National Library of Energy BETA

Sample records for green fluorescent protein

  1. Engineering and Characterization of a Superfolder Green Fluorescent Protein

    SciTech Connect (OSTI)

    Pedelacq,J.; Cabantous, S.; Tran, T.; Terwilliger, T.; Waldo, G.

    2006-01-01

    Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

  2. Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Close, Devin W.; Paul, Craig Don; Langan, Patricia S.; Wilce, Matthew C. J.; Traore, Daouda A. K.; Halfmann, Randal; Rocha, Reginaldo C.; Waldo, Geoffery S.; Payne, Riley J.; Rucker, Joseph B.; et al

    2015-05-08

    In this paper, we describe the engineering and X-ray crystal structure of Thermal Green Protein (TGP), an extremely stable, highly soluble, non-aggregating green fluorescent protein. TGP is a soluble variant of the fluorescent protein eCGP123, which despite being highly stable, has proven to be aggregation-prone. The X-ray crystal structure of eCGP123, also determined within the context of this paper, was used to carry out rational surface engineering to improve its solubility, leading to TGP. The approach involved simultaneously eliminating crystal lattice contacts while increasing the overall negative charge of the protein. Despite intentional disruption of lattice contacts and introduction ofmore » high entropy glutamate side chains, TGP crystallized readily in a number of different conditions and the X-ray crystal structure of TGP was determined to 1.9 Å resolution. The structural reasons for the enhanced stability of TGP and eCGP123 are discussed. We demonstrate the utility of using TGP as a fusion partner in various assays and significantly, in amyloid assays in which the standard fluorescent protein, EGFP, is undesirable because of aberrant oligomerization.« less

  3. Thermal green protein, an extremely stable, nonaggregating fluorescent protein created by structure-guided surface engineering

    SciTech Connect (OSTI)

    Close, Devin W.; Paul, Craig Don; Langan, Patricia S.; Wilce, Matthew C. J.; Traore, Daouda A. K.; Halfmann, Randal; Rocha, Reginaldo C.; Waldo, Geoffery S.; Payne, Riley J.; Rucker, Joseph B.; Prescott, Mark; Bradbury, Andrew R. M.

    2015-05-08

    In this paper, we describe the engineering and X-ray crystal structure of Thermal Green Protein (TGP), an extremely stable, highly soluble, non-aggregating green fluorescent protein. TGP is a soluble variant of the fluorescent protein eCGP123, which despite being highly stable, has proven to be aggregation-prone. The X-ray crystal structure of eCGP123, also determined within the context of this paper, was used to carry out rational surface engineering to improve its solubility, leading to TGP. The approach involved simultaneously eliminating crystal lattice contacts while increasing the overall negative charge of the protein. Despite intentional disruption of lattice contacts and introduction of high entropy glutamate side chains, TGP crystallized readily in a number of different conditions and the X-ray crystal structure of TGP was determined to 1.9 Å resolution. The structural reasons for the enhanced stability of TGP and eCGP123 are discussed. We demonstrate the utility of using TGP as a fusion partner in various assays and significantly, in amyloid assays in which the standard fluorescent protein, EGFP, is undesirable because of aberrant oligomerization.

  4. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

    SciTech Connect (OSTI)

    Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka

    2015-07-31

    Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS{sup SV40}) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesis takes place. GFP-NLS{sup SV40} in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS{sup SV40} formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS{sup SV40} likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS{sup SV40} can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus.

  5. Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein

    SciTech Connect (OSTI)

    Arpino, James A. J. [Cardiff University, Park Place, Cardiff CF10 3AT Wales (United Kingdom); Rizkallah, Pierre J., E-mail: rizkallahp@cardiff.ac.uk [Cardiff University, Heath Park, Cardiff CF14 4XN Wales (United Kingdom); Jones, D. Dafydd, E-mail: rizkallahp@cardiff.ac.uk [Cardiff University, Park Place, Cardiff CF10 3AT Wales (United Kingdom)

    2014-08-01

    The beneficial engineered single-amino-acid deletion variants EGFP{sup D190?} and EGFP{sup A227?} have been studied. Single-amino-acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and prejudiced molecular understanding of mutations that shorten the protein backbone. Single-amino-acid deletion variants of enhanced green fluorescent protein (EGFP) have been identified by directed evolution with the beneficial effect of imparting increased cellular fluorescence. Biophysical characterization revealed that increased functional protein production and not changes to the fluorescence parameters was the mechanism that was likely to be responsible. The structure EGFP{sup D190?} containing a deletion within a loop revealed propagated changes only after the deleted residue. The structure of EGFP{sup A227?} revealed that a flipping mechanism was used to adjust for residue deletion at the end of a ?-strand, with amino acids C-terminal to the deletion site repositioning to take the place of the deleted amino acid. In both variants new networks of short-range and long-range interactions are generated while maintaining the integrity of the hydrophobic core. Both deletion variants also displayed significant local and long-range changes in dynamics, as evident by changes in B factors compared with EGFP. Rather than being detrimental, deletion mutations can introduce beneficial structural effects through altering core protein properties, folding and dynamics, as well as function.

  6. Development of a reverse genetics system to generate a recombinant Ebola virus Makona expressing a green fluorescent protein

    SciTech Connect (OSTI)

    Albariño, César G. Wiggleton Guerrero, Lisa; Lo, Michael K.; Nichol, Stuart T.; Towner, Jonathan S.

    2015-10-15

    Previous studies have demonstrated the potential application of reverse genetics technology in studying a broad range of aspects of viral biology, including gene regulation, protein function, cell entry, and pathogenesis. Here, we describe a highly efficient reverse genetics system used to generate recombinant Ebola virus (EBOV) based on a recent isolate from a human patient infected during the 2014–2015 outbreak in Western Africa. We also rescued a recombinant EBOV expressing a fluorescent reporter protein from a cleaved VP40 protein fusion. Using this virus and an inexpensive method to quantitate the expression of the foreign gene, we demonstrate its potential usefulness as a tool for screening antiviral compounds and measuring neutralizing antibodies. - Highlights: • Recombinant Ebola virus (EBOV) derived from Makona variant was rescued. • New protocol for viral rescue allows 100% efficiency. • Modified EBOV expresses a green fluorescent protein from a VP40-fused protein. • Modified EBOV was tested as tool to screen antiviral compounds and measure neutralizing antibodies.

  7. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  8. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-11-29

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  9. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2012-05-01

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  10. Recombinant fluorescent protein microsphere calibration standard

    DOE Patents [OSTI]

    Nolan, John P.; Nolan, Rhiannon L.; Ruscetti, Teresa; Lehnert, Bruce E.

    2001-01-01

    A method for making recombinant fluorescent protein standard particles for calibration of fluorescence instruments.

  11. Use of green fluorescent fusion protein to track activation of the transcription factor osterix during early osteoblast differentiation

    SciTech Connect (OSTI)

    Tai Guangping; Christodoulou, Ioannis; Bishop, Anne E.; Polak, Julia M. . E-mail: julia.polak@imperial.ac.uk

    2005-08-12

    Osterix (Osx) is a transcription factor required for the differentiation of preosteoblasts into fully functioning osteoblasts. However, the pattern of Osx activation during preosteoblast differentiation and maturation has not been clearly defined. Our aim was to study Osx activation during these processes in osteoblasts differentiating from murine and human embryonic stem cells (ESC). To do this, we constructed an Osx-GFP fusion protein reporter system to track Osx translocation within the cells. The distribution of Osx-GFP at representative stages of differentiation was also investigated by screening primary osteoblasts, mesenchymal stem cells, synoviocytes, and pre-adipocytes. Our experiments revealed that Osx-GFP protein was detectable in the cytoplasm of cultured, differentiated ESC 4 days after plating of enzymatically dispersed embryoid bodies. Osterix-GFP protein became translocated into the nucleus on day 7 following transfer of differentiated ESC to osteogenic medium. After 14 days of differentiation, cells showing nuclear translocation of Osx-GFP formed rudimentary bone nodules that continued to increase in number over the following weeks (through day 21). We also found that Osx translocated into the nuclei of mesenchymal stem cells (C3H10T1/2) and pre-osteoblasts (MC3T3-E1) and showed partial activation in pre-adipocytes (MC3T3-L1). These data suggest that Osx activation occurs at a very early point in the differentiation of the mesenchymal-osteoblastic lineage.

  12. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2015-07-14

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  13. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  14. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2011-06-07

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  15. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

    DOE Patents [OSTI]

    Waldo, Geoffrey S.

    2007-09-18

    The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

  16. Protein- protein interaction detection system using fluorescent protein microdomains

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2010-02-23

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  17. Blue-green phosphor for fluorescent lighting applications

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  18. Protein subcellular localization assays using split fluorescent proteins

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2009-09-08

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  19. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Proteins Scientists manipulate and mimic proteins for use in creating solutions for medicine, sustainable energy, and more Read caption + Los Alamos National Laboratory graduate student, Patricia Langan, changes the properties of a green fluorescent protein in order to create new fluorescent protein variants. Overview of Research and Highlights Scientists at Los Alamos apply a unique collection of tools and expertise to gain a comprehensive understanding of the structure and function of proteins

  20. Mapping protein collapse with single molecule fluorescence and...

    Office of Scientific and Technical Information (OSTI)

    spectroscopy Citation Details In-Document Search Title: Mapping protein collapse with single molecule fluorescence and kinetic synchrotron radiation circular dichroism spectroscopy ...

  1. LucY: A versatile new fluorescent reporter protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Auldridge, Michele E.; Cao, Hongnan; Sen, Saurabh; Franz, Laura P.; Bingman, Craig A.; Yennamalli, Ragothaman M.; Phillips, Jr., George N.; Mead, David; Steinmetz, Eric J.; Michnick, Stephen W.

    2015-04-23

    We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276nm, 377nm, and 460nm and a single emission peak at 530nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrastmore » to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.« less

  2. Yellow fluorescent protein phiYFPv (Phialidium): structure and structure-based mutagenesis

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z. Souslova, Ekaterina; Chudakov, Dmitry M.; Lukyanov, Sergey; Martynov, Vladimir I.; Arhipova, Svetlena; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei

    2013-06-01

    The yellow fluorescent protein phiYFPv with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The yellow fluorescent protein phiYFPv (λ{sub em}{sup max} ≃ 537 nm) with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The latter fluorescent protein is one of only two known cases of naturally occurring proteins that exhibit emission spectra in the yellow–orange range (535–555 nm). Here, the crystal structure of phiYFPv has been determined at 2.05 Å resolution. The ‘yellow’ chromophore formed from the sequence triad Thr65-Tyr66-Gly67 adopts the bicyclic structure typical of fluorophores emitting in the green spectral range. It was demonstrated that perfect antiparallel π-stacking of chromophore Tyr66 and the proximal Tyr203, as well as Val205, facing the chromophore phenolic ring are chiefly responsible for the observed yellow emission of phiYFPv at 537 nm. Structure-based site-directed mutagenesis has been used to identify the key functional residues in the chromophore environment. The obtained results have been utilized to improve the properties of phiYFPv and its homologous monomeric biomarker tagYFP.

  3. Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Deng, Junjing; Vine, David J.; Chen, Si; Nashed, Youssef S. G.; Jin, Qiaoling; Phillips, Nicholas W.; Peterka, Tom; Ross, Rob; Vogt, Stefan; Jacobsen, Chris J.

    2015-02-24

    Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolutionmore » beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub–30-nm resolution structural images and ~90-nm–resolution fluorescence images of several elements in frozen-hydrated green algae. This combined approach offers a way to study the role of trace elements in their structural context.« less

  4. Subunits of highly Fluorescent Protein R-Phycoerythrin as Probes for Cell Imaging and Single-Molecule Detection

    SciTech Connect (OSTI)

    Dragan Isailovic

    2005-12-17

    cells expressing green fluorescent protein (GFP) was reconstructed. Also, fluorescence spectra of E

  5. Rotational order–disorder structure of fluorescent protein FP480

    SciTech Connect (OSTI)

    Pletnev, Sergei; Morozova, Kateryna S.; Verkhusha, Vladislav V.; Dauter, Zbigniew

    2009-09-01

    An analysis of the rotational order–disorder structure of fluorescent protein FP480 is presented. In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

  6. Cyanine-based probe\\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2013-01-15

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  7. green

    National Nuclear Security Administration (NNSA)

    0%2A en Nevada National Security Site operator recognized for green fleet http:www.nnsa.energy.govblognevada-national-security-site-operator-recognized-green-fleet

    The...

  8. X-ray crystal structure and properties of Phanta, a weakly fluorescent photochromic GFP-like protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Paul, Craig Don; Traore, Daouda A. K.; Olsen, Seth; Devenish, Rodney J.; Close, Devin W.; Bell, Toby D. M.; Bradbury, Andrew; Wilce, Matthew C. J.; Prescott, Mark

    2015-04-29

    Phanta is a reversibly photoswitching chromoprotein (ΦF, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (ΦF, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in combination, to the optical properties of Phanta. Single amino acid substitutions at position 193 resulted in proteins with very low ΦF, indicating the importance of this position in controlling the fluorescence efficiency of the variant proteins. The substitution Thr69Val in Phanta was important for supressing the formation of a protonated chromophore species observed in some His193 substituted variants,more » whereas the substitution Gln62Met did not significantly contribute to the useful optical properties of Phanta. X-ray crystal structures for Phanta (2.3 Å), eCGP123T69V (2.0 Å) and eCGP123H193Q (2.2 Å) in their non-photoswitched state were determined, revealing the presence of a cis-coplanar chromophore. We conclude that changes in the hydrogen-bonding network supporting the cis-chromophore, and its contacts with the surrounding protein matrix, are responsible for the low fluorescence emission of eCGP123 variants containing a His193 substitution.« less

  9. Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine

    SciTech Connect (OSTI)

    Pletnev, Vladimir Z. Pletneva, Nadya V.; Lukyanov, Konstantin A.; Souslova, Ekaterina A.; Fradkov, Arkady F.; Chudakov, Dmitry M.; Chepurnykh, Tatyana; Yampolsky, Ilia V.; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei

    2013-09-01

    The crystal structure of the novel red emitting fluorescent protein from lancelet Branchiostoma lanceolatum (Chordata) revealed an unusual five residues cyclic unit comprising Gly58-Tyr59-Gly60 chromophore, the following Phe61 and Tyr62 covalently bound to chromophore Tyr59. A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the ‘core’ structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (λ{sub ex}/λ{sub em} = 502/511 nm) and red laRFP (λ{sub ex}/λ{sub em} ≃ 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-ΔS83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ∼20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C{sup β} atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (

  10. Cyanine-based probe\\tag-peptide pair for fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2010-08-17

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  11. Crystal structure of phototoxic orange fluorescent proteins with α tryptophan-based chromophore

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z.; Sarkisyan, Karen S.; Gorbachev, Dmitry A.; Egorov, Evgeny S.; Mishin, Alexander S.; Lukyanov, Konstantin A.; Dauter, Zbigniew; Pletnev, Sergei

    2015-12-23

    Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. We present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKiller-Orange, at 1.81 and 1.57 Å resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the β-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers.

  12. Crystal structure of phototoxic orange fluorescent proteins with α tryptophan-based chromophore

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pletneva, Nadya V.; Pletnev, Vladimir Z.; Sarkisyan, Karen S.; Gorbachev, Dmitry A.; Egorov, Evgeny S.; Mishin, Alexander S.; Lukyanov, Konstantin A.; Dauter, Zbigniew; Pletnev, Sergei

    2015-12-23

    Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. We present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKiller-Orange, at 1.81 and 1.57 Å resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the β-barrel exterior and enablingmore » transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers.« less

  13. Synthesis and evaluation of radioactive and fluorescent residualizing labels for identifying sites of plasma protein catabolism

    SciTech Connect (OSTI)

    Maxwell, J.L.; Baynes, J.W.; Thorpe, S.R.

    1986-05-01

    Inulin and lactose were each coupled to tyramine by reductive amination with NaBH/sub 3/CN and the tyramine then labeled with /sup 125/I. Dilactitol-/sup 125/I-tyramine (DLT) and inulin-/sup 125/I-tyramine (InTn) were coupled by reductive amination and cyanuric chloride, respectively, to asialofetuin (ASF), fetuin and rat serum albumin (RSA). Attachment of either label had no effect on the circulating half-lives of the proteins. Radioactivity from labeled ASF was recovered in rat liver (> 90%) by 1 h post-injection and remained in liver with half-lives of 2 and 6 days, respectively, for the DLT and InTn labels. Whole body recoveries of radioactivity from DLT- and InTn labels. Whole body recoveries of radioactivity from DLT- and InTn-labeled RSA were 5 and 6.5 days, respectively, again indicating that the larger glycoconjugate label residualized more efficiently in cells following protein degradation. (Lactitol)/sub 2/-N-CH/sub 2/-CH/sub 2/-NH-fluroescein (DLF) was also coupled to ASF by reductive amination and recovered quantitatively in liver at 1 h post-injection. Native ASF was an effective competitor for clearance of DLF-ASF from the circulation. Fluorescent degradation products were retained in liver with a half-life of 1.2 days. Residualizing fluorescent labels should be useful for identification and sorting of cells active in the degradation of plasma proteins.

  14. Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same

    DOE Patents [OSTI]

    Tsien, Roger Y.; Wang, Lei

    2008-07-01

    Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

  15. Structural basis for bathochromic shift of fluorescence in far-red fluorescent proteins eqFP650 and eqFP670

    SciTech Connect (OSTI)

    Pletnev, Sergei; Pletneva, Nadya V.; Souslova, Ekaterina A.; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Vladimir

    2012-09-01

    The crystal structures of the far-red fluorescent proteins eqFP650 and eqFP670 have been solved at 1.8 and 1.6 Å resolution, respectively. This permitted identification of the structural elements responsible for the bathochromic shift in both considered far-red fluorescent proteins. The crystal structures of the far-red fluorescent proteins (FPs) eqFP650 (λ{sub ex}{sup max}/λ{sub em}{sup max} 592/650 nm) and eqFP670 (λ{sub ex}{sup max}/λ{sub em}{sup max} 605/670 nm), the successors of the far-red FP Katushka (λ{sub ex}{sup max}/λ{sub em}{sup max} 588/635 nm), have been determined at 1.8 and 1.6 Å resolution, respectively. An examination of the structures demonstrated that there are two groups of changes responsible for the bathochromic shift of excitation/emission bands of these proteins relative to their predecessor. The first group of changes resulted in an increase of hydrophilicity at the acylimine site of the chromophore due to the presence of one and three water molecules in eqFP650 and eqFP670, respectively. These water molecules provide connection of the chromophore with the protein scaffold via hydrogen bonds causing an ∼15 nm bathochromic shift of the eqFP650 and eqFP670 emission bands. The second group of changes observed in eqFP670 arises from substitution of both Ser143 and Ser158 by asparagines. Asn143 and Asn158 of eqFP670 are hydrogen bonded with each other, as well as with the protein scaffold and with the p-hydroxyphenyl group of the chromophore, resulting in an additional ∼20 nm bathochromic shift of the eqFP670 emission band as compared to eqFP650. The role of the observed structural changes was verified by mutagenesis.

  16. Near-infrared fluorescence glucose sensing based on glucose/galactose-binding protein coupled to 651-Blue Oxazine

    SciTech Connect (OSTI)

    Khan, Faaizah; Pickup, John C.

    2013-08-30

    Highlights: We showed that the NIR fluorophore, 651-Blue Oxazine, is solvatochromic (polarity sensitive). Blue Oxazine was covalently attached to mutants of glucose/galactose-binding protein (GBP). Fluorescence intensity of GBP-Blue Oxazine increased with addition of glucose. Fluorescence from bead-immobilised GBP-Blue Oxazine was detectable through skin in vitro. This shows proof-of-concept for non-invasive glucose sensing using GBP-Blue Oxazine. -- Abstract: Near-infrared (NIR) fluorescent dyes that are environmentally sensitive or solvatochromic are useful tools for protein labelling in in vivo biosensor applications such as glucose monitoring in diabetes since their spectral properties are mostly independent of tissue autofluorescence and light scattering, and they offer potential for non-invasive analyte sensing. We showed that the fluorophore 651-Blue Oxazine is polarity-sensitive, with a marked reduction in NIR fluorescence on increasing solvent polarity. Mutants of glucose/galactose-binding protein (GBP) used as the glucose receptor were site-specifically and covalently labelled with Blue Oxazine using click chemistry. Mutants H152C/A213R and H152C/A213R/L238S showed fluorescence increases of 15% and 21% on addition of saturating glucose concentrations and binding constants of 6 and 25 mM respectively. Fluorescence responses to glucose were preserved when GBP-Blue Oxazine was immobilised to agarose beads, and the beads were excited by NIR light through a mouse skin preparation studied in vitro. We conclude GBP-Blue Oxazine shows proof-of-concept as a non-invasive continuous glucose sensing system.

  17. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Purchasing & Technology Goals 6 & 7: Green Purchasing & Green Technology Our goal is to purchase and use environmentally sustainable products whenever possible and to implement...

