National Library of Energy BETA

Sample records for gene transfection view

  1. Hydrophobic Moiety of Cationic Lipids Strongly Modulates Their Transfection

    Office of Scientific and Technical Information (OSTI)

    Activity (Journal Article) | SciTech Connect Hydrophobic Moiety of Cationic Lipids Strongly Modulates Their Transfection Activity Citation Details In-Document Search Title: Hydrophobic Moiety of Cationic Lipids Strongly Modulates Their Transfection Activity Synthetic cationic lipids are widely used components of nonviral gene carriers, and the factors regulating their transfection efficiency are the subject of considerable interest. In view of the important role that electrostatic

  2. Cationic phospholipids: structure transfection activity relationships

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Cationic phospholipids: structure transfection activity relationships Citation Details In-Document Search Title: Cationic phospholipids: structure transfection activity relationships Synthetic cationic lipids are presently the most widely used non-viral gene carriers. Examined here is a particularly attractive cationic lipid class, triester phosphatidylcholines (PCs) exhibiting low toxicities and good transfection efficiency. Similarly to other cationic

  3. Cationic phospholipids: structure transfection activity relationships

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Cationic phospholipids: structure transfection activity relationships Citation Details In-Document Search Title: Cationic phospholipids: structure transfection activity relationships Synthetic cationic lipids are presently the most widely used non-viral gene carriers. Examined here is a particularly attractive cationic lipid class, triester phosphatidylcholines (PCs) exhibiting low toxicities and good transfection efficiency. Similarly to other cationic

  4. Cationic phospholipids: structure transfection activity relationships...

    Office of Scientific and Technical Information (OSTI)

    Cationic phospholipids: structure transfection activity relationships Citation Details In-Document Search Title: Cationic phospholipids: structure transfection activity ...

  5. Reduced expression of Autographa californica nucleopolyhedrovirus ORF34, an essential gene, enhances heterologous gene expression

    SciTech Connect (OSTI)

    Salem, Tamer Z.; Department of Microbial Molecular Biology, AGERI, Agricultural Research Center, Giza 12619; Division of Biomedical Sciences, Zewail University, Zewail City of Science and Technology, Giza 12588 ; Zhang, Fengrui; Thiem, Suzanne M.

    2013-01-20

    Autographa californica multiple nucleopolyhedrovirus ORF34 is part of a transcriptional unit that includes ORF32, encoding a viral fibroblast growth factor (FGF) and ORF33. We identified ORF34 as a candidate for deletion to improve protein expression in the baculovirus expression system based on enhanced reporter gene expression in an RNAi screen of virus genes. However, ORF34 was shown to be an essential gene. To explore ORF34 function, deletion (KO34) and rescue bacmids were constructed and characterized. Infection did not spread from primary KO34 transfected cells and supernatants from KO34 transfected cells could not infect fresh Sf21 cells whereas the supernatant from the rescue bacmids transfection could recover the infection. In addition, budded viruses were not observed in KO34 transfected cells by electron microscopy, nor were viral proteins detected from the transfection supernatants by western blots. These demonstrate that ORF34 is an essential gene with a possible role in infectious virus production.

  6. JOBAID-VIEWING USER RECORDS

    Office of Energy Efficiency and Renewable Energy (EERE)

    In this job aid you will View To-Do List using Filter and View options, View Completed Work, and View Curriculum Status and Detials areas.

  7. ThreatView

    Energy Science and Technology Software Center (OSTI)

    2007-09-25

    The ThreatView project is based on our prior work with the existing ParaView open-source scientific visualization application. Where ParaView provides a grapical client optimized scientific visualization over the VTK parallel client server architecture, ThreatView provides a client optimized for more generic visual analytics over the same architecture. Because ThreatView is based on the VTK parallel client-server architecture, data sources can reside on remote hosts, and processing and rendering can be performed in parallel. As seenmore » in Fig. 1, ThreatView provides four main methods for visualizing data: Landscape View, which displays a graph using a landscape metaphor where clusters of graph nodes produce "hills" in the landscape; Graph View, which displays a graph using a traditional "ball-and-stick" style; Table View, which displays tabular data in a standard spreadsheet; and Attribute View, which displays a tabular "histogram" of input data - for a selected table column, the Attribute View displays each unique value within the column, and the number of times that value appears in the data. There are two supplemental view types: Text View, which displays tabular data one-record-at-a-time; and the Statistics View, which displays input metadata, such as the number of vertices and edges in a graph, the number of rows in a table, etc.« less

  8. Self-assembled pentablock copolymers for selective and sustained gene delivery

    SciTech Connect (OSTI)

    Zhang, Bingqi

    2011-05-15

    The poly(diethylaminoethyl methacrylate) (PDEAEM) - Pluronic F127 - PDEAEM pentablock copolymer (PB) gene delivery vector system has been found to possess an inherent selectivity in transfecting cancer cells over non-cancer cells in vitro, without attaching any targeting ligands. In order to understand the mechanism of this selective transfection, three possible intracellular barriers to transfection were investigated in both cancer and non-cancer cells. We concluded that escape from the endocytic pathway served as the primary intracellular barrier for PB-mediated transfection. Most likely, PB vectors were entrapped and rendered non-functional in acidic lysosomes of non-cancer cells, but survived in less acidic lysosomes of cancer cells. The work highlights the importance of identifying intracellular barriers for different gene delivery systems and provides a new paradigm for designing targeting vectors based on intracellular differences between cell types, rather than through the use of targeting ligands. The PB vector was further developed to simultaneously deliver anticancer drugs and genes, which showed a synergistic effect demonstrated by significantly enhanced gene expression in vitro. Due to the thermosensitive gelation behavior, the PB vector packaging both drug and gene was also investigated for its in vitro sustained release properties by using polyethylene glycol diacrylate as a barrier gel to mimic the tumor matrix in vivo. Overall, this work resulted in the development of a gene delivery vector for sustained and selective gene delivery to tumor cells for cancer therapy.

  9. Isolation and characterization of Agouti: a diabetes/obesity related gene

    DOE Patents [OSTI]

    Woychik, Richard P.

    2000-06-27

    The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

  10. Isolation and characterization of Agouti: a diabetes/obesity related gene

    DOE Patents [OSTI]

    Woychik, Richard P.

    1998-01-01

    The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

  11. Taking the long view

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2012 Los Alamos Aerial Aerial view of a canyon in Los Alamos, New Mexico. Contact Environmental Communication & Public Outreach P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505)...

  12. Stereoscopic optical viewing system

    DOE Patents [OSTI]

    Tallman, Clifford S.

    1987-01-01

    An improved optical system which provides the operator a stereoscopic viewing field and depth of vision, particularly suitable for use in various machines such as electron or laser beam welding and drilling machines. The system features two separate but independently controlled optical viewing assemblies from the eyepiece to a spot directly above the working surface. Each optical assembly comprises a combination of eye pieces, turning prisms, telephoto lenses for providing magnification, achromatic imaging relay lenses and final stage pentagonal turning prisms. Adjustment for variations in distance from the turning prisms to the workpiece, necessitated by varying part sizes and configurations and by the operator's visual accuity, is provided separately for each optical assembly by means of separate manual controls at the operator console or within easy reach of the operator.

  13. REACTOR VIEWING APPARATUS

    DOE Patents [OSTI]

    Monk, G.S.

    1959-01-13

    An optical system is presented that is suitable for viewing objects in a region of relatively high radioactivity, or high neutron activity, such as a neutronic reactor. This optical system will absorb neutrons and gamma rays thereby protecting personnel fronm the harmful biological effects of such penetrating radiations. The optical system is comprised of a viewing tube having a lens at one end, a transparent solid member at the other end and a transparent aqueous liquid completely filling the tube between the ends. The lens is made of a polymerized organic material and the transparent solid member is made of a radiation absorbent material. A shield surrounds the tube betwcen the flanges and is made of a gamma ray absorbing material.

  14. Stereoscopic optical viewing system

    DOE Patents [OSTI]

    Tallman, C.S.

    1986-05-02

    An improved optical system which provides the operator with a stereoscopic viewing field and depth of vision, particularly suitable for use in various machines such as electron or laser beam welding and drilling machines. The system features two separate but independently controlled optical viewing assemblies from the eyepiece to a spot directly above the working surface. Each optical assembly comprises a combination of eye pieces, turning prisms, telephoto lenses for providing magnification, achromatic imaging relay lenses and final stage pentagonal turning prisms. Adjustment for variations in distance from the turning prisms to the workpiece, necessitated by varying part sizes and configurations and by the operator's visual accuity, is provided separately for each optical assembly by means of separate manual controls at the operator console or within easy reach of the operator.

  15. The View from HQ

    National Nuclear Security Administration (NNSA)

     NA-ASC-500-07 Issue 2 January 2007 The View from HQ Sitting in airports and planes is risky beyond the obvious dangers now in the news. Uninter- rupted time to think may lead to new ideas. Instinct instructs us that when we hear Wash- ington has some new ideas, the result must be bad. After all, ideas suggest change, which is inherently disruptive. Today the notion of predictivity is on my mind as I am leaving the V&V 2007 meeting in Los Alamos. Predictivity is on my short list of

  16. The View from HQ

    National Nuclear Security Administration (NNSA)

    A publication of the Office of Advanced Simulation & Computing, NNSA Defense Programs NA-ASC-500-07-Issue 3 May 2007 The View from HQ by Dimitri Kusnezov I have been spending much of my time these days thinking about science, technology and engineering and the role of the laboratories and how that will be reflected in the Complex of the future. This is on my mind for two reasons: one is my responsibility to produce a science and technology roadmap for Complex 2030-Defense Program's vision

  17. Recent developments with the human repair genes ERCC2, ERCC4, and XRCC1

    SciTech Connect (OSTI)

    Thompson, L.H.; Caldecott, K.W.; Brookman, K.W.; Weber, C.A.; Salazar, E.S.; Takayama, K.; Fornace, A.J.

    1992-11-06

    ERCC2 was first identified as a gene on human chromosome 19 that complemented the UV sensitivity of CHO UV5 cells in somatic cell hybrids. Subsequent studies localized ERCC2 to the same chromosomal region (19q13.2--13.3) as the ERCC1 gene and showed that the two genes were less than 250 kb apart. Cloning of ERCC2 was accomplished by transfection of genomic DNA into UV5 cells and rescue of the gene from a secondary transformant. Recovery of the gene was aided by the presence of repetitive sequences that were detected on Southern blots with a probe for Alu-family repeats. ERCC2, which is 19 kb in size, quantitatively corrected the UV sensitivity and incision defect in UV5 cells upon transfection. An ERCC2 CDNA clone was recovered from the pcD2 expression library. Although this clone was truncated at the 5 in. end, it conferred transient, but not stable, correction to UV5 cells upon transfection. Based on genomic sequence, this clone was extended by oligonucleotide addition to obtain minigene constructs in which the complete open reading frame (ORF) was present. Translation of the ERCC2 ORF gives an amino acid sequence that has 72% similarity with the S. cerevisiae RAD3 protein, which encodes a DNA helicase.

  18. Overexpression of the human BCL-2 gene product results in growth enhancement of Epstein-Barr virus-immortalized B cells

    SciTech Connect (OSTI)

    Tsujimoto, Yoshihide (Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA))

    1989-03-01

    The biological activity of the human BCL-2 gene product was analyzed in an Epstein-Barr virus (EBV)-infected human lymphoblastoid B-cell line transfected with BCL-2 sequences driven by the simian virus 40 promoter and enhancer. Overproduction of the BCL-2 protein conferred a selective growth advantage to the EBV-infected B cells as compared with control transfectants in low-serum medium and also after seeding at limiting dilution but did not render the cells tumorigenic in athymic nude mice. This growth enhancement was also seen in cells transfected with the BCL-2 gene with its own promoter juxtaposed to the immunoglobulin heavy chain gene enhancer, which represents the translocated form of the BCL-2 gene observed in follicular lymphomas with the t(14;18) translocation. The growth advantage of EBV-infected B cells overproducing the BCL-2 protein is neither due to the enhanced growth factor production nor due to an enhanced sensitivity of the BCL-2 transfectants to interleukins 1 or 6, although both lymphokines are known to stimulate proliferation of EBV-infected B-cell lines. The growth advantage of EBV-infected B-cell lines. The growth advantage of EBV-infected B cells by overproduction of the BCL-2 protein suggests the direct involvement of the BCL-2 gene product in the pathogenesis of follicular lymphoma.

  19. Protective laser beam viewing device

    DOE Patents [OSTI]

    Neil, George R.; Jordan, Kevin Carl

    2012-12-18

    A protective laser beam viewing system or device including a camera selectively sensitive to laser light wavelengths and a viewing screen receiving images from the laser sensitive camera. According to a preferred embodiment of the invention, the camera is worn on the head of the user or incorporated into a goggle-type viewing display so that it is always aimed at the area of viewing interest to the user and the viewing screen is incorporated into a video display worn as goggles over the eyes of the user.

  20. False color viewing device

    DOE Patents [OSTI]

    Kronberg, J.W.

    1991-05-08

    This invention consists of a viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching, the user`s eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage.

  1. False color viewing device

    DOE Patents [OSTI]

    Kronberg, James W.

    1992-01-01

    A viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching the user's eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage.

  2. False color viewing device

    DOE Patents [OSTI]

    Kronberg, J.W.

    1992-10-20

    A viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching the user's eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage. 7 figs.

  3. Mountain View Grand | Open Energy Information

    Open Energy Info (EERE)

    Mountain View Grand Jump to: navigation, search Name Mountain View Grand Facility Mountain View Grand Sector Wind energy Facility Type Small Scale Wind Facility Status In Service...

  4. Fermilab | Tritium at Fermilab | Ferry Creek Aerial View

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ferry Creek Aerial View Ferry Creek Aerial View

  5. Fermilab | Tritium at Fermilab | Kress Creek Aerial View

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kress Creek Aerial View Kress Creek Aerial View

  6. Highland View school | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Highland View school Highland View school Aerial showing Highland View school and surrounding homes

  7. Radiation View Factor With Shadowing

    Energy Science and Technology Software Center (OSTI)

    1992-02-24

    FACET calculates the radiation geometric view factor (alternatively called shape factor, angle factor, or configuration factor) between surfaces for axisymmetric, two-dimensional planar and three-dimensional geometries with interposed third surface obstructions. FACET was developed to calculate view factors as input data to finite element heat transfer analysis codes.

  8. Dendritic cells transduced with Rsf-1/HBXAP gene generate specific cytotoxic T lymphocytes against ovarian cancer in vitro

    SciTech Connect (OSTI)

    Sun, Li; Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong ; Kong, Beihua; Sheng, Xiugui; Sheu, Jim Jinn-Chyuan; Shih, Ie-Ming

    2010-04-09

    Recently, some studies have indicated that Rsf-1/HBXAP plays a role in chromatin remodeling and transcriptional regulation that may contribute to tumorigenesis in ovarian cancer. The present study demonstrates that using dendritic cells (DCs) from human cord blood CD34{sup +} cells transduced with Rsf-1/HBXAP DNA plasmids by nucleofection generate specific cytotoxic T lymphocytes (CTL) against ovarian cancer in vitro. After transfection, DCs were analyzed for Rsf-1/HBXAP mRNA expression by RT-PCR and protein expression by Western blot. Then the DC phenotypes, T-cell stimulatory capacity, endocytic activity and migration capacity were explored by flow cytometry analysis, allogeneic mixed lymphocyte reaction, endocytosis and transwell chemotaxis assay, respectively. After transfection, Rsf-1/HBXAP expression was detected at mRNA and protein levels. Allogeneic T-cell proliferation induced by transfected DCs was obviously higher than non-transfected DCs, but the endocytosis capacity and migratory ability were not different. Rsf-1/HBXAP gene-transduced DCs could induce antigen-specific CTL and generate a very potent cytotoxicity to OVCAR3 cells. These data suggest that Rsf-1/HBXAP gene-transduced DCs may be a potential adjuvant immunotherapy for ovarian cancer in clinical applications.

  9. TotalView Training 2015

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TotalView Training 2015 TotalView Training 2015 NERSC will host an in-depth training course on TotalView, a graphical parallel debugger developed by Rogue Wave Software, on Thursday, March 26, 2015. This will be provided by Rogue Wave Software staff members. The training will include a lecture and demo sessions in the morning, followed by a hands-on parallel debugging session in the afternoon. Location This event will be presented online using WebEx technology and in person at NERSC Oakland

  10. September 2013 Most Viewed Documents for Renewable Energy Sources | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Renewable Energy Sources Science Subject Feed Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 362 /> Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 103 /> Chapter 17. Engineering cost analysis Higbee, Charles V. (1998) 79 /> Advanced Electric Submersible Pump Design Tool for Geothermal Applications Xuele Qi; Norman Turnquist;

  11. Most Viewed Documents for Renewable Energy Sources: December 2014 | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Renewable Energy Sources: December 2014 Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 339 Generalized displacement correlation method for estimating stress intensity factors Fu, P; Johnson, S M; Settgast, R R; Carrigan, C R (2011) 107 Seventh Edition Fuel Cell Handbook NETL (2004) 96 Advanced Electric Submersible Pump Design Tool for Geothermal Applications Xuele Qi; Norman

  12. Trichoderma genes

    DOE Patents [OSTI]

    Foreman, Pamela; Goedegebuur, Frits; Van Solingen, Pieter; Ward, Michael

    2012-06-19

    Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.

  13. Wide field of view telescope

    DOE Patents [OSTI]

    Ackermann, Mark R.; McGraw, John T.; Zimmer, Peter C.

    2008-01-15

    A wide field of view telescope having two concave and two convex reflective surfaces, each with an aspheric surface contour, has a flat focal plane array. Each of the primary, secondary, tertiary, and quaternary reflective surfaces are rotationally symmetric about the optical axis. The combination of the reflective surfaces results in a wide field of view in the range of approximately 3.8.degree. to approximately 6.5.degree.. The length of the telescope along the optical axis is approximately equal to or less than the diameter of the largest of the reflective surfaces.

  14. Views of the solar system

    SciTech Connect (OSTI)

    Hamilton, C.

    1995-02-01

    Views of the Solar System has been created as an educational tour of the solar system. It contains images and information about the Sun, planets, moons, asteroids and comets found within the solar system. The image processing for many of the images was done by the author. This tour uses hypertext to allow space travel by simply clicking on a desired planet. This causes information and images about the planet to appear on screen. While on a planet page, hyperlinks travel to pages about the moons and other relevant available resources. Unusual terms are linked to and defined in the Glossary page. Statistical information of the planets and satellites can be browsed through lists sorted by name, radius and distance. History of Space Exploration contains information about rocket history, early astronauts, space missions, spacecraft and detailed chronology tables of space exploration. The Table of Contents page has links to all of the various pages within Views Of the Solar System.

  15. For current viewing resistor loads

    DOE Patents [OSTI]

    Lyons, Gregory R.; Hass, Jay B.

    2011-04-19

    The invention comprises a terminal unit for a flat cable comprising a BNC-PCB connector having a pin for electrically contacting one or more conducting elements of a flat cable, and a current viewing resistor having an opening through which the pin extends and having a resistor face that abuts a connector face of the BNC-PCB connector, wherein the device is a terminal unit for the flat cable.

  16. Better Buildings Network View | March 2014

    Broader source: Energy.gov [DOE]

    The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network.

  17. Better Buildings Network View | January 2015

    Broader source: Energy.gov [DOE]

    The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network.

  18. Better Buildings Network View | June 2015

    Broader source: Energy.gov [DOE]

    The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network.

  19. Better Buildings Network View | May 2014

    Broader source: Energy.gov [DOE]

    The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network.

  20. The Better Buildings Neighborhood View-- July 2012

    Broader source: Energy.gov [DOE]

    The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program.

  1. The Better Buildings Neighborhood View-- Fall 2011

    Broader source: Energy.gov [DOE]

    Better Buildings Neighborhood View, from the Better Buildings Neighborhood Program of the U.S. Department of Energy.

  2. June 2014 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Renewable Energy Sources Science Subject Feed Chapter 6. Drilling and Well Construction Culver, Gene (1998) 426 /> Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 300 /> Seventh Edition Fuel Cell Handbook NETL (2004) 118 /> Chapter 17. Engineering cost analysis Higbee, Charles V. (1998) 115 /> Generalized displacement correlation method for estimating stress

  3. March 2014 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Renewable Energy Sources Science Subject Feed Chapter 6. Drilling and Well Construction Culver, Gene (1998) 299 /> Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 184 /> Chapter 17. Engineering cost analysis Higbee, Charles V. (1998) 124 /> Solar radiation data manual for flat-plate and concentrating collectors Dunlap, M.A. [ed.]; Marion, W.; Wilcox, S. (null) 74 />

  4. View Shed - Version 1.0

    Energy Science and Technology Software Center (OSTI)

    2014-09-18

    The View Shed library is a collection of Umbra modules that are used to calculate areas of visual coverage (view sheds). It maps high and low visibility areas and calculates sensor (camera placement for maximum coverage and performance. This assertion includes a managed C++ wrapper code (ViewShedWrapper) to enable C# applications, such as OpShed, to incorporate this library.

  5. Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity

    SciTech Connect (OSTI)

    Yan, Chen; Jie, Leng; Yongqi, Wang; Weiming, Xiao; Juqun, Xi; Yanbing, Ding; Li, Qian; Xingyuan, Pan; Mingchun, Ji; Weijuan, Gong

    2015-07-31

    Nanoparticles are becoming promising carriers for gene delivery because of their high capacity in gene loading and low cell cytotoxicity. In this study, a chitosan-based nanoparticle encapsulated within a recombinant pcDNA3.1-dsNKG2D-IL-15 plasmid was generated. The fused dsNKG2D-IL-15 gene fragment consisted of double extracellular domains of NKG2D with IL-15 gene at downstream. The average diameter of the gene nanoparticles ranged from 200 nm to 400 nm, with mean zeta potential value of 53.8 ± 6.56 mV. The nanoparticles which were loaded with the dsNKG2D-IL-15 gene were uptaken by tumor cells with low cytotoxicity. Tumor cells pre-transfected by gene nanopartilces stimulated NK and T cells in vitro. Intramuscular injection of gene nanoparticles suppressed tumor growth and prolonged survival of tumor-bearing mice through activation of NK and CD8{sup +} T cells. Thus, chitosan-based nanoparticle delivery of dsNKG2D-IL-15 gene vaccine can be potentially used for tumor therapy. - Highlights: • Generation of a nanoparticle for delivery of dsNKG2D-IL-15 gene. • Characterization of the gene nanoparticle. • Antitumor activity mediated by the gene nanoparticle.

  6. The BetterBuildings View | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The BetterBuildings View The BetterBuildings View The BetterBuildings View Newsletter, April 2011, from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon The BetterBuildings View April 2011 More Documents & Publications The Better Buildings Neighborhood View - October 2012 The Better Buildings Neighborhood View -- Fall 2011 The Better Buildings Neighborhood View -- April 2013

  7. Prairie View Gas Recovery Biomass Facility | Open Energy Information

    Open Energy Info (EERE)

    View Gas Recovery Biomass Facility Jump to: navigation, search Name Prairie View Gas Recovery Biomass Facility Facility Prairie View Gas Recovery Sector Biomass Facility Type...

  8. Better Buildings Network View | September 2015 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Better Buildings Network View | September 2015 Better Buildings Network View | September 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of...

  9. Better Buildings Network View, April 2015

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... systems Better Buildings Network View financial sector. ... Sunnovations, Inc., is a McLean, Virginia-based firm offering ... as featured in the new Resource Corner Designing ...

  10. High Performance Builder Spotlight: Clifton View Homes

    SciTech Connect (OSTI)

    2011-01-01

    Clifton View Homess remodel of a 1962 rambler, on Whidbey Island in Washington State, cut energy costs by two-thirds.

  11. Better Buildings Network View, May 2015

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... now Leveraging Seasonal Opportunities for Marketing Better Buildings Network View making. ... Prize (GUEP) participants are going digital to encourage people across the United ...

  12. The Better Buildings Neighborhood View-- July 2013

    Broader source: Energy.gov [DOE]

    The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program - July 2013.

  13. Employee Spotlight: Gene Ortega

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gene Ortega Gene Ortega-The eyes have it Painting with a style that mixes chaos and refinement, Gene Ortega has found a way to blend his technical and artistic sides. May 4, 2016 gene ortega gene ortega gene ortega gene ortega "I focus on the eyes because they give the painting a soul." The eyes have it gene ortega Gene Ortega paints portraits rich in color and symbolism. "My style is chaotic with a bit of refinement," says Gene, a systems engineer in Facility System

  14. The Better Buildings Neighborhood View -- April 2012 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 The Better Buildings Neighborhood View -- April 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- April 2012 More Documents & Publications The Better Buildings Neighborhood View -- March 2012 The Better Buildings Neighborhood View - September 2012 The Better Buildings Neighborhood View -- July 2012

  15. The Better Buildings Neighborhood View -- April 2013 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 The Better Buildings Neighborhood View -- April 2013 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program - April 2013. PDF icon BB Neighborhood View -- April 2013 More Documents & Publications The Better Buildings Neighborhood View -- July 2013 The Better Buildings Neighborhood View -- January 2013 The Better Buildings Neighborhood View -- December 2013

  16. The Better Buildings Neighborhood View -- August 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy August 2012 The Better Buildings Neighborhood View -- August 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- August 2012 More Documents & Publications The Better Buildings Neighborhood View - September 2012 The Better Buildings Neighborhood View -- July 2012 The Better Buildings Neighborhood View -- June 2012

  17. The Better Buildings Neighborhood View -- January 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy 2 The Better Buildings Neighborhood View -- January 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- January 2012 More Documents & Publications The Better Buildings Neighborhood View -- March 2012 The Better Buildings Neighborhood View -- February 2012 The BetterBuildings View

  18. The Better Buildings Neighborhood View -- June 2012 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    June 2012 The Better Buildings Neighborhood View -- June 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- June 2012 More Documents & Publications The Better Buildings Neighborhood View -- August 2012 The Better Buildings Neighborhood View - October 2012 The Better Buildings Neighborhood View -- April 2012

  19. Better Buildings Network View | April 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | April 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View April 2015 More Documents & Publications Better Buildings Network View | May 2015 Better Buildings Network View | March 2015 Better Buildings Network View | July-August

  20. Better Buildings Network View | April 2016 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Better Buildings Network View | April 2016 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View April 2016 More Documents & Publications Better Buildings Network View | March 2016 Better Buildings Network View | January 2016 Better Buildings Network View | February 2016

  1. Better Buildings Network View | February 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | February 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View February 2014 More Documents & Publications Better Buildings Network View | January 2014 Better Buildings Network View | May 2015 Better Buildings Network View | June 2015

  2. Better Buildings Network View | February 2016 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Better Buildings Network View | February 2016 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View February 2016 More Documents & Publications Better Buildings Network View | March 2016 Better Buildings Network View | June 2014 Better Buildings Network View | April 2016

  3. Better Buildings Network View | January 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | January 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View January 2014 More Documents & Publications Better Buildings Network View | February 2015 Better Buildings Network View | May 2015 Better Buildings Network View | September 2014

  4. Better Buildings Network View | January 2016 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Better Buildings Network View | January 2016 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View January 2016 More Documents & Publications Better Buildings Network View | October 2015 Better Buildings Network View | April 2016 Better Buildings Network View | December

  5. Better Buildings Network View | June 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | June 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View June 2014 More Documents & Publications Better Buildings Network View | June 2015 Better Buildings Network View | July-August 2014 Better Buildings Network View | April 2014

  6. Better Buildings Network View | March 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | March 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View March 2015 More Documents & Publications Better Buildings Network View | January 2015 Better Buildings Network View | December 2014 Better Buildings Network View | April 2015

  7. Better Buildings Network View | March 2016 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Better Buildings Network View | March 2016 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View March 2016 More Documents & Publications Better Buildings Network View | April 2016 Better Buildings Network View | February 2016 Better Buildings Network View | January 2016

  8. Better Buildings Network View | October 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | October 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View October 2015 More Documents & Publications Better Buildings Network View | January 2016 Better Buildings Network View | April 2016 Better Buildings Network View | November

  9. The Better Buildings Neighborhood View - October 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy The Better Buildings Neighborhood View - October 2012 The Better Buildings Neighborhood View - October 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program - October 2012 PDF icon bb_view_october2012.pdf More Documents & Publications The Better Buildings Neighborhood View -- July 2013 The Better Buildings Neighborhood View -- Fall 2011 The Better Buildings Neighborhood View -- December 2013

  10. A common-view disciplined oscillator

    SciTech Connect (OSTI)

    Lombardi, Michael A.; Dahlen, Aaron P.

    2010-05-15

    This paper describes a common-view disciplined oscillator (CVDO) that locks to a reference time scale through the use of common-view global positioning system (GPS) satellite measurements. The CVDO employs a proportional-integral-derivative controller that obtains near real-time common-view GPS measurements from the internet and provides steering corrections to a local oscillator. A CVDO can be locked to any time scale that makes real-time common-view data available and can serve as a high-accuracy, self-calibrating frequency and time standard. Measurement results are presented where a CVDO is locked to UTC(NIST), the coordinated universal time scale maintained at the National Institute of Standards and Technology in Boulder, Colorado.

  11. TotalView Parallel Debugger at NERSC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The performance of the GUI can be greatly improved if used in conjunction with free NX software. The TotalView documentation web page is a good resource for learning more...

  12. JOBAID-VIEWING AN EMPLOYEE MATRIX (SUPERVISOR)

    Broader source: Energy.gov [DOE]

    The purpose of this job aid is to guide supervisor users through the step-by-step process of viewing an employee matrix within SuccessFactors Learning.

