National Library of Energy BETA

Sample records for green fluorescent protein

  1. Mutations that suppress the thermosensitivity of green fluorescent protein

    E-Print Network [OSTI]

    Haseloff, Jim

    Mutations that suppress the thermosensitivity of green fluorescent protein Kirby R. Siemering*, Ralph Golbik, Richard Sever* and Jim Haseloff* Background: The green fluorescent protein (GFP temperatures. Background The green fluorescent protein (GFP) from the bio- luminescent jellyfish Aequorea

  2. Imaging green fluorescent protein in transgenic plants. Jim Haseloff

    E-Print Network [OSTI]

    Haseloff, Jim

    light, and an accessory green fluorescent protein (GFP), which accepts energy from aequorin and reImaging green fluorescent protein in transgenic plants. Jim Haseloff MRC Laboratory of Molecular of seedlings. The green fluorescent protein (GFP) from the cnidarian jellyfish Aequorea victoria shares none

  3. Engineering and Characterization of a Superfolder Green Fluorescent Protein

    SciTech Connect (OSTI)

    Pedelacq,J.; Cabantous, S.; Tran, T.; Terwilliger, T.; Waldo, G.

    2006-01-01

    Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

  4. Methods in Molecular Biology: Protocols in Confocal microscopy Chapter 17. Live imaging of green fluorescent protein

    E-Print Network [OSTI]

    Haseloff, Jim

    , an accessory protein that accepts energy from aequorin and re-emits it as green light [8] . GFP is an extremelyMethods in Molecular Biology: Protocols in Confocal microscopy Chapter 17. Live imaging of green biologists won the lottery when it was demonstrated that green fluorescent protein (GFP), a kind gift from

  5. Split green fluorescent protein as a modular binding partner for protein crystallization

    SciTech Connect (OSTI)

    Nguyen, Hau B. [Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States); Hung, Li-Wei [Los Alamos National Laboratory, MS D454, Los Alamos, NM 87545 (United States); Yeates, Todd O. [University of California, PO Box 951569, Los Angeles, CA 90095 (United States); Terwilliger, Thomas C., E-mail: terwilliger@lanl.gov; Waldo, Geoffrey S., E-mail: terwilliger@lanl.gov [Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States)

    2013-12-01

    A strategy using a new split green fluorescent protein (GFP) as a modular binding partner to form stable protein complexes with a target protein is presented. The modular split GFP may open the way to rapidly creating crystallization variants. A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP ?-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10–11) hairpin in complex with GFP(1–9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10–11) hairpin with a variety of GFP(1–9) mutants engineered for favorable crystallization.

  6. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons 

    E-Print Network [OSTI]

    Zhao, Suling; Maxwell, Sarah L; Jimenez-Beristain, Antonio; Vives, Joaquim; Kuehner, Eva; Zhao, Jiexin; O'Brian, Carmel; de Felipe, Carmen; Semina, Elena

    2004-01-01

    We have generated embryonic stem cells and transgenic mice with green fluorescent protein (GFP) inserted into the Pitx3 locus via homologous recombination. In the central nervous system, Pitx3 directed GFP was visualised ...

  7. Proton Pathways in Green Fluorescence Protein Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

    E-Print Network [OSTI]

    Agmon, Noam

    Proton Pathways in Green Fluorescence Protein Noam Agmon Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel ABSTRACT Proton pathways in green fluorescent protein pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton

  8. On the rough folding landscape of green fluorescent protein

    E-Print Network [OSTI]

    Andrews, Benjamin Thomas

    2008-01-01

    H. (2008). Understanding protein folding: small proteins inmultiple pathways of protein folding. Chem Biol 2, 255-60.Polymer principles and protein folding. Protein Sci 8, 1166-

  9. Fluorescent Protein Applications in Microscopy

    E-Print Network [OSTI]

    Straight, Aaron

    . The Identification of Green Fluorescent Protein III. Formation of the GFP Chromophore IV. The Structure of GFP V environment. II. The Identification of Green Fluorescent Protein The isolation of green fluorescent protein of Aequorea, Shimomura et al. noted that the lumines- cence from aequorin was blue rather than the green

  10. On the rough folding landscape of green fluorescent protein

    E-Print Network [OSTI]

    Andrews, Benjamin Thomas

    2008-01-01

    W. A. (2004). The protein folding 'speed limit'. CurrentG. (1997). Theory of protein folding: the energy landscapeenergy landscape of protein folding: a synthesis. Proteins

  11. Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and

    E-Print Network [OSTI]

    Jackson, David

    Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot Jackson¶ Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring between epidermal cells of Arabidopsis and onion. When expressed in vivo, the GFP KN1 fusion trafficked

  12. Chemistry & Biology Conversion of Red Fluorescent Protein

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    resonance energy transfer applications. INTRODUCTION Green fluorescent protein (GFP) from Aequoria victoria for multicolor and lifetime imaging, as well as the outstanding donor for green fluorescent proteins in Fo¨ rster green fluorescent probes, such as TagGFP or EGFP (Shaner et al., 2005). The improvement

  13. Engineering novel fluorescent proteins

    E-Print Network [OSTI]

    Shaner, Nathan Christopher

    2006-01-01

    to Choosing Fluorescent Proteins. Nat. Methods. 2 (12): 905-Dynamics of Z-band based proteins in developing skeletaland yellow fluorescent proteins derived from Discosoma sp.

  14. Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein

    SciTech Connect (OSTI)

    Arpino, James A. J. [Cardiff University, Park Place, Cardiff CF10 3AT Wales (United Kingdom); Rizkallah, Pierre J., E-mail: rizkallahp@cardiff.ac.uk [Cardiff University, Heath Park, Cardiff CF14 4XN Wales (United Kingdom); Jones, D. Dafydd, E-mail: rizkallahp@cardiff.ac.uk [Cardiff University, Park Place, Cardiff CF10 3AT Wales (United Kingdom)

    2014-08-01

    The beneficial engineered single-amino-acid deletion variants EGFP{sup D190?} and EGFP{sup A227?} have been studied. Single-amino-acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and prejudiced molecular understanding of mutations that shorten the protein backbone. Single-amino-acid deletion variants of enhanced green fluorescent protein (EGFP) have been identified by directed evolution with the beneficial effect of imparting increased cellular fluorescence. Biophysical characterization revealed that increased functional protein production and not changes to the fluorescence parameters was the mechanism that was likely to be responsible. The structure EGFP{sup D190?} containing a deletion within a loop revealed propagated changes only after the deleted residue. The structure of EGFP{sup A227?} revealed that a ‘flipping’ mechanism was used to adjust for residue deletion at the end of a ?-strand, with amino acids C-terminal to the deletion site repositioning to take the place of the deleted amino acid. In both variants new networks of short-range and long-range interactions are generated while maintaining the integrity of the hydrophobic core. Both deletion variants also displayed significant local and long-range changes in dynamics, as evident by changes in B factors compared with EGFP. Rather than being detrimental, deletion mutations can introduce beneficial structural effects through altering core protein properties, folding and dynamics, as well as function.

  15. Development of rapid microbial methods for lysine quantification in feed ingredients based on green fluorescent protein fluorescence 

    E-Print Network [OSTI]

    Chalova-Zhekova, Vesela I.

    2007-04-25

    ) was inserted in the genome of E. coli lysine auxotroph as a part of a mini-Tn5- transposon by conjugation. Bacterial growth response to external lysine and small peptides was monitored and recorded by measuring the fluorescence emitted by GFP. The second type...

  16. Characterization of the influence of 1-butyl-3-methylimidazolium chloride on the structure and thermal stability of green fluorescent protein

    SciTech Connect (OSTI)

    Heller, William T [ORNL; O'Neill, Hugh Michael [ORNL; Zhang, Qiu [ORNL; Baker, Gary A [ORNL

    2010-01-01

    Ionic liquids (ILs) are finding a vast array of applications as novel solvents for a wide variety of processes that include enzymatic chemistry, particularly as more biocompatible ILs are designed and discovered. While it is assumed that a native or near-native structure is required for enzymatic activity, there is some evidence that ILs alter protein structure and oligomerization states in a manner than can negatively impact function. The IL 1-butyl-3-methylimidazolium chloride, [bmim]Cl, is a well-studied, water-miscible member of the popular 1-alkyl-3-methylimidazolium IL family. To improve our understanding of the impact of water-miscible ILs on proteins, we have characterized the structure and oligomerization state of green fluorescent protein (GFP) in aqueous solutions containing 25 and 50 vol % [bmim]Cl using a combination of optical spectroscopy and small-angle neutron scattering (SANS). Measurements were also performed as a function of temperature to provide insight into the effect of the IL on the thermal stability of GFP. While GFP exists as a dimer in water, the presence of 25 vol % [bmim]Cl causes GFP to transition to a monomeric state. The SANS data indicate that GFP is a great deal less compact in 50 vol % [bmim]Cl than in neat water, indicative of unfolding from the native structure. The oligomerization state of the protein in IL-containing aqueous solution changes from a dimer to a monomer in response to the IL, but does not change as a function of temperature in the IL-containing solution. The SANS and spectroscopic results also demonstrate that the addition of [bmim]Cl to the solution decreases the thermal stability of GFP, allowing the protein to unfold at lower temperatures than in aqueous solution.

  17. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M. (Santa Fe, NM); Waldo, Geoffrey S. (Santa Fe, NM); Kiss, Csaba (Los Alamos, NM)

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  18. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M. (Santa Fe, NM); Waldo, Geoffrey S. (Santa Fe, NM); Kiss, Csaba (Los Alamos, NM)

    2011-11-29

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  19. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M. (Santa Fe, NM); Waldo, Geoffrey S. (Santa Fe, NM); Kiss, Csaba (Los Alamos, NM)

    2012-05-01

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  20. Chemistry & Biology Red Fluorescent Protein with Reversibly

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    to the photoswitchable absor- bance, rsTagRFP can be used as an acceptor for a photochromic Fo¨ rster resonance energy-OFF photoswitching of the rsTagRFP acceptor. INTRODUCTION Green fluorescent protein (GFP) from Aequorea victoriaTFP0.7 (Henderson et al., 2007), green Dronpa (Ando et al., 2004) and its derivatives (Ando et al

  1. Improved ``Optical Highlighter'' Probes Derived from Discosoma Red Fluorescent Protein

    E-Print Network [OSTI]

    Marchant, Jonathan

    , Minneapolis, Minnesota ABSTRACT The tetrameric red fluorescent protein, DsRed, undergoes a rapid red to green-emitting species of DsRed and an enhancement of emission from the ``immature'' green species, likely caused by dequenching of fluorescence resonance energy transfer occurring within the protein tetramer. Here, we have

  2. Modeling green fluorescent protein transcription, translation and modification as a method to obtain NF-kappaB activation profiles 

    E-Print Network [OSTI]

    Laible, Allyson Marie

    2009-05-15

    that fluorescence increases up to 24 hours after an initial delay of approximately four hours. The fluorescence data was also used to develop a model describing significant events leading to NF-?B activation and GFP expression. In addition, a model describing...

  3. Comparative Studies on the Structure and Stability of Fluorescent Proteins EGFP, zFP506, mRFP1, "dimer2", and DsRed1

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    -like fluorescent proteins, we have undertaken a systematic analysis of series of green and red fluorescent proteins with different association states. The list of studied proteins includes EGFP (green monomer), zFP506 (green). Cloning of green fluorescent protein (GFP)1 from the jellyfish Aequorea Victoria (class Hydrozoa) (2

  4. Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins

    E-Print Network [OSTI]

    Campbell, Robert E.

    Proteins Hui-wang Ai, Nathan C. Shaner,§,| Zihao Cheng, Roger Y. Tsien,§ and Robert E. Campbell*, Uni: The variant of Aequorea green fluorescent protein (GFP) known as blue fluorescent protein (BFP) was originally improved versions of BFP along with a variety of engineered fluorescent protein variants with novel

  5. Teal fluorescent proteins: Characterization of a reversibly photoswitchable variant

    E-Print Network [OSTI]

    Campbell, Robert E.

    . Cyan FPs (CFPs), widely used in multicolor imaging or as a fluorescence resonance energy transfer (FRET green fluorescent protein (GFP) in 1961 when he was isolating aequorin, a calcium cell imaging due to drawbacks such as dual excitation peaks and poor folding efficiency. Fortunately

  6. Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein John T. M. Kennis,*,

    E-Print Network [OSTI]

    van Stokkum, Ivo

    States *S Supporting Information ABSTRACT: Cyan, green, yellow, and red fluorescent proteins (FPs) homologous to green fluorescent protein (GFP) are used extensively as model systems to study fundamental processes in photobiology, such as the capture of light energy by protein-embedded chromophores, color

  7. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  8. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2011-06-07

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  9. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM)

    2007-09-18

    The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

  10. Protein- protein interaction detection system using fluorescent protein microdomains

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2010-02-23

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  11. Protein subcellular localization assays using split fluorescent proteins

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2009-09-08

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  12. Blue-green phosphor for fluorescent lighting applications

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  13. Fluorescent protein marker lines in maize: generation and applications

    E-Print Network [OSTI]

    Jackson, David

    : fluorescent protein, FRET, maize, pOp, LhG4 Introduction Maize, one of the most important crops worldwide, has

  14. LucY: A versatile new fluorescent reporter protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Auldridge, Michele E.; Cao, Hongnan; Sen, Saurabh; Franz, Laura P.; Bingman, Craig A.; Yennamalli, Ragothaman M.; Phillips, Jr., George N.; Mead, David; Steinmetz, Eric J.; Michnick, Stephen W.

    2015-04-23

    We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276nm, 377nm, and 460nm and a single emission peak at 530nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrastmore »to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.« less

  15. Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    CHAPTER 17 Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry Kiryl D. Piatkevich. Simultaneous Detection of Multiple FPs V. Fluorescent Timers VI. FRET-Based Genetically Encoded Biosensors A. Conventional FRET Pairs B. Novel Advanced FRET Pairs C. FRET Biosensors in Multicolor Flow Cytometry D

  16. In Vitro Transport of a Fluorescent Nuclear Protein and Exclusion of Non-Nuclear Proteins

    E-Print Network [OSTI]

    Forbes, Douglass

    In Vitro Transport of a Fluorescent Nuclear Protein and Exclusion of Non-Nuclear Proteins Donald D microscopic assay for nuclear transport. The assay uses an extract of Xenopus eggs, normal or synthetic nuclei, and a fluorescently labeled nuclear protein, nucleoplasmin. This in vitro system accurately mimics in vivo nuclear

  17. A FRET-Facilitated Photoswitching Using an Orange Fluorescent Protein with the Fast Photoconversion Kinetics

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    resonance energy transfer (FRET) from green fluorescent donors. We have termed the latter effect a FRET available photoconvertible green- to-red probes are in high demand. We have developed a monomericEos2,4 Kaede,5 mKikGR,6 mClavGR27 and their derivatives, change their fluorescent color from green

  18. Yellow fluorescent protein phiYFPv (Phialidium): structure and structure-based mutagenesis

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z., E-mail: vzpletnev@gmail.com; Souslova, Ekaterina; Chudakov, Dmitry M. [Russian Academy of Sciences, Moscow (Russian Federation); Lukyanov, Sergey [Russian Academy of Sciences, Moscow (Russian Federation); Nizhny Novgorod State Medical Academy, Nizhny Novgorod (Russian Federation); Martynov, Vladimir I.; Arhipova, Svetlena; Artemyev, Igor [Russian Academy of Sciences, Moscow (Russian Federation); Wlodawer, Alexander [National Cancer Institute, Frederick, MD 21702 (United States); Dauter, Zbigniew [National Cancer Institute, Argonne, IL 60439 (United States); Pletnev, Sergei [National Cancer Institute, Argonne, IL 60439 (United States); SAIC-Frederick, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Russian Academy of Sciences, Moscow (Russian Federation)

    2013-06-01

    The yellow fluorescent protein phiYFPv with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The yellow fluorescent protein phiYFPv (?{sub em}{sup max} ? 537 nm) with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The latter fluorescent protein is one of only two known cases of naturally occurring proteins that exhibit emission spectra in the yellow–orange range (535–555 nm). Here, the crystal structure of phiYFPv has been determined at 2.05 Å resolution. The ‘yellow’ chromophore formed from the sequence triad Thr65-Tyr66-Gly67 adopts the bicyclic structure typical of fluorophores emitting in the green spectral range. It was demonstrated that perfect antiparallel ?-stacking of chromophore Tyr66 and the proximal Tyr203, as well as Val205, facing the chromophore phenolic ring are chiefly responsible for the observed yellow emission of phiYFPv at 537 nm. Structure-based site-directed mutagenesis has been used to identify the key functional residues in the chromophore environment. The obtained results have been utilized to improve the properties of phiYFPv and its homologous monomeric biomarker tagYFP.

  19. Enhanced Archaerhodopsin Fluorescent Protein Voltage Yiyang Gong1,2*

    E-Print Network [OSTI]

    Ferrari, Silvia

    Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators Yiyang Gong1,2* , Jin Zhong Li1 by nearly three-fold in comparison to Arch-D95N. Citation: Gong Y, Li JZ, Schnitzer MJ (2013) Enhanced; Published June 19, 2013 Copyright: © 2013 Gong et al. This is an open-access article distributed under

  20. Rotational order–disorder structure of fluorescent protein FP480

    SciTech Connect (OSTI)

    Pletnev, Sergei, E-mail: svp@ncifcrf.gov [SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Morozova, Kateryna S.; Verkhusha, Vladislav V. [Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461 (United States); Dauter, Zbigniew, E-mail: svp@ncifcrf.gov [Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States)

    2009-09-01

    An analysis of the rotational order–disorder structure of fluorescent protein FP480 is presented. In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

  1. Extending the utility of enzymes for site-specific targeting of fluorescent probes

    E-Print Network [OSTI]

    Liu, Daniel S. (Daniel Shao-Chen)

    2014-01-01

    Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, ...

  2. Interdomain Fluorescence Resonance Energy Transfer in SERCA Probed by Cyan-Fluorescent Protein Fused to the Actuator Domain

    E-Print Network [OSTI]

    Thomas, David D.

    ), the measured FRET efficiency between CFP (donor in A domain) and FITC (acceptor in N domain) was 0.34 ( 0Interdomain Fluorescence Resonance Energy Transfer in SERCA Probed by Cyan-Fluorescent Protein transport by the sarcoendoplasmic reticulum Ca-ATPase (SERCA) from rabbit fast- twitch skeletal muscle. X

  3. Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Deng, Junjing; Vine, David J.; Chen, Si; Nashed, Youssef S. G.; Jin, Qiaoling; Phillips, Nicholas W.; Peterka, Tom; Ross, Rob; Vogt, Stefan; Jacobsen, Chris J.

    2015-02-24

    Trace metals play important roles in normal and in disease-causing biological functions. X-ray fluorescence microscopy reveals trace elements with no dependence on binding affinities (unlike with visible light fluorophores) and with improved sensitivity relative to electron probes. However, X-ray fluorescence is not very sensitive for showing the light elements that comprise the majority of cellular material. Here we show that X-ray ptychography can be combined with fluorescence to image both cellular structure and trace element distribution in frozen-hydrated cells at cryogenic temperatures, with high structural and chemical fidelity. Ptychographic reconstruction algorithms deliver phase and absorption contrast images at a resolutionmore »beyond that of the illuminating lens or beam size. Using 5.2-keV X-rays, we have obtained sub–30-nm resolution structural images and ~90-nm–resolution fluorescence images of several elements in frozen-hydrated green algae. This combined approach offers a way to study the role of trace elements in their structural context.« less

  4. Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein

  5. Cyanine-based probe\\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

    SciTech Connect (OSTI)

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2013-01-15

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  6. Fluorescent-Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle

    E-Print Network [OSTI]

    Campbell, Robert E.

    Fluorescent-Protein-Based Biosensors: Modulation of Energy Transfer as a Design Principle Robert E. Campbell University of Alberta Genetically-encoded biosensors based on FRET between fluorescent proteins.acs.org/journal/ancham.) The definition of a "biosensor"sthat is, a detection system that relies on a biomolecule for molecular

  7. X-ray crystal structure and properties of Phanta, a weakly fluorescent photochromic GFP-like protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Paul, Craig Don; Traore, Daouda A. K.; Olsen, Seth; Devenish, Rodney J.; Close, Devin W.; Bell, Toby D. M.; Bradbury, Andrew; Wilce, Matthew C. J.; Prescott, Mark

    2015-04-29

    Phanta is a reversibly photoswitching chromoprotein (?F, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (?F, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in combination, to the optical properties of Phanta. Single amino acid substitutions at position 193 resulted in proteins with very low ?F, indicating the importance of this position in controlling the fluorescence efficiency of the variant proteins. The substitution Thr69Val in Phanta was important for supressing the formation of a protonated chromophore species observed in some His193 substituted variants,more »whereas the substitution Gln62Met did not significantly contribute to the useful optical properties of Phanta. X-ray crystal structures for Phanta (2.3 Å), eCGP123T69V (2.0 Å) and eCGP123H193Q (2.2 Å) in their non-photoswitched state were determined, revealing the presence of a cis-coplanar chromophore. We conclude that changes in the hydrogen-bonding network supporting the cis-chromophore, and its contacts with the surrounding protein matrix, are responsible for the low fluorescence emission of eCGP123 variants containing a His193 substitution.« less

  8. Fluorescence Microscopy Evidence for Quasi-Permanent Attachment of Antifreeze Proteins to Ice Surfaces

    E-Print Network [OSTI]

    Wettlaufer, John S.

    Fluorescence Microscopy Evidence for Quasi-Permanent Attachment of Antifreeze Proteins to Ice ABSTRACT Many organisms are protected from freezing by the presence of extracellular antifreeze proteins (AFPs), which bind to ice, modify its morphology, and prevent its further growth. These proteins have

  9. Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies

    E-Print Network [OSTI]

    Croquette, Vincent

    Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies for protein folding studies and has been extensively stud- ied, both experimentally (at the ensemble level concentration. It is shown that new infor- mation about different aspects of the protein folding reaction can

  10. Genetically encoded biosensors based on engineered fluorescent proteins Wolf B. Frommer, Michael W. Davidson, Robert Campbell

    E-Print Network [OSTI]

    Campbell, Robert E.

    Genetically encoded biosensors based on engineered fluorescent proteins Wolf B. Frommer, Michael W. Davidson, Robert Campbell Supplementary Table I. List of biosensors based on single polypeptide chains Biosensor specificity Representative biosensor(s) Reference(s) Translocation-based biosensors

  11. Using co-cultures expressing fluorescence resonance energy transfer based protein biosensors to simultaneously image caspase-3 and Ca2+

    E-Print Network [OSTI]

    Using co-cultures expressing fluorescence resonance energy transfer based protein biosensors received: 8 June 2005; Accepted 11 June 2005 Key words: biosensor, fluorescence imaging, fluorescence)-based protein biosensors allow the spatial and temporal imaging of signaling events in living cells. However

  12. Single-Molecule Fluorescence Studies of Protein Folding and Conformational Xavier Michalet,* Shimon Weiss, and Marcus Jager*

    E-Print Network [OSTI]

    Michalet, Xavier

    Single-Molecule Fluorescence Studies of Protein Folding and Conformational Dynamics Xavier Michalet. Single-Molecule Fluorescence Studies of Protein Folding and Conformations at Equilibrium 1796 4-Molecule Protein Folding under Nonequilibrium Conditions 1808 6. Conclusion 1809 7. Acknowledgments 1810 8

  13. Mobility of Adsorbed Proteins Studied by Fluorescence Recovery after Photobleaching

    E-Print Network [OSTI]

    Velev, Orlin D.

    microscopy (SPM), of the two-dimen- sional structure of the adsorbed layer. The adsorbed protein molecules

  14. green

    National Nuclear Security Administration (NNSA)

    0%2A en Nevada National Security Site operator recognized for green fleet http:www.nnsa.energy.govblognevada-national-security-site-operator-recognized-green-fleet

    The...

  15. Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine

    SciTech Connect (OSTI)

    Pletnev, Vladimir Z., E-mail: vzpletnev@gmail.com; Pletneva, Nadya V.; Lukyanov, Konstantin A.; Souslova, Ekaterina A.; Fradkov, Arkady F.; Chudakov, Dmitry M.; Chepurnykh, Tatyana; Yampolsky, Ilia V. [Russian Academy of Sciences, Moscow (Russian Federation); Wlodawer, Alexander [National Cancer Institute, Frederick, MD 21702 (United States); Dauter, Zbigniew [National Cancer Institute, Argonne, IL 60439 (United States); Pletnev, Sergei, E-mail: vzpletnev@gmail.com [National Cancer Institute, Argonne, IL 60439 (United States); SAIC-Frederick, Argonne, IL 60439 (United States); Russian Academy of Sciences, Moscow (Russian Federation)

    2013-09-01

    The crystal structure of the novel red emitting fluorescent protein from lancelet Branchiostoma lanceolatum (Chordata) revealed an unusual five residues cyclic unit comprising Gly58-Tyr59-Gly60 chromophore, the following Phe61 and Tyr62 covalently bound to chromophore Tyr59. A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the ‘core’ structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (?{sub ex}/?{sub em} = 502/511 nm) and red laRFP (?{sub ex}/?{sub em} ? 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-?S83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ?20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C{sup ?} atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (?70 nm) of laRFP was verified by extensive structure-based site-directed mutagenesis.

