and to multiple GBT links via a high-performance general purpose network capable of a total throughput up to O(20 Tbps). FELIX implies architectural changes to the ATLAS data acquisition system, such as the use of industry standard COTS components early in the DAQ chain. Additionally the design and implementation of a FELIX demonstration platform is presented, and hardware and ...

  7. Gasification of refuse derived fuel in the Battelle high throughput gasification system

    SciTech Connect (OSTI)

    Paisley, M.A.; Creamer, K.S.; Tweksbury, T.L.; Taylor, D.R. )

    1989-07-01

    This report presents the results of an experimental program to demonstrate the suitability of the Battelle High Throughput Gasification Process to non-wood biomass fuels. An extensive data base on wood gasification was generated during a multi-year experimental program. This data base and subsequent design and economic analysis activities led to the discussion to study the gasification character of other fuels. The specific fuel studied was refuse derived fuel (RDF) which is a prepared municipal solid waste (MSW). The use of RDF, while providing a valuable fuel, can also provide a solution to MSW disposal problems. Gasification of MSW provides advantages over land fill or mass burn technology since a more usable form of energy, medium Btu gas, is produced. Land filling of wastes produces no usable products and mass burning while greatly reducing the volume of wastes for disposal can produce only steam. This steam must be used on site or very nearby this limiting the potential locations for mass burn facilities. Such a gas, if produced from currently available supplies of MSW, can contribute 2 quads to the US energy supply. 3 refs., 12 figs., 7 tabs.

  8. A microreactor array for spatially resolved measurement of catalytic activity for high-throughput catalysis science

    SciTech Connect (OSTI)

    Kondratyuk, Petro; Gumuslu, Gamze; Shukla, Shantanu; Miller, James B.; Morreale, Bryan D.; Gellman, Andrew J.

    2013-04-01

    We describe a 100 channel microreactor array capable of spatially resolved measurement of catalytic activity across the surface of a flat substrate. When used in conjunction with a composition spread alloy film (CSAF, e.g. Pd{sub x}Cu{sub y}Au{sub 1-x-y}) across which component concentrations vary smoothly, such measurements permit high-throughput analysis of catalytic activity and selectivity as a function of catalyst composition. In the reported implementation, the system achieves spatial resolution of 1 mm{sup 2} over a 10×10 mm{sup 2} area. During operation, the reactant gases are delivered at constant flow rate to 100 points of differing composition on the CSAF surface by means of a 100-channel microfluidic device. After coming into contact with the CSAF catalyst surface, the product gas mixture from each of the 100 points is withdrawn separately through a set of 100 isolated channels for analysis using a mass spectrometer. We demonstrate the operation of the device on a Pd{sub x}Cu{sub y}Au{sub 1-x-y} CSAF catalyzing the H{sub 2}-D{sub 2} exchange reaction at 333 K. In essentially a single experiment, we measured the catalytic activity over a broad swathe of concentrations from the ternary composition space of the Pd{sub x}Cu{sub y}Au{sub 1-x-y} alloy.

  9. Co-hydrolysis of hydrothermal and dilute acid pretreated Populus slurries to support development of a high-throughput pretreatment system

    E-Print Network [OSTI]

    Studer, Michael H; Brethauer, Simone; DeMartini, Jaclyn D; McKenzie, Heather L; Wyman, Charles E

    2011-01-01

    process samples. National Renewable Energy Laboratory, NREL/at the National Renewable Energy Laboratory for approxi-the standard National Renewable Energy Laboratory method [

  10. Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

    SciTech Connect (OSTI)

    Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K.; Hjort, Klas

    2009-01-05

    We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.

  11. 'Sifting the significance from the data' - the impact of high-throughput genomic technologies on human genetics and health care

    E-Print Network [OSTI]

    Clarke, Angus J.; Cooper, David N.; Krawczak, Michael; Tyler-Smith, Chris; Wallace, Helen M.; Wilkie, Andrew O. M.; Raymond, Frances L.; Chadwick, Ruth; Craddock, Nick; John, Ros; Gallacher, John; Chiano, Mathias

    2012-08-02

    and ageing), and in response to environmental challenges (diet, stress). To this end, a number of ingenious genome-wide, high- throughput technologies have been developed. Some are based on the ability to selectively capture methylated fragments of the genome... consumes too much energy or mis- conceived arguments lead to the inappropriate applica- tion of theory in policy and practise across various walks of life, most especially psychiatry, education and the law. Second, and despite a full acceptance...

  12. Synthesis and characterization of four new metal 5-phosphonoisophthalates discovered by high-throughput experimentation

    SciTech Connect (OSTI)

    Bauer, Sebastian [Institute of Inorganic Chemistry, Christian-Albrechts-University, Otto-Hahn-Platz 6/7, D 24098 Kiel (Germany); Stock, Norbert [Institute of Inorganic Chemistry, Christian-Albrechts-University, Otto-Hahn-Platz 6/7, D 24098 Kiel (Germany)], E-mail: stock@ac.uni-kiel.de

    2007-11-15

    A new ligand, 5-diethylphosphonoisophthalic acid ((HOOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}(C{sub 2}H{sub 5}){sub 2}, H{sub 2}Et{sub 2}L), for the hydrothermal synthesis of inorganic-organic hybrid compounds was prepared and characterized by NMR-spectroscopy. Its in situ hydrolysis leads to the corresponding 5-phosphonoisophthalic acid ((HOOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}H{sub 2}, H{sub 4}L). Applying high-throughput methods, different di- and trivalent metal salts for the synthesis of crystalline metal phosphonates based on H{sub 2}Et{sub 2}L have been screened. From the resulting discovery library, single-crystals of four new compounds, [Sm{sub 2}(H{sub 2}O){sub 4}(H(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}){sub 2}].2H{sub 2}O (1), [Cu{sub 3}(H{sub 2}O)(H(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}){sub 2}].2H{sub 2}O (2), Ca{sub 2}(H{sub 2}O)[H(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}H]{sub 2} (3), and Ba{sub 2}(H{sub 2}O){sub 3}(OOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3} (4), have been isolated. The single-crystal structure determination of the title compounds shows H{sub 4}L to be a versatile ligand, exhibiting different types of coordination modes between the functional groups and the metal ions. A comparison of the structural features of the title compounds shows a varying degree of M-O-M connectivities. Thus, isolated metal-oxygen clusters (compounds 1 and 2), infinite M-O-M chains (compound 3), and infinite M-O-M layers (compound 4) are observed. The title compounds 1, 2, and 3 were further characterized by IR-spectroscopy, TG-, EDX-, and elemental chemical analysis. - Graphical abstract: Applying high-throughput methods, the new ligand 5-diethylphosphonoisophtalic acid, (HOOC){sub 2}C{sub 6}H{sub 3}-PO{sub 3}(C{sub 2}H{sub 5}){sub 2} (H{sub 2}Et{sub 2}L), was reacted with several di- and trivalent metal salts under hydrothermal conditions. Single-crystals of four new inorganic-organic hybrid compounds were isolated from the discovery library. The single-crystal structure analysis shows a varying M-O-M connectivity.

  13. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; et al

    2014-06-01

    Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from themore »inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.« less

  14. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

    SciTech Connect (OSTI)

    Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

    2014-06-01

    Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.

  15. Evaluation of high throughput screening methods in picking up differences between cultivars of

    E-Print Network [OSTI]

    California at Riverside, University of

    Sciences, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark c National Renewable Energy Laboratory, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH of combined eco- nomic benefits of higher yields at milder pretreatment condi- tions and lower enzyme dosages

  16. Biomass pretreatment

    DOE Patents [OSTI]

    Hennessey, Susan Marie; Friend, Julie; Elander, Richard T; Tucker, III, Melvin P

    2013-05-21

    A method is provided for producing an improved pretreated biomass product for use in saccharification followed by fermentation to produce a target chemical that includes removal of saccharification and or fermentation inhibitors from the pretreated biomass product. Specifically, the pretreated biomass product derived from using the present method has fewer inhibitors of saccharification and/or fermentation without a loss in sugar content.

  17. High-throughput two-hybrid analysis The promise and the peril

    E-Print Network [OSTI]

    Dunham, Maitreya

    , reduction in reagent costs and further refinements of the approach. As throughput escalates, so does use. The first stage is the initial description of a method: the prototype version. Typically. For a large fraction of new technologies, few other examples beyond this proto- type are ever described

  18. New Challenges of the Computation of Multiple Sequence Alignments in the High-Throughput Era (2010 JGI/ANL HPC Workshop)

    ScienceCinema (OSTI)

    Notredame, Cedric [Centre for Genomic Regulation

    2011-06-08

    Cedric Notredame from the Centre for Genomic Regulation gives a presentation on "New Challenges of the Computation of Multiple Sequence Alignments in the High-Throughput Era" at the JGI/Argonne HPC Workshop on January 26, 2010.

  19. Vehicle Technologies Office Merit Review 2015: High-Throughput Study of Diffusion and Phase Transformation Kinetics of Magnesium-Based Systems For Automotive Cast Magnesium Alloys

    Broader source: Energy.gov [DOE]

    Presentation given by Ohio State University at 2015 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about high-throughput study...

  20. Interleaving techniques for high-throughput chaotic noise generation in CMOS

    E-Print Network [OSTI]

    Sergio Callegari; Riccardo Rovatti; Gianluca Setti

    2015-07-01

    An interleaving technique is proposed to enhance the throughput of current-mode CMOS discrete time chaotic sources based on the iteration of unidimensional maps. A discussion of the reasons and the advantages offered by the approach is provided, together with analytical results about the conservation of some major statistical features. As an example, application to an FM-DCSK communication system is proposed. To conclude, a sample circuit capable of 20 Msample/s is presented.

  1. Laboratory Report on Performance Evaluation of Key Constituents during Pre-Treatment of High Level Waste Direct Feed

    SciTech Connect (OSTI)

    Huber, Heinz J.

    2013-06-24

    The analytical capabilities of the 222-S Laboratory are tested against the requirements for an optional start up scenario of the Waste Treatment and Immobilization Plant on the Hanford Site. In this case, washed and in-tank leached sludge would be sent directly to the High Level Melter, bypassing Pretreatment. The sludge samples would need to be analyzed for certain key constituents in terms identifying melter-related issues and adjustment needs. The analyses on original tank waste as well as on washed and leached material were performed using five sludge samples from tanks 241-AY-102, 241-AZ-102, 241-AN-106, 241-AW-105, and 241-SY-102. Additionally, solid phase characterization was applied to determine the changes in mineralogy throughout the pre-treatment steps.

  2. Assessment of advanced coal-gasification processes. [AVCO high throughput gasification in process; Bell High Mass Flux process; CS-R process; and Exxon Gasification process

    SciTech Connect (OSTI)

    McCarthy, J.; Ferrall, J.; Charng, T.; Houseman, J.

    1981-06-01

    This report represents a technical assessment of the following advanced coal gasification processes: AVCO High Throughput Gasification (HTG) Process, Bell Single - Stage High Mass Flux (HMF) Process, Cities Service/Rockwell (CS/R) Hydrogasification Process, and the Exxon Catalytic Coal Gasification (CCG) Process. Each process is evaluated for its potential to produce SNG from a bituminous coal. In addition to identifying the new technology these processes represent, key similarities/differences, strengths/weaknesses, and potential improvements to each process are identified. The AVCO HTG and the Bell HMF gasifiers share similarities with respect to: short residence time (SRT), high throughput rate, slagging and syngas as the initial raw product gas. The CS/R Hydrogasifier is also SRT but is non-slagging and produces a raw gas high in methane content. The Exxon CCG gasifier is a long residence time, catalytic fluidbed reactor producing all of the raw product methane in the gasifier.

  3. A Bayesian Approach to Calibrating High-Throughput Virtual Screening Results and Application to Organic Photovoltaic Materials

    E-Print Network [OSTI]

    Pyzer-Knapp, Edward O; Aspuru-Guzik, Alan

    2015-01-01

    A novel approach for calibrating quantum-chemical properties determined as part of a high-throughput virtual screen to experimental analogs is presented. Information on the molecular graph is extracted through the use of extended connectivity fingerprints, and exploited using a Gaussian process to calibrate both electronic properties such as frontier orbital energies, and optical gaps and device properties such as short circuit current density, open circuit voltage and power conversion efficiency. The Bayesian nature of this process affords a value for uncertainty in addition to each calibrated value. This allows the researcher to gain intuition about the model as well as the ability to respect its bounds.

  4. High energy x-ray diffraction/x-ray fluorescence spectroscopy for high-throughput analysis of composition spread thin films

    SciTech Connect (OSTI)

    Gregoire, John M. [Department of Physics, and Cornell Fuel Cell Institute, Cornell University, Ithaca, New York 14853 (United States); Dale, Darren; Kazimirov, Alexander [Cornell High Energy Synchrotron Source, Cornell University, New York 14853 (United States); DiSalvo, Francis J. [Department of Chemistry and Chemical Biology, and Cornell Fuel Cell Institute, Cornell University, New York 14853 (United States); Dover, R. Bruce van [Department of Materials Science and Engineering, and Cornell Fuel Cell Institute, Cornell University, New York 14853 (United States)

    2009-12-15

    High-throughput crystallography is an important tool in materials research, particularly for the rapid assessment of structure-property relationships. We present a technique for simultaneous acquisition of diffraction images and fluorescence spectra on a continuous composition spread thin film using a 60 keV x-ray source. Subsequent noninteractive data processing provides maps of the diffraction profiles, thin film fiber texture, and composition. Even for highly textured films, our diffraction technique provides detection of diffraction from each family of Bragg reflections, which affords direct comparison of the measured profiles with powder patterns of known phases. These techniques are important for high throughput combinatorial studies as they provide structure and composition maps which may be correlated with performance trends within an inorganic library.

  5. RECENT PROCESS AND EQUIPMENT IMPROVEMENTS TO INCREASE HIGH LEVEL WASTE THROUGHPUT AT THE DEFENSE WASTE PROCESSING FACILITY

    SciTech Connect (OSTI)

    Odriscoll, R; Allan Barnes, A; Jim Coleman, J; Timothy Glover, T; Robert Hopkins, R; Dan Iverson, D; Jeff Leita, J

    2008-01-15

    The Savannah River Site's (SRS) Defense Waste Processing Facility (DWPF) began stabilizing high level waste (HLW) in a glass matrix in 1996. Over the past few years, there have been several process and equipment improvements at the DWPF to increase the rate at which the high level waste can be stabilized. These improvements have either directly increased waste processing rates or have desensitized the process to upsets, thereby minimizing downtime and increasing production. Improvements due to optimization of waste throughput with increased HLW loading of the glass resulted in a 6% waste throughput increase based upon operational efficiencies. Improvements in canister production include the pour spout heated bellows liner (5%), glass surge (siphon) protection software (2%), melter feed pump software logic change to prevent spurious interlocks of the feed pump with subsequent dilution of feed stock (2%) and optimization of the steam atomized scrubber (SAS) operation to minimize downtime (3%) for a total increase in canister production of 12%. A number of process recovery efforts have allowed continued operation. These include the off gas system pluggage and restoration, slurry mix evaporator (SME) tank repair and replacement, remote cleaning of melter top head center nozzle, remote melter internal inspection, SAS pump J-Tube recovery, inadvertent pour scenario resolutions, dome heater transformer bus bar cooling water leak repair and new Infra-red camera for determination of glass height in the canister are discussed.

  6. Risk-based high-throughput chemical screening and prioritization using exposure models and in vitro bioactivity assays

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shin, Hyeong -Moo; Ernstoff, Alexi; Arnot, Jon A.; Wetmore, Barbara A.; Csiszar, Susan A.; Fantke, Peter; Zhang, Xianming; McKone, Thomas E.; Jolliet, Olivier; Bennett, Deborah H.

    2015-05-01

    We present a risk-based high-throughput screening (HTS) method to identify chemicals for potential health concerns or for which additional information is needed. The method is applied to 180 organic chemicals as a case study. We first obtain information on how the chemical is used and identify relevant use scenarios (e.g., dermal application, indoor emissions). For each chemical and use scenario, exposure models are then used to calculate a chemical intake fraction, or a product intake fraction, accounting for chemical properties and the exposed population. We then combine these intake fractions with use scenario-specific estimates of chemical quantity to calculate dailymore »intake rates (iR; mg/kg/day). These intake rates are compared to oral equivalent doses (OED; mg/kg/day), calculated from a suite of ToxCast in vitro bioactivity assays using in vitro-to-in vivo extrapolation and reverse dosimetry. Bioactivity quotients (BQs) are calculated as iR/OED to obtain estimates of potential impact associated with each relevant use scenario. Of the 180 chemicals considered, 38 had maximum iRs exceeding minimum OEDs (i.e., BQs > 1). For most of these compounds, exposures are associated with direct intake, food/oral contact, or dermal exposure. The method provides high-throughput estimates of exposure and important input for decision makers to identify chemicals of concern for further evaluation with additional information or more refined models.« less

  7. High-throughput, dual probe biological assays based on single molecule detection

    DOE Patents [OSTI]

    Hollars, Christopher W. (Brentwood, CA); Huser, Thomas R. (Livermore, CA); Lane, Stephen M. (Oakland, CA); Balhorn, Rodney L. (Livermore, CA); Bakajin, Olgica (San Leandro, CA); Darrow, Christopher (Pleasanton, CA); Satcher, Jr., Joe H. (Patterson, CA)

    2006-07-11

    A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 .mu.m.times.100 .mu.m.times.100 .mu.m. A device using these methods would have 10.sup.3 10.sup.4 greater throughput than previous one-dimensional (1D) micro-stream devices having 1 .mu.m.sup.3 interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (.about.10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

  8. SPATIALLY-SEPARATED ATOMIC LAYER DEPOSITION OF Al2O3, A NEW OPTION FOR HIGH-THROUGHPUT Si SOLAR CELL PASSIVATION

    E-Print Network [OSTI]

    SPATIALLY-SEPARATED ATOMIC LAYER DEPOSITION OF Al2O3, A NEW OPTION FOR HIGH-THROUGHPUT Si SOLAR material for surface passivation of crystalline Si is atomic layer deposited (ALD) Al2O3. However for the development of high-efficiency solar cells. It has been shown that atomic layer deposition (ALD) of Al2O3

  9. Industrial co-generation through use of a medium BTU gas from biomass produced in a high throughput reactor

    SciTech Connect (OSTI)

    Feldmann, H.F.; Ball, D.A.; Paisley, M.A.

    1983-01-01

    A high-throughput gasification system has been developed for the steam gasification of woody biomass to produce a fuel gas with a heating value of 475 to 500 Btu/SCF without using oxygen. Recent developments have focused on the use of bark and sawdust as feedstocks in addition to wood chips and the testing of a new reactor concept, the so-called controlled turbulent zone (CTZ) reactor to increase gas production per unit of wood fed. Operating data from the original gasification system and the CTZ system are used to examine the preliminary economics of biomass gasification/gas turbine cogeneration systems. In addition, a ''generic'' pressurized oxygen-blown gasification system is evaluated. The economics of these gasification systems are compared with a conventional wood boiler/steam turbine cogeneration system.

  10. Determination of gene expression patterns using high-throughput RNA in situ hybridizaion to whole-mount Drosophila embryos

    SciTech Connect (OSTI)

    Weiszmann, R.; Hammonds, A.S.; Celniker, S.E.

    2009-04-09

    We describe a high-throughput protocol for RNA in situ hybridization (ISH) to Drosophila embryos in a 96-well format. cDNA or genomic DNA templates are amplified by PCR and then digoxigenin-labeled ribonucleotides are incorporated into antisense RNA probes by in vitro transcription. The quality of each probe is evaluated before ISH using a RNA probe quantification (dot blot) assay. RNA probes are hybridized to fixed, mixed-staged Drosophila embryos in 96-well plates. The resulting stained embryos can be examined and photographed immediately or stored at 4oC for later analysis. Starting with fixed, staged embryos, the protocol takes 6 d from probe template production through hybridization. Preparation of fixed embryos requires a minimum of 2 weeks to collect embryos representing all stages. The method has been used to determine the expression patterns of over 6,000 genes throughout embryogenesis.

  11. High-throughput identification of off-targets for the mechanistic study of severe adverse drug reactions induced by analgesics

    SciTech Connect (OSTI)

    Pan, Jian-Bo [Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005 (China); Ji, Nan; Pan, Wen; Hong, Ru [State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102 (China); Wang, Hao [Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005 (China); Ji, Zhi-Liang, E-mail: appo@xmu.edu.cn [State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102 (China); Department of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, Fujian 361005 (China)

    2014-01-01

    Drugs may induce adverse drug reactions (ADRs) when they unexpectedly bind to proteins other than their therapeutic targets. Identification of these undesired protein binding partners, called off-targets, can facilitate toxicity assessment in the early stages of drug development. In this study, a computational framework was introduced for the exploration of idiosyncratic mechanisms underlying analgesic-induced severe adverse drug reactions (SADRs). The putative analgesic-target interactions were predicted by performing reverse docking of analgesics or their active metabolites against human/mammal protein structures in a high-throughput manner. Subsequently, bioinformatics analyses were undertaken to identify ADR-associated proteins (ADRAPs) and pathways. Using the pathways and ADRAPs that this analysis identified, the mechanisms of SADRs such as cardiac disorders were explored. For instance, 53 putative ADRAPs and 24 pathways were linked with cardiac disorders, of which 10 ADRAPs were confirmed by previous experiments. Moreover, it was inferred that pathways such as base excision repair, glycolysis/glyconeogenesis, ErbB signaling, calcium signaling, and phosphatidyl inositol signaling likely play pivotal roles in drug-induced cardiac disorders. In conclusion, our framework offers an opportunity to globally understand SADRs at the molecular level, which has been difficult to realize through experiments. It also provides some valuable clues for drug repurposing. - Highlights: • A novel computational framework was developed for mechanistic study of SADRs. • Off-targets of drugs were identified in large scale and in a high-throughput manner. • SADRs like cardiac disorders were systematically explored in molecular networks. • A number of ADR-associated proteins were identified.

  12. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control{

    E-Print Network [OSTI]

    Sinskey, Anthony J.

    Microbioreactor arrays with integrated mixers and fluid injectors for high- throughput, with 100 mL working volume, comprising a peristaltic oxygenating mixer and microfluidic injectors litre reference fermentation, conducted with the same strain, in a bench scale stirred tank bioreactor

  13. Ultra-high Throughput Real-time Instruments for Capturing Fast Signals and Rare Events

    E-Print Network [OSTI]

    Buckley, Brandon Walter

    2013-01-01

    fiber; OSA: Optical spectrum analyzer; PD: Photo-detector;high-speed optical spectrum analyzer [60]. We call this1% tap on an optical spectrum analyzer (OSA). The resulting

  14. Development and operation of a high-throughput accurate-wavelength...

    Office of Scientific and Technical Information (OSTI)

    imaging. A precision optical encoder measures the grating angle with an accuracy 0.075 arc sec. A high quantum efficiency low-etaloning CCD detector allows operation at longer...

  15. High-throughput screening of perovskite alloys for piezoelectric performance and thermodynamic stability

    E-Print Network [OSTI]

    Armiento, Rickard R.

    We screen a large chemical space of perovskite alloys for systems with optimal properties to accommodate a morphotropic phase boundary (MPB) in their composition-temperature phase diagram, a crucial feature for high ...

  16. AFLOW: An automatic framework for high-throughput materials discovery Stefano Curtarolo a,b,

    E-Print Network [OSTI]

    Curtarolo, Stefano

    , Denis O. Demchenko f , Dane Morgan g a Department of Mechanical Engineering and Materials Science, Duke Commonwealth University, Richmond, VA 23284, United States g Department of Materials Science and Engineering-optimization techniques used for the study of high-temperature reactions in multicomponent hydrides [29­31]. In its

  17. Applications of high throughput (combinatorial) methodologies to electronic, magnetic, optical, and energy-related materials

    E-Print Network [OSTI]

    Rubloff, Gary W.

    , and energy-related materials Martin L. Green, Ichiro Takeuchi, and Jason R. Hattrick-Simpers Citation: J) methodologies to electronic, magnetic, optical, and energy-related materials Martin L. Green,1 Ichiro Takeuchi,2 materials science for electronic, magnetic, optical, and energy-related materials. It is expected that high

  18. Air-stable ink for scalable, high-throughput layer deposition

    DOE Patents [OSTI]

    Weil, Benjamin D; Connor, Stephen T; Cui, Yi

    2014-02-11

    A method for producing and depositing air-stable, easily decomposable, vulcanized ink on any of a wide range of substrates is disclosed. The ink enables high-volume production of optoelectronic and/or electronic devices using scalable production methods, such as roll-to-roll transfer, fast rolling processes, and the like.

  19. High-throughput film-densitometry: An efficient approach to generate large data sets

    SciTech Connect (OSTI)

    Typke, Dieter; Nordmeyer, Robert A.; Jones, Arthur; Lee, Juyoung; Avila-Sakar, Agustin; Downing, Kenneth H.; Glaeser, Robert M.

    2004-07-14

    A film-handling machine (robot) has been built which can, in conjunction with a commercially available film densitometer, exchange and digitize over 300 electron micrographs per day. Implementation of robotic film handling effectively eliminates the delay and tedium associated with digitizing images when data are initially recorded on photographic film. The modulation transfer function (MTF) of the commercially available densitometer is significantly worse than that of a high-end, scientific microdensitometer. Nevertheless, its signal-to-noise ratio (S/N) is quite excellent, allowing substantial restoration of the output to ''near-to-perfect'' performance. Due to the large area of the standard electron microscope film that can be digitized by the commercial densitometer (up to 10,000 x 13,680 pixels with an appropriately coded holder), automated film digitization offers a fast and inexpensive alternative to high-end CCD cameras as a means of acquiring large amounts of image data in electron microscopy.

  20. Scalable Computational Methods for the Analysis of High-Throughput Biological Data

    SciTech Connect (OSTI)

    Langston, Michael A

    2012-09-06

    This primary focus of this research project is elucidating genetic regulatory mechanisms that control an organism�¢����s responses to low-dose ionizing radiation. Although low doses (at most ten centigrays) are not lethal to humans, they elicit a highly complex physiological response, with the ultimate outcome in terms of risk to human health unknown. The tools of molecular biology and computational science will be harnessed to study coordinated changes in gene expression that orchestrate the mechanisms a cell uses to manage the radiation stimulus. High performance implementations of novel algorithms that exploit the principles of fixed-parameter tractability will be used to extract gene sets suggestive of co-regulation. Genomic mining will be performed to scrutinize, winnow and highlight the most promising gene sets for more detailed investigation. The overall goal is to increase our understanding of the health risks associated with exposures to low levels of radiation.

