Sample records for green fluorescent protein

  1. GREEN FLUORESCENT PROTEIN The green revolution

    E-Print Network [OSTI]

    Stearns, Tim

    GREEN FLUORESCENT PROTEIN The green revolution Green fluorescent protein allows gene expression a fluorescent product when expressed. Just such a molecule, green fluorescent protein (GFP), has recently green light when disturbed (often seen when riding in a boat at night). In Aequorea, the green

  2. [16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions

    E-Print Network [OSTI]

    Raines, Ronald T.

    [16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions By SANG-HYUN PARK of reproduction in any form reserved. 0076-6879/00 $30.00 #12;252 A B OTHER APPROACHES USING CHIMERAS c ~S65T GFPHDII M 66 .......... 4S,.!;. 31 ui' 14 ..... [lB) FIG. 1. Green fluorescent protein chimera. (A

  3. Engineering and Characterization of a Superfolder Green Fluorescent Protein

    SciTech Connect (OSTI)

    Pedelacq,J.; Cabantous, S.; Tran, T.; Terwilliger, T.; Waldo, G.

    2006-01-01T23:59:59.000Z

    Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

  4. Split green fluorescent protein as a modular binding partner for protein crystallization

    SciTech Connect (OSTI)

    Nguyen, Hau B. [Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States); Hung, Li-Wei [Los Alamos National Laboratory, MS D454, Los Alamos, NM 87545 (United States); Yeates, Todd O. [University of California, PO Box 951569, Los Angeles, CA 90095 (United States); Terwilliger, Thomas C., E-mail: terwilliger@lanl.gov; Waldo, Geoffrey S., E-mail: terwilliger@lanl.gov [Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545 (United States)

    2013-12-01T23:59:59.000Z

    A strategy using a new split green fluorescent protein (GFP) as a modular binding partner to form stable protein complexes with a target protein is presented. The modular split GFP may open the way to rapidly creating crystallization variants. A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP ?-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10–11) hairpin in complex with GFP(1–9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10–11) hairpin with a variety of GFP(1–9) mutants engineered for favorable crystallization.

  5. Proton Pathways in Green Fluorescence Protein Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

    E-Print Network [OSTI]

    Agmon, Noam

    Proton Pathways in Green Fluorescence Protein Noam Agmon Department of Physical Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel ABSTRACT Proton pathways in green fluorescent protein pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton

  6. On the rough folding landscape of green fluorescent protein

    E-Print Network [OSTI]

    Andrews, Benjamin Thomas

    2008-01-01T23:59:59.000Z

    H. (2008). Understanding protein folding: small proteins inG. (1997). Theory of protein folding: the energy landscapeenergy landscape of protein folding: a synthesis. Proteins

  7. Fluorescent Protein Applications in Microscopy

    E-Print Network [OSTI]

    Straight, Aaron

    . The Identification of Green Fluorescent Protein III. Formation of the GFP Chromophore IV. The Structure of GFP V environment. II. The Identification of Green Fluorescent Protein The isolation of green fluorescent protein of Aequorea, Shimomura et al. noted that the lumines- cence from aequorin was blue rather than the green

  8. First-Principles Characterization of the Energy Landscape and Optical Spectra of Green Fluorescent Protein along the AIB

    E-Print Network [OSTI]

    Krylov, Anna I.

    First-Principles Characterization of the Energy Landscape and Optical Spectra of Green Fluorescent Supporting Information ABSTRACT: Structures and optical spectra of the green fluorescent protein (GFP) forms-form) is lowest in energy, whereas the systems with the anionic chromophore (B- and I-forms) are about 1 kcal

  9. aequorea green fluorescent: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    plants. Jim Haseloff Biology and Medicine Websites Summary: light, and an accessory green fluorescent protein (GFP), which accepts energy from aequorin and reImaging green...

  10. Computational Prediction of Absorbance Maxima for a Structurally Diverse Series of Engineered Green Fluorescent Protein Chromophores

    E-Print Network [OSTI]

    Campbell, Robert E.

    Fluorescent Protein Chromophores Qadir K. Timerghazin, Haley J. Carlson, Chen Liang, Robert E. Campbell,* and Alex Brown* UniVersity of Alberta, Department of Chemistry, Edmonton, Alberta T6G 2G2, Canada Recei

  11. Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/ H148D. 2. Unusual Photophysical Properties

    E-Print Network [OSTI]

    Boxer, Steven G.

    Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/ H148D. 2. Unusual, California 94305-5080, and Institute of Molecular Biology and Department of Physics, UniVersity of Oregon of this variant at pH 5.6 by ultrafast fluorescence upconversion spectroscopy. Following excitation at 400 nm

  12. Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/ H148D. 1. Mutagenesis and Structural Studies,

    E-Print Network [OSTI]

    Boxer, Steven G.

    Articles Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/ H148D. 1 Kanchanawong,# William Childs,# Steven G. Boxer,# and S. James Remington*,§ Institute of Molecular Biology chromophores, respectively. Excitation of either band leads to green emission. In wt-GFP, excitation of band

  13. Competition between photodetachment and autodetachment of the 2{sup 1}??{sup *} state of the green fluorescent protein chromophore anion

    SciTech Connect (OSTI)

    Mooney, Ciarán R. S.; Parkes, Michael A.; Zhang, Lijuan; Hailes, Helen C.; Fielding, Helen H., E-mail: h.h.fielding@ucl.ac.uk [Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ (United Kingdom); Simperler, Alexandra; Bearpark, Michael J. [Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ (United Kingdom)

    2014-05-28T23:59:59.000Z

    Using a combination of photoelectron spectroscopy measurements and quantum chemistry calculations, we have identified competing electron emission processes that contribute to the 350–315 nm photoelectron spectra of the deprotonated green fluorescent protein chromophore anion, p-hydroxybenzylidene-2,3-dimethylimidazolinone. As well as direct electron detachment from S{sub 0}, we observe resonant excitation of the 2{sup 1}??* state of the anion followed by autodetachment. The experimental photoelectron spectra are found to be significantly broader than photoelectron spectrum calculated using the Franck-Condon method and we attribute this to rapid (?10 fs) vibrational decoherence, or intramolecular vibrational energy redistribution, within the neutral radical.

  14. DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein Cliff I. Stains, Jason R. Porter, Aik T. Ooi, David J. Segal,*, and Indraneel Ghosh*,

    E-Print Network [OSTI]

    Ghosh, Indraneel

    DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein Cliff I. Stains, Jason R. Porter this principle of sequence-enabled activity in the rational design of a split protein system2 that reassembles only in the presence of a specific DNA sequence (Figure 1) to allow for direct detection of DNA

  15. DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein Cliff I. Stains, Jason Porter, Aik T. Ooi, David J. Segal and Indraneel Ghosh*

    E-Print Network [OSTI]

    Ghosh, Indraneel

    1 DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein Cliff I. Stains, Jason Porter ligase, dNTPS were obtained from New England Biolabs. Initial Cloning: NGFP and CGFP coding DNA sequences primers based upon published sequences)3 and PBSII (gift from the Barbas Laboratory)4 coding regions

  16. Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera

    SciTech Connect (OSTI)

    Hernaez, Bruno [Departamento de Biotecnologia, INIA, Carretera de la Coruna Km 7, 28040 Madrid (Spain)]. E-mail: hernaez@inia.es; Escribano, Jose M. [Departamento de Biotecnologia, INIA, Carretera de la Coruna Km 7, 28040 Madrid (Spain)]. E-mail: escriban@inia.es; Alonso, Covadonga [Departamento de Biotecnologia, INIA, Carretera de la Coruna Km 7, 28040 Madrid (Spain)]. E-mail: calonso@inia.es

    2006-06-20T23:59:59.000Z

    Many stages of African swine fever virus infection have not yet been studied in detail. To track the behavior of African swine fever virus (ASFV) in the infected cells in real time, we produced an infectious recombinant ASFV (B54GFP-2) that expresses and incorporates into the virus particle a chimera of the p54 envelope protein fused to the enhanced green fluorescent protein (EGFP). The incorporation of the fusion protein into the virus particle was confirmed immunologically and it was determined that p54-EGFP was fully functional by confirmation that the recombinant virus made normal-sized plaques and presented similar growth curves to the wild-type virus. The tagged virus was visualized as individual fluorescent particles during the first stages of infection and allowed to visualize the infection progression in living cells through the viral life cycle by confocal microscopy. In this work, diverse potential applications of B54GFP-2 to study different aspects of ASFV infection are shown. By using this recombinant virus it was possible to determine the trajectory and speed of intracellular virus movement. Additionally, we have been able to visualize for first time the ASFV factory formation dynamics and the cytophatic effect of the virus in live infected cells. Finally, we have analyzed virus progression along the infection cycle and infected cell death as time-lapse animations.

  17. Improved ``Optical Highlighter'' Probes Derived from Discosoma Red Fluorescent Protein

    E-Print Network [OSTI]

    Marchant, Jonathan

    , Minneapolis, Minnesota ABSTRACT The tetrameric red fluorescent protein, DsRed, undergoes a rapid red to green-emitting species of DsRed and an enhancement of emission from the ``immature'' green species, likely caused by dequenching of fluorescence resonance energy transfer occurring within the protein tetramer. Here, we have

  18. Teal fluorescent proteins: Characterization of a reversibly photoswitchable variant

    E-Print Network [OSTI]

    Campbell, Robert E.

    libraries of variants. mTFP1 has proven to be a particularly useful new member of the FP `toolbox green fluorescent protein (GFP) in 1961 when he was isolating aequorin, a calcium improved variants of GFP, including the variant known as enhanced green fluorescent protein (EGFP), were

  19. Recombinant fluorescent protein microsphere calibration standard

    DOE Patents [OSTI]

    Nolan, John P. (Santa Fe, NM); Nolan, Rhiannon L. (Santa Fe, NM); Ruscetti, Teresa (Los Alamos, NM); Lehnert, Bruce E. (Los Alamos, NM)

    2001-01-01T23:59:59.000Z

    A method for making recombinant fluorescent protein standard particles for calibration of fluorescence instruments.

  20. Modeling green fluorescent protein transcription, translation and modification as a method to obtain NF-kappaB activation profiles

    E-Print Network [OSTI]

    Laible, Allyson Marie

    2009-05-15T23:59:59.000Z

    that fluorescence increases up to 24 hours after an initial delay of approximately four hours. The fluorescence data was also used to develop a model describing significant events leading to NF-?B activation and GFP expression. In addition, a model describing...

  1. Use of green fluorescent fusion protein to track activation of the transcription factor osterix during early osteoblast differentiation

    SciTech Connect (OSTI)

    Tai Guangping [Tissue Engineering and Regenerative Medicine Centre, Imperial College London, Chelsea and Westminster Campus, London (United Kingdom); Christodoulou, Ioannis [Tissue Engineering and Regenerative Medicine Centre, Imperial College London, Chelsea and Westminster Campus, London (United Kingdom); Bishop, Anne E. [Tissue Engineering and Regenerative Medicine Centre, Imperial College London, Chelsea and Westminster Campus, London (United Kingdom); Polak, Julia M. [Tissue Engineering and Regenerative Medicine Centre, Imperial College London, Chelsea and Westminster Campus, London (United Kingdom)]. E-mail: julia.polak@imperial.ac.uk

    2005-08-12T23:59:59.000Z

    Osterix (Osx) is a transcription factor required for the differentiation of preosteoblasts into fully functioning osteoblasts. However, the pattern of Osx activation during preosteoblast differentiation and maturation has not been clearly defined. Our aim was to study Osx activation during these processes in osteoblasts differentiating from murine and human embryonic stem cells (ESC). To do this, we constructed an Osx-GFP fusion protein reporter system to track Osx translocation within the cells. The distribution of Osx-GFP at representative stages of differentiation was also investigated by screening primary osteoblasts, mesenchymal stem cells, synoviocytes, and pre-adipocytes. Our experiments revealed that Osx-GFP protein was detectable in the cytoplasm of cultured, differentiated ESC 4 days after plating of enzymatically dispersed embryoid bodies. Osterix-GFP protein became translocated into the nucleus on day 7 following transfer of differentiated ESC to osteogenic medium. After 14 days of differentiation, cells showing nuclear translocation of Osx-GFP formed rudimentary bone nodules that continued to increase in number over the following weeks (through day 21). We also found that Osx translocated into the nuclei of mesenchymal stem cells (C3H10T1/2) and pre-osteoblasts (MC3T3-E1) and showed partial activation in pre-adipocytes (MC3T3-L1). These data suggest that Osx activation occurs at a very early point in the differentiation of the mesenchymal-osteoblastic lineage.

  2. Engineering novel fluorescent proteins

    E-Print Network [OSTI]

    Shaner, Nathan Christopher

    2006-01-01T23:59:59.000Z

    pellet by QIAprep spin column (Qiagen) and submitted for sequencing. Protein Productionpellets by QIAprep spin column (Qiagen) and submitted for sequencing. Protein production

  3. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01T23:59:59.000Z

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  4. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2011-06-07T23:59:59.000Z

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  5. Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM)

    2007-09-18T23:59:59.000Z

    The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

  6. Protein- protein interaction detection system using fluorescent protein microdomains

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2010-02-23T23:59:59.000Z

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  7. Protein subcellular localization assays using split fluorescent proteins

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2009-09-08T23:59:59.000Z

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  8. Blue-green phosphor for fluorescent lighting applications

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15T23:59:59.000Z

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  9. LucY: A Versatile New Fluorescent Reporter Protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Auldridge, Michele E.; Cao, Hongnan; Sen, Saurabh; Franz, Laura P.; Bingman, Craig A.; Yennamalli, Ragothaman M.; Phillips, George N.; Mead, David; Steinmetz, Eric J.; Michnick, Stephen W.

    2015-04-23T23:59:59.000Z

    We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276nm, 377nm, and 460nm and a single emission peak at 530nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrastmore »to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.« less

  10. Subunits of highly Fluorescent Protein R-Phycoerythrin as Probes for Cell Imaging and Single-Molecule Detection

    SciTech Connect (OSTI)

    Dragan Isailovic

    2005-12-17T23:59:59.000Z

    The purposes of our research were: (1) To characterize subunits of highly fluorescent protein R-Phycoerythrin (R-PE) and check their suitability for single-molecule detection (SMD) and cell imaging, (2) To extend the use of R-PE subunits through design of similar proteins that will be used as probes for microscopy and spectral imaging in a single cell, and (3) To demonstrate a high-throughput spectral imaging method that will rival spectral flow cytometry in the analysis of individual cells. We first demonstrated that R-PE subunits have spectroscopic and structural characteristics that make them suitable for SMD. Subunits were isolated from R-PE by high-performance liquid chromatography (HPLC) and detected as single molecules by total internal reflection fluorescence microscopy (TIRFM). In addition, R-PE subunits and their enzymatic digests were characterized by several separation and detection methods including HPLC, capillary electrophoresis, sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE) and HPLC-electrospray ionization mass spectrometry (ESI-MS). Favorable absorption and fluorescence of the R-PE subunits and digest peptides originate from phycoerythrobilin (PEB) and phycourobilin (PUB) chromophores that are covalently attached to cysteine residues. High absorption coefficients and strong fluorescence (even under denaturing conditions), broad excitation and emission fluorescence spectra in the visible region of electromagnetic spectrum, and relatively low molecular weights make these molecules suitable for use as fluorescence labels of biomolecules and cells. We further designed fluorescent proteins both in vitro and in vivo (in Escherichia coli) based on the highly specific attachment of PEB chromophore to genetically expressed apo-subunits of R-PE. In one example, apo-alpha and apo-beta R-PE subunits were cloned from red algae Polisiphonia boldii (P. boldii), and expressed in E. coli. Although expressed apo-subunits formed inclusion bodies, fluorescent holo-subunits were formed after incubation of E. coli cells with PEB. Spectroscopic characterization of holo-subunits confirmed that the attachment of PEB chromophore to apo-subunits yielded holo-subunits containing both PEB and urobilin (UB). Fluorescence and differential interference contrast (DIC) microscopy showed polar location of holo-subunit inclusion bodies in E. coli cells. In another example, R-PE apo-subunits were genetically fused to cytoplasmic and periplasmic versions of E. coli maltose binding protein (MBP). Fluorescent proteins formed after attachment of PEB to MBP-subunit fusions in vitro and in vivo contained PEB as the sole chromophore, were soluble, and displayed high orange fluorescence. Fluorescence microscopy showed that fusions are located either throughout cells or at cell poles. In addition, cells containing fluorescent holo-subunits or MBP-subunit fusions were up to ten times brighter than control cells as measured by flow cytometry. Results show that the fluorescent proteins formed after non-enzymatic attachment of PEB to R-PE subunit fusions could be used as reporters of gene expression and protein localization in cells as well as fluorescence labels in flow cytometry. Finally, we demonstrated a high-throughput method able to record emission fluorescence spectra of individual cells containing fluorescent proteins. Upon excitation with a 488 mn argon-ion laser many bacterial cells were imaged by a 20X microscope objective while they moved through a capillary tube. Fluorescence was dispersed by a transmission diffraction grating, and an intensified charge-coupled device (ICCD) camera simultaneously recorded the zero and the first orders of the fluorescence from each cell. Single-cell fluorescence spectra were reconstructed from the distance between zero-order and first-order maxima as well as the length and the pixel intensity distribution of the first-order images. By using this approach, the emission spectrum of E. coli cells expressing green fluorescent protein (GFP) was reconstructed. Also, fluorescence spectra of E

  11. An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

    E-Print Network [OSTI]

    Verkhusha, Vladislav V.

    An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore Commonly used monomeric blue fluorescent proteins suffer from moderate brightness. The brightest of them, m transition from a blue fluorescent state with absorbance at 401 nm to a non-fluorescent state with absorbance

  12. Enhanced Archaerhodopsin Fluorescent Protein Voltage Yiyang Gong1,2*

    E-Print Network [OSTI]

    Ferrari, Silvia

    Enhanced Archaerhodopsin Fluorescent Protein Voltage Indicators Yiyang Gong1,2* , Jin Zhong Li1 by nearly three-fold in comparison to Arch-D95N. Citation: Gong Y, Li JZ, Schnitzer MJ (2013) Enhanced; Published June 19, 2013 Copyright: © 2013 Gong et al. This is an open-access article distributed under

  13. SciTech Connect: The Protein-Chlorophyll-770 Complex From Green...

    Office of Scientific and Technical Information (OSTI)

    Protein-Chlorophyll-770 Complex From Green Bacteria Citation Details In-Document Search Title: The Protein-Chlorophyll-770 Complex From Green Bacteria In the present paper, the...

  14. Rotational order–disorder structure of fluorescent protein FP480

    SciTech Connect (OSTI)

    Pletnev, Sergei, E-mail: svp@ncifcrf.gov [SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); Morozova, Kateryna S.; Verkhusha, Vladislav V. [Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461 (United States); Dauter, Zbigniew, E-mail: svp@ncifcrf.gov [Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States); SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439 (United States)

    2009-09-01T23:59:59.000Z

    An analysis of the rotational order–disorder structure of fluorescent protein FP480 is presented. In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

  15. Extending the utility of enzymes for site-specific targeting of fluorescent probes

    E-Print Network [OSTI]

    Liu, Daniel S. (Daniel Shao-Chen)

    2014-01-01T23:59:59.000Z

    Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, ...

  16. Cyanine-based probe\\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2013-01-15T23:59:59.000Z

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  17. Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein John T. M. Kennis,*,

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Ultrafast Proton Shuttling in Psammocora Cyan Fluorescent Protein John T. M. Kennis,*, Ivo H. M-state proton transfer (ESPT) reactions. Recently, a novel cyan fluorescent protein (CFP) termed psamFP488 vicinity to the chromophore to act as a proton acceptor. Our findings support a model where unusually fast

  18. Improving the photostability of bright monomeric orange and red fluorescent proteins

    E-Print Network [OSTI]

    Tsien, Roger Y.

    Improving the photostability of bright monomeric orange and red fluorescent proteins Nathan C illumination. Although fluorescent proteins typically bleach at a substantially slower rate than many small-molecule dyes, in many cases the lack of sufficient photostability remains an important limiting factor

  19. X-ray crystal structure and properties of Phanta, a weakly fluorescent photochromic GFP-like protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Paul, Craig Don; Traore, Daouda A. K.; Olsen, Seth; Devenish, Rodney J.; Close, Devin W.; Bell, Toby D. M.; Bradbury, Andrew; Wilce, Matthew C. J.; Prescott, Mark

    2015-04-29T23:59:59.000Z

    Phanta is a reversibly photoswitching chromoprotein (?F, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (?F, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in combination, to the optical properties of Phanta. Single amino acid substitutions at position 193 resulted in proteins with very low ?F, indicating the importance of this position in controlling the fluorescence efficiency of the variant proteins. The substitution Thr69Val in Phanta was important for supressing the formation of a protonated chromophore species observed in some His193 substituted variants,more »whereas the substitution Gln62Met did not significantly contribute to the useful optical properties of Phanta. X-ray crystal structures for Phanta (2.3 Å), eCGP123T69V (2.0 Å) and eCGP123H193Q (2.2 Å) in their non-photoswitched state were determined, revealing the presence of a cis-coplanar chromophore. We conclude that changes in the hydrogen-bonding network supporting the cis-chromophore, and its contacts with the surrounding protein matrix, are responsible for the low fluorescence emission of eCGP123 variants containing a His193 substitution.« less

  20. Screening Libraries To Identify Proteins with Desired Binding Activities Using a Split-GFP

    E-Print Network [OSTI]

    Shorter, James

    Screening Libraries To Identify Proteins with Desired Binding Activities Using a Split 06520 cbi9990825210008 W e have previously described a split-green fluorescent protein (GFP) reassembly assay by which to detect protein protein interac- tions (1-3). In this assay, green fluorescent protein

  1. In vitro analysis of ER-associated protein degradation

    E-Print Network [OSTI]

    Garza, Renee Marie

    2008-01-01T23:59:59.000Z

    required for ER-associated degradation. Nat Cell Biol, 3,Cue1p in ubiquitination and degradation at the ER surface.1999) Measuring protein degradation with green fluorescent

  2. Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies

    E-Print Network [OSTI]

    Croquette, Vincent

    Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies for protein folding studies and has been extensively stud- ied, both experimentally (at the ensemble level concentration. It is shown that new infor- mation about different aspects of the protein folding reaction can

  3. Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells

    SciTech Connect (OSTI)

    Nordlund, Henri R. [Department of Biological and Environmental Science, NanoScience Center, P.O. Box 35, FIN-40014 University of Jyvaeskylae (Finland)]. E-mail: henri.nordlund@uta.fi; Laitinen, Olli H. [AI Virtanen Institute, Department of Molecular Medicine, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio (Finland); Uotila, Sanna T.H. [Department of Biological and Environmental Science, NanoScience Center, P.O. Box 35, FIN-40014 University of Jyvaeskylae (Finland); Kulmala, Minna [Institute of Biotechnology, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FIN-00014 Helsinki (Finland); Kalkkinen, Nisse [Institute of Biotechnology, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FIN-00014 Helsinki (Finland); Kulomaa, Markku S. [Department of Biological and Environmental Science, NanoScience Center, P.O. Box 35, FIN-40014 University of Jyvaeskylae (Finland)

    2005-10-14T23:59:59.000Z

    The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins fused to it in insect cells. This fusion protein forms a unique tag, which serves as a multipurpose device enabling easy optimization of production, one-step purification via streptavidin-biotin interaction, and visualization of the fusion protein during downstream processing and in applications. In the present study, we demonstrate the successful production, purification, and detection of a natural rubber latex allergen Hev b5 with this system. We also describe the production of another NRL allergen with the system, Hev b1, which formed large aggregates and gave small yields in purification. The aggregates were detected at early steps by microscopical inspection of the infected insect cells producing this protein. Therefore, this fusion system can also be utilized as a fast indicator of the solubility of the expressed fusion proteins and may therefore be extremely useful in high-throughput expression approaches.

  4. Single-Molecule Fluorescence Studies of Protein Folding and Conformational Xavier Michalet,* Shimon Weiss, and Marcus Jager*

    E-Print Network [OSTI]

    Michalet, Xavier

    Single-Molecule Fluorescence Studies of Protein Folding and Conformational Dynamics Xavier Michalet. Single-Molecule Fluorescence Studies of Protein Folding and Conformations at Equilibrium 1796 4-Molecule Protein Folding under Nonequilibrium Conditions 1808 6. Conclusion 1809 7. Acknowledgments 1810 8

  5. Classification of Protein Localization Patterns Obtained via Fluorescence Light Microscopy

    E-Print Network [OSTI]

    Gordon, Geoffrey J.

    of the microscope. They should also be in- sensitive to the wide variability in cell morphology that is present in fluores- cence light microscope images of mammalian cells. Such images are generated on a regular basis by labeling one or more subcellular structures with fluorescent dyes and then collecting images

  6. Assaying protein import into mitochondria using fluorescence spectroscopy

    E-Print Network [OSTI]

    Cargill, Holly Beth

    2006-08-16T23:59:59.000Z

    of the Outer Membrane (TOM) complex, and the inner mitochondrial membrane (IM) via the Translocase of the Inner Membrane 23 (TIM23) complex. A novel system was set up to examine the import of matrix-targeted preproteins into mitochondria using fluorescence...

