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  1. Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap...

    Open Energy Info (EERE)

    Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap and Strategy Jump to: navigation, search Name Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap and...

  2. Nutritional Analysis of Thomazeau, Haiti 

    E-Print Network [OSTI]

    Vanderpool, John Mark

    2013-02-04

    ????????????????????????..9 III. RESULTS??????????????????????..??11 IV. CONCLUSIONS????????????????????..?.13 REFERENCES??????????????????????????.??.14 ! ! 2! ! ! ABSTRACT Nutritional Analysis of Thomazeau, Haiti. (May 2013) John Mark... stream_source_info VANDERPOOL-THESIS-2013.pdf.txt stream_content_type text/plain stream_size 14539 Content-Encoding ISO-8859-1 stream_name VANDERPOOL-THESIS-2013.pdf.txt Content-Type text/plain; charset=ISO-8859...

  3. Economic feasibility of bagasse charcoal in Haiti

    E-Print Network [OSTI]

    Kamimoto, Lynn K. (Lynn Kam Oi)

    2005-01-01

    The economics of implementing bagasse-based charcoal manufacturing in Haiti was investigated. From these main inputs, three different manufacturing economic scenarios were modeled using a simple, dynamic excel spreadsheet. ...

  4. Converting sugarcane waste into charcoal for Haiti

    E-Print Network [OSTI]

    Toussaint, Etienne Clement

    2007-01-01

    In Haiti, most families have traditionally relied on wood and wood-derived charcoal as their primary fuel source for indoor cooking. This resource has proven to be unsustainable, however, as over 90% of the Haitian countryside ...

  5. Digital Signaling and Hysteresis Characterize Ras Activation in Lymphoid Cells

    E-Print Network [OSTI]

    Das, Jayajit

    Activation of Ras proteins underlies functional decisions in diverse cell types. Two molecules, RasGRP and SOS, catalyze Ras activation in lymphocytes. Binding of active Ras to SOS' allosteric pocket markedly increases ...

  6. Selected critical bibliography of English-language books on Haiti

    E-Print Network [OSTI]

    Freeman, Bryant C.

    1995-01-01

    , from French) London: Zed, 1985. Pp. 308. Shocking eye­ witness account by a French journalist of life of Haitian sugarcane cutters in the Dominican Republic, amounting to a form of modern-day slavery. Leon, Warren E., Jr. Peintures haitiennes... consultant of economic and political conditions in the Dominican Republic and Haiti, explaining the virtual slavery of the Haitian sugarcane cutters in the D.R. Followed by eye-witness account of actual conditions. 17 Plummer, Brenda Gayle. Haiti...

  7. La situation politique et sociale en Haiti : aout 1963

    E-Print Network [OSTI]

    International Commission of Jurists (1952- )

    2000-01-01

    live: suffice it for us to recall a few facts and statistics widely disseminated by the media in recent weeks. The Republic of Haiti has received since 1954 from the United States financial aid estimated to be 53 million dollars and loans from... live: suffice it for us to recall a few facts and statistics widely disseminated by the media in recent weeks. The Republic of Haiti has received since 1954 from the United States financial aid estimated to be 53 million dollars and loans from...

  8. The Faces of Haiti: Resolute in Reform, Resistance and Recovery

    E-Print Network [OSTI]

    Graham, Maryemma; Rosenblum, Brian; Jayaram, Kiran C.; Claiborne, C. B.

    2011-10-25

    . It was not the earthquake alone that generated our inter- est in a visit to Haiti. KU has been home for the Institute for Haitian Studies since 1992, when it was founded by Bryant Freeman, currently an emeritus professor of French and Italian. While some of our team were... This was their fifth invitation- al workshop and the second held since the earthquake. As interest in Haiti has increased, INURED has stepped up its effort to play a think-tank role. We came to the meet- ing without an agenda, which served to our advantage. We...

  9. RAs

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal GasAdministration Medal01 Sandia4) August 20123/%2A en NNSA sitesInformation/%2A en9/%2A

  10. Selected critical bibliography of English-language books on Haiti

    E-Print Network [OSTI]

    Freeman, Bryant C.

    1998-01-01

    of Haitian sugarcane cutters in the Dominican Republic, amounting to a form of modern-day slavery. Leon, Warren E., Jr. Peintures haïtiennes. Boulogne, France: Delroisse, 1978. Pp. 143. Text in French, English, Spanish, and German. Explanatory notes quite... Jersey: Zed Books, 1987. Pp. xiv-177. Excellent summary by a British human rights consultant of economic and political conditions in the Dominican Republic and Haiti, explaining the virtual slavery of the Haitian sugarcane cutters in the D.R. Followed...

  11. Energy Transition Initiative: Island Energy Snapshot - Haiti; U.S. Department of Energy (DOE), NREL (National Renewable Energy Laboratory)

    SciTech Connect (OSTI)

    2015-06-01

    This profile provides a snapshot of the energy landscape of Haiti, an independent nation that occupies the western portion of the island of Hispaniola in the northern Caribbean Sea. Haiti’s utility rates are roughly $0.35 U.S. dollars (USD) per kilowatt-hour (kWh), above the Caribbean regional average of $0.33 USD/kWh.

  12. Earthquake Damage Assessment Using Objective Image Segmentation: A Case Study of 2010 Haiti Earthquake

    E-Print Network [OSTI]

    Earthquake Damage Assessment Using Objective Image Segmentation: A Case Study of 2010 Haiti Earthquake Thomas Oommen1 , Umaa Rebbapragada2 , and Daniel Cerminaro3 1 Department of Geological Engineering Haiti earthquake provided a new opportunity to analyze earthquake-induced damage. Previous work has

  13. Design and implementation of a solar power system in rural Haiti

    E-Print Network [OSTI]

    Hussam, Shaheer M. (Shaheer Muqtasid), 1981-

    2004-01-01

    This paper describes the design and implementation of a solar power system for a school and health center in Petit-Anse, Haiti. The end-use applications are lighting via a set of fluorescent and incandescent bulbs, and a ...

  14. Design of a bagasse charcoal briquette-making device for use in Haiti

    E-Print Network [OSTI]

    Vechakul, Jessica

    2005-01-01

    Charcoal made from bagasse, the fibrous remains of sugarcane production, has the potential to serve as an alternate cooking fuel in Haiti, where the reliance on wood has led to severe deforestation. Current production ...

  15. Design of a press for oil extraction from moringa seeds for Haiti

    E-Print Network [OSTI]

    Sabelli, Alessandra Maria, 1976-

    2004-01-01

    The project here presented focuses on the development of a harvesting tool for Haiti, a developing country, for the extraction of oil from the seeds of the moringa trees. Moringas have an extraordinarily nutritional potential ...

  16. Food Security in Haiti After the 2010 Earthquake: Analysis and Evaluation of NGO Relief Efforts 

    E-Print Network [OSTI]

    McGaughey, Katie

    Haiti suffered an earthquake in January of 2010, bringing instability and widespread hunger. Even after two years, many Haitians lack food security, and one must look at the failings of the Haitian government and those who attempted to provide aid...

  17. Managing growth of a non-profit healthcare supply chain in Haiti

    E-Print Network [OSTI]

    Heberley, Christine L. (Christine Laura)

    2010-01-01

    Partners in Health's (PIH) supply chain in Haiti has become strained over the past five years due to the organization's rapid growth. Under the current system, the majority of PIH's products are obtained through an annually ...

  18. Opportunity in Haiti : women as agents of resilience in post-disaster reconstruction

    E-Print Network [OSTI]

    Brickman Raredon, Anya

    2011-01-01

    The earthquake that struck Haiti on January 12, 2010, caused severe spatial and social disruption of many communities. Over the past sixteen months, as international and national institutions struggled to respond in an ...

  19. Foundations on Sand: An Analysis of the First U.S. Occupation of Haiti, 1915-1934

    E-Print Network [OSTI]

    Bunce, Peter L.

    2003-01-01

    in the Population . 48 Culture .. 49 Table 2: Haiti's Rulers Since Independence . 51 Part HI: The Never-ending Story... 54 Annexes 57 Annex A: The US Marine Corps'Military Campaigns in the First United States Occupation of Haiti 57 Appendix 1; First... Provisional Brigade of Marines 66 Appendix 2: Ships of the 1915 Haitian Campaign 74 Appendix 3: The Gendarmerie (Garde) d'Haïti, 1916-1934 75 Annex B: The Fiscal Case for Occupation 81 Appendix 1: Public Debt of Haiti, 1919 vs. 1922 93 vii Appendix 2...

  20. A7.0 magnitude earthquake devastated Haiti in January 2010. On March

    E-Print Network [OSTI]

    Bedwell, David M.

    A7.0 magnitude earthquake devastated Haiti in January 2010. On March 11, an 8.9 magnitude earthquake off the coast of Japan -- the most powerful in the nation's recorded history -- generated earthquake with an epicenter in northern Virginia rattled houses and nerves from Florida to Maine, damaging

  1. A comparison of perceived effectiveness of technology projects from viewpoints of external nongovernmental organizations and host country beneficiaries in Haiti 

    E-Print Network [OSTI]

    May, Donald Ray

    1998-01-01

    Three nongovernmental organizations sponsoring four projects in the Northern Department of Haiti were surveyed between May and July of 1998 to determine if their perceptions of the effectiveness of their projects correlated with the perceptions...

  2. Letter to the Editor : Rapidly-deployed small tent hospitals: lessons from the earthquake in Haiti.

    SciTech Connect (OSTI)

    Rosen, Y.; Gurman , P.; Verna, E.; Elman , N.; Labor, E. (Materials Science Division); (Superior NanoBioSystems LLC); (Fast Israeli Rescue & Search Team); (Clinique Adonai); (Mass. Inst. Tech.); (Univ. Haifa)

    2012-06-01

    The damage to medical facilities resulting form the January 2010 earthquake in haiti necessitated the establishment of field tent hospitals. Much of the local medical infrastructure was destroyed or limited operationally when the Fast Israel Rescue and Search Team (FIRST) arrived in Haiti shortly after the January 2010 earthquake. The FIRST deployed small tent hospitals in Port-au-Prince and in 11 remote areas outside of the city. Each tent was set up in less than a half hour. The tents were staffed with an orthopedic surgeon, gynecologists, primary care and emergency care physicians, a physician with previous experience in tropical medicine, nurses, paramedics, medics, and psychologists. The rapidly deployable and temporary nature of the effort allowed the team to treat and educate, as well as provide supplies for, thousands of refugees throughout Haiti. In addition, a local Haitian physician and his team created a small tent hospital to serve the Petion Refugee Camp and its environs. FIRST personnel also took shifts at this hospital.

  3. The role of ras signaling associative learning and memory in Drosophila

    E-Print Network [OSTI]

    Sakamoto, Toshimasa

    2004-01-01

    Ras is an evolutionally conserved signaling molecule that has been implicated in a variety of cellular events, such as cell proliferation, differentiation, and survival. Recent studies also suggest roles of Ras in neuronal ...

  4. Haiti: Feasibility of Waste-to-Energy Options at the Trutier Waste Site

    SciTech Connect (OSTI)

    Conrad, M. D.; Hunsberger, R.; Ness, J. E.; Harris, T.; Raibley, T.; Ursillo, P.

    2014-08-01

    This report provides further analysis of the feasibility of a waste-to-energy (WTE) facility in the area near Port-au-Prince, Haiti. NREL's previous analysis and reports identified anaerobic digestion (AD) as the optimal WTE technology at the facility. Building on the prior analyses, this report evaluates the conceptual financial and technical viability of implementing a combined waste management and electrical power production strategy by constructing a WTE facility at the existing Trutier waste site north of Port-au-Prince.

  5. Designing and building a peanut-shelling machine to help people in third world countries such as Ghana/Haiti

    E-Print Network [OSTI]

    Mohd Ramli, Rafiq H. (Rafiq Hidayat), 1980-

    2004-01-01

    A peanut shelling machine was designed and built to help improve the standard of living of people in third world countries such as Ghana and Haiti. Two prototypes were built to test the concept of using a wheel as a peanut ...

  6. Ras-GTP dimers activate the mitogen-activated protein kinase (MAPK) pathway

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nan, Xiaolin; Tamgüney, Tanja M.; Collisson, Eric A.; Lin, Li -Jung; Pitt, Cameron; Galeas, Jacqueline; Lewis, Sophia; Gray, Joe W.; McCormick, Frank; Chu, Steven

    2015-06-16

    Rat sarcoma (Ras) GTPases regulate cell proliferation and survival through effector pathways including Raf-MAPK, and are the most frequently mutated genes in human cancer. Although it is well established that Ras activity requires binding to both GTP and the membrane, details of how Ras operates on the cell membrane to activate its effectors remain elusive. Efforts to target mutant Ras in human cancers to therapeutic benefit have also been largely unsuccessful. Here we show that Ras-GTP forms dimers to activate MAPK. We used quantitative photoactivated localization microscopy (PALM) to analyze the nanoscale spatial organization of PAmCherry1-tagged KRas 4B (hereafter referredmore »to KRas) on the cell membrane under various signaling conditions. We found that at endogenous expression levels KRas forms dimers, and KRasG12D, a mutant that constitutively binds GTP, activates MAPK. Overexpression of KRas leads to formation of higher order Ras nanoclusters. Conversely, at lower expression levels, KRasG12D is monomeric and activates MAPK only when artificially dimerized. Moreover, dimerization and signaling of KRas are both dependent on an intact CAAX (C, cysteine; A, aliphatic; X, any amino acid) motif that is also known to mediate membrane localization. These results reveal a new, dimerization-dependent signaling mechanism of Ras, and suggest Ras dimers as a potential therapeutic target in mutant Ras-driven tumors.« less

  7. Ras Al Khaimah (RAK): World Oil Report 1991

    SciTech Connect (OSTI)

    Not Available

    1991-08-01

    This paper reports on Ras Al Khalmah (RAK) that had no drilling activity in 1990. In 1991, International Petroleum Ltd., (IPL), which holds a 1,100 sq mi concession onshore, may spud their West Jiri prospect. Amoco holds an offshore concession and was to have completed seismic last year. the RAK Gas Commission was reported to be operating the offshore Saleh gas field in 1990, which a former foreign concessionaire relinquished in mid-1989 since it had reached its economic limit. Production from Saleh last year is estimated at 1,000 bcpd and about 9 MMcfgd.

  8. An Active RFID Accountability System (RAS) for Constrained Wireless Environments

    SciTech Connect (OSTI)

    Barker, Alan M [ORNL; Hanson, Gregory R [ORNL; Sexton, Angela Kay [ORNL; Jones Jr, J P [ORNL; Freer, Eva B [ORNL; Sjoreen, Andrea L [ORNL

    2011-01-01

    A team from Oak Ridge National Laboratory (ORNL) has developed an RFID Accountability System (RAS) that allows items with active RFID tags to be tracked in environments where tags may not be able to transmit their location continuously. The system uses activators that transmit a short range signal. Active RFID tags are in a sleep state until they encounter an activator. Then they transmit a signal that is picked up by the antennas installed throughout the building. This paper presents the theory of operation, application areas, lessons learned, and key features developed over the course of seven years of development and use.

  9. 1. PMU Based RAS for a Hybrid AC/DC System 1.1. Project Team

    E-Print Network [OSTI]

    Li, Husheng

    1. PMU Based RAS for a Hybrid AC/DC System 1.1. Project Team Primary Thrust: Modeling Secondary to be detected more quickly allowing less severe action. This effort focuses on PMU driven response-based RAS from PMU units. The project goals break down into three efforts: · Investigating the possibility

  10. Effects of oncogenic Ras and p38 mitogen-activated protein kinase on the adhesion of normal human cells

    E-Print Network [OSTI]

    Waldman, Lynne K

    2010-01-01

    Activating mutations in RAS oncogenes commonly arise in human cancers. However, in experimental settings, oncogenic RAS has most often been studied at supraphysiological levels of expression. Importantly, work by others ...

  11. Selective Killing of K-ras Mutant Cancer Cells by Novel Small Molecule Inducers of Oxidative Stress

    E-Print Network [OSTI]

    Shaw, Alice

    Activating K-RAS mutations are the most frequent oncogenic mutations in human cancer. Numerous downstream signaling pathways have been shown to be deregulated by oncogenic K-ras. However, to date there are still no effective ...

  12. The RAS is a scholarship for students supported as research assistants who meet the eligibility requirements listed below. CHICAGO CAMPUS RESEARCH ASSISTANT SCHOLARSHIP (RAS)

    E-Print Network [OSTI]

    . University policy requires that all students receiving RAS be enrolled full time in The Graduate School (TGS) each quarter, either at full time residency 3-4 graduate units or at TGS 500. Please check that students have registered appropriately. (For the TGS 500 registration, make certain the student has already

  13. The nitric oxide-sensitive p21Ras-ERK pathway mediates S-nitrosoglutathione-induced apoptosis

    SciTech Connect (OSTI)

    Tsujita, Maristela [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil); Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo, SP (Brazil); Batista, Wagner L.; Ogata, Fernando T. [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil); Stern, Arnold [Department of Pharmacology, New York School of Medicine, New York University, New York (United States); Monteiro, Hugo P. [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil)], E-mail: hpmonte@uol.com.br; Arai, Roberto J. [Departamento de Bioquimica/Biologia Molecular, Centro Interdisciplinar de Terapia Genica CINTERGEN, Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Sao Paulo (Brazil)], E-mail: arairj@yahoo.com.br

    2008-05-16

    p21Ras protein plays a critical role in cellular signaling that induces either cell cycle progression or apoptosis. Nitric oxide (NO) has been consistently reported to activate p21Ras through the redox sensitive cysteine residue (118). In this study, we demonstrated that the p21Ras-ERK pathway regulates THP-1 monocyte/macrophage apoptosis induced by S-nitrosoglutathione (SNOG). This was apparent from studies in THP-1 cells expressing NO-insensitive p21Ras (p21Ras{sup C118S}) where the pro-apoptotic action of SNOG was almost abrogated. Three major MAP kinase pathways (ERK, JNK, and p38) that are downstream to p21Ras were investigated. It was observed that only the activation of ERK1/2 MAP kinases by SNOG in THP-1 cells was attributable to p21Ras. The inhibition of the ERK pathway by PD98059 markedly attenuated apoptosis in SNOG-treated THP-1 cells, but had a marginal effect on SNOG-treated THP-1 cells expressing NO-insensitive p21Ras. The inhibition of the JNK and p38 pathways by selective inhibitors had no marked effects on the percentage of apoptosis. The induction of p21Waf1 expression by SNOG was observed in THP-1 cells harboring mutant and wild-type p21Ras, however in cells expressing mutant Ras, the expression of p21Waf1 was significantly attenuated. The treatment of THP-1 cells expressing wild-type p21Ras with PD98059 resulted in significant attenuation of p21Waf1 expression. These results indicate that the redox sensitive p21Ras-ERK pathway plays a critical role in sensing and delivering the pro-apoptotic signaling mediated by SNOG.

  14. Qatargas exporting LNG from Qatar`s new Ras Laffan Port

    SciTech Connect (OSTI)

    1997-02-24

    When the 135,000 cu m LNG carrier Al Zubarah departed Ras Laffan Port in December, Qatar entered a new era of commerce that will both boost the emirate`s economic development and influence energy trade around the world. The event capped more than a decade of planning, design, and construction of Ras Laffan Port--the world`s newest and largest LNG exporting facility. During the 1980s, the focus in Qatar was on exploration and development of North field, which holds the world`s largest reserves of nonassociated natural gas. In the 1990s, efforts concentrated on establishing a direct production and export link between North field, the new multi-billion-dollar Qatar Liquefied Gas Co. (Qatargas) gas liquefaction plant at Ras Laffan, and LNG export facilities at the 8.5 sq km Ras Laffan Port. Markets of the Far East will be first to be served by LNG from Ras Laffan Port. Two 25-year LNG supply contracts have been signed with buyers in Japan and South Korea, and negotiations are under way with potential customers from China, Taiwan, and Thailand. The paper describes the port, its operations, and export projects.

  15. RAS Mutations Impact TNF-Induced Apoptosis in Colon Carcinoma Cells via ERK-Modulatory Negative and Positive Feedback Circuits along with non-ERK Pathway Effects

    E-Print Network [OSTI]

    Lauffenburger, Douglas A.

    More than 40% of colon cancers have a mutation in K-RAS or N-RAS, GTPases that operate as central hubs for multiple key signaling pathways within the cell. Utilizing an isogenic panel of colon carcinoma cells with K-RAS ...

  16. Small Molecule Screen Reveals Regulation of Survival Motor Neuron Protein Abundance by Ras Proteins

    E-Print Network [OSTI]

    Stockwell, Brent R.

    Small Molecule Screen Reveals Regulation of Survival Motor Neuron Protein Abundance by Ras Proteins States *S Supporting Information ABSTRACT: Small molecule modulators of protein activity have proven invaluable in the study of protein function and regulation. While inhibitors of protein activity

  17. Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching

    E-Print Network [OSTI]

    Kalyan C. Vinnakota; David A. Mitchell; Robert J. Deschenes; Tetsuro Wakatsuki; Daniel A. Beard

    2010-05-10

    Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with arbitrary bleach geometry and exchange of the membrane localized fluorophore with the cytosol during Fluorescence Recovery after Photobleaching (FRAP) experiments. The model simulations were used to design FRAP experiments with varying bleach region sizes on plasma-membrane localized wild type GFP-Ras2 with a dual lipid anchor and mutant GFP-Ras2C318S with a single lipid anchor in live yeast cells to investigate diffusional mobility and the presence of any exchange processes operating in the time scale of our experiments. Model parameters estimated using data from FRAP experiments with a 1 micron x 1 micron bleach region-of-interest (ROI) and a 0.5 micron x 0.5 micron bleach ROI showed that GFP-Ras2, single or dual lipid modified, diffuses as single species with no evidence of exchange with a cytoplasmic pool. This is the first report of Ras2 mobility in yeast plasma membrane. The methods developed in this study are generally applicable for studying diffusion and exchange of membrane associated fluorophores using FRAP on commercial confocal laser scanning microscopes.

  18. Effect of docosahexaenoic acid on ras post-translational processing and localization in a transgenic mouse colonic cell line 

    E-Print Network [OSTI]

    Collett, Esther Dick

    2000-01-01

    The acquisition of a chronically activated ras gene is a critical early step in colon cancer development, with membrane localization being a prerequisite for malignant transformation. Fish oil supplemented diets, containing docosahexaenoic acid (DHA...

  19. Structures of Ras Superfamily Effector Complexes: What have we learnt in two decades?

    E-Print Network [OSTI]

    Mott, Helen R.; Owen, Darerca

    2015-04-01

    . The smallest family is the Ran family, which contains a single member but being responsible for nuclear transport it is also one of the most abundant small G proteins in the cell. The Ras superfamily proteins can exist in two nucleotide-bound forms... different to that of PAK and WASP. ACK forms a more regular ?-sheet with Cdc42 and then proceeds to wrap around the body of Cdc42 making extensive contacts with switches 1 and 2 and forming an expansive binding interface. Unlike ACK and WASP, which...

  20. For the McCormick department, the RAS is intended only for PhD students. If a MS student will be funded from chart strings using the RAS tuition amounts, please contact the McCormick Research Administration Business

    E-Print Network [OSTI]

    that all students receiving RAS be enrolled full time in The Graduate School (TGS) per quarter, either for 3-4 graduate units or TGS 500. Please check that students have registered appropriately. (For the TGS 500 registration, make certain the student has already completed 8 quarters at the full-time rate

  1. For the McCormick department, the RAS is only for PhD students. If an MS student will be funded from chart strings using the RAS tuition amounts, please contact the McCormick Business Administrator, Paula Straaton

    E-Print Network [OSTI]

    that all students receiving RAS be enrolled full time in The Graduate School (TGS) each quarter, either for 3-4 graduate units or TGS 500. Please check that students have registered appropriately. (For the TGS 500 registration, make certain the student has already completed 8 quarters at the full-time rate

  2. RAS Mutations Affect Tumor Necrosis Factor–Induced Apoptosis in Colon Carcinoma Cells via ERK-Modulatory Negative and Positive Feedback Circuits Along with Non-ERK Pathway Effects

    E-Print Network [OSTI]

    Kreeger, Pamela K.

    More than 40% of colon cancers have a mutation in K-RAS or N-RAS, GTPases that operate as central hubs for multiple key signaling pathways within the cell. Utilizing an isogenic panel of colon carcinoma cells with K-RAS ...

  3. The C. elegans class A synthetic multivulva genes inhibit ectopic RAS-mediated vulval development by tightly restricting expression of lin-3 EGF

    E-Print Network [OSTI]

    Saffer, Adam M

    2011-01-01

    The class A and B synthetic multivulva (synMuv) genes of C. elegans redundantly antagonize an EGF/Ras pathway to prevent ectopic vulval induction. The class B synMuv genes encode many proteins known to remodel chromatin ...

  4. Results of measurements of an environment neutron background at BNO INR RAS objects with the helium proportional counter

    E-Print Network [OSTI]

    I. R. Barabanov; R. A. Etezov; Yu. M. Gavrilyuk; A. M. Gangapshev; A. M. Gezhaev; V. V. Kazalov; A. Kh. Khokonov; V. V. Kuzminov; S. I. Panasenko; S. S. Ratkevich

    2015-10-17

    A method of measurements of the environmental neutron background at the Baksan Neutrino Observatory of the INR RAS are described. Measurements were done by using of a proportional counter filled with mixture of Ar(2 at)+$^3$He(4 at). The results obtained at the surface and the underground laboratory of the BNO INR RAS are presented. It is shown that a neutron background in the underground laboratory at the 4900 m w.e. depth is decreased by $\\sim 260$ times without any special shield in a comparison with the Earth surface. A neutron flux density in the 5-1323.5~cm air height region is constant within the determination error and equal to $(7.1\\pm0.1_{\\rm{stat}}\\pm0.3_{\\rm{syst}})\\times10^{-3}$ s$^{-1}\\cdot$cm$^{-2}$.

  5. Results of measurements of an environment neutron background at BNO INR RAS objects with the helium proportional counter

    E-Print Network [OSTI]

    Barabanov, I R; Gavrilyuk, Yu M; Gangapshev, A M; Gezhaev, A M; Kazalov, V V; Khokonov, A Kh; Kuzminov, V V; Panasenko, S I; Ratkevich, S S

    2015-01-01

    A method of measurements of the environmental neutron background at the Baksan Neutrino Observatory of the INR RAS are described. Measurements were done by using of a proportional counter filled with mixture of Ar(2 at)+$^3$He(4 at). The results obtained at the surface and the underground laboratory of the BNO INR RAS are presented. It is shown that a neutron background in the underground laboratory at the 4900 m w.e. depth is decreased by $\\sim 260$ times without any special shield in a comparison with the Earth surface. A neutron flux density in the 5-1323.5~cm air height region is constant within the determination error and equal to $(7.1\\pm0.1_{\\rm{stat}}\\pm0.3_{\\rm{syst}})\\times10^{-3}$ s$^{-1}\\cdot$cm$^{-2}$.

