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We encourage you to perform a real-time search of NLEBeta
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1

One pyrimidine dimer inactivates expression of a transfected gene in xeroderma pigmentosum cells  

SciTech Connect

The authors have developed a host cell reactivation assay of DNA repair utilizing UV-treated plasmid vectors. The assay primarily reflects cellular repair of transcriptional activity of damaged DNA measured indirectly as enzyme activity of the transfected genes. They studied three plasmids (pSV2cat, 5020 base pairs; pSV2catSVgpt, 7268 base pairs; and pRSVcat, 5027 base pairs) with different sizes and promoters carrying the bacterial cat gene (CAT, chloramphenicol acetyltransferase) in a construction that permits cat expression in human cells. All human simian virus 40-transformed cells studied expressed high levels of the transfected cat gene. UV treatment of the plasmids prior to transfection resulted in differential decrease in CAT activity in different cell lines. With pSV2catSVgpt, UV inactivation of CAT expression was greater in the xeroderma pigmentosum group A and D lines than in the other human cell lines tested. The D0 of the CAT inactivation curve was 50 J X m-2 for pSV2cat and for pRSVcat in the xeroderma pigmentosum group A cells. The similarity of the D0 data in the xeroderma pigmentosum group A cells for three plasmids of different size and promoters implies they all have similar UV-inactivation target size. UV-induced pyrimidine dimer formation in the plasmids was quantified by assay of the number of UV-induced T4 endonuclease V-sensitive sites. In the most sensitive xeroderma pigmentosum cells, with all three plasmids, one UV-induced pyrimidine dimer inactivates a target of about 2 kilobases, close to the size of the putative CAT mRNA.

Protic-Sabljic, M.; Kraemer, K.H.

1985-10-01T23:59:59.000Z

2

A method to detect transfected chloramphenicol acetyltransferase gene expression in intact animals  

SciTech Connect

A rapid procedure is described for assaying chloramphenicol acetyltransferase enzyme activity in intact animals following transfection of the RSV CAT plasmid into mouse bone marrow cells by electroporation. The reconstituted mice were injected with ({sup 14}C)chloramphenicol and ethyl acetate extracts of 24-h urine samples were analyzed by TLC autoradiography for the excretion of {sup 14}C-labeled metabolites. CAT expression in vivo can be detected by the presence of acetylated {sup 14}C-labeled metabolites in the urine within 1 week after bone marrow transplantation and, under the conditions described, these metabolites can be detected for at least 3 months. CAT expression in intact mice as monitored by the urine assay correlates with the CAT expression in the hematopoietic tissues assayed in vitro. This method offers a quick mode of screening for introduced CAT gene expression in vivo without sacrificing the mice.

Narayanan, R.; Jastreboff, M.M.; Chiu, Chang Fang; Ito, Etsuro; Bertino, J.R. (Memorial Sloan-Kettering Cancer Center, New York, NY (USA))

1988-01-01T23:59:59.000Z

3

Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection  

SciTech Connect

The central theme of this dissertation is represented by the versatility of mesoporous silica nanomaterials in various applications such as catalysis and bio-applications, with main focus on biological applications of Mesoporous Silica Nanospheres (MSN). The metamorphosis that we impose to these materials from catalysis to sensing and to drug and gene delivery is detailed in this dissertation. First, we developed a synthetic method that can fine tune the amount of chemically accessible organic functional groups on the pores surface of MSN by exploiting electrostatic and size matching between the cationic alkylammonium head group of the cetyltrimethylammonium bromide (CTAB) surfactant and various anionic organoalkoxysilane precursors at the micelle-water interface in a base-catalyzed condensation reaction of silicate. Aiming nature imitation, we demonstrated the catalytic abilities of the MSNs, We utilized an ethylenediamine functional group for chelating Cu{sup 2+} as a catalytic functional group anchored inside the mesopores. Thus, a polyalkynylene-based conducting polymer (molecular wire) was synthesized within the Cu-functionalized MSNs silica catalyst. For sensing applications, we have synthesized a poly(lactic acid) coated mesoporous silica nanosphere (PLA-MSN) material that serves as a fluorescence sensor system for detection of amino-containing neurotransmitters in neutral aqueous buffer. We exploited the mesoporosity of MSNs for encapsulating pharmaceutical drugs. We examined bio-friendly capping molecules such as polyamidoamine dendrimers of generations G2 to G4, to prevent the drug leaching. Next, the drug delivery system employed MSNs loaded with Doxorubicin, an anticancer drug. The results demonstrated that these nano-Trojan horses have ability to deliver Doxorubicin to cancer cells and induce their death. Finally, to demonstrate the potential of MSN as an universal cellular transmembrane nanovehicle, we anchored positively charged dendrimers on the surface of MSN and utilize them to complex cationic DNA. The p-EGFP-CI gene-coated MSN nanocomposite was able to transfect cancer cell lines, such as human HeLa and CHO cancer cell lines. The gene carrier ability of MSNs was further proved by transfecting primary cells and cotransfecting of two different genes in cancer cell lines. In sum, MSN are versatile partners in several types of applications.

Daniela Rodica Radu

2005-12-19T23:59:59.000Z

4

Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles  

E-Print Network (OSTI)

METHODOLOGY ARTICLE Open Access Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles John A O’Brien1 and Sarah CR Lummis1,2* Abstract Background: Biolistic transfection... and nano-particles The core diameter of the particles used was 40 ± 0.8 nm or 991 ± 11 nm for the 40 nm (Alfa Aesar, MA, USA) and 1 ?m (Bio-Rad laboratories, USA) particles respec- tively. These particles have a hydrodynamic diameter of 47.2 ± 1.3 and 1060...

O'Brien, John A; Lummis, Sarah C R

2011-06-10T23:59:59.000Z

5

The transfection of rabbit articular chondrocytes is independent of ...  

Science Conference Proceedings (OSTI)

1997 Society for In Vitro Biology. 1071-2690/97 $05.00+0.00. Letter to the Editor. THE TRANSFECTION OF RABBIT ARTICULAR CHONDROCYTES IS ...

6

Most Viewed Documents - Biology and Medicine | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Biology and Medicine Most Viewed Documents - Biology and Medicine Drug Retention Times Center for Human Reliability Studies (2007) External dose-rate conversion factors for calculation of dose to the public Not Available (1988) Carbon Dioxide Sequestering Using Microalgal Systems Daniel J. Stepan; Richard E. Shockey; Thomas A. Moe; et al. (2002) Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection Daniela Rodica Radu (2005) Tolerance doses for treatment planning Lyman, J.T. (1985) Preliminary Benchmarking Efforts and MCNP Simulation Results for Homeland Security Robert Hayes (2008) Function and dynamics of aptamers: A case study on the malachite green aptamer Wang, Tianjiao (2008) Extremophiles 2004 Frank Robb (2004) Elemental mercury removal using a wet scrubber.

7

April 2013 Most Viewed Documents for Chemistry | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Chemistry April 2013 Most Viewed Documents for Chemistry Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; Chou, B.E. (1978) 123 Electrical conductivity measurements of aqueous electrolyte solutions at high temperatures and high pressures Ho, P.C.; Palmer, D.A. (1995) 102 Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 95 Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection Daniela Rodica Radu (2005) 84 Flammability characteristics of combustible gases and vapors. [249 refs] Zabetakis, M.G. (1964) 80 Molecular catalytic hydrogenation of aromatic hydrocarbons and hydrotreating of coal liquids. Yang, Shiyong; Stock, L.M. (1996)

8

April 2013 Most Viewed Documents for Biology And Medicine | OSTI, US Dept  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Biology And Medicine April 2013 Most Viewed Documents for Biology And Medicine Publications in biomedical and environmental sciences programs, 1981 Moody, J.B. (comp.) (1982) 306 Drug Retention Times Center for Human Reliability Studies (2007) 99 SURVEY OF NOISE SUPPRESSION SYSTEMS FOR ENGINE GENERATOR SETS. KRISHNA,C.R. (1999) 95 Defining the Effectiveness of UV Lamps Installed in Circulating Air Ductwork Douglas VanOsdell; Karin Foarde (2002) 84 Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection Daniela Rodica Radu (2005) 84 Advisory Committee on human radiation experiments. Supplemental Volume 2a, Sources and documentation appendices. Final report NONE (1995) 72 Dose and volume specification for reporting interstitial therapy

9

MOLECULAR THERAPY Vol. 4, No. 2, August 2001 Copyright The American Society of Gene Therapy  

E-Print Network (OSTI)

that a plasmid car- rying the full SERPINA1 on a 19-kb genomic fragment and the EBV gene EBNA1 and its family] and Liu [5] have developed a simple hydro- dynamic procedure for efficient transfection of liver cells

Ford, James

10

June 2013 Most Viewed Documents for Renewable Energy Sources...  

Office of Scientific and Technical Information (OSTI)

June 2013 Most Viewed Documents for Renewable Energy Sources Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 490 Seventh Edition Fuel Cell Handbook NETL (2004)...

11

April 2013 Most Viewed Documents for Renewable Energy Sources...  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Renewable Energy Sources Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 1252 Seventh Edition Fuel Cell Handbook NETL...

12

View-Augmented Abstractions  

Science Conference Proceedings (OSTI)

This paper introduces view-augmented abstractions, which specialize an underlying numeric domain to focus on a particular expression or set of expressions. A view-augmented abstraction adds a set of materialized views to the original domain. View augmentation ... Keywords: Numeric abstract domains, abstract-interpretation precision, view maintenance

Matt Elder; Denis Gopan; Thomas Reps

2010-10-01T23:59:59.000Z

13

User_ViewRecords  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

User Records User Records © 2011 SuccessFactors, Inc. - 1 - SuccessFactors Learning Confidential. All rights reserved. Job Aid: Viewing User Records Purpose The purpose of this job aid is to guide users through the step-by-step process of viewing their records. Each task demonstrates viewing of different records. Task A. View To-Do List Enter the web address (URL) of the user application into your browser Address field and press the Enter key. Enter your user ID in the User ID textbox. Enter your password in the Password textbox. Click Sign In. View To-Do List (filter, view) 7 Steps Task A View Completed Work 8 Steps Task B View Curriculum Status and Details 11 Steps Task C 3 3 1 2 2 1 SuccessFactors Learning v 6.4 User Job Aid Viewing User Records

14

Transactional process views  

Science Conference Proceedings (OSTI)

To enable effective interorganisational collaborations, process providers have to disclose relevant parts of their local business processes in public process views. A public process view has to be consistent with the underlying private process. Local ...

Rik Eshuis; Jochem Vonk; Paul Grefen

2011-10-01T23:59:59.000Z

15

Flow-Through Microfluidic Device for High-Efficiency Transfection of Mammalian Cells through Combined Microelectroporation and Sonoporation  

E-Print Network (OSTI)

In this study we are presenting a proof-of-concept microfluidic device that simultaneously applies the conditions required for microelectroporation and micro-sonoporation in a flow-through fashion that allows for high throughput, high efficiency transfection of mammalian cells. During the design stage, we developed a low-cost, high-resolution polymer microfabrication technique termed laser stenciling. While few other electro-sonoporation protocols have been reported, to the best of our knowledge, we are the first to incorporate microelectroporation, which has been well established in literature to be advantageous to conventional electroporation, with flow-through micro-sonoporation. When comparing transfection efficiency for our electro-sonoporation method to that of sonoporation or microelectroporation alone, we observed single batch improvements up to 20 percent and 17 percent, respectively. The average improvement in efficiency was approximately 15 percent greater than achieved with sonoporation and 10 percent greater than that of electroporation. Importantly, there was little difference in short term cell viability between the three methods (maintained at > 90 percent). The average transfection efficiency for electro-sonoporation was 81.25 percent and cell viability was 91.56 percent. Overall, we have presented a device and electro-sonoporation method that meets or outperforms the transfection efficiency and cell viability standards for HeLa cells set by other reported electroporation and sonoporation methods.

Longsine, Whitney Leigh

2011-05-01T23:59:59.000Z

16

Selective gene silencing in activated leukocytes by targeting siRNAs to the integrin lymphocyte  

E-Print Network (OSTI)

to suppress gene expression and cell proliferation only in activated lymphocytes. The siRNA-fusion proteinSelective gene silencing in activated leukocytes by targeting siRNAs to the integrin lymphocyte are resistant to lipid-based transfection in vitro and are difficult to target in vivo. We show here

Lieberman, Judy

17

Self-assembled pentablock copolymers for selective and sustained gene delivery  

SciTech Connect

The poly(diethylaminoethyl methacrylate) (PDEAEM) - Pluronic F127 - PDEAEM pentablock copolymer (PB) gene delivery vector system has been found to possess an inherent selectivity in transfecting cancer cells over non-cancer cells in vitro, without attaching any targeting ligands. In order to understand the mechanism of this selective transfection, three possible intracellular barriers to transfection were investigated in both cancer and non-cancer cells. We concluded that escape from the endocytic pathway served as the primary intracellular barrier for PB-mediated transfection. Most likely, PB vectors were entrapped and rendered non-functional in acidic lysosomes of non-cancer cells, but survived in less acidic lysosomes of cancer cells. The work highlights the importance of identifying intracellular barriers for different gene delivery systems and provides a new paradigm for designing targeting vectors based on intracellular differences between cell types, rather than through the use of targeting ligands. The PB vector was further developed to simultaneously deliver anticancer drugs and genes, which showed a synergistic effect demonstrated by significantly enhanced gene expression in vitro. Due to the thermosensitive gelation behavior, the PB vector packaging both drug and gene was also investigated for its in vitro sustained release properties by using polyethylene glycol diacrylate as a barrier gel to mimic the tumor matrix in vivo. Overall, this work resulted in the development of a gene delivery vector for sustained and selective gene delivery to tumor cells for cancer therapy.

Zhang, Bingqi

2011-05-15T23:59:59.000Z

18

Taking the long view  

NLE Websites -- All DOE Office Websites (Extended Search)

Taking the long view Taking the long view Taking the long view on environmental stewardship A newly articulated mission for environmental stewardship at the Laboratory can be summed up in a simple phrase: clean up the past, control current operations, and create a sustainable future. March 20, 2012 Los Alamos Aerial Aerial view of a canyon in Los Alamos, New Mexico. Contact Environmental Communication & Public Outreach P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505) 667-0216 Email "The future viability of the Lab hinges on demonstrating to public that we protect human health and the environment." Environmental stewardship strategy looks 50 years into the future As a way of integrating environmental protection activities into a comprehensive strategy, Kevin Smith, manager of the U.S. Department of

19

A View from Elsewhere  

E-Print Network (OSTI)

Arcadia Project Review - A View From Elsewhere Tony Hirst Department of Communication and Systems, The Open University a.j.hirst@open.ac.uk Summary This report provides an overview of activities carried out during Michaelmas Term...

Hirst, Tony

2009-01-01T23:59:59.000Z

20

Prarie View RDF  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

PRAIRIE VIEW RDF PRAIRIE VIEW RDF 2 Prairie View RDF  Located at JAAP (approx. 40 miles southwest of Chicago), 223 acres on 455 Acre Parcel  Will County Owner; Waste Management, Operator  Maximum 23-Year Life WM/Will County Methane to Energy Plant  Landfill Contract Signed w/WM in 1997 w/Gas-to- Energy Plant Clause  County Retains Gas Rights &WM Installs Gas Collection System  WM owns Methane to Energy Plant &Tax Credits 3 CONTRACT PHASE  DOE Grant Applied 6/09  County Board Approves DOE EECBG Strategy 11/09  1 Million DOE Funds to Methane to Energy Plant  Schiff Harden Hired to Negotiate Gas to Energy Contract  February 2010 County Board Authorizes Contract Execution 4 5 METHANE TO ENERGY PLANT DETAILS  Waste Management Required To

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

In vitro circadian ANP secretion by gene transferring cells encapsulated in polycaprolactone tubes: gene chronotherapy  

E-Print Network (OSTI)

A new insofar as chronobiologic therapeutic approach by atrial natriuretic peptide (ANP) for hypertension and/or congestive heart failure (CHF) is based on the release of ANP from ANP cDNA transfected Chinese Hamster Ovary (CHO) cells encapsulated in polycaprolactone (PCL) tubes. ANP secretion was maintained for at least 6 months. The encapsulated cells remained viable during culturing. Control cells without transferred ANP cDNA were negative. ANP secretion is circadian periodic, peaking around 04:18, shifted to around 07:56 by melatonin treatment. The encapsulation technique, based on principles of chronotherapy, may provide a more efficient gene therapy, applicable for eventual human implantation of gene transferred cells.

Zhengrong Wang A; Liguo Chen A; Chaomin Wan A; Yiqu A; G. Cornélissen B; F. Halberg B

2004-01-01T23:59:59.000Z

22

ParaView at NERSC  

NLE Websites -- All DOE Office Websites (Extended Search)

ParaView ParaView ParaView Introduction ParaView is an open-source, multi-platform data analysis and visualization application. ParaView users can quickly build visualizations to analyze their data using qualitative and quantitative techniques. The data exploration can be done interactively in 3D or programmatically using ParaView's batch processing capabilities. ParaView was developed to analyze extremely large datasets using distributed memory computing resources. It can be run on supercomputers to analyze datasets of terascale as well as on laptops for smaller data. Remote Visualization with ParaView ParaView is a client-server application. The ParaView client (or simply paraview) will run on your desktop while the server will run at the remote supercomputing site. The following describes the steps you will take to

23

Stereoscopic optical viewing system  

DOE Patents (OSTI)

An improved optical system which provides the operator with a stereoscopic viewing field and depth of vision, particularly suitable for use in various machines such as electron or laser beam welding and drilling machines. The system features two separate but independently controlled optical viewing assemblies from the eyepiece to a spot directly above the working surface. Each optical assembly comprises a combination of eye pieces, turning prisms, telephoto lenses for providing magnification, achromatic imaging relay lenses and final stage pentagonal turning prisms. Adjustment for variations in distance from the turning prisms to the workpiece, necessitated by varying part sizes and configurations and by the operator's visual accuity, is provided separately for each optical assembly by means of separate manual controls at the operator console or within easy reach of the operator.

Tallman, C.S.

1986-05-02T23:59:59.000Z

24

Dynamic View Management System for Query Prediction to View Materialization  

Science Conference Proceedings (OSTI)

On-Line Analytical Processing OLAP systems based on data warehouses are the main systems for managerial decision making and must have a quick response time. Several algorithms have been presented to select the proper set of data and elicit suitable structured ... Keywords: Data Warehousing, Dynamic View Materialization, OLAP, Probabilistic Reasoning Approaches, View Prediction, View Selection

Negin Daneshpour; Ahmad Abdollahzadeh Barfourosh

2011-04-01T23:59:59.000Z

25

The View from HQ  

National Nuclear Security Administration (NNSA)

A publication of the Office of Advanced Simulation & Computing, NNSA Defense Programs A publication of the Office of Advanced Simulation & Computing, NNSA Defense Programs NA-ASC-500-07-Issue 3 May 2007 The View from HQ by Dimitri Kusnezov I have been spending much of my time these days thinking about science, technology and engineering and the role of the laboratories and how that will be reflected in the Complex of the future. This is on my mind for two reasons: one is my responsibility to

26

Informative Views and Active Recognition  

E-Print Network (OSTI)

Informative Views and Active Recognition Tal Arbel, Frank P. Ferrie, and Peter Whaite TR-CIM-94-7897 Email: cim@cim.mcgill.ca #12;Informative Views and Active Recognition Tal Arbel, Frank P. Ferrie

Dudek, Gregory

27

Protective laser beam viewing device  

SciTech Connect

A protective laser beam viewing system or device including a camera selectively sensitive to laser light wavelengths and a viewing screen receiving images from the laser sensitive camera. According to a preferred embodiment of the invention, the camera is worn on the head of the user or incorporated into a goggle-type viewing display so that it is always aimed at the area of viewing interest to the user and the viewing screen is incorporated into a video display worn as goggles over the eyes of the user.

Neil, George R.; Jordan, Kevin Carl

2012-12-18T23:59:59.000Z

28

The View from HQ  

National Nuclear Security Administration (NNSA)

  NA-ASC-500-07 Issue 2 January 2007 The View from HQ Sitting in airports and planes is risky beyond the obvious dangers now in the news. Uninter- rupted time to think may lead to new ideas. Instinct instructs us that when we hear Wash- ington has some new ideas, the result must be bad. After all, ideas suggest change, which is inherently disruptive. Today the notion of predictivity is on my mind as I am leaving the V&V 2007 meeting in Los Alamos. Predictivity is on my short list of overused, ill-defined words. Washington main- tains a full lexicon of such words-a fair number of which find their way into common usage.

29

False color viewing device  

DOE Patents (OSTI)

This invention consists of a viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching, the user`s eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage.

Kronberg, J.W.

1991-05-08T23:59:59.000Z

30

False color viewing device  

DOE Patents (OSTI)

A viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching the user's eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage.

Kronberg, James W. (108 Independent Blvd., Aiken, SC 29801)

1992-01-01T23:59:59.000Z

31

False color viewing device  

DOE Patents (OSTI)

A viewing device for observing objects in near-infrared false-color comprising a pair of goggles with one or more filters in the apertures, and pads that engage the face for blocking stray light from the sides so that all light reaching the user's eyes come through the filters. The filters attenuate most visible light and pass near-infrared (having wavelengths longer than approximately 700 nm) and a small amount of blue-green and blue-violet (having wavelengths in the 500 to 520 nm and shorter than 435 nm, respectively). The goggles are useful for looking at vegetation to identify different species and for determining the health of the vegetation, and to detect some forms of camouflage. 7 figs.

Kronberg, J.W.

1992-10-20T23:59:59.000Z

32

Aging gene  

NLE Websites -- All DOE Office Websites (Extended Search)

Aging gene Name: Linda S Martinez Location: NA Country: NA Date: NA Question: Have the aging gene or genes been located on the human chromosomes, and, if yes, will removing that...

33

Informative Views and Sequential Recognition  

E-Print Network (OSTI)

Informative Views and Sequential Recognition Tal Arbel and Frank P. Ferrie TR-CIM-95-10 Nov. 1995 3 Telephone: 514 398-6319 Telex: 05 268510 FAX: 514 398-7348 Email: cim@cim.mcgill.ca #12;Informative Views

Dudek, Gregory

34

Transcriptional regulation of human retinoic acid receptor-alpha (RAR-{alpha}) by Wilms` tumour gene product  

SciTech Connect

The Wilms` tumor gene encodes a 47-49 kDa transcription factor expressed in kidney, gonads and mesothelium during embryogenesis. Inherited mutations of WT1 lead to aberrant urogenital development and Wilms` tumor, but the role of WT1 in development is not fully understood. Since the human RAR-{alpha} gene contains a potential WT1 binding site at its 5{prime} end, we studied the effect of WT1 co-transfection on expression of an RAR-{alpha} promoter/CAT reporter construct in COS cells. COS cells were plated at 5X10{sup 5} cells/dish in DMEM with 10% FBS and transfected by the Ca/PO4 method with an expression plasmid containing the full-length WT1 (-/-) cDNA under the control of the CMV promoter, plasmid containing the RAR-{alpha} promoter (-519 to +36)/CAT reporter and TK/growth hormone plasmid to control for efficiency of transfection. CAT/GH activity at 48 hours was inhibited by co-transfection with increasing amounts of WT1 (-/-); maximum inhibition = 5% of control. WT1 co-transfection did not affect expression of TKGH, nor of a CMV-CAT vector. Expression of WT1 protein in tranfected COS cells was demonstrated by Western blotting. Minimal inhibiton of RAR-{alpha}/CAT activity was seen when cells were co-transfected with vectors containing WT1 deletion mutants, alternate WT1 splicing variants, or WT1 (-/-) cDNA bearing a mutation identified in a patient with Drash syndrome. Gel shift assays indicated binding of WT1 to RAR-{alpha} cDNA but not to an RAR-{alpha} deletion mutant lacking the GCGGGGGGCG site. These observations suggest that WT1 may function to regulate RAR-{alpha} expression during normal development.

Goodyer, P.R.; Torban, E.; Dehbi, M. [and others

1994-09-01T23:59:59.000Z

35

Chisolm View | Open Energy Information  

Open Energy Info (EERE)

Chisolm View Chisolm View Jump to: navigation, search Name Chisolm View Facility Chisolm View Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner GE Energy Financial Service / Enel Green Power North America Developer TradeWind Energy Energy Purchaser Alabama Power Company Location Hunter OK Coordinates 36.59527057°, -97.54501104° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":36.59527057,"lon":-97.54501104,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

36

Evolving Views of Pectin Biosynthesis  

NLE Websites -- All DOE Office Websites (Extended Search)

BIOSYNTHESIS DURING PRIMARY AND SECONDARY WALL FORMATION Multiple lines of evidence have led to a new view of primary wall architecture and the role of pectin therein. Dick-P...

37

Risk View Software Functional Specification  

Science Conference Proceedings (OSTI)

This report defines the functional requirements for a new Risk View software product to be developed as part of the Electric Power Research Institute's (EPRI's) Operations and Maintenance Excellence (OMX) initiative. plant information sources.

2010-09-30T23:59:59.000Z

38

List View | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

List View List View Safety Data/Tools Apps Challenges Resources Blogs Let's Talk Safety You are here Data.gov » Communities » Safety List View Interactive applications that visually display large datasets provide a portal to explore data and make discoveries. Federal agencies collect information on energy production, use, natural resources, and energy infrastructure logistics and this data can be used to create calculators, interactive maps, and other applications that leverage this data. These applications provide user communities the ability to highlight the energy issues that are occurring within their communities, aid businesses plan and analyze their proposed projects, and provide a baseline for analyzing how energy resources can be most optimally and efficiently used. This page

39

Lighting for remote viewing systems  

SciTech Connect

Scenes viewed by television do not provide the same channels of information for judgment of distances as scenes viewed directly, since television eliminates or degrades several depth perception cues. However, it may be possible to improve depth perception of televised scenes by enhancing the information available through depth cues that are available from lighting. A literature survey and expert opinions were integrated to design a remote lighting arrangement which could enhance depth perception of operators performing remote handling operations. This paper describes the lighting arrangement and discusses some of its advantages and disadvantages. 10 references, 2 figures.

Draper, J.V.

1984-01-01T23:59:59.000Z

40

Lighting for remote viewing systems  

SciTech Connect

Scenes viewed by television do not provide the same channels of information for judgement of distances as scenes viewed directly, since television eliminates or degrades several depth perception cues. However, it may be possible to improve depth perception of televised scenes by enhancing the information available through depth cues that are available from lighting. A literature survey and expert opinions were integrated to design a remote lighting arrangement which could enhance depth perception of operators performing remote handling operations. This paper describes the lighting arrangement and discusses some of its advantages and disadvantages. 10 references, 2 figures.

Draper, J.V.

1984-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

View  

E-Print Network (OSTI)

NSAs have to guarantee that no private information from ...... tive to complementary cell suppression, manuscript, Energy Information Admin- istration

42

View  

E-Print Network (OSTI)

and algorithms in this area, as well as several application areas where ...... solver reached assignment for proof timeout) found of SAT. Gap. 269. 51. 2. 20. 664.

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1.2 Notation and organization of the paper ... This paper is organized as follows. ..... i is the i-th eigenvalue of C. Then, letting ˜R := QT RQ and ˜H := QT HQ, we.

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Science Conference Proceedings (OSTI)

Aug 6, 2010 ... (doi:10.1007/s00134-010-1979-1) contains supplementary material, which is .... The visual inspection of the distribution of the EQ-5D scores ...

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Science Conference Proceedings (OSTI)

Feb 16, 1998 ... temperatures and quenched by injecting helium gas. All processes ..... retrofitted with “mushroom” shaped TiB2-G cathode elements and op- erated for ..... same time improving the formability of alloys and reducing the natural aging rate. .... of many magnesium die castings in production vehicles. In order for ...

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Oct 30, 2002 ... Turing machine model, our analysis yields an O(n. 3.5 .... analysis of Vavasis and Ye's algorithm is based on the notion of crossover event,.

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May 12, 2005 ... Our algorithm is a result of the combination of stochastic ap- proximation ideas ... 2EDF R&D ... 3EDF R&D ..... Let us state the main result of this section: ..... IEEE Transactions on Systems, Science and Cybernetics, 5:307. 14.

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1France Télécom R&D, 38-40 rue du gl Leclerc, 92130 Issy-les-Moulineaux, France ... The results obtained outperform many methods based on earlier literature. ...... Linear programming under uncertainty, Management Science, 1 (

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Sep 22, 2006 ... minimizing the cost of the thermal power production if the hydro-power plant cannot supply the demand completely. Dynamic programming is a ...

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Jul 13, 2012 ... Current emphasis: visualization, data mining and applications in oil and ..... Internships. Agreement Enhancements. • Discount on indirect costs.

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Jan 27, 2011 ... Cost Horizons and Certainty Equivalents: An Approach to Stochastic Programming of Heating Oil. Management Science, 4(3):253–. 263, 1958.

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Optimization Technical Report. ISyE Department, University of Wisconsin- Madison. Oktay Günlük1 · Jeff Linderoth2. Perspective Reformulations of Mixed Integer.

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Science Conference Proceedings (OSTI)

Red Herring Dec 2001. “ Nanotechnology is the understanding and control of matter at dimensions of roughly 1 to. 100 nanometers, where unique phenomena .

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We tested these formu- ... and finding radiation treatment plans in the presence of organ motion. ...... We report the number instances for which the heuristic.

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Jul 18, 2008 ... Let us define the energy product and corresponding energy norm with respect to an ...... 3.2 Shape optimization in 3D for EIT and DC resistivity.

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2003. The Solver Manuals. GAMS Development Corporation, Washington, DC, USA. Gilbert, J. 2007a. Organization of the Modulopt collection of optimization ...

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erwin@gams.com. GAMS Development Corp. Washington DC. 19th June 2001. Abstract. The `Progressive Party Problem' [9] has long been considered a prob-.

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Oct 6, 2004... and Systems Engineering, Georgia Institute of Technology, Atlanta, .... The methods described in this paper reduce the energy function (1) at ...

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digital communication networks. .... bound. IEEE Transactions on Communications, 52:632–642, 2004. ... uscript, Georgia Institute of Technology, 2007.

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It models optimal placement of communications relay nodes in the pres- ence of obstacles. This problem ...... tion Technology (CENIIT). The authors would like to

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61

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Abstract—We consider the classical problem of estimating ... (a) to provide an estimate fn of f such that the first 2n+1 .... course at a higher computational cost.

62

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an astutely selected small working set does not significantly increase the total number of iterations required to solve the problem, and sometimes even reduces it ...

63

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Introduction. Consider the linear programming problem in the standard form [5, 14] minimize ... row index, say i, has to be determined such that ?j = min {¯bl/¯al,j  ...

64

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As is known to us, Cognition process is the instinct learning ability of human being, ..... What's more, to alleviate the undesirable effects of estimation error in the ...

65

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M? denotes the solar mass. which mimic the corresponding natural processes, ..... Gravitational radiation. In S. W. Hawking and W. Israel, editors,. 300 Years of ...

66

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Science Conference Proceedings (OSTI)

Energy Conversion – Photovoltaic, Concentrating Solar Power, and Thermoelectric ... Green Technologies for Materials Manufacturing and Processing IV.

67

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Science Conference Proceedings (OSTI)

Feb 15, 2011 ... cost for low power plant ... Fitness for small electricity grids, reduced .... Need a factory to make the price attractive, need an attractive price to.

68

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tioning, clearly identify the utility function that characterizes their attitude toward risk. The use of expected ...... with largest fixed interest rate. Alternatively, our ...

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to the performance of the exact method of [1]. They are very comparable to those of [8], although have a higher rate of increase with dimension d and/or number ...

70

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breakpoint at ?, and given by the two lines L1 and L2. Clearly, if ?L(v) ..... overhead which would be, for example, incurred at every node of a branch- and-

71

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availability-manufacturing, processing, fabrication joining, uniformity, balance of properties (what properties are important limiting), qualification, codes, safety ...

72

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Aug 30, 2004 ... (where s?1 denotes the vector of reciprocals of the components of s), so that necessarily x = µs?1 > 0, ... be written in the more symmetrical form.

73

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The colon cancer dataset contains 22 normal and 40 colon cancer tissues ..... P. Wolfe, An algorithm for quadratic programming, Naval Research Logistics.

74

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Oct 14, 2009 ... where A?(y) = ?i yiA(i) maps Rm to Sn, C is the cone of copositive ...... [14] Lovász, L. (1979), “On the Shannon capacity of a graph,” IEEE ...

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May 22, 2003 ... although the corresponding proximity function does not have a ..... Suppose that the statement of the lemma does not hold, i.e., ...... 397–413.

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Naive exploration of this neighborhood is computationally ..... Table 2: Problem Sizes for Production Planning Test Instances. In both Table 1 and Table 2, the ...

77

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Note to Practitioners—In practice, production scheduling is a crucial task at ..... the search when the exploration is close to the root, and thus makes cutoff less ...

78

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Mar 1, 2007 ... Some of the most successful methods are: Tabu search in [Gho03], a combination of Tabu ... equality constraints are dualized as before, but they also enter the ... term it is possible to avoid fast increase of the Lagrangian dual ...

79

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of image reconstruction from projections in [26], significant and valuable ac- ... ods, which makes them successful in real-world applications, is computa-. 4 ...

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[23]T. S. Motzkin. The arithmetic-geometric inequality. In Inequalities ( Proceedings of Symposium Wright. Patterson Air Force Base, Ohio, 1965), pages 205–224.

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81

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each component is non-zero precisely once in every cycle of n iterations. Thus, ... and these may not inherit the overall rate of the combined pair. Similarly, in.

82

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Sep 18, 2009 ... telecommunication industry [49] which can be reformulated as a two-stage ...... problem under equilibrium constraints in electricity spot market ...

83

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Science Conference Proceedings (OSTI)

Jun 6, 2012 ... fossil fuels and chart a new course on energy in this country, we are condemning future generations to global catastrophe.” Senator Obama ...

84

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Oct 19, 2010 ... In aircraft routing, maintenance events replenish aircrafts' ability to fly. In .... with several different label data structure and treatment strategies.

85

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of an extension of the Fundamental Theorem of Linear Programming, and proved , in ..... with ?v for v = [0,..., 0, 1] ? Rn (it does not matter that Nowak drops the last row and ...... Handbook of Combinatorial Optimization (supp. Vol. A), 1–74.

86

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and Boyd (1996), Todd (2001), the SDP handbook edited by Wolkowicz et al. ..... cut polytope CHULL(G) does not admit a polynomial time separation oracle, but ...... problem. In cutting plane algorithms it is of fundamental importance that re-.

87

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s k C bakc D s k C bas a.s k/c s k C bas .s k/c D s k C as .s k/ D as; the claim follows. Lemma A.2 (Chernoff bounds, see Mitzenmacher and Upfal [17]). Let X1;:

88

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In order to increase the search capability of MBH, a population framework has been proposed ... The key idea is to avoid new individuals to enter the population if someone similar (in a ..... We measure the efficiency in terms of number of (two-.

89

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These plugins have two essential callbacks that are called .... assigned to opened facilities so that the total sum of connection distances is minimized and the ...

90

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Feb 28, 2012 ... assumed to be essential and the only decision criterion is the travel distance. The portion ... On the other side, customers choose the facilities to.

91

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Rep., Argonne National Laboratory, Argonne,. Illinois, USA, 1992. [2] A. Borzi and K. Kunisch, A globalization strategy for the multigrid solution of elliptic optimal ...

92

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one additional order of magnitude) by finding the best axis-parallel ellipsoid, which is however too expensive to be done (outside the root node) within our ...

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[8] J. Eckstein and M. Fukushima, Some reformulation and applications of the alternating ... [10] M. Fukushima, Application of the alternating direction method of

94

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Feb 5, 2002 ... environment (“profile”) of an amino acid in terms of its contact shells. ..... that in order to use the MaxF heuristic we need to be able to load all.

95

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However, numerical simulations allow us to conjecture ... property of the optimal weights a?0,...,a?n and also an illustrative example of ... Let us define the weighted sum of exponential functions for a ? RN+1 and h ...... be two azimuth angles. ... [2] A. Labeyrie, Resolved imaging of extra-solar planets with future 10-

96

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trajectories, dissipative dynamical systems, Lyapunov analysis, weak .... the link with some classical results concerning semi-groups of contractions generated.

97

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borhood and a curve is built by linking all the points. The coordinates of ..... Learning algorithms could also be used to automatically guide the search through ...

98

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E-Print Network (OSTI)

by learning mathematical principles, and subsequent implementation of mathematical ... area under a curve as a sum of areas of rectangles as the width of each ...

