National Library of Energy BETA

Sample records for heterotrophic algae cxs

  1. algae

    National Nuclear Security Administration (NNSA)

    promote clean transportation fuels, that path could help bring the promise of algal biofuels closer to reality. As one of the fastest growing organisms on the planet, algae are...

  2. Algae to Biofuels

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algae to Biofuels Algae to Biofuels What if you could power your life using pond scum? Algae, plant-like aquatic microorganisms, produce oil similar to petroleum and can be grown almost anywhere, don't need to be fed and actually remove pollution from the air. algae Squeezing Power from Pond Scum Near industrial plants on undesirable land, scientists raise algae that suck up harmful exhaust and thrive in the non-drinkable wastewater. algae Why Algae? Algae produce at least 32 times more oil than

  3. Algae Biofuels Technology | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae Biofuels Technology Algae Biofuels Technology Algae Biofuels Technology PDF icon Algae Biofuels Technology More Documents & Publications The Promise and Challenge of Algae as...

  4. Magnetic separation of algae

    DOE Patents [OSTI]

    Nath, Pulak; Twary, Scott N.

    2016-04-26

    Described herein are methods and systems for harvesting, collecting, separating and/or dewatering algae using iron based salts combined with a magnetic field gradient to separate algae from an aqueous solution.

  5. Algae Biomass Summit

    Broader source: Energy.gov [DOE]

    The 9th annual Algae Biomass Summit will be hosted at the Washington Marriot Wardman Park in Washington D.C., September 29 – October 2, 2015. The event will gather leaders in algae biomass from all sectors. U.S. Department of Energy Undersecretary Franklin Orr will give a keynote address at the conference, and Bioenergy Technologies Office (BETO) Director Jonathan, Algae Program Manager Alison Goss Eng, and the BETO Algae Team will be in attendance.

  6. International Algae Symposium

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy’s Bioenergy Technologies Office Advanced Algal Systems Technology Manager Daniel Fishman represented the Office at the International Algae Symposium in Tokyo, Japan. Hosted at the University of Tsukuba’s newly established Algae Biomass Bioenergy Development Research Center, the symposium was an opportunity for algae researchers, policy makers, and industry leaders across the globe to learn about each other’s work.

  7. Algae Biotecnologia | Open Energy Information

    Open Energy Info (EERE)

    Algae Biotecnologia Jump to: navigation, search Name: Algae Biotecnologia Place: Sao Paulo, Sao Paulo, Brazil Product: Brazil-based 2nd generation ethanol producer. References:...

  8. Algae Derived Biofuel

    SciTech Connect (OSTI)

    Jahan, Kauser

    2015-03-31

    One of the most promising fuel alternatives is algae biodiesel. Algae reproduce quickly, produce oils more efficiently than crop plants, and require relatively few nutrients for growth. These nutrients can potentially be derived from inexpensive waste sources such as flue gas and wastewater, providing a mutual benefit of helping to mitigate carbon dioxide waste. Algae can also be grown on land unsuitable for agricultural purposes, eliminating competition with food sources. This project focused on cultivating select algae species under various environmental conditions to optimize oil yield. Membrane studies were also conducted to transfer carbon di-oxide more efficiently. An LCA study was also conducted to investigate the energy intensive steps in algae cultivation.

  9. BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae Biofuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae Biofuel BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae Biofuel BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae Biofuel

  10. algae | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    Home algae Sandia algae raceway paves path from lab to real-world applications Sandia California held a ribbon cutting ceremony for the Algae Raceway Testing Facility last week. The new facility will help scientists advance laboratory research to real-world applications. In a twist of geometry, an oval can make a line. The new algae raceway testing facility at Sandia...

  11. Florida Algae | Open Energy Information

    Open Energy Info (EERE)

    Algae Jump to: navigation, search Name: Florida Algae LLC Website: www.floridaalgae.com Coordinates: 27.6648274, -81.5157535 Show Map Loading map... "minzoom":false,"mappingse...

  12. Potential for Biofuels from Algae (Presentation)

    SciTech Connect (OSTI)

    Pienkos, P. T.

    2007-11-15

    Presentation on the potential for biofuels from algae presented at the 2007 Algae Biomass Summit in San Francisco, CA.

  13. Whole Algae Hydrothermal Liquefaction | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Liquefaction Whole Algae Hydrothermal Liquefaction Process Design and Economics for Whole Algae Hydrothermal Liquefaction, a paper from Pacific Northwest National Laboratory. PDF icon pnnl_whole_algae_liquefaction.pdf More Documents & Publications Pathways for Algal Biofuels Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae Hydrothermal Liquefaction 2013 Peer Review Presentations-Algae

  14. Commercial Algae Management | Open Energy Information

    Open Energy Info (EERE)

    Algae Management Jump to: navigation, search Name: Commercial Algae Management Address: 320 Arbor Lane Place: Franklin, NC Zip: 28734 Year Founded: 2002 Phone Number: 828-634-7070...

  15. Whole Algae Hydrothermal Liquefaction Technology Pathway (Technical...

    Office of Scientific and Technical Information (OSTI)

    Technical Report: Whole Algae Hydrothermal Liquefaction Technology Pathway Citation Details In-Document Search Title: Whole Algae Hydrothermal Liquefaction Technology Pathway This ...

  16. Fuel From Algae: Scaling and Commercialization of Algae Harvesting Technologies

    SciTech Connect (OSTI)

    2010-01-15

    Broad Funding Opportunity Announcement Project: Led by CEO Ross Youngs, AVS has patented a cost-effective dewatering technology that separates micro-solids (algae) from water. Separating micro-solids from water traditionally requires a centrifuge, which uses significant energy to spin the water mass and force materials of different densities to separate from one another. In a comparative analysis, dewatering 1 ton of algae in a centrifuge costs around $3,400. AVSs Solid-Liquid Separation (SLS) system is less energy-intensive and less expensive, costing $1.92 to process 1 ton of algae. The SLS technology uses capillary dewatering with filter media to gently facilitate water separation, leaving behind dewatered algae which can then be used as a source for biofuels and bio-products. The biomimicry of the SLS technology emulates the way plants absorb and spread water to their capillaries.

  17. Transgenic algae engineered for higher performance

    DOE Patents [OSTI]

    Unkefer, Pat J; Anderson, Penelope S; Knight, Thomas J

    2014-10-21

    The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase.

  18. BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae: for a Cleaner and...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae: for a Cleaner and Greener Tomorrow BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae: for a Cleaner and Greener Tomorrow BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae: for a Cleaner ...

  19. Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Whole Algae Hydrothermal Liquefaction Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae Hydrothermal Liquefaction Whole algae hydrothermal liquefaction is one of ...

  20. Webinar: Genetically Modified Algae: A Risk-Benefit Assessment...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae: A Risk-Benefit Assessment Webinar: Genetically Modified Algae: A Risk-Benefit Assessment Genetically Modified (GM) Algae: A Risk-Benefit Assessment PDF icon...

  1. Formation of algae growth constitutive relations for improved algae modeling.

    SciTech Connect (OSTI)

    Gharagozloo, Patricia E.; Drewry, Jessica L.

    2013-01-01

    This SAND report summarizes research conducted as a part of a two year Laboratory Directed Research and Development (LDRD) project to improve our abilities to model algal cultivation. Algae-based biofuels have generated much excitement due to their potentially large oil yield from relatively small land use and without interfering with the food or water supply. Algae mitigate atmospheric CO2 through metabolism. Efficient production of algal biofuels could reduce dependence on foreign oil by providing a domestic renewable energy source. Important factors controlling algal productivity include temperature, nutrient concentrations, salinity, pH, and the light-to-biomass conversion rate. Computational models allow for inexpensive predictions of algae growth kinetics in these non-ideal conditions for various bioreactor sizes and geometries without the need for multiple expensive measurement setups. However, these models need to be calibrated for each algal strain. In this work, we conduct a parametric study of key marine algae strains and apply the findings to a computational model.

  2. The Algae Foundation Announces New DOE Funded Education Initiative to Enhance Algae Workforce Development

    Broader source: Energy.gov [DOE]

    The Algae Foundation, a non-profit organization committed to expanding the algae industry through research, education, and outreach, announced plans at the 2015 Algae Biomass Organization Summit to develop an innovative formal degree program. The Department of Energy funded initiative seeks to strengthen workforce capabilities for commercial-scale algae production by developing a degree in algal cultivation technologies.

  3. Stochastic Forecasting of Algae Blooms in Lakes

    SciTech Connect (OSTI)

    Wang, Peng; Tartakovsky, Daniel M.; Tartakovsky, Alexandre M.

    2013-01-15

    We consider the development of harmful algae blooms (HABs) in a lake with uncertain nutrients inflow. Two general frameworks, Fokker-Planck equation and the PDF methods, are developed to quantify the resultant concentration uncertainty of various algae groups, via deriving a deterministic equation of their joint probability density function (PDF). A computational example is examined to study the evolution of cyanobacteria (the blue-green algae) and the impacts of initial concentration and inflow-outflow ratio.

  4. Whole Algae Hydrothermal Liquefaction Technology Pathway | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    order for the hydrothermal liquefaction of microalgae to be competitive with petroleum-derived gasoline-, diesel-, and jet-range hydrocarbon blendstocks. Whole Algae Hydrothermal...

  5. BioProcess Algae | Open Energy Information

    Open Energy Info (EERE)

    search Name: BioProcess Algae Place: Shenandoah, Iowa Sector: Biomass Product: US-based joint venture created to commercialize advanced photobioreactor technologies for...

  6. BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae: for a Cleaner and Greener

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Tomorrow | Department of Energy Algae: for a Cleaner and Greener Tomorrow BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae: for a Cleaner and Greener Tomorrow BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae: for a Cleaner and Greener Tomorrow

  7. 2013 Peer Review Presentations-Algae | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae 2013 Peer Review Presentations-Algae The Bioenergy Technologies Office hosted its 2013 Project Peer Review on May 20-24, 2015, at the Hilton Mark Center in Alexandria, Virginia. The presentations from the algae session are available to view and download below. For detailed session descriptions and presentation titles, view the 2013 Project Peer Review Program Booklet. PDF icon algae_abodeely_9131.pdf PDF icon algae_anderson_9321.pdf PDF icon algae_bagwell_9615.pdf PDF icon

  8. Effect of Dead Algae on Soil Permeability

    SciTech Connect (OSTI)

    Harvey, R.S.

    2003-02-21

    Since existing basins support heavy growths of unicellular green algae which may be killed by temperature variation or by inadvertent pH changes in waste and then deposited on the basin floor, information on the effects of dead algae on soil permeability was needed. This study was designed to show the effects of successive algal kills on the permeability of laboratory soil columns.

  9. Hydrogen metabolism of photosynthetic bacteria and algae

    SciTech Connect (OSTI)

    Kumazawa, S.; Mitsui, A.

    1982-01-01

    The metabolism, metabolic pathways and biochemistry of hydrogen in photosynthetic bacteria and algae are reviewed. Detailed information on the occurrence and measurement of hydrogenase activity is presented. Hydrogen production rates for different species of algae and bacteria are presented. 173 references, 1 figure, 7 tables.

  10. Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient Recycling, and Wastewater Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient Recycling, and Wastewater ...

  11. Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling October 5, 2015 - 12:16pm Addthis Ryan Davis ...

  12. Nanotechnology and algae biofuels exhibits open July 26 at the...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nanotechnology and algae biofuels exhibits open July 26 Nanotechnology and algae biofuels exhibits open July 26 at the Bradbury Science Museum The Bradbury Science Museum is...

  13. Crow Nation Students Participate in Algae Biomass Research Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Crow Nation Students Participate in Algae Biomass Research Project Crow Nation Students Participate in Algae Biomass Research Project October 22, 2012 - 3:44pm Addthis Crow Nation...

  14. California: Breakthrough in Algae Biology | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakthrough in Algae Biology California: Breakthrough in Algae Biology January 31, 2014 - 12:00am Addthis Researchers at the Scripps Institution of Oceanography at University of ...

  15. The Arizona Center for Algae Technology and Innovation | Open...

    Open Energy Info (EERE)

    Arizona Center for Algae Technology and Innovation Jump to: navigation, search Name: The Arizona Center for Algae Technology and Innovation Abbreviation: AzCATI Address: 7418 East...

  16. Flocculation of model algae under shear.

    SciTech Connect (OSTI)

    Pierce, Flint; Lechman, Jeremy B.

    2010-11-01

    We present results of molecular dynamics simulations of the flocculation of model algae particles under shear. We study the evolution of the cluster size distribution as well as the steady-state distribution as a function of shear rates and algae interaction parameters. Algal interactions are modeled through a DLVO-type potential, a combination of a HS colloid potential (Everaers) and a yukawa/colloid electrostatic potential. The effect of hydrodynamic interactions on aggregation is explored. Cluster strucuture is determined from the algae-algae radial distribution function as well as the structure factor. DLVO parameters including size, salt concentration, surface potential, initial volume fraction, etc. are varied to model different species of algae under a variety of environmental conditions.

  17. Method and apparatus for processing algae

    DOE Patents [OSTI]

    Chew, Geoffrey; Reich, Alton J.; Dykes, Jr., H. Waite; Di Salvo, Roberto

    2012-07-03

    Methods and apparatus for processing algae are described in which a hydrophilic ionic liquid is used to lyse algae cells. The lysate separates into at least two layers including a lipid-containing hydrophobic layer and an ionic liquid-containing hydrophilic layer. A salt or salt solution may be used to remove water from the ionic liquid-containing layer before the ionic liquid is reused. The used salt may also be dried and/or concentrated and reused. The method can operate at relatively low lysis, processing, and recycling temperatures, which minimizes the environmental impact of algae processing while providing reusable biofuels and other useful products.

  18. EERE Success Story—California: Breakthrough in Algae Biology

    Broader source: Energy.gov [DOE]

    Breakthrough in algae biology will have a significant impact in the economics of algal biofuel production.

  19. 2011 Biomass Program Platform Peer Review: Algae

    SciTech Connect (OSTI)

    Yang, Joyce

    2012-02-01

    This document summarizes the recommendations and evaluations provided by an independent external panel of experts at the 2011 U.S. Department of Energy Biomass Program’s Algae Platform Review meeting.

  20. Algae Testbed Public-Private Partnership

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    atp3.org 1 John A. McGowen PhD, PMP Director of Operations and Program Management Arizona Center for Algae Technology and Innovation (AzCATI) Arizona State University July 30, ...

  1. Wastewater Reclamation and Biofuel Production Using Algae | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Wastewater Reclamation and Biofuel Production Using Algae Wastewater Reclamation and Biofuel Production Using Algae Breakout Session 2-A: The Future of Algae-Based Biofuels Wastewater Reclamation and Biofuel Production Using Algae Tryg Lundquist, Associate Professor, California Polytechnic State University, San Luis Obispo PDF icon lundquist_bioenergy_2015.pdf More Documents & Publications CX-009557: Categorical Exclusion Determination 2013 Peer Review Presentations-Algae ATP3

  2. Algae Testbed Public Private Partnership Workshop on Principles and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Processes: Algae Culture Management, Production and Downstream Harvesting | Department of Energy Algae Testbed Public Private Partnership Workshop on Principles and Processes: Algae Culture Management, Production and Downstream Harvesting Algae Testbed Public Private Partnership Workshop on Principles and Processes: Algae Culture Management, Production and Downstream Harvesting May 16, 2016 1:00PM CDT to May 20, 2016 11:00AM CDT Santa Fe Community College 6401 Richards Avenue Santa Fe, New

  3. Turning Algae into Energy in New Mexico

    ScienceCinema (OSTI)

    Sayre, Richard; Olivares, Jose; Lammers, Peter

    2014-06-24

    Los Alamos National Laboratory, as part of the New Mexico Consortium - comprised of New Mexico's major research universities, the Lab, and key industry partners - is conducting research into using algae as a feed stock for a renewable source of fuels, and other products. There are hundreds of thousands of different algae species on Earth. They account for approximately half of the net photosynthesis on the planet, yet they have not been used in any kind of a large scale by humanity, with just a few exceptions. And yet, the biomass is easy to transform into useful products, including fuels, and they contain many other natural products that have high value. In this video Los Alamos and New Mexico State University scientists outline the opportunities and challenges of using science to turn algae into energy.

  4. Turning Algae into Energy in New Mexico

    SciTech Connect (OSTI)

    Sayre, Richard; Olivares, Jose; Lammers, Peter

    2013-07-29

    Los Alamos National Laboratory, as part of the New Mexico Consortium - comprised of New Mexico's major research universities, the Lab, and key industry partners - is conducting research into using algae as a feed stock for a renewable source of fuels, and other products. There are hundreds of thousands of different algae species on Earth. They account for approximately half of the net photosynthesis on the planet, yet they have not been used in any kind of a large scale by humanity, with just a few exceptions. And yet, the biomass is easy to transform into useful products, including fuels, and they contain many other natural products that have high value. In this video Los Alamos and New Mexico State University scientists outline the opportunities and challenges of using science to turn algae into energy.

  5. Energy 101: Algae-to-Fuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    August 13, 2013 - 2:53pm Addthis Learn about algae, a fast-growing, renewable resource ... This edition of Energy 101 shares the benefits of an algae-fueled future. For more ...

  6. Reviving Algae from the (Almost) Dead - News Feature | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reviving Algae from the (Almost) Dead October 31, 2014 Photo of a man in a lab coat standing next to green algae bubbling in containers. NREL Research Technician Nick Sweeney...

  7. 2011 Biomass Program Platform Review Report: Algae | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Review Report: Algae 2011 Biomass Program Platform Review Report: Algae This document summarizes the recommendations and evaluations provided by an independent external panel of experts at the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Biomass Program's Algae Platform Review meeting, held on April 7-8, 2011, at the Doubletree Hotel in Annapolis, Maryland. PDF icon 2011_algae_review.pdf More Documents & Publications 2011 Biomass Program Platform Peer Review:

  8. Research Leads to Improved Fuel Yields from Smaller Antenna Algae

    Broader source: Energy.gov [DOE]

    A study funded by the Energy Department could lead to big improvements in alternative fuel production. Researchers at the University of California, Berkeley have discovered that if particular genes are missing in certain strains of algae, the algae can produce more hydrogen and other fuel from full sunlight than the ordinary algae.

  9. Phototrophic Biofilm Assembly in Microbial-Mat-Derived Unicyanobacterial Consortia: Model Systems for the Study of Autotroph-Heterotroph Interactions

    SciTech Connect (OSTI)

    Cole, Jessica K.; Hutchison, Janine R.; Renslow, Ryan S.; Kim, Young-Mo; Chrisler, William B.; Engelmann, Heather E.; Dohnalkova, Alice; Hu, Dehong; Metz, Thomas O.; Fredrickson, Jim K.; Lindemann, Stephen R.

    2014-04-07

    Though microbial autotroph-heterotroph interactions influence biogeochemical cycles on a global scale, the diversity and complexity of natural systems and their intractability to in situ environmental manipulation makes elucidation of the principles governing these interactions challenging. Examination of primary succession during phototrophic biofilm assembly provides a robust means by which to elucidate the dynamics of such interactions and determine their influence upon recruitment and maintenance of phylogenetic and functional diversity in microbial communities. We isolated and characterized two unicyanobacterial consortia from the Hot Lake phototrophic mat, quantifying the structural and community composition of their assembling biofilms. The same heterotrophs were retained in both consortia and included members of Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes, taxa frequently reported as consorts of microbial photoautotrophs. Cyanobacteria led biofilm assembly, eventually giving way to a late heterotrophic bloom. The consortial biofilms exhibited similar patterns of assembly, with the relative abundances of members of Bacteroidetes and Alphaproteobacteria increasing and members of Gammaproteobacteria decreasing as colonization progressed. Despite similar trends in assembly at higher taxa, the consortia exhibited substantial differences in community structure at the species level. These similar patterns of assembly with divergent community structures suggest that, while similar niches are created by the metabolism of the cyanobacteria, the resultant webs of autotroph-heterotroph and heterotroph-heterotroph interactions driving metabolic exchange are specific to each primary producer. Altogether, our data support these Hot Lake unicyanobacterial consortia as generalizable model systems whose simplicity and tractability permit the deciphering of community assembly principles relevant to natural microbial communities.

  10. Reviving Algae from the (Almost) Dead - News Feature | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reviving Algae from the (Almost) Dead October 31, 2014 Photo of a man in a lab coat standing next to green algae bubbling in containers. NREL Research Technician Nick Sweeney inoculates algae being grown in a tent reactor. Algae brought back to active states from cryogenic tanks need aerobic environments to meet their full potential as biofuels. Photo by Dennis Schroeder, NREL Tucked away in darkness and almost dead, algae can emerge from a frigid and foggy environment to live again-and perhaps

  11. Algae Raceway to speed path to biofuels

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algae Raceway to speed path to biofuels - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management

  12. BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae Biofuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae Biofuel BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae Biofuel BIOENERGIZEME INFOGRAPHIC CHALLENGE: Algae Biofuel This infographic was created by students from Seward HS in Seward, AK, as part of the U.S. Department of Energy-BioenergizeME Infographic Challenge. The BioenergizeME Infographic Challenge encourages young people to improve their foundational understanding of bioenergy, which is a broad and complex topic. The ideas expressed in these infographics reflect where students are in the

  13. NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Production - News Releases | NREL Scientists Find Key Function for Ferredoxins in Algae Hydrogen Production Two of six iron-rich proteins shown to have role in algae metabolism; discovery could lead to enhanced hydrogen production February 10, 2014 Scientists at the Energy Department's National Renewable Energy Laboratory have demonstrated that just two of six iron-sulfur-containing ferredoxins in a representative species of algae promote electron transfers to and from hydrogenases. The

  14. Algae to Bio-Crude in Less Than 60 Minutes

    SciTech Connect (OSTI)

    Elliott, Doug

    2013-12-17

    Engineers have created a chemical process that produces useful crude oil just minutes after engineers pour in harvested algae -- a verdant green paste with the consistency of pea soup. The PNNL team combined several chemical steps into one continuous process that starts with an algae slurry that contains as much as 80 to 90 percent water. Most current processes require the algae to be dried -- an expensive process that takes a lot of energy. The research has been licensed by Genifuel Corp.

  15. Method for producing hydrogen and oxygen by use of algae

    DOE Patents [OSTI]

    Greenbaum, Elias

    1984-01-01

    Efficiency of process for producing H.sub.2 by subjecting algae in an aqueous phase to light irradiation is increased by culturing algae which has been bleached during a first period of irradiation in a culture medium in an aerobic atmosphere until it has regained color and then subjecting this algae to a second period of irradiation wherein hydrogen is produced at an enhanced rate.

  16. Real Time Diagnostics for Algae-final-sm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Real-time Monitoring And Diagnostics Detecting pathogens and predators to quickly recover from pond crashes Algal Pond Crash Detection Sandia National Laboratories is developing a suite of complementary technologies to help the emerging algae industry detect and quickly recover from algal pond crashes, an obstacle to large-scale algae cultivation for biofuels. Because of the way algae is grown and produced in most algal ponds, they are prone to attack by fungi, rotifers, viruses or other

  17. Method for producing hydrogen and oxygen by use of algae

    DOE Patents [OSTI]

    Greenbaum, E.

    1982-06-16

    Efficiency of process for producing H/sub 2/ by subjecting algae in an aqueous phase to light irradiation is increased by culturing algae which has been bleached during a first period of irradiation in a culture medium in an aerobic atmosphere until it has regained color and then subjecting this algae to a second period of irradiation wherein hydrogen is produced at an enhanced rate.

  18. Method and apparatus for iterative lysis and extraction of algae

    DOE Patents [OSTI]

    Chew, Geoffrey; Boggs, Tabitha; Dykes, Jr., H. Waite H.; Doherty, Stephen J.

    2015-12-01

    A method and system for processing algae involves the use of an ionic liquid-containing clarified cell lysate to lyse algae cells. The resulting crude cell lysate may be clarified and subsequently used to lyse algae cells. The process may be repeated a number of times before a clarified lysate is separated into lipid and aqueous phases for further processing and/or purification of desired products.

  19. Algae to Bio-Crude in Less Than 60 Minutes

    ScienceCinema (OSTI)

    Elliott, Doug

    2014-06-02

    Engineers have created a chemical process that produces useful crude oil just minutes after engineers pour in harvested algae -- a verdant green paste with the consistency of pea soup. The PNNL team combined several chemical steps into one continuous process that starts with an algae slurry that contains as much as 80 to 90 percent water. Most current processes require the algae to be dried -- an expensive process that takes a lot of energy. The research has been licensed by Genifuel Corp.

  20. Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient Recycling,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Wastewater | Department of Energy Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient Recycling, and Wastewater Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient Recycling, and Wastewater Breakout Session 2-C: Biogas and Beyond: Challenges and Opportunities for Advanced Biofuels from Wet-Waste Feedstocks Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient Recycling, and Wastewater Jordi Perez, Scientist, SRI International PDF icon

  1. Overall Energy Considerations for Algae Species Comparison and Selection in Algae-to-Fuels Processes

    SciTech Connect (OSTI)

    Link, D.; Kail, B.; Curtis, W.; Tuerk,A.

    2011-01-01

    The controlled growth of microalgae as a feedstock for alternative transportation fuel continues to receive much attention. Microalgae have the characteristics of rapid growth rate, high oil (lipid) content, and ability to be grown in unconventional scenarios. Algae have also been touted as beneficial for CO{sub 2} reuse, as algae can be grown using CO{sub 2} emissions from fossil-based energy generation. Moreover, algae does not compete in the food chain, lessening the 'food versus fuel' debate. Most often, it is assumed that either rapid production rate or high oii content should be the primary factor in algae selection for algae-to-fuels production systems. However, many important characteristics of algae growth and lipid production must be considered for species selection, growth condition, and scale-up. Under light limited, high density, photoautotrophic conditions, the inherent growth rate of an organism does not affect biomass productivity, carbon fixation rate, and energy fixation rate. However, the oil productivity is organism dependent, due to physiological differences in how the organisms allocate captured photons for growth and oil production and due to the differing conditions under which organisms accumulate oils. Therefore, many different factors must be considered when assessing the overall energy efficiency of fuel production for a given algae species. Two species, Chlorella vulgaris and Botryococcus braunii, are popular choices when discussing algae-to-fuels systems. Chlorella is a very robust species, often outcompeting other species in mixed-culture systems, and produces a lipid that is composed primarily of free fatty acids and glycerides. Botryococcus is regarded as a slower growing species, and the lipid that it produces is characterized by high hydrocarbon content, primarily C28-C34 botryococcenes. The difference in growth rates is often considered to be an advantage oiChlorella. However, the total energy captured by each algal species in the same photobioreactor system should be similar at light limited growth conditions based on photon flux. It is how the algae 'allocate' this energy captured that will vary: Data will be presented that shows that Botryococcus invests greater energy in oil production than Chlorella under these growth conditions. In essence, the Chlorella can grow 'fast and lean' or can be slowed to grow 'slow and fat'. The overall energy potential between the Chlorella and Botryococcus, then, becomes much more equivalent on a per-photon basis. This work will indicate an interesting relationship between two very different algae species, in terms of growth rate, lipid content and composition, and energy efficiency of the overall process. The presentation will indicate that in light-limited growth, it cannot be assumed that either rapid growth rate or lipid production rate can be used as stand-alone indicators of which species-lipid relationships will truly be more effective in algae-to-fuels scenarios.