  6. Haiti : the orphan chronicles

    E-Print Network [OSTI]

    Etnire, Michelle

    2000-01-01

    vents et marées'9 ("Give me your friendship and I will stand up against winds and tides") This little orphanage in the mountains above Fermathe does indeed cast a spell upon the visitor as does a sky-lit rainbow. The president and general director..., directed by Sr. Donna P. Bélizaire, stands high in the town of Kenscoff, a mountain district above Port-au-Prince. Although Sr. Donna was not present when I visited, there was another sister who gladly showed me around. Sr. Donna mostly resides abroad...

  7. Senescence-Associated Secretory Phenotypes Reveal Cell-Nonautonomous Functions of Oncogenic RAS and the p53 Tumor Suppressor

    SciTech Connect (OSTI)

    Coppé, Jean-Philippe; Patil, Christopher; Rodier, Francis; Sun, Yu; Munoz, Denise; Goldstein, Joshua; Nelson, Peter; Desprez, Pierre-Yves; Campisi, Judith

    2008-10-24

    Cellular senescence suppresses cancer by arresting cell proliferation, essentially permanently, in response to oncogenic stimuli, including genotoxic stress. We modified the use of antibody arrays to provide a quantitative assessment of factors secreted by senescent cells. We show that human cells induced to senesce by genotoxic stress secrete myriad factors associated with inflammation and malignancy. This senescence-associated secretory phenotype (SASP) developed slowly over several days and only after DNA damage of sufficient magnitude to induce senescence. Remarkably similar SASPs developed in normal fibroblasts, normal epithelial cells, and epithelial tumor cells after genotoxic stress in culture, and in epithelial tumor cells in vivo after treatment of prostate cancer patients with DNA-damaging chemotherapy. In cultured premalignant epithelial cells, SASPs induced an epithelial-mesenchyme transition and invasiveness, hallmarks of malignancy, by a paracrine mechanism that depended largely on the SASP factors interleukin (IL)-6 and IL-8. Strikingly, two manipulations markedly amplified, and accelerated development of, the SASPs: oncogenic RAS expression, which causes genotoxic stress and senescence in normal cells, and functional loss of the p53 tumor suppressor protein. Both loss of p53 and gain of oncogenic RAS also exacerbated the promalignant paracrine activities of the SASPs. Our findings define a central feature of genotoxic stress-induced senescence. Moreover, they suggest a cell-nonautonomous mechanism by which p53 can restrain, and oncogenic RAS can promote, the development of age-related cancer by altering the tissue microenvironment.

  8. Reduction of metastasis, cell invasion, and adhesion in mouse osteosarcoma by YM529/ONO-5920-induced blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathway

    SciTech Connect (OSTI)

    Tsubaki, Masanobu; Satou, Takao; Itoh, Tatsuki; Imano, Motohiro; Ogaki, Mitsuhiko; Yanae, Masashi; Depeartment of Pharmacy, Sakai Hospital, Kinki University School of Medicine, Sakai, Osaka 590-0132 ; Nishida, Shozo

    2012-03-15

    Osteosarcoma is one of the most common primary malignant bone tumors in children and adolescents. Some patients continue to have a poor prognosis, because of the metastatic disease. YM529/ONO-5920 is a nitrogen-containing bisphosphonate that has been used for the treatment of osteoporosis. YM529/ONO-5920 has recently been reported to induce apoptosis in various tumors including osteosarcoma. However, the mode of metastasis suppression in osteosarcoma by YM529/ONO-5920 is unclear. In the present study, we investigated whether YM529/ONO-5920 inhibited tumor cell migration, invasion, adhesion, or metastasis in the LM8 mouse osteosarcoma cell line. We found that YM529/ONO-5920 significantly inhibited metastasis, cell migration, invasion, and adhesion at concentrations that did not have antiproliferative effects on LM8 cells. YM529/ONO-5920 also inhibited the mRNA expression and protein activities of matrix metalloproteinases (MMPs). In addition, YM529/ONO-5920 suppressed phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and the serine/threonine protein kinase B (Akt) by the inhibition of Ras prenylation. Moreover, U0126, a mitogen-activated protein kinase kinase (MEK) 1/2 inhibitor, and LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, also inhibited LM8 cell migration, invasion, adhesion, and metastasis, as well as the mRNA expression and protein activities of MMP-1, MMP-2, MMP-9, and MT1-MMP. The results indicated that YM529/ONO-5920 suppressed the Ras/MEK/ERK and Ras/PI3K/Akt pathways, thereby inhibiting LM8 cell migration, invasion, adhesion, and metastasis. These findings suggest that YM529/ONO-5920 has potential clinical applications for the treatment of tumor cell metastasis in osteosarcoma. -- Highlights: ? We investigated whether YM529/ONO-5920 inhibited tumor metastasis in osteosarcoma. ? YM529/ONO-5920 inhibited metastasis, cell migration, invasion, and adhesion. ? YM529/ONO-5920 suppressed Ras signalings. ? YM529/ONO-5920 has potential clinical applications for the treatment in osteosarcoma.

  9. Activation of a c-K-ras oncogene by somatic mutation in mouse lymphomas induced by gamma radiation

    SciTech Connect (OSTI)

    Guerrero, I.; Villasante, A.; Corces, V.; Pellicer, A.

    1984-09-14

    Mouse tumors induced by gamma radiation are a useful model system for oncogenesis. DNA from such tumors contains an activated K-ras oncogene that can transform NIH 3T3 cells. This report describes the cloning of a fragment of the mouse K-ras oncogene containing the first exon from both a transformant in rat-2 cells and the brain of the same mouse that developed the tumor. Hybrid constructs containing one of the two pieces were made and only the plasmid including the first exon from the transformant gave rise to foci in NIH 3T3 cells. There was only a single base difference (G----A) in the exonic sequence, which changed glycine to aspartic acid in the transformant. By use of a synthetic oligonucleotide the presence of the mutation was demonstrated in the original tumor, ruling out modifications during DNA-mediated gene transfer and indicating that the alteration was present in the thymic lymphoma but absent from other nonmalignant tissue. The results are compatible with gamma radiation being a source of point mutations.

  10. Lead acetate induces EGFR activation upstream of SFK and PKC{alpha} linkage to the Ras/Raf-1/ERK signaling

    SciTech Connect (OSTI)

    Wang, C.-Y.; Wang, Y.-T.; Tzeng, D.-W. [Molecular Carcinogenesis Laboratory, Institute of Biotechnology and Department of Life Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan (China); Yang, J.-L. [Molecular Carcinogenesis Laboratory, Institute of Biotechnology and Department of Life Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan (China)], E-mail: jlyang@life.nthu.edu.tw

    2009-03-01

    Lead acetate (Pb), a probable human carcinogen, can activate protein kinase C (PKC) upstream of extracellular signal-regulated kinase 1 and 2 (ERK1/2). Yet, it remains unclear whether Pb activation of PKC {yields} ERK1/2 involves receptor/non-receptor tyrosine kinases and the Ras signaling transducer. Here we demonstrate a novel mechanism elicited by Pb for transmitting ERK1/2 signaling in CL3 human non-small-cell lung adenocarcinoma cells. Pb induction of higher steady-state levels of Ras-GTP was essential for increasing phospho-Raf-1{sup S338} and phospho-ERK1/2. Pre-treatment of the cells with a conventional PKC inhibitor Goe6976 or depleting PKC{alpha} using specific small interfering RNA blocked Pb induction of Ras-GTP. Pb also activated cellular tyrosine kinases. Specific pharmacological inhibitors, PD153035 for epidermal growth factor receptor (EGFR) and SU6656 for Src family tyrosine kinases (SFK), but not AG1296 for platelet-derived growth factor receptor, could suppress the Pb-induced tyrosine kinases, PKC{alpha}, Ras-GTP, phospho-Raf-1{sup S338} and phospho-ERK1/2. Furthermore, phosphorylation of tyrosines on the EGFR multiple autophosphorylation sites and the conserved SFK autophosphorylation site occurred during exposure of cells to Pb for 1-5 min and 5-30 min, respectively. Intriguingly, Pb activation of EGFR required the intrinsic kinase activity but not dimerization of the receptor. Inhibition of SFK or PKC{alpha} activities did not affect EGFR phosphorylation, while knockdown of EGFR blocked SFK phosphorylation and PKC{alpha} activation following Pb. Together, these results indicate that immediate activation of EGFR in response to Pb is obligatory for activation of SFK and PKC{alpha} and subsequent the Ras-Raf-1-MKK1/2-ERK1/2 signaling cascade.

  11. Haiti earthquake survivor to speak

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HABFESOpportunitiesNERSCGrid-based29Hai Ah Nam Hai Ah Nam-The power

  12. Extracellular signal-regulated kinase 2 (ERK-2) mediated phosphorylation regulates nucleo-cytoplasmic shuttling and cell growth control of Ras-associated tumor suppressor protein, RASSF2

    SciTech Connect (OSTI)

    Kumari, Gita [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India)] [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India); Mahalingam, S., E-mail: mahalingam@iitm.ac.in [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India); Department of Biotechnology, Laboratory of Molecular Virology and Cell Biology, Indian Institute of Technology-Madras, Chennai 600 036 (India)

    2009-10-01

    Ras GTPase controls the normal cell growth through binding with an array of effector molecules, such as Raf and PI3-kinase in a GTP-dependent manner. RASSF2, a member of the Ras association domain family, is known to be involved in the suppression of cell growth and is frequently down-regulated in various tumor tissues by promoter hypermethylation. In the present study, we demonstrate that RASSF2 shuttles between nucleus and cytoplasm by a signal-mediated process and its export from the nucleus is sensitive to leptomycin B. Amino acids between 240 to 260 in the C-terminus of RASSF2 harbor a functional nuclear export signal (NES), which is necessary and sufficient for efficient export of RASSF2 from the nucleus. Substitution of conserved Ile254, Val257 and Leu259 within the minimal NES impaired RASSF2 export from the nucleus. In addition, wild type but not the nuclear export defective RASSF2 mutant interacts with export receptor, CRM-1 and exported from the nucleus. Surprisingly, we observed nucleolar localization for the nuclear export defective mutant suggesting the possibility that RASSF2 may localize in different cellular compartments transiently in a cell cycle dependent manner and the observed nuclear localization for wild type protein may be due to faster export kinetics from the nucleolus. Furthermore, our data suggest that RASSF2 is specifically phosphorylated by MAPK/ERK-2 and the inhibitors of MAPK pathway impair the phosphorylation and subsequently block the export of RASSF2 from the nucleus. These data clearly suggest that ERK-2 mediated phosphorylation plays an important role in regulating the nucleo-cytoplasmic shuttling of RASSF2. Interestingly, nuclear import defective mutant of RASSF2 failed to induce cell cycle arrest at G1/S phase and apoptosis suggesting that RASSF2 regulates cell growth in a nuclear localization dependent manner. Collectively, these data provided evidence for the first time that MAPK/ERK-2 mediated phosphorylation regulates nucleo-cytoplasmic transport and cell growth arrest activity of RASSF2. Taken together, the present study suggests that active transport between nucleus and cytoplasm may constitute an important regulatory mechanism for RASSF2 function.

  13. CCD polarimetry of distant comets C/2010 S1 (LINEAR) and C/2010 R1 (LINEAR) at the 6-m telescope of the SAO RAS

    E-Print Network [OSTI]

    Ivanova, Oleksandra V; Afanasiev, Viktor L; Reshetnyk, Volodymyr M; Korsun, Pavlo P

    2015-01-01

    We present first measurements of the degree of linear polarization of distant comets C/2010 S1 (LINEAR) and C/2010 R1 (LINEAR) at heliocentric distances r= 5.9 - 7.0 AU. Observations were carried out with the SCORPIO-2 focal reducer at the 6-m telescope of the SAO RAS. Both comets showed considerable level of activity beyond a zone where water ice sublimation is negligible (up to 5 AU). Significant spatial variations both in the intensity and polarization are found in both comets. The slope of radial profiles of intensity changes gradually with the distance from the photocenter: from - 0.7 near the nucleus up to about - 1.3 for larger distances (up to 100000 km). The variation in polarization profiles indicates the non uniformity in the polarization distribution over the coma. The polarization degree over the coma gradually increases (in absolute value) with increasing the photocentric distance from of about - 1.9% up to - 3% for comet C/2010 S1 (LINEAR), and from of about - 2.5% up to - 3.5% for comet C/2010...

  14. Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras–Raf–ERK–Sp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: •Nestin expression in C6 glioma cells is induced by FGF-2. •Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. •The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. •The mRNA of FGFR1 and 3 are detected in C6 glioma cells. •Ras–Raf–ERK–Sp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFR–MAPK–ERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  15. come dream with us. . . Home >> Haiti Reborn

    E-Print Network [OSTI]

    matrixes, forms of dealing with nature and water, means of consumption, culture of daily life toward self destruction that the powerful have chosen, given the lack of equitable possibilities

  16. Haiti: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    lack of investment in the energy infrastructure, caused in part by the fact that prices are artificially held below the true cost of production and delivery. Power supply is...

  17. Haiti-NREL Cooperation | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County,SolarFERCInformation 3.1 -HachijojimaHaddam,GreenNREL

  18. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  19. Predicting soil erosion from Cap Rouge Plateau, Haiti 

    E-Print Network [OSTI]

    Marcelin, Fritz Sauveur

    1985-01-01

    in waterways, causing more frequent flooding, higher costs for navigation, and reduced storage capacity of reservoirs. Geological (sometimes called natural or normal) erosion is the inexorable and continuous process of evolution of the earth's surface... clays and other soil grains together in quite stable forms in many strongly leached, temperate- region soils and in numerous tropical soils. These soils may be quite resistant to erosion. 27 Cro in Hi Soils just plowed from native vegetation...

  20. Volunteers hope ORNL technology will speed Haiti's long quake recovery

    E-Print Network [OSTI]

    -Battelle Scholarship . . . . . . . . . . 3 ORNL donations saved damaged clinic . . . . . . . . . . . . . . . . . 3

  1. Land Tenure and Reform in Haiti Amber Bethell

    E-Print Network [OSTI]

    Onsrud, Harlan J.

    struggles with poverty and environmental degradation. The political system is far from stable. Most Haitians per year. Many theorize that securing tenure in land is one method for combating poverty and hunger

  2. Haiti-Facility for Environmentally Friendly Transport Technology...

    Open Energy Info (EERE)

    fr Internationale Zusammenarbeit (GIZ) GmbH Sector Climate Focus Area Renewable Energy Topics Adaptation, Low emission development planning Website http:...

  3. Haiti-Caribbean Community (CARICOM) Sustainable Energy Roadmap and Strategy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County,SolarFERCInformation 3.1 -HachijojimaHaddam,Green

  4. Haiti-Regional Implementation Plan for CARICOM's Climate Change

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County,SolarFERCInformation 3.1Resilience Framework | Open

  5. Haiti-Facility for Environmentally Friendly Transport Technology and

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA JumpDuimen River Power Co LtdGuntherGreen PowerHipoo

  6. Haiti-IAEA Energy Planning | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA JumpDuimen River Power Co LtdGuntherGreen PowerHipooIAEA Energy

  7. Haiti - Annual Average Wind Speed at 80 meters

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journalvivo Low-Dose Low34 Revision 0August 9, 74° 73° 72° 20° "

  8. In her RAS Presidential Address for 2005, Kathryn A Whaler

    E-Print Network [OSTI]

    away from power lines and buildings with elec- trical power (be it generator or mains supplied, and wind. There- fore equipment must be deployed in quiet areas ­ both electromagnetically, so keeping well to reduce wind noise, but this does not reduce wind-caused tree-root vibration. Away from the main

  9. INSTITUTE OF APPLIED PHYSICS RAS RUSSIA NIZHNY NOVGOROD

    E-Print Network [OSTI]

    Fominov, Yakov

    ) The common features of electromagnetic pulses dynamics were investigated in the case of Kerr type nonlinearity. The common features of electromagnetic pulses dynamics were investigated in the case of Kerr type from the area occupied by electromagnetic laser pulse could be neglected quasi-flat model

  10. Aberrant Ras/MAPK signaling in skeletal development

    E-Print Network [OSTI]

    Nedelcu, Simona

    2013-01-01

    The Mitogen-activated protein kinase(MAPK) signaling pathway has been studied intensively in the context of neoplastic transformation. Other studies have focused on the roles of this pathway during development and have ...

  11. High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic

    E-Print Network [OSTI]

    Gire, Stephen

    AbstractBackgroundWhole-genome sequencing is an important tool for understanding microbial evolution and identifying the emergence of functionally important variants over the course of epidemics. In October 2010, a severe ...

  12. The Haiti Earthquake: Disaster Lessons and Response from an Emergency Medicine Perspective

    E-Print Network [OSTI]

    Lee, Sharon; Tenny, Montessa

    2010-01-01

    solution” Dr. Koenig notes. ACEP’s policy advises againstcreating safety issues. The ACEP section on Disaster

  13. Analysis for the Design of a Sustainable Housing Complex in Haiti 

    E-Print Network [OSTI]

    Dinka, M.; Cho, S.

    2010-01-01

    , and garden irrigation use[27]. As a result it conserves the potable water supply. Typically these systems consist of gutters, downspouts, and storage containers. Greywater Systems: Greywater refers to the reuse of water drained from baths, showers...

  14. Set in Haiti: The Construction of Race in Historia de una bala de plata

    E-Print Network [OSTI]

    Kuhnheim, Jill S.

    1996-04-01

    invocation of the imaginary, puts race relations right on the surface creating an exemplary focus on the issues by "estranging the signifying process" (Elam 18). With this opening in mind, then, I was surprised at the results when, teaching the play, I... Christophe. Paris: Presence Africaine, 1963. Elam, Keir. The Semiotics of Theatre and Drama. London and New York: Methuen, 1980. Foner, Philip S. Paul Robeson Speaks: Writings, Speeches, interviews 1918-1974. New York: Brunner/Mazel Publishers, 1978...

  15. www.latinlawyer.com hen Haiti was devastated by an earthquake in January,

    E-Print Network [OSTI]

    Shyu, Mei-Ling

    power plant projects worldwide. "Those are heavy-duty negotiations because you're starting an entire power plant," says Guillermo Levy, a former corporate associate at Hunton & Williams who joined,Caterpillar Power Generation Systems, based in Houston, sells industrial gas turbine engines for use in turnkey

  16. DIGGING THE ROOTS or Resistance and identity politics of the mouvman rasin in Haiti

    E-Print Network [OSTI]

    Jayaram, Kiran C.

    2002-01-01

    , wherefrom they created a new music, mizik rasin. An analysis of the sanba-s’ politics of identity regarding history, music, dress, language, and religion conveys a cultural critique of late Duvalierism and other contemporary social issues....

  17. Performance of Charcoal Cookstoves for Haiti Part 1: Results from the Water Boiling Test

    E-Print Network [OSTI]

    Booker, Kayje

    2012-01-01

    is brought to a boil. 3. Simmer (low power): Immediatelywater is maintained at a simmer for 45 minutes. In this60% open during the low power (simmer phase) test. The same

  18. Solar-driven humidification dehumidification desalination for potable use in Haiti

    E-Print Network [OSTI]

    Liburd, Shannon Omari

    2010-01-01

    Worldwide water scarcity, especially in the developing world, provides the impetus for utilizing inexpensive desalination technologies on a wider scale to contribute to freshwater supply. Small-scale desalination technologies, ...

  19. Haiti-Pilot Program for Climate Resilience (PPCR) | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County,SolarFERCInformation 3.1

  20. Haiti-Designing and Communicating Low Carbon Energy Roadmaps for Small

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View NewGuam: Energy Resources Jump to:Wind

  1. Haiti-Low-Carbon Energy Roadmaps for the Greater Antilles | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View NewGuam: Energy Resources Jump to:WindInformation Low-Carbon

  2. The RAS is a scholarship for students supported as research assistants who meet the eligibility requirements listed below. CHICAGO CAMPUS RESEARCH ASSISTANT SCHOLARSHIP (RAS)

    E-Print Network [OSTI]

    in The Graduate School (TGS) per quarter, either at full time residency 3-4 graduate units or at TGS 500. Please check that students have registered appropriately. (For the TGS 500 registration, make certain the student has already completed 8 quarters at the full-time rate exclusive of any previous TGS 512

  3. Children Are the Wealth of the Poor: Prevention of Mother-to-Child Transmission of HIV in Haiti

    E-Print Network [OSTI]

    Denney, Rachel

    2012-08-31

    with men (Maternowska 2006). It is socially acceptable for men to have children with multiple partners, as a man’s virility is valued more than his fidelity to a single partner. This sort of polygamy is not considered the same as adultery. It is public...

  4. --RIT captures Haiti disaster with high-tech imaging system --In the aftermath of the Jan. 12 earthquake that struck

    E-Print Network [OSTI]

    Rogers, John A.

    in the crowd. They're... -- Harnessing the divas of the nanoworld -- Boron nitride nanotubes (BNNTs a 3.5x optical zoom, takes sharp images, is inexpensive to make and is only the size of a nickel. (A

  5. OAiC RiDGE NATIONAL LABORAl-ORY LKCKKBSP HAITI MANA%ED AND OPERATED BY

    Office of Legacy Management (LM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal Gas &SCE-SessionsSouthReport for the Weldon Spring,7=cr5rnP 7694 i+lJNew York,' ,92 7%

  6. RAS Framework Prototype Real-time Data Reduction of Monitoring Data

    E-Print Network [OSTI]

    Engelmann, Christian

    System Management Node Compute Node Monitor Runtime Environment Application Resource Manager Migration Management Node Compute Node Filter Monitor Runtime Environment Application Resource Manager Migration System Management Node Compute Node Analysis Filter Monitor Runtime Environment Application Resource

  7. Network analysis of differential Ras isoform mutation effects on intestinal epithelial responses to TNF-?

    E-Print Network [OSTI]

    Lau, Ken S.

    Tumor necrosis factor alpha (TNF-?) is an inflammatory cytokine that can elicit distinct cellular behaviors under different molecular contexts. Mitogen activated protein kinase (MAPK) pathways, especially the extracellular ...

  8. FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in C. elegans

    E-Print Network [OSTI]

    Lu, Jiamiao; Dentler, William L., Jr; Lundquist, Erik A.

    2008-05-16

    of rachis formation and germ line organization are not well understood. Results: Mutations in the fli-1 gene disrupt rachis organization without affecting meiotic differentiation, a phenotype in C. elegans referred to here as the g erm l ine m...

  9. Anti-tumour activity in RAS-driven tumours by blocking AKT and MEK

    E-Print Network [OSTI]

    Tolcher, Anthony W.; Khan, Khurum; Ong, Michael; Banerji, Udai; Papadimitrakopoulou, Vassiliki; Gandara, David; Patnaik, Amita; Baird, Richard D.; Olmos, David; Garrett, Christopher R.; Skolnik, Jeffrey M.; Rubin, Eric; Smith, Paul; Huang, Pearl; Learoyd, Maria; Shannon, Keith; Morosky, Anne; Tetteh, Ernestina; Jou, Ying-Ming; Papadopoulos, Kyriakos P.; Moreno, Victor; Kaiser, Brianne; Yap, Timothy A.; Yan, Li; de Bono, Johann S.

    2014-12-16

    retinal pigment epithelium detachment. There were no meaningful pharmacokinetic drug-drug interactions. Clinical anti-tumour activity included RECIST 1.0-confirmed partial responses in non-small cell lung cancer and low-grade ovarian carcinoma...

  10. Institutions of the Russian Academy of Sciences Joint Institute for High Temperatures RAS

    E-Print Network [OSTI]

    Fominov, Yakov

    and temperatures, low-temperature plasma physics, issues of physics and power engineering, as well as technology of z-pinch dis

  11. Proceedings of the 8th Brazilian Symposium in Information and Human Language Technology, pages 194198, Cuiaba, MT, Brazil, October 2426, 2011. c 2011 Sociedade Brasileira de Computac~ao

    E-Print Network [OSTI]

    composição e descrição de um corpus sobre o terremoto do Haiti em 2010 com apoio do pacote Natural Language Toolkit (NLTK). 1. Introdução O terremoto ocorrido no Haiti em 12 de janeiro de 2010 demonstra

  12. Improvement of kiln design and combustion/carbonization timing to produce charcoal from agricultural waste in Developing countries

    E-Print Network [OSTI]

    Martinez, Jason A. (Jason Alexander)

    2006-01-01

    Current economic conditions in third world countries like Haiti are so poor that the majority of the population has no access to energy sources that people in the first world take for granted. In Haiti the last two percent ...

  13. A comparative analysis of emissions from bagasse charcoal and wood charcoal

    E-Print Network [OSTI]

    Ramírez, Andrés, 1982-

    2005-01-01

    Haiti is the poorest country in the Western hemisphere and is in need of cheap cooking fuel source. Currently, lump charcoal, the cooking fuel of Haiti, is made by carbonizing trees in ditches before selling the charcoal ...

  14. Supplementary materials of "High-resolution back-projection at regional distance: application to the Haiti M7.0 earthquake and comparisons with finite source studies"

    E-Print Network [OSTI]

    Ampuero, Jean Paul

    of the synthetic seismograms of the mainshock filtered from 0.2 to 0.7 Hz, assuming a point source with mechanism given by the CMT solution. Fig. S2 Fit of the teleseismic P and SH waveforms for the kinematic slip joint kinematic slip model. The P and SH waveforms are sorted with increasing azimuth angle (number

  15. in this issuewe report on proposed changes at MITMedical (see interview below); an update on faculty and student response to the earthquake in Haiti (page 4);

    E-Print Network [OSTI]

    de Weck, Olivier L.

    into public view. With on the order of 20,000 active nuclear war- heads deployed on planes, submarines Opportunities Toward Nuclear Disarmament: Reviving Faculty Roles? TH E F OLLOWI N G D I S CU S S I ON between. The opening of the UN Review Conference on Non- Proliferation of Nuclear Weapons also brings the issue

  16. Izvestiya:M athematics 64:3 439--485 c #20 0 0 RAS(DoM) and LMS

    E-Print Network [OSTI]

    Shirikyan, Armen

    Foundation for Basic Research (grant no. 97­0 1­0 0 541) and the Volkswagen Foundation (grant no. I­710 16

  17. Senescence-Associated Secretory Phenotypes Reveal Cell-Nonautonomous Functions of Oncogenic RAS and the p53 Tumor Suppressor

    E-Print Network [OSTI]

    Coppé, Jean-Philippe

    2009-01-01

    some components of the DDR are important for establishingis not a simple or classic DDR. Rather, the SASP is a slowCM, conditioned medium; DDR, DNA damage response; ELISA,

  18. Determining the optimal river gauge location for a flood early warning system in Uganda using HEC-RAS and AHP

    E-Print Network [OSTI]

    Cheung, Joyce, M. Eng. Massachusetts Institute of Technology

    2014-01-01

    Flooding of the Manafwa River in Eastern Uganda causes significant damage in the district of Butaleja, and often occurs without advance warning. In 2012, the American Red Cross in Uganda requested MIT to develop a flood ...