  18. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Dougalas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2003-12-09

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  19. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Douglas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2002-09-24

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  20. Simultaneous neuron- and astrocyte-specific fluorescent marking

    SciTech Connect (OSTI)

    Schulze, Wiebke; Hayata-Takano, Atsuko; Kamo, Toshihiko; Nakazawa, Takanobu; Nagayasu, Kazuki; Kasai, Atsushi; Seiriki, Kaoru; Shintani, Norihito; Ago, Yukio; Farfan, Camille; and others

    2015-03-27

    Systematic and simultaneous analysis of multiple cell types in the brain is becoming important, but such tools have not yet been adequately developed. Here, we aimed to generate a method for the specific fluorescent labeling of neurons and astrocytes, two major cell types in the brain, and we have developed lentiviral vectors to express the red fluorescent protein tdTomato in neurons and the enhanced green fluorescent protein (EGFP) in astrocytes. Importantly, both fluorescent proteins are fused to histone 2B protein (H2B) to confer nuclear localization to distinguish between single cells. We also constructed several expression constructs, including a tandem alignment of the neuron- and astrocyte-expression cassettes for simultaneous labeling. Introducing these vectors and constructs in vitro and in vivo resulted in cell type-specific and nuclear-localized fluorescence signals enabling easy detection and distinguishability of neurons and astrocytes. This tool is expected to be utilized for the simultaneous analysis of changes in neurons and astrocytes in healthy and diseased brains. - Highlights: • We develop a method for the specific fluorescent labeling of neurons and astrocytes. • Neuron-specific labeling is achieved using Scg10 and synapsin promoters. • Astrocyte-specific labeling is generated using the minimal GFAP promoter. • Nuclear localization of fluorescent proteins is achieved with histone 2B protein.

  1. Methods and compositions for protein detection using nanoparticle-fluorescent polymer complexes

    DOE Patents [OSTI]

    Rotello, Vincent; Bunz, Uwe; You, Chang-Cheng; Miranda, Oscar; Kim, Ik-Bum

    2011-09-20

    Compositions, methods and related apparatus, as can be used for selective protein detection and identification.

  2. Simultaneous cryo X-ray ptychographic and fluorescence microscopy...

    Office of Scientific and Technical Information (OSTI)

    Accepted Manuscript: Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae Prev Next Title: Simultaneous cryo X-ray ptychographic and fluorescence ...

  3. Constructing polyatomic potential energy surfaces by interpolating diabatic Hamiltonian matrices with demonstration on green fluorescent protein chromophore

    SciTech Connect (OSTI)

    Park, Jae Woo; Rhee, Young Min

    2014-04-28

    Simulating molecular dynamics directly on quantum chemically obtained potential energy surfaces is generally time consuming. The cost becomes overwhelming especially when excited state dynamics is aimed with multiple electronic states. The interpolated potential has been suggested as a remedy for the cost issue in various simulation settings ranging from fast gas phase reactions of small molecules to relatively slow condensed phase dynamics with complex surrounding. Here, we present a scheme for interpolating multiple electronic surfaces of a relatively large molecule, with an intention of applying it to studying nonadiabatic behaviors. The scheme starts with adiabatic potential information and its diabatic transformation, both of which can be readily obtained, in principle, with quantum chemical calculations. The adiabatic energies and their derivatives on each interpolation center are combined with the derivative coupling vectors to generate the corresponding diabatic Hamiltonian and its derivatives, and they are subsequently adopted in producing a globally defined diabatic Hamiltonian function. As a demonstration, we employ the scheme to build an interpolated Hamiltonian of a relatively large chromophore, para-hydroxybenzylidene imidazolinone, in reference to its all-atom analytical surface model. We show that the interpolation is indeed reliable enough to reproduce important features of the reference surface model, such as its adiabatic energies and derivative couplings. In addition, nonadiabatic surface hopping simulations with interpolation yield population transfer dynamics that is well in accord with the result generated with the reference analytic surface. With these, we conclude by suggesting that the interpolation of diabatic Hamiltonians will be applicable for studying nonadiabatic behaviors of sizeable molecules.

  4. Engineering ESPT Pathways Based on Structural Analysis of LSSmKate Red Fluorescent Proteins with Large Stokes Shift

    SciTech Connect (OSTI)

    Piatkevich, K.; Malashkevich, V; Almo, S; Verkhusha, V

    2010-01-01

    LSSmKate1 and LSSmKate2 are monomeric red fluorescent proteins (RFPs) with large Stokes shifts (LSSs), which allows for efficient separation of absorbance and emission maxima, as well as for excitation with conventional two-photon laser sources. These LSSmKates differ by a single amino acid substitution at position 160 and exhibit absorbance maxima around 460 nm, corresponding to a neutral DsRed-like chromophore. However, excitation at 460 nm leads to fluorescence emission above 600 nm. Structures of LSSmKate1 and LSSmKate2, determined at resolutions of 2.0 and 1.5 {angstrom}, respectively, revealed that the predominant DsRed-chromophore configurations are cis for LSSmKate1 but trans for LSSmKate2. Crystallographic and mutagenesis analyses, as well as isotope and temperature dependences, suggest that an excited-state proton transfer (ESPT) is responsible for the LSSs observed in LSSmKates. Hydrogen bonding between the chromophore hydroxyl and Glu160 in LSSmKate1 and a proton relay involving the chromophore tyrosine hydroxyl, Ser158, and the Asp160 carboxylate in LSSmKate2 represent the putative ESPT pathways. Comparisons with mKeima LSS RFP suggest that similar proton relays could be engineered in other FPs. Accordingly, we mutated positions 158 and 160 in several conventional red-shifted FPs, including mNeptune, mCherry, mStrawberry, mOrange, and mKO, and the resulting FP variants exhibited LSS fluorescence emission in a wide range of wavelengths from 560 to 640 nm. These data suggest that different chromophores formed by distinct tripeptides in different environments can be rationally modified to yield RFPs with novel photochemical properties.

  5. Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z.; Shemiakina, Irina I.; Chudakov, Dmitriy M.; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei

    2012-08-10

    The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, {lambda}{sub ex} = 552 nm, {lambda}{sub em} = 578 nm) and its bright far-red fluorescent variant Katushka ({lambda}{sub ex} = 588 nm, {lambda}{sub em} = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 {angstrom} at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f.

  6. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Leases Green Leases Green Lease Policies and Procedures for Lease Acquisition Green Lease Policies and Procedures Policy Memorandum Attachment 1: Green Lease Policies and Procedures for Lease Acquisition Attachment 2: Solicitation for Offers with New and Revised Green Lease Text Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart Attachment 4: Unrevised SFO Paragraphs Reissued

    Purchasing & Technology Goals 6 & 7: Green Purchasing & Green

  7. Analysis of the influence of subcellular localization of the HIV Rev protein on Rev-dependent gene expression by multi-fluorescence live-cell imaging

    SciTech Connect (OSTI)

    Wolff, Horst; Hadian, Kamyar; Ziegler, Manja; Weierich, Claudia; Kramer-Hammerle, Susanne; Kleinschmidt, Andrea; Erfle, Volker; Brack-Werner, Ruth . E-mail: brack@gsf.de

    2006-02-15

    The human immunodeficiency virus Rev protein is a post-transcriptional activator of HIV gene expression. Rev is a nucleocytoplasmic shuttle protein that displays characteristic nuclear/nucleolar subcellular localization in various cell lines. Cytoplasmic localization of Rev occurs under various conditions disrupting Rev function. The goal of this study was to investigate the relationship between localization of Rev and its functional activity in living cells. A triple-fluorescent imaging assay, called AQ-FIND, was established for automatic quantitative evaluation of nucleocytoplasmic distribution of fluorescently tagged proteins. This assay was used to screen 500 rev genes generated by error-prone PCR for Rev mutants with different localization phenotypes. Activities of the Rev mutants were determined with a second quantitative, dual-fluorescent reporter assay. In HeLa cells, the majority of nuclear Rev mutants had activities similar to wild-type Rev. The activities of Rev mutants with abnormal cytoplasmic localization ranged from moderately impaired to nonfunctional. There was no linear correlation between subcellular distribution and levels of Rev activity. In astrocytes, nuclear Rev mutants showed similar impaired activities as the cytoplasmic wild-type Rev. Our data suggest that steady-state subcellular localization is not a primary regulator of Rev activity but may change as a secondary consequence of altered Rev function. The methodologies described here have potential for studying the significance of subcellular localization for functions of other regulatory factors.

  8. Observations and Modeling of the Green Ocean Amazon (GOAMAZON...

    Office of Scientific and Technical Information (OSTI)

    Observations and Modeling of the Green Ocean Amazon (GOAMAZON). Particulate Matter and ... transmittance, elemental composition by energy dispersive x-ray fluorescence, and ionic ...

  9. Simultaneous cryo X-ray ptychographic and fluorescence microscopy...

    Office of Scientific and Technical Information (OSTI)

    ptychographic and fluorescence microscopy of green algae You are accessing a document from the Department of Energy's (DOE) Public Access Gateway for Energy & Science (PAGES). ...

  10. Simultaneous cryo X-ray ptychographic and fluorescence microscopy...

    Office of Scientific and Technical Information (OSTI)

    and fluorescence microscopy of green algae Citation Details In-Document Search ... Visit OSTI to utilize additional information resources in energy science and technology. A ...

  11. Greening Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Transportation Goal 2: Greening Transportation LANL supports and encourages employees to reduce their personal greenhouse gas emissions by offering various commuting and work schedule options. Our goal is to reduce emissions related to employee travel and commuting to and from work by 13 percent. Energy Conservation» Efficient Water Use & Management» High Performance Sustainable Buildings» Greening Transportation» Green Purchasing & Green Technology» Pollution Prevention» Science

  12. Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Purchasing Green Purchasing LANL is committed to purchasing and using environmentally preferable products. Contact Environmental Communication & Public Involvement P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505) 667-0216 Email Purchasing Green Products Our goal is to purchase items that contain recycled content except when the items are not available at a reasonable price or do not meet performance standards. Environmentally preferable means products or services that have a smaller

  13. NREL Discovers Novel Protein Interaction in Green Algae that Suggests New Strategies to Improve Hydrogen Photoproduction (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2011-02-01

    A research team at the National Renewable Energy Laboratory (NREL) discovered a specific interaction between the protein ferredoxin - responsible for distributing reductants from photosynthesis to different metabolic pathways - and the HYDA2 hydrogenase, suggesting a role for HYDA2 in photohydrogen production.

  14. Distinct roles of the photosystem II protein PsbS and zeaxanthin in the regulation of light harvesting in plants revealed by fluorescence lifetime snapshots

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sylak-Glassman, Emily J.; Malnoë, Alizée; De Re, Eleonora; Brooks, Matthew D.; Fischer, Alexandra Lee; Niyogi, Krishna K.; Fleming, Graham R.

    2014-10-01

    The photosystem II (PSII) protein PsbS and the enzyme violaxanthin deepoxidase (VDE) are known to influence the dynamics of energy-dependent quenching (qE), the component of nonphotochemical quenching (NPQ) that allows plants to respond to fast fluctuations in light intensity. Although the absence of PsbS and VDE has been shown to change the amount of quenching, there have not been any measurements that can detect whether the presence of these proteins alters the type of quenching that occurs. The chlorophyll fluorescence lifetime probes the excited-state chlorophyll relaxation dynamics and can be used to determine the amount of quenching as well asmore » whether two different genotypes with the same amount of NPQ have similar dynamics of excited-state chlorophyll relaxation. We measured the fluorescence lifetimes on whole leaves of Arabidopsis thaliana throughout the induction and relaxation of NPQ for wild type and the qE mutants, npq4, which lacks PsbS; npq1, which lacks VDE and cannot convert violaxanthin to zeaxanthin; and npq1 npq4, which lacks both VDE and PsbS. These measurements show that although PsbS changes the amount of quenching and the rate at which quenching turns on, it does not affect the relaxation dynamics of excited chlorophyll during quenching. In addition, the data suggest that PsbS responds not only to ΔpH but also to the Δψ across the thylakoid membrane. In contrast, the presence of VDE, which is necessary for the accumulation of zeaxanthin, affects the excited-state chlorophyll relaxation dynamics.« less

  15. Distinct roles of the photosystem II protein PsbS and zeaxanthin in the regulation of light harvesting in plants revealed by fluorescence lifetime snapshots

    SciTech Connect (OSTI)

    Sylak-Glassman, Emily J.; Malnoë, Alizée; De Re, Eleonora; Brooks, Matthew D.; Fischer, Alexandra Lee; Niyogi, Krishna K.; Fleming, Graham R.

    2014-10-01

    The photosystem II (PSII) protein PsbS and the enzyme violaxanthin deepoxidase (VDE) are known to influence the dynamics of energy-dependent quenching (qE), the component of nonphotochemical quenching (NPQ) that allows plants to respond to fast fluctuations in light intensity. Although the absence of PsbS and VDE has been shown to change the amount of quenching, there have not been any measurements that can detect whether the presence of these proteins alters the type of quenching that occurs. The chlorophyll fluorescence lifetime probes the excited-state chlorophyll relaxation dynamics and can be used to determine the amount of quenching as well as whether two different genotypes with the same amount of NPQ have similar dynamics of excited-state chlorophyll relaxation. We measured the fluorescence lifetimes on whole leaves of Arabidopsis thaliana throughout the induction and relaxation of NPQ for wild type and the qE mutants, npq4, which lacks PsbS; npq1, which lacks VDE and cannot convert violaxanthin to zeaxanthin; and npq1 npq4, which lacks both VDE and PsbS. These measurements show that although PsbS changes the amount of quenching and the rate at which quenching turns on, it does not affect the relaxation dynamics of excited chlorophyll during quenching. In addition, the data suggest that PsbS responds not only to ΔpH but also to the Δψ across the thylakoid membrane. In contrast, the presence of VDE, which is necessary for the accumulation of zeaxanthin, affects the excited-state chlorophyll relaxation dynamics.

  16. Green Roofs

    SciTech Connect (OSTI)

    2004-08-01

    A New Technology Demonstration Publication Green roofs can improve the energy performance of federal buildings, help manage stormwater, reduce airborne emissions, and mitigate the effects of urban heat islands.

  17. Green Leases | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Leases Green Leases Green Lease Policies and Procedures for Lease Acquisition Green Lease Policies and Procedures Policy Memorandum Attachment 1: Green Lease Policies and ...

  18. Angiographic Method Using Green Porphyrinew In Primmate Eyes

    DOE Patents [OSTI]

    Miller, Joan W.; Young, Lucy H.Y.; Gragoudas, Evangelos S.

    1998-01-13

    An angiographic method to observe the condition of blood vessels, including neovasculature in the eyes of living primates using green porphyrins and light at a wavelength of 550-700 nm to effect fluorescence is disclosed.

  19. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, Richard I.; Edwards, Bradley C.; Buchwald, Melvin I.; Gosnell, Timothy R.

    1995-01-01

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement.

  20. Interaction between a plasma membrane-localized ankyrin-repeat protein ITN1 and a nuclear protein RTV1

    SciTech Connect (OSTI)

    Sakamoto, Hikaru; Sakata, Keiko; Kusumi, Kensuke; Kojima, Mikiko; Sakakibara, Hitoshi; Iba, Koh

    2012-06-29

    Highlights: Black-Right-Pointing-Pointer ITN1, a plasma membrane ankyrin protein, interacts with a nuclear DNA-binding protein RTV1. Black-Right-Pointing-Pointer The nuclear transport of RTV1 is partially inhibited by interaction with ITN1. Black-Right-Pointing-Pointer RTV1 can promote the nuclear localization of ITN1. Black-Right-Pointing-Pointer Both overexpression of RTV1 and the lack of ITN1 increase salicylic acids sensitivity in plants. -- Abstract: The increased tolerance to NaCl 1 (ITN1) protein is a plasma membrane (PM)-localized protein involved in responses to NaCl stress in Arabidopsis. The predicted structure of ITN1 is composed of multiple transmembrane regions and an ankyrin-repeat domain that is known to mediate protein-protein interactions. To elucidate the molecular functions of ITN1, we searched for interacting partners using a yeast two-hybrid assay, and a nuclear-localized DNA-binding protein, RTV1, was identified as a candidate. Bimolecular fluorescence complementation analysis revealed that RTV1 interacted with ITN1 at the PM and nuclei in vivo. RTV1 tagged with red fluorescent protein localized to nuclei and ITN1 tagged with green fluorescent protein localized to PM; however, both proteins localized to both nuclei and the PM when co-expressed. These findings suggest that RTV1 and ITN1 regulate the subcellular localization of each other.

  1. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, R.I.; Edwards, B.C.; Buchwald, M.I.; Gosnell, T.R.

    1995-09-05

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement. 6 figs.

  2. Green Manufacturing

    SciTech Connect (OSTI)

    Patten, John

    2013-12-31

    Green Manufacturing Initiative (GMI): The initiative provides a conduit between the university and industry to facilitate cooperative research programs of mutual interest to support green (sustainable) goals and efforts. In addition to the operational savings that greener practices can bring, emerging market demands and governmental regulations are making the move to sustainable manufacturing a necessity for success. The funding supports collaborative activities among universities such as the University of Michigan, Michigan State University and Purdue University and among 40 companies to enhance economic and workforce development and provide the potential of technology transfer. WMU participants in the GMI activities included 20 faculty, over 25 students and many staff from across the College of Engineering and Applied Sciences; the College of Arts and Sciences' departments of Chemistry, Physics, Biology and Geology; the College of Business; the Environmental Research Institute; and the Environmental Studies Program. Many outside organizations also contribute to the GMI's success, including Southwest Michigan First; The Right Place of Grand Rapids, MI; Michigan Department of Environmental Quality; the Michigan Department of Energy, Labor and Economic Growth; and the Michigan Manufacturers Technical Center.

  3. Fluorescent Nanoparticles for Radiation DetectionFluorescent...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find More Like This Return to Search Fluorescent Nanoparticles for Radiation DetectionFluorescent Nanoparticles for Radiation Detection Oak Ridge National Laboratory Contact ORNL ...

  4. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Proteins Protein Engineering, Structure, and Function Los Alamos scientists seek a comprehensive understanding of the structure and function of proteins which can lead to a multitude of possibilities, such as enhancing cellulose degradation for biofuels or creating new therapeutics. Contact Us Tom Terwilliger Laboratory Fellow Email Andrew Bradbury Bioscience Group Leader Email Rebecca McDonald Bioscience Communications Email Los Alamos scientists are developing mosaic proteins that may one day

  5. Green Initiatives and Contracting

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    GSA Is Now Training Contracting Officers In Green Purchasing Green Purchasing for the Federal Acquisition Work Force * introduction to the federal green purchasing program * assists learners with identifying green products * discusses factors that shape federal green purchasing initiatives https://cae.gsa.gov 2 "There's some challenges here" "Environmental Aisle" in the GSA Advantage electronic-purchasing website for federal buyers to find green products Environmental

  6. Going Green to Make the Green

    Broader source: Energy.gov (indexed) [DOE]

    Businesses, LLC "Going Green to Make the Green" November 17, 2009 HEROKEE C N E R G ATION NERGY by ENEWABLE ENERATION Wind Farm Project Location Wind Speeds Measured for 5 Years at ...

  7. Green Communities Grant Program

    Office of Energy Efficiency and Renewable Energy (EERE)

    Note: The Green Communities Grant Program is not currently accepting applications. The application deadline to receive a Green Communities designation was October 23, 2015. For designated...

  8. A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins

    SciTech Connect (OSTI)

    Qin Jie [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Xie Liping [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China)]. E-mail: xielp@zjuem.zju.edu.cn; Zheng Xiangyi [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Wang Yunbin [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Bai Yu [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Shen Huafeng [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Li Longcheng [Department of Urology, University of California San Francisco and Veteran Affairs Medical Center San Francisco, San Francisco, CA (United States); Dahiya, Rajvir [Department of Urology, University of California San Francisco and Veteran Affairs Medical Center San Francisco, San Francisco, CA (United States)

    2007-03-23

    Bladder cancer is the fourth most common cancer in men and ninth most common in women. It has a protracted course of progression and is thus an ideal candidate for chemoprevention strategies and trials. This study was conducted to evaluate the chemopreventive/antiproliferative potential of (-)-epigallocatechin gallate (EGCG, the major phytochemical in green tea) against bladder cancer and its mechanism of action. Using the T24 human bladder cancer cell line, we found that EGCG treatment caused dose- and time-dependent inhibition of cellular proliferation and cell viability, and induced apoptosis. Mechanistically, EGCG inhibits phosphatidylinositol 3'-kinase/Akt activation that, in turn, results in modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells. These findings suggest that EGCG may be an important chemoprevention agent for the management of bladder cancer.

  9. Green Builder Media /Green Builder College | Open Energy Information

    Open Energy Info (EERE)

    Builder Media Green Builder College Jump to: navigation, search Name: Green Builder Media Green Builder College Place: Cincinnati, OH Website: www.greenbuildermag.com...

  10. Fluorescence detection: SPIE volume 743

    SciTech Connect (OSTI)

    Menzel, E.R.

