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Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

Covered Product Category: Compact Fluorescent Lamps  

Broader source: Energy.gov [DOE]

FEMP provides acquisition guidance across a variety of product categories, including compact fluorescent lamps (CFLs), which are an ENERGY STAR-qualified product category.

2

Thermally efficient compact fluorescent fixture systems  

SciTech Connect (OSTI)

Compact fluorescent lamps that can be inserted into conventional light fixtures are rapidly gaining acceptance as both a viable retrofit and new design approach to reducing lighting loads. Ideally, the compact fluorescent lamp should have the same light output as the incandescent lamp it replaces, but overheating inside typically small enclosed fixtures can reduce lumen output and hence lighting fixture efficiency by 15 to 20 percent. Fortunately, simple fixture modifications can erase this efficiency penalty, so that the full efficiency benefit of replacing incandescent lamps with fluorescent lamps can be realized. The paper describes such modifications and presents experimental data documenting the potential efficiency enhancement associated with thermal control systems. 4 refs., 7 figs.

Siminovitch, M.J.; Rubinstein, F.M.; Packer, M.

1991-04-01T23:59:59.000Z

3

Compact fluorescent lamp applications in luxury hotels  

SciTech Connect (OSTI)

Over the past several years, consumers, lighting designers, and energy conservationists have paid increasing attention to the special characteristics of compact fluorescent lamps (CFLs). CFLs can typically be used to replace incandescent lamps of three to four times their own wattage, and their color rendering indices (CRIs)-80 to 85-make them virtually indistinguishable from incandescents. The typical 10,0000-hour life of a CFL often makes savings in labor its most desirable feature when compared to a shorter-lived incandescent lamp.

Gilleskie, R.J.

1996-01-01T23:59:59.000Z

4

Perceptions of compact fluorescent lamps in the residential market  

SciTech Connect (OSTI)

Compact fluorescent lamps offer significant energy savings over other forms of residential lighting and last up to 10 times longer than conventional incandescent bulbs. In order to better understand existing barriers to acceptance and future opportunities for growth of compact fluorescent lighting in the residential retrofit sector, a three stage research project was designed and conducted by MACRO Consulting, Inc. Assessment of whether or not the benefits of compact fluorescent lamps are sufficient to overcome price resistance was one of the major purposes of this project. Residential customers were interviewed in focus group sessions to help determine key issues and motivating forces in the lighting/energy saving/cost saving equation. Residential customers in 5 major market areas were contacted by telephone, and data about their awareness, knowledge and use of compact fluorescent lighting were collected. These customers also participated in an attribute rating exercise in which compact fluorescent lamps were compared with fluorescent tubes and incandescent bulbs on a series of product attributes. A price elasticity exercise was also conducted. Teleconferences with retailers of compact fluorescent lamps were conducted in order to explore their knowledge of and attitudes towards compact fluorescent lamps. Customers agree that energy savings and longer life are both positive attributes for residential lighting products, but they are not yet ready to make the switch away from inexpensive, versatile and readily available incandescent bulbs to compact fluorescent lamps. Compact fluorescent lamps are rated poorly (even by satisfied'' users) on each of seven positive attributes of home lighting. Major barriers to increased use of compact fluorescent lamps include price, convenience, and performance. Prices above $10 are considered outrageous''. Product improvements are needed for appearance, light output and versatility.

Weiner, J.; Campbell, C.J. (Macro Consulting, Inc., Mountain View, CA (United States))

1992-07-01T23:59:59.000Z

5

Lighting the Way with Compact Fluorescent Lighting | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Lighting the Way with Compact Fluorescent Lighting Lighting the Way with Compact Fluorescent Lighting Lighting the Way with Compact Fluorescent Lighting April 28, 2009 - 5:00am Addthis John Lippert There is a major push today to get homeowners to adopt compact fluorescent lamp (CFL) light bulbs. They have been on the market for nearly three decades, and many homeowners still do not use them widely. But the tide is definitely turning. Their availability and the percentage of homeowners familiar with the technology and purchasing them for their homes have been steadily rising. The products have improved considerably compared to early products, and their prices have plummeted. The ENERGY STAR® Change a Light, Change the World Campaign has been running now for more than half a dozen years. This campaign is designed to

6

Covered Product Category: Compact Fluorescent Lamps | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Covered Product Category: Compact Fluorescent Lamps Covered Product Category: Compact Fluorescent Lamps Covered Product Category: Compact Fluorescent Lamps October 7, 2013 - 10:48am Addthis ENERGY STAR Qualified Products Federal Energy Management Program (FEMP) provides acquisition guidance across a variety of product categories, including compact fluorescent lamps (CFLs), which are an ENERGY STAR-qualified product category. Federal laws and executive orders mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law. Most manufacturers display the ENERGY STAR label on complying models. For a model not displaying this label, check the manufacturer's literature to determine if it meets the efficiency requirements outlined by ENERGY STAR.

7

Lighting the Way with Compact Fluorescent Lighting | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Lighting the Way with Compact Fluorescent Lighting Lighting the Way with Compact Fluorescent Lighting Lighting the Way with Compact Fluorescent Lighting April 28, 2009 - 5:00am Addthis John Lippert There is a major push today to get homeowners to adopt compact fluorescent lamp (CFL) light bulbs. They have been on the market for nearly three decades, and many homeowners still do not use them widely. But the tide is definitely turning. Their availability and the percentage of homeowners familiar with the technology and purchasing them for their homes have been steadily rising. The products have improved considerably compared to early products, and their prices have plummeted. The ENERGY STAR® Change a Light, Change the World Campaign has been running now for more than half a dozen years. This campaign is designed to

8

Energy Cost Calculator for Compact Fluorescent Lamps | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Compact Fluorescent Lamps Compact Fluorescent Lamps Energy Cost Calculator for Compact Fluorescent Lamps October 8, 2013 - 2:18pm Addthis This tool calculates the payback period for your calc retrofit project. Modify the default values to suit your project requirements. Existing incandescent lamp wattage Watts Incandescent lamp cost dollars Incandescent lamp life 1000 hours calc wattage Watts calc cost dollars calc life (6000 hours for moderate use, 10000 hours for high use) 8000 hours Number of lamps in retrofit project Hours operating per week hours Average cost of electricity 0.06 $/kWh Relamper labor costs $/hr Time taken to retrofit all lamps in this project min Time taken to relamp one lamp min Type of Relamping Practiced: Group Relamping: Calculate Simple Payback Period months

9

Mercury Lamps Recycling Fluorescent light-tubes, compact fluorescent bulbs, mercury and sodium vapor lamps, ultraviolet and  

E-Print Network [OSTI]

Mercury Lamps Recycling Fluorescent light-tubes, compact fluorescent bulbs, mercury and sodium vapor lamps, ultraviolet and HID (high-intensity discharge) lamps and all other mercury containing labeled for shipment to a recycling plant for mercury, glass and aluminum recovery. The beneficial re

Baker, Chris I.

10

Medium Base Compact Fluorescent Lamps | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Templates for Reporting Test Results mediumbasecfltestreporttemplatev1.1.xlsx More Documents & Publications Fluorescent Lamp Ballasts Showerheads Beverage Vending Machines...

11

DOE Withdraws the Energy Star Label from 34 Compact Fluorescent Light Bulbs  

Broader source: Energy.gov (indexed) [DOE]

the Energy Star Label from 34 Compact Fluorescent the Energy Star Label from 34 Compact Fluorescent Light Bulbs DOE Withdraws the Energy Star Label from 34 Compact Fluorescent Light Bulbs January 26, 2010 - 11:41am Addthis Washington, DC - On January 25th, the General Counsel notified 25 manufacturers that the Department of Energy has withdrawn their right to use the Energy Star label on 34 different models of compact fluorescent light bulbs (CFLs). The Department took this action after its off-the-shelf testing revealed that the affected models do not last as long in regular use as Energy Star certification would require. As a result, these manufacturers have been informed that they can no longer ship or sell any of the 34 models of CFLs bearing the Energy Star label on the bulb or its packaging. All compact fluorescent light bulbs use about 75 percent less energy and

12

The effects of supply harmonics on the performance of compact fluorescent lamps  

SciTech Connect (OSTI)

This paper describes a performance evaluation of ten compact fluorescent lighting systems operated with sinusoidal and distorted voltage waveform conditions. The lamps were either provided with an electronic ballast, a magnetic core ballast or a magnetic core ballast with a power factor correction circuit. The test results show that the electrical performance of the compact fluorescent lamps for both sinusoidal and distorted voltage waveform operation is related to the different types of ballast used. The cost of operation of these compact fluorescent lamps was calculated and compared to the cost of a conventional 60 W incandescent lamp.

Arseneau, R.; Ouellette, M.

1993-04-01T23:59:59.000Z

13

Compact Fluorescent Plug-In Ballast-in-a-Socket  

SciTech Connect (OSTI)

The primary goal of this program was to develop a ballast system for plug-in CFLs (compact fluorescent lamps) that will directly replace standard metal shell, medium base incandescent lampholders (such as Levition No. 6098) for use with portable lamp fixtures, such as floor, table and desk lamps. A secondary goal was to identify a plug-in CFL that is optimized for use with this ballast. This Plug-in CFL Ballastin-a-Socket system will allow fixture manufacturers to easily manufacture CFL-based high-efficacy portable fixtures that provide residential and commercial consumers with attractive, cost-effective, and energy-efficient fixtures for use wherever portable incandescent fixtures are used today. The advantages of this proposed system over existing CFL solutions are that the fixtures can only be used with high-efficacy CFLs, and they will be more attractive and will have lower life-cycle costs than screw-in or adapter-based CFL retrofit solutions. These features should greatly increase the penetration of CFL's into the North American market. Our work has shown that using integrated circuits it is quite feasible to produce a lamp-fixture ballast of a size comparable to the current Edison-screw 3-way incandescent fixtures. As for price points for BIAS-based fixtures, end-users polled by the Lighting Research Institute at RPI indicated that they would pay as much as an additional $10 for a lamp containing such a ballast. The ballast has been optimized to run with a 26 W amalgam triple biax lamp in the base-down position, yet can accept non-amalgam versions of the lamp. With a few part alterations, the ballast can be produced to support 32 W lamps as well. The ballast uses GE's existing L-Comp[1] power topology in the circuit so that the integrated circuit design would be a design that could possibly be used by other CFL and EFL products with minor modifications. This gives added value by reducing cost and size of not only the BIAS, but also possibly other integral CFL and future dimmable integral and plug-in versions of the EFL products.

Rebecca Voelker

2001-12-21T23:59:59.000Z

14

Frequently Asked Questions Information on Compact Fluorescent Light Bulbs (CFLs) and Mercury  

E-Print Network [OSTI]

Frequently Asked Questions Information on Compact Fluorescent Light Bulbs (CFLs) and Mercury emissions equivalent to those of more than 800,000 cars. Do CFLs contain mercury? CFLs contain a very small amount of mercury sealed within the glass tubing ­ an average of 5 milligrams ­ about the amount

Jia, Songtao

15

Compact fluorescent lamp using horizontal and vertical insulating septums and convective venting geometry  

DOE Patents [OSTI]

A novel design is described for a compact fluorescent lamp, including a lamp geometry which will increase light output and efficacy of the lamp in a base down operating position by providing horizontal and vertical insulating septums positioned in the ballast compartment of the lamp to provide a cooler coldspot. Selective convective venting provides additional cooling of the ballast compartment. 9 figs.

Siminovitch, M.

1998-02-10T23:59:59.000Z

16

Mercury Vapor Release from Broken Compact Fluorescent Lamps and In Situ Capture by New Nanomaterial Sorbents  

Science Journals Connector (OSTI)

There is one report of Hg poisoning (acrodynia) in a child exposed to broken tube-type fluorescents in a detailed case study presented by Tunnessen et al. (6). ... Similar release patterns but lower amounts were seen for spent bulbs (example result 90 ?g in 24 h) or from the fracture site of a new bulb after glass removal to simulate cleanup. ... Artificial lighting systems are transitioning from incandescent to compact fluorescent lamp (CFL) and light-emitting diode (LED) bulbs in response to the U.S. Energy Independence and Security Act and the EU Ecodesign Directive, which leads to energy ... ...

Natalie C. Johnson; Shawn Manchester; Love Sarin; Yuming Gao; Indrek Kulaots; Robert H. Hurt

2008-06-27T23:59:59.000Z

17

Compact Fluorescent Lighting in America: Lessons Learned on the Way to Market  

SciTech Connect (OSTI)

This report describes the history of compact fluorescent lamps (CFLs) in America. CFLs were introduced in the 1970s; however, it has taken more than 20 years for them to gain widespread recognition in the U.S. residential lighting market. This report reviews the development of CFLs, efforts to increase market acceptance of them, and barriers to that acceptance. Lessons to be learned from this study of CFLs are identified in hopes of assisting future market introduction efforts for other promising energy-efficient technologies. This report was prepared by the Pacific Northwest National Laboratory for the U.S. Department of Energys Office of Building Technologies, Emerging Technologies Program.

Sandahl, Linda J.; Gilbride, Theresa L.; Ledbetter, Marc R.; Steward, Heidi E.; Calwell, Chris

2006-05-22T23:59:59.000Z

18

Fluorescent Lighting | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Fluorescent Lighting Fluorescent Lighting Fluorescent Lighting October 17, 2013 - 5:44pm Addthis Fluorescent Lighting Fluorescent Lighting Fluorescent lamps use 25%-35% of the energy used by incandescent products to provide a similar amount of light. They also last about 10 times longer (7,000-24,000 hours). The two general types of fluorescent lamps are: Compact fluorescent lamps (CFLs) -- commonly found with integral ballasts and screw bases, these are popular lamps often used in household fixtures Fluorescent tube and circline lamps -- typically used for task lighting such as garages and under cabinet fixtures, and for lighting large areas in commercial buildings. CFLs CFLs combine the energy efficiency of fluorescent lighting with the convenience and popularity of incandescent fixtures. CFLs fit most fixtures

19

,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen"  

U.S. Energy Information Administration (EIA) Indexed Site

B39. Lighting Equipment, Floorspace, 1999" B39. Lighting Equipment, Floorspace, 1999" ,"Total Floorspace (million square feet)" ,"All Buildings","All Lit Buildings","Lighting Equipment (more than one may apply)" ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen" "All Buildings ................",67338,64321,38156,60344,20666,19223,17926 "Building Floorspace" "(Square Feet)" "1,001 to 5,000 ...............",6774,5859,2946,5154,738,245,600 "5,001 to 10,000 ..............",8238,7464,4047,6722,1108,663,991 "10,001 to 25,000 .............",11153,10393,6055,9815,1759,1701,1996 "25,001 to 50,000 .............",9311,9053,5004,8344,2296,2224,1611

20

Energy-efficient compact screw-in fluorescent lamp. Final report  

SciTech Connect (OSTI)

A compact fluorescent lamp has been designed and constructed which can replace an incandescent lamp. The lamp is slightly larger than a standard lamp (8 3/4 in. x 3 1/4 in.), but is designed to fit a majority of portable lamp applications. This version, with a core-coil ballast, results in a system efficacy of 54 lumens per watt, with a light output of more than 1800 lumens. This compares favorably with a 100-watt incandescent (17.5 lumens per watt and 1750 lumens light output). The color temperature of 3000/sup 0/K is compatible with an incandescent lamp (2800/sup 0/K). The color rendition index (CRI) is 84. With a solid-state ballast, the efficacy and light output could be increased by 20% (65 l/w, 2200 lumens) and could provide a direct replacement for a three-way, 150-watt incandescent lamp (15 l/w, 2200 lumens).

Morton, E.W.

1982-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Potential Environmental Impacts from the Metals in Incandescent, Compact Fluorescent Lamp (CFL), and Light-Emitting Diode (LED) Bulbs  

Science Journals Connector (OSTI)

Artificial lighting systems are transitioning from incandescent to compact fluorescent lamp (CFL) and light-emitting diode (LED) bulbs in response to the U.S. Energy Independence and Security Act and the EU Ecodesign Directive, which leads to energy savings and reduced greenhouse gas emissions. ... The CFLs and LEDs have higher resource depletion and toxicity potentials than the incandescent bulb due primarily to their high aluminum, copper, gold, lead, silver, and zinc. ... mercury pollution; redn. in electricity demand from the substitution of incandescent bulbs with fluorescents leads to reduced mercury emissions during the use of the bulb. ...

Seong-Rin Lim; Daniel Kang; Oladele A. Ogunseitan; Julie M. Schoenung

2012-12-13T23:59:59.000Z

22

A Review of the Reflector Compact Fluorescent Lights Technology Procurement Program: Conclusions and Results  

SciTech Connect (OSTI)

This report describes a project sponsored by the U.S. Department of Energy (DOE) and implemented by the Pacific Northwest National Laboratory (PNNL), from 2000 to 2007 to improve the performance of reflector type (R-lamp) compact fluorescent lamps (CFLs) and increase their availability throughout the United States by means of a technology development and procurement strategy. In 2000, at the request of the U.S. Department of Energys Emerging Technologies Program and its predecessors, the Pacific Northwest National Laboratory undertook a technology procurement seeking R-CFLs that were specifically designed for use in ICAT recessed can fixtures and that met other minimum performance criteria including minimum light output and size restrictions (to ensure they fit in standard residential recessed cans). The technology procurement included two phases. In Phase I, requests for proposals (RFPs) were issued in October 2002 and five manufacturers responded with 12 lamp models. Eight of these models met the minimum requirements and passed the 6-hour short-term test in a simulated ICAT environment. These eight models were subjected to long-term tests of 6,000 or more hours in a simulated ICAT environment. Three of these models passed the short- and long-term tests and were promoted through the program website (www.pnl.gov/rlamps), press releases, and fliers. To increase the number of qualifying models, a second RFP was issued in June 2005. In April 2007, DOE announced that 16 reflector CFL (R-CFL) models by four manufacturers had met all the minimum requirements of Phase 2 of the R-CFL Technology Innovation Competition. PNNL developed both the criteria and the test apparatus design for Elevated Temperature Life Testing (ETLT), which has been included by DOE in its draft ENERGY STAR specifications for the reflector category of CFLs. PNNL promoted the winning lamps through a program website, press releases, and fliers as well as through program partners. PNNL also helped engage distributors including Costco, the Home Depot, Bonneville Power Administration, and utility organizations.

Sandahl, Linda J.; Gilbride, Theresa L.; Ledbetter, Marc R.; McCullough, Jeffrey J.

2008-05-19T23:59:59.000Z

23

Synthesis Of Fluorescent Metal Nanoclusters  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Synthesis Of Fluorescent Metal Nanoclusters Synthesis Of Fluorescent Metal Nanoclusters Fluorescent metal nanoclusters were prepared. Available for thumbnail of Feynman Center...

24

EMSL - fluorescence spectrometer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

fluorescence-spectrometer en Structures and Stabilities of (MgO)n Nanoclusters. http:www.emsl.pnl.govemslwebpublicationsstructures-and-stabilities-mgon-nanoclusters

25

Single-molecule Fluorescence Spectroelectrochemistry of Cresyl...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

molecule Fluorescence Spectroelectrochemistry of Cresyl Violet. Single-molecule Fluorescence Spectroelectrochemistry of Cresyl Violet. Abstract: We coupled scanning fluorescence...

26

Fluorescent Tube Lamps  

Broader source: Energy.gov [DOE]

FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

27

Collecting Fluorescent Minerals  

Science Journals Connector (OSTI)

Good fluorescent mineral collections are the result of constant study and diligent search for superior material. As in the development of mineral collections generally, purchase and trade provide rewarding ave...

Manuel Robbins

1983-01-01T23:59:59.000Z

28

Fluorescent filtered electrophosphorescence  

DOE Patents [OSTI]

The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

Forrest, Stephen (Ann Arbor, MI); Sun, Yiru (Princeton, NJ); Giebink, Noel (Ann Arbor, MI); Thompson, Mark E. (Anaheim Hills, CA)

2010-08-03T23:59:59.000Z

29

Fluorescent filtered electrophosphorescence  

DOE Patents [OSTI]

The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

Forrest, Stephen R. (Princeton, NJ); Sun, Yiru (Princeton, NJ); Giebink, Noel (Princeton, NJ); Thompson, Mark E. (Anaheim Hills, CA)

2009-01-06T23:59:59.000Z

30

Renewable Surface Fluorescence Sandwich Immunoassay Biosensor...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Renewable Surface Fluorescence Sandwich Immunoassay Biosensor for Rapid Sensitive Botulinum Toxin Detection in an Automated Renewable Surface Fluorescence Sandwich Immunoassay...

31

Magnetic fluorescent lamp  

DOE Patents [OSTI]

The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

Berman, S.M.; Richardson R.W.

1983-12-29T23:59:59.000Z

32

Fluorescent microthermographic imaging  

SciTech Connect (OSTI)

In the early days of microelectronics, design rules and feature sizes were large enough that sub-micron spatial resolution was not needed. Infrared or IR thermal techniques were available that calculated the object`s temperature from infrared emission. There is a fundamental spatial resolution limitation dependent on the wavelengths of light being used in the image formation process. As the integrated circuit feature sizes began to shrink toward the one micron level, the limitations imposed on IR thermal systems became more pronounced. Something else was needed to overcome this limitation. Liquid crystals have been used with great success, but they lack the temperature measurement capabilities of other techniques. The fluorescent microthermographic imaging technique (FMI) was developed to meet this need. This technique offers better than 0.01{degrees}C temperature resolution and is diffraction limited to 0.3 {mu}m spatial resolution. While the temperature resolution is comparable to that available on IR systems, the spatial resolution is much better. The FMI technique provides better spatial resolution by using a temperature dependent fluorescent film that emits light at 612 nm instead of the 1.5 {mu}m to 12 {mu}m range used by IR techniques. This tutorial starts with a review of blackbody radiation physics, the process by which all heated objects emit radiation to their surroundings, in order to understand the sources of information that are available to characterize an object`s surface temperature. The processes used in infrared thermal imaging are then detailed to point out the limitations of the technique but also to contrast it with the FMI process. The FMI technique is then described in detail, starting with the fluorescent film physics and ending with a series of examples of past applications of FMI.

Barton, D.L.

1993-09-01T23:59:59.000Z

33

Fluorescent temperature sensor  

DOE Patents [OSTI]

The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

2009-03-03T23:59:59.000Z

34

Fluorescence analyzer for lignin  

DOE Patents [OSTI]

A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

Berthold, John W. (Salem, OH); Malito, Michael L. (Hubbard, OH); Jeffers, Larry (Alliance, OH)

1993-01-01T23:59:59.000Z

35

Development of a fluorescent cryocooler  

SciTech Connect (OSTI)

Recent work at Los Alamos National Laboratory has demonstrated the physical principles for a new type of solid-state cryocooler based on anti-Stokes fluorescence. Design studies indicate that a vibration-free, low-mass ``fluorescent cryocooler`` could operate for years with efficiencies and cooling powers comparable to current commercial systems. This paper presents concepts for a fluorescent cryocooler, design considerations and expected performance.

Edwards, B.C.; Buchwald, M.I.; Epstein, R.I.; Gosnell, T.R.; Mungan, C.E.

1995-10-01T23:59:59.000Z

36

Theory of delayed thermal fluorescence  

Science Journals Connector (OSTI)

A theory of nonradiative thermal activation involved in delayed thermal fluorescence has been developed from the viewpoint of the breakdown of the Born-Oppenheimer adiabatic approximation.

S. H. Lin

1971-01-01T23:59:59.000Z

37

X-ray fluorescence mapping  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

biololgical cells, over the measurement of impurities in solar cells, to the rare earth content of geological materials. A somewhat 'typical' layout for a X-ray fluorescence...

38

,,,"Incandescent","Standard Fluorescent","Compact Fluorescent...  

U.S. Energy Information Administration (EIA) Indexed Site

.",174,173,53,172,"Q","Q","Q" "Food Service ...",349,349,277,308,78,"Q",84 "Health Care ...",127,127,83,126,20,8,34 " Inpatient ...",11,...

39

SMB, X-ray Fluorescence Imaging  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fluorescence Imaging X-ray Fluorescence Imaging X-ray fluorescence imaging utilizes the high brightness of SPEAR3 and focused beam generated by the uses of K-B optics, capillaries...

40

Nuclear Resonance Fluorescence for Materials Assay  

E-Print Network [OSTI]

clandestine material with nuclear resonance fluorescence.E. Norman, UC Berkeley Dept. of Nuclear Engineering, privatepp. 349. G. Warren et al. Nuclear Resonance Fluorescence of

Quiter, Brian

2010-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Fluorescence, Super Resolution STORM Microscope | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

fluorescence imaging of the intact cell with nanometer resolution. It incorporates single-molecule fluorescence techniques to reconstruct super-resolution images using...

42

Single Nanoparticle Surface Enhanced Fluorescence  

Science Journals Connector (OSTI)

It is well established that when in close proximity to gold nanoparticles the optical properties of local fluorescent molecules are dramatically altered. When the localised surface plasmon resonance (LSPR), tuned...

Linden R. Webster; K. Suhling; D. Richards

2013-01-01T23:59:59.000Z

43

Nuclear Resonance Fluorescence for Nuclear Materials Assay  

E-Print Network [OSTI]

Potential of Nuclear Resonance Fluorescence . . . . . . . .2.9.1 Nuclear ThomsonSections . . . . . . . . . . . . . . . Nuclear Resonance

Quiter, Brian Joseph

2010-01-01T23:59:59.000Z

44

GREEN FLUORESCENT PROTEIN The green revolution  

E-Print Network [OSTI]

GREEN FLUORESCENT PROTEIN The green revolution Green fluorescent protein allows gene expression a fluorescent product when expressed. Just such a molecule, green fluorescent protein (GFP), has recently green light when disturbed (often seen when riding in a boat at night). In Aequorea, the green

Stearns, Tim

45

NEXT GENERATION ENERGY EFFICIENT FLUORESCENT LIGHTING PRODUCT  

SciTech Connect (OSTI)

This is the Final Report of the Next-Generation Energy Efficient Fluorescent Lighting Products program, Department of Energy (DOE). The overall goal of this three-year program was to develop novel phosphors to improve the color rendition and efficiency of compact and linear fluorescent lamps. The prime technical approach was the development of quantum-splitting phosphor (QSP) to further increase the efficiency of conventional linear fluorescent lamps and the development of new high color rendering phosphor blends for compact fluorescent lamps (CFLs) as potential replacements for the energy-hungry and short-lived incandescent lamps in market segments that demand high color rendering light sources. We determined early in the project that the previously developed oxide QSP, SrAl{sub 12}O{sub 19}:Pr{sup 3+}, did not exhibit an quantum efficiency higher than unity under excitation by 185 nm radiation, and we therefore worked to determine the physical reasons for this observation. From our investigations we concluded that the achievement of quantum efficiency exceeding unity in SrAl{sub 12}O{sub 19}:Pr{sup 3+} was not possible due to interaction of the Pr{sup 3+} 5d level with the conduction band of the solid. The interaction which gives rise to an additional nonradiative decay path for the excitation energy is responsible for the low quantum efficiency of the phosphor. Our work has led to the development of a novel spectroscopic method for determining photoionzation threshold of luminescent centers in solids. This has resulted in further quantification of the requirements for host phosphor lattice materials to optimize quantum efficiency. Because of the low quantum efficiency of the QSP, we were unable to demonstrate a linear fluorescent lamp with overall performance exceeding that of existing mercury-based fluorescent lamps. Our work on the high color rendering CFLs has been very successful. We have demonstrated CFLs that satisfies the EnergyStar requirement with color rendering index (CRI) greater than 90; the CRI of current commercial CFLs are in the low 80s. In this report we summarize the technical work completed under the Program, summarize our findings about the performance limits of the various technologies we investigated, and outline promising paths for future work.

Alok Srivastava; Anant Setlur

2003-04-01T23:59:59.000Z

46

Covered Product Category: Fluorescent Luminaires  

Broader source: Energy.gov [DOE]

FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

47

Covered Product Category: Fluorescent Ballasts  

Broader source: Energy.gov [DOE]

FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent ballasts, which are a FEMP-designated product category. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

48

Single molecule fluorescence (the basics)  

E-Print Network [OSTI]

Single molecule fluorescence (the basics) #12;light time light time No averaging, no need;knrkr S0 S1 Iph . . Iexc #12;Single molecule emitters #12;K. Brejc et.al., PNAS 94 (1997) 2306 1 nm glass, notch holographic, multidielectric,... #12;Optical schemes to detect single molecules Excitation

Ritort, Felix

49

Replacing Fluorescent Lightbulbs | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Replacing Fluorescent Lightbulbs Replacing Fluorescent Lightbulbs Replacing Fluorescent Lightbulbs July 29, 2012 - 6:37pm Addthis Although fluorescent lightbulbs are generally energy efficient, you can replace them with new, even more efficient bulbs that use better electrodes and coatings than older ones. | Photo courtesy of ©iStockphoto.com/JoLin. Although fluorescent lightbulbs are generally energy efficient, you can replace them with new, even more efficient bulbs that use better electrodes and coatings than older ones. | Photo courtesy of ©iStockphoto.com/JoLin. What does this mean for me? Today's fluorescent light fixtures and bulbs are far more efficient than older ones. You can replace fluorescent bulbs and ballasts with more efficient ones to save money and energy. All fluorescent bulbs contain a very small amount of mercury:

50

Statistics of Molecular Ensemble Blinking Fluorescence  

Science Journals Connector (OSTI)

Statistics of Molecular Ensemble Blinking Fluorescence ... Theoretical expressions suitable for statistical analysis of fluorescence of M molecules are derived. ... Therefore the probability of finding two photons at the same time equals zero. ...

I. S. Osadko; A. L. Shchukina

2010-05-19T23:59:59.000Z

51

Capacitive sensing with a fluorescent lamp  

E-Print Network [OSTI]

This work presents a modified fluorescent lamp that can be used as a capacitive sensing system. The lamp sensor measures changes in the electric fields emitted from the fluorescent bulbs in order to deduce the presence and ...

Cooley, John Jacob

2007-01-01T23:59:59.000Z

52

Clay Nanoparticle-Supported Single-Molecule FluorescenceSpectroelectr...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Clay Nanoparticle-Supported Single-Molecule Fluorescence Spectroelectrochemistry. Clay Nanoparticle-Supported Single-Molecule Fluorescence Spectroelectrochemistry. Abstract: We...