  19. ORIGINAL ARTICLE B-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase

    E-Print Network [OSTI]

    ERK1 and ERK2 (extracellular signal-regulated kinases 1 and 2) are probably the best studied and best understood of the mitogen-activated protein kinases (MAPKs). ERK1 and ERK2 respond to diverse stimuli activators are the kinases MEK1 and MEK2 (for MAPK kinase or ERK kinase), which phosphorylate ERK1 and ERK2

  20. Energy Transition Initiative: Island Energy Snapshot - Dominican Republic

    SciTech Connect (OSTI)

    2015-09-01

    This profile provides a snapshot of the energy landscape of the Dominican Republic, a Caribbean nation that shares the island of Hispaniola with Haiti to the west.

  1. A Soldier at Heart: The Life of Smedley Butler, 1881-1940

    E-Print Network [OSTI]

    Myers, Eric Dennis

    2012-01-01

    during his 1938 congressional testimony is any indication,in Haiti in testimony before a congressional committee inhis two-day testimony in front of a congressional committee

  2. Uganda Manafwa River early flood warning system development hydrologic watershed modeling using HEC-HMS, HEC-RAS, ArcGIS

    E-Print Network [OSTI]

    Ma, Yan, M. Eng. Massachusetts Institute of Technology

    2013-01-01

    The Manafwa River basin spans several districts in Eastern Uganda. Over the years, frequent floods have constantly posed a great threat to the local communities in these districts. The Uganda Red Cross Society (URCS) intends ...

  3. Dietary, lifestyle and clinicopathological factors associated with BRAF and K-ras mutations arising in distinct subsets of colorectal cancers in the EPIC Norfolk study

    E-Print Network [OSTI]

    Naguib, Adam; Mitrou, Panagiota N.; Gay, Laura J.; Cooke, James C.; Luben, Robert; Ball, Richard Y.; McTaggart, Alison; Arends, Mark J.; Rodwell, Sheila A.

    2010-03-16

    (8):1137-1144. 41. Soreide K, Slewa A, Stokkeland PJ, van Diermen B, Janssen EA, Soreide JA, Baak JP, Korner H: Microsatellite instability and DNA ploidy in colorectal cancer: potential implications for patients undergoing systematic surveillance after resection...

  4. Tissue-specific interactions between oncogenic K-ras and the p19A?r?f?_p53 pathway determine susceptibility to transformation

    E-Print Network [OSTI]

    Young, Nathan Price

    2010-01-01

    Tumor development is a multi-step process driven by the collective action of gain-of-function mutations in oncogenes and loss-of-function alterations in tumor suppressor genes. The particular spectrum of mutations in a ...

  5. The pro-apoptotic K-Ras 4A proto-oncoprotein does not affect tumorigenesis in the ApcMin/+ mouse small intestine

    E-Print Network [OSTI]

    Patek, Charles E.; Arends, Mark J.; Rose, Lorraine; Luo, Feijun; Walker, Marion; Devenney, Paul S.; Berry, Rachel L.; Lawrence, Nicola J.; Ridgway, Rachel A.; Sansom, Owen J.; Hooper, Martin L.

    2008-06-13

    -actin transcripts were 185 bp, 158 bp and 148 bp respectively. The specificities of the PCR reactions were confirmed by dissociation curve analysis and 2% agarose gel electrophoresis. All PCR products were analysed when in the exponential phase of PCR amplification... of uncensored observations. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0 50 100 150 200 250 300 350 400 Age (days) K ap la n- M ei er s ur vi va l f un ct io nPage 6 of 9 (page number not for citation purposes) complex relationship between apoptosis and cancer...

  6. The effect of sociocultural factors on the quality of relations of the United States with Hispaniola: analysis of the American military occupation of Haiti and the Dominican Republic (1915-1934) 

    E-Print Network [OSTI]

    Alvarez, Fausto B.

    1988-01-01

    with Hispaniola reveals a strong emphasis on economic and strategic interests (see, for example, Langley, 1983' Wiarda and Kryzanek, 1982; Langley, 1982; Lowenthal, 1972, 1969; Knight, 1970). Therefore, the sociological significance of this study... with the role of social and cultural factors involved in the application of this policy. Melvin Knight, an American historian who had first-hand acquaintance with and who wrote about the military occupation of Hispaniola, described this "dilemma" as follows...

  7. Pierre Y. Julien Hong Kong -December 2004

    E-Print Network [OSTI]

    Julien, Pierre Y.

    Development in Haiti #12;2 List the Pros-cons of this Dam Advantages: · · · · Disadvantages: · · Demographic - Discussion Objectives Impact of Hydropower Development 1. Case-Study in Haiti on the role of dams; 2. Discussion on the role of dams and future developments; 3. Class participation is expected. Hydropower

  8. INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS Int. J. Numer. Meth. Fluids 2003; 43:895902 Prepared using fldauth.cls [Version: 2002/09/18 v1.01

    E-Print Network [OSTI]

    Farrell, Paul A.

    Institute of Mathematics & Mechanics, Ural Branch of RAS, Ekaterinburg, Russia SUMMARY In this paper we

  9. Journal of Solid State Chemistry 179 (2006) 10931099 High-temperature phase equilibria in the oxide systems

    E-Print Network [OSTI]

    Poeppelmeier, Kenneth R.

    2006-01-01

    State Chemistry, Ural Division of RAS, #91Pervomaiskaia Str., GSP-145, 620219 Ekaterinburg, Russia b

  10. Stanley Hall Safety Committee Agenda 11:00 am 12:00 noon, Wednesday, March 10, 2010

    E-Print Network [OSTI]

    Doudna, Jennifer A.

    Stanley Hall [Safety Committee and DSC Comments in Green] 1. Earthquakes in Haiti and Chile All Popcorn smoke from microwave; February ­ Tea maker steam in smoke detector Liquid Nitrogen Fill

  11. Florida Atlantic University Division of Research

    E-Print Network [OSTI]

    Fernandez, Eduardo

    -rays or lasers? Yes No Does the proposal include boating and diving? Yes No Does the proposal include hazardous, Cuba, Cyprus, Haiti, Indonesia, Iran, Iraq, Ivory Coast, Liberia, Libya, N. Korea, Rwanda, Somalia

  12. Three Essays On Agricultural and Forestry Offsets In Climate Change Mitigation 

    E-Print Network [OSTI]

    Feng, Siyi

    2012-07-16

    , New Caledonia, Papua New Guinea, Samoa, Solomon Islands, Tonga, Vanuatu 17 RCAM Antigua Barbuda, Bahamas, Barbados, Belize, Bermuda, Costa Rica, Cuba, Dominica, Dominican Republic, El Salvador, Grenada, Guadeloupe, Guatemala, Haiti, Honduras...

  13. Design of a crushing and agglomeration process for manufacturing bagasse charcoal

    E-Print Network [OSTI]

    Fan, Victoria Y. (Victoria Yue-May)

    2006-01-01

    In Haiti, wood and wood charcoal are common fuels for cooking. This practice has contributed to deforestation, leading to erosion and fatal floods. The availability of charcoal made from a different source other than wood, ...

  14. Novel Application of Metering Pump on Diesel Aftertreatment

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Guyan a The Bahamas Cuba Dominican Republic Panama Costa Rica Nicaragu a Honduras Guatemala El Salv ador T rinidad and Tobag o Jam. Haiti Puerto Rico (US) Greenland (Den.)...

  15. Support to Biofuels in Latin America and the Caribbean

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    biofuels - El Salvador (APEX-funded) - Dominican Republic - DR (APEX-funded) - Haiti, Guatemala and Honduras (IDB-funded) - DR Phase II to evaluate specific projects (IDB-funded) -...

  16. Strategic relational communication in crisis : the humanitarian example

    E-Print Network [OSTI]

    Michaels, Olufunke

    2013-01-01

    The discourse on Haiti is both vast and varied with public attention soaring when an earthquake hit the island in January 2010. Many questions have since been raised by global stakeholders as to how the situation was ...

  17. On the ac magnetic susceptibility of spin-chains: solitons in one-dimensional systems

    E-Print Network [OSTI]

    Fominov, Yakov

    University, Krakow, Poland E. Pikina Institute for Problems of Oil and Gas RAS, Moscow, Russia W. Haase Shubnikov' Institute of Crystallography RAS, Moscow, Russia Z. Tomkowicz Institute of Physics, Jagiellonian

  18. Using a Risk-informed Approach to Find a Solution for Spillway Deficiencies with Upstream

    E-Print Network [OSTI]

    Bowles, David S.

    · Reclamation for Portfolio Risk Mgt ~ 1995 · UK for Portfolio RAs ~ 2000 ­ Justify dam safety CAPEX in utility

  19. ON AMALGAMATION OF REDUCTS OF POLYADIC ALGEBRAS TAREK SAYED AHMED

    E-Print Network [OSTI]

    for classes of algeb* *ras rele- vant to algebraic logic. Amalgamation is interesting from a purely algebraic

  20. Approximation of the solution and its derivative for the singularly perturbed Black-Scholes equation

    E-Print Network [OSTI]

    Stephan, Frank

    Institute of Mathematics and Mechanics, Ural Division of RAS, Yekaterinburg, Russia 1 #12;For the problem (1

  1. Playing 'Sticky Hands' With A Humanoid Robot

    E-Print Network [OSTI]

    Hale, J.G.

    Hale,J.G. Pollick,F.E. First IEEE-RAS International Conference on Humanoid Robots (Humanoids 2000) IEEE

  2. 10.1101/gad.7.4.621Access the most recent version at doi: 1993 7: 621-632Genes Dev.

    E-Print Network [OSTI]

    Perrimon, Norbert

    fate determination p21ras/Rasl, an essential component torso signaling in Drosophila Xiangyi Lu, 1 Tze

  3. Differentiation Self-renewal

    E-Print Network [OSTI]

    Glyde, Henry R.

    Off state Ras Ras­GAP PI3K ERK1 ERK2 Differentiation SC1 SC1 Self-renewal Ras On state GDP GTP N NN the kinase enzyme Figure 1 | Chemically induced stem-cell self-renewal. Chen et al.2 have discovered, which promotes stem-cell self-renewal. Activated Ras also switches on the enzymes ERK1 and ERK2, which

  4. Provability logics with quantifiers on proofs Rostislav E. Yavorsky \\Lambda

    E-Print Network [OSTI]

    Yavorskiy, Rostislav

    ­3752, fax (095) 135­0555 rey@mi.ras.ru Abstract We study here extensions of the Artemov's logic of proofs

  5. Advanced Design and Commissioning Tools for Energy-Efficient Building Technologies

    E-Print Network [OSTI]

    Bauman, Fred; Webster, Tom; Zhang, Hui; Arens, Ed

    2012-01-01

    for generating thermal stratification. Detailed hardware andstratification (RAS), (2) underfloor plenum air leakage, and (3) thermalthermal decay is increased which tends to work against increased stratification.

  6. Pawangs on the Malay Frontier: Miraculous Intermediaries of Rice, Ore, Beasts and Guns

    E-Print Network [OSTI]

    Sevea, Terenjit Singh

    2013-01-01

    The Graves of Tarim: Genealogy and Mobility across theHo, The Graves of Tarim: Genealogy and Mobility across theposthumous, Hadhrami genealogy, as an Alawi sayyid. 237 RAS

  7. Bacterial Water Contamination: Associated Risk Factors and Beliefs in a Rural Community in Northern Evan Torline

    E-Print Network [OSTI]

    Zhou, Yaoqi

    Bacterial Water Contamination: Associated Risk Factors and Beliefs in a Rural Community in Northern of its residents are living without access to clean drinking water. This simple issue causes terrible community of Mol, Haiti before the implementation of a chlorine water filtration system in it. A handful

  8. Spectres of Multiplicity. 18th -Century Literature Revisited from the Outside in , in Christie

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    portrayed as dreaming an enduring dream of unity. A great deal of administrative and intellectual energy from China, spoons made from Peruvian iron, and sugar cultivated in Haiti by slaves dragged out-French departments, the "Grand Design" of political unification dreamt by Henry IV and his minister Sully (revived

  9. Web MSNBC Search Alerts Newsletters RSS Help MSN Home Hotmail Sign In Conflict in Iraq

    E-Print Network [OSTI]

    3,000 deaths, the vast majority in Haiti, and $45 billion in damage in the United States alone, the United States among them, have chosen France as the site for a $13 billion experimental nuclear fusion by Japan, the United States, South Korea, Russia, China and the European Union, but the six parties had

  10. Fine-Grained Remote Monitoring, Control and Pre-Paid Electrical Service in Rural Microgrids

    E-Print Network [OSTI]

    Rowe, Anthony

    Fine-Grained Remote Monitoring, Control and Pre-Paid Electrical Service in Rural Microgrids Maxim microgrid deployment in rural Les Anglais, Haiti. The system consists of a three-tiered architecture of faults like short circuits and downed lines within microgrids. Keywords--Microgrid, Deployment Experience

  11. CRC handbook of agricultural energy potential of developing countries. Volume I

    SciTech Connect (OSTI)

    Duke, J.A.

    1986-01-01

    The contents of this book are: Introduction, Argentina, Bangladesh, Benin, Bolivia, Botswana, Bourkina (Upper Volta), Brazil, Burma, Burundi, Cameroon, Chad, Chile, Columbia, Costa Rica, Djibouti, Dominican Republic, Ecuador, El Salvador, Ethiopia, French Guiana, Gambia, Ghana, Guatemala, Guinea, Guyana, Haiti, Honduras, India, Indonesia, Jamaica, Appendix I. Conventional and Energetic Yields, Appendix II, Phytomass Files, and References.

  12. Geography 1: UCLA Spring 2002, Prof. Smith. Name:____________________Section___________ EXERCISE 1: GEOGRAPHIC GRID, TIME, AND EARTH-SUN RELATIONSHIPS

    E-Print Network [OSTI]

    ____________ ____________ c. Mexico City, Mexico ____________ ____________ d. Port-au-Prince, Haiti? ___________________________ 3. When the solar time in Madras, India (13°N, 80°E) is 3:00 pm, what is the solar time in Los Angeles, CA, USA (32°N, 118°W)? (show calculation) ___________________________ 4. It is solar noon at 135

  13. Geography 1: UCLA Fall 2000, Prof. Smith. Name:____________________Section___________ EXERCISE 1: GEOGRAPHIC GRID, TIME, AND EARTH-SUN RELATIONSHIPS

    E-Print Network [OSTI]

    ____________ ____________ c. Mexico City, Mexico ____________ ____________ d. Port-au-Prince, Haiti? ___________________________ 3. When the solar time in Madras, India (13°N, 80°E) is 3:00 pm, what is the solar time in Los Angeles, CA, USA (32°N, 118°W)? (show calculation) ___________________________ 4. It is solar noon at 135

  14. 2015, Latin American Public Opinion Project www.AmericasBarometer.org

    E-Print Network [OSTI]

    couples. In recent years, samesex marriages have been recognized in Uruguay, Brazil Argentina Uruguay Haiti Belize Trinidad and Tobago Suriname Dominican Republic Panama Peru Mexico Brazil United States Canada 0.0 20.0 40.0 60.0 80.0 Approval of Same-Sex Couples' Right to Marry 95 % Confidence

  15. www.outreach.vt.edu 20102011 Issue

    E-Print Network [OSTI]

    Buehrer, R. Michael

    experience virginia tech students feel the transformative power of studying abroad 20 a New high-tech to the earth in haiti a virginia tech team creates grains of hope in the central Plateau 6 Green Business Ideas takes Six stories from the virginia tech com- munity show impact on people's lives 25 viva virginia

  16. Paul Conal Winters Department of Economics, American University

    E-Print Network [OSTI]

    Carlini, David

    , Washington, DC Provided technical support for conducting impact evaluations of Bank-funded projects including, Department for Mexico, Central America, Dominican Republic and Haiti, Environment and Natural Resources of the United Nations, Economic and Social Division, Rome, Italy Study leave from the University of New England

  17. V I R G I N I A P O L Y T E C H N I C I N S T I T U T E A N D S T A T E U N I V E R S I T Y An e q u al o p p ort un it y, af fi rma t iv e ac ti o n in st it u ti on

    E-Print Network [OSTI]

    want to thank everyone who has been involved in various aspects of preparing for the SACS review over hundred participants to The Inn at Virginia Tech last month. The commitment of our faculty and graduate as Hokies United in response to the earthquake in Haiti. Virginia Tech has strong involvement in multiple

  18. International Journal of Computer Science and Applications, Technomathematics Research Foundation

    E-Print Network [OSTI]

    Hexmoor, Henry

    to prediction and early warning systems. Poorly managed disasters are observed as the consequence for many years. Disasters such as the tsunami in 2004 and hurricane Katrina in 2005 provided opportunities the Haiti earthquake in 2010, a "cluster" system that the #12;Henry Hexmoor and Deepti Shetty2 United

  19. Call for Papers In 2011, the Food and Agriculture Organization published a book: `Save and Grow'. The challenge

    E-Print Network [OSTI]

    Stephens, Graeme L.

    agriculture production systems (CAPS) being developed in several regions of the world. We are seeking papers and FAO CA researches in several regions of the world Haiti project CIRAD team FAO team · CANSEA) · Special night program possibly at Lake Ton Le Sap December 12: Cambodian Women and Men Farmers experiences

  20. Creating a Pathway to Sustainability IIT Wanger Institute for Sustainable Energy Research

    E-Print Network [OSTI]

    Heller, Barbara

    ............................................................................................................5 Clean Coal Technology Production of Methane from Hydrates and Unconventional Sources Biofuels of the Future Demonstrates IIT Vision Engineering a Clean Water Supply in Haiti Community Outreach Education IIT first developed a formal affiliation with the Institute of Gas Technology (IGT), establishing

  1. UNITED STATES DEPARTMENT OF THE INTERIOR Harold L. Ickes, Secretary

    E-Print Network [OSTI]

    to Haiti, Cuba, and central Mexico. Thus it migrates across both of our international boundaries, Biologist in Charge, Distribution and Migration of Birds, Division of Wildlife Research, Fish andWildlife Service CONTENTS Page Introduction.- ,.. i Distribution 1 Breeding range 1 Winter range _ 2 Migration 2

  2. Solidarity Immersion 2015 Human Rights in the Global Economy

    E-Print Network [OSTI]

    Rock, Chris

    Solidarity Immersion 2015 Human Rights in the Global Economy Dominican Republic July 30th - August the Dominican Republic and Haiti for an up-close look at how our global economy and fight for change is woven-hand understanding of the bigger picture of human rights in the global economy through direct testimony from workers

  3. Anti-inflammatory Properties of Cowpea Phenotypes with different Phenolic Profiles 

    E-Print Network [OSTI]

    Ojwang, Leonnard

    2012-07-16

    of protein. Brazil, India, Myanmar, Sri Lanka, Haiti, Senegal, Cameron, Burkina Faso, Australia and the U.S. also produce significant quantities (18, 19). In the U.S., the total production of cowpeas is estimated at 60,000 to 80,000 acres, of which...

  4. Tuberculosis among Recent US

    E-Print Network [OSTI]

    Cohen, Ted

    and assumed that these populations were depleted only by death (no emigration from the United States immigrants by time since arrival in the United States from each of 6 countries of origin: China, Haiti, India country of origin, age at diagnosis, and years since arrival in the United States (Centers for Disease

  5. JournalofCellScience Two distinct functions for PI3-kinases in

    E-Print Network [OSTI]

    Weiner, Orion

    derived from the Ras-binding domain of PI3K1 suggests that Ras is activated throughout vesicle formation and macropinocytosis, where their role and specific mode of regulation are less clear. The role of PI3-kinase in chemotaxis ­ the movement of cells along chemical gradients ­ has been the subject of major debate

  6. RUSSIAN ACADEMY OF SCIENCES Ural Branch

    E-Print Network [OSTI]

    Turova, Varvara

    with the homicidal chau#11;eur dynamics: Scienti#12;c reports. IMM Ural Branch of RAS. Ekaterinburg, Russia, 2000RUSSIAN ACADEMY OF SCIENCES Ural Branch Institute of Mathematics and Mechanics Scienti#12;c reports of Mathematics and Mechanics Ural Branch of RAS Patsko V.S., Turova V.L. Numerical study of di#11;erential games

  7. Feb. 11, 2008 Advanced Fault Tolerance Solutions for High Performance Computing 1/47 Advanced Fault Tolerance Solutions

    E-Print Network [OSTI]

    Engelmann, Christian

    Feb. 11, 2008 Advanced Fault Tolerance Solutions for High Performance Computing 1/47 RAS RAS Advanced Fault Tolerance Solutions for High Performance Computing Christian Engelmann Oak Ridge National Solutions for High Performance Computing 2/47 · Nation's largest energy laboratory · Nation's largest

  8. The Dictyostelium Mitogen-activated Protein Kinase ERK2

    E-Print Network [OSTI]

    Devreotes, Peter

    The Dictyostelium Mitogen- activated Protein Kinase ERK2 Is Regulated by Ras and cAMP- dependent kinase ERK2 activity (1). In this study we show that other pathways required for aggregation, including Ras and cAMP-dependent protein kinase (PKA), are impor- tant regulators of ERK2 activation

  9. Grid Induction -2nd hands on workshop

    E-Print Network [OSTI]

    Beimel, Amos

    acquainted with the latest versions of LCG infrastructure, Gilda and its Genius portal. Come and learn how in Spain IHEP in Russia II-SAS in Slovakia IMPB RAS in Russia INFN in Italy INTA in Spain ITEP in Russia JINR in Russia JSI in Slovenia KFKI RMKI in Hungary KIAM RAS in Russia KTH in Sweden KU

  10. Adv. Geosci., 8, 3338, 2006 www.adv-geosci.net/8/33/2006/

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    under a Creative Commons License. Advances in Geosciences Web portal on environmental sciences "ATMOS" E., Tomsk, 634055 Russia 2Institute for Numerical Mathematics RAS, 8 Gubkina Str., Moscow, 119991 Russia 3Institute of Atmospheric Optics SB RAS, 1 Akademicheskii Ave.,Tomsk, 634055 Russia Received: 15 September

  11. Influence of interlayer interaction on tunnelling disordered two-dimensional electron systems

    E-Print Network [OSTI]

    Fominov, Yakov

    systems Institute of Microelectronics Technology RAS, Chernogolovka, Russia #12;IMT RASIMT RAS Yu.V. Dubrovskii E.E. Vdovin L. Eaves P.C. Main O.N. Makarovskii M. Henini V.A.Volkov J.-C. Portal G. Hill #12

  12. The (Re)Construction of Realities in Systems Biology The Jacobian Way

    E-Print Network [OSTI]

    Rostock, Universität

    P MEK P ERK P P ERK P P P P Gene Elk SAP SUR-8SUR-8 KsrKsr MP1MP1 Hsp90Hsp90 14-3-3 ReceptorReceptor Growth factors receptor Ras kinase Raf P P P P MEK P ERK P P Growth factors receptor RasRas kinase Raf P P P P MEK P ERK P P Raf P P P P MEK P ERK P P ERK P P P P ERK P P ERK P P PP PP Gene Elk SAP Gene

  13. Shrimp Imports 37 Kenya 0.6 in 1972 Total 223.2 38 Mozambique 33.2

    E-Print Network [OSTI]

    .3 The statistics, provided to the NMFS 14 Greenland 117.6 47 Paraguay 46.0 Statistics and Market News Division countries with 21 Austria 3.8 54 Surinam 2,132.6 more than 80 million pounds. India was 22 Turkey 7.5 55- 25 India 33,523.5 58 Haiti 12.2 ports. Imports from the People's Repub- 26 Sri Lamka 489.4 59 Jamaica

  14. Haitian-English Dictionary

    E-Print Network [OSTI]

    Freeman, Bryant C.

    2004-01-01

    little or no lexical similarities. The term kreyol, used almost universally within Haiti, is no more justifiable when speaking English than would be the term français to refer to French or Deutsch to German. And instead of the hybrid term "Haitian.... xii Of course this official enactment simply formalized currents long in preparation, evolving from a humble status as the language of informal discourse, to the realm of formal discourse — eloquently illustrated by its exclusive use at the very...

  15. South America, Central America, the Caribbean, and Mexico

    SciTech Connect (OSTI)

    Deal, C.

    1981-10-01

    Summaries of oil and gas drillings, well completions, production, exploratory wells, exploration activity and wildcat drilling were given for South America, Central America, the Caribbean, and Mexico. The countries, islands, etc. included Argentina, Bahamas, Barbados, Belize, Bolivia, Brazil, Colombia, Costa Rica, Cuba, Dominican Republic, Ecuador, French Guiana, Guatemala, Guyana, Haiti, Honduras, Jamaica, Leeward and Windward Islands, Mexico, Netherlands Antilles, Nicaragua, Panama, Paraguay, Peru, Puerto Rico, El Salvador, Surinam, Trinidad and Venezuela. 16 figures, 120 tables. (DP)

  16. Organization charts of the Haitian judiciary and military

    E-Print Network [OSTI]

    1995-01-01

    Occasional Paper N° 9 Organization Charts of the Haitian Judiciary and Military Institute of Haitian Studies University of Kansas Occasional Paper N° 9 Bryant C. Freeman, Ph.D. General Editor Organization Charts of the Haitian Judiciary... Tel.: (913) 864-4431 Occasional Paper N° 9 May 1995 ORGANIZATION CHARTS OF THE HAITIAN JUDICIARY AND MILITARY This is the ninth in a series of documents concerning Haiti to be made more readily available through the University of Kansas Institute...

  17. Information Systems 28 (2003) 241242 Special issue on data management in bioinformatics

    E-Print Network [OSTI]

    Zaki, Mohammed Javeed

    2003-01-01

    , discusses the application of the array DBMS, RasDaMan, to the management of gene expression data in situ biological data, namely, the inherent complexity of biological data, domain knowledge barrier, the evolution

  18. HER receptor-mediated dynamic signalling in breast cancer cells 

    E-Print Network [OSTI]

    Hu, Huizhong

    2011-07-05

    The dynamics of cell signalling are critical to cell fate decisions. Human Epidermal growth factor Receptors (HERs)-mediated Ras/Raf/MEK/ERK and PI3K/Akt signalling cascades relay extracellular signals from the plasma ...

  19. JOURNAL DE PHYSIQUE C o l l o q u e C I O , s u p p l m e n t au n012, Tome 46, d c e m b r e 1985 page CIO-139

    E-Print Network [OSTI]

    Boyer, Edmond

    the sample and the temperature of the solution ras maintained by a heat exchanger placed in a reservoirHz) by a Marx type (3) composite oscillator of 112 rsvelength in a torsional mode of oscillation. The internal

  20. ORIGINAL PAPER Julia A. Bahteeva Ilia A. Leonidov

    E-Print Network [OSTI]

    Poeppelmeier, Kenneth R.