    1987-01-01

    This book contains proceedings arranged into four sessions. They are: Fluorescence spectroscopic techniques; Fluorescence in analysis and materials characterization; Fluorescence in medicine and biochemistry; and Fluorescence in criminalistics.

  11. TFS Green | Open Energy Information

    Open Energy Info (EERE)

    TFS Green Jump to: navigation, search Name: TFS Green Place: United Kingdom Sector: Services Product: TFS Green is the environmental markets desk of Tradition Environmental...

  12. Green Button | Department of Energy

    Energy Savers [EERE]

    Button Challenge The White House Announces the Green Button Challenge Read more Apps for Energy: Green Button Apps for Energy: Green Button The Energy Department challenged...

  13. EPA Green Power Leadership Awards

    Broader source: Energy.gov [DOE]

    The U.S. Environmental Protection Agency (EPA) Green Power Leadership Awards recognize exceptional achievement among EPA Green Power Partners and among green power suppliers.

  14. Green Energy Tax Credit

    Broader source: Energy.gov [DOE]

    In addition to the Green Energy Tax Credit, the Carbon Tax Credit is available. This is the only carbon tax credit in the United States and it provides "certified green energy supply chain...

  15. GreenSun Hawaii

    Broader source: Energy.gov [DOE]

    GreenSun Hawaii is a loan loss reserve fund developed using funds from the The American Recovery and Reinvestment Act of 2009 (ARRA). GreenSun Hawaii partners with banks and credit unions, making...

  16. Greening Gas Stations

    Office of Environmental Management (EM)

    Graduate Student, University of California, Los Angeles Department of Urban Planning eere.energy.gov It's Not Easy Being GREEN * What does it mean to be Green? * Can a Gas Station ...

  17. Green Jobs Training Center

    Broader source: Energy.gov [DOE]

    Provides an overview of the training available through the Green Jobs Training Center including certification courses and the apprenticeship program.

  18. Green Power Purchasing

    Office of Energy Efficiency and Renewable Energy (EERE)

    Note: The green power purchasing goal has a target date of 2011. It has not been increased subsequently.

  19. Green Button | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    The White House Announces the Green Button Challenge The White House Announces the Green Button Challenge Read more Apps for Energy: Green Button Apps for Energy: Green Button The ...

  20. Industrial Green | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Industrial Green Industrial Green Industrial Green - This giant bag may not look green, but it keeps a potent greenhouse gas from being released into the atmosphere. It's part of a system at the Free-Electron Laser that retains sulfur hexafluoride gas when it isn't being used in the FEL's gun test stand. The concept received a 2011 Virginia Governor's Environmental Excellence Program Gold Award. Industrial Green On behalf of work done by Kevin Jordan, a senior engineer in the Free-Electron Laser

  1. Blue-green and green phosphors for lighting applications

    DOE Patents [OSTI]

    Setlur, Anant Achyut; Chandran, Ramachandran Gopi; Henderson, Claire Susan; Nammalwar, Pransanth Kumar; Radkov, Emil

    2012-12-11

    Embodiments of the present techniques provide a related family of phosphors that may be used in lighting systems to generate blue or blue-green light. The phosphors include systems having a general formula of: ((Sr.sub.1-zM.sub.z).sub.1-(x+w)A.sub.wCe.sub.x).sub.3(Al.sub.1-ySi.s- ub.y)O.sub.4+y+3(x-w)F.sub.1-y-3(x-w) (I), wherein 0fluorescent tubes, among others, to produce blue and blue/green light. Further, the phosphors may be used in blends with other phosphors, or in combined lighting systems, to produce white light suitable for illumination.

  2. Sherrell R. Greene

    Broader source: Energy.gov [DOE]

    Sherrell Greene is the Founder and President of Advanced Technology Insights, LLC, (ATI) – a professional skills development, management and technical consulting firm.  ATI provides...

  3. Tribal Green Building

    Broader source: Energy.gov (indexed) [DOE]

    Review eere.energy.gov Public Service of Colorado Ponnequin Wind Farm Tribal Energy Program Review Denver, CO Tribal Green Building Gepetta S. Billie Graduate Intern, Tribal Energy ...

  4. A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Technology - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy ...

  5. Fluorescent optical position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  6. Lumens Placard (Green) | Department of Energy

    Energy Savers [EERE]

    Green) Lumens Placard (Green) File lumensplacard-green.eps More Documents & Publications Lumens Placard (Green) Lumens Placard (Black) Lumens Placard (Black...

  7. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: green apps Type Term Title...

  8. Safe biodegradable fluorescent particles

    DOE Patents [OSTI]

    Martin, Sue I.; Fergenson, David P.; Srivastava, Abneesh; Bogan, Michael J.; Riot, Vincent J.; Frank, Matthias

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  9. Green Living, Green Technologies: Things to Be Thankful For ...

    Broader source: Energy.gov (indexed) [DOE]

    We're making strides in offering "green" power throughout the nation, giving consumers the choice of buying green energy and supporting our mission. Our stakeholders represent a ...

  10. Going green earns Laboratory gold

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Going green earns Laboratory gold Going green earns Laboratory gold The Laboratory's newest facility is its first to achieve both the Leadership in Energy and Environmental Design...

  11. Merchant Green | Open Energy Information

    Open Energy Info (EERE)

    Green Jump to: navigation, search Name: Merchant Green Place: Holstebro, Denmark Zip: DK7500 Sector: Renewable Energy, Wind energy Product: Denmark-based market intelligence firm...

  12. Green Habits | Open Energy Information

    Open Energy Info (EERE)

    Green Habits Jump to: navigation, search Name: Green Habits Place: Houston, Texas Zip: 77008 Sector: Efficiency Product: An Austin, Texas-based energy efficiency consulting firm...

  13. Green Exchange | Open Energy Information

    Open Energy Info (EERE)

    Exchange Jump to: navigation, search Name: Green Exchange Place: New York, New York Zip: NY 10282 Product: String representation "The Green Excha ... es marketplace." is too long....

  14. NREL: Sustainable NREL -Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Purchasing Committed to protecting the natural environment, NREL promotes green purchasing-also referred to as sustainable acquisition-by: Implementing new policies,...

  15. Green Dragon | Open Energy Information

    Open Energy Info (EERE)

    Name: Green Dragon Place: United Kingdom Product: Produces solid green(tm) fuel made from the by-product of Phoenix Fuel(tm)s biodiesel process, another...

  16. Going green earns Laboratory gold

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Going green earns Laboratory gold Going green earns Laboratory gold The Laboratory's newest facility is its first to achieve both the Leadership in Energy and Environmental Design ...

  17. Green Light Pulse Oximeter

    DOE Patents [OSTI]

    Scharf, John Edward

    1998-11-03

    A reflectance pulse oximeter that determines oxygen saturation of hemoglobin using two sources of electromagnetic radiation in the green optical region, which provides the maximum reflectance pulsation spectrum. The use of green light allows placement of an oximetry probe at central body sites (e.g., wrist, thigh, abdomen, forehead, scalp, and back). Preferably, the two green light sources alternately emit light at 560 nm and 577 nm, respectively, which gives the biggest difference in hemoglobin extinction coefficients between deoxyhemoglobin, RHb, and oxyhemoglobin, HbO.sub.2.

  18. Maine Green Power Program

    Office of Energy Efficiency and Renewable Energy (EERE)

    Legislation enacted in 2009 directed the Maine Public Utilities Commission (PUC) to develop a program offering green power as an option to residential and small commercial customers in the state....

  19. Green Colorado Credit Reserve

    Broader source: Energy.gov [DOE]

    The Green Colorado Credit Reserve (GCCR) is a loan loss reserve that was created by the Colorado Energy Office (CEO) to incentivize private lenders in Colorado to make small commercial loans up to ...

  20. Green Button Initiative Growing

    Broader source: Energy.gov [DOE]

    The Green Button initiative, which is the common-sense idea that electricity customers should be able to securely download their own energy usage information from their utility websites, is continuing to gain traction across the country.

  1. USPS Lean Green Teams

    SciTech Connect (OSTI)

    2012-08-01

    Institutional change case study details the U.S. Postal Service's Lean Green Teams, which collaborate across functions to identify and implement low- and no-cost ways to conserve natural resources, purchase fewer consumable products, and reduce waste.

  2. Green Energy Loans

    Office of Energy Efficiency and Renewable Energy (EERE)

    Illinois business owners, non-profit organizations, and local governments seeking loans for certain energy efficiency and renewable energy upgrades may apply for a rate reduction, under the Green...

  3. Tribal Green Building Codes

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    with even amount of white space between photos and header Tribal Green Building Codes Chelsea Chee November 1 3, 2012 SAND 2012---9858C Photos placed in horizontal position with ...

  4. Emerging Green Conference

    Broader source: Energy.gov (indexed) [DOE]

    Green Conference Laura Nereng Sustainability Leader 3M Electronics & Energy Business Group September 23, 2014 2 . All Rights Reserved. 13 October 2015 3M Agenda Intro to 3M, ...

  5. Green Supercomputing at Argonne

    ScienceCinema (OSTI)

    Pete Beckman

    2010-01-08

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing?everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently.

  6. Fluorescent fiber diagnostics

    DOE Patents [OSTI]

    Toeppen, John S.

    1994-10-04

    A fluorescent fiber (13) having a doped core (16) is pumped (11) by light (18) of a relatively short wavelength to produce fluorescence at a longer wavelength that is detected by detector (24). The level of fluorescence is monitored (26) and evaluated to provide information as to the excitation of the fiber (13) or the environment thereof. In particular, the level of intensity of the detected fluorescence may be used to measure the intensity of a light beam (18) passing axially through an optical fiber system (12) (FIG. 1 ), or the intensity of a light beam (46) passing radially through a fluorescent fiber (13) (FIG. 2 ), or the level of a fluid (32) in a tank (31) (FIG. 3 ), or a scintillation event (37) in a fluorescent fiber (13) pumped to produce amplification of the scintillation event (FIG. 4 ).

  7. Fluorescent fiber diagnostics

    DOE Patents [OSTI]

    Toeppen, John S.

    1994-01-01

    A fluorescent fiber (13) having a doped core (16) is pumped (11) by light (18) of a relatively short wavelength to produce fluorescence at a longer wavelength that is detected by detector (24). The level of fluorescence is monitored (26) and evaluated to provide information as to the excitation of the fiber (13) or the environment thereof. In particular, the level of intensity of the detected fluorescence may be used to measure the intensity of a light beam (18) passing axially through an optical fiber system (12) (FIG. 1 ), or the intensity of a light beam (46) passing radially through a fluorescent fiber (13) (FIG. 2 ), or the level of a fluid (32) in a tank (31) (FIG. 3 ), or a scintillation event (37) in a fluorescent fiber (13) pumped to produce amplification of the scintillation event (FIG. 4 ).

  8. Green Supercomputing at Argonne

    ScienceCinema (OSTI)

    Beckman, Pete

    2013-04-19

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing?everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently. Argonne was recognized for green computing in the 2009 HPCwire Readers Choice Awards. More at http://www.anl.gov/Media_Center/News/2009/news091117.html Read more about the Argonne Leadership Computing Facility at http://www.alcf.anl.gov/

  9. Fluorescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electricity & Fuel » Lighting » Fluorescent Lighting Fluorescent Lighting Fluorescent Lighting Fluorescent lamps use 25%-35% of the energy used by incandescent products to provide a similar amount of light. They also last about 10 times longer (7,000-24,000 hours). The two general types of fluorescent lamps are: Compact fluorescent lamps (CFLs) -- commonly found with integral ballasts and screw bases, these are popular lamps often used in household fixtures Fluorescent tube and circline

  10. Fluorescent Tube Lamps

    Broader source: Energy.gov [DOE]

    FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

  11. New Insight on a Familiar Glow | U.S. DOE Office of Science ...

    Office of Science (SC) Website

    Researchers discovered how green fluorescent proteins (center) react with water (shown around the edges of the protein). The water stabilizes the protein's glow. The green glow ...

  12. Green | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Green 08-03-2015 - Verification of Protective Properties is Critical before Using any PPE Read more about 08-03-2015 - Verification of Protective Properties is Critical before Using any PPE 12-28-1999 - Catastrphic Failure of a Questron Q3000 Very High Pressure (VHP) Microwave Oven Vessel Read more about 12-28-1999 - Catastrphic Failure of a Questron Q3000 Very High Pressure (VHP) Microwave Oven Vessel 12-16-1997 - Digital Cameras Reduce Personnel Exposures Read more about 12-16-1997 -

  13. GREENING DOE HEADQUARTERS | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    GREENING DOE HEADQUARTERS GREENING DOE HEADQUARTERS PDF icon GREENING DOE HEADQUARTERS ... Litigation of Direct Interest to DOE" The Fuel Cell Industrial Vehicle Jobs Act (H.R. 1659

  14. 2012 Green Utility Leaders | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 Green Utility Leaders 2012 Green Utility Leaders Ranking the Top Green Power Providers See All Leaders x Renewable Energy Sales Total Customer Participants Customer Participation...

  15. Incognito Green Building, LLC | Open Energy Information

    Open Energy Info (EERE)

    Incognito Green Building, LLC Jump to: navigation, search Logo: Incognito Green Building, LLC Name: Incognito Green Building, LLC Address: 230 Dove Court Place: Santa Paula,...

  16. Hydro Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Jump to: navigation, search Name: Hydro Green Energy Place: Houston, Texas Zip: 77056 Sector: Hydro Product: Hydro Green Energy is a project developer and integrator...

  17. Pioneer Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Jump to: navigation, search Name: Pioneer Green Energy Place: Austin, Texas Sector: Renewable Energy, Solar, Wind energy Product: String representation "Pioneer Green...

  18. Advanced Green Technologies | Open Energy Information

    Open Energy Info (EERE)

    Green Technologies Jump to: navigation, search Name: Advanced Green Technologies Place: Fort Lauderdale, Florida Zip: 33311 Product: Advanced Green Technologies is a US-based...

  19. The Green Building Initiative | Open Energy Information

    Open Energy Info (EERE)

    Green Building Initiative Jump to: navigation, search Name: The Green Building Initiative Place: Portland, Oregon Zip: 97201 Product: The Green Building Initiative works with...

  20. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: energy efficiency Type Term...

  1. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: DOE Type Term Title Author...

  2. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: PV Type Term Title Author...

  3. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: utility scale Type Term...

  4. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: Renewable Energy Type Term...

  5. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Green Button Applications > Posts by term > Green Button Applications Content Group Activity By term Q & A Feeds Term: Desert Sunlight Type Term...

  6. Green Hydrogen Company | Open Energy Information

    Open Energy Info (EERE)

    Hydrogen Company Jump to: navigation, search Logo: Green Hydrogen Company Name: Green Hydrogen Company Abbreviation: GH2 Address: Green Hydrogen Company, Head Office, 9...

  7. Green Week 2011 | National Nuclear Security Administration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Week 2011 NNSA Green Week Thursday, April 21 NNSA Highlights Energy Efficient Vehicles Throughout NNSA Today's Green Week 2011 feature highlights some of the ways NNSA's ...

  8. Thermoelectrics: The New Green Automotive Technology | Department...

    Broader source: Energy.gov (indexed) [DOE]

    (2.09 MB) More Documents & Publications Vehicular Thermoelectrics: A New Green Technology Vehicular Thermoelectrics: A New Green Technology Vehicular Thermoelectrics: The New Green

  9. NIST Green Button Presentation | Department of Energy

    Energy Savers [EERE]

    NIST Green Button Presentation NIST Green Button Presentation Green.Button.webinar.for.DOE.Apps.Energy.pptx (6.34 MB) More Documents & Publications Electricity Advisory ...

  10. Study of diffusion of indocyanine green as a photodynamic dye into skin using backscattering spectroscopy

    SciTech Connect (OSTI)

    Genina, E A; Bashkatov, A N; Tuchin, V V

    2014-07-31

    One of the lines of development of modern medicine is theranostics consisting in simultaneous diagnosis and laser treatment with the use of multifunctional agents such as fluorescent indocyanine green that has photodynamic and photothermal properties. Diffusion of indocyanine green dissolved in water and aqueous solutions of alcohols (glycerol, propylene glycol and ethanol) into the dermis is studied by using backscattering spectroscopy. The coefficients of the dye diffusion into the dermis are obtained for the first time by using these solvents. (laser biophotonics)

  11. Green Infrastructure for Arid Communities

    Broader source: Energy.gov [DOE]

    On March 24, 2015, from 1:00pm – 2:30pm EDT, EPA's Green Infrastructure Program will launch our 2015 Webcast Series with the webinar Green Infrastructure for Arid Communities. This webinar aims to...

  12. Green Airplane | Open Energy Information

    Open Energy Info (EERE)

    Green Airplane Jump to: navigation, search The Green Airplane is an emerging technology for a new airplane design that will save 70% on fuel consumption, and reduce nitric oxide...

  13. Green Circle | Open Energy Information

    Open Energy Info (EERE)

    Circle Jump to: navigation, search Name: Green Circle Place: Florida Zip: 32431 Product: Florida-based wood pellet producer. References: Green Circle1 This article is a stub. You...

  14. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen; Sun, Yiru; Giebink, Noel; Thompson, Mark E.

    2010-08-03

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  15. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen R.; Sun, Yiru; Giebink, Noel; Thompson, Mark E.

    2009-01-06

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  16. NIST Presents Green Button: Technical

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29

    Part 3/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  17. NIST Presents Green Button: Technical

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01

    Part 3/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  18. NIST Presents Green Button: Policy

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01

    Part 2/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  19. NIST Presents Green Button: Intro

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01

    Part 1/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  20. NIST Presents Green Button: Policy

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29

    Part 2/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  1. NIST Presents Green Button: Intro

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29

    Part 1/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  2. Lumens Placard (Green) | Department of Energy

    Energy Savers [EERE]

    Green) Lumens Placard (Green) PDF icon Lumens: The new way to shop for light More Documents & Publications Lumens Placard (Black...

  3. City of Chicago- Green Power Purchasing

    Broader source: Energy.gov [DOE]

    For more green power purchasing program listings, visit the U.S. Department of Energy Green Power Network.

  4. Lumens Placard (Green) | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    lumensplacard-green.eps (2.71 MB) More Documents & Publications Lumens Placard (Green) Lumens Placard (Black) Lumens Placard (Black)

  5. IEA Webinar: Green Gas

    Broader source: Energy.gov [DOE]

    As part of the International Energy Agency (IEA) Bioenergy Internal Webinar Series, the Energy Department’s Bioenergy Technologies Office invites you to join a webinar on Thursday, June 2, 2016, at 8 a.m. Eastern Time. During the webinar, participants will learn about the technologies and feedstocks that can contribute to reaching 100% green gas in the gas grid by 2050. The international webinar—Green Gas—will be presented by Professor Jerry D. Murphy, task leader for IEA Bioenergy Task 37 on Energy from Biogas. IEA is an autonomous international organization that studies energy issues related to reliability, affordability, and sustainability. Instructions on how to join the webinar are below. View the international toll free numbers to find your dial-in number (for the United States and Canada, dial 1-888-289-4573).

  6. ProteinShop: A Tool for Interactive Protein

    Office of Scientific and Technical Information (OSTI)

    ... Also, it can be used to define the minimum and maximum per-atom energy values that will be mapped to ProteinShop's green-yellow-red color ramp, and to select any combination of ...

  7. A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Technology - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced Nuclear

  8. Fluorescent image tracking velocimeter

    DOE Patents [OSTI]

    Shaffer, Franklin D.

    1994-01-01

    A multiple-exposure fluorescent image tracking velocimeter (FITV) detects and measures the motion (trajectory, direction and velocity) of small particles close to light scattering surfaces. The small particles may follow the motion of a carrier medium such as a liquid, gas or multi-phase mixture, allowing the motion of the carrier medium to be observed, measured and recorded. The main components of the FITV include: (1) fluorescent particles; (2) a pulsed fluorescent excitation laser source; (3) an imaging camera; and (4) an image analyzer. FITV uses fluorescing particles excited by visible laser light to enhance particle image detectability near light scattering surfaces. The excitation laser light is filtered out before reaching the imaging camera allowing the fluoresced wavelengths emitted by the particles to be detected and recorded by the camera. FITV employs multiple exposures of a single camera image by pulsing the excitation laser light for producing a series of images of each particle along its trajectory. The time-lapsed image may be used to determine trajectory and velocity and the exposures may be coded to derive directional information.

  9. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are aminomore » acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.« less

  10. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    SciTech Connect (OSTI)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

  11. Green Flag for a New Era in Green Racing

    Office of Energy Efficiency and Renewable Energy (EERE)

    Green Racing initiative aims to leverage the technology innovation of the racing community to develop and transfer cleaner, more efficient fuels and vehicle technologies to consumer vehicles.

  12. Technikon Green Energy Development

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Technikon Green Energy Development November 16, 2009 2 Technikon'sRenewable Energy Testing Center 60,000 sq. ft. Energy Application Validation and Development Laboratory  Formed in 2000 after the closure of McClellan Air Force Base  Operating four major DoD Programs for the US Army  Commercial work:  Energy Projects  Air Emission Studies  Hi-Tech Metals Projects RETC - Reutilization of Government Investment  Testing and Validation Model developed under the 1994-2006 Casting

  13. A Designed Protein Maps Brain Activity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Designed Protein Maps Brain Activity Print A team of scientists from the Howard Hughes ... a fluorescent protein (CaMPARI) that allows the permanent marking of active brain cells. ...

