Sample records for gene transfection view

  1. Variability in the Stability and Productivity of Transfected Genes in Chinese Hamster Ovary (CHO) cells

    E-Print Network [OSTI]

    Ng, Say Kong

    In the field of biologics production, productivity and stability of the transfected gene of interest are two very important attributes that dictate if a production process is viable. To further understand and improve these ...

  2. Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection

    SciTech Connect (OSTI)

    Daniela Rodica Radu

    2005-12-19T23:59:59.000Z

    The central theme of this dissertation is represented by the versatility of mesoporous silica nanomaterials in various applications such as catalysis and bio-applications, with main focus on biological applications of Mesoporous Silica Nanospheres (MSN). The metamorphosis that we impose to these materials from catalysis to sensing and to drug and gene delivery is detailed in this dissertation. First, we developed a synthetic method that can fine tune the amount of chemically accessible organic functional groups on the pores surface of MSN by exploiting electrostatic and size matching between the cationic alkylammonium head group of the cetyltrimethylammonium bromide (CTAB) surfactant and various anionic organoalkoxysilane precursors at the micelle-water interface in a base-catalyzed condensation reaction of silicate. Aiming nature imitation, we demonstrated the catalytic abilities of the MSNs, We utilized an ethylenediamine functional group for chelating Cu{sup 2+} as a catalytic functional group anchored inside the mesopores. Thus, a polyalkynylene-based conducting polymer (molecular wire) was synthesized within the Cu-functionalized MSNs silica catalyst. For sensing applications, we have synthesized a poly(lactic acid) coated mesoporous silica nanosphere (PLA-MSN) material that serves as a fluorescence sensor system for detection of amino-containing neurotransmitters in neutral aqueous buffer. We exploited the mesoporosity of MSNs for encapsulating pharmaceutical drugs. We examined bio-friendly capping molecules such as polyamidoamine dendrimers of generations G2 to G4, to prevent the drug leaching. Next, the drug delivery system employed MSNs loaded with Doxorubicin, an anticancer drug. The results demonstrated that these nano-Trojan horses have ability to deliver Doxorubicin to cancer cells and induce their death. Finally, to demonstrate the potential of MSN as an universal cellular transmembrane nanovehicle, we anchored positively charged dendrimers on the surface of MSN and utilize them to complex cationic DNA. The p-EGFP-CI gene-coated MSN nanocomposite was able to transfect cancer cell lines, such as human HeLa and CHO cancer cell lines. The gene carrier ability of MSNs was further proved by transfecting primary cells and cotransfecting of two different genes in cancer cell lines. In sum, MSN are versatile partners in several types of applications.

  3. NEWS AND VIEWS Modeling gene expression control using Omes Law

    E-Print Network [OSTI]

    Nguyen, Dat H.

    NEWS AND VIEWS Modeling gene expression control using Omes Law Harmen J Bussemaker Department class of methods that fit simple mathematical models of transcription regulation to DNA microarray data factors (TFs) to specific sites in the genome is a crucial step in the molecular process controlling gene

  4. Fibre based cellular transfection

    E-Print Network [OSTI]

    2008-01-01T23:59:59.000Z

    320.7090) Ultra fast lasers; (190.4180) Multiphotonlaser transfection technique to date. It leads to a sterile and ultra-ultra-short pulse delivery. Additionally our technique paves the way for potential laser

  5. Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles

    E-Print Network [OSTI]

    O'Brien, John A; Lummis, Sarah C R

    2011-06-10T23:59:59.000Z

    to transfect neurones (some of which are notor- iously difficult to transfect), cells deep in tissues (DNA can be carried considerable distances through other cells such as layers of skin), and bacteria [4-7]. Currently there is much interest in its use... spread of the gold parti- cles, which were subsequently dried with a flow of nitro- gen. The tubing was cut using a tubing cutter (Bio-Rad) into 1 cm lengths to create bullets, which were either used immediately or stored with desiccant at 4°C until...

  6. Characterization of cell lines stably transfected with rubella virus replicons

    SciTech Connect (OSTI)

    Tzeng, Wen-Pin; Xu, Jie [Department of Biology, Georgia State University, P.O. Box 4010, Atlanta GA 30302-4010 (United States)] [Department of Biology, Georgia State University, P.O. Box 4010, Atlanta GA 30302-4010 (United States); Frey, Teryl K., E-mail: tfrey@gsu.edu [Department of Biology, Georgia State University, P.O. Box 4010, Atlanta GA 30302-4010 (United States)

    2012-07-20T23:59:59.000Z

    Rubella virus (RUBV) replicons expressing a drug resistance gene and a gene of interest were used to select cell lines uniformly harboring the replicon. Replicons expressing GFP and a virus capsid protein GFP fusion (C-GFP) were compared. Vero or BHK cells transfected with either replicon survived drug selection and grew into a monolayer. However, survival was {approx}9-fold greater following transfection with the C-GFP-replicon than with the GFP-expressing replicon and while the C-GFP-replicon cells grew similarly to non-transfected cells, the GFP-replicon cells grew slower. Neither was due to the ability of the CP to enhance RNA synthesis but survival during drug selection was correlated with the ability of CP to inhibit apoptosis. Additionally, C-GFP-replicon cells were not cured of the replicon in the absence of drug selection. Interferon-alpha suppressed replicon RNA and protein synthesis, but did not cure the cells, explaining in part the ability of RUBV to establish persistent infections.

  7. pBabe Retrovirus Production 293 Transfection

    E-Print Network [OSTI]

    Ye, Jianping

    pBabe Retrovirus Production 293 Transfection 1. Plate 12 x 10 6 293.T in 20 ml on a 15 cm 2/N with no shaking. 11. To resuspend, hold tube at angle and pipet fluid over pellet 20 times, being careful not to touch pellet with tip. It is expected that the pellet not be resuspended after this is complete

  8. April 2013 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    Delivery and Gene Transfection Daniela Rodica Radu (2005) 84 > Advisory Committee on human radiation experiments. Supplemental Volume 2a, Sources and documentation appendices....

  9. de Lange Lab Calcium Phosphate Transfection of 293T Cells

    E-Print Network [OSTI]

    de Lange, Titia

    resuspend the cells by pipetting up and down 3-5 times. Transfer to a 1.5-ml Eppendorf tube. Vortex for 3de Lange Lab 1 Calcium Phosphate Transfection of 293T Cells Required Solutions and Reagents Lysis 3 times with PBS, resuspend the beads into equal volume of PBS, add sodium azide to 0.02% and store

  10. Nanoprobe implantation into mammalian cells by cationic transfection Yan Zhao,a Bryce Sadtler,a Min Lin,b Gregory H. Hockermanb and Alexander Wei*a

    E-Print Network [OSTI]

    Wei, Alexander

    imaging.4 As part of our efforts to design nanoprobes as sensors for intracellular chemical transport, we and near-infrared light, and can serve as substrates for plasmon-enhanced spectroscopies such as surface. Cationic transfection was evaluated on tsA201 cells using three different systems (TransIT®-293, Gene

  11. Reduced expression of Autographa californica nucleopolyhedrovirus ORF34, an essential gene, enhances heterologous gene expression

    SciTech Connect (OSTI)

    Salem, Tamer Z. [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States) [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States); Department of Microbial Molecular Biology, AGERI, Agricultural Research Center, Giza 12619 (Egypt); Division of Biomedical Sciences, Zewail University, Zewail City of Science and Technology, Giza 12588 (Egypt); Zhang, Fengrui [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States)] [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States); Thiem, Suzanne M., E-mail: smthiem@msu.edu [Department of Entomology, Michigan State University, East Lansing, MI 48824 (United States); Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824 (United States)

    2013-01-20T23:59:59.000Z

    Autographa californica multiple nucleopolyhedrovirus ORF34 is part of a transcriptional unit that includes ORF32, encoding a viral fibroblast growth factor (FGF) and ORF33. We identified ORF34 as a candidate for deletion to improve protein expression in the baculovirus expression system based on enhanced reporter gene expression in an RNAi screen of virus genes. However, ORF34 was shown to be an essential gene. To explore ORF34 function, deletion (KO34) and rescue bacmids were constructed and characterized. Infection did not spread from primary KO34 transfected cells and supernatants from KO34 transfected cells could not infect fresh Sf21 cells whereas the supernatant from the rescue bacmids transfection could recover the infection. In addition, budded viruses were not observed in KO34 transfected cells by electron microscopy, nor were viral proteins detected from the transfection supernatants by western blots. These demonstrate that ORF34 is an essential gene with a possible role in infectious virus production.

  12. Gene silencing in cancer cells using siRNA : genetic and functional studies 

    E-Print Network [OSTI]

    Abdel Rahim, Ma'en Ahmad

    2004-09-30T23:59:59.000Z

    Sequence-specific small interfering RNA (siRNA) duplexes can be used for gene silencing in mammalian cells and as mechanistic probes for determining gene function. Transfection of siRNA for specificity protein 1 (Sp1) in ...

  13. A transfection assay for the biological activity of irradiated PM2 DNA

    E-Print Network [OSTI]

    Newman, Andrew Drennan

    1982-01-01T23:59:59.000Z

    Transfection and Gel Electrophores1s Data for Form I PN2 DNA Exposed to X-Ray Radiation . Transfection and Gel Electrophoresis Data for Form I PN2 DNA Exposed to Ultraviolet Radiation Neasur1ng the Effect of moi of X-Ray Irradiated ONA... of Texas at Dallas Chairman of Advisory Committee: Dr. R. D. Neff A transfection assay was developed to detect radiation-damaged PM2 DNA. By varying growth and incubation condit1ons, the calcium heat shock technique was optimized for PM2 DNA and its...

  14. Linear DNA Low Efficiency Transfection by Liposome Can Be Improved by the Use of Cationic Lipid as Charge Neutralizer

    E-Print Network [OSTI]

    Barbosa, Marcia C. B.

    Linear DNA Low Efficiency Transfection by Liposome Can Be Improved by the Use of Cationic Lipid the efficiency of a liposome-mediated transfection by circular and linear DNA. The results obtained showed a low rate of transfection by linear DNA:liposome complexes. To explore whether the structure

  15. Anionic Nucleotide-Lipids for In Vitro DNA Transfection Salim Khiati,,

    E-Print Network [OSTI]

    Anionic Nucleotide-Lipids for In Vitro DNA Transfection Salim Khiati,, Nathalie Pierre,, Soahary A family of new anionic nucleotide based lipids featuring thymidine-3-monophosphate as nucleotide and 1,2- diacyl-sn-glycerol as lipid moiety for in Vitro delivery of nucleic acids is described. The nucleotide

  16. Transfection of the dextran Alexa Fluor 488 dye into a targeted HeLa cell using Nanofountain Probe

    E-Print Network [OSTI]

    Espinosa, Horacio D.

    transfection is the introduction of molecules, such as nucleic acids or proteins, into a cell to change its channels. The probe has previously been used for high-speed nanopatterning of proteins and nanoparticles to be extremely robust and multi-functional. Researchers have used the NFP-E chip to transfect HeLa cells

  17. Use of a transfected and amplified Drosophila heat shock promoter construction for inducible production of toxic mouse c-myc proteins in CHO cells

    SciTech Connect (OSTI)

    Wurm, F.M.; Gwinn, K.A.; Papoulas, O.; Pallavicini, M.; Kingston, R.E.

    1987-07-24T23:59:59.000Z

    After transfection and selection with methotrexate, CHO cell lines were established which contained up to 2000 copies of an expression vector for c-myc protein. The vector contained the Drosophila heat shock protein 70 (hsp70) promoter fused with the coding region of the mouse c-myc gene. Incubation of cells for up to 3 hours at 43/sup 0/C resulted in at least a 100-fold induction of recombinant c-myc mRNA. When cells were shifted back to 37/sup 0/C, within 1 to 4 hours, this RNA was translated into protein to yield about 250 ..mu..g per 10/sup 9/ cells. Cells died a few hours later, suggesting that high concentrations of intracellular c-myc are cytotoxic. 47 refs., 5 figs.

  18. Flow-Through Microfluidic Device for High-Efficiency Transfection of Mammalian Cells through Combined Microelectroporation and Sonoporation 

    E-Print Network [OSTI]

    Longsine, Whitney Leigh

    2012-07-16T23:59:59.000Z

    than that of electroporation. Importantly, there was little difference in short term cell viability between the three methods (maintained at > 90 percent). The average transfection efficiency for electro-sonoporation was 81.25 percent and cell...

  19. Self-assembled pentablock copolymers for selective and sustained gene delivery

    SciTech Connect (OSTI)

    Zhang, Bingqi

    2011-05-15T23:59:59.000Z

    The poly(diethylaminoethyl methacrylate) (PDEAEM) - Pluronic F127 - PDEAEM pentablock copolymer (PB) gene delivery vector system has been found to possess an inherent selectivity in transfecting cancer cells over non-cancer cells in vitro, without attaching any targeting ligands. In order to understand the mechanism of this selective transfection, three possible intracellular barriers to transfection were investigated in both cancer and non-cancer cells. We concluded that escape from the endocytic pathway served as the primary intracellular barrier for PB-mediated transfection. Most likely, PB vectors were entrapped and rendered non-functional in acidic lysosomes of non-cancer cells, but survived in less acidic lysosomes of cancer cells. The work highlights the importance of identifying intracellular barriers for different gene delivery systems and provides a new paradigm for designing targeting vectors based on intracellular differences between cell types, rather than through the use of targeting ligands. The PB vector was further developed to simultaneously deliver anticancer drugs and genes, which showed a synergistic effect demonstrated by significantly enhanced gene expression in vitro. Due to the thermosensitive gelation behavior, the PB vector packaging both drug and gene was also investigated for its in vitro sustained release properties by using polyethylene glycol diacrylate as a barrier gel to mimic the tumor matrix in vivo. Overall, this work resulted in the development of a gene delivery vector for sustained and selective gene delivery to tumor cells for cancer therapy.

  20. Working with Adenoviral Vectors in Animals This cubicle is currently housing _____________ (animal species) that have been transfected with a

    E-Print Network [OSTI]

    Cui, Yan

    on the protocols must be trained in the hazards of working with the recombinant adenovirus before handling species) that have been transfected with a replication-defective adenovirus. The protocol plastic bags, which are then placed in red biosafety bags for infectious waste incineration 5. All

  1. Working with Lentiviral Vectors in Animals This cubicle is currently housing ___________(animal species) that have been transfected with a replication-

    E-Print Network [OSTI]

    Cui, Yan

    on the protocols must be trained in the hazards of working with the recombinant lentivirus before handling species) that have been transfected with a replication- defective lentivirus. The protocol are then placed in red biosafety bags for infectious waste incineration 5. All surfaces and racks that may

  2. Transfection Mechanisms of Polyplexes, Lipoplexes, and Stealth Liposomes in 51 Integrin Bearing DLD1 Colorectal Cancer Cells

    E-Print Network [OSTI]

    Kokkoli, Efie

    Transfection Mechanisms of Polyplexes, Lipoplexes, and Stealth Liposomes in 51 Integrin Bearing DLD of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, Minnesota 55455, United PEGylated (stealth) nontargeted liposomes or PR_b peptide (targeted to 51 integrin) functionalized stealth

  3. Prarie View RDF

    Energy Savers [EERE]

    PRAIRIE VIEW RDF 2 Prairie View RDF Located at JAAP (approx. 40 miles southwest of Chicago), 223 acres on 455 Acre Parcel Will County Owner; Waste Management, Operator ...

  4. Isolation and characterization of Agouti: a diabetes/obesity related gene

    DOE Patents [OSTI]

    Woychik, Richard P. (Knoxville, TN)

    2000-06-27T23:59:59.000Z

    The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

  5. Isolation and characterization of Agouti: a diabetes/obesity related gene

    DOE Patents [OSTI]

    Woychik, Richard P. (Knoxville, TN)

    1998-01-01T23:59:59.000Z

    The present invention relates to the cloning and expression of the Agouti gene and analogous genes in transformed, transfected and transgenic mice. The present invention provides an animal model for the study of diabetes, obesity and tumors for the testing of potential therapeutic agents. The present invention provides oligonucleotide probes for the detection of the Agouti gene and mutations in the gene. The present invention also relates to the isolation and recombinant production of the Agouti gene product, production of antibodies to the Agouti gene product and their use as diagnostic and therapeutic agents.

  6. Chapter 12 Gene Genealogies Noah A. Rosenberg

    E-Print Network [OSTI]

    Rosenberg, Noah

    Chapter 12 ­ Gene Genealogies Noah A. Rosenberg Program in Molecular and Computational Biology can be viewed as the result of mutations on a scaffold of genetic relationships ­ a gene genealogy, migration, species divergence, and changes in population size, an understanding of gene genealogies

  7. World Views From fragmentation

    E-Print Network [OSTI]

    .......................................................11 2. The Seven Components of a World View...................................................... 20 3. The Unity of the Seven Sub........................................... 25 5. The Purpose of the group `Worldviews

  8. Prolactin receptor and signal transduction to milk protein genes

    SciTech Connect (OSTI)

    Djiane, J.; Daniel, N.; Bignon, C. [Unite d`Endocrinologie Moleculaire, Jouy en Josas (France)] [and others

    1994-06-01T23:59:59.000Z

    After cloning of the mammary gland prolactin (PRL) receptor cDNA, a functional assay was established using co-transfection of PRL receptor cDNA together with a milk protein promoter/chloramphenicol acetyl transferase (CAT) construct in Chinese hamster ovary (CHO) cells. Different mutants of the PRL receptor were tested in this CAT assay to delimit the domains in the receptor necessary for signal transduction to milk protein genes. In CHO cells stably transfected with PRL receptor cDNA, high numbers of PRL receptor are expressed. By metabolic labeling and immunoprecipitation, expressed PRL receptor was identified as a single species of 100 kDa. Using these cells, we analyzed the effects of PRL on intracellular free Ca{sup ++} concentration. PRL stimulates Ca{sup ++} entry and induces secondary Ca{sup ++} mobilization. The entry of Ca{sup ++} is a result of an increase in K{sup +} conductance that hyperpolarizes the membranes. We have also analyzed tyrosine phosphorylation induced by PRL. In CHO cells stably transfected with PRL receptor cDNA, PRL induced a very rapid and transient tyrosine phosphorylation of a 100-kDa protein which is most probably the PRL receptor. The same finding was obtained in mammary membranes after PRL injection to lactating rabbits. Whereas tyrosine kinase inhibitors genistein and lavendustin were without effect, PRL stimulation of milk protein gene promoters was partially inhibited by 2 {mu}M herbimycin in CHO cells co-transfected with PRL receptor cDNA and the {Beta} lactoglobulin CAT construct. Taken together these observations indicate that the cytoplasmic domain of the PRL receptor interacts with one or several tyrosine kinases, which may represent early postreceptor events necessary for PRL signal transduction to milk protein genes. 14 refs., 4 figs.

  9. NEWS & VIEWS Glass dynamics

    E-Print Network [OSTI]

    Weeks, Eric R.

    NEWS & VIEWS Glass dynamics Diverging views on glass transition Gregory B. mc.mckenna@ttu.edu T he glass transition is one of the most intriguing phenomena in the world of soft condensed matter. Despite decades of study, many aspects of the behaviour of glass-forming liquids remain elusive

  10. Interactive 3D Gene Expression Viewer Victor E. Gerth*

    E-Print Network [OSTI]

    Vize, Peter D.

    Interactive 3D Gene Expression Viewer Victor E. Gerth* University of Calgary, Department. The Interactive Gene Expression viewer provides a way to view spatial relationships between different gene expression patterns and anatomic features. Web based 3D enabled technologies such as the Interactive Gene

  11. Overexpression of the human BCL-2 gene product results in growth enhancement of Epstein-Barr virus-immortalized B cells

    SciTech Connect (OSTI)

    Tsujimoto, Yoshihide (Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA))

    1989-03-01T23:59:59.000Z

    The biological activity of the human BCL-2 gene product was analyzed in an Epstein-Barr virus (EBV)-infected human lymphoblastoid B-cell line transfected with BCL-2 sequences driven by the simian virus 40 promoter and enhancer. Overproduction of the BCL-2 protein conferred a selective growth advantage to the EBV-infected B cells as compared with control transfectants in low-serum medium and also after seeding at limiting dilution but did not render the cells tumorigenic in athymic nude mice. This growth enhancement was also seen in cells transfected with the BCL-2 gene with its own promoter juxtaposed to the immunoglobulin heavy chain gene enhancer, which represents the translocated form of the BCL-2 gene observed in follicular lymphomas with the t(14;18) translocation. The growth advantage of EBV-infected B cells overproducing the BCL-2 protein is neither due to the enhanced growth factor production nor due to an enhanced sensitivity of the BCL-2 transfectants to interleukins 1 or 6, although both lymphokines are known to stimulate proliferation of EBV-infected B-cell lines. The growth advantage of EBV-infected B-cell lines. The growth advantage of EBV-infected B cells by overproduction of the BCL-2 protein suggests the direct involvement of the BCL-2 gene product in the pathogenesis of follicular lymphoma.

  12. NEWS AND VIEWS PERSPECTIVE

    E-Print Network [OSTI]

    Mahler, D. Luke

    NEWS AND VIEWS PERSPECTIVE Niche diversification follows key innovation in Antarctic fish radiation Oxford Street, Cambridge MA 02138, USA Antarctic notothenioid fishes provide a fascinating evolu- tionary diversification has occurred repeatedly and in parallel. Keywords: community ecology, fish, macroevolution, phylo

  13. Forward viewing OCT endomicroscopy

    E-Print Network [OSTI]

    Liang, Kaicheng

    2014-01-01T23:59:59.000Z

    A forward viewing fiber optic-based imaging probe device was designed and constructed for use with ultrahigh speed optical coherence tomography in the human gastrointestinal tract. The light source was a MEMS-VCSEL at 1300 ...

  14. Single-cell transfection tool enables added control for biological studies May 21, 2013 Northwestern University researchers have developed a new method for delivering molecu

    E-Print Network [OSTI]

    Espinosa, Horacio D.

    , such as nucleic acids or proteins, into change its properties.) However, because bulk electroporation applies-speed nanopatterning of proteins and nanoparticles for drug delivery studies. The new single-cell transfection to be available in late 2013. The technique is proving to be extremely robust and multi-functional. Researchers

  15. Engineering Aerial view of

    E-Print Network [OSTI]

    Yang, Junfeng

    -neutral Torus 2 Climate Change 4 Combustion and Catalysis Laboratory #12;4 5 1Engineering Revolution 5 #12;6 7Columbia Engineering Plus #12;1 1 2 3 4 5 6 Aerial view of Columbia campus with Columbia Engineering-a liated buildings highlighted in blue Columbia Engineering Plus Engineering Revolution 4

  16. Stereoscopic optical viewing system

    DOE Patents [OSTI]

    Tallman, Clifford S. (Walnut Creek, CA)

    1987-01-01T23:59:59.000Z

    An improved optical system which provides the operator a stereoscopic viewing field and depth of vision, particularly suitable for use in various machines such as electron or laser beam welding and drilling machines. The system features two separate but independently controlled optical viewing assemblies from the eyepiece to a spot directly above the working surface. Each optical assembly comprises a combination of eye pieces, turning prisms, telephoto lenses for providing magnification, achromatic imaging relay lenses and final stage pentagonal turning prisms. Adjustment for variations in distance from the turning prisms to the workpiece, necessitated by varying part sizes and configurations and by the operator's visual accuity, is provided separately for each optical assembly by means of separate manual controls at the operator console or within easy reach of the operator.

  17. Stereoscopic optical viewing system

    DOE Patents [OSTI]

    Tallman, C.S.

    1986-05-02T23:59:59.000Z

    An improved optical system which provides the operator with a stereoscopic viewing field and depth of vision, particularly suitable for use in various machines such as electron or laser beam welding and drilling machines. The system features two separate but independently controlled optical viewing assemblies from the eyepiece to a spot directly above the working surface. Each optical assembly comprises a combination of eye pieces, turning prisms, telephoto lenses for providing magnification, achromatic imaging relay lenses and final stage pentagonal turning prisms. Adjustment for variations in distance from the turning prisms to the workpiece, necessitated by varying part sizes and configurations and by the operator's visual accuity, is provided separately for each optical assembly by means of separate manual controls at the operator console or within easy reach of the operator.

  18. Comparison of gene expression in pre-implantation bovine embryos either injected or transfected with a siRNA targeted against E-cadherin

    E-Print Network [OSTI]

    Hanna, Carol Bailey McCormick

    2009-05-15T23:59:59.000Z

    yield of a single milking (Niemann and Kues 2007; Poirier and Blancho 2008). Several companies have been established which are dedicated to producing pharmed products for commercially available therapeutics. Netherlands based, Pharming, uses...

  19. Multi-view kernel construction

    E-Print Network [OSTI]

    Sa, Virginia R.; Gallagher, Patrick W.; Lewis, Joshua M.; Malave, Vicente L.

    2010-01-01T23:59:59.000Z

    5157-z Multi-view kernel construction Virginia R. de Sa ·multiple different graph construction algorithms. The Ng et

  20. TotalView Training

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » Top InnovativeTopoisomeraseTotalView

  1. Protective laser beam viewing device

    DOE Patents [OSTI]

    Neil, George R.; Jordan, Kevin Carl

    2012-12-18T23:59:59.000Z

    A protective laser beam viewing system or device including a camera selectively sensitive to laser light wavelengths and a viewing screen receiving images from the laser sensitive camera. According to a preferred embodiment of the invention, the camera is worn on the head of the user or incorporated into a goggle-type viewing display so that it is always aimed at the area of viewing interest to the user and the viewing screen is incorporated into a video display worn as goggles over the eyes of the user.

  2. False color viewing device

    DOE Patents [OSTI]

    Kronberg, J.W.

    1991-05-08T23:59:59.000Z

    This invention consists of a viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching, the user`s eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage.

  3. False color viewing device

    DOE Patents [OSTI]

    Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

    1992-01-01T23:59:59.000Z

    A viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching the user's eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage.

  4. False color viewing device

    DOE Patents [OSTI]

    Kronberg, J.W.

    1992-10-20T23:59:59.000Z

    A viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching the user's eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage. 7 figs.

  5. The view from Kiev

    SciTech Connect (OSTI)

    Kiselyov, S.

    1993-11-01T23:59:59.000Z

    This article reports the observations of correspondents for the Bulletin (two Russian journalists, one based in Moscow, the other in Kiev) who investigated the status of the Soviet Union's Black Sea Fleet and Ukraine's status as a non-nuclear-weapons state. After two years of wrangling and two earlier failed settlements, Russian President Boris Yeltsin met with Ukrainian President Leonid Kravchuk at Massandra in Crimea. On September 3, the leaders announced that Russia would buy out Ukraine's interest in the fleet and lease the port at Sevastopol. The Massandra summit was also supposed to settle Ukraine's status as a non-nuclear-weapons state. Described here are the Kiev-based correspondent's views on the Massandra summit (and its major topics), which was to have been called off by the Russian foreign ministry when Ukrainian Prime Minister Leonid Kuchma resigned.

  6. From genes to networks: in systematic points of view

    E-Print Network [OSTI]

    2011-01-01T23:59:59.000Z

    Conference on Bioinformatics and Computational Biology LasConference on Bioinformatics & Computational Biology andon Bioinformatics & Computational Biology: Systems Biology.

  7. A Regulator's View of Cogeneration

    E-Print Network [OSTI]

    Shanaman, S. M.

    1982-01-01T23:59:59.000Z

    of the total national electric generation. In view of the energy requirements of Pennsylvania's industry and the impact of increasing energy costs on employment the Commission directed its technical staff to investigate the potential for industrial cogeneration...

  8. Viewing device for electron-beam equipment

    SciTech Connect (OSTI)

    Nasyrov, R.S.

    1985-06-01T23:59:59.000Z

    Viewing devices are used to observe melting, welding, and so on in vacuum systems, an it is necessary to protect the windows from droplets and vapor. A viewing device for electron-beam equipment is described in which the viewing tube and mounting flange are made as a tubular ball joint enclosed in a steel bellows, which render the viewing device flexible. Bending the viewing tube in the intervals between observations protects the viewing window from sputtering and from drops of molten metal.

  9. Wide field of view telescope

    DOE Patents [OSTI]

    Ackermann, Mark R. (Albuquerque, NM); McGraw, John T. (Placitas, NM); Zimmer, Peter C. (Albuquerque, NM)

    2008-01-15T23:59:59.000Z

    A wide field of view telescope having two concave and two convex reflective surfaces, each with an aspheric surface contour, has a flat focal plane array. Each of the primary, secondary, tertiary, and quaternary reflective surfaces are rotationally symmetric about the optical axis. The combination of the reflective surfaces results in a wide field of view in the range of approximately 3.8.degree. to approximately 6.5.degree.. The length of the telescope along the optical axis is approximately equal to or less than the diameter of the largest of the reflective surfaces.

  10. Input a journal Viewing Journals

    E-Print Network [OSTI]

    Sussex, University of

    Journals Contents: Input a journal Viewing Journals Deleting a journal Entering jnl into different period Problems Input a journal 1 Login to Bluqube 2 Select 3 Enter relevant Doc type To select the number of journals you will processing & the total credit value 6 Click on 7 Enter brief description 8

  11. View

    E-Print Network [OSTI]

    2006-02-28T23:59:59.000Z

    Feb 28, 2006 ... For defining the NSO functions composing the battery, we consider two categories: – randomly generated functions, either defined as the ...

  12. View

    E-Print Network [OSTI]

    2008-07-07T23:59:59.000Z

    a battery of benchmark instances of up to 200 nodes are reported. These seem to be the largest instances that have been solved exactly for this problem.

  13. View

    E-Print Network [OSTI]

    2011-06-21T23:59:59.000Z

    May 14, 2010 ... Generally, even testing whether a matrix is in C? is co-NP-complete [23]. ...... One concrete example is the following: ?. ?. ?. ?. ?. ?. 1 1/3 1/3 ...

  14. View

    E-Print Network [OSTI]

    2013-10-14T23:59:59.000Z

    based on median finding, variable fixing, and secant techniques. Keywords: ..... arithmetic operations, the overall complexity is O(n2). D. The worst case bound in ..... always work on its rated clock speed, we turned force the Linux system to use the ..... dimensionally equidistributed uniform pseudo-random number generator.

  15. View

    E-Print Network [OSTI]

    1910-31-00T23:59:59.000Z

    Sep 3, 2003 ... Landau (TDGL) equation, the Maxwell equations, and an energy equation ... varying currents and magnetic fields generate thermal energy, ...