    . V. Patrakeev E. B. Mitberg Æ V. L. Kozhevnikov (&) Institute of Solid State Chemistry, Ural Branch of RAS, Pervomaiskaia 91, 620219 Ekaterinburg, Russia E-mail: kozhevnikov@imp.uran.ru Tel.: +7

  1. Atmos. Chem. Phys., 14, 98079830, 2014 www.atmos-chem-phys.net/14/9807/2014/

    E-Print Network [OSTI]

    Institute of Natural Science, Ural Federal University, Ekaterinburg, Russia 3Laboratoire de Météorologie Ecology UB RAS, Ekaterinburg, Russia Correspondence to: V. Gryazin (victor.gryazin@gmail.com) Received: 14

  2. Invariant-based Cryptosystems and Their Security Against Provable

    E-Print Network [OSTI]

    Grigoriev, Dima

    of Mathematics 27 Fontanka, 191023 St.Petersburg, Russia http://logic.pdmi.ras.ru/arist/ http based on some nat- ural complexity assumption like P=NP. It is likely to be impossible (see [BT03

  3. Journal of Solid State Chemistry 179 (2006) 30453051 Oxygen-ion and electron conductivity in Sr2(Fe1xGax)2O5

    E-Print Network [OSTI]

    Poeppelmeier, Kenneth R.

    2006-01-01

    .R. Poeppelmeierc , V.L. Kozhevnikova,Ã a Institute of Solid State Chemistry, Ural Branch of RAS, Pervomaiskaia 91, Ekaterinburg 620219, Russia b Moscow State University, Vorob'evy gory, Moscow 119987, Russia c Department

  4. Identification of conserved chromatin-regulatory complexes among the class B synthetic multivulva proteins

    E-Print Network [OSTI]

    Harrison, Melissa M

    2006-01-01

    The class A, B, and C synthetic Multivulva (synMuv) genes act redundantly to antagonize Ras-mediated vulval induction in C. elegans. Many of these genes encode proteins that are likely to function in transcriptional ...

  5. MAPK pathway: a role in development, disease and behaviour 

    E-Print Network [OSTI]

    Anastasaki, Korina

    2011-11-25

    Mutations in the RAS-RAF-MEK-ERK (MAPK) pathway give rise to a range of developmental disorders collectively referred to as the RASopathies. De novo germline mutations in patients suffering from these syndromes promote ...

  6. NT13 Tuesday Posters 2 The field emission peculiarities of nanodiode with

    E-Print Network [OSTI]

    Maruyama, Shigeo

    , Russia (german_kos@mail.ru) 2 Institute of Nanotechnology of Microelectronics of RAS, Moscow, Russia. Artemenko, P. P. Aseev, D. S. Shapiro, JETP Lett., 87 (2008) 692 One-dimensional N2 gas inside single

  7. Viruses and cancer

    SciTech Connect (OSTI)

    Rigby, P.W.J.; Wilkie, N.M.

    1985-01-01

    This book contains 14 selections. Some of the titles are: Immortalising gene(s) encoded by Epstein-Barr Virus; Adenovirus genes involved in transformation. What determines the oncogenic phenotype.; Oncogenesis by mouse mammary tumour virus; and Transforming ras genes.

  8. Feasibility of Wholesale Electricity Competition in a Developing Country: Insights from Simulating a Market in Maharashtra State, India

    E-Print Network [OSTI]

    Phadke, Amol

    2007-01-01

    India looking at 2 mt more LNG from RasGas”. August 29 thhas planned for major LNG imports. Minimum flow constraintsbasis. Liquefied Natural Gas (LNG) Prices India is currently

  9. Breaking a Pocket of Resistance in the Fight Against Cancer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Breaking a Pocket of Resistance in the Fight Against Cancer Breaking a Pocket of Resistance in the Fight Against Cancer Print Thursday, 12 December 2013 11:55 ras protein The new...

  10. Flood Routing on Small Streams: A Review of Muskingum-Cunge, Cascading Reservoirs, and Full Dynamic Solutions

    E-Print Network [OSTI]

    Heatherman, William Joseph

    2008-01-01

    dynamic" solutions: the UNET-based solver in HEC-RAS and the National Weather Service's FLDWAV program. The four models were tested on four natural streams in northeastern Kansas. Detailed procedures for creating "equivalent reaches" were developed...

  11. Peripartum Cardiomyopathy: Predictors of Recovery

    E-Print Network [OSTI]

    Pillarisetti, Jayasree

    2013-08-31

    significant geo-demographic variation from 1 in 500 live births in Haiti to 1 in 4000 live births in the USA (3 -6). Identified risk factors for PPCM include multiparity, advanced maternal age, twins, preeclampsia, gestational hypertension and African... 9 3 3 3 7 4 1 4 5 4 9 5 3 5 7 6 1 6 5 6 9 7 3 7 7 8 1 8 5 8 9 9 3 9 7 w ee k s Time to diagnosis of peripartum cardiomyopathy p o st p ar tu m an te p ar tu m Patients 5 Maternal and Fetal Characteristics: Preeclampsia was diagnosed...

  12. Institutions and the Volatility Curse

    E-Print Network [OSTI]

    Leong, Weishu; Mohaddes, Kamiar

    2011-07-10

    and 12 can be found in the Middle East and North Africa (MENA) region. Our sample also includes 32 out of the 34 OECD countries and 8 out of the 12 of the Organization of the Petroleum Exporting Countries (OPEC). Thus our sample is very comprehensive... -Bissau Netherlands Tanzania Cameroon Guyana New Zealand Thailand Canada Haiti Nicaragua Togo Central African Rep. Honduras Niger Trinidad and Tobago Chad Hungary Nigeria Tunisia Chile India Norway Turkey China, People?s Rep. of Indonesia Oman Uganda Colombia Iran, I...

  13. 1

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  14. 1

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  15. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquake survivor torobot

  16. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquake survivor

  17. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquake survivorforum

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  19. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquake

  20. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquakemission laser tool

  1. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquakemission laser

  2. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquakemission

  3. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti earthquakemissionPreliminary

  4. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaiti

  5. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree to settle

  6. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree to settleusing

  7. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree to

  8. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree tomodel brain

  9. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree tomodel

  10. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree tomodelImagine

  11. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agree

  12. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties agreeBehr-Andres

  13. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiParties

  14. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesand NNSS team to

  15. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesand NNSS team towind

  16. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesand NNSS team

  17. 1

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  18. 1

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  19. 1

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  20. 1

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  1. 1

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  2. 1

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  3. 1

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  4. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesandwin two

  5. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesandwin twospinoff

  6. 1

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    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesandwin

  7. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesandwinlibrary project

  8. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservationBio-InspiredAtmospheric RadiationMeltwater effectsHaitiPartiesandwinlibrary

  9. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

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  10. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

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  11. Cutting-Balloon Angioplasty in Transplant Renal Artery Stenosis as First-Line Treatment in the Early Postoperative Period

    SciTech Connect (OSTI)

    Ucar, Adem; Yahyayev, Aghakishi; Bakkaloglu, Huseyin; Agayev, Ayaz; Aydin, Ali Emin; Rozanes, Izzet

    2011-02-15

    Percutaneous transluminal angioplasty has been successfully used for the treatment of transplant renal artery stenosis (RAS). Cutting-balloon angioplasty (CBA) is being used as a second option in pressure-resistant stenosis. It is thought that CBA is less traumatic and therefore restenosis occurs less frequently than in conventional angioplasty. This case report describes the unusual use of a cutting balloon in transplant RAS as a first option in the early postoperative period. Long-term follow-up data are also presented.

  12. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Lenore Rasmussen, David Schramm, Lewis D. Meixler, Charles A. Gentile, George Ascione, Carl Tilson, and Kelsey Pagdon

    2010-02-19

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple, and now contract (new development) with low electric input. In addition, Ras Labs produces EAP materials that quickly contract and expand, repeatedly, by reversing the polarity of the electric input. These recent developments are important attributes in the field of electroactivity because of the ability of contraction and contraction-expansion to produce biomimetric motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to determine the mechanisms during contraction of these EAPs.

  13. Posgrado en Direccin y Consultora en Nuevas

    E-Print Network [OSTI]

    Yannuzzi Sanchez, Marcelo

    que permite medir el trabajo que deben realizar los alumnos para la adquisi- ción de los conocimientos, capacidades, y competencias necesarias para superar las diferentes materias de su plan de estudios. Cada ECTS equivale a 25 horas de estu- dio e incluye el tiempo dedicado a las ho- ras lectivas, horas de trabajo del

  14. November 13, 2003 COS Preship Review 1Resolution Specification Definition of resolution

    E-Print Network [OSTI]

    Colorado at Boulder, University of

    November 13, 2003 COS Preship Review 1Resolution Specification · Definition of resolution ­ In all be measured in the COS science data after extracting a one-dimensional spetrum from the two-dimensional raw or other resoluti Sys A t 2/03 Test, COS Sys T/V Calibration w/ RAS/Cal, Plan, XXXX, ?/XX/02. Report? BATC

  15. Crawford,Hapgood:UK space exploration A&G December 2007 Vol. 48 6.9

    E-Print Network [OSTI]

    Crawford, Ian

    and benefits of levels of involvement in space exploration in the future. Many RAS Fellows work in fields, China, India and Korea) as a risk. The argument is that con- tinuing such disproportionate engagement at risk. Clearly these issues are not of direct concern to the Society, but they matter indirectly. Our

  16. Multi-way Hierarchic Classification of Musical Instrument Sounds Alicja A. Wieczorkowska

    E-Print Network [OSTI]

    Ras, Zbigniew W.

    Multi-way Hierarchic Classification of Musical Instrument Sounds Alicja A. Wieczorkowska Polish 28223, USA ras@uncc.edu Abstract Musical instrument sounds can be classified in various ways, depending on the instrument or articulation classifi- cation. This paper reviews a number of possible general- izations

  17. Real-time management of berth allocation with stochastic arrival and handling times

    E-Print Network [OSTI]

    Bierlaire, Michel

    of Architecture, Civil and Environmental Engineer- ing (ENAC), Ecole Polytechnique Fédérale de Lausanne (EPFL), CH Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA, alerera@isye.gatech.edu 1 #12 by the SAQR port, Ras Al Khaimah, UAE, where the berthing plans are regularly disrupted owing to a high degree

  18. RESIDENT ARCHIVE SERVICES OF THE YOHKOH LEGACY DATA *, L Acton1

    E-Print Network [OSTI]

    McKenzie, David E.

    Email: takeda@solar.physics.montana.edu 2 Lockheed-Martin Solar and Astrophysics Laboratory, 3251 group of the Resident Archives (RAs) selected for funding for NASA's Virtual Observatories for Heliophysics Data program. YLA provides the best corrected data set of solar X-ray images and spectra from

  19. On the Power of Randomized Branching Farid Ablayev \\Lambda Marek Karpinski y

    E-Print Network [OSTI]

    Behnke, Sven

    supported by the Volkswagen­Stiftung and the Basic Research Grant 96­ 01­01962 Email: ablayev@ksu.ras.ru y, and by the Volkswagen­Stiftung. Email: marek@cs.uni­bonn.de 1 #12; [6]). In the paper we define the notion

  20. Pilot Scale Study of Excess Sludge Production Reduction in Wastewater Treatment by Ozone

    E-Print Network [OSTI]

    Barthelat, Francois

    Pilot Scale Study of Excess Sludge Production Reduction in Wastewater Treatment by Ozone Yuan Ma-scale reactors were operated at the LaPrairie Wastewater Treatment plant (one control and one ozonated) to investigate the sludge reduction potential of partially ozonating sludge return activated sludge (RAS

  1. Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene

    E-Print Network [OSTI]

    that activation of the ERK and phos- phatidylinositol 3-kinase (PI3K) signaling path- ways cooperate to transform T (LT) and small t (ST) oncoproteins, and an activated allele of H-RAS (H-RASV12 ) render a wide range exhibited by human epi- thelial cancers complicates efforts to identify mutations critical for malignant

  2. FOB Undergoing Test and Alignment in FAS at Ball Aerospace OPTICAL TESTS AND ALIGNMENTS

    E-Print Network [OSTI]

    Schneider, Glenn

    FOB Undergoing Test and Alignment in FAS at Ball Aerospace OPTICAL TESTS AND ALIGNMENTS After the FOB alignment is complete, and it passes environmental tests, it will be checked out in a second HST. The NICMOS FOB will relay simulated images from RAS/HOMS to a Photometrics CCD camera placed where the three

  3. FOURFOUR--QUADRANT POWER SUPPLIESQUADRANT POWER SUPPLIES FOR STEERING ELECTROMAGNETSFOR STEERING ELECTROMAGNETS

    E-Print Network [OSTI]

    Kozak, Victor R.

    -3 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - DIPOLE CORRECTORS MULTI-POLE CORRECTORS W500P kW2P Cooling: Air Natural IUP = V100U,A6I V100U,A25I PANEL #12;O. Belikov, RuPAC 2008, Zvenigorod, October 1 BINP SB RASBINP SB RAS O

  4. Trends and uncertainties in Siberian indicators of 20th century warming

    E-Print Network [OSTI]

    Esper, Jan

    , 3012 Bern, Switzerland, zLaboratory of Dendrochronology, Institute of Plant and Animal Ecology, Ural Branch of Russian Academy of Sciences, 8 Marta Street, 222, Ekaterinburg 620144, Russia, §V. N. Sukachev Institute of Forest SB RAS, 660036 Krasnoyarsk, Akademgorodok, Russia Abstract Estimates of past climate

  5. Mechanical force generation by G proteins Ioan Kosztin*

    E-Print Network [OSTI]

    Kosztin, Ioan

    Mechanical force generation by G proteins Ioan Kosztin* , Robijn Bruinsma§¶ , Paul O' Lague-hydrolyzing G proteins are molecular switches that play a critical role in cell signaling processes. Here we use molecular dynamics simulations to show that Ras, a monomeric G protein, can generate mechanical force upon

  6. Graduate Research Assistant Workload Agreement Research Assistant: _____________________________________________

    E-Print Network [OSTI]

    Northern British Columbia, University of

    Graduate Research Assistant Workload Agreement Research Assistant by the supervisor(s). Purpose of Research Assistantship The primary purpose of Research Assistantships is to (a) act graduate students. The secondary goals of awarding RAs are to expose students to current research

  7. Parallel Query Support for Multidimensional Data: Inter-object Parallelism

    E-Print Network [OSTI]

    Baumann, Peter

    array database management systems. It discusses their implementation in the RasDaMan DBMS, the first DBMS for generic mul- tidimensional array data. The efficiency of the techniques presented is demon) into Data- base Management Systems (DBMS) has received growing attention. Current scientific contributions

  8. BOSQUE REACH ARROYO DE LAS CAAS TO SOUTH BOUNDARY BOSQUE DEL

    E-Print Network [OSTI]

    Julien, Pierre Y.

    flow discharges varying in increments of 200 cfs from 0 cfs up to 5,000 cfs. To better understand over time and location. The HEC-RAS model produced a low overbank flow value of 1,000 cfs and 800 cfs

  9. FREE ELECTRON LASER FOR SIBERIAN CENTRE FOR PHOTOCHEMICAL RESEARCH: THE CONTROL SYSTEM FOR THE

    E-Print Network [OSTI]

    Kozak, Victor R.

    FREE ELECTRON LASER FOR SIBERIAN CENTRE FOR PHOTOCHEMICAL RESEARCH: THE CONTROL SYSTEM of the Siberian Branch of RAS. Abstract. A control system for the magnetic system of the free electron laser (FEL) is described. The characteristics and structure of the power supply system are presented. The control system

  10. Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process

    E-Print Network [OSTI]

    Gilmore, Stephen

    Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process algebra) on the Extracellular signal Regulated Kinase (ERK) signalling pathway [1] through modelling in a Markovian process choices. The system which we consider is the Ras/Raf-1/MEK/ERK signalling path- way, as presented in [1

  11. Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process

    E-Print Network [OSTI]

    Calder, Muffy

    Modelling the influence of RKIP on the ERK signalling pathway using the stochastic process algebra Regulated Kinase (ERK) signalling pathway [5] through modelling in a Markovian process algebra, PEPA [11 durations and probabilistic choices. The system which we consider is the Ras/Raf-1/MEK/ERK signalling

  12. BioCONCUR 2004 Preliminary Version Modelling the influence of RKIP on the ERK

    E-Print Network [OSTI]

    Calder, Muffy

    Bio­CONCUR 2004 Preliminary Version Modelling the influence of RKIP on the ERK signalling pathway Protein (RKIP) on the Extracellular signal Regulated Kinase (ERK) signalling pathway [1] through modelling stochastic durations and probabilistic choices. The system which we consider is the Ras/Raf­1/MEK/ERK

  13. Multiple ERK substrates execute single biological processes in Caenorhabditis elegans

    E-Print Network [OSTI]

    Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ, 2008) RAS-extracellular signal regulated kinase (ERK) signaling governs multiple aspects of cell fate. Understanding how perturbations to the ERK signaling pathway lead to developmental disorders and cancer hinges

  14. Erk1 and Erk2 Regulate Endothelial Cell Proliferation and Migration during Mouse Embryonic Angiogenesis

    E-Print Network [OSTI]

    Erk1 and Erk2 Regulate Endothelial Cell Proliferation and Migration during Mouse Embryonic membrane with subsequent migration and proliferation of endothelial cells. The Ras/ Raf/MEK/ERK pathway is required for EC function during angiogenesis. Although in vitro studies implicate ERK1 and ERK2

  15. Spred2 is involved in imatinib-induced cytotoxicity in chronic myeloid leukemia cells

    SciTech Connect (OSTI)

    Liu, Xiao-Yun; Yang, Yue-Feng; Wu, Chu-Tse; Xiao, Feng-Jun; Zhang, Qun-Wei [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Ma, Xiao-Ni [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Li, Qing-Fang; Yan, Jun [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Wang, Hua, E-mail: wanghualjh@gmail.com [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Wang, Li-Sheng, E-mail: wangls@nic.bmi.ac.cn [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)

    2010-03-19

    Spreds, a recently established class of negative regulators of the Ras-ERK (extracellular signal-regulated kinase) pathway, are involved in hematogenesises, allergic disorders and tumourigenesis. However, their role in hematologic neoplasms is largely unknown. Possible effects of Spreds on other signal pathways closely related to Ras-ERK have been poorly investigated. In this study, we investigated the in vitro effects of Spred2 on chronic myeloid leukemia (CML) cells. In addition to inhibiting the well-established Ras-ERK cascade, adenovirus-mediated Spred2 over-expression inhibits constitutive and stem cell factor (SCF)-stimulated sphingosine kinase-1 (SPHK1) and Mcl-1 expression, as well as inhibiting proliferation and inducing apoptosis in CML cells. In K562 cells and primary CML cells, imatinib induces endogenous Spred2 expression. Spred2 silencing by stable RNA interference partly protects K562 cells against imatinib-induced apoptosis. Together, these data implicate Spred2 in imatinib-induced cytotoxicity in CML cells, possibly by inhibiting the Ras-ERK cascade and the pro-survival signaling molecules SPHK1 and Mcl-1. These findings reveal potential targets for selective therapy of CML.

  16. Role of Protein Kinase C in the Signal Pathways That Link Na /K -ATPase to ERK1/2*

    E-Print Network [OSTI]

    Askari, Amir

    Role of Protein Kinase C in the Signal Pathways That Link Na /K -ATPase to ERK1/2* Received, transactivation of EGFR, and activation of the Ras/ ERK1/2 cascade. To determine the role of protein kinase C (PKC, but not toxic, effects of ouabain in rat cardiac ventricles. Ouabain-induced activation of ERK1/2 was blocked

  17. Developmental Cell MPK-1 ERK Controls Membrane

    E-Print Network [OSTI]

    Developmental Cell Article MPK-1 ERK Controls Membrane Organization in C. elegans Oogenesis via/ERK in the proximal germ- line to control plasma membrane biogenesis and organization during oogenesis and oocyte production is dramatically downregulated. The RTK-RAS-ERK pathway relays physiological and devel

  18. Modelling the Influence of RKIP on the ERK Signalling Pathway Using the Stochastic Process

    E-Print Network [OSTI]

    Swain, Peter

    Modelling the Influence of RKIP on the ERK Signalling Pathway Using the Stochastic Process Algebra the influence of the Raf Kinase In- hibitor Protein (RKIP) on the Extracellular signal Regulated Kinase (ERK durations and probabilistic choices. The system which we consider is the Ras/Raf-1/MEK/ERK signalling

  19. Tellus (2008), 60A, 3241 C 2007 The Authors Journal compilation C 2007 Blackwell Munksgaard

    E-Print Network [OSTI]

    Allan, Richard P.

    2008-01-01

    , 1 P.P. Shirshov Institute of Oceanology, RAS, 36, Nakhimovsky Ave., Moscow, Russia; 2 Department seasonal differences are found over eastern Europe and European Russia, where the summer PW climatology processes of the climate system. It is the most important greenhouse gas, absorbing strongly a portion

  20. Tellus,Volume 56 Issue 4,page 328341, August 2004 Copyright C Blackwell Munksgaard, 2004

    E-Print Network [OSTI]

    Begstsson, Lennart

    Petersburg, Russia; 8 Obukhov Institute of Atmospheric Physics RAS, Moscow, Russia; 9 Arctic and Antarctic Research Institute, St Petersburg, Russia; 10 Hadley Centre for Climate Prediction and Research, Bracknell to assess the Arctic's response to increasing anthropogenic greenhouse-gas forcing. Here, a new set

  1. Atmos. Chem. Phys., 9, 175187, 2009 www.atmos-chem-phys.net/9/175/2009/

    E-Print Network [OSTI]

    Meskhidze, Nicholas

    Physics RAS, Moscow, Russia Received: 7 May 2008 ­ Published in Atmos. Chem. Phys. Discuss.: 11 August events. The remaining data show an increase in 14CO2 from Western Russia (40 E) to Eastern Siberia (120 E, and gradual dispersion of the fos- sil fuel plume across Northern Asia. Other trace gas species which may

  2. Jiajia Zhou 1, Evgeny S. Asmolov 2,3, Friederike Schmid 1 and Olga I. Vinogradova 2,4 1 Komet331, Institut fr Physik, Johannes Gutenberg-Universitt Mainz, Germany

    E-Print Network [OSTI]

    Zhou, Jiajia

    Chemistry and Electrochemistry, RAS, Russia 3 Central Aero-Hydrodynamic Institute and Institute of Mechanics, Moscow State University, Russia 4 Department of Physics, Moscow State University, Russia and DWI, RWTH on such SH surfaces is observed in the Cassie state, where the textures are filled with gas, which renders

  3. Atmos. Chem. Phys., 9, 41574175, 2009 www.atmos-chem-phys.net/9/4157/2009/

    E-Print Network [OSTI]

    Meskhidze, Nicholas

    of Atmosphere Physics RAS, Moscow, Russia 3International Center for Astronomical, Medical and Ecological- tain Station in Caucasus, Russia (KHMS, 43.70 N, 42.70 E, 2070 m a.s.l.) and the Jungfraujoch radical and it is a significant greenhouse gas (IPCC, 2007). Surface ozone concentration is highly

  4. Decharging of Complex Plasmas: First Kinetic Observations A.V. Ivlev,1

    E-Print Network [OSTI]

    Goree, John

    for High Energy Densities, RAS, 125412 Moscow, Russia 3 Yuri Gagarin Cosmonauts Training Center, 141160 Star City, Moscow Region, Russia 4 RSC ``Energia,'' 141070 Korolev, Russia 5 Department of Physics and predicts the rest charge at lower gas pressures. DOI: 10.1103/PhysRevLett.90.055003 PACS numbers: 52.27.Lw

  5. 2D Mater. 2 (2015) 025002 doi:10.1088/2053-1583/2/2/025002 Graphene vertical cascade interband terahertz and infrared

    E-Print Network [OSTI]

    Mitin, Vladimir

    2015-01-01

    2D Mater. 2 (2015) 025002 doi:10.1088/2053-1583/2/2/025002 PAPER Graphene vertical cascade Technical University and Institute of Ultra High Frequency Semiconductor Electronics of RAS, Moscow 105005, Troy, NY 12180, USA E-mail: v-ryzhii@riec.tohoku.ac.jp Keywords: graphene, radiative transition

  6. arXiv:astro-ph/0607601v228Jul2006 Mon. Not. R. Astron. Soc. 000, 000000 (0000) Printed 31 July 2006 (MN LATEX style file v2.2)

    E-Print Network [OSTI]

    Gomboc, Andreja

    for publication in MNRAS ABSTRACT We present a multiwavelength study of GRB 060108 - the 100th Gamma Ray Burst galaxy. Key words: gamma-rays:bursts c 0000 RAS #12;2 Oates et al. 1 INTRODUCTION Gamma ray bursts (GRBs the burst, from the optical to the X-ray range, is best fit by a simple power law, F - , with OX = 0

  7. UHS Health Promotion Office, 110 Anderson Tower, 273-5775 The Monthly InSTALLment

    E-Print Network [OSTI]

    Cantlon, Jessica F.

    are open. Is the HPV vaccine available at UHS? Yes, the HPV vaccine (Gardasil) is available at UHS health information to students. RAs post each issue in the bathroom stalls on their hall. We kept the middle of this issue the same as the students see, so you can see a Monthly InSTALLment for yourself

  8. Universal Method for Protein Immobilization on Chemically Functionalized Germanium Investigated by ATR-FTIR Difference

    E-Print Network [OSTI]

    Gerwert, Klaus

    of the "on" and "off" switching of Ras at atomic resolution. Furthermore, the activity of immobilized PS I is a widely used label-free technique that detects mass changes at the sensor surface.8 Label-free methods are becoming increasingly popular as the attached label can interfere with the reaction and the labeling

  9. Reflectance anisotropy of uracil covered Si(0 0 1) surfaces: Ab initio predictions

    E-Print Network [OSTI]

    Schmidt, Wolf Gero

    , Max-Wien-Platz 1, 07743 Jena, Germany Received 15 August 2003; accepted for publication 5 November predict the appearance of new peaks for higher coverages, suggesting the usage of RAS for the analysis, investigated experimentally by STM and high-resolution elec- tron energy-loss spectroscopy [8], leads to what

  10. Journal of Mining Science, Vol. 43, No. 4, 2007 ROCK FAILURE

    E-Print Network [OSTI]

    Alexandrov, Victor

    the supervision of N. G. Dubynin, the new technologies were developed for underground iron ore mining409 Journal of Mining Science, Vol. 43, No. 4, 2007 ROCK FAILURE EFFECT OF BOREHOLE CHARGE for underground blasting fragmentation were developed at the Institute of Mining, SB RAS. It was under

  11. Scale dependence of native and alien species richness in North American floras

    E-Print Network [OSTI]

    Palmer, Michael W.