  1. High-Throughput/Combinatorial Techniques in Hydrogen Storage Materials R&D

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing Tool Fits the Bill Financing ToolSustainableSecurityHighDepartment ofLight-Emitting

  2. High-Throughput Methodology for Discovery of Metal-Organic Frameworks with

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancingR Walls - Building America Topa High Binding Energy (New

  3. High-Throughput/Combinatorial Techniques in Hydrogen Storage Materials R&D

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,ExecutiveFinancingR Walls - Building America Topa High Binding

  4. On-the-fly machine-learning for high-throughput experiments: search for rare-earth-free permanent magnets

    SciTech Connect (OSTI)

    Kusne, AG; Gao, TR; Mehta, A; Ke, LQ; Nguyen, MC; Ho, KM; Antropov, V; Wang, CZ; Kramer, MJ; Long, C; Takeuchi, I

    2014-09-15

    Advanced materials characterization techniques with ever-growing data acquisition speed and storage capabilities represent a challenge in modern materials science, and new procedures to quickly assess and analyze the data are needed. Machine learning approaches are effective in reducing the complexity of data and rapidly homing in on the underlying trend in multi-dimensional data. Here, we show that by employing an algorithm called the mean shift theory to a large amount of diffraction data in high-throughput experimentation, one can streamline the process of delineating the structural evolution across compositional variations mapped on combinatorial libraries with minimal computational cost. Data collected at a synchrotron beamline are analyzed on the fly, and by integrating experimental data with the inorganic crystal structure database (ICSD), we can substantially enhance the accuracy in classifying the structural phases across ternary phase spaces. We have used this approach to identify a novel magnetic phase with enhanced magnetic anisotropy which is a candidate for rare-earth free permanent magnet.

  5. High throughput lessons from the LHC experience.Johnston.TNC2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenry Bellamy, Ph.D. Title: Professor -|High energy neutronhigh

  6. GeoChip 3.0: A High Throughput Tool for Analyzing Microbial Community, Composition, Structure, and Functional Activity

    SciTech Connect (OSTI)

    He, Zhili; Deng, Ye; Nostrand, Joy Van; Tu, Qichao; Xu, Meiying; Hemme, Chris; Wu, Liyou; Hazen, Terry; Zhou, Jizhong; Li, Xingyuan; Gentry, Terry; Yin, Yifeng; Liebich, Jost

    2010-05-17

    Microarray-based genomic technology has been widely used for microbial community analysis, and it is expected that microarray-based genomic technologies will revolutionize the analysis of microbial community structure, function and dynamics. A new generation of functional gene arrays (GeoChip 3.0) has been developed, with 27,812 probes covering 56,990 gene variants from 292 functional gene families involved in carbon, nitrogen, phosphorus and sulfur cycles, energy metabolism, antibiotic resistance, metal resistance, and organic contaminant degradation. Those probes were derived from 2,744, 140, and 262 species for bacteria, archaea, and fungi, respectively. GeoChip 3.0 has several other distinct features, such as a common oligo reference standard (CORS) for data normalization and comparison, a software package for data management and future updating, and the gyrB gene for phylogenetic analysis. Our computational evaluation of probe specificity indicated that all designed probes had a high specificity to their corresponding targets. Also, experimental analysis with synthesized oligonucleotides and genomic DNAs showed that only 0.0036percent-0.025percent false positive rates were observed, suggesting that the designed probes are highly specific under the experimental conditions examined. In addition, GeoChip 3.0 was applied to analyze soil microbial communities in a multifactor grassland ecosystem in Minnesota, USA, which demonstrated that the structure, composition, and potential activity of soil microbial communities significantly changed with the plant species diversity. All results indicate that GeoChip 3.0 is a high throughput powerful tool for studying microbial community functional structure, and linking microbial communities to ecosystem processes and functioning. To our knowledge, GeoChip 3.0 is the most comprehensive microarrays currently available for studying microbial communities associated with geobiochemical cycling, global climate change, bioenergy, agricuture, land use, ecosystem management, environmental cleanup and restoration, bioreactor systems, and human health.

  7. Conversion of forest residues to a methane-rich gas in a high-throughput gasifier

    SciTech Connect (OSTI)

    Feldmann, H.F.; Paisley, M.A.; Appelbaum, H.R.; Taylor, D.R.

    1988-05-01

    Research was conducted in a process research unit to develop an entrained bed gasifier which is supplied heat by recirculating a stream of sand between a separate combustion vessel and the gasifier. The char remaining after gasification of the wood provides the fuel for the combustor. The research program was conducted in two phases. In the first phase, a 6 in. I.D. gasifier was used to establish the feasibility of the concept for a wide variety of biomass feeds. The second phase of the program was conducted with a 10 in. I.D. gasifier, and a fully automated feeder system, to evaluate gasifier performance at very high feed rates. The experimental results were used to develop design parameters and detailed energy and material balances for a conceptual plant. A preliminary cost analysis is presented in the report based on the conceptual design. 5 refs., 24 figs., 13 tabs.

  8. High-Throughput Genetic Analysis and Combinatorial Chiral Separations Based on Capillary Electrophoresis

    SciTech Connect (OSTI)

    Wenwan Zhong

    2003-08-05

    Capillary electrophoresis (CE) offers many advantages over conventional analytical methods, such as speed, simplicity, high resolution, low cost, and small sample consumption, especially for the separation of enantiomers. However, chiral method developments still can be time consuming and tedious. They designed a comprehensive enantioseparation protocol employing neutral and sulfated cyclodextrins as chiral selectors for common basic, neutral, and acidic compounds with a 96-capillary array system. By using only four judiciously chosen separation buffers, successful enantioseparations were achieved for 49 out of 54 test compounds spanning a large variety of pKs and structures. Therefore, unknown compounds can be screened in this manner to identify optimal enantioselective conditions in just one rn. In addition to superior separation efficiency for small molecules, CE is also the most powerful technique for DNA separations. Using the same multiplexed capillary system with UV absorption detection, the sequence of a short DNA template can be acquired without any dye-labels. Two internal standards were utilized to adjust the migration time variations among capillaries, so that the four electropherograms for the A, T, C, G Sanger reactions can be aligned and base calling can be completed with a high level of confidence. the CE separation of DNA can be applied to study differential gene expression as well. Combined with pattern recognition techniques, small variations among electropherograms obtained by the separation of cDNA fragments produced from the total RNA samples of different human tissues can be revealed. These variations reflect the differences in total RNA expression among tissues. Thus, this Ce-based approach can serve as an alternative to the DNA array techniques in gene expression analysis.

  9. Robofurnace: A semi-automated laboratory chemical vapor deposition system for high-throughput nanomaterial synthesis and process discovery

    SciTech Connect (OSTI)

    Oliver, C. Ryan; Westrick, William; Koehler, Jeremy; Brieland-Shoultz, Anna; Anagnostopoulos-Politis, Ilias; Cruz-Gonzalez, Tizoc [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States)] [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States); Hart, A. John, E-mail: ajhart@mit.edu [Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109 (United States); Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2013-11-15

    Laboratory research and development on new materials, such as nanostructured thin films, often utilizes manual equipment such as tube furnaces due to its relatively low cost and ease of setup. However, these systems can be prone to inconsistent outcomes due to variations in standard operating procedures and limitations in performance such as heating and cooling rates restrict the parameter space that can be explored. Perhaps more importantly, maximization of research throughput and the successful and efficient translation of materials processing knowledge to production-scale systems, relies on the attainment of consistent outcomes. In response to this need, we present a semi-automated lab-scale chemical vapor deposition (CVD) furnace system, called “Robofurnace.” Robofurnace is an automated CVD system built around a standard tube furnace, which automates sample insertion and removal and uses motion of the furnace to achieve rapid heating and cooling. The system has a 10-sample magazine and motorized transfer arm, which isolates the samples from the lab atmosphere and enables highly repeatable placement of the sample within the tube. The system is designed to enable continuous operation of the CVD reactor, with asynchronous loading/unloading of samples. To demonstrate its performance, Robofurnace is used to develop a rapid CVD recipe for carbon nanotube (CNT) forest growth, achieving a 10-fold improvement in CNT forest mass density compared to a benchmark recipe using a manual tube furnace. In the long run, multiple systems like Robofurnace may be linked to share data among laboratories by methods such as Twitter. Our hope is Robofurnace and like automation will enable machine learning to optimize and discover relationships in complex material synthesis processes.

  10. Pilot study of horizontal roughing filtration in northern Ghana as pretreatment for highly turbid dugout water

    E-Print Network [OSTI]

    Losleben, Tamar

    2008-01-01

    In Northern Region Ghana (NRG), highly turbid rainwater runoff and intermittent streams are collected in earthen dams called dugouts. These dams serve as many communities' main source of drinking and domestic water despite ...

  11. IMPACT OF NOBLE METALS AND MERCURY ON HYDROGEN GENERATION DURING HIGH LEVEL WASTE PRETREATMENT AT THE SAVANNAH RIVER SITE

    SciTech Connect (OSTI)

    Stone, M; Tommy Edwards, T; David Koopman, D

    2009-03-03

    The Defense Waste Processing Facility (DWPF) at the Savannah River Site vitrifies radioactive High Level Waste (HLW) for repository internment. The process consists of three major steps: waste pretreatment, vitrification, and canister decontamination/sealing. HLW consists of insoluble metal hydroxides (primarily iron, aluminum, calcium, magnesium, manganese, and uranium) and soluble sodium salts (carbonate, hydroxide, nitrite, nitrate, and sulfate). The pretreatment process in the Chemical Processing Cell (CPC) consists of two process tanks, the Sludge Receipt and Adjustment Tank (SRAT) and the Slurry Mix Evaporator (SME) as well as a melter feed tank. During SRAT processing, nitric and formic acids are added to the sludge to lower pH, destroy nitrite and carbonate ions, and reduce mercury and manganese. During the SME cycle, glass formers are added, and the batch is concentrated to the final solids target prior to vitrification. During these processes, hydrogen can be produced by catalytic decomposition of excess formic acid. The waste contains silver, palladium, rhodium, ruthenium, and mercury, but silver and palladium have been shown to be insignificant factors in catalytic hydrogen generation during the DWPF process. A full factorial experimental design was developed to ensure that the existence of statistically significant two-way interactions could be determined without confounding of the main effects with the two-way interaction effects. Rh ranged from 0.0026-0.013% and Ru ranged from 0.010-0.050% in the dried sludge solids, while initial Hg ranged from 0.5-2.5 wt%, as shown in Table 1. The nominal matrix design consisted of twelve SRAT cycles. Testing included: a three factor (Rh, Ru, and Hg) study at two levels per factor (eight runs), three duplicate midpoint runs, and one additional replicate run to assess reproducibility away from the midpoint. Midpoint testing was used to identify potential quadratic effects from the three factors. A single sludge simulant was used for all tests and was spiked with the required amount of noble metals immediately prior to performing the test. Acid addition was kept effectively constant except to compensate for variations in the starting mercury concentration. SME cycles were also performed during six of the tests.

  12. High throughput microcantilever detector

    DOE Patents [OSTI]

    Thundat, Thomas G.; Ferrell, Thomas L.; Hansen, Karolyn M.; Tian, Fang

    2004-07-20

    In an improved uncoated microcantilever detector, the sample sites are placed on a separate semi-conducting substrate and the microcantilever element detects and measures the changes before and after a chemical interaction or hybridization of the sites by sensing differences of phase angle between an alternating voltage applied to the microcantilever element and vibration of the microcantilever element. In another embodiment of the invention, multiple sample sites are on a sample array wherein an array of microcantilever elements detect and measure the change before and after chemical interactions or hybridizations of the sample sites.

  13. High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons

    E-Print Network [OSTI]

    Nieland, Thomas J.

    The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation ...

  14. High Throughput, Low Toxic Processing of Very Thin, High Efficiency CIGSS Solar Cells: Final Report, December 2008

    SciTech Connect (OSTI)

    Dhere, N. G.

    2009-04-01

    The work carried out during this project presents the use of diethylselenium or other organometallic precursors as low-toxicity alternative selenium sources for preparing a high-quality absorber.

  15. Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Slininger, Patricia J; Shea-Andersh, Maureen A; Thompson, Stephanie R; Dien, Bruce S; Kurtzman, Cletus P; Balan, Venkatesh; da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E; Cotta, Michael A

    2015-12-01

    Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment.

  16. Formulation, Pretreatment, and Densification Options to Improve Biomass Specifications for Co-Firing High Percentages with Coal

    SciTech Connect (OSTI)

    Jaya Shankar Tumuluru; J Richard Hess; Richard D. Boardman; Shahab Sokhansanj; Christopher T. Wright; Tyler L. Westover

    2012-06-01

    There is a growing interest internationally to use more biomass for power generation, given the potential for significant environmental benefits and long-term fuel sustainability. However, the use of biomass alone for power generation is subject to serious challenges, such as feedstock supply reliability, quality, and stability, as well as comparative cost, except in situations in which biomass is locally sourced. In most countries, only a limited biomass supply infrastructure exists. Alternatively, co-firing biomass alongwith coal offers several advantages; these include reducing challenges related to biomass quality, buffering the system against insufficient feedstock quantity, and mitigating the costs of adapting existing coal power plants to feed biomass exclusively. There are some technical constraints, such as low heating values, low bulk density, and grindability or size-reduction challenges, as well as higher moisture, volatiles, and ash content, which limit the co-firing ratios in direct and indirect co-firing. To achieve successful co-firing of biomass with coal, biomass feedstock specifications must be established to direct pretreatment options in order to modify biomass materials into a format that is more compatible with coal co-firing. The impacts on particle transport systems, flame stability, pollutant formation, and boiler-tube fouling/corrosion must also be minimized by setting feedstock specifications, which may include developing new feedstock composition by formulation or blending. Some of the issues, like feeding, co-milling, and fouling, can be overcome by pretreatment methods including washing/leaching, steam explosion, hydrothermal carbonization, and torrefaction, and densification methods such as pelletizing and briquetting. Integrating formulation, pretreatment, and densification will help to overcome issues related to physical and chemical composition, storage, and logistics to successfully co-fire higher percentages of biomass ( > 40%) with coal.

  17. High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53 - NF-kappaB - complex-dependent gene expression in human heart failure

    E-Print Network [OSTI]

    Choy, Mun-Kit; Movassagh, Mehregan; Siggens, Lee; Vujic, Ana; Goddard, Martin; Sanchez, Ana; Perkins, Neil; Figg, Nichola; Bennett, Martin; Carroll, Jason; Foo, Roger S.-Y.

    2010-06-14

    with this cis-element. Using high-throughput sequencing, we analyzed genome-wide binding sites for the p53-RELA complex in diseased and control human hearts and found a significant overrepresentation of the STAT3 motif. We further determined that STAT3...

  18. Carbonic Acid Pretreatment of Biomass

    SciTech Connect (OSTI)

    G. Peter van Walsum; Kemantha Jayawardhana; Damon Yourchisin; Robert McWilliams; Vanessa Castleberry

    2003-05-31

    This project sought to address six objectives, outlined below. The objectives were met through the completion of ten tasks. 1) Solidify the theoretical understanding of the binary CO2/H2O system at reaction temperatures and pressures. The thermodynamics of pH prediction have been improved to include a more rigorous treatment of non-ideal gas phases. However it was found that experimental attempts to confirm theoretical pH predictions were still off by a factor of about 1.8 pH units. Arrhenius experiments were carried out and the activation energy for carbonic acid appears to be substantially similar to sulfuric acid. Titration experiments have not yet confirmed or quantified the buffering or acid suppression effects of carbonic acid on biomass. 2) Modify the carbonic acid pretreatment severity function to include the effect of endogenous acid formation and carbonate buffering, if necessary. It was found that the existing severity functions serve adequately to account for endogenous acid production and carbonate effects. 3) Quantify the production of soluble carbohydrates at different reaction conditions and severity. Results show that carbonic acid has little effect on increasing soluble carbohydrate concentrations for pretreated aspen wood, compared to pretreatment with water alone. This appears to be connected to the release of endogenous acids by the substrate. A less acidic substrate such as corn stover would derive benefit from the use of carbonic acid. 4) Quantify the production of microbial inhibitors at selected reaction conditions and severity. It was found that the release of inhibitors was correlated to reaction severity and that carbonic acid did not appear to increase or decrease inhibition compared to pretreatment with water alone. 5) Assess the reactivity to enzymatic hydrolysis of material pretreated at selected reaction conditions and severity. Enzymatic hydrolysis rates increased with severity, but no advantage was detected for the use of carbonic acid compared to water alone. 6) Determine optimal conditions for carbonic acid pretreatment of aspen wood. Optimal severities appeared to be in the mid range tested. ASPEN-Plus modeling and economic analysis of the process indicate that the process could be cost competitive with sulfuric acid if the concentration of solids in the pretreatment is maintained very high (~50%). Lower solids concentrations result in larger reactors that become expensive to construct for high pressure applications.

  19. Abstract--Design aspects of a minimally invasive high-throughput automation system for radiation biodosimetry are

    E-Print Network [OSTI]

    biodosimetry are reported. The system, currently under development, relies on robotic devices and advanced high of the automation system is proposed based on the use of an input stage, a centrifuge module, a cell harvesting

  20. Pretreatment of microbial sludges

    DOE Patents [OSTI]

    Rivard, C.J.; Nagle, N.J.

    1995-01-10

    Methods are described for pretreating microbial sludges to break cells and disrupt organic matter. One method involves the use of sonication, and another method involves the use of shear forces. The pretreatment of sludge enhances bioconversion of the organic fraction. This allows for efficient dewatering of the sludge and reduces the cost for final disposal of the waste.

  1. Pretreatment of microbial sludges

    DOE Patents [OSTI]

    Rivard, Christopher J. (Lakewood, CO); Nagle, Nicholas J. (Louisville, CO)

    1995-01-01

    Methods are described for pretreating microbial sludges to break cells and disrupt organic matter. One method involves the use of sonication, and another method involves the use of shear forces. The pretreatment of sludge enhances bioconversion of the organic fraction. This allows for efficient dewatering of the sludge and reduces the cost for final disposal of the waste.

  2. GREET Pretreatment Module

    SciTech Connect (OSTI)

    Adom, Felix K.; Dunn, Jennifer B.; Han, Jeongwoo

    2014-09-01

    A wide range of biofuels and biochemicals can be produced from biomass via different pretreatment technologies that yield sugars. This report documents the material and energy flows that occur when fermentable sugars from four lignocellulosic feedstocks (corn stover, miscanthus, switchgrass, and poplar) are produced via dilute acid pretreatment and ammonia fiber expansion. These flows are documented for inclusion in the pretreatment module of the Greenhouses Gases, Regulated Emissions, and Energy Use in Transportation (GREET) model. Process simulations of each pretreatment technology were developed in Aspen Plus. Material and energy consumption data from Aspen Plus were then compiled in the GREET pretreatment module. The module estimates the cradle-to-gate fossil energy consumption (FEC) and greenhouse gas (GHG) emissions associated with producing fermentable sugars. This report documents the data and methodology used to develop this module and the cradle-to-gate FEC and GHG emissions that result from producing fermentable sugars.

  3. HIV-1 entry inhibition by small-molecule CCR5 antagonists: A combined molecular modeling and mutant study using a high-throughput assay

    SciTech Connect (OSTI)

    Labrecque, Jean; Metz, Markus; Lau, Gloria; Darkes, Marilyn C.; Wong, Rebecca S.Y.; Bogucki, David; Carpenter, Bryon; Chen Gang; Li Tongshuang; Nan, Susan; Schols, Dominique; Bridger, Gary J.; Fricker, Simon P.; Skerlj, Renato T.

    2011-05-10

    Based on the attrition rate of CCR5 small molecule antagonists in the clinic the discovery and development of next generation antagonists with an improved pharmacology and safety profile is necessary. Herein, we describe a combined molecular modeling, CCR5-mediated cell fusion, and receptor site-directed mutagenesis approach to study the molecular interactions of six structurally diverse compounds (aplaviroc, maraviroc, vicriviroc, TAK-779, SCH-C and a benzyloxycarbonyl-aminopiperidin-1-yl-butane derivative) with CCR5, a coreceptor for CCR5-tropic HIV-1 strains. This is the first study using an antifusogenic assay, a model of the interaction of the gp120 envelope protein with CCR5. This assay avoids the use of radioactivity and HIV infection assays, and can be used in a high throughput mode. The assay was validated by comparison with other established CCR5 assays. Given the hydrophobic nature of the binding pocket several binding models are suggested which could prove useful in the rational drug design of new lead compounds.

  4. Designing and Validating Ternary Pd Alloys for Optimum Sulfur/Carbon Resistance in Hydrogen Separation and Carbon Capture Membrane Systems Using High-Throughput Combinatorial Methods

    SciTech Connect (OSTI)

    Lewis, Amanda; Zhao, Hongbin; Hopkins, Scott

    2014-09-30

    This report summarizes the work completed under the U.S. Department of Energy Project Award No.: DE-FE0001181 titled “Designing and Validating Ternary Pd Alloys for Optimum Sulfur/Carbon Resistance in Hydrogen Separation and Carbon Capture Membrane Systems Using High-Throughput Combinatorial Methods.” The project started in October 1, 2009 and was finished September 30, 2014. Pall Corporation worked with Cornell University to sputter and test palladium-based ternary alloys onto silicon wafers to examine many alloys at once. With the specialized equipment at Georgia Institute of Technology that analyzed the wafers for adsorbed carbon and sulfur species six compositions were identified to have resistance to carbon and sulfur species. These compositions were deposited on Pall AccuSep® supports by Colorado School of Mines and then tested in simulated synthetic coal gas at the Pall Corporation. Two of the six alloys were chosen for further investigations based on their performance. Alloy reproducibility and long-term testing of PdAuAg and PdZrAu provided insight to the ability to manufacture these compositions for testing. PdAuAg is the most promising alloy found in this work based on the fabrication reproducibility and resistance to carbon and sulfur. Although PdZrAu had great initial resistance to carbon and sulfur species, the alloy composition has a very narrow range that hindered testing reproducibility.

  5. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    SciTech Connect (OSTI)

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomics system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.

  6. A case study for cloud based high throughput analysis of NGS data using the globus genomics system

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bhuvaneshwar, Krithika; Sulakhe, Dinanath; Gauba, Robinder; Rodriguez, Alex; Madduri, Ravi; Dave, Utpal; Lacinski, Lukasz; Foster, Ian; Gusev, Yuriy; Madhavan, Subha

    2015-01-01

    Next generation sequencing (NGS) technologies produce massive amounts of data requiring a powerful computational infrastructure, high quality bioinformatics software, and skilled personnel to operate the tools. We present a case study of a practical solution to this data management and analysis challenge that simplifies terabyte scale data handling and provides advanced tools for NGS data analysis. These capabilities are implemented using the “Globus Genomics” system, which is an enhanced Galaxy workflow system made available as a service that offers users the capability to process and transfer data easily, reliably and quickly to address end-to-end NGS analysis requirements. The Globus Genomicsmore »system is built on Amazon's cloud computing infrastructure. The system takes advantage of elastic scaling of compute resources to run multiple workflows in parallel and it also helps meet the scale-out analysis needs of modern translational genomics research.« less

  7. Prediction of rodent carcinogenic potential of naturally occurring chemicals in the human diet using high-throughput QSAR predictive modeling

    SciTech Connect (OSTI)

    Valerio, Luis G. . E-mail: luis.valerio@FDA.HHS.gov; Arvidson, Kirk B.; Chanderbhan, Ronald F.; Contrera, Joseph F.

    2007-07-01

    Consistent with the U.S. Food and Drug Administration (FDA) Critical Path Initiative, predictive toxicology software programs employing quantitative structure-activity relationship (QSAR) models are currently under evaluation for regulatory risk assessment and scientific decision support for highly sensitive endpoints such as carcinogenicity, mutagenicity and reproductive toxicity. At the FDA's Center for Food Safety and Applied Nutrition's Office of Food Additive Safety and the Center for Drug Evaluation and Research's Informatics and Computational Safety Analysis Staff (ICSAS), the use of computational SAR tools for both qualitative and quantitative risk assessment applications are being developed and evaluated. One tool of current interest is MDL-QSAR predictive discriminant analysis modeling of rodent carcinogenicity, which has been previously evaluated for pharmaceutical applications by the FDA ICSAS. The study described in this paper aims to evaluate the utility of this software to estimate the carcinogenic potential of small, organic, naturally occurring chemicals found in the human diet. In addition, a group of 19 known synthetic dietary constituents that were positive in rodent carcinogenicity studies served as a control group. In the test group of naturally occurring chemicals, 101 were found to be suitable for predictive modeling using this software's discriminant analysis modeling approach. Predictions performed on these compounds were compared to published experimental evidence of each compound's carcinogenic potential. Experimental evidence included relevant toxicological studies such as rodent cancer bioassays, rodent anti-carcinogenicity studies, genotoxic studies, and the presence of chemical structural alerts. Statistical indices of predictive performance were calculated to assess the utility of the predictive modeling method. Results revealed good predictive performance using this software's rodent carcinogenicity module of over 1200 chemicals, comprised primarily of pharmaceutical, industrial and some natural products developed under an FDA-MDL cooperative research and development agreement (CRADA). The predictive performance for this group of dietary natural products and the control group was 97% sensitivity and 80% concordance. Specificity was marginal at 53%. This study finds that the in silico QSAR analysis employing this software's rodent carcinogenicity database is capable of identifying the rodent carcinogenic potential of naturally occurring organic molecules found in the human diet with a high degree of sensitivity. It is the first study to demonstrate successful QSAR predictive modeling of naturally occurring carcinogens found in the human diet using an external validation test. Further test validation of this software and expansion of the training data set for dietary chemicals will help to support the future use of such QSAR methods for screening and prioritizing the risk of dietary chemicals when actual animal data are inadequate, equivocal, or absent.