  16. View

    E-Print Network [OSTI]

    2013-11-05T23:59:59.000Z

    Approach. ?. Selin Damla Ahipasao?glu the date of receipt and acceptance should be .... which has the minimum volume is a natural choice from both theoretical and ... This problem has important applications in statistics and solving this prob- ...... the SDPT3 algorithm using the CVX platform on MATLAB, which is a classic.

  17. View

    E-Print Network [OSTI]

    2010-08-12T23:59:59.000Z

    all real solutions of a given system of polynomial equations. ... A crucial ingredient is a semidefinite characterization of the real radical ideal ...... The options.

  18. View

    E-Print Network [OSTI]

    2004-12-25T23:59:59.000Z

    Let fbe a meromorphic function satisfying condition (1.2), and let rj be a sequence with property (2.5). Then the set S is finite and for some subsequence of ...

  19. View

    E-Print Network [OSTI]

    2010-07-30T23:59:59.000Z

    project of the classification of (simple) nuclear separable C*-algebras [E]. Let C? denote the category of separable C*-algebras and ?-homomorphisms.

  20. View

    E-Print Network [OSTI]

    2006-07-11T23:59:59.000Z

    the classification results of Kirchberg and Phillips using the notion of nuclear absorbing ... A simple purely infinite nuclear separable C*-algebra is called.

  1. View

    E-Print Network [OSTI]

    2008-12-16T23:59:59.000Z

    The aim of this paper is to give a survey of some basic theory of semi-infinite programming. .... operations of addition and multiplication by a scalar. We associate ...

  2. Views of the solar system

    SciTech Connect (OSTI)

    Hamilton, C.

    1995-02-01T23:59:59.000Z

    Views of the Solar System has been created as an educational tour of the solar system. It contains images and information about the Sun, planets, moons, asteroids and comets found within the solar system. The image processing for many of the images was done by the author. This tour uses hypertext to allow space travel by simply clicking on a desired planet. This causes information and images about the planet to appear on screen. While on a planet page, hyperlinks travel to pages about the moons and other relevant available resources. Unusual terms are linked to and defined in the Glossary page. Statistical information of the planets and satellites can be browsed through lists sorted by name, radius and distance. History of Space Exploration contains information about rocket history, early astronauts, space missions, spacecraft and detailed chronology tables of space exploration. The Table of Contents page has links to all of the various pages within Views Of the Solar System.

  3. For current viewing resistor loads

    DOE Patents [OSTI]

    Lyons, Gregory R. (Tijeras, NM); Hass, Jay B. (Lee's Summit, MO)

    2011-04-19T23:59:59.000Z

    The invention comprises a terminal unit for a flat cable comprising a BNC-PCB connector having a pin for electrically contacting one or more conducting elements of a flat cable, and a current viewing resistor having an opening through which the pin extends and having a resistor face that abuts a connector face of the BNC-PCB connector, wherein the device is a terminal unit for the flat cable.

  4. ParaView at NERSC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)IntegratedSpeeding access toTestPhysics LabInterconnection RiskMarch StudyParaView

  5. Chisolm View | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty EditCalifornia:PowerCER.png El CER esDataset Country ChileDialogue,China:Chisolm View

  6. Better Buildings Network View | March 2015 | Department of Energy

    Energy Savers [EERE]

    Better Buildings Network View | March 2015 Better Buildings Network View | March 2015 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's...

  7. The Better Buildings Neighborhood View -- December 2013 | Department...

    Office of Environmental Management (EM)

    The Better Buildings Neighborhood View -- December 2013 The Better Buildings Neighborhood View -- December 2013 The Better Buildings Neighborhood View monthly newsletter from the...

  8. Better Buildings Network View | November 2014 | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    November 2014 More Documents & Publications Better Buildings Network View | July-August 2014 Better Buildings Network View | December 2014 Better Buildings Network View | January...

  9. The Better Buildings Neighborhood View - August 2012 | Department...

    Energy Savers [EERE]

    The Better Buildings Neighborhood View - August 2012 The Better Buildings Neighborhood View - August 2012 The Better Buildings Neighborhood View monthly newsletter from the U.S....

  10. Cosmology: a bird's eye view

    E-Print Network [OSTI]

    Alan A. Coley; Sigbjorn Hervik; Woei Chet Lim

    2006-05-15T23:59:59.000Z

    In this essay we discuss the difference in views of the Universe as seen by two different observers. While one of the observers follows a geodesic congruence defined by the geometry of the cosmological model, the other observer follows the fluid flow lines of a perfect fluid with a linear equation of state. We point out that the information these observers collect regarding the state of the Universe can be radically different; while one observes a non-inflating ever-expanding ever-lasting universe, the other observer can experience a dynamical behaviour reminiscent to that of quintessence or even that of a phantom cosmology leading to a 'big rip' singularity within finite time (but without the need for exotic forms of matter).

  11. Better Buildings Network View | May 2014 | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    May 2014 More Documents & Publications Better Buildings Network View | July-August 2014 Better Buildings Network View | June...

  12. MyRED Mobiles Student Views

    E-Print Network [OSTI]

    Farritor, Shane

    tap on Shopping Cart, then select term. Tap on Class Search to find courses to place in your shoppingMyRED Mobiles Student Views Mar 2014 Page 1 Login/Sign-in Enter your MyRED /TrueYou credentials. Tap on any Term bar to view a schedule for the selected term. Home Screen/Main Menu Class Schedule

  13. Groundwater Modeling in ArcView: by integrating ArcView, MODFLOW and

    E-Print Network [OSTI]

    Sengupta, Raja

    Groundwater Modeling in ArcView: by integrating ArcView, MODFLOW and MODPATH Abstract Modeling. This paper addresses groundwater modeling which is one of the many entities in environmental modeling in ArcView 3.2a. The objective was to create an integrated system where a user could do groundwater

  14. Honors Enrichment Contracts Faculty View Page 1 Honors Enrichment Contracts Faculty View

    E-Print Network [OSTI]

    Honors Enrichment Contracts ­ Faculty View Page 1 Honors Enrichment Contracts ­ Faculty View INITIAL APPROVAL #12;Honors Enrichment Contracts ­ Faculty View Page 2 Summary This document contains information about how to make initial and final decisions on Honors Enrichment Contracts submitted by honors

  15. A view-sequential 3D display

    E-Print Network [OSTI]

    Cossairt, Oliver S. (Oliver Strider), 1978-

    2003-01-01T23:59:59.000Z

    This thesis outlines the various techniques for creating electronic 3D displays and analyzes their commercial potential. The thesis argues for the use of view-sequential techniques in the design of 3D displays based on ...

  16. California's Energy Future - The View to 2050

    E-Print Network [OSTI]

    2011-01-01T23:59:59.000Z

    carbon capture and storage, especially as a technology thatCarbon capture and sequestration CCST California Council on Science and Technologytechnology California’s Energy Future - The View to 2050 becomes available. ? ? Fossil fuel with carbon capture

  17. The new option view of investment

    E-Print Network [OSTI]

    Dixit, Avinash K.

    1995-01-01T23:59:59.000Z

    This paper provides a simple introduction to the new option view of investment. We explain the shortcomings of the orthodox theory, and then outline the basic ideas behind the option framework. Several industry examples ...

  18. JOBAID-VIEWING AN EMPLOYEE MATRIX (SUPERVISOR)

    Broader source: Energy.gov [DOE]

    The purpose of this job aid is to guide supervisor users through the step-by-step process of viewing an employee matrix within SuccessFactors Learning.

  19. Incorporating video into Google Mobile Street View

    E-Print Network [OSTI]

    Wright, Christina (Christina E.)

    2010-01-01T23:59:59.000Z

    Mobile Street View is a compelling application but suffers from significant latency problems, especially in limited bandwidth circumstances. Currently, the application uses static images to display street level information. ...

  20. Jefferson and Hamilton as viewed by historians

    E-Print Network [OSTI]

    Jungmeyer, Paul Edward

    1970-01-01T23:59:59.000Z

    JEFFERSON AND HAMILTON AS VIEHED BY HISTORIANS A Thesis Paul Edward Jungmeyer Submitted to the Graduate College of Texas A&M University in ' partial fulfillment of the requirement for the degree of MASTER OF ARTS August, 1970 Major Subject...: History JEFFERSON AND HAMILTON AS VIEWED BY HISTORIANS A Thesis by Paul Edward Jungmeyer Approved as to style and content by: (Chairman of Committee d i~) (Member) (M er) August, 1970 ABSTRACT JEFFERSON AND HAMILTON AS VIEWED BY HISTORIANS...

  1. axial view inductively: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    View of Induction Reasoning for First-Order Logic Physics Websites Summary: , The Alan Turing Centenary Conference (2012)" 12;A Unified View of Induction Reasoning for First in...

  2. Residential Windows and Window Coverings: A Detailed View of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Residential Windows and Window Coverings: A Detailed View of the Installed Base and User Behavior Residential Windows and Window Coverings: A Detailed View of the Installed Base...

  3. Better Buildings Network View | April 2014 | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    April 2014 More Documents & Publications Better Buildings Network View | December 2014 Better Buildings Residential Network Orientation Better Buildings Network View | November...

  4. Better Buildings Network View | February 2014 | Department of...

    Energy Savers [EERE]

    February 2014 Better Buildings Network View | February 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential...

  5. Better Buildings Network View | January 2015 | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    5 More Documents & Publications Better Buildings Network View | December 2014 Better Buildings Network View | November 2014 Better Buildings Residential Network Orientation...

  6. Better Buildings Network View | June 2014 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    June 2014 Better Buildings Network View | June 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network....

  7. Better Buildings Network View | March 2014 | Department of Energy

    Energy Savers [EERE]

    March 2014 Better Buildings Network View | March 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential...

  8. Better Buildings Network View | December 2014 | Department of...

    Energy Savers [EERE]

    December 2014 Better Buildings Network View | December 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential...

  9. Better Buildings Network View | January 2014 | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Better Buildings Network View | January 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential Network....

  10. Better Buildings Network View | October 2014 | Department of...

    Energy Savers [EERE]

    October 2014 Better Buildings Network View | October 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings Residential...

  11. Better Buildings Network View | February 2015 | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    5 More Documents & Publications Better Buildings Network View | November 2014 Better Buildings Network View | September 2014 Better Buildings Residential Network Case Study:...

  12. Measurements using tangentially viewing bolometers on TFTR

    SciTech Connect (OSTI)

    Bush, C.E.; Schivell, J.; Budny, R.; Ellis R.A. III; Goldston, R.J.; McCune, D.; Medley, S.S.; Scott, S.D.; Towner, H.H.; Wieland, R.M.; and others

    1988-08-01T23:59:59.000Z

    Co- and counter-viewing bolometers aimed along a common tangency chord are being used to study power losses due to charge exchange (CX) of fast ions in neutral beam injection (NBI) heated TFTR plasmas. For unidirectional injection, tangential bolometers oriented to view CX loss of circulating fast ions detect losses from the thermal target plasma (impurity radiation and CX) plus power due to the fast ion CX loss, whereas bolometers oppositely directed measure only the target plasma contribution. The difference between the two signals is a measure of the fast ion CX loss. Additional information is obtained by comparing the tangential bolometer signals with those of perpendicularly viewing bolometer monitors and arrays. The measurements are compared to results of the TRANSP code analysis.

  13. BLUE VIEW VISION INSIGHT! Good news--your vision plan

    E-Print Network [OSTI]

    California at Santa Cruz, University of

    WELCOME TO BLUE VIEW VISION INSIGHT! Good news--your vision plan is flexible and easy to use, your discounts, and much more! Blue View Vision InsightSM University of California Student Health Insurance Plan (UC SHIP) 2013/14 Your Blue View Vision Insight Network Blue View Vision Insight offers you

  14. BLUE VIEW VISION INSIGHT! Good news--your vision plan

    E-Print Network [OSTI]

    Barrett, Jeffrey A.

    WELCOME TO BLUE VIEW VISION INSIGHT! Good news--your vision plan is flexible and easy to use, your discounts, and much more! Blue View Vision InsightSM University of California Student Health Insurance Plan (UC SHIP) 2012/13 Your Blue View Vision Insight Network Blue View Vision Insight offers you

  15. Evolution of Genes and Gene Networks in Filamentous Fungi 

    E-Print Network [OSTI]

    Greenwald, Charles Joaquin

    2011-10-21T23:59:59.000Z

    and Materials ................................................................... 15 Results ............................................................................................ 16 Discussion... .............................................................................. 46 14 Relative expression of conidiation-associated genes ................................. 49 15 Expression profiles for significantly regulated genes and FUNCAT category distributions...

  16. Project Title: Older People's View of

    E-Print Network [OSTI]

    Hickman, Mark

    /AGECONCERN/10/NP Age Concern Canterbury provides support for older people, many of whom face loneliness, lowProject Title: Older People's View of Community Support Bachelor of Arts Internship Company information, support and advocacy, to enable older people in Canterbury to have real choices". (http

  17. CITY OF MOUNTAIN VIEW April 12, 2011 .

    E-Print Network [OSTI]

    Ordinance ordinance? Projected effective date: September 1, 2011 Green building or stand-alone energy Energy Ordinance in Combination with Green Building ordinance? Do minimum energy requirements increase No afterCITY OF MOUNTAIN VIEW April 12, 2011 . CaUfomia Energy Commission Attn: Joe Loyer 1516 Ninth

  18. Friction in full view A. P. Merklea

    E-Print Network [OSTI]

    Marks, Laurence D.

    Friction in full view A. P. Merklea and L. D. Marksb Materials Science and Engineering proposed friction mechanisms explaining the unique tribological properties of graphite. Wear of graphite chemical or struc- tural information from the interface during a friction experi- ment. Examples

  19. INVESTIGATION Retrospective View of North American Potato

    E-Print Network [OSTI]

    Douches, David S.

    INVESTIGATION Retrospective View of North American Potato (Solanum tuberosum L.) Breeding in the 20, Madison, Wisconsin, 53706 ABSTRACT Cultivated potato (Solanum tuberosum L.), a vegetatively propagated explore the effects of potato breeding at the genome level, we used 8303 single-nucleotide polymorphism

  20. Lab 9 LabVIEW and GPIB LabVIEW (National Instruments)

    E-Print Network [OSTI]

    Glashausser, Charles

    Automatic data acquisition DAC 01010 Actuator, Heater... control Power amplifiers LabVIEW GPIB GPIB #12 Toolbar Retain Wire Values Button Step Function Buttons #12;Block Diagram Window Front Panel Window

  1. Microfluidic gene synthesis

    E-Print Network [OSTI]

    Kong, David Sun, 1979-

    2008-01-01T23:59:59.000Z

    The ability to synthesize custom de novo DNA constructs rapidly, accurately, and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications ...

  2. View of software for HEP experiments

    SciTech Connect (OSTI)

    Johnstad, H.; Lebrun, P.; Lessner, E.S.; Montgomery, H.E.

    1986-05-01T23:59:59.000Z

    A view of the software structure typical of a High Energy Physics experiment is given and the availability of general software modules in most of the important regions is discussed. The aim is to provide a framework for discussion of capabilities and inadequecies and thereby define areas where effort should be assigned and perhaps also to serve as a useful source document for the newcomer to High Energy Physics. 74 refs.

  3. Gene prioritization and clustering by multi-view text mining , Leon-Charles Tranchevent1

    E-Print Network [OSTI]

    for Biotechnology Information (NCBI) at the National Library of Medicine (NLM). MEDLINE covers all aspects resource for biomedical text mining applications is the MEDLINE database developed by the National Center of controlled vocabularies and the representation schemes of terms occupy a central role in text mining

  4. METHODOLOGY ARTICLE Open Access Gene prioritization and clustering by multi-view

    E-Print Network [OSTI]

    for Biotechnology Informa- tion (NCBI) at the National Library of Medicine (NLM). MEDLINE covers all aspects resource for biomedical text mining applications is the MEDLINE database devel- oped by the National Center of controlled vocabularies and the representation schemes of terms occupy a cen- tral role in text mining

  5. eSales Interactive Mapping -Viewing and Printing Introduction

    E-Print Network [OSTI]

    eSales Interactive Mapping - Viewing and Printing Introduction Our eSales system includes an integrated interactive map facility. This allows you to view and print maps for all lots on offer. Various

  6. Cellulosic Biofuels: Expert Views on Prospects for Advancement: Supplementary Material

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    Cellulosic Biofuels: Expert Views on Prospects for Advancement: Supplementary Material Erin Baker Keywords: Biofuels; Technology R&D; Uncertainty; Environmental policy 2 #12;1 Introduction This paper contains supplementary material for "Cellulosic Biofuels: Expert Views on Prospects for Advancement

  7. Better Buildings Network View | July-August 2014 | Department...

    Energy Savers [EERE]

    July-August 2014 Better Buildings Network View | July-August 2014 The Better Buildings Network View monthly newsletter from the U.S. Department of Energy's Better Buildings...

  8. Alternative Views on Knowledge: Presentation of Open Learner Models

    E-Print Network [OSTI]

    Bull, Susan

    a learner with a graphical view of their Bayesian learner model. STyLE-OLM [2] works with the learner

  9. Polyelectrolyte-based Nanoparticles for Gene and Protein Delivery

    E-Print Network [OSTI]

    Khondee, Supang

    2011-05-31T23:59:59.000Z

    targeting intercellular cell-adhesion molecule-1 (ICAM-1) was conjugated to TAT peptide using a polyethylene glycol (PEG) spacer. Though the transfection efficiency of TAT polyplexes was reduced by PEGylation, TAT complexes targeting ICAM-1 were able...

  10. Loop Quantum Gravity: An Inside View

    E-Print Network [OSTI]

    Thomas Thiemann

    2006-08-29T23:59:59.000Z

    This is a (relatively) non -- technical summary of the status of the quantum dynamics in Loop Quantum Gravity (LQG). We explain in detail the historical evolution of the subject and why the results obtained so far are non -- trivial. The present text can be viewed in part as a response to an article by Nicolai, Peeters and Zamaklar [hep-th/0501114]. We also explain why certain no go conclusions drawn from a mathematically correct calculation in a recent paper by Helling et al [hep-th/0409182] are physically incorrect.

  11. Better Buildings Network View, March 2015

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: The FutureCommentsEnergy Christopher|for1, 20114, 2013Better NewsNetwork View

  12. TotalView | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron Spin Transition in2, 2003Tool ofTopo II:7.1 7.0 8.04.2 7.6 16.6TotalView

  13. Lake View Geothermal Facility | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf Kilauea Volcano, Hawaii9969995°,ILEDSGP/joinHavasuPalmdaleLake View

  14. View from the Bridge | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your DensityEnergy U.S.-China Electric Vehicle and03/02Report | DepartmentTRUVictor Der About Us VictorView

  15. Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP

    SciTech Connect (OSTI)

    Tsutsui, Motomu [Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Sowa-ku, Nagoya, Aichi 466-8550 (Japan); Hasegawa, Hitoki [The Center for Education in Laboratory Animal Research, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501 (Japan); Adachi, Koichi [Radioisotope Research Center Medical Division, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Sowa-ku, Nagoya, Aichi 466-8550 (Japan); Miyata, Maiko [Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501 (Japan); Huang, Peng [Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Sowa-ku, Nagoya, Aichi 466-8550 (Japan); Ishiguro, Naoki [Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Sowa-ku, Nagoya, Aichi 466-8550 (Japan); Hamaguchi, Michinari [Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Sowa-ku, Nagoya, Aichi 466-8550 (Japan); Iwamoto, Takashi [The Center for Education in Laboratory Animal Research, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501 (Japan); Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501 (Japan)], E-mail: iwamoto@isc.chubu.ac.jp

    2008-08-08T23:59:59.000Z

    Microprocessor, the complex of Drosha and DGCR8, promotes the processing of primary microRNA to precursor microRNA, which is a crucial step for microRNA maturation. So far, no convenient assay systems have been developed for observing this step in vivo. Here we report the establishment of highly sensitive cellular systems where we can visually monitor the function of Microprocessor. During a series of screening of transfectants with fusion genes of the EGFP cDNA and primary microRNA genes, we have obtained certain cell lines where introduction of siRNA against DGCR8 or Drosha strikingly augments GFP signals. In contrast, these cells have not responded to Dicer siRNA; thus they have a unique character that GFP signals should be negatively and specifically correlated to the action of Microprocessor among biogenesis of microRNA. These cell lines can be useful tools for real-time analysis of Microprocessor action in vivo and identifying its novel modulators.

  16. Spectral Clustering Gene Ontology Terms to Group Genes by Function

    E-Print Network [OSTI]

    Zell, Andreas

    Spectral Clustering Gene Ontology Terms to Group Genes by Function Nora Speer, Christian Spieth­12, 2005. c Springer-Verlag Berlin Heidelberg 2005 #12;2 N. Speer, C. Spieth, and A. Zell part-of is-a GO

  17. Differentially Coexpressed Genes

    E-Print Network [OSTI]

    Spang, Rainer

    Fine-tuning #12;Do these pattern exist in real data ? Acute Lymphoblastic Leukemia · About 1/3 of all) Classication, subtype discovery, and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression proling, Cancer Cell, 1(2), 133-43. #12;Differential coexpression in phil+ leukemia norm phil

  18. Short-hairpin RNA-mediated Heat shock protein 90 gene silencing inhibits human breast cancer cell growth in vitro and in vivo

    SciTech Connect (OSTI)

    Zuo, Keqiang [Department of General Surgery, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China)] [Department of General Surgery, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China); Li, Dan [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China)] [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China); Pulli, Benjamin [Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, MA 02114 (United States)] [Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, MA 02114 (United States); Yu, Fei; Cai, Haidong; Yuan, Xueyu [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China)] [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China); Zhang, Xiaoping, E-mail: zxpsibs@163.com [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China)] [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China); Lv, Zhongwei, E-mail: heyixue163@163.com [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China)] [Department of Nuclear Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072 (China)

    2012-05-04T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Hsp90 is over-expressed in human breast cancer. Black-Right-Pointing-Pointer The shRNA-mediated gene silencing of Hsp90 resulted in inhibition of cell growth. Black-Right-Pointing-Pointer Akt and NF-kB were down-regulation after transfection due to Hsp90 silencing. Black-Right-Pointing-Pointer The tumor growth ratio was decline due to Hsp90 silencing. Black-Right-Pointing-Pointer The PCNA expression was down-regulation due to Hsp90 silencing. -- Abstract: Hsp90 interacts with proteins that mediate signaling pathways involved in the regulation of essential processes such as proliferation, cell cycle control, angiogenesis and apoptosis. Hsp90 inhibition is therefore an attractive strategy for blocking abnormal pathways that are crucial for cancer cell growth. In the present study, the role of Hsp90 in human breast cancer MCF-7 cells was examined by stably silencing Hsp90 gene expression with an Hsp90-silencing vector (Hsp90-shRNA). RT-PCR and Western blot analyses showed that Hsp90-shRNA specifically and markedly down-regulated Hsp90 mRNA and protein expression. NF-kB and Akt protein levels were down-regulated in Hsp90-shRNA transfected cells, indicating that Hsp90 knockout caused a reduction of survival factors and induced apoptosis. Treatment with Hsp90-shRNA significantly increased apoptotic cell death and caused cell cycle arrest in the G1/S phase in MCF-7 cells, as shown by flow cytometry. Silencing of Hsp90 also reduced cell viability, as determined by MTT assay. In vivo experiments showed that MCF-7 cells stably transfected with Hsp90-shRNA grew slowly in nude mice as compared with control groups. In summary, the Hsp90-shRNA specifically silenced the Hsp90 gene, and inhibited MCF-7 cell growth in vitro and in vivo. Possible molecular mechanisms underlying the effects of Hsp90-shRNA include the degradation of Hsp90 breast cancer-related client proteins, the inhibition of survival signals and the upregulation of apoptotic pathways. shRNA-mediated interference may have potential therapeutic utility in human breast cancer.

  19. Surface functionalization of inorganic nano-crystals with fibronectin and E-cadherin chimera synergistically accelerates trans-gene delivery into embryonic stem cells

    SciTech Connect (OSTI)

    Kutsuzawa, K. [Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501 (Japan); Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777 (Japan); Chowdhury, E.H. [Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501 (Japan); Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777 (Japan); Nagaoka, M. [Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501 (Japan); Maruyama, K. [Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777 (Japan); Akiyama, Y. [Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777 (Japan); Akaike, T. [Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501 (Japan) and Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777 (Japan)]. E-mail: takaike@bio.titech.ac.jp

    2006-11-24T23:59:59.000Z

    Stem cells holding great promises in regenerative medicine have the potential to be differentiated to a specific cell type through genetic manipulation. However, conventional ways of gene transfer to such progenitor cells suffer from a number of disadvantages particularly involving safety and efficacy issues. Here, we report on the development of a bio-functionalized inorganic nano-carrier of DNA by embedding fibronectin and E-cadherin chimera on the carrier, leading to its high affinity interactions with embryonic stem cell surface and accelerated trans-gene delivery for subsequent expression. While only apatite nano-particles were very inefficient in transfecting embryonic stem cells, fibronectin-anchored particles and to a more significant extent, fibronectin and E-cadherin-Fc-associated particles dramatically enhanced trans-gene delivery with a value notably higher than that of commercially available lipofection system. The involvement of both cell surface integrin and E-cadherin in mediating intracellular localization of the hybrid carrier was verified by blocking integrin binding site with excess free fibronectin and up-regulating both integrin and E-cadherin through PKC activation. Thus, the new establishment of a bio-functional hybrid gene-carrier would promote and facilitate development of stem cell-based therapy in regenerative medicine.

  20. September 2013 Most Viewed Documents for Biology And Medicine...

    Office of Scientific and Technical Information (OSTI)

    September 2013 Most Viewed Documents for Biology And Medicine Science Subject Feed Drug Retention Times Center for Human Reliability Studies (2007) 29 > Oleoresin Capsicum...

  1. ALS Technique Gives Novel View of Lithium Battery Dendrite Growth

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ALS Technique Gives Novel View of Lithium Battery Dendrite Growth Print Lithium-ion batteries, popular in today's electronic devices and electric vehicles, could gain significant...

  2. March 2014 Most Viewed Documents for Biology And Medicine | OSTI...

    Office of Scientific and Technical Information (OSTI)

    March 2014 Most Viewed Documents for Biology And Medicine Science Subject Feed Carbon Dioxide Sequestering Using Microalgal Systems Daniel J. Stepan; Richard E. Shockey; Thomas A....

  3. January 2013 Most Viewed Documents for Power Generation And Distributi...

    Office of Scientific and Technical Information (OSTI)

    January 2013 Most Viewed Documents for Power Generation And Distribution Lessons from Large-Scale Renewable Energy Integration Studies: Preprint Bird, L.; Milligan, M. Small punch...

  4. Newbie question: How to view commercial/residential load profile...

    Open Energy Info (EERE)

    Newbie question: How to view commercialresidential load profile datasets? Home > Groups > Buildings I have downloaded SAM (20112414) and cannot access open the .tar files. Help...

  5. New ALS Technique Gives Nanoscale Views of Complex Systems

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nanoscale Views of Complex Systems Print Studying and identifying molecules at the mesoscale has always been challenging-even the best microscopes and spectrometers have...

  6. Better Buildings Network View | September 2014 | Department of...

    Broader source: Energy.gov (indexed) [DOE]

    Financing Program: On-Bill Financing Brings Lenders and Homeowners On Board Better Buildings Network View | December 2014 On-Bill Financing for Energy Efficiency Improvements...

  7. March 2015 Most Viewed Documents for Biology And Medicine | OSTI...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    March 2015 Most Viewed Documents for Biology And Medicine Measuring dopamine release in the human brain with PET Volkow, N.D. Brookhaven National Lab., Upton, NY (United...

  8. The Better Buildings Neighborhood View - July 2013 | Department...

    Energy Savers [EERE]

    Better Buildings Neighborhood View - October 2012 Better Buildings Neighborhood Program Home Accomplishments History Better Buildings Partners Stories Interviews Videos Contact Us...

  9. Human AZU-1 gene, variants thereof and expressed gene products

    DOE Patents [OSTI]

    Chen, Huei-Mei; Bissell, Mina

    2004-06-22T23:59:59.000Z

    A human AZU-1 gene, mutants, variants and fragments thereof. Protein products encoded by the AZU-1 gene and homologs encoded by the variants of AZU-1 gene acting as tumor suppressors or markers of malignancy progression and tumorigenicity reversion. Identification, isolation and characterization of AZU-1 and AZU-2 genes localized to a tumor suppressive locus at chromosome 10q26, highly expressed in nonmalignant and premalignant cells derived from a human breast tumor progression model. A recombinant full length protein sequences encoded by the AZU-1 gene and nucleotide sequences of AZU-1 and AZU-2 genes and variant and fragments thereof. Monoclonal or polyclonal antibodies specific to AZU-1, AZU-2 encoded protein and to AZU-1, or AZU-2 encoded protein homologs.

  10. Performance viewing and editing in ASSESS Outsider

    SciTech Connect (OSTI)

    Snell, M.K. [Sandia National Labs., Albuquerque, NM (US); Key, B.; Bingham, B. [Science and Engineering Associates, Inc., Albuquerque, NM (US)

    1993-07-01T23:59:59.000Z

    The Analytic System and Software for Evaluation of Safeguards and Security (ASSESS) Facility module records site information in the path elements and areas of an Adversary Sequence Diagram. The ASSESS Outsider evaluation module takes this information and first calculates performance values describing how much detection and delay is assigned at each path element and then uses the performance values to determine most-vulnerable paths. This paper discusses new Outsider capabilities that allow the user to view how elements are being defeated and to modify some of these values in Outsider. Outsider now displays how different path element segments are defeated and contrasts the probability of detection for alternate methods of defeating a door (e.g., the lock or the door face itself). The user can also override element segment delays and detection probabilities directly during analysis in Outsider. These capabilities allow users to compare element performance and to verify correct path element performance for all elements, not just those on the most-vulnerable path as is the case currently. Improvements or reductions in protection can be easily checked without creating a set of new facility files to accomplish it.