  7. Femtosecond lasing from a fluorescent protein in a one dimensional random cavity

    E-Print Network [OSTI]

    Drane, T M; Shapiro, M; Milner, V

    2015-01-01T23:59:59.000Z

    We present evidence of ultrafast random lasing from the fluorescent protein DsRed2 embedded in a random one-dimensional cavity. Lasing is achieved when a purified protein solution, placed inside a layered random medium, is optically excited with a femtosecond pump pulse in the direction perpendicular to the plane of random layers. We demonstrate that pumping with ultrashort pulses resulted in a lasing threshold two orders of magnitude lower than that found for nanosecond excitation.

  8. APPLICATION OF TEMPORAL TEXTURE FEATURES TO AUTOMATED ANALYSIS OF PROTEIN SUBCELLULAR LOCATIONS IN TIME SERIES FLUORESCENCE

    E-Print Network [OSTI]

    Gordon, Geoffrey J.

    are in constant movement within the cell, we extended our studies to time series images, which contain both to identify a protein's subcellular location is to label it with fluorescent dye, take microscope images this last step. The automated approach is more objective and sensitive than visual examination, and single

  9. Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport*S

    E-Print Network [OSTI]

    Campbell, Robert E.

    Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport*S Received concentrative nucleoside transporter, hCNT3, medi- ates Na /nucleoside and H /nucleoside co-transport. We describe a new approach to monitor H /uridine co-transport in cultured mammalian cells, using a p

  10. Use of green fluorescent protein for the analysis of protein-protein and protein-DNA interactions 

    E-Print Network [OSTI]

    Chen, Kai

    2011-01-01T23:59:59.000Z

    Restriction modification (RM) systems play a crucial role in preventing the entry of foreign DNA into the bacterial cell. The best studied Type I RM system is EcoKI from Escherichia coli K12. Both bacteriophage and ...

  11. Cyanine-based probe\\tag-peptide pair for fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana (Richland, WA); Cao, Haishi (Richland, WA)

    2010-08-17T23:59:59.000Z

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  12. ########## Green ###### x1. Green ######

    E-Print Network [OSTI]

    Shoj, Toshiaki

    ########## Green ###### # # # # x1. Green ###### Green ###, ### Fq ####### GLn(Fq) ######### 1955 # J.A. Green [G] ## ######. Green ########## (Green #########) ########, GLn(Fq) #############################. Green ######## GLn

  13. Development of rapid microbial methods for lysine quantification in feed ingredients based on green fluorescent protein fluorescence

    E-Print Network [OSTI]

    Chalova-Zhekova, Vesela I.

    2007-04-25T23:59:59.000Z

    -determination of bioavailable lysine in feedstuffs is important. An optical density (OD) based microbiological assay for lysine determination using E. coli lysine auxotroph has been previously developed. However, because the assay is based on bacterial growth response...

  14. Effect of enhanced Renilla luciferase and fluorescent protein variants on the Foerster distance of Bioluminescence resonance energy transfer (BRET)

    SciTech Connect (OSTI)

    Dacres, Helen, E-mail: helen.dacres@csiro.au [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)] [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia); Michie, Michelle; Wang, Jian [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)] [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia); Pfleger, Kevin D.G. [Laboratory for Molecular Endocrinology-GPCRs, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, The University of Western Australia, Perth (Australia)] [Laboratory for Molecular Endocrinology-GPCRs, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, The University of Western Australia, Perth (Australia); Trowell, Stephen C. [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)] [CSIRO Food Futures Flagship and Ecosystem Sciences, Canberra (Australia)

    2012-08-31T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer First experimental determination of Foerster distance (R{sub 0}) for enhanced BRET systems. Black-Right-Pointing-Pointer Effect of brighter BRET components RLuc2, RLuc8 and Venus was assessed. Black-Right-Pointing-Pointer Using brighter BRET components substantially increased (25%) R{sub 0} of the BRET{sup 1} system. Black-Right-Pointing-Pointer Using brighter BRET components marginally increased (2-9%) R{sub 0} of the BRET{sup 2} system. Black-Right-Pointing-Pointer Brighter BRET components improve the different weaknesses of BRET{sup 1} and BRET{sup 2} systems. -- Abstract: Bioluminescence resonance energy transfer (BRET) is an important tool for monitoring macromolecular interactions and is useful as a transduction technique for biosensor development. Foerster distance (R{sub 0}), the intermolecular separation characterized by 50% of the maximum possible energy transfer, is a critical BRET parameter. R{sub 0} provides a means of linking measured changes in BRET ratio to a physical dimension scale and allows estimation of the range of distances that can be measured by any donor-acceptor pair. The sensitivity of BRET assays has recently been improved by introduction of new BRET components, RLuc2, RLuc8 and Venus with improved quantum yields, stability and brightness. We determined R{sub 0} for BRET{sup 1} systems incorporating novel RLuc variants RLuc2 or RLuc8, in combination with Venus, as 5.68 or 5.55 nm respectively. These values were approximately 25% higher than the R{sub 0} of the original BRET{sup 1} system. R{sub 0} for BRET{sup 2} systems combining green fluorescent proteins (GFP{sup 2}) with RLuc2 or RLuc8 variants was 7.67 or 8.15 nm, i.e. only 2-9% greater than the original BRET{sup 2} system despite being {approx}30-fold brighter.

  15. ERK's Got Rhythm: Protein Oscillates in Breast Cells

    SciTech Connect (OSTI)

    None

    2009-11-01T23:59:59.000Z

    Researchers fluorescently-labeled the protein ERK and found it cycles between the nucleus and the cell proper of these breast cells over about 7 hours, causing them to pulsate green.

  16. This article was published as part of the 2009 Green Fluorescent Protein issue

    E-Print Network [OSTI]

    Jackson, Sophie

    and co-workers collected 85 000 jellyfish off Friday Harbor in Washington on the West Coast of North¨ quist, Secretary General of the Royal Swedish Academy of Sciences, spoke about ``the illuminating

  17. The extremely slow-exchanging core and acid-denatured state of green fluorescent protein

    E-Print Network [OSTI]

    Jackson, Sophie

    with a complex eleven- stranded -barrel structure. Previous studies have shown that it has a complex energy resonance ,,NMR... techniques to probe the energy landscape for folding of GFP in further detail. H a folding nucleus. Using a pulsed-labeling strategy, the acid-denatured state has been investigated

  18. Green fluorescent protein based indicators of dynamic redox changes and reactive oxygen species

    E-Print Network [OSTI]

    Dooley, Colette

    2006-01-01T23:59:59.000Z

    attached a singlet oxygen generator to the C-terminal ofencoded singlet oxygen generators, which is currentlyassays. A. Singlet oxygen generator and sensor are tethered;

  19. Near-infrared fluorescence glucose sensing based on glucose/galactose-binding protein coupled to 651-Blue Oxazine

    SciTech Connect (OSTI)

    Khan, Faaizah; Pickup, John C., E-mail: john.pickup@kcl.ac.uk

    2013-08-30T23:59:59.000Z

    Highlights: •We showed that the NIR fluorophore, 651-Blue Oxazine, is solvatochromic (polarity sensitive). •Blue Oxazine was covalently attached to mutants of glucose/galactose-binding protein (GBP). •Fluorescence intensity of GBP-Blue Oxazine increased with addition of glucose. •Fluorescence from bead-immobilised GBP-Blue Oxazine was detectable through skin in vitro. •This shows proof-of-concept for non-invasive glucose sensing using GBP-Blue Oxazine. -- Abstract: Near-infrared (NIR) fluorescent dyes that are environmentally sensitive or solvatochromic are useful tools for protein labelling in in vivo biosensor applications such as glucose monitoring in diabetes since their spectral properties are mostly independent of tissue autofluorescence and light scattering, and they offer potential for non-invasive analyte sensing. We showed that the fluorophore 651-Blue Oxazine is polarity-sensitive, with a marked reduction in NIR fluorescence on increasing solvent polarity. Mutants of glucose/galactose-binding protein (GBP) used as the glucose receptor were site-specifically and covalently labelled with Blue Oxazine using click chemistry. Mutants H152C/A213R and H152C/A213R/L238S showed fluorescence increases of 15% and 21% on addition of saturating glucose concentrations and binding constants of 6 and 25 mM respectively. Fluorescence responses to glucose were preserved when GBP-Blue Oxazine was immobilised to agarose beads, and the beads were excited by NIR light through a mouse skin preparation studied in vitro. We conclude GBP-Blue Oxazine shows proof-of-concept as a non-invasive continuous glucose sensing system.

  20. Measuring protein mobility by photobleaching GFP-chimeras in living cells

    E-Print Network [OSTI]

    Snapp, Erik Lee

    1 Measuring protein mobility by photobleaching GFP-chimeras in living cells Erik Snapp, Nihal Altan the fluorophore's fluorescence. The diffusive characteristics of green fluorescent protein (GFP)- chimeras, in which the same region of a cell expressing a GFP-chimera is repetitively photobleached and the loss

  1. The rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP

    SciTech Connect (OSTI)

    Pletnev, Sergei, E-mail: pletnevs@mail.nih.gov [Argonne National Laboratory, Argonne, IL 60439 (United States); Argonne National Laboratory, Argonne, IL 60439 (United States); Subach, Fedor V.; Verkhusha, Vladislav V. [Albert Einstein College of Medicine, Bronx, NY 10461 (United States); Dauter, Zbigniew, E-mail: pletnevs@mail.nih.gov [Argonne National Laboratory, Argonne, IL 60439 (United States)

    2014-01-01T23:59:59.000Z

    An analysis of the rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP is presented. The rotational order–disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order–disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.

  2. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Douglas C. (San Jose, CA); Silva, Christopher M. (Sunnyvale, CA); Bylina, Edward J. (San Jose, CA); Coleman, William J. (Moutain View, CA); Dilworth, Michael R. (Santa Cruz, CA); Yang, Mary M. (San Jose, CA)

    2002-09-24T23:59:59.000Z

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  3. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Dougalas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2003-12-09T23:59:59.000Z

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  4. Involvement of zeaxanthin and of the Cbr protein in the repair of photosystem II from photoinhibition in the green alga Dunaliella salina

    E-Print Network [OSTI]

    Polle, Jürgen

    photoinhibition in the green alga Dunaliella salina EonSeon Jin, Juergen E.W. Polle, Anastasios Melis * Department)-deficient mutant of the green alga Dunaliella salina (dcd1) showed an amplified response to irradiance stress low irradiance. The Cbr protein, known to accumulate when D. salina is exposed to irradiance stress

  5. Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity*

    E-Print Network [OSTI]

    Ikura, Mitsuhiko

    -0847, Japan, and the **Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, Brain Science Institute, RIKEN, Wako-city, Saitama 351-0198, Japan Yellow emission variants of green by creating regions of greater flexibility. F64L induced large conformational changes in the molecule, leading

  6. Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z.; Shemiakina, Irina I.; Chudakov, Dmitriy M.; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei (Russ. Acad. Sci.); (SAIC); (NCI)

    2012-08-10T23:59:59.000Z

    The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, {lambda}{sub ex} = 552 nm, {lambda}{sub em} = 578 nm) and its bright far-red fluorescent variant Katushka ({lambda}{sub ex} = 588 nm, {lambda}{sub em} = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 {angstrom} at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f.

  7. Geaux Green: GREEN GUIDE

    E-Print Network [OSTI]

    Harms, Kyle E.

    1 Geaux Green: GREEN GUIDE A GUIDE TO LIVING SUSTAINABLY #12;2 Table of Contents Geauxing Green p. 1-30 Transportation 3 Water 4 Energy 5 Food 6 Recycling 7 Around Baton Rouge p. 8 Other Ways To Be Green p. 9 #12;3 Geauxing Green: Transportation Takin' it easy Easy Streets has provided over 1,000 bike

  8. Engineering ESPT Pathways Based on Structural Analysis of LSSmKate Red Fluorescent Proteins with Large Stokes Shift

    SciTech Connect (OSTI)

    Piatkevich, K.; Malashkevich, V; Almo, S; Verkhusha, V

    2010-01-01T23:59:59.000Z

    LSSmKate1 and LSSmKate2 are monomeric red fluorescent proteins (RFPs) with large Stokes shifts (LSSs), which allows for efficient separation of absorbance and emission maxima, as well as for excitation with conventional two-photon laser sources. These LSSmKates differ by a single amino acid substitution at position 160 and exhibit absorbance maxima around 460 nm, corresponding to a neutral DsRed-like chromophore. However, excitation at 460 nm leads to fluorescence emission above 600 nm. Structures of LSSmKate1 and LSSmKate2, determined at resolutions of 2.0 and 1.5 {angstrom}, respectively, revealed that the predominant DsRed-chromophore configurations are cis for LSSmKate1 but trans for LSSmKate2. Crystallographic and mutagenesis analyses, as well as isotope and temperature dependences, suggest that an excited-state proton transfer (ESPT) is responsible for the LSSs observed in LSSmKates. Hydrogen bonding between the chromophore hydroxyl and Glu160 in LSSmKate1 and a proton relay involving the chromophore tyrosine hydroxyl, Ser158, and the Asp160 carboxylate in LSSmKate2 represent the putative ESPT pathways. Comparisons with mKeima LSS RFP suggest that similar proton relays could be engineered in other FPs. Accordingly, we mutated positions 158 and 160 in several conventional red-shifted FPs, including mNeptune, mCherry, mStrawberry, mOrange, and mKO, and the resulting FP variants exhibited LSS fluorescence emission in a wide range of wavelengths from 560 to 640 nm. These data suggest that different chromophores formed by distinct tripeptides in different environments can be rationally modified to yield RFPs with novel photochemical properties.

  9. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Purchasing & Technology Goals 6 & 7: Green Purchasing & Green Technology Our goal is to purchase and use environmentally sustainable products whenever possible and to implement...

  10. Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 1. Structural Characterization and Preliminary Application

    E-Print Network [OSTI]

    Boxer, Steven G.

    pH- sensitive dye, SNARF-1, to changes in pH were compared in the same cells. Results showVed August 9, 2002; ReVised Manuscript ReceiVed October 1, 2002 ABSTRACT: Novel dual emission, pH-sensitive cells by transient expression in PS120 fibroblasts. The responses of deGFP4 and a commercially available

  11. Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors. 2. Excited-State Dynamics

    E-Print Network [OSTI]

    Boxer, Steven G.

    of the high-energy absorption band centered at 398 nm and assigned to the neutral form of the chromophore-resolved emission dynamics and isotope effect appear to be very different from those of wild-type GFP [Chattoraj, M energy neutral chromophore to the lower energy intermediate anionic chromophore is achieved by proton

  12. Subpicosecond Protein Backbone Changes Detected during the Green-Absorbing Proteorhodopsin Primary Photoreaction

    E-Print Network [OSTI]

    zone and are responsible for a significant fraction of the biosphere's solar energy conversion.2 of Physics, Boston UniVersity, Boston, Massachusetts 02215, Department of Chemistry, Boston Uni. The protein changes we observe on a subpicosecond time scale may be involved in storage of the absorbed photon

  13. Engineering novel fluorescent proteins

    E-Print Network [OSTI]

    Shaner, Nathan Christopher

    2006-01-01T23:59:59.000Z

    aluminum block to prevent heating. Infrared and ultravioletHeating of plates was minimized by using a cold mirror to eliminate infrared

  14. Ultraviolet resonance Raman and fluorescence studies of folded and unfolded conformations of the membrane protein OmpA

    E-Print Network [OSTI]

    Sanchez, Katheryn Marie

    2010-01-01T23:59:59.000Z

    Thermodynamics of Membrane Protein Folding: Lessons from theA Model for Membrane Protein Folding”, H. S. Shafaat, K. M.goals of membrane protein folding studies is to ascertain

  15. Insight into the Common Mechanism of the Chromophore Formation in the Red Fluorescent Proteins: The Elusive Blue

    E-Print Network [OSTI]

    Krylov, Anna I.

    : The Elusive Blue Intermediate Revealed Ksenia B. Bravaya, Oksana M. Subach, Nadezhda Korovina, Vladislav V calculations identifying the nature of a blue intermediate, a key species in the process of the red chromophore formation in DsRed, TagRFP, fluorescent timers, and PAmCherry. The chromophore of the blue intermediate has

  16. Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green Purchasing Green Purchasing LANL is committed to purchasing and using environmentally preferable products. Contact Environmental Communication & Public Involvement P.O. Box...

  17. 428 BIOCHIMICAET BIOPHYSICAACTA pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE

    E-Print Network [OSTI]

    Govindjee

    428 BIOCHIMICAET BIOPHYSICAACTA BBA 46126 pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE on the "slow" (min) time course of Chlorophyll a fluorescence yield in the green alga Chlorella pyrenoidosa and in the blue-green alga Anacystis nidulans. In Chlorella, the decay of fluorescence yield, in the I- to 5-rain

  18. Millisecond timescale slimfield imaging and automated quantification of single fluorescent protein molecules for use in probing complex

    E-Print Network [OSTI]

    Leake, Mark C.

    protein (GFP),1 discovered in 1962 and now known to be utilized by many different ocean species which inhabit water depths where light struggles to penetrate. This natural form of GFP is excited maximally

  19. Buying green

    E-Print Network [OSTI]

    Bradshaw, William B., II

    2006-01-01T23:59:59.000Z

    Green development has received much attention over the past decade, with the greatest interest coming from designers. However, the development and investment communities have been slower to adopt green principles, and the ...

  20. Green Houston

    E-Print Network [OSTI]

    Spanjian, L.

    2012-01-01T23:59:59.000Z

    Laura Spanjian, Sustainability Director www.greenhoustontx.gov www.codegreenhouston.org www.houstongoc.org www.greenhoustontx.gov/houstondriveselectric Follow Houston, the green City greenhoustontx More information on all Green...

  1. A single amino acid change resulting in loss of fluorescence of eGFP in a viral fusion protein confers fitness and growth advantage to the recombinant vesicular stomatitis virus

    SciTech Connect (OSTI)

    Dinh, Phat X.; Panda, Debasis; Das, Phani B.; Das, Subash C.; Das, Anshuman [School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States) [School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States); The Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States); Pattnaik, Asit K., E-mail: apattnaik2@unl.edu [School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States); The Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States)

    2012-10-25T23:59:59.000Z

    Using a recombinant vesicular stomatitis virus encoding eGFP fused in-frame with an essential viral replication protein, the phosphoprotein P, we show that during passage in culture, the virus mutates the nucleotide C289 within eGFP of the fusion protein PeGFP to A or T, resulting in R97S/C amino acid substitution and loss of fluorescence. The resultant non-fluorescent virus exhibits increased fitness and growth advantage over its fluorescent counterpart. The growth advantage of the non-fluorescent virus appears to be due to increased transcription and replication activities of the PeGFP protein carrying the R97S/C substitution. Further, our results show that the R97S/C mutation occurs prior to accumulation of mutations that can result in loss of expression of the gene inserted at the G-L gene junction. These results suggest that fitness gain is more important for the recombinant virus than elimination of expression of the heterologous gene.

  2. TCD Green Campus Green Campus

    E-Print Network [OSTI]

    O'Mahony, Donal E.

    ) for the College. Where buildings or data are excluded from the scope of the system, this will be clearly indicated#12;DIAGRAM TCD Green Campus Policies Green Campus Committee 1. Energy, Climate Change & Greenhouse & EXCLUSIONS The scope of the TCD green flag application is comprehensive. All information for buildings

  3. Greening Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Transportation Goal 2: Greening Transportation LANL supports and encourages employees to reduce their personal greenhouse gas emissions by offering various commuting and work...

  4. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, Richard I. (Santa Fe, NM); Edwards, Bradley C. (Los Alamos, NM); Buchwald, Melvin I. (Santa Fe, NM); Gosnell, Timothy R. (Santa Fe, NM)

    1995-01-01T23:59:59.000Z

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement.

  5. SHORT COMMUNICATION The Chlorophyll Fluorescence Ratio F735/F700

    E-Print Network [OSTI]

    Gitelson, Anatoly

    sylvatica L.), elm (Ulmus minor 1988; Dahn et al., 1992). In green leaves, about 90% of Miller), and wild of reabsorption of the Chl fluorescence was quan- titatively estimated (Dahn et al., 1992; Gunther et al

  6. Angiographic Method Using Green Porphyrinew In Primmate Eyes

    DOE Patents [OSTI]

    Miller, Joan W. (Boston, MA); Young, Lucy H.Y. (Boston, MA); Gragoudas, Evangelos S. (Boston, MA)

    1998-01-13T23:59:59.000Z

    An angiographic method to observe the condition of blood vessels, including neovasculature in the eyes of living primates using green porphyrins and light at a wavelength of 550-700 nm to effect fluorescence is disclosed.

  7. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, R.I.; Edwards, B.C.; Buchwald, M.I.; Gosnell, T.R.

    1995-09-05T23:59:59.000Z

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement. 6 figs.

  8. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Design Andrew Bradbury Protein Engineering Geoff Waldo Structural Biology Tom Terwilliger LANL Facilities and Resources * Protein Crystallography Station: Scientists at this...

  9. GO GREEN! BUY GREEN! Introduction to Green Purchasing

    E-Print Network [OSTI]

    Lafferriere, Gerardo

    for Existing Buildings Sustainable purchasing policy Green Cleaning policy (with purchasing requirements) #12 ­ Building products ­ Green meetings and conference services · Low or non-toxic or non-hazardous chemicalsGO GREEN! BUY GREEN! Introduction to Green Purchasing April 22nd, 2010 Karen Preston - Purchasing

  10. Ultrafast Fluorescence Relaxation Spectroscopy of 6,7-Dimethyl-(8-ribityl)-lumazine and Riboflavin, Free and Bound to Antenna Proteins from Bioluminescent Bacteria

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Ultrafast Fluorescence Relaxation Spectroscopy of 6,7-Dimethyl-(8-ribityl)-lumazine and Riboflavin-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when

  11. Green Chemistry and Workers

    E-Print Network [OSTI]

    2009-01-01T23:59:59.000Z

    19. P. Anastas, J. Warner. 1998. Green Chemistry: Theory andto Advance New Science, Green Chemistry and EnvironmentalChronicle Extra: Guide to Green Jobs. Field with a Future.

  12. Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) Environmental AssessmentsGeoffrey(SC)Graphite Reactor 'In the-GreenGreen

  13. Greening Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.NewofGeothermal Heat AnnualGreen BusinessGreenGov

  14. Probing the denatured state ensemble with fluorescence

    E-Print Network [OSTI]

    Alston, Roy Willis

    2004-09-30T23:59:59.000Z

    around the original tryptophan substitutions in RNase Sa. Regardless of the denaturant, ?max for the proteins and model compounds differed very little, 349.3 ± 1.2 nm. However, significant differences were observed in the fluorescence intensity at ?max...

  15. Green Weight 

    E-Print Network [OSTI]

    Al-Haji, A.; Al-Omair, A.

    2010-01-01T23:59:59.000Z

    Green-weight is a novel system designed to supply a high-rise building with all its needs of electricity using wind energy and to supplement its lighting needs with natural sunlight. David Fisher has proposed an ingenious method to harness the wind...

  16. Folding Reporter Not 4 Profit BMTA LANL Agreement Number: LOS ALAMOS NATIONAL SECURITY, LLC

    E-Print Network [OSTI]

    , Berendzen J, Terwilliger TC (1999) "Rapid protein folding assay using Green Fluorescent Protein" Nat Biotech

  17. Folding Reporter Foreign Not 4 Profit BMTA LANL Agreement Number: LOS ALAMOS NATIONAL SECURITY, LLC

    E-Print Network [OSTI]

    , Berendzen J, Terwilliger TC (1999) "Rapid protein folding assay using Green Fluorescent Protein" Nat Biotech

  18. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Get Expertise Cliff Unkefer Director, Protein Crystallography Station Email Tom Terwilliger Laboratory Fellow Email Andrew Bradbury Bioscience Group Leader Email Rebecca...

  19. Green Manufacturing

    SciTech Connect (OSTI)

    Patten, John

    2013-12-31T23:59:59.000Z

    Green Manufacturing Initiative (GMI): The initiative provides a conduit between the university and industry to facilitate cooperative research programs of mutual interest to support green (sustainable) goals and efforts. In addition to the operational savings that greener practices can bring, emerging market demands and governmental regulations are making the move to sustainable manufacturing a necessity for success. The funding supports collaborative activities among universities such as the University of Michigan, Michigan State University and Purdue University and among 40 companies to enhance economic and workforce development and provide the potential of technology transfer. WMU participants in the GMI activities included 20 faculty, over 25 students and many staff from across the College of Engineering and Applied Sciences; the College of Arts and Sciences' departments of Chemistry, Physics, Biology and Geology; the College of Business; the Environmental Research Institute; and the Environmental Studies Program. Many outside organizations also contribute to the GMI's success, including Southwest Michigan First; The Right Place of Grand Rapids, MI; Michigan Department of Environmental Quality; the Michigan Department of Energy, Labor and Economic Growth; and the Michigan Manufacturers Technical Center.

  20. Thermo-Biolithography: A Technique for Patterning Nucleic Acids and Proteins

    E-Print Network [OSTI]

    Rubloff, Gary W.

    fluorescent protein (using tyrosinase-initiated conjugation). Because gelatin can be applied and removed under

  1. Green Purchasing & Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.NewofGeothermal Heat AnnualGreen Business infromPurchasing &

  2. Green Communities Grant Program

    Broader source: Energy.gov [DOE]

    Note: The Green Communities Grant Program is not currently accepting applications. The application deadline to receive a Green Communities designation was October 17, 2014. For designated...