  13. The Better Buildings Neighborhood View - Summer 2011 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Summer 2011 The Better Buildings Neighborhood View - Summer 2011 The quarterly update newsletter of the Better Buildings program of the U.S. Department of Energy. PDF icon BB Neighborhood View -- Summer 2011 More Documents & Publications The Better Buildings Neighborhood View -- January 2012 The Better Buildings Neighborhood View -- March 2012 The Better Buildings Neighborhood View -- May

  14. The Better Buildings Neighborhood View -- March 2012 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    March 2012 The Better Buildings Neighborhood View -- March 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program PDF icon BB Neighborhood View -- March 2012 More Documents & Publications The Better Buildings Neighborhood View -- February 2012 The Better Buildings Neighborhood View -- January 2012 Commercial Buildings Integration Program Overview - 2015 BTO Peer Review

  15. The Better Buildings Neighborhood View -- May 2012 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - May 2012 The Better Buildings Neighborhood View -- May 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program PDF icon BB Neighborhood View -- May 2012 More Documents & Publications The Better Buildings Neighborhood View -- June 2012 The Better Buildings Neighborhood View - September 2012

  16. Better Buildings Network View | April 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | April 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View April 2014 More Documents & Publications Better Buildings Network View | December 2014 Better Buildings Residential Network Orientation Webinar Better Buildings Network View | May

  17. Better Buildings Network View | December 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | December 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View December 2014 More Documents & Publications Better Buildings Network View | February 2014 Better Buildings Network View | November 2014 Lessons Learned: Peer Exchange Calls -- No. 3

  18. Better Buildings Network View | February 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | February 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View February 2015 More Documents & Publications Better Buildings Network View | June 2015 Nothing But Networking for Residential Network Members Better Buildings Network View | November 2014

  19. Better Buildings Network View | May 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | May 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View May 2015 More Documents & Publications Better Buildings Network View | June 2015 Home Performance with ENERGY STAR - 2014 BTO Peer Review Better Buildings Network View | April 2015

  20. Better Buildings Network View | November 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | November 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View November 2014 More Documents & Publications Better Buildings Network View | July-August 2014 Better Buildings Residential Network Orientation Webinar Better Buildings Network View | December 2014

  1. Better Buildings Network View | October 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    October 2014 Better Buildings Network View | October 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View October 2014 More Documents & Publications Better Buildings Network View | September 2014 Better Buildings Network View | December 2014

  2. Compare Gene Profiles

    Energy Science and Technology Software Center (OSTI)

    2014-05-31

    Compare Gene Profiles (CGP) performs pairwise gene content comparisons among a relatively large set of related bacterial genomes. CGP performs pairwise BLAST among gene calls from a set of input genome and associated annotation files, and combines the results to generate lists of common genes, unique genes, homologs, and genes from each genome that differ substantially in length from corresponding genes in the other genomes. CGP is implemented in Python and runs in a Linuxmore » environment in serial or parallel mode.« less

  3. April 2013 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Renewable Energy Sources Science Subject Feed Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 1252 /> Seventh Edition Fuel Cell Handbook NETL (2004) 628 /> Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 290 /> Chapter 17. Engineering cost analysis Higbee, Charles V. (1998) 223 /> Geothermal Power Generation - A Primer on

  4. July 2013 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Renewable Energy Sources Science Subject Feed Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 484 /> Environmental Impacts of Wind Power Development on the Population Biology of Greater Prairie-Chickens Sandercock, Brett K. [Kansas State University] (2013) 184 /> Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 154 /> A study of lead-acid

  5. March 2015 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Renewable Energy Sources Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 386 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 234 Aerodynamic characteristics of seven symmetrical airfoil sections through 180-degree angle of attack for use in aerodynamic analysis of vertical axis wind turbines Sheldahl, R E; Klimas, P C (1981) 159 Calculation of brine

  6. City of Mountain View, Missouri (Utility Company) | Open Energy...

    Open Energy Info (EERE)

    View Place: Missouri Phone Number: (417) 934-2601 Website: mountainviewmo.comindex.phpg Facebook: https:www.facebook.comCityOfMountainViewMissouri Outage Hotline: (877)...

  7. Mountain View Power Partners II Wind Farm | Open Energy Information

    Open Energy Info (EERE)

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  8. Mountain View Power Partners III Wind Farm | Open Energy Information

    Open Energy Info (EERE)

    III Wind Farm Jump to: navigation, search Name Mountain View Power Partners III Wind Farm Facility Mountain View Power Partners III Sector Wind energy Facility Type Commercial...

  9. OpenEI:Neutral point of view | Open Energy Information

    Open Energy Info (EERE)

    point of view Jump to: navigation, search Neutral point of view (NPOV) means that articles and content contributed or edited on the platform must be unbiased. All significant...

  10. The Better Buildings Neighborhood View -- January 2013 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  11. Better Buildings Network View | September 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  12. The Better Buildings Neighborhood View -- July 2013 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 The Better Buildings Neighborhood View -- July 2013 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood...

  13. The Better Buildings Neighborhood View -- April 2013 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 The Better Buildings Neighborhood View -- April 2013 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood...

  14. The Better Buildings Neighborhood View -- April 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 The Better Buildings Neighborhood View -- April 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood...

  15. The Better Buildings Neighborhood View -- August 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    August 2012 The Better Buildings Neighborhood View -- August 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings...

  16. Better Buildings Network View | November 2015 | Department of...

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  17. The Better Buildings Neighborhood View -- March 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    March 2012 The Better Buildings Neighborhood View -- March 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings...

  18. The Better Buildings Neighborhood View -- June 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  19. The Better Buildings Neighborhood View -- July 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  20. The Better Buildings Neighborhood View -- January 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 The Better Buildings Neighborhood View -- January 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings...

  1. The Better Buildings Neighborhood View -- May 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  2. The Better Buildings Neighborhood View -- Fall 2011 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fall 2011 The Better Buildings Neighborhood View -- Fall 2011 Better Buildings Neighborhood View, from the Better Buildings Neighborhood Program of the U.S. Department of Energy....

  3. Better Buildings Network View | April 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  4. Better Buildings Network View | January 2016 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  5. Better Buildings Network View | February 2015 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  6. Better Buildings Network View | December 2015 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  7. Better Buildings Network View | June 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | June 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon ...

  8. Better Buildings Network View | October 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  9. Better Buildings Network View | January 2015 | Department of...

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  10. Better Buildings Network View | May 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | May 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon ...

  11. Better Buildings Network View | February 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  12. Better Buildings Network View | November 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | November 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF ...

  13. Better Buildings Network View | July-August 2014 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | July-August 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. ...

  14. Better Buildings Network View | February 2016 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  15. Better Buildings Network View | May 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | May 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon ...

  16. Better Buildings Network View | March 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  17. Better Buildings Network View | April 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  18. Better Buildings Network View | December 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  19. Better Buildings Network View | March 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | March 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF ...

  20. Better Buildings Network View | January 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | January 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF ...

  1. Better Buildings Network View | October 2015 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | October 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF ...

  2. Better Buildings Network View | March 2016 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Better Buildings Network View | March 2016 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF ...

  3. Better Buildings Network View | June 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | June 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon ...

  4. Better Buildings Network View | July-August 2015 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | July-August 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. ...

  5. Residential Windows and Window Coverings: A Detailed View of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Residential Windows and Window Coverings: A Detailed View of the Installed Base and User Behavior Residential Windows and Window Coverings: A Detailed View of the Installed Base ...

  6. Our view: Vaccinate now, prevent flu later

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our view: Vaccinate now, prevent flu later Our view: Vaccinate now, prevent flu later Los Alamos National Laboratory scientists are predicting that this winter's flu season is most likely to peak in February across much of the United States. The scientists can say this because of the model they have constructed. December 24, 2015 Man sneezing Model suggests still time to get your flu shot and be protected. "There's no crystal ball when it comes to predicting disease outbreaks," said

  7. Linked-View Parallel Coordinate Plot Renderer

    Energy Science and Technology Software Center (OSTI)

    2011-06-28

    This software allows multiple linked views for interactive querying via map-based data selection, bar chart analytic overlays, and high dynamic range (HDR) line renderings. The major component of the visualization package is a parallel coordinate renderer with binning, curved layouts, shader-based rendering, and other techniques to allow interactive visualization of multidimensional data.

  8. The Better Buildings Neighborhood View - September 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy September 2012 The Better Buildings Neighborhood View - September 2012 Monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- September 2012 More Documents & Publications The Better Buildings Neighborhood View -- Fall 2011 The Better Buildings Neighborhood View -- July 2013

  9. Better Buildings Network View | December 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | December 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View December 2015 More Documents & Publications BBRN Factsheet: Case Study: Community Engagement Better Buildings Network View | July-August 2015 Better Buildings Residential Network Orientation Webinar

  10. Better Buildings Network View | September 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Network View | September 2015 Better Buildings Network View | September 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View September 2015 More Documents & Publications TTWG Licensing Guide ITP Aluminum: Technical Working Group on Inert Anode Technologies EIS-0333: Draft Environmental Impact Statement

  11. The Better Buildings Neighborhood View -- December 2013 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy December 2013 The Better Buildings Neighborhood View -- December 2013 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- December 2013 More Documents & Publications The Better Buildings Neighborhood View -- July 2013 Focus Series: Philadelphia Energyworks: In the City of Brotherly Love, Sharing Know-How Leads to Sustainability The Better Buildings Neighborhood View

  12. The Better Buildings Neighborhood View -- February 2012 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy February 2012 The Better Buildings Neighborhood View -- February 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB Neighborhood View -- February 2012 More Documents & Publications The Better Buildings Neighborhood View -- March 2012 The Better Buildings Neighborhood View -- January 2012 Microsoft Word - T4_VEIC_TO2_ Sub3_Residential Retrofit Program Design Guide Play Book_TEAM 4

  13. The Better Buildings Neighborhood View -- January 2013 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy 3 The Better Buildings Neighborhood View -- January 2013 The Better Buildings Neighborhood View monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program -- January 2013. PDF icon BB Neighborhood View -- January 2013 More Documents & Publications The Better Buildings Neighborhood View - September 2012 The Better Buildings Neighborhood View - October 2012 It's Academic: BetterBuildings for Michigan Partners With University to Reach Employees

  14. Employee Spotlight: Gene Ortega

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gene Ortega May 4, 2016 The eyes have it Gene Ortega paints portraits rich in color and symbolism. "My style is chaotic with a bit of refinement," says Gene, a systems engineer in Facility System Engineering. "It can be almost photo-realistic, but you can see the brushstrokes and the texture in it, and the chaos and the anger." Gene's vivid paintings explore religious iconography and Day of the Dead motifs in portraits, often drawing on the imagery of saints, the Virgin Mary,

  15. The Better Buildings Neighborhood View -- February 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    View -- March 2012 The Better Buildings Neighborhood View -- January 2012 Microsoft Word - T4VEICTO2 Sub3Residential Retrofit Program Design Guide Play BookTEAM 4 FINAL.docx

  16. ParaView Red Blood Cell Tutorial | Argonne Leadership Computing...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ParaView Red Blood Cell Tutorial Goals This tutorial is intended to be a hands-on resource ... Data set for ParaView Red Blood Cell Tutorial File Open Icon Load Multi-component Dataset ...

  17. ALS Technique Gives Novel View of Lithium Battery Dendrite Growth

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

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  18. New Window Technology Saves Energy and the View | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  19. Mountain View Power Partners I Wind Farm | Open Energy Information

    Open Energy Info (EERE)

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  20. Better Buildings Network View | September 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    September 2014 Better Buildings Network View | September 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View September 2014 More Documents & Publications Focus Series: OREGON-On Bill Financing Program: On-Bill Financing Brings Lenders and Homeowners On Board Better Buildings Network View | December 2014 On-Bill Financing for Energy E

  1. Better Buildings Network View | November 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    November 2015 Better Buildings Network View | November 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View November 2015 More Documents & Publications Lessons Learned: Peer Exchange Calls -- No. 3 Better Buildings Residential Network Membership Form

  2. Multidimensional display controller for displaying to a user an aspect of a multidimensional space visible from a base viewing location along a desired viewing orientation

    DOE Patents [OSTI]

    Davidson, George S.; Anderson, Thomas G.

    2001-01-01

    A display controller allows a user to control a base viewing location, a base viewing orientation, and a relative viewing orientation. The base viewing orientation and relative viewing orientation are combined to determine a desired viewing orientation. An aspect of a multidimensional space visible from the base viewing location along the desired viewing orientation is displayed to the user. The user can change the base viewing location, base viewing orientation, and relative viewing orientation by changing the location or other properties of input objects.

  3. Exaggerated supine oblique view of the cervical spine

    SciTech Connect (OSTI)

    Abel, M.S.

    1982-06-01

    The technique of the 60 degree supine oblique view is described together with anatomic skeletal studies of this projection. The view is convenient for emergency room radiography and useful in other clinical radiography. The view separates widely the anterior and posterior portions of the vertebrae in a side to side projection. This makes for an elongated but detailed view of the articular processes, pedicles, and intervertebral foramina. In the cadavar specimen and clinically the view is shown to be useful in delineating fracture deformities of the articular process and visualizing constriction of the intervertebral foramen superiorly. Encroachment of the foramen superiorly is likely to compromise the emerging nerve root in this area.

  4. Target Fabrication: A View from the Users

    SciTech Connect (OSTI)

    Kyrala, George A.; Balkey, Matthew M.; Barnes, Cris W.; Batha, Steven H.; Christensen, Cindy R.; Cobble, James A.; Fincke, James; Keiter, Paul; Lanier, Nicholas; Paisley, Dennis; Sorem, Michael; Swift, Damian; Workman, Jonathan

    2004-03-15

    Targets are used for a variety of purposes, but ultimately we use them to validate codes that help us predict and understand new phenomena or effects. The sophistication and complexity of High Energy Density Physics (HEDP) and Inertial Confinement Fusion (ICF) targets has increased in to match the advances made in modeling complex phenomena. The targets have changed from simple hohlraums, spherical geometries, and planar foils, to 3-dimensional geometries that require precision in construction, alignment, and metrology. Furthermore, material properties, such as surface morphologies and volume texture, have significant impact on the behavior of the targets and must be measured and controlled. In the following we will discuss how experimental physicists view targets and the influence that target construction has on interpreting the experimental results. We review a representative sampling of targets fabricated at the Los Alamos National Laboratory that are used in different experiments in support of ICF and HEDP.

  5. TARGET FABRICATION: A VIEW FROM THE USERS.

    SciTech Connect (OSTI)

    Kyrala, George A.; Balkey, Matthew M.; Batha, Steven H.; Barnes, Cris W.; Christensen, Cindy; Cobble, James; Fincke, James; Keiter, Paul; Lanier, Nicholas; Paisley, Dennis; Sorem, Michael S.; Swift, Damian; Workman, Jonathan

    2003-07-18

    Targets are used for a variety of purposes, but ultimately we use them to validate codes that help us predict and understand new phenomena or effects. The sophistication and complexity of High Energy Density Physics (HEDP) and Inertial Confinement Fusion (ICF) targets has increased in time to match the advances made in modeling complex phenomena. The targets have changed from simple hohlraums, spherical geometries, and planar foils, to 3-dimensional geometries that require precision in construction, alignment, and metrology. Furthermore, material properties, such as surface morphologies and volume texture, have significant impact on the behavior of the targets and must be measured and controlled. In the following we will discuss how experimental physicists view targets and the influence that target construction has on interpreting the experimental results. We review a representative sampling of targets fabricated at the Los Alamos National Laboratory that are used in different experiments in support of ICF and HEDP.

  6. The candidate tumor suppressor CST6 alters the gene expression profile of human breast carcinoma cells: Down-regulation of the potent mitogenic, motogenic, and angiogenic factor autotaxin

    SciTech Connect (OSTI)

    Song Jin; Jie Chunfa; Polk, Paula; Shridhar, Ravi; Clair, Timothy; Zhang, Jun; Yin, Lijia; Keppler, Daniel . E-mail: dkeppl@lsuhsc.edu

    2006-02-03

    We recently coined CST6 as a novel candidate tumor suppressor gene for breast cancer. CST6 indeed is expressed in the normal human breast epithelium, but little or not at all in breast carcinomas and breast cancer cell lines. Moreover, ectopic expression of CST6 in human breast cancer cells suppressed cell proliferation, migration, invasion, and orthotopic tumor growth. To obtain insights into the molecular mechanism by which CST6 exhibits its pleiotropic effects on tumor cells, we compared global gene expression profiles in mock- and CST6-transfected human MDA-MB-435S cells. Out of 12,625 transcript species, 61 showed altered expression. These included genes for extracellular matrix components, cytokines, kinases, and phosphatases, as well as several key transcription factors. TaqMan PCR assays were used to confirm the microarray data for 7 out of 11 genes. One down-regulated gene product, secreted autotaxin/lyso-phospholipase D, was of particular interest because its down-regulation by CST6 could explain most of CST6's effect on the breast cancer cells. This study thus provides First evidence that CST6 plays a role in the modulation of genes, particularly, genes that are highly relevant to breast cancer progression.

  7. Improved information analysis - views and actions

    SciTech Connect (OSTI)

    Sheely, K.B.; Manatt, D.R.

    1995-07-01

    The IAEA continues to assess, develop, and test recommendations for strengthening the cost effectiveness of safeguards. The IAEA`s investigation has focused on ways to increase Agency access to data, including access to sites and site data, export/import data, environmental data, open-source data, and other expanded data sources. Although the acquisition of this raw data is essential to strengthening safeguards, the effectiveness of the system is going to be judged by what the Agency does with the data once they have acquired it. Therefore, the IAEA must have the capability to organize, analyze and present the data in a timely manner for internal management evaluation and external dissemination. The United States Department of Energy has established a Safeguards Information Management Systems (SIMS) Initiative to provide support and equipment which will improve the IAEA`s capability to utilize this expanded data to analyze State`s nuclear activities. This paper will present views on steps to improve information analysis and discuss the status of actions undertaken by the SIMS initiative.

  8. September 2015 Most Viewed Documents for Energy Storage, Conversion...

    Office of Scientific and Technical Information (OSTI)

    September 2015 Most Viewed Documents for Energy Storage, Conversion, And Utilization ... of heavy metals: Removal from industrial wastewaters and contaminated soil ...

  9. December 2015 Most Viewed Documents for Energy Storage, Conversion...

    Office of Scientific and Technical Information (OSTI)

    December 2015 Most Viewed Documents for Energy Storage, Conversion, And Utilization ... of heavy metals: Removal from industrial wastewaters and contaminated soil ...

  10. June 2015 Most Viewed Documents for Energy Storage, Conversion...

    Office of Scientific and Technical Information (OSTI)

    June 2015 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process ... of heavy metals: Removal from industrial wastewaters and contaminated soil ...

  11. March 2016 Most Viewed Documents for Energy Storage, Conversion...

    Office of Scientific and Technical Information (OSTI)

    March 2016 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process ... of heavy metals: Removal from industrial wastewaters and contaminated soil ...

  12. BrightView Systems Ltd | Open Energy Information

    Open Energy Info (EERE)

    Ltd Jump to: navigation, search Name: BrightView Systems Ltd Place: Petach-Tikva, Israel Zip: 49510 Product: Has developed an unspecified technology for PV cell manufacture....

  13. Narrow Field of View Zenith Radiometer (NFOV) Handbook (Technical Report) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Technical Report: Narrow Field of View Zenith Radiometer (NFOV) Handbook Citation Details In-Document Search Title: Narrow Field of View Zenith Radiometer (NFOV) Handbook The two-channel narrow field-of-view radiometer (NFOV2) is a ground-based radiometer that looks straight up and measures radiance directly above the instrument at wavelengths of 673 and 870 nm. The field-of-view of the instrument is 1.2 degrees, and the sampling time resolution is one second. Measurements of

  14. July 2013 Most Viewed Documents for Chemistry | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    July 2013 Most Viewed Documents for Chemistry Science Subject Feed Lithium literature ... Grandy, J. (1997) 35 > Background chemistry for chemical warfare agents and ...

  15. March 2014 Most Viewed Documents for Chemistry | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    4 Most Viewed Documents for Chemistry Science Subject Feed Flammability characteristics of ... Richards, P. (1989) 19 > Background chemistry for chemical warfare agents and ...

  16. MediaWiki:Pagetitle-view-mainpage | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Energy Information and Data | OpenEI.org Retrieved from "http:en.openei.orgwindex.php?titleMediaWiki:Pagetitle-view-mainpage&oldid277068...

  17. January 2013 Most Viewed Documents for Chemistry | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    January 2013 Most Viewed Documents for Chemistry Dialkylenecarbonate-Bridged Polysilsesquioxanes. Hybrid Organic-Inorganic Sol-Gels with a Thermally Labile Bridging Group Assink, ...

  18. January 2013 Most Viewed Documents for Renewable Energy Sources...

    Office of Scientific and Technical Information (OSTI)

    January 2013 Most Viewed Documents for Renewable Energy Sources Photovoltaic Materials ... UV Test Methods for Encapsulants of Photovoltaic Modules (Presentation) Kempe, M. D. ...

  19. Forest View, Illinois: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Forest View, Illinois: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.8086431, -87.7933895 Show Map Loading map... "minzoom":false,"mapping...

  20. A Lidar View of Clouds in Southeastern China

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lidar View of Clouds in Southeastern China For original submission and image(s), see ARM Research Highlights http:www.arm.govsciencehighlights Research Highlight From May 2008...

  1. June 2015 Most Viewed Documents for Fission And Nuclear Technologies...

    Office of Scientific and Technical Information (OSTI)

    June 2015 Most Viewed Documents for Fission And Nuclear Technologies Estimation of gas ... MIGRATION IN SOILS OF THE CHERNOBYL NUCLEAR POWER PLANT EXCLUSION ZONE Farfan, E ...

  2. December 2015 Most Viewed Documents for Fission And Nuclear Technologi...

    Office of Scientific and Technical Information (OSTI)

    December 2015 Most Viewed Documents for Fission And Nuclear Technologies Estimation of gas ... methods and test results utilized for nuclear core applications (LWBR Development ...

  3. Most Viewed Documents for Fission and Nuclear Technologies: December...

    Office of Scientific and Technical Information (OSTI)

    Most Viewed Documents for Fission and Nuclear Technologies: December 2014 Stress analysis ... States)) (1992) 67 Behavior of spent nuclear fuel in water pool storage Johnson, A.B. ...

  4. The Better Buildings Neighborhood View - September 2012 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    September 2012 The Better Buildings Neighborhood View - September 2012 Monthly newsletter from the U.S. Department of Energy's Better Buildings Neighborhood Program. PDF icon BB...

  5. Helix Wind Inc formerly ClearView Acquisitions | Open Energy...

    Open Energy Info (EERE)

    (formerly ClearView Acquisitions) Place: San Diego, California Zip: 92113 Sector: Wind energy Product: California-based manufacturer of small scale wind turbines. References: Helix...

  6. September 2013 Most Viewed Documents for Engineering | OSTI,...

    Office of Scientific and Technical Information (OSTI)

    September 2013 Most Viewed Documents for Engineering Science Subject Feed Conduction heat transfer solutions VanSant, J.H. (1980) 190 > Charpy impact test results on five ...

  7. Mountain View Acres, California: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Mountain View Acres, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 34.496663, -117.3489352 Show Map Loading map......

  8. Mountain View, California: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Mountain View, California: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 37.3860517, -122.0838511 Show Map Loading map......

  9. Mountain View, Colorado: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Mountain View, Colorado: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.7744311, -105.0555389 Show Map Loading map... "minzoom":false,"mapp...

  10. The Better Buildings Neighborhood View -- December 2013 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications The Better Buildings Neighborhood View -- July 2013 Focus Series: Philadelphia Energyworks: In the City of Brotherly Love, Sharing Know-How Leads to ...

  11. September 2015 Most Viewed Documents for Engineering | OSTI,...

    Office of Scientific and Technical Information (OSTI)

    September 2015 Most Viewed Documents for Engineering Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 583 Charpy impact test results on five ...

  12. Better Buildings Network View | July-August 2015 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Better Buildings Network View | July-August 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View July-August 2015 More Documents & Publications Better Buildings Network View | May 2015 Better Buildings Network View | June 2015 Better Buildings Network View | April

  13. View discovery in OLAP databases through statistical combinatorial optimization

    SciTech Connect (OSTI)

    Hengartner, Nick W; Burke, John; Critchlow, Terence; Joslyn, Cliff; Hogan, Emilie

    2009-01-01

    OnLine Analytical Processing (OLAP) is a relational database technology providing users with rapid access to summary, aggregated views of a single large database, and is widely recognized for knowledge representation and discovery in high-dimensional relational databases. OLAP technologies provide intuitive and graphical access to the massively complex set of possible summary views available in large relational (SQL) structured data repositories. The capability of OLAP database software systems to handle data complexity comes at a high price for analysts, presenting them a combinatorially vast space of views of a relational database. We respond to the need to deploy technologies sufficient to allow users to guide themselves to areas of local structure by casting the space of 'views' of an OLAP database as a combinatorial object of all projections and subsets, and 'view discovery' as an search process over that lattice. We equip the view lattice with statistical information theoretical measures sufficient to support a combinatorial optimization process. We outline 'hop-chaining' as a particular view discovery algorithm over this object, wherein users are guided across a permutation of the dimensions by searching for successive two-dimensional views, pushing seen dimensions into an increasingly large background filter in a 'spiraling' search process. We illustrate this work in the context of data cubes recording summary statistics for radiation portal monitors at US ports.

  14. Using ParaView for Scientific Data Visualization | Argonne National

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory Using ParaView for Scientific Data Visualization May 16, 2016 1:00PM to 2:00PM Presenter Joseph Insley (ALCF) Location Building 401, Room A1100 Type Seminar Series APS Scientific Computation Seminar Series Abstract: ParaView is an open-source, multiplatform data analysis and visualization application. ParaView was developed to analyze extremely large datasets using distributed memory computing resources. It can be run on supercomputers to analyze large datasets as well as on

  15. DOE - NNSA/NFO -- Photo Library News & Views

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    News & Views NNSA/NFO Language Options U.S. DOE/NNSA - Nevada Field Office Photo Library - NTS 50th Anniversary Newsletter (News & Views) This special issue of the NTS News & Views is dedicated to the thousands of men and women at the Nevada National Security Site (NNSS), who for 42 years have provided this Nation with a safe, strong and effective nuclear deterrent. This issue spotlights major events at the NTS from 1951 through 1993. Instructions: Click the photograph THUMBNAIL to

  16. File and Directory Management Tool: view, organize, transfer...

    Office of Scientific and Technical Information (OSTI)

    Title: File and Directory Management Tool: view, organize, transfer files Not Specified Authors: Smith, N. G. ; O'Neill, N. J. ; Spencer, P. N. ; Long, J. W. Publication Date: ...

  17. How can I view submitted materials? | OpenEI Community

    Open Energy Info (EERE)

    Groups Menu You must login in order to post into this group. Recent content Travis Brown and Kamran Baksh, Final Submission For various reasons, you may w... How can I view...

  18. PPPL's "Star Power" video offers compelling view of Laboratory...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PPPL's "Star Power" video offers compelling view of Laboratory's research By Jeanne Jackson DeVoe September 4, 2013 Tweet Widget Google Plus One Share on Facebook PPPL has released...

  19. January 2013 Most Viewed Documents for Engineering | OSTI, US...

    Office of Scientific and Technical Information (OSTI)

    January 2013 Most Viewed Documents for Engineering Triaxial thermopile array geo-heat-flow sensor Carrigan, C.R.; Hardee, H.C.; Reynolds, G.D.; Steinfort, T.D. Critical experiments ...

  20. April 2013 Most Viewed Documents for Fission And Nuclear Technologies...

    Office of Scientific and Technical Information (OSTI)

    April 2013 Most Viewed Documents for Fission And Nuclear Technologies Science Subject Feed Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (null) 298 > ...

  1. September 2015 Most Viewed Documents for Fission And Nuclear...

    Office of Scientific and Technical Information (OSTI)

    September 2015 Most Viewed Documents for Fission And Nuclear Technologies Estimation of ... structures Chen, S.S. (1985) 167 LWR nuclear fuel bundle data for use in fuel bundle ...

  2. The Better Buildings Neighborhood View - Summer 2011 | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Summer 2011 The Better Buildings Neighborhood View - Summer 2011 The quarterly update newsletter of the Better Buildings program of the U.S. Department of Energy. PDF icon BB...

  3. West Pleasant View, Colorado: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Hide Map This article is a stub. You can help OpenEI by expanding it. West Pleasant View is a census-designated place in Jefferson County, Colorado.1 References...

  4. West View, Pennsylvania: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Hide Map This article is a stub. You can help OpenEI by expanding it. West View is a borough in Allegheny County, Pennsylvania. It falls under Pennsylvania's 4th...

  5. Mountain View Electric Association, Inc- Energy Efficiency Rebates Program

    Broader source: Energy.gov [DOE]

    Mountain View Electric Association, Inc. (MVEA) and Tri-State Generation and Transmission Association Inc., MVEA’s power supplier, offers rebates to MVEA customers who install qualifying energy...

  6. Desert View Highlands, California: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Hide Map This article is a stub. You can help OpenEI by expanding it. Desert View Highlands is a census-designated place in Los Angeles County, California.1...

  7. March 2015 Most Viewed Documents for Physics | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    5 Most Viewed Documents for Physics Plastic Gamma Sensors: An Application in Detection of ... (2003) 33 HANDBOOK OF ACCELERATOR PHYSICS AND ENGINEERING; SECTIONS 2.7.1-2.7.5 AND ...