  11. A HYPERSPECTRAL VIEW OF THE CRAB NEBULA

    SciTech Connect (OSTI)

    Charlebois, M.; Drissen, L.; Bernier, A.-P. [Departement de Physique, de Genie Physique et d'Optique, Universite Laval, Quebec, Quebec G1V 0A6 (Canada); Grandmont, F. [ABB Bomem Inc., 585 boulevard Charest est, Suite 300, Quebec, Quebec G1K 9H4 (Canada); Binette, L., E-mail: maxime.charlebois.1@ulaval.c, E-mail: ldrissen@phy.ulaval.c, E-mail: anne-pier.bernier.1@ulaval.c [Instituto de AstronomIa, UNAM, Ap.70-264, 04510 Mexico, DF (Mexico)

    2010-05-15T23:59:59.000Z

    We have obtained spatially resolved spectra of the Crab nebula in the spectral ranges 450-520 nm and 650-680 nm, encompassing the H{beta}, [O III] {lambda}4959, {lambda}5007, H{alpha}, [N II] {lambda}6548, {lambda}6584, and [S II] {lambda}6717, {lambda}6731 emission lines, with the imaging Fourier transform spectrometer SpIOMM at the Observatoire du Mont-Megantic's 1.6 m telescope. We first compare our data with published observations obtained either from a Fabry-Perot interferometer or from a long-slit spectrograph. Using a spectral deconvolution technique similar to the one developed by Cadez et al., we identify and resolve multiple emission lines separated by large Doppler shifts and contained within the rapidly expanding filamentary structure of the Crab. This allows us to measure important line ratios, such as [N II]/H{alpha}, [S II]/H{alpha}, and [S II] {lambda}6717 /[S II] {lambda}6731 of individual filaments, providing a new insight on the SE-NW asymmetry in the Crab. From our analysis of the spatial distribution of the electronic density and of the respective shocked versus photoionized gas components, we deduce that the skin-less NW region must have evolved faster than the rest of the nebula. Assuming a very simple expansion model for the ejecta material, our data provide us with a complete tridimensional view of the Crab.

  12. Three Views of a Secret in Relativistic Thermodynamics

    E-Print Network [OSTI]

    Tadas K. Nakamura

    2008-12-19T23:59:59.000Z

    It has been shown three different views in relativistic thermodynamics can be derived from the basic formulation proposed by van Kampen and Israel. The way to decompose energy-momentum into the reversible and irreversible parts is not uniquely determined, and different choices result in different views. The effect of difference in the definition of a finite volume is also considered.

  13. O'Leary outlines policy views at confirmation hearing

    SciTech Connect (OSTI)

    Simpson, J.

    1993-02-15T23:59:59.000Z

    In her confirmation hearing, Secretary O'Leary outlined her views on various controversial issues. Among these were management of Yucca Mountain and the search for a nuclear waste repository, research on alternative fuels, encouragement of the use of renewable energy sources, and her views on intergrated resource planning.

  14. Updatable Process Views for Adapting Large Process Models

    E-Print Network [OSTI]

    Ulm, Universität

    Updatable Process Views for Adapting Large Process Models: The proView Demonstrator Jens Kolb. The increasing adoption of process-aware information sys- tems (PAISs) has resulted in large process model collections. To support users having different perspectives on these processes and related data, a PAIS should

  15. A Gene Scrapbook A Tribute to Gene Loh

    E-Print Network [OSTI]

    A Gene Scrapbook A Tribute to Gene Loh on the Occasion of His Retirement Feb 22, 2003 #12;The Early visited such exotic destinations as Salt Lake City, Utah #12;Happy Retirement!!!! We wish you a happy retirement!!!! Although we are sure that you will not just be spending more time with your grandchildren #12;

  16. April 2013 Most Viewed Documents for Renewable Energy Sources...

    Office of Scientific and Technical Information (OSTI)

    11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 1252 > Seventh Edition Fuel Cell Handbook NETL (2004) 628 > Wet cooling towers: rule-of-thumb design and...

  17. June 2014 Most Viewed Documents for Renewable Energy Sources...

    Office of Scientific and Technical Information (OSTI)

    11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 300 > Seventh Edition Fuel Cell Handbook NETL (2004) 118 > Chapter 17. Engineering cost analysis Higbee,...

  18. GenePRIMP: A GENE PRediction IMprovement Pipeline for Prokaryotic genomes

    SciTech Connect (OSTI)

    Pati, Amrita; Ivanova, Natalia N.; Mikhailova, Natalia; Ovchinnikova, Galina; Hooper, Sean D.; Lykidis, Athanasios; Kyrpides, Nikos C.

    2010-04-01T23:59:59.000Z

    We present 'gene prediction improvement pipeline' (GenePRIMP; http://geneprimp.jgi-psf.org/), a computational process that performs evidence-based evaluation of gene models in prokaryotic genomes and reports anomalies including inconsistent start sites, missed genes and split genes. We found that manual curation of gene models using the anomaly reports generated by GenePRIMP improved their quality, and demonstrate the applicability of GenePRIMP in improving finishing quality and comparing different genome-sequencing and annotation technologies.

  19. Mountain View Electric Association, Inc- Energy Efficiency Credit Program

    Broader source: Energy.gov [DOE]

    Mountain View Electric Association, Inc. (MVEA) and Tri-State Generation and Transmission Association Inc., MVEA’s power supplier, will pay credits to MVEA customers who install qualifying energy...

  20. DOE Zero Ready Home Case Study: Clifton View Homes, Kaltenbach...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    want to go anywhere else," said Clifton. Clifton, founder of Clifton View Homes, offers net zero energy homes and home designs. Clifton has been building high-performance homes...

  1. A Transformational View of Cartography W. R. Tobler

    E-Print Network [OSTI]

    Clarke, Keith

    A Transformational View of Cartography W. R. Tobler ABSTRACT. Cartographic transformations aliases are between points, lines, and areas. Substantive transformations occur in map interpolation transformational grammars, as originally devised for linguistics.2, 4 A similar, though less ambitious, attempt

  2. Sea Oil Field Satellite Monitoring: An Opera3onal View

    E-Print Network [OSTI]

    Kuligowski, Bob

    through oil drilling. It is refined and separated, most easily by boiling In the oil industry, the term "North Sea" o`en includes areasSea Oil Field Satellite Monitoring: An Opera3onal View Maurizio

  3. How can I view submitted materials? | OpenEI Community

    Open Energy Info (EERE)

    view submitted materials? Home > Groups > 2014 Geothermal Case Study Challenge Submitted by NickL on 6 May, 2014 - 07:41 1 answer Points: 0 For various reasons, you may want to...

  4. Plan-view Trajectory Estimation with Dense Stereo Background Models

    E-Print Network [OSTI]

    Darrell, T.

    2001-02-01T23:59:59.000Z

    In a known environment, objects may be tracked in multiple views using a set of back-ground models. Stereo-based models can be illumination-invariant, but often have undefined values which inevitably lead to foreground ...

  5. NEWS & VIEWS synchrotron or helium-lamp studies. But

    E-Print Network [OSTI]

    Loss, Daniel

    NEWS & VIEWS synchrotron or helium-lamp studies. But the low energy of the laser photons raises that of a conventional metal. The effects of projection have led to detailed quantitative insights into the properties

  6. axis view techniques: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8 An Asymmetric, Energetic Type Ic Supernova Viewed Off-Axis, and a Link to Gamma-Ray Bursts Astrophysics (arXiv) Summary: Type Ic supernovae, the explosions following the...

  7. ViewDependent Refinement of Progressive Meshes Hugues Hoppe

    E-Print Network [OSTI]

    California at Berkeley, University of

    to these criteria. The algorithm exploits view coherence, supports frame rate regulation, and is found to require not contribute to the image (Figure 12a). While these faces are typically culled early in the rendering pipeline

  8. View-Dependent Refinement of Progressive Meshes Hugues Hoppe

    E-Print Network [OSTI]

    Cohen, Michael

    to these criteria. The algorithm exploits view coherence, supports frame rate regulation, and is found to require in the rendering pipeline, this processing incurs a cost. Similarly, it is often unnecessary to render faces

  9. June 2014 Most Viewed Documents for Fission And Nuclear Technologies...

    Office of Scientific and Technical Information (OSTI)

    Viewed Documents for Fission And Nuclear Technologies Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (1977) 78 Estimation of gas leak rates through very...

  10. Challenges in Applying Formal Methods An SME View

    E-Print Network [OSTI]

    Southampton, University of

    Challenges in Applying Formal Methods An SME View Mathieu Clabaut Systerel, Aix-en-Provence, France classical B and event B to design safety related systems in an SME. 1 Activities Systerel is an SME doing

  11. A Narrower Spectrum for a Wider View of Matter

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wider View of Matter July 9, 2014 Bookmark and Share Ultra-high-resolution dispersive optics of the new inelastic x-ray scattering (IXS) spectrometer (top) and IXS spectrum of...

  12. A Unified View on Speeded Categorization Hongbin Gu 1

    E-Print Network [OSTI]

    Ji, Chuanshu

    A Unified View on Speeded Categorization Hongbin Gu 1 Chuanshu Ji 2 1 Introduction The research of Psychiatry, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA; hongbin

  13. Development of AeroView: an interactive flow diagnostics laboratory

    E-Print Network [OSTI]

    Galls, Samuel Fernando

    1996-01-01T23:59:59.000Z

    This research includes the development of a set of experimental flow-diagnostics techniques for low speed aerodynamics applications and an interactive software for flow field data acquisition and presentation called AeroView. The data collection...

  14. Host nutrition and infectious disease: an ecological view

    E-Print Network [OSTI]

    Smith, Val H.; Jones II, Tyrees P.; Smith, Marilyn S.

    2005-06-01T23:59:59.000Z

    Nutrition is typically discussed in terms of maintaining a proper diet and avoiding nutrient deficiency diseases. However, nutrition can also be viewed from an ecological standpoint: mammalian hosts and their pathogens ...

  15. Evolution of Genes and Gene Networks in Filamentous Fungi

    E-Print Network [OSTI]

    Greenwald, Charles Joaquin

    2011-10-21T23:59:59.000Z

    ........................................................................... 62 1 CHAPTER I INTRODUCTION The Pezizomycotina, a subphylum within the phylum Ascomycota, commonly known as the filamentous fungi, are a diverse group of important organisms. There are over 60,000 species representing between 210... in a secondary metabolite gene cluster responsible for roles in the loline biosynthetic pathway of Epichlo? and Neotyphodium species. Two genes lolC and lolD were of interest because: 1) lolines are unique to these closely related species; and 8 2...

  16. Gene encoding herbicide safener binding protein

    DOE Patents [OSTI]

    Walton, Jonathan D. (East Lansing, MI); Scott-Craig, John S. (East Lansing, MI)

    1999-01-01T23:59:59.000Z

    The cDNA encoding safener binding protein (SafBP), also referred to as SBP1, is set forth in FIG. 5 and SEQ ID No. 1. The deduced amino acid sequence is provided in FIG. 5 and SEQ ID No. 2. Methods of making and using SBP1 and SafBP to alter a plant's sensitivity to certain herbicides or a plant's responsiveness to certain safeners are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors and seeds from said plants.

  17. Statistical mechanics of gene competition 

    E-Print Network [OSTI]

    Venegas-Ortiz, Juan; Ortiz, Juan Venegas

    2013-11-28T23:59:59.000Z

    Statistical mechanics has been applied to a wide range of systems in physics, biology, medicine and even anthropology. This theory has been recently used to model the complex biochemical processes of gene expression and ...

  18. Nonviral Vectors for Gene Delivery

    E-Print Network [OSTI]

    Baoum, Abdulgader Ahmed

    2011-04-26T23:59:59.000Z

    optimized for gene delivery by varying particle surface chemistry using different coating materials that adsorb to the particle surface during formation. A variety of cationic coating materials were studied and compared to more conventional surfactants used...

  19. Biogas From Municipal WWTPs: Fuel Cells Viewed as a Value Proposition...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    From Municipal WWTPs: Fuel Cells Viewed as a Value Proposition Biogas From Municipal WWTPs: Fuel Cells Viewed as a Value Proposition Presentation about the value proposition for...

  20. View from the Bridge: Commercial Vehicle Perspective | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your DensityEnergy U.S.-China Electric Vehicle and03/02Report | DepartmentTRUVictor Der About Us VictorViewView

  1. Metazoan Gene Families from Metazome

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Metazome is a joint project of the Department of Energy's Joint Genome Institute and the Center for Integrative Genomics to facilitate comparative genomic studies amongst metazoans. Clusters of orthologous and paralogous genes that represent the modern descendents of ancestral gene sets are constructed at key phylogenetic nodes. These clusters allow easy access to clade specific orthology/paralogy relationships as well as clade specific genes and gene expansions. As of version 2.0.4, Metazome provides access to twenty-four sequenced and annotated metazoan genomes, clustered at nine evolutionarily significant nodes. Where possible, each gene has been annotated with PFAM, KOG, KEGG, and PANTHER assignments, and publicly available annotations from RefSeq, UniProt, Ensembl, and JGI are hyper-linked and searchable. The included organisms (by common name) are: Human, Mouse, Rat, Dog, Opossum, Chicken, Frog, Stickleback, Medaka, Fugu pufferfish; Zebrafish, Seasquirt - savignyi, Seasquirt - intestinalis, Amphioxus, Sea Urchin, Fruitfly, Mosquite, Yellow Fever Mosquito, Silkworm, Red Flour Beetle, Worm, Briggsae Worm, Owl limpet (snail), and Sea anemone. [Copied from Metazome Overview at http://www.metazome.net/Metazome_info.php

  2. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    E-Print Network [OSTI]

    Hoen, Ben

    2010-01-01T23:59:59.000Z

    Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices *

  3. Gene encoding plant asparagine synthetase

    DOE Patents [OSTI]

    Coruzzi, Gloria M. (New York, NY); Tsai, Fong-Ying (New York, NY)

    1993-10-26T23:59:59.000Z

    The identification and cloning of the gene(s) for plant asparagine synthetase (AS), an important enzyme involved in the formation of asparagine, a major nitrogen transport compound of higher plants is described. Expression vectors constructed with the AS coding sequence may be utilized to produce plant AS; to engineer herbicide resistant plants, salt/drought tolerant plants or pathogen resistant plants; as a dominant selectable marker; or to select for novel herbicides or compounds useful as agents that synchronize plant cells in culture. The promoter for plant AS, which directs high levels of gene expression and is induced in an organ specific manner and by darkness, is also described. The AS promoter may be used to direct the expression of heterologous coding sequences in appropriate hosts.

  4. In all cases, ALOX5AP and its synonyms were highlighted automati-cally in each view. To highlight disease terms in these views, we

    E-Print Network [OSTI]

    Cai, Long

    the results easy to explore. The novo|seek search box interface allows the user to enter a term (or the production of substances that cause inflammation, such as leukotrienes1. When we entered ALOX5AP as a search a simple view (title, journal and author), a snippet view (a simple view and the search term with flanking

  5. View Management for Driver Assistance in an HMD

    E-Print Network [OSTI]

    cases. Nevertheless, an increasing number of companies have started to develop light- weight of (currently handheld) mobile augmented reality applications. For cars, this setup seems equally plausible factors need to be considered by future application developers. In view of this question we performed

  6. Analyzing and Visualizing Cosmological Simulations with ParaView

    SciTech Connect (OSTI)

    Woodring, Jonathan [Los Alamos National Laboratory (LANL); Heitmann, Katrin [ORNL; Ahrens, James P [ORNL; Fasel, Patricia [Los Alamos National Laboratory (LANL); Hsu, Chung-Hsing [ORNL; Habib, Salman [ORNL; Pope, Adrian [Los Alamos National Laboratory (LANL)

    2011-01-01T23:59:59.000Z

    The advent of large cosmological sky surveys - ushering in the era of precision cosmology - has been accompanied by ever larger cosmological simulations. The analysis of these simulations, which currently encompass tens of billions of particles and up to a trillion particles in the near future, is often as daunting as carrying out the simulations in the first place. Therefore, the development of very efficient analysis tools combining qualitative and quantitative capabilities is a matter of some urgency. In this paper, we introduce new analysis features implemented within ParaView, a fully parallel, open-source visualization toolkit, to analyze large N-body simulations. A major aspect of ParaView is that it can live and operate on the same machines and utilize the same parallel power as the simulation codes themselves. In addition, data movement is in a serious bottleneck now and will become even more of an issue in the future; an interactive visualization and analysis tool that can handle data in situ is fast becoming essential. The new features in ParaView include particle readers and a very efficient halo finder that identifies friends-of-friends halos and determines common halo properties, including spherical overdensity properties. In combination with many other functionalities already existing within ParaView, such as histogram routines or interfaces to programming languages like Python, this enhanced version enables fast, interactive, and convenient analyses of large cosmological simulations. In addition, development paths are available for future extensions.

  7. Viewable image size Max view area (W x H)

    E-Print Network [OSTI]

    Saskatchewan, University of

    standards Environmental Preset resolutions Presets / user settings Viewing adjustments Rotating screen768 WXGA+: 1440x900 1600x900 WSXGA+: 1680x1050 Standard presets: VGA/DisplayPort: 26; DVI-D: 25; user 90 degrees from landscape to portrait (requires image rotation support from graphics controller

  8. Viewable image size Max view area (W x H)

    E-Print Network [OSTI]

    Saskatchewan, University of

    standards Environmental Preset resolutions Presets / user settings Viewing adjustments Rotating screen: 1920x1200 Standard presets: 30; user settings: 20 Tilt: 30° back, 0° forward; lift: 4.3" (110mm rotation support from graphics controller) On screen display with digital user controls (8 languages

  9. Self-Calibration from Two Views Richard I. Hartley

    E-Print Network [OSTI]

    Hartley, Richard

    Self-Calibration from Two Views Richard I. Hartley G.E. CRD, Schenectady, NY, 12301. Email : hartley@crd.ge.com Abstract A new practical method is given for the self-calibration of a camera and calibration is computed from an analysis of point matches between the two images. The method requires

  10. Self-Calibration from Multiple Views with a Rotating Camera

    E-Print Network [OSTI]

    Hartley, Richard

    Self-Calibration from Multiple Views with a Rotating Camera Richard I. Hartley G.E. CRD, Schenectady, NY, 12301. Email : hartley@crd.ge.com Abstract. A new practical method is given for the self-calibration orientations of the camera and calibration is computed from an analysis of point matches between the images

  11. adaptive optics views: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    optics views First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Design of an Adaptive Scanning Optical...

  12. General view on nuclear fission Karl-Heinz Schmidt

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    General view on nuclear fission Karl-Heinz Schmidt Beatriz Jurado CENBG, CNRS/IN2 P3 Chemin du.amouroux@cea.fr 1 in2p3-00976648,version1-10Apr2014 #12;Abstract: A general approach to nuclear fission is described which ex- plains the complex appearance of fission observables by universal principles of theoretical

  13. Learning the Topology of Object Views Jan Wieghardt1

    E-Print Network [OSTI]

    Würtz, Rolf P.

    Malsburg2,3 1 SIEMENS AG, CT SE 1, Otto-Hahn-Ring 6, D-81730 M¨unchen, Germany jan.wieghardt@mchp.siemens such a representation by deriving transformations between the different views of a given object, so that they can be pa, we construct such a representation by deriving transformations between the different visual

  14. Home Timeslide View Flipcard Voice RecognitionApplication

    E-Print Network [OSTI]

    Sóbester, András

    Home Timeslide View Flipcard Voice RecognitionApplication Solar DefectAnalysis Search N O V E M B E EnergyPanels 4. The Future In Technology 5. Hands On Solar Training 6. Wind EnergySolutions ads by media R 1 0 , 2 0 Revolutionary ultrasonic nozzle can reduce water and energy used for cleaning by ten

  15. The view-based approach to dynamic inter-organizational

    E-Print Network [OSTI]

    Dustdar, Schahram

    The view-based approach to dynamic inter-organizational workflow cooperation Issam Chebbi, Samir Distributed Systems Group This paper presents a novel approach to inter-organizational workflow co- operation of workflows and their resources, thus providing powerful ways for inter- organizational workflow configuration

  16. Lithium Hexamethyldisilazide: A View of Lithium Ion Solvation

    E-Print Network [OSTI]

    Collum, David B.

    Lithium Hexamethyldisilazide: A View of Lithium Ion Solvation through a Glass-Bottom Boat BRETT L and reactivities, we were drawn to lithium hexamethyldisilazide (LiHMDS; (Me3Si)2NLi) by its promi- nence principles of lithium ion coordination chemistry.2 Understanding how solvation influences organolithium

  17. www.advmat.de www.MaterialsViews.com

    E-Print Network [OSTI]

    Hong, Soon Hyung

    by plasma- enhanced chemical vapor deposition. Methane (CH4) was used as the carbon source to synthesize. The strong protein-based adhesives found in marine mussel Mytilus edulis, provide an important insight for the solidification by oxidative chemical crosslinking.[21­24] From a chemical point of view, covalent crosslinking

  18. How species interact Altering the Standard View on Trophic Ecology

    E-Print Network [OSTI]

    Canet, Léonie

    How species interact Altering the Standard View on Trophic Ecology Roger Arditi and Lev R. Ginzburg, the French National Institute for Agricultural Research, in the research unit of Ecology and Evolution of ecology and evolution at Stony Brook University since 1977. He has published widely on theoretical

  19. View-Based Query Processing: On the Relationship Between Rewriting,

    E-Print Network [OSTI]

    Calvanese, Diego

    amounts to computing the tuples satisfying the query in all databases consistent with the views. Rewriting in the setting of semistructured databases, and in particular for the basic query class in this setting, i-0311326, IIS-9908435, IIS-9978135, EIA-0086264, and ANI-0216467, by US-Israel BSF grant 9800096, by Texas

  20. The Once and Future Warm Earth: A Paleoceanographic View

    E-Print Network [OSTI]

    Sukop, Mike

    The Once and Future Warm Earth: A Paleoceanographic View Dr. Ted C. Moore, Jr., University of Michigan Predictions of climatic warming in the near future, if true, will approximate the very warm--and ended. In the early and mid Eocene the Arctic Ocean was ice-free and subtropical flora and fauna were

  1. news and views nucleotide binds to the polymerase and

    E-Print Network [OSTI]

    Schedl, Paul

    news and views nucleotide binds to the polymerase and before it is incorporated into DNA. Recent structures of several structurally diverse DNA polymerases complexed to DNA and nucleotide substrates have shown that their active sites adopt a closed conforma- tion upon binding to the correct nucleotide12

  2. Multiple Field of View MR Fluoroscopy Pelin Aksit,1

    E-Print Network [OSTI]

    Atalar, Ergin

    Multiple Field of View MR Fluoroscopy Pelin Aksit,1 J. Andrew Derbyshire,2 Jean-Michel Serfaty,1 by several investigators (1­ 8). Intravascular MR compares favorably with other clini- cal diagnostic methods and Ergin Atalar1* This work describes a real-time imaging and visualization tech- nique that allows

  3. NEWS & VIEWS X-RaY oPtiCS

    E-Print Network [OSTI]

    Loss, Daniel

    NEWS & VIEWS X-RaY oPtiCS Clarity through a keyhole Janos Hajdu1,2 & Filipe R. n. C. maia2 are at 1 Stanford Synchrotron Radiation Laboratory, Stanford Linear Accelerator Center, 2575 Sand Hill Road, Menlo, as it does in conventional crystallography, but only on the wavelength used, the extent of ionization damage

  4. APOLLO OVER THE MOON A View From Orbit

    E-Print Network [OSTI]

    Rathbun, Julie A.

    #12;s Near ride #12;APOLLO OVER THE MOON A View From Orbit #12;#12;NASA SP-362 APOLLO OVER THE MOO Catalogingin Publication Data Main entry under title: Apollo over the moon. (NASA SP ;362) Bibliography: p. 251. 1. Moon-Photographs from space. 2. Project Apollo. I. Masursky, Harold, 1922- 11. Colton, George

  5. An Integrated View of Security Analysis and Performance Evaluation

    E-Print Network [OSTI]

    Aldini, Alessandro

    An Integrated View of Security Analysis and Performance Evaluation: Trading QoS with Covert Channel@sti.uniurb.it Abstract. Security analysis and performance evaluation are two funda- mental activities in the system. This is accomplished by proposing a tool-supported methodology that combines noninterference-based security analysis

  6. GenePRIMP: Improving Microbial Gene Prediction Quality

    SciTech Connect (OSTI)

    Pati, Amrita [DOE Joint Genome Institute's Genome Biology group

    2009-05-29T23:59:59.000Z

    Amrita Pati of the DOE Joint Genome Institute's Genome Biology group talks about a computational pipeline that evaluates the accuracy of gene models in genomes and metagenomes at different stages of finishing at the "Sequencing, Finishing, Analysis in the Future" meeting in Santa Fe, NM

  7. Essential Genes Are More Evolutionarily Conserved Than Are Nonessential Genes

    E-Print Network [OSTI]

    Jordan, King

    Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA Jordan, Igor B. Rogozin, Yuri I. Wolf, and Eugene V. Koonin1 National Center for Biotechnology functionally dispensable and/or redundant than are nonessential genes. Given the role of pu- rifying selection

  8. The cAMP Response Element Binding protein (CREB) is activated by Insulin-like Growth Factor-1 (IGF-1) and regulates myostatin gene expression in skeletal myoblast

    SciTech Connect (OSTI)

    Zuloaga, R. [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile)] [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile); Fuentes, E.N.; Molina, A. [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile) [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile); Interdisciplinary Center for Aquaculture Research (INCAR), Víctor Lamas 1290, PO Box 160-C, Concepción (Chile); Valdés, J.A., E-mail: jvaldes@unab.cl [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile); Interdisciplinary Center for Aquaculture Research (INCAR), Víctor Lamas 1290, PO Box 160-C, Concepción (Chile)

    2013-10-18T23:59:59.000Z

    Highlights: •IGF-1 induces the activation of CREB via IGF-1R/PI3K/PLC signaling pathway. •Calcium dependent signaling pathways regulate myostatin gene expression. •IGF-1 regulates myostatin gene expression via CREB transcription in skeletal myoblast. -- Abstract: Myostatin, a member of the Transforming Growth Factor beta (TGF-?) superfamily, plays an important role as a negative regulator of skeletal muscle growth and differentiation. We have previously reported that IGF-1 induces a transient myostatin mRNA expression, through the activation of the Nuclear Factor of Activated T cells (NFAT) in an IP{sub 3}/calcium-dependent manner. Here we examined the activation of CREB transcription factor as downstream targets of IGF-1 during myoblast differentiation and its role as a regulator of myostatin gene expression. In cultured skeletal myoblast, IGF-1 induced the phosphorylation and transcriptional activation of CREB via IGF-1 Receptor/Phosphatidylinositol 3-Kinase (PI3K)/Phospholipase C gamma (PLC ?), signaling pathways. Also, IGF-1 induced calcium-dependent molecules such as Calmodulin Kinase II (CaMK II), Extracellular signal-regulated Kinases (ERK), Protein Kinase C (PKC). Additionally, we examined myostatin mRNA levels and myostatin promoter activity in differentiated myoblasts stimulated with IGF-1. We found a significant increase in mRNA contents of myostatin and its reporter activity after treatment with IGF-1. The expression of myostatin in differentiated myoblast was downregulated by the transfection of siRNA–CREB and by pharmacological inhibitors of the signaling pathways involved in CREB activation. By using pharmacological and genetic approaches together these data demonstrate that IGF-1 regulates the myostatin gene expression via CREB transcription factor during muscle cell differentiation.

  9. ascl1 target genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for postgenomic analysis of mammalian gene function is gene targeting McConnell, Susan 2 Infrared lasermediated gene induction in targeted Chemistry Websites Summary: a heat shock...

  10. abolishes gene targeting: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for postgenomic analysis of mammalian gene function is gene targeting McConnell, Susan 2 Infrared lasermediated gene induction in targeted Chemistry Websites Summary: a heat shock...

  11. ancestral gene relationships: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    thought previously of entire genomes, rather than individual genes, on the basis of the analysis of gene orders. Human, mouse Batzoglou, Serafim 17 Analyzing gene relationships...

  12. 20Engineering Gene Circuits: Foundations

    E-Print Network [OSTI]

    You, Lingchong

    ................................................................. 20-363 20.2 Designing Gene Circuits............................................. 20-364 Modeling characterized as ``robustness.'' Based on extensive studies over the last several decades, much engineered switches [11­14], oscillators [15,16], logic gates [17­19], metabolic control [20], reengineered

  13. Regulation of the genes involved in nitrification.

    SciTech Connect (OSTI)

    Arp, D.J.; Sayavedra-Soto, L.A.

    2003-08-14T23:59:59.000Z

    OAK-B135 This project focuses on the characterization of the regulation of the genes involved in nitrification in the bacterium Nitrosomonas europaea. The key genes in the nitrification pathway, amo and hao, are present in multiple copies in the genome. The promoters for these genes were identified and characterized. It was shown that there were some differences in the transcriptional regulation of the copies of these genes.

  14. Plant nitrogen regulatory P-PII genes

    DOE Patents [OSTI]

    Coruzzi, Gloria M. (New York, NY); Lam, Hon-Ming (Hong Kong, HK); Hsieh, Ming-Hsiun (Woodside, NY)

    2001-01-01T23:59:59.000Z

    The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the

  15. Uses of antimicrobial genes from microbial genome

    DOE Patents [OSTI]

    Sorek, Rotem; Rubin, Edward M.

    2013-08-20T23:59:59.000Z

    We describe a method for mining microbial genomes to discover antimicrobial genes and proteins having broad spectrum of activity. Also described are antimicrobial genes and their expression products from various microbial genomes that were found using this method. The products of such genes can be used as antimicrobial agents or as tools for molecular biology.

  16. INVESTIGATION Gene Genealogies Within a Fixed Pedigree,

    E-Print Network [OSTI]

    INVESTIGATION Gene Genealogies Within a Fixed Pedigree, and the Robustness of Kingman's Coalescent, not as a random quantity. Gene genealogical models should describe the outcome of the percolation of genetic provide a surprisingly accurate description of gene genealogies on a fixed pedigree. We study

  17. Short Specialist Review Gene structure prediction

    E-Print Network [OSTI]

    Brendel, Volker

    Short Specialist Review Gene structure prediction in plant genomes Volker Brendel Iowa State) within most genes makes the problem of computational gene structure prediction distinct from (and harder prediction in vertebrates. The second reason is pragmatic. Expressed Sequence Tag (EST) sequencing and whole

  18. Inferring Gene Family Histories in Yeast Identifies Lineage Specific Expansions

    E-Print Network [OSTI]

    Ames, Ryan M.; Money, Daniel; Lovell, Simon C.

    2014-06-12T23:59:59.000Z

    The complement of genes found in the genome is a balance between gene gain and gene loss. Knowledge of the specific genes that are gained and lost over evolutionary time allows an understanding of the evolution of biological ...