  16. Cyanine-based probe\\tag-peptide pair for fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana (Richland, WA); Cao, Haishi (Richland, WA)

    2010-08-17

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  17. Use of green fluorescent protein for the analysis of protein-protein and protein-DNA interactions 

    E-Print Network [OSTI]

    Chen, Kai

    2011-01-01

    Restriction modification (RM) systems play a crucial role in preventing the entry of foreign DNA into the bacterial cell. The best studied Type I RM system is EcoKI from Escherichia coli K12. Both bacteriophage and ...

  18. Development and analysis of recombinant fluorescent probes for use in live cell imaging of filamentous fungi 

    E-Print Network [OSTI]

    Altenbach, Kirsten

    2010-01-01

    The molecular cloning and subsequent engineering of the green fluorescent protein (GFP) of the jellyfish Aequoria victoria allowed a novel approach to the investigation of cell signalling. GFP and its mutants can now not ...

  19. Effect of enhanced Renilla luciferase and fluorescent protein variants on the Foerster distance of Bioluminescence resonance energy transfer (BRET)

    SciTech Connect (OSTI)

    Dacres, Helen, E-mail: helen.dacres@csiro.au [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)] [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia); Michie, Michelle; Wang, Jian [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)] [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia); Pfleger, Kevin D.G. [Laboratory for Molecular Endocrinology-GPCRs, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, The University of Western Australia, Perth (Australia)] [Laboratory for Molecular Endocrinology-GPCRs, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, The University of Western Australia, Perth (Australia); Trowell, Stephen C. [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)] [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)

    2012-08-31

    Highlights: Black-Right-Pointing-Pointer First experimental determination of Foerster distance (R{sub 0}) for enhanced BRET systems. Black-Right-Pointing-Pointer Effect of brighter BRET components RLuc2, RLuc8 and Venus was assessed. Black-Right-Pointing-Pointer Using brighter BRET components substantially increased (25%) R{sub 0} of the BRET{sup 1} system. Black-Right-Pointing-Pointer Using brighter BRET components marginally increased (2-9%) R{sub 0} of the BRET{sup 2} system. Black-Right-Pointing-Pointer Brighter BRET components improve the different weaknesses of BRET{sup 1} and BRET{sup 2} systems. -- Abstract: Bioluminescence resonance energy transfer (BRET) is an important tool for monitoring macromolecular interactions and is useful as a transduction technique for biosensor development. Foerster distance (R{sub 0}), the intermolecular separation characterized by 50% of the maximum possible energy transfer, is a critical BRET parameter. R{sub 0} provides a means of linking measured changes in BRET ratio to a physical dimension scale and allows estimation of the range of distances that can be measured by any donor-acceptor pair. The sensitivity of BRET assays has recently been improved by introduction of new BRET components, RLuc2, RLuc8 and Venus with improved quantum yields, stability and brightness. We determined R{sub 0} for BRET{sup 1} systems incorporating novel RLuc variants RLuc2 or RLuc8, in combination with Venus, as 5.68 or 5.55 nm respectively. These values were approximately 25% higher than the R{sub 0} of the original BRET{sup 1} system. R{sub 0} for BRET{sup 2} systems combining green fluorescent proteins (GFP{sup 2}) with RLuc2 or RLuc8 variants was 7.67 or 8.15 nm, i.e. only 2-9% greater than the original BRET{sup 2} system despite being {approx}30-fold brighter.

  20. ERK's Got Rhythm: Protein Oscillates in Breast Cells

    SciTech Connect (OSTI)

    None

    2009-11-01

    Researchers fluorescently-labeled the protein ERK and found it cycles between the nucleus and the cell proper of these breast cells over about 7 hours, causing them to pulsate green.

  1. Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same

    DOE Patents [OSTI]

    Tsien, Roger Y. (La Jolla, CA); Wang, Lei (San Diego, CA)

    2008-07-01

    Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

  2. Structural Evidence for a Dehydrated Intermediate in Green Fluorescent Protein Chromophore Biosynthesis*

    E-Print Network [OSTI]

    Biosynthesis* Received for publication,December 6, 2009, and in revised form, February 23, 2010 Published, JBC- zolone and phenolic rings, has been attributed to one of the intermediate states in the GFP chromophore biosynthesis. The UV irradiation ( 250­300 nm) of aceGFP-G222E drives the chromophore maturation further

  3. Green fluorescent protein based indicators of dynamic redox changes and reactive oxygen species

    E-Print Network [OSTI]

    Dooley, Colette

    2006-01-01

    CA), The codon sequence VGB was used to in the forward andnucleotide codon sequence (VGB which encodes for G,C,A at147 was varied by using VGB codon sequence. Proline at

  4. Green fluorescent protein based indicators of dynamic redox changes and reactive oxygen species

    E-Print Network [OSTI]

    Dooley, Colette

    2006-01-01

    tetrachlorodibenzo-p-dioxin or indolo(3,2-b)carbazole isTetrachlorodibenzo-p-dioxin induced cytochrome P450s alterTetrachlorodibenzo-p-dioxin (TCDD, 4 and 100 nM), for 24

  5. The extremely slow-exchanging core and acid-denatured state of green fluorescent protein

    E-Print Network [OSTI]

    Jackson, Sophie

    with a complex eleven- stranded -barrel structure. Previous studies have shown that it has a complex energy resonance ,,NMR... techniques to probe the energy landscape for folding of GFP in further detail. H a folding nucleus. Using a pulsed-labeling strategy, the acid-denatured state has been investigated

  6. Near-infrared fluorescence glucose sensing based on glucose/galactose-binding protein coupled to 651-Blue Oxazine

    SciTech Connect (OSTI)

    Khan, Faaizah; Pickup, John C.

    2013-08-30

    Highlights: •We showed that the NIR fluorophore, 651-Blue Oxazine, is solvatochromic (polarity sensitive). •Blue Oxazine was covalently attached to mutants of glucose/galactose-binding protein (GBP). •Fluorescence intensity of GBP-Blue Oxazine increased with addition of glucose. •Fluorescence from bead-immobilised GBP-Blue Oxazine was detectable through skin in vitro. •This shows proof-of-concept for non-invasive glucose sensing using GBP-Blue Oxazine. -- Abstract: Near-infrared (NIR) fluorescent dyes that are environmentally sensitive or solvatochromic are useful tools for protein labelling in in vivo biosensor applications such as glucose monitoring in diabetes since their spectral properties are mostly independent of tissue autofluorescence and light scattering, and they offer potential for non-invasive analyte sensing. We showed that the fluorophore 651-Blue Oxazine is polarity-sensitive, with a marked reduction in NIR fluorescence on increasing solvent polarity. Mutants of glucose/galactose-binding protein (GBP) used as the glucose receptor were site-specifically and covalently labelled with Blue Oxazine using click chemistry. Mutants H152C/A213R and H152C/A213R/L238S showed fluorescence increases of 15% and 21% on addition of saturating glucose concentrations and binding constants of 6 and 25 mM respectively. Fluorescence responses to glucose were preserved when GBP-Blue Oxazine was immobilised to agarose beads, and the beads were excited by NIR light through a mouse skin preparation studied in vitro. We conclude GBP-Blue Oxazine shows proof-of-concept as a non-invasive continuous glucose sensing system.

  7. The rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP

    SciTech Connect (OSTI)

    Pletnev, Sergei, E-mail: pletnevs@mail.nih.gov [Argonne National Laboratory, Argonne, IL 60439 (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); Subach, Fedor V.; Verkhusha, Vladislav V. [Albert Einstein College of Medicine, Bronx, NY 10461 (United States); Dauter, Zbigniew, E-mail: pletnevs@mail.nih.gov [Argonne National Laboratory, Argonne, IL 60439 (United States)

    2014-01-01

    An analysis of the rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP is presented. The rotational order–disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order–disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.

  8. Quantitative Modeling and Estimation in Systems Biology using Fluorescent Reporter Systems 

    E-Print Network [OSTI]

    Bansal, Loveleena

    2013-12-10

    FIM Fischer Information Matrix FP Fluorescent Protein GFP Green Fluorescent Protein GLS Generalized Least Squares IBVP Intital Boundary Value Problem IL-6 Interleukin-6 IPDE Integro Partial Differential Equation LHS Latin Hypercube... Sampling MSE Mean Squared Error nM Nano Molar O.D. Optical Density ODE Ordinary Differential Equation PBE Population Balance Equation PBM Population Balance Model RE Relative Error S.D. Standard Deviation STAT3 Signal transducer...

  9. Development of Fluorescent Reporters for Monitoring Fe-S Cluster Transfer Reactions and Discerning the Role of Glutaredoxin Proteins in Fe-S Cluster Assembly 

    E-Print Network [OSTI]

    Vranish, James Nicholas

    2015-04-27

    and persulfides to form clusters). The in vitro study of cluster transfer has been hampered by the lack of sufficient methods to investigate the kinetics of these processes. We have developed fluorescently labeled iron-sulfur cluster binding proteins...

  10. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Dougalas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2003-12-09

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  11. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Douglas C. (San Jose, CA); Silva, Christopher M. (Sunnyvale, CA); Bylina, Edward J. (San Jose, CA); Coleman, William J. (Moutain View, CA); Dilworth, Michael R. (Santa Cruz, CA); Yang, Mary M. (San Jose, CA)

    2002-09-24

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  12. Investigation of molecular mechanisms of action of chelating drugs on protein-lipid model membranes by X-ray fluorescence

    SciTech Connect (OSTI)

    Novikova, N. N., E-mail: nn_novikova@ns.crys.ras.ru [Kurchatov Institute, Russian Research Center (Russian Federation); Zheludeva, S. I.; Koval'chuk, M. V.; Stepina, N. D. [Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation); Erko, A. I. [Berlin Electron Storage Ring Company for Synchrotron Radiation (Germany); Yur'eva, E. A. [Ministry of Health of the Russian Federation, Moscow Research Institute of Pediatrics and Pediatric Surgery (Russian Federation)

    2009-12-15

    Protein-lipid films based on the enzyme alkaline phosphatase were subjected to the action of chelating drugs, which are used for accelerating the removal of heavy metals from the human body, and the elemental composition of the resulting films was investigated. Total-reflection X-ray fluorescence measurements were performed at the Berlin Electron Storage Ring Company for Synchrotron Radiation (BESSY) in Germany. A comparative estimation of the protective effect of four drugs (EDTA, succimer, xydiphone, and mediphon) on membrane-bound enzymes damaged by lead ions was made. The changes in the elemental composition of the protein-lipid films caused by high doses of chelating drugs were investigated. It was shown that state-of-the-art X-ray techniques can, in principle, be used to develop new methods for the in vitro evaluation of the efficiency of drugs, providing differential data on their actions.

  13. BD Living ColorsTM DsRed-Monomer Fluorescent Protein

    E-Print Network [OSTI]

    Lebendiker, Mario

    protein · Ideal for fusion tag applications · Validated with a wide variety of fusion proteins · Perfect rapidly and is readily detected in 12 hours. The use of DsRed-Monomer as a fusion tag has been validated has been confirmed by several independent methods. When recombinant DsRed-Monomer is analyzed by FPLC

  14. Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z.; Shemiakina, Irina I.; Chudakov, Dmitriy M.; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei (Russ. Acad. Sci.); (SAIC); (NCI)

    2012-08-10

    The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, {lambda}{sub ex} = 552 nm, {lambda}{sub em} = 578 nm) and its bright far-red fluorescent variant Katushka ({lambda}{sub ex} = 588 nm, {lambda}{sub em} = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 {angstrom} at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f.

  15. Calculation of Transition Dipole Moment in Fluorescent ProteinsTowards Efficient Energy Transfer

    E-Print Network [OSTI]

    Baer, Roi

    representative of each fluorophore (Table 4 in the text, 4th column in bold). Coordinates in that case are taken therefore, to examine the impact of the geometry and in particular the symmetry on the resulting TDM. Table from the crystallographic data as published in the Protein Data Bank (see Table S2) with no further

  16. Development of a Green Fluorescent Protein-Based Cell Bioassay for the Rapid and Inexpensive Detection and Characterization

    E-Print Network [OSTI]

    Hammock, Bruce D.

    inducing potency of a series of previously uncharac- terized dioxin surrogates. This bioassay system has of use, low reagent cost, and application for high-throughput screening. Key Words: dioxin, Ah receptor (dioxin-responsive elements or DREs) (Deni- son et al., 1998a; Whitlock, 1999). Although ligands for Ah

  17. An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors

    E-Print Network [OSTI]

    Ghosh, Indraneel

    genetically encoded sensors7 based upon tethered green fluorescent protein (GFP) variants have been designedAn Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors Sujan S. Shekhawat for the design of a family of genetically encoded turn-on protease biosensors. In our design, an autoinhibited

  18. Ultraviolet resonance Raman and fluorescence studies of folded and unfolded conformations of the membrane protein OmpA

    E-Print Network [OSTI]

    Sanchez, Katheryn Marie

    2010-01-01

    Thermodynamics of Membrane Protein Folding: Lessons from theA Model for Membrane Protein Folding”, H. S. Shafaat, K. M.goals of membrane protein folding studies is to ascertain

  19. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of electronic equipment and computing resources. Energy Conservation Efficient Water Use & Management High Performance Sustainable Buildings Greening...

  20. Insight into the Common Mechanism of the Chromophore Formation in the Red Fluorescent Proteins: The Elusive Blue

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    : The Elusive Blue Intermediate Revealed Ksenia B. Bravaya, Oksana M. Subach, Nadezhda Korovina, Vladislav V calculations identifying the nature of a blue intermediate, a key species in the process of the red chromophore formation in DsRed, TagRFP, fluorescent timers, and PAmCherry. The chromophore of the blue intermediate has

  1. Millisecond timescale slimfield imaging and automated quantification of single fluorescent protein molecules for use in probing complex

    E-Print Network [OSTI]

    Leake, Mark C.

    protein (GFP),1 discovered in 1962 and now known to be utilized by many different ocean species which inhabit water depths where light struggles to penetrate. This natural form of GFP is excited maximally

  2. Distinct roles of the photosystem II protein PsbS and zeaxanthin in the regulation of light harvesting in plants revealed by fluorescence lifetime snapshots

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sylak-Glassman, Emily J.; Malnoë, Alizée; De Re, Eleonora; Brooks, Matthew D.; Fischer, Alexandra Lee; Niyogi, Krishna K.; Fleming, Graham R.

    2014-10-01

    The photosystem II (PSII) protein PsbS and the enzyme violaxanthin deepoxidase (VDE) are known to influence the dynamics of energy-dependent quenching (qE), the component of nonphotochemical quenching (NPQ) that allows plants to respond to fast fluctuations in light intensity. Although the absence of PsbS and VDE has been shown to change the amount of quenching, there have not been any measurements that can detect whether the presence of these proteins alters the type of quenching that occurs. The chlorophyll fluorescence lifetime probes the excited-state chlorophyll relaxation dynamics and can be used to determine the amount of quenching as well asmore »whether two different genotypes with the same amount of NPQ have similar dynamics of excited-state chlorophyll relaxation. We measured the fluorescence lifetimes on whole leaves of Arabidopsis thaliana throughout the induction and relaxation of NPQ for wild type and the qE mutants, npq4, which lacks PsbS; npq1, which lacks VDE and cannot convert violaxanthin to zeaxanthin; and npq1 npq4, which lacks both VDE and PsbS. These measurements show that although PsbS changes the amount of quenching and the rate at which quenching turns on, it does not affect the relaxation dynamics of excited chlorophyll during quenching. In addition, the data suggest that PsbS responds not only to ?pH but also to the ?? across the thylakoid membrane. In contrast, the presence of VDE, which is necessary for the accumulation of zeaxanthin, affects the excited-state chlorophyll relaxation dynamics.« less

  3. Green Chemistry and Workers

    E-Print Network [OSTI]

    2009-01-01

    of green chemistry and green engineering and by consideringResearch, Green Chemistry and Green Engineering Center, YaleOF GREEN CHEMISTRY AND GREEN ENGINEERING Julie Zimmerman By

  4. 428 BIOCHIMICAET BIOPHYSICAACTA pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE

    E-Print Network [OSTI]

    Govindjee

    428 BIOCHIMICAET BIOPHYSICAACTA BBA 46126 pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE on the "slow" (min) time course of Chlorophyll a fluorescence yield in the green alga Chlorella pyrenoidosa and in the blue-green alga Anacystis nidulans. In Chlorella, the decay of fluorescence yield, in the I- to 5-rain

  5. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Proteins Protein Engineering, Structure, and Function Los Alamos scientists seek a comprehensive understanding of the structure and function of proteins which can lead to a...

  6. Interaction between a plasma membrane-localized ankyrin-repeat protein ITN1 and a nuclear protein RTV1

    SciTech Connect (OSTI)

    Sakamoto, Hikaru; Sakata, Keiko; Kusumi, Kensuke; Kojima, Mikiko; Sakakibara, Hitoshi; Iba, Koh

    2012-06-29

    Highlights: Black-Right-Pointing-Pointer ITN1, a plasma membrane ankyrin protein, interacts with a nuclear DNA-binding protein RTV1. Black-Right-Pointing-Pointer The nuclear transport of RTV1 is partially inhibited by interaction with ITN1. Black-Right-Pointing-Pointer RTV1 can promote the nuclear localization of ITN1. Black-Right-Pointing-Pointer Both overexpression of RTV1 and the lack of ITN1 increase salicylic acids sensitivity in plants. -- Abstract: The increased tolerance to NaCl 1 (ITN1) protein is a plasma membrane (PM)-localized protein involved in responses to NaCl stress in Arabidopsis. The predicted structure of ITN1 is composed of multiple transmembrane regions and an ankyrin-repeat domain that is known to mediate protein-protein interactions. To elucidate the molecular functions of ITN1, we searched for interacting partners using a yeast two-hybrid assay, and a nuclear-localized DNA-binding protein, RTV1, was identified as a candidate. Bimolecular fluorescence complementation analysis revealed that RTV1 interacted with ITN1 at the PM and nuclei in vivo. RTV1 tagged with red fluorescent protein localized to nuclei and ITN1 tagged with green fluorescent protein localized to PM; however, both proteins localized to both nuclei and the PM when co-expressed. These findings suggest that RTV1 and ITN1 regulate the subcellular localization of each other.

  7. Fig S1. Images of Two dimensional gel electrophoresis gels. The left image is for the untreated control; the right image is for Gm treated samples. Green represents protein

    E-Print Network [OSTI]

    Matin, A.C.

    is for the untreated control; the right image is for Gm treated samples. Green represents protein spot intensity from. #12; Fig S2. N-acetyl cysteine (NAC) dampens the lethal effect of Gm in the stationaryA strain are reproduced from Figure 4 for ease of comparison. *** (p Gm

  8. Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology

    E-Print Network [OSTI]

    Krueger, Andrew T.

    Fluorescence spectroscopy is a powerful tool for probing complex biological processes. The ubiquity of peptide–protein and protein–protein interactions in these processes has made them important targets for fluorescence ...

  9. NREL Discovers Novel Protein Interaction in Green Algae that Suggests New Strategies to Improve Hydrogen Photoproduction (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2011-02-01

    A research team at the National Renewable Energy Laboratory (NREL) discovered a specific interaction between the protein ferredoxin - responsible for distributing reductants from photosynthesis to different metabolic pathways - and the HYDA2 hydrogenase, suggesting a role for HYDA2 in photohydrogen production.

  10. Buying green

    E-Print Network [OSTI]

    Bradshaw, William B., II

    2006-01-01

    Green development has received much attention over the past decade, with the greatest interest coming from designers. However, the development and investment communities have been slower to adopt green principles, and the ...

  11. Green Houston 

    E-Print Network [OSTI]

    Spanjian, L.

    2012-01-01

    Laura Spanjian, Sustainability Director www.greenhoustontx.gov www.codegreenhouston.org www.houstongoc.org www.greenhoustontx.gov/houstondriveselectric Follow Houston, the green City greenhoustontx More information on all Green...

  12. Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    materials Environmental Sustainability Sustainability Goals Recycling Green Purchasing Pollution Prevention Reusing Water Feature Stories More Like This Environmental Management...

  13. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Proteins Scientists manipulate and mimic proteins for use in creating solutions for medicine, sustainable energy, and more Read caption + Los Alamos National Laboratory graduate...

  14. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, Richard I. (Santa Fe, NM); Edwards, Bradley C. (Los Alamos, NM); Buchwald, Melvin I. (Santa Fe, NM); Gosnell, Timothy R. (Santa Fe, NM)

    1995-01-01

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement.

  15. TCD Green Campus Green Campus

    E-Print Network [OSTI]

    O'Mahony, Donal E.

    School of Nursing & Midwifery (D'Olier Street) College Green/Foster Place South Dunlop Oriel House#12;DIAGRAM TCD Green Campus Policies Green Campus Committee 1. Energy, Climate Change & Greenhouse Gas Emissions 2. Waste Management, Recycling & Litter Reduction 3. Water and Wastewater 4. Sustainable

  16. Excitation Decay Pathways of Lhca Proteins: A Time-Resolved Fluorescence Study Janne A. Ihalainen,*, Roberta Croce, Tomas Morosinotto,, Ivo H. M. van Stokkum,

    E-Print Network [OSTI]

    van Stokkum, Ivo

    for photosystem I (PSI) in green plants, consists mainly of four polypeptides, Lhca1-4. We report room temperature-harvesting complex II (LHCII), the main peripheral antenna complex of photosystem II in green plants. The decay be due to "slow" energy transfer, but is instead assigned to an unrelaxed state of the pigment

  17. SHORT COMMUNICATION The Chlorophyll Fluorescence Ratio F735/F700

    E-Print Network [OSTI]

    Gitelson, Anatoly

    sylvatica L.), elm (Ulmus minor 1988; Dahn et al., 1992). In green leaves, about 90% of Miller), and wild of reabsorption of the Chl fluorescence was quan- titatively estimated (Dahn et al., 1992; Gunther et al

  18. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, R.I.; Edwards, B.C.; Buchwald, M.I.; Gosnell, T.R.

    1995-09-05

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement. 6 figs.

  19. Three-dimensional fluorescence lifetime tomography

    SciTech Connect (OSTI)

    Godavarty, Anuradha; Sevick-Muraca, Eva M.; Eppstein, Margaret J. [327 Votey Building, Department of Computer Science, University of Vermont, Burlington, Vermont 05405 (United States); 1011 Richardson Building, Photon Migration Laboratories, Texas A and M University, College Station, Texas 77843 (United States); 327 Votey Building, Department of Computer Science, University of Vermont, Burlington, Vermont 05405 (United States)

    2005-04-01

    Near-infrared fluorescence tomography using molecularly targeted lifetime-sensitive, fluorescent contrast agents have applications for early-stage cancer diagnostics. Yet, although the measurement of fluorescent lifetime imaging microscopy (FLIM) is extensively used in microscopy and spectroscopy applications, demonstration of fluorescence lifetime tomography for medical imaging is limited to two-dimensional studies. Herein, the feasibility of three-dimensional fluorescence-lifetime tomography on clinically relevant phantom volumes is established, using (i) a gain-modulated intensified charge coupled device (CCD) and modulated laser diode imaging system, (ii) two fluorescent contrast agents, e.g., Indocyanine green and 3-3'-Diethylthiatricarbocyanine iodide differing in their fluorescence lifetime by 0.62 ns, and (iii) a two stage approximate extended Kalman filter reconstruction algorithm. Fluorescence measurements of phase and amplitude were acquired on the phantom surface under different target to background fluorescence absorption (70:1, 100:1) and fluorescence lifetime (1:1, 2.1:1) contrasts at target depths of 1.4-2 cm. The Bayesian tomography algorithm was employed to obtain three-dimensional images of lifetime and absorption owing to the fluorophores.

  20. Greening Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    travel and commuting to and from work by 13 percent. Energy Conservation Efficient Water Use & Management High Performance Sustainable Buildings Greening...

  1. Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri

    E-Print Network [OSTI]

    Prochnik, Simon E.

