National Library of Energy BETA

Sample records for haiti hondu ras

  1. Haiti-IAEA Energy Planning | Open Energy Information

    Open Energy Info (EERE)

    Haiti1 IAEA is working with Haiti to strengthen the management and development of energy sources. References "IAEA Project database- Haiti" Retrieved from "http:...

  2. Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap...

    Open Energy Info (EERE)

    Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap and Strategy Jump to: navigation, search Name Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap and...

  3. Haiti-Pilot Program for Climate Resilience (PPCR) | Open Energy...

    Open Energy Info (EERE)

    Pilot Program for Climate Resilience (PPCR) Jump to: navigation, search Name Haiti-Pilot Program for Climate Resilience (PPCR) AgencyCompany Organization World Bank Sector...

  4. K-Ras4B proteins are expressed in the nucleolus: Interaction with nucleolin

    SciTech Connect (OSTI)

    Birchenall-Roberts, Maria C. . E-mail: birchena@mail.ncifcrf.gov; Fu, Tao; Kim, Soo-Gyung; Huang, Ying K.; Dambach, Michael; Resau, James H.; Ruscetti, Francis W.

    2006-09-22

    Kirsten Ras4B (K-Ras4B) is a potent onco-protein that is expressed in the majority of human cell types and is frequently mutated in carcinomas. K-Ras4B, like other members of the Ras family of proteins, is considered to be a cytoplasmic protein that must be localized to the plasma membrane for activation. Here, using confocal microscopy and biochemical analysis, we show that K-Ras4B, but not H-Ras or the closely related K-Ras4A, is also present in the nucleoli of normal and transformed cells. Subcellular fractionation and immunostaining show that K-Ras4B is located not only in the cytoplasm, but also in the nucleolar compartment. Modification of a C-terminal hexa-lysine motif unique to K-Ras4B results in exclusively cytoplasmic forms of the protein. Nucleolin, a pleiotropic regulator of cellular processes, including transcriptional regulation, is also characterized by a nucleolar-like nuclear appearance. We show that K-Ras4B and nucleolin co-localize within the nucleus and that nucleolin physically associates with K-Ras4B. Inhibition of K-Ras4B/nucleolin association blocked nucleolar localization of K-Ras4B. Using siRNA to knockdown the expression of nucleolin eliminated the nucleolar localization of K-Ras4B and significantly repressed the activation of the well-characterized K-Ras4B transcriptional target Ap-1, but stimulated Elk1. These data provide evidence of a nucleolar localization of K-Ras4B and describe a functional association between K-Ras4B and nucleolin.

  5. Performance of Charcoal Cookstoves for Haiti, Part 2: Results from the Controlled Cooking Test

    SciTech Connect (OSTI)

    Lask, Kathleen; Jones, Jennifer; Booker, Kayje; Ceballos, Cristina; Yang, Nina; Gadgil, Ashok

    2011-11-30

    Five charcoal cookstoves were tested using a Controlled Cooking Test (CCT) developed from cooking practices in Haiti. Cookstoves were tested for total burn time, specific fuel consumption, and emissions of carbon monoxide (CO), carbon dioxide (CO{sub 2}), and the ratio of carbon monoxide to carbon dioxide (CO/CO{sub 2}). These results are presented in this report along with LBNL testers’ observations regarding the usability of the stoves.

  6. Haiti: Feasibility of Waste-to-Energy Options at the Trutier Waste Site

    SciTech Connect (OSTI)

    Conrad, M. D.; Hunsberger, R.; Ness, J. E.; Harris, T.; Raibley, T.; Ursillo, P.

    2014-08-01

    This report provides further analysis of the feasibility of a waste-to-energy (WTE) facility in the area near Port-au-Prince, Haiti. NREL's previous analysis and reports identified anaerobic digestion (AD) as the optimal WTE technology at the facility. Building on the prior analyses, this report evaluates the conceptual financial and technical viability of implementing a combined waste management and electrical power production strategy by constructing a WTE facility at the existing Trutier waste site north of Port-au-Prince.

  7. Ras-GTP dimers activate the mitogen-activated protein kinase (MAPK) pathway

    SciTech Connect (OSTI)

    Nan, Xiaolin; Tamgney, Tanja M.; Collisson, Eric A.; Lin, Li -Jung; Pitt, Cameron; Galeas, Jacqueline; Lewis, Sophia; Gray, Joe W.; McCormick, Frank; Chu, Steven

    2015-06-16

    Rat sarcoma (Ras) GTPases regulate cell proliferation and survival through effector pathways including Raf-MAPK, and are the most frequently mutated genes in human cancer. Although it is well established that Ras activity requires binding to both GTP and the membrane, details of how Ras operates on the cell membrane to activate its effectors remain elusive. Efforts to target mutant Ras in human cancers to therapeutic benefit have also been largely unsuccessful. Here we show that Ras-GTP forms dimers to activate MAPK. We used quantitative photoactivated localization microscopy (PALM) to analyze the nanoscale spatial organization of PAmCherry1-tagged KRas 4B (hereafter referred to KRas) on the cell membrane under various signaling conditions. We found that at endogenous expression levels KRas forms dimers, and KRasG12D, a mutant that constitutively binds GTP, activates MAPK. Overexpression of KRas leads to formation of higher order Ras nanoclusters. Conversely, at lower expression levels, KRasG12D is monomeric and activates MAPK only when artificially dimerized. Moreover, dimerization and signaling of KRas are both dependent on an intact CAAX (C, cysteine; A, aliphatic; X, any amino acid) motif that is also known to mediate membrane localization. These results reveal a new, dimerization-dependent signaling mechanism of Ras, and suggest Ras dimers as a potential therapeutic target in mutant Ras-driven tumors.

  8. Ras-GTP dimers activate the mitogen-activated protein kinase (MAPK) pathway

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nan, Xiaolin; Tamgüney, Tanja M.; Collisson, Eric A.; Lin, Li -Jung; Pitt, Cameron; Galeas, Jacqueline; Lewis, Sophia; Gray, Joe W.; McCormick, Frank; Chu, Steven

    2015-06-16

    Rat sarcoma (Ras) GTPases regulate cell proliferation and survival through effector pathways including Raf-MAPK, and are the most frequently mutated genes in human cancer. Although it is well established that Ras activity requires binding to both GTP and the membrane, details of how Ras operates on the cell membrane to activate its effectors remain elusive. Efforts to target mutant Ras in human cancers to therapeutic benefit have also been largely unsuccessful. Here we show that Ras-GTP forms dimers to activate MAPK. We used quantitative photoactivated localization microscopy (PALM) to analyze the nanoscale spatial organization of PAmCherry1-tagged KRas 4B (hereafter referredmore » to KRas) on the cell membrane under various signaling conditions. We found that at endogenous expression levels KRas forms dimers, and KRasG12D, a mutant that constitutively binds GTP, activates MAPK. Overexpression of KRas leads to formation of higher order Ras nanoclusters. Conversely, at lower expression levels, KRasG12D is monomeric and activates MAPK only when artificially dimerized. Moreover, dimerization and signaling of KRas are both dependent on an intact CAAX (C, cysteine; A, aliphatic; X, any amino acid) motif that is also known to mediate membrane localization. These results reveal a new, dimerization-dependent signaling mechanism of Ras, and suggest Ras dimers as a potential therapeutic target in mutant Ras-driven tumors.« less

  9. Evaluation of Potential to Air Ingestion From RWT Following RAS

    SciTech Connect (OSTI)

    Bang, Young S.; Kim, Ingoo; Woo, Sweng W.

    2006-07-01

    At the Recirculation Actuation Signal (RAS) when the Refueling Water Tank (RWT) water level decreased to a certain value following Loss-of-Coolant Accident (LOCA), the isolation valves of Containment Recirculation Sump (CRS) of the Korean Standard Nuclear Power Plants (KSNP) are open automatically while the RWT isolation valves would be closed manually. It was concerned whether the design has a potential to air ingestion to Emergency Core Cooling System (ECCS) pumps before completion of the manual action to close RWT isolation valves. To support the safety evaluation on this issue including the evaluation of design adequacy, an analysis of the hydraulic transient within the ECCS piping following the RAS in KSNP is performed. RELAP5/MOD3.3 code is used to calculate the transient behavior of the piping network. The code was known to have capability to calculate one-dimensional two-phase transient flow with noncondensable gas in the complex piping. Substantial portion of ECCS are modeled including RWT, CRS, each pipe line from RWT and CRS to connection point with its own isolation valve and check valve, a common pipe line to ECCS header, each pipe line from the header to High Pressure Safety Injection (HPSI) pump, Low Pressure Safety Injection (LPSI) pump, and Containment Spray (CS) pump. Transient hydraulic behavior in the piping system following RAS after LOCA is calculated. It is found that the RWT water level was always higher than the elevation of the check valve at the connecting point by more than 15 ft. It indicates the air intrusion to the check valve can be sufficiently prevented by this amount of water head. (authors)

  10. Ras Al Khaimah (RAK): World Oil Report 1991

    SciTech Connect (OSTI)

    Not Available

    1991-08-01

    This paper reports on Ras Al Khalmah (RAK) that had no drilling activity in 1990. In 1991, International Petroleum Ltd., (IPL), which holds a 1,100 sq mi concession onshore, may spud their West Jiri prospect. Amoco holds an offshore concession and was to have completed seismic last year. the RAK Gas Commission was reported to be operating the offshore Saleh gas field in 1990, which a former foreign concessionaire relinquished in mid-1989 since it had reached its economic limit. Production from Saleh last year is estimated at 1,000 bcpd and about 9 MMcfgd.

  11. Energy Transition Initiative: Island Energy Snapshot - Haiti; U.S. Department of Energy (DOE), NREL (National Renewable Energy Laboratory)

    SciTech Connect (OSTI)

    2015-06-01

    This profile provides a snapshot of the energy landscape of Haiti, an independent nation that occupies the western portion of the island of Hispaniola in the northern Caribbean Sea. Haiti’s utility rates are roughly $0.35 U.S. dollars (USD) per kilowatt-hour (kWh), above the Caribbean regional average of $0.33 USD/kWh.

  12. Characterization of a Ras Mutant with Identical GDP- and GTP-Bound Structures

    SciTech Connect (OSTI)

    Ford, B.; Boykevisch, S; Zhao, C; Kunzelmann, S; Bar-Sagi, D; Herrmann, C; Nassar, N

    2009-01-01

    We previously characterized the G60A mutant of Ras and showed that the switch regions of the GTP-bound but not the GDP-bound form of this mutant adopt an 'open conformation' similar to that seen in nucleotide-free Ras. Here, we mutate Lys147 of the conserved {sup 145}SAK{sup 147} motif in the G60A background and characterize the resulting double mutant (DM). We show that RasDM is the first structure of a Ras protein with identical GDP- and GTP-bound structures. Both structures adopt the open conformation of the active form of RasG60A. The increase in the accessible surface area of the nucleotide is consistent with a 4-fold increase in its dissociation rate. Stopped-flow experiments show no major difference in the two-step kinetics of association of GDP or GTP with the wild type, G60A, or RasDM. Addition of Sos fails to accelerate nucleotide exchange. Overexpression of the G60A or double mutant of Ras in COS-1 cells fails to activate Erk and shows a strong dominant negative effect. Our data suggest that flexibility at position 60 is required for proper Sos-catalyzed nucleotide exchange and that structural information is somehow shared among the switch regions and the different nucleotide binding motifs.

  13. Structure of the Dominant Negative S17N Mutant of Ras

    SciTech Connect (OSTI)

    Nassar, N.; Singh, K; Garcia-Diaz, M

    2010-01-01

    The use of the dominant negative mutant of Ras has been crucial in elucidating the cellular signaling of Ras in response to the activation of various membrane-bound receptors. Although several point mutants of Ras exhibit a dominant negative effect, the asparagine to serine mutation at position 17 (S17N) remains the most popular and the most effective at inhibiting the activation of endogenous Ras. It is now widely accepted that the dominant negative effect is due to the ability of the mutant to sequester upstream activators and its inability to activate downstream effectors. Here, we present the crystal structure of RasS17N in the GDP-bound form. In the three molecules that populate the asymmetric unit, the Mg{sup 2+} ion that normally coordinates the {beta}-phosphate is absent because of steric hindrance from the Asn17 side chain. Instead, a Ca{sup 2+} ion is coordinating the {alpha}-phosphate. Also absent from one molecule is electron density for Phe28, a conserved residue that normally stabilizes the nucleotide's guanine base. Except for Phe28, the nucleotide makes conserved interactions with Ras. Combined, the inability of Phe28 to stabilize the guanine base and the absence of a Mg{sup 2+} ion to neutralize the negative charges on the phosphates explain the weaker affinity of GDP for Ras. Our data suggest that the absence of the Mg{sup 2+} should also dramatically affect GTP binding to Ras and the proper positioning of Thr35 necessary for the activation of switch 1 and the binding to downstream effectors, a prerequisite for the triggering of signaling pathways.

  14. Performance of Charcoal Cookstoves for Haiti Part 1: Results from the Water Boiling Test

    SciTech Connect (OSTI)

    Booker, Kayje; Han, Tae Won; Granderson, Jessica; Jones, Jennifer; Lsk, Kathleen; Yang, Nina; Gadgil, Ashok

    2011-06-01

    In April 2010, a team of scientists and engineers from Lawrence Berkeley National Lab (LBNL) and UC Berkeley, with support from the Darfur Stoves Project (DSP), undertook a fact-finding mission to Haiti in order to assess needs and opportunities for cookstove intervention. Based on data collected from informal interviews with Haitians and NGOs, the team, Scott Sadlon, Robert Cheng, and Kayje Booker, identified and recommended stove testing and comparison as a high priority need that could be filled by LBNL. In response to that recommendation, five charcoal stoves were tested at the LBNL stove testing facility using a modified form of version 3 of the Shell Foundation Household Energy Project Water Boiling Test (WBT). The original protocol is available online. Stoves were tested for time to boil, thermal efficiency, specific fuel consumption, and emissions of CO, CO{sub 2}, and the ratio of CO/CO{sub 2}. In addition, Haitian user feedback and field observations over a subset of the stoves were combined with the experiences of the laboratory testing technicians to evaluate the usability of the stoves and their appropriateness for Haitian cooking. The laboratory results from emissions and efficiency testing and conclusions regarding usability of the stoves are presented in this report.

  15. Qatargas exporting LNG from Qatar`s new Ras Laffan Port

    SciTech Connect (OSTI)

    1997-02-24

    When the 135,000 cu m LNG carrier Al Zubarah departed Ras Laffan Port in December, Qatar entered a new era of commerce that will both boost the emirate`s economic development and influence energy trade around the world. The event capped more than a decade of planning, design, and construction of Ras Laffan Port--the world`s newest and largest LNG exporting facility. During the 1980s, the focus in Qatar was on exploration and development of North field, which holds the world`s largest reserves of nonassociated natural gas. In the 1990s, efforts concentrated on establishing a direct production and export link between North field, the new multi-billion-dollar Qatar Liquefied Gas Co. (Qatargas) gas liquefaction plant at Ras Laffan, and LNG export facilities at the 8.5 sq km Ras Laffan Port. Markets of the Far East will be first to be served by LNG from Ras Laffan Port. Two 25-year LNG supply contracts have been signed with buyers in Japan and South Korea, and negotiations are under way with potential customers from China, Taiwan, and Thailand. The paper describes the port, its operations, and export projects.