  3. Green isoprene | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green isoprene Green isoprene Released: September 16, 2013 Predictive model a step toward bacteria being renewable fuel source A new transcriptomics-based model that accurately...

  4. Green Chemistry and Workers

    E-Print Network [OSTI]

    2009-01-01T23:59:59.000Z

    J. Warner. 1998. Green Chemistry: Theory and Practice. NewNew Science, Green Chemistry and Environmental Health.abstract.html 5. American Chemistry Council. 2003. Guide to

  5. Measurement and model assessment of fluorescence lifetime sensing in multiply scattering media

    E-Print Network [OSTI]

    Kuwana, Eddy

    2005-08-29T23:59:59.000Z

    fluorescent dyes, 3,3-diethylthiatricarbocyanine iodide (DTTCI) and Indocynanine Green (ICG), which exhibit distinctly different lifetimes and each exhibits single-exponential decay kinetics, were employed. Measurements of phase-modulation as a function...

  6. Green exciplex emission from a bilayer light-emitting diode containing a rare earth ternary complex

    E-Print Network [OSTI]

    Huang, Yanyi

    Green exciplex emission from a bilayer light-emitting diode containing a rare earth ternary complex form 18 October 2001 Abstract A bilayer organic light-emitting diode using a blue-fluorescent yttrium

  7. NEW ZEALAND'S NATIVE GREEN

    E-Print Network [OSTI]

    Canterbury, University of

    NEW ZEALAND'S NATIVE GREEN MISTLETOES What can you do to help? Many green mistletoe populations Zealand mistletoe species grow very slowly and take many years to replace lost branches. Why are green destruction and over-collecting are the biggest threats to green mistletoes. What can be done to save

  8. Proteins

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What's Possible forPortsmouth/Paducah47,193.70 HgPromisingProtectingSciTech Connect ProteinShop:

  9. Embedding your Green Message through Asynchronous Learning

    E-Print Network [OSTI]

    Filar Williams, Beth

    2011-01-01T23:59:59.000Z

    Conference: Going Green @Your library 2. Working Green,Greensboro Libraries, USA Sustainability, going green, eco-

  10. Fluorescent optical position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2005-11-15T23:59:59.000Z

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  11. Safe biodegradable fluorescent particles

    DOE Patents [OSTI]

    Martin, Sue I. (Berkeley, CA); Fergenson, David P. (Alamo, CA); Srivastava, Abneesh (Santa Clara, CA); Bogan, Michael J. (Dublin, CA); Riot, Vincent J. (Oakland, CA); Frank, Matthias (Oakland, CA)

    2010-08-24T23:59:59.000Z

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  12. GREEN'S FUNCTIONS OF VORTEX OPERATORS

    E-Print Network [OSTI]

    Polchinski, Joseph

    2013-01-01T23:59:59.000Z

    surface clustering the Green's function 5.1 actually spreadsb. Another graph for the same Green's function. c. Anothercomposite divergences. The Green's function 5.6, considered

  13. The Economics of Green Building

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2010-01-01T23:59:59.000Z

    Even among green buildings, increased energy efficiency isof total returns to energy efficient and green constructionof Energy and Indoor Environment Quality in Green Buildings:

  14. Blueprint for Greening Affordable Housing

    E-Print Network [OSTI]

    Ravega, Katea

    2010-01-01T23:59:59.000Z

    certification from the U.S. Green Building Council. Some ofthe book describes green building principles and how theyby decision makers. The green building characteristics

  15. The Economics of Green Building

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2010-01-01T23:59:59.000Z

    Environment Quality in Green Buildings: A Review," Nationalof Popular Attention to Green Building Notes: Sources:2007 - 2009 panel of green buildings and nearby control

  16. Coral fluorescence and symbiosis : photoacclimation, thermal shock, life history changes, and implications for reef monitoring

    E-Print Network [OSTI]

    Roth, Melissa Susan

    2010-01-01T23:59:59.000Z

    Diversity and evolution of coral fluorescent proteins. PLoSB. (2008). Climate change and coral reef bleaching: Anpigment in reef-building corals. Mar. Ecol. Prog. Ser. 364,

  17. REDUCING INCIDENTAL FLUORESCENCE IN LIVE CELL IMAGING A. Altinok 1

    E-Print Network [OSTI]

    California at Santa Barbara, University of

    , the technique is based on staining bio molecules selectively with proteins carrying fluorescence dyes of known. For instance, length measurements in Fig.1 would lack accuracy since the ends of the objects are blurry within

  18. Maroon, Gold .... and Green!Green!

    E-Print Network [OSTI]

    Minnesota, University of

    Definition Board of Regents Policy on Sustainability and Energy Efficiency (adopted July 2004) Sustainability of sustainability and energy efficient operations and a pioneering model among large, public research, land grantMaroon, Gold .... and Green!Green! Advancing Our Sustainability Efforts: University Sustainability

  19. Green Energy Tax Credit

    Broader source: Energy.gov [DOE]

    In addition to the Green Energy Tax Credit, the Carbon Tax Credit is available. This is the only carbon tax credit in the United States and it provides "certified green energy supply chain...

  20. Green Sturgeon, Longfin Smelt,

    E-Print Network [OSTI]

    Green Sturgeon, Longfin Smelt, and Dredging Operations in the San Francisco Estuary Summary Report, 2009. Summary Report: Green Sturgeon, Longfin Smelt, and Dredging in the San Francisco Estuary .......................................................... 8 4.1.2. Impacts of dredging

  1. Environmental Sustainability & Green Energy

    E-Print Network [OSTI]

    Denham, Graham

    Environmental Sustainability & Green Energy With escalating concerns about global energy shortages of carbon nanotubes for solar energy · ChemicalReactorEngineeringCentre: developing innovative green reactor contaminants in air, water and soil through advanced oxidation Environmental Remediation · Focus on sustainable

  2. Be Green, Be Aware, Be GreenAware

    E-Print Network [OSTI]

    Be Green, Be Aware, Be GreenAware © Experian 2007. All rights reserved. Confidential Green, Be Aware, Be GreenAware Overview Green is good; being aware is being responsible. With a projected worth of $500 billion for 20081 , the green marketplace is growing, as the environmental movement

  3. Staff Parking Crewe Green

    E-Print Network [OSTI]

    the petrol station and cross Macon Way. Walk down Crewe Green Road past the fire station and the Crewe campus

  4. Concentration in Green Design

    E-Print Network [OSTI]

    Shewchuk, Jonathan

    , energy, infrastructure or transport. Participants in this specialization area work closely with the GreenConcentration in Green Design Research and Education Opportunities Carnegie Mellon University Civil and Environmental Engineering www.ce.cmu.edu M.S. Concentration Green Design - Course Only Track As an extension

  5. Green's functions Lucia Reining

    E-Print Network [OSTI]

    Botti, Silvana

    Green's functions Lucia Reining Laboratoire des Solides Irradi´es Ecole Polytechnique, Palaiseau - France European Theoretical Spectroscopy Facility (ETSF) Belfast, 27.6.2007 Green's functions Lucia Motivation 2 Encouragement 3 Mathematics 4 Physics: the harmonic oscillator 5 Green's functions in quantum

  6. Green Bean Salad Ingredients

    E-Print Network [OSTI]

    Liskiewicz, Maciej

    Green Bean Salad Ingredients: 1 pound green beans 1/4 cup reserved liquid from beans 3 tablespoons Directions 1. Prepare green beans by snapping off ends, stringing and breaking into bite size pieces. 2 15 minutes. Drain and add to medium bowl, reserving 1/4 cup liquid. 3. While beans are cooking, cut

  7. Green Power Inverter Prvningsrapport

    E-Print Network [OSTI]

    Green Power Inverter Prøvningsrapport SolenergiCentret Søren Poulsen Ivan Katic Oktober 2004 #12;Green Power Inverter målerapport.doc SolenergiCentret - 04-03-2005 2 Forord Nærværende rapport indeholder Teknologisk Instituts bidrag til målinger i forbindelse med PSO projektet "Green Power Inverter

  8. Blue-green and green phosphors for lighting applications

    DOE Patents [OSTI]

    Setlur, Anant Achyut; Chandran, Ramachandran Gopi; Henderson, Claire Susan; Nammalwar, Pransanth Kumar; Radkov, Emil

    2012-12-11T23:59:59.000Z

    Embodiments of the present techniques provide a related family of phosphors that may be used in lighting systems to generate blue or blue-green light. The phosphors include systems having a general formula of: ((Sr.sub.1-zM.sub.z).sub.1-(x+w)A.sub.wCe.sub.x).sub.3(Al.sub.1-ySi.s- ub.y)O.sub.4+y+3(x-w)F.sub.1-y-3(x-w) (I), wherein 0fluorescent tubes, among others, to produce blue and blue/green light. Further, the phosphors may be used in blends with other phosphors, or in combined lighting systems, to produce white light suitable for illumination.

  9. The Green Computing Observatory: from

    E-Print Network [OSTI]

    Lefèvre, Laurent

    The Green Computing Observatory: from instrumentation to ontology Cécile Germain-Renaud1, Fredéric a gateway Files in XML format Available from the Grid Observatory portal GreenDays@LyonThe Green Computing) n GreenDays@LyonThe Green Computing Observatory #12;The GRIF-LAL computing room Green

  10. 2013 Green Tech 1 TECO Technology Foundation

    E-Print Network [OSTI]

    Kasahara, Hironori

    2013 Green Tech 1 TECO Technology Foundation #12;2013 Green Tech 2 #12;2013 Green Tech 3 Green Tech #12;2013 Green Tech 4 Green Tech #12;2013 Green Tech 5 Green Tech #12;2013 Green Tech 6 #12;2013 Green Tech 7 02/01 02/01-07/15 07/15 - 07

  11. Fluorescent Tube Lamps

    Broader source: Energy.gov [DOE]

    FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

  12. Bioindustry Creates Green Jobs

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bioindustry Creates Green Jobs Energy from abundant, renewable, domestic biomass can reduce U.S. dependence on oil, lower impacts on climate, and stimulate jobs and eco- nomic...

  13. NY Green Bank

    Energy Savers [EERE]

    Monday, October 6 th , 2014 Remarks by Nicholas Whitcombe, Managing Director, New York Green Bank Panel 1: Attracting and Maintaining Capital for Energy Transmission, Storage,...

  14. Green Power Offer (Maine)

    Broader source: Energy.gov [DOE]

    This chapter establishes requirements, standards and procedures and a competitive bidding process to implement the green power offer program. The program is designed to make renewable energy...

  15. Cooking with Green Beans

    E-Print Network [OSTI]

    Anding, Jenna

    2008-12-09T23:59:59.000Z

    This fact sheet describes the nutritional value and safe storage of green beans, a commodity food. It also offers food preparation ideas....

  16. The Green Gazette February 2013

    E-Print Network [OSTI]

    Harman, Neal.A.

    The Green Gazette February 2013 13 (number of Green Impact teams 2011/12) 27+ (number of Green Impact teams 2012/13) More than twice as many greening actions being carried out across campus and greater staff and student engagement with sustainability! Hello, welcome to the February Green Impact

  17. The Green Gazette January 2013

    E-Print Network [OSTI]

    Harman, Neal.A.

    The Green Gazette January 2013 It may seem like a drop in the ocean, but making sure all dripping rivalry, of course...): WISE Lab ­ WISE, Biosciences SEACAMS Lab ­ Biosciences Green There, Done That ­ Student Services Green Savers ­ IT Wales WISE Up ­ Go Green ­ WISE, Biosciences Green Power Rangers

  18. About Singapore Green Building Council

    E-Print Network [OSTI]

    About Singapore Green Building Council About SGBC Green Building Conference Conference Programme Green Building Conference In line with the mission of the Singapore Green Building Council (SGBC is please to present the inaugural SGBC Green Building Conference 2010 to be held from 13 ­ 16 September

  19. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen (Ann Arbor, MI); Sun, Yiru (Princeton, NJ); Giebink, Noel (Ann Arbor, MI); Thompson, Mark E. (Anaheim Hills, CA)

    2010-08-03T23:59:59.000Z

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  20. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen R. (Princeton, NJ); Sun, Yiru (Princeton, NJ); Giebink, Noel (Princeton, NJ); Thompson, Mark E. (Anaheim Hills, CA)

    2009-01-06T23:59:59.000Z

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  1. GREEN JOBS PROJECT Daniel Kammen

    E-Print Network [OSTI]

    Kammen, Daniel M.

    GREEN JOBS PROJECT Memo Daniel Kammen Meng Cai Karim El Alami December 2nd , 2014 #12;Contents.............................................................................................................................................3 1 Base of the Green jobs project per state ........................................................................................3 1.1 The Green jobs project from 2009

  2. Sandia Energy - A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Green Technology Home Energy Research EFRCs Solid-State Lighting Science EFRC A Green Technology A Green TechnologyTara Camacho-Lopez2015-05-11T21:08:32+00:00 Solid-State...

  3. GREEN BUILDINGS IN CHALLENGES AND

    E-Print Network [OSTI]

    Delaware, University of

    GREEN BUILDINGS IN DELAWARE: CHALLENGES AND OPPORTUNITIES FINAL REPORT A Renewable Energy-8405 Telefax: (302) 831-3098 Website: http://ceep.udel.edu #12;GREEN BUILDINGS IN DELAWARE: CHALLENGES .......................................................................................................2 2.1. Green Buildings

  4. Green Energy Act (Ontario, Canada)

    Broader source: Energy.gov [DOE]

    The Green Energy Act was created to expand Ontario's production of renewable energy, encourage energy conservation and promote the creation of clean-energy green jobs.

  5. GreenSlot: Scheduling Energy Consumption in Green Datacenters

    E-Print Network [OSTI]

    GreenSlot: Scheduling Energy Consumption in Green Datacenters Íñigo Goiri UPC/BSC and Rutgers Univ grid (as a backup). GreenSlot predicts the amount of solar energy that will be available in the near future, and schedules the workload to maximize the green energy consumption while meet- ing the jobs

  6. Green Light Pulse Oximeter

    DOE Patents [OSTI]

    Scharf, John Edward (Oldsmar, FL)

    1998-11-03T23:59:59.000Z

    A reflectance pulse oximeter that determines oxygen saturation of hemoglobin using two sources of electromagnetic radiation in the green optical region, which provides the maximum reflectance pulsation spectrum. The use of green light allows placement of an oximetry probe at central body sites (e.g., wrist, thigh, abdomen, forehead, scalp, and back). Preferably, the two green light sources alternately emit light at 560 nm and 577 nm, respectively, which gives the biggest difference in hemoglobin extinction coefficients between deoxyhemoglobin, RHb, and oxyhemoglobin, HbO.sub.2.

  7. Green Leasing Deployment Portfolio

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't YourTransport(Fact Sheet), Geothermal TechnologiesGeothermalGoGreat Green GasolineGreenGreen

  8. Fluorescent image tracking velocimeter

    DOE Patents [OSTI]

    Shaffer, Franklin D. (Library, PA)

    1994-01-01T23:59:59.000Z

    A multiple-exposure fluorescent image tracking velocimeter (FITV) detects and measures the motion (trajectory, direction and velocity) of small particles close to light scattering surfaces. The small particles may follow the motion of a carrier medium such as a liquid, gas or multi-phase mixture, allowing the motion of the carrier medium to be observed, measured and recorded. The main components of the FITV include: (1) fluorescent particles; (2) a pulsed fluorescent excitation laser source; (3) an imaging camera; and (4) an image analyzer. FITV uses fluorescing particles excited by visible laser light to enhance particle image detectability near light scattering surfaces. The excitation laser light is filtered out before reaching the imaging camera allowing the fluoresced wavelengths emitted by the particles to be detected and recorded by the camera. FITV employs multiple exposures of a single camera image by pulsing the excitation laser light for producing a series of images of each particle along its trajectory. The time-lapsed image may be used to determine trajectory and velocity and the exposures may be coded to derive directional information.

  9. Fluorescence analyzer for lignin

    SciTech Connect (OSTI)

    Berthold, J.W.; Malito, M.L.; Jeffers, L.

    1993-06-01T23:59:59.000Z

    An apparatus for measuring lignin concentration with time resolved fluorescence in an undiluted wood pulp or black liquor sample, on a real-time, in situ basis is described, comprising: light source means for applying excitation light pulses at a selected wavelength and at known time intervals to the undiluted sample for causing the lignin concentration to produce fluorescent emission light with a fluorescence intensity that monotonically decreases in a quenched fluorescence regime; light detector means for measuring the emission light at the known time intervals and establishing signals indicative thereof; switching means for turning said light detector means on at precise specified time intervals after each excitation light pulse; and signal processing means connected to the light source means and the light detector means for comparing intensities of the emission light from the lignin in the quenched fluorescence regime to the intensities of the excitation light pulses on a time resolved basis for providing a measurement of the lignin concentration in the undiluted sample as a function of the time resolved emission light intensity.

  10. Green Office Program: Innovation Credits

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    Green Office Program: Innovation Credits There are plenty of sustainable practices that aren't on our Green Office Program checklist. In an effort to encourage such practices, and reward offices Green: 5 Think outside the box, but make sure your innovation credits are approved by your Green Office

  11. Sustainable Stanford Greening Infrastructure & Choices

    E-Print Network [OSTI]

    ;Communication and Publication Sustainable Stanford website Year In Review Annual Report Cardinal Green

  12. International Trends in Green Building

    E-Print Network [OSTI]

    Zhang, Junshan

    International Trends in Green Building Friday, August 26, 2011 Registration 8:00 a.m. Presentation, Canada Green Building Council The presentation will cover international trends and innovations in the green-building industry as well as new opportunities for green-building collaboration with Arizona

  13. Green Beans with Garlic Ingredients

    E-Print Network [OSTI]

    Liskiewicz, Maciej

    Green Beans with Garlic Ingredients: 2 pounds green beans, fresh 1 teaspoon vegetable oil 4 cloves garlic 1/3 cup chicken broth 1/4 teaspoon pepper Directions: 1. Wash green beans to remove dirt. Snap off green beans until just tender, about 5 minutes. Drain and rinse with cold water to stop cooking. 3

  14. Green Energy Loans

    Broader source: Energy.gov [DOE]

    Illinois business owners, non-profit organizations, and local governments seeking loans for certain energy efficiency and renewable energy upgrades may apply for a rate reduction, under the Green...

  15. California's Green Economy

    E-Print Network [OSTI]

    existing materials Energy efficient product manufacturing, distribution, construction, installation, and maintenance Education, compliance and awareness Natural and sustainable product manufacturing For the complete's green economy Demand leads to pressure for additional supply of products and services Survey covers

  16. Green Supercomputing at Argonne

    ScienceCinema (OSTI)

    Pete Beckman

    2010-01-08T23:59:59.000Z

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing?everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently.

  17. Green Supercomputing at Argonne

    SciTech Connect (OSTI)

    Pete Beckman

    2009-11-18T23:59:59.000Z

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing—everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently.

  18. Green Chemistry COMMUNICATION

    E-Print Network [OSTI]

    California at Riverside, University of

    without expensive catalysts or solvents or complex process configurations. THF is a promising green solvent that is relatively non-toxic and miscible with water over a wide range of reaction con- ditions

  19. Green Supercomputing at Argonne

    ScienceCinema (OSTI)

    Beckman, Pete

    2013-04-19T23:59:59.000Z

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing?everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently. Argonne was recognized for green computing in the 2009 HPCwire Readers Choice Awards. More at http://www.anl.gov/Media_Center/News/2009/news091117.html Read more about the Argonne Leadership Computing Facility at http://www.alcf.anl.gov/

  20. Green's function on lattices

    E-Print Network [OSTI]

    Koushik Ray

    2014-09-27T23:59:59.000Z

    A method to calculate exact Green's functions on lattices in various dimensions is presented. Expressions in terms of generalized hypergeometric functions in one or more variables are obtained for various examples by relating the resolvent to a contour integral, evaluated using residues. Different ways of arranging the series leads to different combinations of hypergeometric functions providing identities involving generalized hypergeometric functions. The method is shown to be useful for computing Green's functions with next-nearest neighbor hopping as well.

  1. Green Supercomputing at Argonne

    SciTech Connect (OSTI)

    Beckman, Pete

    2009-01-01T23:59:59.000Z

    Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green supercomputing—everything from designing algorithms to use fewer kilowatts per operation to using cold Chicago winter air to cool the machine more efficiently. Argonne was recognized for green computing in the 2009 HPCwire Readers Choice Awards. More at http://www.anl.gov/Media_Center/News/2009/news091117.html Read more about the Argonne Leadership Computing Facility at http://www.alcf.anl.gov/

  2. Green Construction Codes 

    E-Print Network [OSTI]

    Blake, S.

    2011-01-01T23:59:59.000Z

    % Portfolio) ? Solar Powered Generators ? Hybrid Vehicle Fleet ? EV Charging Station Grid ? Reuse Warehouse ? Brownfields Municiple Setting Designations ? Waterworks Museum ? 1Million Trees / Community Gardens ? Green Office Challenge > 300... of the roof that is a rooftop garden, or green roof or covered by a rooftop deck covering 1/3 or less of the aggregate area of the roof ? Area including and adjacent to rooftop photovoltaic and solar thermal equipment, totaling not more than three...

  3. Multiphoton Add fluorescent dye

    E-Print Network [OSTI]

    Braun, Paul

    in toluene was prepared at 0.96 w/w. The monomer used was trimethylolpropane triacrylate (TMPTA) (Aldrich in toluene; the toluene was removed by rotovapping. For fluorescence imaging in colloidal crystals the electro-optic modulator is used to regulate the power of the beam, selectively exposing the regions

  4. Optical Shelving: Suppressed Fluorescence

    E-Print Network [OSTI]

    Mould, R A

    2007-01-01T23:59:59.000Z

    The shelving phenomenon of quantum optics, originally observed by Dehmelt, is analyzed in terms of the nRules that are given in another paper. The heuristic value of these rules is apparent because they reveal the mechanism that enforces the suppression of fluorescence during the dark period associated with shelving.

  5. Optical Shelving: Suppressed Fluorescence

    E-Print Network [OSTI]

    Richard A Mould

    2011-04-11T23:59:59.000Z

    The shelving phenomenon of quantum optics, originally observed by Dehmelt, is analyzed in terms of the qRules that are given in another paper. The heuristic value of these rules is apparent because they not only describe the dark period during shelving, but they reveal the mechanism that enforces the suppression of fluorescence during that time.

  6. The viscoelastic properties of chromatin and the nucleoplasm revealed by scale-dependent protein mobility

    E-Print Network [OSTI]

    Fabian Erdel; Michael Baum; Karsten Rippe

    2014-09-23T23:59:59.000Z

    The eukaryotic cell nucleus harbors the DNA genome that is organized in a dynamic chromatin network and embedded in a viscous crowded fluid. This environment directly affects enzymatic reactions and target search processes that access the DNA sequence information. However, its physical properties as a reaction medium are poorly understood. Here, we exploit mobility measurements of differently sized inert green fluorescent tracer proteins to characterize the viscoelastic properties of the nuclear interior of a living human cell. We find that it resembles a viscous fluid on small and large scales, but appears viscoelastic on intermediate scales that change with protein size. Our results are consistent with simulations of diffusion through polymers and suggest that chromatin forms a random obstacle network rather than a self-similar structure with fixed fractal dimension. By calculating how long molecules remember their previous position in dependence on their size, we evaluate how the nuclear environment affects search processes of chromatin targets.

  7. Fluorescence in nonlocal dissipative periodic structures

    E-Print Network [OSTI]

    Francesco Intravaia; Kurt Busch

    2015-02-24T23:59:59.000Z

    We present an approach for the description of fluorescence from optically active material embedded in layered periodic structures. Based on an exact electromagnetic Green's tensor analysis, we determine the radiative properties of emitters such as the local photonic density of states, Lamb shifts, line widths etc. for a finite or infinite sequence of thin alternating plasmonic and dielectric layers. In the effective medium limit, these systems may exhibit hyperbolic dispersion relations so that the large wave-vector characteristics of all constituents and processes become relevant. These include the finite thickness of the layers, the nonlocal properties of the constituent metals, and local-field corrections associated with an emitter's dielectric environment. In particular, we show that the corresponding effects are non-additive and lead to considerable modifications of an emitter's luminescence properties.

  8. Renewable Surface Fluorescence Sandwich Immunoassay Biosensor...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Renewable Surface Fluorescence Sandwich Immunoassay Biosensor for Rapid Sensitive Botulinum Toxin Detection in an Automated Renewable Surface Fluorescence Sandwich Immunoassay...

  9. Single-molecule Fluorescence Spectroelectrochemistry of Cresyl...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fluorescence Spectroelectrochemistry of Cresyl Violet. Abstract: We coupled scanning fluorescence microscopy with a potentiostat via a three-electrode electrochemical...

  10. Green Industrial Policy: Trade and Theory

    E-Print Network [OSTI]

    Karp, Larry; Stevenson, Megan

    2012-01-01T23:59:59.000Z

    promote the development of green energy, and thus qualify asa rapid growth in green energy capacity in these countriesfastest growing sources of green energy, still make up less

  11. High Performance Green Schools Planning Grants

    Broader source: Energy.gov [DOE]

    The Governor's Green Government Council of Pennsylvania provides an incentive for new schools to be built according to green building standards. High Performance Green Schools Planning Grants are...