  2. Energy 101: Algae-to-Fuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Algae-to-Fuel Energy 101: Algae-to-Fuel Addthis Description As America takes steps to improve our energy security, home-grown fuel sources are more important that ever. One of the fuel sources of the future is algae, small aquatic organisms that convert sunlight into energy and store it in the form of oil. Scientists and engineers at the Energy Department and its national laboratories are researching the best strains of algae and developing the most efficient farming practices. This video shows

  3. Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling

    Broader source: Energy.gov [DOE]

    Algae are a promising source of renewable biofuels and bioproducts, and researchers at Sandia National Laboratories in Livermore, California, are taking a step toward realizing the promise of...

  4. Method and apparatus for lysing and processing algae

    DOE Patents [OSTI]

    Chew, Geoffrey; Reich, Alton J.; Dykes, Jr., H. Waite H.; Di Salvo, Roberto

    2013-03-05

    Methods and apparatus for processing algae are described in which a hydrophilic ionic liquid is used to lyse algae cells at lower temperatures than existing algae processing methods. A salt or salt solution is used as a separation agent and to remove water from the ionic liquid, allowing the ionic liquid to be reused. The used salt may be dried or concentrated and reused. The relatively low lysis temperatures and recycling of the ionic liquid and salt reduce the environmental impact of the algae processing while providing biofuels and other useful products.

  5. Pilot-Scale MixotrophicAlgae Integrated Biorefinery(IBR)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    short- and long-term job creation for construction, operators, biologists, chemists, ... Hydroprocessing 1 - PROJECT OVERVIEW * History: BioProcess Algae was formed in 2008 to ...

  6. Sandia Energy - Better Monitoring and Diagnostics Tackle Algae...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algae Biofuel Pond Crash Problem Home Renewable Energy Energy Transportation Energy Biofuels Capabilities News News & Events Research & Capabilities Systems Analysis Biomass...

  7. Energy 101 | Algae-to-Fuel | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    But that's exactly what Energy Department scientists and researchers are exploring right now - strategies to produce clean, renewable biofuel from algae. In this edition of our ...

  8. Algae Raceway Testing Facility Brings Algal Biofuels One Step...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Advanced Algal Systems Program Manager, Alison Goss Eng, and Technology Manager, Daniel ... Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling Ryan Davis and Sandia ...

  9. Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Microalgal biomass grown via autotrophic pathways is dewatered to 20% solids concentration. * The slurry of whole algae reacts in a pressure vessel (2000-3000 pounds per square ...

  10. Algae Biofuels Co-Location Assessment Tool

    Energy Science and Technology Software Center (OSTI)

    2013-09-18

    ABCLAT was built to help any model user with spatially explicit Nitrogen, Phosphorous, and Carbon Dioxide nutrient flux information, and solar resource information evaluate algal cultivation potential. Initial applications of this modeling framework include Algae Biofuels Co-Location Assessment Tool Canada and Australia. The Canadian application was copyrighted November 29th 2011 as the Algae Biofuels Co-Location Assessment Tool for Canada. This copyright assertion is for the general framework from which any country or region with themore » requisite data could create a regionally specific application. The ABCLAT model framework developed by SNL looks at the growth potential in a given region as a function of available nutrients from wastewater and other sources, carbon dioxide from power plants, available solar potential, and if available, land cover and use information. The model framework evaluates the biomass potential, fixed carbon dioxide, potential algal biocrude and required land area for nutrient sources. ABCLAT is built with an object-oriented software program that can provide an easy to use interface for exploring questions related to aigal biomass production.« less

  11. UTEX The Culture Collection of Algae at The University of Texas...

    Open Energy Info (EERE)

    UTEX The Culture Collection of Algae at The University of Texas at Austin Jump to: navigation, search Name: University of Texas at Austin The Culture Collection of Algae...

  12. CX-005693: Categorical Exclusion Determination | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    693: Categorical Exclusion Determination CX-005693: Categorical Exclusion Determination Solazyme Integrated Biorefinery (SzIBR): Diesel Fuels from Heterotrophic Algae CX(s) Applied: A9, B3.6 Date: 04/12/2011 Location(s): Peoria, Illinois Office(s): Energy Efficiency and Renewable Energy, Golden Field Office Department of Energy (DOE) is proposing to provide federal funding to Solazyme to build, operate and optimize a pilot-scale Solazyme Integrated Biorefinery (SzIBR). DOE completed the National

  13. Nitrogen control of 13C enrichment in heterotrophic organs relative to leaves in a landscape-building desert plant species

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhang, J.; Gu, L.; Bao, F.; Cao, Y.; Hao, Y.; He, J.; Li, J.; Li, Y.; Ren, Y.; Wang, F.; et al

    2014-09-10

    A longstanding puzzle in isotope studies of C3 plant species is that heterotrophic plant organs (e.g., stems, roots, seeds, and fruits) tend to be enriched in 13C compared to the autotrophic organ (leaves) that provides them with photosynthate. Our inability to explain this puzzle suggests key deficiencies in understanding post-photosynthetic metabolic processes. It also limits the effectiveness of applications of stable carbon isotope analyses in a variety of scientific disciplines ranging from plant physiology to global carbon cycle studies. To gain insight into this puzzle, we excavated whole plant architectures of Nitraria tangutorum Bobrov, a C3 species that has anmore » exceptional capability of fixing sands and building sand dunes, in two deserts in northwestern China. We systematically and simultaneously measured carbon isotope ratios and nitrogen and phosphorous contents of different parts of the excavated plants. We also determined the seasonal variations in leaf carbon isotope ratios on nearby intact plants of N. tangutorum. We found, for the first time, that higher nitrogen contents in heterotrophic organs were significantly correlated with increased heterotrophic 13C enrichment compared to leaves. However, phosphorous contents had no effect on the enrichment. In addition, new leaves had carbon isotope ratios similar to roots but were progressively depleted in 13C as they matured. We concluded that a nitrogen-mediated process, probably the refixation of respiratory CO2 by phosphoenolpyruvate (PEP) carboxylase, was responsible for the differences in 13C enrichment among different heterotrophic organs while processes within leaves or during phloem loading may contribute to the overall autotrophic – heterotrophic difference in carbon isotope compositions.« less

  14. Nitrogen control of 13C enrichment in heterotrophic organs relative to leaves in a landscape-building desert plant species

    SciTech Connect (OSTI)

    Zhang, Jinxin [Chinese Academy of Forestry; Gu, Lianhong [ORNL

    2014-01-01

    A longstanding puzzle in isotopic studies of C3 plant species is that heterotrophic plant organs (e.g., stems, roots, seeds, and fruits) tend to be enriched in 13C compared to the autotrophic organ (leaves) that provides them with photosynthate. Our inability to explain this puzzle suggests key deficiencies in understanding post-photosynthetic metabolic processes. It also limits the effectiveness of applications of stable carbon isotope analyses in a variety of scientific disciplines ranging from plant physiology to global carbon cycle studies. To gain insight into this puzzle, we excavated whole plant architectures of Nitraria tangutorum Bobrov, a C3 species that has an exceptional capability of fixing sands and building sand dunes, in two deserts in northwestern China. We systematically and simultaneously measured carbon isotopic ratios and nitrogen and phosphorous concentrations of different parts of the excavated plants. We also determined the seasonal variations in leaf carbon isotopic ratios on nearby intact plants of N. tangutorum. We found that higher nitrogen concentrations in heterotrophic organs were significantly correlated with increased heterotrophic 13C enrichment compared to leaves. However, phosphorous concentrations had no effect on the enrichment. In addition, new leaves had carbon isotopic ratios similar to roots but were progressively depleted in 13C as they matured. We concluded that a nitrogen-mediated process, probably the refixation of respiratory CO2 by phosphoenolpyruvate (PEP) carboxylase, was responsible for the differences in 13C enrichment among different heterotrophic organs while processes within leaves or during phloem loading may contribute to the overall autotrophic heterotrophic difference in carbon isotopic compositions.

  15. Isoprenoid biosynthesis in eukaryotic phototrophs: A spotlight on algae

    SciTech Connect (OSTI)

    Lohr M.; Schwender J.; Polle, J. E. W.

    2012-04-01

    Isoprenoids are one of the largest groups of natural compounds and have a variety of important functions in the primary metabolism of land plants and algae. In recent years, our understanding of the numerous facets of isoprenoid metabolism in land plants has been rapidly increasing, while knowledge on the metabolic network of isoprenoids in algae still lags behind. Here, current views on the biochemistry and genetics of the core isoprenoid metabolism in land plants and in the major algal phyla are compared and some of the most pressing open questions are highlighted. Based on the different evolutionary histories of the various groups of eukaryotic phototrophs, we discuss the distribution and regulation of the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways in land plants and algae and the potential consequences of the loss of the MVA pathway in groups such as the green algae. For the prenyltransferases, serving as gatekeepers to the various branches of terpenoid biosynthesis in land plants and algae, we explore the minimal inventory necessary for the formation of primary isoprenoids and present a preliminary analysis of their occurrence and phylogeny in algae with primary and secondary plastids. The review concludes with some perspectives on genetic engineering of the isoprenoid metabolism in algae.

  16. Solazyme Developing Cheaper Algae Biofuels, Brings Jobs to Pennsylvania |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Solazyme Developing Cheaper Algae Biofuels, Brings Jobs to Pennsylvania Solazyme Developing Cheaper Algae Biofuels, Brings Jobs to Pennsylvania August 6, 2010 - 2:00pm Addthis A $20 million Recovery Act award will help Solazyme take production from tens of thousands of gallons a year of its algae "drop-in" oil to an annual production capacity of over half a million gallons. | Photo courtesy of Solazyme, Inc. | A $20 million Recovery Act award will help Solazyme

  17. Photobiological hydrogen production with switchable photosystem-II designer algae

    DOE Patents [OSTI]

    Lee, James Weifu

    2014-02-18

    A process for enhanced photobiological H.sub.2 production using transgenic alga. The process includes inducing exogenous genes in a transgenic alga by manipulating selected environmental factors. In one embodiment inducing production of an exogenous gene uncouples H.sub.2 production from existing mechanisms that would downregulate H.sub.2 production in the absence of the exogenous gene. In other embodiments inducing an exogenous gene triggers a cascade of metabolic changes that increase H.sub.2 production. In some embodiments the transgenic alga are rendered non-regenerative by inducing exogenous transgenes for proton channel polypeptides that are targeted to specific algal membranes.

  18. Unique Bioreactor Finds Algae's Sweet Spot - News Feature | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Bioreactor Finds Algae's Sweet Spot February 18, 2014 Close-up photo of a vial of green algae. Enlarge image Aeration helps algae grow and helps replicate real-life conditions in the Simulated Algal Growth Environment (SAGE) reactor at NREL. The reactor controls light and temperature, helping scientists determine not just what strain will grow the best, but where in the United States it may do so. Ideal strains can be harvested for their lipids, proteins, and sugars for use in biofuels.

  19. Evaluation of defatted and whole algae as feed ingredients for the marine shrimp, litopenaeus vannamei

    SciTech Connect (OSTI)

    Morgan, J. L.; Patnaik, S.; Gatlin, III, D. M.; Lawrence, A. L.

    2012-06-13

    Evaluation of defatted and whole algae as feed ingredients for the marine shrimp, litopenaeus vannamei

  20. Crow Nation Students Participate in Algae Biomass Research Project

    Broader source: Energy.gov [DOE]

    Student interns from the Crow Tribe in Montana participate in an algae biomass research project that could help prepare them for cleantech jobs and pave the way for their Tribe to produce clean, renewable energy.

  1. Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling

    Broader source: Energy.gov [DOE]

    Algae are a promising source of renewable biofuels and bioproducts, and researchers at Sandia National Laboratories in Livermore, California, are taking a step toward realizing the promise of sustainable, cost-effective algal biofuels for the American public.

  2. ATP3 Algae Testbed Public-Private Partnership

    Broader source: Energy.gov [DOE]

    Breakout Session 3B—Integration of Supply Chains III: Algal Biofuels Strategy ATP3 Algae Testbed Public-Private Partnership John A. McGowen, Director of Operations and Program, Arizona State University, AzCATI and ATP3

  3. Whole Turf Algae to biofuels-final-sm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | SciTech Connect Technical Report: Whole Algae Hydrothermal Liquefaction Technology Pathway Citation Details In-Document Search Title: Whole Algae Hydrothermal Liquefaction Technology Pathway This technology pathway case investigates the feasibility of using whole wet microalgae as a feedstock for conversion via hydrothermal liquefaction. Technical barriers and key research needs have been assessed in order for the hydrothermal liquefaction of microalgae to be competitive with

  4. Whole Algae Hydrothermal Liquefaction Technology Pathway | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Liquefaction Technology Pathway Whole Algae Hydrothermal Liquefaction Technology Pathway This technology pathway case investigates the feasibility of using whole wet microalgae as a feedstock for conversion via hydrothermal liquefaction. Technical barriers and key research needs have been assessed in order for the hydrothermal liquefaction of microalgae to be competitive with petroleum-derived gasoline-, diesel-, and jet-range hydrocarbon blendstocks. PDF icon Whole Algae Hydrothermal

  5. BETO Deputy Director Publishes Commentary on Development of Algae as

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Renewable Energy Source | Department of Energy Deputy Director Publishes Commentary on Development of Algae as Renewable Energy Source BETO Deputy Director Publishes Commentary on Development of Algae as Renewable Energy Source March 30, 2015 - 1:28pm Addthis Bioenergy Technologies Office (BETO) Deputy Director Dr. Valerie Sarisky-Reed's commentary, "Algal Progress Report," was published in the February edition of the bimonthly research journal Industrial Biotechnology. Her

  6. Algae culture for cattle feed and water purification. Final report

    SciTech Connect (OSTI)

    Varani, F.T.; Schellenbach, S.; Veatch, M.; Grover, P.; Benemann, J.

    1980-05-16

    The feasibility of algae growth on centrate from anaerobic digester effluent and the refeed of both effluent solids and the algae to feedlot cattle were investigated. The digester was operated with dirt feedlot manure. The study serves as a supplement for the work to design a utility sized digester for the City of Lamar to convert local feedlot manure into a fuel gas. The biogas produced would power the electrical generation plant already in service. Previous studies have established techniques of digester operation and the nutritional value for effluent solids as fed to cattle. The inclusion of a single-strain of algae, Chlorella pyrenidosa in the process was evaluated here for its capability (1) to be grown in both open and closed ponds of the discharge water from the solids separation part of the process, (2) to purify the discharge water, and (3) to act as a growth stimulant for cattle feed consumption and conversion when fed at a rate of 6 grams per head per day. Although it was found that the algae could be cultured and grown on the discharge water in the laboratory, the study was unable to show that algae could accomplish the other objectives successfully. However, the study yielded supplementary information useful to the overall process design of the utility plant. This was (1) measurement of undried digester solids fed to cattle in a silage finishing ration (without algae) at an economic value of $74.99 per dry ton based on nutritional qualities, (2) development of a centrate treatment system to decolorize and disinfect centrate to allow optimum algae growth, and (3) information on ionic and mass balances for the digestion system. It is the recommendation of this study that algae not be used in the process in the Lamar bioconversion plant.

  7. Whole Algae Hydrothermal Liquefaction Technology Pathway (Technical Report)

    Office of Scientific and Technical Information (OSTI)

    | SciTech Connect Whole Algae Hydrothermal Liquefaction Technology Pathway Citation Details In-Document Search Title: Whole Algae Hydrothermal Liquefaction Technology Pathway This technology pathway case investigates the feasibility of using whole wet microalgae as a feedstock for conversion via hydrothermal liquefaction. Technical barriers and key research needs have been assessed in order for the hydrothermal liquefaction of microalgae to be competitive with petroleum-derived gasoline-,

  8. Development of Green Fuels From Algae - The University of Tulsa

    SciTech Connect (OSTI)

    Crunkleton, Daniel; Price, Geoffrey; Johannes, Tyler; Cremaschi, Selen

    2012-12-03

    The general public has become increasingly aware of the pitfalls encountered with the continued reliance on fossil fuels in the industrialized world. In response, the scientific community is in the process of developing non-fossil fuel technologies that can supply adequate energy while also being environmentally friendly. In this project, we concentrate on “green fuels” which we define as those capable of being produced from renewable and sustainable resources in a way that is compatible with the current transportation fuel infrastructure. One route to green fuels that has received relatively little attention begins with algae as a feedstock. Algae are a diverse group of aquatic, photosynthetic organisms, generally categorized as either macroalgae (i.e. seaweed) or microalgae. Microalgae constitute a spectacularly diverse group of prokaryotic and eukaryotic unicellular organisms and account for approximately 50% of global organic carbon fixation. The PI’s have subdivided the proposed research program into three main research areas, all of which are essential to the development of commercially viable algae fuels compatible with current energy infrastructure. In the fuel development focus, catalytic cracking reactions of algae oils is optimized. In the species development project, genetic engineering is used to create microalgae strains that are capable of high-level hydrocarbon production. For the modeling effort, the construction of multi-scaled models of algae production was prioritized, including integrating small-scale hydrodynamic models of algae production and reactor design and large-scale design optimization models.

  9. Exploratory Research - Using Volatile Organic Compounds to Separate Heterotrophic and Autotrophic Forest Soil Respiration

    SciTech Connect (OSTI)

    Roberts, Scott D; Hatten, Jeffrey A

    2015-02-09

    The initial focus of this project was to develop a method to partition soil respiration into its components (autotrophic, heterotrophic etc.) using the fingerprint of volatile organic compounds (VOCs) from soils. We were able to identify 63 different VOCs in our study; however, due to technical difficulties we were unable to take reliable measurements in order to test our hypotheses and develop this method. In the end, we changed the objectives of the project. Our new objectives were to characterize the effects of species and soil moisture regime on the composition of soil organic matter. We utilized the soils from the greenhouse experiment we had established for the soil VOC study and determined the lignin biomarker profiles of each of the treatments. We found that moisture had a significant effect on the carbon content of the soils with the low moisture treatments having higher carbon content than the high moisture treatments. We found that the relative yield of syringyl phenols (SP), ligin (Lig), and substituted fatty acids (SFA) were elevated in deciduous planted pots and reduced in conifer planted pots relative to plant-free treatments. Our results suggest nuttall oak preserved lignin and SFA, while loblolly pine lost lignin and SFA similarly to the plant free treatments. Since we did not find that the carbon concentrations of the soils were different between the species, nuttall oak probably replaced more native soil carbon than loblolly pine. This suggests that relative to loblolly pine, nuttall oak is a priming species. Since priming may impact soil carbon pools more than temperature or moisture, determining which species are priming species may facilitate an understanding of the interaction that land use and climate change may have on soil carbon pools.

  10. Method and apparatus using an active ionic liquid for algae biofuel harvest and extraction

    DOE Patents [OSTI]

    Salvo, Roberto Di; Reich, Alton; Dykes, Jr., H. Waite H.; Teixeira, Rodrigo

    2012-11-06

    The invention relates to use of an active ionic liquid to dissolve algae cell walls. The ionic liquid is used to, in an energy efficient manner, dissolve and/or lyse an algae cell walls, which releases algae constituents used in the creation of energy, fuel, and/or cosmetic components. The ionic liquids include ionic salts having multiple charge centers, low, very low, and ultra low melting point ionic liquids, and combinations of ionic liquids. An algae treatment system is described, which processes wet algae in a lysing reactor, separates out algae constituent products, and optionally recovers the ionic liquid in an energy efficient manner.

  11. Method to transform algae, materials therefor, and products produced thereby

    DOE Patents [OSTI]

    Dunahay, T.G.; Roessler, P.G.; Jarvis, E.E.

    1997-08-26

    Disclosed is a method to transform chlorophyll C-containing algae. The method includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further, specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae. 2 figs.

  12. Method to transform algae, materials therefor, and products produced thereby

    DOE Patents [OSTI]

    Dunahay, Terri Goodman; Roessler, Paul G.; Jarvis, Eric E.

    1997-01-01

    Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.

  13. Designer proton-channel transgenic algae for photobiological hydrogen production

    DOE Patents [OSTI]

    Lee, James Weifu

    2011-04-26

    A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H.sub.2) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal H.sub.2 productivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct (300) including a PCR forward primer (302), an externally inducible promoter (304), a transit targeting sequence (306), a designer proton-channel encoding sequence (308), a transcription and translation terminator (310), and a PCR reverse primer (312). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system (500) and a gas-products-separation and utilization system (600) for photobiological H.sub.2 production.

  14. Algae Biofuels Co-Location Assessment Tool for Canada

    Energy Science and Technology Software Center (OSTI)

    2011-11-29

    The Algae Biofuels Co-Location Assessment Tool for Canada uses chemical stoichiometry to estimate Nitrogen, Phosphorous, and Carbon atom availability from waste water and carbon dioxide emissions streams, and requirements for those same elements to produce a unit of algae. This information is then combined to find limiting nutrient information and estimate potential productivity associated with waste water and carbon dioxide sources. Output is visualized in terms of distributions or spatial locations. Distances are calculated betweenmore » points of interest in the model using the great circle distance equation, and the smallest distances found by an exhaustive search and sort algorithm.« less

  15. Algae Biofuels Co-Location Assessment Tool for Canada

    SciTech Connect (OSTI)

    2011-11-29

    The Algae Biofuels Co-Location Assessment Tool for Canada uses chemical stoichiometry to estimate Nitrogen, Phosphorous, and Carbon atom availability from waste water and carbon dioxide emissions streams, and requirements for those same elements to produce a unit of algae. This information is then combined to find limiting nutrient information and estimate potential productivity associated with waste water and carbon dioxide sources. Output is visualized in terms of distributions or spatial locations. Distances are calculated between points of interest in the model using the great circle distance equation, and the smallest distances found by an exhaustive search and sort algorithm.

  16. EERE Success Story-Sandia Algae Researchers Cut Costs with Improved...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling EERE Success Story-Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling October 19, 2015 - 3:40pm ...

  17. 3 Reasons We're Closer to an Algae Future than You Think | Department...

    Broader source: Energy.gov (indexed) [DOE]

    carbon-neutral. In addition, algae can grow in a variety of environments -- including man-made ponds, brackish water and wastewater. While algae shows great potential as a...

  18. California and New Mexico: Sapphire Energy Advances the Commercialization of Algae Crude Oil

    Broader source: Energy.gov [DOE]

    The Sapphire Green Crude Farm is the first algae-to-energy facility. If adopted and commercialized by other refineries, this algae-based crude oil is a viable green alternative fuel option.

  19. Algae-Based Biofuels: Applications and Co-Products | Open Energy...

    Open Energy Info (EERE)

    Algae-Based Biofuels: Applications and Co-Products Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Algae-Based Biofuels: Applications and Co-Products AgencyCompany...

  20. Top Five Things You Should Know About Algae | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Five Things You Should Know About Algae Top Five Things You Should Know About Algae November 6, 2013 - 2:40pm Addthis National Renewable Energy Laboratory researcher Lee Elliott collects samples of algae at a creek in Golden, Colorado. | Photo by Dennis Schroeder, National Renewable Energy Laboratory National Renewable Energy Laboratory researcher Lee Elliott collects samples of algae at a creek in Golden, Colorado. | Photo by Dennis Schroeder, National Renewable Energy Laboratory Christy

  1. Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrothermal Liquefaction | Department of Energy Whole Algae Hydrothermal Liquefaction Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae Hydrothermal Liquefaction Whole algae hydrothermal liquefaction is one of eight priority pathways chosen to convert biomass into hydrocarbon fuels by the Bioenergy Technologies Office. These pathways were down-selected from an initial list of 18. PDF icon Bioenergy Technologies Office Conversion R&D Pathway: Whole Algae Hydrothermal

  2. Switchable photosystem-II designer algae for photobiological hydrogen production

    DOE Patents [OSTI]

    Lee, James Weifu

    2010-01-05

    A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.

  3. EA-1829: Phycal Algae Pilot Project, Wahiawa and Kalaeloa, Hawaii

    Broader source: Energy.gov [DOE]

    This EA evaluates the environmental impacts of a proposal, through a cooperative agreement with Phycal, Inc., to partially fund implementing and evaluating new technology for the reuse of Carbon dioxide (CO2) emissions from industrial sources for green energy products. This project would use CO2 to grow algae for the production of algal oil and subsequent conversion to fuel.

  4. 3 Reasons We're Closer to an Algae Future than You Think | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy We're Closer to an Algae Future than You Think 3 Reasons We're Closer to an Algae Future than You Think July 29, 2015 - 12:35pm Addthis Paul Lester Paul Lester Digital Content Specialist, Office of Public Affairs KEY FACTS Algae shows great potential as a homegrown and renewable fuel source. The Bioenergy Technologies Office supports important research and development to make an algae future possible. Go to energy.gov/algae for more on the Energy Department's work related to algal

  5. mhtml:file://H:\CATX\APPROVED-CXS\EERE FOA 1201 - Rankine Cycle

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    . ., . . BNL-68599 PRODUCTION OF RADIOACTIVE IODINE David J. Schlyer Iodine-123 Probably the most 'widely used cyclotron produced radiohalogen is 1-123. It has gradually replaced I-13 1 as the isotope of choice for diagnostic radiopharmaceuticals containing radioiodine. It gives a much lower radiation dose to the patient and the gamma ray energy of 159 keV is ideally suited for use in a gamma camera. The gamma ray will penetrate tissue very effectively without excessive radiation dose. For this

  6. Sandia's Algae Nutrient Recycling Project Is a Triple Win

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Algae Nutrient Recycling Project Is a Triple Win - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste

  7. Sandia National Laboratories Algae Raceway Testing Facility Ribbon Cutting

    Broader source: Energy.gov [DOE]

    Sandia National Laboratories will be hosting a ribbon cutting on Feb. 4, 2016 at its Livermore Valley Open Campus to commemorate the opening of a new algae raceway testing facility. The new facility will allow researchers to better understand algal cultivation techniques, and is funded in part by the Bioenergy Technologies Office. Advanced Algal Systems Program Manager Alison Goss Eng and Technology Manager Daniel Fishman will be in attendance.

  8. Nitrogen control of 13C enrichment in heterotrophic organs relative to leaves in a landscape-building desert plant species

    SciTech Connect (OSTI)

    Zhang, J.; Gu, L.; Bao, F.; Cao, Y.; Hao, Y.; He, J.; Li, J.; Li, Y.; Ren, Y.; Wang, F.; Wu, R.; Yao, B.; Zhao, Y.; Lin, G.; Wu, B.; Lu, Q.; Meng, P.

