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Sample records for haven wild hare

  1. hare-98.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Diurnal Variability of Turbulent Fluxes in the Equatorial Pacific J. E. Hare Cooperative Institute for Research in Environmental Sciences University of Colorado Boulder, Colorado C. W. Fairall NOAA-Environmental Technology Laboratory Boulder, Colorado Introduction During March and April of 1996, scientists from 13 national research institutions participated in the Combined Sensor Program (CSP) experiment. This program is a collaborative effort of the U.S. Department of Energy (DOE) and the

  2. HARE: Final Report

    SciTech Connect (OSTI)

    Mckie, Jim

    2012-01-09

    This report documents the results of work done over a 6 year period under the FAST-OS programs. The first effort was called Right-Weight Kernels, (RWK) and was concerned with improving measurements of OS noise so it could be treated quantitatively; and evaluating the use of two operating systems, Linux and Plan 9, on HPC systems and determining how these operating systems needed to be extended or changed for HPC, while still retaining their general-purpose nature. The second program, HARE, explored the creation of alternative runtime models, building on RWK. All of the HARE work was done on Plan 9. The HARE researchers were mindful of the very good Linux and LWK work being done at other labs and saw no need to recreate it. Even given this limited funding, the two efforts had outsized impact: _ Helped Cray decide to use Linux, instead of a custom kernel, and provided the tools needed to make Linux perform well _ Created a successor operating system to Plan 9, NIX, which has been taken in by Bell Labs for further development _ Created a standard system measurement tool, Fixed Time Quantum or FTQ, which is widely used for measuring operating systems impact on applications _ Spurred the use of the 9p protocol in several organizations, including IBM _ Built software in use at many companies, including IBM, Cray, and Google _ Spurred the creation of alternative runtimes for use on HPC systems _ Demonstrated that, with proper modifications, a general purpose operating systems can provide communications up to 3 times as effective as user-level libraries Open source was a key part of this work. The code developed for this project is in wide use and available at many places. The core Blue Gene code is available at https://bitbucket.org/ericvh/hare. We describe details of these impacts in the following sections. The rest of this report is organized as follows: First, we describe commercial impact; next, we describe the FTQ benchmark and its impact in more detail; operating systems and runtime research follows; we discuss infrastructure software; and close with a description of the new NIX operating system, future work, and conclusions.

  3. Veteran's Affairs Health Care System, West Haven, Connecticut...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Veteran's Affairs Health Care System, West Haven, Connecticut Veteran's Affairs Health Care System, West Haven, Connecticut Overview The West Haven (Connecticut) Campus of the...

  4. Safe-haven locking device

    DOE Patents [OSTI]

    Williams, J.V.

    1984-04-26

    Disclosed is a locking device for eliminating external control of a secured space formed by fixed and movable barriers. The locking device uses externally and internally controlled locksets and a movable strike, operable from the secured side of the movable barrier, to selectively engage either lockset. A disengagement device, for preventing forces from being applied to the lock bolts is also disclosed. In this manner, a secured space can be controlled from the secured side as a safe-haven. 4 figures.

  5. Haven, Kansas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Haven, Kansas: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 37.8989003, -97.7828272 Show Map Loading map... "minzoom":false,"mappingservice"...

  6. City of Moore Haven, Florida (Utility Company) | Open Energy...

    Open Energy Info (EERE)

    Moore Haven, Florida (Utility Company) Jump to: navigation, search Name: Moore Haven City of Place: Florida Phone Number: 863-946-0711 Website: www.moorehaven.org Outage Hotline:...

  7. City of Grand Haven, Michigan (Utility Company) | Open Energy...

    Open Energy Info (EERE)

    Grand Haven Place: Michigan Phone Number: (616) 846-6250 Website: www.ghblp.org Twitter: @ghblp Facebook: https:www.facebook.comGrandHavenBLP Outage Hotline: (616) 842-2241...

  8. New Haven County, Connecticut: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Connecticut North Branford, Connecticut North Haven, Connecticut Orange, Connecticut Oxford, Connecticut Prospect, Connecticut Seymour, Connecticut Southbury, Connecticut...

  9. DOE - Office of Legacy Management -- Bridgeport Brass Co - Havens

    Office of Legacy Management (LM)

    Laboratory - CT 06 Bridgeport Brass Co - Havens Laboratory - CT 06 FUSRAP Considered Sites Site: Bridgeport Brass Co., Havens Laboratory (CT.06) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Havens Plant Havens Laboratory Reactive Metals, Inc. CT.06-1 CT.06-2 Location: 30 Grand Street and Kossuth and Pulaski Streets , Bridgeport , Connecticut CT.06-3 Evaluation Year: 1987 CT.06-4 Site Operations: From 1953 to 1962, conducted research on

  10. Veteran's Affairs Health Care System, West Haven, Connecticut | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Veteran's Affairs Health Care System, West Haven, Connecticut Veteran's Affairs Health Care System, West Haven, Connecticut Overview The West Haven (Connecticut) Campus of the Veterans Affairs Connecticut Health Care System was the first Veteran's Hospital to award a shared energy savings (SES) contract (now known as energy savings performance contracts). The project involves replacement of the lighting system, installation of a cooling system, maintenance of the new chiller

  11. Green Haven, Maryland: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Haven, Maryland: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.1395536, -76.5477413 Show Map Loading map... "minzoom":false,"mappingservic...

  12. West Haven, Vermont: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    "alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":"" Hide Map West Haven is a town in Rutland County, Vermont. It falls under Vermont's At-large...

  13. New Haven, Connecticut: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    New Haven, Connecticut: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.3081527, -72.9281577 Show Map Loading map... "minzoom":false,"mappin...

  14. West Haven-Sylvan, Oregon: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Hide Map This article is a stub. You can help OpenEI by expanding it. West Haven-Sylvan is a census-designated place in Washington County, Oregon.1 References...

  15. Wild Rose Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    Wild Rose Geothermal Area Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Wild Rose Geothermal Area Contents 1 Area Overview 2 History and Infrastructure 3 Regulatory...

  16. EIS-0445: American Electric Power Service Corporation's Mountaineer Commercial Scale Carbon Capture and Storage Demonstration, New Haven, Mason County, West Virginia

    Broader source: Energy.gov [DOE]

    DOE evaluates the potential environmental impacts of providing financial assistance for the construction and operation of a project proposed by American Electric Power Service Corporation (AEP). DOE selected tbis project for an award of financial assistance through a competitive process under the Clean Coal Power Initiative (CCPI) Program. AEP's Mountaineer Commercial Scale Carbon Capture and Storage Project (Mountaineer CCS II Project) would construct a commercial scale carbon dioxide (C02l capture and storage (CCS) system at AEP's existing Mountaineer Power Plant and other AEP owned properties located near New Haven, West Virginia.

  17. WILD PIG ATTACKS ON HUMANS

    SciTech Connect (OSTI)

    Mayer, J.

    2013-04-12

    Attacks on humans by wild pigs (Sus scrofa) have been documented since ancient times. However, studies characterizing these incidents are lacking. In an effort to better understand this phenomenon, information was collected from 412 wild pig attacks on humans. Similar to studies of large predator attacks on humans, data came from a variety of sources. The various attacks compiled occurred in seven zoogeographic realms. Most attacks occurred within the species native range, and specifically in rural areas. The occurrence was highest during the winter months and daylight hours. Most happened under non-hunting circumstances and appeared to be unprovoked. Wounded animals were the chief cause of these attacks in hunting situations. The animals involved were typically solitary, male and large in size. The fate of the wild pigs involved in these attacks varied depending upon the circumstances, however, most escaped uninjured. Most human victims were adult males traveling on foot and alone. The most frequent outcome for these victims was physical contact/mauling. The severity of resulting injuries ranged from minor to fatal. Most of the mauled victims had injuries to only one part of their bodies, with legs/feet being the most frequent body part injured. Injuries were primarily in the form of lacerations and punctures. Fatalities were typically due to blood loss. In some cases, serious infections or toxemia resulted from the injuries. Other species (i.e., pets and livestock) were also accompanying some of the humans during these attacks. The fates of these animals varied from escaping uninjured to being killed. Frequency data on both non-hunting and hunting incidents of wild pig attacks on humans at the Savannah River Site, South Carolina, showed quantitatively that such incidents are rare.

  18. NREL: Wind-Wildlife Impacts Literature Database (WILD) Home Page

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL - National Renewable Energy Laboratory Wind-Wildlife Impacts Literature Database (WILD) Wind Research WILD WILD Browse By Reset All Geography Africa (45) Apply Africa filter...

  19. Hog wild | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hog wild Hog wild Posted: May 7, 2014 - 5:28pm | Y-12 Report | Volume 10, Issue 2 | 2014 Tennessee inventor Jake Gish, winner of the third annual Y-12 Innovation Competition, has it out for feral hogs, and with good reason. Originally found across much of Europe and Asia, the beasts, which are the size of small bears, have wreaked havoc on humans since at least the Middle Ages. The wild boars have since expanded their territory and in the past few decades have become increasingly prevalent - and

  20. Interagency Wild and Scenic Rivers Coordinating Council's WSRA...

    Open Energy Info (EERE)

    to use the flowcharts created by the Interagency Wild and Scenic Rivers Coordinating Council. Author The Interagency Wild and Scenic Rivers Coordinating Council Published The...

  1. Wild Horse Wind Power Project | Open Energy Information

    Open Energy Info (EERE)

    Wind Power Project Jump to: navigation, search Name Wild Horse Wind Power Project Facility Wild Horse Wind Power Project Sector Wind energy Facility Type Commercial Scale Wind...

  2. Wild and Scenic Rivers Act (1968)

    Broader source: Energy.gov [DOE]

    The National Wild and Scenic Rivers System was created by Congress in 1968 (Public Law 90-542; 16 U.S.C. 1271 et seq.) to preserve certain rivers with outstanding natural, cultural, and recreational values in a free-flowing condition for the enjoyment of present and future generations.

  3. Wild Horse II Wind Farm | Open Energy Information

    Open Energy Info (EERE)

    II Wind Farm Jump to: navigation, search Name Wild Horse II Wind Farm Facility Wild Horse II Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner...

  4. DOE - NNSA/NFO -- Photo Library Wild Flowers

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wild Flowers NNSA/NFO Language Options U.S. DOE/NNSA - Nevada Field Office Photo Library - Wild Flowers Wild flowers such as Cane Spring suncup and White bearpoppy are included among the 750 varieties of plants located on the Nevada National Security Site. Instructions: Click the photograph THUMBNAIL to view the photograph details Thumbnail Category http://www.nv.doe.gov/library/photos/thumbs/CaneSpringsuncup.jpg Wild Flowers http://www.nv.doe.gov/library/photos/thumbs/Clokeys.jpg Wild Flowers

  5. Wild and Scenic Rivers Act | Open Energy Information

    Open Energy Info (EERE)

    as wild, scenic, or recreational. These classifications necessitate differing levels of environmental protection under the Act. Published NA Year Signed or Took Effect 1968...

  6. V-143: Fresh Java issues being exploited in the wild

    Broader source: Energy.gov [DOE]

    Java issues are being exploited in the wild by exploit kits, with Cool and Redkit specifically being known to use these bugs, and others likely to follow shortly.

  7. Technical Report of the Wild and Scenic Rivers Coordinating Council...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: Technical Report of the Wild and Scenic Rivers Coordinating...

  8. The antitrust wild card and electricity restructuring

    SciTech Connect (OSTI)

    Adelberg, A.W.; Ongman, J.W.

    1997-03-01

    If competitive policy issues in electricity restructuring are not addressed soon--preferably in federal legislation--it`s likely that someone will use the antitrust wild card to achieve its ends. Experience teaches that this may not be the best way to make public policy. As the electric utility industry restructures, it is widely assumed that Congress, the state legislatures, and regulators will set the ground rules for the restructured markets. Experience to date would seem to confirm this view: California`s restructuring legislation, FERC`s Order 888, and the restructuring proceedings in numerous states are all examples. And yet, there remains another player whose role could be equally important: The federal judiciary. While court decisions under the antitrust laws have had little influence to date on the industry`s direction, there is reason to believe that their role could increase dramatically. Certainly this is the history of other industries that have undergone similar transformations. The authors expect that forces at work in the electric utility industry could lead to antitrust actions playing a far greater role in the industry`s future than most observers currently expect. The electric utility industry has already experienced a close brush with the potential for antitrust rulings to unravel critical elements of regulatory policy on restructuring. The DC Circuit`s now famous (or infamous) dicta in the Cajun Electric Power case illustrated how a simple antitrust principle--the prohibition on so-called tying arrangements--could defeat the FERC`s policy with respect to utilities` recovery of billions of dollars of stranded costs. The FERC rebutted that dicta in its remand decisions and elsewhere, and it appears that the issue in now moot in the Cajun litigation itself. But the tying arrangement argument is far from dead.

  9. COLLOQUIUM: A Wild Solution for Climate Change | Princeton Plasma...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    15, 2015, 2:00pm to 3:30pm Colloquia MBG Auditorium COLLOQUIUM: A Wild Solution for Climate Change Professor Thomas E. Lovejoy George Mason University Presentation: PDF icon...

  10. Chemical analysis of Wild-2 samples returned by Stardust (Conference) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Conference: Chemical analysis of Wild-2 samples returned by Stardust Citation Details In-Document Search Title: Chemical analysis of Wild-2 samples returned by Stardust Authors: Flynn, G.J. ; Borg, J. ; Bleuet, P. ; Brenker , F. ; Brennan, S. ; Daghlian, C. ; Djouadi, Z. [1] ; ESRF) [2] + Show Author Affiliations (SUNYP) ( Publication Date: 2016-01-15 OSTI Identifier: 1235455 Resource Type: Conference Resource Relation: Conference: Lunar and Plnetary Science XXXVII;March

  11. WILD PIGS: BIOLOGY, DAMAGE, CONTROL TECHINQUES AND MANAGEMENT

    SciTech Connect (OSTI)

    Mayer, John; Brisbin, I. Lehr

    2009-12-31

    The existence of problems with wild pigs (Sus scrofa) is nothing new to the Western Hemisphere. Damage by these introduced animals was reported as far back as 1505 by the early Spanish colonies in the Caribbean, where wild pigs were killing the colonists cattle. Droves of these animals also ravaged cultivated crops of maize and sugarcane on islands in the West Indies during this same time period. These wild pigs reportedly were very aggressive and often attacked Spanish soldiers hunting rebellious Indians or escaped slaves on these islands, especially when these animals were cornered. The documentation of such impacts by introduced populations of this species in the United States has subsequently increased in recent years, and continued up through the present (Towne and Wentworth. 1950, Wood and Barrett 1979, Mayer and Brisbin 1991, Dickson et al. 2001). In spite of a fairly constant history in this country since the early 1900s, wild pigs have had a dramatic recent increase in both distribution and numbers in the United States. Between 1989 and 2009, the number of states reporting the presence of introduced wild pigs went from 19 up to as many as 44. This increase, in part natural, but largely manmade, has caused an increased workload and cost for land and resource managers in areas where these new populations are found. This is the direct result of the damage that these introduced animals do. The cost of both these impacts and control efforts has been estimated to exceed a billion dollars annually (Pimentel 2007). The complexity of this problem has been further complicated by the widespread appeal and economic potential of these animals as a big game species (Tisdell 1982, Degner 1989). Wild pigs are a controversial problem that is not going away and will likely only get worse with time. Not only do they cause damage, but wild pigs are also survivors. They reproduce at a rate faster than any other mammal of comparable size, native or introduced; they can eat just about anything; and, they can live just about anywhere. On top of that, wild pigs are both very difficult to control and, with the possible exception of island ecosystems, almost impossible to eradicate (Dickson et al. 2001, Sweeney et al. 2003). The solution to the wild pig problem has not been readily apparent. The ultimate answer as to how to control these animals has not been found to date. In many ways, wild pigs are America's most successful large invasive species. All of which means that wild pigs are a veritable nightmare for land and resource managers trying to keep the numbers of these animals and the damage that they do under control. Since the more that one knows about an invasive species, the easier it is to deal with and hopefully control. For wild pigs then, it is better to 'know thy enemy' than to not, especially if one expects to be able to successfully control them. In an effort to better 'know thy enemy,' a two-day symposium was held in Augusta, Georgia, on April 21-22, 2004. This symposium was organized and sponsored by U.S.D.A. Forest Service-Savannah River (USFS-SR), U. S. Department of Energy-Savannah River Operations Office (DOE-SR), the Westinghouse Savannah River Company (WSRC), the South Carolina Chapter of the Soil & Water Conservation Society, and the Savannah River Ecology Laboratory (SREL). The goal of this symposium was to assemble researchers and land managers to first address various aspects of the biology and damage of wild pigs, and then review the control techniques and management of this invasive species. The result would then be a collected synopsis of what is known about wild pigs in the United States. Although the focus of the symposium was primarily directed toward federal agencies, presenters also included professionals from academic institutions, and private-sector control contractors and land managers. Most of the organizations associated with implementing this symposium were affiliated with the Savannah River Site (SRS), a 803 km{sup 2} federal nuclear facility, located in western South Carolina along the Savannah River. The SRS was a very appropriate facility to host this symposium. The SRS has been dealing with its wild pig problem since the early 1950s. A lot has been learned about these animals at the site over the ensuing decades. Between the USFS-SR, DOE-SR, SREL, and the site's management and operations contractor, which is currently Savannah River Nuclear Solutions, SRS organizations have conducted a wealth of research on this wild pig population, spanning a broad spectrum of topics and disciplines. In fact, the SRS wild pig population is among the most studied, and possibly is the best studied population of this invasive species found in the United States today. Unfortunately, with all of that work, the ultimate answer to controlling wild pigs and their impacts still has not been found. This volume represents the collected synopsis that was the goal of the aforementioned symposium.

  12. Wind-Wildlife Impacts Literature Database (WILD)(Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2015-01-01

    The Wind-Wildlife Impacts Literature Database (WILD), developed and maintained by the National Wind Technology Center (NWTC) at the National Renewable Energy Laboratory (NREL), is comprised of over 1,000 citations pertaining to the effects of land-based wind, offshore wind, marine and hydrokinetic, power lines, and communication and television towers on wildlife.

  13. Wind-Wildlife Impacts Literature Database (WILD)(Fact Sheet), NREL (National Renewable Energy Laboratory)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wind-Wildlife Impacts Literature Database (WILD) What is WILD? The Wind-Wildlife Impacts Literature Database (WILD), developed and main- tained by the National Wind Technology Center (NWTC) at the National Renewable Energy Laboratory (NREL), is comprised of over 1,000 citations pertaining to the effects of land-based wind, offshore wind, marine and hydrokinetic power systems, power lines, and communication and television towers on wildlife. For the wind energy sector, WILD serves as an

  14. Transgenic crops get a test in the wild

    SciTech Connect (OSTI)

    Cherfas, J.

    1991-02-22

    A novel British research program called PROSAMO - Planned Release of Selected and Modified Organisms - has just produced its first batch of results on the ecological behavior of a genetically manipulated variety of oil seed rape (known to Americans as canola). As expected, the preliminary data indicate that these plants do not outgrow their competitors in the wild, nor is there any evidence that they pass on their foreign genes to other species. PROSAMO is moving on to test other crops with other foreign genes. If these results are as reassuring, scientists around the world will have solid evidence with which to soothe fears.

  15. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print Wednesday, 30 May 2007 00:00 NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination

  16. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wild 2 Viewed by ALS Microscopes Print NASA's 200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81PWild...

  17. WAC - 232-12-064 Live Wildlife-Taking from the wild | Open Energy...

    Open Energy Info (EERE)

    32-12-064 Live Wildlife-Taking from the wild Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 232-12-064 Live...

  18. Title 36 CFR 297 Wild and Scenic Rivers | Open Energy Information

    Open Energy Info (EERE)

    Abstract Section 7 of the Wild and Scenic Rivers Act provides for the protection of the free-flowing, scenic, and natural values of rivers designated as components or potential...

  19. Title 36 C.F.R. 297 Wild and Scenic Rivers | Open Energy Information

    Open Energy Info (EERE)

    Abstract Section 7 of the Wild and Scenic Rivers Act provides for the protection of the free-flowing, scenic, and natural values of rivers designated as components or potential...

  20. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  1. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  2. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  3. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  4. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  5. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  6. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  7. Dosage compensation can buffer copy-number variation in wild yeast (Journal

    Office of Scientific and Technical Information (OSTI)

    Article) | DOE PAGES Dosage compensation can buffer copy-number variation in wild yeast Title: Dosage compensation can buffer copy-number variation in wild yeast Authors: None, None Publication Date: 2015-05-08 OSTI Identifier: 1227971 Grant/Contract Number: FC02-07ER64494 Type: Published Article Journal Name: eLife Additional Journal Information: Journal Volume: 4; Journal ID: ISSN 2050-084X Publisher: eLife Sciences Publications, Ltd. Sponsoring Org: USDOE Country of Publication: United

  8. Dosage compensation can buffer copy-number variation in wild yeast (Journal

    Office of Scientific and Technical Information (OSTI)

    Article) | SciTech Connect Dosage compensation can buffer copy-number variation in wild yeast Citation Details In-Document Search Title: Dosage compensation can buffer copy-number variation in wild yeast Authors: None, None Publication Date: 2015-05-08 OSTI Identifier: 1227971 Grant/Contract Number: FC02-07ER64494 Type: Published Article Journal Name: eLife Additional Journal Information: Journal Volume: 4; Journal ID: ISSN 2050-084X Publisher: eLife Sciences Publications, Ltd. Sponsoring

  9. North Haven, Maine: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 44.1281362, -68.8741989 Show Map Loading map... "minzoom":false,"mappingservice":"googlemaps3","type"...

  10. City of Haven, Kansas (Utility Company) | Open Energy Information

    Open Energy Info (EERE)

    861 Data Utility Id 8279 Utility Location Yes Ownership M NERC Location SPP NERC SPP Yes RTO SPP Yes Activity Distribution Yes This article is a stub. You can help OpenEI by...

  11. Clean Cities: Greater New Haven Clean Cities coalition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    alternative vehicle and fuel consultation and assistance to many organizations in Connecticut and outside the state, including several towns and cities, metropolitan transit...

  12. Addition of Safe Havens Increases WIPP Underground Safety

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    been installed around the IVS, with the remaining supports in process. Electrical transformers supporting the IVS have also been placed as part of the overall IVS. U.S....

  13. Ecological interactions between hatchery summer steelhead and wild Oncorhynchus mykiss in the Willamette River basin, 2014

    SciTech Connect (OSTI)

    Harnish, Ryan A.; Green, Ethan D.; Vernon, Christopher R.; Mcmichael, Geoffrey A.

    2014-12-23

    The purpose of this study was to determine the extent to which juvenile hatchery summer steelhead and wild winter steelhead overlap in space and time, to evaluate the extent of residualism among hatchery summer steelhead in the South Santiam River, and to evaluate the potential for negative ecological interactions among hatchery summer steelhead and wild winter steelhead. Because it is not possible to visually discern juvenile winter steelhead from resident rainbow trout, we treated all adipose-intact juvenile O. mykiss as one group that represented juvenile wild winter steelhead. The 2014 study objectives were to 1) estimate the proportion of hatchery summer steelhead that residualized in the South Santiam River in 2014, 2) determine the extent to which hatchery and naturally produced O. mykiss overlapped in space and time in the South Santiam River, and 3) characterize the behavioral interactions between hatchery-origin juvenile summer steelhead and naturally produced O. mykiss. We used a combination of radio telemetry and direct observations (i.e., snorkeling) to determine the potential for negative interactions between hatchery summer and wild winter steelhead juveniles in the South Santiam River. Data collected from these two independent methods indicated that a significant portion of the hatchery summer steelhead released as smolts did not rapidly emigrate from the South Santiam River in 2014. Of the 164 radio-tagged steelhead that volitionally left the hatchery, only 66 (40.2%) were detected outside of the South Santiam River. Forty-four (26.8% of 164) of the radio-tagged hatchery summer steelhead successfully emigrated to Willamette Falls. Thus, the last known location of the majority of the tagged fish (98 of 164 = 59.8%) was in the South Santiam River. Thirty-three of the tagged hatchery steelhead were detected in the South Santiam River during mobile-tracking surveys. Of those, 21 were found to be alive in the South Santiam River over three months after their release, representing a residualization rate of 12.8% (21 of 164). Snorkeling revealed considerable overlap of habitat use (in space and time) by residual hatchery steelhead and naturally produced O. mykiss in the South Santiam River. Results from our study (and others) also indicated that hatchery steelhead juveniles typically dominate interactions with naturally produced O. mykiss juveniles. The overlap in space and time, combined with the competitive advantage that residual hatchery steelhead appear to have over naturally produced O. mykiss, increases the potential for negative ecological interactions that could have population-level effects on the wild winter steelhead population of the South Santiam River.

  14. Wood-Producing Sunflower? Mining Genetic Diversity in Desert-Dwelling Wild Species (2010 JGI User Meeting)

    ScienceCinema (OSTI)

    Knapp, Steve

    2011-04-26

    Steve Knapp from Monsanto on "Wood-Producing Sunflower? Mining Genetic Diversity in Desert-Dwelling Wild Species" on March 25, 2010 at the 5th Annual DOE JGI User Meeting

  15. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

    SciTech Connect (OSTI)

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E.

    2015-11-28

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimum detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg-1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg-1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.

  16. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E.

    2015-11-28

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimummore » detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg-1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg-1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.« less

  17. Digital Mapping and Environmental Characterization of National Wild and Scenic River Systems

    SciTech Connect (OSTI)

    McManamay, Ryan A; Bosnall, Peter; Hetrick, Shelaine L; Smith, Brennan T

    2013-09-01

    Spatially accurate geospatial information is required to support decision-making regarding sustainable future hydropower development. Under a memorandum of understanding among several federal agencies, a pilot study was conducted to map a subset of National Wild and Scenic Rivers (WSRs) at a higher resolution and provide a consistent methodology for mapping WSRs across the United States and across agency jurisdictions. A subset of rivers (segments falling under the jurisdiction of the National Park Service) were mapped at a high resolution using the National Hydrography Dataset (NHD). The spatial extent and representation of river segments mapped at NHD scale were compared with the prevailing geospatial coverage mapped at a coarser scale. Accurately digitized river segments were linked to environmental attribution datasets housed within the Oak Ridge National Laboratory s National Hydropower Asset Assessment Program database to characterize the environmental context of WSR segments. The results suggest that both the spatial scale of hydrography datasets and the adherence to written policy descriptions are critical to accurately mapping WSRs. The environmental characterization provided information to deduce generalized trends in either the uniqueness or the commonness of environmental variables associated with WSRs. Although WSRs occur in a wide range of human-modified landscapes, environmental data layers suggest that they provide habitats important to terrestrial and aquatic organisms and recreation important to humans. Ultimately, the research findings herein suggest that there is a need for accurate, consistent, mapping of the National WSRs across the agencies responsible for administering each river. Geospatial applications examining potential landscape and energy development require accurate sources of information, such as data layers that portray realistic spatial representations.

  18. The Origin of Refractory Minerals in Comet 81P/Wild 2

    SciTech Connect (OSTI)

    Chi, M; Ishii, H A; Simon, S B; Bradley, J P; Dai, Z R; Joswiak, D J; Browning, N D; Matrajt, G

    2008-11-20

    Refractory Ti-bearing minerals in the calcium-, aluminium-rich inclusion (CAI) Inti, recovered from the comet 81P/Wild 2 sample, were examined using analytical (scanning) transmission electron microscopy (STEM) methods including imaging, nanodiffraction, energy dispersive spectroscopy (EDX) and electron energy loss spectroscopy (EELS). Inti fassaite (Ca(Mg,Ti,Al)(Si,Al){sub 2}O{sub 6}) was found to have a Ti{sup 3+}/Ti{sup 4+} ratio of 2.0 {+-} 0.2, consistent with fassaite in other solar system CAIs. The oxygen fugacity (log f{sub O{sub 2}}) of formation estimated from this ratio, assuming equilibration among phases at 1509K, is -19.4 {+-} 1.3. This value is near the canonical solar nebula value (-18.1 {+-} 0.3) and in close agreement with that reported for fassaite-bearing Allende CAIs (-19.8 {+-} 0.9) by other researchers using the same assumptions. Nanocrystals of osbornite (Ti(V)N), 2-40 nm in diameter, are embedded as inclusions within anorthite, spinel and diopside in Inti. Vanadium is heterogeneously distributed within some osbornite crystals. Compositions range from pure TiN to Ti{sub 0.36}V{sub 0.64}N. The possible presence of oxide and carbide in solid solution with the osbornite was evaluated. The osbornite may contain O but does not contain C. The presence of osbornite, likely a refractory early condensate, together with the other refractory minerals in Inti, indicates that the parent comet contains solids that condensed closer to the proto-sun than the distance at which the parent comet itself accreted. The estimated oxygen fugacity and the reported isotopic and chemical compositions are consistent with Inti originating in the inner solar system as opposed to it being a surviving CAI from an extrasolar source. These results provide insight for evaluating the validity of models of radial mass transport dynamics in the early solar system. The oxidation environments inferred for the Inti mineral assemblage are inconsistent with an X-wind formation scenario. In contrast, radial mixing models allowing accretion of components from different heliocentric distances can satisfy the observations from the cometary CAI Inti.

  19. A Refractory Inclusion Returned by Stardust from Comet 81P/Wild 2

    SciTech Connect (OSTI)

    Simon, S B; Joswiak, D J; Ishii, H A; Bradley, J P; Chi, M; Grossman, L; Al?on, J; Brownlee, D E; Fallon, S; Hutcheon, I D; Matrajt, G; McKeegan, K D

    2008-05-20

    Among the samples returned from comet 81P/Wild 2 by the Stardust spacecraft is a suite of particles from one impact track (Track 25) that are Ca-, Al-rich and FeO-free. We studied three particles from this track that range in size from 5.3 x 3.2 {micro}m to 15 x 10 {micro}m. Scanning and transmission electron microscopy show that they consist of very fine-grained (from {approx}0.5 to {approx}2 {micro}m) Al-rich, Ti-bearing and Ti-free clinopyroxene, Mg-Al spinel, anorthite, perovskite, and osbornite (TiN). In addition to these phases, the terminal particle, named 'Inti', also contains melilite. All of these phases, with the exception of osbornite, are common in refractory inclusions and are predicted to condense at high temperature from a gas of solar composition. Osbornite, though very rare, has also been found in meteoritic refractory inclusions, and could have formed in a region of the nebula where carbon became enriched relative to oxygen compared to solar composition. Compositions of Ti-pyroxene in Inti are similar, but not identical, to those of fassaite from Allende inclusions. Electron energy loss spectroscopy shows that Ti-rich pyroxene in Inti has Ti{sup 3+}/Ti{sup 4+} within the range of typical meteoritic fassaite, consistent with formation under reducing conditions comparable to those of a system of solar composition. Inti is {sup 16}O-rich, with {delta}{sup 18}O {approx} {delta}{sup 17}O {approx} 40{per_thousand}, like unaltered phases in refractory inclusions and refractory IDPs. With grain sizes, mineralogy, mineral chemistry, and an oxygen isotopic composition like those of refractory inclusions, we conclude that Inti is a refractory inclusion that formed in the inner solar nebula. Identification of a particle that formed in the inner Solar System among the comet samples demonstrates that there was transport of materials from the inner to the outer nebula, probably either in a bipolar outflow or by turbulence.

  20. Comparison of the oxidation state of Fe in comet 81P/Wild 2 and chondritic-porous interplanetary dust particles

    SciTech Connect (OSTI)

    Ogliore, Ryan C.; Butterworth, Anna L.; Fakra, Sirine C.; Gainsforth, Zack; Marcus, Matthew A.; Westphal, Andrew J.

    2010-07-16

    The fragile structure of chondritic-porous interplanetary dust particles (CP-IDPs) and their minimal parent-body alteration have led researchers to believe these particles originate in comets rather than asteroids where aqueous and thermal alterations have occurred. The solar elemental abundances and atmospheric entry speed of CP-IDPs also suggest a cometary origin. With the return of the Stardust samples from Jupiter-family comet 81P/Wild 2, this hypothesis can be tested. We have measured the Fe oxidation state of 15 CP-IDPs and 194 Stardust fragments using a synchrotron-based x-ray microprobe. We analyzed {approx}300 ng of Wild 2 material - three orders of magnitude more material than other analyses comparing Wild 2 and CP-IDPs. The Fe oxidation state of these two samples of material are > 2{sigma} different: the CP-IDPs are more oxidized than the Wild 2 grains. We conclude that comet Wild 2 contains material that formed at a lower oxygen fugacity than the parent-body, or parent bodies, of CP-IDPs. If all Jupiter-family comets are similar, they do not appear to be consistent with the origin of CP-IDPs. However, comets that formed from a different mix of nebular material and are more oxidized than Wild 2 could be the source of CP-IDPs.

  1. Haven't a Cue? Mapping the CUE Space as an Aid to HRA Modeling

    SciTech Connect (OSTI)

    David I Gertman; Ronald L Boring; Jacques Hugo; William Phoenix

    2012-06-01

    Advances in automation present a new modeling environment for the human reliability analysis (HRA) practitioner. Many, if not most, current day HRA methods have their origin in characterizing and quantifying human performance in analog environments where mode awareness and system status indications are potentially less comprehensive, but simpler to comprehend at a glance when compared to advanced presentation systems. The introduction of highly complex automation has the potential to lead to: decreased levels of situation awareness caused by the need for increased monitoring; confusion regarding the often non-obvious causes of automation failures, and emergent system dependencies that formerly may have been uncharacterized. Understanding the relation of incoming cues available to operators during plant upset conditions, in conjunction with operating procedures, yields insight into understanding the nature of the expected operator response in this control room environment. Static systems methods such as fault trees do not contain the appropriate temporal information or necessarily specify the relationship among cues leading to operator response. In this paper, we do not attempt to replace standard performance shaping factors commonly used in HRA nor offer a new HRA method, existing methods may suffice. In this paper we strive to enhance current understanding of the basis for operator response through a technique that can be used during the qualitative portion of the HRA analysis process. The CUE map is a means to visualize the relationship among salient cues in the control room that help influence operator response, show how the cognitive map of the operator changes as information is gained or lost, and is applicable to existing as well as advanced hybrid plants and small modular reactor designs. A brief application involving loss of condensate is presented and advantages and limitations of the modeling approach and use of the CUE map are discussed.

  2. Recovering the Elemental Composition of Comet Wild 2 Dust in Five Stardust Impact Tracks and Terminal Particles in Aerogel

    SciTech Connect (OSTI)

    Ishii, H A; Brennan, S; Bradley, J P; Luening, K; Ignatyev, K; Pianetta, P

    2007-01-04

    The elemental (non-volatile) composition of five Stardust impact tracks and terminal particles left from capture of Comet 81P/Wild 2 dust were mapped in a synchrotron x-ray scanning microprobe with full fluorescence spectra at each pixel. Because aerogel includes background levels of several elements of interest, we employ a novel 'dual threshold' approach to discriminate against background contaminants: an upper threshold, above which a spectrum contains cometary material plus aerogel and a lower threshold below which it contains only aerogel. The difference between normalized cometary-plus-background and background-only spectra is attributable to cometary material. The few spectra in between are discarded since misallocation is detrimental: cometary material incorrectly placed in the background spectrum is later subtracted from the cometary spectrum, doubling the loss of reportable cometary material. This approach improves precision of composition quantification. We present the refined whole impact track and terminal particle elemental abundances for the five impact tracks. One track shows mass increases in Cr and Mn (1.4x), Cu, As and K (2x), Zn (4x) and total mass (13%) by dual thresholds compared to a single threshold. Major elements Fe and Ni are not significantly affected. The additional Cr arises from cometary material containing little Fe. We exclude Au intermixed with cometary material because it is found to be a localized surface contaminant carried by comet dust into an impact track. The dual threshold technique can be used in other situations where elements of interest in a small sample embedded in a matrix are also present in the matrix itself.

  3. Transportation as a Means of Increasing Wild Juvenile Salmon Survival : Recovery Issues for Threatened and Endangered Snake River Salmon : Technical Report 4 of 11.

    SciTech Connect (OSTI)

    Park, Donn L.

    1993-06-01

    Smolt transportation on the Snake and Columbia Rivers has been under nearly continuous study for 25 years. Most controversy surrounds transport of spring/summer chinook, so most analyses and discussion are devoted to that species. Sockeye migrate at the same time as spring/summer chinook as do the earliest of the fall chinook. Therefore, action taken o spring/summer chinook will also affect sockeye and fall chinook. Many factors influenced transportation study results including population structure change -- the shift from nearly all wild fish to nearly all hatchery fish; new dams; the number of turbines at Snake River dams alone increased from 3 in 1968 to 24 by 1979; installation of juvenile fish pass facilities; and calamitous natural events such as the 1977 drought. All the above had negative effects on the survival of wild fish in general and on transport test results specifically, except that when smolts were transported from the upper dam their survival was not influenced by new or existing structures downstream from the transport site.

  4. Crystal Structures of Wild-type and Mutant Methicillin-resistant Staphylococcus aureus Dihydrofolate Reductase Reveal an Alternative Conformation of NADPH that may be Linked to Trimethoprim Resistance

    SciTech Connect (OSTI)

    Frey, K.; Liu, J; Lombardo, M; Bolstad, D; Wright, D; Anderson, A

    2009-01-01

    Both hospital- and community-acquired Staphylococcus aureus infections have become major health concerns in terms of morbidity, suffering and cost. Trimethoprim-sulfamethoxazole (TMP-SMZ) is an alternative treatment for methicillin-resistant S. aureus (MRSA) infections. However, TMP-resistant strains have arisen with point mutations in dihydrofolate reductase (DHFR), the target for TMP. A single point mutation, F98Y, has been shown biochemically to confer the majority of this resistance to TMP. Using a structure-based approach, we have designed a series of novel propargyl-linked DHFR inhibitors that are active against several trimethoprim-resistant enzymes. We screened this series against wild-type and mutant (F98Y) S. aureus DHFR and found that several are active against both enzymes and specifically that the meta-biphenyl class of these inhibitors is the most potent. In order to understand the structural basis of this potency, we determined eight high-resolution crystal structures: four each of the wild-type and mutant DHFR enzymes bound to various propargyl-linked DHFR inhibitors. In addition to explaining the structure-activity relationships, several of the structures reveal a novel conformation for the cofactor, NADPH. In this new conformation that is predominantly associated with the mutant enzyme, the nicotinamide ring is displaced from its conserved location and three water molecules complete a network of hydrogen bonds between the nicotinamide ring and the protein. In this new position, NADPH has reduced interactions with the inhibitor. An equilibrium between the two conformations of NADPH, implied by their occupancies in the eight crystal structures, is influenced both by the ligand and the F98Y mutation. The mutation induced equilibrium between two NADPH-binding conformations may contribute to decrease TMP binding and thus may be responsible for TMP resistance.

  5. Infrared Spectroscopy of Wild 2 Particle Hypervelocity Tracks in Stardust Aerogel: Evidence for the presence of Volatile Organics in Comet Dust

    SciTech Connect (OSTI)

    Bajt, S; Sandford, S A; Flynn, G J; Matrajt, G; Snead, C J; Westphal, A J; Bradley, J P

    2007-08-28

    Infrared spectroscopy maps of some tracks, made by cometary dust from 81P/Wild 2 impacting Stardust aerogel, reveal an interesting distribution of volatile organic material. Out of six examined tracks three show presence of volatile organic components possibly injected into the aerogel during particle impacts. When particle tracks contained excess volatile organic material, they were found to be -CH{sub 2}-rich. Off-normal particle tracks could indicate impacts by lower velocity particles that could have bounced off the Whipple shield, therefore carry off some contamination from it. However, this theory is not supported by data that show excess organic-rich material in normal and off-normal particle tracks. It is clear that the population of cometary particles impacting the Stardust aerogel collectors also include grains that contained little or none of this volatile organic component. This observation is consistent with the highly heterogeneous nature of the collected grains, as seen by a multitude of other analytical techniques. We propose that at least some of the volatile organic material might be of cometary origin based on supporting data shown in this paper. However, we also acknowledge the presence of carbon (primarily as -CH{sub 3}) in the original aerogel, which complicates interpretation of these results.

  6. Metabolic Engineering of Light and Dark Biochemical Pathways in Wild-Type and Mutant Strains of Synechocystis PCC 6803 for Maximal, 24-Hour Production of Hydrogen Gas

    SciTech Connect (OSTI)

    Ely, Roger L.; Chaplen, Frank W.R.

    2014-03-11

    This project used the cyanobacterial species Synechocystis PCC 6803 to pursue two lines of inquiry, with each line addressing one of the two main factors affecting hydrogen (H2) production in Synechocystis PCC 6803: NADPH availability and O2 sensitivity. H2 production in Synechocystis PCC 6803 requires a very high NADPH:NADP+ ratio, that is, the NADP pool must be highly reduced, which can be problematic because several metabolic pathways potentially can act to raise or lower NADPH levels. Also, though the [NiFe]-hydrogenase in PCC 6803 is constitutively expressed, it is reversibly inactivated at very low O2 concentrations. Largely because of this O2 sensitivity and the requirement for high NADPH levels, a major portion of overall H2 production occurs under anoxic conditions in the dark, supported by breakdown of glycogen or other organic substrates accumulated during photosynthesis. Also, other factors, such as N or S limitation, pH changes, presence of other substances, or deletion of particular respiratory components, can affect light or dark H2 production. Therefore, in the first line of inquiry, under a number of culture conditions with wild type (WT) Synechocystis PCC 6803 cells and a mutant with impaired type I NADPH-dehydrogenase (NDH-1) function, we used H2 production profiling and metabolic flux analysis, with and without specific inhibitors, to examine systematically the pathways involved in light and dark H2 production. Results from this work provided rational bases for metabolic engineering to maximize photobiological H2 production on a 24-hour basis. In the second line of inquiry, we used site-directed mutagenesis to create mutants with hydrogenase enzymes exhibiting greater O2 tolerance. The research addressed the following four tasks: 1. Evaluate the effects of various culture conditions (N, S, or P limitation; light/dark; pH; exogenous organic carbon) on H2 production profiles of WT cells and an NDH-1 mutant; 2. Conduct metabolic flux analyses for enhanced H2 production profiles using selected culture conditions and inhibitors of specific pathways in WT cells and an NDH-1 mutant; 3. Create Synechocystis PCC 6803 mutant strains with modified hydrogenases exhibiting increased O2 tolerance and greater H2 production; and 4. Integrate enhanced hydrogenase mutants and culture and metabolic factor studies to maximize 24-hour H2 production.

  7. Evaluation of the 1998 Predictions of the Run-Timing of Wild Migrant Yearling Chinook and Water Quality at Multiple Locations on the Snake and Columbia Rivers using CRiSP/RealTime, 1998 Technical Report.

    SciTech Connect (OSTI)

    Beer, W. Nicholas; Hayes, Joshua A.; Shaw, Pamela

    1999-07-21

    Since 1988, wild salmon have been PIT-tagged through monitoring and research programs conducted by the Columbia River fisheries agencies and Tribes. Workers at the University of Washington have used detection data at Lower Granite Dam to generate predictions of arrival distributions for various stocks at the dam. The prediction tool is known as RealTime. In 1996, RealTime predictions were linked to a downstream migration model, CRiSP.1. The composite model, known as CRiSP/RealTime, predicts the arrival distribution and fraction transported at downriver locations.

  8. Interpretation of Wild 2 Dust Fine Structure: Comparison of Stardust Aluminium Foil Craters to the Three-Dimensional Shape of Experimental Impacts by Artificial Aggregate Particles and Meteorite Powders

    SciTech Connect (OSTI)

    Kearsley, A T; Burchell, M J; Price, M C; Graham, G A; Wozniakiewicz, P J; Cole, M J; Foster, N J; Teslich, N

    2009-12-10

    New experimental results show that Stardust crater morphology is consistent with interpretation of many larger Wild 2 dust grains being aggregates, albeit most of low porosity and therefore relatively high density. The majority of large Stardust grains (i.e. those carrying most of the cometary dust mass) probably had density of 2.4 g cm{sup -3} (similar to soda-lime glass used in earlier calibration experiments) or greater, and porosity of 25% or less, akin to consolidated carbonaceous chondrite meteorites, and much lower than the 80% suggested for fractal dust aggregates. Although better size calibration is required for interpretation of the very smallest impacting grains, we suggest that aggregates could have dense components dominated by {micro}m-scale and smaller sub-grains. If porosity of the Wild 2 nucleus is high, with similar bulk density to other comets, much of the pore-space may be at a scale of tens of micrometers, between coarser, denser grains. Successful demonstration of aggregate projectile impacts in the laboratory now opens the possibility of experiments to further constrain the conditions for creation of bulbous (Type C) tracks in aerogel, which we have observed in recent shots. We are also using mixed mineral aggregates to document differential survival of pristine composition and crystalline structure in diverse fine-grained components of aggregate cometary dust analogues, impacted onto both foil and aerogel under Stardust encounter conditions.

  9. Monitoring and Evaluation of Smolt Migration in the Columbia Basin : Volume XV : Evaluation of the 2007 Predictions of the Run-Timing of Wild and Hatchery-Reared Salmon and Steelhead Smolts to Rock Island, Lower Granite, McNary, John Day, and Bonneville Dams using Program RealTime.

    SciTech Connect (OSTI)

    Griswold, Jim; Townsend, Richard L.; Skalski, John R.

    2008-12-01

    Program RealTime provided monitoring and forecasting of the 2007 inseason outmigrations via the internet for 26 PIT-tagged stocks of wild ESU Chinook salmon and steelhead to Lower Granite and/or McNary dams, one PIT-tagged hatchery-reared ESU of sockeye salmon to Lower Granite Dam, one PIT-tagged wild stock of sockeye salmon to McNary Dam, and 20 passage-indexed runs-at-large, five each to Rock Island, McNary, John Day, and Bonneville dams. Nineteen stocks are of wild yearling Chinook salmon which were captured, PIT-tagged, and released at sites above Lower Granite Dam in 2007 and have at least one year's historical migration data previous to the 2007 migration. These stocks originate in 19 tributaries of the Salmon, Grande Ronde and Clearwater Rivers, all tributaries to the Snake River, and are subsequently detected through tag identification and monitored at Lower Granite Dam. Seven wild PIT-tagged runs-at-large of Snake or Upper Columbia River ESU salmon and steelhead were monitored at McNary Dam. Three wild PIT-tagged runs-at-large were monitored at Lower Granite Dam, consisting of the yearling and subyearling Chinook salmon and the steelhead runs. The hatchery-reared PIT-tagged sockeye salmon stock from Redfish Lake was monitored outmigrating through Lower Granite Dam. Passage-indexed stocks (stocks monitored by FPC passage indices) included combined wild and hatchery runs-at-large of subyearling and yearling Chinook, coho, and sockeye salmon, and steelhead forecasted to Rock Island, McNary, John Day, and Bonneville dams.

  10. Insulin-Like Growth Factor-Type 1 Receptor Inhibitor NVP-AEW541 Enhances Radiosensitivity of PTEN Wild-Type but Not PTEN-Deficient Human Prostate Cancer Cells

    SciTech Connect (OSTI)

    Isebaert, Sofie F.; Swinnen, Johannes V.; McBride, William H.; Haustermans, Karin M.

    2011-09-01

    Purpose: During the past decade, many clinical trials with both monoclonal antibodies and small molecules that target the insulin-like growth factor-type 1 receptor (IGF-1R) have been launched. Despite the important role of IGF-1R signaling in radioresistance, studies of such agents in combination with radiotherapy are lagging behind. Therefore, the aim of this study was to investigate the effect of the small molecule IGF-1R kinase inhibitor NVP-AEW541 on the intrinsic radioresistance of prostate cancer cells. Methods and Materials: The effect of NVP-AEW541 on cell proliferation, cell viability, IGF-1R signaling, radiosensitivity, cell cycle distribution, and double strand break repair was determined in three human prostate cancer cell lines (PC3, DU145, 22Rv1). Moreover, the importance of the PTEN pathway status was explored by means of transfection experiments with constitutively active Akt or inactive kinase-dead Akt. Results: NVP-AEW541 inhibited cell proliferation and decreased cell viability in a time-and dose-dependent manner in all three cell lines. Radiosensitization was observed in the PTEN wild-type cell lines DU145 and 22Rv1 but not in the PTEN-deficient PC3 cell line. NVP-AEW541-induced radiosensitization coincided with downregulation of phospho-Akt levels and high levels of residual double strand breaks. The importance of PTEN status in the radiosensitization effect was confirmed by transfection experiments with constitutively active Akt or inactive kinase-dead Akt. Conclusions: NVP-AEW541 enhances the effect of ionizing radiation in PTEN wild-type, but not in PTEN-deficient, prostate cancer cells. Proper patient selection based on the PTEN status of the tumor will be critical to the achievement of optimal results in clinical trials in which the combination of radiotherapy and this IGF-1R inhibitor is being explored.

  11. Monitoring and Evaluation of Smolt Migration in the Columbia Basin, Volume XIV; Evaluation of 2006 Prediction of the Run-Timing of Wild and Hatchery-Reared Salmon and Steelhead at Rock Island, Lower Granite, McNary, John Day and Bonneville Dams using Program Real Time, Technical Report 2006.

    SciTech Connect (OSTI)

    Griswold, Jim

    2007-01-01

    Program RealTime provided monitoring and forecasting of the 2006 inseason outmigrations via the internet for 32 PIT-tagged stocks of wild ESU chinook salmon and steelhead to Lower Granite and/or McNary dams, one PIT-tagged hatchery-reared ESU of sockeye salmon to Lower Granite Dam, and 20 passage-indexed runs-at-large, five each to Rock Island, McNary, John Day, and Bonneville Dams. Twenty-four stocks are of wild yearling chinook salmon which were captured, PIT-tagged, and released at sites above Lower Granite Dam in 2006, and have at least one year's historical migration data previous to the 2006 migration. These stocks originate in drainages of the Salmon, Grande Ronde and Clearwater Rivers, all tributaries to the Snake River, and are subsequently detected through the tag identification and monitored at Lower Granite Dam. In addition, seven wild PIT-tagged runs-at-large of Snake or Upper Columbia River ESU salmon and steelhead were monitored at McNary Dam. Three wild PIT-tagged runs-at-large were monitored at Lower Granite Dam, consisting of the yearling and subyearling chinook salmon and the steelhead trout runs. The hatchery-reared PIT-tagged sockeye salmon stock from Redfish Lake was monitored outmigrating through Lower Granite Dam. Passage-indexed stocks (stocks monitored by FPC passage indices) included combined wild and hatchery runs-at-large of subyearling and yearling chinook, coho, and sockeye salmon, and steelhead trout forecasted to Rock Island, McNary, John Day, and Bonneville Dams.

  12. Wild Fire Computer Model Helps Firefighters

    ScienceCinema (OSTI)

    Canfield, Jesse

    2014-06-02

    A high-tech computer model called HIGRAD/FIRETEC, the cornerstone of a collaborative effort between U.S. Forest Service Rocky Mountain Research Station and Los Alamos National Laboratory, provides insights that are essential for front-line fire fighters. The science team is looking into levels of bark beetle-induced conditions that lead to drastic changes in fire behavior and how variable or erratic the behavior is likely to be.

  13. Wild and Scenic Rivers | Open Energy Information

    Open Energy Info (EERE)

    Scenic Rivers Jump to: navigation, search Retrieved from "http:en.openei.orgwindex.php?titleWildandScenicRivers&oldid612228" Feedback Contact needs updating Image...

  14. untitled

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Terminal Woodbridge, NJ 1,000 Williams Energy Services New Haven, CT 500 Motiva Enterprises LLC New Haven, CT 250 Motiva Enterprises LLC Providence, RI 250 Source: Energy...

  15. Science on Saturday: The Wild and Wacky World of Epigenetics...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    at PPPL, adult visitors must show a government-issued photo I.D. - for example, a passport or a driver's license. Non-U.S. citizens must show a government-issued photo I.D.,...

  16. Dosage compensation can buffer copy-number variation in wild...

    Office of Scientific and Technical Information (OSTI)

    Publisher: eLife Sciences Publications, Ltd. Sponsoring Org: USDOE Country of Publication: United States Language: English Word Cloud More Like This Free Publicly Accessible Full ...

  17. Taming the Wild World of Management, Performance and Communication - 13459

    SciTech Connect (OSTI)

    Ford, Laurie

    2013-07-01

    Management has evolved a long way from its original meaning of 'governing a horse'. The industrial revolution fostered 'scientific management'; 1930's Hawthorne studies discovered that people's social interactions could alter productivity; and the dawn of the computer age in the post-war 1950's brought general systems theory into management thinking. Today, mobile wireless connectivity aims to transform ever-changing networks of players, mandates, and markets into something that can be 'managed'. So why is there no clear and simple recipe for how to practice management? We talk about financial management, safety management, and operations management, but surely the 'management' part of those endeavors will share the same set of practices. Instead, we are still arguing for 'management' to include everything from developing people to negotiating contracts. A manager's job may include many things, but one of them, the job of management, needs to be nailed down. Three standard practices for managing in a network are developed: (a) support the dialogues that connect people vital to accomplishing a goal or objective; (b) develop and sustain the scoreboards that serve as a road-map to reach the goal; and (c) control the feedback to 'govern the horse'. These three practices are useful for more than reaching goals, as they also support coordinating across boundaries and running productive meetings. The dialogues for productive relationships, scoreboards for goals and deliverables, and feedback for performance together constitute a recipe for managing in a networked world. (authors)

  18. CX-003854: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    University of New Haven Solar Testing and Training Lab (Connecticut)CX(s) Applied: B5.1Date: 09/02/2010Location(s): New Haven, ConnecticutOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  19. untitled

    Gasoline and Diesel Fuel Update (EIA)

    November 4, 2005 First Reserve Terminal Woodbridge, NJ 1,000 Williams Energy Services New Haven, CT 500 Motiva Enterprises LLC New Haven, CT 250 Motiva Enterprises LLC Providence,...

