National Library of Energy BETA

Sample records for hakes 1993-2000 calvin

  1. Melvin Calvin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Melvin Calvin About the Lab Our Vision Lab Leadership History Nobelists Visit ⇒ Navigate Section About the Lab Our Vision Lab Leadership History Nobelists Visit calvin 1961 Nobel Prize for Chemistry * Presentation of Award * Acceptance Speech * Biography Submitted by Dr. Calvin to the Nobel Committee * Presentation of Award: 1961 Nobel Prize for Chemistry Professor K. Myrbaumlck, member of the Swedish Academy of Sciences: Your Majesties, Your Royal Highnesses, Ladies and Gentlemen. In order to

  2. About EIA - History - U.S. Energy Information Administration (EIA)

    Gasoline and Diesel Fuel Update (EIA)

    Previous Administrators Richard Newell Guy Caruso Jay Hakes Calvin Kent Richard Newell 2009-2011 Guy Caruso 2002-2008 Jay Hakes 1993-2000 Calvin Kent 1990-1993 Helmut Merklein Erich Evered Lincoln Moses Helmut Merklein 1985-1990 Erich Evered 1981-1984 Lincoln Moses 1978-1980

  3. Slide 1

    Gasoline and Diesel Fuel Update (EIA)

    Guy Caruso 2002- Present Helmut Merklein 1985-1990 EIA Administrators 1978- Present Erich Evered 1981-1984 Lincoln Moses 1978-1980 Jay Hakes 1993-2000 Calvin Kent 1990-1993

  4. Melvin Calvin and Carbon in Photosynthesis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Melvin Calvin and Carbon in Photosynthesis Resources with Additional Information Melvin Calvin Courtesy Lawrence Berkeley National Laboratory 'On September 2, 1945, ... Ernest Lawrence, Director of UC Berkeley's Radiation Laboratory, suggested to chemistry professor and "Rad Lab" researcher Melvin Calvin that "now is the time to do something useful with radioactive carbon." That nudge eventually led Calvin to uncover the secrets of how plants capture energy from the sun. The

  5. Dr. Calvin O. Butts, III | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Calvin O. Butts, III About Us Dr. Calvin O. Butts, III - President, State University of New York (SUNY) College at Old Westbury Dr. Calvin O. Butts, III Dr. Calvin O. Butts, III, is President of State University of New York, College at Old Westbury and Pastor of the renowned Abyssinian Baptist Church in New York City. Regularly sought by leaders in politics, business, and the media for his insight and opinions, he has had a pervasive impact across his career on such wide-ranging issues as

  6. Pantex Plant's Calvin Nelson honored as Analyst of the Year for

    National Nuclear Security Administration (NNSA)

    Transportation Security | National Nuclear Security Administration Plant's Calvin Nelson honored as Analyst of the Year for Transportation Security | National Nuclear Security Administration Facebook Twitter Youtube Flickr RSS People Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Countering Nuclear Terrorism About Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations

  7. Calvin 2015 Peer Review Poster Reduced

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    gic-mechanical-chemical coupled modeling to investigate fracture network permeability evolution during enhanced geothermal system stimulation at Desert Peak, Nevada PI Matt Reeves,...

  8. Hyperspectral Imaging At Blue Mountain Geothermal Area (Calvin...

    Open Energy Info (EERE)

    layered silicates, opal, calcite, zeolites, and iron oxides and hydroxides. The experimental technique may be able to sample drill cuttings and core more efficiently than...

  9. The Fall Meeting of the Committee on Energy Statistics

    U.S. Energy Information Administration (EIA) Indexed Site

    * * * * * FRIDAY NOVEMBER 5, 1999 The Fall Meeting of the Committee on Energy Statistics commenced at 8:30 a.m. at the Department of Energy, 1000 Independence Avenue, S.W., Room 8E089, Washington, D.C., Daniel Relles, presiding. PRESENT: DANIEL RELLES, Chairman JAY BREIDT LYNDA CARLSON THOMAS COWING CAROL GOTWAY CRAWFORD JAY HAKES JAMES HAMMITT PHILIP HANSER CALVIN KENT W. DAVID MONTGOMERY LARRY PETTIS SEYMOUR SUDMAN BILL WEINIG ROY WHITMORE C O N T E N T S PAGE Opening 5 Addressing Accuracy in

  10. C:\\ANNUAL\\Vol2chps.v8\\ANNUAL2.VP

    U.S. Energy Information Administration (EIA) Indexed Site

    42 32. Average Price of Natural Gas Delivered to U.S. Industrial Consumers by State, 1993-2000 (Dollars per Thousand Cubic Feet) Table State 1993 1994 1995 1996 Average Price % of...

  11. Rapid characterization of drill core and cutting mineralogy using...

    Open Energy Info (EERE)

    Authors W. Calvin, A. Lamb and C. Kratt Published Journal Geothermal Resources Council Transactions, 2010 DOI Not Provided Check for DOI availability: http:crossref.org...

  12. Hyperspectral Imaging At Fish Lake Valley Area (Littlefield ...

    Open Energy Info (EERE)

    Fish Lake Valley Area (Littlefield & Calvin, 2010) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Hyperspectral Imaging At Fish Lake Valley Area...

  13. The Silicon Solar Cell Turns 50

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Daryl Chapin, Calvin Fuller, and Gerald Pearson likely never imagined inventing a solar cell that would revolutionize the photovoltaics industry. There wasn't even a photovoltaics...

  14. TUNL Master Theses

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    a High Precision 19Ne Lifetime Measurement. Duke University, April 2007, Supervisors: Albert Young (NC State Univ.) and Calvin R. Howell. (Duke Univ.) Download PDF Cynthia L....

  15. Controlled Covalent Modification of Epitaxial Single Layer Graphene...

    Office of Scientific and Technical Information (OSTI)

    Abstract not provided. Authors: Chan, Calvin ; Ohta, Taisuke ; Stevenson, Keith ; Veneman, Peter ; Gearba, Raluca ; Mueller, Kory ; Holliday, Bradley Publication Date: 2014-03-01 ...