99

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does not apply here because the objective function of LFMP may be unbounded ...... Handbook of Global Optimization, Kluwer Academic Publishers, 495-608.

100

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E-Print Network (OSTI)

Department of Applied Mathematics, Beijing Jiaotong University, Beijing ... (SDP), plays a fundamental role in mathematical programming, see, e.g., [21, 2, 7]. ...... [ 21] H. Wolkowicz, R. Saigal, L. Vandenberghe: Handbook of Semidefinite ...

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Feb 22, 2002 ... This problem is of fundamental importance in mathematics and physical sciences ... ?Department of Mathematics and Statistics, University of Guelph, Ontario N1G 2W1, Canada. Email: ...... Handbook of Semidefinite Program-.

102

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G as a road system, T as petrol stations and a driver who wants to go from u to v. Then B(u, v) is ..... [9] (gas distribution) provide instances where almost all ...

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E-Print Network (OSTI)

Jul 3, 2007 ... some cases, give conjectures in a fully automated way). Considering that ..... The input of the program is the definition of a given problem written in a text file. .... For instance, one can get statistics ... To determine S, GraPHedron does not need to access the graphs. ..... on Graph Theory (Novi Sad, 1983), pp.

104

Census and viewing of organisms  

NLE Websites -- All DOE Office Websites (Extended Search)

Census and viewing of organisms Census and viewing of organisms Name: m hariaczyi Status: N/A Age: N/A Location: N/A Country: N/A Date: Around 1993 Question: How many organisms exist in the world today? What is the most powerful microscope that could be used for viewing organism? Replies: The most powerful microscope is called an electron microscope, which can be used for viewing entire organisms, although few organisms are small enough to see all of them at high magnifications allowed by this microscope. So most often its used to look at fixed sections of organisms. Since the electron microscope only works in a vacuum, with no air, you cannot look at live organisms. To do that, probably the most powerful microscope is called a Nomarski, or in technical terms, a "differential interference contrast" microscope. This is a modification of a normal light microscope that allows better contrast in living tissue. It is not any more powerful than a light microscope, and is much less powerful than an electron microscope, but it allows you to see living things much better.

105

Bayesian networks: A teacher's view  

Science Conference Proceedings (OSTI)

Teachers viewing Bayesian network-based proficiency estimates from a classroom full of students face a different problem from a tutor looking at one student at a time. Fortunately, individual proficiency estimates can be aggregated into classroom and ... Keywords: Aggregation, Bayesian networks, Computer graphics, Probabilities

Russell G. Almond; Valerie J. Shute; Jody S. Underwood; Juan-Diego Zapata-Rivera

2009-03-01T23:59:59.000Z

106

On the computation of relational view complements  

Science Conference Proceedings (OSTI)

Views as a means to describe parts of a given data collection play an important role in many database applications. In dynamic environments where data is updated, not only information provided by views, but also information provided by data sources yet ... Keywords: Relational algebra, data warehouses, minimal complements, self-maintainability, view complements, views

Jens Lechtenbörger; Gottfried Vossen

2003-06-01T23:59:59.000Z

107

Trichoderma genes  

DOE Patents (OSTI)

Described herein are novel gene sequences isolated from Trichoderma reesei. Two genes encoding proteins comprising a cellulose binding domain, one encoding an arabionfuranosidase and one encoding an acetylxylanesterase are described. The sequences, CIP1 and CIP2, contain a cellulose binding domain. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.

Foreman, Pamela (Los Altos, CA); Goedegebuur, Frits (Vlaardingen, NL); Van Solingen, Pieter (Naaldwijk, NL); Ward, Michael (San Francisco, CA)

2012-06-19T23:59:59.000Z

108

Views of the solar system  

SciTech Connect

Views of the Solar System has been created as an educational tour of the solar system. It contains images and information about the Sun, planets, moons, asteroids and comets found within the solar system. The image processing for many of the images was done by the author. This tour uses hypertext to allow space travel by simply clicking on a desired planet. This causes information and images about the planet to appear on screen. While on a planet page, hyperlinks travel to pages about the moons and other relevant available resources. Unusual terms are linked to and defined in the Glossary page. Statistical information of the planets and satellites can be browsed through lists sorted by name, radius and distance. History of Space Exploration contains information about rocket history, early astronauts, space missions, spacecraft and detailed chronology tables of space exploration. The Table of Contents page has links to all of the various pages within Views Of the Solar System.

Hamilton, C.

1995-02-01T23:59:59.000Z

109

Gene silencing in cancer cells using siRNA : genetic and functional studies  

E-Print Network (OSTI)

Sequence-specific small interfering RNA (siRNA) duplexes can be used for gene silencing in mammalian cells and as mechanistic probes for determining gene function. Transfection of siRNA for specificity protein 1 (Sp1) in MCF-7 or ZR-75 cells decreased Sp1 protein in nuclear extracts, and immunohistochemical analysis showed that Sp1 protein in transfected MCF-7 cells was barely detectable. Decreased Sp1 protein in MCF-7 was accompanied by a decrease in basal and estrogen-induced transactivation and cell cycle progression. These results clearly demonstrate the key role of Sp1 protein in regulating growth and gene expression of breast cancer cells. The aryl hydrocarbon (AhR) is a ligand-activated nuclear transcription factor. siRNA for the AhR decreased TCDD-induced CYP1A1 protein, CYP1A1dependent activity, and luciferase activity in cells transfected with an Ah-responsive construct. 17?-Estradiol (E2) induces proliferation of MCF-7 cells, and this response is inhibited in cells cotreated with E2 plus TCDD. The effects of TCDD on E2-induced cell cycle progression were partially blocked in MCF-7 cells transfected with siRNA for AhR. The decrease in AhR protein in MCF-7 cells was also accompanied by increased G0/G1 ? S phase progression. Surprisingly, TCDD alone induced G0/G1 ? S phase progression and exhibited estrogenic activity in MCF-7 cells transfected with siRNA for the AhR. In contrast, degradation of the AhR in HepG2 liver cancer cells resulted in decreased G0/G1 ? S phase progression, and this was accompanied by decreased expression of cyclin D1, cyclin E, cdk2 and cdk4. In the absence of ligand, the AhR exhibits growth inhibitory (MCF-7) and growth promoting (HepG2) activity that is cell context-dependent. Sp family proteins play a complex role in regulation of pancreatic cancer cells growth and expression of genes required for growth, angiogenesis and apoptosis. Sp1, Sp3 and Sp4 cooperatively activate VEGF promoter constructs in these cells; however, only Sp3 regulates cell proliferation. siRNA for Sp3 inhibits phosphorylation of retinoblastoma protein, blocks G0/G1 ? S phase progression of Panc-1 cells, and upregulates p27 protein/promoter activity. Thus, Sp3 plays a critical role in angiogenesis (VEGF upregulation) and the proliferation of Panc-1 cells by a novel mechanism of Sp3-dependent suppression of the cyclin-dependent kinase inhibitor p27.

Abdel Rahim, Ma'en Ahmad

2004-05-01T23:59:59.000Z

110

The human estrogen receptor can regulate exogenous but not endogenous vitellogenin gene promoters in a Xenopus cell line  

SciTech Connect

Transfection of a human estrogen receptor cDNA expression vector (HEO) into cultured Xenopus kidney cells confers estrogen responsiveness to the recipient cells as demonstrated by the hormone dependent expression of co-transfected Xenopus vitellogenin-CAT chimeric genes. The estrogen stimulation of these vit-CAT genes is dependent upon the presence of the vitellogenin estrogen responsive element (ERE) in their 5{prime} flanking region. Thus, functional human estrogen receptor (hER) can be synthesized in heterologous lower vertebrate cells and can act as a trans-acting regulatory factor that is necessary, together with estradiol, for the induction of the vit-CAT constructs in these cells. In addition, vitellogenin minigenes co-transfected with the HEO expression vector also respond to hormonal stimulation. Their induction is not higher than that of the vit-CAT chimeric genes. It suggests that in the Xenopus kidney cell line B 3.2, the structural parts of the vitellogenin minigenes do not play a role in the induction process. Furthermore, no stabilizing effect of estrogen on vitellogenin mRNA is observed in these cells.

Seiler-Tuyns, A.; Merillat, A.M.; Haefliger, D.N.; Wahli, W. (Universite de Lausanne (Switzerland))

1988-09-12T23:59:59.000Z

111

September 2013 Most Viewed Documents for Renewable Energy Sources | OSTI,  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for Renewable Energy Sources September 2013 Most Viewed Documents for Renewable Energy Sources Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 362 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 103 Chapter 17. Engineering cost analysis Higbee, Charles V. (1998) 79 Advanced Electric Submersible Pump Design Tool for Geothermal Applications Xuele Qi; Norman Turnquist; Farshad Ghasripoor (2012) 79 A study of lead-acid battery efficiency near top-of-charge and the impact on PV system design Stevens, J.W.; Corey, G.P. (1996) 76 PROPERTIES AND PERFORMANCE OF CEMENT-BASED GROUTS FOR GEOTHERMAL HEAT PUMP APPLICATIONS. ALLAN,M.L. (1999) 70 Solar radiation data manual for flat-plate and concentrating collectors Dunlap, M.A. [ed.]; Marion, W.; Wilcox, S. (null)

112

Logical queries over views: Decidability and expressiveness  

Science Conference Proceedings (OSTI)

We study the problem of deciding the satisfiability of first-order logic queries over views, with our aim to delimit the boundary between the decidable and the undecidable fragments of this language. Views currently occupy a central place in database ... Keywords: Löwenheim class, Satisfiability, conjunctive query, containment, database query, database view, decidability, first-order logic, monadic logic, ontology reasoning, unary logic, unary view

James Bailey; Guozhu Dong; Anthony WIDJAJA To

2010-01-01T23:59:59.000Z

113

Gene Frequency  

NLE Websites -- All DOE Office Websites (Extended Search)

Gene Frequency Gene Frequency Name: donna Location: N/A Country: N/A Date: N/A Question: If six fingers is a dominant human trait why do we have only five? Replies: This is simple. There are just not many genes in the human population for six fingers. Steve Sample Look in any high school biology book for what is known as Hardy-Weinberg equilibrium. These two scientists (separately) said that gene frequencies do not change much unless something in the environment selects them over other genes. In other words, unless 6 fingers somehow becomes an advantage, and five-fingered people have less of an advantage, the frequency of six fingered people in the population will not necessarily increase. This is the same reason that recessive traits don't disappear from the population. Also, six fingers is not considered attractive and they may not get as many mates. Also, more people are born with six fingers than you might imagine but just have them amputated shortly after birth.

114

A Regulator's View of Cogeneration  

E-Print Network (OSTI)

The Pennsylvania Public Utility Commission regulates essentially all types of public utilities and has the authority to investigate issues of public interest. To establish a point of reference, Pennsylvania's utilities contribute about 5 percent of the total national electric generation. In view of the energy requirements of Pennsylvania's industry and the impact of increasing energy costs on employment the Commission directed its technical staff to investigate the potential for industrial cogeneration and a pricing formula consistent with the electric utilities' costs. The Commission's technical staff has completed proposed regulations to implement the provisions of the Public Utility Regulatory Policies Act (PURPA) Section 210 concerning small power producers. The regulations incorporate suggestions from both potential producers and utilities. Staff has devised a strategy for utility purchases of energy and capacity which should be of interest to regulators in other jurisdictions, encourage potential cogenerators and satisfy utilities.

Shanaman, S. M.

1982-01-01T23:59:59.000Z

115

TotalView Parallel Debugger at NERSC  

NLE Websites -- All DOE Office Websites (Extended Search)

Totalview Totalview Totalview Description TotalView from Rogue Wave Software is a parallel debugging tool that can be run with up to 512 processors. It provides both X Windows-based Graphical User Interface (GUI) and command line interface (CLI) environments for debugging. The performance of the GUI can be greatly improved if used in conjunction with free NX software. The TotalView documentation web page is a good resource for learning more about some of the advanced TotalView features. Accessing Totalview at NERSC To use TotalView at NERSC, first load the TotalView modulefile to set the correct environment settings with the following command: % module load totalview Compiling Code to Run with TotalView In order to use TotalView, code must be compiled with the -g option. We

116

Adaptation of user views to business requirements: towards adaptive views models  

Science Conference Proceedings (OSTI)

This article describes an approach to model user views in service-oriented groupware systems in order to support the adaptation of views in collaborative projects environments. The issue is to adapt the visualization to the users' business requirements ... Keywords: AEC (architecture engineering construction), CSCW, information visualization, model driven engineering, user view, user view model

Conrad Boton; Sylvain Kubicki; Gilles Halin

2010-09-01T23:59:59.000Z

117

Low doses of neutrons induce changes in gene expression  

SciTech Connect

Studies were designed to identify genes induced following low-dose neutron but not following {gamma}-ray exposure in fibroblasts. Our past work had shown differences in the expression of {beta}-protein kinase C and c-fos genes, both being induced following {gamma}-ray but not neutron exposure. We have identified two genes that are induced following neutron, but not {gamma}-ray, exposure: Rp-8 (a gene induced by apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency (HIV). Rp-8 mRNA induction was demonstrated in Syrian hamster embryo fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.5 cGy/min) and at high dose rate (12 cGy/min). The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measures of CAT activity and CAT transcripts following irradiation demonstrated an unresponsiveness to {gamma} rays over a broad range of doses. Twofold induction of the HIV-LTR was detected following neutron exposure (48 cGy) administered at low (0.5 cGy/min) but not high (12 cGy/min) dose rates. Ultraviolet-mediated HIV-LTR induction was inhibited by low-dose-rate neutron exposure.

Woloschak, G.E.; Chang-Liu, C.M. [Argonne National Lab., IL (United States); Panozzo, J.; Libertin, C.R. [Loyola Univ., Maywood, IL (United States)

1993-06-01T23:59:59.000Z

118

Low doses of neutrons induce changes in gene expression  

SciTech Connect

Studies were designed to identify genes induced following low-dose neutron but not following [gamma]-ray exposure in fibroblasts. Our past work had shown differences in the expression of [beta]-protein kinase C and c-fos genes, both being induced following [gamma]-ray but not neutron exposure. We have identified two genes that are induced following neutron, but not [gamma]-ray, exposure: Rp-8 (a gene induced by apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency (HIV). Rp-8 mRNA induction was demonstrated in Syrian hamster embryo fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.5 cGy/min) and at high dose rate (12 cGy/min). The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measures of CAT activity and CAT transcripts following irradiation demonstrated an unresponsiveness to [gamma] rays over a broad range of doses. Twofold induction of the HIV-LTR was detected following neutron exposure (48 cGy) administered at low (0.5 cGy/min) but not high (12 cGy/min) dose rates. Ultraviolet-mediated HIV-LTR induction was inhibited by low-dose-rate neutron exposure.

Woloschak, G.E.; Chang-Liu, C.M. (Argonne National Lab., IL (United States)); Panozzo, J.; Libertin, C.R. (Loyola Univ., Maywood, IL (United States))

1993-01-01T23:59:59.000Z

119

Answering XML queries using materialized views revisited  

Science Conference Proceedings (OSTI)

Answering queries using views is a well-established technique in databases. In this context, two outstanding problems can be formulated. The first one consists in deciding whether a query can be answered exclusively using one or multiple materialized ... Keywords: XML, materialized views, xpath query evaluation

Xiaoying Wu; Dimitri Theodoratos; Wendy Hui Wang

2009-11-01T23:59:59.000Z

120

Designing presentations for on-demand viewing  

Science Conference Proceedings (OSTI)

Increasingly often, presentations are given before a live audience, while simultaneously being viewed remotely and recorded for subsequent viewing on-demand over the Web. How should video presentations be designed for web access? How is video accessed ... Keywords: digital library, streaming media, video on-demand

Liwei He; Jonathan Grudin; Anoop Gupta

2000-12-01T23:59:59.000Z

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121

Query and update through XML views  

Science Conference Proceedings (OSTI)

XML has become a standard medium for data exchange, and XML views are frequently used as an interface to relational database and XML data. There have been a considerable number of studies on building and querying XML views, while updating related topics ...

Gao Cong

2007-10-01T23:59:59.000Z

122

User_Sup_ViewEmpMatrix  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

an Employee Matrix (Supervisor) an Employee Matrix (Supervisor) © 2011 SuccessFactors, Inc. - 1 - SuccessFactors Learning Confidential. All rights reserved. Job Aid: Viewing an Employee Matrix (Supervisor) Purpose The purpose of this job aid is to guide supervisor users through the step-by-step process of viewing an employee matrix within SuccessFactors Learning. Task A. View an Employee Matrix From the Home page, click the My Employees tab. Click the Employee Matrix supervisor link. Click the Change Measures expand arrow ( ) to select criteria for comparison. View an Employee Matrix 8 Steps Task A 3 3 1 1 2 2 SuccessFactors Learning v 6.4 User Job Aid Viewing an Employee Matrix (Supervisor) © 2011 SuccessFactors, Inc. - 2 - SuccessFactors Learning Select a measure for the

123

Mountain View Grand | Open Energy Information  

Open Energy Info (EERE)

Grand Grand Jump to: navigation, search Name Mountain View Grand Facility Mountain View Grand Sector Wind energy Facility Type Small Scale Wind Facility Status In Service Owner Mountain View Grand Developer Sustainable Energy Developments Energy Purchaser Mountain View Grand Location Mountain View Grand Resort & Spa NH Coordinates 44.397987°, -71.590306° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":44.397987,"lon":-71.590306,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

124

Search and View a Service Request Search and View a Service Request,vlr  

E-Print Network (OSTI)

FAMIS Search and View a Service Request Search and View a Service Request,vlr 10/6/2012 © 2012 Northwestern University 1 Search and View a Service Request This training guide will show you how to search completion of this guide you will be able to: · Sign in to FAMIS Self Service · Search for service requests

Shull, Kenneth R.

125

Activation of the human beta interferon gene by the adenovirus type 12 E1B gene  

SciTech Connect

The transcription of endogenous beta interferon mRNA was activated in human embryo kidney (HEK) cells infected with adenovirus 12 (Ad12) but was activated only inefficiently or not at all in HEK cells infected with Ad5 and rc-1 (Ad5 dl312 containing the Ad12 E1A region). The analysis with Ad12 mutants showed that Ad12 E1B products, especially the 19K protein, were important for the expression of the endogenous beta interferon gene and Ad12 E1A products were not involved in the expression. The expression of exogeneously transfected pIFN-CAT (a hybrid plasmid having the human beta interferon promoter fused with the CAT gene) was activated in HEK and chicken embryo fibroblast (CEF) cells infected with either Ad12 or Ad5. The analysis of cotransfection of CEF cells with pIFN-CAT and plasmids containing fragments of Ad12 or Ad5 DNA showed that Ad12 or Ad5 E1B (possibly the 19K protein) was and E1A was not involved in the expression of the exogenous pIFN-CAT.

Shiroki, K.; Toth, M.

1988-01-01T23:59:59.000Z

126

Lake View Geothermal Facility | Open Energy Information  

Open Energy Info (EERE)

Page Page Edit with form History Facebook icon Twitter icon » Lake View Geothermal Facility Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Lake View Geothermal Facility General Information Name Lake View Geothermal Facility Facility Lake View Sector Geothermal energy Location Information Location The Geysers, California Coordinates 38.823527148671°, -122.78173327446° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":38.823527148671,"lon":-122.78173327446,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

127

Berkeley Lab View -- March 28, 2008  

NLE Websites -- All DOE Office Websites (Extended Search)

March 28th, 2008 Search the View Archive March 28th, 2008 Search the View Archive State of the Lab: New Initiatives, Construction Daniel Chemla (1940-2008): A Remembrance of His Career The View is Going Green DOE Excellence Award to Foundry Project Team Berkeley Lab View Here Comes BELLA: The BErkeley Lab Laser Acceleration Project Berkeley Lab Science Roundup State of the Lab: New Initiatives, Construction By Lynn Yarris image Photo by Roy Kaltschmidt, CSO Free electron lasers with attosecond capabilities, a high-energy electron accelerator less than a meter in length, the arrival of NERSC-6 and the departure of GELCO-4 were some of the highlights of Berkeley Lab Director Steve Chu's State-of-the-Lab address, which he delivered at the Building 50 Auditorium during the noon hour on March 10, with simulcast to the

128

A common-view disciplined oscillator  

SciTech Connect

This paper describes a common-view disciplined oscillator (CVDO) that locks to a reference time scale through the use of common-view global positioning system (GPS) satellite measurements. The CVDO employs a proportional-integral-derivative controller that obtains near real-time common-view GPS measurements from the internet and provides steering corrections to a local oscillator. A CVDO can be locked to any time scale that makes real-time common-view data available and can serve as a high-accuracy, self-calibrating frequency and time standard. Measurement results are presented where a CVDO is locked to UTC(NIST), the coordinated universal time scale maintained at the National Institute of Standards and Technology in Boulder, Colorado.

Lombardi, Michael A. [Time and Frequency Division, National Institute of Standards and Technology (NIST), Boulder, Colorado 80305 (United States); Dahlen, Aaron P. [Loran Support Unit, United States Coast Guard (USCG), Wildwood, New Jersey 08260 (United States)

2010-05-15T23:59:59.000Z

129

Incorporating video into Google Mobile Street View  

E-Print Network (OSTI)

Mobile Street View is a compelling application but suffers from significant latency problems, especially in limited bandwidth circumstances. Currently, the application uses static images to display street level information. ...

Wright, Christina (Christina E.)

2010-01-01T23:59:59.000Z

130

A view-sequential 3D display  

E-Print Network (OSTI)

This thesis outlines the various techniques for creating electronic 3D displays and analyzes their commercial potential. The thesis argues for the use of view-sequential techniques in the design of 3D displays based on ...

Cossairt, Oliver S. (Oliver Strider), 1978-

2003-01-01T23:59:59.000Z

131

The new option view of investment  

E-Print Network (OSTI)

This paper provides a simple introduction to the new option view of investment. We explain the shortcomings of the orthodox theory, and then outline the basic ideas behind the option framework. Several industry examples ...

Dixit, Avinash K.

1995-01-01T23:59:59.000Z

132

Identification of cis-acting sequences responsible for phorbol ester induction of human serum amyloid A gene expression via a nuclear factor kB-like transcription factor  

Science Conference Proceedings (OSTI)

The authors have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). They found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, they placed a 265-base-pair 5' SAA fragment upstream of the reporter chloramphenicol acetyltransferase (CAT) gene and transfected this construct into HeLa cells. PMA treatment of these transient transfectants resulted in increased CAT expression. Nuclear proteins from PMA-treated HeLa cells bound to this DNA fragment, and methylation interference analysis showed that the binding was specific to the sequence GGGACTTTCC (between -82 and -91), a sequence previously described by others as the binding site for the nuclear factor NF/kappa/B. In a cotransfection competition experiment, they could abolish PMA-induced CAT activity by using cloned human immunodeficiency virus long-terminal-repeat DNA containing the NF/kappa/B-binding sequence. The same long-terminal-repeat DNA containing mutant NF/kappa/B-binding sequences did not affect CAT expression, which suggested that binding by an NF/kappa/B-like factor is required for increased SAA transcription.

Edbrooke, M.R.; Cheshire, J.K.; Woo, P.; Burt, B.W.

1989-05-01T23:59:59.000Z

133

Characterization of the mouse thrombospondin 2 gene  

Science Conference Proceedings (OSTI)

The authors have characterized the exon/intron organization, complete 3[prime] untranslated region (3[prime]-UTR), and approximately 2.5 kb of the promoter/5[prime] flanking region of the mouse thrombospondin 2 (TSP2) gene. The sizes of exons and the pattern of interruption of the reading frame by introns are highly conserved in mouse TSP2 in comparison with mouse or human TSP1, a finding that suggests a close evolutionary relationship between the two genes. The TSP2 and TSP1 genes are also similar in that the 3[prime]-UTRs of both genes contain multiple TATT and ATTT(A) motifs that might function as mediators of mRNA stability. However, the sequences of the promoter regions in TSP1 and TSP2 are very different; in particular, the TSP2 gene lacks the serum response element and the NF-Y binding site that have been implicated in the serum response of the human TSP1 gene. The structure of the TSP2 gene is consistent with emerging evidence supporting the view that TSP1 and TSP2 perform overlapping but distinct functions. 41 refs., 4 figs., 1 tab.

Tetsuji Shingu; Bornstein, P. (Univ. of Washington, Seattle (United States))

1993-04-01T23:59:59.000Z

134

Low doses of neutrons induce changes in gene expression  

SciTech Connect

Studies were designed to identify genes induced in fibroblasts after exposure to low-dose neutron radiation but not after {gamma} rays. Our past work had shown similar modulation of transcripts for {alpha}-tubulin, {beta}- and {gamma}-actins, ornithine decarboxylase and interleukin 1 after exposure to either neutrons or {gamma} rays. However, differences in the expression of {beta}-protein kinase C and c-fos genes were observed, with both being induced after exposure to {gamma} rays but not neutrons. Recently, we have identified two genes that are induced after exposure to neutrons but not {gamma} rays: Rp-8 (a gene associated with apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency virus (HIV). Induction of Rp-8 mRNA was demonstrated in Syrian hamster embryo (SHE) fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.005 Gy/min) and high dose rate (0.12 Gy/min). No induction of other genes associated with apoptosis such as Rp-2, bcl-2 and Tcl-30 was observed. The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measurements of CAT activity and CAT transcripts after irradiation demonstrated an unresponsiveness to {gamma} rays over a broad range of doses (0.1-3 Gy). Twofold induction of the HIV-LTR was detected after exposure to neutrons (0.48 Gy) administered at low (0.05 Gy/min) but not high (0.12 Gy/min) dose rates. Ultraviolet-mediated HIV-LTR induction, however, was inhibited by exposure to low-dose-rate neutron irradiation. These results are interesting in light of reports that Rp-8 is induced during apoptosis and that HIV causes apoptosis. 17 refs., 3 figs., 1 tab.

Woloschak, G.E.; Chang-Liu, C.M. [Argonne National Lab., IL (United States); Panozzo, J.; Libertin, C.R. [Loyola Univ. of Chicago, Maywood, IL (United States)

1994-04-01T23:59:59.000Z

135

Energy Use in Chinese Buildings: Views of an Outsider Looking...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Energy Use in Chinese Buildings: Views of an Outsider Looking In Energy Use in Chinese Buildings: Views of an Outsider Looking In Energy Use in Chinese Buildings: Views of an...

136

Valley View Wind Farm | Open Energy Information  

Open Energy Info (EERE)

Wind Farm Wind Farm Jump to: navigation, search Name Valley View Wind Farm Facility Valley View Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner Juhl Wind Developer Valley View Transmission Energy Purchaser Xcel Energy Location Outside Chandler MN Coordinates 43.905808°, -96.020508° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":43.905808,"lon":-96.020508,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

137

states apps list view | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

states apps list view states apps list view States Data Apps Challenges Policies States You are here Data.gov » Communities » States List View Showing 1 - 5 of 5 results. Resources sort ascending Type Last Updated On TrashBackwards Trash Backwards will connect you with current recycling information, Do It Yourself reuse and upcycle tutorials that are easy to follow, reliable articles and infographics, upcycled products and durable alternatives to common single-use items, and links to non-profits and other groups who will benefit greatly from the stuff you're done with. Currently includes Massachusetts and Washington. Apps 02/27/2013 MoDOT's Traveler Information Map App With the launch of MoDOT's new Traveler Information Map mobile app, Missouri travelers can get the latest information on road conditions, work zones, flooding and incidents on their iPhones and Androids. Mobile 05/22/2013

138

Business Apps List View | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

Apps List View Apps List View BusinessUSA Data/Tools Apps Challenges Let's Talk BusinessUSA You are here Data.gov » Communities » BusinessUSA List View Showing 1 - 12 of 12 results. Resources sort ascending Type Last Updated On Traveling Entrepreneur Find green government opportunities for small businesses. The app based on your current location retrieves all the government programs and then allows you to share your favorite program on Facebook. Mobile 02/01/2012 Small Business Toolkit Small Business Toolbox provides a way to easily keep up-to-date on government programs, grants, awards, solicitations, and small business programs. Find the closest SBA district office, easily gathering license and permit information. Mobile 01/31/2012 SBIR.gov Awards Search This app allows users to search the SBIR awards database by agency, keyword, and year.

139

Repression of the Drosophila proliferating-cell nuclear antigen gene promoter by zerknuellt protein  

SciTech Connect

A 631-bp fragment containing the 5{prime}-flanking region of the Drosophila melanogaster proliferating-cell nuclear antigen (PCNA) gene was placed upstream of the chloramphenicol acetyltransferase (CAT) gene of a CAT vector. A transient expression assay of CAT activity in Drosophila Kc cells transfected with this plasmid and a set of 5{prime}-deletion derivatives revealed that the promoter function resided within a 192-bp region. Cotransfection with a zerknuellt (zen)-expressing plasmid specifically repressed CAT expression. However, cotransfection with expression plasmids for a nonfunctional zen mutation, even skipped, or bicoid showed no significant effect on CAT expression. RNase protection analysis revealed that the repression by zen was at the transcription step. The target sequence of zen was mapped within the 34-bp region of the PCNA gene promoter, even though it lacked zen protein-binding sites. Transgenic flies carrying the PCNA gene regulatory region fused with lacZ were established. These results indicate that zen indirectly represses PCNA gene expression, probably by regulating the expression of some transcription factor(s) that binds to the PCNA gene promoter.

Yamaguchi, Masamitsu; Hirose, Fumiko; Nishida, Yasuyoshi; Matsukage, Akio (Aichi Cancer Center Research Inst., Nagoya (Japan))

1991-10-01T23:59:59.000Z

140

Book review A Clear View of  

E-Print Network (OSTI)

Magazine R1 Book review A Clear View of the Cell Cycle James E. Ferrell, Jr. The Cell Cycle." It was a timely book, coming right on the heels of the discovery that cyclin-dependent kinases (Cdks) drive cell book. Enter "The Cell Cycle: Principles of Control", by David Morgan, just published by New Science

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141

Sharing the viewing experience through second screens  

Science Conference Proceedings (OSTI)

Despite the ever expanding forms of digital entertainment and the emergence of consumer recording facilities, allowing viewers to time shift their TV viewing habits, there are still certain TV shows and events that create an audience desire to be part ... Keywords: interactive, mobile, narrative, performance, second screen, shared experience, television, twitter

Mark Lochrie; Paul Coulton

2012-07-01T23:59:59.000Z

142

The view-selection problem has an exponential-time lower bound for conjunctive queries and views  

Science Conference Proceedings (OSTI)

The view-selection problem is to design and materialize a set of views over a database schema, such that the choice of views minimizes the cost of evaluating the selected workload of queries, and the combined size of the materialized views does not exceed ...

Rada Chirkova

2002-06-01T23:59:59.000Z

143

SOAJ Search : Main View : Deep Federated Search  

Office of Scientific and Technical Information (OSTI)

SOAJ Search SOAJ Search Search Powered By Deep Web Technologies New Search Preferences Powered by Deep Web Technologies HOME ABOUT ADVANCED SEARCH CONTACT US HELP Science Open Access Journals (SOAJ) Science Open Access Journals Main View This view is used for searching all possible sources. Additional Information Keyword: Title: Additional Information Author: Fields to Match: All Any Field(s) Additional Information Date Range: Beginning Date Range Pick Year 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 toEnding Date Range Pick Year 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 DWT Logo Search Clear All Help Simple Search Select All

144

Reasearch apps list view | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

Reasearch apps list view Reasearch apps list view Research Menu Data/Tools Apps Resources Let's Talk Research Alpha You are here Data.gov » Communities » Research Applications Showcase Showing 1 - 6 of 6 results. Resources sort ascending Type Last Updated On Wireless Spectrum Research & Development Senior Steering Group's Testbed Information Portal The Wireless Spectrum Research & Development Senior Steering Group's Testbed Information Portal provides information about spectrum testing facilities to government, academic, and industry researchers in need of such facilities. Apps 11/20/2012 The R&D Dashboard The R&D Dashboard beta website provides an initial look at U.S. Federal investments in science and research from two agencies: the National Institutes of Health (NIH) and the National Science Foundation (NSF) from years 2000-2009. The R&D Dashboard will expand in a future iteration to include ALL Federal research and development spending and expanded information on outputs. Apps 11/20/2012

145

consumer apps list view | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

consumer apps list view consumer apps list view Consumer Data Apps Challenges Resources About Blogs Let's Talk Feedback Consumer You are here Data.gov » Communities » Consumer Smart Disclosure Apps This page highlights apps and websites that use Smart Disclosure-style data to empower consumers to make better informed choices. The purpose is to illustrate the kinds of innovative apps-web- and mobile-based-that Smart Disclosure can fuel. The galleries below include both government-produced apps and apps created by innovators outside government that have won Federal challenges. Showing 1 - 19 of 19 results. Resources sort ascending Type Last Updated On Smart Traveler Smart Traveler, the official State Department app for U.S. travelers, invites you to see the world with easy access to frequently updated official country information, travel alerts, travel warnings, maps, U.S. embassy locations, and more. Mobile 09/14/2012

146

An nual En ergy Re view 2001  

Gasoline and Diesel Fuel Update (EIA)

An nual En ergy Re view 2001 An nual En ergy Re view 2001 The An nual En ergy Re view (AER) pres ents the En ergy In for ma tion Ad min - is tra tion's his tor i cal en ergy sta tis tics. For many se ries, sta tis tics are given for ev ery year from 1949 through 2001. The sta tis tics, ex pressed in ei ther phys i cal units or Brit ish ther mal units, cover all ma jor en ergy ac tiv i ties, in - clud ing con sump tion, pro duc tion, trade, stocks, and prices, for all ma jor en - ergy com mod i ties, in clud ing fos sil fu els, elec tric ity, and re new able en ergy sources. Pub li ca tion of this re port is re quired un der Pub lic Law 95-91 (De part ment of En ergy Or ga ni za tion Act), Sec tion 205(c), and is in keep ing with re spon - si bil i ties given to the En ergy In for ma tion Ad min is tra tion un der Sec tion

147

Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A  

SciTech Connect

The PTPA gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. PTPA, cloned from human genomic libraries, is encoded by one single-copy gene, composed of 10 exons and 9 introns with a total length of about 60 kb. The transcription start site was determined, and the 5{prime} flanking sequence was analyzed for its potential as a promotor. This region lacks a TATA sequence in the appropriate position relative to the transcription start, is very GC-rich, and contains upstream of the transcription start four Sp1 sites, a feature common to many TATA-less promotors. Based on the homology with DNA binding consensus sequences of transcription factors, we identified in this promotor region several putative DNA binding sites for transcription factors, such as NF-{kappa}B, Myb, Ets-1, Myc, and ATF. Transfection experiments with a construct containing the PTPA promotor region inserted 5{prime} of a luciferase reporter gene revealed that the 5{prime} flanking sequence of the PTPA gene indeed displayed promotor activity that seems to be cell-line dependent. By fluorescence in situ hybridization and G-banding, the PTPA gene was localized to the 9q34 region. The PTPA gene is positioned centromeric of c-abl in a region embracing several genes implicated in oncogenesis. 28 refs., 8 figs., 1 tab.

Van Hoof, C.; Cayla, X.; Merlevede, W.; Goris, J. [Katholieke Universiteit Leuven, Parish (France)] [and others] [Katholieke Universiteit Leuven, Parish (France); and others

1995-07-20T23:59:59.000Z

148

Proto-genes and de novo gene birth  

E-Print Network (OSTI)

Novel protein-coding genes can arise either through re-organization of pre-existing genes or de novo. Processes involving re-organization of pre-existing genes, notably after gene duplication, have been extensively described. ...

Carvunis, Anne-Ruxandra

149

Most Viewed Documents - Renewable Energy | OSTI, US Dept of Energy, Office  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Renewable Energy Most Viewed Documents - Renewable Energy Seventh Edition Fuel Cell Handbook NETL (2004) Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; et al. (1994) Metals design handbook Betts, W.S. (1988) Conduction heat transfer solutions VanSant, J.H. (1980) Lunar Wireless Power Transfer Feasibility Study Sheldon Freid, et al. (2008) The influence of grain size on the mechanical properties ofsteel Morris Jr., J.W. (2001) Fatigue design curves for 6061-T6 aluminum Yahr, G.T. (1993) Chapter 13. Absorption Refrigeration

150

April 2013 Most Viewed Documents for Renewable Energy Sources | OSTI, US  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Renewable Energy Sources April 2013 Most Viewed Documents for Renewable Energy Sources Chapter 11. Heat Exchangers Rafferty, Kevin D.; Culver, Gene (1998) 1252 Seventh Edition Fuel Cell Handbook NETL (2004) 628 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 290 Chapter 17. Engineering cost analysis Higbee, Charles V. (1998) 223 Geothermal Power Generation - A Primer on Low-Temperature, Small-Scale Applications Rafferty, K. (2000) 197 Calculation of brine properties. [Above 80/sup 0/F and for salt content between 5 and 25%] Dittman, G.L. (1977) 183 Chapter 13. Absorption Refrigeration Rafferty, Kevin D. (1998) 176 A study of lead-acid battery efficiency near top-of-charge and the impact on PV system design Stevens, J.W.; Corey, G.P. (1996) 173 Temperature coefficients for PV modules and arrays: Measurement

151

OpenEI:Neutral point of view | Open Energy Information  

Open Energy Info (EERE)

given to minor points of view. Neutral point of view is a principal core policy of the OpenEI platform. For further information, please refer to our model for this policy,...