  8. BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments

    SciTech Connect (OSTI)

    Round, Adam, E-mail: around@embl.fr; Felisaz, Franck [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Université Grenoble Alpes–EMBL–CNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Fodinger, Lukas; Gobbo, Alexandre [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Huet, Julien [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Université Grenoble Alpes–EMBL–CNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Villard, Cyril [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Blanchet, Clement E., E-mail: around@embl.fr [EMBL c/o DESY, Notkestrasse 85, 22603 Hamburg (Germany); Pernot, Petra; McSweeney, Sean [ESRF, 6 Rue Jules Horowitz, 38000 Grenoble (France); Roessle, Manfred; Svergun, Dmitri I. [EMBL c/o DESY, Notkestrasse 85, 22603 Hamburg (Germany); Cipriani, Florent, E-mail: around@embl.fr [European Molecular Biology Laboratory, Grenoble Outstation, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France); Université Grenoble Alpes–EMBL–CNRS, 71 Avenue des Martyrs, CS 90181, 38042 Grenoble (France)

    2015-01-01

    A robotic sample changer for solution X-ray scattering experiments optimized for speed and to use the minimum amount of material has been developed. This system is now in routine use at three high-brilliance European synchrotron sites, each capable of several hundred measurements per day. Small-angle X-ray scattering (SAXS) of macromolecules in solution is in increasing demand by an ever more diverse research community, both academic and industrial. To better serve user needs, and to allow automated and high-throughput operation, a sample changer (BioSAXS Sample Changer) that is able to perform unattended measurements of up to several hundred samples per day has been developed. The Sample Changer is able to handle and expose sample volumes of down to 5 µl with a measurement/cleaning cycle of under 1 min. The samples are stored in standard 96-well plates and the data are collected in a vacuum-mounted capillary with automated positioning of the solution in the X-ray beam. Fast and efficient capillary cleaning avoids cross-contamination and ensures reproducibility of the measurements. Independent temperature control for the well storage and for the measurement capillary allows the samples to be kept cool while still collecting data at physiological temperatures. The Sample Changer has been installed at three major third-generation synchrotrons: on the BM29 beamline at the European Synchrotron Radiation Facility (ESRF), the P12 beamline at the PETRA-III synchrotron (EMBL@PETRA-III) and the I22/B21 beamlines at Diamond Light Source, with the latter being the first commercial unit supplied by Bruker ASC.

  9. Hazard Analysis for the Pretreatment Engineering Platform (PEP)

    SciTech Connect (OSTI)

    Sullivan, Robin S.; Geeting, John GH; Lawrence, Wesley E.; Young, Jonathan

    2008-07-10

    The Pretreatment Engineering Platform (PEP) is designed to perform a demonstration on an engineering scale to confirm the Hanford Waste Treatment Plant Pretreatment Facility (PTF) leaching and filtration process equipment design and sludge treatment process. The system will use scaled prototypic equipment to demonstrate sludge water wash, caustic leaching, oxidative leaching, and filtration. Unit operations to be tested include pumping, solids washing, chemical reagent addition and blending, heating, cooling, leaching, filtration, and filter cleaning. In addition, the PEP will evaluate potential design changes to the ultrafiltration process system equipment to potentially enhance leaching and filtration performance as well as overall pretreatment throughput. The skid-mounted system will be installed and operated in the Processing Development Laboratory-West at Pacific Northwest National Laboratory (PNNL) in Richland, Washington.

  10. High-Throughput Plasmonic Nanolithography

    E-Print Network [OSTI]

    Pan, Liang

    2010-01-01

    60 4.2.5 Lubricant and Overcoats63 Figure 4.10: Scheme of lubricant durability65 Figure 4.11: Three lubricant flying tests at different

  11. High-Throughput Plasmonic Nanolithography

    E-Print Network [OSTI]

    Pan, Liang

    2010-01-01

    heating Module..FH Control and Resist Pre-heating Module Inspired by thecontrol and resist pre-heating module using the same laser

  12. Biochemical & Thermochemical High Throughput Characterization...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    20 40 60 80 100 120 140 32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62 64 66 68 70 Frequency Corn Stover Corn Cob Miscanthus Wheat...

  13. Combinatorial Chemistry & High Throughput Screening, 2001, 4, 661-673 661 AnEvolutionaryApproachfor Structure-based Design of Natural and Non-

    E-Print Network [OSTI]

    Caflisch, Amedeo

    EvolutionaryApproachfor Structure-based Design of Natural and Non- natural Peptidic Ligands Nicolas Budin, Shaheen Ahmed, Nicolas- throughput homology modelling [4] are providing a large amount of targets for structure-based drug design-8057 Zürich, Switzerland Abstract: A new computational approach (PEP) is presented for the structure

  14. 2011 DoD High Performance Computing Modernization Program Users Group Conference A Web-based High-Throughput Tool for Next-Generation Sequence Annotation

    E-Print Network [OSTI]

    320 2011 DoD High Performance Computing Modernization Program Users Group Conference A Web deployed on the Mana Linux cluster at the Maui High Performance Computing Center. The two components

  15. SIPS: A small modular process unit for the in-tank pretreatment of high-level wastes

    SciTech Connect (OSTI)

    Reich, M.; Powell, J.; Barletta, R. [Brookhaven National Lab., Upton, NY (United States)

    1996-12-31

    As a result of the U.S. weapons production program, there are now hundreds of large tanks containing highly radioactive wastes. Safe disposal of these wastes requires their processing and separations into a small volume of highly radioactive waste (HLW) and a much larger volume of low-level waste (LLW). The HLW waste would then be vitrified and transported to a geologic repository. To date, the principal approach proposed for the separation envisions a large, centralized process facility. The small in-tank processing system (SIPS) is a proposed new, small modular concept for the in-tank processing and separation of wastes into HLW and LLW output streams suitable for vitrification. Instead of pumping the retrieved tank wastes as a solid/liquid slurry over long distances to a centralized process facility, SIPS would employ a small process module, typically {approximately}1 m in diameter and 4 m long, which would be inserted into the tank. Over a period of {approx} 6 months, the module would process the solid/liquid materials in the tank, producing separated liquid HLW and liquid LLW output streams that are pumped away in two small-diameter ({approx}3-cm outside diameter) pipes. The SIPS module would be serviced by five auxiliary small pipes - a water feed pipe, a water feed pipe containing micron-size ferromagnetic particles, a nitric acid ({approx}3 M) feed pipe, and input/out pipes to hydraulically load/unload ion exchange beads.

  16. Throughput of proportional fair scheduling over Rayleigh fading channels

    E-Print Network [OSTI]

    Leung, Kin K.

    Throughput of proportional fair scheduling over Rayleigh fading channels E. Liu and K.K. Leung The proportional fair scheduling (PFS) algorithm is implemented in current 3G wireless networks for high data rate of proportional fairness is NP-hard. An analytical expression is obtained to approximate the throughput of PFS

  17. Biomass shock pretreatment

    DOE Patents [OSTI]

    Holtzapple, Mark T.; Madison, Maxine Jones; Ramirez, Rocio Sierra; Deimund, Mark A.; Falls, Matthew; Dunkelman, John J.

    2014-07-01

    Methods and apparatus for treating biomass that may include introducing a biomass to a chamber; exposing the biomass in the chamber to a shock event to produce a shocked biomass; and transferring the shocked biomass from the chamber. In some aspects, the method may include pretreating the biomass with a chemical before introducing the biomass to the chamber and/or after transferring shocked biomass from the chamber.

  18. Vertical Pretreatment Reactor System (Poster)

    SciTech Connect (OSTI)

    Not Available

    2012-09-01

    IBRF poster developed for the IBRF showcase. Describes the two-vessel system for primary and secondary pretreatment of biomass solids at different temperatures.

  19. PROJECT W-551 INTERIM PRETREATMENT SYSTEM PRECONCEPTUAL CANDIDATE TECHNOLOGY DESCRIPTIONS

    SciTech Connect (OSTI)

    MAY TH

    2008-08-12

    The Office of River Protection (ORP) has authorized a study to recommend and select options for interim pretreatment of tank waste and support Waste Treatment Plant (WTP) low activity waste (LAW) operations prior to startup of all the WTP facilities. The Interim Pretreatment System (IPS) is to be a moderately sized system which separates entrained solids and 137Cs from tank waste for an interim time period while WTP high level waste vitrification and pretreatment facilities are completed. This study's objective is to prepare pre-conceptual technology descriptions that expand the technical detail for selected solid and cesium separation technologies. This revision includes information on additional feed tanks.

  20. The fate of lignin during hydrothermal pretreatment

    E-Print Network [OSTI]

    Trajano, Heather L; Engle, Nancy L; Foston, Marcus; Ragauskas, Arthur J; Tschaplinski, Timothy J; Wyman, Charles E

    2013-01-01

    straw pretreated for bioethanol production. Biotechnologyof woody biomass for bioethanol production. Bioresources

  1. The fate of lignin during hydrothermal pretreatment

    E-Print Network [OSTI]

    Trajano, Heather L; Engle, Nancy L; Foston, Marcus; Ragauskas, Arthur J; Tschaplinski, Timothy J; Wyman, Charles E

    2013-01-01

    pretreated for bioethanol production. Biotechnology forwoody biomass for bioethanol production. Bioresources 2011,

  2. Processes for pretreating lignocellulosic biomass: A review

    SciTech Connect (OSTI)

    McMillan, J.D.

    1992-11-01

    This paper reviews existing and proposed pretreatment processes for biomass. The focus is on the mechanisms by which the various pretreatments act and the influence of biomass structure and composition on the efficacy of particular pretreatment techniques. This analysis is used to identify pretreatment technologies and issues that warrant further research.

  3. Exposure Based Health Issues Project Report: Phase I of High Level Tank Operations, Retrieval, Pretreatment, and Vitrification Exposure Based Health Issues Analysis

    SciTech Connect (OSTI)

    Stenner, Robert D.; Bowers, Harold N.; Kenoyer, Judson L.; Strenge, Dennis L.; Brady, William H.; Ladue, Buffi; Samuels, Joseph K.

    2001-11-30

    The Department of Energy (DOE) has the responsibility to understand the ''big picture'' of worker health and safety which includes fully recognizing the vulnerabilities and associated programs necessary to protect workers at the various DOE sites across the complex. Exposure analysis and medical surveillance are key aspects for understanding this big picture, as is understanding current health and safety practices and how they may need to change to relate to future health and safety management needs. The exposure-based health issues project was initiated to assemble the components necessary to understand potential exposure situations and their medical surveillance and clinical aspects. Phase I focused only on current Hanford tank farm operations and serves as a starting point for the overall project. It is also anticipated that once the pilot is fully developed for Hanford HLW (i.e., current operations, retrieval, pretreatment, vitrification, and disposal), the process and analysis methods developed will be available and applicable for other DOE operations and sites. The purpose of this Phase I project report is to present the health impact information collected regarding ongoing tank waste maintenance operations, show the various aspects of health and safety involved in protecting workers, introduce the reader to the kinds of information that will need to be analyzed in order to effectively manage worker safety.

  4. Efficient Algebraic Representations for Throughput-Oriented Algorithms

    E-Print Network [OSTI]

    McKinlay, Christopher E.

    2013-01-01

    Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . . .Throughput-Oriented Algorithm Design Multilinear

  5. Delay and throughput analysis of tree algorithms for random access over noisy collision channels

    E-Print Network [OSTI]

    /FS was also combined with the binary exponential backoff (BEB) algorithm in [5] in an 802.16 setting of the BEB algorithm. Furthermore, [5] also indicated that the saturation throughput of SICTA/FS degrades quickly as the number of users increases, while BEB-SICTA/FS still achieves high throughputs

  6. High throughput liquid absorption preconcentrator sampling instrument

    DOE Patents [OSTI]

    Zaromb, S.; Bozen, R.M.

    1992-12-22

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis. 12 figs.

  7. High throughput solar cell ablation system

    DOE Patents [OSTI]

    Harley, Gabriel; Pass, Thomas; Cousins, Peter John; Viatella, John

    2014-10-14

    A solar cell is formed using a solar cell ablation system. The ablation system includes a single laser source and several laser scanners. The laser scanners include a master laser scanner, with the rest of the laser scanners being slaved to the master laser scanner. A laser beam from the laser source is split into several laser beams, with the laser beams being scanned onto corresponding wafers using the laser scanners in accordance with one or more patterns. The laser beams may be scanned on the wafers using the same or different power levels of the laser source.

  8. High throughput solar cell ablation system

    DOE Patents [OSTI]

    Harley, Gabriel; Pass, Thomas; Cousins, Peter John; Viatella, John

    2012-09-11

    A solar cell is formed using a solar cell ablation system. The ablation system includes a single laser source and several laser scanners. The laser scanners include a master laser scanner, with the rest of the laser scanners being slaved to the master laser scanner. A laser beam from the laser source is split into several laser beams, with the laser beams being scanned onto corresponding wafers using the laser scanners in accordance with one or more patterns. The laser beams may be scanned on the wafers using the same or different power levels of the laser source.

  9. A toolkit for high-throughput, cross-

    E-Print Network [OSTI]

    Cai, Long

    , Mihail Sarov, Sylke Winkler, Kamil A Lipinski & Pavel Tomancak We generated two complementary genomic. pseudoobscurab 88% 12% 0.12% 8.25% 3.65% Mapped clones (15,204 D.mel / 1,924 D.pse) Sequencing failures (1,426 D.mel / 142 D.pse) BLAST failures (20 D.mel / 1 D.pse) Chimeric clones (530 D.mel / 525 D.pse) 74% 26% 0.04% 5

  10. High Throughput Materials Characterization John M. Gregoire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenry Bellamy, Ph.D. Title: Professor -|

  11. Ensemble Jobs for Better Throughput - Videoconference | Argonne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ensemble Jobs for Better Throughput - Videoconference Event Sponsor: Argonne National Laboratory Start Date: Sep 24 2015 - 1:00pm BuildingRoom: Online Videoconference Location:...

  12. Pre-treating Seed to Enhance Germination of Desert Shrubs

    SciTech Connect (OSTI)

    W. K. Ostler; D. C. Anderson; D. J. Hansen

    2002-06-01

    Creosotebush [Larrea tridentata (D.C.) Cav.] and white bursage [Ambrosia dumosa (A. Gray) W.W. Payne] seeds were subjected to pre-treatments of rinsing and soaking in water and thiourea to enhance germination in laboratory experiments. The effects of darkness, temperature, seed source, and soil moisture were also evaluated in the laboratory. The best pre-treatment from the laboratory experiments, rinsing with water for 36 hours followed by drying, was field-tested at Fort Irwin, California. Two sites and two seeding dates (early March and mid April) were determined for each site. Five mulch treatments (no mulch, straw, gravel, chemical stabilizer, and plastic) were evaluated in combination with the seed pre-treatments. Field emergence was greatly enhanced with the seed pre-treatment for white bursage during the March (18-42% increase in germination) and April seedings (16-23% increase in germination). Creosotebush showed poor germination during March (2-5%) when soil temperatures averaged 15 C, but germination increased during the April trials (6-43%) when soil temperatures averaged 23 C. The seed pre-treatment during the April trials increased germination from 16-23%. The plastic mulch treatment increased germination dramatically during both the March and April trials. The plastic mulch increased soil temperatures (8-10 C)and maintained high humidity during germination. Both the chemical stabilizer and the gravel mulches improved germination over the control while the straw mulch decreased germination. These results suggest that seed pre-treatments combined with irrigation and mulch are effective techniques to establish these two dominant Mojave Desert species from seed.

  13. On Achieving Optimal Throughput with Network Coding

    E-Print Network [OSTI]

    Li, Baochun

    to the problem of optimizing end-to- end throughput in undirected data networks. We first illustrate the powerOn Achieving Optimal Throughput with Network Coding Zongpeng Li, Baochun Li, Dan Jiang, Lap Chi Lau Abstract-- With the constraints of network topologies and link capacities, achieving the optimal end

  14. Effects of pretreatment in steam on the pyrolysis behavior of Loy Yang brown coal

    SciTech Connect (OSTI)

    Cai Zeng; George Favas; Hongwei Wu; Alan L. Chaffee; Jun-ichiro Hayashi; Chun-Zhu Li [Monash University, Vic. (Australia). CRC for Clean Power from Lignite, Department of Chemical Engineering

    2006-02-01

    Dewatering/drying of Victorian brown coal will be an integral part of future brown coal utilization processes aimed at the reduction of greenhouse gas emissions. This study aims to investigate the effects of the thermal pretreatment of brown coal in the presence of steam/water on its subsequent pyrolysis behavior. A Victorian (Loy Yang) brown coal was thermally pretreated in pressurized steam and inert atmospheres. The pyrolysis behavior of these pretreated coal samples was investigated in a wire-mesh reactor. While the pretreatment in steam at temperatures higher than 250{sup o}C increased the char yield of the steam-treated coal, it did not affect the overall pyrolysis char yield at 1000{sup o} C s{sup -1} if the weight loss during the pretreatment in steam was also considered. However, the tar yield decreased significantly after the pretreatment in the presence of steam. The UV-fluorescence spectroscopy of tars revealed that the release of large aromatic systems from the steam-treated coal was only affected by the pretreatment in steam if the treatment temperature was very high (e.g. 350{sup o}C). The loss of NaCl and the use of high pressure during the pretreatment of brown coal in steam were not the main reasons for the changes in the observed tar yield. The hydrolysis of O-containing structures such as ethers, esters, and carboxylates during the pretreatment in the presence of steam plays an important role in the fates of these O-containing structures during pretreatment and subsequent pyrolysis, leading to changes in the pyrolysis behavior of the brown coal. 36 refs., 8 figs.

  15. Optimization of steam explosion pretreatment. Final report

    SciTech Connect (OSTI)

    Foody, P.

    1980-04-01

    Different operating conditions are required to optimize the yield from each of the various fractions in the substrate. Xylose recovery is maximized at short cooking times whereas maximum lignin recovery requires much longer cooking times. Peak glucose yield and rumen digestibility occur at intermediate times. If process conditions are set for maximum glucose yield we have achieved a yield of 68% of the theoretical, based on an average of a dozen substrates tested. Individual results ranged from 46 to 87%. If the process is optimized for maximum total sugars (i.e. glucose plus xylose) we have obtained an average yield of 60%, with a range of 31 to 75%. With rumen microflora, the average value of the in-vitro cellulose digestibility was 82%, with a range of 41 to 90%. The optimum operating conditions for total sugars are a pressure of 500 to 550 psig with a cooking time of 40 to 50 seconds and 35% starting moisture content. Particle size is not a significant factor, nor is pre-steaming or use of a constricting die in the gun nozzle. High quality lignin can be extracted with 80% yield. The Iotech lignin is very soluble, has a low molecular weight and is reactive. The unique properties of the lignin derive from the explosion at the end of the pretreatment. A lignin formaldehyde resin has been successfully formulated and tested. It represents a high value utilization of the lignin byproduct with immediate market potential. A detailed engineering design of the process gives an estimated operating cost of $7.50/OD ton of biomass. At this low cost, the Iotech process achieves many important pretreatment goals in a single step. The substrate has been sterilized; it has been pulverized into a powder; the cellulose has been accessible; and a highly reactive lignin fraction can be recovered and utilized.

  16. Computer simulation and economic analysis for ammonia fiber explosion (AFEX) pretreatment process 

    E-Print Network [OSTI]

    Wang, Lin

    1996-01-01

    The ammonia fiber explosion (AFFECT) process is a promising new pretreatment for enhancing the reactivity of lignocerulose materials with many advantages over existing processes. The material is soaked in high-pressure ...

  17. Waste Treatment and Immobilation Plant Pretreatment Facility

    Office of Environmental Management (EM)

    7 Technology Readiness Assessment for the Waste Treatment and Immobilization Plant (WTP) Pretreatment Facility L. Holton D. Alexander M. Johnson H. Sutter August 2007 Prepared by...

  18. Ionic Liquid Pretreatment Process for Biomass Is Successfully...

    Energy Savers [EERE]

    Ionic Liquid Pretreatment Process for Biomass Is Successfully Implemented at Larger Scale Ionic Liquid Pretreatment Process for Biomass Is Successfully Implemented at Larger Scale...

  19. Pretreatment of neutralized cladding removal waste sludge: Status Report

    SciTech Connect (OSTI)

    Lumetta, G J; Swanson, J L

    1993-03-01

    This report describes the status of process development for pretreating Hanford neutralized cladding removal waste (NCRW) sludge, of which [approximately] 3.3 [times] 10[sup 6] L is stored in Tanks 103-AW and 105-AW at the Hanford Site. The initial baseline process chosen for pretreating NCRW sludge is to dissolve the sludge in nitric acid and extract the -transuranic (MU) elements from the dissolved sludge solution with octyl(phenyl)-N,N-diisobutylcarbamoyl methyl phosphine oxide (CNWO). This process converts the NCRW sludge into a relatively large volume of low-level waste (LLW) to be disposed of as grout, leaving only a small volume of high-level waste (HLW) requiring vitrification in the Hanford Waste Vitrification Plant (HWVP).

  20. Description of waste pretreatment and interfacing systems dynamic simulation model

    SciTech Connect (OSTI)

    Garbrick, D.J.; Zimmerman, B.D.

    1995-05-01

    The Waste Pretreatment and Interfacing Systems Dynamic Simulation Model was created to investigate the required pretreatment facility processing rates for both high level and low level waste so that the vitrification of tank waste can be completed according to the milestones defined in the Tri-Party Agreement (TPA). In order to achieve this objective, the processes upstream and downstream of the pretreatment facilities must also be included. The simulation model starts with retrieval of tank waste and ends with vitrification for both low level and high level wastes. This report describes the results of three simulation cases: one based on suggested average facility processing rates, one with facility rates determined so that approximately 6 new DSTs are required, and one with facility rates determined so that approximately no new DSTs are required. It appears, based on the simulation results, that reasonable facility processing rates can be selected so that no new DSTs are required by the TWRS program. However, this conclusion must be viewed with respect to the modeling assumptions, described in detail in the report. Also included in the report, in an appendix, are results of two sensitivity cases: one with glass plant water recycle steams recycled versus not recycled, and one employing the TPA SST retrieval schedule versus a more uniform SST retrieval schedule. Both recycling and retrieval schedule appear to have a significant impact on overall tank usage.

  1. Stable Throughput in a Cognitive Wireless Network

    E-Print Network [OSTI]

    Fanous, Anthony

    2012-01-01

    We study, from a network layer perspective, the effect of an Ad-Hoc secondary network with N nodes randomly accessing the spectrum licensed to a primary node during the idle slots of the primary user. If the sensing is perfect, then the secondary nodes do not interfere with the primary node and hence do not affect its stable throughput. In case of imperfect sensing, it is shown that if the primary user's arrival rate is less than some calculated finite value, cognitive nodes can employ any transmission power or probabilities without affecting the primary user's stability; otherwise, the secondary nodes should control their transmission parameters to reduce the interference on the primary. It is also shown that in contrast with the primary's maximum stable throughput which strictly decreases with increased sensing errors, the throughput of the secondary nodes might increase with sensing errors as more transmission opportunities become available to them. Finally, we explore the use of the secondary nodes as rel...

  2. Determination of saccharides and ethanol from biomass conversion using Raman spectroscopy: Effects of pretreatment and enzyme composition

    SciTech Connect (OSTI)

    Shih, Chien-Ju

    2010-05-16

    This dissertation focuses on the development of facile and rapid quantitative Raman spectroscopy measurements for the determination of conversion products in producing bioethanol from corn stover. Raman spectroscopy was chosen to determine glucose, xylose and ethanol in complex hydrolysis and fermentation matrices. Chapter 1 describes the motives and main goals of this work, and includes an introduction to biomass, commonly used pretreatment methods, hydrolysis and fermentation reactions. The principles of Raman spectroscopy, its advantages and applications related to biomass analysis are also illustrated. Chapter 2 and 3 comprise two published or submitted manuscripts, and the thesis concludes with an appendix. In Chapter 2, a Raman spectroscopic protocol is described to study the efficiency of enzymatic hydrolysis of cellulose by measuring the main product in hydrolysate, glucose. Two commonly utilized pretreatment methods were investigated in order to understand their effect on glucose measurements by Raman spectroscopy. Second, a similar method was set up to determine the concentration of ethanol in fermentation broth. Both of these measurements are challenged by the presence of complex matrices. In Chapter 3, a quantitative comparison of pretreatment protocols and the effect of enzyme composition are studied using systematic methods. A multipeak fitting algorithm was developed to analyze spectra of hydrolysate containing two analytes: glucose and xylose. Chapter 4 concludes with a future perspective of this research area. An appendix describes a convenient, rapid spectrophotometric method developed to measure cadmium in water. This method requires relatively low cost instrumentation and can be used in microgravity, such as space shuttles or the International Space Station. This work was performed under the supervision of Professor Marc Porter while at Iowa State University. Research related to producing biofuel from bio-renewable resources, especially bioethanol from biomass, has grown significantly in the past decade due to the high demand and rising costs of fossil fuels. More than 3 percent of the energy consumption in the U.S. is derived from renewable biomass, mostly through industrial heat and steam production by the pulp and paper industry, and electricity generation from municipal solid waste (MSW) and forest industry residues. The utilization of food-based biomass to make fuels has been widely criticized because it may increase food shortages throughout the world and raise the cost of food. Thus, nonfood-based and plentiful lignocellulosic feedstocks, such as corn stover, perennial grass, bagasse, sorghum, wheat/rice straw, herbaceous and woody crops, have great potential to be new bio-renewable sources for energy production. Given that many varieties of biomass are available, there is need for a rapid, simple, high-throughput method to screen the conversion of many plant varieties. The most suitable species for each geographic region must be determined, as well as the optimal stage of harvest, impacts of environmental conditions (temperature, soil, pH, etc.). Various genetically modified plants should be studied in order to establish the desired biomass in bioethanol production. The main screening challenge, however, is the complexity of plant cell wall structures that make reliable and sensitive analysis difficult. To date, one of the most popular methods to produce lignocellulosic ethanol is to perform enzymatic hydrolysis followed by fermentation of the hydrolysate with yeast. There are several vital needs related to the field of chemistry that have been suggested as primary research foci needed to effectively improve lignocellulosic ethanol production. These topics include overcoming the recalcitrance of cellulosic biomass, the pervasiveness of pretreatment, advanced biological processing and better feedstocks. In this thesis, a novel approach using Raman spectroscopy has been developed to address important issues related to bioethanol generation, which will aid the research aimed to solve the topics m

  3. An overlay architecture for throughput optimal multipath routing

    E-Print Network [OSTI]

    Jones, Nathaniel M.