  16. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Chan, Calvin ; Beechem Iii, Thomas Edwin ; Ohta, Taisuke ; Brumbach, Michael T. ; Wheeler, David Roger ; Veneman, Alexander ; Gearba, I. Raluca ; Stevenson, Keith J. Chemical ...

  17. Polycrystalline TiO2(B) Nanosheet Films Deposited via Langmuir...

    Office of Scientific and Technical Information (OSTI)

    Authors: Biedermann, Laura ; Kotula, Paul Gabriel ; Beechem Iii, Thomas Edwin ; Chan, Calvin ; Dylla, Anthony ; Stevenson, Keith Publication Date: 2014-03-01 OSTI Identifier: ...

  18. University of california | Open Energy Information

    Open Energy Info (EERE)

    search Name: Energy Biosciences Institute Address: 146 Calvin Laboratory Place: Berkeley, California Zip: 94720 Region: Bay Area Number of Employees: 501-1000 Year Founded:...

  19. NREL: Energy Sciences Home Page

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    define the strategic direction of our research. Fellows Angelo Mascarenhas Garry Rumbles John Turner Emeriti Calvin (Buzz) Curtis Satyen Deb Arthur Frank Larry Kazmerski Arthur...

  20. NREL: Energy Sciences - Chemical and Materials Science Staff

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the strategic direction of our research. Fellows Angelo Mascarenhas Garry Rumbles John Turner Emeriti Calvin (Buzz) Curtis Satyen Deb Arthur Frank Larry Kazmerski Arthur...

  1. Triangle Universities Nuclear Laboratory : 2011

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Astrophysics Art Champagne Calvin Howell Hadronic Parity Violation (PV) Mike Snow Roxanne Springer PV Working Group Beyond the Standard Model Reyco Henning Jesse Thaler HIGS2...

  2. NREL Demonstrates Photocatalytic Conversion With Mutant Microbe...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cells (red arrows) under nitrogen-deprived conditions. Carbon (as CO 2 ) enters the Calvin-Benson- Bassham (CBB) cycle to produce metabolic intermediate carbon compounds...

  3. Cavalier County, North Dakota: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Calvin, North Dakota Hannah, North Dakota Langdon, North Dakota Loma, North Dakota Milton, North Dakota Munich, North Dakota Nekoma, North Dakota Osnabrock, North Dakota...

  4. OSTI, US Dept of Energy, Office of Scientific and Technical Informatio...

    Office of Scientific and Technical Information (OSTI)

    David Gross, DOE Research & Development (R&D) Accomplishments, E.O. Lawrence, Glenn Seaborg, human genome, Manhattan Project, Melvin Calvin, nobel laureates, Saul Perlmutter, space...

  5. Office of Secure Transportation Celebrates 40th Anniversary ...

    National Nuclear Security Administration (NNSA)

    of 2 Related Topics ost NNSA transportation Related News Pantex Plant's Calvin Nelson honored as Analyst of the Year for Transportation Security Acquisition and Project...

  6. Biomass Deconstruction: Catalyst Development and Testing Presentation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Gordon Weatherbee (WR Grace) Mike Watson, Andrew Heavers (Johnson Matthey) Larry ... 4. Calvin Mukarakate, Michael Watson, Jeroen ten Dam, Xavier Baucherel, ...

  7. Search for: All records | DOE Patents

    Office of Scientific and Technical Information (OSTI)

    SapuriButti, Annapoorna R. (1) Swanson, Basil I. (1) Yang, Calvin (1) Yee, Chanel Kitmon (1) Save Results Excel (limit 2000) CSV (limit 5000) XML (limit 5000) Have feedback or ...

  8. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Kyle, G. Page (10) Kim, Son H. (8) Eom, Jiyong (7) Smith, Steven J. (7) Calvin, Katherine ... Costs Zhou, Yuyu ; Luckow, Patrick ; Smith, Steven J. ; Clarke, Leon E. In this ...

  9. award

    National Nuclear Security Administration (NNSA)

    about these awards on the Y-12 website.

    Pantex Plant's Calvin Nelson honored as Analyst of the Year for...

  10. security

    National Nuclear Security Administration (NNSA)

    exan-Calvin-Nelson-secures-recognition-for-expertise.aspx">Pantex website.

    Apex Gold discussion fosters international cooperation in run-up to 2016 Nuclear Security Summit...

  11. transportation

    National Nuclear Security Administration (NNSA)

    security missions undertaken by the U.S. government.

    Pantex Plant's Calvin Nelson honored as Analyst of the Year for Transportation Security http:nnsa.energy.gov...

  12. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UNIVERSIT Y OF CALIFORNIA Lawrence Radiation Laboratory Berkeley, California Contract No. W -740S-eng -48 UCRL-9966 I THE PATH OF CARBON IN PHOTOSYNTHESIS Melvin Calvin Nobel Prize Lecture December 11, 1961 ) Nobel Prize Lecture December 11, 1961 UCRL-9966 THE PATH OF Ck'1BON IN PHOI'CBYHTHESIS Melvin Calvin Department of Chemistry and Lawrence Radiation Laboratory University of California, Berkeley 4, California ll'JTRODUCTION It is almost sixty years since Emil Fischer was describing on 8

  13. Triangle Universities Nuclear Laboratory : 2011

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TUNL Management and Advisory Committees Calvin Howell, Director Paul Huffman, Associate Director John Wilkerson, Associate Director Henry Weller, Associate Director for Nuclear Physics at HIγS Ying Wu, Associate Director for Light Sources TUNL Scientific Steering Committee (TSSC) Calvin Howell Werner Tornow Paul Hufman John Wilkerson TUNL Advisory Committee (TAC) - as of January 2014 Steven Vigdor (Chair), Indiana University Bloomington Ani Aprahamian, University of Notre Dame Judith McGovern,

  14. Boise State University | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Boise State University Boise State University Top left: Mike Sansorm, Calvin Brown, Cody McConkey, Luke Weaver. Top right: Cameron Allen, Scott Roskens, Mitchell Petronek, Davis Gumbo. Bottom Left: Jerad Deitrick, Brandon Lee, Nael Naser, Luke Ganschow. Bottom middle: Grant Stephens, Michael Shoaee, Brian Cardwell, Rory O'Leary. Bottom right: Brian Dambi, Stephan Stuats, Adrian Reyes, Haitian Xu, Firaj Almasyabi. Photo from Boise State University. Top left: Mike Sansorm, Calvin Brown, Cody

  15. Worldwide Natural Gas Supply and Demand and the Outlook for Global LNG Trade

    Reports and Publications (EIA)

    1997-01-01

    This article is adapted from testimony by Jay Hakes, Administrator of the Energy Information Administration, before the Senate Energy and Natural Resources Committee on July 23, 1997. The hearing focused on the examination of certain aspects of natural gas into the next century with special emphasis on world natural gas supply and demand to 2015.