152

JOBAID-VIEWING AN EMPLOYEE MATRIX (SUPERVISOR) | Department of...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

VIEWING AN EMPLOYEE MATRIX (SUPERVISOR) JOBAID-VIEWING AN EMPLOYEE MATRIX (SUPERVISOR) The purpose of this job aid is to guide supervisor users through the step-by-step process of...

153

Views and Patterns in E-Commerce Application Design  

Science Conference Proceedings (OSTI)

Separation of concerns is a well-established principle in software engineering that supports reuse by hiding complexity through abstraction mechanisms. The Abstract Design Views model was created with reuse in mind and allows the designer to apply separation ... Keywords: abstract design views, design, design patterns, object-oriented relationships, reuse, separation of concerns, software engineering, views

Marcus E. Markiewicz; Carlos J. P. Lucena; Paulo S. C. Alencar; Donald D. Cowan

2002-06-01T23:59:59.000Z

154

Mountain View IV | Open Energy Information  

Open Energy Info (EERE)

IV IV Facility Mountain View IV Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner AES Wind Generation Developer AES Wind Generation Energy Purchaser Southern California Edison Co Location White Water CA Coordinates 33.95475187°, -116.7015839° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":33.95475187,"lon":-116.7015839,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

155

Consumer Views on Transportation and Energy  

DOE Green Energy (OSTI)

This report has been assembled to provide the Office of Energy Efficiency and Renewable Energy (EERE) with an idea of how the American public views various transportation, energy, and environmental issues. An issue that still needs attention from EERE is the finding that the public tends to lack information about hybrid vehicles, hydrogen, and alternative fuels for passenger vehicles. Also, the public seems to want fuel-efficiency improvements and cleaner fuels, but is not very willing to pay for these benefits. The public also says that it supports initiatives to promote energy conservation over increased production and that it is willing to make changes such as driving less in an effort to reduce oil consumption.

Steiner, E.

2003-08-01T23:59:59.000Z

156

Human and Gorilla Genes  

NLE Websites -- All DOE Office Websites (Extended Search)

Human and Gorilla Genes Name: Eileen B Status: NA Age: NA Location: NA Country: NA Date: NA Question: What are the differences between the genetic mechanisms which affect...

157

Evaluation of ITER MSE Viewing Optics  

Science Conference Proceedings (OSTI)

The Motional Stark Effect (MSE) diagnostic on ITER determines the local plasma current density by measuring the polarization angle of light resulting from the interaction of a high energy neutral heating beam and the tokamak plasma. This light signal has to be transmitted from the edge and core of the plasma to a polarization analyzer located in the port plug. The optical system should either preserve the polarization information, or it should be possible to reliably calibrate any changes induced by the optics. This LLNL Work for Others project for the US ITER Project Office (USIPO) is focused on the design of the viewing optics for both the edge and core MSE systems. Several design constraints were considered, including: image quality, lack of polarization aberrations, ease of construction and cost of mirrors, neutron shielding, and geometric layout in the equatorial port plugs. The edge MSE optics are located in ITER equatorial port 3 and view Heating Beam 5, and the core system is located in equatorial port 1 viewing heating beam 4. The current work is an extension of previous preliminary design work completed by the ITER central team (ITER resources were not available to complete a detailed optimization of this system, and then the MSE was assigned to the US). The optimization of the optical systems at this level was done with the ZEMAX optical ray tracing code. The final LLNL designs decreased the ''blur'' in the optical system by nearly an order of magnitude, and the polarization blur was reduced by a factor of 3. The mirror sizes were reduced with an estimated cost savings of a factor of 3. The throughput of the system was greater than or equal to the previous ITER design. It was found that optical ray tracing was necessary to accurately measure the throughput. Metal mirrors, while they can introduce polarization aberrations, were used close to the plasma because of the anticipated high heat, particle, and neutron loads. These mirrors formed an intermediate image that then was relayed out of the port plug with more ideal (dielectric) mirrors. Engineering models of the optics, port plug, and neutral beam geometry were also created, using the CATIA ITER models. Two video conference calls with the USIPO provided valuable design guidelines, such as the minimum distance of the first optic from the plasma. A second focus of the project was the calibration of the system. Several different techniques are proposed, both before and during plasma operation. Fixed and rotatable polarizers would be used to characterize the system in the no-plasma case. Obtaining the full modulation spectrum from the polarization analyzer allows measurement of polarization effects and also MHD plasma phenomena. Light from neutral beam interaction with deuterium gas (no plasma) has been found useful to determine the wavelength of each spatial channel. The status of the optical design for the edge (upper) and core (lower) systems is included in the following figure. Several issues should be addressed by a follow-on study, including whether the optical labyrinth has sufficient neutron shielding and a detailed polarization characterization of actual mirrors.

Allen, S; Lerner, S; Morris, K; Jayakumar, J; Holcomb, C; Makowski, M; Latkowski, J; Chipman, R

2007-03-26T23:59:59.000Z

158

Two structurally distinct {kappa}B sequence motifs cooperatively control LPS-induced KC gene transcription in mouse macrophages  

SciTech Connect

The mouse KC gene is an {alpha}-chemokine gene whose transcription is induced in mononuclear phagocytes by LPS. DNA sequences necessary for transcriptional control of KC by LPS were identified in the region flanking the transcription start site. Transient transfection analysis in macrophages using deletion mutants of a 1.5-kb sequence placed in front of the chloramphenicol acetyl transferase (CAT) gene identified an LPS-responsive region between residues -104 and +30. This region contained two {kappa}B sequence motifs. The first motif (position -70 to -59, {kappa}B1) is highly conserved in all three human GRO genes and in the mouse macrophage inflammatory protein-2 (MIP-2) gene. The second {kappa}B motif (position -89 to -78, {kappa}B2) was conserved only between the mouse and the rat KC genes. Consistent with previous reports, the highly conserved {kappa}B site ({kappa}B1) was essential for LPS inducibility. Surprisingly, the distal {kappa}B site ({kappa}B2) was also necessary for optimal response; mutation of either {kappa}B site markedly reduced sensitivity to LPS in RAW264.7 cells and to TNF-{alpha} in NIH 3T3 fibroblasts. Although both {kappa}B1 and {kappa}B2 sequences were able to bind members of the Rel homology family, including NF{kappa}B1 (P50), RelA (65), and c-Rel, the {kappa}B1 site bound these factors with higher affinity and functioned more effectively than the {kappa}B2 site in a heterologous promoter. These findings demonstrate that transcriptional control of the KC gene requires cooperation between two {kappa}B sites and is thus distinct from that of the three human GRO genes and the mouse MIP-2 gene. 71 refs., 8 figs.

Ohmori, Y.; Fukumoto, S.; Hamilton, T.A. [Cleveland Clinic Foundation, Cleveland, OH (United States)

1995-10-01T23:59:59.000Z

159

Inhibition of interferon-inducible gene expression by adenovirus E1A proteins: Block in transcriptional complex formation  

SciTech Connect

Infection with wild-type adenovirus 5, but not with a mutant lacking the E1A gene, prevented the induction by interferon (IFN) {alpha} of chloramphenicol acetyltransferase (CAT) activity in HeLaM cell lines that had been permanently transfected with chimeric CAT reporter genes driven by the transcriptional regulatory regions of the IFN-inducible CAT activity was observed in cells that were cotransfected with the same reporter genes and plasmids expressing either the E1A 289- or 243-amino acid protein. These proteins also prevented the induction of CAT activity by IFN-{gamma} from a cotransfected HLA-DR{alpha}-CAT gene. Experiments with E1A mutants mapped the inhibitory activity to amino acid residues 38-65 of these proteins. In a HeLa cell line permanently expressing the E1A 289-amino acid protein, the replication of vesicular stomatitis virus and encephalomyocarditis virus was not inhibited by IFN-{alpha}, suggesting a global blockade of IFN responses. The observed transcriptional inhibition could be attributed to the lack of formation of the crucial IFN-stimulated gene factor 3 (ISGF3) transcriptional complex. As shown by mobility shift assays, this complex was not formed in the nuclear extracts of IFN-treated adenovirus-infected cells or IFN-treated E1A-producing cells. These nuclear extracts were deficient in both ISGF3{alpha} and ISGF3{gamma} subunits. However, they did not block the formation of ISGF3 complex from exogenously added components.

Kalvakolanu, D.V.R.; Bandyopadhyay, S.K.; Harter, M.L.; Sen, G.C. (Cleveland Clinic Foundation, OH (United States))

1991-09-01T23:59:59.000Z

160

Supply Chain - Additional Market Sectors View Page | Data.gov  

NLE Websites -- All DOE Office Websites (Extended Search)

Additional Market Sectors View Page Sustainable Supply Chains Submissions Let's Talk About Sustainable Supply Chain You are here Data.gov Communities Sustainable Supply Chain...

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

The Better Buildings Neighborhood View - December 10, 2013  

NLE Websites -- All DOE Office Websites (Extended Search)

traditional incandescent bulbs? Consider installing energy efficient compact fluorescent light bulbs (CFLs) in your basement lighting fixtures. Light Neighborhood View to Become...

162

September 2013 Most Viewed Documents for Energy Storage, Conversion...  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III...

163

`Trapped' Ions Provide First View of Light Property  

Science Conference Proceedings (OSTI)

... PHYSICS `Trapped' Ions Provide First View of Light Property. ... Quantum theory states that light can act either as a wave or as a particle. ...

164

April 2013 Most Viewed Documents for Fossil Fuels | OSTI, US...  

Office of Scientific and Technical Information (OSTI)

Viewed Documents for Fossil Fuels EXPERIMENTAL AND THEORETICAL DETERMINATION OF HEAVY OIL VISCOSITY UNDER RESERVOIR CONDITIONS Dr. Jorge Gabitto; Maria Barrufet (2003) 208 Fluid...

165

January 2013 Most Viewed Documents for Energy Storage, Conversion...  

Office of Scientific and Technical Information (OSTI)

January 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Energy Technology Perspectives 2012: Executive Summary Portuguese version NONE Energy...

166

Most Viewed Documents - Energy Storage, Conversion, and Utilization...  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Energy Storage, Conversion, and Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002)...

167

Most Viewed Documents by Subject Category | OSTI, US Dept of...  

Office of Scientific and Technical Information (OSTI)

Viewed Documents by Subject Category Biology and Medicine Chemistry Energy Storage, Conversion, and Utilization Engineering Environmental Sciences Fission and Nuclear Technologies...

168

Structure of the gene for the catalytic subunit of human DNA polymerase {delta} (POLD1)  

Science Conference Proceedings (OSTI)

We have isolated genomic DNA clones covering the gene for human DNA polymerase {delta} catalytic subunit (POLD1) and its 5{prime} flanking sequence. This gene is divided into 27 exons and is distributed over at least 32 kb of DNA. The exons and most of the introns are relatively small. The sizes of the exons range from 55 to 201 bp. Seven introns are smaller than 100 bp. Intron 1 is the largest intron, with a size of greater than 10 kb. All of the intron-exon junctions match well with the reported consensus sequence and the variable number of tandem repeats were found in several introns. Transcription of POLD1 appears to initiate at multiple sites. The major start site was 53 nucleotides upstream of the ATG start codon. The sequence of the promoter and upstream DNA is G+C rich and does not contain a TATA sequence. Several potential transcription factor-binding sites, including the AP2-, CTF-, Ets1-, GCF, MBF-1, NF-E1, and Sp1-binding sites, were found in this region. A 1.8-kb pol {delta} promoter DNA directed the expression of a luciferase reporter gene when transfected into HeLa cells. 69 refs., 4 figs., 3 tabs.

Chang, Lon-Sheng; Zhao, Lingyun; Zhu, Longyun [Ohio State Univ., Columbus, OH (United States)] [and others] [Ohio State Univ., Columbus, OH (United States); and others

1995-08-10T23:59:59.000Z

169

Microfluidic gene synthesis  

E-Print Network (OSTI)

The ability to synthesize custom de novo DNA constructs rapidly, accurately, and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications ...

Kong, David Sun, 1979-

2008-01-01T23:59:59.000Z

170

View discovery in OLAP databases through statistical combinatorial optimization  

SciTech Connect

OnLine Analytical Processing (OLAP) is a relational database technology providing users with rapid access to summary, aggregated views of a single large database, and is widely recognized for knowledge representation and discovery in high-dimensional relational databases. OLAP technologies provide intuitive and graphical access to the massively complex set of possible summary views available in large relational (SQL) structured data repositories. The capability of OLAP database software systems to handle data complexity comes at a high price for analysts, presenting them a combinatorially vast space of views of a relational database. We respond to the need to deploy technologies sufficient to allow users to guide themselves to areas of local structure by casting the space of 'views' of an OLAP database as a combinatorial object of all projections and subsets, and 'view discovery' as an search process over that lattice. We equip the view lattice with statistical information theoretical measures sufficient to support a combinatorial optimization process. We outline 'hop-chaining' as a particular view discovery algorithm over this object, wherein users are guided across a permutation of the dimensions by searching for successive two-dimensional views, pushing seen dimensions into an increasingly large background filter in a 'spiraling' search process. We illustrate this work in the context of data cubes recording summary statistics for radiation portal monitors at US ports.

Hengartner, Nick W [Los Alamos National Laboratory; Burke, John [PNNL; Critchlow, Terence [PNNL; Joslyn, Cliff [PNNL; Hogan, Emilie [PNNL

2009-01-01T23:59:59.000Z

171

Enabling device independent co-browsing through adaptive view points  

Science Conference Proceedings (OSTI)

Efficient co-browsing of web-pages from devices with different capabilities requires users to have a shared understanding of those web-pages. This paper outlines the concept of shared view point (SVP) and personal view point (PVP) for co-browsing before ... Keywords: Co-browsing, Device independence, Web content adaptation

H. N. Chua; S. D. Scott; Y. W. Choi

2008-03-01T23:59:59.000Z

172

The Determination of View-Factors in Urban Canyons  

Science Conference Proceedings (OSTI)

An important aspect of longwave radiation exchange within urban canopies is the degree to which the sky is obscured by surrounding buildings, a concept which may be described by the sky view-factor (?s). Hitherto, expressions for sky view-factors ...

Glenn T. Johnson; Ian D. Watson

1984-02-01T23:59:59.000Z

173

O'Leary outlines policy views at confirmation hearing  

Science Conference Proceedings (OSTI)

In her confirmation hearing, Secretary O'Leary outlined her views on various controversial issues. Among these were management of Yucca Mountain and the search for a nuclear waste repository, research on alternative fuels, encouragement of the use of renewable energy sources, and her views on intergrated resource planning.

Simpson, J.

1993-02-15T23:59:59.000Z

174

Is the Resource-Based View a Practical Organizational Theory?  

Science Conference Proceedings (OSTI)

We explore the question of whether the resource-based view (RBV) is a practical theory for managers. We test the ability of the RBV to provide two pieces of practical information: (1) the identification of the very few strategic assets from a large set ... Keywords: operational validity, resource-based view, simulation

Richard J. Arend; Moren Lévesque

2010-07-01T23:59:59.000Z

175

Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene  

Science Conference Proceedings (OSTI)

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) encodes two proteins that possess properties typical of single-stranded DNA-binding proteins (SSBs), late expression factor-3 (LEF-3), and a protein referred to as DNA-binding protein (DBP). Whereas LEF-3 is a multi-functional protein essential for viral DNA replication, transporting helicase into the nucleus, and forms a stable complex with the baculovirus alkaline nuclease, the role for DBP in baculovirus replication remains unclear. Therefore, to better understand the functional role of DBP in viral replication, a DBP knockout virus was generated from an AcMNPV bacmid and analyzed. The results of a growth curve analysis indicated that the dbp knockout construct was unable to produce budded virus indicating that dbp is essential. The lack of DBP does not cause a general shutdown of the expression of viral genes, as was revealed by accumulation of early (LEF-3), late (VP39), and very late (P10) proteins in cells transfected with the dbp knockout construct. To investigate the role of DBP in DNA replication, a real-time PCR-based assay was employed and showed that, although viral DNA synthesis occurred in cells transfected with the dbp knockout, the levels were less than that of the control virus suggesting that DBP is required for normal levels of DNA synthesis or for stability of nascent viral DNA. In addition, analysis of the viral DNA replicated by the dbp knockout by using field inversion gel electrophoresis failed to detect the presence of genome-length DNA. Furthermore, analysis of DBP from infected cells indicated that similar to LEF-3, DBP was tightly bound to viral chromatin. Assessment of the cellular localization of DBP relative to replicated viral DNA by immunoelectron microscopy indicated that, at 24 h post-infection, DBP co-localized with nascent DNA at distinct electron-dense regions within the nucleus. Finally, immunoelectron microscopic analysis of cells transfected with the dbp knockout revealed that DBP is required for the production of normal-appearing nucleocapsids and for the generation of the virogenic stroma.

Vanarsdall, Adam L. [Department of Microbiology, Nash Hall Room 220, Oregon State University, Corvallis, OR 97331-3804 (United States); Mikhailov, Victor S. [Department of Microbiology, Nash Hall Room 220, Oregon State University, Corvallis, OR 97331-3804 (United States); N.K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow 117808 (Russian Federation); Rohrmann, George F. [Department of Microbiology, Nash Hall Room 220, Oregon State University, Corvallis, OR 97331-3804 (United States)]. E-mail: rohrmanng@orst.edu

2007-08-01T23:59:59.000Z

176

Interaction of the 4S polycyclic hydrocarbon-binding protein with the cytochrome P450c gene  

SciTech Connect

The 4S polycyclic hydrocarbon binding protein has been purified from rat liver and its properties examined. The protein was incubated with subclones from the P450c gene; it specifically interacted with a plasmid that contained the 5'-half of intron 1, exon 1 and 5'-flanking sequences. Exonuclease foot-printing after binding of the 4S protein to portions of the P450c gene showed protection at -200 and -400 bp from exon 1. The region -882 to +2545bp was constructed before a reporter, chloramphenicol acetyl transferase (CAT) gene in a plasmid that contained the SV40 ori, polyA signals, ampicillin resist gene. The P450c region contained promoter and putative regulatory sequences. The construct was transfected into rat hepatocytes, RL-PR-C and into rat hepatoma cells, H-4-11-E. After addition of 3-methylcholanthrene (3MC), CAT expression was induced. When the plasmid was constructed with the P450c fragment inverted, no CAT expression was seen. Deletion of -95 to -665 or from -238 to -660 bp eliminated the expression of CAT in response to 3MC. These experiments indicated the importance of this region in the induction of P450c by 3MC.

Houser, W.H.; Cunningham, C.K.; Hines, R.N.; Bresnick, E.

1987-05-01T23:59:59.000Z

177

Science Conference Proceedings : Main View : Deep Web Business Search  

Office of Scientific and Technical Information (OSTI)

Main View Main View This view is used for searching all possible sources. A SCIENCE Accelerator Resource Additional Information Search: Search Clear All Conference Collections Welcome to the DOE Office of Scientific and Technical Information's (OSTI) Science Conference Proceedings Portal. This distributed portal provides access to science and technology conference proceedings and conference papers from a number of authoritative sites (professional societies and national labs, largely) whose areas of interest in the physical sciences and technology intersect those of the Department of Energy. Proceedings and papers from scientific meetings can be found in these fields, among others: particle physics, nuclear physics, chemistry, petroleum, aeronautics and astronautics, meteorology, engineering, computer science, electric power,

178

Prairie View Gas Recovery Biomass Facility | Open Energy Information  

Open Energy Info (EERE)

Prairie View Gas Recovery Biomass Facility Prairie View Gas Recovery Biomass Facility Jump to: navigation, search Name Prairie View Gas Recovery Biomass Facility Facility Prairie View Gas Recovery Sector Biomass Facility Type Landfill Gas Location St. Joseph County, Indiana Coordinates 41.6228085°, -86.3376761° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":41.6228085,"lon":-86.3376761,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

179

JOBAID-VIEWING USER RECORDS | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

INFORMATION SYSTEMS ANALYST E-mail arthur.johnson@hq.doe.gov Phone 202-586-9552 More Documents & Publications JOBAID-VIEWING AN EMPLOYEE MATRIX (SUPERVISOR) JOBAID-SELF REGISTERING...

180

PPPL's "Star Power" video offers compelling view of Laboratory...  

NLE Websites -- All DOE Office Websites (Extended Search)

PPPL's "Star Power" video offers compelling view of Laboratory's research By Jeanne Jackson DeVoe September 4, 2013 Tweet Widget Facebook Like Google Plus One PPPL has released its...

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

The Outlook for Energy - A View to 2030  

NLE Websites -- All DOE Office Websites (Extended Search)

The Outlook for Energy - A View to 2030 Speaker(s): David Reed Date: March 20, 2009 - 12:00pm Location: 90-3122 Seminar HostPoint of Contact: Jayant Sathaye Galen Barbose This...

182

February 2013 Most Viewed Documents for National Defense | OSTI...  

Office of Scientific and Technical Information (OSTI)

2013 Most Viewed Documents for National Defense Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 108 SMART BRIDGE:...

183

Real-Time Sky-View Factor Calculation and Approximation  

Science Conference Proceedings (OSTI)

Previously, the acquisition of sky-view factor data for climate studies has been time consuming and dependent on postprocessing. However, advances in technology now mean that techniques using fish-eye imagery can be algorithmically processed in ...

L. Chapman; J. E. Thornes

2004-05-01T23:59:59.000Z

184

Neutrons' view of hydrogen yields insight into HIV drug design...  

NLE Websites -- All DOE Office Websites (Extended Search)

Neutrons' view of hydrogen yields insight into HIV drug design ORNL-led study demonstrates relevance of neutrons in biomedical research An ORNL-led team used neutrons to study the...

185

Logan View Public Schools Wind Project | Open Energy Information  

Open Energy Info (EERE)

View Public Schools Wind Project View Public Schools Wind Project Jump to: navigation, search Name Logan View Public Schools Wind Project Facility Logan View Public Schools Sector Wind energy Facility Type Community Wind Location NE Coordinates 41.651546°, -96.50457° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":41.651546,"lon":-96.50457,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

186

Bartlett's Ocean View Wind Farm | Open Energy Information  

Open Energy Info (EERE)

Bartlett's Ocean View Wind Farm Bartlett's Ocean View Wind Farm Jump to: navigation, search Name Bartlett's Ocean View Wind Farm Facility Bartlett's Ocean View Wind Farm Sector Wind energy Facility Type Community Wind Facility Status In Service Owner Bartlett's Ocean View Wind Farm Energy Purchaser Bartlett's Ocean View Wind Farm Location Nantucket MA Coordinates 41.259168°, -70.131913° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":41.259168,"lon":-70.131913,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

187

Installing NikonView 4 under Windows (COOLPIX5000/995/885/775/990/880) A-1 Installing NikonView 4 underWindows  

E-Print Network (OSTI)

Installing NikonView 4 under Windows (COOLPIX5000/995/885/775/990/880) A-1 Installing NikonView 4 underWindows Installing Nikon View 4 for the COOLPIX5000/995/885/775/990/880 NikonView 4 includes- trator" (Windows 2000 Professional) or "ComputerAdministrator" (Win- dows XP Home Edition/Windows XP

Kleinfeld, David

188

Human gene encoding prostacyclin synthase (PTGIS): Genomic organization, chromosomal localization, and promoter activity  

Science Conference Proceedings (OSTI)

The prostacyclin synthase gene isolated from human genomic libraries (PTGIS) consists of 10 exons spanning approximately 60 kb. All the splice donor and acceptor sites conform to the GT/AG rule. Genomic Southern blot and fluorescence in situ hybridization analyses revealed that the human prostacyclin synthase gene is present as a single copy per haploid genome and is localized on chromosome 20q13.11-q13.13. The 1.5-kb sequence of the 5{prime} of the translational initiation site contained both GC-rich and pyrimidine-rich regions and consensus sequences of the transcription factor recognition sites such as Sp1, AP-2, the interferon-{gamma} response element, GATA, NF-{kappa}B, the CACCC box, and the glucocorticoid response element. The core binding sequence (GAGACC) of the shear stress responsive element was also found in the 5{prime}-flanking region of the gene. The major product of the primer extension analysis suggested that the transcription of the gene started from the positions around 49 bp upstream of the translational initiation codon. Transient transfection experiments using human aortic and bovine arterial endothelial cells demonstrated that the GC-rich region (positions -145 to -10) possessed a significant promoter activity. The 6-kb downstream sequence of the translational termination codon contained multiple polyadenylation signals, Alu repeat sequences, and the consensus sequence of the primate-repetitive DNA element, MER1. Two sizes of the prostacyclin synthase mRNAs (approximately 6 and 3.3 kb) were detected with the human aorta and lung. RNA blot hybridization analysis using the 3{prime}-untranslated region as probe indicated that the sizes of the 3{prime}-flanking regions were different in the major 6-kb and minor 3.3-kb mRNAs. 54 refs., 7 figs.

Yokoyama, Chieko; Yabuki, Tomoko; Inoue, Hiroyasu [National Cardiovascular Center Research Institute, Osaka (Japan)] [and others] [National Cardiovascular Center Research Institute, Osaka (Japan); and others

1996-09-01T23:59:59.000Z

189

Science Conference Proceedings : Main View : Deep Web Business Search  

Office of Scientific and Technical Information (OSTI)

Main View Main View This view is used for searching all possible sources. Additional Information Search: Title: Additional Information Author: Match: All Any Field(s) Additional Information Date Range: Beginning Date Range Pick Year 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 toEnding Date Range Pick Year 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 Search Clear All Notice to searchers: Due to the varied configuration and diversity of web pages and databases searched by Science Conference Proceedings, Full Record will search whatever data that is searchable at each site. Author and Title queries are only available on AIAA, AIP, ASCE, ASME, ECD,

190

Mountain View Electric Association, Inc - Energy Efficiency Credit Program  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Mountain View Electric Association, Inc - Energy Efficiency Credit Mountain View Electric Association, Inc - Energy Efficiency Credit Program Mountain View Electric Association, Inc - Energy Efficiency Credit Program < Back Eligibility Agricultural Commercial Industrial Residential Savings Category Appliances & Electronics Heating & Cooling Commercial Heating & Cooling Heat Pumps Commercial Lighting Lighting Manufacturing Water Heating Maximum Rebate LED Street Lighting: $20,000 LED Refrigerated Case Lighting Retrofit: $3,000 Commercial Lighting Replacement: $20,000 Program Info State Colorado Program Type Utility Rebate Program Rebate Amount Geothermal Heat Pumps: $150/ton, additional $150 per unit for Energy Star units greater than 3 tons, additional $120 if attached to electric water heater Air-Source Heat Pump: $125 - $150/ton, additional $100 - $150 per unit for

191

Tools for Viewing and Quality Checking ARM Data  

NLE Websites -- All DOE Office Websites (Extended Search)

Tools for Viewing and Quality Checking ARM Data Tools for Viewing and Quality Checking ARM Data S. Bottone and S. Moore Mission Research Corporation Santa Barbara, California Introduction Mission Research Corporation (MRC) is developing software tools to assist the Atmospheric Radiation Measurement (ARM) Data Quality Office with their data inspection tasks. One such tool is NCVweb, a web-based data analysis and visualization tool that allows for easy viewing of ARM NetCDF data files. This tool helps to eliminate the need of and problems associated with downloading large volumes of data, installing and configuring visualization software, or writing custom data exploration software. It has been upgraded this year with many new features described below. NCVweb can be used at the ARM Data Quality website directly via http://dq.arm.gov/ncvweb/ncvweb.cgi, or via the Data Quality

192

City of Mountain View, Missouri (Utility Company) | Open Energy Information  

Open Energy Info (EERE)

Mountain View Mountain View Place Missouri Utility Id 13057 Utility Location Yes Ownership M NERC Location SERC NERC SERC Yes Activity Distribution Yes References EIA Form EIA-861 Final Data File for 2010 - File1_a[1] LinkedIn Connections CrunchBase Profile No CrunchBase profile. Create one now! This article is a stub. You can help OpenEI by expanding it. Utility Rate Schedules Grid-background.png Residential Residential Average Rates Residential: $0.0810/kWh Commercial: $0.0807/kWh References ↑ "EIA Form EIA-861 Final Data File for 2010 - File1_a" Retrieved from "http://en.openei.org/w/index.php?title=City_of_Mountain_View,_Missouri_(Utility_Company)&oldid=409985" Categories: EIA Utility Companies and Aliases Utility Companies Organizations Stubs What links here

193

DOE Solicits Views on the Implementation of Large-Capacity Clothes...  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Solicits Views on the Implementation of Large-Capacity Clothes Washer Waivers DOE Solicits Views on the Implementation of Large-Capacity Clothes Washer Waivers November 30, 2010 -...

194

Evolution of Genes and Gene Networks in Filamentous Fungi  

E-Print Network (OSTI)

The Pezizomycotina, commonly known as the filamentous fungi, are a diverse group of organisms that have a major impact on human life. The filamentous fungi diverged from a common ancestor approximately 200 – 700 million years ago. Because of the diversity and the wealth of biological and genomic tools for the filamentous fungi it is possible to track the evolutionary history of genes and gene networks in these organisms. In this dissertation I focus on the evolution of two genes (lolC and lolD) in the LOL secondary metabolite gene cluster in Epichloë and Neotyphodium genera, the evolution of the MAP kinase-signaling cascade in the filamentous fungi, the regulation of the gene networks involved in asexual development in Neurospora crassa, and the identification of two genes in the N. crassa asexual development gene network, acon-2 and acon-3. I find that lolC and lolD originated as an ancient duplication in the ancestor of the filamentous fungi, which were later recruited in the LOL gene cluster in the fungal endophyte lineage. In the MAP kinase-signaling cascade, I find that the MAPK component is the most central gene in the gene network. I also find that the MAPK signaling cascade originated as three copies in the ancestor to eukaryotes, an arrangement that is maintained in filamentous fungi. My observations of gene expression profiling during N. crassa asexual development show tissue specific expression of genes. Both the vegetative mycelium and the aerial hyphae contribute to the formation of macroconidiophores. Also, with the help of genomic tools recently developed by researchers in the filamentous fungal community, I identified NCU00478 and NCU07617 as the genes with mutations responsible for two aconidial strains of N. crassa, acon-2 and acon-3 respectively.

Greenwald, Charles Joaquin

2010-08-01T23:59:59.000Z

195

Traits and Multiple Genes  

NLE Websites -- All DOE Office Websites (Extended Search)

Traits and Multiple Genes Traits and Multiple Genes Name: Frank Location: N/A Country: N/A Date: N/A Question: Please, could you give me an example of how human traits are controlled by more than one pair of alleles? Replies: Your question is just a bit vague, there are different answers depending on just what your question is. I will answer it in terms of polygenic traits also known as additive alleles. When you think of traits such as skin color, hair color and eye color, or traits where there is a wide range of phenotypes they are usually under the control of more than one pair of alleles. These alleles can even be on different chromosomes! Each pair of additive alleles adds to the phenotype. For instance in the case of skin color, scientists now believe that 3 genes control skin color. You then get 3 sets from your mother and 3 from your father for 6 possibilities. If all 6 of the alleles are for dark skin, you will have the darkest possible skin. If you have 5 dark alleles and one light, you will have very dark skin. If you have all 6 light alleles then you will have the lightest skin possible. Is it possible to have a child that is light skinned when both parents are dark-skinned? Well, not if both have all 6 dark alleles, but if they have some light alleles and the child inherits all of the possible light alleles available, then yes, the child could have lighter skin than either parent. It is now believed that eye color is not simply brown being dominant over blue because how many people do you know that have the same shade of brown or blue eyes? Eye color must also be polygenic.

196

Mechanisms of hormonal regulation of CAD gene expression and inhibition by Aryl hydrocarbon receptor agonist in human breast cancer cells  

E-Print Network (OSTI)

The CAD gene is trifunctional and expresses carbamoylphosphate synthetase/aspartate carbamyltransferase/dihydroorotase, which are required for pyrimidine biosynthesis. CAD gene activities are induced in MCF-7 human breast cancer cells, and treatment of MCF-7 or ZR-75 cells with 17b-estradiol (E2) resulted in a 3-5 fold increase in CAD mRNA levels in both cell lines. E2 induced reporter gene activity in MCF-7 and ZR-75 cells transfected with a construct containing the growth-responsive -90/+115 (pCAD1) region of the CAD gene promoter, which contains three upstream GC-rich and two downstream E-box motifs. Deletion and mutation analysis of the CAD gene promoter demonstrated that only the GC boxes that bind Sp1 protein were required for E2-responsiveness. Results of gel shift and chromatin immunoprecipitation (CHIP) assays show that both Sp1 and estrogen receptor a (ERa) interact with the GC-rich region of the CAD gene promoter. Moreover, hormone-induced transactivation of pCAD1 was inhibited by cotransfection with dominant-negative Sp1 expression plasmid and small inhibitory RNA for Sp1. These results demonstrate that, in common with many other genes involved in E2-induced cell proliferation, the CAD gene is also regulated by a nonclassical ERa/Sp1-mediated pathway. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands suppress several E2-induced responses in the rodent uterus and mammary tumors and in human breast cancer cells. TCDD inhibited hormone-induced activation of CAD mRNA levels and reporter gene activity in MCF-7 and ZR-75 cells transfected with E2-responsive pCAD promoter constructs. E2-mediated transactivation of pCAD constructs with a mutant inhibitory dioxin responsive element DRE (iDRE) were also inhibited by TCDD suggesting that inhibitory AhR-ERa/Sp1 crosstalk was iDRE-independent. It was not possible to determine whether the levels of ERa in cells cotreated with E2 plus TCDD were limiting since the proteasome inhibitor MG132 itself directly decreased CAD mRNA levels. Using fluorescence resonance energy transfer (FRET), it was shown that both E2 and TCDD enhanced AhR-ERa interactions. E2 also induced interactions between ERa and Sp1. However cotreatment with TCDD abrogated this effect. Results of this study demonstrate a unique model of AhR-ERa crosstalk where the liganded AhR inhibits ERa-Sp1 interactions and also recruits ERa to Ahresponsive gene promoters such as CYP1A1.

Khan, Shaheen Munawar Ali

2005-12-01T23:59:59.000Z

197

Analysis of travel review data from reader's point of view  

Science Conference Proceedings (OSTI)

In the NLP field, there have been a lot of works which focus on the reviewer's point of view conducted on sentiment analyses, which ranges from trying to estimate the reviewer's score. However the reviews are used by the readers. The reviews that give ...

Maya Ando; Shun Ishizaki

2012-07-01T23:59:59.000Z

198

Asia View / November 2007 / Page 1 Rajat Ganguly  

E-Print Network (OSTI)

Asia View / November 2007 / Page 1 Rajat Ganguly Pakistan, the only Muslim country in the world so far with nuclear weapons, is today one of the most dangerous places on earth. To be sure, politics in Pakistan has always been turbulent. But what's new about the situation today is that the country

199

Multi-view gender classification using hierarchical classifiers structure  

Science Conference Proceedings (OSTI)

In this paper, we propose a hierarchical classifier structure for gender classification based on facial images by reducing the complexity of the original problem. In the proposed framework, we first train a classifier, which will properly divide the ... Keywords: gender classification, hierarchical classifiers, multi-view facial images

Tian-Xiang Wu; Bao-Liang Lu

2010-11-01T23:59:59.000Z

200

Rigorous Development of Dependable Systems Using Fault Tolerance Views  

Science Conference Proceedings (OSTI)

This paper introduces the Mode and Fault Tolerance Views approach to stepwise rigorous development of critical systems. It supports systematic, structured and recursive modelling of system fault tolerance, including error detection, error recovery and ... Keywords: formal methods, Event-B, fault tolerance, modal systems, case study, AOCS

Ilya Lopatkin; Alexei Iliasov; Alexander Romanovsky

2011-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

Lithium Hexamethyldisilazide: A View of Lithium Ion Solvation  

E-Print Network (OSTI)

Lithium Hexamethyldisilazide: A View of Lithium Ion Solvation through a Glass-Bottom Boat BRETT L and reactivities, we were drawn to lithium hexamethyldisilazide (LiHMDS; (Me3Si)2NLi) by its promi- nence principles of lithium ion coordination chemistry.2 Understanding how solvation influences organolithium

Collum, David B.