    Legacy networks are often designed to operate with simple single-path routing, like shortest-path, which is known to be throughput suboptimal. On the other hand, previously proposed throughput optimal policies (i.e., ...

  4. Throughput Analysis of Wireless Ad-Hoc Cognitive Radio Networks 

    E-Print Network [OSTI]

    Banaei, Armin

    2014-04-25

    In this dissertation we consider the throughput performance of cognitive radio networks and derive the optimal sensing and access schemes for secondary users that maximizes their sum-throughput while guaranteeing certain ...

  5. Final Report - "Foaming and Antifoaming and Gas Entrainment in Radioactive Waste Pretreatment and Immobilization Processes"

    SciTech Connect (OSTI)

    Wasan, Darsh T.

    2007-10-09

    The Savannah River Site (SRS) and Hanford site are in the process of stabilizing millions of gallons of radioactive waste slurries remaining from production of nuclear materials for the Department of Energy (DOE). The Defense Waste Processing Facility (DWPF) at SRS is currently vitrifying the waste in borosilicate glass, while the facilities at the Hanford site are in the construction phase. Both processes utilize slurry-fed joule-heated melters to vitrify the waste slurries. The DWPF has experienced difficulty during operations. The cause of the operational problems has been attributed to foaming, gas entrainment and the rheological properties of the process slurries. The rheological properties of the waste slurries limit the total solids content that can be processed by the remote equipment during the pretreatment and meter feed processes. Highly viscous material can lead to air entrainment during agitation and difficulties with pump operations. Excessive foaming in waste evaporators can cause carryover of radionuclides and non-radioactive waste to the condensate system. Experimental and theoretical investigations of the surface phenomena, suspension rheology and bubble generation of interactions that lead to foaming and air entrainment problems in the DOE High Level and Low Activity Radioactive Waste separation and immobilization processes were pursued under this project. The first major task accomplished in the grant proposal involved development of a theoretical model of the phenomenon of foaming in a three-phase gas-liquid-solid slurry system. This work was presented in a recently completed Ph.D. thesis (9). The second major task involved the investigation of the inter-particle interaction and microstructure formation in a model slurry by the batch sedimentation method. Both experiments and modeling studies were carried out. The results were presented in a recently completed Ph.D. thesis. The third task involved the use of laser confocal microscopy to study the effectiveness of three slurry rheology modifiers. An effective modifier was identified which resulted in lowering the yield stress of the waste simulant. Therefore, the results of this research have led to the basic understanding of the foaming/antifoaming mechanism in waste slurries as well as identification of a rheology modifier, which enhances the processing throughput, and accelerates the DOE mission. The objectives of this research effort were to develop a fundamental understanding of the physico-chemical mechanisms that produced foaming and air entrainment in the DOE High Level (HLW) and Low Activity (LAW) radioactive waste separation and immobilization processes, and to develop and test advanced antifoam/defoaming/rheology modifier agents. Antifoams/rheology modifiers developed from this research ere tested using non-radioactive simulants of the radioactive wastes obtained from Hanford and the Savannah River Site (SRS).

  6. Throughput Maximization in Wireless Powered Communication Networks with Energy Saving

    E-Print Network [OSTI]

    Brown III, Donald R.

    Throughput Maximization in Wireless Powered Communication Networks with Energy Saving Rui Wang, D and energy allocation to maximize the sum throughput for the case when the nodes can save energy for later with energy saving provides improved sum throughput increasing with the number of transmission blocks. Index

  7. Methods of hydrolyzing pretreated densified biomass particulates and systems related thereto

    SciTech Connect (OSTI)

    Bals, Bryan; Teymouri, Farzaneh; Campbell, Tim; Dale, Bruce

    2015-02-03

    A method is provided in which pretreated and densified cellulosic biomass particulates can be hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or an entire suite of other useful bioproducts.

  8. Effect of pelleting on the recalcitrance and bioconversion of dilute-acid pretreated corn stover

    SciTech Connect (OSTI)

    Allison E Ray; Amber Hoover; Gary Gresham

    2012-07-01

    Background: Knowledge regarding the performance of densified biomass in biochemical processes is limited. The effects of densification on biochemical conversion are explored here. Methods: Pelleted corn stover samples were generated from bales that were milled to 6.35 mm. Low-solids acid pretreatment and simultaneous saccharification and fermentation were performed to evaluate pretreatment efficacy and ethanol yields achieved for pelleted and ground stover (6.35 mm and 2 mm) samples. Both pelleted and 6.35-mm ground stover were evaluated using a ZipperClave® reactor under high-solids, process-relevant conditions for multiple pretreatment severities (Ro), followed by enzymatic hydrolysis of the washed, pretreated solids. Results: Monomeric xylose yields were significantly higher for pellets (approximately 60%) than for ground formats (approximately 38%). Pellets achieved approximately 84% of theoretical ethanol yield (TEY); ground stover formats had similar profiles, reaching approximately 68% TEY. Pelleting corn stover was not detrimental to pretreatment efficacy for both low- and high-solids conditions, and even enhanced ethanol yields.

  9. Investigations of Biomass Pretreatment and Submerged Fixed-bed Fermentation 

    E-Print Network [OSTI]

    Meysing, Daniel

    2012-02-14

    To improve the MixAlco process and biomass pretreatment, five studies were conducted. Three studies related to fermentation, whereas the other two investigated the effectiveness of shock tube pretreatment (STP) coupled ...

  10. Pretreatment and Process Hydrolysis Presentation for BETO 2015...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    effective upgrading * Utilize all carbon * Increase rural employment opportunities * Lower petroleum imports * Decrease carbon footprint. 2 Project Overview Pretreatment and...

  11. New hydrocracking catalysts increase throughput, run length

    SciTech Connect (OSTI)

    Huizinga, T. [Shell Internationale Petroleum Mij., The Hague (Netherlands); Theunissen, J.M.H. [Rayong Refinery Co. Ltd., Rayong (Thailand); Minderhoud, H.; Veen, R. van [Koninklijke/Shell-Lab., Amsterdam (Netherlands)

    1995-06-26

    An improved, second-stage hydrocracking catalyst has been developed by combining stabilized Y zeolites with amorphous silica alumina cracking components. A commercial application of this catalyst, along with a new, first-stage zeolitic hydrocracking catalyst, resulted in increased unit throughput and cycle length. The paper discusses the hydrocracking process, first-stage catalysts, second-stage catalysts, hydrogenation process, commercial results, and product properties.

  12. Parboil: A Revised Benchmark Suite for Scientific and Commercial Throughput Computing

    E-Print Network [OSTI]

    Hwu, Wen-mei W.

    are presented as challenges to the compiler and architecture research communities: to develop the technology Technical Report IMPACT-12-01 University of Illinois at Urbana-Champaign Center for Reliable and High computing applications useful for studying the performance of throughput computing architecture

  13. Stealthy Traffic Analysis of Low-Latency Anonymous Communication Using Throughput Fingerprinting

    E-Print Network [OSTI]

    Singh, Jaswinder Pal

    such as Tor aim to enable users to com- municate in a manner that is untraceable by adversaries that control this leaks in- formation about the set of Tor relays in a circuit (path). We present attacks that, with high the bottleneck relay of any Tor circuit whose throughput can be observed, (b) exactly identify the guard relay

  14. Pretreatment process for forming a smooth surface diamond film on a carbon-coated substrate

    DOE Patents [OSTI]

    Feng, Z.; Brewer, M.; Brown, I.; Komvopoulos, K.

    1994-05-03

    A process is disclosed for the pretreatment of a carbon-coated substrate to provide a uniform high density of nucleation sites thereon for the subsequent deposition of a continuous diamond film without the application of a bias voltage to the substrate. The process comprises exposing the carbon-coated substrate, in a microwave plasma enhanced chemical vapor deposition system, to a mixture of hydrogen-methane gases, having a methane gas concentration of at least about 4% (as measured by partial pressure), while maintaining the substrate at a pressure of about 10 to about 30 Torr during the pretreatment. 6 figures.

  15. Pretreatment process for forming a smooth surface diamond film on a carbon-coated substrate

    DOE Patents [OSTI]

    Feng, Zhu (Albany, CA); Brewer, Marilee (Goleta, CA); Brown, Ian (Berkeley, CA); Komvopoulos, Kyriakos (Orinda, CA)

    1994-01-01

    A process is disclosed for the pretreatment of a carbon-coated substrate to provide a uniform high density of nucleation sites thereon for the subsequent deposition of a continuous diamond film without the application of a bias voltage to the substrate. The process comprises exposing the carbon-coated substrate, in a microwave plasma enhanced chemical vapor deposition system, to a mixture of hydrogen-methane gases, having a methane gas concentration of at least about 4% (as measured by partial pressure), while maintaining the substrate at a pressure of about 10 to about 30 Torr during the pretreatment.

  16. PROJECT W-551 SUMMARY INFORMATION FOR EARLY LAW INTERIM PRETREATMENT SYSTEM SELECTION

    SciTech Connect (OSTI)

    TEDESCHI AR

    2008-08-11

    This report provides summary data for use by the decision board to assess and select the final technology for project W-551, Interim Pretreatment System. This project will provide early pretreated low activity waste feed to the Waste Treatment Plant to allow Waste Treatment Plan Low Activity Waste facility operation prior to construction completion of the Pretreatment and High Level Waste facilities. The candidate solids separations technologies are rotary microfiltration and crossflow filtration, and the candidate cesium separation technologies are fractional crystallization, caustic-side solvent extraction, and ion-exchange using spherical resorcinol-fonnaldebyde resin. This document provides a summary of comparative data against prior weighted criteria to support technology selection. Supporting details and background for this summary are documented in the separate report, RPP-RPT-37741.

  17. PROJECT W-551 DETERMINATION DATA FOR EARLY LAW INTERIM PRETREATMENT SYSTEM SELECTION

    SciTech Connect (OSTI)

    TEDESCHI AR

    2008-08-11

    This report provides the detailed assessment forms and data for selection of the solids separation and cesium separation technology for project W-551, Interim Pretreatment System. This project will provide early pretreated low activity waste feed to the Waste Treatment Plant to allow Waste Treatment Plan Low Activity Waste facility operation prior to construction completion of the Pretreatment and High Level Waste facilities. The candidate solids separations technologies are rotary microfiltration and crossflow filtration, and the candidate cesium separation technologies are fractional crystallization, caustic-side solvent extraction, and ion-exchange using spherical resorcinol-formaldehyde resin. This data was used to prepare a cross-cutting technology summary, reported in RPP-RPT-37740.

  18. Comparative Sugar Recovery and Fermentation Data Following Pretreatment of Poplar Wood by Leading Technologies

    E-Print Network [OSTI]

    California at Riverside, University of

    , aqueous ammonia recycle, dilute sulfuric acid, lime, neu- tral pH, and sulfur dioxide were applied pretreatments to ethanol with similarly high yields. An Agricultural and Industrial Advisory Board followed of Chemical Engineers Biotechnol. Prog., 25: 333­339, 2009 Keywords: ammonia fiber expansion, ammonia recycle

  19. Waste Separations and Pretreatment Workshop report

    SciTech Connect (OSTI)

    Cruse, J.M.; Harrington, R.A.; Quadrel, M.J.

    1994-01-01

    This document provides the minutes from the Waste Separations and Pretreatment Workshop sponsored by the Underground Storage Tank-Integrated Demonstration in Salt Lake City, Utah, February 3--5, 1993. The Efficient Separations and Processing-Integrated Program and the Hanford Site Tank Waste Remediation System were joint participants. This document provides the detailed minutes, including responses to questions asked, an attendance list, reproductions of the workshop presentations, and a revised chart showing technology development activities.

  20. Maximizing the Sustained Throughput of Distributed Continuous Queries

    E-Print Network [OSTI]

    Palpanas, Themis

    the query plans that maximize throughput and adhere to the input profile. Examples of data stream managementMaximizing the Sustained Throughput of Distributed Continuous Queries Ioana Stanoi, George Mihaila of execution time for every incoming data tuple, which leads to an upper bound on the rate at which tuples can

  1. Method and apparatus for maximizing throughput of indirectly heated rotary kilns

    DOE Patents [OSTI]

    Coates, Ralph L; Smoot, L. Douglas; Hatfield, Kent E

    2012-10-30

    An apparatus and method for achieving improved throughput capacity of indirectly heated rotary kilns used to produce pyrolysis products such as shale oils or coal oils that are susceptible to decomposition by high kiln wall temperatures is disclosed. High throughput is achieved by firing the kiln such that optimum wall temperatures are maintained beginning at the point where the materials enter the heating section of the kiln and extending to the point where the materials leave the heated section. Multiple high velocity burners are arranged such that combustion products directly impact on the area of the kiln wall covered internally by the solid material being heated. Firing rates for the burners are controlled to maintain optimum wall temperatures.

  2. Development of a Chemoenzymatic-like and Photoswitchable Method for the High-Throughput creation of Protein Microarrays. Application to the Analysis of the Protein/Protein Interactions Involved in the YOP Virulon from Yersinia pestis.

    SciTech Connect (OSTI)

    Camarero, J A

    2006-12-07

    Protein arrays are ideal tools for the rapid analysis of whole proteomes as well as for the development of reliable and cheap biosensors. The objective of this proposal is to develop a new ligand assisted ligation method based in the naturally occurring protein trans-splicing process. This method has been used for the generation of spatially addressable arrays of multiple protein components by standard micro-lithographic techniques. Key to our approach is the use of the protein trans-splicing process. This naturally occurring process allows the development of a truly generic and highly efficient method for the covalent attachment of proteins through its C-terminus to any solid support. This technology has been used for the creation of protein chips containing several virulence factors from the human pathogen Y. pestis.

  3. Underground storage tank integrated demonstration: Evaluation of pretreatment options for Hanford tank wastes

    SciTech Connect (OSTI)

    Lumetta, G.J.; Wagner, M.J.; Colton, N.G.; Jones, E.O.

    1993-06-01

    Separation science plays a central role inn the pretreatment and disposal of nuclear wastes. The potential benefits of applying chemical separations in the pretreatment of the radioactive wastes stored at the various US Department of Energy sites cover both economic and environmental incentives. This is especially true at the Hanford Site, where the huge volume (>60 Mgal) of radioactive wastes stored in underground tanks could be partitioned into a very small volume of high-level waste (HLW) and a relatively large volume of low-level waste (LLW). The cost associated with vitrifying and disposing of just the HLW fraction in a geologic repository would be much less than those associated with vitrifying and disposing of all the wastes directly. Futhermore, the quality of the LLW form (e.g., grout) would be improved due to the lower inventory of radionuclides present in the LLW stream. In this report, we present the results of an evaluation of the pretreatment options for sludge taken from two different single-shell tanks at the Hanford Site-Tanks 241-B-110 and 241-U-110 (referred to as B-110 and U-110, respectively). The pretreatment options examined for these wastes included (1) leaching of transuranic (TRU) elements from the sludge, and (2) dissolution of the sludge followed by extraction of TRUs and {sup 90}Sr. In addition, the TRU leaching approach was examined for a third tank waste type, neutralized cladding removal waste.

  4. Effects of physical and chemical pretreatments on the crystallinity of bagasse 

    E-Print Network [OSTI]

    Jones, Maxine Janette

    2009-05-15

    to improving product yields. The process uses lime pretreatment, which can be enhanced using new decrystallization pretreatment methods, namely hydrodynamic cavitation and shock tube pretreatment.Previous studies on biomass decrystallization showed an increase...

  5. Understanding Substrate Features Influenced by Pretreatments that Limit Biomass Deconstruction by Enzymes

    E-Print Network [OSTI]

    Gao, Xiadi

    2013-01-01

    pretreatment. Energy & Environmental Science 2011, 4:973-pretreatment. Energy & Environmental Science 2011, 4:973-production. Energy & Environmental Science 2011, Pattathil

  6. A Pre-Treatment Model for Ethanol Production Using a Colorimetric...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Pre-Treatment Model for Ethanol Production Using a Colorimetric Analysis of Starch Solutions (1 Activity) A Pre-Treatment Model for Ethanol Production Using a Colorimetric...

  7. Ionic liquid pretreatment of poplar wood at room temperature: swelling and incorporation of nanoparticles

    SciTech Connect (OSTI)

    Lucas, Marcel [Los Alamos National Laboratory; Macdonald, Brian A [Los Alamos National Laboratory; Wagner, Gregory L [Los Alamos National Laboratory; Joyce, Steven A [Los Alamos National Laboratory; Rector, Kirk D [Los Alamos National Laboratory

    2010-01-01

    Lignocellulosic biomass represents a potentially sustainable source of liquid fuels and commodity chemicals. It could satisfy the energy needs for transportation and electricity generation, while contributing substantially to carbon sequestration and limiting the accumulation of greenhouse gases in the atmosphere. Potential feedstocks are abundant and include crops, agricultural wastes, forest products, grasses, and algae. Among those feedstocks, wood is mainly constituted of three components: cellulose, hemicellulose, and lignin. The conversion process of lignocellulosic biomass typically consists of three steps: (1) pretreatment; (2) hydrolysis of cellulose and hemicellulose into fermentable sugars; and (3) fermentation of the sugars into liquid fuels (ethanol) and other commodity chemicals. The pretreatment step is necessary due to the complex structure of the plant cell wall and the chemical resistance of lignin. Most current pretreatments are energy-intensive and/or polluting. So it is imperative to develop new pretreatments that are economically viable and environmentally friendly. Recently, ionic liquids have attracted considerable interest, due to their ability to dissolve biopolymers, such as cellulose, lignin, native switchgrass, and others. Ionic liquids are also considered green solvents, since they have been successfully recycled at high yields for further use with limited efficiency loss. Also, a few microbial cellulases remain active at high ionic liquid concentration. However, all studies on the dissolution of wood in ionic liquids have been conducted so far at high temperatures, typically above 90 C. Development of alternative pretreatments at room temperature is desirable to eliminate the additional energy cost. In this study, thin sections of poplar wood were swollen at room temperature by a 3 h ionic liquid (1-ethyl-3-methylimidazolium acetate or EMIMAc) pretreatment. The pretreated sample was then exposed to an aqueous suspension of nanoparticles that resulted in the sample contraction and the deposition of nanoparticles onto the surface and embedded into the cell wall. To date, both silver and gold particles ranging in size from 40-100 nm have been incorporated into wood. Penetration of gold nanoparticles of 100 nm diameter in the cell walls was best confirmed by near-infrared confocal Raman microscopy, since the deposition of gold nanoparticles induces a significant enhancement of the Raman signal from the wood in their close proximity, an enhancement attributed to the surface-enhanced Raman effect (SERS). After rinsing with water, scanning electron microscopy (SEM) and Raman images of the same areas show that most nanoparticles remained on the pretreated sample. Raman images at different depths reveal that a significant number of nanoparticles were incorporated into the wood sample, at depths up to 4 {micro}m, or 40 times the diameter of the nanoparticles. Control experiments on an untreated wood sample resulted in the deposition of nanoparticles only at the surface and most nanoparticles were removed upon rinsing. This particle incorporation process enables the development of new pretreatments, since the nanoparticles have a high surface-to-volume ratio and could be chemically functionalized. Other potential applications for the incorporated nanoparticles include isotope tracing, catalysis, imaging agents, drug-delivery systems, energy-storage devices, and chemical sensors.

  8. Algorithms for Next-Generation High-Throughput Sequencing Technologies

    E-Print Network [OSTI]

    Kao, Wei-Chun

    2011-01-01

    in the Human Genome Project(HGP) 1 . However, because Sangerprohibitive. In the case of HGP, it took more than 13 years

  9. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    x rays from one of the superconducting bend magnets at the ALS to liquid-handling robotics has enabled the collection of 96 samples in 4 hours. Importantly, the sample format...

  10. Statistical Algorithms for High-throughput Biological Data /

    E-Print Network [OSTI]

    Jeong, Kyowon

    2013-01-01

    Ph. D. in Electrical Engineering (Communication Theory andin Electrical Engineering (Communication Theory and Systems)in Electrical Engineering (Communication Theory and Systems)

  11. Constant pressure high throughput membrane permeation testing system

    DOE Patents [OSTI]

    Albenze, Erik J.; Hopkinson, David P.; Luebke, David R.

    2014-09-02

    The disclosure relates to a membrane testing system for individual evaluation of a plurality of planar membranes subjected to a feed gas on one side and a sweep gas on a second side. The membrane testing system provides a pressurized flow of a feed and sweep gas to each membrane testing cell in a plurality of membrane testing cells while a stream of retentate gas from each membrane testing cell is ported by a retentate multiport valve for sampling or venting, and a stream of permeate gas from each membrane testing cell is ported by a permeate multiport valve for sampling or venting. Back pressure regulators and mass flow controllers act to maintain substantially equivalent gas pressures and flow rates on each side of the planar membrane throughout a sampling cycle. A digital controller may be utilized to position the retentate and permeate multiport valves cyclically, allowing for gas sampling of different membrane cells over an extended period of time.

  12. Microchip Technology for High-Throughput Cancer Pathology

    E-Print Network [OSTI]

    Duberow, Jr, David Paul

    2009-01-01

    Stranded DNA Analysis. Analytical Chemistry, 2007. 79(22):Electrophoresis Chips. Analytical Chemistry, 1995. 67(20):Acid Analysis. Analytical Chemistry, 1999. 71(23): p. 5354-

  13. NETL Studies High Throughput Membrane Screening | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and economical method for gas separations from power plant flue gas streams. Polymer membranes and supported liquid membranes show great promise to solve problems in the...

  14. Integrated microbioreactor arrays for high-throughput experimentation

    E-Print Network [OSTI]

    Lee, Harry, 1976-

    2006-01-01

    Bioprocesses with microbial cells play an important role in producing biopharmaceuticals such as human insulin and human growth hormone, and other products such as amino acids and biopolymers. Because bioprocesses involve ...

  15. High-throughput pipetting with the programmable Pipetman Concept

    E-Print Network [OSTI]

    Cai, Long

    Concept® takes advantage of a PC-to-pipette connection to outperform traditional motorized pipettes include aspiration, dispensing, dilution, mixing, repetitive dispensing and many more. A few minutes

  16. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    and large-area graphene plane as a floor. Preparation ofgraphene was dried and prepared for dye coating. Dye Preparation and

  17. High mass throughput particle generation using multiple nozzle spraying

    DOE Patents [OSTI]

    Pui, David Y. H. (Plymouth, MN); Chen, Da-Ren (Creve Coeur, MO)

    2009-03-03

    Spraying apparatus and methods that employ multiple nozzle structures for producing multiple sprays of particles, e.g., nanoparticles, for various applications, e.g., pharmaceuticals, are provided. For example, an electrospray dispensing device may include a plurality of nozzle structures, wherein each nozzle structure is separated from adjacent nozzle structures by an internozzle distance. Sprays of particles are established from the nozzle structures by creating a nonuniform electrical field between the nozzle structures and an electrode electrically isolated therefrom.

  18. Differential analysis of high-throughput quantitative genetic interaction data

    E-Print Network [OSTI]

    Bean, Gordon J; Ideker, Trey

    2012-01-01

    DNA damage response (DDR)). Other biological networks, suchespecially in context of the DDR. Thus, differentialThese gene pairs indicate DDR- relevant interactions that

  19. High throughput analysis of samples in flowing liquid

    DOE Patents [OSTI]

    Ambrose, W. Patrick (Los Alamos, NM); Grace, W. Kevin (Los Alamos, NM); Goodwin, Peter M. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Orden, Alan Van (Fort Collins, CO); Keller, Richard A. (White Rock, NM)

    2001-01-01

    Apparatus and method enable imaging multiple fluorescent sample particles in a single flow channel. A flow channel defines a flow direction for samples in a flow stream and has a viewing plane perpendicular to the flow direction. A laser beam is formed as a ribbon having a width effective to cover the viewing plane. Imaging optics are arranged to view the viewing plane to form an image of the fluorescent sample particles in the flow stream, and a camera records the image formed by the imaging optics.

  20. BLINK: A High Throughput Link Layer for Backscatter Communication

    E-Print Network [OSTI]

    Ganesan, Deepak

    in areas where small form-factor is critical (e.g. on-body sen- sors for gesture and activity recognition to active radios in urban sensing deployments -- com- munication is powered by a reader, thereby making sev- eral optimizations including 1) understanding of multi-path self-interference characteristics

  1. High-Throughput Transfer Imprinting for Organic Semiconductors 

    E-Print Network [OSTI]

    Choo, Gihoon

    2013-08-06

    .......................................................... 9 Figure 5 Major steps of UV nanoimprint process ................................................... 11 Figure 6 Two methods of roller nanoimprint lithography ....................................... 13 Figure 7 Steps of LADI process... of the photolithography process are illustrated in Figure1. The first step is coating UV sensitive photoresist on the substrate. A spin-coating method is widely used for the step. After coating, a short bake at about 90C is applied to remove solution from the resist...

  2. Statistical Algorithms for High-throughput Biological Data /

    E-Print Network [OSTI]

    Jeong, Kyowon

    2013-01-01

    peptide-level factual FDR and PSM-level FDR 100 Table 4.11:affects the results . . . 4.4.8 PSM-level vs Peptide-level2 A peptide-spectrum match (PSM) is a pair (P, S) formed by

  3. Microchip Technology for High-Throughput Cancer Pathology

    E-Print Network [OSTI]

    Duberow, Jr, David Paul

    2009-01-01

    S. , and Marra, M. Resuspension of DNA Sequencing Reactionstability. (A) Sample resuspension in low-concentrationand used as the sole resuspension buffer following sample

  4. High-throughput comparison of gene fitness among related bacteria

    E-Print Network [OSTI]

    2012-01-01

    5096–5108. 36. Macnab RM: How bacteria assemble. Annu Revgene fitness among related bacteria. BMC Genomics 2012 13:gene fitness among related bacteria Rocio Canals 1 , Xiao-

  5. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    adjusted as an in-line graphene measurement technique.fabrication and metrology of graphene materials. Our mainof Large-Area Hybrid Graphene-CNT Architectures 2.1

  6. MATHEMATICAL CHALLENGES IN HIGH-THROUGHPUT MICROCALORIMETER SPECTROSCOPY

    E-Print Network [OSTI]

    -Xavier Proulx, Universit´e de Montr´eal Yun Wei, University of Michigan Heng Zhu, University of Calgary Jielin sensor systems (Figure 1) have been developed at NIST, NASA, and else- where to measure the energy. These microcalorimeters have demonstrated relative energy resolution, depend- ing on the energy band, of better than 3

  7. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientists have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The Structurally...