  19. Integrating Ontological Knowledge and Textual Evidence in Estimating Gene and Gene Product Similarity

    SciTech Connect (OSTI)

    Sanfilippo, Antonio P.; Posse, Christian; Gopalan, Banu; Tratz, Stephen C.; Gregory, Michelle L.

    2006-06-08T23:59:59.000Z

    With the rising influence of the Gene On-tology, new approaches have emerged where the similarity between genes or gene products is obtained by comparing Gene Ontology code annotations associ-ated with them. So far, these approaches have solely relied on the knowledge en-coded in the Gene Ontology and the gene annotations associated with the Gene On-tology database. The goal of this paper is to demonstrate that improvements to these approaches can be obtained by integrating textual evidence extracted from relevant biomedical literature.

  20. Thermostable cellulase from a thermomonospora gene

    DOE Patents [OSTI]

    Wilson, D.B.; Walker, L.P.; Zhang, S.

    1997-10-14T23:59:59.000Z

    The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity. 3 figs.

  1. Method for determining gene knockouts

    DOE Patents [OSTI]

    Maranas, Costas D. (Port Matilda, PA); Burgard, Anthony R. (State College, PA); Pharkya, Priti (State College, PA)

    2011-09-27T23:59:59.000Z

    A method for determining candidates for gene deletions and additions using a model of a metabolic network associated with an organism, the model includes a plurality of metabolic reactions defining metabolite relationships, the method includes selecting a bioengineering objective for the organism, selecting at least one cellular objective, forming an optimization problem that couples the at least one cellular objective with the bioengineering objective, and solving the optimization problem to yield at least one candidate.

  2. Method for determining gene knockouts

    DOE Patents [OSTI]

    Maranas, Costa D; Burgard, Anthony R; Pharkya, Priti

    2013-06-04T23:59:59.000Z

    A method for determining candidates for gene deletions and additions using a model of a metabolic network associated with an organism, the model includes a plurality of metabolic reactions defining metabolite relationships, the method includes selecting a bioengineering objective for the organism, selecting at least one cellular objective, forming an optimization problem that couples the at least one cellular objective with the bioengineering objective, and solving the optimization problem to yield at least one candidate.

  3. Apolipoprotein gene involved in lipid metabolism

    DOE Patents [OSTI]

    Rubin, Edward (Berkeley, CA); Pennacchio, Len A. (Sebastopol, CA)

    2007-07-03T23:59:59.000Z

    Methods and materials for studying the effects of a newly identified human gene, APOAV, and the corresponding mouse gene apoAV. The sequences of the genes are given, and transgenic animals which either contain the gene or have the endogenous gene knocked out are described. In addition, single nucleotide polymorphisms (SNPs) in the gene are described and characterized. It is demonstrated that certain SNPs are associated with diseases involving lipids and triglycerides and other metabolic diseases. These SNPs may be used alone or with SNPs from other genes to study individual risk factors. Methods for intervention in lipid diseases, including the screening of drugs to treat lipid-related or diabetic diseases are also disclosed.

  4. Nepal: Western Views of the Maoist Insurgency and the Royal Takeover

    E-Print Network [OSTI]

    Burleigh, Peter

    2005-01-01T23:59:59.000Z

    Governance Presentation No. 2 Nepal: Western Views of theworkingpapers/index.html NEPAL: Western Views of the Maoistof the Maoist revolt in Nepal in 1996, there have been a

  5. Travel, work, and telecommunications: a view of the electronics revolution and its potential impacts

    E-Print Network [OSTI]

    Garrison, William L.; Deakin, Elizabeth

    2006-01-01T23:59:59.000Z

    TELECOMMUNICATIONS: A VIEW OF THE ELECTRONICS REVOLUTION ANDtechnologies and other electronics innovations ontelecommunications and other electronics innovations on the

  6. View Maintenance in a Warehousing Environment Yue Zhuge, Hector Garcia-Molina, Joachim Hammer, Jennifer Widom

    E-Print Network [OSTI]

    Hammer, Joachim

    information, avail- able for queries and analysis (e.g., decision support, or data mining) IK93]. As relevantView Maintenance in a Warehousing Environment Yue Zhuge, Hector Garcia-Molina, Joachim Hammer are updated. This view maintenance prob- lem di ers from the traditional one in that the view de nition

  7. GenomeView: a next-generation genome browser Thomas Abeel1,2,3,

    E-Print Network [OSTI]

    Gent, Universiteit

    GenomeView: a next-generation genome browser Thomas Abeel1,2,3, *, Thomas Van Parys1,2 , Yvan Saeys GenomeView, a stand-alone genome browser specifically designed to visualize and manipulate a multitude of genomics data. GenomeView enables users to dynamically browse high volumes of aligned short-read data

  8. Ground control failures. A pictorial view of case studies

    SciTech Connect (OSTI)

    Peng, S.S.

    2007-07-01T23:59:59.000Z

    The book shows, in pictorial views, many forms and/or stages of types of failures in mines, for instance, cutter, roof falls, and cribs. In each case, the year of occurrence is stated in the beginning so that the environment or technological background under which it occurred are reflected. The narrative than begins with the mining and geological conditions, followed by a description of the ground control problems and recommended solutions and results, if any. The sections cover failure of pillars, roof falls, longwall, roof bolting, multiple-seam mining, floor heave, longwall, flooding and weathering of coal, old workings, and shortwall and thin-seam plow longwall.

  9. Prairie View A&M University Whole Campus Energy Analysis

    E-Print Network [OSTI]

    Haberl, J. S.; Claridge, D. E.; Turner, W. D.

    1991-01-01T23:59:59.000Z

    the guaranteed-savings energy management program at the Prairie View Campus. The two primary findings are as follows: (1) Savings to date. The current (April 1990) calculated guaranteed savings equal or exceed the monthly contracted savings rate. Electricity... consumption has decreased sharply since April 1987. Natural gas usage, and electric demand have also decreased significantly. The savings began to appear in October of 1987 at a rate of about $5,000 per month. These savings grew at a steady pace for the next...

  10. Chevy Chase View, Maryland: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand JumpConceptual Model, click here.Telluric SurveyChelanVermont: EnergyView, Maryland: Energy Resources

  11. Skyline View, Pennsylvania: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro Industries Pvt LtdShawangunk, New York:SiG SolarSkykomish, Washington: Energy ResourcesView,

  12. A View from the Bridge - DOE Perspective | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China 2015ofDepartmentDepartment of Energy ThisThisthe National EnergyLookBroad view

  13. LabVIEW Core 2 Course | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5 - -/e),,s -LabgrantsLab team makesLab'sLabVIEW

  14. Prairie View Gas Recovery Biomass Facility | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal PwerPerkins County, Nebraska: EnergyPiratiniEdwards,PoseyPoudrePowers EnergyCityPrairieRose,View

  15. Logan View Public Schools Wind Project | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOf KilaueaInformation Other AlternativePark,CedarPowerView Public

  16. Mountain View, California: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup |JilinLu anMicrogreenMoon LakeMountain Electric Coop, IncView,

  17. OpenEI:Neutral point of view | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup |JilinLuOpenNorthOlympia Green FuelsperCivicVersionNeutral point of view

  18. Better Buildings Network View | April 2014 | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742Energy China U.S. Department ofJune 2, 2015Energyon23264Compare energy use byNew1Network View

  19. Bay View, Ohio: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:EzfeedflagBiomass Conversions IncBay County, Florida: Energy Resources JumpHill,250986°,View,

  20. A View from the Bridge | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: The Future of1 A Strategic Framework for SMRA View from the Bridge - DOEThis

  1. A View from the Bridge | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: The Future of1 A Strategic Framework for SMRA View from the Bridge - DOEThisOn-

  2. A View from the Bridge | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: The Future of1 A Strategic Framework for SMRA View from the Bridge -

  3. Forest View, Illinois: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdf Jump to:ar-80m.pdfFillmoreGabbs ValleyCity, Florida:Oklahoma:Forest View,

  4. Valley View, Ohio: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere IRaghuraji Agro IndustriesTown of Ladoga,planning methodologies andVacant Jump669°,Hill, NorthPark,View,

  5. Imaging gene expression in real-time using aptamers

    SciTech Connect (OSTI)

    Shin, Il Chung [Ames Laboratory

    2012-11-02T23:59:59.000Z

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging microscopy). Real-time transcription was measured by FLIM-FRET, which was detected by the decrease in donor lifetime resulting from ligand binding to IMAGEtags that were newly synthesized from the activated GAL1 promoter. The FRET signal was specific for transcribed IMAGEtags.

  6. Imaging gene expression in real-time using aptamers

    SciTech Connect (OSTI)

    Shin, Il Chung

    2011-12-13T23:59:59.000Z

    Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging microscopy). Real-time transcription was measured by FLIM-FRET, which was detected by the decrease in donor lifetime resulting from ligand binding to IMAGEtags that were newly synthesized from the activated GAL1 promoter. The FRET signal was specific for transcribed IMAGEtags.

  7. Methods for monitoring multiple gene expression

    SciTech Connect (OSTI)

    Berka, Randy (Davis, CA); Bachkirova, Elena (Davis, CA); Rey, Michael (Davis, CA)

    2012-05-01T23:59:59.000Z

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  8. Methods for monitoring multiple gene expression

    DOE Patents [OSTI]

    Berka, Randy; Bachkirova, Elena; Rey, Michael

    2013-10-01T23:59:59.000Z

    The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

  9. Gene expression abnormalities in the autistic brain

    E-Print Network [OSTI]

    Chow, Maggie Lok Mun

    2011-01-01T23:59:59.000Z

    lymphoblastoid cell lines (LCLs) derived from individualsby profiling gene expression in LCLs derived from livingsubjects. LCLs derived from (1) three monozygotic twin pairs

  10. THE JOURNAL OF GENE MEDICINE RESEARCH ARTICLE J Gene Med 2005; 7: 898907.

    E-Print Network [OSTI]

    Hemminki, Akseli

    THE JOURNAL OF GENE MEDICINE RESEARCH ARTICLE J Gene Med 2005; 7: 898­907. Published online 9 March Revised: 19 November 2004 Accepted: 24 November 2004 Abstract Background VP22 is a herpes simplex virus

  11. OpenGeneX:AToolkitforOpenSourceGeneExpressionInformatics Project Description Overview

    E-Print Network [OSTI]

    Weller, Jennifer Walsh

    interface (API), allowing them to interface specialized existing software to OpenGeneX, such as interactive standards by providing freely available reference implementations. A. Lessons from GeneXTM As stated above

  12. Statistical Measure of a Gene Evolution The Case of Glyceraldehyde-3-Phosphate Dehydrogenase Gene

    E-Print Network [OSTI]

    Chattopadhyay, S; Chakrabarti, J; Chattopadhyay, Sujay; Sahoo, Satyabrata; Chakrabarti, Jayprokas

    2000-01-01T23:59:59.000Z

    The enzyme Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) catalyses the decomposition of glucose. The gene that produces the GAPDH is therefore present in a wide class of organisms. We show that for this gene the average value of the fluctuations in nucleotide distribution in the codons, normalized to strand bias, provides a reasonable measure of how the gene has evolved in time.

  13. From Biophysics to Evolutionary Genetics: Statistical Aspects of Gene Regulation

    E-Print Network [OSTI]

    Lässig, Michael

    From Biophysics to Evolutionary Genetics: Statistical Aspects of Gene Regulation Michael L.e., if the protein produced from it is present in the cell. Genes interact by regulation: the protein of one gene can influence the production of protein from another gene. Gene regulation can take place during transcription

  14. High resolution ion Doppler spectroscopy at Prairie View Rotamak

    SciTech Connect (OSTI)

    Houshmandyar, Saeid; Yang Xiaokang [Solar Observatory Department, Prairie View A and M University, Prairie View, Texas 77446 (United States); Magee, Richard [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States)

    2012-10-15T23:59:59.000Z

    A fast ion Doppler spectroscopy (IDS) diagnostic system is installed on the Prairie View Rotamak to measure ion temperature and plasma flow. The diagnostic employs a single channel photomultiplier tube and a Jarrell-Ash 50 monochromator with a diffraction grating line density of 1180 lines/mm, which allows for first order spectra of 200-600 nm. The motorized gear of the monochromator allows spectral resolution of 0.01 nm. Equal IDS measurements are observed for various impurity emission lines of which carbon lines exhibit stronger intensities. Furthermore, the diagnostics is examined in an experiment where plasma experiences sudden disruption and quick recovery. In this case, the IDS measurements show {approx}130% increase in ion temperature. Flow measurements are shown to be consistent with plasma rotation.

  15. Gene coding for the E1 endoglucanase

    DOE Patents [OSTI]

    Thomas, S.R.; Laymon, R.A.; Himmel, M.E.

    1996-07-16T23:59:59.000Z

    The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol. 6 figs.

  16. WHAT IS A GENE? DEPARTMENT OF GENETICS,

    E-Print Network [OSTI]

    Batzoglou, Serafim

    . Studies of the effect of x-ray radiation and of unstable genes are the best means now availableWHAT IS A GENE? M. DEMEREC DEPARTMENT OF GENETICS, CARNEGIE INSTITUTION OF WASHINGTON, COLD SPRING Scholarly Publishing Project http://www.esp.org The original work, upon which this electronic edition

  17. Computational Diagnostics based on Large Scale Gene

    E-Print Network [OSTI]

    Spang, Rainer

    Computational Diagnostics based on Large Scale Gene Expression Profiles using MCMC Rainer Spang = Data Loadings Singular values Expression levels of super genes, orthogonal matrix #12;)( genessuperall- #12;Given the Few Profiles With Known Diagnosis: · The uncertainty on the right model is high

  18. Nucleotide Frequency Variation Across Human Genes

    E-Print Network [OSTI]

    Majewski, Jacek

    Nucleotide Frequency Variation Across Human Genes Elizabeth Louie, Jurg Ott, and Jacek Majewski1 The Rockefeller University, New York, New York 10021, USA The frequencies of individual nucleotides exhibit significant fluctuations across eukaryotic genes. In this paper, we investigate nucleotide variation across

  19. Assignment of Orthologous Genes via Genome Rearrangement

    E-Print Network [OSTI]

    Lonardi, Stefano

    Assignment of Orthologous Genes via Genome Rearrangement Xin Chen, Jie Zheng, Zheng Fu, Peng Nan of genomes is a fundamental and challenging problem in comparative genomics. Existing methods that assign sequence similarity and evolutionary events at a genome level, where orthologous genes are assumed

  20. Tissue-specific gene silencing monitored in circulating RNA

    E-Print Network [OSTI]

    Sehgal, Alfica

    Pharmacologic target gene modulation is the primary objective for RNA antagonist strategies and gene therapy. Here we show that mRNAs encoding tissue-specific gene transcripts can be detected in biological fluids and that ...

  1. Interacting models of cooperative gene regulation Debopriya Das*, Nilanjana Banerjee*

    E-Print Network [OSTI]

    Interacting models of cooperative gene regulation Debopriya Das*, Nilanjana Banerjee* , and Michael, especially mammals, where cooperative control of gene regulation is absolutely essential. cooperativity control in gene regulation networks. It requires cooperative binding of multiple transcription factors

  2. apoptosis related gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of protein Barkai, Naama 10 Inference of Gene Relations from Microarray Data by Abduction Computer Technologies and Information Sciences Websites Summary: Inference of Gene...

  3. apoptosis related genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of protein Barkai, Naama 10 Inference of Gene Relations from Microarray Data by Abduction Computer Technologies and Information Sciences Websites Summary: Inference of Gene...

  4. albumin gene expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biology, and multiple orthogonal gene switches are needed Zhao, Huimin 223 Metabolic load and heterologous gene expression CiteSeer Summary: The expression of a foreign...

  5. at1 receptor gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    28 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  6. angiotensin receptor gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    36 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  7. arthritis candidate genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    37 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  8. Organization and evolution of the rat tyrosine hydroxylase gene

    SciTech Connect (OSTI)

    Brown, E.R.; Coker, G.T. III; O'Malley, K.L.

    1987-08-11T23:59:59.000Z

    This report describes the organization of the rat tyrosine hydroxylase (TH) gene and compares its structure with the human phenylalanine hydroxylase gene. Both genes are single copy and contain 13 exons separated by 12 introns. Remarkably, the positions of 10 out 12 intron/exon boundaries are identical for the two genes. These results support the idea that these hydroxylases genes are members of a gene family which has a common evolutionary origin. The authors predict that this ancestral gene would have encoded exons similar to those of TH prior to evolutionary drift to other members of this gene family.

  9. The specificity and evolution of gene regulatory elements

    E-Print Network [OSTI]

    Friedman, Robin Carl

    2010-01-01T23:59:59.000Z

    The regulation of gene expression underlies the morphological, physiological, and functional differences between human cell types, developmental stages, and healthy and disease states. Gene regulation in eukaryotes is ...

  10. agnostic gene coding: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    25 Next Page Last Page Topic Index 21 Associating Genes with Gene Ontology Codes Using a Maximum Entropy Analysis of Biology and Medicine Websites Summary: and select appropriate...

  11. BESC Submits 32 Gene Disclosures for Patents | ORNL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    invention disclosures for 32 different genes that can help improve the yield of ethanol from cellulosic biomass. These genes or their variants function to overcome...

  12. Site Welcome Rebecca | My A Single-Cell Transfection Tool

    E-Print Network [OSTI]

    Espinosa, Horacio D.

    is the introduction of molecules, such as nucleic acids or proteins, into a cell to change its properties.) However has previously been used for high-speed nanopatterning of proteins and nanoparticles for drug delivery to be available in late 2013. The technique is proving to be extremely robust and multi-functional. Researchers

  13. augment transfection efficiency: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    patients with severely restricted peripheral Peli, Eli 96 RESEARCH ARTICLE Open Access SMART: scalable and modular augmented reality Engineering Websites Summary: RESEARCH...

  14. agents enhanced transfection: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    under the mission of energy saving and reduction of CO2, in particular car-pool and car-sharing management policies. In order to achieve the object of decision analysis for...

  15. Non-coplanar polychlorinated biphenyls (PCBs) are direct agonists for the human pregnane-X receptor and constitutive androstane receptor, and activate target gene expression in a tissue-specific manner

    SciTech Connect (OSTI)

    Al-Salman, Fadheela; Plant, Nick, E-mail: N.Plant@Surrey.ac.uk

    2012-08-15T23:59:59.000Z

    The polychlorinated biphenyl group possesses high environmental persistence, leading to bioaccumulation and a number of adverse effects in mammals. Whilst coplanar PCBs elicit their toxic effects through agonism of the aryl hydrocarbon receptor; however, non-coplanar PCBs are not ligands for AhR, but may be ligands for members of the nuclear receptor family of proteins. To better understand the biological actions of non-coplanar PCBs, we have undertaken a systematic analysis of their ability to activate PXR and CAR-mediated effects. Cells were exposed to a range of non-coplanar PCBs (99, 138, 153, 180 and 194), or the coplanar PCB77: Direct activation of PXR and CAR was measured using a mammalian receptor activation assay in human liver cells, with rifampicin and CITCO used as positive controls ligands for PXR and CAR, respectively; activation of target gene expression was examined using reporter gene plasmids for CYP3A4 and MDR1 transfected into liver, intestine and lung cell lines. Several of the non-coplanar PCBs directly activated PXR and CAR, whilst the coplanar PCB77 did not. Non-coplanar PCBs were also able to activate PXR/CAR target gene expression in a substitution- and tissue-specific manner. Non-coplanar PCBs act as direct activators for the nuclear receptors PXR and CAR, and are able to elicit transcriptional activation of target genes in a substitution- and tissue-dependent manner. Chronic activation of PXR/CAR is linked to adverse effects and must be included in any risk assessment of PCBs. -- Highlights: ? Several Non-coplanar PCBs are able to directly activate both PXR and CAR in vitro. ? PCB153 is the most potent direct activator of PXR and CAR nuclear receptors. ? Non-coplanar PCB activation of CYP3A4/MDR1 reporter genes is structure-dependent. ? Non-coplanar PCB activate CYP3A4/MDR1 reporter genes in a tissue-dependent. ? PCB153 is the most potent activator of PXR/CAR target gene in all tissues.

  16. Report of Energy Efficiency Study and Metering/Utilities Profile for Electricity Deregulation at Prairie View A&M University (PVAMU) Prairie View, Texas

    E-Print Network [OSTI]

    Zhu, Y.; Claridge, D. E.; Giebler, T.; Abushakra, B.; Turner, W. D.

    1999-01-01T23:59:59.000Z

    The physical plant director and staff at Prairie View A&M University (PVAMU) do a good job of maintaining PVAMU facilities and keeping expenses down. During our visit, however, we were able to identify several opportunities for improving energy...

  17. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31T23:59:59.000Z

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  18. Viewing spin structures with soft x-ray microscopy

    SciTech Connect (OSTI)

    Fischer, Peter

    2010-06-01T23:59:59.000Z

    The spin of the electron and its associated magnetic moment marks the basic unit for magnetic properties of matter. Magnetism, in particular ferromagnetism and antiferromagnetism is described by a collective order of these spins, where the interaction between individual spins reflects a competition between exchange, anisotropy and dipolar energy terms. As a result the energetically favored ground state of a ferromagnetic system is a rather complex spin configuration, the magnetic domain structure. Magnetism is one of the eldest scientific phenomena, yet it is one of the most powerful and versatile utilized physical effects in modern technologies, such as in magnetic storage and sensor devices. To achieve highest storage density, the relevant length scales, such as the bit size in disk drives is now approaching the nanoscale and as such further developments have to deal with nanoscience phenomena. Advanced characterization tools are required to fully understand the underlying physical principles. Magnetic microscopes using polarized soft X-rays offer a close-up view into magnetism with unique features, these include elemental sensitivity due to X-ray magnetic dichroism effects as contrast mechanism, high spatial resolution provided by state-of-the-art X-ray optics and fast time resolution limited by the inherent time structure of current X-ray sources, which will be overcome with the introduction of ultrafast and high brilliant X-ray sources.

  19. Einstein, incompleteness, and the epistemic view of quantum states

    E-Print Network [OSTI]

    Nicholas Harrigan; Robert W. Spekkens

    2007-06-18T23:59:59.000Z

    Does the quantum state represent reality or our knowledge of reality? In making this distinction precise, we are led to a novel classification of hidden variable models of quantum theory. Indeed, representatives of each class can be found among existing constructions for two-dimensional Hilbert spaces. Our approach also provides a fruitful new perspective on arguments for the nonlocality and incompleteness of quantum theory. Specifically, we show that for models wherein the quantum state has the status of something real, the failure of locality can be established through an argument considerably more straightforward than Bell's theorem. The historical significance of this result becomes evident when one recognizes that the same reasoning is present in Einstein's preferred argument for incompleteness, which dates back to 1935. This fact suggests that Einstein was seeking not just any completion of quantum theory, but one wherein quantum states are solely representative of our knowledge. Our hypothesis is supported by an analysis of Einstein's attempts to clarify his views on quantum theory and the circumstance of his otherwise puzzling abandonment of an even simpler argument for incompleteness from 1927.

  20. $?$-Ray Pulsars: Emission Zones and Viewing Geometries, A Computer Animation

    E-Print Network [OSTI]

    I. -A. Yadigaroglu; Roger W. Romani

    1994-06-08T23:59:59.000Z

    The computer animation illustrates the geometries described in a paper by the same authors. The preprint is available as number 9401045. The opening scene shows dipole field lines emanating from the polar caps of a rotating neutron star. The dipole axis is inclined along the green rods. The field lines shown are defined from the condition that they be tangent to the light cylinder (the cylindrical radius at which the tangential velocity of rotation reaches the speed of light). The static dipole field lines are smoothly morphed into the correct retarted-potential vacuum solutions. A red surface spanning these field lines is painted. In the next scene the blue surfaces represent the outer gaps above the surface of last closed field lines. High energy emission (blue) is produced in these outer gaps, and is beamed tangentially along the field lines. The radio emission (green) originates close to the surface of the star and is beamed along the dipole axes. The inclination angle $\\alpha$ of the dipole and the viewing angle $\\zeta$ are chosen to match the Crab parameters; $\\alpha$ = 70, $\\zeta$ = 65. The corresponding light curve is computed and shown for these angles, and the red dot traces rotation phase. The next scene shows the situation for angles appropriate to PSR1706-44; $\\alpha$ = 45, $\\zeta$ = 65. The final scene is a possibility for Geminga; $\\alpha$ = 20, $\\zeta$ = 75. These angles are poorly constrained as there is no radio emission.

  1. Synchrotron Radiation Therapy from a Medical Physics point of view

    SciTech Connect (OSTI)

    Prezado, Y.; Berkvens, P.; Braeuer-Krisch, E.; Renier, M.; Bravin, A. [ID17 Biomedical Beamline, European Synchrotron Radiation Facility (ESRF), 6 Rue Jules Horowitz B.P. 220, 38043 Grenoble Cedex (France); Adam, J. F. [INSERM, U836, Equipe 6, BP 170, Grenoble Cedex 9, F-38042 (France); Universite Joseph Fourier, B.P. 51, Grenoble Cedex 9, F-38041 (France); ID17 Biomedical Beamline, European Synchrotron Radiation Facility (ESRF), 6 Rue Jules Horowitz B.P. 220, 38043 Grenoble Cedex (France); Centre Hospitalier Universitaire, B.P. 217, Grenoble Cedex 9, F-38043 (France); Martinez-Rovira, I. [ID17 Biomedical Beamline, European Synchrotron Radiation Facility (ESRF), 6 Rue Jules Horowitz B.P. 220, 38043 Grenoble Cedex (France); Institut de Tecniques Energetiques, Universitat Politecnica de Catalunya, Diagonal 647, E-08028 Barcelona (Spain); Fois, G. [ID17 Biomedical Beamline, European Synchrotron Radiation Facility (ESRF), 6 Rue Jules Horowitz B.P. 220, 38043 Grenoble Cedex (France); Dipartimento di Fisica, Universita degli Studi di Cagliari, Strada Provinciale Monserrato Sestu km 0.700, Monserrato, Cagliari 09042 (Italy); Thengumpallil, S. [ID17 Biomedical Beamline, European Synchrotron Radiation Facility (ESRF), 6 Rue Jules Horowitz B.P. 220, 38043 Grenoble Cedex (France); Dipartimento di Fisica E. Amaldi, Universita degli Studi Roma Tre, 84 Via della Vasca Navale, 00146 Roma (Italy); Edouard, M.; Deman, P. [INSERM, U836, Equipe 6, BP 170, Grenoble Cedex 9, F-38042 (France); Universite Joseph Fourier, B.P. 51, Grenoble Cedex 9, F-38041 (France); ID17 Biomedical Beamline, European Synchrotron Radiation Facility (ESRF), 6 Rue Jules Horowitz B.P. 220, 38043 Grenoble Cedex (France); Vautrin, M. [INSERM, U836, Equipe 6, BP 170, Grenoble Cedex 9, F-38042 (France); Universite Joseph Fourier, B.P. 51, Grenoble Cedex 9, F-38041 (France); DOSIsoft, Cachan (France)

    2010-07-23T23:59:59.000Z

    Synchrotron radiation (SR) therapy is a promising alternative to treat brain tumors, whose management is limited due to the high morbidity of the surrounding healthy tissues. Several approaches are being explored by using SR at the European Synchrotron Radiation Facility (ESRF), where three techniques are under development Synchrotron Stereotactic Radiation Therapy (SSRT), Microbeam Radiation Therapy (MRT) and Minibeam Radiation Therapy (MBRT).The sucess of the preclinical studies on SSRT and MRT has paved the way to clinical trials currently in preparation at the ESRF. With this aim, different dosimetric aspects from both theoretical and experimental points of view have been assessed. In particular, the definition of safe irradiation protocols, the beam energy providing the best balance between tumor treatment and healthy tissue sparing in MRT and MBRT, the special dosimetric considerations for small field dosimetry, etc will be described. In addition, for the clinical trials, the definition of appropiate dosimetry protocols for patients according to the well established European Medical Physics recommendations will be discussed. Finally, the state of the art of the MBRT technical developments at the ESRF will be presented. In 2006 A. Dilmanian and collaborators proposed the use of thicker microbeams (0.36-0.68 mm). This new type of radiotherapy is the most recently implemented technique at the ESRF and it has been called MBRT. The main advantage of MBRT with respect to MRT is that it does not require high dose rates. Therefore it can be more easily applied and extended outside synchrotron sources in the future.

  2. Distance matters: the impact of gene proximity in bacterial gene regulation

    E-Print Network [OSTI]

    Otto Pulkkinen; Ralf Metzler

    2013-05-13T23:59:59.000Z

    Following recent discoveries of colocalization of downstream-regulating genes in living cells, the impact of the spatial distance between such genes on the kinetics of gene product formation is increasingly recognized. We here show from analytical and numerical analysis that the distance between a transcription factor (TF) gene and its target gene drastically affects the speed and reliability of transcriptional regulation in bacterial cells. For an explicit model system we develop a general theory for the interactions between a TF and a transcription unit. The observed variations in regulation efficiency are linked to the magnitude of the variation of the TF concentration peaks as a function of the binding site distance from the signal source. Our results support the role of rapid binding site search for gene colocalization and emphasize the role of local concentration differences.