202

Multiversion Based View Maintenance Over Distributed Data Sources  

E-Print Network (OSTI)

. Categories and Subject Descriptors: H.2.4 [Database Management]: Systems--Distributed databases; Relational on Database Systems, Vol. x, No. x, 12 2004, Pages 0­30. #12;Multiversion Based View Maintenance over maintenance prototype system. Experiments comparing mainte- nance performance with and without TxnWrap enabled

203

Action Refinement from a Logical Point of View  

Science Conference Proceedings (OSTI)

Action refinement provides a mechanism to design a complex reactive system hierarchically. This paper is devoted to action refinement from a logical point of view, and to combining the hierarchical implementation of a complex system with the hierarchical ... Keywords: action refinement, modal logics, reactive systems, specification, verification

Mila E. Majster-Cederbaum; Naijun Zhan; Harald Fecher

2002-01-01T23:59:59.000Z

204

news and views A key issue for hydrogen storage materi-  

E-Print Network (OSTI)

news and views A key issue for hydrogen storage materi- als is that the hydrogenation and dehydro be possible to discover stable hydrogen hydrates with higher storage Hydrogen Posture Plan www.eere.energy.gov/hydrogenandfuelcells/pdfs/ hydrogen_posture_plan.pdf 7. Kuhs, W

Palumbi, Stephen

205

Book ReViews Crystal Growth Technology: From Fundamentals and  

E-Print Network (OSTI)

Book ReViews Crystal Growth Technology: From Fundamentals and Simulation to Large-Scale Production-3-527-31762-2. This book contains 19 selected reviews from the Third International Workshop on Crystal Growth Technology been used with narrower margins, thereby maintaining the same number of pages. This book would

Regel, Liya L.

206

Book ReViews Comprehensive Cellulose Chemistry. Volume 1. Fundamentals  

E-Print Network (OSTI)

Book ReViews Comprehensive Cellulose Chemistry. Volume 1. Fundamentals and Analytical Methods. By D is a valuable and much-needed reference book for both the novice and the practitioner of cellulose chemistry does this significantly reduce the clarity of the book. The section on cellulose structure is extensive

Dantus, Marcos

207

Interface between Microbiology and Structural Biology as viewed by NMR  

E-Print Network (OSTI)

1 Interface between Microbiology and Structural Biology as viewed by NMR Muriel DELEPIERRE, Anne NMR spectroscopy is one of two principal experimental techniques used in structural biology. It can and intermolecular interactions. We aim to give an overview of the use of modern high resolution NMR methodology

Paris-Sud XI, Université de

208

GeneDistiller—Distilling Candidate Genes from Linkage Intervals  

E-Print Network (OSTI)

Background: Linkage studies often yield intervals containing several hundred positional candidate genes. Different manual or automatic approaches exist for the determination of the gene most likely to cause the disease. While the manual search is very flexible and takes advantage of the researchers ’ background knowledge and intuition, it may be very cumbersome to collect and study the relevant data. Automatic solutions on the other hand usually focus on certain models, remain ‘‘black boxes’ ’ and do not offer the same degree of flexibility. Methodology: We have developed a web-based application that combines the advantages of both approaches. Information from various data sources such as gene-phenotype associations, gene expression patterns and protein-protein interactions was integrated into a central database. Researchers can select which information for the genes within a candidate interval or for single genes shall be displayed. Genes can also interactively be filtered, sorted and prioritised according to criteria derived from the background knowledge and preconception of the disease under scrutiny. Conclusions: GeneDistiller provides knowledge-driven, fully interactive and intuitive access to multiple data sources. It displays maximum relevant information, while saving the user from drowning in the flood of data. A typical query takes less than two seconds, thus allowing an interactive and explorative approach to the hunt for the candidate gene.

Dominik Seelow; Jana Marie Schwarz; Markus Schuelke

2008-01-01T23:59:59.000Z

209

Recessive and Dominant Gene Action  

NLE Websites -- All DOE Office Websites (Extended Search)

Recessive and Dominant Gene Action Recessive and Dominant Gene Action Name: Katie Location: N/A Country: N/A Date: N/A Question: What Causes some genes to be ressesive and other genes to be dominant? Replies: Think about the fact that genes code for directions for making proteins. There are many different kinds of proteins in our body-enzymes for regulating metabolism, structural proteins for building our bodies, hormones for regulating processes etc. If the gene for the protein is structural, then it is important to have the right kind and the right amount. If the gene is defective (usually recessive genes are defective, but not always) the right protein will not be made and the structure will either be defective and won't work at all or there won't be enough to maintain the structure. Sometimes you need both genes to be working to get enough of the structure. So a homozygous person will have the strongest structure. A heterozygote would have one gene that is working a may produce enough of the protein to maintain the structure, but maybe not. So in some cases, just having one copy of the dominant (working) gene is enough. If it is a trait for something like eye color, this is not going to cause a defect, just a difference. In this case, being heterozygous or homozgyous for the dominant trait produces the same color of eye. In the case of sickle cell anemia, the recessive gene changes the protein structure of the shape of the red blood cell. If you have one good copy and one bad copy of the gene, some of your cells will be normal and some will be sickle cells. One good copy of the gene gives you enough normal red blood cells to stay healthy. But if you don't have a normal copy, all of your cells have the capability to sickle under certain conditions and this can be fatal. So think of dominant and recessive genes in terms of what they produce and what that protein is supposed to do and then think of what would happen if the dominant gene was able to overtake the effect of the recessive gene.

210

The BAT1 gene in the MHC encodes an evolutionarily conserved putative nuclear RNA helicase of the DEAD family  

Science Conference Proceedings (OSTI)

The BAT1 gene has previously been identified about 30 kb upstream from the tumor necrosis factor (TNF) locus and close to a NF{sub kb}-related gene of the nuclear factor family in the major histocompatibility complex (MHC) of human, mouse, and pig. We now show that the BAT1 translation product is the homolog of the rat p47 nuclear protein, the WM6 Drosophila gene product, and probably also Ce08102 of Caenorhabditis elegans, all members of the DEAD protein family of ATP-dependent RNA helicases. This family has more than 40 members, including the eukaryotic translation initiation factor-4A (eIF-4A), the human nuclear protein p68, and the Drosophila oocyte polar granule component vasa. BAT1 spans about 10 kb, is split into 10 exons of varying length, and encodes a protein of 428 amino acids ({approximately}48 kDa). Human and pig BAT1 cDNAs display 95.6% identity in the coding region and 80% identity in the 5{prime} and 3{prime} noncoding regions. Several repeat sequences of different types were identified in introns of the porcine BAT1 gene. Three different mRNAs, 4.1,1.7, and 0.9 kb, respectively, were detected in all tissues analyzed upon hybridization with porcine BAT1 cDNA. Transfection and expression of human BAT1 cDNA after tagging with a heterologous antibody recognition epitope revealed a nuclear localization of the hybrid protein. An MspI RFLP was detected in an SLA class I typed family, confirming the localization of the BAT1 gene in the porcine MHC. BAT1 thus encodes a putative nuclear ATP-dependent RNA helicase and is likely to have an indispensable function. 35 refs., 6 figs., 1 tab.

Peelman, L.J.; Van Zeveren, A.; Coppeiters, W. [State Univ. Ghent, Merelbeke (Belgium)] [and others

1995-03-20T23:59:59.000Z

211

PlantView® OMX Web Services & Web Portal Version 1.5  

Science Conference Proceedings (OSTI)

The PlantView/OMX Portal V1.5.0.0 is a web site to view PlantView® data that is designed to be configured and modified by end-user. It can display data in different ways; with PlantView® data from various ...

2013-08-07T23:59:59.000Z

212

VIEWS ON U.S. WATER RESEARCH AND TECHNOLOGY TRANSFER PROGRAMS  

E-Print Network (OSTI)

contributions. The problem is that research is often viewed as an esoteric activity involving scientists who

District of Columbia, University of the

213

Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices  

E-Print Network (OSTI)

Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices *

Hoen, Ben

2010-01-01T23:59:59.000Z

214

Deferred incremental refresh of XML materialized views: algorithms and performance evaluation  

Science Conference Proceedings (OSTI)

The view mechanism can provide the user with an appropriate portion of database through data filtering and integration. Views are often materialized for query performance improvement, and in that case, their consistency needs to be maintained against ... Keywords: XML, deferred incremental view refresh, materialized view, semistructured data

Hyunchul Kang; Hosang Sung; ChanHo Moon

2003-01-01T23:59:59.000Z

215

The Honorable Gene Dean  

Office of Legacy Management (LM)

Energy' .' Energy' .' Washington, DC 20585 DE! 14 1gg4 The Honorable Gene Dean \ P.O. Box 1659~ L Huntington, West Virginia 25717 _ ',.. : Dear Mayor Dean: Secretary of Energy Hazel O'Leary has'announced,a new approach ,to openness in the'Department of Energy (DOE) and its communications with the public. In support of this in.itiative, we are pleased,to forward the..enclosed information related to the former Reduction Pilot Plant sitein your jurisdiction that performed work for DOE predecessor agencies. This information is provided.for your information, use,.band retention., DDE's Formerly Utilized Sites Remedial.Action Program is responsible for identification of sitesused by DOE's predecessor agencies, determini,ng theirs current radiological condition and, where it has authority, performing

216

September 2013 Most Viewed Documents for Energy Storage, Conversion, And  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for Energy Storage, Conversion, And September 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 169 Evaluation of the 2010 Toyota Prius Hybrid Synergy Drive System Burress, Timothy A [ORNL]; Campbell, Steven L [ORNL]; Coomer, Chester [ORNL]; Ayers, Curtis William [ORNL]; Wereszczak, Andrew A [ORNL]; Cunningham, Joseph Philip [ORNL]; Marlino, Laura D [ORNL]; Seiber, Larry Eugene [ORNL]; Lin, Hua-Tay [ORNL] (2011) 116 Evaluation of the 2007 Toyota Camry Hybrid Syneregy Drive System Burress, T.A.; Coomer, C.L.; Campbell, S.L.; Seiber, L.E.; Marlino, L.D.; Staunton, R.H.; Cunningham, J.P. (2008) 102 A study of lead-acid battery efficiency near top-of-charge and the impact on PV system design

217

Most Viewed Documents - Mathematics and Computing | OSTI, US Dept of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Mathematics and Computing Most Viewed Documents - Mathematics and Computing Metaphors for cyber security. Moore, Judy Hennessey; Parrott, Lori K.; Karas, Thomas H. (2008) Staggered-grid finite-difference acoustic modeling with the Time-Domain Atmospheric Acoustic Propagation Suite (TDAAPS). Aldridge, David Franklin; Collier, Sandra L. (U.S. Army Research Laboratory); Marlin, David H. (U.S. Army Research Laboratory); et al. (2005) Salinas : theory manual. Walsh, Timothy Francis; Reese, Garth M.; Bhardwaj, Manoj Kumar (2004) LANL Site Update Armstrong, Harold G [Los Alamos National Laboratory] (2012) Scientific Data Management Integrated Software Infrastructure Center (SDM/ISIC): Scientific Process Automation (SPA), FINAL REPORT Bertram Ludaescher; Ilkay Altintas (2012) HPC Utilities

218

Mountain View Elec Assn, Inc | Open Energy Information  

Open Energy Info (EERE)

Mountain View Elec Assn, Inc Mountain View Elec Assn, Inc Place Colorado Utility Id 13058 Utility Location Yes Ownership C NERC Location WECC NERC WECC Yes Activity Distribution Yes References EIA Form EIA-861 Final Data File for 2010 - File1_a[1] LinkedIn Connections CrunchBase Profile No CrunchBase profile. Create one now! This article is a stub. You can help OpenEI by expanding it. Utility Rate Schedules Grid-background.png 16.01 RESIDENTIAL RATE Residential 16.05 RESIDENTIAL TIME OF DAY SERVICE RATE Residential 18.40 SMALL POWER RATE Commercial 18.60 LARGE POWER RATE Commercial 18.61 LARGE POWER - PRIMARY METERING RATE Commercial 18.62 LARGE POWER - LOAD MANAGEMENT RATE Commercial 18.63 LARGE POWER - LOAD MANAGEMENT - PRIMARY METERING RATE Commercial 18.64 GENERAL POWER RATE Industrial

219

April 2013 Most Viewed Documents for Power Generation And Distribution |  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Power Generation And Distribution April 2013 Most Viewed Documents for Power Generation And Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; Traczyk, P.A. (1994) 719 Seventh Edition Fuel Cell Handbook NETL (2004) 628 ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) 343 Wet cooling towers: rule-of-thumb design and simulation Leeper, S.A. (1981) 290 Load flow analysis: Base cases, data, diagrams, and results Portante, E.C.; Kavicky, J.A.; VanKuiken, J.C.; Peerenboom, J.P. (1997) 248 Controlled low strength materials (CLSM), reported by ACI Committee 229 Rajendran, N. (1997) 106 Micro-CHP Systems for Residential Applications Timothy DeValve; Benoit Olsommer (2007)

220

The Collagen Protein Viewed at Unprecedented Detail | Advanced Photon  

NLE Websites -- All DOE Office Websites (Extended Search)

Assembling Nanoparticles the Easy DNA-Way Assembling Nanoparticles the Easy DNA-Way Better, cleaner fuel injectors for automobiles? Poxvirus Potency Uncovered in New Atomic Map Striking Nano Gold Oldest Known Magnet's Secrets Revealed Under High Pressures Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed The Collagen Protein Viewed at Unprecedented Detail FEBRUARY 26, 2008 Bookmark and Share A view of a rat tail tendon using second-harmonic generation microscopy. The collagen fibers show up in green and red. The structure and behavior of one of the most common proteins in our bodies has been resolved at a level of detail never before seen, thanks to new research performed at the Advanced Photon Source (APS) at the U.S.

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

City View, South Carolina: Energy Resources | Open Energy Information  

Open Energy Info (EERE)

View, South Carolina: Energy Resources View, South Carolina: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 34.8615064°, -82.4315121° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":34.8615064,"lon":-82.4315121,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

222

E-print Network : Main View : Deep Federated Search  

Office of Scientific and Technical Information (OSTI)

E-print Network E-print Network Search Powered By Deep Web Technologies New Search Preferences E-print Network E-print Network Skip to main content FAQ * HELP * SITE MAP * CONTACT US Home * About * Advanced Search * Browse by Discipline * Scientific Societies * E-print Alerts * Add E-prints Powered by Deep Web Technologies E-print Network E-print Network Skip to main content FAQ * HELP * SITE MAP * CONTACT US Home * About * Advanced Search * Browse by Discipline * Scientific Societies * E-print Alerts * Add E-prints Main View This view is used for searching all possible sources. Due to the varied configuration and diversity of web pages and databases searched by E-prints, Full Record will search whatever data is searchable at each site. Multiple arXiv sites under one general heading are combined

223

Standard specification for illuminators used for viewing industrial radiographs  

E-Print Network (OSTI)

1.1 The function of the illuminator is to provide sufficient illumination and viewing capabilities for the purpose of identification and interpretation of radiographic images. This specification provides the recommended minimum requirements for Industrial Radiographic Illuminators used for viewing industrial radiographic films using transmitted light sources. 1.2 The illuminator has to ensure the same safety for personnel, or users of any electric apparatus, as specified by electrical standards applicable in the country in which the illuminator is used. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1.3 Values stated in SI units are to be regarded as the standard. The values given in parenthesis are provided for information only.

American Society for Testing and Materials. Philadelphia

2006-01-01T23:59:59.000Z

224

Narrow Field of View Zenith Radiometer (NFOV) Handbook  

SciTech Connect

The two-channel narrow field-of-view radiometer (NFOV2) is a ground-based radiometer that looks straight up and measures radiance directly above the instrument at wavelengths of 673 and 870 nm. The field-of-view of the instrument is 1.2 degrees, and the sampling time resolution is one second. Measurements of the NFOV2 have been used to retrieve optical properties for overhead clouds that range from patchy to overcast. With a one-second sampling rate of the NFOV2, faster than almost any other ARM Climate Research Facility (ACRF) instrument, we are able, for the first time, to capture changes in cloud optical properties at the natural time scale of cloud evolution.

Chiu, C; Marshak, A; Hodges, G; Barnard, JC; Schmelzer, J

2008-11-01T23:59:59.000Z

225

East Pleasant View, Colorado: Energy Resources | Open Energy Information  

Open Energy Info (EERE)

View, Colorado: Energy Resources View, Colorado: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 39.7276261°, -105.1573284° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":39.7276261,"lon":-105.1573284,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

226

April 2013 Most Viewed Documents for Energy Storage, Conversion, And  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Energy Storage, Conversion, And April 2013 Most Viewed Documents for Energy Storage, Conversion, And Utilization Seventh Edition Fuel Cell Handbook NETL (2004) 628 Continuously variable transmissions: theory and practice Beachley, N.H.; Frank, A.A. (null) 205 A study of lead-acid battery efficiency near top-of-charge and the impact on PV system design Stevens, J.W.; Corey, G.P. (1996) 173 Energy Saving Potentials and Air Quality Benefits of Urban HeatIslandMitigation Akbari, Hashem (2005) 153 Building a secondary containment system Broder, M.F. (1994) 144 An Improved Method of Manufacturing Corrugated Boxes: Lateral Corrugator Frank C. Murray Ph.D.; , Roman Popil Ph.D.; Michael Shaepe (formerly with IPST, now at Cargill. Inc) (2008) 141 Ammonia usage in vapor compression for refrigeration and air-conditioning in the United States

227

Most Viewed Documents - Energy Storage, Conversion, and Utilization | OSTI,  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Energy Storage, Conversion, and Utilization Most Viewed Documents - Energy Storage, Conversion, and Utilization Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) Continuously variable transmissions: theory and practice Beachley, N.H.; Frank, A.A. () Review of air flow measurement techniques McWilliams, Jennifer (2002) Building a secondary containment system Broder, M.F. (1994) Cost benefit analysis of the night-time ventilative cooling in office building Seppanen, Olli; Fisk, William J.; Faulkner, David (2003) Evaluation of the 2007 Toyota Camry Hybrid Syneregy Drive System Burress, T.A.; Coomer, C.L.; Campbell, S.L.; et al. (2008) Nanofluid technology : current status and future research. Choi, S. U.-S. (1998) An Improved Method of Manufacturing Corrugated Boxes: Lateral

228

Most Viewed Documents - Power Generation and Distribution | OSTI, US Dept  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Power Generation and Distribution Most Viewed Documents - Power Generation and Distribution Electric power high-voltage transmission lines: Design options, cost, and electric and magnetic field levels Stoffel, J.B.; Pentecost, E.D.; Roman, R.D.; et al. (1994) ASPEN Plus Simulation of CO2 Recovery Process Charles W. White III (2003) Systems and economic analysis of microalgae ponds for conversion of CO{sub 2} to biomass. Quarterly technical progress report, September 1993--December 1993 Benemann, J.R.; Oswald, W.J. (1994) Load flow analysis: Base cases, data, diagrams, and results Portante, E.C.; Kavicky, J.A.; VanKuiken, J.C.; et al. (1997) Multilevel converters -- A new breed of power converters Lai, J.S. [Oak Ridge National Lab., TN (United States). Engineering Technology Div.]; Peng, F.Z. [Univ. of Tennessee, Knoxville, TN (United

229

National Library of Energy : Main View : Deep Federated Search  

Office of Scientific and Technical Information (OSTI)

National Library of Energy National Library of Energy Search Powered By Deep Web Technologies Modify Search Preferences Powered by Deep Web Technologies HOME About NLE Communications FAQs Help Site Map Feedback/Contact Us Main View This view is used for searching all possible sources. SEARCH OPTIONS Additional Information Keyword: Title: Additional Information Author: Fields to Match: All Any Field(s) Additional Information Date Range: Beginning Date Range Pick Year 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 toEnding Date Range Pick Year 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 Search Clear All Help Simple Search Expand Category All Sources

230

Most Viewed Documents - Environmental Sciences | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Environmental Sciences Most Viewed Documents - Environmental Sciences Separation of heavy metals: Removal from industrial wastewaters and contaminated soil Peters, R.W.; Shem, L. (1993) Special Report on Emissions Scenarios : a special report of Working Group III of the Intergovernmental Panel on Climate Change Nakicenovic, Nebojsa; Alcamo, Joseph; Davis, Gerald; et al. (2000) CARBON DIOXIDE (REDUCTION) FUJITA,E. (2000) Ecological Screening Values for Surface Water, Sediment, and Soil Friday, G. P. (1999) Mitigation options for accidental releases of hazardous gases Fthenakis, V.M. (1995) Atmospheric Chemistry and Greenhouse Gases Ehhalt, D.; Prather, M.; Dentener, F.; et al. (2001) Ammonia usage in vapor compression for refrigeration and air-conditioning in the United States Fairchild, P.D.; Baxter, V.D. (1995)

231

April 2013 Most Viewed Documents for Fission And Nuclear Technologies |  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Fission And Nuclear Technologies April 2013 Most Viewed Documents for Fission And Nuclear Technologies Behavior of spent nuclear fuel in water pool storage Johnson, A.B. Jr. (null) 298 Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. (null) 292 Graphite design handbook Ho, F.H. (1988) 216 System Definition and Analysis: Power Plant Design and Layout NONE (1996) 123 Flow-induced vibration of circular cylindrical structures Chen, S.S. (1985) 116 Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste] Rezvani, M.A.; Ziada, H.H. (Westinghouse Hanford Co., Richland, WA (United States)); Shurrab, M.S. (Westinghouse Savannah River Co., Aiken, SC (United

232

EM SSAB Contributes Community Views to Clean-up Decisions  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Contributes Community Views to Clean-up Decisions Contributes Community Views to Clean-up Decisions 2011 Local Board Contributions The Environmental Management Site-Specific Advisory Board (EM SSAB) has a unique mandate to provide input regarding the cleanup of nuclear legacy sites to the Department of Energy's Office of Environmental Management (EM). Chartered under the Federal Advisory Committee Act, the EM SSAB today comprises eight local boards, located in Hanford, WA; Idaho Falls, ID: Los Alamos, NM; the Nevada National Security Site; Oak Ridge, TN; Paducah, KY; Portsmouth, OH; and the Savannah River Site, SC. EM has made public participation a fundamental component of its cleanup mission and has found that the EM SSAB, which draws upon diverse community

233

Field Guide: Live Working Rope (Optimized for Electronic Viewing)  

Science Conference Proceedings (OSTI)

Ropes are an essential tool in many applications associated with the electric power industry. Ropes used in proximity to or in contact with high-voltage power lines require demanding dielectric properties as well as strength and durability. A comprehensive understanding of live working rope use is necessary for safe and efficient operations wherever such ropes are used. This field guide has been optimized for viewing on electronic devices. For a copy of this product printed on high-quality paper and ring...

2011-12-21T23:59:59.000Z

234

Consumer Views on Transportation and Energy (Second Edition)  

DOE Green Energy (OSTI)

This report has been assembled to provide the Office of Energy Efficiency and Renewable Energy (EERE) with an idea of how the American public views various transportation, energy, and environmental issues. The data presented in this report have been drawn from multiple sources: surveys conducted by the Opinion Research Corporation (ORC) for the National Renewable Energy Laboratory (NREL) that are commissioned and funded by EERE, Gallup polls, and other sources.

Kubik, M.

2005-04-01T23:59:59.000Z

235

GenePRIMP: A GENE PRediction IMprovement Pipeline for Prokaryotic genomes  

Science Conference Proceedings (OSTI)

We present 'gene prediction improvement pipeline' (GenePRIMP; http://geneprimp.jgi-psf.org/), a computational process that performs evidence-based evaluation of gene models in prokaryotic genomes and reports anomalies including inconsistent start sites, missed genes and split genes. We found that manual curation of gene models using the anomaly reports generated by GenePRIMP improved their quality, and demonstrate the applicability of GenePRIMP in improving finishing quality and comparing different genome-sequencing and annotation technologies.

Pati, Amrita; Ivanova, Natalia N.; Mikhailova, Natalia; Ovchinnikova, Galina; Hooper, Sean D.; Lykidis, Athanasios; Kyrpides, Nikos C.

2010-04-01T23:59:59.000Z

236

Genomic structure and chromosomal mapping of the human CD22 gene  

SciTech Connect

The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. A nearly full length human CD22 cDNA clone was used to isolate genomic clones that span the CD22 gene. The CD22 gene is spread over 22 kb of DNA and is composed of 15 exons. The first exon contains the major transcriptional start sites. The translation initiation codon is located in exon 3, which also encodes a portion of the signal peptide. Exons 4 to 10 encode the seven Ig domains of CD22, exon 11 encodes the transmembrane domain, exons 12 to 15 encode the intracytoplasmic domain of CD22, and exon 15 also contains the 3' untranslated region. A minor form of CD22 mRNA likely results from splicing of exon 5 to exon 8, skipping exons 6 and 7. A 4.6-kb Xbal fragment of the CD22 gene was used to map the chromosomal location of CD22 by fluorescence in situ hybridization. The hybridization locus was identified by combining fluorescent images of the probe with the chromosomal banding pattern generated by an Alu probe. The results demonstrate the CD22 is located within the band region q13.1 of chromosome 19. Two closely clustered major transcription start sites and several minor start sites were mapped by primer extension. Similarly to many other lymphoid-specific genes, the CD22 promoter lacks an obvious TATA box. Approximately 4 kb of DNA 5' of the transcription start sites were sequenced and found to contain multiple Alu elements. Potential binding sites for the transcriptional factors NF-kB, AP-1, and Oct-2 are located within 300 bp 5' of the major transcription start sites. A 400-bp fragment (bp -339 through +71) of the CD22 promoter region was subcloned into a pGEM-chloramphenicol acetyltransferase vector and after transfection into B and T cells was found to be active in both B and T cells. 45 refs., 7 figs., 2 tabs.

Wilson, G.L.; Kozlow, E.; Kehrl, J.H. (National Institutes of Health, Bethesda, MD (United States)); Najfeld, V. (Mount Sinai Medical Center, New York, NY (United States)); Menniger, J.; Ward, D. (Yale School of Medicine, New Haven, CT (United States))

1993-06-01T23:59:59.000Z

237

What is the morphology of a gene?  

NLE Websites -- All DOE Office Websites (Extended Search)

of as structural, operator, and regulatory genes." - Dorland's Illustrated Medical Dictionary, 27th ed. So, in terms of morphology, a gene is a specific sequence of nucleotide in...

238

Gene encoding herbicide safener binding protein  

DOE Patents (OSTI)

The cDNA encoding safener binding protein (SafBP), also referred to as SBP1, is set forth in FIG. 5 and SEQ ID No. 1. The deduced amino acid sequence is provided in FIG. 5 and SEQ ID No. 2. Methods of making and using SBP1 and SafBP to alter a plant's sensitivity to certain herbicides or a plant's responsiveness to certain safeners are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors and seeds from said plants.

Walton, Jonathan D. (East Lansing, MI); Scott-Craig, John S. (East Lansing, MI)

1999-01-01T23:59:59.000Z

239

Wind energy. Views on the environment: clean and green  

DOE Green Energy (OSTI)

As the United States grapples with the issue of global climate change resulting from fossil fuel combustion, and as the U.S. Congress and individual state legislatures consider restructuring the electric utility industry, lawmakers should keep in mind the environmental preferability of renewable energy sources such as wind and the long, continuing record of public support for them. This is particularly important in view of restructuring, which will have the effect of shifting decisions about the type and quantity of new power plants to be built from utility executives to the general public. Preliminary information suggests that ''green,'' or environmentally-friendly, power sources could win a significant market share. In addition to creating new demand for clean energy sources, this development is likely to create a committed, educated political constituency for clean energy that has not existed in the past. In such an altered environment for the selection of new generation, public attitudes on the desirability of various power sources will become much more important than they have in the past. The purpose of this paper is to briefly summarize public opinion surveys on the environment in general, renewable energy in general, and wind energy in particular in that order, using data gathered from polling in the U.S., the United Kingdom, and Canada. At this writing, more than 16 years after the first wind plants began going up in California, there is a solid and growing body of information available on public acceptance of wind energy. This paper draws on more than 25 surveys conducted over the years on wind and renewables, as well as individual findings on attitudes on the environment from other polls. An abbreviated summary of the public attitudes reviewed in this document is as follows: Views on the Environment: Public concern about protecting the environment, and particularly those aspects of the environment that relate to human health, such as air pollution, is high and growing. Views on Renewable Energy: The public strongly prefers using a combination of efficiency and renewables to meet energy needs, and has done so in poll after poll taken since the late 1970s. Views on Wind, General: The bulk of public opinion on wind is from the U.K., where wind development in recent years has been active and controversial. In more than a dozen surveys taken over the past five years, respondents have expressed strong backing for wind. In addition, support for wind has typically strengthened after a wind plant has been installed and operating for some time. Views on Wind, Specific: Public polling information on wind energy's specific attributes is also primarily from the U.K. Surveys found that wind's primary positive points are that it is clean, renewable, safe, and helps conserve fossil fuels; its principal weaknesses are the fact that it is intermittent and somewhat more expensive than fossil-fired power. With respect to wind's environmental impacts, those surveyed were most concerned by visual impact and noise. However, in virtually every poll, the number of respondents expressing negative views is very small.

Gray, Thomas O.

1999-12-01T23:59:59.000Z

240

Ground control failures. A pictorial view of case studies  

Science Conference Proceedings (OSTI)

The book shows, in pictorial views, many forms and/or stages of types of failures in mines, for instance, cutter, roof falls, and cribs. In each case, the year of occurrence is stated in the beginning so that the environment or technological background under which it occurred are reflected. The narrative than begins with the mining and geological conditions, followed by a description of the ground control problems and recommended solutions and results, if any. The sections cover failure of pillars, roof falls, longwall, roof bolting, multiple-seam mining, floor heave, longwall, flooding and weathering of coal, old workings, and shortwall and thin-seam plow longwall.

Peng, S.S.

2007-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Prodigal: Microbial Gene Prediction Software  

NLE Websites -- All DOE Office Websites (Extended Search)

Screenshot Screenshot Artemis Screenshot of Prodigal Results Compared with Curated Annotations and other Computational Genefinders for Anaeromyxobacter dehalogenans 2CP-C (Click to enlarge) Prodigal (Prokaryotic Dynamic Programming Genefinding Algorithm) is a microbial (bacterial and archaeal) gene finding program developed at Oak Ridge National Laboratory and the University of Tennessee. Key features of Prodigal include: Speed: Prodigal is an extremely fast gene recognition tool (written in very vanilla C). It can analyze an entire microbial genome in 30 seconds or less. Accuracy: Prodigal is a highly accurate gene finder. It correctly locates the 3' end of every gene in the experimentally verified Ecogene data set (except those containing introns). It possesses a very

242

Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on  

NLE Websites -- All DOE Office Websites (Extended Search)

6: March 20, 6: March 20, 2006 Consumer Views on Gasoline Taxes to someone by E-mail Share Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes on Facebook Tweet about Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes on Twitter Bookmark Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes on Google Bookmark Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes on Delicious Rank Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes on Digg Find More places to share Vehicle Technologies Office: Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes on AddThis.com... Fact #416: March 20, 2006 Consumer Views on Gasoline Taxes

243

Mountain View Power Partners I Wind Farm | Open Energy Information  

Open Energy Info (EERE)

I Wind Farm I Wind Farm Jump to: navigation, search Name Mountain View Power Partners I Wind Farm Facility Mountain View Power Partners I Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner MDU Resources Developer SeaWest Energy Purchaser L.A. Department of Water Resources Location San Gorgonio CA Coordinates 33.9095°, -116.734° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":33.9095,"lon":-116.734,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

244

Psychological Projection: Islamic and Freudian Views Published by Administrator  

E-Print Network (OSTI)

Projection is a term applied by Sigmund Freud for the act of projecting one’s feelings, attitudes, etc. onto other people. Projection is one of many defense mechanisms, another term for the strategies people use to cope with situations which arouse anxiety. To make this clearer, let us consider an example. A person who always lies, according to Freud, is more likely to see others as liars too. The same is true for a thief; he might feel vigilant about the safety of his own property because he projects his bad deeds onto other people, thinking that other people may have higher tendencies to steal. How does Islam view the idea of psychological projection developed by Freud? First of all, it is appropriate to highlight the writings of Said Nursi in which he tells the story of two travelers with different states in their hearts. One was serene and optimistic and viewed the world in positive ways while the other one was anxious and pessimistic, seeing the world as all negative. Both went to similar places, where ordinary lifestyles could be observed. The former one saw the place as beautiful with many good things existing there. However, it was different for the latter; wherever he looked he saw negative things. According to Bediuzzaman Said Nursi, this difference of perspective between these two

Taufik Bin Mohammad

2013-01-01T23:59:59.000Z

245

Efficient view-dependent out-of-core visualization  

E-Print Network (OSTI)

Hierarchical levels of details (HLODs) have proven to be an efficient way to visualize complex environments and models even in an out-of-core system. Large objects are partitioned into a spatial hierarchy and on each node a level of detail is generated for efficient view-dependent rendering. To ensure correct matching between adjacent nodes in the hierarchy care has to be taken to prevent cracks along the cuts. This either leads to severe simplification constraints at the cuts and thus to a significantly higher number of triangles or the need for a costly runtime stitching of these nodes. In this paper we present an out-of-core visualization algorithm that overcomes this problem by filling the cracks generated by the simplification algorithm with appropriately shaded fat borders. Furthermore, several minor yet important improvements of previous approaches are made. This way we come up with a simple nevertheless efficient view-dependent rendering technique which allows for the natural incorporation of state-of-the-art culling, simplification, compression and prefetching techniques. 1.

Michael Guthe; Pavel Borodin; Reinhard Klein

2003-01-01T23:59:59.000Z

246

A motion sensor interactive interface for viewing and manipulating protein structural data in 3D  

Science Conference Proceedings (OSTI)

We propose a fun, interactive way to view and alter protein structural data by hand via motion sensors and voice activation. This will enable users to freely zoom, rotate, and view a protein through a friendly hands-on experience. Multiple view and rotation ...

Robyn Moncrief; William Gobber

2012-08-01T23:59:59.000Z

247

ViewPointer: lightweight calibration-free eye tracking for ubiquitous handsfree deixis  

Science Conference Proceedings (OSTI)

We introduce ViewPointer, a wearable eye contact sensor that detects deixis towards ubiquitous computers embedded in real world objects. ViewPointer consists of a small wearable camera no more obtrusive than a common Bluetooth headset. ViewPointer allows ... Keywords: attentive user interface, eye tracking

John D. Smith; Roel Vertegaal; Changuk Sohn

2005-10-01T23:59:59.000Z

248

Mathematical modeling and information theoretical analysis of DevR regulated genes in Mycobacterium tuberculosis  

E-Print Network (OSTI)

The DevR-DevS two component system of Mycobacterium tuberculosis is responsible for its dormancy in host and becomes operative under hypoxic condition. It is experimentally known that phosphorylated DevR controls the expression of several downstream genes in a complex manner. In the present work we have developed a mathematical model to show the role of binding sites in the DevR mediated gene expression. Through modeling it has been shown the individual and collective role of the binding sites in regulating the DevR mediated gene expression. The objective of the present work is two fold. First, to describe qualitatively the temporal dynamics of wild type genes and their known mutants. Based on these results we propose that DevR controlled gene expression follows a specific pattern which is efficient in describing other DevR mediated gene expression. Second, to analyze the behavior of the system from the information theoretical point of view. Using the tools of information theory we have calculated the molecular efficiency of the system and have shown that it is close to the maximum limit of isothermal efficiency.

Arnab Bandyopadhyay; Soumi Biswas; Alok Kumar Maity; Suman K Banik

2013-09-03T23:59:59.000Z

249

Gene order computation using Alzheimer's DNA microarray gene expression data and the ant colony optimisation algorithm  

Science Conference Proceedings (OSTI)

As Alzheimer's Disease (AD) is the most common form of dementia, the study of AD-related genes via biocomputation is an important research topic. One method of studying AD-related gene is to cluster similar genes together into a gene order. Gene ...