  8. September 2015 Most Viewed Documents for Physics | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    September 2015 Most Viewed Documents for Physics Plastic Gamma Sensors: An Application in ... J.F. (1983) 38 HANDBOOK OF ACCELERATOR PHYSICS AND ENGINEERING; SECTIONS 2.7.1-2.7.5 AND ...

  9. Most Viewed Documents for Physics: December 2014 | OSTI, US Dept...

    Office of Scientific and Technical Information (OSTI)

    Most Viewed Documents for Physics: December 2014 Cathodic arc plasma deposition Anders, ... L.J. (1990) 17 HANDBOOK OF ACCELERATOR PHYSICS AND ENGINEERING; SECTIONS 2.7.1-2.7.5 AND ...

  10. Chevy Chase View, Maryland: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    is a stub. You can help OpenEI by expanding it. Chevy Chase View is a town in Montgomery County, Maryland. It falls under Maryland's 8th congressional district.12...

  11. September 2013 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    September 2013 Most Viewed Documents for Biology And Medicine Science Subject Feed Drug ... KRISHNA,C.R. (1999) 27 > Site-Directed Research and Development FY 2012 Annual Report , ...

  12. 'Art of Science' exhibition on view thru November 2012 | Princeton...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    are on view until November 2012 in the Friend Center. Xenon Plasma Accelerator Jerry Ross (fac) PPPL A picture of a Hall-effect thruster (plasma accelerator) plume. The Hall...

  13. Grid Modernization - A View from Abroad | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Grid Modernization - A View from Abroad Grid Modernization - A View from Abroad November 21, 2014 - 4:58pm Addthis Thanks for joining us for #GridWeek! | Graphic by Sarah Gerrity, Energy Department. Thanks for joining us for #GridWeek! | Graphic by Sarah Gerrity, Energy Department. Patricia A. Hoffman Patricia A. Hoffman Assistant Secretary, Office of Electricity Delivery & Energy Reliability While Energy.gov was celebrating #GridWeek this week, I traveled to Japan for the 6th International

  14. Tools for Viewing and Quality Checking ARM Data

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tools for Viewing and Quality Checking ARM Data S. Bottone and S. Moore Mission Research Corporation Santa Barbara, California Introduction Mission Research Corporation (MRC) is developing software tools to assist the Atmospheric Radiation Measurement (ARM) Data Quality Office with their data inspection tasks. One such tool is NCVweb, a web-based data analysis and visualization tool that allows for easy viewing of ARM NetCDF data files. This tool helps to eliminate the need of and problems

  15. BloombergBusiness: Viewed from space: less corn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Viewed from space: less corn Viewed from space: less corn U.S. corn production is 2.8 percent smaller than government estimates, according to a daily analysis of infrared satellite images taken of more than 1 million corn fields. September 13, 2015 Domestic corn production will be 13.34 billion bushels, Descartes Labs forecast. Source: Descartes Labs via Bloomberg Domestic corn production will be 13.34 billion bushels, Descartes Labs forecast. Source: Descartes Labs via Bloomberg Corn crop

  16. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New ALS Technique Gives Nanoscale Views of Complex Systems New ALS Technique Gives Nanoscale Views of Complex Systems Print Wednesday, 28 May 2014 00:00 Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks

  17. ALS Technique Gives Novel View of Lithium Battery Dendrite Growth

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALS Technique Gives Novel View of Lithium Battery Dendrite Growth ALS Technique Gives Novel View of Lithium Battery Dendrite Growth Print Thursday, 24 April 2014 09:46 Lithium-ion batteries, popular in today's electronic devices and electric vehicles, could gain significant energy density if their graphite anodes were replaced with lithium metal anodes. But there's a major concern with substituting lithium-when the battery cycles, microscopic fibers of the lithium anodes ("dendrites")

  18. Better Buildings Network View | July-August 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | July-August 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network. PDF icon Better Buildings Network View July-August 2014 More Documents & Publications Opportunities through the PowerSaver Loan Program Better Buildings Network View | September 2014 Better Buildings Network View | March

  19. The Honorable Gene Dean

    Office of Legacy Management (LM)

    Energy' .' Washington, DC 20585 DE! 14 1gg4 The Honorable Gene Dean \ P.O. Box 1659~ L Huntington, West Virginia 25717 _ ',.. : Dear Mayor Dean: Secretary of Energy Hazel O'Leary has'announced,a new approach ,to openness in the'Department of Energy (DOE) and its communications with the public. In support of this in.itiative, we are pleased,to forward the..enclosed information related to the former Reduction Pilot Plant sitein your jurisdiction that performed work for DOE predecessor agencies.

  20. Human AZU-1 gene, variants thereof and expressed gene products

    DOE Patents [OSTI]

    Chen, Huei-Mei; Bissell, Mina

    2004-06-22

    A human AZU-1 gene, mutants, variants and fragments thereof. Protein products encoded by the AZU-1 gene and homologs encoded by the variants of AZU-1 gene acting as tumor suppressors or markers of malignancy progression and tumorigenicity reversion. Identification, isolation and characterization of AZU-1 and AZU-2 genes localized to a tumor suppressive locus at chromosome 10q26, highly expressed in nonmalignant and premalignant cells derived from a human breast tumor progression model. A recombinant full length protein sequences encoded by the AZU-1 gene and nucleotide sequences of AZU-1 and AZU-2 genes and variant and fragments thereof. Monoclonal or polyclonal antibodies specific to AZU-1, AZU-2 encoded protein and to AZU-1, or AZU-2 encoded protein homologs.

  1. DOE Tour of Zero Floorplans: Marine Drive by Clifton View Homes |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Marine Drive by Clifton View Homes DOE Tour of Zero Floorplans: Marine Drive by Clifton View Homes DOE Tour of Zero Floorplans: Marine Drive by Clifton View

  2. DOE Tour of Zero Floorplans: Port Hadlock by Clifton View Homes |

    Energy Savers [EERE]

    Department of Energy Port Hadlock by Clifton View Homes DOE Tour of Zero Floorplans: Port Hadlock by Clifton View Homes DOE Tour of Zero Floorplans: Port Hadlock by Clifton View Homes

  3. Differing professional views or opinions: 1994 Special Review Panel

    SciTech Connect (OSTI)

    1996-09-01

    In July 1994, the Executive Director for Operations of the US Nuclear Regulatory Commission (NRC) appointed a Special Review Panel to assess the Differing Professional View or Opinion (DPV/DPO) process, including its effectiveness, how well it is understood by employees, and the organizational climate for having such views aired and properly decided. An additional area within this review was to address the effectiveness of the DPO procedures as they pertain to public access and confidentiality. Further, the Panel was charged with the review of the submittals completed since the last review to identify employees who made significant contributions to the agency or to the public health and safety but had not been adequately recognized for this contribution. The report presents the Special Review Panel`s evaluation of the NRC`s current process for dealing with Differing Professional Views or Opinions. Provided in this report are the results of an employee opinion survey on the process; highlights and suggestions from interviews with individuals who had submitted a Differing Professional View or Opinion, as well as with agency managers directly involved with the Differing Professional Views or Opinions process; and the Special Review Panel`s recommendations for improving the DPV/DPO process.

  4. V-195: RSA Authentication Manager Lets Local Users View the Administra...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5: RSA Authentication Manager Lets Local Users View the Administrative Account Password V-195: RSA Authentication Manager Lets Local Users View the Administrative Account Password...

  5. Decoding dark matter in genes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Decoding dark matter in genes Decoding dark matter in genes Possible future applications, for example, include making new cancer therapies based on how ribosomes differentiate in healthy versus cancerous tissue. February 19, 2016 Decoding dark matter in genes In 1994, researchers from Harvard and Stanford published a paper in which they described three mice: one was yellow and fat, one mottled and fat, and the last one was brown and lean. An ordinary image, except for one thing: despite being so

  6. File and Directory Management Tool: view, organize, transfer files () |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect File and Directory Management Tool: view, organize, transfer files Citation Details Software Request Title: File and Directory Management Tool: view, organize, transfer files Not Specified Authors: Smith, N. G. ; O'Neill, N. J. ; Spencer, P. N. ; Long, J. W. Publication Date: 2008-03-14 OSTI Identifier: 1231070 Report Number(s): Hopper V2.0; 002218MLTPL00 LLNL-CODE-403380 DOE Contract Number: AC52-07NA27344 Software Revision: 00 Software Package Number: 002218 Software

  7. Fermilab | Tritium at Fermilab | Indian Creek Aerial View

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Indian Creek Aerial View Indian Creek Aerial View Indian Creek is a small creek that originates on the Fermilab site and leaves the lab at its southwest corner. The flow of water in the creek varies with the amount of rain that falls during the year. At present, Indian Creek has very low levels of water. Even at its strongest flow, Indian Creek is a shallow creek with a width of a few feet where it leaves the Fermilab site. Just outside the Fermilab site, the creek flows through ponds in the

  8. Method of controlling gene expression

    DOE Patents [OSTI]

    Peters, Norman K.; Frost, John W.; Long, Sharon R.

    1991-12-03

    A method of controlling expression of a DNA segment under the control of a nod gene promoter which comprises administering to a host containing a nod gene promoter an amount sufficient to control expression of the DNA segment of a compound of the formula: ##STR1## in which each R is independently H or OH, is described.

  9. Partial growth plate closure: apex view on bone scan

    SciTech Connect (OSTI)

    Howman-Giles, R.; Trochei, M.; Yeates, K.; Middleton, R.; Barrett, I.; Scougall, J.; Whiteway, D.

    1985-01-01

    A new technique of using /sup 99m/Tc bone scan to assess partial closure of the growth plate is described. The site and degree of osseous fusion can be obtained by using the apex view. The technique has the potential of assessing serially the growth of a plate before and after surgery.

  10. Immunoglobulin λ Gene Rearrangement Can Precede κ Gene Rearrangement

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Berg, Jörg; Mcdowell, Mindy; Jäck, Hans-Martin; Wabl, Matthias

    1990-01-01

    Imore » mmunoglobulin genes are generated during differentiation of B lymphocytes by joining gene segments. A mouse pre-B cell contains a functional immunoglobulin heavy-chain gene, but no light-chain gene. Although there is only one heavy-chain locus, there are two lightchain loci: κ and λ .It has been reported that κ loci in the germ-line configuration are never (in man) or very rarely (in the mouse) present in cells with functionally rearranged λ -chain genes. Two explanations have been proposed to explain this: (a) the ordered rearrangement theory, which postulates that light-chain gene rearrangement in the pre-B cell is first attempted at the κ locus, and that only upon failure to produce a functional κ chain is there an attempt to rearrange the λ locus; and (b) the stochastic theory, which postulates that rearrangement at the λ locus proceeds at a rate that is intrinsically much slower than that at the κ locus. We show here that λ -chain genes are generated whether or not the κ locus has lost its germ-line arrangement, a result that is compatible only with the stochastic theory.« less

  11. Gene encoding herbicide safener binding protein

    DOE Patents [OSTI]

    Walton, Jonathan D.; Scott-Craig, John S.

    1999-01-01

    The cDNA encoding safener binding protein (SafBP), also referred to as SBP1, is set forth in FIG. 5 and SEQ ID No. 1. The deduced amino acid sequence is provided in FIG. 5 and SEQ ID No. 2. Methods of making and using SBP1 and SafBP to alter a plant's sensitivity to certain herbicides or a plant's responsiveness to certain safeners are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors and seeds from said plants.

  12. GenePRIMP: A GENE PRediction IMprovement Pipeline for Prokaryotic genomes

    SciTech Connect (OSTI)

    Pati, Amrita; Ivanova, Natalia N.; Mikhailova, Natalia; Ovchinnikova, Galina; Hooper, Sean D.; Lykidis, Athanasios; Kyrpides, Nikos C.

    2010-04-01

    We present 'gene prediction improvement pipeline' (GenePRIMP; http://geneprimp.jgi-psf.org/), a computational process that performs evidence-based evaluation of gene models in prokaryotic genomes and reports anomalies including inconsistent start sites, missed genes and split genes. We found that manual curation of gene models using the anomaly reports generated by GenePRIMP improved their quality, and demonstrate the applicability of GenePRIMP in improving finishing quality and comparing different genome-sequencing and annotation technologies.

  13. Narrow Field of View Zenith Radiometer (NFOV) Handbook

    SciTech Connect (OSTI)

    Chiu, C; Marshak, A; Hodges, G; Barnard, JC; Schmelzer, J

    2008-11-01

    The two-channel narrow field-of-view radiometer (NFOV2) is a ground-based radiometer that looks straight up and measures radiance directly above the instrument at wavelengths of 673 and 870 nm. The field-of-view of the instrument is 1.2 degrees, and the sampling time resolution is one second. Measurements of the NFOV2 have been used to retrieve optical properties for overhead clouds that range from patchy to overcast. With a one-second sampling rate of the NFOV2, faster than almost any other ARM Climate Research Facility (ACRF) instrument, we are able, for the first time, to capture changes in cloud optical properties at the natural time scale of cloud evolution.

  14. Metazoan Gene Families from Metazome () | Data Explorer

    Office of Scientific and Technical Information (OSTI)

    These clusters allow easy access to clade specific orthologyparalogy relationships as well as clade specific genes and gene expansions. As of version 2.0.4, Metazome provides ...

  15. March 2016 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information March 2016 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 2444 Automotive vehicle sensors Sheen, S.H.; Raptis, A.C.; Moscynski, M.J. (1995) 726 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 701 Continuously

  16. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New ALS Technique Gives Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks inside the mesoscale realm with unprecedented sensitivity and range. The new technique,

  17. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New ALS Technique Gives Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks inside the mesoscale realm with unprecedented sensitivity and range. The new technique,

  18. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New ALS Technique Gives Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks inside the mesoscale realm with unprecedented sensitivity and range. The new technique,

  19. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New ALS Technique Gives Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks inside the mesoscale realm with unprecedented sensitivity and range. The new technique,

  20. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALS Technique Gives Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks inside the mesoscale realm with unprecedented sensitivity and range. The new technique,

  1. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New ALS Technique Gives Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have difficulty simultaneously identifying and spatially resolving this realm of matter, which ranges from about 10 to 1000 nanometers in size. But ALS researchers recently developed a broadband imaging technique that looks inside the mesoscale realm with unprecedented sensitivity and range. The new technique,

  2. ALS Technique Gives Novel View of Lithium Battery Dendrite Growth

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALS Technique Gives Novel View of Lithium Battery Dendrite Growth Print Lithium-ion batteries, popular in today's electronic devices and electric vehicles, could gain significant energy density if their graphite anodes were replaced with lithium metal anodes. But there's a major concern with substituting lithium-when the battery cycles, microscopic fibers of the lithium anodes ("dendrites") form on the surface of the lithium electrode and spread across the electrolyte until they reach

  3. September 2013 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 169 Evaluation of the 2010 Toyota Prius Hybrid Synergy Drive System Burress, Timothy A [ORNL]; Campbell, Steven L [ORNL]; Coomer, Chester [ORNL]; Ayers, Curtis William [ORNL]; Wereszczak, Andrew A [ORNL]; Cunningham,

  4. September 2013 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Fission And Nuclear Technologies Science Subject Feed Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (1977) 133 /> Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste] Rezvani, M.A.; Ziada, H.H. (Westinghouse

  5. September 2013 Most Viewed Documents for Mathematics And Computing | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Mathematics And Computing Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 169 /> Lecture notes for introduction to safety and health Biele, F. (1992) 57 /> A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 50 /> Computational procedures for determining

  6. September 2015 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 1049 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 285 An Improved Method of Manufacturing Corrugated Boxes: Lateral Corrugator Frank C. Murray Ph.D.; ,

  7. September 2015 Most Viewed Documents for Environmental Sciences | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Environmental Sciences Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 285 Statistical methods for environmental pollution monitoring Gilbert, R.O. (1987) 126 Building a secondary containment system Broder, M.F. (1994) 121 Study of using oxygen-enriched combustion air for locomotive diesel engines Poola, R.B.; Sekar, R.

  8. September 2015 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Fission And Nuclear Technologies Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (1977) 444 System Definition and Analysis: Power Plant Design and Layout NONE (1996) 273 Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank

  9. September 2015 Most Viewed Documents for Mathematics And Computing | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Mathematics And Computing Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 1049 Lecture notes for introduction to safety and health Biele, F. (1992) 333 A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 286 Ferrite Measurement in Austenitic and Duplex Stainless Steel Castings -

  10. September 2015 Most Viewed Documents for Renewable Energy Sources | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Renewable Energy Sources Calculation of brine properties. [Above 80/sup 0/F and for salt content between 5 and 25%] Dittman, G.L. (1977) 257 Aerodynamic characteristics of seven symmetrical airfoil sections through 180-degree angle of attack for use in aerodynamic analysis of vertical axis wind turbines Sheldahl, R E; Klimas, P C (1981) 217 Thermal conductivity of aqueous NaCl solutions

  11. January 2013 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Energy Technology Perspectives 2012: Executive Summary [Portuguese version] NONE Energy indicators for electricity production : comparing technologies and the nature of the indicators Energy Payback Ratio (EPR), Net Energy Ratio (NER) and Cumulative Energy Demand (CED). [Oestfoldforskning AS] Raadal, Hanne Lerche [Ostfold

  12. January 2013 Most Viewed Documents for Environmental Sciences | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Environmental Sciences Horizontal Advanced Tensiometer Hubbell, Joel M.; Sisson, James B. 2 micron LIDAR for laser-based remote sensing: Flight demonstration and application survey Wagener, T.J.; Demma, N.; Kmetec, J.D.; Kubo, T.S. [Honeywell Technology Cent, Bloomington, MN (United States)] Streamflow and selected precipitation data for Yucca Mountain and vicinity, Nye County, Nevada, water

  13. January 2013 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Power Generation And Distribution Lessons from Large-Scale Renewable Energy Integration Studies: Preprint Bird, L.; Milligan, M. Small punch creep test: A promising methodology for high temperature plant components life evaluation Tettamanti, S. [CISE SpA, Milan (Italy)]; Crudeli, R. [ENEL SpA, Milan (Italy)] Failure analyses and weld repair of boiler feed water pumps Vulpen, R. van

  14. June 2014 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Energy Storage, Conversion, And Utilization Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 337 /> Seventh Edition Fuel Cell Handbook NETL (2004) 118 /> Energy Saving Potentials and Air Quality Benefits of Urban HeatIslandMitigation Akbari, Hashem (2005) 76 /> Evaluation of 2004 Toyota

  15. June 2015 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 833 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 234 An Improved Method of Manufacturing Corrugated Boxes: Lateral Corrugator Frank C. Murray Ph.D.; , Roman

  16. June 2015 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Fission And Nuclear Technologies Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (1977) 305 System Definition and Analysis: Power Plant Design and Layout NONE (1996) 296 Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste]

  17. March 2014 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Energy Storage, Conversion, And Utilization Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 291 /> Energy Saving Potentials and Air Quality Benefits of Urban HeatIslandMitigation Akbari, Hashem (2005) 85 /> Seventh Edition Fuel Cell Handbook NETL (2004) 68 /> Separation of heavy metals: Removal

  18. March 2015 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 1019 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 229 Energy Saving Potentials and Air Quality Benefits of Urban HeatIslandMitigation Akbari, Hashem (2005) 218 An

  19. March 2015 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Fission And Nuclear Technologies Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste] Rezvani, M.A.; Ziada, H.H. (Westinghouse Hanford Co., Richland, WA (United States)); Shurrab, M.S. (Westinghouse Savannah River Co., Aiken, SC (United States)) (1992) 223 Estimation of gas leak rates through very small orifices and channels. [From

  20. Most Viewed Documents for Energy Storage, Conversion, and Utilization:

    Office of Scientific and Technical Information (OSTI)

    December 2014 | OSTI, US Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Energy Storage, Conversion, and Utilization: December 2014 Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 322 Energy Saving Potentials and Air Quality Benefits of Urban HeatIslandMitigation Akbari, Hashem (2005) 107 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L.

  1. Most Viewed Documents for Environmental Sciences: December 2014 | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Environmental Sciences: December 2014 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 107 Improved Magnus` form approximation of saturation vapor pressure Alduchov, O.A.; Eskridge, R.E. [National Oceanic and Atmospheric Administration, Asheville, NC (United States). National Climatic Data Center] (1997) 50 Statistical methods for

  2. Most Viewed Documents for Fission and Nuclear Technologies: December 2014 |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Fission and Nuclear Technologies: December 2014 Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste] Rezvani, M.A.; Ziada, H.H. (Westinghouse Hanford Co., Richland, WA (United States)); Shurrab, M.S. (Westinghouse Savannah River Co., Aiken, SC (United States)) (1992) 67 Behavior of spent nuclear fuel in water pool storage Johnson,

  3. Most Viewed Documents for Mathematics and Computing: December 2014 | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Mathematics and Computing: December 2014 Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 322 Levenberg--Marquardt algorithm: implementation and theory More, J.J. (1977) 64 A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 51 Lecture notes for introduction to safety and health Biele, F. (1992) 50

  4. December 2015 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 1446 Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 452 Automotive vehicle sensors Sheen, S.H.; Raptis, A.C.; Moscynski, M.J. (1995) 373 An Improved

  5. December 2015 Most Viewed Documents for Environmental Sciences | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Environmental Sciences Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 452 Building a secondary containment system Broder, M.F. (1994) 171 Statistical methods for environmental pollution monitoring Gilbert, R.O. (1987) 116 Ammonia usage in vapor compression for refrigeration and air-conditioning in the United States

  6. December 2015 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Fission And Nuclear Technologies Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (1977) 432 System Definition and Analysis: Power Plant Design and Layout NONE (1996) 323 Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank

  7. Recombinant HT.sub.m4 gene, protein and assays

    DOE Patents [OSTI]

    Lim, Bing; Adra, Chaker N.; Lelias, Jean-Michel

    1996-01-01

    The invention relates to a recombinant DNA molecule which encodes a HT.sub.m4 protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT.sub.m4 protein and a recombinant HT.sub.m4 protein. The invention also relates to a method for detecting the presence of a hereditary atopy.

  8. The biology of novel animal genes: Mouse APEX gene knockout

    SciTech Connect (OSTI)

    MacInnes, M.; Altherr, M.R.; Ludwig, D.; Pedersen, R.; Mold, C.

    1997-07-01

    This is the final report of a one-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The controlled breeding of novel genes into mice, including the gene knockout (KO), or conversely by adding back transgenes provide powerful genetic technologies that together suffice to determine in large part the biological role(s) of novel genes. Inbred mouse remains the best understood and most useful mammalian experimental system available for tackling the biology of novel genes. The major mammalian apurinic/apyrimidinic (AP) endonuclease (APE), is involved in a key step in the repair of spontaneous and induced AP sites in DNA. Efficient repair of these lesions is imperative to prevent the stable incorporation of mutations into the cellular genome which may lead to cell death or transformation. Loss or modulation of base excison repair activity in vivo may elevate the spontaneous mutation rate in cells, and may lead to a substantial increase in the incidence of cancer. Despite extensive biochemical analysis, however, the significance of these individual APE functions in vivo has not been elucidated. Mouse embryonic stem (ES) cells heterozygous for a deletion mutation in APE have been generated and whole animals containing the APE mutation have been derived from these ES cells. Animals homozygous for the APE null mutation die early in gestation, underscoring the biological significance of this DNA repair gene.

  9. Combining Hierarchical and Associative Gene Ontology Relations with Textual Evidence in Estimating Gene and Gene Product Similarity

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Posse, Christian; Gopalan, Banu; Riensche, Roderick M.; Beagley, Nathaniel; Baddeley, Bob L.; Tratz, Stephen C.; Gregory, Michelle L.

    2007-03-01

    Gene and gene product similarity is a fundamental diagnostic measure in analyzing biological data and constructing predictive models for functional genomics. With the rising influence of the Gene Ontology, two complementary approaches have emerged where the similarity between two genes or gene products is obtained by comparing Gene Ontology (GO) annotations associated with the genes or gene products. One approach captures GO-based similarity in terms of hierarchical relations within each gene subontology. The other approach identifies GO-based similarity in terms of associative relations across the three gene subontologies. We propose a novel methodology where the two approaches can be merged with ensuing benefits in coverage and accuracy, and demonstrate that further improvements can be obtained by integrating textual evidence extracted from relevant biomedical literature.

  10. Metazoan Gene Families from Metazome

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Metazome is a joint project of the Department of Energy's Joint Genome Institute and the Center for Integrative Genomics to facilitate comparative genomic studies amongst metazoans. Clusters of orthologous and paralogous genes that represent the modern descendents of ancestral gene sets are constructed at key phylogenetic nodes. These clusters allow easy access to clade specific orthology/paralogy relationships as well as clade specific genes and gene expansions. As of version 2.0.4, Metazome provides access to twenty-four sequenced and annotated metazoan genomes, clustered at nine evolutionarily significant nodes. Where possible, each gene has been annotated with PFAM, KOG, KEGG, and PANTHER assignments, and publicly available annotations from RefSeq, UniProt, Ensembl, and JGI are hyper-linked and searchable. The included organisms (by common name) are: Human, Mouse, Rat, Dog, Opossum, Chicken, Frog, Stickleback, Medaka, Fugu pufferfish; Zebrafish, Seasquirt - savignyi, Seasquirt - intestinalis, Amphioxus, Sea Urchin, Fruitfly, Mosquite, Yellow Fever Mosquito, Silkworm, Red Flour Beetle, Worm, Briggsae Worm, Owl limpet (snail), and Sea anemone. [Copied from Metazome Overview at http://www.metazome.net/Metazome_info.php

  11. April 2013 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Science Subject Feed Seventh Edition Fuel Cell Handbook NETL (2004) 628 /> Continuously variable transmissions: theory and practice Beachley, N.H.; Frank, A.A. (null) 205 /> A study of lead-acid battery efficiency near top-of-charge and the impact on PV system design Stevens, J.W.; Corey, G.P. (1996) 173 /> Energy

  12. April 2013 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Fission And Nuclear Technologies Science Subject Feed Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (null) 298 /> Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (null) 292 /> Graphite design handbook Ho, F.H. (1988) 216 /> System Definition and

  13. April 2013 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Power Generation And Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 719 Seventh Edition Fuel Cell Handbook NETL (2004) 628 ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 343 Wet cooling towers: rule-of-thumb design and

  14. RVA: A Plugin for ParaView 3.14

    Energy Science and Technology Software Center (OSTI)

    2015-09-04

    RVA is a plugin developed for the 64-bit Windows version of the ParaView 3.14 visualization package. RVA is designed to provide support in the visualization and analysis of complex reservoirs being managed using multi-fluid EOR techniques. RVA, for Reservoir Visualization and Analysis, was developed at the University of Illinois at Urbana-Champaign, with contributions from the Illinois State Geological Survey, Department of Computer Science and National Center for Supercomputing Applications. RVA was designed to utilize andmore » enhance the state-of-the-art visualization capabilities within ParaView, readily allowing joint visualization of geologic framework and reservoir fluid simulation model results. Particular emphasis was placed on enabling visualization and analysis of simulation results highlighting multiple fluid phases, multiple properties for each fluid phase (including flow lines), multiple geologic models and multiple time steps. Additional advanced functionality was provided through the development of custom code to implement data mining capabilities. The built-in functionality of ParaView provides the capacity to process and visualize data sets ranging from small models on local desktop systems to extremely large models created and stored on remote supercomputers. The RVA plugin that we developed and the associated User Manual provide improved functionality through new software tools, and instruction in the use of ParaView-RVA, targeted to petroleum engineers and geologists in industry and research. The RVA web site (http://rva.cs.illinois.edu) provides an overview of functions, and the development web site (https://github.com/shaffer1/RVA) provides ready access to the source code, compiled binaries, user manual, and a suite of demonstration data sets. Key functionality has been included to support a range of reservoirs visualization and analysis needs, including: sophisticated connectivity analysis, cross sections through simulation results between selected wells, simplified volumetric calculations, global vertical exaggeration adjustments, ingestion of UTChem simulation results, ingestion of Isatis geostatistical framework models, interrogation of joint geologic and reservoir modeling results, joint visualization and analysis of well history files, location-targeted visualization, advanced correlation analysis, visualization of flow paths, and creation of static images and animations highlighting targeted reservoir features.« less

  15. Consumer Views on Transportation and Energy (Third Edition)

    SciTech Connect (OSTI)

    Kubik, M.

    2006-01-01

    This report has been assembled to provide the U.S. Department of Energy's (DOE's) Office of Energy Efficiency and Renewable Energy (EERE) with an idea of how the American public views various transportation, energy, and environmental issues. The data presented in this report have been drawn from multiple sources: surveys conducted by the Opinion Research Corporation (ORC) for the National Renewable Energy Laboratory (NREL) that are commissioned and funded by EERE, Gallup polls, news organization polls, surveys conducted by independent groups and academic institutions, and other sources.

  16. Ground control failures. A pictorial view of case studies

    SciTech Connect (OSTI)

    Peng, S.S.

    2007-07-01

    The book shows, in pictorial views, many forms and/or stages of types of failures in mines, for instance, cutter, roof falls, and cribs. In each case, the year of occurrence is stated in the beginning so that the environment or technological background under which it occurred are reflected. The narrative than begins with the mining and geological conditions, followed by a description of the ground control problems and recommended solutions and results, if any. The sections cover failure of pillars, roof falls, longwall, roof bolting, multiple-seam mining, floor heave, longwall, flooding and weathering of coal, old workings, and shortwall and thin-seam plow longwall.