    2014-09-10

    A longstanding puzzle in isotope studies of C3 plant species is that heterotrophic plant organs (e.g., stems, roots, seeds, and fruits) tend to be enriched in 13C compared to the autotrophic organ (leaves) that provides them with photosynthate. Our inability to explain this puzzle suggests key deficiencies in understanding post-photosynthetic metabolic processes. It also limits the effectiveness of applications of stable carbon isotope analyses in a variety of scientific disciplines ranging from plant physiology to global carbon cycle studies. To gain insight into this puzzle, we excavated whole plant architectures of Nitraria tangutorum Bobrov, a C3 species that has an exceptional capability of fixing sands and building sand dunes, in two deserts in northwestern China. We systematically and simultaneously measured carbon isotope ratios and nitrogen and phosphorous contents of different parts of the excavated plants. We also determined the seasonal variations in leaf carbon isotope ratios on nearby intact plants of N. tangutorum. We found, for the first time, that higher nitrogen contents in heterotrophic organs were significantly correlated with increased heterotrophic 13C enrichment compared to leaves. However, phosphorous contents had no effect on the enrichment. In addition, new leaves had carbon isotope ratios similar to roots but were progressively depleted in 13C as they matured. We concluded that a nitrogen-mediated process, probably the refixation of respiratory CO2 by phosphoenolpyruvate (PEP) carboxylase, was responsible for the differences in 13C enrichment among different heterotrophic organs while processes within leaves or during phloem loading may contribute to the overall autotrophic – heterotrophic difference in carbon isotope compositions.

  9. EERE Success Story-Sandia Algae Researchers Cut Costs with Improved

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nutrient Recycling | Department of Energy Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling EERE Success Story-Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling October 19, 2015 - 3:40pm Addthis Ryan Davis and Sandia National Laboratories colleagues have developed a method to recycle critical and costly algae cultivation nutrients phosphate and nitrogen. Photo by Dino Vournas. Ryan Davis and Sandia National Laboratories colleagues have developed a method

  10. Sandia algae raceway paves path from lab to real-world applications |

    National Nuclear Security Administration (NNSA)

    National Nuclear Security Administration Home / Blog Sandia algae raceway paves path from lab to real-world applications Tuesday, February 9, 2016 - 9:25am NNSA Blog Sandia California held a ribbon cutting ceremony for the Algae Raceway Testing Facility last week. The new facility will help scientists advance laboratory research to real-world applications. In a twist of geometry, an oval can make a line. The new algae raceway testing facility at Sandia National Laboratories in California may

  11. Science on the Hill: Driving toward an algae-powered future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science on the Hill: Driving toward an algae-powered future Science on the Hill: Driving toward an algae-powered future A new research project led by Los Alamos National Laboratory seeks to drive algal biofuels to marketability, decreasing our nation's dependence on fossil fuels and putting the brakes on global warming. December 24, 2015 LANL scientist Richard Sayre Los Alamos National Laboratory scientist David Fox holds a vial of blue-green algae that is part of the Laboratory's research into

  12. Algae Raceway Testing Facility Brings Algal Biofuels One Step Closer to Reality

    Broader source: Energy.gov [DOE]

    A new algae raceway testing facility opened earlier this month at Sandia National Laboratories in Livermore, California, that could help bring algal biofuels one step closer to commercialization....

  13. Study: Algae Could Replace 17% of U.S. Oil Imports | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Study: Algae Could Replace 17% of U.S. Oil Imports Study: Algae Could Replace 17% of U.S. Oil Imports April 13, 2011 - 6:30pm Addthis Algae samples back at the NREL lab, ready to be analyzed and run through the Fluorescent-Activated Cell Sorter, or FACS, which separates the cells. | Credit: NREL Staff Photographer Dennis Schroeder. Algae samples back at the NREL lab, ready to be analyzed and run through the Fluorescent-Activated Cell Sorter, or FACS, which separates the cells. | Credit: NREL

  14. Science on the Hill: Driving toward an algae-powered future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    These amazing - and amazingly prolific - photosynthetic microorganisms began pumping ... Algae's appetite for CO2 and their remarkable ability to produce oil might soon have us ...

  15. How ATP3 is Addressing the Challenges of Scale-up in Algae Technology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cal Poly Tryg Lundquist Braden Crowe Eric Nicolai Commercial Algae Management Albert Vitale Robert Vitale Georgia Tech Yongsheng Chen Steven Van Ginkel Thomas Igou Zixuan Hu ASU...

  16. Spectroradiometric monitoring for open outdoor culturing of algae and cyanobacteria

    SciTech Connect (OSTI)

    Reichardt, Thomas A.; Collins, Aaron M.; McBride, Robert C.; Behnke, Craig A.; Timlin, Jerilyn A.

    2014-08-20

    We assess the measurement of hyperspectral reflectance for the outdoor monitoring of green algae and cyanobacteria cultures with a multi-channel, fiber-coupled spectroradiometer. Reflectance data acquired over a four-week period are interpreted via numerical inversion of a reflectance model, in which the above-water reflectance is expressed as a quadratic function of the single backscattering albedo, dependent on the absorption and backscatter coefficients. The absorption coefficient is treated as the sum of component spectra consisting of the cultured species (green algae or cyanobacteria), dissolved organic matter, and water (including the temperature dependence of the water absorption spectrum). The backscatter coefficient is approximated as the scaled Hilbert transform of the culture absorption spectrum with a wavelength-independent vertical offset. Additional terms in the reflectance model account for the pigment fluorescence features and the water surface reflection of sunlight and skylight. For both the green algae and cyanobacteria, the wavelength-independent vertical offset of the backscatter coefficient is found to scale linearly with daily dry weight measurements, providing the capability for a non-sampling measurement of biomass in outdoor ponds. Other fitting parameters in the reflectance model are compared to auxiliary measurements and physics-based calculations. The magnitudes of the sunlight and skylight water-surface contributions derived from the reflectance model compare favorably with Fresnel reflectance calculations, while the reflectance-derived quantum efficiency of Chl-a fluorescence is found to be in agreement with literature values. To conlclude, the water temperature derived from the reflectance model exhibits excellent agreement with thermocouple measurements during the morning hours and highlights significantly elevated temperatures in the afternoon hours.

  17. Spectroradiometric monitoring for open outdoor culturing of algae and cyanobacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Reichardt, Thomas A.; Collins, Aaron M.; McBride, Robert C.; Behnke, Craig A.; Timlin, Jerilyn A.

    2014-08-20

    We assess the measurement of hyperspectral reflectance for the outdoor monitoring of green algae and cyanobacteria cultures with a multi-channel, fiber-coupled spectroradiometer. Reflectance data acquired over a four-week period are interpreted via numerical inversion of a reflectance model, in which the above-water reflectance is expressed as a quadratic function of the single backscattering albedo, dependent on the absorption and backscatter coefficients. The absorption coefficient is treated as the sum of component spectra consisting of the cultured species (green algae or cyanobacteria), dissolved organic matter, and water (including the temperature dependence of the water absorption spectrum). The backscatter coefficient is approximatedmore » as the scaled Hilbert transform of the culture absorption spectrum with a wavelength-independent vertical offset. Additional terms in the reflectance model account for the pigment fluorescence features and the water surface reflection of sunlight and skylight. For both the green algae and cyanobacteria, the wavelength-independent vertical offset of the backscatter coefficient is found to scale linearly with daily dry weight measurements, providing the capability for a non-sampling measurement of biomass in outdoor ponds. Other fitting parameters in the reflectance model are compared to auxiliary measurements and physics-based calculations. The magnitudes of the sunlight and skylight water-surface contributions derived from the reflectance model compare favorably with Fresnel reflectance calculations, while the reflectance-derived quantum efficiency of Chl-a fluorescence is found to be in agreement with literature values. To conlclude, the water temperature derived from the reflectance model exhibits excellent agreement with thermocouple measurements during the morning hours and highlights significantly elevated temperatures in the afternoon hours.« less

  18. Multi-scale Characterization of Improved Algae Strains

    SciTech Connect (OSTI)

    Dale, Taraka T.

    2015-04-01

    This report relays the important role biofuels such as algae could have in the energy market. The report cites that problem of crude oil becoming less abundant while the demand for energy continues to rise. There are many benefits of producing energy with biofuels such as fewer carbon emissions as well as less land area to produce the same amount of energy compared to other sources of renewable fuels. One challenge that faces biofuels right now is the cost to produce it is high.

  19. Algal Lipids and Omega-3 Production via Autotrophic and Heterotrophic Pathways at Cellana?s Kona Demonstration Facility, Hawaii

    SciTech Connect (OSTI)

    Bai, Xuemei; Knurek, Emily; Goes, Nikki; Griswold, Lynn

    2012-05-05

    Cellana?s Kona Demonstration Facility (KDF) is a 2.5 hectare facility, with 17,000 sq. ft. under roof and 1 hectare of cultivation systems. KDF is designed to execute and support all stages of the production process at pilot scale, from cultivation through extraction. Since Feb. 2009, KDF has been producing up to 0.7MT dry weight of algal biomass per month, while at the same time optimizing processes of cultivation, harvesting, dewatering and extraction. The cultivation system at KDF uses ALDUO? technology, a hybrid system of photobioreactors (PBRs) and open ponds. All fluid transfers related to KDF cultivation and harvesting processes are operated and monitored by a remote Process-Control System. Fluid transfer data, together with biochemical data, enable the mass balance calculations necessary to measure productivity. This poster summarizes methods to improve both biomass and lipids yield by 1) alleviating light limitation in open ponds, 2) de-oxygenation and 3) heterotrophic lipid production for post-harvesting cultures.

  20. Marine algae and land plants share conserved phytochrome signaling systems

    SciTech Connect (OSTI)

    Duanmu, Deqiang; Bachy, Charles; Sudek, Sebastian; Wong, Chee -Hong; Jimenez, Valeria; Rockwell, Nathan C.; Martin, Shelley S.; Ngan, Chew Yee; Reistetter, Emily N.; van Baren, Marijke J.; Price, Dana C.; Wei, Chia -Lin; Reyes-Prieto, Adrian; Lagarias, J. Clark; Worden, Alexandra Z.

    2014-09-29

    Phytochrome photosensors control a vast gene network in streptophyte plants, acting as master regulators of diverse growth and developmental processes throughout the life cycle. In contrast with their absence in known chlorophyte algal genomes and most sequenced prasinophyte algal genomes, a phytochrome is found in Micromonas pusilla, a widely distributed marine picoprasinophyte (<2 µm cell diameter). Together with phytochromes identified from other prasinophyte lineages, we establish that prasinophyte and streptophyte phytochromes share core light-input and signaling-output domain architectures except for the loss of C-terminal response regulator receiver domains in the streptophyte phytochrome lineage. Phylogenetic reconstructions robustly support the presence of phytochrome in the common progenitor of green algae and land plants. These analyses reveal a monophyletic clade containing streptophyte, prasinophyte, cryptophyte, and glaucophyte phytochromes implying an origin in the eukaryotic ancestor of the Archaeplastida. Transcriptomic measurements reveal diurnal regulation of phytochrome and bilin chromophore biosynthetic genes in Micromonas. The expression of these genes precedes both light-mediated phytochrome redistribution from the cytoplasm to the nucleus and increased expression of photosynthesis-associated genes. Prasinophyte phytochromes perceive wavelengths of light transmitted farther through seawater than the red/far-red light sensed by land plant phytochromes. Prasinophyte phytochromes also retain light-regulated histidine kinase activity lost in the streptophyte phytochrome lineage. Our studies demonstrate that light-mediated nuclear translocation of phytochrome predates the emergence of land plants and likely represents a widespread signaling mechanism in unicellular algae.

  1. Marine algae and land plants share conserved phytochrome signaling systems

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duanmu, Deqiang; Bachy, Charles; Sudek, Sebastian; Wong, Chee -Hong; Jimenez, Valeria; Rockwell, Nathan C.; Martin, Shelley S.; Ngan, Chew Yee; Reistetter, Emily N.; van Baren, Marijke J.; et al

    2014-09-29

    Phytochrome photosensors control a vast gene network in streptophyte plants, acting as master regulators of diverse growth and developmental processes throughout the life cycle. In contrast with their absence in known chlorophyte algal genomes and most sequenced prasinophyte algal genomes, a phytochrome is found in Micromonas pusilla, a widely distributed marine picoprasinophyte (<2 µm cell diameter). Together with phytochromes identified from other prasinophyte lineages, we establish that prasinophyte and streptophyte phytochromes share core light-input and signaling-output domain architectures except for the loss of C-terminal response regulator receiver domains in the streptophyte phytochrome lineage. Phylogenetic reconstructions robustly support the presence ofmore » phytochrome in the common progenitor of green algae and land plants. These analyses reveal a monophyletic clade containing streptophyte, prasinophyte, cryptophyte, and glaucophyte phytochromes implying an origin in the eukaryotic ancestor of the Archaeplastida. Transcriptomic measurements reveal diurnal regulation of phytochrome and bilin chromophore biosynthetic genes in Micromonas. The expression of these genes precedes both light-mediated phytochrome redistribution from the cytoplasm to the nucleus and increased expression of photosynthesis-associated genes. Prasinophyte phytochromes perceive wavelengths of light transmitted farther through seawater than the red/far-red light sensed by land plant phytochromes. Prasinophyte phytochromes also retain light-regulated histidine kinase activity lost in the streptophyte phytochrome lineage. Our studies demonstrate that light-mediated nuclear translocation of phytochrome predates the emergence of land plants and likely represents a widespread signaling mechanism in unicellular algae.« less

  2. June 2012 News Blast: Algae on the Mind | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 News Blast: Algae on the Mind June 2012 News Blast: Algae on the Mind Biomass Program monthly newsletter from June 2012. PDF icon june2012_newsblast.pdf More Documents & Publications Biomass Program Monthly News Blast - May 2012 July 2012 Biomass Program Monthly News Blast July 2012 Biomass Program

  3. BETO-Funded Algae Project at NREL Named a Finalist for 2015 R...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    BETO-Funded Algae Project at NREL Named a Finalist for 2015 R&D 100 Awards BETO-Funded Algae Project at NREL Named a Finalist for 2015 R&D 100 Awards August 13, 2015 - 4:44pm ...

  4. Discrete Event Modeling of Algae Cultivation and Harvesting at Commercial Scale: Capital Costs, Operating Costs, and System Bottlenecks

    SciTech Connect (OSTI)

    Lacey, Ph.D, P.E., Ronald E.

    2012-07-16

    Discrete Event Modeling of Algae Cultivation and Harvesting at Commercial Scale: Capital Costs, Operating Costs, and System Bottlenecks

  5. How ATP3 is Addressing the Challenges of Scale-up in Algae Technology R&D |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy How ATP3 is Addressing the Challenges of Scale-up in Algae Technology R&D How ATP3 is Addressing the Challenges of Scale-up in Algae Technology R&D Breakout Session 2-A: The Future of Algae-Based Biofuels How ATP3 is Addressing the Challenges of Scale-up in Algae Technology R&D Dr. John McGowen, Director of Operations and Program Manager, Arizona Center for Algae Technology and Innovation: Arizona State University PDF icon mcgowen_bioenergy_2015.pdf More

  6. ENERGY PRODUCTIVITY OF THE HIGH VELOCITY ALGAE RACEWAY INTEGRATED DESIGN (ARID-HV)

    SciTech Connect (OSTI)

    Attalah, Said; Waller, Peter; Khawam, G.; Ryan, Randy; Huesemann, Michael H.

    2015-01-31

    The original Algae Raceway Integrated Design (ARID) raceway was an effective method to increase algae culture temperature in open raceways. However, the energy input was high and flow mixing was poor. Thus, the High Velocity Algae Raceway Integrated Design (ARID-HV) raceway was developed to reduce energy input requirements and improve flow mixing in a serpentine flow path. A prototype ARID-HV system was installed in Tucson, Arizona. Based on algae growth simulation and hydraulic analysis, an optimal ARID-HV raceway was designed, and the electrical energy input requirement (kWh ha-1 d-1) was calculated. An algae growth model was used to compare the productivity of ARIDHV and conventional raceways. The model uses a pond surface energy balance to calculate water temperature as a function of environmental parameters. Algae growth and biomass loss are calculated based on rate constants during day and night, respectively. A 10 year simulation of DOE strain 1412 (Chlorella sorokiniana) showed that the ARID-HV raceway had significantly higher production than a conventional raceway for all months of the year in Tucson, Arizona. It should be noted that this difference is species and climate specific and is not observed in other climates and with other algae species. The algae growth model results and electrical energy input evaluation were used to compare the energy productivity (algae production rate/energy input) of the ARID-HV and conventional raceways for Chlorella sorokiniana in Tucson, Arizona. The energy productivity of the ARID-HV raceway was significantly greater than the energy productivity of a conventional raceway for all months of the year.

  7. World's First Algae Surfboard Makes Waves in San Diego | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy World's First Algae Surfboard Makes Waves in San Diego World's First Algae Surfboard Makes Waves in San Diego April 23, 2015 - 4:15pm Addthis On the eve of Earth Day, UCSD unveiled their new innovative and sustainable algae surfboard at the San Diego Symphony Hall. San Diego Mayor Kevin Faulconer, Marty Gilchrist of Arctic Foam, Steve Mayfield of Cal-CAB, and champion surfer Rob Machado discuss the development of the "surfboard of the future." Credit: UC San Diego

  8. Re-utilization of Industrial CO2 for Algae Production Using a Phase Change Material

    SciTech Connect (OSTI)

    Joseph, Brian

    2013-12-31

    This is the final report of a 36-month Phase II cooperative agreement. Under this project, Touchstone Research Laboratory (Touchstone) investigated the merits of incorporating a Phase Change Material (PCM) into an open-pond algae production system that can capture and re-use the CO2 from a coal-fired flue gas source located in Wooster, OH. The primary objective of the project was to design, construct, and operate a series of open algae ponds that accept a slipstream of flue gas from a coal-fired source and convert a significant portion of the CO2 to liquid biofuels, electricity, and specialty products, while demonstrating the merits of the PCM technology. Construction of the pilot facility and shakedown of the facility in Wooster, OH, was completed during the first two years, and the focus of the last year was on operations and the cultivation of algae. During this Phase II effort a large-scale algae concentration unit from OpenAlgae was installed and utilized to continuously harvest algae from indoor raceways. An Algae Lysing Unit and Oil Recovery Unit were also received and installed. Initial parameters for lysing nanochloropsis were tested. Conditions were established that showed the lysing operation was effective at killing the algae cells. Continuous harvesting activities yielded over 200 kg algae dry weight for Ponds 1, 2 and 4. Studies were conducted to determine the effect of anaerobic digestion effluent as a nutrient source and the resulting lipid productivity of the algae. Lipid content and total fatty acids were unaffected by culture system and nutrient source, indicating that open raceway ponds fed diluted anaerobic digestion effluent can obtain similar lipid productivities to open raceway ponds using commercial nutrients. Data were also collected with respect to the performance of the PCM material on the pilot-scale raceway ponds. Parameters such as evaporative water loss, temperature differences, and growth/productivity were tracked. The pond with the PCM material was consistently 2 to 5°C warmer than the control pond. This difference did not seem to increase significantly over time. During phase transitions for the PCM, the magnitude of the difference between the daily minimum and maximum temperatures decreased, resulting in smaller daily temperature fluctuations. A thin layer of PCM material reduced overall water loss by 74% and consistently provided algae densities that were 80% greater than the control pond.

  9. "The Promise and Challenge of Algae as Renewable Sources of Biofuels"

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9-8-2010 - Transcript | Department of Energy "The Promise and Challenge of Algae as Renewable Sources of Biofuels" 9-8-2010 - Transcript "The Promise and Challenge of Algae as Renewable Sources of Biofuels" 9-8-2010 - Transcript This focused on the Office's approach to algal biofuels research and development and included presentations from four representatives of its recently funded consortia. This session also discussed highlights from the National Algal Biofuels

  10. Renewable Fuels from Algae Boosted by NREL Refinery Process - News Releases

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | NREL Renewable Fuels from Algae Boosted by NREL Refinery Process February 9, 2016 A new biorefinery process developed by scientists at the Energy Department's National Renewable Energy Laboratory (NREL) has proven to be significantly more effective at producing ethanol from algae than previous research. The process, dubbed Combined Algal Processing (CAP), is detailed in a new paper by NREL's Tao Dong, Eric Knoshaug, Ryan Davis, Lieve Laurens, Stefanie Van Wychen, Philip Pienkos, and Nick

  11. Nanotechnology and algae biofuels exhibits open July 26 at the Bradbury

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science Museum Nanotechnology and algae biofuels exhibits open July 26 Nanotechnology and algae biofuels exhibits open July 26 at the Bradbury Science Museum The Bradbury Science Museum is opening two new exhibits as part of the Laboratory's 70th Anniversary celebration. July 22, 2013 What if you could power your life using pond scum? Los Alamos researchers are working to make this a reality. What if you could power your life using pond scum? Los Alamos researchers are working to make this a

  12. Algal Biology Toolbox Workshop Brings Lead Experts to Inform Algae-Based Biofuel Strategy

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy’s (DOE’s) Bioenergy Technologies Office (BETO) is hosting a two-day workshop gathering lead experts in the field of algal biology from May 24–25, 2016. This workshop, “Sharpening Our Tools: Algal Biology Toolbox Workshop,” held in San Diego, California, will discuss research and development (R&D) needed to achieve affordable, scalable, and sustainable algae-based biofuels. It is the first algal biofuels strategy workshop to focus specifically on improvements in algal biology—a key research focus required to advance the economic viability of algae-based biofuels.

  13. ALDUO(TM) Algae Cultivation Technology for Delivering Sustainable Omega-3s, Feed, and Fuel

    SciTech Connect (OSTI)

    Bai, Xuemei

    2012-09-24

    * ALDUO(TM) Algae Production Technology Cellana?s Proprietary, Photosynthetic, & Proven * ALDUO(TM) Enables Economic Algae Production Unencumbered by Contamination by Balancing Higher-Cost PBRs with Lower-Cost Open Ponds * ALDUO(TM) Advantages * ALDUO(TM) Today o Large collection of strains for high value co-products o Powerful Mid-scale Screening & Optimization System o Solution to a Conflicting Interest o Split Pond Yield Enhancement o Heterotrophy & mixotrophy as a "finishing step" o CO2 Mitigation-flue Gas Operation o Worldwide Feed Trials with Livestock & Aquatic Species * ALDUO(TM) Technology Summarized

  14. Catalytic Hydrothermal Gasification of Lignin-Rich Biorefinery Residues and Algae Final Report

    SciTech Connect (OSTI)

    Elliott, Douglas C.; Neuenschwander, Gary G.; Hart, Todd R.; Rotness, Leslie J.; Zacher, Alan H.; Santosa, Daniel M.; Valkenburt, Corinne; Jones, Susanne B.; Tjokro Rahardjo, Sandra A.

    2009-11-03

    This report describes the results of the work performed by PNNL using feedstock materials provided by the National Renewable Energy Laboratory, KL Energy and Lignol lignocellulosic ethanol pilot plants. Test results with algae feedstocks provided by Genifuel, which provided in-kind cost share to the project, are also included. The work conducted during this project involved developing and demonstrating on the bench-scale process technology at PNNL for catalytic hydrothermal gasification of lignin-rich biorefinery residues and algae. A technoeconomic assessment evaluated the use of the technology for energy recovery in a lignocellulosic ethanol plant.

  15. Uptake and Retention of Cs137 by a Blue-Green Alga in Continuous Flow and Batch Culture Systems

    SciTech Connect (OSTI)

    Watts, J.R.

    2003-02-18

    Since routine monitoring data show that blue-green algae concentrate radioactivity from water by factors as great as 10,000, this study was initiated to investigate the uptake and retention patterns of specific radionuclides by the dominant genera of blue-green algae in the reactor effluents. Plectonema purpureum was selected for this study.

  16. The Dark Side of Algae Cultivation. Characterizing night biomass loss in three photosynthetic algae, Chlorella sorokiniana, Nannochloropsis salina and Picochlorum sp

    SciTech Connect (OSTI)

    Edmundson, Scott J.; Huesemann, Michael H.

    2015-10-28

    Night biomass loss in photosynthetic algae is an essential parameter that is often overlooked when modeling or optimizing biomass productivities. Night respiration acts as a tax on daily biomass gains and has not been well characterized in the context of biofuel production. We examined the night biomass loss in three algae strains that may have potential for commercial biomass production (Nannochloropsis salina- CCMP1776, Chlorella sorokiniana- DOE1412, and Picochlorum sp. LANL-WT). Biomass losses were monitored by ash free dry weight (AFDW mg/L-1) and optical density (OD750) on a thermal-gradient incubator. Night biomass loss rates were highly variable (ranging from -0.006 to -0.59 day -1), species-specific, and dependent on both culture growth phase prior to the dark period and night pond temperature. In general, the fraction of biomass lost over a 10 hour dark period, which ranged from ca. 1 to 22% in our experiments, was positively correlated with temperature and declined as the culture transitioned from exponential to linear to stationary phase. The dynamics of biomass loss should be taken into consideration in algae strain selection, are critical in predictive modeling of biomass production based on geographic location and can influence the net productivity of photosynthetic cultures used for bio-based fuels or products.

  17. Algae: The Source of Reliable, Scalable, and Sustainable Liquid Transportation Fuels

    Broader source: Energy.gov [DOE]

    At the February 12, 2009 joint Web conference of DOE's Biomass and Clean Cities programs, Brian Goodall (Sapphire Energy) spoke on Continental Airlines’ January 7th Biofuels Test. The flight was fueled, in part, by Sapphire’s algae-based jet fuel.

  18. EERE Assistant Secretary and BETO Director Confirmed Speakers for Algae Biomass Summit

    Broader source: Energy.gov [DOE]

    Dr. David Danielson, Assistant Secretary of the Office of Energy Efficiency and Renewable Energy and Jonathan Male, Director of the Bioenergy Technologies Office, will be speaking at the Algae Biomass Summit, September 29–October 2, 2014 in San Diego, California.

  19. Algae Biofuels Collaborative Project: Cooperative Research and Development Final Report, CRADA Number CRD-10-371

    SciTech Connect (OSTI)

    French, R. J.