Chaoyang Pang; Gang Jiang; Shipeng Wang; Benqiong Hu; Qingzhong Liu; Youping Deng; Xudong Huang

2012-11-01T23:59:59.000Z

250

City of Lake View, Iowa (Utility Company) | Open Energy Information  

Open Energy Info (EERE)

Place Iowa Place Iowa Utility Id 10617 Utility Location Yes Ownership M NERC Location MRO NERC MRO Yes ISO MISO Yes Activity Buying Transmission Yes Activity Distribution Yes Activity Bundled Services Yes References EIA Form EIA-861 Final Data File for 2010 - File1_a[1] LinkedIn Connections CrunchBase Profile No CrunchBase profile. Create one now! This article is a stub. You can help OpenEI by expanding it. Utility Rate Schedules Grid-background.png Commercial Service Commercial Residential Service Residential Rural Service Residential Three-Phase Service Industrial Average Rates Residential: $0.0698/kWh Commercial: $0.0685/kWh Industrial: $0.0710/kWh References ↑ "EIA Form EIA-861 Final Data File for 2010 - File1_a" Retrieved from "http://en.openei.org/w/index.php?title=City_of_Lake_View,_Iowa_(Utility_Company)&oldid=409821

251

Mountain View Power Partners III Wind Farm | Open Energy Information  

Open Energy Info (EERE)

Power Partners III Wind Farm Power Partners III Wind Farm Facility Mountain View Power Partners III Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner PPM Energy Inc Developer PPM Energy Inc Energy Purchaser San Diego Gas & Electric Location San Gorgonio CA Coordinates 33.9095°, -116.734° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":33.9095,"lon":-116.734,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

252

National Synchrotron Light Source A View of Brookhaven  

NLE Websites -- All DOE Office Websites (Extended Search)

managed for the U.S. Department of Energy managed for the U.S. Department of Energy by Brookhaven Science Associates, a company founded by Stony Brook University and Battelle managed for the U.S. Department of Energy by Brookhaven Science Associates, a company founded by Stony Brook University and Battelle National Synchrotron Light Source A View of Brookhaven Brookhaven National Laboratory is a multipurpose re- search laboratory funded by the U.S. Department of En- ergy. Located on a 5,300-acre site on Long Island, New York, the Laboratory operates large-scale facilities for studies in physics, chemistry, biology, medicine, applied science, and advanced technology. Brookhaven's 2,600 scientists, engineers, and support staff are joined each year by more than 5,000 visiting research- ers from around the world.

253

Mountain View Power Partners II Wind Farm | Open Energy Information  

Open Energy Info (EERE)

Power Partners II Wind Farm Power Partners II Wind Farm Facility Mountain View Power Partners II Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner MDU Resources Developer SeaWest Energy Purchaser L.A. Department of Water Resources Location San Gorgonio CA Coordinates 33.9095°, -116.734° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":33.9095,"lon":-116.734,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

254

Prosection views: Dimensional inference through sections and projections  

E-Print Network (OSTI)

We present some basic properties of two general graphical techniques for constructing views of high-dimensional objects, projection and section. Projections can easily display aspects of structure that are only of low dimension, while sections, i.e., intersections of subspaces with a highdimensional object, can easily display structure of only low codimension (and hence often high dimension). However, compositions of sections and projections, here called prosections, can display aspects of structure of any intermediate dimension. These statements are relevant for data analysis: projections of data can be easily generated with x-yscatterplots, 3-D data rotations, and grand tours, while sections can be approximated in existing systems by scatterplot brushing and painting. Thus this paper is in part an investigation into the principles underlying these techniques.

George W. Furnas; Andreas Buja

1994-01-01T23:59:59.000Z

255

Metazoan Gene Families from Metazome  

DOE Data Explorer (OSTI)

Metazome is a joint project of the Department of Energy's Joint Genome Institute and the Center for Integrative Genomics to facilitate comparative genomic studies amongst metazoans. Clusters of orthologous and paralogous genes that represent the modern descendents of ancestral gene sets are constructed at key phylogenetic nodes. These clusters allow easy access to clade specific orthology/paralogy relationships as well as clade specific genes and gene expansions. As of version 2.0.4, Metazome provides access to twenty-four sequenced and annotated metazoan genomes, clustered at nine evolutionarily significant nodes. Where possible, each gene has been annotated with PFAM, KOG, KEGG, and PANTHER assignments, and publicly available annotations from RefSeq, UniProt, Ensembl, and JGI are hyper-linked and searchable. The included organisms (by common name) are: Human, Mouse, Rat, Dog, Opossum, Chicken, Frog, Stickleback, Medaka, Fugu pufferfish; Zebrafish, Seasquirt - savignyi, Seasquirt - intestinalis, Amphioxus, Sea Urchin, Fruitfly, Mosquite, Yellow Fever Mosquito, Silkworm, Red Flour Beetle, Worm, Briggsae Worm, Owl limpet (snail), and Sea anemone. [Copied from Metazome Overview at http://www.metazome.net/Metazome_info.php

256

ParaView on the Data Analytics Cluster on Eureka/Gadzooks | Argonne  

NLE Websites -- All DOE Office Websites (Extended Search)

on the Data Analytics Cluster on Eureka/Gadzooks on the Data Analytics Cluster on Eureka/Gadzooks Set Up User Environment The recommended way of running ParaView on Eureka is in client/server mode. This consists of running the ParaView client on your local resource, and the ParaView server (pvserver) on the Eureka visualization nodes. The latest version currently installed on Eureka is ParaView 4.0.1. This should first be installed on your local resource. Binary and source packages for Linux, MacOS, and Windows are available from the ParaView Download Page. (Run the 'softenv' command on the Eureka login node to see additional versions of ParaView that are available on Eureka). To put ParaView in your environment on Eureka, add the following line to your ~/.softenvrc file NOTE: This must go before the @default entry. Also note that this macro

257

T-538: HP OpenView Storage Data Protector Bug Lets Remote Users Execute  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

T-538: HP OpenView Storage Data Protector Bug Lets Remote Users T-538: HP OpenView Storage Data Protector Bug Lets Remote Users Execute Arbitrary Code T-538: HP OpenView Storage Data Protector Bug Lets Remote Users Execute Arbitrary Code January 20, 2011 - 6:39am Addthis PROBLEM: HP OpenView Storage Data Protector Bug Lets Remote Users Execute Arbitrary Code. PLATFORM: HP OpenView Storage Data Protector v6.11 ABSTRACT: A vulnerability was reported in HP OpenView Storage Data Protector. A remote user can execute arbitrary code on the target system. reference LINKS: HP Security Bulletin SecurityTracker Alert ID: 1024983 CVE-2011-0273 IMPACT ASSESSMENT: High Discussion: A potential security vulnerability has been identified with HP OpenView Storage Data Protector. The vulnerability could be remotely exploited to execute arbitrary code.

258

T-721:Mac OS X Directory Services Lets Local Users View User Password  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

21:Mac OS X Directory Services Lets Local Users View User 21:Mac OS X Directory Services Lets Local Users View User Password Hashes T-721:Mac OS X Directory Services Lets Local Users View User Password Hashes September 20, 2011 - 8:45am Addthis PROBLEM: Mac OS X Directory Services Lets Local Users View User Password Hashes. PLATFORM: Mac OS X Lion (10.7) ABSTRACT: A local user can view user password hashes. reference LINKS: SecurityTracker Alert ID: 1026067 Apple Support Downloads Apple Security Updates Apple OS X Lion v10.7.1 Update IMPACT ASSESSMENT: Medium Discussion: A vulnerability was reported in Mac OS X. A local user can view user password hashes. A local user can invoke the following Directory Services command line command to view the password hash for the target user: dscl localhost -read /Search/Users/[target user] A local user can change their

259

Helix Wind Inc formerly ClearView Acquisitions | Open Energy Information  

Open Energy Info (EERE)

Helix Wind Inc formerly ClearView Acquisitions Helix Wind Inc formerly ClearView Acquisitions Jump to: navigation, search Name Helix Wind Inc. (formerly ClearView Acquisitions) Place San Diego, California Zip 92113 Sector Wind energy Product California-based manufacturer of small scale wind turbines. References Helix Wind Inc. (formerly ClearView Acquisitions)[1] LinkedIn Connections CrunchBase Profile No CrunchBase profile. Create one now! This article is a stub. You can help OpenEI by expanding it. Helix Wind Inc. (formerly ClearView Acquisitions) is a company located in San Diego, California . References ↑ "Helix Wind Inc. (formerly ClearView Acquisitions)" Retrieved from "http://en.openei.org/w/index.php?title=Helix_Wind_Inc_formerly_ClearView_Acquisitions&oldid=346471"

260

Regulation of E2F-1 gene expression in human breast cancer cells  

E-Print Network (OSTI)

17?-Estradiol induces E2F-1 gene expression in ZR-75 and MCF-7 human breast cancer cells. Analysis of the E2F-1 gene promoter in MCF-7 cells previously showed that hormone-induced transactivation required interactions between estrogen receptor ? (ER?)/Sp1 bound to upstream GC-rich sites and NFYA bound to downstream CCAAT sites within the -169 to -54 promoter region. This promoter region was also E2-responsive in ER?-positive ZR-75 cells; however, further analysis of the promoter showed that cooperative ER?/Sp1/NFY interactions were not necessary for hormone-induced transactivation in ZR-75 cells. The upstream GC-rich motifs are activated independently by ER?/Sp1 in ZR-75 but not MCF-7 cells, and the downstream CCAAT sites were also E2-responsive. E2 also induced reporter gene activity in ZR-75 cells transfected with an expression plasmid containing the yeast GAL4 DNA binding domain fused to pM-NFYA and a construct containing five tandem GAL4 response elements. Subsequent studies showed that hormonal activation of pE2F-1jm1 and pM-NFYA are dependent on non-genomic pathways in which E2 activates cAMP/protein kinase A. Hormone-dependent regulation of E2F-1 gene expression in ZR-75 and MCF-7 involves different mechanisms, demonstrating the importance of cell context on transactivation pathways, even among ER-positive breast cancer cell lines. TCDD inhibited ER?-mediated responses in MCF-7 and ZR-75 cells. E2- induced E2F-1protein and mRNA levels in MCF-7 and ZR-75 cells and this response was inhibited by TCDD. Constructs containing GC-rich sites alone or in combination with the downstream NFY sites were used in transactivation studies to investigate the mechanism of inhibitory AhR-ER? crosstalk. Although TCDD inhibited E2-induced mRNA, protein and reporter gene actitivity, it was not possible to determine if the inhibitory response was due to limiting ER? protein levels due to proteasome degradation since proteaome inhibitors alone blocke hormone-dependent responses. TCDD also inhibited the cAMP/PKA pathway by inhibiting adenyl cyclase activity. In Drosophila SL-2 cells cotransfected with the GC-rich -169 to -54 region, ER? and Sp1 plasmids E2 induced transactivation in cells cotransfected with AhR/Arnt expression plasmids suggesting that the AhR complex suppressed ER?/Sp1 action. These results demonstrate that TCDD inhibits E2-dependent activation of both non-genomic and genomic pathways of ER-mediated E2F-1 gene expression. 17?-Estradiol induces E2F-1 gene expression in ZR-75 and MCF-7 human breast cancer cells. Analysis of the E2F-1 gene promoter in MCF-7 cells previously showed that hormone-induced transactivation required interactions between estrogen receptor ? (ER?)/Sp1 bound to upstream GC-rich sites and NFYA bound to downstream CCAAT sites within the -169 to -54 promoter region. This promoter region was also E2-responsive in ER?-positive ZR-75 cells; however, further analysis of the promoter showed that cooperative ER?/Sp1/NFY interactions were not necessary for hormone-induced transactivation in ZR-75 cells. The upstream GC-rich motifs are activated independently by ER?/Sp1 in ZR-75 but not MCF-7 cells, and the downstream CCAAT sites were also E2-responsive. E2 also induced reporter gene activity in ZR-75 cells transfected with an expression plasmid containing the yeast GAL4 DNA binding domain fused to pM-NFYA and a construct containing five tandem GAL4 response elements. Subsequent studies showed that hormonal activation of pE2F-1jm1 and pM-NFYA are dependent on non-genomic pathways in which E2 activates cAMP/protein kinase A. Hormone-dependent regulation of E2F-1 gene expression in ZR-75 and MCF-7 involves different mechanisms, demonstrating the importance of cell context on transactivation pathways, even among ER-positive breast cancer cell

Ngwenya, Sharon Khethiwe

2003-05-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

A human parvovirus, adeno-associated virus, as a eucaryotic vector: Transient expression and encapsidation of the procaryotic gene for chloramphenicol acetyltransferase  

SciTech Connect

The authors have used the defective human parvovirus adeno-associated virus (AAV) as a novel eurocaryotic vector (parvector) for the expression of a foreign gene in human cells. The recombinant, pAV2, contains the AAV genome in a pBR322-derived bacterial plasmid. When pAV2 is transfected into human cells together with helper adenovirus particles, the AAV genome is rescued from the recombinant plasmid and replicated to produce infectious AAV particles at high efficiency. To create a vector, we inserted a procaryotic sequence coding for chloramphenicol acetyltransferase (CAT) into derivatives of pAV2 following either of the AAV promoters p/sub 40/ (pAVHiCAT) and p/sub 19/ (pAVBcCAT). When transfected into human 293 cells or HeLa cells, pAVHiCAT expressed CAT activity in the absence of adenovirus. In the presence of adenovirus, this vector produced increased amounts of CAT activity and the recombinant AAV-CAT genome was replicated. In 293 cells, pAVBcCAT expressed a similar amount of CAT activity in the absence or presence of adenovirus and the recombinant AAV-CAT genome was not replicated. In HeLa cells, pAVBcCAT expressed low levels of CAT activity, but this level was elevated by coinfection with adenovirus particles or by cotransfection with a plasmid which expressed the adenovirus early region 1A (E1A) product. The E1A product is a transcriptional activator and is expressed in 293 cells. Thus, expression from two AAV promoters is differentially regulated: expression from p/sub 19/ is increased by E1A, whereas p/sub 40/ yields high levels of constitutive expression in the absence of E1A. Both AAV vectors were packaged into AAV particles by complementation with wild-type AAV and yielded CAT activity when subsequently infected into cells in the presence of adenovirus.

Tratschin, J.D.; West, M.H.P.; Sandbank, T.; Carter, B.J.

1984-10-01T23:59:59.000Z

262

THE JOURNAL OF GENE MEDICINE RESEARCH ARTICLE J Gene Med 2008; 10: 583592.  

E-Print Network (OSTI)

-transduction with the two types of viral vectors provided doxycycline- regulated transgene expression in a neuron gene transfer; tetracycline-regulated gene expression; CNS; lentiviral vectors; doxycycline; cell

Bristol, University of

263

A simple Bayesian estimate of direct RNAi gene regulation events from differential gene expression profiles  

E-Print Network (OSTI)

,13]. * Correspondence: paul.a.wilson@gsk.com 1Computational Biology, GlaxoSmithKline Medicine Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK Full list of author information is available at the end of the article Wilson and Plucinski BMC Genomics 2011, 12... differential expression profile. Hierarchical clustering and heat map representations of the most differentially expressed tran- scripts (Additional File 1 Figure S9) suggest that the KSHV-miR-k12-11 transfected dataset is more similar to the control data than...

Wilson, Paul A; Plucinski, Mathew

2011-05-20T23:59:59.000Z

264

Gene expression by software mechanisms  

Science Conference Proceedings (OSTI)

This paper describes the molecular interactions and coordination of cell processes using computer operating system concepts related to synchronization and communication. We argue that in molecular biology, the genes and their chromatin context provide ... Keywords: cell biology, modules, operating systems, pipe, process communication, signals

Gabriel Ciobanu; Bogdan Tanasa

2002-01-01T23:59:59.000Z

265

Viewing spin structures with soft x-ray microscopy  

SciTech Connect

The spin of the electron and its associated magnetic moment marks the basic unit for magnetic properties of matter. Magnetism, in particular ferromagnetism and antiferromagnetism is described by a collective order of these spins, where the interaction between individual spins reflects a competition between exchange, anisotropy and dipolar energy terms. As a result the energetically favored ground state of a ferromagnetic system is a rather complex spin configuration, the magnetic domain structure. Magnetism is one of the eldest scientific phenomena, yet it is one of the most powerful and versatile utilized physical effects in modern technologies, such as in magnetic storage and sensor devices. To achieve highest storage density, the relevant length scales, such as the bit size in disk drives is now approaching the nanoscale and as such further developments have to deal with nanoscience phenomena. Advanced characterization tools are required to fully understand the underlying physical principles. Magnetic microscopes using polarized soft X-rays offer a close-up view into magnetism with unique features, these include elemental sensitivity due to X-ray magnetic dichroism effects as contrast mechanism, high spatial resolution provided by state-of-the-art X-ray optics and fast time resolution limited by the inherent time structure of current X-ray sources, which will be overcome with the introduction of ultrafast and high brilliant X-ray sources.

Fischer, Peter

2010-06-01T23:59:59.000Z

266

The data dictionary: A view into the CTBT knowledge base  

Science Conference Proceedings (OSTI)

The data dictionary for the Comprehensive Test Ban Treaty (CTBT) knowledge base provides a comprehensive, current catalog of the projected contents of the knowledge base. It is written from a data definition view of the knowledge base and therefore organizes information in a fashion that allows logical storage within the computer. The data dictionary introduces two organization categories of data: the datatype, which is a broad, high-level category of data, and the dataset, which is a specific instance of a datatype. The knowledge base, and thus the data dictionary, consist of a fixed, relatively small number of datatypes, but new datasets are expected to be added on a regular basis. The data dictionary is a tangible result of the design effort for the knowledge base and is intended to be used by anyone who accesses the knowledge base for any purpose, such as populating the knowledge base with data, or accessing the data for use with automatic data processing (ADP) routines, or browsing through the data for verification purposes. For these two reasons, it is important to discuss the development of the data dictionary as well as to describe its contents to better understand its usefulness; that is the purpose of this paper.

Shepherd, E.R.; Keyser, R.G.; Armstrong, H.M. [and others

1997-08-01T23:59:59.000Z

267

T-643: HP OpenView Storage Data Protector Unspecified Code Execution  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

643: HP OpenView Storage Data Protector Unspecified Code 643: HP OpenView Storage Data Protector Unspecified Code Execution Vulnerability T-643: HP OpenView Storage Data Protector Unspecified Code Execution Vulnerability June 9, 2011 - 3:45pm Addthis PROBLEM: HP OpenView Storage Data Protector Unspecified Code Execution Vulnerability PLATFORM: Versions 6.0, 6.10, and 6.11 running on HP-UX, Solaris, Linux and Windows. ABSTRACT: A vulnerability has been reported in HP OpenView Storage Data Protector, which can be exploited by malicious people to compromise a vulnerable system. reference LINKS: Secunia Advisory SA44884 CVE-2011-1864 SecurityTracker Alert ID: 1025620 HP Document ID: c02712867 IMPACT ASSESSMENT: High Discussion: A vulnerability was reported in HP OpenView Storage Data Protector. A remote user can execute arbitrary code on the target system.

268

U-025: HP OpenView Network Node Manager Bugs Let Remote Users Execute  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

U-025: HP OpenView Network Node Manager Bugs Let Remote Users U-025: HP OpenView Network Node Manager Bugs Let Remote Users Execute Arbitrary Code U-025: HP OpenView Network Node Manager Bugs Let Remote Users Execute Arbitrary Code November 2, 2011 - 8:00am Addthis PROBLEM: HP OpenView Network Node Manager Bugs Let Remote Users Execute Arbitrary Code. PLATFORM: HP OpenView Network Node Manager (OV NNM) v7.51, v7.53 running on HP-UX, Linux, Solaris, and Windows ABSTRACT: A remote user can execute arbitrary code on the target system. reference LINKS: HP Support Center Document ID: c03054052 SecurityTracker Alert ID: 1026260 CVE-2011-1365 CVE-2011-1366 CVE-2011-1367 IMPACT ASSESSMENT: Medium Discussion: Several vulnerabilities were reported in HP OpenView Network Node Manager. A remote user can execute arbitrary code on the target system.

269

Microsoft Word - A Systems View of the Modern Grid_Final_v2_0.doc  

NLE Websites -- All DOE Office Websites (Extended Search)

V2.0 V2.0 The NETL Modern Grid Initiative A SYSTEMS VIEW OF THE MODERN GRID Conducted by the National Energy Technology Laboratory for the U.S. Department of Energy Office of Electricity Delivery and Energy Reliability January 2007 Office of Electricity Delivery and Energy Reliability Page 1 v2.0 A Systems View of the Modern Grid TABLE OF CONTENTS Table of Contents ..................................................................................... 1 Executive Summary .................................................................................. 2 Creating the Systems View........................................................................ 4 Key Success Factors .................................................................................

270

Enhanced polymeric nanoparticles for gene delivery  

E-Print Network (OSTI)

The potential of gene therapy to treat disease and improve human health is tremendous. The failure of viral gene therapy clinical trials due to toxicity, immunogenicity, and carcinogenicity has been tragic and strongly ...

Green, Jordan Jamieson

2007-01-01T23:59:59.000Z

271

Mysteries of the Deep: What happens inside of MPI on Blue Gene/Q and why it matters  

NLE Websites -- All DOE Office Websites (Extended Search)

Mysteries Mysteries of the Deep: What happens inside of MPI on Blue Gene/Q and why it matters Jeff Hammond Leadership Computing Facility Argonne National Laboratory March 5, 2013 Jeff Hammond PAMI and MPI on BGQ The view from the boat Jeff Hammond PAMI and MPI on BGQ A reason to dive Jeff Hammond PAMI and MPI on BGQ But not too deep Jeff Hammond PAMI and MPI on BGQ Blue Gene/P Communication architecture Jeff Hammond PAMI and MPI on BGQ Blue Gene/Q Communication architecture Jeff Hammond PAMI and MPI on BGQ MPI-3 on Blue Gene/Q There is no official plan to support MPI-3 on Blue Gene/Q but a subset of these features may be available at some point in the future. I have build MPICH-3.0.x on Blue Gene/Q and many things pass while others are completely broken, but this is not a supported usage and you should not do it unless you are l33t h4xz0r. Jeff Hammond PAMI and MPI on BGQ MPI-3 on Blue Gene/Q A portable implementation

272

Concept development for the ITER equatorial port visible/infrared wide angle viewing system  

Science Conference Proceedings (OSTI)

The ITER equatorial port visible/infrared wide angle viewing system concept is developed from the measurement requirements. The proposed solution situates 4 viewing systems in the equatorial ports 3, 9, 12, and 17 with 4 views each (looking at the upper target, the inner divertor, and tangentially left and right). This gives sufficient coverage. The spatial resolution of the divertor system is 2 times higher than the other views. For compensation of vacuum-vessel movements, an optical hinge concept is proposed. Compactness and low neutron streaming is achieved by orienting port plug doglegs horizontally. Calibration methods, risks, and R and D topics are outlined.

Reichle, R.; Beaumont, B.; Boilson, D.; Bouhamou, R.; Direz, M.-F.; Encheva, A.; Henderson, M.; Kazarian, F.; Lamalle, Ph.; Lisgo, S.; Mitteau, R.; Patel, K. M.; Pitcher, C. S.; Pitts, R. A.; Prakash, A.; Raffray, R.; Schunke, B.; Snipes, J.; Diaz, A. Suarez; Udintsev, V. S. [ITER Organization, Route de Vinon-sur-Verdon, 13115 St Paul-lez-Durance (France); and others

2012-10-15T23:59:59.000Z

273

for viewPP version1.00 klic klitcl.kl1  

E-Print Network (OSTI)

PP ( ) 2 #12;viewPP 3 viewpp.tcl GUI Tcl/Tk viewpp viewPP klic klitcl.kl1 viewpp viewPP klitcl 2 klitcld Tcl/Tk klic klitcl Tcl/Tk klic (Tk ) C viewpp klitcld 2 3 viewPP klic (version 2.002) Tcl (version 7.5) Tk (version 4.1) #12;1. / Tcl/Tk ( Tk 3.6 ) 2. OS SunOS 5.4/5.5.1 OS 3. klic I/O (Configure "Does

Tanaka, Jiro

274

A Framework for viewing theoretical, technological, economic and market potential of carbon dioxide capture and storage  

SciTech Connect

Paper presents an intelectual framework for viewing how the theoretical, technological, economic and market potentials of carbon dioxide capture and storage are related to each other.

Dooley, James J.

2004-10-04T23:59:59.000Z

275

Uses of antimicrobial genes from microbial genome  

DOE Patents (OSTI)

We describe a method for mining microbial genomes to discover antimicrobial genes and proteins having broad spectrum of activity. Also described are antimicrobial genes and their expression products from various microbial genomes that were found using this method. The products of such genes can be used as antimicrobial agents or as tools for molecular biology.

Sorek, Rotem; Rubin, Edward M.

2013-08-20T23:59:59.000Z

276

The Harold Brown view: LLNL then and now  

Science Conference Proceedings (OSTI)

Harold Brown was the Laboratory's third Director, serving from 1960 to 1961. He joined the Livermore laboratory in 1952. At Livermore, Brown worked on the Polaris warhead and on Project Plowshare, program designed to apply nuclear explosives to peaceful uses (such as excavating harbors). Brown succeeded Edward Teller as Director of the Livermore Laboratory in July 1960. His tenure as Director was particularly challenging as these were the years of the moratorium on nuclear testing. He was the driving force in expanding the Laboratory's capabilities for simulating nuclear explosions with computers. As part of LLNL's 40th anniversary observances, Brown was invited to lecture on his views of the changing world and the role of the Laboratory. He reminisced about events that occurred in the Laboratory's early years, with an eye to finding lessons for the future. In particular, he cited Project Plowshare and the MX ICBM as examples of projects that were technologically and economically feasible but unacceptable in terms of public perception. Brown also discussed the international security environment and the Laboratory's role in support of the national security goals of the United States. He defined U.S. security as protecting America against external threats to its physical survival, to its democratic form of government, or to the well-being of the people of the United States. By this definition, issues of international trade and market access have a strong bearing on national security. Thus the Laboratory can find much important and interesting work to do under the heading of national security and economic competitiveness. Brown also pointed out, however, that working effectively with the private sector will take a change in culture since the private-sector market is very different from and more competitive than the nuclear weapons world or the government's nondefense market.

Brown, H. (Center for Strategic and International Studies, Washington, DC (United States))

1993-08-01T23:59:59.000Z

277

Method for determining gene knockouts  

DOE Patents (OSTI)

A method for determining candidates for gene deletions and additions using a model of a metabolic network associated with an organism, the model includes a plurality of metabolic reactions defining metabolite relationships, the method includes selecting a bioengineering objective for the organism, selecting at least one cellular objective, forming an optimization problem that couples the at least one cellular objective with the bioengineering objective, and solving the optimization problem to yield at least one candidate.

Maranas, Costas D. (Port Matilda, PA); Burgard, Anthony R. (State College, PA); Pharkya, Priti (State College, PA)

2011-09-27T23:59:59.000Z

278

Content-aware bit allocation in scalable multi-view video coding  

Science Conference Proceedings (OSTI)

We propose a new scalable multi-view video coding (SMVC) method with content-aware bit allocation among multiple views. The video is encoded off-line with a predetermined number of temporal and SNR scalability layers. Content-aware bit allocation among ...

Nükhet Özbek; A. Murat Tekalp

2006-09-01T23:59:59.000Z

279

Probabilistic subspace-based learning of shape dynamics modes for multi-view action recognition  

E-Print Network (OSTI)

-temporal interest point de- scriptors (STIP) [12] for multi-view action recognition. 5.1. Dataset The MuHAVi dataset outperforms [13] which uses Spatio-Temporal Interest Points (STIP) [12] for multi-view action recognition. This method constructs BoW of STIP features for every action. These BoWs are classified using Support Vector

California at Santa Barbara, University of

280

Estimating Probabilities of Cloud-Free Fields-of-View From the Earth Through the Atmosphere  

Science Conference Proceedings (OSTI)

A model for estimating the probability of obtaining a cloud-free field-of-view as a function of ground-observer-reported total sky cover is presented for earth-to-space viewing applications. The development of the model and examples of its ...

Iver A. Lund; Donald D. Grantham; Richard E. Davis

1980-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

The Determination of Sky View-Factors in Urban Environments Using Video Imagery  

Science Conference Proceedings (OSTI)

A technique is described whereby sky view-factors may be determined using a video camera equipped with a fish-eye lens. The video image is digitized and then analyzed to distinguish between “sky” and “non-sky” pixels. View-factors are calculated ...

D. G. Steyn; J. E. Hay; Ian D. Watson; Glenn T. Johnson

1986-12-01T23:59:59.000Z

282

A Multiple View Approach to Support Common Ground in Distributed and Synchronous Geo-Collaboration  

Science Conference Proceedings (OSTI)

In this paper we investigate strategies to support knowledgesharing in distributed, synchronous collaboration. Our goalis to propose, justify, and assess a multiple view approachto support common ground in geo-collaboration within multi-roleteams. We ... Keywords: Multiple views, common ground, distributed and synchronous geo-collaboration

Gregorio Convertino; Craig H. Ganoe; Wendy A. Schafer; Beth Yost; John M. Carroll

2005-07-01T23:59:59.000Z

283

Development of software for spectral imaging data acquisition using LabVIEW  

Science Conference Proceedings (OSTI)

Developing data acquisition software is a major challenge in integrating a spectral imaging system. This paper presents the design and implementation of a data acquisition program using LabVIEW for a liquid crystal tunable filter based spectral imaging ... Keywords: Data acquisition, Finite state machine, Hyperspectral imaging, LCTF, LabVIEW, Software

Weilin Wang; Changying Li; Ernest W. Tollner; Glen C. Rains

2012-06-01T23:59:59.000Z

284

Multi-view Hockey Tracking with Trajectory Smoothing and Camera Selection  

E-Print Network (OSTI)

We address the problem of multi-view multi-target tracking using multiple stationary cameras in the application of hockey tracking and test the approach with data from two cameras. The system is based on the previous work by Okuma et al. [50]. We replace AdaBoost detection with blob detection in both image coordinate systems after background subtraction. The sets of blob-detection results are then mapped to the rink coordinate system using a homography transformation. These observations are further merged into the final detection result which will be incorporated into the particle filter. In addition, we extend the particle filter to use multiple observation models, each corresponding to a view. An observation likelihood and a reference color model are also maintained for each player in each view and are updated only when the player is not occluded in that view. As a result of the expanded coverage range and multiple perspectives in the multi-view tracking, even when the target is occluded in one view, it still can be tracked as long as it is visible from another view. The multi-view tracking data are further processed by trajectory smoothing using the Maximum a posteriori smoother. Finally, automatic camera selection is performed using the Hidden Markov Model to create personalized video programs. ii Table of Contents Abstract................................. ii

Lan Wu

2008-01-01T23:59:59.000Z

285

MobiUS: enable together-viewing video experience across two mobile devices  

Science Conference Proceedings (OSTI)

We envision a new better-together mobile application paradigm where multiple mobile devices are placed in a close proximity and study a specific together-viewing video application in which a higher resolution video isplayed back across ... Keywords: better-together, collaborative decoding, energy efficiency, mobile computing, proximity detection, resource aggregation, screen aggregation, together-viewing

Guobin Shen; Yanlin Li; Yongguang Zhang

2007-06-01T23:59:59.000Z

286

Hybrid clustering of multi-view data via Tucker-2 model and its application  

Science Conference Proceedings (OSTI)

With the modern technology fast developing, most of entities can be observed by different perspectives. These multiple view information allows us to find a better pattern as long as we integrate them in an appropriate way. So clustering by integrating ... Keywords: Bibliometric analysis, Hybrid clustering, Multi-view data, Text mining

Xinhai Liu; Wolfgang Glänzel; Bart De Moor

2011-09-01T23:59:59.000Z

287

Recombinant HT{sub m4} gene, protein and assays  

DOE Patents (OSTI)

The invention relates to a recombinant DNA molecule which encodes a HT{sub m4} protein, a transformed host cell which has been stably transfected with a DNA molecule which encodes a HT{sub m4} protein and a recombinant HT{sub m4} protein. The invention also relates to a method for detecting the presence of a hereditary atopy. 2 figs.

Lim, B.; Adra, C.N.; Lelias, J.M.

1996-09-03T23:59:59.000Z

288

Prairie View A&M University | Princeton Plasma Physics Lab  

NLE Websites -- All DOE Office Websites (Extended Search)

Prairie View A&M University Prairie View A&M University Prairie View A&M University, Historically Black College and University (HBCU) Professor Tian-sen Huang The Prairie View Plasma Lab (PVPL) is the only fusion plasma lab operated at an HBCU, and a major research and education laboratory at Prairie View A&M University (PVAMU). The lab has two rotamak devices supported by two 500kHz/400 kW rf generators to operate a rotating magnetic field drive-FRC plasma. Since PVPL 2001, PPPL/OSUR provided significant technical and material support during the lab's construction, and assisted in the installation and initial operation of the rotomak FRC plasma device. Because there is no engineering support service at PVAMU, PPPL organized an engineering team that gave the PVPL team assistance in assembling the

289

T-704: RSA enVision Lets Remote Users View Files and Remote Authenticated  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

704: RSA enVision Lets Remote Users View Files and Remote 704: RSA enVision Lets Remote Users View Files and Remote Authenticated Users Obtain Password T-704: RSA enVision Lets Remote Users View Files and Remote Authenticated Users Obtain Password August 29, 2011 - 3:45am Addthis PROBLEM: Allows remote attackers to obtain sensitive information by sniffing the network or leveraging access to a recipient mailbox. PLATFORM: RSA enVision Version(s): 3.x, 4.x ABSTRACT: RSA enVision lets remote users view files and remote authenticated users obtain password. reference LINKS: SecurityTracker Alert ID: 1025979 CVE-2011-2736 CVE-2011-2737 RSA enVision Product Security IMPACT ASSESSMENT: Medium Discussion: Two vulnerabilities were reported in RSA enVision. A remote user can view files on the target system. A remote authenticated user can obtain

290

T-704: RSA enVision Lets Remote Users View Files and Remote Authenticated  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

4: RSA enVision Lets Remote Users View Files and Remote 4: RSA enVision Lets Remote Users View Files and Remote Authenticated Users Obtain Password T-704: RSA enVision Lets Remote Users View Files and Remote Authenticated Users Obtain Password August 29, 2011 - 3:45am Addthis PROBLEM: Allows remote attackers to obtain sensitive information by sniffing the network or leveraging access to a recipient mailbox. PLATFORM: RSA enVision Version(s): 3.x, 4.x ABSTRACT: RSA enVision lets remote users view files and remote authenticated users obtain password. reference LINKS: SecurityTracker Alert ID: 1025979 CVE-2011-2736 CVE-2011-2737 RSA enVision Product Security IMPACT ASSESSMENT: Medium Discussion: Two vulnerabilities were reported in RSA enVision. A remote user can view files on the target system. A remote authenticated user can obtain

291

Human gene sequencing makes advances  

SciTech Connect

The Human Genome Project is a federal project that is on the scale of the Manhattan Project of the 1940s. The focus of this project is to map and sequence the 100,000 plus genes and 3 billion base pairs that comprise the human genome. This effort has made two recent advances. First, two of the major companies involved in this project formed a strategic alliance that will pump up to 125 million dollars into this project. Second, researchers at Argonne National Lab. have tested a new sequencing technique that could identify 100 million base pairs a day when fully implemented.

Alper, J.

1993-09-01T23:59:59.000Z

292

QB1 - Stochastic Gene Regulation  

Science Conference Proceedings (OSTI)

Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present in the cell - Random motion and competition between reactants, Low copy, quantization of reactants, Upstream processes; (2) Fluctuations may be very important - Cell-to-cell variability, Cell fate decisions (switches), Signal amplification or damping, stochastic resonances; and (3) Some tools are available to mode these - Kinetic Monte Carlo simulations (SSA and variants), Moment approximation methods, Finite State Projection. We will see how modeling these reactions can tell us more about the underlying processes of gene regulation.