  3. Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats

    SciTech Connect (OSTI)

    Marín-Prida, Javier [Centre for Research and Biological Evaluations (CEIEB), Institute of Pharmacy and Food, University of Havana, Ave. 23 e/ 214 y 222, La Lisa, PO Box: 430, Havana (Cuba); Pavón-Fuentes, Nancy [International Centre for Neurological Restoration (CIREN), Ave. 25 e/ 158 y 160, Playa, PO Box: 11300, Havana (Cuba); Llópiz-Arzuaga, Alexey; Fernández-Massó, Julio R. [Centre for Genetic Engineering and Biotechnology (CIGB), Ave. 31 e/158 y 190, Playa, PO Box: 6162, Havana (Cuba); Delgado-Roche, Liván [Centre for Research and Biological Evaluations (CEIEB), Institute of Pharmacy and Food, University of Havana, Ave. 23 e/ 214 y 222, La Lisa, PO Box: 430, Havana (Cuba); Mendoza-Marí, Yssel; Santana, Seydi Pedroso; Cruz-Ramírez, Alieski; Valenzuela-Silva, Carmen; Nazábal-Gálvez, Marcelo; Cintado-Benítez, Alberto [Centre for Genetic Engineering and Biotechnology (CIGB), Ave. 31 e/158 y 190, Playa, PO Box: 6162, Havana (Cuba); Pardo-Andreu, Gilberto L. [Centre for Research and Biological Evaluations (CEIEB), Institute of Pharmacy and Food, University of Havana, Ave. 23 e/ 214 y 222, La Lisa, PO Box: 430, Havana (Cuba); Polentarutti, Nadia [Istituto Clinico Humanitas (IRCCS), Rozzano (Italy); Riva, Federica [Department of Veterinary Science and Public Health (DIVET), University of Milano (Italy); Pentón-Arias, Eduardo [Centre for Genetic Engineering and Biotechnology (CIGB), Ave. 31 e/158 y 190, Playa, PO Box: 6162, Havana (Cuba); Pentón-Rol, Giselle [Centre for Genetic Engineering and Biotechnology (CIGB), Ave. 31 e/158 y 190, Playa, PO Box: 6162, Havana (Cuba)

    2013-10-01T23:59:59.000Z

    Since the inflammatory response and oxidative stress are involved in the stroke cascade, we evaluated here the effects of Phycocyanobilin (PCB, the C-Phycocyanin linked tetrapyrrole) on PC12 cell survival, the gene expression and the oxidative status of hypoperfused rat brain. After the permanent bilateral common carotid arteries occlusion (BCCAo), the animals were treated with saline or PCB, taking samples 24 h post-surgery. Global gene expression was analyzed with GeneChip Rat Gene ST 1.1 from Affymetrix; the expression of particular genes was assessed by the Fast SYBR Green RT-PCR Master Mix and Bioplex methods; and redox markers (MDA, PP, CAT, SOD) were evaluated spectrophotometrically. The PCB treatment prevented the H{sub 2}O{sub 2} and glutamate induced PC12 cell injury assessed by the MTT assay, and modulated 190 genes (93 up- and 97 down-regulated) associated to several immunological and inflammatory processes in BCCAo rats. Furthermore, PCB positively modulated 19 genes mostly related to a detrimental pro-inflammatory environment and counteracted the oxidative imbalance in the treated BCCAo animals. Our results support the view of an effective influence of PCB on major inflammatory mediators in acute cerebral hypoperfusion. These results suggest that PCB has a potential to be a treatment for ischemic stroke for which further studies are needed. - Highlights: • Phycocyanobilin (PCB) prevents H{sub 2}O{sub 2} and glutamate induced PC12 cell viability loss. • Anterior cortex and striatum are highly vulnerable to cerebral hypoperfusion (CH). • PCB modulates 190 genes associated to inflammation in acute CH. • PCB regulates 19 genes mostly related to a detrimental pro-inflammatory environment. • PCB restores redox and immune balances showing promise as potential stroke therapy.

  4. Gene expression profiles in irradiated cancer cells

    SciTech Connect (OSTI)

    Minafra, L.; Bravatà, V.; Russo, G.; Ripamonti, M.; Gilardi, M. C. [IBFM CNR - LATO, Cefalù, Segrate (Italy)] [IBFM CNR - LATO, Cefalù, Segrate (Italy)

    2013-07-26T23:59:59.000Z

    Knowledge of the molecular and genetic mechanisms underlying cellular response to radiation may provide new avenues to develop innovative predictive tests of radiosensitivity of tumours and normal tissues and to improve individual therapy. Nowadays very few studies describe molecular changes induced by hadrontherapy treatments, therefore this field has to be explored and clarified. High-throughput methodologies, such as DNA microarray, allow us to analyse mRNA expression of thousands of genes simultaneously in order to discover new genes and pathways as targets of response to hadrontherapy. Our aim is to elucidate the molecular networks involved in the sensitivity/resistance of cancer cell lines subjected to hadrontherapy treatments with a genomewide approach by using cDNA microarray technology to identify gene expression profiles and candidate genes responsible of differential cellular responses.

  5. Parallel gene synthesis in a microfluidic device

    E-Print Network [OSTI]

    Kong, David S.

    The ability to synthesize custom de novo DNA constructs rapidly, accurately and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications ...

  6. Predicting gene function from images of cells

    E-Print Network [OSTI]

    Jones, Thouis Raymond, 1971-

    2007-01-01T23:59:59.000Z

    This dissertation shows that biologically meaningful predictions can be made by analyzing images of cells. In particular, groups of related genes and their biological functions can be predicted using images from large ...

  7. Characterization of a unique embedded gene

    E-Print Network [OSTI]

    Zhang, Ning

    1999-01-01T23:59:59.000Z

    ). Plasmids were either transformed into host cells or stored in ? 20'C as water solutions. The oligonucleotides were purchased from Gene Technologies Laboratory (GTL) in the Department of Biology at Texas A&M University (TAMU). In all experiments involving...

  8. Control of gene expression by cell size

    E-Print Network [OSTI]

    Wu, Chia-Yung

    2010-01-01T23:59:59.000Z

    Polyploidy, increased copy number of whole chromosome sets in the genome, is a common cellular state in evolution, development and disease. Polyploidy enlarges cell size and alters gene expression, producing novel phenotypes ...

  9. INVESTIGATION Coding Gene Single Nucleotide Polymorphism

    E-Print Network [OSTI]

    Bernatchez, Louis

    INVESTIGATION Coding Gene Single Nucleotide Polymorphism Mapping and Quantitative Trait Loci QTL detection single nucleotide polymorphisms (SNP) reproduction Salvelinus fontinalis Linkages maps such as microsatellites and single nucleotide polymorphisms (SNPs). Also, compared with previ- ous methods based

  10. Characterization of the Bovine Cathelicidin Gene Family

    E-Print Network [OSTI]

    Flores, Erin Gillenwaters

    2012-10-19T23:59:59.000Z

    genome assembly (Btau_4.0) confirmed current gene order. Comparative sequence analysis for 10 domestic cattle breeds representing both Bos taurus taurus and Bos taurus indicus revealed 60 SNPs, 7 of which were nonsynonymous, and 5 indel mutations. Data...

  11. Gene-environment interactions in genetic epidemiology

    E-Print Network [OSTI]

    Spinka, Christine Marie

    2005-02-17T23:59:59.000Z

    joint linkage disequilibrium and linkage mapping of quantitative trait loci is developed. Subsequently, methods are needed to determine the interaction, if any, between these genes and environmental risk factors. Many of these factors, such as weight... . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 Mapping Quantitative Trait Loci Using Complex Pedigrees 2 1.3 Gene-environment Interactions . . . . . . . . . . . . . . . 3 II JOINT LINKAGE DISEQUILIBRIUM AND LINKAGE MAP- PING FOR COMPLEX PEDIGREES . . . . . . . . . . . . . . . 5 2...

  12. GenePRIMP: A GENE PRediction IMprovement Pipeline for Prokaryotic genomes

    E-Print Network [OSTI]

    Pati, Amrita

    2012-01-01T23:59:59.000Z

    PRediction IMprovement Pipeline for Amrita Pati 1 , NataliaGene Prediction IMprovement Pipeline, http://geneprimp.jgi-based post-processing pipeline that identifies erroneously

  13. The Universe Viewed in Gamma-Rays 1 Properties of Gamma-ray Bursts Localized by

    E-Print Network [OSTI]

    Enomoto, Ryoji

    The Universe Viewed in Gamma-Rays 1 Properties of Gamma-ray Bursts Localized by the HETE-2 and localize Gamma-ray bursts (GRBs) in wide field of view. HETE-2 have been localized about 20 GRBs per year hours after the burst. 1. The High Energy Transient Explorer 2 Gamma-ray burst (GRB) is the most

  14. Modeling 3D animals from a side-view sketch Even Entema,b

    E-Print Network [OSTI]

    Barthe, Loïc

    Modeling 3D animals from a side-view sketch Even Entema,b , Loic Barthea , Marie-Paule Canib. This paper tackles the problem of creating 3D models of animals from a single, side-view sketch. We use be an important step for generat- ing more lively virtual worlds. Animals are also among the models

  15. Reconstructions in limited-view thermoacoustic tomography Yuan Xu and Lihong V. Wanga)

    E-Print Network [OSTI]

    Ambartsoumian, Gaik

    Reconstructions in limited-view thermoacoustic tomography Yuan Xu and Lihong V. Wanga) Optical for publication 9 December 2003; published 11 March 2004 The limited-view problem is studied for thermoacoustic of Physicists in Medicine. DOI: 10.1118/1.1644531 Key words: thermoacoustic tomography, photoacoustic tomography

  16. NEED AND METHODS OF GENE CONSERVATION IN ANIMAL BREEDING (*)

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    new useful variation. The available methods of gene conservation were discussed, the main attention being paid to the maintenance of small nuclei of most breeds and strains, to the establishment of gene of genes is deter- mined by several groups of factors, e. g. theoccurrence of actual gene losses

  17. Gene for ataxia-telangiectasia complementation group D (ATDC)

    DOE Patents [OSTI]

    Murnane, J.P.; Painter, R.B.; Kapp, L.N.; Yu, L.C.

    1995-03-07T23:59:59.000Z

    Disclosed herein is a new gene, an AT gene for complementation group D, the ATDC gene and fragments thereof. Nucleic acid probes for the gene are provided as well as proteins encoded by the gene, cDNA therefrom, preferably a 3 kilobase (kb) cDNA, and recombinant nucleic acid molecules for expression of the proteins. Further disclosed are methods to detect mutations in the gene, preferably methods employing the polymerase chain reaction (PCR). Also disclosed are methods to detect AT genes from other AT complementation groups. 30 figs.

  18. Increasing Cancer-Specific Gene Expression by Targeting Overexpressed 51 Integrin and Upregulated Transcriptional

    E-Print Network [OSTI]

    Kokkoli, Efie

    Engineering and Materials Science, University of Minnesota, 421 Washington Ave SE, Minneapolis, Minnesota the control of an NF-B responsive element (pNF-B- Luc) encapsulated in a PR_b peptide functionalized stealth was seen when transfected with PR_b functionalized stealth liposomes encapsulating the condensed p

  19. Gene Lopez Edgar Howard Gene Lopez and Edgar Howard both taught Mathematics at San Diego State for over 30 years.

    E-Print Network [OSTI]

    Gallo, Linda C.

    Gene Lopez Edgar Howard Gene Lopez and Edgar Howard both taught Mathematics at San Diego State and Computer Sciences is establishing The Gene Lopez and Edgar Howard Memorial Fund Donations to this fund that this is especially appropriate as a memorial to Gene and Edgar since each of them contributed significantly

  20. Low Rates of Expression Profile Divergence in Highly Expressed Genes and Tissue-Specific Genes During Mammalian Evolution

    E-Print Network [OSTI]

    Zhang, Jianzhi

    Low Rates of Expression Profile Divergence in Highly Expressed Genes and Tissue-Specific Genes specificity influence the divergence of expression profiles between orthologous genes. Here we address expression profile change during evolution is negatively correlated with the level of gene expression

  1. GeneTracer: Gene Sequence Analysis of Disease Mutations VAST 2010 Mini Challenge 3 Award: Excellent Process Explanation

    E-Print Network [OSTI]

    Stasko, John T.

    GeneTracer: Gene Sequence Analysis of Disease Mutations VAST 2010 Mini Challenge 3 Award: ExcellentTracer, developed for the VAST 2010 genetic sequence mini challenge, visualizes gene sequences of cur- rent outbreaks and native sequences along with disease character- istics. We successfully used Gene

  2. Gene Expression Patterns and Gene Copy Number Changes in Dermatofibrosarcoma Protuberans

    E-Print Network [OSTI]

    Botstein, David

    of Anatomic Pathology,¶ Cleveland Clinic Foundation, Cleveland, Ohio Dermatofibrosarcoma protuberans (DFSP:22), which fuses the COL1A1 and PDGF genes. We determined the characteristic gene expression profile of DFSP and characterized DNA copy number changes in DFSP by array-based comparative genomic hybridization (ar- ray CGH

  3. DeepView: A collaborative framework for distributed microscopy

    SciTech Connect (OSTI)

    Parvin, B.; Taylor, J.; Cong, G.

    1998-08-10T23:59:59.000Z

    This paper outlines the motivation, requirements, and architecture of a collaborative framework for distributed virtual microscopy. In this context, the requirements are specified in terms of (1) functionality, (2) scalability, (3) interactivity, and (4) safety and security. Functionality refers to what and how an instrument does something. Scalability refers to the number of instruments, vendor-specific desktop workstations, analysis programs, and collaborators that can be accessed. Interactivity refers to how well the system can be steered either for static or dynamic experiments. Safety and security refers to safe operation of an instrument coupled with user authentication, privacy, and integrity of data communication. To meet these requirements, we introduce three types of services in the architecture: Instrument Services (IS), Exchange Services (ES), and Computational Services (CS). These services may reside on any host in the distributed system. The IS provide an abstraction for manipulating different types of microscopes; the ES provide common services that are required between different resources; and the CS provide analytical capabilities for data analysis and simulation. These services are brought together through CORBA and its enabling services, e.g., Event Services, Time Services, Naming Services, and Security Services. Two unique applications have been introduced into the CS for analyzing scientific images either for instrument control or recovery of a model for objects of interest. These include: in-situ electron microscopy and recovery of 3D shape from holographic microscopy. The first application provides a near real-time processing of the video-stream for on-line quantitative analysis and the use of that information for closed-loop servo control. The second application reconstructs a 3D representation of an inclusion (a crystal structure in a matrix) from multiple views through holographic electron microscopy. These application require steering external stimuli or computational parameters for a particular result. In a sense, ''computational instruments'' (symmetric multiprocessors) interact closely with data generated from ''experimental instruments'' (unique microscopes) to conduct new experiments and bring new functionalities to these instruments. Both of these features exploit high-performance computing and low-latency networks to bring novel functionalities to unique scientific imaging instruments.

  4. Id-1 gene and gene products as therapeutic targets for treatment of breast cancer and other types of carcinoma

    SciTech Connect (OSTI)

    Desprez, Pierre-Yves; Campisi, Judith

    2014-08-19T23:59:59.000Z

    A method for treatment of breast cancer and other types of cancer. The method comprises targeting and modulating Id-1 gene expression, if any, for the Id-1 gene, or gene products in breast or other epithelial cancers in a patient by delivering products that modulate Id-1 gene expression. When expressed, Id-1 gene is a prognostic indicator that cancer cells are invasive and metastatic.

  5. Pathogenicity island mobility and gene content.

    SciTech Connect (OSTI)

    Williams, Kelly Porter

    2013-10-01T23:59:59.000Z

    Key goals towards national biosecurity include methods for analyzing pathogens, predicting their emergence, and developing countermeasures. These goals are served by studying bacterial genes that promote pathogenicity and the pathogenicity islands that mobilize them. Cyberinfrastructure promoting an island database advances this field and enables deeper bioinformatic analysis that may identify novel pathogenicity genes. New automated methods and rich visualizations were developed for identifying pathogenicity islands, based on the principle that islands occur sporadically among closely related strains. The chromosomally-ordered pan-genome organizes all genes from a clade of strains; gaps in this visualization indicate islands, and decorations of the gene matrix facilitate exploration of island gene functions. A %E2%80%9Clearned phyloblocks%E2%80%9D method was developed for automated island identification, that trains on the phylogenetic patterns of islands identified by other methods. Learned phyloblocks better defined termini of previously identified islands in multidrug-resistant Klebsiella pneumoniae ATCC BAA-2146, and found its only antibiotic resistance island.

  6. Optic for an endoscope/borescope having high resolution and narrow field of view

    DOE Patents [OSTI]

    Stone, Gary F.; Trebes, James E.

    2003-10-28T23:59:59.000Z

    An optic having optimized high spatial resolution, minimal nonlinear magnification distortion while at the same time having a limited chromatic focal shift or chromatic aberrations. The optic located at the distal end of an endoscopic inspection tool permits a high resolution, narrow field of view image for medical diagnostic applications, compared to conventional optics for endoscopic instruments which provide a wide field of view, low resolution image. The image coverage is over a narrow (<20 degrees) field of view with very low optical distortion (<5% pin cushion or barrel distortion. The optic is also optimized for best color correction as well as to aid medical diagnostics.

  7. Universal light-switchable gene promoter system

    DOE Patents [OSTI]

    Quail, Peter H.; Huq, Enamul; Tepperman, James; Sato, Sae

    2005-02-22T23:59:59.000Z

    An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.

  8. Remote Participation at EFDA­JET Task Force Work, Experience and Priorities from a User’s Point of View

    E-Print Network [OSTI]

    Remote Participation at EFDA­JET Task Force Work, Experience and Priorities from a User’s Point of View

  9. Correction of human. beta. sup S -globin gene by gene targeting

    SciTech Connect (OSTI)

    Shesely, E.G.; Hyungsuk Kim; Shehee, W.R.; Smithies, O. (Univ. of North Carolina, Chapel Hill (United States)); Papayannopoulou, T. (Univ. of Washington, Seattle (United States)); Popovich, B.W. (Children's Hospital, San Diego, CA (United States))

    1991-05-15T23:59:59.000Z

    As a step toward using gene targeting for gene therapy, the authors have corrected a human {beta}{sup S}-globin gene to the normal {beta}{sup A} allele by homologous recombination in the mouse-human hybrid cell line BSM. BSM is derived from a mouse erythroleukemia cell line and carries a single human chromosome 11 with the {beta}{sup S}-globin allele. A {beta}{sup A}-globin targeting construct containing a unique oligomer and a neomycin-resistance gene was electroporated into the BSM cells, which were then placed under G418 selection. Then 126 resulting pools containing a total {approx}29,000 G418-resistant clones were screened by PCR for the presence of a targeted recombinant: 3 positive pools were identified. A targeted clone was isolated by replating one of the positive pools into smaller pools and rescreening by PCR, followed by dilution cloning. Southern blot analysis demonstrated that the isolated clone had been targeted as planned. The correction of the {beta}{sup S} allele to {beta}{sup A} was confirmed both by allele-specific PCR and by allele-specific antibodies. Expression studies comparing the uninduced and induced RNA levels in unmodified BSM cells and in the targeted clone showed no significant alteration in the ability of the targeted clone to undergo induction, despite the potentially disrupting presence of a transcriptionally active neomycin gene 5{prime} to the human {beta}{sup A}-globin gene. Thus gene targeting can correct a {beta}{sup S} allele to {beta}{sup A}, and the use of a selectable helper gene need not significantly interfere with the induction of the corrected gene.

  10. Moving Toward a Vision of Family Independence: Local Managers' Views of Michigan's Welfare Reforms

    E-Print Network [OSTI]

    Shyy, Wei

    Reforms Kristin S. Seefeldt Jodi Sandfort Sandra K. Danziger February, 1998 This project is supported? .................................................................................39 SECTION IV: MANAGERS' VIEW OF WELFARE REFORM AND IMPLEMENTATION ISSUES......................................................................44 Support for Reform Initiatives

  11. THESIS FOR THE DEGREE OF LICENTIATE OF ENGINEERING View Frustum Culling and Animated Ray Tracing

    E-Print Network [OSTI]

    Assarsson, Ulf

    THESIS FOR THE DEGREE OF LICENTIATE OF ENGINEERING View Frustum Culling and Animated Ray Tracing Thesis for the degree of Licentiate of Engineering, a Swedish degree between M.Sc. and Ph.D. Abstract

  12. DOE Zero Energy Ready Home Case Study 2014: Clifton View Homes...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    founder of Clifton View Homes and Zero-Energy Plans, LLC, strives to go beyond net zero energy construction so that every home he designs and builds can produce enough...

  13. Direct imaging with highly diluted apertures. I. Field of view limitations

    E-Print Network [OSTI]

    O. Lardiere; F. Martinache; F. Patru

    2006-12-04T23:59:59.000Z

    Future optical interferometric instrumentation mainly relies on the availability of an efficient cophasing system: once available, what has so far postponed the relevance of direct imaging with an interferometer will vanish. This paper focuses on the actual limits of snapshot imaging, inherent to the use of a sparse aperture: the number of telescopes and the geometry of the array impose the maximum extent of the field of view and the complexity of the sources. A second limitation may arise from the beam combination scheme. Comparing already available solutions, we show that the so called hypertelescope mode (or densified pupil) is ideal. By adjusting the direct imaging field of view to the useful field of view offered by the array, the hypertelescope makes an optimal use of the collected photons. It optimizes signal to noise ratio, drastically improves the luminosity of images and makes the interferometer compatible with coronagraphy, without inducing any loss of useful field of view.

  14. California’s Energy Future: The View to 2050 - Summary Report

    E-Print Network [OSTI]

    Yang, Christopher

    2011-01-01T23:59:59.000Z

    carbon capture and storage, especially as a technology thatCarbon capture and sequestration CCST California Council on Science and Technologytechnology California’s Energy Future - The View to 2050 becomes available. ? ? Fossil fuel with carbon capture

  15. Improving Usability of Interactive Graphics Specification and Implementation with Picking Views

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Improving Usability of Interactive Graphics Specification and Implementation with Picking Views.conversy@enac.fr Abstract-- Specifying and programming graphical interactions are difficult tasks, notably because designers architecture improves the usability of the specification and the implementation of graphical interaction

  16. Appendix A Organizational Charts UT System Administration Table of Organization can be viewed at

    E-Print Network [OSTI]

    Cui, Yan

    85 Appendix A ­ Organizational Charts UT System Administration Table of Organization can be viewed can be found at www.uthsc.edu/administration/. An Organizational Chart for UTHSC is shown below. #12

  17. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    E-Print Network [OSTI]

    Hoen, Ben

    2012-01-01T23:59:59.000Z

    impacts of wind energy facilities on the sales prices ofprices were affected by views of and proximity to wind energyprices, and locations in electronic form from local assessors; and (3) the representativeness of the types of wind energy

  18. CONTENT-BASED 3D OBJECT RETRIEVAL USING 2D VIEWS Thibault Napoleon

    E-Print Network [OSTI]

    Sahbi, Hichem

    CONTENT-BASED 3D OBJECT RETRIEVAL USING 2D VIEWS Thibault Napol´eon TELECOM ParisTech, CNRS UMR 5141 46 rue Barrault 75013 Paris, France napoleon@telecom-paristech.fr Hichem Sahbi CNRS UMR 5141

  19. Ecology in Africa: a view from the past for informing the future

    E-Print Network [OSTI]

    Martins, Dino J.

    2013-01-01T23:59:59.000Z

    reviews ISSN 1948?6596  Ecology in Africa: a view from the the future  African Ecology – Benchmarks and Historical s  role  in  shaping  the  ecology  of  Africa…”.   These 

  20. UFilter: A Lightweight XML View Update Checker Ling Wang, Elke A. Rundensteiner and Murali Mani

    E-Print Network [OSTI]

    U­Filter: A Lightweight XML View Update Checker Ling Wang, Elke A. Rundensteiner and Murali Mani 1997 year Simon & Schuster Inc. A02 Prentice­Hall Inc. B01 McGraw­Hill

  1. Automated Deduction: A Technological Point of View Peter Baumgartner, Ingo Dahn, Jrgen Dix, Ulrich Furbach,

    E-Print Network [OSTI]

    Baumgartner, Peter

    1 Automated Deduction: A Technological Point of View Peter Baumgartner, Ingo Dahn, Jürgen Dix called ILF, developed by I. Dahn within the German focus program on Deduction, was able to translate

  2. Cellulosic Biofuels: Expert Views on Prospects for Advancement and Jeffrey Keisler

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    Cellulosic Biofuels: Expert Views on Prospects for Advancement Erin Baker and Jeffrey Keisler funding and the likelihood of achieving advances in cellulosic biofuel technologies. While in collecting more information on this technology. Keywords: Biofuels; Technology R&D; Uncertainty

  3. Lifecycle Environments: A Retrospective View of the Contributions of Leon J.

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    Lifecycle Environments: A Retrospective View of the Contributions of Leon J. Osterweil Lori A@cs.umass.edu Abstract Throughout his career, Leon Osterweil has made significant contribu- tions that have impacted

  4. U.S. Science Journalists' Views and Uses of Online Reader Comments: A Qualitative Study 

    E-Print Network [OSTI]

    Schaefer, Mary Elizabeth

    2014-09-02T23:59:59.000Z

    publications also provide insight into how some journalists view comments. In an article on the Nieman Reports website, an NPR ombudsman recounts some of her experiences with comments, which have consisted largely of personal attacks, and addresses issues...

  5. Computing point-of-view : modeling and simulating judgments of taste

    E-Print Network [OSTI]

    Liu, Xinyu Hugo, 1979-

    2006-01-01T23:59:59.000Z

    People have rich points-of-view that afford them the ability to judge the aesthetics of people, things, and everyday happenstance; yet viewpoint has an ineffable quality that is hard to articulate in words, let alone capture ...

  6. Negotiating authenticity: a negotiated order study of Valley View Summer Camp

    E-Print Network [OSTI]

    Free, Rebecca J

    1999-01-01T23:59:59.000Z

    the negotiated order of Valley View's summer camp from June 1998 until August 1998. Economic needs, organizational growth, the increasing violent behavior of children, and the mission and history of the camp were aspects of the structural context impacting...

  7. TerraView Plugins HowTo Revision date 12/12/2006

    E-Print Network [OSTI]

    View plugin structure uses a plugin layer library called LibSLP ( see SPL web site5 for reference files (.pro) by using the QT tool called "qmake". Check QT web site for reference. BASE.PRO BASE

  8. GPU-based Scalable Volumetric Reconstruction for Multi-view Stereo

    SciTech Connect (OSTI)

    Kim, H; Duchaineau, M; Max, N

    2011-09-21T23:59:59.000Z

    We present a new scalable volumetric reconstruction algorithm for multi-view stereo using a graphics processing unit (GPU). It is an effectively parallelized GPU algorithm that simultaneously uses a large number of GPU threads, each of which performs voxel carving, in order to integrate depth maps with images from multiple views. Each depth map, triangulated from pair-wise semi-dense correspondences, represents a view-dependent surface of the scene. This algorithm also provides scalability for large-scale scene reconstruction in a high resolution voxel grid by utilizing streaming and parallel computation. The output is a photo-realistic 3D scene model in a volumetric or point-based representation. We demonstrate the effectiveness and the speed of our algorithm with a synthetic scene and real urban/outdoor scenes. Our method can also be integrated with existing multi-view stereo algorithms such as PMVS2 to fill holes or gaps in textureless regions.

  9. Intrinsic modulation of ENSO predictability viewed through a local Lyapunov lens

    E-Print Network [OSTI]

    Wittenberg, Andrew

    Intrinsic modulation of ENSO predictability viewed through a local Lyapunov lens Christina magnitude, frequency, and physical evolution. Local Lyapunov exponents (LLEs) estimated from the monthly Predictability Á Local Lyapunov exponents 1 Introduction The limits of predictability of the state

  10. 176 nature nanotechnology | VOL 5 | MARCH 2010 | www.nature.com/naturenanotechnology news & views

    E-Print Network [OSTI]

    Buratto, Steve

    176 nature nanotechnology | VOL 5 | MARCH 2010 | www.nature.com/naturenanotechnology news & views P humidity. Writing in Nature Nanotechnology, Saeed Moghaddam, Mark Shannon and colleagues1 at the University

  11. Regulation of methane genes and genome expression

    SciTech Connect (OSTI)

    John N. Reeve

    2009-09-09T23:59:59.000Z

    At the start of this project, it was known that methanogens were Archaeabacteria (now Archaea) and were therefore predicted to have gene expression and regulatory systems different from Bacteria, but few of the molecular biology details were established. The goals were then to establish the structures and organizations of genes in methanogens, and to develop the genetic technologies needed to investigate and dissect methanogen gene expression and regulation in vivo. By cloning and sequencing, we established the gene and operon structures of all of the “methane” genes that encode the enzymes that catalyze methane biosynthesis from carbon dioxide and hydrogen. This work identified unique sequences in the methane gene that we designated mcrA, that encodes the largest subunit of methyl-coenzyme M reductase, that could be used to identify methanogen DNA and establish methanogen phylogenetic relationships. McrA sequences are now the accepted standard and used extensively as hybridization probes to identify and quantify methanogens in environmental research. With the methane genes in hand, we used northern blot and then later whole-genome microarray hybridization analyses to establish how growth phase and substrate availability regulated methane gene expression in Methanobacterium thermautotrophicus ?H (now Methanothermobacter thermautotrophicus). Isoenzymes or pairs of functionally equivalent enzymes catalyze several steps in the hydrogen-dependent reduction of carbon dioxide to methane. We established that hydrogen availability determine which of these pairs of methane genes is expressed and therefore which of the alternative enzymes is employed to catalyze methane biosynthesis under different environmental conditions. As were unable to establish a reliable genetic system for M. thermautotrophicus, we developed in vitro transcription as an alternative system to investigate methanogen gene expression and regulation. This led to the discovery that an archaeal protein, designated TFE, that had sequences in common with the eukaryotic general transcription factor TFIIE, stimulated archaeal transcription initiation and that the archaeal TATA-box binding protein (TBP) remained attached to the promoter region whereas the transcription factor TFB dissociated from the template DNA following initiation. DNA sequences that directed the localized assembly of archaeal histones into archaeal nucleosomes were identified, and we established that transcription by an archaeal RNA polymerase was slowed but not blocked by archaeal nucleosomes. We developed a new protocol to purify archaeal RNA polymerases and with this enzyme and additional improvements to the in vitro transcription system, we established the template requirements for archaeal transcription termination, investigated the activities of proteins predicted to be methane gene regulators, and established how TrpY, a novel archaeal regulator of expression of the tryptophan biosynthetic operon functions in M. thermautotrophicus. This also resulted in the discovery that almost all M. thermautotrophicus mutants isolated as spontaneously resistant to 5-methyl tryptophan (5MTR) had mutations in trpY and were therefore 5MTR through de-repressed trp operon expression. This established a very simple, practical procedure to determine and quantify the DNA sequence changes that result from exposure of this Archaeon to any experimental mutagenesis protocol. Following the discovery that the Thermococcus kodakaraensis was amenable to genetic manipulation, we established this technology at OSU and subsequently added plasmid expression, a reporter system and additional genetic selections to the T. kodakaraensis genetic toolbox. We established that transcription and translation are coupled in this Archaeon, and by combining in vitro transcription and in vivo genetics, we documented that both TFB1 and TFB2 support transcription initiation in T. kodakaraensis. We quantified the roles of ribosome binding sequences and alternative initiation codons in translation initiation, established that polarity e

  12. What phylogeny and gene genealogy analyses reveal about homoplasy in citrus microsatellite alleles

    E-Print Network [OSTI]

    Barkley, Noelle A.; Krueger, Robert R.; Federici, Claire T.; Roose, Mikeal L.