  8. High-throughput liquid-absorption preconcentrator sampling methods

    DOE Patents [OSTI]

    Zaromb, S.

    1994-07-12

    A system for detecting trace concentrations of an analyte in air includes a preconcentrator for the analyte and an analyte detector. The preconcentrator includes an elongated tubular container comprising a wettable material. The wettable material is continuously wetted with an analyte-sorbing liquid which flows from one part of the container to a lower end. Sampled air flows through the container in contact with the wetted material with a swirling motion which results in efficient transfer of analyte vapors or aerosol particles to the sorbing liquid and preconcentration of traces of analyte in the liquid. The preconcentrated traces of analyte may be either detected within the container or removed therefrom for injection into a separate detection means or for subsequent analysis. 12 figs.

  9. Algorithms for Next-Generation High-Throughput Sequencing Technologies

    E-Print Network [OSTI]

    Kao, Wei-Chun

    2011-01-01

    Algorithm . . . . . . . . . . . . . . . . . . . . . .2.6.1 A hybrid base-calling algorithm . . . . . . . . .Algorithm . . . . . . . . . . . . . . . . . . . . . . . . . .

  10. Towards Experimental Annotation of Genes by High Throughput Sequencing

    SciTech Connect (OSTI)

    Bradbury, Andrew

    2010-06-03

    Andrew Bradbury of Los Alamos National Laboratory discusses turning annotation into a sequencing pipeline on June 3, 2010 at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM

  11. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    exfoliated graphene oxide layers along with CVD grown CNTsto create a barrier oxide layer on copper foil. Fe catalystcopper oxide foil, the oxide layer would be gradually flow,

  12. High mass throughput particle generation using multiple nozzle spraying

    DOE Patents [OSTI]

    Pui, David Y. H.; Chen, Da-Ren

    2015-06-09

    Spraying apparatus and methods that employ multiple nozzle structures for producing multiple sprays of particles, e.g., nanoparticles, for various applications, e.g., pharmaceuticals, are provided. For example, an electrospray dispensing device may include a plurality of nozzle structures, wherein each nozzle structure is separated from adjacent nozzle structures by an internozzle distance. Sprays of particles are established from the nozzle structures by creating a nonuniform electrical field between the nozzle structures and an electrode electrically isolated therefrom.

  13. High-throughput methods for characterizing the immune repertoire

    E-Print Network [OSTI]

    Laserson, Uri

    2013-01-01

    The adaptive immune system is one of the primary mediators in almost every major human disease, including infections, cancer, autoimmunity, and inflammation-based disorders. It fundamentally functions as a molecular ...

  14. Computational tools for high-throughput discovery in biology

    E-Print Network [OSTI]

    Jones, Neil Christopher

    2007-01-01

    implementing “better” Grid security within a VO occurs whenPearlman, and S. Tuecke. Security for grid services. In 12thGrid), which is a sort of metaservice collection that handles indexing, discovery, security,

  15. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    graphene industrially, in areas such as energy, semiconductorgraphene to n- and p-type, which are required for complementary metal oxide semiconductor (

  16. Microfluidic Technologies for High-Throughput Screening Applications

    E-Print Network [OSTI]

    Quake, Stephen R.

    -based dynamic formation of picoliter-sized water droplets in a continuously flowing oil-surfactant stream for all of his valuable combinatorial chemistry advice and the generous use of lab space and supplies and electronics. Second, I would like to thank a large number of professors outside of my field that directly

  17. Computational tools for high-throughput discovery in biology

    E-Print Network [OSTI]

    Jones, Neil Christopher

    2007-01-01

    Towards creating a compute power grid. Cluster Computing andfor all data processing: the power grid. With a well-frameddevelopers. Since this Power Grid analogy has more or less

  18. METHODS AND APPLICATIONS High-throughput instant quantification of

    E-Print Network [OSTI]

    Ihee, Hyotcherl

    based on photoactive yellow protein turn off/on label Youngmin Kim,1,2 Prabhakar Ganesan,1 on the Photoactive yellow protein turn Off/On Label (POOL) system that can instantly quan- tify the concentration Gel Electrophoresis; POOL, PYP turn Off/On Label; PYP, Photoactive Yellow Protein; SUMO, Small

  19. High Throughput Analysis of Breast Cancer Specimens on the Grid

    E-Print Network [OSTI]

    cancer accounts for about 30% of all cancers and 15% of all cancer deaths in women in the United States in the United States. Women living in North America have the highest rate of breast cancer in the world [1]. In spite of the increase in the incidence of the disease, the death rates of breast cancer continue

  20. An apparatus for high throughput muscle cell experimentation

    E-Print Network [OSTI]

    Garcia-Webb, Michael G. (Michael Gregory)

    2006-01-01

    The cardiac ventricular muscle cell (myocyte) is a key experimental system for exploring the mechanical properties of the diseased and healthy heart. The myocyte experimental model provides a higher level of physiological ...

  1. Advanced method for high-throughput expression of mutated eukaryotic...

    Office of Scientific and Technical Information (OSTI)

    ; Murata, Takeshi 2 ; Department of Medical Chemistry, Kyoto University, Faculty of Medicine, Konoe-cho, Yoshida, Sakyo-Ku, Kyoto 606-8501 4 ; Abe, Keiko 1 ; Iwata, So 2 ;...

  2. High-Throughput Single-Cell Manipulation in Brain Tissue

    E-Print Network [OSTI]

    Steinmeyer, Joseph Daly

    The complexity of neurons and neuronal circuits in brain tissue requires the genetic manipulation, labeling, and tracking of single cells. However, current methods for manipulating cells in brain tissue are limited to ...

  3. Microchip Technology for High-Throughput Cancer Pathology

    E-Print Network [OSTI]

    Duberow, Jr, David Paul

    2009-01-01

    30 V/cm), followed by direct injection of analyte from thethen tested, although direct injection of 4 µL (12 x 10 6is observed following direct injection from waste to anode (

  4. High-throughput Synthesis and Metrology of Graphene Materials

    E-Print Network [OSTI]

    Ghazinejad, Maziar Ghazinejad

    2012-01-01

    the automation of the technique for production-linethe automation of the technique for production-line

  5. High-throughput Microfluidic Screening Platforms for Microalgae Study 

    E-Print Network [OSTI]

    Kim, Hyun Soo

    2014-12-15

    Microalgae have been envisioned as a future source of renewable energy. Both fossil fuel depletion and environmental concern have drawn more interest in microalgal biofuels, but the production cost of these biofuels are not yet economically...

  6. Potential of High-Throughput Experimentation with Ammonia Borane Solid

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankADVANCED MANUFACTURINGEnergy Bills andOrder 422.1,anEnergyDepartment ofPotential Health-Hydrogen

  7. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration

  8. High-Throughput Characterization of Porous Materials Using Graphics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid you notHeat Pumps Heat Pumps An error occurred.

  9. Constant pressure high throughput membrane permeation testing system

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing BacteriaConnect Collider Tests ofO y''Softening-Bipolaron''Article) |(Patent) |

  10. Constant pressure high throughput membrane permeation testing system

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing BacteriaConnect Collider Tests ofO y''Softening-Bipolaron''Article) |(Patent)

  11. Advanced method for high-throughput expression of mutated eukaryotic

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnicalInformation4563Abuse Tolerance(Conference) |stabilizedmembrane proteins in

  12. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation of Fe(II) byMultidayAlumni > The Energy Materials CenterRobotics Key to

  13. Robust, High-Throughput Analysis of Protein Structures

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation of Fe(II) byMultidayAlumni > The Energy Materials CenterRobotics Key toRobust,

  14. Throughput-cost analysis of optical flow switching

    E-Print Network [OSTI]

    Chan, Vincent W. S.

    In this paper, we employ a cost model embodying major sources of capital expenditure (CapEx) to compare the throughput-cost tradeoff offered by optical flow switching to that of more traditional optical network architectures.

  15. Outage-based throughput in wireless packet networks

    E-Print Network [OSTI]

    Win, Moe Z.

    With the increased competition for the electromagnetic spectrum, it is important to characterize the impact of interference in the performance of a wireless packet network, which is traditionally measured by its throughput. ...

  16. HANFORD MEDIUM & LOW CURIE WASTE PRETREATMENT PROJECT PHASE 1 LAB REPORT

    SciTech Connect (OSTI)

    HAMILTON, D.W.

    2006-01-30

    A fractional crystallization (FC) process is being developed to supplement tank waste pretreatment capabilities provided by the Waste Treatment and Immobilization Plant (WTP). FC can process many tank wastes, separating wastes into a low-activity fraction (LAW) and high-activity fraction (HLW). The low-activity fraction can be immobilized in a glass waste form by processing in the bulk vitrification (BV) system.

  17. Modelling the Impact of User Mobility on the Throughput in Networks of Wireless 802.11 LANs

    E-Print Network [OSTI]

    van der Mei, Rob

    Modelling the Impact of User Mobility on the Throughput in Networks of Wireless 802.11 LANs Sandjai@feweb.vu.nl Abstract-- The wireless LAN technology 802.11, also called Wi-Fi, offers high speed wireless Internet by simulation results. I. INTRODUCTION The wireless LAN (WLAN) industry is one of the fastest growing segments

  18. Suppression of alkylating agent induced cell transformation and gastric ulceration by low-dose alkylating agent pretreatment

    SciTech Connect (OSTI)

    Onodera, Akira; Department of Pharmaceutical Sciences, Kobegakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe 650-8586 ; Kawai, Yuichi; Kashimura, Asako; Ogita, Fumiya; Tsutsumi, Yasuo; Itoh, Norio

    2013-06-14

    Highlights: •Low-dose MNNG pretreatment suppresses high-dose MNNG induced in vitro transformation. •Gastric ulcers induced by high-dose MNNG decreased after low-dose MNNG pretreatment. •Efficacy of low-dose MNNG related to resistance of mutation and oxidative stress. -- Abstract: Exposure to mild stress by chemicals and radiation causes DNA damage and leads to acquired stress resistance. Although the linear no-threshold (LNT) model of safety assessment assumes risk from any dose, evidence from radiological research demonstrates a conflicting hormetic phenomenon known as the hormesis effect. However, the mechanisms underlying radiation hormesis have not yet been clarified, and little is known about the effects of low doses of chemical carcinogens. We analyzed the efficacy of pretreatment with low doses of the alkylating agent N-methyl-N?-nitro-N-nitrosoguanidine (MNNG) on the subsequent induction of cell transformation and gastric ulceration by high-dose MNNG. We used an in vitro Balb/3T3 A31-1-1 cell transformation test and monitored the formation of gastric ulcers in 5-week-old male ICR mice that were administered MNNG in drinking water. The treatment concentrations of MNNG were determined by the cell survival rate and past reports. For low-dose in vitro and in vivo experiments, MNNG was used at 0.028 ?M, and 2.8 ?g/mL, respectively. The frequency of cell transformation induced by 10 ?m MNNG was decreased by low-dose MNNG pretreatment to levels similar to that of spontaneous transformation. In addition, reactive oxygen species (ROS) and mutation frequencies induced by 10 ?m MNNG were decreased by low-dose MNNG pretreatment. Importantly, low-dose MNNG pretreatment had no effect on cell proliferation. In vivo studies showed that the number of gastric ulcers induced by 1 mg/mL MNNG decreased after low-dose MNNG pretreatment. These data indicate that low-dose pretreatment with carcinogens may play a beneficial role in the prevention of chemical toxicity under specified conditions.

  19. Method and apparatus for gasifying with a fluidized bed gasifier having integrated pretreating facilities

    DOE Patents [OSTI]

    Rice, Louis F. (Arcadia, CA)

    1981-01-01

    An integral gasifier including a pretreater section and a gasifier section separated by a distribution grid is defined by a single vessel. The pretreater section pretreats coal or other carbon-containing material to be gasified to prevent caking and agglomeration of the coal in the gasifier. The level of the coal bed of the pretreater section and thus the holding or residence time in said bed is selectively regulated by the amount of pretreated coal which is lifted up a lift pipe into the gasifier section. Thus, the holding time in the pretreater section can be varied according to the amount of pretreat necessary for the particular coal to be gasified.

  20. Characterization and Pre-treatment of LLW in Turkey - 12572

    SciTech Connect (OSTI)

    Osmanlioglu, Ahmet Erdal

    2012-07-01

    Pre-treatment of radioactive waste is the first step in waste management program that occurs after waste generation from various applications in Turkey. Pre-treatment and characterization practices are carried out in Radioactive Waste Management Unit (RWMU) at Cekmece Nuclear Research and Training Center (CNRTC) in Istanbul. This facility has been assigned to take all low-level radioactive wastes generated by nuclear applications in Turkey. The wastes are generated from research and nuclear applications mainly in medicine, biology, agriculture, quality control in metal processing and construction industries. These wastes are classified as low- level radioactive wastes. Pre-treatment practices cover several steps. In this paper, main steps of pre-treatment and characterization are presented. Basically these are; collection, segregation, chemical adjustment, size reduction and decontamination operations. (author)

  1. Ammonia fiber explosion (AFEX) pretreatment of municipal solid waste components 

    E-Print Network [OSTI]

    Lundeen, Joseph Eric

    1991-01-01

    AMMONIA FIBER EXPLOSION (AFEX) PRETREATMENT OF MUNICIPAL SOLID WASTE COMPONENTS A Thesis by JOSEPH ERIC LUNDEEN Submitted to the Oflice of Graduate Studies of Texas A&M University in partial fulfdlment of the requirements for the degree... of MASTER OF SCIENCE December 1991 Major Subject: Chemical Engineering AMMONIA FIBER EXPLOSION (AFEX) PRETREATMENT OF MUNICIPAL SOLID WASTE COMPONENTS A Thesis Joseph Eric Lundeen Approved as to style and content by: Mark Hol pie ( tte R. W...

  2. Method to Produce Highly Digestible, Pretreated Lignocellulosic Biomass

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on dark matterEnergy Innovation PortalPrinceton PlasmaUsing

  3. Radioactive waste tank Initial Pretreatment Module (IPM) technology development and selection

    SciTech Connect (OSTI)

    Beeman, G.H. [Pacific Northwest Lab., Richland, WA (United States); Hansrote, G. [Westinghouse Hanford Co., Richland, WA (United States)

    1994-03-01

    The processing of nuclear materials at the Hanford Site has resulted in the accumulation of radioactive wastes stored in 177 single- and double-shell tanks (SSTs and DSTs). Fifty-four of the 177 tanks are currently on a tank watch list because organic chemicals and ferrocyanide compounds in the tanks present a potential fire or explosion hazard. In addition, one additional SST is under consideration for placement on the watch list because of high organic concentration. Seventeen of the watch list tanks require pretreatment, and two DST complexant concentrate waste tanks not on the watch list may also need pretreatment. The proposed Initial Pretreatment Module (IPM) is expected to resolve the safety concerns by destroying the organics and ferrocyanide compounds in the tank wastes. The primary objective of the IPM is to destroy or modify constituents that cause safety concerns in the watch list tanks. A secondary objective is to enhance the cost effectiveness of processing the wastes by performing additional processing. Overall, IPM will achieve organic/ferrocyanide destruction (the primary goal) and will assist in the separation of cesium, strontium, and technetium from the tank wastes.

  4. Pretreatment status report on the identification and evaluation of alternative processes. Milestone Report No. C064

    SciTech Connect (OSTI)

    Sutherland, D.G.; Brothers, A.J.; Beary, M.M.; Nicholson, G.A.

    1993-09-01

    The purpose of this report is to support the development and demonstration of a pretreatment system that will (1) destroy organic materials and ferrocyanide in tank wastes so that the wastes can be stored safely, (2) separate the high-activity and low-activity fractions, (3) remove radionuclides and remove or destroy hazardous chemicals in LLW as necessary to meet waste form feed requirements, (4) support development and demonstration of vitrification technology by providing representative feeds to the bench-scale glass melter, (5) support full-scale HLW vitrification operations, including near-term operation, by providing feed that meets specifications, and (6) design and develop pretreatment processes that accomplish the above objectives and ensure compliance with environmental regulations. This report is a presentation of candidate technologies for pretreatment of Hanford Site tank waste. Included are descriptions of studies by the Pacific Northwest Laboratory of Battelle Memorial Institute; Science Applications International Corporation, an independent consultant; BNFL, Inc. representing British technologies; Numatec, representing French technologies; and brief accounts of other relevant activities.

  5. Interim data quality objectives for waste pretreatment and vitrification. Revision 1

    SciTech Connect (OSTI)

    Kupfer, M.J.; Conner, J.M.; Kirkbride, R.A.; Mobley, J.R.

    1994-09-15

    The Tank Waste Remediation System (TWRS) is responsible for storing, processing, and immobilizing the Hanford Site tank wastes. Characterization information on the tank wastes is needed so that safety concerns can be addressed, and retrieval, pretreatment, and immobilization processes can be designed, permitted, and implemented. This document describes the near-term tank waste sampling and characterization needs of the Pretreatment, High-Level Waste (HLW) Disposal, and Low-Level Waste (LLW) Disposal Programs to support the TWRS disposal mission. The final DQO (Data Quality Objective) will define specific waste tanks to be sampled, sample timing requirements, an appropriate analytical scheme, and a list of required analytes. This interim DQO, however, focuses primarily on the required analytes since the tanks to be sampled in FY 1994 and early FY 1995 are being driven most heavily by other considerations, particularly safety. The major objective of this Interim DQO is to provide guidance for tank waste characterization requirements for samples taken before completion of the final DQO. The characterization data needs defined herein will support the final DQO to help perform the following: Support the TWRS technical strategy by identification of the chemical and physical composition of the waste in the tanks and Guide development efforts to define waste pretreatment processes, which will in turn define HLW and LLW feed to vitrification processes.

  6. Tank Waste Remediation System tank waste pretreatment and vitrification process development testing requirements assessment

    SciTech Connect (OSTI)

    Howden, G.F.

    1994-10-24

    A multi-faceted study was initiated in November 1993 to provide assurance that needed testing capabilities, facilities, and support infrastructure (sampling systems, casks, transportation systems, permits, etc.) would be available when needed for process and equipment development to support pretreatment and vitrification facility design and construction schedules. This first major report provides a snapshot of the known testing needs for pretreatment, low-level waste (LLW) and high-level waste (HLW) vitrification, and documents the results of a series of preliminary studies and workshops to define the issues needing resolution by cold or hot testing. Identified in this report are more than 140 Hanford Site tank waste pretreatment and LLW/HLW vitrification technology issues that can only be resolved by testing. The report also broadly characterizes the level of testing needed to resolve each issue. A second report will provide a strategy(ies) for ensuring timely test capability. Later reports will assess the capabilities of existing facilities to support needed testing and will recommend siting of the tests together with needed facility and infrastructure upgrades or additions.

  7. Optimizing Throughput and Energy in Online Deadline Scheduling

    E-Print Network [OSTI]

    Wong, Prudence W.H.

    consider a processor that can vary its speed between 0 and a maximum speed T to minimize its energy usage usage. If we ignore energy usage, the best possible online algorithm is 4-competitive on throughput jobs; O(1)-competitive online algorithms with respect to energy usage have been known [Yao et al. 1995

  8. On the Throughput Capacity of Heterogeneous Wireless Networks

    E-Print Network [OSTI]

    Latchman, Haniph A.

    On the Throughput Capacity of Heterogeneous Wireless Networks Pan Li, Member, IEEE, and Yuguang of wireless networks. However, it is commonly assumed that all nodes in the network are identical. The issue of heterogeneous wireless networks with general network settings. Specifically, we consider an extended network

  9. Maximizing Throughput in Wireless Networks with Finite Internal Buffers

    E-Print Network [OSTI]

    Chang, Cheng-Shang

    Maximizing Throughput in Wireless Networks with Finite Internal Buffers Ching-Min Lien, Cheng of a discrete-time wireless network, where only certain sets of links can transmit simultaneously. It is well of the configuration vectors determines the capacity region of the wireless network. In the literature, packet

  10. Understanding TCP Incast Throughput Collapse in Datacenter Networks

    E-Print Network [OSTI]

    Joseph, Anthony D.

    Understanding TCP Incast Throughput Collapse in Datacenter Networks Yanpei Chen, Rean Griffith datacenters support a myriad of services and ap- plications. Companies like Google, Microsoft, Yahoo, and Amazon use datacenters for web search, storage, e-commerce, and large-scale general computations

  11. Storage Performance Virtualization via Throughput and Latency Control

    E-Print Network [OSTI]

    Riska, Alma

    Storage Performance Virtualization via Throughput and Latency Control JIANYONG ZHANG, ANAND storage systems. A consequence of this trend is the need to serve multiple users and/or workloads of the underlying storage system; (2) couple rate and latency allocation in a single scheduler, making them less

  12. FLUID: Improving Throughputs in Enterprise Wireless LANs through Flexible Channelization

    E-Print Network [OSTI]

    Liblit, Ben

    the usefulness of flexible channels in the context of 802.11-based networks. Current 802.11 hardware can provideFLUID: Improving Throughputs in Enterprise Wireless LANs through Flexible Channelization Shravan for designing 802.11 wireless LANs (WLANs) using flexible channelization -- the choice of an appropriate channel

  13. Thread Row Buffers: Improving Memory Performance Isolation and Throughput

    E-Print Network [OSTI]

    Tullsen, Dean M.

    and energy efficiency. Furthermore, concurrent execution of applications also has shown the needThread Row Buffers: Improving Memory Performance Isolation and Throughput in Multiprogrammed of performance isolation among threads in the memory controller to enforce a quality of service in virtualized

  14. Access Point Selection for Improving Throughput Fairness in Wireless LANs

    E-Print Network [OSTI]

    Siris, Vasilios A.

    Access Point Selection for Improving Throughput Fairness in Wireless LANs Vasilios A. Siris investigate the problem of access point selection in wireless LANs based on the IEEE 802.11 standard, when solely at the wireless stations, which passively monitor the activity of each access point's channel

  15. Enhanced resolution and throughput of Fresnel incoherent correlation holography (FINCH)

    E-Print Network [OSTI]

    Rosen, Joseph

    Enhanced resolution and throughput of Fresnel incoherent correlation holography (FINCH) using dual Montgomery County Campus, Rockville, Maryland 20850, USA * barakk@ee.bgu.ac.il Abstract: Fresnel incoherent Fresnel holography," Opt. Lett. 32(8), 912­914 (2007). 7. J. Rosen and G. Brooker, "Fluorescence

  16. Reliability and Throughput in Future Automotive Communication Networks

    E-Print Network [OSTI]

    Rajkumar, Ragunathan "Raj"

    Reliability and Throughput in Future Automotive Communication Networks John Liu, Timothy TaltyStar. This action created a new industry called automotive telematics, helping to save lives and providing, the automotive telematics industry has experienced a healthy growth in North America. During the current economic

  17. Dynamic Power Allocation For Maximizing Throughput in Energy Harvesting

    E-Print Network [OSTI]

    Vaze, Rahul

    1 Dynamic Power Allocation For Maximizing Throughput in Energy Harvesting Communication System general case of arbitrarily varying energy arrivals is considered, where neither the future energy arrival strategy that invests available energy uniformly over all remaining slots until the next energy arrival

  18. Methods of pretreating comminuted cellulosic material with carbonate-containing solutions

    DOE Patents [OSTI]

    Francis, Raymond

    2012-11-06

    Methods of pretreating comminuted cellulosic material with an acidic solution and then a carbonate-containing solution to produce a pretreated cellulosic material are provided. The pretreated material may then be further treated in a pulping process, for example, a soda-anthraquinone pulping process, to produce a cellulose pulp. The pretreatment solutions may be extracted from the pretreated cellulose material and selectively re-used, for example, with acid or alkali addition, for the pretreatment solutions. The resulting cellulose pulp is characterized by having reduced lignin content and increased yield compared to prior art treatment processes.

  19. High-Throughput, High-Precision Hot Testing Tool for High-Brightness...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Diode Testing Lead Performer: KLA-Tencor Corporation - Milpitas, CA Partners: Ocean Optics - Dunedin, FL DOE Total Funding: 3,994,729 Cost Share: 4,626,422 Project Term: 815...

  20. Wireless Throughput and Energy Efficiency with Random Arrivals and Statistical Queueing Constraints

    E-Print Network [OSTI]

    Gursoy, Mustafa Cenk

    Wireless Throughput and Energy Efficiency with Random Arrivals and Statistical Queueing Constraints Mustafa Ozmen and M. Cenk Gursoy Abstract--Throughput and energy efficiency in fading channels are studied. Throughput is further studied in certain asymptotic regimes. Furthermore, energy efficiency is analyzed

  1. Project Profile: High Operating Temperature Liquid Metal Heat...

    Office of Environmental Management (EM)

    multi-target co-sputtering system to create massive compositional libraries in thin-film forms and employ high-throughput characterization methods to rapidly screen candidate...

  2. NREL Breaks New Ground in Plant Pretreatment for Biofuels (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2013-01-01

    NREL researchers use imaging technologies to broaden knowledge of plant cell wall structures and identify ideal pretreatment of plant material.

  3. BIOETHANOL PRODUCTION FROM WET OXIDSED CORN STOVER USING PRE-TREATED MANURE AS A NUTRIENT SOURCE

    E-Print Network [OSTI]

    and oxygen addition) were also applied for pre-treatment of manure. The manure was blended with water for the production of bioethanol. This pre-treatment method, similar to other hot water pre-treatments, acts, Roskilde, Denmark ABSTRACT: In the present study ethanol was produced from wet oxidised corn stover

  4. Coal liquefaction process using pretreatment with a binary solvent mixture

    DOE Patents [OSTI]

    Miller, Robert N. (Allentown, PA)

    1986-01-01

    An improved process for thermal solvent refining or hydroliquefaction of non-anthracitic coal at elevated temperatures under hydrogen pressure in a hydrogen donor solvent comprises pretreating the coal with a binary mixture of an aromatic hydrocarbon and an aliphatic alcohol at a temperature below 300.degree. C. before the hydroliquefaction step. This treatment generally increases both conversion of coal and yields of oil.