  14. Green Peninsula | Open Energy Information

    Open Energy Info (EERE)

    Peninsula Place: St Austell, Cornwall, England, United Kingdom Zip: PL25 3NL Sector: Wind energy Product: Uk based, Green Peninsula, is a wind power project development service...

  15. Green Enterprises | Open Energy Information

    Open Energy Info (EERE)

    Enterprises Jump to: navigation, search Name: Green Enterprises Place: London, United Kingdom Zip: SW7 1QJ Sector: Carbon Product: As an active participant in the global carbon...

  16. Green Empowerment | Open Energy Information

    Open Energy Info (EERE)

    Empowerment Jump to: navigation, search Name: Green Empowerment Place: Portland, Oregon Zip: 97204 Sector: Renewable Energy Product: Non-profit organisation that promotes...

  17. Green Highland | Open Energy Information

    Open Energy Info (EERE)

    Highland Jump to: navigation, search Name: Green Highland Place: United Kingdom Sector: Hydro Product: Specialises in developing small to medium scale hydro electric schemes....

  18. Green Chicane | Open Energy Information

    Open Energy Info (EERE)

    Chicane Jump to: navigation, search Name: Green Chicane Place: East Horsley, England, United Kingdom Zip: KT24 5AG Sector: Services Product: Surrey based company that invests in...

  19. Green Shakti | Open Energy Information

    Open Energy Info (EERE)

    Shakti Jump to: navigation, search Name: Green Shakti Place: Noida, Delhi (NCT), India Product: A company focused on developing power projects based on ecologically sound and...

  20. Green GECCO | Open Energy Information

    Open Energy Info (EERE)

    GECCO Jump to: navigation, search Name: Green GECCO Place: Germany Sector: Renewable Energy Product: Germany-based joint venture to develop renewable energy projects. References:...

  1. Green Solar | Open Energy Information

    Open Energy Info (EERE)

    Solar Jump to: navigation, search Name: Green Solar Place: Paris, France Zip: 45003 Product: Develops and builds PV systems in Guadeloupe and Mauritius. Coordinates: 48.85693,...

  2. Green Joules | Open Energy Information

    Open Energy Info (EERE)

    Joules Jump to: navigation, search Name: Green Joules Place: Park City, Utah Zip: 80460 Sector: Solar, Wind energy Product: Project developer focussing on wind farms larger than...

  3. Green Biologics | Open Energy Information

    Open Energy Info (EERE)

    Biologics Jump to: navigation, search Name: Green Biologics Place: Oxfordshire, United Kingdom Sector: Biomass, Renewable Energy Product: Oxfordshire-based industrial biotech...

  4. Green Strategies | Open Energy Information

    Open Energy Info (EERE)

    Strategies Jump to: navigation, search Name: Green Strategies Address: 816 Connecticut Ave NW Suite 200 Place: Washington, District of Columbia Zip: 20006 Region: Northeast - NY NJ...

  5. Green Machine | Open Energy Information

    Open Energy Info (EERE)

    Information Name Green Machine Facility Geothermal Power Plant Sector Geothermal energy Location Information Coordinates 45.943161, 24.96676 Loading map......

  6. Innovative Financing for Green Infrastructure

    Broader source: Energy.gov [DOE]

    Topic OverviewFinancing green infrastructure is critical to taking projects from planning to implementation and beyond, including sustaining operations and maintenance. This 90-minute webcast will...

  7. Green Appraisals | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Office (BTO) will work together to enable practicing real estate appraisers to better characterize the value of energy-efficient and other "green" attributes of buildings. ...

  8. Green Button GSA Pilot Project

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    My Data Initiatives * Empowering consumers with secure access to their own personal health, energy, and education data * Blue Button (health info), Green Button (energy), ...

  9. A Designed Protein Maps Brain Activity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Designed Protein Maps Brain Activity A Designed Protein Maps Brain Activity Print Wednesday, 28 October 2015 00:00 A team of scientists from the Howard Hughes Medical Institute's Janelia Research Campus designed and validated via x-ray crystallographic studies a fluorescent protein (CaMPARI) that allows the permanent marking of active brain cells. The protein was then used to study live changes via fluorescence in the active nerve cells in brains of fruit flies, zebrafish, and mice. The Neural

  10. Summary of Green Building Programs

    SciTech Connect (OSTI)

    Not Available

    2002-08-01

    In early 2002, the National Association of Home Builders completed a census of residential green building programs across the United States to assess differences and similarities among programs. This report catalogs different ways that builders participate in residential green building programs.

  11. Integrated fluorescence analysis system

    DOE Patents [OSTI]

    Buican, Tudor N.; Yoshida, Thomas M.

    1992-01-01

    An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.

  12. Magnetic fluorescent lamp

    DOE Patents [OSTI]

    Berman, S.M.; Richardson R.W.

    1983-12-29

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  13. Laser-Induced Fluorescence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Induced Fluorescence - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced Nuclear

  14. GreenCore Capital | Open Energy Information

    Open Energy Info (EERE)

    GreenCore Capital Jump to: navigation, search Logo: GreenCore Capital Name: GreenCore Capital Address: 10509 Vista Sorrento Parkway Place: San Diego, California Zip: 92121 Region:...

  15. Driving Green com | Open Energy Information

    Open Energy Info (EERE)

    Driving Green com Jump to: navigation, search Name: Driving Green.com Place: Melbourne, Florida Zip: 32904 Sector: Vehicles Product: Driving green.com is a website that allows...

  16. GOES on Green | Open Energy Information

    Open Energy Info (EERE)

    GOES on Green Jump to: navigation, search Name: GOES on Green Place: United Kingdom Product: Ethanol project developer building a plant in Tyne & Wear. References: GOES on Green1...

  17. IC Green Energy ICG | Open Energy Information

    Open Energy Info (EERE)

    Green Energy ICG Jump to: navigation, search Name: IC Green Energy (ICG) Place: Tel-Aviv, Israel Zip: 61204 Product: IC Green Energy (ICG), a member of Israel Corp. Group, is a new...

  18. Green Button FAQ | OpenEI Community

    Open Energy Info (EERE)

    Green Button FAQ Home > Groups > Green Button Applications NickL's picture Submitted by NickL(137) Contributor 21 July, 2014 - 10:02 Green Button The greenbuttondata.org site has...

  19. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Last Post sort icon Blog entry Green Button DOE Leading by Example with Green Button, American Energy Data Challenge contest 4 NickL 5 May 2015 - 15:37 Blog entry Green Button The...

  20. GreenTops | Open Energy Information

    Open Energy Info (EERE)

    GreenTops Jump to: navigation, search Name: GreenTops Place: Israel Product: Israel-based assemblers of PV-modules mainly for the agricultural comunity. References: GreenTops1...

  1. Energy Department Wins EPA Green Power Award

    Broader source: Energy.gov [DOE]

    The Environmental Protection Agency honored the Energy Department with the Green Power Leadership Award, recognizing that DOE is one of America’s top green power consumers while also being a leader in advancing the green power market.

  2. Jim J. Green | Department of Energy

    Energy Savers [EERE]

    Jim J. Green About Us Jim J. Green - Lead Small Business Specialist Jim J. Green Jim is the Lead Small Business Specialist, at the U.S. Department of Energy, Office of Small and ...

  3. Localization of human elav-like neuronal protein 1 (Hel-N1) on chromosome 9p21 by chromosome microdissection polymerase chain reaction and fluorescence in situ hybridization

    SciTech Connect (OSTI)

    Han, Jian; Knops, J.F.; Longshore, J.W.; King, P.H.

    1996-08-15

    Hel-N1 is a member of the highly conserved elav family of neuronal genes. It shares considerable sequence homology with HuD, another human member, and both genes are expressed in brain. HuD was recently mapped to chromosome 1p34. Here, we have utilized chromosome microdissection polymerase chain reaction and fluorescence in situ hybridization to map Hel-N1 to chromosome 9p21. The different chromosomal locations of these homologous genes underscore their distinct identities. 10 refs., 2 figs.

  4. City of Columbus- Green Columbus Fund

    Broader source: Energy.gov [DOE]

    The Green Columbus Fund incentivizes sustainable development and redevelopment in Columbus, Ohio. The Fund reimburses private and non-profit developers the application fee for the Green Building...

  5. Green Power Marketing | Open Energy Information

    Open Energy Info (EERE)

    Green Power Marketing Jump to: navigation, search Gearbox installation at Xcel Energy's Ponnequin Wind Farm in Colorado. Photo from Jeroen van Dam, NREL 19257 Green power marketing...

  6. Energy Innovation: Green Button Initiative Empowering Americans...

    Energy Savers [EERE]

    Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money...

  7. Green Automotive Company Inc | Open Energy Information

    Open Energy Info (EERE)

    Company Inc Jump to: navigation, search Name: Green Automotive Company Inc Place: Texas Zip: 75001 Product: Texas-based electric vehicle manufacturer. References: Green Automotive...

  8. GreenTech Construction | Open Energy Information

    Open Energy Info (EERE)

    GreenTech Construction Jump to: navigation, search Name: GreenTech Construction Address: 7591 Perry Rd. Place: Delaware, Ohio Zip: 43015 Sector: Buildings, Efficiency Phone Number:...

  9. GreenHomes America | Open Energy Information

    Open Energy Info (EERE)

    GreenHomes America Jump to: navigation, search Name: GreenHomes America Place: Irvine, CA Website: www.greenhomesamerica.com References: Residential Energy Efficiency Stakeholders...

  10. Energy Innovation: Green Button Initiative Empowering Americans...

    Office of Environmental Management (EM)

    Innovation: Green Button Initiative Empowering Americans to Save Energy and Money Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money February ...

  11. Global Green Growth Institute | Open Energy Information

    Open Energy Info (EERE)

    Growth Institute Redirect page Jump to: navigation, search REDIRECT Green Growth Strategy Support Retrieved from "http:en.openei.orgwindex.php?titleGlobalGreenGrowthIn...

  12. Energy Department Issues Green Building Certification System...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Department Issues Green Building Certification System Final Rule to Support Increased Energy Measurement and Efficient Building Design Energy Department Issues Green...

  13. Green Utility Srl | Open Energy Information

    Open Energy Info (EERE)

    Utility Srl Jump to: navigation, search Name: Green Utility Srl Place: Rome, Italy Zip: 153 Product: Italian PV project developer established by Solon, GESENU and Green Utility...

  14. Green Power Wind Farm | Open Energy Information

    Open Energy Info (EERE)

    Wind Farm Jump to: navigation, search Name Green Power Wind Farm Facility Green Power Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner...

  15. Olympia Green Fuels | Open Energy Information

    Open Energy Info (EERE)

    Green Fuels Jump to: navigation, search Name: Olympia Green Fuels Place: Olympia, Washington State Zip: 98502 Product: Olympia-based developer of biodiesel processing technology...

  16. The Green Roundtable | Open Energy Information

    Open Energy Info (EERE)

    Roundtable Jump to: navigation, search Logo: The Green Roundtable Name: The Green Roundtable Address: 38 Chauncy St. Place: Boston, Massachusetts Zip: 02111 Region: Greater Boston...

  17. Blue Green Capital | Open Energy Information

    Open Energy Info (EERE)

    Green Capital Jump to: navigation, search Name: Blue Green Capital Place: Spain Zip: 8860 Sector: Renewable Energy, Solar Product: String representation "Spanish develop ... their...

  18. Green Mountain Energy Company | Open Energy Information

    Open Energy Info (EERE)

    Company Jump to: navigation, search Name: Green Mountain Energy Company Place: Texas Website: www.greenmountainenergy.com Twitter: @GreenMtnEnergy Facebook: https:...

  19. International Green Power IGP | Open Energy Information

    Open Energy Info (EERE)

    Green Power IGP Jump to: navigation, search Name: International Green Power (IGP) Place: Minneapolis, Minnesota Zip: 55432 Product: Minneapolis-based energy development company....

  20. Green Alliance SEGCR | Open Energy Information

    Open Energy Info (EERE)

    Green Alliance SEGCR Place: Spain Product: Fund management company specialising in clean energy funds. References: Green Alliance SEGCR1 This article is a stub. You can help...

  1. Green Mountain Power Corp | Open Energy Information

    Open Energy Info (EERE)

    835-4672 Outage Map: wss.greenmountainpower.comcus Green Button Access: Implemented Green Button Landing Page: www.efficiencyvermont.com References: EIA Form EIA-861 Final...

  2. Pangea Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Pangea Green Energy Jump to: navigation, search Name: Pangea Green Energy Place: Torino, Italy Zip: 101280 Sector: Renewable Energy Product: Renewable energy project developer...

  3. Hainan Green Islands Power | Open Energy Information

    Open Energy Info (EERE)

    Green Islands Power Jump to: navigation, search Name: Hainan Green Islands Power Place: Hainan Province, China Sector: Solar Product: China-based JV developing on-grid solar...

  4. Shalivahana Green Energy SGEL | Open Energy Information

    Open Energy Info (EERE)

    Shalivahana Green Energy SGEL Jump to: navigation, search Name: Shalivahana Green Energy (SGEL) Place: Secunderabad, Andhra Pradesh, India Zip: 500 003 Sector: Biomass, Hydro...

  5. Boston Green Building | Open Energy Information

    Open Energy Info (EERE)

    References Boston Green Building Retrieved from "http:en.openei.orgwindex.php?titleBostonGreenBuilding&oldid768441" Categories: Organizations Energy Efficiency...

  6. Green Capital Management | Open Energy Information

    Open Energy Info (EERE)

    Capital Management Jump to: navigation, search Name: Green Capital Management Place: London, United Kingdom Zip: SW10 0BB Sector: Services Product: The Green Capital Management...

  7. Tilbury Green Power | Open Energy Information

    Open Energy Info (EERE)

    Name: Tilbury Green Power Place: United Kingdom Sector: Biomass Product: UK based Tilbury Green Power is a 100% subsidiary of Express Energy Holdings, developing biomass fired...

  8. Lousiana Green Fuels LLC | Open Energy Information

    Open Energy Info (EERE)

    Lousiana Green Fuels LLC Jump to: navigation, search Name: Lousiana Green Fuels LLC Place: Louisiana Sector: Biomass Product: Developing a cellulosic biomass-to-ethanol plant in...

  9. Leveraged Green Energy LCC | Open Energy Information

    Open Energy Info (EERE)

    Leveraged Green Energy LCC Jump to: navigation, search Name: Leveraged Green Energy LCC Place: Arlington, Virginia Zip: 22209 Sector: Renewable Energy Product: US-based private...

  10. Nagarjuna Green Power | Open Energy Information

    Open Energy Info (EERE)

    Green Power Jump to: navigation, search Name: Nagarjuna Green Power Place: Andhra Pradesh, India Sector: Biomass Product: Hyderabad-based biomass project developer. References:...

  11. Liberty Green Renewables LLC | Open Energy Information

    Open Energy Info (EERE)

    Green Renewables LLC Jump to: navigation, search Name: Liberty Green Renewables, LLC Place: Georgetown, Indiana Zip: 47122 Sector: Biomass Product: Biomass power plant developer...

  12. Petratherm Enel Green JV | Open Energy Information

    Open Energy Info (EERE)

    Enel Green JV Jump to: navigation, search Name: Petratherm & Enel Green JV Place: Spain Sector: Geothermal energy Product: Spain-based JV geothermal project developer. References:...

  13. Sino Icelandic Green Energy Geothermal Development Corporation...

    Open Energy Info (EERE)

    Icelandic Green Energy Geothermal Development Corporation Jump to: navigation, search Name: Sino-Icelandic Green Energy Geothermal Development Corporation Place: China Sector:...

  14. Green Mountain Wind Farm | Open Energy Information

    Open Energy Info (EERE)

    Wind Farm Jump to: navigation, search Name Green Mountain Wind Farm Facility Green Mountain Wind Farm Sector Wind energy Facility Type Commercial Scale Wind Facility Status In...

  15. Shanghai Green Environmental Protection Energy Company Ltd |...

    Open Energy Info (EERE)

    Green Environmental Protection Energy Company Ltd Jump to: navigation, search Name: Shanghai Green Environmental Protection Energy Company Ltd Place: Shanghai, Shanghai...

  16. PERI Green Economics | Open Energy Information

    Open Energy Info (EERE)

    PERI Green Economics Jump to: navigation, search Tool Summary LAUNCH TOOL Name: PERI Green Economics AgencyCompany Organization: Political Economy Research Institute Sector:...

  17. Solesa Green Power | Open Energy Information

    Open Energy Info (EERE)

    Solesa Green Power Jump to: navigation, search Name: Solesa Green Power Place: Torino, Italy Zip: 10144 Sector: Renewable Energy Product: Turin, Italy-based engineering, design,...

  18. Green Coast Enterprises | Open Energy Information

    Open Energy Info (EERE)

    Coast Enterprises Jump to: navigation, search Name: Green Coast Enterprises Place: New Orleans, LA Website: www.greencoastenterprises.com References: Green Coast Enterprises1...

  19. Massachusetts Green Energy Fund | Open Energy Information

    Open Energy Info (EERE)

    Fund Jump to: navigation, search Logo: Massachusetts Green Energy Fund Name: Massachusetts Green Energy Fund Address: 320 Washington Street, 4th Floor Place: Brookline,...

  20. OpenEI Community - Green Button

    Open Energy Info (EERE)

    by Example with Green Button, American Energy Data Challenge contest 4 http:en.openei.orgcommunityblogdoe-leading-example-green-button-american-energy-data-challenge-contest-...

  1. Gerlach Green Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    Green Energy LLC Jump to: navigation, search Name: Gerlach Green Energy LLC Place: Gerlach, Nevada Sector: Geothermal energy Product: Gerlach has formed an exploration joint...

  2. Gulf Coast Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Jump to: navigation, search Name: Gulf Coast Green Energy Place: Bay City, Texas Zip: 77414 Product: The Texas-based company is the exclusive distributor of...

  3. Green Integrated Design | Open Energy Information

    Open Energy Info (EERE)

    Integrated Design Jump to: navigation, search Logo: Green Integrated Design Name: Green Integrated Design Place: Tempe, Arizona Zip: 85283 Number of Employees: 1-10 Year Founded:...

  4. LED Green Power Inc | Open Energy Information

    Open Energy Info (EERE)

    LED Green Power Inc Jump to: navigation, search Name: LED Green Power Inc Place: Santa Cruz, California Zip: 95060 Product: LED fixture and cartridge manufacturer. References: LED...

  5. City of Scottsdale- Green Building Incentives

    Broader source: Energy.gov [DOE]

    Incentives include expedited plan review, green building inspections, lectures, workshops, a homeowner’s manual, recognition on the city web site, and free promotional green building materials,...

  6. Greening up fossil fuels with carbon sequestration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Greening up fossil fuels with carbon sequestration 1663 Los Alamos science and technology magazine Latest Issue:October 2015 past issues All Issues submit Greening up fossil...

  7. green fleet | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    Bios Congressional Testimony Fact Sheets Newsletters Press Releases Video Gallery Photo Gallery Jobs Apply for Our Jobs Our Jobs Working at NNSA Blog Home green fleet green fleet...

  8. Green supercomputing at Argonne | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green supercomputing at Argonne Share Description Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green...

  9. Trans Tech Green Power | Open Energy Information

    Open Energy Info (EERE)

    Trans Tech Green Power Jump to: navigation, search Name: Trans Tech Green Power Place: India Sector: Biomass Product: Plans to develop biomass projects in Rajasthan. References:...

  10. Green Investment Bank | Open Energy Information

    Open Energy Info (EERE)

    Name: Green Investment Bank Place: United Kingdom Product: UK-based investment bank that will focus on clean energy investment. References: Green Investment Bank1 This article...

  11. European Green Cars Initiative | Open Energy Information

    Open Energy Info (EERE)

    Commission Focus Area: Governance - Planning - Decision-Making Structure Topics: Best Practices Website: www.green-cars-initiative.eupublic The European Green Cars...

  12. GreenTech | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: GreenTech Place: Israel Sector: Services Product: General Financial & Legal Services ( Subsidiary Division ) References: GreenTech1 This article...

  13. Arlington County - Green Building Incentive Program | Department...

    Broader source: Energy.gov (indexed) [DOE]

    State Virginia Program Type Green Building Incentive Summary The Green Building Density Incentive program allows the County Board of Arlington to consider a modification of...

  14. Energy Department Issues Green Building Certification System...

    Energy Savers [EERE]

    Green Building Certification System Final Rule to Support Increased Energy Measurement and Efficient Building Design Energy Department Issues Green Building Certification System ...

  15. Measurement & Verification with Green Button Data | Department...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Measurement & Verification with Green Button Data Measurement & Verification with Green ... Measurement and verification (M&V) focuses on ensuring that the savings from energy ...

  16. Workplace Charging Credit for Green Building Certification |...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Leadership in Energy & Environmental Design (LEED) - Created by the U.S. Green Building Council, LEED is a green building certification where organizations can earn up to 100 ...