  20. untitled

    Gasoline and Diesel Fuel Update (EIA)

    December 30, 2005 First Reserve Terminal Woodbridge, NJ 1,000 Williams Energy Services New Haven, CT 500 Motiva Enterprises LLC New Haven, CT 250 Motiva Enterprises LLC Providence,...

  1. HEATRESV.CHP:Corel VENTURA

    Gasoline and Diesel Fuel Update (EIA)

    Terminal Woodbridge, NJ 1,000 Williams Energy Services New Haven, CT 500 Motiva Enterprises LLC New Haven, CT 250 Motiva Enterprises LLC Providence, RI 250 Total 2,000 Source:...

  2. Clinton County, Pennsylvania: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Flemington, Pennsylvania Lock Haven, Pennsylvania Loganton, Pennsylvania Mill Hall, Pennsylvania Renovo, Pennsylvania South Renovo, Pennsylvania Retrieved from "http:...

  3. EV Everywhere Grand Challenge- Charge to the Breakout Groups

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  4. EV Everywhere Challenge Battery Workshop

    Broader source: Energy.gov [DOE]

    Backsplash for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  5. EV Everywhere Grand Challenge - Battery Workshop Agenda

    Broader source: Energy.gov (indexed) [DOE]

    7252012 EV Everywhere Grand Challenge -- Battery Workshop Thursday, July 26, 2012 - Doubletree O'Hare, Chicago, IL Event Objective: DOE aims to obtain stakeholder input on the...

  6. EV Everywhere Battery Workshop: Setting the Stage for the EV Everywhere Grand Challenge

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge: Battery Workshop by EERE Assistant Secretary David Danielson on July 26, 2012 at the Doubletree O'Hare, Chicago, IL.

  7. HNF-54234

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cottontail (Sylvilagus nuttallii) scat, runways created or used by small mammals (rabbits or hares), and visual sightings of jackrabbits. Any significant trails or scats were...

  8. Franklin County, Missouri: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Zone Subtype A. Places in Franklin County, Missouri Berger, Missouri Gerald, Missouri Gray Summit, Missouri Leslie, Missouri Miramiguoa Park, Missouri New Haven, Missouri Oak...

  9. Sumner County, Kansas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Geuda Springs, Kansas Hunnewell, Kansas Mayfield, Kansas Milan, Kansas Mulvane, Kansas Oxford, Kansas South Haven, Kansas Wellington, Kansas Retrieved from "http:en.openei.orgw...

  10. Guilford, Connecticut: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    lt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":"" Hide Map Guilford is a town in New Haven County, Connecticut.1 Registered Energy Companies in...

  11. Waste Isolation Pilot Plant

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Havens Increases WIPP Underground Safety November 12, 2015 - WIPP Conducts Limited Scope Performance Tests October Updates October 22, 2015 - WIPP Interim Ventilation System...

  12. Jul

    Office of Scientific and Technical Information (OSTI)

    27, CH-8093 Zurich, Switzerland 21 Yale Center for Astronomy and Astrophysics, Physics Department, Yale University, PO Box 208120, New Haven, CT 06520-8120, USA 22 Kavli...

  13. ccpi-aep | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mountaineer Carbon Dioxide Capture and Storage Demonstration (Withdrawn) Mountaineer Power Plant, New Haven, West Virginia PROJECT FACT SHEET American Electric Power (AEP):...

  14. Proposal Review Panel | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Applied Physics New Haven, CT, USA Owen Duckworth (MEIS) North Carolina State University Soil Science Raleigh, NC, USA Lawrence Que, Jr. (BIO) University of Minnesota Department...

  15. Glades County, Florida: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    2 Climate Zone Subtype A. Places in Glades County, Florida Buckhead Ridge, Florida Moore Haven, Florida Retrieved from "http:en.openei.orgwindex.php?titleGladesCounty,F...

  16. Ocean County, New Jersey: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    New Jersey Manahawkin, New Jersey Mantoloking, New Jersey Mystic Island, New Jersey New Egypt, New Jersey North Beach Haven, New Jersey Ocean Acres, New Jersey Ocean Gate, New...

  17. Dunn County, Wisconsin: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Wisconsin Lucas, Wisconsin Menomonie, Wisconsin New Haven, Wisconsin Peru, Wisconsin Red Cedar, Wisconsin Ridgeland, Wisconsin Rock Creek, Wisconsin Sand Creek, Wisconsin...

  18. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... of Mechanical Engineering and Materials Science, Yale University, New Haven, ... This rapid progress of invention outpaces the development of new semiconductor ...

  19. Phoenix Press | Open Energy Information

    Open Energy Info (EERE)

    Phoenix Press Sector Wind energy Facility Type Small Scale Wind Facility Status In Service Owner Phoenix Press Energy Purchaser Phoenix Press Location New Haven CT...

  20. Rutland County, Vermont: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Vermont Clarendon, Vermont Danby, Vermont Fair Haven, Vermont Hubbardton, Vermont Ira, Vermont Killington, Vermont Mendon, Vermont Middletown Springs, Vermont Mount Holly,...

  1. Ion Source Issues for the DAE ALUS Neutrino Experiment (Conference...

    Office of Scientific and Technical Information (OSTI)

    Mark E 2 ; Havener, Charles C 2 ; Vane, C Randy 2 + Show Author Affiliations Massachusetts Institute of Technology (MIT) Massachusetts Institute of Technology (MIT) ORNL...

  2. Microsoft Word - YK 9-12-14-f.docx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 1094-701 Assistant Professor, Department of Biochemistry, Vanderbilt University,...

  3. EV Everywhere Grand Challenge - Battery Workshop attendees list

    Broader source: Energy.gov (indexed) [DOE]

    Battery Workshop Thursday, July 26, 2012 - Doubletree O'Hare, Chicago, IL Event Objective: DOE aims to obtain stakeholder input on the energy storage goals of the EV Everywhere...

  4. EV Everywhere Framing Workshop Report Out & Lessons Learned

    Broader source: Energy.gov [DOE]

    Presentation given by Vehicle Technologies Office Director Patrick Davis at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  5. EV Everywhere Framing Workshop- Report Out & Lessons Learned

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge : Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  6. A Look at MEMS Applications | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    this video on www.youtube.com, or enable JavaScript if it is disabled in your browser. hare This Article Click to email this to a friend (Opens in new window) Share on Facebook...

  7. Power Electronics and Thermal Management Breakout Session

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  8. EV Everywhere Workshop: Electric Motors and Critical Materials...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 8bpowerelectronicsed.pdf More Documents ...

  9. Shreyas Cholia! Data and Analytics Services, NERSC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Design Patterns in Web Gateways for Scientific Data --- 1 --- Joint F acili+es U ser F ... --- 2 --- Overview * Mo+va+on * PaDerns - Web D ata S haring - End---to---End F rameworks ...

  10. Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nayak, Jaladhi; Gastonguay, Adam J.; Talipov, Marat R.; Vakeel, Padmanabhan; Span, Elise A.; Kalous, Kelsey S.; Kutty, Raman G.; Jensen, Davin R.; Pokkuluri, Phani Raj; Sem, Daniel S.; et al

    2014-12-18

    Background: The mitogen-activated protein kinases (MAPKs) pathway is critical for cellular signaling, and proteins such as phosphatases that regulate this pathway are important for normal tissue development. Based on our previous work on dual specificity phosphatase-5 (DUSP5), and its role in embryonic vascular development and disease, we hypothesized that mutations in DUSP5 will affect its function. Results: In this study, we tested this hypothesis by generating full-length glutathione-S-transferase-tagged DUSP5 and serine 147 proline mutant (S147P) proteins from bacteria. Light scattering analysis, circular dichroism, enzymatic assays and molecular modeling approaches have been performed to extensively characterize the protein form and function.more » We demonstrate that both proteins are active and, interestingly, the S147P protein is hypoactive as compared to the DUSP5 WT protein in two distinct biochemical substrate assays. Furthermore, due to the novel positioning of the S147P mutation, we utilize computational modeling to reconstruct full-length DUSP5 and S147P to predict a possible mechanism for the reduced activity of S147P. Conclusion: Taken together, this is the first evidence of the generation and characterization of an active, full-length, mutant DUSP5 protein which will facilitate future structure-function and drug development-based studies.« less

  11. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81PWild 2 Viewed by ALS Microscopes Print NASA's 200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the...

  12. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    this tangle, the picture that emerged is of cometary particles containing primarily silicate materials formed within the Solar System, including some grains born in the high...

  13. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    Gasoline and Diesel Fuel Update (EIA)

    BOE Reserve Class No 2001 reserves 0.1 - 10 MBOE 10.1 - 100 MBOE 100.1 - 1,000 MBOE 1,000.1 - 10,000 MBOE 10,000.1 - 100,000 MBOE > 100,000 MBOE Basin Outline ID The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). The boundaries are not informed by subsurface structural

  14. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    Gasoline and Diesel Fuel Update (EIA)

    Gas Reserve Class No 2001 gas reserves 0.1 - 10 MMCF 10.1 - 100 MMCF 100.1 - 1,000 MMCF 1,000.1 - 10,000 MMCF 10,000.1 - 100,000 MMCF > 100,000 MMCF Basin Outline ID The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). The boundaries are not informed by subsurface

  15. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    Gasoline and Diesel Fuel Update (EIA)

    Liquids Reserve Class No 2001 liquids reserves 0.1 - 10 Mbbl 10.1 - 100 Mbbl 100.1 - 1,000 Mbbl 1,000.1 - 10,000 Mbbl 10,000.1 - 100,000 Mbbl Basin Outline ID The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). The boundaries are not informed by subsurface structural

  16. Selective progressive response of soil microbial community to wild oat roots

    SciTech Connect (OSTI)

    DeAngelis, K.M.; Brodie, E.L.; DeSantis, T.Z.; Andersen, G.L.; Lindow, S.E.; Firestone, M.K.

    2008-10-01

    Roots moving through soil enact physical and chemical changes that differentiate rhizosphere from bulk soil, and the effects of these changes on soil microorganisms have long been a topic of interest. Use of a high-density 16S rRNA microarray (PhyloChip) for bacterial and archaeal community analysis has allowed definition of the populations that respond to the root within the complex grassland soil community; this research accompanies previously reported compositional changes, including increases in chitinase and protease specific activity, cell numbers and quorum sensing signal. PhyloChip results showed a significant change in 7% of the total rhizosphere microbial community (147 of 1917 taxa); the 7% response value was confirmed by16S rRNA T-RFLP analysis. This PhyloChip-defined dynamic subset was comprised of taxa in 17 of the 44 phyla detected in all soil samples. Expected rhizosphere-competent phyla, such as Proteobacteria and Firmicutes, were well represented, as were less-well-documented rhizosphere colonizers including Actinobacteria, Verrucomicrobia and Nitrospira. Richness of Bacteroidetes and Actinobacteria decreased in soil near the root tip compared to bulk soil, but then increased in older root zones. Quantitative PCR revealed {beta}-Proteobacteria and Actinobacteria present at about 10{sup 8} copies of 16S rRNA genes g{sup -1} soil, with Nitrospira having about 10{sup 5} copies g{sup -1} soil. This report demonstrates that changes in a relatively small subset of the soil microbial community are sufficient to produce substantial changes in function in progressively more mature rhizosphere zones.

  17. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    them contributed to many of the findings that are shedding new light on how the Solar System was formed. The Stardust results show that the mixing of materials throughout the...

  18. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81PWild 2. Four ALS beamlines and the researchers using them were among the...

  19. Comet 81P/Wild 2 Under a Microscope (Journal Article) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Houston Natl. Hist. Museum, London Imperial Coll., London ESRF, Grenoble New Mexico U. Frankfurt U. SLAC Open U. UC, Davis Capodimonte Observ. Smithsonian Inst....

  20. UTILITYID","UTILNAME","STATE","YEAR","MONTH","RES_REV (Thousand...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...777,336,5848,273,2722,11152,132923 12876,"Moore Haven City of","FL",91,1,42,517,33,387,0,0...057,389,5676,216,1840,11436,135425 12876,"Moore Haven City of","FL",91,2,48,490,36,333,0,0...

  1. UTILITYID","UTILNAME","STATE","YEAR","MONTH","RES_REV (Thousand...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...207,407,6384,226,2040,11664,144814 12876,"Moore Haven City of","FL",92,1,62,605,41,387,0,0...922,418,6517,215,1828,12187,151292 12876,"Moore Haven City of","FL",92,2,51,515,41,394,0,0...

  2. UTILITYID","UTILNAME","STATE","YEAR","MONTH","RES_REV (Thousand...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...485,353,5254,250,2358,13271,155673 12876,"Moore Haven City of","FL",90,1,71,626,41,340,0,0...848,361,5418,237,2124,11176,131186 12876,"Moore Haven City of","FL",90,2,46,479,39,392,0,0...

  3. Hyman wins Fraunhofer Award

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hyman wins Fraunhofer Award Hyman wins Fraunhofer Award Jeffrey De'Haven Hyman has received the 2015 Award for Young Researchers from the International Society for Porous Media (InterPore), on behalf of the Fraunhofer Institute for Industrial Mathematics. July 14, 2015 Jeffrey De'Haven Hyman Jeffrey De'Haven Hyman Communications Office (505) 667-7000 Hyman received the award during the 2015 InterPore conference in Padova, Italy. As the award winner, he has been invited to spend approximately

  4. Cool LinksFavorite Science Questions

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    we haven't tried, that you will find the scoria scratches the tuff and not vice versa. This method is used by gemologists, who know that a rock that can be scratched by a...

  5. Milford, Connecticut: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Hide Map This article is a stub. You can help OpenEI by expanding it. Milford is a city in New Haven County, Connecticut. It falls under Connecticut's 3rd...

  6. EIS-0445: Draft Environmental Impact Statement | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    in Mason County, West Virginia near the town of New Haven. PDF icon EIS-0445-DEIS-Summary-20100.pdf PDF icon Mountaineer Commercial Scale Carbon Capture and Storage Project, Draft...

  7. Ten Things You Didn't Know About the Electron Racetrack at Brookhaven...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    We haven't spotted any baseball players back there, but deer and turkey sometimes wander in from the nearby woods. Image: Photo Courtesy of Brookhaven National Laboratory. 6 of 10 ...

  8. United Illuminating | Open Energy Information

    Open Energy Info (EERE)

    St Place: New Haven, Connecticut Zip: 06510 Region: Northeast - NY NJ CT PA Area Sector: Services Product: Green Power Marketer Website: www.uinet.com Coordinates: 41.3073289,...

  9. CX-007009: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Fuel Cell ProgramCX(s) Applied: B5.1Date: 09/22/2011Location(s): New Haven, ConnecticutOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  10. CX-009564: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    University of New Haven Solar Testing and Training Lab CX(s) Applied: B3.6 Date: 12/12/2012 Location(s): Connecticut Offices(s): Golden Field Office

  11. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    ...cy",6,29,"Leesburg","FP",,2,,913000,913000 6567,"Florida Municipal Power Agency",6,30,"Moore Haven","FP",,,,41000,41000 6567,"Florida Municipal Power Agency",6,31,"New Smyrna ...

  12. LINE","COMPNAME","COMPID","YEAR","PURCNAME","SALETYPE","MEGAWATT...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...999,"LEESBURG","FP",0,2,0,857000,857000 13,"Florida Municipal Power Agency",6567,1999,"MOORE HAVEN","FP",0,0,0,54000,54000 14,"Florida Municipal Power Agency",6567,1999,"NEW SMYRNA ...

  13. Fermilab | Visit Fermilab | Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Transportation Transportation to and from Chicago O'Hare Airport or Midway Airport is available by limousine, taxi or car rental. Transportation to and from the Geneva local commuter Metra train station on the Union Pacific West line is available by taxi or Pace Call-n-Ride. Car rental All of the usual rental companies (such as Hertz, Avis, Budget and National) are located at the airports. For the best price, we recommend Ace Rent-a-Car at O'Hare Airport, telephone 1-800-243-3443 or

  14. BPA-2013-01256-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    M1C1-Ti SSQcJTES, RC. AttojrneyN at.Law RECEIVED BY tWA FOIA OFFICE TillS DATE - 1143 DUE DATE: OF COUNS 6L 0014 5. KATES bATneonow4b. WA RuTh P HARING IL&Tri5w fri. HC'RDCZIcO Ws...

  15. Gerber-Intro.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - Newpoten:al p rojects' r equirements a dded i n s eparately * Like t o d o t he s ame f or S cratch a nd P roject s hared d isk - Please s tate 2 013 u sage a nd 2 017 n eed...

  16. Oct_2015_IM_Response_to_Public_Comments.xlsx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    e nrollment a nd p rior t o a ny S EM a ctivity, u tilities w ill r eceive a " user n ame" a nd "password" t o a ccess t he s ecure o nline E SI S ecure F ile S hare (...

  17. 01-Gerber-ASCR_Review_Overview.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    or r epos - New p rojects' r equirements a dded i n s eparately * Like t o d o t he s ame f or S cratch a nd P roject s hared d isk - Please s tate 2 013 u sage a nd 2 017 n eed...

  18. EV Everywhere Battery Workshop Introduction | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop Introduction EV Everywhere Battery Workshop Introduction Presentation given at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 1_sandalow_b.pdf More Documents & Publications EV Everywhere Framing Workshop Overview EV Everywhere Consumer Acceptance and Charging Infrastructure Workshop Introduction EA-1760: Final Environmental Assessment

  19. Fujifilm_NERSC_StorageOutlook.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - Workload c an s aturate s ystem a nd c ause i ssues f or s hared u sers - Beam l ine o r e nd s taon w ork s chedule d rives b andwidth n eeds --- 6 --- Addressing large...

  20. Significant Increase in Hydrogen Photoproduction Rates and Yields by Wild-Type Algae is Detected at High Photobioreactor Gas Phase Volume (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-07-01

    This NREL Hydrogen and Fuel Cell Technical Highlight describes how hydrogen photoproduction activity in algal cultures can be improved dramatically by increasing the gas-phase to liquid-phase volume ratio of the photobioreactor. NREL, in partnership with subcontractors from the Institute of Basic Biological Problems in Pushchino, Russia, demonstrated that the hydrogen photoproduction rate in algal cultures always decreases exponentially with increasing hydrogen partial pressure above the culture. The inhibitory effect of high hydrogen concentrations in the photobioreactor gas phase on hydrogen photoproduction by algae is significant and comparable to the effect observed with some anaerobic bacteria.

  1. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Surface Energy Budget Measurements from Nauru99 C. W. Fairall, J. E. Hare, A. A. Grachev, and A. B. White National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The National Oceanic and Atmospheric Administration (NOAA) research vessel (R/V) Ronald H. Brown conducted a series of measurements in transit to and in the vicinity of the U.S. Department of Energy (DOE) Cloud and Radiation Testbed (CART) site on Nauru in June-July 1999 as part

  2. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Examination of Island Effects on Near-Surface Bulk Meteorology and Air-Sea Fluxes from the Nauru99 Field Program C. W. Fairall and M. J. Post National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado J. E. Hare, A. B. White, and A. A. Grachev Cooperative Institute for Research in Environmental Sciences University of Colorado and National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The

  3. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Turbulent Surface Flux Measurements from Nauru99 C. W. Fairall, J. E. Hare, and A. A. Grachev National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The National Oceanic and Atmospheric Administration (NOAA) Research Vessel (R/V) Ronald H. Brown conducted a series of measurements in transit to and in the vicinity of the U.S. Department of Energy (DOE) Cloud and Radiation Testbed (CART) site on Nauru in June-July 1999 as part of a joint

  4. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Modification of the Atmospheric Boundary Layer by a Small Island: Observations from Nauru Submitter: Long, C. N., NOAA Global Monitoring Division/CIRES Area of Research: Cloud Distributions/Characterizations Working Group(s): Radiative Processes Journal Reference: Matthews, S., J. M. Hacker, J. Cole, J. Hare, C. N. Long, and R. M. Reynolds, (2007): Modification of the atmospheric boundary layer by a small island: observations from Nauru, MWR, Vol. 135, No. 3, pages 891–905. Figure 1.

  5. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    An Update on the TOGA-COARE Bulk Algorithm for Computing the Air-Sea Fluxes Fairall, C.W., and Hare, J.E., National Oceanic and Atmospheric Administration- Environmental Technology Laboratory Ninth Atmospheric Radiation Measurement (ARM) Science Team Meeting The Tropical Ocean Global Atmosphere-Coupled Ocean Atmosphere Response Experiment (TOGA-COARE) bulk algorithm version 2.5 was published in 1996. In this paper, we will provide an evaluation against a much larger data base. An improved fit

  6. Manufacturing Demonstration Facilities Workshop Agenda, March 2012

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Manufacturing Demonstration Facilities Workshop Marriott Springhill Suites O'Hare - Chicago, IL March 12, 2012 Objectives  Introduce the manufacturing community to the U.S. DOE Advanced Manufacturing Office (AMO) program vision and its goals.  Explain the proposed mechanics of the Manufacturing Demonstration Facility (MDF) concept and the objectives of this particular anticipated effort.  Encourage discussion among potential organizations that have the relevant expertise, facilities and

  7. Location | JCESR

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Location JCESR is conveniently located on the campus of the U.S. Department of Energy's Argonne National Laboratory, 25 miles southwest of Chicago at the heart of the Midwest's broad industrial and academic research and transportation communities. Visiting JCESR Argonne is easily accessible by car or public transportation from downtown Chicago, as well as from Chicago's two airports. To reach Argonne from O'Hare International Airport, take I-294 south to I-55. Exit west on I-55 (toward St.

  8. EV Everywhere - Charge to Breakout Sessions | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - Charge to Breakout Sessions EV Everywhere - Charge to Breakout Sessions Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 6_boyd_ed.pdf More Documents & Publications EV Everywhere Grand Challenge - Charge to the Breakout Groups Power Electronics and Thermal Management Breakout Session EV Everywhere EV Everywhere Grand Challenge - Electric Drive

  9. EV Everywhere Batteries Workshop - Materials Processing and Manufacturing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakout Session Report | Department of Energy Materials Processing and Manufacturing Breakout Session Report EV Everywhere Batteries Workshop - Materials Processing and Manufacturing Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. PDF icon report_out-manufacturing_b.pdf More Documents & Publications EV Everywhere Batteries Workshop - Next Generation Lithium Ion

  10. EV Everywhere Batteries Workshop - Next Generation Lithium Ion Batteries

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakout Session Report | Department of Energy Next Generation Lithium Ion Batteries Breakout Session Report EV Everywhere Batteries Workshop - Next Generation Lithium Ion Batteries Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. PDF icon report_out-next-generation_li-ion_b.pdf More Documents & Publications EV Everywhere Batteries Workshop - Beyond Lithium Ion

  11. EV Everywhere Batteries Workshop - Pack Design and Optimization Breakout

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Session Report | Department of Energy Pack Design and Optimization Breakout Session Report EV Everywhere Batteries Workshop - Pack Design and Optimization Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. PDF icon report_out-pack_design_b.pdf More Documents & Publications EV Everywhere Batteries Workshop - Beyond Lithium Ion Breakout Session Report EV Everywhere

  12. EV Everywhere Battery Workshop: Preliminary Target-Setting Framework |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Workshop: Preliminary Target-Setting Framework EV Everywhere Battery Workshop: Preliminary Target-Setting Framework Presentation given at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 4_ward_b.pdf More Documents & Publications EV Everywhere Electric Drive Workshop: Preliminary Target-Setting Framework EV Everywhere Consumer/Charging Workshop: Target-Setting Framework and Consumer Behavior EV

  13. EV Everywhere EV Everywhere Grand Challenge - Electric Drive (Power

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electronics and Electric Machines) Workshop Agenda | Department of Energy EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop Agenda EV Everywhere EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop Agenda Agenda for the EV Everywhere Grand Challenge - Electric Drive Workshop on July 24, 2012 at the Doubletree O'Hare, Chicago, IL PDF icon agenda_ed.pdf More Documents & Publications EV Everywhere

  14. EV Everywhere Electric Drive Workshop: Preliminary Target-Setting Framework

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Electric Drive Workshop: Preliminary Target-Setting Framework EV Everywhere Electric Drive Workshop: Preliminary Target-Setting Framework Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 3_ward_ed.pdf More Documents & Publications EV Everywhere Battery Workshop: Preliminary Target-Setting Framework EV Everywhere

  15. EV Everywhere Grand Challenge - Battery Status and Cost Reduction Prospects

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy Status and Cost Reduction Prospects EV Everywhere Grand Challenge - Battery Status and Cost Reduction Prospects Presentation given by technology manager David Howell at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 5_howell_b.pdf More Documents & Publications PHEV Battery Cost Assessment Overview of Battery R&D Activities Overview of Battery R&D Activities

  16. EV Everywhere Grand Challenge - Battery Workshop Agenda | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Agenda EV Everywhere Grand Challenge - Battery Workshop Agenda Agenda for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon agenda_b.pdf More Documents & Publications EV Everywhere Grand Challenge - Charge to the Breakout Groups EV Everywhere Grand Challenge Introduction for Electric Drive Workshop EV Everywhere Grand Challenge Overview Presentation

  17. EV Everywhere Grand Challenge - Battery Workshop attendees list |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy attendees list EV Everywhere Grand Challenge - Battery Workshop attendees list Attendance list for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. PDF icon companies_in_attendance_b.pdf More Documents & Publications EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop EV Everywhere Grand Challenge Overview EV Everywhere Grand Challenge Introduction for

  18. EV Everywhere Grand Challenge - Electric Drive (Power Electronics and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electric Machines) Workshop | Department of Energy - Electric Drive (Power Electronics and Electric Machines) Workshop EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop List of companies in attendance at the Electric Drive Workshop held on July 24, 2012 at the Doubletree O'Hare, Chicago, IL PDF icon companies_in_attendance_ed.pdf More Documents & Publications EV Everywhere Grand Challenge Introduction for Electric Drive Workshop EV

  19. EV Everywhere Workshop: Electric Motors and Critical Materials Breakout

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Group Report | Department of Energy Electric Motors and Critical Materials Breakout Group Report EV Everywhere Workshop: Electric Motors and Critical Materials Breakout Group Report Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 8b_power_electronics_ed.pdf More Documents & Publications EV Everywhere Workshop: Power Electronics and Thermal

  20. EV Everywhere Workshop: Power Electronics and Thermal Management Breakout

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Session Report | Department of Energy Power Electronics and Thermal Management Breakout Session Report EV Everywhere Workshop: Power Electronics and Thermal Management Breakout Session Report Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 9b_traction_drive_systems_ed.pdf More Documents & Publications EV Everywhere Batteries Workshop - Beyond

  1. EV Everywhere Workshop: Traction Drive Systems Breakout Group Report |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Traction Drive Systems Breakout Group Report EV Everywhere Workshop: Traction Drive Systems Breakout Group Report Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 7a_marlino_ed.pdf More Documents & Publications EV Everywhere - Charge to Breakout Sessions EV Everywhere Framing Workshop - Report Out & Lessons Learned

  2. Electric Drive Status and Challenges | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Status and Challenges Electric Drive Status and Challenges Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 4_rogers_ed.pdf More Documents & Publications Power Electronics and Thermal Management Breakout Session Advanced Power Electronics and Electric Motors (APEEM) R&D Program Overview Advanced Power Electronics and Electric Motors (APEEM)

  3. Electric Motors and Critical Materials | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Motors and Critical Materials Electric Motors and Critical Materials Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 7b_electric_motors-and_critical_materials_ed.pdf More Documents & Publications EV Everywhere Workshop: Power Electronics and Thermal Management Breakout Session Report EV Everywhere Workshop: Electric Motors and Critical Materials

  4. CX-100068: Categorical Exclusion Determination | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8: Categorical Exclusion Determination CX-100068: Categorical Exclusion Determination Qualifying EGS Reservoir Complexity with an Integrated Geophysical Approach-Improved Resolution Ambient Seismic Noise Interferometry Award: DE-EE0006767 CX(s) Applied: A9 Geothermal Technologies Office Date: 9/23/2014 Location(s): Nevada Office(s): Golden Field Office Board of Regents, NSHE, obo University of Nevada, Reno (hereafter UNR) would utilize DOE and cost hare funds to apply high resolution ambient

  5. Traction Drive Systems Breakout | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Traction Drive Systems Breakout Traction Drive Systems Breakout Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 9a_miller_ed.pdf More Documents & Publications Power Electronics and Thermal Management Breakout Session Electric Drive Status and Challenges Advanced Power Electronics and Electric Motors (APEEM) R&D Program Overview

  6. QA Corporate Board Meeting - June 2010 | Department of Energy

    Energy Savers [EERE]

    June 2010 QA Corporate Board Meeting - June 2010 7th EM Quality Assurance Corporate Board Meeting Meeting Location: Marriott Chicago O'Hare, 8535 West Higgins Road, Chicago, IL 60631 Room: TBD Draft Agenda for June 9, 2010 Documents Available for Download PDF icon Meeting and Presentation Materials PDF icon Meeting Minutes More Documents & Publications 2010 Quality Assurance Improvement Project Plan 2012 Quality Assurance Improvement Project Plan QA Corporate Board Meeting - Septem

  7. Wide Bandgap Semiconductors for Clean Energy Workshop Agenda

    Broader source: Energy.gov (indexed) [DOE]

    Wide Bandgap Semiconductors for Clean Energy Workshop Wednesday, July 25, 2012 Hilton Rosemont O'Hare, Chicago, IL Introduction Wide bandgap (WBG) semiconductors operate at temperatures above 150°C without external cooling, have longer lifetimes at higher operating voltages, and switch at higher frequencies with fewer power losses than today's silicon based solid-state technologies. In the same way that the invention of the silicon chip 50 years ago led to the development of the modern

  8. EV Everywhere Batteries Workshop - Beyond Lithium Ion Breakout Session

    Office of Environmental Management (EM)

    Report | Department of Energy Batteries Workshop - Beyond Lithium Ion Breakout Session Report EV Everywhere Batteries Workshop - Beyond Lithium Ion Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. PDF icon report_out-beyond_lithium_ion_b.pdf More Documents & Publications EV Everywhere Batteries Workshop - Next Generation Lithium Ion Batteries Breakout Session Report

  9. EV Everywhere Grand Challenge Introduction for Electric Drive Workshop |

    Office of Environmental Management (EM)

    Department of Energy Introduction for Electric Drive Workshop EV Everywhere Grand Challenge Introduction for Electric Drive Workshop Presentation given by EERE Assistant Secretary David Danielson at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. PDF icon 1_danielson_ed.pdf More Documents & Publications EV Everywhere Grand Challenge Overview EV Everywhere Grand Challenge

  10. Renewable Energy Loan Applications Due Today!

    Office of Energy Efficiency and Renewable Energy (EERE)

    If you haven't submitted your Part I application for Department of Energy Loan Guarantee Programs Renewables Solicitation yet, today is your last day! Round 8, Part I applications for DOE's Energy Efficiency, Renewable Energy and Advanced Transmission and Distribution Technologies Solicitation (2009) are due today by midnight EDT.

  11. Countdown to Solar Decathlon: The Info You Need Before You Go

    Broader source: Energy.gov [DOE]

    The Solar Decathlon, if you haven't heard about it, is an event put on once every two years by the U.S. Department of Energy. Essentially, 20 university teams are challenged to construct a house that is 100% powered by solar energy.

  12. bectno-180mw | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    180-MWe Demonstration of Advanced Tangentially-Fired Combustion Techniques for the Reduction of NOx Emissions from Coal-Fired Boilers - Project Brief [PDF-280KB] Southern Company Services, Inc., Lynn Haven, FL PROGRAM PUBLICATIONS Final Reports 180-MWe Demonstration of Advanced Tangentially-Fired Combustion Techniques for the Reduction of Nitrogen Oxide (NOx) Emissions from Coal-Fired Boilers, Final Report and Key Project Findings [PDF-4.6MB] (Feb 1994) CCT Reports: Project Performance

  13. PowerPoint Presentation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Klein Lawrence Livermore National Laboratory 2009 ARM Science Team Meeting Cloud Properties Working Group Breakout Monday, March 30, 2009 Cloud Data Product Priorities of the ARM Cloud Modeling Working Group Stephen A. Klein, 30 March 2009.p 2 What do cloud modelers want? * I discussed the use of cloud property retrievals by the cloud modeling working group at the November 2007 joint meeting of the Cloud Properties and Cloud Modeling Working Groups * The answers to this question haven't changed

  14. U.S. Forest Service - Biomass Activities and Tribal Projects

    Energy Savers [EERE]

    Opportunities for Partnership with the USDA Forest Service Faline Haven Office of Tribal Relations USDA Forest Service fhaven@fs.fed.us 202-205-1520 Forest Service/Tribal Relationship * "We envision a future where the Forest Service and Indian Tribes work collaboratively through government-to-government relationships to manage the resources entrusted to their care, a future where the Forest Service has the organizational structure, skills and policies to redeem our responsibilities in this

  15. AFV CoverSheet

    Office of Scientific and Technical Information (OSTI)

    299 (Accepted Manuscript) Shale gas and non-aqueous fracturing fluids: Opportunities and challenges for supercritical CO2 Middleton, Richard Stephen Carey, James William Currier, Robert Patrick Hyman, Jeffrey De'Haven Kang, Qinjun Karra, Satish Viswanathan, Hari S. Porter, Mark L. Gimenez Martinez, Joaquin Provided by the author(s) and the Los Alamos National Laboratory (2015-07-09). To be published in: Applied Energy, Vol.147, p.500-509, JUN 1 2015. DOI to publisher's version:

  16. Final Report Department of Energy Grant FG02-06ER64189

    Office of Scientific and Technical Information (OSTI)

    26 Final Report Department of Energy Grant FG02-06ER64189 Colloid-Facilitated Transport of Cations in an Unsaturated Fractured Soil under Transient Conditions by Joseph N. Ryan1, Sanjay Mohanty1, and James E. Saiers2 1 Department of Civil, Environmental, and Architectural Engineering University of Colorado Boulder UCB 428 Boulder, Colorado 80309 2 School of Forestry and Environmental Studies Yale University 195 Prospect Street New Haven, Connecticut 06511 January 31, 2015 Abstract Rainfall

  17. In the OSTI Collections: the Higgs Boson | OSTI, US Dept of Energy, Office

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Scientific and Technical Information the Higgs Boson Electromagnetism, weak interactions, and Higgs bosons Overview of the findings Further explorations New accelerator designs Uses References Additional References Research Organizations Reports Available through OSTI's SciTech Connect Data Available through OSTI's DOE Data Explorer If you flip a hundred coins and fifty-five of them come up heads, you haven't seen anything out of the ordinary. But if you flip a hundred thousand coins, and

  18. NETL F 451.1/1-1, Categorical Exclusion Designation Form

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    6952 Duke University Durham, NC Yale University - New Haven, CT; University of Kentucky - Lexington, KY; Research Triangle Institute International - Research Triangle Park, NC FE/TDIC/Coal/ETP Jason Hissam Novel Membrane and Electrodeposition-Based Separation and Recovery of Rare Earth The goal of this project is to develop and demonstrate a bench-scale technology to separate and concentrate rare earth elements from coal fly ash and other coal combustion residues. Jason C. Hissam Digitally

  19. Measured Performance of a Low Temperature Air Source Heat Pump

    SciTech Connect (OSTI)

    R.K. Johnson

    2013-09-01

    A 4-ton Low Temperature Heat Pump (LTHP) manufactured by Hallowell International was installed in a residence near New Haven, Connecticut and monitored over two winters of operation. After attending to some significant service issues, the heat pump operated as designed. This report should be considered a review of the dual compressor “boosted heat pump” technology. The Low Temperature Heat Pump system operates with four increasing levels of capacity (heat output) as the outdoor temperature drops.

  20. Hyman wins Fraunhofer Award

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hyman wins Fraunhofer Award July 14, 2015 Jeffrey De'Haven Hyman of LANL's Computational Earth Science group has received the 2015 Award for Young Researchers from the International Society for Porous Media (InterPore), on behalf of the Fraunhofer Institute for Industrial Mathematics. This award is presented to young researchers who have completed a doctorate within the past three years and who are making outstanding research contributions in the areas of porous and composite materials modeling

  1. Improving The Picture Of Nucleon Structure | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Improving The Picture Of Nucleon Structure March 1999 In a series of experiments at the Department of Energy's Thomas Jefferson National Accelerator Facility (Jefferson Lab), researchers are moving toward a substantially improved picture of nucleon structure and behavior. "There are clearly new things we see in the data," says Volker Burkert, a senior staff scientist in the Lab's Physics Division. "We see preliminary evidence for additional excited states we haven't seen before.

  2. Building America Update - March 11, 2013

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    March 11, 2013 This announcement brings you the latest information about news, activities, and publications from the U.S. Department of Energy's Building America program. Please forward this message to colleagues who may be interested in subscribing to future Building America Update newsletters. Plan to Attend the 2013 Technical Update Meeting If you haven't already registered for Building America's 2013 Technical Update Meeting scheduled for April 29-30, 2013, in Denver, Colorado, there is

  3. The SRNL Technical Assistance Program | Department of Energy

    Energy Savers [EERE]

    The SRNL Technical Assistance Program The SRNL Technical Assistance Program For over two decades, SRNL has managed a dynamic and efficient national program sponsored by DOE Office of Environmental Management that provides teams of technical experts with a broad experience base to recommend strategies to address DOE's challenging environmental problems when standard approaches haven't worked. PDF icon The SRNL Technical Assistance Program More Documents & Publications Attenuation Based

  4. Final New England Clean Power Link Project Environmental Impact Statement Appendix M Comment Response Document

    Office of Environmental Management (EM)

    (http://wamc/files/styles/default/public/201410/new-england-clean-power-link- map-ctsy-tdi-new-england.jpg" alt="">) 2. NECPL exit from Lake Champlain (Benson, Vermont) courtesy of TDI-NE 3. Lake Bomoseen, Fair Haven, Vermont courtesy of TDI-NE 4. TDI-NE 2014a 1 3 2 4 New England Clean Power Link Appendix M-Comment Response Document Department of Energy October 2015 i Table of Contents 1 Introduction

  5. DOE - Office of Legacy Management -- Yale Heavy Ion Linear Accelerator - CT

    Office of Legacy Management (LM)

    05 Yale Heavy Ion Linear Accelerator - CT 05 FUSRAP Considered Sites Site: Yale Heavy Ion Linear Accelerator (CT.05) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New Haven , Connecticut CT.05-1 Evaluation Year: 1987 CT.05-3 Site Operations: Research and development with solvents. CT.05-1 Site Disposition: Eliminated - Potential for contamination remote based on limited amount of materials handled CT.05-3 Radioactive Materials

  6. FORMERLY UTILIZED SITES REMEDIAL ACTION PROGRAM ELIMINATION REPORT

    Office of Legacy Management (LM)

    I c. ,..I -. i FORMERLY UTILIZED SITES REMEDIAL ACTION PROGRAM ELIMINATION REPORT FOR BRIDGEPORT BRASS COMPANY HAVENS LABORATORY (REACTIVE METALS, INC.) KOSSUTH AND PULASKI STREETS BRIDGEPORT, CONNECTICUT i Department of Energy Office of Nuclear Energy Office of Remedial Action and Waste Technology Division of Facility and Site Decomnissioning Projects CONTENTS INTRODUCTION BACKGROUND Site Function Site Description Radiological History and Status ELIMINATION ANALYSIS REFERENCES Page 1 . 2 ii

  7. Long-term elemental dry deposition fluxes measured around Lake Michigan with an automated dry deposition sampler

    SciTech Connect (OSTI)

    Shahin, U. Yi, S.M.; Paode, R.D.; Holsen, T.M.

    2000-05-15

    Long-term measurements of mass and elemental dry deposition (MG, Al, V, Cr, Mn, Ni, Co, Cu, Zn, As, Sr, Mo, Cd, Sb, Ba, and Pb) were made with an automated dry deposition sampler (Eagle II) containing knife-edge surrogate surfaces during the Lake Michigan Mass Balance/Mass Budget Study. Measurements were made over a roughly 700-day period in Chicago, IL; in South Haven and Sleeping Bear Dunes, MI; and over Lake Michigan on the 68th Street drinking water intake cribs from December 1993 to October 1995. Average mass fluxes in Chicago, South Haven, Sleeping Bear Dunes, and the 68th Street crib were 65, 10, 3.6, and 12 mg m{sup {minus}2} day{sup {minus}1}, respectively. Primarily crustal elemental fluxes were significantly smaller than the mass fluxes but higher than primarily anthropogenic elemental fluxes. For example, the average elemental flux of Al in Chicago, South Haven, Sleeping Bear Dunes, and the 68th Street crib were 1.0, 0.34, 0.074, and 0.34 mg m{sup {minus}2}day{sup {minus}1}, respectively. The average Pb fluxes in Chicago, South Haven, Sleeping Bear Dunes, and the 68th Street crib were 0.038, 0.023, 0.035, and 0.032 mg m{sup {minus}2}day{sup {minus}1}, respectively. The measured fluxes at the various sites were used to calculate the dry deposition loadings to the lake. These estimated fluxes were highest for Mg and lowest for Cd.

  8. 06-DataManagement-Gerhardt.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New User Training! July 15, 2014 Data Management at NERSC Where Do I Put My Data? --- 2 --- * Overview o f N ERSC fi le s ystems - Local v s. G lobal - Permanent v s. P urged * HPSS A rchive S ystem - What i s i t a nd h ow t o u se i t * Data S haring NERSC File Systems --- 3 --- The compute and storage systems 2014 Produc<on C lusters Carver, P DSF, J GI, MatComp, Planck /global/ scratch 4 PB /project 5 PB /home 250 TB 65 PB stored, 240 PB capacity, 4 0 y ears o f community d ata HPSS 16 x

  9. David Turner! NERSC User Services Group NERSC Data Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Data Management --- 1 --- September 10, 2013 Overview * File S ystem R eview, D ata S haring, D ata T ransfer - David T urner * GlobusOnline D emo - Shreyas C holia * HPSS - Lisa G erhardt * Data A nalyDcs - Yushu Y ao --- 2 --- File Systems Summary File S ystem Path Type Default Q uota Backups Purge P olicy Global H omes $HOME GPFS 40GB/1M i nodes Yes Not p urged Global S cratch $GSCRATCH GPFS 20TB/2M i nodes No 12 weeks from last a ccess Global P roject /project/ projectdirs/ projectname GPFS

  10. Microsoft Word - Document11

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    S cheinker Can s tudying i nfinite d imensional s pace h elp u s i mprove h ealthcare? Abstract: Could a hotel with a guest in each room give a new guest her own room without asking a nyone t o l eave o r s hare r ooms? C ould a s urgeon w ith a p erfect s calpel c ut a pea into 5 pieces and reassemble them into a sphere the size of the sun? Could the dough lying in a pizza pan be pushed out of the middle to form a ring of crust without being ripped? In our world, the answer to each of these

  11. Streaked x-ray spectrometer having a discrete selection of Bragg geometries

    Office of Scientific and Technical Information (OSTI)

    for Omega (Journal Article) | SciTech Connect Streaked x-ray spectrometer having a discrete selection of Bragg geometries for Omega Citation Details In-Document Search Title: Streaked x-ray spectrometer having a discrete selection of Bragg geometries for Omega The streaked x-ray spectrometer (SXS) is used with streak cameras [D. H. Kalantar, P. M. Bell, R. L. Costa, B. A. Hammel, O. L. Landen, T. J. Orzechowski, J. D. Hares, and A. K. L. Dymoke-Bradshaw, in 22nd International Congress on

  12. CMN

    Office of Legacy Management (LM)

    sl CMN - t%1UB SECRET Copy 15 MI.-396 L a SCIOTO LABORATORY MARION, OHIO ~S5_ Ct-Jt4 0 This Document Consists of 57 Pages This is Copy / o rf 23A ~S~~g.^a^~T CAMLM-396 !eo T /xY/ 'INITIALS i Contract Number AT-33-1-CEN-53 RHTG # I 1- BOX ry scIOTr LABORATORY C Operated By MONSANTO CHEMICAL COMPANY MARION, OHIO Er M. M. Haring ,u Laboratory Director rq - -- r - Er OUTLINE FOR "COLD STAND-BY" OPERATION OF SCIOTO LABORATORY * (Limited Operation and Maintenance) CAUTION This document

  13. MLM-462 Contract Number AT-33-l-GtiN-53 MOUND LABORATCRY Operatsd By

    Office of Legacy Management (LM)

    This Doeumr;t Consists of 116 ?zges This is COPY of i.3 k MLM-462 Contract Number AT-33-l-GtiN-53 MOUND LABORATCRY Operatsd By MONSAmOC~ALGoMPANY MIAMISBURG, OHIO REPORT NO. 3 OF STIEUING CaW'M'B FOR DISPUSAL CF UNITS 111 AND IV (Completion Report for Disposal of UrLt IV, Runnymeade Road sr,d Dixon live. Dayton, Ohlo) . Date: April 17, 1950 Prepared By: -36L254- F. L. Halbach Chairnan., St33ricg Gcicmittes 1ssue.d: DISTRIHJTION copy 1. - Dr. Carroll A. Hochwalt copy 2. - Dr. M. M. Haring copy 3.

  14. Oct08.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    at the site. John Schatz conveys the tale of the new site mascot, Wild Bill the kitty cat: Wild Bill came to the site about a year ago and was dubbed "Wild Bill," not because he...

  15. Energy Grasses for the Masses

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Safety Environmental Rulesets Species-Selective Harvesting Ecological Engineering Wild Harvesting System Level Implementation Multiple Platforms...

  16. CUD-55

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CUD-55 / -2 Cl - - - « « » » _ _ DB-1426 Copy No. y^ of Of > Series /f"^ ' m^ 8/9/50 This document consists of S^ pages and J figure^. COLUMBIA UNIVERSITY DEPARTMENT OF PHYSICS Contract AT-30-1-GEN-72 HIGHER RESOLUTION NEUTRON VELOCITY SPECTROMETER MEASUREMENTS OF ENRICHED URANIUM By W. W. Havens, Jr. and L. J. Rainwater, This Document Contains Restricted Data within the i'teaning of the Atcanic Energy Act of 1946 and/or information Affecting the National Defense of the United

  17. News Item

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Foundry Helps Capture Birth of Mineral in Real Time Found in seashells, pearls, marble, and chalk, calcium carbonate is one of the most important molecules on Earth. It is also the most abundant form of carbon on our planet. But while scientists have studied calcium carbonate crystal growth for decades, they haven't actually been able to explain how the crystals appear from the very start. Now, a team of researchers have used a high-powered electron microscope at the Molecular Foundry to capture

  18. Hopper Scaling Incentive Program

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hopper Scaling Incentive Program Hopper Scaling Incentive Program August 30, 2011 by Francesca Verdier For projects that haven't yet scaled their codes to 683 or more nodes (which is the level at which a job is considered "big" on hopper) NERSC is offering scaling incentives, mostly focused on the use of OpenMP. For some codes, adding OpenMP directives will allow you to scale up and run bigger science problems. For users accepted in the Scaling Incentive Program: First, you'll need to

  19. DOE Tour of Zero: Bates Avenue by Sunroc Builders | Department of Energy

    Energy Savers [EERE]

    Bates Avenue by Sunroc Builders DOE Tour of Zero: Bates Avenue by Sunroc Builders 1 of 15 Sunroc Builders built this 1,104-square-foot home in Winter Haven, Florida, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 15 ENERGY STAR-rated light-colored asphalt shingles help reflect heat, reducing solar gain during the hot weather in the Florida home. 3 of 15 The energy-efficiency features built into the compact, affordable home are expected to

  20. Featured Announcements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    August 2011 Hopper Scaling Incentive Program August 30, 2011 by Francesca Verdier For projects that haven't yet scaled their codes to 683 or more nodes (which is the level at which a job is considered "big" on hopper) NERSC is offering scaling incentives, mostly focused on the use of OpenMP. For some codes, adding OpenMP directives will allow you to scale up and run bigger science problems. For users accepted in the Scaling Incentive Program: Read the full post Intel compiler now

  1. Flying Cars | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Current Science Create a Flying Car for Daily Use? Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens in new window) Click to share on Tumblr (Opens in new window) Can Current Science Create a Flying Car for Daily Use? 2012.11.29 Chief Scientist Jim Bray explains how driving and flying differ and why flying cars haven't yet become a common method of transportation. 1 Comment Comment

  2. Clean Cities Moving Fleets Forward with Liquefied Natural Gas | Department

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Energy Cities Moving Fleets Forward with Liquefied Natural Gas Clean Cities Moving Fleets Forward with Liquefied Natural Gas May 30, 2013 - 2:52pm Addthis Waste hauler Enviro Express converted its fleet of heavy-duty trucks to run on liquefied natural gas (LNG) and built the first LNG station east of the Mississippi River with help from the Energy Department's Clean Cities initiative. | Photo courtesy of New Haven Clean Cities Coalition. Waste hauler Enviro Express converted its fleet of

  3. ARM - Status and Actions

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Status and Actions Campaign Details News Field Participants Resources Status and Actions ARM Data Discovery Browse Data Status and Actions For up-to-date campaign action, please see News 28.JUN.07 Requests for all field participants: Please place your preliminary data on the CHAPS ftp site no later than December. Connor Flynn has worked with ARM colleagues to give us a password protected FTP site. If you haven't received the instructions already from Connor regarding how to access the site,

  4. Inquiring Minds - Questions About Physics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Pure Antineutron Beams Hello, I am a physics student in Germany. I haven't had particle physics yet, so I'd be glad if you answered me one question: How do you create more or less pure anti-neutron beams in your accelerator?? I'm sure it's possible somehow but I just don't know the way to relize that. The "options" I got to know by now: collision of anti-protons with carbon nuclei can result in anti-neutrons decay of lambda-particles (how would you create them?) I guess the main

  5. Inquiring Minds - Questions About Physics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Light Theory I haven't had much time to refine, and edit this theory, so please, bear with me. Also, if the topic starts to wonder, just go with it, since my mind often wonders when I get into topics that relate to astrophysics, and atomic theory. First of, my current understanding of matter and it's behavior is that when matter hits the speed of light, 186,000 Mps, it turns into light, or energy. Now, as far as we can tell, the only matter we can get to these speeds are elementary particles.

  6. Ombuds Program

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ombuds Program Ombuds Program Committed to fair and equitable treatment of all employees, contractors, and persons doing business with the Laboratory. Contact Ombuds Office Appointments (505) 665-2837 Email Helpline (505) 667-9370 Fax (505) 667-3119 Map & Hours Hours: 8:00 a.m. - 5:00 p.m. The Ombuds Office is a safe haven. We provide a resource for people to call first when faced with conflict or disputes. Ombuds program is an independent, neutral, impartial, confidential, and informal

  7. Inquiring Minds - Questions About Physics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Why is a proton like a blueberry muffin? You Wrote: Dear Sir/Madam Are Protons Made from two up quarks and one down or do these Quarks exist inside the Proton as shown in diagrams? If they exist inside, then what is the 'shell' or 'rest' of the Proton made from? (I assume Neutrons are similar other than having one up and two down Quarks). I've heard of a neutron star but I haven't heard of a proton star is there such a thing? If not why? Andrew Russell Dear Andrew, First about proton structure:

  8. Inquiring Minds - Questions About Physics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Graviton Question I am a sophomore in high school writing a reaserch paper for my honors chemistry class. While gathering information from the local libraries one book mentioned a theoretical boson called a graviton that would be responsible for the force of gravity. Could such a particle exist or is there any evidence that it might exist? If so, are there any efforts under way to try to discover it? I haven't been able to find very much information about this subject, or maybe im just looking

  9. Fact #599: November 30, 2009 Historical Trend for Light Vehicle Sales |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 599: November 30, 2009 Historical Trend for Light Vehicle Sales Fact #599: November 30, 2009 Historical Trend for Light Vehicle Sales The sales of light vehicles dropped from 16.1 million vehicles in 2007 to 13.2 million vehicles in 2008. Light vehicle sales haven't dropped off that sharply in one year since 1974, when sales fell by 3 million vehicles from the 1973 sales level. Though the sales decline in the early 1980's was large, the decline was spread over several

  10. Simple Maintenance Saves Costly Furnace Repair/Replacement | Department of

    Energy Savers [EERE]

    Energy Simple Maintenance Saves Costly Furnace Repair/Replacement Simple Maintenance Saves Costly Furnace Repair/Replacement January 6, 2010 - 8:26am Addthis Chris Stewart Senior Communicator at DOE's National Renewable Energy Laboratory For the past few weeks, my forced-air gas furnace has been on the fritz. I blame this on the fact that I haven't been as diligent as I should have been with regular furnace maintenance, which includes: Checking the condition of the vent connection pipe and

  11. Stay Cool for Less Money with a Ceiling Fan | Department of Energy

    Energy Savers [EERE]

    Cool for Less Money with a Ceiling Fan Stay Cool for Less Money with a Ceiling Fan May 12, 2009 - 5:00am Addthis Allison Casey Senior Communicator, NREL After a few late snowstorms here in Colorado, I am more than ready to turn off the heat and enjoy some warm spring weather. We haven't had any heat waves here yet, but many of you have already been trying to stay cool; our friends in Washington, D.C. were sweltering (by comparison) in 90+°F weather just a couple short weeks ago! Whether you're

  12. DOE Tour of Zero: Bates Avenue by Sunroc Builders | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bates Avenue by Sunroc Builders DOE Tour of Zero: Bates Avenue by Sunroc Builders Addthis 1 of 15 Sunroc Builders built this 1,104-square-foot home in Winter Haven, Florida, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 15 ENERGY STAR-rated light-colored asphalt shingles help reflect heat, reducing solar gain during the hot weather in the Florida home. 3 of 15 The energy-efficiency features built into the compact, affordable home are

  13. Natural Gas | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Natural Gas Natural Gas Many heavy-duty fleets depend on diesel fuel. But an increasing number of trucking companies are transitioning their vehicles to run on liquefied natural gas (LNG), reducing fuel costs and harmful emissions in the process. <a href="/node/655801">Learn more</a> about the Energy Department's work to advance natural gas vehicle technology. | Photo courtesy of New Haven Clean Cities Coalition. Many heavy-duty fleets depend on diesel fuel. But an

  14. There's gold in garbage

    SciTech Connect (OSTI)

    Pollock, C. )

    1987-03-01

    We haven't begun to mine wealth in the world's wastes, but still insist on burying it - even while cities are running out of landfill space. Most of the products available to consumers are purchased, consumed and tossed with little regard for their remaining value. The energy, materials, and environmental losses associated with this consumption pattern are staggering. Recycling offers the opportunity to trim waste-disposal needs, simultaneously reducing disposal costs and combating global environmental problems. Recycling metals, paper, glass, plastics, and organic wastes lessens the demand for energy and materials.