    2011-01-01

    in the multicellular green alga Volvox carteri One-sentencegenome reveals that this green alga’s increased organismal16 P. Volvocine algae-specific protein

  2. Green Infrastructure 

    E-Print Network [OSTI]

    Tildwell, J.

    2011-01-01

    SWM, Green Buildings, Energy Forum, Texas Smartscape) ? Deteriorating Roadways ? ASCE Report Card on Texas Infrastructure for 2008 identified roads as the #1 infrastructure concern ? Congestion ? DFW congestion is growing over 45% faster than...? ? ASCE Sustainability ? Greenroads ? Green Streets ? SmartCode ? New York High Performance Infrastructure Guidelines ? Institute of Transportation Engineers ? American Public Works Association ? ?and many more. Planning ? City and Regional...

  3. Angiographic Method Using Green Porphyrinew In Primmate Eyes

    DOE Patents [OSTI]

    Miller, Joan W. (Boston, MA); Young, Lucy H.Y. (Boston, MA); Gragoudas, Evangelos S. (Boston, MA)

    1998-01-13

    An angiographic method to observe the condition of blood vessels, including neovasculature in the eyes of living primates using green porphyrins and light at a wavelength of 550-700 nm to effect fluorescence is disclosed.

  4. Better Enzymes for Biofuels and Green Chemistry: Solving the

    E-Print Network [OSTI]

    RESEARCH HIGHLIGHTS Better Enzymes for Biofuels and Green Chemistry: Solving the Cofactor Imbalance Better Enzymes for Biofuels and Green Chemistry: Solving the Cofactor Imbalance Problem Global-rational protein engineering approaches to drive industrial biocatalysis forward. Better Enzymes for Biofuels

  5. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass mapSpeedingProgramExemptionsProtein Dynamics Hit the BigProteinProteins Scientists

  6. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation of Fe(II) by Carbon-Rich MatricesstudentsProjectsPropertymaterialsProteins Protein

  7. Folding Reporter Not 4 Profit BMTA LANL Agreement Number: LOS ALAMOS NATIONAL SECURITY, LLC

    E-Print Network [OSTI]

    , Berendzen J, Terwilliger TC (1999) "Rapid protein folding assay using Green Fluorescent Protein" Nat Biotech

  8. Folding Reporter Foreign Not 4 Profit BMTA LANL Agreement Number: LOS ALAMOS NATIONAL SECURITY, LLC

    E-Print Network [OSTI]

    , Berendzen J, Terwilliger TC (1999) "Rapid protein folding assay using Green Fluorescent Protein" Nat Biotech

  9. Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements

    E-Print Network [OSTI]

    Liyanage, Mangala Roshan

    2009-01-01

    Calmodulin (CaM) is a calcium signaling protein that activates over hundred of targets including PMCA. This dissertation mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions...

  10. Green Weight 

    E-Print Network [OSTI]

    Al-Haji, A.; Al-Omair, A.

    2010-01-01

    Green-weight is a novel system designed to supply a high-rise building with all its needs of electricity using wind energy and to supplement its lighting needs with natural sunlight. David Fisher has proposed an ingenious method to harness the wind...

  11. Green Chemistry and Workers

    E-Print Network [OSTI]

    2009-01-01

    chemistry as it produces green, sustainable union jobs; •jobs and industries, green remediation, and green chemistry.jobs—including those connected to the emerging field of green chemistry—and

  12. GREEN ENGINEERING PROGRAM Green Engineering & Life

    E-Print Network [OSTI]

    Pennycook, Steve

    GREEN ENGINEERING PROGRAM Green Engineering & Life Cycle Assessment (LCA) at Virginia Tech Oak Ridge National Lab April 10, 2014 Dr. Sean McGinnis Director ­ VT Green Engineering Program Research Associate Professor ­ Materials Science & Engineering #12;GREEN ENGINEERING PROGRAM Virginia Tech College

  13. Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid you not findGeoscience/EnvironmentGlobalADDITIONAL RESOURCESGrantsGreen

  14. Greening Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation Current HABFESOpportunitiesNERSC GettingGraphene's 3DGreenGreenTransportation

  15. A component of green tea (-)-epigallocatechin-3-gallate, promotes apoptosis in T24 human bladder cancer cells via modulation of the PI3K/Akt pathway and Bcl-2 family proteins

    SciTech Connect (OSTI)

    Qin Jie [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Xie Liping [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China)]. E-mail: xielp@zjuem.zju.edu.cn; Zheng Xiangyi [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Wang Yunbin [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Bai Yu [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Shen Huafeng [Department of Urology, First Affiliated Hospital, Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province (China); Li Longcheng [Department of Urology, University of California San Francisco and Veteran Affairs Medical Center San Francisco, San Francisco, CA (United States); Dahiya, Rajvir [Department of Urology, University of California San Francisco and Veteran Affairs Medical Center San Francisco, San Francisco, CA (United States)

    2007-03-23

    Bladder cancer is the fourth most common cancer in men and ninth most common in women. It has a protracted course of progression and is thus an ideal candidate for chemoprevention strategies and trials. This study was conducted to evaluate the chemopreventive/antiproliferative potential of (-)-epigallocatechin gallate (EGCG, the major phytochemical in green tea) against bladder cancer and its mechanism of action. Using the T24 human bladder cancer cell line, we found that EGCG treatment caused dose- and time-dependent inhibition of cellular proliferation and cell viability, and induced apoptosis. Mechanistically, EGCG inhibits phosphatidylinositol 3'-kinase/Akt activation that, in turn, results in modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells. These findings suggest that EGCG may be an important chemoprevention agent for the management of bladder cancer.

  16. Green Manufacturing

    SciTech Connect (OSTI)

    Patten, John

    2013-12-31

    Green Manufacturing Initiative (GMI): The initiative provides a conduit between the university and industry to facilitate cooperative research programs of mutual interest to support green (sustainable) goals and efforts. In addition to the operational savings that greener practices can bring, emerging market demands and governmental regulations are making the move to sustainable manufacturing a necessity for success. The funding supports collaborative activities among universities such as the University of Michigan, Michigan State University and Purdue University and among 40 companies to enhance economic and workforce development and provide the potential of technology transfer. WMU participants in the GMI activities included 20 faculty, over 25 students and many staff from across the College of Engineering and Applied Sciences; the College of Arts and Sciences' departments of Chemistry, Physics, Biology and Geology; the College of Business; the Environmental Research Institute; and the Environmental Studies Program. Many outside organizations also contribute to the GMI's success, including Southwest Michigan First; The Right Place of Grand Rapids, MI; Michigan Department of Environmental Quality; the Michigan Department of Energy, Labor and Economic Growth; and the Michigan Manufacturers Technical Center.

  17. Fluorescent optical position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  18. Safe biodegradable fluorescent particles

    DOE Patents [OSTI]

    Martin, Sue I. (Berkeley, CA); Fergenson, David P. (Alamo, CA); Srivastava, Abneesh (Santa Clara, CA); Bogan, Michael J. (Dublin, CA); Riot, Vincent J. (Oakland, CA); Frank, Matthias (Oakland, CA)

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  19. Green Chemistry and Workers

    E-Print Network [OSTI]

    2009-01-01

    J. Warner. 1998. Green Chemistry: Theory and Practice. NewNew Science, Green Chemistry and Environmental Health.abstract.html 5. American Chemistry Council. 2003. Guide to

  20. Green exciplex emission from a bilayer light-emitting diode containing a rare earth ternary complex

    E-Print Network [OSTI]

    Huang, Yanyi

    Green exciplex emission from a bilayer light-emitting diode containing a rare earth ternary complex form 18 October 2001 Abstract A bilayer organic light-emitting diode using a blue-fluorescent yttrium

  1. Probing the denatured state ensemble with fluorescence 

    E-Print Network [OSTI]

    Alston, Roy Willis

    2004-09-30

    of these five proteins were studied and compared to the fluorescent properties of eight model compounds: N-acetyl-tryptophan-amide (NATA), N-acetyl-Ala-Trp-Ala-amide (AWA), N-acetyl-Ala-Ala-Trp-Ala-Ala-amide (AAWAA), and five pentapeptides based on the sequence...

  2. Fluorescent Labeling Reagents Optimized for Capillary Electrophoretic Separations 

    E-Print Network [OSTI]

    Estrada, Roy Tonacao, III

    2012-02-14

    molecules fluoresce, background noise from the separation matrix and other extraneous compounds is usually a problem. Moreover, UV lasers tend to be more expensive than those used in the visible region [8]. To go around the background noise problem..., excitation can be done in the visible or higher wavelengths. However, only a very small number of proteins have native fluorescence above the UV region. To effectively use LIF as a detection method, proteins have to be labeled with fluorophores that can...

  3. EPA Green Power Leadership Awards

    Broader source: Energy.gov [DOE]

    The U.S. Environmental Protection Agency (EPA) Green Power Leadership Awards recognize exceptional achievement among EPA Green Power Partners and among green power suppliers.

  4. Fluorescent Tube Lamps

    Broader source: Energy.gov [DOE]

    FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

  5. Green Energy Tax Credit

    Office of Energy Efficiency and Renewable Energy (EERE)

    In addition to the Green Energy Tax Credit, the Carbon Tax Credit is available. This is the only carbon tax credit in the United States and it provides "certified green energy supply chain...

  6. GreenSun Hawaii

    Broader source: Energy.gov [DOE]

    GreenSun Hawaii is a loan loss reserve fund developed using funds from the The American Recovery and Reinvestment Act of 2009 (ARRA). GreenSun Hawaii partners with banks and credit unions, making...

  7. Green Event Request Name: _______________________________________________________________________

    E-Print Network [OSTI]

    Lawrence, Rick L.

    Green Event Request Name fill out the following completely and have it emailed to our recycling coordinator, Logun Norris

  8. Green Jobs Training Center

    Broader source: Energy.gov [DOE]

    Provides an overview of the training available through the Green Jobs Training Center including certification courses and the apprenticeship program.

  9. Blue-green and green phosphors for lighting applications

    DOE Patents [OSTI]

    Setlur, Anant Achyut; Chandran, Ramachandran Gopi; Henderson, Claire Susan; Nammalwar, Pransanth Kumar; Radkov, Emil

    2012-12-11

    Embodiments of the present techniques provide a related family of phosphors that may be used in lighting systems to generate blue or blue-green light. The phosphors include systems having a general formula of: ((Sr.sub.1-zM.sub.z).sub.1-(x+w)A.sub.wCe.sub.x).sub.3(Al.sub.1-ySi.s- ub.y)O.sub.4+y+3(x-w)F.sub.1-y-3(x-w) (I), wherein 0fluorescent tubes, among others, to produce blue and blue/green light. Further, the phosphors may be used in blends with other phosphors, or in combined lighting systems, to produce white light suitable for illumination.

  10. Green Fund Report, Submitted to the Green University Planning Committee,

    E-Print Network [OSTI]

    Northern British Columbia, University of

    are complete and 6 are in-progress. The following tables show the grants that have been awarded. #12;3 Green1 Green Fund Report, 2010-2012 Submitted to the Green University Planning Committee, September 2012 #12;2 ABOUT THE GREEN FUND What is the Green Fund? The Green Fund is a reserve of money that is used

  11. Concentration in Green Design

    E-Print Network [OSTI]

    Shewchuk, Jonathan

    , energy, infrastructure or transport. Participants in this specialization area work closely with the GreenConcentration in Green Design Research and Education Opportunities Carnegie Mellon University Civil and Environmental Engineering www.ce.cmu.edu M.S. Concentration Green Design - Course Only Track As an extension

  12. The Princeton Review's GREEN COLLEGES

    E-Print Network [OSTI]

    Hammack, Richard

    .S. Green Building Council · 322 school profiles cover green highlights on the nation's most eco-friendly

  13. Active-space completely-renormalized equation-of-motion coupled-cluster formalism: Excited-state studies of green fluorescent protein, free-base porphyrin, and oligoporphyrin dimer

    SciTech Connect (OSTI)

    Kowalski, Karol; Krishnamoorthy, Sriram; Villa, Oreste; Hammond, Jeffrey R.; Govind, Niranjan

    2010-04-21

    The development of efficient parallel implementations of electronic structure methods enables not only the study of excited states of large molecular systems but also a unique opportunity to assess the role of various correlation effects in describing excitation energies for systems composed of hundreds of electrons. In this article, we discuss the impact of triply excited configurations in Equation-of-Motion Coupled Cluster (EOMCC) formalism. As a benchmark system we chose the fused porphyrin dimer, which is described by the basis set composed of 942 functions and where 270 electrons were correlated in the EOMCC calculations.

  14. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen R. (Princeton, NJ); Sun, Yiru (Princeton, NJ); Giebink, Noel (Princeton, NJ); Thompson, Mark E. (Anaheim Hills, CA)

    2009-01-06

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  15. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen (Ann Arbor, MI); Sun, Yiru (Princeton, NJ); Giebink, Noel (Ann Arbor, MI); Thompson, Mark E. (Anaheim Hills, CA)

    2010-08-03

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  16. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are aminomore »acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.« less

  17. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    SciTech Connect (OSTI)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

  18. Connecting Stakeholders, Achieving Green 

    E-Print Network [OSTI]

    Rouse, S.; Nolan, B.

    2008-01-01

    Energy @ Work File: Green Paper Berlin Edition - R7 9092008 ? 2008 Page: 2/14 Contact: Scott.Rouse@Energy-Efficiency.com, 416 402-0525 This paper focuses on the following necessary conditions for commercial properties to reach their Green potential...% ?economic? potential from energy efficiency measures (EEM) with comparatively little risk. 1.2.2 Environmental Green House Gas (GHG) emission reduction solutions have become a public priority. Canada?s commercial facilities? direct and indirect...

  19. Green Power Offer (Maine)

    Broader source: Energy.gov [DOE]

    This chapter establishes requirements, standards and procedures and a competitive bidding process to implement the green power offer program. The program is designed to make renewable energy...

  20. Lumens Placard (Green) | Department of Energy

    Energy Savers [EERE]

    Green) Lumens Placard (Green) File lumensplacard-green.eps More Documents & Publications Lumens Placard (Green) Lumens Placard (Black) Lumens Placard (Black...

  1. Fluorescent image tracking velocimeter

    DOE Patents [OSTI]

    Shaffer, Franklin D. (Library, PA)

    1994-01-01

    A multiple-exposure fluorescent image tracking velocimeter (FITV) detects and measures the motion (trajectory, direction and velocity) of small particles close to light scattering surfaces. The small particles may follow the motion of a carrier medium such as a liquid, gas or multi-phase mixture, allowing the motion of the carrier medium to be observed, measured and recorded. The main components of the FITV include: (1) fluorescent particles; (2) a pulsed fluorescent excitation laser source; (3) an imaging camera; and (4) an image analyzer. FITV uses fluorescing particles excited by visible laser light to enhance particle image detectability near light scattering surfaces. The excitation laser light is filtered out before reaching the imaging camera allowing the fluoresced wavelengths emitted by the particles to be detected and recorded by the camera. FITV employs multiple exposures of a single camera image by pulsing the excitation laser light for producing a series of images of each particle along its trajectory. The time-lapsed image may be used to determine trajectory and velocity and the exposures may be coded to derive directional information.

  2. About Singapore Green Building Council

    E-Print Network [OSTI]

    - friendly, energy efficiency building materials, design and architecture ­ reaching out to Green buildingAbout Singapore Green Building Council About SGBC Green Building Conference Conference Programme Green Building Conference In line with the mission of the Singapore Green Building Council (SGBC

  3. The Green Gazette February 2013

    E-Print Network [OSTI]

    Harman, Neal.A.

    The Green Gazette February 2013 13 (number of Green Impact teams 2011/12) 27+ (number of Green Impact teams 2012/13) More than twice as many greening actions being carried out across campus and greater staff and student engagement with sustainability! Hello, welcome to the February Green Impact

  4. Biochem. J. (2002) 368, 1721 (Printed in Great Britain) 17 RESEARCH COMMUNICATION

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    2002-01-01

    of green fluorescent protein (`GFP')-like fluorescent proteins (FPs) from species of the class Anthozoa demonstrated superiority of the tandem tag in the in i o INTRODUCTION Green fluorescent protein (GFP) from hypothesize that covalently Abbreviations used: EGFP, enhanced green fluorescent protein; EYFP, enhanced

  5. Replacing Fluorescent Lightbulbs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lightbulbs Replacing Fluorescent Lightbulbs July 29, 2012 - 6:37pm Addthis Although fluorescent lightbulbs are generally energy efficient, you can replace them with...

  6. GREEN INFRASTRUCTURE Researchers

    E-Print Network [OSTI]

    Delaware, University of

    SUPPORTING URBAN GREEN INFRASTRUCTURE Researchers: Jenny Caldwell Catherine Cruz-Ortiz Craig Dsouza are supported at the master's and doctoral levels. #12;Supporting Urban Green Infrastructure Researchers: Jenny's Water Resources Agency, for providing information and guidance in the completion of this research. #12

  7. GREEN MOUNTAIN MORRIS DANCERS

    E-Print Network [OSTI]

    GREEN MOUNTAIN MORRIS DANCERS A young men's team performing Morris & Sword dances from England Mountain (boys) and Maple Leaf (girls) will be recruiting new members in January 2009, typically 6th grade, but as a springtime dance, to awaken the earth. The Green Mountain Morris and Maple Leaf Morris are based in Norwich

  8. Sandia Energy - A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Green Technology Home Energy Research EFRCs Solid-State Lighting Science EFRC A Green Technology A Green TechnologyTara Camacho-Lopez2015-05-11T21:08:32+00:00 Solid-State...

  9. Going green earns Laboratory gold

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Going green earns Laboratory gold Going green earns Laboratory gold The Laboratory's newest facility is its first to achieve both the Leadership in Energy and Environmental Design...

  10. Green Light Pulse Oximeter

    DOE Patents [OSTI]

    Scharf, John Edward (Oldsmar, FL)

    1998-11-03

    A reflectance pulse oximeter that determines oxygen saturation of hemoglobin using two sources of electromagnetic radiation in the green optical region, which provides the maximum reflectance pulsation spectrum. The use of green light allows placement of an oximetry probe at central body sites (e.g., wrist, thigh, abdomen, forehead, scalp, and back). Preferably, the two green light sources alternately emit light at 560 nm and 577 nm, respectively, which gives the biggest difference in hemoglobin extinction coefficients between deoxyhemoglobin, RHb, and oxyhemoglobin, HbO.sub.2.

  11. The Air-Fluorescence Yield

    E-Print Network [OSTI]

    F. Arqueros; F. Blanco; D. Garcia-Pinto; M. Ortiz; J. Rosado

    2008-07-30

    Detection of the air-fluorescence radiation induced by the charged particles of extensive air showers is a well-established technique for the study of ultra-high energy cosmic rays. Fluorescence telescopes provide a nearly calorimetric measure of the primary energy. Presently the main source of systematic uncertainties comes from our limited accuracy in the fluorescence yield, that is, the number of fluorescence photons emitted per unit of energy deposited in the atmosphere by the shower particles. In this paper the current status of our knowledge on the fluorescence yield both experimental an theoretical will be discussed.

  12. Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit

    E-Print Network [OSTI]

    Agmon, Noam

    Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit protein, photo-excitation leads to excited-state proton transfer from its chromophore, leaving behind a strongly fluorescing anion, while the proton is commonly thought to migrate internally to Glu-222. X

  13. Green Colorado Credit Reserve

    Broader source: Energy.gov [DOE]

    The Green Colorado Credit Reserve (GCCR) is a loan loss reserve that was created by the Colorado Energy Office (CEO) to incentivize private lenders in Colorado to make small commercial loans up to ...

  14. Green Chemistry and Workers

    E-Print Network [OSTI]

    2009-01-01

    AND GREEN CHEMISTRY IN THE CLEAN-ENERGY ECONOMY Michael P.rapid development of clean-energy technologies—of the greenof economic growth in clean-energy technologies and energy

  15. Green Supercomputing at Argonne

    ScienceCinema (OSTI)

    Pete Beckman

    2010-01-08

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing?everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently.

  16. USPS – Lean Green Teams

    SciTech Connect (OSTI)

    2012-08-01

    Institutional change case study details the U.S. Postal Service's Lean Green Teams, which collaborate across functions to identify and implement low- and no-cost ways to conserve natural resources, purchase fewer consumable products, and reduce waste.

  17. Green Supercomputing at Argonne

    SciTech Connect (OSTI)

    Pete Beckman

    2009-11-18

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing—everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently.

  18. NY Green Bank

    Broader source: Energy.gov (indexed) [DOE]

    NY Green Bank 1359 Broadway, 19th Floor, New York, NY 10018 212.379.6257 | www.greenbank.ny.gov | info@nygreenbank.ny.gov Monday, October 6 th , 2014 Remarks by Nicholas Whitcombe,...

  19. Green Supercomputing at Argonne

    ScienceCinema (OSTI)

    Beckman, Pete

    2013-04-19

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing?everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently. Argonne was recognized for green computing in the 2009 HPCwire Readers Choice Awards. More at http://www.anl.gov/Media_Center/News/2009/news091117.html Read more about the Argonne Leadership Computing Facility at http://www.alcf.anl.gov/

  20. Modeling Feat Sheds Light on Protein Channel's Function

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    translocon channel (green), which is embedded in the cell membrane (yellow, white). Proteins that are inserted via the ribosome into the channel can either be laterally...

  1. Magnetic fluorescent lamp

    DOE Patents [OSTI]

    Berman, S.M.; Richardson R.W.

    1983-12-29

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  2. Localization of human elav-like neuronal protein 1 (Hel-N1) on chromosome 9p21 by chromosome microdissection polymerase chain reaction and fluorescence in situ hybridization

    SciTech Connect (OSTI)

    Han, Jian; Knops, J.F.; Longshore, J.W.; King, P.H.

    1996-08-15

    Hel-N1 is a member of the highly conserved elav family of neuronal genes. It shares considerable sequence homology with HuD, another human member, and both genes are expressed in brain. HuD was recently mapped to chromosome 1p34. Here, we have utilized chromosome microdissection polymerase chain reaction and fluorescence in situ hybridization to map Hel-N1 to chromosome 9p21. The different chromosomal locations of these homologous genes underscore their distinct identities. 10 refs., 2 figs.

  3. Amplifying Fluorescent Polymers Direct Synthesis of an OligonucleotidePoly-

    E-Print Network [OSTI]

    Tan, Weihong

    Amplifying Fluorescent Polymers Direct Synthesis of an Oligonucleotide­Poly- (phenylene ethynylene bioprobes can be constructed with these polymers for selective target recog- nition. To achieve this objective, the polymer must be conjugated with a biomolecule such as a DNA strand, a peptide, or a protein

  4. Fluorescence analyzer for lignin

    DOE Patents [OSTI]

    Berthold, John W. (Salem, OH); Malito, Michael L. (Hubbard, OH); Jeffers, Larry (Alliance, OH)

    1993-01-01

    A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

  5. Fluorescent temperature sensor

    DOE Patents [OSTI]

    Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

    2009-03-03

    The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

  6. "Technology Wedges" for Implementing Green Manufacturing

    E-Print Network [OSTI]

    Dornfeld, David; Wright, Paul

    2007-01-01

    Integration issues in green design and manufacturing."schematic of the green elements of design and manufacturing1. SCHEMATIC OF “GREEN” ELEMENTS OF DESIGN AND PRODUCTION,

  7. 2012 Green Utility Leaders | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2012 Green Utility Leaders 2012 Green Utility Leaders Ranking the Top Green Power Providers See All Leaders x Renewable Energy Sales Total Customer Participants Customer...

  8. Fluorescent Lighting | Department of Energy

    Office of Environmental Management (EM)

    - by far the most common form of fluorescent lighting but rarely found in residential buildings -- are much more energy efficient than incandescent lamps and are ideally suited...

  9. Green Leasing Deployment Portfolio

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,Executive CompensationEnergyGetDepartment of EnergyCollarGreenGreen

  10. Seeing Green: Speculative Urbanism in the Green Economy

    E-Print Network [OSTI]

    Knuth, Sarah

    2014-01-01

    unconventional green resources – energy efficiency and itschallenge efficiency and energy, resource, or emissions3: Making Energy Efficiency a Resource………………………………..…. ….58

  11. TRENDS in Cell Biology Vol.12 No.9 September 2002 http://tcb.trends.com 0962-8924/02/$ see front matter 2002 Elsevier Science Ltd. All rights reserved. PII: S0962-8924(02)02343-7

    E-Print Network [OSTI]

    Mullen, Sean P.