  12. Green Industrial Policy: Trade and Theory

    E-Print Network [OSTI]

    Karp, Larry; Stevenson, Megan

    2012-01-01T23:59:59.000Z

    Papers Year 2012 Paper 1126 Green Industrial Policy: Trade© 2012 by author(s). Green Industrial Policy: Trade andreality and the potential for green indus- trial policy. We

  13. Green Libraries Are More Than Just Buildings

    E-Print Network [OSTI]

    Aulisio, George J.

    2013-01-01T23:59:59.000Z

    United States. Electronic Green Journal, Issue 35, Earth Daymake their library a “green library. ” References Antonelli,M. (2008). The Green library movement: an overview and

  14. Green Libraries Are More Than Just Buildings

    E-Print Network [OSTI]

    Aulisio, George J.

    2013-01-01T23:59:59.000Z

    France: UNESCO. U.S. Green Building Council (USGBC). (internationally recognized green building program. Retrievednecessarily entail a green building, but it does involve a

  15. Green Libraries Are More Than Just Buildings

    E-Print Network [OSTI]

    Aulisio, George J.

    2013-01-01T23:59:59.000Z

    make their library a “green library. ” References Antonelli,M. (2008). The Green library movement: an overview andEducation. (2013). Green libraries on campus. Retrieved from

  16. Green Industrial Policy: Trade and Theory

    E-Print Network [OSTI]

    Karp, Larry; Stevenson, Megan

    2012-01-01T23:59:59.000Z

    green industries, low cost green imports from China might beobjecting to low cost green imports from China. Industrialinfrastructure and licensing costs. China leads the world in

  17. The Air-Fluorescence Yield

    E-Print Network [OSTI]

    F. Arqueros; F. Blanco; D. Garcia-Pinto; M. Ortiz; J. Rosado

    2008-07-30T23:59:59.000Z

    Detection of the air-fluorescence radiation induced by the charged particles of extensive air showers is a well-established technique for the study of ultra-high energy cosmic rays. Fluorescence telescopes provide a nearly calorimetric measure of the primary energy. Presently the main source of systematic uncertainties comes from our limited accuracy in the fluorescence yield, that is, the number of fluorescence photons emitted per unit of energy deposited in the atmosphere by the shower particles. In this paper the current status of our knowledge on the fluorescence yield both experimental an theoretical will be discussed.

  18. Fluorescence Assay 2. http://www.tgrbio.com/cancer-cell-lines-primary-cell-

    E-Print Network [OSTI]

    Collins, Gary S.

    hazardous waste. Fluorescence microscopy is a powerful tool with a broad scope of applications easily fluorescence microscopy. In creating an innovative protocol for the 5-HT3a receptor, it is necessary-HT3a Expression Purification/Characterization While creating a protocol for protein expression

  19. Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit

    E-Print Network [OSTI]

    Agmon, Noam

    Transition in the Temperature-Dependence of GFP Fluorescence: From Proton Wires to Proton Exit protein, photo-excitation leads to excited-state proton transfer from its chromophore, leaving behind a strongly fluorescing anion, while the proton is commonly thought to migrate internally to Glu-222. X

  20. Volume 57, number 3 FEBS LETTERS October 1975 INTERACTIONS OF FLUORESCENT ANALOGS OF ADENINE NUCLEOTIDES

    E-Print Network [OSTI]

    Govindjee

    NUCLEOTIDES WITH COUPLING FACTOR PROTEIN ISOLATED FROM SPINACH CHLOROPLASTS David L. VANDERMEULEN by Farron [ 151, was used in determining its molarity. The fluorescent nucleotides 1,N6ethenoadenosine mono fluorescent nucleotides (mol. wt. G600) bind to the relatively large (mol. wt. = 325 000), slowly rotating CFI

  1. The Structure of a Cyanobacterial Bicarbonate Transport Protein...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cyanobacterial Bicarbonate Transport Protein, CmpA. Abstract: Cyanobacteria, blue-green algae, are the most abundant autotrophs in aquatic environments and form the base of the...

  2. Magnetic fluorescent lamp

    DOE Patents [OSTI]

    Berman, S.M.; Richardson R.W.

    1983-12-29T23:59:59.000Z

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  3. Mechanism of the efficient quenching of tryptophan fluorescence in human gamma crystallin

    E-Print Network [OSTI]

    Chen, Jiejin, Ph. D. Massachusetts Institute of Technology

    2008-01-01T23:59:59.000Z

    Quenching of the fluorescence of buried tryptophans (Trps) is an important reporter of protein conformation. Human [gamma]D-crystallin (H[gamma]D-Crys) and human [gamma]S-crystallin (H[gamma]S-Crys) are both very stable ...

  4. Computational image analysis of subcellular dynamics in time-lapse fluorescence microscopy

    E-Print Network [OSTI]

    Huang, Austin V., 1980-

    2005-01-01T23:59:59.000Z

    The use of image segmentation and motion tracking algorithms was adapted for analyzing time-lapse data of cells with fluorescently labeled protein. Performance metrics were devised and algorithm parameters were matched to ...

  5. NIST Presents Green Button: Intro

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29T23:59:59.000Z

    Part 1/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  6. NIST Presents Green Button: Policy

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29T23:59:59.000Z

    Part 2/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  7. NIST Presents Green Button: Technical

    ScienceCinema (OSTI)

    Wollman, David

    2013-05-29T23:59:59.000Z

    Part 3/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  8. NIST Presents Green Button: Policy

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01T23:59:59.000Z

    Part 2/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  9. NIST Presents Green Button: Technical

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01T23:59:59.000Z

    Part 3/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  10. NIST Presents Green Button: Intro

    SciTech Connect (OSTI)

    Wollman, David

    2012-01-01T23:59:59.000Z

    Part 1/3 of NIST's presentation about the Green Button program. Slides available at http://energy.gov/developer.

  11. GREEN HOMES LONG ISLAND

    E-Print Network [OSTI]

    Kammen, Daniel M.

    energy bill, reduce your carbon footprint... at little or no cost to you. #12;A Message From Supervisor energy-efficient and reduce our community's carbon footprint. Why do we call it Long Island Green Homes to yourevery day. By making basic improvements to yourevery day home, you can reduce your carbon footprint

  12. The Sustainability "Green Initiative"

    E-Print Network [OSTI]

    Harms, Kyle E.

    sustainability approach aimed at: ·Lowering energy use and cost ·Reducing greenhouse gases ·Water and waste waterThe Sustainability "Green Initiative" At LAPPA October, 2008 #12;Sustainability is the capacity ·Carbon Footprint ·Ozone Friendly ·Global Warming ·Renewable Energy ·Biodegradable What does this whole

  13. Amplifying Fluorescent Polymers Direct Synthesis of an OligonucleotidePoly-

    E-Print Network [OSTI]

    Tan, Weihong

    Amplifying Fluorescent Polymers Direct Synthesis of an Oligonucleotide­Poly- (phenylene ethynylene bioprobes can be constructed with these polymers for selective target recog- nition. To achieve this objective, the polymer must be conjugated with a biomolecule such as a DNA strand, a peptide, or a protein

  14. Introduction to Green Building & LEED

    E-Print Network [OSTI]

    Zaferatos, Nicholas C.

    Introduction to Green Building & LEED Alistair Jackson Principal O'Brien & Company alistair ­ Green Building Standard · MLS Listing Service #12;#12;#12;#12;#12;#12;#12;#12 of existing infrastructure and provides a foundation for high performance green buildings · Integrated

  15. Bowling Green State University Institutional

    E-Print Network [OSTI]

    Moore, Paul A.

    Bowling Green State University Institutional Animal Care and Use Committee Policy/Procedure Manual animal research at Bowling Green State University. Reading over the first three policies will provide #12;309A University Hall Bowling Green, OH 43403-0183 Phone 419-372-7716 Fax 419-372-6916 email hsrb

  16. City of Chicago- Green Power Purchasing

    Broader source: Energy.gov [DOE]

    For more green power purchasing program listings, visit the U.S. Department of Energy Green Power Network.

  17. Ghana Green Building Council public launch

    E-Print Network [OSTI]

    Ghana Green Building Council public launch examples of green buildings in South Africa eric noir, WSP GBD 17 August 2011 #12;Ghana Green Building Council public launch | examples of green buildings in South Africa GREEN by DESIGN PLATINUM GOLD MAJORSPONSORS #12;Ghana Green Building Council public launch

  18. Structural model and spectroscopic characteristics of the FMO antenna protein from the aerobic chlorophototroph, Candidatus Chloracidobacterium thermophilum

    SciTech Connect (OSTI)

    Wen, Jianzhong; Tsukatani, Yusuke; Cui, Weidong; Zhang, Hao; Gross, Michael L; Bryant, Donald A; Blankenship, R. E.

    2011-01-01T23:59:59.000Z

    The Fenna–Matthews–Olson protein (FMO) binds seven or eight bacteriochlorophyll a (BChl a) molecules and is an important model antenna system for understanding pigment-protein interactions and mechanistic aspects of photosynthetic light harvesting. FMO proteins of green sulfur bacteria (Chlorobiales) have been extensively studied using a wide range of spectroscopic and theoretical approaches because of their stability, the spectral resolution of their pigments, their water-soluble nature, and the availability of high-resolution structural data. We obtained new structural and spectroscopic insights by studying the FMO protein from the recently discovered, aerobic phototrophic acidobacterium, Candidatus Chloracidobacterium thermophilum. Native C. thermophilum FMO is a trimer according to both analytical gel filtration and native-electrospray mass spectrometry. Furthermore, the mass of intact FMO trimer is consistent with the presence of 21–24 BChl a in each. Homology modeling of the C. thermophilum FMO was performed by using the structure of the FMO protein from Chlorobaculum tepidum as a template. C. thermophilum FMO differs from C. tepidum FMO in two distinct regions: the baseplate, CsmA-binding region and a region that is proposed to bind the reaction center subunit, PscA. C. thermophilum FMO has two fluorescence emission peaks at room temperature but only one at 77 K. Temperature-dependent fluorescence spectroscopy showed that the two room-temperature emission peaks result from two excited-state BChl a populations that have identical fluorescence lifetimes. Modeling of the data suggests that the two populations contain 1–2 BChl and 5–6 BChl a molecules and that thermal equilibrium effects modulate the relative population of the two emitting states.

  19. STORM/PALM - Super Resolution Fluorescence Microscope | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    STORMPALM - Super Resolution Fluorescence Microscope STORMPALM - Super Resolution Fluorescence Microscope EMSL has developed and offers Fluorescence, Super Resolution STORM...

  20. Fluorescence analyzer for lignin

    SciTech Connect (OSTI)

    Berthold, John W. (Salem, OH); Malito, Michael L. (Hubbard, OH); Jeffers, Larry (Alliance, OH)

    1993-01-01T23:59:59.000Z

    A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

  1. Fluorescent temperature sensor

    DOE Patents [OSTI]

    Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

    2009-03-03T23:59:59.000Z

    The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

  2. Identification of Nucleotides with Identical Fluorescent Labels Based on Fluorescence

    E-Print Network [OSTI]

    Myrick, Michael Lenn

    Identification of Nucleotides with Identical Fluorescent Labels Based on Fluorescence Polarization for discriminating among the four DNA nucleotides la- beled identically with tetramethylrhodamine is described and demonstrated. Labeled nucleotides were dissolved in buffered surfactant solutions. In room temperature 4.5 m

  3. Quantitative microscopic spectral fluorescence measurement of crude oil, bitumen, kerogen, and coal

    SciTech Connect (OSTI)

    Mukhopadhyay, P.K.; Rullkoetter, J.

    1986-05-01T23:59:59.000Z

    Ten samples each of black shale (kerogen and bitumen fractions) from Lias epsilon, coal from Western Canada and nine crude oil and condensate samples from Alaska and northern Germany have been studied using quantitative microscopic spectral fluorescence. The parameters used are lambda/sub max/, red/green quotient (Q), and alteration of fluorescence emission intensity under UV excitation. Using the same parameters, the data show that kerogen and crude oil have opposite maturation trends. Autochthonous bitumens include both kerogen and crude oil characters. Immature, biodegraded, or normal crude oil of different maturity can be characterized using these parameters. Quantitative spectral fluorescence microscopy yields more accurate maturation parameters for the Type I and II kerogens than vitrinite reflectance because the fluorescence of liptinites are used (i.e., the main oil-generating macerals). This method may become the most suitable inexpensive scanning technique for the characterization of crude oil, condensate, and autochthonous/allochthonous source rock bitumens.

  4. LED-Induced Fluorescence System for Tea Classification and Quality Assessment

    E-Print Network [OSTI]

    Dong, Yongjiang; Mei, Liang; Feng, Chao; Yan, Chunsheng; He, Sailing

    2013-01-01T23:59:59.000Z

    A fluorescence system is developed by using several light emitting diodes (LEDs) with different wavelengths as excitation light sources. The fluorescence detection head consists of multi LED light sources and a multimode fiber for fluorescence collection, where the LEDs and the corresponding filters can be easily chosen to get appropriate excitation wavelengths for different applications. By analyzing fluorescence spectra with the principal component analysis method, the system is utilized in the classification of four types of green tea beverages and two types of black tea beverages. Qualities of the Xihu Longjing tea leaves of different grades, as well as the corresponding liquid tea samples, are studied to further investigate the ability and application of the system in the evaluation of classification/quality of tea and other foods.

  5. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01T23:59:59.000Z

    the larger diffusion of green and energy efficient buildingsowners, the costs of green and energy efficient buildings,market. Demand for Green and Energy Efficient Buildings The

  6. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01T23:59:59.000Z

    Doing Well by Doing Good? Green Office Buildings. AmericanWhy Do Companies Rent Green? Real Property and Corporatepaper? ] Kahn, Matthew. 2006. “Green Cities, Urban Grown and

  7. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01T23:59:59.000Z

    owners, the costs of green and energy efficient buildings,the larger diffusion of green and energy efficient buildingsmarket. Demand for Green and Energy Efficient Buildings The

  8. Green Buildings in Green Cities: Integrating Energy Efficiency into the Real Estate Industry

    E-Print Network [OSTI]

    Bardhan, Ashok; Kroll, Cynthia A.

    2011-01-01T23:59:59.000Z

    Costs and Financial Benefits of Green Buildings” A Report toEvidence on the Green Building Rent and Price Premium,” (Properties. San Rafael: Green Building Finance Consortium.

  9. INL Green Building Strategy

    SciTech Connect (OSTI)

    Jennifer Dalton

    2005-05-01T23:59:59.000Z

    Green buildings, also known as sustainable buildings, resource efficient buildings, and high performance buildings, are structures that minimize the impact on the environment by using less energy and water, reducing solid waste and pollutants, and limiting the depletion of natural resources. As Idaho National Laboratory (INL) becomes the nation’s premier nuclear energy research laboratory, the physical infrastructure will be established to help accomplish the mission. This infrastructure, particularly the buildings, should incorporate green design features in order to be environmentally responsible and reflect an image of progressiveness and innovation to the public and prospective employees. With this in mind, the recommendations described in this strategy are intended to form the INL foundation for green building standards. The recommendations in this strategy are broken down into three levels: Baseline Minimum, Leadership in Energy and Environmental Design (LEED)Certification, and Innovative. Baseline Minimum features should be included in all new occupied buildings no matter what the purpose or size. These features do not require significant research, design, or capital costs and yet they can reduce Operation and Maintenance (O&M) costs and produce more environmentally friendly buildings. LEED Certification features are more aggressive than the Baseline Minimums in that they require documentation, studies, and/or additional funding. Combined with the Baseline Minimums, many of the features in this level will need to be implemented to achieve the goal of LEED certification. LEED Silver certification should be the minimum goal for all new buildings (including office buildings, laboratories, cafeterias, and visitor centers) greater than 25,000 square feet or a total cost of $10 million. Innovative features can also contribute to LEED certification, but are less mainstream than those listed in the previous two levels. These features are identified as areas where INL can demonstrate leadership but they could require significant upfront cost, additional studies, and/or development. Appendix A includes a checklist summary of the INL Green Building Strategy that can be used as a tool during the design process when considering which green building features to include. It provides a quick reference for determining which strategies have lower or no increased capital cost, yield lower O&M costs, increase employee productivity, and contribute to LEED certification.

  10. Assignment of the tyrosinase-related protein-2 gene (TYRP2) to human chromosome 13q31-q32 by fluorescence in situ hybridization: Extended synteny with mouse chromosome 14

    SciTech Connect (OSTI)

    Sturm, R.A. (Univ. of Queensland, Brisbane (Australia)); Baker, E.; Sutherland, G.R. (Centre for Medical Genetics, North Adelaide (Australia))

    1994-05-01T23:59:59.000Z

    A recombinant human genomic liver DNA [lambda]-phage library was screened with the insert of the pHuTRP-2 cDNA clone to isolate a series of bacteriophage with inserts spanning the human TYRP2 gene. One of the [lambda]-phage clones ([lambda]HuT-YRP2-7) containing a 2-kb HindIII fragment with the 5[prime] exon sequence of the cDNA as determined by sequence analysis was used for the gene localization study. DNA prepared from the phage by Qiagen chromatography was nick-translated with biotin-14-dATP and hybridized in situ at a final concentration of 5 ng/[mu]l to metaphases from two normal males. The fluorescence in situ hybridization method was modified from that previously described in that chromosomes were stained before analysis with both propidium iodide as counterstain and DAPI for chromosome identification. Twenty metaphases from the first normal male were examined for fluorescent signal. All of these metaphases showed signal on one or both chromatids of chromosome 13 in the region 13q31-q33; 88% of this signal was at the interface of bands 13q31-q32. There was a total of four nonspecific background dots observed in these 20 metaphases. A similar result was obtained from hybridization of the probe to 20 metaphases from the second normal male (data not shown). This region has also been shown to contain the propionyl coenzyme A carboxylase [alpha]-chain gene by in situ hybridization. The localization of the TYRP2 locus to human chromosome 13q31-q32 extends the syntenic region of chromosome 13 with mouse chromosome 14. 15 refs., 1 fig.

  11. Home Articles Directions Contact Green Resources & Links The Green Building Gallery Gill Holland, green developer / film producer

    E-Print Network [OSTI]

    Home Articles Directions Contact Green Resources & Links The Green Building Gallery Gill Holland to try to follow the US Green Building Council's (USGBC) guidelines for both green building building and try to bring aw areness of green building to Louisville and its developers. After making tw o

  12. Commercial & Institutional Green Building Performance

    E-Print Network [OSTI]

    Harrison, S.; Mundell,C.; Meline, K.; Kraatz,J.

    2014-01-01T23:59:59.000Z

    Buildings Voluntary Green Building Programs: • LEED www.usgbc.org • Living Building Challenge living-future.org/lbc • Green Globes www.greenglobes.com • WELL Buildings wellbuildinginstitute.com • ENERGY STAR energystar.gov ESL-KT-14...The North Central Branch Texas Public Works Association Commercial & Institutional Green Building Performance 11.19.2014 ESL-KT-14-11-26 CATEE 2014: Clean Air Through Efficiency Conference, Dallas, Texas Nov. 18-20 Q&A Your Presenters: Chris...

  13. Nuclear Resonance Fluorescence for Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian

    2010-01-01T23:59:59.000Z

    clandestine material with nuclear resonance fluorescence”.E. Norman, UC Berkeley Dept. of Nuclear Engineering, privatepp. 349. G. Warren et al. “Nuclear Resonance Fluorescence of

  14. redD as a Reporter for Transcriptional Activity 551 Application of redD, the Transcriptional Activator Gene

    E-Print Network [OSTI]

    Bibb, Mervyn

    glaucescens, which encodes tyrosinase (Paget et al., 1994), and the EGFP gene for green-fluorescent protein

  15. The Economics of Green Building

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2010-01-01T23:59:59.000Z

    Green Building Piet Eichholtz Maastricht University Netherlands p.eichholtz@maastrichtuniversity.nl Nils Kok Maastricht University Netherlands n.kok@maastrichtuniversity.nl Abstract Research on climate change

  16. Innovative Financing for Green Infrastructure

    Office of Energy Efficiency and Renewable Energy (EERE)

    Topic OverviewFinancing green infrastructure is critical to taking projects from planning to implementation and beyond, including sustaining operations and maintenance. This 90-minute webcast will...

  17. Frederick County- Green Building Program

    Broader source: Energy.gov [DOE]

    Frederick County administers a green building program. It has two goals: (1) to ensure that County building projects implement strategies that enhance environmental performance and fiscal...

  18. Go Green Fund (Saskatchewan, Canada)

    Broader source: Energy.gov [DOE]

    The Go Green Fund is a financial commitment from the Government of Saskatchewan to assist Saskatchewan's people, communities, non-government organizations and businesses address the province's most...

  19. Industrial Green | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn Other News link to facebook link to04948Industrial Green

  20. Global green building Presenter: Jane Henley

    E-Print Network [OSTI]

    Global green building Presenter: Jane Henley Chief Executive Officer World Green Building Council worldwide ·10% plus of global gross domestic product #12;World Green Building Council Growth #12;World Green Building Council #12;World Green Building Council Regional networks #12;· Champions the GBC model

  1. Fluorescent fluid interface position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2004-02-17T23:59:59.000Z

    A new fluid interface position sensor has been developed, which is capable of optically determining the location of an interface between an upper fluid and a lower fluid, the upper fluid having a larger refractive index than a lower fluid. The sensor functions by measurement, of fluorescence excited by an optical pump beam which is confined within a fluorescent waveguide where that waveguide is in optical contact with the lower fluid, but escapes from the fluorescent waveguide where that waveguide is in optical contact with the upper fluid.

  2. Red phosphors for use in high CRI fluorescent lamps

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

    2005-11-15T23:59:59.000Z

    Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

  3. Sandia National Laboratories: A Green Technology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ClimateA Green Technology A Green Technology videobanner Solid-State Lighting: a New Green Technology S peaker: Jerry Simmons (EFRC Director) and Mike Coltrin (EFRC Co-Director)...

  4. GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS

    E-Print Network [OSTI]

    Shoj, Toshiaki

    GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS University of Tokyo Noda, Chiba 278-8510, Japan Abstract. Green functions called symbols. Generali* *zing this, we define Green functions associated to complex reflection

  5. Green Industrial Policy: Trade and Theory

    E-Print Network [OSTI]

    Karp, Larry; Stevenson, Megan

    2012-01-01T23:59:59.000Z

    have to be applied to green energy, in order to move towardpromote the development of green energy, and thus qualify asa rapid growth in green energy capacity in these countries

  6. Building Environmental Sustainability Bowling Green State University

    E-Print Network [OSTI]

    Moore, Paul A.

    Building Environmental Sustainability at Bowling Green State University Executive Summary Bud Folkins to offer recommendations for building an environmentally sustainable community at Bowling Green Services September 2002 #12;ii Introduction Bowling Green State University can become the premier public

  7. BRANCHED ALKANES FROM BLUE-GREEN ALGAE

    E-Print Network [OSTI]

    Han, Jerry; Calvin, Melvin.

    2008-01-01T23:59:59.000Z

    ALKANES FROM BLUE-GREEN ALGAE RECEIV r -· LAWREW RADIATIONAlkanes From Blue-Green Algae by Jerry Han and Oep~rtment l~alkanes from blue-green algae were separated on a The

  8. BRANCHED ALKANES FROM BLUE-GREEN ALGAE

    E-Print Network [OSTI]

    Han, Jerry; Calvin, Melvin.

    2008-01-01T23:59:59.000Z

    ~ BRANCHED ALKANES FROM BLUE-GREEN ALGAE RECEIV r -· LAWREWBranched Alkanes From Blue-Green Algae by Jerry Han and Oep~Branched alkanes from blue-green algae were separated on a

  9. WEBSITE RESOURCES FOR GREEN JOBS

    E-Print Network [OSTI]

    Edwards, Paul N.

    of worldwide environmental jobs http://www.justmeans.com/index.php?action=jobsearch ­ List of environmentally jobs in the U.S. & worldwide http://www.sustainlane.com/greenjobs ­ Online community for green Green Jobs Task Force http://www.jobsinbiofuels.com/ ­ Ethanol and biofuel jobs http

  10. Going for "Green" Sustainable Building

    E-Print Network [OSTI]

    Going for "Green" Sustainable Building Certification Statistics Europe Status May 2011 rics.org/sustainability #12;Sustainable Building Certification Statistics Europe Foreword Investors are increasingly the usefulness of sustainability certificates in mainstreaming the uptake of so-called `green' buildings across

  11. GREEN GUIDE MAISON AM GROND

    E-Print Network [OSTI]

    van der Torre, Leon

    For this task, we took the ideas from Zihomara Matar. Author of the ECO2 practice guides. Task 4 ­ Laundry: GoodGREEN GUIDE MAISON AM GROND One Earth... Once Chance Team Members: Jose Miguel Lopez Becerra ­ Green Guide Task 1 ­ Garbage Separation This task is related to explain the different types of garbage

  12. Greening Brownfield Properties Jennifer Casler

    E-Print Network [OSTI]

    Edwards, Paul N.