  5. Effects of catalyst pretreatment for carbon nanotube growth

    E-Print Network [OSTI]

    Morgan, Caitlin D

    2007-01-01

    The effects of pretreatment of iron catalyst for carbon nanotube (CNT) growth was studied. CNTs were grown on Fe/A1203 (1/10 nm) thin-film catalyst deposited on silicon substrates via exposure to C2H4 in a thermal chemical ...

  6. Separations/pretreatment considerations for Hanford privatization phase 2

    SciTech Connect (OSTI)

    Hunt, R.D.; McGinnis, C.P.; Welch, T.D.

    1998-05-01

    The Tank Focus Area is funded to develop, demonstrate, and deploy technologies that will assist in the treatment and closure of its nuclear waste tanks. Pretreatment technologies developed to support the privatization effort by the Department of Energy are reviewed. Advancements in evaporation, solid-liquid separation, sludge treatment, solids controls, sodium management, and radionuclide removal are considered.

  7. A review on biomass classification and composition, cofiring issues and pretreatment methods

    SciTech Connect (OSTI)

    Jaya Shankar Tumuluru; Shahab Sokhansanj; Christopher T. Wright; Richard D. Boardman

    2011-08-01

    Presently around the globe there is a significant interest in using biomass for power generation as power generation from coal continues to raise environmental concerns. Biomass alone can be used for generation of power which can bring lot of environmental benefits. However the constraints of using biomass alone can include high investments costs for biomass feed systems and also uncertainty in the security of the feedstock supply due to seasonal variations and in most of the countries biomass is dispersed and the infrastructure for biomass supply is not well established. Alternatively cofiring biomass along with coal offer advantages like (a) reducing the issues related to biomass quality and buffers the system when there is insufficient feedstock quantity and (b) costs of adapting the existing coal power plants will be lower than building new systems dedicated only to biomass. However with the above said advantages there exists some technical constrains including low heating and energy density values, low bulk density, lower grindability index, higher moisture and ash content to successfully cofire biomass with coal. In order to successfully cofire biomass with coal, biomass feedstock specifications need to be established to direct pretreatment options that may include increasing the energy density, bulk density, stability during storage and grindability. Impacts on particle transport systems, flame stability, pollutant formation and boiler tube fouling/corrosion must also be minimized by setting feedstock specifications including composition and blend ratios if necessary. Some of these limitations can be overcome by using pretreatment methods. This paper discusses the impact of feedstock pretreatment methods like sizing, baling, pelletizing, briquetting, washing/leaching, torrefaction, torrefaction and pelletization and steam explosion in attainment of optimum feedstock characteristics to successfully cofire biomass with coal.

  8. Pretreatment Engineering Platform Phase 1 Final Test Report

    SciTech Connect (OSTI)

    Kurath, Dean E.; Hanson, Brady D.; Minette, Michael J.; Baldwin, David L.; Rapko, Brian M.; Mahoney, Lenna A.; Schonewill, Philip P.; Daniel, Richard C.; Eslinger, Paul W.; Huckaby, James L.; Billing, Justin M.; Sundar, Parameshwaran S.; Josephson, Gary B.; Toth, James J.; Yokuda, Satoru T.; Baer, Ellen BK; Barnes, Steven M.; Golovich, Elizabeth C.; Rassat, Scot D.; Brown, Christopher F.; Geeting, John GH; Sevigny, Gary J.; Casella, Amanda J.; Bontha, Jagannadha R.; Aaberg, Rosanne L.; Aker, Pamela M.; Guzman-Leong, Consuelo E.; Kimura, Marcia L.; Sundaram, S. K.; Pires, Richard P.; Wells, Beric E.; Bredt, Ofelia P.

    2009-12-23

    Pacific Northwest National Laboratory (PNNL) was tasked by Bechtel National Inc. (BNI) on the River Protection Project, Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to conduct testing to demonstrate the performance of the WTP Pretreatment Facility (PTF) leaching and ultrafiltration processes at an engineering-scale. In addition to the demonstration, the testing was to address specific technical issues identified in Issue Response Plan for Implementation of External Flowsheet Review Team (EFRT) Recommendations - M12, Undemonstrated Leaching Processes.( ) Testing was conducted in a 1/4.5-scale mock-up of the PTF ultrafiltration system, the Pretreatment Engineering Platform (PEP). Parallel laboratory testing was conducted in various PNNL laboratories to allow direct comparison of process performance at an engineering-scale and a laboratory-scale. This report presents and discusses the results of those tests.

  9. Coal liquefaction process using pretreatment with a binary solvent mixture

    DOE Patents [OSTI]

    Miller, R.N.

    1986-10-14

    An improved process for thermal solvent refining or hydroliquefaction of non-anthracitic coal at elevated temperatures under hydrogen pressure in a hydrogen donor solvent comprises pretreating the coal with a binary mixture of an aromatic hydrocarbon and an aliphatic alcohol at a temperature below 300 C before the hydroliquefaction step. This treatment generally increases both conversion of coal and yields of oil. 1 fig.

  10. Kinetic Modeling and Assessment of Lime Pretreatment of Poplar Wood 

    E-Print Network [OSTI]

    Sierra Ramirez, Rocio

    2012-02-14

    , industrial, and agricultural). 5. Appropriate selection of feedstock and conversion technology. 6. Improved efficiency of the production technology obtained through intensive research and development. 7. More efficient use of energy, including vehicle...-1 KINETIC MODELING AND ASSESSMENT OF LIME PRETREATMENT OF POPLAR WOOD A Dissertation by ROCIO SIERRA RAMIREZ Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree...

  11. Modification of Bi:YIG film properties by substrate surface ion pre-treatment

    SciTech Connect (OSTI)

    Shaposhnikov, A.N.; Prokopov, A.R.; Karavainikov, A.V.; Berzhansky, V.N.; Mikhailova, T.V.; Kotov, V.A.; Balabanov, D.E.; Sharay, I.V.; Salyuk, O.Y.; Vasiliev, M.; Golub, V.O.

    2014-07-01

    Highlights: • Effects of substrates ion beam treatment on magnetoptical properties Bi:YIG films. • Substrate surface damage results in sign inversion of the magneto-optical effects. • Atomically smooth films growth takes place on low energy ions treated substrates. • High energy ions treatment results in selective nucleation mechanism of the growth. - Abstract: The effect of a controlled ion beam pre-treatment of (1 1 1)-oriented Gd{sub 3}Ga{sub 5}O{sub 12} substrates on the magneto-optical properties and surface morphology of the ultrathin bismuth-substituted yttrium–iron garnet films with a composition Bi{sub 2.8}Y{sub 0.2}Fe{sub 5}O{sub 12} was studied. It has been shown that the observed sign inversion of magneto-optical effects (Faraday rotation and magnetic circular dichroism) observed in films that were deposited on the GGG substrate pre-treated by 1 keV and 4 keV Ar{sup +} ion beams is a result of the substrate surface amorphization caused by the ion bombardment.

  12. Gasification performance of switchgrass pretreated with torrefaction and densification

    SciTech Connect (OSTI)

    Jaya Shankar Tumuluru; Various

    2014-08-01

    The purpose of this study was to investigate gasification performance of four switchgrass pretreatments (torrefaction at 230 and 270 °C, densification, and combined torrefaction and densification) and three gasification temperatures (700, 800 and 900 °C). Gasification was performed in a fixed-bed externally heated reactor with air as an oxidizing agent. Switchgrass pretreatment and gasification temperature had significant effects on gasification performance such as gas yields, syngas lower heating value (LHV), and carbon conversion and cold gas efficiencies. With an increase in the gasification temperature, yields of H2 and CO, syngas LHV, and gasifier efficiencies increased whereas CH4, CO2 and N2 yields decreased. Among all switchgrass pretreatments, gasification performance of switchgrass with combined torrefaction and densification was the best followed by that of densified, raw and torrefied switchgrass. Gasification of combined torrefied and densified switchgrass resulted in the highest yields of H2 (0.03 kg/kg biomass) and CO (0.72 kg/kg biomass), highest syngas LHV (5.08 MJ m-3), CCE (92.53%), and CGE (68.40%) at the gasification temperature of 900 °C.

  13. Rheological study of comingled biomass and coal slurries with hydrothermal pretreatment

    SciTech Connect (OSTI)

    Wei He; Chan S. Park; Joseph M. Norbeck [University of California, Riverside, CA (United States). Bourns College of Engineering Center for Environmental Research and Technology

    2009-09-15

    Gasification of comingled biomass and coal feedstock is an effective means of reducing the net life cycle greenhouse gas emissions in the coal gasification process while maintaining its inherent benefits of abundance and high-energy density. However, feeding a comingled biomass and coal feedstock into a pressurized gasification reactor poses a technical problem. Conventional dry feeding systems, such as lock hoppers and pressurized pneumatic transport, are complex and operationally expensive. A slurry formation of comingled biomass and coal feedstock can be easily fed into the gasification reactor but, in normal conditions, only allows for a small portion of biomass in the mixture. This is a consequence of the hydroscopic and hydrophilic nature of the biomass. The College of Engineering Center for Environmental Research and Technology (CE-CERT) at the University of California, Riverside, has developed a process producing high solid content biomass-water slurry using a hydrothermal pretreatment process. In this paper, the systematic investigation of the rheological properties (e.g., shear rate, shear stress, and viscosity) of coal-water slurries, biomass-water slurries, and comingled biomass and coal-water slurries is reported. The solid particle size distribution in the slurry and the initial solid/water ratio were investigated to determine the impact on shear rate and viscosity. This was determined using a rotational rheometer. The experimental results show that larger particle size offers better pumpability. The presence of a high percentage of biomass in solid form significantly decreases slurry pumpability. It is also shown that the solid loading of the biomass-water slurry can be increased to approximately 35 wt % with viscosity of less than 0.7 Pa.s after the pretreatment process. The solid loading increased to approximately 45 wt % when the biomass is comingled with coal. 18 refs., 7 figs., 3 tabs.

  14. How Wood Chip Size Affects Pretreatment Effectiveness of Woody Biomass for Biological Processing

    E-Print Network [OSTI]

    Tam, Jerry

    2013-01-01

    explosion pretreatment of wood: effect of chip size, acid,In: Soltes, E.J. (Ed. ), Wood and Agriculture Residues –D. , Wegener, G. , 1984. Wood: Chemistry, Ultrastructure,

  15. Bayesian Model Selection for High-dimensional High-throughput Data 

    E-Print Network [OSTI]

    Joshi, Adarsh

    2012-07-16

    , the 10 g-prior corresponds to an alternative distribution of the following form: H ?2 : ? ? N(0, c n ). (2.5) Hence the expression for the corresponding Bayes? factor can be written as BF (2|1) = ?(Y? , 0, (c + 1)/n) ?(Y? , 0, 1/n) = 1 (c + 1... to the interval (a, a) by MoM prior centered on 0 with scale ? and n = 1 is 2 [ ? ( a ? ? ) ? a ? 2pi? exp ( ? a2 2? ) ? 1 2 ] . (2.21) 17 For an iMoM prior, the corresponding probability is 1?G [ (a/ ? ?)?2k; ? 2k , 1 ] , (2...

  16. High throughput screen for cells with high extracellular metabolite consumption--secretion rates using microfluidic droplets

    E-Print Network [OSTI]

    Wang, Benjamin L. (Benjamin Lu chen)

    2009-01-01

    Metabolic engineering has contributed significantly to the improvement of strains for the industrial production of various compounds. Traditionally, enzymatic steps closely associated with the product-forming pathway have ...

  17. High throughput parallel backside contacting and periodic texturing for high-efficiency solar cells

    DOE Patents [OSTI]

    Daniel, Claus; Blue, Craig A.; Ott, Ronald D.

    2014-08-19

    Disclosed are configurations of long-range ordered features of solar cell materials, and methods for forming same. Some features include electrical access openings through a backing layer to a photovoltaic material in the solar cell. Some features include textured features disposed adjacent a surface of a solar cell material. Typically the long-range ordered features are formed by ablating the solar cell material with a laser interference pattern from at least two laser beams.

  18. Throughput-Lifetime Tradeoffs in Multihop Wireless Networks under an SINR-based

    E-Print Network [OSTI]

    Rosenberg, Catherine P.

    1 Throughput-Lifetime Tradeoffs in Multihop Wireless Networks under an SINR-based Interference crucial design objectives for a number of multihop wireless network applica- tions. As these two this tradeoff between the network throughput and lifetime, for the case of fixed wireless networks where link

  19. Maximizing Throughput of UAV-Relaying Networks with the Load-Carry-and-Deliver Paradigm

    E-Print Network [OSTI]

    Kung, H. T.

    Maximizing Throughput of UAV-Relaying Networks with the Load-Carry-and-Deliver Paradigm Chen Unmanned Aerial Vehicles (UAVs) to relay messages between two distant ground nodes. For delay-tolerant applications like latency-insensitive bulk data transfer, we seek to maximize throughput by having a UAV load

  20. Throughput Optimal Task Allocation under Thermal Constraints for Multi-core Processors

    E-Print Network [OSTI]

    Chatha, Karam S.

    Throughput Optimal Task Allocation under Thermal Constraints for Multi-core Processors Vinay and thermal hotspots affect the reliability of microprocessors. Temperature is also an important constraint. In this paper, we formulate the problem of throughput-optimal task allocation on thermally constrained multi

  1. A Switch-Based Approach to Throughput Collapse and Starvation in Data Centers Alexander Shpiner

    E-Print Network [OSTI]

    Keslassy, Isaac

    A Switch-Based Approach to Throughput Collapse and Starvation in Data Centers Alexander Shpiner on small switch buffers. However, in case of congestion, data center switches may suffer from throughput link. Last, HCF also prevents packet reordering. Key words: TCP, Data centers, Switching. 1

  2. A Switch-Based Approach to Throughput Collapse and Starvation in Data Centers

    E-Print Network [OSTI]

    Keslassy, Isaac

    A Switch-Based Approach to Throughput Collapse and Starvation in Data Centers Alexander Shpiner, 32000, Israel {shalex@tx, isaac@ee}.technion.ac.il Abstract--Data center switches need to satisfy, in case of congestion, data center switches can incur throughput collapse for short TCP flows as well

  3. Evaluation of solid-based separation materials for the pretreatment of radioactive wastes

    SciTech Connect (OSTI)

    Lumetta, G.J.; Wagner, M.J.; Wester, D.W.; Morrey, J.R.

    1993-05-01

    Separation science will play an important role in pretreating nuclear wastes stored at various US Department of Energy Sites. The application of separation processes offers potential economic and environmental benefits with regards to remediating these sites. For example, at the Hanford Site, the sizeable volume of radioactive wastes stored in underground tanks could be partitioned into a small volume of high-level waste (HLW) and a relatively large volume of low-level waste (LLW). After waste separation, only the smaller volume of HLW would require costly vitrification and geologic disposal. Furthermore, the quality of the remaining LLW form (e.g., grout) would be improved due to the lower inventory of radionuclides present in the LLW stream. This report investigates extraction chromatography as a possible separation process for Hanford wastes.

  4. Recovery of Plutonium from Refractory Residues Using a Sodium Peroxide Pretreatment Process

    SciTech Connect (OSTI)

    Rudisill, T.S.

    2003-10-23

    The recycle of plutonium from refractory residues is a necessary activity for the nuclear weapon production complex. Traditionally, high-fired plutonium oxide (PuO2) was leached from the residue matrix using a nitric acid/fluoride dissolving flowsheet. The recovery operations were time consuming and often required multiple contacts with fresh dissolving solution to reduce the plutonium concentration to levels where residual solids could be discarded. Due to these drawbacks, the development of an efficient process for the recovery of plutonium from refractory materials is desirable. To address this need, a pretreatment process was developed. The development program utilized a series of small-scale experiments to optimize processing conditions for the fusion process and demonstrate the plutonium recovery efficiency using ceramic materials developed as potential long-term storage forms for PuO2 and an incinerator ash from the Rocky Flats Environmental Technology Site (Rocky Flats) as te st materials.

  5. Preliminary design requirements document (DRD) for Project W-236B, ``Initial Pretreatment Module``

    SciTech Connect (OSTI)

    Swanson, L.M.

    1995-02-15

    The scope of this Design Requirements Document (DRD) is to identify and define the functions, with associated requirements, which must be performed to separate Hanford Site tank waste supernatants into low-level and high-level fractions. This documents sets forth function requirements, performance requirements, and design constraints necessary to begin conceptual design for the Initial Pretreatment Module (IPM). System and physical interfaces between the IPM project and the Tank Waste Remediation System (TWRS) are identified. The constraints, performance requirements, and transfer of information and data across a technical interface will be documented in an Interface Control Document. Supplemental DRDs will be prepared to provide more detailed requirements specific to systems described in the DRD.

  6. Caustic-Side Solvent-Extraction Modeling for Hanford Interim Pretreatment System

    SciTech Connect (OSTI)

    Moyer, B.A.; Birdwell, J.F.; Delmau, L. H.; McFarlane, J.

    2008-06-01

    The purpose of this work is to examine the applicability of the Caustic-Side Solvent Extraction (CSSX) process for the removal of cesium from Hanford tank-waste supernatant solutions in support of the Hanford Interim Pretreatment System (IPS). The Hanford waste types are more challenging than those at the Savannah River Site (SRS) in that they contain significantly higher levels of potassium, the chief competing ion in the extraction of cesium. It was confirmed by use of the CSSX model that the higher levels of potassium depress the cesium distribution ratio (DCs), as validated by measurement of DCs values for four of eight specified Hanford waste-simulant compositions. The model predictions were good to an apparent standard error of ±11%. It is concluded from batch distribution experiments, physical-property measurements, equilibrium modeling, flowsheet calculations, and contactor sizing that the CSSX process as currently employed for cesium removal from alkaline salt waste at the SRS is capable of treating similar Hanford tank feeds. For the most challenging waste composition, 41 stages would be required to provide a cesium decontamination factor (DF) of 5000 and a concentration factor (CF) of 5. Commercial contacting equipment with rotor diameters of 10 in. for extraction and 5 in. for stripping should have the capacity to meet throughput requirements, but testing will be required to confirm that the needed efficiency and hydraulic performance are actually obtainable. Markedly improved flowsheet performance was calculated for a new solvent formulation employing the more soluble cesium extractant BEHBCalixC6 used with alternative scrub and strip solutions, respectively 0.1 M NaOH and 10 mM boric acid. The improved system can meet minimum requirements (DF = 5000 and CF = 5) with 17 stages or more ambitious goals (DF = 40,000 and CF = 15) with 19 stages. Potential benefits of further research and development are identified that would lead to reduced costs, greater adaptability of the process to DOE alkaline salt wastes, and greater readiness for implementation. Such benefits accrue from optimal sizing of centrifugal contactors for application of the CSSX process for the IPS; more accurate modeling of cesium extraction with greater flexibility and applicability to a variety of feeds and flowsheet conditions; and further improving and optimizing the alternative CSSX solvent and scrub/strip system.

  7. Pretreated densified biomass products (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTech ConnectSpeeding accessusers'(x≤2) surface:SciTechinvestigation (JournalPretreated

  8. Waste Treatment and Immobilation Plant Pretreatment Facility | Department

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LIST OFAMERICA'S FUTURE. regulators consumerWaste Isolationof Energy Pretreatment

  9. Restricting lignin and enhancing sugar deposition in secondary cell walls enhances monomeric sugar release after low temperature ionic liquid pretreatment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Scullin, Chessa; Cruz, Alejandro G.; Chuang, Yi -De; Simmons, Blake A.; Loque, Dominique; Singh, Seema

    2015-07-04

    Lignocellulosic biomass has the potential to be a major source of renewable sugar for biofuel production. Before enzymatic hydrolysis, biomass must first undergo a pretreatment step in order to be more susceptible to saccharification and generate high yields of fermentable sugars. Lignin, a complex, interlinked, phenolic polymer, associates with secondary cell wall polysaccharides, rendering them less accessible to enzymatic hydrolysis. Herein, we describe the analysis of engineered Arabidopsis lines where lignin biosynthesis was repressed in fiber tissues but retained in the vessels, and polysaccharide deposition was enhanced in fiber cells with little to no apparent negative impact on growth phenotype.

  10. Access of Cellulase to Cellulose and Lignin for Poplar Solids Produced by Leading Pretreatment Technologies

    E-Print Network [OSTI]

    California at Riverside, University of

    Access of Cellulase to Cellulose and Lignin for Poplar Solids Produced by Leading Pretreatment. From this, Langmuir adsorption parameters, cellulose accessibility to cellulase, and the effectiveness (rsolids Ľ 56 mg/g solid). Lime pretreated sol- ids also had the highest cellulose accessibility

  11. PROJECT W-551 INTERIM PRETREATMENT SYSTEM TECHNOLOGY SELECTION SUMMARY DECISION REPORT AND RECOMMENDATION

    SciTech Connect (OSTI)

    CONRAD EA

    2008-08-12

    This report provides the conclusions of the tank farm interim pretreatment technology decision process. It documents the methodology, data, and results of the selection of cross-flow filtration and ion exchange technologies for implementation in project W-551, Interim Pretreatment System. This selection resulted from the evaluation of specific scope criteria using quantitative and qualitative analyses, group workshops, and technical expert personnel.

  12. Impact of Velocity on Corn Stover Pretreatment 977 Applied Biochemistry and Biotechnology Vol. 113116, 2004

    E-Print Network [OSTI]

    California at Riverside, University of

    with velocity, especially in the early reaction stage, suggests that chemical reaction is not the only factorImpact of Velocity on Corn Stover Pretreatment 977 Applied Biochemistry and Biotechnology Vol. 113 Velocity on Hot Water Only Pretreatment of Corn Stover in a Flowthrough Reactor CHAOGANG LIU AND CHARLES E

  13. Shaping Reactor Microbiomes to Produce the Fuel Precursor nButyrate from Pretreated Cellulosic Hydrolysates

    E-Print Network [OSTI]

    Angenent, Lars T.

    - butyrate production rate of 0.47 g COD l-1 d-1 in bioreactors that were fed with dilute-acid pretreatedShaping Reactor Microbiomes to Produce the Fuel Precursor nButyrate from Pretreated Cellulosic the production of carboxylic acids with open cultures of microbial consortia (reactor micro- biomes), we

  14. High Performance Interconnect System Design for Future Chip Multiprocessors 

    E-Print Network [OSTI]

    Wang, Lei

    2013-05-02

    constraints of power and area, and provide ultra low latencies and high throughput. In this research, we explore different techniques to design high performance NOC. First, existing NOCs mostly use Dimension Order Routing (DOR) to determine the route taken...

  15. Sci—Sat AM: Stereo — 01: 3D Pre-treatment Dose Verification for Stereotactic Body Radiation Therapy Patients

    SciTech Connect (OSTI)

    Asuni, G; Beek, T van; Van Utyven, E; McCowan, P; McCurdy, B.M.C.

    2014-08-15

    Radical treatment techniques such as stereotactic body radiation therapy (SBRT) are becoming popular and they involve delivery of large doses in fewer fractions. Due to this feature of SBRT, a high-resolution, pre-treatment dose verification method that makes use of a 3D patient representation would be appropriate. Such a technique will provide additional information about dose delivered to the target volume(s) and organs-at-risk (OARs) in the patient volume compared to 2D verification methods. In this work, we investigate an electronic portal imaging device (EPID) based pre-treatment QA method which provides an accurate reconstruction of the 3D-dose distribution in the patient model. Customized patient plans are delivered ‘in air’ and the portal images are collected using the EPID in cine mode. The images are then analysed to determine an estimate of the incident energy fluence. This is then passed to a collapsed-cone convolution dose algorithm which reconstructs a 3D patient dose estimate on the CT imaging dataset. To date, the method has been applied to 5 SBRT patient plans. Reconstructed doses were compared to those calculated by the TPS. Reconstructed mean doses were mostly within 3% of those in the TPS. DVHs of target volumes and OARs compared well. The Chi pass rates using 3%/3mm in the high dose region are greater than 97% in all cases. These initial results demonstrate clinical feasibility and utility of a robust, efficient, effective and convenient pre-treatment QA method using EPID. Research sponsored in part by Varian Medical Systems.

  16. A High-Performance VLSI Architecture for Advanced Encryption Standard (AES) Algorithm

    E-Print Network [OSTI]

    Mohanty, Saraju P.

    A High-Performance VLSI Architecture for Advanced Encryption Standard (AES) Algorithm Naga M technology resulted in a throughput of 232Mbps for iterative architecture and 1.83Gbps for pipelining implementation. The proposed architecture is optimized for high throughput in terms of the encryption

  17. Asymptotic Throughput of the FED-STD-1052 Data Link Protocol Eric E. Johnson

    E-Print Network [OSTI]

    Johnson, Eric E.

    Asymptotic Throughput of the FED-STD-1052 Data Link Protocol Eric E. Johnson New Mexico State. · Diurnal and seasonal variations in usable channels from hour to hour and day to day. · Solar cycle effects

  18. Beyond Proportional Fairness: A Resource Biasing Framework for Shaping Throughput Profiles in

    E-Print Network [OSTI]

    Belding-Royer, Elizabeth M.

    Beyond Proportional Fairness: A Resource Biasing Framework for Shaping Throughput Profiles of resources. While proportional fair allocation provides a significant improvement, we point out here connections than max-min fair or proportional fair resource allocations, with minimal impact

  19. Throughput Performance of Quantized Proportional Fair Scheduling with Adaptive Modulation and

    E-Print Network [OSTI]

    Ishizaki, Fumio

    Throughput Performance of Quantized Proportional Fair Scheduling with Adaptive Modulation where QPF (Quantized Proportional Fair) scheduling and AMC (adaptive modulation and coding) scheme condition [11]. To solve this unfair problem, the proportional fair (PF) scheduling was proposed where

  20. Throughput Optimal Scheduling in the Presence of Heavy-Tailed Traffic

    E-Print Network [OSTI]

    Jagannathan, Krishna Prasanna

    We investigate the tail behavior of the steady-state queue occupancies under throughput optimal scheduling in the presence of heavy-tailed traffic. We consider a system consisting of two parallel queues, served by a single ...