53

Fluorescent Lighting Basics | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Fluorescent Lighting Basics Fluorescent Lighting Basics Fluorescent Lighting Basics October 17, 2013 - 5:39pm Addthis Light from a fluorescent lamp is first created by an electric current conducted through an inert gas producing ultraviolet light that is invisible to the human eye. The ultraviolet light in turn interacts with special blends of phosphors coating the interior surface of the fluorescent lamp tube that efficiently converts the invisible light into useful white light. Fluorescent lamps require a special power supply called a ballast that is needed to regulate lamp operating current and provide a compatible start-up voltage. Electronic ballasts perform the same function as a magnetic ballast but outperform the outdated magnetic products by operating at a very high frequency that eliminates flicker and noise while

54

Chromosome characterization using single fluorescent dye  

DOE Patents [OSTI]

Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.

Crissman, Harry A. (Los Alamos, NM); Hirons, Gregory T. (Irvine, CA)

1995-01-01T23:59:59.000Z

55

Max Tech and Beyond: Fluorescent Lamps  

E-Print Network [OSTI]

varies by watts of power per foot of electrical arc in theFoot Linear Fluorescent Lamps Efficacy Life Lumens Type Base Power

Scholand, Michael

2012-01-01T23:59:59.000Z

56

Exciplex Fluorescence Thermometry of Liquid Fuel  

Science Journals Connector (OSTI)

An experimental program is described that investigates the application of exciplex fluorescence to the internal thermometry of flowing liquid decane in the temperature range 24-91C....

Stufflebeam, John H

1989-01-01T23:59:59.000Z

57

Energy saving controller for fluorescent lamps.  

E-Print Network [OSTI]

??Although fluorescent lamp is a very efficient lighting device in daily life, still the high harmonic distortion and low power factor cause unnecessary energy consumption. (more)

Cheong, Zhi Xiong

2010-01-01T23:59:59.000Z

58

LED Replacements for Linear Fluorescent Lamps Webcast  

Broader source: Energy.gov [DOE]

In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting...

59

Max Tech and Beyond: Fluorescent Lamps  

E-Print Network [OSTI]

Fluorescent Lamps and Incandescent Reflector Lamps; Finalmany end- use applications. Incandescent Outdoor Stationaryof color compared with the incandescent reference source is

Scholand, Michael

2012-01-01T23:59:59.000Z

60

Covered Product Category: Fluorescent Ballasts | Department of...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

can achieve large energy savings by moderating the timing and light output of lighting systems. Fluorescent lamp ballasts should have a ballast factor between 85% and 105% in...

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Fluorescence, Single-Molecule Microscope | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Single-Molecule Microscope Fluorescence, Single-Molecule Microscope The single-molecule optical microscope is designed to study complex reaction dynamics such as enzymatic...

62

Single-Molecule Fluorescence Analysis in Solution  

Science Journals Connector (OSTI)

Over the past five years, several groups have developed the capability to detect and identify single fluorescent molecules in solution as the molecules flow through a focused laser...

Keller, Richard A; Ambrose, W Patrick; Goodwin, Peter M; Jett, James H; Martin, John C; Wu, Ming

1996-01-01T23:59:59.000Z

63

Fluorescence lidar monitoring of historic buildings  

Science Journals Connector (OSTI)

Laser-induced fluorescence spectra detected with high-spectral-resolution lidar on the facades of the Baptistery and the Cathedral in Parma are presented and discussed. The...

Raimondi, Valentina; Cecchi, Giovanna; Pantani, Luca; Chiari, Roberto

1998-01-01T23:59:59.000Z

64

Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry  

E-Print Network [OSTI]

CHAPTER 17 Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry Kiryl D. PiatkevichH Stability of Fluorescence F. Optimization of Nucleotide and Amino Acid Sequences III. Modern Advanced Red-Shifted FPs A. Orange Fluorescent Proteins B. Red Fluorescent Proteins C. Far-Red Fluorescent Proteins IV

Verkhusha, Vladislav V.

65

PEVELOPMENT OF FLUORESCENCE LIFETIME DIAGNOSTIC  

Office of Scientific and Technical Information (OSTI)

4 4 PEVELOPMENT OF FLUORESCENCE LIFETIME DIAGNOSTIC w I Project Accomplishments Summary (Attachment I) CRADA NO. TSB-1449-97 Date: U 1 8 1 9 8 Revision: 1 A . Parties The project is a relationship between the Lawrence Livennore National Laboratoq (LLNL) and Optiphase, Inc. University of California Lawrence Livermore National Laboratory 7000 East Avenue, L-399 Livermore, CA 94550 Optiphase, h c 7652 Haskell Ave. Van Nuys, CA 91406 Technical Contact - D r . Pepe Davis (8 18)782-0997ext 1 12 B . Background Fiber-optic-based sensors are excellent candidates for detecting the presence and monitoring the levels of degradation products in stockpiled weapons. Specifically, fl uorescence-based sensors are extremely sensitive, can have high specificity for compounds of interest, and are "e~ectrically

66

Fiber optical assembly for fluorescence spectrometry  

DOE Patents [OSTI]

A system for analyzing a sample for the presence of an analyte in a sample. The system includes a sample holder for containing the sample; an excitation source, such as a laser, and at least one linear array radially disposed about the sample holder. Radiation from the excitation source is directed to the sample, and the radiation induces fluorescent light in the sample. Each linear array includes a plurality of fused silica optical fibers that receive the fluorescent light and transmits a fluorescent light signal from the first end to an optical end port of the linear array. An end port assembly having a photo-detector is optically coupled to the optical end port. The photo-detector detects the fluorescent light signal and converts the fluorescent light signal into an electrical signal.

Carpenter, II, Robert W. (Pagosa Springs, CO); Rubenstein, Richard (Staten Island, NY); Piltch, Martin (Los Alamos, NM); Gray, Perry (Los Alamos, NM)

2010-12-07T23:59:59.000Z

67

Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies  

Science Journals Connector (OSTI)

...Microbial Cell Biology Using Superfolder Green Fluorescent Protein for Periplasmic Protein...hampered by problems with the export of green fluorescent protein (GFP). Here we show...problems with the export of functional green fluorescent protein (GFP) (9). When...

Thuy Dinh; Thomas G. Bernhardt

2011-07-15T23:59:59.000Z

68

Near-Infrared Fluorescent 9-Phenylethynylpyronin Analogues for Bioimaging  

Science Journals Connector (OSTI)

The syntheses and biological applications of two novel fluorescent 9-phenylethynylpyronin analogues containing either carbon or silicon at the position 10 are reported. Both fluorescent probes exhibited a relatively strong fluorescence in methanol and ...

Tom Pastierik; Peter ebej; Ji?ina Medalov; Peter tacko; Petr Kln

2014-03-31T23:59:59.000Z

69

Building Technologies Office: Fluorescent and Incandescent Lamps Public  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Fluorescent and Fluorescent and Incandescent Lamps Public Meeting to someone by E-mail Share Building Technologies Office: Fluorescent and Incandescent Lamps Public Meeting on Facebook Tweet about Building Technologies Office: Fluorescent and Incandescent Lamps Public Meeting on Twitter Bookmark Building Technologies Office: Fluorescent and Incandescent Lamps Public Meeting on Google Bookmark Building Technologies Office: Fluorescent and Incandescent Lamps Public Meeting on Delicious Rank Building Technologies Office: Fluorescent and Incandescent Lamps Public Meeting on Digg Find More places to share Building Technologies Office: Fluorescent and Incandescent Lamps Public Meeting on AddThis.com... About Standards & Test Procedures Implementation, Certification & Enforcement

70

Fluorescent Silica Nanoparticles with Efficient Urinary Excretion for Nanomedicine  

Science Journals Connector (OSTI)

Fluorescent Silica Nanoparticles with Efficient Urinary Excretion for Nanomedicine ... The emerging nanomedicine landscape ... Core-shell silica nanoparticles as fluorescent labels for nanomedicine ...

Andrew A. Burns; Jelena Vider; Hooisweng Ow; Erik Herz; Oula Penate-Medina; Martin Baumgart; Steven M. Larson; Ulrich Wiesner; Michelle Bradbury

2008-12-19T23:59:59.000Z

71

Excitation-emission spectra and fluorescence quantum yields for...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Excitation-emission spectra and fluorescence quantum yields for fresh and aged biogenic secondary organic aerosols. Excitation-emission spectra and fluorescence quantum yields for...

72

2014-10-14 Issuance: Test Procedures Correction for Fluorescent...  

Broader source: Energy.gov (indexed) [DOE]

10-14 Issuance: Test Procedures Correction for Fluorescent Lamp Ballasts; Notice of Proposed Rulemaking 2014-10-14 Issuance: Test Procedures Correction for Fluorescent Lamp...

73

Single molecule fluorescence in rectangular nano-apertures  

Science Journals Connector (OSTI)

Fluorescence Correlation Spectroscopy is used to investigate fluorescent molecules in solution diffusing in subwavelength rectangular apertures milled in Aluminium films. This...

Wenger, Jrme; Lenne, Pierre-Franois; Popov, Evgueni; Rigneault, Herv; Dintinger, Jos; Ebbesen, Thomas

2005-01-01T23:59:59.000Z

74

Fluorescent Minerals of the United States  

Science Journals Connector (OSTI)

Possibly the most interesting and consistently fluorescing sphalerites are found at Franklin, New Jersey, and the Sterling mine at Ogdensburg, New Jersey. These sphalerites occur as resinous blebs or masses, c...

Manuel Robbins

1983-01-01T23:59:59.000Z

75

Epi-Fluorescence Inverted Microscope (Zeiss,  

E-Print Network [OSTI]

Epi-Fluorescence Inverted Microscope (Zeiss, Observer Z1) April 2013 #12;Start Up cont. · Turn) #12;Using the Microscope cont. · On an inverted microscope samples on a glass slide are usually viewed

Subramanian, Venkat

76

Intrinsic Fluorescence of Intrinsically Disordered Proteins  

Science Journals Connector (OSTI)

Resolution of the intrinsic emission properties of a protein by different fluorescence spectroscopy techniques is an ... on rotational movements of peptide chains or whole proteins. Here, we describe the details ...

Paolo Neyroz; Stefano Ciurli

2012-01-01T23:59:59.000Z

77

Multiplexed fluorescence detector system for capillary electrophoresis  

DOE Patents [OSTI]

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

Yeung, E.S.; Taylor, J.A.

1994-06-28T23:59:59.000Z

78

Multiplexed fluorescence detector system for capillary electrophoresis  

DOE Patents [OSTI]

A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

Yeung, E.S.; Taylor, J.A.

1996-03-12T23:59:59.000Z

79

Ultratrace analysis of transuranic actinides by laser-induced fluorescence  

DOE Patents [OSTI]

Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

Miller, S.M.

1983-10-31T23:59:59.000Z

80

Ultrabright fluorescent OLEDS using triplet sinks  

DOE Patents [OSTI]

A first device is provided. The first device further comprises an organic light emitting device. The organic light emitting device further comprises an anode, a cathode, and an emissive layer disposed between the anode and the cathode. The emissive layer further comprises an organic host compound, an organic emitting compound capable of fluorescent emission at room temperature, and an organic dopant compound. The triplet energy of the dopant compound is lower than the triplet energy of the host compound. The dopant compound does not strongly absorb the fluorescent emission of the emitting compound.

Zhang, Yifan; Forrest, Stephen R; Thompson, Mark

2013-06-04T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Covered Product Category: Suspended Fluorescent Luminaires  

Broader source: Energy.gov [DOE]

The Federal Energy Management Program (FEMP) provides acquisition guidance and Federal efficiency requirements for Suspended Fluorescent Luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

82

On simulating 3D fluorescent microscope images  

Science Journals Connector (OSTI)

In recent years many various biomedical image segmentation methods have appeared. Though typically presented to be successful the majority of them was not properly tested against ground truth images. The obvious way of testing the quality of new segmentation ... Keywords: convolution, fluorescent optical microscope, procedural texture, simulator, synthetic image

David Svoboda; Marek Kak; Martin Maka; Jan Hubeny; Stanislav Stejskal; Michal Zimmermann

2007-08-01T23:59:59.000Z

83

New and Underutilized Technology: Efficient High Bay Fluorescent Lighting |  

Broader source: Energy.gov (indexed) [DOE]

Efficient High Bay Fluorescent Efficient High Bay Fluorescent Lighting New and Underutilized Technology: Efficient High Bay Fluorescent Lighting October 7, 2013 - 8:54am Addthis The following information outlines key deployment considerations for efficient high bay fluorescent lighting within the Federal sector. Benefits Efficient high bay fluorescent lighting can include either T5 or T8 fluorescent lighting systems for high-bay applications currently using metal halide fixtures. Fluorescent fixtures offer better light distribution, better light maintenance over the life of the lamp, improved color quality, and on-off control (re-strike time) with lower energy consumption. Application Efficient high bay fluorescent lighting is applicable for facilities containing high bay areas. Key Factors for Deployment

84

CODED SPECTROSCOPY FOR ETHANOL DETECTION IN DIFFUSE, FLUORESCENT MEDIA  

E-Print Network [OSTI]

ABSTRACT CODED SPECTROSCOPY FOR ETHANOL DETECTION IN DIFFUSE, FLUORESCENT MEDIA by Scott Thomas Mc FOR ETHANOL DETECTION IN DIFFUSE, FLUORESCENT MEDIA by Scott Thomas McCain Department of Electrical

85

Fluorescence resonance energy transfer scanning near-field optical microscopy  

Science Journals Connector (OSTI)

...and applications. New York: Wiley Interscience...Fluorescence resonance energy transfer SNOM 919...fluorescence resonance energy transfer source...Electromagnetic theory. New York: McGraw-Hill...hierarchy and directed energy transfer in conjugated...

2004-01-01T23:59:59.000Z

86

Text-Alternative Version: LED Replacements for Linear Fluorescent Lamps  

Broader source: Energy.gov [DOE]

Below is the text-alternative version of the "LED Replacements for Linear Fluorescent Lamps" webcast, held June 20, 2011.

87

A photoactivatable green-fluorescent protein from the phylum Ctenophora  

Science Journals Connector (OSTI)

...Diversity and evolution of the green fluorescent protein family...determined by a nonfluorescent green fluorescent protein homolog...rhodamine 6G and fluorescein in nine solvents: improved absolute standards...structure of the Aequorea victoria green fluorescent protein. Gene 111...

2010-01-01T23:59:59.000Z

88

Red fluorescent proteins: chromophore formation and cellular applications  

E-Print Network [OSTI]

Red fluorescent proteins: chromophore formation and cellular applications Atsushi Miyawaki1,2 , Daria M Shcherbakova3,4 and Vladislav V Verkhusha3,4 In the last decade, a number of red fluorescent proteins (RFPs) that emit orange, red, and far-red fluorescence have been isolated from anthozoans (corals

Verkhusha, Vladislav V.

89

LIGHT-INDUCED CHANGES IN THE FLUORESCENCE YIELD OF  

E-Print Network [OSTI]

OF PREILLUMINATION ON THE FLUORESCENCE TRANSIENT OF Chlorella pyrenoidosa JOHN C. MUNDAY, JR., and GOVINDJEE From Mexico 88330. ABSTRACT The fluorescence transient of Chlorella pyrenoidosa, excited by saturat- ing blue Chlorella pyrenoidosa the fluorescence transient exhibits a base level 0, a hump I at 50 msec. (at 1.5 X 104

Govindjee

90

LIGHT-INDUCED CHANGES IN THE FLUORESCENCE YIELD OF  

E-Print Network [OSTI]

LIGHT-INDUCED CHANGES IN THE FLUORESCENCE YIELD OF CHLOROPHYLL a IN VIVO II. CHLORELLA PYRENOIDOSA in Chlorella pyrenoidosa consists of a fast rise of the fluorescence yield from the level S (of the first wave photophosphorylation. INTRODUCTION The long-term fluorescence induction in Chlorella pyrenoidosa (second wave

Govindjee

91

Brief Communication 809 Photoactivation turns green fluorescent protein red  

E-Print Network [OSTI]

Brief Communication 809 Photoactivation turns green fluorescent protein red Michael B. Elowitz gene was first cloned, the Aequorea victoria green fluorescent protein (GFP) has become a powerful tool- wavelength (red) fluorescence has remained elusive. Here we describe a red-emitting, green

Elowitz, Michael

92

Upping fluorescence Paving the way for single-molecule medical  

E-Print Network [OSTI]

Upping fluorescence ­ Paving the way for single-molecule medical sensors The presence to the information content of single molecule fluorescence measurement[2]. Ideally, a single fluorophore should last in the collection of data at the single-molecule level where oxygen efficiently reduces the fluorescence lifetime

Strathclyde, University of

93

Single-molecule Fluorescence Spectroelectrochemistry of Cresyl Violet  

SciTech Connect (OSTI)

We coupled scanning fluorescence microscopy with a potentiostat via a three-electrode electrochemical cell to enable single-molecule fluorescence spectroelectrochemistry of cresyl violet in aqueous solution, where the single-molecule fluorescence intensity of cresyl violet is modulated synchronously with the cyclic voltammetric potential scanning.

Lei, Chenghong; Hu, Dehong; Ackerman, Eric J.

2008-11-21T23:59:59.000Z

94

Limitations of the Reporter Green Fluorescent Protein under Simulated Tumor Conditions  

Science Journals Connector (OSTI)

...visualized live and in process by green fluorescence protein expression...Tsien R. Y. Engineering green fluorescent protein for improved...wavelengths and fluorescence resonance energy transfer. Curr. Biol., 6...Generation of destabilized green fluorescent protein as a transcription...

Claudia Coralli; Maja Cemazar; Chryso Kanthou; Gillian M. Tozer; and Gabi U. Dachs

2001-06-15T23:59:59.000Z

95

Product Standards for Fluorescent Lighting (Japan) | Open Energy  

Open Energy Info (EERE)

Product Standards for Fluorescent Lighting (Japan) Product Standards for Fluorescent Lighting (Japan) Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Product Standards for Fluorescent Lighting (Japan) Focus Area: Appliances & Equipment Topics: Policy Impacts Website: www.eccj.or.jp/top_runner/pdf/tr_fluorescent_lights_jul.2009.pdf Equivalent URI: cleanenergysolutions.org/content/product-standards-fluorescent-lightin Language: English Policies: "Deployment Programs,Regulations" is not in the list of possible values (Deployment Programs, Financial Incentives, Regulations) for this property. DeploymentPrograms: Industry Codes & Standards Regulations: Appliance & Equipment Standards and Required Labeling This Energy Conservation Center Japan (ECCJ) document was created as a

96

The Colorful Journey of Green Fluorescent Protein  

Science Journals Connector (OSTI)

Though the story of GFP began millions of years ago when the jellyfish Aequorea aequorea (also commonly referred to as Aequorea victoria and Aequorea forskalea) successfully evolved a fluorescent protein species with the uncanny ability to convert the excited blue energy of the bioluminescent protein aequorin into the green light observed in nature, its journey to Natures Scientific Contributors Hall of Fame started much more recently, in 1961. ... This observation, which represents the initial discovery of GFP, was included as a footnote in a paper by Shimomura and Johnson (1) describing the purification and characterization of aequorin: A protein giving solutions that look slightly greenish in sunlight though only yellowish under tungsten lights, and exhibiting a very bright, greenish fluorescence in the ultraviolet of a Mineralite, has also been isolated from the squeezates. ... In fact, during my time there, he still tried to find time to carry out chemical syntheses, which usually happened during Christmas. ...

Jin Zhang

2009-02-20T23:59:59.000Z

97

Measurement of optical efficiency of fluorescent luminaires  

SciTech Connect (OSTI)

By controlling the lamp wall temperature of fluorescent lamps with a Peltier device, the optical efficiency of luminaires can be measured directly. Measurements of the fixture efficiency for a luminaire can vary by over 8% depending upon the lamp-ballast system used in the measurement. The authors suggest that optical efficiency may be an improved metric for evaluating fixture designs. 6 refs., 3 figs., 5 tabs.

Verderber, R.R.; Morse, O.; Rubinstein, F.; Siminovitch, M.

1987-01-01T23:59:59.000Z

98

Invisible-fluorescent identification tags for materials  

DOE Patents [OSTI]

A taggant composition including a taggant material that is invisible in light of the visible spectrum and fluoresces under a non-visible excitation energy, a binder, and a solvent in which the taggant material and the binder are dissolved. The taggant composition can be printed or otherwise applied to a material such as fabric to provide a detectable and identifiable indicium. A method and apparatus for detecting and decoding the taggant indicium are also provided.

Lewis, Linda A.; Allgood, Glenn O.; Smithwick, III, Robert W.

2013-03-26T23:59:59.000Z

99

Covered Product Category: Compact Fluorescent Lamps | Department...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

per watt performance. Buyer Tips CFLs installed in enclosed fixtures designed for incandescent bulbs may overheat. This can significantly reduce both light output and lifetime....

100

Nuclear Resonance Fluorescence of 237 Np  

SciTech Connect (OSTI)

Identification and characterization of fissile material is of international concern because of the possibility of proliferation. For safeguards applications, techniques providing isotopic information are necessary [1]. However, few techniques can distinguish between fissile isotopes. Nuclear resonance fluorescence is one technique that can be used in applications to identify and quantify isotopic content. NRF has also been proposed in several detector systems as a viable way to identify fissile material in cargo containers [2, 3]. It is necessary to measure the characteristic resonances of each isotope that is to be distinguished in identification and assay applications.

Angell, Chris T.; Yee, R.; Joshi, T.; Swanberg, E.; Norman, E. B.; Hicks, C. L.; Klimenko, A.; Korbly, Steve; Wilson, C.; Kulp, W. D.; Warren, Glen A.; Bray, T. H.; Copping, R.; Glans, P.A.; Tyliszczak, T.; Shuh, David K.

2010-11-15T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Sensors and Actuators B 107 (2005) 121125 Two photon fluorescence sensors based on resonant grating  

E-Print Network [OSTI]

Sensors and Actuators B 107 (2005) 121­125 Two photon fluorescence sensors based on resonant the excitation and emission radia- tion reduces the background noise, the static photobleaching.07.040 #12;122 T. Katchalski et al. / Sensors and Actuators B 107 (2005) 121­125 lar, they are compact

Friesem, Asher A.

102

Solid state laser media driven by remote nuclear powered fluorescence  

DOE Patents [OSTI]

An apparatus is provided for driving a solid state laser by a nuclear powered fluorescence source which is located remote from the fluorescence source. A nuclear reaction produced in a reaction chamber generates fluorescence or photons. The photons are collected from the chamber into a waveguide, such as a fiber optic waveguide. The waveguide transports the photons to the remote laser for exciting the laser.

Prelas, Mark A. (Columbia, MO)

1992-01-01T23:59:59.000Z

103

Energy Dependence of Air Fluorescence Yield measured by AIRFLY  

E-Print Network [OSTI]

In the fluorescence detection of ultra high energy (> 10**18 eV) cosmic rays, the number of emitted fluorescence photons is assumed to be proportional to the energy deposited in air by shower particles. We have performed measurements of the fluorescence yield in atmospheric gases excited by electrons over energies ranging from keV to hundreds of MeV in several accelerators. We found that within the measured energy ranges the proportionality holds at the level of few %.

M. Ave

2007-11-28T23:59:59.000Z

104

Using fluorescence lifetime for discriminating detector afterpulsing in fluorescence-correlation spectroscopy  

E-Print Network [OSTI]

ACF , i.e., the second- order correlation function of the fluorescence intensity sig- nal. The physical meaning of the ACF is that it is propor- tional to the probability to detect, on average, a photon- nique, although the analysis does not explicitly identify single molecule detection events. On different

Enderlein, Jörg

105

Fluorescence methods for determination of temperature in fuel sprays  

Science Journals Connector (OSTI)

Fluorescent additives which allow the determination of droplet temperatures in hydrocarbon fuel sprays have been developed. These systems, which exploit the chemistry of organic...

Murray, A M; Melton, L A

1985-01-01T23:59:59.000Z

106

Site-Specific Synthesis and In Situ Immobilization of Fluorescent...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Site-Specific Synthesis and In Situ Immobilization of Fluorescent Silver Nanoclusters on DNA Nanoscaffolds by Use of the Tollens Reaction Authors: Pal, S., Varghese, R., Deng, Z.,...

107

Time-resolved fluorescence decay measurements for flowing particles  

DOE Patents [OSTI]

Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.

Deka, Chiranjit (Miami, FL); Steinkamp, John A. (Los Alamos, NM)

1999-01-01T23:59:59.000Z

108

Time-resolved fluorescence decay measurements for flowing particles  

DOE Patents [OSTI]

Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.

Deka, C.; Steinkamp, J.A.

1999-06-01T23:59:59.000Z

109

Source Apportionment of Wastewater Using Bayesian Analysis of Fluorescence Spectroscopy.  

E-Print Network [OSTI]

??This research uses Bayesian analysis of fluorescence spectroscopy results to determine if wastewater from the Heber Valley Special Service District (HVSSD) lagoons in Midway, UT (more)

Blake, Daniel B.

2014-01-01T23:59:59.000Z

110

Fluorescent Pigments for High-Performance Cool Roofing and Facades...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

3 Berkeley Lab Heat Island Group physicist Paul Berdahl inserts precursor solution into a furnace. After boiling and then combustion, a fluorescent red pigment is formed. Image:...

111

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution  

Science Journals Connector (OSTI)

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution ... The green-fluorescent protein (GFP) that functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea was renatured with ?90% yield following acid, base, or guanidine denaturation. ... Detailed output, in the form of structure plots with or without reliability information, single strand frequency plots and energy dot plots', are available for the folding of single sequences. ...

Grigory S. Filonov; Jared D. Moon; Nina Svensen; Samie R. Jaffrey

2014-10-22T23:59:59.000Z

112

Low Temperature Fluorescence Studies of Crude Petroleum Oils  

Science Journals Connector (OSTI)

(26) However, it has to be noted that dilution of crude petroleum oils radically changes the photophysics of fluorescence emission and, as such, the Shpolskii method is not suitable for exploring crude oil fluorescence from neat, undiluted oils. ... with shorter wavelength excitation (to 325 nm); all crude petroleums have nearly the same relative dependence of quantum yield on excitation wavelength. ...

Peter Owens; Alan G. Ryder

2011-09-14T23:59:59.000Z

113

A monomeric red fluorescent protein Robert E. Campbell*, Oded Tour*  

E-Print Network [OSTI]

A monomeric red fluorescent protein Robert E. Campbell*, Oded Tour* , Amy E. Palmer*, Paul A, and the obligate tetramerization of the red fluorescent protein from Discosoma (DsRed). Although the weak, the obligate tet- ramerization of DsRed has greatly hindered its use as a genetically encoded fusion tag. We

Tsien, Roger Y.

114

Fluorescent Sensors for Zn2+ Based on a Fluorescein Platform  

E-Print Network [OSTI]

Fluorescent Sensors for Zn2+ Based on a Fluorescein Platform: Synthesis, Properties fluorescent sensors for Zn2+ that utilize fluorescein as a reporting group, Zinpyr-1 and Zinpyr-2, have been. Both Zinpyr sensors have excitation and emission wavelengths in the visible range (500 nm

Tsien, Roger Y.

115

Green Fluorescent Protein Is Lighting Up Fungal Biology  

Science Journals Connector (OSTI)

...MINIREVIEW Green Fluorescent Protein Is Lighting Up Fungal Biology J. M. Lorang R...naturally occur, in a lab or a contained greenhouse, will present other challenges. However...host. . Green fluorescent protein is lighting up fungal biology. | Department of Botany...

J. M. Lorang; R. P. Tuori; J. P. Martinez; T. L. Sawyer; R. S. Redman; J. A. Rollins; T. J. Wolpert; K. B. Johnson; R. J. Rodriguez; M. B. Dickman; L. M. Ciuffetti

2001-05-01T23:59:59.000Z

116

Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent  

E-Print Network [OSTI]

Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent proteins Arie Krumholz1 the application of two spectrally distinct near-infrared fluorescent proteins, iRFP670 and iRFP720, engineered-tissue PAT, probes absorbing in the near-infrared (NIR) spectral range are desirable. In the NIR optical

Verkhusha, Vladislav V.

117

Full-wavelet approach for fluorescence diffuse optical tomography  

E-Print Network [OSTI]

Full-wavelet approach for fluorescence diffuse optical tomography with structured illumination Leonardo da Vinci 32, I-20133 Milan, Italy 3 Centre for Medical Image Computing, University College London); published October 28, 2010 We present a fast reconstruction method for fluorescence optical tomography

Boyer, Edmond

118

Abstract 4138: Fluorescence laparoscopy with an LED light source enables fluorescence-guided resection of pancreatic cancer, labeled with fluorophore-conjugated antibodies, in mouse models.  

Science Journals Connector (OSTI)

...4138: Fluorescence laparoscopy with an LED light source enables fluorescence-guided...495-nm emission filter and a Stryker L9000 LED light source 24 hours after tail vein injection...Bouvet. Fluorescence laparoscopy with an LED light source enables fluorescence-guided...