    2009-01-01T23:59:59.000Z

    What phylogeny and gene genealogy analyses reveal aboutAdditionally, gene genealogies (networks) were constructedtaxa. Networks/gene genealogies TCS version 1.13 (Clement et

  13. Gene Expression in the Stallion Testes

    E-Print Network [OSTI]

    Laughlin, Andy M.

    2011-08-08T23:59:59.000Z

    genes were differentially expressed (122 in fertile tissue, 111 in subfertile tissue). Of these, phosphodiesterase 3B (PDE3B), steroidogenic acute regulatory (StAR) protein, and outer dense fiber of sperm tails 2 (ODF2) mRNAs, were localized...

  14. Gene duplications, robustness and evolutionary innovations

    E-Print Network [OSTI]

    Wagner, Andreas

    , flowering plant evolution and heart development, which encompass some of the most striking innovationsGene duplications, robustness and evolutionary innovations Andreas Wagner1,2,3 Summary Mutational with evolutionary innovation is thus a special case of a general mechanism linking innovation to robustness

  15. Gene encoding acetyl-coenzyme A carboxylase

    DOE Patents [OSTI]

    Roessler, Paul G. (Golden, CO); Ohlrogge, John B. (Okemos, MI)

    1996-01-01T23:59:59.000Z

    A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.

  16. Gene encoding acetyl-coenzyme A carboxylase

    DOE Patents [OSTI]

    Roessler, P.G.; Ohlrogge, J.B.

    1996-09-24T23:59:59.000Z

    A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives are disclosed which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides. 5 figs.

  17. SHORT REVIEW Ecological genomics: understanding gene and

    E-Print Network [OSTI]

    Kaufman, Glennis A.

    SHORT REVIEW Ecological genomics: understanding gene and genome function in the natural environment MC Ungerer, LC Johnson and MA Herman Division of Biology, Ecological Genomics Institute, Kansas State University, Manhattan, KS, USA The field of ecological genomics seeks to understand the genetic mechanisms

  18. ORIGINAL ARTICLE Viral photosynthetic reaction center genes

    E-Print Network [OSTI]

    Mandel-Gutfreund, Yael

    , Technion--Israel Institute of Technology, Haifa, Israel; 3 Inter-Departmental Program for Biotechnology. The discovery of genes (psbA, psbD) that encode key photosystem II proteins (D1, D2) in the genomes of phages the infection process. Here, using metagenomic data in natural ocean samples, we show that about 60% of the psb

  19. COMPUTATIONAL COMPARATIVE GENOMICS: GENES, REGULATION, EVOLUTION

    E-Print Network [OSTI]

    Kellis, Manolis

    COMPUTATIONAL COMPARATIVE GENOMICS: GENES, REGULATION, EVOLUTION by Manolis (Kellis) Kamvysselis B.S. Electrical Engineering and Computer Science; M. Eng. Computer Science and Engineering Massachusetts Institute at the Massachusetts Institute of Technology June 2003 © 2003 Massachusetts Institute of Technology All rights reserved

  20. BIOINFORMATICS Comparative Evaluation of Reverse Engineering Gene

    E-Print Network [OSTI]

    Babu, M. Madan

    BIOINFORMATICS Comparative Evaluation of Reverse Engineering Gene Regulatory Networks of Statistics, University of Dortmund, Germany ABSTRACT Motivation: An important problem in systems biology into account. This suggests that the higher computational costs of infe- rence with BNs over GGMs and RNs

  1. Cross-regulation and interaction between eukaryotic gene regulatory processes

    E-Print Network [OSTI]

    Spies, Noah (Noah Walter Benjamin)

    2012-01-01T23:59:59.000Z

    Regulation of genes is fundamental to all living processes and can be exerted at many sequential steps. We studied several eukaryotic gene regulatory mechanisms with an emphasis on understanding the interplay between ...

  2. Text Mining in Cancer Gene and Pathway Prioritization

    E-Print Network [OSTI]

    Riedlinger, Gregory

    Prioritization of cancer implicated genes has received growing attention as an effective way to reduce wet lab cost by computational analysis that ranks candidate genes according to the likelihood that experimental ...

  3. Transcriptional regulation constrains the organization of genes on eukaryotic chromosomes

    E-Print Network [OSTI]

    Babu, M. Madan

    Transcriptional regulation constrains the organization of genes on eukaryotic chromosomes Sarath. Although it is known that eukaryotic transcriptional regulation is complex and requires an intricate-order organization of genes across and within chromosomes that is constrained by transcriptional regulation

  4. asthma candidate genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    F.C. 38 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  5. autism candidate gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    F.C. 46 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  6. adhd candidate gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    F.C. 34 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  7. axon guidance gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Index 1 The axon guidance receptor gene ROBO1 is a candidate gene for developmental dyslexia CiteSeer Summary: Dyslexia, or specific reading disability, is the most common...

  8. asthma susceptibility gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in the IL13 gene and atopy in all three populations (and with asthma 2005-01-01 6 The Dyslexia Susceptibility Genes and Neurodevelopment. Open Access Theses and Dissertations...

  9. amoa gene analysis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    applied to determine the core cell cycle genes in the recently Gent, Universiteit 264 Graeber et al. 2013 -Roles of Lepidium Seed Dormancy Genes 1 Spatio-temporal Seed Development...

  10. analysis reveals genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    applied to determine the core cell cycle genes in the recently Gent, Universiteit 466 Graeber et al. 2013 -Roles of Lepidium Seed Dormancy Genes 1 Spatio-temporal Seed Development...

  11. analysis identifies genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    applied to determine the core cell cycle genes in the recently Gent, Universiteit 490 Graeber et al. 2013 -Roles of Lepidium Seed Dormancy Genes 1 Spatio-temporal Seed Development...

  12. Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering

    E-Print Network [OSTI]

    Bolliet, Catherine

    2007-01-01T23:59:59.000Z

    Recent advances in tissue engineering, combining an extracellular matrix (ECM)-like vehicle with therapeutic molecules, cells and/or genes has yielded promising results for brain injury repair. The purpose of this thesis ...

  13. Gene Expression Analyses and Association Studies of Wood Development Genes in Loblolly Pine (Pinus taeda L.)

    E-Print Network [OSTI]

    Palle, Sreenath Reddy

    2011-10-21T23:59:59.000Z

    ............................................................ 58 7 Model of the association between the AIP and PRT genes ........................ 64 8 Pathways demonstrating the indirect role of CGS and the direct role of CAD in lignin biosynthesis... complex and therefore the molecular mechanisms governing the differentiation of wood tissues are complicated. Several researchers have identified a number of genes involved in the biosynthesis of polysaccharides, lignins and cell wall proteins in forest...

  14. Gene co-expression network analysis in Rhodobacter capsulatus...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    annotation. We identified R. capsulatus modules enriched with genes for ribosomal proteins, porphyrin and bacteriochlorophyll anabolism, and biosynthesis of secondary...

  15. Message from ISCB Getting Started in Gene Orthology and Functional

    E-Print Network [OSTI]

    Gerstein, Mark

    . Each genome consists of a unique gene inventory, which determines the specific phenotype species by analyzing compositions of gene inventories therefore opens the door to the rich branch can only be identified when the whole gene inventories from all the involved species are examined

  16. Generator based Modeling CP Game for Gene Regulatory Network

    E-Print Network [OSTI]

    Lescanne, Pierre

    introduces a framework based on game theory that models gene regulation activities. Strategic games which are the basic model in game theory was successfully applied to gene regulation networks and molecular networks be established. One of the main issue when a gene regulation system is analyzed through a model is to decompose

  17. 2005 Nature Publishing Group Photosynthesis genes in marine viruses yield

    E-Print Network [OSTI]

    Church, George M.

    © 2005 Nature Publishing Group Photosynthesis genes in marine viruses yield proteins during host­6 probably influences the genetic and functional diversity of both. For example, photosynthesis genes period. We also show that the expression of host photosynthesis genes declines over the course

  18. Efficient and Robust Algorithms for Statistical Inference in Gene Regulatory Networks

    E-Print Network [OSTI]

    Noor, Amina

    2013-12-10T23:59:59.000Z

    Inferring gene regulatory networks (GRNs) is of profound importance in the ?eld of computational biology and bioinformatics. Understanding the gene-gene and gene- transcription factor (TF) interactions has the potential of providing an insight...

  19. Identification of novel lung genes in bronchial epithelium by serial analysis of gene Kim M. Lonergan*1

    E-Print Network [OSTI]

    Ng, Raymond T.

    1 Identification of novel lung genes in bronchial epithelium by serial analysis of gene expression of human bronchial epithelium should provide a basis for studying lung diseases including cancer. We have deduced global gene expression profiles of bronchial epithelium and lung parenchyma, based upon a vast

  20. DNA methylation map of mouse and human brain identifies target genes in Alzheimer's disease

    E-Print Network [OSTI]

    2013-01-01T23:59:59.000Z

    al. Figure 3 Epigenetic deregulation of target genes in the3027 Figure 2 Epigenetic deregulation of target genes in

  1. 614 | VOL.4 NO.8 | AUGUST 2007 | NATURE METHODS NEWS AND VIEWS

    E-Print Network [OSTI]

    Cai, Long

    . Because the data are sequence reads, ChIP-seq offers a rapid analysis pipeline (as long as a high-site sequences,which may directly support any observed changes in protein binding and gene regulation

  2. nature methods | VOL.9 NO.10 | OCTOBER 2012 | 961 news and views

    E-Print Network [OSTI]

    Zhou, Xianghong Jasmine

    and computational pipeline for high-resolution 4C-seq1, and the second reports a new strategy for simultaneously into close proximity by chromatin interac- tions, transforming our understanding of gene regulation

  3. Remote Viewing End Effectors for Light Duty Utility Arm Robot (U)

    SciTech Connect (OSTI)

    Heckendorn, F.M. [Westinghouse Savannah River Company, AIKEN, SC (United States); Robinson, C.W.; Haynes, H.B.; Anderosn, E.K.; Pardini, A.F. [Westinghouse Hanford Co., Richland, WA (United States)

    1996-11-04T23:59:59.000Z

    The Robotics Development Groups at the Savannah River Site (SRS) and at the Hanford site have developed remote video and photography systems for deployment in underground radioactive-waste storage tanks at the Department of Energy (DOE) sites as a part of the Office of Science and Technology (OST) program within DOE. Viewing and documenting the tank interiors and their associated annular spaces is an extremely valuable tool in characterizing their condition and contents and in controlling their remediation. Several specialized video/photography systems and robotic End Effectors have been fabricated that provide remote viewing and lighting. All are remotely deployable into and out of the tank, with all viewing functions remotely operated. Positioning all control components away from the facility prevents the potential for personnel exposure to radiation and contamination. Only the remote video systems are discussed in this paper.

  4. "A force illegible": A study of Emily Dickinson's view of God

    E-Print Network [OSTI]

    Thompson, Margaret Henderson

    1970-01-01T23:59:59.000Z

    "A FORCE ILLEGIBLE": A STUDY OF EMILY DICKINSON'S VIEW OF GOD A Thesis by MARGARET HENDERSON THOMPSON Submitted to the Graduate College of Texas AErM University in partial fulfillment of the requirement for the degree of MASTER OF ARTS... December 1970 Major Subject: English "A FORCE ILLEGIBI v": A STUDY OF EMILY DICKINSON'S VIEW OF GOD A Thesis by MARGARET HENDERSON THOMPSON Approved as to style and content by: ~Ai'-&~ ei . ~ --~, (y~. g" ~z-7& j a1rman 0 omml. ttee Hea o...

  5. Television Viewing, Walking Speed and Grip Strength in a Prospective Cohort Study

    E-Print Network [OSTI]

    Keevil, Victoria L.; Wijndaele, Katrien; Luben, Robert; Sayer, Avan A.; Wareham, Nicholas J.; Khaw, Kay-Tee

    2014-07-18T23:59:59.000Z

    , strong and consistent associations between grip strength and TV viewing time were not observed. Isometric muscle strength encapsulates only one aspect of muscle func- tion, and low performance may be present without low strength or vice versa, a concept... for age, physical activity, anthropome- try, wealth, comorbidity, smoking, and alcohol intake and combined if no sex–TV viewing time interactions were identified. Results: Men and women who watched the least TV at the 2HC or 3HC walked at a faster usual...

  6. New cationic lipids for gene transfer with high efficiency and low toxicity: T-shape cholesterol ester derivatives

    E-Print Network [OSTI]

    Park, Jong-Sang

    ester derivatives Yan Lee,a Heebeom Koo,a Yong-beom Lim,b Youngeun Lee,a Heejung Moa and Jong Sang Parka of the relationship between the molecular structures and the transfection efficiency. Lee et al. synthesized many to overcome the other obstacle like cytotoxicity of cationic lipids. Introduction of biodegradable bonds

  7. The Role of Multiple Transcription Factors In Archaeal Gene Expression

    SciTech Connect (OSTI)

    Charles J. Daniels

    2008-09-23T23:59:59.000Z

    Since the inception of this research program, the project has focused on two central questions: What is the relationship between the 'eukaryal-like' transcription machinery of archaeal cells and its counterparts in eukaryal cells? And, how does the archaeal cell control gene expression using its mosaic of eukaryal core transcription machinery and its bacterial-like transcription regulatory proteins? During the grant period we have addressed these questions using a variety of in vivo approaches and have sought to specifically define the roles of the multiple TATA binding protein (TBP) and TFIIB-like (TFB) proteins in controlling gene expression in Haloferax volcanii. H. volcanii was initially chosen as a model for the Archaea based on the availability of suitable genetic tools; however, later studies showed that all haloarchaea possessed multiple tbp and tfb genes, which led to the proposal that multiple TBP and TFB proteins may function in a manner similar to alternative sigma factors in bacterial cells. In vivo transcription and promoter analysis established a clear relationship between the promoter requirements of haloarchaeal genes and those of the eukaryal RNA polymerase II promoter. Studies on heat shock gene promoters, and the demonstration that specific tfb genes were induced by heat shock, provided the first indication that TFB proteins may direct expression of specific gene families. The construction of strains lacking tbp or tfb genes, coupled with the finding that many of these genes are differentially expressed under varying growth conditions, provided further support for this model. Genetic tools were also developed that led to the construction of insertion and deletion mutants, and a novel gene expression scheme was designed that allowed the controlled expression of these genes in vivo. More recent studies have used a whole genome array to examine the expression of these genes and we have established a linkage between the expression of specific tfb genes and the regulation of nitrogen metabolism and other global cellular responses.

  8. New Gene Mutations Tied to Schizophrenia -Health News and Views -Health.com http://news.health.com/2012/10/08/new-gene-mutations-tied-to-schizophrenia/[11/12/2012 12:16:20 PM

    E-Print Network [OSTI]

    Diabetes1 Eating Family Fibromyalgia Fitness GERD goodhealth Gout Health News Heart Disease Heartburn HIV

  9. Working Towards Reform in Mathematics Education: Parents', Teachers', and Students' Views of "Different"1

    E-Print Network [OSTI]

    Civil, Marta

    Working Towards Reform in Mathematics Education: Parents', Teachers', and Students' Views presented at the ACCLAIM Research Symposium 3, Mathematics Education: Reform and Resistance;2 This essay is a reflection on several aspects related to my encounters with the concept of reform

  10. The Universe Viewed in Gamma-Rays 1 Concept of new gamma ray detector

    E-Print Network [OSTI]

    Enomoto, Ryoji

    a sensitivity of a detector at TeV gamma ray range. This method was used for a non-imaging detector as XrayThe Universe Viewed in Gamma-Rays 1 Concept of new gamma ray detector Satoko Osone Institute Abstract We present a concept of a new gamma ray detector in order to observe undetected TeV gamma ray

  11. 44 (2007-5) Fig.1 Exploded view of a radial-flow-type combustor.

    E-Print Network [OSTI]

    Kasagi, Nobuhide

    44 (2007-5) Fig.1 Exploded view of a radial-flow-type combustor. On Catalyst Deployment for Uniform Temperature Distribution in Micro Ceramic Combustor * Takashi OKAMASA, Yuji SUZUKI and Nobuhide KASAGI catalytic combustor using high-precision ceramic tape-casting technology for micro thermophotovoltaic system

  12. New Dimensions of Visual Landscape Assessment Wildlands Management for Wildlife Viewing1

    E-Print Network [OSTI]

    Standiford, Richard B.

    preservation and other activities associated with traditional game management. Fortunately, much of the knowledge and techniques developed for game #12;management can be transferred to considerations of wildlifeNew Dimensions of Visual Landscape Assessment Wildlands Management for Wildlife Viewing1 Tamsie

  13. Message-embedding from a control-theoretical point of view

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Message-embedding from a control-theoretical point of view Gilles Mill´erioux1 , Jos´e Mar´ia Amig emphasis on one of the most attractive schemes, namely, message-embedding. The main conclusion can be stated as fol- lows: a message-embedded cryptosystem is equivalent to a conventional self

  14. Acid rain-the GEGB view G. D. HowellsandA. S. Kallend

    E-Print Network [OSTI]

    Short, Daniel

    Acid rain-the GEGB view G. D. HowellsandA. S. Kallend The article by John Franks (CJ'ern. -B conceh wirh acid rain follows thal policy In 1972.when Swedenput hs Case study on acid tain ro rhe United.Leatherhead,Sury Kr22 7SE. to acid rain,which does not justify th6 conlidenceshown by Franks. 'Acid in

  15. Multi-view Rendering using GPU for 3-D Displays Francois de Sorbier

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Multi-view Rendering using GPU for 3-D Displays Franc¸ois de Sorbier Graduate School of Science content for stereoscopic and auto-stereoscopic displays requires to render a scene several times from slightly different viewpoints. In that case, maintaining real-time rendering can be a difficult goal

  16. Nuclear Waste Disposal: An Independent View of the Big Picture and a Proposal for CARD

    E-Print Network [OSTI]

    California at Santa Cruz, University of

    1 Nuclear Waste Disposal: An Independent View of the Big Picture and a Proposal for CARD Presented to isolate nuclear waste successfully from the biosphere for the long term can be developed if our society to this impasse? In the 1940's at the beginning of the nuclear age, nuclear waste was seen as a "problem" only

  17. NEWS & VIEWS nature physics | VOL 3 | MARCH 2007 | www.nature.com/naturephysics 145

    E-Print Network [OSTI]

    Eggers, Jens

    by wetting -- the tendency of the liquid to spread on a material. Specifically, the critical speed that the flow, as viewed macroscopically, consists of a film of liquid racing along the upper surface of the sphere to close the void, and thus to produce a `plop'. Indeed, significant sound production depends

  18. Critical issues and current views on teaching basic circuits and systems in electrical engineering education

    E-Print Network [OSTI]

    education Joos Vandewalle Department of Electrical Engineering (ESAT) Katholieke Universiteit Leuven B-3001, its impact in the electrical engineering education and in order to increase the intrinsic interestCritical issues and current views on teaching basic circuits and systems in electrical engineering

  19. Reduced form electricity spot price modeling with a view towards spike risk

    E-Print Network [OSTI]

    Gerkmann, Ralf

    Reduced form electricity spot price modeling with a view towards spike risk Prof. Dr. Meyer. Februar 2010, 16:15 Uhr Seminarraum, Ludwigstraße 33 I The recent deregulation of electricity markets has led to the creation of energy exchanges, where the electricity is freely traded. We study the most

  20. Cauchy transforms of measures viewed as some functionals of Fourier transforms #

    E-Print Network [OSTI]

    Jurek, Zbigniew J.

    Cauchy transforms of measures viewed as some functionals of Fourier transforms # Zbigniew J. Jurek Urbanik ABSTRACT. The Cauchy transform of a positive measure plays an impor­ tant role in complex analysis and more recently in so­called free probability. We show here that the Cauchy transform restricted

  1. Cauchy transforms of measures viewed as some functionals of Fourier transforms

    E-Print Network [OSTI]

    Jurek, Zbigniew J.

    Cauchy transforms of measures viewed as some functionals of Fourier transforms Zbigniew J. Jurek Urbanik ABSTRACT. The Cauchy transform of a positive measure plays an impor- tant role in complex analysis and more recently in so-called free probability. We show here that the Cauchy transform restricted

  2. P2P Views Over Annotated Documents Konstantinos Karanasos, Ioana Manolescu

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    . Publication of such resources is inherently distributed. One could consider up- loading all published content] and social networks' tagging are among the most common methods to express annotations. Here, we designate and annotations. At the core of content sharing in AnnoVIP stand materialized views over the whole network content

  3. The Potential for Scholarly Online Books: Views from the Columbia University

    E-Print Network [OSTI]

    The Potential for Scholarly Online Books: Views from the Columbia University Online Books Evaluation Project* Mary Summerfield, Carol Mandel, and Paul Kantor The Columbia University Online Books Project, Columbia University: Final Report and many other reports and papers, including the re- search

  4. Replacement policies for shared caches on symmetric multicores : a programmer-centric point of view

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Replacement policies for shared caches on symmetric multicores : a programmer-centric point of view-cache replacement policy can be modified for self- performance to be meaningful. Categories and Subject Descriptors multicore processor, quality of service, self-per- formance, shared cache, replacement policy, memory band

  5. Distributed Energy Resources and Control: A power system point of view

    E-Print Network [OSTI]

    Distributed Energy Resources and Control: A power system point of view Oliver Gehrke, Stephanie. This paper gives an overview of the changes that the power system is undergoing and how these affect started in the early 1970s [1], many new tasks have been added to the workload of the power system

  6. PS3060: Perception and Action (L.2) A view from the cockpit of a fly

    E-Print Network [OSTI]

    Zanker, Johannes M.

    PS3060: Perception and Action (L.2) A view from the cockpit of a fly ­ the ecology of navigation · landmark navigation ... a universal problem ... !! perception and action !! the ecological approach perception : · perception happens in an ecological context: surfaces offer much more information than

  7. CSVT 05-03-17 1 4D Wavelet-Based Multi-view Video Coding

    E-Print Network [OSTI]

    Cai, Jianfei

    ) unify the view and temporal decompositions as a generic lifting transform. Secondly, an adaptive coding, high dimensional wavelet, lifting, global and local motion alignment. I. INTRODUCTION wide-viewpoint television, 3D television, immersive teleconference, surveillance, etc, are emerging. The existing

  8. Promoting the Benefits of Geospatial Data and Applications NebraskaView

    E-Print Network [OSTI]

    Nebraska-Lincoln, University of

    Promoting the Benefits of Geospatial Data and Applications NebraskaView Center for Advanced Land-Lincoln Geospatial Information for Nebraska Since the early 1970's, billions of dollars have been spent by the federal government and private sector on satellite-based earth observing systems. Hundreds of geospatial

  9. 3D Reconstruction of the Femoral Bone using two X-ray Images from Orthogonal Views

    E-Print Network [OSTI]

    3D Reconstruction of the Femoral Bone using two X-ray Images from Orthogonal Views B. Nikkhahe of the femur and 97 % of the model femur shaft less than 2 mm from the CT scan. Also the femoral head visualization of the femur including the femoral collumn and condyles is important for the clinician in a number

  10. Transmission System Expansion Plans in View Point of Deterministic, Probabilistic and Security Reliability Criteria

    E-Print Network [OSTI]

    Transmission System Expansion Plans in View Point of Deterministic, Probabilistic and Security control system as well as reasonable strength of grid originally. Because investment for power system the stability, and dynamic characteristics of the new system. A main reason of the separated work process

  11. Multiple Exciton Coherence Sizes in Photosynthetic Antenna Complexes viewed by Pump-Probe Spectroscopy

    E-Print Network [OSTI]

    Mukamel, Shaul

    Multiple Exciton Coherence Sizes in Photosynthetic Antenna Complexes viewed by Pump, 1996X The pump-probe signal from the light-harvesting antenna LH2 of purple bacteria is analyzed using absorption,4,5 pump-probe,11-16 and three pulse echoes.8,17 These measurements show ultrafast energy transfer

  12. Estimating amenity values of street trees and woodland views: a methodological review

    E-Print Network [OSTI]

    of spatial valuation methods. The review revealed a paucity of nationwide or large-scale valuations of street, and on trade-offs and synergies in ecosystem service provision. Objectives This research aimed to: m review methods and approaches to valuing amenity street trees and woodland views ­ and greenspace generally

  13. You are here: Home / News / 3D view of 1D nanostructures More services

    E-Print Network [OSTI]

    Espinosa, Horacio D.

    properties The nanowire battery Hybrid energy storage device is as small as it can possibly get ResearchersYou are here: Home / News / 3D view of 1D nanostructures Share More services Related LinksD nanostructures 09 January 2012 Nanowires show strong piezoelectricity Just 100 nanometers

  14. Optic for industrial endoscope/borescope with narrow field of view and low distortion

    DOE Patents [OSTI]

    Stone, Gary F.; Trebes, James E.

    2005-08-16T23:59:59.000Z

    An optic for the imaging optics on the distal end of a flexible fiberoptic endoscope or rigid borescope inspection tool. The image coverage is over a narrow (<20 degrees) field of view with very low optical distortion (<5% pin cushion or barrel distortion), compared to the typical <20% distortion. The optic will permit non-contact surface roughness measurements using optical techniques. This optic will permit simultaneous collection of selected image plane data, which data can then be subsequently optically processed. The image analysis will yield non-contact surface topology data for inspection where access to the surface does not permit a mechanical styles profilometer verification of surface topology. The optic allows a very broad spectral band or range of optical inspection. It is capable of spectroscopic imaging and fluorescence induced imaging when a scanning illumination source is used. The total viewing angle for this optic is 10 degrees for the full field of view of 10 degrees, compared to 40-70 degrees full angle field of view of the conventional gradient index or GRIN's lens systems.

  15. What if Extinction is Not Forever? A Molecular Paleontologist's View of De-extinction

    E-Print Network [OSTI]

    Archibald, S. Bruce

    want extinct animals to be brought back? Where would a de-extinct species end up living? How would its in response to major climate changes in the past, and why some species go extinct while others persistWhat if Extinction is Not Forever? A Molecular Paleontologist's View of De-extinction Presented

  16. Multiple Coordinated Views for Network Attack Graphs Steven Noel Michael Jacobs Pramod Kalapa Sushil Jajodia

    E-Print Network [OSTI]

    Noel, Steven

    Multiple Coordinated Views for Network Attack Graphs Steven Noel Michael Jacobs Pramod Kalapa graph-based representations have been developed for modeling combinations of low-level network attacks, relatively little attention has been paid to effective techniques for visualizing such attack graphs

  17. Articulated Mesh Animation from Multi-view Silhouettes Daniel Vlasic Ilya Baran Wojciech Matusik

    E-Print Network [OSTI]

    Popovic, Jovan

    , and deformation model learning. CR Categories: I.3.7 [Computer Graphics]: Three Dimensional Graphics and Realism--AnimationArticulated Mesh Animation from Multi-view Silhouettes Daniel Vlasic Ilya Baran Wojciech Matusik Institute of Technology Abstract Details in mesh animations are difficult to generate but they have great

  18. INFORMATION TECHNOLOGY INNOVATION: A VIEW OF LARGE CONTRACTORS1 T. Michael Toole, Member, ASCE

    E-Print Network [OSTI]

    Toole, T. Michael

    page 1 INFORMATION TECHNOLOGY INNOVATION: A VIEW OF LARGE CONTRACTORS1 T. Michael Toole, Member of technological innovations is whether diffusion is driven more by technology-push than by demand-pull mechanisms is to broaden the perspective on information technology (IT) innovation presented here at the congress

  19. NATURE CHEMISTRY | VOL 3 | NOVEMBER 2011 | www.nature.com/naturechemistry 839 news & views

    E-Print Network [OSTI]

    Chen, Peng

    NATURE CHEMISTRY | VOL 3 | NOVEMBER 2011 | www.nature.com/naturechemistry 839 news & views such as gasoline. The efficiency of FCC depends largely on catalysts. To improve the performance of these catalysts are inadequate for probing their catalytic performance. Now, writing in Nature Chemistry, Weckhuysen and co

  20. WindSat Polarimetric View of Greenland Peter Gaiser and 1

    E-Print Network [OSTI]

    Long, David G.

    WindSat Polarimetric View of Greenland 1 Li Li, 1 Peter Gaiser and 1 Elizabeth Twarog 1 Remote and observation geometry signatures, particularly over Greenland and Antarctic where the signatures are related-polarimetric microwavr radiometry; WindSat; snow; Greenland I. INTRODUCTION WindSat, launched in January 2003

  1. SeaWinds views Greenland Ivan S. Ashcraft and David G. Long

    E-Print Network [OSTI]

    Long, David G.