  16. Novel approach to abuse the hyperactive K-Ras pathway for adenoviral gene therapy of colorectal cancer

    SciTech Connect (OSTI)

    Naumov, Inna; Kazanov, Dina; Lisiansky, Victoria; Starr, Alex; Aroch, Ilan; Shapira, Shiran; Kraus, Sarah; Arber, Nadir

    2012-01-15

    Background: Functional activation of oncogenic K-Ras signaling pathway plays an important role in the early events of colorectal carcinogenesis (CRC). K-Ras proto-oncogene is involved in 35-40% of CRC cases. Mutations in the Ras gene trigger the transduction of proliferative and anti-apoptotic signals, even in the absence of extra cellular stimuli. The objective of the current study was to use a gene-targeting approach to kill human CRC cells selectively harboring mutated K-Ras. Results: A recombinant adenovirus that carries a lethal gene, PUMA, under the control of a Ras responsive promoter (Ad-Py4-SV40-PUMA) was used selectively to target CRC cells (HCT116, SW480, DLD1 and RIE-Ras) that possess a hyperactive Ras pathway while using HT29 and RIE cells as a control that harbors wild type Ras and exhibit very low Ras activity. Control vector, without the Ras responsive promoter elements was used to assess the specificity of our 'gene therapy' approach. Both adenoviral vectors were assed in vitro and in xenograft model in vivo. Ad-Py4-SV40-PUMA showed high potency to induce {approx} 50% apoptosis in vitro, to abolish completely tumor formation by infecting cells with the Ad-Py4-SV40-PUMA prior xenografting them in nude mice and high ability to suppress by {approx} 35% tumor progression in vivo in already established tumors. Conclusions: Selective targeting of CRC cells with the activated Ras pathway may be a novel and effective therapy in CRC. The high potency of this adenoviral vector may help to overcome an undetectable micro metastasis that is the major hurdle in challenging with CRC.

  17. Activation of RAS/ERK alone is insufficient to inhibit RXRα function and deplete retinoic acid in hepatocytes

    SciTech Connect (OSTI)

    Wang, Ai-Guo Song, Ya-Nan; Chen, Jun; Li, Hui-Ling; Dong, Jian-Yi; Cui, Hai-Peng; Yao, Liang; Li, Xue-Feng; Gao, Wen-Ting; Qiu, Ze-Wen; Wang, Fu-Jin; Wang, Jing-Yu

    2014-09-26

    Highlights: • The activation of RAS/ERK is insufficient to inhibit RXRα function and deplete RA. • The retinoid metabolism-related genes are down-regulated by ras oncogene. • The atRA has no effect on preventing hepatic tumorigenesis or curing the developed hepatic nodules. - Abstract: Activation of RAS/ERK signaling pathway, depletion of retinoid, and phosphorylation of retinoid X receptor alpha (RXRα) are frequent events found in liver tumors and thought to play important roles in hepatic tumorigenesis. However, the relationships among them still remained to be elucidated. By exploring the transgenic mouse model of hepatic tumorigenesis induced by liver-specific expression of H-ras12V oncogene, the activation of RAS/ERK, the mRNA expression levels of retinoid metabolism-related genes, the contents of retinoid metabolites, and phosphorylation of RXRα were determined. RAS/ERK signaling pathway was gradually and significantly activated in hepatic tumor adjacent normal liver tissues (P) and hepatic tumor tissues (T) of H-ras12V transgenic mice compared with normal liver tissues (Wt) of wild type mice. On the contrary, the mRNA expression levels of retinoid metabolism-related genes were significantly reduced in P and T compared with Wt. Interestingly, the retinoid metabolites 9-cis-retinoic acid (9cRA) and all-trans-retinoic acid (atRA), the well known ligands for nuclear transcription factor RXR and retinoic acid receptor (RAR), were significantly decreased only in T compared with Wt and P, although the oxidized polar metabolite of atRA, 4-keto-all-trans-retinoic-acid (4-keto-RA) was significantly decreased in both P and T compared with Wt. To our surprise, the functions of RXRα were significantly blocked only in T compared with Wt and P. Namely, the total protein levels of RXRα were significantly reduced and the phosphorylation levels of RXRα were significantly increased only in T compared with Wt and P. Treatment of H-ras12V transgenic mice at 5-week

  18. Senescence-Associated Secretory Phenotypes Reveal Cell-Nonautonomous Functions of Oncogenic RAS and the p53 Tumor Suppressor

    SciTech Connect (OSTI)

    Coppé, Jean-Philippe; Patil, Christopher; Rodier, Francis; Sun, Yu; Munoz, Denise; Goldstein, Joshua; Nelson, Peter; Desprez, Pierre-Yves; Campisi, Judith

    2008-10-24

    Cellular senescence suppresses cancer by arresting cell proliferation, essentially permanently, in response to oncogenic stimuli, including genotoxic stress. We modified the use of antibody arrays to provide a quantitative assessment of factors secreted by senescent cells. We show that human cells induced to senesce by genotoxic stress secrete myriad factors associated with inflammation and malignancy. This senescence-associated secretory phenotype (SASP) developed slowly over several days and only after DNA damage of sufficient magnitude to induce senescence. Remarkably similar SASPs developed in normal fibroblasts, normal epithelial cells, and epithelial tumor cells after genotoxic stress in culture, and in epithelial tumor cells in vivo after treatment of prostate cancer patients with DNA-damaging chemotherapy. In cultured premalignant epithelial cells, SASPs induced an epithelial-mesenchyme transition and invasiveness, hallmarks of malignancy, by a paracrine mechanism that depended largely on the SASP factors interleukin (IL)-6 and IL-8. Strikingly, two manipulations markedly amplified, and accelerated development of, the SASPs: oncogenic RAS expression, which causes genotoxic stress and senescence in normal cells, and functional loss of the p53 tumor suppressor protein. Both loss of p53 and gain of oncogenic RAS also exacerbated the promalignant paracrine activities of the SASPs. Our findings define a central feature of genotoxic stress-induced senescence. Moreover, they suggest a cell-nonautonomous mechanism by which p53 can restrain, and oncogenic RAS can promote, the development of age-related cancer by altering the tissue microenvironment.

  19. Reduction of metastasis, cell invasion, and adhesion in mouse osteosarcoma by YM529/ONO-5920-induced blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathway

    SciTech Connect (OSTI)

    Tsubaki, Masanobu; Satou, Takao; Itoh, Tatsuki; Imano, Motohiro; Ogaki, Mitsuhiko; Yanae, Masashi; Depeartment of Pharmacy, Sakai Hospital, Kinki University School of Medicine, Sakai, Osaka 590-0132 ; Nishida, Shozo

    2012-03-15

    Osteosarcoma is one of the most common primary malignant bone tumors in children and adolescents. Some patients continue to have a poor prognosis, because of the metastatic disease. YM529/ONO-5920 is a nitrogen-containing bisphosphonate that has been used for the treatment of osteoporosis. YM529/ONO-5920 has recently been reported to induce apoptosis in various tumors including osteosarcoma. However, the mode of metastasis suppression in osteosarcoma by YM529/ONO-5920 is unclear. In the present study, we investigated whether YM529/ONO-5920 inhibited tumor cell migration, invasion, adhesion, or metastasis in the LM8 mouse osteosarcoma cell line. We found that YM529/ONO-5920 significantly inhibited metastasis, cell migration, invasion, and adhesion at concentrations that did not have antiproliferative effects on LM8 cells. YM529/ONO-5920 also inhibited the mRNA expression and protein activities of matrix metalloproteinases (MMPs). In addition, YM529/ONO-5920 suppressed phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and the serine/threonine protein kinase B (Akt) by the inhibition of Ras prenylation. Moreover, U0126, a mitogen-activated protein kinase kinase (MEK) 1/2 inhibitor, and LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, also inhibited LM8 cell migration, invasion, adhesion, and metastasis, as well as the mRNA expression and protein activities of MMP-1, MMP-2, MMP-9, and MT1-MMP. The results indicated that YM529/ONO-5920 suppressed the Ras/MEK/ERK and Ras/PI3K/Akt pathways, thereby inhibiting LM8 cell migration, invasion, adhesion, and metastasis. These findings suggest that YM529/ONO-5920 has potential clinical applications for the treatment of tumor cell metastasis in osteosarcoma. -- Highlights: ? We investigated whether YM529/ONO-5920 inhibited tumor metastasis in osteosarcoma. ? YM529/ONO-5920 inhibited metastasis, cell migration, invasion, and adhesion. ? YM529/ONO-5920 suppressed Ras signalings. ? YM529/ONO-5920 has

  20. Shift in the Equilibrium between On and Off States of the Allosteric Switch in Ras-GppNHp Affected by Small Molecules and Bulk Solvent Composition

    SciTech Connect (OSTI)

    Holzapfel, Genevieve; Buhrman, Greg; Mattos, Carla

    2012-08-31

    Ras GTPase cycles between its active GTP-bound form promoted by GEFs and its inactive GDP-bound form promoted by GAPs to affect the control of various cellular functions. It is becoming increasingly apparent that subtle regulation of the GTP-bound active state may occur through promotion of substates mediated by an allosteric switch mechanism that induces a disorder to order transition in switch II upon ligand binding at an allosteric site. We show with high-resolution structures that calcium acetate and either dithioerythritol (DTE) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence of a moderate amount of poly(ethylene glycol) (PEG) can selectively shift the equilibrium to the 'on' state, where the active site appears to be poised for catalysis (calcium acetate), or to what we call the 'ordered off' state, which is associated with an anticatalytic conformation (DTE or DTT). We also show that the equilibrium is reversible in our crystals and dependent on the nature of the small molecule present. Calcium acetate binding in the allosteric site stabilizes the conformation observed in the H-Ras-GppNHp/NOR1A complex, and PEG, DTE, and DTT stabilize the anticatalytic conformation observed in the complex between the Ras homologue Ran and Importin-{beta}. The small molecules are therefore selecting biologically relevant conformations in the crystal that are sampled by the disordered switch II in the uncomplexed GTP-bound form of H-Ras. In the presence of a large amount of PEG, the ordered off conformation predominates, whereas in solution, in the absence of PEG, switch regions appear to remain disordered in what we call the off state, unable to bind DTE.

  1. Haiti earthquake survivor to speak

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science Laboratory Auditorium and is open to Laboratory badge holders and escorted media. Members of the news media who wish to attend the talk should contact the LANL...

  2. Fibroblast growth factor-2 up-regulates the expression of nestin through the RasRafERKSp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: Nestin expression in C6 glioma cells is induced by FGF-2. Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. The mRNA of FGFR1 and 3 are detected in C6 glioma cells. RasRafERKSp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFRMAPKERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  3. Haiti-NREL Cooperation | Open Energy Information

    Open Energy Info (EERE)

    Publics, Transports et Communications (Ministry of Public Works, Transportation and Communication). References "NREL International Program" Retrieved from "http:...

  4. Haiti: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    lack of investment in the energy infrastructure, caused in part by the fact that prices are artificially held below the true cost of production and delivery. Power supply is...

  5. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  6. Haiti-Designing and Communicating Low Carbon Energy Roadmaps...

    Open Energy Info (EERE)

    Partner International Climate Initiative Sector Climate, Energy Focus Area Renewable Energy, Buildings, Economic Development, Energy Efficiency, Greenhouse Gas, Grid Assessment...

  7. Haiti-Facility for Environmentally Friendly Transport Technology...

    Open Energy Info (EERE)

    entitled Navigating Transport NAMAs, which is tailored to each target group, outlines the instruments and technologies available as well as the context for climate negotiations...

  8. Haiti - Annual Average Wind Speed at 80 meters

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Liberte Hinche 06-JAN-2014 3.5.1 50 0 Port-au-Prince Jacmel Les Cayes Jeremie 50 100 Kilometers DOMINI REPUBL CAN IC The wind resource estimates on this map are from model...

  9. Haiti-Low-Carbon Energy Roadmaps for the Greater Antilles | Open...

    Open Energy Info (EERE)

    Institute Partner Deutsche Gesellschaft fr Internationale Zusammenarbeit (GIZ) GmbH, National Energy Commission Sector Climate, Energy Focus Area Economic Development,...

  10. Gateway:Amrica Latina | Open Energy Information

    Open Energy Info (EERE)

    Haiti Haiti Honduras Honduras Mexico Mexico Nicaragua Nicaragua Panama Panama Paraguay Paraguay Peru Peru Republica Dominicana Dominican Republic Uruguay Uruguay Venezuela...

  11. CX-008556: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Haiti Renewable Resource Study CX(s) Applied: A9, A11 Date: 07/23/2012 Location(s): Haiti Offices(s): Golden Field Office

  12. OAiC RiDGE NATIONAL LABORAl-ORY LKCKKBSP HAITI MANA%ED AND OPERATED...

    Office of Legacy Management (LM)

    ... of the former ACL building and the residential property to the northeast . . . . . . . ... Restoration, U.S. Department of Energy, under contract DE-ACO5960R22464 with ...

  13. Reactive amendment saltstone (RAS). A novel approach for improved sorption/retention of radionuclides such as technetium and iodine

    SciTech Connect (OSTI)

    Dixon, K. L.; Knox, A. S.; Cozzi, A. D.; Flach, G. P.; Hill, K. A.

    2015-09-30

    This study examined the use of reactive amendments (hydroxyapatite, activated carbon, and two types of organoclays) that prior research suggests may improve retention of 99Tc and 129I. Tests were conducted using surrogates for 99Tc (NaReO4) and 129I (NaI). Results showed that adding up to 10% of organoclay improved the retention of Re without adversely impacting hydraulic properties. To a lesser extent, iodine retention was also improved by adding up to 10% organoclay. Numerical modeling showed that using organoclay as a reactive barrier may significantly retard 99Tc release from saltstone disposal units.

  14. OLADE-Latin American and Caribbean Energy Efficiency Seminar...

    Open Energy Info (EERE)

    Panama, Mexico, Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Guyana, Paraguay, Peru, Suriname, Uruguay, Venezuela, Barbados, Cuba, Grenada, Haiti, Jamaica,...

  15. Energy-Economic Information System (SIEE) | Open Energy Information

    Open Energy Info (EERE)

    Panama, Mexico, Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Guyana, Paraguay, Peru, Suriname, Uruguay, Venezuela, Barbados, Cuba, Grenada, Haiti, Jamaica,...

  16. OLADE Sustainable Energy Planning Manual | Open Energy Information

    Open Energy Info (EERE)

    Panama, Mexico, Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Guyana, Paraguay, Peru, Suriname, Uruguay, Venezuela, Barbados, Cuba, Grenada, Haiti, Jamaica,...

  17. Legal Energy Information System (SIEL) Database | Open Energy...

    Open Energy Info (EERE)

    Panama, Mexico, Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Guyana, Paraguay, Peru, Suriname, Uruguay, Venezuela, Barbados, Cuba, Grenada, Haiti, Jamaica,...

  18. Caribbean-NREL Cooperation | Open Energy Information

    Open Energy Info (EERE)

    internatio Country Anguilla, Antigua and Barbuda, Aruba, Bahamas, Barbados, Cuba, Dominica, Dominican Republic, Grenada, Guadeloupe, Haiti, Jamaica, Martinique, Saint...

  19. Caribbean-GTZ Renewable Energy Program | Open Energy Information

    Open Energy Info (EERE)

    enpraxis95 Country Antigua & Barbuda, Aruba, Bahamas, Barbados, Cayman Islands, Cuba, Dominica, Dominican Republic, Grenada, Guadeloupe, Haiti, Jamaica, Martinique, Puerto...

  20. Humane Society International | Open Energy Information

    Open Energy Info (EERE)

    clinic for animals on the streets in Haiti, campaigns against factory farming in Mexico, improving farming practices in Brazil, and biodiversity conservation efforts in...

  1. NREL International Programs

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... NREL has assisted in bringing these basic services to clinics in Mexico, Ecuador, and Haiti, among others. For example, one Haitian hospital's surgery, dental clinic, lavatory, and ...

  2. NREL: Wind Research - International Wind Resource Maps

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cuba Dominican Republic El Salvador Fiji Islands Ghana Guatemala Haiti Honduras Indonesia (specific areas) Laos Mexico (specific areas) Mongolia Nicaragua Pakistan Papua New Guinea ...

  3. Energy Transition Initiative: Island Energy Snapshot - Dominican Republic

    SciTech Connect (OSTI)

    2015-09-01

    This profile provides a snapshot of the energy landscape of the Dominican Republic, a Caribbean nation that shares the island of Hispaniola with Haiti to the west.