  16. C:\ANNUAL\Vol2chps.v8\ANNUAL2.VP

    Gasoline and Diesel Fuel Update (EIA)

    4 Energy Information Administration / Historical Natural Gas Annual 1930 Through 2000 15. Consumption of Natural Gas by State, 1993-2000 (Million Cubic Feet) Table State Delivered to Consumers Lease Fuel Plant Fuel Pipeline Fuel Total Consumption 1993 Alabama .................................... 263,421 8,912 4,453 15,559 292,346 Alaska ....................................... 137,680 202,199 35,503 2,859 378,241 Arizona ...................................... 97,417 49 0 17,339 114,806 Arkansas

  17. Improving carbon fixation pathways

    SciTech Connect (OSTI)

    Ducat, DC; Silver, PA

    2012-08-01

    A recent resurgence in basic and applied research on photosynthesis has been driven in part by recognition that fulfilling future food and energy requirements will necessitate improvements in crop carbon-fixation efficiencies. Photosynthesis in traditional terrestrial crops is being reexamined in light of molecular strategies employed by photosynthetic microbes to enhance the activity of the Calvin cycle. Synthetic biology is well-situated to provide original approaches for compartmentalizing and enhancing photosynthetic reactions in a species independent manner. Furthermore, the elucidation of alternative carbon-fixation routes distinct from the Calvin cycle raises possibilities that novel pathways and organisms can be utilized to fix atmospheric carbon dioxide into useful materials.

  18. 1000 Independence Avenue, SW, Washington, D.C., Carol G. Crawford, Chair,

    U.S. Energy Information Administration (EIA) Indexed Site

    FRIDAY, OCTOBER 25, 2002 The meeting was held at 8:30 in Room 8E-089 of the Department of Energy, 1000 Independence Avenue, SW, Washington, D.C., Carol G. Crawford, Chair, presiding. PRESENT: CAROL G. CRAWFORD, Ph.D. Chair F. JAY BREIDT, Ph.D. Vice Chair MARK BERNSTEIN, Ph.D. JOHNNY BLAIR JAE EDMONDS, Ph.D. JAMES K. HAMMITT, Ph.D. NICHOLAS W. HENGARTNER CALVIN A. KENT, Ph.D. WILLIAM G. MOSS, Ph.D. POLLY A. PHIPPS, Ph.D. RANDY R. SITTER, Ph.D. ROY WHITMORE, Ph.D. ALSO PRESENT: CALVIN A. KENT,

  19. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UCRd1950 Unclassified-Chemistry Distribution U P I r n I T Y OF CALIFORNIA Radiation Laboratory Contract No. V-7405-eng-48 THE PATH OF CllRBOM I N PHOTOSYNTHFSIS. XX. THE STEADY STATE M. Calvin and Peter Massini September, 1952 Berkeley, California THE PATH O F C A R B O N IA? PHOTOSYfJTHESIS, no TKE STEWY S T A T E E 35 M a Calvin and Peter h s s f n i Radiation Labmatmy and Deparktienfi of Chemistry7 University of D a l i f ornia, ~crkeley** (*) Fellow of the Swias Foundation, mStStiftunk f k

  20. Slide 1

    Gasoline and Diesel Fuel Update (EIA)

    External Study Team: Introduction and Evaluation A. Denny Ellerman Center for Energy and Environmental Policy Analysis Massachusetts Institute of Technology 2008 EIA Energy Conference April 7, 2008 External Study Team Introduction * Who? * Leader: A. Denny Ellerman * Members: Kathleen B. Cooper Jay Hakes Paul L. Joskow Philip Sharp * What? * Evaluate EIA's activities & performance * Consider is EIA doing the "right things" * Identify challenges for next five years * When? * June

  1. C:\ANNUAL\Vol2chps.v8\ANNUAL2.VP

    Gasoline and Diesel Fuel Update (EIA)

    0 29. Average Price of Natural Gas Delivered to U.S. Commercial Consumers by State, 1993-2000 (Dollars per Thousand Cubic Feet) Table State 1993 1994 1995 1996 Average Price % of Total Volume Delivered Average Price % of Total Volume Delivered Average Price % of Total Volume Delivered Average Price % of Total Volume Delivered Alabama ................ 6.19 80.8 6.38 80.2 5.80 80.1 6.19 81.1 Alaska.................... 2.78 100.0 2.48 100.0 2.27 79.9 2.32 63.4 Arizona .................. 5.06 91.5

  2. C:\ANNUAL\Vol2chps.v8\ANNUAL2.VP

    Gasoline and Diesel Fuel Update (EIA)

    42 32. Average Price of Natural Gas Delivered to U.S. Industrial Consumers by State, 1993-2000 (Dollars per Thousand Cubic Feet) Table State 1993 1994 1995 1996 Average Price % of Total Volume Delivered Average Price % of Total Volume Delivered Average Price % of Total Volume Delivered Average Price % of Total Volume Delivered Alabama ................ 3.28 27.5 3.26 27.3 2.96 23.4 3.64 22.6 Alaska.................... 1.29 70.6 1.42 58.4 1.45 52.1 1.41 64.3 Arizona .................. 4.02 25.0