  15. Have You Seen Our New Interactive Facebook Promotions? | Department of

    Office of Environmental Management (EM)

    Energy Seen Our New Interactive Facebook Promotions? Have You Seen Our New Interactive Facebook Promotions? August 4, 2011 - 6:36am Addthis If you haven't seen the Energy Savers Facebook page in a while, you'll want to check it out. The Department of Energy is partnering with the Ad Council to show consumers how easy it is to save money by saving energy, including videos, quizzes, and quick facts. Check out the new "Saving Energy Saves You Money" tab on our Facebook page! Have you

  16. DOE - Office of Legacy Management -- Olin Mathieson - CT 0-02

    Office of Legacy Management (LM)

    Olin Mathieson - CT 0-02 FUSRAP Considered Sites Site: OLIN MATHIESON (CT.0-02 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: United Nuclear Corporation CT.0-02-1 Location: New Haven , Connecticut CT.0-02-1 Evaluation Year: 1987 CT.0-02-1 Site Operations: Began fabrication of nuclear reactor fuel elements for AEC circa late-1950s. Later became part of a group forming United Nuclear Corp. and were then licensed by AEC. Performed work for U.S. Navy and

  17. DOE - Office of Legacy Management -- Seymour CT Site - CT 02

    Office of Legacy Management (LM)

    Seymour CT Site - CT 02 FUSRAP Considered Sites Seymour, CT Alternate Name(s): Bridgeport Brass Company Seymour Specialty Wire Reactive Metals, Inc. National Distillers and Chemical Co. Havens Plant CT.02-2 CT.02-3 CT.02-6 Location: 15 Franklin Street, Seymour, Connecticut CT.02-4 Historical Operations: Procured, processed and stored uranium oxides, salts, and metals for AEC and processed the products by cold-forming or extruding natural uranium metal. CT.02-3 CT.02-9 Eligibility Determination:

  18. DOE Completes Sale of Northeast Home Heating Oil Stocks | Department of

    Office of Environmental Management (EM)

    Energy Completes Sale of Northeast Home Heating Oil Stocks DOE Completes Sale of Northeast Home Heating Oil Stocks February 10, 2011 - 12:00pm Addthis Washington, DC - The U.S. Department of Energy (DOE) today has awarded contracts to four companies who successfully bid for the purchase of 1,000,000 barrels of heating oil from the Northeast Home Heating Oil Reserve storage sites in Groton and New Haven, CT. Hess Groton Terminal, Groton, CT Shell Trading U.S. Company 150,000 barrels Sprague

  19. New England Clean Power Link Project Environmental Impact Statement

    Office of Environmental Management (EM)

    Cover Photo Credits 1. TDI-NE (http://wamc/files/styles/default/public/201410/new-england-clean-power-link-map- ctsy-tdi-new-england.jpg" alt="">) 2. NECPL exit from Lake Champlain (Benson, Vermont) courtesy of TDI-NE 3. Lake Bomoseen, Fair Haven, Vermont courtesy of TDI-NE 4. TDI-NE 2014a 1 3 2 4 FINAL NEW ENGLAND CLEAN POWER LINK PROJECT ENVIRONMENTAL IMPACT STATEMENT DOE/EIS-0503 VOLUME I: IMPACT ANALYSES U.S. DEPARTMENT OF ENERGY OFFICE OF ELECTRICITY DELIVERY AND ENERGY

  20. New England Clean Power Link Project Final Environmental Impact Statement

    Office of Environmental Management (EM)

    Photo Credits 1. TDI-NE (http://wamc/files/styles/default/public/201410/new-england-clean-power-link-map-ctsy- tdi-new-england.jpg" alt="">) 2. NECPL exit from Lake Champlain (Benson, Vermont) courtesy of TDI-NE 3. Lake Bomoseen, Fair Haven, Vermont courtesy of TDI-NE 4. TDI-NE 2014a 1 3 2 4 FINAL NEW ENGLAND CLEAN POWER LINK PROJECT ENVIRONMENTAL IMPACT STATEMENT DOE/EIS-0503 VOLUME II: APPENDICES U.S. DEPARTMENT OF ENERGY OFFICE OF ELECTRICITY DELIVERY AND ENERGY RELIABILITY

  1. Department of Interior, Energy & Agriculture Collaborative BiofacilityInitiative

    Office of Environmental Management (EM)

    Interior, Energy & Agriculture Collaborative Biofacility Initiative Faline Haven Office of Tribal Relations USDA Forest Service fhaven@fs.fed.us 202-263-9396 Federal Land Management Agencies 77 million ha 106 million ha 38 million ha 34 million ha 22 million ha The National Forests and Grasslands - 77 million hectares (8% of U.S. Land Area) Ownership of Lands Managed by the Federal Government Total Federal Lands = 283 million hectares (1/3 of US land base) Forest Service/Tribal Relationship

  2. Draft New England Clean Power Link Project Environmental Impact Statement Volume I

    Office of Environmental Management (EM)

    (http://wamc/files/styles/default/public/201410/new-england-clean-power-link-map- ctsy-tdi-new-england.jpg" alt="">) 2. NECPL exit from Lake Champlain (Benson, Vermont) courtesy of TDI-NE 3. Lake Bomoseen, Fair Haven, Vermont courtesy of TDI-NE 4. TDI-NE 2014a 1 3 2 4 Department of Energy Washington, DC 20585 June 2015 Dear Sir/Madam: Enclosed for your review and comment is the New England Clean Power Link Transmission Line Project Draft Environmental Impact Statement

  3. Microsoft Word - NST, 2010d (was 2010a) potable water email David Rudolph NTS Public Water Systems description SWEISNTSWS recd

    National Nuclear Security Administration (NNSA)

    From: Michael Skougard [mailto:michael.skougard@gmail.com] Sent: Thursday, January 21, 2010 12:36 PM To: Andrea Wilkes; Stacey Schueler Cc: Brian Whipple; Mike West; Enyeart, Sandra B. Subject: Fwd: info Andrea and Stacey, I think this will answer all, or at least most, of your questions regarding NTS water systems. Ignore the square mileage number in the very first paragraph--it's the old number (I guess these guys haven't been informed that the NTS got bigger about 9 years ago!). Please let me

  4. Richard Gerber! NERSC Senior Science Advisor! User Services Group Lead

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    User Services Group Lead NUG Webinar January 2014 --- 1 --- January 9 , 2 014 Agenda * Edison C onfigura6on f or A Y 2 014 * Alloca6on Y ear R ollover I ssues * Project D irectories p er P roject * NUG 2 014 U ser G roup M ee6ng * Automa6c I /O P rofiling f or Y our C ode * Give / T ake U 6lity f or S haring F iles * NUG Q ueue C ommiRee U pdate * User Survey Needs Your Input * Open D iscussion --- 2 --- Edison Update --- 3 --- Jeff B roughton NERSC D eputy f or O pera6ons Systems D epartment H

  5. H

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    H ybrid M PI/OpenMP, UPC, a nd C AF a t N ERSC Yun ( Helen) H e a nd W oo---Sun Y ang NERSC U ser G roup M eeFng February 2 , 2 012 2 Outline * Architecture T rend * Benefits o f H ybrid M PI/OpenMP * Hybrid M PI/OpenMP P rogramming M odel * Hybrid M PI/OpenMP I ssues * Compile a nd R un h ybrid M PI/OpenMP * Using U PC a nd C AF o n H opper 3 Common A rchitectures * Shared M emory A rchitecture - MulFple C PUs s hare g lobal m emory, c ould h ave l ocal c ache - Uniform M emory A ccess ( UMA) -

  6. Title 16 U.S.C. 1278(a) Restrictions on Water Resources Projects...

    Open Energy Info (EERE)

    under the Federal Power Act on or directly affecting any river which is designated as a component of the national wild and scenic rivers system under the Wild and Scenic Rivers...

  7. Beasley Lab | Savannah River Ecology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ecology and Management of Wild Pigs SPATIAL ECOLOGY, POPULATION DYNAMICS, GENETIC STRUCTURE, AND DISEASE DYNAMICS OF WILD PIGS ON THE SAVANNAH RIVER SITE SREL Collaborators: Jim Beasley, Gene Rhodes, Shem Unger Outside Collaborators: Fred Cunningham (APHIS), Kim Pepin (APHIS), John Kilgo (USFS), Tracy Grazia (USFS), Toni Piaggio (APHIS) Wild pigs (Sus scrofa) are a highly invasive species that are rapidly expanding in number and distribution throughout the U.S. It is estimated that wild pig

  8. Stock Assessment of Columbia River Anadromous Salmonids : Final Report, Volume II, Steelhead Stock Summaries, Stock Transfer Guidelines, Information Needs.

    SciTech Connect (OSTI)

    Howell, Philip J.

    1985-07-01

    This report presents brief descriptions of wild and hatchery-raised steelhead trout stocks in the Columbia River Basin. (ACR)

  9. Advocate- Issue 47- July 2012

    Broader source: Energy.gov [DOE]

    Here are some of the topics from this issue: Wild West Tour, EM SSAB Chairs’ Meeting, and Reservation Updates.

  10. Using Experience to Train the Next Generation of Workers | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Using Experience to Train the Next Generation of Workers Using Experience to Train the Next Generation of Workers July 5, 2011 - 1:46pm Addthis Bob Wilds working at a winding machine. | Photo Courtesy of Waukesha Electric Systems Bob Wilds working at a winding machine. | Photo Courtesy of Waukesha Electric Systems Lindsey Geisler Lindsey Geisler Public Affairs Specialist, Office of Public Affairs Bob Wilds has worked at Waukesha Electric Systems for the last 12 years as a coil winder.

  11. Fifteenmile Basin Habitat Enhancement Project: Annual Report...

    Office of Scientific and Technical Information (OSTI)

    wild winter steelhead in the Fifteenmile Creek Basin under the Columbia River Basin Fish and Wildlife Program. The project is funded by through the Bonneville Power...

  12. RAPID/Roadmap/12-ID-a | Open Energy Information

    Open Energy Info (EERE)

    Contact Us State Biological Resource Considerations (12-ID-a) The Idaho Department of Fish & Game preserves wildlife against any direct take, including wild animals, birds, and...

  13. DOE Advanced Scientific Computing Advisory Subcommittee (ASCAC...

    Office of Scientific and Technical Information (OSTI)

    Paul 7 ; Debenedictus, Erik 2 ; Hiller, Jon 21 ; Kim, K. H. 7 ; Langston, Harper 15 ; Murphy, Richard Micron ; Webster, Clayton 11 ; Wild, Stefan 20 ; Grider,...

  14. Value-added agriculture offers small agribusinesses additional...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for Samuel Aragn's nine grandchildren-like the idea of supporting the northern New Mexico economy by continuing to buy fruit, including wild-harvested chokecherries, from...

  15. ch_4

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Game in reducing crop damage on adjacent private agri- cultural lands caused by wild game. INEEL is a designated National Environmental Research Park, functioning as a field...

  16. Structure of the DUF2233 Domain in Bacteria and the Stuttering...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    importance of most of these conserved residues as judged by comparison with the activity of wild-type UCE and its influence on trafficking to the Golgi. Similar...

  17. 2012_Q4

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bird banding is a technique used to study wild birds by attaching a tag to their ... processes; and use of quantitative or qualitative consideration of the full range ...

  18. HNF-56769

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dissemination Unlimited Hanford Site Raptor Nest Monitoring Report for Calendar Year 2013 J. Nugent, C. Lindsey, J. Wilde Mission Support Alliance Date Published February 2014...

  19. Approved for Public Release Further Dissemination Unlimited

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unlimited Hanford Reach Fall Chinook Redd Monitoring Report for Calendar Year 2014 J. Nugent and J. Wilde Mission Support Alliance Date Published September 2015 Prepared for...

  20. TEC Meeting Summaries - April 2004 Presentations | Department...

    Office of Environmental Management (EM)

    PDF icon Federal Agency Panel - DOTBlackwell PDF icon Federal Agency Panel - DOHLarson PDF icon Regional Group Updates on Transportation Activities - CSG-NEPaull-Wilds PDF...

  1. RAPID/Roadmap/20-FD-b | Open Energy Information

    Open Energy Info (EERE)

    on one of the following: Indian lands; Wilderness areas; Wild and scenic rivers; Wetlands; Units of the National Park System, National Refuges, or National Fish Hatcheries;...

  2. RAPID/Roadmap/7-FD-f | Open Energy Information

    Open Energy Info (EERE)

    on one of the following: Indian lands; Wilderness areas; Wild and scenic rivers; Wetlands; Units of the National Park System, National Refuges, or National Fish Hatcheries;...

  3. Secretary Chu Discovers Transformers in Wisconsin | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Bob Wilds working at a winding machine. | Photo Courtesy of Waukesha Electric Systems Using Experience to Train the Next Generation of Workers A robot works on crystalline solar ...

  4. Once nearly extinct, Idaho sockeye regaining fitness advantage

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in the wild once more. A newly published analysis by the Idaho Department of Fish and Game and the Northwest Fisheries Science Center shows endangered Snake River...

  5. Retractable barrier strip

    DOE Patents [OSTI]

    Marts, Donna J. (Idaho Falls, ID); Barker, Stacey G. (Idaho Falls, ID); Wowczuk, Andrew (Wheeling, WV); Vellenoweth, Thomas E. (Wheeling, WV)

    2002-01-01

    A portable barrier strip having retractable tire-puncture spikes for puncturing a vehicle tire. The tire-puncture spikes have an armed position for puncturing a tire and a retracted position for not puncturing a tire. The strip comprises a plurality of barrier blocks having the tire-puncture spikes removably disposed in a shaft that is rotatably disposed in each barrier block. The plurality of barrier blocks hare hingedly interconnected by complementary hinges integrally formed into the side of each barrier block which allow the strip to be rolled for easy storage and retrieval, but which prevent irregular or back bending of the strip. The shafts of adjacent barrier blocks are pivotally interconnected via a double hinged universal joint to accommodate irregularities in a roadway surface and to transmit torsional motion of the shaft from block to block. A single flexshaft cable is connected to the shaft of an end block to allow a user to selectively cause the shafts of a plurality of adjacently connected barrier blocks to rotate the tire-puncture spikes to the armed position for puncturing a vehicle tire, and to the retracted position for not puncturing the tire. The flexshaft is provided with a resiliently biased retracting mechanism, and a release latch for allowing the spikes to be quickly retracted after the intended vehicle tire is punctured.

  6. Blue light photoreceptors and methods of using the same

    DOE Patents [OSTI]

    Cashmore, Anthony Robert (Penn Valley, PA); Ahmad, Margaret (Philadelphia, PA); Lin, Chentao (Upper Darby, PA)

    1998-01-01

    The invention features a substantially pure preparation of a nucleic acid encoding a HY4 or a HY4-related gene. The invention further features transgenic plants encoding a HY4 gene having a shorter stem than substantially homozygous wild type nontransgenic plants; and, transgenic plants comprising complementary HY4 sequences having a longer stem than substantially homozygous wild type nontransgenic plants.

  7. CYP2E1-dependent elevation of serum cholesterol, triglycerides, and hepatic bile acids by isoniazid

    SciTech Connect (OSTI)

    Cheng, Jie; Krausz, Kristopher W.; Li, Feng; Ma, Xiaochao; Gonzalez, Frank J.

    2013-01-15

    Isoniazid is the first-line medication in the prevention and treatment of tuberculosis. Isoniazid is known to have a biphasic effect on the inhibitioninduction of CYP2E1 and is also considered to be involved in isoniazid-induced hepatotoxicity. However, the full extent and mechanism of involvement of CYP2E1 in isoniazid-induced hepatotoxicity remain to be thoroughly investigated. In the current study, isoniazid was administered to wild-type and Cyp2e1-null mice to investigate the potential toxicity of isoniazid in vivo. The results revealed that isoniazid caused no hepatotoxicity in wild-type and Cyp2e1-null mice, but produced elevated serum cholesterol and triglycerides, and hepatic bile acids in wild-type mice, as well as decreased abundance of free fatty acids in wild-type mice and not in Cyp2e1-null mice. Metabolomic analysis demonstrated that production of isoniazid metabolites was elevated in wild-type mice along with a higher abundance of bile acids, bile acid metabolites, carnitine and carnitine derivatives; these were not observed in Cyp2e1-null mice. In addition, the enzymes responsible for bile acid synthesis were decreased and proteins involved in bile acid transport were significantly increased in wild-type mice. Lastly, treatment of targeted isoniazid metabolites to wild-type mice led to similar changes in cholesterol, triglycerides and free fatty acids. These findings suggest that while CYP2E1 is not involved in isoniazid-induced hepatotoxicity, while an isoniazid metabolite might play a role in isoniazid-induced cholestasis through enhancement of bile acid accumulation and mitochondria ?-oxidation. -- Highlights: ? Isoniazid metabolites were elevated only in wild-type mice. ? Isoniazid caused no hepatotoxicity in wild-type and Cyp2e1-null mice. ? Isoniazid elevated serum cholesterol and triglycerides, and hepatic bile acids. ? Bile acid transporters were significantly decreased in isoniazid-treated mice.

  8. Idaho Habitat/Natural Production Monitoring Part I, 1994 Annual Report.

    SciTech Connect (OSTI)

    Hall-Griswold, Judy A.; Leitzinger, Eric J.; Petrosky, C.E. (Idaho Department of Fish and Game, Boise, ID

    1995-11-01

    A total of 333 stream sections were sampled in 1994 to monitor in chinook salmon and steelhead trout parr populations in Idaho. Percent carry capacity and density estimates were summarized by different classes of fish: wild A-run steelhead trout, wild B-run steelhead trout, natural A-run steelhead trout, natural B-run steelhead trout, wild spring and summer chinook salmon. These data were also summarized by cells and subbasins as defined in Idaho Department of Fish and Game`s 1992-1996 Anadromous Fish Management Plan.

  9. Streaked x-ray spectrometer having a discrete selection of Bragg geometries for Omega

    SciTech Connect (OSTI)

    Millecchia, M.; Regan, S. P.; Bahr, R. E.; Romanofsky, M.; Sorce, C.

    2012-10-15

    The streaked x-ray spectrometer (SXS) is used with streak cameras [D. H. Kalantar, P. M. Bell, R. L. Costa, B. A. Hammel, O. L. Landen, T. J. Orzechowski, J. D. Hares, and A. K. L. Dymoke-Bradshaw, in 22nd International Congress on High-Speed Photography and Photonics, edited by D. L. Paisley and A. M. Frank (SPIE, Bellingham, WA, 1997), Vol. 2869, p. 680] positioned with a ten-inch manipulator on OMEGA [T. R. Boehly et al., Opt. Commun. 133, 495 (1997)] and OMEGA EP [L. J. Waxer et al., Presented at CLEO/QELS 2008, San Jose, CA, 4-9 May 2008 (Paper JThB1)] for time-resolved, x-ray spectroscopy of laser-produced plasmas in the 1.4- to 20-keV photon-energy range. These experiments require measuring a portion of this photon-energy range to monitor a particular emission or absorption feature of interest. The SXS relies on a pinned mechanical reference system to create a discrete set of Bragg reflection geometries for a variety of crystals. A wide selection of spectral windows is achieved accurately and efficiently using this technique. It replaces the previous spectrometer designs that had a continuous Bragg angle adjustment and required a tedious alignment calibration procedure. The number of spectral windows needed for the SXS was determined by studying the spectral ranges selected by OMEGA users over the last decade. These selections are easily configured in the SXS using one of the 25 discrete Bragg reflection geometries and one of the six types of Bragg crystals, including two curved crystals.

  10. Mountaineer Commerical Scale Carbon Capture and Storage (CCS) Project

    SciTech Connect (OSTI)

    Deanna Gilliland; Matthew Usher

    2011-12-31

    The Final Technical documents all work performed during the award period on the Mountaineer Commercial Scale Carbon Capture & Storage project. This report presents the findings and conclusions produced as a consequence of this work. As identified in the Cooperative Agreement DE-FE0002673, AEP's objective of the Mountaineer Commercial Scale Carbon Capture and Storage (MT CCS II) project is to design, build and operate a commercial scale carbon capture and storage (CCS) system capable of treating a nominal 235 MWe slip stream of flue gas from the outlet duct of the Flue Gas Desulfurization (FGD) system at AEP's Mountaineer Power Plant (Mountaineer Plant), a 1300 MWe coal-fired generating station in New Haven, WV. The CCS system is designed to capture 90% of the CO{sub 2} from the incoming flue gas using the Alstom Chilled Ammonia Process (CAP) and compress, transport, inject and store 1.5 million tonnes per year of the captured CO{sub 2} in deep saline reservoirs. Specific Project Objectives include: (1) Achieve a minimum of 90% carbon capture efficiency during steady-state operations; (2) Demonstrate progress toward capture and storage at less than a 35% increase in cost of electricity (COE); (3) Store CO{sub 2} at a rate of 1.5 million tonnes per year in deep saline reservoirs; and (4) Demonstrate commercial technology readiness of the integrated CO{sub 2} capture and storage system.

  11. Communicating between the Apple and the Wang

    SciTech Connect (OSTI)

    Barton, G.W., Downey, R.

    1982-10-26

    This manual covers what the beginner needs to know in order to transfer files between an Apple's Microcomputer and a Wang's OIS Word Processor. We have also tried to indicate where the experienced user might want to look for additional details. We cover the use of Apple Writer )(, VisiTerm, VisiCalc, and EasyWriter Professional. For us, the two most useful connections are from Applewriter to Wang and from Visicalc to Wang. From Wang to Apple Writer via Visiterm may have some value. Files can be transferred by VisiTerm to Wang, but they arrive with RETURNS in the middle of words, which have to be edited out, so we do not recommend it. We describe how to go from EasyWriter to Wang, but we do not know how to go from Wang to EasyWriter. We see no reason to go from Wang to VisiCalc, so we haven't thought about it. All instructions are given for a typical configuration of the Apple, namely the one on which this manual was composed. It is detailed in the section on Hardware and Software.

  12. Investigation about decoupling capacitors of PMT voltage divider effects on neutron-gamma discrimination

    SciTech Connect (OSTI)

    Divani, Nazila Firoozabadi, Mohammad M.; Bayat, Esmail

    2014-11-24

    Scintillators are almost used in any nuclear laboratory. These detectors combine of scintillation materials, PMT and a voltage divider. Voltage dividers are different in resistive ladder design. But the effect of decoupling capacitors and damping resistors havent discussed yet. In this paper at first a good equilibrium circuit designed for PMT, and it was used for investigating about capacitors and resistors in much manner. Results show that decoupling capacitors have great effect on PMT output pulses. In this research, it was tried to investigate the effect of Capacitors value and places on PMT voltage divider in Neutron-Gamma discrimination capability. Therefore, the voltage divider circuit for R329-02 Hamamatsu PMT was made and Zero Cross method used for neutron-gamma discrimination. The neutron source was a 20Ci Am-Be. Anode and Dynode pulses and discrimination spectrum were saved. The results showed that the pulse height and discrimination quality change with the value and setting of capacitors.

  13. Beryllium disease

    SciTech Connect (OSTI)

    Not Available

    1991-12-20

    After two workers at the nuclear weapons plant at Oak Ridge National Laboratory in Tennessee were diagnosed earlier this year with chronic beryllium disease (CBD), a rare and sometimes fatal scarring of the lungs, the Department of Energy ordered up a 4-year probe. Now, part of that probe has begun - tests conducted by the Oak Ridge Associated Universities' Center for Epidemiological Research measuring beryllium sensitivity in 3,000 people who've been exposed to the metal's dust since Manhattan Project managers opened the Y-12 plant at Oak Ridge in 1943. Currently, 119 Y-12 employees process beryllium, which has a number of industrial uses, including rocket heat shields and nuclear weapon and electrical components. The disease often takes 20 to 25 years to develop, and the stricken employees haven't worked with beryllium for years. There is no cure for CBD, estimated to strike 2% of people exposed to the metal. Anti-inflammatory steroids alleviate such symptoms as a dry cough, weight loss, and fatigue. Like other lung-fibrosis diseases that are linked to lung cancer, some people suspect CBD might cause some lung cancer. While difficult to diagnose, about 900 cases of CBD have been reported since a Beryllium Case Registry was established in 1952. The Department of Energy (DOE) estimates that about 10,000 DOE employees and 800,000 people in private industry have worked with beryllium.

  14. Abnormally Malicious Autonomous Systems and their Internet Connectivity

    SciTech Connect (OSTI)

    Shue, Craig A; Kalafut, Prof. Andrew; Gupta, Prof. Minaxi

    2011-01-01

    While many attacks are distributed across botnets, investigators and network operators have recently targeted malicious networks through high profile autonomous system (AS) de-peerings and network shut-downs. In this paper, we explore whether some ASes indeed are safe havens for malicious activity. We look for ISPs and ASes that exhibit disproportionately high malicious behavior using ten popular blacklists, plus local spam data, and extensive DNS resolutions based on the contents of the blacklists. We find that some ASes have over 80% of their routable IP address space blacklisted. Yet others account for large fractions of blacklisted IP addresses. Several ASes regularly peer with ASes associated with significant malicious activity. We also find that malicious ASes as a whole differ from benign ones in other properties not obviously related to their malicious activities, such as more frequent connectivity changes with their BGP peers. Overall, we conclude that examining malicious activity at AS granularity can unearth networks with lax security or those that harbor cybercrime.

  15. Form:Reference | Open Energy Information

    Open Energy Info (EERE)

    The reference title should match the book title. e.g.- Where the Wild Things Are Book Review Used when citing a review of a book. The reference title should include "Review of the...

  16. RAPID/Roadmap/12-WA-a | Open Energy Information

    Open Energy Info (EERE)

    take wildlife from the wild without permission from the Washington State Department of Fish and Wildlife (WDFW). The WDFW issues Live Wildlife Taking Permits under WAC 232-12-064....

  17. DOI-BLM-NV-C010-2013-0037-DNA | Open Energy Information

    Open Energy Info (EERE)

    37-DNA Jump to: navigation, search NEPA Document Collection for: DOI-BLM-NV-C010-2013-0037-DNA DNA at Gabbs Valley Geothermal Area for GeothermalWell Field, DNA for Wild Rose Unit...

  18. Science Magazine

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    them into structures sculpted on the nano- meter-scale, and design the macroscopic cell. Some of JCAP's goals are wildly ambi- tious. Researchers plan to characterize a mil-...

  19. Spotlights Archive | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    hosted a Northern Saw-Whet Owl banding demonstration by master bander, Tim Tolford. Bird banding is a technique used to study wild birds by attaching a tag to their leg to...

  20. BPA-2014-01120-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Wild Salmon is a 501(c)(3) tax-exempt non-profit organization of more than 50 sport fishing, commercial fishing, and conservation organizations - local, regional, and...

  1. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    We analyzed the response of soil bacteria to roots of the common annual grass Avena fatua ... associated with a "wild" annual grass, Avena fatua, which is commonly a dominant plant ...

  2. Leveraging Tribal Renewable Resources to Support Military Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    May 30-31, 2013 Image of a speaker at the forum. Chandler, Arizona Wild Horse Pass Hotel and Casino The Office of Indian Energy Tribal Leader Energy Forum on "Leveraging...

  3. BPA-2015-01485-FCorrespondence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FREEDOM OF INFORMATION ACT PROGRAM In reply refer to: FOIA BPA-2015-01485-F Arlene Montgomery Friends of the Wild Swan PO Box 103 Bigfork, MT 59911 Ms. Montgomery: The Department...

  4. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three...

  5. ALSNews Vol. 278

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    from spambots. You need JavaScript enabled to view it To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three...

  6. 2013-02 "Review Material Disposal Areas at LANL in Addition to...

    Broader source: Energy.gov (indexed) [DOE]

    2 is to ensure that the appropriate level of review has been given to MDAs other than MDA G at LANL that may be at similar risk in the event of wild fires. Rec 2013-02 - March 20,...

  7. CX-003608: Categorical Exclusion Determination | Department of...

    Office of Environmental Management (EM)

    in support of two aspects of the use of wild algae for the production of alternative fuels: 1) large scale algal growth and harvest for biofuel production and 2) ...

  8. Best Estimate Radiation Flux Value-Added Procedure: Algorithm...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    H. Hegner, A. Heimo, G. Konig-Langlo, B. McArthur, G. Mller, R. Phillipona, R. Pinker, C. H. Whitlock, K. Dehne, and M. Wild, 1998: Baseline Surface Radiation Network...

  9. ALSNews Vol. 278

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    is being protected from spambots. You need JavaScript enabled to view it To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on...

  10. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three...

  11. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents,...

  12. Hanford Site Black-tailed Jackrabbit

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 Hanford Site Black-tailed Jackrabbit Monitoring Report for Fiscal Year 2013 C. Lindsey, J. Nugent, J. Wilde, and S. Johnson Mission Support Alliance Date Published February 2014...

  13. HNF-56531

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unlimited Hanford Site Burrowing Owl Monitoring Report for Calendar Year 2013 J.W. Wilde, C.T. Lindsey, J.J. Nugent, and M.S. Filan Mission Support Alliance Date...

  14. 180C

    Office of Environmental Management (EM)

    Fragua Council of State Governments-ERC Conrad Smith P. Edwards R. Pinney M. Mulhare C. Richardson M. Vyenielo W. Sherman C. Smith C. Smith E. Wilds C. Smith W. Sherman J. ...

  15. First time nuclear material detection by one short-pulse-laser...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    R. Haight, C. Hamilton, B.M. Hegelich, R.P Johnson, F. Merrill, G. Schaumann, K. Schoenberg, M. Schollmeier, T.Shimada, T. Taddeucci, J. L. Tybo, S. A Wender, C. H Wilde, G. A....

  16. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Further, a C. jejuni growth experiment in minimal medium supplemented with 3-PG showed that this molecule enhances the growth of wild-type C. jejuni, but not of the PEB3 mutants. ...

  17. http://frwebgate.access.gpo.gov/cgi-bin/usc.cgi?ACTION=RETRIEVE

    National Nuclear Security Administration (NNSA)

    Congressional findings and declaration of policy Congress finds and declares that wild free-roaming horses and burros are living symbols of the historic and pioneer spirit of the...

  18. Successional trajectories of rhizosphere bacterial communities...

    Office of Scientific and Technical Information (OSTI)

    We analyzed the response of soil bacteria to roots of the common annual grass Avena fatua ... associated with a "wild" annual grass, Avena fatua, which is commonly a dominant plant ...

  19. Cellobiohydrolase I enzymes

    DOE Patents [OSTI]

    Adney, William S; Himmel, Michael E; Decker, Stephen R; Knoshaug, Eric P; Nimlos, Mark R; Crowley, Michael F; Jeoh, Tina

    2014-01-28

    Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The mutations in the catalytic domain reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. The addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide increases O-linked glycosylation of the isolated polypeptide. Further provided are compositions comprising such polypeptides and nucleic acids encoding such polypeptides. Still further provided are methods for making such polypeptides.

  20. Leveraging Tribal Renewable Resources to Support Military Energy Goals

    Office of Environmental Management (EM)

    LEVERAGING TRIBAL RENEWABLE RESOURCES TO SUPPORT MILITARY ENERGY GOALS May 30-31, 2013 WILD HORSE PASS HOTEL AND CASINO 5040 Wild Horse Pass Blvd. Chandler, Arizona The seventh in a series of planned U.S. DOE Office of Indian Energy-sponsored strategic energy development forums, this Tribal Leader Forum is designed to provide information for western U.S. tribal leaders and military leaders on the renewable energy resource development potential on tribal lands, and the opportunities for

  1. C:\FrontPage Webs\hmas\las_vegas\NWHR2.wpd

    National Nuclear Security Administration (NNSA)

    Location/Habitat The Nevada Wild Horse Range (NWHR) is contained within the northcentral portion of the Nellis Air Force Range (NAFR). The NAFR is located in south-central Nevada in Clark, Lincoln and Nye counties. The NAFR comprises 2,209,326 acres of which approximately 394,000 acres composes the Nevada Wild Horse Range. The Las Vegas District has administrative responsibilities for all land and resource management activities within the NAFR and NWHR. Permanent water sources consist of springs

  2. Mutant HNF-1{alpha} and mutant HNF-1{beta} identified in MODY3 and MODY5 downregulate DPP-IV gene expression in Caco-2 cells

    SciTech Connect (OSTI)

    Gu Ning [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Laboratory of Neurochemistry, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Adachi, Tetsuya [Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto (Japan); Matsunaga, Tetsuro [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Takeda, Jun [Department of Endocrinology Diabetes and Rheumatology, Graduate School of Medicine, Gifu University School of Medicine, Gifu (Japan); Tsujimoto, Gozoh [Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto (Japan); Ishihara, Akihiko [Laboratory of Neurochemistry, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Yasuda, Koichiro [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Diabetic Center, Tsunashimakai-Kosei Hospital, Himeji (Japan); Tsuda, Kinsuke [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan)]. E-mail: jinkan@tom.life.h.kyoto-u.ac.jp

    2006-08-04

    Dipeptidylpeptidase IV (DPP-IV) is a well-documented drug target for the treatment of type 2 diabetes. Hepatocyte nuclear factors (HNF)-1{alpha} and HNF-1{beta}, known as the causal genes of MODY3 and MODY5, respectively, have been reported to be involved in regulation of DPP-IV gene expression. But, it is not completely clear (i) that they play roles in regulation of DPP-IV gene expression, and (ii) whether DPP-IV gene activity is changed by mutant HNF-1{alpha} and mutant HNF-1{beta} in MODY3 and MODY5. To explore these questions, we investigated transactivation effects of wild HNF-1{alpha} and 13 mutant HNF-1{alpha}, as well as wild HNF-1{beta} and 2 mutant HNF-1{beta}, on DPP-IV promoter luciferase gene in Caco-2 cells by means of a transient experiment. Both wild HNF-1{alpha} and wild HNF-1{beta} significantly transactivated DPP-IV promoter, but mutant HNF-1{alpha} and mutant HNF-1{beta} exhibited low transactivation activity. Moreover, to study whether mutant HNF-1{alpha} and mutant HNF-1{beta} change endogenous DPP-IV enzyme activity, we produced four stable cell lines from Caco-2 cells, in which wild HNF-1{alpha} or wild HNF-1{beta}, or else respective dominant-negative mutant HNF-1{alpha}T539fsdelC or dominant-negative mutant HNF-1{beta}R177X, was stably expressed. We found that DPP-IV gene expression and enzyme activity were significantly increased in wild HNF-1{alpha} cells and wild HNF-1{beta} cells, whereas they decreased in HNF-1{alpha}T539fsdelC cells and HNF-1{beta}R177X cells, compared with DPP-IV gene expression and enzyme activity in Caco-2 cells. These results suggest that both wild HNF-1{alpha} and wild HNF-1{beta} have a stimulatory effect on DPP-IV gene expression, but that mutant HNF-1{alpha} and mutant HNF-1{beta} attenuate the stimulatory effect.

  3. THE OHIO RIVER VALLEY CO2 STORAGE PROJECT - PRELIMINARY ASSESSMENT OF DEEP SALINE RESERVOIRS AND COAL SEAMS

    SciTech Connect (OSTI)

    Michael J. Mudd; Howard Johnson; Charles Christopher; T.S. Ramakrishnan, Ph.D.

    2003-08-01

    This report describes the geologic setting for the Deep Saline Reservoirs and Coal Seams in the Ohio River Valley CO{sub 2} Storage Project area. The object of the current project is to site and design a CO{sub 2} injection facility. A location near New Haven, WV, has been selected for the project. To assess geologic storage reservoirs at the site, regional and site-specific geology were reviewed. Geologic reports, deep well logs, hydraulic tests, and geologic maps were reviewed for the area. Only one well within 25 miles of the site penetrates the deeper sedimentary rocks, so there is a large amount of uncertainty regarding the deep geology at the site. New Haven is located along the Ohio River on the border of West Virginia and Ohio. Topography in the area is flat in the river valley but rugged away from the Ohio River floodplain. The Ohio River Valley incises 50-100 ft into bedrock in the area. The area of interest lies within the Appalachian Plateau, on the western edge of the Appalachian Mountain chain. Within the Appalachian Basin, sedimentary rocks are 3,000 to 20,000 ft deep and slope toward the southeast. The rock formations consist of alternating layers of shale, limestone, dolomite, and sandstone overlying dense metamorphic continental shield rocks. The Rome Trough is the major structural feature in the area, and there may be some faults associated with the trough in the Ohio-West Virginia Hinge Zone. The area has a low earthquake hazard with few historical earthquakes. Target injection reservoirs include the basal sandstone/Lower Maryville and the Rose Run Sandstone. The basal sandstone is an informal name for sandstones that overlie metamorphic shield rock. Regional geology indicates that the unit is at a depth of approximately 9,100 ft below the surface at the project site and associated with the Maryville Formation. Overall thickness appears to be 50-100 ft. The Rose Run Sandstone is another potential reservoir. The unit is located approximately 1,100 ft above the basal sandstone and is 100-200 ft thick. The storage capacity estimates for a 20-mile radius from the injection well ranged from 39-78 million tons (Mt) for each formation. Several other oil and gas plays have hydraulic properties conducive for injection, but the formations are generally only 5-50 ft thick in the study area. Overlying the injection reservoirs are thick sequences of dense, impermeable dolomite, limestone, and shale. These layers provide containment above the potential injection reservoirs. In general, it appears that the containment layers are much thicker and extensive than the injection intervals. Other physical parameters for the study area appear to be typical for the region. Anticipated pressures at maximum depths are approximately 4,100 psi based on a 0.45 psi/ft pressure gradient. Temperatures are likely to be 150 F. Groundwater flow is slow and complex in deep formations. Regional flow directions appear to be toward the west-northwest at less than 1 ft per year within the basal sandstone. Vertical gradients are downward in the study area. A review of brine geochemistry indicates that formation fluids have high salinity and dissolved solids. Total dissolved solids ranges from 200,000-325,000 mg/L in the deep reservoirs. Brine chemistry is similar throughout the different formations, suggesting extensive mixing in a mature basin. Unconsolidated sediments in the Ohio River Valley are the primary source of drinking water in the study area.

  4. The Ohio River Valley CO2 Storage Project AEP Mountaineer Plant, West Virginia Numerical Simulation and Risk Assessment Report

    SciTech Connect (OSTI)

    Neeraj Gupta

    2008-03-31

    A series of numerical simulations of carbon dioxide (CO{sub 2}) injection were conducted as part of a program to assess the potential for geologic sequestration in deep geologic reservoirs (the Rose Run and Copper Ridge formations), at the American Electric Power (AEP) Mountaineer Power Plant outside of New Haven, West Virginia. The simulations were executed using the H{sub 2}O-CO{sub 2}-NaCl operational mode of the Subsurface Transport Over Multiple Phases (STOMP) simulator (White and Oostrom, 2006). The objective of the Rose Run formation modeling was to predict CO{sub 2} injection rates using data from the core analysis conducted on the samples. A systematic screening procedure was applied to the Ohio River Valley CO{sub 2} storage site utilizing the Features, Elements, and Processes (FEP) database for geological storage of CO{sub 2} (Savage et al., 2004). The objective of the screening was to identify potential risk categories for the long-term geological storage of CO{sub 2} at the Mountaineer Power Plant in New Haven, West Virginia. Over 130 FEPs in seven main classes were assessed for the project based on site characterization information gathered in a geological background study, testing in a deep well drilled on the site, and general site conditions. In evaluating the database, it was apparent that many of the items were not applicable to the Mountaineer site based its geologic framework and environmental setting. Nine FEPs were identified for further consideration for the site. These FEPs generally fell into categories related to variations in subsurface geology, well completion materials, and the behavior of CO{sub 2} in the subsurface. Results from the screening were used to provide guidance on injection system design, developing a monitoring program, performing reservoir simulations, and other risk assessment efforts. Initial work indicates that the significant FEPs may be accounted for by focusing the storage program on these potential issues. The screening method was also useful in identifying unnecessary items that were not significant given the site-specific geology and proposed scale of the Ohio River Valley CO{sub 2} Storage Project. Overall, the FEP database approach provides a comprehensive methodology for assessing potential risk for a practical CO{sub 2} storage application. An integrated numerical fate and transport model was developed to enable risk and consequence assessment at field scale. Results show that such an integrated modeling effort would be helpful in meeting the project objectives (such as site characterization, engineering, permitting, monitoring and closure) during different stages. A reservoir-scale numerical model was extended further to develop an integrated assessment framework which can address the risk and consequence assessment, monitoring network design and permitting guidance needs. The method was used to simulate sequestration of CO{sub 2} in moderate quantities at the Mountaineer Power Plant. Results indicate that at the relatively low injection volumes planned for pilot scale demonstration at this site, the risks involved are minor to negligible, owing to a thick, low permeability caprock and overburden zones. Such integrated modeling approaches coupled with risk and consequence assessment modeling are valuable to project implementation, permitting, monitoring as well as site closure.

  5. LNG Safety Research: FEM3A Model Development

    SciTech Connect (OSTI)

    Iraj A. Salehi; Jerry Havens; Tom Spicer

    2006-09-30

    The initial scope of work for this project included: (1) Improving the FEM3A advanced turbulence closure module, (2) Adaptation of FEM3A for more general applications, and (3) Verification of dispersion over rough surfaces, with and without obstacle using the advanced turbulence closure module. These work elements were to be performed by Chemical Hazards Research Center (CHRC), Department of Chemical Engineering, University of Arkansas as a subcontractor to Gas Technology Institute (GTI). The tasks for GTI included establishment of the scientific support base for standardization of the FEM3A model, project management, technology transfer, and project administration. Later in the course of the project, the scope of work was modified by the National Energy Technology Laboratories (NETL) to remove the emphasis on FEM3A model and instead, develop data in support of NETL's FLUENT modeling. With this change, GTI was also instructed to cease activities relative to FEM3A model. GTI's technical activities through this project included the initial verification of FEM3A model, provision of technical inputs to CHRC researchers regarding the structure of the final product, and participation in technical discussion sessions with CHRC and NETL technical staff. GTI also began the development of a Windows-based front end for the model but the work was stopped due to the change in scope of work. In the meantime, GTI organized a workshop on LNG safety in Houston, Texas. The workshop was very successful and 75 people from various industries participated. All technical objectives were met satisfactorily by Dr. Jerry Havens and Dr. Tom Spicer of CHRC and results are presented in a stand-alone report included as Appendix A to this report.

  6. LNG Safety Research: FEM3A Model Development

    SciTech Connect (OSTI)

    2006-09-30

    The initial scope of work for this project included: 1) Improving the FEM3A advanced turbulence closure module, 2) Adaptation of FEM3A for more general applications, and 3) Verification of dispersion over rough surfaces, with and without obstacle using the advanced turbulence closure module. These work elements were to be performed by Chemical Hazards Research Center (CHRC), Department of Chemical Engineering, University of Arkansas as a subcontractor to Gas Technology Institute (GTI). The tasks for GTI included establishment of the scientific support base for standardization of the FEM3A model, project management, technology transfer, and project administration. Later in the course of the project, the scope of work was modified by the National Energy Technology Laboratories (NETL) to remove the emphasis on FEM3A model and instead, develop data in support of NETL’s FLUENT modeling. With this change, GTI was also instructed to cease activities relative to FEM3A model. GTI’s technical activities through this project included the initial verification of FEM3A model, provision of technical inputs to CHRC researchers regarding the structure of the final product, and participation in technical discussion sessions with CHRC and NETL technical staff. GTI also began the development of a Windows-based front end for the model but the work was stopped due to the change in scope of work. In the meantime, GTI organized a workshop on LNG safety in Houston, Texas. The workshop was very successful and 75 people from various industries participated. All technical objectives were met satisfactorily by Dr. Jerry Havens and Dr. Tom Spicer of CHRC and results are presented in a stand-alone report included as Appendix A to this report.

  7. WILDFIRE IGNITION RESISTANCE ESTIMATOR WIZARD SOFTWARE DEVELOPMENT REPORT

    SciTech Connect (OSTI)

    Phillips, M.; Robinson, C.; Gupta, N.; Werth, D.

    2012-10-10

    This report describes the development of a software tool, entitled WildFire Ignition Resistance Estimator Wizard (WildFIRE Wizard, Version 2.10). This software was developed within the Wildfire Ignition Resistant Home Design (WIRHD) program, sponsored by the U. S. Department of Homeland Security, Science and Technology Directorate, Infrastructure Protection & Disaster Management Division. WildFIRE Wizard is a tool that enables homeowners to take preventive actions that will reduce their homes vulnerability to wildfire ignition sources (i.e., embers, radiant heat, and direct flame impingement) well in advance of a wildfire event. This report describes the development of the software, its operation, its technical basis and calculations, and steps taken to verify its performance.

  8. Divinyl ether synthase gene, and protein and uses thereof

    DOE Patents [OSTI]

    Howe, Gregg A.; Itoh, Aya

    2006-12-26

    The present invention relates to divinyl ether synthase genes, proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also relates to methods of using divinyl ether synthase genes and proteins, including in their expression in transgenic organisms and in the production of divinyl ether fatty acids, and to methods of suing divinyl ether fatty acids, including in the protection of plants from pathogens.

  9. Divinyl ether synthase gene and protein, and uses thereof

    DOE Patents [OSTI]

    Howe, Gregg A. (East Lansing, MI); Itoh, Aya (Tsuruoka, JP)

    2011-09-13

    The present invention relates to divinyl ether synthase genes, proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also relates to methods of using divinyl ether synthase genes and proteins, including in their expression in transgenic organisms and in the production of divinyl ether fatty acids, and to methods of suing divinyl ether fatty acids, including in the protection of plants from pathogens.

  10. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public

  11. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public

  12. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public

  13. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Structure and Receptor Specificity of an Avian Flu Antigen Print Wednesday, 25 July 2007 00:00 To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of

  14. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public health

  15. CONSEQUENCES OF ALTERING RUBISCO REGULATION

    SciTech Connect (OSTI)

    Salvucci, Michael

    2013-12-31

    Research examined the thermal stability and propensity for aggregation of wild type and the C- and N-terminally modified forms of activase to determine if loss of activity under heat stress is dependent on protein aggregation. The results showed that 1) loss of activity at high temperature is independent of aggregation; 2) activase with both C- and N-terminal S-Tags are more susceptible to aggregation than wild type activase, 3) aggregation is highly dependent on the concentration of Mg2+ and 4) the ATP analog, ATPgammaS, protects against both thermal inactivation and aggregation.

  16. Regioselective alkane hydroxylation with a mutant AlkB enzyme

    DOE Patents [OSTI]

    Koch, Daniel J.; Arnold, Frances H.

    2012-11-13

    AlkB from Pseudomonas putida was engineered using in-vivo directed evolution to hydroxylate small chain alkanes. Mutant AlkB-BMO1 hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. Mutant AlkB-BMO2 similarly hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. These biocatalysts are highly active for small chain alkane substrates and their regioselectivity is retained in whole-cell biotransformations.

  17. EA-1895: Lolo Creek Permanent Weir Construction near town of Weippe, Clearwater County, Idaho

    Broader source: Energy.gov [DOE]

    DOEs Bonneville Power Administration was preparing this EA to evaluate the potential environmental impacts of replacing an existing seasonal fish weir with a permanent weir, which would have been used to monitor federally-listed Snake River steelhead and collect spring Chinook salmon adults to support ongoing supplementation programs in the watershed. The Bureau of Land Management, a cooperating agency, preliminarily determined Lolo Creek to be suitable for Congressional designation into the Wild and Scenic River System. The EA included a Wild and Scenic River Section 7 analysis. This project was canceled.

  18. NAFOA 34th Annual Conference | Department of Energy

    Energy Savers [EERE]

    NAFOA 34th Annual Conference NAFOA 34th Annual Conference April 17, 2016 8:00AM MST to April 19, 2016 5:00PM MST Chandler, Arizona Sheraton Wild Horse Pass Resort & Spa 5594 W. Wild Horse Pass Rd. Chandler, AZ 85226 The Native American Finance Officers Association (NAFOA) is hosting its annual conference to give an opportunity to not only learn more about economic and financial issues facing Indian Country, but it will also provide attendees a chance to engage with tribal leaders,

  19. Using weeds to fight wastes

    SciTech Connect (OSTI)

    1992-10-01

    Researchers at Los Alamos National Laboratory and New Mexico State University have discovered that jimson weed and wild tomato plants can remove the toxic wastes in wastewater associated with the production of trinitrotoluene (TNT). According to Wolfgang F. Mueller of New Mexico State, tissue-cultured cells of jimson weed rapidly absorb and break down toxic and carcinogenic elements in {open_quotes}pink water,{close_quotes} a by-product of the manufacture of TNT. Mueller and his colleagues have found similar results with the wild tomato plant.

  20. Structure of adenovirus bound to cellular receptor car

    DOE Patents [OSTI]

    Freimuth, Paul I.

    2007-01-02

    Disclosed is a mutant CAR-DI-binding adenovirus which has a genome comprising one or more mutations in sequences which encode the fiber protein knob domain wherein the mutation causes the encoded viral particle to have a significantly weakened binding affinity for CAR-DI relative to wild-type adenovirus. Such mutations may be in sequences which encode either the AB loop, or the HI loop of the fiber protein knob domain. Specific residues and mutations are described. Also disclosed is a method for generating a mutant adenovirus which is characterized by a receptor binding affinity or specificity which differs substantially from wild type.

  1. Keep in mind, that with formality, often comes more of a standing presence of a committee within DOE-it gets into DOE's system and becomes a budget line item as well

    Office of Environmental Management (EM)

    ICEIWG May 29, 2013 WILD HORSE PASS HOTEL & CASINO 5040 Wild Horse Pass Blvd., Chandler, AZ AGENDA CONFERENCE ROOM: ACACIA CD WEDNESDAY, MAY 29, 2013 8:00-9:00am REGISTRATION & BREAKFAST ** Continental breakfast will be provided on-site. ** 9:00-11:00am WELCOME & INTRODUCTIONS Opening Prayer by ICEIWG Tribal Representative Tracey A. LeBeau, Director, U.S. DOE Office of Indian Energy (IE) & DOE ICEIWG Co-Chair Jim Manion, Warm Springs Delegate & Tribal ICEIWG Co-Chair Pilar

  2. Indian Country Energy and Infrastructure Working Group (ICEIWG) January 26, 2012

    Office of Environmental Management (EM)

    ICEIWG January 26, 2012 GILA RIVER INDIAN COMMUNITY Wild Horse Pass Hotel & Casino 5040 Wild Horse Pass Boulevard Chandler, AZ 85226 (520) 796-7272 Action Items DRAFT PURCHASING POLICY GUIDANCE  The draft policy guidance was distributed to ICEIWG at the Portland meeting in October 2011. IE is accepting comments from ICEIWG as they arise.  The goal is to get final comments and to work through the internal review process. Once that is finalized, it will go through the formal leadership

  3. 2013-02 "Review Material Disposal Areas at LANL in Addition to Technical Area 54 for Risks Associated with Fires"

    Broader source: Energy.gov [DOE]

    Approved March 20, 2013 The intent of this Recommendation 2013‐02 is to ensure that the appropriate level of review has been given to MDAs other than MDA G at LANL that may be at similar risk in the event of wild fires.

  4. Stock Assessment of Columbia River Anadromous Salmonids : Final Report, Volume I, Chinook, Coho, Chum and Sockeye Salmon Summaries.

    SciTech Connect (OSTI)

    Howell, Philip J.

    1986-07-01

    The purpose was to identify and characterize the wild and hatchery stocks of salmon and steelhead in the Columbia River Basin on the basis of currently available information. This report provides a comprehensive compilation of data on the status and life histories of Columbia Basin salmonid stocks.