Cristina A. Metildi; Sharmeela Kaushal; George A. Luiken; Mark A. Talamini; Robert M. Hoffman; Michael Bouvet

2013-04-15T23:59:59.000Z

119

Nuclear Resonance Fluorescence for Materials Assay  

SciTech Connect (OSTI)

This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has ben performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX?s photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

Quiter, Brian J.; Ludewigt, Bernhard; Mozin, Vladimir; Prussin, Stanley

2009-06-29T23:59:59.000Z

120

Nuclear Resonance Fluorescence for Materials Assay  

SciTech Connect (OSTI)

This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has ben performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX's photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

Quiter, Brian; Ludewigt, Bernhard; Mozin, Vladimir; Prussin, Stanley

2009-06-05T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

X-Ray Fluorescence (XRF) | Open Energy Information  

Open Energy Info (EERE)

X-Ray Fluorescence (XRF) X-Ray Fluorescence (XRF) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Technique: X-Ray Fluorescence (XRF) Details Activities (0) Areas (0) Regions (0) NEPA(0) Exploration Technique Information Exploration Group: Lab Analysis Techniques Exploration Sub Group: Rock Lab Analysis Parent Exploration Technique: Rock Lab Analysis Information Provided by Technique Lithology: Bulk and trace element analysis of rocks, minerals, and sediments. Stratigraphic/Structural: Hydrological: Thermal: Dictionary.png X-Ray Fluorescence (XRF): X-Ray Fluorescence is a lab-based technique used for bulk chemical analysis of rock, mineral, sediment, and fluid samples. The technique depends on the fundamental principles of x-ray interactions with solid materials, similar

122

X-RAY FLUORESCENCE MICROPROBE (XFM) TECHNIQUES AND CAPABILITIES  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

RAY FLUORESCENCE MICROPROBE (XFM) RAY FLUORESCENCE MICROPROBE (XFM) TECHNIQUES AND CAPABILITIES APPLICATIONS WORLD-LEADING MICROFOCUSED EXAFS SPECTROSCOPY * XFM is an optimized three-pole wiggler beamline for the characterization of materials in an "as-is" state that are chemically heterogeneous at the micrometer scale via synchrotron induced X-ray fluorescence. * XFM includes instrumentation for microbeam X-ray fluorescence (µXRF), diffraction (µXRD) and fluorescence computed microtomography (FCMT) . However, it is optimized to provide users state-of-the-art microfocused Extended X-ray Absorption Fine Structure (µEXAFS) spectroscopy between 4 to 20 keV. * XFM will trade-off beam size and flux for sample configuration flexibility. This includes more readily achievable stability

123

L-Shell Fluorescence Yields of Pt, Tl, and Pb  

Science Journals Connector (OSTI)

Partial L-shell fluorescence yields for three heavy elements have been measured using an x-ray coincidence counting method. Vacancies in the K shell of the atom are produced either by K-electron capture or internal conversion of a nuclear gamma ray in the K shell. The coincidence rate between the K and L x rays observed after the creation of the K vacancy determines the partial fluorescence yield, ?KL. This quantity is defined as the fluorescence yield of those vacancies in the L shell created by K?1 and K?2 x-ray emission. In some cases, it was also possible to determine the partial fluorescence yield, ?LL of the L shell following L-electron capture. The results obtained are in reasonable agreement with previous measurements. The relationship between ?KL, ?LL, and the fluorescence yields of individual L subshells is discussed.

R. C. Jopson; Hans Mark; C. D. Swift

1962-12-15T23:59:59.000Z

124

Fluorescence cross section measurements of biological agent simulants  

SciTech Connect (OSTI)

Fluorescence is a powerful technique that has potential uses in detection and characterization of biological aerosols both in the battlefield and in civilian environments. Fluorescence techniques can be used with ultraviolet (UV) light detection and ranging (LIDAR) equipment to detect biological aerosol clouds at a distance, to provide early warning of a biological attack, and to track an potentially noxious cloud. Fluorescence can also be used for detection in a point sensor to monitor biological materials and to distinguish agents from benign aerosols. This work is part of a continuing program by the Army`s Chemical and Biological Defense Command to characterized the optical properties of biological agents. Reported here are ultraviolet fluorescence measurements of Bacillus megaterium and Bacillus Globigii aerosols suspended in an electrodynamic particle trap. Fluorescence spectra of a common atmospheric aerosol, pine pollen, are also presented.

Stephens, J.R.

1996-11-01T23:59:59.000Z

125

Green Fluorescent Protein Reporter System with Transcriptional Sequence Heterogeneity for Monitoring the Interferon Response  

Science Journals Connector (OSTI)

...immediate free access to this article. Green Fluorescent Protein Reporter System with...validated a live cell-based enhanced green fluorescent protein (EGFP) reporter system...validated a live cell-based enhanced green fluorescent protein (EGFP) reporter system...

Linah Mahmoud; Maher Al-Saif; Haitham M. Amer; Mustafa Sheikh; Fahad N. Almajhdi; Khalid S. A. Khabar

2011-07-13T23:59:59.000Z

126

X-ray fluorescence observations of the moon by SMART-1/D-CIXS and the first detection of Ti Ka from the lunar surface  

E-Print Network [OSTI]

X-ray fluorescence observations of the moon by SMART-1/D-CIXS and the first detection of Ti Ka from s t r a c t The demonstration of a compact imaging X-ray spectrometer (D-CIXS), which flew on ESA new technologies for orbital X-ray fluorescence spectroscopy. D-CIXS conducted observations

Wieczorek, Mark

127

A Red Cy3-Based Biarsenical Fluorescent Probe Targeted to a Complement...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Red Cy3-Based Biarsenical Fluorescent Probe Targeted to a Complementary Binding Peptide. A Red Cy3-Based Biarsenical Fluorescent Probe Targeted to a Complementary Binding Peptide....

128

Sustainable LED Fluorescent Light Replacement Technology  

SciTech Connect (OSTI)

Ilumisys and the National Center for Manufacturing Sciences (NCMS) partnered on a three-year project awarded by the United States (U.S.) Department of Energy (DOE), to quantify the impacts of LED lamps, incandescent lamps and fluorescent benchmark lamps over a product lifecycle i.e. to develop a sustainable design and manufacturing strategy that addresses product manufacturing, use, recycling and disposal scenarios for LED-based lighting. Based on the knowledge gained from extensive product tear-down studies of fluorescent and screw-in lighting products, lifecycle assessment tools, and accelerated lifecycle testing protocols, an interactive Sustainable LED Design Guide has been developed to aid architectural and lighting designers and engineers in making design decisions that consider three important environmental impacts (greenhouse gas emissions, energy use and mercury emission) across all phases of the life of an LED lighting product. Critical information developed for the lifecycle analysis and product feature comparisons is the useful life of the lighting product as well as its performance. The Design Guide is available at www.ncms.org, and was developed based on operational and durability testing of a variety of lighting products including power consumption, light output, and useful life of a lamp in order to allow a more realistic comparison of lamp designs. This report describes the main project tasks, results and innovative features of the lifecycle assessment (LCA)-based design tools, and the key considerations driving the sustainable design of LED lighting systems. The Design Guide incorporates the following three novel features for efficiently evaluating LED lighting features in value-chains: Bill-of-Materials (BOM) Builder Designers may import process data for each component and supply functional data for the product, including power, consumption, lumen output and expected useful life. Environmental Impact Review Designs are comparable across lifecycle phases, subsystems, and environmental impact category, and can be normalized to a userdefined functional unit. Drill-down Review These provide an indepth look at individual lamp designs with the ability to review across subsystem or lifecycle phase.

None

2011-06-30T23:59:59.000Z

129

Covered Product Category: Fluorescent Luminaires | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Fluorescent Luminaires Fluorescent Luminaires Covered Product Category: Fluorescent Luminaires October 7, 2013 - 10:52am Addthis Did You Know? Lighting Can Help You Accomplish Other Facility Upgrades and Efficiency Improvements Facilities with significant potential savings in lighting may be able to bundle lighting upgrades with heating, ventilation, air conditioning, and other energy conservation measures into a Utility Energy Service Contract (UESC) or Energy Savings Performance Contract (ESPC). Because of the substantial cost and energy savings potential, replacing lighting can be a key component in financing facility retrofits. The funding portion of the FEMP website offers more information on these and other funding opportunities. FEMP provides acquisition guidance and Federal efficiency requirements

130

Clay Nanoparticle-Supported Single-Molecule Fluorescence Spectroelectrochemistry  

SciTech Connect (OSTI)

We report single-molecule fluorescence spectroelectrochemistry on a clay-modified ITO electrode using cresyl violet as a redox fluorescent probe. Ensemble averaged experiments show that cresyl violet displays well-defined cyclic voltammograms when adsorbed on the clay-modified electrode. By probing the fluorescence intensity of a single cresyl violet molecule absorbed on clay surface, we can trace the redox reaction of individual molecules induced by the cyclic voltammetric potential scanning. Inhomogeneous interfacial electron transfer dynamics of the immobilized single cresyl violet molecules on the clay-modified surface were observed.

Lei, Chenghong; Hu, Dehong; Ackerman, Eric J.

2009-02-11T23:59:59.000Z

131

Federal Energy Management Program: Energy Cost Calculator for Compact  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Compact Fluorescent Lamps to someone by E-mail Compact Fluorescent Lamps to someone by E-mail Share Federal Energy Management Program: Energy Cost Calculator for Compact Fluorescent Lamps on Facebook Tweet about Federal Energy Management Program: Energy Cost Calculator for Compact Fluorescent Lamps on Twitter Bookmark Federal Energy Management Program: Energy Cost Calculator for Compact Fluorescent Lamps on Google Bookmark Federal Energy Management Program: Energy Cost Calculator for Compact Fluorescent Lamps on Delicious Rank Federal Energy Management Program: Energy Cost Calculator for Compact Fluorescent Lamps on Digg Find More places to share Federal Energy Management Program: Energy Cost Calculator for Compact Fluorescent Lamps on AddThis.com... Energy-Efficient Products Federal Requirements Covered Product Categories

132

Information Resources: LED Replacements for Linear Fluorescent Lamps  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Replacements for Linear Fluorescent Lamps Webcast Replacements for Linear Fluorescent Lamps Webcast In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting Facts-listed products as well as products evaluated in the latest CALiPER reports. Eric Richman, also of PNNL, reported on a recently completed GATEWAY demonstration project, in which LED and fluorescent lamps were installed in a variety of recessed troffer luminaires for comparison in an office environment. The presentation concluded with a discussion of specifications listed in a newly updated technology fact sheet. View presentation slides View the text-alternative version View the webcast (WMV 16 MB) Download Windows Media Player

133

" A Heterodyne Laser-induced Fluorescence Technique to Determine  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A Heterodyne Laser-induced Fluorescence Technique to Determine A Heterodyne Laser-induced Fluorescence Technique to Determine Simultaneously the Bulk and Time Varying Molecule Velocity Distribution." Inventors Ahmed Diallo, Stephane Mazouffre.The method's primary goal is to determine simultaneously the bulk a The method's primary goal is to determine simultaneously the bulk and the time verying part of the molecule velocity distribution using a heterodyne laser induced fluorescence technique. Used in biology, chemistry and plasma physics laser-induced fluorescence is a well known technique to resolve the bulk velocity distribution of probed molecules and atoms in a medium. The novel approach is aimed at determining not only the bulk distribution, but also the time-varying velocity distribution. The two parts of the velocity distribution are key in the characterization of a

134

A continuous reading fluorescence meter for eddy diffusion studies  

E-Print Network [OSTI]

LIBRARY A AIS COLLEGE OF TEXAS A CONTINUOUS READINO FLUORESCENCE METER FOR EDDY DIFFUSION STUDIES BY Frank Wooten Moon, Jr. A Thesis Submitted to ths Qraduate Scbool of the Agricultural and Mechanical College oi' Texas in partial...

Moon, Frank Wooten

1956-01-01T23:59:59.000Z

135

LIGHT-INDUCED CHANGES IN THE FLUORESCENCE YIELD OF  

E-Print Network [OSTI]

a in system II, the system which sensitizes the oxygen-evolving reaction (7-14). The factor controlling fluorescence intensity is the redox state of Q, the electron acceptor in the system It reaction center (9). Fig

Govindjee

136

Calibration of fluorescence resonance energy transfer in microscopy  

DOE Patents [OSTI]

Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

Youvan, Douglas C. (San Jose, CA); Silva, Christopher M. (Sunnyvale, CA); Bylina, Edward J. (San Jose, CA); Coleman, William J. (Moutain View, CA); Dilworth, Michael R. (Santa Cruz, CA); Yang, Mary M. (San Jose, CA)

2002-09-24T23:59:59.000Z

137

Engineering cell-fluorescent ion track hybrid detectors  

Science Journals Connector (OSTI)

The lack of sensitive biocompatible particle track detectors has so far limited parallel detection of physical energy deposition and biological response. Fluorescent nuclear track detectors (FNTDs) based on Al2O3

Martin Niklas; Steffen Greilich; Claudius Melzig; Mark S Akselrod

2013-06-01T23:59:59.000Z

138

Rapid identification of bacteria using an umbelliferone fluorescent assay  

E-Print Network [OSTI]

RAPID IDENTIFICATION OF BACTERIA USING AN UMBELLIFERONE FLUORESCENT ASSAY A Thesis by RICHARD THOMAS CHAMBLIN, JR, Submitted to the Graduate College of' Texas A&M University in partial fulfillment of the requirement for the degree of MASTER... OF SCIENCE August 1983 Major Subject: Veterinary Microbiology RAPID IDENTIFICATION OF BACTERIA USING AN UMBELLIFERONE FLUORESCENT ASSAY A Thesis by RICHARD THOMAS CHAMBLIN, JR ~ Approved as to style and content by ( -Chairman of Committee) John M...

Chamblin, Richard Thomas

1983-01-01T23:59:59.000Z

139

A light diet for a giant appetite: An assessment of China's proposed fluorescent lamp standard  

E-Print Network [OSTI]

standard for linear fluorescent lamps Length (mm) Minimum efficacy (lm/W) Efficacy for energy conservation

Lin, Jiang

2002-01-01T23:59:59.000Z

140

Fluorescent analysis of interaction of flavonols with hemoglobin and bovine serum albumin  

Science Journals Connector (OSTI)

We have studied the fluorescent properties of flavonols (quercetin, fisetin, morin, rutin) with the aim of...

V. V. Sentchouk; E. V. Bondaryuk

2007-09-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Development of rapid microbial methods for lysine quantification in feed ingredients based on green fluorescent protein fluorescence  

E-Print Network [OSTI]

fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Chair of Committee, Steven C. Ricke Committee Members, Deborah Siegele Jean Gould Dorothy Shippen Chair of Molecular... on Green Fluorescent Protein Fluorescence. (December 2005) Vesela I. Chalova-Zhekova, Diplom., Higher Institute of Food and Flavor Industries Chair of Advisory Committee: Dr. Steven Ricke Lysine is one of the more limiting amino acids in protein...

Chalova-Zhekova, Vesela I.

2007-04-25T23:59:59.000Z

142

A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent  

Broader source: Energy.gov (indexed) [DOE]

A Bright Idea: New Efficiency Standards for Incandescent and A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent Lights A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent Lights July 21, 2009 - 5:18pm Addthis John Lippert Pretty soon, lighting is going to get a lot more efficient. New standards for incandescent reflector bulbs, general purpose fluorescent bulbs, and regular incandescent bulbs are going into effect beginning in approximately three years. You may be curious about how these standards will affect the most popular types of incandescent bulbs we've all used for so long: the common non-reflector 40-watt, 60-watt, 75-watt, and 100-watt bulbs. The Energy Independence and Security Act of 2007 (also known as EISA) requires that these incandescent bulbs use 30% less energy than today's

143

Laser-induced differential normalized fluorescence method for cancer diagnosis  

DOE Patents [OSTI]

An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample. 5 figs.

Vo-Dinh, T.; Panjehpour, M.; Overholt, B.F.

1996-12-03T23:59:59.000Z

144

Laser-induced differential normalized fluorescence method for cancer diagnosis  

DOE Patents [OSTI]

An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.

Vo-Dinh, Tuan (Knoxville, TN); Panjehpour, Masoud (Knoxville, TN); Overholt, Bergein F. (Knoxville, TN)

1996-01-01T23:59:59.000Z

145

BIOCOMPATIBLE FLUORESCENT MICROSPHERES: SAFE PARTICLES FOR MATERIAL PENETRATION STUDIES  

SciTech Connect (OSTI)

Biocompatible polymers with hydrolyzable chemical bonds have been used to produce safe, non-toxic fluorescent microspheres for material penetration studies. The selection of polymeric materials depends on both biocompatibility and processability, with tailored fluorescent properties depending on specific applications. Microspheres are composed of USFDA-approved biodegradable polymers and non-toxic fluorophores and are therefore suitable for tests where human exposure is possible. Micropheres were produced which contain unique fluorophores to enable discrimination from background aerosol particles. Characteristics that affect dispersion and adhesion can be modified depending on use. Several different microsphere preparation methods are possible, including the use of a vibrating orifice aerosol generator (VOAG), a Sono-Tek atomizer, an emulsion technique, and inkjet printhead. Applications for the fluorescent microspheres include challenges for biodefense system testing, calibrants for biofluorescence sensors, and particles for air dispersion model validation studies.

Farquar, G; Leif, R

2009-07-15T23:59:59.000Z

146

Fluorescent spectra of chromatographic fractions of crude oils  

E-Print Network [OSTI]

Number 4 exhibits the j400 Ao peak. Again all Fig. 7 0 Fluorescent Spectra of n-heptane Fraction, Cut No. 2 ()) 0 fE 30 ( 3) / (lI I I I I (&) )/ I 000 travelength in Ao significant intensity ranges from 3400 A to 4600 A... as $ Transnlssion Cyclohexane Fraction 7'ave Length& Ao Relative Fluorescent Intensity ude o 3000 3100 3200 3300 3400 3500 3600 3F' jwo 4100 4200 4300 4400 4500 4600 QOO 4900 5000 5200 5400 56OO 5'800 6000 65oo 7000 Oe0 Oeo O. e 2 ' 4...

Dixon, William Samuel

2012-06-07T23:59:59.000Z

147

A versatile detector for total fluorescence and electron yield experiments  

SciTech Connect (OSTI)

The combination of a non-coated silicon photodiode with electron repelling meshes makes a versatile detector for total fluorescence yield and electron yield techniques highly suitable for x-ray absorption spectroscopy. In particular, a copper mesh with a bias voltage allows to suppress or transmit the electron yield signal. The performance of this detection scheme has been characterized by near edge x-ray absorption fine structure studies of thermal oxidized silicon and sapphire. The results show that the new detector probes both electron yield and for a bias voltage exceeding the maximum photon energy the total fluorescence yield.

Thielemann, N. [Institute for Methods and Instrumentation for Synchrotron Radiation Research, Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Institut fuer Physik, Humboldt-Universitaet zu Berlin, Newtonstrasse 15, 12489 Berlin (Germany); Hoffmann, P. [Institute for Methods and Instrumentation for Synchrotron Radiation Research, Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Foehlisch, A. [Institute for Methods and Instrumentation for Synchrotron Radiation Research, Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin (Germany); Institut fuer Physik und Astronomie, Universitaet Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam (Germany)

2012-09-15T23:59:59.000Z

148

BIOCOMPATIBLE FLUORESCENT MICROSPHERES: SAFE PARTICLES FOR MATERIAL PENETRATION STUDIES  

SciTech Connect (OSTI)

Biocompatible polymers with hydrolyzable chemical bonds are being used to produce safe, non-toxic fluorescent microspheres for material penetration studies. The selection of polymeric materials depends on both biocompatibility and processability, with tailored fluorescent properties depending on specific applications. Microspheres are composed of USFDA-approved biodegradable polymers and non-toxic fluorophores and are therefore suitable for tests where human exposure is possible. Micropheres are being produced which contain unique fluorophores to enable discrimination from background aerosol particles. Characteristics that affect dispersion and adhesion can be modified depending on use. Several different microsphere preparation methods are possible, including the use of a vibrating orifice aerosol generator (VOAG), a Sono-Tek atomizer, an emulsion technique, and inkjet printhead. The advantages and disadvantages of each method will be presented and discussed in greater detail along with fluorescent and charge properties of the aerosols. Applications for the fluorescent microspheres include challenges for biodefense system testing, calibrants for biofluorescence sensors, and particles for air dispersion model validation studies.

farquar, G; Leif, R

2008-09-12T23:59:59.000Z

149

Supporting Information for: Synthetic Control of Green Fluorescent Protein (GFP)  

E-Print Network [OSTI]

, and then using DNA ligase to insert synthetic oligonucleotides containing the loop sequence were inserted usingS1 Supporting Information for: Synthetic Control of Green Fluorescent Protein (GFP) Kevin P. Kent the genes in pET-15b with restriction enzymes that cut the DNA close to the desired loop insertion site

Boxer, Steven G.

150

A genetically encoded fluorescent reporter of ATP:ADP ratio  

E-Print Network [OSTI]

A genetically encoded fluorescent reporter of ATP:ADP ratio Jim Berg1,2, Yin Pun Hung1 & Gary's affinity for Mg-ATP was o100 nM, as seen for other members of the bacterial PII regulator family, a surprisingly high affinity given that normal intracellular ATP concentration is in the millimolar range. ADP

Yellen, Gary

151

Stepwise Quenching of Exciton Fluorescence in Carbon Nanotubes  

E-Print Network [OSTI]

1 Stepwise Quenching of Exciton Fluorescence in Carbon Nanotubes by Single Molecule Reactions with individual single-walled carbon nanotubes are observed, and luminescence quenching analysis reveals the diffusional range of excitons in semiconducting nanotubes. *To whom correspondence should be addressed

Paris-Sud XI, Université de

152

Spotlights on Recent JACS Publications FLUORESCENT PROBES LIGHT UP AMYLOID  

E-Print Network [OSTI]

the conductance, or transport, of ions across the membrane. Researchers have exploited the efficient transport investigated the mechanism of ion transport through several structural variants of fluorescent oligoester: the channel's ion transport activity slowly decreased over time. The researchers used steady-state and time

Theodorakis, Emmanuel

153

Cellubrevin-targeted Fluorescence Uncovers Heterogeneity in the Recycling Endosomes*  

E-Print Network [OSTI]

Cellubrevin-targeted Fluorescence Uncovers Heterogeneity in the Recycling Endosomes* (Received, University of California, Berkeley, California 94720-3200 The pH and trafficking of recycling endosomes have-enriched recycling endosomes (pHCb) and FITC-transferrin to measure the pH of transferrin- enriched recycling

Machen, Terry E.

154

REVIEW Open Access Environment-sensitive behavior of fluorescent  

E-Print Network [OSTI]

REVIEW Open Access Environment-sensitive behavior of fluorescent molecular rotors Mark A Haidekker1 and the molecular-scale interaction of a molecular rotor with its environment are not fully understood. This review rotors as microviscosity sensors are reviewed, and engineering aspects are presented on how measurement

Theodorakis, Emmanuel

155

Optical gating of perylene bisimide fluorescence using dithienylcyclopentene photochromic switches  

SciTech Connect (OSTI)

The emission of millions of fluorescence photons from a chromophore is controlled by the absorption of a few tens of photons in a photochromic molecule. The parameters that determine the efficiency of this process are investigated, providing insights for the development of an all-optical gate.

Prs, Martti; Khler, Jrgen, E-mail: juergen.koehler@uni-bayreuth.de [Experimental Physics IV, University of Bayreuth, 95440 Bayreuth (Germany)] [Experimental Physics IV, University of Bayreuth, 95440 Bayreuth (Germany); Grf, Katja; Bauer, Peter; Thelakkat, Mukundan [Applied Functional Polymers, University of Bayreuth, 95440 Bayreuth (Germany)] [Applied Functional Polymers, University of Bayreuth, 95440 Bayreuth (Germany)

2013-11-25T23:59:59.000Z

156

Combined single-molecule force and fluorescence measurements for biology  

E-Print Network [OSTI]

Minireview Combined single-molecule force and fluorescence measurements for biology Mark I Wallace, some of which allow cellular processes to be examined at the level of a single molecule. In particular of resolv- ing single molecules in aqueous solution at room tempera- ture [1-7]. Now that the field is over

Wallace, Mark

157

Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging  

Science Journals Connector (OSTI)

...research-article Methods Benchmarking Various Green Fluorescent Protein Variants in Bacillus...contributed equally to this article. Green fluorescent protein (GFP) offers efficient...INTRODUCTION The use of Aequorea victoria green fluorescent protein (GFP) and its derivatives...

Wout Overkamp; Katrin Beilharz; Ruud Detert Oude Weme; Ana Solopova; Harma Karsens; kos T. Kovcs; Jan Kok; Oscar P. Kuipers; Jan-Willem Veening

2013-08-16T23:59:59.000Z

158

Understanding Zinc Quantification with Existing and Advanced Ditopic Fluorescent Zinpyr Sensors  

E-Print Network [OSTI]

Treatment of aqueous zinc solutions with incremental additions of a ditopic fluorescent sensor of the Zinpyr family, based on pyridine/pyrazine-containing metal recognition units, affords a fluorescence titration curve ...

Buccella, Daniela

159

E-Print Network 3.0 - absolute fluorescence yield Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the fluorescence quantum yields, s and r... ) and 3(c) show the absolute TPE spectra for green- and red-fluorescence, respectively. The TPE spectra... Measurement of two-photon...

160

Gold Nanoparticle Enlargement Coupled with Fluorescence Quenching for Highly Sensitive Detection of Analytes  

Science Journals Connector (OSTI)

We report a versatile and facile route for highly sensitive detection of analytes through coupling the enlargement of gold nanoparticles with fluorescence quenching. The fluorescence intensity of dye molecules (e.g., fluorescein or rhodamine B) ...

Seong Yoon Lim; Jae Hong Kim; Joon Seok Lee; Chan Beum Park

2009-10-29T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Laser- and UV-LED-Induced Fluorescence Detection of Drinking Water and Water-Dissolved Organics  

Science Journals Connector (OSTI)

We have developed a deep-UV laser-induced fluorescence system for fluorescence detection of water-dissolved organic species. Deep-UV LEDs also were used as the excitation source....

Sharikova, Anna V; Killinger, Dennis K

162

Detection of Ethylene Gas by Fluorescence Turn-On of a Conjugated Polymer  

E-Print Network [OSTI]

Ripe fruits: The fluorescence of a conjugated polymer is quenched by the presence of copper(I) moieties. Upon exposure to ethylene gas the copper complexes bind to ethylene and no longer quench the polymer fluorescence ...

Swager, Timothy Manning

163

Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology  

E-Print Network [OSTI]

Fluorescence spectroscopy is a powerful tool for probing complex biological processes. The ubiquity of peptideprotein and proteinprotein interactions in these processes has made them important targets for fluorescence ...

Krueger, Andrew T.

164

Extending the utility of enzymes for site-specific targeting of fluorescent probes  

E-Print Network [OSTI]

Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, ...

Liu, Daniel S. (Daniel Shao-Chen)

2014-01-01T23:59:59.000Z

165

Fluorescence Resonance Energy Transfer Reveals a Binding Site of a Photosensitizer for Photodynamic Therapy  

Science Journals Connector (OSTI)

...D., Kenney M. E. New phthalocyanine photosensitizers...Stryer L. Fluorescence energy transfer as a spectroscopic...39-40, Marcel Dekker Inc. New York 1993. 20 Clegg R. M. Fluorescence resonance energy transfer. Curr. Opin...

Rachel L. Morris; Kashif Azizuddin; Minh Lam; Jeffrey Berlin; Anna-Liisa Nieminen; Malcolm E. Kenney; Anna C. S. Samia; Clemens Burda; and Nancy L. Oleinick

2003-09-01T23:59:59.000Z

166

Characterization of coal oil using three-dimensional excitation and emission matrix fluorescence spectroscopy  

Science Journals Connector (OSTI)

Three-dimensional (3D) excitation-emission matrix (EEM) fluorescence spectroscopy is applied to characterize the coal oil. The results show that the 3D fluorescence spectra of coal oil...

Xiao, Xue; Zhang, Yujun; Wang, Zhigang; Jin, Dan; Yin, Gaofang; Liu, Wenqing

2009-01-01T23:59:59.000Z

167

Seminaphthofluorescein-Based Fluorescent Probes for Imaging Nitric Oxide in Live Cells  

E-Print Network [OSTI]

Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic ...

Pluth, Michael D.

168

E-Print Network 3.0 - air fluorescence measurements Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

naphthalene with laser-induced fluorescence M. Martinez... and Physics Discussions Measuring ... Source: Ecole Polytechnique, Centre de mathmatiques Collection:...

169

DNA complexes with dyes designed for energy transfer as fluorescent markers  

DOE Patents [OSTI]

Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

Glazer, Alexander M. (Orinda, CA); Benson, Scott C. (Albany, CA)

1999-01-01T23:59:59.000Z

170

Real-time Fluorescent Resonance Energy Transfer Analysis to Monitor Drug Resistance in Chronic Myelogenous Leukemia  

Science Journals Connector (OSTI)

...NH2-terminal YFP (yellow mutant of green fluorescent protein) and a COOH-terminal CFP (cyan mutant of green fluorescent protein). However...conformation change and yielding an energy transfer from CFP to YFP. To...pair of fluorescent resonance energy transfer-based probes for...

Ahmet Tunceroglu; Michiyuki Matsuda; and Raymond B. Birge

2010-11-01T23:59:59.000Z

171

RAPID COMMUNICATION An Integral Membrane Green Fluorescent Protein Marker, Us9-GFP,  

E-Print Network [OSTI]

RAPID COMMUNICATION An Integral Membrane Green Fluorescent Protein Marker, Us9-GFP described GFP-spectrin, a membrane- localized derivative of the green fluorescent protein that can proteins employed in this assay. © 1999 Academic Press Key Words: green fluorescent protein; cell cycle

172

Fluorescence quenching of CdSe quantum dots on graphene  

SciTech Connect (OSTI)

We studied systematically the fluorescence quenching of CdSe quantum dots (QDs) on graphene and its multilayers, as well as graphene oxide (GO) and reduced graphene oxide (rGO). Raman intensity of QDs was used as a quantitatively measurement of its concentration in order to achieve a reliable quenching factor (QF). It was found that the QF of graphene (?13.1) and its multilayers is much larger than rGO (?4.4), while GO (?1.5) has the lowest quenching efficiency, which suggests that the graphitic structure is an important factor for quenching the fluorescence of QDs. It was also revealed that the QF of graphene is not strongly dependent on its thicknesses.