  17. September 2013 Most Viewed Documents for Environmental Sciences | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Environmental Sciences Science Subject Feed Building a secondary containment system Broder, M.F. (1994) 59 /> Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 45 /> Test of a magnetic device for the amelioration of scale formation at Treatment Facility D Krauter, P.W., Harrar, J.E., Orloff, S.P., Bahowick, S.M. (1996)

  18. September 2013 Most Viewed Documents for Power Generation And Distribution

    Office of Scientific and Technical Information (OSTI)

    | OSTI, US Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Power Generation And Distribution Science Subject Feed Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 200 /> Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 103 /> ASPEN Plus Simulation of CO2 Recovery Process Charles

  19. September 2015 Most Viewed Documents for Power Generation And Distribution

    Office of Scientific and Technical Information (OSTI)

    | OSTI, US Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Power Generation And Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 700 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 190 Load flow analysis: Base cases, data, diagrams, and results Portante, E.C.; Kavicky,

  20. January 2013 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Fission And Nuclear Technologies Laboratory studies of shear/leach processing of zircaloy clad metallic uranium reactor fuel Swanson, J.L.; Bray, L.A.; Kjarmo, H.E.; Ryan, J.L.; Matsuzaki, C.L.; Pitman, S.G.; Haberman, J.H. Working session 3: Tubing integrity Cueto-Felgueroso, C. [Tecnatom, S.A., San Sebastian de los Reyes, Madrid (Spain)]; Strosnider, J. [NRC, Washington, DC

  1. July 2013 Most Viewed Documents for Energy Storage, Conversion, And

    Office of Scientific and Technical Information (OSTI)

    Utilization | OSTI, US Dept of Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 567 /> A study of lead-acid battery efficiency near top-of-charge and the impact on PV system design Stevens, J.W.; Corey, G.P. (1996) 142 /> Seventh Edition Fuel Cell Handbook NETL (2004) 133 />

  2. July 2013 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Fission And Nuclear Technologies Science Subject Feed Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (1977) 286 /> Graphite design handbook Ho, F.H. (1988) 136 /> Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (1977) 123 /> Stress analysis and

  3. July 2013 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Power Generation And Distribution Science Subject Feed Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 535 /> ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 165 /> Wet cooling towers: rule-of-thumb design and simulation Leeper,

  4. June 2014 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Fission And Nuclear Technologies Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (1977) 78 Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (1977) 71 Review of thorium fuel reprocessing experience Brooksbank, R.E.; McDuffee, W.T.; Rainey, R.H. (1978) 70 Stress

  5. June 2014 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Power Generation And Distribution Science Subject Feed Seventh Edition Fuel Cell Handbook NETL (2004) 118 /> Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 89 /> ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 85 /> Wet

  6. June 2015 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Power Generation And Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 504 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 240 ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 160 Load flow

  7. March 2014 Most Viewed Documents for Fission And Nuclear Technologies |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Fission And Nuclear Technologies Science Subject Feed Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (1977) 72 /> Peer-review study of the draft handbook for human-reliability analysis with emphasis on nuclear-power-plant applications, NUREG/CR-1278 Brune, R.L.; Weinstein, M.; Fitzwater, M.E. (1983) 67 /> Stress analysis and evaluation of a rectangular pressure

  8. March 2014 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Power Generation And Distribution Science Subject Feed ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 112 /> Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 83 /> Seventh Edition Fuel Cell Handbook NETL (2004) 68 /> Load flow

  9. March 2015 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Power Generation And Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 317 ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 254 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 234 Load flow analysis: Base

  10. Most Viewed Documents for Power Generation and Distribution: December 2014

    Office of Scientific and Technical Information (OSTI)

    | OSTI, US Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Power Generation and Distribution: December 2014 Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 133 Seventh Edition Fuel Cell Handbook NETL (2004) 96 ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 84 Load flow analysis: Base cases, data,

  11. December 2015 Most Viewed Documents for Mathematics And Computing | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Mathematics And Computing Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 1446 Automotive vehicle sensors Sheen, S.H.; Raptis, A.C.; Moscynski, M.J. (1995) 373 A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 365 Lecture notes for introduction to safety and health Biele, F. (1992) 324

  12. December 2015 Most Viewed Documents for Power Generation And Distribution |

    Office of Scientific and Technical Information (OSTI)

    OSTI, US Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Power Generation And Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 740 Load flow analysis: Base cases, data, diagrams, and results Portante, E.C.; Kavicky, J.A.; VanKuiken, J.C.; Peerenboom, J.P. (1997) 224 Wet cooling towers: rule-of-thumb

  13. T-704: RSA enVision Lets Remote Users View Files and Remote Authentica...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    RSA enVision Lets Remote Users View Files and Remote Authenticated Users Obtain Password T-704: RSA enVision Lets Remote Users View Files and Remote Authenticated Users Obtain...

  14. DOE Tour of Zero: The Isler Residence by Clifton View Homes ...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Isler Residence by Clifton View Homes DOE Tour of Zero: The Isler Residence by Clifton View Homes Addthis 1 of 23 This custom home built on Whidbey Island in Washington state by...

  15. A View of Environmental Justice at the U.S. Department of Energy |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy A View of Environmental Justice at the U.S. Department of Energy A View of Environmental Justice at the U.S. Department of Energy PDF icon A View of Environmental Justice at the U.S. Department of Energy More Documents & Publications A View of Environmental Justice at the U.S. Department of Energy (DOE, 2014) 2012 Independent Communication and Outreach Stakeholder Satisfaction Survey Environmental Justice Strategy

  16. Gene encoding plant asparagine synthetase

    DOE Patents [OSTI]

    Coruzzi, Gloria M.; Tsai, Fong-Ying

    1993-10-26

    The identification and cloning of the gene(s) for plant asparagine synthetase (AS), an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.

  17. Most Viewed Documents for Renewable Energy Sources: September 2014 | OSTI,

    Office of Scientific and Technical Information (OSTI)

    US Dept of Energy, Office of Scientific and Technical Information for Renewable Energy Sources: September 2014 Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 224 Advanced Electric Submersible Pump Design Tool for Geothermal Applications Xuele Qi; Norman Turnquist; Farshad Ghasripoor (2012) 179 Generalized displacement correlation method for estimating stress intensity factors Fu, P; Johnson, S M; Settgast, R R; Carrigan, C R (2011) 138 Hybrid Cooling for Geothermal Power

  18. Molecular structure in soil humic substances: The new view

    SciTech Connect (OSTI)

    Sutton, Rebecca; Sposito, Garrison

    2005-04-21

    A critical examination of published data obtained primarily from recent nuclear magnetic resonance spectroscopy, X-ray absorption near-edge structure spectroscopy, electrospray ionization-mass spectrometry, and pyrolysis studies reveals an evolving new view of the molecular structure of soil humic substances. According to the new view, humic substances are collections of diverse, relatively low molecular mass components forming dynamic associations stabilized by hydrophobic interactions and hydrogen bonds. These associations are capable of organizing into micellar structures in suitable aqueous environments. Humic components display contrasting molecular motional behavior and may be spatially segregated on a scale of nanometers. Within this new structural context, these components comprise any molecules intimately associated with a humic substance, such that they cannot be separated effectively by chemical or physical methods. Thus biomolecules strongly bound within humic fractions are by definition humic components, a conclusion that necessarily calls into question key biogeochemical pathways traditionally thought to be required for the formation of humic substances. Further research is needed to elucidate the intermolecular interactions that link humic components into supramolecular associations and to establish the pathways by which these associations emerge from the degradation of organic litter.

  19. A luciferase reporter gene assay and aryl hydrocarbon receptor 1 genotype predict the LD{sub 50} of polychlorinated biphenyls in avian species

    SciTech Connect (OSTI)

    Manning, Gillian E.; Farmahin, Reza; Environment Canada, National Wildlife Research Centre, Ottawa, ON, Canada K1A 0H3 ; Crump, Doug; Jones, Stephanie P.; Klein, Jeff; Konstantinov, Alex; Potter, Dave; Kennedy, Sean W.

    2012-09-15

    Birds differ in sensitivity to the embryotoxic effects of polychlorinated biphenyls (PCBs), which complicates environmental risk assessments for these chemicals. Recent research has shown that the identities of amino acid residues 324 and 380 in the avian aryl hydrocarbon receptor 1 (AHR1) ligand binding domain (LBD) are primarily responsible for differences in avian species sensitivity to selected dibenzo-p-dioxins and furans. A luciferase reporter gene (LRG) assay was developed in our laboratory to measure AHR1-mediated induction of a cytochrome P450 1A5 reporter gene in COS-7 cells transfected with different avian AHR1 constructs. In the present study, the LRG assay was used to measure the concentration-dependent effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and PCBs 126, 77, 105 and 118 on luciferase activity in COS-7 cells transfected with AHR1 constructs representative of 86 avian species in order to predict their sensitivity to PCB-induced embryolethality and the relative potency of PCBs in these species. The results of the LRG assay indicate that the identity of amino acid residues 324 and 380 in the AHR1 LBD are the major determinants of avian species sensitivity to PCBs. The relative potency of PCBs did not differ greatly among AHR1 constructs. Luciferase activity was significantly correlated with embryolethality data obtained from the literature (R{sup 2} ≥ 0.87, p < 0.0001). Thus, the LRG assay in combination with the knowledge of a species' AHR1 LBD sequence can be used to predict PCB-induced embryolethality in potentially any avian species of interest without the use of lethal methods on a large number of individuals. -- Highlights: ► PCB embryolethality in birds can be predicted from a species' AHR1 genotype. ► The reporter gene assay is useful for predicting species sensitivity to PCBs. ► The relative potency of PCBs does not appear to differ between AHR1 genotypes. ► Contamination of PCB 105 and PCB 118 did not affect their relative potency values.

  20. Patching genes to fight disease

    SciTech Connect (OSTI)

    Holzman, D.

    1990-09-03

    The National Institutes of Health has approved the first gene therapy experiments, one of which will try to cure cancer by bolstering the immune system. The applications of such therapy are limited, but the potential aid to people with genetic diseases is great.

  1. Blue Gene/Q Versus Blue Gene/P | Argonne Leadership Computing...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    System Overview Blue GeneQ Versus Blue GeneP BGQ Drivers Status Machine Overview Machine Partitions Torus Network Data Storage & File Systems Compiling & Linking Queueing &...

  2. Instrumentation requirements from the user's view. [For airbreathing hypersonic engines

    SciTech Connect (OSTI)

    Harsha, P.T.

    1988-01-01

    The use of combustor diagnostics is considered from the point of view of demonstration of performance of an airbreathing hypersonic engine. The basic need is seen to be that of providing the data necessary to verify performance predictions for the engine as installed in the airplane. This necessitates the use of a diagnostics capability that can provide the inputs required by the computational analyses that will be used to assess this performance. Because of the cost of ground test facilities, a premium is placed on measurement technique reliability and redundancy of instrumentation. A mix of nonintrusive optical techniques and probe-based measurements is seen to be the best approach using current diagnostics capability; one such instrument mix is outlined for a ramjet/scramjet test program. 11 references.

  3. Growth plate closure: Apex view on bone scan

    SciTech Connect (OSTI)

    Giles, P.H.; Trochei, M.; Yeates, K.

    1984-01-01

    Angular deformities of the extremities in children following premature closure of the growth plate are well known. The deformities depend on the position of an osseus bridge which forms between the epiphysis and metaphysis. Several surgical procedures including resection of the osseus bridge have been described, however, delineation of the site of fusion is difficult to define. The commonest site of growth plate arrest is the distal femoral or proximal tibial growth plate. A new technique using the bone scan has been developed which accurately defines the area and position of these osseus bridges. Two hours after injection of technetium 99m methylene diphosphonate apex views of the affected distal femoral growth plate were performed. The knee was flexed into its smallest angle. Using a pinhole collimator the gamma camera was angled to face the affected growth plate end on. The image was collected onto computer and analysed by: (I) regions of interest over segments of the growth plate to calculate the relative area of total growth plate affected: (II) generating histograms: (III) thresholding or performing isocontours to accentuate abnormal areas. The growth plate is normally uniformly increased when compared to the normal shaft of the bone. Fusion across the plate appears as an area of diminished uptake. The apex view gives a unique functional map of the growth plate such that abnormal areas are displayed, and the site, size and position of osseus fusion obtained. The technique has the potential for determining the metabolic activity of the growth plate before and after surgery. Serial studies will allow assessment of regneration of the plate and reformation of new osseus bridges.

  4. Plant nitrogen regulatory P-PII genes

    DOE Patents [OSTI]

    Coruzzi, Gloria M.; Lam, Hon-Ming; Hsieh, Ming-Hsiun

    2001-01-01

    The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the

  5. Recombinant HT{sub m4} gene, protein and assays

    DOE Patents [OSTI]

    Lim, B.; Adra, C.N.; Lelias, J.M.

    1996-09-03

    The invention relates to a recombinant DNA molecule which encodes a HT{sub m4} protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT{sub m4} protein and a recombinant HT{sub m4} protein. The invention also relates to a method for detecting the presence of a hereditary atopy. 2 figs.

  6. Method for determining gene knockouts

    DOE Patents [OSTI]

    Maranas, Costa D; Burgard, Anthony R; Pharkya, Priti

    2013-06-04

    A method for determining candidates for gene deletions and additions using a model of a metabolic network associated with an organism, the model includes a plurality of metabolic reactions defining metabolite relationships, the method includes selecting a bioengineering objective for the organism, selecting at least one cellular objective, forming an optimization problem that couples the at least one cellular objective with the bioengineering objective, and solving the optimization problem to yield at least one candidate.

  7. Method for determining gene knockouts

    DOE Patents [OSTI]

    Maranas, Costas D.; Burgard, Anthony R.; Pharkya, Priti

    2011-09-27

    A method for determining candidates for gene deletions and additions using a model of a metabolic network associated with an organism, the model includes a plurality of metabolic reactions defining metabolite relationships, the method includes selecting a bioengineering objective for the organism, selecting at least one cellular objective, forming an optimization problem that couples the at least one cellular objective with the bioengineering objective, and solving the optimization problem to yield at least one candidate.

  8. Uses of antimicrobial genes from microbial genome

    DOE Patents [OSTI]

    Sorek, Rotem; Rubin, Edward M.

    2013-08-20

    We describe a method for mining microbial genomes to discover antimicrobial genes and proteins having broad spectrum of activity. Also described are antimicrobial genes and their expression products from various microbial genomes that were found using this method. The products of such genes can be used as antimicrobial agents or as tools for molecular biology.

  9. Integrating Ontological Knowledge and Textual Evidence in Estimating Gene and Gene Product Similarity

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Posse, Christian; Gopalan, Banu; Tratz, Stephen C.; Gregory, Michelle L.

    2006-06-08

    With the rising influence of the Gene On-tology, new approaches have emerged where the similarity between genes or gene products is obtained by comparing Gene Ontology code annotations associ-ated with them. So far, these approaches have solely relied on the knowledge en-coded in the Gene Ontology and the gene annotations associated with the Gene On-tology database. The goal of this paper is to demonstrate that improvements to these approaches can be obtained by integrating textual evidence extracted from relevant biomedical literature.

  10. Thermostable cellulase from a thermomonospora gene

    DOE Patents [OSTI]

    Wilson, David B.; Walker, Larry P.; Zhang, Sheng

    1997-10-14

    The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.

  11. Thermostable cellulase from a thermomonospora gene

    DOE Patents [OSTI]

    Wilson, D.B.; Walker, L.P.; Zhang, S.

    1997-10-14

    The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity. 3 figs.

  12. Accessing and Viewing NETL Solicitations/Funding Opportunity Announcements on FedConnect

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Accessing NETL Solicitations/Funding Opportunity Announcements in FedConnect In the Internet Explorer browser, enter the FedConnect URL www.FedConnect.net On the Main Page, click on Search Public Opportunities and Awards Under Search Criteria, select Issuing Office and type in NETL. Click on Search. Click the hyperlink under the title to view. Click on Body under Solicitation to view the solicitation/announcement instructions. To view Amendments to the solicitation/announcement, click on Body

  13. Apolipoprotein gene involved in lipid metabolism

    DOE Patents [OSTI]

    Rubin, Edward; Pennacchio, Len A.

    2007-07-03

    Methods and materials for studying the effects of a newly identified human gene, APOAV, and the corresponding mouse gene apoAV. The sequences of the genes are given, and transgenic animals which either contain the gene or have the endogenous gene knocked out are described. In addition, single nucleotide polymorphisms (SNPs) in the gene are described and characterized. It is demonstrated that certain SNPs are associated with diseases involving lipids and triglycerides and other metabolic diseases. These SNPs may be used alone or with SNPs from other genes to study individual risk factors. Methods for intervention in lipid diseases, including the screening of drugs to treat lipid-related or diabetic diseases are also disclosed.

  14. T-538: HP OpenView Storage Data Protector Bug Lets Remote Users Execute Arbitrary Code

    Broader source: Energy.gov [DOE]

    A vulnerability was reported in HP OpenView Storage Data Protector. A remote user can execute arbitrary code on the target system.

  15. T-643: HP OpenView Storage Data Protector Unspecified Code Execution Vulnerability

    Broader source: Energy.gov [DOE]

    A vulnerability has been reported in HP OpenView Storage Data Protector, which can be exploited by malicious people to compromise a vulnerable system.

  16. Application of multidisciplinary analysis to gene expression.

    SciTech Connect (OSTI)

    Wang, Xuefel; Kang, Huining; Fields, Chris; Cowie, Jim R.; Davidson, George S.; Haaland, David Michael; Sibirtsev, Valeriy; Mosquera-Caro, Monica P.; Xu, Yuexian; Martin, Shawn Bryan; Helman, Paul; Andries, Erik; Ar, Kerem; Potter, Jeffrey; Willman, Cheryl L.; Murphy, Maurice H.

    2004-01-01

    Molecular analysis of cancer, at the genomic level, could lead to individualized patient diagnostics and treatments. The developments to follow will signal a significant paradigm shift in the clinical management of human cancer. Despite our initial hopes, however, it seems that simple analysis of microarray data cannot elucidate clinically significant gene functions and mechanisms. Extracting biological information from microarray data requires a complicated path involving multidisciplinary teams of biomedical researchers, computer scientists, mathematicians, statisticians, and computational linguists. The integration of the diverse outputs of each team is the limiting factor in the progress to discover candidate genes and pathways associated with the molecular biology of cancer. Specifically, one must deal with sets of significant genes identified by each method and extract whatever useful information may be found by comparing these different gene lists. Here we present our experience with such comparisons, and share methods developed in the analysis of an infant leukemia cohort studied on Affymetrix HG-U95A arrays. In particular, spatial gene clustering, hyper-dimensional projections, and computational linguistics were used to compare different gene lists. In spatial gene clustering, different gene lists are grouped together and visualized on a three-dimensional expression map, where genes with similar expressions are co-located. In another approach, projections from gene expression space onto a sphere clarify how groups of genes can jointly have more predictive power than groups of individually selected genes. Finally, online literature is automatically rearranged to present information about genes common to multiple groups, or to contrast the differences between the lists. The combination of these methods has improved our understanding of infant leukemia. While the complicated reality of the biology dashed our initial, optimistic hopes for simple answers from microarrays, we have made progress by combining very different analytic approaches.

  17. The iojap gene in maize

    SciTech Connect (OSTI)

    Martienssen, Robert

    2001-12-01

    The classical maize mutant iojap (Iodent japonica) has variegated green and white leaves. Green sectors have cells with normal chloroplasts whereas white sectors have cells where plastids fail to differentiate. These mutant plastids, when transmitted through the female gametophyte, do not recover in the presence of wild type Iojap. We cloned the Ij locus, and we have investigated the mechanism of epigenetic inheritance and phenotypic expression. More recently, a modifier of this type of variegation, ''Inhibitor of striate'', has also been cloned. Both the iojap and inhibitor of striate proteins have homologs in bacteria and are members of ancient conserved families found in multiple species. These tools can be used to address fundamental questions of inheritance and variegation associated with this classical conundrum of maize genetics. Since the work of Rhoades there has been considerable speculation concerning the nature of the Iojap gene product, the origin of leaf variegation and the mechanism behind the material inheritance of defective plastids. This has made Iojap a textbook paradigm for cytoplasmic inheritance and nuclear-organellar interaction for almost 50 years. Cloning of the Iojap gene in maize, and homologs in other plants and bacteria, provides a new means to address the origin of heteroplastidity, variegation and cytoplasmic inheritance in higher plants.

  18. Imaging gene expression in real-time using aptamers

    SciTech Connect (OSTI)

    Shin, Il Chung

    2011-12-13

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging microscopy). Real-time transcription was measured by FLIM-FRET, which was detected by the decrease in donor lifetime resulting from ligand binding to IMAGEtags that were newly synthesized from the activated GAL1 promoter. The FRET signal was specific for transcribed IMAGEtags.

  19. Methods for monitoring multiple gene expression

    DOE Patents [OSTI]

    Berka, Randy; Bachkirova, Elena; Rey, Michael

    2013-10-01

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  20. Methods for monitoring multiple gene expression

    DOE Patents [OSTI]

    Berka, Randy; Bachkirova, Elena; Rey, Michael

    2008-06-01

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  1. Methods for monitoring multiple gene expression

    DOE Patents [OSTI]

    Berka, Randy; Bachkirova, Elena; Rey, Michael

    2012-05-01

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  2. Cross-Ontological Analytics: Combining Associative and Hierarchical Relations in the Gene Ontologies to Assess Gene Product Similarity

    SciTech Connect (OSTI)

    Posse, Christian; Sanfilippo, Antonio P.; Gopalan, Banu; Riensche, Roderick M.; Beagley, Nathaniel; Baddeley, Bob L.

    2006-05-28

    Gene and gene product similarity is a fundamental diagnostic measure in analyzing biological data and constructing predictive models for functional genomics. With the rising influence of the gene ontologies, two complementary approaches have emerged where the similarity between two genes/gene products is obtained by comparing gene ontology (GO) annotations associated with the gene/gene products. One approach captures GO-based similarity in terms of hierarchical relations within each gene ontology. The other approach identifies GO-based similarity in terms of associative relations across the three gene ontologies. We propose a novel methodology where the two approaches can be merged with ensuing benefits in coverage and accuracy.

  3. Gene coding for the E1 endoglucanase

    DOE Patents [OSTI]

    Thomas, S.R.; Laymon, R.A.; Himmel, M.E.

    1996-07-16

    The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol. 6 figs.

  4. HMM-Based Gene Annotation Methods

    SciTech Connect (OSTI)

    Haussler, David; Hughey, Richard; Karplus, Keven

    1999-09-20

    Development of new statistical methods and computational tools to identify genes in human genomic DNA, and to provide clues to their functions by identifying features such as transcription factor binding sites, tissue, specific expression and splicing patterns, and remove homologies at the protein level with genes of known function.

  5. Gene coding for the E1 endoglucanase

    DOE Patents [OSTI]

    Thomas, Steven R.; Laymon, Robert A.; Himmel, Michael E.

    1996-01-01

    The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol.

  6. View the Apps for Vehicles Phase One Submissions | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    View the Apps for Vehicles Phase One Submissions View the Apps for Vehicles Phase One Submissions January 24, 2013 - 11:13am Addthis 9 of the 37 entries for the Apps for Vehicles competition. 9 of the 37 entries for the Apps for Vehicles competition. Ian Kalin Director of the Energy Data Initiative How can I participate? Visit the competition website to view the submitted ideas. Starting on March 15, you'll be able to vote for your favorite completed apps. Apps for Vehicles The Open Data

  7. DOE Tour of Zero: Marine Drive by Clifton View Homes | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Marine Drive by Clifton View Homes DOE Tour of Zero: Marine Drive by Clifton View Homes Addthis 1 of 10 Clifton View Homes built this 2,408-square-foot zero energy home in Coupeville, Washington, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 10 The ultra-efficient home has 7.84 kW of solar electric panels on the roof, which contribute to zero utility bills for the homeowners. The roof is built with structural insulated panels (SIPs) that

  8. DOE Tour of Zero: Marine Drive by Clifton View Homes | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Marine Drive by Clifton View Homes DOE Tour of Zero: Marine Drive by Clifton View Homes 1 of 10 Clifton View Homes built this 2,408-square-foot zero energy home in Coupeville, Washington, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 10 The ultra-efficient home has 7.84 kW of solar electric panels on the roof, which contribute to zero utility bills for the homeowners. The roof is built with structural insulated panels (SIPs) that are

  9. DOE Tour of Zero: Port Hadlock by Clifton View Homes | Department of Energy

    Energy Savers [EERE]

    Port Hadlock by Clifton View Homes DOE Tour of Zero: Port Hadlock by Clifton View Homes Addthis 1 of 10 Clifton View Homes built this 1,784-square-foot zero energy home in Port Hadlock, Washington, to the performance criteria of the U.S. Department of Energy Zero Energy Ready Home (ZERH) program. 2 of 10 The ultra-efficient home has 7.56 kW of solar electric panels on the roof that help it to achieve annual energy bills of $35. The solar panels are installed on the durable, fire-resistant

  10. Optic for an endoscope/borescope having high resolution and narrow field of view

    DOE Patents [OSTI]

    Stone, Gary F.; Trebes, James E.

    2003-10-28

    An optic having optimized high spatial resolution, minimal nonlinear magnification distortion while at the same time having a limited chromatic focal shift or chromatic aberrations. The optic located at the distal end of an endoscopic inspection tool permits a high resolution, narrow field of view image for medical diagnostic applications, compared to conventional optics for endoscopic instruments which provide a wide field of view, low resolution image. The image coverage is over a narrow (<20 degrees) field of view with very low optical distortion (<5% pin cushion or barrel distortion. The optic is also optimized for best color correction as well as to aid medical diagnostics.

  11. View TEDxCERN in Jefferson Lab Auditorium on Sept. 24 | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    View TEDxCERN in Jefferson Lab Auditorium on Sept. 24 View TEDxCERN in Jefferson Lab Auditorium on Sept. 24 NEWPORT NEWS, Va. - Jefferson Lab will stream TEDxCERN 2014 on the big screen in the CEBAF Center auditorium on Sept. 24, from 7:30 a.m. - 1:30 p.m. EDT. University and college faculty and students affiliated with Jefferson Lab are invited to attend all or any part of the event viewing. TED talks are brief conversations - each lasting 18 minutes or less - that bring together people from

  12. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  13. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wild 2 Viewed by ALS Microscopes Print NASA's 200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81PWild...

  14. DOE Zero Energy Ready Home Case Study: Clifton View Homes, Coupeville...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE Zero Energy Ready Home Case Study: Clifton View Homes, Coupeville, WA, Systems Home Case study of a DOE Zero Energy Ready Home on Whidbey Island, WA, that scored HERS 45 ...

  15. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81PWild 2 Viewed by ALS Microscopes Print NASA's 200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the...

  16. GPU-based Scalable Volumetric Reconstruction for Multi-view Stereo

    SciTech Connect (OSTI)

    Kim, H; Duchaineau, M; Max, N

    2011-09-21

    We present a new scalable volumetric reconstruction algorithm for multi-view stereo using a graphics processing unit (GPU). It is an effectively parallelized GPU algorithm that simultaneously uses a large number of GPU threads, each of which performs voxel carving, in order to integrate depth maps with images from multiple views. Each depth map, triangulated from pair-wise semi-dense correspondences, represents a view-dependent surface of the scene. This algorithm also provides scalability for large-scale scene reconstruction in a high resolution voxel grid by utilizing streaming and parallel computation. The output is a photo-realistic 3D scene model in a volumetric or point-based representation. We demonstrate the effectiveness and the speed of our algorithm with a synthetic scene and real urban/outdoor scenes. Our method can also be integrated with existing multi-view stereo algorithms such as PMVS2 to fill holes or gaps in textureless regions.

  17. June 2014 Most Viewed Documents for Physics | OSTI, US Dept of...

    Office of Scientific and Technical Information (OSTI)

    June 2014 Most Viewed Documents for Physics Science Subject Feed Plastic Gamma Sensors: An ... (1983) 25 > HANDBOOK OF ACCELERATOR PHYSICS AND ENGINEERING; SECTIONS 2.7.1-2.7.5 AND ...

  18. June 2015 Most Viewed Documents for Physics | OSTI, US Dept of...

    Office of Scientific and Technical Information (OSTI)

    June 2015 Most Viewed Documents for Physics Cathodic arc plasma deposition Anders, Andre ... M. (1991) 38 HANDBOOK OF ACCELERATOR PHYSICS AND ENGINEERING; SECTIONS 2.7.1-2.7.5 AND ...

  19. July 2013 Most Viewed Documents for Physics | OSTI, US Dept of...

    Office of Scientific and Technical Information (OSTI)

    July 2013 Most Viewed Documents for Physics Science Subject Feed Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; Chou, ...

  20. DOE Zero Energy Ready Home Case Study: Clifton View Homes, Marine...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and passive design; air-to-water heatpump, fresh air intake with fan, triple-pane windows, 100% LED. PDF icon DOE Zero Energy Ready Home Case Study: Clifton View Homes, ...

  1. A View of Environmental Justice at the U.S. Department of Energy (DOE,

    Energy Savers [EERE]

    2014) | Department of Energy A View of Environmental Justice at the U.S. Department of Energy (DOE, 2014) A View of Environmental Justice at the U.S. Department of Energy (DOE, 2014) Executive Order 12898 requires each federal agency to make environmental justice part of its mission by identifying and addressing, as appropriate, disproportionally high and adverse human health or environmental effects of its program, policies, and activities on minority populations and low-income populations.