    Scale dependence of native and alien species richness in North American floras Vliv mítka studia na of native and alien species richness in North American flo- ras. ­ Preslia 78: 427­436. I analyzed data from and alien diversity vary as a function of spatial grain. Moving window multi- ple regression revealed

  12. RELIABILITY, AVAILABILITY, AND SERVICEABILITY FOR PETASCALE HIGH-END COMPUTING AND BEYOND

    SciTech Connect (OSTI)

    Chokchai "Box" Leangsuksun

    2011-05-31

    Our project is a multi-institutional research effort that adopts interplay of RELIABILITY, AVAILABILITY, and SERVICEABILITY (RAS) aspects for solving resilience issues in highend scientific computing in the next generation of supercomputers. results lie in the following tracks: Failure prediction in a large scale HPC; Investigate reliability issues and mitigation techniques including in GPGPU-based HPC system; HPC resilience runtime & tools.

  13. Fabrication of Planar Colloidal Clusters with Template-Assisted Interfacial Assembly

    E-Print Network [OSTI]

    Wirth, Christopher L.; De Volder, Michael; Vermant, Jan

    2015-01-29

    spheres. Adv. Mater. 2010, 22, 3104?24. (30) Duffela, B. Van; Ras, R. H. A.; Schryver, C. De; Schoonheydt, R. A. Langmuir?Blodgett deposition and optical diffraction of twodimensional opal. J. Mater. Chem. 2001, 11, 3333?3336. (31) Tolnai, G.; Csempesz...

  14. Summary of Policy Changes for Senior Research Associates and Research Associates (Limited Term)

    E-Print Network [OSTI]

    Toronto, University of

    to SRA and RAs and Principal Investigators later in the spring. Conflict of Interest: This new policy is based on the Conflict of Interest policy for Professional/Managerial employees. Intellectual Property to clarify eligibility for annual across-the-board and merit increases. The SRA salary range will now have

  15. Journal Title: IEEE transactions on antennas ... - Purdue University

    E-Print Network [OSTI]

    Dec 1, 2008 ... [3] M. A. Gintsburg, “Surface waves on the boundary of a plasma in a magnetic ?eld,” RasProst. ... for which the ratio of the characteristic linear dimen- sion of the obstacle to the ..... New York: Cambridge, 1958. [4] J. B. Keller ...

  16. Acute ethanol intake induces superoxide anion generation and mitogen-activated protein kinase phosphorylation in rat aorta: A role for angiotensin type 1 receptor

    SciTech Connect (OSTI)

    Yogi, Alvaro; Callera, Glaucia E.; Mecawi, André S.; Batalhão, Marcelo E.; Carnio, Evelin C.; Antunes-Rodrigues, José; Queiroz, Regina H.; Touyz, Rhian M.; Tirapelli, Carlos R.

    2012-11-01

    Ethanol intake is associated with increase in blood pressure, through unknown mechanisms. We hypothesized that acute ethanol intake enhances vascular oxidative stress and induces vascular dysfunction through renin–angiotensin system (RAS) activation. Ethanol (1 g/kg; p.o. gavage) effects were assessed within 30 min in male Wistar rats. The transient decrease in blood pressure induced by ethanol was not affected by the previous administration of losartan (10 mg/kg; p.o. gavage), a selective AT{sub 1} receptor antagonist. Acute ethanol intake increased plasma renin activity (PRA), angiotensin converting enzyme (ACE) activity, plasma angiotensin I (ANG I) and angiotensin II (ANG II) levels. Ethanol induced systemic and vascular oxidative stress, evidenced by increased plasma thiobarbituric acid-reacting substances (TBARS) levels, NAD(P)H oxidase?mediated vascular generation of superoxide anion and p47phox translocation (cytosol to membrane). These effects were prevented by losartan. Isolated aortas from ethanol-treated rats displayed increased p38MAPK and SAPK/JNK phosphorylation. Losartan inhibited ethanol-induced increase in the phosphorylation of these kinases. Ethanol intake decreased acetylcholine-induced relaxation and increased phenylephrine-induced contraction in endothelium-intact aortas. Ethanol significantly decreased plasma and aortic nitrate levels. These changes in vascular reactivity and in the end product of endogenous nitric oxide metabolism were not affected by losartan. Our study provides novel evidence that acute ethanol intake stimulates RAS activity and induces vascular oxidative stress and redox-signaling activation through AT{sub 1}-dependent mechanisms. These findings highlight the importance of RAS in acute ethanol-induced oxidative damage. -- Highlights: ? Acute ethanol intake stimulates RAS activity and vascular oxidative stress. ? RAS plays a role in acute ethanol-induced oxidative damage via AT{sub 1} receptor activation. ? Translocation of p47phox and MAPKs phosphorylation are downstream effectors. ? Acute ethanol consumption increases the risk for acute vascular injury.

  17. Waveguide detection of right-angle-scattered light in flow cytometry

    DOE Patents [OSTI]

    Mariella, Jr., Raymond P. (Danville, CA)

    2000-01-01

    A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.

  18. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Lenore Rasmussen, David Schramm, Paul Rasmussen, Kevin Mullaly, Ras Labs, LLC, Intelligent Materials for Prosthetics & Automation, Lewis D. Meixler, Daniel Pearlman and Alice Kirk

    2011-05-23

    Ras Labs produces contractile electroactive polymer (EAP) based materials and actuators that bend, swell, ripple, and contract (new development) with low electric input. In addition, Ras Labs produces EAP materials that quickly contract and expand, repeatedly, by reversing the polarity of the electric input, which can be cycled. This phenomenon was explored using molecular modeling, followed by experimentation. Applied voltage step functions were also investigated. High voltage steps followed by low voltage steps produced a larger contraction followed by a smaller contraction. Actuator control by simply adjusting the electric input is extremely useful for biomimetic applications. Muscles are able to partially contract. If muscles could only completely contract, nobody could hold an egg, for example, without breaking it. A combination of high and low voltage step functions could produce gross motor function and fine manipulation within the same actuator unit. Plasma treated electrodes with various geometries were investigated as a means of providing for more durable actuation.

  19. A review of "Sight and Spirituality in Early Netherlandish Painting." by Bret L. Rothstein, 

    E-Print Network [OSTI]

    Tokumitsu, Miya

    2007-01-01

    SEVENTEENTH-CENTURY NEWS volume includes appendices listing the works included in the Recueil g?n?ral des op?ras, a bibliography, and an index nominum. Bret L. Rothstein. Sight and Spirituality in Early Netherlandish Painting. Cambridge: Cambridge University..., Sight and Spirituality in Early Netherlandish Painting, examines four seminal paintings of the fifteenth-century Low Countries and discusses the conse- quences of representing aspects of Christian spirituality for both artist and viewer. Painterly...

  20. 3D spectroscopy of the ionized gas kinematics in galactic rings

    E-Print Network [OSTI]

    Moiseev, A V

    2008-01-01

    The kinematics of galactic rings were studied with a scanning Fabry-Perot interferometer mounted in the multi-mode focal reducer SCORPIO (Afanasiev & Moiseev 2005) at the SAO RAS 6-m telescope. The analysis of the ionized gas velocity fields allows us to understand the nature of the ring formation in several galaxies. The different types of the rings in the presented objects (resonanced, collisional, polar) were caused by the various sorts of interactions: merging, head-on collisions.

  1. 3D spectroscopy of the ionized gas kinematics in galactic rings

    E-Print Network [OSTI]

    A. V. Moiseev

    2008-01-10

    The kinematics of galactic rings were studied with a scanning Fabry-Perot interferometer mounted in the multi-mode focal reducer SCORPIO (Afanasiev & Moiseev 2005) at the SAO RAS 6-m telescope. The analysis of the ionized gas velocity fields allows us to understand the nature of the ring formation in several galaxies. The different types of the rings in the presented objects (resonanced, collisional, polar) were caused by the various sorts of interactions: merging, head-on collisions.

  2. RAF protein-serine/threonine kinases: Structure and regulation

    SciTech Connect (OSTI)

    Roskoski, Robert, E-mail: rrj@brimr.org [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)] [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)

    2010-08-27

    Research highlights: {yields} The formation of unique side-to-side RAF dimers is required for full kinase activity. {yields} RAF kinase inhibitors block MEK activation in cells containing oncogenic B-RAF. {yields} RAF kinase inhibitors can lead to the paradoxical increase in RAF kinase activity. -- Abstract: A-RAF, B-RAF, and C-RAF are a family of three protein-serine/threonine kinases that participate in the RAS-RAF-MEK-ERK signal transduction cascade. This cascade participates in the regulation of a large variety of processes including apoptosis, cell cycle progression, differentiation, proliferation, and transformation to the cancerous state. RAS mutations occur in 15-30% of all human cancers, and B-RAF mutations occur in 30-60% of melanomas, 30-50% of thyroid cancers, and 5-20% of colorectal cancers. Activation of the RAF kinases requires their interaction with RAS-GTP along with dephosphorylation and also phosphorylation by SRC family protein-tyrosine kinases and other protein-serine/threonine kinases. The formation of unique side-to-side RAF dimers is required for full kinase activity. RAF kinase inhibitors are effective in blocking MEK1/2 and ERK1/2 activation in cells containing the oncogenic B-RAF Val600Glu activating mutation. RAF kinase inhibitors lead to the paradoxical increase in RAF kinase activity in cells containing wild-type B-RAF and wild-type or activated mutant RAS. C-RAF plays a key role in this paradoxical increase in downstream MEK-ERK activation.

  3. Intracellular lysyl oxidase: Effect of a specific inhibitor on nuclear mass in proliferating cells

    SciTech Connect (OSTI)

    Saad, Fawzy A. [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States) [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States); Torres, Marie [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States)] [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States); Wang, Hao [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States) [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States); Graham, Lila, E-mail: lilagraham@cs.com [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States) [Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedics, Children's Hospital Boston, 300 Longwood Avenue EN926, Boston, MA 02115 (United States); Harvard Medical School, Boston, MA 02115 (United States)

    2010-06-11

    LOX, the principal enzyme involved in crosslinking of collagen, was the first of several lysyl oxidase isotypes to be characterized. Its active form was believed to be exclusively extracellular. Active LOX was later reported to be present in cell nuclei; its function there is unknown. LOX expression opposes the effect of mutationally activated Ras, which is present in about 30% of human cancers. The mechanism of LOX in countering the action of Ras is also unknown. In the present work, assessment of nuclear protein for possible effects of lysyl oxidase activity led to the discovery that proliferating cells dramatically increase their nuclear protein content when exposed to BAPN ({beta}-aminopropionitrile), a highly specific lysyl oxidase inhibitor that reportedly blocks LOX inhibition of Ras-induced oocyte maturation. In three cell types (PC12 cells, A7r5 smooth muscle cells, and NIH 3T3 fibroblasts), BAPN caused a 1.8-, 1.7-, and 2.1-fold increase in total nuclear protein per cell, respectively, affecting all major components in both nuclear matrix and chromatin fractions. Since nuclear size is correlated with proliferative status, enzyme activity restricting nuclear growth may be involved in the lysyl oxidase tumor suppressive effect. Evidence is also presented for the presence of apparent lysyl oxidase isotype(s) containing a highly conserved LOX active site sequence in the nuclei of PC12 cells, which do not manufacture extracellular lysyl oxidase substrates. Results reported here support the hypothesis that nuclear lysyl oxidase regulates nuclear growth, and thereby modulates cell proliferation.

  4. A high frequency resonance gravity gradiometer

    SciTech Connect (OSTI)

    Bagaev, S. N.; Kvashnin, N. L.; Skvortsov, M. N.; Bezrukov, L. B.; Krysanov, V. A.; Oreshkin, S. I.; Motylev, A. M.; Popov, S. M.; Samoilenko, A. A.; Yudin, I. S.; Rudenko, V. N.

    2014-06-15

    A new setup OGRAN—the large scale opto-acoustical gravitational detector is described. As distinguished from known gravitational bar detectors it uses the optical interferometrical readout for registering weak variations of gravity gradient at the kilohetz frequency region. At room temperature, its sensitivity is limited only by the bar Brownian noise at the bandwidth close to 100 Hz. It is destined for a search for rare events—gravitational pulses coincident with signals of neutrino scintillator (BUST) in the deep underground of Baksan Neutrino Observatory of INR RAS.

  5. Merging as a key to reforming of disk and AGN triggering in Sy galaxy Mrk334

    E-Print Network [OSTI]

    A. A. Smirnova; A. V. Moiseev

    2008-01-10

    We have studied the kinematics of the ionized gas and stellar component in Mrk334 using methods of panoramic (3D) spectroscopy. The observations were performed at the prime focus of the SAO RAS 6-m telescope with the integral-field spectrograph MPFS (Afanasiev et al. 2001) and with a scanning Fabry-Perot interferometer (FPI), installed on the multimode device SCORPIO (Afanasiev & Moiseev 2005). Based on these data, the monochromatic maps and velocity fields in different emission lines of the ionized gas were constructed. The diagnostic diagrams have been made based on the emission lines ratios.

  6. Merging as a key to reforming of disk and AGN triggering in Sy galaxy Mrk334

    E-Print Network [OSTI]

    Smirnova, A A

    2008-01-01

    We have studied the kinematics of the ionized gas and stellar component in Mrk334 using methods of panoramic (3D) spectroscopy. The observations were performed at the prime focus of the SAO RAS 6-m telescope with the integral-field spectrograph MPFS (Afanasiev et al. 2001) and with a scanning Fabry-Perot interferometer (FPI), installed on the multimode device SCORPIO (Afanasiev & Moiseev 2005). Based on these data, the monochromatic maps and velocity fields in different emission lines of the ionized gas were constructed. The diagnostic diagrams have been made based on the emission lines ratios.

  7. Biology of childhood germ cell tumours, focussing on the significance of microRNAs

    E-Print Network [OSTI]

    Murray, Matthew; Nicholson, James; Coleman, Nicholas

    2014-10-09

    negatively regulated by the RNA binding protein LIN28 (Viswanathan et al., 2008, 2009), which is expressed at high levels in PGCs (West et al., 2009). Early studies used immunohistochemistry (Cao et al., 2011a,b; Xue et al., 2011) and RNA interference (Gillis... – microRNAs with a role in cancer. Nat Rev Cancer 6, 259–269. Feldman DR, Iyer G, Van Alstine L, Patil S, Al-Ahmadie HA, Reuter VE, Bosl GJ, Chaganti RS & Solit DB. (2014) Presence of somatic mutations within PIK3CA, AKT, RAS, and FGFR3 but not BRAF...

  8. The use of oxidation-reduction dyes in the determination of the shelf-life of meats 

    E-Print Network [OSTI]

    Bush, Janis Carolyn

    1970-01-01

    and placed in 50 ml Bacto- rept&?! . . This suspension !ras t!! e used beth fnr the nus&erical estr- n, &ti on aud for &. hv dyc. redo? i?n& test . . . A 9 ml p&sction nf bc th thc m a' emulsion and the soaper!srnn from th- p!Dvr was pipe t ted in& o s..., 1970) Janis C. Bush, B. S. (Biology), Abilene Christian College Directed by: Dr. Carl Vanderzant A technique w's developed to carry out dye reduction tests with a m'nimum ot interference of tissu- in the reduction process. Tne technique e...

  9. Cotton Production in Texas. 

    E-Print Network [OSTI]

    Reynolds, E. B.

    1959-01-01

    ........ Type 2 . Storm-resistant Big Boll 8 ............ Type 3 . Western Open Boll 8 ............ Type 4 . Texas Stormproof 8 .......... Type 5 . Trans-Pecos Irrigated 8 ...... . Type 6 Medium-staple Open Boll 8 Type 7 . American-Egyptian ............ 9... was named Acala. 'Today this variety is widely grown in the Trans-Pecos area of Texas and in New 2 first commercial production of cotton in R/T~~~~~, ~~i~~~~ and california. ras made by the Stephen F. Austin colonists, 1\\110 mtled along the lower Brazos...

  10. Discovery of a Novel Compound with Anti-Venezuelan Equine Encephalitis Virus Activity That Targets the Nonstructural Protein 2

    E-Print Network [OSTI]

    Chung, Dong-Hoon; Jonsson, Colleen B.; Tower, Nichole A.; Chu, Yong-Kyu; Sahin, Ergin; Golden, Jennifer E.; Noah, James W.; Schroeder, Chad E.; Stosky, Julie B.; Sosa, Melinda; Cramer, Daniel E.; McKellip, Sara N.; Rasmussen, Lynn; White, E. Lucile; Schmaljohn, Connie S.; Julander, Justin G.; Smith, Jeffery M.; Filone, Claire Marie; Connor, John H.; Sakurai, Yasuteru; Davey, Robert A.

    2014-06-26

    assays. Detailed procedures for these procedures are described in elsewhere (PubChem AID: 588727. http://pubchem.ncbi.nlm. nih.gov/assay/assay.cgi?aid = 588727&loc = ea_ras). A similar approach was used to measure the dose-response inhibition... reagents and World Reference Center for Emerging Viruses and Arboviruses for VEEV TrD. Author Contributions Conceived and designed the experiments: DHC CBJ ELW JEG CSS JGJ JHC RAD. Performed the experiments: DHC NAT YKC ES JWN CES JBS DEC SNM LR JGJ JMS CMF...

  11. Identification of Scaffold Proteins RACK1 and Hic-5 as Regulators of Endothelial Sprouting Resoponses 

    E-Print Network [OSTI]

    Dave, Jui Mayank

    2014-12-12

    this dynamic process, since standard two dimensional (2D) cell culture techniques fail to replicate individual steps of angiogenesis such as sprout initiation, invasion, and lumen formation. Tumor angiogenic studies gained momentum when Judah Folkman first... Cγ (PLCγ) pathway, phosphoinositol 3-kinase (PI3K) and Ras/MEK/MAPK pathway [88, 89]. Basic fibroblast growth factor (bFGF) or FGF2 is a heparin binding growth factor, which was first discovered by Judah Folkman in 1984 [90]. This 18.8 k...

  12. Compositions and methods for detecting Noonan syndrome

    DOE Patents [OSTI]

    Gelb, Bruce D. (New York, NY); Tartaglia, Marco (Rome, IT); Pennacchio, Len (Walnut Creek, CA)

    2012-07-17

    Diagnostic and therapeutic applications for Noonan Syndrome are described. The diagnostic and therapeutic applications are based on certain mutations in a RAS-specific guanine nucleotide exchange factor gene SOS1 or its expression product. The diagnostic and therapeutic applications are also based on certain mutations in a serine/threonine protein kinase gene RAF1 or its expression product thereof. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to RAF1 or SOS1, and variants thereof, as well as host cells expressing such variants.

  13. Intervention in Countries with Unsustainable Energy Policies: Is it Ever Justifiable?

    SciTech Connect (OSTI)

    Tonn, Bruce Edward

    2010-08-01

    This paper explores whether it is ever justifiable for the international community to forcibly intervene in countries that have unsustainable energy policies. The literature on obligations to future generations suggests, philosophically, that intervention might be justified under certain circumstances. Additionally, the world community has intervened in the affairs of other countries for humanitarian reasons, such as in Kosovo, Somalia, and Haiti. However, intervention to deal with serious energy problems is a qualitatively different and more difficult problem. A simple risk analysis framework is used to organize the discussion about possible conditions for justifiable intervention. If the probability of deaths resulting from unsustainable energy policies is very large, if the energy problem can be attributed to a relatively small number of countries, and if the risk of intervention is acceptable (i.e., the number of deaths due to intervention is relatively small), then intervention may be justifiable. Without further analysis and successful solution of several vexing theoretical questions, it cannot be stated whether unsustainable energy policies being pursued by countries at the beginning of the 21st century meet the criteria for forcible intervention by the international community.

  14. Prospects for coal briquettes as a substitute fuel for wood and charcoal in US Agency for International Development Assisted countries

    SciTech Connect (OSTI)

    Perlack, R.D.; Stevenson, G.G.; Shelton, R.B.

    1986-02-01

    Fuelwood shortages and potential shortages are widespread throughout the developing world, and are becoming increasingly more prevalent because of the clearing of land for subsistence and plantation agriculture, excessive and inefficient commercial timber harvesting for domestic and export construction, and charcoal production to meet rising urban demands. Further, the environmental and socioeconomic consequences of the resulting deforestation are both pervasive and complex. This report focuses on the substitution of coal briquettes for fuelwood. Although substantial adverse health effects could be expected from burning non-anthracite coal or coal briquettes, a well-developed technique, carbonization, exists to convert coal to a safer form for combustion. The costs associated with briquetting and carbonizing coal indicate that ''smokeless'' coal briquettes can be produced at costs competitive with fuelwood and charcoal. The US Agency for International Development (USAID) is working on implementing this energy option in Haiti and Pakistan by (1) evaluating resources, (2) assessing markets, (3) analyzing technologies, (4) studying government policy and planning, and (5) packaging the idea for the private sector to implement. 26 refs., 2 figs., 12 tabs.

  15. Annotated bibliography of coal in the Caribbean region. [Lignite

    SciTech Connect (OSTI)

    Orndorff, R.C.

    1985-01-01

    The purpose of preparing this annotated bibliography was to compile information on coal localities for the Caribbean region used for preparation of a coal map of the region. Also, it serves as a brief reference list of publications for future coal studies in the Caribbean region. It is in no way an exhaustive study or complete listing of coal literature for the Caribbean. All the material was gathered from published literature with the exception of information from Cuba which was supplied from a study by Gordon Wood of the US Geological Survey, Branch of Coal Resources. Following the classification system of the US Geological Survey (Wood and others, 1983), the term coal resources has been used in this report for reference to general estimates of coal quantities even though authors of the material being annotated may have used the term coal reserves in a similar denotation. The literature ranges from 1857 to 1981. The countries listed include Colombia, Mexico, Venezuela, Cuba, the Dominican Republic, Haiti, Jamaica, Puerto Rico, and the countries of Central America.

  16. Remedial action plan and site design for stabilization of the inactive uranium processing site at Naturita, Colorado. Remedial Action Selection Report, Appendix B of Attachment 2: Geology report, Final

    SciTech Connect (OSTI)

    Not Available

    1994-03-01

    The uranium processing site near Naturita, Colorado, is one of 24 inactive uranium mill sites designated to be cleaned up by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA), 42 USC {section} 7901 et seq. Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which describes the proposed remedial action for the Naturita site. An extensive amount of data and supporting information has been generated and evaluated for this remedial action. These data and supporting information are not incorporated into this single document but are included or referenced in the supporting documents. The RAP consists of this RAS and four supporting documents or attachments. This Attachment 2, Geology Report describes the details of geologic, geomorphic, and seismic conditions at the Dry Flats disposal site.

  17. Evolution of gas processing industry in Saudi Arabia

    SciTech Connect (OSTI)

    Showail, A.

    1983-01-01

    The beginning of the natural gas processing industry in Saudi Arabia is traced back to 1959 when Aramco embarked on a program to recover natural gas liquids (NGL) for export from low pressure gases such as stabilizer overhead, spheroid, tank farm, and refinery off-gases. The processing scheme involves compression and refrigeration to extract C3+ raw NGL, a raw NGL gathering system, and a fractionation plant to separate propane, butane, and natural gasoline. NGL extracted in Abqaiq and Ras Tanura is moved to Ras Tanura for fractionation, storage, and export. The system, built in several increments, has total design capacity of 500 MMscfd of feed gases to produce 320,000 bpd of NGL composed of 40% propane, 30% butane, and 30% natural gasoline. Phase II of the Saudi gas program envisages collection and processing of associated gas produced with Arabian medium and heavy crude oils largely in the northern onshore and offshore fields. Further domestic development may focus on more diversification in gas product utilization and on upgrading to higher value products.

  18. MEK1/2 dual-specificity protein kinases: Structure and regulation

    SciTech Connect (OSTI)

    Roskoski, Robert, E-mail: rrj@brimr.org [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)] [Blue Ridge Institute for Medical Research, 3754 Brevard Road, Suite 116, Box 19, Horse Shoe, NC 28742 (United States)

    2012-01-06

    Highlights: Black-Right-Pointing-Pointer MEK1 is activated by phosphorylation of S218 and S222 in its activation segment. Black-Right-Pointing-Pointer MEK1 activation requires KSR, which functions as a scaffold and a protein kinase. Black-Right-Pointing-Pointer S212 phosphorylation in the MEK1 activation segment is inhibitory. Black-Right-Pointing-Pointer MEK1 and MEK2 contain a catalytic and a regulatory spine. -- Abstract: MEK1 and MEK2 are related protein kinases that participate in the RAS-RAF-MEK-ERK signal transduction cascade. This cascade participates in the regulation of a large variety of processes including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Moreover, oncogenic mutations in RAS or B-RAF are responsible for a large proportion of human cancers. MEK1 is activated by phosphorylation of S218 and S222 in its activation segment as catalyzed by RAF kinases in an intricate process that involves a KSR scaffold. Besides functioning as a scaffold, the kinase activity of KSR is also required for MEK activation. MEK1 regulation is unusual in that S212 phosphorylation in its activation segment is inhibitory. Moreover, active ERK catalyzes a feedback inhibitory phosphorylation of MEK1 T292 that serves to downregulate the pathway.

  19. Prorenin induces ERK activation in endothelial cells to enhance neovascularization independently of the renin-angiotensin system

    SciTech Connect (OSTI)

    Uraoka, Maki [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)] [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan); Ikeda, Koji, E-mail: ikedak@koto.kpu-m.ac.jp [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)] [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan); Nakagawa, Yusuke; Koide, Masahiro; Akakabe, Yoshiki; Nakano-Kurimoto, Ritsuko; Takahashi, Tomosaburo; Matoba, Satoaki; Yamada, Hiroyuki; Okigaki, Mitsuhiko; Matsubara, Hiroaki [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)] [Department of Cardiovascular Medicine, Kyoto Prefectural University School of Medicine, 465 Kajii, Kawaramachi-Hirokoji, Kamigyo, Kyoto 602-8566 (Japan)

    2009-12-25

    Prorenin is an enzymatically inactive precursor of renin, and its biological function in endothelial cells (ECs) is unknown despite its relevance with the incidence of diabetic microvascular complications. Recently, (pro)renin receptor was identified, and the receptor-associated prorenin system has been discovered, whereas its expression as well as function in ECs remain unclear. In the present study, we found that ECs express the (pro)renin receptor, and that prorenin provoked ERK activation through (pro)renin receptor independently of the renin-angiotensin system (RAS). Prorenin stimulated the proliferation, migration and tube-formation of ECs, while it inhibited endothelial apoptosis induced by serum and growth factor depletion. MEK inhibitor abrogated these proangiogenic effects of prorenin, while AT1 receptor antagonist or angiotensin-converting enzyme inhibitor failed to block them. In vivo neovascularization in the Matrigel-plugs implanted into mouse flanks was significantly enhanced by prorenin, in which significant ERK activation was detected in ECs. Furthermore, tumor xenografts stably transfected with prorenin demonstrated the significantly accelerated growth rate concomitantly with enhanced intratumoral neovascularization. Our data demonstrated that the RAS-independent (pro)renin receptor-mediated signal transduction plays a pivotal role in the regulation of ECs function as well as in the neovascularization, and thus prorenin is potentially involved in the pathophysiology of diabetic microvascular complications as well as cancers.