  5. 1998 - 07 | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    July 1998 Thu, 07/02/1998 - 12:00am Scientists Taking Laser on Wild Ride; Physicists Stretch Beam's Capabilities (Richmond Times Dispatch) Wed, 07/01/1998 - 12:00am Researchers Break Power Record for FEL (Opto Electronic News

  6. Idaho Habitat/Natural Production Monitoring Part I, 1995 Annual Report.

    SciTech Connect (OSTI)

    Hall-Griswold, J.A.; Petrosky, C.E.

    1996-12-01

    The Idaho Department of Fish and Game (IDFG) has been monitoring trends in juvenile spring and summer chinook salmon, Oncorhynchus tshawytscha, and steelhead trout, O. mykiss, populations in the Salmon, Clearwater, and lower Snake River drainages for the past 12 years. This work is the result of a program to protect, mitigate, and enhance fish and wildlife affected by the development and operation of hydroelectric power plants on the Columbia River. Project 91-73, Idaho Natural Production Monitoring, consists of two subprojects: General Monitoring and Intensive Monitoring. This report updates and summarizes data through 1995 for the General Parr Monitoring (GPM) database to document status and trends of classes of wild and natural chinook salmon and steelhead trout populations. A total of 281 stream sections were sampled in 1995 to monitor trends in spring and summer chinook salmon Oncorhynchus tshawytscha and steelhead trout O. mykiss parr populations in Idaho. Percent carrying capacity and density estimates were summarized for 1985--1995 by different classes of fish: wild A-run steelhead trout, wild B-run steelhead trout, natural A-run steelhead trout, natural B-run steelhead trout, wild spring and summer chinook salmon, and natural spring and summer chinook salmon. The 1995 data were also summarized by subbasins as defined in Idaho Department of Fish and Game`s 1992--1996 Anadromous Fish Management Plan.

  7. Technical Basis Agreement Document for UGTA CAU 99 RM/SM

    National Nuclear Security Administration (NNSA)

    D.L. Finnegan, J.L. Thompson, C.M. Miller, P.L. Baca, L.F. Olivas, C.G. Geoffrion, D.K. Smith, W. Goishi, B.K. Esser, J.W. Meadows, N. Namboodiri, J.F. Wild. 2001. Nevada Test Site...

  8. Lawrence Co. Scioto Co. Greenup Co. Jack

    U.S. Energy Information Administration (EIA) Indexed Site

    CROOK PYR AMI D AU XIER LEF T F OR K B CUCU MBER CRK CHANEY CREEK DINGUS RPD-SC IOT O-3 MOORE BRANC H RPD-TAZ EWELL-1 PORT ER CAMP MOU SIE WILD CAT HOLLOW SPR ING CREEK RACCOON ...

  9. Deficiency in Homologous Recombination Renders Mammalian Cells More Sensitive to Proton Versus Photon Irradiation

    SciTech Connect (OSTI)

    Grosse, Nicole; Fontana, Andrea O. [Laboratory for Molecular Radiobiology, University Hospital Zurich, Zurich (Switzerland); Hug, Eugen B.; Lomax, Antony; Coray, Adolf [Center for Proton Therapy, Paul Scherrer Institute, Villigen (Switzerland); Augsburger, Marc [Laboratory for Molecular Radiobiology, University Hospital Zurich, Zurich (Switzerland); Paganetti, Harald [Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts (United States); Sartori, Alessandro A. [Institute of Molecular Cancer Research, University of Zurich, Zurich (Switzerland); Pruschy, Martin, E-mail: martin.pruschy@usz.ch [Laboratory for Molecular Radiobiology, University Hospital Zurich, Zurich (Switzerland)

    2014-01-01

    Purpose: To investigate the impact of the 2 major DNA repair machineries on cellular survival in response to irradiation with the 2 types of ionizing radiation. Methods and Materials: The DNA repair and cell survival endpoints in wild-type, homologous recombination (HR)-deficient, and nonhomologous end-joining-deficient cells were analyzed after irradiation with clinically relevant, low-linear energy transfer (LET) protons and 200-keV photons. Results: All cell lines were more sensitive to proton irradiation compared with photon irradiation, despite no differences in the induction of DNA breaks. Interestingly, HR-deficient cells and wild-type cells with small interfering RNA-down-regulated Rad51 were markedly hypersensitive to proton irradiation, resulting in an increased relative biological effectiveness in comparison with the relative biological effectiveness determined in wild-type cells. In contrast, lack of nonhomologous end-joining did not result in hypersensitivity toward proton irradiation. Repair kinetics of DNA damage in wild-type cells were equal after both types of irradiation, although proton irradiation resulted in more lethal chromosomal aberrations. Finally, repair kinetics in HR-deficient cells were significantly delayed after proton irradiation, with elevated amounts of residual ?H2AX foci after irradiation. Conclusion: Our data indicate a differential quality of DNA damage by proton versus photon irradiation, with a specific requirement for homologous recombination for DNA repair and enhanced cell survival. This has potential relevance for clinical stratification of patients carrying mutations in the DNA damage response pathways.

  10. Survival Estimates for the Passage of Spring-Migrating Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2008.

    SciTech Connect (OSTI)

    Faulkner, James R.; Smith, Steven G.; Muir, William D.

    2009-06-23

    In 2008, the National Marine Fisheries Service completed the sixteenth year of a study to estimate survival and travel time of juvenile salmonids Oncorhynchus spp. passing through dams and reservoirs on the Snake and Columbia Rivers. All estimates were derived from detections of fish tagged with passive integrated transponder (PIT) tags. We PIT tagged and released a total of 18,565 hatchery steelhead O. mykiss, 15,991 wild steelhead, and 9,714 wild yearling Chinook salmon O. tshawytscha at Lower Granite Dam in the Snake River. In addition, we utilized fish PIT tagged by other agencies at traps and hatcheries upstream from the hydropower system and at sites within the hydropower system in both the Snake and Columbia Rivers. These included 122,061 yearling Chinook salmon tagged at Lower Granite Dam for evaluation of latent mortality related to passage through Snake River dams. PIT-tagged smolts were detected at interrogation facilities at Lower Granite, Little Goose, Lower Monumental, Ice Harbor, McNary, John Day, and Bonneville Dams and in the PIT-tag detector trawl operated in the Columbia River estuary. Survival estimates were calculated using a statistical model for tag-recapture data from single release groups (the single-release model). Primary research objectives in 2008 were to: (1) estimate reach survival and travel time in the Snake and Columbia Rivers throughout the migration period of yearling Chinook salmon and steelhead, (2) evaluate relationships between survival estimates and migration conditions, and (3) evaluate the survival estimation models under prevailing conditions. This report provides reach survival and travel time estimates for 2008 for PIT-tagged yearling Chinook salmon (hatchery and wild), hatchery sockeye salmon O. nerka, hatchery coho salmon O. kisutch, and steelhead (hatchery and wild) in the Snake and Columbia Rivers. Additional details on the methodology and statistical models used are provided in previous reports cited here. Survival and detection probabilities were estimated precisely for most of the 2008 yearling Chinook salmon and steelhead migrations. Hatchery and wild fish were combined in some of the analyses. For yearling Chinook salmon, overall percentages for combined release groups used in survival analyses in the Snake River were 80% hatchery-reared and 20% wild. For steelhead, the overall percentages were 65% hatchery-reared and 35% wild. Estimated survival from the tailrace of Lower Granite Dam to the tailrace of Little Goose Dam averaged 0.939 for yearling Chinook salmon and 0.935 for steelhead.

  11. CARBON SEQUESTRATION IN RECLAIMED MINED SOILS OF OHIO

    SciTech Connect (OSTI)

    M.K. Shukla; R. Lal

    2004-10-01

    This research project is aimed at assessing the soil organic carbon (SOC) sequestration potential of reclaimed minesoils (RMS). The experimental sites, owned and maintained by the American Electrical Power, are located in Guernsey, Morgan, Noble, and Muskingum Counties of Ohio. These sites, characterized by age chronosequences, were reclaimed with and without topsoil application and are under continuous grass or forest cover. During this quarter, water infiltration tests were performed on the soil surface in the experimental sites. Soil samples were analyzed for the soil carbon and nitrogen contents, texture, water stable aggregation, and mean weight and geometric mean diameter of aggregates. This report presents the results from two sites reclaimed during 1978 and managed under grass (Wilds) and forest (Cumberland) cover, respectively. The trees were planted in 1982 in the Cumberland site. The analyses of data on soil bulk density ({rho}{sub b}), SOC and total nitrogen (TN) concentrations and stocks were presented in the third quarter report. This report presents the data on infiltration rates, volume of transport and storage pores, available water capacity (AWC) of soil, particle size distribution, and soil inorganic carbon (SIC) and coal carbon contents. The SIC content ranged from 0.04 to 1.68% in Cumberland tree site and 0.01 to 0.65% in the Wilds. The coal content assumed to be the carbon content after oven drying the sample at 350 C varied between 0.04 and 3.18% for Cumberland and 0.06 and 3.49% for Wilds. The sand, silt and clay contents showed moderate to low variability (CV < 0.16) for 0-15 and 15-30 cm depths. The volume of transmission (VTP) and storage pores (VSP) also showed moderate to high variability (CV ranged from 0.22 to 0.39 for Wilds and 0.17 to 0.36 for Cumberland). The CV for SIC was high (0.7) in Cumberland whereas that for coal content was high (0.4) in the Wilds. The steady state infiltration rates (i{sub c}) also showed high variability (CV > 0.6) and ranged from 0.01 to 0.98 cm min{sup -1} in Cumberland and 0.1 to 1.68 cm min{sup -1} in Wilds. The cumulative infiltration (I) was highly variable (CV > 0.6) and ranged from 4.2 to 110 cm in Cumberland and 17.4 to 250 cm in Wilds. The AWC for 0-15 cm depth also showed moderate variability (CV = 0.3) for Cumberland but high for Wilds (CV = 0.4). The sand and silt contents showed strong spatial dependence with nugget-sill ratio of 15 and 23%, respectively with a range of 50 m in Cumberland site. Strong spatial dependence for sand content was also obtained for Wilds. The VSP, AWC, I, clay content, VTP, and i{sub c}, showed moderate to low spatial dependence (nugget-sill ratio varied from 32 to 72% in Cumberland and 37 to 88% in Wilds). These preliminary results along with those reported earlier during the third quarter suggest that the management effects are important and indicative of these sources of variability.

  12. The role of intrahepatic CD3 +/CD4 −/CD8 − double negative T (DN T) cells in enhanced acetaminophen toxicity

    SciTech Connect (OSTI)

    Getachew, Yonas; Cusimano, Frank A.; James, Laura P.; Thiele, Dwain L.

    2014-10-15

    The role of the immune system, specifically NK, NKT and CD3 cells, in acetaminophen (APAP) induced liver injury remains inconsistently defined. In the present study, wild type (C57BL/6J) mice and granzyme B deficient (GrB −/−) mice were treated with acetaminophen to assess the role of the immune system in acute liver injury. Doses of acetaminophen that induced sub lethal liver injury in wild type mice unexpectedly produced fatal hepatotoxicity in granzyme B deficient (GrB −/−) mice. Analysis revealed that GrB −/− mice had an increased population of intrahepatic CD3 (+), CD4 (−), and CD8 (−) lymphocytes expressing the CD69 activation marker and Fas ligand. Depletion of these cells in the GrB −/− and wild type mice made them less susceptible to APAP injury, while depletion of NK1.1 (+) cells or both CD4 (+) and CD8 (+) T cells failed to provide the same hepatoprotection. Transfer of the GrB −/− IHLs further exacerbated liver injury and increased mortality in wild type mice but not in LRP/LPR mice, lacking fas expression. Conclusions: Acetaminophen toxicity is enhanced by the presence of activated, FasL expressing intrahepatic CD3 (+), CD4 (−), CD8 (−), NK1.1 (−) T cells. Depletion of these cells from GrB −/− mice and wild type mice greatly reduces mortality and improves the course of liver injury recovery. - Highlights: • Intrahepatic lymphocytes (IHLs) from GrB −/− mice harbor activated DNT cells. • IHLs from GrB −/− mice exhibit enhanced Fas ligand expression. • Acetaminophen toxicity is enhanced by activated, FasL expressing DNT cells.

  13. Evidence that an internal carbonic anhydrase is present in 5% CO/sub 2/-grown and air-grown Chlamydomonas. [Chlamydomonas reinhardtii

    SciTech Connect (OSTI)

    Moroney, J.V.; Togasaki, R.K.; Husic, H.D.; Tolbert, N.E.

    1987-07-01

    Inorganic carbon (C/sub i/) uptake was measured in wild-type cells of Chlamydomonas reinhardtii, and in cia-3, a mutant strain of C. reinhardtii that cannot grow with air levels of CO/sub 2/. Both air-grown cells, that have a CO/sub 2/ concentrating system, and 5% CO/sub 2/-grown cells that do not have this system, were used. When the external pH was 5.1 or 7.3, air-grown, wild-type cells accumulated inorganic carbon (C/sub i/) and this accumulation was enhanced when the permeant carbonic anhydrase inhibitor, ethoxyzolamide, was added. When the external pH was 5.1, 5% CO/sub 2/-grown cells also accumulated some C/sub i/, although not as much as air-grown cells and this accumulation was stimulated by the addition of ethoxyzolamide. At the same time, ethoxyzolamide inhibited CO/sub 2/ fixation by high CO/sub 2/-grown, wild-type cells at both pH 5.1 and 7.3. These observations imply that 5% CO/sub 2/-grown, wild-type cells, have a physiologically important internal carbonic anhydrase, although the major carbonic anhydrase located in the periplasmic space is only present in air-grown cells. Inorganic carbon uptake by cia-3 cells supported this conclusion. This mutant strain, which is thought to lack an internal carbonic anhydrase, was unaffected by ethoxyzolamide at pH 5.1. Other physiological characteristics of cia-3 resemble those of wild-type cells that have been treated with ethoxyzolamide. It is concluded that an internal carbonic anhydrase is under different regulatory control than the periplasmic carbonic anhydrase.

  14. Advanced tangentially fired low-NO{sub x} combustion demonstration. Phase 2, LNCFS Level 2 tests

    SciTech Connect (OSTI)

    Smith, L.L.; Hooper, M.

    1993-08-01

    This report summarizes the activities and results for the second testing phase (Phase 2) of an Innovative Clean Coal Technology (ICCT) demonstration of advanced tangentially fired combustion techniques for the reduction of nitrogen oxide (NOx) emissions from coal-fired boilers. All three levels of Asea Brown Boveri Combustion Engineering Service`s (ABB CE`s) Low-NO{sub x} Concentric Firing System (LNCFS) are being demonstrated during this project. The primary goal of this project is to demonstrate the NO{sub x} emissions characteristics of these technologies when operated under normal load dispatched conditions. The equipment is being tested at Gulf Power Company`s Plant Lansing Smith Unit 2 in Lynn Haven, Florida. The long-term NO{sub x} emission trends were documented while the unit was operating under normal load dispatch conditions with the LNCFS Level II equipment. Fifty-five days of long-term data were collected. The data included the effects of mill patterns, unit load, mill outages, weather, fuel variability, and load swings. Test results indicated full-load (180 MW) NO{sub x} emissions of 0.39 lb/MBtu, which is about equal to the short-term test results. At 110 MW, long-term NO{sub x} emissions increased to 0.42 lb/MBtu, which are slightly higher than the short-term data. At 75 MW, NO{sub x} emissions were 0.51 lb/MBtu, which is significantly higher than the short-term data. The annual and 30-day average achievable NOx emissions were determined to be 0.41 and 0.45 lb/MBtu, respectively, for long-term testing load scenarios. NO{sub x} emissions were reduced by a maximum of 40 percent when compared to the baseline data collected in the previous phase. The long-term NO{sub x} reduction at full load (180 MW) was 37 percent while NO{sub x} reduction at low load was minimal.

  15. Intrusion detection and monitoring for wireless networks.

    SciTech Connect (OSTI)

    Thomas, Eric D.; Van Randwyk, Jamie A.; Lee, Erik J.; Stephano, Amanda; Tabriz, Parisa; Pelon, Kristen; McCoy, Damon (University of Colorado, Boulder); Lodato, Mark; Hemingway, Franklin; Custer, Ryan P.; Averin, Dimitry; Franklin, Jason; Kilman, Dominique Marie

    2005-11-01

    Wireless computer networks are increasing exponentially around the world. They are being implemented in both the unlicensed radio frequency (RF) spectrum (IEEE 802.11a/b/g) and the licensed spectrum (e.g., Firetide [1] and Motorola Canopy [2]). Wireless networks operating in the unlicensed spectrum are by far the most popular wireless computer networks in existence. The open (i.e., proprietary) nature of the IEEE 802.11 protocols and the availability of ''free'' RF spectrum have encouraged many producers of enterprise and common off-the-shelf (COTS) computer networking equipment to jump into the wireless arena. Competition between these companies has driven down the price of 802.11 wireless networking equipment and has improved user experiences with such equipment. The end result has been an increased adoption of the equipment by businesses and consumers, the establishment of the Wi-Fi Alliance [3], and widespread use of the Alliance's ''Wi-Fi'' moniker to describe these networks. Consumers use 802.11 equipment at home to reduce the burden of running wires in existing construction, facilitate the sharing of broadband Internet services with roommates or neighbors, and increase their range of ''connectedness''. Private businesses and government entities (at all levels) are deploying wireless networks to reduce wiring costs, increase employee mobility, enable non-employees to access the Internet, and create an added revenue stream to their existing business models (coffee houses, airports, hotels, etc.). Municipalities (Philadelphia; San Francisco; Grand Haven, MI) are deploying wireless networks so they can bring broadband Internet access to places lacking such access; offer limited-speed broadband access to impoverished communities; offer broadband in places, such as marinas and state parks, that are passed over by traditional broadband providers; and provide themselves with higher quality, more complete network coverage for use by emergency responders and other municipal agencies. In short, these Wi-Fi networks are being deployed everywhere. Much thought has been and is being put into evaluating cost-benefit analyses of wired vs. wireless networks and issues such as how to effectively cover an office building or municipality, how to efficiently manage a large network of wireless access points (APs), and how to save money by replacing an Internet service provider (ISP) with 802.11 technology. In comparison, very little thought and money are being focused on wireless security and monitoring for security purposes.

  16. Smolt Monitoring at the Head of Lower Granite Reservoir and Lower Granite Dam, 2005 Annual Report.

    SciTech Connect (OSTI)

    Buettner, Edwin W.; Putnam, Scott A.

    2009-02-18

    This project monitored the daily passage of Chinook salmon Oncorhynchus tshawytscha, steelhead trout O. mykiss, and sockeye salmon O. nerka smolts during the 2005 spring out-migration at migrant traps on the Snake River and Salmon River. In 2005 fish management agencies released significant numbers of hatchery Chinook salmon and steelhead trout above Lower Granite Dam that were not marked with a fin clip or coded-wire tag. Generally, the age-1 and older fish were distinguishable from wild fish by the occurrence of fin erosion. Age-0 Chinook salmon are more difficult to distinguish between wild and non-adclipped hatchery fish and therefore classified as unknown rearing. The total annual hatchery spring/summer Chinook salmon catch at the Snake River trap was 0.34 times greater in 2005 than in 2004. The wild spring/summer Chinook catch was 0.34 times less than the previous year. Hatchery steelhead trout catch was 0.67 times less than in 2004. Wild steelhead trout catch was 0.72 times less than the previous year. The Snake River trap collected 1,152 age-0 Chinook salmon of unknown rearing. During 2005, the Snake River trap captured 219 hatchery and 44 wild/natural sockeye salmon and 110 coho salmon O. kisutch of unknown rearing. Differences in trap catch between years are due to fluctuations not only in smolt production, but also differences in trap efficiency and duration of trap operation associated with flow. Trap operations began on March 6 and were terminated on June 3. The trap was out of operation for a total of one day due to heavy debris. FPC requested that the trap be restarted on June 15 through June 22 to collect and PIT tag age-0 Chinook salmon. Hatchery Chinook salmon catch at the Salmon River trap was 1.06 times greater and wild Chinook salmon catch was 1.26 times greater than in 2004. The hatchery steelhead trout collection in 2005 was 1.41 times greater and wild steelhead trout collection was 1.27 times greater than the previous year. Trap operations began on March 6 and were terminated on May 17 due to high flows. There were two days when the trap was taken out of service because of mechanical failure. Travel time (d) and migration rate (km/d) through Lower Granite Reservoir for passive integrated transponder (PIT) tagged Chinook salmon and steelhead trout marked at the Snake River trap were affected by discharge. Statistical analysis of 2005 data detected a relation between migration rate and discharge for hatchery Chinook but was unable to detect a relation for wild Chinook. The inability to detect a migration rate discharge relation for wild Chinook salmon was caused by a lack of data. For hatchery Chinook salmon there was a 1.8-fold increase in migration rate between 50 and 100 kcfs. For steelhead trout tagged at the Snake River trap, statistical analysis detected a significant relation between migration rate and lower Granite Reservoir inflow discharge. For hatchery and wild steelhead trout, there was a 2.2-fold and a 2.2-fold increase in migration rate, respectively, between 50 and 100 kcfs. Travel time and migration rate to Lower Granite Dam for fish marked at the Salmon River trap were calculated. Statistical analysis of the 2005 data detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge for hatchery Chinook salmon, wild Chinook salmon, hatchery steelhead trout, and wild steelhead trout. Migration rate increased 4.2-fold for hatchery Chinook salmon, 2.9-fold for wild Chinook salmon and 2.5-fold for hatchery steelhead, and 1.7-fold for wild steelhead as discharge increased between 50 kcfs and 100 kcfs. Fish tagged with PIT tags at the Snake River and Salmon River traps were interrogated at four dams with PIT tag detection systems (Lower Granite, Little Goose, Lower Monumental and McNary dams). Because of the addition of the fourth interrogation site (Lower Monumental) in 1993 and the installation of the Removable Spillway Weir at Lower Granite Dam in 2001, caution must be used in comparing cumulative interrogation data. Cumulative interrogations at the fo

  17. Smolt Monitoring at the Head of Lower Granite Reservoir and Lower Granite Dam, 2002 Annual Report.

    SciTech Connect (OSTI)

    Buettner, Edwin W.; Putnam, Scott A.

    2009-02-18

    This project monitored the daily passage of Chinook salmon Oncorhynchus tshawytscha, steelhead trout O. mykiss, and sockeye salmon smolts O. nerka during the 2002 spring out-migration at migrant traps on the Snake River and Salmon River. In 2002 fish management agencies released significant numbers of hatchery Chinook salmon and steelhead trout above Lower Granite Dam that were not marked with a fin clip or coded-wire tag. Generally, these fish were distinguishable from wild fish by the occurrence of fin erosion. Total annual hatchery Chinook salmon catch at the Snake River trap was 11.4 times greater in 2002 than in 2001. The wild Chinook catch was 15.5 times greater than the previous year. Hatchery steelhead trout catch was 2.9 times greater than in 2001. Wild steelhead trout catch was 2.8 times greater than the previous year. The Snake River trap collected 3,996 age-0 Chinook salmon of unknown rearing. During 2002, the Snake River trap captured 69 hatchery and 235 wild/natural sockeye salmon and 114 hatchery coho salmon O. kisutch. Differences in trap catch between years are due to fluctuations not only in smolt production, but also differences in trap efficiency and duration of trap operation associated with flow. The significant increase in catch in 2002 was due to a 3.1 fold increase in hatchery Chinook production and a more normal spring runoff. Trap operations began on March 10 and were terminated on June 7. The trap was out of operation for a total of four days due to mechanical failure or debris. Hatchery Chinook salmon catch at the Salmon River trap was 4.2 times greater and wild Chinook salmon catch was 2.4 times greater than in 2001. The hatchery steelhead trout collection in 2002 was 81% of the 2001 numbers. Wild steelhead trout collection in 2002 was 81% of the previous year's catch. Trap operations began on March 10 and were terminated on May 29 due to high flows. The trap was out of operation for four days due to high flow or debris. The increase in hatchery Chinook catch in 2002 was due to a 3.1 fold increase in hatchery production and differences in flow between years. Changes in hatchery and wild steelhead catch are probably due to differences in flow between years. Travel time (d) and migration rate (km/d) through Lower Granite Reservoir for PIT-tagged Chinook salmon and steelhead trout marked at the Snake River trap were affected by discharge. Statistical analysis of 2002 data detected a relation between migration rate and discharge for hatchery and wild Chinook salmon. For hatchery and wild Chinook salmon there was a 4.7-fold and a 3.7-fold increase in migration rate, respectively, between 50 and 100 kcfs. For steelhead trout tagged at the Snake River trap, statistical analysis detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge. For hatchery and wild steelhead trout, there was a 1.8-fold and a 1.7-fold increase in migration rate, respectively, between 50 and 100 kcfs. Travel time and migration rate to Lower Granite Dam for fish marked at the Salmon River trap were calculated. Statistical analysis of the 2002 data detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge for wild Chinook salmon and hatchery steelhead trout. The analysis was unable to detect a relation between migration rate and discharge for hatchery Chinook salmon. The lack of a detectable relation was probably a result of the migration rate data being spread over a very narrow range of discharge. Not enough data were available to perform the analysis for wild steelhead trout. Migration rate increased 4.3-fold for wild Chinook salmon and 2.2-fold for hatchery steelhead between 50 kcfs and 100 kcfs. Fish tagged with passive integrated transponder (PIT) tags at the Snake River trap were interrogated at four dams with PIT tag detection systems (Lower Granite, Little Goose, Lower Monumental, and McNary dams). Because of the addition of the fourth interrogation site (Lower Monumental) in 1993 and the installation of the Removable Spillway Weir at

  18. Smolt Monitoring at the Head of Lower Granite Reservoir and Lower Granite Dam, 2004 Annual Report.

    SciTech Connect (OSTI)

    Buettner, Edwin W.; Putnam, Scott A.

    2009-02-18

    This project monitored the daily passage of Chinook salmon Oncorhynchus tshawytscha, steelhead trout O. mykiss, and sockeye salmon O. nerka smolts during the 2004 spring out-migration at migrant traps on the Snake River and Salmon River. In 2004 fish management agencies released significant numbers of hatchery Chinook salmon and steelhead trout above Lower Granite Dam that were not marked with a fin clip or coded-wire tag. Generally, these fish were distinguishable from wild fish by the occurrence of fin erosion. Total annual hatchery Chinook salmon catch at the Snake River trap was 1.1 times greater in 2004 than in 2003. The wild Chinook catch was 1.1 times greater than the previous year. Hatchery steelhead trout catch was 1.2 times greater than in 2003. Wild steelhead trout catch was 1.6 times greater than the previous year. The Snake River trap collected 978 age-0 Chinook salmon of unknown rearing. During 2004, the Snake River trap captured 23 hatchery and 18 wild/natural sockeye salmon and 60 coho salmon O. kisutch of unknown rearing. Differences in trap catch between years are due to fluctuations not only in smolt production, but also differences in trap efficiency and duration of trap operation associated with flow. Trap operations began on March 7 and were terminated on June 4. The trap was out of operation for a total of zero days due to mechanical failure or debris. Hatchery Chinook salmon catch at the Salmon River trap was 10.8% less and wild Chinook salmon catch was 19.0% less than in 2003. The hatchery steelhead trout collection in 2004 was 20.0% less and wild steelhead trout collection was 22.3% less than the previous year. Trap operations began on March 7 and were terminated on May 28 due to high flows. There were two days when the trap was taken out of service because wild Chinook catch was very low, hatchery Chinook catch was very high, and the weekly quota of PIT tagged hatchery Chinook had been met. Travel time (d) and migration rate (km/d) through Lower Granite Reservoir for PIT-tagged Chinook salmon and steelhead trout marked at the Snake River trap were affected by discharge. Statistical analysis of 2004 data detected a relation between migration rate and discharge for wild Chinook salmon but was unable to detect a relation for hatchery Chinook. The inability to detect a migration rate discharge relation for hatchery Chinook salmon was caused by age-0 fall Chinook being mixed in with the age 1 Chinook. Age-0 fall Chinook migrate much slower than age-1 Chinook, which would confuse the ability to detect the migration rate discharge relation. When several groups, which consisted of significant numbers of age-0 Chinook salmon, were removed from the analysis a relation was detected. For hatchery and wild Chinook salmon there was a 2.8-fold and a 2.4-fold increase in migration rate, respectively, between 50 and 100 kcfs. For steelhead trout tagged at the Snake River trap, statistical analysis detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge. For hatchery and wild steelhead trout, there was a 2.3-fold and a 2.0-fold increase in migration rate, respectively, between 50 and 100 kcfs. Travel time and migration rate to Lower Granite Dam for fish marked at the Salmon River trap were calculated. Statistical analysis of the 2004 data detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge for hatchery Chinook salmon, wild Chinook salmon and hatchery steelhead trout. Not enough data were available to perform the analysis for wild steelhead trout. Migration rate increased 7.0-fold for hatchery Chinook salmon, 4.7-fold for wild Chinook salmon and 3.8-fold for hatchery steelhead as discharge increased between 50 kcfs and 100 kcfs. Fish tagged with passive integrated transponder (PIT) tags at the Snake River and Salmon River traps were interrogated at four dams with PIT tag detection systems (Lower Granite, Little Goose, Lower Monumental, and McNary dams). Because of the addition of the fourth interrogation site (Lower Monume

  19. Prestack mid-value filtering

    SciTech Connect (OSTI)

    Changlian, X. )

    1992-01-01

    This paper describes mid-value filtering, a specific nonlinear smoothing filtering, and widely used in graphic processing, etc. Mid-value filtering before stack of seismic data can remove wild value (inconceivable particular big value) and improve signal-noise ratio. In view of big data volume before stack, computation efficiency of mid-value filtering is critical to its feasibility. The algorithm used here is sufficiently based on the properties of mid-value filtering, so that the computation efficiency is greatly improved. It is experimentally shown that prestack mid-value filtering can quite well eliminate wild value, abnormal traces as well as surface waves, and raise signal-noise ratio. After lateral low frequency noises are removed by high pass filtering, mid-value filtering works better.

  20. Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells

    SciTech Connect (OSTI)

    Durand, Fabien; Stines-Chaumeil, Claire; Flexer, Victoria; Andre, Isabelle; CNRS, UMR5504, F-31400 Toulouse; INRA, UMR 792 Ingenierie des Systemes Biologiques et des Procedes, F-31400 Toulouse ; Mano, Nicolas

    2010-11-26

    Research highlights: {yields} A new mutant of PQQ-GDH designed for glucose biosensors application. {yields} First mutant of PQQ-GDH with higher activity for D-glucose than the Wild type. {yields} Position N428 is a key point to increase the enzyme activity. {yields} Molecular modeling shows that the N428 C mutant displays a better interaction for PQQ than the WT. -- Abstract: We report for the first time a soluble PQQ-glucose dehydrogenase that is twice more active than the wild type for glucose oxidation and was obtained by combining site directed mutagenesis, modelling and steady-state kinetics. The observed enhancement is attributed to a better interaction between the cofactor and the enzyme leading to a better electron transfer. Electrochemical experiments also demonstrate the superiority of the new mutant for glucose oxidation and make it a promising enzyme for the development of high-performance glucose biosensors and biofuel cells.

  1. Hellsgate Winter Range : Wildlife Mitigation Project. Preliminary Environmental Assessment.

    SciTech Connect (OSTI)

    United States. Bonneville Power Administration.

    1995-01-01

    The Bonneville Power Administration proposes funding the Hellsgate Winter Range Wildlife Mitigation Project in cooperation with the Colville Convederated Tribes and Bureau of Indian Affairs. This Preliminary Environmental Assessment examines the potential environmental effects of acquiring and managing property for wildlife and wildlife habitat within a large project area. The Propose action is intended to meet the need for mitigation of wildlife and wild life habitat that was adversely affected by the construction of Grand Coulee and Chief Joseph Dams and their reservoirs.

  2. Survival Estimates for the Passage of Spring-Migrating Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2005-2006 Annual Report.

    SciTech Connect (OSTI)

    Smith, Steven G.; Muir, William D.; Marsh, Douglas M.

    2006-05-01

    In 2005, the National Marine Fisheries Service and the University of Washington completed the thirteenth year of a study to estimate survival and travel time of juvenile salmonids Oncorhynchus spp. passing through dams and reservoirs on the Snake and Columbia Rivers. All estimates were derived from detections of fish tagged with passive integrated transponder tags (PIT tags). We PIT tagged and released a total of 18,439 hatchery steelhead, 5,315 wild steelhead, and 6,964 wild yearling Chinook salmon at Lower Granite Dam in the Snake River. In addition, we utilized fish PIT tagged by other agencies at traps and hatcheries upstream from the hydropower system and at sites within the hydropower system in both the Snake and Columbia Rivers. PIT-tagged smolts were detected at interrogation facilities at Lower Granite, Little Goose, Lower Monumental, Ice Harbor, McNary, John Day, and Bonneville Dams and in the PIT-tag detector trawl operated in the Columbia River estuary. Survival estimates were calculated using a statistical model for tag-recapture data from single release groups (the ''single-release model''). Primary research objectives in 2005 were: (1) Estimate reach survival and travel time in the Snake and Columbia Rivers throughout the migration period of yearling Chinook salmon O. tshawytscha and steelhead O. mykiss. (2) Evaluate relationships between survival estimates and migration conditions. (3) Evaluate the survival estimation models under prevailing conditions. This report provides reach survival and travel time estimates for 2005 for PIT-tagged yearling Chinook salmon (hatchery and wild), hatchery sockeye salmon O. nerka, hatchery coho salmon O. kisutch, and steelhead (hatchery and wild) in the Snake and Columbia Rivers. Additional details on the methodology and statistical models used are provided in previous reports cited here.

  3. NREL: Wind Research - Wind and Water Power Fact Sheets

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wind and Water Power Fact Sheets The capabilities for research at the National Wind Technology Center (NWTC) are numerous. Below you will find fact sheets about the many facilities and capabilities at the NWTC, including field testing research, modeling and simulation, and the Wind-Wildlife Impacts Literature Database. Fact Sheet Cover 35 Years of Innovation: Leading the Way to a Clean Energy Future Fact Sheet Cover Wind-Wildlife Impacts Literature Database (WILD) Fact Sheet Cover NREL Software

  4. fe0024357-osu | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Resources - Field Labs Utica Shale Energy and Environment Laboratory (USEEL) Last Reviewed 9/9/2015 DE-FE0024357 Goal The goal of this project is to develop and maintain a field research facility in the heart of the Utica Shale play that will provide a platform for environmental and geotechnical studies before, during, and after unconventional oil and gas (UOG) development. Performers The Ohio State University, Columbus, Ohio West Virginia University, Morgantown, West Virginia The Wilds,

  5. U-054: Security Advisory for Adobe Reader and Acrobat

    Broader source: Energy.gov [DOE]

    This U3D memory corruption vulnerability (CVE-2011-2462) could cause a crash and potentially allow an attacker to take control of the affected system. There are reports that the vulnerability is being actively exploited in the wild in limited, targeted attacks against Adobe Reader 9.x on Windows. Adobe Reader X Protected Mode and Acrobat X Protected View mitigations would prevent an exploit of this kind from executing.

  6. SREL Reprint #3285

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 Habitats associated with vehicle collisions with wild pigs James C. Beasley1, Tracy E. Grazia2, Paul E. Johns3, and John J. Mayer4 1Savannah River Ecology Laboratory, PO Drawer E, Aiken, South Carolina 29802, USA 2USDA Forest Service – Savannah River, PO Box 700, New Ellenton, South Carolina 29809, USA 3Carolina Wildlife Consultants, 2441 Williston Road, Aiken, South Carolina 29803, USA 4Savannah River National Laboratory, SRNS LLC, Savannah River Site, Aiken, South Carolina 29808, USA

  7. SREL Reprint #3328

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8 Evaluation of Rhodamine B as a biomarker for assessing bait acceptance in wild pigs James Beasley1, Sarah C. Webster1, Olin E. Rhodes, Jr.1, and Fred L. Cunningham2 1Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA 2United States Department of Agriculture, Animal Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, 125 Stone Boulevard, Scales Building, Mississippi State, MS 39762, USA Abstract: Worldwide, there is

  8. Wall-associated kinase-like polypeptide mediates nutritional status perception and response

    DOE Patents [OSTI]

    Yang, Zhenbiao; Karr, Stephen

    2014-02-11

    The disclosure relates to methods for modulating plant growth and organogenesis using dominant-negative receptor-like kinases. The disclosure further provides a method for increasing plant yield relative to corresponding wild type plants comprising modulating the expression in a plant of a nucleic acid encoding a Wall-Associated Kinase-like 14 polypeptide or a homolog thereof, and selecting for plants having increased yield or growth on a nutrient deficient substrate.

  9. Thermophilic Endoglucanase Enzymes Engineered for Increased Activity -

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Innovation Portal Thermophilic Endoglucanase Enzymes Engineered for Increased Activity Lawrence Berkeley National Laboratory Contact LBL About This Technology Technology Marketing SummaryResearchers at the Joint BioEnergy Institute (JBEI) have generated and identified new enzyme variants of cellulase from the thermophile Thermotoga maritima with catalytic activities significantly higher than naturally existing (wild type) endoglucanases. The JBEI enzymes allow for more efficient

  10. Seeking New Approaches to Investigate Domestication Events | Stanford

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Synchrotron Radiation Lightsource Seeking New Approaches to Investigate Domestication Events Monday, October 29, 2012 - 3:30am SSRL Bldg. 137, Rm. 322 Krish Seetah, Stanford University, Department of Anthropology and Zooarcheology Laboratory The domestication of wild animal species has underpinned some of the most fundamental developments in human history. The inclusion of a range of fauna into the human menagerie has altered the way we feed and transport ourselves, not to mention how we

  11. 'Comic Book Physics' examined at Jefferson Lab's March 25 Science Series

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    event | Jefferson Lab Comic book physics Even superheroes must obey the laws of physics - or do they? Artwork: Kim Dylla 'Comic Book Physics' examined at Jefferson Lab's March 25 Science Series event February 26, 2003 The wild, wacky world of 'Comic Book Physics' will be investigated by guest speaker Jim Kakalios, from the University of Minnesota, at Jefferson Lab's Spring Science Series event, set for Tuesday, March 25, 2003 During this educational, entertaining event, Kakalios posits: Even

  12. Quickstart Resume Template

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    JAMES C. BEASLEY Savannah River Ecology Lab Phone: (803) 725-5113 P.O. Drawer E Fax: (803) 725-3309 Aiken, SC 29802 Email: beasley@srel.uga.edu RESEARCH INTERESTS Spatial ecology and population dynamics of carnivores, effects of anthropogenic stressors on wildlife, wild pig ecology and management, human-wildlife conflicts, wildlife disease ecology, scavenging ecology EDUCATION: Ph.D., Wildlife Ecology, Purdue University 2010 Advisor: Dr. Olin Rhodes, Jr. M.S., Wildlife Ecology, Purdue University

  13. ACCOLADES: A Scalable Workflow Framework for Large-Scale Simulation and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Analyses of Automotive Engines | Argonne National Laboratory ACCOLADES: A Scalable Workflow Framework for Large-Scale Simulation and Analyses of Automotive Engines Title ACCOLADES: A Scalable Workflow Framework for Large-Scale Simulation and Analyses of Automotive Engines Publication Type Conference Proceedings Year of Publication 2015 Authors Aithal, SM, Wild, SM Conference Name High Performance Computing Volume 9137 Pagination 87-95 Publisher Springer Conference Location Frankfurt, Germany

  14. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Significant Decadal Brightening over the Continental United States Download a printable PDF Submitter: Long, C. N., NOAA Global Monitoring Division/CIRES Dutton, E. G., NOAA/OAR/ESRL Augustine, J., National Oceanic and Atmospheric Administration Wiscombe, W. J., Brookhaven National Laboratory Wild, M., Institute for Atmospheric and Climate Science - ETH Zurich McFarlane, S. A., U.S. Department of Energy Flynn, C. J., Pacific Northwest National Laboratory Area of Research: Radiation Processes

  15. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Earth Lightens Up Submitter: Long, C. N., NOAA Global Monitoring Division/CIRES Area of Research: Radiation Processes Working Group(s): Radiative Processes Journal Reference: Wild, M., H. Gilgen, A. Roesch, A. Ohmura, C. N. Long, E. G. Dutton, B. Forgan, A. Kallis, V. Russak, and A. Tsvetkov, (2005): From dimming to brightening: Decadal changes in solar radiation at the Earth's surface, Science, 308, Issue 5723, 847-850, [DOI:10.1126/science.1103215] Global distribution of surface observation

  16. November 12, 2010

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cafés November 12, 2010 November 12, 2010 Print Friday, 12 November 2010 00:00 christine beavers Having a ball in the wild world of fullerenes Christine Beavers, Beamline 11.3.1 nils huse Nanoclimates: Watching electron clouds move Nils Huse, Chemical Sciences, Beamlines 6.0.1/6.0.2 stefano marchesini Stitching x rays Stefano Marchesini, Beamline 9.0

  17. Wetlands, Microbes, and the Carbon Cycle: Behind the Scenes @ Berkeley Lab

    ScienceCinema (OSTI)

    Tringe, Susannah

    2013-05-29

    Susannah Tringe, who leads the Metagenome Program at the Department of Energy's Joint Genome Institute (JGI), a collaboration in which Berkeley Lab plays a leading role, takes us behind the scenes to show how DNA from unknown wild microbes is extracted and analyzed to see what role they play in the carbon cycle. Tringe collects samples of microbial communities living in the wetland muck of the Sacramento-San Joaquin River Delta, organisms that can determine how these wetlands store or release carbon.

  18. Leveraging Tribal Renewable Resources to Support Military Energy Goals |

    Energy Savers [EERE]

    Department of Energy Leveraging Tribal Renewable Resources to Support Military Energy Goals Leveraging Tribal Renewable Resources to Support Military Energy Goals May 30-31, 2013 Image of a speaker at the forum. Chandler, Arizona Wild Horse Pass Hotel and Casino The Office of Indian Energy Tribal Leader Energy Forum on "Leveraging Tribal Renewable Resources to Support Military Energy Goals" was held May 30-31, 2013, in Chandler, Arizona. The forum provided information for western

  19. Wetlands, Microbes, and the Carbon Cycle: Behind the Scenes @ Berkeley Lab

    SciTech Connect (OSTI)

    Tringe, Susannah

    2012-01-01

    Susannah Tringe, who leads the Metagenome Program at the Department of Energy's Joint Genome Institute (JGI), a collaboration in which Berkeley Lab plays a leading role, takes us behind the scenes to show how DNA from unknown wild microbes is extracted and analyzed to see what role they play in the carbon cycle. Tringe collects samples of microbial communities living in the wetland muck of the Sacramento-San Joaquin River Delta, organisms that can determine how these wetlands store or release carbon.

  20. Derivative-free optimization for parameter estimation in computational

    Office of Scientific and Technical Information (OSTI)

    nuclear physics (Journal Article) | SciTech Connect Journal Article: Derivative-free optimization for parameter estimation in computational nuclear physics Citation Details In-Document Search Title: Derivative-free optimization for parameter estimation in computational nuclear physics Authors: Wild, S ; Sarich, J ; Schunck, N Publication Date: 2014-06-20 OSTI Identifier: 1177246 Report Number(s): LLNL-JRNL-656057 DOE Contract Number: DE-AC52-07NA27344 Resource Type: Journal Article Resource

  1. Fifteenmile Basin Habitat Enhancement Project: Annual Report FY 1990.

    Office of Scientific and Technical Information (OSTI)

    (Technical Report) | SciTech Connect Technical Report: Fifteenmile Basin Habitat Enhancement Project: Annual Report FY 1990. Citation Details In-Document Search Title: Fifteenmile Basin Habitat Enhancement Project: Annual Report FY 1990. The goal of the Fifteenmile Creek Habitat Improvement project is to improve wild winter steelhead habitat in the Fifteenmile Creek Basin. This goal was addressed under the Columbia River Basin Fish and Wildlife Program, Measure 703 (c) (1) - Action Item 4.2.

  2. UCRL-ID-120738 L-20072-1 NIF System-Design Requirements for

    Office of Scientific and Technical Information (OSTI)

    0738 L-20072-1 NIF System-Design Requirements for Nuclear-Weapons Physics Experiments Theodore S. Perry Lawrence Livermore National Laboratory and Bernhard H. Wilde Los Alamos National Laboratory Scientific Editors April 1995 LAWRENCE LIVERMORE NATIONAL LABORATORY University of California Livermore, California 94550 I DISCLAIMER This document was prepared as an account of work sponsored by an agency o f the United States Government. Neither the United States Government nor the University of

  3. hagan-99.PDF

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Compact Spectral Radiometer for In Situ Solar Flux Measurements D. E. Hagan, M. Foote, J.-F. Blavier, L. Wild, and E. Jones Jet Propulsion Laboratory California Institute of Technology Pasadena, California Introduction We describe here the breadboard version of a lightweight, compact radiometer, which uses microthermopile detectors for hemispheric measurements of broadband and spectral downwelling solar irradiance. The instrument has undergone initial operability tests, and some limited field

  4. Hanford For Students and Kids - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hanford For Students and Kids Hanford For Students and Kids Hanford Fun Facts Classroom Projects Famous People of Hanford Hanford For Students and Kids Email Email Page | Print Print Page |Text Increase Font Size Decrease Font Size The Hanford Nuclear Reservation is a pretty amazing place. Located in the present-day southeast corner of Washington State, the area where Hanford sits has a history that actually began thousands of years ago when wild animals roamed free and Indians were the only

  5. NERSCbench_NUG_final.ppt

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science Driven System Architectures (and a few words about benchmarking) John Shalf NERSC Users Group Meeting Princeton Plasma Physics Laboratory June 2006 Office of Science U.S. Department of Energy Overview  Overview of the SSP for procurement benchmarks  Recent Benchmarking Activities  Workload Characterization  Wild Ideas Office of Science U.S. Department of Energy NERSC Mission  Support large scale computational science that cannot be done elsewhere  Support wide variety

  6. Projects | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Projects bgclang Compiler Hal Finkel Cobalt Scheduler Bill Allcock, Paul Rich, Brian Toonen, Tom Uram GLEAN: Scalable In Situ Analysis and I/O Acceleration on Leadership Computing Systems Michael E. Papka, Venkat Vishwanath, Mark Hereld, Preeti Malakar, Joe Insley, Silvio Rizzi, Tom Uram Petrel: Data Management and Sharing Pilot Ian Foster, Michael E. Papka, Bill Allcock, Ben Allen, Rachana Ananthakrishnan, Lukasz Lacinski The Swift Parallel Scripting Language for ALCF Systems Michael Wilde,

  7. Modified Yeast Ferments Biomass Xylose - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ferments Biomass Xylose Great Lakes Bioenergy Research Center Contact GLBRC About This Technology Technology Marketing Summary Producing biofuel on a useful scale requires efficient fermentation of cellulosic plant material. The sugars glucose and xylose are the most abundant carbohydrates found in hemicellulose. The yeast most commonly utilized for industrial fermentation - Saccharomyces cerevisiae - can ferment glucose but not xylose. By studying the genomes of wild strains of yeast capable of

  8. Microsoft Word - 02 Tilghman.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    6 January 2016 The Wild and Wacky World of Epigenetics Shirley M. Tilghman Princeton University Princeton, New Jersey ABSTRACT: If Gregor Mendel, the 19 th century father of modern genetics, were alive today, his head would be spinning. The simple rules of "Mendelian" inheritance, which he painstakingly worked out with garden peas, have had to be substantially expanded and revised in the last several decades to accommodate the fact that genes are not nearly as well behaved as he

  9. Slide 1

    Energy Savers [EERE]

    for Military Applications Leveraging Tribal Renewable Energy Resources to Support Military Energy Goals May 31-31, 2013 Wild Horse Pass Chandler, AZ Sacred Power Corporation Sacred Power Products 6/24/2013 Sacred Power Corporation Sacred Power Corporation?  Longevity Oldest Native American Solar Company  Diversity Manufacturing & Installation  Experience Over 100 years combined  Products 3 Patented Products  Ethnicity Native American Owned  Reputation Established

  10. T-638: Security update available for Adobe Flash Player

    Broader source: Energy.gov [DOE]

    This universal cross-site scripting vulnerability (CVE-2011-2107) could be used to take actions on a user's behalf on any website or webmail provider, if the user visits a malicious website. There are reports that this vulnerability is being exploited in the wild in active targeted attacks designed to trick the user into clicking on a malicious link delivered in an email message.

  11. Frances Beinecke | Department of Energy

    Office of Environmental Management (EM)

    Frances Beinecke About Us Frances Beinecke - President of Natural Resources Defense Council Photo of Frances Beinecke Frances Beinecke is the president of NRDC. Under Ms. Beinecke's leadership, the organization has launched a new strategic campaign that sharply focuses NRDC's efforts on establishing a clean energy future that curbs climate change, reviving the world's oceans, defending endangered wildlife and wild places, protecting our health by preventing pollution, fostering sustainable

  12. Joint Final Environmental Impact Statement Montanore Project

    Office of Environmental Management (EM)

    2 Chapter 3: Affected Environment and Environmental Consequences Section 3.7, Cultural Resources through Section 3.24, Wilderness, Roadless Areas, and Wild and Scenic Rivers United States Department of Agriculture Forest Service Northern Region Kootenai National Forest Montana Department of Environmental Quality The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable,

  13. INDIAN COUNTRY ENERGY AND INFRASTRUCTURE WORKING GROUP ICEIWG

    Office of Environmental Management (EM)

    8 INDIAN COUNTRY ENERGY AND INFRASTRUCTURE WORKING GROUP ICEIWG May 29, 2013 Wild Horse Pass Hotel and Casino Chandler, Arizona MEETING OVERVIEW The U.S. Department of Energy Office (DOE) of Indian Energy (IE) hosted an Indian Country Energy and Infrastructure Working Group (ICEIWG) Meeting on Wednesday, May 29, 2013 in Chandler, Arizona. IE welcomed reappointed and new members to ICEIWG. The ICEIWG meeting was held prior to the seventh Tribal Leader Forum on "Leveraging Tribal Renewable

  14. Menominee Tribal Enterprises

    Office of Environmental Management (EM)

    The Menominee Nation once occupied 9.5 million acres of land * The present Menominee Reservation was established in 1854 with 234,000 acres. * The Menominee hunted, fished and harvested wild rice on their reservation. MTE Menominee Tribal Enterprises The Menominee Nation * The Menominee retained their island of timber & community by dint of their own tenacity, federal protection and fortuitous circumstance. MTE Menominee Tribal Enterprises The Menominee Nation * 95% of the reservation

  15. Menominee Tribal Enterprises (MTE) Biomass CHP District Energy System

    Office of Environmental Management (EM)

    The Menominee Nation once occupied 9.5 million acres of land * The present Menominee Reservation was established in 1854 with 234,000 acres. * The Menominee hunted, fished and harvested wild rice on their reservation. MTE Menominee Tribal Enterprises The Menominee Nation * The Menominee retained their island of timber & community by perseverance of their own tenacity, federal protection and fortuitous circumstance. MTE Menominee Tribal Enterprises The Menominee Nation * 95% of the

  16. Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum

    SciTech Connect (OSTI)

    Lynd, Lee R; Shao, Xiongjun; Raman, Babu; Mielenz, Jonathan R; Brown, Steven D; Guss, Adam M; Zhu, Mingjun

    2011-01-01

    Clostridium thermocellum is a model microorganism for converting cellulosic biomass into fuels and chemicals via consolidated bioprocessing. One of the challenges for industrial application of this organism is its low ethanol tolerance, typically 1 2% (w/v) in wild-type strains. In this study, we report the development and characterization of mutant C. thermocellum strains that can grow in the presence of high ethanol concentrations. Starting from a single colony, wild-type C. thermocellum ATCC 27405 was sub-cultured and adapted for growth in up to 50 g/L ethanol using either cellobiose or crystalline cellulose as the growth substrate. Both the adapted strains retained their ability to grow on either substrate and displayed a higher growth rate and biomass yield than the wild-type strain in the absence of ethanol. With added ethanol in the media, the mutant strains displayed an inverse correlation between ethanol concentration and growth rate or biomass yield. Genome sequencing revealed six common mutations in the two ethanol-tolerant strains including an alcohol dehydrogenase gene and genes involved in arginine/pyrimidine biosynthetic pathway. The potential role of these mutations in ethanol tolerance phenotype is discussed.

  17. Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum

    SciTech Connect (OSTI)

    Shao, Xiongjun; Raman, Babu; Zhu, Mingjun; Mielenz, Jonathan R; Brown, Steven D; Guss, Adam M; Lynd, Lee R

    2011-01-01

    Clostridium thermocellum is a model microorganism for converting cellulosic biomass into fuels and chemicals via consolidated bioprocessing. One of the challenges for industrial application of this organism is its low ethanol tolerance, typically 1-2% (w/v) in wild-type strains. In this study, we report the development and characterization of mutant C. thermocellum strains that can grow in the presence of high ethanol concentrations. Starting from a single colony, wild-type C. thermocellum ATCC 27405 was sub-cultured and adapted for growth in up to 50 g/L ethanol using either cellobiose or crystalline cellulose as the growth substrate. Both the adapted strains retained their ability to grow on either substrate and displayed a higher growth rate and biomass yield than the wild-type strain in the absence of ethanol. With added ethanol in the media, the mutant strains displayed an inverse correlation between ethanol concentration and growth rate or biomass yield. Genome sequencing revealed six common mutations in the two ethanol-tolerant strains including an alcohol dehydrogenase gene and genes involved in arginine/pyrimidine biosynthetic pathway. The potential role of these mutations in ethanol tolerance phenotype is discussed.