    Research News The availability of green fluorescent protein (GFP) as a tracer for observing proteins is extremely desirable [1­3]. Green and red fluorescent proteins, additional colour variants and alternative for labelling live cells are described. GFP and GFP colour variants Green fluorescent protein [4

  12. Green Infrastructure for Arid Communities

    Broader source: Energy.gov [DOE]

    On March 24, 2015, from 1:00pm – 2:30pm EDT, EPA's Green Infrastructure Program will launch our 2015 Webcast Series with the webinar Green Infrastructure for Arid Communities. This webinar aims to...

  13. GREEN HOMES LONG ISLAND

    E-Print Network [OSTI]

    Kammen, Daniel M.

    energy bill, reduce your carbon footprint... at little or no cost to you. #12;A Message From Supervisor energy-efficient and reduce our community's carbon footprint. Why do we call it Long Island Green Homes to yourevery day. By making basic improvements to yourevery day home, you can reduce your carbon footprint

  14. Environmental Sustainability & Green Energy

    E-Print Network [OSTI]

    Lennard, William N.

    Environmental Sustainability & Green Energy With escalating concerns about global energy shortages in research related to energy deficits, sustainability and pollution. Biofuels and Alternative EnergyBioproductsInitiative: promoting sustainable farming and renewable energy through a biogas facility and the Institute for

  15. November 2011 Green Bulletin

    E-Print Network [OSTI]

    Ishida, Yuko

    of which contain essential oils or other natural plant extracts targeting weeds. The majority Turf | Page 4 New Online Courses | Page 6 he public's increasing demand for safe "green" products has. Information on the efficacy of these new products is limited. However, environmental awareness groups

  16. Cooking with Green Beans 

    E-Print Network [OSTI]

    Anding, Jenna

    2008-12-09

    need 2 15-ounce cans of green beans, drained 10.5-ounce can cream of mushroom soup ? cup milk 2.75-ounce can fried onion rings, divided in half black pepper (if you like) How to make it 1. Wash...

  17. NIST Presents Green Button: Policy

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01

    Part 2/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  18. NIST Presents Green Button: Technical

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29

    Part 3/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  19. NIST Presents Green Button: Intro

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29

    Part 1/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  20. NIST Presents Green Button: Policy

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29

    Part 2/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  1. NIST Presents Green Button: Technical

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01

    Part 3/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  2. NIST Presents Green Button: Intro

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01

    Part 1/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  3. Manipulating RuBisCO accumulation in the green alga, Chlamydomonas reinhardtii

    E-Print Network [OSTI]

    Manipulating RuBisCO accumulation in the green alga, Chlamydomonas reinhardtii Xenie Johnson in algal mrl1 mutants leads to a complete absence of RuBisCO large subunit protein and thus a lack of accu between green algae (Chlamydomonas X. Johnson (&) Centre National de la Recherche Scientifique, Unite

  4. LED-Induced Fluorescence System for Tea Classification and Quality Assessment

    E-Print Network [OSTI]

    Dong, Yongjiang; Mei, Liang; Feng, Chao; Yan, Chunsheng; He, Sailing

    2013-01-01

    A fluorescence system is developed by using several light emitting diodes (LEDs) with different wavelengths as excitation light sources. The fluorescence detection head consists of multi LED light sources and a multimode fiber for fluorescence collection, where the LEDs and the corresponding filters can be easily chosen to get appropriate excitation wavelengths for different applications. By analyzing fluorescence spectra with the principal component analysis method, the system is utilized in the classification of four types of green tea beverages and two types of black tea beverages. Qualities of the Xihu Longjing tea leaves of different grades, as well as the corresponding liquid tea samples, are studied to further investigate the ability and application of the system in the evaluation of classification/quality of tea and other foods.

  5. RELATED LINKS Green Technology for

    E-Print Network [OSTI]

    , energy efficiency continues to be the primary factor driving the green building movement, squaring Congress considers how to encourage more energy-efficient construction, it must keep affordability in mindRELATED LINKS Green Technology for 2009: See the Photos Green Building: Getting Past the Media Hype

  6. Laser-Stimulated Fluorescence in Paleontology

    E-Print Network [OSTI]

    Kaye, Thomas G.; Falk, Amanda Renee; Pittman, Michael; Sereno, Paul C.; Martin, Larry D.; Burnham, David A.; Gong, Enpu; Xu, Xing; Wang, Yinan

    2015-05-27

    Fluorescence using ultraviolet (UV) light has seen increased use as a tool in paleontology over the last decade. Laser-stimulated fluorescence (LSF) is a next generation technique that is emerging as a way to fluoresce paleontological specimens...

  7. Design strategies for fluorescent biodegradable polymeric biomaterials

    E-Print Network [OSTI]

    Yang, Jian

    Design strategies for fluorescent biodegradable polymeric biomaterials Yi Zhangab and Jian Yang biomaterials: biodegradable fluorescent polymers. Researchers have made significant efforts in developing versatile fluorescent biomaterials due to their promising applications in biological/biomedical labeling

  8. Lumens Placard (Green) | Department of Energy

    Energy Savers [EERE]

    Green) Lumens Placard (Green) PDF icon Lumens: The new way to shop for light More Documents & Publications Lumens Placard (Black...

  9. City of Chicago- Green Power Purchasing

    Broader source: Energy.gov [DOE]

    For more green power purchasing program listings, visit the U.S. Department of Energy Green Power Network.

  10. Ghana Green Building Council public launch

    E-Print Network [OSTI]

    Ghana Green Building Council public launch examples of green buildings in South Africa eric noir, WSP GBD 17 August 2011 #12;Ghana Green Building Council public launch | examples of green buildings in South Africa GREEN by DESIGN PLATINUM GOLD MAJORSPONSORS #12;Ghana Green Building Council public launch

  11. Histone H1 proteins in Chlamydomonas reinhardtii 

    E-Print Network [OSTI]

    Salinger, Andrew Paul

    1994-01-01

    The Hl proteins of the unicellular green alga Chlamydomonas reinhardtii were extracted from isolated nuclei, fractionated by two-dimensional electrophoresis, and analyzed by peptide mapping and N-terminal sequencing. C. reinhardtli histones were...

  12. Nuclear Resonance Fluorescence for Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian

    2010-01-01

    clandestine material with nuclear resonance fluorescence”.E. Norman, UC Berkeley Dept. of Nuclear Engineering, privatepp. 349. G. Warren et al. “Nuclear Resonance Fluorescence of

  13. Design and synthesis of probes for detection of protein-protein interaction and RNA localization

    E-Print Network [OSTI]

    Ryan, Jeremy Adam

    2005-01-01

    The use of the ketone biotin - benzophenone-biotin hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB p50 dimers was assessed. A series of benzophenone-based probes were synthesized and ...

  14. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01

    likelihood of investing in green design or energy efficienton the benefits of green design to commercial buildinginfluence the adoption of green design and energy efficiency

  15. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01

    of green and energy efficient buildings throughout the urbancosts of green and energy efficient buildings, barriers toof green or energy-efficient buildings across a metropolitan

  16. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01

    the larger diffusion of green and energy efficient buildingsowners, the costs of green and energy efficient buildings,market. Demand for Green and Energy Efficient Buildings The

  17. Red phosphors for use in high CRI fluorescent lamps

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

    2005-11-15

    Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

  18. IEEE TRANSACTIONS ON NANOBIOSCIENCE, VOL. 5, NO. 1, MARCH 2006 41 Computational Modeling of a New Fluorescent

    E-Print Network [OSTI]

    IEEE TRANSACTIONS ON NANOBIOSCIENCE, VOL. 5, NO. 1, MARCH 2006 41 Computational Modeling of a New Truong, Member, IEEE Abstract--The class of fluorescence resonance energy transfer (FRET) protein

  19. Nuclear Resonance Fluorescence for Nuclear Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian Joseph

    2010-01-01

    Potential of Nuclear Resonance Fluorescence . . . . . . . .2.9.1 Nuclear ThomsonSections . . . . . . . . . . . . . . . Nuclear Resonance

  20. Mapping of the receptor protein-tyrosine kinase 10 to human chromosome 1q21-q23 and mouse chromosome 1H1-5 by fluorescence in situ hybridization

    SciTech Connect (OSTI)

    Edelhoff, S.; Disteche, C.M.; Lai, C.

    1995-01-01

    Receptor protein-tyrosine kinases (PTKs) play a critical role in the transduction of signals important to cell growth, differentiation, and survival. Mutations affecting the expression of receptor PTK genes have been associated with a number of vertebrate and invertebrate developmental abnormalities, and the aberrant regulation of tyrosine phosphorylation is implicated in a variety of neoplasias. One estimate suggests that approximately 100 receptor PTK genes exist in the mammalian genome, about half of which have been identified. The tyro-10 receptor protein-tyrosine kinase, first identified in a PCR-based survey for novel tyrosine kinases in the rat nervous system, defines a new subfamily of PTKs. It exhibits a catalytic domain most closely related to those found in the trk PTK receptor subfamily, which transduces signals for nerve growth factor and the related molecules brain-derived neurotrophic factor (BDNF), neurotrophin-3, and neurotrophin-4 (NT-3 and NT-4). Trk and the related PTK receptors trkB and trkC play a critical role in the neurotrophin-dependent survival of subsets of sensory and motor neurons. The predicted tyro-10 extracellular region is, however, distinct from that of the trk subfamily and is unique except for a domain shared with the blood coagulation factors V and VIII, thought to be involved in phospholipid binding. Although tyro-10 RNA is most abundant in heart and skeletal muscle in the adult rat, it is expressed in a wide variety of tissues, including the developing and mature brain. Tyro-10 appears identical to the murine TKT sequence reported by Karn et al. and exhibits a high degree of similarity with the CaK, DDR, and Nep PTKs. A ligand for tyro-10 has not yet been identified. 10 refs., 1 fig.

  1. Seeing Green: Speculative Urbanism in the Green Economy

    E-Print Network [OSTI]

    Knuth, Sarah

    2014-01-01

    energy, energy efficiency and building weatherization, andthe advanced energy efficiency-building materials cluster(s)power, energy efficiency, advanced building materials, green

  2. High throughput protein production screening

    DOE Patents [OSTI]

    Beernink, Peter T. (Walnut Creek, CA); Coleman, Matthew A. (Oakland, CA); Segelke, Brent W. (San Ramon, CA)

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  3. Innovative Financing for Green Infrastructure

    Office of Energy Efficiency and Renewable Energy (EERE)

    Topic OverviewFinancing green infrastructure is critical to taking projects from planning to implementation and beyond, including sustaining operations and maintenance. This 90-minute webcast will...

  4. Easy Gardening.....Green Beans 

    E-Print Network [OSTI]

    Cotner, Sam; Parsons, Jerry; Masabni, Joseph

    2009-04-21

    This publication explains all aspects of growing green beans in a home garden. Topics include: site selection, soil preparation, varieties, planting, fertilizing, watering, harvesting, insects, diseases and serving....

  5. Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo

    SciTech Connect (OSTI)

    Kim, Eun-Sung; Yang, Seung-Woo; Hong, Dong-Ki; Kim, Woo-Taek; Kim, Ho-Guen; Lee, Sang-Kyou

    2010-01-29

    Non-viral gene delivery is a safe and suitable alternative to viral vector-mediated delivery to overcome the immunogenicity and tumorigenesis associated with viral vectors. Using the novel, human-origin Hph-1 protein transduction domain that can facilitate the transduction of protein into cells, we developed a new strategy to deliver naked DNA in vitro and in vivo. The new DNA delivery system contains Hph-1-GAL4 DNA-binding domain (DBD) fusion protein and enhanced green fluorescent protein (EGFP) reporter plasmid that includes the five repeats of GAL4 upstream activating sequence (UAS). Hph-1-GAL4-DBD protein formed complex with plasmid DNA through the specific interaction between GAL4-DBD and UAS, and delivered into the cells via the Hph-1-PTD. The pEGFP DNA was successfully delivered by the Hph-1-GAL4 system, and the EGFP was effectively expressed in mammalian cells such as HeLa and Jurkat, as well as in Bright Yellow-2 (BY-2) plant cells. When 10 {mu}g of pEGFP DNA was intranasally administered to mice using Hph-1-GAL4 protein, a high level of EGFP expression was detected throughout the lung tissue for 7 days. These results suggest that an Hph-1-PTD-mediated DNA delivery strategy may be an useful non-viral DNA delivery system for gene therapy and DNA vaccines.

  6. Inside this issue: Green Dorm,

    E-Print Network [OSTI]

    Alexandrova, Ivana

    Inside this issue: Energy Campaign 1 On Campus: Green Dorm, CFL Exchange, LLC 2 - 3 Around Town, UAlbany Outdoors 7 Project Green Challenge 8 Opportunities 9 Get Involved 10 Upcoming Events 11 September usage by 9%. Just shy of our goal of 10%. This year we hope to meet that goal! We've completed two weeks

  7. GREEN GUIDE MAISON AM GROND

    E-Print Network [OSTI]

    van der Torre, Leon

    For this task, we took the ideas from Zihomara Matar. Author of the ECO2 practice guides. Task 4 ­ Laundry: GoodGREEN GUIDE MAISON AM GROND One Earth... Once Chance Team Members: Jose Miguel Lopez Becerra ­ Green Guide Task 1 ­ Garbage Separation This task is related to explain the different types of garbage

  8. Going for "Green" Sustainable Building

    E-Print Network [OSTI]

    Going for "Green" Sustainable Building Certification Statistics Europe Status May 2011 rics.org/sustainability #12;Sustainable Building Certification Statistics Europe Foreword Investors are increasingly the usefulness of sustainability certificates in mainstreaming the uptake of so-called `green' buildings across

  9. greenhouse gas inve green developmen

    E-Print Network [OSTI]

    Collins, Gary S.

    greenhouse gas inve green developmen energy conservation transportation carbon offs student facult;greenhouse gas inventory green development energy conservation transportation carbon offsets student faculty. Changi natural gas as a primary fuel allowed us to find cleaner and more effici university. Both in 1988

  10. Douglas H. Green Piper & Marbury

    E-Print Network [OSTI]

    Douglas H. Green Piper & Marbury 1200 Nineteenth St., N.W. Washington, D.C., 20036-2430 [Date Signed: April 7, 1997] Re: Reproposal of HWIR Waste Rule and Commercial Mixed Waste Dear Mr. Green waste" refers to low-level radioactive hazardous mixed wastes generated by nuclear power plants where

  11. BRANCHED ALKANES FROM BLUE-GREEN ALGAE

    E-Print Network [OSTI]

    Han, Jerry; Calvin, Melvin.

    2008-01-01

    ALKANES FROM BLUE-GREEN ALGAE RECEIV r -· LAWREW RADIATIONAlkanes From Blue-Green Algae by Jerry Han and Oep~rtment l~alkanes from blue-green algae were separated on a The

  12. Green Tea and Its Antioxidant Properties

    E-Print Network [OSTI]

    Yeh, Jason

    1999-01-01

    of effects of drinking green tea on cardiovascular and liverWeststrate JA. Effects of green tea, black tea and dietaryof low-density lipoproteins by green tea antioxidants. FASEB

  13. Energy Department Wins EPA Green Power Award

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Environmental Protection Agency honored the Energy Department with the Green Power Leadership Award, recognizing that DOE is one of America’s top green power consumers while also being a leader in advancing the green power market.

  14. GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS

    E-Print Network [OSTI]

    Shoj, Toshiaki

    P describe Green functions QO(u)* *). Note that the matrix is completely determined GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS University of Tokyo Noda, Chiba 278-8510, Japan Abstract. Green functions

  15. CHANCELLOR'S SUSTAINABILITY COMMITTEE GREEN BUILDING SUBCOMMITTEE

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    CHANCELLOR'S SUSTAINABILITY COMMITTEE GREEN BUILDING SUBCOMMITTEE UNIVERSITY OF MASSACHUSETTS AMHERST FALL 2013 GBGBGUIDELINES 2013 UPDATE #12;#12;Theseguidelineshavebeen prepared by the Green, policies and practices as they pertain to green building and LEED certification. It is our hope

  16. Concentration dependence of fluorescence signal in a microfluidic fluorescence detector

    E-Print Network [OSTI]

    Klotzkin, David

    ] demonstrated a compact device using an OLED as light source and an integrated p-i-n photodiode as detector Available online 6 February 2010 Keywords: Lab-on-a-chip On-chip fluorescence Cross-polarization OLED OPD et al. [3] have recently demonstrated the use of a polyfluorene based pLED (polymer LED) as light

  17. Green Libraries Are More Than Just Buildings

    E-Print Network [OSTI]

    Aulisio, George J.

    2013-01-01

    and measurable green building design, construction,Green Building Council (USGBC) certification that stands for Leadership in Energy and Environmental Design.

  18. The Green Cathedral: Sustainable Development of Amazonia

    E-Print Network [OSTI]

    Tobin, R. James

    1994-01-01

    The Green Cathedral: Sustainable Development of Amazonia R.The Green Cathedral: Sustainable Development of Amazonia. (Ais between sustainable and unsustainable development of the

  19. Energy Innovation: Green Button Initiative Empowering Americans...

    Energy Savers [EERE]

    Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money...

  20. Energy Department Issues Green Building Certification System...

    Office of Environmental Management (EM)

    Issues Green Building Certification System Final Rule to Support Increased Energy Measurement and Efficient Building Design Energy Department Issues Green Building Certification...

  1. Greening up fossil fuels with carbon sequestration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Greening up fossil fuels with carbon sequestration 1663 Los Alamos science and technology magazine Latest Issue:October 2015 past issues All Issues submit Greening up fossil...

  2. Greening Up” Cross-Coupling Chemistry

    E-Print Network [OSTI]

    Lipshutz, Bruce H.; Abela, Alexander R.; Boškovi?, Žarko V.; Nishikata, Takashi; Duplais, Christophe; Krasovskiy, Arkady

    2010-01-01

    today. Insofar as green chemistry is concerned, however,Handbook of organopalladium chemistry for organic synthesis.Hanefeld U (2007) Green chemistry and catalysis. Wiley-VCH,

  3. Green Tea and Its Antioxidant Properties

    E-Print Network [OSTI]

    Yeh, Jason

    1999-01-01

    of effects of drinking green tea on cardiovascular and liverthe epidemiological benefits of tea drinking. FEBS Letters.Weststrate JA. Effects of green tea, black tea and dietary

  4. Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement

    E-Print Network [OSTI]

    Cunningham, Brian

    to vertical radiation using a two-dimensional photonic crystal in a semiconductor light-emitting diode," Appl light-emitting diode," IEEE J. Quantum Electron. 36, 1131-1144 (2000). 3. S. Pillai, K. R. Catchpole, T. Trupke, G. Zhang, J. Zhao, and M. A. Green, "Enhanced emission from Si- based light-emitting diodes using

  5. Capacitive sensing with a fluorescent lamp

    E-Print Network [OSTI]

    Cooley, John Jacob

    2007-01-01

    This work presents a modified fluorescent lamp that can be used as a capacitive sensing system. The lamp sensor measures changes in the electric fields emitted from the fluorescent bulbs in order to deduce the presence and ...

  6. Covered Product Category: Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  7. Covered Product Category: Fluorescent Ballasts

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and federal efficiency requirements across a variety of product categories, including fluorescent ballasts, which is a FEMP designated product category. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  8. Green roofs: potential at LANL

    SciTech Connect (OSTI)

    Pacheco, Elena M [Los Alamos National Laboratory

    2009-01-01

    Green roofs, roof systems that support vegetation, are rapidly becoming one of the most popular sustainable methods to combat urban environmental problems in North America. An extensive list of literature has been published in the past three decades recording the ecological benefits of green roofs; and now those benefits have been measured in enumerated data as a means to analyze the costs and returns of green roof technology. Most recently several studies have made substantial progress quantifying the monetary savings associated with storm water mitigation, the lessoning of the Urban Heat Island, and reduction of building cooling demands due to the implementation of green roof systems. Like any natural vegetation, a green roof is capable of absorbing the precipitation that falls on it. This capability has shown to significantly decrease the amount of storm water runoff produced by buildings as well as slow the rate at which runoff is dispensed. As a result of this reduction in volume and velocity, storm drains and sewage systems are relieved of any excess stress they might experience in a storm. For many municipalities and private building owners, any increase in storm water mitigation can result in major tax incentives and revenue that does not have to be spent on extra water treatments. Along with absorption of water, vegetation on green roofs is also capable of transpiration, the process by which moisture is evaporated into the air to cool ambient temperatures. This natural process aims to minimize the Urban Heat Island Effect, a phenomenon brought on by the dark and paved surfaces that increases air temperatures in urban cores. As the sun distributes solar radiation over a city's area, dark surfaces such as bitumen rooftops absorb solar rays and their heat. That heat is later released during the evening hours and the ambient temperatures do not cool as they normally would, creating an island of constant heat. Such excessively high temperatures induce heat strokes, heat exhaustion, and pollution that can agitate the respiratory system. The most significant savings associated with green roofs is in the reduction of cooling demands due to the green roof's thermal mass and their insulating properties. Unlike a conventional roof system, a green roof does not absorb solar radiation and transfer that heat into the interior of a building. Instead the vegetation acts as a shade barrier and stabilizes the roof temperature so that interior temperatures remain comfortable for the occupants. Consequently there is less of a demand for air conditioning, and thus less money spent on energy. At LANL the potential of green roof systems has already been realized with the construction of the accessible green roof on the Otowi building. To further explore the possibilities and prospective benefits of green roofs though, the initial capital costs must be invested. Three buildings, TA-03-1698, TA-03-0502, and TA-53-0031 have all been identified as sound candidates for a green roof retrofit project. It is recommended that LANL proceed with further analysis of these projects and implementation of the green roofs. Furthermore, it is recommended that an urban forestry program be initiated to provide supplemental support to the environmental goals of green roofs. The obstacles barring green roof construction are most often budgetary and structural concerns. Given proper resources, however, the engineers and design professionals at LANL would surely succeed in the proper implementation of green roof systems so as to optimize their ecological and monetary benefits for the entire organization.

  9. Characterization of Sol-gel Encapsulated Proteins using Small-angle Neutron Scattering

    SciTech Connect (OSTI)

    Luo, Guangming [ORNL; Zhang, Qiu [ORNL; Del Castillo, Alexis Rae [ORNL; Urban, Volker S [ORNL; O'Neill, Hugh Michael [ORNL

    2009-01-01

    Entrapment of biomolecules in silica-derived sol-gels has grown into a vibrant area of research since it was originally demonstrated. However, accessing the consequences of entrapment on biomolecules and the gel structure remains a major challenge in characterizing these biohybrid materials. We present the first demonstration that it is possible with small-angle neutron scattering (SANS) to study the conformation of dilute proteins that are entrapped in transparent and dense sol-gels. Using deuterium-labeled green fluorescent protein (GFP) and SANS with contrast variation, we demonstrate that the scattering signatures of the sol-gel and the protein can be separated. Analysis of the scattering curves of the sol-gels using a mass-fractal model shows that the size of the colloidal silica particles and the fractal dimensions of the gels were similar in the absence and presence of protein, demonstrating that GFP did not influence the reaction pathway for the formation of the gel. The major structural difference in the gels was that the pore size was increased 2-fold in the presence of the protein. At the contrast match point for silica, the scattering signal from GFP inside the gel became distinguishable over a wide q range. Simulated scattering curves representing a monomer, end-to-end dimer, and parallel dimer of the protein were calculated and compared to the experimental data. Our results show that the most likely structure of GFP is that of an end-to-end dimer. This approach can be readily applied and holds great potential for the structural characterization of complex biohybrid and other materials.

  10. Identifying Modulators of Protein-Protein Interactions Using Photonic Crystal Biosensors James T. Heeres

    E-Print Network [OSTI]

    Cunningham, Brian

    Identifying Modulators of Protein-Protein Interactions Using Photonic Crystal Biosensors James T or untagged version of the cognate partner. This assay, based on photonic crystal (PC) biosensor technology or fluorescence measurements. PC biosensors are surface structures comprised of a subwavelength polymer grating

  11. GREEN BUILDINGS IN CHALLENGES AND

    E-Print Network [OSTI]

    Delaware, University of

    science, economics, sociology, geography, philosophy, urban planning, environmental studies, history justice, conservation and renewable energy options, integrated resource planning, and technologyGREEN BUILDINGS IN DELAWARE: CHALLENGES AND OPPORTUNITIES FINAL REPORT A Renewable Energy

  12. Green Building- Efficient Life Cycle 

    E-Print Network [OSTI]

    Kohns, R.

    2008-01-01

    the components “Sustainable Building Design”, “Life Cycle Cost Analysis”, “Green Building Certification” and “Natural Resources Management”. These components are deliberately arranged around the life cycle of the real estate concerned. This allows a different...