  3. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For a Tech. This is a Library Circulating Copy which may be borrowed for t w o weeks. personal retention copy, call Info. Division, Ext. 5545 BERKELEY, CALIFORNIA U-i~IvERSITY 03' C k L I F O r n l k R a d i a t i o n L a b o r a t o r y Contract flo. '?1'-7405-t?ng-48 m PATH OF C r n O r J ITJ F'HOTOS'rnESIS. VI . ?ii. Calvin June 30, 1949 Berkeley , California THE PATH OF CkRBON IN PHOTOSYNTHESIS. VI.* Ni. Calvin Radiation Laboratory and Department o f Chemistry, University of California,

  4. Boise State University: Technical Design Report

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    VENTO: Wind Power Generator Design Portfolio Boise State University Spring 2014 Michael Sansom 1 , Luke Weaver 1 , Cody McConkey 1 , Calvin Brown 1 , Stephen Stauts 2 , Brian Dambi 2 , Adrian Reyes 2 , Haitian Xu 2 , Firas Almasyabi 2 , John Gardner, Ph.D. 1 , Sarah Haight 1 , Eric Booth 2 , Matthew Dolan 2 1 Department of Mechanical and Biomedical Engineering 2 Department of Electrical and Computer Engineering Vento: Wind Turbine Education Kits Business Plan Portfolio Team Members and Contacts

  5. Sabourov_2006 (much larger file).pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Amanda Sabourov 2006 Understanding the 7 Li(d, n) 8 Be Reaction at Very Low Energies by Amanda Sabourov Department of Physics Duke University Date Approved: Henry R. Weller, Supervisor Shailesh Chandrasekharan Haiyan Gao Calvin Howell Ashutosh Kotwal Dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 2006 Abstract (Physics - TUNL) Understanding the 7 Li(d, n) 8 Be Reaction

  6. Abstracts for EIA's Spring 2008 Meeting with the

    Gasoline and Diesel Fuel Update (EIA)

    Spring 2008 Meeting with the ASA Committee on Energy Statistics 1. The Role of A Federal Advisory Committee: A Case Study of the American Statistical Association (ASA) Committee on Energy Statistics, Calvin A. Kent, Former EIA Administrator and Dean of Lewis College of Business, Marshall University The energy crisis of the 1970's brought about the creation of the U. S. Department of Energy, which was created by congress in 1977. As a statistical organization within the Department of Energy, the

  7. High Precision Measurement of the 19Ne Lifetime

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Precision Measurement of the 19 Ne Lifetime by Leah Jacklyn Broussard Department of Physics Duke University Date: Approved: Albert Young Calvin Howell Kate Scholberg Berndt Mueller John Thomas Dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 2012 Abstract (Nuclear physics) High Precision Measurement of the 19 Ne Lifetime by Leah Jacklyn Broussard Department of Physics

  8. Polycrystalline TiO2(B) Nanosheet Films Deposited via Langmuir-Blodgett

    Office of Scientific and Technical Information (OSTI)

    Method. (Conference) | SciTech Connect Polycrystalline TiO2(B) Nanosheet Films Deposited via Langmuir-Blodgett Method. Citation Details In-Document Search Title: Polycrystalline TiO2(B) Nanosheet Films Deposited via Langmuir-Blodgett Method. Abstract not provided. Authors: Biedermann, Laura ; Kotula, Paul Gabriel ; Beechem Iii, Thomas Edwin ; Chan, Calvin ; Dylla, Anthony ; Stevenson, Keith Publication Date: 2014-03-01 OSTI Identifier: 1140906 Report Number(s): SAND2014-1720C 505110 DOE

  9. Controlled Covalent Modification of Epitaxial Single Layer Graphene on

    Office of Scientific and Technical Information (OSTI)

    6H-SiC (0001) with Aryliodonium Salts using Electrochemical Methods. (Journal Article) | SciTech Connect Journal Article: Controlled Covalent Modification of Epitaxial Single Layer Graphene on 6H-SiC (0001) with Aryliodonium Salts using Electrochemical Methods. Citation Details In-Document Search Title: Controlled Covalent Modification of Epitaxial Single Layer Graphene on 6H-SiC (0001) with Aryliodonium Salts using Electrochemical Methods. Abstract not provided. Authors: Chan, Calvin ;

  10. Controlled Covalent Modification of Epitaxial Single Layer Graphene on

    Office of Scientific and Technical Information (OSTI)

    6H-SiC (0001) with Aryliodonium Salts using Electrochemical Methods. (Journal Article) | SciTech Connect Journal Article: Controlled Covalent Modification of Epitaxial Single Layer Graphene on 6H-SiC (0001) with Aryliodonium Salts using Electrochemical Methods. Citation Details In-Document Search Title: Controlled Covalent Modification of Epitaxial Single Layer Graphene on 6H-SiC (0001) with Aryliodonium Salts using Electrochemical Methods. Abstract not provided. Authors: Chan, Calvin ;

  11. phd.dvi

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    EXTRACTION OF THE 1 S 0 NEUTRON-NEUTRON SCATTERING LENGTH FROM THE REACTION d ! nn USING MONTE-CARLO SIMULATION by Qiankun Chen Department of Physics Duke University Date: Approved: Richard L. Walter, Co-Supervisor Calvin R. Howell, Co-Supervisor Seog Oh Richard G. Palmer Werner Tornow Dissertation submitted in partial ful llment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 1998 ABSTRACT (Physics | Nuclear)

  12. thesis.dvi

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Proton Capture on Tritium at Proton Energies below 80 KeV by Richard Shane Canon Department of Physics Duke University Date Approved: Henry R. Weller, Supervisor Berndt Mueller Calvin Howell Vladimir Litvinenko Stephen Teitsworth Dissertation submitted in partial ful llment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 2001 Abstract (Physics { TUNL) Polarized Proton Capture on Tritium at Proton Energies below 80

  13. Cross-Section Measurements of Star Configurations in Neutron-Deuteron Breakup at 16.0 MeV

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Alexander S. Crowell 2001 Cross-Section Measurements of Star Configurations in Neutron-Deuteron Breakup at 16.0 MeV by Alexander S. Crowell Department of Physics Duke University Date Approved: Calvin R. Howell, Supervisor Robert P. Behringer Mark C. Kruse Roxanne P. Springer Werner Tornow Dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 2001 Abstract (Physics - TUNL)