Guo, Xi Tao; Hua Ni, Zhen, E-mail: zhni@seu.edu.cn; Yan Nan, Hai; Hui Wang, Wen [Department of Physics and Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 211189 (China)] [Department of Physics and Key Laboratory of MEMS of the Ministry of Education, Southeast University, Nanjing 211189 (China); Yan Liao, Chun [Physics Department, National Photoelectric Technology and Functional Materials and Application of Science and Technology International Cooperation Base, Northwest University, Xi'an 710069 (China)] [Physics Department, National Photoelectric Technology and Functional Materials and Application of Science and Technology International Cooperation Base, Northwest University, Xi'an 710069 (China); Zhang, Yan; Wei Zhao, Wei [Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189 (China)] [Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189 (China)

2013-11-11T23:59:59.000Z

173

Blue fluorescent organic light emitting diodes with multilayered graphene anode  

SciTech Connect (OSTI)

As an innovative anode for organic light emitting devices (OLEDs), we have investigated graphene films. Graphene has importance due to its huge potential in flexible OLED applications. In this work, graphene films have been catalytically grown and transferred to the glass substrate for OLED fabrications. We have successfully fabricated 2 mm 2 mm device area blue fluorescent OLEDs with graphene anodes which showed 2.1% of external quantum efficiency at 1000 cd/m{sup 2}. This is the highest value reported among fluorescent OLEDs using graphene anodes. Oxygen plasma treatment on graphene has been found to improve hole injections in low voltage regime, which has been interpreted as oxygen plasma induced work function modification. However, plasma treatment also increases the sheet resistance of graphene, limiting the maximum luminance. In summary, our works demonstrate the practical possibility of graphene as an anode material for OLEDs and suggest a processing route which can be applied to various graphene related devices.

Hwang, Joohyun [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Choi, Hong Kyw [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Moon, Jaehyun; Shin, Jin-Wook; Joo, Chul Woong; Han, Jun-Han; Cho, Doo-Hee; Huh, Jin Woo [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Choi, Sung-Yool [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [Graphene Electronics Creative Research Section, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Lee, Jeong-Ik, E-mail: jiklee@etri.re.kr [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of); Chu, Hye Yong [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)] [OLED Lighting Research Team, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700 (Korea, Republic of)

2012-10-15T23:59:59.000Z

174

Nanoparticle sizing method based on fluorescence anisotropy analysis  

Science Journals Connector (OSTI)

Abstract Demand for applications of nanoparticles in electric architecture has been increasing. Nanoparticles provide new opportunities for improving circuit response. We discuss a novel method for evaluating nanoparticle sizes based on fluorescence anisotropy analysis. Particle size evaluation is possible through measurements of the rotational diffusion coefficient, which is sensitive to particle size. We develop a system for measuring rotational diffusion coefficients by using a fluorescent probe to label a particle. We report fundamental experiments that verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging 620nm were measured using the proposed method. The measured rotational diffusion coefficients decrease with increasing particle size. This finding indicates that nanoparticles smaller than 15nm can be measured with fine resolution.

Terutake Hayashi; Yuki Ishizaki; Masaki Michihata; Yasuhiro Takaya; Shin-Ichi Tanaka

2015-01-01T23:59:59.000Z

175

DNA fragment sizing and sorting by laser-induced fluorescence  

DOE Patents [OSTI]

A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)

1996-01-01T23:59:59.000Z

176

Structural basis for reversible photobleaching of a green fluorescent protein homologue  

SciTech Connect (OSTI)

Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.

Henderson, J. Nathan; Ai, Hui-wang; Campbell, Robert E.; Remington, S. James (Alberta); (Oregon)

2008-09-03T23:59:59.000Z

177

Microwave-Assisted Synthesis of Fluorescent Carbon Nanoparticles  

E-Print Network [OSTI]

MICROWAVE-ASSITED SYNTHESIS OF FLUORESCENT CARBON NANOPARTICLES A Thesis by BONNIE CHEN Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree...-(hydroxymethyl) furfural (5- HMF) derivatives, which are major products of glucose decomposition. iv ACKNOWLEDGEMENTS This thesis was completed in my fourth year at Texas A&M University as a master's student, and analyzes, discusses and displays...

Chen, Bonnie

2014-08-19T23:59:59.000Z

178

Nuclear Resonance Fluorescence and Isotopic Mapping of Containers  

SciTech Connect (OSTI)

National security programs have expressed interest in developing systems to isotopically map shipping containers, fuel assemblies, and waste barrels for various materials including special nuclear material (SNM). Current radiographic systems offer little more than an ambiguous density silhouette of a container's contents. In this paper we will present a system being developed at LLNL to isotopically map containers using the nuclear resonance fluorescence (NRF) method. Recent experimental measurements on NRF strengths in SNM are discussed.

Johnson, M S; McNabb, D P

2008-08-08T23:59:59.000Z

179

Development of a fluorescent antibody test for equine infectious anemia  

E-Print Network [OSTI]

. Moore The development of a direct fluorescent antibody technique for a reliable laboratory and diagnostic test for equine infectious anemia (EIA) was described. A specific antiserum was produced by inoculation of goats with EIA virus derived from... the Texas and Illinois strains of EIA virus in cell culture and in known infected horses and against the Wyoming strain of EIA virus in inoculated horses. Its specificity was further substantiated by testing it against heterologous viruses including...

Lester, Thomas Lee

1972-01-01T23:59:59.000Z

180

Dynamic fluorescence imaging with molecular agents for cancer detection  

E-Print Network [OSTI]

emission computed tomography SUV standard uptake values SLN sentinel lymph node TK tyrosine kinase TBR target to background ratio Symbols ? pharmacokinetic parameter ? pharmacokinetic parameter )(tC BOUND concentration of dye... bound to integrin receptor either in the vascular or extravascular compartments )(tC LAREXTRAVASCU concentration of dye in extravascular space )(tC VASCULAR concentration of dye in vascular space 0 I background fluorescence intensity c k...

Kwon, Sun Kuk

2009-05-15T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Magnetic fluorescent lamp having reduced ultraviolet self-absorption  

DOE Patents [OSTI]

The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly (10) is enhanced by providing means (30) for establishing a magnetic field with lines of force along the path of electron flow through the bulb (12) of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

Berman, Samuel M. (San Francisco, CA); Richardson, Robert W. (Pelham, NY)

1985-01-01T23:59:59.000Z

182

Operating temperatures of recessed fluorescent fixtures with thermal insulation  

SciTech Connect (OSTI)

Tests were performed to determine steady state surface temperatures for recessed fluorescent fixtures operated with and without thermal insulation on the top side of the fixture and to identify potential problems associated with the installation of thermal insulation. In addition to measuring temperatures, means were sought by which the fixtures can be thermally insulated and operated without fire hazards or damage to the fixture. (MCW)

Yarbrough, D.W.; Toor, I.A.

1981-05-01T23:59:59.000Z

183

Photon statistics of atomic fluorescence after {pi}-pulse excitation  

SciTech Connect (OSTI)

The photon statistics of atomic fluorescence after {pi}-pulse excitation is investigated in a system in which the input and output ports are connected to an atom. Since spontaneous decay during input pulse excitation occurs, the output pulse generally contains a multiphoton component with a certain probability. We quantitatively evaluate the probability of the output pulse containing multiple photons and determine the conditions for ideal single-photon generation.

Yoshimi, Kazuyoshi [College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827 (Japan); Koshino, Kazuki [College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827 (Japan); PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012 (Japan)

2010-09-15T23:59:59.000Z

184

Remediation plan for fluorescent light fixtures containing polychlorinated biphenyls (PCBs)  

SciTech Connect (OSTI)

This report describes the remedial action to achieve compliance with 29 CFR 1910 Occupational Safety and Health Administration (OSHA) requirements of fluorescent light fixtures containing PCBs at K-25 site. This remedial action is called the Remediation Plan for Fluorescent Light Fixtures Containing PCBs at the K-25 Site (The Plan). The Plan specifically discusses (1) conditions of non-compliance, (2) alternative solutions, (3) recommended solution, (4) remediation plan costs, (5) corrective action, (6) disposal of PCB waste, (7) training, and (8) plan conclusions. The results from inspections by Energy Systems personnel in 2 buildings at K-25 site and statistical extension of this data to 91 selected buildings at the K-25 site indicates that there are approximately 28,000 fluorescent light fixtures containing 47,036 ballasts. Approximately 38,531 contain PCBs and 2,799 of the 38,531 ballasts are leaking PCBs. Review of reportable occurrences at K-25 for the 12 month period of September 1990 through August 1991 shows that Energy Systems personnel reported 69 ballasts leaking PCBs. Each leaking ballast is in non-compliance with 29 CFR 1910 - Table Z-1-A. The age of the K-25 facilities indicate a continued and potential increase in ballasts leaking PCBs. This report considers 4 alternative solutions for dealing with the ballasts leaking PCBs. The advantages and disadvantages of each alternative solution are discussed and ranked using cost of remediation, reduction of health risks, and compliance with OSHA as criteria.

NONE

1992-04-30T23:59:59.000Z

185

ISSUANCE 2014-12-29: Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts  

Broader source: Energy.gov [DOE]

Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

186

The Energy Efficiency of the U.S Fluorescent Lamp Ballast Industry  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The Energy Efficiency of the U.S Fluorescent Lamp Ballast Industry The Energy Efficiency of the U.S Fluorescent Lamp Ballast Industry Speaker(s): Francis Rubinstein Date: February 14, 2008 - 12:00pm Location: 90-3122 Fluorescent lamp ballasts are subject to DOE appliance standards and rulemaking. But currently, only ballasts for older fluorescent lamp types, such as T-12 lamps are subject to minimum efficacy limits. The majority of fluorescent lamps now being shipped (T-8 and T-5 types) type are operated by ballasts that will not be subject to DOE rule until 2011. The metric used by DOE for characterizing the electrical efficiency of the fluorescent lamp-ballast system is called Ballast Efficacy Factor (BEF). Because BEF is not normalized, it is of limited utility for rulemaking and is useless for procurement. But by normalizing BEF to the total lamp power, I have

187

A light diet for a giant appetite: An assessment of China's proposed fluorescent lamp standard  

SciTech Connect (OSTI)

Lighting has been one of the fastest growing electric end-uses in China over the last twenty years, with an average annual growth rate of 14%. Fluorescent lighting provides a significant portion of China's lighting need. In 1998, China produced 680 million fluorescent lamps, of which 420 million were linear fluorescent lamps of various diameters (T8 to T12). There are substantial variations both in energy efficiency and lighting performance among locally produced fluorescent lamps. Such variations present a perfect opportunity for policy intervention through efficiency standards to promote the adoption of more efficient fluorescent lamps in China. This paper analyzes China's proposed minimum efficiency standard for fluorescent lamps and presents an assessment of its likely impacts on China's lighting energy consumption and GHG emissions.

Lin, Jiang

2002-04-11T23:59:59.000Z

188

X-ray fluorescence spectroscopy from ions at charged vapor/water interfaces  

E-Print Network [OSTI]

X-ray fluorescence spectra from monovalent ions (Cs+) that accumulate from dilute solutions to form an ion-rich layer near a charged Langmuir monolayer are presented. For the salt solution without the monolayer, the fluorescence signals below the critical angle are significantly lower than the detection sensitivity and only above the critical angle signals from the bulk are observed. In the presence of a monolayer that provides surface charges, strong fluorescence signals below the critical angle are observed. Ion density accumulated at the interface are determined from the fluorescence. The fluorescent spectra collected as a function of incident x-ray energy near the LIII edge yield the extended absorption spectra from the ions, and are compared to recent independent results. The fluorescence data from divalent Ba2+ with and without monolayer are also presented.

Wei Bu; David Vaknin

2009-05-04T23:59:59.000Z

189

Nanoscale fluorescence lifetime imaging with a single diamond NV center  

E-Print Network [OSTI]

Solid-state quantum emitters, such as artificially engineered quantum dots or naturally occurring defects in solids, are being investigated for applications ranging from quantum information science and optoelectronics to biomedical imaging. Recently, these same systems have also been studied from the perspective of nanoscale metrology. In this letter we study the near-field optical properties of a diamond nanocrystal hosting a single nitrogen vacancy center. We find that the nitrogen vacancy center is a sensitive probe of the surrounding electromagnetic mode structure. We exploit this sensitivity to demonstrate nanoscale fluorescence lifetime imaging microscopy (FLIM) with a single nitrogen vacancy center by imaging the local density of states of an optical antenna.

Ryan Beams; Dallas Smith; Timothy W. Johnson; Sang-Hyun Oh; Lukas Novotny; Nick Vamivakas

2013-03-05T23:59:59.000Z

190

Maintaining optimum fluorescent lamp performance under elevated temperature conditions  

SciTech Connect (OSTI)

This paper describes a new technique for optimizing fluorescent lamp performance under elevated temperature conditions. This approach uses a thermo-electric Peltier device to produce a localized cold spot temperature of approximately 40/sup 0/C, allowing the lamps to maintain maximum light output and efficacy independent of prevailing ambient temperatures inside a luminaire. Experimental data show that a 20% increase in light output and a 10% increase in efficacy over typical lamp performance in a warm fixture environment can be obtained using this device. Only 0.25 watts must be supplied to the Peltier device to produce these results.

Siminovitch, M.J.; Rubinstein, F.M.; Clark, T.A.; Verderber, R.R.

1986-04-01T23:59:59.000Z

191

Fluorescence Detector for Capillary Separations Fabricated by 3D Printing  

Science Journals Connector (OSTI)

Fluorescence Detector for Capillary Separations Fabricated by 3D Printing ... Additive manufacturing, also known as 3D printing, rapid prototyping, or solid-freeform technology, has become an efficient method for rapid prototyping and fabrication applications ranging from small home projects to architecture and industrial machining. ... Compared to the more expensive alternatives using the standard optic component, the 3D printing provides significant cost reduction and, especially, sharing of information between laboratories for easy replication or further modifications, customization of the spatial arrangement, and miniaturization. ...

Jan Prikryl; Frantisek Foret

2014-11-26T23:59:59.000Z

192

Microcavity effects on the generation,fluorescence, and diffusion of excitons in organic solar cells  

E-Print Network [OSTI]

We compute the short-circuit diffusion current of excitons in an organic solar cell, with special emphasis on fluorescence losses. The exciton diffusion length is not uniform but varies with its position within the device, even with moderate fluorescence quantum efficiency. With large quantum efficiencies, the rate of fluorescence can be strongly reduced with proper choices of the geometrical and dielectric parameters. In this way, the diffusion length can be increased and the device performance significantly improved.

Kozyreff, G; Vuong, L T; Silleras, O Nieto; Martorell, J

2013-01-01T23:59:59.000Z

193

Fluorescence of synthetic DNA's at room temperature and neutral pH  

Science Journals Connector (OSTI)

The fluorescence of two synthetic DNA's, polyd(m5C) and poly[d(I-m5C)] is demonstrated at room temperature and neutral pH. Polyd(m5C) at pH 8.0 exhibits fluorescence qualitatively the same as the mononucleotide: the quantum yield is independent of excitation energy; the emission maximum is at 2.92 ??1 (355 nm). Poly[d(I-m5C)] exhibits fluorescence resembling that of the 5-methyldeoxycytidine component with an additional feature that is probably due to weak deoxyinosine fluorescence. Neither of these synthetic DNA's exhibits spectra suggestive of exciplex formation.

James E. Gill

1971-01-01T23:59:59.000Z

194

Fluorescence spectrum analysis using Fourier series modeling for Fluorescein solution in Ethanol  

E-Print Network [OSTI]

We have measured the fluorescence spectrum for fluorescein solution in ethanol with concentration 1 {\\times} 10-3 mol/liter at different temperatures from room temperature to freezing point of solvent, (T = 153, 183, 223, 253, and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has been used to determine the fitting curve and fitting equation for each fluorescence spectrum. Fourier series (3 {\\times} 2) was the most suitable fitting equation for all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol at T = 183K was calculated and compared with experimental fluorescence spectrum at the same temperature. There is a good similarity between them.

Hadi, Mahasin F

2011-01-01T23:59:59.000Z

195

Giant fluorescence enhancement of molecules coupled to plasmonic nanoscale patch antennas  

Science Journals Connector (OSTI)

We demonstrate a colloidally synthesized and tunable plasmonic platform for giant fluorescence enhancement and increased spontaneous emission rate of embedded fluorophores. A...

Mikkelsen, Maiken H; Rose, Alec; Hoang, Thang B; McGuire, Felicia; Mock, Jack J; Ciraci, Cristian; Smith, David R

196

Plastic lab-on-a-chip for fluorescence excitation with integrated organic semiconductor lasers  

Science Journals Connector (OSTI)

Laser light excitation of fluorescent markers offers highly sensitive and specific analysis for bio-medical or chemical analysis. To profit from these advantages for applications in...

Vannahme, Christoph; Klinkhammer, Snke; Lemmer, Uli; Mappes, Timo

2011-01-01T23:59:59.000Z

197

Fluorescence from CsF ionic excimers excited by an electron beam  

SciTech Connect (OSTI)

Mixtures of rare gases and cesium fluoride vapors were excited by an intense relativistic electron beam. The fluorescence of the Cs[sup 2+]F[sup [minus

XYang, T.T.; Gylys, V.T.; Bower, R.D.; Harris, D.G. (Rocketdyne Division, Rockwell International, 6633 Canoga Avenue, Canoga Park, California 91303 (United States))

1992-08-01T23:59:59.000Z

198

Characterization of four potential laser-induced fluorescence tracers for diesel engine applications  

Science Journals Connector (OSTI)

Four potential laser-induced fluorescence (LIF) tracers, 1-phenyloctane, 1-phenyldecane, 1-methylnaphthalene, and 2-methylnaphthalene, are characterized for diesel engine applications....

Trost, Johannes; Zigan, Lars; Leipertz, Alfred; Sahoo, Dipankar; Miles, Paul C

2013-01-01T23:59:59.000Z

199

Nuclear Resonance Fluorescence to Measure Plutonium Mass in Spent Nuclear Fuel  

E-Print Network [OSTI]

and Nuclear Recoil . . . . . . . . . . . . . . . . . . . . .2 Quantitative Measurements using NRF 2.1 Nuclear ResonanceFuture Work A Transmission Nuclear Resonance Fluorescence

Ludewigt, Bernhard A

2011-01-01T23:59:59.000Z

200

Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging  

Science Journals Connector (OSTI)

We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of...

Solomon, Metasebya; White, Brian R; Nothdruft, Ralph E; Akers, Walter; Sudlow, Gail; Eggebrecht, Adam T; Achilefu, Samuel; Culver, Joseph P

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

High-frequency demodulation of multiphoton fluorescence in long-wavelength hyper-Rayleigh scattering  

Science Journals Connector (OSTI)

Suppression of the multiphoton fluorescence contribution to the hyper-Rayleigh (second-order nonlinear optical) scattering signal was recently achieved by intrinsic demodulation of...

Olbrechts, Geert; Wostyn, Kurt; Clays, Koen; Persoons, Andr

1999-01-01T23:59:59.000Z

202

Shelf life of five meat products displayed under light emitting diode or fluorescent lighting.  

E-Print Network [OSTI]

??Light emitting diode (LED) and fluorescent (FLS) lighting effects on enhanced pork loin chops, beef longissimus dorsi and semimembranosus steaks, ground beef, and ground turkey (more)

Steele, Kyle Stover

2011-01-01T23:59:59.000Z

203

Detection of gas-phase HCl by the laser-induced sensitized fluorescence of CO2  

Science Journals Connector (OSTI)

A detection system for measuring HCl in the gas phase by monitoring sensitized fluorescence following intracavity excitation by a HCl chemical laser is described. It is possible to...

Fernando, Rohantha P

1986-01-01T23:59:59.000Z

204

E-Print Network 3.0 - amplified fluorescent transduction Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2 PH.9 MICROSYSTEMS TECHNOLOGY LABORATORIES ANNUAL RESEARCH REPORT 2009 PHotonics Heterojunction Photoconductors for chemical Detection Summary: of a fluorescent polymer-chemical...

205

E-Print Network 3.0 - atomic fluorescence spectrometric Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of Selenium by Fluorescence Spectrometry Summary: MATERIALS 129 5. Ihnat. M.. Atomic absorption spectrometric determination of selenium with carbon furnace... I...

206

Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection of Small Molecules  

E-Print Network [OSTI]

Graphene Signal Amplification for Sensitive and Real-Time Fluorescence Anisotropy Detection graphene oxide (GO) as the signal amplifier. Because of the extraordinarily larger volume of GO

Tan, Weihong

207

Anatomy-Based Algorithms for Detecting Oral Cancer Using Reflectance and Fluorescence Spectroscopy  

E-Print Network [OSTI]

OBJECTIVES: We used reflectance and fluorescence spectroscopy to noninvasively and quantitatively distinguish benign from dysplastic/malignant oral lesions. We designed diagnostic algorithms to account for differences in ...

McGee, Sasha

208

Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization  

DOE Patents [OSTI]

Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

Jankowiak, R.J.; Small, G.J.; Shields, P.A.

1999-04-27T23:59:59.000Z

209

Molecular Fluorescence accompanying the Twilight Injection of Triethylborane into the Upper Atmosphere  

Science Journals Connector (OSTI)

... fluorescence spectrum, and at the same time to obtain data on the upper atmospheric \\vind from the motion of the released material.

J. M. HOFFMAN; M. A. PALMER; L. B. SMITH

1967-09-09T23:59:59.000Z

210

Cerenkov emission induced by external beam radiation stimulates molecular fluorescence  

SciTech Connect (OSTI)

Purpose: Cerenkov emission is induced when a charged particle moves faster than the speed of light in a given medium. Both x-ray photons and electrons produce optical Cerenkov photons in everyday radiation therapy of tissue; yet, this phenomenon has never been fully documented. This study quantifies the emissions and also demonstrates that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms. Methods: In this study, Cerenkov emission induced by radiation from a clinical linear accelerator is investigated. Biological mimicking phantoms were irradiated with x-ray photons, with energies of 6 or 18 MV, or electrons at energies 6, 9, 12, 15, or 18 MeV. The Cerenkov emission and the induced molecular fluorescence were detected by a camera or a spectrometer equipped with a fiber optic cable. Results: It is shown that both x-ray photons and electrons, at MeV energies, produce optical Cerenkov photons in tissue mimicking media. Furthermore, we demonstrate that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms. Conclusions: The results here indicate that molecular fluorescence monitoring during external beam radiotherapy is possible.

Axelsson, Johan; Davis, Scott C.; Gladstone, David J.; Pogue, Brian W. [Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755 (United States); Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire 03766 (United States); Thayer School of Engineering and Department of Physics and Astronomy, Dartmouth College, Hanover, New Hampshire 03755 (United States)

2011-07-15T23:59:59.000Z

211

Status of the Silicon Photomultiplier Telescope FAMOUS for the Fluorescence Detection of UHECRs  

E-Print Network [OSTI]

An established technique for the measurement of ultra-high-energy-cosmic-rays is the detection of the fluorescence light induced in the atmosphere of the Earth, by means of telescopes equipped with photomultiplier tubes. Silicon photomultipliers (SiPMs) promise an increase in the photon detection efficiency which outperforms conventional photomultiplier tubes. In combination with their compact package, a moderate bias voltage of several ten volt and single photon resolution, the use of SiPMs can improve the energy and spatial resolution of air fluorescence measurements, and lead to a gain in information on the primary particle. Though, drawbacks like a high dark-noise-rate and a strong temperature dependency have to be managed. FAMOUS is a refracting telescope prototype instrumented with 64 SiPMs of which the main optical element is a Fresnel lens of 549.7 mm diameter and 502.1 mm focal length. The sensitive area of the SiPMs is increased by a special light collection system consisting of Winston cones. The t...

Niggemann, Tim; Brogueira, Pedro; Bueno, Antonio; Eichler, Hans Michael; Ferreira, Miguel; Hebbeker, Thomas; Lauscher, Markus; Mendes, Lus; Middendorf, Lukas; Navas, Sergio; Peters, Christine; Pimenta, Mrio; Ruiz, Angel; Schumacher, Johannes; Stephan, Maurice

2015-01-01T23:59:59.000Z

212

Conservation Potential of Compact Fluorescent Lamps in India and Brazil  

E-Print Network [OSTI]

1988. The private sector in Brazil owned (in 1986) 40% ofElectricity Conservation in Brazil: Potential and Pro-electricity consumption in Brazil's residential sector was

Gadgil, A.J.

2008-01-01T23:59:59.000Z

213

Conservation Potential of Compact Fluorescent Lamps in India and Brazil  

E-Print Network [OSTI]

also additional benefits from avoided costs of environmentalpremium for India)) - (avoided annual cost of incandescents)electricity) + (avoided annual cost of incandescents) - (

Gadgil, A.J.

2008-01-01T23:59:59.000Z

214

Zero energy-storage ballast for compact fluorescent lamps  

DOE Patents [OSTI]

A CFL ballast includes complementary-type switching devices connected in series with their gates connected together at a control node. The switching devices supply a resonant tank circuit which is tuned to a frequency near, but slightly lower than, the resonant frequency of a resonant control circuit. As a result, the tank circuit restarts oscillations immediately following each zero crossing of the bus voltage. Such rapid restarts avoid undesirable flickering while maintaining the operational advantages and high efficacy of the CFL ballast. 4 figs.

Schultz, W.N.; Thomas, R.J.

1999-08-31T23:59:59.000Z

215

Conservation Potential of Compact Fluorescent Lamps in India and Brazil  

E-Print Network [OSTI]

1.7% of in- stalled hydroelectric capacity. Op. cit. ref [in Ig86) based on hydroelectric generation, and most of thethe still abundant hydroelectric potential of the country.

Gadgil, A.J.

2008-01-01T23:59:59.000Z

216

Energy Cost Calculator for Compact Fluorescent Lamps | Department...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

project. Modify the default values to suit your project requirements. Existing incandescent lamp wattage Watts Incandescent lamp cost dollars Incandescent lamp life 1000 hours...

217

Conservation Potential of Compact Fluorescent Lamps in India and Brazil  

E-Print Network [OSTI]

38 TWh, 10% of which was for incandescent lighting (Fig. 3).The electricity consumed in incandescent lighting can be300 and 400 million incandescent lamps in the country. Let

Gadgil, A.J.

2008-01-01T23:59:59.000Z

218

Optimized Magnetic Components Improve Efficiency of Compact Fluorescent Lamps  

E-Print Network [OSTI]

xtures (designed for incandescent bulbs) has allowed residential and small commercial electric customers of incandescent lamps and last 10 times longer [1]. High-frequency electronic ballasts are used to power CFLs

219

[16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions  

E-Print Network [OSTI]

[16) Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions By SANG-HYUN PARK and RONALD T. RAINES Introduction Green fluorescent protein (GFP) from the jellyfish Aequorea victoria. These properties include high thermal stability and resis- tance to detergents, organic solvents, and proteases

Raines, Ronald T.

220

Fluorescence diagnostics of oil pollution in coastal marine waters by use of artificial neural networks  

E-Print Network [OSTI]

marine waters with fluorescence spectroscopy and of using artificial neural networks for data interpre with an artificial neural network. The results demonstrate the possibility of estimating oil concentrationsFluorescence diagnostics of oil pollution in coastal marine waters by use of artificial neural

Oldenburg, Carl von Ossietzky Universität

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

The suppression of fluorescence peaks in energy-dispersive X-ray diffraction  

Science Journals Connector (OSTI)

It is shown experimentally that diffraction peaks which are normally obscured by fluorescence peaks in energy-dispersive X-ray diffraction can be revealed by tuning of the X-ray tube excitation voltage in order to suppress the fluorescence peaks.

Hansford, G.M.

2014-09-30T23:59:59.000Z

222

Matrix-Induced Intensity Fluctuations in the Fluorescence from Single Oligo(phenylenevinylene) Molecules  

E-Print Network [OSTI]

trajectories, spectral shape and position, and fluorescence lifetimes can be used to investigate the chemical and theoretical reports have demonstrated how a fluctuating dielectric nanoenvironment surrounding a polarizable factors on molecular conforma- tion.15,16 In all of these studies, the fluorescent probe molecule is used

Buratto, Steve

223

Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Nanoscopy  

E-Print Network [OSTI]

Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Biology, Yale University School of Medicine, New Haven, Connecticut ABSTRACT Far-red fluorescent proteins are required for deep-tissue and whole-animal imaging and multicolor labeling in the red wavelength range

Verkhusha, Vladislav V.

224

Red-emitting fluorescent Organic Light emitting Diodes with low sensitivity to self-quenching  

E-Print Network [OSTI]

Red-emitting fluorescent Organic Light emitting Diodes with low sensitivity to self-quenching S, France *E-mail : sebastien.forget@univ-paris13.fr Keywords: OLED, quenching, doping, red-emitting organic. We herein report on Organic Light-Emitting Diodes (OLEDs) based on a fluorescent amorphous red

Paris-Sud XI, Université de

225

Characterising nutrient-induced fluorescence transients (NIFTs) in nitrogen-stressed Chlorella emersonii (Chlorophyta)  

E-Print Network [OSTI]

Characterising nutrient-induced fluorescence transients (NIFTs) in nitrogen-stressed Chlorella nutrient- induced fluorescence transients (NIFTs) in nitrogen-stressed Chlorella emersonii (Chlorophyta-starvation in batch cultures of the freshwater chlorophyte Chlorella emersonii was followed and the NIFT responses

McCarthy, T.K.

226

428 BIOCHIMICAET BIOPHYSICAACTA pH CONTROL OF THE CHLOROPHYLL a FLUORESCENCE IN ALGAE  

E-Print Network [OSTI]

on the "slow" (min) time course of Chlorophyll a fluorescence yield in the green alga Chlorella pyrenoidosa and in the blue-green alga Anacystis nidulans. In Chlorella, the decay of fluorescence yield, in the I- to 5-rain transient (i.e. the P level) in suspensions of whole Chlorella cells, and influence in general, the kinetic

Govindjee

227

Structure-Dependent Fluorescence Efficiencies of Individual Single-Walled Carbon Nanotubes  

E-Print Network [OSTI]

1 Structure-Dependent Fluorescence Efficiencies of Individual Single-Walled Carbon Nanotubes Dmitri, F-33405 France AUTHOR EMAIL ADDRESS: weisman@rice.edu RECEIVED DATE #12;2 ABSTRACT: Single-nanotube fluorimetric intensities. KEYWORDS: SWNT, carbon nanotubes, fluorescence, microscopy, spectroscopy, emission

Boyer, Edmond

228

Nucleic acid encoding a self-assembling split-fluorescent protein system  

DOE Patents [OSTI]

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

Waldo, Geoffrey S; Cabantous, Stephanie

2014-04-01T23:59:59.000Z

229

FLUORESCENCE CHANGES IN PORPHYRIDIUM EXPOSED TO GREEN LIGHT OF DIFFERENT INTENSITY: A NEW EMISSION  

E-Print Network [OSTI]

FLUORESCENCE CHANGES IN PORPHYRIDIUM EXPOSED TO GREEN LIGHT OF DIFFERENT INTENSITY: A NEW EMISSION supposed to require two light reactions for the transfer of one hydrogen atom from water to carbon dioxide the existence of this second trap. With increase in intensity of green light, I,, the differential fluorescence

Govindjee

230

Characterization of Pyoverdinpss, the Fluorescent Siderophore Produced by Pseudomonas syringae pv. syringae  

Science Journals Connector (OSTI)

...pyoverdinpss (peak V) was yellow-green and fluorescent when iron was...0~~~ 1.0 . j 0 20 40 60 lime (mlii) FIG. 4. Uptake of...Structure of the yellow-green fluorescent peptide produced...system using iron- containing minerals to limit iron repletion. J...