  2. New Window Technology Saves Energy and the View | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Window Technology Saves Energy and the View New Window Technology Saves Energy and the View November 5, 2013 - 3:55pm Addthis Researchers at the Energy Department's National Renewable Energy Laboratory are developing innovative new window technology that helps improve occupants' comfort and cuts energy use. | Photo courtesy of Pat Corkery, NREL. Researchers at the Energy Department's National Renewable Energy Laboratory are developing innovative new window technology that helps improve

  3. DOE Seeks Public Views On How Best To Streamline Existing Regulations |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Public Views On How Best To Streamline Existing Regulations DOE Seeks Public Views On How Best To Streamline Existing Regulations January 31, 2011 - 5:53pm Addthis The Department of Energy today announced two immediate steps to implement the Obama Administration's Executive Order on Improving Regulation and Regulatory Review, which directs federal agencies to review existing regulations and determine whether they are still necessary and crafted effectively to solve

  4. Microsoft Word - A Systems View of the Modern Grid_Final_v2_0.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    V2.0 The NETL Modern Grid Initiative A SYSTEMS VIEW OF THE MODERN GRID Conducted by the National Energy Technology Laboratory for the U.S. Department of Energy Office of Electricity Delivery and Energy Reliability January 2007 Office of Electricity Delivery and Energy Reliability Page 1 v2.0 A Systems View of the Modern Grid TABLE OF CONTENTS Table of Contents ..................................................................................... 1 Executive Summary

  5. Viewing spin structures with soft x-ray microscopy (Journal Article) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Viewing spin structures with soft x-ray microscopy Citation Details In-Document Search Title: Viewing spin structures with soft x-ray microscopy The spin of the electron and its associated magnetic moment marks the basic unit for magnetic properties of matter. Magnetism, in particular ferromagnetism and antiferromagnetism is described by a collective order of these spins, where the interaction between individual spins reflects a competition between exchange, anisotropy and

  6. Biogas From Municipal WWTPs: Fuel Cells Viewed as a Value Proposition |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy From Municipal WWTPs: Fuel Cells Viewed as a Value Proposition Biogas From Municipal WWTPs: Fuel Cells Viewed as a Value Proposition Presentation about the value proposition for biogas from waste water treatment plants. Presented by Steve Hamilton, SCS Energy, at the NREL/DOE Biogas and Fuel Cells Workshop held June 11-13, 2012, in Golden, Colorado. PDF icon june2012_biogas_workshop_hamilton.pdf More Documents & Publications Biogas and Fuel Cells Workshop Summary

  7. Dynamics Modelling of Biolistic Gene Guns

    SciTech Connect (OSTI)

    Zhang, M.; Tao, W.; Pianetta, P.A.

    2009-06-04

    The gene transfer process using biolistic gene guns is a highly dynamic process. To achieve good performance, the process needs to be well understood and controlled. Unfortunately, no dynamic model is available in the open literature for analysing and controlling the process. This paper proposes such a model. Relationships of the penetration depth with the helium pressure, the penetration depth with the acceleration distance, and the penetration depth with the micro-carrier radius are presented. Simulations have also been conducted. The results agree well with experimental results in the open literature. The contribution of this paper includes a dynamic model for improving and manipulating performance of the biolistic gene gun.

  8. Uses of antimicrobial genes from microbial genome Sorek, Rotem...

    Office of Scientific and Technical Information (OSTI)

    We describe a method for mining microbial genomes to discover antimicrobial genes and proteins having broad spectrum of activity. Also described are antimicrobial genes and their...

  9. Gene Geracao Eolica Do Nordeste | Open Energy Information

    Open Energy Info (EERE)

    Gene Geracao Eolica Do Nordeste Jump to: navigation, search Name: Gene - Geracao Eolica Do Nordeste Place: Brazil Sector: Wind energy Product: Aims to manufacture and commercialize...

  10. Visualization and Analysis of 3D Gene Expression Data (Technical...

    Office of Scientific and Technical Information (OSTI)

    Technical Report: Visualization and Analysis of 3D Gene Expression Data Citation Details In-Document Search Title: Visualization and Analysis of 3D Gene Expression Data Recent...

  11. (for Gene Recognition Analysis Internet Link),

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Scientific Impact: About 1,000 biotechnology companies and laboratories now use GRAIL to track down genes that play a role in human disease. The program has become increasingly ...

  12. Gene expression profiles in irradiated cancer cells

    SciTech Connect (OSTI)

    Minafra, L.; Bravat, V.; Russo, G.; Ripamonti, M.; Gilardi, M. C.

    2013-07-26

    Knowledge of the molecular and genetic mechanisms underlying cellular response to radiation may provide new avenues to develop innovative predictive tests of radiosensitivity of tumours and normal tissues and to improve individual therapy. Nowadays very few studies describe molecular changes induced by hadrontherapy treatments, therefore this field has to be explored and clarified. High-throughput methodologies, such as DNA microarray, allow us to analyse mRNA expression of thousands of genes simultaneously in order to discover new genes and pathways as targets of response to hadrontherapy. Our aim is to elucidate the molecular networks involved in the sensitivity/resistance of cancer cell lines subjected to hadrontherapy treatments with a genomewide approach by using cDNA microarray technology to identify gene expression profiles and candidate genes responsible of differential cellular responses.

  13. Mapping genes to human chromosome 19

    SciTech Connect (OSTI)

    Connolly, Sarah

    1996-05-01

    For this project, 22 Expressed Sequence Tags (ESTs) were fine mapped to regions of human chromosome 19. An EST is a short DNA sequence that occurs once in the genome and corresponds to a single expressed gene. {sup 32}P-radiolabeled probes were made by polymerase chain reaction for each EST and hybridized to filters containing a chromosome 19-specific cosmid library. The location of the ESTs on the chromosome was determined by the location of the ordered cosmid to which the EST hybridized. Of the 22 ESTs that were sublocalized, 6 correspond to known genes, and 16 correspond to anonymous genes. These localized ESTs may serve as potential candidates for disease genes, as well as markers for future physical mapping.

  14. Changes in gene expression following EMF exposure

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Chang-Liu, C.M.; Loberg, L.; Gauger, J.; McCormick, D.

    1997-10-01

    Experiments were designed to examine the effects of electromagnetic field (EMF) exposure on specific gene expression, an effect that can be deleterious, beneficial, or neutral, depending on the long-term consequences; however, the proof of a reproducible, quantitative biological effect (such as change in gene expression) will lead to latter experiments aimed at determining the relative contribution of these changes to cellular consequences. Past work by ourselves and by others has shown that measures of gene expression are extremely sensitive indicators of the cellular and biological effects of ionizing radiation, with transcriptional changes being detected by exposure of cells to doses of {gamma}-rays as low as 0.01 cGy that have no pronounced cellular consequences. On the basis of this work, the authors hypothesized that measures of gene expression will be equally sensitive to EMF effects on cells.

  15. Non-coplanar polychlorinated biphenyls (PCBs) are direct agonists for the human pregnane-X receptor and constitutive androstane receptor, and activate target gene expression in a tissue-specific manner

    SciTech Connect (OSTI)

    Al-Salman, Fadheela; Plant, Nick

    2012-08-15

    The polychlorinated biphenyl group possesses high environmental persistence, leading to bioaccumulation and a number of adverse effects in mammals. Whilst coplanar PCBs elicit their toxic effects through agonism of the aryl hydrocarbon receptor; however, non-coplanar PCBs are not ligands for AhR, but may be ligands for members of the nuclear receptor family of proteins. To better understand the biological actions of non-coplanar PCBs, we have undertaken a systematic analysis of their ability to activate PXR and CAR-mediated effects. Cells were exposed to a range of non-coplanar PCBs (99, 138, 153, 180 and 194), or the coplanar PCB77: Direct activation of PXR and CAR was measured using a mammalian receptor activation assay in human liver cells, with rifampicin and CITCO used as positive controls ligands for PXR and CAR, respectively; activation of target gene expression was examined using reporter gene plasmids for CYP3A4 and MDR1 transfected into liver, intestine and lung cell lines. Several of the non-coplanar PCBs directly activated PXR and CAR, whilst the coplanar PCB77 did not. Non-coplanar PCBs were also able to activate PXR/CAR target gene expression in a substitution- and tissue-specific manner. Non-coplanar PCBs act as direct activators for the nuclear receptors PXR and CAR, and are able to elicit transcriptional activation of target genes in a substitution- and tissue-dependent manner. Chronic activation of PXR/CAR is linked to adverse effects and must be included in any risk assessment of PCBs. -- Highlights: ► Several Non-coplanar PCBs are able to directly activate both PXR and CAR in vitro. ► PCB153 is the most potent direct activator of PXR and CAR nuclear receptors. ► Non-coplanar PCB activation of CYP3A4/MDR1 reporter genes is structure-dependent. ► Non-coplanar PCB activate CYP3A4/MDR1 reporter genes in a tissue-dependent. ► PCB153 is the most potent activator of PXR/CAR target gene in all tissues.

  16. Blue Gene/Q | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Blue Gene/Q Download original image « Back to galleryItem 4

  17. Gene for ataxia-telangiectasia complementation group D (ATDC)

    DOE Patents [OSTI]

    Murnane, J.P.; Painter, R.B.; Kapp, L.N.; Yu, L.C.

    1995-03-07

    Disclosed herein is a new gene, an AT gene for complementation group D, the ATDC gene and fragments thereof. Nucleic acid probes for the gene are provided as well as proteins encoded by the gene, cDNA therefrom, preferably a 3 kilobase (kb) cDNA, and recombinant nucleic acid molecules for expression of the proteins. Further disclosed are methods to detect mutations in the gene, preferably methods employing the polymerase chain reaction (PCR). Also disclosed are methods to detect AT genes from other AT complementation groups. 30 figs.

  18. Gene for ataxia-telangiectasia complementation group D (ATDC)

    DOE Patents [OSTI]

    Murnane, John P.; Painter, Robert B.; Kapp, Leon N.; Yu, Loh-Chung

    1995-03-07

    Disclosed herein is a new gene, an AT gene for complementation group D, the ATDC gene and fragments thereof. Nucleic acid probes for said gene are provided as well as proteins encoded by said gene, cDNA therefrom, preferably a 3 kilobase (kb) cDNA, and recombinant nucleic acid molecules for expression of said proteins. Further disclosed are methods to detect mutations in said gene, preferably methods employing the polymerase chain reaction (PCR). Also disclosed are methods to detect AT genes from other AT complementation groups.

  19. Measuring semantic similarities by combining gene ontology annotations and gene co-function networks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Peng, Jiajie; Uygun, Sahra; Kim, Taehyong; Wang, Yadong; Rhee, Seung Y.; Chen, Jin

    2015-02-14

    Background: Gene Ontology (GO) has been used widely to study functional relationships between genes. The current semantic similarity measures rely only on GO annotations and GO structure. This limits the power of GO-based similarity because of the limited proportion of genes that are annotated to GO in most organisms. Results: We introduce a novel approach called NETSIM (network-based similarity measure) that incorporates information from gene co-function networks in addition to using the GO structure and annotations. Using metabolic reaction maps of yeast, Arabidopsis, and human, we demonstrate that NETSIM can improve the accuracy of GO term similarities. We also demonstratemore » that NETSIM works well even for genomes with sparser gene annotation data. We applied NETSIM on large Arabidopsis gene families such as cytochrome P450 monooxygenases to group the members functionally and show that this grouping could facilitate functional characterization of genes in these families. Conclusions: Using NETSIM as an example, we demonstrated that the performance of a semantic similarity measure could be significantly improved after incorporating genome-specific information. NETSIM incorporates both GO annotations and gene co-function network data as a priori knowledge in the model. Therefore, functional similarities of GO terms that are not explicitly encoded in GO but are relevant in a taxon-specific manner become measurable when GO annotations are limited.« less

  20. Key gene regulating cell wall biosynthesis and recalcitrance in Populus, gene Y

    DOE Patents [OSTI]

    Chen, Jay; Engle, Nancy; Gunter, Lee E.; Jawdy, Sara; Tschaplinski, Timothy J.; Tuskan, Gerald A.

    2015-12-08

    This disclosure provides methods and transgenic plants for improved production of renewable biofuels and other plant-derived biomaterials by altering the expression and/or activity of Gene Y, an O-acetyltransferase. This disclosure also provides expression vectors containing a nucleic acid (Gene Y) which encodes the polypeptide of SEQ ID NO: 1 and is operably linked to a heterologous promoter.

  1. Measuring semantic similarities by combining gene ontology annotations and gene co-function networks

    SciTech Connect (OSTI)

    Peng, Jiajie; Uygun, Sahra; Kim, Taehyong; Wang, Yadong; Rhee, Seung Y.; Chen, Jin

    2015-02-14

    Background: Gene Ontology (GO) has been used widely to study functional relationships between genes. The current semantic similarity measures rely only on GO annotations and GO structure. This limits the power of GO-based similarity because of the limited proportion of genes that are annotated to GO in most organisms. Results: We introduce a novel approach called NETSIM (network-based similarity measure) that incorporates information from gene co-function networks in addition to using the GO structure and annotations. Using metabolic reaction maps of yeast, Arabidopsis, and human, we demonstrate that NETSIM can improve the accuracy of GO term similarities. We also demonstrate that NETSIM works well even for genomes with sparser gene annotation data. We applied NETSIM on large Arabidopsis gene families such as cytochrome P450 monooxygenases to group the members functionally and show that this grouping could facilitate functional characterization of genes in these families. Conclusions: Using NETSIM as an example, we demonstrated that the performance of a semantic similarity measure could be significantly improved after incorporating genome-specific information. NETSIM incorporates both GO annotations and gene co-function network data as a priori knowledge in the model. Therefore, functional similarities of GO terms that are not explicitly encoded in GO but are relevant in a taxon-specific manner become measurable when GO annotations are limited.

  2. Pathogenicity island mobility and gene content.

    SciTech Connect (OSTI)

    Williams, Kelly Porter

    2013-10-01

    Key goals towards national biosecurity include methods for analyzing pathogens, predicting their emergence, and developing countermeasures. These goals are served by studying bacterial genes that promote pathogenicity and the pathogenicity islands that mobilize them. Cyberinfrastructure promoting an island database advances this field and enables deeper bioinformatic analysis that may identify novel pathogenicity genes. New automated methods and rich visualizations were developed for identifying pathogenicity islands, based on the principle that islands occur sporadically among closely related strains. The chromosomally-ordered pan-genome organizes all genes from a clade of strains; gaps in this visualization indicate islands, and decorations of the gene matrix facilitate exploration of island gene functions. A %E2%80%9Clearned phyloblocks%E2%80%9D method was developed for automated island identification, that trains on the phylogenetic patterns of islands identified by other methods. Learned phyloblocks better defined termini of previously identified islands in multidrug-resistant Klebsiella pneumoniae ATCC BAA-2146, and found its only antibiotic resistance island.

  3. Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats

    SciTech Connect (OSTI)

    Marín-Prida, Javier; Riva, Federica; Pentón-Arias, Eduardo

    2013-10-01

    Since the inflammatory response and oxidative stress are involved in the stroke cascade, we evaluated here the effects of Phycocyanobilin (PCB, the C-Phycocyanin linked tetrapyrrole) on PC12 cell survival, the gene expression and the oxidative status of hypoperfused rat brain. After the permanent bilateral common carotid arteries occlusion (BCCAo), the animals were treated with saline or PCB, taking samples 24 h post-surgery. Global gene expression was analyzed with GeneChip Rat Gene ST 1.1 from Affymetrix; the expression of particular genes was assessed by the Fast SYBR Green RT-PCR Master Mix and Bioplex methods; and redox markers (MDA, PP, CAT, SOD) were evaluated spectrophotometrically. The PCB treatment prevented the H{sub 2}O{sub 2} and glutamate induced PC12 cell injury assessed by the MTT assay, and modulated 190 genes (93 up- and 97 down-regulated) associated to several immunological and inflammatory processes in BCCAo rats. Furthermore, PCB positively modulated 19 genes mostly related to a detrimental pro-inflammatory environment and counteracted the oxidative imbalance in the treated BCCAo animals. Our results support the view of an effective influence of PCB on major inflammatory mediators in acute cerebral hypoperfusion. These results suggest that PCB has a potential to be a treatment for ischemic stroke for which further studies are needed. - Highlights: • Phycocyanobilin (PCB) prevents H{sub 2}O{sub 2} and glutamate induced PC12 cell viability loss. • Anterior cortex and striatum are highly vulnerable to cerebral hypoperfusion (CH). • PCB modulates 190 genes associated to inflammation in acute CH. • PCB regulates 19 genes mostly related to a detrimental pro-inflammatory environment. • PCB restores redox and immune balances showing promise as potential stroke therapy.

  4. Under-Sodium Viewing: A Review of Ultrasonic Imaging Technology for Liquid Metal Fast Reactors

    SciTech Connect (OSTI)

    Griffin, Jeffrey W.; Peters, Timothy J.; Posakony, Gerald J.; Chien, Hual-Te; Bond, Leonard J.; Denslow, Kayte M.; Sheen, Shuh-Haw; Raptis, Paul

    2009-03-27

    This current report is a summary of information obtained in the "Information Capture" task of the U.S. DOE-funded "Under Sodium Viewing (USV) Project." The goal of the multi-year USV project is to design, build, and demonstrate a state-of-the-art prototype ultrasonic viewing system tailored for periodic reactor core in-service monitoring and maintenance inspections. The study seeks to optimize system parameters, improve performance, and re-establish this key technology area which will be required to support any new U.S. liquid-metal cooled fast reactors.

  5. Experiments at Scale with In-Situ Visualization Using ParaView/Catalyst in RAGE

    SciTech Connect (OSTI)

    Kares, Robert John

    2014-10-31

    In this paper I describe some numerical experiments performed using the ParaView/Catalyst in-situ visualization infrastructure deployed in the Los Alamos RAGE radiation-hydrodynamics code to produce images from a running large scale 3D ICF simulation on the Cielo supercomputer at Los Alamos. The detailed procedures for the creation of the visualizations using ParaView/Catalyst are discussed and several images sequences from the ICF simulation problem produced with the in-situ method are presented. My impressions and conclusions concerning the use of the in-situ visualization method in RAGE are discussed.

  6. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print Wednesday, 30 May 2007 00:00 NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination

  7. Jefferson Lab scientist puts intriguing view on spin of the proton |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jefferson Lab scientist puts intriguing view on spin of the proton Jefferson Lab scientist puts intriguing view on spin of the proton April 17, 2007 Newport News, Va. - A new result from the HERMES experiment at The German Electron Synchrotron DESY indicates that the gluons which bind quarks into the proton show only slight polarization. The result will be presented by Elke-Caroline Aschenauer, a former HERMES spokesperson and current 12 GeV Upgrade Hall D Leader at the Department of

  8. Capella Corona Revisited: a Combined View from Textit XMM-Newton RGS,

    Office of Scientific and Technical Information (OSTI)

    Textit Chandra HETGS, and LETGS (Conference) | SciTech Connect Conference: Capella Corona Revisited: a Combined View from Textit XMM-Newton RGS, Textit Chandra HETGS, and LETGS Citation Details In-Document Search Title: Capella Corona Revisited: a Combined View from Textit XMM-Newton RGS, Textit Chandra HETGS, and LETGS We present a combined analysis of the X-ray emission of the Capella corona obtained with XMM-Newton RGS, Chandra HETGS, and LETGS. An improved atomic line database and a new

  9. Capella Corona Revisited: a Combined View from Textit XMM-Newton RGS,

    Office of Scientific and Technical Information (OSTI)

    Textit Chandra HETGS, and LETGS (Conference) | SciTech Connect Conference: Capella Corona Revisited: a Combined View from Textit XMM-Newton RGS, Textit Chandra HETGS, and LETGS Citation Details In-Document Search Title: Capella Corona Revisited: a Combined View from Textit XMM-Newton RGS, Textit Chandra HETGS, and LETGS × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI)

  10. A space-based, high-resolution view of notable changes in urban NO x

    Office of Scientific and Technical Information (OSTI)

    pollution around the world (2005-2014) (Journal Article) | SciTech Connect A space-based, high-resolution view of notable changes in urban NO x pollution around the world (2005-2014) Citation Details In-Document Search Title: A space-based, high-resolution view of notable changes in urban NO x pollution around the world (2005-2014) Nitrogen oxides (NOx = NO + NO2) are produced during combustion processes and, thus may serve as a proxy for fossil fuel-based energy usage and coemitted

  11. Prairie View A&M University | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Design Competition | Department of Energy Prairie View A&M Tops 2016 U.S. Department of Energy Race to Zero Student Design Competition Prairie View A&M Tops 2016 U.S. Department of Energy Race to Zero Student Design Competition April 22, 2016 - 3:00pm Addthis As part of the Obama Administration's commitment to helping families across the United States save money by saving energy, the U.S. Department of Energy announced winners of its third annual Race to Zero Student Design

  12. New methods for tightly regulated gene expression and highly efficient

    Office of Scientific and Technical Information (OSTI)

    chromosomal integration of cloned genes for Methanosarcina species (Journal Article) | SciTech Connect New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species Citation Details In-Document Search Title: New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species A highly efficient method for chromosomal integration of cloned DNA into

  13. Universal light-switchable gene promoter system

    DOE Patents [OSTI]

    Quail, Peter H.; Huq, Enamul; Tepperman, James; Sato, Sae

    2005-02-22

    An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.

  14. Id-1 gene and gene products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    DOE Patents [OSTI]

    Desprez, Pierre-Yves; Campisi, Judith

    2014-08-19

    A method for treatment of breast cancer and other types of cancer. The method comprises targeting and modulating Id-1 gene expression, if any, for the Id-1 gene, or gene products in breast or other epithelial cancers in a patient by delivering products that modulate Id-1 gene expression. When expressed, Id-1 gene is a prognostic indicator that cancer cells are invasive and metastatic.

  15. Optic for industrial endoscope/borescope with narrow field of view and low distortion

    DOE Patents [OSTI]

    Stone, Gary F.; Trebes, James E.

    2005-08-16

    An optic for the imaging optics on the distal end of a flexible fiberoptic endoscope or rigid borescope inspection tool. The image coverage is over a narrow (<20 degrees) field of view with very low optical distortion (<5% pin cushion or barrel distortion), compared to the typical <20% distortion. The optic will permit non-contact surface roughness measurements using optical techniques. This optic will permit simultaneous collection of selected image plane data, which data can then be subsequently optically processed. The image analysis will yield non-contact surface topology data for inspection where access to the surface does not permit a mechanical styles profilometer verification of surface topology. The optic allows a very broad spectral band or range of optical inspection. It is capable of spectroscopic imaging and fluorescence induced imaging when a scanning illumination source is used. The total viewing angle for this optic is 10 degrees for the full field of view of 10 degrees, compared to 40-70 degrees full angle field of view of the conventional gradient index or GRIN's lens systems.

  16. DOE Zero Energy Ready Home Case Study: Clifton View Homes, Coupeville, Washington

    SciTech Connect (OSTI)

    none,

    2013-09-01

    Ted Clifton, founder of Clifton View Homes, achieved an impressive Home Energy Rating System (HERS) score of 34 (without solar panels) on a two-story home completed in July 2011 that also earned him his first Challenge Home certification from the U.S. Department of Energy (DOE). This home also garnered a 2013 Housing Innovation Award in the "systems builder" category.

  17. Evolutionary analyses of non-family genes in plants

    SciTech Connect (OSTI)

    Ye, Chuyu; Li, Ting; Yin, Hengfu; Weston, David; Tuskan, Gerald A; Tschaplinski, Timothy J; Yang, Xiaohan

    2013-03-01

    There are a large number of non-family (NF) genes that do not cluster into families with three or more members per genome. While gene families have been extensively studied, a systematic analysis of NF genes has not been reported. We performed comparative studies on NF genes in 14 plant species. Based on the clustering of protein sequences, we identified ~94,000 NF genes across these species that were divided into five evolutionary groups: Viridiplantae-wide, angiosperm-specific, monocot-specific, dicot-specific, and those that were species-specific. Our analysis revealed that the NF genes resulted largely from less frequent gene duplications and/or a higher rate of gene loss after segmental duplication relative to genes in both low-copy-number families (LF; 3 10 copies per genome) and high-copy-number families (HF; >10 copies). Furthermore, we identified functions enriched in the NF gene set as compared with the HF genes. We found that NF genes were involved in essential biological processes shared by all plant lineages (e.g., photosynthesis and translation), as well as gene regulation and stress responses associated with phylogenetic diversification. In particular, our analysis of an Arabidopsis protein-protein interaction network revealed that hub proteins with the top 10% most connections were over-represented in the NF set relative to the HF set. This research highlights the roles that NF genes may play in evolutionary and functional genomics research.

  18. Evolutionary analyses of non-family genes in plants

    SciTech Connect (OSTI)

    Ye, Chuyu; Li, Ting; Yin, Hengfu; Weston, David; Tuskan, Gerald A; Tschaplinski, Timothy J; Yang, Xiaohan

    2013-01-01

    There are a large number of non-family (NF) genes that do not cluster into families with three or more members per genome. While gene families have been extensively studied, a systematic analysis of NF genes has not been reported. We performed comparative studies on NF genes in 14 plant species. Based on the clustering of protein sequences, we identified ~94 000 NF genes across these species that were divided into five evolutionary groups: Viridiplantae wide, angiosperm specific, monocot specific, dicot specific, and those that were species specific. Our analysis revealed that the NF genes resulted largely from less frequent gene duplications and/or a higher rate of gene loss after segmental duplication relative to genes in both lowcopy- number families (LF; 3 10 copies per genome) and high-copy-number families (HF; >10 copies). Furthermore, we identified functions enriched in the NF gene set as compared with the HF genes. We found that NF genes were involved in essential biological processes shared by all plant lineages (e.g. photosynthesis and translation), as well as gene regulation and stress responses associated with phylogenetic diversification. In particular, our analysis of an Arabidopsis protein protein interaction network revealed that hub proteins with the top 10% most connections were over-represented in the NF set relative to the HF set. This research highlights the roles that NF genes may play in evolutionary and functional genomics research.

  19. Mechanisms of radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.

    1996-10-01

    In the process of identifying genes differentially expressed in cells exposed ultraviolet radiation, we have identified a transcript having a 26-bp region that is highly conserved in a variety of species including Bacillus circulans, yeast, pumpkin, Drosophila, mouse, and man. When the 5` region (flanking region or UTR) of a gene, the sequence is predominantly in +/+ orientation with respect to the coding DNA strand; while in the coding region and the 3` region (UTR), the sequence is most frequently in the +/-orientation with respect to the coding DNA strand. In two genes, the element is split into two parts; however, in most cases, it is found only once but with a minimum of 11 consecutive nucleotides precisely depicting the original sequence. The element is found in a large number of different genes with diverse functions (from human ras p21 to B. circulans chitonase). Gel shift assays demonstrated the presence of a protein in HeLa cell extracts that binds to the sense and antisense single-stranded consensus oligomers, as well as to the double- stranded oligonucleotide. When double-stranded oligomer was used, the size shift demonstrated as additional protein-oligomer complex larger than the one bound to either sense or antisense single-stranded consensus oligomers alone. It is speculated either that this element binds to protein(s) important in maintaining DNA is a single-stranded orientation for transcription or, alternatively that this element is important in the transcription-coupled DNA repair process.

  20. Gene encoding acetyl-coenzyme A carboxylase

    DOE Patents [OSTI]

    Roessler, P.G.; Ohlrogge, J.B.

    1996-09-24

    A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives are disclosed which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides. 5 figs.

  1. Current trends in mapping human genes

    SciTech Connect (OSTI)

    Mckusick, V.A. )

    1991-01-01

    The human is estimated to have at least 50,000 expressed genes (gene loci). Some information is available concerning about 5,000 of these gene loci and about 1,900 have been mapped, i.e., assigned to specific chromosomes (and in most instances particular chromosome regions). Progress has been achieved by a combination of physical mapping (e.g., study of somatic cell hybrids and chromosomal in situ hybridization) and genetic mapping (e.g., genetic linkage studies). New methods for both physical and genetic mapping are expanding the armamentarium. The usefulness of the mapping information is already evident; the spin-off from the Human Genome Project (HGP) begins immediately. the complete nucleotide sequence is the ultimate map of the human genome. Sequencing, although already under way for limited segments of the genome, will await further progress in gene mapping, and in particular creation of contig maps for each chromosome. Meanwhile the technology of sequencing and sequence information handling will be developed.

  2. Transcriptional enhancer from milk protein genes

    DOE Patents [OSTI]

    Casperson, Gerald F.; Schmidhauser, Christian T.; Bissell, Mina J.

    1999-01-01

    The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

  3. Gene encoding acetyl-coenzyme A carboxylase

    DOE Patents [OSTI]

    Roessler, Paul G. (Golden, CO); Ohlrogge, John B. (Okemos, MI)

    1996-01-01

    A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.

  4. Celebrating Earth Day every day | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (Journal Article) | SciTech Connect Cationic phospholipids: structure transfection activity relationships Citation Details In-Document Search Title: Cationic phospholipids: structure transfection activity relationships Synthetic cationic lipids are presently the most widely used non-viral gene carriers. Examined here is a particularly attractive cationic lipid class, triester phosphatidylcholines (PCs) exhibiting low toxicities and good transfection efficiency. Similarly to other cationic

  5. Global prevalence and distribution of genes and microorganisms involved in mercury methylation

    SciTech Connect (OSTI)

    Podar, Mircea; Gilmour, C C; Brandt, Craig C; Bullock, Allyson L; Brown, Steven D; Crable, Bryan R; Palumbo, Anthony Vito; Somenahally, Anil C; Elias, Dwayne A

    2015-01-01

    Mercury methylation produces the neurotoxic, highly bioaccumulative methylmercury (MeHg). Recent identification of the methylation genes (hgcAB) provides the foundation for broadly evaluating microbial Hg-methylation potential in nature without making explicit rate measurements. We queried hgcAB diversity and distribution in all available microbial metagenomes, encompassing most environments. The genes were found in nearly all anaerobic, but not in aerobic, environments including oxygenated layers of the open ocean. Critically, hgcAB was effectively absent in ~1500 human microbiomes, suggesting a low risk of endogenous MeHg production. New potential methylation habitats were identified, including invertebrate guts, thawing permafrost, coastal dead zones , soils, sediments, and extreme environments, suggesting multiple routes for MeHg entry into food webs. Several new taxonomic groups potentially capable of Hg-methylation emerged, including lineages having no cultured representatives. We begin to address long-standing evolutionary questions about Hg-methylation and ancient carbon fixation mechanisms while generating a new global view of Hg-methylation potential.