  1. Map Task Scheduling in MapReduce with Data Locality: Throughput and Heavy-Traffic Optimality

    E-Print Network [OSTI]

    Zhang, Junshan

    is throughput optimal and the outer bound coincides with the actual capacity region. Further, we study in a distributed and parallel fashion, and has been widely used in practice, including Google, Yahoo!, Facebook

  2. SU-E-J-149: Establishing the Relationship Between Pre-Treatment Lung Ventilation, Dose, and Toxicity Outcome

    SciTech Connect (OSTI)

    Mistry, N; D'Souza, W; Sornsen de Koste, J; Senan, S

    2014-06-01

    Purpose: Recently, there has been an interest in incorporating functional information in treatment planning especially in thoracic tumors. The rationale is that healthy lung regions need to be spared from radiation if possible to help achieve better control on toxicity. However, it is still unclear whether high functioning regions need to be spared or have more capacity to deal with the excessive radiation as compared to the compromised regions of the lung. Our goal with this work is to establish the tools by which we can establish a relationship between pre-treatment lung function, dose, and radiographic outcomes of lung toxicity. Methods: Treatment planning was performed using a single phase of a 4DCT scan, and follow-up anatomical CT scans were performed every 3 months for most patients. In this study, we developed the pipeline of tools needed to analyze such a large dataset, while trying to establish a relationship between function, dose, and outcome. Pre-treatment lung function was evaluated using a recently published technique that evaluates Fractional Regional Ventilation (FRV). All images including the FRV map and the individual follow-up anatomical CT images were all spatially matched to the planning CT using a diffusion based Demons image registration algorithm. Change in HU value was used as a metric to capture the effects of lung toxicity. To validate the findings, a radiologist evaluated the follow-up anatomical CT images and scored lung toxicity. Results: Initial experience in 1 patient shows a relationship between the pre-treatment lung function, dose and toxicity outcome. The results are also correlated to the findings by the radiologist who was blinded to the analysis or dose. Conclusion: The pipeline we have established to study this enables future studies in large retrospective studies. However, the tools are dependent on the fidelity of 4DCT reconstruction for accurate evaluation of regional ventilation. Patent Pending for the technique presented in this work to evaluate FRV incorporating mass correction.

  3. Influence of mechanical-biological waste pre-treatment methods on the gas formation in landfills

    SciTech Connect (OSTI)

    Bockreis, A. [Technische Universitaet Darmstadt, Darmstadt University of Technology, Institute for Water Supply and Groundwater Protection, Wastewater Technology, Waste Management, Industrial Material Flows and Environmental Planning (Institute WAR), Chair of Waste Management and Waste Technology, Darmstadt (Germany)]. E-mail: a.bockreis@iwar.tu-darmstadt.de; Steinberg, I. [Technische Universitaet Darmstadt, Darmstadt University of Technology, Institute for Water Supply and Groundwater Protection, Wastewater Technology, Waste Management, Industrial Material Flows and Environmental Planning (Institute WAR), Chair of Waste Management and Waste Technology, Darmstadt (Germany)

    2005-07-01

    In order to minimise emissions and environmental impacts, only pre-treated waste should be disposed of. For the last six years, a series of continuous experiments has been conducted at the Institute WAR, TU Darmstadt, in order to determine the emissions from pre-treated waste. Different kinds of pre-treated waste were incubated in several reactors and various data, including production and composition of the gas and the leachate, were collected. In this paper, the interim results of gas production and the gas composition from different types of waste after a running time of six years are presented and discussed.

  4. Effect of Acid, Alkali, and Steam Explosion Pretreatments on Characteristics of Bio-Oil Produced from Pinewood

    SciTech Connect (OSTI)

    Wang, Hui; Srinivasan, Radhakrishnan; Yu, Fei; Steele, Philip; Li, Qi; Mitchell, Brian

    2011-06-21

    Bio-oil produced from pinewood by fast pyrolysis has the potential to be a valuable substitute for fossil fuels. Pretreatment prior to the fast pyrolysis process has been shown to alter the structure and chemical composition of biomass. To determine the influence of biomass pretreatments on bio-oil produced during fast pyrolysis, we tested three pretreatment methods: dilute acid, dilute alkali, and steam explosion. Bio-oils were produced from untreated and pretreated pinewood feedstocks in an auger reactor at 450 C. The bio-oils�¢���� physical properties including pH, water content, acid value, density, viscosity, and heating value were measured. Chemical characteristics of the bio-oils were determined by gas chromatographymass spectrometry. Results showed that bio-oil yield and composition were influenced by biomass pretreatment. Of the three pretreatment methods, 1%H2SO4 pretreatment resulted in the highest bio-oil yield and best bio-oil quality.

  5. A Pre-treatment Model for Ethanol Production Using a Colorimetric...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Pre-treatment Model for Ethanol Production Using a Colorimetric Analysis of Starch Solutions AUTHORS: Eric Benson and Chris Ederer E-mail Addresses: eric.benson@theloganschool.o...

  6. Cluster Computing: High-Performance, High-Availability, and High-Throughput Processing on a Network of Computers

    E-Print Network [OSTI]

    Buyya, Rajkumar

    Laboratory Dept. of Computer Science and Software Engineering The University of Melbourne, Australia {csyeo, raj}@cs.mu.oz.au 2 Parallel and Distributed Systems Laboratory Department of Computer Science for cluster computing was developed in the 1960s by IBM as an alternative of linking large mainframes

  7. Development of an improved sodium titanate for the pretreatment of nuclear waste at the Savannah River Site

    SciTech Connect (OSTI)

    Hobbs, D.T.; Poirier, M.R.; Barnes, M.J.; Peters, T.B.; Fondeur, F.F.; Thompson, M.E.; Fink, S.D. [Savannah River National Laboratory, Westinghouse Savannah River Company, Aiken, SC (United States); Nyman, M.D. [Sandia National Laboratories, Albuquerque, NM (United States)

    2008-07-01

    High-level nuclear waste produced from fuel reprocessing operations at the Savannah River Site (SRS) requires pretreatment to remove Cs-137, Sr-90 and alpha-emitting radionuclides (i.e., actinides) prior to disposal onsite as low level waste. Separation processes planned at SRS include sorption of Sr-90 and alpha-emitting radionuclides onto monosodium titanate (MST) and caustic side solvent extraction, for Cs-137 removal. The MST and separated Cs-137 will be encapsulated into a borosilicate glass wasteform for eventual entombment at the federal repository. The predominant alpha-emitting radionuclides in the highly alkaline waste solutions include plutonium isotopes Pu-238, Pu-239 and Pu-240. This paper describes recent results to produce an improved sodium titanate material that exhibits increased removal kinetics and capacity for Sr-90 and alpha-emitting radionuclides compared to the baseline MST material. (authors)

  8. SU-E-CAMPUS-T-02: Can Pre-Treatment 4DCT-Based Motion Margins Estimates Be Trusted for Proton Radiotherapy?

    SciTech Connect (OSTI)

    Seco, J; Koybasi, O; Mishra, P; James, S St.; Lewis, J

    2014-06-15

    Purpose: Radiotherapy motion margins are generated using pre-treatment 4DCT data. The purpose of this study is to assess if pre-treatment 4DCT is sufficient in proton therapy to provide accurate estimate of motion margins. A dosimetric assessment is performed comparing pre-treatment margins with daily-customized margins. Methods: Gold fiducial markers implanted in lung tumors of patients were used to track the tumor. A spherical tumor of diameter 20 mm is inserted into a realistic digital respiratory phantom, where the tumor motion is based on real patient lung tumor trajectories recorded over multiple days. Using “Day 1” patient data, 100 ITVs were generated with 1 s interval between consecutive scan start times. Each ITV was made up by the union of 10 tumor positions obtained from 6 s scan time. Two ITV volumes were chosen for treatment planning: ITVmean-? and ITVmean+?. The delivered dose was computed on i) 10 phases forming the planning ITV (“10-phase” - simulating dose calculation based on 4DCT) and ii) 50 phantoms produced from 100 s of data from any other day with tumor positions sampled every 2 s (“dynamic” - simulating the dose that would actually be delivered). Results: For similar breathing patterns between “Day 1” and any other “Day N(>1)”, the 95% volume coverage (D95) for “dynamic” case was 8.13% lower than the “10-phase” case for ITVmean+?. For breathing patterns that were very different between “Day 1” and any other “Day N(>1)”, this difference was as high as 24.5% for ITVmean-?. Conclusion: Proton treatment planning based on pre-treatment 4DCT can lead to under-dosage of the tumor and over-dosage of the surrounding tissues, because of inadequate estimate of the range of motion of the tumor. This is due to the shift of the Bragg peak compared to photon therapy in which the tumor is surrounded by an electron bath.

  9. A Deeply Pipelined CABAC Decoder for HEVC Supporting Level 6.2 High-tier Applications

    E-Print Network [OSTI]

    Chen, Yu-Hsin

    High Efficiency Video Coding (HEVC) is the latest video coding standard that specifies video resolutions up to 8K Ultra-HD (UHD) at 120 fps to support the next decade of video applications. This results in high-throughput ...

  10. SU-E-T-65: Characterization of a 2D Array for QA and Pretreatment Plan Verification

    SciTech Connect (OSTI)

    Anvari, A; Aghamiri, S; Mahdavi, S; Alaei, P

    2014-06-01

    Purpose: The OCTAVIUS detector729 is a 2D array of 729 air vented cubic plane parallel ion chambers used for pretreatment verification and QA. In this study we investigated dosimetric characteristics of this system for clinical photon beam dosimetry. Methods: Detector performance evaluation included determination of the location of the effective point of measurement (EPM), sensitivity, linearity, and reproducibility of detector response, as well as output factor, dose rate, and source to surface distance (SSD) dependence. Finally, assessment of wedge modulated fields was carried out. All the evaluations were performed five times for low and high photon energies. For reference measurements, a 0.6 cc ionization chamber was used. Data analysis and comparison of the OCTAVIUS detector with reference ion chamber data was performed using the VeriSoft patient plan verification software. Results: The reproducibility and stability of the measurements are excellent, the detector showed same signal with a maximum deviation of less than 0.5% in short and long term. Results of sensitivity test showed same signal with a maximum deviation of approximately 0.1%. As the detector 729 response is linear with dose and dose rate, it can be used for the measurement at regions of high dose gradient effectively. The detector agrees with the ionization chamber measurement to within 1% for SSD range of 75 to 125 cm. Also, its measured wedge modulated profiles matched very well with ion chamber dose profiles acquired in a water tank. Conclusions: As the response of the detector 729 is linear with dose and dose rate, it can be used for the measurements in the areas of dose gradients effectively. Based on the measurements and comparisons performed, this system is a reliable and accurate dosimeter for QA and pretreatment plan verification in radiotherapy.

  11. Maximizing the throughput of large ad hoc wireless networks

    E-Print Network [OSTI]

    Hua, Y; Huang, Y; Garcia-Luna-Aceves, J J

    2006-01-01

    network consists of wireless routers that are highly coop-point for every m wireless routers in a 2-D This capacitylinks are used for wireless routers). tion rates of a small

  12. Microfabricated arrays for high-throughput screening of cellular response to cyclic substrate deformation

    E-Print Network [OSTI]

    Sun, Yu

    and pathobiology. Introduction The large number of factors in the cellular milieu that can impact biological to other stimuli, including matrix proteins,12 chemical cues,13 and genetic therapies.14 In some cases

  13. SNP Calling Using Genotype Model Selection on High-Throughput Sequencing Data

    E-Print Network [OSTI]

    Murillo, Gabriel Hiroshi

    2012-01-01

    microorganisms or microbes has been shown to be of increasing importance not only for human and domestic animal pathogen

  14. Multi-Channel and Multi-Rate Adaptation for High-Throughput Wireless Networks

    E-Print Network [OSTI]

    Nguyen, Duy Duc

    2012-01-01

    layer. Unlike wired networks, wireless networks have to deal2 Background Wireless Networks Wireless technology is allWLAN), wireless mesh networks, wireless metropolitan area

  15. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    walls, a small leak, or outgassing from the sample. Theyou have found the leak. Outgassing samples are the mostheld for 24 hrs or until outgassing was determined complete

  16. Algorithms and tools for high-throughput geometry-based analysis...

    Office of Scientific and Technical Information (OSTI)

    (SC-22) Country of Publication: United States Language: English Subject: membrane, carbon capture, materials and chemistry by design, synthesis (novel materials), synthesis...

  17. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    Microporous and Mesoporous Materials 2001, (29) Occelli, M.Microporous and Mesoporous Materials 2012, (170) Ben, T. ;Zhou, H. -C. Chemistry of Materials 2010, (172) Yuan, D. ;

  18. A truly unique high throughput, micro-volume protein analysis and characterisation system.

    E-Print Network [OSTI]

    Lebendiker, Mario

    and formulation studies. 2. Measurements with conventional analytical instrumentation are often slow and labour in the later stages of development.This can increase the risk of costly late stage problems or failure

  19. HIGH-THROUGHPUT/COMBINATORIAL TECHNIQUES IN HYDROGEN STORAGE MATERIALS R&D WORKSHOP

    E-Print Network [OSTI]

    , academia, and national laboratories. The objectives of this meeting were to: · Assess the potential is solution-based with solids handling similar to proven combinatorial methods. Exploration of new structures-ray scattering (SAXS) to determine pore size (needs development), hydrogen uptake me

  20. Development of High Throughput Process for Constructing 454 Titanium and Illumina Libraries

    SciTech Connect (OSTI)

    Deshpande, Shweta; Hack, Christopher; Tang, Eric; Malfatti, Stephanie; Ewing, Aren; Lucas, Susan; Cheng, Jan-Fang

    2010-05-28

    We have developed two processes with the Biomek FX robot to construct 454 titanium and Illumina libraries in order to meet the increasing library demands. All modifications in the library construction steps were made to enable the adaptation of the entire processes to work with the 96-well plate format. The key modifications include the shearing of DNA with Covaris E210 and the enzymatic reaction cleaning and fragment size selection with SPRI beads and magnetic plate holders. The construction of 96 Titanium libraries takes about 8 hours from sheared DNA to ssDNA recovery. The processing of 96 Illumina libraries takes less time than that of the Titanium library process. Although both processes still require manual transfer of plates from robot to other work stations such as thermocyclers, these robotic processes represent about 12- to 24-folds increase of library capacity comparing to the manual processes. To enable the sequencing of many libraries in parallel, we have also developed sets of molecular barcodes for both library types. The requirements for the 454 library barcodes include 10 bases, 40-60percent GC, no consecutive same base, and no less than 3 bases difference between barcodes. We have used 96 of the resulted 270 barcodes to construct libraries and pool to test the ability of accurately assigning reads to the right samples. When allowing 1 base error occurred in the 10 base barcodes, we could assign 99.6percent of the total reads and 100percent of them were uniquely assigned. As for the Illumina barcodes, the requirements include 4 bases, balanced GC, and at least 2 bases difference between barcodes. We have begun to assess the ability to assign reads after pooling different number of libraries. We will discuss the progress and the challenges of these scale-up processes.

  1. High throughput analysis of proteome perturbations induced by radiation, radiomitigators and chemotherapeutics

    E-Print Network [OSTI]

    Erde, Jonathan

    2012-01-01

    in five pathways of glutamate fermentation. Appl MicrobiolH. A. Two pathways of glutamate fermentation by anaerobic

  2. Automated high-throughput flow-through real-time diagnostic system

    DOE Patents [OSTI]

    Regan, John Frederick

    2012-10-30

    An automated real-time flow-through system capable of processing multiple samples in an asynchronous, simultaneous, and parallel fashion for nucleic acid extraction and purification, followed by assay assembly, genetic amplification, multiplex detection, analysis, and decontamination. The system is able to hold and access an unlimited number of fluorescent reagents that may be used to screen samples for the presence of specific sequences. The apparatus works by associating extracted and purified sample with a series of reagent plugs that have been formed in a flow channel and delivered to a flow-through real-time amplification detector that has a multiplicity of optical windows, to which the sample-reagent plugs are placed in an operative position. The diagnostic apparatus includes sample multi-position valves, a master sample multi-position valve, a master reagent multi-position valve, reagent multi-position valves, and an optical amplification/detection system.

  3. Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

    E-Print Network [OSTI]

    Cai, Long

    shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We strength in other fields9. How- ever, SAXS use has sharply increased with advances in synchrotron X,10,11. Notably, SAXS analyses can build on and be combined with other results to test experimental hypotheses

  4. High-Throughput Assay of 9 Lysosomal Enzymes for Newborn Screening

    E-Print Network [OSTI]

    Gelb, Michael

    transfers before injection into a dual-column HPLC equipped with switching valves to direct the flow for LSD expanded newborn screening has passed in several states. Newborn screening for Pompe and Fabry

  5. High-throughput, single-worm tracking and analysis in Caenorhabditis elegans

    E-Print Network [OSTI]

    Yemini, Eviatar

    2013-01-08

    orifices at the mouth, anus, and, for hermaphrodites, at the vulva as well. Males have special mating adaptations at their tail. At low magnification, as would be expected, C. elegans have a simple worm-like morphology and the tail has a slightly sharper... shapes (as defined by bending angles along the worm body) 15. The most salient and commonly explored behavior in C. elegans is locomotion. Locomotion is powered by 95 muscles separated into four longitudinal quadrants 16. These quadrants represent left...

  6. Discovery of BRD4 bromodomain inhibitors by fragment-based high-throughput docking

    E-Print Network [OSTI]

    Caflisch, Amedeo

    % as only 24 putative inhibitors, from an initial library of about 9 million molecules, were tested in vitro efficiency (LE), defined as the ratio of the free energy of binding to the number of non-hydrogen atoms.11 Previously, we have developed a fragment-based in silico proce- dure called ALTA (Anchor-based Library

  7. Development and Application of a High Throughput Methodology to Characterize and Formulate Protein-Based Therapeutics

    E-Print Network [OSTI]

    Hu, Lei

    2012-12-31

    concentration and excipients on stability, as well as their effects on solubility.8 Although not addressed in the present work, other studies including photostability, agitation, and freeze-thaw cycling are also usually performed to evaluate the effect...

  8. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    parameters for MOF-5, Cu-BTC and ZIF-8 were from universalMOF materials, MOF-5 129 and Cu-BTC 130 , again at roomFor methane adsorption in Cu-BTC, however, WDA-Y reproduces

  9. Droplet microfluidics for high-throughput biological assays Mira T. Guo,a

    E-Print Network [OSTI]

    developed over the past several decades have greatly impacted medical research, therapeutics signal-to-noise ratios restrict the possible targets of medical diagnostics, and thus limit their medical sensitivities by using water-in-oil emulsion droplets to compartmentalize reactants into picolitre volumes,3

  10. Integrated microfluidic device for single-cell high throughput screening in dynamic gene expression analysis

    E-Print Network [OSTI]

    Hui, Lawrence Kwan Yeung

    2008-01-01

    the resulting fluid flow lies in the laminar flow regime andfluid flow at the microflu- idic scale was strictly laminar andof laminar flow is that mixing of two conjoin- ing fluids is

  11. A high-throughput three-dimensional cell migration assay for toxicity screening

    E-Print Network [OSTI]

    Diseases, University of Texas Health Science Center, Houston, TX 77030 USA. There is a growing demand,19­21 . These nanoparticles are nontoxic and do not induce an inflammatory cytokine (IL-6, IL-8) response by cells22 they interact and aggregate with each other to form larger 3D cultures. This method has been shown to induce

  12. Inch-Scale High Throughput Metrology of Graphene and Patterned Graphene Oxide

    E-Print Network [OSTI]

    Pleskot, Dennis

    2013-01-01

    Strength of Monolayer Graphene Science 2008 321, 385-388. 5.Novoselov, K. S. The rise of graphene Nature 2007 6 183-191.A. Thermal properties of graphene and nanostructured carbon

  13. Analysis of Genomic Rearrangements in Cancer from High Throughput Sequencing Data

    E-Print Network [OSTI]

    Ballinger, Tracy Jean

    2015-01-01

    Breakage fusion bridge (BFB) cycles are thought to play achromosome, a combination of BFB and chromothripsis events,they are not explainable by BFB cycles or chromothripsis

  14. A high-throughput in vivo micronucleus assay for genome instability screening in mice

    E-Print Network [OSTI]

    Balmus, Gabriel; Karp, Natasha A.; Ng, Bee Ling; Jackson, Stephen P.; Adams, David J.; McIntyre, Rebecca E.

    2014-12-31

    and detection of molecules involved in the response to exogenous DNA damaging agents DNA damage response (DDR) defects can be revealed by treating mice that show normal spontaneous micronucleus frequencies with genotoxic agents. Upon treatment, micronuclei... or for the identification of deficiencies that are only apparent, or amplified, in response to DDR challenge because they are only necessary for the response to exogenous DNA damaging 640 agents rather than endogenous DNA damaging agents. This procedure will enable...

  15. High-throughput single-molecule fluorescence spectroscopy using parallel detection

    E-Print Network [OSTI]

    Michalet, Xavier

    -molecule imaging). In this section, we briefly discuss the technique and data analysis used in standard solution-based , S. Tisad , F. Zappad , S. Covad , S. Weissa a Dept of Chemistry & Biochemistry, Los Angeles, CA, USA 90095; b Nesher Technologies, Los Angeles, CA; c Dept of Physics & Astronomy, UCLA, Los Angeles, CA

  16. Multi-Channel and Multi-Rate Adaptation for High-Throughput Wireless Networks

    E-Print Network [OSTI]

    Nguyen, Duy Duc

    2012-01-01

    collisions, and natural radio waves phenomena. This isphenomena associated to radio wave propagation are the keyinformation bit into radio waves. The wireless medium is

  17. The Development of a High-throughput Microdroplet Bioreactor Device for Microbial Studies 

    E-Print Network [OSTI]

    Guzman, Adrian

    2012-10-19

    . These methods often produce much larger forces that are capable of overcoming the additional surface stability added by surfactant usage. Single target droplet merging can be accomplished using a laser in a variety of ways including cavitational bubble... induction, thermal localized heating, or surface convective heating. In the case of cavitational bubble induction, a bubble containing the vapor of the dispersed phase is induced using a localized laser heating source [43]. The droplet then expands...

  18. High-throughput metal nanoparticle catalysis by pulsed laser ablation Selim Senkan a,*, Michael Kahn a

    E-Print Network [OSTI]

    Senkan, Selim M.

    -PLA were also screened for their catalytic activities and selectivities for the partial oxidation for catalytic applications. Catalytic materials containing Rh, bimetallic Rh/Pt and trimetallic Rh of propylene. In less than a day, over 40 different catalytic materials of nanoparticles supported on g-Al2O3

  19. Ultra-high Throughput Real-time Instruments for Capturing Fast Signals and Rare Events

    E-Print Network [OSTI]

    Buckley, Brandon Walter

    2013-01-01

    using a syringe pump, at a fixed volumetric rate. The fluidat a fixed volumetric rate using a syringe pump. CHAPTER 9

  20. Integrated microfluidic device for single-cell high throughput screening in dynamic gene expression analysis

    E-Print Network [OSTI]

    Hui, Lawrence Kwan Yeung

    2008-01-01

    P.J. , & Lee, L.P. , Microfluidics-based systems biology.T.M. & Quake, S.R. , Microfluidics: Fluid physics at theand applications of microfluidics in biology. Annu Rev

  1. High-throughput microfluidic living cell arrays for spatiotemporal gene expression profiling

    E-Print Network [OSTI]

    King, Kevin R. (Kevin Robert), 1976-

    2008-01-01

    The cellular microenvironment is remarkably complex. In the small space near each cell, growth factors are liberated from extracellular matrix, cytokines are secreted from neighboring cells, and hormones arrive from distant ...

  2. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    rheology through microfluidics,” Advanced Materials, vol.Berthier and P. Silberzan, Microfluidics for Biotechnology.A. P. Lee, “Droplet microfluidics,” Lab on a Chip, vol. 8,

  3. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    the compressed natural gas (CNG) at the same temperature andcompressed natural gas tank (CNG). According to this table,Compressed Natural Gas (CNG) 136 , involve either cryogenic

  4. High throughput analysis of proteome perturbations induced by radiation, radiomitigators and chemotherapeutics

    E-Print Network [OSTI]

    Erde, Jonathan

    2012-01-01

    research and biohydrogen production development. Chapters 6,fossil fuel-free biohydrogen production, and for proteomicbe important for biohydrogen production 1 . It is capable of

  5. High throughput 3D optical microscopy : from image cytometry to endomicroscopy

    E-Print Network [OSTI]

    Choi, Heejin

    2014-01-01

    Optical microscopy is an imaging technique that allows morphological mapping of intracellular structures with submicron resolution. More importantly, optical microscopy is a technique that can readily provide images with ...

  6. Treatability studies on different refinery wastewater samples using high-throughput microbial electrolysis cells (MECs)

    E-Print Network [OSTI]

    , 2005), brewery (Feng et al., 2008), animal (Min et al., 2005) and paper recycling wastewaters (Huang

  7. Openly Accessible Microfluidic Liquid Handlers for Automated High-Throughput Nanoliter Cell Culture

    E-Print Network [OSTI]

    Huang, Yanyi

    addressability. Moreover, the additional accessories for micropumps and valves, including pressurized gas supply to drug screening2 and genomic profiling.3 These large-scale experiments would naturally prefer minute such as valves, pumps, and necessary bypassing channels, it is still chall- enging to realize individual

  8. SUPPLEMENTARY DATA DNAshape: a method for the high-throughput prediction

    E-Print Network [OSTI]

    Rohs, Remo

    central base pairs (underlined in Supplementary Table S2), and (C-D) comparisons with crystal structures 3 in Figure 2 include data from X-ray crystallography (1bna, 2bna, 355d, 428d, 455d, 1dou, 1fq2, and 1jgr

  9. High-Throughput Automated Luminescent Magnetic Particle-Based Immunoassay to Monitor

    E-Print Network [OSTI]

    Hammock, Bruce D.

    to Pyrethroid Insecticides Ki Chang Ahn, Pete Lohstroh,,# Shirley J. Gee, Nancy A. Gee, Bill Lasley, and Bruce D, University of California, Davis, California 95616 We have developed a sensitive, automated, competitive

  10. High throughput lessons from the LHC experience.Johnston.TNC2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    signal transport, layer 2 transport, data transport (TCP is still the norm), operating system evolution, data movement and management techniques and software, and increasing...