  13. Broward County- Green Building Policy

    Broader source: Energy.gov [DOE]

    In October 2008, Board of County Commissioners of Broward County passed a resolution creating the County Green Building Policy. All new County-owned and operated buildings must achieve a minimum...

  14. Mr. Bobby L. Green Texas Tech University

    E-Print Network [OSTI]

    Chen, Xinzhong

    ., Tanju, B. T., Gransberg, D. D., Green, B., Gransberg, N. (1998). Design a Luminance, Illuminance Using CCD Video Imaging Technology. Integrated Design and Process technology, 5. Slowik, T. J., Green, BMr. Bobby L. Green Texas Tech University (806) 742-3538 bobby.green@ttu.edu Education and Post

  15. Lean Green Skating Machine Report No. 1

    E-Print Network [OSTI]

    Levi, Anthony F. J.

    and trying it for him/herself. The Lean Green Skating Machine is designed to help skaters recognize when Machine Report No. 1 Summary This report includes the details and design of the Lean Green Skating MachineLean Green Skating Machine Report No. 1 Lean Green Skating Machine Report No. 1 Submitted by Simon

  16. Green Data Centers: The European Sky

    E-Print Network [OSTI]

    Lefèvre, Laurent

    jobs. Energy Efficiency Plan 2011 Energy Efficiency Directive #12;1st Generation FIT4Green of resources (on/off) Cooperation between Data Centers GAMES: Green Active Management of Energy in IT Service)les Iteration #12;: GreenSDA & GreenSLA Active collaboration in data centre ecosystem to reduce energy

  17. for Students, Sta & Visitors Green Guide

    E-Print Network [OSTI]

    Aickelin, Uwe

    - Recycling Guide - Water & Pollution - Green Shopping - Saving Energy Together - Sustainable Travel Pleasefor Students, Sta & Visitors Green Guide Greener Swansea University-Only if we are Green #12;Welcome ......to our new Green Guide. As students, sta or visitors we under- stand that our activities

  18. Guide to Purchasing Green Power

    Broader source: Energy.gov [DOE]

    The Guide to Purchasing Green Power is intended for organizations that are considering the merits of buying green power as well as those that have decided to buy it and want help doing so. The guide was written for a broad audience, including businesses, government agencies, universities, and all organizations wanting to diversify their energy supply and reduce the environmental impact of their electricity use. The Guide can help with planning an on-site renewable generation project.

  19. Minimal models for proteins and RNA: From folding to function

    E-Print Network [OSTI]

    D. L. Pincus; S. S. Cho; C. Hyeon; D. Thirumalai

    2008-08-22

    We present a panoramic view of the utility of coarse-grained (CG) models to study folding and functions of proteins and RNA. Drawing largely on the methods developed in our group over the last twenty years, we describe a number of key applications ranging from folding of proteins with disulfide bonds to functions of molecular machines. After presenting the theoretical basis that justifies the use of CG models, we explore the biophysical basis for the emergence of a finite number of folds from lattice models. The lattice model simulations of approach to the folded state show that non-native interactions are relevant only early in the folding process - a finding that rationalizes the success of structure-based models that emphasize native interactions. Applications of off-lattice $C_{\\alpha}$ and models that explicitly consider side chains ($C_{\\alpha}$-SCM) to folding of $\\beta$-hairpin and effects of macromolecular crowding are briefly discussed. Successful application of a new class of off-lattice model, referred to as the Self-Organized Polymer (SOP), is shown by describing the response of Green Fluorescent Protein (GFP) to mechanical force. The utility of the SOP model is further illustrated by applications that clarify the functions of the chaperonin GroEL and motion of the molecular motor kinesin. We also present two distinct models for RNA, namely, the Three Site Interaction (TIS) model and the SOP model, that probe forced unfolding and force quench refolding of a simple hairpin and {\\it Azoarcus} ribozyme. The predictions based on the SOP model show that force-induced unfolding pathways of the ribozyme can be dramatically changed by varying the loading rate. We conclude with a discussion of future prospects for the use of coarse-grained models in addressing problems of outstanding interest in biology.

  20. Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells

    SciTech Connect (OSTI)

    Chen, Fanqing; Gerion, Daniele

    2004-06-14

    One of the biggest challenges in cell biology is the imaging of living cells. For this purpose, the most commonly used visualization tool is fluorescent markers. However, conventional labels, such as organic fluorescent dyes or green fluorescent proteins (GFP), lack the photostability to allow the tracking of cellular events that happen over minutes to days. In addition, they are either toxic to cells (dyes), or difficult to construct and manipulate (GFP). We report here the use of a new class of fluorescent labels, silanized CdSe/ZnS nanocrystal-peptide conjugates, for imaging the nuclei of living cells. CdSe/ZnS nanocrystals, or so called quantum dots (qdots), are extremely photostable, and have been used extensively in cellular imaging of fixed cells. However, most of the studies about living cells so far have been concerned only with particle entry into the cytoplasm or the localization of receptors on the cell membrane. Specific targeting of qdots to the nucleus of living cells ha s not been reported in previous studies, due to the lack of a targeting mechanism and proper particle size. Here we demonstrate for the first time the construction of a CdSe/ZnS nanocrystal-peptide conjugate that carries the SV40 large T antigen nuclear localization signal (NLS), and the transfection of the complex into living cells. By a novel adaptation of commonly used cell transfection techniques for qdots, we were able to introduce and retain the NLS-qdots conjugate in living cells for up to a week without detectable negative cellular effects. Moreover, we can visualize the movement of the CdSe/ZnS nanocrystal-peptide conjugates from cytoplasm to the nucleus, and the accumulation of the complex in the cell nucleus, over a long observation time period. This report opens the door for using qdots to visualize long-term biological events that happen in the cell nucleus, and provides a new nontoxic, long-term imaging platform for cell nuclear processes.

  1. The Greening of Government: A Study of How Governments Define the Green Agenda

    E-Print Network [OSTI]

    The Greening of Government: A Study of How Governments Define the Green Agenda Executive Summary Institute for Electronic Government, IBM Corporation Page 2 This page is intentionally blank. In the green Corporation Page 3 Foreword What are governments doing to `green' themselves? How do they define their green

  2. Fluorescence based chemical sensors for corrosion detection

    SciTech Connect (OSTI)

    Johnson, R.E.; Agarwala, V.S.

    1997-12-01

    Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.

  3. Assaying protein import into mitochondria using fluorescence spectroscopy 

    E-Print Network [OSTI]

    Cargill, Holly Beth

    2006-08-16

    would like to thank my ever-supportive family: my parents, Nathan and Karen; and my ?boys?, Edward, Ethan, and Caleb. vii TABLE OF CONTENTS Page ABSTRACT... preprotein across the IM requires successive rounds of ATP-hydrolysis, assisted by Pam16, Pam18, and Mge1. Pam18, being a member of the J domain family, stimulates the ATPase activity of mtHsp70, while Pam16 helps to recruit Pam18 to the TIM23 complex...

  4. Mapping protein collapse with single molecule fluorescence and kinetic

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journalspectroscopy ofArticle)SciTechNorrisAlpha Racetrack,synchrotron radiation

  5. Mapping protein collapse with single molecule fluorescence and kinetic

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journalspectroscopy ofArticle)SciTechNorrisAlpha Racetrack,synchrotron

  6. Fluorescence Stopped-flow Studies of Single Turnover Kinetics of E. coli RecBCD Helicase-

    E-Print Network [OSTI]

    Lohman, Timothy M.

    reserved. Keywords: FRET; helicase; motor; recombination; fluorescence*Corresponding author Introduction DNA helicases are motor proteins that use nucleoside triphosphate (NTP) binding and hydrolysis; NLLS, non-linear least squares; b-ME, b-mercapto- ethanol. doi:10.1016/j.jmb.2004.04.009 J. Mol. Biol

  7. Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging

    E-Print Network [OSTI]

    White, Katharine Alice

    2012-01-01

    Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. ...

  8. Analytical approaches to photobiological hydrogen production in unicellular green algae

    E-Print Network [OSTI]

    Hemschemeier, Anja; Melis, Anastasios; Happe, Thomas

    2009-01-01

    monas reinhardtii (green alga). Planta 214:552–561. doi:adaptation in the green alga Chlamydomonas reinhardtii. Eurhydrogenase from the green alga Chlamydomonas reinhardtii.

  9. Occupant satisfaction with indoor environmental quality in green buildings

    E-Print Network [OSTI]

    Abbaszadeh, S.; Zagreus, Leah; Lehrer, D.; Huizenga, C

    2006-01-01

    While it has brought green design and construction practicesand Environmental Design (LEED) Green Building Rating Systemof sustainable design, we question how green buildings are

  10. Skinny Streets and Green Neighborhoods: Design for Environment and Community

    E-Print Network [OSTI]

    Piselli, Kathy

    2006-01-01

    Streets and Green Neighborhoods: Design for Environment andStreets and Green Neighborhoods: Design for Environment anddesign. All make decisions about ratio of gray to green. In

  11. The Green Library Movement: An Overview and Beyond

    E-Print Network [OSTI]

    Antonelli, Monika

    2008-01-01

    the cutting edge of green design. The article “Public InputLibrary. (2008). Green Library Design has goal of Platinum2008). Interest in green library design intensified in

  12. Attachment 1: Green Lease Policies and Procedures for Lease Acquisitio...

    Energy Savers [EERE]

    Attachment 1: Green Lease Policies and Procedures for Lease Acquisition Attachment 1: Green Lease Policies and Procedures for Lease Acquisition PDF icon Green Lease Policies and...

  13. Review: Greening Citizenship: Sustainable Development, the State and Ideology

    E-Print Network [OSTI]

    Laberge, Yves

    2014-01-01

    Greening Citizenship: Sustainable Development, the State andGreening Citizenship: Sustainable Development, the State andGreening Citizenship: Sustainable Development, the State and

  14. Analytical approaches to photobiological hydrogen production in unicellular green algae

    E-Print Network [OSTI]

    Hemschemeier, Anja; Melis, Anastasios; Happe, Thomas

    2009-01-01

    E, Melis A (2000) Microalgae: a green source of renewable Hmany species of green microalgae. Moreover, it has attractedutili- zation of green microalgae and the process of

  15. SEP Success Story: "Green Launching Pad" Supports Clean Energy...

    Energy Savers [EERE]

    "Green Launching Pad" Supports Clean Energy Small Businesses SEP Success Story: "Green Launching Pad" Supports Clean Energy Small Businesses May 24, 2012 - 5:10pm Addthis Green...

  16. Attachment 1: Green Lease Policies and Procedures for Lease Acquisitio...

    Energy Savers [EERE]

    1: Green Lease Policies and Procedures for Lease Acquisition Attachment 1: Green Lease Policies and Procedures for Lease Acquisition PDF icon Green Lease Policies and Procedures...

  17. Community Power Works: Good Jobs, Green Jobs Conference | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Works: Good Jobs, Green Jobs Conference Community Power Works: Good Jobs, Green Jobs Conference A presentation given by Community Power Works at the Good Jobs, Green Jobs...

  18. ENERGY STAR Webinar: ENERGY STAR and Green Building Rating Systems...

    Broader source: Energy.gov (indexed) [DOE]

    resources to help meet requirements for green building rating systems, such as the U.S. Green Building Council's Leadership in Energy and Environmental Design (LEED), the Green...

  19. California Households’ Willingness to Pay for “Green” Electronics

    E-Print Network [OSTI]

    Saphores, Jean-Daniel M; Nixon, Hilary; Ogunseitan, Oladele; Shapiro, Andrew A

    2007-01-01

    Consumer demand for ‘green power’ and energy efficiencyresidential demand for green power. Energy Policy, 29, pp.specific approach to “green” power retailing: Students as

  20. Status Prospects of Green Data Center | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Status Prospects of Green Data Center Status Prospects of Green Data Center Information on energy-saving technology research of green data centers and the potential impacts....

  1. California Households’ Willingness to Pay for “Green” Electronics

    E-Print Network [OSTI]

    Saphores, Jean-Daniel M; Nixon, Hilary; Ogunseitan, Oladele; Shapiro, Andrew A

    2007-01-01

    demand for ‘green power’ and energy efficiency Energyparticipating in “green” energy programs for businesses.residential demand for green power. Energy Policy, 29, pp.

  2. Top 20 Ways to Go Green at Work (and Save the University Money!) Office Depot's three environmental aspirations are to "Buy Green," "Be Green" and

    E-Print Network [OSTI]

    Borenstein, Elhanan

    Top 20 Ways to Go Green at Work (and Save the University Money!) Office Depot's three environmental aspirations are to "Buy Green," "Be Green" and "Sell Green." · By "Buying Green," we have achieved the widest "green product assortment" in the office products industry - approximately 3,500 products with recycled

  3. Green Schools Project Final Report

    SciTech Connect (OSTI)

    Verdict, M.

    2000-09-27

    The Alliance to Save Energy has responded to interest in the Green Schools concept from the New England states of Maine, New Hampshire and Vermont. The Alliance conducted a train-the-trainers workshop in Augusta, Maine March 17--18, 1999. This work is part of a Green Schools replication project leveraged by funds from another source, NORDAX, which contributed $80,000 to provide partial support to staff at the Maine Energy Education Project (MEEP), Vermont Energy Education Program (VEEP), and New Hampshire Governor's Office to develop Green Schools Projects. DOE funds were used to conduct training, develop a network of state and local government, business and school partners to support school efficiency activities in those three states.

  4. Blue-green upconversion laser

    DOE Patents [OSTI]

    Nguyen, Dinh C. (Los Alamos, NM); Faulkner, George E. (Los Alamos, NM)

    1990-01-01

    A blue-green laser (450-550 nm) uses a host crystal doped with Tm.sup.3+. The Tm.sup.+ is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP.

  5. Blue-green upconversion laser

    DOE Patents [OSTI]

    Nguyen, D.C.; Faulkner, G.E.

    1990-08-14

    A blue-green laser (450--550 nm) uses a host crystal doped with Tm[sup 3+]. The Tm[sup 3+] is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP. 3 figs.

  6. Green GECCO | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreen ButtonGreen Fuels

  7. Green Shakti | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreenHighlandLionGreenShakti

  8. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, Daniel L. (Albuquerque, NM); Tangyunyong, Paiboon (Albuquerque, NM)

    1998-01-01

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC.

  9. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, D.L.; Tangyunyong, P.

    1998-01-06

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC. 1 fig.

  10. GFP variants for multispectral imaging of living cells. Jim Haseloff

    E-Print Network [OSTI]

    Franks, Robert

    light, and the accessory protein, GFP, which accepts energy from aequorin and re-emits it as green light the jellyfish green fluorescent protein for use as a directly visible gene marker in Arabidopsis, and developed. Green fluorescent protein markers Marker genes have proved extremely useful for reporting gene

  11. Portable spotter for fluorescent contaminants on surfaces

    DOE Patents [OSTI]

    Schuresko, Daniel D. (Oak Ridge, TN)

    1980-01-01

    A portable fluorescence-based spotter for polynuclear aromatic hydrocarbon contamination on personnel and work area surfaces under ambient lighting conditions is provided. This instrument employs beam modulation and phase sensitive detection for discriminating between fluorescence from organic materials from reflected background light and inorganic fluorescent material. The device uses excitation and emission filters to provide differentiation between classes of aromatic organic compounds. Certain inorganic fluorescent materials, including heavy metal compounds, may also be distinguished from the organic compounds, despite both having similar optical properties.

  12. LED Replacements for Linear Fluorescent Lamps Webcast

    Broader source: Energy.gov [DOE]

    In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting...

  13. Utility Green Pricing Programs: Design, Implementation, and

    E-Print Network [OSTI]

    Utility Green Pricing Programs: Design, Implementation, and Consumer Response February 2004 · NREL-35618 Utility Green Pricing Programs: Design, Implementation, and Consumer Response Lori Bird, Blair Swezey legal liability or responsibility for the accuracy, completeness, or usefulness of any information

  14. Ramblewood Green: Order (2014-CE-23017)

    Broader source: Energy.gov [DOE]

    DOE ordered Ramblewood Green Ltd. to pay a $8,000 civil penalty after finding Ramblewood Green had failed to certify that certain models of cooking products comply with the applicable energy conservation standards.

  15. Greening existing buildings with LEED-EB!

    E-Print Network [OSTI]

    Dirksen, Tyson H

    2008-01-01

    The market of existing office buildings is going green. While early adopters of green buildings were owner-occupiers, there is a current wave of nonowner-occupied office buildings seeking Leadership in Energy and Environmental ...

  16. Green Systems Solar Hot Water

    E-Print Network [OSTI]

    Schladow, S. Geoffrey

    Green Systems Solar Hot Water Heating the Building Co-generation: Heat Recovery System: Solar Thermal Panels (Trex enclosure) Hot Water Storage Tank (TS-5; basement) Hot Water Heaters (HW-1,2; basement) Pre-heats water so water heaters don't need to use as much energy Gas-powered, high efficiency

  17. A Green Laser Pointer Hazard

    E-Print Network [OSTI]

    Jemellie Galang; Allesandro Restelli; Edward W. Hagley; Charles W. Clark

    2010-08-09

    An inexpensive green laser pointer was found to emit 20 mW of infrared radiation during normal use. This is potentially a serious hazard that would not be noticed by most users of such pointers. We find that this infrared emission derives from the design of the pointer, and describe a simple method of testing for infrared emissions using common household items.

  18. Introduction to Green Building & LEED

    E-Print Network [OSTI]

    Zaferatos, Nicholas C.

    Introduction to Green Building & LEED Alistair Jackson Principal O'Brien & Company alistair Housing · Whatcom County Courthouse - LEED for Existing Buildings · Art & Children's Museum · Waterfront · Whatcom Educational Credit Union · Bisese Dental Clinic · Community Food Co-op · Gaston Bay Building #12

  19. Matthew H. Green Lab Address

    E-Print Network [OSTI]

    MacIver, Malcolm A.

    neurophysiology and behavior · Presently working to build virtual reality systems for integrative behavioral cell recordings · Developed an automated tracking algorithm for analysis of behavior using high in larval zebrafish. Society for Integrative and Comparative Biology, Charleston, SC. [Poster] Green, M. H

  20. CHEVROLET | ELECTRIC | GREEN | SPARK EV | TECHNOLOGY. INNOVATION...

    Open Energy Info (EERE)

    CHEVROLET | ELECTRIC | GREEN | SPARK EV | TECHNOLOGY. INNOVATION & SOLUTIONS | GREENER VEHICLES Home There are currently no posts in this category. Syndicate...

  1. UAF Green Department Certification UAF OFFICE OF SUSTAINABILITY

    E-Print Network [OSTI]

    Hartman, Chris

    /Documentation: Computers are set to energy saving modes. Description/Documentation: GREEN DEPARTMENT PLEDGE UAF Green Department Certification UAF OFFICE OF SUSTAINABILITY GREEN DEPARTMENT CERTIFICATION INSTRUCTIONS & FORMS PART I: GETTING STARTED & REQUIRED ACTIONS #12; UAF Green Department Certification

  2. Motivation Green's functions The GW Approximation The Bethe-Salpeter Equation Introduction to Green's functions

    E-Print Network [OSTI]

    Botti, Silvana

    Motivation Green's functions The GW Approximation The Bethe-Salpeter Equation Introduction to Green's functions Matteo Gatti ETSF Users' Meeting and Training Day Ecole Polytechnique - 22 October 2010 #12;bg=whiteMotivation Green's functions The GW Approximation The Bethe-Salpeter Equation Outline 1 Motivation 2 Green

  3. UbiGreen: Investigating a Mobile Tool for Tracking and Supporting Green Transportation Habits

    E-Print Network [OSTI]

    Anderson, Richard

    green transportation behavior, and reactions to early mobile phone "green" application design concepts activity, a visual design capable of engaging users in the goal of increasing green transportation, and the results of our studies, which have implications for the design of future green applications. Author

  4. Grain Fish Money Financing Africa's Green and Blue Revolutions Financing Africa's Green and Blue Revolutions

    E-Print Network [OSTI]

    Grain Fish Money Financing Africa's Green and Blue Revolutions 1 Financing Africa's Green and Blue Revolutions GRAIN FISH MONEY AFRICA PROGRESS REPORT 2014 #12;AFRICA PROGRESS REPORT 2014 2 #12;Grain Fish Money Financing Africa's Green and Blue Revolutions 3 #12;#12;Grain Fish Money Financing Africa's Green

  5. Green Wall Technologies Objective: Compile a comprehensive and up-to-date assessment of green wall

    E-Print Network [OSTI]

    Wolberg, George

    Green Wall Technologies Objective: Compile a comprehensive and up-to-date assessment of green wall technologies that can be used to jump-start design and engineering proposals for green walls in a variety of urban conditions. Background: Green walls--otherwise referred to as living walls or biowalls

  6. GreenPar: Scheduling Parallel High Performance Applications in Green Datacenters

    E-Print Network [OSTI]

    Bianchini, Ricardo

    applications in datacenters partially powered by on-site generation of renewable ("green") energy. GreenPar schedules the workload to maximize the green energy con- sumption and minimize the grid ("brown") energy consump- tion, while respecting a performance service-level agreement (SLA). When green energy

  7. GreenHadoop: Leveraging Green Energy in Data-Processing Frameworks

    E-Print Network [OSTI]

    Bianchini, Ricardo

    GreenHadoop: Leveraging Green Energy in Data-Processing Frameworks ´I~nigo Goiri Dept. of Computer that energy demand and supply must be matched, if we are to take full advantage of the green en- ergy workload to match the green energy supply. In particular, we consider data-processing frameworks, in which

  8. Pluricomplex Green function, pluricomplex Poisson kernel and

    E-Print Network [OSTI]

    Bracci, Filippo

    resem- bles completely the one dimensional equation giving rise to the Green function. More or lessPluricomplex Green function, pluricomplex Poisson kernel and applications FILIPPO BRACCI fbracci@mat.uniroma2.it Abstract. This is a small survey about the pluricomplex Green function of M

  9. GREEN IT STRATEGY WHITE PAPER BIG DATA.

    E-Print Network [OSTI]

    Chou, James

    GREEN IT STRATEGY WHITE PAPER BIG DATA. BIG ENERGY. #12;GREEN:ons in MassachuseUs. In one day, Odyssey's cloud compu:ng power can process the same amount of 250 more kilowaUs annually. HARVARD'S GREEN IT COMMITMENT Achieve University

  10. Dynamic Spectrum Management for Green DSL

    E-Print Network [OSTI]

    Chiang, Mung

    to as `green DSL'. Using this `green DSL' approach, we quantify the power-rate trade-off for different1 Dynamic Spectrum Management for Green DSL Paschalis Tsiaflakisa,, Yung Yib, Mung Chiangc, Marc. However, recently, reducing the total power has become a main target, as IT power consumption has been

  11. Certification Program SIX SIGMA GREEN BELT

    E-Print Network [OSTI]

    Bandyopadhyay, Antar

    Certification Program for SIX SIGMA GREEN BELT 20th to 24th January 2015 at Kolkata. Copies of certificates for proof of Domicile 5. A participant will be declared as certified Green Belt a Green Belt Certificate. 6. Any candidate failing to qualify the exam will be given another chance

  12. Green Computing input for better outcomes

    E-Print Network [OSTI]

    Zhao, Feng

    Journal Profile: Udi Dahan Green Maturity Model for Virtualization Profiling Energy Usage for Efficient suggests that tracking energy consumption at every level will become the factor of success for greenGreen Computing input for better outcomes Learn the discipline, pursue the art, and contribute

  13. 24 January 2014 Green Impact Environmental Statement

    E-Print Network [OSTI]

    Kent, University of

    24 January 2014 Green Impact Environmental Statement Human Resources Human Resources is one of the University of Kent's professional services departments involved in Green Impact. We gained Bronze Awards with an Excellence Project which will engage other Registry colleagues. Green Impact offers schools and departments

  14. Promoting a Green Economy through Clean Transportation Alternatives...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives EV Community...

  15. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

    SciTech Connect (OSTI)

    Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

    2013-04-01

    Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ? Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ? Tri-molecular complexes containing two scFvs and one molecule GK were formed. ? GK was detected using fluorescence lifetime-based FRET imaging. ? GK is monomeric and homogeneously distributed in several cancer cell lines. ? This technique may have many applications in live-cell imaging of endogenous proteins.

  16. High-efficiency white organic light-emitting diodes using thermally activated delayed fluorescence

    SciTech Connect (OSTI)

    Nishide, Jun-ichi; Hiraga, Yasuhide; Nakanotani, Hajime; Adachi, Chihaya

    2014-06-09

    White organic light-emitting diodes (WOLEDs) have attracted much attention recently, aimed for next-generation lighting sources because of their high potential to realize high electroluminescence efficiency, flexibility, and low-cost manufacture. Here, we demonstrate high-efficiency WOLED using red, green, and blue thermally activated delayed fluorescence materials as emissive dopants to generate white electroluminescence. The WOLED has a maximum external quantum efficiency of over 17% with Commission Internationale de l'Eclairage coordinates of (0.30, 0.38).