    SeaWinds views Greenland Ivan S. Ashcraft and David G. Long Brigham Young University, MERS facies in Greenland. The duration of the summer melt over the Greenland ice sheet is calculated using backscatter images of Greenland are produced to investigate diurnal varia- tions in backscatter measurements

  2. News and Views Conference Report: Paleoanthropology of the Mani Peninsula (Greece)

    E-Print Network [OSTI]

    Delson, Eric

    News and Views Conference Report: Paleoanthropology of the Mani Peninsula (Greece) K. Harvati & E in Athens and Areopolis, Greece, on the Paleoanthropol- ogy of the Mani Peninsula. The conference publication outside Greece is a short article on forensic implications of damage to one cranium (Coutselinis

  3. A multi-display viewing station that increases productivity and saves space

    E-Print Network [OSTI]

    Saskatchewan, University of

    A multi-display viewing station that increases productivity and saves space Conserve desk space) per monitor · Low-profile base saves desk space · Durable aluminum and steel construction creates that are VESA® MIS-D, 100/75, C compliant · Five-year warranty Product Sheet Space-saving desk stands Ergotron

  4. God and the Human Experience A physicist's case for a theistic world view

    E-Print Network [OSTI]

    Gea-Banacloche, Julio

    God and the Human Experience A physicist's case for a theistic world view Julio Gea-Banacloche 1, starting from the basic facts about the human experience of existence. For convenience (but also, when they, in turn, experience the need to look beyond the simple conception of God that saw them

  5. God and the human experience A physicist's case for a theistic world view

    E-Print Network [OSTI]

    Gea-Banacloche, Julio

    God and the human experience A physicist's case for a theistic world view Julio Gea-Banacloche 1 and--possibly--relate to that reality, by considering the various aspects of our (human beings') experience of existence. For convenience (but also with an ulterior theological purpose in mind

  6. Pathways for protein folding: is a "new view" needed? Vijay S Pande1

    E-Print Network [OSTI]

    Croquette, Vincent

    Pathways for protein folding: is a "new view" needed? Vijay S Pande1 , Alexander Yu Grosberg2 energy MG Molten globule Introduction How do proteins fold? While the thirty five years since Anfinsen has demonstrated the complexity of protein folding, the search continues for the general principles

  7. NATURE CHEMISTRY | VOL 3 | SEPTEMBER 2011 | www.nature.com/naturechemistry 659 news & views

    E-Print Network [OSTI]

    Sia, Samuel K.

    ion (uranium). The use of chemistry for improving sensors is particularly inspired. Current workNATURE CHEMISTRY | VOL 3 | SEPTEMBER 2011 | www.nature.com/naturechemistry 659 news & views D if analogous enzymes are available for the analyte of interest. Writing in Nature Chemistry, Yi Lu and Yu Xiang

  8. ---Search Finance Home Yahoo! Help Welcome, dalemeade08540 [ ]Sign Out -My Yahoo! View Customize

    E-Print Network [OSTI]

    - - -Search Finance Home Yahoo! Help Welcome, dalemeade08540 [ ]Sign Out -My Yahoo! View Customize Financial News Enter symbol(s) Basic Get Symbol Lookup Associated Press EU Expected to Offer Japan Bigger, Russia, South Korea and China plan to join forces to research uses of fusion energy to create clean, long-term

  9. VIEW OFPASSAIC FALLS by W. H. Bartlett, 1840 FOSSIL GREAT LAKES

    E-Print Network [OSTI]

    Olsen, Paul E.

    to Field Trips,52nd Ann. Mtg.New York State Geology Association, Newark College of Arts and SciencesVIEW OFPASSAIC FALLS by W. H. Bartlett, 1840 FOSSIL GREAT LAKES OF THE NEWARK SUPERGROUP IN NEW JERSEY Paul E. Olsen From: W. Manspeizer (ed.),1980,Field Studies in New Jersey Geology and Guide

  10. Automated RF Testing of the KU Agile Radio 2.0 Using LabView

    E-Print Network [OSTI]

    Kansas, University of

    the command line in LabVIEW, writing it to an external command file, and then calling an external ssh client for the transmitter is set by issuing commands to an MC68HC08 microcontroller which in turn sets the control voltage in Figure 1, the micro controller inputs can be seen as blue lines entering the top of the diagram. The 6

  11. Theory of Evolutionary Algorithms: A Birds Eye View A. E. Eibena

    E-Print Network [OSTI]

    Rudolph, Günter

    Theory of Evolutionary Algorithms: A Birds Eye View A. E. Eibena and G. Rudolphb a Leiden Institute the readers' orientation in evolu- tionary computation theory. In the meanwhile, this survey provides key as a survival task: possible solutions compete with each other for survival (and the right to reproduce

  12. A CSP View on UMLRT structure diagrams Clemens Fischer, ErnstRudiger Olderog and Heike Wehrheim

    E-Print Network [OSTI]

    Habel, Annegret

    A CSP View on UML­RT structure diagrams Clemens Fischer, Ernst­R¨udiger Olderog and Heike Wehrheim UML­RT structure diagrams together with the formal method CSP­OZ combining CSP and Object­Z. While CSP­OZ is used for specifying the system components themselves (by CSP­OZ classes), UML­RT diagrams provide

  13. Transform-Space View: Performing Spatial Join in the Transform Space

    E-Print Network [OSTI]

    Whang, Kyu-Young

    Transform-Space View: Performing Spatial Join in the Transform Space Using Original-Space Indexes that satisfy a given spatial relationship. In spatial joins using indexes, original-space indexes such as the R-tree are widely used. An original-space index is the one that indexes objects as represented in the original space

  14. Faculty of Engineering Courses click on a course to view details

    E-Print Network [OSTI]

    Mottram, Nigel

    ENGINEERING MSc/PgDip 37 MARINE TECHNOLOGY MSc/PgDip/PgCert 04 MECHANICAL ENGINEERING, ADVANCED MSc/PgDip 39 URBAN DESIGN MSc/PgDip/PgCert 08 WIND ENERGY SYSTEMS MSc 33 #12;FACULTY OF ENGINEERING 2 back1 Faculty of Engineering Courses click on a course to view details FACULTY OF ENGINEERING COURSE

  15. Toward Structural Dynamics: Protein Motions Viewed by Chemical Shift Modulations and Direct Detection of CN

    E-Print Network [OSTI]

    Toward Structural Dynamics: Protein Motions Viewed by Chemical Shift Modulations and Direct of anti-correlated fluctuations) in R-helices. This extends the prospects of structure- dynamics, several groups have proposed to investigate the existence of structure-dynamics relationships. We like

  16. Protein structure similarity based on multi-view images generated from 3D molecular visualization

    E-Print Network [OSTI]

    Fukui, Kazuhiro

    to protein structure comparison using canonical angles between two subspaces generated from multiple views extension alignment and the Gauss integral tuning. 1. Introduction In structural biology, finding representation of 3D protein structure as a 31- dimensional feature vector called Gauss Integral Tuning (GIT) [6

  17. Catching the Best Views of Skyline: A Semantic Approach Based on Decisive Subspaces

    E-Print Network [OSTI]

    Pei, Jian

    Catching the Best Views of Skyline: A Semantic Approach Based on Decisive Subspaces Jian Pei1 Wen of skylines remains open: Why and in which subspaces is (or is not) an ob- ject in the skyline? Practically-up and drill- down analysis is introduced. We also develop an efficient algorithm, Skyey, to compute

  18. Gene Calling Standards (GSC8 Meeting)

    ScienceCinema (OSTI)

    Kyrpides, Nikos [Genome Biology Program, DOE JGI

    2011-04-28T23:59:59.000Z

    The Genomic Standards Consortium was formed in September 2005. It is an international, open-membership working body which promotes standardization in the description of genomes and the exchange and integration of genomic data. The 2009 meeting was an activity of a five-year funding "Research Coordination Network" from the National Science Foundation and was organized held at the DOE Joint Genome Institute with organizational support provided by the JGI and by the University of California - San Diego. Nikos Kyrpides of the DOE Joint Genome Institute discusses gene calling standards at the Genomic Standards Consortium's 8th meeting at the DOE JGI in Walnut Creek, Calif. on Sept. 10, 2009.

  19. Engineering disease resistance with pectate lyase-like genes

    DOE Patents [OSTI]

    Vogel, John; Somerville, Shauna

    2005-03-08T23:59:59.000Z

    A mutant gene coding for pectate lyase and homologs thereof is provided, which when incorporated in transgenic plants effect an increased level disease resistance in such plants. Also is provided the polypeptide sequence for the pectate lyase of the present invention. Methods of obtaining the mutant gene, producing transgenic plants which include the nucleotide sequence for the mutant gene and producing improved disease resistance in a crop of such transgenic plants are also provided.

  20. Petascale Debugging via Allinea DDT for IBM Blue Gene /P

    E-Print Network [OSTI]

    Kemner, Ken

    Petascale Debugging via Allinea DDT for IBM Blue Gene /P and IBM Blue Gene /Q Ian Lumb petascaling Allinea DDT Petascaling Allinea DDT for IBM Blue Gene /x Getting Started with Allinea DDT #12;0 50,000 100,000 150,000 200,000 0 0.02 0.04 0.06 0.08 0.1 0.12 DDT 3.0 Performance Figures Jaguar Cray

  1. was nonrandom, localized to genes and within particular regions of genes, much like sense tran-

    E-Print Network [OSTI]

    Xie, Xiaoliang Sunney

    number of genes. References and Notes 1. P. O. Brown, D. Botstein, Nat. Genet. 21, 33 (1999). 2. V. E (2002). 10. B. Lehner, G. Williams, R. D. Campbell, C. M. Sanderson, Trends Genet. 18, 63 (2002). 11. J Acids Res. 32, 4812 (2004). 9. M. E. Fahey, T. F. Moore, D. G. Higgins, Comp. Funct. Genomics 3, 244

  2. Candidate genes affecting Drosophila life span identified by integrating microarray gene expression analysis and QTL mapping

    E-Print Network [OSTI]

    Mackay, Trudy F.C.

    University, Boston, MA 02111, United States b Department of Genetics and W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC 27695-7614, United States Received 4 April 2006, short-lived animal models are essential to identifying the mechanisms and genes that affect the rate

  3. acquired resistance genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and fractionation resistance Biology and Medicine Websites Summary: in Paramecium, Gout et al. 10 identify a clear relationship between high WGD duplicate gene retention...

  4. adenovirus early gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Kupffer cell activation and viral gene expression in early liver toxicity after infusion of recombinant adenovirus vectors CiteSeer Summary: Systemic application of...

  5. autoimmunity susceptibility gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    curable by the removal of culpable antibodies and antigens. 1. unknown authors 5 The Dyslexia Susceptibility Genes and Neurodevelopment. Open Access Theses and Dissertations...

  6. autism susceptibility genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the susceptibility for autism. To this end, 267 subjects with autism spectrum 3 The Dyslexia Susceptibility Genes and Neurodevelopment. Open Access Theses and Dissertations...

  7. arthritis susceptibility genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    worldwide will be able to access these treatments. Yuti Chernajovsky 2005-01-01 7 The Dyslexia Susceptibility Genes and Neurodevelopment. Open Access Theses and Dissertations...

  8. Mutator gene and hereditary non-polyposis colorectal cancer

    DOE Patents [OSTI]

    de la Chapelle, Albert (Helsingfors, FI); Vogelstein, Bert (Baltimore, MD); Kinzler, Kenneth W. (Baltimore, MD)

    2008-02-05T23:59:59.000Z

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error.sup.+ (RER.sup.+) tumor cells.

  9. acid gene cluster: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the application of a novel clustering algorithm, Super-Paramagnetic Clustering (SPC) to analysis of gene expression profiles that were generated recently during a study of...

  10. apoptosis gene profiling: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the application of a novel clustering algorithm, Super-Paramagnetic Clustering (SPC) to analysis of gene expression profiles that were generated recently during a study of...

  11. arom gene cluster: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the application of a novel clustering algorithm, Super-Paramagnetic Clustering (SPC) to analysis of gene expression profiles that were generated recently during a study of...

  12. almt1 gene cluster: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the application of a novel clustering algorithm, Super-Paramagnetic Clustering (SPC) to analysis of gene expression profiles that were generated recently during a study of...

  13. Genomics, Gene Expression and Other Studies in Soybean Rust

    E-Print Network [OSTI]

    Posada-Buitrago, Martha Lucia

    2005-01-01T23:59:59.000Z

    Joint Genome Institute Genomics, Gene Expression and otherRust Martha Lucía Posada-Buitrago Ph.D Genomics DivisionEvolutionary Genomics DOE- Joint Genome Institute Lawrence

  14. aurata hemoglobin genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    chain reaction and in situ hybridization, the expression of the prolactin (PRL) gene was determined during development in gilthead sea bream (Sparus aurata) for the...

  15. aspartyl proteinase gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and pathogen Edinburgh, University of - Research Archive Summary: Parasite nematode genomics is a relatively new field9, but already two of the most interesting gene families to...

  16. GENE 7360: Teaching Internship in Genetics Instructor: Rodney Mauricio

    E-Print Network [OSTI]

    Arnold, Jonathan

    GENE 7360: Teaching Internship in Genetics Instructor: Rodney Mauricio Fall 2004 COURSE DESCRIPTION to serve as a formal internship in the teaching of Genetics. Through close faculty supervision

  17. Shaky emerging economies in view of the global financial crisis: The Turkish economy after three decades of liberal reforms

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    into the heart of the 2007/08 crisis yet, their structural vulnerabilities continue to haunt the futureShaky emerging economies in view of the global financial crisis: The Turkish economy after three2012 #12;1 Shaky emerging economies in view of the global financial crisis: The Turkish economy after

  18. Global trade in dairy products involves complex and con-tentious issues. Viewed broadly from the perspective of

    E-Print Network [OSTI]

    Radeloff, Volker C.

    Global trade in dairy products involves complex and con- tentious issues. Viewed broadly from the perspective of "society," there are large benefits from liberalizing trade. Viewed narrowly from the perspective of dairy producers, liberalized trade is desirable only if it expands exports and reduces imports

  19. Integrating Web information with XML Concrete Views Tuyt-Trm Dang-Ngoc Huaizhong Kou Georges Gardarin

    E-Print Network [OSTI]

    Boyer, Edmond

    Integrating Web information with XML Concrete Views Tuyêt-Trâm Dang-Ngoc Huaizhong Kou Georges.Lastname}@prism.uvsq.fr ABSTRACT To cope with the difficulties of Web information search, lots of technologies related to Web another Web search engines with general purpose, this paper couples text mining and XML view caching

  20. The thick black line: An analysis of police officer views on racial profiling and the police-minority relationship

    E-Print Network [OSTI]

    Glover, Karen Suzanne

    2004-09-30T23:59:59.000Z

    Much research has been conducted on the police-minority relationship, primarily from the view of the civilian. As central actors, the police view is noticeably absent from the research. I employed in-depth interviews and open-ended survey...

  1. Remote Use of the SOAR 4.25m Telescope with LabVIEW Gerald Cecil*a

    E-Print Network [OSTI]

    Cecil, Gerald

    Remote Use of the SOAR 4.25m Telescope with LabVIEW Gerald Cecil*a , Adam Crain**a , Gérman at all U.S. research universities will be able to use it remotely, avoiding 24+ hrs of travel developed LabVIEW modules for remote observing that minimize bandwidth to the shared LAN atop Cerro Pachón

  2. How to View Clips in the Video Clip Library: Copy and paste the following link into your web browser.

    E-Print Network [OSTI]

    Qiu, Weigang

    1 How to View Clips in the Video Clip Library: Copy and paste the following link into your." This will open a new list of links, in the navigation panel on the left. Click on "Video Analysis of Teaching". The screen to the right is the VAT homepage. In order to upload or view the lesson video that you have

  3. PPPL-3187 -Preprint: May 1996, UC-420, 426 A Procedure for Generating Quantitative 3-D Camera Views

    E-Print Network [OSTI]

    . Introduction The very high heat loads on the TPX divertor targets require continuous temperature monitoring monitoring tokamak internal components, particularly high heat flux divertor components. These models for reproducing these views. An analytic calculation of the resolution and viewing incidence angle is developed

  4. PPPL3187 Preprint: May 1996, UC420, 426 A Procedure for Generating Quantitative 3D Camera Views

    E-Print Network [OSTI]

    . Introduction The very high heat loads on the TPX divertor targets require continuous temperature monitoring monitoring tokamak internal components, particularly high heat flux divertor components. These models for reproducing these views. An analytic calculation of the resolution and viewing incidence angle is developed

  5. Variant translocation of the bcl-2 gene to immunoglobulin. lambda. light chain gene in chronic lymphocytic leukemia

    SciTech Connect (OSTI)

    Adachi, M.; Cossman, J.; Longo, D.; Croce, C.M.; Tsujimoto, Y. (Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA))

    1989-04-01T23:59:59.000Z

    The bcl-2 gene has been identified as a gene directly involved in the consistent chromosome translocation t(14;18), which is found in {approx} 90% of human follicular lymphoma cases, and is a prime candidate for the oncogene playing a crucial role in follicular lymphomagenesis. In this paper, the authors describe a case of chronic lymphocytic leukemia showing the juxtaposition of the bcl-2 gene on chromosome 18 to immunoglobulin {lambda} light chain (Ig{lambda}) gene on chromosome 22 in a head-to-head configuration. Sequencing analysis of the joining site of the bcl-2 gene and Ig{lambda} gene has shown that the breakpoint is within the 5{prime} flanking region of the bcl-2 gene and about 2.2 kilobases 5{prime} to the joining segment of Ig{lambda} locus in a germ-line configuration. The extranucleotide, commonly appearing at the joining site of the t(14;18) translocation involving the IgH locus, is absent from the joining site of bcl-2 and Ig{lambda}. The lack of extranucleotide suggests that the juxtaposition of the bcl-2 and Ig{lambda} genes occurred during physiological rearrangement of the Ig{lambda} gene since it has been shown that the rearrangement of the Ig{lambda} locus is not accompanied by extranucleotides.

  6. Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph

    SciTech Connect (OSTI)

    Daniel J Arp

    2005-06-15T23:59:59.000Z

    Nitrosomonas europaea is an aerobic lithoautotrophic bacterium that uses ammonia (NH3) as its energy source. As a nitrifier, it is an important participant in the nitrogen cycle, which can also influence the carbon cycle. The focus of this work was to explore the genetic structure and mechanisms underlying the lithoautotrophic growth style of N. europaea. Whole genome gene expression. The gene expression profile of cells in exponential growth and during starvation was analyzed using microarrays. During growth, 98% of the genes increased in expression at least two fold compared to starvation conditions. In growing cells, approximately 30% of the genes were expressed eight fold higher, Approximately 10% were expressed more than 15 fold higher. Approximately 3% (91 genes) were expressed to more than 20 fold of their levels in starved cells. Carbon fixation gene expression. N. europaea fixes carbon via the Calvin-Benson-Bassham (CBB) cycle via a type I ribulose bisphosphate carboxylase/oxygenase (RubisCO). This study showed that transcription of cbb genes was up-regulated when the carbon source was limited, while amo, hao and other energy harvesting related genes were down-regulated. Iron related gene expression. Because N. europaea has a relatively high content of hemes, sufficient Fe must be available in the medium for it to grow. The genome revealed that approximately 5% of the coding genes in N. europaea are dedicated to Fe transport and assimilation. Nonetheless, with the exception of citrate biosynthesis genes, N. europaea lacks genes for siderophore production. The Fe requirements for growth and the expression of the putative membrane siderophore receptors were determined. The N. europaea genome has over 100 putative genes ({approx}5% of the coding genes) related to Fe uptake and its siderophore receptors could be grouped phylogenetically in four clusters. Fe related genes, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin, and diffusion protein OmpC were expressed to higher levels under Fe limitation. N. europaea has a high Fe requirement and under Fe limiting conditions (0.2 ?M), is capable to assimilate up to 70% of the available Fe without the ability to produce siderophores.

  7. Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph

    SciTech Connect (OSTI)

    Daniel J. Arp

    2005-05-25T23:59:59.000Z

    Nitrosomonas europaea is an aerobic lithoautotrophic bacterium that uses ammonia (NH3) as its energy source. As a nitrifier, it is an important participant in the nitrogen cycle, which can also influence the carbon cycle. The focus of this work was to explore the genetic structure and mechanisms underlying the lithoautotrophic growth style of N. europaea. Whole genome gene expression: The gene expression profile of cells in exponential growth and during starvation was analyzed using microarrays. During growth, 98% of the genes increased in expression at least two fold compared to starvation conditions. In growing cells, approximately 30% of the genes were expressed eight fold higher, Approximately 10% were expressed more than 15 fold higher. Approximately 3% (91 genes) were expressed to more than 20 fold of their levels in starved cells. Carbon fixation gene expression: N. europaea fixes carbon via the Calvin-Benson-Bassham (CBB) cycle via a type I ribulose bisphosphate carboxylase/oxygenase (RubisCO). This study showed that transcription of cbb genes was up-regulated when the carbon source was limited, while amo, hao and other energy harvesting related genes were down-regulated. Iron related gene expression: Because N. europaea has a relatively high content of hemes, sufficient Fe must be available in the medium for it to grow. The genome revealed that approximately 5% of the coding genes in N. europaea are dedicated to Fe transport and assimilation. Nonetheless, with the exception of citrate biosynthesis genes, N. europaea lacks genes for siderophore production. The Fe requirements for growth and the expression of the putative membrane siderophore receptors were determined. The N. europaea genome has over 100 putative genes ({approx}5% of the coding genes) related to Fe uptake and its siderophore receptors could be grouped phylogenetically in four clusters. Fe related genes, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin, and diffusion protein OmpC were expressed to higher levels under Fe limitation. N. europaea has a high Fe requirement and under Fe limiting conditions (0.2 {micro}M), is capable to assimilate up to 70% of the available Fe without the ability to produce siderophores.

  8. NATURE METHODS | VOL.9 NO.11 | NOVEMBER 2012 | 1061 NEWS AND VIEWS

    E-Print Network [OSTI]

    Khalil, Ahmad S.

    organizing principles of life and have the potential to transform industries including medicine, agriculture and energy. Organisms can be programmed by combining genetically encoded parts, such as transcrip- tion the active and repressed states) and the ability to be composed upstream of a single gene for multi-input

  9. nature methods | VOL.9 NO.11 | NOVEMBER 2012 | 1061 news and views

    E-Print Network [OSTI]

    Cai, Long

    organizing principles of life and have the potential to transform industries including medicine, agriculture and energy. Organisms can be programmed by combining genetically encoded parts, such as transcrip- tion the active and repressed states) and the ability to be composed upstream of a single gene for multi-input

  10. Interferon-Stimulated Genes in the Pregnant Mouse Uterus

    E-Print Network [OSTI]

    Tilford, Sarah

    2008-08-24T23:59:59.000Z

    INTERFERON-STIMULATED GENES IN THE PREGNANT MOUSE UTERUS A Senior Honors Thesis by SARAH TILFORD Submitted to the Office of Honors Programs Texas A&M University In partial... fulfillment of the requirements of the UNIVERSITY UNDERGRADUATE RESEARCH FELLOWS May 2008 Major: Biomedical Science and Chemistry ii ABSTRACT Interferon-Stimulated Genes in the Pregnant Mouse Uterus (May 2008) Sarah Tilford Department...

  11. Kinetics of gene derepression by ERK signaling , Nria Samperb

    E-Print Network [OSTI]

    Shvartsman, Stanislav "Stas"

    Kinetics of gene derepression by ERK signaling Bomyi Lima , Núria Samperb , Hang Luc , Christine February 25, 2013) ERK controls gene expression in development, but mechanisms that link ERK activation to study transcriptional interpretation of ERK signaling during Dro- sophila embryogenesis, at a stage when

  12. Uncorrected Proof Copy Finding Genes by Using Computational Tools 85

    E-Print Network [OSTI]

    Uncorrected Proof Copy Finding Genes by Using Computational Tools 85 Job: Plant Functional Genomics/Rev.02.03 Uncorrected Proof Copy #12;Uncorrected Proof Copy Finding Genes by Using Computational Tools 87 Computational Tools 89 Job: Plant Functional Genomics--Grotewold Compositor: Nettype Chapter: Chapter 6 Date: 3

  13. RESEARCH Open Access Transient receptor potential genes, smoking,

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    RESEARCH Open Access Transient receptor potential genes, smoking, occupational exposures and cough chemicals and may play an important role in the pathogenesis of cough. The aim was to study the influence of single nucleotide polymorphisms (SNPs) in TRP genes and irritant exposures on cough. Methods: Nocturnal

  14. Visualization of Gene Combinations Christian Tominski & Heidrun Schumann

    E-Print Network [OSTI]

    Tominski, Christian

    expression or gene regulation) in different experi- ments or at different time steps. A variety of approaches- ing combinations for further investigation. Our approach manifests in an analysis pipeline, we describe the follow- ing concepts in more detail: · a pipeline for the visual analysis of gene

  15. INTRODUCTION Cascades of gene interactions are critical for the establishment

    E-Print Network [OSTI]

    Quake, Stephen R.

    along the anterior- posterior axis, predominantly where cuticle defects are seen in embryos that lack homeotic proteins control animal development. In particular, the basis for differential regulation of target genes must be learned. Target genes can be activated by some homeotic proteins and repressed

  16. Identification of Human Gene Core Promoters Michael Q. Zhang1

    E-Print Network [OSTI]

    Identification of Human Gene Core Promoters in Silico Michael Q. Zhang1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 USA Identification of the 5 -end of human genes requires--CorePromoter. Our experiments indicate that when given a 1- to 2-kb extended promoter, CorePromoter will correctly

  17. GenePRIMP: A software quality control tool

    SciTech Connect (OSTI)

    Amrita Pati

    2010-05-05T23:59:59.000Z

    Amrita Pati of the DOE Joint Genome Institute's Genome Biology group describes the software tool GenePRIMP and how it fits into the quality control pipeline for microbial genomics. Further details regarding GenePRIMP appear in a paper published online May 2, 2010 in Nature Methods.

  18. Hotspots of Biased Nucleotide Substitutions in Human Genes

    E-Print Network [OSTI]

    Sorenson, Michael

    Hotspots of Biased Nucleotide Substitutions in Human Genes Jonas Berglund1 , Katherine S. Pollard2) Hotspots of biased nucleotide substitutions in human genes. PLoS Biol 7(1): e1000026. doi:10.1371/journal selection in the human lineage. However, HARs tend to have biased patterns of nucleotide substitution

  19. GenePRIMP: A software quality control tool

    ScienceCinema (OSTI)

    Amrita Pati

    2010-09-01T23:59:59.000Z

    Amrita Pati of the DOE Joint Genome Institute's Genome Biology group describes the software tool GenePRIMP and how it fits into the quality control pipeline for microbial genomics. Further details regarding GenePRIMP appear in a paper published online May 2, 2010 in Nature Methods.

  20. Photoacoustic microscopy of tyrosinase reporter gene in vivo

    E-Print Network [OSTI]

    Wang, Lihong

    Photoacoustic microscopy of tyrosinase reporter gene in vivo Arie Krumholz Sarah J. Van microscopy of tyrosinase reporter gene in vivo Arie Krumholz,a Sarah J. VanVickle-Chavez,b Junjie Yao for tyrosinase, the primary enzyme responsible for expression of melanin in melanogenic cells. Optical res

  1. Inferring Gene Family Histories in Yeast Identifies Lineage Specific Expansions

    E-Print Network [OSTI]

    Ames, Ryan M.; Money, Daniel; Lovell, Simon C.

    2014-06-12T23:59:59.000Z

    new evolutionary models to infer gene family histories across complete yeast genomes; these models allow us to estimate the relative genome-wide rates of gene birth, death, innovation and extinction (loss of an entire family) for the first time. We...

  2. HumanMouse Gene Identification by Comparative Evidence Integration and

    E-Print Network [OSTI]

    Pavlovic, Vladimir

    The identification of genes in the human genome remains a challenge, as the actual predictions appear to disagree of genes in the human genome by using a reference, such as mouse genome. However, this comparative genome. In particular, it is not clear whether the mouse is at the correct evolutionary distance from

  3. Lung Disease in Pediatrics: is it all in the Genes?

    E-Print Network [OSTI]

    Lung Disease in Pediatrics: is it all in the Genes? Jay K. Kolls, M.D.Jay K. Kolls, M.D. Chair with CF do worse than other? #12;· Outcomes are better at CF centers · There is huge variation in lung with the same mutation do worse than others? · Modifier genes ­ lung disease ­ Tgfb1 ­ Irfd1 ­ neutrophil

  4. Diversity of gene expression in adenocarcinoma of the lung

    E-Print Network [OSTI]

    Botstein, David

    Diversity of gene expression in adenocarcinoma of the lung Mitchell E. Garber*, Olga G. Troyanskaya Contributed by David Botstein, September 21, 2001 The global gene expression profiles for 67 human lung tumors pathologic analysis. Four main histologic subtypes of lung cancer are regularly distinguished by tumor

  5. Basin View Geothermal Heating District, Klamath Falls, Oregon: conceptual design and economic-feasibility study report

    SciTech Connect (OSTI)

    Not Available

    1981-07-01T23:59:59.000Z

    The findings of a feasibility study performed for Basin View Heating District in Klamath Falls, Oregon are reported. The purpose of the study is to determine the physical, economic, and political feasibility of establishing a geothermal heating district to provide space heat to housing units in the Basin View Development of Klamath Falls. Of the several systems considered, all are physically feasible. The project is politically feasible if the owner compiles with governmental requirements. Economic feasibility is based on considerations of money value rates, tax rates and expected rates of return, which are dependent on government and money markets. For analysis a money value rate of 21% and an owner's marginal tax rate of 35% were adopted.

  6. Mutational analysis of the lambda S gene

    E-Print Network [OSTI]

    Neal, Gregory Scott

    1984-01-01T23:59:59.000Z

    , the early stages of gene expression 1n the lytic cycle are not germane to this d1scussion. Late protein synthes1s is programmed from transcr1pts initiated at a single promoter, Pr'. This promoter is controlled by termination at the term1nator trz, and 1t... p JG13 pJH2 pZ150 pZ152 pGN200 pGN201 CSH57B met+ lacIQI lacZ+ ~tr + su- CQ21 F'~ro lacIQI lacZ Tn5 (PI) F'tra036, proAB+, lacZm15, lacI/ a~lac ro, thi, strtr, endA, ~scB15, , ~su E AmpR TetR lacPO (in pBR322) AmpR lacPO Sam7 R+Rz+ (in p...

  7. Prediction of epigenetically regulated genes in breast cancer cell lines

    SciTech Connect (OSTI)

    Loss, Leandro A; Sadanandam, Anguraj; Durinck, Steffen; Nautiyal, Shivani; Flaucher, Diane; Carlton, Victoria EH; Moorhead, Martin; Lu, Yontao; Gray, Joe W; Faham, Malek; Spellman, Paul; Parvin, Bahram

    2010-05-04T23:59:59.000Z

    Methylation of CpG islands within the DNA promoter regions is one mechanism that leads to aberrant gene expression in cancer. In particular, the abnormal methylation of CpG islands may silence associated genes. Therefore, using high-throughput microarrays to measure CpG island methylation will lead to better understanding of tumor pathobiology and progression, while revealing potentially new biomarkers. We have examined a recently developed high-throughput technology for measuring genome-wide methylation patterns called mTACL. Here, we propose a computational pipeline for integrating gene expression and CpG island methylation profles to identify epigenetically regulated genes for a panel of 45 breast cancer cell lines, which is widely used in the Integrative Cancer Biology Program (ICBP). The pipeline (i) reduces the dimensionality of the methylation data, (ii) associates the reduced methylation data with gene expression data, and (iii) ranks methylation-expression associations according to their epigenetic regulation. Dimensionality reduction is performed in two steps: (i) methylation sites are grouped across the genome to identify regions of interest, and (ii) methylation profles are clustered within each region. Associations between the clustered methylation and the gene expression data sets generate candidate matches within a fxed neighborhood around each gene. Finally, the methylation-expression associations are ranked through a logistic regression, and their significance is quantified through permutation analysis. Our two-step dimensionality reduction compressed 90% of the original data, reducing 137,688 methylation sites to 14,505 clusters. Methylation-expression associations produced 18,312 correspondences, which were used to further analyze epigenetic regulation. Logistic regression was used to identify 58 genes from these correspondences that showed a statistically signifcant negative correlation between methylation profles and gene expression in the panel of breast cancer cell lines. Subnetwork enrichment of these genes has identifed 35 common regulators with 6 or more predicted markers. In addition to identifying epigenetically regulated genes, we show evidence of differentially expressed methylation patterns between the basal and luminal subtypes. Our results indicate that the proposed computational protocol is a viable platform for identifying epigenetically regulated genes. Our protocol has generated a list of predictors including COL1A2, TOP2A, TFF1, and VAV3, genes whose key roles in epigenetic regulation is documented in the literature. Subnetwork enrichment of these predicted markers further suggests that epigenetic regulation of individual genes occurs in a coordinated fashion and through common regulators.