Yvonne S. Cody; Dennis C. Gross

1987-05-01T23:59:59.000Z

231

Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby  

DOE Patents [OSTI]

The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.

Waldo, Geoffrey S. (Santa Fe, NM)

2007-09-18T23:59:59.000Z

232

Nucleic acid encoding a self-assembling split-fluorescent protein system  

DOE Patents [OSTI]

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

2011-06-07T23:59:59.000Z

233

Sensors and Actuators B 111112 (2005) 230241 Improvements in LED-based fluorescence analysis systems  

E-Print Network [OSTI]

Sensors and Actuators B 111­112 (2005) 230­241 Improvements in LED-based fluorescence analysis the stability, power output, and spectral flexibility of light emitting diode (LED)-based systems used to excite fluorescence or other forms of luminescence. LEDs are an attractive alternative to conventional white

Wilson, Denise

234

Arrays and Cascades of Fluorescent Liquid-Liquid Waveguides: Broadband Light Sources for  

E-Print Network [OSTI]

Arrays and Cascades of Fluorescent Liquid-Liquid Waveguides: Broadband Light Sources) microchannel waveguides with liquid cores containing fluorescent dyes, excited by incident light from an external halogen bulb. Simultaneous use of multiple fluorophores in a common solution, in a single L2 light

Prentiss, Mara

235

Theoretical study of single molecule fluorescence in a metallic nanocavity Jorg Enderleina)  

E-Print Network [OSTI]

Theoretical study of single molecule fluorescence in a metallic nanocavity Jo¨rg Enderleina the metal. For the sake of sim- plicity, a single dye molecule centered within a dielectric bead coated on the fluorescence excitation and emission of a molecule embedded within a nanometric metal-coated dielectric bead

Enderlein, Jörg

236

Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna  

E-Print Network [OSTI]

Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna Anika Kinkhabwala1 mismatch between light and nanoscale objects such as single molecules, it is important to be able-contrast selection of single nanoemitters. A single fluorescent molecule (SM) with transition dipole m acts

Fan, Shanhui

237

High-throughput single-molecule fluorescence spectroscopy using parallel detection  

E-Print Network [OSTI]

High-throughput single-molecule fluorescence spectroscopy using parallel detection X. Michalet Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach in rare burst-like events corresponding to the transit of a single-molecule. Those events are accumulated

Michalet, Xavier

238

arXiv:astro-ph/0510375v112Oct2005 Comparison of air fluorescence and ionization  

E-Print Network [OSTI]

is maintained similar in shape in all showers, independent of initial energy, aside from the relatively sparsely, Salt Lake City, UT 84112, U.S.A. P. Chen, C. Field , C. Hast, R. Iverson, J.S.T. Ng, A. Odian, K. Reil fluorescence profiles in measuring ultra high energy cosmic rays. Key words: air fluorescence, electromagnetic

239

Near-Infrared Fluorescent pH-Sensitive Probes via Unexpected Barbituric Acid  

E-Print Network [OSTI]

Near-Infrared Fluorescent pH-Sensitive Probes via Unexpected Barbituric Acid Mediated Synthesis achilefus@mir.wustl.edu Received October 13, 2008 ABSTRACT Novel near-infrared pyrimidine-fused p by 2D-NMR. All new compounds are highly fluorescent in the near-infrared region and possess excellent

Larson-Prior, Linda

240

Targeting Beta-3 Integrin Using a Linear Hexapeptide Labeled with a Near-Infrared Fluorescent Molecular  

E-Print Network [OSTI]

Targeting Beta-3 Integrin Using a Linear Hexapeptide Labeled with a Near-Infrared Fluorescent-peptides labeled with a near-infrared fluorescent probe (cypate) showed that rearranging the glycine in a linear, and physiopathologic processes mediated by this protein. Keywords: Integrin; cancer; near-infrared; imaging; molecular

Larson-Prior, Linda

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Performance dependence of hybrid x-ray computed tomography/fluorescence molecular  

E-Print Network [OSTI]

Performance dependence of hybrid x-ray computed tomography/fluorescence molecular tomography imaging systems combining x-ray computed tomography (CT) and fluorescence tomography can im- prove Ntziachristos2 1 Department of Electrical and Computer Engineering, Northeastern University, 360 Huntington Ave

Miller, Eric

242

Optical fiber-based fluorescent viscosity sensor Mark A. Haidekker and Walter J. Akers  

E-Print Network [OSTI]

Optical fiber-based fluorescent viscosity sensor Mark A. Haidekker and Walter J. Akers Department bound to a fiber-optic tip without loss of viscosity sensi- tivity. The optical fiber itself may be used to molecular rotors in solution. An optical fiber-based fluorescent vis- cosity sensor may be used in real

Theodorakis, Emmanuel

243

X-ray Fluorescence Measurements of Manganese in Petroglyphs and Graffiti in the Bluff, Utah Area  

E-Print Network [OSTI]

X-ray Fluorescence Measurements of Manganese in Petroglyphs and Graffiti in the Bluff, Utah Area the age of rock art using Mn levels, Lytle (2008). In this work we use x-ray fluorescence (XRF) to measure of methods including atomic mass spectroscopy (AMS) measurements of 14 C, Particle-induced X-ray Excitation

244

Fluorescent Functionalized Mesoporous Silica for Radioactive Material Extraction  

SciTech Connect (OSTI)

Mesoporous silica with covalently bound salicylic acid molecules incorporated in the structure was synthesized with a one-pot, co-condensation reaction at room temperature. The as-synthesized material has a large surface area, uniform particle size, and an ordered pore structure as determined by characterization with transmission electron microscopy, thermal gravimetric analysis, and infrared spectra, etc. Using the strong fluorescence and metal coordination capability of salicylic acid, functionalized mesoporous silica (FMS) was developed to track and extract radionuclide contaminants, such as uranyl [U(VI)] ions encountered in subsurface environments. Adsorption measurements showed a strong affinity of the FMS toward U(VI) with a Kd value of 105 mL/g, which is four orders of magnitude higher than the adsorption of U(VI) onto most of the sediments in natural environments. The new materials have a potential for synergistic environmental monitoring and remediation of the radionuclide U(VI) from contaminated subsurface environments.

Li, Juan; Zhu, Kake; Shang, Jianying; Wang, Donghai; Nie, Zimin; Guo, Ruisong; Liu, Chongxuan; Wang, Zheming; Li, Xiaolin; Liu, Jun

2012-08-01T23:59:59.000Z

245

Doppler-free Yb spectroscopy with the fluorescence spot technique  

SciTech Connect (OSTI)

We demonstrate a simple technique to measure the resonant frequency of the 398.9-nm {sup 1}S{sub 0}{leftrightarrow}{sup 1}P{sub 1} transition for the different Yb isotopes. The technique, which works by observing and aligning fluorescence spots, has enabled us to measure transition frequencies and isotope shifts with an accuracy of 60 MHz. We provide wavelength measurements for the transition that differ from previously published work. Our technique also allows for the determination of Doppler-shifted transition frequencies for photoionization experiments when the atomic beam and the laser beam are not perpendicular and furthermore allows us to determine the average velocity of the atoms along the direction of the atomic beam.

Nizamani, Altaf H.; McLoughlin, James J.; Hensinger, Winfried K. [Department of Physics and Astronomy, University of Sussex, Falmer, Brighton, East-Sussex, BN1 9QH (United Kingdom)

2010-10-15T23:59:59.000Z

246

Hand-held X-Ray Fluorescence (XRF) | Open Energy Information  

Open Energy Info (EERE)

Hand-held X-Ray Fluorescence (XRF) Hand-held X-Ray Fluorescence (XRF) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Technique: Hand-held X-Ray Fluorescence (XRF) Details Activities (0) Areas (0) Regions (0) NEPA(0) Exploration Technique Information Exploration Group: Field Techniques Exploration Sub Group: Data Collection and Mapping Parent Exploration Technique: Data Collection and Mapping Information Provided by Technique Lithology: Bulk and trace element analysis of rocks, minerals, and sediments. Stratigraphic/Structural: Hydrological: Thermal: Dictionary.png Hand-held X-Ray Fluorescence (XRF): Hand-held X-Ray Fluorescence is a portable analytical technique derived from the instrumentation used in traditional lab-based XRF analysis. The technique is used for bulk chemical analysis of rock, mineral, and sediment

247

Electrochemically Controlled Surface Plasmon Enhanced Fluorescence Response of Surface Immobilized CdZnSe Quantum Dots  

Science Journals Connector (OSTI)

The application of negative potentials (versus Ag/AgCl) led to a decrease in the surface plasmon enhanced fluorescence signal, the fluorescence signal recovered if the cell potential was returned to 0 V or open circuit. ... UV?vis spectroscopy was carried out on a Perkin-Elmer Lambda 9 UV/vis/NIR spectrophotometer and fluorescence spectroscopy on a J&M TIDAS fluorescence spectrometer with illumination from a 100 W xenon bulb. ... In the research described in this paper, we show that such electrode potential control of QD fluorescence can also be achieved in an aqueous environment by close control of coupling conditions and QD chemistry leading to the possibility of using such structures for enhanced detection of biological and biorelated molecules. ...

Petra J. Cameron; Xinhua Zhong; Wolfgang Knoll

2009-03-23T23:59:59.000Z

248

An endogenous green fluorescent proteinphotoprotein pair in Clytia hemisphaerica eggs shows co-targeting to mitochondria and efficient bioluminescence energy transfer  

Science Journals Connector (OSTI)

...compartmentalization. green fluorescent protein...bioluminescence resonance energy transfer|clytin...chemiluminescence-resonance-energy-transfer chimaeras between green fluorescent protein...Cormier. 1979 An energy transfer protein...of the Renilla green-fluorescent protein...

2014-01-01T23:59:59.000Z

249

Dual-Emission Fluorescence Resonance Energy Transfer (FRET) Real-Time PCR Differentiates Feline Immunodeficiency Virus Subtypes and Discriminates Infected from Vaccinated Cats  

Science Journals Connector (OSTI)

...fluorescence resonance energy transfer (FRET...indicator of infection status in vaccinated cats...fluorescence resonance energy transfer (FRET...view/xhtml/en_us/smallanimal/inhouse...fluorescence resonance energy transfer (FRET...indicator of infection status. After the introduction...

Chengming Wang; Calvin M. Johnson; Sudhir K. Ahluwalia; Erfan Chowdhury; Yihang Li; Dongya Gao; Anil Poudel; K. Shamsur Rahman; Bernhard Kaltenboeck

2010-03-24T23:59:59.000Z

250

Application of a ratiometric laser induced fluorescence (LIF) thermometry for micro-scale temperature measurement for natural convection flows  

E-Print Network [OSTI]

A ratiometric laser induced fluorescence (LIF) thermometry applied to micro-scale temperature measurement for natural convection flows. To eliminate incident light non-uniformity and imperfection of recording device, two fluorescence dyes are used...

Lee, Heon Ju

2004-11-15T23:59:59.000Z

251

Single particle size and fluorescence spectra from emissions of burning materials in a tube furnace to simulate burn pits  

Science Journals Connector (OSTI)

A single-particle fluorescence spectrometer (SPFS) and an aerodynamic particle sizer were used to measure the fluorescence spectra and particle size distribution from the particulate emissions of 12 different burning

Yong-Le Pan; Joshua D. T. Houck; Pamela A. Clark; Ronald G. Pinnick

2013-08-01T23:59:59.000Z

252

The evaluation of ERK1 dimerization models using fluorescence correlation Ssectroscopy and the development of analysis algorithms for single-molecule super resolution.  

E-Print Network [OSTI]

??In this dissertation, we describe three advancements in the area of fluorescence spectroscopy and microscopy. First, we have implemented Fluorescence Correlation Spectroscopy on a home-built (more)

Huang, Fang, 1982-

2012-01-01T23:59:59.000Z

253

In Vivo Self-Assembly of Stable Green Fluorescent Protein Fusion Particles and Their Uses in Enzyme Immobilization  

Science Journals Connector (OSTI)

...Engineering In Vivo Self-Assembly of Stable Green Fluorescent Protein Fusion Particles and...previously the in vivo self-assembly of green fluorescent protein (GFP) fusions into...the design and recombinant production of green fluorescent protein (GFP) particles were...

Mark Venning-Slater; David O. Hooks; Bernd H. A. Rehm

2014-03-07T23:59:59.000Z

254

Comment on ``Resonance-fluorescence and absorption spectra of a two-level atom driven by a strong bichromatic field''  

E-Print Network [OSTI]

Comment on ``Resonance-fluorescence and absorption spectra of a two-level atom driven by a strong predictions of the resonance fluorescence from a two-level atom driven by a strong bichromatic field J. Opt.50.Hz, 33.50.Dq, 32.80. t In studying the resonance fluorescence from a driven two- level atom, we

Boyd, Robert W.

255

Denoising fluorescence endoscopy: a motion compensated temporal recursive video filter with an optimal minimum mean square error parameterization  

Science Journals Connector (OSTI)

Fluorescence endoscopy is an emerging technique for the detection of bladder cancer. A marker substance is brought into the patient's bladder which accumulates at cancer tissue. If a suitable narrow band light source is used for illumination, a red fluorescence ... Keywords: bladder, endoscopy, fluorescence, noise filtering, optimal filter, photo dynamic diagnostics

Thomas Stehle; Jonas Wulff; Alexander Behrens; Sebastian Gross; Til Aach

2009-06-01T23:59:59.000Z

256

Measurements of the Ultraviolet Fluorescence Cross Sections and Spectra of Bacillus Anthracis Simulants  

SciTech Connect (OSTI)

Measurements of the ultraviolet autofluorescence spectra and absolute cross sections of the Bacillus anthracis (Ba) simulants Bacillus globigii (Bg), Bacillus megaterium (Bm), Bacillus subtilis (Bs), and Bacillus cereus (Bc) were measured. Fluorescence spectra and cross sections of pine pollen (Pina echinata) were measured for comparison. Both dried vegetative cells and spores separated from the sporulated vegetative material were studied. The spectra were obtained by suspending a small number (<10) of particles in air in our Single Particle Spectroscopy Apparatus (SPSA), illuminating the particles with light from a spectrally filtered arc lamp, and measuring the fluorescence spectra of the particles. The illumination was 280 nm (20 nm FWHM) and the fluorescence spectra was measured between 300 and 450 nm. The fluorescence cross section of vegetative Bg peaks at 320 nm with a maximum cross section of 5 X 10{sup -14} cm{sup 2}/sr-nm-particle while the Bg spore fluorescence peaks at 310 nm with peak fluorescence of 8 X 10{sup -15} cm{sup 2}/sr-nm-particle. Pine pollen particles showed a higher fluorescence peaking at 355 nm with a cross section of 1.7 X 10{sup -13} cm{sup 2}/sr-nm-particle. Integrated cross sections ranged from 3.0 X 10{sup -13} for the Bg spores through 2.25 X 10{sup -12} (cm{sup 2}/sr-particle) for the vegetative cells.

Stephens, J.R.

1998-09-01T23:59:59.000Z

257

Cell-Trappable Fluorescent Probes for Nitric Oxide Visualization in Living Cells  

Science Journals Connector (OSTI)

(2) More recently, NO has been implicated to modulate synaptic activity in the CNS, where it may play a role in signal transduction in the olfactory bulb. ... Using the copper stoichiometry determined by titration, exposure of solutions of the probes generated in situ to excess NO under anaerobic conditions led to an immediate fluorescence enhancement with a concomitant bathochromic shift of the emission maxima (Figure 2 and Table 1). ... To test this presumption, fluorescent protein chimeras were expressed in RAW 264.7 macrophages, and time-lapse ratiometric fluorescence microscopy was used to measure the maturation dynamics of individual phagosomes contg. ...

Michael D. Pluth; Lindsey E. McQuade; Stephen J. Lippard

2010-04-20T23:59:59.000Z

258

High sensitivity fluorescent single particle and single molecule detection apparatus and method  

DOE Patents [OSTI]

Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.

Mathies, Richard A. (Contra Costa County, CA); Peck, Konan (Contra Costa County, CA); Stryer, Lubert (Santa Clara County, CA)

1990-01-01T23:59:59.000Z

259

Nuclear Resonance Fluorescence for Material Verification in Dismantlement  

SciTech Connect (OSTI)

Nuclear resonance fluorescence (NRF) is a well-established physical process that provides an isotope-specific signature that can be exploited for isotopic detection and characterization of samples. Pacific Northwest National Laboratory has been investigating possible applications of NRF for national security. Of the investigated applications, the verification of material in the dismantlement process is the most promising. Through a combination of benchmarking measurements and radiation transport modeling, we have shown that NRF techniques with existing bremsstrahlung photon sources and a modest detection system can be used to detect highly enriched uranium in the quantities and time limits relevant to the dismantlement process. Issues such as orientation, placement and material geometry do not significantly impact the sensitivity of the technique. We have also investigated how shielding of the uranium would be observed through non-NRF processes to enable the accurate assay of the material. This paper will discuss our findings on how NRF and photon-interrogation techniques may be applied to the material verification in the dismantlement process.

Warren, Glen A.; Detwiler, Rebecca S.

2011-10-01T23:59:59.000Z

260

Fluorescence from a quantum dot and metallic nanosphere hybrid system  

SciTech Connect (OSTI)

We present energy absorption and interference in a quantum dot-metallic nanosphere system embedded on a dielectric substrate. A control field is applied to induce dipole moments in the nanosphere and the quantum dot, and a probe field is applied to monitor absorption. Dipole moments in the quantum dot or the metal nanosphere are induced, both by the external fields and by each other's dipole fields. Thus, in addition to direct polarization, the metal nanosphere and the quantum dot will sense one another via the dipole-dipole interaction. The density matrix method was used to show that the absorption spectrum can be split from one peak to two peaks by the control field, and this can also be done by placing the metal sphere close to the quantum dot. When the two are extremely close together, a self-interaction in the quantum dot produces an asymmetry in the absorption peaks. In addition, the fluorescence efficiency can be quenched by the addition of a metal nanosphere. This hybrid system could be used to create ultra-fast switching and sensing nanodevices.

Schindel, Daniel G. [Department of Mathematics and Statistics, University of Winnipeg, 515 Portage Avenue, Winnipeg, MB, R3B 2E9 (Canada); Singh, Mahi R. [Department of Physics and Astronomy, University of Western Ontario, 1151 Richmond Street, London, ON, N6A 3K7 (Canada)

2014-03-31T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Nuclear Resonance Fluorescence at MIT | U.S. DOE Office of Science (SC)  

Office of Science (SC) Website

Resonance Fluorescence at MIT Resonance Fluorescence at MIT Nuclear Physics (NP) NP Home About Research Facilities Science Highlights Benefits of NP Spinoff Applications Spinoff Archives SBIR/STTR Applications of Nuclear Science and Technology Funding Opportunities Nuclear Science Advisory Committee (NSAC) News & Resources Contact Information Nuclear Physics U.S. Department of Energy SC-26/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3613 F: (301) 903-3833 E: sc.np@science.doe.gov More Information » Spinoff Archives Nuclear Resonance Fluorescence at MIT Print Text Size: A A A RSS Feeds FeedbackShare Page Application/instrumentation: Material Identification and Object Imaging Using Nuclear Resonance Fluorescence Developed at: Massachusetts Institute of Technology

262

Efficiency and concentration ratio measurements of fluorescent solar concentrators using a xenon measurement system  

Science Journals Connector (OSTI)

An indoor test stand for fluorescent planar concentratorcollectors (FPC) with a 1.6-kW xenon light source, irradiating a rectangular triangle (active area 400 cm2) with 82...

Heidler, K

1981-01-01T23:59:59.000Z

263

Application of Computers in Electron Probe and X-Ray Fluorescence Analysis  

Science Journals Connector (OSTI)

This paper is a review of automation of electron microprobe and x-ray fluorescence instrumentation. Such a review seems timely because of the great increase in the application of computer systems in this field...

Stanley D. Rasberry

1972-01-01T23:59:59.000Z

264

Frost Resistance of Wheat and CHL a in vivo Fluorescence Induction Kinetics  

Science Journals Connector (OSTI)

In recent years more and more studies have shown that physiological status of a living plant under different environmental conditions can be revealed by signals of chlorophyll a in vivo fluorescence induction ...

Shiqing Lin; Dianan Yang; Jide Zhang; Tongzhu Li

1990-01-01T23:59:59.000Z

265

Two-photon nitric oxide laser-induced fluorescence measurements in a diesel engine  

Science Journals Connector (OSTI)

A two-photon nitric oxide (NO) laser-induced fluorescence (LIF) technique was developed and applied to study in-cylinder diesel combustion. The technique prevents many problems...

Martin, Glen C; Mueller, Charles J; Lee, Chia-Fon F

2006-01-01T23:59:59.000Z

266

Detection of Thiobacillus ferrooxidans in acid mine environments by indirect fluorescent antibody staining.  

Science Journals Connector (OSTI)

...Industrial Waste | Coal Mining Epitopes Fluorescent...materials associated with coal. J. Bacteriol. 117...Effects of surface mining on fish and wildlife in Appalachia. Report, U.S...plasma acidophilum in coal refuse material. Appl...

W A Apel; P R Dugan; J A Filppi; M S Rheins

1976-07-01T23:59:59.000Z

267

Fluorescence of curium(III) in solutions of isopoly- and heteropolytungstates  

SciTech Connect (OSTI)

The authors describe the fluorescence of polytungstate complexes of curium (the term polytungstate complexes connotes both curium decatungstate and curium complexes with heteropolytungstate anions). The emitter is the curium(III) ion, which is excited as a result of energy transfer with the ligand. The data obtained permit a supplementation of the picture of the mutual interaction of the spectral and chemical properties of the f-elements with their ability to fluoresce in polytungstate complexes (there is only individual information on this question, pertaining only to the fluorescence of the lanthanides, chiefly Eu, Tb, Sm, and Dy). The fluorescence of curium(III) has also been used as a method of studying its complex formation with heteropolytungstate anions.

Yusov, A.B.; Fedoseev, A.M.; Spitsyn, V.I.; Krot, N.N.

1986-11-01T23:59:59.000Z

268

Turn-on fluorescent probes for detecting nitric oxide in biology  

E-Print Network [OSTI]

Chapter 1. Investigating the Biological Roles of Nitric Oxide and Other Reactive Nitrogen Species Using Fluorescent Probes: This chapter presents an overview of recent progress in the field of reactive nitrogen species ...

McQuade, Lindsey Elizabeth, 1981-

2010-01-01T23:59:59.000Z

269

Microcavity effects on the generation, fluorescence, and diffusion of excitons in organic solar cells  

Science Journals Connector (OSTI)

We compute the short-circuit diffusion current of excitons in an organic solar cell, with special emphasis on fluorescence losses. The exciton diffusion length is not uniform but...

Kozyreff, G; Urbanek, D C; Vuong, L T; Silleras, O Nieto; Martorell, J

2013-01-01T23:59:59.000Z

270

Optimization of Two-photon Excited Fluorescence Enhancement between Tunable and Broadband Femtosecond Laser Pulse Excitations  

E-Print Network [OSTI]

This project explores optimization of two-photon excited fluorescence (TPEF) enhancement between tunable narrowband and un-tuned broadband femtosecond (fs) laser pulse excitations for two-photon microscopy (TPM). The research is conducted...

Wang, Chao

2012-02-14T23:59:59.000Z

271

Development of a radiative transport based, fluorescence-enhanced, frequency-domain small animal imaging system  

E-Print Network [OSTI]

% intralipid solutions. Through collaboration with Transpire, Inc., a fluorescence-enhanced, frequency-domain, radiative transport equation (RTE) solver was developed. This solver incorporates the discrete ordinates, source iteration with diffusion synthetic...

Rasmussen, John C.

2009-05-15T23:59:59.000Z

272

Assessment of LED fluorescence microscopy for the diagnosis of Plasmodium falciparum infections in Gabon  

Science Journals Connector (OSTI)

LedFM and conventional fluorescence microscopy (uvFM) were compared to LM in 210 samples from patients with history of fever in the last 24 hours admitted to the Albert Schweitzer Hospital in Lambarn, Gabon.

Dominic Lenz; Peter G Kremsner; Bertrand Lell; Barbara Biallas

2011-07-01T23:59:59.000Z

273

In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes  

E-Print Network [OSTI]

Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although ...

Iverson, Nicole M.

274

Development of nanoparticle sizing system integrated with optical microscopy using fluorescence polarisation  

Science Journals Connector (OSTI)

There is a strong demand for the development of functional nanostructured devices. On the basis of fundamental chemistry, the fields of biotechnology and materials science have developed over the past three decades into powerful disciplines that allow the engineering of advanced technical devices. The functions of nanostructures are greatly influenced by their size and shape. In order to enable precise fabrication of nanostructured devices by bottom-up methods, real-time control based on in situ evaluation of nanoparticle growth and aggregation is needed. The fluorescence polarisation technique allows estimation of the size of a macromolecular structure from rotational diffusion constants, which can be obtained from the correlation of polarisation in fluorescence from fluorophores attached to the macromolecular structure. By analysing the degree of fluorescence polarisation, the theoretical size can be calculated by the Perrin-Weber formula. In this study, we investigated the degree of fluorescence polarisation dependency on different sizes of fluorescein-labelled nanoparticles.

Terutake Hayashi; Yasuhiro Takaya; Masaki Michihata

2012-01-01T23:59:59.000Z

275

On the origin of a slowly reversible fluorescence decay component in the Arabidopsis npq4 mutant  

Science Journals Connector (OSTI)

...regulation of photosynthetic energy conversion. Plant Cell 10, 1121-1134...photon absorption, excess energy dissipation and zeaxanthin...fluorescence. In Light as an energy source and information carrier in plant physiology (ed...

2014-01-01T23:59:59.000Z

276

AUTOMATED DETERMINATION OF PROTEIN SUBCELLULAR LOCATIONS FROM 3D FLUORESCENCE MICROSCOPE IMAGES  

E-Print Network [OSTI]

features (termed SLF for Subcellular Location Features) computed from 2D fluorescence microscope images [4]. We have shown the SLF to accurately represent the complexity in such images by using them

Gordon, Geoffrey J.

277

Cell-Trappable Fluorescent Probes for Nitric Oxide Visualization in Living Cells  

E-Print Network [OSTI]

Two new cell-trappable fluorescent probes for nitric oxide (NO) are reported based on either incorporation of hydrolyzable esters or conjugation to aminodextran polymers. Both probes are highly selective for NO over other ...

Pluth, Michael D.

278

E-Print Network 3.0 - anomalous x-ray fluorescence Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

in the emission of a fluorescent X-ray. As a rule of thumb 15... Optimization of X-ray energy resolution from a horizontally focused ... Source: Hendrickson, Wayne A. -...

279

Design of a New Fluorescent Cofactor for DNA Methyltransferases and Sequence-Specific Labeling of DNA  

Science Journals Connector (OSTI)

Sequence-specific labeling of DNA is of immense interest for analytical and functional studies of DNA. We present a novel approach for sequence-specific labeling of DNA using a newly designed fluorescent cofactor for the DNA methyltransferase from ...

Goran Pljevaljcic; Marc Pignot; Elmar Weinhold

2003-02-26T23:59:59.000Z

280

Toward whole-body optical imaging of rats using single-photon counting fluorescence tomography  

Science Journals Connector (OSTI)

We used single-photon counting (SPC) detection for diffuse fluorescence tomography to image nanomolar (nM) concentrations of reporter dyes through a rat. Detailed phantom data are...

Leblond, Frederic; Tichauer, Kenneth M; Holt, Robert W; El-Ghussein, Fadi; Pogue, Brian W

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Peptide Secondary Structure Modulates Single-Walled Carbon Nanotube Fluorescence as a Chaperone Sensor for Nitroaromatics  

E-Print Network [OSTI]

A class of peptides from the bombolitin family, not previously identified for nitroaromatic recognition, allows near-infrared fluorescent single-walled carbon nanotubes to transduce specific changes in their conformation. ...

Heller, Daniel Alan

282

Impact of Varying Atmospheric Profiles on Extensive Air Shower Observation: Fluorescence Light Emission and Energy Reconstruction  

E-Print Network [OSTI]

Several experiments measure the fluorescence light produced by extensive air showers in the atmosphere. This light is converted into a longitudinal shower profile from which information on the primary energy and composition is derived. The fluorescence yield, as the conversion factor between light profile measured by EAS experiments and physical interpretation of showers, has been measured in several laboratory experiments. The results, however, differ considerably. In this article, a model calculation of the fluorescence emission from relevant band systems of nitrogen in dependence on wavelength and atmospheric conditions is presented. Different calculations are compared to each other in combination with varying input parameters. The predictions are compared with measurements and the altitude-dependence of the fluorescence yield is discussed in detail.

B. Keilhauer; J. Bluemer; R. Engel; H. O. Klages

2005-11-05T23:59:59.000Z

283

Experimental study on the fluorescence lifetime of ethanol-water mixtures  

Science Journals Connector (OSTI)

The ethanol-water mixtures can emit fluorescence when excited by the ultraviolet (UV) light, which is different from pure ethanol and water. There are three emission bands of the...