    Greening Brownfield Properties by Jennifer Casler Yang Chen Joshua Cregger Scott Kalafatas Timothy Faculty advisor: Professor Michael Moore #12;Greening Brownfield Properties A University of Michigan Brownfield Properties Abstract I In this project we provide a framework for analyzing the benefits

  13. Potato Green Bean Salad Ingredients

    E-Print Network [OSTI]

    Liskiewicz, Maciej

    Potato Green Bean Salad Ingredients: 6 potatoes 15 ounces green beans, canned 1 small red onion 3. Cover potatoes with cool water. Bring to boil over high heat. 3. Cover and reduce heat to medium, cook until potatoes are almost tender, about 15 minutes. 4. Meanwhile, open beans and pour into colander

  14. greenhouse gas inve green developmen

    E-Print Network [OSTI]

    Collins, Gary S.

    greenhouse gas inve green developmen energy conservation transportation carbon offs student facult;greenhouse gas inventory green development energy conservation transportation carbon offsets student faculty. Changi natural gas as a primary fuel allowed us to find cleaner and more effici university. Both in 1988

  15. Nuclear Resonance Fluorescence for Nuclear Materials Assay

    E-Print Network [OSTI]

    Quiter, Brian Joseph

    2010-01-01T23:59:59.000Z

    Potential of Nuclear Resonance Fluorescence . . . . . . . .2.9.1 Nuclear ThomsonSections . . . . . . . . . . . . . . . Nuclear Resonance

  16. Sandia National Laboratories: MOgene Green Chemicals LLC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MOgene Green Chemicals LLC Sandia to Partner with MOgene Green Chemicals on ARPA-E REMOTE Project On October 2, 2013, in Energy, News, News & Events, Partnership, Research &...

  17. Energy Department Issues Green Building Certification System...

    Office of Environmental Management (EM)

    Issues Green Building Certification System Final Rule to Support Increased Energy Measurement and Efficient Building Design Energy Department Issues Green Building Certification...

  18. Bioindustry Creates Green Jobs | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bioindustry Creates Green Jobs Bioindustry Creates Green Jobs Energy from abundant, renewable, domestic biomass can reduce U.S. dependence on oil, lower impacts on climate, and...

  19. The Green Cathedral: Sustainable Development of Amazonia

    E-Print Network [OSTI]

    Tobin, R. James

    1994-01-01T23:59:59.000Z

    The Green Cathedral: Sustainable Development of Amazonia R.The Green Cathedral: Sustainable Development of Amazonia. (Ais between sustainable and unsustainable development of the

  20. Green supercomputing at Argonne | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green supercomputing at Argonne Share Description Pete Beckman, head of Argonne's Leadership Computing Facility (ALCF) talks about Argonne National Laboratory's green...

  1. Greening up fossil fuels with carbon sequestration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Greening up fossil fuels with carbon sequestration 1663 Los Alamos science and technology magazine Latest Issue:May 2015 All Issues submit Greening up fossil fuels with carbon...

  2. City of Columbus- Green Columbus Fund

    Broader source: Energy.gov [DOE]

    The Green Columbus Fund incentivizes sustainable development and redevelopment in Columbus, Ohio. The Fund reimburses private and non-profit developers the application fee for the Green Building...

  3. Energy Innovation: Green Button Initiative Empowering Americans...

    Energy Savers [EERE]

    Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money Energy Innovation: Green Button Initiative Empowering Americans to Save Energy and Money...

  4. Fluorescence of molecular excimers

    SciTech Connect (OSTI)

    Nurmukhametov, R.N.; Sakhno, T.V.; Saukov, G.G.; Khakhel, O.A. [L. Ya. Karpov Research Physiochemical Institute, Moscow (Russian Federation)

    1995-11-01T23:59:59.000Z

    The phenomena of excimer fluorescence are most thoroughly studied in solutions of polycyclic aromatic hydrocarbons (PAHs). Therefore, excimer formation is usually treated with reference to molecules of this class of compounds. In the literature, only a qualitative picture of the energetics of excimer formation is given. It is assumed that dimerization of electron-excited and unexcited molecules is followed by the splitting of molecular electron-excited levels. PAH molecules are characterized by two lower excited levels: {sup 1}L{sub a} and {sup 1}L{sub b} (according to Platt`s classification). The S{sub 1}* state of some PAH compounds (e.g., naphthalene, phenanthrene, pyrene) is {sup 1}L{sub b}, while in other PAH (anthracene, naphthacene, perylene, etc.) it is the {sup 1}L{sub a} state. It is assumed that the {sup 1}L{sub a}-level is split more significantly than the {sup 1}L{sub b} level. Therefore, for all PAH investigated the excimer state is described as a lower-lying component of the split {sup 1}L{sub a} level. Quantum-chemical consideration of the splitting of electron levels of PAH molecules in excimers is undertaken. Unfortunately, in this case the description is also of a qualitative character. In the cited work, a correlation is noted between the energy of the {sup 1}L{sub a} state of the molecule and the wave number corresponding to the maximum of the emission band of the excimer. However, it does not give the wavelength of the maximum of the excimer band for some PAH, in other words, a definite dependence of the position of this band on molecular structure. The present work is devoted to a search for an answer to this question.

  5. GREEN ICT: How can ICT help to go Green Buildings?

    E-Print Network [OSTI]

    Khalil, E. E.; Kameel, R.

    2010-01-01T23:59:59.000Z

    , products, information, and allow us to cut traffic, but increase pollution and increase the negative ecological impact. Recently, an argumentative issue refers indirectly to the benefit of ICT to go green and decrease the negative ecological impact...

  6. Busting the Myth That Green Costs More Green 

    E-Print Network [OSTI]

    Qualk, J. D.; McCown, P.

    2008-01-01T23:59:59.000Z

    nearly 90 percent of their lives indoors, buildings are clearly important to our way of life. The most common misconception about green building is that these approaches cost more to implement than traditional strategies and techniques of design...

  7. Green GECCO | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluating AGeothermal/Exploration <GlacialGoldenarticle isin the NewsGreenGreenGreen

  8. AB 2160 GREEN BUILDING REPORT FOR SUBMISSION TO THE GOVERNOR'S

    E-Print Network [OSTI]

    CALIFORNIA ENERGY COMMISSION AB 2160 GREEN BUILDING REPORT FOR SUBMISSION TO THE GOVERNOR'S GREEN and Resource-Efficient (Green Building) Projects to the Governor's Green Action Team on several topics related to the 2004 Green Building Initiative

  9. Green roofs: potential at LANL

    SciTech Connect (OSTI)

    Pacheco, Elena M [Los Alamos National Laboratory

    2009-01-01T23:59:59.000Z

    Green roofs, roof systems that support vegetation, are rapidly becoming one of the most popular sustainable methods to combat urban environmental problems in North America. An extensive list of literature has been published in the past three decades recording the ecological benefits of green roofs; and now those benefits have been measured in enumerated data as a means to analyze the costs and returns of green roof technology. Most recently several studies have made substantial progress quantifying the monetary savings associated with storm water mitigation, the lessoning of the Urban Heat Island, and reduction of building cooling demands due to the implementation of green roof systems. Like any natural vegetation, a green roof is capable of absorbing the precipitation that falls on it. This capability has shown to significantly decrease the amount of storm water runoff produced by buildings as well as slow the rate at which runoff is dispensed. As a result of this reduction in volume and velocity, storm drains and sewage systems are relieved of any excess stress they might experience in a storm. For many municipalities and private building owners, any increase in storm water mitigation can result in major tax incentives and revenue that does not have to be spent on extra water treatments. Along with absorption of water, vegetation on green roofs is also capable of transpiration, the process by which moisture is evaporated into the air to cool ambient temperatures. This natural process aims to minimize the Urban Heat Island Effect, a phenomenon brought on by the dark and paved surfaces that increases air temperatures in urban cores. As the sun distributes solar radiation over a city's area, dark surfaces such as bitumen rooftops absorb solar rays and their heat. That heat is later released during the evening hours and the ambient temperatures do not cool as they normally would, creating an island of constant heat. Such excessively high temperatures induce heat strokes, heat exhaustion, and pollution that can agitate the respiratory system. The most significant savings associated with green roofs is in the reduction of cooling demands due to the green roof's thermal mass and their insulating properties. Unlike a conventional roof system, a green roof does not absorb solar radiation and transfer that heat into the interior of a building. Instead the vegetation acts as a shade barrier and stabilizes the roof temperature so that interior temperatures remain comfortable for the occupants. Consequently there is less of a demand for air conditioning, and thus less money spent on energy. At LANL the potential of green roof systems has already been realized with the construction of the accessible green roof on the Otowi building. To further explore the possibilities and prospective benefits of green roofs though, the initial capital costs must be invested. Three buildings, TA-03-1698, TA-03-0502, and TA-53-0031 have all been identified as sound candidates for a green roof retrofit project. It is recommended that LANL proceed with further analysis of these projects and implementation of the green roofs. Furthermore, it is recommended that an urban forestry program be initiated to provide supplemental support to the environmental goals of green roofs. The obstacles barring green roof construction are most often budgetary and structural concerns. Given proper resources, however, the engineers and design professionals at LANL would surely succeed in the proper implementation of green roof systems so as to optimize their ecological and monetary benefits for the entire organization.

  10. High throughput protein production screening

    DOE Patents [OSTI]

    Beernink, Peter T. (Walnut Creek, CA); Coleman, Matthew A. (Oakland, CA); Segelke, Brent W. (San Ramon, CA)

    2009-09-08T23:59:59.000Z

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  11. Green Data Centers: The European Sky

    E-Print Network [OSTI]

    Lefèvre, Laurent

    jobs. Energy Efficiency Plan 2011 Energy Efficiency Directive #12;1st Generation FIT4Green of resources (on/off) Cooperation between Data Centers GAMES: Green Active Management of Energy in IT Service)les Iteration #12;: GreenSDA & GreenSLA Active collaboration in data centre ecosystem to reduce energy

  12. BUILDING CODES: BARRIERS TO GREEN INNOVATION

    E-Print Network [OSTI]

    BUILDING CODES: BARRIERS TO GREEN INNOVATION JENNIFER GARMAN DR. JIM BOWYER DR. STEVE BRATKOVICH/18/2011 DOVETAIL PARTNERS, INC. www.dovetailinc.org Building Codes: Barriers to Green Innovation Introduction Many architects and contractors want to pursue green building design, technologies and construction. Green

  13. Green Impact Universities and Colleges: EXCELLENCE guidance

    E-Print Network [OSTI]

    Harman, Neal.A.

    Green Impact Universities and Colleges: EXCELLENCE guidance 1.0 Introduction Green Impact progressing through institutionally bespoke workbooks to green their departments and campuses, the need, rather than reaching a pre-set endpoint, so we are developing Green Impact to ensure our participants

  14. Lean Green Skating Machine Report No. 1

    E-Print Network [OSTI]

    Levi, Anthony F. J.

    Lean Green Skating Machine Report No. 1 Lean Green Skating Machine Report No. 1 Submitted by Simon Page 1 of 19 DISTRIBUTION STATEMENT: Distribution authorized to all. #12;Lean Green Skating Machine Report No. 1 Page 2 of 19 DISTRIBUTION STATEMENT: Distribution authorized to all. #12;Lean Green Skating

  15. Leafy Green Preparation and Cooling Study

    E-Print Network [OSTI]

    Leafy Green Preparation and Cooling Study Dominique N. Bibbins #12;Background : Leafy Greens z In the past, leafy greens have been the source of food borne-illnesses and outbreaks. z It is important Lasting knowledge of survey practices, procedures, and protocol. #12;Acknowledgements z Laura Green, Ph

  16. Building Environmental Sustainability Bowling Green State University

    E-Print Network [OSTI]

    Moore, Paul A.

    Building Environmental Sustainability at Bowling Green State University Final Report Bud Beatty September 2002 #12;ii Environmental Sustainability Committee Recommendations and Action Steps Bowling Green centrality to the mission of Bowling Green State University Action step needed: The "Bowling Green State

  17. Greek Green Beans and Tomato Ingredients

    E-Print Network [OSTI]

    Liskiewicz, Maciej

    garlic cloves 2 tomatoes 1 green pepper 2 cups water Directions 1. Wash the beans, cut off the tipsGreek Green Beans and Tomato Ingredients: 2 lbs green beans, fresh 2 teaspoons olive oil 2 onions 2. Cook until tender. 4. When onion is tender, add green beans and allow to cook, stir occasionally. 5

  18. Todd J. Greene Associate Professor

    E-Print Network [OSTI]

    Greene, Todd J.

    international area. Regional Studies of Rocky Mountains: Evaluated petroleum systems of Rocky Mountain basins for coalbed methane projects throughout Rocky Mountains. · Montgomery Community College Geology DepartmentTodd J. Greene Associate Professor Department of Geological and Environmental Sciences California

  19. Sandia National Laboratories: green transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    an easy task for start-up companies. But for clients of the i-GATE (Innovation for Green Advanced Transportation Excellence) innovation hub, there is a mechanism in place to...

  20. Broward County- Green Building Policy

    Broader source: Energy.gov [DOE]

    In October 2008, Board of County Commissioners of Broward County passed a resolution creating the County Green Building Policy. All new County-owned and operated buildings must achieve a minimum...

  1. Green Mountain Power- Solar GMP

    Broader source: Energy.gov [DOE]

    Green Mountain Power, an investor-owned electric utility operating in Vermont, offers a credit to customers with net-metered photovoltaic (PV) systems. In addition to the benefits of net metering,...

  2. Guide to Purchasing Green Power

    Broader source: Energy.gov [DOE]

    The Guide to Purchasing Green Power is intended for organizations that are considering the merits of buying green power as well as those that have decided to buy it and want help doing so. The guide was written for a broad audience, including businesses, government agencies, universities, and all organizations wanting to diversify their energy supply and reduce the environmental impact of their electricity use. The Guide can help with planning an on-site renewable generation project.

  3. The Greening of Government: A Study of How Governments Define the Green Agenda

    E-Print Network [OSTI]

    The Greening of Government: A Study of How Governments Define the Green Agenda Executive Summary Institute for Electronic Government, IBM Corporation Page 2 This page is intentionally blank. In the green Corporation Page 3 Foreword What are governments doing to `green' themselves? How do they define their green

  4. Green Fund Proposal Guidelines March 15, 2010 UNBC GREEN FUND PROPOSAL GUIDELINES

    E-Print Network [OSTI]

    Northern British Columbia, University of

    Green Fund Proposal Guidelines March 15, 2010 UNBC GREEN FUND PROPOSAL GUIDELINES In order to advance the overall sustainability of UNBC as an institution a "UNBC Green Fund" has been created from UNBC parking revenues. Green research and project proposals will be accepted by the Green University

  5. Dying Green A Film Screening and Panel Discussion about Green Burial

    E-Print Network [OSTI]

    Virginia Tech

    Dying Green A Film Screening and Panel Discussion about Green Burial March 20, 2014 6:00pm ­ 8:00pm to rest. The "Green Burial" movement is catching on in the U.S., and green cemetery options are now and panel discussion of the award-winning documentary, Dying Green (2011). Panel participants include Joshua

  6. Green Fund Proposal Guidelines September 15, 2010 UNBC GREEN FUND PROPOSAL GUIDELINES

    E-Print Network [OSTI]

    Northern British Columbia, University of

    Green Fund Proposal Guidelines September 15, 2010 UNBC GREEN FUND PROPOSAL GUIDELINES In order to advance the overall sustainability of UNBC as an institution a "UNBC Green Fund" has been created from UNBC parking revenues. Green research and project proposals will be accepted by the Green University

  7. Green Toys Inc.: Striving to Make Sustainable Products | 1 Green Toys Inc.

    E-Print Network [OSTI]

    Massachusetts at Lowell, University of

    Green Toys Inc.: Striving to Make Sustainable Products | 1 Green Toys Inc. Striving to Make Goeben of Green Toys Inc. for providing information for this case study and giving generously of his time of this report. 3 Background 4 The Genesis of Green Toys Inc. 5 Exploring What Makes Green Toys Greener

  8. GREEN UNIVERSITY COMMITTEE UNBC Green Strategy, Phase 1 (2009-2011)

    E-Print Network [OSTI]

    Northern British Columbia, University of

    - 1 - GREEN UNIVERSITY COMMITTEE UNBC Green Strategy, Phase 1 (2009-2011) 26 March 2009 PURPOSE The vision, objectives, and strategies contained in the UNBC Green Strategy, Phase 1 (2009- 2011 of the UNBC Green Strategy, Phase 1 (2009-2011) was based primarily on "UNBC's Green Strategy Discussion Paper

  9. Motivation Green's functions The GW Approximation The Bethe-Salpeter Equation Introduction to Green's functions

    E-Print Network [OSTI]

    Botti, Silvana

    Motivation Green's functions The GW Approximation The Bethe-Salpeter Equation Introduction to Green=whiteMotivation Green's functions The GW Approximation The Bethe-Salpeter Equation Outline 1 Motivation 2 Green's functions 3 The GW Approximation 4 The Bethe-Salpeter Equation #12;bg=whiteMotivation Green's functions

  10. Green Race, for Sustainability "Green Race for Sustainability" is environmental riding event which is funded by

    E-Print Network [OSTI]

    Haviland, David

    Green Race, for Sustainability "Green Race for Sustainability" is environmental riding event which and visit each city in central Sweden in August 2014. Apply via Green Race website http & We Need Your Helps. Let People See A Green Future From a long term vision, "Green Race

  11. Green Fund Proposal Guidelines August 10, 2012 UNBC GREEN FUND PROPOSAL GUIDELINES

    E-Print Network [OSTI]

    Northern British Columbia, University of

    Green Fund Proposal Guidelines August 10, 2012 UNBC GREEN FUND PROPOSAL GUIDELINES In order to advance the overall sustainability of UNBC as an institution a "UNBC Green Fund" has been created from UNBC parking revenues. Green research and project proposals are accepted by the Green University

  12. This article was downloaded by:[Bowling Green State University] [Bowling Green State University

    E-Print Network [OSTI]

    McGovern, Warren W.

    This article was downloaded by:[Bowling Green State University] [Bowling Green State University] On. © Taylor and Francis 2007 #12;DownloadedBy:[BowlingGreenStateUniversity]At:00:0621June2007 Communications and Statistics, Bowling Green State University, Bowling Green, Ohio, USA SP-domains were first introduced

  13. Leaky-mode assisted fluorescence extraction: application to fluorescence enhancement

    E-Print Network [OSTI]

    Cunningham, Brian

    to vertical radiation using a two-dimensional photonic crystal in a semiconductor light-emitting diode," Appl light-emitting diode," IEEE J. Quantum Electron. 36, 1131-1144 (2000). 3. S. Pillai, K. R. Catchpole, T. Trupke, G. Zhang, J. Zhao, and M. A. Green, "Enhanced emission from Si- based light-emitting diodes using

  14. Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

    E-Print Network [OSTI]

    Drennan, Catherine L.

    Chemical fluorophores offer tremendous size and photophysical advantages over fluorescent proteins but are much more challenging to target to specific cellular proteins. Here, we used Rosetta-based computation to design a ...

  15. NYSERDA's Green Jobs-Green New York Program: Extending Energy Efficiency Financing To Underserved Households

    E-Print Network [OSTI]

    Zimring, Mark

    2011-01-01T23:59:59.000Z

    Ho u s e h o ld s The New York legislature passed the GreenJobs-Green New York (GJGNY) Act in 2009. Administered by theand training for various ‘green- collar’ careers. Launched

  16. Covered Product Category: Fluorescent Ballasts

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and federal efficiency requirements across a variety of product categories, including fluorescent ballasts, which is a FEMP designated product category. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  17. Covered Product Category: Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  18. Fluorescent Magnetic Nanoparticles for Biomedical Applications

    E-Print Network [OSTI]

    Candea, George

    Fluorescent Magnetic Nanoparticles for Biomedical Applications V.M.Dao, Dr. G. Coullerez, Dr. L, the main goal was to synthesize and to characterize novel fluorescent magnetic nanoparticles. These nanoparticles (NPs) involve superparamagnetic iron oxide nanoparticles (SPIONs), a fluorescently-labeled polymer

  19. A cryogenic fluorescence spectroscopic study of uranyl carbonate...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    fluorescence spectroscopic study of uranyl carbonate, phosphate, and oxyhydroxide minerals. A cryogenic fluorescence spectroscopic study of uranyl carbonate, phosphate, and...

  20. Analytical approaches to photobiological hydrogen production in unicellular green algae

    E-Print Network [OSTI]

    Hemschemeier, Anja; Melis, Anastasios; Happe, Thomas

    2009-01-01T23:59:59.000Z

    E, Melis A (2000) Microalgae: a green source of renewable Hmany species of green microalgae. Moreover, it has attractedutili- zation of green microalgae and the process of

  1. SEP Success Story: "Green Launching Pad" Supports Clean Energy...

    Energy Savers [EERE]

    "Green Launching Pad" Supports Clean Energy Small Businesses SEP Success Story: "Green Launching Pad" Supports Clean Energy Small Businesses May 24, 2012 - 5:10pm Addthis Green...

  2. Attachment 1: Green Lease Policies and Procedures for Lease Acquisitio...

    Office of Environmental Management (EM)

    1: Green Lease Policies and Procedures for Lease Acquisition Attachment 1: Green Lease Policies and Procedures for Lease Acquisition Green Lease Policies and Procedures for Lease...

  3. Doing Well By Doing Good? Green Office Buildings

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2009-01-01T23:59:59.000Z

    sustainability, energy efficiency, green labels, real estateestate, the evidence on energy savings in green buildings isin Energy and Environmental Design”) green building rating

  4. Green's function analysis of periodic structures in computational electromagnetics

    E-Print Network [OSTI]

    Van Orden, Derek

    2011-01-01T23:59:59.000Z

    the two-dimensional periodic Green's function," IEEE Trans.evaluation of the Green's function for the Helmholtzrepresentations of certain Green's functions," J. Comp.

  5. The Green Library Movement: An Overview and Beyond

    E-Print Network [OSTI]

    Antonelli, Monika

    2008-01-01T23:59:59.000Z

    collaborate/index.php/Green_Librarianship_Sig_aboutus MetroCollaborate. ( 2008b, April 22). Green Librarianship.www.metro.org/collaborate.php/Green_Librarianship. Neale,

  6. Observation of green emission from Ce3+ doped gadolinium oxide...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    green emission from Ce3+ doped gadolinium oxide nanoparticles. Observation of green emission from Ce3+ doped gadolinium oxide nanoparticles. Abstract: Green emission at around 500...

  7. Advanced Interactive Facades - Critical Elements for Future Green Buildings?

    E-Print Network [OSTI]

    Selkowitz, Stephen; Aschehoug, Oyvind; Lee, Eleanor S.

    2003-01-01T23:59:59.000Z

    Elements for Future Green Buildings? Stephen Selkowitzelement for a “green building” that provides daylighting andcurrent interest in green buildings there was no shortage of

  8. The Green Library Movement: An Overview and Beyond

    E-Print Network [OSTI]

    Antonelli, Monika

    2008-01-01T23:59:59.000Z

    Board. (2008, January 15). Green Building Basics. RetrievedNewspapers database. U.S. Green Building Council. (n.d. )About the LEED Green Building Certification System.

  9. Doing Well By Doing Good? Green Office Buildings

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2009-01-01T23:59:59.000Z

    and Financial Benefits of Green Buildings , October 2003.average quality of the green buildings is somewhat higherquality of the non-green buildings in the clustered samples.

  10. Analytical approaches to photobiological hydrogen production in unicellular green algae

    E-Print Network [OSTI]

    Hemschemeier, Anja; Melis, Anastasios; Happe, Thomas

    2009-01-01T23:59:59.000Z

    monas reinhardtii (green alga). Planta 214:552–561. doi:adaptation in the green alga Chlamydomonas reinhardtii. Eurhydrogenase from the green alga Chlamydomonas reinhardtii.

  11. Review: Greening Citizenship: Sustainable Development, the State and Ideology

    E-Print Network [OSTI]

    Laberge, Yves

    2014-01-01T23:59:59.000Z

    Greening Citizenship: Sustainable Development, the State andGreening Citizenship: Sustainable Development, the State andGreening Citizenship: Sustainable Development, the State and

  12. Top 20 Ways to Go Green at Work (and Save the University Money!) Office Depot's three environmental aspirations are to "Buy Green," "Be Green" and

    E-Print Network [OSTI]

    Borenstein, Elhanan

    Top 20 Ways to Go Green at Work (and Save the University Money!) Office Depot's three environmental aspirations are to "Buy Green," "Be Green" and "Sell Green." · By "Buying Green," we have achieved the widest "green product assortment" in the office products industry - approximately 3,500 products with recycled

  13. Blue-green upconversion laser

    DOE Patents [OSTI]

    Nguyen, Dinh C. (Los Alamos, NM); Faulkner, George E. (Los Alamos, NM)

    1990-01-01T23:59:59.000Z

    A blue-green laser (450-550 nm) uses a host crystal doped with Tm.sup.3+. The Tm.sup.+ is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP.

  14. Blue-green upconversion laser

    DOE Patents [OSTI]

    Nguyen, D.C.; Faulkner, G.E.