  14. NREL: Energy Analysis - Andrew Weekley

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Andrew Weekley Photo of Andrew Weekley (UCAR, photo by Carlye Calvin) Andrew Weekley is a member of the Energy Forecasting and Modeling Group in the Strategic Energy Analysis Center. Data Scientist On staff since January 2013 Phone number: 303-275-3680 E-mail: andrew.weekley@nrel.gov Areas of expertise MATLAB Expert systems Scientific data analysis Primary research interests Time series segmentation and classification Image segmentation and classification Machine intelligent algorithms Education

  15. Triangle Universities Nuclear Laboratory : 2011

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Calvin Howell Alex Crowell Publications INSPIRES List Useful Links Duke Phytotron TUNL Studies in Plant Physiology Primary plant productivity sustains life on Earth and is a natural process for regulating atmospheric carbon dioxide (CO2) concentration. Many of the main features of plant growth and development can be modeled by treating plants as organisms composed of modular morphological subunits with physiological functionality, e.g., leaf, shoot and root. Each subunit performs a specialized

  16. Thermochemical Conversion: Using Heat and Catalysis to Make Biofuels and Bioproducts

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A wide variety of non-food biomass grown across the country can be converted into advanced hydrocarbon fuels using thermochemical processes. Photos (clockwise from upper left): iStock/3786400, Scott Butner/PNNL, iStock/6090867, Calvin Feik/NREL/16029 Thermochemical Conversion: Using Heat and Catalysis to Make Biofuels and Bioproducts The Bioenergy Technologies Offi ce works with industry and other partners to develop economical pathways that use heat, pressure, and catalysis to convert domestic,

  17. .*' UNIVJ3RSIl-Y OF CALIFORNIA, BERKELEY

    Office of Legacy Management (LM)

    ' .*' UNIVJ3RSIl-Y OF CALIFORNIA, BERKELEY March 4, 1981 Mr. Calvin A. Jackson, Director Environment, Safety and Program Support Division Department of Energy 1333 Broadway Oakland, California 94b12 Dear'Cal: Enclosed is my report.to our Radiation Safety Committee on the Burris Park situation. Please call if you have any questions. Sincerely, Andris Peterson Radiation Safety Officer AP:fo enc. vr- ' in ' ." ,' -, .,.-,.~"., ..,_ .."l,.l: .,....., _.,.-._._..,. .__.~..~,..~..

  18. High School Students Gear Up for Battle of the Brains - News Releases |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL High School Students Gear Up for Battle of the Brains February 3, 2003 Golden, CO. - In what part of the chloroplast is carbon fixed during the Calvin cycle? Yellow fever is transmitted by what genus of mosquito? High school students from across Colorado will face such questions as they test their mental agility in the U.S. Department of Energy's (DOE) Colorado Science Bowl on Feb. 8 at the Colorado School of Mines in Golden. Thirty-nine teams from across the state will compete in this

  19. The Committee met in the Columbia Room at the Holiday Inn

    U.S. Energy Information Administration (EIA) Indexed Site

    Friday, April 21, 1995 - - - The Committee met in the Columbia Room at the Holiday Inn Capitol, 550 C Street S.W., Washington, D.C., at 9:00 a.m., Timothy D. Mount, Chairman, presiding. PRESENT: TIMOTHY D. MOUNT, Chair DAVID R. BELLHOUSE CHARLES W. BISCHOFF BRENDA G. COX FAYE DUCHIN JOHN D. GRACE PHILIP HANSWER CALVIN KENT GRETA M. LJUNG JAMES L. O'BRIEN DANIEL A. RELLES BRADLEY O. SKARPNESS G. CAMPBELL WATKINS A-G-E-N-D-A Page No. Introductory Remarks, TIMOTHY MOUNT, Chairman 3 Announcement of

  20. OSTI, US Dept of Energy, Office of Scientific and Technical Information |

    Office of Scientific and Technical Information (OSTI)

    Speeding access to science information from DOE and Beyond Melvin Calvin Topic 100th DOE R&D Accomplishments Feature Page Celebration by Linda McBrearty 08 Jul, 2013 in Products and Content DOE R&D Accomplishments 100th Feature Page DOE R&D Accomplishments is a unique website and database in the OSTI collection. For over 14 years, special Feature pages have been methodically researched and useful information collected on scientists, discoveries, and historical events to include

  1. thesis.dvi

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Evidence for a Resonance at E p = 127 keV in the 14 N(p ) 15 O Reaction by Stephen O. Nelson Department of Physics Duke University Date Approved: Henry R. Weller, Supervisor Thomas Phillips Calvin Howell Henry Greenside Stephen Teitsworth Dissertation submitted in partial ful llment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 2002 Abstract (Physics { TUNL) Evidence for a Resonance at E p = 127 keV in the 14 N(p

  2. thesis.dvi

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Shigeyuki Tajima 2003 Measurements of the Electric Form Factor of the Neutron at Q 2 =0.45 and 1.13 (GeV/c) 2 by Shigeyuki Tajima Department of Physics Duke University Date Approved: Calvin R. Howell, Supervisor Richard Madey, Co-Supervisor Ashutosh V. Kotwal Roxanne P. Springer Stephen W. Teitsworth Richard L. Walter Dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Physics in the Graduate School of Duke University 2003

  3. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    I For a personal retention copy, call Tech. Info. Diuision. Ext. 5545 - UNIVEKSITY O F CALIFOKNIA Radiation Laboratory Contract No. W-7405-eng-48 UCRL-658 Unclassified D i s t r i b u t i o n The Fath of Carbon i n Photosynthesis X. Carbon Dioxide Assimilation i n F l a n t s U. Calvin, 3 , A . Bassham, A. A . Benson, V . Lynch, C . Ouellet, L. Schou, Ti. Stepka, and EJ. E. Tolbert A ~ r i . 1 1 , 1950 Berkeley, California Unclassified D i s t r i b u t i o n No, of Copies Argonne National