Liu, Ying; Li, Rongqing; Lan, Xiufeng; Shen, Zhonghua; Lu, Jian; Ni, Xiaowu

2005-01-01T23:59:59.000Z

284

Fluorescent Dye Encapsulated ZnO Particles with Cell-specific...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Dye Encapsulated ZnO Particles with Cell-specific Toxicity for Potential use in Biomedical Applications. Fluorescent Dye Encapsulated ZnO Particles with Cell-specific Toxicity...

285

BIOCHIMICA ET BIOPHYSICA ACTA 371 FLUORESCENCE SPECTRA OF CHLORELLA IN THE 295-77K RANGE*  

E-Print Network [OSTI]

BIOCHIMICA ET BIOPHYSICA ACTA 371 BBA45950 FLUORESCENCE SPECTRA OF CHLORELLA IN THE 295-77°K RANGE A relation of fluorescence spectra of Chlorella (in the 295-77°K range) to the changes in the phase in the 77-295°K range, is composed of several bands (F7I7 and F725). If Chlorella cells are first cooled

Govindjee

286

Laser induced coal fluorescence. Ninth quarterly technical progress report, October 1-December 31, 1984. [Alginite  

SciTech Connect (OSTI)

The work during this reporting period has included a systematic fluorescence analysis of thirteen New Albany Shale samples of the Illinois basin. Fluorescence spectra obtained by continuous wave (c-w) excitation of 45 alginite macerals were collected for each shale sample. This work is now being continued by collecting the time-resolved data, and preliminary results are reported here. Additional software has been written to simplify the acquisition and manipulation of the time-resolved data. 9 figures, 4 tables.

Borst, W.L.

1984-01-01T23:59:59.000Z

287

Fluorescent Nanohybrids Based on Quantum DotChitosanAntibody as Potential Cancer Biomarkers  

Science Journals Connector (OSTI)

Photoluminescence (PL) characterization of the CdS/polysaccharides conjugates and intermediates systems was performed based on spectra acquired using the Nanodrop 3300 fluorospectrometer (Thermo Scientific, blue LED with ?excitation = 470 10 nm). ... All photoluminescence spectra were collected at room temperature, and measurements of fluorescence intensities were reported in relative fluorescent units (RFU). ... Additionally, QD colloidal media were placed inside a darkroom chamber where they were illuminated by a UV radiation emission bulb (?excitation = 254 and 365 nm, 6 W, Boitton Instruments). ...

Alexandra A. P. Mansur; Herman S. Mansur; Amanda Soriano-Arajo; Zlia I. P. Lobato

2014-06-23T23:59:59.000Z

288

Organic light-emitting device with a phosphor-sensitized fluorescent emission layer  

DOE Patents [OSTI]

The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters. The emissive region of the devices of the present invention comprise at least one phosphor-sensitized layer which has a combined emission from a phosphorescent emitter and a fluorescent emitter. In preferred embodiments, the invention relates to white-emitting OLEDS (WOLEDs).

Forrest, Stephen (Ann Arbor, MI); Kanno, Hiroshi (Osaka, JP)

2009-08-25T23:59:59.000Z

289

Flow method and apparatus for screening chemicals using micro x-ray fluorescence  

DOE Patents [OSTI]

Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow-separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.

Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Miller, Thomasin C. (Bartlesville, OK); Lewis, Cris (Los Alamos, NM); Mahan, Cynthia A. (Los Alamos, NM); Wells, Cyndi A. (Los Alamos, NM)

2009-04-14T23:59:59.000Z

290

On-line measurement of lignin in wood pulp by color shift of fluorescence  

DOE Patents [OSTI]

Lignin concentrations from wood pulp samples are measured by applying an excitation light at a selected wavelength to the samples in order to cause the lignin to emit fluorescence. A spectral distribution of the fluorescence emission is then determined. The lignin concentration is then calculated based on the spectral distribution signal. The spectral distribution is quantified by either a wavelength centroid method or a band ratio method. 6 figs.

Jeffers, L.A.; Malito, M.L.

1996-01-23T23:59:59.000Z

291

Stardust Interstellar Preliminary Examination VI: Quantitative elemental analysis by synchrotron X-ray fluorescence nanoimaging of eight impact features in aerogel  

E-Print Network [OSTI]

-ray fluorescence nanoimaging of eight impact features in aerogel Alexandre S. SIMIONOVICI1* , Laurence LEMELLE2

Nittler, Larry R.

292

N,N,NV,NV-tetramethyl-p-phenylenediamine initiates the appearance of a well-resolved I peak in the kinetics of chlorophyll fluorescence rise  

E-Print Network [OSTI]

fluorescence; Fm (=P)maximum level of chlorophyll fluorescence; Fv (=Fm?Fo), variable fluorescence; OEC, oxygen-evolving tissues, the initial fluorescence measured with all reaction centers in open state, Fo (O), rises to a peak level, Fm ( = P), observed when all reaction centers are closed, with two intermediate steps J (I1

Carpentier, Robert

293

Assessment of Nuclear Resonance Fluorescence for Spent Nuclear Fuel Assay  

SciTech Connect (OSTI)

In nuclear resonance fluorescence (NRF) measurements, resonances are excited by an external photon beam leading to the emission of gamma rays with specific energies that are characteristic of the emitting isotope. NRF promises the unique capability of directly quantifying a specific isotope without the need for unfolding the combined responses of several fissile isotopes as is required in other measurement techniques. We have analyzed the potential of NRF as a non-destructive analysis technique for quantitative measurements of Pu isotopes in spent nuclear fuel (SNF). Given the low concentrations of 239Pu in SNF and its small integrated NRF cross sections, the main challenge in achieving precise and accurate measurements lies in accruing sufficient counting statistics in a reasonable measurement time. Using analytical modeling, and simulations with the radiation transport code MCNPX that has been experimentally tested recently, the backscatter and transmission methods were quantitatively studied for differing photon sources and radiation detector types. Resonant photon count rates and measurement times were estimated for a range of photon source and detection parameters, which were used to determine photon source and gamma-ray detector requirements. The results indicate that systems based on a bremsstrahlung source and present detector technology are not practical for high-precision measurements of 239Pu in SNF. Measurements that achieve the desired uncertainties within hour-long measurements will either require stronger resonances, which may be expressed by other Pu isotopes, or require quasi-monoenergetic photon sources with intensities that are approximately two orders of magnitude higher than those currently being designed or proposed.This work is part of a larger effort sponsored by the Next Generation Safeguards Initiative to develop an integrated instrument, comprised of individual NDA techniques with complementary features, that is fully capable of determining Pu mass in spent fuel assemblies.

Quiter, Brian; Ludewigt, Bernhard; Ambers, Scott

2011-06-30T23:59:59.000Z

294

Hazard analysis of long term viewing of visible laser light off of fluorescent diffuse reflective surfaces (post-it).  

SciTech Connect (OSTI)

A laser hazard analysis is performed to evaluate if the use of fluorescent diffuse reflectors to view incident laser beams (Coherent Verdi 10W) present a hazard based on the ANSI Standard Z136.1-2000, American National Standard for the Safe Use of Lasers. The use of fluorescent diffuse reflectors in the alignment process does not pose an increased hazard because of the fluorescence at a different wavelength than that of the incident laser.

Augustoni, Arnold L.

2006-10-01T23:59:59.000Z

295

Fluorescence of PRODAN in Water: a Computational QM/MM MD Study  

SciTech Connect (OSTI)

Fluorescent properties of PRODAN (6-propionyl-2-dimethylaminonaphthalene) in water were studied by means of excited state molecular dynamics simulations employing a combined quantum mechanical and molecular mechanical approach with the time-dependent density functional theory (TD-DFT QM/MM MD). The state of the art coupled cluster method was used to benchmark density functional theory calculations. The influence of the water environment on PRODAN emission was investigated by employing several computational schemes with varying description of the solvent. The issue of the molecular geometry of the excited state PRODAN molecule in water was addressed. The experimental emission spectrum was reproduced for the planar excited state conformer of PRODAN in the extended environment of 300 explicit water molecules. The planar conformer was shown to be predominantly responsible for fluorescence. The twisted isomer is strongly stabilized in water, but rapidly evolve towards a conical intersection, and hence the twisted conformer is fluorescently inactive.

Pederzoli, Marek; Sobek, Lukas; Brabec, Jiri; Kowalski, Karol; Cwiklik, Lukasz; Pittner, Jiri

2014-03-28T23:59:59.000Z

296

Third Level Trigger for the Fluorescence Telescopes of the Pierre Auger Observatory  

E-Print Network [OSTI]

The trigger system for the Auger fluorescence telescopes is implemented in hard- and software for an efficient selection of fluorescence light tracks induced by high-energy extensive air showers. The algorithm of the third stage uses the multiplicity signal of the hardware for fast rejection of lightning events with above 99% efficiency. In a second step direct muon hits in the camera and random triggers are rejected by analyzing the space-time correlation of the pixels. The trigger algorithm was tested with measured and simulated showers and implemented in the electronics of the fluorescence telescopes. A comparison to a prototype trigger without multiplicity shows the superiority of this approach, e.g. the false rejection rate is a factor 10 lower.

A. Schmidt; T. Asch; H. Gemmeke; M. Kleifges; H. -J. Mathes; A. Menshikov; F. Schssler; D. Tcherniakhovski

2008-08-01T23:59:59.000Z

297

Third Level Trigger for the Fluorescence Telescopes of the Pierre Auger Observatory  

E-Print Network [OSTI]

The trigger system for the Auger fluorescence telescopes is implemented in hard- and software for an efficient selection of fluorescence light tracks induced by high-energy extensive air showers. The algorithm of the third stage uses the multiplicity signal of the hardware for fast rejection of lightning events with above 99% efficiency. In a second step direct muon hits in the camera and random triggers are rejected by analyzing the space-time correlation of the pixels. The trigger algorithm was tested with measured and simulated showers and implemented in the electronics of the fluorescence telescopes. A comparison to a prototype trigger without multiplicity shows the superiority of this approach, e.g. the false rejection rate is a factor 10 lower.

Schmidt, A; Gemmeke, H; Kleifges, M; Mathes, H -J; Menchikov, A; Schssler, F; Tcherniakhovski, D

2008-01-01T23:59:59.000Z

298

Fluorescence spectrum characteristic of ethanolwater excimer and mechanism of resonance energy transfer  

Science Journals Connector (OSTI)

The steady-state fluorescence spectrum characteristic of ethanolwater excimer has been studied in this paper. By analysing the features of the sharp emission spectrum with fine structures in a shortwave band and the characteristics of the broad and featureless fluorescence peaks in the longwave band, one can conclude that the excimers are formed between the new ethanolwater cluster molecules in the excited state and the ground state through the interaction among different chromophores. The excitation spectra in the two fluorescence bands have been studied, and their emission mechanisms have been ascertained based on the energy transfer theory. Furthermore, the critical distance of the resonance energy transfer has been calculated.

Liu Ying; Song Chun-Yuan; Luo Xiao-Sen; Lu Jian; Ni Xiao-Wu

2007-01-01T23:59:59.000Z

299

A portable time-domain LED fluorimeter for nanosecond fluorescence lifetime measurements  

SciTech Connect (OSTI)

Fluorescence lifetime measurements are becoming increasingly important in chemical and biological research. Time-domain lifetime measurements offer fluorescence multiplexing and improved handling of interferers compared with the frequency-domain technique. In this paper, an all solid-state, filterless, and highly portable light-emitting-diode based time-domain fluorimeter (LED TDF) is reported for the measurement of nanosecond fluorescence lifetimes. LED based excitation provides more wavelengths options compared to laser diode based excitation, but the excitation is less effective due to the uncollimated beam, less optical power, and longer latency in state transition. Pulse triggering and pre-bias techniques were implemented in our LED TDF to improve the peak optical power to over 100 mW. The proposed pulsing circuit achieved an excitation light fall time of less than 2 ns. Electrical resetting technique realized a time-gated photo-detector to remove the interference of the excitation light with fluorescence. These techniques allow the LED fluorimeter to accurately measure the fluorescence lifetime of fluorescein down to concentration of 0.5 ?M. In addition, all filters required in traditional instruments are eliminated for the non-attenuated excitation/emission light power. These achievements make the reported device attractive to biochemical laboratories seeking for highly portable lifetime detection devices for developing sensors based on fluorescence lifetime changes. The device was initially validated by measuring the lifetimes of three commercial fluorophores and comparing them with reported lifetime data. It was subsequently used to characterize a ZnSe quantum dot based DNA sensor.

Wang, Hongtao; Salthouse, Christopher D., E-mail: salthouse@ecs.umass.edu [Electrical and Computer Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Qi, Ying; Mountziaris, T. J. [Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States) [Center for Personalized Health Monitoring, University of Massachusetts, Amherst, Massachusetts 01003 (United States); Chemical Engineering Department, University of Massachusetts, Amherst, Massachusetts 01003 (United States)

2014-05-15T23:59:59.000Z

300

Application of synchrotron radiation to x-ray fluorescence analysis of trace elements  

SciTech Connect (OSTI)

The development of synchrotron radiation x-ray sources has provided the means to greatly extend the capabilities of x-ray fluorescence analysis for determinations of trace element concentrations. A brief description of synchrotron radiation properties provides a background for a discussion of the improved detection limits compared to existing x-ray fluorescence techniques. Calculated detection limits for x-ray microprobes with micrometer spatial resolutions are described and compared with experimental results beginning to appear from a number of laboratories. The current activities and future plans for a dedicated x-ray microprobe beam line at the National Synchrotron Light Source (NSLS) of Brookhaven National Laboratory are presented.

Gordon, B.M.; Jones, K.W.; Hanson, A.L.

1986-08-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Reflectance and Fluorescence Confocal Microscope for Imaging of the Mouse Colon  

E-Print Network [OSTI]

and fluorescence microscope. 20 3.3 Image Formation To form a 2D image using a point-scanning confocal microscope, the beam must be scanned in two dimensions using scanning mirrors. The PMT records a voltage signal from each point which is assigned... and fluorescence microscope. 20 3.3 Image Formation To form a 2D image using a point-scanning confocal microscope, the beam must be scanned in two dimensions using scanning mirrors. The PMT records a voltage signal from each point which is assigned...

Saldua, Meagan Alyssa

2012-02-14T23:59:59.000Z

302

Fast repetition rate (FRR) fluorometer and method for measuring fluorescence and photosynthetic parameters  

DOE Patents [OSTI]

A fast repetition rate fluorometer device and method for measuring in vivo fluorescence of phytoplankton or higher plants chlorophyll and photosynthetic parameters of phytoplankton or higher plants is revealed. The phytoplankton or higher plants are illuminated with a series of fast repetition rate excitation flashes effective to bring about and measure resultant changes in fluorescence yield of their Photosystem II. The series of fast repetition rate excitation flashes has a predetermined energy per flash and a rate greater than 10,000 Hz. Also, disclosed is a flasher circuit for producing the series of fast repetition rate flashes. 14 figs.

Kolber, Z.; Falkowski, P.

1995-06-20T23:59:59.000Z

303

A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)  

Science Journals Connector (OSTI)

A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO) ... Nitric oxide was passed through an Ascarite column and a 6 ft coil containing silica gel at ?78 C to remove impurities and then collected and stored under nitrogen in a gas storage bulb. ... Treatment of these cells with 3 mM Angelis salt led to an increase in fluorescence in both the NIR channel and the green channel (Figure 6C,E,H). ...

Alexandra T. Wrobel; Timothy C. Johnstone; Alexandria Deliz Liang; Stephen J. Lippard; Pablo Rivera-Fuentes

2014-02-24T23:59:59.000Z

304

Energy Dispersive X-ray Fluorescence Analysis of Sulfur in Biomass  

Science Journals Connector (OSTI)

An energy dispersive X-ray fluorescence (ED-XRF) spectroscopy method was developed to analyze low ppm level sulfur (S) in biomass feedstocks and in subsequent residues from pretreatment reactions. ... Representative biomass feedstocks and pretreatment residues were analyzed for S. ... The goal of this project was to determine whether an energy dispersive X-ray fluorescence (ED-XRF) spectroscopy method is effective in conducting sulfur analysis of woody biomass feedstocks at an appropriately useful sensitivity, especially when used to effectively monitor the extent of sulfur removal after biomass pretreatment reactions. ...

J. Michael Robinson; Staci R. Barrett; Kevin Nhoy; Rajesh K. Pandey; Joseph Phillips; Oscar M. Ramirez; Richard I. Rodriguez

2009-03-06T23:59:59.000Z

305

X-RAY FLUORESCENCE ANALYSIS OF HANFORD LOW ACTIVITY WASTE SIMULANTS METHOD DEVELOPMENT  

SciTech Connect (OSTI)

The x-ray fluorescence laboratory (XRF) in the Analytical Development Directorate (ADD) of the Savannah River National Laboratory (SRNL) was requested to develop an x-ray fluorescence spectrometry method for elemental characterization of the Hanford Tank Waste Treatment and Immobilization Plant (WTP) pretreated low activity waste (LAW) stream to the LAW Vitrification Plant. The WTP is evaluating the potential for using XRF as a rapid turnaround technique to support LAW product compliance and glass former batching. The overall objective of this task was to develop an XRF analytical method that provides rapid turnaround time (<8 hours), while providing sufficient accuracy and precision to determine variations in waste.

Jurgensen, A; David Missimer, D; Ronny Rutherford, R

2007-08-08T23:59:59.000Z

306

Investigation and technique in the fluorescent spectra examination of crude oil  

E-Print Network [OSTI]

by chromato- graphy and distillation. The adsorbent was Alcoa activated alumina, chromatographic grade F-20. Support for the adsorbent was Pyrex brand glass fibre and cleaned B 0, A. sea sand. 11 DEVELOPl';, 'EI&T OF TEOHN IQUE Fluorescent intensity..., the cut between 2F2 and 2P$ was exactly between the two bands. The similarity of the fluorescent spectra of 1E1, 1E2, and 1E?i with 2F1, 2P2, and 2F$ (Figures 11, 12, 1B and 19) shows the similarity in the developing power of chloroform and. ether. A...

Chambers, Gilbert Vester

1958-01-01T23:59:59.000Z

307

$\\mathrm{CO_2}$ exploding clusters dynamics probed by XUV fluorescence  

E-Print Network [OSTI]

Clusters excited by intense laser pulses are a unique source of warm dense matter, that has been the subject of intensive experimental studies. The majority of those investigations concerns atomic clusters, whereas the evolution of molecular clusters excited by intense laser pulses is less explored. In this work we trace the dynamics of $\\mathrm{CO_2}$ clusters triggered by a few-cycle 1.45-$\\mu$m driving pulse through the detection of XUV fluorescence induced by a delayed 800-nm ignition pulse. Striking differences among fluorescence dynamics from different ionic species are observed.

Negro, M; Fabre, B; Dorchies, F; Devetta, M; Staedter, D; Vozzi, C; Mairesse, Y; Stagira, S

2014-01-01T23:59:59.000Z

308

Method for detecting binding events using micro-X-ray fluorescence spectrometry  

DOE Patents [OSTI]

Method for detecting binding events using micro-X-ray fluorescence spectrometry. Receptors are exposed to at least one potential binder and arrayed on a substrate support. Each member of the array is exposed to X-ray radiation. The magnitude of a detectable X-ray fluorescence signal for at least one element can be used to determine whether a binding event between a binder and a receptor has occurred, and can provide information related to the extent of binding between the binder and receptor.

Warner, Benjamin P. (Los Alamos, NM); Havrilla, George J. (Los Alamos, NM); Mann, Grace (Hong Kong, HK)

2010-12-28T23:59:59.000Z

309

First-Principles Characterization of the Energy Landscape and Optical Spectra of Green Fluorescent Protein along the AIB  

E-Print Network [OSTI]

First-Principles Characterization of the Energy Landscape and Optical Spectra of Green Fluorescent Supporting Information ABSTRACT: Structures and optical spectra of the green fluorescent protein (GFP) forms-form) is lowest in energy, whereas the systems with the anionic chromophore (B- and I-forms) are about 1 kcal

Krylov, Anna I.

310

Reversible Modification of CdSe-CdS/ZnS Quantum Dot Fluorescence by Surrounding Ca2+  

E-Print Network [OSTI]

Reversible Modification of CdSe-CdS/ZnS Quantum Dot Fluorescence by Surrounding Ca2+ Ions Li Li (3-MPA) coated CdSe-CdS/ZnS core-multishell QDs when free Ca2+ ions were added to and subsequently removed from the QD solution. It was found that QD fluorescence intensity was reduced when Ca2+ ions were

Haviland, David

311

Global analysis of Fo rster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting  

E-Print Network [OSTI]

Global analysis of Fo¨ rster resonance energy transfer in live cells measured by fluorescence of Fo¨ rster resonance energy transfer (FRET) in live cells using the rise time of acceptor fluorescence those molecules that are involved in the energy-transfer process are monitored. This contrasts

van Stokkum, Ivo

312

Single-Molecule Fluorescence Studies of Protein Folding and Conformational Xavier Michalet,* Shimon Weiss, and Marcus Jager*  

E-Print Network [OSTI]

Single-Molecule Fluorescence Studies of Protein Folding and Conformational Dynamics Xavier Michalet. Single-Molecule Fluorescence Studies of Protein Folding and Conformations at Equilibrium 1796 4-Molecule Protein Folding under Nonequilibrium Conditions 1808 6. Conclusion 1809 7. Acknowledgments 1810 8

Michalet, Xavier

313

Simple Method to Enhance the Photostability of the Fluorescence Reporter R6G for Prolonged Single-Molecule Studies  

E-Print Network [OSTI]

Simple Method to Enhance the Photostability of the Fluorescence Reporter R6G for Prolonged Single-Molecule, Philadelphia, Pennsylvania 19104, United States ABSTRACT: For fluorescence-based single-molecule studies, photo for single-molecule detection but can also significantly increase the survival time of individual R6G

Shorter, James

314

Photophysics of DsRed, a Red Fluorescent Protein, from the Ensemble to the Single-Molecule Level  

E-Print Network [OSTI]

Photophysics of DsRed, a Red Fluorescent Protein, from the Ensemble to the Single-Molecule Level B-wavelength substitute for green fluorescent protein (GFP) mutants for in vivo protein labeling. Bulk and single-molecule is not an issue in physiological studies. Introduction Single-molecule spectroscopy (SMS) continues to play

Boxer, Steven G.

315

Excitation of sensitized fluorescence of europium and curium in an aqueous solution of thenoyltrifluoroacetone by a nitrogen laser  

SciTech Connect (OSTI)

The fluorescence spectrum of trivalent europium in aqueous solutions of thenoyltrifluoroacetone, excited by a nitrogen laser with emission wavelength 337 nm, exhibits bands at 582, 593, 616, 650, and 695 nm. Two bands appear in the fluorescence spectrum of trivalent curium under the same conditions - at 598 and 607 nm. The times of quenching of the fluorescence of the ions of these elements were measured, both in H/sub 2/O medium and in D/sub 2/O. A linear relationship was found between the fluorescence intensity of europium and curium and their concentration in TTA solution. The limit of determination of europium and curium by the fluorescent method with laser excitation using the bands at 615 and 607 nm proved equal to 0.3 and 0.07 ng/ml, respectively.

Dem'yanova, T.A.; Stepanov, A.V.; Babaev, A.S.; Aleksandruk, V.M.

1987-03-01T23:59:59.000Z

316

Novel Al2O3:C,Mg fluorescent nuclear track detectors for passive neutron dosimetry  

Science Journals Connector (OSTI)

......technique based on laser scanning and...do not require protection from visible...elsewhere(5). Laser-induced fluorescence...635-nm red laser diode without photoionisation...piezoelectric actuator to detect the...technique based on laser scanning and...methods Radiation Protection instrumentation......

G. Jeff Sykora; Mark S. Akselrod; M. Salasky; Stephen A. Marino

2007-08-01T23:59:59.000Z

317

Laser-excited atomic fluorescence spectrometry in a graphite furnace with an  

E-Print Network [OSTI]

Laser-excited atomic fluorescence spectrometry in a graphite furnace with an optical parametric oscillator laser for sequential multi-element determination of cadmium, cobalt, lead, manganese and thallium, for the ® rst time, that solid-state lasers required for analysis (ml or mg) and the technique has direct based

Michel, Robert G.

318

Micro-Raman Spectroscopy of Algae: Composition Analysis and Fluorescence Background Behavior  

E-Print Network [OSTI]

analysis; biodiesel Introduction To date, analysis of algae composition is a multi-staged processARTICLE Micro-Raman Spectroscopy of Algae: Composition Analysis and Fluorescence Background performed using Stokes Raman scattering for compositional analysis of algae. Two algal species, Chlorella

319

An efficient fluorescent sensing platform for biomolecules based on fenton reaction triggered molecular beacon cleavage strategy  

E-Print Network [OSTI]

An efficient fluorescent sensing platform for biomolecules based on fenton reaction triggered/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha: Molecular beacons Fenton reaction Glucose Choline Biosensor a b s t r a c t A universal sensing platform

Tan, Weihong

320

Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and  

E-Print Network [OSTI]

Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot Jackson¶ Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring between epidermal cells of Arabidopsis and onion. When expressed in vivo, the GFP KN1 fusion trafficked

Jackson, David

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms  

DOE Patents [OSTI]

In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.

Seibert, Michael (Lakewood, CO); Makarova, Valeriya (Golden, CO); Tsygankov, Anatoly A. (Pushchino, RU); Rubin, Andrew B. (Moscow, RU)

2007-06-12T23:59:59.000Z

322

Fluorescent probes for non-invasive bioenergetic studies of whole cyanobacterial cells  

E-Print Network [OSTI]

Fluorescent probes for non-invasive bioenergetic studies of whole cyanobacterial cells Markus of bioenergetic processes in whole cells of the cyanobacterium Synechocystis sp. PCC 6803. Acridine yellow to be created. In sum- mary, bioenergetic £uorescence measurement com- bines the advantages of an easy

Roegner, Matthias

323

Effect of Combining Far-Red Light with Shorter Wave Light on the Excitation of Fluorescence  

E-Print Network [OSTI]

of Fluorescence in Chlorella Recent experiments on the rate of photosyn- thesis in far-red light alone that the quantum yield of Auo- rescence in Chlorella drops when excitation is achieved by light above 680 ml* (10- teen experiments of this type are summarized in Table I. Chlorella pyrenoidosa Strain 3 were used

Govindjee

324

Proceedings of ICRC 2001: 741 c Copernicus Gesellschaft 2001 Calibration of the Auger Fluorescence Telescopes  

E-Print Network [OSTI]

Norte 304, (5613) Malarg¨ue, Argentina Abstract. Thirty fluorescence telescopes in four stations development (Xmax) and determi- nation of the absolute energy of EHE events. A telescope camera contains 440 in Argentina are discussed. 1 Introduction The southern hemisphere experiment of the Pierre Auger Ob- servatory

325

28th International Cosmic Ray Conference 465 Atmospheric Monitoring for the Pierre Auger Fluorescence  

E-Print Network [OSTI]

, Albuquerque, NM 87131 (2) Observatorio Pierre Auger, Malarg¨ue, (5613) Mendoza, Argentina Abstract Major uncertainties in the fluorescence energy measurement come from the precision of various atmospheric transmission, is summarized in this paper. 1. Introduction A reliable air shower energy scale and energy resolution is central

326

Video-Rate Scanning Two-Photon Excitation Fluorescence Microscopy and Ratio Imaging with Cameleons  

E-Print Network [OSTI]

Video-Rate Scanning Two-Photon Excitation Fluorescence Microscopy and Ratio Imaging with Cameleons ABSTRACT A video-rate (30 frames/s) scanning two-photon excitation microscope has been successfully tested 690 to 1050 nm, prechirper optics for laser pulse-width compression, resonant galvanometer for video

Tsien, Roger Y.

327

Fluorescent silica colloids for study and visualization of skin care products  

E-Print Network [OSTI]

due to long exposures to cold and dry air (7). Different skin care products are used to hy- drate dryFluorescent silica colloids for study and visualization of skin care products Swaminathan Iyer: The efficacy of skin care products depends on the time and dynamics of their absorbance by the skin, and its

Sokolov, Igor

328

Deep-Sea Research I 50 (2003) 417434 Phytoplankton natural fluorescence variability in the  

E-Print Network [OSTI]

capacity and community composi- tion. Solar-stimulated, or ``natural'' fluorescence is a passive of California, Santa Barbara, Santa Barbara, CA 93106-3060, USA Received 10 October 2001; received in revised Science, University of California, Santa Barbara, Santa Barbara, CA 93106-3060, USA. Fax: +1

California at Santa Barbara, University of

329

Preliminary evaluation of a fluorescence and radioisotope nuclear smuggling deterrence tag - final report (IL500E)  

SciTech Connect (OSTI)

This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials, (2) inventory control of long-term stored items containing special nuclear materials, and (3) tracking materials transferred between facilities. The system uses three types of materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. This report also summarizes the efforts completed in identifying hardware that will be used for the tagging system. This hardware includes the devices for applying the tagging materials, the commercially available fluorescence detection systems, and gamma ray detection systems assembled from existing, commercially available technologies.

Hartenstein, S.D.; Aryaeinejad, R.; Delmastro, J.R. [and others

1997-04-01T23:59:59.000Z

330

Hybrid Electrochromic Fluorescent Poly(DNTD)/CdSe@ZnS Composite Films  

E-Print Network [OSTI]

Hybrid Electrochromic Fluorescent Poly(DNTD)/CdSe@ZnS Composite Films Huige Wei, Xingru Yan, Springdale, Arkansas 72764, United States ABSTRACT: Hybrid electrochromic poly(DNTD)/CdSe@ZnS quantum dots of an electrical current after the application of an appropriate electrode potential.15-17 The electrochromic

Guo, John Zhanhu

331

Abstract--A low noise optical sensor and biocompatible microscale optical filters for integrated fluorescence sensors  

E-Print Network [OSTI]

Abstract--A low noise optical sensor and biocompatible microscale optical filters for integrated fluorescence sensors were developed and tested. The sensor was fabricated in a 0.5 µm CMOS process. The measured reset noise of the sensor is reduced by a factor of 10 compared to conventional active pixel

Maryland at College Park, University of

332

Strategies for laser-induced fluorescence detection of nitric oxide in high-pressure flames.  