    1990-08-14T23:59:59.000Z

    A blue-green laser (450--550 nm) uses a host crystal doped with Tm[sup 3+]. The Tm[sup 3+] is excited through upconversion by a red pumping laser and an IR pumping laser to a state which transitions to a relatively lower energy level through emissions in the blue-green band, e.g., 450.20 nm at 75 K. The exciting laser may be tunable dye lasers or may be solid-state semiconductor laser, e.g., GaAlAs and InGaAlP. 3 figs.

  15. GreenGov Presidential Award

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.NewofGeothermal Heat AnnualGreen BusinessGreenGov Presidential

  16. Green Dragon | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluating AGeothermal/Exploration <GlacialGoldenarticle isin the News HomeGreenGreen

  17. When Clouds become Green: the Green Open Cloud Architecture

    E-Print Network [OSTI]

    Boyer, Edmond

    of a new original energy-efficient Cloud infrastructure called Green Open Cloud. Keywords. Energy with the support of energy-efficient frameworks dedicated to Cloud architectures. Virtualization is a key feature of the energy-aware Cloud infras- tructure that we propose. The conclusion and future works are reviewed

  18. Fluorescence based chemical sensors for corrosion detection

    SciTech Connect (OSTI)

    Johnson, R.E. [LeTourneau Univ., Longview, TX (United States). Dept. of Chemistry; Agarwala, V.S. [Naval Air Warfare Center Aircraft Div., Patuxent River, MD (United States)

    1997-12-01T23:59:59.000Z

    Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.

  19. Fluorescent optical liquid level sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D. (Albuquerque, NM)

    2001-01-01T23:59:59.000Z

    A liquid level sensor comprising a transparent waveguide containing fluorescent material that is excited by light of a first wavelength and emits at a second, longer wavelength. The upper end of the waveguide is connected to a light source at the first wavelength through a beveled portion of the waveguide such that the input light is totally internally reflected within the waveguide above an air/liquid interface in a tank but is transmitted into the liquid below this interface. Light is emitted from the fluorescent material only in those portions of the waveguide that are above the air/liquid interface, to be collected at the upper end of the waveguide by a detector that is sensitive only to the second wavelength. As the interface moves down in the tank, the signal strength from the detector will increase.

  20. Greening existing buildings with LEED-EB!

    E-Print Network [OSTI]

    Dirksen, Tyson H

    2008-01-01T23:59:59.000Z

    The market of existing office buildings is going green. While early adopters of green buildings were owner-occupiers, there is a current wave of nonowner-occupied office buildings seeking Leadership in Energy and Environmental ...

  1. Permeable Pavements, Green Roofs, and Cisterns

    E-Print Network [OSTI]

    Hunt, William F.

    Permeable Pavements, Green Roofs, and Cisterns Stormwater Treatment Practices for Low site planning and engineer- pavements, green roofs, and cisterns, are ing to reduce or prevent cooperating. #12;Permeable Pavements What are they? Permeable pavements provide alternatives to standard

  2. Georgia Green Loans Save and Sustain Program

    Broader source: Energy.gov [DOE]

    Georgia Green Loans, a non-profit microlending agency, offers funding to "green" businesses using funding from a Georgia Environmental Finance Authority (GEFA) grant. The GEFA grant is based on...

  3. NC GreenPower Production Incentive

    Broader source: Energy.gov [DOE]

    '''''Note: NC GreenPower issued an RFP in December 2013, seeking up to 20,000 MWh of renewable energy credits (RECs) through a purchase with either a one or two year term. Green power is defined...

  4. Arlington County- Green Building Incentive Program

    Broader source: Energy.gov [DOE]

    In October 1999, the County Board of Arlington adopted a Pilot Green Building Incentive Program using the standards established by the U. S. Green Building Council’s Leadership in Energy and...

  5. GOVERNANCE DOCUMENTS BOWLING GREEN STATE UNIVERSITY

    E-Print Network [OSTI]

    Moore, Paul A.

    GOVERNANCE DOCUMENTS BOWLING GREEN STATE UNIVERSITY BOWLING GREEN, OH 43403 Governance Document I...........................................................................................I THE UNIVERSITY COMMUNITY............................................................................II Membership........................................................................................II.G THE PRESIDENT OF THE UNIVERSITY.................................................................III Presidential

  6. Green Button Initiative Growing | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Initiative Growing Green Button Initiative Growing May 17, 2013 - 1:17pm Addthis The Green Button initiative, which is the common-sense idea that electricity customers should...

  7. Charlotte Green Supply Chain: Residential Retrofitting | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Charlotte Green Supply Chain: Residential Retrofitting Charlotte Green Supply Chain: Residential Retrofitting July 30, 2010 - 10:50am Addthis Joshua DeLung What does this mean for...

  8. Green Button App Ideas | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    App Ideas Green Button App Ideas March 4, 2012 - 10:16pm Addthis Green Button App Ideas Matthew Loveless Matthew Loveless Data Integration Specialist, Office of Public Affairs...

  9. Green Codes and Programs | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Green Codes and Programs Green Codes and Programs Blue version of the EERE PowerPoint template, for use with PowerPoint 2007. Transcript Presentation More Documents & Publications...

  10. King County- Green Building Initiative (Washington)

    Broader source: Energy.gov [DOE]

    The King County Green Building Initiative started in 2001, and was included in the King Code Code with the Green Building and Sustainable Development Ordinance in 2008. The ordinance requires that...

  11. UAF Green Department Certification UAF OFFICE OF SUSTAINABILITY

    E-Print Network [OSTI]

    Hartman, Chris

    /Documentation: Computers are set to energy saving modes. Description/Documentation: GREEN DEPARTMENT PLEDGE UAF Green Department Certification UAF OFFICE OF SUSTAINABILITY GREEN DEPARTMENT CERTIFICATION INSTRUCTIONS & FORMS PART I: GETTING STARTED & REQUIRED ACTIONS #12; UAF Green Department Certification

  12. The Economic Development Potential of the Green Sector

    E-Print Network [OSTI]

    Ong, Paul M.; Patraporn, Rita Varisa

    2006-01-01T23:59:59.000Z

    in Los Angeles’ Green Building Sector. ” M.A. clientenvironment.html U.S. Green Building Council, 2003. BuildingFor High-Performance Green Buildings, U.S. Green Building

  13. Occupant satisfaction with indoor environmental quality in green buildings

    E-Print Network [OSTI]

    Abbaszadeh, S.; Zagreus, Leah; Lehrer, D.; Huizenga, C

    2006-01-01T23:59:59.000Z

    non-green) LEED-rated / green buildings Office Layout Officebuildings, and LEED-rated/green buildings Figure 2 shows aMedian of LEED- rated/green buildings Percentile Rank

  14. State Policies to Encourage Green Building Principles

    Broader source: Energy.gov [DOE]

    state green building policies, Database of State Incentives for Renewables and Efficiency, energy efficient building codes, energy efficient products

  15. Spectacle in the New Green City

    E-Print Network [OSTI]

    Lavine, Ethan

    2012-01-01T23:59:59.000Z

    their ecological footprints (Beatley 1999). Spectacle in theVolume 25, 2012 References Beatley, Timothy. Green Urbanism:

  16. Green Enterprise Development Program Overview

    E-Print Network [OSTI]

    Ginzel, Matthew

    for determining Environmental Footprint. Problem: Customers are requiring that BE GREEN, along with their entire Purdue Research Foundation C opyright2009 Purdue R esearch Foundation #12;AIR COMPRESSORS Most compressors deliver 4-5 cfm per 1 hp at 100 psi Every 2 psig of pressure increases or decreases the power

  17. Easy Gardening.....Green Beans

    E-Print Network [OSTI]

    Cotner, Sam; Parsons, Jerry; Masabni, Joseph

    2009-04-21T23:59:59.000Z

    ? Henderson Bush ? Jackson Wonder Soilpreparation Before planting green beans, remove all weeds and trash from the planting area. Then till the soil 8 to 10 inches deep and rake it several times to break up the large clods. It is best to work...

  18. June, 2008 From Green Business

    E-Print Network [OSTI]

    Hoffman, Andrew J.

    June, 2008 From Green Business Wondering where to start with your climate change strategy a company build a climate change strategy. The authors have very credible backgrounds for the task at hand? Climate Change: What's Your Business Strategy? offers a few useful tips. By Robert Colman It's nearly

  19. Green Jobs and Energy Economy

    E-Print Network [OSTI]

    Kammen, Daniel M.

    , that is The clean energy industry has been targeted as a key area for investment for three primary reasons: greaterGreen Jobs and the Clean Energy Economy ThoughT Leadership series Co-authors Daniel M. Kammen, Founding Director, Renewable and Appropriate Energy Laboratory University of California, Berkeley Ditlev

  20. Green Jobs and Energy Economy

    E-Print Network [OSTI]

    Kammen, Daniel M.

    as a key area for investment for three primary reasons: greater energy in- dependence, improvedGreen Jobs and the Clean Energy Economy THOUGHT LEADERSHIP SERIES Co-authors Ditlev Engel, Chief Distinguished Professor of Energy Founding Director, Renewable and Appropriate Energy Laboratory Co

  1. Cycling and Transit Green Buildings

    E-Print Network [OSTI]

    Lotze, Heike K.

    solar thermal panels at the LSC. 2. Solar wall on the Mona Campbell Bld. 3. 80 solar Photovoltaic (PV) panels installed in front of a solar wall system on the roof of the Computer Science Bld. Water Green Buildings Renewable Energy 1. Solar Thermal and PV Panels are mounted on C Building for research

  2. A Green Laser Pointer Hazard

    E-Print Network [OSTI]

    Jemellie Galang; Allesandro Restelli; Edward W. Hagley; Charles W. Clark

    2010-08-09T23:59:59.000Z

    An inexpensive green laser pointer was found to emit 20 mW of infrared radiation during normal use. This is potentially a serious hazard that would not be noticed by most users of such pointers. We find that this infrared emission derives from the design of the pointer, and describe a simple method of testing for infrared emissions using common household items.

  3. Green Systems Solar Hot Water

    E-Print Network [OSTI]

    Schladow, S. Geoffrey

    Green Systems Solar Hot Water Heating the Building Co-generation: Heat Recovery System: Solar panels not enough Generates heat energy Captures heat from generator and transfers it to water Stores Thermal Panels (Trex enclosure) Hot Water Storage Tank (TS-5; basement) Hot Water Heaters (HW-1

  4. Douglas H. Green Piper & Marbury

    E-Print Network [OSTI]

    such wastes are also subject to regulation under the Atomic Energy Act by the Nuclear Regulatory Commission type, of an exemption for commercial mixed waste is appropriate. In light of this rulemaking effort Signed: April 7, 1997] Re: Reproposal of HWIR Waste Rule and Commercial Mixed Waste Dear Mr. Green

  5. GreenHadoop: Leveraging Green Energy in Data-Processing Frameworks

    E-Print Network [OSTI]

    GreenHadoop: Leveraging Green Energy in Data-Processing Frameworks ´I~nigo Goiri Dept. of Computer that energy demand and supply must be matched, if we are to take full advantage of the green en- ergy workload to match the green energy supply. In particular, we consider data-processing frameworks, in which

  6. POMERIGGIO -GREEN WORKSHOP Ore 17:00 La riqualificazione edilizia in chiave green.

    E-Print Network [OSTI]

    Di Pillo, Gianni

    POMERIGGIO - GREEN WORKSHOP Ore 17:00 La riqualificazione edilizia in chiave green. Rifiuti urbani - SPETTACOLO Ore 22:00 Proiezione del docufilm "Green Generation", prodotto da Maiora Film in collaborazione con Rai Cinema. POMERIGGIO - GREEN WORKSHOP Ore 17:00 Mobilità sostenibile e Smart City. Soluzioni tra

  7. Green Streets Initiative Urban greening projects in Manchester, Trafford and Salford

    E-Print Network [OSTI]

    Green Streets Initiative Urban greening projects in Manchester, Trafford and Salford objectives Green Streets has captured the imagination of communities throughout Manchester, Trafford and Salford. The projects have not only improved their environment by planting trees, or greening walls and gable ends

  8. Green Clean Day Planning Guide A practical guide for creating a successful Green Clean Day

    E-Print Network [OSTI]

    Awtar, Shorya

    Green Clean Day Planning Guide A practical guide for creating a successful Green Clean Day A publication of U of M Waste Management Services July 2013 #12;Table of Contents What is a Green Clean Day? 2 Why have a Green Clean Day? 2 How Do We Get Started? 2 How Waste Management Services Can Help 2

  9. Track 2 _ A Green Building for Green Research The Problem NIATT Sustainable Transit Research Facility

    E-Print Network [OSTI]

    Kyte, Michael

    1 Track 2 _ A Green Building for Green Research The Problem NIATT Sustainable Transit Research President Tim White's Commitment to a Green Building The Concerns On-site water retention and treatment Building for Green Research Let's Make This Happen #12;

  10. Bowling Green State University * Bowling Green, OH 43403 School of HMSLS * 419-372-7234

    E-Print Network [OSTI]

    Moore, Paul A.

    Bowling Green State University * Bowling Green, OH 43403 School of HMSLS * 419-372-7234 Sport Management Bowling Green State University School of Human Movement, Sport, and Leisure Studies * 419-372-7234 * Like us on Facebook: Bowling Green State University Sport Management Follow us on Twitter: BGSUSport

  11. Green Computing input for better outcomes

    E-Print Network [OSTI]

    Amir, Yair

    Journal Profile: Udi Dahan Green Maturity Model for Virtualization Profiling Energy Usage for Efficient suggests that tracking energy consumption at every level will become the factor of success for greenGreen Computing input for better outcomes Learn the discipline, pursue the art, and contribute

  12. Green Sturgeon (Acipenser medirostris) Status Review Update

    E-Print Network [OSTI]

    Green Sturgeon (Acipenser medirostris) Status Review Update Biological Review Team Santa Cruz Status of Green Sturgeon 1 3. Key Questions in ESA Determination 3 a. The "Species" Question 3 b. The Extinction Risk Question 4 c. "A significant portion of the species' range" issue 4 Recently Updated Green

  13. Green Virtual Enterprises and their Breeding Environments

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Green Virtual Enterprises and their Breeding Environments David Romero, Arturo Molina Tecnológico. This paper introduces a Green Virtual Enterprise (GVE) model as an emerging sustainable manufacturing and logistics mode focused on offering, delivering and recovering green products to/from the market, under

  14. GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS

    E-Print Network [OSTI]

    Shoj, Toshiaki

    GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS TOSHIAKI SHOJI Department of Mathematics Science University of Tokyo Noda, Chiba 278­8510, Japan Abstract. Green functions of classical groups this, we define Green functions associated to complex reflection groups G(e, 1, n), and study

  15. CHANCELLOR'S SUSTAINABILITY COMMITTEE GREEN BUILDING SUBCOMMITTEE

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    CHANCELLOR'S SUSTAINABILITY COMMITTEE GREEN BUILDING SUBCOMMITTEE UNIVERSITY OF MASSACHUSETTS, policies and practices as they pertain to green building and LEED certification. It is our hope these guidelines as a framework for green building, we hope that design teams will be able to challenge

  16. Skills and Occupational Needs in Green Building

    E-Print Network [OSTI]

    European Union Skills and Occupational Needs in Green Building 2011 Jobs Jobs Transition Just Department #12;Skills and Occupational Needs in Green Building 2011 #12;#12;InternatIonal labour offIce · Geneva european commIssIon Skills and Occupational Needs in Green Building 2011 #12;photocomposed

  17. Trends in Utility Green Pricing Programs (2005)

    SciTech Connect (OSTI)

    Bird, L.; Brown, E.

    2006-10-01T23:59:59.000Z

    This report presents year-end 2005 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs.

  18. 24 January 2014 Green Impact Environmental Statement

    E-Print Network [OSTI]

    Banaji,. Murad

    24 January 2014 Green Impact Environmental Statement Human Resources Human Resources is one of the University of Kent's professional services departments involved in Green Impact. We gained Bronze Awards with an Excellence Project which will engage other Registry colleagues. Green Impact offers schools and departments

  19. Unusual expression of red fluorescence at M phase induced by anti-microtubule agents in HeLa cells expressing the fluorescent ubiquitination-based cell cycle indicator (Fucci)

    SciTech Connect (OSTI)

    Honda-Uezono, Asumi [Section of Oral Radiation Oncology, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan) [Section of Oral Radiation Oncology, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan); Section of Maxillofacial Surgery, Department of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan); Kaida, Atsushi [Section of Oral Radiation Oncology, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan)] [Section of Oral Radiation Oncology, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan); Michi, Yasuyuki; Harada, Kiyoshi [Section of Maxillofacial Surgery, Department of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan)] [Section of Maxillofacial Surgery, Department of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan); Hayashi, Yoshiki; Hayashi, Yoshio [Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392 (Japan)] [Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392 (Japan); Miura, Masahiko, E-mail: masa.mdth@tmd.ac.jp [Section of Oral Radiation Oncology, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan)] [Section of Oral Radiation Oncology, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549 (Japan)

    2012-11-16T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Fucci visualizes cell cycle by green and red fluorescence. Black-Right-Pointing-Pointer Plinabulin, induced unusual red fluorescence at M-phase in HeLa-Fucci cells. Black-Right-Pointing-Pointer The unusual pattern was followed by mitotic catastrophe. Black-Right-Pointing-Pointer The unusual pattern may be an early indicator of cell death in HeLa cells. -- Abstract: Plinabulin (NPI-2358) is a novel microtubule-depolymerizing agent. In HeLa cells, plinabulin arrests the cell-cycle at M phase and subsequently induces mitotic catastrophe. To better understand the effects on this compound on the cell-cycle, we used the fluorescent ubiquitination-based cell cycle indicator (Fucci), which normally enables G1 and S/G2/M cells to emit red and green fluorescence, respectively. When HeLa-Fucci cells were treated with 50 nM plinabulin, cells began to fluoresce both green and red in an unusual pattern; most cells exhibited the new pattern after 24 h of treatment. X-irradiation efficiently induced G2 arrest in plinabulin-treated cells and significantly retarded the emergence of the unusual pattern, suggesting that entering M phase is essential for induction of the pattern. By simultaneously visualizing chromosomes with GFP-histone H2B, we established that the pattern emerges after nuclear envelope breakdown but before metaphase. Pedigree assay revealed a significant relationship between the unusual expression and mitotic catastrophe. Nocodazole, KPU-133 (a more potent derivative of plinabulin), and paclitaxel also exerted similar effects. From these data, we conclude that the unusual pattern may be associated with dysregulation of late M phase-specific E3 ligase activity and mitotic catastrophe following treatment with anti-microtubule agents.

  20. Fluorescent Nanoparticles for Radiation DetectionFluorescent Nanoparticles

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.New MexicoFinancingProofWorking OutsideFluorescent Lightingfor

  1. The Green Computing Observatory: status of acquisition and analysis

    E-Print Network [OSTI]

    Lefèvre, Laurent

    The Green Computing Observatory: status of acquisition and analysis Cécile Germain-Renaud1, Julien, CNRS, INRIA 2: Laboratoire de l'Accélérateur Linéaire, CNRS-IN2P3 #12; Previous GreenDays talks o GreenDays@Paris The Green Computing Observatory: plans and scientific challenges o GreenDays@Lyon The Green Computing

  2. Using Green Roofs to Minimize Roof Runoff Pollution

    E-Print Network [OSTI]

    Clark, Shirley E.

    10/31/2008 1 Using Green Roofs to Minimize Roof Runoff Pollution Brett LongBrett Long Shirley Clark Courtesy Penn State Center for Green Roof Research Green Roofs:Green Roofs: The New BMP (at least in the US)The New BMP (at least in the US) Green roofs = vegetated roof coversGreen roofs = vegetated roof covers Pl

  3. Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging

    E-Print Network [OSTI]

    White, Katharine Alice

    2012-01-01T23:59:59.000Z

    Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. ...

  4. Saccharide sensing molecules having enhanced fluorescent properties

    DOE Patents [OSTI]

    Satcher Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-06T23:59:59.000Z

    The present invention provides formulae for fluorescent compounds that have a number of properties which make them uniquely suited for use in sensors of analytes such as saccharides. The advantageous fluorescent properties include favorable excitation wavelengths, emission wavelengths, fluorescence lifetimes, and photostability. Additional advantageous properties include enhanced aqueous solubility, as well as temperature and pH sensitivity. The compound comprises an aryl or a substituted phenyl botonic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  5. Characterization of folding the four-helix bundle protein Rop by real-time NMR

    E-Print Network [OSTI]

    Mochrie, Simon

    -loop-helix monomers. Protein folding moni- tored by stopped-flow fluorescence or CD exhibits biphasic kinetics when: protein folding/real-time NMR Introduction Rop is an RNA-binding protein that is involved in the regu of the effect on protein folding of the length of the connecting loop between the helices, led us to suggest

  6. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, Daniel L. (Albuquerque, NM); Tangyunyong, Paiboon (Albuquerque, NM)

    1998-01-01T23:59:59.000Z

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC.

  7. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, D.L.; Tangyunyong, P.

    1998-01-06T23:59:59.000Z

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC. 1 fig.

  8. Laser-Stimulated Fluorescence in Paleontology

    E-Print Network [OSTI]

    Kaye, Thomas G.; Falk, Amanda Renee; Pittman, Michael; Sereno, Paul C.; Martin, Larry D.; Burnham, David A.; Gong, Enpu; Xu, Xing; Wang, Yinan

    2015-05-27T23:59:59.000Z

    . doi: 10.1371/journal.pone. 0009223 PMID: 20169153 8. Warren TS, Gleason S, Bostwick RC, Verbeek ER. Ultraviolet light and fluorescent minerals: under- standing, collecting and displaying fluorescent minerals. GemGuides Book. 1999. 9. Hibbs AR. Confocal... fake and genuine vertebrate fossils. Journal of Paleontological Techniques. 2008; 2:1–5. Laser-Stimulated Fluorescence in Paleontology PLOS ONE | DOI:10.1371/journal.pone.0125923 May 27, 2015 22 / 22 ...

  9. LED Replacements for Linear Fluorescent Lamps Webcast

    Broader source: Energy.gov [DOE]

    In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting...

  10. Portable spotter for fluorescent contaminants on surfaces

    DOE Patents [OSTI]

    Schuresko, Daniel D. (Oak Ridge, TN)

    1980-01-01T23:59:59.000Z

    A portable fluorescence-based spotter for polynuclear aromatic hydrocarbon contamination on personnel and work area surfaces under ambient lighting conditions is provided. This instrument employs beam modulation and phase sensitive detection for discriminating between fluorescence from organic materials from reflected background light and inorganic fluorescent material. The device uses excitation and emission filters to provide differentiation between classes of aromatic organic compounds. Certain inorganic fluorescent materials, including heavy metal compounds, may also be distinguished from the organic compounds, despite both having similar optical properties.

  11. Covered Product Category: Compact Fluorescent Lamps

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance across a variety of product categories, including compact fluorescent lamps (CFLs), which are an ENERGY STAR-qualified product category.

  12. NREL: Sustainable NREL -Green Purchasing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What's Possible for Renewable Energy: Grid Integration NRELCost of6DataEnergyPhotographsVideosGreen

  13. New maturity indicators based on spectral fluorescence of alginite and bitumen, Monterey Formation, California

    SciTech Connect (OSTI)

    Lee, Chungi; Kennicutt, M.C. II (Texas A and M Univ., College Station (United States)); Lo, H.B. (Exxon Production Research Co., Houston, TX (United States))

    1991-03-01T23:59:59.000Z

    Conventional assessment of maturation level in the Monterey has been problematic, since sporinite and vitrinite are rare or absent. Organic matter is largely alginite and amorphous material, and reliable vitrinite reflectance (R{sub o}%) and Thermal Alteration Index (TAI) are difficult to obtain. Large amounts of bitumen often imbedded in the highly fractured Monterey shales cause a suppression of T{sub max} and low values of S{sub 1}S{sub 1} + S{sub 2}. It is often difficult to determine whether bitumen is indigenous or migrated from other more mature strata. Spectral fluorescence measurements of alginite and bitumen have proved useful in assessing the maturity of the Monterey. A maturity scale based on red/green quotient (Q{sub v}) measured as the fluorescence of alginite B when excited by violet-light has been developed and applied to the Monterey. Alginite B is common in the Monterey, and accurate fluorescent measurements can be readily obtained given the highly fluorescent character of alginite B. A total scanning fluorescence technique was used to develop a maturity scale based on bitumen aromatic content and composition. The maturity parameter (R{sub 1}) developed in this study uses the intensity of fluorescence emitted at 360 nm ratioed to that at 320 nm when the solvent-dissolved bitumen is excited at the 270 nm. These parameters allow for the evaluation of the thermal maturity of algal organic matter and bitumen from the Monterey with R{sub o}% {lt} 1. Indigenous bitumen is also indicated by a comparison of maturity based on Q{sub v} (the solid phase) and bitumen maturity (the liquid phase) based on R{sub 1}.

  14. FAA Fluorescent Penetrant Laboratory Inspections

    SciTech Connect (OSTI)

    WINDES,CONNOR L.; MOORE,DAVID G.