    2012-04-01

    The goal of this project is to advance biofuels research on algal feedstocks and NREL's role in the project is to explore novel liquid extraction methods, gasification and pyrolysis as means to produce fuels from algae. To that end several different extraction methods were evaluated and numerous gasification and pyrolysis conditions were explored. It was found that mild hydrothermal treatment is a promising means to improve the extraction and conversion of lipids from algae over those produced by standard extraction methods. The algae were essentially found to gasify completely at a fairly low temperature of 750 degrees C in the presence of oxygen. Pyrolysis from 300-550 degrees C showed sequential release of phytene hydrocarbons, glycerides, and aromatics as temperature was increased. It appears that this has potential to release the glycerides from the non-fatty acid groups present in the polar lipids to produce a cleaner lipid. Further research is needed to quantify the pyrolysis and gasification yields, analyze the liquids produced and to test strategies for removing organic-nitrogen byproducts produced because of the high protein content of the feed. Possible strategies include use of high-lipid/low-protein algae or the use of catalytic pyrolysis.

  20. Bioenergy Technologies Office R&D with University of California San Diego Results in First Algae Surfboard

    Broader source: Energy.gov [DOE]

    Fossil-fuel derived polyurethane products may not be in demand in the coming years thanks to algae researchers. A joint research and development investment from the Energy Departments Bioenergy...

  1. PYOMELANIN IS PRODUCED BY SHEWANELLA ALGAE BRY AND EFFECTED BY EXOGENOUS IRON

    SciTech Connect (OSTI)

    Turick, C; Frank Caccavo, F; Jr., J; Louis S. Tisa, L

    2006-11-29

    Melanin production by S. algae BrY occurred during late/post-exponential growth in lactate-basal-salts liquid medium supplemented with tyrosine or phenylalanine. The antioxidant ascorbate inhibited melanin production, but not production of the melanin precursor, homogentisic acid. In the absence of ascorbate, melanin production was inhibited by the 4-hydroxyplenylpyruvate dioxygenase inhibitor, sulcotrione and Fe(II) (>0.2mM). These data support the hypothesis that pigment production by S. algae BrY was a result the conversion of tyrosine or phenylalanine to homogentisic acid which was excreted, auto-oxidized and self-polymerized to form pyomelanin. The inverse relationship between Fe(II) concentration and pyomelanin production has implications that pyomelanin may play a role in iron assimilation under Fe(II) limiting conditions.

  2. The Promise and Challenge of Algae as Renewable Sources of Biofuels

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy eere.energy.gov 1 Program Name or Ancillary Text eere.energy.gov The Promise and Challenge of Algae as Renewable Sources of Biofuels Biomass Program Webinar September 8, 2010 Joanne Morello and Ron Pate DOE-EERE-Office of Biomass Program Webinar Outline 1. Introduction to DOE Biomass Program and our emerging algal biofuels initiative (25 minutes) 2. Overview of DOE's National Algal Biofuels Technology Roadmap: defining the algal biofuels supply chain and the remaining R&D challenges

  3. Lipid Extraction from Wet-Algae for Biofuel Production - Energy Innovation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portal Biomass and Biofuels Biomass and Biofuels Advanced Materials Advanced Materials Find More Like This Return to Search Lipid Extraction from Wet-Algae for Biofuel Production University of Colorado Contact CU About This Technology Technology Marketing SummaryThere is a growing interest in algal biofuels; however, current methods of a thermal separation process for solvent mixtures involve concomitant issues and increased energy consumption. A research team at the University of Colorado

  4. Extensive horizontal gene transfer, duplication, and loss of chlorophyll synthesis genes in the algae

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hunsperger, Heather M.; Randhawa, Tejinder; Cattolico, Rose Ann

    2015-02-10

    Two non-homologous, isofunctional enzymes catalyze the penultimate step of chlorophyll a synthesis in oxygenic photosynthetic organisms such as cyanobacteria, eukaryotic algae and land plants: the light independent (LIPOR) and light-dependent (POR) protochlorophyllide oxidoreductases. Whereas the distribution of these enzymes in cyanobacteria and land plants is well understood, the presence, loss, duplication, and replacement of these genes have not been surveyed in the polyphyletic and remarkably diverse eukaryotic algal lineages.

  5. Process Development for Hydrothermal Liquefaction of Algae Feedstocks in a Continuous-Flow Reactor

    SciTech Connect (OSTI)

    Elliott, Douglas C.; Hart, Todd R.; Schmidt, Andrew J.; Neuenschwander, Gary G.; Rotness, Leslie J.; Olarte, Mariefel V.; Zacher, Alan H.; Albrecht, Karl O.; Hallen, Richard T.; Holladay, Johnathan E.

    2013-10-01

    Wet algae slurries can be converted into an upgradeable biocrude by hydrothermal liquefaction (HTL). High levels of carbon conversion to gravity-separable biocrude product were accomplished at relatively low temperature (350 ?C) in a continuous-flow, pressurized (sub-critical liquid water) environment (20 MPa). As opposed to earlier work in batch reactors reported by others, direct oil recovery was achieved without the use of a solvent and biomass trace components were removed by processing steps so that they did not cause process difficulties. High conversions were obtained even with high slurry concentrations of up to 35 wt% of dry solids. Catalytic hydrotreating was effectively applied for hydrodeoxygenation, hydrodenitrogenation, and hydrodesulfurization of the biocrude to form liquid hydrocarbon fuel. Catalytic hydrothermal gasification was effectively applied for HTL byproduct water cleanup and fuel gas production from water soluble organics, allowing the water to be considered for recycle of nutrients to the algae growth ponds. As a result, high conversion of algae to liquid hydrocarbon and gas products was found with low levels of organic contamination in the byproduct water. All three process steps were accomplished in bench-scale, continuous-flow reactor systems such that design data for process scale-up was generated.

  6. Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri

    SciTech Connect (OSTI)

    Prochnik, Simon E.; Umen, James; Nedelcu, Aurora; Hallmann, Armin; Miller, Stephen M.; Nishii, Ichiro; Ferris, Patrick; Kuo, Alan; Mitros, Therese; Fritz-Laylin, Lillian K.; Hellsten, Uffe; Chapman, Jarrod; Simakov, Oleg; Rensing, Stefan A.; Terry, Astrid; Pangilinan, Jasmyn; Kapitonov, Vladimir; Jurka, Jerzy; Salamov, Asaf; Shapiro, Harris; Schmutz, Jeremy; Grimwood, Jane; Lindquist, Erika; Lucas, Susan; Grigoriev, Igor V.; Schmitt, Rudiger; Kirk, David; Rokhsar, Daniel S.

    2010-07-01

    Analysis of the Volvox carteri genome reveals that this green alga's increased organismal complexity and multicellularity are associated with modifications in protein families shared with its unicellular ancestor, and not with large-scale innovations in protein coding capacity. The multicellular green alga Volvox carteri and its morphologically diverse close relatives (the volvocine algae) are uniquely suited for investigating the evolution of multicellularity and development. We sequenced the 138 Mb genome of V. carteri and compared its {approx}14,500 predicted proteins to those of its unicellular relative, Chlamydomonas reinhardtii. Despite fundamental differences in organismal complexity and life history, the two species have similar protein-coding potentials, and few species-specific protein-coding gene predictions. Interestingly, volvocine algal-specific proteins are enriched in Volvox, including those associated with an expanded and highly compartmentalized extracellular matrix. Our analysis shows that increases in organismal complexity can be associated with modifications of lineage-specific proteins rather than large-scale invention of protein-coding capacity.

  7. Developing New Alternative Energy in Virginia: Bio-Diesel from Algae

    SciTech Connect (OSTI)

    Hatcher, Patrick

    2012-03-29

    The overall objective of this study was to select chemical processing equipment, install and operate that equipment to directly convert algae to biodiesel via a reaction patented by Old Dominion University (Pat. No. US 8,080,679B2). This reaction is a high temperature (250- 330{degrees}C) methylation reaction utilizing tetramethylammonium hydroxide (TMAH) to produce biodiesel. As originally envisioned, algal biomass could be treated with TMAH in methanol without the need to separately extract triacylglycerides (TAG). The reactor temperature allows volatilization and condensation of the methyl esters whereas the spent algae solids can be utilized as a high-value fertilizer because they are minimally charred. During the course of this work and immediately prior to commencing, we discovered that glycerol, a major by-product of the conventional transesterification reaction for biofuels, is not formed but rather three methoxylated glycerol derivatives are produced. These derivatives are high-value specialty green chemicals that strongly upgrade the economics of the process, rendering this approach as one that now values the biofuel only as a by-product, the main value products being the methoxylated glycerols. A horizontal agitated thin-film evaporator (one square foot heat transfer area) proved effective as the primary reactor facilitating the reaction and vaporization of the products, and subsequent discharge of the spent algae solids that are suitable for supplementing petrochemicalbased fertilizers for agriculture. Because of the size chosen for the reactor, we encountered problems with delivery of the algal feed to the reaction zone, but envision that this problem could easily disappear upon scale-up or can be replaced economically by incorporating an extraction process. The objective for production of biodiesel from algae in quantities that could be tested could not be met, but we implemented use of soybean oil as a surrogate TAG feed to overcome this limitation. The positive economics of this process are influenced by the following: 1. the weight percent of dry algae in suspension that can be fed into the evaporator, 2. the alga species’ ability to produce a higher yield of biodiesel, 3. the isolation of valuable methoxylated by-products, 4. recycling and regeneration of methanol and TMAH, and 5. the market value of biodiesel, commercial agricultural fertilizer, and the three methoxylated by-products. The negative economics of the process are the following: 1. the cost of producing dried, ground algae, 2. the capital cost of the equipment required for feedstock mixing, reaction, separation and recovery of products, and reactant recycling, and 3. the electrical cost and other utilities. In this report, the economic factors and results are assembled to predict the commercialization cost and its viability. This direct conversion process and equipment discussed herein can be adapted for various feedstocks including: other algal species, vegetable oil, jatropha oil, peanut oil, sunflower oil, and other TAG containing raw materials as a renewable energy resource.

  8. Chemical Processing in High-Pressure Aqueous Environments. 9. Process Development for Catalytic Gasification of Algae Feedstocks

    SciTech Connect (OSTI)

    Elliott, Douglas C.; Hart, Todd R.; Neuenschwander, Gary G.; Rotness, Leslie J.; Olarte, Mariefel V.; Zacher, Alan H.

    2012-07-26

    Through the use of a metal catalyst, gasification of wet algae slurries can be accomplished with high levels of carbon conversion to gas at relatively low temperature (350 C). In a pressurized-water environment (20 MPa), near-total conversion of the organic structure of the algae to gases has been achieved in the presence of a supported ruthenium metal catalyst. The process is essentially steam reforming, as there is no added oxidizer or reagent other than water. In addition, the gas produced is a medium-heating value gas due to the synthesis of high levels of methane, as dictated by thermodynamic equilibrium. As opposed to earlier work, biomass trace components were removed by processing steps so that they did not cause processing difficulties in the fixed catalyst bed tubular reactor system. As a result, the algae feedstocks, even those with high ash contents, were much more reliably processed. High conversions were obtained even with high slurry concentrations. Consistent catalyst operation in these short-term tests suggested good stability and minimal poisoning effects. High methane content in the product gas was noted with significant carbon dioxide captured in the aqueous byproduct in combination with alkali constituents and the ammonia byproduct derived from proteins in the algae. High conversion of algae to gas products was found with low levels of byproduct water contamination and low to moderate loss of carbon in the mineral separation step.

  9. Surface complexation of neptunium (V) onto whole cells and cell componets of Shewanella alga

    SciTech Connect (OSTI)

    Reed, Donald Timothy; Deo, Randhir P; Rittmann, Bruce E; Songkasiri, Warinthorn

    2008-01-01

    We systematically quantified surface complexation of neptunium(V) onto whole cells of Shewanella alga strain BrY and onto cell wall and extracellular polymeric substances (EPS) of S. alga. We first performed acid and base titrations and used the mathematical model FITEQL with constant-capacitance surface-complexation to determine the concentrations and deprotonation constants of specific surface functional groups. Deprotonation constants most likely corresponded to a carboxyl site associated with amino acids (pK{sub a} {approx} 2.4), a carboxyl group not associated with amino acids (pK{sub a} {approx} 5), a phosphoryl site (pK{sub a} {approx} 7.2), and an amine site (pK{sub a} > 10). We then carried out batch sorption experiments with Np(V) and each of the S. alga components at different pHs. Results show that solution pH influenced the speciation of Np(V) and each of the surface functional groups. We used the speciation sub-model of the biogeochemical model CCBATCH to compute the stability constants for Np(V) complexation to each surface functional group. The stability constants were similar for each functional group on S. alga bacterial whole cells, cell walls, and EPS, and they explain the complicated sorption patterns when they are combined with the aqueous-phase speciation of Np(V). For pH < 8, NpO{sub 2}{sup +} was the dominant form of Np(V), and its log K values for the low-pK{sub a} carboxyl, other carboxyl, and phosphoryl groups were 1.75, 1.75, and 2.5 to 3.1, respectively. For pH greater than 8, the key surface ligand was amine >XNH3+, which complexed with NpO{sub 2}(CO{sub 3}){sub 3}{sup 5-}. The log K for NpO{sub 2}(CO{sub 3}){sub 3}{sup 5-} complexed onto the amine groups was 3.1 to 3.6. All of the log K values are similar to those of Np(V) complexes with aqueous carboxyl and N-containing carboxyl ligands. These results point towards the important role of surface complexation in defining key actinide-microbiological interactions in the subsurface.

  10. Updated Cost Analysis of Photobiological Hydrogen Production from Chlamydomonas reinhardtii Green Algae: Milestone Completion Report

    SciTech Connect (OSTI)

    Amos, W. A.

    2004-01-01

    This report updates the 1999 economic analysis of NREL's photobiological hydrogen production from Chlamydomonas reinhardtii. The previous study had looked mainly at incident light intensities, batch cycles and light adsorption without directly attempting to model the saturation effects seen in algal cultures. This study takes a more detailed look at the effects that cell density, light adsorption and light saturation have on algal hydrogen production. Performance estimates based on actual solar data are also included in this study. Based on this analysis, the estimated future selling price of hydrogen produced from algae ranges $0.57/kg to $13.53/kg.

  11. Algae Biotechnology

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... 8 NEW CS 91 99 8 1 2 0 0 1 0 CSI-V10 10 JO MIX 0 1 0 88 99 11 0 0 0 CSI2012 2012 DNA 81 99 25 0 4 1 Genome-based Culture Diagnostics 42 Induction of lipid and TAG ...

  12. Structure and scintillation yield of Ce-doped AlGa substituted yttrium garnet

    SciTech Connect (OSTI)

    Sidletskiy, Oleg; Kononets, Valerii; Lebbou, Kheirreddine; Neicheva, Svetlana; Voloshina, Olesya; Bondar, Valerii; Baumer, Vyacheslav; Belikov, Konstantin; Gektin, Alexander; Grinyov, Boris; Joubert, Marie-France

    2012-11-15

    Highlights: ? Range of Y{sub 3}(Al{sub 1?x}Ga{sub x}){sub 5}O{sub 12}:Ce solid solution crystals are grown from melt by the Czochralski method. ? Light yield of mixed crystals reaches 130% of the YAG:Ce value at x ? 0.4. ? ?1% of antisite defects is formed in YGG:Ce, but no evidence of this is obtained for the rest of crystals. -- Abstract: Structure and scintillation yield of Y{sub 3}(Al{sub 1?x}Ga{sub x}){sub 5}O{sub 12}:Ce solid solution crystals are studied. Crystals are grown from melt by the Czochralski method. Distribution of host cations in crystal lattice is determined. Quantity of antisite defects in crystals is evaluated using XRD and atomic emission spectroscopy data. Trend of light output at Al/Ga substitution in Y{sub 3}(Al{sub 1?x}Ga{sub x}){sub 5}O{sub 12}:Ce is determined for the first time. Light output in mixed crystals reaches 130% comparative to Ce-doped yttriumaluminum garnet. Luminescence properties at Al/Ga substitution are evaluated.

  13. 2010-09-08 14.03 The Promise and Challenges of Algae as a Renewable Sources of Biofuels

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    "The Promise and Challenge of Algae as Renewable Sources of Biofuels" 9-8-2010 Joanne Morello: Today we're going to be talking about, as you know, "The Promise and Challenge of Algae as Renewable Sources of Biofuels," but I do want to note that it is the first in a series, we're hoping, of educational and outreach style Webinars to inform people about the different technology areas that we focus on in the Biomass Program. The topic of the next Webinar is to be determined, but

  14. BETO-Funded Algae Project at NREL Named a Finalist for 2015 R&D 100 Awards

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy BETO-Funded Algae Project at NREL Named a Finalist for 2015 R&D 100 Awards BETO-Funded Algae Project at NREL Named a Finalist for 2015 R&D 100 Awards August 13, 2015 - 4:44pm Addthis Cyanobacteria cultures. Photo by Dennis Schroeder/NREL. Cyanobacteria cultures. Photo by Dennis Schroeder/NREL. The R&D 100 Awards, presented annually by R&D Magazine, recognize 100 of the most innovative technologies and services of the year across nine categories and are

  15. Process Design and Economics for the Conversion of Algal Biomass to Hydrocarbons: Whole Algae Hydrothermal Liquefaction and Upgrading

    SciTech Connect (OSTI)

    Jones, Susanne B.; Zhu, Yunhua; Anderson, Daniel B.; Hallen, Richard T.; Elliott, Douglas C.; Schmidt, Andrew J.; Albrecht, Karl O.; Hart, Todd R.; Butcher, Mark G.; Drennan, Corinne; Snowden-Swan, Lesley J.; Davis, Ryan; Kinchin, Christopher

    2014-03-20

    This report provides a preliminary analysis of the costs associated with converting whole wet algal biomass into primarily diesel fuel. Hydrothermal liquefaction converts the whole algae into an oil that is then hydrotreated and distilled. The secondary aqueous product containing significant organic material is converted to a medium btu gas via catalytic hydrothermal gasification.

  16. Chromatin landscaping in algae reveals novel regulation pathway for biofuels production

    SciTech Connect (OSTI)

    Ngan, Chew Yee; Wong, Chee-Hong; Choi, Cindy; Pratap, Abhishek; Han, James; Wei, Chia-Lin

    2013-02-19

    The diminishing reserve of fossil fuels calls for the development of biofuels. Biofuels are produced from renewable resources, including photosynthetic organisms, generating clean energy. Microalgae is one of the potential feedstock for biofuels production. It grows easily even in waste water, and poses no competition to agricultural crops for arable land. However, little is known about the algae lipid biosynthetic regulatory mechanisms. Most studies relied on the homology to other plant model organisms, in particular Arabidopsis or through low coverage expression analysis to identify key enzymes. This limits the discovery of new components in the biosynthetic pathways, particularly the genetic regulators and effort to maximize the production efficiency of algal biofuels. Here we report an unprecedented and de novo approach to dissect the algal lipid pathways through disclosing the temporal regulations of chromatin states during lipid biosynthesis. We have generated genome wide chromatin maps in chlamydomonas genome using ChIP-seq targeting 7 histone modifications and RNA polymerase II in a time-series manner throughout conditions activating lipid biosynthesis. To our surprise, the combinatory profiles of histone codes uncovered new regulatory mechanism in gene expression in algae. Coupled with matched RNA-seq data, chromatin changes revealed potential novel regulators and candidate genes involved in the activation of lipid accumulations. Genetic perturbation on these candidate regulators further demonstrated the potential to manipulate the regulatory cascade for lipid synthesis efficiency. Exploring epigenetic landscape in microalgae shown here provides powerful tools needed in improving biofuel production and new technology platform for renewable energy generation, global carbon management, and environmental survey.

  17. A lipid-accumulating alga maintains growth in outdoor, alkaliphilic raceway pond with mixed microbial communities

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bell, Tisza A.S.; Prithiviraj, Bharath; Wahlen, Brad D.; Fields, Matthew W.; Peyton, Brent M.

    2016-01-07

    Algal biofuels and valuable co-products are being produced in both open and closed cultivation systems. Growing algae in open pond systems may be a more economical alternative, but this approach allows environmental microorganisms to colonize the pond and potentially infect or outcompete the algal “crop.” In this study, we monitored the microbial community of an outdoor, open raceway pond inoculated with a high lipid-producing alkaliphilic alga, Chlorella vulgaris BA050. The strain C. vulgaris BA050 was previously isolated from Soap Lake, Washington, a system characterized by a high pH (~9.8). An outdoor raceway pond (200 L) was inoculated with C. vulgarismore » and monitored for 10 days and then the culture was transferred to a 2,000 L raceway pond and cultivated for an additional 6 days. Community DNA samples were collected over the 16-day period in conjunction with water chemistry analyses and cell counts. Universal primers for the SSU rRNA gene sequences for Eukarya, Bacteria, and Archaea were used for barcoded pyrosequence determination. The environmental parameters that most closely correlated with C. vulgaris abundance were pH and phosphate. Community analyses indicated that the pond system remained dominated by the Chlorella population (93% of eukaryotic sequences), but was also colonized by other microorganisms. Bacterial sequence diversity increased over time while archaeal sequence diversity declined over the same time period. Using SparCC co-occurrence network analysis, a positive correlation was observed between C. vulgaris and Pseudomonas sp. throughout the experiment, which may suggest a symbiotic relationship between the two organisms. The putative relationship coupled with high pH may have contributed to the success of C. vulgaris. As a result, the characterization of the microbial community dynamics of an alkaliphilic open pond system provides significant insight into open pond systems that could be used to control photoautotrophic biomass productivity in an open, non-sterile environment.« less

  18. Investigating Sources of Toxicity in Stormwater: Algae Mortality in Runoff Upstream of the Lawrence Livermore National Laboratory

    SciTech Connect (OSTI)

    Campbell, C G; Folks, K; Mathews, S; Martinelli, R

    2003-10-06

    A source evaluation case study is presented for observations of algae toxicity in an intermittent stream passing through the Lawrence Livermore National Laboratory near Livermore, California. A five-step procedure is discussed to determine the cause of water toxicity problems and to determine appropriate environmental management practices. Using this approach, an upstream electrical transfer station was identified as the probable source of herbicides causing the toxicity. In addition, an analytical solution for solute transport in overland flow was used to estimate the application level of 40 Kg/ha. Finally, this source investigation demonstrates that pesticides can impact stream water quality regardless of application within levels suggested on manufacturer labels. Environmental managers need to ensure that pesticides that could harm aquatic organisms (including algae) not be used within close proximity to streams or storm drainages and that application timing should be considered for environmental protection.

  19. Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri

    SciTech Connect (OSTI)

    Cornish, Adam J.; Green, Robin; Gärtner, Katrin; Mason, Saundra; Hegg, Eric L.

    2015-04-30

    Hydrogen gas functions as a key component in the metabolism of a wide variety of microorganisms, often acting as either a fermentative end-product or an energy source. The number of organisms reported to utilize hydrogen continues to grow, contributing to and expanding our knowledge of biological hydrogen processes. Here we demonstrate that Volvox carteri f. nagariensis, a multicellular green alga with differentiated cells, evolves H2 both when supplied with an abiotic electron donor and under physiological conditions. The genome of Volvox carteri contains two genes encoding putative [FeFe]-hydrogenases (HYDA1 and HYDA2), and the transcripts for these genes accumulate under anaerobic conditions. The HYDA1 and HYDA2 gene products were cloned, expressed, and purified, and both are functional [FeFe]-hydrogenases. Additionally, within the genome the HYDA1 and HYDA2 genes cluster with two putative genes which encode hydrogenase maturation proteins. This gene cluster resembles operon-like structures found within bacterial genomes and may provide further insight into evolutionary relationships between bacterial and algal [FeFe]-hydrogenase genes.

  20. Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cornish, Adam J.; Green, Robin; Gärtner, Katrin; Mason, Saundra; Hegg, Eric L.

    2015-04-30

    Hydrogen gas functions as a key component in the metabolism of a wide variety of microorganisms, often acting as either a fermentative end-product or an energy source. The number of organisms reported to utilize hydrogen continues to grow, contributing to and expanding our knowledge of biological hydrogen processes. Here we demonstrate that Volvox carteri f. nagariensis, a multicellular green alga with differentiated cells, evolves H2 both when supplied with an abiotic electron donor and under physiological conditions. The genome of Volvox carteri contains two genes encoding putative [FeFe]-hydrogenases (HYDA1 and HYDA2), and the transcripts for these genes accumulate under anaerobicmore » conditions. The HYDA1 and HYDA2 gene products were cloned, expressed, and purified, and both are functional [FeFe]-hydrogenases. Additionally, within the genome the HYDA1 and HYDA2 genes cluster with two putative genes which encode hydrogenase maturation proteins. This gene cluster resembles operon-like structures found within bacterial genomes and may provide further insight into evolutionary relationships between bacterial and algal [FeFe]-hydrogenase genes.« less

  1. Use of prolines for improving growth and other properties of plants and algae

    DOE Patents [OSTI]

    Unkefer, Pat J.; Knight, Thomas J.; Martinez, Rodolfo A.

    2004-12-14

    Increasing the concentration of prolines, such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that over-express glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

  2. Use of prolines for improving growth and other properties of plants and algae

    DOE Patents [OSTI]

    Unkefer, Pat J.; Knight, Thomas J.; Martinez, Rodolfo A.

    2003-07-15

    Increasing the concentration of prolines, such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that over-express glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

  3. Use of prolines for improving growth and other properties of plants and algae

    DOE Patents [OSTI]

    Unkefer, Pat J.; Knight, Thomas J.; Martinez, Rodolfo A.

    2003-04-29

    Increasing the concentration of prolines such as 2-hydroxy-5-oxoproline, in the foliar portions of plants has been shown to cause an increase in carbon dioxide fixation, growth rate, dry weight, nutritional value (amino acids), nodulation and nitrogen fixation, photosynthetically derived chemical energy, and resistance to insect pests over the same properties for wild type plants. This can be accomplished in four ways: (1) the application of a solution of the proline directly to the foliar portions of the plant by spraying these portions; (2) applying a solution of the proline to the plant roots; (3) genetically engineering the plant and screening to produce lines that overexpress glutamine synthetase in the leaves which gives rise to increased concentration of the metabolite, 2-hydroxy-5-oxoproline (this proline is also known as 2-oxoglutaramnate); and (4) impairing the glutamine synthetase activity in the plant roots which causes increased glutamine synthetase activity in the leaves which gives rise to increased concentration of 2-hydroxy-5-oxoproline. Prolines have also been found to induce similar effects in algae.

  4. Molecular Breeding Algae For Improved Traits For The Conversion Of Waste To Fuels And Commodities.

    SciTech Connect (OSTI)

    Bagwell, C.

    2015-10-14

    This Exploratory LDRD aimed to develop molecular breeding methodology for biofuel algal strain improvement for applications in waste to energy / commodity conversion technologies. Genome shuffling technologies, specifically protoplast fusion, are readily available for the rapid production of genetic hybrids for trait improvement and have been used successfully in bacteria, yeast, plants and animals. However, genome fusion has not been developed for exploiting the remarkable untapped potential of eukaryotic microalgae for large scale integrated bio-conversion and upgrading of waste components to valued commodities, fuel and energy. The proposed molecular breeding technology is effectively sexual reproduction in algae; though compared to traditional breeding, the molecular route is rapid, high-throughput and permits selection / improvement of complex traits which cannot be accomplished by traditional genetics. Genome fusion technologies are the cutting edge of applied biotechnology. The goals of this Exploratory LDRD were to 1) establish reliable methodology for protoplast production among diverse microalgal strains, and 2) demonstrate genome fusion for hybrid strain production using a single gene encoded trait as a proof of the concept.