Munsky, Brian [Los Alamos National Laboratory

2012-07-23T23:59:59.000Z

293

Imaging gene expression in real-time using aptamers  

Science Conference Proceedings (OSTI)

Signal transduction pathways are usually activated by external stimuli and are transient. The downstream changes such as transcription of the activated genes are also transient. Real-time detection of promoter activity is useful for understanding changes in gene expression, especially during cell differentiation and in development. A simple and reliable method for viewing gene expression in real time is not yet available. Reporter proteins such as fluorescent proteins and luciferase allow for non-invasive detection of the products of gene expression in living cells. However, current reporter systems do not provide for real-time imaging of promoter activity in living cells. This is because of the long time period after transcription required for fluorescent protein synthesis and maturation. We have developed an RNA reporter system for imaging in real-time to detect changes in promoter activity as they occur. The RNA reporter uses strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags), which can be expressed from a promoter of choice. The tobramycin, neomycin and PDC RNA aptamers have been utilized for this system and expressed in yeast from the GAL1 promoter. The IMAGEtag RNA kinetics were quantified by RT-qPCR. In yeast precultured in raffinose containing media the GAL1 promoter responded faster than in yeast precultured in glucose containing media. IMAGEtag RNA has relatively short half-life (5.5 min) in yeast. For imaging, the yeast cells are incubated with their ligands that are labeled with fluorescent dyes. To increase signal to noise, ligands have been separately conjugated with the FRET (Förster resonance energy transfer) pairs, Cy3 and Cy5. With these constructs, the transcribed aptamers can be imaged after activation of the promoter by galactose. FRET was confirmed with three different approaches, which were sensitized emission, acceptor photobleaching and donor lifetime by FLIM (fluorescence lifetime imaging microscopy). Real-time transcription was measured by FLIM-FRET, which was detected by the decrease in donor lifetime resulting from ligand binding to IMAGEtags that were newly synthesized from the activated GAL1 promoter. The FRET signal was specific for transcribed IMAGEtags.

Shin, Il Chung

2011-12-13T23:59:59.000Z

294

Methods for monitoring multiple gene expression  

DOE Patents (OSTI)

The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

Berka, Randy; Bachkirova, Elena; Rey, Michael

2013-10-01T23:59:59.000Z

295

Methods for monitoring multiple gene expression  

DOE Patents (OSTI)

The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

Berka, Randy (Davis, CA); Bachkirova, Elena (Davis, CA); Rey, Michael (Davis, CA)

2008-06-01T23:59:59.000Z

296

Nano-vesicles for gene delivery.  

E-Print Network (OSTI)

??Gene therapy was considered an important modality for the potential treatment of cancer. It aimed to correct diseases through the delivery of genetic material encoding… (more)

Tan, Karen Li Ping.

2008-01-01T23:59:59.000Z

297

Bayesian survival analysis using gene expression.  

E-Print Network (OSTI)

??This thesis developed and applied Bayesian models for the analysis of survival data. The gene expression was considered as explanatory variables within the Bayesian survival… (more)

Thamrin, Sri Astuti

2013-01-01T23:59:59.000Z

298

U-008: Symantec Data Loss Prevention Bugs in KeyView Filter Lets Remote  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

08: Symantec Data Loss Prevention Bugs in KeyView Filter Lets 08: Symantec Data Loss Prevention Bugs in KeyView Filter Lets Remote Users Deny Service U-008: Symantec Data Loss Prevention Bugs in KeyView Filter Lets Remote Users Deny Service October 11, 2011 - 8:00am Addthis PROBLEM: Symantec Data Loss Prevention Bugs in KeyView Filter Lets Remote Users Deny Service PLATFORM: Symantec Data Loss Prevention Enforce/Detection Servers for Windows 10.x, 11.x ABSTRACT: A remote user can create a file that, when processed by the target filter, will cause partial denial of service conditions. reference LINKS: Symantec Security Advisory SYM11-013 SecurityTracker Alert ID: 1026157 IMPACT ASSESSMENT: Medium Discussion: Multiple vulnerabilities were reported in Symantec Data Loss Prevention. A remote user can cause denial of service conditions on the target system.A

299

ParaView Red Blood Cell Tutorial on Eureka/Gadzooks | Argonne Leadership  

NLE Websites -- All DOE Office Websites (Extended Search)

Red Blood Cell Tutorial on Eureka/Gadzooks Red Blood Cell Tutorial on Eureka/Gadzooks Goals This tutorial is intended to be a hands-on resource for users interested in learning the basic concepts of ParaView. The examples can easily be run on a laptop, using the example data set provided. Tour of ParaView Show range of visualization methods Walk through various visualization techniques, hopefully illustrate how these can apply to your own data. Feel for ParaView "way" Terminology and step-by-step process peculiar to ParaView, which may differ from other packages, e.g. VisIt. cutting planes: close-up RBCs: close-up RBC: Continuum Data The data used for this tutorial is: Blood flow simulation data Multiple data types Continuum data field (unstructured mesh, tetrahedral): fluid field, plasma

300

DOE Solicits Views on the Implementation of Large-Capacity Clothes Washer  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Solicits Views on the Implementation of Large-Capacity Clothes Solicits Views on the Implementation of Large-Capacity Clothes Washer Waivers DOE Solicits Views on the Implementation of Large-Capacity Clothes Washer Waivers November 30, 2010 - 10:56am Addthis The Department of Energy has recently granted several requests for waivers establishing an alternative test procedure for certain large-capacity residential clothes washer models. We have now received follow-up questions about the proper application of these waivers. Since these questions affect multiple manufacturers and the public, we have decided the proper course is to invite interested parties to submit views on the proper application of these waivers to existing clothes washer models and units by Tuesday, December 7th. The Department's current test procedure for residential clothes washers

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

NREL: Awards and Honors - PowerView Semi-Transparent Photovoltaic Module  

NLE Websites -- All DOE Office Websites (Extended Search)

PowerView(tm) Semi-Transparent Photovoltaic Module PowerView(tm) Semi-Transparent Photovoltaic Module Developers: Harrin Ullal, Ken Zweibel, and Bolko von Roedern, National Renewable Energy Laboratory; Robert S. Oswald and Frank Liu, BP Solar The PowerView(tm) module - a BP Solar commercial product - represents the coming of a new era in photovoltaics for buildings. Because it is semi-transparent, it can be used in lieu of architectural glass for many applications, particularly for those that call for sloped glazing, such as awnings, canopies, or slanted roofs. And because it is photovoltaics, the module uses sunlight to generate clean electricity to power a building's electrical needs. As testimony to the PowerView's utility BP Solar has already installed it on canopies of hundreds of its Connect stores -

302

View the Apps for Vehicles Phase One Submissions | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

View the Apps for Vehicles Phase One Submissions View the Apps for Vehicles Phase One Submissions View the Apps for Vehicles Phase One Submissions January 24, 2013 - 11:13am Addthis 9 of the 37 entries for the Apps for Vehicles competition. 9 of the 37 entries for the Apps for Vehicles competition. Ian Kalin Director of the Energy Data Initiative How can I participate? Visit the competition website to view the submitted ideas. Starting on March 15, you'll be able to vote for your favorite completed apps. Apps for Vehicles The Open Data Initiative reached another milestone last week with the completion of the first phase of the Apps for Vehicles Challenge. This incentive-based competition seeks to improve safety and fuel efficiency through data innovation. Thirty-seven entries were submitted to the Challenge's judges, which are all publically viewable on the competition

303

The Structure of the Unstable Marine Boundary Layer Viewed by Lidar and Aircraft Observations  

Science Conference Proceedings (OSTI)

The combination of vertical lidar and in situ meteorological observations from two aircraft provide an unprecedented view of the marine atmospheric boundary layer (MABL) during a cold air outbreak. To a first approximation, the lidar reflectivity ...

David Atlas; Bernard Walter; Shu-Hsien Chou; P. J. Sheu

1986-07-01T23:59:59.000Z

304

Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices  

E-Print Network (OSTI)

impacts of wind energy facilities on the sales prices ofprices were affected by views of and proximity to wind energyprices, and locations in electronic form from local assessors; and (3) the representativeness of the types of wind energy

Hoen, Ben

2012-01-01T23:59:59.000Z

305

A research study investigating methods of obtaining the views and opinions of disabled children about spirituality.  

E-Print Network (OSTI)

??Aims: To identify and evaluate effective interview methods for accessing disabled children’s views on spirituality. To explore disabled children’s theories of spirituality with regards to… (more)

Loader, Beth

2010-01-01T23:59:59.000Z

306

A Mean Synoptic View of the Subantarctic Front South of Australia  

Science Conference Proceedings (OSTI)

A mean synoptic view of the subantarctic front (SAF) is obtained from current meter and hydrographic data by averaging absolute and baroclinic velocity measurements in bins defined by a cross-stream coordinate that moves with the current. The ...

Helen E. Phillips; Stephen R. Rintoul

2002-05-01T23:59:59.000Z

307

Imperial Sea View Hot Springs Pool & Spa Low Temperature Geothermal  

Open Energy Info (EERE)

Sea View Hot Springs Pool & Spa Low Temperature Geothermal Sea View Hot Springs Pool & Spa Low Temperature Geothermal Facility Jump to: navigation, search Name Imperial Sea View Hot Springs Pool & Spa Low Temperature Geothermal Facility Facility Imperial Sea View Hot Springs Sector Geothermal energy Type Pool and Spa Location Niland, California Coordinates 33.2400366°, -115.5188756° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[]}

308

Microsoft Word - A Systems View of the Modern Grid_Final_v2_0...  

NLE Websites -- All DOE Office Websites (Extended Search)

it modern, or smart, or intelligent, but by first building the construct of a modern grid that serves a defined purpose. The systems view approach was used by the NETL MGI team...

309

V-195: RSA Authentication Manager Lets Local Users View the Administrative  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

95: RSA Authentication Manager Lets Local Users View the 95: RSA Authentication Manager Lets Local Users View the Administrative Account Password V-195: RSA Authentication Manager Lets Local Users View the Administrative Account Password July 9, 2013 - 12:51am Addthis PROBLEM: RSA Authentication Manager Lets Local Users View the Administrative Account Password PLATFORM: RSA Authentication Manager 7.1, 8.0 ABSTRACT: A vulnerability was reported in RSA Authentication Manager. REFERENCE LINKS: SecurityTracker Alert ID: 1028742 CVE-2013-3273 RSA IMPACT ASSESSMENT: Medium DISCUSSION: When the RSA Authentication Manager Software Development Kit (SDK) is used to develop a custom application that connects with RSA Authentication Manager and the trace logging is set to verbose, the administrative account password used by the custom application is written in clear text to trace

310

Gene therapy in alcoholic rats  

NLE Websites -- All DOE Office Websites (Extended Search)

70 70 Sept. 9, 2001 Gene Therapy Reduces Drinking in "Alcoholic" Rats UPTON, NY - Scientists at the U.S. Department of Energy's Brookhaven National Laboratory have shown that increasing the level of a brain protein important for transmitting pleasure signals can turn rats that prefer alcohol into light drinkers, and those with no preference into near teetotalers. The findings, published in the first September 2001 issue of the Journal of Neurochemistry (Vol. 78, No. 5), may have implications for the prevention and treatment of alcoholism in humans. "This is a preliminary study, but when you see a rat that chooses to drink 80 to 90 percent of its daily fluid as alcohol, and then three days later it's down to 20 percent, that's a dramatic drop in alcohol intake - a very clear change in behavior," said Panayotis Thanos, the lead researcher. "This gives us great hope that we can refine this treatment for future clinical use."

311

Microarray gene expression classification with few genes: Criteria to combine attribute selection and classification methods  

Science Conference Proceedings (OSTI)

Microarray data classification is a task involving high dimensionality and small samples sizes. A common criterion to decide on the number of selected genes is maximizing the accuracy, which risks overfitting and usually selects more genes than actually ... Keywords: Efficient classification with few genes, Feature selection, Machine learning, Microarray data classification

Carlos J. Alonso-González; Q. Isaac Moro-Sancho; Arancha Simon-Hurtado; Ricardo Varela-Arrabal

2012-06-01T23:59:59.000Z

312

PlantView OMX Web Services, Version 1.4 User's Manual  

Science Conference Proceedings (OSTI)

PlantView174 is an enterprise asset management system designed specifically for power generation applications that require system-wide access to information on equipment operational and health status. PlantView174 is a modular tool installed on an enterprise server with access by operations, maintenance, and system owners. Modules are devoted to specific functions such as maintenance basis development; predictive maintenance tech exam results; system health; and operators shift logs. Software enhancemen...

2011-12-15T23:59:59.000Z

313

The Impact of YouTube Recommendation System on Video Views  

E-Print Network (OSTI)

Hosting a collection of millions of videos, YouTube offers several features to help users discover the videos of their interest. For example, YouTube provides video search, related video recommendation and front page highlight. The understanding of how these features drive video views is useful for creating a strategy to drive video popularity. In this paper, we perform a measurement study on data sets crawled from YouTube and find that the related video recommendation, which recommends the videos that are related to the video a user is watching, is one of the most important view sources of videos. Despite the fact that the YouTube video search is the number one source of views in aggregation, the related video recommendation is the main source of views for the majority of the videos on YouTube. Furthermore, our results reveal that there is a strong correlation between the view count of a video and the average view count of its top referrer videos. This implies that a video has a higher chance to become popular when it is placed on the related video recommendation lists of popular videos. We also find that the click through rate from a video to its related videos is high and the position of a video in a related video list plays a critical role in the click through rate. Finally, our evaluation of the impact of the related video recommendation system on the diversity of video views indicates that the current recommendation system helps to increase the diversity of video views in aggregation.

Renjie Zhou; Samamon Khemmarat; Lixin Gao

2010-01-01T23:59:59.000Z

314

PlantView Engineering Analysis Module 2.119.001, User's Manual  

Science Conference Proceedings (OSTI)

The PlantViewEngineering Analysis Module is used to compare numerical results for similar equipment types across units, plants or departments. Using the flexibility of the web to seamlessly integrate information from multiple sources, users define Data Points to query ODBC databases for information. On a periodic basis the current value for the data point is obtained. Alarm & Data Quality processing is performed before the information is stored in PlantViewIf real-time values are not available, manual en...

2011-10-04T23:59:59.000Z

315

Graph theoretic approach to parallel gene assembly  

Science Conference Proceedings (OSTI)

We study parallel complexity of signed graphs motivated by the highly complex genetic recombination processes in ciliates. The molecular gene assembly operations have been modeled by operations of signed graphs, i.e., graphs where the vertices have a ... Keywords: Double-split graphs, Gene assembly, Local complement, Parallel assembly, Perfect matching, Signed graphs, Split graphs

Tero Harju; Chang Li; Ion Petre

2008-11-01T23:59:59.000Z

316

Scalable Time Warp on Blue Gene Supercomputers  

Science Conference Proceedings (OSTI)

n this paper we illustrate scalable parallel performance for the TimeWarp synchronization protocol on the L and P variants of the IBM BlueGene supercomputer. Scalable Time Warp performance for models that communicate a large percentage of the event population ... Keywords: Time Warp, Blue Gene Supercomputer

David W. Bauer Jr.; Christopher D. Carothers; Akintayo Holder

2009-06-01T23:59:59.000Z

317

Noise minimization in eukaryotic gene expression  

Science Conference Proceedings (OSTI)

All organisms have elaborate mechanisms to control rates of protein production. However, protein production is also subject to stochastic fluctuations, or noise. Several recent studies in Saccharomyces cerevisiae and Escherichia coli have investigated the relationship between transcription and translation rates and stochastic fluctuations in protein levels, or more generally, how such randomness is a function of intrinsic and extrinsic factors. However, the fundamental question of whether stochasticity in protein expression is generally biologically relevant has not been addressed, and it remains unknown whether random noise in the protein production rate of most genes significantly affects the fitness of any organism. We propose that organisms should be particularly sensitive to variation in the protein levels of two classes of genes: genes whose deletion is lethal to the organism and genes that encode subunits of multiprotein complexes. Using an experimentally verified model of stochastic gene expression in S. cerevisiae, we estimate the noise in protein production for nearly every yeast gene, and confirm our prediction that the production of essential and complex-forming proteins involves lower levels of noise than does the production of most other genes. Our results support the hypothesis that noise in gene expression is a biologically important variable, is generally detrimental to organismal fitness, and is subject to natural selection.

Fraser, Hunter B.; Hirsh, Aaron E.; Giaever, Guri; Kumm, Jochen; Eisen, Michael B.

2004-01-15T23:59:59.000Z

318

Gene coding for the E1 endoglucanase  

DOE Patents (OSTI)

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol. 6 figs.

Thomas, S.R.; Laymon, R.A.; Himmel, M.E.

1996-07-16T23:59:59.000Z

319

Induction and selection of the most interesting Gene Ontology based multiattribute rules for descriptions of gene groups  

Science Conference Proceedings (OSTI)

A rules induction algorithm dedicated to describe groups of genes with similar expression profiles by means of Gene Ontology terms is discussed in the paper. The presented algorithm takes into consideration information contained in the Gene Ontology ... Keywords: Decision rules, Gene Ontology, Gene groups descriptions, Rough sets, Rule interestingness measures

Marek Sikora; Aleksandra Gruca

2011-01-01T23:59:59.000Z

320

Tissue-Specific Gene Delivery via Nanoparticle Coating  

E-Print Network (OSTI)

The use of biomaterials for gene delivery can potentially avoid many of the safety concerns with viral gene delivery. However, the efficacy of polymeric gene delivery methods is low, particularly in vivo. One significant ...

Harris, Todd J.

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Science at LLNL with IBM Blue Gene/Q  

Science Conference Proceedings (OSTI)

Lawrence Livermore National Laboratory (LLNL) has a long history of working with IBM on Blue Gene® supercomputers. Beginning in November 2001 with the joint announcement of a partnership to expand the Blue Gene research project (including Blue Gene®/L ...

B. Carnes, B. Chan, E. W. Draeger, J.-L. Fattebert, L. Fried, J. Glosli, W. D. Krauss, S. H. Langer, R. McCallen, A. A. Mirin, F. Najjar, A. L. Nichols, T. Oppelstrup, J. A. Rathkopf, D. Richards, F. Streitz, P. M. Vranas, J. J. Rice, J. A. Gunnels, V. Gurev, C. Kim, J. Magerlein, M. Reumann, H.-F. Wen

2013-01-01T23:59:59.000Z

322

Identification of candidate genes in Arabidopsis and Populus...  

NLE Websites -- All DOE Office Websites (Extended Search)

in cell wall biosynthesis or remodeling. The 121 genes were then used as bait genes to query an Arabidopsis co-expression database, and additional genes were identified as...

323

Radiation-induced gene responses  

SciTech Connect

In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

1996-12-31T23:59:59.000Z

324

Visualizing Gene Expression In Situ  

Science Conference Proceedings (OSTI)

Visualizing bacterial cells and describing their responses to the environment are difficult tasks. Their small size is the chief reason for the difficulty, which means that we must often use many millions of cells in a sample in order to determine what the average response of the bacteria is. However, an average response can sometimes mask important events in bacterial physiology, which means that our understanding of these organisms will suffer. We have used a variety of instruments to visualize bacterial cells, all of which tell us something different about the sample. We use a fluorescence activated cell sorter to sort cells based on the fluorescence provided by bioreporter genes, and these can be used to select for particular genetic mutations. Cells can be visualized by epifluorescent microscopy, and sensitive photodetectors can be added that allow us to find a single bacterial cell that is fluorescent or bioluminescent. We have also used standard photomultipliers to examine cell aggregates as field bioreporter microorganisms. Examples of each of these instruments show how our understanding of bacterial physiology has changed with the technology.

Burlage, R.S.

1998-11-02T23:59:59.000Z

325

Algorithms for Gene Clustering Analysis on Genomes  

E-Print Network (OSTI)

The increased availability of data in biological databases provides many opportunities for understanding biological processes through these data. As recent attention has shifted from sequence analysis to higher-level analysis of genes across multiple genomes, there is a need to develop efficient algorithms for these large-scale applications that can help us understand the functions of genes. The overall objective of my research was to develop improved methods which can automatically assign groups of functionally related genes in large-scale data sets by applying new gene clustering algorithms. Proposed gene clustering algorithms that can help us understand gene function and genome evolution include new algorithms for protein family classification, a window-based strategy for gene clustering on chromosomes, and an exhaustive strategy that allows all clusters of small size to be enumerated. I investigate the problems of gene clustering in multiple genomes, and define gene clustering problems using mathematical methodology and solve the problems by developing efficient and effective algorithms. For protein family classification, I developed two supervised classification algorithms that can assign proteins to existing protein families in public databases and, by taking into account similarities between the unclassified proteins, allows for progressive construction of new families from proteins that cannot be assigned. This approach is useful for rapid assignment of protein sequences from genome sequencing projects to protein families. A comparative analysis of the method to other previously developed methods shows that the algorithm has a higher accuracy rate and lower mis-classification rate when compared to algorithms that are based on the use of multiple sequence alignments and hidden Markov models. The proposed algorithm performs well even on families with very few proteins and on families with low sequence similarity. Apart from the analysis of individual sequences, identifying genomic regions that descended from a common ancestor helps us study gene function and genome evolution. In distantly related genomes, clusters of homologous gene pairs serve as evidence used in function prediction, operon detection, etc. Thus, reliable identification of gene clusters is critical to functional annotation and analysis of genes. I developed an efficient gene clustering algorithm that can be applied on hundreds of genomes at the same time. This approach allows for large-scale study of evolutionary relationships of gene clusters and study of operon formation and destruction. By placing a stricter limit on the maximum cluster size, I developed another algorithm that uses a different formulation based on constraining the overall size of a cluster and statistical estimates that allow direct comparisons of clusters of different size. A comparative analysis of proposed algorithms shows that more biological insight can be obtained by analyzing gene clusters across hundreds of genomes, which can help us understand operon occurrences, gene orientations and gene rearrangements.

Yi, Gang Man

2011-05-01T23:59:59.000Z

326

Single, Key Gene Discovery Could Streamline Production of Biofuels...  

NLE Websites -- All DOE Office Websites (Extended Search)

Single, Key Gene Discovery Could Streamline Production of Biofuels Single, Key Gene Discovery Could Streamline Production of Biofuels August 11, 2011 - 3:51pm Addthis WASHINGTON,...

327

The specificity and evolution of gene regulatory elements  

E-Print Network (OSTI)

The regulation of gene expression underlies the morphological, physiological, and functional differences between human cell types, developmental stages, and healthy and disease states. Gene regulation in eukaryotes is ...

Friedman, Robin Carl

2010-01-01T23:59:59.000Z

328

New Window Technology Saves Energy and the View | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

New Window Technology Saves Energy and the View New Window Technology Saves Energy and the View New Window Technology Saves Energy and the View November 5, 2013 - 3:55pm Addthis Researchers at the Energy Department's National Renewable Energy Laboratory are developing innovative new window technology that helps improve occupants' comfort and cuts energy use. | Photo courtesy of Pat Corkery, NREL. Researchers at the Energy Department's National Renewable Energy Laboratory are developing innovative new window technology that helps improve occupants' comfort and cuts energy use. | Photo courtesy of Pat Corkery, NREL. Gabrial Boeckman NREL Communications Manager for Buildings & Communities What does this project do? With funding from the Energy Department, the National Renewable Energy Laboratory is working to develop innovative insulating window film

329

Transmission Asset Optimization (and) A Contrarian View of the Future of  

NLE Websites -- All DOE Office Websites (Extended Search)

Transmission Asset Optimization (and) A Contrarian View of the Future of Transmission Asset Optimization (and) A Contrarian View of the Future of Distributed Generation vs. Central Station Generation Speaker(s): Dale Bradshaw Date: June 5, 2000 - 10:00am Location: 90-3148 Seminar Host/Point of Contact: David Faulkner This was a two part seminar: Part One: Transmission Asset Optimization Presentation of latest technology developments that can increase the flow of power over todays transmission lines, thus potentially reducing total costs for transmitting power, while improving transmission reliability. Technologies discussed include FACTs devices, energy storage, etc.) Part Two: A Contrarian View of the Future of Distributed Generation vs. Central Station Generation: There have been a lot claims and hype concerning the relative competitive position in the future of distributed generation (DG)

330

Photo of the Week: Satellite View of Sandy at Night | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Photo of the Week: Satellite View of Sandy at Night Photo of the Week: Satellite View of Sandy at Night Photo of the Week: Satellite View of Sandy at Night November 2, 2012 - 10:21am Addthis On Monday, October 29, 2012, Hurricane Sandy made landfall 5 miles south of Atlantic City, New Jersey, with maximum sustained winds near 80 mph. This satellite image was taken 16 to 18 hours before Sandy's landfall on the New Jersey coast, using the Visible Infrared Imaging Radiometer Suite on NASA's Suomi National Polar-orbiting Partnership satellite. The Department of Energy, in partnership with the Federal Emergency Management Administration (FEMA) and other federal agencies, is working around the clock to support the states and utilities that have been impacted by Sandy. Learn more about federal efforts to support utility power restoration. | Photo courtesy of CIMSS/University Wisconsin-Madison/NASA/NOAA.

331

DOE Seeks Public Views On How Best To Streamline Existing Regulations |  

NLE Websites -- All DOE Office Websites (Extended Search)

Public Views On How Best To Streamline Existing Public Views On How Best To Streamline Existing Regulations DOE Seeks Public Views On How Best To Streamline Existing Regulations January 31, 2011 - 5:53pm Addthis The Department of Energy today announced two immediate steps to implement the Obama Administration's Executive Order on Improving Regulation and Regulatory Review, which directs federal agencies to review existing regulations and determine whether they are still necessary and crafted effectively to solve current problems. First, to engage the public in a transparent review process, the Department is issuing a Request for Information asking the public how best to streamline existing regulations and to identify rules that are obsolete, unnecessary, unjustified, or simply no longer make sense. All comments and reply

332

DOE Seeks Public Views On How Best To Streamline Existing Regulations |  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

DOE Seeks Public Views On How Best To Streamline Existing DOE Seeks Public Views On How Best To Streamline Existing Regulations DOE Seeks Public Views On How Best To Streamline Existing Regulations January 31, 2011 - 5:53pm Addthis The Department of Energy today announced two immediate steps to implement the Obama Administration's Executive Order on Improving Regulation and Regulatory Review, which directs federal agencies to review existing regulations and determine whether they are still necessary and crafted effectively to solve current problems. First, to engage the public in a transparent review process, the Department is issuing a Request for Information asking the public how best to streamline existing regulations and to identify rules that are obsolete, unnecessary, unjustified, or simply no longer make sense. All comments and reply

333

New Window Technology Saves Energy and the View | Department of Energy  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

New Window Technology Saves Energy and the View New Window Technology Saves Energy and the View New Window Technology Saves Energy and the View November 5, 2013 - 3:55pm Addthis Researchers at the Energy Department's National Renewable Energy Laboratory are developing innovative new window technology that helps improve occupants' comfort and cuts energy use. | Photo courtesy of Pat Corkery, NREL. Researchers at the Energy Department's National Renewable Energy Laboratory are developing innovative new window technology that helps improve occupants' comfort and cuts energy use. | Photo courtesy of Pat Corkery, NREL. Gabrial Boeckman NREL Communications Manager for Buildings & Communities What does this project do? With funding from the Energy Department, the National Renewable Energy Laboratory is working to develop innovative insulating window film

334

3D View Inside the Skeleton with X-ray Microscopy: Imaging Bone at the  

NLE Websites -- All DOE Office Websites (Extended Search)

3D View Inside the Skeleton with X-ray Microscopy: Imaging Bone 3D View Inside the Skeleton with X-ray Microscopy: Imaging Bone at the Nanoscale Scientists studying osteoporosis and other skeletal diseases are interested in the 3D structure of bone and its responses to conditions such as weightlessness, radiation (of particular interest to astronauts) and vitamin D deficiency. The current gold standard, micro-computed tomography (micro-CT), provides 3D images of trabeculae, the small interior struts of bone tissue, and electron microscopy can provide nanometer resolution of thin tissue slices. Hard X-ray transmission microscopy has provided the first 3D view of bone structure within individual trabeculae on the nanoscale. figure 1 Figure 1 Micro-CT (left) shows trabecular structure inside of bone. Transmission X-ray microscopy (TXM; center and right) can reveal localized details of osteocyte lacunae and their processes.

335

Valley View Hot Springs Pool & Spa Low Temperature Geothermal Facility |  

Open Energy Info (EERE)

Valley View Hot Springs Pool & Spa Low Temperature Geothermal Facility Valley View Hot Springs Pool & Spa Low Temperature Geothermal Facility Facility Valley View Hot Springs Sector Geothermal energy Type Pool and Spa Location Villa Grove, Colorado Coordinates 38.248999°, -105.948608° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[]}

336

The Humboldt House-Rye Patch Geothermal District: An Interim View | Open  

Open Energy Info (EERE)

The Humboldt House-Rye Patch Geothermal District: An Interim View The Humboldt House-Rye Patch Geothermal District: An Interim View Jump to: navigation, search OpenEI Reference LibraryAdd to library Conference Paper: The Humboldt House-Rye Patch Geothermal District: An Interim View Abstract The Humboldt House - Rye Patch Geothermal District extends about 6 miles along the northwestern flank of the Humboldt Range in Pershing County, Nevada and is composed of a number of geothermal cells. The northern Humboldt House portion of the district hosts hot wells and silicic sinter deposits extending from within the Humboldt Range, westward for at least four miles, out into the Humboldt River Valley. The southern Rye Patch portion of the District has scant surface geothermal features, and is identified from well data. Exploration in the District in the mid to late

337

COMPARATIVE ASSESSMENT OF RADIATION-INDUCED GENE  

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with 0, 50 or 200 cGy gamma-rays and the cells harvested for RNA 48 hours post irradiation. The RNA was hybridized to RAE 230A Affymetrix microarrays and differences in gene...

338

Affymetrix White Paper: GeneChip  

E-Print Network (OSTI)

Affymetrix® White Paper: GeneChip® 3' IVT Express Kit May 14, 2009 P/N WH105 Page 1 of 9 White and optimized reagents that enable lower RNA input and increased ease of use. In this white paper, we describe, and the plastic consumables needed to run the assay. #12;Affymetrix® White Paper: GeneChip® 3' IVT Express Kit May

Wandless, Tom

339

Functional Specification: Operations and Maintenance Excellence PlantView Risk Assessment Module Modifications  

Science Conference Proceedings (OSTI)

This report specifies changes to the Risk module of the PlantView software as part of the Electric Power Research Institute's (EPRI's) Operations and Maintenance Excellence (OMX) initiative. The proposed concept for a risk-informed fossil plant maintenance module builds on previous EPRI research and development that has produced applications such as REaP, LP Rim Life, Turbo-X, Boiler-OIO, and the PlantView Risk Assessment module. Risk assessment will then be more effectively integrated with other key pro...

2010-07-28T23:59:59.000Z

340

View and download all Security Smarts for your safety and security meetings  

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View and download all Security Smarts for your safety and security meetings View and download all Security Smarts for your safety and security meetings http://int.lanl.gov/security/documents/index.shtml#security-smarts Note: Security Smarts are current as of the date of publication. SecuritySmart Proof of United States Citizenship for the Badge Office The Badge Office is required by law to verify US citizenship. The following lists the acceptable documents that individuals can bring to the Badge Office. Native-born US Citizens Primary Evidence For an individual born in the United States, a current United States passport or a birth

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341

Mutations of the GREAT gene cause cryptorchidism  

E-Print Network (OSTI)

DDBJ/EMBL/GenBank accession no. AF453828 In humans, failure of testicular descent (cryptorchidism) is one of the most frequent congenital malformations, affecting 1–3 % of newborn boys. The clinical consequences of this abnormality are infertility in adulthood and a significantly increased risk of testicular malignancy. Recently, we described a mouse transgene insertional mutation, crsp, causing high intraabdominal cryptorchidism in homozygous males. A candidate gene Great (G-protein-coupled receptor affecting testis descent), was identified within the transgene integration site. Great encodes a seven-transmembrane receptor with a close similarity to the glycoprotein hormone receptors. The Great gene is highly expressed in the gubernaculum, the ligament that controls testicular movement during development, and therefore may be responsible for mediating hormonal signals that affect testicular descent. Here we show that genetic targeting of the Great gene in mice causes infertile bilateral intraabdominal cryptorchidism. The mutant gubernaculae fail to differentiate, indicating that the Great gene controls their development. Mutation screening of the human GREAT gene was performed using DHPLC analysis of the genomic DNA from 60 cryptorchid patients. Nucleotide variations in GREAT cDNA were found in both the patient and the control populations. A unique missense mutation (T222P) in the ectodomain of the GREAT receptor was identified in one of the patients. This mutant receptor fails to respond to ligand stimulation, implicating the GREAT gene in the etiology in some cases of cryptorchidism in humans.

Ivan P. Gorlov; Aparna Kamat; Natalia V. Bogatcheva; Eric Jones; Dolores J. Lamb; Anne Truong; Colin E. Bishop; Ken Mcelreavey; Er I. Agoulnik

2002-01-01T23:59:59.000Z

342

Hormonal activation of genes through nongenomic pathways by estrogen and structurally diverse estrogenic compounds  

E-Print Network (OSTI)

Lactate dehydrogenase A (LDHA) is hormonally regulated in rodents, and increased expression of LDHA is observed during mammary gland tumorigenesis. The mechanisms of hormonal regulation of LDHA were investigated in breast cancer cells using a series of deletion and mutant reporter constructs derived from the rat LDHA gene promoter. Results of transient transfection studies showed that the -92 to -37 region of the LDHA promoter was important for basal and estrogen-induced transactivation, and mutation of the consensus CRE motif (-48/-41) within this region resulted in significant loss of basal activity and hormone-responsiveness. Gel mobility shift assays using nuclear extracts from MCF-7 cells indicated that CREB family proteins interacted with the CRE. Studies with kinase inhibitors showed that estrogen-induced activation of this CRE was dependent on protein kinase C, and these data show that LDHA is induced through a nongenomic (extranuclear) pathway of estrogen action. Estrogen activates several nongenomic pathways in MCF-7 cells, and this study investigated the effects of structurally diverse estrogenic compounds on activation of mitogen activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K), protein kinase C (PKC), protein kinase A (PKA), and calcium/calmodulin-dependent protein kinase IV (CaMKIV). Activation of kinases was determined by specific substrate phosphorylation and transactivation assays that were diagnostic for individual kinases. The compounds investigated in this study include E2, diethylstilbestrol (DES), the phytoestrogen resveratrol, and the following synthetic xenoestrogens: bisphenol-A (BPA), nonylphenol, octylphenol, endosulfan, kepone, 2,2-bis(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE), and 2',3',4',5'-tetrachloro-4-biphenylol (HO-PCB-Cl4). With theexception of resveratrol, all the compounds activated PI3K and MAPK whereas activation of PKC by the xenoestrogens was structure-dependent and resveratrol, kepone and HO-PCB-Cl4 were inactive. Only minimal estrogen/xenoestrogen-dependent activation of PKA was observed. CaMKIV was activated only by E2 and DES, and HO-PCB-Cl4 was a potent inhibitor of CaMKIV-dependent activity. These results demonstrate that activation of nongenomic pathways by estrogenic compounds in MCF-7 cells is structure-dependent.

Li, Xiangrong

343

GenePRIMP: A GENE PRediction IMprovement Pipeline for Prokaryotic genomes  

E-Print Network (OSTI)

PRediction IMprovement Pipeline for Amrita Pati 1 , NataliaGene Prediction IMprovement Pipeline, http://geneprimp.jgi-based post-processing pipeline that identifies erroneously

Pati, Amrita

2012-01-01T23:59:59.000Z

344

Post transcriptional regulation of chloroplast gene expression by nuclear encoded gene products  

DOE Green Energy (OSTI)

Many individual chloroplast genes require the products of a collection of nuclear genes for their successful expression. These nuclear gene products apparently work with great specificity, each committed to the expression of a single chloroplast gene. We have chosen as a model nuclear mutants of Chlamydomonas affected in different stages in the expression of the chloroplast encoded Photosystem II polypeptide, D2. We have made the progress in understanding how nuclear gene products affect the translation of the D2 encoding MRNA. Two nuclear genes are required for this process which have been mapped genetically. In contrast to other examples of nuclear control of translation in the chloroplast, these nuclear gene products appear to be required either for specific stages in translation elongation or for the post-translational stabilization of the nascent D2 protein. Pseudoreversion analysis has led us to a locus which may be directly involved in D2 expression. We have made considerable progress in pursuing the molecular basis of psbd MRNA stabilization. psbD 5' UTR specific transcripts have been synthesized in vitro and used in gel mobility shift assays. UV-crosslinking studies are underway to identify the transacting factors which bind to these sequences. The continued examination of these mutants will help us to understand how nuclear gene products work in this specific case of chloroplast gene expression, and will elucidate how two distinct genomes can interact generally.

Kuchka, M.R.

1992-01-01T23:59:59.000Z

345

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advanced nuclear energy systems.” Zinkle was also named a TMS Fel- low in 2011. Steven Zinkle. Nominations Open for AIME James Douglas Medal.

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Member. News. In Memory of Ralph L. Harris, 1954—2007. In July, Ralph L. Harris, a professor in the Department of Mining and Mate- rials Engineering at ...

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scale. His continual research has led to major advances in the understanding of ... thermoelectricity, and the new physics at the nanometer scale have led to nu-.