  18. Hood River and Pelton Ladder Evaluation Studies, 2008 Annual Report : October 2007 - September 2008.

    SciTech Connect (OSTI)

    Reagan, Robert E.; Olsen, Erik A.

    2009-09-28

    This report summarizes the life history and production data collected in the Hood River subbasin during FY 2008. Included is a summary of jack and adult life history data collected at the Powerdale Dam trap on seventeen complete run years of winter steelhead, spring and fall chinook salmon, and coho salmon, and on fifteen complete run years of summer steelhead. Also included are summaries of (1) the hatchery winter steelhead broodstock collection program; (2) hatchery production releases in the Hood River subbasin; (3) subbasin wild summer and winter steelhead smolt production, (4) numbers of hatchery summer and winter steelhead smolts leaving the subbasin; (5) smolt migration timing past Bonneville Dam, (6) wild and hatchery steelhead smolt-to-adult survival rates; (7) wild summer and winter steelhead egg to smolt survival rates; and (8) streamflow at selected locations in the Hood River subbasin. Data will be used in part to (1) evaluate the HRPP relative to its impact on indigenous populations of resident and anadromous salmonids (see Ardren Draft), (2) evaluate the HRPP's progress towards achieving the biological fish objectives defined in the Hood River Subbasin Plan (Coccoli 2004) and the Revised Master Plan for the Hood River Production Program (HDR|FishPro, ODFW, and CTWSRO 2008), (3) refine spawner escapement objectives to more accurately reflect subbasin carrying capacity, and (4) refine estimates of subbasin smolt production capacity to more accurately reflect current and potential subbasin carrying capacity.

  19. Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis

    SciTech Connect (OSTI)

    Pauly, Markus; Sorensen, Susanne Oxenboll; Harholt, Jesper; Geshi, Naomi; Sakuragi, Yumiko; Moller, Isabel; Zandleven, Joris; Bernal, Adriana J.; Jensen, Niels Bjerg; Sorensen, Charlotte; Jensen, Jacob K.; Beldman, Gerrit; Willats, William G.T.; Scheller, Henrik

    2009-08-19

    Xylogalacturonan (XGA) is a class of pectic polysaccharide found in plant cell walls. The Arabidopsis thaliana locus At5g33290 encodes a predicted Type II membrane protein, and insertion mutants of the At5g33290 locus had decreased cell wall xylose. Immunological studies, enzymatic extraction of polysaccharides, monosaccharide linkage analysis, and oligosaccharide mass profiling were employed to identify the affected cell wall polymer. Pectic XGA was reduced to much lower levels in mutant than in wild-type leaves, indicating a role of At5g33290 in XGA biosynthesis. The mutated gene was designated xylogalacturonan deficient1 (xgd1). Transformation of the xgd1-1 mutant with the wild-type gene restored XGA to wild-type levels. XGD1 protein heterologously expressed in Nicotiana benthamiana catalyzed the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. The products formed could be hydrolyzed with an XGA-specific hydrolase. These results confirm that the XGD1 protein is a XGA xylosyltransferase. The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis.

  20. Smolt Monitoring at the Head of Lower Granite Reservoir and Lower Granite Dam, 2003 Annual Report.

    SciTech Connect (OSTI)

    Buettner, Edwin W.; Putnam, Scott A.

    2009-02-18

    This project monitored the daily passage of Chinook salmon Oncorhynchus tshawytscha, steelhead trout O. mykiss, and sockeye salmon O. nerka smolts during the 2003 spring out-migration at migrant traps on the Snake River and Salmon River. In 2003 fish management agencies released significant numbers of hatchery Chinook salmon and steelhead trout above Lower Granite Dam that were not marked with a fin clip or coded-wire tag. Generally, these fish were distinguishable from wild fish by the occurrence of fin erosion. Total annual hatchery Chinook salmon catch at the Snake River trap was 2.1 times less in 2003 than in 2002. The wild Chinook catch was 1.1 times less than the previous year. Hatchery steelhead trout catch was 1.7 times less than in 2002. Wild steelhead trout catch was 2.1 times less than the previous year. The Snake River trap collected 579 age-0 Chinook salmon of unknown rearing. During 2003, the Snake River trap captured five hatchery and 13 wild/natural sockeye salmon and 36 coho salmon O. kisutch of unknown rearing. Differences in trap catch between years are due to fluctuations not only in smolt production, but also differences in trap efficiency and duration of trap operation associated with flow. The significant differences in catch between 2003 and the previous year were due mainly to low flows during much of the trapping season and then very high flows at the end of the season, which terminated the trapping season 12 days earlier than in 2002. Trap operations began on March 9 and were terminated on May 27. The trap was out of operation for a total of zero days due to mechanical failure or debris. Hatchery Chinook salmon catch at the Salmon River trap was 16.8% less and wild Chinook salmon catch was 1.7 times greater than in 2002. The hatchery steelhead trout collection in 2003 was 5.6% less than in 2002. Wild steelhead trout collection was 19.2% less than the previous year. Trap operations began on March 9 and were terminated on May 24 due to high flows. There were zero days when the trap was out of operation due to high flow or debris. The decrease in hatchery Chinook catch in 2003 was partially due to differences in flow between years because there was a 5.9% increase in hatchery production in the Salmon River drainage in 2003. The decrease in hatchery steelhead catch may be partially due to a 13% decrease in hatchery production in the Salmon River drainage in 2003. Travel time (d) and migration rate (km/d) through Lower Granite Reservoir for PIT-tagged Chinook salmon and steelhead trout marked at the Snake River trap were affected by discharge. Statistical analysis of 2003 data detected a relation between migration rate and discharge for wild Chinook salmon but was unable to detect a relation for hatchery Chinook. The inability to detect a migration rate discharge relation for hatchery Chinook was probably caused by age 0 fall Chinook being mixed in with the age 1 Chinook. Age 0 fall Chinook migrate much slower than age 1 Chinook, which would confuse the ability to detect the migration rate discharge relation. For wild Chinook salmon there was a 1.4-fold increase in migration rate, respectively, between 50 and 100 kcfs. For steelhead trout tagged at the Snake River trap, statistical analysis detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge. For hatchery and wild steelhead trout, there was a 1.7-fold and a 1.9-fold increase in migration rate, respectively, between 50 and 100 kcfs. Travel time and migration rate to Lower Granite Dam for fish marked at the Salmon River trap were calculated. Statistical analysis of the 2003 data detected a significant relation between migration rate and Lower Granite Reservoir inflow discharge for hatchery Chinook salmon, wild Chinook salmon and hatchery steelhead trout. Not enough data were available to perform the analysis for wild steelhead trout. Migration rate increased 14-fold for hatchery Chinook salmon, 8.3-fold for wild Chinook salmon and 2.4-fold for hatchery steelhead as discharge increased between 50 kcfs and

  1. Metabonomic Profiling of TASTPM Transgenic Alzheimer's Disease Mouse Model

    SciTech Connect (OSTI)

    Hu, Zeping; Browne, Edward R.; Liu, Tao; Angel, Thomas E.; Ho, Paul C.; Chun Yong Chan, Eric

    2012-12-07

    Identification of molecular mechanisms underlying early stage Alzheimer’s disease (AD) is important for the development of new therapies against and diagnosis of AD. In this study, non-targeted metabotyping of TASTPM transgenic AD mice was performed. The metabolic profiles of both brain and plasma of TASTPM mice were characterized using gas chromatography-mass spectrometry and compared to those of wild type C57BL/6J mice. TASTPM mice were metabolically distinct compared to wild type mice (Q28 Y = 0.587 and 0.766 for PLS-DA models derived from brain and plasma, respectively). A number of metabolites were found to be perturbed in TASTPM mice in both brain (D11 fructose, L-valine, L-serine, L-threonine, zymosterol) and plasma (D-glucose, D12 galactose, linoleic acid, arachidonic acid, palmitic acid and D-gluconic acid). In addition, enzyme immunoassay confirmed that selected endogenous steroids were significantly perturbed in brain (androstenedione and 17-OH-progesterone) and plasma (cortisol and testosterone) of TASTPM mice. Ingenuity pathway analysis revealed that perturbations related to amino acid metabolism (brain), steroid biosynthesis (brain), linoleic acid metabolism (plasma) and energy metabolism (plasma) accounted for the differentiation of TASTPM and wild-type

  2. Resistance to collagen-induced arthritis in SHPS-1 mutant mice

    SciTech Connect (OSTI)

    Okuzawa, Chie; Kaneko, Yoriaki; Murata, Yoji; Miyake, Astuko; Saito, Yasuyuki; Okajo, Jun; Tomizawa, Takeshi; Kaneko, Yuka; Okazawa, Hideki; Ohnishi, Hiroshi; Matozaki, Takashi Nojima, Yoshihisa

    2008-07-04

    SHPS-1 is a transmembrane protein that binds the protein tyrosine phosphatases SHP-1 and SHP-2 through its cytoplasmic region and is abundantly expressed on dendritic cells and macrophages. Here we show that mice expressing a mutant form of SHPS-1 fail to develop type-II collagen (CII)-induced arthritis (CIA), a model for rheumatoid arthritis in humans. Histological examinations of the arthritic paws from immunized wild-type mice revealed that cartilage was destroyed in association with marked mononuclear cell infiltration, while only mild cell infiltration was observed in immunized SHPS-1 mutant mice. Consistently, the serum levels of both IgG and IgG2a specific to CII and of IL-1{beta} in immunized SHPS-1 mutant mice were markedly reduced compared with those apparent for wild-type mice. The CII-induced proliferation of, and production of cytokines by, T cells from immunized SHPS-1 mutant mice were reduced compared to wild-type cells. These results suggest that SHPS-1 is essential for development of CIA.

  3. NOVA Making Stuff Season 2

    SciTech Connect (OSTI)

    Leombruni, Lisa; Paulsen, Christine Andrews

    2014-12-12

    Over the course of four weeks in fall 2013, 11.7 million Americans tuned in to PBS to follow host David Pogue as he led them in search of engineering and scientific breakthroughs poised to change our world. Levitating trains, quantum computers, robotic bees, and bomb-detecting plantsthese were just a few of the cutting-edge innovations brought into the living rooms of families across the country in NOVAs four-part series, Making Stuff: Faster, Wilder, Colder, and Safer. Each of the four one-hour programs gave viewers a behind-the-scenes look at novel technologies poised to change our worldshowing them how basic research and scientific discovery can hold the keys to transforming how we live. Making Stuff Season 2 (MS2) combined true entertainment with educational value, creating a popular and engaging series that brought accessible science into the homes of millions. NOVAs goal to engage the public with such technological innovation and basic research extended beyond the broadcast series, including a variety of online, educational, and promotional activities: original online science reporting, web-only short-form videos, a new online quiz-game, social media engagement and promotion, an educational outreach toolkit for science educators to create their own makerspaces, an online community of practice, a series of nationwide Innovation Cafs, educator professional development, a suite of teacher resources, an Idealab, participation in national conferences, and specialized station relation and marketing. A summative evaluation of the MS2 project indicates that overall, these activities helped make a significant impact on the viewers, users, and participants that NOVA reached. The final evaluation conducted by Concord Evaluation Group (CEG) confidently concluded that the broadcast, website, and outreach activities were successful at achieving the projects intended impacts. CEG reported that the MS2 series and website content were successful in raising awareness and sparking interest in innovation, and increased public awareness that basic research leads to technological innovation; this interest was also sustained over a six month period. Efforts to create an online community of practice were also successful: the quality of collaboration increased, and community members felt supported while using Maker pedagogy. These findings provide clear evidence that large-scale science media projects like MS2 are an effective means of moving the needle on attitudes about and excitement for science. NOVAs broadcast audience and ratings have always indicated that a large portion of the population is interested in and engages with educational science media on a weekly basis. Yet these evaluation results provide the empirical evidence that beyond being capable of attracting, maintaining, and growing a dedicated group of citizens interested in science, these showswith their diverse content provided on a variety of media channelsare capable of sparking new interest in science, raising public awareness of the importance of science, and maintaining and growing that interest over time. In a country where approximately a quarter of the population doesnt know the earth rotates around the sun,1 roughly half still dont accept evolution,2 and about 20% dont think climate change is happening,3 the importance of these findings cannot be overstated. The success of MS2 suggests that large-scale media projects dedicated to and linked by coverage of scientific big ideas are an effective means of shifting public opinion onand improving understanding ofscience. REFERENCES 1, 2 National Science Foundation, Science and Engineering Indicators (2014). Chapter 7: Science and Technology: Public Attitudes and Understanding. 3 Leiserowitz, A., Maibach, E., Roser-Renouf, C., Feinberg, G., & Rosenthal, S. (2014) Climate change in the American mind: April, 2014. Yale University and George Mason University. New Haven, CT: Yale Project on Climate Change Communication.

  4. Chief Joseph Kokanee Enhancement Project : Confederated Tribes of the Colville Reservaton 1997 Annual Report.

    SciTech Connect (OSTI)

    LeCaire, Richard

    1998-01-01

    In the early 1980's the Confederated Tribes of the Colville Reservation, the Spokane Tribe of Indians and the Washington Dept. of Fish and Wildlife developed a management plan for Lake Roosevelt on the restoration and enhancement of kokanee salmon populations using hatchery out plants and the restoration of natural spawning runs. The plan was incorporated into the Northwest Power Planning Council (NPPC) in their 1987 Columbia Basin Fish and Wildlife program as partial mitigation for hydropower caused fish losses resulting from the construction of Grand Coulee Dam. The Chief Joseph Kokanee Enhancement Project, as part of a basin wide effort, is evaluating the status of the natural production kokanee in streams tributary to Lakes Roosevelt and Rufus Woods and is examining entrainment through Grand Coulee Dam. The goal of this project is the protection and enhancement of the natural production kokanee in these two lakes. The project is currently collecting data under four phases or parts. Since 1991, Lake Whatcom Washington origin kokanee have been planted in considerable numbers into the waters of Lake Roosevelt. A natural production kokanee fishery has persisted in the lake since the early 1970's(Cash, 1995), (Scholz, 1991). Historical information alludes to wild Kokanee production in the San Poil River, Nespelem River, Big Sheep Creek, Ora-Pa-Ken Creek, Deep Creek and Onion Creeks. The genetic makeup of the fish within the fishery is unknown, as is their contribution to the fishery. The level of influence by the hatchery out planted stock on wild fish stocks is unknown as well. Project outcomes will indicate the genetic fitness for inclusion of natural production kokanee stocks into current Bonneville Power Administration funded hatchery programs. Other findings may determine contribution/interaction of/between wild/hatchery kokanee stocks found in the waters of Lake Franklin D. Roosevelt.

  5. A semisynthetic strategy leads to alteration of the backbone amidate ligand in the NiSOD active site

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Campeciño, Julius O.; Dudycz, Lech W.; Tumelty, David; Berg, Volker; Cabelli, Diane E.; Maroney, Michael J.

    2015-07-01

    Computational investigations have implicated the amidate ligand in nickel superoxide dismutase (NiSOD) in stabilizing Ni-centered redox catalysis and in preventing cysteine thiolate ligand oxidation. To test these predictions, we have used an experimental approach utilizing a semisynthetic scheme that employs native chemical ligation of a pentapeptide (HCDLP) to recombinant S. coelicolor NiSOD lacking these N-terminal residues, NΔ5-NiSOD. Wild-type enzyme produced in this manner exhibits the characteristic spectral properties of recombinant WT-NiSOD and is as catalytically active. The semisynthetic scheme was also employed to construct a variant where the amidate ligand was converted to a secondary amine, H1*-NiSOD, a novel strategymore » that retains a backbone N-donor atom. The H1*-NiSOD variant was found to have only ~1% of the catalytic activity of the recombinant wild-type enzyme, and had altered spectroscopic properties. X-ray absorption spectroscopy reveals a four-coordinate planar site with N2S2-donor ligands, consistent with electronic absorption spectroscopic results indicating that the Ni center in H1*-NiSOD is mostly reduced in the as-isolated sample, as opposed to 50:50 Ni(II)/Ni(III) mixture that is typical for the recombinant wild-type enzyme. The EPR spectrum of as-isolated H1*-NiSOD accounts for ~11% of the Ni in the sample and is similar to WT-NiSOD, but more axial, with gz < gx,y. 14N-hyperfine is observed on gz« less

  6. Down-regulation of gibberellic acid in poplar has negligible effects on host-plant suitability and insect pest response

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Buhl, Christine; Strauss, Steven H.; Lindroth, Richard L.

    2015-01-06

    Abstract Endogenous levels and signaling of gibberellin plant hormones such as gibberellic acid (GA) have been genetically down-regulated to create semi-dwarf varieties of poplar. The potential benefits of semi-dwarf stature include reduced risk of wind damage, improved stress tolerance, and improved wood quality. Despite these benefits, modification of growth traits may have consequences for non-target traits that confer defense against insect herbivores. According to the growth-differentiation balance hypothesis, reductions in growth may shift allocation of carbon from growth to chemical resistance traits, thereby altering plant defense. To date, host-plant suitability and pest response have not been comprehensively evaluated in GAmore » down-regulated plants. We quantified chemical resistance and nitrogen (an index of protein) in GA down-regulated and wild-type poplar (Populus alba × P. tremula) genotypes. We also evaluated performance of both generalist (Lymantria dispar) and specialist (Chrysomela scripta) insect pests reared on these genotypes. Our evaluation of resistance traits in four GA down-regulated genotypes revealed increased phenolic glycosides in one modified genotype and reduced lignin in two modified genotypes relative to the non-transgenic wild type. Nitrogen levels did not vary significantly among the experimental genotypes. Generalists reared on the four GA down-regulated genotypes exhibited reduced performance on only one modified genotype relative to the wild type. Specialists, however, performed similarly across all genotypes. Results from this study indicate that although some non-target traits varied among GA down-regulated genotypes, the differences in poplar pest susceptibility were modest and highly genotype-specific.« less

  7. Crystal Structure of a Super Leucine Zipper an Extended Two-Stranded Super Long Coiled Coil

    SciTech Connect (OSTI)

    J Diao

    2011-12-31

    Coiled coil is a ubiquitous structural motif in proteins, with two to seven alpha helices coiled together like the strands of a rope, and coiled coil folding and assembly is not completely understood. A GCN4 leucine zipper mutant with four mutations of K3A, D7A, Y17W, and H18N has been designed, and the crystal structure has been determined at 1.6 {angstrom} resolution. The peptide monomer shows a helix trunk with short curved N- and C-termini. In the crystal, two monomers cross in 35{sup o} and form an X-shaped dimer, and each X-shaped dimer is welded into the next one through sticky hydrophobic ends, thus forming an extended two-stranded, parallel, super long coiled coil rather than a discrete, two-helix coiled coil of the wild-type GCN4 leucine zipper. Leucine residues appear at every seventh position in the super long coiled coil, suggesting that it is an extended super leucine zipper. Compared to the wild-type leucine zipper, the N-terminus of the mutant has a dramatic conformational change and the C-terminus has one more residue Glu 32 determined. The mutant X-shaped dimer has a large crossing angle of 35{sup o} instead of 18{sup o} in the wild-type dimer. The results show a novel assembly mode and oligomeric state of coiled coil, and demonstrate that mutations may affect folding and assembly of the overall coiled coil. Analysis of the formation mechanism of the super long coiled coil may help understand and design self-assembling protein fibers.

  8. The Phenazine 2-Hydroxy-Phenazine-1-Carboxylic Acid Promotes Extracellular DNA Release and Has Broad Transcriptomic Consequences in Pseudomonas chlororaphis 30–84

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wang, Dongping; Yu, Jun Myoung; Dorosky, Robert J.; Pierson, Leland S.; Pierson, Elizabeth A.

    2016-01-26

    Enhanced production of 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA) by the biological control strain Pseudomonas chlororaphis 30–84 derivative 30-84O* was shown previously to promote cell adhesion and alter the three-dimensional structure of surfaceattached biofilms compared to the wild type. The current study demonstrates that production of 2-OH-PCA promotes the release of extracellular DNA, which is correlated with the production of structured biofilm matrix. Moreover, the essential role of the extracellular DNA in maintaining the mass and structure of the 30–84 biofilm matrix is demonstrated. To better understand the role of different phenazines in biofilm matrix production and gene expression, transcriptomic analyses were conductedmore » comparing gene expression patterns of populations of wild type, 30-84O* and a derivative of 30–84 producing only PCA (30-84PCA) to a phenazine defective mutant (30-84ZN) when grown in static cultures. RNA-Seq analyses identified a group of 802 genes that were differentially expressed by the phenazine producing derivatives compared to 30-84ZN, including 240 genes shared by the two 2-OH-PCA producing derivatives, the wild type and 30-84O*. A gene cluster encoding a bacteriophage- derived pyocin and its lysis cassette was upregulated in 2-OH-PCA producing derivatives. A holin encoded in this gene cluster was found to contribute to the release of eDNA in 30–84 biofilm matrices, demonstrating that the influence of 2-OH-PCA on eDNA production is due in part to cell autolysis as a result of pyocin production and release. The results expand the current understanding of the functions different phenazines play in the survival of bacteria in biofilm-forming communities.« less

  9. A semisynthetic strategy leads to alteration of the backbone amidate ligand in the NiSOD active site

    SciTech Connect (OSTI)

    Campecio, Julius O.; Dudycz, Lech W.; Tumelty, David; Berg, Volker; Cabelli, Diane E.; Maroney, Michael J.

    2015-07-01

    Computational investigations have implicated the amidate ligand in nickel superoxide dismutase (NiSOD) in stabilizing Ni-centered redox catalysis and in preventing cysteine thiolate ligand oxidation. To test these predictions, we have used an experimental approach utilizing a semisynthetic scheme that employs native chemical ligation of a pentapeptide (HCDLP) to recombinant S. coelicolor NiSOD lacking these N-terminal residues, N?5-NiSOD. Wild-type enzyme produced in this manner exhibits the characteristic spectral properties of recombinant WT-NiSOD and is as catalytically active. The semisynthetic scheme was also employed to construct a variant where the amidate ligand was converted to a secondary amine, H1*-NiSOD, a novel strategy that retains a backbone N-donor atom. The H1*-NiSOD variant was found to have only ~1% of the catalytic activity of the recombinant wild-type enzyme, and had altered spectroscopic properties. X-ray absorption spectroscopy reveals a four-coordinate planar site with N2S2-donor ligands, consistent with electronic absorption spectroscopic results indicating that the Ni center in H1*-NiSOD is mostly reduced in the as-isolated sample, as opposed to 50:50 Ni(II)/Ni(III) mixture that is typical for the recombinant wild-type enzyme. The EPR spectrum of as-isolated H1*-NiSOD accounts for ~11% of the Ni in the sample and is similar to WT-NiSOD, but more axial, with gz < gx,y. 14N-hyperfine is observed on gz

  10. Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol yield from cellulose and switchgrass fermentations

    SciTech Connect (OSTI)

    Li, Yongchao; Tschaplinski, Timothy J; Engle, Nancy L; Hamilton, Choo Yieng; Rodriguez, Jr., Miguel; Liao, James C; Schadt, Christopher Warren; Guss, Adam M; Yang, Yunfeng; Graham, David E

    2012-01-01

    Background: The model bacterium Clostridium cellulolyticum efficiently hydrolyzes crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. Although it imports and ferments both pentose and hexose sugars to produce a mixture of ethanol, acetate, lactate, H2 and CO2, the proportion of ethanol is low, which impedes its use in consolidated bioprocessing for biofuels. Therefore genetic engineering will likely be required to improve the ethanol yield. Random mutagenesis, plasmid transformation, and heterologous expression systems have previously been developed for C. cellulolyticum, but targeted mutagenesis has not been reported for this organism. Results: The first targeted gene inactivation system was developed for C. cellulolyticum, based on a mobile group II intron originating from the Lactococcus lactis L1.LtrB intron. This markerless mutagenesis system was used to disrupt both the paralogous L-lactate dehydrogenase (Ccel_2485; ldh) and L-malate dehydrogenase (Ccel_0137; mdh) genes, distinguishing the overlapping substrate specificities of these enzymes. Both mutations were then combined in a single strain. This double mutant produced 8.5-times more ethanol than wild-type cells growing on crystalline cellulose. Ethanol constituted 93% of the major fermentation products (by molarity), corresponding to a molar ratio of ethanol to organic acids of 15, versus 0.18 in wild-type cells. During growth on acid-pretreated switchgrass, the double mutant also produced four-times as much ethanol as wild-type cells. Detailed metabolomic analyses identified increased flux through the oxidative branch of the mutant s TCA pathway. Conclusions: The efficient intron-based gene inactivation system produced the first gene-targeted mutations in C. cellulolyticum. As a key component of the genetic toolbox for this bacterium, markerless targeted mutagenesis enables functional genomic research in C. cellulolyticum and rapid genetic engineering to significantly alter the mixture of fermentation products. The initial application of this system successfully engineered a strain with high ethanol productivity from complex biomass substrates.

  11. KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1

    SciTech Connect (OSTI)

    Goettel, Jeremy A.; Liang, Dongchun; Hilliard, Valda C.; Edelblum, Karen L.; Broadus, Matthew R.; Gould, Kathleen L.; Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232 ; Hanks, Steven K.; Polk, D. Brent

    2011-02-15

    The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway is a highly conserved signaling pathway that regulates diverse cellular processes including differentiation, proliferation, and survival. Kinase suppressor of Ras-1 (KSR1) binds each of the three ERK cascade components to facilitate pathway activation. Even though KSR1 contains a C-terminal kinase domain, evidence supporting the catalytic function of KSR1 remains controversial. In this study, we produced recombinant wild-type or kinase-inactive (D683A/D700A) KSR1 proteins in Escherichia coli to test the hypothesis that KSR1 is a functional protein kinase. Recombinant wild-type KSR1, but not recombinant kinase-inactive KSR1, underwent autophosphorylation on serine residue(s), phosphorylated myelin basic protein (MBP) as a generic substrate, and phosphorylated recombinant kinase-inactive MAPK/ERK kinase-1 (MEK1). Furthermore, FLAG immunoprecipitates from KSR1{sup -/-} colon epithelial cells stably expressing FLAG-tagged wild-type KSR1 (+KSR1), but not vector (+vector) or FLAG-tagged kinase-inactive KSR1 (+D683A/D700A), were able to phosphorylate kinase-inactive MEK1. Since TNF activates the ERK pathway in colon epithelial cells, we tested the biological effects of KSR1 in the survival response downstream of TNF. We found that +vector and +D683A/D700A cells underwent apoptosis when treated with TNF, whereas +KSR1 cells were resistant. However, +KSR1 cells were sensitized to TNF-induced cell loss in the absence of MEK kinase activity. These data provide clear evidence that KSR1 is a functional protein kinase, MEK1 is an in vitro substrate of KSR1, and the catalytic activities of both proteins are required for eliciting cell survival responses downstream of TNF.

  12. Down-regulation of gibberellic acid in poplar has negligible effects on host-plant suitability and insect pest response

    SciTech Connect (OSTI)

    Buhl, Christine; Strauss, Steven H.; Lindroth, Richard L.

    2015-01-06

    Abstract Endogenous levels and signaling of gibberellin plant hormones such as gibberellic acid (GA) have been genetically down-regulated to create semi-dwarf varieties of poplar. The potential benefits of semi-dwarf stature include reduced risk of wind damage, improved stress tolerance, and improved wood quality. Despite these benefits, modification of growth traits may have consequences for non-target traits that confer defense against insect herbivores. According to the growth-differentiation balance hypothesis, reductions in growth may shift allocation of carbon from growth to chemical resistance traits, thereby altering plant defense. To date, host-plant suitability and pest response have not been comprehensively evaluated in GA down-regulated plants. We quantified chemical resistance and nitrogen (an index of protein) in GA down-regulated and wild-type poplar (Populus alba P. tremula) genotypes. We also evaluated performance of both generalist (Lymantria dispar) and specialist (Chrysomela scripta) insect pests reared on these genotypes. Our evaluation of resistance traits in four GA down-regulated genotypes revealed increased phenolic glycosides in one modified genotype and reduced lignin in two modified genotypes relative to the non-transgenic wild type. Nitrogen levels did not vary significantly among the experimental genotypes. Generalists reared on the four GA down-regulated genotypes exhibited reduced performance on only one modified genotype relative to the wild type. Specialists, however, performed similarly across all genotypes. Results from this study indicate that although some non-target traits varied among GA down-regulated genotypes, the differences in poplar pest susceptibility were modest and highly genotype-specific.

  13. Viruses in laboratory-reared cactus moth, Cactoblastis cactorum (Lepidoptera: Pyralidae)

    SciTech Connect (OSTI)

    Marti, O.G.; Myers, R.E.; Carpenter, J.E.; Styer, E.L.

    2007-03-15

    The cactus moth, Cactoblastis cactorum (Lepidoptera: Pyralidae: Phycitinae), is a non-native species threatening a variety of native cacti, particularly endangered species of Opuntia (Zimmerman et al. 2001), on the coast of the Gulf of Mexico. Cactoblastis cactorum populations have expanded from Florida northward along the Atlantic coast as far as Charleston, SC, and westward along the Gulf of Mexico to Dauphin Island, south of Mobile, AL. It is feared that further movement to the west will allow C. cactorum to enter the US desert Southwest and Mexico, particularly the latter. Numerous cactus species, especially those of the genera Opuntia and Nopalea, are native to the U.S. and Mexico. Local economies based on agricultural and horticultural uses of cacti could be devastated by C. cactorum (Vigueras and Portillo 2001). A bi-national control program between the US and Mexico is being developed, utilizing the sterile insect technique (SIT). In the SIT program, newly emerged moths are irradiated with a {sup 60}Co source and released to mate with wild individuals. The radiation dose completely sterilizes the females and partially sterilizes the males. When irradiated males mate with wild females, the F1 progeny of these matings are sterile. In order for the SIT program to succeed, large numbers of moths must be reared from egg to adult on artificial diet in a quarantined rearing facility (Carpenter et al. 2001). Irradiated insects must then be released in large numbers at the leading edge of the invasive population and at times which coincide with the presence of wild individuals available for mating. Mortality from disease in the rearing colony disrupts the SIT program by reducing the numbers of insects available for release.

  14. Appliance Rebates: Frequently Asked Questions | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Andrea Spikes Former Communicator at DOE's National Renewable Energy Laboratory The appliance rebate program has been wildly successful in many states. So successful, in fact, that people have plenty of questions and aren't always finding the answers they need. Whether you're just learning about the appliance rebate program or have some nagging questions, I hope this information is helpful to you. For the most up-to-date information on rebates in your state, click on your state on Approved

  15. Impact of phenolic substrate and growth temperature on the arthrobacter chlorophenolicus proteome

    SciTech Connect (OSTI)

    Unell, Maria; Abraham, Paul E.; Shah, Manesh; Zhang, Bing; Ruckert, Christian; VerBerkmoes, Nathan C.; Jansson, Janet K.

    2009-02-15

    We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol or phenol at 5 C and 28 C; both for the wild type and a mutant strain with mass spectrometry based proteomics. A label free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.

  16. T-600: Security Advisory for Adobe Flash Player, Adobe Reader and Acrobat

    Broader source: Energy.gov [DOE]

    This vulnerability (CVE-2011-0611) could cause a crash and potentially allow an attacker to take control of the affected system. There are reports that this vulnerability is being exploited in the wild in targeted attacks via a Flash (.swf) file embedded in a Microsoft Word (.doc) file delivered as an email attachment, targeting the Windows platform. At this time, Adobe is not aware of any attacks via PDF targeting Adobe Reader and Acrobat. Adobe Reader X Protected Mode mitigations would prevent an exploit of this kind from executing.

  17. Beasley Lab | Savannah River Ecology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Recent Publications In Press Olson, Z.H., J.C. Beasley, and O.E. Rhodes, Jr. In Press. Carcass type affects local scavenger guilds more than habitat connectivity. PlosOne Keiter, D, J.J. Mayer, and J.C. Beasley. In Press. What’s in a “common” name? A call for consistent terminology for referring to non-native Sus scrofa. Wildlife Society Bulletin Webster, S.C., M.E. Byrne, S.L. Lance, C.L. Love, T. Hinton, D. Shamovich, and J.C. Beasley. In Press. Where the wild things are: Influence of

  18. Role of Nrf2 in preventing ethanol-induced oxidative stress and lipid accumulation

    SciTech Connect (OSTI)

    Wu, Kai Connie; Liu, Jie; Klaassen, Curtis D.

    2012-08-01

    Oxidative stress and lipid accumulation play important roles in alcohol-induced liver injury. Previous reports showed that, in livers of nuclear factor erythroid 2-related factor 2 (Nrf2)-activated mice, genes involved in antioxidant defense are induced, whereas genes involved in lipid biosynthesis are suppressed. To investigate the role of Nrf2 in ethanol-induced hepatic alterations, Nrf2-null mice, wild-type mice, kelch-like ECH-associated protein 1-knockdown (Keap1-KD) mice with enhanced Nrf2, and Keap1-hepatocyte knockout (Keap1-HKO) mice with maximum Nrf2 activation, were treated with ethanol (5 g/kg, po). Blood and liver samples were collected 6 h thereafter. Ethanol increased alanine aminotransferase and lactate dehydrogenase activities as well as thiobarbituric acid reactive substances in serum of Nrf2-null and wild-type mice, but not in Nrf2-enhanced mice. After ethanol administration, mitochondrial glutathione concentrations decreased markedly in Nrf2-null mice but not in Nrf2-enhanced mice. H{sub 2}DCFDA staining of primary hepatocytes isolated from the four genotypes of mice indicates that oxidative stress was higher in Nrf2-null cells, and lower in Nrf2-enhanced cells than in wild-type cells. Ethanol increased serum triglycerides and hepatic free fatty acids in Nrf2-null mice, and these increases were blunted in Nrf2-enhanced mice. In addition, the basal mRNA and nuclear protein levels of sterol regulatory element-binding protein 1(Srebp-1) were decreased with graded Nrf2 activation. Ethanol further induced Srebp-1 mRNA in Nrf2-null mice but not in Nrf2-enhanced mice. In conclusion, Nrf2 activation prevented alcohol-induced oxidative stress and accumulation of free fatty acids in liver by increasing genes involved in antioxidant defense and decreasing genes involved in lipogenesis. -- Highlights: ? Ethanol depleted mitochondrial GSH in Nrf2-null mice but not in Keap1-KD mice. ? Ethanol increased ROS in hepatocytes isolated from Nrf2-null and wild-type mice. ? Nrf2 blunted ethanol-induced increase of triglycerides and free fatty acids. ? The mRNA and nuclear protein of Srebp-1 were decreased with Nrf2 activation. ? The mRNA of Scd1 was increased in Nrf2-null mice after ethanol exposure.

  19. Tested by Fire - How two recent Wildfires affected Accelerator Operations at LANL

    SciTech Connect (OSTI)

    Spickermann, Thomas

    2012-08-01

    In a little more than a decade two large wild fires threatened Los Alamos and impacted accelerator operations at LANL. In 2000 the Cerro Grande Fire destroyed hundreds of homes, as well as structures and equipment at the DARHT facility. The DARHT accelerators were safe in a fire-proof building. In 2011 the Las Conchas Fire burned about 630 square kilometers (250 square miles) and came dangerously close to Los Alamos/LANL. LANSCE accelerator operations Lessons Learned during Las Conchas fire: (1) Develop a plan to efficiently shut down the accelerator on short notice; (2) Establish clear lines of communication in emergency situations; and (3) Plan recovery and keep squirrels out.

  20. User Data Forum Presentations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Presentations User Data Forum Presentations Sort by: Default | Name | Date (low-high) | Date (high-low) | Source | Category M01: Perspectives from DOE June 16, 2014 | Author(s): Laura Biven, Ph.D. Senior Science and Technology Advisor, DOE Office of the Deputy Director for Science Programs | Download File: M01-2014JuneNERSCBIVEN.pdf | pdf | 2.6 MB M02: The Future of Data and Scientific Workflow June 16, 2014 | Author(s): Michael Wilde, University of Chicago and Argonne National Laboratory |

  1. Pacific Lamprey Research and Restoration : Annual Report 1997.

    SciTech Connect (OSTI)

    Jackson, Aaron D.; Hatch, Douglas R.; Close, David A.

    1998-08-05

    The once abundant stocks of Pacific lamprey (Lampetra tridentata) above Bonneville Dam are currently depressed (Close et al. 1995). It is likely that many of the same factors that led to the decline of wild stocks of Columbia River Pacific salmon and steelhead have impacted Pacific lamprey populations as well. The Pacific Lamprey Research and Restoration Project, funded by Bonneville Power Administration, is a cooperative effort between the Confederated Tribes of the Umatilla Indian Reservation, the Columbia River Inter-Tribal Fish Commission, and Oregon State University with the goal to increase Pacific lamprey stocks above Bonneville Dam.

  2. Analyzing Surface Solar Flux Data in Oregon for Changes Due to Aerosols Laura D. Riihimaki1, Frank E. Vignola1, Charles N. Long2, James A. Coakley Jr.3 1 University of Oregon Solar Radiation Monitoring Lab 2 Pacific Northwest National Laboratory 3 Oregon State University, College of Oceanic and Atmospheric Sciences

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Significant Decadal Brightening of Downwelling Shortwave in the Continental US C. N. Long, E. G. Dutton, J. A. Augustine, W. Wiscombe, M. Wild, S. A. McFarlane, and C. J. Flynn 5. Little relationship between AOD and clear-sky SW anomalies, but significant relationship between sky cover and all-sky SW anomalies. CONCLUSIONS: Downwelling SW has increased by 8 Wm -2 /decade since 1996 over the US. This is 3 times the corresponding wide-spread 2-3 Wm -2 LW increase reported from GEBA records

  3. Effect of the L499M mutation of the ascomycetous Botrytis aclada laccase on redox potential and catalytic properties

    SciTech Connect (OSTI)

    Osipov, Evgeny [A. N. Bach Institute of Biochemistry, Leninsky Prospect 33/2, Moscow 119071 (Russian Federation); Polyakov, Konstantin [A. N. Bach Institute of Biochemistry, Leninsky Prospect 33/2, Moscow 119071 (Russian Federation); Engelhardt Institute of Molecular Biology, Vavilova Str. 32, Moscow 119991 (Russian Federation); Kittl, Roman [BOKU University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Wien (Austria); Shleev, Sergey [RSC Kurchatov Institute, Acad. Kurchatov Sq. 1, Moscow 123182 (Russian Federation); Malm University, 205 06 Malm (Sweden); Dorovatovsky, Pavel [RSC Kurchatov Institute, Acad. Kurchatov Sq. 1, Moscow 123182 (Russian Federation); Tikhonova, Tamara, E-mail: ttikhonova@inbi.ras.ru [A. N. Bach Institute of Biochemistry, Leninsky Prospect 33/2, Moscow 119071 (Russian Federation); Hann, Stephan; Ludwig, Roland [BOKU University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Wien (Austria); Popov, Vladimir [A. N. Bach Institute of Biochemistry, Leninsky Prospect 33/2, Moscow 119071 (Russian Federation); RSC Kurchatov Institute, Acad. Kurchatov Sq. 1, Moscow 123182 (Russian Federation)

    2014-11-01

    The structures of the ascomycetous B. aclada laccase and its L499M T1-site mutant have been solved at 1.7 resolution. The mutant enzyme shows a 140 mV lower redox potential of the type 1 copper and altered kinetic behaviour. The wild type and the mutant have very similar structures, which makes it possible to relate the changes in the redox potential to the L499M mutation Laccases are members of a large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of dioxygen to water. These enzymes contain four Cu atoms per molecule organized into three sites: T1, T2 and T3. In all laccases, the T1 copper ion is coordinated by two histidines and one cysteine in the equatorial plane and is covered by the side chains of hydrophobic residues in the axial positions. The redox potential of the T1 copper ion influences the enzymatic reaction and is determined by the nature of the axial ligands and the structure of the second coordination sphere. In this work, the laccase from the ascomycete Botrytis aclada was studied, which contains conserved Ile491 and nonconserved Leu499 residues in the axial positions. The three-dimensional structures of the wild-type enzyme and the L499M mutant were determined by X-ray crystallography at 1.7 resolution. Crystals suitable for X-ray analysis could only be grown after deglycosylation. Both structures did not contain the T2 copper ion. The catalytic properties of the enzyme were characterized and the redox potentials of both enzyme forms were determined: E{sub 0} = 720 and 580 mV for the wild-type enzyme and the mutant, respectively. Since the structures of the wild-type and mutant forms are very similar, the change in the redox potential can be related to the L499M mutation in the T1 site of the enzyme.

  4. Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by

    Office of Scientific and Technical Information (OSTI)

    phosphorylating the CDK-inhibitor Rum1 (Journal Article) | SciTech Connect Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by phosphorylating the CDK-inhibitor Rum1 Citation Details In-Document Search Title: Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by phosphorylating the CDK-inhibitor Rum1 Highlights: ► Deletion of lkh1{sup +} made cells pass the G1/S phase faster than the wild type. ► Lkh1 can interact with a cyclin-dependent kinase inhibitor (CKI) Rum1. ►

  5. Environmental profile of Paraguay

    SciTech Connect (OSTI)

    Not Available

    1985-12-01

    The social, cultural, physical, and economic dimensions of Paraguay's environment are analyzed to identify main environmental features and problems and to recommend specific actions. The environmental profile presents an overview of Paraguay's ethno-historic and anthropological background, present-day society, and the impact of pollution. Descriptions are presented of: the legal and institutional aspects of environmental policy; the structure and performance of the economy, with focus on the primary and energy sectors; physical resources (climate, geological, mineral, soil, and water resources); and biological resources (vegetation, wild animal life, protected areas, and fish resources).

  6. Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme

    DOE Patents [OSTI]

    Koch, Daniel J.; Arnold, Frances H.

    2013-01-29

    Cytochrome P450 CYP153A6 from Myobacterium sp. strain HXN1500 was engineered using in-vivo directed evolution to hydroxylate small-chain alkanes regioselectively. Mutant CYP153A6-BMO1 selectively hydroxylates butane and pentane at the terminal carbon to form 1-butanol and 1-pentanol, respectively, at rates greater than wild-type CYP153A6 enzymes. This biocatalyst is highly active for small-chain alkane substrates and the regioselectivity is retained in whole-cell biotransformations.

  7. WIPP Receives New Emergency Response Vehicle

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    February 19, 2015 WIPP Receives New Emergency Response Vehicle WIPP recently placed a new emergency response vehicle into service. The new fire engine "Engine 24" will enhance emergency response capabilities at the WIPP facility. The new four-wheel drive vehicle was designed to respond to both structure and wild land fires. Capable of carrying 1,500 gallons of water on board, the engine has the ability to spray water or foam through a remote-controlled turret located on the front

  8. Plants & Animals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plants & Animals Plants & Animals Plant and animal monitoring is performed to determine whether Laboratory operations are impacting human health via the food chain. February 2, 2015 A rabbit on LANL land. A rabbit on LANL land. Contact Environmental Communication & Public Involvement P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505) 667-0216 Email We sample many plants and animals, including wild and domestic crops, game animals, fish, and food products from animals, as well as other

  9. Flight Path 60R - GEANIE Collaborators

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Target 4 Flight Path 60R Collaborators LANL: M. Devlin, N. Fotiades, R. O Nelson, A. Couture, M.S. Boswell, D.R. Mayo, F. Merrill, S. Mosby, J. M. O'Donnell, R. Rundberg, S, Stange, S. Vogel, C. Wilde CEA DAM/DIF: G. Belier, T. Granier, T. Ethvignot LLNL: C. Y. Wu, A. Chyzh, J. Becker, E, Kwan, J. Escher, J, Burke, N. Scielzo, R. Hatarik U.S. Army Research Laboratory: J. Carroll, M. Litz Indiana University: T. Johnson, J. Albert, L. Kaufman, S. Dougherty Rutgers University: J. Cizewski, B.

  10. Compositions of Stardust Impact Tracks and Terminal Particles in Aerogel by

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hard X-ray Microprobe at SSRL Compositions of Stardust Impact Tracks and Terminal Particles in Aerogel by Hard X-ray Microprobe at SSRL Until 1974, Comet 81P/Wild 2 orbited beyond Jupiter, but a gravitational kick from that planet altered its orbit transforming it into a short-period comet in the inner solar system. This allowed NASA's solar-powered Stardust spacecraft to intercept the comet's tail within the orbit of Mars. The fly-by was completed at a relative speed of 6.1 km/s passing

  11. DOE - NNSA/NFO -- Plants and Animals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plants & Animals NNSA/NFO Language Options U.S. DOE/NNSA - Nevada Field Office Click to subscribe to NNSS News Plants and Animals Wild Horses at the NNSS Because of its physical location, the NNSS is host to thousands of organisms that live either in the Mojave Desert, the Great Basin Desert, or in transitional habitat between these two deserts. There are 754 different species of plants, about 1200 invertebrates, 34 reptiles, 239 birds, and 60 mammals which are known to occur on the NNSS.

  12. John Roysters TAT success story

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    John Royster's TAT success story Our next story from Training and Technology history comes from John Royster, who writes: "I really enjoyed your story on Mayme Crowell. It brought back a lot of memories for me. There were some truly remarkable people on staff at the TAT program. "I was a 1973/74 TAT graduate. I feel, and I always have, that TAT guided me down a path that probably saved my life. I was 16 years old at the time. My father had passed away when I was 12. I was pretty wild.

  13. LA-13859-MS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    13859-MS Issued: September 2001 Nevada Test Site Radionuclide Inventory, 1951-1992 Scott M. Bowen David L. Finnegan Joseph L. Thompson Charles M. Miller (Deceased) Phyllis L. Baca Loretta F. Olivas Carmen G. Geoffrion David K. Smith* Wataru Goishi* Bradley K. Esser* Jesse W. Meadows* Neil Namboodiri* John F. Wild* *Lawrence Livermore National Laboratory, 7000 East Ave., Livermore, CA 94550-9234 Los Alamos N A T I O N A L L A B O R A T O R Y Los Alamos, New Mexico 87545 Nevada Test Site

  14. Characterization of Sugar Insensitive (sis) Mutants of Arabidopsis

    SciTech Connect (OSTI)

    Gibson, Susan I.

    2009-06-08

    Despite the fact that soluble sugar levels have been postulated to play an important role in the control of a wide variety of plant metabolic and developmental pathways, the mechanisms by which plants respond to soluble sugar levels remain poorly understood. Plant responses to soluble sugar levels are also important in bioenergy production, as plant sugar responses are believed to help regulate both carbon fixation and carbon partitioning. For example, accumulation of soluble sugars, such as sucrose and glucose, in source tissues leads to feedback inhibition of photosynthesis, thereby decreasing rates of carbon fixation. Soluble sugar levels can also affect sink strengths, affecting the rates of accumulation of carbon-based compounds into both particular molecular forms (e.g. carbohydrates versus lipids versus proteins) and particular plant organs and tissues. Mutants of Arabidopsis that are defective in the ability to respond to soluble sugar levels were isolated and used as tools to identify some of the factors involved in plant sugar response. These sugar insensitive (sis) mutants were isolated by screening mutagenized seeds for those that were able to germinate and develop relatively normal shoot systems on media containing 0.3 M glucose or 0.3 M sucrose. At these sugar concentrations, wild-type Arabidopsis germinate and produce substantial root systems, but show little to no shoot development. Twenty-eight sis mutants were isolated during the course of four independent mutant screens. Based on a preliminary characterization of all of these mutants, sis3 and sis6 were chosen for further study. Both of these mutations appear to lie in previously uncharacterized loci. Unlike many other sugar-response mutants, sis3 mutants exhibit a wild-type or near wild-type response in all phytohormone-response assays conducted to date. The sis6-1 mutation is unusual in that it appears to be due to overexpression of a gene, rather than representing a loss of function mutation. Characterization of mutant and wild-type plants has revealed that sugars inhibit breakdown of seed storage lipids. In addition, high concentrations of exogenous sugars largely eliminate the development of mature chloroplasts by developing seedlings. Affymetrix GeneChip experiments have revealed that expression of many plant genes is partially regulated by sugar levels, with approximately two percent of genes exhibiting alterations in steady-state mRNA levels in response to changing sugar concentrations. Ultimately, a better understanding of plant sugar responses may allow improvements in rates of carbon fixation and manipulation of carbon partitioning. These improvements will be needed to help make production of energy from biomass more economically attractive.

  15. Comparative Survival Study (CSS) of PIT-Tagged Spring/Summer Chinook and Summer Steelhead : 2008 Annual Report.

    SciTech Connect (OSTI)

    Comparative Survival Study Oversight Committee and Fish Passage Center

    2008-12-02

    The Comparative Survival Study (CSS; BPA Project 199602000) began in 1996 with the objective of establishing a long term dataset of the survival rate of annual generations of salmon from their outmigration as smolts to their return to freshwater as adults to spawn (smolt-to-adult return rate; SAR). The study was implemented with the express need to address the question whether collecting juvenile fish at dams and transporting them downstream in barges and trucks and releasing them downstream of Bonneville Dam was compensating for the effect of the Federal Columbia River Power System (FCRPS) on survival of Snake Basin spring/summer Chinook salmon migrating through the hydrosystem. The Completion of this annual report for the CSS signifies the 12th outmigration year of hatchery spring/summer Chinook salmon marked with Passive Integrated Transponder (PIT) tags as part of the CSS and the 9th complete brood year return as adults of those PIT-tagged fish (report covers adult returns from 1997-2006 hatchery Chinook juvenile migrations). In addition, the CSS has provided PIT-tags to on-going tagging operations for wild Chinook since 2002 (report covers adult returns from 1994-2006 wild Chinook juvenile migrations). The CSS tags wild steelhead on the lower Clearwater River and utilized wild and hatchery steelhead from other tagging operations in evaluations of transportation (report covers adult returns from 1997-2005 wild and hatchery steelhead migrations). The primary purpose of this report is to update the time series of smolt-to-adult survival rate data and related parameters with additional years of data since the completion of the CSS 10-yr retrospective analysis report (Schaller et al 2007). The 10-yr report provided a synthesis of the results from this ongoing study, the analytical approaches employed, and the evolving improvements incorporated into the study as reported in CSS annual progress reports. This current report specifically addresses the constructive comments of the most recent regional technical review conducted by the Independent Scientific Advisory Board and Independent Scientific Review Panel (ISAB and ISRP 2007). This report completes the 3-salt returns from migration years 2004 for wild and hatchery Chinook and steelhead (all returns are to Lower Granite Dam). For wild and hatchery Chinook, this report also provides 3-salt returns from migration year 2005 and 2-salt returns from migration year 2006 through a cutoff date of August 13, 2008. For wild and hatchery steelhead, it provides completed 2-salt returns for wild and hatchery steelhead that outmigrated in 2005 (any 3-salt returns of PIT-tagged steelhead are few, but will occur after July 1, 2008). All of the Chinook salmon evaluated in the CSS study exhibit a stream-type life history. All study fish used in this report were uniquely identifiable based on a PIT-tag implanted in the body cavity during (or before) the smolt life stage and retained through their return as adults. These tagged fish can then be detected as juveniles and adults at several locations of the Snake and Columbia rivers. Reductions in the number of individuals detected as the tagged fish grow older provide estimates of survival. This allows comparisons of survival over different life stages between fish with different experiences in the hydrosystem (e.g. transportation vs. in-river migrants and migration through various numbers of dams) as illustrated in Figure 1.1. The CSS is a long term study within the Northwest Power and Conservation Council's Columbia Basin Fish and Wildlife Program (NPCC FWP) and is funded by Bonneville Power Administration (BPA). Study design and analyses are conducted through a CSS Oversight Committee with representation from Columbia River Inter-Tribal Fish Commission (CRITFC), Idaho Department of Fish and Game (IDFG), Oregon Department of Fish and Wildlife (ODFW), U.S. Fish and Wildlife Service (USFWS), and Washington Department of Fish and Wildlife (WDFW). The Fish Passage Center (FPC) coordinates the PIT-tagging efforts, data management and preparation

  16. Microsoft Word - FeMoco.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fig. 1. Fourier transforms calculated over the k-range 2-16 Å -1 of EXAFS data (dotted) and fits (solid) from (left) MoFe protein- bound FeMoco and (right) the NifEN-bound FeMoco precursor. The EXAFS data were obtained, respectively, by subtraction of ΔnifB MoFe protein EXAFS from wild-type MoFe protein EXAFS and ΔnifB NifEN EXAFS from NifEN EXAFS. The insets show the structure of FeMoco (left) and a structural model consistent with the XAS analysis of the NifEN-bound FeMoco precursor

  17. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Switch to Detail View for this search SciTech Connect Search Results Page 1 of 1 Search for: All records Creators/Authors contains: "Wild, Stefan" × Sort by Relevance Sort by Date (newest first) Sort by Date (oldest first) Sort by Relevance « Prev Next » Everything4 Electronic Full Text3 Citations1 Multimedia0 Datasets0 Software0 Filter Results Filter by Subject mathematics and computing (2) applied mathematics (1) big data (1) computational science (1) computer science (1)

  18. Decreasing transmembrane segment length greatly decreases perfringolysin O pore size

    SciTech Connect (OSTI)

    Lin, Qingqing; Li, Huilin; Wang, Tong; London, Erwin

    2015-04-08

    Perfringolysin O (PFO) is a transmembrane (TM) β-barrel protein that inserts into mammalian cell membranes. Once inserted into membranes, PFO assembles into pore-forming oligomers containing 30–50 PFO monomers. These form a pore of up to 300 Å, far exceeding the size of most other proteinaceous pores. In this study, we found that altering PFO TM segment length can alter the size of PFO pores. A PFO mutant with lengthened TM segments oligomerized to a similar extent as wild-type PFO, and exhibited pore-forming activity and a pore size very similar to wild-type PFO as measured by electron microscopy and a leakage assay. In contrast, PFO with shortened TM segments exhibited a large reduction in pore-forming activity and pore size. This suggests that the interaction between TM segments can greatly affect the size of pores formed by TM β-barrel proteins. PFO may be a promising candidate for engineering pore size for various applications.