  17. Tribal Sustainability - Green Projects in Indian Country

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    * Green Building in Indian Country * Conclusion INTRODUCTION Connecting Green Building to Renewable Energy Power Generation *Distribution *Transmission R & D *Highly technical ...

  18. Vehicular Thermoelectrics: The New Green Technology | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The New Green Technology Vehicular Thermoelectrics: The New Green Technology Presentation given at the 16th Directions in Engine-Efficiency and Emissions Research (DEER) Conference ...

  19. Bioindustry Creates Green Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bioindustry Creates Green Jobs Bioindustry Creates Green Jobs Energy from abundant, renewable, domestic biomass can reduce U.S. dependence on oil, lower impacts on climate, and ...

  20. Thermoelectrics: The New Green Automotive Technology | Department...

    Broader source: Energy.gov (indexed) [DOE]

    (5.35 MB) More Documents & Publications Automotive Thermoelectric Generators and HVAC Vehicular Thermoelectrics: A New Green Technology Thermoelectrics: The New Green Automotive

  1. Vehicular Thermoelectrics: A New Green Technology | Department...

    Broader source: Energy.gov (indexed) [DOE]

    (3.68 MB) More Documents & Publications Thermoelectrics: The New Green Automotive Technology Vehicular Thermoelectrics: A New Green Technology Vehicular Thermoelectrics: The

  2. Green Power Partnership | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    EPA Green Power Partnership is a voluntary program encouraging organizations to use green power as a way to reduce the environmental impacts associated with conventional ...

  3. Green Week 2011 Day 2: NNSA Highlights Green Science Innovations...

    National Nuclear Security Administration (NNSA)

    Each day, the NNSA Green Week website will focus on a different set of stories that highlight the connection between NNSA's commitment to energy efficiency and its efforts to ...

  4. Fluorescent microthermographic imaging

    SciTech Connect (OSTI)

    Barton, D.L.

    1993-09-01

    In the early days of microelectronics, design rules and feature sizes were large enough that sub-micron spatial resolution was not needed. Infrared or IR thermal techniques were available that calculated the object`s temperature from infrared emission. There is a fundamental spatial resolution limitation dependent on the wavelengths of light being used in the image formation process. As the integrated circuit feature sizes began to shrink toward the one micron level, the limitations imposed on IR thermal systems became more pronounced. Something else was needed to overcome this limitation. Liquid crystals have been used with great success, but they lack the temperature measurement capabilities of other techniques. The fluorescent microthermographic imaging technique (FMI) was developed to meet this need. This technique offers better than 0.01{degrees}C temperature resolution and is diffraction limited to 0.3 {mu}m spatial resolution. While the temperature resolution is comparable to that available on IR systems, the spatial resolution is much better. The FMI technique provides better spatial resolution by using a temperature dependent fluorescent film that emits light at 612 nm instead of the 1.5 {mu}m to 12 {mu}m range used by IR techniques. This tutorial starts with a review of blackbody radiation physics, the process by which all heated objects emit radiation to their surroundings, in order to understand the sources of information that are available to characterize an object`s surface temperature. The processes used in infrared thermal imaging are then detailed to point out the limitations of the technique but also to contrast it with the FMI process. The FMI technique is then described in detail, starting with the fluorescent film physics and ending with a series of examples of past applications of FMI.

  5. Fluorescence analyzer for lignin

    DOE Patents [OSTI]

    Berthold, John W.; Malito, Michael L.; Jeffers, Larry

    1993-01-01

    A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

  6. Fluorescent temperature sensor

    DOE Patents [OSTI]

    Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

    2009-03-03

    The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

  7. DOE Science Showcase - Green Energy | OSTI, US Dept of Energy...

    Office of Scientific and Technical Information (OSTI)

    Green Energy DOE Green Energy portal DOE GReen Energy Find green energy-related research and development information through a new online portal, DOE Green Energy. The free public ...

  8. Lean and Green Michigan PACE

    Broader source: Energy.gov [DOE]

    Interested property owners should start by performing an energy audit on their facilities to find the most cost effective energy improvements. Lean & Green Michigan can help customers find a ...

  9. Mandatory Utility Green Power Option

    Broader source: Energy.gov [DOE]

    Beginning January 1, 2002, each electric utility must inform its customers on a quarterly basis of the voluntary option to purchase green power. The details of each utility's program must be...

  10. Green Depot | Open Energy Information

    Open Energy Info (EERE)

    Depot Jump to: navigation, search Name: Green Depot Address: P.O. Box 3096 Place: Santa Monica, California Zip: 90408 Region: Bay Area Website: www.greendepot.org Coordinates:...

  11. Broward County- Green Building Policy

    Broader source: Energy.gov [DOE]

    In October 2008, Board of County Commissioners of Broward County passed a resolution creating the County Green Building Policy. All new County-owned and operated buildings must achieve a minimum...

  12. Green Power Program Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the U.S. Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy (EERE) Green Power Program, which was held on May 26, 2005 in Washington, DC. The mission of ...

  13. Final Green Zia Award Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Zia Award for Environmental Excellence CARLSBAD, N.M., August 16, 2012- The Waste Isolation Pilot Plant (WIPP), the U.S. Department of Energy (DOE) facility known as the ...

  14. Guide to Purchasing Green Power

    Broader source: Energy.gov [DOE]

    The Guide to Purchasing Green Power is intended for organizations that are considering the merits of buying green power as well as those that have decided to buy it and want help doing so. The guide was written for a broad audience, including businesses, government agencies, universities, and all organizations wanting to diversify their energy supply and reduce the environmental impact of their electricity use. The Guide can help with planning an on-site renewable generation project.

  15. Estimates of Green potentials. Applications

    SciTech Connect (OSTI)

    Danchenko, V I

    2003-02-28

    Optimal Cartan-type covers by hyperbolic discs of carriers of Green {alpha}-potentials are obtained in a simply connected domain in the complex plane and estimates of the potentials outside the carriers are presented. These results are applied to problems on the separation of singularities of analytic and harmonic functions. For instance, uniform and integral estimates in terms of Green capacities of components of meromorphic functions are obtained.

  16. X-ray fluorescence mapping

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    X-Ray Microscopy and Imaging: X-ray Fluorescence Mapping Of increasing scientific interest is the detection, quantification and mapping of elemental content of samples, often down...

  17. Fluorescent Lighting | Department of Energy

    Office of Environmental Management (EM)

    - by far the most common form of fluorescent lighting but rarely found in residential buildings -- are much more energy efficient than incandescent lamps and are ideally suited...

  18. Tallahassee Program Encourages Residents to Build Green

    Broader source: Energy.gov [DOE]

    Florida city reduces operating costs by increasing energy efficiency, starting with construction of green buildings.

  19. Launching Green Entrepreneurship in New Hampshire

    Broader source: Energy.gov [DOE]

    Green Launching Pad initiative accelerates funding and expertise to grow environmentally-oriented businesses.

  20. A Designed Protein Maps Brain Activity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Designed Protein Maps Brain Activity Print A team of scientists from the Howard Hughes Medical Institute's Janelia Research Campus designed and validated via x-ray crystallographic studies a fluorescent protein (CaMPARI) that allows the permanent marking of active brain cells. The protein was then used to study live changes via fluorescence in the active nerve cells in brains of fruit flies, zebrafish, and mice. The Neural Basis of Behavior Signals in our brains are propagated with voltage and

  1. A Designed Protein Maps Brain Activity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Designed Protein Maps Brain Activity Print A team of scientists from the Howard Hughes Medical Institute's Janelia Research Campus designed and validated via x-ray crystallographic studies a fluorescent protein (CaMPARI) that allows the permanent marking of active brain cells. The protein was then used to study live changes via fluorescence in the active nerve cells in brains of fruit flies, zebrafish, and mice. The Neural Basis of Behavior Signals in our brains are propagated with voltage and

  2. Free Green Energy GreenWell Power JV | Open Energy Information

    Open Energy Info (EERE)

    GreenWell Power JV Jump to: navigation, search Name: Free Green Energy & GreenWell Power JV Place: Texas Sector: Geothermal energy Product: US-based geothermal electricity...

  3. Green Schools Project Final Report

    SciTech Connect (OSTI)

    Verdict, M.

    2000-09-27

    The Alliance to Save Energy has responded to interest in the Green Schools concept from the New England states of Maine, New Hampshire and Vermont. The Alliance conducted a train-the-trainers workshop in Augusta, Maine March 17--18, 1999. This work is part of a Green Schools replication project leveraged by funds from another source, NORDAX, which contributed $80,000 to provide partial support to staff at the Maine Energy Education Project (MEEP), Vermont Energy Education Program (VEEP), and New Hampshire Governor's Office to develop Green Schools Projects. DOE funds were used to conduct training, develop a network of state and local government, business and school partners to support school efficiency activities in those three states.

  4. Red phosphors for use in high CRI fluorescent lamps

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

    2005-11-15

    Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

  5. Blue-green upconversion laser

    DOE Patents [OSTI]

    Nguyen, D.C.; Faulkner, G.E.

    1990-08-14

    A blue-green laser (450--550 nm) uses a host crystal doped with Tm[sup 3+]. The Tm[sup 3+] is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP. 3 figs.

  6. Blue-green upconversion laser

    DOE Patents [OSTI]

    Nguyen, Dinh C.; Faulkner, George E.

    1990-01-01

    A blue-green laser (450-550 nm) uses a host crystal doped with Tm.sup.3+. The Tm.sup.+ is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP.

  7. Green Power Marketing in the United States. A Status Report ...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: English Subject: green power marketing; utility green pricing programs; renewable energy certificates; renewable electricity; green ...

  8. Azerbaijan-UNEP Green Economy Advisory Services | Open Energy...

    Open Energy Info (EERE)

    Azerbaijan-UNEP Green Economy Advisory Services Jump to: navigation, search Logo: Azerbaijan-UNEP Green Economy Advisory Services Name Azerbaijan-UNEP Green Economy Advisory...

  9. Attachment 1: Green Lease Policies and Procedures for Lease Acquisitio...

    Energy Savers [EERE]

    Attachment 1: Green Lease Policies and Procedures for Lease Acquisition Attachment 1: Green Lease Policies and Procedures for Lease Acquisition PDF icon Green Lease Policies and...

  10. Top Green Energy Technologies Inc | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Technologies Inc Jump to: navigation, search Name: Top Green Energy Technologies Inc Place: Jhunan, Taiwan Zip: 350 Sector: Solar Product: Top Green is a manufacturer...

  11. United Utilities Green Energy Ltd | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Ltd Jump to: navigation, search Name: United Utilities Green Energy Ltd Place: England, United Kingdom Sector: Hydro, Renewable Energy Product: United Utilities Green...

  12. China-UNEP Green Economy Advisory Services | Open Energy Information

    Open Energy Info (EERE)

    UNEP Green Economy Advisory Services Jump to: navigation, search Logo: China-UNEP Green Economy Advisory Services Name China-UNEP Green Economy Advisory Services AgencyCompany...

  13. SEP Success Story: Launching Green Entrepreneurship in New Hampshire...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Launching Green Entrepreneurship in New Hampshire SEP Success Story: Launching Green Entrepreneurship in New Hampshire June 18, 2010 - 9:25am Addthis This summer, five green ...

  14. Residential Network Members Unite to Form Green Bank Network...

    Broader source: Energy.gov (indexed) [DOE]

    The NY Green Bank logo. Residential Network members Connecticut Green Bank and NY Green Bank, a division of Residential Network member New York State Energy Research and ...

  15. Johney Green to Lead NREL Mechanical and Thermal Systems Engineering...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Johney Green to Lead NREL Mechanical and Thermal Systems Engineering Research August 11, 2016 Photo of Johney Green Photo of Johney Green The Energy Department's National Renewable ...

  16. Fluorescent fluid interface position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2004-02-17

    A new fluid interface position sensor has been developed, which is capable of optically determining the location of an interface between an upper fluid and a lower fluid, the upper fluid having a larger refractive index than a lower fluid. The sensor functions by measurement, of fluorescence excited by an optical pump beam which is confined within a fluorescent waveguide where that waveguide is in optical contact with the lower fluid, but escapes from the fluorescent waveguide where that waveguide is in optical contact with the upper fluid.

  17. GreenOrder | Open Energy Information

    Open Energy Info (EERE)

    York Zip: 10016 Product: Founded in 2000, GreenOrder is a sustainability strategy and marketing firm References: GreenOrder1 This article is a stub. You can help OpenEI by...

  18. NC GreenPower Production Incentive

    Broader source: Energy.gov [DOE]

    NC GreenPower, a statewide green power program designed to encourage the use of renewable energy in North Carolina, offers production payments for grid-tied electricity generated by solar, wind, ...

  19. City of Boulder- Green Points Building Program

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Boulder Green Points Building Program is a mandatory residential green building program that requires a builder or homeowner to include a minimum amount of sustainable building components bas...

  20. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Menu You must login in order to post into this group. Recent content The Upshot and Innovation of the Green Button Initiative DOE Leading by Example with Green Button, American...

  1. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Gazette We have the right of way when the light is green and we have the green turn signal VDOT needs to paint yellow stripes on the asphalt on either .... Press the menu...

  2. City of Chicago- Green Building Permit Programs

    Broader source: Energy.gov [DOE]

    The Chicago Department of Buildings (DOB) Green Permit Program provides developers and owners with an incentive to build green by streamlining the permit process timeline for projects which are...

  3. Green Fuels Ltd | Open Energy Information

    Open Energy Info (EERE)

    Green Fuels Ltd Place: Malmesbury, Wiltshire, United Kingdom Zip: SN16 9SG Product: Designer of small-scale and commercial-scale biodiesel plants. References: Green Fuels Ltd1...

  4. Crest Global Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Global Green Energy Jump to: navigation, search Name: Crest Global Green Energy Place: County Durham, England, United Kingdom Zip: SR7 7EU Product: UK-based biofuel company with...

  5. Think Green Global Inc | Open Energy Information

    Open Energy Info (EERE)

    Green Global Inc Jump to: navigation, search Name: Think Green Global, Inc. Place: New York, New York Zip: 10010 Sector: Renewable Energy Product: New York-based investment bank...

  6. T O Green Europe | Open Energy Information

    Open Energy Info (EERE)

    O Green Europe Jump to: navigation, search Name: T-O Green Europe Place: Czech Republic Sector: Biomass, Solar, Wind energy Product: Czech-based JV company established by Theolia...

  7. Green Energy Fund | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    (0.356 millskWh) by S.B. 35 of 2007. This money is collected and distributed through the Green Energy Fund, which supports the following programs: Delmarva Power - Green Energy...

  8. Green Power Developers | Open Energy Information

    Open Energy Info (EERE)

    This article is a stub. You can help OpenEI by expanding it. Green Power Developers Green Power Developers is a leading solar energy company, installing and providing...

  9. Eco Green Solutions | Open Energy Information

    Open Energy Info (EERE)

    Eco Green Solutions Place: Brazil Product: Brazil-based Ecodiesel creditor. References: Eco Green Solutions1 This article is a stub. You can help OpenEI by expanding it. Eco...

  10. green apps | OpenEI Community

    Open Energy Info (EERE)

    green apps Home Dc's picture Submitted by Dc(266) Contributor 27 February, 2015 - 11:36 The Energy Efficiency and Renewable Energy (EERE) blog on Green Button is live blog DOE...

  11. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    login in order to post into this group. Recent content The Upshot and Innovation of the Green Button Initiative DOE Leading by Example with Green Button, American Energy Data...

  12. Voluntary Green Building Standards for Public Buildings

    Broader source: Energy.gov [DOE]

    NOTE: The program described below is a voluntary program that encourages state agencies to consider using green building standard. The State of Alabama does not have mandatory Green Building...

  13. Ramblewood Green: Order (2014-CE-23017)

    Broader source: Energy.gov [DOE]

    DOE ordered Ramblewood Green Ltd. to pay a $8,000 civil penalty after finding Ramblewood Green had failed to certify that certain models of cooking products comply with the applicable energy conservation standards.

  14. What's Green Worth? | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    What's Green Worth? What's Green Worth? July 23, 2015 - 6:12pm Addthis The Self-Help Office Building in Wilmington, N.C., reduced nearly 11,000 in annual energy costs and ...

  15. Australia's Green Vehicle Guide | Open Energy Information

    Open Energy Info (EERE)

    URI: cleanenergysolutions.orgcontentaustralias-green-vehicle-guide,http: Language: English Policies: Regulations Regulations: Fuel Efficiency Standards Related Tools...

  16. CHEVROLET | ELECTRIC | GREEN | SPARK EV | TECHNOLOGY. INNOVATION...

    Open Energy Info (EERE)

    CHEVROLET | ELECTRIC | GREEN | SPARK EV | TECHNOLOGY. INNOVATION & SOLUTIONS | GREENER VEHICLES Home There are currently no posts in this category. Syndicate...

  17. Mapping of the receptor protein-tyrosine kinase 10 to human chromosome 1q21-q23 and mouse chromosome 1H1-5 by fluorescence in situ hybridization

    SciTech Connect (OSTI)

    Edelhoff, S.; Disteche, C.M.; Lai, C.

    1995-01-01

    Receptor protein-tyrosine kinases (PTKs) play a critical role in the transduction of signals important to cell growth, differentiation, and survival. Mutations affecting the expression of receptor PTK genes have been associated with a number of vertebrate and invertebrate developmental abnormalities, and the aberrant regulation of tyrosine phosphorylation is implicated in a variety of neoplasias. One estimate suggests that approximately 100 receptor PTK genes exist in the mammalian genome, about half of which have been identified. The tyro-10 receptor protein-tyrosine kinase, first identified in a PCR-based survey for novel tyrosine kinases in the rat nervous system, defines a new subfamily of PTKs. It exhibits a catalytic domain most closely related to those found in the trk PTK receptor subfamily, which transduces signals for nerve growth factor and the related molecules brain-derived neurotrophic factor (BDNF), neurotrophin-3, and neurotrophin-4 (NT-3 and NT-4). Trk and the related PTK receptors trkB and trkC play a critical role in the neurotrophin-dependent survival of subsets of sensory and motor neurons. The predicted tyro-10 extracellular region is, however, distinct from that of the trk subfamily and is unique except for a domain shared with the blood coagulation factors V and VIII, thought to be involved in phospholipid binding. Although tyro-10 RNA is most abundant in heart and skeletal muscle in the adult rat, it is expressed in a wide variety of tissues, including the developing and mature brain. Tyro-10 appears identical to the murine TKT sequence reported by Karn et al. and exhibits a high degree of similarity with the CaK, DDR, and Nep PTKs. A ligand for tyro-10 has not yet been identified. 10 refs., 1 fig.

  18. Trends in Utility Green Pricing Programs (2005)

    SciTech Connect (OSTI)

    Bird, L.; Brown, E.

    2006-10-01

    This report presents year-end 2005 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs.

  19. Green Power Marketing in the United States. A Status Report ...

    Office of Scientific and Technical Information (OSTI)

    Subject: renewable energy certificates; RECs; energy consumers; electricity; green power marketing; green pricing; renewable energy; electricity markets; utilities; greenhouse gas ...

  20. Fluorescent Lighting Basics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lighting Basics Fluorescent Lighting Basics October 17, 2013 - 5:39pm Addthis Light from a fluorescent lamp is first created by an electric current conducted through an inert gas producing ultraviolet light that is invisible to the human eye. The ultraviolet light in turn interacts with special blends of phosphors coating the interior surface of the fluorescent lamp tube that efficiently converts the invisible light into useful white light. Fluorescent lamps require a special power

  1. Fluorescent Lighting Basics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lighting Basics Fluorescent Lighting Basics October 17, 2013 - 5:39pm Addthis Light from a fluorescent lamp is first created by an electric current conducted through an inert gas producing ultraviolet light that is invisible to the human eye. The ultraviolet light in turn interacts with special blends of phosphors coating the interior surface of the fluorescent lamp tube that efficiently converts the invisible light into useful white light. Fluorescent lamps require a special power

  2. Green Future Double Barrel House

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    University Green Future Double Barrel House DOE Race to Zero Student Competition 2016 Sean Benson Team Leader - Net Zero Energy Design I & II Bachelor of Science in Architecture, Dec 2016 Alexis Borman Net Zero Energy Design II Bachelor of Science in Architecture, May 2016 Christopher Brown AIA COTE, Net Zero Energy Design I & II Bachelor of Science in Architecture, May 2016 Devonta Magee Net Zero Energy Design II Bachelor of Science in Architecture, Aug 2016 Yasmine Parker Net Zero

  3. Sunna 535-nm photo-fluorescent film dosimeter response to different environmental conditions

    SciTech Connect (OSTI)

    Murphy, Mark K.; Kovacs, Andras; Mclaughlin, William L.; Miller, Steven D.); Puhl, James M.