  8. Conrad Aiken's manipulation of point of view in selected short stories

    E-Print Network [OSTI]

    Abbe, Margaret Ann

    1975-01-01T23:59:59.000Z

    thank Dr. Harry Kroitor for his encouragement in the preparation of this thesis and his guidance in the understanding of point of view. Finally, I went to thank my husband and my parents for their encouragement, support, and patience. viii TABIZ... of tone. Most criticisms briefly explicate Aiken'8 short stories or deal with symbolic meanings within them. Frederick Hoffman, for example, explicates several of Aiken's stories in his book Conrad Aiken and 10 also deals briefly with symbolic meaning...

  9. Private Narratives and Infant Views: Iconizing 1970s Militancy in Contemporary Argentine Cinema

    E-Print Network [OSTI]

    Garibotto, Veró nica

    2015-01-01T23:59:59.000Z

    representation, and intensifies iconicity. Or, to put it in semiotic terms, the symbolic and the indexical dimensions contribute to the process of iconization, inhibiting new affective configurations. The repetition of historically- 15 marked social...1 Private Narratives and Infant Views: Iconizing 1970s Militancy in Contemporary Argentine Cinema Shot in 2011 and released in September 2012, Infancia clandestina, by Argentine director Benjamín Ávila, has already been sold to over twenty...

  10. THE HISTORY OF US PRESIDENTIAL ELECTIONS FROM SIBERIAH HC POIHT OF VIEW

    E-Print Network [OSTI]

    Gorban, Alexander N.

    THE HISTORY OF US PRESIDENTIAL ELECTIONS FROM SIBERIAH HC POIHT OF VIEW Cory Waxman Krasnoyarsk, Krasnoyarsk-36, Russia Az. 8 5 0 2 0 U. S. A. Tests were performed with the program "US Presidential Elections systems, and may permit i t t o t r a i n tens of thousands of times f a s t e r i n c e r t a i n

  11. Assessment of industry views on international business prospects for solar thermal technology

    SciTech Connect (OSTI)

    Easterling, J.C.

    1984-09-01T23:59:59.000Z

    This report contains a review of solar thermal industry viewpoints on their prospects for developing international business. The report documents the industry's current involvement in foreign markets, view of foreign competition in overseas applications, and view of federal R and D and policy requirements to strengthen international business prospects. The report is based on discussions with equipment manufacturers and system integrators who have a product or service with potential international demand. Interviews with manufacturers and system integrators were conducted by using a standard format for interview questions. The use of a standard format for questions provided a basis for aggregating similar views expressed by US companies concerning overseas business prospects. A special effort was made to gather responses from the entire solar thermal industry, including manufacturers of line-focus, point-focus, and central receiver systems. General, technical, economic, institutional, and financial findings are provided in this summary. In addition, Pacific Northwest Laboratory (PNL) recommendations are provided (based upon advice from the Solar Thermal Review Panel) for activities to improve US solar thermal business prospects overseas.

  12. Extensive horizontal gene transfer, duplication, and loss of chlorophyll synthesis genes in the algae

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hunsperger, Heather M.; Randhawa, Tejinder; Cattolico, Rose Ann

    2015-01-01T23:59:59.000Z

    Two non-homologous, isofunctional enzymes catalyze the penultimate step of chlorophyll a synthesis in oxygenic photosynthetic organisms such as cyanobacteria, eukaryotic algae and land plants: the light independent (LIPOR) and light-dependent (POR) protochlorophyllide oxidoreductases. Whereas the distribution of these enzymes in cyanobacteria and land plants is well understood, the presence, loss, duplication, and replacement of these genes have not been surveyed in the polyphyletic and remarkably diverse eukaryotic algal lineages.

  13. Genomic Analyses of Bacterial Porin-Cytochrome Gene Clusters

    SciTech Connect (OSTI)

    Shi, Liang; Fredrickson, Jim K.; Zachara, John M.

    2014-11-26T23:59:59.000Z

    The porin-cytochrome (Pcc) protein complex is responsible for trans-outer membrane electron transfer during extracellular reduction of Fe(III) by the dissimilatory metal-reducing bacterium Geobacter sulfurreducens PCA. The identified and characterized Pcc complex of G. sulfurreducens PCA consists of a porin-like outer-membrane protein, a periplasmic 8-heme c type cytochrome (c-Cyt) and an outer-membrane 12-heme c-Cyt, and the genes encoding the Pcc proteins are clustered in the same regions of genome (i.e., the pcc gene clusters) of G. sulfurreducens PCA. A survey of additionally microbial genomes has identified the pcc gene clusters in all sequenced Geobacter spp. and other bacteria from six different phyla, including Anaeromyxobacter dehalogenans 2CP-1, A. dehalogenans 2CP-C, Anaeromyxobacter sp. K, Candidatus Kuenenia stuttgartiensis, Denitrovibrio acetiphilus DSM 12809, Desulfurispirillum indicum S5, Desulfurivibrio alkaliphilus AHT2, Desulfurobacterium thermolithotrophum DSM 11699, Desulfuromonas acetoxidans DSM 684, Ignavibacterium album JCM 16511, and Thermovibrio ammonificans HB-1. The numbers of genes in the pcc gene clusters vary, ranging from two to nine. Similar to the metal-reducing (Mtr) gene clusters of other Fe(III)-reducing bacteria, such as Shewanella spp., additional genes that encode putative c-Cyts with predicted cellular localizations at the cytoplasmic membrane, periplasm and outer membrane often associate with the pcc gene clusters. This suggests that the Pcc-associated c-Cyts may be part of the pathways for extracellular electron transfer reactions. The presence of pcc gene clusters in the microorganisms that do not reduce solid-phase Fe(III) and Mn(IV) oxides, such as D. alkaliphilus AHT2 and I. album JCM 16511, also suggests that some of the pcc gene clusters may be involved in extracellular electron transfer reactions with the substrates other than Fe(III) and Mn(IV) oxides.

  14. Edinburgh Research Explorer High-resolution gene expression profiling for simultaneous

    E-Print Network [OSTI]

    Millar, Andrew J.

    Edinburgh Research Explorer High-resolution gene expression profiling for simultaneous kinetic, UH 2008, 'High-resolution gene expression profiling for simultaneous kinetic parameter analysis date: 16. Jun. 2014 #12;METHOD High-resolution gene expression profiling for simultaneous kinetic

  15. Functional replacement of a primary metabolic pathway via multiple independent eukaryote-to-eukaryote gene transfers

    E-Print Network [OSTI]

    Nedelcu, Aurora M.

    Functional replacement of a primary metabolic pathway via multiple independent eukaryote can be added to the recipient's gene complement (gene additions) or simply replace existing endogenous counterparts (functional replacements). The recruitment of novel genes is thought to allow the recipient

  16. Multiple gene genealogies and microsatellite markers reflect relationships between morphotypes of Sphaeropsis sapinea

    E-Print Network [OSTI]

    Multiple gene genealogies and microsatellite markers reflect relationships between morphotypes between isolates representing different S. sapinea morphotypes, using multiple gene genealogies inferred from partial sequences of six protein-coding genes and six microsatellite loci. Genealogies generated

  17. Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs.

    E-Print Network [OSTI]

    Yan, M; Wen, J; Liang, M; Lu, Y; Kamata, M; Chen, IS

    2015-01-01T23:59:59.000Z

    of Gene Expression by Polymer Nanocapsule S5 Fig. Stabilityof Gene Expression by Polymer Nanocapsule Rao D, Vorhies J,of Gene Expression by Polymer Nanocapsule Delivery of DNA

  18. Toward epigenetic and gene regulation models of specific language impairment: looking for links among growth, genes, and impairments

    E-Print Network [OSTI]

    Rice, Mabel L.

    2012-11-24T23:59:59.000Z

    for candidate genes. Regulatory regions of the genes may be involved. Behavioral growth models of language development of children with SLI reveal that the onset of language is delayed, and the growth trajectories of children with SLI parallel those of younger...

  19. An adaptive radiation model for the origin of new gene functions

    E-Print Network [OSTI]

    Francino, M. Pilar

    2005-01-01T23:59:59.000Z

    An adaptive radiation model for the origin of new genePreadaptation Adaptive radiation Competition among closefor a specific niche Adaptive radiation model Adaptive gene

  20. Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria. Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria. Abstract: Microarray analysis indicated...

  1. Targeting gene expression to tumor cells with loss of wild-type p53 function

    E-Print Network [OSTI]

    Tian, Weidong

    of the carcinoembryonic antigen and -fetoprotein genes for hepatocarcinoma4,5 and of the tyrosinase gene for mel- anoma,6

  2. o,p'-DDT induces cyclooxygenase-2 gene expression in murine macrophages: Role of AP-1 and CRE promoter elements and PI3-kinase/Akt/MAPK signaling pathways

    SciTech Connect (OSTI)

    Han, Eun Hee; Kim, Ji Young; Kim, Hyung-Kyun; Hwang, Yong Pil [BK 21 Project Team, Department of Pharmacy, College of Pharmacy, Chosun University, 375 Seosuk-dong, Gwangju 501-759 (Korea, Republic of); Jeong, Hye Gwang [BK 21 Project Team, Department of Pharmacy, College of Pharmacy, Chosun University, 375 Seosuk-dong, Gwangju 501-759 (Korea, Republic of)], E-mail: hgjeong@chosun.ac.kr

    2008-12-01T23:59:59.000Z

    Dichlorodiphenyltrichloroethane (DDT) has been used as an insecticide to prevent the devastation of malaria in tropical zones. However, many reports suggest that DDT may act as an endocrine disruptor and may have possible carcinogenic effects. Cyclooxygenase-2 (COX-2) acts as a link between inflammation and carcinogenesis through its involvement in tumor promotion. In the present study, we examined the effect of o,p'-DDT on COX-2 gene expression and analyzed the molecular mechanism of its activity in murine RAW 264.7 macrophages. Exposure to o,p'-DDT markedly enhanced the production of prostaglandin E{sub 2} (PGE{sub 2}), a major COX-2 metabolite, in murine macrophages. Furthermore, o,p'-DDT dose-dependently increased the levels of COX-2 protein and mRNA. Transfection with human COX-2 promoter construct, electrophoretic mobility shift assays and DNA-affinity protein-binding assay experiments revealed that o,p'-DDT activated the activator protein 1 (AP-1) and cyclic AMP response element (CRE) sites, but not the NF-{kappa}B site. Phosphatidylinositol 3 (PI3)-kinase, its downstream signaling molecule, Akt, and mitogen-activated protein kinases (MAPK) were also significantly activated by the o,p'-DDT-induced AP-1 and CRE activation. These results demonstrate that o,p'-DDT induced COX-2 expression via AP-1 and CRE activation through the PI3-K/Akt/ERK, JNK, and p38 MAP kinase pathways. These findings provide further insight into the signal transduction pathways involved in the carcinogenic effects of o,p'-DDT.

  3. Transgenic mice expressing mutant Pinin exhibit muscular dystrophy, nebulin deficiency and elevated expression of slow-type muscle fiber genes

    SciTech Connect (OSTI)

    Wu, Hsu-Pin; Hsu, Shu-Yuan [Department of Anatomy, Chang Gung University Medical College, Taiwan (China)] [Department of Anatomy, Chang Gung University Medical College, Taiwan (China); Wu, Wen-Ai; Hu, Ji-Wei [Transgenic Mouse Core Laboratory, Chang Gung University, Taiwan (China)] [Transgenic Mouse Core Laboratory, Chang Gung University, Taiwan (China); Ouyang, Pin, E-mail: ouyang@mail.cgu.edu.tw [Department of Anatomy, Chang Gung University Medical College, Taiwan (China) [Department of Anatomy, Chang Gung University Medical College, Taiwan (China); Transgenic Mouse Core Laboratory, Chang Gung University, Taiwan (China); Molecular Medicine Research Center, Chang Gung University, Taiwan (China)

    2014-01-03T23:59:59.000Z

    Highlights: •Pnn CCD domain functions as a dominant negative mutant regulating Pnn expression and function. •Pnn CCD mutant Tg mice have a muscle wasting phenotype during development and show dystrophic histological features. •Pnn mutant muscles are susceptible to slow fiber type gene transition and NEB reduction. •The Tg mouse generated by overexpression of the Pnn CCD domain displays many characteristics resembling NEB{sup +/?} mice. -- Abstract: Pinin (Pnn) is a nuclear speckle-associated SR-like protein. The N-terminal region of the Pnn protein sequence is highly conserved from mammals to insects, but the C-terminal RS domain-containing region is absent in lower species. The N-terminal coiled-coil domain (CCD) is, therefore, of interest not only from a functional point of view, but also from an evolutionarily standpoint. To explore the biological role of the Pnn CCD in a physiological context, we generated transgenic mice overexpressing Pnn mutant in skeletal muscle. We found that overexpression of the CCD reduces endogenous Pnn expression in cultured cell lines as well as in transgenic skeletal muscle fibers. Pnn mutant mice exhibited reduced body mass and impaired muscle function during development. Mutant skeletal muscles show dystrophic histological features with muscle fibers heavily loaded with centrally located myonuclei. Expression profiling and pathway analysis identified over-representation of genes in gene categories associated with muscle contraction, specifically those related to slow type fiber. In addition nebulin (NEB) expression level is repressed in Pnn mutant skeletal muscle. We conclude that Pnn downregulation in skeletal muscle causes a muscular dystrophic phenotype associated with NEB deficiency and the CCD domain is incapable of replacing full length Pnn in terms of functional capacity.

  4. Mutational analysis of the [phi] X174 E Gene

    E-Print Network [OSTI]

    Morham, Scott Garton

    1985-01-01T23:59:59.000Z

    for the rapid isolation and sequencing of E gene mutants from the screening vector pSM120 has been developed. Two nonsense mutations have been isolated which totally inactivate the E gene product. A variety of amino acids have been inserted at these non... Hydroxylami ne mutagenesis Transformation 13 16 18 19 29 29 30 30 32 33 33 36 38 38 TABLE OF CONTENTS (continued) Selection of gene E mutations in pSM120 M13 cloning and dideoxy sequencing 3. RESULTS Characterization of deletion clones...

  5. Sorghum Ma5 and Ma6 maturity genes

    E-Print Network [OSTI]

    Brady, Jeffrey Alan

    2009-05-15T23:59:59.000Z

    the precise mechanism of function for the A. thaliana circadian clock is not fully established, at least three genes appear to be involved in the central circadian oscillator mechanism. These genes are TIMING OF CHLOROPHYLL A/B BINDING PROTEIN 1 (TOC1... the expression of CCA1 and LHY, and the gene encoding it is a target for repression by CCA1 and LHY proteins. The protein is an A. thaliana pseudo response regulator (APRR) called TIMING OF CHLOROPHYLL A/B BINDING PROTEIN 1 (TOC1), and its transcript levels...

  6. LATERAL GENE TRANSFER AND THE HISTORY OF BACTERIAL GENOMES

    SciTech Connect (OSTI)

    Howard Ochman

    2006-02-22T23:59:59.000Z

    The aims of this research were to elucidate the role and extent of lateral transfer in the differentiation of bacterial strains and species, and to assess the impact of gene transfer on the evolution of bacterial genomes. The ultimate goal of the project is to examine the dynamics of a core set of protein-coding genes (i.e., those that are distributed universally among Bacteria) by developing conserved primers that would allow their amplification and sequencing in any bacterial taxa. In addition, we adopted a bioinformatic approach to elucidate the extent of lateral gene transfer in sequenced genome.

  7. NATURE|Vol 440|9 March 2006 NEWS & VIEWS a more favourable alignment for reaction? For

    E-Print Network [OSTI]

    Nielsen, Rasmus

    to the ground. Although Sheiko et al. did not set out to capture energy from breaking car- bon­carbon bonds change? Car- bon­carbon bonds, by dint of their strength, release a lot of energy when they are broken the loss of any genes. Using this technology, Gilad and colleagues demonstrate that most genes are under

  8. Hot prospect for new gene amplifier

    SciTech Connect (OSTI)

    Not Available

    1991-11-29T23:59:59.000Z

    Molecular biologist Francis Barany is investigating one of the hottest areas in biotechnology: a gene-amplification technique called ligase chain reaction, or LCR. Already scientists have used LCR to detect the tiny mutation that causes sickle cell anemia and have adapted it to screen for a handful of other genetic diseases simultaneously - in a single test-tube. Some experts, in fact, are predicting that LCR will supplement the polymerase chain reaction (PCR), and in some cases even supplant it. LCR could revolutionize DNA diagnostics just as PCR transformed basic molecular biology following its introduction 6 years ago. With its ease of automation and ability to produce useful quantitative results, LCR could become a major player in the rapidly growing market for DNA diagnostics. LCR, like PCR, uses snippets of nucleic acid, or oligonucleotides, that anneal to a specific, complementary sequence on the target DNA to be amplified. But where PCR uses oligos that bracket the stretch to be amplified, LCR uses pairs of oligos that completely cover the target sequence.

  9. An Inclusive View of Player Modeling Adam M. Smith, Chris Lewis, Kenneth Hullett, Gillian Smith, Anne Sullivan

    E-Print Network [OSTI]

    California at Santa Cruz, University of

    An Inclusive View of Player Modeling Adam M. Smith, Chris Lewis, Kenneth Hullett, Gillian Smith, Anne Sullivan Center for Games and Playable Media, UC Santa Cruz Santa Cruz, CA 95064, USA {amsmith

  10. 350-?m side-view optical probe for imaging the murine brain in vivo from the cortex to the hypothalamus

    E-Print Network [OSTI]

    Kim, Jun Ki

    Miniature endoscopic probes offer a solution for deep brain imaging by overcoming the limited depth of intravital microscopy. We describe a small-diameter (350 ?m) graded-index optical probe with a side-view design for in ...

  11. U-Filter: A Lightweight XML View Update Checker Ling Wang, Elke A. Rundensteiner and Murali Mani

    E-Print Network [OSTI]

    Mani, Murali

    U-Filter: A Lightweight XML View Update Checker Ling Wang, Elke A. Rundensteiner and Murali Mani & Schuster Inc.A02 Prentice-Hall Inc.B01 McGraw-Hill Inc.A01 pubn

  12. Scalable multi-view stereo camera array for real world real-time image capture and three-dimensional displays

    E-Print Network [OSTI]

    Hill, Samuel L. (Samuel Lincoln), 1978-

    2004-01-01T23:59:59.000Z

    The number of three-dimensional displays available is escalating and yet the capturing devices for multiple view content are focused on either single camera precision rigs that are limited to stationary objects or the use ...

  13. Creating a methodology and tool to capture and resolve conflicts in developing software requirements: Requirement Lifecycle Modeling Views manager (RLMV)

    E-Print Network [OSTI]

    Herrera, Leeha Rae-Lyn

    2013-02-22T23:59:59.000Z

    tool that will aid in tracing requirements throughout the software development lifecycle. The tool, named Requirement Lifecycle Modeling Views (RLMV), follows the architecture, as defined in The Unified Modeling Language Users Guide, for modeling...

  14. Viewing the Web as a Virtual Database for Question Answering Boris Katz, Sue Felshin, Jimmy Lin, and Gregory Marton

    E-Print Network [OSTI]

    Lin, Jimmy

    , Jimmy Lin, and Gregory Marton MIT Computer Science and Artificial Intelligence Laboratory 200 Technology of this book chapter appears as: Boris Katz, Sue Felshin, Jimmy Lin, and Gregory Marton. Viewing the Web

  15. Transit of Venus: New Views of the Solar System and Galaxy Proceedings IAU Colloquium No. 196, 2004

    E-Print Network [OSTI]

    Transit of Venus: New Views of the Solar System and Galaxy Proceedings IAU Colloquium No. 196, 2004 to complement those, from the South Seas, by Green and Cook. Mason did not volunteer his services

  16. Transit of Venus: New Views of the Solar System and Galaxy Proceedings IAU Colloquium No. 196, 2004

    E-Print Network [OSTI]

    Transit of Venus: New Views of the Solar System and Galaxy Proceedings IAU Colloquium No. 196, 2004 and Cook. Mason did not volunteer his services on this occasion, possibly because of the rather o

  17. Patterns of satellite-viewed, subtropical, jet-stream clouds in relation to the observed wind field

    E-Print Network [OSTI]

    Vogt, Richard Joel

    1972-01-01T23:59:59.000Z

    PATTERNS OF SATELLITE-VIEWED, SUBTROPICAL, JET- STREAM CLOUDS IN RELATION TO THE OBSERVED WIND FIELD A Thesis by RICHARD JOEL VOGT Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirement... for the degree of MASTER OF SCIENCE December 1972 Major Subject: Meteorology PATTERNS OP SATELLITE-VIEWED, SUBTROPICAL, JET-STREAM CLOUDS IN RELATION TO THE OBSERVED WIND FIELD A Thesis by RICHARD JOEL VOGT Approved as to style and content by: (Chairman...

  18. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    SciTech Connect (OSTI)

    Hoen, Ben; Wiser, Ryan; Cappers, Peter; Thayer, Mark; Sethi, Gautam

    2010-04-01T23:59:59.000Z

    With an increasing number of communities considering nearby wind power developments, there is a need to empirically investigate community concerns about wind project development. One such concern is that property values may be adversely affected by wind energy facilities, and relatively little research exists on the subject. The present research investigates roughly 7,500 sales of single-family homes surrounding 24 existing U.S. wind facilities. Across four different hedonic models the results are consistent: neither the view of the wind facilities nor the distance of the home to those facilities is found to have a statistically significant effect on home sales prices.

  19. Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices

    SciTech Connect (OSTI)

    San Diego State University; Bard Center for Environmental Policy at Bard College; Hoen, Ben; Wiser, Ryan; Cappers, Peter; Thayer, Mark; Sethi, Gautam

    2011-06-23T23:59:59.000Z

    With increasing numbers of communities considering wind power developments, empirical investigations regarding related community concerns are needed. One such concern is that proximate property values may be adversely affected, yet relatively little research exists on the subject. The present research investigates roughly 7,500 sales of single-family homes surrounding 24 existing U.S. wind facilities. Across four different hedonic models, and a variety of robustness tests, the results are consistent: neither the view of the wind facilities nor the distance of the home to those facilities is found to have a statistically significant effect on sales prices, yet further research is warranted.

  20. New View on Quantum Gravity and the Origin of the Universe

    E-Print Network [OSTI]

    B. L. Hu

    2006-11-10T23:59:59.000Z

    This essay is based on a physics lecture given at the Hong Kong University of Science and Technology on May 24, 2006, Hong Kong. It has 5 sections and one page of references and suggested reading. The section titles are: 1. Origin of the Universe and Quantum Gravity 2. Two major paradigms in Physics and Two directions of Research in Cosmology 3. The Mescoscopic Structures of Spacetime and Stochastic Gravity 4. One Vein of the Hydro View: Spacetime as Condensate? 5. Implications for the Origin of the Universe and other issues.

  1. Biomass stakeholder views and concerns: Environmental groups and some trade association

    SciTech Connect (OSTI)

    Peelle, E.

    2000-01-01T23:59:59.000Z

    This exploratory study of the views and concerns of 25 environmental organizations found high interest and concern about which biomass feedstocks would be used and how these biomass materials would be converted to energy. While all favored renewable energy over fossil or nuclear energy, opinion diverged over whether energy crops, residues, or both should be the primary source of a biomass/bioenergy fuel cycle. About half of the discussants favored biomass ``in general'' as a renewable energy source, while the others were distributed about equally over five categories, from favor-with-conditions, uncertain, skeptical, opposed, to ``no organizational policy.''

  2. DOE Zero Energy Ready Home Case Study 2013: Clifton View Homes, Coupeville, WA

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels DataDepartment of Energy Your Density Isn't Your Destiny: Theof"WaveInteractionsMaterials | DepartmentEnergy Clifton View Homes

  3. View the Apps for Vehicles Phase One Submissions | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof Energy 2, 2015 -Helicopter-JapanEnergyNews14-88-LNGVideos VideosView the Apps

  4. NREL: Awards and Honors - PowerView Semi-Transparent Photovoltaic Module

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Saleshttp://www.fnal.gov/directorate/nalcal/nalcal02_07_05_files/nalcal.gifNREL NREL Refines MethodLightweight,AwardsPowerView(tm)

  5. Determination of the linkage relationships and the gene-centromere genetic distances for endopeptidase structural genes in hexaploid wheat

    E-Print Network [OSTI]

    McMillin, David Edwin

    1977-01-01T23:59:59.000Z

    . The linkage relationships for the (EP) structural gene are shown below. centromere 42. 17 ~E -Blv 13. 04 7AL ~E-Ale 6. 67 ~E-Al 3. 90 10. 67 ACKNOMLF. DGT'KNT S Sincere gratitude is extended to all members of my graduate committee for. their guidance..., or KP-BlZ and their chromosome constitut on Linkage relationships for three (EP) structural genes on 7AL Chi square test for independence of EP-Aj. y and ~E-Alx Chi square testing the hypothesis that two independently segregating genes in FI...

  6. High-throughput comparison of gene fitness among related bacteria

    E-Print Network [OSTI]

    2012-01-01T23:59:59.000Z

    licenses/by/2.0), which permits unrestricted use,Mutations in this gene permit second site mutations in hnsIt is known that rpoS mutants permit mutations in hns in

  7. antagonist gene polymorphism: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    processing, short-term memory 57 Association of Common Polymorphisms in GLUT9 Gene with Gout but Not with Coronary Artery Disease in a Large Case-Control Study CiteSeer Summary:...

  8. antigen-4 gene polymorphisms: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    processing, short-term memory 55 Association of Common Polymorphisms in GLUT9 Gene with Gout but Not with Coronary Artery Disease in a Large Case-Control Study CiteSeer Summary:...

  9. antagonist gene polymorphisms: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    processing, short-term memory 57 Association of Common Polymorphisms in GLUT9 Gene with Gout but Not with Coronary Artery Disease in a Large Case-Control Study CiteSeer Summary:...

  10. alpha gene polymorphisms: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    processing, short-term memory 60 Association of Common Polymorphisms in GLUT9 Gene with Gout but Not with Coronary Artery Disease in a Large Case-Control Study CiteSeer Summary:...

  11. ORIGINAL PAPER Identifying differentially expressed genes in human acute leukemia

    E-Print Network [OSTI]

    Gu, Xun

    ORIGINAL PAPER Identifying differentially expressed genes in human acute leukemia and mouse brain the experimental-wise false discovery rate. A human acute leukemia dataset corrected from 38 leukemia patients

  12. adipose tissue gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the expression of the UCP1 gene 293 D. Norepinephrine is an apoptosis inhibitor in brown adipocytes 294 IV. How Significant Is Brown Adipose Tissue? 295 A. Parameters of...

  13. RESEARCH Open Access Gene expression and fractionation resistance

    E-Print Network [OSTI]

    Sankoff, David

    in Paramecium, Gout et al. [10] identify a clear relationship between high WGD duplicate gene retention rates for explaining variable resistance to fractionation. The Gout et al. paper [10] is the primary inspiration

  14. PI Control of Gene Expression in Tumorous Cell Lines

    E-Print Network [OSTI]

    Mendonca, Rouella J.

    2010-01-16T23:59:59.000Z

    cancer cell line genes behave more like their Human Embryonic Kidney cell line counterparts. Two methods of intervention were introduced. The first method was the simpler on-off control intervention while the second method used a more advanced...

  15. aldosterone synthase gene: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    these genes in the CNS of male Wistar Kyoto (WKY) rats in response to systemic ACTH infusion, dexamethasone infusion, and to adrenalectomy. CYP11B1 and CYP11B2 mRNA levels were...

  16. RESEARCH Open Access In vivo gene transfer targeting in pancreatic

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    transfer of a bicistronic transgene bearing a luciferase gene and the herpes simplex virus thymidine kinase of the article © 2012 Lafitte et al.; licensee BioMed Central Ltd. This is an Open Access article distributed

  17. Hormonal interactions in progesterone regulation of gonadotropin gene expression

    E-Print Network [OSTI]

    Ghochani, Yasmin

    2009-01-01T23:59:59.000Z

    receptor (PR): PRA and PRB, both encoded by the same gene,thought to be involved in transactivational function in PRB,making PRB a specific transcriptional regulator of target

  18. Computational discovery of gene modules, regulatory networks and expression programs

    E-Print Network [OSTI]

    Gerber, Georg Kurt, 1970-

    2007-01-01T23:59:59.000Z

    High-throughput molecular data are revolutionizing biology by providing massive amounts of information about gene expression and regulation. Such information is applicable both to furthering our understanding of fundamental ...

  19. Gene Function Prediction Based on Sequence or Expression Data

    E-Print Network [OSTI]

    Horan, Kevin

    2011-01-01T23:59:59.000Z

    highly enriched in heat stress-related genes (GO:0009408, p-respond specifically to heat stress and to a much lessergene functions involved in heat stress response pathways.

  20. Intrinsic limits to gene regulation by global crosstalk

    E-Print Network [OSTI]

    Friedlander, Tamar; Guet, C?lin C; Barton, Nicholas H; Tka?ik, Gašper

    2015-01-01T23:59:59.000Z

    Gene regulation relies on the specificity of transcription factor (TF) - DNA interactions. In equilibrium, limited specificity may lead to crosstalk: a regulatory state in which a gene is either incorrectly activated due to noncognate TF-DNA interactions or remains erroneously inactive. We present a tractable biophysical model of global crosstalk, where many genes are simultaneously regulated by many TFs. We show that in the simplest regulatory scenario, a lower bound on crosstalk severity can be analytically derived solely from the number of (co)regulated genes and a suitable parameter that describes binding site similarity. Estimates show that crosstalk could present a significant challenge for organisms with low-specificity TFs, such as metazoans, unless they use appropriate regulation schemes. Strong cooperativity substantially decreases crosstalk, while joint regulation by activators and repressors, surprisingly, does not; moreover, certain microscopic details about promoter architecture emerge as global...