  19. Superoxide reduction by a superoxide reductase lacking the highly conserved lysine residue

    SciTech Connect (OSTI)

    Teixeira, Miguel; Cabelli, Diane; Pinto, Ana F.; Romao, Celia V.; Pinto, Liliana C.; Huber, Harald; Saraiva, Ligia M.; Todorovic, Smilja

    2014-12-05

    Superoxide reductases (SORs) are the most recently identified superoxide detoxification systems, being found in microorganisms from the three domains of life. These enzymes are characterized by a catalytic mononuclear iron site, with one cysteine and four histidine ligands of the ferrous active form. A lysine residue in the EKHVP motif, located close to the active site, has been considered to be essential for the enzyme function, by contributing to the positive surface patch that attracts the superoxide anion and by controlling the chemistry of the catalytic mechanism through a hydrogen bond network. However, we show here that this residue is substituted by non-equivalent amino acids in several putative SORs from Archaea and unicellular Eukarya. In this work, we focus on mechanistic and spectroscopic studies of one of these less common enzymes, the SOR from the hyperthermophilic crenarchaeon Ignicoccus hospitalis. We employ pulse radiolysis fast kinetics and spectroscopic approaches to study the wild-type enzyme (?E??T??HVP?), and two mutants, T24K and E23A, the later mimicking enzymes lacking both the lysine and glutamate (a ferric ion ligand) of the motif. The efficiency of the wild type protein and mutants in reducing superoxide is comparable to other SORs, revealing the robustness of these enzymes to single mutations.

  20. Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions

    SciTech Connect (OSTI)

    Gonzalez, Silvia A.; Paladino, Monica G.; Affranchino, Jose L.

    2012-06-20

    The feline immunodeficiency virus (FIV) envelope glycoprotein (Env) possesses a short cytoplasmic domain of 53 amino acids containing four highly conserved cysteines at Env positions 804, 811, 815 and 848. Since palmitoylation of transmembrane proteins occurs at or near the membrane anchor, we investigated whether cysteines 804, 811 and 815 are acylated and analyzed the relevance of these residues for Env functions. Replacement of cysteines 804, 811 and 815 individually or in combination by serine residues resulted in Env glycoproteins that were efficiently expressed and processed. However, mutations C804S and C811S reduced Env fusogenicity by 93% and 84%, respectively, compared with wild-type Env. By contrast, mutant C815S exhibited a fusogenic capacity representing 50% of the wild-type value. Remarkably, the double mutation C804S/C811S abrogated both Env fusion activity and Env incorporation into virions. Finally, by means of Click chemistry assays we demonstrated that the four FIV Env cytoplasmic cysteines are palmitoylated.

  1. Structural Basis for a Switch in Receptor Binding Specificity of Two H5N1 Hemagglutinin Mutants

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhu, Xueyong; Viswanathan, Karthik; Raman, Rahul; Yu, Wenli; Sasisekharan, Ram; Wilson, Ian A.

    2015-11-01

    Avian H5N1 influenza viruses continue to spread in wild birds and domestic poultry with sporadic infection in humans. Receptor binding specificity changes are a prerequisite for H5N1 viruses and other zoonotic viruses to be transmitted among humans. Previous reported hemagglutinin (HA) mutants from ferret-transmissible H5N1 viruses of A/Viet Nam/1203/04 and A/Indonesia/5/05 showed slightly increased, but still very weak, binding to human receptors. From mutagenesis and glycan array studies, we previously identified two H5N1 HA mutants that could more effectively switch receptor specificity to human-like α2-6 linked sialosides with avidity comparable to wild-type H5 HA binding to avian-like α2-3 linked sialosides.more »Here, crystal structures of these two H5 HA mutants free and in complex with human and avian glycan receptor analogues reveal the structural basis for their preferential binding to human receptors. These findings suggest continuous surveillance should be maintained to monitor and assess human-to-human transmission potential of H5N1 viruses.« less

  2. Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellum strain

    SciTech Connect (OSTI)

    Yee, Kelsey L; Rodriguez Jr, Miguel; Thompson, Olivia A; Fu, Chunxiang; Wang, Zeng-Yu; Davison, Brian H; Mielenz, Jonathan R

    2014-01-01

    Background: Switchgrass is an abundant and dedicated bioenergy feedstock however its inherent recalcitrance is one of the economic hurdles for producing biofuels. The down-regulation of the caffeic acid O-methyl transferase (COMT) gene in the lignin pathway of switchgrass reduced lignin content and S/G ratio, and the transgenic lines showed improved fermentation yield with S. cerevisiae and C. thermocellum (ATCC 27405) in comparison to the wild-type switchgrass. Results: Here we examine the fermentation potential of the COMT transgenic switchgrass and its wild-type line, with an engineered and evolved Clostridium thermocellum (M1570) strain. The fermentation of the transgenic switchgrass had superior conversion relative to the control line with an increase of 20% and ethanol was the primary metabolite accounting for 90% of the total metabolites measured by HPLC. Conclusions: The down-regulation of the COMT gene in switchgrass reduced recalcitrance and improved microbial bioconversion yield. Moreover, these results showed ethanol as the main fermentation metabolite produced by an engineered and evolved C. thermocellum strain grown on a transgenic switchgrass.

  3. Direct Conversion of Plant Biomass to Ethanol by Engineered Caldicellulosiruptor bescii

    SciTech Connect (OSTI)

    Chung, Daehwan; Cha, Minseok; Guss, Adam M; Westpheling, Janet

    2014-01-01

    Ethanol is the most widely used renewable transportation biofuel in the United States, with the production of 13.3 billion gallons in 2012 [John UM (2013) Contribution of the Ethanol Industry to the Economy of the United States]. Despite considerable effort to produce fuels from lignocellulosic biomass, chemical pretreatment and the addition of saccharolytic enzymes before microbial bioconversion remain economic barriers to industrial deployment [Lynd LR, et al. (2008) Nat Biotechnol 26(2):169-172]. We began with the thermophilic, anaerobic, cellulolytic bacterium Caldicellulosiruptor bescii, which efficiently uses unpretreated biomass, and engineered it to produce ethanol. Here we report the direct conversion of switchgrass, a nonfood, renewable feedstock, to ethanol without conventional pretreatment of the biomass. This process was accomplished by deletion of lactate dehydrogenase and heterologous expression of a Clostridium thermocellum bifunctional acetaldehyde/alcohol dehydrogenase. Whereas wild-type C. bescii lacks the ability to make ethanol, 70% of the fermentation products in the engineered strain were ethanol [12.8 mM ethanol directly from 2% (wt/vol) switchgrass, a real-world substrate] with decreased production of acetate by 38% compared with wild-type. Direct conversion of biomass to ethanol represents a new paradigm for consolidated bioprocessing, offering the potential for carbon neutral, cost-effective, sustainable fuel production.

  4. Superoxide reduction by a superoxide reductase lacking the highly conserved lysine residue

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Teixeira, Miguel; Cabelli, Diane; Pinto, Ana F.; Romao, Celia V.; Pinto, Liliana C.; Huber, Harald; Saraiva, Ligia M.; Todorovic, Smilja

    2014-12-05

    Superoxide reductases (SORs) are the most recently identified superoxide detoxification systems, being found in microorganisms from the three domains of life. These enzymes are characterized by a catalytic mononuclear iron site, with one cysteine and four histidine ligands of the ferrous active form. A lysine residue in the –EKHVP– motif, located close to the active site, has been considered to be essential for the enzyme function, by contributing to the positive surface patch that attracts the superoxide anion and by controlling the chemistry of the catalytic mechanism through a hydrogen bond network. However, we show here that this residue ismore » substituted by non-equivalent amino acids in several putative SORs from Archaea and unicellular Eukarya. In this work, we focus on mechanistic and spectroscopic studies of one of these less common enzymes, the SOR from the hyperthermophilic crenarchaeon Ignicoccus hospitalis. We employ pulse radiolysis fast kinetics and spectroscopic approaches to study the wild-type enzyme (₋E₂₃T₂₄HVP₋), and two mutants, T24K and E23A, the later mimicking enzymes lacking both the lysine and glutamate (a ferric ion ligand) of the motif. The efficiency of the wild type protein and mutants in reducing superoxide is comparable to other SORs, revealing the robustness of these enzymes to single mutations.« less

  5. Yellow fluorescent protein phiYFPv (Phialidium): structure and structure-based mutagenesis

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z., E-mail: vzpletnev@gmail.com; Souslova, Ekaterina; Chudakov, Dmitry M. [Russian Academy of Sciences, Moscow (Russian Federation); Lukyanov, Sergey [Russian Academy of Sciences, Moscow (Russian Federation); Nizhny Novgorod State Medical Academy, Nizhny Novgorod (Russian Federation); Martynov, Vladimir I.; Arhipova, Svetlena; Artemyev, Igor [Russian Academy of Sciences, Moscow (Russian Federation); Wlodawer, Alexander [National Cancer Institute, Frederick, MD 21702 (United States); Dauter, Zbigniew [National Cancer Institute, Argonne, IL 60439 (United States); Pletnev, Sergei [National Cancer Institute, Argonne, IL 60439 (United States); SAIC-Frederick, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Russian Academy of Sciences, Moscow (Russian Federation)

    2013-06-01

    The yellow fluorescent protein phiYFPv with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The yellow fluorescent protein phiYFPv (?{sub em}{sup max} ? 537 nm) with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The latter fluorescent protein is one of only two known cases of naturally occurring proteins that exhibit emission spectra in the yelloworange range (535555 nm). Here, the crystal structure of phiYFPv has been determined at 2.05 resolution. The yellow chromophore formed from the sequence triad Thr65-Tyr66-Gly67 adopts the bicyclic structure typical of fluorophores emitting in the green spectral range. It was demonstrated that perfect antiparallel ?-stacking of chromophore Tyr66 and the proximal Tyr203, as well as Val205, facing the chromophore phenolic ring are chiefly responsible for the observed yellow emission of phiYFPv at 537 nm. Structure-based site-directed mutagenesis has been used to identify the key functional residues in the chromophore environment. The obtained results have been utilized to improve the properties of phiYFPv and its homologous monomeric biomarker tagYFP.

  6. The HPr Proteins from the Thermophile Bacillus stearothermophilus Can Form Domain-swapped Dimers

    SciTech Connect (OSTI)

    Sridharan, Sudharsan; Razvi, Abbas; Scholtz, J. Martin; Sacchettini, James C. (TAM)

    2010-07-20

    The study of proteins from extremophilic organisms continues to generate interest in the field of protein folding because paradigms explaining the enhanced stability of these proteins still elude us and such studies have the potential to further our knowledge of the forces stabilizing proteins. We have undertaken such a study with our model protein HPr from a mesophile, Bacillus subtilis, and a thermophile, Bacillus stearothermophilus. We report here the high-resolution structures of the wild-type HPr protein from the thermophile and a variant, F29W. The variant proved to crystallize in two forms: a monomeric form with a structure very similar to the wild-type protein as well as a domain-swapped dimer. Interestingly, the structure of the domain-swapped dimer for HPr is very different from that observed for a homologous protein, Crh, from B. subtilis. The existence of a domain-swapped dimer has implications for amyloid formation and is consistent with recent results showing that the HPr proteins can form amyloid fibrils. We also characterized the conformational stability of the thermophilic HPr proteins using thermal and solvent denaturation methods and have used the high-resolution structures in an attempt to explain the differences in stability between the different HPr proteins. Finally, we present a detailed analysis of the solution properties of the HPr proteins using a variety of biochemical and biophysical methods.

  7. Calmodulin Methionine Residues are Targets For One-Electron Oxidation by Hydroxyl Radicals: Formation of S therefore N three-electron bonded Radical Complexes

    SciTech Connect (OSTI)

    Nauser, Thomas; Jacoby, Michael E.; Koppenol, Willem H.; Squier, Thomas C.; Schoneich, Christian

    2005-02-01

    The one-electron (1e) oxidation of organic sulfides and methionine (Met) constitutes an important reaction mechanism in vivo.1,2 Evidence for a Cu(II)-catalyzed oxidation of Met35 in the Alzheimer's disease -amyloid peptide was obtained,3 and, based on theoretical studies, Met radical cations were proposed as intermediates.4 In the structure of -amyloid peptide, the formation of Met radical cations appears to be facilitated by a preexisting close sulfur-oxygen (S-O) interaction between the Met35 sulfur and the carbonyl oxygen of the peptide bond C-terminal to Ile31.5 Substitution of Ile31 with Pro31 abolishes this S-O interaction,5 significantly reducing the ability of -amyloid to reduce Cu(II), and converts the neurotoxic wild-type -amyloid into a non-toxic peptide.6 The preexisting S-O bond characterized for wild-type -amyloid suggests that electron transfer from Met35 to Cu(II) is supported through stabilization of the Met radical cation by the electron-rich carbonyl oxygen, generating an SO-bonded7 sulfide radical cation (Scheme 1, reaction 1).5

  8. Global warming accelerates drought-induced forest death

    ScienceCinema (OSTI)

    McDowell, Nathan; Pockman, William

    2014-06-02

    Many southwestern forests in the United States will disappear or be heavily altered by 2050, according to a series of joint Los Alamos National Laboratory-University of New Mexico studies. Nathan McDowell, a Los Alamos plant physiologist, and William Pockman, a UNM biology professor, explain that their research, and more from scientists around the world, is forecasting that by 2100 most conifer forests should be heavily disturbed, if not gone, as air temperatures rise in combination with drought. "Everybody knows trees die when there's a drought, if there's bark beetles or fire, yet nobody in the world can predict it with much accuracy." McDowell said. "What's really changed is that the temperature is going up," thus the researchers are imposing artificial drought conditions on segments of wild forest in the Southwest and pushing forests to their limit to discover the exact processes of mortality and survival. The study is centered on drought experiments in woodlands at both Los Alamos and the Sevilleta National Wildlife Refuge in central New Mexico. Both sites are testing hypotheses about how forests die on mature, wild trees, rather than seedlings in a greenhouse, through the ecosystem-scale removal of 50 percent of yearly precipitation through large water-diversion trough systems.

  9. Raptor Use of the Rio Grande Gorge

    SciTech Connect (OSTI)

    Ponton, David A.

    2015-03-20

    The Rio Grande Gorge is a 115 km long river canyon located in Southern Colorado (15 km) and Northern New Mexico (100 km). The majority of the canyon is under the administration of the Bureau of Land Management {BLM), and 77 km of the canyon south of the Colorado/New Mexico border are designated Wild River under the National Wild and Scenic Rivers Act of 1968. Visits I have made to the Rio Grande Gorge over the past 15 .years disclosed some raptor utilization. As the Snake River Birds of Prey Natural Area gained publicity, its similarity to the Rio Grande Gorge became obvious, and I was intrigued by the possibility of a high raptor nesting density in the Gorge. A survey in 1979 of 20 km of the northern end of the canyon revealed a moderately high density of red-tailed hawks and prairie falcons. With the encouragement of that partial survey, and a need to assess the impact of river-running on nesting birds of prey, I made a more comprehensive survey in 1980. The results of my surveys, along with those of a 1978 helicopter survey by the BLM, are presented in this report, as well as general characterization of the area, winter use by raptors, and an assessment of factors influencing the raptor population.

  10. Global warming accelerates drought-induced forest death

    SciTech Connect (OSTI)

    McDowell, Nathan; Pockman, William

    2013-07-09

    Many southwestern forests in the United States will disappear or be heavily altered by 2050, according to a series of joint Los Alamos National Laboratory-University of New Mexico studies. Nathan McDowell, a Los Alamos plant physiologist, and William Pockman, a UNM biology professor, explain that their research, and more from scientists around the world, is forecasting that by 2100 most conifer forests should be heavily disturbed, if not gone, as air temperatures rise in combination with drought. "Everybody knows trees die when there's a drought, if there's bark beetles or fire, yet nobody in the world can predict it with much accuracy." McDowell said. "What's really changed is that the temperature is going up," thus the researchers are imposing artificial drought conditions on segments of wild forest in the Southwest and pushing forests to their limit to discover the exact processes of mortality and survival. The study is centered on drought experiments in woodlands at both Los Alamos and the Sevilleta National Wildlife Refuge in central New Mexico. Both sites are testing hypotheses about how forests die on mature, wild trees, rather than seedlings in a greenhouse, through the ecosystem-scale removal of 50 percent of yearly precipitation through large water-diversion trough systems.

  11. Decreasing transmembrane segment length greatly decreases perfringolysin O pore size

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lin, Qingqing; Li, Huilin; Wang, Tong; London, Erwin

    2015-04-08

    Perfringolysin O (PFO) is a transmembrane (TM) β-barrel protein that inserts into mammalian cell membranes. Once inserted into membranes, PFO assembles into pore-forming oligomers containing 30–50 PFO monomers. These form a pore of up to 300 Å, far exceeding the size of most other proteinaceous pores. In this study, we found that altering PFO TM segment length can alter the size of PFO pores. A PFO mutant with lengthened TM segments oligomerized to a similar extent as wild-type PFO, and exhibited pore-forming activity and a pore size very similar to wild-type PFO as measured by electron microscopy and a leakagemore » assay. In contrast, PFO with shortened TM segments exhibited a large reduction in pore-forming activity and pore size. This suggests that the interaction between TM segments can greatly affect the size of pores formed by TM β-barrel proteins. PFO may be a promising candidate for engineering pore size for various applications.« less

  12. John Day River Sub-Basin Fish Habitat Enhancement Project; 2008 Annual Report

    SciTech Connect (OSTI)

    Powell, Russ M.; Alley, Pamela D.; Goin Jr, Lonnie

    2009-07-15

    Work undertaken in 2008 included: (1) Seven new fence projects were completed thereby protecting approximately 10.97 miles of streams with 16.34 miles of riparian fence; (2) Renewal of one expired lease was completed thereby continuing to protect 0.75 miles of stream with 1.0 mile of riparian fence. (3) Maintenance of all active project fences (106.54 miles), watergaps (78), spring developments (33) were checked and repairs performed; (3) Planted 1000 willow/red osier on Fox Creek/Henslee property; (4) Planted 2000 willows/red osier on Middle Fork John Day River/Coleman property; (5) Planted 1000 willow/red osier cuttings on Fox Creek/Johns property; (6) Since the initiation of the Fish Habitat Project in 1984 we have 126.86 miles of stream protected using 211.72 miles of fence protecting 5658 acres. The purpose of the John Day Fish Habitat Enhancement Program is to enhance production of indigenous wild stocks of spring Chinook and summer steelhead within the sub basin through habitat protection, enhancement and fish passage improvement. The John Day River system supports the largest remaining wild runs of spring chinook salmon and summer steelhead in Northeast Oregon.

  13. The Magnificent Journey.

    SciTech Connect (OSTI)

    United States. Bonneville Power Administration.

    1995-01-01

    The annual run of Northwest salmon--from the vast Pacific Ocean to the mountain streams where their lives began--is one of Nature`s most awe-inspiring events. Now that modern science has discovered some of the salmon`s secrets, their journey seems even more miraculous. So unlikely is the survival of a single returning salmon that Nature compensates heavily. Of the other 3,000 to 7,000 eggs in a nest, only one spawning pair, on average, will make it back. Too much or too little water at hatching can wipe out great swarms of young fish life. Bigger fish, bears, seals--all take their share of salmon. Nature allows for these natural events. But Nature alone cannot make up for what people have done. Dams in the Columbia River Basin have blocked huge areas of the wild salmon`s spawning grounds. Roads and towns sprouted up along rivers and streams. Logging and farming practices fouled rivers and creeks. So did pollution from the cities. And it became too easy to catch fish. Salmon runs became smaller and smaller. Some types of salmon disappeared forever. Having nearly destroyed the salmon, people are now coming to their rescue. Still, important runs of Northwest native salmon are in real danger of extinction. Much remains to be done. This brochure presents a close look at the life of a wild salmon, Oncorhynchus tshawystcha.

  14. Analysis of Leaf and Root Transcriptome of Soil Grown Avena barbata Plants

    SciTech Connect (OSTI)

    Swarbreck, St; phanie; Lindquist, Erika; Ackerly, David; Andersen, Gary

    2011-02-01

    Slender wild oat (Avena barbata) is an annual grass dominant in many grassland ecosystems in Mediterranean climate. This species has been the subject of ecological studies that aim at understanding the effect of global climate change on grassland ecosystems and the genetic basis for adaptation under varying environmental conditions. We present the sequencing and analysis of cDNA libraries constructed from leaf and root samples collected from A. barbata grown on natural soil and under varying rainfall patterns. More than one million expressed sequence tags (ESTs) were generated using both GS 454-FLX pyrosequencing and Sanger sequencing, and these tags were assembled into consensus sequences. We identified numerous candidate polymorphic markers in the dataset, providing possibilities for linking the genomic and the existing genetic information for A. barbata. Using the digital northern method, we showed that genes involved in photosynthesis were down regulated under high rainfall while stress- related genes were up regulated. We also identified a number of genes unique to the root library with unknown function. Real-time RT-PCR was used to confirm the root specificity of some of these transcripts such as two genes encoding O-methyl transferase. Also we showed differential expression under three water levels. Through a combination of Sanger and 454-based sequencing technologies, we were able to generate a large set of transcribed sequences for A. barbata. This dataset provides a platform for further studies of this important wild grass species

  15. Ecological Interactions Between Metals and Microbes That Impact Bioremediation

    SciTech Connect (OSTI)

    Konopka, Allan E.

    1998-06-01

    Samples have been obtained from (a) soil highly contaminated with Cr (tannery site) and (b) soils contaminated with petroleum, Cr, and Pb (Seymour, IN). Microcosm experiments with the tannery site soil indicated that microbial biomass (assayed as phospholipid-phosphate) and activity (assayed as carbon dioxide evolution) were primarily determined by organic carbon availability, but not total Cr concentration. The toxicity of metals to the indigenous microbial populations of the Seymour soils was determined by measuring microbial activity (incorporation of tritiated leucine into protein) of cells extracted from soil particles in solutions of increasing metal concentration. Although total Cr concentration varied 100-fold in these soils, the inhibition constant for Cr toxicity varied < 3-fold. Of additional interest in one soil was the dose-response function; the response suggests the soil contains a complex mixture of microbes with different Cr resistance levels. Cr and Pb resistant bacteria have been isolated from these soil samples. In Arthrobacter sp. Cr15, Cr resistance was spontaneously lost at a frequency of ca. 0.5% after growth for 20 generations in non-selective medium. The wild-type contained a 60 kb plasmid. In two Cr sensitive strains, restriction fragment analysis has shown that 15 kb of the plasmid have been lost. Matings between the wild type and cured strains result in transfer of the Cr resistance phenotype at a frequency of 1%.

  16. Uses of Ku70

    DOE Patents [OSTI]

    Li, Gloria C.; Cordon-Cardo, Carlos; Ouyang, Honghai

    2004-10-26

    This invention provides a method of diagnosing a predisposition to cancer in a subject comprising: (a) obtaining a nucleic acid sample from the subject; and; (b) determining whether one or more of the subject's Ku70 alleles or regulatory regions to those alleles are deleted or different from the wild type so as to reduce or eliminate the subject's expression of polypeptide having tumor suppressor activity. This invention also provides a method of assessing the severity of cancer in a subject comprising: (a) obtaining a nucleic acid sample from the subject; and (b) determining whether one or more of the subject's Ku70 alleles or regulatory regions to those alleles are deleted or different from the wild type so as to reduce or eliminate the subject's expression of polypeptide having tumor suppressor activity. This invention also provides a method of assessing the severity of cancer in a subject comprising: determining the subcellular localization of Ku70 in the subject, wherein an abnormal subcellular localization of Ku70 indicates a predisposition to cancer.

  17. Structure of adenovirus bound to cellular receptor car

    DOE Patents [OSTI]

    Freimuth, Paul I.

    2004-05-18

    Disclosed is a mutant adenovirus which has a genome comprising one or more mutations in sequences which encode the fiber protein knob domain wherein the mutation causes the encoded viral particle to have significantly weakened binding affinity for CARD1 relative to wild-type adenovirus. Such mutations may be in sequences which encode either the AB loop, or the HI loop of the fiber protein knob domain. Specific residues and mutations are described. Also disclosed is a method for generating a mutant adenovirus which is characterized by a receptor binding affinity or specificity which differs substantially from wild type. In the method, residues of the adenovirus fiber protein knob domain which are predicted to alter D1 binding when mutated, are identified from the crystal structure coordinates of the AD12knob:CAR-D1 complex. A mutation which alters one or more of the identified residues is introduced into the genome of the adenovirus to generate a mutant adenovirus. Whether or not the mutant produced exhibits altered adenovirus-CAR binding properties is then determined.

  18. Systems Level Approaches to Understanding and Manipulating Heterocyst Differentiation in Nostoc Punctiforme: Sites of Hydrogenase and Nitrogenase Synthesis and Activity

    SciTech Connect (OSTI)

    Meeks, John C.

    2015-04-02

    Heterocysts are specialized cells that establish a physiologically low oxygen concentration; they function as the sites of oxygen-sensitive nitrogen fixation and hydrogen metabolism in certain filamentous cyanobacteria. They are present at a frequency of less than 10% of the cells and singly in a nonrandom spacing pattern in the filaments. The extent of differential gene expression during heterocyst differentiation was defined by DNA microarray analysis in wild type and mutant cultures of Nostoc punctiforme. The results in wild-type cultures identified two groups of genes; approximately 440 that are unique to heterocyst formation and function, and 500 that respond positively and negatively to the transient stress of nitrogen starvation. Nitrogen fixation is initiated within 24 h after induction, but the cultures require another 24 h before growth is reinitiated. Microarray analyses were conducted on strains with altered expression of three genes that regulate the presence and spacing of heterocysts in the filaments; loss of function or over expression of these genes increases the heterocyst frequency 2 to 3 fold compared to the wild-type. Mutations in the genes hetR and hetF result in the inability to differentiate heterocysts, whereas over expression of each gene individually yields multiple contiguous heterocysts at sites in the filaments; they are positive regulatory elements. Mutation of the gene patN results in an increase in heterocysts frequency, but, in this case, the heterocysts are singly spaced in the filaments with a decrease in the number of vegetative cells in the interval between heterocysts; this is a negative regulatory element. However, over expression of patN resulted in the wild-type heterocyst frequency and spacing pattern. Microarray results indicated HetR and HetF influence the transcription of a common set of about 395 genes, as well as about 350 genes unique to each protein. HetR is known to be a transcriptional regulator and HetF is predicted to be a protease, perhaps operating thorough stability of HetR; thus, the influence of HetF on transcription of a unique set of genes was unanticipated. These two proteins are also found in non-heterocyst-forming filamentous cyanobacteria and the results have implications on their other physiological role(s). The PatN protein is unique to heterocyst-forming cyanobacteria. Cytological analysis indicated PatN is present in only one of the two daughter cells following division, but is present in both cell less than 8 h after division. Microarray analysis indicated only five genes were differentially transcribed in the patN mutant compared to the wild type; three up-regulated genes that are known to influence heterocyst differentiation and two down-regulated genes that have an unassigned function. Mutational analyses indicted the two down-regulated genes do not have a distinct role in heterocyst differentiation. Thus, PatN only indirectly impacts transcription. These databases provide lists of differentially transcribed genes involved in nitrogen starvation and cellular differentiation that can be mined for detailed genetic analysis of the regulation of heterocyst formation and function for subsequent photo-biohydrogen production.

  19. Survival Estimates for the Passage of Spring-Migrating Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2004-2005 Annual Report.

    SciTech Connect (OSTI)

    Smith, Steven G.; Muir, William D.; Marsh, Douglas M.

    2005-10-01

    In 2004, the National Marine Fisheries Service and the University of Washington completed the twelfth year of a study to estimate survival and travel time of juvenile salmonids (Oncorhynchus spp.) passing through dams and reservoirs on the Snake and Columbia Rivers. All estimates were derived from detections of fish tagged with passive integrated transponder tags (PIT tags). We PIT tagged and released a total of 19,621 hatchery steelhead, 8,128 wild steelhead, and 9,227 wild yearling Chinook salmon at Lower Granite Dam. In addition, we utilized fish PIT tagged by other agencies at traps and hatcheries upstream from the hydropower system and sites within the hydropower system. PIT-tagged smolts were detected at interrogation facilities at Lower Granite, Little Goose, Lower Monumental, McNary, John Day, and Bonneville Dams and in the PIT-tag detector trawl operated in the Columbia River estuary. Survival estimates were calculated using a statistical model for tag-recapture data from single release groups (the single-release model). Primary research objectives in 2004 were to (1) estimate reach survival and travel time in the Snake and Columbia Rivers throughout the migration period of yearling Chinook salmon O. tshawytscha and steelhead O. mykiss; (2) evaluate relationships between survival estimates and migration conditions; and (3) evaluate the survival-estimation models under prevailing conditions. This report provides reach survival and travel time estimates for 2004 for PIT-tagged yearling Chinook salmon (hatchery and wild), hatchery sockeye salmon O. nerka, hatchery coho salmon O. kisutch, and steelhead (hatchery and wild) in the Snake and Columbia Rivers. Results are reported primarily in the form of tables and figures; details on methodology and statistical models used are provided in previous reports cited here. Survival and detection probabilities were estimated precisely for most of the 2004 yearling Chinook salmon and steelhead migrations. Hatchery and wild fish were combined in some of the analyses. Overall, the percentages for combined release groups used in survival analyses were 68% hatchery-reared yearling Chinook salmon and 32% wild. For steelhead, the overall percentages were 73% hatchery-reared and 27% wild. Estimated survival from the tailrace of Lower Granite Dam to the tailrace of Little Goose Dam averaged 0.923 for yearling Chinook salmon and 0.860 for steelhead. Respective average survival estimates for yearling Chinook salmon and steelhead were 0.875 and 0.820 from Little Goose Dam tailrace to Lower Monumental Dam tailrace; 0.818 and 0.519 from Lower Monumental Dam tailrace to McNary Dam tailrace (including passage through Ice Harbor Dam); and 0.809 and 0.465 from McNary Dam tailrace to John Day Dam tailrace. Survival for yearling Chinook salmon from John Day Dam tailrace to Bonneville Dam tailrace (including passage through The Dalles Dam) was 0.735. We were unable to estimate survival through this reach for steelhead during 2004 because too few fish were detected at Bonneville Dam due to operation of the new corner collector at the second powerhouse. Combining average estimates from the Snake River smolt trap to Lower Granite Dam, from Lower Granite Dam to McNary Dam, and from McNary Dam to Bonneville Dam, estimated annual average survival through the entire hydropower system from the head of Lower Granite reservoir to the tailrace of Bonneville Dam (eight projects) was 0.353 (s.e. 0.045) for Snake River yearling Chinook salmon. We could not empirically estimate survival through the entire system for steelhead in 2004 because of low detection rates for this species at Bonneville Dam. For yearling spring Chinook salmon released in the Upper Columbia River, estimated survival from point of release to McNary Dam tailrace was 0.484 (s.e. 0.005) for fish released from Leavenworth Hatchery, 0.748 (s.e. 0.015) for fish released from Entiat Hatchery, 0.738 (s.e. 0.036) for fish released from Winthrop Hatchery, and 0.702 (s.e. 0.048) and 0.747 (s.e.0.047) for those from Methow Hatchery, Chewuch Pond and

  20. Yakima River Spring Chinook Enhancement Study, 1987 Annual Report.

    SciTech Connect (OSTI)

    Fast, David E.

    1988-01-01

    The smelt outmigration was monitored at wapatox on the Naches River and Prosser on the lower Yakima. The spring outmigration at Wapatox was estimated to be 16,141 smolts. The 1987 spring outmigration of wild spring chinook from the Yakima Basin was estimated to be 251,975 smolts at Prosser. The survival from egg to smelt was calculated using the 1985 redd counts and the 1987 smolt outmigration at Prosser. The estimated survival was 4.16%, which gives a mean egg to smolt survival over four years of 6.32%. In 1987 a total of 3,683 adult and 335 jack spring chinook salmon returning to the Yakima River were counted at Prosser fish ladder. This gives a total of 4,018 salmon returning to Prosser Dam. The median dates of passage were May 12 and May 16 for adults and jacks respectively. An additional 372 fish were estimated to have been caught in the Yakima River subsistence dipnet fishery below Horn Rapids and Prosser Dams. Therefore, total return to the Yakima system was 4,390 spring chinook salmon. Spring chinook were counted at Roza Dam from May 1 to September 30, 1987. Passage at Roza Dam was 1,610 adult and 67 jack spring chinook for a total of 1,677 wild fish. The median dates of passage at Roza Dam were May 29 and May 26 for spring chinook adults and jacks respectively. The smolt to adult (S{sub sa}) survival was calculated based on the 1983 smelt outmigration estimated at Prosser and the 1984 return of jacks (3 year old fish) the 1985 return of four year old adults, and the 1986 return of five year old fish to the Yakima River. It was estimated that 6,012 wild three, four, and five year old fish returned from an estimated smolt outmigration of 135,548 fish in 1983. This gives an estimated survival from smolt to adult of 4.4%. The smolt to adult survival for the 1984 smolt outmigration was 5.3% with 423 jacks returning in 1985, 5,163 four year old adults returning in 1986, and 983 five year old fish returning in 1987 fran an estimated 123,732 smolts in 1984. Spring chinook adults from fourteen different hatchery release groups were recovered in 1987. A total of 211 coded wire tags were recovered and these were expanded to an estimated 253 returning hatchery fish in 1987. Nine of these fish were jacks.

  1. International low level waste disposal practices and facilities

    SciTech Connect (OSTI)

    Nutt, W.M.

    2011-12-19

    The safe management of nuclear waste arising from nuclear activities is an issue of great importance for the protection of human health and the environment now and in the future. The primary goal of this report is to identify the current situation and practices being utilized across the globe to manage and store low and intermediate level radioactive waste. The countries included in this report were selected based on their nuclear power capabilities and involvement in the nuclear fuel cycle. This report highlights the nuclear waste management laws and regulations, current disposal practices, and future plans for facilities of the selected international nuclear countries. For each country presented, background information and the history of nuclear facilities are also summarized to frame the country's nuclear activities and set stage for the management practices employed. The production of nuclear energy, including all the steps in the nuclear fuel cycle, results in the generation of radioactive waste. However, radioactive waste may also be generated by other activities such as medical, laboratory, research institution, or industrial use of radioisotopes and sealed radiation sources, defense and weapons programs, and processing (mostly large scale) of mineral ores or other materials containing naturally occurring radionuclides. Radioactive waste also arises from intervention activities, which are necessary after accidents or to remediate areas affected by past practices. The radioactive waste generated arises in a wide range of physical, chemical, and radiological forms. It may be solid, liquid, or gaseous. Levels of activity concentration can vary from extremely high, such as levels associated with spent fuel and residues from fuel reprocessing, to very low, for instance those associated with radioisotope applications. Equally broad is the spectrum of half-lives of the radionuclides contained in the waste. These differences result in an equally wide variety of options for the management of radioactive waste. There is a variety of alternatives for processing waste and for short term or long term storage prior to disposal. Likewise, there are various alternatives currently in use across the globe for the safe disposal of waste, ranging from near surface to geological disposal, depending on the specific classification of the waste. At present, there appears to be a clear and unequivocal understanding that each country is ethically and legally responsible for its own wastes, in accordance with the provisions of the Joint Convention on the Safety of Spent Fuel Management and on the Safety of Radioactive Waste Management. Therefore the default position is that all nuclear wastes will be disposed of in each of the 40 or so countries concerned with nuclear power generation or part of the fuel cycle. To illustrate the global distribution of radioactive waste now and in the near future, Table 1 provides the regional breakdown, based on the UN classification of the world in regions illustrated in Figure 1, of nuclear power reactors in operation and under construction worldwide. In summary, 31 countries operate 433 plants, with a total capacity of more than 365 gigawatts of electrical energy (GW[e]). A further 65 units, totaling nearly 63 GW(e), are under construction across 15 of these nations. In addition, 65 countries are expressing new interest in, considering, or actively planning for nuclear power to help address growing energy demands to fuel economic growth and development, climate change concerns, and volatile fossil fuel prices. Of these 65 new countries, 21 are in Asia and the Pacific region, 21 are from the Africa region, 12 are in Europe (mostly Eastern Europe), and 11 in Central and South America. However, 31 of these 65 are not currently planning to build reactors, and 17 of those 31 have grids of less than 5 GW, which is said to be too small to accommodate most of the reactor designs available. For the remaining 34 countries actively planning reactors, as of September 2010: 14 indicate a strong intention to precede with introduction of nuclear power; 7 are preparing but haven't made a final decision, 10 have made a decision and are preparing infrastructure, 2 have ordered a new nuclear power plant, and 1 has a plant under construction. In all countries interested in pursuing nuclear power, it is necessary for the governments to create an environment for investment and advancement of nuclear power, including development of a professional and independent regulatory framework and regime, knowledge and refinement of skills in nuclear safety and control, definition of policies on nuclear waste management and decommissioning, and participation in international non-proliferation measures. Specifically related to radioactive waste management, nuclear facilities and industries that utilize radioactive material work to well-established safety standards for the management of their waste.

  2. Distribution of Polycyclic Aromatic Hydrocarbons in Soils and Terrestrial Biota After a Spill of Crude Oil in Trecate, Italy

    SciTech Connect (OSTI)

    Brandt, Charles A. ); Becker, James M. ); Porta, Augusto C.

    2001-12-01

    Following a large blowout of crude oil in northern Italy in 1994, the distribution of polyaromatic hydrocarbons (PAHs) was examined over time and space in soils, uncultivated wild vegetation, insects, mice, and frogs in the area. Within 2 y of the blowout, PAH concentrations declined to background levels over much of the area where initial concentrations were within an order of magnitude above background, but had not declined to background in areas where starting concentrations exceeded background by two orders of magnitude. Octanol-water partitioning and extent of alkylation explained much of the variance in uptake of PAHs by plants and animals. Lower Kow PAHs and higher-alkylated PAHs had higher soil-to-biota accumulation factors (BSAFs) than did high-Kow and unalkylated forms. BSAFs for higher Kow PAHs were very low for plants, but much higher for animals, with frogs accumulating more of these compounds than other species.

  3. Formation of mushrooms and lignocellulose degradation encoded in the genome sequence of Schizophyllum commune

    SciTech Connect (OSTI)

    Ohm, Robin A.; de Jong, Jan F.; Lugones, Luis G.; Aerts, Andrea; Kothe, Erika; Stajich, Jason E.; de Vries, Ronald P.; Record, Eric; Levasseur, Anthony; Baker, Scott E.; Bartholomew, Kirk A.; Coutinho, Pedro M.; Erdmann, Susann; Fowler, Thomas J.; Gathman, Allen C.; Lombard, Vincent; Henrissat, Bernard; Knabe, Nicole; Kues, Ursula; Lilly, Walt W.; Lindquist, Erika; Lucas, Susan; Magnuson, Jon K.; Piumi, Francois; Raudaskoski, Marjatta; Salamov, Asaf; Schmutz, Jeremy; Schwarze, Francis W.M.R.; van Kuyk, Patricia A.; Horton, J. Stephen; Grigoriev, Igor V.; Wosten, Han A.B.

    2010-07-12

    The wood degrading fungus Schizophyllum commune is a model system for mushroom development. Here, we describe the 38.5 Mb assembled genome of this basidiomycete and application of whole genome expression analysis to study the 13,210 predicted genes. Comparative analyses of the S. commune genome revealed unique wood degrading machinery and mating type loci with the highest number of reported genes. Gene expression analyses revealed that one third of the 471 identified transcription factor genes were differentially expressed during sexual development. Two of these transcription factor genes were deleted. Inactivation of fst4 resulted in the inability to form mushrooms, whereas inactivation of fst3 resulted in more but smaller mushrooms than wild-type. These data illustrate that mechanisms underlying mushroom formation can be dissected using S. commune as a model. This will impact commercial production of mushrooms and the industrial use of these fruiting bodies to produce enzymes and pharmaceuticals.

  4. Genome sequence analysis of the model grass Brachypodium distachyon: insights into grass genome evolution

    SciTech Connect (OSTI)

    Schulman, Al

    2009-08-09

    Three subfamilies of grasses, the Erhardtoideae (rice), the Panicoideae (maize, sorghum, sugar cane and millet), and the Pooideae (wheat, barley and cool season forage grasses) provide the basis of human nutrition and are poised to become major sources of renewable energy. Here we describe the complete genome sequence of the wild grass Brachypodium distachyon (Brachypodium), the first member of the Pooideae subfamily to be completely sequenced. Comparison of the Brachypodium, rice and sorghum genomes reveals a precise sequence- based history of genome evolution across a broad diversity of the grass family and identifies nested insertions of whole chromosomes into centromeric regions as a predominant mechanism driving chromosome evolution in the grasses. The relatively compact genome of Brachypodium is maintained by a balance of retroelement replication and loss. The complete genome sequence of Brachypodium, coupled to its exceptional promise as a model system for grass research, will support the development of new energy and food crops

  5. Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Amore, Antonella; Iadonisi, Alfonso; Vincent, Florence; Faraco, Vincenza

    2015-12-21

    In this paper, the recombinant α-l-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleophilic residue. Thus, the mutant E449G of PoAbf was recombinantly expressed in Pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. The design of a suitable set of hydrolysis experiments in the presence or absence of alcoholic arabinosyl acceptors and/or formate salts allowed to unambiguously identify the residue E449 as the nucleophile residue involvedmore » in the retaining mechanism of this GH51 arabinofuranosidase. 1H NMR analysis was applied for the identification of the products and the assignement of their anomeric configuration.« less

  6. Molecular ageing: Free radical initiated epimerization of thymopentin A case study

    SciTech Connect (OSTI)

    Sheykhkarimli, Dayag; Choo, Ken-Loon; Owen, Michael; Csizmadia, Imre G.; Fiser, Bla; Jjrt, Balzs; Viskolcz, Bla

    2014-05-28

    The epimerization of amino acid residues increases with age in living organisms. In the present study, the structural consequences and thermodynamic functions of the epimerization of thymopentin (TP-5), the active site of the thymic hormone thymopoietin, were studied using molecular dynamics and density functional theory methods. The results show that free radical-initiated D-amino acid formation is energetically favoured (?130 kJmol{sup ?1}) for each residue and induces significant changes to the peptide structure. In comparison to the wild-type (each residue in the L-configuration), the radius of gyration of the D-Asp{sup 3} epimer of the peptide decreased by 0.5 , and disrupted the intramolecular hydrogen bonding of the native peptide. Beyond establishing important structural, energetic and thermodynamic benchmarks and reference data for the structure of TP-5, these results disseminate the understanding of molecular ageing, the epimerization of amino acid residues.

  7. Willamette Hatchery Oxygen Supplementation Studies : Annual Report 1993.

    SciTech Connect (OSTI)

    Ewing, R.D.; Ewing, S.K.; Sheahan, J.E.

    1993-11-01

    Hydropower development and operations in the Columbia River basin have caused the loss of 5 million to 11 million salmonids. An interim goal of the Northwest Power Planning Council is to reestablish these historical numbers by doubling the present adult runs from 2.5 million to 5.0 million fish. This increase in production will be accomplished through comprehensive management of both wild and hatchery fish, but artificial propagation will play a major role in the augmentation process. The current husbandry techniques in existing hatcheries require improvements that may include changes in rearing densities, addition of oxygen, removal of excess nitrogen, and improvement in raceway design. Emphasis will be placed on the ability to increase the number of fish released from hatcheries that survive to return as adults.

  8. Sandwiched Rnyi divergence satisfies data processing inequality

    SciTech Connect (OSTI)

    Beigi, Salman [School of Mathematics, Institute for Research in Fundamental Sciences (IPM), P.O. Box 19395-5746, Tehran (Iran, Islamic Republic of)] [School of Mathematics, Institute for Research in Fundamental Sciences (IPM), P.O. Box 19395-5746, Tehran (Iran, Islamic Republic of)

    2013-12-15

    Sandwiched (quantum) ?-Rnyi divergence has been recently defined in the independent works of Wilde et al. [Strong converse for the classical capacity of entanglement-breaking channels, preprint http://arxiv.org/abs/arXiv:1306.1586 (2013)] and Mller-Lennert et al. [On quantum Rnyi entropies: a new definition, some properties and several conjectures, preprint http://arxiv.org/abs/arXiv:1306.3142v1 (2013)]. This new quantum divergence has already found applications in quantum information theory. Here we further investigate properties of this new quantum divergence. In particular, we show that sandwiched ?-Rnyi divergence satisfies the data processing inequality for all values of ? > 1. Moreover we prove that ?-Holevo information, a variant of Holevo information defined in terms of sandwiched ?-Rnyi divergence, is super-additive. Our results are based on Hlder's inequality, the Riesz-Thorin theorem and ideas from the theory of complex interpolation. We also employ Sion's minimax theorem.

  9. Optimal measurement of surface shortwave irradiance using current instrumentation -- the ARM experience

    SciTech Connect (OSTI)

    Michalsky, J.; Rubes, M.; Stoffel, T.; Wesley, M.; Splitt, M.; DeLuisi, J.

    1997-03-01

    Shortwave (solar) measurements of surface irradiance for clear sky conditions disagree with a number of different models. Betts used the European Center for Medium-range Forecasts (ECMWF) shortwave model to calculate surface irradiance that were 5-10 percent higher than measurements. Wild used a different formulation of the ECMWF shortwave model, but found that the model overpredicted clear-sky shortwave and average of 3 percent. Ding and Wang used data from the Atmospheric Radiation Measurement (ARM) program and found that the GENESIS GCM shortwave model, likewise, overpredicted clear-sky irradiance by about 4 percent. To help resolve the measurement dilemma, reference instruments were deployed in April 1996 at the Southern Great Plains ARM site central facility very near the shortwave measurements. The rest of the paper describes the experiment undertaken to ascertain total horizontal shortwave irradiance at the surface, including a separation of the direct normal and diffuse horizontal components. Results and a discussion of same concludes the paper.

  10. Real-Time Water Quality Monitoring and Habitat Assessment in theSan Luis National Wildlife Refuge

    SciTech Connect (OSTI)

    Quinn, Nigel W.T.; Hanlon, Jeremy S.; Burns, Josephine R.; Stromayer, Karl A.K.; Jordan, Brandon M.; Ennis, Mike J.; Woolington,Dennis W.

    2005-08-28

    The project report describes a two year experiment to control wetland drainage to the San Joaquin River of California from the San Luis National Wildlife Refuge using a decision support system for real-time water quality management. This system required the installation and operation of one inlet and three drainage flow and water quality monitoring stations which allowed a simple mass balance model to be developed of the seasonally managed wetlands in the study area. Remote sensing methods were developed to document long-term trends in wetland moist soil vegetation and soil salinity in response to management options such as delaying the initiation of seasonal wetland drainage. These environmental management tools provide wetland managers with some of the tools necessary to improve salinity conditions in the San Joaquin River and improve compliance with State mandated salinity objectives without inflicting long-term harm on the wild fowl habitat resource.

  11. Monitoring the Reproductive Success of Naturally Spawning Hatchery and Natural Spring Chinook Salmon in the Wenatchee River, 2008-2009 Progress Report.

    SciTech Connect (OSTI)

    Ford, Michael J.; Williamson, Kevin S.

    2009-05-28

    We investigated differences in the statistical power to assign parentage between an artificially propagated and wild salmon population. The propagated fish were derived from the wild population, and are used to supplement its abundance. Levels of genetic variation were similar between the propagated and wild groups at 11 microsatellite loci, and exclusion probabilities were >0.999999 for both groups. The ability to unambiguously identify a pair of parents for each sampled progeny was much lower than expected, however. Simulations demonstrated that the proportion of cases the most likely pair of parents were the true parents was lower for propagated parents than for wild parents. There was a clear relationship between parentage assignment ability and the degree of linkage disequilibrium, the estimated effective number of breeders that produced the parents, and the size of the largest family within the potential parents. If a stringent threshold for parentage assignment was used, estimates of relative fitness were biased downward for the propagated fish. The bias appeared to be largely eliminated by either fractionally assigning progeny among parents in proportion to their likelihood of parentage, or by assigning progeny to the most likely set of parents without using a statistical threshold. We used a DNA-based parentage analysis to measure the relative reproductive success of hatchery- and natural-origin spring Chinook salmon in the natural environment. Both male and female hatchery-origin fish produced far fewer juvenile progeny per parent when spawning naturally than did natural origin fish. Differences in age structure, spawning location, weight and run timing were responsible for some of the difference in fitness. Male size and age had a large influence on fitness, with larger and older males producing more offspring than smaller or younger individuals. Female size had a significant effect on fitness, but the effect was much smaller than the effect of size on male fitness. For both sexes, run time had a smaller but still significant effect on fitness, with earlier returning fish favored. Spawning location within the river had a significant effect on fitness for both males and females, and for females explained most of the reduced fitness observed for hatchery fish in this population. While differences have been reported in the relative reproductive success of hatchery and naturally produced salmonids Oncorhynchus spp., factors explaining the differences are often confounded. We examined the spawning site habitat and redd structure variables of hatchery and naturally produced spring Chinook salmon O. tshawytscha of known size that spawned in two tributaries of the Wenatchee River. We controlled for variability in spawning habitat by limiting our analysis to redds found within four selected reaches. No difference in the instantaneous spawner density or location of the redd in the stream channel was detected between reaches. Within each reach, no difference in the fork length or weight of hatchery and naturally produced fish was detected. While most variables differed between reaches, we found no difference in redd characteristics within a reach between hatchery and naturally produced females. Correlation analysis of fish size and redd characteristics found several weak but significant relationships suggesting larger fish contract larger redds in deeper water. Spawner density was inversely related to several redd structure variables suggesting redd size may decrease as spawner density increases. Results should be considered preliminary until samples size and statistical power goals are reached in future years. Trends in relative reproductive success of hatchery and naturally produced spring Chinook salmon Oncorhynchus tshawytscha in the Wenatchee Basins suggest females that spawn in the upper reaches of the tributaries produced a great number of offspring compared to females that spawn in the lower reaches of the tributaries. To better understand this trend, redd microhabitat data was collected from spring Chinook sa

  12. Rocky Mountain Basins Produced Water Database

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Historical records for produced water data were collected from multiple sources, including Amoco, British Petroleum, Anadarko Petroleum Corporation, United States Geological Survey (USGS), Wyoming Oil and Gas Commission (WOGC), Denver Earth Resources Library (DERL), Bill Barrett Corporation, Stone Energy, and other operators. In addition, 86 new samples were collected during the summers of 2003 and 2004 from the following areas: Waltman-Cave Gulch, Pinedale, Tablerock and Wild Rose. Samples were tested for standard seven component "Stiff analyses", and strontium and oxygen isotopes. 16,035 analyses were winnowed to 8028 unique records for 3276 wells after a data screening process was completed. [Copied from the Readme document in the zipped file available at http://www.netl.doe.gov/technologies/oil-gas/Software/database.html] Save the Zipped file to your PC. When opened, it will contain four versions of the database: ACCESS, EXCEL, DBF, and CSV formats. The information consists of detailed water analyses from basins in the Rocky Mountain region.

  13. Economic evaluation of rural woodlots in a developing country: Tanzania

    SciTech Connect (OSTI)

    Kihiyo, V.B.M.S.

    1996-03-01

    Rural areas in developing countries use wood as their main source of energy. Previously, wood has been obtained free from natural forests and woodlands. The pressure of increased demand through population growth, and the fact that natural trees take longer to grow, has made this resource scarce. Thus, raising trees in woodlots has been adopted as the solution to its shortage in the wild. However, growing trees in woodlots will inevitably require resources in terms of capital, land and manpower. Economic evaluation becomes necessary to ascertain that these resources are used economically. This paper dwells on some of the salient features of the economic evaluation of woodlots, such as interest rates, shadow prices of factors of production, social opportunity, cost of capital and sensitivity analysis of such woodlots in a developing country such as Tanzania. 19 refs., 5 tabs.

  14. Kelt Reconditioning: A Research Project to Enhance Iteroparity in Columbia Basin Steelhead (Oncorhynchus mykiss), 2004 Annual Report.