  4. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    tuo weeks. For a personal retention copy, call Tech. Info. Division, Ext. 5545 -- - TJNIVERSITY O F CALIFORNIA Radiation Laboratory C ont rae t No, W-74.05-eng-48 THE PATH OF CARBON I N PHOTOSYNTHESIS, X U , KINETIC REIATIORSEIPS OF THE I N T m ~ ~ I A T E S IN sTum STATE PHOTOSYT\JTHESIS A , A. Benson, S . Icawaguchi, F, Hayes and M, Calvfr, Berkeley, Gallfomlh KlMETIC RELATIONSHIPS OF THE INTEiQBDIATLS 3 3 STEADH STATE E'HOTOSY NTHES IS A, A, Benson, So hawaguchf, Po Hayes and M, Calvin

  5. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    UCRL1871 Unclassified-Chemistry D!.stributlon UNIVERSITY OF CALIFORIVIA Radiation Laborator? Contract No. W-7405-eng-48 THE PATH OF CARBON IN PHOTOSYNTHESIS. XVII. PHOSPHORUS COMPOUNDS AS INTERMEDIATES IN PKOTOSYNTHESIS J . G . Buchanan, J. A . Bassham, A. A. Benson, D . F, Bradley, M. Calvin, L . L. Daus, M, Goodman, P . M. Ilaye;8, V . H. Lynch, L. T. N o r r i s a n d A. T. Wilson J u l y 8, 1952 Berkeley, California THE PATH O F CBREiON I N PHCTOSYNTHESIS, XVTI, PHOSPHORUS COMPUUNDS A5 I W I

  6. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    IJCEL-2369 Unclassified - Chemistry D i s t r i b u t i o n UNIVERSITY O F CALIFORML4 Radiation Laboratory Contract No. M-7405- eng - 40 THE P A T H O F CARBON I N PHdGSYNTHESIS .BI. T H E CYCLIC REGENERATION O F CARBON DIOXIDE ACCEPTOR J. A , Bassham, &Ae A. Benson, Lorel D. K r ~ r ~ Anne Z. Harris, A. T. Wilson and M. Calvin October, 1953 Berkeley, California -2- UCRL-2369 Unclassified-Ghemistry Distribution THE P A T H O F CARBON I N P H O T O S Y N T H F S LS , IMI . THE CYCLIC

  7. Damage by X-rays: A Case Study for Metallo-Protein Crystallography

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Damage by X-rays: A Case Study for Metallo-Protein Crystallography Junko Yano1,2, Jan Kern3, Klaus-Dieter Irrgang3, Matthew J. Latimer4, Uwe Bergmann4, Pieter Glatzel5, Yulia Pushkar1,2, Jacek Biesiadka6, Bernhard Loll6, Kenneth Sauer1,2, Johannes Messinger7, Athina Zouni3, Vittal K. Yachandra1 1Melvin Calvin Laboratory, Physical Biosciences Division, Lawrence Berkeley National Laboratory, and 2Department of Chemistry, University of California, Berkeley, CA, USA 3Max-Volmer-Laboratorium für

  8. Chromium Speciation and Mobility in a High Level Nuclear Waste Vadose Zone

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plume 4 John M. Zachara1 Calvin C. Ainsworth,1 Gordon E. Brown, Jr.,2 and Jeffrey G. Catalano2 1Pacific Northwest National Laboratory, Richland, WA 2Stanford University, Stanford, CA Chromate (hexavalent chromium as CrO42-) is a significant groundwater contaminant at the U.S. Department of Energy (DOE) Hanford Site in southeastern WA (Poston et al., 2001) where Pu was produced during WWII and the cold war, and where DOE's largest inventory of legacy wastes remain. Chromate in ground water is

  9. DEPARTMENT OF HEALTH AN~~.N~dtAN, MD. Y.P.H.

    Office of Legacy Management (LM)

    ,' ,...- -., -.- . . we#lnty..: - DEPARTMENT OF HEALTH AN~~.N~dtAN, MD. Y.P.H. April 30, 1979 . _-- _' . U.S.E.P.A. Radiation Branch 26 Federal Plaz;a, Boom 9079 New York, N. Y. 10007 Attention: Miss Feldman:, Gentlemen: In accordance with your request to Calvin E. Weber, P.E., Assistant Commissioner of Health for Environmental Quality, I am forwarding a copy of a report prepared by him concerning a radiation survey conducted in the vicinity of the former Canadian Radium and Uranium Corpora+on

  10. Seating Arrangement, Group Composition and Competition-driven Interaction: Effects on Students' Performance in Physics

    SciTech Connect (OSTI)

    Roxas, R. M.; Monterola, C.; Carreon-Monterola, S. L.

    2010-07-28

    We probe the effect of seating arrangement, group composition and group-based competition on students' performance in Physics using a teaching technique adopted from Mazur's peer instruction method. Ninety eight lectures, involving 2339 students, were conducted across nine learning institutions from February 2006 to June 2009. All the lectures were interspersed with student interaction opportunities (SIO), in which students work in groups to discuss and answer concept tests. Two individual assessments were administered before and after the SIO. The ratio of the post-assessment score to the pre-assessment score and the Hake factor were calculated to establish the improvement in student performance. Using actual assessment results and neural network (NN) modeling, an optimal seating arrangement for a class was determined based on student seating location. The NN model also provided a quantifiable method for sectioning students. Lastly, the study revealed that competition-driven interactions increase within-group cooperation and lead to higher improvement on the students' performance.

  11. Regional economic impacts of changes in electricity rates resulting from Western Area Power Administration`s power marketing alternatives

    SciTech Connect (OSTI)

    Allison, T.; Griffes, P.; Edwards, B.K.

    1995-03-01

    This technical memorandum describes an analysis of regional economic impacts resulting from changes in retail electricity rates due to six power marketing programs proposed by Western Area Power Administration (Western). Regional economic impacts of changes in rates are estimated in terms of five key regional economic variables: population, gross regional product, disposable income, employment, and household income. The REMI (Regional Impact Models, Inc.) and IMPLAN (Impact Analysis for Planning) models simulate economic impacts in nine subregions in the area in which Western power is sold for the years 1993, 2000, and 2008. Estimates show that impacts on aggregate economic activity in any of the subregions or years would be minimal for three reasons. First, the utilities that buy power from Western sell only a relatively small proportion of the total electricity sold in any of the subregions. Second, reliance of Western customers on Western power is fairly low in each subregion. Finally, electricity is not a significant input cost for any industry or for households in any subregion.