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founded the company, Charles Martin. Hall. “I think if Charles Martin Hall were here today he would be proud that his patent pretty much still describes the.

349

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fairs, Illinois Institute of Technol- ogy. ? Gary L. Doll: chief technologist,. Tribology , The Timken Co. ? Donald U. Gubser: superintend- ent, Materials Science and ...

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partments on Methane HydratesiAn. Alternate Energy Source. The Hollomon Award consists of a glass sculpture, an inscribed certi?cate, and acash honorarium.

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thetic Coal-Petcoke Slags (Al2O3-CaO-. FeO-SiO2-V2O3) under Simulated Gasifi - cation Conditions,” published in Energy and Fuels. 2012 Class of Fellows.

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Ibrahim Karaman Appointed to Chevron Professorship. Ibrahim Karaman. Garry Warren Appointed AIME President-Elect. The TMS Board of Directors has.

353

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solutions to the world's energy chal- lenge. Herderick has been working to improve the way energy is generated, .... of Texas at Austin, travels yearly to Ja-.

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Ready's work on cost-effective, ener- gy-efficient photovoltaic cells made of lightweight carbon nanotubes. Ready's experiment will make use of the NanoRacks ...

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University of Pennsylvania's School of. Engineering and Applied Science and the Wharton School. Her development of CermeTi has opened the door to many ...

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A host of social events will be organized, including a conference banquet, networking reception, and an afternoon excursion. Also, industrial tours will be offered, ...

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Energy Committee (LMD/EPD). Topics. This symposium will investigate the recovery and ... Regenerative systems. • Recuperators. • Self-recuperative burners.

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test reactor to improve nuclear reactor performance ... of engineering physics at the Univer- sity of Wisconsin at ... the new user facility on the Nuclear. Materials ...

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ics and a master's degree in Nuclear. Physics at the University of British. Columbia, he earned a doctorate in. Nuclear Physics at McGill University. He served as ...

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Applied Physics A: Materials. Science and Processing. ? Applied Physics B: Lasers and. Optics ... and the Indian Nuclear Society, and was designated a fellow ...

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the availability of safe drinking water, adequate sanitation and hygiene, and related education through sustainable development projects, technology, and.

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of Petroleum Engineers (SPE), and Roy. H. Koerner, [995 SPE president and. 1998 president of the American Institute of Mining, Metallurgical and Petroleum.

363

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The archives of AIME Transactions, the long-running publication of the. American Institute of Mining, Met- allurgical, and Petroleum Engineers. (AIME), are now ...

364

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and Petroleum Engineers. The archive has 247 volumes of papers published between 1871–1970. TMS MEMBER RECEIVES. FELLOW OF ABET AWARD.

365

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Mining, Metallurgical, and Petroleum. Engineers (AIME); the International. Marine Minerals Society (IMMS); the. National Institute for Occupational. Safety and ...

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Mar 11, 2012 ... in the transmission electron microscope to perform quantitative studies of dislo- cation defects in metals and intermetal- lic alloys,” said Dennis ...

367

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chi, senior research scientist, Hydro-. Quebec Research Institute; and Nathan. Stubina, Research and Development manager, Barrick Gold Corporation.

368

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Motivation; Passion and ICE (Integrity, Courage, and Empathy); Risk Management;. Social Styles/Personality Assessment; Traits of Leadership. The ELA on ...

369

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in Montreal, Canada, passed away sud— denly at the age of 52. .... of atomic interactions rapidly becomes so complex that using it to predict the properties and ...

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search capability that enables users to browse by volume, or search by author, title, keyword(s), proceedings title or year, or title keyword. All articles also have.

371

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search award for his work on the quenching of spin ?uctuations and in- ducing magnetic moments in meta- magnetic materials. The Acta Materialia Gold Medal.

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eder, president of Energy Valley, a con- sulting firm ... director for Engineering Trends, an e- commerce ... fixed prices for LPs, making them cost- prohibitive for

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Lithium and Rare Earth Metals. • Other Metals. Processes: • Hydrometallurgy. - Leaching. - Solution Purification, including SX and IX. - Product Recovery.

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toes, cucumbers, green chile peppers, and green beans. She also has peach, pear, sour cherry, apple, apricot, and plum trees. However, not all of the fruit.

375

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of uranium enrichment. In the story,. Marvin Khorbin, a brilliant young sci- entist who pioneered the development of the sublimation process, is murdered.

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creation of new insight; a world ambas- sador; and an innovator and ... in the world, Conchita Martinez. (Below): Hof- mann in ... The only ac- complishment I look ...

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Committee web page, which can be ac- cessed through the “Committee Home ... considered one of the world's leading authorities in atom-probe tomogra-.

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the laboratory's reputation as a world- wide leader in ... materials from the University of Ox- ford in England. p g r. S i. P w a. S. Boa the. Ac ha be. 19 on. B to a p.

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in research to solve challenging prob- lems confronting the world today. That ... birthday in April 2013, is still very ac- tive in research as the Technological.

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White House ceremony and receive a. $10,000 grant to continue to advance their mentoring work. William Nix Recognized for Outstanding Materials Research.

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one-story house that he designed and had built from scratch to the specific needs of his family. “I picked a plan out of a book and modified it quite a bit,” said DeY ...

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Menu items and prices may be subject to change without notice. ... All prices are subject to a (++) charge, which represents a 19% service charge and California ...

383

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Anderson Leads Research on Magnets for Electric Motors. TMS Member Iver Anderson is lead- ing a research team at the U.S. Depart- ment of Energy's Ames

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during a compelling discussion of how better batteries can lead to world peace ... battery prototype during Sadoway's ... have batteries that can bring renewable.

385

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Fossil fuel energy uses always release water vapor in the flue gases and is a thermal emission which needs to be controlled while addressing Climate change,

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Organizers: N. Chawla (Arizona State University, United States), R.D. Toledo Filho (Universidade Federal do Rio de Janeiro, Brazil), K.K. Chawla (University of

387

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cess engineer with Union Oil Company and Fractionation Research Incorporat ... area of rare earth production and sepa- ration. He then went on to earn a Ph.D.

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jointly at Ford Motor Company and the University of Michigan, and a se- nior development engineer at Hoegan- aes Corporation. His research involves.

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The congress will be held at the Intercontinental Rio Hotel. ... important technological applications in transportation (e.g., crash worthiness of vehicles), energy.

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nel surfing he landed on the Le Tour de. France. He had heard of the annual cy- cling event and knew who Lance Arm- strong was, but never saw the nearly.

391

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Also, industrial tours will be offered, as well as a number of local sight-seeing tours (Ilha Grande, Parati). Details will be added to the. 2010 TMS-ABM conference ...

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40 years of age whose paper has been selected as the best of those ... 2012 Albert Easton White Distinguished. Teacher Award .... “We need to ad- dress the

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director of the Quad Cities Manufac- turing Laboratory, a nonprofit research and development company in the Rock. Island Arsenal—Joint Manufactur- ing and ...

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Inc. He later became chair of the Acta Board of Governors and led the organization until his death. Scott earned his B.S. in Metallurgical Engineering from ...

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His continual research has led to major advances in the understanding of the behavior of vacancies and self-in- terstitial atoms in metals. Early in his career, ...

396

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in 2000, he led the development of solid oxide fuel cell technology from a laboratory curiosity to fully integrated. 200 kilowatt power-generation sys- tems while at ...

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TMS Member Profiles. Laura Rea has played a variety of roles in her life: the ... “ An engineering course load and later marriage and a family didn't leave a lot of ...

398

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Narayan, John C. C. Fan Family Dis- tinguished Chair. Professor, Depart- ment of Materials,. North. Carolina. State University. (NCSU), and win- ner of the 2011.

399

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C. Fan Family Distinguished Chair in Materi- als Science, North Carolina State University. The occasion turned into a reunion of TMS members who have earned ...

400

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diversity. Jones Selected as First Materials Societies Fellow. A polymer expert with an inter- est in science policy development has been selected to receive the  ...

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Dayton, Ohio. His research interests lie in high-temperature deformation, ma- terials behavior modeling, and materi- als engineering. Srinivasan received.

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Omer Dogan, DOE National Energy Technology Laboratory, USA / omer.dogan@ netl.doe.gov. Jim Ciulik, University of Texas, Austin, USA / jciulik@mail.utexas.

403

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research and head of the Materials Sci- ence and Component Technology Di- rectorate at the Naval Research Labo- .... medical equipment cabinets were too.

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to put more heat on a certain part of the piece,” he said. “They try to interact ... blow pipe and then try to propagate the right pressure of air as it moves to the.

405

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Carl Boehlert, Michigan State University (1997). Thomas ... Robert Hyers, University of Massachusetts (1994) ... Fuqian Yang, University of Kentucky (1995)

406

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all served so well. David R. Gaskell. An exceptionally involved member in the TMS Extraction & Processing. Division (EPD),. David R. Gaskell passed away on.

407

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billion Spallation Neutron Source, and his tenure also included the completion of the Center for Nanophase Materials. Science and responsibility for the U.S..

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/General/2012AwardNominationForm. pdf. To review the guidelines, go to http:// www.aimehq.org/sites/default. /files/aime_guidelines_douglas. _award.pdf.

409

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developing fields of technology, mak- ing major advancements in traditional fields of engineering, or developing/ implementing innovative approaches.

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Dawn Janney: Idaho Falls, Idaho; materials engineer, Idaho National. Laboratory; Professional Mem- ber. • Rejin Koodakal: Thun, Switzer- land; post doctorate ...

411

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Allen Named Director of Advanced Test Reactor User Facility at INL. TMS Member Todd Allen has been selected by Idaho National Laboratory. (INL) to serve as.

412

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AES/MOM Feedback AES/MOM Feedback First Name Last Name E-Mail Comment Response Initials Date Bob Wilson wilson@aps.anl.gov I was called in on Sunday July 21. I had to go up to the control room to get the key so I could get into our tech room. My keys and other items I need to perform my work are at my desk so I must have access to it. The first guy in the control room didn't even know what I was talking about when I asked him for the key. Luckily a second person walked in as I was about to leave and he knew about the key. This senerio took a little over 30 minutes of time after I arrived at the lab. The problem I was called in for was ours so I'm sure that our group is charged with this downtime for the user. I don't understand why I don't have access to my own work area. I only have it if I ask someone to let me in. The talk around the APS is how we are all in a budget crisis. I'm told that we are eventually getting a card reader on one of the doors but the cost of that is in the neighborhood of $6,000.00. Why do we not all just get keys? It would save thousands of dollars and time in the event we are called in during off hours. I agree with you Bob. The installation delay for a badge card reader is due to the required parts not being available. This has now been resolved (I think) and Scheck can now go forward with the installation. Thanks for your patience with this annoying situation. Geo 2013-07-26 08:44:43

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sor of Materials and Metallurgi- cal Engineering at South Dakota. School of Mines and Technology. He will guide the professional activities of TMS concerned ...

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turing at the South Dakota School of. Mines & Technology and executive director of the Quad Cities Manufac- turing Laboratory, a nonprofit research.

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tional security in an environmentally sustainable manner. During his ... the nuclear-warhead-armed Minuteman ... reactor design, and demonstration of superior ...

416

Gene for ataxia-telangiectasia complementation group D (ATDC)  

DOE Patents (OSTI)

Disclosed herein is a new gene, an AT gene for complementation group D, the ATDC gene and fragments thereof. Nucleic acid probes for said gene are provided as well as proteins encoded by said gene, cDNA therefrom, preferably a 3 kilobase (kb) cDNA, and recombinant nucleic acid molecules for expression of said proteins. Further disclosed are methods to detect mutations in said gene, preferably methods employing the polymerase chain reaction (PCR). Also disclosed are methods to detect AT genes from other AT complementation groups.

Murnane, John P. (San Francisco, CA); Painter, Robert B. (Burlingame, CA); Kapp, Leon N. (San Rafael, CA); Yu, Loh-Chung (Redwood City, CA)

1995-03-07T23:59:59.000Z

417

Gene for ataxia-telangiectasia complementation group D (ATDC)  

DOE Patents (OSTI)

Disclosed herein is a new gene, an AT gene for complementation group D, the ATDC gene and fragments thereof. Nucleic acid probes for the gene are provided as well as proteins encoded by the gene, cDNA therefrom, preferably a 3 kilobase (kb) cDNA, and recombinant nucleic acid molecules for expression of the proteins. Further disclosed are methods to detect mutations in the gene, preferably methods employing the polymerase chain reaction (PCR). Also disclosed are methods to detect AT genes from other AT complementation groups. 30 figs.

Murnane, J.P.; Painter, R.B.; Kapp, L.N.; Yu, L.C.

1995-03-07T23:59:59.000Z

418

A CSP View on UMLRT structure diagrams Clemens Fischer, ErnstRudiger Olderog and Heike Wehrheim  

E-Print Network (OSTI)

A CSP View on UML­RT structure diagrams Clemens Fischer, Ernst­R¨udiger Olderog and Heike Wehrheim UML­RT structure diagrams together with the formal method CSP­OZ combining CSP and Object­Z. While CSP­OZ is used for specifying the system components themselves (by CSP­OZ classes), UML­RT diagrams provide

Olderog, Ernst-Rüdiger

419

Conservation Focus: Priorities for Policy-Relevant Conservation Research: a View from SCB Regional Sections  

E-Print Network (OSTI)

Conservation Focus: Priorities for Policy-Relevant Conservation Research: a View from SCB Regional Sections Gaining Traction: Retreading the Wheels of Marine Conservation PHAEDRA DOUKAKIS, E. C. M. PARSONS Conservation Science, School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794

420

Some modifications of bucket-based algorithms for query rewriting using views  

Science Conference Proceedings (OSTI)

Query rewriting using views is an important topic in data integration. A number of rewriting algorithms, such as the SVB algorithm, the MiniCon algorithm and the inverse rules algorithm, have been developed. All the algorithms can generate a maximally-contained ...

Qingyuan Bai; Jun Hong; Michael F. McTear

2004-10-01T23:59:59.000Z

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421

PowerView monitoring system for instant power consumption in tritium separation technological installations  

Science Conference Proceedings (OSTI)

The paper shows the development of some integrated systems for monitoring and control of instant power consumption in normal and abnormal operation mode of tritium separation technological installations, with the view of reducing energy losses, supports ... Keywords: monitoring systems, power consumption

Carmen Maria Moraru; Ciprian Ciprian Bucur; Iulia Stefan; Ovidiu Balteanu

2011-07-01T23:59:59.000Z

422

SOFAR Floats Reveal Midlatitude Intermediate North Atlantic General Circulation. Part II: An Eulerian Statistical View  

Science Conference Proceedings (OSTI)

Part I of this paper has given a descriptive view of the trajectories of 26 SOFAR floats drifting near 700-m depth in the central North Atlantic during the mid-1980s, as part of the TOPOGULF experiment. Here an Eulerian analysis of the 53.4 ...

Michel Ollitrault; Alain Colin de Verdière

2002-07-01T23:59:59.000Z

423

Deconvolution of Wide-Field-of-View Measurements of Reflected Solar Radiation  

Science Conference Proceedings (OSTI)

Wide-field-of-view (WFOV) radiometers have been flown as part of the Earth Radiation Budget instrument on the Nimbus 6 and 7 spacecraft and as part of the Earth Radiation Budget Experiment (ERBE) instruments aboard the ERBE spacecraft and also ...

G. Louis Smith; David Rutan

1990-02-01T23:59:59.000Z

424

NATURE PHYSICS | VOL 9 | DECEMBER 2013 | www.nature.com/naturephysics 755 news & views  

E-Print Network (OSTI)

power plants. William Morris is at the Culham Centre for Fusion Energy, Abingdon, Oxfordshire, OX14 3DBNATURE PHYSICS | VOL 9 | DECEMBER 2013 | www.nature.com/naturephysics 755 news & views waves on another key aspect of tokamaks -- the power exhaust. Most tokamaks have so-called divertors where plasma

Loss, Daniel

425

A more complex view of schedule uncertainty based on contingency analysis  

E-Print Network (OSTI)

. · Search by expressions and keywords · A good electronic dictionary The application should run under MS significant additional commercial and technical risks for the project. From the NLE point of view. incremental search of the type offered in standard 'help' systems is made available to the user The hierarchy

Massachusetts at Amherst, University of

426

Sky View Factors from High-Resolution Scanned Fish-eye Lens Photographic Negatives  

Science Conference Proceedings (OSTI)

A computerized method for calculating the sky view factor from fish-eye lens photographic negatives is presented. The images are scanned and stored on CD ROM, each CD holding 100 images. The images can be retrieved at very high resolutions of up ...

Kristina Blennow

1995-12-01T23:59:59.000Z

427

Parallel view-dependent tessellation of Catmull-Clark subdivision surfaces  

Science Conference Proceedings (OSTI)

We present a strategy for performing view-adaptive, crack-free tessellation of Catmull-Clark subdivision surfaces entirely on programmable graphics hardware. Our scheme extends the concept of breadth-first subdivision, which up to this point has only ... Keywords: Catmull-Clark, GPGPU, adaptive surface subdivision, subdivision surfaces

Anjul Patney; Mohamed S. Ebeida; John D. Owens

2009-08-01T23:59:59.000Z

428

Study of Elevator Safety Performance Test System Based on LabVIEW  

Science Conference Proceedings (OSTI)

The upgrading of China's coal mine detection equipment and technical means are very backward, mainly distributed instruments and devices, low efficiency, poor accuracy, detection and testing of the limitations of the data, testing methods, data-processing ... Keywords: Elevator, LabVIEW, test system

Bingsheng Wu; Chaozhi Cai

2009-11-01T23:59:59.000Z

429

DTC Open Day, 25th February 2009 Wind energy consultancy: a personal view  

E-Print Network (OSTI)

DTC Open Day, 25th February 2009 Wind energy consultancy: a personal view Peter Clive Technical;Overview of Capability Wind · Due diligence · Lenders engineer · Energy yields · Technology audits and due diligence #12;Wind energy activities · Pre-feasibility · Wind monitoring · Wind regime analysis

Mottram, Nigel

430

Integrated Google Maps and Smooth Street View Videos for Route Planning National Taiwan University  

E-Print Network (OSTI)

Integrated Google Maps and Smooth Street View Videos for Route Planning Chi Peng National Taiwan that takes start and end points as the input, and will automatically connect to the Google Maps with Street a smooth scenic video from the starting point to the destination, which combines the Google Maps to provide

Ouhyoung, Ming

431

Study of Safety Auditors' Views on the Use of BIM for Safety in Hong Kong  

Science Conference Proceedings (OSTI)

Traditionally site safety is a concern for the Architectural, Engineering & Construction AEC industry. In view of government of Hong Kong initiating a number of large scale AEC projects and a relative high number of serious accidents continue to ... Keywords: Architectural Engineering & Construction AEC, Building Information Model BIM, Hong Kong, Safety Management System SMS, Sustainable Development SD

Allen Wan, Andrew Platten, Tim Briggs

2013-01-01T23:59:59.000Z

432

On Symmetry and Multiple-View Geometry: Structure, Pose, and Calibration from a Single Image  

Science Conference Proceedings (OSTI)

In this paper, we provide a principled explanation of how knowledge in global 3-D structural invariants, typically captured by a group action on a symmetric structure, can dramatically facilitate the task of reconstructing a 3-D scene from one or more ... Keywords: multiple-view geometry, reflective symmetry, rotational symmetry, structure from symmetry, symmetry group, translational symmetry

Wei Hong; Allen Yang Yang; Kun Huang; Yi Ma

2004-12-01T23:59:59.000Z

433

Restructuring Batch View Maintenance Efficiently Bin Liu, Elke A. Rundensteiner and David Finkel  

E-Print Network (OSTI)

with existing batch algorithms. Categories and Subject Descriptors: H.2.4 [Database Management]: Systems maintenance strategies within the TxnWrap system [3]. The experiments are conducted on four Pentium III 500MHz Systems (TODS), 2004, to appear. [4] A. Gupta and I. Mumick. Maintenance of Materialized Views: Problems

434

The Universe Viewed in Gamma-Rays 1 Pachmarhi Array of Cerenkov Telescopes  

E-Print Network (OSTI)

The Universe Viewed in Gamma-Rays 1 Pachmarhi Array of Cerenkov Telescopes P.Majumdar, B of Cerenkov Telescopes (PACT) has been designed to search for celestial TeV -rays using the wavefront sampling Atmospheric Cerenkov technique is the only method which has been suc- cessfully used to probe the sky

Enomoto, Ryoji

435

A single-view based framework for robust estimation of height and position of moving people  

Science Conference Proceedings (OSTI)

In recent years, there has been increased interest in characterizing and extracting 3D information from 2D images for human tracking and identification. In this paper, we propose a single view-based framework for robust estimation of height and position. ... Keywords: height estimation, human tracking, position estimation, video surveillance

Seok-Han Lee; Jong-Soo Choi

2007-12-01T23:59:59.000Z

436

Nuclear Waste Disposal: An Independent View of the Big Picture and a Proposal for CARD  

E-Print Network (OSTI)

1 Nuclear Waste Disposal: An Independent View of the Big Picture and a Proposal for CARD Presented to isolate nuclear waste successfully from the biosphere for the long term can be developed if our society to this impasse? In the 1940's at the beginning of the nuclear age, nuclear waste was seen as a "problem" only

California at Santa Cruz, University of

437

Semantics of model views for information exchanges using the industry foundation class schema  

Science Conference Proceedings (OSTI)

The industry foundation classes (IFC) data schema is generic, designed to support the full range of model exchanges needed in the construction industry. For any particular working exchange for some sub-domain of building construction, a set of model ... Keywords: Industry foundation classes (IFC), Interoperability, Model view definitions (MVD), National BIM Standard (NBIMS), Process modeling, Product modeling

M. Venugopal; C. M. Eastman; R. Sacks; J. Teizer

2012-04-01T23:59:59.000Z

438

Efficient view-dependent out-of-core visualization Michael Guthe, Pavel Borodin and Reinhard Klein  

E-Print Network (OSTI)

node a level of detail is generated for efficient view-dependent rendering. To ensure correct matching algorithm with appropriately shaded fat borders. Furthermore, several minor yet important improvements-dependent rendering technique which allows for the natural incorporation of state-of-the-art culling, simplification

Behnke, Sven

439

22nd Conference on Hydrology A Satellite View of Global Water and Energy Cycling  

E-Print Network (OSTI)

Water and Energy Cycling (2008 - 88Annual_22hydro) 2/6/2008http://ams.confex.com/ams/88Annual22nd Conference on Hydrology 8.1 A Satellite View of Global Water and Energy Cycling Paul R. Houser in modeling capability, satellite observations have great potential to make huge advances in water and energy

Houser, Paul R.

440

Soft computing methods to predict gene regulatory networks: An integrative approach on time-series gene expression data  

Science Conference Proceedings (OSTI)

To unravel the controlling mechanisms of gene regulation, in this paper we present the application of sophisticated soft computing methods applied on an important problem from Bioinformatics-inferring gene regulatory networks (GRN) from time series gene ... Keywords: Automatic model building, Gene regulatory network, LARS, Reverse-engineering, Schizosaccharomyces pombe, Time series microarray data, Yeast

Zeke S. H. Chan; Ilkka Havukkala; Vishal Jain; Yingjie Hu; Nikola Kasabov

2008-06-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

A Rule-Based Framework for Gene Regulation Pathways Discovery  

SciTech Connect

We present novel approach to discover the rules that govern gene regulation mechanisms. The method is based on supervised machine learning and is designed to reveal relationships between transcription factors and gene promoters. As the representation of the gene regulatory circuit we have chosen a special form of IF-THEN rules associating certain features (a generalized idea of a Transcription Factor Binding Site) in gene promoters with specific gene expression profiles.

Wilczynski, B; Hvidsten, T; Kryshtafovych, A; Stubbs, L; Komorowski, J; Fidelis, K

2003-07-21T23:59:59.000Z

442

Pathogenicity island mobility and gene content.  

SciTech Connect

Key goals towards national biosecurity include methods for analyzing pathogens, predicting their emergence, and developing countermeasures. These goals are served by studying bacterial genes that promote pathogenicity and the pathogenicity islands that mobilize them. Cyberinfrastructure promoting an island database advances this field and enables deeper bioinformatic analysis that may identify novel pathogenicity genes. New automated methods and rich visualizations were developed for identifying pathogenicity islands, based on the principle that islands occur sporadically among closely related strains. The chromosomally-ordered pan-genome organizes all genes from a clade of strains; gaps in this visualization indicate islands, and decorations of the gene matrix facilitate exploration of island gene functions. A %E2%80%9Clearned phyloblocks%E2%80%9D method was developed for automated island identification, that trains on the phylogenetic patterns of islands identified by other methods. Learned phyloblocks better defined termini of previously identified islands in multidrug-resistant Klebsiella pneumoniae ATCC BAA-2146, and found its only antibiotic resistance island.

Williams, Kelly Porter

2013-10-01T23:59:59.000Z

443

The mouse angiogenin gene family: Structures of an angiogenin-related protein gene and two pseudogenes  

SciTech Connect

Angiogenin, a homologue of pancreatic ribonuclease, is a potent inducer of blood vessel formation. As an initial step toward investigating the in vivo functional role of this protein via gene disruption, we undertook the isolation of the angiogenin gene (Ang) from the 129 strain mouse, which will be used for generating targeting constructs. Unexpectedly, screening of a genomic library with an Ang gene probe obtained previously from the BALB/c strain yielded two new genes closely similar to Ang rather than Ang itself. One of these encodes a protein with 78% sequence identity to angiogenin and is designated {open_quotes}Angrp{close_quotes} for {open_quotes}angiogenin-related protein.{close_quotes} The ribonucleolytic active site of angiogenin, which is critical for angiogenic activity, is completely conserved in Angrp, whereas a second essential site, thought to bind cellular receptors, is considerably different. Thus, the Angrp product may have a function distinct from that of angiogenin. The second gene obtained by library screening is a pseudogene, designated {open_quotes}Ang-ps1,{close_quotes} that contains a frame shift mutation in the early part of the coding region. Although the Ang gene was not isolated from this library, it was possible to amplify this gene from 129 mouse genomic DNA by the polymerase chain reaction (PCR). Sequence analysis showed that the 129 strain Ang gene is identical to the BALB/c gene throughout the coding region. PCR cloning also yielded a second Ang-like pseudogene, designated {open_quotes}Ang-ps2.{close_quotes} Southern blotting of genomic DNA confirmed the presence of Ang, Angrp, and at least one of the pseudogenes in an individual mouse and suggested that the mouse Ang gene family may contain more than the four members identified here. 31 refs., 4 figs., 1 tab.

Brown, W.E.; Nobile, V.; Shapiro, R. [Harvard Medical School, Boston, MA (United States)] [and others

1995-09-01T23:59:59.000Z

444

(for Gene Recognition Analysis Internet Link),  

NLE Websites -- All DOE Office Websites (Extended Search)

5 6/1/2011 5 6/1/2011 6.6 Speeding Up the Process of Gene Discovery The human genome contains information that could be used to prevent birth defects and treat or cure devastating diseases, but it is written in a language that scientists are only beginning to understand. To help decipher the code, Ed Uberbacher and colleagues at Oak Ridge National Laboratory combined cutting- edge computer technology with their knowledge of human biology to develop GRAIL (for Gene Recognition Analysis Internet Link), a "thinking" computer program that imitates the human learning process as it searches for genetic meaning. GRAIL and successor software programs can rapidly identify key instructions in genes from within vast stretches of DNA that appear to be meaningless-a critical contribution to the

445

Regulation of methane genes and genome expression  

DOE Green Energy (OSTI)

At the start of this project, it was known that methanogens were Archaeabacteria (now Archaea) and were therefore predicted to have gene expression and regulatory systems different from Bacteria, but few of the molecular biology details were established. The goals were then to establish the structures and organizations of genes in methanogens, and to develop the genetic technologies needed to investigate and dissect methanogen gene expression and regulation in vivo. By cloning and sequencing, we established the gene and operon structures of all of the “methane” genes that encode the enzymes that catalyze methane biosynthesis from carbon dioxide and hydrogen. This work identified unique sequences in the methane gene that we designated mcrA, that encodes the largest subunit of methyl-coenzyme M reductase, that could be used to identify methanogen DNA and establish methanogen phylogenetic relationships. McrA sequences are now the accepted standard and used extensively as hybridization probes to identify and quantify methanogens in environmental research. With the methane genes in hand, we used northern blot and then later whole-genome microarray hybridization analyses to establish how growth phase and substrate availability regulated methane gene expression in Methanobacterium thermautotrophicus ?H (now Methanothermobacter thermautotrophicus). Isoenzymes or pairs of functionally equivalent enzymes catalyze several steps in the hydrogen-dependent reduction of carbon dioxide to methane. We established that hydrogen availability determine which of these pairs of methane genes is expressed and therefore which of the alternative enzymes is employed to catalyze methane biosynthesis under different environmental conditions. As were unable to establish a reliable genetic system for M. thermautotrophicus, we developed in vitro transcription as an alternative system to investigate methanogen gene expression and regulation. This led to the discovery that an archaeal protein, designated TFE, that had sequences in common with the eukaryotic general transcription factor TFIIE, stimulated archaeal transcription initiation and that the archaeal TATA-box binding protein (TBP) remained attached to the promoter region whereas the transcription factor TFB dissociated from the template DNA following initiation. DNA sequences that directed the localized assembly of archaeal histones into archaeal nucleosomes were identified, and we established that transcription by an archaeal RNA polymerase was slowed but not blocked by archaeal nucleosomes. We developed a new protocol to purify archaeal RNA polymerases and with this enzyme and additional improvements to the in vitro transcription system, we established the template requirements for archaeal transcription termination, investigated the activities of proteins predicted to be methane gene regulators, and established how TrpY, a novel archaeal regulator of expression of the tryptophan biosynthetic operon functions in M. thermautotrophicus. This also resulted in the discovery that almost all M. thermautotrophicus mutants isolated as spontaneously resistant to 5-methyl tryptophan (5MTR) had mutations in trpY and were therefore 5MTR through de-repressed trp operon expression. This established a very simple, practical procedure to determine and quantify the DNA sequence changes that result from exposure of this Archaeon to any experimental mutagenesis protocol. Following the discovery that the Thermococcus kodakaraensis was amenable to genetic manipulation, we established this technology at OSU and subsequently added plasmid expression, a reporter system and additional genetic selections to the T. kodakaraensis genetic toolbox. We established that transcription and translation are coupled in this Archaeon, and by combining in vitro transcription and in vivo genetics, we documented that both TFB1 and TFB2 support transcription initiation in T. kodakaraensis. We quantified the roles of ribosome binding sequences and alternative initiation codons in translation initiation, established that polarity e

John N. Reeve

2009-09-09T23:59:59.000Z

446

Gene Expression in the Stallion Testes  

E-Print Network (OSTI)

Understanding the genes that regulate spermatogenesis and steroidogenesis in the testis is critical for enhancement of stallion fertility. Stallion testicular samples were used to identify candidate genes by cDNA microarrays that simultaneously assessed expression levels of 9132 genes. First, gene expression was compared between light (spermatogenically active) and dark (spermatogenically inactive) testis tissue of 1.5-year-old horses (n = 3). Ninety-three genes were differentially expressed (35 light specific, 58 dark specific) in matched paired samples. Second, gene expression was compared between testicular tissue of two mature stallions, one with normal quality semen (fertile) and one with poor quality semen (subfertile). A total of 233 genes were differentially expressed (122 in fertile tissue, 111 in subfertile tissue). Of these, phosphodiesterase 3B (PDE3B), steroidogenic acute regulatory (StAR) protein, and outer dense fiber of sperm tails 2 (ODF2) mRNAs, were localized and quantified by in situ hybridization (ISH) in mature stallions and/or in unilateral cryptorchids. ISH revealed differences (P < 0.05) among mature stallions (n = 10) for PDE3B (localized to seminiferous tubules) and StAR protein (localized to interstitial spaces) mRNAs. A positive correlation coefficient (r = .556, p = .025) was found between StAR protein mRNA and plasma concentration of testosterone. Additionally, both gene products were evaluated in 1-year-old (n = 3) and 3-year-old (n = 3) unilateral cryptorchid stallions. Expression of both PDE3B and StAR protein gene was significantly higher in mature, descended testes compared to mature, retained testes and the descended and retained testes of immature, cryptorchid stallions. StAR protein gene demonstrated significantly higher expression in immature retained testes compared to immature descended testes. A precision-cut tissue slice (PCTS) in vitro culture system was evaluated as a potential tool to study equine testes function. Testes from immature stallions (n = 3) were cut into slices (mean slice weight = 13.85 +/- 0.20 mg; mean slice thickness = 515.00 +/- 2.33 ?m) and exposed to medium containing ovine luteinizing hormone (oLH) at concentrations of 0, 5, 50 and 500 ng/ml for 6 h at 32 degrees C. Medium content of testosterone and estradiol was increased 500% and 120%, respectively, by addition of oLH versus that observed for the testis tissue slices treated with 0 ng oLH (control). An oLH concentration-dependent increase in StAR protein mRNA in tissue slices was detected by in situ hybridization; whereas, differences for PDE3B and ODF2 mRNAs were not observed. Collectively, these results demonstrate that the stallion is an excellent model for studying male fertility due to the initiation of spermatogenesis, frequency of cryptorchidism, and routine castration providing useful tissue to use for studying gene expression.

Laughlin, Andy M.

2010-05-01T23:59:59.000Z

447

Undergraduate Research at Jefferson Lab - LabVIEW Software Based Program to  

NLE Websites -- All DOE Office Websites (Extended Search)

Data Acquisition Components Data Acquisition Components Previous Project (Data Acquisition Components) Undergraduate Research Main Index Next Project (Spin-Polarization of Helium-3 Target Cell) Spin-Polarization of Helium-3 Target Cell LabVIEW Software Based Program to Minimize Data File Size of the Slow Controls System (SCS) of the Silicon Vertex Tracker (SVT) Student: Zulqarnain M Jamal School: Georgia Institute of Technology Mentored By: Amrit Yegneswaran SVT is a detector, made of silicon modules. It tracks charged particles and helps to determine interaction vertices. SCS controls and monitors currents and voltages, and monitors temperatures and humidity. SCS produces large data files. Smart Logger, a LabVIEW program, has been developed in to minimize data file size. Smart Logger discards data-sets if any the

448

Grand View-on-Hudson, New York: Energy Resources | Open Energy Information  

Open Energy Info (EERE)

View-on-Hudson, New York: Energy Resources View-on-Hudson, New York: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 41.0639856°, -73.9204144° Loading map... {"minzoom":false,"mappingservice":"googlemaps3","type":"ROADMAP","zoom":14,"types":["ROADMAP","SATELLITE","HYBRID","TERRAIN"],"geoservice":"google","maxzoom":false,"width":"600px","height":"350px","centre":false,"title":"","label":"","icon":"","visitedicon":"","lines":[],"polygons":[],"circles":[],"rectangles":[],"copycoords":false,"static":false,"wmsoverlay":"","layers":[],"controls":["pan","zoom","type","scale","streetview"],"zoomstyle":"DEFAULT","typestyle":"DEFAULT","autoinfowindows":false,"kml":[],"gkml":[],"fusiontables":[],"resizable":false,"tilt":0,"kmlrezoom":false,"poi":true,"imageoverlays":[],"markercluster":false,"searchmarkers":"","locations":[{"text":"","title":"","link":null,"lat":41.0639856,"lon":-73.9204144,"alt":0,"address":"","icon":"","group":"","inlineLabel":"","visitedicon":""}]}

449

Most Viewed Documents - National Defense | OSTI, US Dept of Energy, Office  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - National Defense Most Viewed Documents - National Defense 2012 Monitoring Research Review: Ground-Based Nuclear Explosion Monitoring Technologies Wetovsky, Marvin A. [Los Alamos National Laboratory]; Anderson, Dale [Los Alamos National Laboratory]; Arrowsmith, Stephen J. [Los Alamos National Laboratory]; et al. (2012) Smart bridge: a tool for estimating the military load classification of bridges Van Groningen, C.N.; Paddock, R.A. (1997) The Effects of Nuclear Weapons Glasstone, Samuel (1964) Detonation and combustion of explosives: A selected bibliography Dobratz, B. [comp.] (1998) SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) Current limiters Loescher, D.H. [Sandia National Labs., Albuquerque, NM (United States).