    SciTech Connect (OSTI)

    Hatch, Douglas R.; Branstetter, Ryan; Whiteaker, John

    2004-11-01

    Iteroparity, the ability to repeat spawn, is a life history strategy that is expressed by some species from the family Salmonidae. Rates of repeat spawning for post-development Columbia River steelhead Oncorhynchus mykiss populations range from 1.6 to 17%. It is expected that currently observed iteroparity rates for wild steelhead in the Basin are severely depressed due to development and operation of the hydropower system and various additional anthropogenic factors. Increasing the expression of historical repeat spawning rates using fish culturing methods could be a viable technique to assist the recovery of depressed steelhead populations, and could help reestablish this naturally occurring life history trait. Reconditioning is the process of culturing post-spawned fish (kelts) in a captive environment until they are able to reinitiate feeding, growth, and redevelop mature gonads. Kelt reconditioning techniques were initially developed for Atlantic salmon Salmo salar and sea-trout S. trutta. The recent Endangered Species Act listing of many Columbia River Basin steelhead populations has prompted interest in developing reconditioning methods for wild steelhead populations within the Basin. To test kelt steelhead reconditioning as a potential recovery tool, wild emigrating steelhead kelts were placed into one of three study groups (direct capture and transport, short-term reconditioning, or long-term reconditioning). Steelhead kelts from the Yakima River were collected at the Chandler Juvenile Monitoring Facility (CJMF, located on the Yakima River at river kilometer 75.6) from 15 March to 21 June 2004. In total, 842 kelts were collected for reconditioning at Prosser Hatchery. Captive specimens represented 30.5% (842 of 2,755) of the entire 2003-2004 Yakima River wild steelhead population, based on fish ladder counts at Prosser Dam. All steelhead kelts were reconditioned in 20-foot circular tanks, and fed freeze-dried krill initially or for the duration of the experiment. All steelhead kelts received hw-wiegandt multi vit dietary supplement as a means to improve initial nutrition. Long-term steelhead kelts received Moore-Clark pellets to provide essential minerals and nutrients necessary for gonadal redevelopment. Oxytetracycline was administered to all reconditioned fish to boost immune system response following the stress of initial capture. To control parasitic infestations two methods were used, first, after initial capture an intubation of Ivermectin{trademark} was administered to control internal parasites (e.g., Salmincola spp.). Next, a Formalin drip was used for the duration of reconditioning to prevent fungal outbreaks. Captured kelts were separated into three experimental groups: short-term reconditioning, long-term reconditioning, and direct transport and release. Success indicators for the short-term experiment include the proportion of fish that survived the reconditioning process and the proportion of fish that initiated a feeding response. Short-term kelts were reconditioned for 3 to 5 weeks. Surviving specimens were released for natural spawning on May 11, 2004. Survival-to-release was good for the short-term experiment, with a rate of 79.0%. Long-term steelhead kelts are currently being held for a 6-9 month period with a scheduled release in December 2004. Long-term success indicators include the proportion of fish that survived the reconditioning process and the proportion of surviving fish that successfully remature. Survival and rematuration for long-term kelts has not been determined and will be presented in the 2005 annual report. Direct transport and release kelts and short-term reconditioned kelts were radio or acoustic tagged to assess their travel time and migratory behaviors below Bonneville Dam. A total of 29 direct-transport and release kelts and 29 short-term reconditioned kelts received surgically implanted radio tags, and a total of 28 direct-transport/release and 26 short-term reconditioned fish received surgically implanted hydro acoustic tags. These tags will allow us to determine outm

  15. Identifying Event Impacts by Monitoring the News Media

    SciTech Connect (OSTI)

    Patton, Robert M; Potok, Thomas E

    2008-01-01

    Assessing the potential property and social impacts of an event, such as tornado or wildfire, continues to be a challenging research area. From financial markets to disaster management to epidemiology, the importance of understanding the impacts that events create cannot be understated. Our work describes an approach to fuse information from multiple sources, then to analyze the information cycles to identify prior temporal patterns related to the impact of an event. This approach is then applied to the analysis of news reports from multiple news sources pertaining to several different natural disasters. Results show that our approach can project the severity of the impacts of certain natural disasters, such as heat waves on droughts and wild fires. In addition, results show that specific types of disaster consistently produce similar impacts when each time they occur.

  16. Constraints on the Formation Age of Cometary Material from the NASA Stardust Mission

    SciTech Connect (OSTI)

    Matzel, J; Ishii, H; Joswiak, D; Hutcheon, I; Bradley, J; Brownlee, D; Weber, P K; Teslich, N; Matrajt, G; McKeegan, K; MacPherson, G

    2009-11-13

    We measured the {sup 26}Al-{sup 26}Mg isotope systematics of a {approx} 5-micrometer refractory particle, Coki, returned from comet 81P/Wild 2 in order to relate the time scales of formation of cometary inclusions to their meteoritic counterparts. The data show no evidence of radiogenic {sup 26}Mg and define an upper limit to the abundance of {sup 26}Al at the time of particle formation: {sup 26}Al/{sup 27}Al < 1 x 10-5. The absence of {sup 26}Al indicates that Coki formed >1.7 million years after the oldest solids in the solar system, calcium- and aluminum-rich inclusions (CAIs). The data suggest that high-temperature inner solar system material formed, was subsequently transferred to the Kuiper Belt, and was incorporated into comets several million years after CAI formation.

  17. EIS-0163-S: Supplemental EIS/1993 Interim Columbia and Snake Rivers Flow Improvement Measures for Salmon

    Broader source: Energy.gov [DOE]

    The U.S. Army Corps of Engineers – Walla Walla District has prepared this statement to assess alternatives to improve flows of water in the lower Columbia-Snake rivers in 1993 and future years to assist the migration of juvenile and adult anadromous fish past eight hydropower dams. The U.S. Department of Energy’s Bonneville Power Administration served as a cooperating agency in developing this supplement due to its key role in direct operation of the integrated and coordinated Columbia-Snake River System, and adopted this statement in March of 1993. This statement supplements the 1992 Columbia River Salmon Flow Measures Options Analysis Environmental Impact Statement, which evaluated ways to alter water management operations in 1992 on the lower Columbia and Snake rivers to enhance the survival of wild Snake River salmon.

  18. Comparative Survival Study (CSS) of Hatchery PIT-tagged Spring/Summer Chinook; Migration Years 1997-2002 Mark/Recapture Activities and Bootstrap Analysis, 2003-2004 Biennial Report.

    SciTech Connect (OSTI)

    Berggren, Thomas J.; Franzoni, Henry; Basham, Larry R.

    2003-11-01

    The Comparative Survival Study (CSS) was initiated in 1996 as a multi-year program of the fishery agencies and tribes to estimate survival rates over different life stages for spring and summer Chinook (hereafter, Chinook) produced in major hatcheries in the Snake River basin and from selected hatcheries in the lower Columbia River. Much of the information evaluated in the CSS is derived from fish tagged with Passive Integrated Transponder (PIT) tags. A comparison of survival rates of Chinook marked in two different regions (which differ in the number of dams Chinook have to migrate through) provides insight into the effects of the Snake/Columbia hydroelectric system (hydrosystem). The CSS also compares the smolt-to-adult survival rates (SARs) for Snake River Chinook that were transported versus those that migrated in-river to below Bonneville Dam. Additional comparisons can be made within in-river experiences as well as comparison between the different collector projects from which smolts are transported. CSS also compares survival rates for wild Snake River spring and summer Chinook. These comparisons generate information regarding the relative effects of the current management actions used to recover this listed species. Scientists and managers have recently emphasized the importance of delayed hydrosystem mortality to long-term management decisions. Delayed hydrosystem mortality may be related to the smolts experience in the Federal Columbia River Power System, and could occur for both smolts that migrate in-river and smolts that are transported. The CSS PIT tag information on in-river survival rates and smolt-to-adult survival rates (SARs) of transported and in-river fish are relevant to estimation of ''D'', which partially describes delayed hydrosystem mortality. The parameter D is the differential survival rate of transported fish relative to fish that migrate in-river, as measured from below Bonneville Dam to adults returning to Lower Granite Dam. When D = 1, there is no difference in survival rate after hydrosystem passage. When D < 1, then transported smolts die at a greater rate after release below Bonneville Dam than smolts that have migrated in-river to below Bonneville Dam Major objectives of the CSS include: (1) development of a long-term index of transport SAR to in-river SAR for Snake River hatchery and wild spring and summer Chinook smolts measured at Lower Granite Dam; (2) develop a long-term index of survival rates from release of smolts at Snake River hatcheries to return of adults to the hatcheries; (3) compute and compare the overall SARs for selected upriver and downriver spring and summer Chinook hatchery and wild stocks; and (4) begin a time series of SARs for use in hypothesis testing and in the regional long-term monitoring and evaluation program. Primary CSS focus in this report is for wild and hatchery spring/summer Chinook that outmigrated in 1997 to 2002 and their respective adult returns through 2004.

  19. Hood River and Pelton Ladder Evaluation Studies, Annual Report 2000-2001.

    SciTech Connect (OSTI)

    Olsen, Erik

    2009-09-01

    The Bonneville Power Administration (BPA) funded the development of two master plans which outline the rationale, and general approach, for implementing a defined group of projects that are an integral part of a comprehensive watershed goal to 'Protect, enhance and restore wild and natural populations of anadromous and resident fish within the Hood River Subbasin'. The Hood River Production Master Plan and the Pelton Ladder Master Plan were completed in 1991 and subsequently approved by the Northwest Power Planning Council in 1992. Action items identified in the two master plans, as well as in a later document entitled 'Hood River/Pelton Ladder Master Agreement' (ODFW and CTWSRO Undated), are designed to achieve two biological fish objectives: (1) to increase production of wild summer and winter steelhead (Oncorhynchus mykiss) to levels commensurate with the subbasins current carrying capacity and (2) re-establishing a self-sustaining population of spring chinook salmon (Oncorhynchus tshawytscha). Numerical fish objectives for subbasin escapement, spawner escapement, and subbasin harvest are defined for each of these species in Coccoli (2000). Several projects are presently funded by the BPA to achieve the Hood River subbasin's numerical fish objectives for summer and winter steelhead and spring chinook salmon. They include BPA project numbers 1998-021-00 (Hood River Fish Habitat), 1998-053-03 (Hood River Production Program - CTWSRO: M&E), 1998-053-07 (Parkdale Fish Facility), 1998-053-08 (Powerdale/Oak Springs O&M), and 1998-053-12 (Hood River Steelhead Genetics Study). Collectively, they are implemented under the umbrella of what has come to be defined as the Hood River Production Program (HRPP). The HRPP is jointly implemented by the Oregon Department of Fish and Wildlife (ODFW) and The Confederated Tribes of the Warm Springs Reservation of Oregon (CTWSRO). Strategies for achieving the HRPP's biological fish objectives for the Hood River subbasin were initially devised based on various assumptions about (1) subbasin carrying capacity, (2) survival rates for selected life history stages, and (3) historic and current escapements of wild, natural, and hatchery stocks of anadromous salmonids to the Hood River subbasin. The Oregon Department of Fish and Wildlife began funding a monitoring and evaluation (M&E) project in December 1991 to collect the quantitative biological information needed to (1) more accurately assess the validity of these assumptions and (2) evaluate the proposed hatchery supplementation component of the HRPP. Bonneville Power Administration assumed funding of the M&E project in August 1992. The M&E project was initially confined to sampling anadromous salmonids escaping to an adult trapping facility operated at Powerdale Dam; which is located at River Mile (RM) 4.5 on the mainstem of the Hood River. Stock specific life history and biological data was collected to (1) monitor subbasin spawner escapements and (2) collect pre-implementation data critical to evaluating the newly proposed HRPP's potential biological impact on indigenous populations of resident fish. The scope of the M&E project was expanded in 1994 to collect the data needed to quantify (1) subbasin smolt production and carrying capacity, (2) smolt to adult survival rates, and (3) the spatial distribution of indigenous populations of summer and winter steelhead, spring and fall chinook salmon, and coho salmon. A creel was incorporated into the M&E project in December 1996 to evaluate the HRPP with respect to its defined subbasin and spawner escapement objectives for Hood River stocks of wild and hatchery summer and winter steelhead and for natural and Deschutes stock hatchery spring chinook salmon. In 1996, the M&E project also began monitoring streamflow at various locations in the Hood River subbasin. Streamflow data will be used to correlate subbasin smolt production with summer streamflows. Data collected from 1991-1999 is reported in the following annual progress reports: Olsen et al. (1994), Olsen et al

  20. DISCLAIMER

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lawrence Livermore National Laboratory U.S. Department of Energy Approved for public release; further dissemination unlimited UCRL-ID-151619 Results from the First 249 Cf+ 48 Ca Experiment Y.T. Oganessian, V.K. Utyonkov, Y.V. Lobanov, F.S. Abdullin, A.N. Polyakov, I.V. Shirokovsky, Y.S. Tsyganov, A.N. Mezentsev, S. Iliev, V.G. Subbotin, A.M. Sukhov, O.V. Ivanov, A.A. Voinov, K. Subotic, V.I. Zagrebaev, M.G. Itkis, K.J. Moody, J.F. Wild, M.A. Stoyer, N.J. Stoyer, C.A. Laue, D.A. Shaughnessy, J.B.

  1. Sabin-to-Mahoney Transition Model of Quasispecies Replication

    Energy Science and Technology Software Center (OSTI)

    2009-05-31

    Qspp is an agent-based stochastic simulation model of the Poliovirus Sabin-to-Mahoney transition. This code simulates a cell-to-cell model of Poliovirus replication. The model tracks genotypes (virus genomes) as they are replicated in cells, and as the cells burst and release particles into the medium of a culture dish. An inoculum is then taken from the pool of virions and is used to inoculate cells on a new dish. This process repeats. The Sabin genotype comprisesmore » the initial inoculum. Nucleotide positions that match the Sabin1 (vaccine strain) and Mahoney (wild type) genotypes, as well as the neurovirulent phenotype (from the literature) are enumerated as constants.« less

  2. Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis

    SciTech Connect (OSTI)

    Pomraning, Kyle R.; Wei, Siwei; Karagiosis, Sue A.; Kim, Young-Mo; Dohnalkova, Alice; Arey, Bruce W.; Bredeweg, Erin L.; Orr, Galya; Metz, Thomas O.; Baker, Scott E.

    2015-04-23

    Yarrowia lipolytica is an oleaginous ascomycete yeast that accumulates large amounts of lipids and has potential as a biofuel producing organism. Despite a growing scientific literature focused on lipid production by Y. lipolytica, there remain significant knowledge gaps regarding the key biological processes involved. We applied a combination of metabolomic and lipidomic profiling approaches as well as microscopic techniques to identify and characterize the key pathways involved in de novo lipid accumulation from glucose in batch cultured, wild-type Y. lipolytica. We found that lipids accumulated rapidly and peaked at 48 hours during the five day experiment, concurrent with a shift in amino acid metabolism. We also report that Y. lipolytica secretes disaccharides early in batch culture and reabsorbs them when extracellular glucose is depleted. Exhaustion of extracellular sugars coincided with thickening of the cell wall, suggesting that genes involved in cell wall biogenesis may be a useful target for improving the efficiency of lipid producing yeast strains.

  3. Determining Home Range and Preferred Habitat of Feral Horses on the Nevada National Security Site Using Geographic Information Systems

    SciTech Connect (OSTI)

    Burns, Ashley V.

    2014-05-30

    Feral horses (Equus caballus) are free-roaming descendants of domesticated horses and legally protected by the Wild and Free-Roaming Horses and Burros Act of 1971, which mandates how feral horses and burros should be managed and protected on federal lands. Using a geographic information system to determine the home range and suitable habitat of feral horses on the federally managed Nevada National Security Site can enable wildlife biologists in making best management practice recommendations. Home range was estimated at 88.1 square kilometers. Site suitability was calculated for elevation, forage, slope, water presence and horse observations. These variables were combined in successive iterations into one polygon. Suitability rankings established that 85 square kilometers are most suitable habitat, with 2,052 square kilometers of good habitat 1,252 square kilometers of fair habitat and 122 square kilometers of least suitable habitat.

  4. Transposable Elements and Genetic Instabilities in Crop Plants

    DOE R&D Accomplishments [OSTI]

    Burr, B.; Burr, F.

    1981-04-10

    Transposable elements have long been associated with certain unstable loci in maize and have been intensively studied by McClintock and others. It is known that a transposable element can control the expression of the structural genes at the locus where it resides. These controlling elements in maize are now beginning to be studied at the molecular level. Using recombinant molecular probes we have been able to describe the changes induced by the controlling element Ds at the shrunken locus. Ds elements appear to be large and dissimilar insertions into the wild-type locus - two elements actually map within the transcribed region of the gene. Genetic instabilities have been described in other economically important plants but the bases for these phenomena have not been understood. We believe that it is likely that some of these instabilities are the result of transposable element activity much as in the case of maize.

  5. A novel approach to make homogeneous protease-stable monovalent streptavidin

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhang, M.; Shao, J; Xiao, J.; Deng, W.; Yu, H.

    2015-06-11

    The interaction between the tetramer streptavidin and biotin is recognized as one of the strongest non-covalent associations. Owing to the tight and specific binding, the streptavidin-biotin system has been used widely for bimolecular labeling, purification, immobilization, and even for targeted delivery of therapeutics drugs. Here, we report a novel approach to make homogeneous monovalent tetramer streptavidin. The purified monovalent protein showed both thermal stability and protease stability. Unexpectedly, we found that two proteases, Proteinase K (PK) and Subtilisin (SU), can efficiently remove the His8-tag from the wild-type subunit without affecting the tetramer architecture of monovalent streptavidin, thus making it moremore » homogeneous. In addition, crystallization was performed to assure the homogeneity of the monovalent protein prepared. Overall, monovalent streptavidin shows increased homogeneity and will likely be valuable for many future applications in a wide range of research areas.« less

  6. Effects of polychlorinated biphenyls and nutritional restriction on barbituate-induced sleeping times and selected blood characteristics in raccoons (Procyon lotor)

    SciTech Connect (OSTI)

    Montz, W.E.; Card, W.C.; Kirkpatrick, R.L.

    1982-05-01

    Hepatic microsomal enzyme activity was induced in wild-trapped raccoons (Procyon lotor) and selected blood characteristics were measured in an effort to detect responses due to PCB ingestion, nutritional restriction, and their interactions. Barbiturate-induced sleeping times were used as an index of hepatic microsomal activity because they have been used reliably by other workers. Blood characteristics examined in the study were nonesterified fatty acids (NEFA), cholesterol, and three ketone bodies (D-(-)-3-hydroxybutyrate, acetoacetate, and acetone). Results show a reduction in sleeping times, elevated NEFA and D-(-)-3-hydroxybutyrate concentrations, and lower cholesterol concentrations in PCB-treated groups. A highly significant interaction between PCB treatment and nutritional restriction was observed in acetoacetate concentrations. (JMT)

  7. Survival Estimates for the Passage of Juvenile Salmonids through Snake and Columbia River Dams and Reservoirs, 2002-2003 Annual Report.

    SciTech Connect (OSTI)

    Muir, William D.; Smith, Steven G.; Zabel, Richard W.

    2003-07-01

    In 2002, the National Marine Fisheries Service and the University of Washington completed the tenth year of a study to estimate survival and travel time of juvenile salmonids (Oncorhynchus spp.) passing through dams and reservoirs on the Snake and Columbia Rivers. All estimates were derived from detections of fish tagged with passive integrated transponder tags (PIT tags). We PIT tagged and released a total of 19,891 hatchery steelhead at Lower Granite Dam. In addition, we utilized fish PIT tagged by other agencies at traps and hatcheries upstream from the hydropower system and sites within the hydropower system. PIT-tagged smolts were detected at interrogation facilities at Lower Granite, Little Goose, Lower Monumental, McNary, John Day, and Bonneville Dams and in the PIT-tag detector trawl operated in the Columbia River estuary. Survival estimates were calculated using a statistical model for tag-recapture data from single release groups (the ''Single-Release Model''). Primary research objectives in 2002 were to (1) estimate reach and project survival and travel time in the Snake and Columbia Rivers throughout the migration period of yearling chinook salmon O. tshawytscha and steelhead O. mykiss; (2) evaluate relationships between survival estimates and migration conditions; and (3) evaluate the survival-estimation models under prevailing conditions. This report provides reach survival and travel time estimates for 2002 for PIT-tagged yearling chinook salmon (hatchery and wild), hatchery sockeye salmon O. nerka, hatchery coho salmon O. kisutch, and steelhead (hatchery and wild) in the Snake and Columbia Rivers. Results are reported primarily in the form of tables and figures; details on methodology and statistical models used are provided in previous reports cited here. Results for summer-migrating chinook salmon will be reported separately.

  8. Effect of a C298D Mutation in CaHydA [FeFe]-Hydrogenase: Insights into the Protein-Metal Cluster Interaction by EPR and FTIR Spectroscopic Investigation

    SciTech Connect (OSTI)

    Morra, Simone; Maurelli, Sara; Chiesa, Mario; Mulder, David W.; Ratzloff, Michael W.; Giamello, Elio; King, Paul W.; Gilardi, Gianfranco; Valettia, Francesca

    2016-01-01

    A conserved cysteine located in the signature motif of the catalytic center (H-cluster) of [FeFe]-hydrogenases functions in proton transfer. This residue corresponds to C298 in Clostridium acetobutylicum CaHydA. Despite the chemical and structural difference, the mutant C298D retains fast catalytic activity, while replacement with any other amino acid caused significant activity loss. Given the proximity of C298 to the H-cluster, the effect of the C298D mutation on the catalytic center was studied by continuous wave (CW) and pulse electron paramagnetic resonance (EPR) and by Fourier transform infrared (FTIR) spectroscopies. Comparison of the C298D mutant with the wild type CaHydA by CW and pulse EPR showed that the electronic structure of the center is not altered. FTIR spectroscopy confirmed that absorption peak values observed in the mutant are virtually identical to those observed in the wild type, indicating that the H-cluster is not generally affected by the mutation. Significant differences were observed only in the inhibited state Hox-CO: the vibrational modes assigned to the COexo and Fed-CO in this state are shifted to lower values in C298D, suggesting different interaction of these ligands with the protein moiety when C298 is changed to D298. More relevant to the catalytic cycle, the redox equilibrium between the Hox and Hred states is modified by the mutation, causing a prevalence of the oxidized state. This work highlights how the interactions between the protein environment and the H-cluster, a dynamic closely interconnected system, can be engineered and studied in the perspective of designing bio-inspired catalysts and mimics.

  9. Total Dissolved Gas Effects on Incubating Chum Salmon Below Bonneville Dam

    SciTech Connect (OSTI)

    Arntzen, Evan V.; Hand, Kristine D.; Carter, Kathleen M.; Geist, David R.; Murray, Katherine J.; Dawley, Earl M.; Cullinan, Valerie I.; Elston, Ralph A.; Vavrinec, John

    2009-01-29

    At the request of the U.S. Army Corps of Engineers (USACE; Portland District), Pacific Northwest National Laboratory (PNNL) undertook a project in 2006 to look further into issues of total dissolved gas (TDG) supersaturation in the lower Columbia River downstream of Bonneville Dam. In FY 2008, the third year of the project, PNNL conducted field monitoring and laboratory toxicity testing to both verify results from 2007 and answer some additional questions about how salmonid sac fry respond to elevated TDG in the field and the laboratory. For FY 2008, three objectives were 1) to repeat the 2006-2007 field effort to collect empirical data on TDG from the Ives Island and Multnomah Falls study sites; 2) to repeat the static laboratory toxicity tests on hatchery chum salmon fry to verify 2007 results and to expose wild chum salmon fry to incremental increases in TDG, above those of the static test, until external symptoms of gas bubble disease were clearly present; and 3) to assess physiological responses to TDG levels in wild chum salmon sac fry incubating below Bonneville Dam during spill operations. This report summarizes the tasks conducted and results obtained in pursuit of the three objectives. Chapter 1 discusses the field monitoring, Chapter 2 reports the findings of the laboratory toxicity tests, and Chapter 3 describes the field-sampling task. Each chapter contains an objective-specific introduction, description of the study site and methods, results of research, and discussion of findings. Literature cited throughout this report is listed in Chapter 4. Additional details on the monitoring methodology and results are provided in Appendices A and B included on the compact disc bound inside the back cover of the printed version of this report.

  10. Acetaminophen-induced acute liver injury in HCV transgenic mice

    SciTech Connect (OSTI)

    Uehara, Takeki; Kosyk, Oksana; Jeannot, Emmanuelle; Bradford, Blair U.; Tech, Katherine; Macdonald, Jeffrey M.; Boorman, Gary A.; Chatterjee, Saurabh; Mason, Ronald P.; Melnyk, Stepan B.; Tryndyak, Volodymyr P.; Pogribny, Igor P.; Rusyn, Ivan

    2013-01-15

    The exact etiology of clinical cases of acute liver failure is difficult to ascertain and it is likely that various co-morbidity factors play a role. For example, epidemiological evidence suggests that coexistent hepatitis C virus (HCV) infection increased the risk of acetaminophen-induced acute liver injury, and was associated with an increased risk of progression to acute liver failure. However, little is known about possible mechanisms of enhanced acetaminophen hepatotoxicity in HCV-infected subjects. In this study, we tested a hypothesis that HCV-Tg mice may be more susceptible to acetaminophen hepatotoxicity, and also evaluated the mechanisms of acetaminophen-induced liver damage in wild type and HCV-Tg mice expressing core, E1 and E2 proteins. Male mice were treated with a single dose of acetaminophen (300 or 500 mg/kg in fed animals; or 200 mg/kg in fasted animals; i.g.) and liver and serum endpoints were evaluated at 4 and 24 h after dosing. Our results suggest that in fed mice, liver toxicity in HCV-Tg mice is not markedly exaggerated as compared to the wild-type mice. In fasted mice, greater liver injury was observed in HCV-Tg mice. In fed mice dosed with 300 mg/kg acetaminophen, we observed that liver mitochondria in HCV-Tg mice exhibited signs of dysfunction showing the potential mechanism for increased susceptibility. -- Highlights: ? Acetaminophen-induced liver injury is a significant clinical challenge. ? HCV-infected subjects may be at higher risk for acetaminophen-induced liver injury. ? We used HCV transgenics to test if liver injury due to acetaminophen is exacerbated.

  11. Isolation and characterization of two cellulose morphology mutants of Gluconacetobacter hansenii ATCC23769 producing cellulose with lower crystallinity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Deng, Ying; Nagachar, Nivedita; Fang, Lin; Luan, Xin; Catchmark, Jeffrey M.; Tien, Ming; Kao, Teh -hui; Lai, Hsin -Chih

    2015-03-19

    Gluconacetobacter hansenii, a Gram-negative bacterium, produces and secrets highly crystalline cellulose into growth medium, and has long been used as a model system for studying cellulose synthesis in higher plants. Cellulose synthesis involves the formation of β-1,4 glucan chains via the polymerization of glucose units by a multi-enzyme cellulose synthase complex (CSC). These glucan chains assemble into ordered structures including crystalline microfibrils. AcsA is the catalytic subunit of the cellulose synthase enzymes in the CSC, and AcsC is required for the secretion of cellulose. However, little is known about other proteins required for the assembly of crystalline cellulose. To addressmore » this question, we visually examined cellulose pellicles formed in growth media of 763 individual colonies of G. hansenii generated via Tn5 transposon insertion mutagenesis, and identified 85 that produced cellulose with altered morphologies. X-ray diffraction analysis of these 85 mutants identified two that produced cellulose with significantly lower crystallinity than wild type. The gene disrupted in one of these two mutants encoded a lysine decarboxylase and that in the other encoded an alanine racemase. Solid-state NMR analysis revealed that cellulose produced by these two mutants contained increased amounts of non-crystalline cellulose and monosaccharides associated with non-cellulosic polysaccharides as compared to the wild type. Monosaccharide analysis detected higher percentages of galactose and mannose in cellulose produced by both mutants. Field emission scanning electron microscopy showed that cellulose produced by the mutants was unevenly distributed, with some regions appearing to contain deposition of non-cellulosic polysaccharides; however, the width of the ribbon was comparable to that of normal cellulose. As both lysine decarboxylase and alanine racemase are required for the integrity of peptidoglycan, we propose a model for the role of peptidoglycan in the assembly of crystalline cellulose.« less

  12. Compensatory Paracrine Mechanisms That Define The Urothelial Response to Injury in Partial Bladder Outlet Obstruction

    SciTech Connect (OSTI)

    Bassuk, James; Lendvay, Thomas S.; Sweet, Robert; Han, Chang-Hee; Soygur, Tarkan; Cheng, Jan-Fang; Plaire, J. Chadwick; Charleston, Jay S.; Charleston, Lynne B.; Bagai, Shelly; Cochrane, Kimberly; Rubio, Eric; Bassuk, James A.; Fuchs, Elaine

    2007-06-21

    Diseases and conditions affecting the lower urinary tract are a leading cause of dysfunctional sexual health, incontinence, infection, and kidney failure. The growth, differentiation, and repair of the bladder's epithelial lining are regulated, in part, by fibroblast growth factor (FGF)-7 and -10 via a paracrine cascade originating in the mesenchyme (lamina propria) and targeting the receptor for FGF-7 and -10 within the transitional epithelium (urothelium). The FGF-7 gene is located at the 15q15-q21.1 locus on chromosome 15 and four exons generate a 3.852-kb mRNA. Five duplicated FGF-7 gene sequences that localized to chromosome 9 were predicted not to generate functional protein products, thus validating the use of FGF-7-null mice as an experimental model. Recombinant FGF-7 and -10 induced proliferation of human urothelial cells in vitro and transitional epithelium of wild-type and FGF-7-null mice in vivo.To determine the extent that induction of urothelial cell proliferation during the bladder response to injury is dependent on FGF-7, an animal model of partial bladder outlet obstruction was developed. Unbiased stereology was used to measure the percentage of proliferating urothelial cells between obstructed groups of wild-type and FGF-7-null mice. The stereological analysis indicated that a statistical significant difference did not exist between the two groups, suggesting that FGF-7 is not essential for urothelial cell proliferation in response to partial outlet obstruction. In contrast, a significant increase in FGF-10 expression was observed in the obstructed FGF-7-null group, indicating that the compensatory pathway that functions in this model results in urothelial repair.

  13. Elimination of hydrogenase post-translational modification blocks H2 production and increases ethanol yield in Clostridium thermocellum

    SciTech Connect (OSTI)

    Biswas, Ranjita; Zheng, Tianyong; Olson, Daniel G.; Lynd, Lee R; Guss, Adam M

    2015-01-01

    The native ability of Clostridium thermocellum to rapidly consume cellulose and produce ethanol makes it a leading candidate for a consolidated bioprocessing (CBP) biofuel production strategy. C. thermocellum also synthesizes lactate, formate, acetate, H2, and amino acids that compete with ethanol production for carbon and electrons. Elimination of H2 production could redirect carbon flux towards ethanol production by making more electrons available for acetyl-CoA reduction to ethanol. C. thermocellum encodes four hydrogenases and rather than delete each individually, we targeted a hydrogenase maturase gene (hydG), involved in converting the three [FeFe] hydrogenase apoenzymes into holoenzymes. Further deletion of the [NiFe] hydrogenase (ech) resulted in a mutant that functionally lacks all four hydrogenases. H2 production in hydG ech was undetectable and ethanol yield increased nearly 2-fold compared to wild type. Interestingly, mutant growth improved upon the addition of acetate, which led to increased expression of genes related to sulfate metabolism, suggesting these mutants may use sulfate as a terminal electron acceptor to balance redox reactions. Genomic analysis of hydG revealed a mutation in adhE, resulting in a strain with both NADH- and NADPH-dependent alcohol dehydrogenase activities. While this same adhE mutation is found in ethanol tolerant C. thermocellum strain E50C, hydG and hydG ech are not more ethanol tolerant than wild type, illustrating the complicated interactions between redox balancing and ethanol tolerance in C. thermocellum. The dramatic increase in ethanol production here suggests that targeting protein post-translational modification is a promising new approach for inactivation of multiple enzymes simultaneously for metabolic engineering.

  14. Comparative phosphoproteomics reveals components of host cell invasion and post-transcriptional regulation during Francisella infection

    SciTech Connect (OSTI)

    Nakayasu, Ernesto S.; Tempel, Rebecca; Cambronne, Xiaolu A.; Petyuk, Vladislav A.; Jones, Marcus B.; Gritsenko, Marina A.; Monroe, Matthew E.; Yang, Feng; Smith, Richard D.; Adkins, Joshua N.; Heffron, Fred

    2013-09-22

    Francisella tularensis is a facultative intracellular bacterium that causes the deadly disease tularemia. Most evidence suggests that Francisella is not well recognized by the innate immune system that normally leads to cytokine expression and cell death. In previous work, we identified new bacterial factors that were hyper-cytotoxic to macrophages. Four of the identified hyper-cytotoxic strains (lpcC, manB, manC and kdtA) had an impaired lipopolysaccharide (LPS) synthesis and produced an exposed lipid A lacking the O-antigen. These mutants were not only hyper-cytotoxic but also were phagocytosed at much higher rates compared to the wild type parent strain. To elucidate the cellular signaling underlying this enhanced phagocytosis and cell death, we performed a large-scale comparative phosphoproteomic analysis of cells infected with wild-type and delta-lpcC F. novicida. Our data suggest that not only actin but also intermediate filaments and microtubules are important for F. novicida entry into the host cells. In addition, we observed differential phosphorylation of tristetraprolin (TTP), a key component of the mRNA-degrading machinery that controls the expression of a variety of genes including many cytokines. Infection with the delta-lpcC mutant induced the hyper-phosphorylation and inhibition of TTP, leading to the production of cytokines such as IL-1beta and TNF-alpha which may kill the host cells by triggering apoptosis. Together, our data provide new insights for Francisella invasion and a post-transcriptional mechanism that prevents the expression of host immune response factors that controls infection by this pathogen.

  15. Genetic disruption of KSHV major latent nuclear antigen LANA enhances viral lytic transcriptional program

    SciTech Connect (OSTI)

    Li Qiuhua; Zhou Fuchun; Ye Fengchun; Gao Shoujiang

    2008-09-30

    Following primary infection, KSHV establishes a lifelong persistent latent infection in the host. The mechanism of KSHV latency is not fully understood. The latent nuclear antigen (LANA or LNA) encoded by ORF73 is one of a few viral genes expressed during KSHV latency, and is consistently detected in all KSHV-related malignancies. LANA is essential for KSHV episome persistence, and regulates the expression of viral lytic genes through epigenetic silencing, and inhibition of the expression and transactivation function of the key KSHV lytic replication initiator RTA (ORF50). In this study, we used a genetic approach to examine the role of LANA in regulating KSHV lytic replication program. Deletion of LANA did not affect the expression of its adjacent genes vCyclin (ORF72) and vFLIP (ORF71). In contrast, the expression levels of viral lytic genes including immediate-early gene RTA, early genes MTA (ORF57), vIL-6 (ORF-K2) and ORF59, and late gene ORF-K8.1 were increased before and after viral lytic induction with 12-O-tetradecanoyl-phorbol-13-acetate and sodium butyrate. This enhanced expression of viral lytic genes was also observed following overexpression of RTA with or without simultaneous chemical induction. Consistent with these results, the LANA mutant cells produced more infectious virions than the wild-type virus cells did. Furthermore, genetic repair of the mutant virus reverted the phenotypes to those of wild-type virus. Together, these results have demonstrated that, in the context of viral genome, LANA contributes to KSHV latency by regulating the expression of RTA and its downstream genes.

  16. Carbonic anhydrase activity in isolated chloroplasts of chlamydomonas reinhardtii

    SciTech Connect (OSTI)

    Katzman, G.; Togasaki, R.K. ); Marcus, Y. ); Moroney, J.V. )

    1989-04-01

    In a new assay of carbonic anhydrase, NaH{sup 14}CO{sub 3} solution at the bottom of a sealed vessel releases {sup 14}CO{sub 3} which diffuses to the top of the vessel to be assimilated by actively photosynthesizing Chlamydomonas cells. The assay is initiated by illuminating cells and stopped by turning the light off and killing the cells with acid. Enzyme activity was estimated from acid stable radioactivity above the uncatalyzed background level. With bovine carbonic anhydrase, 1.5 Wilbur Anderson Unit (WAU) can be consistantly measured at 5-6 fold above background. Sonicated whole cells of air adapted wild type (+)gave 741.1 {plus minus} 12.4 WAU/mg chl. Intact washed cells of mixotrophically grown wall-less mutant CWD(-) and a high CO2 requiring wall-less double mutant CIA-3/CW15 (-) gave 7.1 {plus minus} 1.9 and 2.8 {plus minus} 7.8 WAU/mg chl respectively. Chloroplasts isolated from CWD and CIA-3/CW15 and subsequently disrupted gave 64.0 {plus minus} 14.7 and 2.8 {plus minus} 3.2 WAU/mg chl respectively. Chloroplast sonicate from another wall-less mutant CW15(-) gave activity comparable to CWD. Thus on a chlorophyll basis, enzyme activity in chloroplasts from mixotrophically grown cells is about 1/10th of the level found in air adapted wild type cells. CIA-3 seems to lack this activity.

  17. Performance and value of CAD-deficient pine- Final Report

    SciTech Connect (OSTI)

    Bailian Li; Houmin Chang; Hasan Jameel

    2007-02-28

    The southern US produces 58% of the nation's timber, much of it grown in intensively managed plantations of genetically improved loblolly pine. One of the fastest-growing loblolly pine selections made by the NCSU-Industry Cooperative Tree Improvement Program, whose progeny are widely planted, is also the only known natural carrier of a rare gene, cadn1. This allele codes for deficiency in an enzyme, cinnamyl alcohol dehydrogenase, which catalyzes the last step in the biosynthesis of lignin precursors. This study is to characterize this candidate gene for marker-assisted selection and deployment in the breeding program. This research will enhance the sustainability of forest production in the South, where land-use pressures will limit the total area available in the future for intensively managed plantations. Furthermore, this research will provide information to establish higher-value plantation forests with more desirable wood/fiber quality traits. A rare mutant allele (cad-n1) of the cad gene in loblolly pine (Pinus taeda L.) causes a deficiency in the production of cinnamyl alcohol dehydrogenase (CAD). The effects of this allele were examined by comparing wood density and growth traits of cad-n1 heterozygous trees with those of wild-type trees in a 10-year-old open-pollinated family trial growing under two levels of fertilization in Scotland County, North Carolina. In all, 200 trees were sampled with 100 trees for each treatment. Wood density measurements were collected from wood cores at breast height using x-ray densitometry. We found that the substitution of cad-n1 for a wild-type allele (Cad) was associated with a significant effect on wood density. The cad-n1 heterozygotes had a significantly higher wood density (+2.6%) compared to wild-type trees. The higher density was apparently due to the higher percentage of latewood in the heterozygotes. The fertilization effect was highly significant for both growth and wood density traits. While no cad genotype x treatment interactions was found for any of the traits studied, in the fertilized plots, the effect of the cad-n1 allele on wood density was reduced. The study indicates that the cad-n1 allele could be a valuable gene to the pulp and paper industry for the purpose of enhancing pulp yields through increasing wood density. Stem growth and wood density associated with a mutant null (cad-n1) allele were examined in three 15-year-old loblolly pine diallel tests, established on two sites in the southern United States. In each diallel test, one or two cad-n1 heterozygous parents were crossed with five unrelated wild-type parents, to produce five or ten full-sib families. In all, 839 trees from 20 full-sib families in four genetic backgrounds (a cad-n1 heterozygote x 5 unrelated trees) were sampled, genotyped at the cad locus, and assessed for growth and wood density traits. In a combined analysis of all four genetic backgrounds, we found evidence for effects of increased wood density associated with the cad-n1 allele at age 15 (p=0.03) years and height growth at ages 6 (p=0.03) and 15 (p=0.005). There were large differences in the cad-n1 effects for the various growth and wood traits among the diallel tests. This variation may be due to either different genetic backgrounds among the parents of the different diallel tests, or for different growing environments at the field sites. Even though the cad-n1 effect on growth and wood density was significant across genetic backgrounds, the effect was variable among full-sib families within backgrounds. We speculate that certain wild-type alleles from second parents specifically interact with cad-n1 producing large positive effects. In addition, pleiotropic effects on growth and wood density appear to be associated with the cad-n1 allele. While substantial gains are possible through deployment of trees carrying cad-n1, these gains may be family-specific and should be verified for each cross through field testing.

  18. Smolt Condition and Timing of Arrival at Lower Granite Reservoir, 1983-1984 Technical Report.

    SciTech Connect (OSTI)

    Scully, Richard J.; Buettner, Edwin W.

    1985-12-01

    Hatcheries released 9.3 million chinook salmon and 6.3 million steelhead smolts and presmolts upriver from Lower Granite Reservoir for migration In spring, 1984. We operated smolt monitoring traps at Whitebird from March 14 to May 12, Snake River from March 22 to May 15 and Clearwater from March 29 to May 13. Peak passage of yearling chinook salmon occurred the third week In April at both Whitebird and Snake River traps. Passage of steelhead was still increasing when high water stopped trapping in mid-May. Median migration rates for branded chinook salmon between release sites and Whitebird were 3, 17 and 15 miles/day for Rapid River, South Fork Salmon and Decker Flat smolts, respectively, an average of 11.6 miles/day. Average migration rate for these three groups between Whitebird and Snake River trap was 28 miles/day. Average migration rate between release sites and Snake River (the head of Lower Granite Reservoir) was 13.2 miles/day and from that point on through the reservoir to the dam, 1.9 miles/day. Salmon River discharge, when considered along with other environmental factors, had the greatest effect on migration rate of smolts branded both at hatcheries and at the Whitebird trap and migrating to the head of Lower Granite Reservoir. Migration rate for steelhead released from Dworshak Hatchery and recaptured at the Clearwater trap was 34 miles/day. Survival rates to the Snake River trap of branded chinook salmon smolts released at Hells Canyon Dam, Rapid River, South Fork Salmon and Decker Flat were 52%, 65%, 68% and 35%, respectively. Classical descaling, where at least 40% of the scales are missing from at least two of five areas on the side of a smolt, ranged from 0 to 5.3% at hatcheries for chinook salmon and was less than 1% for steelhead. Descaling rate often Increased about 1% at release sites. Classical descaling at Whitebird, Clearwater and Snake River traps averaged 4.5, 2.5 and 1.5% for chinook salmon, 2.1, 0.4 and 1.4% for wild steelhead and 8.7, 4.1 and 5.5% for hatchery steelhead, respectively. Scattered descaling, where at least 10% of scales are missing from at least one side of a fish, was always more extensive than was classical descaling, ranging from 2.5 times greater for Clearwater hatchery steelhead to 6.8 times greater for Clearwater wild steelhead. Mean total length of chinook salmon yearlings was the same at all the traps, i.e., 128 mm (117 mm fork length) + 1 mm. The largest chinook salmon smolts came from Dworshak National Fish Hatchery on the Clearwater River. Hatchery steelhead were smallest (2 = 203 mm) at the Clearwater trap and largest (2 = 239 mm) at the Whitebird trap. Wild steelhead were also smallest at Clearwater trap ({bar x} = 178 mm) and largest at Whitebird trap ({bar x} = 193 mm). Purse seining to evaluate rates of descaling before and after smolts passed Lower Granite Dam was largely ineffective since we were unable to catch sufficient numbers of smolts in the tailrace, and winds in the forebay area altered descaling rates in sampled smolts.

  19. Steelhead Supplementation Studies; Steelhead Supplementation in Idaho Rivers, Annual Report 2002.

    SciTech Connect (OSTI)

    Byrne, Alan

    2003-03-01

    The Steelhead Supplementation Study (SSS) has two broad objectives: (1) investigate the feasibility of supplementing depressed wild and natural steelhead populations using hatchery populations, and (2) describe the basic life history and genetic characteristics of wild and natural steelhead populations in the Salmon and Clearwater Basins. Idaho Department of Fish and Game (IDFG) personnel stocked adult steelhead from Sawtooth Fish Hatchery into Frenchman and Beaver creeks and estimated the number of age-1 parr produced from the outplants since 1993. On May 2, 2002, both Beaver and Frenchman creeks were stocked with hatchery adult steelhead. A SSS crew snorkeled the creeks in August 2002 to estimate the abundance of age-1 parr from brood year (BY) 2001. I estimated that the yield of age-1 parr per female stocked in 2001 was 7.3 and 6.7 in Beaver and Frenchman creeks, respectively. SSS crews stocked Dworshak hatchery stock fingerlings and smolts from 1993 to 1999 in the Red River drainage to assess which life stage produces more progeny when the adults return to spawn. In 2002, Clearwater Fish Hatchery personnel operated the Red River weir to trap adults that returned from these stockings. Twelve PIT-tagged adults from the smolt releases and one PIT-tagged adult from fingerling releases were detected during their migration up the mainstem Columbia and Snake rivers, but none from either group were caught at the weir. The primary focus of the study has been monitoring and collecting life history information from wild steelhead populations. An adult weir has been operated annually since 1992 in Fish Creek, a tributary of the Lochsa River. The weir was damaged by a rain-on-snow event in April 2002 and although the weir remained intact, some adults were able to swim undetected through the weir. Despite damage to the weir, trap tenders captured 167 adult steelhead, the most fish since 1993. The maximum likelihood estimate of adult steelhead escapement was 242. A screw trap has been operated annually in Fish Creek since 1994 to estimate the number of emigrating parr and smolts. I estimated that 18,687 juvenile steelhead emigrated from Fish Creek in 2002, the lowest number of migrants since 1998. SSS crews snorkeled three streams in the Selway River drainage and 10 streams in the Lochsa River drainage to estimate juvenile steelhead densities. The densities of age-1 steelhead parr declined in all streams compared to the densities observed in 2001. The age-1 densities in Fish Creek and Gedney Creek were the lowest observed since this project began monitoring those populations in 1994. The SSS crews and other cooperators tagged more than 12,000 juvenile steelhead with passive integrated transponder (PIT) tags in 2002. In 2002, technicians mounted and aged steelhead scales that were collected from 1998 to 2001. A consensus was reached among technicians for age of steelhead juveniles from Fish Creek. Scales that were collected in other streams were aged by at least one reader; however, before a final age is assigned to these fish, the age needs to be verified by another reader and any age differences among readers resolved. Dr. Jennifer Nielsen, at the U.S. Geological Survey Alaska Biological Science Center, Anchorage continued the microsatellite analysis of the steelhead tissue samples that were collected from Idaho streams in 2000. Two thousand eighteen samples from 40 populations were analyzed. The analysis of the remaining 39 populations is continuing.

  20. Enumeration of Juvenile Salmonids in the Okanogan Basin Using Rotary Screw Traps, Performance Period: March 15, 2006 - July 15, 2006.

    SciTech Connect (OSTI)

    Johnson, Peter N.; Rayton, Michael D.

    2007-05-01

    The Colville Tribes identified the need for collecting baseline census data on the timing and abundance of juvenile salmonids in the Okanogan River basin for the purpose of documenting local fish populations, augmenting existing fishery data and assessing natural production trends of salmonids. This report documents and assesses the pilot year of rotary trap capture of salmonid smolts on the Okanogan River. The project is a component of the Colville Tribes Okanogan Basin Monitoring and Evaluation Program (OBMEP) which began in 2004. Trapping for outmigrating fish began on 14 March 2006 and continued through 11 July 2006. Anadromous forms of Oncorhynchus, including summer steelhead (O. mykiss), Chinook (O. tshawytscha), and sockeye (O. nerka), were targeted for this study; all have verified, natural production in the Okanogan basin. Both 8-ft and 5-ft rotary screw traps were deployed on the Okanogan River from the Highway 20 Bridge and typically fished during evening hours or 24 hours per day, depending upon trap position and discharge conditions. Juvenile Chinook salmon were the most abundant species trapped in 2006 (10,682 fry and 2,024 smolts), followed by sockeye (205 parr and 3,291 smolts) and steelhead (1 fry and 333 smolts). Of the trapped Chinook, all fry were wild origin and all but five of the smolts were hatchery-reared. All trapped sockeye were wild origin and 88% of the steelhead smolts were hatchery-reared. Mark-recapture experiments were conducted using Chinook fry and hatchery-reared steelhead smolts (sockeye were not used in 2006 because the peak of the juvenile migration occurred prior to the onset of the mark-recapture experiments). A total of 930 chinook fry were marked and released across eight separate release dates (numbers of marked Chinook fry released per day ranged from 34 to 290 fish). A total of 11 chinook fry were recaptured for an overall trap efficiency of 1.18%. A total of 710 hatchery-reared steelhead were marked and released across three separate release dates (numbers of steelhead released per day ranged from 100 to 500 fish). A total of 12 steelhead were recaptured for an overall trap efficiency of 1.69%. A pooled Peterson estimator with a Chapman modification was used to produce population estimates for wild Chinook fry and hatchery-reared steelhead based on the results of the mark-recapture experiments. The 2006 populations for Chinook and steelhead were estimated to be 381,554 (95% confidence intervals: 175,731-587,377) and 14,164 (6,999-21,330), respectively. The population estimates were based on the periods in which mark-recapture experiments were initialized through the end of the trapping season (10 May for steelhead and 1 June for Chinook).

  1. Study on the reduction of atmospheric mercury emissions from mine waste enriched soils through native grass cover in the Mt. Amiata region of Italy

    SciTech Connect (OSTI)

    Fantozzi, L.; Dini, F.; Tamburello, L.; Pirrone, N.; Sprovieri, F.

    2013-08-15

    Atmospheric mercury emissions from mine-waste enriched soils were measured in order to compare the mercury fluxes of bare soils with those from other soils covered by native grasses. Our research was conducted near Mt. Amiata in central Italy, an area that was one of the largest and most productive mining centers in Europe up into the 1980s. To determine in situ mercury emissions, we used a Plexiglas flux chamber connected to a portable mercury analyzer (Lumex RA-915+). This allowed us to detect, in real time, the mercury vapor in the air, and to correlate this with the meteorological parameters that we examined (solar radiation, soil temperature, and humidity). The highest mercury flux values (8000 ng m{sup −2} h{sup −1}) were observed on bare soils during the hours of maximum insulation, while lower values (250 ng m{sup −2} h{sup −1}) were observed on soils covered by native grasses. Our results indicate that two main environmental variables affect mercury emission: solar radiation intensity and soil temperature. The presence of native vegetation, which can shield soil surfaces from incident light, reduced mercury emissions, a result that we attribute to a drop in the efficiency of mercury photoreduction processes rather than to decreases in soil temperature. This finding is consistent with decreases in mercury flux values down to 3500 ng m{sup −2} h{sup −1}, which occurred under cloudy conditions despite high soil temperatures. Moreover, when the soil temperature was 28 °C and the vegetation was removed from the experimental site, mercury emissions increased almost four-fold. This increase occurred almost immediately after the grasses were cut, and was approximately eight-fold after 20 h. Thus, this study demonstrates that enhancing wild vegetation cover could be an inexpensive and effective approach in fostering a natural, self-renewing reduction of mercury emissions from mercury-contaminated soils. -- Highlights: ► Mercury air/surface exchange from grass covered soil is different from bare soil. ► Light enhances mercury emissions and is the main parameter driving the process. ► The presence of wild vegetation covering the soil reduces mercury emission. ► Vegetative covers could be a solution to reduce atmospheric mercury pollution.

  2. Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Young, Jenna; Chung, Daehwan; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2014-10-09

    Background: Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. Different species vary in their abilities to degrade cellulose, and the presence of CelA, a bifunctional glycoside hydrolase that contains a Family 48 and a Family 9 catalytic domain, correlates well with cellulolytic ability in members of this genus. For example, C. hydrothermalis, which does not contain a CelA homolog, or a GH48 Family or GH9 Family glycoside hydrolase, is the least cellulolytic of the Caldicellulosiruptor species so far described. C. bescii,more » which contains CelA and expresses it constitutively, is among the most cellulolytic. In fact, CelA is the most abundant extracellular protein produced in C. bescii. The enzyme contains two catalytic units, a Family 9A-CBM3c processive endoglucanase and a Family 48 exoglucanase, joined by two Family 3b carbohydrate-binding domains. Although there are two non-reducing end-specific Family 9 and three reducing end-specific Family 48 glycoside hydrolases (producing primarily glucose and cellobiose; and cellobiose and cellotriose, respectively) in C. bescii, CelA is the only protein that combines both enzymatic activities. Results: A deletion of the celA gene resulted in a dramatic reduction in the microorganism’s ability to grow on crystalline cellulose (Avicel) and diminished growth on lignocellulosic biomass. A comparison of the overall endoglucanase and exoglucanase activities of the mutant compared with the wild-type suggests that the loss of the endoglucanase activity provided by the GH9 family domain is perhaps compensated for by other enzymes produced by the cell. In contrast, it appears that no other enzymes in the C. bescii secretome can compensate for the loss of exoglucanase activity. The change in enzymatic activity in the celA mutant resulted in a 15-fold decrease in sugar release on Avicel compared with the parent and wild-type strains. In conclusion: The exoglucanase activity of the GH48 domain of CelA plays a major role in biomass degradation within the suite of C. bescii biomass-degrading enzymes.« less

  3. Synergized resmethrin and corticosterone alter the chicken's response to west nile virus

    SciTech Connect (OSTI)

    Jankowski, Mark David; Franson, J Christian; Mostl, Erich; Porter, Warren P; Hofmeister, Erik K

    2009-01-01

    Debate concerning arbovirus control strategies remains contentious because concern regarding the relative risk of viral infection and environmental toxicant exposure is high but inadequately characterized. Taking this into account, mosquito control agencies employ aerial insecticides only after arbovirus surveillance data indicate high local mosquito-infection-rates. Successfully mitigating the risk of adult-mosquito-control insecticides ('adulticides') to non-target species such as humans, domestic animals, fish, beneficial insects and wildlife, while increasing their efficacy to reduce arbovirus outbreak intensity requires targeted scientific data from animal toxicity studies and environmental monitoring activities. Wild birds are an important reservoir host for WNv and are potentially exposed to insecticides used for mosquito control. However, no risk assessments have evaluated whether insecticides augment or extend the potential transmissibility of West Nile virus (WNv) in birds. In order to augment existing resmethrin risk assessments, we aimed to determine whether synergized resmethrin (SR) may cause chickens to develop an elevated or extended WN viremia and if subacute stress may affect its immunotoxicity. We distributed 40 chickens into four groups then exposed them prior to and during WNv infection with SR (50 {mu}g/l resmethrin + 150 {mu}g/l piperonyl butoxide) and/or 20 mg/I corticosterone (CORT) in their drinking-water. Corticosterone was given for 10 continuous days and SR was given for 3 alternate days starting the 3rd day of CORT exposure, then chickens were subcutaneously inoculated with WNv on the 5th day of CORT treatment. Compared to controls, CORT treatment extended and elevated viremia, enhanced WNv-specific antibody and increased the percentage of birds that shed oral virus, whereas SR treatment extended viremia, depressed WNv-specific IgG, and increased the percentage of CORT-treated birds that shed oral virus. Corticosterone and SR independently and interactively altered immunity to WNv in chickens. Further characterization of how variations in SR-exposure to and CORT levels in chickens and wild birds relate to laboratory WNv-infection trials is warranted in order to place these findings into an epidemiological context.