  4. Category:Latin America Region | Open Energy Information

    Open Energy Info (EERE)

    Guatemala Guyana H Haiti Honduras J Jamaica M Martinique Mexico N Nicaragua P Panama Paraguay Peru S Saint Barthlemy Saint Kitts and Nevis Saint Lucia Saint Vincent and the...

  5. BPA-2014-00122-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    a campaign or expedition for which a campaign medal has been authorized, such as El Salvador, Lebanon, Granada, Panama, Southwest Asia, Somalia, and Haiti. You must submit a copy...

  6. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Haiti earthquake survivor to speak June 14, 2010 Los Alamos summer student describes mission to help rescuers LOS ALAMOS, New Mexico, June 14, 2010-When an earthquake struck Haiti last January, Christa Brelsford, a LANL student employee, was almost instantly trapped and partly crushed in the falling concrete of a building. She was saved by her brother and a friend, who hauled away debris for more than an hour before she was free. Now, with a prosthetic lower leg and a new view of life, the

  7. Western Wind and Solar Integration Study Phase 3A: Low Levels...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Renewable Energy Laboratory OEM original equipment manufacturer PV photovoltaic solar power, utility-scale photovoltaic power plant RAS remedial action scheme REMTF WECC ...

  8. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Shukurov, K.A.(a), A.M.Obukhov Institute of Atmospheric Physics, RAS (a), Russian Research Center "Kurchatov Institute" (b) Thirteenth Atmospheric Radiation Measurement...

  9. CRC handbook of agricultural energy potential of developing countries. Volume I

    SciTech Connect (OSTI)

    Duke, J.A.

    1986-01-01

    The contents of this book are: Introduction, Argentina, Bangladesh, Benin, Bolivia, Botswana, Bourkina (Upper Volta), Brazil, Burma, Burundi, Cameroon, Chad, Chile, Columbia, Costa Rica, Djibouti, Dominican Republic, Ecuador, El Salvador, Ethiopia, French Guiana, Gambia, Ghana, Guatemala, Guinea, Guyana, Haiti, Honduras, India, Indonesia, Jamaica, Appendix I. Conventional and Energetic Yields, Appendix II, Phytomass Files, and References.

  10. U.S. Energy Secretary Visits Qatar | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Qatar U.S. Energy Secretary Visits Qatar November 15, 2005 - 2:42pm Addthis Includes tour of Ras Laffan Industrial City and Port, opening of GE corporate office building, a visit to Education City, and time spent thanking U.S. troops DOHA, QATAR - U.S. Secretary of Energy Samuel W. Bodman today concluded a two-day visit to Qatar by participating in the announcement of Ras Laffan Liquefied Natural Gas Company III (RasGas III), visiting Qatar's Education City, and eating lunch with U.S. troops

  11. South America, Central America, the Caribbean, and Mexico

    SciTech Connect (OSTI)

    Deal, C.

    1981-10-01

    Summaries of oil and gas drillings, well completions, production, exploratory wells, exploration activity and wildcat drilling were given for South America, Central America, the Caribbean, and Mexico. The countries, islands, etc. included Argentina, Bahamas, Barbados, Belize, Bolivia, Brazil, Colombia, Costa Rica, Cuba, Dominican Republic, Ecuador, French Guiana, Guatemala, Guyana, Haiti, Honduras, Jamaica, Leeward and Windward Islands, Mexico, Netherlands Antilles, Nicaragua, Panama, Paraguay, Peru, Puerto Rico, El Salvador, Surinam, Trinidad and Venezuela. 16 figures, 120 tables. (DP)

  12. CyberShake3.0: Physics-Based Probabilistic Seismic Hazard Analysis |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Argonne Leadership Computing Facility CyberShake3.0: Physics-Based Probabilistic Seismic Hazard Analysis PI Name: Thomas Jordan PI Email: tjordan@usc.edu Institution: University of Southern California Allocation Program: INCITE Allocation Hours at ALCF: 2,000,000 Year: 2012 Research Domain: Earth Science Recent destructive earthquakes including Haiti (2010), Chile (2010), New Zealand( 2011), and Japan (2011) highlight the national and international need for improved seismic hazard

  13. CX-008164: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Captain Jack-Olinda RAS LLL/LT Replacement Project CX(s) Applied: B1.31 Date: 03/26/2012 Location(s): California Offices(s): Bonneville Power Administration

  14. Breaking a Pocket of Resistance in the Fight Against Cancer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaking a Pocket of Resistance in the Fight Against Cancer Breaking a Pocket of Resistance in the Fight Against Cancer Print Thursday, 12 December 2013 11:55 ras protein The new...

  15. Lower Yellowstone Intake Diversion Dam Fish Passage Project,...

    Energy Savers [EERE]

    ... U.S. Environmental Protection Agency ESA-Endangered Species Act fps-feet per second GIS-Geographic information system HEC-RAS-Hydrologic Engineering Center River Analysis ...

  16. Performance & Risk Assessment Community of Practice (P&RA CoP)

    Broader source: Energy.gov [DOE]

    Performance assessments (PAs) and risk assessments (RAs) evaluate the impact of a proposed remedial action on human health and the environment, and provide a demonstration of compliance and important technical inputs to meet regulatory requirements for: 1) waste form development and implementation; 2) tank closure activities; 3) waste site closure activities (e.g., cribs and trenches); 4) in-situ decontamination and decommissioning; 5) soil and groundwater remediation; and 6) management of disposal facilities (e.g., land-fills or near surface disposal facilities). The PAs and RAs or P&RAs become public documents upon completion. As such, the Department of Energy (DOE) needs to ensure that P&RAs continue to be performed and documented consistently and to high standards.

  17. '

    Office of Legacy Management (LM)

    ... Smith), 7evere 9ras9 ml Comer in Jetroit (P. :j. Todt) , Alum3 nwq Company of .imerica at ... CM. Fox, ':I. I4eAen) , Site Y (Cyril Smith), and Hat rolling work has been riono nt ...

  18. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Retrieval of Mean Cosine of Aerosol Phase Function from Extinction and Sky Brightness Measurements Zhuravleva, T.B.(a), Sviridenkov, M.A.(b), and Anikin, P.P.(b), Institute of Atmospheric Optics SB RAS, Tomsk, Russia (a), A.M. Obukhov Institute of Atmospheric Physics RAS, Moscow, Russia (b) Thirteenth Atmospheric Radiation Measurement (ARM) Science Team Meeting Asymmetry of the aerosol phase function together with optical thickness drive the magnitude of the aerosol radiative forcing. Two

  19. On Correction of Diffuse Radiation Measured by MFRSR

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    On Correction of Diffuse Radiation Measured by MFRSR T. B. Zhuravleva Institute of Atmospheric Optics, SB RAS Tomsk, Russia M. A. Sviridenkov and P. P. Anikin A. M. Obukhov Institute of Atmospheric Physics, RAS Moscow, Russia Introduction The multi-filter rotated shadowband radiometer (MFRSR) provides spectral direct, diffuse, and total horizontal solar irradiance measurements. Because the MFRSR's receiver has a non-Lambertian response, for a correct interpretation of measured radiation an

  20. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cloud Amount of Midlatitude Continental Clouds in Tomsk Region: Preliminary Results T.B. Zhuravleva, T.M. Rasskazchikova, and T.K. Sklyadneva Institute of Atmospheric Optics SB RAS Tomsk, Russia S.V. Smirnov Institute of Monitoring of Climatic and Ecological Systems SB RAS Tomsk, Russia The Atmospheric Radiation Measurement (ARM) Program provides concentrated measurements of surface radiative budget as well as measurements of the atmospheric constituents including clouds, aerosols, water vapor,

  1. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sub-Grid-Scale Isentropic Transports on McRAS Evaluations Using ARM-CART SCM Datasets Sud, Y.C., Walker, G.K., and Tao, W.-K., Climate and Radiation Branch, Laboratory for Atmospheres, NASA/Goddard Space Flight Center Fourteenth Atmospheric Radiation Measurement (ARM) Science Team Meeting Influence of Sub-grid-Scale Isentropic Transports on McRAS: Evaluation using ARM-CART SCM Datasets. Y. C. Sud, G. K. Walker and W.-K. Tao In GCM-physics evaluations with the currently available ARM-CART SCM

  2. Cutting-Balloon Angioplasty in Transplant Renal Artery Stenosis as First-Line Treatment in the Early Postoperative Period

    SciTech Connect (OSTI)

    Ucar, Adem; Yahyayev, Aghakishi; Bakkaloglu, Huseyin; Agayev, Ayaz; Aydin, Ali Emin; Rozanes, Izzet

    2011-02-15

    Percutaneous transluminal angioplasty has been successfully used for the treatment of transplant renal artery stenosis (RAS). Cutting-balloon angioplasty (CBA) is being used as a second option in pressure-resistant stenosis. It is thought that CBA is less traumatic and therefore restenosis occurs less frequently than in conventional angioplasty. This case report describes the unusual use of a cutting balloon in transplant RAS as a first option in the early postoperative period. Long-term follow-up data are also presented.

  3. Interagency Performance and Risk Assessment Community of Practice (P&RA

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CoP) Charter | Department of Energy Interagency Performance and Risk Assessment Community of Practice (P&RA CoP) Charter Interagency Performance and Risk Assessment Community of Practice (P&RA CoP) Charter Charter for the Interagency Performance and Risk Assessment Community of Practice (P&RA CoP) whose goal is to foster exchange of information regarding preparation of P&RAs across agencies and practitioners; enhance consistency in the preparation of P&RAs across the

  4. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Lenore Rasmussen, David Schramm, Lewis D. Meixler, Charles A. Gentile, George Ascione, Carl Tilson, and Kelsey Pagdon

    2010-02-19

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple, and now contract (new development) with low electric input. In addition, Ras Labs produces EAP materials that quickly contract and expand, repeatedly, by reversing the polarity of the electric input. These recent developments are important attributes in the field of electroactivity because of the ability of contraction and contraction-expansion to produce biomimetric motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to determine the mechanisms during contraction of these EAPs.

  5. RELIABILITY, AVAILABILITY, AND SERVICEABILITY FOR PETASCALE HIGH-END COMPUTING AND BEYOND

    SciTech Connect (OSTI)

    Chokchai "Box" Leangsuksun

    2011-05-31

    Our project is a multi-institutional research effort that adopts interplay of RELIABILITY, AVAILABILITY, and SERVICEABILITY (RAS) aspects for solving resilience issues in highend scientific computing in the next generation of supercomputers. results lie in the following tracks: Failure prediction in a large scale HPC; Investigate reliability issues and mitigation techniques including in GPGPU-based HPC system; HPC resilience runtime & tools.

  6. Bidirectional CLOCK/BMAL1-dependent circadian gene regulation by retinoic acid in vitro

    SciTech Connect (OSTI)

    Shirai, Hidenori; Oishi, Katsutaka; Ishida, Norio . E-mail: n.ishida@aist.go.jp

    2006-12-15

    A central circadian clock located in the suprachiasmatic nucleus (SCN) of the mammalian hypothalamus entrains peripheral clocks through both neural and humoral factors. Although candidates for entrainment factors have been described, their details remain obscure. Here, we screened ligands for nuclear receptors that affect CLOCK/BMAL1-dependent transactivation of the mouse Period1 (mPer1) gene in NIH3T3 cells. We found that retinoic acids (RAs) significantly up-regulate mPer1 expression in an E-box-dependent manner. We also found that RAs up-regulate the expression of other E-box-dependent circadian genes such as mPer2, arginine vasopressin (mAVP), and peroxisome proliferator-activated receptor {alpha} (mPPAR{alpha}). Surprisingly, the effect of RAs on CLOCK/BMAL1 (E-box)-dependent mRNA expression was bidirectional and depended on the presence of exogenous retinoic acid receptor {alpha} (RAR{alpha}). These results suggest that RAs regulate the CLOCK/BMAL1-dependent transcription of circadian genes in a complex manner.

  7. Eligibility for Renal Denervation: Anatomical Classification and Results in Essential Resistant Hypertension

    SciTech Connect (OSTI)

    Okada, Takuya Pellerin, Olivier; Savard, Sébastien; Curis, Emmanuel; Monge, Matthieu; Frank, Michael; Bobrie, Guillaume; Yamaguchi, Masato; Sugimoto, Koji; Plouin, Pierre-François; Azizi, Michel; Sapoval, Marc

    2015-02-15

    PurposeTo classify the renal artery (RA) anatomy based on specific requirements for endovascular renal artery denervation (RDN) in patients with drug-resistant hypertension (RH).Materials and MethodsThe RA anatomy of 122 consecutive RH patients was evaluated by computed tomography angiography and classified as two types: A (main RA ≥20 mm in length and ≥4.0 mm in diameter) or B (main RA <20 mm in length or main RA <4.0 mm in diameter). The A type included three subtypes: A1 (without accessory RAs), A2 (with accessory RAs <3.0 mm in diameter), and A3 (with accessory RAs ≥3.0 mm in diameter]. A1 and A2 types were eligible for RDN with the Simplicity Flex catheter. Type B included twi subtypes based on the main RA length and diameter. Patients were accordingly classified into three eligibility categories: complete (CE; both RAs were eligible), partial (PE; one eligible RA), and noneligibility (NE; no eligible RA).ResultsBilateral A1 type was the most prevalent and was observed in 48.4 % of the patients followed by the A1/A2 type (18 %). CE, PE, and NE were observed in 69.7, 22.9, and 7.4 % of patients, respectively. The prevalence of accessory RAs was 41 %.ConclusionsOf RH patients, 30.3 % were not eligible for bilateral RDN with the current Simplicity Flex catheter. This classification provides the basis for standardized reporting to allow for pooling of results of larger patient cohorts in the future.

  8. Acute ethanol intake induces superoxide anion generation and mitogen-activated protein kinase phosphorylation in rat aorta: A role for angiotensin type 1 receptor

    SciTech Connect (OSTI)

    Yogi, Alvaro; Callera, Glaucia E.; Mecawi, André S.; Batalhão, Marcelo E.; Carnio, Evelin C.; Antunes-Rodrigues, José; Queiroz, Regina H.; Touyz, Rhian M.; Tirapelli, Carlos R.

    2012-11-01

    Ethanol intake is associated with increase in blood pressure, through unknown mechanisms. We hypothesized that acute ethanol intake enhances vascular oxidative stress and induces vascular dysfunction through renin–angiotensin system (RAS) activation. Ethanol (1 g/kg; p.o. gavage) effects were assessed within 30 min in male Wistar rats. The transient decrease in blood pressure induced by ethanol was not affected by the previous administration of losartan (10 mg/kg; p.o. gavage), a selective AT{sub 1} receptor antagonist. Acute ethanol intake increased plasma renin activity (PRA), angiotensin converting enzyme (ACE) activity, plasma angiotensin I (ANG I) and angiotensin II (ANG II) levels. Ethanol induced systemic and vascular oxidative stress, evidenced by increased plasma thiobarbituric acid-reacting substances (TBARS) levels, NAD(P)H oxidase‐mediated vascular generation of superoxide anion and p47phox translocation (cytosol to membrane). These effects were prevented by losartan. Isolated aortas from ethanol-treated rats displayed increased p38MAPK and SAPK/JNK phosphorylation. Losartan inhibited ethanol-induced increase in the phosphorylation of these kinases. Ethanol intake decreased acetylcholine-induced relaxation and increased phenylephrine-induced contraction in endothelium-intact aortas. Ethanol significantly decreased plasma and aortic nitrate levels. These changes in vascular reactivity and in the end product of endogenous nitric oxide metabolism were not affected by losartan. Our study provides novel evidence that acute ethanol intake stimulates RAS activity and induces vascular oxidative stress and redox-signaling activation through AT{sub 1}-dependent mechanisms. These findings highlight the importance of RAS in acute ethanol-induced oxidative damage. -- Highlights: ► Acute ethanol intake stimulates RAS activity and vascular oxidative stress. ► RAS plays a role in acute ethanol-induced oxidative damage via AT{sub 1} receptor activation.

  9. Waveguide detection of right-angle-scattered light in flow cytometry

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P.

    2000-01-01

    A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.