  6. Global prevalence and distribution of genes and microorganisms involved in mercury methylation

    SciTech Connect (OSTI)

    Podar, Mircea; Gilmour, C. C.; Brandt, Craig C.; Soren, Allyson; Brown, Steven D.; Crable, Bryan R.; Palumbo, Anthony Vito; Somenahally, Anil C.; Elias, Dwayne A.

    2015-01-01

    Mercury methylation produces the neurotoxic, highly bioaccumulative methylmercury (MeHg). Recent identification of the methylation genes (hgcAB) provides the foundation for broadly evaluating microbial Hg-methylation potential in nature without making explicit rate measurements. We first queried hgcAB diversity and distribution in all available microbial metagenomes, encompassing most environments. The genes were found in nearly all anaerobic, but not in aerobic, environments including oxygenated layers of the open ocean. Critically, hgcAB was effectively absent in ~1500 human microbiomes, suggesting a low risk of endogenous MeHg production. New potential methylation habitats were identified, including invertebrate guts, thawing permafrost, coastal dead zones, soils, sediments, and extreme environments, suggesting multiple routes for MeHg entry into food webs. Several new taxonomic groups potentially capable of Hg-methylation emerged, including lineages having no cultured representatives. We then begin to address long-standing evolutionary questions about Hg-methylation and ancient carbon fixation mechanisms while generating a new global view of Hg-methylation potential.

  7. Visualization and Analysis of 3D Gene Expression Data (Technical...

    Office of Scientific and Technical Information (OSTI)

    Technical Report: Visualization and Analysis of 3D Gene Expression Data Citation Details In-Document Search Title: Visualization and Analysis of 3D Gene Expression Data You are...

  8. Modulation of histone methylation and MLH1 gene silencing by...

    Office of Scientific and Technical Information (OSTI)

    ... Subject: 60 APPLIED LIFE SCIENCES; ARGININE; CARCINOGENS; CHROMIUM; DNA DAMAGES; GENES; HUMAN POPULATIONS; LUNGS; LYSINE; METHYLATION; NEOPLASMS; OCCUPATIONAL EXPOSURE; PROMOTERS ...

  9. QB1 - Stochastic Gene Regulation Munsky, Brian [Los Alamos National

    Office of Scientific and Technical Information (OSTI)

    FACILITIES; FLUCTUATIONS; GENE REGULATION; KINETICS; QUANTIZATION; SIMULATION; SWITCHES Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present...

  10. Ataxia-telangiectasia: mutations in the ATM gene

    DOE Patents [OSTI]

    Gatti, Richard A.; Concannon, Patrick J.

    1999-01-01

    The invention is related to ataxia-telangiectasia, specifically, mutations in the ataxia-telangiectasia mutated gene.

  11. Listening to the noise: random fluctuations reveal gene network...

    Office of Scientific and Technical Information (OSTI)

    frequently manifests as significant phenotypic differences within the cellular population. ... Subject: 59 BASIC BIOLOGICAL SCIENCES; EXCITATION; FLUCTUATIONS; GENES; GENETICS; ORIGIN; ...

  12. Expression of the tumor suppressor gene, p53, during the development...

    Office of Scientific and Technical Information (OSTI)

    stages and probed for altered expression of oncogenes and tumor suppressor genes. ... GENE REGULATION; MESSENGER-RNA; MICE; ONCOGENES; ANIMALS; GENES; MAMMALS; MUTATIONS; ...

  13. March 2016 Most Viewed Documents for Biology And Medicine | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information March 2016 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. [Brookhaven National Lab., Upton, NY (United States)]|[State Univ. of New York at Stony Brook, Stony Brook, NY (United States). Dept. of Psychiatry]; Fowler, J.S.; Logan, J.; Wang, G.J. [Brookhaven National Lab., Upton, NY (United States)] (1995) 206 Drug Retention Times Center for Human Reliability Studies

  14. March 2016 Most Viewed Documents for Geosciences | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information March 2016 Most Viewed Documents for Geosciences Gas-phase diffusion in porous media: Evaluation of an advective- dispersive formulation and the dusty-gas model including comparison to data for binary mixtures Webb, S.W. (1996) 88 Evaluation of soil liquefaction potential for level ground during earthquakes. A summary report Seed, H.B.; Arango, I.; Chan, C.K. (1975) 71 Fundamental Research on Percussion Drilling: Improved rock mechanics

  15. March 2016 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information March 2016 Most Viewed Documents for Renewable Energy Sources Wind energy applications guide anon. (2001) 557 Aerodynamic characteristics of seven symmetrical airfoil sections through 180-degree angle of attack for use in aerodynamic analysis of vertical axis wind turbines Sheldahl, R E; Klimas, P C (1981) 474 Calculation of brine properties. [Above 80/sup 0/F and for salt content between 5 and 25%] Dittman, G.L. (1977) 285

  16. April 2013 Most Viewed Documents for Biology And Medicine | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Biology And Medicine Science Subject Feed Publications in biomedical and environmental sciences programs, 1981 Moody, J.B. (comp.) (1982) 306 /> Drug Retention Times Center for Human Reliability Studies (2007) 99 /> SURVEY OF NOISE SUPPRESSION SYSTEMS FOR ENGINE GENERATOR SETS. KRISHNA,C.R. (1999) 95 /> Defining the Effectiveness of UV Lamps Installed in Circulating Air Ductwork Douglas

  17. April 2013 Most Viewed Documents for Environmental Sciences | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Environmental Sciences Science Subject Feed Building a secondary containment system Broder, M.F. (1994) 144 /> Methods and opportunities in the recycling of rare earth based materials Ellis, T.W.; Schmidt, F.A.; Jones, L.L. (1994) 134 /> Ecological Screening Values for Surface Water, Sediment, and Soil Friday, G. P. (1999) 128 /> Ammonia usage in vapor compression for refrigeration and

  18. April 2013 Most Viewed Documents for Geosciences | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information April 2013 Most Viewed Documents for Geosciences Sloshing analysis of viscous liquid storage tanks Uras, R.Z. (1995) 83 Seismic design evaluation guidelines for buried piping for the DOE HLW Facilities Lin, Chi-Wen [Consultant, Martinez, CA (United States)]; Antaki, G. [Westinghouse Savannah River Co., Aiken, SC (United States)]; Bandyopadhyay, K. [Brookhaven National Lab., Upton, NY (United States)]; Bush, S.H. [Review & Synthesis

  19. April 2013 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for Mathematics And Computing Science Subject Feed Publications in biomedical and environmental sciences programs, 1981 Moody, J.B. (comp.) (1982) 306 /> A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 159 /> Lecture notes for introduction to safety and health Biele, F. (1992) 138 /> Analytical considerations in the code qualification of

  20. April 2013 Most Viewed Documents for Physics | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information April 2013 Most Viewed Documents for Physics Science Subject Feed Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; Chou, B.E. (1978) 123 /> Plastic Gamma Sensors: An Application in Detection of Radioisotopes S. Mukhopadhyay (2003) 85 /> Organic light emitting diodes (OLEDS) and OLED-based structurally integrated optical sensors Cai, Yuankun (2010) 83 /> White LED with High Package

  1. SCIENCE ON SATURDAY- "A Robot's View of Our Ocean Planet" | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab 16, 2013, 9:30am Science On Saturday MBG Auditorium SCIENCE ON SATURDAY- "A Robot's View of Our Ocean Planet" Professor Josh Kohut, Assistant Professor of Oceanography Center for Coastal Physical Oceanography, Rutgers University Presentation: PDF icon SOS16MAR2013_JKohut.pdf Science on Saturday is a series of lectures given by scientists, mathematicians, and other professionals involved in cutting-edge research. Held on Saturday mornings throughout winter, the

  2. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  3. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  4. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  5. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  6. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  7. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  8. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  9. Residential Windows and Window Coverings: A Detailed View of the Installed Base and User Behavior

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Residential Windows and Window Coverings: A Detailed View of the Installed Base and User Behavior SEPTEMBER 2013 Prepared for: Building Technologies Office Office of Energy Efficiency and Renewable Energy U.S. Department of Energy Prepared by: D&R International, Ltd. September 2013 Prepared for: Building Technologies Office Office of Energy Efficiency and Renewable Energy U.S. Department of Energy Prepared By: D&R International, Ltd. 1300 Spring Street, Suite 500 Silver Spring, MD 20910

  10. 3D View Inside the Skeleton with X-ray Microscopy: Imaging Bone at the

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nanoscale 3D View Inside the Skeleton with X-ray Microscopy: Imaging Bone at the Nanoscale Scientists studying osteoporosis and other skeletal diseases are interested in the 3D structure of bone and its responses to conditions such as weightlessness, radiation (of particular interest to astronauts) and vitamin D deficiency. The current gold standard, micro-computed tomography (micro-CT), provides 3D images of trabeculae, the small interior struts of bone tissue, and electron microscopy can

  11. September 2013 Most Viewed Documents for Biology And Medicine | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for Biology And Medicine Science Subject Feed Drug Retention Times Center for Human Reliability Studies (2007) 29 /> Oleoresin Capsicum toxicology evaluation and hazard review Archuleta, M.M. (1995) 27 /> SURVEY OF NOISE SUPPRESSION SYSTEMS FOR ENGINE GENERATOR SETS. KRISHNA,C.R. (1999) 27 /> Site-Directed Research and Development FY 2012 Annual Report , (2013) 27 /> Human

  12. September 2015 Most Viewed Documents for Biology And Medicine | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. [Brookhaven National Lab., Upton, NY (United States)]|[State Univ. of New York at Stony Brook, Stony Brook, NY (United States). Dept. of Psychiatry]; Fowler, J.S.; Logan, J.; Wang, G.J. [Brookhaven National Lab., Upton, NY (United States)] (1995) 168 Drug Retention Times Center for Human Reliability

  13. January 2013 Most Viewed Documents for Biology And Medicine | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Biology And Medicine Nevada Test Site Radiological Control Manual Radiological Control Managers' Council Nevada Test Site Effects of ionizing radiation on the boreal forest: Canada's FIG experiment, with implications for radionuclides Amiro, B.D.; Sheppard, S.C Assessment of atmospheric metallic pollution in the metropolitan region of Sao Paulo, Brazil, employing Tillandsia usneoides L. as

  14. January 2013 Most Viewed Documents for Materials | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information January 2013 Most Viewed Documents for Materials Evaluation of the inhibitive effect of some plant extracts on the acid corrosion of mild steel Oguzie, Emeka E. [Electrochemistry and Materials Science Research Laboratory, Department of Chemistry, Federal University of Technology, PMB 1526, Owerri (Nigeria)], E-mail: oguziemeka@yahoo.com Superconducting wind turbine generators Abrahamsen, A B; Seiler, E; Zirngibl, T; Andersen, N H [Materials

  15. January 2013 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Mathematics And Computing Cybersecurity through Real-Time Distributed Control Systems Kisner, Roger A [ORNL]; Manges, Wayne W [ORNL]; MacIntyre, Lawrence Paul [ORNL]; Nutaro, James J [ORNL]; Munro Jr, John K [ORNL]; Ewing, Paul D [ORNL]; Howlader, Mostofa [ORNL]; Kuruganti, Phani Teja [ORNL]; Wallace, Richard M [ORNL]; Olama, Mohammed M [ORNL] REACTOR ANALYSIS AND VIRTUAL CONTROL ENVIRONMENT

  16. January 2013 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for Renewable Energy Sources Photovoltaic Materials Duty, C.; Angelini, J.; Armstrong, B.; Bennett, C.; Evans, B.; Jellison, G. E.; Joshi, P.; List, F.; Paranthaman, P.; Parish, C.; Wereszczak, A. High Efficiency CdTe and CIGS Thin Film Solar Cells: Highlights of the Technologies Challenges (Presentation) Noufi, R. Accelerated UV Test Methods for Encapsulants of Photovoltaic Modules

  17. July 2013 Most Viewed Documents for Biology And Medicine | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Biology And Medicine Science Subject Feed Carbon Dioxide Sequestering Using Microalgal Systems Daniel J. Stepan; Richard E. Shockey; Thomas A. Moe; Ryan Dorn (2002) 51 /> SURVEY OF NOISE SUPPRESSION SYSTEMS FOR ENGINE GENERATOR SETS. KRISHNA,C.R. (1999) 46 /> Human radiation studies: Remembering the early years. Oral history of Donner Lab Administrator Baird G. Whaley, August 15, 1994 NONE (1995)

  18. July 2013 Most Viewed Documents for Engineering | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information July 2013 Most Viewed Documents for Engineering Science Subject Feed Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 513 /> Charpy impact test results on five materials and NIST verification specimens using instrumented 2-mm and 8-mm strikers Nanstad, R.K.; Sokolov, M.A. (1995) 241 /> Conduction heat transfer solutions VanSant, J.H. (1980) 194 /> Damage identification and health monitoring of

  19. July 2013 Most Viewed Documents for Environmental Sciences | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Environmental Sciences Science Subject Feed Building a secondary containment system Broder, M.F. (1994) 98 /> Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 76 /> Ammonia usage in vapor compression for refrigeration and air-conditioning in the United States Fairchild, P.D.; Baxter, V.D. (1995) 58 /> Mitigation options for

  20. July 2013 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information July 2013 Most Viewed Documents for Mathematics And Computing Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 567 /> A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 89 /> Lecture notes for introduction to safety and health Biele, F. (1992) 78 /> Computational procedures for determining parameters

  1. June 2014 Most Viewed Documents for Biology And Medicine | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Biology And Medicine Science Subject Feed Modification to the Monte Carlo N-Particle (MCNP) Visual Editor (MCNPVised) to Read in Computer Aided Design (CAD) Files Randolph Schwarz; Leland L. Carter; Alysia Schwarz (2005) 37 /> Geothermal demonstration: Zunil food dehydration facility Maldonado, O. (Consultecnia, Guatemala City (Guatemala)); Altseimer, J.; Thayer, G.R. (Los Alamos National Lab., NM

  2. June 2014 Most Viewed Documents for Environmental Sciences | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Environmental Sciences Science Subject Feed Proceedings of the fifth annual NEA-seabed working group meeting Anderson, D. R. [ed.] (1980) 63 /> Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 50 /> Statistical methods for environmental pollution monitoring Gilbert, R.O. (1987) 42 /> Land use and energy Robeck, K.E.; Ballou,

  3. June 2014 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for Mathematics And Computing Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 337 /> The Effects of Nuclear Weapons Glasstone, Samuel (1964) 71 /> Levenberg--Marquardt algorithm: implementation and theory More, J.J. (1977) 68 /> Computational procedures for determining parameters in Ramberg-Osgood elastoplastic

  4. June 2014 Most Viewed Documents for National Defense | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information June 2014 Most Viewed Documents for National Defense Science Subject Feed The Effects of Nuclear Weapons Glasstone, Samuel (1964) 71 /> Penetration equations Young, C.W. [Applied Research Associates, Inc., Albuquerque, NM (United States)] (1997) 29 /> Manual for the prediction of blast and fragment loadings on structures Not Available (1980) 29 /> Specific heat and thermal conductivity of explosives, mixtures, and plastic-bonded

  5. June 2015 Most Viewed Documents for Biology And Medicine | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. [Brookhaven National Lab., Upton, NY (United States)]|[State Univ. of New York at Stony Brook, Stony Brook, NY (United States). Dept. of Psychiatry]; Fowler, J.S.; Logan, J.; Wang, G.J. [Brookhaven National Lab., Upton, NY (United States)] (1995) 115 Dose and volume specification for reporting interstitial therapy

  6. June 2015 Most Viewed Documents for Environmental Sciences | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Environmental Sciences Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 234 Statistical methods for environmental pollution monitoring Gilbert, R.O. (1987) 102 Building a secondary containment system Broder, M.F. (1994) 97 Mitigation options for accidental releases of hazardous gases Fthenakis, V.M. (1995) 87 Ammonia usage in vapor

  7. June 2015 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Mathematics And Computing Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 833 Lecture notes for introduction to safety and health Biele, F. (1992) 256 Systems engineering management plans. Rodriguez, Tamara S. (2009) 218 A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 216 Ferrite

  8. March 2014 Most Viewed Documents for Biology And Medicine | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Biology And Medicine Science Subject Feed Carbon Dioxide Sequestering Using Microalgal Systems Daniel J. Stepan; Richard E. Shockey; Thomas A. Moe; Ryan Dorn (2002) 30 /> Dose and volume specification for reporting interstitial therapy NONE (1997) 29 /> Geothermal demonstration: Zunil food dehydration facility Maldonado, O. (Consultecnia, Guatemala City (Guatemala)); Altseimer, J.; Thayer, G.R. (Los

  9. March 2014 Most Viewed Documents for Environmental Sciences | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Environmental Sciences Science Subject Feed Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 57 /> Atmospheric Chemistry and Greenhouse Gases Ehhalt, D.; Prather, M.; Dentener, F.; Derwent, R.; Dlugokencky, Edward J.; Holland, E.; Isaksen, I.; Katima, J.; Kirchhoff, V.; Matson, P.; Midgley, P.; Wang, M.; Berntsen, T.; Bey, I.; Brasseur,

  10. March 2014 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information 4 Most Viewed Documents for Mathematics And Computing Science Subject Feed Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 291 /> Ten Problems in Experimental Mathematics Bailey, David H.; Borwein, Jonathan M.; Kapoor, Vishaal;Weisstein, Eric (2004) 101 /> The Effects of Nuclear Weapons Glasstone, Samuel (1964) 72 /> Levenberg--Marquardt algorithm: implementation and

  11. March 2015 Most Viewed Documents for Engineering | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information 5 Most Viewed Documents for Engineering Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 325 Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste] Rezvani, M.A.; Ziada, H.H. (Westinghouse Hanford Co., Richland, WA (United States)); Shurrab, M.S. (Westinghouse Savannah River Co., Aiken, SC (United States)) (1992) 223 Conduction heat transfer

  12. March 2015 Most Viewed Documents for Environmental Sciences | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Environmental Sciences Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) 229 Statistical methods for environmental pollution monitoring Gilbert, R.O. (1987) 131 Health and environmental effects document on geothermal energy: 1981 Layton, D.W.; Anspaugh, L.R.; O'Banion, K.D. (1981) 95 Study of using oxygen-enriched combustion air for

  13. March 2015 Most Viewed Documents for Mathematics And Computing | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Mathematics And Computing Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 1019 A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 183 Lecture notes for introduction to safety and health Biele, F. (1992) 172 Mort User's Manual: For use with the Management Oversight and Risk Tree analytical logic

  14. Most Viewed Documents for Biology and Medicine: December 2014 | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information Most Viewed Documents for Biology and Medicine: December 2014 Dose and volume specification for reporting interstitial therapy NONE (1997) 38 Modification to the Monte Carlo N-Particle (MCNP) Visual Editor (MCNPVised) to Read in Computer Aided Design (CAD) Files Randolph Schwarz; Leland L. Carter; Alysia Schwarz (2005) 38 Drug Retention Times Center for Human Reliability Studies (2007) 34 Degradation of high concentrations of

  15. Most Viewed Documents for National Defense: December 2014 | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information Most Viewed Documents for National Defense: December 2014 A survey of combustible metals, thermites, and intermetallics for pyrotechnic applications Fischer, S.H.; Grubelich, M.C. (1996) 53 Precision linear shaped charge analyses for severance of metals Vigil, M.G. (1996) 36 Comments on TNT Equivalence Cooper, P.W. (1994) 32 Specific heat and thermal conductivity of explosives, mixtures, and plastic-bonded explosives determined

  16. December 2015 Most Viewed Documents for Biology And Medicine | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. [Brookhaven National Lab., Upton, NY (United States)]|[State Univ. of New York at Stony Brook, Stony Brook, NY (United States). Dept. of Psychiatry]; Fowler, J.S.; Logan, J.; Wang, G.J. [Brookhaven National Lab., Upton, NY (United States)] (1995) 160 Drug Retention Times Center for Human Reliability

  17. December 2015 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Renewable Energy Sources Aerodynamic characteristics of seven symmetrical airfoil sections through 180-degree angle of attack for use in aerodynamic analysis of vertical axis wind turbines Sheldahl, R E; Klimas, P C (1981) 307 Calculation of brine properties. [Above 80/sup 0/F and for salt content between 5 and 25%] Dittman, G.L. (1977) 228 Temperature coefficients for PV modules and

  18. Going Back to Californium: A Changing View of Covalency | U.S. DOE Office

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Science (SC) Going Back to Californium: A Changing View of Covalency Basic Energy Sciences (BES) BES Home About Research Facilities Science Highlights Benefits of BES Funding Opportunities Basic Energy Sciences Advisory Committee (BESAC) Community Resources Contact Information Basic Energy Sciences U.S. Department of Energy SC-22/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3081 F: (301) 903-6594 E: Email Us More Information » 05.01.15 Going Back to

  19. A Network-based View of the U.S. Energy Sector

    U.S. Energy Information Administration (EIA) Indexed Site

    A Network-based View of the U.S. Energy Sector Vipin Arora | Elizabeth Sendich | Julia Teng February 2016 Independent Statistics & Analysis www.eia.gov U.S. Energy Information Administration Washington, DC 20585 This paper is released to encourage discussion and critical comment. The analysis and conclusions expressed here are those of the authors and not necessarily those of the U.S. Energy Information Administration. WORKING PAPER SERIES February 2016 Vipin Arora, Elizabeth Sendich, and

  20. Towards an informative mutant phenotype for every bacterial gene

    SciTech Connect (OSTI)

    Deutschbauer, Adam; Price, Morgan N.; Wetmore, Kelly M.; Tarjan, Daniel R.; Xu, Zhuchen; Shao, Wenjen; Leon, Dacia; Arkin, Adam P.; Skerker, Jeffrey M.

    2014-08-11

    Mutant phenotypes provide strong clues to the functions of the underlying genes and could allow annotation of the millions of sequenced yet uncharacterized bacterial genes. However, it is not known how many genes have a phenotype under laboratory conditions, how many phenotypes are biologically interpretable for predicting gene function, and what experimental conditions are optimal to maximize the number of genes with a phenotype. To address these issues, we measured the mutant fitness of 1,586 genes of the ethanol-producing bacterium Zymomonas mobilis ZM4 across 492 diverse experiments and found statistically significant phenotypes for 89% of all assayed genes. Thus, in Z. mobilis, most genes have a functional consequence under laboratory conditions. We demonstrate that 41% of Z. mobilis genes have both a strong phenotype and a similar fitness pattern (cofitness) to another gene, and are therefore good candidates for functional annotation using mutant fitness. Among 502 poorly characterized Z. mobilis genes, we identified a significant cofitness relationship for 174. For 57 of these genes without a specific functional annotation, we found additional evidence to support the biological significance of these gene-gene associations, and in 33 instances, we were able to predict specific physiological or biochemical roles for the poorly characterized genes. Last, we identified a set of 79 diverse mutant fitness experiments in Z. mobilis that are nearly as biologically informative as the entire set of 492 experiments. Therefore, our work provides a blueprint for the functional annotation of diverse bacteria using mutant fitness.

  1. Towards an informative mutant phenotype for every bacterial gene

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Deutschbauer, Adam; Price, Morgan N.; Wetmore, Kelly M.; Tarjan, Daniel R.; Xu, Zhuchen; Shao, Wenjen; Leon, Dacia; Arkin, Adam P.; Skerker, Jeffrey M.

    2014-08-11

    Mutant phenotypes provide strong clues to the functions of the underlying genes and could allow annotation of the millions of sequenced yet uncharacterized bacterial genes. However, it is not known how many genes have a phenotype under laboratory conditions, how many phenotypes are biologically interpretable for predicting gene function, and what experimental conditions are optimal to maximize the number of genes with a phenotype. To address these issues, we measured the mutant fitness of 1,586 genes of the ethanol-producing bacterium Zymomonas mobilis ZM4 across 492 diverse experiments and found statistically significant phenotypes for 89% of all assayed genes. Thus, inmore » Z. mobilis, most genes have a functional consequence under laboratory conditions. We demonstrate that 41% of Z. mobilis genes have both a strong phenotype and a similar fitness pattern (cofitness) to another gene, and are therefore good candidates for functional annotation using mutant fitness. Among 502 poorly characterized Z. mobilis genes, we identified a significant cofitness relationship for 174. For 57 of these genes without a specific functional annotation, we found additional evidence to support the biological significance of these gene-gene associations, and in 33 instances, we were able to predict specific physiological or biochemical roles for the poorly characterized genes. Last, we identified a set of 79 diverse mutant fitness experiments in Z. mobilis that are nearly as biologically informative as the entire set of 492 experiments. Therefore, our work provides a blueprint for the functional annotation of diverse bacteria using mutant fitness.« less

  2. RNA polymerase gene, microorganism having said gene and the production of RNA polymerase by the use of said microorganism

    DOE Patents [OSTI]

    Kotani, Hirokazu; Hiraoka, Nobutsugu; Obayashi, Akira

    1991-01-01

    SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.

  3. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... 17. By combining the data from this chromosome-mediated gene transfer transfectant panel, conventional somatic-cell hybrids containing well-defined breaks on chromosome 17, and ...

  4. Gene Calling Standards (GSC8 Meeting)

    ScienceCinema (OSTI)

    Kyrpides, Nikos [Genome Biology Program, DOE JGI

    2011-04-28

    The Genomic Standards Consortium was formed in September 2005. It is an international, open-membership working body which promotes standardization in the description of genomes and the exchange and integration of genomic data. The 2009 meeting was an activity of a five-year funding "Research Coordination Network" from the National Science Foundation and was organized held at the DOE Joint Genome Institute with organizational support provided by the JGI and by the University of California - San Diego. Nikos Kyrpides of the DOE Joint Genome Institute discusses gene calling standards at the Genomic Standards Consortium's 8th meeting at the DOE JGI in Walnut Creek, Calif. on Sept. 10, 2009.

  5. Few-view cone-beam CT reconstruction with deformed prior image

    SciTech Connect (OSTI)

    Zhang, Hua; Ouyang, Luo; Wang, Jing E-mail: jing.wang@utsouthwestern.edu; Huang, Jing; Ma, Jianhua E-mail: jing.wang@utsouthwestern.edu; Chen, Wufan

    2014-12-15

    Purpose: Prior images can be incorporated into the image reconstruction process to improve the quality of subsequent cone-beam CT (CBCT) images from sparse-view or low-dose projections. The purpose of this work is to develop a deformed prior image-based reconstruction (DPIR) strategy to mitigate the deformation between the prior image and the target image. Methods: The deformed prior image is obtained by a projection-based registration approach. Specifically, the deformation vector fields used to deform the prior image are estimated through iteratively matching the forward projection of the deformed prior image and the measured on-treatment projections. The deformed prior image is then used as the prior image in the standard prior image constrained compressed sensing (PICCS) algorithm. A simulation study on an XCAT phantom and a clinical study on a head-and-neck cancer patient were conducted to evaluate the performance of the proposed DPIR strategy. Results: The deformed prior image matches the geometry of the on-treatment CBCT more closely as compared to the original prior image. Consequently, the performance of the DPIR strategy from few-view projections is improved in comparison to the standard PICCS algorithm, based on both visual inspection and quantitative measures. In the XCAT phantom study using 20 projections, the average root mean squared error is reduced from 14% in PICCS to 10% in DPIR, and the average universal quality index increases from 0.88 in PICCS to 0.92 in DPIR. Conclusions: The present DPIR approach provides a practical solution to the mismatch problem between the prior image and target image, which improves the performance of the original PICCS algorithm for CBCT reconstruction from few-view or low-dose projections.