    2003-12-01

    Evaluations on the influence of environmental variabilities on the red fluorescence component of the Sunna Model? photo-fluorescent dosimeter have previously been reported. This present paper describes the environmental effects on the response of the green fluorescence component of the same dosimeter, which is manufactured using the injection molding technique. The results presented include temperature, relative humidity, and light influences both during and after irradiation. The green fluorescence signal shows a significant dependence on irradiation temperature below room temperature at 1%/C. Above room temperature (approximately 24C to 60C), the irradiation temperature effect varies from -0.1-1.0%/C, depending on the absorbed dose level. For facilities with irradiation temperatures between 30-60C and absorbed dose levels above 10 kGy, irradiation temperature effects are minimal. Light-effects results indicate that the dosimeter is influenced by ultraviolet and blue wavelengths during irradiation as well as during the post-irradiation stabilization period (approx. 22 hours), requiring the use of light-tight packaging. Results also show that the dosimeter exhibits negligible effects from ambient moisture during and after irradiation when in the range of 33-95% relative humidity.

  4. Green lights program in China

    SciTech Connect (OSTI)

    Dadi, Zhuo; Hong, Liu

    1996-12-31

    In China`s 9th 5-year plan (1996-2000), the Chinese government has placed high priority on energy conservation. The China Green Lights Program (CGLP) is listed as one of the key projects of energy conservation. The basic strategy of the CGLP is to mobilise all of the potential contributors to participate in the program, and to use market signals and supplementary non-market instruments to facilitate its implementation. Governmental funds and loans will be used as seed money to attract private participation in the program. The program contains the following elements: (1) Information dissemination to educate the public on the economic and other values of the program and to provide CGLP information to increase consumer awareness and, as a result, increase the demand for energy-efficient lighting systems. (2) Development of standards and codes for lighting systems, establishment of product specifications, and enforcement of product standards. (3) Development of quality certification and labelling system to provide assurances to consumers that the products they are purchasing will meet their performance and cost saving expectations. (4) Highlighted support and financing for production technology development and production capacity expansion. (5) Demonstration and pilot projects to boost consumer confidence in green lighting systems and to demonstrate new production technologies and processes. (6) International co-operation to expand the international exchange and absorb advanced technology and experience for implementation of the China Green Lights Program.

  5. Dose Response and Post-irradiation Characteristics of the Sunna 535-nm Photo-Fluorescent Film Dosimeter

    SciTech Connect (OSTI)

    Murphy, Mark K.; Kovacs, Andras; Miller, Steven D.; Mclaughlin, William L.

    2003-06-09

    Results of characterization studies on one of the first versions of the Sunna photo-fluorescent dosimeter ? have previously been reported, and describe the performance of the red fluorescence component. This present paper describes dose response and post-irradiation characteristics of the green fluorescence component from the same dosimeter film (Sunna Model ?), which is manufactured using the injection molding technique. This production method may supply batch sizes on the order of 1 million dosimeter film elements while maintaining a signal precision (1?) on the order of ?1% without the need to correct for variability of film thickness. The dosimeter is a 1-cm by 3-cm polymeric film of 0.5-mm thickness that emits green fluorescence at intensities increasing almost linearly with dose. The data presented include dose response, post-irradiation growth, heat treatment, dosimeter aging, dose rate dependence, energy dependence, dose fractionation, variation of response within a batch, and the stability of the fluorimeter response. The results indicate that, as a routine dosimeter, the green signal provides a broad range of response at food irradiation (0.3 to 5 kGy), medical sterilization (5 to 40 kGy), and polymer cross-linking (40 to 250 kGy) dose levels.

  6. green

    National Nuclear Security Administration (NNSA)

    vehicle fleets in the nation.

    Competing with more than 38,000 public fleets, National Security Technologies' (NSTec)Fleet, Fuel & Equipment Service operation...

  7. Covered Product Category: Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  8. High throughput protein production screening

    DOE Patents [OSTI]

    Beernink, Peter T.; Coleman, Matthew A.; Segelke, Brent W.

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  9. Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo

    SciTech Connect (OSTI)

    Kim, Eun-Sung; Yang, Seung-Woo; Hong, Dong-Ki; Kim, Woo-Taek; Kim, Ho-Guen; Lee, Sang-Kyou

    2010-01-29

    Non-viral gene delivery is a safe and suitable alternative to viral vector-mediated delivery to overcome the immunogenicity and tumorigenesis associated with viral vectors. Using the novel, human-origin Hph-1 protein transduction domain that can facilitate the transduction of protein into cells, we developed a new strategy to deliver naked DNA in vitro and in vivo. The new DNA delivery system contains Hph-1-GAL4 DNA-binding domain (DBD) fusion protein and enhanced green fluorescent protein (EGFP) reporter plasmid that includes the five repeats of GAL4 upstream activating sequence (UAS). Hph-1-GAL4-DBD protein formed complex with plasmid DNA through the specific interaction between GAL4-DBD and UAS, and delivered into the cells via the Hph-1-PTD. The pEGFP DNA was successfully delivered by the Hph-1-GAL4 system, and the EGFP was effectively expressed in mammalian cells such as HeLa and Jurkat, as well as in Bright Yellow-2 (BY-2) plant cells. When 10 {mu}g of pEGFP DNA was intranasally administered to mice using Hph-1-GAL4 protein, a high level of EGFP expression was detected throughout the lung tissue for 7 days. These results suggest that an Hph-1-PTD-mediated DNA delivery strategy may be an useful non-viral DNA delivery system for gene therapy and DNA vaccines.

  10. National Green Building Standard Analysis

    SciTech Connect (OSTI)

    NAHB Research Center, Upper Marlboro, Maryland

    2012-07-01

    DOE's Building America Program is a research and development program to improve the energy performance of new and existing homes. The ultimate goal of the Building America Program is to achieve examples of cost-effective, energy efficient solutions for all U.S. climate zones. Periodic maintenance of an ANSI standard by review of the entire document and action to revise or reaffirm it on a schedule not to exceed five years is required by ANSI. In compliance, a consensus group has once again been formed and the National Green Building Standard is currently being reviewed to comply with the periodic maintenance requirement of an ANSI standard.

  11. Greening up fossil fuels with carbon sequestration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Greening up fossil fuels with carbon sequestration 1663 Los Alamos science and technology magazine Latest Issue:July 2016 past issues All Issues » submit Greening up fossil fuels with carbon sequestration Researchers make progress fighting climate change by capturing carbon dioxide from power plants and storing it deep underground in geological reservoirs March 25, 2013 Greening up fossil fuels with carbon sequestration Most of the world's existing energy supply is stored underground in

  12. Lee Berry, Paul Bonoli, David Green

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lee Berry, Paul Bonoli, David Green Lee Berry, Paul Bonoli, David Green FES Requirements Worksheet 1.1. Project Information - Center for Simulation of Wave-Plasma Interactions (aka RF SciDAC) Document Prepared By Lee Berry, Paul Bonoli, David Green Project Title Center for Simulation of Wave-Plasma Interactions (aka RF SciDAC) Principal Investigator Paul Bonoli Participating Organizations Massachusetts Institute of Technology, Princeton Plasma Physics Laboratory, Oak Ridge National Laboratory,

  13. Customer Aggregation: An Opportunity for Green Power?

    SciTech Connect (OSTI)

    Holt, E.; Bird, L.

    2001-02-26

    We undertook research into the experience of aggregation groups to determine whether customer aggregation offers an opportunity to bring green power choices to more customers. The objectives of this report, therefore, are to (1) identify the different types of aggregation that are occurring today, (2) learn whether aggregation offers an opportunity to advance sales of green power, and (3) share these concepts and approaches with potential aggregators and green power advocates.

  14. Covered Product Category: Fluorescent Luminaires | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Luminaires," using IESNA LM-41, ANSI C82.2-2002 for fluorescent ballasts, and ANSI C78.81-2005 for fluorescent lamps. Finding the Luminaire Efficacy Rating LERs should be...

  15. #MySmallAct: 10 Simple Ways to Go Green and Save Green This Earth...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Earth Day MySmallAct: 10 Simple Ways to Go Green and Save Green This Earth Day April 20, 2015 - 4:10pm Addthis Energy Department employee Bill Vandermeer tunes a bicycle as part ...

  16. City of San Francisco- Green Building Code

    Broader source: Energy.gov [DOE]

    San Francisco adopted a mandatory green building code for new construction projects in September 2008, establishing strict guidelines for residential and commercial buildings according to the...

  17. Green Building Standards for State Facilities

    Broader source: Energy.gov [DOE]

    The Rhode Island Department of Administration was required to form a Green Buildings Advisory Committee to support implementation of this policy through education, training, and evaluation recomm...

  18. High Performance Builder Spotlight: Green Coast Enterprises ...

    Energy Savers [EERE]

    Solutions for New Homes: Green Coast Enterprises, New Orleans, Louisiana Building America Best Practices Series Volume 15: 40% Whole-House Energy Savings in the Hot-Humid ...

  19. Green River Biodiesel Incorporated | Open Energy Information

    Open Energy Info (EERE)

    River Biodiesel Incorporated Jump to: navigation, search Name: Green River Biodiesel Incorporated Place: Houston, Texas Zip: 77056 Product: Biodiesel project developer and...

  20. Delaware Municipal Electric Corporation- Green Energy Fund

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Delaware Green Energy Fund was created in 1999 as the part of the deregulation of Delaware's electric utilities. Under the 2005 Delaware renewable portfolio standard (RPS) legislation,...

  1. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications Home > Features > Groups Content Group Activity By term Q & A Feeds Content type Blog entry Discussion Document Event Poll Question Keywords Author...

  2. ITEP Greening Tribal Operations and Facilities Trainings

    Broader source: Energy.gov [DOE]

    The Institute for Tribal Environmental Professionals (ITEP) is offering our Greening Tribal Operations and Facilities training course for employees of federally-recognized tribes.

  3. Trends in Utility Green Pricing Programs (2005)

    Office of Energy Efficiency and Renewable Energy (EERE)

    This report presents year-end 2005 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs. It is important to note that this report covers only a portion of voluntary markets for renewable energy. It does not cover green power sold by independent marketers except for cases in which the marketers work in conjunction with utilities or default electricity suppliers.

  4. Green Pacific Biologicals Inc | Open Energy Information

    Open Energy Info (EERE)

    California Product: A California-based company that has a patent on a technology for nuclear-genetic engineering of algae for biofuel production. References: Green Pacific...

  5. GreenTower | Open Energy Information

    Open Energy Info (EERE)

    Sector: Solar Product: Developer of a solar chimney technology, with greenhouses for food production. Hopes to deploy this in Namibia. References: GreenTower1 This article...

  6. GreenPower International | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: GreenPower International Place: Alloa, Scotland, United Kingdom Zip: FK10 3LP Sector: Hydro, Renewable Energy, Wind energy Product:...

  7. Robert G. Green (Acting) | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Mr. Green also served as the (Acting) Associate Chief Information Officer for Energy IT Services (EITS) and Deputy Associate Chief Information Officer for EITS, where through ...

  8. Delmarva Power- Green Energy Program Incentives

    Office of Energy Efficiency and Renewable Energy (EERE)

    NOTE: Effective September 21, 2015 the Green Energy Program incentives have been updated. Please visit the weblink to access the summary of current incentives. 

  9. NY Green Bank | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    sector to address and alleviate market and financial barriers preventing a thriving clean energy marketplace. NY Green Bank does not accept deposits or offer retail loans, and...

  10. Green Money Journal | Open Energy Information

    Open Energy Info (EERE)

    Money Journal Jump to: navigation, search Name: Green Money Journal Place: Santa Fe, New Mexico Zip: 87504 Sector: Renewable Energy Product: Online magazine covering the financial...

  11. TVA - Green Power Providers | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    from the following qualifying resources: PV, wind, hydropower, and biomass. The long term Green Power Providers program replaces the Generation Partners* pilot program. The energy...

  12. Green Economy Toolbox | Open Energy Information

    Open Energy Info (EERE)

    Economy Toolbox Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Green Economy Toolbox AgencyCompany Organization: United Nations Economic Commission for Europe Sector:...

  13. The Green Grid | Open Energy Information

    Open Energy Info (EERE)

    Grid Jump to: navigation, search Name: The Green Grid Place: Oregon Zip: 97006 Sector: Efficiency Product: Oregan-based consortium that seeks to improve energy efficiency in data...

  14. Green Kiosk Viewer | Open Energy Information

    Open Energy Info (EERE)

    Kiosk Viewer Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Green Kiosk Viewer AgencyCompany Organization: Johnson Controls Sector: Energy User Interface: Website,...

  15. Green Building Studio | Open Energy Information

    Open Energy Info (EERE)

    Studio Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Green Building Studio AgencyCompany Organization: Autodesk Sector: Energy Focus Area: Buildings, Energy...

  16. Green Electronics Council | Open Energy Information

    Open Energy Info (EERE)

    Electronics Council Jump to: navigation, search Name: Green Electronics Council Place: Portland, Oregon Product: Oregon-based program that supports the design, manufacture, use and...

  17. Green Power Conferences | Open Energy Information

    Open Energy Info (EERE)

    Conferences Jump to: navigation, search Name: Green Power Conferences Place: London, Greater London, United Kingdom Zip: SE1 7SJ Sector: Renewable Energy Product: London-based...

  18. Green Isle Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    Isle Energy LLC Jump to: navigation, search Name: Green Isle Energy, LLC Place: Hawaii Zip: 96744 Sector: Renewable Energy Product: Hawaiian company that monitor the performance of...

  19. Green Spirit Fuels | Open Energy Information

    Open Energy Info (EERE)

    Spirit Fuels Jump to: navigation, search Name: Green Spirit Fuels Place: Somerset, United Kingdom Zip: BA8 OTN Sector: Biofuels Product: The company was founded to produce and...

  20. DEMEC - Green Energy Fund | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    PagesGEP%20DE... State Delaware Program Type Public Benefits Fund Summary Note: The Green Energy Fund regulations are currently under revision to improve program function and...

  1. Green Energy Group | Open Energy Information

    Open Energy Info (EERE)

    Group Jump to: navigation, search Name: Green Energy Group Place: Hannover, Lower Saxony, Germany Zip: 30559 Sector: Geothermal energy Product: Holding company which plans to...

  2. Green Gas International | Open Energy Information

    Open Energy Info (EERE)

    International Jump to: navigation, search Name: Green Gas International Place: Richmond upon Thames, United Kingdom Zip: TW10 6UW Product: The company establishes coal mine,...

  3. Green Energy Technologies LLC | Open Energy Information

    Open Energy Info (EERE)

    Technologies LLC Jump to: navigation, search Name: Green Energy Technologies LLC Place: Akron, Ohio Zip: 44333 Sector: Wind energy Product: Ohio-based micro-scale wind power...

  4. Green Energy Ohio | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Ohio Address: 7870 Olentangy River Road, Suite 209 Place: Columbus, Ohio Zip: 43235 Sector: Biomass, Solar, Wind energy Product: Trainining and education Phone Number:...

  5. Green Light Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    LLC Jump to: navigation, search Name: Green Light Energy LLC Place: Reading, Pennsylvania Product: Reading-based energy management consultants. Coordinates: 43.45529,...

  6. Green Solar Finance | Open Energy Information

    Open Energy Info (EERE)

    Solar Finance Jump to: navigation, search Name: Green Solar Finance Place: Connecticut Sector: Solar Product: Connecticut-based arranger of financing for clients of solar project...

  7. Green Design Systems | Open Energy Information

    Open Energy Info (EERE)

    Systems Jump to: navigation, search Name: Green Design Systems Address: PO Box 1229 Place: Healdsburg, California Zip: 95448 Region: Bay Area Sector: Buildings Product: Refuse...

  8. Enel Green Finpiemonte JV | Open Energy Information

    Open Energy Info (EERE)

    Finpiemonte JV Jump to: navigation, search Name: Enel Green & Finpiemonte JV Place: Italy Sector: Renewable Energy Product: Italy-based JV to develop renewable energy resources....

  9. Green, Ohio: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Green, Ohio: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 40.9458898, -81.4831714 Show Map Loading map... "minzoom":false,"mappingservice":"...

  10. Brasil Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Energy Jump to: navigation, search Name: Brasil Green Energy Place: Brazil Sector: Renewable Energy Product: Brazilian-based company which seeks renewable energy investment...

  11. California Green Designs | Open Energy Information

    Open Energy Info (EERE)

    Designs Jump to: navigation, search Name: California Green Designs Place: Encino, California Zip: 91316 Sector: Buildings, Renewable Energy Product: Designs and builds...

  12. Green Ventures Search | Open Energy Information

    Open Energy Info (EERE)

    Search Jump to: navigation, search Name: Green Ventures Search Place: Surrey, United Kingdom Zip: GU3 3BP Sector: Renewable Energy Product: UK-based executive search firm...

  13. Green Energy Capital Partners | Open Energy Information

    Open Energy Info (EERE)

    Capital Partners Jump to: navigation, search Name: Green Energy Capital Partners Place: Plymouth Meeting, Pennsylvania Zip: 19462 Sector: Wind energy Product: Pennsylvania-based...

  14. Green Systems UK | Open Energy Information

    Open Energy Info (EERE)

    Systems UK Jump to: navigation, search Name: Green Systems UK Place: Cambridge, United Kingdom Sector: Solar Product: Design and installation of vacuum tube & flat plate solar hot...

  15. Green Ocean Wave Energy | Open Energy Information

    Open Energy Info (EERE)

    Ocean Wave Energy Jump to: navigation, search Name: Green Ocean Wave Energy Region: United States Sector: Marine and Hydrokinetic Website: http: This company is listed in the...

  16. Shaanxi Green Energy | Open Energy Information

    Open Energy Info (EERE)

    Energy Jump to: navigation, search Name: Shaanxi Green Energy Place: Xianyang, Shanxi Province, China Sector: Geothermal energy Product: A project SPV company which operates a...

  17. Global Green Partners | Open Energy Information

    Open Energy Info (EERE)

    Partners Jump to: navigation, search Name: Global Green Partners Place: Los Altos, California Zip: 94024 Sector: Carbon Product: California-based investment fund prioritizing trade...

  18. Green Cat Renewables | Open Energy Information

    Open Energy Info (EERE)

    Cat Renewables Jump to: navigation, search Name: Green Cat Renewables Region: Scotland Sector: Marine and Hydrokinetic Website: http: This company is listed in the Marine and...

  19. Green Spark Ventures LLC | Open Energy Information

    Open Energy Info (EERE)

    Spark Ventures LLC Jump to: navigation, search Name: Green Spark Ventures, LLC Place: Denver, Colorado Zip: 80203 Sector: Efficiency, Renewable Energy Product: Denver-based venture...

  20. Green Energy Industries Inc | Open Energy Information

    Open Energy Info (EERE)

    Industries Inc Jump to: navigation, search Name: Green Energy Industries Inc Region: United States Sector: Marine and Hydrokinetic Website: http: This company is listed in the...

  1. Green Infrastructure Bonds | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    allowing the Department of Business, Economic Development, and Tourism to issue Green Infrastructure Bonds to secture low-cost financing for clean energy installations,...

  2. EHN Green Energy Developments | Open Energy Information

    Open Energy Info (EERE)

    Developments Jump to: navigation, search Name: EHN Green Energy Developments Place: Spain Sector: Renewable Energy Product: Renewable energy development company, focusing on...

  3. Green Source Consulting | Open Energy Information

    Open Energy Info (EERE)

    Consulting Jump to: navigation, search Name: Green Source Consulting Place: Wien Vienna, Austria Zip: 1010 Product: Private Austrian project developer with a focus in the Central...

  4. Green St Energy | Open Energy Information

    Open Energy Info (EERE)

    Green St. Energy Place: Tehachapi, California Zip: 93561 Sector: Wind energy Product: California-based OTC-quoted wind energy project developer. Coordinates: 35.132245,...

  5. Green Energy Technologies | Open Energy Information

    Open Energy Info (EERE)

    Green Energy Technologies Address: 846 N. Cleveland Massilon Rd. Place: Akron, Ohio Zip: 44333 Sector: Wind energy Product: String representation "Agriculture;Bus ... acturer in...

  6. Green Partners LLC | Open Energy Information

    Open Energy Info (EERE)

    Partners LLC Jump to: navigation, search Name: Green Partners LLC Place: New York Zip: NY 10022 Sector: Efficiency, Renewable Energy Product: US-based investment firm focused on...

  7. Green Earth Fuels | Open Energy Information

    Open Energy Info (EERE)

    Earth Fuels Jump to: navigation, search Name: Green Earth Fuels Place: Houston, Texas Zip: 77057 Product: A producer and distributor of soy and palm based biodiesel Coordinates:...

  8. Green Strategies Inc | Open Energy Information

    Open Energy Info (EERE)

    a business and public policy consulting firm specializing in domestic and international energy and environmental matters. References: Green Strategies Inc1 This article is a...

  9. Green Fuel Technologies Corporation | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: Green Fuel Technologies Corporation Address: 29 Smith Place Place: Cambridge, Massachusetts Zip: 02138 Region: Greater Boston Area Sector:...

  10. Greening Your Job: Careers in Energy

    Broader source: Energy.gov [DOE]

    Recently a friend considering switching careers asked me about job opportunities in renewables and efficiency—often referred to as green jobs.

  11. ITEP Course: Greening Tribal Operations and Facilities

    Broader source: Energy.gov [DOE]

    The Institute for Tribal Environmental Professionals will be offering a new course, Greening Tribal Operations and Facilities in San Diego, California, December 9 -11, 2014, for employees of...