450

Most Viewed Documents - Engineering | OSTI, US Dept of Energy, Office of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Engineering Most Viewed Documents - Engineering Conduction heat transfer solutions VanSant, J.H. (1980) Lunar Wireless Power Transfer Feasibility Study Sheldon Freid, et al. (2008) Charpy impact test results on five materials and NIST verification specimens using instrumented 2-mm and 8-mm strikers Nanstad, R.K.; Sokolov, M.A. (1995) Heat transfer model of above and underground insulated piping systems Kwon, K.C. (1998) Stress analysis and evaluation of a rectangular pressure vessel. [For equipment for sampling Hanford tank radwaste] Rezvani, M.A.; Ziada, H.H. (Westinghouse Hanford Co., Richland, WA (United States)); Shurrab, M.S. (Westinghouse Savannah River Co., Aiken, SC (United States)) (1992) Damage identification and health monitoring of structural and mechanical systems from changes in their vibration characteristics: A

451

Most Viewed Documents - Fission and Nuclear Technologies | OSTI, US Dept of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Fission and Nuclear Technologies Most Viewed Documents - Fission and Nuclear Technologies Metals design handbook Betts, W.S. (1988) Estimation of gas leak rates through very small orifices and channels. [From sealed PuO/sub 2/ containers under accident conditions] Bomelburg, H.J. () Graphite design handbook Ho, F.H. (1988) Motor-operated valve (MOV) actuator motor and gearbox testing DeWall, K.; Watkins, J.C.; Bramwell, D. [Lockheed Idaho Technologies Co., Idaho Falls, ID (United States)] (1997) Environmental Aspects, Objectives and Targets Identification Process R. Green (2002) Flow-induced vibration of circular cylindrical structures Chen, S.S. (1985) System Definition and Analysis: Power Plant Design and Layout NONE (1996) Materials and design bases issues in ASME Code Case N-47 Huddleston, R.L.; Swindeman, R.W. (Oak Ridge National Lab., TN (United

452

September 2013 Most Viewed Documents for Geosciences | OSTI, US Dept of  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for Geosciences September 2013 Most Viewed Documents for Geosciences Fundamental Research on Percussion Drilling: Improved rock mechanics analysis, advanced simulation technology, and full-scale laboratory investigations Michael S. Bruno (2005) 35 Seismic design evaluation guidelines for buried piping for the DOE HLW Facilities Lin, Chi-Wen [Consultant, Martinez, CA (United States)]; Antaki, G. [Westinghouse Savannah River Co., Aiken, SC (United States)]; Bandyopadhyay, K. [Brookhaven National Lab., Upton, NY (United States)]; Bush, S.H. [Review & Synthesis Association, Richland, WA (United States)]; Costantino, C. [City Univ. of New York, New York, NY (United States)]; Kennedy, R. [RPK Structural Mechanics, Yorba Linda, CA (United States). Consultant] (1995) 29 Proceedings of the wellbore sampling workshop

453

September 2013 Most Viewed Documents for National Defense | OSTI, US Dept  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for National Defense September 2013 Most Viewed Documents for National Defense SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 34 Left-Wing Extremism: The Current Threat Karl A. Seger (2001) 28 The Elusive Coefficients of Thermal Expansion in PBX 9502 C.B. Skidmore; T.A. Butler; C.W. Sandoval (2003) 28 Background chemistry for chemical warfare agents and decontamination processes in support of delisting waste streams at the U.S. Army Dugway Proving Ground, Utah Rosenblatt, D.H.; Small, M.J.; Kimmell, T.A.; Anderson, A.W. (1996) 27 MineSeis -- A MATLAB{reg_sign} GUI program to calculate synthetic seismograms from a linear, multi-shot blast source model Yang, X. (1998) 26 Comments on TNT Equivalence

454

Most Viewed Documents - Materials | OSTI, US Dept of Energy, Office of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Materials Most Viewed Documents - Materials Phase diagrams of the elements Young, D.A. (1975) Use of instrumented charpy tests to determine onset of upper-shelf energy Canonico, D.A.; Stelzman, W.J.; Berggren, R.G.; et al. (1975) Thermal and electrical conductivities and Seebeck coefficients of unirradiated and irradiated graphites from 300 to 1000 K Moore, J.P.; Graves, R.S.; McElroy, D.L. (1973) LITERATURE SURVEY ON DILUTE URANIUM ALLOYS FOR SANDIA BOOSTER CONCEPT TO SANDIA CORPORATION. Fackelmann, J.M.; Bauer, A.A.; Moak, D.P. (1969) Properties of chemical explosives and explosive simulants Dobratz, B.M. (1972) Review of x-ray diffraction studies in uranium alloys Yakel, H.L. (1973) Elevated-temperature true stress-tube strain tensile behavior of AISI Type 304 stainless steel

455

April 2013 Most Viewed Documents for Mathematics And Computing | OSTI, US  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Mathematics And Computing April 2013 Most Viewed Documents for Mathematics And Computing Publications in biomedical and environmental sciences programs, 1981 Moody, J.B. (comp.) (1982) 306 A comparison of risk assessment techniques from qualitative to quantitative Altenbach, T.J. (1995) 159 Lecture notes for introduction to safety and health Biele, F. (1992) 138 Analytical considerations in the code qualification of piping systems Antaki, G.A. (1995) 113 Process Equipment Cost Estimation, Final Report H.P. Loh; Jennifer Lyons; Charles W. White, III (2002) 93 PC-1D installation manual and user's guide Basore, P.A. (1991) 70 SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 69 Computational procedures for determining parameters in

456

April 2013 Most Viewed Documents for Engineering | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Engineering April 2013 Most Viewed Documents for Engineering Charpy impact test results on five materials and NIST verification specimens using instrumented 2-mm and 8-mm strikers Nanstad, R.K.; Sokolov, M.A. (1995) 534 Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 488 Conduction heat transfer solutions VanSant, J.H. (1980) 364 Damage identification and health monitoring of structural and mechanical systems from changes in their vibration characteristics: A literature review Doebling, S.W.; Farrar, C.R.; Prime, M.B.; Shevitz, D.W. (1996) 246 Lunar Wireless Power Transfer Feasibility Study Sheldon Freid, et al. (2008) 229 Heat transfer model of above and underground insulated piping systems Kwon, K.C. (1998) 201 SYNTHETIC SLING FAILURE - EVALUATIONS & RECOMMENDATIONS

457

April 2013 Most Viewed Documents for Physics | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Physics April 2013 Most Viewed Documents for Physics Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; Chou, B.E. (1978) 123 Plastic Gamma Sensors: An Application in Detection of Radioisotopes S. Mukhopadhyay (2003) 85 Organic light emitting diodes (OLEDS) and OLED-based structurally integrated optical sensors Cai, Yuankun (2010) 83 White LED with High Package Extraction Efficiency Yi Zheng; Matthew Stough (2008) 79 Analysis of Lithium-Ion Battery Degradation During Thermal Aging JUNGST,RUDOLPH G.; NAGASUBRAMANIAN,GANESAN; CRAFTS,CHRIS C.; INGERSOLL,DAVID; DOUGHTY,DANIEL H. (2000) 74 Cathodic arc plasma deposition Anders, Andre (2002) 73 Energy level structure and transition probabilities in the spectra of the trivalent lanthanides in LaF/sub 3/. [Tables, diagrams]

458

April 2013 Most Viewed Documents for Geosciences | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Geosciences April 2013 Most Viewed Documents for Geosciences Sloshing analysis of viscous liquid storage tanks Uras, R.Z. (1995) 83 Seismic design evaluation guidelines for buried piping for the DOE HLW Facilities Lin, Chi-Wen [Consultant, Martinez, CA (United States)]; Antaki, G. [Westinghouse Savannah River Co., Aiken, SC (United States)]; Bandyopadhyay, K. [Brookhaven National Lab., Upton, NY (United States)]; Bush, S.H. [Review & Synthesis Association, Richland, WA (United States)]; Costantino, C. [City Univ. of New York, New York, NY (United States)]; Kennedy, R. [RPK Structural Mechanics, Yorba Linda, CA (United States). Consultant] (1995) 48 CO{sub 2}-H{sub 2}O mixtures in the geologic sequestration of CO{sub 2}. I. Assessment and calculation of mutual solubilities from 12 to 100

459

DOE Challenge Home Case Study, Clifton View Homes, Coupeville, WA, Systems Home  

Energy.gov (U.S. Department of Energy (DOE)) Indexed Site

Clifton View Clifton View Homes Coupeville, WA BUILDING TECHNOLOGIES OFFICE DOE Challenge Home builders are in the top 1% of builders in the country meeting the extraordinary levels of excellence and quality specifi ed by the U.S. Department of Energy. Every DOE Challenge Home starts with ENERGY STAR for Homes Version 3 for an energy-effi cient home built on a solid foundation of building science research. Then, even more advanced technologies are designed in for a home that goes above and beyond current code to give you the superior quality construction, HVAC, appliances, indoor air quality, safety, durability, comfort, and solar-ready components along with ultra-low or no utility bills. This provides homeowners with a quality home that will last for generations to come.

460

Basin View Geothermal Heating District, Klamath Falls, Oregon: conceptual design and economic-feasibility study report  

DOE Green Energy (OSTI)

The findings of a feasibility study performed for Basin View Heating District in Klamath Falls, Oregon are reported. The purpose of the study is to determine the physical, economic, and political feasibility of establishing a geothermal heating district to provide space heat to housing units in the Basin View Development of Klamath Falls. Of the several systems considered, all are physically feasible. The project is politically feasible if the owner compiles with governmental requirements. Economic feasibility is based on considerations of money value rates, tax rates and expected rates of return, which are dependent on government and money markets. For analysis a money value rate of 21% and an owner's marginal tax rate of 35% were adopted.

Not Available

1981-07-01T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Questions and Answers - What is the modern view of the structure of the  

NLE Websites -- All DOE Office Websites (Extended Search)

Atoms without neutrons? Atoms without neutrons? Previous Question (Atoms without neutrons?) Questions and Answers Main Index Next Question (Charge of proton and neutron?) Charge of proton and neutron? What is the modern view of the structure of the atom? How are the protons different from neutrons? What are the differences between protons and electrons? There are several concepts that go into our modern view of the atom. I have listed five and they are all "modern" in the sense that they have been developed within the past century. [Concept 1] - Subatomic particles Proton: positive charge, mass is about 2000 times that of the electron Neutron: neutral or zero charge, mass is nearly the same as the proton Electron: negative charge Neutrino: neutral, massless (?) The neutrino is a relatively unknown particle, but plays an important role

462

Most Viewed Documents - Chemistry | OSTI, US Dept of Energy, Office of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Chemistry Most Viewed Documents - Chemistry Flammability characteristics of combustible gases and vapors. [249 refs] Zabetakis, M.G. (1964) Electrical conductivity measurements of aqueous electrolyte solutions at high temperatures and high pressures Ho, P.C.; Palmer, D.A. (1995) Aqueous electrolyte modeling in ASPEN PLUS{trademark} Bloomingburg, G.F. [Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical Engineering]|[Oak Ridge National Lab., TN (United States)]; Simonson, J.M.; Moore, R.C.; et al. (1995) Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) Reaction mechanisms in cellulose pyrolysis: a literature review Molton, P.M.; Demmitt, T.F. () Ultrasonic Sensor and Method of use Condreva, Kenneth J. (1999)

463

Most Viewed Documents - Geosciences | OSTI, US Dept of Energy, Office of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Geosciences Most Viewed Documents - Geosciences Temperature Profiles and Hydrologic Implications from the Nevada Test Site David Gillespie (2005) 3-D full waveform inversion of seismic data; Part I. Theory Lee, Ki Ha (2003) Drilling and coring methods that minimize the disturbance of cuttings, core, and rock formation in the unsaturated zone, Yucca Mountain, Nevada Hammermeister, D.P.; Blout, D.O.; McDaniel, J.C. (1985) CO{sub 2}-H{sub 2}O mixtures in the geologic sequestration of CO{sub 2}. I. Assessment and calculation of mutual solubilities from 12 to 100 degrees C and up to 600 bar Spycher, Nicolas; Pruess, Karsten; Ennis-King, Jonathan (2002) ADVANCED CHARACTERIZATION OF FRACTURED RESERVOIRS IN CARBONATE ROCKS: THE MICHIGAN BASIN James R. Wood; William B. Harrison (2002)

464

September 2013 Most Viewed Documents for Chemistry | OSTI, US Dept of  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for Chemistry September 2013 Most Viewed Documents for Chemistry Molecular catalytic hydrogenation of aromatic hydrocarbons and hydrotreating of coal liquids. Yang, Shiyong; Stock, L.M. (1996) 36 Flammability characteristics of combustible gases and vapors. [249 refs] Zabetakis, M.G. (1964) 35 Reaction mechanisms in cellulose pyrolysis: a literature review Molton, P.M.; Demmitt, T.F. (1977) 32 Nuclear magnetic resonance measurement of ammonia diffusion in dense solid-liquid slurries. Revision 1 Bobroff, S.; Phillips, R.J. [Univ. of California, Davis, CA (United States). Dept. of Chemical Engineering]; Shekarriz, A. [Pacific Northwest National Lab., Richland, WA (United States)] (1998) 30 Determination of NAD+ and NADH level in a Single Cell Under H2O2 Stress by Capillary Electrophoresis

465

April 2013 Most Viewed Documents for Environmental Sciences | OSTI, US Dept  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Environmental Sciences April 2013 Most Viewed Documents for Environmental Sciences Building a secondary containment system Broder, M.F. (1994) 144 Methods and opportunities in the recycling of rare earth based materials Ellis, T.W.; Schmidt, F.A.; Jones, L.L. (1994) 134 Ecological Screening Values for Surface Water, Sediment, and Soil Friday, G. P. (1999) 128 Ammonia usage in vapor compression for refrigeration and air-conditioning in the United States Fairchild, P.D.; Baxter, V.D. (1995) 124 SURVEY OF NOISE SUPPRESSION SYSTEMS FOR ENGINE GENERATOR SETS. KRISHNA,C.R. (1999) 95 Atmospheric Chemistry and Greenhouse Gases Ehhalt, D.; Prather, M.; Dentener, F.; Derwent, R.; Dlugokencky, Edward J.; Holland, E.; Isaksen, I.; Katima, J.; Kirchhoff, V.; Matson, P.; Midgley, P.; Wang, M.; Berntsen, T.; Bey, I.; Brasseur, G.; Buja, L.; Collins, W.

466

September 2013 Most Viewed Documents for Biology And Medicine | OSTI, US  

Office of Scientific and Technical Information (OSTI)

September 2013 Most Viewed Documents for Biology And Medicine September 2013 Most Viewed Documents for Biology And Medicine Drug Retention Times Center for Human Reliability Studies (2007) 29 Oleoresin Capsicum toxicology evaluation and hazard review Archuleta, M.M. (1995) 27 SURVEY OF NOISE SUPPRESSION SYSTEMS FOR ENGINE GENERATOR SETS. KRISHNA,C.R. (1999) 27 Site-Directed Research and Development FY 2012 Annual Report , (2013) 27 Human radiation studies: Remembering the early years. Oral history of Donner Lab Administrator Baird G. Whaley, August 15, 1994 NONE (1995) 26 Degradation of high concentrations of glycols, antifreeze, and deicing fluids Strong-Gunderson, J.M.; Wheelis, S.; Carroll, S.L.; Waltz, M.D.; Palumbo, A.V. (1995) 24 Human radiation studies: Remembering the early years. Oral history of Dr. George Voelz, M.D., November 29, 1994

467

April 2013 Most Viewed Documents for National Defense | OSTI, US Dept of  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for National Defense April 2013 Most Viewed Documents for National Defense Vapor-liquid equilibria for nitric acid-water and plutonium nitrate-nitric acid-water solutions Maimoni, A. (1980) 95 SMART BRIDGE: A tool for estimating the military load classification of bridges using varying levels of information Van Groningen, C.N.; Paddock, R.A. (1997) 69 LLNL small-scale drop-hammer impact sensitivity test Simpson, L.R.; Foltz, M.F. (1995) 67 Comments on TNT Equivalence Cooper, P.W. (1994) 66 Background chemistry for chemical warfare agents and decontamination processes in support of delisting waste streams at the U.S. Army Dugway Proving Ground, Utah Rosenblatt, D.H.; Small, M.J.; Kimmell, T.A.; Anderson, A.W. (1996) 65 Weapon container catalog. Volumes 1 & 2 Brown, L.A.; Higuera, M.C. (1998)

468

April 2013 Most Viewed Documents for Materials | OSTI, US Dept of Energy,  

Office of Scientific and Technical Information (OSTI)

April 2013 Most Viewed Documents for Materials April 2013 Most Viewed Documents for Materials Fatigue design curves for 6061-T6 aluminum Yahr, G.T. (1993) 554 Charpy impact test results on five materials and NIST verification specimens using instrumented 2-mm and 8-mm strikers Nanstad, R.K.; Sokolov, M.A. (1995) 534 Heat Treatment Procedure Qualification -- Final Technical Report Robert C. Voigt (2004) 488 The influence of grain size on the mechanical properties ofsteel Morris Jr., J.W. (2001) 356 Damage identification and health monitoring of structural and mechanical systems from changes in their vibration characteristics: A literature review Doebling, S.W.; Farrar, C.R.; Prime, M.B.; Shevitz, D.W. (1996) 246 Graphite design handbook Ho, F.H. (1988) 216 Mechanical properties and energy absorption characteristics of a

469

Most Viewed Documents - Physics | OSTI, US Dept of Energy, Office of  

Office of Scientific and Technical Information (OSTI)

Most Viewed Documents - Physics Most Viewed Documents - Physics Computational procedures for determining parameters in Ramberg-Osgood elastoplastic model based on modulus and damping versus strain Ueng, Tzou-Shin; Chen, Jian-Chu. (1992) Analysis of Lithium-Ion Battery Degradation During Thermal Aging JUNGST,RUDOLPH G.; NAGASUBRAMANIAN,GANESAN; CRAFTS,CHRIS C.; et al. (2000) Plastic Gamma Sensors: An Application in Detection of Radioisotopes S. Mukhopadhyay (2003) Lithium literature review: lithium's properties and interactions Jeppson, D.W.; Ballif, J.L.; Yuan, W.W.; et al. (1978) Fire protection system operating experience review for fusion applications Cadwallader, L.C. (1995) Organic light emitting diodes (OLEDS) and OLED-based structurally integrated optical sensors Cai, Yuankun (2010)

470

The plant mitochondrial mat-r gene/nad1 gene complex. Progress report  

DOE Green Energy (OSTI)

The authors have completed sequencing the segments (totalling 19 kb, both complementary strands) of the maize mtDNA molecule that encode the entire NADH dehydrogenase subunit (nadl) gene. They have identified nucleotides in mature transcripts of the nadl gene that are edited and have generated clones of cDNAs of entire mature (fully spliced) nadl transcripts. They have examined the relative rates of splicing in transcripts of the four nadl gene group II introns and begun examining nadl intron cDNAs to determine the extent and distribution of RNA edits in introns, in order to evaluate the possibility that intron excision and exon splicing might be editing independent.

Wolstenholme, D.R.

1994-06-01T23:59:59.000Z

471

Field Guide: Visual Inspection of Steel Structures (Optimized for Electronic Viewing)  

Science Conference Proceedings (OSTI)

This EPRI report, one in a series of practical guides designed as reference aids for utility personnel working in the field, visually catalogs the various condition issues that commonly affect transmission line steel structures. The scope includes steel poles, lattice structures, connections, foundations, weathering steel, and coatings.This field guide has been optimized for viewing on electronic devices. For a copy of this product printed on high-quality paper and ring-bound in a ...

2011-12-09T23:59:59.000Z

472

Field Guide: Inspection of Transmission Line Grounding Systems (Optimized for Electronic Viewing)  

Science Conference Proceedings (OSTI)

This EPRI report, one in a series of practical guides designed as reference aids for utility personnel working in the field, visually catalogs the various condition issues that commonly affect transmission line grounding systems. It presents photographs and short written descriptions of the conditions and lists associated causes, failure modes, and impacts. This field guide has been optimized for viewing on electronic devices. For a standard PDF or copy of this product printed on high-quality paper ...

2011-12-09T23:59:59.000Z

473

Field Guide: Transmission Line Aerial Marking and Lighting (Optimized for Electronic Viewing)  

Science Conference Proceedings (OSTI)

Various types of aerial marking and lighting are applied to transmission structures and wires as a safety enhancement to improve the visibility of structures and lines. This EPRI report, one in a series of practical guides designed as reference aids for utility personnel working in the field, is devoted to the subject of transmission line aerial marking and lighting, with an emphasis on inspection.This field guide has been optimized for viewing on electronic devices. For a standard PDF ...

2011-12-09T23:59:59.000Z

474

PlantView Alarm Response Module 2.119.001 User Manual  

Science Conference Proceedings (OSTI)

PlantView's174Alarm Response Database provides on-line support of alarm knowledge in support of operations or as a source of training references. Searching a system from the navigation tree initiates a pop-up with the alarm data. Including cues for validation and corresponding control loop to ensure automatic operations. As a troubleshooting aid Alarm Response provides identification of probable causes, recommended corrective action procedures, possible overreaching effects to the system operation, and a...

2011-10-04T23:59:59.000Z

475

Integrating gene expression profiling and clinical data  

Science Conference Proceedings (OSTI)

We propose a combination of machine learning techniques to integrate predictive profiling from gene expression with clinical and epidemiological data. Starting from BioDCV, a complete software setup for predictive classification and feature ranking without ... Keywords: BioDCV, Biomarkers, Classification, DNA microarray, Feature selection, Functional genomics, SVM, Statistical learning

Silvano Paoli; Giuseppe Jurman; Davide Albanese; Stefano Merler; Cesare Furlanello

2008-01-01T23:59:59.000Z

476

Information Retrieval meets Gene Analysis Hagit Shatkay  

E-Print Network (OSTI)

Information Retrieval meets Gene Analysis Hagit Shatkay Celera Genomics 45 West Gude Drive, based on probabilistic information retrieval, which uses the lit- erature to establish functional-related publications. This wealth of information presents a major data-analysis challenge. An ultimate goal is to under

Shatkay, Hagit

477

Mechanisms of radiation-induced gene responses  

Science Conference Proceedings (OSTI)

In the process of identifying genes differentially expressed in cells exposed ultraviolet radiation, we have identified a transcript having a 26-bp region that is highly conserved in a variety of species including Bacillus circulans, yeast, pumpkin, Drosophila, mouse, and man. When the 5` region (flanking region or UTR) of a gene, the sequence is predominantly in +/+ orientation with respect to the coding DNA strand; while in the coding region and the 3` region (UTR), the sequence is most frequently in the +/-orientation with respect to the coding DNA strand. In two genes, the element is split into two parts; however, in most cases, it is found only once but with a minimum of 11 consecutive nucleotides precisely depicting the original sequence. The element is found in a large number of different genes with diverse functions (from human ras p21 to B. circulans chitonase). Gel shift assays demonstrated the presence of a protein in HeLa cell extracts that binds to the sense and antisense single-stranded consensus oligomers, as well as to the double- stranded oligonucleotide. When double-stranded oligomer was used, the size shift demonstrated as additional protein-oligomer complex larger than the one bound to either sense or antisense single-stranded consensus oligomers alone. It is speculated either that this element binds to protein(s) important in maintaining DNA is a single-stranded orientation for transcription or, alternatively that this element is important in the transcription-coupled DNA repair process.

Woloschak, G.E.; Paunesku, T.

1996-10-01T23:59:59.000Z

478

Molecular Cell Control of Proinflammatory Gene  

E-Print Network (OSTI)

-depen- dent erasure of H4K20me3 is required for effective gene activation and is achieved by NF-kB-depen- dent such as nuclear factor-k B (NF-kB), activator protein 1 (AP1), and interferon regulatory factor (IRFs

Higgins, Darren

479

Book Review Bayesian Inference for Gene Expression  

E-Print Network (OSTI)

Book Review Bayesian Inference for Gene Expression and Proteomics. Edited by Kim-Anh Do, Peter Mu for a long time. This book is a timely publication entirely devoted to cutting-edge Bayesian methods in their own biological research. Moreover, the book calls for more methodological and theoretical research

Vannucci, Marina

480

Gene encoding acetyl-coenzyme A carboxylase  

DOE Patents (OSTI)

A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives are disclosed which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides. 5 figs.

Roessler, P.G.; Ohlrogge, J.B.

1996-09-24T23:59:59.000Z

Note: This page contains sample records for the topic "gene transfection view" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

RAS Gene Hot-Spot Mutations in  

E-Print Network (OSTI)

Point mutations in the cellular homologues HRAS, KRAS2, and NRAS of the viral Harvey and Kirsten rat sarcoma virus oncogenes are commonly involved in the onset of malignancies in humans and other species such as dog, mouse, and rat. Most often, three particular hot-spot codons are affected, with one amino acid exchange being sufficient for the induction of tumor growth. While RAS genes have been shown to play an important role in canine tumors such as non-small lung cell carcinomas, data about RAS mutations in canine fibrosarcomas as well as KRAS2 mutations in canine melanomas is sparse. To increase the number of tumors examined, we recently screened 13 canine fibrosarcomas and 11 canine melanomas for point mutations, particularly within the mutational hot spots. The results were compared to the already existing data from other studies about these tumors in dogs. A family of genes often involved in human tumors are the well-characterized RAS genes, which comprise HRAS, KRAS2, and NRAS, coding for closely related, small, 189 amino acid, 21 kDa, membrane-bound, intracellular proteins. The human cellular HRAS and KRAS2 genes were identified to be homologues of the Harvey and Kirsten rat sarcoma

Canine Neoplasias; J. Bullerdiek

2005-01-01T23:59:59.000Z

482

Assessment of industry views on international business prospects for solar thermal technology  

DOE Green Energy (OSTI)

This report contains a review of solar thermal industry viewpoints on their prospects for developing international business. The report documents the industry's current involvement in foreign markets, view of foreign competition in overseas applications, and view of federal R and D and policy requirements to strengthen international business prospects. The report is based on discussions with equipment manufacturers and system integrators who have a product or service with potential international demand. Interviews with manufacturers and system integrators were conducted by using a standard format for interview questions. The use of a standard format for questions provided a basis for aggregating similar views expressed by US companies concerning overseas business prospects. A special effort was made to gather responses from the entire solar thermal industry, including manufacturers of line-focus, point-focus, and central receiver systems. General, technical, economic, institutional, and financial findings are provided in this summary. In addition, Pacific Northwest Laboratory (PNL) recommendations are provided (based upon advice from the Solar Thermal Review Panel) for activities to improve US solar thermal business prospects overseas.

Easterling, J.C.

1984-09-01T23:59:59.000Z

483

Interactive Computer-Enhanced Remote Viewing System (ICERVS): Final report, November 1994--September 1996  

Science Conference Proceedings (OSTI)

The Interactive Computer-Enhanced Remote Viewing System (ICERVS) is a software tool for complex three-dimensional (3-D) visualization and modeling. Its primary purpose is to facilitate the use of robotic and telerobotic systems in remote and/or hazardous environments, where spatial information is provided by 3-D mapping sensors. ICERVS provides a robust, interactive system for viewing sensor data in 3-D and combines this with interactive geometric modeling capabilities that allow an operator to construct CAD models to match the remote environment. Part I of this report traces the development of ICERVS through three evolutionary phases: (1) development of first-generation software to render orthogonal view displays and wireframe models; (2) expansion of this software to include interactive viewpoint control, surface-shaded graphics, material (scalar and nonscalar) property data, cut/slice planes, color and visibility mapping, and generalized object models; (3) demonstration of ICERVS as a tool for the remediation of underground storage tanks (USTs) and the dismantlement of contaminated processing facilities. Part II of this report details the software design of ICERVS, with particular emphasis on its object-oriented architecture and user interface.

NONE

1997-05-01T23:59:59.000Z

484

Abnormal Gene Expression of Four Genes in Cells from Family Members of  

NLE Websites -- All DOE Office Websites (Extended Search)

Abnormal Gene Expression of Four Genes in Cells from Family Members of Abnormal Gene Expression of Four Genes in Cells from Family Members of Hereditary-type Retinoblastoma Patients relative to Normal Individuals Chin-Yu Wang, 1 Yuanlin Peng, 1 Zhonghui Yang, 2 Chuan-Yuan Li, 2 Hatsumi Nagasawa, 1 Markus M. Fitzek, 3 John B. Little, 4 Joel S. Bedford, 1 and Eric Y. Chuang 5 1 Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado; 2 Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina; 3 Department of Radiation Oncology, Tufts-New England Medical Center; 4 Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts; and 5 Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan

485

Caenorhabditis elegans aristaless/Arx gene alr-1 restricts variable gene expression  

E-Print Network (OSTI)

Variable expressivity of mutant phenotypes in genetically identical individuals is a phenomenon widely reported but poorly understood. For example, mutations in the gene encoding the transcription factor ALR-1 in Caenorhabditis ...

Topalidou, Irini

486

Gene family evolution by duplication, speciation and loss - CECM  

E-Print Network (OSTI)

increase of the lower bound. Let S = {S1,...,Sk} be a set .... (expected number of event by million years) and a gene loss rate of 0.02. We chose a gene gain/loss ...

487

Account Request Form | IBM Blue Gene Parallel Supercomputer,...  

NLE Websites -- All DOE Office Websites (Extended Search)

(check all that apply) Please make at least one selection Brookhaven Blue GeneQ Argonne Leadership Computing Facility (based on Blue GeneQ) What is the name of the code? Do you...

488

Mediator and cohesin connect gene expression and chromatin architecture  

E-Print Network (OSTI)

Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound ...

Kagey, Michael H.

489

Distal chromatin structure influences local nucleosome positions and gene expression  

E-Print Network (OSTI)

The positions of nucleosomes across the genome influence several cellular processes, including gene transcription. However, our understanding of the factors dictating where nucleosomes are located and how this affects gene ...

Jansen, An

490

Cross-regulation and interaction between eukaryotic gene regulatory processes  

E-Print Network (OSTI)

Regulation of genes is fundamental to all living processes and can be exerted at many sequential steps. We studied several eukaryotic gene regulatory mechanisms with an emphasis on understanding the interplay between ...

Spies, Noah (Noah Walter Benjamin)

2012-01-01T23:59:59.000Z

491

Performance Analysis of Enhanced Clustering Algorithm for Gene Expression Data  

E-Print Network (OSTI)

Microarrays are made it possible to simultaneously monitor the expression profiles of thousands of genes under various experimental conditions. It is used to identify the co-expressed genes in specific cells or tissues that are actively used to make proteins. This method is used to analysis the gene expression, an important task in bioinformatics research. Cluster analysis of gene expression data has proved to be a useful tool for identifying co-expressed genes, biologically relevant groupings of genes and samples. In this paper we applied K-Means with Automatic Generations of Merge Factor for ISODATA- AGMFI. Though AGMFI has been applied for clustering of Gene Expression Data, this proposed Enhanced Automatic Generations of Merge Factor for ISODATA- EAGMFI Algorithms overcome the drawbacks of AGMFI in terms of specifying the optimal number of clusters and initialization of good cluster centroids. Experimental results on Gene Expression Data show that the proposed EAGMFI algorithms could identify compact clus...

Chandrasekhar, T; Elayaraja, E

2011-01-01T23:59:59.000Z

492

Casein genes of Bos taurus. II. Isolation and characterization of the /beta/-casein gene  

SciTech Connect

The expression of the casein genes in the cells of the mammary gland is regulated by peptide and steroid hormones. In order to study the controlling mechanisms we have isolated and characterized the /beta/-casein gene. The gene is 8.6 kb long and exceeds by a factor of 7.8 the length of the corresponding mRNA which is encoded by nine exons. The genomic clones incorporate in addition 8.5 kb and 4.5 kb of the 5/prime/- and 3/prime/-flanking regions. We have determined the sequence of the 5- and 3-terminals of the gene and have performed a comparative analysis of the corresponding regions of the rat /beta/-casein gene. Furthermore we have identified the conversed sequences identical or homologous to the potential sections of binding to the nuclear factor CTF/NF-1 by glucocorticoid and progesterone receptors. The regulatory region of the bovine casein gene contains two variants of the TATA signal, flanking the duplication section in the promoter region.

Gorodetskii, S.I.; Tkach, T.M.; Kapelinskaya, T.V.

1988-11-01T23:59:59.000Z

493

Ataxia-telangiectasia: mutations in the ATM gene  

DOE Patents (OSTI)

The invention is related to ataxia-telangiectasia, specifically, mutations in the ataxia-telangiectasia mutated gene.

Gatti, Richard A. (3835 Longridge Ave., Sherman Oaks, CA 91243); Concannon, Patrick J. (5335 old Mill Rd. NE., Bainbridge Island, WA 98110)

1999-01-01T23:59:59.000Z

494

Functional visualisation of genes using singular value decomposition  

Science Conference Proceedings (OSTI)

Progress in understanding core pathways and processes of cancer requires thorough analysis of many coding regions of the genome. New insights are hampered due to the lack of tools to make sense of large lists of genes identified using high throughput ... Keywords: gene ontology, genes, singular value decomposition, visualisation

Hamid Ghous, Paul J. Kennedy, Nicholas Ho, Daniel R. Catchpoole

2012-12-01T23:59:59.000Z

495

Parametric spectral analysis of malaria gene expression time series data  

Science Conference Proceedings (OSTI)

Spectral analysis of DNA microarray gene expressions time series data is important for understanding the regulation of gene expression and gene function of the Plasmodium falciparum in the intraerythrocytic developmental cycle. In this paper, ... Keywords: autoregressive model, microarray time series analysis, plasmodium falciparum, singular spectrum analysis, spectral estimation

Liping Du; Shuanhu Wu; Alan Wee-Chung Liew; David Keith Smith; Hong Yan

2006-09-01T23:59:59.000Z

496

Classification of co-expressed genes from DNA regulatory regions  

Science Conference Proceedings (OSTI)

The analysis of non-coding DNA regulatory regions is one of the most challenging open problems in computational biology. In this paper we investigate whether we can predict functional information about genes by using information extracted from their ... Keywords: Combinatorial and machine learning methods integration, Gene classification, Gene expression and bio-sequence data integration, Motif extraction and selection

Giulio Pavesi; Giorgio Valentini

2009-07-01T23:59:59.000Z

497

Synthetic gene design with a large number of hidden stops  

Science Conference Proceedings (OSTI)

Hidden stops are nucleotide triples TAA, TAG and TGA that appear on the second and third reading frames of a protein coding gene. Recent studies suggested the important role of hidden stops in preventing misread of mRNA. We study the problem of designing ... Keywords: back translation, bioinformatics, codons, gene design, hidden stops, mRNA, protein coding genes, synthetic biology

Vinhthuy Phan; Sudip Saha; Ashutosh Pandey; Tit-Yee Wong

2010-07-01T23:59:59.000Z

498

The ribosomal protein genes and Minuteloci of Drosophila melanogaster  

E-Print Network (OSTI)

necessary, D. mela- nogaster genes were named or renamed according to the standard metazoan RP gene nomenclature proposed by Wool and colleagues [5,58,59] and approved by the HUGO Gene Nomenclature Committee [18], whilst still conforming to Fly- Base [60...

Marygold, Steven J; Roote, John; Reuter, Gunter; Lambertsson, Andrew; Ashburner, Michael; Millburn, Gillian; Harrison, Paul; Yu, Zhan; Kenmochi, Naoya; Kaufman, Thomas C; Leevers, Sally J; Cook, Kevin R

2007-10-10T23:59:59.000Z

499

Wind Energy Facilities and Residential Properties: The Effect of Proximity and View on Sales Prices  

SciTech Connect

With an increasing number of communities considering nearby wind power developments, there is a need to empirically investigate community concerns about wind project development. One such concern is that property values may be adversely affected by wind energy facilities, and relatively little research exists on the subject. The present research investigates roughly 7,500 sales of single-family homes surrounding 24 existing U.S. wind facilities. Across four different hedonic models the results are consistent: neither the view of the wind facilities nor the distance of the home to those facilities is found to have a statistically significant effect on home sales prices.

Hoen, Ben; Wiser, Ryan; Cappers, Peter; Thayer, Mark; Sethi, Gautam

2010-04-01T23:59:59.000Z

500

Biomass stakeholder views and concerns: Environmental groups and some trade association  

DOE Green Energy (OSTI)

This exploratory study of the views and concerns of 25 environmental organizations found high interest and concern about which biomass feedstocks would be used and how these biomass materials would be converted to energy. While all favored renewable energy over fossil or nuclear energy, opinion diverged over whether energy crops, residues, or both should be the primary source of a biomass/bioenergy fuel cycle. About half of the discussants favored biomass ``in general'' as a renewable energy source, while the others were distributed about equally over five categories, from favor-with-conditions, uncertain, skeptical, opposed, to ``no organizational policy.''

Peelle, E.

2000-01-01T23:59:59.000Z