    2000-08-02T23:59:59.000Z

    The Federal Aviation Administration Airworthiness Assurance NDI Validation Center currently assesses the capability of various non-destructive inspection (NDI) methods used for analyzing aircraft components. The focus of one such exercise is to evaluate the sensitivity of fluorescent liquid penetrant inspection. A baseline procedure using the water-washable fluorescent penetrant method defines a foundation for comparing the brightness of low cycle fatigue cracks in titanium test panels. The analysis of deviations in the baseline procedure will determine an acceptable range of operation for the steps in the inspection process. The data also gives insight into the depth of each crack and which step(s) of the inspection process most affect penetrant sensitivities. A set of six low cycle fatigue cracks produced in 6.35-mm thick Ti-6Al-4V specimens was used to conduct the experiments to produce sensitivity data. The results will document the consistency of the crack readings and compare previous experiments to find the best parameters for water-washable penetrant.

  15. On the advantages of using green light to study fluorescence yield changes in leaves

    E-Print Network [OSTI]

    , at variance with the commonly used set-ups, uses of a weakly absorbed light source (light-emitting diodes. Although the exciting light is weakly absorbed, the possibility to tune the intensity of the light emitting diodes allows one to reach photochemical rates ranging from 104 s- 1 to 0.25 s- 1 rendering

  16. The Development of a High-throughput Microdroplet Bioreactor Device for Microbial Studies

    E-Print Network [OSTI]

    Guzman, Adrian

    2012-10-19T23:59:59.000Z

    Current PCR Polymerase Chain Reaction MOS-FET Metal-Oxide-Semiconductor Field-Effect Transistor BSA Bovine Serum Albumin GFP Green Fluorescent Protein RFP Red Fluorescent Protein LED Light Emitting Diode PMT Photomultiplier Tubes...

  17. The Development of a High-throughput Microdroplet Bioreactor Device for Microbial Studies 

    E-Print Network [OSTI]

    Guzman, Adrian

    2012-10-19T23:59:59.000Z

    Current PCR Polymerase Chain Reaction MOS-FET Metal-Oxide-Semiconductor Field-Effect Transistor BSA Bovine Serum Albumin GFP Green Fluorescent Protein RFP Red Fluorescent Protein LED Light Emitting Diode PMT Photomultiplier Tubes...

  18. Idahoans Saving Green by Going Green | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't YourTransport(Fact Sheet),Energy Petroleum Technology VisionImproperIdahoans Saving Green by

  19. A novel fluorescence detection method for organosilanes

    SciTech Connect (OSTI)

    Yang, H.X.; Craw, M.T.; Depew, M.C.; Wan, J.K.S. (Queen's Univ., Kingston, Ontario (Canada))

    1989-02-01T23:59:59.000Z

    Quinones such as phenanthraquinone and anthraquinone react with organosilanes giving persistent, strongly fluorescent radicals. The reactions are photochemically initiated, rapid, and facile; detection of concentrations of silanes in the 10{sup {minus}7} M range are readily achieved. The staring materials do not fluorescence making the procedure simple, selective and specific.

  20. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

    SciTech Connect (OSTI)

    Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

    2013-04-01T23:59:59.000Z

    Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ? Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ? Tri-molecular complexes containing two scFvs and one molecule GK were formed. ? GK was detected using fluorescence lifetime-based FRET imaging. ? GK is monomeric and homogeneously distributed in several cancer cell lines. ? This technique may have many applications in live-cell imaging of endogenous proteins.

  1. Quantifying the Comprehensive Greenhouse Gas Co-Benefits of Green Buildings

    E-Print Network [OSTI]

    Mozingo, Louise; Arens, Ed

    2014-01-01T23:59:59.000Z

    2010.     California  Green  Building  Standards  Code,  Team].    2008.   Green  Building  Sector  Subgroup:  cellular  data.  Green  Building  Information   Gateway  (

  2. A Study on Potential Environmental Benefits of Green Driving Strategies with NGSIM Data

    E-Print Network [OSTI]

    Jin, Wen-Long; Yuan, Daji; Yang, Hao

    2010-01-01T23:59:59.000Z

    before green driving, solid lines for those after greenbefore green driving, solid lines for those after green

  3. Applications of Nuclear Resonance Fluorescence

    SciTech Connect (OSTI)

    Warren, Glen A.; Detwiler, Rebecca S.; Peplowski, Patrick N.

    2010-11-11T23:59:59.000Z

    Nuclear Resonance Fluorescence (NRF) has the potential of addressing a wide variety of applications, which require isotopic and/or elemental information about a sample. We have investigated a variety of non-proliferation applications that may be addressed by NRF. From these applications, we have selected two, measuring uranium enrichment in UF6 cylinders and material verification in dismantlement, to investigate in more detail. Analytical models have been developed to evaluate these applications, and test measurements have been conducted to validate those models. We found that it is unlikely with current technology to address the requirements for UF6 cylinder enrichment measurements. In contrast, NRF is a very promising approach for material verification for dismantlement.

  4. Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri

    SciTech Connect (OSTI)

    Prochnik, Simon E.; Umen, James; Nedelcu, Aurora; Hallmann, Armin; Miller, Stephen M.; Nishii, Ichiro; Ferris, Patrick; Kuo, Alan; Mitros, Therese; Fritz-Laylin, Lillian K.; Hellsten, Uffe; Chapman, Jarrod; Simakov, Oleg; Rensing, Stefan A.; Terry, Astrid; Pangilinan, Jasmyn; Kapitonov, Vladimir; Jurka, Jerzy; Salamov, Asaf; Shapiro, Harris; Schmutz, Jeremy; Grimwood, Jane; Lindquist, Erika; Lucas, Susan; Grigoriev, Igor V.; Schmitt, Rudiger; Kirk, David; Rokhsar, Daniel S.

    2010-07-01T23:59:59.000Z

    Analysis of the Volvox carteri genome reveals that this green alga's increased organismal complexity and multicellularity are associated with modifications in protein families shared with its unicellular ancestor, and not with large-scale innovations in protein coding capacity. The multicellular green alga Volvox carteri and its morphologically diverse close relatives (the volvocine algae) are uniquely suited for investigating the evolution of multicellularity and development. We sequenced the 138 Mb genome of V. carteri and compared its {approx}14,500 predicted proteins to those of its unicellular relative, Chlamydomonas reinhardtii. Despite fundamental differences in organismal complexity and life history, the two species have similar protein-coding potentials, and few species-specific protein-coding gene predictions. Interestingly, volvocine algal-specific proteins are enriched in Volvox, including those associated with an expanded and highly compartmentalized extracellular matrix. Our analysis shows that increases in organismal complexity can be associated with modifications of lineage-specific proteins rather than large-scale invention of protein-coding capacity.

  5. Green Energy Standards for Public Buildings

    Broader source: Energy.gov [DOE]

    In March 2012, West Virginia enacted the Green Buildings Act, which applies to all new construction of public buildings, buildings receiving state grant funds, and buildings receiving state...

  6. Green Manufacturing Initiative Annual Report 2010

    E-Print Network [OSTI]

    de Doncker, Elise

    Green Manufacturing Initiative Annual Report 2010 Dr. John Patten Dr. David Meade May 3, 2011 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Energy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Herman Miller Energy Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

  7. ITEP Course: Greening Tribal Operations and Facilities

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Institute for Tribal Environmental Professionals will be offering a new course, Greening Tribal Operations and Facilities in San Diego, California, December 9 -11, 2014, for employees of...

  8. Green Energy Property Tax Assessment (Tennessee) | Department...

    Broader source: Energy.gov (indexed) [DOE]

    the Treasury Tennessee offers a special ad valorem property tax assessment for certified green energy production facilities. Property that generates electricity from a certified...

  9. High Performance Builder Spotlight: Green Coast Enterprises ...

    Energy Savers [EERE]

    - New Orleans, Louisiana Building America Whole-House Solutions for New Homes: Green Coast Enterprises, New Orleans, Louisiana Building America Best Practices Series...

  10. DEMEC - Green Energy Fund | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Delaware Department of Natural Resources and Environmental Control '''''Note: The Green Energy Fund regulations are currently under revision to improve program function and...

  11. Better Enzymes for Biofuels and Green Chemistry

    E-Print Network [OSTI]

    Better Enzymes for Biofuels and Green Chemistry: Solving the Cofactor Imbalance Problem Imbalances for the production of biofuels or other valuable chemicals. Though several research groups have re

  12. Trends in Utility Green Pricing Programs (2005)

    Broader source: Energy.gov [DOE]

    This report presents year-end 2005 data on utility green pricing programs, and examines trends in consumer response and program implementation over time. The data in this report, which were obtained via a questionnaire distributed to utility green pricing program managers, can be used by utilities to benchmark the success of their green power programs. It is important to note that this report covers only a portion of voluntary markets for renewable energy. It does not cover green power sold by independent marketers except for cases in which the marketers work in conjunction with utilities or default electricity suppliers.

  13. CHEVROLET | ELECTRIC | GREEN | SPARK EV | TECHNOLOGY. INNOVATION...

    Open Energy Info (EERE)

    CHEVROLET | ELECTRIC | GREEN | SPARK EV | TECHNOLOGY. INNOVATION & SOLUTIONS | GREENER VEHICLES Home There are currently no posts in this category. Syndicate content...

  14. City of San Francisco- Green Building Code

    Broader source: Energy.gov [DOE]

    San Francisco adopted a mandatory green building code for new construction projects in September 2008, establishing strict guidelines for residential and commercial buildings according to the...

  15. Hull Wind: A Community Gets Green

    Energy Savers [EERE]

    Hull Wind A Community Gets Green Community Wind Power National Renewable Energy Laboratory September 18, 2012 Andrew Stern Executive Director Action for Clean Energy, Inc. www....

  16. Green Initiatives Keep Hanford Site Environmentally Responsible...

    Energy Savers [EERE]

    and increasing recycling on site. "We at MSA are very proud to spearhead the site's green initiatives," said Lori Fritz, MSA's vice president of energy & environmental...

  17. National Green Building Standard Analysis

    SciTech Connect (OSTI)

    NAHB Research Center, Upper Marlboro, Maryland

    2012-07-01T23:59:59.000Z

    DOE's Building America Program is a research and development program to improve the energy performance of new and existing homes. The ultimate goal of the Building America Program is to achieve examples of cost-effective, energy efficient solutions for all U.S. climate zones. Periodic maintenance of an ANSI standard by review of the entire document and action to revise or reaffirm it on a schedule not to exceed five years is required by ANSI. In compliance, a consensus group has once again been formed and the National Green Building Standard is currently being reviewed to comply with the periodic maintenance requirement of an ANSI standard.

  18. Numerical calculation of Green's functions

    E-Print Network [OSTI]

    Urrea-Beltran, Julian

    1975-01-01T23:59:59.000Z

    , for his assistance in the preparation of this thesis, and Dr. Francis J. Narcowich and Dr. Phillip J. Green for serving as members of my committee. I am also grateful to Dr. Norman W. Naugle for hia guidance in using the Hewlett Packard Computer... concerning the numerical results (V) are given. The computations were done on the IBM 360 of Texas A&M University and the graphs on the Hewlett Packard 9830A digital computer of the Mathematics Department. The citations on the following pages follow...

  19. Green Enterprises | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, search OpenEI ReferenceJump to: navigation,II WindAirplane JumpToolboxGreenSW7

  20. Green Solar | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, search OpenEI ReferenceJump to: navigation,II WindAirplaneGreenEnergy

  1. Green Leases | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directed off Energy.gov. Are you sure you want toworldPowerHome| Department ofFormsDepartment ofGrantsGreen

  2. Merchant Green | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof Energy 2,AUDIT REPORTEnergyFarmsPower CoLongxing WindMaomingMerchant Green Jump to:

  3. Green Fuel | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page onYouTube YouTube Note: Since the.pdf Flash2006-52.pdf0.pdfDepartmentCounselGlass CoatingEducationfrom TexasGreen Fuel

  4. Final Green Zia Award Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.New Mexico Feb.Information 9 DefaultEnergy FinalGreen Zia

  5. Cashton Greens | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty EditCalifornia:Power LPInformationCashton Greens Jump to: navigation, search Name

  6. Green Circle | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluating AGeothermal/Exploration <GlacialGoldenarticle isin the News HomeGreen

  7. Green Highland | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluating AGeothermal/Exploration <GlacialGoldenarticle isin theGrowth BestGreen

  8. Green Shakti | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluating AGeothermal/Exploration <GlacialGoldenarticle isinEnergy,Green Shakti Jump

  9. Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid Oxide Fuel Cell Cathode Materials. Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid Oxide Fuel Cell...

  10. Using Nuclear Resonance Fluorescence for Nondestructive Isotopic Analysis

    E-Print Network [OSTI]

    Ludewigt, Bernhard A.

    2010-01-01T23:59:59.000Z

    7, (1959) pp. 54. [12] B.J. Quiter, ``Nuclear ResonanceFluorescence for Nuclear Materials Assay,'' University ofclandestine material with nuclear resonance fluorescence,"

  11. Super-Resolution Fluorescence Nanoscopy Applied to Image Core...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Super-Resolution Fluorescence Nanoscopy Applied to Image Core-Shell Photoswitching Nanoparticles and their Self-Assemblies . Super-Resolution Fluorescence Nanoscopy Applied to...

  12. GREEN BUILDING TRENDS How Green Building Impacted the National Capital Region Between 2003 and 2009

    E-Print Network [OSTI]

    GREEN BUILDING TRENDS How Green Building Impacted the National Capital Region Between 2003 and 2009 ecofriend.org 2 OVERVIEW What is LEED®? The LEED green building certification program is rapidly emerging as the standard in sustainable building design, construction, and operations. Scale of LEED Development Through

  13. Index to the Digest of Green Reports and Studies Complete "Green"

    E-Print Network [OSTI]

    Peter Taglia, P.G. In Digest http://www.ecw.org/MidwestBiogasPo tential.pdf www.labormarketinfo.edd.ca.gov/conte ntpub/GreenDigest/Biogas-Rethinking- the-Midwest-Potential.pdf Building a Clean Energy WorkforceIndex to the Digest of Green Reports and Studies Complete "Green" Publication Title Author

  14. From: Methods in Molecular Biology, vol. 350: Protein Folding Protocols Edited by: Y. Bai and R. Nussinov Humana Press Inc., Totowa, NJ

    E-Print Network [OSTI]

    Schuler, Ben

    115 From: Methods in Molecular Biology, vol. 350: Protein Folding Protocols Edited by: Y. Bai and R to Protein Folding Benjamin Schuler Summary Protein folding is a process characterized by a large degree this method to protein folding. Key Words: Protein folding; fluorescence spectroscopy; single molecule

  15. Glucose sensing molecules having selected fluorescent properties

    DOE Patents [OSTI]

    Satcher, Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-27T23:59:59.000Z

    An analyte sensing fluorescent molecule that employs intramolecular electron transfer is designed to exhibit selected fluorescent properties in the presence of analytes such as saccharides. The selected fluorescent properties include excitation wavelength, emission wavelength, fluorescence lifetime, quantum yield, photostability, solubility, and temperature or pH sensitivity. The compound comprises an aryl or a substituted phenyl boronic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. The fluorophore and switch component are selected such that the value of the free energy for electron transfer is less than about 3.0 kcal mol.sup.-1. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  16. Hiding patterns with daylight fluorescent inks

    E-Print Network [OSTI]

    Romain Rossier; Roger D. Hersch; School Of Computer; Communication Sciences; Ecole Polytechnique; Fédérale Lausanne

    We propose a method for hiding patterns within printed images by making use of classical and of two daylight fluorescent magenta and yellow inks. Under the D65 illuminant we establish in the CIELAB space the gamut of a classical cmyk printer and the gamut of the same printer using a combination of classical inks with daylight fluorescent inks. These gamuts show that a significant part of the classical ink gamut can be reproduced by combining classical inks with daylight fluorescent inks. By printing parts of images with a combination of classical and daylight fluorescent inks instead of using classical inks only, we can hide security patterns within printed images. Under normal daylight, we do not see any difference between the parts printed with classical inks only and the parts printed with daylight fluorescent inks and classical inks. By changing the illumination, e.g. by viewing the printed image under a tungsten lamp or under a UV lamp, the daylight fluorescent inks change their colors and reveal the security pattern formed by combinations of classical inks and of daylight fluorescent inks.

  17. Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching

    E-Print Network [OSTI]

    Govindjee

    Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein a fluorescence were made on leaves of avocado plants to study whether rapidly reversible Dp

  18. Home Articles Directions Contact Green Resources & Links The Green Building Gallery Posted by Branden Klayko: Broken Sidewalk.

    E-Print Network [OSTI]

    Home Articles Directions Contact Green Resources & Links The Green Building Gallery Posted by Branden Klayko: Broken Sidewalk. The Green Building on East Market Street in the new ly named Nu The Green Building green. The 110-year-old structure is in the running for LEED Platinum certification

  19. Green Roof Media Selection forGreen Roof Media Selection for the Minimization of Pollutantthe Minimization of Pollutant

    E-Print Network [OSTI]

    Clark, Shirley E.

    1 Green Roof Media Selection forGreen Roof Media Selection for the Minimization of Pollutantthe Minimization of Pollutant Loadings in Roof RunoffLoadings in Roof Runoff Brett LongBrett Long Shirley Clark for Green Roof Research Green Roofs:Green Roofs: The New BMP (at least in the US)The New BMP (at least

  20. Doing Well by Doing Good? Green Office Buildings

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2009-01-01T23:59:59.000Z

    estimated for a green building, or to Energy Star. 2008. “square foot) Green rating (1 = yes) Energy Star (1 = yes)square foot) Green rating (1 = yes) Energy Star (1 = yes)

  1. GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS, II

    E-Print Network [OSTI]

    Shoj, Toshiaki

    GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS, II University of Tokyo Noda, Chiba 278-8510, Japan Abstract. Green functions and Hall-Littlewoo* *d functions associated to W are introduced, and Green functions are described

  2. Doing Well by Doing Good? Green Office Buildings

    E-Print Network [OSTI]

    Eichholtz, Piet; Kok, Nils; Quigley, John M.

    2009-01-01T23:59:59.000Z

    dummy variables, one for each green building in the sample.dummy variables, one for each green building in the sample.nificantly higher for green buildings than for the most “

  3. Sustainable Building in China - A Green Leap Forward?

    E-Print Network [OSTI]

    Diamond, Richard C.

    2014-01-01T23:59:59.000Z

    building compare to other green buildings in China and theKora, A. Re-Assessing Green Building Performance: A Postencouraged by the nascent green building movement that is

  4. Green Campus Committee 2009/2010 Buildings & Estates Office

    E-Print Network [OSTI]

    Schellekens, Michel P.

    Green Campus Committee 2009/2010 Buildings & Estates Office Barrie Curley Maurice Ahern, Stephan An Taisce Ellen Murphy, Green Schools Development Officer Birgit O'Driscoll, Green Schools Development

  5. aluminide green sheet: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Geaux Green: GREEN GUIDE A GUIDE TO LIVING SUSTAINABLY 12;2 Table of Contents Geauxing Green p. 1-30 Transportation 3 Water 4 Energy 5 Food 6 Recycling 7 Around Baton Rouge p. 8...

  6. adult green lacewings: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Geaux Green: GREEN GUIDE A GUIDE TO LIVING SUSTAINABLY 12;2 Table of Contents Geauxing Green p. 1-30 Transportation 3 Water 4 Energy 5 Food 6 Recycling 7 Around Baton Rouge p. 8...

  7. african green monkeys: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Geaux Green: GREEN GUIDE A GUIDE TO LIVING SUSTAINABLY 12;2 Table of Contents Geauxing Green p. 1-30 Transportation 3 Water 4 Energy 5 Food 6 Recycling 7 Around Baton Rouge p. 8...

  8. african green monkey: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Geaux Green: GREEN GUIDE A GUIDE TO LIVING SUSTAINABLY 12;2 Table of Contents Geauxing Green p. 1-30 Transportation 3 Water 4 Energy 5 Food 6 Recycling 7 Around Baton Rouge p. 8...

  9. Solving the 'Green Gap' in LED Technology

    Broader source: Energy.gov [DOE]

    One long-standing high-priority research area for DOE is to increase the efficiency of deep green LEDs. Although most products today use phosphor conversion (PC) to produce white light from a blue LED, having a good green source could lead to color-mixed white sources that would avoid the losses associated with the PC approach.

  10. SOLAR TODAY28 The Green Mountain Energysm

    E-Print Network [OSTI]

    , generates pollution-free, renewable electricity. GreenMountainEnergyCompany BuyingGreenPower-- You of renewable energy technologies. by Blair Swezey and Lori Bird #12;January/February 2003 29 The electricity that we use in the U.S. is primarily generated with coal (52 percent), nuclear (20 percent) and natural

  11. Green Homebuilding By Design- a Systems Approach

    E-Print Network [OSTI]

    Pfeiffer, P. L.

    expectations, climatically appropriate passive solar design, US energy independence and global warming. ? Clearly articulate the relative value of thoughtful programming and a systems approach to passive solar design. ? Prioritize and apply high... Seminar proposal: Green Homebuilding By Design ? a Systems Approach Subtitle: Connecting the dots between high performance design & appropriate ?vetted out? green building strategies Brief...

  12. Green's functions for Neumann boundary conditions

    E-Print Network [OSTI]

    Jerrold Franklin

    2012-08-27T23:59:59.000Z

    Green's functions for Neumann boundary conditions have been considered in Math Physics and Electromagnetism textbooks, but special constraints and other properties required for Neumann boundary conditions have generally not been noticed or treated correctly. In this paper, we derive an appropriate Neumann Green's function with these constraints and properties incorporated.

  13. University Town Management Office Town Green Usage

    E-Print Network [OSTI]

    Chaudhuri, Sanjay

    University Town Management Office Town Green Usage Background The UTown Management Office (UTMO the sustainability of the Green as the Office of Estate & Development (OED) has highlighted that the landscape consultant has advised that it would require about one year period for the soil to settle to its final level

  14. BOWLING GREEN STATE UNIVERSITY 16 September 2011

    E-Print Network [OSTI]

    Moore, Paul A.

    BOWLING GREEN STATE UNIVERSITY 16 September 2011 MEMORANDUM To: Rodney Rogers, Interim Senior Vice education at Bowling Green State University. It owes much to the thinking of the first group, but it has the current thinking of the second of two university groups whose task was to re-envision undergraduate

  15. Supported by GreenNet-EU27

    E-Print Network [OSTI]

    Figueiredo Integration costs of wind due to changed system operation and investment decisions in GermanySupported by GreenNet-EU27 EIE/04/049/S07.38561 GreenNet-EU27 GUIDING A LEAST COST GRID INTEGRATION cost and grid extension cost caused by intermittent RES-E grid integration Derk Jan Swider (Ed

  16. University ofWinnipeg Green Campus

    E-Print Network [OSTI]

    Martin, Jeff

    | sustainability.uwinnipeg.ca | Page 5 Although a device is turned off, it is using energy when it is pluggedUniversity ofWinnipeg Green Campus User's Guide by LenaYusim Campus Sustainability Office University of Winnipeg 515 Portage Avenue R3B 2E9 sustainability.uwinnipeg.ca #12;Green Campus User's Manual

  17. 1206 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim DOI: 10.1002/cphc.200400609 ChemPhysChem 2005, 6, 1206 1220 Single-Molecule Fluorescence Spectroscopy of

    E-Print Network [OSTI]

    Schuler, Ben

    , 6, 1206 ­ 1220 #12;Single-Molecule Fluorescence Spectroscopy of Protein Folding Benjamin Schuler prevented a comprehensive solution of the protein-folding problem. Nevertheless, our understand- ing of protein-folding mechanisms has increased dramatically, and single-molecule spectroscopy has started

  18. Suggested Courses for ME Students Interested in Green Engineering Please see http://www.eng.vt.edu/green/ for information on the Green Engineering minor.

    E-Print Network [OSTI]

    Virginia Tech

    for increasing efficiencies (energy storage, batteries, green building, conservation). Options for transportationSuggested Courses for ME Students Interested in Green Engineering Please see http://www.eng.vt.edu/green/ for information on the Green Engineering minor. Required Courses: ME 4015-4016 ­ Engineering Design and Project (6

  19. Why are most plants Green? By basic observation we know that live healthy plants have a green pigment. Grass is green, the

    E-Print Network [OSTI]

    Bertulani, Carlos A. - Department of Physics and Astronomy, Texas A&M University

    Why are most plants Green? By basic observation we know that live healthy plants have a green pigment. Grass is green, the leaves on the trees outside are green nearly every plant that utilizes plants interact with different wavelengths of light and what they mean. Figure 1: Multispectral image

  20. GREEN GALAXIES IN THE COSMOS FIELD

    SciTech Connect (OSTI)

    Pan, Zhizheng; Kong, Xu; Fan, Lulu, E-mail: panzz@mail.ustc.edu.cn, E-mail: xkong@ustc.edu.cn [Center of Astrophysics, University of Science and Technology of China, Hefei 230026 (China)

    2013-10-10T23:59:59.000Z

    We present research on the morphologies, spectra, and environments of ?2350 'green valley' galaxies at 0.2 < z < 1.0 in the COSMOS field. The bimodality of dust-corrected NUV–r {sup +} color is used to define 'green valley'; it removes dusty star-forming galaxies from galaxies that are truly transitioning between the blue cloud and the red sequence. Morphological parameters of green galaxies are intermediate between those of blue and red galaxy populations, both on the Gini-asymmetry and the Gini-M{sub 20} planes. Approximately 60%-70% of green disk galaxies have intermediate or big bulges, and only 5%-10% are pure disk systems, based on morphological classification using the Zurich Estimator of Structural Types. The obtained average spectra of green galaxies are intermediate between blue and red ones in terms of [O II], H?, and H? emission lines. Stellar population synthesis on the average spectra shows that green galaxies are on average older than blue galaxies but younger than red galaxies. Green galaxies and blue galaxies have similar projected galaxy density (?{sub 10}) distributions at z > 0.7. At z < 0.7, the fractions of M{sub *} < 10{sup 10.0} M{sub ?} green galaxies located in a dense environment are found to be significantly larger than those of blue galaxies. The morphological and spectral properties of green galaxies are consistent with the transitioning population between the blue cloud and the red sequence. The possible mechanisms for quenching star formation activities in green galaxies are discussed. The importance of active galactic nucleus feedback cannot be well constrained in our study. Finally, our findings suggest that environmental conditions, most likely starvation and harassment, significantly affect the transformation of M{sub *} < 10{sup 10.0} M{sub ?} blue galaxies into red galaxies, especially at z < 0.5.