  12. Free Green Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    LLC Jump to: navigation, search Name: Free Green Energy LLC Place: Houston, Texas Zip: 77060 Sector: Geothermal energy Product: Houston-based company formed to develop geothermal...

  13. Green Power Group Ltd | Open Energy Information

    Open Energy Info (EERE)

    Group Ltd Jump to: navigation, search Name: Green Power Group Ltd Sector: Solar Product: A company under Nixon International Group specilized in solar technology R&D. References:...

  14. TVA - Green Power Providers | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    the following qualifying resources: PV, wind, hydropower, and biomass. The long term Green Power Providers program replaces the Generation Partners* pilot program. The energy...

  15. Ramblewood Green: Proposed Penalty (2014-CE-23017)

    Broader source: Energy.gov [DOE]

    DOE alleged in a Notice of Proposed Civil Penalty that Ramblewood Green Ltd. failed to certify cooking products as compliant with the applicable energy conservation standards.

  16. Green Fuel Corporacion | Open Energy Information

    Open Energy Info (EERE)

    Corporacion Jump to: navigation, search Name: Green Fuel Corporacion Place: Spain Product: Plans to develop several biodiesel production plants in the 2005-2007 period, each with...

  17. Green Purchasing under DOE Architect Engineer Contracts

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This clause should be used in any architect-engineer type contract unless Leadership in Energy and Environmental Design (LEED) Green Building Certification is being pursued. In...

  18. Anaheim Public Utilities- Green Building Rebate Program

    Broader source: Energy.gov [DOE]

    Anaheim Public Utilities (APU) offers commercial, industrial, residential, and institutional customers the Green Building Incentives Program to offset construction, installation and upgrade costs...

  19. Green Button Initiative | OpenEI Community

    Open Energy Info (EERE)

    Apps - energy providers are giving customers easy and secure online access to their personal energy use data. The Green Button program offers unique differences and improvements...

  20. China s Green Beat | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: China's Green Beat Place: Beijing, Beijing Municipality, China Zip: 100088 Product: The body create films and podcasts, with a view to promoting...

  1. Green Heat Solutions Limited | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: Green Heat Solutions Limited Region: Scotland Sector: Marine and Hydrokinetic Website: http: This company is listed in the Marine and Hydrokinetic...

  2. Green Mountain Power Corp | Open Energy Information

    Open Energy Info (EERE)

    from Town of Readsboro, Vermont (Utility Company)) Jump to: navigation, search Name: Green Mountain Power Corp Place: Vermont Service Territory: Vermont Phone Number:...

  3. Green Star Alternative Energy | Open Energy Information

    Open Energy Info (EERE)

    Alternative Energy Jump to: navigation, search Name: Green Star Alternative Energy Place: San Diego, California Zip: 92108 Sector: Wind energy Product: A US-based wind energy...

  4. Green Star Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    LLC Jump to: navigation, search Name: Green Star Energy LLC Place: Houston, Texas Zip: 77002 Product: Houston-based producer of sugar cane processed ethanol, with additional...

  5. Green Power Network Newsletter | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    opportunities, publications, and other analyses related to green power markets. Partner Agency: U.S. Department of Energy Resource Type: Newsletter Stakeholder Group(s): ...

  6. USPS: Lean Green Teams | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Connecting Sustainability to the Agency's Mission Driving Operational Changes Through an Energy Monitoring System USPS: Lean Green Teams Enabling Sustainable Acquisition by ...

  7. Robert G. Green | Department of Energy

    Office of Environmental Management (EM)

    Mr. Green also served as the (Acting) Associate Chief Information Officer for Energy IT Services (EITS) and Deputy Associate Chief Information Officer for EITS, where through ...

  8. Vehicular Thermoelectrics: A New Green Technology | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    (8.06 MB) More Documents & Publications Thermoelectrics: The New Green Automotive Technology Automotive Thermoelectric Generators and HVAC Solid-State Energy Conversion Overview

  9. Green Codes and Programs | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    This webinar covers green building codes, standards, and programs. Transcript Presentation (1.87 MB) More Documents & Publications Energy Code Compliance and Enforcement Best ...

  10. Green Purchasing under DOE Architect Engineer Contracts

    Broader source: Energy.gov (indexed) [DOE]

    Use: This clause should be used in any architect-engineer type contract unless Leadership in Energy and Environmental Design (LEED) Green Building Certification is being pursued. ...

  11. New Lab facility receives green building recognition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    All Issues submit New Lab facility receives green building recognition The Radiological Laboratory Utility Office Building is the first to achieve Leadership in Energy and ...

  12. David Crockett, Chattanooga's Green Frontiersman | Department...

    Broader source: Energy.gov (indexed) [DOE]

    to green infrastructure. The city of Chattanooga created the sustainability director position through funds awarded from the U.S. Department of Energy's Energy Efficiency ...

  13. DOE_Sustainability_and_Green_Zia_Awards

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stewardship and for reducing energy, water and paper usage at the DOE's Waste ... CBFO for a Bronze Award under their Green Zia Environmental Leadership Program, ...

  14. Greening the Grid: The Role of Storage and Demand Response, Greening the Grid (Fact Sheet)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Greening Up the Sports World Greening Up the Sports World July 19, 2012 - 2:45pm Addthis By installing a rooftap solar array at Safeco Field, the Seattle Mariners is reducing its carbon footprint and saving money. | Photo courtesy of the Green Sports Alliance. By installing a rooftap solar array at Safeco Field, the Seattle Mariners is reducing its carbon footprint and saving money. | Photo courtesy of the Green Sports Alliance. Arah Schuur Building Technologies Program, Office of Energy

  15. Photoswitchable Red Fluorescent Protein with a Large Stokes Shift...

    Office of Scientific and Technical Information (OSTI)

    Authors: Piatkevich, Kiryl D. ; English, Brian P. ; Malashkevich, Vladimir N. ; Xiao, Hui ... Country of Publication: United States Language: ENGLISH Word Cloud More Like This Full ...

  16. Mapping protein collapse with single molecule fluorescence and...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Proceedings of the National Academy of Sciences of the United States of America, na, na, December 21, 2006, pp. 105 ...

  17. Fluorescent optical liquid level sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D. (Albuquerque, NM)

    2001-01-01

    A liquid level sensor comprising a transparent waveguide containing fluorescent material that is excited by light of a first wavelength and emits at a second, longer wavelength. The upper end of the waveguide is connected to a light source at the first wavelength through a beveled portion of the waveguide such that the input light is totally internally reflected within the waveguide above an air/liquid interface in a tank but is transmitted into the liquid below this interface. Light is emitted from the fluorescent material only in those portions of the waveguide that are above the air/liquid interface, to be collected at the upper end of the waveguide by a detector that is sensitive only to the second wavelength. As the interface moves down in the tank, the signal strength from the detector will increase.

  18. Characterization of a new photo-fluorescent film dosimeter for high-radiation dose applications

    SciTech Connect (OSTI)

    Murphy, Mark K. ); Miller, Steven D. ); Kovacs, Andras; Mclaughlin, William L.; Slezsak, Istvan

    2001-12-01

    Characterization studies on one of the first versions of the Sunna fluorescent dosimeter have been published by Kovacs and McLaughlin. This present study describes testing results of a newer version of the dosimeter (Model and 61543;, batch 0399-20). This dosimeter is a 1-cm by 3-cm polymeric film of 0.5 mm thickness that emits a green fluorescence component at intensities almost linear with dose. The manufacturing method (injection molding) allows potential batch sizes on the order of a million while maintaining a signal precision on the order of+/- 1%. Studies include dose response, dose rate dependence, energy dependence, post-irradiation stability, environmental effects, and variation of response within a batch. Data for both food irradiation and sterilization dose levels were obtained. The results indicate that the green signal (0.3-200 kGy) works well for food irradiation dose levels, especially in refrigerated facilities that maintain tight temperature control. The green signal also works well in sterilization facilities because its irradiation temperature coefficient above room temperature is minimal at sterilization doses. If the user requires readout results in less than 22 hours after room temperature irradiation, the user can either calibrate for a specific post-irradiation readout time(s) or simply heat the dosimeters in a small laboratory oven to quickly stabilize the signal.

  19. Solving the 'Green Gap' in LED Technology

    Broader source: Energy.gov [DOE]

    One long-standing high-priority research area for DOE is to increase the efficiency of deep green LEDs. Although most products today use phosphor conversion (PC) to produce white light from a blue LED, having a good green source could lead to color-mixed white sources that would avoid the losses associated with the PC approach.

  20. Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells

    SciTech Connect (OSTI)

    Chen, Fanqing; Gerion, Daniele

    2004-06-14

    One of the biggest challenges in cell biology is the imaging of living cells. For this purpose, the most commonly used visualization tool is fluorescent markers. However, conventional labels, such as organic fluorescent dyes or green fluorescent proteins (GFP), lack the photostability to allow the tracking of cellular events that happen over minutes to days. In addition, they are either toxic to cells (dyes), or difficult to construct and manipulate (GFP). We report here the use of a new class of fluorescent labels, silanized CdSe/ZnS nanocrystal-peptide conjugates, for imaging the nuclei of living cells. CdSe/ZnS nanocrystals, or so called quantum dots (qdots), are extremely photostable, and have been used extensively in cellular imaging of fixed cells. However, most of the studies about living cells so far have been concerned only with particle entry into the cytoplasm or the localization of receptors on the cell membrane. Specific targeting of qdots to the nucleus of living cells ha s not been reported in previous studies, due to the lack of a targeting mechanism and proper particle size. Here we demonstrate for the first time the construction of a CdSe/ZnS nanocrystal-peptide conjugate that carries the SV40 large T antigen nuclear localization signal (NLS), and the transfection of the complex into living cells. By a novel adaptation of commonly used cell transfection techniques for qdots, we were able to introduce and retain the NLS-qdots conjugate in living cells for up to a week without detectable negative cellular effects. Moreover, we can visualize the movement of the CdSe/ZnS nanocrystal-peptide conjugates from cytoplasm to the nucleus, and the accumulation of the complex in the cell nucleus, over a long observation time period. This report opens the door for using qdots to visualize long-term biological events that happen in the cell nucleus, and provides a new nontoxic, long-term imaging platform for cell nuclear processes.

  1. Fluorescence based chemical sensors for corrosion detection

    SciTech Connect (OSTI)

    Johnson, R.E.; Agarwala, V.S.

    1997-12-01

    Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.

  2. Property:GreenButtonWebpage | Open Energy Information

    Open Energy Info (EERE)

    GreenButtonWebpage Jump to: navigation, search Property Name GreenButtonWebpage Property Type URL Description This field is the public landing page for Green Button data on the...

  3. Blue Sky Green Field Wind Farm | Open Energy Information

    Open Energy Info (EERE)

    Green Field Wind Farm Jump to: navigation, search Name Blue Sky Green Field Wind Farm Facility Blue Sky Green Field Wind Farm Sector Wind energy Facility Type Commercial Scale Wind...

  4. U.S. Energy Information Administration | Green Pricing and Net...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    U.S. Energy Information Administration | Green Pricing and Net Metering Programs 2009 Green Pricing and Net Metering Programs 2009 Table 5.1 Estimated U.S. Green Pricing Customers ...

  5. Taking It from Brown to Green: Renewable Energy on Contaminated...

    Broader source: Energy.gov (indexed) [DOE]

    Taking It from Brown to Green: Renewable Energy on Contaminated Lands - Otto Van Geet (2.61 MB) Brown to Green: Brownfields Redevelopment for Green Power - Johnson (4.79 MB) ...

  6. GREEN GALAXIES IN THE COSMOS FIELD

    SciTech Connect (OSTI)

    Pan, Zhizheng; Kong, Xu; Fan, Lulu E-mail: xkong@ustc.edu.cn

    2013-10-10

    We present research on the morphologies, spectra, and environments of ≈2350 'green valley' galaxies at 0.2 < z < 1.0 in the COSMOS field. The bimodality of dust-corrected NUV–r {sup +} color is used to define 'green valley'; it removes dusty star-forming galaxies from galaxies that are truly transitioning between the blue cloud and the red sequence. Morphological parameters of green galaxies are intermediate between those of blue and red galaxy populations, both on the Gini-asymmetry and the Gini-M{sub 20} planes. Approximately 60%-70% of green disk galaxies have intermediate or big bulges, and only 5%-10% are pure disk systems, based on morphological classification using the Zurich Estimator of Structural Types. The obtained average spectra of green galaxies are intermediate between blue and red ones in terms of [O II], Hα, and Hβ emission lines. Stellar population synthesis on the average spectra shows that green galaxies are on average older than blue galaxies but younger than red galaxies. Green galaxies and blue galaxies have similar projected galaxy density (Σ{sub 10}) distributions at z > 0.7. At z < 0.7, the fractions of M{sub *} < 10{sup 10.0} M{sub ☉} green galaxies located in a dense environment are found to be significantly larger than those of blue galaxies. The morphological and spectral properties of green galaxies are consistent with the transitioning population between the blue cloud and the red sequence. The possible mechanisms for quenching star formation activities in green galaxies are discussed. The importance of active galactic nucleus feedback cannot be well constrained in our study. Finally, our findings suggest that environmental conditions, most likely starvation and harassment, significantly affect the transformation of M{sub *} < 10{sup 10.0} M{sub ☉} blue galaxies into red galaxies, especially at z < 0.5.

  7. Analytical Methods for Malachite Green : Completion Report : Malachite Green Analysis in Water.

    SciTech Connect (OSTI)

    Allen, John L.; Gofus, Jane E.; Meinertz, Jeffery R.

    1991-06-01

    Malachite green is a known teratogen and therefore its use is limited to nonfood fish under an Investigational New Animal Drug permit (INAD), number 2573. Although a charcoal adsorption column was developed to remove malachite green from hatchery water, INAD compliance requires that the malachite green residue concentrations in any effluent from hatcheries using the chemical be quantified. Therefore, we developed a method for the analysis of malachite green residues in water. Enrichment of the residues of malachite green in water on a diol column followed by High Performance Liquid Chromatographic (HPLC) analysis gives a minimum sensitivity of less than 10 ppb for the chemical. When combined with post-column oxidation using a lead oxide post-column reactor, the procedure can be used for the simultaneous analysis of malachite green in its leuco form, a decomposition product of the dye, as well as its chromatic form. Recovery of the leuco form is pH dependent and water samples should be adjusted to pH 6 to optimize recovery of this form. Water samples spiked with malachite green were concentrated on a diol column followed by elution with 0.05 M p-toluene sulfonic acid in methanol. The methanol elutes were analyzed by HPLC. Pond water samples spiked with malachite green and leuco malachite green yielded average recoveries of 95.4% for malachite green and 57.3% for leuco malachite green. Tap water samples spiked with the carbinol form of malachite green gave average recoveries of 98.6%. The method is very sensitive and is capable of detecting malachite green residues in water at less than 10 ppb. Fish culturists, who cannot find an effective replacement for malachite green, can utilize the method to ensure that their effluents comply with INAD regulations. 13 refs., 2 figs., 7 tabs.

  8. Leveraging Portfolio Manager for Disclosure and Green Leasing...

    Energy Savers [EERE]

    Portfolio Manager for Disclosure and Green Leasing Practices Leveraging Portfolio Manager for Disclosure and Green Leasing Practices A talk about developing paths and implementing ...

  9. From Climate Finance to Financing Green Growth | Open Energy...

    Open Energy Info (EERE)

    green growth and the importance of developing the right policies to support a transition towards the low carbon economy. It assesses the financing needs of green growth in...

  10. Commerce Department to Speed Review of Green Technology Patents...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    is designed to speed the development and deployment of green technology, create green jobs, and promote U.S. competitiveness. In particular, the program focuses on...

  11. Greene Valley Gas Recovery Biomass Facility | Open Energy Information

    Open Energy Info (EERE)

    Valley Gas Recovery Biomass Facility Jump to: navigation, search Name Greene Valley Gas Recovery Biomass Facility Facility Greene Valley Gas Recovery Sector Biomass Facility Type...

  12. EECBG Success Story: David Crockett, Chattanooga's Green Frontiersman...

    Energy Savers [EERE]

    David Crockett, Chattanooga's Green Frontiersman EECBG Success Story: David Crockett, Chattanooga's Green Frontiersman June 11, 2010 - 5:15pm Addthis Crockett has seen Chattanooga ...

  13. Renewable Energy Price-Stability Benefits in Utility Green Power...

    Office of Scientific and Technical Information (OSTI)

    Price-Stability Benefits in Utility Green Power Programs. 36 pp Citation Details In-Document Search Title: Renewable Energy Price-Stability Benefits in Utility Green Power ...

  14. Enhancing low-carbon development by greening the economy: policy...

    Open Energy Info (EERE)

    low-carbon development by greening the economy: policy dialogue, advisory services, benchmarking Jump to: navigation, search Name Enhancing low-carbon development by greening the...

  15. 21st century Green Solutions LLC | Open Energy Information

    Open Energy Info (EERE)

    21st century Green Solutions LLC Jump to: navigation, search Name: 21st century Green Solutions, LLC Place: Grand Blanc, Michigan Zip: 48439 Sector: Wind energy Product: Exclusive...

  16. Federal Efficiency Program Wins GreenGov Dream Team Award

    Office of Energy Efficiency and Renewable Energy (EERE)

    Partnered with the Department of Transportation (DOT), EEREs Federal Energy Management Program (FEMP) won the GreenGov Presidential Green Dream Team Award.

  17. Nanjing Green Waste Recovery Engineering Co Ltd | Open Energy...

    Open Energy Info (EERE)

    Nanjing Green Waste Recovery Engineering Co Ltd Jump to: navigation, search Name: Nanjing Green Waste Recovery Engineering Co. Ltd Place: Nanjing, Jiangsu Province, China Zip:...

  18. Green Capital Consulting Company GCCC | Open Energy Information

    Open Energy Info (EERE)

    Consulting Company GCCC Jump to: navigation, search Name: Green Capital Consulting Company (GCCC) Place: Beijing, China Zip: 100022 Product: Green Capital Consulting Company (GCCC)...

  19. MHK Technologies/Deep Green | Open Energy Information

    Open Energy Info (EERE)

    MHK TechnologiesDeep Green < MHK Technologies Jump to: navigation, search << Return to the MHK database homepage Deep Green.jpg Technology Profile Primary Organization Minesto AB...

  20. Attachment 3: Instructions for Use of Green Lease Solicitation...

    Energy Savers [EERE]

    Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart...

  1. Attachment 2: Solicitation for Offers with New and Revised Green...

    Energy Savers [EERE]

    Attachment 2: Solicitation for Offers with New and Revised Green Lease Text Attachment 2: Solicitation for Offers with New and Revised Green Lease Text PDF icon Solicitation for...

  2. Green Lease Policies and Procedures for Lease Acquisition | Department...

    Energy Savers [EERE]

    Green Lease Policies and Procedures for Lease Acquisition Green Lease Policies and Procedures for Lease Acquisition PDF icon RSL-2007-12 More Documents & Publications Attachment 1:...

  3. Guide to Purchasing Green Power: Renewable Electricity, Renewable...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Guide to Purchasing Green Power: Renewable Electricity, Renewable Energy Certificates, and On-Site Renewable Generation Guide to Purchasing Green Power: Renewable Electricity, ...

  4. Iowa: West Union Green Transformation Project | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    West Union Green Transformation Project Iowa: West Union Green Transformation Project ... These grant funds will be used to close up their buildings-making them more energy ...

  5. The Energy Efficiency and Renewable Energy (EERE) blog on Green...

    Open Energy Info (EERE)

    The Energy Efficiency and Renewable Energy (EERE) blog on Green Button is live Home > Groups > Green Button Applications Dc's picture Submitted by Dc(266) Contributor 27 February,...

  6. Sree Rayalseema Green Energy Limited | Open Energy Information

    Open Energy Info (EERE)

    Rayalseema Green Energy Limited Jump to: navigation, search Name: Sree Rayalseema Green Energy Limited Place: Gooty, Andhra Pradesh, India Zip: 515 402 Sector: Biomass Product: A...

  7. Jiawei Solarchina Dynamic Green Energy Ltd | Open Energy Information

    Open Energy Info (EERE)

    Jiawei Solarchina Dynamic Green Energy Ltd Jump to: navigation, search Name: Jiawei Solarchina Dynamic Green Energy Ltd Place: Shenzhen, Guangdong Province, China Zip: 518001...

  8. Bull Frog Green Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    Frog Green Energy LLC Jump to: navigation, search Name: Bull Frog Green Energy LLC Place: Carlsbad, California Zip: 92009 Product: A company, probably an individual, which has...

  9. Qinghai Gofly Green Energy Ltd Co | Open Energy Information

    Open Energy Info (EERE)

    Gofly Green Energy Ltd Co Jump to: navigation, search Name: Qinghai Gofly Green Energy Ltd Co Place: Xining, Qinghai Province, China Product: Chinese PV module maker. Coordinates:...