  17. Fiber optical assembly for fluorescence spectrometry

    DOE Patents [OSTI]

    Carpenter, II, Robert W. (Pagosa Springs, CO); Rubenstein, Richard (Staten Island, NY); Piltch, Martin (Los Alamos, NM); Gray, Perry (Los Alamos, NM)

    2010-12-07

    A system for analyzing a sample for the presence of an analyte in a sample. The system includes a sample holder for containing the sample; an excitation source, such as a laser, and at least one linear array radially disposed about the sample holder. Radiation from the excitation source is directed to the sample, and the radiation induces fluorescent light in the sample. Each linear array includes a plurality of fused silica optical fibers that receive the fluorescent light and transmits a fluorescent light signal from the first end to an optical end port of the linear array. An end port assembly having a photo-detector is optically coupled to the optical end port. The photo-detector detects the fluorescent light signal and converts the fluorescent light signal into an electrical signal.

  18. Applications of Nuclear Resonance Fluorescence

    SciTech Connect (OSTI)

    Warren, Glen A.; Detwiler, Rebecca S.; Peplowski, Patrick N.

    2010-11-11

    Nuclear Resonance Fluorescence (NRF) has the potential of addressing a wide variety of applications, which require isotopic and/or elemental information about a sample. We have investigated a variety of non-proliferation applications that may be addressed by NRF. From these applications, we have selected two, measuring uranium enrichment in UF6 cylinders and material verification in dismantlement, to investigate in more detail. Analytical models have been developed to evaluate these applications, and test measurements have been conducted to validate those models. We found that it is unlikely with current technology to address the requirements for UF6 cylinder enrichment measurements. In contrast, NRF is a very promising approach for material verification for dismantlement.

  19. Home Articles Directions Contact Green Resources & Links The Green Building Gallery Posted by Branden Klayko: Broken Sidewalk.

    E-Print Network [OSTI]

    Home Articles Directions Contact Green Resources & Links The Green Building Gallery Posted, one door, 72 light fixtu

  20. From: Methods in Molecular Biology, vol. 350: Protein Folding Protocols Edited by: Y. Bai and R. Nussinov Humana Press Inc., Totowa, NJ

    E-Print Network [OSTI]

    Schuler, Ben

    115 From: Methods in Molecular Biology, vol. 350: Protein Folding Protocols Edited by: Y. Bai and R to Protein Folding Benjamin Schuler Summary Protein folding is a process characterized by a large degree this method to protein folding. Key Words: Protein folding; fluorescence spectroscopy; single molecule

  1. On the advantages of using green light to study fluorescence yield changes in leaves

    E-Print Network [OSTI]

    , at variance with the commonly used set-ups, uses of a weakly absorbed light source (light-emitting diodes. Although the exciting light is weakly absorbed, the possibility to tune the intensity of the light emitting diodes allows one to reach photochemical rates ranging from 104 s- 1 to 0.25 s- 1 rendering

  2. Simultaneous cryo x-ray ptychographic and fluorescence microscopy of green

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home RoomPreservation ofAlbuquerque|Sensitive Species3 Outlook forSimulations Yield Clues toalgae |

  3. Using Nuclear Resonance Fluorescence for Nondestructive Isotopic Analysis

    E-Print Network [OSTI]

    Ludewigt, Bernhard A.

    2010-01-01

    7, (1959) pp. 54. [12] B.J. Quiter, ``Nuclear ResonanceFluorescence for Nuclear Materials Assay,'' University ofclandestine material with nuclear resonance fluorescence,"

  4. Green Leases | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuels Data CenterFinancial OpportunitiesDepartment of Energy SmallGoogleGreen

  5. Glucose sensing molecules having selected fluorescent properties

    DOE Patents [OSTI]

    Satcher, Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-27

    An analyte sensing fluorescent molecule that employs intramolecular electron transfer is designed to exhibit selected fluorescent properties in the presence of analytes such as saccharides. The selected fluorescent properties include excitation wavelength, emission wavelength, fluorescence lifetime, quantum yield, photostability, solubility, and temperature or pH sensitivity. The compound comprises an aryl or a substituted phenyl boronic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. The fluorophore and switch component are selected such that the value of the free energy for electron transfer is less than about 3.0 kcal mol.sup.-1. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  6. 1206 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim DOI: 10.1002/cphc.200400609 ChemPhysChem 2005, 6, 1206 1220 Single-Molecule Fluorescence Spectroscopy of

    E-Print Network [OSTI]

    Schuler, Ben

    , 6, 1206 ­ 1220 #12;Single-Molecule Fluorescence Spectroscopy of Protein Folding Benjamin Schuler prevented a comprehensive solution of the protein-folding problem. Nevertheless, our understand- ing of protein-folding mechanisms has increased dramatically, and single-molecule spectroscopy has started

  7. A Phylogenetic Assessment of the Eukaryotic Light-Harvesting Antenna Proteins, with Implications for Plastid Evolution

    E-Print Network [OSTI]

    Durnford, Dion G.

    for Plastid Evolution D.G. Durnford,1 J.A. Deane,2 S. Tan,3 G.I. McFadden,2 E. Gantt,3 B.R. Green4 1 into the chlorophyll a/b-binding proteins of the green algae, euglenoids, and higher plants and the chlorophyll a, providing further evidence that its plastid evolved from a green algal secondary endosymbiosis

  8. NANOFLUIDIC SINGLE MOLECULE DETECTION (SMD) FOR PROTEIN DETECTION AND INTERACTION DYNAMICS STUDY 

    E-Print Network [OSTI]

    Jing, Nan

    2010-07-14

    , and proteins are electro-kinetically driven through the fluidic channel with external voltage source. Commercial functionalized Quantum Dots (Qdots) are used as fluorescent labels due to its various advantages over conventional organic dyes for single molecule...

  9. Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly

    E-Print Network [OSTI]

    Chang, Dongsook

    Bioconjugates of the model red fluorescent protein mCherry and synthetic polymer blocks with different hydrogen bonding functionalities show that the chemistry of the polymer block has a large effect on both ordering ...

  10. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

  11. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, Edward S. (Ames, IA); Taylor, John A. (Nevada, IA)

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  12. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, Edward S. (Ames, IA); Taylor, John A. (Nevada, IA)

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  13. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

  14. Fluorescent Lamp Ballasts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12, 2015ExecutiveFluorescent Lamp Ballasts Fluorescent Lamp Ballasts The

  15. The role of public policy in emerging green power markets: an analysis of marketer preferences

    E-Print Network [OSTI]

    Wiser, Ryan

    1999-01-01

    13 Green Power Market Facilitation Efforts . . . . . . . .Development of Green Power Markets . . . . . . . . . . . . .to the Development of the Green Power Market: Survey

  16. Green tea polyphenol (-)-epigallocatechin-3-gallate restores Nrf2 activity and ameliorates crescentic glomerulonephritis

    E-Print Network [OSTI]

    2015-01-01

    keratinocytes by green tea Constituent (-)-epigallocatechin-pathways by green tea polyphenol (-)-epigallocatechin-3-RESEARCH ARTICLE Green Tea Polyphenol (?)-Epigallocatechin-

  17. Laser excited confocal microscope fluorescence scanner and method

    DOE Patents [OSTI]

    Mathies, R.A.; Peck, K.

    1992-02-25

    A fluorescent scanner is designed for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier. The scanner includes a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from the volume to provide a display of the separated sample. 8 figs.

  18. City of Dallas Green Building Case Study 

    E-Print Network [OSTI]

    Basora, Z.

    2012-01-01

    Inspection City of Dallas Public Works Department City of Dallas Green Building Case Study CATEE CONFERENCE October 10, 2012 Dallas Building Inspection Take Aways ? Green Building Strategy Implementation is a process ? Awareness ? Education... Dallas Case Study 3 Dallas Building Inspection Municipal Green Building Program ? Adopted on January 22, 2003 ? all municipal projects over 10,000 s.f. to be LEED Silver Certified ? The City of Dallas has 21 LEED certified buildings, 9 gold, 11...

  19. Green's Functions in Perturbative Quantum Gravity

    E-Print Network [OSTI]

    Sudhaker Upadhyay; Bhabani Prasad Mandal

    2015-04-03

    We show that the Green's functions in non-linear gauge in the theory of perturbative quantum gravity is expressed as a series in terms of those in linear gauges. This formulation is also holds for operator Green's functions. We further derive the explicit relation between the Green's functions in the theory of perturbative quantum gravity in a pair of arbitary gauges. This process involves some sort of modified FFBRST transformations which is derivable from infinitesimal field-dependent BRST transformations.

  20. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01

    efficiency investments in buildings are made, and by examining the larger diffusion of green and energy efficient buildings throughout the urbanurban variables and the overall socio-cultural and regulatory environment that may make green labeling or energy efficiency

  1. Laminar-Flow Fluid Mixer for Fast Fluorescence Kinetics Studies Suzette A. Pabit and Stephen J. Hagen

    E-Print Network [OSTI]

    Hagen, Stephen J.

    Laminar-Flow Fluid Mixer for Fast Fluorescence Kinetics Studies Suzette A. Pabit and Stephen J i.d.) at a speed 20 cm/s, under laminar flow conditions (Re 14). Construction from a fused silica studies of fast protein and nucleic acid interactions and folding. We have constructed a laminar coaxial

  2. green

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal GasAdministration Medal01 Sandia4)9 Federal RegisterStorm1 3446 YEAR/%2Afissile4/%2A

  3. National Green Building Standard Analysis

    SciTech Connect (OSTI)

    none,

    2012-07-01

    DOE's Building America Program is a research and development program to improve the energy performance of new and existing homes. The ultimate goal of the Building America Program is to achieve examples of cost-effective, energy efficient solutions for all U.S. climate zones. Periodic maintenance of an ANSI standard by review of the entire document and action to revise or reaffirm it on a schedule not to exceed five years is required by ANSI. In compliance, a consensus group has once again been formed and the National Green Building Standard is currently being reviewed to comply with the periodic maintenance requirement of an ANSI standard.

  4. Green Power Program Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:Financing Tool Fits the Bill Financing Tool Fits27,Network Newsletter Green Power NetworkPartnerR R

  5. Green Button | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergy A plug-inPPLforLDRD Report11, SolarMat 4"GloriaGreen Button initiative|Nancy

  6. Sandia Energy - A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid youOxygen GenerationTechnologies |Education STEMA Green Technology Home

  7. NREL: Sustainable NREL -Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines lightGeospatial ToolkitSMARTS - Simple ModelCafeteriaVideos ofGreen

  8. Green Circle | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreen Button in theCircle

  9. Green Dragon | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreen Button in

  10. Green Highland | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreenHighland Jump to:

  11. Green Fuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE:FinancingPetroleum12,Executive CompensationEnergyGetDepartment of EnergyCollarGreen Fuel

  12. GreenGov Presidential Award

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journalvivo Low-Dose Lowï‚— We want USDOE to vitrifyGreenGov Presidential

  13. Final Green Zia Award Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journal Article) | SciTechSubmittedEcologist Final Site-WideDraftFinalGreen

  14. Green Energy Property Tax Assessment (Tennessee) | Department...

    Broader source: Energy.gov (indexed) [DOE]

    the Treasury Tennessee offers a special ad valorem property tax assessment for certified green energy production facilities. Property that generates electricity from a certified...

  15. ITEP Course: Greening Tribal Operations and Facilities

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Institute for Tribal Environmental Professionals will be offering a new course, Greening Tribal Operations and Facilities in San Diego, California, December 9 -11, 2014, for employees of...

  16. NIST Green Button Presentation | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Green.Button.webinar.for.DOE.Apps.Energy.pptx More Documents & Publications Electricity Advisory Committee Meeting Presentations March 2012 - Tuesday, March 6, 2012 SG Network...

  17. David L. Greene | ornl.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    geography in 1973 from the University of Oregon. He earned a doctorate in geography and environmental engineering in 1978 from Johns Hopkins University. Dr. Greene's work applies...

  18. Better Enzymes for Biofuels and Green Chemistry

    E-Print Network [OSTI]

    Better Enzymes for Biofuels and Green Chemistry: Solving the Cofactor Imbalance Problem Imbalances for the production of biofuels or other valuable chemicals. Though several research groups have re

  19. Green Manufacturing Initiative Annual Report 2010

    E-Print Network [OSTI]

    de Doncker, Elise

    Green Manufacturing Initiative Annual Report 2010 Dr. John Patten Dr. David Meade May 3, 2011 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Energy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Herman Miller Energy Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

  20. High Performance Builder Spotlight: Green Coast Enterprises ...

    Energy Savers [EERE]

    - New Orleans, Louisiana Building America Whole-House Solutions for New Homes: Green Coast Enterprises, New Orleans, Louisiana Building America Best Practices Series...

  1. Biochar: A Solution to Oakland's Green Waste?

    E-Print Network [OSTI]

    Villar, Amanda

    2012-01-01

    as an alternative waste management solution. Biochar is asequestration and alternative green waste management. For5 years, Alameda County Waste Management’s (WM) residential

  2. Vehicular Thermoelectrics: A New Green Technology | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    with the NSF deer11fairbanks.pdf More Documents & Publications Thermoelectrics: The New Green Automotive Technology Automotive Thermoelectric Generators and HVAC Solid-State...

  3. Green Purchasing under DOE Architect Engineer Contracts

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This clause should be used in any architect-engineer type contract unless Leadership in Energy and Environmental Design (LEED) Green Building Certification is being pursued. In...

  4. Trends in Utility Green Pricing Programs (2005)

    Broader source: Energy.gov [DOE]

    This report presents year-end 2005 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs. It is important to note that this report covers only a portion of voluntary markets for renewable energy. It does not cover green power sold by independent marketers except for cases in which the marketers work in conjunction with utilities or default electricity suppliers.

  5. Sustainable Manufacturing – Greening Processes, Systems and Products

    E-Print Network [OSTI]

    Dornfeld, David

    2010-01-01

    International Chemnitz Manufacturing Colloquium Prof. R.mittels Sustainable Manufacturing - Greening Processes,Annals - Dornfeld, D. A and its Manufacturing University of

  6. Appropriate use of Green Manufacturing Frameworks

    E-Print Network [OSTI]

    Reich-Weiser, Corinne; Vijayaraghavan, Athulan; Dornfeld, David

    2010-01-01

    Wedges for Implementing Green Manufacturing,” Trans.North American Manufacturing Research Institute, vol. 35,A. (2008), “Metrics for Manufacturing Sustainability,” Proc.

  7. Spectroscopic Properties of the CP43 Core Antenna Protein of Photosystem II

    E-Print Network [OSTI]

    van Stokkum, Ivo

    .e., they are involved in the capturing of solar energy, the efficient transfer of the exci- tation energy-protein complex that funnels excitation energy from the main light-harvesting system of photosystem II complex embedded in the thylakoid membranes of green plants, algae, and cyanobacteria. The green plant

  8. Air fluorescence relevant for cosmic-ray detection--Summary of the 5th fluorescence workshop, El Escorial 2007

    E-Print Network [OSTI]

    Hörandel, Jörg R.

    Air fluorescence relevant for cosmic-ray detection--Summary of the 5th fluorescence workshop, El August 2008 Keywords: Fluorescence yield Air showers a b s t r a c t High-energy cosmic rays with energies exceeding 1017 eV are frequently observed by measurements of the fluorescence light induced by air

  9. Ultratrace analysis of transuranic actinides by laser-induced fluorescence

    DOE Patents [OSTI]

    Miller, S.M.

    1983-10-31

    Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

  10. 3VCU Goes Green3VCU Goes Green VCU Director of Sustainability

    E-Print Network [OSTI]

    Hammack, Richard

    3VCU Goes Green3VCU Goes Green VCU Director of Sustainability Jacek Ghosh M Arch LEED AP CRMJacek Ghosh, M.Arch, LEED AP, CRM VCUgoes greenPPP Designed By: Jessica Hayashida, VCU Campus Sustainability Intern #12;VCU Office of Sustainability Areas of FocusAreas of Focus P t $Ad i i t ti Community

  11. This article was downloaded by:[Bowling Green State University] [Bowling Green State University

    E-Print Network [OSTI]

    McGovern, Warren W.

    This article was downloaded by:[Bowling Green State University] [Bowling Green State University] On representation that the contents will be complete or accurate or up to date. The accuracy of any instructions. © Taylor and Francis 2007 #12;DownloadedBy:[BowlingGreenStateUniversity]At:00:0621June2007 Communications

  12. GREEN BUILDING TRENDS How Green Building Impacted the National Capital Region Between 2003 and 2009

    E-Print Network [OSTI]

    GREEN BUILDING TRENDS How Green Building Impacted the National Capital Region Between 2003 and 2009 ecofriend.org 2 OVERVIEW What is LEED®? The LEED green building certification program is rapidly emerging as the standard in sustainable building design, construction, and operations. Scale of LEED Development Through

  13. VT GreenVT Green Engineering ProgramEngineering Program Freshman Information Session

    E-Print Network [OSTI]

    Beex, A. A. "Louis"

    ): Provide basic foundational ENGR 3124 ­ Intro to Green Engineering ENGR 3134 ­ Environmental Life Cycle engineering disciplines Courses (2): Provide broad context to tie engineering to economic, socialVT GreenVT Green Engineering ProgramEngineering Program Freshman Information Session O t b 20 2009

  14. PROPERTIES OF THE TOMBUSVIRUS MOVEMENT PROTEIN AND RNAi SUPPRESSOR THAT INFLUENCE PATHOGENESIS 

    E-Print Network [OSTI]

    Hsieh, Yi-Cheng

    2010-01-16

    Tomato bushy stunt virus (TBSV) provides a good model system to investigate molecular virus-host interactions in plants. P22 and P19 proteins encoded by TBSV contribute to multiple invasion-associated functions. Green ...

  15. Solving the 'Green Gap' in LED Technology

    Broader source: Energy.gov [DOE]

    One long-standing high-priority research area for DOE is to increase the efficiency of deep green LEDs. Although most products today use phosphor conversion (PC) to produce white light from a blue LED, having a good green source could lead to color-mixed white sources that would avoid the losses associated with the PC approach.

  16. Leafy Green Preparation and Cooling Study

    E-Print Network [OSTI]

    Leafy Green Preparation and Cooling Study Dominique N. Bibbins #12;Background : Leafy Greens z. #12;Background: Cooling Study z This study observes how restaurants keep their food cooled Food-borne illnesses may result when foods are improperly cooled or stored. #12;My Contribution z

  17. The Energy Institute Live Green, Burn Clean

    E-Print Network [OSTI]

    Lee, Dongwon

    The Energy Institute Live Green, Burn Clean: Advancing Engines for Renewable Fuels Live Green, Burn Clean: Advancing Engines for Renewable Fuels André Boehman Professor of Fuel Science and Materials College of Earth and Mineral Sciences The Pennsylvania State University André Boehman Professor of Fuel

  18. Information Brief on Green Power Marketing

    E-Print Network [OSTI]

    Information Brief on Green Power Marketing Fourth Edition August 1999 · NREL/TP-620-26901 Blair-26901 Information Brief on Green Power Marketing Fourth Edition Blair Swezey and Lori Bird Prepared under Task No for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed

  19. Customer Aggregation: An Opportunity for Green Power?

    E-Print Network [OSTI]

    Customer Aggregation: An Opportunity for Green Power? February 2001 · NREL/TP-620-29408 Edward Holt. DE-AC36-99-GO10337 February 2001 · NREL/TP-620-29408 Customer Aggregation: An Opportunity for Green or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process

  20. SOLAR TODAY28 The Green Mountain Energysm

    E-Print Network [OSTI]

    of the public and the renewable energy industry have worked diligently in regula- tory and public policy arenas, generates pollution-free, renewable electricity. GreenMountainEnergyCompany BuyingGreenPower-- You of renewable energy technologies. by Blair Swezey and Lori Bird #12;January/February 2003 29 The electricity

  1. Dynamic Spectrum Management for Green DSL

    E-Print Network [OSTI]

    this `green DSL' approach, we quantify the power-rate trade-off for different practical settings with some1 Dynamic Spectrum Management for Green DSL Paschalis Tsiaflakisa, , Yung Yib , Mung Chiangc , Marc the total power has become a main target, as IT power consumption has been identified as a significant

  2. COMPUTER POWER CONSUMPTION BENCHMARKING FOR GREEN COMPUTING

    E-Print Network [OSTI]

    Way, Thomas

    COMPUTER POWER CONSUMPTION BENCHMARKING FOR GREEN COMPUTING A Thesis Presented to the Faculty Computing Sciences Full Title of Thesis Computer Power Consumption Benchmarking for Green Computing Dr would like to thank my friend and colleague Christopher Continanza for our past collaborations on power

  3. GREEN TAXES, WASTE MANAGEMENT AND POLITICAL ECONOMY

    E-Print Network [OSTI]

    Bateman, Ian J.

    GREEN TAXES, WASTE MANAGEMENT AND POLITICAL ECONOMY by R. Kerry Turner J. Powell A. Craighill CSERGE Working Paper WM 96-03 #12;GREEN TAXES, WASTE MANAGEMENT AND POLITICAL ECONOMY by R. Kerry Turner and advocated. The application of such instruments in the current political economy settings will however serve

  4. Sustainable Design and Green Building Toolkit

    E-Print Network [OSTI]

    Sustainable Design and Green Building Toolkit F O R L O C A L G O V E R N M E N T S EPA 904B10001 | June 2010 #12;Disclaimer The Sustainable Design and Green Building Toolkit for Local Governments presented at non-EPA Web sites, or guarantee the validity of the information provided. iSustainable Design

  5. for Students, Staff & Visitors Green Guide

    E-Print Network [OSTI]

    Harman, Neal.A.

    for Students, Staff & Visitors Green Guide our environment...Partneriaith Addysg Uwch De Orllewin gwasanaethau · Working together to enhance services #12;Welcome ...to the 2008/2009 Swansea University Green by recycling as much as possible and by only using the energy and water you really need. This guide is packed

  6. 7 Systematics of the green algae: conflict of classic

    E-Print Network [OSTI]

    123 7 Systematics of the green algae: conflict of classic and modern approaches Thomas Pröschold ....................................................................................................................................124 How are green algae classified ....................................................................................................................................144 Biodiversity of green algae based on taxonomic revision using polyphasic approaches

  7. GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS, II

    E-Print Network [OSTI]

    Shoj, Toshiaki

    be described completely in terms of various "Gre* *en functions" of type Bn0. However, note that the Green GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS, II University of Tokyo Noda, Chiba 278-8510, Japan Abstract. Green functions

  8. The Economic Development Potential of the Green Sector

    E-Print Network [OSTI]

    Ong, Paul M.; Patraporn, Rita Varisa

    2006-01-01

    Green Power 2004 Annual Report,” Los Angeles Department ofGreen Technology Sector 2006”, Prepared by the Economic Roundtable, Los Angeles Los Angeles Department of Water and Power, “Green Business” include alternative fuel vehicles, biomass/waste-to energy power,

  9. A Green Prison: The Santa Rita Jail Campus Microgrid

    E-Print Network [OSTI]

    Marnay, Chris

    2013-01-01

    L ABORATORY A Green Prison: The Santa Rita Jail Campusopportunity employer. A Green Prison: The Santa Rita JailOften described as a green prison, it has a considerable

  10. Green Power Marketing in the United States: A Status Report

    E-Print Network [OSTI]

    ............................................................................................32 Retail Green Power MarketersGreen Power Marketing in the United States: A Status Report Sixth Edition October 2003 · NREL-35119 Green Power Marketing in the United States: A Status Report Sixth Edition Lori Bird and Blair Swezey

  11. Suggested Courses for ME Students Interested in Green Engineering Please see http://www.eng.vt.edu/green/ for information on the Green Engineering minor.