  10. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Lenore Rasmussen, David Schramm, Paul Rasmussen, Kevin Mullaly, Ras Labs, LLC, Intelligent Materials for Prosthetics & Automation, Lewis D. Meixler, Daniel Pearlman and Alice Kirk

    2011-05-23

    Ras Labs produces contractile electroactive polymer (EAP) based materials and actuators that bend, swell, ripple, and contract (new development) with low electric input. In addition, Ras Labs produces EAP materials that quickly contract and expand, repeatedly, by reversing the polarity of the electric input, which can be cycled. This phenomenon was explored using molecular modeling, followed by experimentation. Applied voltage step functions were also investigated. High voltage steps followed by low voltage steps produced a larger contraction followed by a smaller contraction. Actuator control by simply adjusting the electric input is extremely useful for biomimetic applications. Muscles are able to partially contract. If muscles could only completely contract, nobody could hold an egg, for example, without breaking it. A combination of high and low voltage step functions could produce gross motor function and fine manipulation within the same actuator unit. Plasma treated electrodes with various geometries were investigated as a means of providing for more durable actuation.

  11. Structure of InP (001) surfaces prepared by decapping and by ion bombardment and annealing

    SciTech Connect (OSTI)

    Pahlke, D.; Kinsky, J.; Schultz, C.; Pristovsek, M.; Zorn, M.; Esser, N.; Richter, W.

    1997-07-01

    The structure of InP surfaces prepared by ion bombardment and annealing (IBA) and by decapping of InP (001) samples grown by metal organic vapor phase epitaxy is studied. The structural changes of the surfaces during preparation are monitored by low-energy electron diffraction and related to the surface electronic modifications as revealed by reflectance-anisotropy spectroscopy (RAS). For both preparation methods we find (2{times}4) reconstructions and almost identical RAS spectra. This finding contrasts with previous reports usually claiming a (4{times}2) surface after IBA Auger-electron spectroscopy as well as hydrogen-adsorbate vibrations recorded with high-resolution electron-energy spectroscopy indicate an In-rich surface stoichiometry. {copyright} {ital 1997} {ital The American Physical Society}

  12. Aerosol Radiative Forcing Under Cloudless Conditions.in Winter ZCAREX-2001

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Forcing Under Cloudless Conditions in Winter ZCAREX-2001 G. S. Golitsyn, I. A. Gorchakova, and I. I. Mokhov Institute of Atmospheric Physic Moscow, Russia Introduction Aerosol radiative forcing (ARF) is estimated for winter clear-sky conditions from measurements during ZCAREX-2001-Cloud-Aerosol-Radiation Experiment in February-March, 2001 at the Zvenigorod Scientific Station (ZSS) of the A.M. Obukhov Institute of Atmospheric Physics RAS. ARF in the shortwave range is determined by the difference

  13. Discovery of Next Generation RAF Inhibitors that Dissociate Paradoxical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Activation from Inhibition of the MAPK Pathway | Stanford Synchrotron Radiation Lightsource Discovery of Next Generation RAF Inhibitors that Dissociate Paradoxical Activation from Inhibition of the MAPK Pathway Monday, February 29, 2016 Genes encoding members of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway are frequently mutated in human cancer. RAS (a small GTPase) and RAF (a serine/Threonine kinase) are two major nodes on this important

  14. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Some Results of the Comparison of the Solar Almucantar Sky Brightness Observed Under the Cirri Conditions and the Calculated One Petrushin, A.G.(b), Shukurov, A.K.(a), Shukurov, K.A.(a), and Golitsyn, G.S.(a), A.M. Obukhov Institute of Atmospheric Physics, RAS (a), Institute of Experimental Meteorology, NPO "Typhoon" (b) Thirteenth Atmospheric Radiation Measurement (ARM) Science Team Meeting The selected measurements of the solar almucantar sky brightness were carried out at the

  15. Middle East: Output expansions boost drilling

    SciTech Connect (OSTI)

    1996-08-01

    Iraqi exports may return to the market in limited fashion, but none of the region`s producers seems particularly concerned. They believe that global oil demand is rising fast enough to justify their additions to productive capacity. The paper discusses exploration, drilling and development, and production in Saudi Arabia, Kuwait, the Neutral Zone, Abu Dhabi, Dubai, Oman, Iran, Iraq, Yemen, Qatar, Syria, Turkey, and Sharjah. The paper also briefly mentions activities in Bahrain, Israel, Jordan, and Ras al Khaimah.

  16. Somebody better find some rigs

    SciTech Connect (OSTI)

    1997-08-01

    The paper discusses the outlook for the gas and oil industries of the Middle East. Field development projects abound, as the larger exporting nations pursue ambitious policies of production expansion. However, their plans may be hampered by the growing worldwide shortage of rigs. Separate evaluations are given for Saudi Arabia, Kuwait, Neutral Zone, Abu Dhabi, Iran, Iraq, Qatar, Yemen, Syria, Dubai, Turkey, Sharjah, and briefly for Bahrain, Israel, Jordan, UAE-Ajman, and UAE-Ras al-Khaimah.

  17. Scientist's Quest For Artificial Muscle Aided by PPPL | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab Scientist's Quest For Artificial Muscle Aided by PPPL PPPL collaborator Lenore Rasmussen, the sole proprietor of her Princeton-based, startup firm, Ras Labs, LLC, has the gift of serendipity. Two disparate life experiences sparked the polymer chemist's interest in the development of electro-responsive "smart materials" - electrically-driven polymers that are strong and durable enough to act as artificial muscles in prosthetic devices and robotics. Publication File: PDF

  18. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Generalization of Stochastic Radiative Transfer Model: Multiple Broken Layers E. Kassianov Pacific Northwest National Laboratory Richland, Washington and Institute of Atmospheric Optics SB RAS Russia Introduction The problem of stochastic radiative transfer in clouds has been aimed at establishing the relationship between the statistical parameters of clouds and radiation. Although the necessity of such treatment was acknowledged long ago and several approaches were suggested (see, e.g., Cahalan

  19. Registration List - WCI-III

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    WCI-III Registration List Sandro Barlini, INFN - Laboratori Nazionali di Legnaro Wolfgang Bauer, Michigan State Aldo Bonasera, Laboratorio Nazionale del Sud, INFN Alexander Botvina, Institute for Nuclear Research RAS, Moscow Kyrill Bugaev, Lawrence Berkeley National Laboratory Xavier Campi, LPTMS Abdou Chbihi, GANIL Philippe Chomaz, GANIL Marco Cinausero, INFN Laboratori Nazionali di Legnaro Gianluca Colo', Dipartimento di Fisica Romualdo deSouza, Indiana University/IUCF Massimo Di Toro,

  20. kasyanov-98.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Effect of Three-Dimensional Cloud Inhomogeneity on Shortwave Radiative Transfer E. I. Kasyanov* and Y. L. Kogan CIMMS, University of Oklahoma Norman, Oklahoma *On leave from the Institute of Atmospheric Optics, RAS, Tomsk, Russia G. A. Titov Pacific Northwest National Laboratory Richland, Washington Introduction The effect of cloud inhomogeneity on radiative transfer in stratocumulus clouds, as well as retrieval of their optical properties, has been studied extensively during recent years.

  1. zuev(2)-99.PDF

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Impact of Volcanic Aerosol on Mean Fluxes of Solar Radiation in Broken Clouds V. V. Zuev and T. B. Zhuravleva Institute of Atmospheric Optics SB RAS Tomsk, Russia Introduction One of the powerful means of detecting the volcanic effect on the earth's radiation budget and, hence, on the earth's climate is numerical modeling. However, the atmospheric processes, during and after the eruption, are too complex and frequently prohibit an adequate description of the non-unique effect of volcanic

  2. zuev(3)-98.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Possible Forcing of the Greenhouse Phenomenon Under Stress of Plants and Soil Caused by Atmospheric Pollutants V. V. Zuev and Y. N. Ponomarev Institute of Atmospheric Optics SB RAS Tomsk, Russia Introduction Plant biosystems take an active part in planetary biochemical cycles and may affect chemical and physical characteristics of the earth's atmosphere. To estimate the long-term variation in concentration levels of CO 2 and other gases contributing to the greenhouse effect, the biospheric-

  3. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Spectral Fluxes of Solar Radiation in Broken Clouds: Algorithms for Calculation T. B. Zhuravleva and K. M. Firsov Institute of Atmospheric Optics SB RAS Tomsk, Russia Introduction The most Atmospheric Radiation Measurement (ARM) clear- and cloudy- sky radiation experi- ments have been performed with broadband fluxes. Because the fluxes are integrated over wavelength, it is difficult to understand the causes of unavoidable differences between calculated and observed broadband fluxes in those

  4. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regularities of Angular Distribution of Near-Horizon Sky Brightness in the Cloudless Atmosphere S.M. Sakerin, T.B. Zhuravleva, and I.M. Nasrtdinov Institute of Atomospheric Optics SB RAS Tomsk, Russia Introduction The methods of sun-photometry of the atmosphere based, for example, on interpretation of the angular distribution of radiation in the solar almucantar are widely used for retrieval of the aerosol optical characteristics (Dubovik et al. 2000). Preliminary analysis has shown that the

  5. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contribution to Visible Skylight Polarization as Measured at Different Values of Underlying Surface Albedo A.Kh. Shukurov, K.A. Shukurov, and GS Golitsyn A.M. Oboukhov Institute of Atmospheric Physics RAS Moscow, Russia Introduction Correlation coefficients R between the polarization P of scattered light radiating by the day sky as well as twilight sky and aerosol optical depth τ а , were analyzed in details at [1, 2] using the experimental data that had being measured till 1971. Most of the

  6. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Black Carbon Content, Aerosol Optical and Microphysical Characteristics in Moscow and the Moscow Region M.A. Sviridenkov, A.S. Emilenko, A.A. Isakov, and V.M. Kopeikin A.M. Obukhov Institute of Atmospheric Physics, RAS Moscow, Russia Introduction Aerosol loading in the atmosphere is determined by particle generation, growth, transport, and deposition processes. Large cities (e.g., Moscow) are significant sources of aerosols because soot acts as the main light-absorbing constituent. The urban

  7. Russian research capabilities: Findings of site visits

    SciTech Connect (OSTI)

    Wester, D.W.

    1994-02-01

    In June 1993, a proposal was presented to the International Environmental Institute (IEI) in Kennewick, Washington, to establish cooperation and coordination to further pursue the interests of the United States of America and the Republic of Russia in the application and promotion of environmental technology; characterization, treatment, handling, isolation, and disposal of hazardous and radioactive materials; conversion of defense sites to other purposes; and technology transfer, cooperative programs, joint technology development and contractual research. In response to this proposal, IEI and Pacific Northwest Laboratory (PNL) jointly provided funding to send Dr. Dennis W. Wester on a fact-finding mission to Novosibirsk, Moscow, and St. Petersburg, Russia. The trip covered a period of eight weeks, six of which were spent in Novosibirsk and adjoining or related cities and one of which was spent in each of Moscow and St. Petersburg. The general objectives of the trip were to establish a basis for cooperation between IEI and the Russian Academy of Sciences (RAS) for future coordination of mutual interests and objectives such as technology acquisition, development, demonstration, application, and commercialization; use of capabilities and assets developed by the U.S. Department of Energy (DOE) and the RAS; and expediting of cooperative agreements, personnel exchanges, joint ventures and other contractual relationships. The particular objectives of this trip were to evaluate the capabilities of the RAS to satisfy the technology needs associated with the cleanup of the Hanford Site and similar sites in the U.S. and to evaluate the expediency of establishing an IEI presence in Russia.

  8. Compositions and methods for detecting Noonan syndrome

    DOE Patents [OSTI]

    Gelb, Bruce D.; Tartaglia, Marco; Pennacchio, Len

    2012-07-17

    Diagnostic and therapeutic applications for Noonan Syndrome are described. The diagnostic and therapeutic applications are based on certain mutations in a RAS-specific guanine nucleotide exchange factor gene SOS1 or its expression product. The diagnostic and therapeutic applications are also based on certain mutations in a serine/threonine protein kinase gene RAF1 or its expression product thereof. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to RAF1 or SOS1, and variants thereof, as well as host cells expressing such variants.

  9. A high frequency resonance gravity gradiometer

    SciTech Connect (OSTI)

    Bagaev, S. N.; Kvashnin, N. L.; Skvortsov, M. N.; Bezrukov, L. B.; Krysanov, V. A.; Oreshkin, S. I.; Motylev, A. M.; Popov, S. M.; Samoilenko, A. A.; Yudin, I. S.; Rudenko, V. N.

    2014-06-15

    A new setup OGRANthe large scale opto-acoustical gravitational detector is described. As distinguished from known gravitational bar detectors it uses the optical interferometrical readout for registering weak variations of gravity gradient at the kilohetz frequency region. At room temperature, its sensitivity is limited only by the bar Brownian noise at the bandwidth close to 100 Hz. It is destined for a search for rare eventsgravitational pulses coincident with signals of neutrino scintillator (BUST) in the deep underground of Baksan Neutrino Observatory of INR RAS.

  10. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Estimation of Temperature Effect of Fires Near Moscow in Summer-Fall 2002 Mokhov, I.I. and Gorchakova, I.A., Obukhov Institute of Atmosphere Physics RAS, Russia Fourteenth Atmospheric Radiation Measurement (ARM) Science Team Meeting Local effect of cooling ΔT due to peatbog and forest fires near Moscow in summer-fall 2002 is estimated. These estimates are based on coordinated measurements at the Zvenigorod Scientific Station (55°42'N, 36°46'E) of our Institute. Continuous measurements of

  11. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    On Aerosol Optical Depth Determination from Zenith Measurements of Scattered Light Polarization Degree Shukurov, A.Kh., Shukurov K.A., and Golitsyn G.S., A.M. Obukhov Institute of Atmospheric Physics RAS Investigations on relationships between variations of aerosol optical depth τaer and maximal polarization degree Pmax of scattered light (from part of a sky within 90 angular distance of Sun in its almucantar or vertical) are well known (see e.g. Coulson K.L. Izvestia Atmospheric Oceanic

  12. Part III - List of Documents, Exhibits, and Other Attachments

    National Nuclear Security Administration (NNSA)

    Updated 9/30/15 to Mod 0588 Contract No. DE-AC04-94AL85000 Modification No. M202 Section J - Page 1 Part III - List of Documents, Exhibits, and Other Attachments Section J LIST OF ATTACHMENTS Document Appendix A - Personnel Appendix (Revised RAs 1-12 and Mods M0541, M0558) Appendix B - Statement of Work (Revised M244, M264) Appendix C - Special Bank Account Agreement (Revised M248, M271, M461, M497, M508) Appendix D - Key Personnel (Revised M216, M224, M227, M229, M233, M252, M257, M272, M275,

  13. Participant Obligations | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Updated 9/30/15 to Mod 0588 Contract No. DE-AC04-94AL85000 Modification No. M202 Section J - Page 1 Part III - List of Documents, Exhibits, and Other Attachments Section J LIST OF ATTACHMENTS Document Appendix A - Personnel Appendix (Revised RAs 1-12 and Mods M0541, M0558) Appendix B - Statement of Work (Revised M244, M264) Appendix C - Special Bank Account Agreement (Revised M248, M271, M461, M497, M508) Appendix D - Key Personnel (Revised M216, M224, M227, M229, M233, M252, M257, M272, M275,

  14. Crystal structure of inactive form of Rab3B

    SciTech Connect (OSTI)

    Zhang, Wei; Shen, Yang; Jiao, Ronghong; Liu, Yanli; Deng, Lingfu; Qi, Chao

    2012-06-28

    Rab proteins are the largest family of ras-related GTPases in eukaryotic cells. They act as directional molecular switches at membrane trafficking, including vesicle budding, cargo sorting, transport, tethering, and fusion. Here, we generated and crystallized the Rab3B:GDP complex. The structure of the complex was solved to 1.9 {angstrom} resolution and the structural base comparison with other Rab3 members provides a structural basis for the GDP/GTP switch in controlling the activity of small GTPase. The comparison of charge distribution among the members of Rab3 also indicates their different roles in vesicular trafficking.