  10. Sanhe Green Lishu New Energy Development Co Ltd | Open Energy...

    Open Energy Info (EERE)

    Green Lishu New Energy Development Co Ltd Jump to: navigation, search Name: Sanhe Green (Lishu) New Energy Development Co. Ltd. Place: Jilin Province, China Zip: 136520 Sector:...

  11. Thailand-Green Growth Planning | Open Energy Information

    Open Energy Info (EERE)

    search Name Thailand-Green Growth Planning AgencyCompany Organization Global Green Growth Institute (GGGI) Partner Korea International Cooperation Agency (KOICA)...

  12. Spark Green Energy Pvt Ltd | Open Energy Information

    Open Energy Info (EERE)

    Spark Green Energy Pvt Ltd Jump to: navigation, search Name: Spark Green Energy Pvt Ltd. Place: Mumbai, Maharashtra, India Zip: 400 050 Sector: Biomass Product: Mumbai-based...

  13. City of Chicago - Green Permit Program | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    timeline for projects which are designed to maximize indoor air quality and conserve energy and resources. Green Permit Program Incentives Projects accepted into the Green...

  14. Cambodia-Green Growth Planning | Open Energy Information

    Open Energy Info (EERE)

    search Name Cambodia-Green Growth Planning AgencyCompany Organization Global Green Growth Institute (GGGI) Partner Korea International Cooperation Agency (KOICA)...

  15. Green Growth e-Learning | Open Energy Information

    Open Energy Info (EERE)

    Tool Summary LAUNCH TOOL Name: Green Growth e-Learning AgencyCompany Organization: Green Growth Best Practice Initiative (GGBPI) Focus Area: Economic Development, Energy...

  16. Colorado Green Wind Power Project | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name Colorado Green Wind Power Project Facility Colorado Green Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  17. Wuxi Guofei Green Energy Source Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    Guofei Green Energy Source Co Ltd Jump to: navigation, search Name: Wuxi Guofei Green Energy Source Co Ltd Place: Wuxi, Jiangsu Province, China Zip: 214142 Sector: Solar Product:...

  18. Peru-Green Growth Planning | Open Energy Information

    Open Energy Info (EERE)

    navigation, search Name Peru-Green Growth Planning AgencyCompany Organization Global Green Growth Institute (GGGI) Partner Korea International Cooperation Agency (KOICA)...

  19. Huzhou Nantaihu Green Energy Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    Huzhou Nantaihu Green Energy Co Ltd Jump to: navigation, search Name: Huzhou Nantaihu Green Energy Co., Ltd. Place: Huzhou, Zhejiang Province, China Sector: Biomass Product:...

  20. Shanghai Topsolar Green Energy Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    Topsolar Green Energy Co Ltd Jump to: navigation, search Name: Shanghai Topsolar Green Energy Co Ltd Place: Shanghai, Shanghai Municipality, China Zip: 200240 Product: Chinese...

  1. How Would You Rebuild a Town - Green? | Open Energy Information

    Open Energy Info (EERE)

    Rebuild a Town - Green? Jump to: navigation, search Name How Would You Rebuild a Town - Green? AgencyCompany Organization U.S. Department of Energy Partner Greensburg...

  2. Jordan-Green Growth Planning | Open Energy Information

    Open Energy Info (EERE)

    navigation, search Name Jordan-Green Growth Planning AgencyCompany Organization Global Green Growth Institute (GGGI) Partner Korea International Cooperation Agency (KOICA)...

  3. Ethiopia-Green Growth Planning | Open Energy Information

    Open Energy Info (EERE)

    search Name Ethiopia-Green Growth Planning AgencyCompany Organization Global Green Growth Institute (GGGI), Korea International Cooperation Agency (KOICA) Partner...

  4. West Bengal Green Energy Development Corporation WBGEDC | Open...

    Open Energy Info (EERE)

    Bengal Green Energy Development Corporation WBGEDC Jump to: navigation, search Name: West Bengal Green Energy Development Corporation (WBGEDC) Place: Kolkata, West Bengal, India...

  5. Promoting a Green Economy through Clean Transportation Alternatives...

    Broader source: Energy.gov (indexed) [DOE]

    KB) More Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives EV ...

  6. Promoting a Green Economy through Clean Transportation Alternatives...

    Broader source: Energy.gov (indexed) [DOE]

    Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives Town of Hempstead: Project Energy, From ...

  7. Transtech Green Power P Ltd | Open Energy Information

    Open Energy Info (EERE)

    Transtech Green Power P Ltd Jump to: navigation, search Name: Transtech Green Power (P) Ltd Place: Jaipur, Rajasthan, India Zip: 302001 Sector: Biomass, Solar Product:...

  8. City of Pittsburgh Implementation Model: Green Initiatives Trust...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    City of Pittsburgh Implementation Model: Green Initiatives Trust Fund City of Pittsburgh implementation model, Green initiatives trust fund. Author: U. S. Department of Energy City ...

  9. Webtrends Archives by Fiscal Year - Green Power and Greensburg...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Power and Greensburg Webtrends Archives by Fiscal Year - Green Power and Greensburg ... Archives by Fiscal Year - News Webtrends Archives by Fiscal Year - Tribal Energy Program

  10. Green Button Gamer: Driver Challenge | Open Energy Information

    Open Energy Info (EERE)

    Device Website: greenbuttongamer.com Web Application Link: greenbuttongamer.com Cost: Free OpenEI Keyword(s): Challenge Generated Green Button Gamer Screenshot References: Green...

  11. Green Canyon Hot Springs Pool & Spa Low Temperature Geothermal...

    Open Energy Info (EERE)

    Pool & Spa Low Temperature Geothermal Facility Jump to: navigation, search Name Green Canyon Hot Springs Pool & Spa Low Temperature Geothermal Facility Facility Green Canyon Hot...

  12. Green Canyon Hot Springs Greenhouse Low Temperature Geothermal...

    Open Energy Info (EERE)

    Greenhouse Low Temperature Geothermal Facility Jump to: navigation, search Name Green Canyon Hot Springs Greenhouse Low Temperature Geothermal Facility Facility Green Canyon Hot...

  13. ASEM Green Independent Power Producers Network | Open Energy...

    Open Energy Info (EERE)

    ASEM Green Independent Power Producers Network Jump to: navigation, search Name: ASEM Green Independent Power Producers Network Place: Germany Sector: Renewable Energy Product: A...

  14. Green Ridge Power Wind Farm II | Open Energy Information

    Open Energy Info (EERE)

    II Jump to: navigation, search Name Green Ridge Power Wind Farm II Facility Green Ridge Power Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service...

  15. Green Button Applications - Q & A | OpenEI Community

    Open Energy Info (EERE)

    Green Button Applications - Q & A Home > Green Button Applications Content Group Activity By term Q & A Feeds Question Post date Answers Greenbutton datacustodian documentation 2...

  16. Green's function multiple-scattering theory with a truncated...

    Office of Scientific and Technical Information (OSTI)

    Green's function multiple-scattering theory with a truncated basis set: An augmented-KKR formalism Prev Next Title: Green's function multiple-scattering theory with a ...

  17. Better Buildings Workforce Peer Exchange and Green for All High...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Babylon, NY Pam Fendt, Green Jobs Marketing Representative, LIUNA Great Lakes Region ... LIUNA Presenter: Pam Fendt, Green Jobs Marketing Representative, LIUNA Great Lakes Region ...

  18. Beijing LN Green Power Company | Open Energy Information

    Open Energy Info (EERE)

    Beijing LN Green Power Company Jump to: navigation, search Name: Beijing LN Green Power Company Place: Beijing, Beijing Municipality, China Zip: 100000 Sector: Vehicles Product:...

  19. Orient Green Power Company Ltd OGPL | Open Energy Information

    Open Energy Info (EERE)

    Orient Green Power Company Ltd OGPL Jump to: navigation, search Name: Orient Green Power Company Ltd (OGPL) Place: Chennai, Tamil Nadu, India Zip: 600 095 Sector: Biomass, Hydro,...

  20. Indur Green Power Pvt Ltd | Open Energy Information

    Open Energy Info (EERE)

    Indur Green Power Pvt Ltd Jump to: navigation, search Name: Indur Green Power Pvt. Ltd. Place: Hyderabad, Andhra Pradesh, India Zip: 500 029 Sector: Biomass Product:...

  1. Singaraya Hills Green Power Pvt Ltd | Open Energy Information

    Open Energy Info (EERE)

    Singaraya Hills Green Power Pvt Ltd Jump to: navigation, search Name: Singaraya Hills Green Power Pvt. Ltd. Place: Vijayawada, Andhra Pradesh, India Zip: 520 010 Sector: Biomass...

  2. Prayag Green Solar Power PGSP | Open Energy Information

    Open Energy Info (EERE)

    Prayag Green Solar Power PGSP Jump to: navigation, search Name: Prayag Green Solar Power (PGSP) Place: Uttar Pradesh, India Product: Setting up PV project in Uttar Pradesh....

  3. Sri Swarna Green Power SSGP | Open Energy Information

    Open Energy Info (EERE)

    Swarna Green Power SSGP Jump to: navigation, search Name: Sri Swarna Green Power (SSGP) Place: Hyderabad, Andhra Pradesh, India Zip: 500033 Sector: Wind energy Product:...

  4. OECD-Fostering Innovation for Green Growth | Open Energy Information

    Open Energy Info (EERE)

    Fostering Innovation for Green Growth Jump to: navigation, search Tool Summary LAUNCH TOOL Name: OECD-Fostering Innovation for Green Growth AgencyCompany Organization:...

  5. Dyess Air Force Base Water Conservation and Green Energy | Department...

    Energy Savers [EERE]

    Dyess Air Force Base Water Conservation and Green Energy Dyess Air Force Base Water Conservation and Green Energy Fact sheet describes the Federal Energy Management Program (FEMP) ...

  6. Valuing Green in the Appraisal Process - Building America Top...

    Energy Savers [EERE]

    Valuing Green in the Appraisal Process - Building America Top Innovation Real estate appraisers have historically faced challenges with green and energy efficient homes, both in ...

  7. China Power International Shanghai Green CLP JV | Open Energy...

    Open Energy Info (EERE)

    Shanghai Green CLP JV Jump to: navigation, search Name: China Power International, Shanghai Green & CLP JV Place: Shanghai, Shanghai Municipality, China Sector: Wind energy...

  8. China Wind Systems formerly Green Power Malex | Open Energy Informatio...

    Open Energy Info (EERE)

    formerly Green Power Malex Jump to: navigation, search Name: China Wind Systems (formerly Green PowerMalex) Place: Wuxi, Jiangsu Province, China Sector: Wind energy Product:...

  9. American Green Holdings LLC AGH | Open Energy Information

    Open Energy Info (EERE)

    Green Holdings LLC AGH Jump to: navigation, search Name: American Green Holdings LLC (AGH) Place: Montana Product: Montana-based biodiesel manufacturer, using high-pressure...

  10. Beijing Sanyuan Green Lighting Technology Development Co Ltd...

    Open Energy Info (EERE)

    Sanyuan Green Lighting Technology Development Co Ltd Jump to: navigation, search Name: Beijing Sanyuan Green Lighting Technology Development Co., Ltd Place: Beijing, Beijing...

  11. SEP Success Story: Launching Green Entrepreneurship in New Hampshire

    Office of Energy Efficiency and Renewable Energy (EERE)

    This summer, five green companies in New Hampshire are participating in an intensive business accelerator initiative called the Green Launching Pad (GLP). Learn more.

  12. Village of Hazel Green, Wisconsin (Utility Company) | Open Energy...

    Open Energy Info (EERE)

    Green, Wisconsin (Utility Company) Jump to: navigation, search Name: Village of Hazel Green Place: Wisconsin Phone Number: 608.854.2953 Website: villageofhazelgreen.orgabout...

  13. Farming First-Agriculture and the Green Economy | Open Energy...

    Open Energy Info (EERE)

    Farming First-Agriculture and the Green Economy Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Farming First-Agriculture and the Green Economy AgencyCompany...

  14. Green Star Products Inc GSPI | Open Energy Information

    Open Energy Info (EERE)

    Star Products Inc GSPI Jump to: navigation, search Name: Green Star Products Inc (GSPI) Place: Chula Vista, California Zip: 91911 Product: Green Star is building two 200-hectare...

  15. Ghana Goes for Green Growth: National Engagement on Climate Change...

    Open Energy Info (EERE)

    Ghana Goes for Green Growth: National Engagement on Climate Change Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Ghana Goes for Green Growth: National Engagement on...

  16. Korea's Green Growth Strategy: Mitigating Climate Change and...

    Open Energy Info (EERE)

    Korea's Green Growth Strategy: Mitigating Climate Change and Developing New Growth Engines Jump to: navigation, search Name Korea's Green Growth Strategy: Mitigating Climate Change...

  17. U.S. Energy Information Administration | Green Pricing and Net...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Green Pricing and Net Metering Programs 2009 4 Green Pricing and Net Metering Programs ... Source: Energy Information Administration, Form EIA-861, "Annual Electric Power Industry ...

  18. Green Lease Policies and Procedures for Lease Acquisition | Department...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Lease Policies and Procedures for Lease Acquisition Green Lease Policies and Procedures for Lease Acquisition RSL-2007-12 (40.58 KB) More Documents & Publications Attachment ...

  19. Attachment 2: Solicitation for Offers with New and Revised Green...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2: Solicitation for Offers with New and Revised Green Lease Text Attachment 2: Solicitation for Offers with New and Revised Green Lease Text Solicitation for Offers Paragraphs with ...

  20. Los Angeles County's Green Idea House Achieves Efficient Goals...

    Energy Savers [EERE]

    County's Green Idea House Achieves Efficient Goals Los Angeles County's Green Idea House Achieves Efficient Goals Photo of an energy-efficient home with modern architecture. The ...

  1. Green Button Sample from Texas | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    from Texas Green Button Sample from Texas These files contain sample Green Button data ... FY 2014 Funding History Detail Spreadsheet NREL's Clean Energy Policy Analyses Project: ...

  2. Obama Administration Announces $12 Million i6 Green Investment...

    Energy Savers [EERE]

    12 Million i6 Green Investment to Promote Clean Energy Innovation and Job Creation Obama Administration Announces 12 Million i6 Green Investment to Promote Clean Energy ...

  3. Obama Administration Announces Launch of i6 Green Challenge to...

    Office of Environmental Management (EM)

    Obama Administration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth Obama Administration Announces Launch of i6 Green Challenge to ...

  4. Local Energy Alliance Program Adds Green Appraisal Capabilities...

    Energy Savers [EERE]

    Local Energy Alliance Program Adds Green Appraisal Capabilities to its Energy Efficiency Services Local Energy Alliance Program Adds Green Appraisal Capabilities to its Energy ...

  5. Middle School Students Go Green | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    field, green roof, green building products, daylight harvesting system, solar panels and rainwater catchment system. Through the Kentucky Department for Energy Development ...

  6. BETO Partners Win 20th Annual Presidential Green Chemistry Challenge...

    Broader source: Energy.gov (indexed) [DOE]

    Two of the six winners of the 20th Annual Presidential Green Chemistry Challenge ... technologies that promote environmental and economic benefits using green chemistry. ...

  7. Attachment 3: Instructions for Use of Green Lease Solicitation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart (1.84 MB) ...

  8. Updated Section H Greening Clauses | Department of Energy

    Office of Environmental Management (EM)

    Updated Section H Greening Clauses We are updating the Section H Green clauses. The intent ... Sample Contract Language for Construction Using Energy-Efficient Products Updated ...

  9. Attachment 1: Green Lease Policies and Procedures for Lease Acquisitio...

    Broader source: Energy.gov (indexed) [DOE]

    Green Lease Policies and Procedures for Lease Acquisition Attachment 2: Solicitation for Offers with New and Revised Green Lease Text Energy Efficiency in Separate Tenant Spaces - ...

  10. Austin Using Green Innovation to Beat the Utility Blues | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Using Green Innovation to Beat the Utility Blues Austin Using Green Innovation to Beat the ... the treatment processes into clean energy that can power public infrastructure facilities. ...

  11. Carolina Blue Skies & Green Jobs Initiative | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    More Documents & Publications Carolinas Blue Skies & Green Jobs Initiative Carolina Blue Skies & Green Jobs Initiative New York State-wide Alternative Fuel Vehicle Program for ...

  12. Green Button Data: More Power to You | Department of Energy

    Energy Savers [EERE]

    Christopher Irwin Program Analyst, Office of Electricity Delivery and Energy Reliability What is the Green Button initiative? Green Button provides millions of utility customers ...

  13. NREL Highlights Utility Green Power Leaders - News Releases ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wind is the primary source of electricity generated for green energy programs nationwide. "Despite the economic downturn, utility green power sales continued to expand nationally ...

  14. 3M COMPANY SEP PRESENTATION AT 2015 EMERGING GREEN | Department...

    Energy Savers [EERE]

    Technical Assistance Superior Energy Performance 3M COMPANY SEP PRESENTATION AT 2015 EMERGING GREEN 3M COMPANY SEP PRESENTATION AT 2015 EMERGING GREEN seplogoborderless.jpg ...

  15. WIPP Receives Green Zia Award for Environmental Excellence |...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Zia Award for Environmental Excellence WIPP Receives Green Zia Award for ... Plant (WIPP), the U.S. Department of Energy (DOE) facility known as the nation's only ...

  16. Applicability of a Hybrid Retorting Technology in the Green River...

    Broader source: Energy.gov (indexed) [DOE]

    in the Green River Formation that is amenable to commercial development using such hybrid technology. Applicability of a Hybrid Retorting Technology in the Green River ...

  17. Carolina Blue Skies & Green Jobs Initiative | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    (577.38 KB) More Documents & Publications Carolinas Blue Skies & Green Jobs Initiative Carolina Blue Skies & Green Jobs Initiative Advanced Electric Drive Vehicle Education Program

  18. Community Power Works: Good Jobs, Green Jobs Conference | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Works: Good Jobs, Green Jobs Conference Community Power Works: Good Jobs, Green Jobs ... to Extend Program Reach Contractor Quality Assurance and Home Performance with Energy Star

  19. Raman scattering and red fluorescence in the photochemical transformation of dry tryptophan particles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lai, Chih Wei; Schwab, Mark; Hill, Steven C.; Santarpia, Joshua; Pan, Yong -Le

    2016-05-19

    Tryptophan is a fluorescent amino acid common in proteins. Its absorption is largest for wavelengths λ ≲ 290 nm and its fluorescence emissions peak around 300–350 nm, depending upon the local environment. Here we report the observation of red fluorescence near 600 nm emerging from 488-nm continuous-wave (CW) laser photoexcitation of dry tryptophan (Trp) particles. With an excitation intensity below 0.5 kW/cm2, dry Trp particles yield distinctive Raman scattering peaks in the presence of relatively weak and spectrally broad emissions with λ ~500–700 nm, allowing estimation of particle temperature at low excitation intensities. When the photoexcitation intensity is increased tomore » 1 kW/cm2 or more for a few minutes, fluorescence intensity dramatically increases by more than two orders of magnitude. The fluorescence continues to increase in intensity and gradually shift to the red when photoexcitation intensity and the duration of exposure are increased. The resulting products absorb at visible wavelengths and generate red fluorescence with λ ~ 650–800 nm with 633-nm CW laser excitation. In conclusion, we attribute the emergence of orange and red fluorescence in the Trp products to a photochemical transformation that is instigated by weak optical transitions to triplet states in Trp with 488-nm excitation and which may be expedited by a photothermal effect.« less

  20. Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri

    SciTech Connect (OSTI)

    Prochnik, Simon E.; Umen, James; Nedelcu, Aurora; Hallmann, Armin; Miller, Stephen M.; Nishii, Ichiro; Ferris, Patrick; Kuo, Alan; Mitros, Therese; Fritz-Laylin, Lillian K.; Hellsten, Uffe; Chapman, Jarrod; Simakov, Oleg; Rensing, Stefan A.; Terry, Astrid; Pangilinan, Jasmyn; Kapitonov, Vladimir; Jurka, Jerzy; Salamov, Asaf; Shapiro, Harris; Schmutz, Jeremy; Grimwood, Jane; Lindquist, Erika; Lucas, Susan; Grigoriev, Igor V.; Schmitt, Rudiger; Kirk, David; Rokhsar, Daniel S.

    2010-07-01

    Analysis of the Volvox carteri genome reveals that this green alga's increased organismal complexity and multicellularity are associated with modifications in protein families shared with its unicellular ancestor, and not with large-scale innovations in protein coding capacity. The multicellular green alga Volvox carteri and its morphologically diverse close relatives (the volvocine algae) are uniquely suited for investigating the evolution of multicellularity and development. We sequenced the 138 Mb genome of V. carteri and compared its {approx}14,500 predicted proteins to those of its unicellular relative, Chlamydomonas reinhardtii. Despite fundamental differences in organismal complexity and life history, the two species have similar protein-coding potentials, and few species-specific protein-coding gene predictions. Interestingly, volvocine algal-specific proteins are enriched in Volvox, including those associated with an expanded and highly compartmentalized extracellular matrix. Our analysis shows that increases in organismal complexity can be associated with modifications of lineage-specific proteins rather than large-scale invention of protein-coding capacity.