  12. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    399 Cy 2 UNIVERSITY OF CALIFORNIA TWO-WEEK LOAN COPY This is a Library Circulating Copy which may be borrowed for two weeks. For a personal retention copy, call Tech. Info. Division, Ext. 5545 BERKELEY, CALIFORNIA UCRL-399 U n c l a s s i f i e d D i s t r i b u t i o n @ 0 - 2 , IJNIIBRSITY O F Ci:LIFOi?WU R a d i a t i o n L a b o r a t o r y C o n t r a c t No. X-7405-eng-48 THE PATH OF CMYOB I M PHOTOSYl!TIIES I S . VII RES PIRoLTI ON AND PHOTOSYNTIiESIS by A, A. Benson and M. Calvin

  13. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    908 UNIVERSITY OF CALIFORNIA TWO-WEEK LOAN COPY This is a Library Circulating Copy which may be borrowed for two weeks. For a personal retention copy, call Tech. Info. Diuision, Ext. 5545 BERKELEY. C A L I F O R N I A UNIVERSITY OF CALIFORNIA R a d i a t i o n L a b o r a t o r y C o n t r a c t No. T-7405-eng-48 THE; PATH OF CARBON I N PHOTOSYNTHESIS, XI THEl ROLE OF GLYCOLIC ACID L . Schou, A,& Benson, J. A, Bassham and Calvin September 11, 1950 Berkeley, C a l i f o r n i a Unclassified D

  14. untitled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    r TWO-WEEK LOAN COPY This is a Library Circulating Copy which may be borrowed for two weeks. For a personal retention copy, call Tech. Info. Diuision, Ext. 5545 U n c l a s s i f i e d - Chemistry UNIVTBSITY OF CALIPOHNU R a d i a t i o n Laboratory Contract No. \?-7405-enp-48 TRE F!YTH OF CARBON I N PHOTOSYNTHESIS. XIV. Melvin Calvin, J. A. B a s s h m , A. A. Renson, S. Kawnyxhi, 7. fi. Lynch, ! i . Stepka, arid N. E . T o l k e r t . June ?0, 1951 Berkeley, C a l i f o r n i a It seems hardly

  15. Temperature-package power correlations for open-mode geologic disposal concepts.

    SciTech Connect (OSTI)

    Hardin, Ernest L.

    2013-02-01

    Logistical simulation of spent nuclear fuel (SNF) management in the U.S. combines storage, transportation and disposal elements to evaluate schedule, cost and other resources needed for all major operations leading to final geologic disposal. Geologic repository reference options are associated with limits on waste package thermal power output at emplacement, in order to meet limits on peak temperature for certain key engineered and natural barriers. These package power limits are used in logistical simulation software such as CALVIN, as threshold requirements that must be met by means of decay storage or SNF blending in waste packages, before emplacement in a repository. Geologic repository reference options include enclosed modes developed for crystalline rock, clay or shale, and salt. In addition, a further need has been addressed for open modes in which SNF can be emplaced in a repository, then ventilated for decades or longer to remove heat, prior to permanent repository closure. For each open mode disposal concept there are specified durations for surface decay storage (prior to emplacement), repository ventilation, and repository closure operations. This study simulates those steps for several timing cases, and for SNF with three fuel-burnup characteristics, to develop package power limits at which waste packages can be emplaced without exceeding specified temperature limits many years later after permanent closure. The results are presented in the form of correlations that span a range of package power and peak postclosure temperature, for each open-mode disposal concept, and for each timing case. Given a particular temperature limit value, the corresponding package power limit for each case can be selected for use in CALVIN and similar tools.

  16. Targeted Enhancement of H2 and CO2 Uptake for Autotrophic Production of Biodiesel in the Lithoautotrophic Bacterium Ralsonia Eutropha

    SciTech Connect (OSTI)

    Eckert, C. A.; Sullivan, R.; Johnson, C.; Yu, J.; Maness, P. C.

    2013-01-01

    CO2 and H2 are promising feedstocks for production of valuable biocompounds. Ralstonia eutropha utilizes these feedstocks to generate energy (ATP) and reductant (NAD(P)H) via oxidation of H2 by a membrane-bound (MBH) and a soluble hydrogenase (SH) for CO2 fixation by the Calvin-Benson-Bassham (CBB) cycle. Increased expression of the enzyme that fixes CO2 (RubisCO) resulted in 6-fold activity improvement in vitro, while increased expression of the MBH operon or the SH operon plus MBH operon maturation factors necessary for activity resulted in a 10-fold enhancement. Current research involves genetic manipulation of two endogenous cbb operons for increased expression, analysis of expression and activity of CBB/MBH/SH, cofactor ratios, and downstream products during autotrophic growth in control versus enhanced strains, and development of strategies for long-term, optimal overexpression. These studies will improve our understanding of autotrophic metabolism and provide a chassis strain for autotrophic production of biodiesel and other valuable carbon biocompounds.

  17. Enhancement of CO2 and H2 Uptake for the Production of Biodiesel in Cupriavidus Necator

    SciTech Connect (OSTI)

    Sullivan, R. P.; Eckert, C. A.; Balzer, G. J.; Yu, J.; Maness, P. C.

    2012-01-01

    Cupriavidus necator fixes CO{sub 2} through the Calvin-Benson-Bassham (CBB) cycle using electrons and energy obtained from the oxidation of H{sub 2}. Producing biodiesel-equivalent electrofuel from renewable CO{sub 2} and H{sub 2} has immense potential, especially if the fuel is compatible with the existing fuel infrastructure. This research addressed enhanced substrate utilization by focusing on two strategies: (1) optimizing transcriptional regulations to afford over-expression of Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the enzyme responsible for assimilation of CO{sub 2} into the CBB cycle; and (2) hydrogenase over-expression by introduction of additional copies of genes encoding a membrane-bound hydrogenase (MBH), a soluble hydrogenase (SH), and their maturation machinery to enhance oxidation of H{sub 2} to generate NAD(P)H and ATP required for CO{sub 2} fixation. Incorporation of these strategies into a single production strain resulted in 6-fold CO{sub 2} and 3-fold H{sub 2} uptake improvement, in vitro, with the overarching goal of providing abundant reducing equivalents towards the economic production of biodiesel in C. necator.