  15. DOE-STD-3006-2000 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    00 DOE-STD-3006-2000 July 14, 2000 Planning and Conduct of Operational Readiness Reviews Replaced by DOE-STD-3006-2010 | Superseding DOE-STD-3006-95 (November 1995) DOE O 425.1B specifies the conditions and circumstances when an Operational Readiness Review (ORR) or a Readiness Assessment (RA) is required as part of a new start or restart process. This standard provides guidance on the planning and conduct of the ORRs and RAs. DOE-STD-3006-2000, Planning and Conduct of Operational Readiness

  16. DOE-STD-3006-95 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    95 DOE-STD-3006-95 December 05, 1995 Planning and Conduct of Operation Readiness Reviews Replaced by DOE-STD-3006-2000 DOE 0 425.1 specifies the conditions and circumstances when an Operational Readiness Review (ORR) or a Readiness Assessment (RA) is required as part of a new start or restart process. This standard provides guidance on the planning and conduct of the Operational Readiness Reviews. This standard also provides guidance for requesting exemptions. The requirements for ORRs and RAs

  17. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Comparison of the Data of Airborne, Lidar, and Photometric Sensing of Aerosol Parameters in the Cloudless Atmosphere M.V. Panchenko, V.S. Kozlov, Yu.S. Balin, S.M. Sakerin, G.P. Kokhanenko, L.E. Penner, D.M. Kabanov, E.V. Makienko, S.A. Terpugova, S.A. Turchinovich, and V.P. Shmargunov Institute of Atmospheric Optics SB RAS Tomsk, Russia Introduction A comprehensive investigation of the optical and microphysical parameters of tropospheric aerosol dictates the necessity of applying several

  18. Annotated bibliography of coal in the Caribbean region. [Lignite

    SciTech Connect (OSTI)

    Orndorff, R.C.

    1985-01-01

    The purpose of preparing this annotated bibliography was to compile information on coal localities for the Caribbean region used for preparation of a coal map of the region. Also, it serves as a brief reference list of publications for future coal studies in the Caribbean region. It is in no way an exhaustive study or complete listing of coal literature for the Caribbean. All the material was gathered from published literature with the exception of information from Cuba which was supplied from a study by Gordon Wood of the US Geological Survey, Branch of Coal Resources. Following the classification system of the US Geological Survey (Wood and others, 1983), the term coal resources has been used in this report for reference to general estimates of coal quantities even though authors of the material being annotated may have used the term coal reserves in a similar denotation. The literature ranges from 1857 to 1981. The countries listed include Colombia, Mexico, Venezuela, Cuba, the Dominican Republic, Haiti, Jamaica, Puerto Rico, and the countries of Central America.

  19. Proximal impact deposits at the Cretaceous-Tertiary boundary in the Gulf of Mexico: A restudy of DSDP Leg 77 Sites 536 and 540

    SciTech Connect (OSTI)

    Alvarez, W.; Asaro, F. ); Smit, J. ); Lowrie, W. ); Asaro, F. ); Margolis, S.V.; Claeys, P. ); Kastner, M. ); Hildebrand, A.R. )

    1992-08-01

    Restudy of Deep Sea Drilling Project Sites 536 and 540 in the southeast Gulf of Mexico gives evidence for a giant wave at Cretaceous-Tertiary boundary time. Five units are recognized: (1) Cenomanian limestone underlies a hiatus in which the five highest Cretaceous stages are missing, possibly because of catastrophic K-T erosion. (2) Pebbly mudstone, 45 m thick, represents a submarine landslide possibly of K-T age. (3) Current-bedded sandstone, more than 2.5 m thick, contains anomalous iridium, tektite glass, and shocked quartz; it is interpreted as ejecta from a nearby impact crater, reworked on the deep-sea floor by the resulting tsunami. (4) A 50-cm interval of calcareous mudstone containing small Cretaceous planktic foraminifera and the Ir peak is interpreted as the silt-size fraction of the Cretaceous material suspended by the impact-generated wave. (5) Calcareous mudstone with basal Tertiary forams and the uppermost tail of the Ir anomaly overlies the disturbed interval, dating the impact and wave event as K-T boundary age. Like Beloc in Haiti and Mimbral in Mexico, Sites 536 and 540 are consistent with a large K-T age impact at the nearby Chicxulub crater.

  20. Prospects for coal briquettes as a substitute fuel for wood and charcoal in US Agency for International Development Assisted countries

    SciTech Connect (OSTI)

    Perlack, R.D.; Stevenson, G.G.; Shelton, R.B.

    1986-02-01

    Fuelwood shortages and potential shortages are widespread throughout the developing world, and are becoming increasingly more prevalent because of the clearing of land for subsistence and plantation agriculture, excessive and inefficient commercial timber harvesting for domestic and export construction, and charcoal production to meet rising urban demands. Further, the environmental and socioeconomic consequences of the resulting deforestation are both pervasive and complex. This report focuses on the substitution of coal briquettes for fuelwood. Although substantial adverse health effects could be expected from burning non-anthracite coal or coal briquettes, a well-developed technique, carbonization, exists to convert coal to a safer form for combustion. The costs associated with briquetting and carbonizing coal indicate that ''smokeless'' coal briquettes can be produced at costs competitive with fuelwood and charcoal. The US Agency for International Development (USAID) is working on implementing this energy option in Haiti and Pakistan by (1) evaluating resources, (2) assessing markets, (3) analyzing technologies, (4) studying government policy and planning, and (5) packaging the idea for the private sector to implement. 26 refs., 2 figs., 12 tabs.

  1. Intervention in Countries with Unsustainable Energy Policies: Is it Ever Justifiable?

    SciTech Connect (OSTI)

    Tonn, Bruce Edward

    2010-08-01

    This paper explores whether it is ever justifiable for the international community to forcibly intervene in countries that have unsustainable energy policies. The literature on obligations to future generations suggests, philosophically, that intervention might be justified under certain circumstances. Additionally, the world community has intervened in the affairs of other countries for humanitarian reasons, such as in Kosovo, Somalia, and Haiti. However, intervention to deal with serious energy problems is a qualitatively different and more difficult problem. A simple risk analysis framework is used to organize the discussion about possible conditions for justifiable intervention. If the probability of deaths resulting from unsustainable energy policies is very large, if the energy problem can be attributed to a relatively small number of countries, and if the risk of intervention is acceptable (i.e., the number of deaths due to intervention is relatively small), then intervention may be justifiable. Without further analysis and successful solution of several vexing theoretical questions, it cannot be stated whether unsustainable energy policies being pursued by countries at the beginning of the 21st century meet the criteria for forcible intervention by the international community.

  2. Evolution of gas processing industry in Saudi Arabia

    SciTech Connect (OSTI)

    Showail, A.

    1983-01-01

    The beginning of the natural gas processing industry in Saudi Arabia is traced back to 1959 when Aramco embarked on a program to recover natural gas liquids (NGL) for export from low pressure gases such as stabilizer overhead, spheroid, tank farm, and refinery off-gases. The processing scheme involves compression and refrigeration to extract C3+ raw NGL, a raw NGL gathering system, and a fractionation plant to separate propane, butane, and natural gasoline. NGL extracted in Abqaiq and Ras Tanura is moved to Ras Tanura for fractionation, storage, and export. The system, built in several increments, has total design capacity of 500 MMscfd of feed gases to produce 320,000 bpd of NGL composed of 40% propane, 30% butane, and 30% natural gasoline. Phase II of the Saudi gas program envisages collection and processing of associated gas produced with Arabian medium and heavy crude oils largely in the northern onshore and offshore fields. Further domestic development may focus on more diversification in gas product utilization and on upgrading to higher value products.

  3. Geranylgeranylacetone inhibits ovarian cancer progression in vitro and in vivo

    SciTech Connect (OSTI)

    Hashimoto, Kae; Morishige, Ken-ichirou . E-mail: mken@gyne.med.osaka-u.ac.jp; Sawada, Kenjiro; Ogata, Seiji; Tahara, Masahiro; Shimizu, Shoko; Sakata, Masahiro; Tasaka, Keiichi; Kimura, Tadashi

    2007-04-27

    Geranylgeranylacetone (GGA), an isoprenoid compound, is an anti-ulcer drug developed in Japan. In our previous study, GGA was shown to inhibit ovarian cancer invasion by attenuating Rho activation [K. Hashimoto, K. Morishige, K. Sawada, M. Tahara, S. Shimizu, M. Sakata, K. Tasaka, Y. Murata, Geranylgeranylacetone inhibits lysophosphatidic acid-induced invasion of human ovarian carcinoma cells in vitro. Cancer 103 (2005) 1529-1536.]. In the present study, GGA treatment inhibited ovarian cancer progression in vitro and suppressed the tumor growth and ascites in the in vivo ovarian cancer model. In vitro analysis, treatment of cancer cells by GGA resulted in the inhibition of cancer cell proliferation, the inactivation of Ras, and the suppression of tyrosine phosphorylation of mitogen-activated protein kinase (MAPK). In conclusion, this is the first report that GGA inhibited ovarian cancer progression and the anti-tumor effect by GGA is, at least in part, derived not only from the suppression of Rho activation but also Ras-MAPK activation.

  4. The Adipose Renin-Angiotensin System Modulates Systemic Markers of Insulin Sensitivity and Activates the Intrarenal Renin-Angiotensin System

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kim, Suyeon; Soltani-Bejnood, Morvarid; Quignard-Boulange, Annie; Massiera, Florence; Teboul, Michele; Ailhaud, Gerard; Kim, Jung Han; Moustaid-Moussa, Naima; Voy, Brynn H.

    2006-01-01

    Background . The adipose tissue renin-angiotensin system (RAS) contributes to regulation of fat mass and may also impact systemic functions such as blood pressure and metabolism. Methods and results . A panel of mouse models including mice lacking angiotensinogen, Agt ( Agt -KO), mice expressing Agt solely in adipose tissue (aP2- Agt/Agt -KO), and mice overexpressing Agt in adipose tissue (aP2- Agt ) was studied. Total body weight, epididymal fat pad weight, and circulating levels of leptin, insulin, and resistin were significantly decreased in Agt -KO mice, while plasma adiponectin levels were increased. aP2- Agt mice exhibited increased adipositymore » and plasma leptin and insulin levels compared to wild type (WT) controls. Angiotensinogen and type I Ang II receptor protein levels were also elevated in kidney of aP2- Agt mice. Conclusion . These findings demonstrate that alterations in adipose RAS activity significantly impact both local and systemic physiology in a way that may contribute to the detrimental health effects of obesity.« less

  5. Remedial action plan and site design for stabilization of the inactive uranium processing site at Naturita, Colorado. Remedial Action Selection Report, Appendix B of Attachment 2: Geology report, Final

    SciTech Connect (OSTI)

    Not Available

    1994-03-01

    The uranium processing site near Naturita, Colorado, is one of 24 inactive uranium mill sites designated to be cleaned up by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA), 42 USC {section} 7901 et seq. Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which describes the proposed remedial action for the Naturita site. An extensive amount of data and supporting information has been generated and evaluated for this remedial action. These data and supporting information are not incorporated into this single document but are included or referenced in the supporting documents. The RAP consists of this RAS and four supporting documents or attachments. This Attachment 2, Geology Report describes the details of geologic, geomorphic, and seismic conditions at the Dry Flats disposal site.

  6. Soil Management Plan for the Oak Ridge Y-12 National Security Complex Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    2005-03-02

    This Soil Management Plan applies to all activities conducted under the auspices of the National Nuclear Security Administration (NNSA) Oak Ridge Y-12 National Security Complex (Y-12) that involve soil disturbance and potential management of waste soil. The plan was prepared under the direction of the Y-12 Environmental Compliance Department of the Environment, Safety, and Health Division. Soil disturbances related to maintenance activities, utility and building construction projects, or demolition projects fall within the purview of the plan. This Soil Management Plan represents an integrated, visually oriented, planning and information resource tool for decision making involving excavation or disturbance of soil at Y-12. This Soil Management Plan addresses three primary elements. (1) Regulatory and programmatic requirements for management of soil based on the location of a soil disturbance project and/or the regulatory classification of any contaminants that may be present (Chap. 2). Five general regulatory or programmatic classifications of soil are recognized to be potentially present at Y-12; soil may fall under one or more these classifications: (a) Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) pursuant to the Oak Ridge Reservation (ORR) Federal Facilities Agreement; (b) Resource Conservation and Recovery Act (RCRA); (c) RCRA 3004(u) solid waste managements units pursuant to the RCRA Hazardous and Solid Waste Amendments Act of 1984 permit for the ORR; (d) Toxic Substances and Control Act-regulated soil containing polychlorinated biphenyls; and (e) Radiologically contaminated soil regulated under the Atomic Energy Act review process. (2) Information for project planners on current and future planned remedial actions (RAs), as prescribed by CERCLA decision documents (including the scope of the actions and remedial goals), land use controls implemented to support or maintain RAs, RCRA post-closure regulatory requirements for

  7. Scanning the landscape of genome architecture of non-O1 and non-O139 Vibrio cholerae by whole genome mapping reveals extensive population genetic diversity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chapman, Carol; Henry, Matthew; Bishop-Lilly, Kimberly A.; Awosika, Joy; Briska, Adam; Ptashkin, Ryan N.; Wagner, Trevor; Rajanna, Chythanya; Tsang, Hsinyi; Johnson, Shannon L.; et al

    2015-03-20

    Historically, cholera outbreaks have been linked to V. cholerae O1 serogroup strains or its derivatives of the O37 and O139 serogroups. A genomic study on the 2010 Haiti cholera outbreak strains highlighted the putative role of non O1/non-O139 V. cholerae in causing cholera and the lack of genomic sequences of such strains from around the world. Here we address these gaps by scanning a global collection of V. cholerae strains as a first step towards understanding the population genetic diversity and epidemic potential of non O1/non-O139 strains. Whole Genome Mapping (Optical Mapping) based bar coding produces a high resolution, orderedmore » restriction map, depicting a complete view of the unique chromosomal architecture of an organism. To assess the genomic diversity of non-O1/non-O139 V. cholerae, we applied a Whole Genome Mapping strategy on a well-defined and geographically and temporally diverse strain collection, the Sakazaki serogroup type strains. Whole Genome Map data on 91 of the 206 serogroup type strains support the hypothesis that V. cholerae has an unprecedented genetic and genomic structural diversity. Interestingly, we discovered chromosomal fusions in two unusual strains that possess a single chromosome instead of the two chromosomes usually found in V. cholerae. We also found pervasive chromosomal rearrangements such as duplications and indels in many strains. The majority of Vibrio genome sequences currently in public databases are unfinished draft sequences. The Whole Genome Mapping approach presented here enables rapid screening of large strain collections to capture genomic complexities that would not have been otherwise revealed by unfinished draft genome sequencing and thus aids in assembling and finishing draft sequences of complex genomes. Furthermore, Whole Genome Mapping allows for prediction of novel V. cholerae non-O1/non-O139 strains that may have the potential to cause future cholera outbreaks.« less

  8. Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins

    SciTech Connect (OSTI)

    Deng, Lin; Lu, Yuanyuan; Zhao, Xiaodi; Sun, Yi; Shi, Yongquan; Fan, Hongwei; Liu, Changhao; Zhou, Jinfeng; Nie, Yongzhan; Wu, Kaichun; Fan, Daiming; Guo, Xuegang

    2013-10-18

    Highlights: •Overexpression of Ran in pancreatic cancer was correlated with histological grade. •Downregulation of Ran could induce cell apoptosis and inhibit cell proliferation. •The effects were mediated by cell cycle proteins, Survivin and cleaved Caspase-3. -- Abstract: Ran, a member of the Ras GTPase family, has important roles in nucleocytoplasmic transport. Herein, we detected Ran expression in pancreatic cancer and explored its potential role on tumour progression. Overexpressed Ran in pancreatic cancer tissues was found highly correlated with the histological grade. Downregulation of Ran led to significant suppression of cell proliferation, cell cycle arrest at the G1/S phase and induction of apoptosis. In vivo studies also validated that result. Further studies revealed that those effects were at least partly mediated by the downregulation of Cyclin A, Cyclin D1, Cyclin E, CDK2, CDK4, phospho-Rb and Survivin proteins and up regulation of cleaved Caspase-3.