  20. Cellular oncogene expression following exposure of mice to {gamma}-rays

    SciTech Connect (OSTI)

    Anderson, A.; Woloschak, G.E.

    1991-06-12

    We examined the effects of total body exposure of BCF1 mice to {gamma}-rays (300 cGy) in modulating expression of cellular oncogenes in both gut and liver tissues. We selected specific cellular oncogenes (c-fos, c-myc, c-src, and c-H-ras), based on their normal expression in liver and gut tissues from untreated mice. As early as 5 min. following whole body exposure of BCF1 mice to {gamma}-rays we detected induction of mRNA specific for c-src and c-H-ras in both liver and gut tissues. c-fos RNA was slightly decreased in accumulation in gut but was unaffected in liver tissue from irradiated mice relative to untreated controls. c-myc mRNA accumulation was unaffected in all tissues examined. These experiments document that modulation of cellular oncogene expression can occur as an early event in tissues following irradiation and suggest that this modulation may play a role in radiation-induced carcinogenesis.

  1. Geranylgeranylacetone inhibits ovarian cancer progression in vitro and in vivo

    SciTech Connect (OSTI)

    Hashimoto, Kae; Morishige, Ken-ichirou . E-mail: mken@gyne.med.osaka-u.ac.jp; Sawada, Kenjiro; Ogata, Seiji; Tahara, Masahiro; Shimizu, Shoko; Sakata, Masahiro; Tasaka, Keiichi; Kimura, Tadashi

    2007-04-27

    Geranylgeranylacetone (GGA), an isoprenoid compound, is an anti-ulcer drug developed in Japan. In our previous study, GGA was shown to inhibit ovarian cancer invasion by attenuating Rho activation [K. Hashimoto, K. Morishige, K. Sawada, M. Tahara, S. Shimizu, M. Sakata, K. Tasaka, Y. Murata, Geranylgeranylacetone inhibits lysophosphatidic acid-induced invasion of human ovarian carcinoma cells in vitro. Cancer 103 (2005) 1529-1536.]. In the present study, GGA treatment inhibited ovarian cancer progression in vitro and suppressed the tumor growth and ascites in the in vivo ovarian cancer model. In vitro analysis, treatment of cancer cells by GGA resulted in the inhibition of cancer cell proliferation, the inactivation of Ras, and the suppression of tyrosine phosphorylation of mitogen-activated protein kinase (MAPK). In conclusion, this is the first report that GGA inhibited ovarian cancer progression and the anti-tumor effect by GGA is, at least in part, derived not only from the suppression of Rho activation but also Ras-MAPK activation.

  2. The Adipose Renin-Angiotensin System Modulates Systemic Markers of Insulin Sensitivity and Activates the Intrarenal Renin-Angiotensin System

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kim, Suyeon; Soltani-Bejnood, Morvarid; Quignard-Boulange, Annie; Massiera, Florence; Teboul, Michele; Ailhaud, Gerard; Kim, Jung Han; Moustaid-Moussa, Naima; Voy, Brynn H.

    2006-01-01

    Background. The adipose tissue renin-angiotensin system (RAS) contributes to regulation of fat mass and may also impact systemic functions such as blood pressure and metabolism.Methods and results. A panel of mouse models including mice lacking angiotensinogen,Agt(Agt-KO), mice expressingAgtsolely in adipose tissue (aP2-Agt/Agt-KO), and mice overexpressingAgtin adipose tissue (aP2-Agt) was studied. Total body weight, epididymal fat pad weight, and circulating levels of leptin, insulin, and resistin were significantly decreased inAgt-KO mice, while plasma adiponectin levels were increased. aP2-Agtmice exhibited increased adiposity and plasma leptin and insulin levels compared to wild type (WT) controls. Angiotensinogen and type I Ang II receptor proteinmore »levels were also elevated in kidney of aP2-Agtmice.Conclusion. These findings demonstrate that alterations in adipose RAS activity significantly impact both local and systemic physiology in a way that may contribute to the detrimental health effects of obesity.« less

  3. Soil Management Plan for the Oak Ridge Y-12 National Security Complex Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    2005-03-02

    This Soil Management Plan applies to all activities conducted under the auspices of the National Nuclear Security Administration (NNSA) Oak Ridge Y-12 National Security Complex (Y-12) that involve soil disturbance and potential management of waste soil. The plan was prepared under the direction of the Y-12 Environmental Compliance Department of the Environment, Safety, and Health Division. Soil disturbances related to maintenance activities, utility and building construction projects, or demolition projects fall within the purview of the plan. This Soil Management Plan represents an integrated, visually oriented, planning and information resource tool for decision making involving excavation or disturbance of soil at Y-12. This Soil Management Plan addresses three primary elements. (1) Regulatory and programmatic requirements for management of soil based on the location of a soil disturbance project and/or the regulatory classification of any contaminants that may be present (Chap. 2). Five general regulatory or programmatic classifications of soil are recognized to be potentially present at Y-12; soil may fall under one or more these classifications: (a) Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) pursuant to the Oak Ridge Reservation (ORR) Federal Facilities Agreement; (b) Resource Conservation and Recovery Act (RCRA); (c) RCRA 3004(u) solid waste managements units pursuant to the RCRA Hazardous and Solid Waste Amendments Act of 1984 permit for the ORR; (d) Toxic Substances and Control Act-regulated soil containing polychlorinated biphenyls; and (e) Radiologically contaminated soil regulated under the Atomic Energy Act review process. (2) Information for project planners on current and future planned remedial actions (RAs), as prescribed by CERCLA decision documents (including the scope of the actions and remedial goals), land use controls implemented to support or maintain RAs, RCRA post-closure regulatory requirements for former waste management units, legacy contamination source areas and distribution of contamination in soils, and environmental infrastructure (e.g., caps, monitoring systems, etc.) that is in place or planned in association with RAs. (3) Regulatory considerations and processes for management and disposition of waste soil upon generation, including regulatory drivers, best management practices (BMPs), waste determination protocols, waste acceptance criteria, and existing waste management procedures and BMPs for Y-12. This Soil Management Plan provides information to project planners to better coordinate their activities with other organizations and programs with a vested interest in soil disturbance activities at Y-12. The information allows project managers and maintenance personnel to evaluate and anticipate potential contaminant levels that may be present at a proposed soil disturbance site prior to commencement of activities and allows a more accurate assessment of potential waste management requirements.

  4. Scanning the landscape of genome architecture of non-O1 and non-O139 Vibrio cholerae by whole genome mapping reveals extensive population genetic diversity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chapman, Carol; Henry, Matthew; Bishop-Lilly, Kimberly A.; Awosika, Joy; Briska, Adam; Ptashkin, Ryan N.; Wagner, Trevor; Rajanna, Chythanya; Tsang, Hsinyi; Johnson, Shannon L.; et al

    2015-03-20

    Historically, cholera outbreaks have been linked to V. cholerae O1 serogroup strains or its derivatives of the O37 and O139 serogroups. A genomic study on the 2010 Haiti cholera outbreak strains highlighted the putative role of non O1/non-O139 V. cholerae in causing cholera and the lack of genomic sequences of such strains from around the world. Here we address these gaps by scanning a global collection of V. cholerae strains as a first step towards understanding the population genetic diversity and epidemic potential of non O1/non-O139 strains. Whole Genome Mapping (Optical Mapping) based bar coding produces a high resolution, orderedmore »restriction map, depicting a complete view of the unique chromosomal architecture of an organism. To assess the genomic diversity of non-O1/non-O139 V. cholerae, we applied a Whole Genome Mapping strategy on a well-defined and geographically and temporally diverse strain collection, the Sakazaki serogroup type strains. Whole Genome Map data on 91 of the 206 serogroup type strains support the hypothesis that V. cholerae has an unprecedented genetic and genomic structural diversity. Interestingly, we discovered chromosomal fusions in two unusual strains that possess a single chromosome instead of the two chromosomes usually found in V. cholerae. We also found pervasive chromosomal rearrangements such as duplications and indels in many strains. The majority of Vibrio genome sequences currently in public databases are unfinished draft sequences. The Whole Genome Mapping approach presented here enables rapid screening of large strain collections to capture genomic complexities that would not have been otherwise revealed by unfinished draft genome sequencing and thus aids in assembling and finishing draft sequences of complex genomes. Furthermore, Whole Genome Mapping allows for prediction of novel V. cholerae non-O1/non-O139 strains that may have the potential to cause future cholera outbreaks.« less

  5. General and Versatile Autoinhibition of PLC Isozymes

    SciTech Connect (OSTI)

    Hicks, Stephanie N.; Jezyk, Mark R.; Gershburg, Svetlana; Seifert, Jason P.; Harden, T. Kendall; Sondek, John (UNC)

    2008-10-31

    Phospholipase C (PLC) isozymes are directly activated by heterotrimeric G proteins and Ras-like GTPases to hydrolyze phosphatidylinositol 4,5-bisphosphate into the second messengers diacylglycerol and inositol 1,4,5-trisphosphate. Although PLCs play central roles in myriad signaling cascades, the molecular details of their activation remain poorly understood. As described here, the crystal structure of PLC-{beta}2 illustrates occlusion of the active site by a loop separating the two halves of the catalytic TIM barrel. Removal of this insertion constitutively activates PLC-{beta}2 without ablating its capacity to be further stimulated by classical G protein modulators. Similar regulation occurs in other PLC members, and a general mechanism of interfacial activation at membranes is presented that provides a unifying framework for PLC activation by diverse stimuli.

  6. Nested methylation-specific polymerase chain reaction cancer detection method

    DOE Patents [OSTI]

    Belinsky, Steven A. (Albuquerque, NM); Palmisano, William A. (Edgewood, NM)

    2007-05-08

    A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.

  7. Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins

    SciTech Connect (OSTI)

    Deng, Lin; Department of Oncology, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710038 ; Lu, Yuanyuan; Zhao, Xiaodi; Sun, Yi; Shi, Yongquan; Fan, Hongwei; Liu, Changhao; Zhou, Jinfeng; Nie, Yongzhan; Wu, Kaichun; Fan, Daiming; Guo, Xuegang

    2013-10-18

    Highlights: •Overexpression of Ran in pancreatic cancer was correlated with histological grade. •Downregulation of Ran could induce cell apoptosis and inhibit cell proliferation. •The effects were mediated by cell cycle proteins, Survivin and cleaved Caspase-3. -- Abstract: Ran, a member of the Ras GTPase family, has important roles in nucleocytoplasmic transport. Herein, we detected Ran expression in pancreatic cancer and explored its potential role on tumour progression. Overexpressed Ran in pancreatic cancer tissues was found highly correlated with the histological grade. Downregulation of Ran led to significant suppression of cell proliferation, cell cycle arrest at the G1/S phase and induction of apoptosis. In vivo studies also validated that result. Further studies revealed that those effects were at least partly mediated by the downregulation of Cyclin A, Cyclin D1, Cyclin E, CDK2, CDK4, phospho-Rb and Survivin proteins and up regulation of cleaved Caspase-3.

  8. Fiscal Year 2007 Phased Construction Completion Report for the Zone 2 Soils, Slabs, and Subsurface Structures at East Tennessee Technology Park, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    RSI

    2008-03-01

    The purpose of this Phased Construction Completion Report (PCCR) is to present the fiscal year (FY) 2007 results of characterization activities and recommended remedial actions (RAs) for 11 exposure units (EUs) in Zone 2 (Z2-01, Z2-03, Z2-08, Z2-23, Z2-24, Z2-28, Z2-34, Z2-37, Z2-41, Z2-43, and Z2-44) at the East Tennessee Technology Park (ETTP), which is located in the northwest corner of the U.S. Department of Energy (DOE) Oak Ridge Reservation in Oak Ridge, Tennessee (Fig. 1). ETTP encompasses a total land area of approximately 5000 acres that has been subdivided into three zones--Zone 1 ({approx}1400 acres), Zone 2 ({approx}800 acres), and the Boundary Area ({approx}2800 acres). Zone 2, which encompasses the highly industrialized portion of ETTP shown in Fig. 1, consists of all formerly secured areas of the facility, including the large processing buildings and direct support facilities; experimental laboratories and chemical and materials handling facilities; materials storage and waste disposal facilities; secure document records libraries; and shipping and receiving warehouses. The Zone 2 Record of Decision for Soil, Buried Waste, and Subsurface Structure Actions in Zone 2, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE 2005) (Zone 2 ROD) specifies the future end use for Zone 2 acreage as uncontrolled industrial for the upper 10 ft of soils. Characterization activities in these areas were conducted in compliance with the Zone 2 ROD and the Dynamic Verification Strategy (DVS) and data quality objectives (DQOs) presented in the Remedial Design Report/Remedial Action Work Plan for Zone 2 Soils, Slabs, and Subsurface Structures, East Tennessee Technology Park, Oak Ridge, Tennessee (DOE 2007) (Zone 2 RDR/RAWP). The purpose of this PCCR is to address the following: (1) Document DVS characterization results for the accessible EUs in FY 2007; (2) Describe and document the risk evaluation for each EU, and determine if the EU met the Zone 2 ROD requirements for unrestricted industrial use to 10 ft bgs; (3) Identify additional areas not defined in the Zone 2 ROD that require remediation based on the DVS evaluation results; and (4) Describe the RAs performed in Zone 2. The Zone 2 ROD divided the Zone 2 area into 7 geographic areas and 44 EUs. To facilitate the DQOs of the DVS process, the Zone 2 RDR/RAWP regrouped the 44 EUs into 12 DQO scoping EU groups. These groups facilitated the DQO process by placing similar facilities and their support facilities together and allowed identification of data gaps. The EU groups were no longer pertinent after DQO planning was completed, and characterization was conducted as areas became accessible. As the opportunity to complete characterization became available, the planned DVS program was executed and completed in FY 2007 for the 11 EUs addressed in this document. The main body of this report describes both the DVS process and scope of work performed and the RAs completed. The scope and approach for performing DVS activities performed in FY 2007 that lead to action/no further action decisions are presented in Sects. 2 through 4. RAs performed in FY 2007 are presented in Sects. 5 through 10. Future land use is described in Sect. 11, and the status of all Zone 2 EUs as of this PCCR is presented in Sect. 12.

  9. Remote sensing of seawater and drifting ice in Svalbard fjords by compact Raman LIDAR

    E-Print Network [OSTI]

    Bunkin, Alexey F; Lednev, Vasily N; Lushnikov, Dmitry L; Marchenko, Aleksey V; Morozov, Eugene G; Pershin, Sergey M; Yulmetov, Renat N

    2013-01-01

    A compact Raman LIDAR system for remote sensing of sea and drifting ice was developed at the Wave Research Center at the Prokhorov General Physics Institute of the RAS. The developed system is based on a diode pumped solid state YVO4:Nd laser combined with compact spectrograph equipped with gated detector. The system exhibits high sensitivity and can be used for mapping or depth profiling of different parameters within many oceanographic problems. Light weight (~20 kg) and low power consumption (300 W) make possible to install the device on any vehicle including unmanned aircraft or submarine system. The Raman LIDAR presented was used for Svalbard fjords study and analysis of different influence of the open sea and glaciers on the water properties. Temperature, phytoplankton, and dissolved organic matter distributions in the seawater were studied in the Ice Fjord, Van Mijen Fjord and Rinders Fjord. Drifting ice and seawater in the Rinders Fjord were characterized by the Raman spectroscopy and fluorescence. It...

  10. CANCELLED EMT and back again: does cellular plasticity fuelneoplasticprogressi on?

    SciTech Connect (OSTI)

    Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, MinaJ.

    2007-02-24

    Epithelial-mesenchymal transition (EMT) is a cellular transdifferentiation program that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. However, a similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, in which it is associated with disease progression. EMT in cancer epithelial cells often appears to be an incomplete and bi-directional process. Here we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the Ras-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  11. Mechanisms of disease: epithelial-mesenchymal transition and back again: does cellular plasticity fuel neoplastic progression?

    SciTech Connect (OSTI)

    Bissell, Mina J; Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, Mina J.

    2008-02-13

    Epithelial-mesenchymal transition (EMT) is a conversion that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. A similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, which is associated with disease progression. EMT in cancer epithelial cells often seems to be an incomplete and bi-directional process. In this Review, we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the RAS-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  12. Astrometrical Observations of Pluto - Charon System with the Automated Telescopes of Pulkovo Observatory

    E-Print Network [OSTI]

    Devyatkin, Alexander V; Slesarenko, Vyacheslav Yu

    2015-01-01

    The space probe 'New Horizon' was launched on 19th of January 2006 in order to study Pluto and its moons. Spacecraft will fly by Pluto as close as 12500 km in the middle of July 2015 and will get the most detailed images of Pluto and its moon until this moment. At the same time, observation obtained by the ground-based telescopes may also be helpful for the research of such distant system. Thereby, the Laboratory of observational astrometry of Pulkovo Observatory of RAS made a decision to reprocess observations obtained during last decade. More than 350 positional observations of Pluto - Charon system were carried out with the mirror astrograph ZA-320M at Pulkovo and Maksutov telescope MTM-500M near Kislovodsk. These observations were processed by means of software system APEX-II developed in Pulkovo observatory and numerical simulation was performed to calculate the differences between positions of photocenter and barycenter of Pluto - Charon system.

  13. Applications of radon distribution and radon flux for the determination of oceanic mixing and air-sea gas exchange 

    E-Print Network [OSTI]

    Brewer, Robert Lewis

    1977-01-01

    radium content of 5. 76 X 10 gm f v it'n a maximum deviation from the mean of 12. 3'yo. The Columbian Basin was found Lo have an average Ra concentration of 5, 34 10 gin f + 17. 6~/i and the Yucatan Basin had an average of -14 4. 89 x 10 1 ? gm. 2 -i... counting cell attached to the third segment of the transfer board was evacuated. TO TRAPS H2 ARD 3 I ~i RiER;T TRAP PRFSSiJRE FRCV RA&S 1, 2 AKD 3 ASCARi TE (~P HOLD VACUJV CCKTA!KER TR, Sc S A", . ? L TRAP V C LI U TO ~AREL IA' TER...

  14. Report on the research conducted under the funding of the Sloan foundation postdoctoral fellowship in Computational Molecular Biology [Systematic study of protein-protein complexes] Final report

    SciTech Connect (OSTI)

    Sheinerman, Felix

    2001-06-01

    A central question in molecular biology is what structural features are common at protein-protein interfaces and what energetic factors define the affinity and specificity of protein-protein association. Analysis of structural and mutational data on protein-protein interfaces revealed that protein-protein interfaces of different functional classes contain many more energetically important charged and polar residues than was previously thought. Since, in the context of protein folding studies, polar interactions are believed to destabilize the folded proteins, this observation raised the question as to the forces that determine the stability of protein complexes. To investigate this issue in detail, the authors developed a number of partitioning schemes that allowed them to investigate the role of selected residues, ion pairs, and networks of polar interactions in protein-protein association. The methods developed were applied to the analysis of four different protein-protein interfaces: the ribonuclease barnase and its inhibitor barstar, the human growth hormone and its receptor, subtype N9 influenze virus neuraminidase and NC41 antibody, and the Ras Binding Domain of kinase cRaf and a Ras homologue Rap1A. The calculations revealed a surprising variability in how polar interactions affect the stability of different complexes. The finding that positions of charged and polar residues on protein-protein interfaces are optimized with respect to electrostatic interactions suggests that this property can be employed for the discrimination between native conformations and trial complexes generated by a docking algorithm. Analysis indicated the presence of SH2 domains in Janus family of non-receptor protein tyrosine kinases.

  15. The BRAF{sup T1799A} mutation confers sensitivity of thyroid cancer cells to the BRAF{sup V600E} inhibitor PLX4032 (RG7204)

    SciTech Connect (OSTI)

    Xing, Joanna [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)] [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States); Liu, Ruixin; Xing, Mingzhao [Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, MD 21287 (United States)] [Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology and Metabolism, The Johns Hopkins University School of Medicine, Baltimore, MD 21287 (United States); Trink, Barry, E-mail: btrink@jhmi.edu [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)] [Division of Head and Neck Cancer Research, Department of Otolaryngology and Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 (United States)

    2011-01-28

    Research highlights: {yields} Exciting therapeutic potential has been recently reported for the BRAF{sup V600E} inhibitor PLX4032 in melanoma. {yields} We tested the effects of PLX4032 on the growth of thyroid cancer cells which often harbor the BRAF{sup V600E} mutation. {yields} We observed a potent BRAF{sup V600E}-dependent inhibition of thyroid cancer cells by PLX4032. {yields} We thus demonstrated an important therapeutic potential of PLX4032 for thyroid cancer. -- Abstract: Aberrant signaling of the Ras-Raf-MEK-ERK (MAP kinase) pathway driven by the mutant kinase BRAF{sup V600E}, as a result of the BRAF{sup T1799A} mutation, plays a fundamental role in thyroid tumorigenesis. This study investigated the therapeutic potential of a BRAF{sup V600E}-selective inhibitor, PLX4032 (RG7204), for thyroid cancer by examining its effects on the MAP kinase signaling and proliferation of 10 thyroid cancer cell lines with wild-type BRAF or BRAF{sup T1799A} mutation. We found that PLX4032 could effectively inhibit the MAP kinase signaling, as reflected by the suppression of ERK phosphorylation, in cells harboring the BRAF{sup T1799A} mutation. PLX4032 also showed a potent and BRAF mutation-selective inhibition of cell proliferation in a concentration-dependent manner. PLX4032 displayed low IC{sub 50} values (0.115-1.156 {mu}M) in BRAF{sup V600E} mutant cells, in contrast with wild-type BRAF cells that showed resistance to the inhibitor with high IC{sub 50} values (56.674-1349.788 {mu}M). Interestingly, cells with Ras mutations were also sensitive to PLX4032, albeit moderately. Thus, this study has confirmed that the BRAF{sup T1799A} mutation confers cancer cells sensitivity to PLX4032 and demonstrated its specific potential as an effective and BRAF{sup T1799A} mutation-selective therapeutic agent for thyroid cancer.

  16. Azathioprine desensitizes liver cancer cells to insulin-like growth factor 1 and causes apoptosis when it is combined with bafilomycin A1

    SciTech Connect (OSTI)

    Hernández-Breijo, Borja [Departamento de Biología de Sistemas, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); Monserrat, Jorge [Departamento de Medicina y Especialidades Médicas, Universidad de Alcalá, 28871 Alcalá de Henares (Spain); Román, Irene D. [Departamento de Biología de Sistemas, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); González-Rodríguez, Águeda [Departamento de Biomedicina y Biotecnología, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); Fernández-Moreno, M. Dolores [Departamento de Biología de Sistemas, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Universidad de Alcalá, 28871 Alcalá de Henares (Spain); and others

    2013-11-01

    Hepatoblastoma is a primary liver cancer that affects children, due to the sensitivity of this tumor to insulin-like growth factor 1 (IGF-1). In this paper we show that azathioprine (AZA) is capable of inhibiting IGF1-mediated signaling cascade in HepG2 cells. The efficiency of AZA on inhibition of proliferation differs in the evaluated cell lines as follows: HepG2 (an experimental model of hepatoblastoma) > Hep3B (derived from a hepatocellular carcinoma) > HuH6 (derived from a hepatoblastoma) ? HuH7 (derived from a hepatocellular carcinoma) = Chang Liver cells (a non-malignant cellular model). The effect of AZA in HepG2 cells has been proven to derive from activation of Ras/ERK/TSC2, leading to activation of mTOR/p70S6K in a sustained manner. p70S6K phosphorylates IRS-1 in serine 307 which leads to the uncoupling between IRS-1 and p85 (the regulatory subunit of PI3K) and therefore causing the lack of response of HepG2 to IGF-1. As a consequence, proliferation induced by IGF-1 is inhibited by AZA and autophagy increases leading to senescence of HepG2 cells. Our results suggest that AZA induces the autophagic process in HepG2 activating senescence, and driving to deceleration of cell cycle but not to apoptosis. However, when simultaneous to AZA treatment the autophagy was inhibited by bafilomycin A1 and the degradation of regulatory proteins of cell cycle (e.g. Rb, E2F, and cyclin D1) provoked apoptosis. In conclusion, AZA induces resistance in hepatoblastoma cells to IGF-1, which leads to autophagy activation, and causes apoptosis when it is combined with bafilomycin A1. We are presenting here a novel mechanism of action of azathioprine, which could be useful in treatment of IGF-1 dependent tumors, especially in its combination with other drugs. - Highlights: • Azathioprine activated Ras/ERK/TSC-2/mTOR/p70S6K signaling pathway in HepG2 cells. • Azathioprine inhibited IGF-1-mediated signaling cascade. • Azathioprine induced autophagy leading to cell cycle arrest. • Cells died by apoptosis when azathioprine was combined with bafilomycin A1.

  17. 2010 Remediation Effectiveness Report for the U.S. Department of Energy Oak Ridge Reservation, Oak Ridge, Tennessee - Data and Evaluations

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2010-09-01

    Under the requirements of the Oak Ridge Reservation (ORR) Federal Facility Agreement (FFA) established between the U.S. Department of Energy (DOE), the U.S. Environmental Protection Agency, (EPA) and the Tennessee Department of Environment and Conservation (TDEC) in 1992, all environmental restoration activities on the ORR are performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Since the 1990s, the environmental restoration activities have experienced a gradual shift from characterization to remediation. As this has occurred, it has been determined that the assessment of the individual and cumulative performance of all ORR CERCLA remedial actions (RAs) is most effectively tracked in a single document. The Remediation Effectiveness Report (RER) is an FFA document intended to collate all ORR CERCLA decision requirements, compare pre- and post-remediation conditions at CERCLA sites, and present the results of any required post-decision remediation effectiveness monitoring. First issued in 1997, the RER has been reissued annually to update the performance histories of completed actions and to add descriptions of new CERCLA actions. Monitoring information used in the 2010 RER to assess remedy performance was collected and/or compiled by DOE's Water Resources Restoration Program (WRRP). Only data used to assess performance of completed actions are provided. In addition to collecting CERCLA performance assessment data, the WRRP also collects baseline data to be used to gauge the effectiveness of future actions once implemented. These baseline data are maintained in the Oak Ridge Environmental Information System and will be reported in future RERs, as necessary, once the respective actions are completed. However, when insufficient data exist to assess the impact of the RAs, e.g., when the RA was only recently completed, a preliminary evaluation is made of early indicators of effectiveness at the watershed scale, such as contaminant trends at surface water integration points (IPs). Long-term stewardship (LTS) information used in this report is collected, compiled, and tracked by the WRRP in conjunction with the Bechtel Jacobs Company LLC (BJC) Surveillance and Maintenance (S&M) program, the BJC Radiation Protection Organization at East Tennessee Technology Park (ETTP), and the ETTP Environmental Compliance Program. Additionally, documentation verifying the implementation of administrative land use controls (LUCs) [i.e., property record restrictions, property record notices, zoning notices, and excavation/penetration permit (EPP) program] is also obtained from many sources throughout the fiscal year (FY), including County Register of Deeds offices for property record restrictions and property record notices, City Planning Commission for zoning notices, and BJC project engineers for EPP program verification. Copies of this documentation are obtained by the WRRP and maintained with the project RER files.