  4. Lactose-Inducible System for Metabolic Engineering of Clostridium ljungdahlii

    SciTech Connect (OSTI)

    Banerjee, A; Leang, C; Ueki, T; Nevin, KP; Lovley, DR

    2014-03-25

    The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic physiology of acetogenesis. In an attempt to expand the genetic toolbox for C. ljungdahlii, the possibility of adapting a lactose-inducible system for gene expression, previously reported for Clostridium perfringens, was investigated. The plasmid pAH2, originally developed for C. perfringens with a gusA reporter gene, functioned as an effective lactose-inducible system in C. ljungdahlii. Lactose induction of C. ljungdahlii containing pB1, in which the gene for the aldehyde/alcohol dehydrogenase AdhE1 was downstream of the lactose-inducible promoter, increased expression of adhE1 30-fold over the wild-type level, increasing ethanol production 1.5-fold, with a corresponding decrease in acetate production. Lactose-inducible expression of adhE1 in a strain in which adhE1 and the adhE1 homolog adhE2 had been deleted from the chromosome restored ethanol production to levels comparable to those in the wild-type strain. Inducing expression of adhE2 similarly failed to restore ethanol production, suggesting that adhE1 is the homolog responsible for ethanol production. Lactose-inducible expression of the four heterologous genes necessary to convert acetyl coenzyme A (acetyl-CoA) to acetone diverted ca. 60% of carbon flow to acetone production during growth on fructose, and 25% of carbon flow went to acetone when carbon monoxide was the electron donor. These studies demonstrate that the lactose-inducible system described here will be useful for redirecting carbon and electron flow for the biosynthesis of products more valuable than acetate. Furthermore, this tool should aid in optimizing microbial electrosynthesis and for basic studies on the physiology of acetogenesis.

  5. Active site proton delivery and the lyase activity of human CYP17A1

    SciTech Connect (OSTI)

    Khatri, Yogan; Gregory, Michael C.; Grinkova, Yelena V.; Denisov, Ilia G.; Sligar, Stephen G.

    2014-01-03

    Highlights: The disruption of PREG/PROG hydroxylation activity by T306A showed the participation of Cpd I. T306A supports the involvement of a nucleophilic peroxo-anion during lyase activity. The presence of cytochrome b{sub 5} augments CC lyase activity. ?5-Steroids are preferred substrates for CYP17 catalysis. -- Abstract: Cytochrome P450 CYP17A1 catalyzes a series of reactions that lie at the intersection of corticoid and androgen biosynthesis and thus occupies an essential role in steroid hormone metabolism. This multifunctional enzyme catalyzes the 17?-hydroxylation of ?4- and ?5-steroids progesterone and pregnenolone to form the corresponding 17?-hydroxy products through its hydroxylase activity, and a subsequent 17,20-carboncarbon scission of pregnene-side chain produce the androgens androstenedione (AD) and dehydroepiandrosterone (DHEA). While the former hydroxylation reaction is believed to proceed through a conventional Compound I rebound mechanism, it has been suggested that the latter carbon cleavage is initiated by an iron-peroxy intermediate. We report on the role of Thr306 in CYP17 catalysis. Thr306 is a member of the conserved acid/alcohol pair thought to be essential for the efficient delivery of protons required for hydroperoxoanion heterolysis and formation of Compound I in the cytochromes P450. Wild type and T306A CYP17A1 self-assembled in Nanodiscs were used to quantitate turnover and coupling efficiencies of CYP17s physiological ?4- and ?5-substrates. We observed that T306A co-incorporated in Nanodiscs with its redox partner cytochrome P450 oxidoreductase, coupled NADPH only by 0.9% and 0.7% compared to the wild type (97% and 22%) during the conversion of pregnenolone and progesterone, respectively, to the corresponding 17-OH products. Despite increased oxidation of pyridine nucleotide, hydroxylase activity was drastically diminished in the T306A mutant, suggesting a high degree of uncoupling in which reducing equivalents and protons are funneled into non-productive pathways. This is similar to previous work with other P450 catalyzed hydroxylation. However, catalysis of carboncarbon bond scission by the T306A mutant was largely unimpeded by disruption of the CYP17A1 acid-alcohol pair. The unique response of CYP17A1 lyase activity to mutation of Thr306 is consistent with a reactive intermediate formed independently of proton delivery in the active site, and supports involvement of a nucleophilic peroxo-anion rather than the traditional Compound I in catalysis.

  6. Reproductive biomarkers responses induced by xenoestrogens in the characid fish Astyanax fasciatus inhabiting a South American reservoir: An integrated field and laboratory approach

    SciTech Connect (OSTI)

    Prado, Paula S.; Pinheiro, Ana Paula B.; Bazzoli, Nilo; Rizzo, Elizete

    2014-05-01

    Field studies evaluating the effects of endocrine disruption chemicals (EDCs) on the fish reproduction are scarce worldwide. The goal of this study was to assess hepatic levels of vitellogenin (Vtg), zona radiata proteins (Zrp) and insulin-like growth factors (IGF-I and IGF-II), and relating them to reproductive endpoints in a wild fish population habiting a reservoir that receive domestic sewage, agricultural and industrial residues. Adult fish Astyanax fasciatus were sampled during the reproductive season in five sites from the Furnas Reservoir, Grande River, and ParaguayParan basin. As a control to field data, fish were experimentally exposed via dietary intake, to oestradiol benzoate (OB) for 7 days. Fish from site with little anthropogenic interference showed hepatic levels of Vtg, Zrp and IGF-I and IGF-II similar to those from the non-treated experimental group. In sites located immediately downstream from the municipal wastewater discharges, the water total oestrogen was >120 ng/l, and male fish displayed increased Vtg and Zrp and decreased IGF-I levels similar to OB treated fish. In females, levels of Vtg, Zrp, IGF-I and IGF-II suggest an impairment of final oocyte maturation and spawning, as also detected by frequency of over-ripening, follicular atresia and fecundity. At the sites that receive agricultural and industrial residues, the water total oestrogen was <50 ng/l and females showed decreased Zrp and increased IGF-II levels associated to reduced diameter of vitellogenic follicles, indicating an inhibition of oocyte growth. Overall, the current study reports oestrogenic contamination impairing the reproduction of a wild fish from a hydroeletric reservoir and, the data contribute to improving the current knowledge on relationship between hepatic Vtg, Zrp and IGF-I and IGF-II, and reproductive endpoints in a teleost fish. In addition, our data point out novel reproductive biomarkers (IGF-I, IGF-II and over-ripening) to assessing xenoestrogenic contamination in freshwater ecosystems. - Highlights: We point out novel reproductive biomarkers to assess xenoestrogenic contamination. Field captured fish showed altered hepatic Vtg and Zrp. Hepatic IGF-I and II levels were associated to reproductive disturbances. Over-ripening is a better xenoestrogen biomarker than follicular atresia.

  7. Detection of genotoxic and non-genotoxic carcinogens in Xpc{sup ?/?}p53{sup +/?} mice

    SciTech Connect (OSTI)

    Melis, Joost P.M.; Leiden University Medical Center, Department of Toxicogenetics, Leiden ; Speksnijder, Ewoud N.; Kuiper, Raoul V.; Dutch Molecular Pathology Center, Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht ; Salvatori, Daniela C.F.; Schaap, Mirjam M.; Leiden University Medical Center, Department of Toxicogenetics, Leiden ; Maas, Saskia; Robinson, Joke; Verhoef, Aart; Benthem, Jan van; Luijten, Mirjam; Steeg, Harry van

    2013-01-15

    An accurate assessment of the carcinogenic potential of chemicals and pharmaceutical drugs is essential to protect humans and the environment. Therefore, substances are extensively tested before they are marketed to the public. Currently, the rodent two-year bioassay is still routinely used to assess the carcinogenic potential of substances. However, over time it has become clear that this assay yields false positive results and also has several economic and ethical drawbacks including the use of large numbers of animals, the long duration, and the high cost. The need for a suitable alternative assay is therefore high. Previously, we have proposed the Xpa*p53 mouse model as a very suitable alternative to the two-year bioassay. We now show that the Xpc*p53 mouse model preserves all the beneficial traits of the Xpa*p53 model for sub-chronic carcinogen identification and can identify both genotoxic and non-genotoxic carcinogens. Moreover, Xpc*p53 mice appear to be more responsive than Xpa*p53 mice towards several genotoxic and non-genotoxic carcinogens. Furthermore, Xpc*p53 mice are far less sensitive than Xpa*p53 mice for the toxic activity of DNA damaging agents and as such clearly respond in a similar way as wild type mice do. These advantageous traits of the Xpc*p53 model make it a better alternative for in vivo carcinogen testing than Xpa*p53. This pilot study suggests that Xpc*p53 mice are suited for routine sub-chronic testing of both genotoxic and non-genotoxic carcinogens and as such represent a suitable alternative to possibly replace the murine life time cancer bioassay. Highlights: ? The Xpc*p53 mouse model is able to identify genotoxic and non-genotoxic carcinogens. ? Time, animals and cost can be significantly reduced compared to the 2-year bioassay. ? Xpc*p53 mice are more advantageous for carcinogen identification than Xpa*p53 mice. ? Xpc*p53 mice exhibit a wild type response upon exposure to genotoxicants.

  8. Plant nitrogen regulatory P-PII polypeptides

    DOE Patents [OSTI]

    Coruzzi, Gloria M.; Lam, Hon-Ming; Hsieh, Ming-Hsiun

    2004-11-23

    The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the invention may be used to engineer organisms to overexpress wild-type or mutant P-PII regulatory protein. Engineered plants that overexpress or underexpress P-PII regulatory protein may have increased nitrogen assimilation capacity. Engineered organisms may be used to produce P-PII proteins which, in turn, can be used for a variety of purposes including in vitro screening of herbicides. P-PII nucleotide sequences have additional uses as probes for isolating additional genomic clones having the promoters of P-PII gene. P-PII promoters are light- and/or sucrose-inducible and may be advantageously used in genetic engineering of plants.

  9. Post-Release Attributes and Survival of Hatchery and Natural Fall Chinook Salmon in the Snake River : Annual Report 1999.

    SciTech Connect (OSTI)

    Tiffan, Kenneth F.; Rondorf, Dennis W.

    2001-01-01

    This report summarizes results of research activities conducted in 1999 and years previous. In an effort to provide this information to a wider audience, the individual chapters in this report have been submitted as manuscripts to peer-reviewed journals. These chapters communicate significant findings that will aid in the management and recovery of fall chinook salmon in the Columbia River Basin. Abundance and timing of seaward migration of Snake River fall chinook salmon was indexed using passage data collected at Lower Granite Dam for five years. We used genetic analyses to determine the lineage of fish recaptured at Lower Granite Dam that had been previously PIT tagged. We then used discriminant analysis to determine run membership of PIT-tagged smolts that were not recaptured to enable us to calculate annual run composition and to compared early life history attributes of wild subyearling fall and spring chinook salmon. Because spring chinook salmon made up from 15.1 to 44.4% of the tagged subyearling smolts that were detected passing Lower Granite Dam, subyearling passage data at Lower Granite Dam can only be used to index fall chinook salmon smolt abundance and passage timing if genetic samples are taken to identify run membership of smolts. Otherwise, fall chinook salmon smolt abundance would be overestimated and timing of fall chinook salmon smolt passage would appear to be earlier and more protracted than is the case.

  10. An Insertion Mutation That Distorts Antibody Binding Site Architecture Enhances Function of a Human Antibody

    SciTech Connect (OSTI)

    Krause, Jens C.; Ekiert, Damian C.; Tumpey, Terrence M.; Smith, Patricia B.; Wilson, Ian A.; Crowe, Jr., James E.

    2011-09-02

    The structural and functional significance of somatic insertions and deletions in antibody chains is unclear. Here, we demonstrate that a naturally occurring three-amino-acid insertion within the influenza virus-specific human monoclonal antibody 2D1 heavy-chain variable region reconfigures the antibody-combining site and contributes to its high potency against the 1918 and 2009 pandemic H1N1 influenza viruses. The insertion arose through a series of events, including a somatic point mutation in a predicted hot-spot motif, introduction of a new hot-spot motif, a molecular duplication due to polymerase slippage, a deletion due to misalignment, and additional somatic point mutations. Atomic resolution structures of the wild-type antibody and a variant in which the insertion was removed revealed that the three-amino-acid insertion near the base of heavy-chain complementarity-determining region (CDR) H2 resulted in a bulge in that loop. This enlarged CDR H2 loop impinges on adjacent regions, causing distortion of the CDR H1 architecture and its displacement away from the antigen-combining site. Removal of the insertion restores the canonical structure of CDR H1 and CDR H2, but binding, neutralization activity, and in vivo activity were reduced markedly because of steric conflict of CDR H1 with the hemagglutinin antigen.

  11. Geobacter strains that use alternate organic compounds, methods of making, and methods of use thereof

    DOE Patents [OSTI]

    Lovley, Derek R; Summers, Zarath Morgan; Haveman, Shelley Annette; Izallalen, Mounir

    2013-12-03

    In preferred embodiments, the present invention provides new isolated strains of Geobacter species that are capable of using a carbon source that is selected from C.sub.3 to C.sub.12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. In other aspects, other preferred embodiments of the present invention include methods of making such strains and methods of using such strains. In general, the wild type strain of the microorganisms has been shown to be unable to use these C.sub.3 to C.sub.12 organic compounds as electron donors in metabolic steps such as the reduction of metallic ions. The inventive strains of microorganisms are useful improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, power generation from small and large-scale waste facilities (e.g., biomass waste from dairy, agriculture, food processing, brewery, or vintner industries, etc.) using microbial fuel cells, and other applications of microbial fuel cells, including, but not limited to, improved electrical power supplies for environmental sensors, electronic sensors, and electric vehicles.

  12. Geobacter strains that use alternate organic compounds, methods of making, and methods of use thereof

    DOE Patents [OSTI]

    Lovley, Derek R.; Summers, Zarath Morgan; Haveman, Shelley Annette; Izallalen, Mounir

    2016-03-01

    In preferred embodiments, the present invention provides new isolated strains of a Geobacter species that are capable of using a carbon source that is selected from C.sub.3 to C.sub.12 organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. The wild type strain of the microorganisms has been shown to be unable to use these C.sub.3 to C.sub.12 organic compounds as electron donors. The inventive strains of microorganisms are useful for improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, power generation from small and large-scale waste facilities (e.g., biomass waste from dairy, agriculture, food processing, brewery, or vintner industries, etc.) using microbial fuel cells, and other applications of microbial fuel cells, including, but not limited to, improved electrical power supplies for environmental sensors, electronic devices, and electric vehicles.

  13. Crystal structure of the FLT3 kinase domain bound to the inhibitor quizartinib (AC220)

    SciTech Connect (OSTI)

    Zorn, Julie A.; Wang, Qi; Fujimura, Eric; Barros, Tiago; Kuriyan, John; Boggon, Titus J.

    2015-04-02

    More than 30% of acute myeloid leukemia (AML) patients possess activating mutations in the receptor tyrosine kinase FMS-like tyrosine kinase 3 or FLT3. A small-molecule inhibitor of FLT3 (known as quizartinib or AC220) that is currently in clinical trials appears promising for the treatment of AML. Here, we report the co-crystal structure of the kinase domain of FLT3 in complex with quizartinib. FLT3 with quizartinib bound adopts an “Abl-like” inactive conformation with the activation loop stabilized in the “DFG-out” orientation and folded back onto the kinase domain. This conformation is similar to that observed for the uncomplexed intracellular domain of FLT3 as well as for related receptor tyrosine kinases, except for a localized induced fit in the activation loop. The co-crystal structure reveals the interactions between quizartinib and the active site of FLT3 that are key for achieving its high potency against both wild-type FLT3 as well as a FLT3 variant observed in many AML patients. This co-complex further provides a structural rationale for quizartinib-resistance mutations.

  14. Scientific Data Management (SDM) Center for Enabling Technologies. 2007-2012

    SciTech Connect (OSTI)

    Ludascher, Bertram; Altintas, Ilkay

    2013-09-06

    Over the past five years, our activities have both established Kepler as a viable scientific workflow environment and demonstrated its value across multiple science applications. We have published numerous peer-reviewed papers on the technologies highlighted in this short paper and have given Kepler tutorials at SC06,SC07,SC08,and SciDAC 2007. Our outreach activities have allowed scientists to learn best practices and better utilize Kepler to address their individual workflow problems. Our contributions to advancing the state-of-the-art in scientific workflows have focused on the following areas. Progress in each of these areas is described in subsequent sections. Workflow development. The development of a deeper understanding of scientific workflows "in the wild" and of the requirements for support tools that allow easy construction of complex scientific workflows; Generic workflow components and templates. The development of generic actors (i.e.workflow components and processes) which can be broadly applied to scientific problems; Provenance collection and analysis. The design of a flexible provenance collection and analysis infrastructure within the workflow environment; and, Workflow reliability and fault tolerance. The improvement of the reliability and fault-tolerance of workflow environments.

  15. Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Thomas, E. V.; Phillippy, K. H.; Brahamsha, B.; Haaland, D. M.; Timlin, J. A.; Elbourne, L. D. H.; Palenik, B.; Paulsen, I. T.

    2009-01-01

    Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array. A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium. Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology. Due in partmore » to the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains. One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition.« less

  16. Savannah River Ecology Laboratory. Annual technical progress report of ecological research, period ending July 31, 1994

    SciTech Connect (OSTI)

    Not Available

    1994-07-31

    The Savannah River Ecology Laboratory (SREL) is a research unit of the University of Georgia (UGA) that is managed in conjunction with the University`s Institute of Ecology. The laboratory`s overall mission is to acquire and communicate knowledge of ecological processes and principles. SREL conducts basic and applied ecological research, as well as education and outreach programs, under an M&O contract with the US Department of Energy at the Savannah River Site. Significant accomplishments were made during the year ending July 31, 1994 in the areas of research, education and service. Reviewed in this document are research projects in the following areas: Environmental Operations Support (impacted wetlands, streams, trace organics, radioecology, database synthesis, wild life studies, zooplankton, safety and quality assurance); wood stork foraging and breeding ecology; defence waste processing facility; environmental risk assessment (endangered species, fish, ash basin studies); ecosystem alteration by chemical pollutants; wetlands systems; biodiversity on the SRS; Environmental toxicology; environmental outreach and education; Par Pond drawdown studies in wildlife and fish and metals; theoretical ecology; DOE-SR National Environmental Research Park; wildlife studies. Summaries of educational programs and publications are also give.

  17. Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. [Salmonella typhimurium; Escherichia coli; Sarcina lutea; Staphylococcus aureus; Streptococcus lactis; Streptococcus faecalis

    SciTech Connect (OSTI)

    Dahl, T.A.; Midden, W.R. ); Hartman, P.E. )

    1989-04-01

    Gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. Killing curves for Salmonella typhimurium and Escherichia coli strains were indicative of multihit killing, whereas curves for Sarcina lutea, Staphylococcus aureus, Streptococcus lactis, and Streptococcus faecalis exhibited single-hit kinetics. The S. typhimurium deep rough strain TA1975, which lacks nearly all of the cell wall lipopolysaccharide coat and manifests concomitant enhancement of penetration by some exogenous substances, responded to singlet oxygen with initially faster inactivation than did the S. typhimurium wild-type strain, although the maximum rates of killing appeared to be quite similar. The structure of the cell wall thus plays an important role in susceptibility to singlet oxygen. The outer membrane-lipopolysaccharide portion of the gram-negative cell wall initially protects the bacteria from extracellular singlet oxygen, although it may also serve as a source for secondary reaction products which accentuate the rates of cell killing. S. typhimurium and E. coli strains lacking the cellular antioxidant, glutathione, showed no difference from strains containing glutathione in response to the toxic effects of singlet oxygen. Strains of Sarcina lutea and Staphylococcus aureus that contained carotenoids, however, were far more resistant to singlet oxygen lethality than were both carotenoidless mutants of the same species and other gram-positive species lacking high levels of protective carotenoids.

  18. Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance

    SciTech Connect (OSTI)

    Jia, Fengjuan Qi, Shengdong Li, Hui Liu, Pu Li, Pengcheng Wu, Changai Zheng, Chengchao Huang, Jinguang

    2014-11-28

    Highlights: It is the first time to investigate the biological function of AtLEA14 in salt stress response. AtLEA14 enhances the salt stress tolerance both in Arabidopsis and yeast. AtLEA14 responses to salt stress by stabilizing AtPP2-B11, an E3 ligase, under normal or salt stress conditions. - Abstract: Late embryogenesis abundant (LEA) proteins are implicated in various abiotic stresses in higher plants. In this study, we identified a LEA protein from Arabidopsis thaliana, AtLEA14, which was ubiquitously expressed in different tissues and remarkably induced with increased duration of salt treatment. Subcellular distribution analysis demonstrated that AtLEA14 was mainly localized in the cytoplasm. Transgenic Arabidopsis and yeast overexpressing AtLEA14 all exhibited enhanced tolerance to high salinity. The transcripts of salt stress-responsive marker genes (COR15a, KIN1, RD29B and ERD10) were overactivated in AtLEA14 overexpressing lines compared with those in wild type plants under normal or salt stress conditions. In vivo and in vitro analysis showed that AtLEA14 could effectively stabilize AtPP2-B11, an important E3 ligase. These results suggested that AtLEA14 had important protective functions under salt stress conditions in Arabidopsis.

  19. Molecular crowding overcomes the destabilizing effects of mutations in a bacterial ribozyme

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lee, Hui-Ting; Kilburn, D.; Behrouzi, R.; Briber, R. M.; Woodson, S. A.

    2014-12-05

    The native structure of the Azoarcus group I ribozyme is stabilized by the cooperative formation of tertiary interactions between double helical domains. Thus, even single mutations that break this network of tertiary interactions reduce ribozyme activity in physiological Mg2+ concentrations. Here, we report that molecular crowding comparable to that in the cell compensates for destabilizing mutations in the Azoarcus ribozyme. Small angle X-ray scattering, native polyacrylamide gel electrophoresis and activity assays were used to compare folding free energies in dilute and crowded solutions containing 18% PEG1000. Crowder molecules allowed the wild-type and mutant ribozymes to fold at similarly low Mg2+more » concentrations and stabilized the active structure of the mutant ribozymes under physiological conditions. This compensation helps explains why ribozyme mutations are often less deleterious in the cell than in the test tube. Nevertheless, crowding did not rescue the high fraction of folded but less active structures formed by double and triple mutants. We conclude that crowding broadens the fitness landscape by stabilizing compact RNA structures without improving the specificity of self-assembly.« less

  20. P1-Substituted Symmetry-Based Human Immunodeficiency Virus Protease Inhibitors with Potent Antiviral Activity against Drug-Resistant Viruses

    SciTech Connect (OSTI)

    DeGoey, David A.; Grampovnik, David J.; Chen, Hui-Ju; Flosi, William J.; Klein, Larry L.; Dekhtyar, Tatyana; Stoll, Vincent; Mamo, Mulugeta; Molla, Akhteruzzaman; Kempf, Dale J.

    2013-03-07

    Because there is currently no cure for HIV infection, patients must remain on long-term drug therapy, leading to concerns over potential drug side effects and the emergence of drug resistance. For this reason, new and safe antiretroviral agents with improved potency against drug-resistant strains of HIV are needed. A series of HIV protease inhibitors (PIs) with potent activity against both wild-type (WT) virus and drug-resistant strains of HIV was designed and synthesized. The incorporation of substituents with hydrogen bond donor and acceptor groups at the P1 position of our symmetry-based inhibitor series resulted in significant potency improvements against the resistant mutants. By this approach, several compounds, such as 13, 24, and 29, were identified that demonstrated similar or improved potencies compared to 1 against highly mutated strains of HIV derived from patients who previously failed HIV PI therapy. Overall, compound 13 demonstrated the best balance of potency against drug resistant strains of HIV and oral bioavailability in pharmacokinetic studies. X-ray analysis of an HIV PI with an improved resistance profile bound to WT HIV protease is also reported.

  1. Down-regulation of the Caffeic acid O-methyltransferase Gene in Switchgrass Reveals a Novel Monolignol Analog

    SciTech Connect (OSTI)

    Tschaplinski, Timothy J; Standaert, Robert F; Engle, Nancy L; Martin, Madhavi Z; Sangha, Amandeep K; Parks, Jerry M; Smith, Jeremy C; Samuel, Reichel; Pu, Yunqiao; Ragauskas, A J; Hamilton, Choo Yieng; Fu, Chunxiang; Wang, Zeng-Yu; Davison, Brian H; Dixon, Richard A; Mielenz, Jonathan R

    2012-01-01

    Down-regulation of the caffeic acid 3-O-methyltransferase (COMT) gene in the lignin biosynthetic pathway of switchgrass (Panicum virgatum) resulted in cell walls of transgenic plants releasing more constituent sugars after pretreatment by dilute acid and treatment with glycosyl hydrolases from an added enzyme preparation and from Clostridium thermocellum. Fermentation of both wild-type and transgenic switchgrass after milder hot water pretreatment with no water washing showed that only the transgenic switchgrass inhibited C. thermocellum. Gas chromatography-mass spectrometry-based metabolomics were undertaken on cell wall aqueous extracts to determine the nature of the microbial inhibitors, confirming the increased concentration of a number of phenolic acids and aldehydes that are known inhibitors of fermentation. Metabolomic analyses of the transgenic biomass additionally revealed the presence of a novel monolignol-like metabolite, identified as trans-3, 4-dimethoxy-5-hydroxycinnamyl alcohol (iso-sinapyl alcohol) in both non-pretreated, as well as hot water pretreated samples. Although there was no indication that iso-sinapyl alcohol was integrated into the cell wall, diversion of substrates from sinapyl alcohol to free iso-sinapyl alcohol, its glucoside, and associated upstream lignin pathway changes, including increased phenolic aldehydes and acids, are associated with more facile cell wall deconstruction, and to the observed inhibitory effect on microbial growth.

  2. Cell fate regulation governed by a repurposed bacterial histidine kinase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Childers, W. Seth; Xu, Qingping; Mann, Thomas H.; Mathews, Irimpan I.; Blair, Jimmy A.; Deacon, Ashley M.; Shapiro, Lucy; Stock, Ann M.

    2014-10-28

    One of the simplest organisms to divide asymmetrically is the bacterium Caulobacter crescentus. The DivL pseudo-histidine kinase, positioned at one cell pole, regulates cell-fate by controlling the activation of the global transcription factor CtrA via an interaction with the response regulator (RR) DivK. DivL uniquely contains a tyrosine at the histidine phosphorylation site, and can achieve these regulatory functions in vivo without kinase activity. Determination of the DivL crystal structure and biochemical analysis of wild-type and site-specific DivL mutants revealed that the DivL PAS domains regulate binding specificity for DivK~P over DivK, which is modulated by an allosteric intramolecular interactionmore » between adjacent domains. We discovered that DivL's catalytic domains have been repurposed as a phosphospecific RR input sensor, thereby reversing the flow of information observed in conventional histidine kinase (HK)-RR systems and coupling a complex network of signaling proteins for cell-fate regulation.« less

  3. Seasonal changes in dissolved-gas supersaturation in the Sacramento River and possible effects on striped bass

    SciTech Connect (OSTI)

    Colt, J.

    1984-09-01

    Dissolved-gad supersaturation levels were monitored in the Sacramento River system in central California during 1981-1982. Gas supersaturation was highest in the spring when temperature and flow were increasing rapidly, and was caused primarily by inflows of highly supersaturated water from the American and Feather rivers. During high runoff, air entrained by falls and rapids can produce supersaturation. Rapid heating can produce gas supersaturation because the solubility of gases is reduced at higher temperatures. Entrainment of air at dams does not appear to be responsible for gas supersaturation in these two rivers, although the dams may have an influence on dissolved gas levels in the Sacramento River. Gas supersaturation in the Sacramento-San Joaquin River system may adversely affect the eggs and larvae of wild striped bass Morone saxatilis and salmonids in hatcheries. The siting of salmonid hatcheries below large dams insures that hatchery fish will be exposed to high levels of gas supersaturation. Because larval striped bass are positively phototactic, they are at greater risk than fish that are found lower in the water column. 48 references, 4 figures, 3 tables.

  4. Optimal Conventional and Semi-Natural Treatments for the Upper Yakima Spring Chinook Salmon Supplementation Project; Treatment Definitions and Descriptions and Biological Specifications for Facility Design, 1995-1999 Final Report.

    SciTech Connect (OSTI)

    Hager, Robert C.; Costello, Ronald J.

    1999-10-01

    This report describes the Yakima Fisheries Project facilities (Cle Elum Hatchery and acclimation satellites) which provide the mechanism to conduct state-of-the-art research for addressing questions about spring chinook supplementation strategies. The definition, descriptions, and specifications for the Yakima spring chinook supplementation program permit evaluation of alternative fish culture techniques that should yield improved methods and procedures to produce wild-like fish with higher survival that can be used to rebuild depleted spring chinook stocks of the Columbia River Basin. The definition and description of three experimental treatments, Optimal Conventional (OCT), Semi-Natural (SNT), Limited Semi-Natural (LSNT), and the biological specifications for facilities have been completed for the upper Yakima spring chinook salmon stock of the Yakima Fisheries Project. The task was performed by the Biological Specifications Work Group (BSWG) represented by Yakama Indian Nation, Washington Department of Fish and Wildlife, National Marine Fisheries Service, and Bonneville Power Administration. The control and experimental variables of the experimental treatments (OCT, SNT, and LSNT) are described in sufficient detail to assure that the fish culture facilities will be designed and operated as a production scale laboratory to produce and test supplemented upper Yakima spring chinook salmon. Product specifications of the treatment groups are proposed to serve as the generic templates for developing greater specificity for measurements of product attributes. These product specifications will be used to monitor and evaluate treatment effects, with respect to the biological response variables (post release survival, long-term fitness, reproductive success and ecological interactions).

  5. Willamette Oxygen Supplementation Studies : Annual Report 1994.

    SciTech Connect (OSTI)

    Ewing, R.D.; Ewing, S.K.; Sheahan, J.E.

    1994-09-01

    Hydropower development and operations in the Columbia River basin have caused the loss of 5 million to 11 million salmonids. An interim goal of the Northwest Power Planning Council is to reestablish these historical numbers by doubling the present runs from 2.5 million adult fish to 5.0 million adult fish. This increase in production will be accomplished through comprehensive management of both wild and hatchery fish, but artificial propagation will play a major role in the augmentation process. The current husbandry techniques in existing hatcheries require improvements that may include changes in rearing densities, addition of oxygen, removal of excess nitrogen, and improvement in raceway design. Emphasis will be placed on the ability to increase the number of fish released from hatcheries that survive to return as adults. Rearing density is one of the most important elements in fish culture. Fish culturists have attempted to rear fish in hatchery ponds at densities that most efficiently use the rearing space available. Such efficiency studies require a knowledge of cost of rearing and the return of adults to the fisheries and to the hatchery.

  6. Development of a Natural Rearing System to Improve Supplemental Fish Quality, 1991-1995 Progress Report.

    SciTech Connect (OSTI)

    Maynard, Desmond J.; Flagg, Thomas A.; Mahnken, Conrad V.W.

    1996-08-01

    In this report, the National Marine Fisheries Service (NMFS), in collaboration with the Bonneville Power Administration (BPA), the Washington State Department of Fish and Wildlife (WDFW), and the US Fish and Wildlife Service (USFWS), presents research findings and guidelines for development and evaluation of innovative culture techniques to increase postrelease survival of hatchery fish. The Natural Rearing Enhancement System (NATURES) described in this report is a collection of experimental approaches designed to produce hatchery-reared chinook salmon (Oncorhynchus tshawytscha) that exhibit wild-like behavior, physiology, and morphology. The NATURES culture research for salmonids included multiple tests to develop techniques such as: raceways equipped with cover, structure, and natural substrates to promote development of proper body camouflage coloration; feed-delivery systems that condition fish to orient to the bottom rather than the surface of the rearing vessel; predator conditioning of fish to train them to avoid predators; and supplementing diets with natural live foods to improve foraging ability. The underlying assumptions are that NATURES will: (1) promote the development of natural cryptic coloration and antipredator behavior; (2) increase postrelease foraging efficiency; (3) improve fish health and condition by alleviating chronic, artificial rearing habitat-induced stress; and (4) reduce potential genetic selection pressures induced by the conventional salmon culture environment. A goal in using NATURES is to provide quality fish for rebuilding depleted natural runs.

  7. Prevention of Prespawning Mortality: Cause of Salmon Headburns and Cranial Lesions

    SciTech Connect (OSTI)

    Neitzel, Duane A.; Elston, R A.; Abernethy, Cary S.

    2004-06-01

    This project was to undertaken to provide information about a condition known as ''headburn''. Information from the project will enable U.S. Corps of Engineers managers to make adjustments in operational procedures or facilities on the Columbia and Snake rivers to prevent loss of pre-spawning adult salmonids that migrate through the facilities. Headburn is a descriptive clinical term used by fishery biologists to describe scalping or exfoliation of skin and ulceration of underlying connective tissue and muscle, primarily of the jaw and cranial region of salmonids observed at fish passage facilities. Headburn lesions are primarily caused when fish collide with concrete or other structures at dams and fish passage facilities, and may be exacerbated in some fish that ''fallback'' or pass over spillways or through turbine assemblies after having passed the dam through a fish ladder. Prespawning mortality of headburned salmonids can be prevented or greatly reduced by therapeutic treatment of both hatchery and wild fish. Treatments would consist of topical application of an anti-fungal agent, injection of replacement plasma electrolytes into the peritoneal cavity, and injection of a broad-spectrum antibacterial agent at fish passage and trapping facilities or hatcheries.

  8. Statistical Analysis of Microarray Data with Replicated Spots: A Case Study withSynechococcusWH8102

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Thomas, E. V.; Phillippy, K. H.; Brahamsha, B.; Haaland, D. M.; Timlin, J. A.; Elbourne, L. D. H.; Palenik, B.; Paulsen, I. T.

    2009-01-01

    Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array. A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium. Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology. Due in partmoreto the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains. One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition.less

  9. The iojap gene in maize

    SciTech Connect (OSTI)

    Martienssen, Robert

    2001-12-01

    The classical maize mutant iojap (Iodent japonica) has variegated green and white leaves. Green sectors have cells with normal chloroplasts whereas white sectors have cells where plastids fail to differentiate. These mutant plastids, when transmitted through the female gametophyte, do not recover in the presence of wild type Iojap. We cloned the Ij locus, and we have investigated the mechanism of epigenetic inheritance and phenotypic expression. More recently, a modifier of this type of variegation, ''Inhibitor of striate'', has also been cloned. Both the iojap and inhibitor of striate proteins have homologs in bacteria and are members of ancient conserved families found in multiple species. These tools can be used to address fundamental questions of inheritance and variegation associated with this classical conundrum of maize genetics. Since the work of Rhoades there has been considerable speculation concerning the nature of the Iojap gene product, the origin of leaf variegation and the mechanism behind the material inheritance of defective plastids. This has made Iojap a textbook paradigm for cytoplasmic inheritance and nuclear-organellar interaction for almost 50 years. Cloning of the Iojap gene in maize, and homologs in other plants and bacteria, provides a new means to address the origin of heteroplastidity, variegation and cytoplasmic inheritance in higher plants.

  10. A limited role for carbonic anhydrase in C4 photosynthesis as revealed by a ca1ca2 double mutant in maize.

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Studer, Anthony J.; Gandin, Anthony; Kolbe, Allison R.; Wang, Lin; Cousins, Asaph B.; Brutnell, Thomas P.

    2014-04-04

    Carbonic anhydrase (CA) catalyzes the first biochemical step of the carbon concentrating mechanism of C4 plants, and in C4 monocots, it has been suggested that CA activity is near limiting for photosynthesis. Here, we test this hypothesis through the characterization of transposon induced mutant alleles of Ca1 and Ca2 in Zea mays. In addition, these two isoforms account for more than 85% of the CA transcript pool. A significant change in isotopic discrimination is observed in mutant plants, which have as little as 3% of wild-type CA activity, but surprisingly, photosynthesis is not reduced under current or elevated pCO2. However,more » growth and rates of photosynthesis under sub-ambient pCO2 are significantly impaired in the mutants. These findings suggest, that while CA is not limiting for C4 photosynthesis in Z. mays at current pCO2, it likely maintains high rates of photosynthesis when CO2 availability is reduced. Current atmospheric CO2 levels now exceed 400 ppm (~40.53 Pa) and contrast the low CO2 partial pressure (pCO2) conditions under which C4 plants expanded their range ~10 million years ago when the global atmospheric CO2 was below 300 ppm (~30.40 Pa). Thus, as CO2 levels continue to rise, selective pressures for high levels of CA may be limited to arid climates where stomatal closure reduces CO2 availability to the leaf.« less

  11. Dark States in the Light-Harvesting complex 2 Revealed by Two-dimensional Electronic Spectroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ferretti, Marco; Hendrikx, Ruud; Romero, Elisabet; Southall, June; Cogdell, Richard J.; Novoderezhkin, Vladimir I.; Scholes, Gregory D.; van Grondelle, Rienk

    2016-02-09

    Energy transfer and trapping in the light harvesting antennae of purple photosynthetic bacteria is an ultrafast process, which occurs with a quantum efficiency close to unity. However the mechanisms behind this process have not yet been fully understood. Recently it was proposed that low-lying energy dark states, such as charge transfer states and polaron pairs, play an important role in the dynamics and directionality of energy transfer. However, it is difficult to directly detect those states because of their small transition dipole moment and overlap with the B850/B870 exciton bands. Here we present a new experimental approach, which combines themore » selectivity of two-dimensional electronic spectroscopy with the availability of genetically modified light harvesting complexes, to reveal the presence of those dark states in both the genetically modified and the wild-type light harvesting 2 complexes of Rhodopseudomonas palustris. In conclusion, we suggest that Nature has used the unavoidable charge transfer processes that occur when LH pigments are concentrated to enhance and direct the flow of energy.« less

  12. Increase in ethanol yield via elimination of lactate production in an ethanol-tolerant mutant of Clostridium thermocellum

    SciTech Connect (OSTI)

    Biswas, Ranjita; Prabhu, Sandeep; Lynd, Lee R; Guss, Adam M

    2014-01-01

    Large-scale production of lignocellulosic biofuel is a potential solution to sustainably meet global energy needs. One-step consolidated bioprocessing (CBP) is a potentially advantageous approach for the production of biofuels, but requires an organism capable of hydrolyzing biomass to sugars and fermenting the sugars to ethanol at commercially viable titers and yields. Clostridium thermocellum, a thermophilic anaerobe, can ferment cellulosic biomass to ethanol and organic acids, but low yield, low titer, and ethanol sensitivity remain barriers to industrial production. Here, we deleted the hypoxanthine phosphoribosyltransferase gene in ethanol tolerant strain of C. thermocellum adhE*(EA) in order to allow use of previously developed gene deletion tools, then deleted lactate dehydrogenase (ldh) to redirect carbon flux towards ethanol. Upon deletion of ldh, the adhE*(EA) ldh strain produced 30% more ethanol than wild type on minimal medium. The adhE*(EA) ldh strain retained tolerance to 5% v/v ethanol, resulting in an ethanol tolerant platform strain of C. thermocellum for future metabolic engineering efforts.

  13. A comprehensive collection of systems biology data characterizing the host response to viral infection

    SciTech Connect (OSTI)

    Aevermann, Brian D.; Pickett, Brett E.; Kumar, Sanjeev; Klem, Edward B.; Agnihothram, Sudhakar; Askovich, Peter S.; Bankhead, Armand; Bolles, Meagan; Carter, Victoria; Chang, Jean H.; Clauss, Therese R. W.; Dash, Pradyot; Diercks, Alan H.; Eisfeld, Amie J.; Ellis, Amy L.; Fan, Shufang; Ferris, Martin T.; Gralinski, Lisa; Green, Richard; Gritsenko, Marina A.; Hatta, Masato; Heegel, Robert A.; Jacobs, Jon M.; Jeng, Sophia; Josset, Laurence; Kaiser, Shari M.; Kelly, Sarah; Law, Gale Lynn; Li, Chengjun; Li, Jiangning; Long, Casey; Luna, Maria L.; Matzke, Melissa M.; McDermott, Jason E.; Menachery, Vineet; Metz, Thomas O.; Mitchell, Hugh D.; Monroe, Matthew E.; Navarro, Garnet; Neumann, Gabriele; Podyminogin, Rebecca L.; Purvine, Samuel O.; Rosenberger, Carrie; Sanders, Catherine J.; Schepmoes, Athena A.; Shukla, Anil K.; Sims, Amy; Sova, Pavel; Tam, Vincent C.; Tchitchek, Nicholas; Thomas, Paul G.; Tilton, Susan C.; Totura, Allison L.; Wang, Jing; Webb-Robertson, Bobbie-Jo M.; Wen, Ji; Weiss, Jeffrey M.; Yang, Feng; Yount, Boyd; Zhang, Qibin; Mcweeney, Shannon K.; Smith, Richard D.; Waters, Katrina M.; Kawaoka, Yoshihiro; Baric, Ralph; Aderem, Alan; Katze, Michael G.; Scheuermann, Richard H.

    2014-10-14

    The Systems Biology for Infectious Diseases Research program was established by the U.S. National Institute of Allergy and Infectious Diseases to investigate host-pathogen interactions at a systems level. This program generated 47 transcriptomic and proteomic datasets from 30 studies that investigate in vivo and in vitro host responses to viral infections. Human pathogens in the Orthomyxoviridae and Coronaviridae families, especially pandemic H1N1 and avian H5N1 influenza A viruses and severe acute respiratory syndrome coronavirus (SARS-CoV), were investigated. Study validation was demonstrated via experimental quality control measures and meta-analysis of independent experiments performed under similar conditions. Primary assay results are archived at the GEO and PeptideAtlas public repositories, while processed statistical results together with standardized metadata are publically available at the Influenza Research Database (www.fludb.org) and the Virus Pathogen Resource (www.viprbrc.org). As a result, by comparing data from mutant versus wild-type virus and host strains, RNA versus protein differential expression, and infection with genetically similar strains, these data can be used to further investigate genetic and physiological determinants of host responses to viral infection.

  14. Evolution of context dependent regulation by expansion of feast/famine regulatory proteins

    SciTech Connect (OSTI)

    Plaisier, Christopher L.; Lo, Fang -Yin; Ashworth, Justin; Brooks, Aaron N.; Beer, Karlyn D.; Kaur, Amardeep; Pan, Min; Reiss, David J.; Facciotti, Marc T.; Baliga, Nitin S.

    2014-11-14

    Expansion of transcription factors is believed to have played a crucial role in evolution of all organisms by enabling them to deal with dynamic environments and colonize new environments. We investigated how the expansion of the Feast/Famine Regulatory Protein (FFRP) or Lrp-like proteins into an eight-member family in Halobacterium salinarum NRC-1 has aided in niche-adaptation of this archaeon to a complex and dynamically changing hypersaline environment. We mapped genome-wide binding locations for all eight FFRPs, investigated their preference for binding different effector molecules, and identified the contexts in which they act by analyzing transcriptional responses across 35 growth conditions that mimic different environmental and nutritional conditions this organism is likely to encounter in the wild. Integrative analysis of these data constructed an FFRP regulatory network with conditionally active states that reveal how interrelated variations in DNA-binding domains, effector-molecule preferences, and binding sites in target gene promoters have tuned the functions of each FFRP to the environments in which they act. We demonstrate how conditional regulation of similar genes by two FFRPs, AsnC (an activator) and VNG1237C (a repressor), have striking environment-specific fitness consequences for oxidative stress management and growth, respectively. This study provides a systems perspective into the evolutionary process by which gene duplication within a transcription factor family contributes to environment-specific adaptation of an organism.

  15. Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dao, E. Han; Sierra, Raymond G.; Laksmono, Hartawan; Lemke, Henrik T.; Alonso-Mori, Roberto; Coey, Aaron; Larsen, Kevin; Baxter, Elizabeth L.; Cohen, Aina E.; Soltis, S. Michael; et al

    2015-04-30

    In this work, we collected radiation-damage-free data from a set of cryo-cooled crystals for a novel 30S ribosomal subunit mutant using goniometer-based femtosecond crystallography. Crystal quality assessment for these samples was conducted at the X-ray Pump Probe end-station of the Linac Coherent Light Source (LCLS) using recently introduced goniometer-based instrumentation. These 30S subunit crystals were genetically engineered to omit a 26-residue protein, Thx, which is present in the wild-type Thermus thermophilus 30S ribosomal subunit. We are primarily interested in elucidating the contribution of this ribosomal protein to the overall 30S subunit structure. To assess the viability of this study, femtosecondmore » X-ray diffraction patterns from these crystals were recorded at the LCLS during a protein crystal screening beam time. During our data collection, we successfully observed diffraction from these difficult-to-grow 30S ribosomal subunit crystals. Most of our crystals were found to diffract to low resolution, while one crystal diffracted to 3.2 Å resolution. These data suggest the feasibility of pursuing high-resolution data collection as well as the need to improve sample preparation and handling in order to collect a complete radiation-damage-free data set using an X-ray Free Electron Laser.« less

  16. Small acid soluble proteins for rapid spore identification.

    SciTech Connect (OSTI)

    Branda, Steven S.; Lane, Todd W.; VanderNoot, Victoria A.; Jokerst, Amanda S.

    2006-12-01

    This one year LDRD addressed the problem of rapid characterization of bacterial spores such as those from the genus Bacillus, the group that contains pathogenic spores such as B. anthracis. In this effort we addressed the feasibility of using a proteomics based approach to spore characterization using a subset of conserved spore proteins known as the small acid soluble proteins or SASPs. We proposed developing techniques that built on our previous expertise in microseparations to rapidly characterize or identify spores. An alternative SASP extraction method was developed that was amenable to both the subsequent fluorescent labeling required for laser-induced fluorescence detection and the low ionic strength requirements for isoelectric focusing. For the microseparations, both capillary isoelectric focusing and chip gel electrophoresis were employed. A variety of methods were evaluated to improve the molecular weight resolution for the SASPs, which are in a molecular weight range that is not well resolved by the current methods. Isoelectric focusing was optimized and employed to resolve the SASPs using UV absorbance detection. Proteomic signatures of native wild type Bacillus spores and clones genetically engineered to produce altered SASP patterns were assessed by slab gel electrophoresis, capillary isoelectric focusing with absorbance detection as well as microchip based gel electrophoresis employing sensitive laser-induced fluorescence detection.

  17. Methylotroph cloning vehicle

    DOE Patents [OSTI]

    Hanson, Richard S.; Allen, Larry N.

    1989-04-25

    A cloning vehicle comprising: a replication determinant effective for replicating the vehicle in a non-C.sub.1 -utilizing host and in a C.sub.1 -utilizing host; DNA effective to allow the vehicle to be mobilized from the non-C.sub.1 -utilizing host to the C.sub.1 -utilizing host; DNA providing resistance to two antibiotics to which the wild-type C.sub.1 -utilizing host is susceptible, each of the antibiotic resistance markers having a recognition site for a restriction endonuclease; a cos site; and a means for preventing replication in the C.sub.1 -utilizing host. The vehicle is used for complementation mapping as follows. DNA comprising a gene from the C.sub.1 -utilizing organism is inserted at the restriction nuclease recognition site, inactivating the antibiotic resistance marker at that site. The vehicle can then be used to form a cosmid structure to infect the non-C.sub.1 -utilizing (e.g., E. coli) host, and then conjugated with a selected C.sub.1 -utilizing mutant. Resistance to the other antibiotic by the mutant is a marker of the conjugation. Other phenotypical changes in the mutant, e.g., loss of an auxotrophic trait, is attributed to the C.sub.1 gene. The vector is also used to inactivate genes whose protein products catalyze side reactions that divert compounds from a biosynthetic pathway to a desired product, thereby producing an organism that makes the desired product in higher yields.

  18. Decreased cell adhesion promotes angiogenesis in a Pyk2-dependent manner

    SciTech Connect (OSTI)

    Shen, Colette J.; Raghavan, Srivatsan; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205 ; Xu, Zhe; Baranski, Jan D.; Yu, Xiang; Wozniak, Michele A.; Miller, Jordan S.; Gupta, Mudit; Buckbinder, Leonard; Chen, Christopher S.

    2011-08-01

    Angiogenesis is regulated by both soluble growth factors and cellular interactions with the extracellular matrix (ECM). While cell adhesion via integrins has been shown to be required for angiogenesis, the effects of quantitative changes in cell adhesion and spreading against the ECM remain less clear. Here, we show that angiogenic sprouting in natural and engineered three-dimensional matrices exhibited a biphasic response, with peak sprouting when adhesion to the matrix was limited to intermediate levels. Examining changes in global gene expression to determine a genetic basis for this response, we demonstrate a vascular endothelial growth factor (VEGF)-induced upregulation of genes associated with vascular invasion and remodeling when cell adhesion was limited, whereas cells on highly adhesive surfaces upregulated genes associated with proliferation. To explore a mechanistic basis for this effect, we turned to focal adhesion kinase (FAK), a central player in adhesion signaling previously implicated in angiogenesis, and its homologue, proline-rich tyrosine kinase 2 (Pyk2). While FAK signaling had some impact, our results suggested that Pyk2 can regulate both gene expression and endothelial sprouting through its enhanced activation by VEGF in limited adhesion contexts. We also demonstrate decreased sprouting of tissue explants from Pyk2-null mice as compared to wild type mice as further confirmation of the role of Pyk2 in angiogenic sprouting. These results suggest a surprising finding that limited cell adhesion can enhance endothelial responsiveness to VEGF and demonstrate a novel role for Pyk2 in the adhesive regulation of angiogenesis.

  19. Tailoring a low-molecular weight protein tyrosine phosphatase into an efficient reporting protein

    SciTech Connect (OSTI)

    Liu, Xiao-Yan; Li, Lan-Fen [The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871 (China)] [The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871 (China); Su, Xiao-Dong, E-mail: xdsu@pku.edu.cn [The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871 (China) [The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871 (China); Shenzhen Graduate School of Peking University, Shenzhen 518055 (China)

    2009-05-15

    Fusion reporter methods are important tools for biology and biotechnology. An ideal reporter protein in a fusion system should have little effects on its fusion partner and provide an easy and accurate readout. Therefore, a small monomeric protein with high activity for detection assays often has advantages as a reporter protein. For this purpose, we have tailored the human B-form low-molecular-weight phosphotyrosyl phosphatase (HPTP-B) to increase its general applicability as a potent reporter protein. With the aim to eliminate interference from cysteine residues in the native HPTP-B, combined with a systematic survey of N- and C-terminal truncated variants, a series of cysteine to serine mutations were introduced, which allowed isolation of an engineered soluble protein with suitable biophysical properties. When we deleted both the first six residues and the last two residues, we still obtained a soluble mutant protein with correct folding and similar activity with wild-type protein. This mutant with two cysteine to serine mutations, HPTP-B{sup N{sub {Delta}}6-C{sub {Delta}}2-C90S-C109S}, has good potential as an optimal reporter.

  20. A Survey of lamba Repressor Fragments from Two-State to to Downhill Folding

    SciTech Connect (OSTI)

    Liu, F.; Gao, Y; Gruebele, M

    2010-01-01

    We survey the two-state to downhill folding transition by examining 20 {lambda}{sub 6-85}* mutants that cover a wide range of stabilities and folding rates. We investigated four new {lambda}{sub 6-85}* mutants designed to fold especially rapidly. Two were engineered using the core remodeling of Lim and Sauer, and two were engineered using Ferreiro et al.'s frustratometer. These proteins have probe-dependent melting temperatures as high as 80 C and exhibit a fast molecular phase with the characteristic temperature dependence of the amplitude expected for downhill folding. The survey reveals a correlation between melting temperature and downhill folding previously observed for the {beta}-sheet protein WW domain. A simple model explains this correlation and predicts the melting temperature at which downhill folding becomes possible. An X-ray crystal structure with a 1.64-{angstrom} resolution of a fast-folding mutant fragment shows regions of enhanced rigidity compared to the full wild-type protein.