  18. Energy Efficient Buildings, Salt Lake County, Utah

    SciTech Connect (OSTI)

    Barnett, Kimberly

    2012-04-30

    Executive Summary Salt Lake County's Solar Photovoltaic Project - an unprecedented public/private partnership Salt Lake County is pleased to announce the completion of its unprecedented solar photovoltaic (PV) installation on the Calvin R. Rampton Salt Palace Convention Center. This 1.65 MW installation will be one the largest solar roof top installations in the country and will more than double the current installed solar capacity in the state of Utah. Construction is complete and the system will be operational in May 2012. The County has accomplished this project using a Power Purchase Agreement (PPA) financing model. In a PPA model a third-party solar developer will finance, develop, own, operate, and maintain the solar array. Salt Lake County will lease its roof, and purchase the power from this third-party under a long-term Power Purchase Agreement contract. In fact, this will be one of the first projects in the state of Utah to take advantage of the recent (March 2010) legislation which makes PPA models possible for projects of this type. In addition to utilizing a PPA, this solar project will employ public and private capital, Energy Efficiency and Conservation Block Grants (EECBG), and public/private subsidized bonds that are able to work together efficiently because of the recent stimulus bill. The project also makes use of recent changes to federal tax rules, and the recent re-awakening of private capital markets that make a significant public-private partnership possible. This is an extremely innovative project, and will mark the first time that all of these incentives (EECBG grants, Qualified Energy Conservation Bonds, New Markets tax credits, investment tax credits, public and private funds) have been packaged into one project. All of Salt Lake County's research documents and studies, agreements, and technical information is available to the public. In addition, the County has already shared a variety of information with the public through webinars, site tours, presentations, and written correspondence.

  19. Engineering Ralstonia eutropha for Production of Isobutanol (IBT) Motor Fuel from Carbon Dioxide, Hydrogen, and Oxygen Project Final Report

    SciTech Connect (OSTI)

    Sinskey, Anthony J.; Worden, Robert Mark; Brigham, Christopher; Lu, Jingnan; Quimby, John Westlake; Gai, Claudia; Speth, Daan; Elliott, Sean; Fei, John Qiang; Bernardi, Amanda; Li, Sophia; Grunwald, Stephan; Grousseau, Estelle; Maiti, Soumen; Liu, Chole

    2013-12-16

    This research project is a collaboration between the Sinskey laboratory at MIT and the Worden laboratory at Michigan State University. The goal of the project is to produce Isobutanol (IBT), a branched-chain alcohol that can serve as a drop-in transportation fuel, through the engineered microbial biosynthesis of Carbon Dioxide, Hydrogen, and Oxygen using a novel bioreactor. This final technical report presents the findings of both the biological engineering work at MIT that extended the native branched-chain amino acid pathway of the wild type Ralstonia eutropha H16 to perform this biosynthesis, as well as the unique design, modeling, and construction of a bioreactor for incompatible gasses at Michigan State that enabled the operational testing of the complete system. This 105 page technical report summarizing the three years of research includes 72 figures and 11 tables of findings. Ralstonia eutropha (also known as Cupriavidus necator) is a Gram-negative, facultatively chemolithoautotrophic bacteria. It has been the principle organism used for the study of polyhydroxybutyrate (PHB) polymer biosynthesis. The wild-type Ralstonia eutropha H16 produces PHB as an intracellular carbon storage material while under nutrient stress in the presence of excess carbon. Under this stress, it can accumulate approximately 80 % of its cell dry weight (CDW) as this intracellular polymer. With the restoration of the required nutrients, the cells are then able to catabolize this polymer. If extracted from the cell, this PHB polymer can be processed into biodegradable and biocompatible plastics, however for this research, it is the efficient metabolic pathway channeling the captured carbon that is of interest. R. eutropha is further unique in that it contains two carbon-fixation CalvinBensonBassham cycle operons, two oxygen-tolerant hydrogenases, and several formate dehydrogenases. It has also been much studied for its ability in the presence of oxygen, to fix carbon dioxide into complex cellular molecules using the energy from hydrogen. In this research project, engineered strains of R. eutropha redirected the excess carbon from PHB storage into the production of isobutanol and 3-methyl-1-butanol (branched-chain higher alcohols). These branched-chain higher alcohols can be used directly as substitutes for fossil-based fuels and are seen as alternative biofuels to ethanol and biodiesel. Importantly, these alcohols have approximately 98 % of the energy content of gasoline, 17 % higher than the current gasoline additive ethanol, without impacting corn market production for feed or food. Unlike ethanol, these branched-chain alcohols have low vapor pressure, hygroscopicity, and water solubility, which make them readily compatible with the existing pipelines, gasoline pumps, and engines in our transportation infrastructure. While the use of alternative energies from solar, wind, geothermal, and hydroelectric has spread for stationary power applications, these energy sources cannot be effectively or efficiently employed in current or future transportation systems. With the ongoing concerns of fossil fuel availability and price stability over the long term, alternative biofuels like branched-chain higher alcohols hold promise as a suitable transportation fuel in the future. We showed in our research that various mutant strains of R. eutropha with isobutyraldehyde dehydrogenase activity, in combination with the overexpression of plasmid-borne, native branched-chain amino acid biosynthesis pathway genes and the overexpression of heterologous ketoisovalerate decarboxylase gene, would produce isobutanol and 3-methyl-1-butanol when initiated during nitrogen or phosphorus limitation. Early on, we isolated one mutant R. eutropha strain which produced over 180 mg/L branched-chain alcohols in flask culture while being more tolerant of isobutanol toxicity. After the targeted elimination of genes encoding several potential carbon sinks (ilvE, bkdAB, and aceE), the production titer of the improved to 270 mg/L isobutanol and 40 mg/L 3-methyl-1-butanol.

  20. Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    James C. Liao

    2012-05-22

    This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.