  18. LNG fleet increases in size and capabilities

    SciTech Connect (OSTI)

    Linser, H.J. Jr.; Drudy, M.J.; Endrizzi, F.; Urbanelli, A.A.

    1997-06-02

    The LNG fleet as of early 1997 consisted of 99 vessels with total cargo capacity of 10.7 million cu m, equivalent to approximately 4.5 million tons. One of the newest additions to the fleet, the 137,000-cu m tanker Al Zubarah, is five times the size of the original commercial vessel Methane Princess. Al Zubarah`s first loading of more than 60,000 tons occurred in December 1996 for deliver to Japanese buyers from the newly commissioned Qatargas LNG plant at Ras Laffan. That size cargo contains enough clean-burning energy to heat 60,000 homes in Japan for 1 month. Measuring nearly 1,000 ft long, the tanker is among the largest in the industry fleet and joined 70 other vessels of more than 100,000 cu m. Most LNG tankers built since 1975 have been larger-capacity vessels. The paper discusses LNG shipping requirements, containment systems, vessel design, propulsion, construction, operations and maintenance, and the future for larger vessels.

  19. Management information systems software evaluation

    SciTech Connect (OSTI)

    Al-Tunisi, N.; Ghazzawi, A.; Gruyaert, F.; Clarke, D. [Saudi Aramco, Dhahran (Saudi Arabia). Process and Control Systems Dept.

    1995-11-01

    In November 1993, Saudi Aramco management endorsed a proposal to coordinate the development of the Management Information Systems (MISs) of four concurrent projects for its facilities Controls Modernization Program. The affected projects were the Ras Tanura Refinery Upgrade Project, the Abqaiq Plant Controls Modernization and the Shedgum and Uthmaniyah Gas plants Control Upgrade Projects. All of these projects had a significant requirement of MISs in their scope. Under the leadership of the Process and Control Systems Department, and MIS Coordination Team was formed with representatives of several departments. An MIS Applications Evaluation procedure was developed based on the Kepner Tregoe Decisions Analysis Process and general questionnaires were sent to over a hundred potential Vendors. The applications were divided into several categories, such as: Data Capture and Historization, Human User Interface, Trending, Reporting, Graphic Displays, Data Reconciliation, Statistical Analysis, Expert Systems, Maintenance Applications, Document Management and Operations Planning and Scheduling. For each of the MIS Application areas, detailed follow-up questionnaires were used to short list the candidate products. In May and June 1994, selected Vendors were invited to Saudi Arabia for an Exhibition which was open to all Saudi Aramco employees. In conjunction with this, the Vendors were subjected to a rigorous product testing exercise by independent teams of testers. The paper will describe the methods used and the lessons learned in this extensive software evaluation phase, which was a first for Saudi Aramco.

  20. Remedial action plan and site design for stabilization of the inactive uranium processing site at Naturita, Colorado. Remedial action selection report, Attachment 2, Geology report: Preliminary final

    SciTech Connect (OSTI)

    Not Available

    1993-08-01

    The uranium processing site near Naturita, Colorado, is one of 24 inactive uranium mill sites designated to be cleaned up by the US Department of Energy (DOE) under the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA), Public Law 95-604. Part of the UMTRCA requires that the US Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the US Environmental Protection Agency (EPA). Included in the RAP is this Remedial Action Selection Report (RAS), which serves two purposes. First, it describes the activities that are proposed by the DOE to accomplish remediation and long-term stabilization and control of the radioactive materials at the inactive uranium processing site near Naturita, Colorado. Second, this document and the rest of the RAP, upon concurrence and execution by the DOE, the state of Colorado, and the NRC, become Appendix B of the cooperative agreement between the DOE and the State of Colorado.

  1. Guide to ground water remediation at CERCLA response action and RCRA corrective action sites

    SciTech Connect (OSTI)

    1995-10-01

    This Guide contains the regulatory and policy requirements governing remediation of ground water contaminated with hazardous waste [including radioactive mixed waste (RMW)], hazardous substances, or pollutants/contaminants that present (or may present) an imminent and substantial danger. It was prepared by the Office of Environmental Policy and Assistance, RCRA/CERCLA Division (EH-413), to assist Environmental Program Managers (ERPMs) who often encounter contaminated ground water during the performance of either response actions under CERCLA or corrective actions under Subtitle C of RCRA. The Guide begins with coverage of the regulatory and technical issues that are encountered by ERPM`s after a CERCLA Preliminary Assessment/Site Investigation (PA/SI) or the RCRA Facility Assessment (RFA) have been completed and releases into the environment have been confirmed. It is based on the assumption that ground water contamination is present at the site, operable unit, solid waste management unit, or facility. The Guide`s scope concludes with completion of the final RAs/corrective measures and a determination by the appropriate regulatory agencies that no further response action is necessary.

  2. Remedial action plan and site design for stabilization of the inactive uranium mill tailings site at Falls City, Texas. Remedial action selection report, attachment 2, geology report; attachment 3, groundwater hydrology report; and attachment 4, water resources protection strategy. Final report

    SciTech Connect (OSTI)

    1992-09-01

    The uranium processing site near Falls City, Texas, was one of 24 inactive uranium mill sites designated to be remediated by the U.S. Department of Energy (DOE) under Title I of the Uranium Mill Tailings Radiation Control Act of 1978 (UMTRCA). The UMTRCA requires that the U.S. Nuclear Regulatory Commission (NRC) concur with the DOE`s remedial action plan (RAP) and certify that the remedial action conducted at the site complies with the standards promulgated by the U.S. Environmental Protection Agency (EPA). The RAP, which includes this summary remedial action selection report (RAS), serves a two-fold purpose. First, it describes the activities proposed by the DOE to accomplish long-term stabilization and control of the residual radioactive materials at the inactive uranium processing site near Falls City, Texas. Second, this document and the remainder of the RAP, upon concurrence and execution by the DOE, the State of Texas, and the NRC, becomes Appendix B of the Cooperative Agreement between the DOE and the State of Texas.

  3. Star-forming regions at the periphery of the supershell surrounding the Cyg OB1 association. I. The star cluster vdB 130 and its ambient gas and dust medium

    E-Print Network [OSTI]

    Sitnik, T G; Lozinskaya, T A; Moiseev, A V; Rastorguev, A S; Tatarnikov, A M; Tatarnikova, A A; Wiebe, D S; Zabolotskikh, M V

    2015-01-01

    Stellar population and the interstellar gas-dust medium in the vicinity of the open star cluster vdB 130 are analysed using optical observations taken with the 6-m telescope of the SAO RAS and the 125-cm telescope of the SAI MSU along with the data of Spitzer and Herschel. Based on proper motions and BV and JHKs 2MASS photometric data, we select additional 36 stars as probable cluster members. Some stars in vdB 130 are classified as B stars. Our estimates of minimum colour excess, apparent distance modulus and the distance are consistent with young age (from 5 to 10 Myrs) of the cluster vdB 130. We suppose the large deviations from the conventional extinction law in the cluster direction, with $R_V$ ~ 4 - 5. The cluster vdB 130 appears to be physically related to the supershell around Cyg OB1, a cometary CO cloud, ionized gas, and regions of infrared emission. There are a few regions of bright mid-infrared emission in the vicinity of vdB 130. The largest of them is also visible on H-alpha and [SII] emission m...

  4. The supergiant shell with triggered star formation in Irr galaxy IC 2574: neutral and ionized gas kinematics

    E-Print Network [OSTI]

    Egorov, O V; Moiseev, A V; Smirnov-Pinchukov, G V

    2014-01-01

    We analyse the ionized gas kinematics in the star formation regions of the supergiant shell (SGS) of the IC 2574 galaxy using observations with the Fabry-Perot interferometer at the 6-m telescope of SAO RAS; the data of the THINGS survey are used to analyze the neutral gas kinematics in the area. We perform the 'derotation' of the H-alpha and HI data cubes and show its efficiency in kinematics analysis. We confirm the SGS expansion velocity 25 km/s obtained by Walter & Brinks (1999) and conclude that the SGS is located at the far side of the galactic disc plane. We determine the expansion velocities, kinematic ages, and the required mechanical energy input rates for four star formation complexes in the walls of the SGS; for the remaining ones we give the limiting values of the above parameters. A comparison with the age and energy input of the complexes' stellar population shows that sufficient energy is fed to all HII regions except one. We discuss in detail the possible nature of this region and that of...

  5. Considerations for Contractile Electroactive Polymeric Materials and Actuators

    SciTech Connect (OSTI)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Charles; Bernasek, Stephen L.; Abelev, Esta

    2009-06-16

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  6. Considerations for Contractile Electroactive Materials and Actuators

    SciTech Connect (OSTI)

    Rasmussen, Lenore; Erickson, Carl J.; Meixler, Lewis D.; Ascione, George; Gentile, Charles A.; Tilson, Carl; Bernasek, Stephen L.; Abelev, Esta

    2010-02-19

    Ras Labs produces electroactive polymer (EAP) based materials and actuators that bend, swell, ripple and now contract (new development) with low electric input. This is an important attribute because of the ability of contraction to produce life-like motion. The mechanism of contraction is not well understood. Radionuclide-labeled experiments were conducted to follow the movement of electrolytes and water in these EAPs when activated. Extreme temperature experiments were performed on the contractile EAPs with very favorable results. One of the biggest challenges in developing these actuators, however, is the electrode-EAP interface because of the pronounced movement of the EAP. Plasma treatments of metallic electrodes were investigated in order to improve the attachment of the embedded electrodes to the EAP material. Surface analysis, adhesive testing, and mechanical testing were conducted to test metal surfaces and metal-polymer interfaces. The nitrogen plasma treatment of titanium produced a strong metal-polymer interface; however, oxygen plasma treatment of both stainless steel and titanium produced even stronger metal-polymer interfaces. Plasma treatment of the electrodes allows for the embedded electrodes and the EAP material of the actuator to work and move as a unit, with no detachment, by significantly improving the metal-polymer interface.

  7. Gaseous Radiochemical Method for Registration of Ionizing Radiation and Its Possible Applications in Science and Industry

    E-Print Network [OSTI]

    S. G. Lebedev; V. E. Yants

    2005-10-06

    This work presents a new possibility of registration of ionizing radiation by the flowing gaseous radiochemical method (FGRM). The specified method uses the property of some solid crystalline lattice materials for a free emission of radioactive isotopes of inert gas atoms formed as a result of nuclear reactions. Generated in an ampoule of the detector, the radioactive inert gases are transported by a gas-carrier into the proportional gas counter of the flowing type, where the decay rate of the radioactive gas species is measured. This quantity is unequivocally related to the flux of particles (neutrons, protons, light and heavy ions) at the location of the ampoule. The method was used to monitor the neutron flux of the pulsed neutron target "RADEX" driven by the linear proton accelerator of INR RAS. Further progress of the FGRM may give rise to possible applications in nuclear physics, astrophysics and medicine, in the nondestructive control of fissionable materials, diagnostics of thermonuclear plasma, monitoring of fluxes and measurement of spectra of bombarding particles.

  8. Concanavalin A: A potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics

    SciTech Connect (OSTI)

    Li, Wen-wen; Yu, Jia-ying; Xu, Huai-long [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)] [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China); Bao, Jin-ku, E-mail: jinkubao@yahoo.com [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)] [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)

    2011-10-22

    Highlights: {yields} ConA induces cancer cell death targeting apoptosis and autophagy. {yields} ConA inhibits cancer cell angiogenesis. {yields} ConA is utilized in pre-clinical and clinical trials. -- Abstract: Concanavalin A (ConA), a Ca{sup 2+}/Mn{sup 2+}-dependent and mannose/glucose-binding legume lectin, has drawn a rising attention for its remarkable anti-proliferative and anti-tumor activities to a variety of cancer cells. ConA induces programmed cell death via mitochondria-mediated, P73-Foxo1a-Bim apoptosis and BNIP3-mediated mitochondrial autophagy. Through IKK-NF-{kappa}B-COX-2, SHP-2-MEK-1-ERK, and SHP-2-Ras-ERK anti-angiogenic pathways, ConA would inhibit cancer cell survival. In addition, ConA stimulates cell immunity and generates an immune memory, resisting to the same genotypic tumor. These biological findings shed light on new perspectives of ConA as a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis in pre-clinical or clinical trials for cancer therapeutics.

  9. Regulation of human hepatocellular carcinoma cells by Spred2 and correlative studies on its mechanism

    SciTech Connect (OSTI)

    Ma, Xiao-Ni [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Liu, Xiao-Yun [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China) [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Center for Disease Control and Prevention, Lanzhou Military Command, Lanzhou 730020 (China); Yang, Yue-Feng [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Xiao, Feng-Jun [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Li, Qing-Fang [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Yan, Jun [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Zhang, Qun-Wei; Wang, Li-Sheng [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Li, Xue-Yan, E-mail: llglixueyan@163.com [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Wang, Hua, E-mail: wanghua@bmi.ac.cn [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)

    2011-07-15

    Highlights: {yields} Hepatocellular carcinoma is inhibited by Spred2 through as yet unclear mechanisms. {yields} We studied the overexpression of Spred2 in cell line and murine tumor models of HCC. {yields} Spred2 inhibited cell proliferation and migration via attenuating ERK signaling. {yields} Spred2 overexpression induced apoptosis via caspase-3 and downregulated Mcl-1. {yields} A Spred2 knockdown markedly induced tumor growth in vivo. -- Abstract: Members of the Spred gene family are negative regulators of the Ras/Raf-1/ERK pathway, which has been associated with several features of the tumor malignancy. However, the effect of Spred genes on hepatocellular carcinoma (HCC) remains uninvestigated. In the present work, we analyzed the in vitro and in vivo effects of Spred2 expression on the hepatic carcinoma cell line, SMMC-7721. In addition to attenuated ERK activation, which inhibited the proliferation and migration of unstimulated and HGF-stimulated SMMC-7721 cells. Adenovirus-mediated Spred2 overexpression induced the activation of caspase-3 and apoptosis, as well as reduced the expression level of Mcl-1. Most importantly, the knockdown of Spred2 markedly enhanced tumor growth in vivo. In conclusion, these results suggest that Spred2 could qualify as a potential therapeutic target in HCC.

  10. Development and application of procedures to evaluate air quality and visibility impacts of low-altitude flying operations

    SciTech Connect (OSTI)

    Liebsch, E.J.

    1990-08-01

    This report describes the development and application of procedures to evaluate the effects of low-altitude aircraft flights on air quality and visibility. The work summarized in this report was undertaken as part of the larger task of assessing the various potential environmental impacts associated with low-altitude military airspaces. Accomplishing the air quality/visibility analysis for the GEIS included (1) development and application of an integrated air quality model and aircraft emissions database specifically for Military Training Route (MTR) or similar flight operations, (2) selection and application of an existing air quality model to analyze the more widespread and less concentrated aircraft emissions from military Operations Areas (MOAs) and Restricted Areas (RAs), and (3) development and application of procedures to assess impacts of aircraft emissions on visibility. Existing air quality models were considered to be inadequate for predicting ground-level concentrations of pollutants emitted by aircraft along MTRs; therefore, the Single-Aircraft Instantaneous Line Source (SAILS) and Multiple-Aircraft Instantaneous Line Source (MAILS) models were developed to estimate potential impacts along MTRs. Furthermore, a protocol was developed and then applied in the field to determine the degree of visibility impairment caused by aircraft engine exhaust plumes. 19 refs., 2 figs., 3 tabs.

  11. ErbB receptors in the biology and pathology of the aerodigestive tract

    SciTech Connect (OSTI)

    Morgan, Sarah [Department of Otolaryngology, University of Pittsburgh School of Medicine, 200 Lothrop Street (United States); Suite 500, Pittsburgh, PA 15213 (United States); Grandis, Jennifer R. [Department of Otolaryngology, University of Pittsburgh School of Medicine, 200 Lothrop Street (United States); Suite 500, Pittsburgh, PA 15213 (United States)], E-mail: jgrandis@pitt.edu

    2009-02-15

    The most common sites of malignancies in the aerodigestive tract include the lung, head and neck and the esophagus. Esophageal adenocarcinomas (EA), esophageal squamous cell carcinomas (ESCC), and squamous cell carcinomas of the head and neck (SCCHN) are the primary focus of this review. Traditional treatment for aerodigestive tract cancers includes primary chemoradiotherapy (CRT) or surgical resection followed by radiation (or CRT). Recent developments in treatment have focused increasingly on molecular targeting strategies including cetuximab (a monoclonal antibody against epidermal growth factor receptor (EGFR)). Cetuximab was FDA approved in 2006 for treatment of SCCHN, underscoring the importance of understanding the biology of these malignancies. EGFR is a member of the ErbB family of growth factor receptor tyrosine kinases. The major pathways activated by ErbB receptors include Ras/Raf/MAPK; PI3K/AKT; PLC{gamma} and STATs, all of which lead to the transcription of target genes that may contribute to aerodigestive tumor progression. This review explores the expression of ErbB receptors in EA, ESCC and SCCHN and the signaling pathways of EGFR in SCCHN.

  12. Time-dependent restricted-active-space self-consistent-field singles method for many-electron dynamics

    SciTech Connect (OSTI)

    Miyagi, Haruhide; Bojer Madsen, Lars [Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C (Denmark)] [Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C (Denmark)

    2014-04-28

    The time-dependent restricted-active-space self-consistent-field singles (TD-RASSCF-S) method is presented for investigating TD many-electron dynamics in atoms and molecules. Adopting the SCF notion from the muticonfigurational TD Hartree-Fock (MCTDHF) method and the RAS scheme (single-orbital excitation concept) from the TD configuration-interaction singles (TDCIS) method, the TD-RASSCF-S method can be regarded as a hybrid of them. We prove that, for closed-shell N{sub e}-electron systems, the TD-RASSCF-S wave function can be fully converged using only N{sub e}/2 + 1 ? M ? N{sub e} spatial orbitals. Importantly, based on the TD variational principle, the converged TD-RASSCF-S wave function with M = N{sub e} is more accurate than the TDCIS wave function. The accuracy of the TD-RASSCF-S approach over the TDCIS is illustrated by the calculation of high-order harmonic generation spectra for one-dimensional models of atomic helium, beryllium, and carbon in an intense laser pulse. The electronic dynamics during the process is investigated by analyzing the behavior of electron density and orbitals. The TD-RASSCF-S method is accurate, numerically tractable, and applicable for large systems beyond the capability of the MCTDHF method.

  13. An association between the risk of ovarian cancer and rare HRAS1 alleles

    SciTech Connect (OSTI)

    Weitzel, J.N.; Patel, J.; Smith, D.M.

    1994-09-01

    The highly polymorphic HRAS1 minisatellite locus just downstream from the proto-oncogene H-ras-1 on chromosome 11p consists of four common progenitor alleles and several dozen rare alleles, which apparently derive from mutations of the progenitors. Mutant alleles of this locus represent a major risk factor for common types of cancer. Rare-sized HRAS1 alleles have been found more frequently in patients with carcinoma of the breast, colorectum, and urinary bladder and acute leukemia, compared to cancer-free controls. This highly significant association (p<1.001) results in a modest relative risk for patients with one rare allele, but the prevalence of this class of mutant alleles implies an important attributable risk: 1 in 11 cancers of the breast, colorectum, and bladder. Therefore, we performed a case-control study using conventional (Southern blot) and PCR-based methods to score HRAS1 alleles in the leukocyte DNA from 59 patients with ovarian cancer, and 51 cancer-free controls. Our preliminary data suggest an increased incidence of rare and intermediate HRAS1 alleles in caucasian ovarian cancer patients (13%) compared to cancer-free controls (4%). These results parallel the allele distribution seen in the large published series, and lend support for a significant association between rare HRAS1 alleles and ovarian cancer predisposition.

  14. PIK3CA is implicated as an oncogene in ovarian cancer

    SciTech Connect (OSTI)

    Shayesteh, Laleh; Lu, Yiling; Kuo, Wen-Lin; Baldocchi, Russell; Godfrey, Tony; Collins, Colin; Pinkel, Daniel; Powell, Bethan; Mills,Gordon B.; Gray, Joe W.

    1998-03-25

    Ovarian cancer is the leading cause of death from gynecological malignancy and the fourth leading cause of cancer death among American women, yet little is known about its molecular aetiology. Studies using comparative genomic hybridization (CGH) have revealed several regions of recurrent, abnormal, DNA sequence copy number that may encode genes involved in the genesis or progression of the disease. One region at 3q26 found to be increased in copy number in approximately 40 percent of ovarian and other cancers contains PIK3CA, which encodes the p110 a catalytic subunit of phosphatidylinositol 3-kinase(PI3-kinase). The association between PIK3CA copy number and PI3-kinase activity makes PIK3CA a candidate oncogene because a broad range of cancer-related functions have been associated with PI3-kinase mediated signaling. These include proliferation, glucose transport and catabolism, cell adhesion, apoptosis, RAS signaling and oncogenic transformation. In addition, downstream effectors of PI3-kinase,AKT1 and AKT2, have been found to be amplified or activated in human tumors, including ovarian cancer. We show here that PIK3CA is frequently increased in copy number in ovarian cancers, that the increased copy number is associated with increased PIK3CA transcription, p110 a protein expression and PI3-kinase activity and that treatment with the PI3-kinase inhibitor LY294002 decreases proliferation and increases apoptosis. Our observations suggest PIK3CA is an oncogene that has an important role in ovarian cancer.

  15. Mechanisms of radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.

    1996-10-01

    In the process of identifying genes differentially expressed in cells exposed ultraviolet radiation, we have identified a transcript having a 26-bp region that is highly conserved in a variety of species including Bacillus circulans, yeast, pumpkin, Drosophila, mouse, and man. When the 5` region (flanking region or UTR) of a gene, the sequence is predominantly in +/+ orientation with respect to the coding DNA strand; while in the coding region and the 3` region (UTR), the sequence is most frequently in the +/-orientation with respect to the coding DNA strand. In two genes, the element is split into two parts; however, in most cases, it is found only once but with a minimum of 11 consecutive nucleotides precisely depicting the original sequence. The element is found in a large number of different genes with diverse functions (from human ras p21 to B. circulans chitonase). Gel shift assays demonstrated the presence of a protein in HeLa cell extracts that binds to the sense and antisense single-stranded consensus oligomers, as well as to the double- stranded oligonucleotide. When double-stranded oligomer was used, the size shift demonstrated as additional protein-oligomer complex larger than the one bound to either sense or antisense single-stranded consensus oligomers alone. It is speculated either that this element binds to protein(s) important in maintaining DNA is a single-stranded orientation for transcription or, alternatively that this element is important in the transcription-coupled DNA repair process.

  16. Piercing the sovereign ceiling: Issues in oil and gas project financing

    SciTech Connect (OSTI)

    Coleman, T.S. [Moody`s Investors Service, New York, NY (United States)

    1998-02-23

    In the oil and gas sector, corporations and governments face huge capital spending requirements in order to transform large resource discoveries into producing, cash-generating assets. A significant portion of this funding is expected to be done on a project finance basis, where bank lenders or bond investors take a secured position in financing a discrete project, with the expectation of being paid back by the cash flows from that project after completion. This trend is increasing demand for crediting rating services to provide credit ratings for these project financings. A key challenge is to analyze and rate credit-worthy projects in countries that have relatively low foreign currency sovereign ceilings due to economic, political, and financial risks. In most cases, the credit ratings for projects financed in currencies outside the host country are capped at the country`s foreign currency ceiling. However, in a few instances, mainly in the oil and gas sector, Moody`s has pierced the foreign currency ceiling or rated certain projects above the sovereign ratings of the countries where they are domiciled. The purpose of this article is to briefly explain some of the qualitative factors and considerations that have allowed Moody`s to pierce the ceiling in the oil and gas sector, with a focus on two recent and noteworthy projects: Ras Laffan Liquefied Natural Gas in Qatar and Petrozuata in Venezuela.

  17. 2011 Remediation Effectiveness Report for the U.S. Department of Energy Oak Ridge Reservation, Oak Ridge, Tennessee - Data and Evaluations

    SciTech Connect (OSTI)

    Bechtel Jacobs

    2011-03-01

    Under the requirements of the Oak Ridge Reservation (ORR) Federal Facility Agreement (FFA) established between the U.S. Department of Energy (DOE), the U.S. Environmental Protection Agency, (EPA) and the Tennessee Department of Environment and Conservation (TDEC) in 1992, all environmental restoration activities on the ORR are performed in accordance with the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). Since the 1990s, the environmental restoration activities have experienced a gradual shift from characterization to remediation. As this has occurred, it has been determined that the assessment of the individual and cumulative performance of all ORR CERCLA remedial actions (RAs) is most effectively tracked in a single document. The Remediation Effectiveness Report (RER) is an FFA document intended to collate all ORR CERCLA decision requirements, compare pre- and post-remediation conditions at CERCLA sites, and present the results of any required post-decision remediation effectiveness monitoring. First issued in 1997, the RER has been reissued annually to update the performance histories of completed actions and to add descriptions of new CERCLA actions. Monitoring information used in the 2011 RER to assess remedy performance was collected and/or compiled by DOE's Water Resources Restoration Program (WRRP). Only data used to assess performance of completed actions are provided. In addition to collecting CERCLA performance assessment data, the WRRP also collects baseline data to be used to gauge the effectiveness of future actions once implemented. These baseline data are maintained in the Oak Ridge Environmental Information System and will be reported in future RERs, as necessary, once the respective actions are completed. However, when insufficient data exist to assess the impact of the RAs, e.g., when the RA was only recently completed, a preliminary evaluation is made of early indicators of effectiveness at the watershed scale, such as contaminant trends at surface water integration points (IFs). Long-term stewardship (LTS) information used in this report is collected, compiled, and tracked by the WRRP in conjunction with the Bechtel Jacobs Company LLC (BJC) Surveillance and Maintenance (S&M) program, the BJC Radiation Protection Organization at East Tennessee Technology Park (ETTP), ETTP Environmental Compliance Program, B&W Y-12 Liquid Waste Treatment Operations, and UT Battelle Facilities Management Division. Additionally, documentation verifying the implementation of administrative land use controls (LUCs) [i.e., property record restrictions, property record notices, zoning notices, and excavation/penetration permit (EPP) program] is also obtained from many sources throughout the fiscal year (FY), including County Register of Deeds offices for property record restrictions and property record notices, City Planning Commission for zoning notices, and BJC project engineers for EPP program verification. Copies of this documentation are obtained by the WRRP and maintained with the project RER files.