  6. March 2016 Most Viewed Documents for Chemistry | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information March 2016 Most Viewed Documents for Chemistry LITERATURE REVIEW OF BORIC ACID SOLUBILITY DATA Crapse, K.; Kyser, E. (2011) 342 Electrical conductivity measurements of aqueous electrolyte solutions at high temperatures and high pressures Ho, P.C.; Palmer, D.A. (1995) 331 Decomposition of calcium sulfate: a review of the literature. [62 refs] Swift, W M; Panek, A F; Smith, G W; Vogel, G J; Jonke, A A (1976) 175 Vapor-liquid equilibria for nitric

  7. March 2016 Most Viewed Documents for National Defense | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information March 2016 Most Viewed Documents for National Defense SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 201 Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 143 Comments on TNT Equivalence Cooper, P.W. (1994) 135 LLNL small-scale drop-hammer impact sensitivity test

  8. April 2013 Most Viewed Documents for Chemistry | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information April 2013 Most Viewed Documents for Chemistry Science Subject Feed Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; Chou, B.E. (1978) 123 /> Electrical conductivity measurements of aqueous electrolyte solutions at high temperatures and high pressures Ho, P.C.; Palmer, D.A. (1995) 102 /> Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions

  9. April 2013 Most Viewed Documents for Materials | OSTI, US Dept of Energy,

    Office of Scientific and Technical Information (OSTI)

    Office of Scientific and Technical Information April 2013 Most Viewed Documents for Materials Science Subject Feed Fatigue design curves for 6061-T6 aluminum Yahr, G.T. (1993) 554 /> Charpy impact test results on five materials and NIST verification specimens using instrumented 2-mm and 8-mm strikers Nanstad, R.K.; Sokolov, M.A. (1995) 534 /> Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 488 /> The influence of grain size on the mechanical

  10. April 2013 Most Viewed Documents for National Defense | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information April 2013 Most Viewed Documents for National Defense Science Subject Feed Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 95 /> SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 69 /> LLNL small-scale drop-hammer impact sensitivity test Simpson, L.R.; Foltz,

  11. PPPL's "Star Power" video offers compelling view of Laboratory's

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    research | Princeton Plasma Physics Lab PPPL's "Star Power" video offers compelling view of Laboratory's research By Jeanne Jackson DeVoe September 4, 2013 Tweet Widget Google Plus One Share on Facebook PPPL has released its new "Star Power" informational video. (Photo by Greg Czechowicz/PPPL Office of Communications ) PPPL has released its new "Star Power" informational video. (Watch video: http://www.pppl.gov/star%20power) The U.S. Department of Energy's

  12. DOE Zero Energy Ready Home Case Study: Clifton View Homes, Coupeville, WA,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Systems Home | Department of Energy Coupeville, WA, Systems Home DOE Zero Energy Ready Home Case Study: Clifton View Homes, Coupeville, WA, Systems Home Case study of a DOE Zero Energy Ready Home on Whidbey Island, WA, that scored HERS 45 without PV. This 2,908-square-foot custom/system home has a SIP roof and walls, R-20 rigid foam under slab, triple-pane windows, ground source heat pump for radiant floor heat, and a unique balanced ventilation system using separate exhaust fans to bring

  13. DOE Zero Energy Ready Home Case Study: Clifton View Homes, Marine Drive and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Port Hadlcok, Coupeville and Port Hadlock WA | Department of Energy Marine Drive and Port Hadlcok, Coupeville and Port Hadlock WA DOE Zero Energy Ready Home Case Study: Clifton View Homes, Marine Drive and Port Hadlcok, Coupeville and Port Hadlock WA Case study of two DOE 2015 Housing Innovation Award winning custom homes in the marine climate that got HERS 39 and 38 without PV or HERS 2-12 and -9 with PV, with 6.5" SIP walls and 10.25" SIP roof; 11.75 ICF around slab, R-20 rigid

  14. DOE Zero Energy Ready Home Case Study: Clifton View Homes, Whidbey Island,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    WA | Department of Energy Whidbey Island, WA DOE Zero Energy Ready Home Case Study: Clifton View Homes, Whidbey Island, WA Case study of a DOE Zero Energy Ready home on Whidbey Island, WA, that scores HERS 37 without PV or HERS -13 with 10 kW PV, enough to power the home and an electric car. The two-story custom home has ICF below-grade walls, 6.5-inch SIP above-grade walls, a 10.25-in. SIP roof, and triple-pane windows. The home has a ground-source heat pump provides radiant floor heat

  15. Biomass stakeholder views and concerns: Environmental groups and some trade association

    SciTech Connect (OSTI)

    Peelle, E.

    2000-01-01

    This exploratory study of the views and concerns of 25 environmental organizations found high interest and concern about which biomass feedstocks would be used and how these biomass materials would be converted to energy. While all favored renewable energy over fossil or nuclear energy, opinion diverged over whether energy crops, residues, or both should be the primary source of a biomass/bioenergy fuel cycle. About half of the discussants favored biomass ``in general'' as a renewable energy source, while the others were distributed about equally over five categories, from favor-with-conditions, uncertain, skeptical, opposed, to ``no organizational policy.''

  16. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    SciTech Connect (OSTI)

    Hoen, Ben; Wiser, Ryan; Cappers, Peter; Thayer, Mark; Sethi, Gautam

    2010-04-01

    With an increasing number of communities considering nearby wind power developments, there is a need to empirically investigate community concerns about wind project development. One such concern is that property values may be adversely affected by wind energy facilities, and relatively little research exists on the subject. The present research investigates roughly 7,500 sales of single-family homes surrounding 24 existing U.S. wind facilities. Across four different hedonic models the results are consistent: neither the view of the wind facilities nor the distance of the home to those facilities is found to have a statistically significant effect on home sales prices.

  17. Consumer Views on Plug-in Electric Vehicles … National Benchmark Report

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Consumer Views on Plug-in Electric Vehicles - National Benchmark Report Mark Singer National Renewable Energy Laboratory Technical Report NREL/TP-5400-65279 January 2016 NREL is a national laboratory of the U.S. Department of Energy Office of Energy Efficiency & Renewable Energy Operated by the Alliance for Sustainable Energy, LLC This report is available at no cost from the National Renewable Energy Laboratory (NREL) at www.nrel.gov/publications. Contract No. DE-AC36-08GO28308 National

  18. September 2013 Most Viewed Documents for National Defense | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information September 2013 Most Viewed Documents for National Defense Science Subject Feed SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 34 /> Left-Wing Extremism: The Current Threat Karl A. Seger (2001) 28 /> The Elusive Coefficients of Thermal Expansion in PBX 9502 C.B. Skidmore; T.A. Butler; C.W. Sandoval (2003) 28 />

  19. September 2015 Most Viewed Documents for National Defense | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information September 2015 Most Viewed Documents for National Defense SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 131 Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 91 Smart bridge: a tool for estimating the military load classification of bridges Van Groningen, C.N.;

  20. January 2013 Most Viewed Documents for National Defense | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information January 2013 Most Viewed Documents for National Defense Dynamic equation of state and strength properties of unreacted PBXW-128 explosive Chhabildas, L.C.; Grady, D.E.; Reinhart, W.D.; Wilson, L.T. From separations to reconstitution - a short history of Plutonium in the U.S. and Russia Gray, L W Condensation induced water hammer safety Gintner, M.A. Direct calibration of the yield of nuclear explosion Nakanishi, K.; Nikolayev, A.

  1. June 2015 Most Viewed Documents for Renewable Energy Sources | OSTI, US

    Office of Scientific and Technical Information (OSTI)

    Dept of Energy, Office of Scientific and Technical Information June 2015 Most Viewed Documents for Renewable Energy Sources Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 240 Aerodynamic characteristics of seven symmetrical airfoil sections through 180-degree angle of attack for use in aerodynamic analysis of vertical axis wind turbines Sheldahl, R E; Klimas, P C (1981) 192 Calculation of brine properties. [Above 80/sup 0/F and for salt content between 5 and 25%]

  2. March 2015 Most Viewed Documents for Biology And Medicine | OSTI, US Dept

    Office of Scientific and Technical Information (OSTI)

    of Energy, Office of Scientific and Technical Information 5 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. [Brookhaven National Lab., Upton, NY (United States)]|[State Univ. of New York at Stony Brook, Stony Brook, NY (United States). Dept. of Psychiatry]; Fowler, J.S.; Logan, J.; Wang, G.J. [Brookhaven National Lab., Upton, NY (United States)] (1995) 134 Drug Retention Times Center for Human Reliability Studies (2007) 114

  3. December 2015 Most Viewed Documents for Engineering | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for Engineering Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 776 Charpy impact test results on five materials and NIST verification specimens using instrumented 2-mm and 8-mm strikers Nanstad, R.K.; Sokolov, M.A. (1995) 546 Conduction heat transfer solutions VanSant, J.H. (1980) 527 Automotive vehicle sensors Sheen, S.H.; Raptis, A.C.; Moscynski, M.J. (1995)

  4. December 2015 Most Viewed Documents for National Defense | OSTI, US Dept of

    Office of Scientific and Technical Information (OSTI)

    Energy, Office of Scientific and Technical Information December 2015 Most Viewed Documents for National Defense SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 113 Comments on TNT Equivalence Cooper, P.W. (1994) 98 Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 82 The future of components for high reliability

  5. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    SciTech Connect (OSTI)

    San Diego State University; Bard Center for Environmental Policy at Bard College; Hoen, Ben; Wiser, Ryan; Cappers, Peter; Thayer, Mark; Sethi, Gautam

    2011-06-23

    With increasing numbers of communities considering wind power developments, empirical investigations regarding related community concerns are needed. One such concern is that proximate property values may be adversely affected, yet relatively little research exists on the subject. The present research investigates roughly 7,500 sales of single-family homes surrounding 24 existing U.S. wind facilities. Across four different hedonic models, and a variety of robustness tests, the results are consistent: neither the view of the wind facilities nor the distance of the home to those facilities is found to have a statistically significant effect on sales prices, yet further research is warranted.

  6. New Forces at Work in Mining: Industry View of Critical Technologies

    SciTech Connect (OSTI)

    Peterson, D. J.; LaTourrette, Tom; Bartis, James T.

    2007-04-01

    RAND has just published a report entitled, "New Forces at Work in Mining: Industry Views of Critical Technologies," by D. J. Peterson, Tom LaTourrette, and James T. Bartis. The report presents the results of a series of in-depth discussions with leading mining industry representatives selected for their prominent position and their ability to think broadly about technology trends. The discussions highlighted the importance of collaborative technology research, development, and implementation strategies and the increasingly critical role of mine personnel in the utilization of new technologies.

  7. Gene set analyses for interpreting microarray experiments on prokaryotic organisms.

    SciTech Connect (OSTI)

    Tintle, Nathan; Best, Aaron; Dejongh, Matthew; VanBruggen, Dirk; Heffron, Fred; Porwollik, Steffen; Taylor, Ronald C.

    2008-11-05

    Background: Recent advances in microarray technology have brought with them the need for enhanced methods of biologically interpreting gene expression data. Recently, methods like Gene Set Enrichment Analysis (GSEA) and variants of Fisher’s exact test have been proposed which utilize a priori biological information. Typically, these methods are demonstrated with a priori biological information from the Gene Ontology. Results: Alternative gene set definitions are presented based on gene sets inferred from the SEED: open-source software environment for comparative genome annotation and analysis of microbial organisms. Many of these gene sets are then shown to provide consistent expression across a series of experiments involving Salmonella Typhimurium. Implementation of the gene sets in an analysis of microarray data is then presented for the Salmonella Typhimurium data. Conclusions: SEED inferred gene sets can be naturally defined based on subsystems in the SEED. The consistent expression values of these SEED inferred gene sets suggest their utility for statistical analyses of gene expression data based on a priori biological information

  8. Metagenomic gene annotation by a homology-independent approach

    SciTech Connect (OSTI)

    Froula, Jeff; Zhang, Tao; Salmeen, Annette; Hess, Matthias; Kerfeld, Cheryl A.; Wang, Zhong; Du, Changbin

    2011-06-02

    Fully understanding the genetic potential of a microbial community requires functional annotation of all the genes it encodes. The recently developed deep metagenome sequencing approach has enabled rapid identification of millions of genes from a complex microbial community without cultivation. Current homology-based gene annotation fails to detect distantly-related or structural homologs. Furthermore, homology searches with millions of genes are very computational intensive. To overcome these limitations, we developed rhModeller, a homology-independent software pipeline to efficiently annotate genes from metagenomic sequencing projects. Using cellulases and carbonic anhydrases as two independent test cases, we demonstrated that rhModeller is much faster than HMMER but with comparable accuracy, at 94.5percent and 99.9percent accuracy, respectively. More importantly, rhModeller has the ability to detect novel proteins that do not share significant homology to any known protein families. As {approx}50percent of the 2 million genes derived from the cow rumen metagenome failed to be annotated based on sequence homology, we tested whether rhModeller could be used to annotate these genes. Preliminary results suggest that rhModeller is robust in the presence of missense and frameshift mutations, two common errors in metagenomic genes. Applying the pipeline to the cow rumen genes identified 4,990 novel cellulases candidates and 8,196 novel carbonic anhydrase candidates.In summary, we expect rhModeller to dramatically increase the speed and quality of metagnomic gene annotation.

  9. In-Situ Visualization Experiments with ParaView Cinema in RAGE

    SciTech Connect (OSTI)

    Kares, Robert John

    2015-10-15

    A previous paper described some numerical experiments performed using the ParaView/Catalyst in-situ visualization infrastructure deployed in the Los Alamos RAGE radiation-hydrodynamics code to produce images from a running large scale 3D ICF simulation. One challenge of the in-situ approach apparent in these experiments was the difficulty of choosing parameters likes isosurface values for the visualizations to be produced from the running simulation without the benefit of prior knowledge of the simulation results and the resultant cost of recomputing in-situ generated images when parameters are chosen suboptimally. A proposed method of addressing this difficulty is to simply render multiple images at runtime with a range of possible parameter values to produce a large database of images and to provide the user with a tool for managing the resulting database of imagery. Recently, ParaView/Catalyst has been extended to include such a capability via the so-called Cinema framework. Here I describe some initial experiments with the first delivery of Cinema and make some recommendations for future extensions of Cinema’s capabilities.

  10. Mass and Elite Views on Nuclear Security: US National Security Surveys 1993-1999

    SciTech Connect (OSTI)

    HERRON,KERRY G.; JENKINS-SMITH,HANK C.; HUGHES,SCOTT D.

    2000-06-01

    This is the fourth report in an ongoing series of studies examining how US perspectives about nuclear security are evolving in the post-Cold War era. In Volume 1 the authors present findings from a nationwide telephone survey of randomly selected members of the US general public conducted from 13 September to 14 October 1999. Results are compared to findings from previous surveys in this series conducted in 1993, 1995, and 1997, and trends are analyzed. Key areas of investigation reported in Volume 1 include evolving perceptions of nuclear weapons risks and benefits, preferences for related policy and spending issues, and views about three emerging issue areas: deterrent utility of precision guided munitions; response options to attacks in which mass casualty weapons are used; and expectations about national missile defenses. In this volume they relate respondent beliefs about nuclear security to perceptions of nuclear risks and benefits and to policy preferences. They develop causal models to partially explain key preferences, and they employ cluster analysis to group respondents into four policy relevant clusters characterized by similar views and preferences about nuclear security within each cluster. Systematic links are found among respondent demographic characteristics, perceptions of nuclear risks and benefits, policy beliefs, and security policy and spending preferences. In Volume 2 they provide analysis of in-depth interviews with fifty members of the US security policy community.

  11. Under-sodium viewing technology for improvement of fast-reactor safeguards

    SciTech Connect (OSTI)

    Beddingfield, David H; Gerhart, Jeremy J; Kawakubo, Yoko

    2009-01-01

    The current safeguards approach for fast reactors relies exclusively on maintenance of continuity of knowledge to track the movement of fuel assemblies through these facilities. The remote handling of fuel assemblies, the visual opacity of the liquid metal coolant. and the chemical reactivity of sodium all combine and result in significant limitations on the available options to verify fuel assembly identification numbers or the integrity of these assemblies. These limitations also serve to frustrate attempts to restore the continuity-of-knowledge in instances where the information is under a variety of scenarios. The technology of ultrasonic under-sodium viewing offers new options to the safeguards community for recovering continuity-of-knowledge and applying more traditional item accountancy to fast reactor facilities. We have performed a literature review to investigate the development of under-sodium viewing technologies. In this paper we will summarize our findings and report the state of development of this technology and we will present possible applications to the fast reactor system to improve the existing safeguards approach at these reactors and in future fast reactors.

  12. Wide-angle ITER-prototype tangential infrared and visible viewing system for DIII-D

    SciTech Connect (OSTI)

    Lasnier, C. J. Allen, S. L.; Ellis, R. E.; Fenstermacher, M. E.; McLean, A. G.; Meyer, W. H.; Morris, K.; Seppala, L. G.; Crabtree, K.; Van Zeeland, M. A.

    2014-11-15

    An imaging system with a wide-angle tangential view of the full poloidal cross-section of the tokamak in simultaneous infrared and visible light has been installed on DIII-D. The optical train includes three polished stainless steel mirrors in vacuum, which view the tokamak through an aperture in the first mirror, similar to the design concept proposed for ITER. A dichroic beam splitter outside the vacuum separates visible and infrared (IR) light. Spatial calibration is accomplished by warping a CAD-rendered image to align with landmarks in a data image. The IR camera provides scrape-off layer heat flux profile deposition features in diverted and inner-wall-limited plasmas, such as heat flux reduction in pumped radiative divertor shots. Demonstration of the system to date includes observation of fast-ion losses to the outer wall during neutral beam injection, and shows reduced peak wall heat loading with disruption mitigation by injection of a massive gas puff.

  13. Public views on multiple dimensions of security : nuclear waepons, terrorism, energy, and the environment : 2007.

    SciTech Connect (OSTI)

    Herron, Kerry Gale; Jenkins-Smith, Hank C.

    2008-01-01

    We analyze and compare findings from identical national surveys of the US general public on nuclear security and terrorism administered by telephone and Internet in mid-2007. Key areas of investigation include assessments of threats to US security; valuations of US nuclear weapons and nuclear deterrence; perspectives on nuclear proliferation, including the specific cases of North Korea and Iran; and support for investments in nuclear weapons capabilities. Our analysis of public views on terrorism include assessments of the current threat, progress in the struggle against terrorism, preferences for responding to terrorist attacks at different levels of assumed casualties, and support for domestic policies intended to reduce the threat of terrorism. Also we report findings from an Internet survey conducted in mid 2007 that investigates public views of US energy security, to include: energy supplies and reliability; energy vulnerabilities and threats, and relationships among security, costs, energy dependence, alternative sources, and research and investment priorities. We analyze public assessments of nuclear energy risks and benefits, nuclear materials management issues, and preferences for the future of nuclear energy in the US. Additionally, we investigate environmental issues as they relate to energy security, to include expected implications of global climate change, and relationships among environmental issues and potential policy options.

  14. An opposite view data replacement approach for reducing artifacts due to metallic dental objects

    SciTech Connect (OSTI)

    Yazdi, Mehran; Lari, Meghdad Asadi; Bernier, Gaston; Beaulieu, Luc

    2011-04-15

    Purpose: To present a conceptually new method for metal artifact reduction (MAR) that can be used on patients with multiple objects within the scan plane that are also of small sized along the longitudinal (scanning) direction, such as dental fillings. Methods: The proposed algorithm, named opposite view replacement, achieves MAR by first detecting the projection data affected by metal objects and then replacing the affected projections by the corresponding opposite view projections, which are not affected by metal objects. The authors also applied a fading process to avoid producing any discontinuities in the boundary of the affected projection areas in the sinogram. A skull phantom with and without a variety of dental metal inserts was made to extract the performance metric of the algorithm. A head and neck case, typical of IMRT planning, was also tested. Results: The reconstructed CT images based on this new replacement scheme show a significant improvement in image quality for patients with metallic dental objects compared to the MAR algorithms based on the interpolation scheme. For the phantom, the authors showed that the artifact reduction algorithm can efficiently recover the CT numbers in the area next to the metallic objects. Conclusions: The authors presented a new and efficient method for artifact reduction due to multiple small metallic objects. The obtained results from phantoms and clinical cases fully validate the proposed approach.

  15. Surface enhanced Raman gene probe and methods thereof

    DOE Patents [OSTI]

    Vo-Dinh, T.

    1998-07-21

    The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.

  16. Surface enhanced Raman gene probe and methods thereof

    DOE Patents [OSTI]

    Vo-Dinh, Tuan

    1998-01-01

    The subject invention disclosed herein is a new gene probe biosensor and methods thereof based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays.

  17. Surface enhanced Raman gene probe and methods thereof

    DOE Patents [OSTI]

    Vo-Dinh, T.

    1998-02-24

    The subject invention disclosed is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.

  18. Surface enhanced Raman gene probe and methods thereof

    DOE Patents [OSTI]

    Vo-Dinh, T.

    1998-09-29

    The subject invention disclosed herein is a new gene probe biosensor and methods based on surface enhanced Raman scattering (SERS) label detection. The SER gene probe biosensor comprises a support means, a SER gene probe having at least one oligonucleotide strand labeled with at least one SERS label, and a SERS active substrate disposed on the support means and having at least one of the SER gene probes adsorbed thereon. Biotargets such as bacterial and viral DNA, RNA and PNA are detected using a SER gene probe via hybridization to oligonucleotide strands complementary to the SER gene probe. The support means supporting the SERS active substrate includes a fiberoptic probe, an array of fiberoptic probes for performance of multiple assays and a waveguide microsensor array with charge-coupled devices or photodiode arrays. 18 figs.

  19. Genome-wide experimental determination of barriers to horizontal gene

    Office of Scientific and Technical Information (OSTI)

    transfer (Journal Article) | SciTech Connect Genome-wide experimental determination of barriers to horizontal gene transfer Citation Details In-Document Search Title: Genome-wide experimental determination of barriers to horizontal gene transfer Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to another, has been investigated in microbial species mainly through computational sequence analyses. To address the lack of experimental data, we

  20. Global prevalence and distribution of genes and microorganisms involved in

    Office of Scientific and Technical Information (OSTI)

    mercury methylation (Journal Article) | DOE PAGES Global prevalence and distribution of genes and microorganisms involved in mercury methylation « Prev Next » Title: Global prevalence and distribution of genes and microorganisms involved in mercury methylation Mercury methylation produces the neurotoxic, highly bioaccumulative methylmercury (MeHg). Recent identification of the methylation genes (hgcAB) provides the foundation for broadly evaluating microbial Hg-methylation potential in

  1. IDENTIFYING GENES CONTROLLING FERULATE CROSS-LINKING FORMATION IN GRASS

    Office of Scientific and Technical Information (OSTI)

    CELL WALLS (Technical Report) | SciTech Connect SciTech Connect Search Results Technical Report: IDENTIFYING GENES CONTROLLING FERULATE CROSS-LINKING FORMATION IN GRASS CELL WALLS Citation Details In-Document Search Title: IDENTIFYING GENES CONTROLLING FERULATE CROSS-LINKING FORMATION IN GRASS CELL WALLS DESCRIPTION/ABSTRACT This proposal focuses on cell wall feruloylation and our long term goal is to identify and isolate novel genes controlling feruloylation and to characterize the

  2. Listening to the noise: random fluctuations reveal gene network parameters

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Journal Article: Listening to the noise: random fluctuations reveal gene network parameters Citation Details In-Document Search Title: Listening to the noise: random fluctuations reveal gene network parameters The cellular environment is abuzz with noise. The origin of this noise is attributed to the inherent random motion of reacting molecules that take part in gene expression and post expression interactions. In this noisy environment, clonal populations

  3. Engineering disease resistance with pectate lyase-like genes

    DOE Patents [OSTI]

    Vogel, John; Somerville, Shauna

    2005-03-08

    A mutant gene coding for pectate lyase and homologs thereof is provided, which when incorporated in transgenic plants effect an increased level disease resistance in such plants. Also is provided the polypeptide sequence for the pectate lyase of the present invention. Methods of obtaining the mutant gene, producing transgenic plants which include the nucleotide sequence for the mutant gene and producing improved disease resistance in a crop of such transgenic plants are also provided.

  4. Antibody library project could unlock mysteries of human gene function

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mysteries of human gene function Antibody library project could unlock mysteries of human gene function By looking at antibodies, researchers can identify where, in a cell, genes are active and under what conditions they increase or decrease their expression. October 31, 2011 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy

  5. MAVTgsa: An R Package for Gene Set (Enrichment) Analysis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chien, Chih-Yi; Chang, Ching-Wei; Tsai, Chen-An; Chen, James J.

    2014-01-01

    Gene semore » t analysis methods aim to determine whether an a priori defined set of genes shows statistically significant difference in expression on either categorical or continuous outcomes. Although many methods for gene set analysis have been proposed, a systematic analysis tool for identification of different types of gene set significance modules has not been developed previously. This work presents an R package, called MAVTgsa, which includes three different methods for integrated gene set enrichment analysis. (1) The one-sided OLS (ordinary least squares) test detects coordinated changes of genes in gene set in one direction, either up- or downregulation. (2) The two-sided MANOVA (multivariate analysis variance) detects changes both up- and downregulation for studying two or more experimental conditions. (3) A random forests-based procedure is to identify gene sets that can accurately predict samples from different experimental conditions or are associated with the continuous phenotypes. MAVTgsa computes the P values and FDR (false discovery rate) q -value for all gene sets in the study. Furthermore, MAVTgsa provides several visualization outputs to support and interpret the enrichment results. This package is available online.« less

  6. Mutator gene and hereditary non-polyposis colorectal cancer

    DOE Patents [OSTI]

    de la Chapelle, Albert; Vogelstein, Bert; Kinzler, Kenneth W.

    2008-02-05

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error.sup.+ (RER.sup.+) tumor cells.

  7. Blue Gene/Q Network Performance Counters Monitoring Library

    Energy Science and Technology Software Center (OSTI)

    2015-03-12

    BGQNCL is a library to monitor and record network performance counters on the 5D torus interconnection network of IBM's Blue Gene/Q platform.

  8. Listening to the noise: random fluctuations reveal gene network...

    Office of Scientific and Technical Information (OSTI)

    Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES; EXCITATION; FLUCTUATIONS; GENES; GENETICS; ORIGIN; STATISTICS; NOISE Word Cloud More Like This Full Text preview image File ...

  9. Variant translocation of the bcl-2 gene to immunoglobulin. lambda. light chain gene in chronic lymphocytic leukemia

    SciTech Connect (OSTI)

    Adachi, M.; Cossman, J.; Longo, D.; Croce, C.M.; Tsujimoto, Y. (Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA))

    1989-04-01

    The bcl-2 gene has been identified as a gene directly involved in the consistent chromosome translocation t(14;18), which is found in {approx} 90% of human follicular lymphoma cases, and is a prime candidate for the oncogene playing a crucial role in follicular lymphomagenesis. In this paper, the authors describe a case of chronic lymphocytic leukemia showing the juxtaposition of the bcl-2 gene on chromosome 18 to immunoglobulin {lambda} light chain (Ig{lambda}) gene on chromosome 22 in a head-to-head configuration. Sequencing analysis of the joining site of the bcl-2 gene and Ig{lambda} gene has shown that the breakpoint is within the 5{prime} flanking region of the bcl-2 gene and about 2.2 kilobases 5{prime} to the joining segment of Ig{lambda} locus in a germ-line configuration. The extranucleotide, commonly appearing at the joining site of the t(14;18) translocation involving the IgH locus, is absent from the joining site of bcl-2 and Ig{lambda}. The lack of extranucleotide suggests that the juxtaposition of the bcl-2 and Ig{lambda} genes occurred during physiological rearrangement of the Ig{lambda} gene since it has been shown that the rearrangement of the Ig{lambda} locus is not accompanied by extranucleotides.

  10. Extensive horizontal gene transfer, duplication, and loss of chlorophyll synthesis genes in the algae

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hunsperger, Heather M.; Randhawa, Tejinder; Cattolico, Rose Ann

    2015-02-10

    Two non-homologous, isofunctional enzymes catalyze the penultimate step of chlorophyll a synthesis in oxygenic photosynthetic organisms such as cyanobacteria, eukaryotic algae and land plants: the light independent (LIPOR) and light-dependent (POR) protochlorophyllide oxidoreductases. Whereas the distribution of these enzymes in cyanobacteria and land plants is well understood, the presence, loss, duplication, and replacement of these genes have not been surveyed in the polyphyletic and remarkably diverse eukaryotic algal lineages.

  11. Versioned distributed arrays for resilience in scientific applications: Global view resilience

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chien, A.; Balaji, P.; Beckman, P.; Dun, N.; Fang, A.; Fujita, H.; Iskra, K.; Rubenstein, Z.; Zheng, Z.; Schreiber, R.; et al

    2015-06-01

    Exascale studies project reliability challenges for future high-performance computing (HPC) systems. We propose the Global View Resilience (GVR) system, a library that enables applications to add resilience in a portable, application-controlled fashion using versioned distributed arrays. We describe GVR’s interfaces to distributed arrays, versioning, and cross-layer error recovery. Using several large applications (OpenMC, the preconditioned conjugate gradient solver PCG, ddcMD, and Chombo), we evaluate the programmer effort to add resilience. The required changes are small (<2% LOC), localized, and machine-independent, requiring no software architecture changes. We also measure the overhead of adding GVR versioning and show that generally overheads <2%more » are achieved. We conclude that GVR’s interfaces and implementation are flexible and portable and create a gentle-slope path to tolerate growing error rates in future systems.« less

  12. A Global View Programming Abstraction for Transitioning MPI Codes to PGAS Languages

    SciTech Connect (OSTI)

    Mintz, Tiffany M [ORNL] [ORNL; Hernandez, Oscar R [ORNL] [ORNL; Bernholdt, David E [ORNL] [ORNL

    2014-01-01

    The multicore generation of scientific high performance computing has provided a platform for the realization of Exascale computing, and has also underscored the need for new paradigms in coding parallel applications. The current standard for writing parallel applications requires programmers to use languages designed for sequential execution. These languages have abstractions that only allow programmers to operate on the process centric local view of data. To provide suitable languages for parallel execution, many research efforts have designed languages based on the Partitioned Global Address Space (PGAS) programming model. Chapel is one of the more recent languages to be developed using this model. Chapel supports multithreaded execution with high-level abstractions for parallelism. With Chapel in mind, we have developed a set of directives that serve as intermediate expressions for transitioning scientific applications from languages designed for sequential execution to PGAS languages like Chapel that are being developed with parallelism in mind.