    E-Print Network [OSTI]

    Virginia Tech

    for increasing efficiencies (energy storage, batteries, green building, conservation). Options for transportationSuggested Courses for ME Students Interested in Green Engineering Please see http://www.eng.vt.edu/green/ for information on the Green Engineering minor. Required Courses: ME 4015-4016 ­ Engineering Design and Project (6

  12. GREEN GALAXIES IN THE COSMOS FIELD

    SciTech Connect (OSTI)

    Pan, Zhizheng; Kong, Xu; Fan, Lulu E-mail: xkong@ustc.edu.cn

    2013-10-10

    We present research on the morphologies, spectra, and environments of ?2350 'green valley' galaxies at 0.2 < z < 1.0 in the COSMOS field. The bimodality of dust-corrected NUV–r {sup +} color is used to define 'green valley'; it removes dusty star-forming galaxies from galaxies that are truly transitioning between the blue cloud and the red sequence. Morphological parameters of green galaxies are intermediate between those of blue and red galaxy populations, both on the Gini-asymmetry and the Gini-M{sub 20} planes. Approximately 60%-70% of green disk galaxies have intermediate or big bulges, and only 5%-10% are pure disk systems, based on morphological classification using the Zurich Estimator of Structural Types. The obtained average spectra of green galaxies are intermediate between blue and red ones in terms of [O II], H?, and H? emission lines. Stellar population synthesis on the average spectra shows that green galaxies are on average older than blue galaxies but younger than red galaxies. Green galaxies and blue galaxies have similar projected galaxy density (?{sub 10}) distributions at z > 0.7. At z < 0.7, the fractions of M{sub *} < 10{sup 10.0} M{sub ?} green galaxies located in a dense environment are found to be significantly larger than those of blue galaxies. The morphological and spectral properties of green galaxies are consistent with the transitioning population between the blue cloud and the red sequence. The possible mechanisms for quenching star formation activities in green galaxies are discussed. The importance of active galactic nucleus feedback cannot be well constrained in our study. Finally, our findings suggest that environmental conditions, most likely starvation and harassment, significantly affect the transformation of M{sub *} < 10{sup 10.0} M{sub ?} blue galaxies into red galaxies, especially at z < 0.5.

  13. From Tragedy to Triumph - Rebuilding Green Buildings after Disaster...

    Office of Environmental Management (EM)

    Rebuilding Green Buildings after Disaster, EERE (Fact Sheet) From Tragedy to Triumph - Rebuilding Green Buildings after Disaster, EERE (Fact Sheet) Fact sheet offering practical...

  14. Obama Administration Announces $12 Million i6 Green Investment...

    Broader source: Energy.gov (indexed) [DOE]

    The Obama Administration today announced the six winners of the i6 Green Challenge, an initiative to drive technology commercialization and entrepreneurship in support of a green...

  15. Attachment 3: Instructions for Use of Green Lease Solicitation...

    Energy Savers [EERE]

    Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart...

  16. Attachment 2: Solicitation for Offers with New and Revised Green...

    Energy Savers [EERE]

    Attachment 2: Solicitation for Offers with New and Revised Green Lease Text Attachment 2: Solicitation for Offers with New and Revised Green Lease Text PDF icon Solicitation for...

  17. Green Lease Policies and Procedures for Lease Acquisition | Department...

    Energy Savers [EERE]

    Green Lease Policies and Procedures for Lease Acquisition Green Lease Policies and Procedures for Lease Acquisition PDF icon RSL-2007-12 More Documents & Publications Attachment 1:...

  18. Highly Energy Efficient Directed Green Liquor Utilization (D...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Highly Energy Efficient Directed Green Liquor Utilization (D-GLU) Pulping Highly Energy Efficient Directed Green Liquor Utilization (D-GLU) Pulping This factsheet describes a...

  19. Obama Administration Announces $12 Million i6 Green Investment...

    Energy Savers [EERE]

    Obama Administration Announces 12 Million i6 Green Investment to Promote Clean Energy Innovation and Job Creation Obama Administration Announces 12 Million i6 Green Investment to...

  20. Obama Adminstration Announces Launch of i6 Green Challenge to...

    Energy Savers [EERE]

    Adminstration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth Obama Adminstration Announces Launch of i6 Green Challenge to Promote...

  1. Green Energy Resources Inc formerly New York International Log...

    Open Energy Info (EERE)

    Green Energy Resources Inc formerly New York International Log Lumber Company Jump to: navigation, search Name: Green Energy Resources Inc (formerly New York International Log &...

  2. Guide to Purchasing Green Power: Renewable Electricity, Renewable...

    Office of Environmental Management (EM)

    Purchasing Green Power: Renewable Electricity, Renewable Energy Certificates, and On-Site Renewable Generation Guide to Purchasing Green Power: Renewable Electricity, Renewable...

  3. Attachment 3: Instructions for Use of Green Lease Solicitation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart PDF icon...

  4. Obama Administration Announces Launch of i6 Green Challenge to...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Obama Administration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth Obama Administration Announces Launch of i6 Green Challenge to...

  5. Attachment 2: Solicitation for Offers with New and Revised Green...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2: Solicitation for Offers with New and Revised Green Lease Text Attachment 2: Solicitation for Offers with New and Revised Green Lease Text PDF icon Solicitation for Offers...

  6. Expanded "Green Button" Will Reach Federal Agencies and More...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    "Green Button" Will Reach Federal Agencies and More American Energy Consumers Expanded "Green Button" Will Reach Federal Agencies and More American Energy Consumers December 6,...

  7. City of Pittsburgh Implementation Model: Green Initiatives Trust...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Model: Green Initiatives Trust Fund City of Pittsburgh implementation model, Green initiatives trust fund. Author: U. S. Department of Energy City of Pittsburgh...

  8. Los Angeles County's Green Idea House Achieves Efficient Goals...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    County's Green Idea House Achieves Efficient Goals Los Angeles County's Green Idea House Achieves Efficient Goals Photo of an energy-efficient home with modern architecture. The...

  9. Forest County Potawatomi Tribe Cuts Emissions, Promotes Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Emissions, Promotes Green Growth Forest County Potawatomi Tribe Cuts Emissions, Promotes Green Growth February 23, 2012 - 6:29pm Addthis The Forest County Potawatomi Tribe's solar...

  10. Vice President Biden Unveils Report Focused on Expanding Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Unveils Report Focused on Expanding Green Jobs And Energy Savings For Middle Class Families Vice President Biden Unveils Report Focused on Expanding Green Jobs And Energy Savings...

  11. Low-Cost Electrochemical Compressor Utilizing Green Refrigerants...

    Office of Environmental Management (EM)

    Low-Cost Electrochemical Compressor Utilizing Green Refrigerants for HVAC Applications Low-Cost Electrochemical Compressor Utilizing Green Refrigerants for HVAC Applications...

  12. SEP Success Story: Launching Green Entrepreneurship in New Hampshire...

    Office of Environmental Management (EM)

    NH. | Courtesy of University of New Hampshire Photographic Services SEP Success Story: "Green Launching Pad" Supports Clean Energy Small Businesses SEP Success Story: Green...

  13. Webtrends Archives by Fiscal Year - Green Power and Greensburg...

    Energy Savers [EERE]

    Webtrends Archives by Fiscal Year - Green Power and Greensburg Webtrends Archives by Fiscal Year - Green Power and Greensburg From the EERE Web Statistics Archive: Corporate sites,...

  14. FEMP and Department of Transportation Partnership Wins GreenGov...

    Office of Environmental Management (EM)

    Steve Renzi, and Eugene Tumblin. Late last month, staff members from the Federal Energy Management Program (FEMP) were recognized with a GreenGov Presidential Green Team...

  15. The Energy Efficiency and Renewable Energy (EERE) blog on Green...

    Open Energy Info (EERE)

    The Energy Efficiency and Renewable Energy (EERE) blog on Green Button is live Home > Groups > Green Button Applications Dc's picture Submitted by Dc(266) Contributor 27 February,...

  16. Greening Federal Facilities: An Energy, Environmental, and Economic...

    Energy Savers [EERE]

    Greening Federal Facilities: An Energy, Environmental, and Economic Resource Guide for Federal Facility managers and Designers; Second Edition Greening Federal Facilities: An...

  17. Green Investment Horizons: Effects of Policy on the Market for...

    Open Energy Info (EERE)

    Green Investment Horizons: Effects of Policy on the Market for Building Energy Efficiency Technologies Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Green Investment...

  18. Korea's Green Growth Strategy: Mitigating Climate Change and...

    Open Energy Info (EERE)

    Korea's Green Growth Strategy: Mitigating Climate Change and Developing New Growth Engines Jump to: navigation, search Name Korea's Green Growth Strategy: Mitigating Climate Change...

  19. Expanded "Green Button" Will Reach Federal Agencies and More...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Expanded "Green Button" Will Reach Federal Agencies and More American Energy Consumers Expanded "Green Button" Will Reach Federal Agencies and More American Energy Consumers...

  20. Green Button: Enabling Energy Innovation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Button: Enabling Energy Innovation Green Button: Enabling Energy Innovation May 9, 2013 - 2:35pm Addthis Monisha Shah White House Council on Environmental Quality Nick Sinai...

  1. Green Button Energy Data Access Expanding Across America | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Button Energy Data Access Expanding Across America Green Button Energy Data Access Expanding Across America October 17, 2012 - 6:01pm Addthis Utilities demonstrating the...

  2. EECBG Success Story: David Crockett, Chattanooga's Green Frontiersman...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    David Crockett, Chattanooga's Green Frontiersman EECBG Success Story: David Crockett, Chattanooga's Green Frontiersman June 11, 2010 - 5:15pm Addthis Crockett has seen Chattanooga...

  3. Promoting a Green Economy through Clean Transportation Alternatives...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives EA-1816: Final...

  4. Ultrabright fluorescent OLEDS using triplet sinks

    DOE Patents [OSTI]

    Zhang, Yifan; Forrest, Stephen R; Thompson, Mark

    2013-06-04

    A first device is provided. The first device further comprises an organic light emitting device. The organic light emitting device further comprises an anode, a cathode, and an emissive layer disposed between the anode and the cathode. The emissive layer further comprises an organic host compound, an organic emitting compound capable of fluorescent emission at room temperature, and an organic dopant compound. The triplet energy of the dopant compound is lower than the triplet energy of the host compound. The dopant compound does not strongly absorb the fluorescent emission of the emitting compound.

  5. Thermalization of Green functions and quasinormal modes

    E-Print Network [OSTI]

    Justin R. David; Surbhi Khetrapal

    2015-05-12

    We develop a new method to study the thermalization of time dependent retarded Green function in conformal field theories holographically dual to thin shell AdS Vaidya space times. The method relies on using the information of all time derivatives of the Green function at the shell and then evolving it for later times. The time derivatives of the Green function at the shell is given in terms of a recursion formula. Using this method we obtain analytic results for short time thermalization of the Green function. We show that the late time behaviour of the Green function is determined by the first quasinormal mode. We then implement the method numerically. As applications of this method we study the thermalization of the retarded time dependent Green function corresponding to a minimally coupled scalar in the AdS3 and AdS5 thin Vaidya shells. We see that as expected the late time behaviour is determined by the first quasinormal mode. We apply the method to study the late time behaviour of the shear vector mode in AdS5 Vaidya shell. At small momentum the corresponding time dependent Green function is expected to relax to equilibrium by the shear hydrodynamic mode. Using this we obtain the universal ratio of the shear viscosity to entropy density from a time dependent process.

  6. GreenOrder | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County, Georgia:Oregon:Corp JumpGreen2V Jump to:GreenFireGreenOrder

  7. GreenTech Construction | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County, Georgia:Oregon:Corp JumpGreen2V JumpGreenShiftGreenTech

  8. Green racing at Argonne | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journalvivo Low-Dose Lowï‚— We want USDOE to vitrify allfromGreenGreenGreen

  9. Mechanisms of protein stabilization at very low pH ISBN 978-7-5487-0356-3

    E-Print Network [OSTI]

    Neshich, Goran

    Citrate-stabilized CdSe/CdS quantum dots as fluorescence probe for protein determination Effect IN STABILIZATION OF Cu-Zn-Al ALLOY Influence of pH value on chromate-free conversion coating for magnesium alloys

  10. Deep tissue fluorescence imaging and in vivo biological applications

    E-Print Network [OSTI]

    2012-01-01

    17(11) Crosignani et al. : Deep tissue fluorescence imaging16. D. Kobat et al. , “Deep tissue multiphoton microscopyV. Crosignani et al. , “ In vivo deep tissue fluorescence

  11. Imaging chromophores with undetectable fluorescence by stimulated emission microscopy

    E-Print Network [OSTI]

    Xie, Xiaoliang Sunney

    LETTERS Imaging chromophores with undetectable fluorescence by stimulated emission microscopy Wei, that is, spontaneous emission, is generally more sensitive than absorption measurement, and is widely used undetectable fluorescence because the spontaneous emission is dominated by theirfastnon-radiative decay3

  12. Chlorophyll Fluorescence Monitoring at Selected LTER

    E-Print Network [OSTI]

    Boyce, Richard L.

    no energy can go to photosynthesis · Thermal dissipation processes are usually minimal in the dark · So most of plant stress on photosynthesis in the field Fate of Light absorbed by Chlorophyll · Energy channeled to reaction center of PSII, used for photosynthesis ­ P · Thermal dissipation ­ D · Fluorescent emission ­ F

  13. Atomic and Molecular Fluorescence Excited by Photodissociation

    E-Print Network [OSTI]

    electron impact processes are compared with photodissociation. A bibliography of photo dissociation the strength a n d shape of fluorescence lines that arise from excited fragments produced in molecular photo more critical. T h e D o p p l e r width of the emission is important, however, since a larger width

  14. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

    1992-05-12

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

  15. Imaging properties of supercritical angle fluorescence optics

    E-Print Network [OSTI]

    Enderlein, Jörg

    Imaging properties of supercritical angle fluorescence optics J¨org Enderlein,1,4, Ingo Gregor,1.ruckstuhl@pci.uzh.ch 4http://www.joerg-enderlein.de enderlein@physik3.gwdg.de Abstract: In recent years, new optical the detection volume within one wavelength to an interface. For conventional optical systems with high numerical

  16. Bayesian fluorescence in situ hybridisation signal classification

    E-Print Network [OSTI]

    Lerner, Boaz

    hybridisation (FISH) signals for the detection of genetic abnormalities. Based on well-discriminating features hybridisation (FISH); Gaussian mixture model; Naive Bayesian classifier; Signal classification Artificial.artmed.2003.11.005 #12;1. Introduction Fluorescence in situ hybridisation (FISH) allows selective staining

  17. Oil Classification with Fluorescence Spectroscopy Engineering Physics

    E-Print Network [OSTI]

    Oldenburg, Carl von Ossietzky Universität

    detected by these channels. The investigation used three methods to examine crude oil, heavy oil, sludge1 Oil Classification with Fluorescence Spectroscopy Engineering Physics Master of Engineering and classification of oil spills on water surfaces. It is an overview of the laser remote sensor technique

  18. Purchasing Energy-Efficient Suspended Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    The Federal Energy Management Program (FEMP) provides acquisition guidance for suspended fluorescent luminaires, a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR-qualified or FEMP-designated products in all product categories covered by these programs and in any acquisition actions that are not specifically exempted by law.

  19. Apparatus for eliminating background interference in fluorescence measurements

    DOE Patents [OSTI]

    Martin, J.C.; Jett, J.H.

    1986-03-04

    The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.

  20. Text-Alternative Version: LED Replacements for Linear Fluorescent Lamps

    Broader source: Energy.gov [DOE]

    Below is the text-alternative version of the "LED Replacements for Linear Fluorescent Lamps" webcast, held June 20, 2011.

  1. Brownian dynamics without Green's functions

    SciTech Connect (OSTI)

    Delong, Steven; Donev, Aleksandar; Usabiaga, Florencio Balboa; Delgado-Buscalioni, Rafael; Griffith, Boyce E.; Leon H. Charney Division of Cardiology, Department of Medicine, New York University School of Medicine, New York, New York 10016

    2014-04-07

    We develop a Fluctuating Immersed Boundary (FIB) method for performing Brownian dynamics simulations of confined particle suspensions. Unlike traditional methods which employ analytical Green's functions for Stokes flow in the confined geometry, the FIB method uses a fluctuating finite-volume Stokes solver to generate the action of the response functions “on the fly.” Importantly, we demonstrate that both the deterministic terms necessary to capture the hydrodynamic interactions among the suspended particles, as well as the stochastic terms necessary to generate the hydrodynamically correlated Brownian motion, can be generated by solving the steady Stokes equations numerically only once per time step. This is accomplished by including a stochastic contribution to the stress tensor in the fluid equations consistent with fluctuating hydrodynamics. We develop novel temporal integrators that account for the multiplicative nature of the noise in the equations of Brownian dynamics and the strong dependence of the mobility on the configuration for confined systems. Notably, we propose a random finite difference approach to approximating the stochastic drift proportional to the divergence of the configuration-dependent mobility matrix. Through comparisons with analytical and existing computational results, we numerically demonstrate the ability of the FIB method to accurately capture both the static (equilibrium) and dynamic properties of interacting particles in flow.

  2. Green Destiny + mpiBLAST = Bioinfomatics

    E-Print Network [OSTI]

    Feng, Wu-chun

    The Components of "Bioinfomagic" · Green Destiny ­ A 240-node supercomputer in a "telephone booth" · Footprint: 6 filtration, no raised floor, and no humidifier/dehumidifier. · Computing Requirement ­ Parallel computer

  3. Microsoft Word - Green Building Certification EA

    Broader source: Energy.gov (indexed) [DOE]

    As required by Section 436(h) of EISA 2007, in 2008 GSA identified the U.S. Green Building Council's Leadership in Energy and Environmental Design (LEED) 5 Silver as...

  4. Greening the MLS for Business Intelligence

    Broader source: Energy.gov [DOE]

    "Greening the MLS for Business Intelligence," by Margaret O’Sullivan, RealEstate Business Intelligence, July 10, 2012. Presents an exploration of the MLS platform, key RETS standards, current trends, and future expectations.

  5. Energy Efficient Retrofits and Green Building Practices 

    E-Print Network [OSTI]

    Rahman, M.

    2010-01-01

    . Moreover, the increase in demand is also causing rise in pollution levels. Therefore, the subject of energy efficient retrofits and green building practices is becoming increasingly important. Based on the latest walkthrough energy audit it is proven...

  6. Green Button Momentum | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    their products. These companies include: Autodesk C3 EnerNOC Hara Johnson Controls Inc. Pulse Energy U.S. Green Data These companies join a growing universe of companies that have...

  7. Green Buildings, Environmental Awareness, and Organizational Image

    E-Print Network [OSTI]

    Rashid, Mahbub; Spreckelmeyer, Kent; Angrisano, Neal J.

    2012-01-01

    The paper reports a study that was conducted to understand the relationships between the occupants’ assessments of the environmental design features of a “green” building and their assessments of environmental awareness (EA) and organizational image...

  8. PSNC Energy (Gas)- Green Building Rate Discount

    Office of Energy Efficiency and Renewable Energy (EERE)

    This discounted rate is available to commercial customers whose building meets the U.S. Green Building Council’s Leadership in Energy and Environmental Design (LEED) certification or equivalent. To...

  9. Energy Innovation: Green Button Initiative Empowering Americans...

    Broader source: Energy.gov (indexed) [DOE]

    100 energy leaders and innovators recently gathered for a Green Button event in San Diego to share best practices and identify new applications, tools, and services that aim to...

  10. City of Dallas- Green Energy Purchasing

    Office of Energy Efficiency and Renewable Energy (EERE)

    Furthermore, the Dallas/Fort Worth International Airport is the highest-ranked airport for renewable energy use, separately ranking 7th on the Green Power Partner listing of local government gree...

  11. City of Santa Monica- Green Power Purchasing

    Broader source: Energy.gov [DOE]

    The City of Santa Monica made history June 1, 1999, as green electricity began powering all municipal facilities -- including the Santa Monica Airport, City Hall and the Santa Monica Pier -- making...

  12. South Carolina Municipalities- Green Power Purchasing

    Broader source: Energy.gov [DOE]

    Participating residential customers are able to purchase this green power for $3 per 100 kWh block. Commercial participants are able to purchase the power for $6 per 200 kWh block.

  13. Trends in Utility Green Pricing Programs (2006)

    SciTech Connect (OSTI)

    Bird, L.; Kaiser, M.

    2007-10-01

    In the early 1990s, only a handful of utilities offered their customers a choice of purchasing electricity generated from renewable energy sources. Today, more than 750 utilities--or about 25% of all utilities nationally--provide their customers a "green power" option. Through these programs, more than 70 million customers have the ability to purchase renewable energy to meet some portion or all of their electricity needs--or make contributions to support the development of renewable energy resources. Typically, customers pay a premium above standard electricity rates for this service. This report presents year-end 2006 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs.

  14. Trends in Utility Green Pricing Programs (2006)

    SciTech Connect (OSTI)

    Bird, Lori; Kaiser, Marshall

    2007-10-01

    In the early 1990s, only a handful of utilities offered their customers a choice of purchasing electricity generated from renewable energy sources. Today, more than 750 utilities—or about 25% of all utilities nationally—provide their customers a “green power” option. Through these programs, more than 70 million customers have the ability to purchase renewable energy to meet some portion or all of their electricity needs—or make contributions to support the development of renewable energy resources. Typically, customers pay a premium above standard electricity rates for this service. This report presents year-end 2006 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs.

  15. The economics of investing in green buildings

    E-Print Network [OSTI]

    Rizk, Charbel Maroun

    2010-01-01

    This thesis discusses economics of green buildings. The need to reduce greenhouse gases emissions became clear. Buildings account for a large part of the greenhouse gases emissions, changing the atmosphere's composition. ...

  16. Challenges in implementing green workforce development training

    E-Print Network [OSTI]

    Yeung, Louise H. (Louise Ho-Yee)

    2013-01-01

    To meet the labor demands of green economic development, workforce development programs are increasingly training low-income workers for living wage jobs that contribute to environmental services or benefits. Yet, effectively ...

  17. Green Libraries Are More Than Just Buildings

    E-Print Network [OSTI]

    Aulisio, George J.

    2013-01-01

    Green Building Council (USGBC) certification that stands for Leadership in Energygreen literature and are brainstorming ways to reduce the high-energygreen upgrade could be as simple as replacing all of the library’s light bulbs with energy

  18. Commissioning in a LEED & GOBAS Evaluated Green Building 

    E-Print Network [OSTI]

    Zhang, Y.

    2006-01-01

    scheme ???????? ?????????????? ???????? Special interests Special interests ??energy audit and assessmentenergy audit and assessment Commissioning in an Evaluated Green BuildingCommissioning in an Evaluated Green Building 99 ???? Method....620.20.487.2811.5463.1 Commissioning in an Evaluated Green BuildingCommissioning in an Evaluated Green Building 1717 ?????????????????????? Enthalpy Wheel for Heat RecoveryEnthalpy Wheel for Heat Recovery ???????? ???????????????????? ???????? Well designed, imported from Sweden...

  19. Green Roof Effect on Arthropod Biodiversity By Caitlin Race

    E-Print Network [OSTI]

    Ginzel, Matthew

    Green Roof Effect on Arthropod Biodiversity By Caitlin Race A green roof is a roof. Green roof technology has become increasingly popular due to several benefits including, insulation, runoff protection, and aesthetic value. This project was designed to see if a green roof would benefit

  20. last updated October 5, 20121 UNBC GREEN GRANT PROPOSAL GUIDELINES

    E-Print Network [OSTI]

    Northern British Columbia, University of

    , if applicable: 1. Project Summary Page Download and complete the one-page "Green Grant Project Summarylast updated October 5, 20121 UNBC GREEN GRANT PROPOSAL GUIDELINES In order to advance environmental sustainability at UNBC, a Green Fund was established in April 2009 and a Green Fund Subcommittee

  1. GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS, II

    E-Print Network [OSTI]

    Shoj, Toshiaki

    , that the Green functions of type D n can be described completely in terms of various ``Green functions'' of typeGREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS, II TOSHIAKI SHOJI Department of Mathematics Science University of Tokyo Noda, Chiba 278­8510, Japan Abstract. Green functions associated

  2. Green, Black, Lean Six Sigma and Master Black Belt Certifications

    E-Print Network [OSTI]

    Grissino-Mayer, Henri D.

    Green, Black, Lean Six Sigma and Master Black Belt Certifications Green BeLt CertifiCation Candidates may choose from three Green Belts offered: 1) Lean (one week of coursework) 2) Lean Six Sigma (two weeks of coursework) 3) Six Sigma (two weeks of coursework) Green Belt certification is a two

  3. ACADEMIA AND INDUSTRY FOR A GREEN (IT) SOCIETY

    E-Print Network [OSTI]

    Lefèvre, Laurent

    LINKING ACADEMIA AND INDUSTRY FOR A GREEN (IT) SOCIETY Christina Herzog, IRIT, University value e.g: Green hype, Green washing Freedom of Action Approach Search and find e.g: measuring load problems, "old wines in new bottles" High but limits through resources e.g: GreenIT allows breakthrough

  4. 10081 How snakes slither 10086 Biodegradable fluorescent polymers

    E-Print Network [OSTI]

    Yang, Jian

    10081 How snakes slither 10086 Biodegradable fluorescent polymers 10097 Studying forces within, and Michael J. Shelley (see pages 10081­10085) CHEMISTRY Biodegradable fluorescent polymers Although fluorescent polymers that can be crosslinked into membranes. The polymers may serve as scaffolds in tissue

  5. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01

    The Diffusion of Energy Efficiency in Building. ” Americanmake green or energy efficiency investments in buildings arefeatures or energy efficiency into commercial buildings, and

  6. Greening the Grid: The Role of Storage and Demand Response, Greening...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    STORAGE AND DEMAND RESPONSE GREENING THE GRID THE NEED FOR FLEXIBILITY Affordably integrating high levels of variable renewable energy (VRE) sources such as wind and solar requires...