  18. Twenty years of sedimentary change and diagenesis, Qatar Peninsula, Arabian Gulf

    SciTech Connect (OSTI)

    Shinn, E.A.

    1988-02-01

    Re-examination in February 1986 of areas studied by the author in 1966 and 1967 revealed rapid rates of sedimentation and diagenesis along the east coast of the Qatar Peninsula. At Ras Um Sa on the north-east side of Qatar, a series of chenier-like beaches and curved spits is building southward under the influence of longshore currents. Beach and spit growth increasingly protects the shoreline and allows tidal flats to form in their lee. As these spits accrete, they become armored on their lagoonal side by beachrock formation. In the last 20 years a spit approximately 1/2 km long has grown, and beachrock has already armored its recurved lagoonal side. At Umm Said on the southeast side of Qatar, seawardly accreting barchan dunes composed of quartz sand have built a 40-km long, 10-km wide sabkha as thick as 30 m. Aerial and ground photographs show that certain dunes near the seaward edge of the sabkha have migrated into the sea and have contributed to shoreline accretion. The observed rate of dune migration suggests that within approximately 100 years the remaining quartz sands will be depleted and blown into the sea because there is no replenishment of the dune field. Subsequently, the area will revert to one of carbonate deposition. Fine-grained dolomite is presently precipitating in interstitial brines in the landwardmost portions of this unusual sabkha. In the geologic record, such a deposit would be an anomalous, porous and permeable, cross-bedded, linear, 30-m thick, dolomite-cemented sandstone body encased in carbonate sediments. A modern model such as the one at Umm Said can provide useful clues for determining the origin of similar deposits in ancient rocks.

  19. US DOE-EM On-Site Disposal Cell Working Group - Fostering Communication On Performance Assessment Challenges

    SciTech Connect (OSTI)

    Seitz, Roger R. [Savannah River Site (SRS), Aiken, SC (United States); Suttora, Linda C. [U.S. Department of Energy, Office of Site Restoration, Germantown, MD (United States); Phifer, Mark [Savannah River Site (SRS), Aiken, SC (United States)

    2014-03-01

    On-site disposal cells are in use and being considered at several U.S. Department of Energy (USDOE) sites as the final disposition for large amounts of waste associated with cleanup of contaminated areas and facilities. These facilities are typically developed with regulatory oversight from States and/or the US Environmental Protection Agency (USEPA) in addition to USDOE. The facilities are developed to meet design standards for disposal of hazardous waste as well as the USDOE performance based standards for disposal of radioactive waste. The involvement of multiple and different regulators for facilities across separate sites has resulted in some differences in expectations for performance assessments and risk assessments (PA/RA) that are developed for the disposal facilities. The USDOE-EM Office of Site Restoration formed a working group to foster improved communication and sharing of information for personnel associated with these Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) disposal cells and work towards more consistent assumptions, as appropriate, for technical and policy considerations related to performance and risk assessments in support of a Record of Decision and Disposal Authorization Statement. The working group holds teleconferences, as needed, focusing on specific topics of interest. The topics addressed to date include an assessment of the assumptions used for performance assessments and risk assessments (PA/RAs) for on-site disposal cells, requirements and assumptions related to assessment of inadvertent intrusion, DOE Manual 435.1-1 requirements, and approaches for consideration of the long-term performance of liners and covers in the context of PAs. The working group has improved communication among the staff and oversight personnel responsible for onsite disposal cells and has provided a forum to identify and resolve common concerns.

  20. TEC protein tyrosine kinase is involved in the Erk signaling pathway induced by HGF

    SciTech Connect (OSTI)

    Li, Feifei; Jiang, Yinan [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China)] [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China); Zheng, Qiping [Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612 (United States)] [Department of Anatomy and Cell Biology, Rush University Medical Center, Chicago, IL 60612 (United States); Yang, Xiaoming [Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 (China)] [Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 (China); Wang, Siying, E-mail: sywang@ahmu.edu.cn [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China)] [Department of Pathophysiology, Anhui Medical University, Hefei 230032 (China)

    2011-01-07

    Research highlights: {yields} TEC is rapidly tyrosine-phosphorylated and activated by HGF-stimulation in vivo or after partial hepatectomy in mice. {yields} TEC enhances the activity of Elk and serum response element (SRE) in HGF signaling pathway in hepatocyte. {yields} TEC promotes hepatocyte proliferation through the Erk-MAPK pathway. -- Abstract: Background/aims: TEC, a member of the TEC family of non-receptor type protein tyrosine kinases, has recently been suggested to play a role in hepatocyte proliferation and liver regeneration. This study aims to investigate the putative mechanisms of TEC kinase regulation of hepatocyte differentiation, i.e. to explore which signaling pathway TEC is involved in, and how TEC is activated in hepatocyte after hepatectomy and hepatocyte growth factor (HGF) stimulation. Methods: We performed immunoprecipitation (IP) and immunoblotting (IB) to examine TEC tyrosine phosphorylation after partial hepatectomy in mice and HGF stimulation in WB F-344 hepatic cells. The TEC kinase activity was determined by in vitro kinase assay. Reporter gene assay, antisense oligonucleotide and TEC dominant negative mutant (TEC{sup KM}) were used to examine the possible signaling pathways in which TEC is involved. The cell proliferation rate was evaluated by {sup 3}H-TdR incorporation. Results: TEC phosphorylation and kinase activity were increased in 1 h after hepatectomy or HGF treatment. TEC enhanced the activity of Elk and serum response element (SRE). Inhibition of MEK1 suppressed TEC phosphorylation. Blocking TEC activity dramatically decreased the activation of Erk. Reduced TEC kinase activity also suppressed the proliferation of WB F-344 cells. These results suggest TEC is involved in the Ras-MAPK pathway and acts between MEK1 and Erk. Conclusions: TEC promotes hepatocyte proliferation and regeneration and is involved in HGF-induced Erk signaling pathway.

  1. Inhibition of the ERK phosphorylation plays a role in terbinafine-induced p21 up-regulation and DNA synthesis inhibition in human vascular endothelial cells

    SciTech Connect (OSTI)

    Ho, P.-Y. [Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei, Taiwan (China); Hsu, S.-P. [Institute of Physiology, College of Medicine, National Taiwan University, Taiwan (China); Liang, Y.-C. [Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan (China); Kuo, M.-L. [Institute of Toxicology, College of Medicine, National Taiwan University, Taiwan (China); Ho, Y.-S. [Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan (China); Lee, W.-S. [Graduate Institute of Neuroscience, Taipei Medical University, Taipei, Taiwan (China); Graduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (China); Department of Physiology, Medical College, Taipei Medical University, Taipei, Taiwan (China)], E-mail: wslee@tmu.edu.tw

    2008-05-15

    Previously, we showed that terbinafine (TB) induces cell-cycle arrest in cultured human umbilical vein endothelial cells (HUVEC) through an up-regulation of the p21 protein. The aim of this study is to delineate the molecular mechanisms underlying TB-induced increase of p21 protein. RT-PCR analysis demonstrated that the mRNA levels of p21 and p53 were increased in the TB-treated HUVEC. The p21 promoter activity was also increased by TB treatment. Transfection of HUVEC with p53 dominant negative (DN) abolished the TB-induced increases of p21 promoter activity and protein level, suggesting that the TB-induced increase of p21 is p53-dependent. Western blot analysis demonstrated that TB decreased the levels of phosphorylated extracellular signal-regulated kinase (ERK). Over-expression of mitogen-activated protein kinase (MEK)-1, the immediate upstream activator kinase of ERK, abolished the TB-induced increases of p21 and p53 protein and decrease of thymidine incorporation. The ERK inhibitor (PD98059) enhanced the TB-induced inhibition of thymidine incorporation into HUVEC. Taken together, these data suggest that the decrease of ERK activity plays a role in the TB-induced up-regulation of p21 in HUVEC. On the other hand, pretreatment of the cells with geranylgeraniol (GGOH), farnesol (FOH), or Ras inhibitor peptide did not affect the TB-induced decrease of thymidine incorporation. Taken together, our results suggest that TB might cause a decrease of MEK, which in turn up-regulates p53 through the inhibition of ERK phosphorylation, and finally causes an increase of p21 expression and cell-cycle arrest.

  2. Lysophosphatidic Acid-induced ERK Activation and Chemotaxis in MC3T3-E1 Preosteoblasts are Independent of EGF Receptor Transactivation

    SciTech Connect (OSTI)

    Karagiosis, Sue A.; Chrisler, William B.; Bollinger, Nikki; Karin, Norman J.

    2009-06-01

    Growing evidence indicates that bone-forming osteoblasts and their progenitors are target cells for the lipid growth factor lysophosphatidic acid (LPA) which is produced by degranulating platelets at sites of injury. LPA is a potent inducer of bone cell migration, proliferation and survival in vitro and an attractive candidate to facilitate preosteoblast chemotaxis during skeletal regeneration in vivo, but the intracellular signaling pathways mediating the effects of this lipid on bone cells are not defined. In this study we measured the ability of LPA to stimulate extracellular signal-related kinase (ERK1/2) in MC3T3-E1 preosteoblastic cells and determined the contribution of this pathway to LPA-stimulated chemotaxis. LPA-treated cells exhibited a bimodal activation of ERK1/2 with maximal phosphorylation at 5 and 60 minutes. The kinetics of ERK1/2 phosphorylation were not coupled to Ras activation or LPA-induced elevations in cytosolic Ca2+. While LPA is coupled to the transactivation of the EGF receptor in many cell types, LPA-stimulated ERK1/2 activation in MC3T3-E1 cells was unaffected by inhibition of EGF receptor function. ERK isoforms rapidly accumulated at nuclear sites in LPA-treated cells, a process that was blocked if ERK1/2 phosphorylation was prevented with the MEK1 inhibitor U0126. Blocking ERK1/2 phosphorylation with U0126 also diminished MC3T3-E1 cell migration and altered the normal disassembly of LPA-induced stress fibers, while the inhibition of EGF receptor function had no effect on LPA-coupled preosteoblast motility. Our results identify ERK1/2 activation as a mediatora mediator of LPA-stimulated MC3T3-E1 cell migration that may be relevant to preosteoblast motility during bone repair in vivo.

  3. Id-1 is induced in MDCK epithelial cells by activated Erk/MAPK pathway in response to expression of the Snail and E47 transcription factors

    SciTech Connect (OSTI)

    Jorda, Mireia [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Vinyals, Antonia [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Marazuela, Anna [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Cubillo, Eva [Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM) and Departamento de Bioquimica (UAM), Madrid (Spain); Olmeda, David [Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM) and Departamento de Bioquimica (UAM), Madrid (Spain); Valero, Eva [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain); Cano, Amparo [Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM) and Departamento de Bioquimica (UAM), Madrid (Spain); Fabra, Angels [IDIBELL-Institut de Recerca Oncologica, Centre d'Oncologia Molecular, Barcelona (Spain)]. E-mail: afabra@idibell.org

    2007-07-01

    Id-1, a member of the helix-loop-helix transcription factor family has been shown to be involved in cell proliferation, angiogenesis and invasion of many types of human cancers. We have previously shown that stable expression of E47 and Snail repressors of the E-cadherin promoter in MDCK epithelial cell line triggers epithelial mesenchymal transition (EMT) concomitantly with changes in gene expression. We show here that both factors activate the Id-1 gene promoter and induce Id-1 mRNA and protein. The upregulation of the Id-1 gene occurs through the transactivation of the promoter by the Erk/MAPK signaling pathway. Moreover, oncogenic Ras is also able to activate Id-1 promoter in MDCK cells in the absence of both E47 and Snail transcription factors. Several transcriptionally active regulatory elements have been identified in the proximal promoter, including AP-1, Sp1 and four putative E-boxes. By EMSA, we only detected an increased binding to Sp1 and AP-1 elements in E47- and Snail-expressing cells. Binding is affected by the treatment of cells with PD 98059 MEK inhibitor, suggesting that MAPK/Erk contributes to the recruitment or assembly of proteins to Id-1 promoter. Small interfering RNA directed against Sp1 reduced Id-1 expression and the upregulation of the promoter, indicating that Sp1 is required for Id-1 induction in E47- and Snail-expressing cells. Our results provide new insights into how some target genes are activated during and/or as a consequence of the EMT triggered by both E47 and Snail transcription factors.

  4. Triptolide, a diterpenoid triepoxide, induces antitumor proliferation via activation of c-Jun NH{sub 2}-terminal kinase 1 by decreasing phosphatidylinositol 3-kinase activity in human tumor cells

    SciTech Connect (OSTI)

    Miyata, Yoshiki [Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Science, School of Pharmacy, Hachioji, Tokyo 192-0392 (Japan); Sato, Takashi [Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Science, School of Pharmacy, Hachioji, Tokyo 192-0392 (Japan)]. E-mail: satotak@ps.toyaku.ac.jp; Ito, Akira [Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Science, School of Pharmacy, Hachioji, Tokyo 192-0392 (Japan)

    2005-11-04

    Triptolide, a diterpenoid triepoxide extracted from the Chinese herb Tripterygium wilfordii Hook f., exerts antitumorigenic actions against several tumor cells, but the intracellular target signal molecule(s) for this antitumorigenesis activity of triptolide remains to be identified. In the present study, we demonstrated that triptolide, in a dose-dependent manner, inhibited the proliferation of human fibrosarcoma HT-1080, human squamous carcinoma SAS, and human uterine cervical carcinoma SKG-II cells. In addition, triptolide was found to decrease phosphatidylinositol 3-kinase (PI3K) activity. A PI3K inhibitor, LY-294002, mimicked the triptolide-induced antiproliferative activity in HT-1080, SAS, and SKG-II cells. There was no change in the activity of Akt or protein kinase C (PKC), both of which are downstream effectors in the PI3K pathway. Furthermore, the phosphorylation of Ras, Raf, and mitogen-activated protein/extracellular signal-regulated kinase 1/2 was not modified in HT-1080 cells treated with triptolide. However, the phosphorylation of c-Jun NH{sub 2}-terminal kinase 1 (JNK1) was found to increase in both triptolide- and LY-294002-treated cells. Furthermore, the triptolide-induced inhibition of HT-1080 cell proliferation was not observed by JNK1 siRNA-treatment. These results provide novel evidence that PI3K is a crucial target molecule in the antitumorigenic action of triptolide. They further suggest a possible triptolide-induced inhibitory signal for tumor cell proliferation that is initiated by the decrease in PI3K activity, which in turn leads to the augmentation of JNK1 phosphorylation via the Akt and/or PKC-independent pathway(s). Moreover, it is likely that the activation of JNK1 is required for the triptolide-induced inhibition of tumor proliferation.

  5. KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1

    SciTech Connect (OSTI)

    Goettel, Jeremy A.; Liang, Dongchun; Hilliard, Valda C.; Edelblum, Karen L.; Broadus, Matthew R.; Gould, Kathleen L.; Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232 ; Hanks, Steven K.; Polk, D. Brent

    2011-02-15

    The extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway is a highly conserved signaling pathway that regulates diverse cellular processes including differentiation, proliferation, and survival. Kinase suppressor of Ras-1 (KSR1) binds each of the three ERK cascade components to facilitate pathway activation. Even though KSR1 contains a C-terminal kinase domain, evidence supporting the catalytic function of KSR1 remains controversial. In this study, we produced recombinant wild-type or kinase-inactive (D683A/D700A) KSR1 proteins in Escherichia coli to test the hypothesis that KSR1 is a functional protein kinase. Recombinant wild-type KSR1, but not recombinant kinase-inactive KSR1, underwent autophosphorylation on serine residue(s), phosphorylated myelin basic protein (MBP) as a generic substrate, and phosphorylated recombinant kinase-inactive MAPK/ERK kinase-1 (MEK1). Furthermore, FLAG immunoprecipitates from KSR1{sup -/-} colon epithelial cells stably expressing FLAG-tagged wild-type KSR1 (+KSR1), but not vector (+vector) or FLAG-tagged kinase-inactive KSR1 (+D683A/D700A), were able to phosphorylate kinase-inactive MEK1. Since TNF activates the ERK pathway in colon epithelial cells, we tested the biological effects of KSR1 in the survival response downstream of TNF. We found that +vector and +D683A/D700A cells underwent apoptosis when treated with TNF, whereas +KSR1 cells were resistant. However, +KSR1 cells were sensitized to TNF-induced cell loss in the absence of MEK kinase activity. These data provide clear evidence that KSR1 is a functional protein kinase, MEK1 is an in vitro substrate of KSR1, and the catalytic activities of both proteins are required for eliciting cell survival responses downstream of TNF.

  6. Azidothymidine and cisplatin increase p14ARF expression in OVCAR-3 ovarian cancer cell line

    SciTech Connect (OSTI)

    Vaskivuo, Liisa; Rysae, Jaana; Koivuperae, Johanna; Myllynen, Paeivi; Vaskivuo, Tommi; Chvalova, Katerina; Serpi, Raisa; Savolainen, Eeva-Riitta; Puistola, Ulla; Vaehaekangas, Kirsi . E-mail: kirsi.vahakangas@uku.fi

    2006-10-01

    p14{sup ARF} tumor suppressor protein regulates p53 by interfering with mdm2-p53 interaction. p14{sup ARF} is activated in response to oncogenic stimuli but little is known of the responses of endogenous p14{sup ARF} to different types of cellular stress or DNA damage. Azidothymidine (AZT) is being tested in several clinical trials as an enhancer of anticancer chemotherapy. However, the knowledge of the relationship between AZT and cellular pathways, e.g. p53 pathway, is very limited. In this study, we show that AZT, cisplatin (CDDP) and docetaxel (DTX) all induce unique molecular responses in OVCAR-3 ovarian carcinoma cells carrying a mutated p53, while in A2780, ovarian carcinoma and MCF-7 breast carcinoma cells with wild type p53, all of these drugs cause similar p53 responses. We found that endogenous p14{sup ARF} protein in OVCAR-3 cells is down-regulated by DTX but induced by AZT and a short CDDP pulse treatment. In HT-29 colon carcinoma cells with a mutated p53, all treatments down-regulated p14{sup ARF} protein. Both CDDP and AZT increased the expression of p14ARF mRNA in OVCAR-3 cells. Differences in cell death induced by these drugs did not explain the differences in protein and mRNA expressions. No increase in the level of either c-Myc or H-ras oncoproteins was seen in OVCAR-3 cells after AZT or CDDP-treatment. These results suggest that p14{sup ARF} can respond to DNA damage without oncogene activation in cell lines without functional p53.

  7. Expression analysis and prognostic significance of the SRA1 gene, in ovarian cancer

    SciTech Connect (OSTI)

    Leoutsakou, Theoni; Talieri, Maroulio; Scorilas, Andreas . E-mail: ascorilas@biol.uoa.gr

    2006-06-02

    The SR-related-CTD-associated-factors (SCAFs) have the ability to interact with the C-terminal domain of the RNA polymerase II, linking this way transcription to splicing. SRA1 (SR-A1) gene, encoding for a human high-molecular weight SCAF protein, is located on chromosome 19, between the IRF3 and the R-RAS oncogene and it has been demonstrated from members of our group that SRA1 is constitutively expressed in most of the human tissues, while it is overexpressed in a subset of ovarian tumors. In this study, we examine the expression of SRA1 gene in 111 ovarian malignant tissues and in the human ovarian carcinoma cell lines OVCAR-3, TOV21-G, and ES-2, using a semi-quantitative RT-PCR method. SRA1 gene was overexpressed in 61/111 (55%) of ovarian carcinomas. This higher expression was positively associated to the size of the tumor (p < 0.001), the grade and the stage of the disease (p = 0.003 and p = 0.006, respectively), and the debulking success (p < 0.001). Kaplan-Meier survival analysis revealed that lower SRA1 expression increases the probability of both the longer overall and the progression free survival of the patients. Multivariate Cox regression analysis revealed that SRA1 may be used as an independent prognostic biomarker in ovarian cancer. Our results suggest that SRA1 is associated with cancer progression and may possibly be characterized as a new marker of unfavorable prognosis for ovarian cancer.

  8. Aquatic Natural Areas Analysis and Evaluation: Oak Ridge Reservation

    SciTech Connect (OSTI)

    Baranski, Dr. Michael J.

    2011-04-01

    This report presents an assessment of the natural area value of eight Aquatic Natural Areas (ANAs) and seven Aquatic Reference Areas (ARAs) on the Oak Ridge Reservation (ORR) in Anderson and Roane Counties in east Tennessee. It follows a previous study in 2009 that analyzed and evaluated terrestrial natural areas on the Reservation. The purpose of both studies was to evaluate and rank those specially designated areas on the Reservation that contain sensitive species, special habitats, and natural area value. Natural areas receive special protections through established statutes, regulations, and policies. The ORR contains 33,542 acres (13,574 ha) administered by the Department of Energy. The surface waters of the Reservation range from 1st-order to 5th-order streams, but the majority of the streams recognized as ANAs and ARAs are 1st- and 2nd-order streams. East Fork Poplar Creek is a 4th-order stream and the largest watershed that drains Reservation lands. All the waters of the Reservation eventually reach the Clinch River on the southern and western boundaries of the ORR. All available information was collected, synthesized, and evaluated. Field observations were made to support and supplement the available information. Geographic information system mapping techniques were used to develop several quantitative attributes about the study areas. Narrative descriptions of each ANA and ARA and tables of numerical data were prepared. Criteria for assessment and evaluation were developed, and eight categories of factors were devised to produce a ranking system. The evaluation factors used in the ranking system were: (A) size of area, (B) percentage of watershed protected, (C) taxa present with protected status, (D) overall biotic diversity, (E) stream features, (F) water quality and use support ratings, (G) disturbance regime, and (H) other factors. Each factor was evaluated on a 5-point ranking scale (0-4), and each area received a composite score, where 32 was the maximum score possible. A highly ranked ANA or ARA is one that is large in size compared to other areas, includes a greater proportion of the watershed within Reservation boundaries, contains a number of status taxa at high densities, exhibits a high overall biodiversity, has very good or excellent habitat and water quality, is well protected and isolated from disturbances, and shows several other characteristics that contribute to natural area value. In this report, the term 'natural area' is loosely defined as a terrestrial or aquatic system that exhibits, or is thought to exhibit, high natural integrity and other significant natural values. The purpose of the present study is to evaluate and rank the currently recognized Aquatic Natural Areas (ANAs) and Aquatic Reference Areas (ARAs) on the Oak Ridge Reservation (ORR) for their natural area value. A previous study (Baranski 2009) analyzed, evaluated, and ranked terrestrial areas (Natural Areas [NAs], Reference Areas [RAs], and Cooperative Management Areas [CMAs]) on the ORR for natural area value, and a precise methodology for natural area evaluation was developed. The present study is intended to be a complement and companion to the terrestrial area study and attempts to employ a similar methodology for aquatic areas so that aquatic and terrestrial areas can be compared on a similar scale. This study specifically develops criteria for assessing the ecological, biodiversity, and natural area importance and significance of aquatic systems on the Reservation in a relevant and consistent manner. The information can be integrated into the Tennessee Natural Heritage Program (http://tn.gov/environment/na/nhp.shtml) system and applied to potential new aquatic areas. Further, the information will be useful in planning, management, and protection efforts on the ORR.

  9. Assssment and Mapping of the Riverine Hydrokinetic Resource in the Continental United States

    SciTech Connect (OSTI)

    Jacobson, Paul T.; Ravens, Thomas M.; Cunningham, Keith W.; Scott, George

    2012-12-14

    The U.S. Department of Energy (DOE) funded the Electric Power Research Institute and its collaborative partners, University of Alaska ? Anchorage, University of Alaska ? Fairbanks, and the National Renewable Energy Laboratory, to provide an assessment of the riverine hydrokinetic resource in the continental United States. The assessment benefited from input obtained during two workshops attended by individuals with relevant expertise and from a National Research Council panel commissioned by DOE to provide guidance to this and other concurrent, DOE-funded assessments of water based renewable energy. These sources of expertise provided valuable advice regarding data sources and assessment methodology. The assessment of the hydrokinetic resource in the 48 contiguous states is derived from spatially-explicit data contained in NHDPlus ?a GIS-based database containing river segment-specific information on discharge characteristics and channel slope. 71,398 river segments with mean annual flow greater than 1,000 cubic feet per second (cfs) mean discharge were included in the assessment. Segments with discharge less than 1,000 cfs were dropped from the assessment, as were river segments with hydroelectric dams. The results for the theoretical and technical resource in the 48 contiguous states were found to be relatively insensitive to the cutoff chosen. Raising the cutoff to 1,500 cfs had no effect on estimate of the technically recoverable resource, and the theoretical resource was reduced by 5.3%. The segment-specific theoretical resource was estimated from these data using the standard hydrological engineering equation that relates theoretical hydraulic power (Pth, Watts) to discharge (Q, m3 s-1) and hydraulic head or change in elevation (??, m) over the length of the segment, where ? is the specific weight of water (9800 N m-3): ??? = ? ? ?? For Alaska, which is not encompassed by NPDPlus, hydraulic head and discharge data were manually obtained from Idaho National Laboratory?s Virtual Hydropower Prospector, Google Earth, and U.S. Geological Survey gages. Data were manually obtained for the eleven largest rivers with average flow rates greater than 10,000 cfs and the resulting estimate of the theoretical resource was expanded to include rivers with discharge between 1,000 cfs and 10,000 cfs based upon the contribution of rivers in the latter flow class to the total estimate in the contiguous 48 states. Segment-specific theoretical resource was aggregated by major hydrologic region in the contiguous, lower 48 states and totaled 1,146 TWh/yr. The aggregate estimate of the Alaska theoretical resource is 235 TWh/yr, yielding a total theoretical resource estimate of 1,381 TWh/yr for the continental US. The technically recoverable resource in the contiguous 48 states was estimated by applying a recovery factor to the segment-specific theoretical resource estimates. The recovery factor scales the theoretical resource for a given segment to take into account assumptions such as minimum required water velocity and depth during low flow conditions, maximum device packing density, device efficiency, and flow statistics (e.g., the 5 percentile flow relative to the average flow rate). The recovery factor also takes account of ?back effects? ? feedback effects of turbine presence on hydraulic head and velocity. The recovery factor was determined over a range of flow rates and slopes using the hydraulic model, HEC-RAS. In the hydraulic modeling, presence of turbines was accounted for by adjusting the Manning coefficient. This analysis, which included 32 scenarios, led to an empirical function relating recovery factor to slope and discharge. Sixty-nine percent of NHDPlus segments included in the theoretical resource estimate for the contiguous 48 states had an estimated recovery factor of zero. For Alaska, data on river slope was not readily available; hence, the recovery factor was estimated based on the flow rate alone. Segment-specific estimates of the theoretical resource were multiplied by the corresponding recovery factor to estimate