E-Print Network [OSTI]

engines and gas turbines where the combustion chamber is at high pressure. Despite the use of catalyticStrategies for laser-induced fluorescence detection of nitric oxide in high-pressure flames. II. A combustion systems3�13 and thus develop new schemes to mini- mize NO effluent. The high operating pressure

Lee, Tonghun

333

Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens.  

Science Journals Connector (OSTI)

...problems with the commercialization of these beneficial...characteristics for this process and the performance...summarized. The commercialization of fluorescent...facilitate this process by enabling the...problems with the commercialization of these beneficial...characteristics for this process and the performance...

D J O'Sullivan; F O'Gara

1992-12-01T23:59:59.000Z

334

Low-energy X-ray fluorescence microscopy opening new opportunities for bio-related research  

Science Journals Connector (OSTI)

...and (g) zinc. Photon energy: 1467 eV. Dwell time...magnesium. Excitation photon energy 1686 eV. Dwell time per...Development of a low-energy X-ray fluorescence system...Spence), pp. 835-926. New York, NY: Springer. ( doi...

2009-01-01T23:59:59.000Z

335

Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies  

E-Print Network [OSTI]

Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies for protein folding studies and has been extensively stud- ied, both experimentally (at the ensemble level concentration. It is shown that new infor- mation about different aspects of the protein folding reaction can

Croquette, Vincent

336

Fluorescent chemosensor for reactive organohalides in micellar solution with an example of autocatalysiswz  

E-Print Network [OSTI]

-alkylation reaction by forming a non-covalent pre-reaction complex that undergoes a rapid SN2 reaction inside-Alkylation of a fluorescent macrocyclic amine in aqueous micellar solution produces enhanced emission; the reaction The sensing effect is due to an accelerated N-alkylation reaction that produces a quaternary ammonium product

Smith, Bradley D.

337

Single-molecule fluorescence studies from a bioinorganic perspective Peng Chen *, Nesha May Andoy  

E-Print Network [OSTI]

Review Single-molecule fluorescence studies from a bioinorganic perspective Peng Chen *, Nesha May. Solomon. Abstract In recent years, single-molecule methods have enabled many innovative studies machineries. Single-molecule studies of bioinorganic systems have been limited, how- ever, even though

Chen, Peng

338

Whats in an EEM? Molecular Signatures Associated with Dissolved Organic Fluorescence in Boreal Canada  

Science Journals Connector (OSTI)

Molecular Signatures Associated with Dissolved Organic Fluorescence in Boreal Canada ... Of these three humic-like components, P3 was the most photolabile,(19) possibly because its excitation maximum extends furthest into the solar spectrum(30) (Table 1; SI Figure S1). ...

A. Stubbins; J.-F. Lapierre; M. Berggren; Y. T. Prairie; T. Dittmar; P. A. del Giorgio

2014-08-22T23:59:59.000Z

339

Fluorescence-Based Quasicontinuous and In Situ Monitoring of Biofilm Formation Dynamics in Natural Marine Environments  

Science Journals Connector (OSTI)

...and about 90% from the first 10 mm), deep enough...exposed to an optical output power of about 600 muW, and...measurement cycles. The first measurement, which was...compositions of lakes and oceans: a first comparison based on fluorescence...

M. Fischer; G. Friedrichs; T. Lachnit

2014-04-11T23:59:59.000Z

340

Can we measure terrestrial photosynthesis from space directly, using spectral reflectance and fluorescence?  

E-Print Network [OSTI]

Can we measure terrestrial photosynthesis from space directly, using spectral reflectance the changes in the xanthophyll cycle, which is closely coupled to photosynthesis. Several studies have shown of fluorescence, which is a directly related to the efficiency of photosynthesis. We discuss the state of the art

Jones, Peter JS

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Dynamics of Vacuoles and H+-Pyrophosphatase Visualized by Monomeric Green Fluorescent Protein in Arabidopsis: Artifactual Bulbs and Native Intravacuolar Spherical Structures  

Science Journals Connector (OSTI)

...an artifactual fluorescence resonance energy transfer reaction on membranes (Zacharias...reported. In a fluorescence resonance energy transfer assay for membrane proteins...1507-1523. Espiau, B. , Lemercier, G., Ambit, A., Bringaud, F., Merlin, G...

Shoji Segami; Sachi Makino; Ai Miyake; Mariko Asaoka; Masayoshi Maeshima

2014-08-12T23:59:59.000Z

342

Frequency measurements of saturated-fluorescence-stabilized CO2 laser lines: comparison with an OsO4-stabilized CO2 laser standard  

Science Journals Connector (OSTI)

frequency grid, to test the performance of the saturated fluorescence method for CO2 laser frequency stabilization. The Allan variance for the saturated-fluorescence-stabilized CO2 laser reached 60Hz for an int...

B. Frech; L.F. Constantin; A. Amy-Klein; O. Phavorin; C. Daussy

1998-08-01T23:59:59.000Z

343

Synthesis of antibodies-conjugated fluorescent dye-doped silica nanoparticles for a rapid single step detection of campylobacter jejuni in live poultry  

Science Journals Connector (OSTI)

The preparation of antibodies-conjugated fluorescent dye-doped silica nanoparticles (FDS-NPs) was developed to detect Campylobacter jejuni cells under a fluorescence microscope. The particles prepared by sol-gel microemulsion techniques have a round ...

Wachira Tansub; Kooranee Tuitemwong; Pichet Limsuwan; Supannee Theparoonrat; Pravate Tuitemwong

2012-01-01T23:59:59.000Z

344

(Funding source: NASA Grant NNC04GB52G, 2004-2009 and NSF Grant CTS-0086988, 2001-2005) Invention Summary For: "Fluorescence and Fiber-Optics  

E-Print Network [OSTI]

and temperature control (with the help of thermo-electric coolers) of the laser housing, fluorescence meter

Narain, Amitabh

345

Green Fluorescent Protein-Labeled Monitoring Tool To Quantify Conjugative Plasmid Transfer between Gram-Positive and Gram-Negative Bacteria  

Science Journals Connector (OSTI)

...February 2012 research-article Methods Green Fluorescent Protein-Labeled Monitoring...Germany. On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring...and Espinosa (30) have constructed a green fluorescent protein (GFP)-tagged derivative...

Karsten Arends; Katarzyna Schiwon; Trkan Sakinc; Johannes Hbner; Elisabeth Grohmann

2011-12-02T23:59:59.000Z

346

Air Fluorescence Calorimetry with the High Resolution Fly's Eye and Telescope Array Experiments  

SciTech Connect (OSTI)

The air fluorescence technique was first successfully deployed on the Fly's Eye Experiment (1981-1993) by the University of Utah. Its successor, the High Resolution Fly's Eye (HiRes) experiment has further exploited this technique, first in hybrid mode with the MIA muon array (1993-1996), and then in monocular and stereoscopic modes (1997-2006). Results from HiRes will be presented, including evidence for the Greisen-Zatsepin-K'uzmin (GZK) Effect predicted 40 years ago. Most recently, members of the HiRes are collaborating with groups from Japan, led by University of Tokyo, to construct and operate the Telescope Array (TA) experiment, which will deploy a large scintillation-based ground array in combination with fluorescence detectors. Funding for TA in the US has already been approved by NSF. TA will begin operation in 2007.

Jui, Charles C. H. [Department of Physics, University of Utah Salt Lake City, Utah (United States)

2006-10-27T23:59:59.000Z

347

Artificial neural networks for processing fluorescence spectroscopy data in skin cancer diagnostics  

Science Journals Connector (OSTI)

Over the years various optical spectroscopic techniques have been widely used as diagnostic tools in the discrimination of many types of malignant diseases. Recently, synchronous fluorescent spectroscopy (SFS) coupled with chemometrics has been applied in cancer diagnostics. The SFS method involves simultaneous scanning of both emission and excitation wavelengths while keeping the interval of wavelengths (constant-wavelength mode) or frequencies (constant-energy mode) between them constant. This method is fast, relatively inexpensive, sensitive and non-invasive. Total synchronous fluorescence spectra of normal skin, nevus and melanoma samples were used as input for training of artificial neural networks. Two different types of artificial neural networks were trained, the self-organizing map and the feed-forward neural network. Histopathology results of investigated skin samples were used as the gold standard for network output. Based on the obtained classification success rate of neural networks, we concluded that both networks provided high sensitivity with classification errors between 2 and 4%.

L Lenhardt; I Zekovi?; T Drami?anin; M D Drami?anin

2013-01-01T23:59:59.000Z

348

Approximate Marginalization of Absorption and Scattering in Fluorescence Diffuse Optical Tomography  

E-Print Network [OSTI]

In fluorescence diffuse optical tomography (fDOT), the reconstruction of the fluorophore concentration inside the target body is usually carried out using a normalized Born approximation model where the measured fluorescent emission data is scaled by measured excitation data. One of the benefits of the model is that it can tolerate inaccuracy in the absorption and scattering distributions that are used in the construction of the forward model to some extent. In this paper, we employ the recently proposed Bayesian approximation error approach to fDOT for compensating for the modeling errors caused by the inaccurately known optical properties of the target in combination with the normalized Born approximation model. The approach is evaluated using a simulated test case with different amount of error in the optical properties. The results show that the Bayesian approximation error approach improves the tolerance of fDOT imaging against modeling errors caused by inaccurately known absorption and scattering of the...

Mozumder, Meghdoot; Arridge, Simon; Kaipio, Jari P; d'Andrea, Cosimo; Kolehmainen, Ville

2015-01-01T23:59:59.000Z

349

Doppler-shifted fluorescence imaging of velocity fields in supersonic reacting flows  

SciTech Connect (OSTI)

The application of Doppler-shifted fluorescence imaging of velocity fields in supersonic reacting flows is analyzed. Focussing on fluorescence of the OH molecule in typical H2-air Scramjet flows, the effects of uncharacterized variations in temperature, pressure, and collisional partner composition across the measurement plane are examined. Detailed measurements of the (1,0) band OH lineshape variations in H2-air combustions are used, along with single-pulse and time-averaged measurements of an excimer-pumped dye laser, to predict the performance of a model velocimeter with typical Scramjet flow properties. The analysis demonstrates the need for modification and control of the laser bandshape in order to permit accurate velocity measurements in the presence of multivariant flow properties. 13 refs.

Allen, M.G.; Davis, S.J.; Kessler, W.J.; Sonnenfroh, D.M. (Physical Sciences, Inc., Andover, MA (United States))

1992-07-01T23:59:59.000Z

350

Interplay between excited-state intramolecular proton transfer and charge transfer in flavonols and their use as protein-binding-site fluorescence probes  

SciTech Connect (OSTI)

A comparative study is presented of competitive fluorescences of three flavonols, 3-hydroxyflavone, 3,3[prime],4[prime],7-tetrahydroxyflavone (fisetin), and 4[prime]-diethylamino-3-hydroxyflavone (DHF). The normal fluorescence S[sub 1] [yields] S[sub 0] (400-nm region) is largely replaced by the proton-transfer tautomer fluorescence S[prime][sub 1] [yields] S[prime][sub 0] in the 550-nm region for all three of the flavonols in aprotic solvents at room temperature. For DHF in polar solvents the normal fluorescence becomes a charge-transfer fluorescence (460-500 nm) which competes strongly with the still dominant proton-transfer fluorescence (at 570 nm). In protic solvents, and at 77 K, the interference with intramolecular hydrogen bonding gives rise to greatly enhanced normal fluorescence, lowering the quantum yield of proton-transfer fluorescence. The utility of DHF as a discriminating fluorescence probe for protein binding sites is suggested by the strong dependence of the charge-transfer fluorescence on polarity of the environment and by various static and dynamic parameters of the charge-transfer and proton-transfer fluorescence which can be determined. 49 refs., 6 figs., 1 tab.

Sytnik, A.; Gormin, D.; Kasha, M. (Florida State Univ., Tallahassee, FL (United States))

1994-12-06T23:59:59.000Z

351

Improved fluorescence-enhanced optical imaging and tomography by enhanced excitation light rejection  

E-Print Network [OSTI]

) .................................................................88 xvi NOMENCLATURE Abbreviations 2-D Two-dimension 3-D Three-dimension ACR Amplitude ratio AOI Angle of incidence CCD Charge coupled device CT Computed tomography CW... molecular markers of disease at pico-molar to nano-molar concentrations. Light in the UV-visible range does not enable deep 7 penetration for fluorescence imaging mainly because of autofluorescence and a high level of endogenous absorbers...

Hwang, Kil Dong

2009-05-15T23:59:59.000Z

352

MESSENGER detection of electron-induced X-ray fluorescence from Mercury's surface  

E-Print Network [OSTI]

. Rhodes,4 Charles E. Schlemm II,4 Sean C. Solomon,3,5 and Pavel M. Trávnícek6 Received 2 May 2012; revised methodologies. Derived S and Ca abundances are somewhat higher than derived from the solar-induced fluorescence. Schlemm II, S. C. Solomon, and P. M. Trávnícek (2012), MESSENGER detection of electron-induced X

California at Berkeley, University of

353

Growth and pigment content of Gracilaria tikvahiae McLachlan under fluorescent and LED lighting  

Science Journals Connector (OSTI)

Abstract Light emitting diode (LED) technology has significant potential advantages over other light sources in algal aquaculture. This study investigated \\{LEDs\\} as light sources for the culture of Gracilaria tikvahiae. We cultured a wild-type and a green mutant strain of G. tikvahiae, comparing growth rate and tissue chlorophyll a, total carotenoids, and phycobiliprotein concentrations under high output cool white fluorescent, pure primary color LED, and mixed LED lighting. Under monochromatic light, the growth rates under high output cool white fluorescent lighting were significantly higher than rates under pure LED light (all three colors for wild strain and green and blue for green mutant). However, when pure color LED lighting was mixed (50%/50%), the red+green (wild-type strain and green mutant) and the green+blue LED combinations (wild-type only) showed growth rates similar to those under high output cool white fluorescent lighting. In the trichromatic experiment, growth of the wild-type strain under mixed three-color (40%/40%/20%) LED light was indistinguishable from those of the fluorescent control lighting. Chlorophyll a and carotenoid concentrations of Gracilaria grown in the dichromatic light experiment were 55% and 74% higher, respectively, under red+blue LED lighting than under the other light treatments. The wild-type strain of G. tikvahiae possessed significantly greater concentrations of chlorophyll a, and phycoerythrin than did the green mutant, while green mutant thalli had higher phycocyanin levels. With rising LED efficiency and energy savings, \\{LEDs\\} will be an increasingly better choice for indoor seaweed cultivation, especially if control of pigment production and morphogenesis by selective use of particular wavelengths is desirable.

Jang K. Kim; Yunxiang Mao; George Kraemer; Charles Yarish

2015-01-01T23:59:59.000Z

354

Electromagnetically induced transparency and dark fluorescence in a cascade three-level diatomic lithium system  

Science Journals Connector (OSTI)

Following our previous brief report [Phys. Rev. Lett. 88, 173003 (2002)], we report here a detailed study of electromagnetically induced transparency (EIT) and dark fluorescence in a cascade three-level diatomic lithium system using optical-optical double resonance (OODR) spectroscopy for both resonance and off resonance coupling. When a strong coupling laser couples the intermediate state A?u+1(v=13,J=14) to the upper state G?g1(v=11,J=14) of Li27, the fluorescence from both A?u+1 and G?g1 states was drastically reduced as the weak probe laser was tuned through the resonance transition between the ground state X?g+1(v=4,J=15) and the excited state A?u+1(v=13,J=14). The strong coupling laser makes an optically thick medium transparent for the probe transition. In addition, the fact that fluorescence from the upper state G?g1(v=11,J=14) was also dark when both lasers were tuned at resonance implies that the molecules were trapped in the ground state. We used density matrix methods to simulate the response of an open molecular three-level system to the action of a strong coupling field and a weak probe field. The analytical solutions were obtained under the steady-state condition. We have incorporated the magnetic sublevel (M) degeneracy of the rotational levels in the line shape analysis and report ?M? dependent line shape splitting. Our theoretical calculations are in excellent agreement with the observed fluorescence spectra. We show that the coherence is remarkably preserved even when the coupling field was detuned far from the resonance.

Jianbing Qi and A. Marjatta Lyyra

2006-04-19T23:59:59.000Z

355

Quantitative Modeling and Estimation in Systems Biology using Fluorescent Reporter Systems  

E-Print Network [OSTI]

) Fluorescence intensity profile obtained for IL-6-STAT3 system ............. 45 9. Estimated STAT3 profiles from Tikhonov regularization and truncated SVD ........ 47 10. Illustration for choosing the excitation wavelength for a mixture containing CFP and GFP... (data obtained from an online database (Biosciences, 2000) for CFP and GFP variants that have maximum emission and excitation wavelengths reasonably close to pAmCyan and pCM18 protein plasmids, respectively...

Bansal, Loveleena

2013-12-10T23:59:59.000Z

356

2015-01-28 Issuance: Test Procedure for Fluorescent Lamp Ballasts; Final Rule Correction  

Broader source: Energy.gov [DOE]

This document is a pre-publication Federal Register final rule correction regarding test procedures for fluorescent lamp ballasts, as issued by the Deputy Assistant Secretary for Energy Efficiency on January 28, 2014. Though it is not intended or expected, should any discrepancy occur between the document posted here and the document published in the Federal Register, the Federal Register publication controls. This document is being made available through the Internet solely as a means to facilitate the public's access to this document.

357

Through Skull Fluorescence Imaging of the Brain in a New Near-Infrared Window  

E-Print Network [OSTI]

To date, brain imaging has largely relied on X-ray computed tomography and magnetic resonance angiography with limited spatial resolution and long scanning times. Fluorescence-based brain imaging in the visible and traditional near-infrared regions (400-900 nm) is an alternative but currently requires craniotomy, cranial windows and skull thinning techniques, and the penetration depth is limited to 1-2 mm due to light scattering. Here, we report through-scalp and through-skull fluorescence imaging of mouse cerebral vasculature without craniotomy utilizing the intrinsic photoluminescence of single-walled carbon nanotubes in the 1.3-1.4 micrometre near-infrared window. Reduced photon scattering in this spectral region allows fluorescence imaging reaching a depth of >2 mm in mouse brain with sub-10 micrometre resolution. An imaging rate of ~5.3 frames/s allows for dynamic recording of blood perfusion in the cerebral vessels with sufficient temporal resolution, providing real-time assessment of blood flow anomaly...

Hong, Guosong; Chang, Junlei; Antaris, Alexander L; Chen, Changxin; Zhang, Bo; Zhao, Su; Atochin, Dmitriy N; Huang, Paul L; Andreasson, Katrin I; Kuo, Calvin J; Dai, Hongjie

2014-01-01T23:59:59.000Z

358

Spatial assessment of net mercury emissions from the use of fluorescent bulbs  

SciTech Connect (OSTI)

While fluorescent lighting is an important technology for reducing electrical energy demand, mercury used in the bulbs is an ongoing concern. Using state and country level data, net emissions of mercury from the marginal use of fluorescent lightbulbs are examined for a base year of 2004 for each of the 50 United States and 130 countries. Combustion of coal for electric power generation is generally the largest source of atmospheric mercury pollution; reduction in electricity demand from the substitution of incandescent bulbs with fluorescents leads to reduced mercury emissions during the use of the bulb. This analysis considers the local mix of power sources, coal quality, thermal conversion efficiencies, distribution losses, and any mercury control technologies that might be in place. Emissions of mercury from production and end-of-life treatment of the bulbs are also considered, providing a life-cycle perspective. Net reductions in mercury over the entire life cycle range from -1.2 to 97 mg per bulb depending on the country. The consequences for atmospheric mercury emissions of several policy scenarios are also discussed. 46 refs., 4 figs., 3 tabs.

Matthew J. Eckelman; Paul T. Anastas; Julie B. Zimmerman [Yale University, New Haven, CT (United States). Department of Chemical Engineering

2008-11-15T23:59:59.000Z

359

H_2 Absorption and Fluorescence for Gamma Ray Bursts in Molecular Clouds  

E-Print Network [OSTI]

If a gamma ray burst with strong UV emission occurs in a molecular cloud, there will be observable consequences resulting from excitation of the surrounding H2. The UV pulse from the GRB will pump H2 into vibrationally-excited levels which produce strong absorption at wavelengths < 1650 A. As a result, both the prompt flash and later afterglow will exhibit strong absorption shortward of 1650 A, with specific spectroscopic features. Such a cutoff in the emission from GRB 980329 may already have been observed by Fruchter et al.; if so, GRB 980329 was at redshift 3.0 < z < 4.4 . BVRI photometry of GRB 990510 could also be explained by H2 absorption if GRB 990510 is at redshift 1.6 < z < 2.3. The fluorescence accompanying the UV pumping of the H2 will result in UV emission from the GRB which can extend over days or months, depending on parameters of the ambient medium and beaming of the GRB flash. The 7.5-13.6 eV fluorescent luminosity is \\sim 10^{41.7} erg/s for standard estimates of the parameters of the GRB and the ambient medium. Spectroscopy can distinguish this fluorescent emission from other possible sources of transient optical emission, such as a supernova.

B. T. Draine

1999-07-16T23:59:59.000Z

360

A comparative study on the stability and structure of two different green fluorescent proteins in organic co-solvent systems  

Science Journals Connector (OSTI)

Green fluorescent protein (GFP) has been used ... requires the stability of GFP in organic co-solvent systems. This prompted us to examine the ... stability, under a range of organic co-solvent systems. n-GFP and...

Govindan Raghunathan; Sriram Sokalingam

2013-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Analysis of Na and Ba in an atomic fluorescence method with excitation by a pulsed dye laser with lamp pumping  

SciTech Connect (OSTI)

Among the laser methods used in atomic spectroscopy, atomic fluorescence resonance spectroscopy together with an atomic ionization technique make it possible to work with individual atoms. This paper reports on the determination of Na and Ba in aqueous solutions of their salts with atomic fluorescence analysis performed wih a dye laser and lamp pumping, the pulse of generation having a length of 1-2 usec. The detection limit of elements in atomic fluorescence analysis performed wih dye lasers with lamp pumping can be reduced by increasing the spectral density of the laser radiation through narrowing the line of generation, by employing other dyes to excite lines of greater intensity, by improving the recording system of the fluorescence spectra, and by improving the atomization of the samples to be analyzed.

Kukvshim, V.G.

1986-04-01T23:59:59.000Z

362

Polyhalogenated hydrocarbon refrigerants and refrigerant oils colored with fluorescent dyes and method for their use as leak detectors  

SciTech Connect (OSTI)

A leak detectable refrigeration composition is described comprising: (A) a refrigeration liquid selection from the group consisting of: (1) a polyhalogenated hydrocarbon refrigerant; (2) a refrigeration oil selected from the group consisting of naphthenic oils, paraffinic oils, alkylated benzenes, silicones, polyglycols, diesters or triesters of dicarboxylic or tricarboxylic acids, and polyalkyl silicate oils, and (3) a mixture of A(1) and A(2), and (B) a fluorescent dye compound or composition comprising the dye selected from the group consisting of: (1) a fluorescent dye selected from the group consisting of perylene, naphthoxanthene, monocyclic aromatic compounds having an organometallic compound, (2) a solution of fluorescent dye in a solvent, and (3) a mixture of B(1) and B(2). The fluorescent dye compound or composition is soluble in the refrigeration liquid. The concentration of the dye being at least 0.001 grams per 100 grams of the refrigeration liquid.

Parekh, M.

1988-07-19T23:59:59.000Z

363

Early Events in the Fusarium verticillioides-Maize Interaction Characterized by Using a Green Fluorescent Protein-Expressing Transgenic Isolate  

Science Journals Connector (OSTI)

...common maize fungi. Cereal Res. Commun. 25...grown from infected seeds. Plant Dis. 81...Fusarium pathosystem. Cereal Res. Commun. 25...Institute for Cereal Crop Improvement...Proteins genetics metabolism Microscopy, Fluorescence...Roots microbiology Seeds microbiology Transgenes...

Liat Oren; Smadar Ezrati; David Cohen; Amir Sharon

2003-03-01T23:59:59.000Z

364

Study of the correlation between the coal calorific value and coal ash content using X-ray fluorescence analysis  

Science Journals Connector (OSTI)

In this paper we have studied the possibility of determining the chemical elements in coal samples using X-ray fluorescence analysis and have found a relationship between the coal calorific value and its ash cont...

D. Bolortuya; P. Zuzaan; M. V. Gustova

2013-12-01T23:59:59.000Z

365

Energy transfer to a proton-transfer fluorescence probe: Tryptophan to a flavonol in human serum?albumin  

Science Journals Connector (OSTI)

...important targets in situ. For example, flavonols (3-HF, fisetin...IT>in situ. For example, flavonols (3-HF, fisetin, and quercetin) can be used as biologically active fluorescence...

Alexander Sytnik; Igor Litvinyuk

1996-01-01T23:59:59.000Z

366

Probing Ag nanoparticle surface oxidation in contact with (in)organics: an X-ray scattering and fluorescence yield approach  

Science Journals Connector (OSTI)

An advanced characterization approach to spatially resolve the reactions occurring at the interface between silver nanoparticles and a simple aqueous system is presented. Both phase and chemical distributions were obtained from X-ray scattering and fluorescence yield techniques.

Levard, C.

2011-10-06T23:59:59.000Z

367

Micromolding of a Highly Fluorescent Reticular Coordination Polymer: Solvent-Mediated Reconfigurable Polymerization in a Soft Lithographic Mold  

SciTech Connect (OSTI)

Coordination polymerization of pyridine-based ligands and zinc or silver ions was controlled by soft lithographic micromolding in capillaries. The polymer patterns that are produced are highly fluorescent and supramolecularly structured.

Y You; H Yang; J Chung; J Kim; Y Jung; S Park

2011-12-31T23:59:59.000Z

368

Serum proteins, indirect fluorescent antibody titers and complement fixing antibody titers in dexamethasone exacerbated anaplasmosis carrier calves  

E-Print Network [OSTI]

SERUM PROTEINS, INDIRECT FLUORESCENT ANTIBODY TITERS AND COMPLEMENT FIXING ANTIBODY TITERS IN DEXAMETHASONE EXACERBATED ANAPLASMOSIS CARRIER CALVES A Thesis by Lloran Wesley Johnson Submitted to the Graduate College of Texas A%M University... in partial fulfillment of the requirement for the degree of MAS TER 0 F SC I ENC E August 1979 Major Subject: Veterinary Microbiology SERUM PROTEINS, INDIRECT FLUORESCENT ANTIBODY TITERS AND COMPLEMENT FIXING ANTIBODY TITERS IN DEXAMETHASONE EXACERBATED...

Johnson, Lloran Wesley

2012-06-07T23:59:59.000Z

369

Influence of conformational changes in complex molecules on photon statistics of single molecule fluorescence  

SciTech Connect (OSTI)

A single complex molecule with conformational changes (conformations 0 and 2) is considered. When such a molecule is irradiated by cw-laser light it can randomly change the intensity or polarization of its fluorescence due to jumps from one conformation to another. In fact, the molecule manifests itself either like the 0-type or the 2-type emitter. An expression for the matrix s{sub {alpha}{beta}}(t) called the start-stop correlator (waiting time distribution) in which {alpha}=0,2 and {beta}=0,2 is derived. An expression for the matrix p{sub {alpha}{beta}}(t) called the full correlator is derived as well. It determines the density of the probability of finding an event of {alpha} type and an event of {beta} type separated by time interval t. A relation between matrices s{sub {alpha}{beta}}(t) and p{sub {alpha}{beta}}(t) is found. A mathematical expression for the distribution w{sub N}(T) of events measured in time interval T is derived. It is expressed solely via the matrix s{sub {alpha}{beta}}(t). Numerical calculations of the event distribution function for various rates of intra- and interconformational jumps are carried out with the help of the formula for w{sub N}(T) and by the Monte Carlo method. Both methods of the calculation yield identical distributions. Fluctuating fluorescence intensity I(t) for a bin time of 5 ms is calculated for slow and fast interconformational jumps. A relation is found between the autocorrelation function g{sup (2)}(t) of fluorescence measurable in experiments and the matrix p{sub {alpha}{beta}}(t) calculated theoretically.

Osad'ko, I. S. [Institute of Spectroscopy Russian Academy of Sciences (RAS), 142190 Troitsk, Moscow Region (Russian Federation); Moscow Pedagogical State University, 119991 Moscow (Russian Federation); Fedyanin, V. V. [Moscow Pedagogical State University, 119991 Moscow (Russian Federation)

2011-06-15T23:59:59.000Z

370

High resolution x-ray fluorescence spectroscopy - a new technique for site- and spin-selectivity  

SciTech Connect (OSTI)

X-ray spectroscopy has long been used to elucidate electronic and structural information of molecules. One of the weaknesses of x-ray absorption is its sensitivity to all of the atoms of a particular element in a sample. Through out this thesis, a new technique for enhancing the site- and spin-selectivity of the x-ray absorption has been developed. By high resolution fluorescence detection, the chemical sensitivity of K emission spectra can be used to identify oxidation and spin states; it can also be used to facilitate site-selective X-ray Absorption Near Edge Structure (XANES) and site-selective Extended X-ray Absorption Fine Structure (EXAFS). The spin polarization in K fluorescence could be used to generate spin selective XANES or spin-polarized EXAFS, which provides a new measure of the spin density, or the nature of magnetic neighboring atoms. Finally, dramatic line-sharpening effects by the combination of absorption and emission processes allow observation of structure that is normally unobservable. All these unique characters can enormously simplify a complex x-ray spectrum. Applications of this novel technique have generated information from various transition-metal model compounds to metalloproteins. The absorption and emission spectra by high resolution fluorescence detection are interdependent. The ligand field multiplet model has been used for the analysis of K{alpha} and K{beta} emission spectra. First demonstration on different chemical states of Fe compounds has shown the applicability of site selectivity and spin polarization. Different interatomic distances of the same element in different chemical forms have been detected using site-selective EXAFS.