  7. Protein Assay Technical Handbook

    E-Print Network [OSTI]

    Lebendiker, Mario

    Protein Assay Technical Handbook #12;Table of Contents Total Protein Assays Quick Technical......................................................................6 Selection of a Protein Standard....................................................................7 Standards for Total Protein Assay

  8. Jackson Park Hospital Green Building Medical Center

    SciTech Connect (OSTI)

    William Dorsey; Nelson Vasquez

    2010-03-01

    Jackson Park Hospital completed the construction of a new Medical Office Building on its campus this spring. The new building construction has adopted the City of Chicago�s recent focus on protecting the environment, and conserving energy and resources, with the introduction of green building codes. Located in a poor, inner city neighborhood on the South side of Chicago, Jackson Park Hospital has chosen green building strategies to help make the area a better place to live and work.

  9. GREEN PROGRAM This summer GREEN is launching a new program in Iceland, the "greenest" country in the

    E-Print Network [OSTI]

    Dawson, Jeff W.

    academic credit upon completion of the 10-day program. The GREEN Program enhances your understandingGREEN PROGRAM This summer GREEN is launching a new program in Iceland, the "greenest" country will receive the hands-on experience you need to accelerate your career. The GREEN Program is a short

  10. The Green Deal Value Chain: Stakeholders and business models for the delivery of the Green Deal and ECO

    E-Print Network [OSTI]

    Wrigley, Stuart

    &A panel with the energy suppliers to discuss the launch and delivery of Green Deal and ECO; explore howPower); Mark Cherry (Head of Green Deal ­ EON); Chris Ronketti (Head of Energy Solutions ­ British Gas BusinessThe Green Deal Value Chain: Stakeholders and business models for the delivery of the Green Deal

  11. Introduzione alle Proteine e al Protein Folding

    E-Print Network [OSTI]

    Giannozzi, Paolo

    Chapter 4 Introduzione alle Proteine e al Protein Folding 4.1 Proteine: propriet`a strutturali Le protein folding `e il cosiddetto modello HP, nel quale a ogni amino acido `e assegnata l'etichetta di

  12. The high risk HPV16 L2 minor capsid protein has multiple transport signals that mediate its nucleocytoplasmic traffic

    SciTech Connect (OSTI)

    Mamoor, Shahan; Onder, Zeynep [Biology Department, Boston College, Chestnut Hill, MA 02467 (United States)] [Biology Department, Boston College, Chestnut Hill, MA 02467 (United States); Karanam, Balasubramanyam; Kwak, Kihyuck [Department of Pathology, The Johns Hopkins University, Baltimore, MD 21231 (United States)] [Department of Pathology, The Johns Hopkins University, Baltimore, MD 21231 (United States); Bordeaux, Jennifer; Crosby, Lauren [Biology Department, Boston College, Chestnut Hill, MA 02467 (United States)] [Biology Department, Boston College, Chestnut Hill, MA 02467 (United States); Roden, Richard B.S. [Department of Pathology, The Johns Hopkins University, Baltimore, MD 21231 (United States)] [Department of Pathology, The Johns Hopkins University, Baltimore, MD 21231 (United States); Moroianu, Junona, E-mail: moroianu@bc.edu [Biology Department, Boston College, Chestnut Hill, MA 02467 (United States)] [Biology Department, Boston College, Chestnut Hill, MA 02467 (United States)

    2012-01-20

    In this study we examined the transport signals contributing to HPV16 L2 nucleocytoplasmic traffic using confocal microscopy analysis of enhanced green fluorescent protein-L2 (EGFP-L2) fusions expressed in HeLa cells. We confirmed that both nuclear localization signals (NLSs), the nNLS (1MRHKRSAKRTKR12) and cNLS (456RKRRKR461), previously characterized in vitro (Darshan et al., 2004), function independently in vivo. We discovered that a middle region rich in arginine residues (296SRRTGIRYSRIGNKQTLRTRS316) functions as a nuclear retention sequence (NRS), as mutagenesis of critical arginine residues within this NRS reduced the fraction of L2 in the nucleus despite the presence of both NLSs. Significantly, the infectivity of HPV16 pseudoviruses containing either RR297AA or RR297EE within the L2 NRS was strongly reduced both in HaCaT cells and in a murine challenge model. Experiments using Ratjadone A nuclear export inhibitor and mutation-localization analysis lead to the discovery of a leucine-rich nuclear export signal ({sub 462}LPYFFSDVSL) mediating 16L2 nuclear export. These data indicate that HPV16 L2 nucleocytoplasmic traffic is dependent on multiple functional transport signals.

  13. Composting 101 Prepared by the HLS Green Team and Green Living Program, updated February 2011

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Composting 101 Prepared by the HLS Green Team and Green Living Program, updated February 2011.5% was composted. The environmental effect of NOT trashing this mass would be equivalent to removing 5.8 million cars from the road. (www.epa.gov)1 What is Compost? Compost is the product of a biological process

  14. Composting at HLS Brought to you by the Green Team and Green Living Program February 2011

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Composting at HLS Brought to you by the Green Team and Green Living Program February 2011 Americans.5% was composted. The environmental effect of NOT trashing this mass would be equivalent to removing 5.8 million cars from the road. (www.epa.gov)1 Compost Basics Why Compost? 1 Emissions avoidance from compost

  15. GREEN BLUE CITY Visions of Green-Blue Infrastructure in the Salt Lake Valley

    E-Print Network [OSTI]

    Capecchi, Mario R.

    GREEN BLUE CITY Visions of Green-Blue Infrastructure in the Salt Lake Valley Memorial House, Memory will focus on the natural systems of land and water that support and enhance the economy, ecology and quality:55 SARAH HINNERS Ecological Planning Center 2:20 Break 2:35 JEFF NIERMEYER Salt Lake City Public Utilities

  16. GreenCoop: Cooperative Green Routing with Energy-efficient Servers

    E-Print Network [OSTI]

    GreenCoop: Cooperative Green Routing with Energy-efficient Servers Luca Chiaraviglio Dipartimento Energy-efficient communication has recently become a key challenge for both researchers and industries. In this paper, we propose a new model in which a Content Provider and an Internet Service Provider cooperate

  17. Index to the Digest of Green Reports and Studies Complete "Green"

    E-Print Network [OSTI]

    .org/green_technology_ma gazine/images/local_revolution.pdf www.labormarketinfo.edd.ca.gov/conte ntpub/GreenDigest/Benchmark- employment-in-Penn.pdf Berkeley Lab's Battery Team Looks Beyond Vehicles to the Electric Grid Julie Chao/07/29/battery-team- looks-beyond-vehicles-to-the-electric- grid/ www.labormarketinfo.edd.ca.gov/conte ntpub

  18. Analysis of the Vertebrate Insulator Protein CTCF-Binding

    E-Print Network [OSTI]

    D. Green,4 Michael Q. Zhang,3 Victor V. Lobanenkov,2 and Bing Ren1, * 1 Ludwig Institute for Cancer by preventing the spread of heterochromatin and restricting transcriptional enhancers from acti- vation ex- pressed nuclear protein with 11-zinc finger (ZF) DNA- binding domain (Filippova et al., 1996

  19. Invisible-fluorescent identification tags for materials

    SciTech Connect (OSTI)

    Lewis, Linda A.; Allgood, Glenn O.; Smithwick, III, Robert W.

    2013-03-26

    A taggant composition including a taggant material that is invisible in light of the visible spectrum and fluoresces under a non-visible excitation energy, a binder, and a solvent in which the taggant material and the binder are dissolved. The taggant composition can be printed or otherwise applied to a material such as fabric to provide a detectable and identifiable indicium. A method and apparatus for detecting and decoding the taggant indicium are also provided.

  20. Voluntary Green Power Market Forecast through 2015

    SciTech Connect (OSTI)

    Bird, L.; Holt, E.; Sumner, J.; Kreycik, C.

    2010-05-01

    Various factors influence the development of the voluntary 'green' power market--the market in which consumers purchase or produce power from non-polluting, renewable energy sources. These factors include climate policies, renewable portfolio standards (RPS), renewable energy prices, consumers' interest in purchasing green power, and utilities' interest in promoting existing programs and in offering new green options. This report presents estimates of voluntary market demand for green power through 2015 that were made using historical data and three scenarios: low-growth, high-growth, and negative-policy impacts. The resulting forecast projects the total voluntary demand for renewable energy in 2015 to range from 63 million MWh annually in the low case scenario to 157 million MWh annually in the high case scenario, representing an approximately 2.5-fold difference. The negative-policy impacts scenario reflects a market size of 24 million MWh. Several key uncertainties affect the results of this forecast, including uncertainties related to growth assumptions, the impacts that policy may have on the market, the price and competitiveness of renewable generation, and the level of interest that utilities have in offering and promoting green power products.

  1. Molecular dynamics and Monte Carlo simulations resolve apparent diffusion rate differences for proteins confined in nanochannels

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tringe, J. W.; Ileri, N.; Levie, H. W.; Stroeve, P.; Ustach, V.; Faller, R.; Renaud, P.

    2015-08-01

    We use Molecular Dynamics and Monte Carlo simulations to examine molecular transport phenomena in nanochannels, explaining four orders of magnitude difference in wheat germ agglutinin (WGA) protein diffusion rates observed by fluorescence correlation spectroscopy (FCS) and by direct imaging of fluorescently-labeled proteins. We first use the ESPResSo Molecular Dynamics code to estimate the surface transport distance for neutral and charged proteins. We then employ a Monte Carlo model to calculate the paths of protein molecules on surfaces and in the bulk liquid transport medium. Our results show that the transport characteristics depend strongly on the degree of molecular surface coverage.more »Atomic force microscope characterization of surfaces exposed to WGA proteins for 1000 s show large protein aggregates consistent with the predicted coverage. These calculations and experiments provide useful insight into the details of molecular motion in confined geometries.« less

  2. Fluorescence Rise Time Measurements for High Temperature Fluorescence-Based Thermometry

    SciTech Connect (OSTI)

    Allison, S.W.

    2005-03-24

    Certain ceramic-like phosphor materials exhibit bright fluorescence with a pronounced temperature dependence over a range which spans the cryogenic to 1700 C, depending on the specific phosphor. To measure temperature, a surface, for instance a turbine blade, is coated with the material. An optical system, sometimes including optical fibers, conveys stimulating light and collects the emission for analysis. Either emission intensity or decay time may indicate temperature. Previously fielded tests have involved surfaces such as blades, vanes, pistons, in-take valves, sheets of galvanneal steel, etc. The fluorescent coatings may be applied to small parts via sputtering methods or to large areas by mixture with inorganic binders. Presented here are results characterizing fluorescence rise times as a means of determining temperature from ambient to 700 C for Y{sub 2}O{sub 3}:Eu.

  3. Green tea polyphenol (-)-epigallocatechin-3-gallate restores Nrf2 activity and ameliorates crescentic glomerulonephritis

    E-Print Network [OSTI]

    2015-01-01

    keratinocytes by green tea Constituent (-)-epigallocatechin-multiple signaling pathways by green tea polyphenol (-)-therapy:is there a role for green tea? Kidney Int 2011; 80:

  4. Investigation of the Relationship between Green Design and Project Delivery Methods

    E-Print Network [OSTI]

    Bilec, Melissa M.

    2008-01-01

    the Relationship between Green Design and Project Deliveryis important on all green design projects independent of theparties involved in green design projects and it is a

  5. Hamerschlag Hall Green Roof Storm Water Retention and Runoff Reduction Performance Lucheng Chen

    E-Print Network [OSTI]

    Andrews, Peter B.

    Hamerschlag Hall Green Roof Storm Water Retention and Runoff Reduction Performance ......................................................................................................................... 2 2. Hamerschlag Hall Green Roof .............................................................................. 13 4. Methods of Storm Analysis and Green Roof Performance Analysis

  6. Public goods and private interests: Understanding non-residential demand for green power

    E-Print Network [OSTI]

    Wiser, Ryan H.; Fowlie, Meredith; Holt, Edward A.

    2001-01-01

    Understanding Non-Residential Demand for Green PowerUnderstanding Non-Residential Demand for Green Power Vining,Understanding Non-Residential Demand for Green Power Kasius,

  7. Public goods and private interests: Understanding non-residential demand for green power

    E-Print Network [OSTI]

    Wiser, Ryan H.; Fowlie, Meredith; Holt, Edward A.

    2001-01-01

    Residential Demand for Green Power References Andreoni, J.Approach to Marketing Green Power. ” Research Report No. 8.Residential Demand for Green Power Vining, J. , Linn, N. ,

  8. Green queue : a framework for selecting energy optimal DVFS congurations in large scale MPI applications

    E-Print Network [OSTI]

    Peraza, Joshua

    2012-01-01

    settings . . . . . Green Queue Energy Savings with VariousApplication Figure 4.3: Green Queue Energy Savings withBlind Scaling Relative Energy Green Queue Relative Delay

  9. High Performance Green LEDs by Homoepitaxial

    SciTech Connect (OSTI)

    Wetzel, Christian; Schubert, E Fred

    2009-11-22

    This work's objective was the development of processes to double or triple the light output power from green and deep green (525 - 555 nm) AlGaInN light emitting diode (LED) dies within 3 years in reference to the Lumileds Luxeon II. The project paid particular effort to all aspects of the internal generation efficiency of light. LEDs in this spectral region show the highest potential for significant performance boosts and enable the realization of phosphor-free white LEDs comprised by red-green-blue LED modules. Such modules will perform at and outperform the efficacy target projections for white-light LED systems in the Department of Energy's accelerated roadmap of the SSL initiative.

  10. Green's Function Approach to Inclusive Electron Scattering

    E-Print Network [OSTI]

    F. Capuzzi; C. Giusti; A. Meucci; F. D. Pacati

    2003-11-21

    A Green's function approach to the inclusive quasielastic ($e,e'$) scattering is presented. The components of the nuclear response are written in terms of the single-particle optical model Green's function. The explicit calculation of the Green's function can be avoided by its spectral representation, which is based on a biorthogonal expansion in terms of the eigenfunctions of the non-Hermitian optical potential and of its Hermitian conjugate. This allows one to treat final state interactions consistently in the inclusive ($e,e'$) and in the exclusive ($e,e'N$) reactions. Numerical results for the longitudinal and transverse response functions obtained in a nonrelativistic and in a relativistic framework are presented and discussed also in comparison with data.

  11. LANL/Green Star spectrometer tests

    SciTech Connect (OSTI)

    Sampson, T.E.; Cremers, T.L.; Vo, D.T. [Los Alamos National Lab., NM (United States); Seldiakov, Y.P.; Dorin, A.B.; Kondrashov, M.V. [Green Star, Moscow (Russian Federation); Timoshin, V.I. [VNIINM, Moscow (Russian Federation)

    1997-12-01

    The US and Russia have agreed to the joint development of a nondestructive assay system for use to support the dismantlement of nuclear weapons in Russia. This nondestructive assay system will be used to measure plutonium produced by the conversion of Russian nuclear weapons. The NDA system for Russia will be patterned after the ARIES NDA system being constructed at Los Alamos. One goal of the program is to produce an NDA system for use in Russia that maximizes the use of Russian resources to facilitate maintenance and future upgrades. The Green Star SBS50 Single Board Spectrometer system (Green Star Ltd., Moscow, Russia) has been suggested for use as the data acquisition component for gamma ray instruments in the system. Possible uses are for plutonium isotopic analysis and also segmented gamma scanning. Green Star has also developed analysis software for the SBS50. This software, both plutonium isotopic analysis and uranium enrichment analysis, was developed specifically for customs/border inspection applications (low counting rate applications and identification as opposed to quantification) and was not intended for MC and A applications. Because of the relative immaturity of the Green Star plutonium isotopic analysis software (it has been under development for only one year and is patterned after US development circa 1980), it was tentatively agreed, before the tests, that the Russian NDA system would use the Los Alamos PC/FRAM software for plutonium isotopic analysis. However, it was also decided to include the Green Star plutonium isotopic software in the testing, both to quantify its performance for MC and A applications and also to provide additional data to Green Star for further development of their software. The main purpose of the testing was to evaluate the SBS-50 spectrometer as a data acquisition device for use with LANL software.

  12. Development of anomalous diffusion among crowding proteins

    E-Print Network [OSTI]

    Margaret R. Horton; Felix Höfling; Joachim O. Rädler; Thomas Franosch

    2010-03-19

    In cell membranes, proteins and lipids diffuse in a highly crowded and heterogeneous landscape, where aggregates and dense domains of proteins or lipids obstruct the path of diffusing molecules. In general, hindered motion gives rise to anomalous transport, though the nature of the onset of this behavior is still under debate and difficult to investigate experimentally. Here, we present a systematic study where proteins bound to supported lipid membranes diffuse freely in two dimensions, but are increasingly hindered by the presence of other like proteins. In our model system, the surface coverage of the protein avidin on the lipid bilayer is well controlled by varying the concentration of biotinylated lipid anchors. Using fluorescence correlation spectroscopy (FCS), we measure the time correlation function over long times and convert it to the mean-square displacement of the diffusing proteins. Our approach allows for high precision data and a clear distinction between anomalous and normal diffusion. It enables us to investigate the onset of anomalous diffusion, which takes place when the area coverage of membrane proteins increases beyond approximately 5%. This transition region exhibits pronounced spatial heterogeneities. Increasing the packing fraction further, transport becomes more and more anomalous, manifested in a decrease of the exponent of subdiffusion.

  13. GreenShift Corporation | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County, Georgia:Oregon:Corp JumpGreen2V JumpGreenShift Corporation

  14. GreenTower | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History View New PagesSustainableGlynn County, Georgia:Oregon:Corp JumpGreen2VGreenTech Jump to:GreenTower

  15. Green Fuels Ltd | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreen ButtonGreen Fuels Ltd

  16. Green Gas International | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreen ButtonGreen

  17. Green Power Purchasing | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro Electric CoGreenHighlandLionGreen Power

  18. GreenTops | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EA Jump to:ofEniaElectricHydro ElectricGreen St.GreenTops Jump to:

  19. Green Button Applications | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages RecentPlantMagmaIncentives Jump to:energyGreen ButtonGreen

  20. Green Button FAQ | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages RecentPlantMagmaIncentives Jump to:energyGreenGreen Button

  1. Green Coast Enterprises | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages RecentPlantMagmaIncentives Jump to:energyGreenGreen

  2. Green Economy Toolbox | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages RecentPlantMagmaIncentives Jump to:energyGreenGreenEconomy

  3. GreenHomes America | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource History View New Pages RecentPlantMagmaIncentivesEnergy |GreenFever JumpGreenHomes

  4. Green Skies of Brazil |GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journalvivo Low-Dose Lowï‚— We want USDOE to vitrify allfromGreenGreen Skies

  5. Green Week 2011 | National Nuclear Security Administration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (Journalvivo Low-Dose Lowï‚— We want USDOE to vitrify allfromGreenGreen

  6. Home Articles Directions Contact Green Resources & Links The Green Building Gallery Gill Holland, green developer / film producer

    E-Print Network [OSTI]

    , green developer / film producer On March 13, 2006, my w ife Augusta and I bought a 110 year-old w environmental films and being acutely aw are of our being part of the problem in the increase of carbon in our

  7. In vivo multi-modality photoacoustic and pulse echo tracking of prostate tumor growth using a window chamber

    E-Print Network [OSTI]

    Witte, Russell S.

    with a dorsal skin flap window chamber. PC-3 prostate tumor cells, expressing green fluorescent protein (GFP. Keywords: optoacoustic, ultrasound, window chamber, prostate cancer, green fluorescent protein, GFP, cancer energy pulsed laser. As light penetrates the skin, its energy is partially absorbed and converted to heat

  8. Energy Savings and Green Initiatives Project Grant

    SciTech Connect (OSTI)

    Kathy MacLennan

    2011-11-21

    This project entails retrofitting all four foot, 2, 3 and 4 bulb 40 watt T12 fixtures to T8 28 watt and 150 watt incandescent to 26 watt compact fluorescent bulbs. In total, 2,086 fixtures will be retrofitted

  9. Solid state laser media driven by remote nuclear powered fluorescence

    DOE Patents [OSTI]

    Prelas, Mark A. (Columbia, MO)

    1992-01-01

    An apparatus is provided for driving a solid state laser by a nuclear powered fluorescence source which is located remote from the fluorescence source. A nuclear reaction produced in a reaction chamber generates fluorescence or photons. The photons are collected from the chamber into a waveguide, such as a fiber optic waveguide. The waveguide transports the photons to the remote laser for exciting the laser.

  10. England and Wales -A Competitive Electricity Richard Green

    E-Print Network [OSTI]

    California at Berkeley. University of

    PWP-060 England and Wales - A Competitive Electricity Market? Richard Green September 1998 Electricity Market? Richard Green * Department of Applied Economics, University of Cambridge Department, 1998 The British sometimes exaggerate their own importance. For example, we claim that the electricity

  11. WHEN THE BLUE-GREEN WATERS TURN RED

    E-Print Network [OSTI]

    WHEN THE BLUE-GREEN WATERS TURN RED Historical Flooding in Havasu Creek, Arizona U.S. GEOLOGICAL OF RECLAMATION #12;WHEN THE BLUE-GREEN WATERS TURN RED Historical Flooding in Havasu Creek, Arizona By THEODORE S

  12. Georgia: Data Center and Historic Municipal Building Go Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Georgia: Data Center and Historic Municipal Building Go Green Georgia: Data Center and Historic Municipal Building Go Green August 21, 2013 - 9:45am Addthis Data centers can...

  13. EA-1795: Diamond Green Diesel Facility in Norco, LA | Department...

    Office of Environmental Management (EM)

    5: Diamond Green Diesel Facility in Norco, LA EA-1795: Diamond Green Diesel Facility in Norco, LA April 1, 2011 EA-1795: Final Environmental Assessment Loan Guarantee to Diamond...

  14. Green Supers Graduate Prepared to Make NYC More Energy Efficient

    Office of Energy Efficiency and Renewable Energy (EERE)

    Secretary Chu delivered the graduation address for the Green Supers program in New York City. The 40-hour program teaches building superintendents everything they need to know about green building operations and maintenance.

  15. VEE-0076- In the Matter of Green Mountain Energy Company

    Broader source: Energy.gov [DOE]

    On August 23, 2000, the Green Mountain Energy Company (Green Mountain) of Austin, Texas, filed an Application for Exception with the Office of Hearings and Appeals of the Department of Energy (DOE)...

  16. The Greenness of Cities: Carbon Dioxide Emissions and Urban Development

    E-Print Network [OSTI]

    Glaeser, Edward L.; Kahn, Matthew E.

    2008-01-01

    Damage Costs of Carbon Dioxide Emissions: An Assessment ofThe Greenness of Cities: Carbon Dioxide Emissions and UrbanTHE GREENNESS OF CITIES: CARBON DIOXIDE EMISSIONS AND URBAN

  17. To Bridge LEDs' Green Gap, Scientists Think Small

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    To Bridge LEDs' Green Gap, Scientists Think Small To Bridge LEDs' Green Gap, Scientists Think Small Nanostructures Half a DNA Strand-Wide Show Promise for Efficient LEDs April 4,...

  18. Green Functions for the Radial Electric Component of the Monopole...

    Office of Scientific and Technical Information (OSTI)

    Green Functions for the Radial Electric Component of the Monopole Wake Field in a Round Resistive Chamber Citation Details In-Document Search Title: Green Functions for the Radial...

  19. Georgia: Data Center and Historic Municipal Building Go Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Municipal Building Go Green Georgia: Data Center and Historic Municipal Building Go Green August 21, 2013 - 9:45am Addthis Data centers can consume 100 to 200 times more...

  20. DOE Zero Energy Ready Home Case Study: Green Extreme Homes &...

    Energy Savers [EERE]

    Green Extreme Homes & Carl Franklin Homes, Garland, TX DOE Zero Energy Ready Home Case Study: Green Extreme Homes & Carl Franklin Homes, Garland, TX DOE Zero Energy Ready Home Case...