  13. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    SciTech Connect (OSTI)

    Hoen, Ben; Wiser, Ryan; Cappers, Peter; Thayer, Mark; Sethi, Gautam

    2010-04-01

    With wind energy expanding rapidly in the U.S. and abroad, and with an increasing number of communities considering nearby wind power developments, there is a need to empirically investigate community concerns about wind project development. One such concern is that property values may be adversely affected by wind energy facilities, and relatively little existing research exists on the subject. The present research is based on almost 7,500 sales of single-family homes situated within ten miles of 24 existing wind facilities in nine different U.S. states. The conclusions of the study are drawn from four different hedonic pricing models. The model results are consistent in that neither the view of the wind facilities nor the distance of the home to those facilities is found to have a statistically significant effect on home sales prices.

  14. Visible wide angle view imaging system of KTM tokamak based on multielement image fiber bundle

    SciTech Connect (OSTI)

    Chektybayev, B. Shapovalov, G.; Kolodeshnikov, A.

    2015-05-15

    In the paper, new visible wide angle view imaging system of KTM tokamak is described. The system has been designed to observe processes inside of plasma and the processes occurring due to plasma-wall interactions through the long equatorial port. Imaging system is designed based on special image fiber bundle and entrance wide angle lens, which provide image of large section of the vacuum chamber, both poloidal half-section and divertor through the sufficiently long equatorial port. The system also consists of two video cameras: slow and fast with image intensifier. Commercial equipment had been used in design of the system that allowed reducing the cost and time for research and development. The paper also discusses advantages and disadvantages of the system in comparison with conventional endoscopes based on a lens system and considers its promising utilization in future tokamaks and future steady state fusion reactors.

  15. Versioned distributed arrays for resilience in scientific applications: Global view resilience

    SciTech Connect (OSTI)

    Chien, A.; Balaji, P.; Beckman, P.; Dun, N.; Fang, A.; Fujita, H.; Iskra, K.; Rubenstein, Z.; Zheng, Z.; Schreiber, R.; Hammond, J.; Dinan, J.; Laguna, I.; Richards, D.; Dubey, A.; van Straalen, B.; Hoemmen, M.; Heroux, M.; Teranishi, K.; Siegel, A.

    2015-06-01

    Exascale studies project reliability challenges for future high-performance computing (HPC) systems. We propose the Global View Resilience (GVR) system, a library that enables applications to add resilience in a portable, application-controlled fashion using versioned distributed arrays. We describe GVRs interfaces to distributed arrays, versioning, and cross-layer error recovery. Using several large applications (OpenMC, the preconditioned conjugate gradient solver PCG, ddcMD, and Chombo), we evaluate the programmer effort to add resilience. The required changes are small (<2% LOC), localized, and machine-independent, requiring no software architecture changes. We also measure the overhead of adding GVR versioning and show that generally overheads <2% are achieved. We conclude that GVRs interfaces and implementation are flexible and portable and create a gentle-slope path to tolerate growing error rates in future systems.

  16. Siting landfills and incinerators in areas of historic unpopularity: Surveying the views of the next generation

    SciTech Connect (OSTI)

    De Feo, Giovanni; Williams, Ian D.

    2013-12-15

    Highlights: Opinions and knowledge of young people in Italy about waste were studied. Historic opposition to construction of waste facilities is difficult to overcome. Awareness of waste management develops with knowledge of environmental issues. Many stakeholders views are needed when siting a new waste management facility. Respondents opinions were influenced by their level of environmental knowledge. - Abstract: The Campania Region in Southern Italy has suffered many problems with municipal solid waste management since the mid-1990s, leading to significant public disturbances and subsequent media coverage. This paper reports on the current views and knowledge of young people (university students) in this region about waste management operations and facilities, specifically the siting of landfills and incinerators. By means of a structured questionnaire, opinion and knowledge were systematically examined by degree type and course year. The study took place in 2011 at the University of Salerno campus. A sample of 900 students, comprising 100 students for each of the nine considered faculties, and 20 students for every academic course year, was randomly selected. Only about a quarter of respondents were not opposed to the siting of a landfill or an incinerator in their city. This clearly highlights that historic opposition to the construction of waste facilities is difficult to overcome and that distrust for previous poor management or indiscretions is long-lived and transcends generations. Students from technical faculties expressed the most reasonable opinion; opinion and knowledge were statistically related (Chi-square test, p < 0.05) to the attended faculty, and the knowledge grew linearly with progression through the university. This suggests that awareness of waste management practices develops with experience and understanding of environmental issues. There is general acceptance that many stakeholders technicians, politicians and citizens all have to be part of the decision process when siting a new waste management facility. The opinions of the young respondents were significantly influenced by their level of environmental knowledge.

  17. On the operation of X-ray polarimeters with a large field of view

    SciTech Connect (OSTI)

    Muleri, Fabio, E-mail: fabio.muleri@iaps.inaf.it [INAF-IAPS, Via del Fosso del Cavaliere 100, I-00133 Roma (Italy)

    2014-02-10

    The measurement of linear polarization is one of the hot topics of high-energy astrophysics. Gas detectors based on the photoelectric effect have paved the way for the design of sensitive instruments, and mission proposals based on them have been presented in the last few years in the energy range from about 2 keV to a few tens of keV. In addition, a number of polarimeters based on Compton scattering are approved or being discussed for launch on board balloons or space satellites at higher energies. These instruments are typically dedicated to pointed observations with narrow field of view telescopes or collimators, but there are also projects aimed at the polarimetry of bright transient sources such as soft gamma repeaters or the prompt emission of gamma-ray bursts. Given the erratic appearance of such events in the sky, these polarimeters have large fields of view to catch a reasonable number of them, and as a result, photons may impinge on the detector off-axis. This dramatically changes the response of the instrument to polarization, regardless of whether photoabsorption or Compton scattering is involved. Instead of the simple cosine-squared dependence expected for polarized photons that are incident on-axis, the response is never purely cosinusoidal, and a systematic modulation also appears for unpolarized radiation. We investigate the origin of these differences and present an analytical treatment that proves that such systematic effects are actually a natural consequence of how current instruments operate. Our analysis provides the expected response of photoelectric or Compton polarimeters to photons impinging with any inclination and state of polarization.

  18. A MULTI-SPACECRAFT VIEW OF A GIANT FILAMENT ERUPTION DURING 2009 SEPTEMBER 26/27

    SciTech Connect (OSTI)

    Gosain, Sanjay; Schmieder, Brigitte; Artzner, Guy; Bogachev, Sergei; Toeroek, Tibor

    2012-12-10

    We analyze multi-spacecraft observations of a giant filament eruption that occurred during 2009 September 26 and 27. The filament eruption was associated with a relatively slow coronal mass ejection. The filament consisted of a large and a small part, and both parts erupted nearly simultaneously. Here we focus on the eruption associated with the larger part of the filament. The STEREO satellites were separated by about 117 Degree-Sign during this event, so we additionally used SoHO/EIT and CORONAS/TESIS observations as a third eye (Earth view) to aid our measurements. We measure the plane-of-sky trajectory of the filament as seen from STEREO-A and TESIS viewpoints. Using a simple trigonometric relation, we then use these measurements to estimate the true direction of propagation of the filament which allows us to derive the true R/R{sub Sun }-time profile of the filament apex. Furthermore, we develop a new tomographic method that can potentially provide a more robust three-dimensional (3D) reconstruction by exploiting multiple simultaneous views. We apply this method also to investigate the 3D evolution of the top part of filament. We expect this method to be useful when SDO and STEREO observations are combined. We then analyze the kinematics of the eruptive filament during its rapid acceleration phase by fitting different functional forms to the height-time data derived from the two methods. We find that for both methods an exponential function fits the rise profile of the filament slightly better than parabolic or cubic functions. Finally, we confront these results with the predictions of theoretical eruption models.

  19. GenePRIMP: A software quality control tool

    SciTech Connect (OSTI)

    Amrita Pati

    2010-05-05

    Amrita Pati of the DOE Joint Genome Institute's Genome Biology group describes the software tool GenePRIMP and how it fits into the quality control pipeline for microbial genomics. Further details regarding GenePRIMP appear in a paper published online May 2, 2010 in Nature Methods.

  20. GenePRIMP: A software quality control tool

    ScienceCinema (OSTI)

    Amrita Pati

    2010-09-01

    Amrita Pati of the DOE Joint Genome Institute's Genome Biology group describes the software tool GenePRIMP and how it fits into the quality control pipeline for microbial genomics. Further details regarding GenePRIMP appear in a paper published online May 2, 2010 in Nature Methods.

  1. Prediction of epigenetically regulated genes in breast cancer cell lines

    SciTech Connect (OSTI)

    Loss, Leandro A; Sadanandam, Anguraj; Durinck, Steffen; Nautiyal, Shivani; Flaucher, Diane; Carlton, Victoria EH; Moorhead, Martin; Lu, Yontao; Gray, Joe W; Faham, Malek; Spellman, Paul; Parvin, Bahram

    2010-05-04

    Methylation of CpG islands within the DNA promoter regions is one mechanism that leads to aberrant gene expression in cancer. In particular, the abnormal methylation of CpG islands may silence associated genes. Therefore, using high-throughput microarrays to measure CpG island methylation will lead to better understanding of tumor pathobiology and progression, while revealing potentially new biomarkers. We have examined a recently developed high-throughput technology for measuring genome-wide methylation patterns called mTACL. Here, we propose a computational pipeline for integrating gene expression and CpG island methylation profles to identify epigenetically regulated genes for a panel of 45 breast cancer cell lines, which is widely used in the Integrative Cancer Biology Program (ICBP). The pipeline (i) reduces the dimensionality of the methylation data, (ii) associates the reduced methylation data with gene expression data, and (iii) ranks methylation-expression associations according to their epigenetic regulation. Dimensionality reduction is performed in two steps: (i) methylation sites are grouped across the genome to identify regions of interest, and (ii) methylation profles are clustered within each region. Associations between the clustered methylation and the gene expression data sets generate candidate matches within a fxed neighborhood around each gene. Finally, the methylation-expression associations are ranked through a logistic regression, and their significance is quantified through permutation analysis. Our two-step dimensionality reduction compressed 90% of the original data, reducing 137,688 methylation sites to 14,505 clusters. Methylation-expression associations produced 18,312 correspondences, which were used to further analyze epigenetic regulation. Logistic regression was used to identify 58 genes from these correspondences that showed a statistically signifcant negative correlation between methylation profles and gene expression in the panel of breast cancer cell lines. Subnetwork enrichment of these genes has identifed 35 common regulators with 6 or more predicted markers. In addition to identifying epigenetically regulated genes, we show evidence of differentially expressed methylation patterns between the basal and luminal subtypes. Our results indicate that the proposed computational protocol is a viable platform for identifying epigenetically regulated genes. Our protocol has generated a list of predictors including COL1A2, TOP2A, TFF1, and VAV3, genes whose key roles in epigenetic regulation is documented in the literature. Subnetwork enrichment of these predicted markers further suggests that epigenetic regulation of individual genes occurs in a coordinated fashion and through common regulators.

  2. Gene and translation initiation site prediction in metagenomic sequences

    SciTech Connect (OSTI)

    Hyatt, Philip Douglas; LoCascio, Philip F; Hauser, Loren John; Uberbacher, Edward C

    2012-01-01

    Gene prediction in metagenomic sequences remains a difficult problem. Current sequencing technologies do not achieve sufficient coverage to assemble the individual genomes in a typical sample; consequently, sequencing runs produce a large number of short sequences whose exact origin is unknown. Since these sequences are usually smaller than the average length of a gene, algorithms must make predictions based on very little data. We present MetaProdigal, a metagenomic version of the gene prediction program Prodigal, that can identify genes in short, anonymous coding sequences with a high degree of accuracy. The novel value of the method consists of enhanced translation initiation site identification, ability to identify sequences that use alternate genetic codes and confidence values for each gene call. We compare the results of MetaProdigal with other methods and conclude with a discussion of future improvements.

  3. Genomic analyses of bacterial porin-cytochrome gene clusters

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shi, Liang; Fredrickson, James K.; Zachara, John M.

    2014-11-26

    In this study, the porin-cytochrome (Pcc) protein complex is responsible for trans-outer membrane electron transfer during extracellular reduction of Fe(III) by the dissimilatory metal-reducing bacterium Geobacter sulfurreducens PCA. The identified and characterized Pcc complex of G. sulfurreducens PCA consists of a porin-like outer-membrane protein, a periplasmic 8-heme c type cytochrome (c-Cyt) and an outer-membrane 12-heme c-Cyt, and the genes encoding the Pcc proteins are clustered in the same regions of genome (i.e., the pcc gene clusters) of G. sulfurreducens PCA. A survey of additionally microbial genomes has identified the pcc gene clusters in all sequenced Geobacter spp. and other bacteriamore » from six different phyla, including Anaeromyxobacter dehalogenans 2CP-1, A. dehalogenans 2CP-C, Anaeromyxobacter sp. K, Candidatus Kuenenia stuttgartiensis, Denitrovibrio acetiphilus DSM 12809, Desulfurispirillum indicum S5, Desulfurivibrio alkaliphilus AHT2, Desulfurobacterium thermolithotrophum DSM 11699, Desulfuromonas acetoxidans DSM 684, Ignavibacterium album JCM 16511, and Thermovibrio ammonificans HB-1. The numbers of genes in the pcc gene clusters vary, ranging from two to nine. Similar to the metal-reducing (Mtr) gene clusters of other Fe(III)-reducing bacteria, such as Shewanella spp., additional genes that encode putative c-Cyts with predicted cellular localizations at the cytoplasmic membrane, periplasm and outer membrane often associate with the pcc gene clusters. This suggests that the Pcc-associated c-Cyts may be part of the pathways for extracellular electron transfer reactions. The presence of pcc gene clusters in the microorganisms that do not reduce solid-phase Fe(III) and Mn(IV) oxides, such as D. alkaliphilus AHT2 and I. album JCM 16511, also suggests that some of the pcc gene clusters may be involved in extracellular electron transfer reactions with the substrates other than Fe(III) and Mn(IV) oxides.« less

  4. Prairie View A&M Tops 2016 U.S. Department of Energy Race to Zero Student

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Design Competition | Department of Energy Prairie View A&M Tops 2016 U.S. Department of Energy Race to Zero Student Design Competition Prairie View A&M Tops 2016 U.S. Department of Energy Race to Zero Student Design Competition April 22, 2016 - 3:00pm Addthis As part of the Obama Administration's commitment to helping families across the United States save money by saving energy, the U.S. Department of Energy announced winners of its third annual Race to Zero Student Design

  5. Characterization of a constrained paired-view technique in iterative reconstruction for breast tomosynthesis

    SciTech Connect (OSTI)

    Wu, Gang; Yaffe, Martin J.; Mainprize, James G.

    2013-10-15

    Purpose: The order in which the projection views are employed in the reconstruction of tomosynthesis by iterative algorithms, such as simultaneous algebraic reconstruction technique and maximum likelihood, has a strong effect on the rate of convergence, accuracy, and the edge-blurring artifacts in the reconstructed image. The purpose of this investigation was to characterize and evaluate the effects of ordering schemes on image quality for breast tomosynthesis reconstruction and to explore a new constrained paired-view technique that could provide reduction of reconstruction artifacts. In this work, the authors compared several different ordering schemes and characterized the image quality and the formation of out-of-plane artifacts. Furthermore, a new normalization method is presented. It produces more accurate reconstructions with reduced artifacts comparing to the standard method of sequential ordering.Methods: In addition to visual assessment of image quality, several indices such as the signal-difference-to-noise ratio, the artifact-spread function, and the lesion detectability (d{sup ?}) were computed to quantitatively evaluate the effect of ordering scheme. The sets of breast tomosynthesis projection images were simulated for reconstruction; one set had uniform background (white noise only) and the other two contained both anatomic background and quantum noise. Clinical breast images were also studied for comparison.Results: The authors have quantified the image quality in reconstructed slices for a range of tumor sizes. The authors proposed method provides better performance for all of the metrics tested (contrast, d{sup ?}, and the level of artifacts) both for the uniform phantom case and in the presence of anatomical structure.Conclusions: The paired projection normalization provides better performance in the image quality of the reconstructed slices, and results in a lower level of artifacts in the Z direction. This implies that even a relatively simple method like the side-to-side sequence, which pairs two symmetrical projections with equal angular distance from the central projection, would achieve better reconstructed image quality than the conventional step-by-step method, which uses sequential projections one after another.

  6. LATERAL GENE TRANSFER AND THE HISTORY OF BACTERIAL GENOMES

    SciTech Connect (OSTI)

    Howard Ochman

    2006-02-22

    The aims of this research were to elucidate the role and extent of lateral transfer in the differentiation of bacterial strains and species, and to assess the impact of gene transfer on the evolution of bacterial genomes. The ultimate goal of the project is to examine the dynamics of a core set of protein-coding genes (i.e., those that are distributed universally among Bacteria) by developing conserved primers that would allow their amplification and sequencing in any bacterial taxa. In addition, we adopted a bioinformatic approach to elucidate the extent of lateral gene transfer in sequenced genome.

  7. Sustainable development: Background an represent policy views for governmental agencies, industry, and other specialty groups

    SciTech Connect (OSTI)

    Dickerman, J.A.; Silverman, G.S.

    1995-12-01

    Sustainable development is a phrase that has come into common usage without benefit of clear definition or meaning. Usage very much reflects individual and group perspectives: foresters might consider sustainability in terms of maintaining ecological integrity as part of managing forests for wood harvesting, industry might emphasize pollution control, while government agencies may be looking for new ways to exploit resources on a more continuous basis. Perhaps the greatest commonality among groups considering these issues is that {open_quotes}sustainability{close_quotes} has not been attained but that it needs to occur. The National Association of Environmental Professionals (NAEP) agrees that it is critical to the health of the planet that sustainable development be actively pursued and implemented in international, national, regional, and local policies and practices. To contribute to this effort a {open_quotes}white paper{close_quotes} is being prepared. Its purpose is twofold: (1) to review the existing information from the NAEP Sustainable Development Working Group and the literature and through examination of these policies, to clarify the thinking, what is being done, and what is still needed; and (2) to develop a position and action plan. This action plan should direct NAEP`s actions in making a significant contribution to the national dialog. This paper presents the background and results of the review phase of this white paper development. Representative views on sustainable development policy and practice are presented from three perspectives: governmental agencies, industry, and other specialty groups.

  8. A Pan-STARRS1 VIEW OF THE BIFURCATED SAGITTARIUS STREAM

    SciTech Connect (OSTI)

    Slater, C. T.; Bell, E. F.; Schlafly, E. F.; Juric, M.; Finkbeiner, D. P.; Martin, N. F.; Rix, H.-W.; Goldman, B.; Morganson, E. P.; Bernard, E. J.; Burgett, W. S.; Chambers, K. C.; Kaiser, N.; Magnier, E. A.; Tonry, J. L.; Price, P. A.

    2013-01-01

    We use data from the Pan-STARRS1 survey to present a panoramic view of the Sagittarius tidal stream in the southern Galactic hemisphere. As a result of the extensive sky coverage of Pan-STARRS1, the southern stream is visible along more than 60 Degree-Sign of its orbit, nearly double the length seen by the Sloan Digital Sky Survey. The recently discovered southern bifurcation of the stream is also apparent, with the fainter branch of the stream visible over at least 30 Degree-Sign . Using a combination of fitting both the main-sequence turnoff and the red clump, we measure the distance to both arms of the stream in the south. We find that the distances to the bright arm of the stream agree very well with the N-body models of Law and Majewski. We also find that the faint arm lies {approx}5 kpc closer to the Sun than the bright arm, similar to the behavior seen in the northern hemisphere.

  9. LabVIEW-based control software for para-hydrogen induced polarization instrumentation

    SciTech Connect (OSTI)

    Agraz, Jose Grunfeld, Alexander; Li, Debiao; BIRI, Cedars Sinai Medical Center, West Hollywood, California 90048 ; Cunningham, Karl; Willey, Cindy; Pozos, Robert; Wagner, Shawn

    2014-04-15

    The elucidation of cell metabolic mechanisms is the modern underpinning of the diagnosis, treatment, and in some cases the prevention of disease. Para-Hydrogen induced polarization (PHIP) enhances magnetic resonance imaging (MRI) signals over 10 000 fold, allowing for the MRI of cell metabolic mechanisms. This signal enhancement is the result of hyperpolarizing endogenous substances used as contrast agents during imaging. PHIP instrumentation hyperpolarizes Carbon-13 ({sup 13}C) based substances using a process requiring control of a number of factors: chemical reaction timing, gas flow, monitoring of a static magnetic field (B{sub o}), radio frequency (RF) irradiation timing, reaction temperature, and gas pressures. Current PHIP instruments manually control the hyperpolarization process resulting in the lack of the precise control of factors listed above, resulting in non-reproducible results. We discuss the design and implementation of a LabVIEW based computer program that automatically and precisely controls the delivery and manipulation of gases and samples, monitoring gas pressures, environmental temperature, and RF sample irradiation. We show that the automated control over the hyperpolarization process results in the hyperpolarization of hydroxyethylpropionate. The implementation of this software provides the fast prototyping of PHIP instrumentation for the evaluation of a myriad of {sup 13}C based endogenous contrast agents used in molecular imaging.

  10. Quantifying and Mapping the Digital Divide from an Internet Point of View

    SciTech Connect (OSTI)

    Cottrell, R.Les; Khan, Shahryar; Williams, Jerrod; Mehdi, Akbar; Kalim, Umar; Ali, Arshad; /NUST, Rawalpindi

    2008-09-18

    Quantitative knowledge of the magnitude, extent and trends of the Digital Divide are critical to understand and identify the regions most in need of help, to make rational decisions on how to address the problems and to make cases for executives, funding agencies and politicians. We report on a project (PingER) to measure the Digital Divide from the point of view of Internet performance. The PingER project has been measuring Internet performance since 1995 and with the increased emphasis on measuring and tracking the Digital Divide, it now covers over 700 hosts in over 150 countries that between them contain over 99% of the world's Internet connected population. In this paper we will describe the how PingER works, it deployment, the data analysis, and presentation. We also introduce a new PingER visualization tool (ViPER) that provides a more appealing interactive visualization of the PingER data and also works on mobile PDAs. We will also show results from PingER that illustrate the magnitude, extent and trends for the Digital Divide, and also compare PingER results with some human development and technology indices.

  11. New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Guss, Adam M.; Rother, Michael; Zhang, Jun Kai; Kulkkarni, Gargi; Metcalf, William W.

    2008-01-01

    A highly efficient method for chromosomal integration of cloned DNA into Methanosarcina spp. was developed utilizing the site-specific recombination system from the Streptomyces phage φC31. Host strains expressing the φC31 integrase gene and carrying an appropriate recombination site can be transformed with non-replicating plasmids carrying the complementary recombination site at efficiencies similar to those obtained with self-replicating vectors. We have also constructed a series of hybrid promoters that combine the highly expressed M. barkeri P mcrB promoter with binding sites for the tetracycline-responsive, bacterial TetR protein. These promoters are tightly regulated by the presence or absence of tetracycline inmore » strains that express the tetR gene. The hybrid promoters can be used in genetic experiments to test gene essentiality by placing a gene of interest under their control. Thus, growth of strains with tetR -regulated essential genes becomes tetracycline-dependent. A series of plasmid vectors that utilize the site-specific recombination system for construction of reporter gene fusions and for tetracycline regulated expression of cloned genes are reported. These vectors were used to test the efficiency of translation at a variety of start codons. Fusions using an ATG start site were the most active, whereas those using GTG and TTG were approximately one half or one fourth as active, respectively. The CTG fusion was 95% less active than the ATG fusion.« less

  12. Single, Key Gene Discovery Could Streamline Production of Biofuels

    Broader source: Energy.gov [DOE]

    WASHINGTON, DC -- A team of researchers at the Department of Energy’s BioEnergy Science Center (BESC) have pinpointed the exact, single gene that controls ethanol production capacity in a...

  13. Optimizing for Blue Gene/Q Hal Finkel

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for Blue Gene/Q Hal Finkel hfinkel@anl.gov 2015-05-19 Optimizing for Blue Gene/Q ✔ Relevant information on the BG/Q ✔ How you can optimize your code for the BG/Q ✔ Q&A You want to know how to make me compute quickly... Optimizing for Blue Gene/Q This is a BG/Q node This is not Optimizing for Blue Gene/Q This is a BG/Q node Mira has 49152 of these functioning as compute nodes! What programs do... ✔ Read data from memory ✔ Compute using that data ✔ Write results back to memory ✔

  14. Induction of gene expression using a high concentration sugar mixture

    DOE Patents [OSTI]

    England, George R.; Kelley, Aaron; Mitchinson, Colin

    2015-05-19

    Described herein is a composition useful for inducing expression of genes whose expression is under control of an inducible promoter sequence and methods for the compositions preparation and use.

  15. Induction of gene expression using a high concentration sugar mixture

    DOE Patents [OSTI]

    England, George R.; Kelley, Aaron; Mitchinson, Colin

    2010-05-11

    Described herein is a composition useful for inducing expression of genes whose expression is under control of an inducible promoter sequence and methods for the compositions preparation and use.

  16. Visually Relating Gene Expression and in vivo DNA Binding Data

    SciTech Connect (OSTI)

    Huang, Min-Yu; Mackey, Lester; Ker?; nen, Soile V. E.; Weber, Gunther H.; Jordan, Michael I.; Knowles, David W.; Biggin, Mark D.; Hamann, Bernd

    2011-09-20

    Gene expression and in vivo DNA binding data provide important information for understanding gene regulatory networks: in vivo DNA binding data indicate genomic regions where transcription factors are bound, and expression data show the output resulting from this binding. Thus, there must be functional relationships between these two types of data. While visualization and data analysis tools exist for each data type alone, there is a lack of tools that can easily explore the relationship between them. We propose an approach that uses the average expression driven by multiple of ciscontrol regions to visually relate gene expression and in vivo DNA binding data. We demonstrate the utility of this tool with examples from the network controlling early Drosophila development. The results obtained support the idea that the level of occupancy of a transcription factor on DNA strongly determines the degree to which the factor regulates a target gene, and in some cases also controls whether the regulation is positive or negative.

  17. Uses of antimicrobial genes from microbial genome (Patent) |...

    Office of Scientific and Technical Information (OSTI)

    of such genes can be used as antimicrobial agents or as tools for molecular biology. ... DOE Contract Number: AC02-05CH11231 Resource Type: Patent Research Org: OSTI (Office of ...

  18. Uses of antimicrobial genes from microbial genome (Patent) |...

    Office of Scientific and Technical Information (OSTI)

    The products of such genes can be used as antimicrobial agents or as tools for molecular biology. Authors: Sorek, Rotem ; Rubin, Edward M. Publication Date: 2013-08-20 OSTI ...

  19. Genome Enabled Discovery of Carbon Sequestration Genes in Poplar

    SciTech Connect (OSTI)

    Filichkin, Sergei; Etherington, Elizabeth; Ma, Caiping; Strauss, Steve

    2007-02-22

    The goals of the S.H. Strauss laboratory portion of 'Genome-enabled discovery of carbon sequestration genes in poplar' are (1) to explore the functions of candidate genes using Populus transformation by inserting genes provided by Oakridge National Laboratory (ORNL) and the University of Florida (UF) into poplar; (2) to expand the poplar transformation toolkit by developing transformation methods for important genotypes; and (3) to allow induced expression, and efficient gene suppression, in roots and other tissues. As part of the transformation improvement effort, OSU developed transformation protocols for Populus trichocarpa 'Nisqually-1' clone and an early flowering P. alba clone, 6K10. Complete descriptions of the transformation systems were published (Ma et. al. 2004, Meilan et. al 2004). Twenty-one 'Nisqually-1' and 622 6K10 transgenic plants were generated. To identify root predominant promoters, a set of three promoters were tested for their tissue-specific expression patterns in poplar and in Arabidopsis as a model system. A novel gene, ET304, was identified by analyzing a collection of poplar enhancer trap lines generated at OSU (Filichkin et. al 2006a, 2006b). Other promoters include the pGgMT1 root-predominant promoter from Casuarina glauca and the pAtPIN2 promoter from Arabidopsis root specific PIN2 gene. OSU tested two induction systems, alcohol- and estrogen-inducible, in multiple poplar transgenics. Ethanol proved to be the more efficient when tested in tissue culture and greenhouse conditions. Two estrogen-inducible systems were evaluated in transgenic Populus, neither of which functioned reliably in tissue culture conditions. GATEWAY-compatible plant binary vectors were designed to compare the silencing efficiency of homologous (direct) RNAi vs. heterologous (transitive) RNAi inverted repeats. A set of genes was targeted for post transcriptional silencing in the model Arabidopsis system; these include the floral meristem identity gene (APETALA1 or AP1), auxin response factor gene (ETTIN), the gene encoding transcriptional factor of WD40 family (TRANSPARENTTESTAGLABRA1 or TTG1), and the auxin efflux carrier (PIN-FORMED2 or PIN2) gene. More than 220 transgenic lines of the 1st, 2nd and 3rd generations were analyzed for RNAi suppression phenotypes (Filichkin et. al., manuscript submitted). A total of 108 constructs were supplied by ORNL, UF and OSU and used to generate over 1,881 PCR verified transgenic Populus and over 300 PCR verified transgenic Arabidopsis events. The Populus transgenics alone required Agrobacterium co-cultivations of 124.406 explants.

  20. New Mathematical Method Reveals Where Genes Switch On or Off

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New Mathematical Method Reveals Where Genes Switch On or Off New Mathematical Method Reveals Where Genes Switch On or Off "Compressed sensing" determines atomic-level energy potentials with accuracy approaching experimental measurement February 22, 2012 John Hules, JAHules@lbl.gov, +1 510 486 6008 Figure 1. Helix-turn-helix (HTH) proteins are the most widely distributed family of DNA-binding proteins, occurring in all biological kingdoms. This image shows a lambda repressor HTH