  1. Fluorescence Imaging for Nuclear Arms Control Verification

    E-Print Network [OSTI]

    Feener, Jessica S

    2014-08-14T23:59:59.000Z

    treaties. Specifically, this technique uses fluorescence imaging to determine fissile material attributes in verifying, during the dismantlement process, an uncanned nuclear warhead or warhead component without revealing sensitive information. This could...

  2. Fluorescence-based optical glucose sensing 

    E-Print Network [OSTI]

    Meledeo, Michael Adam

    2002-01-01T23:59:59.000Z

    Previous studies have indicated that optical means of blood glucose detection are feasible and provide a minimally invasive alternative to current commercially available modalities. Fluorescence spectroscopy has shown promise in many examples...

  3. Using Nuclear Resonance Fluorescence for Nondestructive Isotopic Analysis

    E-Print Network [OSTI]

    Ludewigt, Bernhard A.

    2010-01-01T23:59:59.000Z

    spent fuel assay using Nuclear Resonance Fluorescence,” Annual Meeting of the Institute of Nuclear Materials Management,

  4. Expanded "Green Button" Will Reach Federal Agencies and More...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    "Green Button" Will Reach Federal Agencies and More American Energy Consumers Expanded "Green Button" Will Reach Federal Agencies and More American Energy Consumers December 6,...

  5. Attachment 2: Solicitation for Offers with New and Revised Green...

    Office of Environmental Management (EM)

    2: Solicitation for Offers with New and Revised Green Lease Text Attachment 2: Solicitation for Offers with New and Revised Green Lease Text Solicitation for Offers Paragraphs with...

  6. SEP Success Story: Launching Green Entrepreneurship in New Hampshire...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    NH. | Courtesy of University of New Hampshire Photographic Services SEP Success Story: "Green Launching Pad" Supports Clean Energy Small Businesses SEP Success Story: Green...

  7. Obama Administration Announces $12 Million i6 Green Investment...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    12 Million i6 Green Investment to Promote Clean Energy Innovation and Job Creation Obama Administration Announces 12 Million i6 Green Investment to Promote Clean Energy...

  8. City of Pittsburgh Implementation Model: Green Initiatives Trust...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Model: Green Initiatives Trust Fund City of Pittsburgh implementation model, Green initiatives trust fund. Author: U. S. Department of Energy City of Pittsburgh...

  9. Forest County Potawatomi Tribe Cuts Emissions, Promotes Green...

    Office of Environmental Management (EM)

    Emissions, Promotes Green Growth Forest County Potawatomi Tribe Cuts Emissions, Promotes Green Growth February 23, 2012 - 6:29pm Addthis The Forest County Potawatomi Tribe's solar...

  10. Guide to Purchasing Green Power: Renewable Electricity, Renewable...

    Energy Savers [EERE]

    Guide to Purchasing Green Power: Renewable Electricity, Renewable Energy Certificates, and On-Site Renewable Generation Guide to Purchasing Green Power: Renewable Electricity,...

  11. Obama Administration Announces Launch of i6 Green Challenge to...

    Office of Environmental Management (EM)

    Obama Administration Announces Launch of i6 Green Challenge to Promote Clean Energy Innovation and Economic Growth Obama Administration Announces Launch of i6 Green Challenge to...

  12. From Tragedy to Triumph - Rebuilding Green Buildings after Disaster...

    Office of Environmental Management (EM)

    Rebuilding Green Buildings after Disaster, EERE (Fact Sheet) From Tragedy to Triumph - Rebuilding Green Buildings after Disaster, EERE (Fact Sheet) Fact sheet offering practical...

  13. Attachment 3: Instructions for Use of Green Lease Solicitation...

    Office of Environmental Management (EM)

    3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart Attachment 3: Instructions for Use of Green Lease Solicitation Paragraph Reference Chart...

  14. EPA Green Power Leadership Awards | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    EDT U.S. Environmental Protection Agency The U.S. Environmental Protection Agency (EPA) Green Power Leadership Awards recognize exceptional achievement among EPA Green Power...

  15. EECBG Success Story: David Crockett, Chattanooga's Green Frontiersman...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    David Crockett, Chattanooga's Green Frontiersman EECBG Success Story: David Crockett, Chattanooga's Green Frontiersman June 11, 2010 - 5:15pm Addthis Crockett has seen Chattanooga...

  16. Beyond Laboratories, Beyond Being Green | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Beyond Laboratories, Beyond Being Green Beyond Laboratories, Beyond Being Green Presentation covers sustainable laboratories and the Labs21 program given at the Federal Utility...

  17. Los Angeles County's Green Idea House Achieves Efficient Goals...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    County's Green Idea House Achieves Efficient Goals Los Angeles County's Green Idea House Achieves Efficient Goals Photo of an energy-efficient home with modern architecture. The...

  18. Local Energy Alliance Program Adds Green Appraisal Capabilities...

    Energy Savers [EERE]

    Local Energy Alliance Program Adds Green Appraisal Capabilities to its Energy Efficiency Services Local Energy Alliance Program Adds Green Appraisal Capabilities to its Energy...

  19. Highly Energy Efficient Directed Green Liquor Utilization (D...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Highly Energy Efficient Directed Green Liquor Utilization (D-GLU) Pulping Highly Energy Efficient Directed Green Liquor Utilization (D-GLU) Pulping This factsheet describes a...

  20. NATIONAL RADIO ASTRONOMY OBSERVATORY GREEN BANK, WEST VIRGINIA

    E-Print Network [OSTI]

    Groppi, Christopher

    NATIONAL RADIO ASTRONOMY OBSERVATORY GREEN BANK, WEST VIRGINIA ELECTRONICS DIVISION TECHNICAL NOTE R. Lacasse #12;NATIONAL RADIO ASTRONOMY OBSERVATORY Green Bank, West Virginia 140-ft CASSEGRAIN BAa

  1. Korea's Green Growth Strategy: Mitigating Climate Change and...

    Open Energy Info (EERE)

    Korea's Green Growth Strategy: Mitigating Climate Change and Developing New Growth Engines Jump to: navigation, search Name Korea's Green Growth Strategy: Mitigating Climate Change...

  2. Promoting a Green Economy through Clean Transportation Alternatives...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    D.C. tiarravt052ebert2010p.pdf More Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean...

  3. Greening Federal Facilities: An Energy, Environmental, and Economic...

    Energy Savers [EERE]

    Greening Federal Facilities: An Energy, Environmental, and Economic Resource Guide for Federal Facility managers and Designers; Second Edition Greening Federal Facilities: An...

  4. ARM - Field Campaign - Observations and Modeling of the Green...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    govCampaignsObservations and Modeling of the Green Ocean Amazon: SKIP Pre-campaign Measurements Related Campaigns Observations and Modeling of the Green Ocean Amazon (GOAMAZON...

  5. ARM - Field Campaign - Observations and Modeling of the Green...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    govCampaignsObservations and Modeling of the Green Ocean Amazon: CCN Activity of Aerosols Related Campaigns Observations and Modeling of the Green Ocean Amazon (GOAMAZON 2014)...

  6. Green approach for self-assembly of platinum nanoparticles into...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Green approach for self-assembly of platinum nanoparticles into nanowires in aqueous glucose solutions. Green approach for self-assembly of platinum nanoparticles into nanowires in...

  7. Alternative Electrostatic Green's Function for a Long Tube. ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Alternative Electrostatic Green's Function for a Long Tube. Alternative Electrostatic Green's Function for a Long Tube. Abstract: This note describes an expression for the...

  8. The Energy Efficiency and Renewable Energy (EERE) blog on Green...

    Open Energy Info (EERE)

    The Energy Efficiency and Renewable Energy (EERE) blog on Green Button is live Home > Groups > Green Button Applications Dc's picture Submitted by Dc(266) Contributor 27 February,...

  9. City of Dallas- Green Building Expedited Plan Review

    Broader source: Energy.gov [DOE]

    The Dallas Green Building Program establishes expedited permitting for green buildings. During Phase 1 of the program (October 1, 2009 - September 30, 2011), applicants must provide a checklist...

  10. Executive Order 13148-Greening the Government Through Leadership...

    Energy Savers [EERE]

    148-Greening the Government Through Leadership in Environmental Management Executive Order 13148-Greening the Government Through Leadership in Environmental Management The head of...

  11. affect green roof: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Early green roof cooling and energy reduction research in North America took place in Canada and the northern latitudes of the United States, where green roofs reduced rooftop...

  12. Fuel Cell Technologies Researcher Lightens Green Fuel Production...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fuel Cell Technologies Researcher Lightens Green Fuel Production Fuel Cell Technologies Researcher Lightens Green Fuel Production August 25, 2014 - 9:36am Addthis Research funded...

  13. Greensburg, Kansas: Building a Model Green Community, How Would...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Greensburg, Kansas: Building a Model Green Community, How Would You Rebuild a Town - Green? April 2009 (Brochure) This brochure describes the rebuilding of Greensburg, Kansas,...

  14. Retooled Machines Bring New Green Jobs to Illinois | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Reinvestment Act. The Illinois Energy Plan office aims to invest and develop the state's green economy, including renewable energy sources, energy efficiency and green buildings....

  15. Energy Challenge Three: The Greenbelt Green Neighborhood Challenge...

    Broader source: Energy.gov (indexed) [DOE]

    Energy Challenge Three: The Greenbelt Green Neighborhood Challenge Energy Challenge Three: The Greenbelt Green Neighborhood Challenge June 21, 2010 - 5:52pm Addthis John Lippert...

  16. 3800 Green Series Cost Elements | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    (formerly EPP) Program 3800 Green Series Cost Elements 06112014 (Rev. 7) 3800 Green Series Cost Elements More Documents & Publications 1 OPAM Policy Acquisition Guides...

  17. EPA Webcast - Creating a Green Infrastructure Plan to Transform...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EPA Webcast - Creating a Green Infrastructure Plan to Transform Your Community EPA Webcast - Creating a Green Infrastructure Plan to Transform Your Community November 18, 2014...

  18. Promoting a Green Economy through Clean Transportation Alternatives...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Peer Evaluation arravt052tiebert2011p.pdf More Documents & Publications Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through...

  19. Greening Your Job: Careers in Energy | Department of Energy

    Energy Savers [EERE]

    energy and energy efficiency related careers. One of these fact sheets, entitled Green Energy Technologies Create Green Jobs (PDF 1.6 MB) , provides an interesting way to...

  20. Promoting a Green Economy through Clean Transportation Alternatives...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Promoting a Green Economy through Clean Transportation Alternatives Promoting a Green Economy through Clean Transportation Alternatives Town of Hempstead: Project Energy,...

  1. Webinar: Creating a Green Infrastructure Plan to Transform Your...

    Broader source: Energy.gov (indexed) [DOE]

    Webinar: Creating a Green Infrastructure Plan to Transform Your Community Webinar: Creating a Green Infrastructure Plan to Transform Your Community November 18, 2014 3:00PM to...

  2. Enhancing low-carbon development by greening the economy: policy...

    Open Energy Info (EERE)

    low-carbon development by greening the economy: policy dialogue, advisory services, benchmarking Jump to: navigation, search Name Enhancing low-carbon development by greening the...

  3. alizarine fast green: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    print advertising and trade show booths. The names are probably familiar: Greenguard, Green Seal, Energy Star, Environmentally Preferable Products, SmartWood, Green Label Plus,...

  4. alizarine cyanine green: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    print advertising and trade show booths. The names are probably familiar: Greenguard, Green Seal, Energy Star, Environmentally Preferable Products, SmartWood, Green Label Plus,...

  5. Green Building Certification Systems Requirement for New Federal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Building Certification Systems Requirement for New Federal Buildings and Major Renovations of Federal Buildings OIRA Comparison Document Green Building Certification Systems...

  6. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1994-06-28T23:59:59.000Z

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

  7. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1996-03-12T23:59:59.000Z

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

  8. Probing the denatured state ensemble with fluorescence 

    E-Print Network [OSTI]

    Alston, Roy Willis

    2004-09-30T23:59:59.000Z

    To understand protein stability and the mechanism of protein folding, it is essential that we gain a better understanding of the ensemble of conformations that make up the denatured state of a protein. The primary goal of the research described...

  9. Critical tonicity determination of sperm using fluorescent staining and flow cytometry

    SciTech Connect (OSTI)

    Noiles, E.E.; Ruffing, N.A.; Kleinhans, F.W.; Mark, L.A.; Watson, P.F.; Critser, J.K. (Methodist Hospital, Indianapolis, IN (USA)); Horstman, L. (Purdue Univ., Lafayette, IN (USA). School of Veterinary Medicine); Mazur, P. (Oak Ridge National Lab., TN (USA))

    1990-01-01T23:59:59.000Z

    The use of cryopreserved, rather than fresh, mammalian semen for artificial insemination confers several important medical and/or economic advantages. However, current methods for cryopreservation of both human and bovine spermatozoa result in approximately only a 50% survival rate with thawing, obviously reducing the fertilizing capacity of the semen. A primary consideration during the cooling process is to avoid intracellular ice crystal formation with its lethal consequences to the cell. Current techniques achieve this by controlling the cooling rate. Computation of the time necessary for this dehydration, and hence, the cooling rate, is dependent upon knowledge of the water permeability coefficient (L{sub {rho}}) and its activation energy. The fluorophore, 6-carboxyfluoroscein diacetate (CFDA), which is nonfluorescent, readily crosses the intact plasma membrane. Intracellular esterases hydrolyze CFDA to 6-carboxyfluoroscein, a fluorescent, membrane-impermeable fluorophore. Consequently, spermatozoa with intact plasma membranes fluoresce bright green (Garner et. al., 1986), but those with disrupted membranes do not. Therefore, the purpose of this study was to use loss of CFDA fluorescence to determine the osmolality at which 50% of the spermatozoa will swell and lyse (critical tonicity, CT). These data will then be used to determine the L{sub {rho}} and its activation energy for sperm, thus increasing the knowledge available in cellular cryopreservation. 15 refs., 3 figs.

  10. Public goods and private interests: Understanding non-residential demand for green power

    E-Print Network [OSTI]

    Wiser, Ryan H.; Fowlie, Meredith; Holt, Edward A.

    2001-01-01T23:59:59.000Z

    Residential Demand for Green Power Buying Green Power: Survey Results Profiling the Respondents The profile

  11. Ultratrace analysis of transuranic actinides by laser-induced fluorescence

    DOE Patents [OSTI]

    Miller, S.M.

    1983-10-31T23:59:59.000Z

    Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

  12. Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly

    E-Print Network [OSTI]

    Chang, Dongsook

    Bioconjugates of the model red fluorescent protein mCherry and synthetic polymer blocks with different hydrogen bonding functionalities show that the chemistry of the polymer block has a large effect on both ordering ...

  13. City of Dallas Green Building Case Study

    E-Print Network [OSTI]

    Basora, Z.

    2012-01-01T23:59:59.000Z

    of the city?s green building and energy management policies ?Update of green building policy in 2006 to require a minimum compliance of LEED Gold and incorporation of carbon neutrality goals ? Implementation of training programs on sustainability for city... Roofs for slopes 2:12 or less to meet EPA ENERGY STAR, or ? Install a green/vegetated roof that covers at least 50% of the roof area with the remainder following the Cool Roof requirement for slopes 2:12 or less ?Outdoor Lighting restriction 14...

  14. Green Functions of Relativistic Field Equations

    E-Print Network [OSTI]

    Ying-Qiu Gu

    2006-12-20T23:59:59.000Z

    In this paper, we restudy the Green function expressions of field equations. We derive the explicit form of the Green functions for the Klein-Gordon equation and Dirac equation, and then estimate the decay rate of the solution to the linear equations. The main motivation of this paper is to show that: (1). The formal solutions of field equations expressed by Green function can be elevated as a postulate for unified field theory. (2). The inescapable decay of the solution of linear equations implies that the whole theory of the matter world should include nonlinear interaction.

  15. Green House Gases | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.NewofGeothermal Heat AnnualGreen Business infrom TexasGreenGreen

  16. Greening up fossil fuels with carbon sequestration

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.NewofGeothermal Heat AnnualGreen BusinessGreenGovGreening up

  17. Pinning down energy levels | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    levels Pinning down energy levels Released: September 12, 2014 Scientists discover the energy differences behind green fluorescent protein's glow The research begins with (a)...

  18. Meet CMI Researcher Joni Barnes | Critical Materials Institute

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to the development of: a gas phase bioreactor for the removal of nitrogen oxides; a green fluorescent protein based biosensor for the detection of arsenic; heat and acid...

  19. ACADEMIA AND INDUSTRY FOR A GREEN (IT) SOCIETY

    E-Print Network [OSTI]

    Lefèvre, Laurent

    LINKING ACADEMIA AND INDUSTRY FOR A GREEN (IT) SOCIETY Christina Herzog, IRIT, University value e.g: Green hype, Green washing Freedom of Action Approach Search and find e.g: measuring load problems, "old wines in new bottles" High but limits through resources e.g: GreenIT allows breakthrough

  20. Providing Green SLAs in High Performance Computing Clouds

    E-Print Network [OSTI]

    class of green services, in addition to existing (energy-source- oblivious) services. This new class client job specifies a Green SLA, which is the minimum percentage of green energy that must be used of green energy. I. INTRODUCTION It is well known that datacenters consume an enormous amount

  1. The Green Party: an undeserved success? Michel Perottino

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    55 The Green Party: an undeserved success? Michel Perottino The success of the Green Party (Strana this development, although it had quite serious consequences for the development of the Greens, as we shall see in the programme of the Greens over the last two decades have #12;Social Movement and Public Action 56 been

  2. Green's Functions and Their Applications to Quantum Mechanics

    E-Print Network [OSTI]

    Morrow, James A.

    Green's Functions and Their Applications to Quantum Mechanics Jeff Schueler June 2, 2011 Contents 1 Green's Functions in Quantum Mechanics and Many-body Theory 8 3.1 Time Independent Green's Fuctions . . . . . . . . . . . . . . 8 3.2 Solving the Schr¨odinger Equation Using Green's Functions . . 12 4 Conclusion 13 1 #12

  3. ON GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS

    E-Print Network [OSTI]

    Shoj, Toshiaki

    ON GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS TOSHIAKI SHOJI Department of Mathematics Science University of Tokyo Noda, Chiba 278­8510, Japan §1. What are the Green functions Green functions are introduced by J.A. Green[G] in 1955, in connection with the representation theory of general linear groups GL

  4. ON GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS

    E-Print Network [OSTI]

    Shoj, Toshiaki

    ON GREEN FUNCTIONS ASSOCIATED TO COMPLEX REFLECTION GROUPS University of Tokyo Noda, Chiba 278-8510, Japan x1. What are the Green functions Green functions are introduced by J.A. Green[G] in 1955, in connection with * *the

  5. Improving Conditions for Green Building Construction in North

    E-Print Network [OSTI]

    Improving Conditions for Green Building Construction in North America QUEST 2012 INTERNATIONAL green building a standard practice throughout North America. · In 2011, the CEC formed the Trilateral Green Building Task Force, comprising North American leaders from the green building industry

  6. Green Building Features Northwest Center for Engineering, Science and Technology

    E-Print Network [OSTI]

    Bertini, Robert L.

    Green Building Features Northwest Center for Engineering, Science and Technology RESOURCE for commercial buildings developed by the U.S. Green Building Council (USGBC) to provide a national consensus in what constitutes a "green" building and to provide market incentives to build green. PSU has received

  7. Green Roof Effect on Arthropod Biodiversity By Caitlin Race

    E-Print Network [OSTI]

    Ginzel, Matthew

    Green Roof Effect on Arthropod Biodiversity By Caitlin Race A green roof is a roof. Green roof technology has become increasingly popular due to several benefits including, insulation, runoff protection, and aesthetic value. This project was designed to see if a green roof would benefit

  8. University AdvAncement Bowling Green State University

    E-Print Network [OSTI]

    Moore, Paul A.

    University AdvAncement Bowling Green State University Office of Alumni and Development Bowling Green State University Mileti Alumni Center Bowling Green, Ohio 43403-0054 advance.bgsu.edu B O W L I N load. As Bowling Green State University progresses toward achieving its vision of being the premier

  9. BOWLING GREEN STATE UNIVERSITY Assurance #A3536-01

    E-Print Network [OSTI]

    Moore, Paul A.

    1 BOWLING GREEN STATE UNIVERSITY Assurance #A3536-01 ANIMAL WELFARE ASSURANCE in accordance Official for animal care and use at Bowling Green State University, hereinafter referred to as Institution by this Institution. "Institution" includes: · Bowling Green State University Main Campus, Bowling Green, OH 43403 o

  10. Green Energy: Advancing Bio-Hydrogen (Presentation)

    SciTech Connect (OSTI)

    Alber, D.

    2007-07-01T23:59:59.000Z

    Developing a model of metabolism linked to H2 production in green algae. Develop tools for parameter discovery and optimization at organism level and advance knowledge of hydrogen-producting photosynthetic organisms.

  11. Challenges in implementing green workforce development training

    E-Print Network [OSTI]

    Yeung, Louise H. (Louise Ho-Yee)

    2013-01-01T23:59:59.000Z

    To meet the labor demands of green economic development, workforce development programs are increasingly training low-income workers for living wage jobs that contribute to environmental services or benefits. Yet, effectively ...

  12. City of Dallas- Green Energy Purchasing

    Broader source: Energy.gov [DOE]

    Furthermore, the Dallas/Fort Worth International Airport is the highest-ranked airport for renewable energy use, separately ranking 7th on the Green Power Partner listing of local government gree...

  13. Microsoft Word - Green Building Certification EA

    Broader source: Energy.gov (indexed) [DOE]

    As required by Section 436(h) of EISA 2007, in 2008 GSA identified the U.S. Green Building Council's Leadership in Energy and Environmental Design (LEED) 5 Silver as...

  14. Generations of green honored with endowments

    E-Print Network [OSTI]

    Liu, Taosheng

    Generations of green honored with endowments Page 5 Michael Serling: A legacy of Support to enhance of knowledge and resources to improve their quality of life and that of future generations." According to Hamm

  15. Exam 2 Green/Form 01

    E-Print Network [OSTI]

    2014-10-12T23:59:59.000Z

    3. On the scantron, fill in your name, section number, student ID. For the test/quiz number, put down 01 for green exam and 02 for orange exam. Sign your name.

  16. Exact fermionic Green's functions from holograpny

    E-Print Network [OSTI]

    Zhong-Ying Fan

    2014-12-15T23:59:59.000Z

    We construct a series of charged dilatonic black holes which share zero entropy in the zero temperature limit using Einstein-Maxwell-Dilaton theories. In these black holes, the wave functions and the Green's functions of massless fermions can be solved exactly in terms of special functions in the phase space of $(\\omega,k)$. We observe that for sufficiently large charge, there are many poles in the Green's function with vanishing $\\omega$, which strongly signifies that Fermi surfaces exist in these holographic systems. The new distinguishing properties of the Green's function arising in these systems were illustrated with great details. We also study the poles motion of the Green's function for arbitrary (complex) frequency. Our analytic results provide a more realistic and elegant approach to study strongly correlated fermionic systems using gauge/gravity duality.

  17. The economics of investing in green buildings

    E-Print Network [OSTI]

    Rizk, Charbel Maroun

    2010-01-01T23:59:59.000Z

    This thesis discusses economics of green buildings. The need to reduce greenhouse gases emissions became clear. Buildings account for a large part of the greenhouse gases emissions, changing the atmosphere's composition. ...

  18. San Diego County- Green Building Program

    Broader source: Energy.gov [DOE]

    The County of San Diego has a Green Building Incentive Program designed to promote the use of resource efficient construction materials, water conservation and energy efficiency in new and...

  19. CHARGING STATION FOR ELECTRIC VEHICLES GREEN PARKING

    E-Print Network [OSTI]

    Vellend, Mark

    CHARGING STATION FOR ELECTRIC VEHICLES P 3 P 3 P 6 GREEN PARKING UNIVERSITÉ DE SHERBROOKE YELLOW (CAR-POOLING) PERMITS HOSPITAL PARKING PARKING-PERMIT DISPENSERS RESERVED DISABLED PARKING PLACES ONE

  20. Chandler- Expedited Plan Review for Green Buildings

    Broader source: Energy.gov [DOE]

    The mayor and city council of Chandler, AZ adopted Resolution 4199 in June 2008, establishing incentives for green building in the private sector. Permit applications for buildings registered with...