Wang, Xin [Univ. of California, Davis, CA (United States). Dept. of Applied Science]|[Lawrence Berkeley National Lab., CA (United States)

1996-12-01T23:59:59.000Z

371

Development of an Ion-Sensor using Fluorescence Resonance Energy Transfer  

E-Print Network [OSTI]

A method is presented for the sensing of ions by determining the concentration of corresponding salts (KCl, NaCl, MgCl2, CaCl2, FeCl3, FeSO4, AlCl3) in water, based on Fluorescence resonance energy transfer (FRET) process. The principle of the proposed sensor is based on the change of FRET efficiency between two laser dyes Acriflavine and Rhodamine B in presence of different ions (K+, Na+, Mg2+, Ca2+, Fe2+, Fe3+, Al3+). Nanodimensional clay platelet laponite was used to enhance the efficiency of the sensor.

Dey, Dibyendu; Bhattacharjee, D; Hussain, Syed Arshad

2014-01-01T23:59:59.000Z

372

In-Situ Silver Acetylide Silver Nitrate Explosive Deposition Measurements Using X-Ray Fluorescence.  

SciTech Connect (OSTI)

The Light Initiated High Explosive facility utilized a spray deposited coating of silver acetylide - silver nitrate explosive to impart a mechanical shock into targets of interest. A diagnostic was required to measure the explosive deposition in - situ. An X - ray fluorescence spectrometer was deployed at the facility. A measurement methodology was developed to measure the explosive quantity with sufficient accuracy. Through the use of a tin reference material under the silver based explosive, a field calibration relationship has been developed with a standard deviation of 3.2 % . The effect of the inserted tin material into the experiment configuration has been explored.

Covert, Timothy T.

2014-09-01T23:59:59.000Z

373

Glass transition of associated solvents studied by fluorescence measurement of doped chromophores  

Science Journals Connector (OSTI)

The fluorescence lifetime of a triphenylmethane dye, malachite green, doped in three glass-forming associated solvents, 1-propanol, propylene glycol, and glycerol, was measured in a wide temperature range. For each sample three temperature regimes were found in the temperature dependence of the nonradiative relaxation time of malachite green. The lower crossover temperature corresponds to the calorimetric glass transition temperature, and the higher one, 3050 K above the lower one, is attributed to the critical temperature that is predicted by mode-coupling theory. 1996 The American Physical Society.

Jing Yong Ye; Toshiaki Hattori; Hideyuki Inouye; Hiroshi Ueta; Hiroki Nakatsuka; Yoshihiro Maruyama; Mitsuru Ishikawa

1996-04-01T23:59:59.000Z

374

A two-photon fluorescence autocorrelator for a Nd:YLF modelocked laser  

SciTech Connect (OSTI)

In this thesis, I discuss the design and implementation of an autocorrelator for an actively modelocked ND:YLF laser at wavelength 1.054{mu}m. A dye is used to generate a broadband two-photon fluorescence (TPF) signal at 570nm which is the autocorrelation of the laser pulses. Two different techniques are discussed. A colliding pulse scheme can be used to generate a TPF autocorrelation signal as a function of distance, or an interferometer technique can be used to generate an autocorrelation signal as a function of the delay in an interferometer arm. Experimental results are discussed, but they are inconclusive because of difficulties in interpreting the signal.

Kner, P.A.

1991-05-01T23:59:59.000Z

375

Fluorescence emission as a probe to investigate electrochemical polymerization of 9-vinylanthracene  

SciTech Connect (OSTI)

The cationic polymerization of 9-vinylanthracene can be initiated at a transparent SnO/sub 2/ electrode with the application of anodic potentials (E > 1.1 V vs. saturated sodium chloride calomel electrode) in acetonitrile solutions. The excimer emission (emission maximum approx.500 nm) of poly(9-vinyl-anthracene) which is distinguishable from the monomer fluorescence emission (emission maxima 410, 430 nm) has been used to probe the electrochemical polymerization process directly. The in situ spectroelectrochemical technique, which would be useful in obtaining kinetic and mechanistic information of the electropolymerization process, is described.

Kamat, P.V.

1987-07-01T23:59:59.000Z

376

The convergence of quantum-dot-mediated fluorescence resonance energy transfer and  

Science Journals Connector (OSTI)

We present a novel convergence of quantum-dot-mediated fluorescence resonance energy transfer (QD-FRET) and microfluidics, through which molecular interactions were precisely controlled and monitored using highly sensitive quantum-dot-mediated FRET. We demonstrate its potential in studying the kinetics of self-assembly of DNA polyplexes under laminar flow in real time with millisecond resolution. The integration of nanophotonics and microfluidics offers a powerful tool for elucidating the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

Yi-Ping Ho; Hunter H Chen; Kam W Leong; Tza-Huei Wang

2009-01-01T23:59:59.000Z

377

The Multiply Scattering Effect on the Energy Measurement of UHE Cosmic Rays using Atmospheric Fluorescence Technique  

E-Print Network [OSTI]

Point sources in the atmosphere are surrounded by aureole because of atmospheric scattering. The properties of the time-dependent aureole radiance are calculated by use of a Monte Carlo approach and an iterative method. Since the aureole is particularly important in the ultraviolet, which is the region the Ultra-High-Energy (UHE) cosmic ray experiment using the air fluorescence technique like Fly's Eye or High-Resolution-Fly's-Eye(HiRes) are set in. The effect of the multiply scatteing on the energy measurement is studied.

Xingzhi Zhang

2000-04-12T23:59:59.000Z

378

Fluorescent Single-Walled Carbon Nanotube Aerogels in Surfactant-free Environments  

Science Journals Connector (OSTI)

Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Center for Integrated Nanotechnologies, Materials Physics and Applications Division, Polymers and Coatings Group, Materials Science and Technology Division, and ?National High Magnetic Field Laboratory, Los Alamos National Laboratory, Los Alamos New Mexico 87545, United States ... single-walled carbon nanotube; fluorescence; surfactant-free; silica aerogel; nanocomposite; low-temperature ... Thus, during gelation the silica precursor molecules can penetrate the SDS assembly more readily than the DOC coating resulting in weaker PL intensities for the SDS-suspended tubes in contrast to DOC-wrapped tubes. ...

Juan G. Duque; Christopher E. Hamilton; Gautam Gupta; Scott A. Crooker; Jared. J. Crochet; Aditya Mohite; Han Htoon; Kimberly A. DeFriend Obrey; Andrew M. Dattelbaum; Stephen K. Doorn

2011-07-26T23:59:59.000Z

379

2014-10-14 Issuance: Test Procedures Correction for Fluorescent Lamp Ballasts; Notice of Proposed Rulemaking  

Broader source: Energy.gov [DOE]

This document is a pre-publication Federal Register notice of proposed rulemaking regarding test procedures for fluorescent lamp ballasts, as issued by the Deputy Assistant Secretary for Energy Efficiency on October 14, 2014. Though it is not intended or expected, should any discrepancy occur between the document posted here and the document published in the Federal Register, the Federal Register publication controls. This document is being made available through the Internet solely as a means to facilitate the public's access to this document.

380

A Graphene Oxide-Based Fluorescent Biosensor for the Analysis of PeptideReceptor Interactions and Imaging in Somatostatin Receptor Subtype 2 Overexpressed Tumor Cells  

Science Journals Connector (OSTI)

Interestingly, the specific binding of the antibody anti-octreotide (AOC) with FOC competitively releases FOC from the GO surface, leading to the recovery of fluorescence. ... By using this GO-based fluorescent platform, we can detect AOC with a low detection limit of 2 ng/mL. ... The interaction between FOC and its antibody, anti-octreotide (AOC), was investigated with the fluorescence emission spectra of FOC in the presence of AOC. ...

Feng Bianying; Guo Linjie; Wang Lihua; Li Fan; Lu Jianxin; Gao Jimin; Fan Chunhai; Huang Qing

2013-07-18T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

Fluorescence-detected assembly of the signal recognition particle: binding of the two SRP protein heterodimers to SRP RNA is noncooperative  

Science Journals Connector (OSTI)

Fluorescence-detected assembly of the signal recognition particle: binding of the two SRP protein heterodimers to SRP RNA is noncooperative ...

Fabiola Janiak; Peter Walter; Arthur E. Johnson

1992-06-01T23:59:59.000Z

382

High Repetition Rate, LINAC-based Nuclear Resonance Fluorescence FY 2009 Final Report  

SciTech Connect (OSTI)

Nuclear Resonance Fluorescence (NRF), which is possible for nuclei with atomic numbers greater than helium (Z=2), occurs when a nuclear level is excited by resonant absorption of a photon and subsequently decays by reemission of a photon. The excited nuclear states can become readily populated, provided the incident photons energy is within the Doppler-broadened width of the energy level being excited. Utilizing continuous energy photon spectra, as is characteristic of a bremsstrahlung photon beam, as the inspection source, ensures that at least some fraction of the impinging beam will contribute to the population of the excited energy levels in the material of interest. Upon de-excitation, either to the ground state or to a lower-energy excited state, the emitted fluorescence photons energy will correspond to the energy difference between the excited state and the state to which it decays. As each isotope inherently contains unique nuclear energy levels, the NRF states for each isotope are also unique. By exploiting this phenomenon, NRF photon detection provides a well-defined signature for identifying the presence of individual nuclear species. This report summarizes the second year (Fiscal Year [FY] 2009) of a collaborative research effort between Idaho National Laboratory, Idaho State Universitys Idaho Accelerator Center, and Pacific Northwest National Laboratory. This effort focused on continuing to assess and optimize NRF-based detection techniques utilizing a slightly modified, commercially available, pulsed medical electron accelerator.

Mathew Kinlaw; Scott Watson; James Johnson; Alan Hunt; Heather Seipel; Edward Reedy

2009-10-01T23:59:59.000Z

383

3D optical sectioning with a new hyperspectral confocal fluorescence imaging system.  

SciTech Connect (OSTI)

A novel hyperspectral fluorescence microscope for high-resolution 3D optical sectioning of cells and other structures has been designed, constructed, and used to investigate a number of different problems. We have significantly extended new multivariate curve resolution (MCR) data analysis methods to deconvolve the hyperspectral image data and to rapidly extract quantitative 3D concentration distribution maps of all emitting species. The imaging system has many advantages over current confocal imaging systems including simultaneous monitoring of numerous highly overlapped fluorophores, immunity to autofluorescence or impurity fluorescence, enhanced sensitivity, and dramatically improved accuracy, reliability, and dynamic range. Efficient data compression in the spectral dimension has allowed personal computers to perform quantitative analysis of hyperspectral images of large size without loss of image quality. We have also developed and tested software to perform analysis of time resolved hyperspectral images using trilinear multivariate analysis methods. The new imaging system is an enabling technology for numerous applications including (1) 3D composition mapping analysis of multicomponent processes occurring during host-pathogen interactions, (2) monitoring microfluidic processes, (3) imaging of molecular motors and (4) understanding photosynthetic processes in wild type and mutant Synechocystis cyanobacteria.

Nieman, Linda T.; Sinclair, Michael B.; Davidson, George S.; Van Benthem, Mark Hilary; Haaland, David Michael; Timlin, Jerilyn Ann; Sasaki, Darryl Yoshio; Bachand, George David; Jones, Howland D. T.

2007-02-01T23:59:59.000Z

384

Laser induced coal fluorescence. Tenth quarterly technical progress report, January 1-March 31, 1985  

SciTech Connect (OSTI)

The work performed during this period consisted of the completion of time resolved data acquisition and reduction of New Albany shale rank series, preliminary testing of a new deconvolution method, and the writing of additional software. The data acquisition consisted of 21 decay curves, one taken every 10nm over the fluorescence emission range of 450 to 650nm for each alginite maceral investigated. Approximately five alginite macerals were examined from each of the thirteen samples making a total of 61 macerals (1281 decay curves). The decay data were deconvoluted, resulting in decay times and percentages of fluorescing compounds as a function of wavelength. Because of the massive amount of data we are able to collect in a relatively short time and the fact that the current iterative deconvolution method requires a relatively large amount of time, it became imperative that a new deconvolution method be investigated. The new method was suggested by Dr. Robert Zitter, and, for the purpose of this report, is tentatively named the ''Zitter Method.'' The software written during this period consisted primarily of additional data storage and presentation routines. 14 figs., 2 tabs.

Borst, W.L.

1985-01-01T23:59:59.000Z

385

Validation of the Real and Simulated Data of the Pierre Auger Fluorescence Telescopes  

E-Print Network [OSTI]

The fluorescence detector (FD) of the Pierre Auger Observatory is currently operating 18 fluorescence telescopes of the 24 that will be employed in the completed detector. These telescopes, grouped in 4 eyes each consisting of 6 telescopes, measure the longitudinal profile of cosmic ray showers with a 14% duty cycle. The reconstruction capability and triggering efficiency have been studied using a complete simulation and reconstruction production chain, employing both simulated CORSIKA showers and parameterised Gaisser-Hillas profiles. The propagation through the atmosphere and the detector response are taken into account and simulated in detail. These simulated data have been generated in a preliminary analysis using the method of importance sampling to efficiently cover the energy region of 0.3 - 300 EeV, various shower geometries and impact points and different primary particles. The distributions of observables have then been investigated in both real and simulated data, facilitating the validation of the reconstruction and simulation software. Comparisons of real and simulated data are discussed and used to assess their impact on the data analysis.

The Pierre Auger Collaboration; L. Perrone

2005-07-20T23:59:59.000Z

386

Fluorescent protein pair emit intracellular FRET signal suitable for FACS screening  

SciTech Connect (OSTI)

The fluorescent proteins ECFP and HcRed were shown to give an easily resolved FRET-signal when expressed as a fusion inside mammalian cells. HeLa-tat cells expressing ECFP, pHcRed, or the fusion protein pHcRed-ECFP were analyzed by flow cytometry after excitation of ECFP. Cells expressing HcRed-ECFP, or ECFP and HcRed, were mixed and FACS-sorted for FRET positive cells: HcRed-ECFP cells were greatly enriched (72 times). Next, cloned human antibodies were fused with ECFP and expressed anchored to the ER membrane. Their cognate antigens (HIV-1 gp120 or gp41) were fused to HcRed and co-expressed in the ER. An increase of 13.5 {+-} 1.5% (mean {+-} SEM) and 8.0 {+-} 0.7% in ECFP fluorescence for the specific antibodies reacting with gp120 or gp41, respectively, was noted after photobleaching. A positive control (HcRed-ECFP) gave a 14.8 {+-} 2.6% increase. Surprisingly, the unspecific antibody (anti-TT) showed 12.1 {+-} 1.1% increase, possibly because overexpression in the limited ER compartment gave false FRET signals.

Johansson, Daniel X. [Karolinska Institutet, Department of Medicine, Karolinska Hospital, 171 76 Stockholm (Sweden); Brismar, Hjalmar [Karolinska Institutet, Department of Woman and Child Health, Karolinska Hospital, 171 76 Stockholm (Sweden); Royal Institute of Technology, Department of Cell Physics, KTH/SCFAB, 106 91 Stockholm (Sweden); Persson, Mats A.A. [Karolinska Institutet, Department of Medicine, Karolinska Hospital, 171 76 Stockholm (Sweden)]. E-mail: mats.persson@ki.se

2007-01-12T23:59:59.000Z

387

Isotopic imaging via nuclear resonance fluorescence with laser-based Thomson radiation  

DOE Patents [OSTI]

The present invention utilizes novel laser-based, high-brightness, high-spatial-resolution, pencil-beam sources of spectrally pure hard x-ray and gamma-ray radiation to induce resonant scattering in specific nuclei, i.e., nuclear resonance fluorescence. By monitoring such fluorescence as a function of beam position, it is possible to image in either two dimensions or three dimensions, the position and concentration of individual isotopes in a specific material configuration. Such methods of the present invention material identification, spatial resolution of material location and ability to locate and identify materials shielded by other materials, such as, for example, behind a lead wall. The foundation of the present invention is the generation of quasimonochromatic high-energy x-ray (100's of keV) and gamma-ray (greater than about 1 MeV) radiation via the collision of intense laser pulses from relativistic electrons. Such a process as utilized herein, i.e., Thomson scattering or inverse-Compton scattering, produces beams having diameters from about 1 micron to about 100 microns of high-energy photons with a bandwidth of .DELTA.E/E of approximately 10E.sup.-3.

Barty, Christopher P. J. (Hayward, CA); Hartemann, Frederic V. (San Ramon, CA); McNabb, Dennis P. (Alameda, CA); Pruet, Jason A. (Brentwood, CA)

2009-07-21T23:59:59.000Z

388

A high-resolution large-acceptance analyzer for X-ray fluorescence and Raman spectroscopy  

SciTech Connect (OSTI)

A newly designed multi-crystal X-ray spectrometer and its applications in the fields of X-ray fluorescence and X-ray Raman spectroscopy are described. The instrument is based on 8 spherically curved Si crystals, each with a 3.5 inch diameter form bent to a radius of 86 cm. The crystals are individually aligned in the Rowland geometry capturing a total solid angle of 0.07 sr. The array is arranged in a way that energy scans can be performed by moving the whole instrument, rather than scanning each crystal by itself. At angles close to back scattering the energy resolution is between 0.3 and 1 eV depending on the beam dimensions at the sample. The instrument is mainly designed for X-ray absorption and fluorescence spectroscopy of transition metals in dilute systems such as metalloproteins. First results of the Mn K{beta} (3p -> 1s) emission in photosystem II are shown. An independent application of the instrument is the technique of X-ray Raman spectroscopy which can address problems similar to those in traditional soft X-ray absorption spectroscopies, and initial results are presented.

Bergmann, Uwe; Cramer, Stephen P.

2001-08-02T23:59:59.000Z

389

Laser-Induced Fluorescence Detection in High-Throughput Screening of Heterogeneous Catalysts and Single Cells Analysis  

SciTech Connect (OSTI)

Laser-induced fluorescence detection is one of the most sensitive detection techniques and it has found enormous applications in various areas. The purpose of this research was to develop detection approaches based on laser-induced fluorescence detection in two different areas, heterogeneous catalysts screening and single cell study. First, the author introduced laser-induced imaging (LIFI) as a high-throughput screening technique for heterogeneous catalysts to explore the use of this high-throughput screening technique in discovery and study of various heterogeneous catalyst systems. This scheme is based on the fact that the creation or the destruction of chemical bonds alters the fluorescence properties of suitably designed molecules. By irradiating the region immediately above the catalytic surface with a laser, the fluorescence intensity of a selected product or reactant can be imaged by a charge-coupled device (CCD) camera to follow the catalytic activity as a function of time and space. By screening the catalytic activity of vanadium pentoxide catalysts in oxidation of naphthalene, they demonstrated LIFI has good detection performance and the spatial and temporal resolution needed for high-throughput screening of heterogeneous catalysts. The sample packing density can reach up to 250 x 250 subunits/cm{sup 2} for 40-{micro}m wells. This experimental set-up also can screen solid catalysts via near infrared thermography detection. In the second part of this dissertation, the author used laser-induced native fluorescence coupled with capillary electrophoresis (LINF-CE) and microscope imaging to study the single cell degranulation. On the basis of good temporal correlation with events observed through an optical microscope, they have identified individual peaks in the fluorescence electropherograms as serotonin released from the granular core on contact with the surrounding fluid.

Hui Su

2001-05-25T23:59:59.000Z

390

Cold Fluorescent Light as Major Inducer of Lipid Oxidation in Soybean Oil Stored at Household Conditions for Eight Weeks  

Science Journals Connector (OSTI)

Soybean oil stored in the dark for 56 days showed an increase of the peroxide value by 124 0.62% (p = 0.006), whereas exposure of the oil to light in a cycle of 12 h light alternating with 12 h darkness for 56 days led to a rise of the peroxide value by 1473 1.79% (p ? 0.001). ... Photosensitized oxidation and autoxidation of vegetable oils lead to the formation of a broad range of oxidation products. ... To investigate the influence of cold fluorescent light, bulbs were chosen that emit electromagnetic irradiation in the cold fluorescent range thereby mimicking the storage condition in a typical household. ...

Marc Pignitter; Klaus Stolze; Stephanie Gartner; Bettina Dumhart; Christiane Stoll; Georg Steiger; Klaus Kraemer; Veronika Somoza

2014-02-18T23:59:59.000Z

391

Reversible Self-Assembly of Entrapped Fluorescent Gelators in Polymerized Styrene Gel Matrix: Erasable Thermal Imaging via Recreation of Supramolecular Architectures  

Science Journals Connector (OSTI)

Reversible Self-Assembly of Entrapped Fluorescent Gelators in Polymerized Styrene Gel Matrix: Erasable Thermal Imaging via Recreation of Supramolecular Architectures ... A solvent-vapor-controlled recreation of the self-assembly of a fluorescent organogelator within a polymer matrix and its application in erasable secret documentation has not been reported previously. ...

Sampath Srinivasan; Palathingal A. Babu; Sankarapillai Mahesh; Ayyappanpillai Ajayaghosh

2009-10-01T23:59:59.000Z

392

THE JOURNAL OF CHEMICAL PHYSICS VOLUME 57, NUMBER 3 1 AUGUST 1972 Laser-Induced Fluorescence: A Method to Measure the Internal State Distribution of  

E-Print Network [OSTI]

THE JOURNAL OF CHEMICAL PHYSICS VOLUME 57, NUMBER 3 1 AUGUST 1972 Laser-Induced Fluorescence a pulsed (2-10 nsec duration), tunable dye laser3 passes through the reaction zone. As the wave- length 20 nsec the opening of the electronic gate. The BaO fluorescence rate versus laser wavelength

Zare, Richard N.

393

Detection of Listeria monocytogenes from a Model Food by Fluorescence Resonance Energy Transfer-Based PCR with an Asymmetric Fluorogenic Probe Set  

Science Journals Connector (OSTI)

...Proc. Natl. Acad. Sci. USA 88: 7276-7280. 11 Ivarsson...and detection of DNA based on energy transfer. Nucleic Acids Res...North Carolina 27695-7624, USA. | Evaluation Studies Journal...Probes Fluorescence Resonance Energy Transfer Fluorescent Dyes Heat-Shock...

Kai Koo; Lee-Ann Jaykus

2003-02-01T23:59:59.000Z

394

Cellular Dynamics Visualized in Live Cells in Vitro and in Vivo by Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein Expression  

Science Journals Connector (OSTI)

...Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein...Kanazawa, Ishikawa, Japan; and 4 The Salk Institute...that enables real-time nuclear-cytoplasmic dynamics...fluorescence microscopy. Nuclear Cytoplasmic Dynamics...VT) with a 50-W power supply. Images were...

Norio Yamamoto; Ping Jiang; Meng Yang; Mingxu Xu; Kensuke Yamauchi; Hiroyuki Tsuchiya; Katsuro Tomita; Geoffrey M. Wahl; Abdool R. Moossa; and Robert M. Hoffman

2004-06-15T23:59:59.000Z

395

Ratiometric Analysis of Single-Molecule Fluorescence Resonance Energy Transfer Using Logical Combinations of Threshold Criteria: A Study of 12-mer DNA  

E-Print Network [OSTI]

Ratiometric Analysis of Single-Molecule Fluorescence Resonance Energy Transfer Using LogicalEW United Kingdom ReceiVed: NoVember 5, 1999; In Final Form: March 13, 2000 Single-molecule can be readily distinguished by this single-molecule method. Fluorescence lifetimes and anisotropy

Wallace, Mark

396

Real-Time Proteomics (RTP) by Single Molecule Fluorescence Microscopy Abstract We propose to develop a new technology for live cell gene expression -Protein Synthesis Measurement (PSM).  

E-Print Network [OSTI]

Real-Time Proteomics (RTP) by Single Molecule Fluorescence Microscopy Abstract We propose use single molecule fluorescence resonance energy transfer (smFRET) to obtain a PSM signal from system that will translate a library of C. elegans mRNA messages and the signals produced from single

Kim, Junhyong

397

PhotochemisfvondPhofobiology. 1971 . Vol. 14,pp. 667-682. PergamonPress. Printed in Great Britain FLUORESCENCE INDUCTION IN THE RED ALGA  

E-Print Network [OSTI]

FLUORESCENCE INDUCTION IN THE RED ALGA PORPHYRIDIUM CRUENTUM P. MOHANTY, G. PAPAGEORGIOU* and GOVINDJEE in the red alga Porphyridium cruentum. Both the fast and the slow fluorescence yield changes are affected algae both in the fast (sec) and in the slow (min) region (see Refs. [l-31). Characteristic points

Govindjee

398

Lunar X-ray fluorescence observations by the Chandrayaan-1 X-ray Spectrometer (C1XS): Results from the nearside southern highlands  

E-Print Network [OSTI]

Lunar X-ray fluorescence observations by the Chandrayaan-1 X-ray Spectrometer (C1XS): Results from Spectroscopy a b s t r a c t The Chandrayaan-1 X-ray Spectrometer (C1XS) flown on-board the first Indian lunar mission Chan- drayaan-1, measured X-ray fluorescence spectra during several episodes of solar flares

Wieczorek, Mark

399

Integrated starting and running amalgam assembly for an electrodeless fluorescent lamp  

DOE Patents [OSTI]

An integrated starting and running amalgam assembly for an electrodeless SEF fluorescent lamp includes a wire mesh amalgam support constructed to jointly optimize positions of a starting amalgam and a running amalgam in the lamp, thereby optimizing mercury vapor pressure in the lamp during both starting and steady-state operation in order to rapidly achieve and maintain high light output. The wire mesh amalgam support is constructed to support the starting amalgam toward one end thereof and the running amalgam toward the other end thereof, and the wire mesh is rolled for friction-fitting within the exhaust tube of the lamp. The positions of the starting and running amalgams on the wire mesh are jointly optimized such that high light output is achieved quickly and maintained, while avoiding any significant reduction in light output between starting and running operation.

Borowiec, Joseph Christopher (Schenectady, NY); Cocoma, John Paul (Clifton Park, NY); Roberts, Victor David (Burnt Hills, NY)

1998-01-01T23:59:59.000Z

400

A Fluorescence Displacement Assay for Antidepressant Drug Discovery Based on Ligand-Conjugated Quantum Dots  

SciTech Connect (OSTI)

The serotonin (5-hydroxytryptamine, 5-HT) transporter (SERT) protein plays a central role in terminating 5-HT neurotransmission and is the most important therapeutic target for the treatment of major depression and anxiety disorders. We report an innovative, versatile, and target-selective quantum dot (QD) labeling approach for SERT in single Xenopus oocytes that can be adopted as a drug-screening platform. Our labeling approach employs a custom-made, QD-tagged indoleamine derivative ligand, IDT318, that is structurally similar to 5-HT and accesses the primary binding site with enhanced human SERT selectivity. Incubating QD-labeled oocytes with paroxetine (Paxil), a high-affinity SERT-specific inhibitor, showed a concentration- and time-dependent decrease in QD fluorescence, demonstrating the utility of our approach for the identification of SERT modulators. Furthermore, with the development of ligands aimed at other pharmacologically relevant targets, our approach may potentially form the basis for a multitarget drug discovery platform.

Chang, Jerry [Vanderbilt University; Tomlinson, Ian [Oak Ridge National Laboratory (ORNL); Warnement, Michael [Vanderbilt University; Iwamoto, Hideki [Vanderbilt University

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

BIOCOMPATIBLE FLUORESCENT MICROSPHERES: SAFE PARTICLES FOR MATERIAL PENETRATION STUDIES (TAS Review Brief)  

SciTech Connect (OSTI)

The use of biocompatible polymer microspheres has been proposed for use in drug delivery, dispersal model verification and as a surrogate for threat agents in instrument validation. Modifying the production methods used in drug delivery for aerosol production should allow for low cost bio-weapon simulants. The ability to have a polymer sphere with a broad range of property tuning will allow for safe low cost use for homeland security and defense applications. Three main challenges are present in the production of such spheres. The first challenge is the reproduction of the fluorescent and other chemical properties of interest. Secondly, the particles must have the size and charge attributes needed for the specific application and testing. Finally, in order to reproduce testing and evaluation criteria a rapid, reliable, scalable and low cost production method must be available.

Farquar, G; Leif, R

2009-07-22T23:59:59.000Z

402

Inferring Black Carbon Concentrations in Particulate Organic Matter by Observing Pyrene Fluorescence Losses  

Science Journals Connector (OSTI)

Inferring Black Carbon Concentrations in Particulate Organic Matter by Observing Pyrene Fluorescence Losses ... For example, most atmospheric scientists are interested in the blackness of aerosols and the resultant effect on the radiative heat balance (3, 7), while oceanographers and soil scientists are interested in the refractory properties of BC in soils and sediments influencing its role in carbon cycling (5). ... Studies since the late 1970s and early 1980s have suggested that absorption into biogenic and diagenetic organic matter is a key process controlling the fate and effects of hydrophobic organic pollutants (8, 9), and this process has been described using a linear sorption model where Kd is the solid?water distribution coefficient (L/kgsolid), and this parameter is estimated using the product of the total organic carbon (TOC) fraction (fTOC, kgTOC/kgsediment) and the TOC-normalized distribution coefficient (KTOC, L/kgTOC). ...

D. Xanat Flores-Cervantes; Christopher M. Reddy; Philip M. Gschwend

2009-05-29T23:59:59.000Z

403

High resolution laser induced fluorescence Doppler velocimetry utilizing saturated absorption spectroscopy  

SciTech Connect (OSTI)

A high resolution laser induced fluorescence (LIF) system has been developed to measure the flow velocity field of neutral particles in an electron-cyclotron-resonance argon plasma. The flow velocity has been determined by the Doppler shift of the LIF spectrum, which is proportional to the velocity distribution function. Very high accuracy in velocity determination has been achieved by installing a saturated absorption spectroscopy unit into the LIF system, where the absolute value and scale of laser wavelength are determined by using the Lamb dip and the fringes of a Fabry-Perot interferometer. The minimum detectable flow velocity of a newly developed LIF system is {+-}2 m/s, and this performance remains unchanged in a long-time experiment. From the radial measurements of LIF spectra of argon metastable atoms, it is found that there exists an inward flow of neutral particles associated with neutral depletion.

Aramaki, Mitsutoshi [Department of Electrical Engineering and Computer Science, Nagoya University, Nagoya 464-8603 (Japan); Ogiwara, Kohei; Etoh, Shuzo [Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Kasuga 816-8580 (Japan); Yoshimura, