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Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
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We encourage you to perform a real-time search of NLEBeta
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1

Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 A resolution  

NLE Websites -- All DOE Office Websites (Extended Search)

Acta Acta Cryst. (2013). F69, 1335-1338 doi:10.1107/S1744309113030005 1335 Acta Crystallographica Section F Structural Biology and Crystallization Communications ISSN 1744-3091 Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 A ˚ resolution Markus Alahuhta, William S. Adney, Michael E. Himmel and Vladimir V. Lunin* BioSciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA Correspondence e-mail: vladimir.lunin@nrel.gov Received 27 September 2013 Accepted 1 November 2013 PDB reference: family 74 glycoside hydrolase, 4lgn Here, a 1.82 A ˚ resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an R free of 0.196. Structural analysis shows that five related structures

2

Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: Heterologous expression, characterization, and synergy with family 48 cellobiohydrolase  

NLE Websites -- All DOE Office Websites (Extended Search)

Glycoside Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: Heterologous expression, characterization, and synergy with family 48 cellobiohydrolase Xiao-Zhou Zhang a , Noppadon Sathitsuksanoh a,b , Y.-H.P. Zhang a,b,c, * a Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA b Institute for Critical Technology and Applied Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA c BioEnergy Science Center of Department of Energy, Oak Ridge, TN 37831, USA a r t i c l e i n f o Article history: Received 2 December 2009 Received in revised form 26 January 2010 Accepted 28 January 2010 Available online 4 March 2010 Keywords: Clostridium phytofermentans Cellulase Family 9 glycoside hydrolase Processive endoglucanase Synergy a b s t r a c t The glycoside

3

Variants of glycoside hydrolases  

DOE Patents (OSTI)

The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

Teter, Sarah (Davis, CA); Ward, Connie (Hamilton, MT); Cherry, Joel (Davis, CA); Jones, Aubrey (Davis, CA); Harris, Paul (Carnation, WA); Yi, Jung (Sacramento, CA)

2011-04-26T23:59:59.000Z

4

Glycoside hydrolases: Catalytic base/nucleophile diversity  

NLE Websites -- All DOE Office Websites (Extended Search)

Glycoside Glycoside Hydrolases: Catalytic Base/Nucleophile Diversity Thu V. Vuong, David B. Wilson Department of Molecular Biology and Genetics, Cornell University, 458 Biotechnology Building, Ithaca, New York 14850; telephone: 607-255-5706; fax: 607-255-2428; e-mail: dbw3@cornell.edu Received 1 April 2010; revision received 27 May 2010; accepted 2 June 2010 Published online 15 June 2010 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/bit.22838 ABSTRACT: Recent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/ nucleophile. A variety of mechanisms are used to replace this function, including substrate-assisted catalysis, a network of several residues, and the use of non-carboxylate residues or exogenous nucleophiles. Removal of the catalytic base/ nucleophile

5

Annotation and comparative analysis of the glycoside hydrolase genes in Brachypodium distachyon  

Science Conference Proceedings (OSTI)

Background Glycoside hydrolases cleave the bond between a carbohydrate and another carbohydrate, a protein, lipid or other moiety. Genes encoding glycoside hydrolases are found in a wide range of organisms, from archea to animals, and are relatively abundant in plant genomes. In plants, these enzymes are involved in diverse processes, including starch metabolism, defense, and cell-wall remodeling. Glycoside hydrolase genes have been previously cataloged for Oryza sativa (rice), the model dicotyledonous plant Arabidopsis thaliana, and the fast-growing tree Populus trichocarpa (poplar). To improve our understanding of glycoside hydrolases in plants generally and in grasses specifically, we annotated the glycoside hydrolase genes in the grasses Brachypodium distachyon (an emerging monocotyledonous model) and Sorghum bicolor (sorghum). We then compared the glycoside hydrolases across species, both at the whole-genome level and at the level of individual glycoside hydrolase families. Results We identified 356 glycoside hydrolase genes in Brachypodium and 404 in sorghum. The corresponding proteins fell into the same 34 families that are represented in rice, Arabidopsis, and poplar, helping to define a glycoside hydrolase family profile which may be common to flowering plants. Examination of individual glycoside hydrolase familes (GH5, GH13, GH18, GH19, GH28, and GH51) revealed both similarities and distinctions between monocots and dicots, as well as between species. Shared evolutionary histories appear to be modified by lineage-specific expansions or deletions. Within families, the Brachypodium and sorghum proteins generally cluster with those from other monocots. Conclusions This work provides the foundation for further comparative and functional analyses of plant glycoside hydrolases. Defining the Brachypodium glycoside hydrolases sets the stage for Brachypodium to be a monocot model for investigations of these enzymes and their diverse roles in planta. Insights gained from Brachypodium will inform translational research studies, with applications for the improvement of cereal crops and bioenergy grasses.

Tyler, Ludmila [USDA-ARS Western Regional Research Center; Bragg, Jennifer [USDA-ARS Western Regional Research Center; Wu, Jiajie [USDA-ARS Western Regional Research Center; Yang, Xiaohan [ORNL; Tuskan, Gerald A [ORNL; Vogel, John [USDA-ARS Western Regional Research Center

2010-01-01T23:59:59.000Z

6

Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases  

DOE Green Energy (OSTI)

To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenomes of natural microbial biomass decay communities may harbor such enzymes. Our goal was to discover and characterize new glycoside hydrolases (GHases) from microbial biomass decay communities, especially those from unknown or never previously cultivated microorganisms. From the metagenome sequences of an anaerobic microbial community actively decaying poplar biomass, we identified approximately 4,000 GHase homologs. Based on homology to GHase families/activities of interest and the quality of the sequences, candidates were selected for full-length cloning and subsequent expression. As an alternative strategy, a metagenome expression library was constructed and screened for GHase activities. These combined efforts resulted in the cloning of four novel GHases that could be successfully expressed in Escherichia coli. Further characterization showed that two enzymes showed significant activity on p-nitrophenyl-{alpha}-L-arabinofuranoside, one enzyme had significant activity against p-nitrophenyl-{beta}-D-glucopyranoside, and one enzyme showed significant activity against p-nitrophenyl-{beta}-D-xylopyranoside. Enzymes were also tested in the presence of ionic liquids. Metagenomics provides a good resource for mining novel biomass degrading enzymes and for screening of cellulolytic enzyme activities. The four GHases that were cloned may have potential application for deconstruction of biomass pretreated with ionic liquids, as they remain active in the presence of up to 20% ionic liquid (except for 1-ethyl-3-methylimidazolium diethyl phosphate). Alternatively, ionic liquids might be used to immobilize or stabilize these enzymes for minimal solvent processing of biomass.

Li L. L.; van der Lelie D.; Taghavi, S.; McCorkle, S. M.; Zhang, Y.-B.; Blewitt, M. G.; Brunecky, R.; Adney, W. S.; Himmel, M. E.; Brumm, P.; Drinkwater, C.; Mead, D. A.; Tringe, S. G.

2011-08-01T23:59:59.000Z

7

Annotation and comparative analysis of the glycoside hydrolase genes in Brachypodium distachyon  

NLE Websites -- All DOE Office Websites (Extended Search)

Annotation Annotation and comparative analysis of the glycoside hydrolase genes in Brachypodium distachyon Ludmila Tyler 1,2 , Jennifer N Bragg 1† , Jiajie Wu 1,3† , Xiaohan Yang 4 , Gerald A Tuskan 4 , John P Vogel 1* Abstract Background: Glycoside hydrolases cleave the bond between a carbohydrate and another carbohydrate, a protein, lipid or other moiety. Genes encoding glycoside hydrolases are found in a wide range of organisms, from archea to animals, and are relatively abundant in plant genomes. In plants, these enzymes are involved in diverse processes, including starch metabolism, defense, and cell-wall remodeling. Glycoside hydrolase genes have been previously cataloged for Oryza sativa (rice), the model dicotyledonous plant Arabidopsis thaliana, and the fast- growing tree Populus trichocarpa (poplar). To improve our understanding of glycoside hydrolases in plants generally

8

Targeted Discovery of Glycoside Hydrolases from a Switchgrass-Adapted Compost Community  

Science Conference Proceedings (OSTI)

Development of cellulosic biofuels from non-food crops is currently an area of intense research interest. Tailoring depolymerizing enzymes to particular feedstocks and pretreatment conditions is one promising avenue of research in this area. Here we added a green-waste compost inoculum to switchgrass (Panicum virgatum) and simulated thermophilic composting in a bioreactor to select for a switchgrass-adapted community and to facilitate targeted discovery of glycoside hydrolases. Smallsubunit (SSU) rRNA-based community profiles revealed that the microbial community changed dramatically between the initial and switchgrass-adapted compost (SAC) with some bacterial populations being enriched over 20-fold. We obtained 225 Mbp of 454-titanium pyrosequence data from the SAC community and conservatively identified 800 genes encoding glycoside hydrolase domains that were biased toward depolymerizing grass cell wall components. Of these, ,10percent were putative cellulasesmostly belonging to families GH5 and GH9. We synthesized two SAC GH9 genes with codon optimization for heterologous expression in Escherichia coli and observed activity for one on carboxymethyl cellulose. The active GH9 enzyme has a temperature optimum of 50uC and pH range of 5.5 to 8 consistent with the composting conditions applied. We demonstrate that microbial communities adapt to switchgrass decomposition using simulated composting condition and that full-length genes can be identified from complex metagenomic sequence data, synthesized and expressed resulting in active enzyme.

Reddy, Amitha; Allgaier, Martin; Park, Joshua I.; Ivanoval, Natalia; Dhaeseleer, Patrik; Lowry, Steve; Sapra, Rajat; Hazen, Terry C.; Simmons, Blake A.; VanderGheynst, Jean S.; Hugenholtz, Philip

2011-05-11T23:59:59.000Z

9

Glycoside hydrolase inventory drives plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus  

NLE Websites -- All DOE Office Websites (Extended Search)

Glycoside Glycoside Hydrolase Inventory Drives Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Amy L. VanFossen, Inci Ozdemir, Samantha L. Zelin, Robert M. Kelly Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905; telephone: 919-515-6396; fax: 919-515-3465; e-mail: rmkelly@eos.ncsu.edu Received 15 November 2010; revision received 22 January 2011; accepted 1 February 2011 Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/bit.23093 ABSTRACT: The genome of Caldicellulosiruptor saccharoly- ticus encodes a range of glycoside hydrolases (GHs) that mediate plant biomass deconstruction by this bacterium. Two GH-based genomic loci that appear to be central to the hydrolysis of hemicellulosic and cellulosic substrates

10

Colloid-based multiplexed method for screening plant biomass-degrading glycoside hydrolase activities in microbial communities  

Science Conference Proceedings (OSTI)

The enzymatic hydrolysis of long-chain polysaccharides is a crucial step in the conversion of biomass to lignocellulosic biofuels. The identification and characterization of optimal glycoside hydrolases is dependent on enzyme activity assays, however existing methods are limited in terms of compatibility with a broad range of reaction conditions, sample complexity, and especially multiplexity. The method we present is a multiplexed approach based on Nanostructure-Initiator Mass Spectrometry (NIMS) that allowed studying several glycolytic activities in parallel under diverse assay conditions. Although the substrate analogs carried a highly hydrophobic perfluorinated tag, assays could be performed in aqueous solutions due colloid formation of the substrate molecules. We first validated our method by analyzing known {beta}-glucosidase and {beta}-xylosidase activities in single and parallel assay setups, followed by the identification and characterization of yet unknown glycoside hydrolase activities in microbial communities.

Reindl, W.; Deng, K.; Gladden, J.M.; Cheng, G.; Wong, A.; Singer, S.W.; Singh, S.; Lee, J.-C.; Yao, J.-S.; Hazen, T.C.; Singh, A.K; Simmons, B.A.; Adams, P.D.; Northen, T.R.

2011-05-01T23:59:59.000Z

11

Fungal glycoside hydrolases for saccharification of lignocellulose: outlook for new discoveries fueled by genomics and functional studies  

DOE Green Energy (OSTI)

Genome sequencing of a variety of fungi is a major initiative currently supported by the Department of Energys Joint Genome Institute. Encoded within the genomes of many fungi are upwards of 200+ enzymes called glycoside hydrolases (GHs). GHs are known for their ability to hydrolyze the polysaccharide components of lignocellulosic biomass. Production of ethanol and next generation biofuels from lignocellulosic biomass represents a sustainable route to biofuels production. However this process has to become more economical before large scale operations are put into place. Identifying and characterizing GHs with improved properties for biomass degradation is a key factor for the development of cost effective processes to convert biomass to fuels and chemicals. With the recent explosion in the number of GH encoding genes discovered by fungal genome sequencing projects, it has become apparent that improvements in GH gene annotation processes have to be developed. This will enable more informed and efficient decision making with regard to selection and utilization of these important enzymes in bioprocess that produce fuels and chemicals from lignocellulosic feedstocks.

Jovanovic, Iva; Magnuson, Jon K.; Collart, Frank R.; Robbertse, Barbara; Adney, William S.; Himmel, Michael E.; Baker, Scott E.

2009-08-01T23:59:59.000Z

12

The Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Family Family 1 Glycoside Hydrolase from Clostridium cellulolyticum H10 is a Cellodextrin Glucohydrolase Wenjin Liu & David R. Bevan & Y.-H. Percival Zhang Received: 29 May 2009 / Accepted: 15 September 2009 # Humana Press 2009 Abstract The only family 1 glycoside hydrolase in Clostridium cellulolyticum H10 (CcGH1) is annotated as a beta-galactosidase but has high sequence homology with many beta- glucosidases. Given the possible importance of beta-glucosidase in cellulose utilization by C. cellulolyticum, the encoding open reading frame Ccel_0374 was cloned and expressed in E. coli as a soluble fusion protein with thioredoxin. After tag cleavage, the purified enzyme had a molecular mass of 52 kDa and was active in dimeric form on a broad range of substrates, including cellobiose, cellotriose, cellotetraose, p-nitrophenyl-beta-glucopyranoside, lactose, and o-nitrophenyl-beta-galactopyranoside.

13

New Cellulase Identification Method Holds Promise for Lower-Cost...  

NLE Websites -- All DOE Office Websites (Extended Search)

within the protein family GH48, a key component for degrading lignocellulose for biofuels. Cellulase enzymes, particularly from the glycoside hydrolase family 48 (GH48), are a...

14

Targeted Discovery of Glycoside Hydrolases from a Switchgrass-Adapted Compost Community  

E-Print Network (OSTI)

organic matter into carbon dioxide, water, and stable humus-A, temperature; B, carbon dioxide evolution rates (CER); C,phase (Figure 1A). Carbon dioxide evolution (CER) and oxygen

Reddy, Amitha

2012-01-01T23:59:59.000Z

15

Biochemical Characterization of ?-Xylan Acting Glycoside Hydrolases from the Thermophilic Bacterium Caldicellulosiruptor Saccharolyticus.  

E-Print Network (OSTI)

??Fossil fuels have been the dominant source for energy around the world since the industrial revolution, however, with the increasing demand for energy and decreasing (more)

Cao, Jin

2012-01-01T23:59:59.000Z

16

Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community  

E-Print Network (OSTI)

S2 Correspondence analysis of the compost bioreactor Author2004) Microbial Ecology of Compost. In: Lens P, Hamelers B,composting in a monitored compost bin. J Appl Microbiol 94:

Allgaier, M.

2010-01-01T23:59:59.000Z

17

Targeted Discovery of Glycoside Hydrolases from a Switchgrass-Adapted Compost Community  

E-Print Network (OSTI)

2004) Microbial Ecology of Compost. In: Lens P, Hamelers B,composting in a monitored compost bin. J Appl Microbiol 94:a Switchgrass-Adapted Compost Community Martin Allgaier ,

Reddy, Amitha

2012-01-01T23:59:59.000Z

18

Targeted Discovery of Glycoside Hydrolases from a Switchgrass-Adapted Compost Community  

E-Print Network (OSTI)

biomass feedstocks for biofuel production. Genome Biol 9:expensive steps in biofuel production from lignocellulosicenvisioned for future biofuel production (e.g. switchgrass,

Reddy, Amitha

2012-01-01T23:59:59.000Z

19

Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community  

E-Print Network (OSTI)

biomass feedstocks for biofuel production. Genome Biol 9:expensive steps in biofuel production from lignocellulosicenvisioned for future biofuel production (e.g. switchgrass,

Allgaier, M.

2010-01-01T23:59:59.000Z

20

Community dynamics and glycoside hydrolase activities of thermophilic bacterial consortia adapted to switchgrass  

DOE Green Energy (OSTI)

Industrial-scale biofuel production requires robust enzymatic cocktails to produce fermentable sugars from lignocellulosic biomass. Thermophilic bacterial consortia are a potential source of cellulases and hemicellulases adapted to harsher reaction conditions than commercial fungal enzymes. Compost-derived microbial consortia were adapted to switchgrass at 60 C to develop thermophilic biomass-degrading consortia for detailed studies. Microbial community analysis using small-subunit rRNA gene amplicon pyrosequencing and short-read metagenomic sequencing demonstrated that thermophilic adaptation to switchgrass resulted in low-diversity bacterial consortia with a high abundance of bacteria related to thermophilic paenibacilli, Rhodothermus marinus, and Thermus thermophilus. At lower abundance, thermophilic Chloroflexi and an uncultivated lineage of the Gemmatimonadetes phylum were observed. Supernatants isolated from these consortia had high levels of xylanase and endoglucanase activities. Compared to commercial enzyme preparations, the endoglucanase enzymes had a higher thermotolerance and were more stable in the presence of 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), an ionic liquid used for biomass pretreatment. The supernatants were used to saccharify [C2mim][OAc]-pretreated switchgrass at elevated temperatures (up to 80 C), demonstrating that these consortia are an excellent source of enzymes for the development of enzymatic cocktails tailored to more extreme reaction conditions.

Gladden, J.M.; Allgaier, M.; Miller, C.S.; Hazen, T.C.; VanderGheynst, J.S.; Hugenholtz, P.; Simmons, B.A.; Singer, S.W.

2011-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Annotation and comparative analysis of the glycoside hydrolase genes in Brachypodium distachyon  

E-Print Network (OSTI)

The Joint Genome Institutes Sorghum biocolor genome page. [T, Poliakov A, et al: The Sorghum bicolor genome and themonocotyledonous model) and Sorghum bicolor (sorghum). We

Tyler, Ludmila; Bragg, Jennifer N; Wu, Jiajie; Yang, Xiaohan; Tuskan, Gerald A; Vogel, John P

2010-01-01T23:59:59.000Z

22

Computational Investigation of Glycosylation Effects on a Family 1 Carbohydrate-Binding Module  

DOE Green Energy (OSTI)

Carbohydrate-binding modules (CBMs) are ubiquitous components of glycoside hydrolases, which degrade polysaccharides in nature. CBMs target specific polysaccharides, and CBM binding affinity to cellulose is known to be proportional to cellulase activity, such that increasing binding affinity is an important component of performance improvement. To ascertain the impact of protein and glycan engineering on CBM binding, we use molecular simulation to quantify cellulose binding of a natively glycosylated Family 1 CBM. To validate our approach, we first examine aromatic-carbohydrate interactions on binding, and our predictions are consistent with previous experiments, showing that a tyrosine to tryptophan mutation yields a 2-fold improvement in binding affinity. We then demonstrate that enhanced binding of 3-6-fold over a nonglycosylated CBM is achieved by the addition of a single, native mannose or a mannose dimer, respectively, which has not been considered previously. Furthermore, we show that the addition of a single, artificial glycan on the anterior of the CBM, with the native, posterior glycans also present, can have a dramatic impact on binding affinity in our model, increasing it up to 140-fold relative to the nonglycosylated CBM. These results suggest new directions in protein engineering, in that modifying glycosylation patterns via heterologous expression, manipulation of culture conditions, or introduction of artificial glycosylation sites, can alter CBM binding affinity to carbohydrates and may thus be a general strategy to enhance cellulase performance. Our results also suggest that CBM binding studies should consider the effects of glycosylation on binding and function.

Taylor, C. B.; Talib, M. F.; McCabe, C.; Bu, L.; Adney, W. S.; Himmel, M. E.; Crowley, M. F.; Beckham, G. T.

2012-01-27T23:59:59.000Z

23

Colloid-based multiplexed method for screening plant biomass-degrading glycoside hydrolase activities in microbial communities  

E-Print Network (OSTI)

in the conversion of biomass to lignocellulosic biofuels.from lignocellulosic biomass (Blanch et al. , 2008): long-in the degradation of biomass. RESULTS NIMS analysis of

Reindl, W.

2012-01-01T23:59:59.000Z

24

TECHNOLOGY DEMONSTRATION UNDERWATER HYDROLASING PHASE 0 & 1 & 2 TECHNICAL REPORT  

Science Conference Proceedings (OSTI)

From September 10 through December 17th, 2003, S.A.Robotics executed Phases 0, I, and II of the Technology Demonstration - Underwater Hydrolasing. Phase 0 was performed at the S.A.Robotics facility in Loveland, Colorado, while Phases I and II were performed at the Hanford K-Basin East Site. The purpose of the demonstrations was to show (1) underwater hydrolasing is a feasible method of removing contaminated concrete underwater to a required depth, (2) the hydrolasing head could be controlled during operation, (3) the depth of contamination in the concrete structure could be accurately measured, and (4) a characterization of the waste stream during hydrolasing activities could be recorded. Video monitoring was also used during all demonstrations. All phases of the demonstration were completed and deemed a success by both the observers and the demonstration team. Single and multiple passes were made using variable cutting rates, different stand-off distances were tested, and stationary cuts were executed. Hot and cold hyrdolasing was performed with radiological and depth scans of the affected surfaces. Specially designed equipment was installed and operated within the contaminated environment of 100-K East Basin. Separate results are documented below by phase. The Phase II radiological demonstration was performed to determine the feasibility of underwater hydrolasing technology for decontamination of the DOE spent fuel basins at Hanford 100-K area. This project demonstration was conducted at 105 KE Basin with the expectation that, once proven, this technology can be implemented at Hanford and other DOE sites.

CHRONISTER, G.B.

2005-06-08T23:59:59.000Z

25

Molecular Models to Emulate Confinement Effects on the Internal Dynamics of Organophosphorous Hydrolase  

SciTech Connect

The confinement of the metalloenzyme organophosphorous hydrolase in functionalized mesoporous silica (FMS) enhances the stability and increases catalytic specific activity by 200% compared to the enzyme in solution. The mechanism by which these processes take place is not well understood. We have developed two coarse-grain models of confinement to provide insights into how the nanocage environment steers enzyme conformational dynamics towards enhanced stability and enzymatic activity. The structural dynamics of organophosphorous hydrolase under the two confinement models are very distinct from each other. Comparisons of the present simulations show that only one model leads to an accurate depiction of the internal dynamics of the enzyme.

Gomes, Diego Enry B.; Lins, Roberto D.; Pascutti, Pedro G.; Straatsma, TP; Soares, Thereza A.

2008-09-28T23:59:59.000Z

26

jbc.M112.405720.full.pdf  

NLE Websites -- All DOE Office Websites (Extended Search)

cellulase in the glycosyl hydrolase cellulase in the glycosyl hydrolase family 48 1 Sequence, Structure, and Evolution of Cellulases in the Glycoside Hydrolase Family 48 Leonid O. Sukharnikov a,1,2 , Markus Alahuhta a,1,3 , Roman Brunecky 1,3 , Amit Upadhyay 1,2 , Michael E. Himmel 1,3 , Vladimir V. Lunin 1,3,b and Igor B. Zhulin 1,2,b 1 BioEnergy Science Center and 2 Joint Institute for Computational Sciences, University of Tennessee - Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA 3 Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA Running title: Defining cellulase in the glycoside hydrolase family 48 a L.O.S. and M.A. contributed equally to this work b To whom correspondence should be addressed: Igor B. Zhulin, Email: joulineib@ornl.gov or Vladimir V. Lunin, Email: vladimir.lunin@nrel.gov

27

A novel meta-cleavage product hydrolase from Flavobacterium sp. ATCC27551  

Science Conference Proceedings (OSTI)

The organophosphate degrading (opd) gene cluster of plasmid pPDL2 of Flavobacterium sp. ATCC27551 contains a novel open-reading frame, orf243. This was predicted to encode an {alpha}/{beta} hydrolase distantly related to the meta-fission product (MFP) hydrolases such as XylF, PhnD, and CumD. By homology modeling Orf243 has most of the structural features of MFP hydrolases including the characteristic active site catalytic triad. The purified protein (designated MfhA) is a homotetramer and shows similar affinity for 2-hydroxy-6-oxohepta-2,4-dienoate (HOHD), 2-hydroxymuconic semialdehyde (HMSA), and 2-hydroxy-5-methylmuconic semialdehyde (HMMSA), the meta-fission products of 3-methyl catechol, catechol, and 4-methyl catechol. The unique catalytic properties of MfhA and the presence near its structural gene of cis-elements required for transposition suggest that mfhA has evolved towards encoding a common hydrolase that can act on meta-fission products containing either aldehyde or ketone groups.

Khajamohiddin, Syed [Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046 (India); Babu, Pakala Suresh [Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046 (India); Chakka, Deviprasanna [Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046 (India); Merrick, Mike [Department of Molecular Microbiology, John Innes Centre, NR4 7UH Norwich (United Kingdom); Bhaduri, Anirban [National Centre for Biological Sciences, Tata Institute of Fundamental Research, UAS-GKVK Campus, Bangalore 560065 (India); Sowdhamini, Ramanathan [National Centre for Biological Sciences, Tata Institute of Fundamental Research, UAS-GKVK Campus, Bangalore 560065 (India); Siddavattam, Dayananda [Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046 (India)]. E-mail: sdsl@uohyd.ernet.in

2006-12-22T23:59:59.000Z

28

Available Technologies: NIMS-based Multiplexed Enzymatic Assays  

Can be used directly with crude environmental samples; ... biomass-degrading glycoside hydrolase activities in microbial communities, Energy & En ...

29

Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus  

DOE Patents (OSTI)

The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Adney, William S. (Golden, CO); Vinzant, Todd B. (Golden, CO); Ding, Shih-You (Golden, CO); Himmel, Michael E. (Golden, CO)

2011-04-26T23:59:59.000Z

30

Thermal tolerant avicelase from Acidothermus cellulolyticus  

DOE Patents (OSTI)

The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Ding, Shi-You (Golden, CO); Adney, William S. (Golden, CO); Vinzant, Todd B. (Golden, CO); Himmel, Michael E. (Littleton, CO)

2008-04-29T23:59:59.000Z

31

Thermal tolerant avicelase from Acidothermus cellulolyticus  

DOE Patents (OSTI)

The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Ding, Shi-You (Golden, CO); Adney, William S. (Golden, CO); Vinzant, Todd B. (Golden, CO); Himmel, Michael E. (Littleton, CO)

2009-05-26T23:59:59.000Z

32

HYDROLASING OF CONTAMINATED UNDERWATER BASIN SURFACES AT THE HANFORD K-AREA  

SciTech Connect

This paper discusses selecting and Implementing hydrolasing technology to reduce radioactive contamination in preparing to dispose of the K Basins; two highly contaminated concrete basins at the Hanford Site. A large collection of spent nuclear fuel stored for many years underwater at the K Basins has been removed to stable, dry, safe storage. Remediation activities have begun for the remaining highly contaminated water, sludge, and concrete basin structures. Hydrolasing will be used to decontaminate and prepare the basin structures for disposal. The U. S. Department of Energy's (DOE) Hanford Site is considered the world's largest environmental cleanup project. The site covers 1,517 Km{sup 2} (586 square miles) along the Columbia River in an arid region of the northwest United States (U.S.). Hanford is the largest of the US former nuclear defense production sites. From the World War II era of the mid-1940s until the late-1980s when production stopped, Hanford produced 60 percent of the plutonium for nuclear defense and, as a consequence, produced a significant amount of environmental pollution now being addressed. Spent nuclear fuel was among the major challenges for DOE's environmental cleanup mission at Hanford. The end of production left Hanford with about 105,000 irradiated, solid uranium metal fuel assemblies--representing approximately 2,100 metric tons (80 percent of DOE's spent nuclear fuel). The fuel was ultimately stored in the K Basins water-filled, concrete basins attached to Hanford's K East (KE) and K West (KW) reactors. K Basin's fuel accounted for 95 percent of the total radioactivity in Hanford's former reactor production areas. Located about 457 meters (500 yards) from the Columbia River, the K Basins are two indoor, rectangular structures of reinforced concrete; each filled with more than 3.8 million liters (one million gallons) of water that has become highly contaminated with long-lived radionuclides. At the KW Basin, fuel was packaged and sealed in canisters. At the KE Basin, fuel was stored in open canisters that were exposed to water in the basin. The irradiated spent nuclear fuel corroded during long-term, wet storage; resulting in thousands of fuel assemblies becoming severely corroded and/or damaged. Corrosion, especially in the KE Basin, contributed to the formation of a layer of radioactive sludge in the basins. Sludge removal is now progressing and will be followed by dewatering and dispositioning the concrete structures. The DOE Richland Operations Office (RL) has given Fluor Hanford Inc./Fluor Government Group (Fluor) the task of preparing Hanford's K Basins for decontamination and disposal. Prior to dewatering, hydrolasing will be used to decontaminate the basin surfaces to prepare them for disposal. By removing highly contaminated surface layers of concrete, hydrolasing will be used to meet the dose objectives for protecting workers and complying with regulations for transporting demolition debris. Fluor has innovated, tested, and planned the application of the hydrolasing technology to meet the challenge of decontaminating highly radioactive concrete surfaces underwater. Newly existing technology is being adapted to this unique challenge.

CHRONISTER, G.B.

2005-06-14T23:59:59.000Z

33

BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS The  

NLE Websites -- All DOE Office Websites (Extended Search)

The The noncellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity Xiao-Zhou Zhang & Zuoming Zhang & Zhiguang Zhu & Noppadon Sathitsuksanoh & Yunfeng Yang & Y.-H. Percival Zhang Received: 21 July 2009 / Revised: 29 August 2009 / Accepted: 31 August 2009 / Published online: 15 October 2009 # Springer-Verlag 2009 Abstract Family 48 glycoside hydrolases (cellobiohydro- lases) are among the most important cellulase components for crystalline cellulose hydrolysis mediated by cellulolytic bacteria. Open reading frame (Cphy_3368) of Clostridium phytofermentans ISDg encodes a putative family 48 glycoside hydrolase (CpCel48) with a family 3 cellulose- binding module. CpCel48 was successfully expressed as two soluble intracellular forms with or without a C-terminal

34

The recombinant expression and potential applications of bacterial organophosphate hydrolase in Zea mays L.  

E-Print Network (OSTI)

Organophosphate hydrolase (OPH, EC 3.1.8.1) is a bacterial enzyme with a broad spectrum of potential substrates that include organophosphorus pesticides, herbicides, and chemical warfare agents. OPH has been expressed successfully in bacterial, fungal, and insect cell culture systems; however, none of these systems produces amounts of enzyme suitable for applications outside of the research laboratory. Therefore, a transgenic Zea mays L. (maize) system was developed to express OPH as an alternate to the current OPH expression systems. The bacterial gene encoding the OPH protein was optimized for transcriptional and translational expression in maize. The optimized gene was inserted into the maize genome under the control of embryo specific, endosperm specific, and constitutive plant promoters. Select transformants were analyzed for the expression of OPH. Expression was observed in the seeds of plants transformed with each of the three constructs with the highest expression observed with the embryo specific and constitutive promoter constructs. The highest OPH expressing lines of transgenic maize had expression levels higher than those reported for the E. coli expression system. OPH was purified from transgenic maize seed and analyzed for posttranslational modification and kinetic properties. OPH was observed to undergo a glycosylation event when expressed in maize that yielded at least two forms of OPH homogolous dimer. The glycosylated form of OPH bound tightly to the Concanavalin A sepharose and remained active after months of storage at room temperature. OPH activity was checked against a number of organophosphate herbicides. Enzymatic activity was observed against the herbicide Amiprophos-methyl and kinetic properties were measured. Enzymatic activity was also tested against the organophosphate Haloxon. Transgenic maize callus, leaf, and seed tissue could be screened for the presence of the optimized opd gene by enzymatic activity. Comparison of the growth of transgenic and control callus on media containing organophosphates showed that the transgenic callus was resistant to the herbicidal effects of haloxon. Transgenic plants expressing OPH were also resistant to the herbicide bensulide when compared to control plants. This indicates that OPH can be used as a screenable marker in plant systems and may be a potential scorable marker system as well.

Pinkerton, Terrence Scott

2003-05-01T23:59:59.000Z

35

Thermal Tolerant Cellulase from Acidothermus Cellulolyticus  

DOE Patents (OSTI)

The invention provides a thermal tolerant cellulase that is a member of the glycoside hydrolase family. The invention further discloses this cellulase as GuxA. GuxA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified GuxA. Methods of making and using GuxA polypeptides, including fusions, variants, and derivatives, are also disclosed.

Ding, S. Y.; Adney, W. S.; Vinzant, T. B.; Himmel, M. E.; Decker, S. R.

2006-06-13T23:59:59.000Z

36

Family History and Offspring  

NLE Websites -- All DOE Office Websites (Extended Search)

Family History and Offspring Name: Chad Location: NA Country: NA Date: NA Question: Does a family's history in any way determine the chances of a child being born male or...

37

Efficient haplotyping for families  

E-Print Network (OSTI)

Hapi is a novel dynamic programming algorithm for haplotyping nuclear families that outperforms contemporary family-based haplotyping algorithms. Haplotypes are useful for mapping and identifying genes which cause and ...

Williams, Amy Lynne, Ph.D. Massachusetts Institute of Technology

2010-01-01T23:59:59.000Z

38

The Figwort Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Figwort Family Figwort Family Nature Bulletin No. 564-A April 26, 1975 Forest Preserve District of Cook County George W. Dunne, President Roland F. Eisenbeis, Supt. of Conservation THE FIGWORT FAMILY The Figwort Family, also known as the Foxglove Family, includes many plants with such strikingly different flowers or such different habits of growth that they scarcely seem to be relatives. The weirdly shaped blossoms of several kinds suggest, if you are fanciful, the faces of animals: some with fearsome open jaws, others with gaping flabby lips or bulging throats. Hence, among them, there are plants with such colorful names as Snapdragon, Turtlehead, Monkey Flower, Little Elephants, Owl's Clover and Pelican Flower. The Figwort, from which the family gets not only a common name but also its scientific name, is a woodland plant with inconspicuous flowers that was once supposed to possess a cure for scrofula. Most members of this family have bitter juice; and several do have medicinal, narcotic or poisonous properties.

39

The Sunfish Family  

NLE Websites -- All DOE Office Websites (Extended Search)

grandmothers as well as wealthy sportsmen -- the sunfish and their near relatives, the black bass and the crappies, would be elected the First Family of American waters. Because...

40

The Minnow Family  

NLE Websites -- All DOE Office Websites (Extended Search)

THE MINNOW FAMILY The word "minnow" is used loosely to mean almost any small fish. Some people even seem to think that all minnows are the young of larger kinds of...

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Family Bargaining and Welfare  

E-Print Network (OSTI)

Jo Bane. (1985). "The Impact of AFDC on Family Structure andunable to detect evidence that AFDC generosity is associatedWinkler (1994). "State AFDC Rules Regarding the Treatment of

Rubalcava, Luis; Thomas, Duncan

2000-01-01T23:59:59.000Z

42

Family Programs | Careers at Brookhaven  

NLE Websites -- All DOE Office Websites (Extended Search)

Careers at Brookhaven Home For Job Seekers Job List Life at Brookhaven Benefits Family Programs Recreation & Fitness Why Brookhaven? For New Hires For Employees Family Programs...

43

The Mint Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Mint Family Mint Family Nature Bulletin No. 432-A November 6, 1971 Forest Preserve District of Cook County George W. Dunne, President Roland F. Eisenbeis, Supt. of Conservation THE MINT FAMILY The Mint Family, in addition to the true mints -- such as Spearmint used in flavoring chewing gum and the mint sauce traditionally served with roast lamb -- contains over 3000 species. Almost all of them are plants with square stems and opposite pairs or whorls of leaves at intervals along the stems. Most of them have a distinctive fragrant or pungent odor, due to volatile oils contained in glands or sacs in the leaves and other parts. Through the ages, the mints have been variously used by man for medicinal purposes and flavorings. Lavender, rosemary and patchouli are perfumes obtained from members of this family. The lemon-scented leaves of Bergamot, one of the true mints, furnish a fragrant oil also used in perfumes. A surprising number of the savories or kitchen herbs used in cooking are plants belonging to this aromatic tribe: sage, thyme, germander, marjoram, the basils, summer savory and hyssop.

44

The Nightshade Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Nightshade Family Nightshade Family Nature Bulletin No. 460-A June 3, 1972 Forest Preserve District of Cook County George W. Dunne, President Roland F. Eisenbeis, Supt. of Conservation THE NIGHTSHADE FAMILY In our forest preserves we frequently find a vine-like plant, with long slender woody stems, twining or climbing at the base of a big tree. It is also found next to buildings, along fence rows and on ditch banks. It has modest clusters of blue 5-lobed flowers with yellow centers, and these are followed in mid-summer by little scarlet berries, with thin transparent skins. that look like tiny tomatoes. They should not be eaten, because they are mildly poisonous. This is the Climbing or Bittersweet Nightshade introduced from Europe but now naturalized and widespread in this country.

45

The Mustard Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Mustard Family Mustard Family Nature Bulletin No. 228-A April 30, 1966 Forest Preserve District of Cook County Seymour Simon, President Roland F. Eisenbeis, Supt. of Conservation THE MUSTARD FAMILY Mustard has long been used as a condiment, and since Biblical times for its medicinal values in plasters, footbaths, and as an emetic. Commercial mustard is obtained from two kinds -- one having white seed, of which the best grades are grown in England and the Netherlands; the other having black seeds, mostly cultivated in California and Kentucky. The tiny seeds, perhaps 250,000 per pound, are ground to powder after their oil has been extracted in presses. Table mustard, usually a blend of the black for aroma and the white for pungency, is prepared by adding salt, spices and vinegar, although gourmets prefer stale beer.

46

Rational design of organophosphorus hydrolase for the degradation and detection of neurotoxic pesticides and chemical warfare agents  

E-Print Network (OSTI)

It is critical to consider the balance between the catalytic capabilities of an enzyme and the inherent structural stability of the protein when developing enzymes for specific applications. Rational site directed mutagenesis has been used to explore the role of residues 254 and 257 in the global stability and catalytic specificities of organophosphorus hydrolase (OPH, EC 3.1.8.1). Substitution of residues H254 and H257, which are located near the active site, had a marked effect on both the global stability and substrate specificity of the enzyme. For example, the for the double mutation CoTG??2+ H254R H257L (RL) enzyme variant was 19.6 kcal/mol, 5.7 kcal/mol less than that of the wild type enzyme. At the same time, the altered enzyme was catalytically more effective against VX and VR (Russian VX), as compared to the wild type enzyme. Limited proteolysis verified the importance of residues 254 and 257 for functional stability, evidenced by enhanced resistance to irreversible unfolding associated with thermal denaturation. It has been possible to construct third generation OPH variants, which are more stable than the wild type enzyme, with a 10 ?°C increase in the apparent melting temperature (TM app), yet retained desirable catalytic properties. It appeared that aromatic stacking and cation-Ï? interactions involving near active site residues not only affected activity but significantly contributed to the chemical and thermal stability of OPH. Rational design was used to develop an enzyme with an optimized orientation on a catalytically active biosensor surface. In these studies, lysine side chains located on the surface of OPH were used to create attachment sites to a surface plasmon resonance sensor resulting in an ensemble of enzyme orientations. Some of these orientations could be functionally restrictive if the active site is oriented toward the sensor surface. Substitution of a lysine near the active site resulted in 20% more activity with 53% less enzyme immobilized, thus increasing the specific activity of the decorated surface 2.5 fold.

Reeves, Tony Elvern

2003-05-01T23:59:59.000Z

47

Higgs family symmetry and supersymmetry  

E-Print Network (OSTI)

In this thesis we investigate building models of family symmetry that give the Higgs fields family structure. We construct several models, starting with 2 generation models then moving onto 3 generation models. These models ...

Patt, Brian Lawrence

2006-01-01T23:59:59.000Z

48

Mutational, Structural, and Kinetic Evidence for a Dissociative Mechanism in the GDP-mannose Mannosyl Hydrolase Reaction  

SciTech Connect

GDP-mannose hydrolase (GDPMH) catalyzes the hydrolysis of GDP-{alpha}-D-sugars by nucleophilic substitution with inversion at the anomeric C1 atom of the sugar, with general base catalysis by H124. Three lines of evidence indicate a mechanism with dissociative character. First, in the 1.3 Angstrom X-ray structure of the GDPMH-Mg{sup 2+}-GDP{center_dot}Tris{sup +} complex, the GDP leaving group interacts with five catalytic components: R37, Y103, R52, R65, and the essential Mg{sup 2+}. As determined by the effects of site-specific mutants on k{sub cat}, these components contribute factors of 24-, 100-, 309-, 24-, and {ge}10{sup 5}-fold, respectively, to catalysis. Both R37 and Y103 bind the {beta}-phosphate of GDP and are only 5.0 Angstroms apart. Accordingly, the R37Q/Y103F double mutant exhibits partially additive effects of the two single mutants on k{sub cat}, indicating cooperativity of R37 and Y103 in promoting catalysis, and antagonistic effects on K{sub m}. Second, the conserved residue, D22, is positioned to accept a hydrogen bond from the C2-OH group of the sugar undergoing substitution at C1, as was shown by modeling an {alpha}-D-mannosyl group into the sugar binding site. The D22A and D22N mutations decreased k{sub cat} by factors of 10{sup 2.1} and 10{sup 2.6}, respectively, for the hydrolysis of GDP-{alpha}-D-mannose, and showed smaller effects on K{sub m}, suggesting that the D22 anion stabilizes a cationic oxocarbenium transition state. Third, the fluorinated substrate, GDP-2F-{alpha}-D-mannose, for which a cationic oxocarbenium transition state would be destabilized by electron withdrawal, exhibited a 16-fold decrease in k{sub cat} and a smaller, 2.5-fold increase in K{sub m}. The D22A and D22N mutations further decreased the k{sub cat} with GDP-2F-{alpha}-D-mannose to values similar to those found with GDP-{alpha}-D-mannose, and decreased the K{sub m} of the fluorinated substrate. The choice of histidine as the general base over glutamate, the preferred base in other Nudix enzymes, is not due to the greater basicity of histidine, since the pK{sub a} of E124 in the active complex (7.7) exceeded that of H124 (6.7), and the H124E mutation showed a 10{sup 2.2}-fold decrease in k{sub cat}and a 4.0-fold increase in K{sub m} at pH 9.3. Similarly, the catalytic triad detected in the X-ray structure (H124---Y127---P120) is unnecessary for orienting H124, since the Y127F mutation had only 2-fold effects on k{sub cat} and K{sub m} with either H124 or E124 as the general base. Hence, a neutral histidine rather than an anionic glutamate may be necessary to preserve electroneutrality in the active complex.

Xia,Z.; Azurmendi, H.; lairson, L.; Withers, S.; Gabelli, S.; Bianchet, M.; Amzel, L.; Mildvan, A.

2005-01-01T23:59:59.000Z

49

Thermal tolerant mannanase from acidothermus cellulolyticus  

DOE Patents (OSTI)

The invention provides a thermal tolerant mannanase that is a member of the glycoside hydrolase family. The invention further discloses this mannanase as ManA. ManA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified ManA. Methods of making ManA polypeptides, including fusions, variants, and derivatives, are also disclosed. Methods of using mannanase A, including for the processing of food and for use in food stuffs as bulking agents and the like, are also disclosed.

Ding, Shi-You (Golden, CO); Adney, William S. (Golden, CO); Vinzant, Todd B. (Golden, CO); Himmel, Michael E. (Littleton, CO)

2006-09-26T23:59:59.000Z

50

Early Education Services Family Handbook  

E-Print Network (OSTI)

Early Education Services Family Handbook A Partnership in Caring Effective January 2013 Early an experience of trust, caring, understanding and learning for us all. This handbook provides important

California at Santa Cruz, University of

51

Early Education Services Family Handbook  

E-Print Network (OSTI)

Early Education Services Family Handbook A Partnership in Caring Effective July 2012 Early an experience of trust, caring, understanding and learning for us all. This handbook provides important

California at Santa Cruz, University of

52

Family Member Definitions | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Memorandum 21 clarifies the definition and application of family member in our directives and services Family Members Responsible Contacts Bruce Murray HR Policy Advisor...

53

Work Family Community, Human Resources  

NLE Websites -- All DOE Office Websites (Extended Search)

Links Links HR Homepage Division Calendar Diversity Office Occupational Medicine Employment Publications & Resources About HROM Contact List Organizational Chart (PDF) Have Questions? Feedback Form Core Values Mission, Vision, & Goals Other Information BNL Site Index Can't View PDFs? Family Friendly Programs Adoption Assistance Program Benefits Blood Drives Brookhaven Employee's Recreation Association (BERA) Brookhaven Retired Employee Association (BREA) Brookhaven Women in Science (BWIS) BSA Scholarship Child Care Council of Suffolk Child Development Center Domestic Partner Benefits Elder Care Resources Employee Assistance Program (EAP) Employer Assisted Housing Program English for Speakers of Other Languages Family and Medical Leave Act (FMLA) Flexible Spending Accounts (Medical and Dependent Care)

54

Search for the fourth standard model family  

Science Conference Proceedings (OSTI)

Existence of the fourth family follows from the basics of the standard model (SM) and the actual mass spectrum of the third family fermions. We discuss possible manifestations of the fourth SM family at existing and future colliders. The LHC and Tevatron potentials to discover the fourth SM family have been compared. The scenario with dominance of the anomalous decay modes of the fourth-family quarks has been considered in detail.

Sahin, M.; Sultansoy, S.; Turkoz, S. [TOBB University of Economics and Technology, Physics Division, Ankara (Turkey); TOBB University of Economics and Technology, Physics Division, Ankara (Turkey) and Institute of Physics, National Academy of Sciences, Baku (Azerbaijan); Ankara University, Department of Physics, Ankara (Turkey)

2011-03-01T23:59:59.000Z

55

NREL airfoil families for HAWTs  

DOE Green Energy (OSTI)

The development of special-purpose airfoils for horizontal-axis wind turbines (HAWTs) began in 1984 as a joint effort between the National Renewable Energy Laboratory (NREL), formerly the Solar Energy Research Institute (SERI), and Airfoils, Incorporated. Since that time seven airfoil families have been designed for various size rotors using the Eppler Airfoil Design and Analysis Code. A general performance requirement of the new airfoil families is that they exhibit a maximum lift coefficient (c{sub l,max}) which is relatively insensitive to roughness effects. The airfoil families address the needs of stall-regulated, variable-pitch, and variable-rpm wind turbines. For stall-regulated rotors, better peak-power control is achieved through the design of tip airfoils that restrain the maximum lift coefficient. Restrained maximum lift coefficient allows the use of more swept disc area for a given generator size. Also, for stall-regulated rotors, tip airfoils with high thickness are used to accommodate overspeed control devices. For variable-pitch and variable-rpm rotors, tip airfoils having a high maximum lift coefficient lend themselves to lightweight blades with low solidity. Tip airfoils having low thickness result in less drag for blades having full-span pitch control. Annual energy improvements from the NREL airfoil families are projected to be 23% to 35% for stall-regulated turbines, 8% to 20% for variable-pitch turbines, and 8% to 10% for variable-rpm turbines. The improvement for stall-regulated turbines has been verified in field tests.

Tangler, J L [National Renewable Energy Lab., Golden, CO (United States); Somers, D M [Airfoils Inc., State College, PA (United States)

1995-01-01T23:59:59.000Z

56

Product Binding Varies Dramatically between Processive and Nonprocessive Cellulase Enzymes  

DOE Green Energy (OSTI)

Cellulases hydrolyze {beta}-1,4 glycosidic linkages in cellulose, which are among the most prevalent and stable bonds in Nature. Cellulases comprise many glycoside hydrolase families and exist as processive or nonprocessive enzymes. Product inhibition negatively impacts cellulase action, but experimental measurements of product-binding constants vary significantly, and there is little consensus on the importance of this phenomenon. To provide molecular level insights into cellulase product inhibition, we examine the impact of product binding on processive and nonprocessive cellulases by calculating the binding free energy of cellobiose to the product sites of catalytic domains of processive and nonprocessive enzymes from glycoside hydrolase families 6 and 7. The results suggest that cellobiose binds to processive cellulases much more strongly than nonprocessive cellulases. We also predict that the presence of a cellodextrin bound in the reactant site of the catalytic domain, which is present during enzymatic catalysis, has no effect on product binding in nonprocessive cellulases, whereas it significantly increases product binding to processive cellulases. This difference in product binding correlates with hydrogen bonding between the substrate-side ligand and the cellobiose product in processive cellulase tunnels and the additional stabilization from the longer tunnel-forming loops. The hydrogen bonds between the substrate- and product-side ligands are disrupted by water in nonprocessive cellulase clefts, and the lack of long tunnel-forming loops results in lower affinity of the product ligand. These findings provide new insights into the large discrepancies reported for binding constants for cellulases and suggest that product inhibition will vary significantly based on the amount of productive binding for processive cellulases on cellulose.

Bu, L.; Nimlos, M. R.; Shirts, M. R.; Stahlberg, J.; Himmel, M. E.; Crowley, M. F.; Beckham, G. T.

2012-07-13T23:59:59.000Z

57

On families of phi, Gamma-modules  

E-Print Network (OSTI)

Berger and Colmez (2008) formulated a theory of families of overconvergent tale (?,?)-modules associated to families of p-adic Galois representations over p-adic Banach algebras. In contrast with the classical theory of ...

Kedlaya, Kiran S.

58

Friedberg Numberings of Families of n ...  

Science Conference Proceedings (OSTI)

the family of all c.e. sets, holds for the family of all n-c.e. sets for any n > 2. Second ... Equivalently, a set A ? ? is n-computably enumerable if there is a uniformly...

59

Protect Your Family Brochure (Training and Awareness Material...  

NLE Websites -- All DOE Office Websites (Extended Search)

Family Brochure (Training and Awareness Material) Training and Awareness Material for Cyber Security. How to protect your family. Protect Your Family Brochure (Training and...

60

Defining Eligible Families in Public housing and Welfare: the Traditions, Values, and Legalities of Family Form.  

E-Print Network (OSTI)

??DEFINING ELIGIBLE FAMILIES IN PUBLIC HOUSING AND WELFARE:THE TRADITIONS, VALUES, AND LEGALITIES OF FAMILY FORM By:Charlotte C. Johnson This paper examines how the definition of (more)

Johnson, Charlotte Charlene

2003-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Analysis of designing interleaved ZCZ sequence families  

Science Conference Proceedings (OSTI)

Interleave structure is a well-known period extending method, by which we can extend the period of an original ZCZ sequence family to generate a long period ZCZ sequence family. In this paper, we first present two basic period extending methods: 1. when ...

Jin-Song Wang; Wen-Feng Qi

2006-09-01T23:59:59.000Z

62

The Temporary Assistance for Needy Families Program  

E-Print Network (OSTI)

nberwelf2_ms The Temporary Assistance for Needy Families (TANF) program was created in 1996 from what was previously named the Aid to Families with Dependent Children (AFDC) program. The TANF program is intended to serve low-income families, primarily those with only a single parent present, as did the AFDC program. The TANF program is distinguished from AFDC by strong work requirements, time limits on receipt, options for the provision of noncash assistance, and by a block grant financing structure. This paper reviews the rules of the TANF program and the research that has been conducted on it and on the AFDC program. The Temporary Assistance for Needy Families (TANF) program was created by legislation passed by the U.S. Congress and signed by the President in 1996. The Personal Responsibility and Work Reconciliation Act (PRWORA) created the TANF program out of the preexisting Aid to Families with Dependent Children (AFDC) program, which itself was created

Robert A. Moffitt

2003-01-01T23:59:59.000Z

63

Economic well-being and the family  

E-Print Network (OSTI)

This thesis examines the well-being of families under changing labor market conditions, changes in the legal environment and changes in public policy. The first chapter asks how women's fertility decisions are affected by ...

Perry, Cynthia D

2004-01-01T23:59:59.000Z

64

Essays in population and family economics  

E-Print Network (OSTI)

(cont.) observed in several Western countries. Finally, the third chapter evaluates the labor market effects of public subsidies to families with children. Using variation in the level of benefits provided by a policy ...

Len, Alexis, 1977-

2004-01-01T23:59:59.000Z

65

Single Family Residential Stormwater Management Guidelines  

E-Print Network (OSTI)

stormwater general permit (permit) implements the federal Clean Water Act. The permit is administered by the Washington State Department of Ecology and requires stormwater management for new development and redevelopment projects (including Single Family

Single Family Residential

2012-01-01T23:59:59.000Z

66

Peoples Gas Single Family Direct Install (Illinois)  

Energy.gov (U.S. Department of Energy (DOE))

Owners of single-family homes, condos, townhomes and two-flats may be eligible for a free installation of new programmable thermostats, pipe insulation, showerheads, and faucet aerators through...

67

Investigation of New Families of HTSC Compounds  

Science Conference Proceedings (OSTI)

The discovery of the new family of copper-oxide superconductors has opened an area of exciting new materials science with enormous potential for practical developments in technology and engineering. However, this should not stop the search for new higher temperature or higher current carrying superconductors. This project investigated the doping of copper chloride to achieve superconductivity. Discovery of new families of superconductors may provide us with more power applications.

1995-03-17T23:59:59.000Z

68

California Solar Initiative - Single-Family Affordable Solar...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

California Solar Initiative - Single-Family Affordable Solar Housing (SASH) Program California Solar Initiative - Single-Family Affordable Solar Housing (SASH) Program Eligibility...

69

NNSA Production Office tops Feds Feed Families campaign goal...  

National Nuclear Security Administration (NNSA)

Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > NNSA Production Office tops Feds Feed Families ... NNSA Production Office tops Feds Feed Families...

70

California Solar Initiative - Multi-Family Affordable Solar Housing...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

California Solar Initiative - Multi-Family Affordable Solar Housing (MASH) Program California Solar Initiative - Multi-Family Affordable Solar Housing (MASH) Program < Back...

71

Career development in academic family medicine: An experiential learning approach.  

E-Print Network (OSTI)

??As managed health care increases in appeal, including the positive implications for family practice, the need for the effective career development of family physicians becomes (more)

Sharp, Mary Anne Rainey

1991-01-01T23:59:59.000Z

72

Flavor Violating Lepton Family U(1)$_\\lambda$  

E-Print Network (OSTI)

The Standard Model is extended minimally with a new flavor-violating family symmetry ${\\rm U(1)}_\\lambda$, which acts only on leptons including the right-handed neutrinos. The model is anomaly free with family-dependent ${\\rm U(1)}_\\lambda$ charges, and consistent with the observed neutrino mixing angles. It predicts charged lepton flavor-violating processes mediated by a new gauge boson. Under certain conditions, the smallness of $\\theta_{13}$ of neutrino mixing can be justified in terms of the muon-to-tau mass ratio, at the same time explaining the electron-to-tau large mass hierarchy.

La, HoSeong

2013-01-01T23:59:59.000Z

73

TIGRFAMS: The TIGRFAMs database of protein families  

DOE Data Explorer (OSTI)

TIGRFAMs are protein families based on Hidden Markov Models or HMMs. Use this page to see the curated seed alignmet for each TIGRFam, the full alignment of all family members and the cutoff scores for inclusion in each of the TIGRFAMs. Also use this page to search through the TIGRFAMs and HMMs for text in the TIGRFAMs Text Search or search for specific sequences in the TIGRFAMs Sequence Search.[Copied from the Overview at http://www.jcvi.org/cms/research/projects/tigrfams/overview/] See also TIGRFAMs ordered by the roles they play at http://cmr.jcvi.org/tigr-scripts/CMR/shared/EvidenceList.cgi?ev_type=TIGRFAM&order_type=role.

74

AEM.01400-10v1.pdf  

NLE Websites -- All DOE Office Websites (Extended Search)

Oct 8, 2010 rmk Oct 8, 2010 rmk Phylogenetic, microbiological and glycoside hydrolase diversity within the extremely thermophilic, plant biomass-degrading genus Caldicellulosiruptor 5 Sara E. Blumer-Schuette, Derrick L. Lewis # , and Robert M. Kelly * Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905 # Present address: Novozymes Biologicals, Salem, VA 10 Submitted to: Applied and Environmental Microbiology (June, 2010) Running title: Plant biomass deconstruction by Caldicellulosiruptor species 15 Keywords: Caldicellulosiruptor, extreme thermophile, plant biomass, glycoside hydrolases *Address correspondence to: Robert M. Kelly 20 Department of Chemical and Biomolecular Engineering

75

Fluoride-Mediated Capture of a Noncovalent Bound State of a Reversible Covalent Enzyme Inhibitor: X-ray Crystallographic Analysis of an Exceptionally Potent [alpha]-Ketoheterocycle Inhibitor of Fatty Acid Amide Hydrolase  

SciTech Connect

Two cocrystal X-ray structures of the exceptionally potent {alpha}-ketoheterocycle inhibitor 1 (K{sub i} = 290 pM) bound to a humanized variant of rat fatty acid amide hydrolase (FAAH) are disclosed, representing noncovalently and covalently bound states of the same inhibitor with the enzyme. Key to securing the structure of the noncovalently bound state of the inhibitor was the inclusion of fluoride ion in the crystallization conditions that is proposed to bind the oxyanion hole precluding inhibitor covalent adduct formation with stabilization of the tetrahedral hemiketal. This permitted the opportunity to detect important noncovalent interactions stabilizing the binding of the inhibitor within the FAAH active site independent of the covalent reaction. Remarkably, noncovalently bound 1 in the presence of fluoride appears to capture the active site in the same 'in action' state with the three catalytic residues Ser241-Ser217-Lys142 occupying essentially identical positions observed in the covalently bound structure of 1, suggesting that this technique of introducing fluoride may have important applications in structural studies beyond inhibiting substrate or inhibitor oxyanion hole binding. Key insights to emerge from the studies include the observations that noncovalently bound 1 binds in its ketone (not gem diol) form, that the terminal phenyl group in the acyl side chain of the inhibitor serves as the key anchoring interaction overriding the intricate polar interactions in the cytosolic port, and that the role of the central activating heterocycle is dominated by its intrinsic electron-withdrawing properties. These two structures are also briefly compared with five X-ray structures of {alpha}-ketoheterocycle-based inhibitors bound to FAAH recently disclosed.

Mileni, Mauro; Garfunkle, Joie; Ezzili, Cyrine; Cravatt, Benjamin F.; Stevens, Raymond C.; Boger, Dale L. (Scripps)

2011-11-02T23:59:59.000Z

76

FM radio: family interplay with sonic mementos  

Science Conference Proceedings (OSTI)

Digital mementos are increasingly problematic, as people acquire large amounts of digital belongings that are hard to access and often forgotten. Based on fieldwork with 10 families, we designed a new type of embodied digital memento, the FM Radio. It ... Keywords: audio, mementos, memories, narrative, tangible interaction.

Daniela Petrelli; Nicolas Villar; Vaiva Kalnikaite; Lina Dib; Steve Whittaker

2010-04-01T23:59:59.000Z

77

publication 348-672 Family Nutrition Program  

E-Print Network (OSTI)

publication 348-672 Family Nutrition Program 1 small chicken breast = 3 ounces Healthy PROTEIN, cashews, and more Fish tuna, salmon, and many other types of fish Lean Poultry skinless chicken and turkey Lean Meat lean cuts of beef, pork, and lamb Eggs whole eggs, egg whites, and egg substitute Milk low-fat

Liskiewicz, Maciej

78

The Mork Family Department of Chemical  

E-Print Network (OSTI)

CHE The Mork Family Department of Chemical Engineering and Materials Science #12;Chemical engineers design, control and optimize large-scale chemical, physiochemical and biochemical processes in automotive and space-related industries to materials used in the biomedical and electronics elds. Chemical

Rohs, Remo

79

Assessing systems adaptability to a product family  

Science Conference Proceedings (OSTI)

In many cases, product families are established on top of a successful pilot product. While this approach provides an option to measure many concrete attributes like performance and memory footprint, adequateness and adaptability of the architecture ... Keywords: adaptability, assessment, product line architecture, software product lines

Mika Korhonen; Tommi Mikkonen

2004-07-01T23:59:59.000Z

80

EM Helps Military Families in Need | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Helps Military Families in Need Helps Military Families in Need EM Helps Military Families in Need January 30, 2013 - 12:00pm Addthis Pictured here are the Christmas gifts purchased with donations raised by EM staff for families of Nellis Air Force Base in Las Vegas. Pictured here are the Christmas gifts purchased with donations raised by EM staff for families of Nellis Air Force Base in Las Vegas. LAS VEGAS - Six families from Nellis Air Force Base in Las Vegas recently received nearly $4,000 in donations - funds that helped provide a welcome relief during the holidays. Donations raised by EM staff at the Nevada National Security Site (NNSS) went toward Christmas gifts for the families' 12 collective children, ranging from age 12 months to 8 years old. Donations also provided each family with a $100 gift card to the Nellis Commissary as well as a $100

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Save Energy at This Year's Family Reunion | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Energy at This Year's Family Reunion Energy at This Year's Family Reunion Save Energy at This Year's Family Reunion July 31, 2013 - 10:39am Addthis With a little planning, your family reunion can be both fun and energy-efficient this year. | Photo courtesy of ©iStockphoto/RonTech2000 With a little planning, your family reunion can be both fun and energy-efficient this year. | Photo courtesy of ©iStockphoto/RonTech2000 Christina Stowers Communications Specialist in the Office of Weatherization and Intergovernmental Program How can I participate? Limit the cooking and disposable dishware for an energy-efficient family reunion. Lately I've been hearing a lot of friends and colleagues talking about attending family reunions, and as it turns out, July is National Family Reunion Month (although some commercial enterprises celebrate it in

82

Save Energy at This Year's Family Reunion | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Save Energy at This Year's Family Reunion Save Energy at This Year's Family Reunion Save Energy at This Year's Family Reunion July 31, 2013 - 10:39am Addthis With a little planning, your family reunion can be both fun and energy-efficient this year. | Photo courtesy of ©iStockphoto/RonTech2000 With a little planning, your family reunion can be both fun and energy-efficient this year. | Photo courtesy of ©iStockphoto/RonTech2000 Christina Stowers Communications Specialist in the Office of Weatherization and Intergovernmental Program How can I participate? Limit the cooking and disposable dishware for an energy-efficient family reunion. Lately I've been hearing a lot of friends and colleagues talking about attending family reunions, and as it turns out, July is National Family Reunion Month (although some commercial enterprises celebrate it in

83

Burlington Electric Department - Multi-Family Rental Energy Efficiency...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Efficiency Rebate Program Eligibility Multi-Family Residential Savings For Heating & Cooling Commercial Heating & Cooling Heating Appliances & Electronics Commercial Lighting...

84

On the origin of families of quarks and leptons - predictions for four families  

E-Print Network (OSTI)

The approach unifying all the internal degrees of freedom--proposed by one of us--is offering a new way of understanding families of quarks and leptons: A part of the starting Lagrange density in d(=1+13), which includes two kinds of spin connection fields--the gauge fields of two types of Clifford algebra objects--transforms the right handed quarks and leptons into the left handed ones manifesting in d=1+3 the Yukawa couplings of the Standard model. We study the influence of the way of breaking symmetries on the Yukawa couplings and estimate properties of the fourth family--the quark masses and the mixing matrix, investigating the possibility that the fourth family of quarks and leptons appears at low enough energies to be observable with the new generation of accelerators.

G. Bregar; M. Breskvar; D. Lukman; N. S. Mankoc Borstnik

2007-08-21T23:59:59.000Z

85

A Family of Circular Bargmann Transforms  

E-Print Network (OSTI)

When considering a charged particle evolving in the Poincar\\'e disk under influence of a uniform magnetic field with a strength proportional to +1, we construct for all hyperbolic Landau level \\epsilon^\\gamma_$m$ m = 4m(-m), m 2 Z+ \\[0, /2] a family of coherent states transforms labeled by (,m) and mapping isometrically square integrable functions on the unit circle with respect to the measure sin^\\gamma-2m (\\theta/2) d\\theta onto spaces of bound states of the particle. These transforms are called circular Bargmann transforms.

Zouhair Mouayn

2011-05-22T23:59:59.000Z

86

2013 Feds Feed Families: Your Generosity Counts | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

013 Feds Feed Families: Your Generosity Counts 013 Feds Feed Families: Your Generosity Counts 2013 Feds Feed Families: Your Generosity Counts July 2, 2013 - 5:58pm Addthis (L to R) Kevin Knobloch, DOE Chief of Staff; Fletcher Honemond, Chief Learning Officer; Doug Keeler, Feds Feeds Families National Program Manager; Ella Daniels of the Capital Area Food Bank; Jennifer Hirschorn; Rauland Sharp, 2013 Feds Feeds Families Deputy Champion. | Photo courtesy of Ella Daniels. (L to R) Kevin Knobloch, DOE Chief of Staff; Fletcher Honemond, Chief Learning Officer; Doug Keeler, Feds Feeds Families National Program Manager; Ella Daniels of the Capital Area Food Bank; Jennifer Hirschorn; Rauland Sharp, 2013 Feds Feeds Families Deputy Champion. | Photo courtesy of Ella Daniels. Fletcher Honemond Fletcher Honemond

87

EM Helps Military Families in Need | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

EM Helps Military Families in Need EM Helps Military Families in Need EM Helps Military Families in Need January 30, 2013 - 12:00pm Addthis Pictured here are the Christmas gifts purchased with donations raised by EM staff for families of Nellis Air Force Base in Las Vegas. Pictured here are the Christmas gifts purchased with donations raised by EM staff for families of Nellis Air Force Base in Las Vegas. LAS VEGAS - Six families from Nellis Air Force Base in Las Vegas recently received nearly $4,000 in donations - funds that helped provide a welcome relief during the holidays. Donations raised by EM staff at the Nevada National Security Site (NNSS) went toward Christmas gifts for the families' 12 collective children, ranging from age 12 months to 8 years old. Donations also provided each

88

2013 Feds Feed Families: Your Generosity Counts | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

2013 Feds Feed Families: Your Generosity Counts 2013 Feds Feed Families: Your Generosity Counts 2013 Feds Feed Families: Your Generosity Counts July 2, 2013 - 5:58pm Addthis (L to R) Kevin Knobloch, DOE Chief of Staff; Fletcher Honemond, Chief Learning Officer; Doug Keeler, Feds Feeds Families National Program Manager; Ella Daniels of the Capital Area Food Bank; Jennifer Hirschorn; Rauland Sharp, 2013 Feds Feeds Families Deputy Champion. | Photo courtesy of Ella Daniels. (L to R) Kevin Knobloch, DOE Chief of Staff; Fletcher Honemond, Chief Learning Officer; Doug Keeler, Feds Feeds Families National Program Manager; Ella Daniels of the Capital Area Food Bank; Jennifer Hirschorn; Rauland Sharp, 2013 Feds Feeds Families Deputy Champion. | Photo courtesy of Ella Daniels. Fletcher Honemond Fletcher Honemond

89

A Family of Discrete Magnetically Switchable Nanoballs  

Science Conference Proceedings (OSTI)

The thermal and light-induced magnetic properties of a family of discrete magnetically switchable 'nanoball' species (3 nm in diameter) is reported. The self-assembly of these materials is accomplished by the use of the metallo building block, [Cu([Tp{sup 4-py}])(NCCH{sub 3})] ([Tp{sup 4-py}]=tris-[3-(4{prime}-pyridyl)pyrazol-1-yl]hydroborate), combined with a [Fe(NCX){sub 2}] (X = S, Se and BH{sub 3}) species. We previously showed that the thiocyanate analogue (Fe(NCS)-nano) undergoes a thermal and light-induced spin crossover (SCO) - the largest such discrete SCO material reported. Now included in this family are the Se and BH{sub 3} analogues, Fe(NCSe)-nano and Fe(NCBH{sub 3})-nano, which show increased thermal transition temperatures (T{sub 1/2} = 124 K, 162 and 173 K). This variation in transition temperature over the series S < Se < BH{sub 3} results in diverse photomagnetic properties, such that the light-induced excited spin state trapping (LIESST) effect is exhibited to varying degrees and at different temperatures by the S, Se and BH{sub 3} analogues.

Duriska, Martin B.; Neville, Suzanne M.; Moubaraki, Boujemaa; Murray, Keith S.; Balde, Chrif; Ltard, Jean-Franois; Kepert, Cameron J.; Batten, Stuart R. (Sydney); (Monash); (CNRS-LSP)

2012-08-20T23:59:59.000Z

90

Complete genome sequence of the marine, cellulose and xylan degrading bacterium Glaciecola sp. 4H-3-7+YE-5  

DOE Green Energy (OSTI)

Glaciecola sp. 4H-3-7+YE-5 was isolated from deep sea sediments at Suruga Bay in Japan and is capable of efficiently hydrolyzing cellulose and xylan. The complete genome sequence of Glaciecola sp. 4H-3-7+YE-5 revealed several genes encoding putatively novel glycoside hydrolases associated with plant biomass degradation.

Klippel, Dr Barbara [Technische Universitat Hamburg-Harburg (Hamburg University of Technology); Bruce, David [Los Alamos National Laboratory (LANL); Davenport, Karen W. [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Han, Shunsheng [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Wiebusch, Sigrid [Technische Universitat Hamburg-Harburg (Hamburg University of Technology); Basner, Alexander [Technische Universitat Hamburg-Harburg (Hamburg University of Technology); Abe, Fumiyoshi [Japan Agency for Marine-Earth Science and Technology (JAMSTEC); Horikoshi, Koki [Japan Agency for Marine-Earth Science and Technology (JAMSTEC); Antranikian, Garabed [Technische Universitat Hamburg-Harburg (Hamburg University of Technology)

2011-01-01T23:59:59.000Z

91

Bioprospecting metagenomes: Glycosyl hydrolases for converting biomass  

DOE Green Energy (OSTI)

Throughout immeasurable time, microorganisms evolved and accumulated remarkable physiological and functional heterogeneity, and now constitute the major reserve for genetic diversity on earth. Using metagenomics, namely genetic material recovered directly from environmental samples, this biogenetic diversification can be accessed without the need to cultivate cells. Accordingly, microbial communities and their metagenomes, isolated from biotopes with high turnover rates of recalcitrant biomass, such as lignocellulosic plant cell walls, have become a major resource for bioprospecting; furthermore, this material is a major asset in the search for new biocatalytics (enzymes) for various industrial processes, including the production of biofuels from plant feedstocks. However, despite the contributions from metagenomics technologies consequent upon the discovery of novel enzymes, this relatively new enterprise requires major improvements. In this review, we compare function-based metagenome screening and sequence-based metagenome data mining, discussing the advantages and limitations of both methods. We also describe the unusual enzymes discovered via metagenomics approaches, and discuss the future prospects for metagenome technologies.

Li, L.; van der Lelie, D.; McCorkle, S. R.; Monchy, S.; Taghavi, S.

2009-05-18T23:59:59.000Z

92

Monitoring conservative retrofits in single family buildings  

SciTech Connect

This study has provided detailed before-and-after information on the ambient and comfort conditions in nine single family buildings, and on the energy consumption of those buildings, for one or more energy conservation retrofits. The data were recorded in such a manner that as well as being able to determine the savings from the retrofits and the influence these retrofits have on the comfort conditions of the residence, the effects of the retrofits on time-of-day usage are also determinable. The following are included in appendices: a table of participant's names, site addresses and retrofit; significant dates and appropriate comments; a day of data and an annotated data set; pre-retrofit and post-retrofit audit data sheets; and usage history.

Richardson, C.S.

1992-12-06T23:59:59.000Z

93

A Nuclear Family: Y-12 National Security Complex | National Nuclear  

National Nuclear Security Administration (NNSA)

Nuclear Family: Y-12 National Security Complex | National Nuclear Nuclear Family: Y-12 National Security Complex | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > A Nuclear Family: Y-12 National Security Complex A Nuclear Family: Y-12 National Security Complex Posted By Office of Public Affairs Nuclear family "A Nuclear Family: Y-12 National Security Complex" is a four episode

94

Searching for the fourth family quarks through anomalous decays  

Science Conference Proceedings (OSTI)

The flavor democracy hypothesis predicts the existence of the fourth standard model family. Because of the high masses of the fourth family quarks, their anomalous decays could be dominant if certain criteria are met. This will drastically change the search strategy at hadron colliders. We show that the fourth standard model family down quarks with masses up to 400-450 GeV can be observed (or excluded) via anomalous decays by Tevatron.

Sahin, M.; Sultansoy, S.; Turkoz, S. [TOBB University of Economics and Technology, Physics Division, Ankara (Turkey); TOBB University of Economics and Technology, Physics Division, Ankara, Turkey and Institute of Physics, National Academy of Sciences, Baku (Azerbaijan); Ankara University, Department of Physics, Ankara (Turkey)

2010-09-01T23:59:59.000Z

95

Application for State Land Use Lease: Commercial/Multi-Family...  

Open Energy Info (EERE)

navigation, search GEOTHERMAL ENERGYGeothermal Home Reference Material: Application for State Land Use Lease: CommercialMulti-Family Details Activities (0) Areas (0) Regions (0)...

96

California Cuckoo Wasps in the Family Chrysididae (Hymenoptera)  

E-Print Network (OSTI)

values. University of California Publications in Entomology278-284. University of California Publications in EntomologyLateral view of body. California Cuckoo Wasps in the Family

Kimsey, Lynn S.

2006-01-01T23:59:59.000Z

97

NNSA Production Office tops Feds Feed Families campaign goal...  

NLE Websites -- All DOE Office Websites (Extended Search)

NNSA Production Office tops ... NNSA Production Office tops Feds Feed Families campaign goal Posted: September 16, 2013 - 9:45am Oak Ridge, Tenn. - Employees of the National...

98

SLAC National Accelerator Laboratory - SLAC Engages Youth, Families...  

NLE Websites -- All DOE Office Websites (Extended Search)

SLAC Engages Youth, Families at 2012 USA Science & Engineering Festival By Andy Freeberg May 7, 2012 With more than 150,000 participants, the second annual USA Science &...

99

Secretaries Chu and Donovan Sign Agreement to Help Working Families...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

under the DOE Weatherization Assistance Program, which is targeted to low-income households. By eliminating unnecessary red tape and helping more families weatherize their...

100

FAMILY ENVIRONMENT AND SUBSTANCE USE IN ADOLESCENT MALES.  

E-Print Network (OSTI)

??The purpose of this study was to examine the relationships between several aspects of family environment and adolescent substance use. Participants included 372 (M = (more)

Brechting, Emily H.

2004-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Blacks and the family cap: pregnancy, abortion, and spillovers  

Science Conference Proceedings (OSTI)

Mar 29, 2006 ... reduction in nonmarital birth rates, particularly among black women. This .... rates are 21% lower among blacks impacted by the family cap.

102

Women, Families, Work, and Poverty: A Cloudy Future  

E-Print Network (OSTI)

5. By "welfare," I mean the AFDC program. WOMEN, FAMILIES,with poverty, but the AFDC welfare population isbility, food stamps, and AFDC, also need adjustments to

Handler, Joel F.

1996-01-01T23:59:59.000Z

103

Austin Energy - Multi-Family Energy Efficiency Rebate Program...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

3 - 4bulb Hardwire Retrofits: 9 Fixture Replacement 9 - 12 High Performance Ballast: 6 - 11 Occupancy Sensors: 5 - 32 The Austin Energy Multi-Family Program provides...

104

Comparison of heat pump system and boiler plant for one-family house : Heat sources in one-family house.  

E-Print Network (OSTI)

??The aim of this work is to look through, compare and choose the cheapest heat source for typical new Finnish one-family house. We will speak (more)

Kaydalova, Natalia

2010-01-01T23:59:59.000Z

105

Ubiquitous systems and the family: thoughts about the networked home  

Science Conference Proceedings (OSTI)

Developments in ubiquitous and pervasive computing herald a future in which computation is embedded into our daily lives. Such a vision raises important questions about how people, especially families, will be able to engage with and trust such systems ... Keywords: privacy, social interaction, the family, trust, ubiquitous computing

Linda Little; Elizabeth Sillence; Pam Briggs

2009-07-01T23:59:59.000Z

106

Can a Family Farm Benefit from Section 9006?  

DOE Green Energy (OSTI)

Section 9006 of the 2002 U.S. Farm Bill provides grants and guaranteed loans for rural renewable energy and energy efficiency projects. This fact sheet describes how the Tjaden family of Iowa pursued Section 9006 funding in pursuit of a wind turbine for their family farm.

Not Available

2005-05-01T23:59:59.000Z

107

Expenditures on Children by Families | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

Expenditures on Children by Families Expenditures on Children by Families Agriculture Community Menu DATA APPS EVENTS DEVELOPER STATISTICS COLLABORATE ABOUT Agriculture You are here Data.gov » Communities » Agriculture » Data Expenditures on Children by Families Dataset Summary Description This dataset provides expenditures on Children by Families provides estimates of the cost of raising children from birth through age 17 for major budgetary components. Tags {children,families,expenditures,cost,budget,household,income,single-parent,husband-wife} Dataset Ratings Overall 0 No votes yet Data Utility 0 No votes yet Usefulness 0 No votes yet Ease of Access 0 No votes yet Dataset Additional Information Last Updated 2012 Publisher Food and Nutrition Service, Department of Agriculture Contact Name Contact Email Mark.Lino@cnpp.usda.gov

108

Secretaries Chu and Donovan Sign Agreement to Help Working Families  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Donovan Sign Agreement to Help Working Families Donovan Sign Agreement to Help Working Families Weatherize Their Homes Secretaries Chu and Donovan Sign Agreement to Help Working Families Weatherize Their Homes May 6, 2009 - 12:00am Addthis WASHINGTON - U.S. Department of Housing and Urban Development (HUD) Secretary Shaun Donovan and U.S. Department of Energy (DOE) Secretary Steven Chu today announced an important step forward in the partnership between HUD and DOE to streamline and better coordinate federal weatherization programs. These efforts will make it easier for low-income families to weatherize their homes, saving money for working families and creating tens of thousands of new green jobs. Today, the Secretaries signed a Memorandum of Understanding (MOU) to coordinate energy retrofit programs in the American Recovery and

109

Seattle City Light - Multi-Family Residential Energy Efficiency Rebate  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Seattle City Light - Multi-Family Residential Energy Efficiency Seattle City Light - Multi-Family Residential Energy Efficiency Rebate Program Seattle City Light - Multi-Family Residential Energy Efficiency Rebate Program < Back Eligibility Multi-Family Residential Savings Category Home Weatherization Commercial Weatherization Appliances & Electronics Commercial Lighting Lighting Windows, Doors, & Skylights Program Info State District of Columbia Program Type Utility Rebate Program Rebate Amount Lighting: 85% discount on installation costs Insulation: 50% discount on installation costs Window Replacement: $3 - $5/sq. ft. Provider Seattle City Light Seattle City Light provides incentives for its multi-family housing customers to increase their energy efficiency. Rebates are offered for common area lighting and weatherization measures including the installation

110

Feds Feed Families campaign underway | National Nuclear Security  

National Nuclear Security Administration (NNSA)

Feds Feed Families campaign underway | National Nuclear Security Feds Feed Families campaign underway | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > Feds Feed Families campaign underway Feds Feed Families campaign underway Posted By Office of Public Affairs An event featuring live music, a dunk tank and a barbeque was held yesterday as part of the 2012 Feds Feed Families campaign. The DOE-wide

111

'Can Do' Attitude Brings EM Family Together | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

'Can Do' Attitude Brings EM Family Together 'Can Do' Attitude Brings EM Family Together 'Can Do' Attitude Brings EM Family Together December 11, 2013 - 12:00pm Addthis Jim Kopotic and his daughter Lauren pause for a photo after a race that raised money for cancer research and treatment. Jim Kopotic and his daughter Lauren pause for a photo after a race that raised money for cancer research and treatment. Jim (far right) talks with workers at the K-25 demolition site. Jim (far right) talks with workers at the K-25 demolition site. Jim’s influence and inspiration led friends and family to form the largest team at this year’s regional event. Jim's influence and inspiration led friends and family to form the largest team at this year's regional event. Jim Kopotic and his daughter Lauren pause for a photo after a race that raised money for cancer research and treatment.

112

Savannah River Nuclear Solutions awards family scholarships | National  

National Nuclear Security Administration (NNSA)

Savannah River Nuclear Solutions awards family scholarships | National Savannah River Nuclear Solutions awards family scholarships | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > Savannah River Nuclear Solutions awards family scholarships Savannah River Nuclear Solutions awards family scholarships Posted By Office of Public Affairs Savannah River Nuclear Solutions awards family scholarships

113

Sensemaking in a High-Risk Lifestyle: The Relationship Between Work and Family for Public Safety Families  

E-Print Network (OSTI)

Past research concerning work and family has largely been from traditional, white-collar settings and has only taken into consideration the perceptions of the employees' experiences with regard to the relationship between work and family. However, there is no doubt that employees' in non-traditional settings, particularly those employed in public safety professions (i.e. police and fire) experience the relationship between work and family differently than those in white-collar settings, especially since they put their lives on the line daily for the protection and betterment of the community, society and even the world. In addition, the experiences and perceptions of work and family will undoubtedly be different for the family members (i.e. children and spouses) of those employed in such "life-threatening" professions. This study sought to understand how public safety employees, as well as their families, make sense out of the relationship between work and family by first examining what metaphors they employ to articulate the relationship between work and family. In addition, this study sought to examine if male versus female public safety employees experience the relationship between work and family in similar or different ways, as well as if police officers and fire fighters experience the relationship similarly or differently. Using qualitative methods, the findings indicate that public safety employees and their families articulate and make sense of the relationship between work and family in both similar and different ways. Contrary to previous work-family research, dominant metaphors and constructs such as balance, conflict, segmentation, etc. did not appear at all within this study. Instead, participants likened the relationship between work and family to competition, nature, organism, change, integration, opposition, ambiguity, and destruction. Public safety employees and their families also experienced and made sense of the relationship between work and family through humor, emotion management, fear and risk assessment. Findings also indicate that both male and female public safety employees internalize risk in much the same way, as well as agree that parenthood in general, is devalued in the public safety profession. With regard to differences, findings indicate that females have a harder time negotiating a healthy relationship between work and family, have their competency levels always questioned by family or co-workers, and use different language and rhetoric from males when talking about work and family. Finally, results show that police officers and fire fighters make sense of work and family in much the same way with regard to "dirty work" and communication rules but differ in terms of coping mechanisms and job satisfaction. This study suggests a number of implications for both theory and practice. The findings also point to many necessary areas of future research which could further our understanding of the relationship between work and family, not only in professions characterized by high-risk, non-standard hours and stress, but also in standard white-collar professions as well.

Bochantin, Jaime Elizabeth

2010-08-01T23:59:59.000Z

114

Caldicellulosiruptor Core and Pangenomes Reveal Determinants for  

Science Conference Proceedings (OSTI)

Extremely thermophilic bacteria of the genus Caldicellulosiruptor utilize carbohydrate components of plant cell walls, including cellulose and hemicellulose, facilitated by a diverse set of glycoside hydrolases (GHs). From a biofuel perspective, this capability is crucial for deconstruction of plant biomass into fermentable sugars. While all species from the genus grow on xylan and acidpretreated switchgrass, growth on crystalline cellulose is variable. The basis for this variability was examined using microbiological, genomic, and proteomic analyses of eight globally diverse Caldicellulosiruptor species. The open Caldicellulosiruptor pangenome (4,009 open reading frames [ORFs]) encodes 106 GHs, representing 43 GH families, but only 26 GHs from 17 families are included in the core (noncellulosic) genome (1,543 ORFs). Differentiating the strongly cellulolytic Caldicellulosiruptor species from the others is a specific genomic locus that encodes multidomain cellulases from GH families 9 and 48, which are associated with cellulose-binding modules. This locus also encodes a novel adhesin associated with type IV pili, which was identified in the exoproteome bound to crystalline cellulose. Taking into account the core genomes, pangenomes, and individual genomes, the ancestral Caldicellulosiruptor was likely cellulolytic and evolved, in some cases, into species that lost the ability to degrade crystalline cellulose while maintaining the capacity to hydrolyze amorphous cellulose and hemicellulose.

Blumer-Schuette, Sara E. [North Carolina State University; Giannone, Richard J [ORNL; Zurawski, Jeffrey V [North Carolina State University; Ozdemir, Inci [North Carolina State University; Ma, Qin [University of Georgia, Athens, GA; Yin, Yanbin [University of Georgia, Athens, GA; Xu, Ying [University of Georgia, Athens, GA; Kataeva, Irena [University of Georgia, Athens, GA; Poole, Farris [University of Georgia, Athens, GA; Adams, Michael W. W. [University of Georgia, Athens, GA; Hamilton-Brehm, Scott [ORNL; Elkins, James G [ORNL; Larimer, Frank W [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Cottingham, Robert W [ORNL; Hettich, Robert {Bob} L [ORNL; Kelly, Robert M [North Carolina State University

2012-01-01T23:59:59.000Z

115

A Collisional Family in the Classical Kuiper Belt  

E-Print Network (OSTI)

The dynamical evolution of Classical Kuiper Belt Objects (CKBOs) divides into two parts, according to the secular theory of test particle orbits. The first part is a forced oscillation driven by the planets, while the second part is a free oscillation whose amplitude is determined by the initial orbit of the test particle. We extract the free orbital inclinations and free orbital eccentricities from the osculating elements of 125 known CKBOs. The free inclinations of 32 CKBOs strongly cluster about 2 degrees at orbital semi-major axes between 44 and 45 AU. We propose that these objects comprise a collisional family, the first so identified in the Kuiper Belt. Members of this family are plausibly the fragments of an ancient parent body having a minimum diameter of \\~800 km. This body was disrupted upon colliding with a comparably sized object, and generated ejecta having similar free inclinations. Our candidate family is dynamically akin to a sub-family of Koronis asteroids located at semi-major axes less than 2.91 AU; both families exhibit a wider range in free eccentricity than in free inclination, implying that the relative velocity between parent and projectile prior to impact lay mostly in the invariable plane of the solar system. We urge more discoveries of new CKBOs to test the reality of our candidate family and physical studies of candidate family members to probe the heretofore unseen interior of a massive, primitive planetesimal.

E. I. Chiang

2002-05-16T23:59:59.000Z

116

Puget Sound Energy - Multi-Family Efficiency Programs | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

You are here You are here Home » Puget Sound Energy - Multi-Family Efficiency Programs Puget Sound Energy - Multi-Family Efficiency Programs < Back Eligibility Construction Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Home Weatherization Commercial Weatherization Appliances & Electronics Manufacturing Heat Pumps Commercial Lighting Lighting Water Heating Windows, Doors, & Skylights Solar Swimming Pool Heaters Program Info State District of Columbia Program Type Utility Rebate Program Rebate Amount Multi-Family Retrofit CFLs: $20/fixture or FREE LEDs: $20- $30 Windows/Sliding Glass Doors: $6 - $8/sq. ft. Insulation: $0.75/sq. ft. In-Unit Water Heater: $50/unit Clothes Washer: $50 - $100 In-Unit Refrigerator: $20 Solar Pool Heater: Not Specified

117

The DOE Feeds Families Festival | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

The DOE Feeds Families Festival The DOE Feeds Families Festival The DOE Feeds Families Festival July 27, 2010 - 5:50pm Addthis Andy Oare Andy Oare Former New Media Strategist, Office of Public Affairs Today, Department of Energy employees took a moment to give back to the Washington, D.C. community by holding our first "DOE Feeds Families Festival" outside of the Forrestal Building. Highlights from the event included a dunk tank for some of DOE's top officials (featuring NNSA Administrator Thomas D'Agostino, DOE Chief Human Capital Officer Michael Kane, and Senior Advisor Matt Rogers), and OPM Director, John Berry, to name a few. Additionally, Deputy Secretary Daniel Poneman and his band "Yellowcake" graced the stage for a rock concert, featuring covers of popular classic rock songs.

118

A Family-Friendly Workplace | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Services » New Employee Orientation » A Family-Friendly Workplace Services » New Employee Orientation » A Family-Friendly Workplace A Family-Friendly Workplace The Department of Energy (DOE) takes its commitment to recruiting, retaining, and motivating a highly skilled, diverse, and productive workforce very seriously. Our objective is to be the employer of choice in the public sector. The U.S. Department of Energy is the Top-Ranked Cabinet-Level Agency for employee satisfaction and engagement according to the 2005 Best Places to Work in the Federal Government Rankings. Below is a summary of additional employment benefits and family-friendly programs. Annual and Sick Leave As a new Federal employee, you will earn both Annual (vacation) leave and Sick leave. Employees earn four hours of Sick leave each pay period during

119

SCE - Multi-Family Residential Energy Efficiency Programs | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Multi-Family Residential Energy Efficiency Programs Multi-Family Residential Energy Efficiency Programs SCE - Multi-Family Residential Energy Efficiency Programs < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Cooling Appliances & Electronics Heat Pumps Commercial Lighting Lighting Manufacturing Water Heating Home Weatherization Windows, Doors, & Skylights Other Program Info Funding Source System Benefits Charge Start Date 1/1/2012 Expiration Date 12/31/2012 State California Program Type Utility Rebate Program Rebate Amount LED Pool/Spa Lighting: $75 - $100/unit Pool Pumps: $100 Energy Star Ceiling Fan (with Energy Star CFLs): $20/unit High Efficiency Clothes Washers: $50 - $100/unit Energy Star Refrigerators: $50/unit Dual Pane Windows: $0.75/sq. ft.

120

NNSA Production Office tops Feds Feed Families campaign goal | National  

NLE Websites -- All DOE Office Websites (Extended Search)

Production Office tops Feds Feed Families campaign goal | National Production Office tops Feds Feed Families campaign goal | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > NNSA Production Office tops Feds Feed Families ... NNSA Production Office tops Feds Feed Families campaign goal Posted By Office of Public Affairs Employees of the NNSA Production Office (NPO) have donated 17,348 pounds of

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Austin Energy - Multi-Family Energy Efficiency Rebate Program | Department  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Austin Energy - Multi-Family Energy Efficiency Rebate Program Austin Energy - Multi-Family Energy Efficiency Rebate Program Austin Energy - Multi-Family Energy Efficiency Rebate Program < Back Eligibility Multi-Family Residential Savings Category Home Weatherization Commercial Weatherization Heating & Cooling Commercial Heating & Cooling Cooling Sealing Your Home Ventilation Heat Pumps Appliances & Electronics Commercial Lighting Lighting Insulation Design & Remodeling Windows, Doors, & Skylights Maximum Rebate $200,000 Program Info State Texas Program Type Utility Rebate Program Rebate Amount Split System Air Conditioning: $200 - $550 Packaged Unit Air Conditioning: $300 - $500 Split System Heat Pumps: $250 - $600 Packaged Unit Heat Pumps: $350 - $550 Solar Screens/Solar Film: $1.00 - $1.25/sq. ft. Low E Window Replacement: $2.00/sq. ft.

122

Burlington Electric Department - Multi-Family Rental Energy Efficiency  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Multi-Family Rental Energy Multi-Family Rental Energy Efficiency Rebate Program Burlington Electric Department - Multi-Family Rental Energy Efficiency Rebate Program < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Appliances & Electronics Commercial Lighting Lighting Maximum Rebate Rebates totaling over 2,500 must be pre-approved by BED More than 15 free CFL's per apartment or 100 free CFL's per building requires pre-approval Program Info State Vermont Program Type Utility Rebate Program Rebate Amount CFLs: Free Ventilation Systems: $110/unit Boilers: $2/MBh Furnaces: $2/MBh Electronically Commutated Motor: $100 Refrigerators: $150/unit Lighting: In-store discounts Provider Burlington Electric Department Burlington Electric Department offers an innovative rebate program geared

123

West Virginian No Longer Worried About Family's Safety | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Virginian No Longer Worried About Family's Safety Virginian No Longer Worried About Family's Safety West Virginian No Longer Worried About Family's Safety April 2, 2010 - 2:29pm Addthis Joshua DeLung When the mountain winters hit for the last 12 years, Michael Shepard and his family had only a wood stove in their house for heat, plus a few kerosene heaters when the stove didn't do the trick. Michael's diagnosis as a diabetic, coupled with a shoulder injury, left him disabled, resulting in his early retirement. Relying on a wood stove with a serious shoulder injury meant getting heat in Michael's Bluefield, W.Va., home was a struggle. "It was cold, just using wood heat, and my wife had to carry it in for me," he says. After applying for and receiving weatherization assistance from Community Action of South Eastern West Virginia last year, though, his lifestyle at

124

LIPABASE: a database for |true| lipase family enzymes  

Science Conference Proceedings (OSTI)

Lipase enzymes play an important role in lipid metabolism and are produced by a variety of species. Compared with animal, bacterial and fungal, little is known about plant lipases. Although lipases belong to many different protein families, they have ...

Abdelmonaem Messaoudi; Hatem Belguith; Imen Ghram; Jeannette Ben Hamida

2011-11-01T23:59:59.000Z

125

Pocket protein family function in mesenchymal tissue development and tumorigenesis  

E-Print Network (OSTI)

pRB is a member of the pocket protein family, which includes the closely related proteins p107 and p130. The pocket proteins are critical regulators of the cell cycle and function to restrain proliferation by controlling ...

Landman, Allison Simone

2009-01-01T23:59:59.000Z

126

Data complexity in the ?L family of description logics  

Science Conference Proceedings (OSTI)

We study the data complexity of instance checking and conjunctive query answering in the EL family of description logics, with a particular emphasis on the boundary of tractability. We identify a large number of intractable extensions of EL, but also ...

Adila Krisnadhi; Carsten Lutz

2007-10-01T23:59:59.000Z

127

North Shore Gas Single Family Direct Install (Illinois)  

Energy.gov (U.S. Department of Energy (DOE))

Owners of single-family homes, condos, townhomes and two-flats may be eligible for a free installation of new programmable thermostats, pipe insulation, showerheads, and faucet aerators through...

128

Ameren Illinois (Electric) - Multi-Family Properties Energy Efficiency  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Ameren Illinois (Electric) - Multi-Family Properties Energy Ameren Illinois (Electric) - Multi-Family Properties Energy Efficiency Rebate Program Ameren Illinois (Electric) - Multi-Family Properties Energy Efficiency Rebate Program < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Cooling Home Weatherization Commercial Weatherization Sealing Your Home Ventilation Manufacturing Heat Pumps Appliances & Electronics Commercial Lighting Lighting Maximum Rebate See rebate amounts listed above Program Info State Illinois Program Type Utility Rebate Program Rebate Amount '''Common Area Efficiency''' CFL's: $1.50 Modular CFL's: $23-$26 T8 Lamps: $7-$12, depending on ballast and wattage Occupancy Sensors: $25 LED Exit Sign: $22 In-Unit Efficiency Installations: CFLs, pipe insulation and water savings

129

California Solar Initiative - Multi-Family Affordable Solar Housing (MASH)  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

California Solar Initiative - Multi-Family Affordable Solar Housing California Solar Initiative - Multi-Family Affordable Solar Housing (MASH) Program California Solar Initiative - Multi-Family Affordable Solar Housing (MASH) Program < Back Eligibility Low-Income Residential Multi-Family Residential Savings Category Solar Buying & Making Electricity Program Info State California Program Type State Rebate Program Rebate Amount Track 1: Fully Subscribed Track 2: Closed '''''Track 2 was closed in 2011. Track 1 incentives have been fully subscribed for all three program administrators and waitlists have been established. Contact the appropriate program administrator for up to date information on the status of Track 1. ''''' The California Solar Initiative (CSI) provides financial incentives to customers in investor-owned utility (IOU) territories of Pacific Gas and

130

NNSA Administrator Kicks Off DOE's 'Feds Feed Families' Campaign |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Kicks Off DOE's 'Feds Feed Families' Campaign Kicks Off DOE's 'Feds Feed Families' Campaign NNSA Administrator Kicks Off DOE's 'Feds Feed Families' Campaign June 16, 2011 - 12:00am Addthis WASHINGTON, D.C. - National Nuclear Security Administration (NNSA) Administrator Thomas P. D'Agostino today helped the Department of Energy (DOE) kick off its third annual "Feds Feed Families" campaign to assist local food banks in replenishing supplies during the summer months. DOE and NNSA, in partnership with the Office of Personnel Management (OPM) and other Federal agencies are participating in the nationwide effort to raise 2 million pounds of non-perishable food items for food banks across the country. Administrator D'Agostino and DOE's Chief Human Capital Officer Mike Kane once again serve as DOE campaign co-chairs.

131

Y-12 and East TN Public Broadcasting System ? A Nuclear Family...  

NLE Websites -- All DOE Office Websites (Extended Search)

East TN Public Broadcasting System - A Nuclear Family Video Miniseries The fourth and final episode of A Nuclear Family: Y-12 National Security Complex documentary film miniseries...

132

Suite of Activity-Based Probes for Cellulose-Degrading Enzymes  

Science Conference Proceedings (OSTI)

Microbial glycoside hydrolases play a dominant role in the biochemical conversion of cellulosic biomass to high-value biofuels. Anaerobic cellulolytic bacteria are capable of producing multicomplex catalytic subunits containing cell-adherent cellulases, hemicellulases, xylanases, and other glycoside hydrolases to facilitate the degradation of highly recalcitrant cellulose and other related plant cell wall polysaccharides. Clostridium thermocellum is a cellulosome producing bacterium that couples rapid reproduction rates to highly efficient degradation of crystalline cellulose. Herein, we have developed and applied a suite of difluoromethylphenyl aglycone, N-halogenated glycosylamine, and 2-deoxy-2-fluoroglycoside activity-based protein profiling (ABPP) probes to the direct labeling of the C. thermocellum cellulosomal secretome. These activity-based probes (ABPs) were synthesized with alkynes to harness the utility and multimodal possibilities of click chemistry, and to increase enzyme active site inclusion for LC-MS analysis. We directly analyzed ABP-labeled and unlabeled global MS data, revealing ABP selectivity for glycoside hydrolase (GH) enzymes in addition to a large collection of integral cellulosome-containing proteins. By identifying reactivity and selectivity profiles for each ABP, we demonstrate our ability to widely profile the functional cellulose degrading machinery of the bacterium. Derivatization of the ABPs, including reactive groups, acetylation of the glycoside binding groups, and mono- and disaccharide binding groups, resulted in considerable variability in protein labeling. Our probe suite is applicable to aerobic and anaerobic cellulose degrading systems, and facilitates a greater understanding of the organismal role associated within biofuel development.

Chauvigne-Hines, Lacie M.; Anderson, Lindsey N.; Weaver, Holly M.; Brown, Joseph N.; Koech, Phillip K.; Nicora, Carrie D.; Hofstad, Beth A.; Smith, Richard D.; Wilkins, Michael J.; Callister, Stephen J.; Wright, Aaron T.

2012-12-19T23:59:59.000Z

133

Apples and Other Fruits of the Rose Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Apples and Other Fruits of the Rose Family Apples and Other Fruits of the Rose Family Nature Bulletin No. 277-A October 7, 1967 Forest Preserve District of Cook County Richard B. Ogilvie, President Roland F. Eisenbeis, Supt. of Conservation APPLES AND OTHER FRUITS OF THE ROSE FAMILY Bread, meat and potatoes, with vegetables and green salads, may satisfy the inner man and keep us healthy but much of the fun of eating is provided by the rose family. The rose, itself, is the flower of chivalry and love but its relative, the apple, king of the fruits, has been cultivated since prehistoric times. The members of the Rose Family give us more pleasure than we get from any other group of plants. Apples, quinces, pears, peaches, plums, apricots, cherries, blackberries, raspberries and strawberries -- whether wild or cultivated -- are all relatives of the roses. Their fruits, raw or cooked, give us desserts, pleasant drinks, and many a between-meal delicacy. Typically, they have showy flowers with five equal petals arranged around a central cup bearing one or more fruit-forming pistils and a large number of pollen-bearing stamens. Their leaves are placed alternately on the twigs or stalks -- some of them simple leaves; some of them compound leaves divided into three, five or more leaflets like the rose itself. Some are trees, some are shrubs, and some are vining herbs.

134

Characterization of a New Family of Metal Transporters  

Science Conference Proceedings (OSTI)

Metal ions are critical nutrients, yet overaccumulation of these same metals can also be toxic. To maintain appropriate intracellular levels, cells require specific metal uptake systems that are subject to precise homeostatic regulation. The long-range goal of our research is to define the molecular mechanism(s) and regulation of metal ion uptake in eukaryotic cells. Integrating genetic, molecular biological and biochemical approaches, we have examined these processes in the yeast Saccharomyces cerevisiae and the plant Arabidopsis thaliana. Both are proven model systems for studying fundamental cellular processes. Our work has focused on the ZIP family of metal transporters which we identified; this family has representatives in bacteria, fungi, plants and animals. IRT, one of the founding members of the ZIP family, is an essential cation transporter that is expressed in the epidermal cells of iron deficient plant roots and is responsible for uptake of iron from the soil. We now know that there are 15 ZIP genes in the Arabidopsis and the similarities among their encoded gene products. The ZIP family members display different substrate specificities for metals and different tissue distributions in Arabidopsis. Moreover, the family members respond differentially to metal deficiencies. For example, IRT1, ZIP6 and ZIP9 mRNA are expressed mainly in the roots of iron deficient plants whereas ZIP4 responds to both iron and zinc deficiency. Work in both yeast and Arabidopsis has addressed substrate specificity as well as how these transporters are regulated in response to metal availability

Mary Lou Geurinot; David Eide

2002-04-29T23:59:59.000Z

135

A Collisional Family in the Classical Kuiper Belt  

E-Print Network (OSTI)

The dynamical evolution of Classical Kuiper Belt Objects (CKBOs) divides into two parts, according to the secular theory of test particle orbits. The first part is a forced oscillation driven by the planets, while the second part is a free oscillation whose amplitude is determined by the initial orbit of the test particle. We extract the free orbital inclinations and free orbital eccentricities from the osculating elements of 125 known CKBOs. The free inclinations of 32 CKBOs strongly cluster about 2 degrees at orbital semi-major axes between 44 and 45 AU. We propose that these objects comprise a collisional family, the first so identified in the Kuiper Belt. Members of this family are plausibly the fragments of an ancient parent body having a minimum diameter of \\~800 km. This body was disrupted upon colliding with a comparably sized object, and generated ejecta having similar free inclinations. Our candidate family is dynamically akin to the Koronis family of asteroids; both families exhibit a wider range i...

Chiang, E I

2002-01-01T23:59:59.000Z

136

An Update on Feds Feeds Families | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

An Update on Feds Feeds Families An Update on Feds Feeds Families An Update on Feds Feeds Families November 19, 2012 - 5:32pm Addthis As a result of DOE employee efforts, the Energy Department received an award from OPM for donating the highest amount of non-perishable food items within the “small agency” category of Federal agencies participating in this year’s Campaign. | Photo courtesy of OPM. As a result of DOE employee efforts, the Energy Department received an award from OPM for donating the highest amount of non-perishable food items within the "small agency" category of Federal agencies participating in this year's Campaign. | Photo courtesy of OPM. April Saylor April Saylor Former Digital Outreach Strategist, Office of Public Affairs What does this mean for me?

137

California Solar Initiative - Single-Family Affordable Solar Housing (SASH)  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

California Solar Initiative - Single-Family Affordable Solar California Solar Initiative - Single-Family Affordable Solar Housing (SASH) Program California Solar Initiative - Single-Family Affordable Solar Housing (SASH) Program < Back Eligibility Low-Income Residential Savings Category Solar Buying & Making Electricity Maximum Rebate 10,000 for fully subsidized systems No maximum stated for partially subsidized systems Program Info Start Date 7/1/2009 Expiration Date 12/31/2015 State California Program Type State Rebate Program Rebate Amount Varies depending on participant's income level and California Alternate Rates for Energy (CARE) program eligibility. (see below) Provider GRID Alternatives The California Solar Initiative (CSI) provides financial incentives for installing solar technologies through a variety of smaller sub-programs. Of

138

Secretaries Donovan and Chu Announce Partnership to Help Working Families  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Secretaries Donovan and Chu Announce Partnership to Help Working Secretaries Donovan and Chu Announce Partnership to Help Working Families Weatherize Their Homes Secretaries Donovan and Chu Announce Partnership to Help Working Families Weatherize Their Homes February 27, 2009 - 12:00am Addthis Philadelphia, PA - U.S. Department of Housing and Urban Development (HUD) Secretary Shaun Donovan and U.S. Department of Energy (DOE) Secretary Steven Chu today announced a major partnership between HUD and DOE that will streamline and better coordinate federal weatherization efforts to make it much easier for families to weatherize their homes and spur a new home energy efficiency industry that could create tens of thousands of jobs. Vice President Joe Biden, joined by Secretary Donovan and Secretary Chu, praised the announcement during his remarks at a meeting of the White House

139

Weatherization Saves Families Energy and Money | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Weatherization Saves Families Energy and Money Weatherization Saves Families Energy and Money Weatherization Saves Families Energy and Money October 28, 2013 - 12:50pm Addthis William Stewart, with Veterans Green Jobs, blows cellulose insulation in the interior walls of a Lakewood, Colorado, home. This home is part of the Energy Department's Weatherization Assistance Program that supports energy efficiency upgrades to low-income homes in Denver. | Photo courtesy of Dennis Schroeder, NREL. William Stewart, with Veterans Green Jobs, blows cellulose insulation in the interior walls of a Lakewood, Colorado, home. This home is part of the Energy Department's Weatherization Assistance Program that supports energy efficiency upgrades to low-income homes in Denver. | Photo courtesy of Dennis Schroeder, NREL.

140

Neutron Sciences - Neutron Scattering Dynamics in Polymer Family  

NLE Websites -- All DOE Office Websites (Extended Search)

Neutron scattering characterizes dynamics in polymer family Neutron scattering characterizes dynamics in polymer family Research Contact: Christine Gerstl December 2012, Written by Agatha Bardoel Understanding the interplay between structure and dynamics is the key to obtaining tailor-made materials. In the last few years, a large effort has been devoted to characterizing and relating the structure and dynamic properties in families of polymers with alkyl side groups. Now researchers have used quasielastic neutron scattering to investigate the hydrogen dynamics in poly(alkylene oxide)s with different side-chain lengths at temperatures below, as well as above, the glass transition. The combination of techniques and instruments used is bringing a more complete understanding of multiple contributions to system dynamics. The results

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Weatherization Saves Families Energy and Money | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Weatherization Saves Families Energy and Money Weatherization Saves Families Energy and Money Weatherization Saves Families Energy and Money October 28, 2013 - 12:50pm Addthis William Stewart, with Veterans Green Jobs, blows cellulose insulation in the interior walls of a Lakewood, Colorado, home. This home is part of the Energy Department's Weatherization Assistance Program that supports energy efficiency upgrades to low-income homes in Denver. | Photo courtesy of Dennis Schroeder, NREL. William Stewart, with Veterans Green Jobs, blows cellulose insulation in the interior walls of a Lakewood, Colorado, home. This home is part of the Energy Department's Weatherization Assistance Program that supports energy efficiency upgrades to low-income homes in Denver. | Photo courtesy of Dennis Schroeder, NREL.

142

Teaching Humanities in Medicine: The University of Massachusetts Family Medicine Residency Program Experience  

E-Print Network (OSTI)

on Family Medicine essays, creative prose, or poetry, the Creative Writing Award stories, poems, essays; second,

Silk, Hugh; Shields, Sara

2012-01-01T23:59:59.000Z

143

Tangencies between families of disjoint regions in the plane  

Science Conference Proceedings (OSTI)

Let C be a family of n convex bodies in the plane, which can be decomposed into k subfamilies of pairwise disjoint sets. It is shown that the number of tangencies between the members of C is at most O(kn), and that this bound cannot be improved. If we ... Keywords: Arrangements, Convexity, Tangencies

Jnos Pach; Andrew Suk; Miroslav Treml

2012-04-01T23:59:59.000Z

144

A polynomial family of PD-type Cartesian controllers  

Science Conference Proceedings (OSTI)

This paper analyzes the position control problem of robot manipulators on Cartesian space. The main objective of this paper is to propose a new controller family with gravity compensation and formal stability proof using Lyapunov's theory. We have used ... Keywords: Cartesian controller, DRILL-BOT, Jacobian transposed controller, Lyapunov function, artificial potential energy, energy shaping, global asymptotical stability

P. Snchez-Snchez; F. Reyes-Corts

2008-03-01T23:59:59.000Z

145

Sharing motion information with close family and friends  

Science Conference Proceedings (OSTI)

We present the Motion Presence application, an augmented phone book style application that allows close friends and family to view each other's current motion status ("moving" or "not moving") on their mobile phones. We performed a two week long field ... Keywords: awareness, mobile presence, motion detection, privacy

Frank R. Bentley; Crysta J. Metcalf

2007-04-01T23:59:59.000Z

146

Characterization of a new family of metal transporters  

Science Conference Proceedings (OSTI)

Metal ions are critical nutrients, yet overaccumulation of these same metals can also be toxic. To maintain appropriate intracellular levels, cells require specific metal uptake systems that are subject to precise homeostatic regulation. The long-range goal of our research is to define the molecular mechanism(s) and regulation of metal ion uptake in eukaryotic cells. Integrating genetic, molecular biological and biochemical approaches, we have examined these processes in the yeast Saccharomyces cerevisiae and the plant Arabidopsis thaliana. Both are proven model systems for studying fundamental cellular processes. Our work has focused on the ZIP family of metal transporters which we identified; this family has representatives in bacteria, fungi,plants and animals. IRT1, one of the founding members of the ZIP family, is an essential cation transporter that is expressed in the epidermal cells of iron deficient plant roots and is responsible for uptake of iron from the soil. We now know that t here are 15 ZIP genes in the Arabidopsis genome which can be divided into four different classes, based on their intron/exon arrangements and the similarities among their encoded gene products. The ZIP family members display different substrate specificities for metals and different tissue distributions in Arabidopsis.Moreover, the family members respond differentially to metal deficiencies. For example, IRT1, ZIP6 and ZIP9 mRNA are expressed mainly in the roots of iron deficient plants whereas ZIP4 responds to both iron and zinc deficiency. Work in both yeast and Arabidopsis has addressed substrate specificity as well as how these transporters are regulated in response to metal availability. Our project was broken down into four specific aims. Significant progress was made on all four aims. I have listed the publications which have resulted under the relevant specific aim.

Guerinot, Mary L.; Dide, David

1999-12-31T23:59:59.000Z

147

Ames Laboratory scientists discover new family of quasicrystals | Argonne  

NLE Websites -- All DOE Office Websites (Extended Search)

Science Science Computing, Environment & Life Sciences Energy Engineering & Systems Analysis Photon Sciences Physical Sciences & Engineering Energy Frontier Research Centers Science Highlights Postdoctoral Researchers Ames Laboratory scientists discover new family of quasicrystals July 16, 2013 Tweet EmailPrint Scientists at the U.S. Department of Energy's (DOE) Ames Laboratory have discovered a new family of rare-earth quasicrystals using an algorithm they developed to help pinpoint them. Quasicrystalline materials may be found close to crystalline phases that contain similar atomic motifs, called crystalline approximants. And just like fishing experts know that casting a line in the right habitat hooks the big catch, the scientists used their knowledge to hone in on just the right spot for new quasicrystal materials

148

A New Family of Quasicrystals | Argonne National Laboratory  

NLE Websites -- All DOE Office Websites (Extended Search)

Science Science Computing, Environment & Life Sciences Energy Engineering & Systems Analysis Photon Sciences Physical Sciences & Engineering Energy Frontier Research Centers Science Highlights Postdoctoral Researchers A New Family of Quasicrystals August 19, 2013 Tweet EmailPrint Scientists from Ames Laboratory and Iowa State University carrying out research at the X-ray Science Division (XSD) beamline 6-ID-D at the U.S. Department of Energy Office of Science's Advanced Photon Source (APS) at Argonne characterized a new family of rare-earth quasicrystals. They are candidates for applications from hydrogen storage, to cylinder liners and piston coatings that could reduce air pollution and increase engine lifetimes, to coatings on metallic parts for bone repair and prostheses.

149

A New Family of Quasicrystals | Advanced Photon Source  

NLE Websites -- All DOE Office Websites (Extended Search)

Cool Muscles: Storing Elastic Energy for Flight Cool Muscles: Storing Elastic Energy for Flight A Further Understanding of Superconductivity New Family of Tiny Crystals Glow Bright in LED Lights How Serotonin Receptors Can Shape Drug Effects, from LSD to Migraine Medication X-rays Paint a Picture of Picasso's Pigments Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed A New Family of Quasicrystals JUNE 24, 2013 Bookmark and Share The pentagonal dodecahedral morphology of the icosahedral phase is shown with a millimeter scale; grains as large as 1.0 mm have been grown. Just as fishing experts know that casting a line in the right spot hooks the big catch, scientists from the U.S. Department of Energy Office of

150

Lepton Mixing Predictions from Delta(6n^2) Family Symmetry  

E-Print Network (OSTI)

We obtain predictions of lepton mixing parameters for direct models based on Delta(6n^2) family symmetry groups for arbitrarily large n in which the full Klein symmetry is identified as a subgroup of the family symmetry. After reviewing and developing the group theory associated with Delta(6n^2), we find many new candidates for large n able to yield reactor angle predictions within 3 sigma of recent global fits. We show that such Delta(6n^2) models with Majorana neutrinos predict trimaximal mixing with reactor angle theta_{13} fixed up to a discrete choice, an oscillation phase of either zero or pi and the atmospheric angle sum rules theta_{23}=45 degrees -/+ theta_{13}/sqrt{2}, respectively, which are consistent with recent global fits and will be tested in the near future.

King, Stephen F; Stuart, Alexander J

2013-01-01T23:59:59.000Z

151

Lepton Mixing Predictions from Delta(6n^2) Family Symmetry  

E-Print Network (OSTI)

We obtain predictions of lepton mixing parameters for direct models based on Delta(6n^2) family symmetry groups for arbitrarily large n in which the full Klein symmetry is identified as a subgroup of the family symmetry. After reviewing and developing the group theory associated with Delta(6n^2), we find many new candidates for large n able to yield reactor angle predictions within 3 sigma of recent global fits. We show that such Delta(6n^2) models with Majorana neutrinos predict trimaximal mixing with reactor angle theta_{13} fixed up to a discrete choice, an oscillation phase of either zero or pi and the atmospheric angle sum rules theta_{23}=45 degrees -/+ theta_{13}/sqrt{2}, respectively, which are consistent with recent global fits and will be tested in the near future.

Stephen F. King; Thomas Neder; Alexander J. Stuart

2013-05-14T23:59:59.000Z

152

EPRI Family of Low-Cost Multifunction Switchgear Systems  

Science Conference Proceedings (OSTI)

This project is to develop a family of low-cost solid-state switchgear systems for a range of distribution applications. These devices will be designed for use in switchgear replacements and for new installations. Additional benefits will come from other functionality (besides interrupting current) to be built into the switchgear systems. The switchgear systems will be useful in current distribution system infrastructure and, as a part of ADA, in migration to the distribution system of the future. The re...

2006-12-11T23:59:59.000Z

153

Delivering Energy Efficiency to Middle Income Single Family Households  

NLE Websites -- All DOE Office Websites (Extended Search)

Delivering Energy Efficiency to Middle Income Single Family Households Delivering Energy Efficiency to Middle Income Single Family Households Title Delivering Energy Efficiency to Middle Income Single Family Households Publication Type Report Year of Publication 2011 Authors Zimring, Mark, Merrian Borgeson, Ian M. Hoffman, Charles A. Goldman, Elizabeth Stuart, Annika Todd, and Megan A. Billingsley Pagination 102 Date Published 12/2011 Publisher LBNL City Berkeley Keywords electricity markets and policy group, energy analysis and environmental impacts department Abstract The question posed in this report is: How can programs motivate these middle income single family households to seek out more comprehensive energy upgrades, and empower them to do so? Research methods included interviews with more than 35 program administrators, policy makers, researchers, and other experts; case studies of programs, based on interviews with staff and a review of program materials and data; and analysis of relevant data sources and existing research on demographics, the financial status of Americans, and the characteristics of middle income American households. While there is no 'silver bullet' to help these households overcome the range of barriers they face, this report describes outreach strategies, innovative program designs, and financing tools that show promise in increasing the attractiveness and accessibility of energy efficiency for this group. These strategies and tools should be seen as models that are currently being honed to build our knowledge and capacity to deliver energy improvements to middle income households. However, the strategies described in this report are probably not sufficient, in the absence of robust policy frameworks, to deliver these improvements at scale. Instead, these strategies must be paired with enabling and complementary policies to reach their full potential.

154

Pressure Effects on Two Superconducting Iron-based Families  

SciTech Connect

Insight into the mechanism of high-temperature superconductivity can be gained by pressure-dependent studies of structural, thermodynamics and transport data. The role of pressure may be complicated by the level of hydrostaticity. High-pressure studies on two iron-based families of RFeAsO (R = rare-earth metals) and AFe{sub 2}As{sub 2} (A = alkaline-earth metals) are reviewed here.

Safa-Sefat, Athena [ORNL

2011-01-01T23:59:59.000Z

155

Geothermal System Saves Dollars, Makes Sense for Maryland Family |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

System Saves Dollars, Makes Sense for Maryland Family System Saves Dollars, Makes Sense for Maryland Family Geothermal System Saves Dollars, Makes Sense for Maryland Family April 16, 2010 - 5:15pm Addthis A 36-foot-tall drill was needed to install the geothermal system at the Gearon’s house in Derwood, MD. | Photo courtesy of Chris Gearon | A 36-foot-tall drill was needed to install the geothermal system at the Gearon's house in Derwood, MD. | Photo courtesy of Chris Gearon | Lindsay Gsell "At the end of the day, it cost us about the same as if we just replaced our furnace and AC with another furnace and AC, but the big difference is that we're not spending $3,000 on oil bills anymore." Chris Gearon, Derwood, MD resident who recently installed a geothermal system to heal and cool his home Chris Gearon's 24-year old oil furnace was tired. What happened if the

156

Characterization of a New Family of Metal Transport Proteins  

SciTech Connect

Soils at many DOE sites are contaminated with metals and radionuclides. Such soils obviously pose a risk to human and animal health. Unlike organic wastes, which can be metabolized, metals are immutable and cannot be degraded into harmless constituents. Phytoremediation, the use of plants to remove toxic materials from soil and water, may prove to be an environmentally friendly and cost effective solution for cleaning up metal contaminated sites. The success of phytoremediation will rely on the availability of plants that absorb, translocate, and tolerate the contaminating metals. However, before we can engineer such plants, we need more basic information on how plants acquire metals. An important long term goal of our research program is to understand how metals such as zinc, cadmium and iron are transported across membranes. Our research is focused on a new family of metal transporters, which we have identified through combined studies in the yeast Saccharomyces cerevisiae and in the model plant Arabidopsis thaliana. We have identified a family of 24 presumptive metal transport genes in a variety of organisms including yeast, trypanosomes, plants, nematodes, and humans. This family, which we have designated the ''ZIP'' genes, provides a rich source of material with which to undertake studies on metal transport in eukar

Guerinot, Mary Lou; Eide, David

1999-06-01T23:59:59.000Z

157

Rural Latino families in California are missing earned income tax benefits  

E-Print Network (OSTI)

for Central Cities and Remote- Rural Areas. Research brief.DC. www.urban.org/url.cfm? ID= Rural Families Speak Project.RESEARCH ARTICLE Rural Latino families in California are

Varcoe, Karen P.; Lees, Nancy B.; Lpez, Martha L.

2004-01-01T23:59:59.000Z

158

Structure and function of the human Poly(ADP-ribose) polymerase enzyme family  

E-Print Network (OSTI)

The poly(ADP-ribose) polymerase (PARP) family of enzymes in humans is comprised of 17 proteins. PARP-1, the first member of the family, synthesizes a large, complex post-translational modification, poly(ADP-ribose). While ...

Rood, Jennifer E. (Jennifer Evelyn)

2013-01-01T23:59:59.000Z

159

An Invitation for Family Fun at PPPL's Open House on June 1 ...  

NLE Websites -- All DOE Office Websites (Extended Search)

Invitation for Family Fun at PPPL's Open House on June 1 By Jeanne Jackson DeVoe May 13, 2013 Tweet Widget Facebook Like Google Plus One An Invitation for Family Fun at PPPL's Open...

160

New families of human regulatory RNA structures identified by comparative analysis of vertebrate genomes  

E-Print Network (OSTI)

Regulatory RNA structures are often members of families with multiple paralogous instances across the genome. Family members share functional and structural properties, which allow them to be studied as a whole, facilitating ...

Kellis, Manolis

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Phylogenetic Distribution of Potential Cellulases in Bacteria  

E-Print Network (OSTI)

Project Goals: The main goal of this project is to connect diverse microbial groups with the extracellular enzyme systems that catalyze the decay of organic material. We will also determine whether different groups of microbes and their enzymes respond to environmental changes, and whether they can recover from such changes. Finally, we will develop mathematical models to predict the responses of microbial communities and their associated functions under new environmental conditions. In most terrestrial ecosystems, the depolymerization of plant cell wall is the rate limiting step in the turnover of organic material. The composition of plant detritus is known to depend mainly on enzymes produced by microorganisms. This raises the question: which phylogenetic lineages of microorganisms can degrade plant cell wall material, including cellulose? To address this question, we compared the distribution of Glycoside Hydrolases (GH) potentially related to the cellulose degradation among 3744 bacteria. Some phylogenetic groups are especially rich in GHs whereas some are very poor. For example, in bacteria from the Bacteroidetes phylum ~40 GHs (from the families 1,

Genomic Science Awardee; Renaud Berlemont

2012-01-01T23:59:59.000Z

162

Identifying and characterizing the most significant ?-glucosidase of the novel species Aspergillus saccharolyticus  

Science Conference Proceedings (OSTI)

A newly discovered fungal species, Aspergillus saccharolyticus, was found to produce a culture broth rich in beta-glucosidase activity. In this present work, the main beta-glucosidase of A. saccharolyticus responsible for the efficient hydrolytic activity was identified, isolated, and characterized. Ion exchange chromatography was used to fractionate the culture broth, yielding fractions with high beta-glucosidase activity and only one visible band on an SDS-PAGE gel. Mass spectrometry analysis of this band gave peptide matches to beta-glucosidases from aspergilli. Through a PCR approach using degenerate primers and genome walking, a 2919 base pair sequence encoding the 860 amino acid BGL1 polypeptide was determined. BGL1 of A. saccharolyticus has 91% and 82% identity with BGL1 from Aspergillus aculeatus and BGL1 from Aspergillus niger, respectively, both belonging to Glycoside hydrolase family 3. Homology modeling studies suggested beta-glucosidase activity with preserved retaining mechanism and a wider catalytic pocket compared to other beta-glucosidases. The bgl1 gene was heterologously expressed in Trichoderma reesei QM6a, purified, and characterized by enzyme kinetics studies. The enzyme can hydrolyze cellobiose, pNPG, and cellodextrins. The enzyme showed good thermostability, was stable at 50C, and at 60C it had a half-life of approximately 6 hours.

Sorensen, Anette; Ahring, Birgitte K.; Lubeck, Mette; Ubhayasekera, Wimal; Bruno, Kenneth S.; Culley, David E.; Lubeck, Peter S.

2012-08-20T23:59:59.000Z

163

SOLAR MAGNETIC FIELD REVERSALS AND THE ROLE OF DYNAMO FAMILIES  

SciTech Connect

The variable magnetic field of the solar photosphere exhibits periodic reversals as a result of dynamo activity occurring within the solar interior. We decompose the surface field as observed by both the Wilcox Solar Observatory and the Michelson Doppler Imager into its harmonic constituents, and present the time evolution of the mode coefficients for the past three sunspot cycles. The interplay between the various modes is then interpreted from the perspective of general dynamo theory, where the coupling between the primary and secondary families of modes is found to correlate with large-scale polarity reversals for many examples of cyclic dynamos. Mean-field dynamos based on the solar parameter regime are then used to explore how such couplings may result in the various long-term trends in the surface magnetic field observed to occur in the solar case.

DeRosa, M. L. [Lockheed Martin Solar and Astrophysics Laboratory, 3251 Hanover St. B/252, Palo Alto, CA 94304 (United States); Brun, A. S. [Laboratoire AIM Paris-Saclay, CEA/Irfu Universite Paris-Diderot CNRS/INSU, F-91191 Gif-sur-Yvette (France); Hoeksema, J. T. [W. W. Hansen Experimental Physics Laboratory, Stanford University, Stanford, CA 94305 (United States)

2012-09-20T23:59:59.000Z

164

A novel family of small proteins that affect plant development  

Science Conference Proceedings (OSTI)

The DVL genes represent a new group of plant proteins that influence plant growth and development. Overexpression of DVL1, and other members of the DVL family, causes striking phenotypic changes. The DVL proteins share sequence homology in their C-terminal half. Point mutations in the C-terminal domain show it is necessary and deletion studies demonstrate the C-terminal domain is sufficient to confer the overexpression phenotypes. The phenotypes observed, and the conservation of the protein sequence in the plant kingdom, does suggest the DVL proteins have a role in modulating plant growth and development. Our working hypothesis is the DVL proteins function as regulators of cellular signaling pathways that control growth and development.

John Charles Walker

2011-04-29T23:59:59.000Z

165

Soft plastic bread packaging: lead content and reuse by families  

Science Conference Proceedings (OSTI)

The presence of lead in labels painted on soft plastic bread packaging was evaluated. Lead was detected on the outside of 17 of 18 soft plastic bread bags that were analyzed, with an average of 26 +/- 6 mg per bag with lead. Of 106 families questioned, 16 percent of respondents reported turning the bags inside out before reusing for food storage, thus putting food in contact with the lead paint. We estimate that a weak acid, such as vinegar, could readily leach 100 micrograms of lead from a painted plastic bag within 10 minutes. Further, lead and other metals painted on food packaging of any type becomes part of the municipal waste stream subject to incineration and to land-filling. The use of lead in packaging presents an unnecessary risk to public health.

Weisel, C.; Demak, M.; Marcus, S.; Goldstein, B.D. (Univ. of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway (USA))

1991-06-01T23:59:59.000Z

166

Monitoring conservative retrofits in single family buildings. Final technical report  

SciTech Connect

This study has provided detailed before-and-after information on the ambient and comfort conditions in nine single family buildings, and on the energy consumption of those buildings, for one or more energy conservation retrofits. The data were recorded in such a manner that as well as being able to determine the savings from the retrofits and the influence these retrofits have on the comfort conditions of the residence, the effects of the retrofits on time-of-day usage are also determinable. The following are included in appendices: a table of participant`s names, site addresses and retrofit; significant dates and appropriate comments; a day of data and an annotated data set; pre-retrofit and post-retrofit audit data sheets; and usage history.

Richardson, C.S.

1992-12-06T23:59:59.000Z

167

Structural conditionality of the piezoelectric properties of langasite family crystals  

Science Conference Proceedings (OSTI)

The atomic displacements upon isomorphic substitutions in crystals of the langasite family have been analyzed. The thermal parameters are determined and the probability density function of atoms is analyzed. Local potential energy minima are found which can be occupied by atoms under external effects. The contributions of cations in all four independent crystallographic positions and anions in all three such positions to the piezoelectric properties are established. One specific structural feature is the constant (at isomorphic substitutions) or possible (under external effects) but always opposite displacements of two cations along symmetry axis 2. Large cations in eight-vertex polyhedra make the main contribution to the piezoelectric properties. The cations in the tetrahedra on symmetry axis 2 weaken these properties. The cations in the octahedra in the origin of coordinates and in the tetrahedra on symmetry axes 3 only slightly affect the piezoelectricity.

Dudka, A. P., E-mail: dudka@ns.crys.ras.ru; Simonov, V. I. [Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)

2011-11-15T23:59:59.000Z

168

PG&E (Gas) - Multi-Family Residential Energy Efficiency Rebates |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

PG&E (Gas) - Multi-Family Residential Energy Efficiency Rebates PG&E (Gas) - Multi-Family Residential Energy Efficiency Rebates PG&E (Gas) - Multi-Family Residential Energy Efficiency Rebates < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Appliances & Electronics Water Heating Program Info Funding Source System Benefits Charge Expiration Date 12/31/2013 State California Program Type Utility Rebate Program Rebate Amount Clothes Washers (In-Unit): $50 Clothes Washers (Common Area): $150 Central System Water/Space Heating: $1,500/Unit Storage Water Heater: $200/Unit Boilers: $500/Unit Furnace: $150 - $300/Unit Provider Residential Programs Through the Rebates for Multi-Family Properties Program, PG&E offers prescriptive rebates for owners and managers of multi-family properties of

169

Data Set Models and Exponential Families in Statistical Physics and Beyond  

E-Print Network (OSTI)

The exponential family of models is defined in a general setting, not relying on probability theory. Some results of information geometry are shown to remain valid. Exponential families both of classical and of quantum mechanical statistical physics fit into the new formalism. Other less obvious applications are predicted. For instance, quantum states can be modeled as points in a classical phase space and the resulting model belongs to the exponential family.

Jan Naudts; Ben Anthonis

2011-11-02T23:59:59.000Z

170

A two-point G1 Hermite interpolating family of spirals  

Science Conference Proceedings (OSTI)

A one-parameter family of spirals that can match planar, two-point G^1 Hermite data is presented. These spirals can be used as an alternative to the biarc, which is also a one-parameter family of curves that can match two-point G^1 Hermite data. Some ... Keywords: 68U07, Family of spirals, Two-point G1 Hermite interpolation

D. S. Meek; D. J. Walton

2009-01-01T23:59:59.000Z

171

Be SMART Multi-Family Efficiency Loan Program (Maryland) | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Multi-Family Efficiency Loan Program (Maryland) Multi-Family Efficiency Loan Program (Maryland) Be SMART Multi-Family Efficiency Loan Program (Maryland) < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Home Weatherization Commercial Weatherization Sealing Your Home Cooling Construction Design & Remodeling Other Ventilation Manufacturing Heat Pumps Appliances & Electronics Commercial Lighting Lighting Water Heating Windows, Doors, & Skylights Maximum Rebate Not specified Program Info Funding Source American Recovery and Reinvestment Act (ARRA); State Energy Program State Maryland Program Type State Loan Program Rebate Amount Varies Provider Maryland Department of Housing and Community Development Note: The eligible technologies listed above are only examples of some

172

Daughterhood to motherhood: The impacts of childhood exposure to family violence on subsequent mothering.  

E-Print Network (OSTI)

??Research indicates children's exposure to family violence results in long-term negative consequences, hindering social skill development and impacting on the ability to function as healthy, (more)

Mendis, Kathy

2006-01-01T23:59:59.000Z

173

Using Availability Indicators to Enhance Context-Aware Family Communication Applications .  

E-Print Network (OSTI)

??Family conversation between homes is difficult to initiate at mutually agreeable times as neither participant has exact knowledge of the other's activities or intentions. Whether (more)

Nagel, Kristine Susanne

2006-01-01T23:59:59.000Z

174

New Progress on Application of NEUI400kA Family High Energy ...  

Science Conference Proceedings (OSTI)

Presentation Title, New Progress on Application of NEUI400kA Family High Energy Efficiency Aluminum Reduction Pot (HEEP) Technology. Author(s)...

175

DEVELOPMENT OF A PRODUCTION COST ESTIMATION FRAMEWORK TO SUPPORT PRODUCT FAMILY DESIGN  

E-Print Network (OSTI)

The main task of a product family designer is to decide the right components/design variables to share among products to maintain economies of scale with minimum sacrifice in the performance of each product in the family. The decisions are usually based on several criteria, but production cost is of primary concern. Estimating the production cost of a family of products involves both estimating the production cost of each product in the family and the costs incurred by common and variant components/design variables in the family. To estimate these costs consistently and accurately, we propose a production cost estimation framework to support product family design based on Activity-Based Costing (ABC) that consists of three stages: (1) allocation, (2) estimation, and (3) analysis. In the allocation stage, the production activities and resources needed to produce the entire products in a family are identified and classified with an activity table, a resource table, and a production flow. To help allocate product data for production, a product family structure is represented by a hierarchical classification of products that form the product family. In the estimation stage, production costs are estimated with cost estimation methods selected based on the type of information available. In the analysis stage,

Jaeil Park; Timothy W. Simpson

2004-01-01T23:59:59.000Z

176

Internet-based Family Therapy from the Perspective of the Therapist: A Qualitative Inquiry.  

E-Print Network (OSTI)

??The purpose of this qualitative, phenomenological study was to learn more about the process of Internet-based Family Therapy and to discover the advantages and disadvantages (more)

Hall, Tracy D.

2013-01-01T23:59:59.000Z

177

New family of tiny crystals glow bright in LED lights | ornl...  

NLE Websites -- All DOE Office Websites (Extended Search)

| ORNL | News | Features SHARE News Feature New family of tiny crystals glow bright in LED lights ORNL scientist probes atomic structure to improve materials' luminescence...

178

Moving Toward Zero Energy Homes: The Ultimate Family Home, Las Vegas, Nevada (Brochure)  

DOE Green Energy (OSTI)

Brochure describes the energy efficient and solar energy features of the Ultimate Family Home Zero Energy Home, participant in the Zero Energy Homes initiative.

Not Available

2003-12-01T23:59:59.000Z

179

Family literacypredictors of program participation and goal attainment in Kentucky.  

E-Print Network (OSTI)

??Ph. D. The purpose of this study was to investigate how various components of family literacy programs such as operational characteristics (enrollment procedure, hours of (more)

Harrison, Zelma Renae Stewart

2004-01-01T23:59:59.000Z

180

Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation  

E-Print Network (OSTI)

% oxygen and 5% carbon dioxide creating pH 7.4. Preparations were placed in SIF inside a thermo- static shaking bath at 32C for a 30 min washout- phase before the experiment. All experiments were performed with matched pairs of a treated and untreated side... these reports are difficult to reconcile, it is possible that differences in the mechanisms of immune cell activation between the two models may be a determinant factor since LPS and zymosan selectively activate toll like receptor 4 (TLR-4) and TLR-2...

Brenneis, Christian; Sisignano, Marco; Coste, Ovidiu; Altenrath, Kai; Fischer, Michael J; Angioni, Carlo; Fleming, Ingrid; Brandes, Ralf P; Reeh, Peter W; Woolf, Clifford J; Geisslinger, Gerd; Scholich, Klaus

2011-10-04T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

The Lorentz extension as consequence of the family symmetry  

SciTech Connect

In this paper, we postulate an algebraic model to explain how the symmetry of three lepton species plays its role in the Lorentz extension. Inspired by the two-to-one mapping between the SL(2,C) group and the Lorentz group, we design a mapping between the SL(3,C) group, which displays the family symmetry, and a generalized Lorentz group. Following the conventional method, we apply the mapping results to the Dirac equation to discuss its transformation invariance, and it turns out that only when the vertex matrix {gamma}{sub {mu}} is extended to the combination c{sub 1}{gamma}{sub {mu}}+c{sub 2}{gamma}{sub 5}{gamma}{sub {mu}} can the Dirac-equation form be reserved. At the same time, we find that the Lorentz group has to be extended with an additional generator {gamma}{sub 5}. The generalized vertex matrix is helpful in understanding the axial-like form of weak interaction and the neutrino oscillations.

Wang Haijun [Center for Theoretical Physics and School of Physics, Jilin University, Changchun 130023 (China)

2008-05-15T23:59:59.000Z

182

A Simple Family of Models for Eccentric Keplerian Fluid Disks  

E-Print Network (OSTI)

In order to be in a long-lived configuration, the density in a fluid disk should be constant along streamlines to prevent compressional (PdV) work from being done cyclically around every orbit. In a pure Kepler potential, flow along aligned, elliptical streamlines of constant eccentricity will satisfy this condition. For most density profiles, differential precession driven by the pressure gradient will destroy the alignment; however, in the razor-thin approximation there is a family of simple equilibria in which the precession frequency is the same at all radii. These disks may therefore be long-lived at significant eccentricities. The density can be made axisymmetric as r goes to 0, while maintaining the precession rate, by relaxing the requirement of constancy along streamlines in an arbitrarily small transition region near the center. In the limit of small eccentricity, the models can be seen as acoustically perturbed axisymmetric disks, and the precession rate is shown to agree with linear theory. The perturbation is a traveling wave similar to an ocean wave, with the fluid rising and falling epicyclically in the gravitational field of the central mass. The expected emission line profiles from the eccentric disks are shown to be strongly asymmetric in general, and, in extreme cases, prone to misinterpretation as single narrow lines with significant velocity offsets.

T. S. Statler

2001-08-06T23:59:59.000Z

183

F.C.D.A. FAMILY SHELTER EVALUATION  

SciTech Connect

In order to determine the effects of atomic explosions on small civil defense shelters for family use, 29 simple structures were built along an arc 1200 ft from the target point and exposed to Buster Bursts B, C, and D. The structures were of four basic types; covered-trench, metal-arch, wood-arch, and basement lean-to. Because of poor cohesive properties of the soil, much of the earth cover on the shelters was removed by the first shot. Since test procedures prevented restoration of structures and replacement of cover after each blast, test results were materially affected. Partly above-grade cover-trench shelters provided less protection against blast than belowgrade cover-trench shelters and were much less desirable as protection against gamma radiation. Metal-arch shelters set in concrete appeared to have good potentialities with minor design modifications. Wood-arch shelters as designed, proved to be unsuitable substitutes for metalarch shelters. No worthwhile information was obtained on basement lean-to shelters. Unusual conditions disclosed design deficiencies in entrance construction, front, and end sections, and effective earth cover. Small shelters are potentially capable of meeting requirements of civil defense. (auth)

Flynn, A.P.

1952-03-01T23:59:59.000Z

184

Pyrin gene and mutants thereof, which cause familial Mediterranean fever  

DOE Patents (OSTI)

The invention provides the nucleic acid sequence encoding the protein associated with familial Mediterranean fever (FMF). The cDNA sequence is designated as MEFV. The invention is also directed towards fragments of the DNA sequence, as well as the corresponding sequence for the RNA transcript and fragments thereof. Another aspect of the invention provides the amino acid sequence for a protein (pyrin) associated with FMF. The invention is directed towards both the full length amino acid sequence, fusion proteins containing the amino acid sequence and fragments thereof. The invention is also directed towards mutants of the nucleic acid and amino acid sequences associated with FMF. In particular, the invention discloses three missense mutations, clustered in within about 40 to 50 amino acids, in the highly conserved rfp (B30.2) domain at the C-terminal of the protein. These mutants include M6801, M694V, K695R, and V726A. Additionally, the invention includes methods for diagnosing a patient at risk for having FMF and kits therefor.

Kastner, Daniel L. (Bethesda, MD); Aksentijevichh, Ivona (Bethesda, MD); Centola, Michael (Tacoma Park, MD); Deng, Zuoming (Gaithersburg, MD); Sood, Ramen (Rockville, MD); Collins, Francis S. (Rockville, MD); Blake, Trevor (Laytonsville, MD); Liu, P. Paul (Ellicott City, MD); Fischel-Ghodsian, Nathan (Los Angeles, CA); Gumucio, Deborah L. (Ann Arbor, MI); Richards, Robert I. (North Adelaide, AU); Ricke, Darrell O. (San Diego, CA); Doggett, Norman A. (Santa Cruz, NM); Pras, Mordechai (Tel-Hashomer, IL)

2003-09-30T23:59:59.000Z

185

Making love in the network closet: the benefits and work of family videochat  

Science Conference Proceedings (OSTI)

In this paper, we explore the benefits of videochat for families and the corresponding work that home users engage in to make a video call run smoothly. We explore the varieties of social work required, including coordination work, presentation work, ... Keywords: family, home, home networking, identity, values, video, videochat, videoconferencing

Morgan G. Ames; Janet Go; Joseph 'Jofish' Kaye; Mirjana Spasojevic

2010-02-01T23:59:59.000Z

186

New taxa of oribatid mites of the family Liacaridae (Acariformes: Oribatida) from the Caucasus  

E-Print Network (OSTI)

the Caucasus (Georgia) are described: a new subgenus and species Adoristes (Gordeeviella) krivolutskyisubgNew taxa of oribatid mites of the family Liacaridae (Acariformes: Oribatida) from the Caucasus. 2009: New taxa of oribatid mites of the family Liacaridae (Acariformes: Oribatida) from the Caucasus

Arillo, Antonio Gabriel

187

Comparative analysis of the Epstein-Barr virus encoded nuclear proteins of EBNA-3 family  

Science Conference Proceedings (OSTI)

It is known that the EBNA-3 family proteins (EBNA-3, -4 and -6, alternative nomenclature EBNA-3A, B and C correspondingly) show a limited sequence similarity. We have analyzed EBNA-3 proteins both at the primary sequence and secondary structure levels. ... Keywords: Charge clusters, EBNA3 family proteins, Praline rich domain, Secondary structure analysis, Stonin homology domain

Surya Pavan Yenamandra; Ramakrishna Sompallae; George Klein; Elena Kashuba

2009-11-01T23:59:59.000Z

188

The Bayesian pursuit algorithm: a new family of estimator learning automata  

Science Conference Proceedings (OSTI)

The fastest Learning Automata (LA) algorithms currently available come from the family of estimator algorithms. The Pursuit algorithm (PST), a pioneering scheme in the estimator family, obtains its superior learning speed by using Maximum Likelihood ... Keywords: Bayesian learning automata, Bayesian pursuit algorithm, beta distribution, estimator algorithms, learning automata, pursuit algorithm

Xuan Zhang; Ole-Christoffer Granmo; B. John Oommen

2011-06-01T23:59:59.000Z

189

A matrix-based methodology for determining a part family's learning rate  

Science Conference Proceedings (OSTI)

Learning is a decrease in the time to perform an operation due to repetition and is an important consideration when forecasting process times or product costs. This paper presents a new method for calculating the learning rate for a family of parts using ... Keywords: Cost estimation, Family learning rate, Learning

William A. Young, II; Dale T. Masel; Robert P. Judd

2008-04-01T23:59:59.000Z

190

MicroSyn: A user friendly tool for detection of microsynteny in a gene family  

NLE Websites -- All DOE Office Websites (Extended Search)

O O F T W A R E Open Access MicroSyn: A user friendly tool for detection of microsynteny in a gene family Bin Cai 1,2 , Xiaohan Yang 3,4 , Gerald A Tuskan 3,4 and Zong-Ming Cheng 1,2,4* Abstract Background: The traditional phylogeny analysis within gene family is mainly based on DNA or amino acid sequence homologies. However, these phylogenetic tree analyses are not suitable for those "non-traditional" gene families like microRNA with very short sequences. For the normal protein-coding gene families, low bootstrap values are frequently encountered in some nodes, suggesting low confidence or likely inappropriateness of placement of those members in those nodes. Results: We introduce MicroSyn software as a means of detecting microsynteny in adjacent genomic regions surrounding genes in gene families. MicroSyn searches for conserved, flanking colinear homologous gene

191

"Feds Feed Families" Events to Be Held at Department of Energy Facilities |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

"Feds Feed Families" Events to Be Held at Department of Energy "Feds Feed Families" Events to Be Held at Department of Energy Facilities "Feds Feed Families" Events to Be Held at Department of Energy Facilities August 4, 2011 - 9:23am Addthis Deputy Secretary Daniel Poneman and his band "Yellow Cake" at last year's Feds Feed Families event. | Energy Department photo Deputy Secretary Daniel Poneman and his band "Yellow Cake" at last year's Feds Feed Families event. | Energy Department photo What are the key facts? Events to be held at Forrestal Building on August 4 from 11:00 a.m. - 2:00 p.m., and at the Germantown Building on August 11 from 11:00 a.m. - 2:00 p.m. Last year, the DOE exceeded its donation goal by 300 percent with 120,014 lbs. of donations. Deputy Secretary Daniel Poneman is in a band called "Yellow Cake".

192

SoCalGas - Multi-Family Residential Rebate Program | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Multi-Family Residential Rebate Program Multi-Family Residential Rebate Program SoCalGas - Multi-Family Residential Rebate Program < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Home Weatherization Commercial Weatherization Appliances & Electronics Construction Water Heating Program Info State California Program Type Utility Rebate Program Rebate Amount Dishwashers: $30 Insulation: 25% Natural Gas Storage Water Heaters: $30 Tankless Water Heaters: $300 Central Furnaces: $200 Central System Water Heaters: $500 Central System Boilers: $1,500 Central Demand Hot Water Controllers: $700 or $1400 Provider Southern California Gas Company Southern California Gas Company provides incentives to encourage the owners and managers of multi-family residential buildings to increase their energy

193

Market oriented approach to energy conservation identifying disadvantaged families as target groups for energy assistance programs  

SciTech Connect

Energy expenditures, perceived family well-being, and energy conservation actions were analyzed by family composition and income level. Data were taken from a three state subsample (Arizona, Colorado, and Oregon; N = 2633) of a larger stratified random sample of households in the Western US. Data were collected by mail survey in spring 1981. Self-reported annual energy expenditures were correlated with scores on an Index of Well Being, measuring the extent of cut-backs in several areas of consumption. No significant correlation was found between energy expenditures and the Index of Well Being. The proportion of income spent on residential energy, the energy budget share, however, was significantly correlated with the Index of Well Being. Families were classified according to income and also according to a modernize family-life-cycle (FLC) model, using age of the head of household, marital status, and family size. Significant differences between family types and income groups existed for energy expenditures, the energy budget share, as well as the Index of Well Being. There was no interaction between the two grouping factors, family type and income category, in the case of energy expenditures and scores on the Index of Well Being. A relationship between energy expenditures and climate, measured by heating and cooling degree days, was not found. The inverse relationship between heating and cooling requirements, as well as systematic differences in per unit energy cost appeared to account for this fact.

Marganus, M.G.

1984-01-01T23:59:59.000Z

194

On the origin of families of fermions and their mass matrices  

E-Print Network (OSTI)

We are proposing a new way of describing families of quarks and leptons, using the approach unifying all the internal degrees of freedom, proposed by one of us. Spinors, living in d(=1+13)-dimensional space, carry in this approach only the spin and interact with only the gravity through vielbeins and two kinds of the spin connection fields - the gauge fields of the Poincare group and the second kind of the Clifford algebra objects. All the quarks and the leptons of one family appear in one Weyl representation of a chosen handedness of the Lorentz group, if analyzed with respect to the Standard model gauge groups: the right handed (with respect to SO(1,3)) weak chargeless quarks and leptons and the left handed weak charged quark and leptons. A part of the starting Lagrange density of a Weyl spinor in d=1+13 transforms right handed quarks and leptons into left handed quarks and leptons manifesting as the Yukawa couplings of the Standard model. The second kind of Clifford algebra objects generates families and contributes to diagonal and off diagonal Yukawa couplings. The approach predicts an even number of families, treating leptons and quarks equivalently. In this paper we investigate within this approach the appearance of the Yukawa couplings within one family of quarks and leptons as well as among the families (without assuming any Higgs fields). We present the mass matrices for four families and investigate whether our way of generating families might explain the origin of families of quarks and leptons as well as their observed properties - the masses and the mixing matrices. Numerical results are presented in the paper following this one.

Anamarija Borstnik Bracic; Norma Susana Mankoc Borstnik

2005-12-05T23:59:59.000Z

195

Three Extra Mirror or Sequential Families: Case for a Heavy Higgs Boson and Inert Doublet  

Science Conference Proceedings (OSTI)

We study the possibility of the existence of extra fermion families and an extra Higgs doublet. We find that requiring the extra Higgs doublet to be inert leaves space for three extra families, allowing for mirror fermion families and a dark matter candidate at the same time. The emerging scenario is very predictive: It consists of a standard model Higgs boson, with a mass above 400 GeV, heavy new quarks between 340 and 500 GeV, light extra neutral leptons, and an inert scalar with a mass below M{sub Z}.

Martinez, Homero [CEA, Saclay, DSM-IRFU-SPP (France); Melfo, Alejandra [ICTP, Trieste (Italy); Universidad de Los Andes, Merida (Venezuela, Bolivarian Republic of); Nesti, Fabrizio [Universita di Ferrara, Ferrara (Italy); Senjanovic, Goran [ICTP, Trieste (Italy)

2011-05-13T23:59:59.000Z

196

NBER WORKING PAPER SERIES THE TEMPORARY ASSISTANCE FOR NEEDY FAMILIES PROGRAM  

E-Print Network (OSTI)

JEL No. I3 The Temporary Assistance for Needy Families (TANF) program was created in 1996 from what was previously named the Aid to Families with Dependent Children (AFDC) program. The TANF program is intended to serve low-income families, primarily those with only a single parent present, as did the AFDC program. The TANF program is distinguished from AFDC by strong work requirements, time limits on receipt, options for the provision of noncash assistance, and by a block grant financing structure. This paper reviews the rules of the TANF program and the research that has been conducted on it and on the AFDC program.

Robert A. Moffitt; Robert A. Moffitt

2002-01-01T23:59:59.000Z

197

Feds Feed Families Wraps Up Successful Campaign to Stock Area Food Banks |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Feds Feed Families Wraps Up Successful Campaign to Stock Area Food Feds Feed Families Wraps Up Successful Campaign to Stock Area Food Banks Feds Feed Families Wraps Up Successful Campaign to Stock Area Food Banks August 1, 2012 - 12:00pm Addthis EM’s Nevada Site Office took first place in the site-submitted category of DOE’s CANstruction Sculpture Contest for its entry, shown here, inspired by London’s Tower Bridge during the 2012 Summer Olympics. EM's Nevada Site Office took first place in the site-submitted category of DOE's CANstruction Sculpture Contest for its entry, shown here, inspired by London's Tower Bridge during the 2012 Summer Olympics. EM Office of Strategic Planning and Analysis Director Barry Gaffney throws the ball that sends Senior Advisor for Environmental Management David Huizenga into the dunk tank during an event to collect nonperishable food items for the DOE Feeds Families campaign.

198

SDG&E (Electric) - Multi-Family Residential Efficiency Program | Department  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Multi-Family Residential Efficiency Program Multi-Family Residential Efficiency Program SDG&E (Electric) - Multi-Family Residential Efficiency Program < Back Eligibility Multi-Family Residential Savings Category Home Weatherization Commercial Weatherization Heating & Cooling Cooling Commercial Heating & Cooling Appliances & Electronics Heat Pumps Commercial Lighting Lighting Water Heating Program Info State California Program Type Utility Rebate Program Rebate Amount Clothes Washers: $75-$150 Room Air Conditioner: $50 Central Heat Pumps: $100 Insulation: $0.15/sq. ft. CFLs: $4-$10 Ceiling Fans with CFLs: $20 Interior Hardwired Fluorescent Fixtures: $32-$45 Exterior Hardwired Fluorescent Fixtures: $30 T12 De-lamping: $6/lamp Water Heaters: $30 Occupancy Sensors: $10 LED Exit Signs: $35 Photocells: $10/unit

199

DOE Provides $96.4 Million to Low-Income Families for Home Weatherization |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

96.4 Million to Low-Income Families for Home 96.4 Million to Low-Income Families for Home Weatherization DOE Provides $96.4 Million to Low-Income Families for Home Weatherization July 6, 2006 - 2:50pm Addthis Funding is Second Installment of $243 Million in Total Weatherization Grants for FY 2006 WASHINGTON, D.C. - U.S. Department of Energy (DOE) Secretary Samuel W. Bodman today announced $96.4 million in weatherization program grants to 19 states to make energy efficiency improvements in homes of low-income families. Weatherization can reduce an average home's energy costs by $358 annually. Total Fiscal Year 2006 funding is $243 million and will provide weatherization to approximately 96,560 homes. "Weatherizing your home is a valuable way to save energy and money," Secretary Bodman said. "The Department of Energy's weatherization program

200

Sam's Family Spa Pool & Spa Low Temperature Geothermal Facility | Open  

Open Energy Info (EERE)

Sam's Family Spa Pool & Spa Low Temperature Geothermal Facility Sam's Family Spa Pool & Spa Low Temperature Geothermal Facility Jump to: navigation, search Name Sam's Family Spa Pool & Spa Low Temperature Geothermal Facility Facility Sam's Family Spa Sector Geothermal energy Type Pool and Spa Location Desert Hot Springs, California Coordinates 33.961124°, -116.5016784° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[]}

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Workforce Issues in the Greater Boston Health Care Industry: Implications for Work and Family  

E-Print Network (OSTI)

This working paper synthesizes critical problems identified by interviews with more than 40 leaders in the Boston area health care industry and places them in the context of work and family issues. At ...

Harrington, Mona

2004-12-10T23:59:59.000Z

202

PG&E - Multi-Family Residential Energy Efficiency Rebates | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

PG&E - Multi-Family Residential Energy Efficiency Rebates PG&E - Multi-Family Residential Energy Efficiency Rebates PG&E - Multi-Family Residential Energy Efficiency Rebates < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Home Weatherization Commercial Weatherization Cooling Appliances & Electronics Other Manufacturing Heat Pumps Commercial Lighting Lighting Insulation Design & Remodeling Water Heating Windows, Doors, & Skylights Program Info Expiration Date 03/01/2013 State California Program Type Utility Rebate Program Rebate Amount Attic Insulation: $0.15/sq. ft. Wall Insulation: $0.50/sq. ft. Clothes Washers: $50 - $150/Unit Refrigerator: $75/unit High Performance Dual Pane Windows: $0.75/sq. ft. Refrigerator, Freezer and Room AC Recycling: $25 - $35

203

Energy Department Provides $140.3 Million to Low-Income Families for Home  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

140.3 Million to Low-Income Families 140.3 Million to Low-Income Families for Home Weatherization Energy Department Provides $140.3 Million to Low-Income Families for Home Weatherization April 3, 2006 - 9:55am Addthis Funding is first installment of $243 million in total weatherization grants for FY 2006 WASHINGTON, D.C. - U.S. Department of Energy (DOE) Secretary Samuel W. Bodman today announced $140.3 million in weatherization program grants to 31 states and the Navajo Nation to make energy efficiency improvements in homes of low-income families; weatherization can reduce an average home's energy costs by $358 annually. Total Fiscal Year 2006 funding is $243 million and will provide weatherization to approximately 96,560 homes. "Weatherizing your home is a valuable way to save energy and money,"

204

Family-Owned Restaurant Serves Up Huge Energy Savings | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Family-Owned Restaurant Serves Up Huge Energy Savings Family-Owned Restaurant Serves Up Huge Energy Savings Family-Owned Restaurant Serves Up Huge Energy Savings May 8, 2013 - 2:27pm Addthis Energy efficiency upgrades helped the Athenian Corner reduce its operating costs and improved the restaurant's bottom line. | Photo courtesy of BetterBuildings Lowell Energy Upgrade program. Energy efficiency upgrades helped the Athenian Corner reduce its operating costs and improved the restaurant's bottom line. | Photo courtesy of BetterBuildings Lowell Energy Upgrade program. Rebecca Matulka Rebecca Matulka Digital Communications Specialist, Office of Public Affairs What are the key facts? The Athenian Corner, a family-owned restaurant in Lowell, Massachusetts, made energy efficiency upgrades that are saving it more than

205

Y-12 employees make holiday merry for local families | National Nuclear  

NLE Websites -- All DOE Office Websites (Extended Search)

employees make holiday merry for local families | National Nuclear employees make holiday merry for local families | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > Y-12 employees make holiday merry for local families Y-12 employees make holiday merry for local families Posted By Office of Public Affairs Nearly 50 Y-12 employees served as Santa's elves in the 2012 Angel Tree

206

How Do You Encourage Your Family to Use Less Water? | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Encourage Your Family to Use Less Water? Encourage Your Family to Use Less Water? How Do You Encourage Your Family to Use Less Water? October 29, 2009 - 9:00am Addthis On Monday, Allison discussed fifteen ways you can save on your water heating costs. While there are many ways to keep your HOT water use to a minimum, sometimes the best thing to do is just shut off the water as much as possible! But if you have multiple people living in your home, it can be difficult to convince everyone to do their part to use less water. Maybe one likes to linger in a hot shower while another leaves the water running while brushing his teeth-all of these little habits add up! How do you encourage your family to use less water? Each Thursday, you have the chance to share your thoughts on a question about energy efficiency or renewable energy for consumers. Please comment

207

Y-12 employees make holiday merry for local families | National Nuclear  

National Nuclear Security Administration (NNSA)

employees make holiday merry for local families | National Nuclear employees make holiday merry for local families | National Nuclear Security Administration Our Mission Managing the Stockpile Preventing Proliferation Powering the Nuclear Navy Emergency Response Recapitalizing Our Infrastructure Continuing Management Reform Countering Nuclear Terrorism About Us Our Programs Our History Who We Are Our Leadership Our Locations Budget Our Operations Media Room Congressional Testimony Fact Sheets Newsletters Press Releases Speeches Events Social Media Video Gallery Photo Gallery NNSA Archive Federal Employment Apply for Our Jobs Our Jobs Working at NNSA Blog Home > NNSA Blog > Y-12 employees make holiday merry for local families Y-12 employees make holiday merry for local families Posted By Office of Public Affairs Nearly 50 Y-12 employees served as Santa's elves in the 2012 Angel Tree

208

Cost-effectiveness analysis of genetic testing for familial long QT syndrome in symptomatic index cases  

E-Print Network (OSTI)

Cost-Effectiveness Analysis of Genetic Testing for FamilialJ, Russell L, Weinstein M. Cost-effectiveness in health andSakowski J: An introduction to cost- effectiveness and cost-

Phillips, Kathryn A; Ackerman, M J; Sakowski, J; Berul, C I

2005-01-01T23:59:59.000Z

209

Spontaneous generation of prion infectivity in fatal familial insomnia knock-in mice  

E-Print Network (OSTI)

A crucial tenet of the prion hypothesis is that misfolding of the prion protein (PrP) induced by mutations associated with familial prion disease is, in an otherwise normal mammalian brain, sufficient to generate the ...

Faas, Henryk

210

Molecular Basis for Specific Regulation of Neuronal Kinesin-3 Motors by Doublecortin Family Proteins  

E-Print Network (OSTI)

Doublecortin (Dcx) defines a growing family of microtubule (MT)-associated proteins (MAPs) involved in neuronal migration and process outgrowth. We show that Dcx is essential for the function of Kif1a, a kinesin-3 motor ...

Liu, JudyS.

211

Front/back/side : design of outdoor spaces for multi-family residences  

E-Print Network (OSTI)

This thesis begins with the critique of the current situation of detached, privately owned, multi-family residences and seeks alternative solutions at both the broad scale of the block, and at the scale of the spaces ...

Darden, Gwynne

1996-01-01T23:59:59.000Z

212

University of Pittsburgh Early Head Start (Family Foundations) is a 15-year program that  

E-Print Network (OSTI)

. The program serves 295 children or expectant families through a home-based mod- el and 15 infants and toddlers serves 295 children in a home-based environment so that parents spend time with their young child while

Sibille, Etienne

213

A family of complete caps in PG(n,2) - CECM  

E-Print Network (OSTI)

P. Lisonek, M. Khatirinejad, A family of complete caps in PG(n,2). Designs, Codes and Cryptography 35 (2005), 259-270. We give a combinatorial construction of...

214

A FAMILY TREE OF TROPICAL METEOROLOGY'S ACADEMIC COMMUNITY AND ITS PROPOSED EXPANSION  

Science Conference Proceedings (OSTI)

As part of the 30th American Meteorological Society Conference on Hurricanes and Tropical Meteorology in Ponte Vedra Beach, Florida in April 2012, an academic lineage (family tree) of that community was presented to document the history of contributors ...

ROBERT E. HART; JOSHUA H. COSSUTH

215

A comparative study of condominium and single family house price appreciation in the Salt Lake Valley  

E-Print Network (OSTI)

This study examines whether the form of ownership affects the appreciation rate of housing units. The specific test conducted is whether condominiums and single family homes in the Salt Lake Valley have appreciated at the ...

Billings, John D. (John David), 1971-

2003-01-01T23:59:59.000Z

216

Passion and Compassion: Psychology of Kin Relations within and Beyond the Family  

E-Print Network (OSTI)

Family is special. People avoid sexual contact with close relatives, but at the same time are highly beneficent toward them. Such discriminatory behavior is guided by a set of psychological mechanisms, heuristics that ...

Ackerman, Joshua

217

Higgs Mass Constraints on a Fourth Family: Upper and Lower Limits on CKM Mixing  

E-Print Network (OSTI)

June 25, 2010 Higgs Mass Constraints on a Fourth Family:and experimental limits on the Higgs boson mass restrict CKMGeV lower limit on the Higgs boson mass that is especially

Chanowitz, Michael S.

2010-01-01T23:59:59.000Z

218

The impact of multifamily development on single family home prices in the Greater Boston Area  

E-Print Network (OSTI)

The impact of large, multifamily developments on nearby single-family home prices was tested in five towns in the Greater Boston Area. Case studies that had recent multifamily developments built near transit nodes or town ...

Schuur, Arah (Arah Louise Adele)

2005-01-01T23:59:59.000Z

219

A sub-systems approach to small lot single-family housing  

E-Print Network (OSTI)

The trends and preferences explored in this work indicate that the "American Dream" of a single-family detached house is still the preferred housing model. In-order to achieve this goal most home buyers will have to accept ...

Khn, Heinrich, 1951-

1988-01-01T23:59:59.000Z

220

Working Families Tax Relief Act of 2004 (released in AEO2005)  

Reports and Publications (EIA)

The Working Families Tax Relief Act of 2004 was signed into law on October 13, 2004. Primarily, the Act reduces taxes for individuals and businesses. At least two provisions relate to energy: Depletion of marginal properties and qualified vehicles.

Information Center

2005-02-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

A Nuclear Family: I've Seen It | Y-12 National Security Complex  

NLE Websites -- All DOE Office Websites (Extended Search)

I've ... A Nuclear Family: I've Seen It The mp4 video format is not supported by this browser. Download video Captions: On Time: 26:42 min. Episode 1 shows how the lives of East...

222

Analysis of advanced conceptual designs for single-family-size absorption chillers  

DOE Green Energy (OSTI)

The objective of this research study is the development of radically new fluid systems, specifically tailored to the needs and requirements of solar-absorption cooling for single-family-size residences. Progress is reported.

Macriss, R.A.; Zawacki, T.S.; Kouo, M.T.; Sneed, D.M.

1978-01-01T23:59:59.000Z

223

The More the Merrier? The Effect of Family Size and Birth Order on Children's Education  

E-Print Network (OSTI)

estimate effects of birth order She does not control for agemagnitude of the birth order effects does not differ muchvariables does not affect our family size or birth order

Black, Sandra E; Devereux, Paul J.; Salvanes, Kjell

2005-01-01T23:59:59.000Z

224

ConEd (Gas) - Multi-family Energy Efficiency Incentives Program |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

ConEd (Gas) - Multi-family Energy Efficiency Incentives Program ConEd (Gas) - Multi-family Energy Efficiency Incentives Program ConEd (Gas) - Multi-family Energy Efficiency Incentives Program < Back Eligibility Multi-Family Residential Savings Category Heating & Cooling Commercial Heating & Cooling Heating Home Weatherization Commercial Weatherization Sealing Your Home Ventilation Construction Manufacturing Appliances & Electronics Water Heating Maximum Rebate Steam Boiler: $2500 Energy Management System: 70% of total cost Program Info Expiration Date 12/31/2015 State New York Program Type Utility Rebate Program Rebate Amount Hot Water Gas Boilers (85%-89% TE): $1000-$3500/boiler Hot Water Gas Condensing Boilers (90%+ TE): $2000-$15,000/boiler Gas Steam Boilers: $700/boiler (300 MBH) Heating System Clean and Tune: $225/boiler

225

An interactive particle swarm optimisation for selecting a product family and designing its supply chain  

Science Conference Proceedings (OSTI)

A platform-based product development with mixed market-modular strategy can maintain product differentiation and help trade-off the cost and price premium drawing capability. This paper formulates a multi-objective problem to select a ... Keywords: AGVs, SCM, automated guided vehicles, interactive PSO, market strategy, modular products, particle swarm optimisation, platform-based product development, process flexibility, product differentiation, product families, product family selection, supply chain design, supply chain management, wiring harness suppliers

S. R. Yadav; Yogesh Dashora; Ravi Shankar; Felix. T. S. Chan; M. K. Tiwari

2008-05-01T23:59:59.000Z

226

The effect of incarceration rates on mate availability and its effect on the formation and structure of the African American family: a theoretical and empirical analysis.  

E-Print Network (OSTI)

??A number of scholars have argued that declining mate availability for African American women has had important consequences for family formation and family structure in (more)

McGruder, Patricia Hollis

2012-01-01T23:59:59.000Z

227

The Sorcerer II Global Ocean Sampling Expedition: Expanding theUniverse of Protein Families  

SciTech Connect

Metagenomics projects based on shotgun sequencing of populations of micro-organisms yield insight into protein families. We used sequence similarity clustering to explore proteins with a comprehensive dataset consisting of sequences from available databases together with 6.12 million proteins predicted from an assembly of 7.7 million Global Ocean Sampling (GOS) sequences. The GOS dataset covers nearly all known prokaryotic protein families. A total of 3,995 medium- and large-sized clusters consisting of only GOS sequences are identified, out of which 1,700 have no detectable homology to known families. The GOS-only clusters contain a higher than expected proportion of sequences of viral origin, thus reflecting a poor sampling of viral diversity until now. Protein domain distributions in the GOS dataset and current protein databases show distinct biases. Several protein domains that were previously categorized as kingdom specific are shown to have GOS examples in other kingdoms. About 6,000 sequences (ORFans) from the literature that heretofore lacked similarity to known proteins have matches in the GOS data. The GOS dataset is also used to improve remote homology detection. Overall, besides nearly doubling the number of current proteins, the predicted GOS proteins also add a great deal of diversity to known protein families and shed light on their evolution. These observations are illustrated using several protein families, including phosphatases, proteases, ultraviolet-irradiation DNA damage repair enzymes, glutamine synthetase, and RuBisCO. The diversity added by GOS data has implications for choosing targets for experimental structure characterization as part of structural genomics efforts. Our analysis indicates that new families are being discovered at a rate that is linear or almost linear with the addition of new sequences, implying that we are still far from discovering all protein families in nature.

Yooseph, Shibu; Sutton, Granger; Rusch, Douglas B.; Halpern,Aaron L.; Williamson, Shannon J.; Remington, Karin; Eisen, Jonathan A.; Heidelberg, Karla B.; Manning, Gerard; Li, Weizhong; Jaroszewski, Lukasz; Cieplak, Piotr; Miller, Christopher S.; Li, Huiying; Mashiyama, Susan T.; Joachimiak, Marcin P.; van Belle, Christopher; Chandonia, John-Marc; Soergel, David A.; Zhai, Yufeng; Natarajan, Kannan; Lee, Shaun; Raphael,Benjamin J.; Bafna, Vineet; Friedman, Robert; Brenner, Steven E.; Godzik,Adam; Eisenberg, David; Dixon, Jack E.; Taylor, Susan S.; Strausberg,Robert L.; Frazier, Marvin; Venter, J.Craig

2006-03-23T23:59:59.000Z

228

The family of terpene synthases in plants: a midsize family of genes for specialized metabolism that is highly diversified throughout the kingdom  

NLE Websites -- All DOE Office Websites (Extended Search)

PLANT PLANT GENOME: AN EVOLUTIONARY VIEW ON STRUCTURE AND FUNCTION The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom Feng Chen 1,* , Dorothea Tholl 2 , Jo ¨ rg Bohlmann 3 and Eran Pichersky 4 1 Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA, 2 Department of Biological Sciences, 408 Latham Hall, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA, 3 Michael Smith Laboratories, 2185 East Mall, University of British Columbia, Vancouver, BC V6T 1Z4, Canada, and 4 Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA Received 14 October 2010; revised 19 January 2011; accepted 31 January 2011. * For correspondence (fax +1 865 974 1947; e-mail fengc@utk.edu). SUMMARY Some plant

229

Department of Energy Provides Nearly $112 Million to Low-Income Families  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

112 Million to Low-Income 112 Million to Low-Income Families for Home Weatherization Department of Energy Provides Nearly $112 Million to Low-Income Families for Home Weatherization March 29, 2007 - 12:17pm Addthis Funding is First Installment of $204.5 Million in Total Weatherization Grants for FY 2007 WASHINGTON, DC - U.S. Department of Energy (DOE) today announced $111.6 million in weatherization grants to 30 states and the Navajo Nation to make energy efficiency improvements in homes of low-income families. Weatherization can reduce an average home's energy costs by $358 annually. Total Fiscal Year 2007 funding is $204.5 million and will provide weatherization to approximately 70,000 homes. "Weatherization is a valuable way to help save money and energy," DOE Assistant Secretary for Energy Efficiency and Renewable Energy Alexander

230

Does Tracking Affect the Importance of Family Background on Students Test Scores?  

E-Print Network (OSTI)

This study investigates whether tracking students according to ability affects the importance of family background on students educational test scores. Using data from the PISA 2003, PISA 2000 and the PIRLS 2001 studies this paper uses the cross-country variation in tracking policies to identify the effect of tracking. The results indicate that family background is more important in countries, which track students early in a simple cross section. Using a difference-in-differences methodology to control for unobserved country level variables I find, however, that the importance of family background does not increase after actual tracking has taken place. This result is very different to the findings of two concurrent papers using a similar approach. Both of these papers nd that tracking affects educational equity. Using a number of robustness checks, however, I find that the results presented in this research are robust to using different tracking measures, datasets and specications.

Fabian Waldinger

2005-01-01T23:59:59.000Z

231

Atmospheric performance of the special-purpose Solar Energy Research Institute (SERI) thin-airfoil family  

DOE Green Energy (OSTI)

The Solar Energy Research Institute (SERI), in cooperation with SeaWest Energy Group, has completed extensive atmospheric testing of the special-purpose SERI thin-airfoil family during the 1990 wind season. The purpose of this test program was to experimentally verify the predicted performance characteristics of the thin-airfoil family on a geometrically optimized blade, and to compare it to original-equipment blades under atmospheric wind conditions. The tests were run on two identical Micon 65/13 horizontal-axis wind turbines installed side-by-side in a wind farm. The thin-airfoil family 7.96 m blades were installed on one turbine, and AeroStar 7.41 m blades were installed on the other. This paper presents final performance results of the side-by-side comparative field test for both clean and dirty blade conditions. 7 refs., 11 figs., 1 tab.

Tangler, J; Smith, B; Jager, D; Olsen, T

1990-09-01T23:59:59.000Z

232

10,000th Waste Shipment Milestone is All in the Family | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

10,000th Waste Shipment Milestone is All in the Family 10,000th Waste Shipment Milestone is All in the Family 10,000th Waste Shipment Milestone is All in the Family August 29, 2011 - 12:00pm Addthis A Waste Isolation Pilot Plant facility shift manager inspects the shipping manifest of the 10,000th safe shipment to roll through the gates at the Department of Energy’s Waste Isolation Pilot Plant on Sept. 24, 2011. A Waste Isolation Pilot Plant facility shift manager inspects the shipping manifest of the 10,000th safe shipment to roll through the gates at the Department of Energy's Waste Isolation Pilot Plant on Sept. 24, 2011. Idaho's Advanced Mixed Waste Treatment Project employees prepare to send the 10,000th shipment to the Waste Isolation Pilot Plant. Idaho's Advanced Mixed Waste Treatment Project employees prepare to send

233

Get the Family in on the Energy-Saving Act | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Get the Family in on the Energy-Saving Act Get the Family in on the Energy-Saving Act Get the Family in on the Energy-Saving Act April 21, 2010 - 7:30am Addthis Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs It's no wonder that many of Earth Day's most ardent supporters are children. Environmental education is growing into a fixture in classrooms across the United States. Students are often eager to put their lessons in sustainability into practice at home. In turn, children are often the ones to push their parents towards energy efficiency. Influential as they may be, meeting their requests for solar panels and hybrid cars is quite a tall order. The Department of Energy (DOE) has several websites designed specifically for environmentally conscious kids. Visit Lose Your Excuse to download an easy energy action plan. Go over the

234

Alabama Family Staying Nice and Cozy This Fall | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Alabama Family Staying Nice and Cozy This Fall Alabama Family Staying Nice and Cozy This Fall Alabama Family Staying Nice and Cozy This Fall December 2, 2009 - 5:49pm Addthis Joshua DeLung What are the key facts? The Community Action Agency of Talladega, Clay, Calhoun, Randolph and Cleburne Counties received about $1.8 million in Recovery Act money to weatherize homes and is assessing 60 to 70 homes each month. Mary's energy bill is about $300 cheaper now, and she no longer has to take extreme measures to keep her and her three children and grandchild warm. In the winter months, Mary Taylor felt a chilly draft billowing through her Talladega, Ala., home. The energy bills were mounting up higher and higher. In the summer, the sweltering southern heat was too much for her air conditioner to handle, as the cool air leaked out of house as quickly as

235

Critical Question #7: What are the Best Practices for Single-Family  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

7: What are the Best Practices for Single-Family 7: What are the Best Practices for Single-Family Ventilation in All Climate Regions? Critical Question #7: What are the Best Practices for Single-Family Ventilation in All Climate Regions? Why ventilate? What are the ultimate goals of ventilation requirements in codes and standards? What are the characteristics of an effective ventilation system in new vs. existing construction? What are the risks and solutions associated with ventilation in hot-humid climates? cq7_kitchen_ventilation_singer.pdf cq7_ventilation_lab_houses_rudd.pdf cq7_ventilation_hothumid_parker.pdf More Documents & Publications Track B - Critical Guidance for Peak Performance Homes Track C - Market-Driven Research Solutions Critical Question #8: When are Heat Pump Water Heaters the Best Solution?

236

Department of Energy Provides Nearly $88 Million to Low-Income Families for  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

88 Million to Low-Income 88 Million to Low-Income Families for Home Weatherization Department of Energy Provides Nearly $88 Million to Low-Income Families for Home Weatherization June 29, 2007 - 2:36pm Addthis Funding is Second Installment of $200 Million in Total Weatherization Grants for FY 2007 WASHINGTON, DC - U.S. Department of Energy (DOE) today announced $88 million in weatherization grants to 20 states to make energy efficiency improvements in homes of low-income families. Weatherization can reduce an average home's energy costs by $358 annually, and this year, DOE expects funding to weatherize approximately 70,000 homes nationwide. For every dollar spent, weatherization returns $1.53 in energy savings over the life of the measures. DOE's weatherization program performs energy

237

Homogenization of a thin plate reinforced with periodic families of rigid rods  

SciTech Connect

The asymptotics of the solution to the elastic bending problem for a thin plate reinforced with several periodic families of closely spaced but disjoint rods are constructed and justified, the result of homogenization being substantially different from the case when the rods are welded together into a single periodic mesh. The material in the rods is assumed to be appreciably more rigid than that in the plate. An averaged fourth-order differential operator is obtained from summing the nonelliptic operators generated by each of the families of the rods. This operator is shown to be elliptic if and only if the rods from at least two families are nonparallel. As a simplified example, the paper examines a similar stationary heat conduction problem. Bibliography: 24 titles.

Nazarov, Sergei A; Sweers, Guido H; Slutskij, Andrey S

2011-08-31T23:59:59.000Z

238

On a family of test statistics for discretely observed diffusion processes  

E-Print Network (OSTI)

We consider parametric hypotheses testing for multidimensional ergodic diffusion processes observed at discrete time. We propose a family of test statistics, related to the so called $\\phi$-divergence measures. By taking into account the quasi-likelihood approach developed for studying the stochastic differential equations, it is proved that the tests in this family are all asymptotically distribution free. In other words, our test statistics weakly converge to the chi squared distribution. Furthermore, our test statistic is compared with the quasi likelihood ratio test. In the case of contiguous alternatives, it is also possible to study in detail the power function of the tests. Although all the tests in this family are asymptotically equivalent, we show by Monte Carlo analysis that, in the small sample case, the performance of the test strictly depends on the choice of the function $\\phi$. Furthermore, in this framework, the simulations show that there are not uniformly most powerful tests.

De Gregorio, Alessandro

2011-01-01T23:59:59.000Z

239

Weatherized Homes Saving Money for Families Across the U.S. | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Weatherized Homes Saving Money for Families Across the U.S. Weatherized Homes Saving Money for Families Across the U.S. Weatherized Homes Saving Money for Families Across the U.S. January 6, 2012 - 4:19pm Addthis Olaf Sander and Zump Urycki evaluate the heating system of a home in Loveland, Colorado, as part of the Weatherization Assistance Program. | Credit: Dennis Schroeder, NREL. Olaf Sander and Zump Urycki evaluate the heating system of a home in Loveland, Colorado, as part of the Weatherization Assistance Program. | Credit: Dennis Schroeder, NREL. April Saylor April Saylor Former Digital Outreach Strategist, Office of Public Affairs What does this mean for me? 750 thousand homes weatherized by the Weatherization Assistance Program in three years. The program is saving the average household weatherized $400 a year

240

Get the Family in on the Energy-Saving Act | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Get the Family in on the Energy-Saving Act Get the Family in on the Energy-Saving Act Get the Family in on the Energy-Saving Act April 21, 2010 - 7:30am Addthis Erin R. Pierce Erin R. Pierce Digital Communications Specialist, Office of Public Affairs It's no wonder that many of Earth Day's most ardent supporters are children. Environmental education is growing into a fixture in classrooms across the United States. Students are often eager to put their lessons in sustainability into practice at home. In turn, children are often the ones to push their parents towards energy efficiency. Influential as they may be, meeting their requests for solar panels and hybrid cars is quite a tall order. The Department of Energy (DOE) has several websites designed specifically for environmentally conscious kids. Visit Lose Your Excuse to download an easy energy action plan. Go over the

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241

Department of Energy Provides Nearly $88 Million to Low-Income Families for  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Provides Nearly $88 Million to Low-Income Provides Nearly $88 Million to Low-Income Families for Home Weatherization Department of Energy Provides Nearly $88 Million to Low-Income Families for Home Weatherization June 29, 2007 - 2:36pm Addthis Funding is Second Installment of $200 Million in Total Weatherization Grants for FY 2007 WASHINGTON, DC - U.S. Department of Energy (DOE) today announced $88 million in weatherization grants to 20 states to make energy efficiency improvements in homes of low-income families. Weatherization can reduce an average home's energy costs by $358 annually, and this year, DOE expects funding to weatherize approximately 70,000 homes nationwide. For every dollar spent, weatherization returns $1.53 in energy savings over the life of the measures. DOE's weatherization program performs energy

242

Retiree Heads Back to Work, Helps Families Stay Afloat | Department of  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Retiree Heads Back to Work, Helps Families Stay Afloat Retiree Heads Back to Work, Helps Families Stay Afloat Retiree Heads Back to Work, Helps Families Stay Afloat November 10, 2009 - 7:17pm Addthis Mark Morris inspects a furnace. | File photo Mark Morris inspects a furnace. | File photo Joshua DeLung Getting laid off wasn't the only thing putting Americans in a pickle when the recession hit. Mark Morris of South Bend, Ind., is among many in the country who were already retired, but whose 401(k) account took a big hit when the stock market crashed. Mark had retired as a utility-company supervisor in mid-2007, but he lost 40 percent of his retirement money and knew he had to go back to work. So Mark made what he could by working part-time gigs until he heard about a job in September with a local community action agency where he would

243

Listing minimal edge-covers of intersecting families with applications to connectivity problems  

Science Conference Proceedings (OSTI)

Let G=(V,E) be a directed/undirected graph, let s,t@?V, and let F be an intersecting family on V (that is, X@?Y,X@?Y@?F for any intersecting X,Y@?F) so that s@?X and t@?X for every X@?F. An edge set I@?E is an edge-cover of F if for every X@?F there ... Keywords: Intersecting families, Listing, Minimal edge-covers, Steiner network

Zeev Nutov

2009-01-01T23:59:59.000Z

244

The Distin Family and its Influence on the Development of the Brass Band Movement in Nineteenth-Century Britain.  

E-Print Network (OSTI)

??This dissertation examines the influence of the Distin Family brass ensemble on the developing brass band movement in nineteenth-century Britain. As well as drawing critically (more)

FARR, RAYMOND,KENNETH

2012-01-01T23:59:59.000Z

245

Unpacking the opportunities for change within a family owned manufacturing sme : a design led innovation case study.  

E-Print Network (OSTI)

??This thesis explored how an Australian, family owned, manufacturing firm responded to a design led innovation approach as conducted by the action researcher. Specifically, it (more)

Pozzey, Erica C.

2013-01-01T23:59:59.000Z

246

Characteristics, Welfare Use and Material Hardship Among California AFDC Households with Disabled and Chronically Ill Family Members  

E-Print Network (OSTI)

completed telephone survey o f AFDC-recipient households tocare for disabled members. When AFDC and SSI are consideredfamilies in this sample of AFDC recipient families were very

Meyers, Marcia k.

1996-01-01T23:59:59.000Z

247

Complete genome of the cellyloytic thermophile Acidothermus cellulolyticus 11B provides insights into its ecophysiological and evloutionary adaptations  

SciTech Connect

We present here the complete 2.4 Mb genome of the cellulolytic actinobacterial thermophile, Acidothermus cellulolyticus 11B. New secreted glycoside hydrolases and carbohydrate esterases were identified in the genome, revealing a diverse biomass-degrading enzyme repertoire far greater than previously characterized, and significantly elevating the industrial value of this organism. A sizable fraction of these hydrolytic enzymes break down plant cell walls and the remaining either degrade components in fungal cell walls or metabolize storage carbohydrates such as glycogen and trehalose, implicating the relative importance of these different carbon sources. A novel feature of the A. cellulolyticus secreted cellulolytic and xylanolytic enzymes is that they are fused to multiple tandemly arranged carbohydrate binding modules (CBM), from families 2 and 3. Interestingly, CBM3 was found to be always N-terminal to CBM2, suggesting a functional constraint driving this organization. While the catalytic domains of these modular enzymes are either diverse or unrelated, the CBMs were found to be highly conserved in sequence and may suggest selective substrate-binding interactions. For the most part, thermophilic patterns in the genome and proteome of A. cellulolyticus were weak, which may be reflective of the recent evolutionary history of A. cellulolyticus since its divergence from its closest phylogenetic neighbor Frankia, a mesophilic plant endosymbiont and soil dweller. However, ribosomal proteins and non-coding RNAs (rRNA and tRNAs) in A. cellulolyticus showed thermophilic traits suggesting the importance of adaptation of cellular translational machinery to environmental temperature. Elevated occurrence of IVYWREL amino acids in A. cellulolyticus orthologs compared to mesophiles, and inverse preferences for G and A at the first and third codon positions also point to its ongoing thermoadaptation. Additional interesting features in the genome of this cellulolytic, hot-springs dwelling prokaryote include a low occurrence of pseudogenes or mobile genetic elements, an unexpected complement of flagellar genes, and presence of three laterally-acquired genomic islands of likely ecophysiological value.

Barabote, Ravi D.; Xie, Gary; Leu, David H.; Normand, Philippe; Necsulea, Anamaria; Daubin, Vincent; Medigue, Claudine; Adney, William S.; Xu,Xin Clare; Lapidus, Alla; Detter, Chris; Pujic, Petar; Bruce, David; Lavire, Celine; Challacombe, Jean F.; Brettin, Thomas S.; Berry, Alison M.

2009-01-01T23:59:59.000Z

248

Leveraging Mobility: Building Wealth, Security and Opportunity for Family Well-Being  

E-Print Network (OSTI)

Challenges in Leveraging Mobility In 1998 Michelle and Bob Johnson were part of a new African American middleLeveraging Mobility: Building Wealth, Security and Opportunity for Family Well-Being Hannah Thomas, Janet Boguslaw, Alexis Mann, and Thomas Shapiro November 2013 Leveraging Mobility Series #12;The Real

Snider, Barry B.

249

Employment Of Fuzzy Logic In Feature Diagrams To Model Variability In Software Families  

Science Conference Proceedings (OSTI)

The features of software family members may vary according to the needs of particular market segments or purposes. The assessment of what will be common and what will be variable feature may result from particular needs and market strategies. Further, ... Keywords: FODA feature diagrams, feature diagram tree, fuzzy representation, knowledge base, power plant description

Silva Robak; Andrzej Pieczy?ski

2003-08-01T23:59:59.000Z

250

Distributional Energy-Momentum Tensor of the Kerr-Newman Space-Time Family  

E-Print Network (OSTI)

Using the Kerr-Schild decomposition of the metric tensor that employs the algebraically special nature of the Kerr-Newman space-time family, we calculate the energy-momentum tensor. The latter turns out to be a well-defined tensor-distribution with disk-like support.

Herbert Balasin; Herbert Nachbagauer

1993-12-17T23:59:59.000Z

251

Collection of family health histories: The link between genealogy and public health  

Science Conference Proceedings (OSTI)

Although a number of investigations have been conducted on the information behavior of family historians, we know little about the degree to which they systematically collect information on the causes of death and major illnesses of ancestors. Such information, ... Keywords: biomedical information, data collection, genealogy, information resources, medical records

Donald O. Case

2008-12-01T23:59:59.000Z

252

Invariants of Families of Coplanar Conics and Their Applicationsto Object Recognition  

Science Conference Proceedings (OSTI)

This paper presents mutual invariants of families of coplanar conics. These invariants are compared with the use of invariants of two conics and a case is presented where the proposed invariants have a greater discriminating power than the previously used ... Keywords: Segre characteristics, conics, invariants, lambda matrices, object recognition

Douglas R. Heisterkamp; Prabir Bhattacharya

1997-06-01T23:59:59.000Z

253

Single Production of Fourth Family b' Quark at the Large Hadron electron Collider  

E-Print Network (OSTI)

We examined the single production of fourth family b' quarks at the Large Hadron electron Collider (LHeC).We have analyzed the background and the signal processes for the mass range 300-800 GeV. We find the discovery region for an optimal parametrization of the Vqb' matrix elements.

O. Cakir; V. Cetinkaya

2009-12-10T23:59:59.000Z

254

ORNL's alumina-forming austenitic alloy family licensed to Carpenter Technology Corp.  

E-Print Network (OSTI)

ORNL's alumina-forming austenitic alloy family licensed to Carpenter Technology Corp. Carpenter Technology Corp. has licensed an alumina-forming austenitic (AFA) stainless steel alloy developed under technology from the initial conception and alloy design stage to the first successful prototype alloy

255

One-parameter family of indecomposable optimal entanglement witnesses arising from generalized Choi maps  

SciTech Connect

In a recent paper [D. Chruscinski and F. A. Wudarski, Open Sys. Information Dyn. (unpublished)], it was conjectured that the entanglement witnesses arising from some generalized Choi maps are optimal. We show that this conjecture is true. Furthermore, we show that they provide a one-parameter family of indecomposable optimal entanglement witnesses.

Ha, Kil-Chan [Faculty of Mathematics and Statistics, Sejong University, Seoul 143-747 (Korea, Republic of); Kye, Seung-Hyeok [Department of Mathematics, Seoul National University, Seoul 151-742 (Korea, Republic of)

2011-08-15T23:59:59.000Z

256

Characterization of a new family of metal transport proteins. 1998 annual progress report  

Science Conference Proceedings (OSTI)

'Soils at many DOE sites are contaminated with metals and radionuclides. Such soils obviously pose a risk to human and animal health. Unlike organic wastes which can be metabolized, metals are immutable and cannot be degraded into harmless constituents. Phytoremediation, the use of plants to remove toxic materials from soil and water, may prove to be an environmentally friendly and cost effective solution for cleaning up metal-contaminated sites. The success of phytoremediation will rely on the availability of plants that absorb, translocate, and tolerate the contaminating metals. However, before the authors can engineer such plants, they need more basic information on how plants acquire metals. An important long term goal of the research program is to understand how metals such as zinc, cadmium and copper are transported across membranes. The research is focused on a new family of metal transporters which they have identified through combined studies in the yeast Saccharomyces cerevisiae and in the model plant Arabidopsis thaliana. They have identified a family of 19 presumptive metal transport genes in a variety of organisms including yeast, trypanosomes, plants, nematodes, and humans. This family, which the authors have designated the ZIP genes, provides a rich source of material with which to undertake studies on metal transport in eukaryotes. The project has three main objectives: Objective 1: Determine the sub-cellular location of the ZIP proteins in Arabidopsis. Objective 2: Carry out a structure/function analysis of the proteins encoded by the ZIP gene family to identify regions of the protein responsible for substrate specificity and affinity. Objective 3: Engineer plants to overexpress and underexpress members of the ZIP gene family and analyze these transgenic plants for alterations in metal accumulation. They now know that manipulation of transporter levels will also require an understanding of post-transcriptional control of ZIP gene expression. They are currently in year one of a three-year project.'

Guerinot, M.L.

1998-06-01T23:59:59.000Z

257

Complete genome sequences for the anaerobic, extremely thermophilic plant biomass-degrading bacteria Caldicellulosiruptor hydrothermalis, Caldicellulosiruptor kristjanssonii, Caldicellulosiruptor kronotskyensis, Caldicellulosiruptor owensenis, and Caldicellulosiruptor lactoaceticus  

Science Conference Proceedings (OSTI)

The genus Caldicellulosiruptor contains the most thermophilic, plant biomass-degrading bacteria isolated to date. Previously, genome sequences from three cellulolytic members of this genus were reported (C. saccharolyticus, C. bescii, and C. obsidiansis). To further explore the physiological and biochemical basis for polysaccharide degradation within this genus, five additional genomes were sequenced: C. hydrothermalis, C. kristjanssonii, C. kronotskyensis, C. lactoaceticus, and C. owensensis. Taken together, the seven completed and one draft-phase Caldicellulosiruptor genomes suggest that, while central metabolism is highly conserved, significant differences in glycoside hydrolase inventories and numbers of carbohydrate transporters exist, a finding which likely relates to variability observed in plant biomass degradation capacity.

Blumer-Schuette, Sara E. [North Carolina State University; Ozdemir, Inci [North Carolina State University; Mistry, Dhaval [North Carolina State University; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Walston Davenport, Karen [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Adams, Michael W. W. [University of Georgia, Athens, GA; Kelly, Robert M [North Carolina State University

2011-01-01T23:59:59.000Z

258

The role of the school social worker in family involvement as identified by family specialists and parents in selected title I schools in North East Independent School District in San Antonio, Texas  

E-Print Network (OSTI)

This study examined the influence of social workers placed at the elementary school level, who work with low-socioeconomic families. The intent of the study was to examine the role of the social worker, at identified Title I elementary schools, on parent involvement. Research Question 1 asked, "What influence do the family specialists have in family involvement as reported by parents and family specialists at the identified Title I elementary schools in the North East ISD, in San Antonio, Texas?" The results of this study strongly support that the family specialist on the campus did have a positive influence on parents getting involved at their child's school. Research Question 2 asked, "What selected variables influence parental involvement as reported by parents and family specialists, who are participants of the Parent Academy at the selected Title I elementary schools in the North East ISD, in San Antonio, Texas?" The results of this study strongly supported that the variables of open communication between home and school and the school being inviting to parents are positive factors in getting parents involved. Research Question 3 asked, "What influence do community agencies have in assisting with the needs of families, as reported on the end-of-year summary sheets by the family specialists at the selected Title I elementary schools in the North East ISD, in San Antonio, Texas?" The results of this study showed that data procedures were inconsistent among the family specialists in the North East ISD. Data concerning the use of social service agencies was incomplete. No standard procedures are in place for tracking services provided to families. Research Question 4 asked, "What influence do parents have on determining the classes set forth for the Parent Academy, as reported by the parents and family specialists who are participants of the Parent Academy at the selected Title I elementary campuses in the North East ISD in San Antonio, Texas?" The results of this study strongly suggested that both parents and family specialists feel the parents have a voice in choosing the classes provided for them by the family specialists.

Canada, Gloria Lou

2007-12-01T23:59:59.000Z

259

The role of the school social worker in family involvement as identified by family specialists and parents in selected title 1 schools in north east independent school district in san antonio, texas  

E-Print Network (OSTI)

This study examined the influence of social workers placed at the elementary school level, who work with low-socioeconomic families. The intent of the study was to examine the role of the social worker, at identified Title I elementary schools, on parent involvement. Research Question 1 asked, What influence do the family specialists have in family involvement as reported by parents and family specialists at the identified Title I elementary schools in the North East ISD, in San Antonio, Texas? The results of this study strongly support that the family specialist on the campus did have a positive influence on parents getting involved at their childs school. Research Question 2 asked, What selected variables influence parental involvement as reported by parents and family specialists, who are participants of the Parent Academy at the selected Title I elementary schools in the North East ISD, in San Antonio, Texas? The results of this study strongly supported that the variables of open communication between home and school and the school being inviting to parents are positive factors in getting parents involved. Research Question 3 asked, What influence do community agencies have in assisting with the needs of families, as reported on the end-of-year summary sheets by the family specialists at the selected Title I elementary schools in the North East ISD, in San Antonio, Texas? The results of this study showed that data procedures were inconsistent among the family specialists in the North East ISD. Data concerning the use of social service agencies was incomplete. No standard procedures are in place for tracking services provided to families. Research Question 4 asked, What influence do parents have on determining the classes set forth for the Parent Academy, as reported by the parents and family specialists who are participants of the Parent Academy at the selected Title I elementary campuses in the North East ISD in San Antonio, Texas? The results of this study strongly suggested that both parents and family specialists feel the parents have a voice in choosing the classes provided for them by the family specialists.

Canada, Gloria Lou

2007-12-01T23:59:59.000Z

260

Family Structure, Marital Fertility and Premarital Sex among Married and Never-Married Women in Contemporary China  

E-Print Network (OSTI)

According to Chinese traditions, patrilocal residence is believed to be linked with early and high marital fertility. However, despite the rapid fertility decline and the enormous social and economic changes that have occurred in recent years in China, research still shows that family structure in China is relatively stable compared to western countries. This dissertation investigates the effects of family structure on fertility in contemporary China. This dissertation had two main objectives: first, to examine the effects of family structure on the marital fertility of married women; and second, to better understand the effects of family structure on the premarital fertility by examining the effects of family structure on premarital sex of never-married women. This dissertation utilizes data from the China Health and Nutrition Survey and the Chinese Health and Family Life Survey. Logistic regression model and Cox proportional hazards model are used to to estimate the the effects of family structure on marital fertility and premarital sex. The major finding in this dissertation shows that patrilocal residence has been well preserved in contemporary china. After controlling for relevant factors, co-residence or quasi-coresidence with parents-in-law significantly accelerates the transition from marriage to first birth, and promotes a desire for more children. However, second births are significantly impacted by factors associated with socioeconomic status and family planning policy other than family structure. This dissertation also confirmed the effects of family structure on premarital sex in terms of behavior, but not in terms of attitudes. After controlling for relevant factors, co-residence with parents significantly decreased the odds of engaging in premarital sex.

He, Lei 1984-

2012-12-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Family Characteristics  

Science Conference Proceedings (OSTI)

Table 6   Comparative properties of 30% glass reinforced electrical-grade plastics...7.6 (1.1) 9.7 (1.4) 5.5 (0.8) 11.7 (1.7) High volt track rate 0.0 7.8 0.8 0.3 7.1 High amp ignition >300 15 77 >200 >200...

262

Family Characteristics  

Science Conference Proceedings (OSTI)

Table 22   Chemical resistance of unreinforced PAEK E-1000 polymer...93 Isopropanol at 100 °C (212 °F) 98 90 143 129 Jet fuel A at 130 °C (265 °F) 98 100 136 57 Skydrol LD-4 at 130 °C (265 °F) 93 96 86 107 Deionized water at 130 °C (265 °F) 97 94 136 57 28% ammonium hydroxide at 60 °C (140 °F) 89 96 139 129 30% sodium hydroxide at 130 °C (265 °F) 96 96 107 86 20%...

263

Can the matter-antimatter asymmetry be easier to understand within the "spin-charge-family-theory", predicting twice four families and two times $SU(2)$ vector gauge and scalar fields?  

E-Print Network (OSTI)

This contribution is an attempt to try to understand the matter-antimatter asymmetry in the universe within the {\\it spin-charge-family-theory} if assuming that transitions in non equilibrium processes among instanton vacua and complex phases in mixing matrices are the sources of the matter-antimatter asymmetry, as studied in the literature for several proposed theories. The {\\it spin-charge-family-theory} is, namely, very promising in showing the right way beyond the {\\it standard model}. It predicts families and their mass matrices, explaining the origin of the charges and of the gauge fields. It predicts that there are, after the universe passes through two $SU(2)\\times U(1)$ phase transitions, in which the symmetry breaks from $SO(1,3) \\times SU(2) \\times SU(2) \\times U(1) \\times SU(3)$ first to $SO(1,3) \\times SU(2) \\times U(1) \\times SU(3)$ and then to $SO(1,3) \\times U(1) \\times SU(3)$, twice decoupled four families. The upper four families gain masses in the first phase transition, while the second four families gain masses at the electroweak break. To these two breaks of symmetries the scalar non Abelian fields, the (superposition of the) gauge fields of the operators generating families, contribute. The lightest of the upper four families is stable (in comparison with the life of the universe) and is therefore a candidate for constituting the dark matter. The heaviest of the lower four families should be seen at the LHC or at somewhat higher energies.

N. S. Mankoc Borstnik

2010-11-26T23:59:59.000Z

264

Abnormal Gene Expression of Four Genes in Cells from Family Members of  

NLE Websites -- All DOE Office Websites (Extended Search)

Abnormal Gene Expression of Four Genes in Cells from Family Members of Abnormal Gene Expression of Four Genes in Cells from Family Members of Hereditary-type Retinoblastoma Patients relative to Normal Individuals Chin-Yu Wang, 1 Yuanlin Peng, 1 Zhonghui Yang, 2 Chuan-Yuan Li, 2 Hatsumi Nagasawa, 1 Markus M. Fitzek, 3 John B. Little, 4 Joel S. Bedford, 1 and Eric Y. Chuang 5 1 Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado; 2 Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina; 3 Department of Radiation Oncology, Tufts-New England Medical Center; 4 Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts; and 5 Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan

265

The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction  

NLE Websites -- All DOE Office Websites (Extended Search)

534 534 doi:10.1107/S0907444912050512 Acta Cryst. (2013). D69, 534-539 Acta Crystallographica Section D Biological Crystallography ISSN 0907-4449 The structure and mode of action of Caldicellulosiruptor bescii family 3 pectate lyase in biomass deconstruction Markus Alahuhta, a Roman Brunecky, a Puja Chandrayan, b Irina Kataeva, b Michael W. W. Adams, b Michael E. Himmel a and Vladimir V. Lunin a * a Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401-3305, USA, and b Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602-7229, USA Correspondence e-mail: vladimir.lunin@nrel.gov # 2013 International Union of Crystallography Printed in Singapore - all rights reserved The unique active site of the Caldicellulosiruptor bescii family 3 pectate lyase catalytic module (PL3-cat) has been structu-

266

Recommendations for 15% Above-Code Energy Efficiency Measures for Single-Family Residences  

E-Print Network (OSTI)

This paper presents an overview of the recommendations for achieving 15% above-code energy performance for single-family residences. The analysis was performed using a simulation model of an International Energy Conservation Code (IECC)- compliant, single family residence in Houston, Texas. To accomplish the 15% annual energy use reductions, twelve measures were considered, which include: tankless water heater, solar domestic hot water system, gas water heater without the standing pilot light, ducts in the conditioned space, improved duct sealing, increased air tightness, window shading and redistribution, improved window performance, improved heating and cooling system efficiency. After the total annual energy use was determined for each measure, they were then grouped to accomplish a 15% total annual energy use reduction.

Culp, C.; Haberl, J. S.; Mukhopadhyay, J.; Liu, J. B.; Yazdani, B.; Malhotra, M.

2007-12-01T23:59:59.000Z

267

A new look at the Plebanski-Demianski family of solutions  

E-Print Network (OSTI)

The Plebanski-Demianski metric, and those that can be obtained from it by taking coordinate transformations in certain limits, include the complete family of space-times of type D with an aligned electromagnetic field and a possibly non-zero cosmological constant. Starting with a new form of the line element which is better suited both for physical interpretation and for identifying different subfamilies, we review this entire family of solutions. Our metric for the expanding case explicitly includes two parameters which represent the acceleration of the sources and the twist of the repeated principal null congruences, the twist being directly related to both the angular velocity of the sources and their NUT-like properties. The non-expanding type D solutions are also identified. All special cases are derived in a simple and transparent way.

J. B. Griffiths; J. Podolsky

2005-11-16T23:59:59.000Z

268

The earth-coupled heat pump: Utilizing innovative technology in single family rehabilitation strategies  

DOE Green Energy (OSTI)

The study examines the feasibility of incorporating the use of earth-coupled heat pump technology in single-family housing rehabilitation projects, based on energy conservation attributes and financial considerations. Following evaluation of a theoretical model which indicated that installations of the heat pumps were feasible, the heat pumps were tested under actual conditions in five single family housing units which were part of the Urban Homesteading Program, and were matched with comparable units which did not receive special treatment. Energy consumption information was collected for all units for twelve months. Variables were identified, and the data was analyzed for individual housing units and compared with the results predicted by the theoretical model to determine the practicality of incorporating such technology in large scale rehabilitation projects. 14 refs., 14 figs., 3 tabs.

Not Available

1989-11-01T23:59:59.000Z

269

Solar energy heating system design package for a single-family residence at New Castle, Pennsylvania  

DOE Green Energy (OSTI)

The design of a solar heating and hot water system for the New Castle Redevelopment Authority's single-family dwelling located at New Castle, Pennsylvania is described. Documentation submitted by the contractor for Government review of plans, specifications, cost trade studies and verification status for approval to commit the system to fabrication is presented. Also included are system integration drawings, major subsystems drawings, and architect's specifications and plans.

Not Available

1977-08-01T23:59:59.000Z

270

A Transformation Method to Construct Family of Exactly Solvable Potentials in Quantum Mechanics  

E-Print Network (OSTI)

A transformation method is applied to the second order ordinary differential equation satisfied by orthogonal polynomials to construct a family of exactly solvable quantum systems in any arbitrary dimensional space. Using the properties of orthogonal polynomials, the method transforms polynomial differential equation to D-dimensional radial Schrodinger equation which facilitates construction of exactly solvable quantum systems. The method is also applied using associated Laguerre and Hypergeometric polynomials. The quantum systems generated from other polynomials are also briefly highlighted.

Nabaratna Bhagawati; N Saikia; N Nimai Singh

2013-08-26T23:59:59.000Z

271

The Home Energy Scoring Tool: A Simplified Asset Rating for Single Family  

NLE Websites -- All DOE Office Websites (Extended Search)

The Home Energy Scoring Tool: A Simplified Asset Rating for Single Family The Home Energy Scoring Tool: A Simplified Asset Rating for Single Family Homes Title The Home Energy Scoring Tool: A Simplified Asset Rating for Single Family Homes Publication Type Conference Proceedings LBNL Report Number LBNL-5714E Year of Publication 2012 Authors Bourassa, Norman, Leo I. Rainer, Evan Mills, and Joan Glickman Conference Name 2012 ACEEE Summer Study on Energy Efficiency in Buildings Date Published 05/2012 Conference Location Pacific Grove, CA, USA Abstract In 2010, the U.S. Department of Energy (DOE) and Lawrence Berkeley National Laboratory (LBNL) initiated development of a new web-based computer tool and method for providing an energy asset rating of single-family homes. The resulting Home Energy Scoring Tool (http://homeenergyscore.lbl.gov) is a key component of the DOE's Home Energy Score Program (http://www.homeenergyscore.gov) for residential building energy labeling, a voluntary national asset rating method that uses a simplified and standardized energy assessment process. The Scoring Tool component of the program has been designed to support the existing energy analysis marketplace by providing a substantially lower-cost entry-level assessment method. This paper presents technical details of the Home Energy Scoring Tool itself, including the Scoring Tool's relationship to the Home Energy Saver building simulation engine, the Home Energy Score calculation methodology, and the web services feature that allows any qualified third-party software developer to integrate the Home Energy Score method into their own webbased applications and market delivery strategy.

272

EPRI Family of Multi-Functional Low-Cost Solid-State Switchgear: Requirements Definition Phase  

Science Conference Proceedings (OSTI)

This report describes the findings of the research performed to assess the requirements for a next generation solid-state breakers ("all solid-state" as well as "hybrid" designs), identify the application areas, and evaluate the economic and technical considerations for different technologies and design options for a family of low-cost solid-state switchgears. The report outlines a research roadmap for design and development of the proposed technology and identifies the key functionalities and criteria t...

2005-12-22T23:59:59.000Z

273

Standard Work Specifications for Single-Family Home Energy Upgrades Summary (Fact Sheet)  

SciTech Connect

The U.S. Department of Energy (DOE), the National Renewable Energy Laboratory (NREL) and numerous industry stakeholders developed the Standard Work Specifications for Single-Family Home Energy Upgrades to define the minimum requirements for high-quality residential energy upgrades. Today, the Standard Work Specifications provide a unique source for defining high-quality home energy upgrades, establishing clear expectations for homeowners, contractors, trainers, workers, program administrators, and organizations that provide financing for energy upgrades.

Not Available

2012-11-01T23:59:59.000Z

274

Quantum-confinement versus strain effects in the Zn(Cd)S(Se) family of superlattices  

Science Conference Proceedings (OSTI)

We have investigated the quantum-confinement (QC) and strain effects on the band structures of the Zn(Cd)S(Se) family of binary-compound superlattices (SLs); particularly those with common anions and with common cations. All the studied cases of SLs ... Keywords: 71.20.Nr, 71.55.Gs, 73.21.Fg, 73.61.Ga, II-VI semiconductors, Semiconductor compounds, Superlattices

Ihab M. Obaidat; Nacir Tit

2009-03-01T23:59:59.000Z

275

Linking Family Background and Home Language with English Reading Comprehension amog Bi/Multilinguals  

E-Print Network (OSTI)

The purpose of this study is to examine the links between family background and home language factors on English reading achievement among bi/multilingual students. To explore the potential predictors of English reading achievement among bi/multilinguals, the study included literacy related resource, family socio-economic status (SES), and immigration status as constructs for family background; while for home language factors, the study looked at the orthography of home language, language preference, and multilingualism. Additionally, the study assessed the roles of school level factors (i.e., low SES students and English language learners in school) on school reading performance. The International Association for Evaluation of Educational Achievements Progress in International Reading Literacy Study (IEA PIRLS, 2006) data of Singaporean fourth grade students were used. The data were potentially nested, therefore, the models formulated in this study had multilevel structures, student- and school-levels. The results indicated that number of childrens books at home was the strongest predictor among other family background variables including parental education. However, having childrens books in English had a very small influence on English reading scores of the bi/multilingual students. More interestingly, the study found that parental immigration status did not influence students English reading scores. Looking at home language factors, the study found that the orthography of home language linked to English reading. Importantly, the study found that Malay language, which shares the same orthography with English (i.e., alphabetic), showed the strongest link to the students English reading achievement. Other variables of home language did not significantly predict English reading after controlling for parental education.

Yulia, Astri

2013-05-01T23:59:59.000Z

276

Identifying Inefficient Single-Family Homes With Utility Bill Analysis: Preprint  

Science Conference Proceedings (OSTI)

Differentiating between energy-efficient and inefficient single-family homes on a community scale helps identify and prioritize candidates for energy-efficiency upgrades. Prescreening diagnostic procedures can further retrofit efforts by providing efficiency information before a site-visit is conducted. We applied the prescreening diagnostic to a simulated community of homes in Boulder, Colorado and analyzed energy consumption data to identify energy-inefficient homes.

Casey, S.; Krarti, M.; Bianchi, M.; Roberts, D.

2010-08-01T23:59:59.000Z

277

Maximum pseudolikelihood estimator for exponential family models of marked Gibbs point processes  

E-Print Network (OSTI)

This paper is devoted to the estimation of a vector $\\bm {\\theta}$ parametrizing an energy function of a Gibbs point process, via the maximum pseudolikelihood method. Strong consistency and asymptotic normality results of this estimator depending on a single realization are presented. In the framework of exponential family models, sufficient conditions are expressed in terms of the local energy function and are verified on a wide variety of examples.

Billiot, Jean-Michel; Drouilhet, Rmy

2008-01-01T23:59:59.000Z

278

Structure of GDNF Family Ligand Artemin Complexed with Its GFRa3 Receptor  

NLE Websites -- All DOE Office Websites (Extended Search)

GDNF Family Ligand Artemin GDNF Family Ligand Artemin Complexed with Its GFRa3 Receptor The glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN) are GDNF family ligands (GFLs) that are crucial for the development and maintenance of many neurons [1,2]. The trophic effect of GFLs on the dopamine and motor neurons has stimulated interest in their use for the treatment of neurodegenerative diseases such as Parkinson's. These structurally related neurotrophic factors signal by forming a ternary complex with a nonsignaling, ligand-specific GFRa receptor and a signaling and shared receptor tyrosine kinase RET. Four different GFRa receptors (GFRa1-4) have been identified. The preferential interactions between GFLs and GFRa receptors have also been established as GDNF to GFRa1, NRTN to GFRa2, ARTN to GFRa3, and PSPN to GFRa4 [3]. Given the importance of GFLs in basic neurobiology and their potential therapeutic value, it is a compelling goal to understand the molecular basis of the interactions between GFLs and their receptors.

279

Hot Water Draw Patterns in Single-Family Houses: Findings from Field  

NLE Websites -- All DOE Office Websites (Extended Search)

Hot Water Draw Patterns in Single-Family Houses: Findings from Field Hot Water Draw Patterns in Single-Family Houses: Findings from Field Studies Title Hot Water Draw Patterns in Single-Family Houses: Findings from Field Studies Publication Type Report LBNL Report Number LBNL-4830E Year of Publication 2011 Authors Lutz, James D., Renaldi, Alexander B. Lekov, Yining Qin, and Moya Melody Document Number LBNL-4830E Pagination 26 Date Published 05/2011 Publisher Lawrence Berkeley National Laboratory City Berkeley Abstract This report describes data regarding hot water draw patterns that Lawrence Berkeley National Laboratory obtained from 10 studies. The report describes our purposes in collecting the data; the ways in which we managed, cleaned, and analyzed the data; and the results of our data analysis. We found that daily hot water use is highly variable both among residences and within the same residence. We also found that the distributions of daily hot water use are not symmetrical normal distributions. Thus we used median, not average, values to characterize typical daily hot water use. This report presents summary information that illustrates the results of our data collection and some initial analysis.

280

Using family history information to promote healthy lifestyles and prevent diseases;a discussion of the evidence  

E-Print Network (OSTI)

information. Phy- sicians have reported time restrictions, lack of reimburse- ment, and the complexity of familial risk interpretation as barriers to the routine and systematic collection and use of family history for disease prevention [68]. Decision support... :248 http://www.biomedcentral.com/1471-2458/10/248 Page 6 of 7 30. Valdez R, Yoon PW, Qureshi N, Green RF, Khoury MJ: Family history in public health practice; a genomic tool for disease prevention and health promotion. Annu Rev Public Health 2010, 31...

Claassen, Liesbeth; Henneman, Lidewij; Janssens, A Cecile J W; Wijdenes-Pijl, Miranda; Qureshi, Nadeem; Walter, Fiona M; Yoon, Paula W; Timmermans, Danielle R M

2010-05-13T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

ORP-3 Rescues ER Membrane Expansions Caused by the VAPB-P56S Mutation in Familial ALS .  

E-Print Network (OSTI)

??A mutation in ER membrane protein VAPB is responsible for causing a familial form of ALS (ALS8). The VAPB-P56S mutation causes protein aggregation and a (more)

Darbyson, Angie L.

2013-01-01T23:59:59.000Z

282

Characteristics, Welfare Use and Material Hardship Among California AFDC Households with Disabled and Chronically Ill Family Members  

E-Print Network (OSTI)

Families with Severely Disabled Members, 262 cases weightedA F D C Households with Disabled and Chronically 111 Familylevels. 1'he treatment o f disabled individuals in these

Meyers, Marcia k.

1996-01-01T23:59:59.000Z

283

Time Running Out: A Portrait of California Families Reaching the CalWORKs Time Limit in 2004, Detailed Research Findings  

E-Print Network (OSTI)

reported being sick or disabled, one-third knew that suchnot report being sick or disabled understood this to be theold age, disability, caring for a disabled family member, or

London, Rebecca A.; Mauldon, Jane G.

2006-01-01T23:59:59.000Z

284

PU-BCD: exponential family models for the coarse- and fine-grained all-words tasks  

Science Conference Proceedings (OSTI)

This paper describes an exponential family model of word sense which captures both occurrences and co-occurrences of words and senses in a joint probability distribution. This statistical framework lends itself to the task of word sense disambiguation. ...

Jonathan Chang; Miroslav Dudk; David M. Blei

2007-06-01T23:59:59.000Z

285

The Massachusetts HomeBASE Program : lessons learned from a statewide housing-first policy for homeless families  

E-Print Network (OSTI)

Massachusetts is the only state in the US to maintain an emergency shelter entitlement for homeless families with its own dedicated line item in the state budget. However, in the last decade that line item has increased ...

Ward, Ellen Elizabeth

2012-01-01T23:59:59.000Z

286

Unconventional Ubiquitin Recognition by the Ubiquitin-Binding Motif within the Y-Family DNA Polymerases ? and Rev1  

E-Print Network (OSTI)

Translesion synthesis is an essential cell survival strategy to promote replication after DNA damage. The accumulation of Y family polymerases (pol) ? and Rev1 at the stalled replication machinery is mediated by the ...

DSouza, Sanjay

287

Incremental densification auctions : A politically viable method of producing infill housing in existing single-family neighborhoods  

E-Print Network (OSTI)

This paper examines the problem of convincing homeowners to accept new housing density in their neighborhoods. This paper proposes that densification that places additional housing units in preexisting single-family ...

Baker, Karl Phillip

2008-01-01T23:59:59.000Z

288

Transcriptional regulation of antimicrobial peptide induction by NF-[kappa]B family members during the Drosophila melanogaster immune response  

E-Print Network (OSTI)

Dl), a member of the NF-kB family, through degradation ofworks in conjunction with NF-kB members to regulate fat bodystandard deviation. (C). Role of fly NF-kB proteins in AttA

Busse, Matthew Schmidt

2007-01-01T23:59:59.000Z

289

Tacticity control of polypropylene using a C2-symmetric family of catalysts  

E-Print Network (OSTI)

A family of C2-symmetric catalysts was designed and synthesized with the intent to polymerize propylene. The catalyst was designed to be C2-symmetric for the specific goal that the catalyst would have two identical sites for the propagation of the polymer and therefore eliminate some of the stereoerrors that occur in the propagation of the polymer chain. This catalyst would also operate under simple enantiomorphic site control and therefore the insertion of the monomer would be governed by the ligand surrounding the active site. The ligands were synthesized with increasing degrees of steric bulk with the intention to determine if a catalyst system could generate elastomeric polypropylene. Enantiomorphic site control polypropylene utilizes statistical methods to determine the Si and Re content of a given polymer chain as a function of the variable E. Polypropylene samples generated by the catalyst family were analyzed using high temperature pentad analysis of the methyl region to calculate the E value. The goal was to observe E to be equal to 0.78 provided that the number molecular weight of the polymer was 100,000. The catalyst systems generated polymers with values higher and lower than the desired 0.78, indicating too high or too low enantioselectivity of the catalyst systems respectively at Tp = 0C.

Rife, Nathan Prentice

2007-12-01T23:59:59.000Z

290

Tacticity control of polypropylene using a C2-symmetric family of catalysts  

E-Print Network (OSTI)

A family of C2-symmetric catalysts was designed and synthesized with the intent to polymerize propylene. The catalyst was designed to be C2-symmetric for the specific goal that the catalyst would have two identical sites for the propagation of the polymer and therefore eliminate some of the stereoerrors that occur in the propagation of the polymer chain. This catalyst would also operate under simple enantiomorphic site control and therefore the insertion of the monomer would be governed by the ligand surrounding the active site. The ligands were synthesized with increasing degrees of steric bulk with the intention to determine if a catalyst system could generate elastomeric polypropylene. Enantiomorphic site control polypropylene utilizes statistical methods to determine the Si and Re content of a given polymer chain as a function of the variable E. Polypropylene samples generated by the catalyst family were analyzed using high temperature pentad analysis of the methyl region to calculate the E value. The goal was to observe E to be equal to 0.78 provided that the number molecular weight of the polymer was 100,000. The catalyst systems generated polymers with values higher and lower than the desired 0.78, indicating too high or too low enantioselectivity of the catalyst systems respectively at Tp = 0C.

Rife, Nathan Prentice

2007-12-01T23:59:59.000Z

291

Family of Finite Geometry Low-Density Parity-Check Codes for Quantum Key Expansion  

E-Print Network (OSTI)

We consider a quantum key expansion (QKE) protocol based on entanglement-assisted quantum error-correcting codes (EAQECCs). In these protocols, a seed of a previously shared secret key is used in the post-processing stage of a standard quantum key distribution protocol like the Bennett-Brassard 1984 protocol, in order to produce a larger secret key. This protocol was proposed by Luo and Devetak, but codes leading to good performance have not been investigated. We look into a family of EAQECCs generated by classical finite geometry (FG) low-density parity-check (LDPC) codes, for which very efficient iterative decoders exist. A critical observation is that almost all errors in the resulting secret key result from uncorrectable block errors that can be detected by an additional syndrome check and an additional sampling step. Bad blocks can then be discarded. We make some changes to the original protocol to avoid the consumption of the preshared key when the protocol fails. This allows us to greatly reduce the bit error rate of the key at the cost of a minor reduction in the key production rate, but without increasing the consumption rate of the preshared key. We present numerical simulations for the family of FG LDPC codes, and show that this improved QKE protocol has a good net key production rate even at relatively high error rates, for appropriate choices of these codes.

Kung-Chuan Hsu; Todd A. Brun

2013-03-07T23:59:59.000Z

292

Making It Happen: Achieving Energy Efficiency in Multi-Family Buildings Housing Low-Income Tenants  

E-Print Network (OSTI)

Saving energy in multi-family buildings is a comparatively easy task to accomplish in theory: engineering science has shown us how to reduce heatloss and air infiltration, how to balance systems and improve heating plant efficiency, and how to capture warmth from the air, the earth and the sea. But getting this knowledge into multi-family buildings and making them energy efficient in fact is very difficult, especially if those buildings house low-income and elderly tenants, the people for whom saving energy is most urgent. Energy practitioners have found that multifamily building owners are not buying energy efficiency because it is not being marketed intelligently; affordable financing is very difficult to obtain, and energy education tailored to the needs of owners, occupants and maintenance crews is practically unknown. This paper discusses how four non-profit energy companies, located in major cities, overcame these obstacles. It explains how they market energy conservation improvements, how they finance them, and how they involve tenants in energy education; i.e., how they make energy efficiency happen in multifamily buildings.

Haun, C. R.

1985-01-01T23:59:59.000Z

293

Economics of Condensing Gas Furnaces and Water Heaters Potential in Residential Single Family Homes  

SciTech Connect

Residential space and water heating accounts for over 90percent of total residential primary gas consumption in the United States. Condensing space and water heating equipment are 10-30percent more energy-efficient than conventional space and water heating. Currently, condensing gas furnaces represent 40 percent of shipments and are common in the Northern U.S. market. Meanwhile, manufacturers are planning to develop condensing gas storage water heaters to qualify for Energy Star? certification. Consumers, installers, and builders who make decisions about installing space and water heating equipment generally do not perform an analysis to assess the economic impacts of different combinations and efficiencies of space and water heating equipment. Thus, equipment is often installed without taking into consideration the potential life-cycle economic and energy savings of installing space and water heating equipment combinations. Drawing on previous and current analysis conducted for the United States Department of Energy rulemaking on amended standards for furnaces and water heaters, this paper evaluates the extent to which condensing equipment can provide life-cycle cost-effectiveness in a representative sample of single family American homes. The economic analyses indicate that significant energy savings and consumer benefits may result from large-scale introduction of condensing water heaters combined with condensing furnaces in U.S. residential single-family housing, particularly in the Northern region. The analyses also shows that important benefits may be overlooked when policy analysts evaluate the impact of space and water heating equipment separately.

Lekov, Alex; Franco, Victor; Meyers, Steve

2010-05-14T23:59:59.000Z

294

Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis  

Science Conference Proceedings (OSTI)

Glycosyltransferase family14 (GT14) belongs to the glycosyltransferase (GT) superfamily that plays important roles in the biosynthesis of cell walls, the most abundant source of cellulosic biomass for bioethanol production. It has been hypothesized that DUF266 proteins are a new class of GTs related to GT14. In this study, we identified 62 GT14 and 106 DUF266 genes (named GT14-like herein) in Arabidopsis, Oryza, Populus, Sorghum and Vitis. Our phylogenetic analysis separated GT14 and GT14-like genes into two distinct clades, which were further divided into eight and five groups, respectively. Similarities in protein domain, 3D structure and gene expression were uncovered between the two phylogenetic clades, supporting the hypothesis that GT14 and GT14-like genes belong to one family. Therefore, we proposed a new family name, GT14/GT14-like family that combines both subfamilies. Variation in gene expression and protein subcellular localization within the GT14-like subfamily were greater than those within the GT14 subfamily. One-half of the Arabidopsis and Populus GT14/GT14-like genes were found to be preferentially expressed in stem/xylem, indicating that they are likely involved in cell wall biosynthesis. This study provided new insights into the evolution and functional diversification of the GT14/GT14-like family genes.

Ye, Chuyu [ORNL; Li, Ting [ORNL; Tuskan, Gerald A [ORNL; Tschaplinski, Timothy J [ORNL; Yang, Xiaohan [ORNL

2011-01-01T23:59:59.000Z

295

An analysis of International Energy Conservation Code (IECC)-compliant single-family residential energy use  

E-Print Network (OSTI)

In 2001, the Texas State Senate passed Senate Bill 5 to reduce ozone levels by encouraging the reduction of emissions of NOx that were not regulated by the Texas Natural Resource Conservation Commission, including point sources (power plants), area sources (such as residential emissions), road mobile sources, and non-road mobile sources. For the building energy section, the Texas State Legislature adopted the 2000/2001 International Energy Conservation Code, as modified by the 2001 Supplement, as the state's building energy code. The 2000/2001 IECC is a comprehensive energy conservation code that establishes a standard for the insulation levels, glazing and cooling and heating system efficiencies through the use of prescriptive and performance-based provisions. Therefore, the purpose of this research is to improve the accuracy of a 2000/2001 IECCcompliant performance simulation using the DOE-2.1e simulation program to investigate the energy performance of a typical single-family house. To achieve this purpose, several objectives had to be accomplished, including: 1) the development of an IECC-compliant simulation model, 2) the development and testing of specific improvements to the existing code-traceable model, 3) the calibration and installation of sensors in a case-study house, 4) the validation of the improved simulation model with measured data from the case-study house, and 5) use the validated model to simulate the energy-conserving features of single-family residences that cannot be simulated with existing versions of the DOE-2.1e program. In order to create the code-traceable IECC-compliant simulation model, a base-case house simulation was created and the results calibrated with measured energy and environmental data from the case-study house. This was done in order to obtain an improved simulation model that would more accurately represent the case-study building. The calibrated model was then used to verify the accuracy of the improved simulation methods against previous models and measured data. After validation of the new simulation methodologies, the IECC simulation model was used to simulate different energy-conserving features for a single-family residence that could not be simulated with the previous version of the DOE-2 input file. Finally, areas for future work were identified in an effort to continue to improve the model.

Kim, Seongchan

2006-08-01T23:59:59.000Z

296

New Family of Tiny Crystals Glow Bright in LED Lights | Advanced Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

How Serotonin Receptors Can Shape Drug Effects, from LSD to Migraine How Serotonin Receptors Can Shape Drug Effects, from LSD to Migraine Medication X-rays Paint a Picture of Picasso's Pigments Antibody Evolution Could Guide HIV Vaccine Development Blue Ruthenium Dimer Catalysis for Hydrogen Generation A High-Pressure Nano-imaging Breakthrough Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed New Family of Tiny Crystals Glow Bright in LED Lights JUNE 7, 2013 Bookmark and Share Laue microdiffraction pattern acquired from a nanoribbon in experiments at the APS. Minuscule crystals that glow different colors may be the missing ingredient for white light-emitting diode (LED) lighting that illuminates homes and

297

T2SOLV: An enhanced package of solvers for the TOUGH2 family of codes  

DOE Green Energy (OSTI)

T2SOLV is an enhanced package of matrix solvers for the TOUGH2 family of codes.T2SOLV includes all the Preconditioned Conjugate Gradient (PCG) solvers used in T2CG1, the current solver package, as well as LUBAND, a new direct solver, and DLUSTB, a PCG solver based on the BiCGSTAB method. Additionally, T2SOLV includes the D4 grid numbering scheme and two sets of preprocessors. Results from test problems indicate that LUBAND is faster, more reliable and requires less storage than MA28, the BiCGSTAB solver is superior to the other PCG methods in T2SOLV, and that the preprocessors improve the performance of the PCG solvers and allow the solution of previously intractable problems.

Moridis, G.J.; Pruess, K. [Lawrence Berkeley National Lab., CA (United States). Earth Sciences Div.

1997-02-01T23:59:59.000Z

298

Selected cost considerations for geothermal district heating in existing single-family residential areas  

DOE Green Energy (OSTI)

In the past, district heating (geothermal or conventionally fueled) has not been widely applied to the single-family residential sector. Low-heat load density is the commonly cited reason for this. Although it`s true that load density in these areas is much lower than for downtown business districts, other frequently overlooked factors may compensate for load density. In particular, costs for distribution system installation can be substantially lower in some residential areas due to a variety of factors. This reduced development cost may partially compensate for the reduced revenue resulting from low-load density. This report examines cost associated with the overall design of the system (direct or indirect system design), distribution piping installation, and customer branch lines. It concludes with a comparison of the costs for system development and the revenue from an example residential area.

Rafferty, K.

1996-06-01T23:59:59.000Z

299

EVALUATION OF FCDA FAMILY SHELTER, MARK I, FOR PROTECTION AGAINST NUCLEAR WEAPONS. Preliminary Report  

SciTech Connect

An underground reinforced-concrete family shelter designed for six persons was tested at three anticipated nuclear blast overpressures: 30, 48, and 65 psi. The structures were calculated to sustain a 30-psi long-duration overpressure. Postshot examination of all shelters lndicated there was little or no deflection in the reinforced-concrete members. Although the actual blast load was of short duration, the natural period for the structures was also shortp therefore it is felt that the structures would withstand similar overpressures from long-duration blast loadings. The average attenuation factor for gamma radiation varied from 3000 to 4500. Permanent damage was confined to the exposed portions of the ventilation pipes, which were bent to a nearly horizontal position. The steel-plate door at the 65-psi level was dished inward about 1 1/4 in., but it opened and closed easily. (authl

FitzSimons, N.

1957-08-01T23:59:59.000Z

300

in the Philippine Pacific Seaboard and Celebes Sea with Focus on Tuna Larvae (Family: Scombridae)  

E-Print Network (OSTI)

This study covers fish larval survey conducted by the Philippine multi-purpose vessel MV DA-BFAR in the Pacific seaboard and Celebes Sea in May to June 2010. The composition, distribution and abundance of fish eggs and fish larvae are described based on 40 sampling stations. There were 87 families identified and 8.8 % of the total samples were Scombridae (tunas and mackerels). Of the tunas and mackerels, 39% were yellowfin (Thunnus albacares),21 % skipjack (Katsuwonus pelamis), 15 % bigeye (Thunnus obesus) and 15 % mackerels. Majority of these species were under preflexion stage. Attempt was also made to compare the composition and distribution of tuna larvae according to four geographical areas(Batanes-Polilio Island, Catanduanes-Eastern Samar, Siargao-Davao, and Mindanao-Celebes Sea).

A. Dickson; L. Nepomuceno; R. Ramiscal; Rhoda Servidad-bacordo; Alma C. Dickson; Laureana T. Nepomuceno; Rafael V. Ramiscal

2012-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Installation guidelines for Solar Heating System, single-family residence at New Castle, Pennsylvania  

DOE Green Energy (OSTI)

The Solar Heating System installer guidelines are provided for each subsystem and includes testing and filling the system. This single-family residential heating system is a solar-assisted, hydronic-to-warm-air system with solar-assisted domestic water heating. It is composed of the following major components: liquid cooled flat plate collectors; water storage tank; passive solar-fired domestic water preheater; electric hot water heater; heat pump with electric backup; solar hot water coil unit; tube-and-shell heat exchanger, three pumps, and associated pipes and valving in an energy transport module; control system; and air-cooled heat purge unit. Information is also provided on the operating procedures, controls, caution requirements, and routine and schedule maintenance. Information consists of written procedures, schematics, detail drawings, pictures and manufacturer's component data.

Not Available

1980-01-01T23:59:59.000Z

302

Passive heating and cooling strategies for single family housing in Fresno, California: a case study  

E-Print Network (OSTI)

This study focuses on the integration of passive heating, cooling, and ventilating techniques for detached single family housing in Fresno, California. The energy use and patterns of energy use were simulated for a typical tract house in Fresno, and serves as a case study, to which energy saving strategies were applied and evaluated using Ener-Win software. The effectiveness of each strategy was assessed based on the annual savings, the initial cost, and a life-cycle cost analysis. Specific areas of evaluation include: shading, improving the R-value and infiltration rate of the building envelope, thermal mass, natural ventilation, and evaporative cooling. The optimum strategies selected utilize only traditional building techniques. Evaporative cooling used in conjunction with an air conditioner was the most effective energy reducing strategy, but a combination of purely passive strategies yield competitive results. Although the typical Fresno home is already energy efficient, small alterations provide energy savings up to 75% for space conditioning.

Winchester, Nathan James

1995-01-01T23:59:59.000Z

303

Yukawaon Model with Anomaly Free Set of Quarks and Leptons in a U(3) Family Symmetry  

E-Print Network (OSTI)

In the so-called "yukawaon" model, the (effective) Yukawa coupling constants $Y_f^{eff}$ are given by vacuum expectation values (VEVs) of scalars $Y_f$ (yukawaons) with $3\\times 3$ components. So far, yukawaons $Y_f$ have been assigned to ${\\bf 6}$ or ${\\bf 6}^*$ of U(3) family symmetry, so that quarks and leptons were not anomaly free in U(3). In this paper, yukawaons are assigned to ${\\bf 8}+{\\bf 1}$ of U(3), so that quarks and leptons are anomaly free. Since VEV relations among yukawaons are also considerably changed, parameter fitting of the model is renewed. After fixing our free parameters by observed mass ratios, we have only two and one remaining free parameters for quark and lepton mixings, respectively. We obtain successful predictions for the quark and lepton mixing parameters including magnitudes of $CP$ violation. The effective Majorana neutrino mass is also predicted.

Yoshio Koide; Hiroyuki Nishiura

2013-08-09T23:59:59.000Z

304

Measured energy savings and economics of retrofitting existing single-family homes: An update of the BECA-B database  

SciTech Connect

The energy bill for US single-family households was over $77 billion in 1987 (excluding auto fuel purchases), accounting for approximately 20% of national energy expenditures. Large sums are spent on residential retrofits by individual homeowners, government agencies, and utilities. As of late 1987, over 21 million households indicated that they had added at least one energy-saving measure in the previous two years, while a recent Electric Power Research Institute (EPRI) study estimated that nearly 15 million residential customers have participated in some kind of demand-side management (DSM) program. Given the level of continuing investments in residential energy efficiency, accurate estimates of savings from various conservation measures are increasingly necessary, especially as new technologies become more sophisticated and incremental efficiency gains more difficult to achieve. This report provides a comparative analysis of measured data on the performance and cost-effectiveness of energy-saving measures in existing single-family homes, based on information in the Buildings Energy-Use Compilation and Analysis (BECA) data base. The initial BECA report on measured data for single-family retrofits was completed seven years ago. In updating the single-family database, we have added 135 data points, representing over 33,000 houses, to the original database of 145 retrofit projects. The report is organized in two volumes. Volume 1 provides a summary of energy savings and costs of individual retrofit measures and strategies and results from federal/state low-income and utility weatherization programs. we also discuss measurement issues, predicted versus actual savings, trends in single-family retrofit programs, and implications for the next generation'' of cost-effective single-family retrofits. Volume 2 contains a written summary of each retrofit project and complete data tables. 87 refs., 20 figs., 16 tabs.

Cohen, S.D.; Goldman, C.A.; Harris, J.P.

1991-02-01T23:59:59.000Z

305

INTEREST RATES FOR STAFFORD AND PLUS LOANS IN THE DIRECT LOAN AND FEDERAL FAMILY EDUCATION LOAN PROGRAMS  

E-Print Network (OSTI)

INTEREST RATES FOR STAFFORD AND PLUS LOANS IN THE DIRECT LOAN AND FEDERAL FAMILY EDUCATION LOAN First Disbursed Between July 1, 1998 and June 30, 2006 The variable interest rates for older Stafford to June 30, 2011 For the Period July 1, 2011 to June 30, 2012 Stafford Subsidized and Unsubsidized Loans

Chinnam, Ratna Babu

306

Family factors in Internet addiction among Chinese youth: A review of English- and Chinese-language studies  

Science Conference Proceedings (OSTI)

Approximately 513 million Chinese citizens used the Internet in 2011, with adolescents reporting comparatively high levels of use. Although numerous studies (reviewed herein) indicate that Internet Addiction/Pathological Internet Use (IA/PIU) is endemic ... Keywords: Chinese youth, Family factors, Internet addiction, Pathological Internet Use, Review article

Wen Li, Eric L. Garland, Matthew O. Howard

2014-02-01T23:59:59.000Z

307

Naming the pain in requirements engineering: design of a global family of surveys and first results from Germany  

Science Conference Proceedings (OSTI)

Context: For many years, we have observed industry struggling in defining a high quality requirements engineering (RE) and researchers trying to understand industrial expectations and problems. Although we are investigating the discipline with ... Keywords: family of studies, requirements engineering, survey research

Daniel Mndez Fernndez; Stefan Wagner

2013-04-01T23:59:59.000Z

308

Measured energy savings and economics of retrofitting existing single- family homes: An update of the BECA-B database  

SciTech Connect

These appendices are the companion volume to report number LBL--28147 Vol.1, with the same title. The summary data tables include physical characteristics, energy consumption, savings, and the retrofit measures installed and their costs for each retrofit project. Each existing single family residential building'' retrofit project in the BECA-B database is described. 99 refs. (BM)

Cohen, S.D.; Goldman, C.A.; Harris, J.P.

1991-02-01T23:59:59.000Z

309

Binding Preferences, Surface Attachment, Diffusivity, and Orientation of a Family 1 Carbohydrate-Binding Module on Cellulose  

DOE Green Energy (OSTI)

Cellulase enzymes often contain carbohydrate-binding modules (CBMs) for binding to cellulose. The mechanisms by which CBMs recognize specific surfaces of cellulose and aid in deconstruction are essential to understand cellulase action. The Family 1 CBM from the Trichoderma reesei Family 7 cellobiohydrolase, Cel7A, is known to selectively bind to hydrophobic surfaces of native cellulose. It is most commonly suggested that three aromatic residues identify the planar binding face of this CBM, but several recent studies have challenged this hypothesis. Here, we use molecular simulation to study the CBM binding orientation and affinity on hydrophilic and hydrophobic cellulose surfaces. Roughly 43 {mu}s of molecular dynamics simulations were conducted, which enables statistically significant observations. We quantify the fractions of the CBMs that detach from crystal surfaces or diffuse to other surfaces, the diffusivity along the hydrophobic surface, and the overall orientation of the CBM on both hydrophobic and hydrophilic faces. The simulations demonstrate that there is a thermodynamic driving force for the Cel7A CBM to bind preferentially to the hydrophobic surface of cellulose relative to hydrophilic surfaces. In addition, the simulations demonstrate that the CBM can diffuse from hydrophilic surfaces to the hydrophobic surface, whereas the reverse transition is not observed. Lastly, our simulations suggest that the flat faces of Family 1 CBMs are the preferred binding surfaces. These results enhance our understanding of how Family 1 CBMs interact with and recognize specific cellulose surfaces and provide insights into the initial events of cellulase adsorption and diffusion on cellulose.

Nimlos, M. R.; Beckham, G. T.; Matthews, J. F.; Bu, L.; Himmel, M. E.; Crowley, M. F.

2012-06-08T23:59:59.000Z

310

EFFECTS OF AT ATOMIC EXPLOSION ON GROUP AND FAMILY TYPE SHELTERS  

SciTech Connect

Two underground shelters (50-man capacity), one open and one closed, were exposed to Apple I shot, and two were exposed to Apple H shot (at 1050 ft). Three basement exit shelters were exposed to Apple I shot at 1350 ft; four were exposed to Apple II shot, two at 1270 ft, and two at 1470 ft. Groups of three aboveground utility type shelters, one of masonry blocks, one of precast reinforced concrete, and one of poured-in-place reinforced concrete, were placed at concrete bathroom shelters were placed in rambler type houses at 2700 and 10500 ft from Apple II shot. Three types of basement shelters were constructed in two frame houses at 5500 and 7800 ft, and two types of basement shelters were constructed in two brick houses at 4700 and 10500 ft from the same burst. On neither shot was structural damage sustained by the large underground personnel shelters. Occupants of the closed shelter would not have been disturbed by blast, debris, or radiation. Damage to the basement exit shelters was inversely proportional to their distance from Ground Zero (GZ) and was directly proportional to the amount of opening in the entrance. The closed shelter at the greatest distance received the least damage but was not satisfactory as a personnel shelter at the lowest pressure tested. Utility shelters provided unsatisfactory protection from radiation. All indoor family type shelters were satisfactory as tested and would have provided adequate protection for occupants. (auth)

Vortman, L.J.

1955-12-01T23:59:59.000Z

311

Energy Savings Resulting from Shading Devices on Single-Family Residences in Austin, Texas  

E-Print Network (OSTI)

Potential annual energy savings resulting from window shading devices on three prototypical Austin, Texas, single-family residences were computed in this study. Savings were calculated for interior (shades, blinds, draperies, window film, and tinted windows) and exterior (solar screens, awnings, overhangs, and the effects of recessed windows and vegetation) shading devices. The analysis was conducted with the DOE-2 building energy analysis computer program. Nominal baseline cases (single glazing, gas heating, and nominal shading from eaves and neighboring buildings) were run for each prototype. Selected baseline variants (double glazing, all electric, and no eaves or neighbor shading) were run to test parameter sensitivity. Results are reported in terms of the annual heating and cooling energy use and energy cost, with each device in place, as compared to the baseline cases. The devices are ranked in term of energy savings and energy coat savings. Another significant result is the multiple-regression correlation of annual heating and cooling energy savings with Shading Coefficient and U-value that generalizes the performance of the shading devices.

Pletzer, R. K.; Jones, J. W.; Hunn, B. D.

1987-01-01T23:59:59.000Z

312

The family of standard hydrogen monitoring system computer software design description: Revision 2  

DOE Green Energy (OSTI)

In March 1990, 23 waste tanks at the Hanford Nuclear Reservation were identified as having the potential for the buildup of gas to a flammable or explosive level. As a result of the potential for hydrogen gas buildup, a project was initiated to design a standard hydrogen monitoring system (SHMS) for use at any waste tank to analyze gas samples for hydrogen content. Since it was originally deployed three years ago, two variations of the original system have been developed: the SHMS-B and SHMS-C. All three are currently in operation at the tank farms and will be discussed in this document. To avoid confusion in this document, when a feature is common to all three of the SHMS variants, it will be referred to as ``The family of SHMS.`` When it is specific to only one or two, they will be identified. The purpose of this computer software design document is to provide the following: the computer software requirements specification that documents the essential requirements of the computer software and its external interfaces; the computer software design description; the computer software user documentation for using and maintaining the computer software and any dedicated hardware; and the requirements for computer software design verification and validation.

Bender, R.M.

1994-11-16T23:59:59.000Z

313

Applications of RESRAD family of computer codes to sites contaminated with radioactive residues.  

Science Conference Proceedings (OSTI)

The RESIL4D family of computer codes was developed to provide a scientifically defensible answer to the question ''How clean is clean?'' and to provide useful tools for evaluating human health risk at sites contaminated with radioactive residues. The RESRAD codes include (1) RESRAD for soil contaminated with radionuclides; (2) RESRAD-BUILD for buildings contaminated with radionuclides; (3) RESRAD-CHEM for soil contaminated with hazardous chemicals; (4) RESRAD-BASELINE for baseline risk assessment with measured media concentrations of both radionuclides and chemicals; (5) RESRAD-ECORISK for ecological risk assessment; (6) RESRAD-RECYCLE for recycle and reuse of radiologically contaminated metals and equipment; and (7) RESRAD-OFFSITE for off-site receptor radiological dose assessment. Four of these seven codes (RESRAD, RESRAD-BUILD, RESRAD-RECYCLE, and RESRAD-OFFSITE) also have uncertainty analysis capabilities that allow the user to input distributions of parameters. RESRAD has been widely used in the United States and abroad and approved by many federal and state agencies. Experience has shown that the RESRAD codes are useful tools for evaluating sites contaminated with radioactive residues. The use of RESRAD codes has resulted in significant savings in cleanup cost. Analysis of 19 site-specific uranium guidelines is discussed in the paper.

Yu, C.; Kamboj, S.; Cheng, J.-J.; LePoire, D.; Gnanapragasam, E.; Zielen, A.; Williams, W. A.; Wallo, A.; Peterson, H.

1999-10-21T23:59:59.000Z

314

Solar project description for Florida gas company's single family residence, Winter Springs, Florida  

DOE Green Energy (OSTI)

The Florida Gas Company solar energy system is installed in a 1548 square-foot, three bedroom single family dwelling located in Winter Springs, Florida. The system is designed to provide solar energy for space heating, space cooling, and domestic hot water heating. Solar energy is collected by two banks of double glazed flat plate collectors with a gross area of 714 square feet. Solar energy is transferred from the collector array to a 1350 gallon underground storage tank. Water is used as the heat collection, transfer and storage medium. Freeze protection is provided by means of circulation of hot water from storage through the collectors. No anti-freeze additive is required. A 3-ton solar energy powered absorption cycle Water Chiller provides chilled water for circulation through the same air distribution system. A gas fired boiler provides supplemental thermal energy to the chiller when sufficient thermal energy is not available from storage. Original cost estimates for provisioning and installation of the Solar System are given.

Not Available

1979-06-22T23:59:59.000Z

315

Structural Basis for NADH/NAD+ Redox Sensing by a Rex Family Repressor  

SciTech Connect

Nicotinamide adenine dinucleotides have emerged as key signals of the cellular redox state. Yet the structural basis for allosteric gene regulation by the ratio of reduced NADH to oxidized NAD{sup +} is poorly understood. A key sensor among Gram-positive bacteria, Rex represses alternative respiratory gene expression until a limited oxygen supply elevates the intracellular NADH:NAD{sup +} ratio. Here we investigate the molecular mechanism for NADH/NAD{sup +} sensing among Rex family members by determining structures of Thermus aquaticus Rex bound to (1) NAD{sup +}, (2) DNA operator, and (3) without ligand. Comparison with the Rex/NADH complex reveals that NADH releases Rex from the DNA site following a 40{sup o} closure between the dimeric subunits. Complementary site-directed mutagenesis experiments implicate highly conserved residues in NAD-responsive DNA-binding activity. These rare views of a redox sensor in action establish a means for slight differences in the nicotinamide charge, pucker, and orientation to signal the redox state of the cell.

McLaughlin, K.J.; Soares, A.; Strain-Damerell, C. M.; Xie, K.; Brekasis, D.; Pagent, M. S. B.; Kielkopf, C. L.

2010-05-28T23:59:59.000Z

316

HTGR Technology Family Assessment for a Range of Fuel Cycle Missions  

SciTech Connect

This report examines how the HTGR technology family can provide options for the once through, modified open cycle (MOC), or full recycle fuel cycle strategies. The HTGR can serve all the fuel cycle missions that an LWR can; both are thermal reactors. Additional analyses are warranted to determine if HTGR full recycle service could provide improved consumption of transuranic (TRU) material than LWRs (as expected), to analyze the unique proliferation resistance issues associated with the pebble bed approach, and to further test and analyze methods to separate TRISO-coated fuel particles from graphite and/or to separate used HTGR fuel meat from its TRISO coating. The feasibility of these two separation issues is not in doubt, but further R&D could clarify and reduce the cost and enable options not adequately explored at present. The analyses here and the now-demonstrated higher fuel burnup tests (after the illustrative designs studied here) should enable future MOC and full recycle HTGR concepts to more rapidly consume TRU, thereby offering waste management advantages. Interest in limited separation or minimum fuel treatment separation approaches motivates study of impurity-tolerant fuel fabrication.

Steven J. Piet; Samuel E. Bays; Nick R. Soelberg

2010-11-01T23:59:59.000Z

317

HTGR Technology Family Assessment for a Range of Fuel Cycle Missions  

SciTech Connect

This report examines how the HTGR technology family can provide options for the once through, modified open cycle (MOC), or full recycle fuel cycle strategies. The HTGR can serve all the fuel cycle missions that an LWR can; both are thermal reactors. Additional analyses are warranted to determine if HTGR full recycle service could provide improved consumption of transuranic (TRU) material than LWRs (as expected), to analyze the unique proliferation resistance issues associated with the pebble bed approach, and to further test and analyze methods to separate TRISO-coated fuel particles from graphite and/or to separate used HTGR fuel meat from its TRISO coating. The feasibility of these two separation issues is not in doubt, but further R&D could clarify and reduce the cost and enable options not adequately explored at present. The analyses here and the now-demonstrated higher fuel burnup tests (after the illustrative designs studied here) should enable future MOC and full recycle HTGR concepts to more rapidly consume TRU, thereby offering waste management advantages. Interest in limited separation or minimum fuel treatment separation approaches motivates study of impurity-tolerant fuel fabrication. Several issues are outside the scope of this report, including the following: thorium fuel cycles, gas-cooled fast reactors, the reliability of TRISO-coated particles (billions in a reactor), and how soon any new reactor or fuel type could be licensed and then deployed and therefore impact fuel cycle performance measures.

Steven J. Piet; Samuel E. Bays; Nick Soelberg

2010-08-01T23:59:59.000Z

318

Comparative ICE genomics: insights into the evolution of the SXT/R391 family of ICEs  

E-Print Network (OSTI)

Integrating and conjugative elements (ICEs) are one of the three principal types of self-transmissible mobile genetic elements in bacteria. ICEs, like plasmids, transfer via conjugation; but unlike plasmids and similar to many phages, these elements integrate into and replicate along with the host chromosome. Members of the SXT/R391 family of ICEs have been isolated from several species of gram-negative bacteria, including Vibrio cholerae, the cause of cholera, where they have been important vectors for disseminating genes conferring resistance to antibiotics. Here we developed a plasmid-based system to capture and isolate SXT/R391 ICEs for sequencing. Comparative analyses of the genomes of 13 SXT/R391 ICEs derived from diverse hosts and locations revealed that they contain 52 perfectly syntenic and nearly identical core genes that serve as a scaffold capable of mobilizing an array of variable DNA. Furthermore, selection pressure to maintain ICE mobility appears to have restricted insertions of variable DNA into intergenic sites that do not interrupt core functions. The variable genes confer diverse element-specific phenotypes, such as resistance to antibiotics. Functional analysis of a set of deletion mutants revealed that less than half of the conserved core genes are required for ICE mobility; the functions of most of the dispensable core genes are unknown. Several lines of evidence suggest that there has been extensive recombination between SXT/R391 ICEs, resulting in re-assortment of their respective variable gene content. Furthermore,

Rachel A. F. Wozniak; Derrick E. Fouts; Matteo Spagnoletti; Mauro M. Colombo

2009-01-01T23:59:59.000Z

319

Energy measurements of attic radiant barriers installed in single-family houses  

Science Conference Proceedings (OSTI)

Testing was conducted by the Oak Ridge National Laboratory to determine the energy savings attributable to radiant barriers installed in attics of unoccupied single-family houses. Three levels of fiberglass attic insulation (R-11 ,R-19, and R-30) were tested with two types of barrier installation (horizontal and truss). The results showed that horizontally installed radiant barriers were more effective than truss barriers in reducing heating and cooling loads. Measured cooling load reductions ranged form 0 to 22% (compared to same attic insulation insulation R-value with no radiant barrier) and heating load changes from /plus/4% to /minus/10% were measured (compared to same attic insulation R-value with no radiant barrier). Radiant barriers appeared to decrease the heating and cooling loads more when lesser amounts of insulation (R-11 and R-19) were present in an attic. Minimal changes were measured when R-30 was present in an attic. Long-term effects of dust on the performance of radiant barriers as well as the effects of moisture condensing on the surface of a radiant barrier during cold winter temperatures remain unanswered.

Levins, W.P.; Karnitz, M.A.

1988-07-01T23:59:59.000Z

320

Dynamic simulations of pathways downstream of ERBB-family: exploration of parameter space and effects of its variation on network behavior  

Science Conference Proceedings (OSTI)

The signaling-network immediately downstream of the ErbB-family is very important in BC and other cancers, especially considering treatment of the excess of function of dominant onco-proteins with oncoprotein inhibitors. We studied and implemented dynamic ... Keywords: ErbB-family, dynamic simulations, onco-protein inhibitors, oncogene mutations, parameter space, signaling-networks, systems biology

Lorenzo Tortolina; Nicoletta Castagnino; Cristina De Ambrosi; Raffaele Pesenti; Franco Patrone; Alberto Ballestrero; Eva Moran; Alessio Nencioni; Silvio Parodi

2010-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Variant Humicola grisea CBH1.1  

DOE Patents (OSTI)

Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

Goedegebuur, Frits (Vlaardingen, NL); Gualfetti, Peter (San Francisco, CA); Mitchinson, Colin (Half Moon Bay, CA); Larenas, Edmund (Moss Beach, CA)

2012-08-07T23:59:59.000Z

322

Variant Humicola grisea CBH1.1  

DOE Patents (OSTI)

Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

Goedegebuur, Frits (Vlaardingen, NL); Gualfetti, Peter (San Francisco, CA); Mitchinson, Colin (Half Moon Bay, CA); Larenas, Edmund (Moss Beach, CA)

2011-08-16T23:59:59.000Z

323

Variant Humicola grisea CBH1.1  

DOE Patents (OSTI)

Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.

Goedegeburr, Frits; Gualfetti, Peter; Mitchinson, Colin; Larenas, Edmund

2013-02-19T23:59:59.000Z

324

The Scattered Disk as the source of the Jupiter Family comets  

E-Print Network (OSTI)

The short period Jupiter family comets (JFCs) are thought to originate in the Kuiper Belt; specifically, a dynamical subclass of the Kuiper Belt known as the `scattered disk' is argued to be the dominant source of JFCs. However, the best estimates from observational surveys indicate that this source may fall short by more than two orders of magnitude the estimates obtained from theoretical models of the dynamical evolution of Kuiper belt objects into JFCs. We re-examine the scattered disk as a source of the JFCs and make a rigorous estimate of the discrepancy. We find that the uncertainties in the dynamical models combined with a change in the size distribution function of the scattered disk at faint magnitudes (small sizes) beyond the current observational limit offer a possible but problematic resolution to the discrepancy. We discuss several other possibilities: that the present population of JFCs is a large fluctuation above their long term average, that larger scattered disk objects tidally break-up into multiple fragments during close planetary encounters as their orbits evolve from the trans-Neptune zone to near Jupiter, or that there are alternative source populations that contribute significantly to the JFCs. Well-characterized observational investigations of the Centaurs, objects that are transitioning between the trans-Neptune Kuiper belt region and the inner solar system, can test the predictions of the non-steady state and the tidal break-up hypotheses. The classical and resonant classes of the Kuiper belt are worth re-consideration as significant additional or alternate sources of the JFCs.

Kathryn Volk; Renu Malhotra

2008-02-26T23:59:59.000Z

325

The mouse angiogenin gene family: Structures of an angiogenin-related protein gene and two pseudogenes  

SciTech Connect

Angiogenin, a homologue of pancreatic ribonuclease, is a potent inducer of blood vessel formation. As an initial step toward investigating the in vivo functional role of this protein via gene disruption, we undertook the isolation of the angiogenin gene (Ang) from the 129 strain mouse, which will be used for generating targeting constructs. Unexpectedly, screening of a genomic library with an Ang gene probe obtained previously from the BALB/c strain yielded two new genes closely similar to Ang rather than Ang itself. One of these encodes a protein with 78% sequence identity to angiogenin and is designated {open_quotes}Angrp{close_quotes} for {open_quotes}angiogenin-related protein.{close_quotes} The ribonucleolytic active site of angiogenin, which is critical for angiogenic activity, is completely conserved in Angrp, whereas a second essential site, thought to bind cellular receptors, is considerably different. Thus, the Angrp product may have a function distinct from that of angiogenin. The second gene obtained by library screening is a pseudogene, designated {open_quotes}Ang-ps1,{close_quotes} that contains a frame shift mutation in the early part of the coding region. Although the Ang gene was not isolated from this library, it was possible to amplify this gene from 129 mouse genomic DNA by the polymerase chain reaction (PCR). Sequence analysis showed that the 129 strain Ang gene is identical to the BALB/c gene throughout the coding region. PCR cloning also yielded a second Ang-like pseudogene, designated {open_quotes}Ang-ps2.{close_quotes} Southern blotting of genomic DNA confirmed the presence of Ang, Angrp, and at least one of the pseudogenes in an individual mouse and suggested that the mouse Ang gene family may contain more than the four members identified here. 31 refs., 4 figs., 1 tab.

Brown, W.E.; Nobile, V.; Shapiro, R. [Harvard Medical School, Boston, MA (United States)] [and others

1995-09-01T23:59:59.000Z

326

Analysis of photovoltaic total energy systems for single family residential applications  

DOE Green Energy (OSTI)

The performance and cost-effectiveness of three photovoltaic total energy system concepts designed to meet the thermal and electrical demands of a typical single family house are compared. The three photovoltaic total energy system concepts considered are: (1) All-photovoltaic systems. Passively air-cooled photovoltaic panels provide electricity to meet both electrical and thermal demands. (2) Separate-panel systems. Solar thermal panels provide thermal energy, while passively air-cooled photovoltaic panels serve the purely electric demand. (3) Combined thermal/electric panel systems. Water-cooled photovoltaic panels provide both thermal energy (transported by cooling water) and electrical energy to meet the separate thermal and electrical demands. Additional passively air-cooled photovoltaic panels are added, as required, to meet the electrical demand. The thermal demand is assumed to consist of the energy required for domestic hot water and space heating, while the electrical demand includes the energy required for baseload power (lights, appliances, etc.) plus air conditioning. An analysis procedure has been developed that permits definition of the panel area, electrical and/or thermal storage capacity, and utility backup energy level that, in combination, provide the lowest annual energy cost to the homeowner for each system concept for specified assumptions about costs and system operations. The procedure appears capable of being used to approximately any size system using solar collectors, as well as in any application where the thermal and/or electrical demand is being provided by solar energy, with utility or other conventional backup. This procedure has been used to provide results for homes located in Phoenix, Arizona, and Madison, Wisconsin, and to evaluate the effects of array and backup power costs and the desirability of selling excess electrical energy back to the utility. (WHK)

Chobotov, V.; Siegel, B.

1978-08-01T23:59:59.000Z

327

Cooling-energy measurements of unoccupied single-family houses with attics containing radiant barriers  

Science Conference Proceedings (OSTI)

Tests were conducted by Oak Ridge National Laboratory (ORNL) to determine the magnitude of the energy savings brought about by installing radiant barriers in the attics of single-family houses. The radiant barrier used for this test is a product with two reflective aluminum surfaces on a kraft paper base. The purpose of the radiant barrier is to reduce the radiant heat transfer component impinging on the fiberglass attic insulation. The radiant barrier works as a system in conjunction with an air space and can theoretically block up to 95% of far-infrared radiation heat transfer. The experiment was conducted in three unoccupied research houses that are operated by ORNL. Two variations on the installation of radiant barriers were studied. One house was used as the control house (no barrier was installed), while the other two were used to test the two different methods for installing the radiant barriers. In one house the barrier was laid on top of the attic fiberglass batt insulation, and in the other house, the barrier was attached to the underside of the roof trusses. The attics of all three houses were insulated with kraft-paper-faced R-19 fiberglass batt insulation. The results showed a savings in the cooling loads of 21% when the radiant barrier was laid on top of the attic fiberglass insulation and 13% with the radiant barrier attached to the underside of the roof trusses. The savings in electrical consumption were 17% and 9%, respectively. The electrical consumption data and the cooling load data indicate that the most effective way of installing the foil is to lay it on top of the fiberglass insulation. The radiant barriers reduced the measured peak ceiling heat fluxes by 39% for the case where the barrier was laid on top of the fiberglass insulation. The radiant barrier reduced the integrated heat flows from the attic to the house by approximately 30 to 35% over a 7-day time period.

Levins, W.P.; Karnitz, M.A.

1986-07-01T23:59:59.000Z

328

Interfacial inactivation of epoxide hydrolase in a two-liquid-phase system  

E-Print Network (OSTI)

at Midwest Research Institute (MRI). The concept is based on the use of the SpinCon® gas-liquid contactor the wall. Particles move outward toward the wall due to the centrifugal forces. Any particles that reach percent of the particles initially in the gas stream. Centrifugal forces resulted in the capture

Groningen, Rijksuniversiteit

329

The BAT1 gene in the MHC encodes an evolutionarily conserved putative nuclear RNA helicase of the DEAD family  

Science Conference Proceedings (OSTI)

The BAT1 gene has previously been identified about 30 kb upstream from the tumor necrosis factor (TNF) locus and close to a NF{sub kb}-related gene of the nuclear factor family in the major histocompatibility complex (MHC) of human, mouse, and pig. We now show that the BAT1 translation product is the homolog of the rat p47 nuclear protein, the WM6 Drosophila gene product, and probably also Ce08102 of Caenorhabditis elegans, all members of the DEAD protein family of ATP-dependent RNA helicases. This family has more than 40 members, including the eukaryotic translation initiation factor-4A (eIF-4A), the human nuclear protein p68, and the Drosophila oocyte polar granule component vasa. BAT1 spans about 10 kb, is split into 10 exons of varying length, and encodes a protein of 428 amino acids ({approximately}48 kDa). Human and pig BAT1 cDNAs display 95.6% identity in the coding region and 80% identity in the 5{prime} and 3{prime} noncoding regions. Several repeat sequences of different types were identified in introns of the porcine BAT1 gene. Three different mRNAs, 4.1,1.7, and 0.9 kb, respectively, were detected in all tissues analyzed upon hybridization with porcine BAT1 cDNA. Transfection and expression of human BAT1 cDNA after tagging with a heterologous antibody recognition epitope revealed a nuclear localization of the hybrid protein. An MspI RFLP was detected in an SLA class I typed family, confirming the localization of the BAT1 gene in the porcine MHC. BAT1 thus encodes a putative nuclear ATP-dependent RNA helicase and is likely to have an indispensable function. 35 refs., 6 figs., 1 tab.

Peelman, L.J.; Van Zeveren, A.; Coppeiters, W. [State Univ. Ghent, Merelbeke (Belgium)] [and others

1995-03-20T23:59:59.000Z

330

A Radiation Badge Survey for Family Members Living With Patients Treated With a {sup 103}Pd Permanent Breast Seed Implant  

SciTech Connect

Purpose: Sixty-seven patients with early-stage breast cancer were treated in a Phase I/II clinical trial using a {sup 103}Pd permanent breast seed implant as adjuvant radiotherapy after breast-conserving surgery. We report the dose received by family members living with these patients and compare measured doses with theoretical worst-case scenario estimates. Methods and Materials: Exposure-rate measurements were taken at 1 m from the patient by using a calibrated low-energy survey meter. Landauer (Landauer Inc., Glenwood, IL) Luxel badges, with sensitivity of 0.01 mSv, were given to family members to wear after the implantation. Badge readings for 33 spouses and 28 other family members were used to estimate effective doses, and these were compared with theory. Results: Average preimplantation planning target volume from computed tomography was 50.3 ml (range, 18.0-96.7 ml), and average preimplantation distance between the skin and the most anterior planning target volume margin was 0.57 cm. The average maximum exposure rate was measured to be 2.4 {+-} 1.1 mR/h, and average measured dose to a spouse was 0.99 {+-} 1.0 mSv. The calculated exposure rates and spousal doses using preimplantation computed tomography scan data overestimated those measured. Average measured family member dose (excluding spouses) was 0.20 {+-} 0.58 mSv. Conclusions: Based on measured and calculated spousal doses, a permanent breast seed implant using {sup 103}Pd is safe for the public. However, it is recommended that extra precautions in the way of a breast patch be used when patients with an implant will be in the vicinity of toddlers or pregnant women.

Keller, Brian M. [Department of Medical Physics, Sunnybrook Health Sciences Centre, Toronto, Ontario (Canada)], E-mail: brian.keller@sunnybrook.ca; Pignol, Jean-Philippe; Rakovitch, Eileen [Department of Radiation Oncology, Sunnybrook Health Sciences Centre, Toronto, Ontario (Canada); Sankreacha, Raxa; O'Brien, Peter [Department of Medical Physics, Sunnybrook Health Sciences Centre, Toronto, Ontario (Canada)

2008-01-01T23:59:59.000Z

331

Solar heating system design package for a single-family residence at William O'Brien State Park, Minnesota  

DOE Green Energy (OSTI)

Honeywell, Inc. has undertaken the design, fabrication, installation, and monitoring of a prototype solar heating and hot water system for the Minnesota Department of Natural Resources' single-family dwelling located at O'Brien State Park, 30 miles east of Minneapolis, Minnesota. Documentation submitted by Honeywell for government review of plans, specifications, cost trade studies and verification status in order to provide the contractor with approval to commit the system to fabrication are included.

Not Available

1977-07-01T23:59:59.000Z

332

Phylogeny and biogeography of the mayfly family Leptohyphidae (Insecta: Ephemeroptera) with a taxonomic revision of selected genera  

E-Print Network (OSTI)

A cladistic analysis of the world genera of the mayfly family Leptohyphidae is presented. Analyses of a matrix of 58 ingroup and 9 outgroup species and 119 morphological characters strongly supports the monophyly of Leptohyphidae and its sister-group relationship with Coryphoridae. Larval and adult taxonomic keys are provided to the 11 recognized extant genera. A synonymical listing, differential diagnosis, list of proposed synapomorphies, diagnostic illustrations, and notes on distribution and included species are given for each genus. The following new synonyms of genus Tricorythodes are proposed: Ableptemetes n. syn., Cabecar n. syn., Epiphrades n. syn., Homoleptohyphes n. syn., Macunahyphes n. syn., Tricoryhyphes n. syn. The former genus Asioplax is newly regarded as a subgenus of Tricorythodes. A species-level revision of North and Central American Leptohyphes is presented. A key to the 15 Leptohyphes species known as larvae is provided. In addition, detailed descriptions, diagnosis, and geographic distributions are given for all species of Leptohyphes known from North and Central America. Biogeographic analysis suggests that the family Leptohyphidae originated in South America, and that its North American species are the descendants of one or more ancestral species that crossed northward over the Panamanian land bridge. The results of this research clearly show that the mayfly family Leptohyphidae is a strongly supported monophyletic clade supported by five unique synapomorphies. Currently recognized genera are also strongly supported; however, little support was found for subfamilies. The sister family is clearly Coryphoridae, which is supported by three unique synapomorphies. Biogeographic analysis indicates that Leptohyphidae originated in South American, with at least five independent invasions from South America to North and Central America during the evolution of Leptohyphidae.

Baumgardner, David Eugene

2008-08-01T23:59:59.000Z

333

Modes of Action and Functions of ERECTA-family Receptor-like Kinases in Plant Organ Growth and Development  

DOE Green Energy (OSTI)

Higher plants constitute the central resource for renewable lignocellulose biomass that can supplement for the world's depleting stores of fossil fuels. As such, understanding the molecular and genetic mechanisms of plant organ growth will provide key knowledge and genetic resources that enables manipulation of plant biomass feedstock for better growth and productivity. The goal of this proposal is to understand how cell proliferation and growth are coordinated during aboveground organ morphogenesis, and how cell-cell signaling mediated by a family of receptor kinases coordinates plant organogenesis. The well-established model plant Arabidopsis thaliana is used for our research to facilitate rapid progress. Specifically, we focus on how ERECTA-family leucine-rich repeat receptor kinases (LRR-RLKs) interact in a synergistic manner to promote organogenesis and pattern formation in Arabidopsis. This project was highly successful, resulted in fourteen publications including nine peer-reviewed original research articles. One provisional US patent has been filed through this DOE funding. We have addressed the critical roles for a family of receptor kinases in coordinating proliferation and differentiation of plants, and we successfully elucidated the downstream targets of this signaling pathway in specifying stomatal patterning.

TORII, Keiko U.

2012-05-01T23:59:59.000Z

334

The Family of God: Universalism and Domesticity in Alice Cary's Fiction  

E-Print Network (OSTI)

Until recently Alice Cary's works have gone largely unnoticed by the literary community, and those critics who have examined her writings have recognized her primarily as a regionalist sketch writer. However, studying Cary's total body of fiction, including her novels and children's fiction as well as her sketches, and examining the influence of Christian Universalism upon her work reveals that Cary is a much more complex and nuanced writer than she has been previously understood to be. This dissertation explores the way that Cary questions stereotypes of accepted behavior specifically as they pertain to the identities of men, women, and children and offers a more flexible and inclusive religious identity rooted in Universalist ideals. In her depictions of women, Cary uses tropes from gothic stories, fairy tales, and sentimental fiction to criticize evangelical faith, Transcendentalism, and separate spheres-based stereotypes of women's behavior, and she undermines these stereotypes and replaces them with a Universalist emphasis on communal service and identity. Similarly, Cary's depictions of manhood are influenced by her desire to dissect preconceived notions of masculinity like that of the Self-Made Man and his earlier counterparts the Genteel Patriarch and the Heroic Artisan and replace these stereotypes with a Universalist model that embraces gender fluidity and sacrifice of self interest for the larger community. Cary's treatment of children continues her critique of nineteenth century stereotypes. Cary, unlike most early nineteenth century writers, exposes the dangers of romanticized visions of middle class children, which physically isolated children from their families and endangered working class children by increasing the demand for child labor; thus Cary's Universalism leads her to depict all children, not just the wealthy ones, as God's children and worthy of protection. Cary also uses children metaphorically to represent minorities and tentatively question the treatment of African Americans and Native Americans. Cary stands as a prime example of an author who has been overlooked and whose obscurity has hindered the construction of literary history, particularly in regard to the antebellum roots of realism and the influence of liberal religious belief on realistic fiction.

Galliher, Jane M.

2009-08-01T23:59:59.000Z

335

Nutrition and Biochemistry of PhospholipidsChapter 16 Effect of Two Diets in Children and Adolescents with Familial Hypercholesterolemia: Soy-Protein Diet Versus Low Saturated Fat Diet  

Science Conference Proceedings (OSTI)

Nutrition and Biochemistry of Phospholipids Chapter 16 Effect of Two Diets in Children and Adolescents with Familial Hypercholesterolemia: Soy-Protein Diet Versus Low Saturated Fat Diet Health Nutrition Biochemistry eChapters Health - Nu

336

Design and fabrication of 6.1-.ANG. family semiconductor devices using semi-insulating A1Sb substrate  

DOE Patents (OSTI)

For the first time, an aluminum antimonide (AlSb) single crystal substrate is utilized to lattice-match to overlying semiconductor layers. The AlSb substrate establishes a new design and fabrication approach to construct high-speed, low-power electronic devices while establishing inter-device isolation. Such lattice matching between the substrate and overlying semiconductor layers minimizes the formation of defects, such as threaded dislocations, which can decrease the production yield and operational life-time of 6.1-.ANG. family heterostructure devices.

Sherohman, John W. (Livermore, CA); Coombs, III, Arthur W. (Patterson, CA); Yee, Jick Hong (Livermore, CA); Wu, Kuang Jen J. (Cupertino, CA)

2007-05-29T23:59:59.000Z

337

A Personal Experience Reducing Radiation Exposures: Protecting Family in Kiev during the First Two Weeks after Chernobyl  

SciTech Connect

The Chernobyl nuclear reactor accident occurred in 1986. The plume from the explosions and fires was highly radioactive and resulted in very high exposure levels in the surrounding regions. This paper describes how the people in Kiev, Ukraine, a city 90 miles (120 km) south of Chernobyl, and in particular one individual in that city, Professor Vitaly Eremenko, became aware of the threat before the official announcement and the steps he took to mitigate potential impacts to his immediate family. The combination of being informed and using available resources led to greatly reduced consequences for his family and, in particular, his newborn granddaughter. He notes how quickly word of some aspects of the hazard spread in the city and how other aspects appear to not have been understood. Although these events are being recalled as the 20th anniversary of the terrible event approaches, the lessons are still pertinent today. Threats of possible terrorist use of radiation dispersal devices makes knowledge of effective individual actions for self-protection from radiation exposures a topic of current interest.

Eremenko, Vitaly A.; Droppo, James G.

2006-08-01T23:59:59.000Z

338

1754-6834-4-23.pdf  

NLE Websites -- All DOE Office Websites (Extended Search)

Bioprospecting Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases Biotechnology for Biofuels 2011, 4:23 doi:10.1186/1754-6834-4-23 Luen-Luen Li (luen.luen.li@univ-poitiers.fr) Safiyh Taghavi (taghavis@bnl.gov) Sean M McCorkle (mccorkle@bnl.gov) Yian-Biao Zhang (ybzhang@bnl.gov) Michael G Blewitt (blewitt@bnl.gov) Roman Brunecky (Roman.Brunecky@nrel.gov) William S Adney (Bill_Adney@nrel.gov) Michael E Himmel (Mike.Himmel@nrel.gov) Phillip Brumm (pbrumm@lucigen.com) Colleen Drinkwater (cdrinkwater@lucigen.com) David A Mead (dmead@lucigen.com) Susannah G Tringe (sgtringe@lbl.gov) Daniel van der Lelie (vdlelied@rti.org) ISSN 1754-6834 Article type Research Submission date 29 April 2011 Acceptance date 4 August 2011 Publication date 4 August 2011 Article URL http://www.biotechnologyforbiofuels.com/content/4/1/23 This

339

201117686 6537..6542  

NLE Websites -- All DOE Office Websites (Extended Search)

Introducing Introducing endo-xylanase activity into an exo-acting arabinofuranosidase that targets side chains Lauren S. McKee a,b , Maria J. Peña b , Artur Rogowski a , Adam Jackson a , Richard J. Lewis a , William S. York b , Kristian B. R. M. Krogh c , Anders Viksø-Nielsen c , Michael Skjøt c , Harry J. Gilbert a,b,1 , and Jon Marles-Wright a a Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne NE2 4HH, United Kingdom; b The Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602; and c Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark Edited by Arnold L. Demain, Drew University, Madison, NJ, and approved February 9, 2012 (received for review October 28, 2011) The degradation of the plant cell wall by glycoside hydrolases is central to environmentally sustainable industries. The major

340

November 19, 2011 1  

NLE Websites -- All DOE Office Websites (Extended Search)

9, 2011 1 9, 2011 1 2 S-layer homology (SLH) domain proteins Csac_0678 and Csac_2722 implicated in 3 plant polysaccharide deconstruction by the extremely thermophilic bacterium 4 Caldicellulosiruptor saccharolyticus 5 6 Inci Ozdemir, Sara E. Blumer-Schuette, and Robert M. Kelly* 7 8 Department of Chemical and Biomolecular Engineering, 9 North Carolina State University, Raleigh, NC 27695-7905 10 11 12 Submitted to: Applied and Environmental Microbiology (November, 2011) 13 AEM07031-11 (Revised Version of AEM05791-1) 14 15 Running title: S-layer homology domain proteins in C. saccharolyticus 16 17 Keywords: Caldicellulosiruptor saccharolyticus, extreme thermophile, plant biomass, 18 glycoside hydrolases, S-layer homology domains 19 20 21 *Address correspondence to: Robert M. Kelly 22

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

jbc.M110.215962.full.pdf  

NLE Websites -- All DOE Office Websites (Extended Search)

arabinan-specific arabinan-specific D D-1,2- arabinofuranosidase identified from screening the activities of bacterial GH43 glycoside hydrolases Alan Cartmell 1,2 *, Lauren McKee 1,2 *, Maria J. Peña 2 , Johan Larsbrink 3 , Harry Brumer 3 , Satoshi Kaneko 4 , Hitomi Ichinose 4 , Richard J. Lewis 1 , Anders Viksø-Nielsen 5 , Harry J. Gilbert 1,2¶ and Jon Marles-Wright 1 1 Institute for Cell and Molecular Biosciences, Newcastle University, The Medical School, Newcastle upon Tyne NE2 4HH, U.K; 2 The Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA; 3 School of Biotechnology, Royal Institute of Technology, AlbaNova University Centre, 10691 Stockholm, Sweden; 4 Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan,

342

Harnessing Glycosylation to Improve Cellulase Activity  

DOE Green Energy (OSTI)

Cellulases and hemicellulases are responsible for the turnover of plant cell wall polysaccharides in the biosphere, and thus form the foundation of enzyme engineering efforts in biofuels research. Many of these carbohydrate-active enzymes from filamentous fungi contain both N-linked and O-linked glycosylation, the extent and heterogeneity of which depends on growth conditions, expression host, and the presence of glycan trimming enzymes in the secretome, all of which in turn impact enzyme activity. As the roles of glycosylation in enzyme function have not been fully elucidated, here we discuss the potential roles of glycosylation on glycoside hydrolase enzyme structure and function after secretion. We posit that glycosylation, instead of hindering cellulase engineering, can be used as an additional tool to enhance enzyme activity, given deeper understanding of its molecular-level role in biomass deconstruction.

Beckham, G. T.; Dai, Z.; Matthews, J. F.; Momany, M.; Payne, C. M.; Adney, W. S.; Baker, S. E.; Himmel, M. E.

2012-06-01T23:59:59.000Z

343

Assessment of National Benefits from Retrofitting Existing Single-Family Homes with Ground Source Heat Pump Systems  

Science Conference Proceedings (OSTI)

This report assesses the potential national benefits of retrofitting U.S. single-family homes with state-of-the-art GSHP systems at various penetration rates. The benefits considered include energy savings, reduced summer electrical peak demand, consumer utility bill savings, and reduced carbon dioxide (CO2) emissions. The assessment relies heavily on energy consumption and other data obtained from the Residential Energy Consumption Survey conducted by the U.S. Department of Energy s Energy Information Administration. It also considers relative differences in energy consumption between a state-of-the-art GSHP system and existing residential space-heating, space-cooling, and water-heating (SH SC WH) systems, which were determined with a well-established energy analysis program for residential SH SC WH systems. The impacts of various climate and geological conditions, as well as the efficiency and market share of existing residential SH SC WH systems, have been taken into account in the assessment.

Liu, Xiaobing [ORNL

2011-01-01T23:59:59.000Z

344

Statewide Electricity and Demand Capacity Savings from the Implementation of IECC Code in Texas: Analysis for Single-Family Residences  

E-Print Network (OSTI)

This paper presents estimates of the statewide electricity and electric demand savings achieved from the adoption of the International Energy Conservation Code (IECC) for single-family residences in Texas and includes the corresponding increase in construction costs over the eight-year period from 2002 through 2009. Using the Energy Systems Laboratory's International Code Compliance Calculator (IC3) simulation tool, the annual statewide electricity savings in 2009 are estimated to be $161 million. The statewide peak electric demand reductions in 2009 are estimated to be 694 MW for the summer and 766 MW for the winter periods. Since 2002, the cumulative statewide electricity and electric demand savings over the eight year period from 2002 to 2009 are $1,803 million ($776 million from electricity savings and $1,027 million from electric demand savings) while the total increased costs are estimated to be $670 million.

Kim, H.; Baltazar, J.C.; Haberl, J.

2011-01-01T23:59:59.000Z

345

Solar Energy System Performance Evaluation: May-August 1978. Florida Gas Company, Single Family Residence, Winter Springs, Florida  

DOE Green Energy (OSTI)

A brief description of the system, which provides thermal energy for space heating, space cooling and domestic hot water heating for a one story single family dwelling, is given. A performance evaluation of the cooling subsystem is presented for the period May through August, 1978. A comparison of measured climatic data with long term average conditions for the vicinity is made. Subsystem performance, including collector array, storage, and space cooling systems, is discussed and design modifications that would improve the system's overall economic performance are considered. Space cooling is provided by an Arkla Model WF-35, 3-ton absorption cycle chiller. A comparison of the present system configuration with a vapor compression air conditioner is presented showing that net savings are realized when the present system is operated solely with solar supplied energy.

Lee, T.D.; McCumber, W.H.; Murphy, L.J.

1978-01-01T23:59:59.000Z

346

Preliminary market potential indexing study of the United States for direct gain in new single-family residential construction  

SciTech Connect

The evaluation of the market potential for passive solar designs in residential new construction offers an attractive counterpart to the numerous market penetration assessments that have been performed over the last four years. Market penetration analyses have generally concerned themselves with the long run adoption of solar energy technologies, while Market Potential Indexing (MPI) addressed here examines the near-term attractiveness of solar. The MPI method is briefly reviewed, followed by specification of six attributes that may characterize the residential single-family new construction market. Raw attribute data for each of the six is presented for 220 regions within the United States. Attribute weighting functions are constructed from the perspective of consumers, producers or home builders, and the federal government. Preliminary results from these three perspectives are portrayed for a fixed sized direct gain design.

Robson, W.M.; Roach, F.

1981-01-01T23:59:59.000Z

347

Blowup/scattering alternative for a discrete family of static critical solutions with various number of unstable eigenmodes  

E-Print Network (OSTI)

Decay of regular static spherically symmetric solutions in the SU(2) Yang-Mills-dilaton (YMd) system of equations under the independent excitation of their unstable eigenmodes has been studied self-consistently in the nonlinear regime. The considered regular YMd solutions form a discrete family and can be parametrised by the number $N=1,2,3,4...$ of their unstable eigenmodes in linear approximation. We have obtained strong numerical evidences in favour of the following statements: i) all static YMd solutions are distinct local threshold configurations, separating blowup and scattering solutions; ii) the main unstable eigenmodes are only those responsible for the blowup/scattering alternative; iii) excitation of higher unstable eigenmodes always leads to finite-time blowup; iv) the decay of the lowest N=1 static YMd solution via excitation of its unique unstable mode is an exceptional case because the resulting waves propagate as a whole without energy dispersion revealing features peculiar to solitons. Applic...

Donets, Evgeny E; Streltsova, Oksana I

2007-01-01T23:59:59.000Z

348

Standard Work Specifications for Single-Family Home Energy Upgrades Summary (Fact Sheet), Guidelines For Home Energy Professionals, Energy Efficiency & Renewable Energy (EERE)  

NLE Websites -- All DOE Office Websites (Extended Search)

Work Specifications Work Specifications for Single-Family Home Energy Upgrades Summary The U.S. Department of Energy (DOE), the National Renewable Energy Laboratory (NREL) and numer- ous industry stakeholders developed the Standard Work Specifications for Single-Family Home Energy Upgrades to define the minimum requirements for high- quality residential energy upgrades. The Standard Work Specifications for Single-Family Home Energy Upgrades is the first of three documents that will be published in 2012 and 2013 as part of the Guidelines for Home Energy Professionals project. Specifications for manufactured housing and multifamily homes will also be available. DOE, NREL, and industry developed the Standard Work Specifications under the Weatherization Assistance Program, building on more than 30 years of experience

349

Design, development and testing of a solar-powered multi-family residential-size prototype turbocompressor heat pump  

DOE Green Energy (OSTI)

An experimental program was conducted to further define, improve and demonstrate the performance characteristics and operational features of an existing 18-ton solar-powered prototype heat pump. The prototype heat pump is nominally sized for multi-family residential applications and provides both space heating and cooling. It incorporates a turbocompressor specially designed to operate at peak temperatures consistent with medium concentration collectors. The major efforts in this program phase included modification and improvement of the instrumentation sensors, the laboratory simulation equipment and selected heat pump components. After implementing these modifications, performance testing was conducted for a total operating time of approximately 250 hours. Experimental test results compared favorably with performance data calculated using the UTRC computer prediction program for the same boundary conditions. A series of tests was conducted continuously over a 12-h period to simulate operation (in the cooling mode) of the prototype heat pump under conditions typical of an actual installation. The test demonstrated that the heat pump could match the cooling load profile of a multi-family residential building. During the system performance testing, sufficient data were taken to identify the performance of each of the major components (e.g. turbine, compressor, heat exchangers, R11 pump). Component performance is compared with that calculated using the UTRC computer predict program and with data supplied by their manufacturers. The performance capabilities of the prototype heat pump system have been documented and recommendations are made for further design improvements which could be included in a MOD-2 configuration. The MOD-2 configuration would incorporate features that would improve system performance, reduce capital cost and most importantly improve system reliability.

Not Available

1982-10-01T23:59:59.000Z

350

Medical Certification -Family and Medical Leave Return to: Office of Human Resources FMLA Page 1 of 2 122 Kerr Admin Bldg, Corvallis, OR 97331-2132  

E-Print Network (OSTI)

Medical Certification - Family and Medical Leave Return to: Office of Human Resources ­ FMLA Page 1 Section II. Health Care Provider Completes this Section The above employee has requested medical leave to this request for medical information. Health Condition 1. Please indicate all categories applicable

Escher, Christine

351

SRTC criticality safety technical review: Phase 1 criticality analysis for the 9972-9975 family of shipping casks: (SRT-CMA-940003)  

SciTech Connect

Review of SRT-CMA-940003, ``Phase I Criticality Analysis For The 9972-9975 Family Of Shipping Casks (U). (SRT-CMA-940003).`` January 22, 1994, has been performed by the SRTC Applied Physics Group. The NCSE is a criticality assessment of the 9972-9975 family of shipping casks. This work is a follow-on of a previous criticality safety evaluation, with the differences between this and the previous evaluation are that now wall tolerances are modeled and more sophisticated analytical methods are applied. The NCSE under review concludes that, with one exception, the previously specified plutonium and uranium mass limits for 9972-9975 family of shipping casks do ensure that WSRC Nuclear Criticality Safety Manual requirements (ref. 1) are satisfied. The one exception is that the plutonium mass limit for the 9974 cask had to be reduced from 4.4 to 4.3 kg. In contrast, the 7.5 kg uranium mass limit for the 9974 cask was raised to 14.5 kg, making the uranium mass identical for all casks in this family. This technical review consisted of an independent check of the methods and models employed, application of ANSI/ANS 8.1 and 8.15, and verification of WSRC Nuclear Criticality Safety Manual procedures.

Rathbun, R.

1994-03-02T23:59:59.000Z

352

Neutrino oscillations in an SO(10) supersymmetric grand unified theory with U(2)xU(1){sup n} family symmetry  

Science Conference Proceedings (OSTI)

In a previous paper we analyzed fermion masses (focusing on neutrino masses and mixing angles) in an SO(10) SUSY GUT with U(2)xU(1){sup n} family symmetry. The model is ''natural'' containing all operators in the Lagrangian consistent with the states and their charges. With minimal family symmetry breaking vacuum expectation values (VEVs) the model is also predictive giving a unique solution to atmospheric (with maximal {nu}{sub {mu}}{yields}{nu}{sub {tau}} mixing) and solar (with SMA MSW {nu}{sub e}{yields}{nu}{sub s} mixing) neutrino oscillations. In this paper we analyze the case of general family breaking VEVs. We now find several new solutions for three, four, and five neutrinos. For three neutrinos we now obtain SMA MSW, LMA MSW, or vacuum oscillation solutions for solar neutrinos. In all three cases the atmospheric data are described by maximal {nu}{sub {mu}}{yields}{nu}{sub {tau}} mixing. In the four and five neutrino cases, in addition to fitting atmospheric and solar data as before, we are now able to fit LSND data. All this is obtained with the additional parameters coming from the family symmetry breaking VEVs, providing only minor changes in the charged fermion fits. (c) 2000 The American Physical Society.

Blazek, T. [Department of Physics and Astronomy, Northwestern University, Evanston, Illinois 60208 (United States); Raby, S. [Department of Physics, The Ohio State University, 174 W. 18th Ave., Columbus, Ohio 43210 (United States); Tobe, K. [Department of Physics, The Ohio State University, 174 W. 18th Ave., Columbus, Ohio 43210 (United States)

2000-09-01T23:59:59.000Z

353

A generalized family of fixed-radius distribution-based distance measures for content-based fMRI image retrieval  

Science Conference Proceedings (OSTI)

We present a family of distance measures for comparing activation patterns captured in fMRI images. We model an fMRI image as a spatial object with varying density, and measure the distance between two fMRI images using a novel fixed-radius, distribution-based ... Keywords: Brain imaging, Content-based image retrieval, fMRI image matching

John Novatnack; Nicu Cornea; Ali Shokoufandeh; Deborah Silver; Sven Dickinson; Paul Kantor; Bing Bai

2008-09-01T23:59:59.000Z

354

Estimates of Energy Cost Savings Achieved from 2009 IECC Code-Compliant, Single Family Residences in Texas  

E-Print Network (OSTI)

This report presents estimates of the energy cost savings to be achieved from 2009 International Energy Conservation Code (IECC) code-compliant, single-family residences in Texas compared to the pre-2009 IECC codes, including: the 2001 IECC, the 2006 IECC, and the 2006 IECC w/ Houston amendments (w/ HA). A series of simulations were performed using an ESL simulation model (BDL version 4.01.07 of IC3) based on the DOE-2.1e simulation and the appropriate TMY2 weather files for three counties representing three 2009 IECC Climate Zones across Texas: Harris County for Climate Zone 2, Tarrant County for Climate Zone 3, and Potter County for Climate Zone 4. Two options based on the choice of heating fuel type were considered: (a) an electric/gas house (gas-fired furnace for space heating, and gas water heater for domestic water heating), and (b) a heat pump house (heat pump for space heating, and electric water heater for domestic water heating). The base-case building was assumed to be a 2,325 sq. ft., square-shape, one story, single-family, detached house with a floor-to-ceiling height of 8 feet. The house has an attic with a roof pitched at 23 degrees. The base-case building envelope and system characteristics were determined from the general characteristics and the climate-specific characteristics as specified in the 2001 IECC, the 2006 IECC, the 2006 IECC w/HA, and the 2009 IECC. In addition, to facilitate a better comparison with the 2009 code, several modifications were applied to the pre-2009 IECC codes. As a result, the estimated annual energy cost savings per house associated with the 2009 IECC compared to the 2001 and 2006 IECC are: (a) an electric/gas house: $462/year and $206/year for Harris County, $432/year and $216/year for Tarrant County, and $576/year and $153/year for Potter County and (b) a heat pump house: $490/year and $203/year for Harris County, $487/year and $226/year for Tarrant County, and $680/year and $155/year for Potter County. The corresponding % savings of total energy cost of a 2009 IECC code-compliant house are: (a) an electric/gas house: 22.7% and 10.1% for Harris County, 21.8% and 10.9% for Tarrant County, and 28.9% and 7.7% for Potter County and (b) a heat pump house: 21.6% and 8.9% for Harris County, 20.9% and 9.7% for Tarrant County, and 25.7% and 5.8% for Potter County.

Kim, H.; Baltazar, J. C.; Haberl, J.

2011-01-01T23:59:59.000Z

355

T2SOLV: An enhanced package of solvers for the TOUGH2 family of reservoir simulation codes  

DOE Green Energy (OSTI)

T2SOLV is an enhanced package of matrix solvers for the TOUGH2 family of codes. T2SOLV includes all the Preconditioned Conjugate Gradient (PCG) solvers used in T2CG1, the current solver package, as well as LUBAND, a new direct solver, and DLUSTB, a PCG solver based on the BiCGSTAB method. Additionally, T2SOLV includes the D4 grid numbering scheme and two sets of preprocessors. Results from test problems indicate that LUBAND is faster, more reliable and requires less storage than MA28, the current direct solver. BiCGSTAB solver is shown to be superior to the other PCG methods in T2SOLV. Finally, the preprocessors improve the performance of the PCG solvers and allow the solution of previously intractable problems. TOUGH2 is capable of modeling most of the processes arising in the natural state of geothermal reservoirs and in response to production and injection operations. It can handle the appearance and disappearance of liquid and vapor phases, boiling and condensation, multiphase flow due to pressure, gravity, and capillary forces, vapor adsorption with vapor pressure lowering, heat conduction, and heat exchange between rocks and fluids. It is applicable to flow systems of arbitrary geometry from one to three dimensions, and has special provisions for flow in fractured-porous media.

Moridis, G.; Pruess, K. [Lawrence Berkeley National Lab., CA (United States). Earth Sciences Div.

1997-11-01T23:59:59.000Z

356

Blowup/scattering alternative for a discrete family of static critical solutions with various number of unstable eigenmodes  

E-Print Network (OSTI)

Decay of regular static spherically symmetric solutions in the SU(2) Yang-Mills-dilaton (YMd) system of equations under the independent excitation of their unstable eigenmodes has been studied self-consistently in the nonlinear regime. The considered regular YMd solutions form a discrete family and can be parametrised by the number $N=1,2,3,4...$ of their unstable eigenmodes in linear approximation. We have obtained strong numerical evidences in favour of the following statements: i) all static YMd solutions are distinct local threshold configurations, separating blowup and scattering solutions; ii) the main unstable eigenmodes are only those responsible for the blowup/scattering alternative; iii) excitation of higher unstable eigenmodes always leads to finite-time blowup; iv) the decay of the lowest N=1 static YMd solution via excitation of its unique unstable mode is an exceptional case because the resulting waves propagate as a whole without energy dispersion revealing features peculiar to solitons. Applications of the obtained results to Type-I gravitational collapse of massless fields are briefly discussed.

Evgeny E. Donets; Edik A. Hayryan; Oksana I. Streltsova

2007-11-26T23:59:59.000Z

357

Lithium Polysulfidophosphates: A Family of Lithium-Conducting Sulfur-Rich Compounds for Lithium-Sulfur Batteries  

SciTech Connect

Given the great potential for improving the energy density of state-of-the-art lithium-ion batteries by a factor of 5, a breakthrough in lithium-sulfur (Li-S) batteries will have a dramatic impact in a broad scope of energy related fields. Conventional Li-S batteries that use liquid electrolytes are intrinsically short-lived with low energy efficiency. The challenges stem from the poor electronic and ionic conductivities of elemental sulfur and its discharge products. We report herein lithium polysulfidophosphates (LPSP), a family of sulfur-rich compounds, as the enabler of long-lasting and energy-efficient Li-S batteries. LPSP have ionic conductivities of 3.0 10-5 S cm-1 at 25 oC, which is 8 orders of magnitude higher than that of Li2S (~10-13 S cm-1). The high Li-ion conductivity of LPSP is the salient characteristic of these compounds that impart the excellent cycling performance to Li-S batteries. In addition, the batteries are configured in an all-solid state that promises the safe cycling of high-energy batteries with metallic lithium anodes.

Lin, Zhan [ORNL; Liu, Zengcai [ORNL; Fu, Wujun [ORNL; Dudney, Nancy J [ORNL; Liang, Chengdu [ORNL

2013-01-01T23:59:59.000Z

358

Using a Family of Dividing Surfaces Normal to the Minimum EnergyPath for Quantum Instanton Rate Constants  

SciTech Connect

One of the outstanding issues in the quantum instanton (QI) theory (or any transition state-type theory) for thermal rate constants of chemical reactions is the choice of an appropriate ''dividing surface'' (DS) that separates reactants and products. (In the general version of the QI theory, there are actually two dividing surfaces involved.) This paper shows one simple and general way for choosing DS's for use in QI Theory, namely using the family of (hyper) planes normal to the minimum energy path (MEP) on the potential energy surface at various distances s along it. Here the reaction coordinate is not one of the dynamical coordinates of the system (which will in general be the Cartesian coordinates of the atoms), but rather simply a parameter which specifies the DS. It is also shown how this idea can be implemented for an N-atom system in 3d space in a way that preserves overall translational and rotational invariance. Numerical application to a simple system (the colliner H + H{sub 2} reaction) is presented to illustrate the procedure.

Li, Yimin; Miller, Wlliam H.

2006-02-22T23:59:59.000Z

359

SIMULATED BUILDING ENERGY PERFORMANCE OF SINGLE-FAMILY DETACHED RESIDENCES DESIGNED FOR OFF-GRID, OFF-PIPE OPERATION  

SciTech Connect

This paper presents the analysis of energy performance of single-family detached homes in three U.S. climates, in order to determine energy-efficiency measures for minimizing the loads and sizing requirements of renewable energy systems that are essential for its offgrid, off-pipe (i.e., utility-independent) operation. The analysis used a DOE-2.1e simulation model of a 2000/2001 IECC (International Energy Conservation Code) standard house as a base case in three climate locations: Minneapolis, MN, Atlanta, GA, and Phoenix, AZ. This selection of measures and determination of loads for renewable energy systems were accomplished by analyzing the energy use using DOE-2.1e simulations and heating/cooling load components using the Manual J Average Load Procedure. The analysis showed several aspects of building energy performance during different times of the year in terms of available energy resources that are critical for the sizing, utilization, and cost effectiveness of renewable energy systems.

Malhotra, Mini [ORNL; Haberl, Dr. Jeff S. [Texas A& M University

2010-01-01T23:59:59.000Z

360

TRC Bibliographies: Family Activities  

NLE Websites -- All DOE Office Websites (Extended Search)

science with your children. Doing science activities together develops both parenting skills and the child's learning, problem solving and scientific skills. Participating in...

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Product Family Glossary  

E-Print Network (OSTI)

Printed on Recycled PaperIMPORTANT NOTICE Texas Instruments (TI) reserves the right to make changes to its products or to discontinue any semiconductor product or service without notice, and advises its customers to obtain the latest version of relevant information to verify, before placing orders, that the information being relied on is current. TI warrants performance of its semiconductor products and related software to the specifications applicable at the time of sale in accordance with TIs standard warranty. Testing and other quality control techniques are utilized to the extent TI deems necessary to support this warranty. Specific testing of all parameters of each device is not necessarily performed, except those mandated by government requirements. Certain applications using semiconductor products may involve potential risks of death, personal injury, or severe property or environmental damage (Critical Applications).

Sprua Glossary; Tms Dsp; Literature Number Sprua

1998-01-01T23:59:59.000Z

362

Fermilab Family Open House  

NLE Websites -- All DOE Office Websites (Extended Search)

Sunday, February 9, 2014 Sunday, February 9, 2014 1:00–5:00 PM Directions to Fermilab This is a party for children who bring an adult with them to learn about the world of physics. (There's plenty for the grown-ups, too.) Events include: Watch Mr. Freeze's fabulous cryogenics show Explore physics concepts with hands-on activities Ask a scientist your physics questions. Take a tour! And more! The Open House is most appropriate for children in grades 3 and up. The event is free. Register only if you wish to go on a tour (minimum age 10). Otherwise, you do not have to register. You should pick up the tickets for the tours in the atrium on the day of the event. Tickets not picked up at least 10 minutes before a tour starts will be released. The Open House is co-supported by Fermilab Friends for Science Education and the Education Office.

363

The Smartweed Family  

NLE Websites -- All DOE Office Websites (Extended Search)

breakfast" at his house -- stacks of buckwheat pancakes made with freshly ground flour just received from his kin in Pennsylvania, covered with gobs of butter and...

364

1996-2004 Trends in the Single-Family Housing Market: Spatial Analysis of the Residential Sector  

SciTech Connect

This report provides a detailed geographic analysis of two specific topics affecting the residential sector. First, we performed an analysis of new construction market trends using annual building permit data. We report summarized tables and national maps to help illustrate market conditions. Second, we performed a detailed geographic analysis of the housing finance market. We analyzed mortgage application data to provide citable statistics and detailed geographic summarization of the residential housing picture in the US for each year in the 1996-2004 period. The databases were linked to geographic information system tools to provide various map series detailing the results geographically. Looking at these results geographically may suggest potential new markets for TD programs addressing the residential sector that have not been considered previously. For example, we show which lenders affect which regions and which income or mortgage product classes. These results also highlight the issue of housing affordability. Energy efficiency R&D programs focused on developing new technology for the residential sector must be conscious of the costs of products resulting from research that will eventually impact the home owner or new home buyer. Results indicate that home values as a proportion of median family income in Building America communities are closely aligned with the national average of home value as a proportion of median income. Other key findings: The share of home building and home buying activity continues to rise steadily in the Hot-Dry and Hot-Humid climate zones, while the Mixed-Humid and Cold climate zone shares continue to decline. Other zones remain relatively stable in terms of share of housing activity. The proportion of home buyers having three times the median family income for their geography has been steadily increasing during the study period. Growth in the Hispanic/Latino population and to a lesser degree in the Asian population has translated into proportional increases in share of home purchasing by both groups. White home buyers continue to decline as a proportion all home buyers. Low interest rate climate resulted in lenders moving back to conventional financing, as opposed to government-backed financing, for cases that would be harder to financing in higher rate environments. Government loan products are one mechanism for affecting energy efficiency gains in the residential sector. The rate environment and concurrent deregulation of the finance industry resulted unprecedented merger and acquisition activity among financial institutions during the study period. This study conducted a thorough accounting of this merger activity to inform the market share analysis provided. The home finance industry quartiles feature 5 lenders making up the first quartile of home purchase loans, 18 lenders making up the second quartile, 111 lenders making up the third quartile, and the remaining nearly 8,000 lenders make up the fourth quartile.

Anderson, Dave M.; Elliott, Douglas B.

2006-09-05T23:59:59.000Z

365

Mapping of a possible X-linked form of familial developmental dysphasia (FDD) in a single large pedigree  

Science Conference Proceedings (OSTI)

Children diagnosed with developmental dysphasia develop speech very late without exhibiting sensory or motor dysfunction, and when they do begin to speak their grammar is abnormal. A large three-generation British pedigree was recently identified in which 16 out of 30 members were diagnosed as dysphasic. Assuming a dominant mode of inheritance with homogeneous phenotypic expression and complete penetrance among affected members, we showed by simulation analysis that this pedigree has the power to detect linkage to marker loci with an average maximum LOD score of 3.67 at {theta}=0.1. Given the absence of male-to-male transmission and a ratio of female to male affecteds (10/6) in this pedigree within the expected range for an X-linked dominant mode of inheritance, we decided to begin a genome-wide linkage analysis with microsatellite markers on the human X chromosome. Fifteen individuals (10 affected) from three generations were genotyped with 35 polymorphic STS`s (Research Genetics) which were approximately uniformly distributed along the X chromosome. Two-point linkage was assessed using the MLINK and ILINK programs from the LINKAGE package. Markers DXS1223, DXS987, DXS996 and DXS1060 on Xp22 showed consistent linkage to the disease locus with a maximum LOD score of 0.86 at a distance of 22 cM for DXS1060. If further analysis with additional markers and additional family members confirms X-linkage, such a localization would provide support for Lehrke`s hypothesis for X-linkage of major intellectual traits including verbal functioning.

Dunne, P.W.; Doody, R.S.; Epstein, H.F. [Baylor College of Medicine, Houston, TX (United States)] [and others

1994-09-01T23:59:59.000Z

366

Conserved synteny at the protein family level reveals genes underlying Shewanella species cold tolerance and predicts their novel phenotypes  

Science Conference Proceedings (OSTI)

Bacteria of the genus Shewanella can thrive in different environments and demonstrate significant variability in their metabolic and ecophysiological capabilities including cold and salt tolerance. Genomic characteristics underlying this variability across species are largely unknown. In this study we address the problem by a comparison of the physiological, metabolic and genomic characteristics of 19 sequenced Shewanella species. We have employed two novel approaches based on association of a phenotypic trait with the number of the trait-specific protein families (Pfam domains) and on the conservation of synteny (order in the genome) of the trait-related genes. Our first approach is top-down and involves experimental evaluation and quantification of the species cold tolerance followed by identification of the correlated Pfam domains and genes with a conserved synteny. The second, a bottom-up approach, predicts novel phenotypes of the species by calculating profiles of each Pfam domain among their genomes and following pair-wise correlation of the profiles and their network clustering. Using the first approach we find a link between cold and salt tolerance of the species and the presence in the genome of a Na+/H+ antiporter gene cluster. Other cold tolerance related genes includes peptidases, chemotaxis sensory transducer proteins, a cysteine exporter, and helicases. Using the bottom-up approach we found several novel phenotypes in the newly sequenced Shewanella species, including degradation of aromatic compounds by an aerobic hybrid pathway in S. woodyi, degradation of ethanolamine by S. benthica, and propanediol degradation by S. putrefaciens CN32 and S. sp. W3-18-1.

Karpinets, Tatiana V.; Obraztsova, Anna; Wang, Yanbing; Schmoyer, Denise D.; Kora, Guruprasad; Park, Byung H.; Serres, Margrethe H.; Romine, Margaret F.; Land, Miriam L.; Kothe, Terence B.; Fredrickson, Jim K.; Nealson, Kenneth H.; Uberbacher, Edward

2010-03-01T23:59:59.000Z

367

WORKINGPAPER SERIES Number 150CAP AND DIVIDEND: HOW TO CURB GLOBAL WARMING WHILE PROTECTING THE INCOMES OF AMERICAN FAMILIES  

E-Print Network (OSTI)

This essay examines the distributional effects of a cap-and-dividend policy for reducing carbon emission in the United States: a policy that auctions carbon permits and rebates the revenue to the public on an equal per capita basis. The aim of the policy is to reduce U.S. emissions of carbon dioxide, the main pollutant causing global warming, while at the same time protecting the real incomes of middle-income and lower-income American families. The number of permits is set by a statutory cap on carbon emissions that gradually diminishes over time. The sale of carbon permits will generate very large revenues, posing the critical question of who will get the money. The introduction of carbon permits or, for that matter, any policy to curb emissions will raise prices of fossil fuels, Key words: Global warming; fossil fuels; climate change; carbon permits; cap-and-dividend; cap-and-auction; cap-and-trade. and have a regressive impact on income distribution, since fuel expenditures represent a larger fraction of income for lower-income households than for upper-income households. The net effect of carbon emission-reduction policies depends on who gets the money that households pay in higher prices. We find that a cap-and-dividend policy would have a strongly progressive net effect. Moreover, the majority of U.S. households would be net winners in purely monetary terms: that is, their real incomes, after paying higher fuel prices and receiving their dividends, would rise. From the standpoints of both distributional equity and political feasibility, a cap-and-dividend policy is therefore an attractive way to curb carbon emissions. s s

James K. Boyce; Matthew Riddle; James K. Boyce; Matthew Riddle

2007-01-01T23:59:59.000Z

368

eat-5 and unc-7 represent a multigene family in Caenorhabditis elegans involved in cell-cell coupling. J. Cell Biol. 134, 537548. Abstract Article  

E-Print Network (OSTI)

Abstract. The Drosophila melanogaster genes Passover and l(1)ogre and the Caenorhabditis elegans gene unc-7 define a gene family whose function is not known. We have isolated and characterized the C. elegans gene eat-5, which is required for synchronized pharyngeal muscle contractions, and find that it is a new member of this family. Simultaneous electrical and video recordings reveal that in eat-5 mutants, action potentials of muscles in the anterior and posterior pharynx are unsynchronized. Injection of carboxyfluorescein into muscles of the posterior pharynx demonstrates that all pharyngeal muscles are dye-coupled in wild-type animals; in eat-5 mutants, however, muscles of the anterior pharynx are no longer dye-coupled to posterior pharyngeal muscles. We show that a gene fusion of eat-5 to the green fluo-

Todd A. Starich; Raymond Y. N. Lee; Cristin Panzareua; Leon Avery; Jocelyn E. Shaw

1996-01-01T23:59:59.000Z

369

A 1.5 A resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase  

NLE Websites -- All DOE Office Websites (Extended Search)

1498 1498 doi:10.1107/S1744309111038449 Acta Cryst. (2011). F67, 1498-1500 Acta Crystallographica Section F Structural Biology and Crystallization Communications ISSN 1744-3091 A 1.5 A ˚ resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase Markus Alahuhta, a Puja Chandrayan, b Irina Kataeva, b Michael W. W. Adams, b Michael E. Himmel a and Vladimir V. Lunin a * a BioSciences Center, National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, CO 80401, USA, and b Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602-7229, USA Correspondence e-mail: vladimir.lunin@nrel.gov Received 17 August 2011 Accepted 19 September 2011 PDB Reference: family 3 pectate lyase catalytic module, 3t9g. A 1.5 A ˚ resolution X-ray structure of the catalytic module of Caldicellulosi- ruptor bescii

370

Further localization of the gene for nevoid basal cell carcinoma syndrome (NBCCS) in 15 Australasian families: Linkage and loss of heterozygosity  

Science Conference Proceedings (OSTI)

Nevoid basal cell carcinoma syndrome (NBCCS; basal cell nevus syndrome or Gorlin syndrome) is a cancer-predisposition syndrome characterized by multiple basal cell carcinomas (BCCs) and diverse developmental defects. The gene for NBCCS has been mapped to 9q23.1-q31 in North Americal and European families. In addition, loss of heterozygosity (LOH) for genetic markers in this region has been detected in sporadic BCCs, indicating that the NBCCs gene is probably a tumor-suppressor gene. In this study the authors have determined that the NBCCS gene is also linked to this region in Australasian pedigrees and that there is no significant evidence of heterogeneity. They have defined the localization of the gene by multipoint and haplotype analysis of 15 families, using four microsatellite markers. LOH at these loci was detected in 50% of sporadic BCCs, a rate that is significantly higher than that in other skin lesions used as controls. 21 refs., 3 figs., 2 tabs.

Chenevix-Trench, G.; Wicking, C.; Berkman, J.; Sharpe, H.; Hockey, A.; Haan, E.; Oley, C.; Ravine, D.; Turner, A.; Searle, J. (and others)

1993-09-01T23:59:59.000Z

371

Assessing the Significance of Above- and Belowground Carbon Allocation of Fast- and Slow-Growing Families of Loblolly Pine - Final Report  

DOE Green Energy (OSTI)

During this project we experimentally evaluated the below-ground biomass and carbon allocation and partitioning of four different fast- and slow-growing families of loblolly pine located in Scotland County, NC, in an effort to increase the long-term performance of the crop. The trees were subjected to optimal nutrition and control since planting in 1993. Destructive harvests in 1998 and 2000 were used for whole?plant biomass estimates and to identify possible family differences in carbon acquisition (photosynthesis) and water use efficiency. At regular intervals throughout each year we sampled tissues for carbohydrate analyses to assess differences in whole-tree carbon storage. Mini rhizotron observation tubes were installed to monitor root system production and turnover. Stable isotope analysis was used to examine possible functional differences in water and nutrient acquisition of root systems between the various families. A genetic dissection of root ontogenic and architectural traits, including biomass partitioning, was conducted using molecular markers to better understand the functional implications of these traits on resource acquisition and whole-plant carbon allocation.

Topa, M. A.; Weinstein, D. A.; Retzlaff, W. A.

2001-03-01T23:59:59.000Z

372

Structural, Biochemical, and Phylogenetic Analyses Suggest That Indole-3-Acetic Acid Methyltransferase Is an Evolutionarily Ancient Member of the SABATH Family  

SciTech Connect

The plant SABATH protein family encompasses a group of related small-molecule methyltransferases (MTs) that catalyze the S-adenosyl-L-methionine-dependent methylation of natural chemicals encompassing widely divergent structures. Indole-3-acetic acid (IAA) methyltransferase (IAMT) is a member of the SABATH family that modulates IAA homeostasis in plant tissues through methylation of IAA's free carboxyl group. The crystal structure of Arabidopsis (Arabidopsis thaliana) IAMT (AtIAMT1) was determined and refined to 2.75 Angstroms resolution. The overall tertiary and quaternary structures closely resemble the two-domain bilobed monomer and the dimeric arrangement, respectively, previously observed for the related salicylic acid carboxyl methyltransferase from Clarkia breweri (CbSAMT). To further our understanding of the biological function and evolution of SABATHs, especially of IAMT, we analyzed the SABATH gene family in the rice (Oryza sativa) genome. Forty-one OsSABATH genes were identified. Expression analysis showed that more than one-half of the OsSABATH genes were transcribed in one or multiple organs. The OsSABATH gene most similar to AtIAMT1 is OsSABATH4. Escherichia coli-expressed OsSABATH4 protein displayed the highest level of catalytic activity toward IAA and was therefore named OsIAMT1. OsIAMT1 exhibited kinetic properties similar to AtIAMT1 and poplar IAMT (PtIAMT1). Structural modeling of OsIAMT1 and PtIAMT1 using the experimentally determined structure of AtIAMT1 reported here as a template revealed conserved structural features of IAMTs within the active-site cavity that are divergent from functionally distinct members of the SABATH family, such as CbSAMT. Phylogenetic analysis revealed that IAMTs from Arabidopsis, rice, and poplar (Populus spp.) form a monophyletic group. Thus, structural, biochemical, and phylogenetic evidence supports the hypothesis that IAMT is an evolutionarily ancient member of the SABATH family likely to play a critical role in IAA homeostasis across a wide range of plants.

Zhao,N.; Ferrer, J.; Ross, J.; Guan, J.; Yang, Y.; Pichersky, E.; Noel, J.; Chen, F.

2008-01-01T23:59:59.000Z

373

Both Mom and Dad Should Go to Work so the Family Would Have More Money: Childrens Attitudes Towards Occupational Gender Roles in the Village of Metztitln, Mexico  

E-Print Network (OSTI)

so the Family W oul d Have More Money: Childrens Villagetheir dissatisfaction to the lack of money or boredom. Thewho wanted to make her own money and be self- sufficient.

Milicevic, Zorana

2010-01-01T23:59:59.000Z

374

Systematic Analysis of Sequences and Expression Patterns of Drought-Responsive Members of the HD-Zip Gene Family in Maize  

E-Print Network (OSTI)

Background: Members of the homeodomain-leucine zipper (HD-Zip) gene family encode transcription factors that are unique to plants and have diverse functions in plant growth and development such as various stress responses, organ formation and vascular development. Although systematic characterization of this family has been carried out in Arabidopsis and rice, little is known about HD-Zip genes in maize (Zea mays L.). Methods and Findings: In this study, we described the identification and structural characterization of HD-Zip genes in the maize genome. A complete set of 55 HD-Zip genes (Zmhdz1-55) were identified in the maize genome using Blast search tools and categorized into four classes (HD-Zip I-IV) based on phylogeny. Chromosomal location of these genes revealed that they are distributed unevenly across all 10 chromosomes. Segmental duplication contributed largely to the expansion of the maize HD-ZIP gene family, while tandem duplication was only responsible for the amplification of the HD-Zip II genes. Furthermore, most of the maize HD-Zip I genes were found to contain an overabundance of stress-related ciselements in their promoter sequences. The expression levels of the 17 HD-Zip I genes under drought stress were also investigated by quantitative real-time PCR (qRT-PCR). All of the 17 maize HD-ZIP I genes were found to be regulated by drought stress, and the duplicated genes within a sister pair exhibited the similar expression patterns, suggesting their conserved functions during the process of evolution.

Yang Zhao; Yuqiong Zhou; Haiyang Jiang; Xiaoyu Li; Defang Gan; Xiaojian Peng; Suwen Zhu

2011-01-01T23:59:59.000Z

375

The House of 'Obs-mtsho - The History of a Bhutanese Gentry Family from the 13th to the 20th Century  

E-Print Network (OSTI)

, History of Bhutan. Land of the Peaceful Dragon. Thimphu, Education Dept. of Bhutan, 1980: 30. 1 Giuseppe Tucci, Tibetan Painted Scrolls, vol. 2: 662ff; Franco Ricca & Erberto Lo Bue, The Great Stupa of Gyantse. London: Serindia Publications, 1993: 14... Gyal-mtshan and others. He was nominated as the 24th rJe mKhan-po in 1831 and served for 5 years. He died in office at the age of 47 (1836). 2.3 Chos-rje Phrin-las rGyal-mtshan (1839-1898) He was born into the Amorimu family line. He studied with Tshul...

Ardussi, John A

2000-01-01T23:59:59.000Z

376

Family and home correlates of children's physical activity in a multi-ethnic population: the cross-sectional Child Heart and Health Study in England (CHASE)  

E-Print Network (OSTI)

J Griffin1, Derek G Cook2, Chris G Owen2, Alicja R Rudnicka2, Peter H Whincup2 Abstract Background: The influence of the family and home environment on childhood physical activity (PA) and whether this differs between ethnic groups remains uncertain... and implications. Edited by: Biddle S, Sallis J, Cavill N. London: Health Education Authority; 1998:49-68. 5. Parfitt G, Pavey T, Rowlands AV: Childrens physical activity and psychological health: the relevance of intensity. Acta Paediatr 2009, 98(6):1037-1043. 6...

McMinn, Alison M; van Sluijs, Esther M F; Nightingale, Claire M; Griffin, Simon J; Cook, Derek G; Owen, Chris G; Rudnicka, Alicja R; Whincup, Peter H

2011-02-15T23:59:59.000Z

377

Test for Modeling Windows in DOE 2.1E for Comparing the Window Library with the Shading Coefficient Method for a Single-Family Residence in Texas  

E-Print Network (OSTI)

This study examines the difference of the window simulation test between the Shading Coefficient (SC) and the Window Library (WL) Methods on DOE 2.1E of the 2000 IECC (International Energy Conservation Code) for single-family residences in Texas. The window simulation tests are performed using single-pane, double-pane, and low-e glass on two standard DOE 2.1E single-family house models: 1) the model which has the R-value for wall, roof and floor according to 2000 IECC (Quick Wall), and 2) the model which has the real wood frame wall and has the same R-value as the first one (Thermal Wall). The analysis showed different results according to the types of the glass, simulation method (Shading Coefficient or Window Library), and types of wall (quick wall and thermal wall). The saving of daily peak heating (kBtu/day) from single-pane to low-e glass on thermal mass and quick wall shows the most variation.

Kim, S.; Haberl, J. S.

2008-07-18T23:59:59.000Z

378

fulltext.pdf  

NLE Websites -- All DOE Office Websites (Extended Search)

Evolution Evolution and divergence in the coding and promoter regions of the Populus gene family encoding xyloglucan endotransglycosylase/hydrolases Xia Y e & Suhua Yuan & Hong Guo & Feng Chen & Gerald A. Tuskan & Zong-Ming Cheng Received: 8 December 2010 / Revised: 11 August 2011 / Accepted: 18 August 2011 # Springer-V erlag 2011 Abstract Xyloglucan endotransglycosylase/hydrolases (XTHs) are believed to modify the cell wall structure by cleaving a xyloglucan polymer and transferring the newly generated, potentially reducing, terminal to another xylo- glucan. We report here the detailed analysis of 37 Populus trichocarpa XTH genes/proteins in their divergence in both the coding and 5' promoter regions. Our results show that the Populus XTH genes have experienced whole-genome and local duplications and pre- and post-speciation diver- gence. Genome-wide and segmental

379

Modeling-Assisted Growth of New Multiferroics in the MTiO3 (M = Fe, Mn, Ni) Family as Epitaxial Thin Films  

NLE Websites -- All DOE Office Websites (Extended Search)

Modeling-Assisted Growth of New Multiferroics in the MTiO 3 (M = Fe, Mn, Ni) Family as Epitaxial Thin Films Project start date: July, 2011 EMSL Lead Investigator: Tamas Varga Spectroscopy and Diffraction Group, EMSL, PNNL Co-investigators: Mark E. Bowden (EMSL), Timothy C. Droubay (FCSD), Scott A. Chambers (FCSD), Bernd C. Kabius (EMSL), Edoardo Apra (EMSL), and William A. Shelton (EMSL) Collaborators: Trudy B. Bolin (Advanced Photon Source, Argonne National Laboratory, Argonne, IL), Peter E. Schiffer and Venkatraman Gopalan (Pennsylvania State University, University Park, PA) Materials that show simultaneous electric and magnetic order are currently gaining increasing attention due to the fact that such multiferroics show promise for the design of new multifunctional

380

A new family of anion receptors and their effects on ion pair dissociation and conductivity of lithium salts in non-aqueous solutions  

DOE Green Energy (OSTI)

A new family of anion receptors based on aza-ether compounds have been synthesized. Since the anion complexation of these compounds is not based on either positively charged sites or hydrogen bonding, they have a potential to be used in lithium batteries as electrolyte additives. When these compounds are added into nonaqueous electrolytes using lithium salts, such as LiCl/BF or LiBr/THF, the ionic conductivity can be dramatically increased. Near Edge X-ray Absorption Fine Structure (NF-XAFS) spectroscopy studies show that Cl{sup {minus}} anions are completed with the nitrogen groups in these compounds. The increase in ionic conductivity and the degree of complexation, are both related to the number of R=CF{sub 3}SO{sub 2} groups that are used to substitute the amine hydrogen atoms in these aza-ether compounds.

Lee, H.S.; Yang, X.Q.; McBreen, J. [Brookhaven National Lab., Upton, NY (United States); Okamoto, Y. [Polytechnic Univ., Brooklyn, NY (United States)

1994-08-01T23:59:59.000Z

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Detailed Analysis of the Thermal Mass Credits in a Code-Traceable DOE-2 Simulation of the 2001 IECC for a Single-Family Residence in Texas  

E-Print Network (OSTI)

This paper presents the results of a study that investigates the thermal mass credits in the 2001 International Energy Conservation Code (IECC) (ICC 1999, 2001) for a single-family residence in Texas using the DOE-2 building energy simulation program. In this analysis seven different wall types were simulated, and each wall type was matched to the recommended overall U-value of a lightweight wall that meets the prescriptive specifications of the 2001 IECC. This paper presents an analysis of the total annual cooling and heating energy use for wall types with varying thermal mass, and thermostat settings, as well as recommendations concerning the most energy-efficient wall type, and includes input specification methods using the DOE-2 program

Kim, S.; Haberl, J.

2008-12-01T23:59:59.000Z

382

Li6La3SnMO12 (M = Sb, Nb, Ta), a Family of Lithium Garnets with High Li-Ion Conductivity  

Science Conference Proceedings (OSTI)

In order to investigate the influence of covalent bonding within the garnet framework on the conductivity of Li+ in the interstitial space, the Li+ conductivities in the family of Sn-based compounds Li6La3 SnMO12 (M = Sb, Nb, Ta) have been obtained and are compared with those of Li6La3ZrMO12. Refinement of the neutron diffraction pattern of Li6La3 SnNbO12shows that the interstitial tetrahedral sites (24d ) are about half-occupied and most of the Li in the interstitial bridging octahedral sites are displaced from the center position (48g ). The Sb-based compound has the largest lattice parameter while the Ta-based compound has the highest Li+-ion conductivity of 0.42 10 4 Scm 1.

Bridges, Craig A [ORNL; Goodenough, J. B. [University of Texas, Austin; Gupta, Dr Asha [University of Texas, Austin; Nakanishi, Masahiro [ORNL; Paranthaman, Mariappan Parans [ORNL; Sokolov, Alexei P [ORNL; Bi, Zhonghe [ORNL; Li, Yutao [University of Texas, Austin; Han, Jiantao [University of Texas, Austin; Dong, Youzhong [South China University of Technology, Guangzhou, PR China; Wang, Long [University of Texas, Austin; Xu, Maowen [University of Texas, Austin

2012-01-01T23:59:59.000Z

383

Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings" (DOE/EA-1918)  

NLE Websites -- All DOE Office Websites (Extended Search)

Environmental Assessment for Final Rule, 10 CFR 433, "Energy Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings" (DOE/EA-1918) June 28, 2013 1 Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings" (DOE/EA-1918) SUMMARY The U.S. Department of Energy (DOE) has prepared this environmental assessment (EA) for DOE's Final Rule, 10 CFR Part 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings". The Final Rule updates the baseline standard in 10 CFR 433 to the latest private sector standard based on cost-effectiveness and DOE's determination that energy efficiency has

384

Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings" (DOE/EA-1918)  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Environmental Assessment for Final Rule, 10 CFR 433, "Energy Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings" (DOE/EA-1918) June 28, 2013 1 Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings" (DOE/EA-1918) SUMMARY The U.S. Department of Energy (DOE) has prepared this environmental assessment (EA) for DOE's Final Rule, 10 CFR Part 433, "Energy Efficiency Standards for New Federal Commercial and Multi-Family High-Rise Residential Buildings". The Final Rule updates the baseline standard in 10 CFR 433 to the latest private sector standard based on cost-effectiveness and DOE's determination that energy efficiency has

385

Validation of a novel expressed sequence tag (EST) clustering method and development of a phylogenetic annotation pipeline for livestock gene families  

E-Print Network (OSTI)

Prediction of functions of genes in a genome is a key step in all genome sequencing projects. Sequences that carry out important functions are likely to be conserved between evolutionarily distant species and can be identified using cross-species comparisons. In the absence of completed genomes and the accompanying high-quality annotations, expressed sequence tags (ESTs) from random cDNA clones are the primary tools for functional genomics. EST datasets are fragmented and redundant, necessitating clustering of ESTs into groups that are likely to have been derived from the same genes. EST clustering helps reduce the search space for sequence homology searching and improves the accuracy of function predictions using EST datasets. This dissertation is a case study that describes clustering of Bos taurus and Sus scrofa EST datasets, and utilizes the EST clusters to make computational function predictions using a comparative genomics approach. We used a novel EST clustering method, TAMUClust, to cluster bovine ESTs and compare its performance to the bovine EST clusters from TIGR Gene Indices (TGI) by using bovine ESTs aligned to the bovine genome assembly as a gold standard. This comparison study reveals that TAMUClust and TGI are similar in performance. Comparisons of TAMUClust and TGI with predicted bovine gene models reveal that both datasets are similar in transcript coverage. We describe here the design and implementation of an annotation pipeline for predicting functions of the Bos taurus (cattle) and Sus scrofa (pig) transcriptomes. EST datasets were clustered into gene families using Ensembl protein family clusters as a framework. Following clustering, the EST consensus sequences were assigned predicted function by transferring annotations of the Ensembl vertebrate protein(s) they are grouped to after sequence homology searches and phylogenetic analysis. The annotations benefit the livestock community by helping narrow down the gamut of direct experiments needed to verify function.

Venkatraman, Anand

2008-12-01T23:59:59.000Z

386

Towards an understanding of protein-protein interaction network hierarchies. Analysis of DnaN (?)-binding peptide motifs in members of protein families interacting with the eubacterial processivity clamp, the ? subunit of DNA Polymerase III  

Science Conference Proceedings (OSTI)

The consensus pentapeptide QL[SD]LF is a major component in the interaction of a number of families of proteins with the eubacterial DNA-clamp protein, DnaN (the ?-subunit of DNA Polymerase III holoenzyme). Rankings of the motifs were established ... Keywords: DNA polymerase III, protein-protein interaction, sliding clamp

Brian P. Dalrymple; Gene Wijffels; Kritaya Kongsuwan; Phil Jennings

2003-01-01T23:59:59.000Z

387

Phase stability, electrochemical stability and ionic conductivity of the Li[subscript 101]MP[subscript 2]X[subscript 12] (M = Ge, Si, Sn, Al or P, and X = O, S or Se) family of superionic conductors  

E-Print Network (OSTI)

We present an investigation of the phase stability, electrochemical stability and Li[superscript +] conductivity of the Li[subscript 101]MP[subscript 2]X[subscript 12] (M = Ge, Si, Sn, Al or P, and X = O, S or Se) family ...

Ong, Shyue Ping

388

Contribution of the PALB2 c.2323C>T [p. Q775X] Founder mutation in well-defined breast and/or ovarian cancer families and unselected ovarian cancer cases of French Canadian descent  

E-Print Network (OSTI)

] mutation carrier fam carrier in family F1469. Abbreviations: bilateral breast cancer (Bi Br), cerebra melanoma (Mel), stomach cancer (Sto), and uterine cancer (Ut). Age at asce diagnosis of cancer. Tischkowitz et al. BMC Medical Genetics 2013, 14:5 Page 4...

Tischkowitz, Marc; Sabbaghian, Nelly; Hamel, Nancy; Pouchet, Carly; Foulkes, William D; Mes-Masson, Anne-Marie; Provencher, Diane M; Tonin, Patricia N

2013-01-09T23:59:59.000Z

389

Crystal structures of MW1337R and lin2004: Representatives of a novel protein family that adopt a four-helical bundle fold  

DOE Green Energy (OSTI)

To extend the structural coverage of proteins with unknown functions, we targeted a novel protein family (Pfam accession number PF08807, DUF1798) for which we proposed and determined the structures of two representative members. The MW1337R gene of Staphylococcus aureus subsp. aureus Rosenbach (Wood 46) encodes a protein with a molecular weight of 13.8 kDa (residues 1-116) and a calculated isoelectric point of 5.15. The lin2004 gene of the nonspore-forming bacterium Listeria innocua Clip11262 encodes a protein with a molecular weight of 14.6 kDa (residues 1-121) and a calculated isoelectric point of 5.45. MW1337R and lin2004, as well as their homologs, which, so far, have been found only in Bacillus, Staphylococcus, Listeria, and related genera (Geobacillus, Exiguobacterium, and Oceanobacillus), have unknown functions and are annotated as hypothetical proteins. The genomic contexts of MW1337R and lin2004 are similar and conserved in related species. In prokaryotic genomes, most often, functionally interacting proteins are coded by genes, which are colocated in conserved operons. Proteins from the same operon as MW1337R and lin2004 either have unknown functions (i.e., belong to DUF1273, Pfam accession number PF06908) or are similar to ypsB from Bacillus subtilis. The function of ypsB is unclear, although it has a strong similarity to the N-terminal region of DivIVA, which was characterized as a bifunctional protein with distinct roles during vegetative growth and sporulation. In addition, members of the DUF1273 family display distant sequence similarity with the DprA/Smf protein, which acts downstream of the DNA uptake machinery, possibly in conjunction with RecA. The RecA activities in Bacillus subtilis are modulated by RecU Holliday-junction resolvase. In all analyzed cases, the gene coding for RecU is in the vicinity of MW1337R, lin2004, or their orthologs, but on a different operon located in the complementary DNA strand. Here, we report the crystal structures of MW1337R and lin2004, which were determined using the semiautomated, high-throughput pipeline of the Joint Center for Structural Genomics (JCSG), part of the National Institute of General Medical Sciences Protein Structure Initiative.

Kozbial, Piotr; Xu, Qingping; Chiu, Hsiu-Ju; McMullan, Daniel; Krishna, S. Sri; Miller, Mitchell D.; Abdubek, Polat; Acosta, Claire; Astakhova, Tamara; Axelrod, Herbert L.; Carlton, Dennis; Clayton, Thomas; Deller, Marc; Duan, Lian; Elias, Ylva; Elsliger, Marc-Andr; Feuerhelm, Julie; Grzechnik, Slawomir K.; Hale, Joanna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Koesema, Eric; Kumar, Abhinav; Marciano, David; Morse, Andrew T.; Murphy, Kevin D.; Nigoghossian, Edward; Okach, Linda; Oommachen, Silvya; Reyes, Ron; Rife, Christopher L.; Spraggon, Glen; Trout, Christina V.; ban den Bedem, Henry; Weekes, Dana; White, Aprilfawn; Wolf, Guenter; Zubieta, Chloe; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A. (Scripps); (SSRL); (JCSG); (UCSD); (Burnham)

2009-08-28T23:59:59.000Z

390

SYNTHESIS OF A NEW FAMILY OF FLUORINATED BORONATE COMPOUNDS AS ANION RECEPTORS AND STUDIES OF THEIR USE AS ADDITIVES IN LITHIUM BATTERY ELECTROLYTES.  

DOE Green Energy (OSTI)

Numerous studies have been done on developing new electrolytes for lithium batteries with high ionic conductivity, and good chemical and electrochemical stability. In addition to the research on new salts and solvents, the use of cation receptors to reduce ion pairing in non-aqueous electrolytes has been considered as an approach to improve the properties of electrolytes. Although both cation and anion receptors enhance the dissociation of ion pairs and increase the conductivity of electrolytes, the use of anion receptors is more attractive for a lithium battery electrolyte because anion receptors increase the lithium transference number in the electrolyte. However, most available neutral anion receptors complex with anions through hydrogen binding and cannot be used in lithium batteries. Recently, we have reported on synthesis of a series of new neutral boron compounds as anion receptors based on the idea that electron-deficient boron would complex the anion of the ion pair. The anion complexation effect of these boron compounds was further enhanced by attaching electron-withdrawing groups. Here we report synthesis of another new family of boronate compounds. The effect of these new compounds on conductivity of lithium salts in non-aqueous solution was studied. The molecular weights of these new boronate compounds are lower than our previously reported boron compounds. Therefore, their effects on conductivity enhancement are superior. They also display high electrochemical stability up to 5 V.

MCBREEN,J.; LEE,H.S.; YANG,X.Q.

2001-06-08T23:59:59.000Z

391

Hsp105 family proteins suppress staurosporine-induced apoptosis by inhibiting the translocation of Bax to mitochondria in HeLa cells  

Science Conference Proceedings (OSTI)

Hsp105 (Hsp105{alpha} and Hsp105{beta}), major heat shock proteins in mammalian cells, belong to a subgroup of the HSP70 family, HSP105/110. Previously, we have shown that Hsp105{alpha} has completely different effects on stress-induced apoptosis depending on cell type. However, the molecular mechanisms by which Hsp105{alpha} regulates stress-induced apoptosis are not fully understood. Here, we established HeLa cells that overexpress either Hsp105{alpha} or Hsp105{beta} by removing doxycycline and examined how Hsp105 modifies staurosporine (STS)-induced apoptosis in HeLa cells. Apoptotic features such as the externalization of phosphatidylserine on the plasma membrane and nuclear morphological changes were induced by the treatment with STS, and the STS-induced apoptosis was suppressed by overexpression of Hsp105{alpha} or Hsp105{beta}. In addition, we found that overexpression of Hsp105{alpha} or Hsp105{beta} suppressed the activation of caspase-3 and caspase-9 by preventing the release of cytochrome c from mitochondria. Furthermore, the translocation of Bax to mitochondria, which results in the release of cytochrome c from the mitochondria, was also suppressed by the overexpression of Hsp105{alpha} or Hsp105{beta}. Thus, it is suggested that Hsp105 suppresses the stress-induced apoptosis at its initial step, the translocation of Bax to mitochondria in HeLa cells.

Yamagishi, Nobuyuki [Department of Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414 (Japan); Ishihara, Keiichi [Department of Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414 (Japan); Saito, Youhei [Department of Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414 (Japan); Hatayama, Takumi [Department of Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto 607-8414 (Japan)]. E-mail: hatayama@mb.kyoto-phu.ac.jp

2006-10-15T23:59:59.000Z

392

The power of the family  

E-Print Network (OSTI)

australia russian federation azerbaijan india italy latviachile algeria armenia azerbaijan spain moldova republic ofrepublic of korea armenia azerbaijan turkey algeria malta

Alesina, Alberto; Giuliano, Paola

2010-01-01T23:59:59.000Z

393

The power of the family  

E-Print Network (OSTI)

south africa bosnia and herzegovina macedonia, republic ofviet nam kyrgyzstan bosnia and herzegovina china austriaislamic republic of) and herzegovina bosnia iran philippines

Alesina, Alberto; Giuliano, Paola

2010-01-01T23:59:59.000Z

394

A Family By Yellow River  

E-Print Network (OSTI)

River, opposite to Shenxi Province across the River, is within the central zone of Huangtu Plateau Culture in midland China. In history Qikou was a transport hinge connecting Sichuan to the west and Baotou (Inner Mongolia) to the northwest. Still seen... . They own seven mu (a mu is one fifteenth of a hectare) of jujube trees, which is an area expanded on a basis of one mu last year. The labour is tough with a typical droughty climate of Loess Plateau. Shouldering a pole with two buckets at either end...

China Central Television (CCTV)

2005-04-06T23:59:59.000Z

395

Metazoan Gene Families from Metazome  

DOE Data Explorer (OSTI)

Metazome is a joint project of the Department of Energy's Joint Genome Institute and the Center for Integrative Genomics to facilitate comparative genomic studies amongst metazoans. Clusters of orthologous and paralogous genes that represent the modern descendents of ancestral gene sets are constructed at key phylogenetic nodes. These clusters allow easy access to clade specific orthology/paralogy relationships as well as clade specific genes and gene expansions. As of version 2.0.4, Metazome provides access to twenty-four sequenced and annotated metazoan genomes, clustered at nine evolutionarily significant nodes. Where possible, each gene has been annotated with PFAM, KOG, KEGG, and PANTHER assignments, and publicly available annotations from RefSeq, UniProt, Ensembl, and JGI are hyper-linked and searchable. The included organisms (by common name) are: Human, Mouse, Rat, Dog, Opossum, Chicken, Frog, Stickleback, Medaka, Fugu pufferfish; Zebrafish, Seasquirt - savignyi, Seasquirt - intestinalis, Amphioxus, Sea Urchin, Fruitfly, Mosquite, Yellow Fever Mosquito, Silkworm, Red Flour Beetle, Worm, Briggsae Worm, Owl limpet (snail), and Sea anemone. [Copied from Metazome Overview at http://www.metazome.net/Metazome_info.php

396

The power of the family  

E-Print Network (OSTI)

OECD, we ?nd that Turkey, Poland, United States, Korea andNetherland, Norway, Poland, Portugal, Sweden, Slovenia,Lithuania Portugal Slovakia Poland Indonesia Philippines

Alesina, Alberto; Giuliano, Paola

2010-01-01T23:59:59.000Z

397

Spec Towns Track Family & Graduate  

E-Print Network (OSTI)

University Village RIVERBEND RD Botany Greenhouses Greenhouses Center for Applied Isotope Study Central Food Barrow Clark Howell Geology Lab Bookstore Reed Milledge Payne Memorial Hall Instructional Plaza Geography/ Geology Georgia Quad WADDELL ST Public Service & Outreach Greenhouses DWBrooksMall Environmental

Hall, Daniel

398

Spec Towns Track Family & Graduate  

E-Print Network (OSTI)

University Village RIVERBEND RD Botany Greenhouses Greenhouses Center for Applied Isotope Study Central Food Barrow Clark Howell Geology Lab Bookstore Reed Milledge Payne Memorial Hall Instructional Plaza Waddel Pharmacy South Performing Arts Geography/ Geology Georgia Quad WADDELL ST Interim Medical

Hall, Daniel

399

Spec Towns Track Family & Graduate  

E-Print Network (OSTI)

University Village RIVERBEND RD Botany Greenhouses Greenhouses Center for Applied Isotope Study Central Food ST SOULE ST WEST GREEN ST DW BROOKSDR Conner Lumpkin House Barrow Clark Howell Geology Lab Bookstore Harris Commons Vandiver Hall WILLIAMSST Waddel Pharmacy South Performing Arts Geography/ Geology Georgia

Hall, Daniel

400

Spec Towns Track Family & Graduate  

E-Print Network (OSTI)

University Village RIVERBEND RD Botany Greenhouses Greenhouses Center for Applied Isotope Study Central Food CEDAR ST SOULE ST WEST GREEN ST DW BROOKSDR Conner Lumpkin House Barrow Clark Howell Geology Lab Harris Commons Vandiver Hall WILLIAMSST Waddel Pharmacy South Performing Arts Geography/ Geology Georgia

Hall, Daniel

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Spec Towns Track Family & Graduate  

E-Print Network (OSTI)

University Village RIVERBEND RD Botany Greenhouses Greenhouses Center for Applied Isotope Study Central Food DW BROOKSDR Conner Lumpkin House Barrow Clark Howell Geology Lab Bookstore Reed Milledge Payne Geography/ Geology Georgia Quad WADDELL ST Interim Medical Partnership Building Public Service & Outreach

Hall, Daniel

402

Work Family Community, Human Resources  

NLE Websites -- All DOE Office Websites (Extended Search)

Work-Life Balance Programs Adoption Assistance Program Benefits Blood Drives Brookhaven Employee's Recreation Association (BERA) Brookhaven Retired Employee Association (BREA)...

403

Sick Leave for Family Purposes  

Science Conference Proceedings (OSTI)

... If you are a part-time employee or on an uncommon tour of duty, the amount you can use will be prorated. Your supervisor ...

2010-10-05T23:59:59.000Z

404

The power of the family  

E-Print Network (OSTI)

with the exception of Greece) are among the countries withFrance, Great Britain, Greece, Hungary, Ireland, Iceland,Poland Indonesia Philippines Greece England Ukraine Spain

Alesina, Alberto; Giuliano, Paola

2010-01-01T23:59:59.000Z

405

Monitoring and evaluation of replacing low-efficiency air conditioners with high-efficiency air conditioners in single-family detached houses in Austin, Texas  

SciTech Connect

The US DOE initiated this project to evaluate the performance of an air conditioner retrofit program in Austin, Texas. The City's Austin's Resource Management Department pursued this project to quantify the retrofit effect of replacing low-efficiency air conditioners with high-efficiency air conditioners in single-family detached homes. If successfully implemented, this retrofit program could help defer construction of a new power plant which is a major goal of this department. The project compares data collected from 12 houses during two cooling seasons under pre-retrofit and then post-retrofit air conditioner units. The existing low-efficiency air conditioners were monitored during the 1987 cooling season, replaced during the 1987--88 heating season with new, smaller sized, high-efficiency units, and then monitored again during the 1988 cooling season. Results indicated that the air conditioner retrofits reduce the annual air conditioner electric consumption and peak electric demand by an average of 38%. When normalized to the nominal capacity of the air conditioner, average demand savings were 1.12 W/ft{sup 2} and estimated annual energy savings were 1.419 kWh/ft{sup 2}. Individual air conditioner power requirements were found to be a well defined function of outdoor temperature as expected. In the absence of detailed data, estimates of the peak demand reductions of new air conditioners can be made from the manufacturer's specifications. Air conditioner energy consumption proved to be strongly linear as a function of the outdoor temperature as expected when taken as an aggregate. No noticeable differences in the diversity factor of the air conditioner usage were found. Analysis of the retrofit effect using PRISM yields estimates of the reduction in normalized annual consumption (NAC) and annual cooling consumption of 12% and 30%. 2 refs., 11 figs., 17 tabs.

Burns, R.; Hough, R.E. (Fleming (W.S.) and Associates, Inc., Syracuse, NY (United States))

1991-10-01T23:59:59.000Z

406

Two families with isolated cat cry without the cri-du-chat syndrome phenotype have an inherited 5p15.3 deletion: Delineation of the larynx malformation region  

SciTech Connect

The cri-du-chat syndrome is a contiguous gene syndrome that results from a deletion of the short arm of chromosome 5 (5p). Patients present with a cat-like cry at birth that is usually considered diagnostic of this syndrome. Additional features of the syndrome include failure to thrive, microcephaly, hypertelorism, epicanthal folds, hypotonia, and severe mental retardation. We report on two families in which the patients with 5p deletions have only the characteristic cat-like cry with normal to mildly delayed development. One family has three children with varying levels of developmental delay and a deletion of 5p15.3 that was inherited from the father. The second family has a mother and daughter both presenting with a cat-like cry and normal intelligence. A de novo deletion in a patient with isolated cat cry and mild developmental delay was also identified. The precise locations of the deletions in each family were determined by fluorescent in situ hybridization using lambda phage, cosmids, and YAC clones. Cryptic translocations and mosaicism were not detected in the parents transmitting the deletion. All of the deletion breakpoints map distal to the previously defined cri-du-chat critical region. A YAC contig has been constructed for the chromosomal region implicated in the larynx malformation. DNA clones mapping in this region will be useful diagnostic tools for delineating 5p deletions that result in the typical features of cri-du-chat syndrome with deletions that result in the isolated cat-like cry feature which is associated with a better prognosis.

Gersh, M.; Overhauser, J. [Thomas Jefferson Univ., Philadelphia, PA (United States); Pasztor, L.M. [Children`s Mercy Hospital, Kansas City, MO (United States)] [and others

1994-09-01T23:59:59.000Z

407

Recommendations for 15% Above-Code Energy Efficiency Measures on Implementing Houston Amendments to Single-Family Buildings in Houston Texas  

E-Print Network (OSTI)

This report presents detailed information about the analysis that was performed to calculate the energy saving potential for residential buildings in Houston. In this analysis the energy efficient measures were proposed by the building officials of the City of Houston. Along with the options proposed by the officials, additional measures were selected from the previously-conducted 15% above code energy analysis conducted by the Energy Systems Laboratory for residential houses across the State of Texas. A total of thirty measures were selected based on the energy savings above the base case. These measures were categorized into five groups: Renewable Power Options, Heating Ventilation and Air Conditioning (HVAC), Fenestration, Envelope and Lighting and Domestic Hot Water (DHW) options. The analysis was performed using a simulation model of an International Energy Conservation Code (IECC)-compliant, single family residence in Houston, Texas. Four sets of simulations were considered based on the choice of heating fuel type and thermostat setback: a) natural gas (i.e., gas-fired furnace for space heating, and gas water heater for domestic water heating) with thermostat setback, b) electricity (i.e., heat pump for space heating, and electric water heater for domestic water heating) with thermostat setback, c) natural gas (i.e., gas-fired furnace for space heating, and gas water heater for domestic water heating) without thermostat setback, and d) electricity (i.e., heat pump for space heating, and electric water heater for domestic water heating) without thermostat setback. Individual measures were then categorized into four groups: 2 to 5%, 5 to 10%, and 10 to 15% and above 15% energy savings above base case. Ten grouped measures were then simulated from combining individual measures from the four categories whose combined savings are more than 15% above the base case. The cost of implementation of the individual as well as grouped measures was also calculated along with a simple payback period. The photovoltaic options presented the maximum savings in the approximate range of 15-40% for all base-case houses. The solar thermal option for domestic water heating presented energy savings above 15-20% for all of the base-case houses.

Liu, Z.; Mukhopadhyay, J.; Malhotra, M.; Kota, S.; Haberl, J. S.; Culp, C.; Yazdani, B.

2008-11-01T23:59:59.000Z

408

Structural and Functional Analysis of Sulfolobus solfataricus Y-Family DNA Polymerase Dpo4-Catalyzed Bypass of the Malondialdehyde?Deoxyguanosine Adduct  

Science Conference Proceedings (OSTI)

Oxidative stress can induce the formation of reactive electrophiles, such as DNA peroxidation products, e.g., base propenals, and lipid peroxidation products, e.g., malondialdehyde. Base propenals and malondialdehyde react with DNA to form adducts, including 3-(2'-deoxy-{beta}-d-erythro-pentofuranosyl)pyrimido[1,2-{alpha}]purin-10(3H)-one (M{sub 1}dG). When paired opposite cytosine in duplex DNA at physiological pH, M{sub 1}dG undergoes ring opening to form N{sup 2}-(3-oxo-1-propenyl)-dG (N{sup 2}-OPdG). Previous work has shown that M{sub 1}dG is mutagenic in bacteria and mammalian cells and that its mutagenicity in Escherichia coli is dependent on induction of the SOS response, indicating a role for translesion DNA polymerases in the bypass of M{sub 1}dG. To probe the mechanism by which translesion polymerases bypass M{sub 1}dG, kinetic and structural studies were conducted with a model Y-family DNA polymerase, Dpo4 from Sulfolobus solfataricus. The level of steady-state incorporation of dNTPs opposite M{sub 1}dG was reduced 260-2900-fold and exhibited a preference for dATP incorporation. Liquid chromatography-tandem mass spectrometry analysis of the full-length extension products revealed a spectrum of products arising principally by incorporation of dC or dA opposite M{sub 1}dG followed by partial or full-length extension. A greater proportion of -1 deletions were observed when dT was positioned 5' of M{sub 1}dG. Two crystal structures were determined, including a 'type II' frameshift deletion complex and another complex with Dpo4 bound to a dC-M{sub 1}dG pair located in the postinsertion context. Importantly, M{sub 1}dG was in the ring-closed state in both structures, and in the structure with dC opposite M{sub 1}dG, the dC residue moved out of the Dpo4 active site, into the minor groove. The results are consistent with the reported mutagenicity of M{sub 1}dG and illustrate how the lesion may affect replication events.

Eoff, Robert L.; Stafford, Jennifer B.; Szekely, Jozsef; Rizzo, Carmelo J.; Egli, Martin; Guengerich, F. Peter; Marnett, Lawrence J.; (Vanderbilt)

2010-01-12T23:59:59.000Z

409

Proteogenomic Analysis of a Thermophilic Bacterial Consortium Adapted to Deconstruct Switchgrass  

SciTech Connect

Thermophilic bacteria are a potential source of enzymes for the deconstruction of lignocellulosic biomass. However, the complement of proteins used to deconstruct biomass and the specific roles of different microbial groups in thermophilic biomass deconstruction are not well-explored. Here we report on the metagenomic and proteogenomic analyses of a compost-derived bacterial consortium adapted to switchgrass at elevated temperature with high levels of glycoside hydrolase activities. Near-complete genomes were reconstructed for the most abundant populations, which included composite genomes for populations closely related to sequenced strains of Thermus thermophilus and Rhodothermus marinus, and for novel populations that are related to thermophilic Paenibacilli and an uncultivated subdivision of the littlestudied Gemmatimonadetes phylum. Partial genomes were also reconstructed for a number of lower abundance thermophilic Chloroflexi populations. Identification of genes for lignocellulose processing and metabolic reconstructions suggested Rhodothermus, Paenibacillus and Gemmatimonadetes as key groups for deconstructing biomass, and Thermus as a group that may primarily metabolize low molecular weight compounds. Mass spectrometry-based proteomic analysis of the consortium was used to identify .3000 proteins in fractionated samples from the cultures, and confirmed the importance of Paenibacillus and Gemmatimonadetes to biomass deconstruction. These studies also indicate that there are unexplored proteins with important roles in bacterial lignocellulose deconstruction.

D'haeseleer, Patrik; Gladden, John M.; Allgaier, Martin; Chain, Patrick; Tringe, Susannah G.; Malfatti, Stephanie; Aldrich, Joshua T.; Nicora, Carrie D.; Robinson, Errol W.; Pasa-Tolic, Ljiljana; Hugenholtz, Philip; Simmons, Blake A.; Singer, Steven W.

2013-07-19T23:59:59.000Z

410

Analyses using Cell Wall Glycan-directed Monoclonal Antibodies Reveal Xylan-degradation by Two Microbial Glycosyl Hydrolases in Cell Walls from Poplar and Switchgrass Biomass  

NLE Websites -- All DOE Office Websites (Extended Search)

ISSN:2155-6199 ISSN:2155-6199 The International Open Access Journal of Bioremediation & Biodegradation Special Issue Title: Biofuels & their applications Handling Editor(s) Kirill I Kostyanovskiy Texas Agri Life Research & Extension Center, USA T his article was originally published in a journal by OMICS Publishing Group, and the attached copy is provided by OMICS Publishing Group for the author's benefit and for the benefit of the author's institution, for commercial/research/educational use including without limitation use in instruction at your institution, sending it to specific colleagues that you know, and providing a copy to your institution's administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access,

411

Structure and magnetic properties of the Al{sub 1-x}Ga{sub x}FeO{sub 3} family of oxides: A combined experimental and theoretical study  

Science Conference Proceedings (OSTI)

Magnetic properties of the Al{sub 1-x}Ga{sub x}FeO{sub 3} family of oxides crystallizing in a non-centrosymmetric space group have been investigated in detail along with structural aspects by employing X-ray and neutron diffraction, Moessbauer spectroscopy and other techniques. The study has revealed the occurrence of several interesting features related to unit cell parameters, site disorder and ionic size. Using first-principles density functional theory based calculations, we have attempted to understand how magnetic ordering and related properties in these oxides depend sensitively on disorder at the cation site. The origin and tendency of cations to disorder and the associated properties are traced to the local structure and ionic sizes. -- Graphical abstract: We have studied both experimentally and theoretically the important role of disorder at the cation site on magnetic and related properties of the Al{sub 1-x}Ga{sub x}FeO{sub 3} family of oxides crystallizing in a non-centrosymmetric space group. Display Omitted Research highlights: {yields} Interesting observations on cation site disorder, cell parameters and ionic size. {yields} Cation site disorder explains magnetic ordering. {yields} Demonstrates the importance of the A-site cations.

Saha, Rana; Shireen, Ajmala [Chemistry and Physics of Materials Unit, New Chemistry Unit, Theoretical Science Unit and International Centre for Materials Science, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064 (India); Bera, A.K. [Bhabha Atomic Research Centre, Trombay, Mumbai 400 085 (India); Shirodkar, Sharmila N.; Sundarayya, Y.; Kalarikkal, Nandakumar [Chemistry and Physics of Materials Unit, New Chemistry Unit, Theoretical Science Unit and International Centre for Materials Science, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064 (India); Yusuf, S.M. [Bhabha Atomic Research Centre, Trombay, Mumbai 400 085 (India); Waghmare, Umesh V. [Chemistry and Physics of Materials Unit, New Chemistry Unit, Theoretical Science Unit and International Centre for Materials Science, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064 (India); Sundaresan, A., E-mail: sundaresan@jncasr.ac.i [Chemistry and Physics of Materials Unit, New Chemistry Unit, Theoretical Science Unit and International Centre for Materials Science, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064 (India); Rao, C.N.R, E-mail: cnrrao@jncasr.ac.i [Chemistry and Physics of Materials Unit, New Chemistry Unit, Theoretical Science Unit and International Centre for Materials Science, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064 (India)

2011-03-15T23:59:59.000Z

412

Draft Environmental Assessment for Direct Final Rule, 10 CFR 434, "Energy Standards for New Federal Commercial and High-Rise High-Rise Multi-FamilyResidential Buildings" and 10 CFR 435, "Energy Efficiency Standards for New Federal Residential Low-Rise Re  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

"Energy "Energy Efficiency Standards for New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings" Baseline Standards Update (DOE/EA-1871) March 16, 2011 2 Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings" Baseline Standards Update

413

Genome sequence of the flexirubin-pigmented soil bacterium Niabella soli type strain (JS13-8T)  

SciTech Connect

Niabella soli Weon et al. 2008 is a member of the Chitinophagaceae, a family within the class Sphingobacteriia that is poorly characterized at the genome level, thus far. N. soli strain JS13-8T is of interest for its ability to produce a variety of glycosyl hydrolases. The ge- nome of N. soli strain JS13-8T is only the second genome sequence of a type strain from the family Chitinophagaceae to be published, and the first one from the genus Niabella. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,697,343 bp long chromosome with its 3,931 protein-coding and 49 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Anderson, Iain [U.S. Department of Energy, Joint Genome Institute; Munk, Christine [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Tindall, Brian [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Ivanova, N [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

414

Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum  

Science Conference Proceedings (OSTI)

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

Adney, William S.; Baker, John O.; Decker, Stephen R.; Chou, Yat-Chen; Himmel, Michael E.; Ding, Shi-You

2012-10-09T23:59:59.000Z

415

Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum  

DOE Patents (OSTI)

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

Adney, William S. (Golden, CO); Baker, John O. (Golden, CO); Decker, Stephen R. (Berthoud, CO); Chou, Yat-Chen (Golden, CO); Himmel, Michael E. (Littleton, CO); Ding, Shi-You (Golden, CO)

2008-11-11T23:59:59.000Z

416

20001:1:obama family tree  

Science Conference Proceedings (OSTI)

... 20959:1:african american. 20960:1:pest control education. 20961:1:compare salaries. 20962:1:"diy+audio". 20963:1:candlestick park earthquake. ...

417

Generalized statistical methods for mixed exponential families  

E-Print Network (OSTI)

data set . . . . . . . . . . . . . . . . . . . . . . . cate-Table D.21 Abalone data set: attributesNewsgroups data set . . . . . . . . . . . . . . . . . D.1.1

Levasseur, Ccile

2009-01-01T23:59:59.000Z

418

An Activity Book For African American Families  

E-Print Network (OSTI)

. It represents the kind of public-private partnership that uses the strengths of all partners to improve

Rau, Don C.

419

Welcome,Families Oldest and Largest  

E-Print Network (OSTI)

Sports 32 #12;Page 2 THE TECH WORLD & NATION October 18, 2002 Vatican Opposes U.S. Church's Zero Tolerance Sex Abuse Rule North Korea Nuke Program Caught By U.S. Intelligence Six Palestinians Die in Gaza electricity prices, the first criminal charges stemming from the power crisis there two years ago. Timothy

420

Research Brief Children, Families, and the Criminal  

E-Print Network (OSTI)

children in the United States are currently affected by parental involvement in the correctional system St., Chicago, Illinois 60607 Phone: 312-413-2302 Fax: 312-996-1802 http disorganized lives marked by drug addiction, irresponsible actions, and high- risk lifestyles. Several adult

Illinois at Chicago, University of

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

XAX1 from glycosyltransferase family 61 mediates ...  

components of human and animal nutrition, constituting a major component of dietary ?ber in cereals (1). The amount of xylan as ... Error bars are SEM ...

422

Studies on the Cave- Spider Family Leptonetidae  

E-Print Network (OSTI)

Cave, Comal County (AMNH), Texas, habitus. A. T. coecas Cave, Comal County, Texas (AMNH), habitus. A. T. coecaLost Gold Cave, Travis County, Texas (AMNH), habitus. A. T.

Ledford, Joel M.

2011-01-01T23:59:59.000Z

423

Work-Family Conflict and Retirement Preferences  

E-Print Network (OSTI)

and Variable Descriptions: 1992 Wisconsin Longitudinal StudyStatus in Ten Years: 1992 Wisconsin Longitudinal Study (n =Sweeney 2 1: University of Wisconsin-Madison, Department of

Raymo, James M.; Sweeney, Megan M

2005-01-01T23:59:59.000Z

424

Characterization of paralogous protein families in rice  

E-Print Network (OSTI)

Background: High gene numbers in plant genomes reflect polyploidy and major gene duplication events. Oryza sativa, cultivated rice, is a diploid monocotyledonous species with a ~390 Mb genome that has undergone segmental ...

Lin, Haining

425

Families of p?-adic Galois representations  

E-Print Network (OSTI)

In this thesis, I first generalize Kisin's theory of finite slope subspaces to arbitrary p-adic fields, and then apply it to the generic fibers of Galois deformation spaces. I study the finite slope deformation rings in ...

Tan, Fucheng, Ph. D. Massachusetts Institute of Technology

2011-01-01T23:59:59.000Z

426

University of Southern California Mork Family  

E-Print Network (OSTI)

remarks 10:10 AM research talks 10:10 AM Hong Seok Choi Polymer-coated silica ultra-high-Q microresonators Tsotsis) 11:40 AM Ting-Wei Yeh GaN nanorod arrays for efficient LEDs grown by selective area growth (with Graduate student posters Jingran Ma Real-time model predictive control for energy and demand optimization

Southern California, University of

427

Unmarried cohabitation among deprived families in Chile  

E-Print Network (OSTI)

% 80% 90% 100% Chile Uruguay Mexico Costa Rica Argentina Bolivia Paraguay Brazil Ecuador Guatemala Belize Cuba Peru Venezuela Nicaragua El Salvador Colombia Panama Honduras Dominican Republic Unmarried Cohabitation Marriage Data sources: For all... of all unions. This group is formed by Venezuela, Peru, Cuba, Belize, Guatemala. Finally, the countries where unmarried cohabitation represents no more than one third of total unions are geographically located in the outer circle.2 Thus, the countries...

Ramm Santelices, Alejandra Margarita

2013-06-12T23:59:59.000Z

428

The extended family of hexagonal molybdenum oxide  

SciTech Connect

Over the last 40 years, a large number of isostructural compounds in the system MoO{sub 3}-NH{sub 3}-H{sub 2}O have been published. The reported molecular formulae of 'hexagonal molybdenum oxide' (HEMO) varied from MoO{sub 3}, MoO{sub 3} {center_dot} 0.33NH{sub 3}, MoO{sub 3} {center_dot} nH{sub 2}O (0.09 {le} n {le} 0.69) to MoO{sub 3} {center_dot} mNH{sub 3} {center_dot} nH{sub 2}O (0.09 {le} m {le} 0.20; 0.18 {le} n {le} 0.60). Samples, prepared by the acidification route, were investigated using thermal analysis coupled on-line to a mass spectrometer for evolved gas analysis; X-ray powder diffraction; Fourier Transform Infrared, Raman and Magic-Angle-Spinning {sup 1}H-NMR spectroscopy; Incoherent Inelastic Neutron Scattering. The X-ray study of a selected monocrystal confirmed the presence of the well-known framework of edge-sharing MoO{sub 6} octahedra: Space group P6{sub 3}/m, a = 10.527(1), c =3.7245(7) {angstrom}, {gamma} = 120{sup o}. The structure of the synthesized samples can best be described by the structural formula (NH{sub 4})[Mo{sub x}{open_square}{sub 1/2+p/2}(O{sub 3x + 1/2-p/2})(OH){sub p}] {center_dot} yH{sub 2}O (x 5.9-7.1; p {approx} 0.1; y = 1.2-2.6), which is consistent with the existence of one vacancy for 12-15 molybdenum sites. The 'chimie douce' reaction of MoO{sub 3} {center_dot} 0.155NH{sub 3} {center_dot} 0.440H{sub 2}O with a 1:1 mixture of NO/NO{sub 2} at 100 C resulted in the synthesis of MoO{sub 3} {center_dot} 0.539H{sub 2}O. Tailored nano-sized molybdenum powders can be produced using HEMO as precursor.

Hartl, Monika [Los Alamos National Laboratory; Daemen, Luke [Los Alamos National Laboratory; Lunk, J H [NON LANL; Hartl, H [NON LANL; Frisk, A T [NON LANL; Shendervich, I [NON LANL; Mauder, D [NON LANL; Feist, M [NON LANL; Eckelt, R [NON LANL

2009-01-01T23:59:59.000Z

429

World Catalog of the Family Tethinidae (Diptera)  

E-Print Network (OSTI)

specimens (10 males, 7 females) of C. herbacea from the area of Bcharré/Les Cedres at Mount Lebanon; "Zorkun, 1500-1600 m, Aksaray prov., Turkey). 5. C. herbacea ("Bcharré"; Bcharré/Les Cedres, Lebanon). 6. C pass, 2600 m, 19.7.1987, Heinz leg. (2MM, 2WW)*. Cicindela herbacea ­ Lebanon: Bcharré, Les Cedres, 6

Mathis, Wayne N.

430

WHAT'SINSIDE? Dear Trojan Family.......................................................  

E-Print Network (OSTI)

in today's program exemplify the innovative spirit of USC students. The USC Student Innovator Showcase gives parents and students a sneak peek at tomorrow's world. Today's program highlights some of the most participating. (In this program, the number on each presenters page corresponds to their location

Southern California, University of

431

Mara Ins Cataln: Cataln Family Farm  

E-Print Network (OSTI)

My son, you have to put compost on it, and dig more, and mixtheir bucket full of compost. We have furrows and furrowshijo tienes que ponerle compost, escarbar mas, revolver bien

Reti, Irene H.

2010-01-01T23:59:59.000Z

432

Mara Ins Cataln: Cataln Family Farm  

E-Print Network (OSTI)

an organic farming training program at the Rural DevelopmentLand- Based Training Association program). This incubatorprogram director for the Agriculture & Land-Based Training

Reti, Irene H.

2010-01-01T23:59:59.000Z

433

Leaking Pipelines: Doctoral Student Family Formation  

E-Print Network (OSTI)

Sari M. Why the Academic Pipeline Leaks: Fewer Men thanone reason the academic pipeline leaks. 31 Blair-Loy, Mary.to leak out of the academic pipeline. The term academic

Serrano, Christyna M.

2008-01-01T23:59:59.000Z

434

Evolution of the Oligopeptide Transporter (OPT) family  

E-Print Network (OSTI)

evaluation. Name Publication NF-kB Tian et al. 2005a 173expressed mutant IkB blocking NF-kB signaling (labeled based selection in (a) NF-kB dataset, (b) ALL dataset, (c)

Gomolplitinant, Kenny Matee

2010-01-01T23:59:59.000Z

435

Parents and Families LEHIGH UNIVERSITY 1 Anonymous ++  

E-Print Network (OSTI)

E. Decker `33* 1934 President Associates Elinor and S. Murray* Rust, Jr. `34 `61P `67P `72P ++ Jane F. and Clark K. Riley `57 ++ Helene M. and Anton Roeger III `57 Jill E. and C. Keith Rust `57 `82P

Gilchrist, James F.

436

Chicagoland Single-Family Housing Characterization  

Science Conference Proceedings (OSTI)

In this report, the PARR team identifies housing characteristics and energy use for fifteen housing types (groups) in the Chicagoland (Cook County, Illinois) region and specifies measure packages that provide an optimum level of energy savings based on a BEopt analysis. The analysis is based on assessor data and actual energy consumption data on 432,605 houses representing approximately 30% of the population.

Spanier, J.; Scheu, R.; Brand, L.; Yang, J.

2012-06-01T23:59:59.000Z

437

California Immigrant Families: Issues for California's Future  

E-Print Network (OSTI)

F O R N I A CPRC Brief CALIFORNIA PROGRAM ON ACCESS TO CAREFamilies: Issues for Californias Future Public Forumsin the United States lives in California, few studies have

Research Center, California Policy; Health Policy Research, UCLA Center for

2000-01-01T23:59:59.000Z

438

Environmental Assessment for Direct Final Rule, 10 CFR 434, Energy Standards for New Federal Commercial and High-Rise Multi-FamilyResidential BuildingsŽ and 10 CFR 435, Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings"  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Proposed Rule, 10 CFR 433, Proposed Rule, 10 CFR 433, "Sustainable Design and Energy Efficiency Standards for New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Sustainable Design and Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings" (DOE/EA-1463) 2 SUMMARY The U.S. Department of Energy (DOE) has prepared this Environmental Assessment (EA) for DOE's Proposed Rule, 10 CFR 433, "Sustainable Design and Energy Efficiency Standards for the Design and Construction of New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Sustainable Design and Energy Efficiency Standards for New Federal Residential Low- Rise Residential Buildings". Section 305(a) of the Energy Conservation and Production

439

Draft Environmental Assessment for Proposed Rule, 10 CFR Part 433, Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Commercial and Multi-Family High-Rise Residential BuildingsŽ  

NLE Websites -- All DOE Office Websites (Extended Search)

Draft Environmental Assessment for Proposed Rule, 10 CFR Part 433, Draft Environmental Assessment for Proposed Rule, 10 CFR Part 433, "Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Commercial and Multi-Family High-Rise Residential Buildings" and 10 CFR Part 435 "Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Low-Rise Residential Buildings" (DOE/EA-1778) 2 SUMMARY The U.S. Department of Energy (DOE) has prepared this Environmental Assessment (EA) for DOE's Proposed Rule, 10 CFR Part 433, "Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Commercial and Multi-Family High-Rise Residential

440

Environmental Assessment for Direct Final Rule, 10 CFR 434, Energy Standards for New Federal Commercial and High-Rise Multi-FamilyResidential BuildingsŽ and 10 CFR 435, Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings"  

NLE Websites -- All DOE Office Websites (Extended Search)

Proposed Rule, 10 CFR 433, Proposed Rule, 10 CFR 433, "Sustainable Design and Energy Efficiency Standards for New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Sustainable Design and Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings" (DOE/EA-1463) 2 SUMMARY The U.S. Department of Energy (DOE) has prepared this Environmental Assessment (EA) for DOE's Proposed Rule, 10 CFR 433, "Sustainable Design and Energy Efficiency Standards for the Design and Construction of New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Sustainable Design and Energy Efficiency Standards for New Federal Residential Low- Rise Residential Buildings". Section 305(a) of the Energy Conservation and Production

Note: This page contains sample records for the topic "glycoside hydrolase family" from the National Library of EnergyBeta (NLEBeta).
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they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
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441

Draft Environmental Assessment for Proposed Rule, 10 CFR Part 433, Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Commercial and Multi-Family High-Rise Residential BuildingsŽ  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Draft Environmental Assessment for Proposed Rule, 10 CFR Part 433, Draft Environmental Assessment for Proposed Rule, 10 CFR Part 433, "Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Commercial and Multi-Family High-Rise Residential Buildings" and 10 CFR Part 435 "Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Low-Rise Residential Buildings" (DOE/EA-1778) 2 SUMMARY The U.S. Department of Energy (DOE) has prepared this Environmental Assessment (EA) for DOE's Proposed Rule, 10 CFR Part 433, "Energy Conservation and Fossil Fuel-Generated Energy Consumption Reduction Standards for the Design and Construction of New Federal Commercial and Multi-Family High-Rise Residential

442

Draft Environmental Assessment for Direct Final Rule, 10 CFR 434, Energy Standards for New Federal Commercial and High-Rise High-Rise Multi-FamilyResidential BuildingsŽ and 10 CFR 435, Energy Efficiency Standards for New Federal Residential Low-Rise Re  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

7 7 Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings" (DOE/EA-1463) Environmental Assessment for Final Rule, 10 CFR 433, "Energy Efficiency Standards for New Federal Commercial and High-Rise Multi-Family Residential Buildings" and 10 CFR 435, "Energy Efficiency Standards for New Federal Residential Low-Rise Residential Buildings" (DOE/EA-1463) SUMMARY The U.S. Department of Energy (DOE) has prepared this Environmental Assessment (EA) for DOE's Final Rule, 10 CFR 433, "Energy Efficiency Standards for

443

Complete genome of the cellulolytic thermophile Acidothermus cellulolyticus 11B provides insights into its ecophysiological and evolutionary adaptations  

Science Conference Proceedings (OSTI)

We present here the complete 2.4 MB genome of the actinobacterial thermophile, Acidothermus cellulolyticus lIB, that surprisingly reveals thermophilic amino acid usage in only the cytosolic subproteome rather than its whole proteome. Thermophilic amino acid usage in the partial proteome implies a recent, ongoing evolution of the A. cellulolyticus genome since its divergence about 200-250 million years ago from its closest phylogenetic neighbor Frankia, a mesophilic plant symbiont. Differential amino acid usage in the predicted subproteomes of A. cellulolyticus likely reflects a stepwise evolutionary process of modern thermophiles in general. An unusual occurrence of higher G+C in the non-coding DNA than in the transcribed genome reinforces a late evolution from a higher G+C common ancestor. Comparative analyses of the A. cellulolyticus genome with those of Frankia and other closely-related actinobacteria revealed that A. cellulolyticus genes exhibit reciprocal purine preferences at the first and third codon positions, perhaps reflecting a subtle preference for the dinucleotide AG in its mRNAs, a possible adaptation to a thermophilic environment. Other interesting features in the genome of this cellulolytic, hot-springs dwelling prokaryote reveal streamlining for adaptation to its specialized ecological niche. These include a low occurrence of pseudogenes or mobile genetic elements, a flagellar gene complement previously unknown in this organism, and presence of laterally-acquired genomic islands of likely ecophysiological value. New glycoside hydrolases relevant for lignocellulosic biomass deconstruction were identified in the genome, indicating a diverse biomass-degrading enzyme repertoire several-fold greater than previously characterized, and significantly elevating the industrial value of this organism.

Xie, Gary [Los Alamos National Laboratory; Detter, Chris [Los Alamos National Laboratory; Bruce, David [Los Alamos National Laboratory; Challacome, Jean F [Los Alamos National Laboratory; Brettin, Thomas S [Los Alamos National Laboratory; Barabote, Ravi D [UC DAVIS; Leu, David [UC DAVIS; Normand, Philippe [CNRS, UNIV LYON; Necsula, Anamaria [CNRS, UNIV LYON; Daubin, Vincent [CNRS, UNIV LYON; Medigue, Claudine [CNRS/GENOSCOPE; Adney, William S [NREL; Xu, Xin C [UC DAVIS; Lapidus, Alla [DOE JOINT GENOME INST.; Pujic, Pierre [CNRS, UNIV LYON; Richardson, Paul [DOE JOINT GENOME INST; Berry, Alison M [UC DAVIS

2008-01-01T23:59:59.000Z

444

Complete genome of the cellulolytic thermophile Acidothermus cellulolyticus 11B provides insights into its ecophysiological and evolutionary adaptations  

Science Conference Proceedings (OSTI)

We present here the complete 2.4 MB genome of the actinobacterial thermophile, Acidothermus cellulolyticus 11B, that surprisingly reveals thermophilic amino acid usage in only the cytosolic subproteome rather than its whole proteome. Thermophilic amino acid usage in the partial proteome implies a recent, ongoing evolution of the A. cellulolyticus genome since its divergence about 200-250 million years ago from its closest phylogenetic neighbor Frankia, a mesophilic plant symbiont. Differential amino acid usage in the predicted subproteomes of A. cellulolyticus likely reflects a stepwise evolutionary process of modern thermophiles in general. An unusual occurrence of higher G+C in the non-coding DNA than in the transcribed genome reinforces a late evolution from a higher G+C common ancestor. Comparative analyses of the A. cellulolyticus genome with those of Frankia and other closely-related actinobacteria revealed that A. cellulolyticus genes exhibit reciprocal purine preferences at the first and third codon positions, perhaps reflecting a subtle preference for the dinucleotide AG in its mRNAs, a possible adaptation to a thermophilic environment. Other interesting features in the genome of this cellulolytic, hot-springs dwelling prokaryote reveal streamlining for adaptation to its specialized ecological niche. These include a low occurrence of pseudo genes or mobile genetic elements, a flagellar gene complement previously unknown in this organism, and presence of laterally-acquired genomic islands of likely ecophysiological value. New glycoside hydrolases relevant for lignocellulosic biomass deconstruction were identified in the genome, indicating a diverse biomass-degrading enzyme repertoire several-fold greater than previously characterized, and significantly elevating the industrial value of this organism.

Xie, Gary [Los Alamos National Laboratory; Detter, John C [Los Alamos National Laboratory; Bruce, David C [Los Alamos National Laboratory; Challacombe, Jean F [Los Alamos National Laboratory; Brettin, Thomas S [Los Alamos National Laboratory; Necsulea, Anamaria [UNIV LYON; Daubin, Vincent [UNIV LYON; Medigue, Claudine [GENOSCOPE; Adney, William S [NREL; Xu, Xin C [UC DAVIS; Lapidus, Alla [JGI; Pujic, Pierre [UNIV LYON; Berry, Alison M [UC DAVIS; Barabote, Ravi D [UC DAVIS; Leu, David [UC DAVIS; Normand, Phillipe [UNIV LYON

2009-01-01T23:59:59.000Z

445

2007 GRC on Cellulases and Cellulosomes (July 29-August 3, 2007)  

DOE Green Energy (OSTI)

Cellulose, a key component of the plant cell wall, comprises the most abundant source of organic carbon on the planet and its microbial degradation is of considerable biological and industrial importance. Indeed, the microbial degradation of cellulose and other plant structural polysaccharides is critical to the maintenance of the carbon cycle in terrestrial and marine microbial ecosystems, host invasion by several phytopathogens, and herbivore nutrition. While the enzymes that attack cellulose are already widely used in several biotechnology-based industries, the major future application of these biocatalysts is the conversion of plant biomass into bio-ethanol and other forms of energy. In that context, the 2007 Conference will present the latest breakthroughs in our understanding of the enzymology, structural biology and (meta)genomics underpinning the conversion of plant structural polysaccharides into fermentable sugars, both in natural and engineered processes. There is also an increased emphasis on the roles of other carbohydrate active enzymes in plant biomass conversion. The themes for the scientific sessions include: (1) crystallographic and biochemical analyses of enzyme structure and function; (2) molecular mechanisms underpinning enzyme catalysis, processivity and specificity; (3) functional and comparative analyses of carbohydrate binding modules and enzyme-substrate interactions; (4) directed evolution for the development of catalytically superior glycoside hydrolases; (5) biophysical and structural analyses of native and chemically pretreated plant biomass and component polysaccharides; (6) genomics of specialist polysaccharide degrading microbes; (7) metagenomics and ecophysiology of plant biomass degradation in natural and engineered processes and; (8) enhancement of industrial bioprocesses by metabolic engineering and related approaches. While the Conference draws many of its participants from academia and government agencies, colleagues from industry have made many important and valuable contributions to the success of all the Conferences. This makes the Conference a truly interactive and productive venue for all sectors interested in the fundamental and applied sciences of cellulases, cellulosomes, and other carbohydrate active enzymes.

Mark Morrison

2008-09-22T23:59:59.000Z

446

Genome, transcriptome, and secretome analysis of wood decay fungus postia placenta supports unique mechanisms of lignocellulose conversion  

SciTech Connect

Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative {beta}-1-4 endoglucanase were expressed at high levels relative to glucose grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC{center_dot}MSIMS). Also upregulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H202. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H202 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons to the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.

Martinez, Diego [Los Alamos National Laboratory; Challacombe, Jean F [Los Alamos National Laboratory; Misra, Monica [Los Alamos National Laboratory; Xie, Gary [Los Alamos National Laboratory; Brettin, Thomas [Los Alamos National Laboratory; Morgenstern, Ingo [CLARK UNIV; Hibbett, David [CLARK UNIV.; Schmoll, Monika [UNIV WIEN; Kubicek, Christian P [UNIV WIEN; Ferreira, Patricia [CIB, CSIC, MADRID; Ruiz - Duenase, Francisco J [CIB, CSIC, MADRID; Martinez, Angel T [CIB, CSIC, MADRID; Kersten, Phil [FOREST PRODUCTS LAB; Hammel, Kenneth E [FOREST PRODUCTS LAB; Vanden Wymelenberg, Amber [U. WISCONSIN; Gaskell, Jill [FOREST PRODUCTS LAB; Lindquist, Erika [DOE JGI; Sabati, Grzegorz [U. WISCONSIN; Bondurant, Sandra S [U. WISCONSIN; Larrondo, Luis F [U. CATHOLICA DE CHILE; Canessa, Paulo [U. CATHOLICA DE CHILE; Vicunna, Rafael [U. CATHOLICA DE CHILE; Yadavk, Jagiit [U. CINCINATTI; Doddapaneni, Harshavardhan [U. CINCINATTI; Subramaniank, Venkataramanan [U. CINCINATTI; Pisabarro, Antonio G [PUBLIC U. NAVARRE; Lavin, Jose L [PUBLIC U. NAVARRE; Oguiza, Jose A [PUBLIC U. NAVARRE; Master, Emma [U. TORONTO; Henrissat, Bernard [CNRS, MARSEILLE; Coutinho, Pedro M [CNRS, MARSEILLE; Harris, Paul [NOVOZYMES, INC.; Magnuson, Jon K [PNNL; Baker, Scott [PNNL; Bruno, Kenneth [PNNL; Kenealy, William [MASCOMA, INC.; Hoegger, Patrik J [GEORG-AUGUST-U.; Kues, Ursula [GEORG-AUGUST-U; Ramaiva, Preethi [NOVOZYMES, INC.; Lucas, Susan [DOE JGI; Salamov, Asaf [DOE JGI; Shapiro, Harris [DOE JGI; Tuh, Hank [DOE JGI; Chee, Christine L [UNM; Teter, Sarah [NOVOZYMES, INC.; Yaver, Debbie [NOVOZYMES, INC.; James, Tim [MCMASTER U.; Mokrejs, Martin [CHARLES U.; Pospisek, Martin [CHARLES U.; Grigoriev, Igor [DOE JGI; Rokhsar, Dan [DOE JGI; Berka, Randy [NOVOZYMES; Cullen, Dan [FOREST PRODUCTS LAB

2008-01-01T23:59:59.000Z

447

Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30  

Science Conference Proceedings (OSTI)

Development of alternative, non-petroleum based sources of bioenergy that can be applied in the short-term find great promise in the use of highly abundant and renewable lignocellulosic plant biomass.1 This material obtained from different feedstocks, such as forest litter or agricultural residues, can yield liquid fuels and other chemical products through biorefinery processes.2 Biofuels are obtained from lignocellulosic materials by chemical pretreatment of the biomass, followed by enzymatic decomposition of cellulosic and hemicellulosic compounds into soluble sugars that are converted to desired chemical products via microbial metabolism and fermentation.3, 4 To release soluble sugars from polymeric cellulose multiple enzymes are required, including endoglucanase, exoglucanase, and ?-glucosidase.5, 6 However, the enzymatic hydrolysis of cellulose into soluble sugars remains a significant limiting factor to the efficient and economically viable utilization of lignocellulosic biomass for transport fuels.7, 8 The primary industrial source of cellulose and hemicellulases is the mesophilic soft-rot fungus Trichoderma reesei,9 having widespread applications in food, feed, textile, pulp, and paper industries.10 The genome encodes 200 glycoside hydrolases, including 10 cellulolytic and 16 hemicellulolytic enzymes.11 The hypercellulolytic catabolite derepressed strain RUT-C30 was obtained through a three-step UV and chemical mutagenesis of the original T. reesei strain QM6a,12, 13 in which strains M7 and NG14 were intermediate, having higher cellulolytic activity than the parent strain but less activity and higher catabolite repression than RUT-C30.14 Numerous methods have been employed to optimize the secreted enzyme cocktail of T. reesei including cultivation conditions, operational parameters, and mutagenesis.3 However, creating an optimal and economical enzyme mixture for production-scale biofuels synthesis may take thousands of experiments to identify.

Anderson, Lindsey N.; Culley, David E.; Hofstad, Beth A.; Chauvigne-Hines, Lacie M.; Zink, Erika M.; Purvine, Samuel O.; Smith, Richard D.; Callister, Stephen J.; Magnuson, Jon M.; Wright, Aaron T.

2013-12-01T23:59:59.000Z

448

Families created by gamete donation: disclosure and family functioning when children are seven years old  

E-Print Network (OSTI)

raises the child. Donor insemination (DI) is one of the oldest techniques in reproductive medicine and is a relatively straightforward procedure in which semen is transferred to the vagina via syringe. This typically takes place within a clinic so...

Blake, Lucy

2012-02-07T23:59:59.000Z

449

Family Nutrition In Action Family Nutrition Program Vol. 6 No. 6  

E-Print Network (OSTI)

. . . . . . . . . . . . . . . . chicken broth 1 small . tart red apple, cored & chopped 3 tablespoons . . . . . . . . chopped pecans 1 shopping or ride a stationary bicycle during your favorite TV show. Plan holiday meals - Try low-fat% or non-fat milk in recipes, or use fat-free whipped toppings. Social events - Decide which parties you

Florida, University of

450

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June  

NLE Websites -- All DOE Office Websites (Extended Search)

0, 0, p. 3545-3553 Vol. 76, No. 11 0099-2240/10/$12.00 doi:10.1128/AEM.02689-09 Copyright © 2010, American Society for Microbiology. All Rights Reserved. Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost ᰔ Javier A. Izquierdo, 1,2 Maria V. Sizova, 1,2 † and Lee R. Lynd 1,2 * Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 Received 4 November 2009/Accepted 30 March 2010 The enrichment from nature of novel microbial communities with high cellulolytic activity is useful in the identification of novel organisms and novel functions that enhance the fundamental understanding of micro- bial cellulose degradation. In this work we identify predominant organisms in three cellulolytic

451

Detailed Analysis of Thermal Mass Effects in a Code-Traceable DOE-2 Simulation of the 2000 IECC for a Single-Family Residence in Texas: A Project for Texas' Senate Bill 5 Legislation for Reducing Pollution in Nonattainment and Affected Areas  

E-Print Network (OSTI)

This study examines the thermal mass effects in a code-traceable DOE-2 simulation of the 2000 IECC (International Energy Conservation Code) for a single-family residence in Texas. This report is composed of two major simulations: 1) the simulation according to the location of the insulation of IECC2000, and 2) the simulation according to the types of real brick and block walls which are practically used at the residential house. In this study, the 2000 IECC was used to develop the base case simulation model in Houston, Texas. The DOE-2 energy simulation program was used to analyze changes to the annual energy use caused by changing various building materials. The best energy conservative material layout was then chosen that contained reduced annual energy use, peak cooling and heating loads, and peak day electricity use.

Kim, S.; Haberl, J. S.

2008-07-18T23:59:59.000Z

452

Synthesis and structure of a new family of 3d-4f heterometallic compounds Rb{sub 7}LnFe{sub 6}O{sub 2}(PO{sub 4}){sub 8} (Ln=Sm, Eu, Gd, Dy): Magnetic properties of the Sm-, Gd-, Dy-derivatives  

SciTech Connect

A new family of mixed lanthanide(III) and iron(III) oxo-phosphate phases, Rb{sub 7}LnFe{sub 6}O{sub 2}(PO{sub 4}){sub 8} (Ln=Sm 1, Eu 2, Gd 3, Dy 4), was isolated by using a high-temperature, solid-state method in molten-salt media. The X-ray single-crystal structure analysis shows that these isomorphic derivatives crystallize in a triclinic space group P-1 (no. 2); Z=1. The 3-D framework of these 3d-4f oxo-phosphates are comprised of LnO{sub 6} octahedral, FeO{sub 5} trigonal bipyramidal (tbp), and {mu}{sub 3}-oxo [Fe{sub 4}O{sub 18}] tetrameric units interconnected through PO{sub 4} tetrahedra. The preliminary results of the temperature-dependent magnetic susceptibility measurements for selected compounds (1, 3, 4) reveal antiferromagnetic-like behavior. 1 shows a weak antiferromagnetric ordering at T{sub N}={approx}7 K while others show little evidence of long-range magnetic order down to 2 K. All three compounds have measured magnetic moments significantly smaller than the expected values. - Graphical abstract: The extended framework is described by the connectivity of three distinct types of paramagnetic units. The temperature-dependent, lower than expected effective magnetic moments are reported. Highlights: Black-Right-Pointing-Pointer A new family of mixed lanthanide(III) and iron(III) oxo-phosphates is isolated. Black-Right-Pointing-Pointer Features PO{sub 4}-linked paramagnetic units of LnO{sub 6}, FeO{sub 5}, and {mu}{sub 3}-oxo [Fe{sub 4}O{sub 18}]. Black-Right-Pointing-Pointer Sm-derivative orders at T{sub N}{approx}7 K while Gd- and Dy-derivatives show absence of order.

Sanjeewa, Liurukara D.; Palmer West, J. [Department of Chemistry, Clemson University, Clemson, SC 29634-0973 (United States); Hwu, Shiou-Jyh, E-mail: shwu@clemson.edu [Department of Chemistry, Clemson University, Clemson, SC 29634-0973 (United States)

2012-08-15T23:59:59.000Z

453

The X-ray Crystallographic Structure and Activity Analysis of a Pseudomonas-Specific Subfamily of the HAD Enzyme Superfamily Evidences a Novel Biochemical Function  

Science Conference Proceedings (OSTI)

The haloacid dehalogenase (HAD) superfamily is a large family of proteins dominated by phosphotransferases. Thirty-three sequence families within the HAD superfamily (HADSF) have been identified to assist in function assignment. One such family includes the enzyme phosphoacetaldehyde hydrolase (phosphonatase). Phosphonatase possesses the conserved Rossmanniod core domain and a C1-type cap domain. Other members of this family do not possess a cap domain and because the cap domain of phosphonatase plays an important role in active site desolvation and catalysis, the function of the capless family members must be unique. A representative of the capless subfamily, PSPTO{_}2114, from the plant pathogen Pseudomonas syringae, was targeted for catalytic activity and structure analyses. The X-ray structure of PSPTO{_}2114 reveals a capless homodimer that conserves some but not all of the intersubunit contacts contributed by the core domains of the phosphonatase homodimer. The region of the PSPTO{_}2114 that corresponds to the catalytic scaffold of phosphonatase (and other HAD phosphotransfereases) positions amino acid residues that are ill suited for Mg+2 cofactor binding and mediation of phosphoryl group transfer between donor and acceptor substrates. The absence of phosphotransferase activity in PSPTO{_}2114 was confirmed by kinetic assays. To explore PSPTO{_}2114 function, the conservation of sequence motifs extending outside of the HADSF catalytic scaffold was examined. The stringently conserved residues among PSPTO{_}2114 homologs were mapped onto the PSPTO{_}2114 three-dimensional structure to identify a surface region unique to the family members that do not possess a cap domain. The hypothesis that this region is used in protein-protein recognition is explored to define, for the first time, HADSF proteins which have acquired a function other than that of a catalyst. Proteins 2008.

Peisach,E.; Wang, L.; Burroughs, A.; Aravind, L.; Dunaway-Mariano, D.; Allen, K.

2008-01-01T23:59:59.000Z

454

Novel Insights into the Diversity of Catabolic Metabolism from Ten Haloarchaeal Genomes  

E-Print Network (OSTI)

hydrolysis and biofuel production. Ind Eng Chem 89. Dadi AP,hydrolases for use in biofuel production are more likely todegradation related to biofuel production. An unexpected

Anderson, Iain

2012-01-01T23:59:59.000Z

455

Complete genome sequence of the thermophilic, hydrogen-oxidizing Bacillus tusciae type strain (T2T) and reclassification in the new genus, Kyrpidia gen. nov. as Kyrpidia tusciae comb. nov. and emendation of the family Alicyclobacillaceae da Costa and Rainey, 2010  

Science Conference Proceedings (OSTI)

Bacillus tusciae Bonjour & Aragno 1994 is a hydrogen-oxidizing, thermoacidophilic spore former that lives as a facultative chemolithoautotroph in solfataras. Although 16S rRNA gene sequencing was well established at the time of the initial description of the organism, 16S se- quence data were not available and the strain was placed into the genus Bacillus based on limited chemotaxonomic information. Despite the now obvious misplacement of strain T2T as a member of the genus Bacillus in 16S rRNA-based phylogenetic trees, the misclassification remained uncorrected for many years, which was likely due to the extremely difficult, analy- sis-hampering cultivation conditions and poor growth rate of the strain. Here we provide a taxonomic re-evaluation of strain T2T (= DSM 2912 = NBRC 15312) and propose its reclassi- fication as the type strain of a new species, Kyrpidia tusciae, and the type species of the new genus Kyrpidia, which is a sister-group of Alicyclobacillus. The family Alicyclobacillaceae da Costa and Rainey, 2010 is emended. The 3,384,766 bp genome with its 3,323 protein-coding and 78 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Copeland, A [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Chen, Feng [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Daum, Chris [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Chang, Yun-Juan [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Abt, Birte [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Pukall, Rudiger [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute

2011-01-01T23:59:59.000Z

456

Delivering Energy Efficiency to Middle Income Single Family Households  

E-Print Network (OSTI)

Home Energy Management DIY Do-It-Yourself HERS Homeare completed. Do-It-Yourself (DIY) Improvements. About oneand financial incentives for DIY improvements. Flexible

Zimring, Mark

2012-01-01T23:59:59.000Z

457

Delivering Energy Efficiency to Middle Income Single Family Households  

NLE Websites -- All DOE Office Websites (Extended Search)

AHPwES - Assisted Home Performance with ENERGY STAR AMI - Area Median Income APS - Arizona Public Service ARRA - American Reinvestment and Recovery Act ASEC - Annual Social and...

458

Second moment method for a family of boolean CSP  

E-Print Network (OSTI)

The estimation of phase transitions in random boolean Constraint Satisfaction Problems (CSP) is based on two fundamental tools: the first and second moment methods. While the first moment method on the number of solutions permits to compute upper bounds on any boolean CSP, the second moment method used for computing lower bounds proves to be more tricky and in most cases gives only the trivial lower bound 0. In this paper, we define a subclass of boolean CSP covering the monotone versions of many known NP-Complete boolean CSPs. We give a method for computing non trivial lower bounds for any member of this subclass. This is achieved thanks to an application of the second moment method to some selected solutions called characteristic solutions that depend on the boolean CSP considered. We apply, as an example, this method to establish that the threshold r_{k} of monotone 1-in-k-SAT is \\log k/k\\leq r_{k}\\leq\\log^{2}k/k

Boufkhad, Yacine

2011-01-01T23:59:59.000Z

459

Fish Bulletin No. 118. California Abalones, Family Haliotidae  

E-Print Network (OSTI)

collected at Unalaska, Aleutians, Dall (1873), states, "as an inhabitant of the Aleutian chain, does not exist innever found Haliotis in the Aleutian Islands." Abalones are

Cox, Keith W

1962-01-01T23:59:59.000Z