  5. Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Deng, Junjing; Vine, David J.; Chen, Si; Nashed, Youssef S. G.; Jin, Qiaoling; Phillips, Nicholas W.; Peterka, Tom; Ross, Rob; Vogt, Stefan; Jacobsen, Chris J.

    2015-02-24

    Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolutionmore » beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub–30-nm resolution structural images and ~90-nm–resolution fluorescence images of several elements in frozen-hydrated green algae. This combined approach offers a way to study the role of trace elements in their structural context.« less

  6. Characterization of the bacterial metagenome in an industrial algae bioenergy production system

    SciTech Connect (OSTI)

    Huang, Shi; Fulbright, Scott P; Zeng, Xiaowei; Yates, Tracy; Wardle, Greg; Chisholm, Stephen T; Xu, Jian; Lammers, Peter

    2011-03-16

    Cultivation of oleaginous microalgae for fuel generally requires growth of the intended species to the maximum extent supported by available light. The presence of undesired competitors, pathogens and grazers in cultivation systems will create competition for nitrate, phosphate, sulfate, iron and other micronutrients in the growth medium and potentially decrease microalgal triglyceride production by limiting microalgal health or cell density. Pathogenic bacteria may also directly impact the metabolism or survival of individual microalgal cells. Conversely, symbiotic bacteria that enhance microalgal growth may also be present in the system. Finally, the use of agricultural and municipal wastes as nutrient inputs for microalgal production systems may lead to the introduction and proliferation of human pathogens or interfere with the growth of bacteria with beneficial effects on system performance. These considerations underscore the need to understand bacterial community dynamics in microalgal production systems in order to assess microbiome effects on microalgal productivity and pathogen risks. Here we focus on the bacterial component of microalgal production systems and describe a pipeline for metagenomic characterization of bacterial diversity in industrial cultures of an oleaginous alga, Nannochloropsis salina. Environmental DNA was isolated from 12 marine algal cultures grown at Solix Biofuels, a region of the 16S rRNA gene was amplified by PCR, and 16S amplicons were sequenced using a 454 automated pyrosequencer. The approximately 70,000 sequences that passed quality control clustered into 53,950 unique sequences. The majority of sequences belonged to thirteen phyla. At the genus level, sequences from all samples represented 169 different genera. About 52.94% of all sequences could not be identified at the genus level and were classified at the next highest possible resolution level. Of all sequences, 79.92% corresponded to 169 genera and 70 other taxa. We apply a principal component analysis across the initial sample set to draw correlations between sample variables and changes in microbiome populations.

  7. Algae as a Feedstock for Biofuels: An Assessment of the State of Technology and Opportunities. Final Report

    SciTech Connect (OSTI)

    Sikes, K.; McGill, R.; Van Walwijk, M.

    2011-05-15

    The pursuit of a stable, economically-sound, and environmentally-friendly source of transportation fuel has led to extensive research and development (R&D) efforts focused on the conversion of various feedstocks into biofuels. Some feedstocks, such as sugar cane, corn and woody biomass, are targeted because their structures can be broken down into sugars and fermented into alcohols. Other feedstocks, such as vegetable oils, are appealing because they contain considerable amounts of lipids, which can be extracted and converted into biodiesel or other fuels. While significant R&D and commercial strides have been made with each of these feedstocks, technical and market barriers (e.g., cost, scalability, infrastructure requirements, and 'food vs. fuel' debates) currently limit the penetration of the resultant biofuels into the mainstream. Because of algae's ability to potentially address several of these barriers, its use as a feedstock for biofuels has led to much excitement and initiative within the energy industry. Algae are highly diverse, singleor multi-cellular organisms comprised of mostly lipids, protein, and carbohydrates, which may be used to produce a wide variety of biofuels. Algae offer many competitive advantages over other feedstocks, including: 1) Higher potential lipid content than terrestrial plants, sometimes exceeding 50% of the cell's dry biomass (U.S. DOE, May '10; Tornabene et al., 1983) 2) Rapid growth rates that are 20-30 times higher than terrestrial crops (McDill, 2009) and, in some cases, capable of doubling in size with 10 hours 3) Diverse number of species that can collectively thrive in a wide range of environments throughout the world, presenting an overall high overall tolerance for climate, sunlight, nutrient levels, etc. 4) Daily harvesting potential instead of seasonal harvest periods associated with terrestrial crops 5) Potential to redirect CO2 from industry operations to algal cultivation facilities to be used in an algal biofuel cycle before it is released into the atmosphere 6) Ability to be cultivated on land that that is unsuitable for agriculture, so it does not directly compete with farmland Given microalgae's high lipid content and rapid growth rates, maximum oil yields of 20,000--115,000 L/ha/yr (2,140-13,360 gal/ac/yr) have been estimated. xiv 7) Ability to thrive in seawater, wastewater, or other non-potable sources, so it does not directly compete with fresh water resources. In fact, wastewater can provide algae with some essential nutrients, such as nitrogen, so algae may contribute to cleaning up wastewater streams. 8) Non-toxic and biodegradable 9) Co-products that may present high value in other markets, including nutriceuticals and cosmetics Given microalgae's high lipid content and rapid growth rate, maximum oil yields of 20,000 -- 115,000 liters per hectare per year (L/ha/yr) (2,140 -- 13,360 gallons per acre per year) (Baldos, 2009; Wijffels, 2008) have been estimated, which is considerably higher than any other competing feedstock. Although algae species collectively present many strong advantages (although one specific species is unlikely to possess all of the advantages listed), a sustainable algal biofuel industry is at least one or two decades away from maturity, and no commercial scale operations currently exist. Several barriers must first be overcome before algal biofuels can compete with traditional petroleum-based fuels. Production chains with net energy output need to be identified, and continued R&D is needed to reduce the cost in all segments of the production spectrum (e.g., harvesting, dewatering, extracting of oil). Further research to identify strains with high production rates and/or oil yields may also improve competitiveness within the market. Initiatives to seamlessly integrate algal biofuels into the existing transportation infrastructure may increase their convenience level.

  8. The GC-Rich Mitochondrial and Plastid Genomes of the Green Alga Coccomyxa Give Insight into the Evolution of Organelle DNA Nucleotide Landscape

    SciTech Connect (OSTI)

    Smith, David Roy; Burki, Fabien; Yamada, Takashi; Grimwood, Jane; Grigoriev, Igor V.; Van Etten, James L.; Keeling, Patrick J.

    2011-05-13

    Most of the available mitochondrial and plastid genome sequences are biased towards adenine and thymine (AT) over guanine and cytosine (GC). Examples of GC-rich organelle DNAs are limited to a small but eclectic list of species, including certain green algae. Here, to gain insight in the evolution of organelle nucleotide landscape, we present the GC-rich mitochondrial and plastid DNAs from the trebouxiophyte green alga Coccomyxa sp. C-169. We compare these sequences with other GC-rich organelle DNAs and argue that the forces biasing them towards G and C are nonadaptive and linked to the metabolic and/or life history features of this species. The Coccomyxa organelle genomes are also used for phylogenetic analyses, which highlight the complexities in trying to resolve the interrelationships among the core chlorophyte green algae, but ultimately favour a sister relationship between the Ulvophyceae and Chlorophyceae, with the Trebouxiophyceae branching at the base of the chlorophyte crown.

  9. Fuel from wastewater : harnessing a potential energy source in Canada through the co-location of algae biofuel production to sources of effluent, heat and CO2.

    SciTech Connect (OSTI)

    Passell, Howard David; Whalen, Jake; Pienkos, Philip P.; O'Leary, Stephen J.; Roach, Jesse Dillon; Moreland, Barbara D.; Klise, Geoffrey Taylor

    2010-12-01

    Sandia National Laboratories is collaborating with the National Research Council (NRC) Canada and the National Renewable Energy Laboratory (NREL) to develop a decision-support model that will evaluate the tradeoffs associated with high-latitude algae biofuel production co-located with wastewater, CO2, and waste heat. This project helps Canada meet its goal of diversifying fuel sources with algae-based biofuels. The biofuel production will provide a wide range of benefits including wastewater treatment, CO2 reuse and reduction of demand for fossil-based fuels. The higher energy density in algae-based fuels gives them an advantage over crop-based biofuels as the 'production' footprint required is much less, resulting in less water consumed and little, if any conversion of agricultural land from food to fuel production. Besides being a potential source for liquid fuel, algae have the potential to be used to generate electricity through the burning of dried biomass, or anaerobically digested to generate methane for electricity production. Co-locating algae production with waste streams may be crucial for making algae an economically valuable fuel source, and will certainly improve its overall ecological sustainability. The modeling process will address these questions, and others that are important to the use of water for energy production: What are the locations where all resources are co-located, and what volumes of algal biomass and oil can be produced there? In locations where co-location does not occur, what resources should be transported, and how far, while maintaining economic viability? This work is being funded through the U.S. Department of Energy (DOE) Biomass Program Office of Energy Efficiency and Renewable Energy, and is part of a larger collaborative effort that includes sampling, strain isolation, strain characterization and cultivation being performed by the NREL and Canada's NRC. Results from the NREL / NRC collaboration including specific productivities of selected algal strains will eventually be incorporated into this model.

  10. Algal Testbed Public Private Partnerships Workshop on Principles and Processes: Algae Culture Management, Production and Downstream Harvesting

    Broader source: Energy.gov [DOE]

    The Spring 2016 ATP3 workshop will occur May 16th-20th at Santa Fe Community College (SFCC) and the Los Alamos National Lab's New Mexico Consortium (LANL NMC). These unique facilities will give participants incredible insights into aspects across the algae value chain and the food, energy and water nexus. Lectures will cover the fundamentals of managing microalgal cultures, culturing techniques, measuring and analyzing biomass, harvesting and processing technologies, as well as life cycle analysis and operations at the production scale. Participants will have opportunities to work in the laboratory and learn how to measure culture density (cell counting and optical density), use a light and fluorescence microscope, use flow cytometry, and perform gravimetric analyses (dry weight and ash-free dry weight), and techniques necessary to analyze biomass compounds.

  11. CX-100364 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Algae Production CO2 Absorber with Immobilized Carbonic Anhydrase Award Number: DE-EE0007092 CX(s) Applied: A9 Bioenergy Technologies Office Date: 09/08/2015 Location(s): CA Office(s): Golden Field Office

  12. CX-011696: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Algae Testbed Public-Private Partnership (ATP3) - a RAFT Partnership CX(s) Applied: A9, B3.6, B5.15 Date: 01/16/2014 Location(s): Arizona Offices(s): Golden Field Office

  13. CX-009895: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    25A1786 - Scaling and Commercialization of Algae Harvesting Technologies CX(s) Applied: B3.6 Date: 01/14/2010 Location(s): Ohio, Indiana, Alabama, California Offices(s): Advanced Research Projects Agency-Energy

  14. CX-100059 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Pilot-Scale Mixotrophic Algae Integrated Biorefinery Award Number: DE-EE0006245 CX(s) Applied: A9, B5.15 Date: 09/15/2014 Location(s): IA Office(s): Golden Field Office

  15. CX-010749: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Pilot-Scale Mixotrophic Algae Integrated Biorefinery CX(s) Applied: A9, B5.15 Date: 08/15/2013 Location(s): Illinois Offices(s): Golden Field Office

  16. Strain selection, biomass to biofuel conversion, and resource colocation have strong impacts on the economic performance of algae cultivation sites

    SciTech Connect (OSTI)

    Venteris, Erik R.; Wigmosta, Mark S.; Coleman, Andre M.; Skaggs, Richard

    2014-09-16

    Decisions involving strain selection, biomass to biofuel technology, and the location of cultivation facilities can strongly influence the economic viability of an algae-based biofuel enterprise. In this contribution we summarize our past results in a new analysis to explore the relative economic impact of these design choices. We present strain-specific growth model results from two saline strains (Nannocloropsis salina, Arthrospira sp.), a fresh to brackish strain (Chlorella sp., DOE strain 1412), and a freshwater strain of the order Sphaeropleales. Biomass to biofuel conversion is compared between lipid extraction (LE) and hydrothermal liquefaction (HTL) technologies. National-scale models of water, CO2 (as flue gas), land acquisition, site leveling, construction of connecting roads, and transport of HTL oil to existing refineries are used in conjunction with estimates of fuel value (from HTL) to prioritize and select from 88,692 unit farms (UF, 405 ha in pond area), a number sufficient to produce 136E+9 L yr-1 of renewable diesel (36 billion gallons yr-1, BGY). Strain selection and choice of conversion technology have large economic impacts, with differences between combinations of strains and biomass to biofuel technologies being up to $10 million dollars yr-1 UF-1. Results based on the most productive species, HTL-based fuel conversion, and resource costs show that the economic potential between geographic locations within the selection can differ by up to $4 million yr-1 UF-1, with 2.0 BGY of production possible from the most cost-effective sites. The local spatial variability in site rank is extreme, with very high and low rank sites within 10s of km of each other. Colocation with flue gas sources has a strong influence on site rank, but the most costly resource component varies from site to site. The highest rank sites are located predominantly in Florida and Texas, but most states south of 37°N latitude contain promising locations. Keywords: algae, biofuels, resource assessment, geographic information systems, techno-economics

  17. New Screening System Detects Algae with Increased H2 Production (Fact Sheet), NREL Highlights in Science, NREL (National Renewable Energy Laboratory)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL's high-throughput screen facilitates the selection of novel H 2 -producing algae. Researchers at the National Renewable Energy Laboratory (NREL) have developed a powerful method for screening through million-member algal libraries for strains with increased hydro- gen production. The screen uses H 2 -sensing bacteria that fluoresce when hydrogen is detected and is used as an agar overlay on top of growing algal colonies. The screen was first verified by comparing algal strains that

  18. CX-006439: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Novel Heterotrophic Algae ReactorCX(s) Applied: B3.6Date: 08/05/2011Location(s): CaliforniaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  19. Significant Increase in Hydrogen Photoproduction Rates and Yields by Wild-Type Algae is Detected at High Photobioreactor Gas Phase Volume (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-07-01

    This NREL Hydrogen and Fuel Cell Technical Highlight describes how hydrogen photoproduction activity in algal cultures can be improved dramatically by increasing the gas-phase to liquid-phase volume ratio of the photobioreactor. NREL, in partnership with subcontractors from the Institute of Basic Biological Problems in Pushchino, Russia, demonstrated that the hydrogen photoproduction rate in algal cultures always decreases exponentially with increasing hydrogen partial pressure above the culture. The inhibitory effect of high hydrogen concentrations in the photobioreactor gas phase on hydrogen photoproduction by algae is significant and comparable to the effect observed with some anaerobic bacteria.

  20. ATP3 Algae Testbed

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and Program Management Arizona State University March 25, 2015 Goal Statement Mission: Establish a sustainable network of regional testbeds that empowers knowledge creation and ...

  1. Algae Protein Fermentation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    hydrolysis and recovery of algal carbohydrates and lipids o FY14 LBL-ABPDU (10%): ... H 2 SO 4 ) pretreatment is effective for solubilizing >80% of the proteins & carbohydrates...

  2. Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*

    SciTech Connect (OSTI)

    Molnár, István; Lopez, David; Wisecaver, Jennifer H.; Devarenne, Timothy P.; Weiss, Taylor L.; Pellegrini, Matteo; Hackett, Jeremiah D.

    2012-10-30

    Microalgae hold promise for yielding a biofuel feedstock that is sustainable, carbon-neutral, distributed, and only minimally disruptive for the production of food and feed by traditional agriculture. Amongst oleaginous eukaryotic algae, the B race of Botryococcus braunii is unique in that it produces large amounts of liquid hydrocarbons of terpenoid origin. These are comparable to fossil crude oil, and are sequestered outside the cells in a communal extracellular polymeric matrix material. The biosynthetic engineering of terpenoid bio-crude production requires identification of genes and reconstruction of metabolic pathways responsible for production of both hydrocarbons and other metabolites of the alga that compete for photosynthetic carbon and energy.

  3. Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Molnár, István; Lopez, David; Wisecaver, Jennifer H.; Devarenne, Timothy P.; Weiss, Taylor L.; Pellegrini, Matteo; Hackett, Jeremiah D.

    2012-10-30

    Microalgae hold promise for yielding a biofuel feedstock that is sustainable, carbon-neutral, distributed, and only minimally disruptive for the production of food and feed by traditional agriculture. Amongst oleaginous eukaryotic algae, the B race of Botryococcus braunii is unique in that it produces large amounts of liquid hydrocarbons of terpenoid origin. These are comparable to fossil crude oil, and are sequestered outside the cells in a communal extracellular polymeric matrix material. The biosynthetic engineering of terpenoid bio-crude production requires identification of genes and reconstruction of metabolic pathways responsible for production of both hydrocarbons and other metabolites of the alga thatmore » compete for photosynthetic carbon and energy.« less

  4. Siting algae cultivation facilities for biofuel production in the United States: trade-offs between growth rate, site constructability, water availability, and infrastructure

    SciTech Connect (OSTI)

    Venteris, Erik R.; McBride, Robert; Coleman, Andre M.; Skaggs, Richard; Wigmosta, Mark S.

    2014-02-21

    Locating sites for new algae cultivation facilities is a complex task. The climate must support high growth rates, and cultivation ponds require appropriate land and water resources as well as key utility and transportation infrastructure. We employ our spatiotemporal Biomass Assessment Tool (BAT) to select promising locations based on the open-pond cultivation of Arthrospira sp. and a strain of the order Desmidiales. 64,000 potential sites across the southern United States were evaluated. We progressively apply a range of screening criteria and track their impact on the number of selected sites, geographic location, and biomass productivity. Both strains demonstrate maximum productivity along the Gulf of Mexico coast, with the highest values on the Florida peninsula. In contrast, sites meeting all selection criteria for Arthrospira were located along the southern coast of Texas and for Desmidiales were located in Louisiana and southern Arkansas. Site selection was driven mainly by the lack of oil pipeline access in Florida and elevated groundwater salinity in southern Texas. The requirement for low salinity freshwater (<400 mg L-1) constrained Desmidiales locations; siting flexibility is greater for salt-tolerant species such as Arthrospira. Combined siting factors can result in significant departures from regions of maximum productivity but are within the expected range of site-specific process improvements.

  5. Realization of Algae Potential Algae Biomass Yield Program

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... CO 2 delivery and energy consumption Achieve harvest density of 2-3 gL to lower harvesting costs Heat ... expandable from 4 to >75 linear feet 100 Lm 2 at 10 cm ...

  6. Mining fatty acids from algae

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Minimizing Energy Losses in Ducts Minimizing Energy Losses in Ducts Placing ductwork in conditioned space can help reduce energy losses. | Photo courtesy of ©iStockphoto/SimplyCreativePhotography Placing ductwork in conditioned space can help reduce energy losses. | Photo courtesy of ©iStockphoto/SimplyCreativePhotography In new home construction or in retrofits, proper duct system design is critical. In recent years, energy-saving designs have sought to include ducts and heating systems in

  7. Algae R&D Activities

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... with CHG Processing * Enable Nutrient Recycle Priority 2 Improve Extraction- Conversion ... Functionalized Mesoporous Silica Nanoparticles 9. LANL S121847 ...

  8. CX-100014: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Realization of Algae Potential CX(s) Applied: A9, B3.6, B5.15 Date: 08/19/2014 Location(s): New Mexico Offices(s): Golden Field Office Technology Office: Bioenergy Program Award Number: DE-EE0006313

  9. CX-009565: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Development of Bio-Oil Commodity Fuel as a Refinery Feedstock From High Impact Algae Biomass CX(s) Applied: A9, B3.6 Date: 12/12/2012 Location(s): Georgia Offices(s): Golden Field Office

  10. CX-010845: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Temporary Modification (ETP-TMC-13-01) to Install an Ultrasonic Sound Emitting Device to Control Algae in the H-Retention Basin CX(s) Applied: B3.6 Date: 07/31/2013 Location(s): South Carolina Offices(s): Savannah River Operations Office

  11. CX-011159: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Temporary Modification (ETP-TMC-13-01) to Install an Ultrasonic Sound-Emitting Device to Control Algae in the H-Retention Basin CX(s) Applied: B3.6 Date: 08/13/2013 Location(s): South Carolina Offices(s): Savannah River Operations Office

  12. CX-100500 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    PACE: Producing Algae for Coproducts and Energy Award Number: DE-EE0007089 CX(s) Applied: A9, B3.6 Bioenergy Technologies Office Date: 02/25/2016 Location(s): CO Office(s): Golden Field Office

  13. CX-100518 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    PACE: Producing Algae for Coproducts and Energy Award Number: DE-EE0007089 CX(s) Applied: A9, B3.6 Bioenergy Technologies Office Date: 02/25/2016 Location(s): CO Office(s): Golden Field Office

  14. CX-100573 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Marine AlGae Industrialization Consortium (MAGIC): Combining biofuel and high-value bioproducts to meet the RFS Award Number: DE-EE0007091 CX(s) Applied: A9, B3.6, B5.15 Bioenergy Technologies Office Date: 03/18/2016 Location(s): NC Office(s): Golden Field Office

  15. CX-100363 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Marine Algae Industrialization Consortium (MAGIC): Combining biofuel and high-value bioproducts to meet the RFS Award Number: DE-EE0007091 CX(s) Applied: A9 Bioenergy Technologies Office Date: 09/08/2015 Location(s): NC Office(s): Golden Field Office

  16. CX-100341 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    PACE: Producing Algae for Co-products and Energy Award Number: DE-EE0007089 CX(s) Applied: A9, B3.6 Bioenergy Technologies Office Date: 08/31/2015 Location(s): CO Office(s): Golden Field Office

  17. CX-100482 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Algae Testbed Public-Private Partnership (ATP3) – a RAFT Partnership Award Number: DE-EE0005996 CX(s) Applied: A9, B3.6, B5.15 Bioenergy Technology Office Date: 01/16/2014 Location(s): AZ Office(s): Golden Field Office

  18. CX-100111 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Hydrothermal Liquefaction Pathways for Low-Nitrogen Biocrude from Wet Algae Award Number: DE-EE0006635 CX(s) Applied: A9, B3.16, B3.6 Date: 10/29/2014 Location(s): CA Office(s): Golden Field Office

  19. Characterization of the Kootenai River Algae Community and Primary Productivity Before and After Experimental Nutrient Addition, 2004–2007 [Chapter 2, Kootenai River Algal Community Characterization, 2009 KTOI REPORT].

    SciTech Connect (OSTI)

    Holderman, Charlie; Anders, Paul; Shafii, Bahman

    2009-07-01

    The Kootenai River ecosystem (spelled Kootenay in Canada) has experienced numerous ecological changes since the early 1900s. Some of the largest impacts to habitat, biological communities, and ecological function resulted from levee construction along the 120 km of river upstream from Kootenay Lake, completed by the 1950s, and the construction and operation of Libby Dam on the river near Libby Montana, completed in 1972. Levee construction isolated tens of thousands of hectares of historic functioning floodplain habitat from the river channel downstream in Idaho and British Columbia (B.C.) severely reducing natural biological productivity and habitat diversity crucial to large river-floodplain ecosystem function. Libby Dam greatly reduces sediment and nutrient transport to downstream river reaches, and dam operations cause large changes in the timing, duration, and magnitude of river flows. These and other changes have contributed to the ecological collapse of the post-development Kootenai River ecosystem and its native biological communities. In response to large scale loss of nutrients, experimental nutrient addition was initiated in the North Arm of Kootenay Lake in 1992, in the South Arm of Kootenay Lake in 2004, and in the Kootenai River at the Idaho-Montana border during 2005. This report characterizes baseline chlorophyll concentration and accrual (primary productivity) rates and diatom and algal community composition and ecological metrics in the Kootenai River for four years, one (2004) before, and three (2005 through 2007) after nutrient addition. The study area encompassed a 325 km river reach from the upper Kootenay River at Wardner, B.C. (river kilometer (rkm) 445) downstream through Montana and Idaho to Kootenay Lake in B.C. (rkm 120). Sampling reaches included an unimpounded reach furthest upstream and four reaches downstream from Libby Dam affected by impoundment: two in the canyon reach (one with and one without nutrient addition), a braided reach, and a meandering reach. The study design included 14 sampling sites: an upstream, unimpounded reference site (KR-14), four control (non-fertilized) canyon sites downstream from Libby Dam, but upstream from nutrient addition (KR-10 through KR-13), two treatment sites referred to collectively as the nutrient addition zone (KR-9 and KR-9.1, located at and 5 km downstream from the nutrient addition site), two braided reach sites (KR-6 and KR-7), and four meander reach sites (KR-1 through KR-4). A series of qualitative evaluations and quantitative analyses were used to assess baseline conditions and effects of experimental nutrient addition treatments on chlorophyll, primary productivity, and taxonomic composition and metric arrays for the diatom and green algae communities. Insufficient density in the samples precluded analyses of bluegreen algae taxa and metrics for pre- and post-nutrient addition periods. Chlorophyll a concentration (mg/m{sup 2}), chlorophyll accrual rate (mg/m{sup 2}/30d), total chlorophyll concentration (chlorophyll a and b) (mg/m{sup 2}), and total chlorophyll accrual rate (mg/m{sup 2}/30d) were calculated. Algal taxa were identified and grouped by taxonomic order as Cyanophyta (blue-greens), Chlorophyta (greens), Bacillariophyta (diatoms), Chrysophyta (goldens), and dominant species from each sample site were identified. Algal densities (number/ml) in periphyton samples were calculated for each sample site and sampling date. Principal Component Analysis (PCA) was performed to reduce the dimension of diatom and algae data and to determine which taxonomic groups and metrics were contributing significantly to the observed variation. PCA analyses were tabulated to indicate eigenvalues, proportion, and cumulative percent variation, as well as eigenvectors (loadings) for each of the components. Biplot graphic displays of PCA axes were also generated to characterize the pattern and structure of the underlying variation. Taxonomic data and a series of biological and ecological metrics were used with PCA for diatoms and algae. Algal metrics included a suite of abundance, diversity, richness, dominance, and other measures, whereas additional trophic status and chemical limnology metrics, Van Dam indices and morphological groupings were employed in diatom PCAs. Analysis of Variance (ANOVA) was carried out using chlorophyll metrics and taxa and metric arrays for the diatom and green algae community data for comparing site differences from 2004 through 2007. Clear, statistically significant, biological responses from chlorophyll metrics, and taxa and metrics of the diatom and algal communities were revealed following experimental nutrient addition in the Kootenai River. Chlorophyll metric responses were more often significant and generally greater in magnitude than diatom and green algae taxa and metric responses.

  20. Solazyme Pilot-Scale Biorefinery | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solazyme Pilot-Scale Biorefinery Solazyme Pilot-Scale Biorefinery The Solazyme integrated biorefinery will use a heterotrophic algal oil biomanufacturing process to create biofuels. PDF icon ibr_arra_solazyme.pdf More Documents & Publications CX-005693: Categorical Exclusion Determination Algae Biofuels Technology 2013 Peer Review Presnentations-Plenaries

  1. Whole Algae Hydrothermal Liquefaction Technology Pathway

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... (2010), Duan and Savage (2011), Biller and Ross (2011), Jena et al. (2011), Valdez et al. ... Energy & Fuels (26); pp. 642-657. Biller, P.; Ross, A. (2011). "Potential Yields and ...

  2. Whole Algae Hydrothermal Liquefaction Technology Pathway

    SciTech Connect (OSTI)

    Biddy, M.; Davis, R.; Jones, S.

    2013-03-01

    This technology pathway case investigates the feasibility of using whole wet microalgae as a feedstock for conversion via hydrothermal liquefaction. Technical barriers and key research needs have been assessed in order for the hydrothermal liquefaction of microalgae to be competitive with petroleum-derived gasoline-, diesel-, and jet-range hydrocarbon blendstocks.

  3. Rewiring Algae's Catalytic Circuits - Continuum Magazine | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Revolution...Now The Future Arrives for Five Clean Energy Technologies - 2015 Update November 2015 ii Contributors Luke Bassett, DOE Office of Energy Policy & Systems Analysis and Office of Energy Efficiency & Renewable Energy James Brodrick, DOE Building Technologies Office Steve Capanna, DOE Office of Energy Efficiency & Renewable Energy Jonathan Castellano, DOE Vehicle Technologies Office Christy Cooper, DOE Vehicle Technologies Office Paul Donohoo-Vallett, DOE Office of Energy

  4. Carbon2Algae, LLC | Open Energy Information

    Open Energy Info (EERE)

    facilities through the utilization of captured CO2 emissions to produce high quality bio-fuel in all climatic conditions. This article is a stub. You can help OpenEI by...

  5. 2016 Bioenergizeme Infographic Challenge: Algae Biofuel

    Office of Energy Efficiency and Renewable Energy (EERE)

    This infographic was created by students from Sparks High School in Sparks, NV, as part of the U.S. Department of Energy-BioenergizeME Infographic Challenge. The BioenergizeME Infographic Challenge...

  6. 2016 Bioenergizeme Infographic Challenge: Space Algae

    Broader source: Energy.gov [DOE]

    This infographic was created by students from Cascade High School in Everett, WA, as part of the U.S. Department of Energy-BioenergizeME Infographic Challenge. The BioenergizeME Infographic...

  7. Whole Algae Hydrothermal Liquefaction Technology Pathway

    SciTech Connect (OSTI)

    Biddy, Mary J.; Davis, Ryan; Jones, Susanne B.; Zhu, Yunhua

    2013-03-31

    In support of the Bioenergy Technologies Office, the National Renewable Energy Laboratory (NREL) and the Pacific Northwest National Laboratory (PNNL) are undertaking studies of biomass conversion technologies to hydrocarbon fuels to identify barriers and target research toward reducing conversion costs. Process designs and preliminary economic estimates for each of these pathway cases were developed using rigorous modeling tools (Aspen Plus and Chemcad). These analyses incorporated the best information available at the time of development, including data from recent pilot and bench-scale demonstrations, collaborative industrial and academic partners, and published literature and patents. This pathway case investigates the feasibility of using whole wet microalgae as a feedstock for conversion via hydrothermal liquefaction. Technical barriers and key research needs have been assessed in order for the hydrothermal liquefaction of microalgae to be competitive with petroleum-derived gasoline, diesel and jet range blendstocks.

  8. Whole Algae Hydrothermal Liquefaction Technology Pathway Biddy...

    Office of Scientific and Technical Information (OSTI)

    MICROALGAL-DERIVED BIOFUEL; HYDROCARBON FUEL; BIOMASS TECHNOLOGIES OFFICE; NATIONAL RENEWABLE ENERGY LABORATORY; PACIFIC NORTHWEST NATIONAL LABORATORY; Bioenergy MICROALGAE;...

  9. Sustainable Development of Algae for Biofuel

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    level Project Overview Objectives and History (2.5 of 3 tasks are new) * Task 1. ... indicators and targets and moving toward best management practices - New subtask ...

  10. Wastewater Reclamation and Biofuel Production Using Algae

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Africa, New Zealand (but not designed for nutrient removal). 9 Typical Electro-Mechanical Treatment Plant 10 Aeration Basins with Air Blowers Sludge Settling Tanks -100,000 0...

  11. Heterotrophic Archaea Contribute to Carbon Cycling in Low-pH, Suboxic Biofilm Communities

    SciTech Connect (OSTI)

    Justice, Nicholas B; Pan, Chongle; Mueller, Ryan; Spaulding, Susan E.; Shah, Vega; Sun, Christine; Yelton, Alexis P; Miller, CS; Thomas, BC; Shah, Manesh B; Verberkmoes, Nathan C; Hettich, Robert {Bob} L; Banfield, Jillian F.

    2012-01-01

    Archaea are widely distributed and yet are most often not the most abundant members of microbial communities. Here, we document a transition from Bacteria- to Archaea-dominated communities in microbial biofilms sampled from the Richmond Mine acid mine drainage (AMD) system (pH 1.0,38 C) and in laboratory-cultivated biofilms. This transition occurs when chemoautotrophic microbial communities that develop at the air-solution interface sink to the sediment-solution interface and degrade under microaerobic and anaerobic conditions. The archaea identified in these sunken biofilms are from the class Thermoplasmata, and in some cases, the highly divergent ARMAN nanoarchaeal lineage. In several of the sunken biofilms, nanoarchaea comprise 10 to 25% of the community, based on fluorescent in situ hybridization and metagenomic analyses. Comparative community proteomic analyses show a persistence of bacterial proteins in sunken biofilms, but there is clear evidence for amino acid modifications due to acid hydrolysis. Given the low representation of bacterial cells in sunken biofilms based on microscopy, we infer that hydrolysis reflects proteins derived from lysed cells. For archaea, we detected 2,400 distinct proteins, including a subset involved in proteolysis and peptide uptake. Laboratory cultivation experiments using complex carbon substrates demonstrated anaerobic enrichment of Ferroplasma and Aplasma coupled to the reduction of ferric iron. These findings indicate dominance of acidophilic archaea in degrading biofilms and suggest that they play roles in anaerobic nutrient cycling at low pH.

  12. Final Report - Cycling of DOC and DON by novel heterotrophic and photoheterotrophic bacteria in the ocean

    SciTech Connect (OSTI)

    Royer, David F

    2011-06-10

    This report describes a collaboration between Lincoln University and the College of Earth, Ocean and Environment at the University of Delaware and was funded under the Department of Energy Biological Investigations – Ocean Margins Program (BI-OMP). The principal outcomes of the grant are (1) the opportunity for Lincoln students to participate in marine research at the University of Delaware, (2) the opportunity for participating students to present their research at a variety of scientific meetings, (3) the establishment of an environmental science major and a microbial ecology course at Lincoln, (4) the upgrade of research capabilities at Lincoln, and (5) the success of participating students in graduate and professional school.

  13. Cycling of DOC and DON by Novel Heterotrophic and Photoheterotrophic Bacteria in the Ocean: Final Report

    SciTech Connect (OSTI)

    Kirchman, David L

    2008-12-09

    The flux of dissolved organic matter (DOM) through aquatic bacterial communities is a major process in carbon cycling in the oceans and other aquatic systems. Our work addressed the general hypothesis that the phylogenetic make-up of bacterial communities and the abundances of key types of bacteria are important factors influencing the processing of DOM in aquatic ecosystems. Since most bacteria are not easily cultivated, the phylogenetic diversity of these microbes has to be assessed using culture-independent approaches. Even if the relevant bacteria were cultivated, their activity in the lab would likely differ from that under environmental conditions. This project found variation in DOM uptake by the major bacterial groups found in coastal waters. In brief, the data suggest substantial differences among groups in the use of high and molecular weight DOM components. It also made key discoveries about the role of light in affecting this uptake especially by cyanobacteria. In the North Atlantic Ocean, for example, over half of the light-stimulated uptake was by the coccoid cyanobacterium, Prochlorococcus, with the remaining uptake due to Synechococcus and other photoheterotrophic bacteria. The project also examined in detail the degradation of one organic matter component, chitin, which is often said to be the second most abundant compound in the biosphere. The findings of this project contribute to our understanding of DOM fluxes and microbial dynamics supported by those fluxes. It is possible that these findings will lead to improvements in models of the carbon cycle that have compartments for dissolved organic carbon (DOC), the largest pool of organic carbon in the oceans.

  14. NREL Researcher Discusses Revitalized Algae Program - News Releases...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Presentation on July 7 at University of Minnesota Available Online July 1, 2010 Dr. Philip ... research on July 7, from 3-5 p.m., at the University of Minnesota Twin Cities Campus. ...

  15. Improved Algae-based Biorefining and High-throughput Screening...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biomass and Biofuels Biomass and Biofuels Find More Like This Return to Search Improved ... culture is needed, and also that fluid transport limits shear stress on the cells, thus ...

  16. Multi-Scale Characterization of Improved Algae Strains

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Three Objectives 1. Establish a pipeline for evaluating improved strains under ... Improvements in the model should permit better predictions and testing of outdoor ...

  17. Sandia Algae Researchers Cut Costs with Improved Nutrient Recycling...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sandia molecular biologists Todd Lane and Ryan Davis, ... sustainable design by reducing the demand for ... Renewable Energy Laboratory researcher Lee Elliott ...

  18. Potential consequences of GM algae escape on ecosystem services

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Richard Sayre Los Alamos National Laboratory New Mexico Consortium Outline * Historical ... Renewable fuels and green chemical feedstocks 50-90% Other biomass 4-50% Oils Rapid growth ...

  19. Algae Testbed Public Private Partnership Workshop on Principles...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    laboratory and learn how to measure culture density (cell counting and optical density), use a ... weight and ash-free dry weight) and techniques necessary to analyze biomass ...

  20. 2016 Bioenergizeme Infographic Challenge: The Algae's Always Greener

    Broader source: Energy.gov [DOE]

    This infographic was created by students from Smithtown High School East in St. James, NY, as part of the U.S. Department of Energy-BioenergizeME Infographic Challenge. The BioenergizeME...

  1. DOE Announces Webinars on Genetically Modified Algae, NREL's...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Webinars on Fuel Cells at NASCAR, an Advanced Energy Retrofit Guide for Healthcare Facilities, and More DOE Announces Webinars on Top Solar Tools, Building Energy Optimization ...

  2. Whole Algae Hydrothermal Liquefaction: 2014 State of Technology

    SciTech Connect (OSTI)

    Jones, Susanne B.; Zhu, Yunhua; Snowden-Swan, Lesley J.; Anderson, Daniel; Hallen, Richard T.; Schmidt, Andrew J.; Albrecht, Karl O.; Elliott, Douglas C.

    2014-07-30

    This report describes the base case yields and operating conditions for converting whole microalgae via hydrothermal liquefaction and upgrading to liquid fuels. This serves as the basis against which future technical improvements will be measured.

  3. Buoyant triacylglycerol-filled green algae and methods therefor

    DOE Patents [OSTI]

    Goodenough, Ursula; Goodson, Carrie

    2015-04-14

    Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.

  4. Energy 101 | Algae-to-Fuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Erin R. Pierce Erin R. Pierce Former Digital Communications Specialist, Office of Public ... In this edition of our Energy 101 video series, we're taking a behind-the-scenes look at ...

  5. Sandia Energy - The National Algae Testbed Public-Private Partnership...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (ATP3) kick-off meeting at Arizona State University. Sandians Ron Pate (Earth Systems Analysis Dept.), Todd Lane (Systems Biology Dept.), Tricia Gharagozloo (ThermalFluid...

  6. Algae-to-Fuel: Integrating Thermochemical Conversion, Nutrient...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    silicon air battery development (DARPA) * Alane (AlH 3 ), hydrogen storage for fuel cells (DOE) * Low energy nuclear reaction (commercial client) * Selective separation...

  7. BETO Deputy Director Publishes Commentary on Development of Algae...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Valerie Sarisky-Reed's commentary, "Algal Progress Report," was published in the February edition of the bimonthly research journal Industrial Biotechnology. Her commentary details ...

  8. Enzyme Fusions Optimize Photosynthetic Hydrogen Production in Algae (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-04-01

    Research at NREL is demonstrating that engineering enzymes has the potential to improve efficiencies.

  9. Solazyme Developing Cheaper Algae Biofuels, Brings Jobs to Pennsylvani...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... The fermentation facility that once made food additives, however, was left idle, says Betse Humphrey, a spokesperson for Cherokee. In an effort to utilize the facility and workers, ...

  10. June 2012 News Blast: Algae on the Mind

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    June 2012 Biomass 2012 Is Less Than Two Weeks Away It's not too late to reserve your spot at Biomass 2012: Confronting Challenges, Creating Opportunities - Sustaining a Commitment to Bioenergy, the Energy Department's fifth annual networking and informational conference. Join Secretary of Energy Steven Chu, White House Energy Advisor Heather Zichal, members of Congress, and an exciting list of confirmed speakers by registering now! Visit the Biomass 2012 website to find out more information

  11. The Algae Foundation Announces New DOE Funded Education Initiative...

    Broader source: Energy.gov (indexed) [DOE]

    development and education 2015 Science Undergraduate Laboratory Internships spring interns and mentors at Lawrence Berkeley National Laboratory. Workforce development and education...

  12. 2016 Bioenergizeme Infographic Challenge: Algae as a Biofuel

    Office of Energy Efficiency and Renewable Energy (EERE)

    This infographic was created by students from Smithtown High School East in St. James, NY, as part of the U.S. Department of Energy-BioenergizeME Infographic Challenge. The BioenergizeME...

  13. Crow Nation Students Participate in Algae Biomass Research Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This article was originally published in the DOE Office of Indian Energy's Indian Energy Beat newsletter, which highlights opportunities and success stories in tribal energy ...

  14. Whole Turf Algae to biofuels-final-sm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    alternative for achieving higher and more reliable biofuel productivity at reduced costs. ... Utilizing a pulsed, thin turbulent flow across the field, biofuel feedstock is produced at ...

  15. Magneto-optical properties of biogenic photonic crystals in algae

    SciTech Connect (OSTI)

    Iwasaka, M.; Mizukawa, Y.

    2014-05-07

    In the present study, the effects of strong static magnetic fields on the structural colors of the cell covering crystals on a microalgae, coccolithophore, were investigated. The coccolithophore, Emiliania huxleyi, generates a precise assembly of calcite crystals called coccoliths by biomineralization. The coccoliths attached to the cells exhibited structural colors under side light illumination, and the colors underwent dynamic transitions when the magnetic fields were changed between 0?T and 5?T, probably due to diamagnetically induced changes of their inclination under the magnetic fields. The specific light-scattering property of individual coccoliths separated from the cells was also observed. Light scattering from a condensed suspension of coccoliths drastically decreased when magnetic fields of more than 4?T were applied parallel to the direction of observation. The magnetically aligned cell-covering crystals of the coccolithophores exhibited the properties of both a photonic crystal and a minimum micromirror.

  16. Cultivation of macroscopic marine algae and fresh water aquatic weeds

    SciTech Connect (OSTI)

    Ryther, J.H.

    1982-02-01

    The ORCA clone of the red seaweed Gracilaria tikvahiae has been in culture continuously for over two years. Yield for the past year has averaged 12 g ash-free dry wt/m/sup 2/ .day (17.5 t/a.y) in suspended 2600-1 aluminum tank cultures with four exchanges of enriched seawater per day and continuous aeration. Yields from nonintensive pond-bottom culture, similar to commercial Gracilaria culture methods in Taiwan, averaged 3 g afdw/m/sup 2/.day in preliminary experiments. Rope and spray cultures were not successful. Yields of water hyacinths from March 1978 to March 1979 averaged 25 g afdw/m/sup 2/.day (37 t/a.y). Season, nutrient availability (form and quantity) and stand density were found to affect the relative proportions of structural and nonstructural tissue in water hyacinths and thereby significantly affect digestibility of and methane production by the plants. Pennywort (Hydrocotyle) grew poorly in winter and its annual yield averaged only one-third that of water hyacinth. Water lettuce (Pistia) appears more comparable to hyacinths in preliminary studies and its yields will be monitored throughout a complete year. Stable, continuous anaerobic digestion of both water hyacinths and Gracilaria has been maintained with an average gas production from both species of 0.4 1/g volatile solids at 60% methane.

  17. Magnetic mesoporous material for the sequestration of algae

    DOE Patents [OSTI]

    Trewyn, Brian G.; Kandel, Kapil; Slowing, Igor Ivan; Lee, Show-Ling

    2014-09-09

    The present invention provides a magnetic mesoporous nanoparticle that includes a mesoporous silicate nanoparticle and iron oxide. The present invention also provides a method of using magnetic mesoporous nanoparticles to sequester microorganisms from a media.

  18. PetroAlgae formerly Dover Glen Inc | Open Energy Information

    Open Energy Info (EERE)

    University, and bred selectively over many generations, to produce rapid growth and high oil yield for biodiesel production. Coordinates: -37.817532, 144.967148 Show Map...

  19. Real Time Diagnostics for Algae-final-sm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Real-time Monitoring And Diagnostics Detecting pathogens and predators to quickly recover ... Real-time Monitoring With Online Algal Reflectance Monitor System Researchers have ...

  20. Energy 101: Algae-to-Fuel | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    sequence of cars, trains, and planes in motion. Extract that oil, and you have the raw materials to make fuel for cars, trucks, trains, and planes. In the future, anything that...

  1. EERE Success Story-California: Breakthrough in Algae Biology...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Using transcriptomics to guide identification of target lipases in the Thalassiosira pseudonana diatom, the team was able to demonstrate a genetics technique that could have ...

  2. Heterotrophic Soil Respiration in Warming Experiments: Using Microbial Indicators to Partition Contributions from Labile and Recalcitrant Soil Organic Carbon. Final Report

    SciTech Connect (OSTI)

    Bradford, M A; Melillo, J M; Reynolds, J F; Treseder, K K; Wallenstein, M D

    2010-06-10

    The central objective of the proposed work was to develop a genomic approach (nucleic acid-based) that elucidates the mechanistic basis for the observed impacts of experimental soil warming on forest soil respiration. The need to understand the mechanistic basis arises from the importance of such information for developing effective adaptation strategies for dealing with projected climate change. Specifically, robust predictions of future climate will permit the tailoring of the most effective adaptation efforts. And one of the greatest uncertainties in current global climate models is whether there will be a net loss of carbon from soils to the atmosphere as climate warms. Given that soils contain approximately 2.5 times as much carbon as the atmosphere, a net loss could lead to runaway climate warming. Indeed, most ecosystem models predict that climate warming will stimulate microbial decomposition of soil carbon, producing such a positive feedback to rising global temperatures. Yet the IPCC highlights the uncertainty regarding this projected feedback. The uncertainty arises because although warming-experiments document an initial increase in the loss of carbon from soils, the increase in respiration is short-lived, declining to control levels in a few years. This attenuation could result from changes in microbial physiology with temperature. We explored possible microbial responses to warming using experiments and modeling. Our work advances our understanding of how soil microbial communities and their activities are structured, generating insight into how soil carbon might respond to warming. We show the importance of resource partitioning in structuring microbial communities. Specifically, we quantified the relative abundance of fungal taxa that proliferated following the addition of organic substrates to soil. We added glycine, sucrose, cellulose, lignin, or tannin-protein to soils in conjunction with 3-bromo-deoxyuridine (BrdU), a nucleotide analog. Active microbes absorb BrdU from the soil solution; if they multiply in response to substrate additions, they incorporate the BrdU into their DNA. After allowing soils to incubate, we extracted BrdU-labeled DNA and sequenced the ITS regions of fungal rDNA. Fungal taxa that proliferated following substrate addition were likely using the substrate as a resource for growth. We found that the structure of active fungal communities varied significantly among substrates. The active fungal community under glycine was significantly different from those under other conditions, while the active communities under sucrose and cellulose were marginally different from each other and the control. These results indicate that the overall community structure of active fungi was altered by the addition of glycine, sucrose, and cellulose and implies that some fungal taxa respond to changes in resource availability. The community composition of active fungi is also altered by experimental warming. We found that glycine-users tended to increase under warming, while lignin-, tannin/protein-, and sucrose-users declined. The latter group of substrates requires extracellular enzymes for use, but glycine does not. It is possible that warming selects for fungal species that target, in particular, labile substrates. Linking these changes in microbial communities and resource partitioning to soil carbon dynamics, we find that substrate mineralization rates are, in general, significantly lower in soils exposed to long-term warming. This suggests that microbial use of organic substrates is impaired by warming. Yet effects are dependent on substrate identity. There are fundamental differences in the metabolic capabilities of the communities in the control and warmed soils. These differences might relate to the changes in microbial community composition, which appeared to be associated with groups specialized on different resources. We also find that functional responses indicate temperature acclimation of the microbial community. There are distinct seasonal patterns and to long-term soil warming, with higher-temperature optima for soils exposed to warmer temperatures. To relate these changes within the microbial community to potential positive feedbacks between climate warming and soil respiration, we develop a microbial-enzyme model to simulate the responses of soil carbon to warming. We find that declines in microbial biomass and degradative enzymes can explain the observed attenuation of soil-carbon emissions in response to warming. Specifically, reduced carbon-use efficiency limits the biomass of microbial decomposers and mitigates loss of soil carbon. However, microbial adaptation or a change in microbial communities could lead to an upward adjustment of the efficiency of carbon use, counteracting the decline in microbial biomass and accelerating soil-carbon loss. We conclude that the soil-carbon response to climate warming depends on the efficiency of soil microbes in using carbon.

  3. Recovery Act: Beneficial CO{sub 2} Capture in an Integrated Algal Biorefinery for Renewable Generation and Transportation Fuels

    SciTech Connect (OSTI)

    Lane, Christopher; Hampel, Kristin; Rismani-Yazdi, Hamid; Kessler, Ben; Moats, Kenneth; Park, Jonathan; Schwenk, Jacob; White, Nicholas; Bakhit, Anis; Bargiel, Jeff; Allnutt, F.C.

    2014-03-31

    DOE DE-FE0001888 Award, Phase 2, funded research, development, and deployment (RD&D) of Phycal’s pilot-scale, algae to biofuels, bioproducts, and processing facility in Hawai’i. Phycal’s algal-biofuel and bioproducts production system integrates several novel and mature technologies into a system that captures and reuses industrially produced carbon dioxide emissions, which would otherwise go directly to the atmosphere, for the manufacture of renewable energy products and bioproducts from algae (note that these algae are not genetically engineered). At the end of Phase 2, the project as proposed was to encompass 34 acres in Central Oahu and provide large open ponds for algal mass culturing, heterotrophic reactors for the Heteroboost™ process, processing facilities, water recycling facilities, anaerobic digestion facilities, and other integrated processes. The Phase 2 award was divided into two modules, Modules 1 & 2, where the Module 1 effort addressed critical scaling issues, tested highest risk technologies, and set the overall infrastructure needed for a Module 2. Phycal terminated the project prior to executing construction of the first Module. This Final Report covers the development research, detailed design, and the proposed operating strategy for Module 1 of Phase 2.

  4. Dynamics of radionuclide exchange in the calcareous algae Halimeda at Enewetak Atoll

    SciTech Connect (OSTI)

    Spies, R.B.; Marsh, K.V.; Kercher, J.R.

    1981-01-01

    Measurements of /sup 239 +240/Pu in the detrital inclusions and in acid-soluble and acid-insoluble fractions of Halimeda macrophysa showed a 10-fold higher concentration in the acid-insoluble coenocytic filaments than in the acid-soluble fraction. In a depuration experiment with Halimeda incrassata at Enewetak Atoll the loss rate of six radionuclides was measured. Data for /sup 60/Co, /sup 137/Cs, and /sup 102//sup m/Rh were fit to loss curves by using one term for exponential loss; data for /sup 155/Eu, /sup 239 +240/Pu, and /sup 241/Am required two terms. For each radionuclide, compartment size and transfer functions were determined for the apropriate one- and two-compartment models. Of 26 possible two-compartment models, only seven gave solutions with our data. Nearly identical loss rates were obtained for /sup 155/Eu, /sup 239 +240/Pu, and /sup 241/Am in the fast-exchanging compartments for all seven models. The uptake rates for these nuclides were also similar when uptake rates were normalized to local sediment concentrations. The fast-exchanging compartment probably corresponds to the mucilage surface layer of the coenocytic filaments. The identity of the slow-exchanging compartment is less certain but it may correspond to the skeletal surface.

  5. Dynamics of radionuclide exchange in the calcareous algae Halimeda at Enewetak Atoll

    SciTech Connect (OSTI)

    Spies, R.B.; Marsh, K.V.; Kercher, J.R.

    1981-01-01

    Measurements of /sup 239+240/Pu in the detrital inclusions and in acid-soluble and acid-insoluble fractions of Halimeda macrophysa showed a 10-fold higher concentration in the acid-insoluble coenocytic filaments than in the acid-soluble fraction. In a depuration experiment with Halimeda incrassata at Enewetak Atoll the loss rate of six radionuclides was measured. Data for /sup 60/Co, /sup 137/Cs, and /sup 102m/Rh were fit to loss curves by using one term for exponential loss; data for /sup 155/Eu, /sup 239+240/Pu, and /sup 241/Am required two terms. For each radionuclide, compartment size and transfer functions were determined for the appropriate one- and two-compartment models. Of 26 possible two-compartment models, only seven gave solutions with our data. Nearly identical loss rates were obtained for /sup 155/Eu, /sup 239+240/Pu, and /sup 241/Am in the fast-exchanging compartments for all seven models. The uptake rates for these nuclides were also similar when uptake rates were normalized to local sediment concentrations. The fast-exchanging compartment probably corresponds to the mucilage surface layer of the coenocytic filaments. The identity of the slow-exchanging compartment is less certain but it may correspond to the skeletal surface.

  6. DOE Announces Webinars on Genetically Modified Algae, NREL’s Fuel Cell Contaminant Database, and More

    Broader source: Energy.gov [DOE]

    EERE offers webinars to the public on a range of subjects, from adopting the latest energy efficiency and renewable energy technologies to training for the clean energy workforce. View this week's webinars.

  7. Genomic and ecosystem evidence demonstrate the importance of selenium for the harmful alga, Aureococcus anophagefferens

    SciTech Connect (OSTI)

    Gobler, Christopher J; Lobanov, Alexei V; Tang, Ying-Zhong; Turanov, Anton A; Zhang, Yan; Doblin, Martina; Taylor, Gordon T; Sanudo-Wilhelmy, Sergio A; Grigoriev, Igor V; Gladyshev, Vadim N

    2012-10-19

    The trace element selenium (Se) is required for the biosynthesis of selenocysteine (Sec), the 21st amino acid in the genetic code, but its role in the ecology of harmful algal blooms (HABs) is unknown. Here, we examined the role of Se in the biology and ecology of the harmful pelagophyte, Aureococcus anophagefferens, through cell culture, genomic analyses, and ecosystem studies. This organism has the largest and the most diverse selenoproteome identified to date that consists of at least 59 selenoproteins, including known eukaryotic selenoproteins, selenoproteins previously only detected in bacteria, and novel selenoproteins. The A. anophagefferens selenoproteome was dominated by the thioredoxin fold proteins and oxidoreductase functions were assigned to the majority of detected selenoproteins. Insertion of Sec in these proteins was supported by a unique Sec insertion sequence. Se was required for the growth of A. anophagefferens as cultures grew maximally at nanomolar Se concentrations. In a coastal ecosystem, dissolved Se concentrations were elevated before and after A. anophagefferens blooms, but were reduced by >95percent during the peak of blooms to 0.05 nM. Consistent with this pattern, enrichment of seawater with selenite before and after a bloom did not affect the growth of A. anophagefferens, but enrichment during the peak of the bloom significantly increased population growth rates. These findings demonstrate that Se inventories, which can be anthropogenically enriched, can support proliferation of HABs, such as A. anophagefferens through its synthesis of a large arsenal of Se-dependent oxidoreductases that fine-tune cellular redox homeostasis.

  8. Microsoft Word - PhycalAlgaePilotProject_NEPAFinalEA_October2011.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Inert Gas Buffered Milling and Particle Size Separation of μm-Scale Superconducting Precursor Powders DOE SBIR Phase 2 Grant DE-FG02-05ER84344 Accelerator Technology Corp. 9701 Valley View Dr. College Station, TX77845 (979)255-5531 acctec@suddenlink.net Satya Seshadri, PI Final Report Executive summary The project developed an aerosol system for the met milling and particle size separation of the precursor powders used in fabrication of powder-in-tube superconductors. The work builds upon the

  9. BioenergizeME Virtual Science Fair: Is Algae the Next Big Thing

    Broader source: Energy.gov [DOE]

    This infographic was created by students from Daniel Boone Area High School in Birdsboro, PA, as part of the U.S. Department of Energy-BioenergizeME Virtual Science Fair.

  10. The Promise and Challenge of Algae as Renewable Sources of Biofuels

    Broader source: Energy.gov [DOE]

    This PDF focuses on the Biomass Program’s approach to algal biofuels research and development, and it includes presentations from four representatives of its recently funded consortia. This PDF also highlights from the National Algal Biofuels Technology Roadmap.

  11. 2016 Bioenergizeme Infographic Challenge: Algae Biofuels, Exploring the Energy of Tomorrow Today

    Office of Energy Efficiency and Renewable Energy (EERE)

    This infographic was created by students from Smithtown High School East in St. James, NY, as part of the U.S. Department of Energy-BioenergizeME Infographic Challenge. The BioenergizeME...

  12. Microsoft Word - PhycalAlgaePilotProject_NEPAFinalEA_October2011...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... quality and greenhouse gas, noise, geology and soils, ... of Hawaii Department of Land and Natural Resources DNA ... responsive to the requirements of the FOA. In ...

  13. Algae as a Feedstock for Transportation Fuels. The Future of Biofuels?

    SciTech Connect (OSTI)

    McGill, Ralph

    2008-05-15

    Events in world energy markets over the past several years have prompted many new technical developments as well as political support for alternative transportation fuels, especially those that are renewable. We have seen dramatic rises in the demand for and production of fuel ethanol from sugar cane and corn and biodiesel from vegetable oils. The quantities of these fuels being used continue to rise dramatically, and their use is helping to create a political climate for doing even more. But, the quantities are still far too small to stem the tide of rising crude prices worldwide. In fact, the use of some traditional crops (corn, sugar, soy, etc.) in making fuels instead of food is apparently beginning to impact the cost of food worldwide. Thus, there is considerable interest in developing alternative biofuel feedstocks for use in making fuels -- feedstocks that are not used in the food industries. Of course, we know that there is a lot of work in developing cellulosic-based ethanol that would be made from woody biomass. Process development is the critical path for this option, and the breakthrough in reducing the cost of the process has been elusive thus far. Making biodiesel from vegetable oils is a well-developed and inexpensive process, but to date there have been few reasonable alternatives for making biodiesel, although advanced processes such as gasification of biomass remain an option.

  14. "The Promise and Challenge of Algae as Renewable Sources of Biofuels...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This session also discussed highlights from the National Algal Biofuels Technology Roadmap... National Algal Biofuels Technology Roadmap 2015 Peer Review Presentations-Algal ...

  15. Development of Hydrothermal Liquefaction and Upgrading Technologies for Lipid-Extracted Algae Conversion to Liquid Fuels

    SciTech Connect (OSTI)

    Zhu, Yunhua; Albrecht, Karl O.; Elliott, Douglas C.; Hallen, Richard T.; Jones, Susanne B.

    2013-10-01

    Bench-scale tests were performed for lipid-extracted microalgae (LEA) conversion to liquid fuels via hydrotreating liquefaction (HTL) and upgrading processes. Process simulation and economic analysis for a large-scale LEA HTL and upgrading system were developed based on the best available test results. The system assumes an LEA feed rate of 608 dry metric ton/day and that the feedstock is converted to a crude HTL bio-oil and further upgraded via hydrotreating and hydrocracking to produce liquid hydrocarbon fuels, mainly alkanes. Performance and cost results demonstrate that HTL would be an effective option to convert LEA to liquid fuel. The liquid fuels annual yield was estimated to be 26.9 million gallon gasoline-equivalent and the overall energy efficiency at higher heating value basis was estimated to be 69.5%. The minimum fuel selling price (MFSP) was estimated to be $0.75/L with LEA feedstock price at $33.1 metric ton at dry basis and 10% internal rate of return. A sensitivity analysis indicated that the largest effects to production cost would come from the final products yields and the upgrading equipments cost. The impact of plant scale on MFSP was also investigated.

  16. Updated Cost Analysis of Photobiological Hydrogen Production from Chlamydomonas reinhardtii Green Algae: Milestone Completion Report

    Broader source: Energy.gov [DOE]

    This report updates the 1999 economic analysis of NREL’s photobiological hydrogen production from Chlamydomonas reinhardtii.

  17. Genomic Sequence of a Marine Blooming Alga | U.S. DOE Office of Science

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    vivo Low-Dose Low LET Ionizing Radiation Exposure: Pathways and Mechanisms Final Report, September 2013 (Technical Report) | SciTech Connect Genome Wide Evaluation of Normal Human Tissue in Response to Controlled, In vivo Low-Dose Low LET Ionizing Radiation Exposure: Pathways and Mechanisms Final Report, September 2013 Citation Details In-Document Search Title: Genome Wide Evaluation of Normal Human Tissue in Response to Controlled, In vivo Low-Dose Low LET Ionizing Radiation Exposure:

  18. Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Mansfeldt, Cresten B.; Richter, Lubna V.; Ahner, Beth A.; Cochlan, William P.; Richardson, Ruth E.; Chen, Shilin

    2016-02-03

    Here, marine chlorophytes of the genus Chlorella are unicellular algae capable of accumulating a high proportion of cellular lipids that can be used for biodiesel production. In this study, we examined the broad physiological capabilities of a subtropical strain (C596) of Chlorella sp. “SAG-211-18” including its heterotrophic growth and tolerance to low salt.We found that the alga replicates more slowly at diluted salt concentrations and can grow on a wide range of carbon substrates in the dark.We then sequenced the RNA of Chlorella strain C596 to elucidate key metabolic genes and investigate the transcriptomic response of the organism when transitioningmore » from a nutrient-replete to a nutrient-deficient condition when neutral lipids accumulate. Specific transcripts encoding for enzymes involved in both starch and lipid biosynthesis, among others, were up-regulated as the cultures transitioned into a lipid-accumulating state whereas photosynthesis-related genes were down-regulated. Transcripts encoding for two of the up-regulated enzymes—a galactoglycerolipid lipase and a diacylglyceride acyltransferase—were also monitored by reverse transcription quantitative polymerase chain reaction assays. The results of these assays confirmed the transcriptome-sequencing data. The present transcriptomic study will assist in the greater understanding, more effective application, and efficient design of Chlorella-based biofuel production systems.« less

  19. Advancing Commercialization of Algal Biofuels through Increased...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biomass Productivity and Technical Integration March 25, 2015 Algae Platform Review ... Algae Harvesting * Aft-H. Overall Integration and Scale-up * Aft-I. Algae Feedstock ...

  20. Categorical Exclusion Determinations: B5.23 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hawthorne Charging Station at Lady Bird Park Rest Area Award Number: DE-EE0006992 CX(s) ... Hawthorne Charging Station at Lady Bird Park Rest Area Award Number: DE-EE0006992 CX(s) ...

  1. FE Categorical Exclusions | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ENI USA Gas Marketing, LLC CX(s) Applied: B5.7 Date: 04142015 Location(s): Multiple ... ENI USA Gas Marketing, LLC CX(s) Applied: B5.7 Date: 04142015 Location(s): Multiple ...

  2. Categorical Exclusion Determinations: North Dakota | Department...

    Energy Savers [EERE]

    ... Carbon Dioxide Storage Efficiency in Deep Saline Formations CX(s) Applied: A9 Date: 0423... University of North Dakota- Novel Dry Cooling Technology for Power Plants CX(s) Applied: ...

  3. Categorical Exclusion Determinations: Naval Nuclear Propulsion...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Naval Reactors Facility (NRF) Storm Sewer West Main Replacement Project CX(s) Applied: ... Kesselring Site Gate 5 Project CX(s) Applied: B1.15 Date: 06052015 Location(s): None ...

  4. Categorical Exclusion Determinations: Indiana | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Plant Award Number: DE-EE0006244 CX(s) Applied: A9, B3.6 Bioenergy Technology Office ... Award Number: DE-EE0006210 CX(s) Applied: B5.1, B5.17 Weatherization & ...

  5. Categorical Exclusion Determinations: B1.23 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    E-Area Trailer and Handy House Demolition and Removal CX(s) Applied: B1.23 Date: 0520... Demolish and Dispose of Shed Located East of Test Reactor Area-607 CX(s) Applied: B1.23 ...

  6. Categorical Exclusion Determinations: Office of River Protection...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CHPRC Cleanup Actions CX(s) Applied: B6.1 Date: 41849 Location(s): Washington Offices(s): ... Waste Site Retrieval Pilot Project CX(s) Applied: B3.6 Date: 07092014 Location(s): ...

  7. Categorical Exclusion Determinations: Fossil Energy | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ConocoPhillips Alaska Natural Gas Corporation CX(s) Applied: B5.7 Date: 242016 ... Bear Head LNG Corporation and Bear Head LNG (USA) CX(s) Applied: B5.7 Date: 1282016 ...

  8. Categorical Exclusion Determinations: Advanced Technology Vehicles...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Reequipping and Engineering CX(s) Applied: B1.31, B5.1 Date: 09062011 ... Aptera All-Electric and Hybrid Electric Vehicles CX(s) Applied: B1.31, B5.1 Date: 0620...

  9. NREL Discovers Novel Protein Interaction in Green Algae that Suggests New Strategies to Improve Hydrogen Photoproduction (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2011-02-01

    A research team at the National Renewable Energy Laboratory (NREL) discovered a specific interaction between the protein ferredoxin - responsible for distributing reductants from photosynthesis to different metabolic pathways - and the HYDA2 hydrogenase, suggesting a role for HYDA2 in photohydrogen production.

  10. Cultivation of macroscopic marine algae and freshwater aquatic needs. Progress report, May 1, 1979-December 15, 1979

    SciTech Connect (OSTI)

    Ryther, J H

    1980-01-01

    Progress for the period May 1979 to December 1979 is reported in the following subject areas: (1) the ORCA clone of the red seaweed Gracilaria tikvakiae has now been grown continuously in tank culture for two years; (2) studies were continued on the culture of freshwater plants such as water hyacinth, pennywort, water lettuce, and duckweed; (3) the loss of water from evapotranspiration of freshwater plants was measured and compared with water loss from evaporation from open water; and (4) experiments were conducted to investigate the possibility of recycling the chemicals left in the solid and liquid residues following anaerobic digestion and methane production as a source of nutrients for new plant production. (ACR)

  11. Suppression of Tla1 gene expression for improved solar conversion efficiency and photosynthetic productivity in plants and algae

    DOE Patents [OSTI]

    Melis, Anastasios; Mitra, Mautusi

    2010-06-29

    The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA1 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in plants, e.g., green microalgae, under bright sunlight conditions.

  12. On the structural stability and catalytic properties of smectities pillared with RE-Al, Al-Ga polyoxications

    SciTech Connect (OSTI)

    Caballero, L.; Dominguez, J.M.; De los Santos, J.L.

    1995-12-01

    Pillaring of Smectite type clays offers the possibility to develop highly porous catalytic materials with surface acid properties, (1) their me in hydrotreating and FCC hydrocarbon processes has been extensively discussed (2) and still some research on the stabilization of the clays structure is being carried out. Therefore, the aim of the present work was to investigate thermal stability of Montmorillonite type clays, by means of pillaring with distinct polyoxications and by ion-exchanging and treating the clays under several conditions.

  13. Excellence in Bioenergy Innovation-A Presentation of 2015 R&D...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon rd100winnerswebinar20160121.pdf More Documents & Publications Whole Algae Hydrothermal Liquefaction Technology Pathway Whole Algae Hydrothermal Liquefaction ...

  14. CONTACT INFORMATION: Steve Karsjen Public Affairs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    particles. Thanks to these particles someday, you could be riding in a car powered by biodiesel made from algae. Why make fuel from algae? For starters, algae grow quickly, and...

  15. CX-008684: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Metaline Radio Station Upgrade Project CX(s) Applied: B1.19 Date: 07/11/2012 Location(s): Washington Offices(s): Bonneville Power Administration

  16. CX-100327 Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CX-100327 Categorical Exclusion Determination Proposed Rulemaking for Energy Conservation Standards for Commercial Water Heating Equipment RIN: 1904-AD34 CX(s)...

  17. Categorical Exclusion Determinations: B4.9 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    September 24, 2014 CX-012808: Categorical Exclusion Determination LURR 20140504 Ross Substation Comcast Fiber Installation CX(s) Applied: B4.9 Date: 41906 Location(s): ...

  18. CX-007382: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Compressed Natural Gas Manufacturing CX(s) Applied: B5.1 Date: 10/26/2011 Location(s): Wisconsin Offices(s): Golden Field Office

  19. CX-007650: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Control Room Consolidation CX(s) Applied: B2.2 Date: 12/29/2011 Location(s): South Carolina Offices(s): Savannah River Operations Office

  20. CX-011531: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Targhee Substation Land Acquisition CX(s) Applied: B1.24 Date: 11/05/2013 Location(s): Idaho Offices(s): Bonneville Power Administration

  1. CX-012790: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Haystack Butte Radio Site Land Acquisition CX(s) Applied: B1.24Date: 41939 Location(s): WashingtonOffices(s): Bonneville Power Administration

  2. CX-008250: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Geotechnical Core Drilling for USGS 138 CX(s) Applied: B3.1 Date: 04/18/2012 Location(s): Idaho Offices(s): Nuclear Energy

  3. CX-009850: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Pittsburgh Nanomaterials Preparation Lab CX(s) Applied: B3.6 Date: 01/29/2013 Location(s): Pennsylvania Offices(s): National Energy Technology Laboratory

  4. CX-007893: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    SunShot Massachusetts CX(s) Applied: A9, A11 Date: 02/10/2012 Location(s): Massachusetts Offices(s): Golden Field Office

  5. CX-012620: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Grout Preparation CX(s) Applied: B3.6Date: 41799 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  6. CX-010578: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Celilo Converter Station Upgrades CX(s) Applied: B4.11 Date: 07/25/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  7. CX-008547: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    St. Petersburg Solar Pilot Project CX(s) Applied: B5.16 Date: 05/31/2012 Location(s): Florida Offices(s): Golden Field Office

  8. CX-008884: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Rattlesnake Butte Property Funding CX(s) Applied: B1.25 Date: 08/13/2012 Location(s): Oregon Offices(s): Bonneville Power Administration

  9. CX-008307: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Deepwater Reverse-Circulation Primary Cementing CX(s) Applied: A9 Date: 04/25/2012 Location(s): Texas Offices(s): National Energy Technology Laboratory

  10. CX-010618: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Midwest Region Alternative Fuels Project CX(s) Applied: 0 Date: 07/19/2013 Location(s): Missouri Offices(s): National Energy Technology Laboratory

  11. CX-008799: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Jack Case Showers Projects CX(s) Applied: B1.3 Date: 06/04/2012 Location(s): Tennessee Offices(s): Y-12 Site Office

  12. CX-011177: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Hebo Substation Access Road Maintenance CX(s) Applied: B1.3 Date: 09/13/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  13. CX-011676: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Office Trailer Relocation CX(s) Applied: B1.22 Date: 12/09/2013 Location(s): South Carolina Offices(s): Savannah River Operations Office

  14. CX-008215: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Small Hydropower Research and Development Technology Project CX(s) Applied: A9 Date: 04/03/2012 Location(s): Colorado Offices(s): Golden Field Office

  15. CX-011194: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Particle Physics Division Outback Garage CX(s) Applied: B1.15 Date: 09/19/2013 Location(s): Illinois Offices(s): Fermi Site Office

  16. CX-012561: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Replace Department of Energy Office Trailers CX(s) Applied: B1.15Date: 41871 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  17. CX-011534: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Grays River Confluence Property Funding CX(s) Applied: B1.25 Date: 11/08/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  18. CX-011110: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Advancements in Algal Biomass Yield CX(s) Applied: A9 Date: 08/29/2013 Location(s): Hawaii Offices(s): Golden Field Office

  19. CX-010763: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CX-010763: Categorical Exclusion Determination Nevada Desert Research Institute- Photovoltaic Installation CX(s) Applied: B5.16 Date: 07172013 Location(s): Nevada Offices(s):...

  20. CX-009237: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    The Dow Chemical Company CX(s) Applied: B5.7 Date: 10/02/2012 Location(s): Texas Offices(s): Fossil Energy

  1. CX-013619: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Upper Lemhi Conservation Easement Acquisition CX(s) Applied: B1.25Date: 05/26/2015 Location(s): IdahoOffices(s): Bonneville Power Administration

  2. CX-008161: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Prosser Hatchery Backup Generator Replacement CX(s) Applied: B1.31 Date: 04/16/2012 Location(s): Washington Offices(s): Bonneville Power Administration

  3. CX-012463: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Reliable SOFC Systems CX(s) Applied: A9, B3.6Date: 41877 Location(s): ConnecticutOffices(s): National Energy Technology Laboratory

  4. CX-013624: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Ahtanum Creek Property Acquisition Funding CX(s) Applied: B1.25Date: 05/04/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  5. CX-012585: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Grit Blasting CX(s) Applied: B3.6Date: 41835 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  6. CX-013432: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Process Demonstration Unit Support Structure CX(s) Applied: B1.15Date: 02/04/2015 Location(s): IdahoOffices(s): Idaho Operations Office

  7. CX-008875: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Bingen Substation Sectionalizing Switches Disposition CX(s) Applied: B1.24 Date: 08/27/2012 Location(s): Washington Offices(s): Bonneville Power Administration

  8. CX-011401: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Minto Lodge Rehabilitation CX(s) Applied: B5.1 Date: 11/19/2013 Location(s): Alaska Offices(s): Golden Field Office

  9. CX-013450: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Ellensburg Guy Wire Installation CX(s) Applied: B4.9Date: 03/06/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  10. CX-013823: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Electric Vehicle Infrastructure (EVI) Laboratory Upgrades CX(s) Applied: B1.31Date: 06/18/2015 Location(s): IdahoOffices(s): Nuclear Energy

  11. CX-012789: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Building 440 CNM Clean Room Expansion CX(s) Applied: B3.15Date: 41906 Location(s): IllinoisOffices(s): Argonne Site Office

  12. CX-100400 Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Corrosion-resistant non-carbon electrocatalyst supports for PEFCs Award Number: DE- EE-0007272 CX(s) Applied: A9, B3.6

  13. CX-011822: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Light Willow Demonstration CX(s) Applied: B3.6 Date: 01/09/2014 Location(s): South Carolina Offices(s): Savannah River Operations Office

  14. Categorical Exclusion Determinations: Golden Field Office | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Categorical Exclusion Determination U.S. Department of Energy Collegiate Entrepreneurship Prize Award Number: TBD FOA CX(s) Applied: A9 Date: 12222014 Location(s): CO...

  15. CX-012656: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    North Bend Communication Site Engine Generator Replacement CX(s) Applied: B1.3Date: 41848 Location(s): WashingtonOffices(s): Bonneville Power Administration

  16. CX-006520: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CX-006520: Categorical Exclusion Determination High Energy Density Distributed Hydrostatic Direct Drive for Large Wind Turbine and Marine Hydro-Kinetic Device Applications CX(s) ...

  17. CX-003608: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CX-003608: Categorical Exclusion Determination Sustainable Algal Energy Production and Environmental Remediation CX(s) Applied: A9, B3.6 Date: 08252010 Location(s): Virginia ...

  18. CX-012278: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Building 36 Use Permit CX(s) Applied: A9 Date: 06/23/2014 Location(s): Oregon Offices(s): National Energy Technology Laboratory

  19. CX-012434: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Low Cost Titanium Casting Technology CX(s) Applied: B3.6Date: 41878 Location(s): OhioOffices(s): National Energy Technology Laboratory

  20. CX-006646: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Restoration South of 54-TPX-10CX(s) Applied: B6.1Date: 02/09/2010Location(s): Casper, WyomingOffice(s): RMOTC

  1. CX-010717: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Moodys Radio Tower Land Acquisition CX(s) Applied: B1.24 Date: 07/15/2013 Location(s): Oklahoma Offices(s): Southwestern Power Administration

  2. CX-010155: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Augspurger Radio Tower Replacement Project CX(s) Applied: B1.19 Date: 04/03/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  3. CX-008683: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Shaniko Radio Station Replacement Project CX(s) Applied: B1.19 Date: 07/11/2012 Location(s): Oregon Offices(s): Bonneville Power Administration

  4. CX-011190: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Alberton Communication Site Construction CX(s) Applied: B1.19 Date: 08/26/2013 Location(s): Montana Offices(s): Bonneville Power Administration

  5. CX-011237: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Lightspeed Networks Inc. Fiber Installation CX(s) Applied: B4.9 Date: 10/24/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  6. CX-012803: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Sacajawea Substation Expansion and Upgrade CX(s) Applied: B4.6Date: 41912 Location(s): WashingtonOffices(s): Bonneville Power Administration

  7. CX-012002: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Raver-Covington Conductor Replacement CX(s) Applied: B1.3 Date: 04/24/2014 Location(s): Washington Offices(s): Bonneville Power Administration

  8. CX-009423: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Relay and Switchboard Panel Replacements CX(s) Applied: B4.6 Date: 10/29/2012 Location(s): Arkansas Offices(s): Southwestern Power Administration

  9. CX-011214: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Sensitive Instrument Facility CX(s) Applied: B3.6 Date: 07/10/2013 Location(s): Iowa Offices(s): Ames Site Office

  10. CX-009398: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Domestic Source Recovery CX(s) Applied: B2.6 Date: 11/01/2012 Location(s): New Mexico Offices(s): Los Alamos Site Office

  11. CX-007587: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Gas Chromatography CX(s) Applied: B3.6 Date: 12/29/2011 Location(s): Oregon Offices(s): National Energy Technology Laboratory

  12. CX-010124: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chromatography / Mass Spectrometry CX(s) Applied: B3.6 Date: 03/20/2013 Location(s): South Carolina Offices(s): Savannah River Operations Office

  13. CX-007399: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Offshore Wind Removing Market Barriers CX(s) Applied: A9, A11 Date: 12/20/2011 Location(s): Massachusetts Offices(s): Golden Field Office

  14. Categorical Exclusion Determinations: A12 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    September 29, 2011 CX-006906: Categorical Exclusion Determination Electricity Delivery and Energy Reliability Assistance to State Regulatory Utility Commissioners CX(s) Applied: ...

  15. CX-010195: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Polymer Synthesis Lab - Modification CX(s) Applied: B3.6 Date: 04/15/2013 Location(s): Pennsylvania Offices(s): National Energy Technology Laboratory

  16. CX-012437: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    High Energy Density Lithium Battery CX(s) Applied: B3.6Date: 41878 Location(s): New YorkOffices(s): National Energy Technology Laboratory

  17. CX-012028: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    High Temperature Laboratory CX(s) Applied: B3.6 Date: 04/21/2014 Location(s): Oregon Offices(s): National Energy Technology Laboratory

  18. CX-013534: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    ENI USA Gas Marketing, LLC CX(s) Applied: B5.7Date: 04/14/2015 Location(s): Multiple LocationsOffices(s): Fossil Energy

  19. CX-009797: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Eni USA Gas Marketing, LLC CX(s) Applied: B5.7 Date: 02/06/2013 Location(s): Louisiana Offices(s): Fossil Energy

  20. CX-001627: Categorical Exclusion Determination | Department of...

    Office of Environmental Management (EM)

    CX-001627: Categorical Exclusion Determination Test Reactor Cask Implementation CX(s) ... This proposed action is a process and facility modification. The Advanced Test Reactor ...

  1. CX-010338: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Eugene Substation Fiber Interconnection CX(s) Applied: B4.7 Date: 05/21/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  2. CX-010435: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    De Moss Substation Expansion CX(s) Applied: B4.6 Date: 06/03/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  3. CX-008146: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Advanced Formation Evaluator Tools (Haliburton) CX(s) Applied: B3.7 Date: 09/11/2011 Location(s): Wyoming Offices(s): RMOTC

  4. Categorical Exclusion Determinations: Idaho | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... June 18, 2015 CX-013839: Categorical Exclusion Determination Materials and Fuels Complex (MFC)-752 Analytical Laboratory Casting Laboratory Glovebox Heat Detection CX(s) Applied: ...

  5. CX-006681: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    New Drilling Location in Section 29CX(s) Applied: B3.1Date: 12/23/2009Location(s): Casper, WyomingOffice(s): RMOTC

  6. CX-009418: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Electron Beam Melting CX(s) Applied: None applied. Date: 10/30/2012 Location(s): Missouri Offices(s): Kansas City Site Office

  7. CX-013721: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Solar Vacuum Furnace CX(s) Applied: B3.6Date: 04/28/2015 Location(s): OregonOffices(s): National Energy Technology Laboratory

  8. CX-012188: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Emerald Ash Borer Control CX(s) Applied: B1.3 Date: 05/06/2014 Location(s): Illinois Offices(s): Argonne Site Office

  9. CX-011065: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Midwest Region Alternative Fuels Project CX(s) Applied: A1 Date: 08/29/2013 Location(s): Kansas Offices(s): National Energy Technology Laboratory

  10. CX-011788: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    I-75 Green Corridor Project CX(s) Applied: A1 Date: 02/10/2014 Location(s): Tennessee Offices(s): National Energy Technology Laboratory

  11. CX-007497: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Clean Energy Coalition - Michigan Green Fleets CX(s) Applied: A1 Date: 12/06/2011 Location(s): Michigan Offices(s): National Energy Technology Laboratory

  12. CX-010938: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Midwest Region Alternative Fuels Project CX(s) Applied: A1 Date: 09/17/2013 Location(s): Kansas, Kansas Offices(s): National Energy Technology Laboratory

  13. CX-011271: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Idaho Petroleum Reduction Leadership Project CX(s) Applied: A1 Date: 09/30/2013 Location(s): Idaho Offices(s): National Energy Technology Laboratory

  14. CX-008588: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    St. Petersburg Solar Pilot Project CX(s) Applied: B5.1 Date: 07/19/2012 Location(s): Florida Offices(s): Golden Field Office

  15. CX-011634: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Closure Turf Installation CX(s) Applied: B6.1 Date: 08/27/2013 Location(s): Texas Offices(s): Pantex Site Office

  16. CX-008803: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Milling Machine Replacement Projects CX(s) Applied: B1.31 Date: 05/14/2012 Location(s): Tennessee Offices(s): Y-12 Site Office

  17. CX-012725: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Materials and Fuel Complex (MFC)-782 Fire Sprinkler Installation CX(s) Applied: B2.2Date: 41829 Location(s): IdahoOffices(s): Nuclear Energy

  18. CX-010756: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Solar Utility Network Deployment Acceleration CX(s) Applied: A9, A11 Date: 08/15/2013 Location(s): Virginia Offices(s): Golden Field Office

  19. CX-011102: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    American Solar Transformation Initiative CX(s) Applied: A11 Date: 08/09/2013 Location(s): California Offices(s): Golden Field Office

  20. CX-012531: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Distributed Wireless Antenna Sensors for Boiler Condition CX(s) Applied: B3.6Date: 41836 Location(s): CaliforniaOffices(s): National Energy Technology Laboratory

  1. CX-012539: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Distributed Wireless Antenna Sensors for Boiler Condition CX(s) Applied: B3.6Date: 41836 Location(s): TexasOffices(s): National Energy Technology Laboratory

  2. CX-008984: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    State Energy Program CX(s) Applied: A9, A11 Date: 08/29/2012 Location(s): Florida Offices(s): Golden Field Office

  3. CX-012798: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Davis Creek Tap Wood Pole Replacements CX(s) Applied: B1.3Date: 41915 Location(s): CaliforniaOffices(s): Bonneville Power Administration

  4. CX-007550: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Kearney - Waste Water Treatment Plant CX(s) Applied: B5.1 Date: 01/10/2012 Location(s): Missouri Offices(s): Golden Field Office

  5. CX-007549: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Harrisonville - Waste Water Treatment Plant CX(s) Applied: B5.1 Date: 01/10/2012 Location(s): Missouri Offices(s): Golden Field Office

  6. CX-009420: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Additive Manufacturing Using EOSINT M280 CX(s) Applied: None applied. Date: 10/30/2012 Location(s): Missouri Offices(s): Kansas City Site Office

  7. CX-008700: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Natapoc Property Funding CX(s) Applied: B1.25 Date: 06/12/2012 Location(s): Washington Offices(s): Bonneville Power Administration

  8. CX-009028: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Wisconsin Biodiesel Blending Program CX(s) Applied: B5.22 Date: 08/22/2012 Location(s): Wisconsin Offices(s): Golden Field Office

  9. CX-007856: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Sacramento Regional Energy Alliance CX(s) Applied: B5.23 Date: 01/27/2012 Location(s): California Offices(s): Golden Field Office

  10. CX-011625: Categorical Exclusion Determinationc

    Broader source: Energy.gov [DOE]

    9103 Second Floor Refurbishment CX(s) Applied: B1.3 Date: 06/05/2013 Location(s): Tennessee Offices(s): Y-12 Site Office

  11. CX-012495: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Building 6 Stack Replacement CX(s) Applied: B1.3Date: 41855 Location(s): West VirginiaOffices(s): National Energy Technology Laboratory

  12. CX-013324: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Wear Laboratory CX(s) Applied: A9, B3.6Date: 01/16/2015 Location(s): OregonOffices(s): National Energy Technology Laboratory

  13. CX-007423: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Highland Independent School District CX(s) Applied: B5.18 Date: 12/13/2011 Location(s): Texas Offices(s): Golden Field Office

  14. CX-007428: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Ralls Independent School District CX(s) Applied: B5.18 Date: 12/20/2011 Location(s): Texas Offices(s): Golden Field Office

  15. CX-004649: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Painesville Municipal Power Vanadium Redox Battery Demonstration Program CX(s) ... CX-006145: Categorical Exclusion Determination Fact Sheet: Vanadium Redox Battery ...

  16. CX-010734: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Covington District Culvert Replacements CX(s) Applied: B1.3 Date: 07/22/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  17. CX-012641: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Mossy Rock-Chehalis #1 Access Road Maintenance CX(s) Applied: B1.3Date: 41865 Location(s): WashingtonOffices(s): Bonneville Power Administration

  18. CX-012799: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Malin-Hilltop Wood Pole Replacements CX(s) Applied: B1.3Date: 41915 Location(s): CaliforniaOffices(s): Bonneville Power Administration

  19. CX-012805: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Brasada-Harney #1 Wood Pole Replacements CX(s) Applied: B1.3Date: 41908 Location(s): OregonOffices(s): Bonneville Power Administration

  20. CX-008989: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    State Energy Program CX(s) Applied: A9, A11 Date: 08/27/2012 Location(s): Kansas Offices(s): Golden Field Office

  1. CX-008144: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Planned Repair of Flow Lines CX(s) Applied: B5.4 Date: 08/09/2011 Location(s): Wyoming Offices(s): RMOTC

  2. CX-011564: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Excess Facilities Deactivation and Demolition CX(s) Applied: B1.23 Date: 11/05/2013 Location(s): Idaho Offices(s): Idaho Operations Office

  3. CX-012816: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Rogue-Gold Beach Access Road Improvement CX(s) Applied: B1.3Date: 41890 Location(s): OregonOffices(s): Bonneville Power Administration

  4. CX-008724: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Rexburg Bus Lot Lease Termination CX(s) Applied: B1.24 Date: 07/05/2012 Location(s): Idaho Offices(s): Idaho Operations Office

  5. CX-009513: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Aquatic Invasive Mussels Monitoring CX(s) Applied: B3.1 Date: 10/15/2012 Location(s): CX: none Offices(s): Bonneville Power Administration

  6. CX-013632: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Alcoa Power Sales Contract Amendment CX(s) Applied: B4.11Date: 04/23/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  7. CX-012110: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Cowlitz Falls Fish Facility Access Agreement Extension CX(s) Applied: A2 Date: 04/02/2014 Location(s): Washington Offices(s): Bonneville Power Administration

  8. CX-009707: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Alcoa Power Sales Contract CX(s) Applied: B4.1 Date: 12/04/2012 Location(s): Oregon, Washington Offices(s): Bonneville Power Administration

  9. CX-012283: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    USC Autoclave CX(s) Applied: B3.6 Date: 06/14/2014 Location(s): Oregon Offices(s): National Energy Technology Laboratory

  10. CX-009312: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Pecan Street Smart Grid Extension Service CX(s) Applied: A9 Date: 08/30/2012 Location(s): Texas Offices(s): National Energy Technology Laboratory

  11. CX-007880: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Evergreen State Solar Partnership CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): Washington Offices(s): Golden Field Office

  12. CX-007859: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Developing Solar Friendly Communities CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): Colorado Offices(s): Golden Field Office

  13. CX-007869: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Solar Ready KC CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): Missouri Offices(s): Golden Field Office

  14. CX-007853: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Arizona Rooftop Challenge (ARC) CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): Arizona Offices(s): Golden Field Office

  15. CX-007882: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Wisconsin Solar Market Transformation CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): Wisconsin Offices(s): Golden Field Office

  16. CX-007858: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Southwest Solar Transformation Initiative CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): California Offices(s): Golden Field Office

  17. CX-007864: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Broward County SOLAR Project CX(s) Applied: A9, A11 Date: 01/27/2012 Location(s): Florida Offices(s): Golden Field Office

  18. CX-010426: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Vista View Fields Land Acquisition CX(s) Applied: B1.25 Date: 06/19/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  19. CX-010433: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Memaloose Meadows Land Acquisition CX(s) Applied: B1.25 Date: 06/04/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  20. CX-007833: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Energy Retrofits CX(s) Applied: B5.1 Date: 11/29/2011 Location(s): Colorado Offices(s): Energy Efficiency and Renewable Energy

  1. CX-009704: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Pasco Land Acquisition CX(s) Applied: B1.24 Date: 12/17/2012 Location(s): Washington Offices(s): Bonneville Power Administration

  2. CX-010148: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Merritt Radio Station Upgrade CX(s) Applied: B1.19 Date: 04/18/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  3. CX-010532: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Various Demolition Jobs CX(s) Applied: B1.23 Date: 06/07/2013 Location(s): Illinois Offices(s): Fermi Site Office

  4. CX-011215: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Nepese Marsh Upgrades CX(s) Applied: B2.5 Date: 10/17/2013 Location(s): Illinois Offices(s): Fermi Site Office

  5. CX-011642: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Pantex Lake Land Utilization CX(s) Applied: B1.11 Date: 11/05/2013 Location(s): Texas Offices(s): Pantex Site Office

  6. CX-011069: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Induction Furnace Melting CX(s) Applied: B3.6 Date: 08/29/2013 Location(s): Oregon Offices(s): National Energy Technology Laboratory

  7. CX-006211: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Exclusion Determination Missouri Independent Energy Efficiency Program: Henniges Automotive - Process Air Compressor Upgrades CX(s) Applied: B5.1 Date: 07182011 Location(s):...

  8. CX-013404: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CX-013404: Categorical Exclusion Determination NARUCDOE Natural Gas Infrastructure and Liquefied Natural Gas Partnership CX(s) Applied: A9, A11 Date: 02092015 Location(s): ...

  9. CX-012313: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chicago Office Technical Support Services Contract CX(s) Applied: A8 Date: 06/13/2014 Location(s): CX: none Offices(s): Chicago Office

  10. CX-009419: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Magnetic Pulser CX(s) Applied: None applied. Date: 10/30/2012 Location(s): Missouri Offices(s): Kansas City Site Office

  11. CX-010797: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Serration Behavior of High Entropy Alloys CX(s) Applied: A9 Date: 08/14/2013 Location(s): Illinois Offices(s): National Energy Technology Laboratory

  12. CX-012658: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chief Joseph and Custer Substations Security Fence Replacement CX(s) Applied: B1.11Date: 41843 Location(s): WashingtonOffices(s): Bonneville Power Administration

  13. CX-012776: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Catalyst Processing, KCP14-05 CX(s) Applied: NOT NOTEDDate: 41857 Location(s): MissouriOffices(s): Kansas City Site Office

  14. CX-013464: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Bond Strength Laser Shock Testing CX(s) Applied: B1.31Date: 02/18/2015 Location(s): IdahoOffices(s): Nuclear Energy

  15. CX-012728: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    BHP-3 Offsite Bump Repair CX(s) Applied: B1.3Date: 41885 Location(s): TexasOffices(s): Strategic Petroleum Reserve Field Office

  16. CX-001856: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Exclusion Determination Rural Cooperative Geothermal Development Electric and Agriculture CX(s) Applied: B3.1 Date: 04282010 Location(s): Paisley, Oregon Office(s): Energy...

  17. CX-012498: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Advanced Light Extraction Structure for OLED Lighting CX(s) Applied: B3.6Date: 41852 Location(s): MarylandOffices(s): National Energy Technology Laboratory

  18. CX-012512: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Amber Kinetics Flywheel Energy Storage Demonstration CX(s) Applied: B3.6Date: 41848 Location(s): CaliforniaOffices(s): National Energy Technology Laboratory

  19. CX-012333: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Support Buildings CX(s) Applied: B1.15 Date: 06/03/2014 Location(s): Washington Offices(s): River Protection-Richland Operations Office

  20. CX-008341: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    A-6 Office Building CX(s) Applied: B1.15 Date: 04/19/2012 Location(s): Pennsylvania Offices(s): Naval Nuclear Propulsion Program

  1. CX-012653: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Holcomb-Naselle #1 Access Road Improvements CX(s) Applied: B1.3Date: 41855 Location(s): WashingtonOffices(s): Bonneville Power Administration

  2. CX-012643: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chehalis-Covington #1 Access Roads CX(s) Applied: B1.13Date: 41865 Location(s): WashingtonOffices(s): Bonneville Power Administration

  3. Categorical Exclusion Determinations: B1.24 | Department of Energy

    Office of Environmental Management (EM)

    Offices(s): Bonneville Power Administration October 23, ... Grizzly Captain Jack Transmission Line Access Road ... Acquisition of Access Road Easements CX(s) Applied: B1.24 ...

  4. CX-012791: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Grizzly Captain Jack Transmission Line Access Road Acquisition CX(s) Applied: B1.24Date: 41935 Location(s): OregonOffices(s): Bonneville Power Administration

  5. CX-100159 Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Proposed Rulemaking for Energy Conservation Standards for Commercial and Industrial Pumps RIN: 1904-AC54 CX(s) Applied: B5.1

  6. CX-010019: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Iodine Speciation CX(s) Applied: B3.6 Date: 01/28/2013 Location(s): South Carolina Offices(s): Savannah River Operations Office

  7. CX-012054: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Catalyst Synthesis CX(s) Applied: B3.6 Date: 03/18/2014 Location(s): South Carolina Offices(s): Savannah River Operations Office

  8. CX-013604: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Corrosion Testing CX(s) Applied: B3.6Date: 03/05/2015 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  9. CX-013389: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Plutonium Characteristics CX(s) Applied: B3.6Date: 01/07/2015 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  10. CX-013332: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Magnesium Chloride Testing CX(s) Applied: B3.6Date: 12/15/2014 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  11. CX-013513: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Heavy Mineral Separation CX(s) Applied: B3.6Date: 02/05/2015 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  12. CX-013521: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chemical Oxygen Demand CX(s) Applied: B3.6Date: 02/03/2015 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  13. CX-007417: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Shift CX(s) Applied: B5.1 Date: 12/21/2011 Location(s): Pennsylvania Offices(s): Golden Field Office

  14. CX-012793: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    15-Minute Transmission Scheduling CX(s) Applied: B4.4, B4.5Date: 41933 Location(s): WashingtonOffices(s): Bonneville Power Administration

  15. CX-003703: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Determination Florida Hydrogen Initiative - Florida Institute of Technology (Interdisciplinary Hydrogen and Fuel Cell Technology Academic Program) CX(s) Applied: A9 Date: 09...

  16. CX-010770: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Wildland Fire Chainsaw Training CX(s) Applied: B1.2 Date: 08/01/2013 Location(s): Idaho Offices(s): Nuclear Energy

  17. CX-010091: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Power Line Configuration 2013-1 CX(s) Applied: B4.13 Date: 04/15/2012 Location(s): Idaho Offices(s): Nuclear Energy

  18. CX-010398: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Power Line Configuration CX(s) Applied: B4.13 Date: 04/25/2013 Location(s): Idaho Offices(s): Idaho Operations Office

  19. CX-010241: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Hydrogen Pathway Analyses CX(s) Applied: A9 Date: 02/28/2013 Location(s): Virginia Offices(s): Golden Field Office

  20. CX-010772: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Water Security Test Bed (WSTB) CX(s) Applied: B3.6 Date: 07/17/2013 Location(s): Idaho Offices(s): Nuclear Energy

  1. CX-012647: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Kalispell District 2014 Transmission Line Maintenance - Multiple Lines CX(s) Applied: B1.3Date: 41862 Location(s): MontanaOffices(s): Bonneville Power Administration

  2. CX-010727: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Dayton Tap Line Retirement CX(s) Applied: B4.10 Date: 08/13/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  3. CX-007522: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Crane Removal Project CX(s) Applied: B1.23 Date: 12/15/2011 Location(s): Tennessee Offices(s): Y-12 Site Office

  4. CX-013780: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Troy Substation Tap Yard Expansion CX(s) Applied: B4.11Date: 07/14/2015 Location(s): MontanaOffices(s): Bonneville Power Administration

  5. CX-013629: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Oregon City Station Service Replacement CX(s) Applied: B1.3Date: 04/27/2015 Location(s): OregonOffices(s): Bonneville Power Administration

  6. CX-013651: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Sappho Substation Expansion CX(s) Applied: B4.6Date: 04/07/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  7. CX-013310: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chemawa Substation Equipment Acquisition CX(s) Applied: B1.7Date: 01/06/2015 Location(s): OregonOffices(s): Bonneville Power Administration

  8. CX-013625: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Southern Cross Fee Title Funding CX(s) Applied: B1.25Date: 04/30/2015 Location(s): OregonOffices(s): Bonneville Power Administration

  9. CX-013796: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chehalis District Access Road Maintenance CX(s) Applied: B4.6Date: 06/15/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  10. CX-013794: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Horn Rapids Tap Disconnect Additions CX(s) Applied: B4.6Date: 06/17/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  11. CX-009587: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    City of Houston, Texas CX(s) Applied: B5.1 Date: 12/12/2012 Location(s): Texas Offices(s): Golden Field Office

  12. CX-010150: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Celilo Fiber System CX(s) Applied: B4.7 Date: 04/15/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  13. CX-013353: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Motor Silencer Installation CX(s) Applied: B1.21Date: 12/02/2014 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  14. CX-009630: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    ICP Routine Maintenance CX(s) Applied: B1.3 Date: 11/06/2012 Location(s): Idaho Offices(s): Idaho Operations Office

  15. CX-010768: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    ZIRCEX Nuclear Fuel Dissolution Testing CX(s) Applied: B3.6 Date: 08/12/2013 Location(s): Idaho Offices(s): Nuclear Energy

  16. CX-011250: Categorical Exclusion Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Determination Transforming Photovoltaic Installations Toward Dispatchable, Schedulable Energy Solutions CX(s) Applied: B3.6, B5.15 Date: 10172013 Location(s): Oregon...

  17. CX-012655: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Acquisition of Access Road Easements CX(s) Applied: B1.24Date: 41849 Location(s): WashingtonOffices(s): Bonneville Power Administration

  18. CX-009210: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Silver Butte Fiber Burial Project CX(s) Applied: B.47 Date: 08/28/2012 Location(s): Montana, Montana Offices(s): Bonneville Power Administration

  19. CX-012122: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    OCGen Module Mooring Project CX(s) Applied: B5.25 Date: 04/29/2014 Location(s): Maine Offices(s): Golden Field Office

  20. CX-010869: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Nauticas Research Program CX(s) Applied: B3.6 Date: 08/07/2013 Location(s): Illinois Offices(s): Argonne Site Office

  1. CX-007957: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Geothermal Incentive Program CX(s) Applied: B5.1 Date: 01/30/2012 Location(s): Connecticut Offices(s): National Energy Technology Laboratory

  2. CX-010341: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chandler Tap Line Reconductoring CX(s) Applied: B4.6 Date: 05/17/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  3. CX-010343: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Bald Hill Farms Property Funding CX(s) Applied: B1.25 Date: 05/10/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  4. CX-007779: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Routine Maintenance CX(s) Applied: B1.3 Date: 01/13/2012 Location(s): Washington Offices(s): River Protection-Richland Operations Office

  5. CX-007095: Categorical Exclusion Determination | Department of...

    Office of Environmental Management (EM)

    Determination CX-007095: Categorical Exclusion Determination Paving the Way with Propane: The AutoGas Corridor Development Program CX(s) Applied: B5.1 Date: 10132011...

  6. CX-007096: Categorical Exclusion Determination | Department of...

    Office of Environmental Management (EM)

    Determination CX-007096: Categorical Exclusion Determination Paving the Way with Propane: The AutoGas Corridor Development Program CX(s) Applied: B5.1 Date: 10132011...

  7. CX-012640: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Lexington-Longview #1 Access Road Maintenance CX(s) Applied: B1.3Date: 41865 Location(s): WashingtonOffices(s): Bonneville Power Administration

  8. CX-009005: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Henderson Solar Energy Project CX(s) Applied: B5.16 Date: 08/22/2012 Location(s): Nevada Offices(s): Golden Field Office

  9. CX-013423: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Kalispell Maintenance Headquarters Upgrades CX(s) Applied: B1.3Date: 01/28/2015 Location(s): MontanaOffices(s): Bonneville Power Administration

  10. CX-007939: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Midwest Region Alternative Fuels Project CX(s) Applied: A1 Date: 02/16/2012 Location(s): Missouri Offices(s): National Energy Technology Laboratory

  11. CX-013319: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Advanced Test Reactor (ATR) Complex Landscaping CX(s) Applied: B5.1Date: 12/16/2014 Location(s): IdahoOffices(s): Nuclear Energy

  12. CX-013463: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Advanced Test Reactor Air Conditioning Upgrades CX(s) Applied: B1.4Date: 02/19/2015 Location(s): IdahoOffices(s): Nuclear Energy

  13. CX-012724: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Advanced Test Reactor (ATR) Electronic Message Board Installation CX(s) Applied: B1.7Date: 41830 Location(s): IdahoOffices(s): Nuclear Energy

  14. CX-011369: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Relief Valve Test Stand Relocation CX(s) Applied: B1.31 Date: 10/24/2013 Location(s): Idaho Offices(s): Idaho Operations Office

  15. CX-012722: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Test Reactor Area (TRA)-653 Conference Room Modifications CX(s) Applied: B1.15Date: 41829 Location(s): IdahoOffices(s): Nuclear Energy

  16. Categorical Exclusion Determinations: A9 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    publication and distribution, and classroom training and informational programs), ... CX(s) Applied: A9 Federal Energy Management Program Date: 04132016 Location(s): ...

  17. Categorical Exclusion Determinations: Nuclear Energy | Department...

    Energy Savers [EERE]

    ... June 17, 2015 CX-013826: Categorical Exclusion Determination Assessment of Aging Degradation Mechanisms of Alloy 709 for Sodium Fast Reactors - Colorado School of Mines CX(s) ...

  18. CX-012311: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Accelerator Test Facility II CX(s) Applied: B3.10 Date: 05/28/2014 Location(s): New York Offices(s): Brookhaven Site Office

  19. CX-012200: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Determination of Excess Real Property CX(s) Applied: B1.36 Date: 05/01/2014 Location(s): Colorado Offices(s): Legacy Management

  20. CX-013605: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Electrochemistry Corrosion Testing CX(s) Applied: B3.6Date: 03/05/2015Location(s): South CarolinaOffices(s): Savannah River Operations Office

  1. CX-010733: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Port of Morrow Land Acquisition CX(s) Applied: B1.24 Date: 07/31/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  2. CX-008168: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Snohomish High School Field Improvements CX(s) Applied: B4.9 Date: 03/20/2012 Location(s): Washington Offices(s): Bonneville Power Administration

  3. CX-010586: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Umatilla Electric Cooperative CX(s) Applied: B4.9 Date: 07/03/2013 Location(s): Oregon Offices(s): Bonneville Power Administration

  4. CX-012648: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Bonneville Power House-Alcoa #2 Tower Relocation CX(s) Applied: B1.3Date: 41857 Location(s): WashingtonOffices(s): Bonneville Power Administration

  5. CX-012818: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    La Pine-Chiloquin Wood Pole Replacements CX(s) Applied: B1.3Date: 41887 Location(s): OregonOffices(s): Bonneville Power Administration

  6. CX-010588: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Chehalis Substation Tree Clearing CX(s) Applied: B1.3 Date: 07/02/2013 Location(s): Washington Offices(s): Bonneville Power Administration

  7. CX-009796: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Sempra Liquid Natural Gas Marketing, LLC CX(s) Applied: B5.7 Date: 01/15/2013 Location(s): Louisiana Offices(s): Fossil Energy

  8. CX-009533: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Sempra LNG Marketing, Inc. CX(s) Applied: B5.7 Date: 01/15/2013 Location(s): California, Louisiana Offices(s): Fossil Energy

  9. CX-009027: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Town of Franklin Weatherization Retrofits CX(s) Applied: B5.1 Date: 08/20/2012 Location(s): Vermont Offices(s): Golden Field Office

  10. CX-012807: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Action Substation Equipment Sale CX(s) Applied: B1.24Date: 41906 Location(s): OregonOffices(s): Bonneville Power Administration

  11. CX-013446: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Longview Substation Sanitary Sewer Upgrade CX(s) Applied: B4.9Date: 03/25/2015 Location(s): WashingtonOffices(s): Bonneville Power Administration

  12. CX-012809: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    LURR 20140313 City of Vancouver Sewer Lateral CX(s) Applied: B4.9Date: 41906 Location(s): WashingtonOffices(s): Bonneville Power Administration

  13. CX-013655: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Dark Canyon Conservation Easement Acquisition CX(s) Applied: B1.25Date: 03/27/2015 Location(s): OregonOffices(s): Bonneville Power Administration

  14. CX-008556: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Haiti Renewable Resource Study CX(s) Applied: A9, A11 Date: 07/23/2012 Location(s): Haiti Offices(s): Golden Field Office

  15. CX-008926: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Texas Alternative Fuel Vehicle Pilot Program CX(s) Applied: A1 Date: 08/24/2012 Location(s): Texas Offices(s): National Energy Technology Laboratory

  16. CX-011127: Categorical Exclusion Determination | Department of...

    Energy Savers [EERE]

    Natural Refrigerant High Performance Heat Pump for Commercial Applications CX(s) Applied: ... an air source heat pump targeted for heating, ventilation, and air conditioning purposes. ...

  17. CX-010234: Categorical Exclusion Determination | Department of...

    Energy Savers [EERE]

    Alternate Spacing Heating Systems Residential Cold Climate Heat Pump CX(s) Applied: A9, ... C Climate Heat Pump (CCHP) using boosted compression technology and variable speed motors. ...

  18. CX-008179: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Building 09-056 Demolition CX(s) Applied: B1.23 Date: 04/24/2012 Location(s): Texas Offices(s): Pantex Site Office

  19. CX-012563: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Roof repairs at 735-A CX(s) Applied: B1.3Date: 41870 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  20. CX-011679: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Antifoam Degradation Testing CX(s) Applied: B3.6 Date: 12/05/2013 Location(s): South Carolina Offices(s): Savannah River Operations Office