  9. CANCELLED EMT and back again: does cellular plasticity fuelneoplasticprogressi on?

    SciTech Connect (OSTI)

    Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, MinaJ.

    2007-02-24

    Epithelial-mesenchymal transition (EMT) is a cellular transdifferentiation program that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. However, a similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, in which it is associated with disease progression. EMT in cancer epithelial cells often appears to be an incomplete and bi-directional process. Here we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the Ras-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  10. Mechanisms of disease: epithelial-mesenchymal transition and back again: does cellular plasticity fuel neoplastic progression?

    SciTech Connect (OSTI)

    Bissell, Mina J; Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, Mina J.

    2008-02-13

    Epithelial-mesenchymal transition (EMT) is a conversion that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. A similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, which is associated with disease progression. EMT in cancer epithelial cells often seems to be an incomplete and bi-directional process. In this Review, we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the RAS-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  11. Crystal structure of inactive form of Rab3B

    SciTech Connect (OSTI)

    Zhang, Wei; Shen, Yang; Jiao, Ronghong; Liu, Yanli; Deng, Lingfu; Qi, Chao

    2012-02-24

    Highlights: Black-Right-Pointing-Pointer This is the first structural information of human Rab3B. Black-Right-Pointing-Pointer To provides a structural basis for the GDP/GTP switch in controlling the activity of Rab3. Black-Right-Pointing-Pointer The charge distribution of Rab3B indicates its unique roles in vesicular trafficking. -- Abstract: Rab proteins are the largest family of ras-related GTPases in eukaryotic cells. They act as directional molecular switches at membrane trafficking, including vesicle budding, cargo sorting, transport, tethering, and fusion. Here, we generated and crystallized the Rab3B:GDP complex. The structure of the complex was solved to 1.9 A resolution and the structural base comparison with other Rab3 members provides a structural basis for the GDP/GTP switch in controlling the activity of small GTPase. The comparison of charge distribution among the members of Rab3 also indicates their different roles in vesicular trafficking.

  12. Nested methylation-specific polymerase chain reaction cancer detection method

    DOE Patents [OSTI]

    Belinsky, Steven A.; Palmisano, William A.

    2007-05-08

    A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.

  13. Fiscal Year 2007 Phased Construction Completion Report for the Zone 2 Soils, Slabs, and Subsurface Structures at East Tennessee Technology Park, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    RSI

    2008-03-01

    The purpose of this Phased Construction Completion Report (PCCR) is to present the fiscal year (FY) 2007 results of characterization activities and recommended remedial actions (RAs) for 11 exposure units (EUs) in Zone 2 (Z2-01, Z2-03, Z2-08, Z2-23, Z2-24, Z2-28, Z2-34, Z2-37, Z2-41, Z2-43, and Z2-44) at the East Tennessee Technology Park (ETTP), which is located in the northwest corner of the U.S. Department of Energy (DOE) Oak Ridge Reservation in Oak Ridge, Tennessee (Fig. 1). ETTP encompasses a total land area of approximately 5000 acres that has been subdivided into three zones--Zone 1 ({approx}1400 acres), Zone 2 ({approx}800 acres), and the Boundary Area ({approx}2800 acres). Zone 2, which encompasses the highly industrialized portion of ETTP shown in Fig. 1, consists of all formerly secured areas of the facility, including the large processing buildings and direct support facilities; experimental laboratories and chemical and materials handling facilities; materials storage and waste disposal facilities; secure document records libraries; and shipping and receiving warehouses. The Zone 2 Record of Decision for Soil, Buried Waste, and Subsurface Structure Actions in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE 2005) (Zone 2 ROD) specifies the future end use for Zone 2 acreage as uncontrolled industrial for the upper 10 ft of soils. Characterization activities in these areas were conducted in compliance with the Zone 2 ROD and the Dynamic Verification Strategy (DVS) and data quality objectives (DQOs) presented in the Remedial Design Report/Remedial Action Work Plan for Zone 2 Soils, Slabs, and Subsurface Structures, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE 2007) (Zone 2 RDR/RAWP). The purpose of this PCCR is to address the following: (1) Document DVS characterization results for the accessible EUs in FY 2007; (2) Describe and document the risk evaluation for each EU, and determine if the EU met the Zone 2 ROD requirements

  14. Evaluation of a biosolids minimization system

    SciTech Connect (OSTI)

    Bizier, P.A.

    1999-07-01

    The Micronair{trademark} residuals management system has been described by its manufacturer as a zero biosolids system. The system consists of three main parts--RAS screening, inerts removal, and an extremely fine bubble aeration system for the digester. The system's design assumes that trash and other non-biodegradable materials make up the bulk of residuals which would normally be digested. If these materials are removed, then the remaining biological material is assumed to biodegrade to either inerts or dissolved materials. This paper presents additional background on the design and operation of the residuals handling system. In addition, actual data from the facility detailing the operation of the residuals handling system. In addition, actual data from the facility detailing the operation of the Micronair{trademark} system since its initial start-up is provided. Finally, the benefits and drawbacks of the existing system are discussed and points for consideration in future installations identified.

  15. Report on the research conducted under the funding of the Sloan foundation postdoctoral fellowship in Computational Molecular Biology [Systematic study of protein-protein complexes] Final report

    SciTech Connect (OSTI)

    Sheinerman, Felix

    2001-06-01

    A central question in molecular biology is what structural features are common at protein-protein interfaces and what energetic factors define the affinity and specificity of protein-protein association. Analysis of structural and mutational data on protein-protein interfaces revealed that protein-protein interfaces of different functional classes contain many more energetically important charged and polar residues than was previously thought. Since, in the context of protein folding studies, polar interactions are believed to destabilize the folded proteins, this observation raised the question as to the forces that determine the stability of protein complexes. To investigate this issue in detail, the authors developed a number of partitioning schemes that allowed them to investigate the role of selected residues, ion pairs, and networks of polar interactions in protein-protein association. The methods developed were applied to the analysis of four different protein-protein interfaces: the ribonuclease barnase and its inhibitor barstar, the human growth hormone and its receptor, subtype N9 influenze virus neuraminidase and NC41 antibody, and the Ras Binding Domain of kinase cRaf and a Ras homologue Rap1A. The calculations revealed a surprising variability in how polar interactions affect the stability of different complexes. The finding that positions of charged and polar residues on protein-protein interfaces are optimized with respect to electrostatic interactions suggests that this property can be employed for the discrimination between native conformations and trial complexes generated by a docking algorithm. Analysis indicated the presence of SH2 domains in Janus family of non-receptor protein tyrosine kinases.

  16. 2010 Remediation Effectiveness Report for the U.S. Department of Energy Oak Ridge Reservation, Oak Ridge, Tennessee - Data and Evaluations

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2010-09-01

    Under the requirements of the Oak Ridge Reservation (ORR) Federal Facility Agreement (FFA) established between the U.S. Department of Energy (DOE), the U.S. Environmental Protection Agency, (EPA) and the Tennessee Department of Environment and Conservation (TDEC) in 1992, all environmental restoration activities on the ORR are performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Since the 1990s, the environmental restoration activities have experienced a gradual shift from characterization to remediation. As this has occurred, it has been determined that the assessment of the individual and cumulative performance of all ORR CERCLA remedial actions (RAs) is most effectively tracked in a single document. The Remediation Effectiveness Report (RER) is an FFA document intended to collate all ORR CERCLA decision requirements, compare pre- and post-remediation conditions at CERCLA sites, and present the results of any required post-decision remediation effectiveness monitoring. First issued in 1997, the RER has been reissued annually to update the performance histories of completed actions and to add descriptions of new CERCLA actions. Monitoring information used in the 2010 RER to assess remedy performance was collected and/or compiled by DOE's Water Resources Restoration Program (WRRP). Only data used to assess performance of completed actions are provided. In addition to collecting CERCLA performance assessment data, the WRRP also collects baseline data to be used to gauge the effectiveness of future actions once implemented. These baseline data are maintained in the Oak Ridge Environmental Information System and will be reported in future RERs, as necessary, once the respective actions are completed. However, when insufficient data exist to assess the impact of the RAs, e.g., when the RA was only recently completed, a preliminary evaluation is made of early indicators of effectiveness at the watershed

  17. Concanavalin A: A potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics

    SciTech Connect (OSTI)

    Li, Wen-wen; Yu, Jia-ying; Xu, Huai-long; Bao, Jin-ku

    2011-10-22

    Highlights: {yields} ConA induces cancer cell death targeting apoptosis and autophagy. {yields} ConA inhibits cancer cell angiogenesis. {yields} ConA is utilized in pre-clinical and clinical trials. -- Abstract: Concanavalin A (ConA), a Ca{sup 2+}/Mn{sup 2+}-dependent and mannose/glucose-binding legume lectin, has drawn a rising attention for its remarkable anti-proliferative and anti-tumor activities to a variety of cancer cells. ConA induces programmed cell death via mitochondria-mediated, P73-Foxo1a-Bim apoptosis and BNIP3-mediated mitochondrial autophagy. Through IKK-NF-{kappa}B-COX-2, SHP-2-MEK-1-ERK, and SHP-2-Ras-ERK anti-angiogenic pathways, ConA would inhibit cancer cell survival. In addition, ConA stimulates cell immunity and generates an immune memory, resisting to the same genotypic tumor. These biological findings shed light on new perspectives of ConA as a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis in pre-clinical or clinical trials for cancer therapeutics.

  18. Pizza.py Toolkit

    Energy Science and Technology Software Center (OSTI)

    2006-01-01

    Pizza.py is a loosely integrated collection of tools, many of which provide support for the LAMMPS molecular dynamics and ChemCell cell modeling packages. There are tools to create input files. convert between file formats, process log and dump files, create plots, and visualize and animate simulation snapshots. Software packages that are wrapped by Pizza.py. so they can invoked from within Python, include GnuPlot, MatLab, Raster3d. and RasMol. Pizza.py is written in Python and runs onmore » any platform that supports Python. Pizza.py enhances the standard Python interpreter in a few simple ways. Its tools are Python modules which can be invoked interactively, from scripts, or from GUIs when appropriate. Some of the tools require additional Python packages to be installed as part of the users Python. Others are wrappers on software packages (as listed above) which must be available on the users system. It is easy to modify or extend Pizza.py with new functionality or new tools, which need not have anything to do with LAMMPS or ChemCell.« less

  19. Protein receptor-independent plasma membrane remodeling by HAMLET: A tumoricidal protein-lipid complex

    SciTech Connect (OSTI)

    Nadeem, Aftab; Sanborn, Jeremy; Gettel, Douglas L.; James, Ho C. S.; Rydström, Anna; Ngassam, Viviane N.; Klausen, Thomas Kjaer; Pedersen, Stine Falsig; Lam, Matti; Parikh, Atul N.; Svanborg, Catharina

    2015-11-12

    A central tenet of signal transduction in eukaryotic cells is that extra-cellular ligands activate specific cell surface receptors, which orchestrate downstream responses. This ‘’protein-centric” view is increasingly challenged by evidence for the involvement of specialized membrane domains in signal transduction. Here, we propose that membrane perturbation may serve as an alternative mechanism to activate a conserved cell-death program in cancer cells. This view emerges from the extraordinary manner in which HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) kills a wide range of tumor cells in vitro and demonstrates therapeutic efficacy and selectivity in cancer models and clinical studies. We identify a ‘’receptor independent” transformation of vesicular motifs in model membranes, which is paralleled by gross remodeling of tumor cell membranes. Furthermore, we find that HAMLET accumulates within these de novo membrane conformations and define membrane blebs as cellular compartments for direct interactions of HAMLET with essential target proteins such as the Ras family of GTPases. In conclusion, we demonstrate lower sensitivity of healthy cell membranes to HAMLET challenge. These features suggest that HAMLET-induced curvature-dependent membrane conformations serve as surrogate receptors for initiating signal transduction cascades, ultimately leading to cell death.

  20. Protein receptor-independent plasma membrane remodeling by HAMLET: A tumoricidal protein-lipid complex

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nadeem, Aftab; Sanborn, Jeremy; Gettel, Douglas L.; James, Ho C. S.; Rydström, Anna; Ngassam, Viviane N.; Klausen, Thomas Kjaer; Pedersen, Stine Falsig; Lam, Matti; Parikh, Atul N.; et al

    2015-11-12

    A central tenet of signal transduction in eukaryotic cells is that extra-cellular ligands activate specific cell surface receptors, which orchestrate downstream responses. This ‘’protein-centric” view is increasingly challenged by evidence for the involvement of specialized membrane domains in signal transduction. Here, we propose that membrane perturbation may serve as an alternative mechanism to activate a conserved cell-death program in cancer cells. This view emerges from the extraordinary manner in which HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) kills a wide range of tumor cells in vitro and demonstrates therapeutic efficacy and selectivity in cancer models and clinical studies. Wemore » identify a ‘’receptor independent” transformation of vesicular motifs in model membranes, which is paralleled by gross remodeling of tumor cell membranes. Furthermore, we find that HAMLET accumulates within these de novo membrane conformations and define membrane blebs as cellular compartments for direct interactions of HAMLET with essential target proteins such as the Ras family of GTPases. In conclusion, we demonstrate lower sensitivity of healthy cell membranes to HAMLET challenge. These features suggest that HAMLET-induced curvature-dependent membrane conformations serve as surrogate receptors for initiating signal transduction cascades, ultimately leading to cell death.« less

  1. An association between the risk of ovarian cancer and rare HRAS1 alleles

    SciTech Connect (OSTI)

    Weitzel, J.N.; Patel, J.; Smith, D.M.

    1994-09-01

    The highly polymorphic HRAS1 minisatellite locus just downstream from the proto-oncogene H-ras-1 on chromosome 11p consists of four common progenitor alleles and several dozen rare alleles, which apparently derive from mutations of the progenitors. Mutant alleles of this locus represent a major risk factor for common types of cancer. Rare-sized HRAS1 alleles have been found more frequently in patients with carcinoma of the breast, colorectum, and urinary bladder and acute leukemia, compared to cancer-free controls. This highly significant association (p<1.001) results in a modest relative risk for patients with one rare allele, but the prevalence of this class of mutant alleles implies an important attributable risk: 1 in 11 cancers of the breast, colorectum, and bladder. Therefore, we performed a case-control study using conventional (Southern blot) and PCR-based methods to score HRAS1 alleles in the leukocyte DNA from 59 patients with ovarian cancer, and 51 cancer-free controls. Our preliminary data suggest an increased incidence of rare and intermediate HRAS1 alleles in caucasian ovarian cancer patients (13%) compared to cancer-free controls (4%). These results parallel the allele distribution seen in the large published series, and lend support for a significant association between rare HRAS1 alleles and ovarian cancer predisposition.

  2. PIK3CA is implicated as an oncogene in ovarian cancer

    SciTech Connect (OSTI)

    Shayesteh, Laleh; Lu, Yiling; Kuo, Wen-Lin; Baldocchi, Russell; Godfrey, Tony; Collins, Colin; Pinkel, Daniel; Powell, Bethan; Mills,Gordon B.; Gray, Joe W.

    1998-03-25

    Ovarian cancer is the leading cause of death from gynecological malignancy and the fourth leading cause of cancer death among American women, yet little is known about its molecular aetiology. Studies using comparative genomic hybridization (CGH) have revealed several regions of recurrent, abnormal, DNA sequence copy number that may encode genes involved in the genesis or progression of the disease. One region at 3q26 found to be increased in copy number in approximately 40 percent of ovarian and other cancers contains PIK3CA, which encodes the p110 a catalytic subunit of phosphatidylinositol 3-kinase(PI3-kinase). The association between PIK3CA copy number and PI3-kinase activity makes PIK3CA a candidate oncogene because a broad range of cancer-related functions have been associated with PI3-kinase mediated signaling. These include proliferation, glucose transport and catabolism, cell adhesion, apoptosis, RAS signaling and oncogenic transformation. In addition, downstream effectors of PI3-kinase,AKT1 and AKT2, have been found to be amplified or activated in human tumors, including ovarian cancer. We show here that PIK3CA is frequently increased in copy number in ovarian cancers, that the increased copy number is associated with increased PIK3CA transcription, p110 a protein expression and PI3-kinase activity and that treatment with the PI3-kinase inhibitor LY294002 decreases proliferation and increases apoptosis. Our observations suggest PIK3CA is an oncogene that has an important role in ovarian cancer.