  11. High throughput analysis of proteome perturbations induced by radiation, radiomitigators and chemotherapeutics

    E-Print Network [OSTI]

    Erde, Jonathan

    2012-01-01

    expression levels in Normal LCLs, 24 hours after treatmenthuman lymphoblastoid cell lines (LCLs) exposed to ionizingresponse components in Normal LCLs, while thrombin signaling

  12. Geochip: A high throughput genomic tool for linking community structure to functions

    E-Print Network [OSTI]

    Van Nostrand, Joy D.

    2009-01-01

    mentioned previously, probe design criteria are important inis determining probe design criteria, as this will greatlyof oligonucleotide probe design criteria. Appl Environ

  13. LabFlow1: a Database Benchmark for HighThroughput Workflow Management ?

    E-Print Network [OSTI]

    Bonner, Anthony

    work­ flow management systems (WFMS). An important component of many WFMSs is a DBMS for keeping track of workflow activity. This DBMS maintains an audit trail, or event history, that records the results of each evolving business environment. Since the database schema depends on the work­ flow, the DBMS must also

  14. Database Requirements for Workflow Management in a HighThroughput Genome Laboratory 1

    E-Print Network [OSTI]

    Bonner, Anthony

    and operationally efficient. A key component of this software is a database management system (DBMS) for controlling and tracking workflow activity. This DBMS maintains a record of what happened in the laboratory. This record the requirements of this DBMS. The discussion centers on LabFlow­1 [3, 2], a recently developed database benchmark

  15. LabFlow1: a Database Benchmark for HighThroughput Workflow Management \\Lambda

    E-Print Network [OSTI]

    Bonner, Anthony

    called workflow management systems (WFMS). An important component of many WFMSs is a DBMS for keeping track of workflow activity. This DBMS maintains an audit trail, or event history, that records a rapidly evolving business environment. Since the database schema depends on the workflow, the DBMS must

  16. Improved High Maximum Stable Throughput FCFS Tree Algorithms with Interference Cancellation

    E-Print Network [OSTI]

    . Although all these random access channels rely on the well known binary exponential backoff (BEB) algorithm also strongly outperform the class of back- off algorithms (including the BEB) in terms of their maxi

  17. Inertio-elastic focusing of bioparticles in a microchannel at ultra-high throughput

    E-Print Network [OSTI]

    Lim, Eugene J. (Eugene Jungsud), 1980-

    2014-01-01

    Many biological and industrial fluids are filled with micro-scale particles that can serve as "state markers" for real-world issues, such as human health and public infrastructure. In order to extract this valuable information ...

  18. Marrying microfluidics and microwells for parallel, high-throughput single-cell genomics

    E-Print Network [OSTI]

    Hughes, Travis K.

    An innovative, microwell-based platform for single-cell RNA sequencing (RNA-seq) combines cost efficiency, scalability and parallelizability, and will enable many new avenues of biological inquiry.

  19. High-throughput Microfluidics and Ultrafast Optics for in Vivo Compound/Genetic Discoveries

    E-Print Network [OSTI]

    Rohde, Christopher Benjamin

    Therapeutic treatment of spinal cord injuries, brain trauma, stroke, and neurodegenerative diseases will greatly benefit from the discovery of compounds that enhance neuronal regeneration following injury. We previously ...

  20. Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening

    SciTech Connect (OSTI)

    Xu, Hongbo; Chen, Xiaohong; Huang, Junwei [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Deng, Weiwei [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China)] [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Zhong, Qi [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Yue, Changli [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China)] [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China); Wang, Pingzhang, E-mail: wangpzh@bjmu.edu.cn [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China) [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China); Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Huang, Zhigang, E-mail: enthuangzhigang@sohu.com [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)

    2013-06-21

    Highlights: •A novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors was defined. •GPR65 was identified to induce the MMP3 expression. •GPR65 mediated MMP induction under acidic conditions. •AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. •GPR65 overexpression can accelerate the invision of A549 cells. -- Abstract: Matrix metalloproteinases (MMPs) are over-expressed in nearly all cancers. To study novel regulatory factors of MMP expression in head and neck cancer (HNC), we screened a total of 636 candidate genes encoding putative human transmembrane proteins using MMP promoter reporter in a dual luciferase assay system. Three genes GPR65, AXL and TNFRSF10B dramatically activated the induction of MMP3 expression. The induction of MMP expression by GPR65 was further confirmed in A549 and/or FaDu cells. GPR65 mediated MMP induction under acidic conditions. The AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. Moreover, the A549 cells infected by recombinant adenovirus of GPR65 showed accelerated cell invasion. In conclusion, we validate that GPR65 is vital regulatory genes upstream of MMP3, and define a novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors.

  1. Lensfree Optical Tomography for High-Throughput 3D Imaging on a Chip

    E-Print Network [OSTI]

    ISIKMAN, SERHAN OMER

    2012-01-01

    microscope with light-emitting diode illumination. Opt.source such as a light emitting diode (LED). The fundamentalchip microscope, 24 light-emitting diodes (LEDs - each with

  2. High Throughput Ab initio Modeling of Charge Transport for Bio-Molecular-Electronics

    E-Print Network [OSTI]

    Bruque, Nicolas A.

    2009-01-01

    DNA-Based Molecular Electronics. vol. 725. New York: AIP,Heath, “Whence molecular electronics? ” Science, vol. 306,model,” J. Computational Electronics, vol. 6, no. 4, pp.

  3. WHAM: A High-throughput Sequence Alignment Method Yinan Li Allison Terrell Jignesh M. Patel

    E-Print Network [OSTI]

    Willett, Rebecca

    -1-4503-0661-4/11/06 ...$10.00. only a small portion (called a "read") of the genome. The human genome project delivered its anniversary of the sequencing of the first human genome [1, 21]. This key scientific discovery has been to drug discovery and drug design for personal- ized medicine. A crucial part of this first human genome

  4. SNP Calling Using Genotype Model Selection on High-Throughput Sequencing Data

    E-Print Network [OSTI]

    Murillo, Gabriel Hiroshi

    2012-01-01

    Institute. The human genome project comple- tion: Frequentlycommunity, the Human Genome Project (HGP) was finished inmain goals of the human genome project was to create a human

  5. A microfluidics approach towards high-throughput pathogen removal from blood using margination

    E-Print Network [OSTI]

    Hou, Han Wei

    Sepsis is an adverse systemic inflammatory response caused by microbial infection in blood. This paper reports a simple microfluidic approach for intrinsic, non-specific removal of both microbes and inflammatory cellular ...

  6. High-throughput gene expression profiling of memory differentiation in primary human T cells

    E-Print Network [OSTI]

    Angelosanto, Jill

    Background: The differentiation of naive T and B cells into memory lymphocytes is essential for immunity to pathogens. Therapeutic manipulation of this cellular differentiation program could improve vaccine efficacy and ...

  7. High-throughput experimental and computational tools for exploring immunity and the microbiome

    E-Print Network [OSTI]

    Papa, Eliseo

    2012-01-01

    Humans live in association with trillions of microbes and yet we know remarkably little about their symbiotic relationship. The role these microorganisms have in humans has been characterized only in the case of few bacteria ...

  8. Editor's Choice The AFLOW standard for high-throughput materials science calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    c , Michael J. Mehl d , Gus Hart e , Marco Buongiorno Nardelli f , Stefano Curtarolo g, a Department calculation and communication protocols. Following these guidelines would promote optimal use of the results

  9. Development of a high throughput 3D perfused liver tissue bioreactor

    E-Print Network [OSTI]

    Inman, Samuel Walker

    2006-01-01

    This thesis describes the development of a device designed for culturing liver tissue in a 3D perfused environment. Cells form tissue inside miniature channels of a scaffold, and the tissue is perfused with culture medium ...

  10. High-throughput approaches to sourcing of human hepatocytes for cell-based therapies

    E-Print Network [OSTI]

    Shan, Jing (Jing Meghan)

    2014-01-01

    Chronic liver disease affects more than 500 million people worldwide. The only therapy shown to directly prevent mortality is organ transplantation. However, there is growing discrepancy between supply and demand of ...

  11. A High-throughput Screening Approach Identifies Selective Regulators of Il12b Transcription

    E-Print Network [OSTI]

    Doty, Kevin Richard

    2012-01-01

    antibody, in patients with psoriasis: 76-week results from a1994). T cells involved in psoriasis vulgaris belong to theBoehncke, W. -H. (2005). Psoriasis. The New England journal

  12. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    3.1.2 Benefits of Thermoplastic Devices . . . . . . . . .temperatures for four thermoplastics. The pressure in eachto fabricate and test thermoplastic devices is presented.

  13. Supplemental Information Assessing the thermoelectric properties of sintered compounds via high-throughput ab initio calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    6277 orc oP12 12 0.50 1.59 1.82 6.00 0.54 0.38 0.47 0.77 4.28 0.09 4.76 7.98 Ag1I1 43435 cub cP2 2 0.04 2.17 2.19 2.94 2.47 3.10 3.25 Ag2Se1 15213 orc oP12 12 0.16 1.12 0.30 0.60 0.14 0.10 0.12 0.21 5.08 0.08 0.37 0.37 0.37 0.37 Al1As3Ca3 32727 orc oP28 28 0.72 1.89 0.84 1.23 1.72 0.32 0.94 3.89 2.06 1

  14. Operations capability improvement of a molecular biology laboratory in a high throughput genome sequencing center

    E-Print Network [OSTI]

    Vokoun, Matthew R. (Matthew Richard)

    2005-01-01

    The Broad Institute is a research collaboration of MIT, Harvard University and affiliated hospitals, and the Whitehead Institute for Biomedical Research. Its scientific mission is to "(1) create tools for genomic medicine ...

  15. High-Throughput Screen of Natural Product Libraries for Hsp90 Inhibitors

    E-Print Network [OSTI]

    Davenport, Jason; Blach, Maurie; Galam, Lakshmi; Girgis, Antwan; Hall, Jessica; Blagg, Brian S. J.; Matts, Robert L.

    2014-03-03

    with the report by Whitesell and co-workers that benzoquinone ansamycins, natural products isolated from the soil actinomycetes species Streptomyces hygroscopicus, were inhibitors of Hsp90 and not tyrosine kinases [1]. In 1997, the crystal structure of the Hsp90...) by Hsp90 [2]. Subsequently in 1998, radicicol, an antibiotic isolated from the mycoparasitic fungus Humicola fuscoatra, was found to bind similarly to the N-terminal domain of Hsp90 [3,4], and with the additional co-crystal structure in 1999, the road...

  16. SUPPLEMENTAL INFORMATION Treatability of Complex Effluents in High-Throughput and Bench Scale Microbial

    E-Print Network [OSTI]

    energy based on measured current, applied potential, and COD removal with various substrates in mini. The efficiency was calculated using only recovered hydrogen and combined hydrogen and methane, using the heat of combustion to calculate the energy contained in the gas. Figure S13. Open circuit gas production and COD

  17. An approach to simultaneously test multiple devices for high-throughput production of thin film electronics

    E-Print Network [OSTI]

    Kumar, A.; Flewitt, A. J.

    2015-07-28

    of transparent flexible thin-film transistors using amorphous oxide semiconductors.,” Nature, vol. 432, no. 7016, pp. 488–492, 2004. [2] E. Fortunato, P. Barquinha, and R. Martins, “Oxide semiconductor thin-film transistors: a review of recent advances... , M. Lavine, and R. Coontz, “Looking Beyond Silicon,” Science, vol. 327, no. 5973. pp. 1595–1595, 2010. [6] A. J. Flewitt, “Hydrogenated Amorphous Silicon Thin Film Transistors (a Si:H TFTs),” Handbook of Visual Display Technology-Springer, 2012...

  18. High-Throughput Single-Cell Manipulation in Brain Joseph D. Steinmeyer1

    E-Print Network [OSTI]

    Yanik, Mehmet Fatih

    . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which Award (R01 NS066352) and a Packard Award in Science and Engineering. J.D.S. was supported

  19. Some Clustering and Classification Problems in High-Throughput Metagenomics and Cheminformatics

    E-Print Network [OSTI]

    Tanaseichuk, Olga

    2013-01-01

    Gut Microbiome [43] and Acid Mine Drainage Biofilm [104].obtained from the acid mine drainage [104]. This dataset has

  20. Improving the Yeast Three-Hybrid System for High-Throughput Target Discovery

    E-Print Network [OSTI]

    Bailey, Kyle

    2011-05-27

    -protein interactions. The yeast three-hybrid system is an attractive alternative that uses genetic tools to screen for protein-small molecule interactions in cellulo. This thesis describes efforts to improve the utility of the yeast three-hybrid system to screen...

  1. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    of com- plex oil inlet shapes. (a) 2D extrusion to form aof complex oil inlet shapes. (a) 2D extrusion to form a pad.

  2. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    is a valid assumption. The viscosity of the oil was alsoassumption is often wrong, be- Table 4.2: Summary of measured viscosities from capillary measurement. Oil/

  3. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    Vehicles Concerns over the national energy security and global climate change are calling for the development of alternative

  4. Combined Catalysis and Optical Screening for High Throughput Discovery of Solar Fuels Catalysts

    E-Print Network [OSTI]

    Faraon, Andrei

    ,b a Joint Center for Artificial Photosynthesis, California Institute of Technology, Pasadena, California discovery effort within the Joint Center for Artificial Photosynthesis (JCAP, http

  5. High-throughput and long-term observation of compartmentalized biochemical oscillators

    E-Print Network [OSTI]

    Koshi Hasatani; Mathieu Leocmach; Anthony J. Genot; André Estévez-Torres; Teruo Fujii; Yannick Rondelez

    2015-11-04

    We report the splitting of an oscillating DNA circuit into $\\sim 700$ droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenomena - slow desynchronization between the compartments and kinematic spatial waves - and investigate their possible origin. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.

  6. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    tension versus time plot of peanut oil with varying weightshowing autofluorescence of peanut oil/SPAN80 mixture. Thetension versus time plot of peanut oil with varying weight

  7. Polymer microfluidic device for high-throughput single-cell encapsulation, lysis, and biological assay

    E-Print Network [OSTI]

    Brackbill, Timothy Peter

    2012-01-01

    3.5.1 Molding Attempts . . . . . . . . . . . . . . . . .3.8 Injection Molding of futureW. K. Schomburg, “Review on micro molding of ther- moplastic

  8. Multi-Channel and Multi-Rate Adaptation for High-Throughput Wireless Networks

    E-Print Network [OSTI]

    Nguyen, Duy Duc

    2012-01-01

    experiments include wireless cards and router with Atherosexperiments include wireless cards and router with Atherosvia wired or wireless connection to AP router. In both

  9. High Throughput Web Inspection System using Time-stretch Real-time Imaging

    E-Print Network [OSTI]

    Kim, Chanju

    2013-01-01

    successfully report a dark-field web inspection system withsample substrate for our dark-field web inspection systemweb inspection system exploiting time- stretch and utilizing the merits coming from dark-

  10. Analysis of Genomic Rearrangements in Cancer from High Throughput Sequencing Data

    E-Print Network [OSTI]

    Ballinger, Tracy Jean

    2015-01-01

    2.2 Cancer evolution . . . . . . . . . . . . . . . . . . . .5 Retrotransposition in cancer genomes 5.1 Retrotransposonrearrangements in cancer . . . . . . . . 2.3.2 Graph-based

  11. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    gas storage1.2 Compressed gas storage Technology is already well-of the compressed gas storage method is that it has been

  12. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    organic compounds and gas storage capabilities in nanoporousof materials for gas storage has been rather limited becausefree energy and gas storage and transport properties in

  13. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    organic frameworks for hydrogen storage Abstract Classicalcandidate: for DOE’s hydrogen storage target condition, it’shydrogen storage ..

  14. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    have great promise as hydrogen storage materials due tofor regenerative hydrogen storage applications. Addition ofrequired of a viable hydrogen storage system, but further

  15. Quantitative and high-throughput receptor affinity profiling system reveals distinct pathophysiological HIV-1 phenotypes

    E-Print Network [OSTI]

    Chikere, Kelechi C.

    2012-01-01

    in Journal of Virology: Palomares K, Pernet O, Vigant F,Immunity, In Press) Palomares K, Pernet O, Vigant F, Chikere

  16. High-throughput respirometric assay identifies predictive toxicophore of mitochondrial injury

    SciTech Connect (OSTI)

    Wills, Lauren P. [MitoHealth Inc., Charleston, SC 29403 (United States); Beeson, Gyda C.; Trager, Richard E.; Lindsey, Christopher C. [Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425 (United States); Beeson, Craig C. [MitoHealth Inc., Charleston, SC 29403 (United States); Peterson, Yuri K. [Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425 (United States); Schnellmann, Rick G., E-mail: schnell@musc.edu [Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425 (United States); Ralph H. Johnson VA Medical Center, Charleston, SC 29401 (United States)

    2013-10-15

    Many environmental chemicals and drugs negatively affect human health through deleterious effects on mitochondrial function. Currently there is no chemical library of mitochondrial toxicants, and no reliable methods for predicting mitochondrial toxicity. We hypothesized that discrete toxicophores defined by distinct chemical entities can identify previously unidentified mitochondrial toxicants. We used a respirometric assay to screen 1760 compounds (5 ?M) from the LOPAC and ChemBridge DIVERSet libraries. Thirty-one of the assayed compounds decreased uncoupled respiration, a stress test for mitochondrial dysfunction, prior to a decrease in cell viability and reduced the oxygen consumption rate in isolated mitochondria. The mitochondrial toxicants were grouped by chemical similarity and two clusters containing four compounds each were identified. Cheminformatic analysis of one of the clusters identified previously uncharacterized mitochondrial toxicants from the ChemBridge DIVERSet. This approach will enable the identification of mitochondrial toxicants and advance the prediction of mitochondrial toxicity for both drug discovery and risk assessment. - Highlights: • Respirometric assay conducted in RPTC to create mitochondrial toxicant database. • Chemically similar mitochondrial toxicants aligned as mitochondrial toxicophores • Mitochondrial toxicophore identifies five novel mitochondrial toxicants.

  17. Supplementary Materials: The AFLOW Standard for High-Throughput Materials Science Calculations

    E-Print Network [OSTI]

    Curtarolo, Stefano

    , [Cu4ACHTUNGTRENNUNG(Me3CCOO)8ACHTUNGTRENNUNG(teia)]·7.5PrOH (SNU-80a), were pre- pared by the reaction-crystal X-ray diffraction study of SNU-80a reveals that each teia coordinates four Cu2+ ions of four [Cu2 by PLATON.[11] The theoretical surface area of the framework of SNU-80a is 1440 m2 gŔ1 as estimated

  18. Encoded hydrogel microparticles for high-throughput molecular diagnostics and personalized medicine

    E-Print Network [OSTI]

    Chapin, Stephen Clifford

    2012-01-01

    The ability to accurately detect and quantify biological molecules in complex mixtures is crucial in basic research as well as in clinical settings. Advancements in genetic analysis, molecular diagnostics, and patient-tailored ...

  19. Examination of mammalian microRNAs by high-throughput sequencing

    E-Print Network [OSTI]

    Chiang, HyoJin Rosaria

    2011-01-01

    Small non-coding RNAs play an important role in a wide range of cellular events. MicroRNAs (miRNAs) are an abundant class of small RNAs that post-transcriptionally repress expression of their target genes. Since miRNA ...

  20. Antibody-functionalized nanoporous surfaces enable high throughput specific cell capture

    E-Print Network [OSTI]

    Mittal, Sukant

    2012-01-01

    Adhesion-based cell capture on surfaces in microfluidic devices forms the basis of numerous biomedical diagnostics and in vitro assays. Solid surface microfluidic platforms have been widely explored for biomedical diagnostics ...

  1. Polymers with hydro-responsive topography identified using high throughput AFM of an acrylate microarray

    E-Print Network [OSTI]

    Hook, Andrew L.

    Atomic force microscopy has been applied to an acrylate polymer microarray to achieve a full topographic characterisation. This process discovered a small number of hydro-responsive materials created from monomers with ...

  2. Development and implementation of high-throughput SNP genotyping in barley

    E-Print Network [OSTI]

    2009-01-01

    USA, 6 Department of Crop and Soil Science, Oregon State9 Department of Crop and Soil Sciences, Washington StateSciences, University of California (UCR), Riverside, CA, 92521, USA, 3 Scottish Crop

  3. A high-throughput digital imaging screen for the discovery and directed evolution of oxygenases

    E-Print Network [OSTI]

    Arnold, Frances H.

    numbers of crude enzyme samples. Results: Horseradish peroxidase (HRP) couples the phenolic products in degradation of xenobiotics) nearly always function as large, multimeric protein complexes that are only

  4. Density Functional Theory for High-Throughput Screening of Chemicals and Materials

    E-Print Network [OSTI]

    Fu, Jia

    2015-01-01

    Celzard, A. ; Fierro, V. Energy & Fuels 2005, 19, 573. (140)Snurr, R. Q. ; Hupp, J. T. Energy & Environmental ScienceH. -K. ; Zhou, H. -C. Energy & Environmental Science 2013,

  5. Characterization and High Throughput Analysis of Metal Hydrides for Hydrogen Storage

    E-Print Network [OSTI]

    Barcelo, Steven James

    2009-01-01

    palladium (Pd) to protect them from oxidation as well as to enhance hydrogenpalladium or nickel. These materials also offer an aid studies by catalyzing hydrogen

  6. A high-throughput behavioral paradigm for Drosophila olfaction -The Flywalk

    E-Print Network [OSTI]

    -delivery system Flywalk. In fifteen aligned small wind tunnels individual flies are exposed to repeated odor

  7. High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform

    E-Print Network [OSTI]

    Abalde-Cela, Sara; Gould, Anna; Liu, Xin; Kazamia, Elena; Smith, Alison G.; Abell, Chris

    2015-03-17

    . Both negative controls show essen- lation of cells. Those containing BG11 7. Azevedo AM, Prazeres DMF, Cabral JMS, Fonseca LP. 2005 Ethanol biosensors based on alcohol oxidase. Biosens. Bioelectron. 21, 235–247. (doi:10.1016/j. bios.2004.09.030) 8...

  8. High Throughput Isolation and Glycosylation Analysis of IgGVariability and Heritability of

    E-Print Network [OSTI]

    ., Glycobiology Division, Planinska 1, 10000 Zagreb, Croatia; §BIA Separations Ltd., Teslova 30, 1000 Ljubljana School of Medicine, S oltanska 2, Split, Croatia; **University of Za- greb, Faculty of Pharmacy and Biochemistry, Ante Kovac ic´ a 1, 10000 Zagreb, Croatia; Oxford Glycobiology Institute, Department of Bio

  9. High throughput low power decoder architectures for low density parity check codes 

    E-Print Network [OSTI]

    Selvarathinam, Anand Manivannan

    2005-11-01

    ?????????????????..?... 14 3 Iteration Logic Stage???????????????????..?... 15 4 Fully Parallel Architecture?????????????????..?.. 17 5 BER/FER Simulation Result for Different Values of M?????.??. 29 6 Iteration Stage ? Combinational Logic Scaling... Patterns?????? 46 11 H Matrix for M = N/12 and 10, 12 Patterns????????????.. 46 12 BER Performance for M = N/6 and Different Patterns????????. 49 13 FER Performance for M = N/6 and Different Patterns????????. 49 14 BER Performance for M = N/12...

  10. High-Throughput Screening Platform for Engineered Nanoparticle-Mediated Genotoxicity Using CometChip Technology

    E-Print Network [OSTI]

    Watson, Christa

    The likelihood of intentional and unintentional engineered nanoparticle (ENP) exposure has dramatically increased due to the use of nanoenabled products. Indeed, ENPs have been incorporated in many useful products and have ...

  11. Agenda from the U.S. Department of Energy's High Throughput Screening of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based Fuels Research at NREL AdvancedEnergyAdvocateRegisterHydrogen Storage

  12. Attendees list from the U.S. Department of Energy's High Throughput

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based Fuels Research atDepartment ofAtlanta

  13. Biochemical & Thermochemical High Throughput Characterization of Feedstocks for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum Based Fuels Researchof Energy|

  14. Algorithms and tools for high-throughput geometry-based analysis of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory of raregovAboutRecovery Act RecoveryTechnologiesAffiliatescrystalline porous

  15. Algorithms and tools for high-throughput geometry-based analysis of

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield MunicipalTechnicalInformation4563AbuseConnect Technical Report: Aging Studiescrystalline porous

  16. Improved Algae-based Biorefining and High-throughput Screening of Algal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLAN FOR THE SITE-218 58 84 168 167 164 1982-2016ARM-SGP

  17. Improving network performance on multicore systems: Impact of core affinities on high throughput flows

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenry Bellamy,Impact AssessmentsImproving Reactor

  18. Accounting for Memory Use, Cost, Throughput, and Latency in the Design of a Media Server

    E-Print Network [OSTI]

    Chang, Edward Y.

    : the per­stream costs and the worst­case initial latency before a new media request can be satisfied. WeAccounting for Memory Use, Cost, Throughput, and Latency in the Design of a Media Server Edward scheduling and data place­ ment ones. We show that, some disk latency reduction techniques can

  19. Distributed Throughput Optimization for ZigBee Cluster-Tree Networks

    E-Print Network [OSTI]

    Zhuang, Weihua

    1 Distributed Throughput Optimization for ZigBee Cluster-Tree Networks Yu-Kai Huang§, Ai-Chun Pang connectivity in inexpensive, portable, and mobile devices. Among the well-known ZigBee topologies, ZigBee will be generated. However, the restricted routing of a ZigBee cluster- tree network may not be able to provide

  20. SCHEDULING ABOVE MAC TO MAXIMIZE BATTERY LIFETIME AND THROUGHPUT IN WLANS

    E-Print Network [OSTI]

    Simunic, Tajana

    SCHEDULING ABOVE MAC TO MAXIMIZE BATTERY LIFETIME AND THROUGHPUT IN WLANS Edoardo Regini, Daeseob Diego La Jolla, CA 92093 {eregini, dalim, tajana}@ucsd.edu ABSTRACT Maximizing battery lifetime. This is because in heavy traffic conditions, the chance of nodes to successfully transmit a packet decreases