  3. Remedial action plan and site design for stabilization of the inactive uranium processing site at Naturita, Colorado. Remedial action selection report, Attachment 2, Geology report: Preliminary final

    SciTech Connect (OSTI)

    Not Available

    1993-08-01

    The uranium processing site near Naturita, Colorado, is one of 24 inactive uranium mill sites designated to be cleaned up by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA), Public Law 95-604. Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which serves two purposes. First, it describes the activities that are proposed by the DOE to accomplish remediation and long-term stabilization and control of the radioactive materials at the inactive uranium processing site near Naturita, Colorado. Second, this document and the rest of the RAP, upon concurrence and execution by the DOE, the state of Colorado, and the NRC, become Appendix B of the cooperative agreement between the DOE and the State of Colorado.

  4. Guide to ground water remediation at CERCLA response action and RCRA corrective action sites

    SciTech Connect (OSTI)

    1995-10-01

    This Guide contains the regulatory and policy requirements governing remediation of ground water contaminated with hazardous waste [including radioactive mixed waste (RMW)], hazardous substances, or pollutants/contaminants that present (or may present) an imminent and substantial danger. It was prepared by the Office of Environmental Policy and Assistance, RCRA/CERCLA Division (EH-413), to assist Environmental Program Managers (ERPMs) who often encounter contaminated ground water during the performance of either response actions under CERCLA or corrective actions under Subtitle C of RCRA. The Guide begins with coverage of the regulatory and technical issues that are encountered by ERPM`s after a CERCLA Preliminary Assessment/Site Investigation (PA/SI) or the RCRA Facility Assessment (RFA) have been completed and releases into the environment have been confirmed. It is based on the assumption that ground water contamination is present at the site, operable unit, solid waste management unit, or facility. The Guide`s scope concludes with completion of the final RAs/corrective measures and a determination by the appropriate regulatory agencies that no further response action is necessary.

  5. Remedial action plan and site design for stabilization of the inactive uranium mill tailings site at Falls City, Texas. Remedial action selection report, attachment 2, geology report; attachment 3, groundwater hydrology report; and attachment 4, water resources protection strategy. Final report

    SciTech Connect (OSTI)

    1992-09-01

    The uranium processing site near Falls City, Texas, was one of 24 inactive uranium mill sites designated to be remediated by the U.S. Department of Energy (DOE) under Title I of the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). The UMTRCA requires that the U.S. Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the U.S. Environmental Protection Agency (EPA). The RAP, which includes this summary remedial action selection report (RAS), serves a two-fold purpose. First, it describes the activities proposed by the DOE to accomplish long-term stabilization and control of the residual radioactive materials at the inactive uranium processing site near Falls City, Texas. Second, this document and the remainder of the RAP, upon concurrence and execution by the DOE, the State of Texas, and the NRC, becomes Appendix B of the Cooperative Agreement between the DOE and the State of Texas.

  6. LNG fleet increases in size and capabilities

    SciTech Connect (OSTI)

    Linser, H.J. Jr.; Drudy, M.J.; Endrizzi, F.; Urbanelli, A.A.

    1997-06-02

    The LNG fleet as of early 1997 consisted of 99 vessels with total cargo capacity of 10.7 million cu m, equivalent to approximately 4.5 million tons. One of the newest additions to the fleet, the 137,000-cu m tanker Al Zubarah, is five times the size of the original commercial vessel Methane Princess. Al Zubarah`s first loading of more than 60,000 tons occurred in December 1996 for deliver to Japanese buyers from the newly commissioned Qatargas LNG plant at Ras Laffan. That size cargo contains enough clean-burning energy to heat 60,000 homes in Japan for 1 month. Measuring nearly 1,000 ft long, the tanker is among the largest in the industry fleet and joined 70 other vessels of more than 100,000 cu m. Most LNG tankers built since 1975 have been larger-capacity vessels. The paper discusses LNG shipping requirements, containment systems, vessel design, propulsion, construction, operations and maintenance, and the future for larger vessels.

  7. Development and application of procedures to evaluate air quality and visibility impacts of low-altitude flying operations

    SciTech Connect (OSTI)

    Liebsch, E.J.

    1990-08-01

    This report describes the development and application of procedures to evaluate the effects of low-altitude aircraft flights on air quality and visibility. The work summarized in this report was undertaken as part of the larger task of assessing the various potential environmental impacts associated with low-altitude military airspaces. Accomplishing the air quality/visibility analysis for the GEIS included (1) development and application of an integrated air quality model and aircraft emissions database specifically for Military Training Route (MTR) or similar flight operations, (2) selection and application of an existing air quality model to analyze the more widespread and less concentrated aircraft emissions from military Operations Areas (MOAs) and Restricted Areas (RAs), and (3) development and application of procedures to assess impacts of aircraft emissions on visibility. Existing air quality models were considered to be inadequate for predicting ground-level concentrations of pollutants emitted by aircraft along MTRs; therefore, the Single-Aircraft Instantaneous Line Source (SAILS) and Multiple-Aircraft Instantaneous Line Source (MAILS) models were developed to estimate potential impacts along MTRs. Furthermore, a protocol was developed and then applied in the field to determine the degree of visibility impairment caused by aircraft engine exhaust plumes. 19 refs., 2 figs., 3 tabs.

  8. Fiscal Year 2008 Phased Construction Completion Report for EU Z2-33 in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2008-09-11

    The Record of Decision for Soil, Buried Waste, and Subsurface Structure Actions in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2161&D2) (Zone 2 ROD) acknowledged that most of the 800 acres in Zone 2 were contaminated, but that sufficient data to confirm the levels of contamination were lacking. The Zone 2 ROD further specified that a sampling strategy for filling the data gaps would be developed. The Remedial Design Report/Remedial Action Work Plan for Zone 2 Soils, Slabs, and Subsurface Structures, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2224&D3) (Zone 2 RDR/RAWP) defined the sampling strategy as the Dynamic Verification Strategy (DVS), generally following the approach used for characterization of the Zone 1 exposure units (EUs). The Zone 2 ROD divided the Zone 2 area into seven geographic areas and 44 EUs. To facilitate the data quality objectives (DQOs) of the DVS process, the Zone 2 RDR/RAWP regrouped the 44 EUs into 12 DQO scoping EU groups. These groups facilitated the DQO process by placing similar facilities and their support facilities together and allowing identification of data gaps. The EU groups were no longer pertinent after DQO planning was completed and characterization was conducted as areas became accessible. As the opportunity to complete characterization became available, the planned DVS program and remedial actions (RAs) were completed for EU Z2-33. Remedial action was also performed at two additional areas in adjacent EU Z2-42 because of their close proximity and similar nature to a small surface soil RA in EU Z2-33. Remedial actions for building slabs performed in EU Z2-33 during fiscal year (FY) 2007 were reported in the Fiscal Year 2007 Phased Construction Completion Report for the Zone 2 Soils, Slabs, and Subsurface Structures at East Tennessee Technology Park, Oak Ridge, Tennessee (DOE/OR/01-2723&D1). Recommended RAs for EU Z2-42 were described in the Fiscal Year 2006 Phased Construction

  9. Considerations for Contractile Electroactive Polymeric Materials and Actuators

    SciTech Connect (OSTI)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Charles; Bernasek, Stephen L.; Abelev, Esta

    2009-06-16

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  10. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Carl; Bernasek, Stephen L.; Abelev, Esta

    2010-02-19

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  11. Mechanisms of radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.

    1996-10-01

    In the process of identifying genes differentially expressed in cells exposed ultraviolet radiation, we have identified a transcript having a 26-bp region that is highly conserved in a variety of species including Bacillus circulans, yeast, pumpkin, Drosophila, mouse, and man. When the 5` region (flanking region or UTR) of a gene, the sequence is predominantly in +/+ orientation with respect to the coding DNA strand; while in the coding region and the 3` region (UTR), the sequence is most frequently in the +/-orientation with respect to the coding DNA strand. In two genes, the element is split into two parts; however, in most cases, it is found only once but with a minimum of 11 consecutive nucleotides precisely depicting the original sequence. The element is found in a large number of different genes with diverse functions (from human ras p21 to B. circulans chitonase). Gel shift assays demonstrated the presence of a protein in HeLa cell extracts that binds to the sense and antisense single-stranded consensus oligomers, as well as to the double- stranded oligonucleotide. When double-stranded oligomer was used, the size shift demonstrated as additional protein-oligomer complex larger than the one bound to either sense or antisense single-stranded consensus oligomers alone. It is speculated either that this element binds to protein(s) important in maintaining DNA is a single-stranded orientation for transcription or, alternatively that this element is important in the transcription-coupled DNA repair process.

  12. 2011 Remediation Effectiveness Report for the U.S. Department of Energy Oak Ridge Reservation, Oak Ridge, Tennessee - Data and Evaluations

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2011-03-01

    Under the requirements of the Oak Ridge Reservation (ORR) Federal Facility Agreement (FFA) established between the U.S. Department of Energy (DOE), the U.S. Environmental Protection Agency, (EPA) and the Tennessee Department of Environment and Conservation (TDEC) in 1992, all environmental restoration activities on the ORR are performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Since the 1990s, the environmental restoration activities have experienced a gradual shift from characterization to remediation. As this has occurred, it has been determined that the assessment of the individual and cumulative performance of all ORR CERCLA remedial actions (RAs) is most effectively tracked in a single document. The Remediation Effectiveness Report (RER) is an FFA document intended to collate all ORR CERCLA decision requirements, compare pre- and post-remediation conditions at CERCLA sites, and present the results of any required post-decision remediation effectiveness monitoring. First issued in 1997, the RER has been reissued annually to update the performance histories of completed actions and to add descriptions of new CERCLA actions. Monitoring information used in the 2011 RER to assess remedy performance was collected and/or compiled by DOE's Water Resources Restoration Program (WRRP). Only data used to assess performance of completed actions are provided. In addition to collecting CERCLA performance assessment data, the WRRP also collects baseline data to be used to gauge the effectiveness of future actions once implemented. These baseline data are maintained in the Oak Ridge Environmental Information System and will be reported in future RERs, as necessary, once the respective actions are completed. However, when insufficient data exist to assess the impact of the RAs, e.g., when the RA was only recently completed, a preliminary evaluation is made of early indicators of effectiveness at the watershed

  13. Simvastatin induces apoptosis by a Rho-dependent mechanism in cultured cardiac fibroblasts and myofibroblasts

    SciTech Connect (OSTI)

    Copaja, Miguel; Venegas, Daniel; Aranguiz, Pablo; Canales, Jimena; Vivar, Raul; Catalan, Mabel; Olmedo, Ivonne; Rodriguez, Andrea E.; Chiong, Mario; Leyton, Lisette; Lavandero, Sergio; Diaz-Araya, Guillermo

    2011-08-15

    Several clinical trials have shown the beneficial effects of statins in the prevention of coronary heart disease. Additionally, statins promote apoptosis in vascular smooth muscle cells, in renal tubular epithelial cells and also in a variety of cell lines; yet, the effects of statins on cardiac fibroblast and myofibroblast, primarily responsible for cardiac tissue healing are almost unknown. Here, we investigated the effects of simvastatin on cardiac fibroblast and myofibroblast viability and studied the molecular cell death mechanism triggered by simvastatin in both cell types. Methods: Rat neonatal cardiac fibroblasts and myofibroblasts were treated with simvastatin (0.1-10 {mu}M) up to 72 h. Cell viability and apoptosis were evaluated by trypan blue exclusion method and by flow cytometry, respectively. Caspase-3 activation and Rho protein levels and activity were also determined by Western blot and pull-down assay, respectively. Results: Simvastatin induces caspase-dependent apoptosis of cardiac fibroblasts and myofibroblasts in a concentration- and time-dependent manner, with greater effects on fibroblasts than myofibroblasts. These effects were prevented by mevalonate, farnesylpyrophosphate and geranylgeranylpyrophosphate, but not squalene. These last results suggest that apoptosis was dependent on small GTPases of the Rho family rather than Ras. Conclusion: Simvastatin triggered apoptosis of cardiac fibroblasts and myofibroblasts by a mechanism independent of cholesterol synthesis, but dependent of isoprenilation of Rho protein. Additionally, cardiac fibroblasts were more susceptible to simvastatin-induced apoptosis than cardiac myofibroblasts. Thus simvastatin could avoid adverse cardiac remodeling leading to a less fibrotic repair of the damaged tissues. - Research Highlights: > Simvastatin decreases CF and CMF viability independent of cholesterol synthesis. > Simvastatin induces CF and CMF apoptosis in a caspase-dependent manner being CMF more resistant

  14. US DOE-EM On-Site Disposal Cell Working Group - Fostering Communication On Performance Assessment Challenges

    SciTech Connect (OSTI)

    Seitz, Roger R.; Suttora, Linda C.; Phifer, Mark

    2014-03-01

    On-site disposal cells are in use and being considered at several U.S. Department of Energy (USDOE) sites as the final disposition for large amounts of waste associated with cleanup of contaminated areas and facilities. These facilities are typically developed with regulatory oversight from States and/or the US Environmental Protection Agency (USEPA) in addition to USDOE. The facilities are developed to meet design standards for disposal of hazardous waste as well as the USDOE performance based standards for disposal of radioactive waste. The involvement of multiple and different regulators for facilities across separate sites has resulted in some differences in expectations for performance assessments and risk assessments (PA/RA) that are developed for the disposal facilities. The USDOE-EM Office of Site Restoration formed a working group to foster improved communication and sharing of information for personnel associated with these Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) disposal cells and work towards more consistent assumptions, as appropriate, for technical and policy considerations related to performance and risk assessments in support of a Record of Decision and Disposal Authorization Statement. The working group holds teleconferences, as needed, focusing on specific topics of interest. The topics addressed to date include an assessment of the assumptions used for performance assessments and risk assessments (PA/RAs) for on-site disposal cells, requirements and assumptions related to assessment of inadvertent intrusion, DOE Manual 435.1-1 requirements, and approaches for consideration of the long-term performance of liners and covers in the context of PAs. The working group has improved communication among the staff and oversight personnel responsible for onsite disposal cells and has provided a forum to identify and resolve common concerns.

  15. KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1

    SciTech Connect (OSTI)

    Goettel, Jeremy A.; Liang, Dongchun; Hilliard, Valda C.; Edelblum, Karen L.; Broadus, Matthew R.; Gould, Kathleen L.; Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232 ; Hanks, Steven K.; Polk, D. Brent

    2011-02-15

    The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway is a highly conserved signaling pathway that regulates diverse cellular processes including differentiation, proliferation, and survival. Kinase suppressor of Ras-1 (KSR1) binds each of the three ERK cascade components to facilitate pathway activation. Even though KSR1 contains a C-terminal kinase domain, evidence supporting the catalytic function of KSR1 remains controversial. In this study, we produced recombinant wild-type or kinase-inactive (D683A/D700A) KSR1 proteins in Escherichia coli to test the hypothesis that KSR1 is a functional protein kinase. Recombinant wild-type KSR1, but not recombinant kinase-inactive KSR1, underwent autophosphorylation on serine residue(s), phosphorylated myelin basic protein (MBP) as a generic substrate, and phosphorylated recombinant kinase-inactive MAPK/ERK kinase-1 (MEK1). Furthermore, FLAG immunoprecipitates from KSR1{sup -/-} colon epithelial cells stably expressing FLAG-tagged wild-type KSR1 (+KSR1), but not vector (+vector) or FLAG-tagged kinase-inactive KSR1 (+D683A/D700A), were able to phosphorylate kinase-inactive MEK1. Since TNF activates the ERK pathway in colon epithelial cells, we tested the biological effects of KSR1 in the survival response downstream of TNF. We found that +vector and +D683A/D700A cells underwent apoptosis when treated with TNF, whereas +KSR1 cells were resistant. However, +KSR1 cells were sensitized to TNF-induced cell loss in the absence of MEK kinase activity. These data provide clear evidence that KSR1 is a functional protein kinase, MEK1 is an in vitro substrate of KSR1, and the catalytic activities of both proteins are required for eliciting cell survival responses downstream of TNF.

  16. Azidothymidine and cisplatin increase p14ARF expression in OVCAR-3 ovarian cancer cell line

    SciTech Connect (OSTI)

    Vaskivuo, Liisa; Rysae, Jaana; Koivuperae, Johanna; Myllynen, Paeivi; Vaskivuo, Tommi; Chvalova, Katerina; Serpi, Raisa; Savolainen, Eeva-Riitta; Puistola, Ulla; Vaehaekangas, Kirsi . E-mail: kirsi.vahakangas@uku.fi

    2006-10-01

    p14{sup ARF} tumor suppressor protein regulates p53 by interfering with mdm2-p53 interaction. p14{sup ARF} is activated in response to oncogenic stimuli but little is known of the responses of endogenous p14{sup ARF} to different types of cellular stress or DNA damage. Azidothymidine (AZT) is being tested in several clinical trials as an enhancer of anticancer chemotherapy. However, the knowledge of the relationship between AZT and cellular pathways, e.g. p53 pathway, is very limited. In this study, we show that AZT, cisplatin (CDDP) and docetaxel (DTX) all induce unique molecular responses in OVCAR-3 ovarian carcinoma cells carrying a mutated p53, while in A2780, ovarian carcinoma and MCF-7 breast carcinoma cells with wild type p53, all of these drugs cause similar p53 responses. We found that endogenous p14{sup ARF} protein in OVCAR-3 cells is down-regulated by DTX but induced by AZT and a short CDDP pulse treatment. In HT-29 colon carcinoma cells with a mutated p53, all treatments down-regulated p14{sup ARF} protein. Both CDDP and AZT increased the expression of p14ARF mRNA in OVCAR-3 cells. Differences in cell death induced by these drugs did not explain the differences in protein and mRNA expressions. No increase in the level of either c-Myc or H-ras oncoproteins was seen in OVCAR-3 cells after AZT or CDDP-treatment. These results suggest that p14{sup ARF} can respond to DNA damage without oncogene activation in cell lines without functional p53.

  17. Expression analysis and prognostic significance of the SRA1 gene, in ovarian cancer

    SciTech Connect (OSTI)

    Leoutsakou, Theoni; Talieri, Maroulio; Scorilas, Andreas . E-mail: ascorilas@biol.uoa.gr

    2006-06-02

    The SR-related-CTD-associated-factors (SCAFs) have the ability to interact with the C-terminal domain of the RNA polymerase II, linking this way transcription to splicing. SRA1 (SR-A1) gene, encoding for a human high-molecular weight SCAF protein, is located on chromosome 19, between the IRF3 and the R-RAS oncogene and it has been demonstrated from members of our group that SRA1 is constitutively expressed in most of the human tissues, while it is overexpressed in a subset of ovarian tumors. In this study, we examine the expression of SRA1 gene in 111 ovarian malignant tissues and in the human ovarian carcinoma cell lines OVCAR-3, TOV21-G, and ES-2, using a semi-quantitative RT-PCR method. SRA1 gene was overexpressed in 61/111 (55%) of ovarian carcinomas. This higher expression was positively associated to the size of the tumor (p < 0.001), the grade and the stage of the disease (p = 0.003 and p = 0.006, respectively), and the debulking success (p < 0.001). Kaplan-Meier survival analysis revealed that lower SRA1 expression increases the probability of both the longer overall and the progression free survival of the patients. Multivariate Cox regression analysis revealed that SRA1 may be used as an independent prognostic biomarker in ovarian cancer. Our results suggest that SRA1 is associated with cancer progression and may possibly be characterized as a new marker of unfavorable prognosis for ovarian cancer.

  18. Transplacental arsenic plus postnatal 12-O-teradecanoyl phorbol-13-acetate exposures associated with hepatocarcinogenesis induce similar aberrant gene expression patterns in male and female mouse liver

    SciTech Connect (OSTI)

    Liu Jie . E-mail: Liu6@niehs.nih.gov; Xie Yaxiong; Merrick, B. Alex; Shen Jun; Ducharme, Danica M.K.; Collins, Jennifer; Diwan, Bhalchandra A.; Logsdon, Daniel; Waalkes, Michael P.

    2006-06-15

    Our prior work shows that in utero arsenic exposure alone is a complete transplacental carcinogen, producing hepatocellular carcinoma in adult male offspring but not in females. In a follow-up study to potentially promote arsenic-initiated tumors, mice were exposed to arsenic (85 ppm) from gestation day 8 to 18 and then exposed to 12-O-teradecanoyl phorbol-13-acetate (TPA), a well-known tumor promoter after weaning. The dermal application of TPA (2 {mu}g/0.1 ml acetone, twice/week for 21 weeks) after transplacental arsenic did not further increase arsenic-induced liver tumor formation in adult males but significantly increased liver tumor formation in adult females. Thus, for comparison, liver tumors and normal liver samples taken from adult male and female mice at necropsy were analyzed for aberrant gene/protein expression by microarray, real-time RT-PCR and Western blot analysis. Arsenic/TPA treatment resulted in increased expression of {alpha}-fetoprotein, k-ras, c-myc, estrogen receptor-{alpha}, cyclin D1, cdk2na, plasminogen activator inhibitor-1, cytokeratin-8, cytokeratin-18, glutathione S-transferases and insulin-like growth factor binding proteins in liver and liver tumors from both male and female mice. Arsenic/TPA also decreased the expression of BRCA1, betaine-homocysteine methyltransferase, CYP7B1, CYP2F2 and insulin-like growth factor-1 in normal and cancerous livers. Alterations in these gene products were associated with arsenic/TPA-induced liver tumors, regardless of sex. Thus, transplacental arsenic plus postnatal TPA exposure induced similar aberrant gene expression patterns in male and female mouse liver, which are persistent and potentially important to the mechanism of arsenic initiation of hepatocarcinogenesis.

  19. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion

    SciTech Connect (OSTI)

    Cekanova, Maria; Fernando, Romaine I.; Siriwardhana, Nalin; Sukhthankar, Mugdha; Parra, Columba de la; Woraratphoka, Jirayus; Malone, Christine; Ström, Anders; Baek, Seung J.; Wade, Paul A.; Saxton, Arnold M.; Donnell, Robert M.; Pestell, Richard G.; and others

    2015-02-01

    We have previously demonstrated that the anti-apoptotic protein BAD is expressed in normal human breast tissue and shown that BAD inhibits expression of cyclin D1 to delay cell-cycle progression in breast cancer cells. Herein, expression of proteins in breast tissues was studied by immunohistochemistry and results were analyzed statistically to obtain semi-quantitative data. Biochemical and functional changes in BAD-overexpressing MCF7 breast cancer cells were evaluated using PCR, reporter assays, western blotting, ELISA and extracellular matrix invasion assays. Compared to normal tissues, Grade II breast cancers expressed low total/phosphorylated forms of BAD in both cytoplasmic and nuclear compartments. BAD overexpression decreased the expression of β-catenin, Sp1, and phosphorylation of STATs. BAD inhibited Ras/MEK/ERK and JNK signaling pathways, without affecting the p38 signaling pathway. Expression of the metastasis-related proteins, MMP10, VEGF, SNAIL, CXCR4, E-cadherin and TlMP2 was regulated by BAD with concomitant inhibition of extracellular matrix invasion. Inhibition of BAD by siRNA increased invasion and Akt/p-Akt levels. Clinical data and the results herein suggest that in addition to the effect on apoptosis, BAD conveys anti-metastatic effects and is a valuable prognostic marker in breast cancer. - Highlights: • BAD and p-BAD expressions are decreased in breast cancer compared with normal breast tissue. • BAD impedes breast cancer invasion and migration. • BAD inhibits the EMT and transcription factors that promote cancer cell migration. • Invasion and migration functions of BAD are distinct from the BAD's role in apoptosis.

  20. Twenty years of sedimentary change and diagenesis, Qatar Peninsula, Arabian Gulf

    SciTech Connect (OSTI)

    Shinn, E.A.

    1988-02-01

    Re-examination in February 1986 of areas studied by the author in 1966 and 1967 revealed rapid rates of sedimentation and diagenesis along the east coast of the Qatar Peninsula. At Ras Um Sa on the north-east side of Qatar, a series of chenier-like beaches and curved spits is building southward under the influence of longshore currents. Beach and spit growth increasingly protects the shoreline and allows tidal flats to form in their lee. As these spits accrete, they become armored on their lagoonal side by beachrock formation. In the last 20 years a spit approximately 1/2 km long has grown, and beachrock has already armored its recurved lagoonal side. At Umm Said on the southeast side of Qatar, seawardly accreting barchan dunes composed of quartz sand have built a 40-km long, 10-km wide sabkha as thick as 30 m. Aerial and ground photographs show that certain dunes near the seaward edge of the sabkha have migrated into the sea and have contributed to shoreline accretion. The observed rate of dune migration suggests that within approximately 100 years the remaining quartz sands will be depleted and blown into the sea because there is no replenishment of the dune field. Subsequently, the area will revert to one of carbonate deposition. Fine-grained dolomite is presently precipitating in interstitial brines in the landwardmost portions of this unusual sabkha. In the geologic record, such a deposit would be an anomalous, porous and permeable, cross-bedded, linear, 30-m thick, dolomite-cemented sandstone body encased in carbonate sediments. A modern model such as the one at Umm Said can provide useful clues for determining the origin of similar deposits in ancient rocks.

  1. Identification of a genetic interaction between the tumor suppressor EAF2 and the retinoblastoma protein (Rb) signaling pathway in C. elegans and prostate cancer cells

    SciTech Connect (OSTI)

    Cai, Liquan; Wang, Dan; Fisher, Alfred L.; Wang, Zhou

    2014-05-02

    Highlights: RNAi screen identified genetic enhancers for the C. elegans homolog of EAF2. EAF2 and RBBP4 proteins physically bind to each other and alter transcription. Overexpression of EAF2 and RBBP4 induces the cell death in prostate cancer cells. - Abstract: The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. While EAF2 and its partner ELL have been shown to be members of protein complexes involved in RNA polymerase II transcriptional elongation, the biologic roles for EAF2 especially with regards to the development of cancer remains poorly understood. We have previously identified the eaf-1 gene in Caenorhabditiselegans as the ortholog of EAF2, and shown that eaf-1 interacts with the ELL ortholog ell-1 to control development and fertility in worms. To identify genetic pathways that interact with eaf-1, we screened RNAi libraries consisting of transcription factors, phosphatases, and chromatin-modifying factors to identify genes which enhance the effects of eaf-1(tm3976) on fertility. From this screen, we identified lin-53, hmg-1.2, pha-4, ruvb-2 and set-6 as hits. LIN-53 is the C. elegans ortholog of human retinoblastoma binding protein 4/7 (RBBP 4/7), which binds to the retinoblastoma protein and inhibits the Ras signaling pathway. We find that lin-53 showed a synthetic interaction with eaf-1(tm3976) where knockdown of lin-53 in an eaf-1(tm3976) mutant resulted in sterile worms. This phenotype may be due to cell death as the treated worms contain degenerated embryos with increased expression of the ced-1:GFP cell death marker. Further we find that the interaction between eaf-1 and lin-53/RBBP4/7 also exists in vertebrates, which is reflected by the formation of a protein complex between EAF2 and RBBP4/7. Finally, overexpression of either human EAF2 or RBBP4 in LNCaP cells induced the cell death while knockdown of EAF2 in LNCaP enhanced cell proliferation, indicating an important role of EAF2 in controlling

  2. The RERTR Program : a status report.

    SciTech Connect (OSTI)

    Travelli, A.

    1998-10-19

    major milestone was accomplished in the development of a process to produce molybdenum-99 from fission targets utilizing LEU instead of HEU. Targets containing LEU metal foils were irradiated in the RAS-GAS reactor at BATAN, Indonesia, and molybdenum-99 was successfully extracted through the ensuing process. These are exciting times for the program and for all those involved in it, and last year's successes augur well for the future. However, as in the past, the success of the RERTR program will depend on the international friendship and cooperation that have always been its trademark.

  3. Aquatic Natural Areas Analysis and Evaluation: Oak Ridge Reservation

    SciTech Connect (OSTI)

    Baranski, Dr. Michael J.

    2011-04-01

    maximum score possible. A highly ranked ANA or ARA is one that is large in size compared to other areas, includes a greater proportion of the watershed within Reservation boundaries, contains a number of status taxa at high densities, exhibits a high overall biodiversity, has very good or excellent habitat and water quality, is well protected and isolated from disturbances, and shows several other characteristics that contribute to natural area value. In this report, the term 'natural area' is loosely defined as a terrestrial or aquatic system that exhibits, or is thought to exhibit, high natural integrity and other significant natural values. The purpose of the present study is to evaluate and rank the currently recognized Aquatic Natural Areas (ANAs) and Aquatic Reference Areas (ARAs) on the Oak Ridge Reservation (ORR) for their natural area value. A previous study (Baranski 2009) analyzed, evaluated, and ranked terrestrial areas (Natural Areas [NAs], Reference Areas [RAs], and Cooperative Management Areas [CMAs]) on the ORR for natural area value, and a precise methodology for natural area evaluation was developed. The present study is intended to be a complement and companion to the terrestrial area study and attempts to employ a similar methodology for aquatic areas so that aquatic and terrestrial areas can be compared on a similar scale. This study specifically develops criteria for assessing the ecological, biodiversity, and natural area importance and significance of aquatic systems on the Reservation in a relevant and consistent manner. The information can be integrated into the Tennessee Natural Heritage Program (http://tn.gov/environment/na/nhp.shtml) system and applied to potential new aquatic areas. Further, the information will be useful in planning, management, and protection efforts on the ORR.

  4. Assssment and Mapping of the Riverine Hydrokinetic Resource in the Continental United States

    SciTech Connect (OSTI)

    Jacobson, Paul T.; Ravens, Thomas M.; Cunningham, Keith W.; Scott, George

    2012-12-14

    Laboratory?s Virtual Hydropower Prospector, Google Earth, and U.S. Geological Survey gages. Data were manually obtained for the eleven largest rivers with average flow rates greater than 10,000 cfs and the resulting estimate of the theoretical resource was expanded to include rivers with discharge between 1,000 cfs and 10,000 cfs based upon the contribution of rivers in the latter flow class to the total estimate in the contiguous 48 states. Segment-specific theoretical resource was aggregated by major hydrologic region in the contiguous, lower 48 states and totaled 1,146 TWh/yr. The aggregate estimate of the Alaska theoretical resource is 235 TWh/yr, yielding a total theoretical resource estimate of 1,381 TWh/yr for the continental US. The technically recoverable resource in the contiguous 48 states was estimated by applying a recovery factor to the segment-specific theoretical resource estimates. The recovery factor scales the theoretical resource for a given segment to take into account assumptions such as minimum required water velocity and depth during low flow conditions, maximum device packing density, device efficiency, and flow statistics (e.g., the 5 percentile flow relative to the average flow rate). The recovery factor also takes account of ?back effects? ? feedback effects of turbine presence on hydraulic head and velocity. The recovery factor was determined over a range of flow rates and slopes using the hydraulic model, HEC-RAS. In the hydraulic modeling, presence of turbines was accounted for by adjusting the Manning coefficient. This analysis, which included 32 scenarios, led to an empirical function relating recovery factor to slope and discharge. Sixty-nine percent of NHDPlus segments included in the theoretical resource estimate for the contiguous 48 states had an estimated recovery factor of zero. For Alaska, data on river slope was not readily available; hence, the recovery factor was estimated based on the flow rate alone. Segment-specific estimates of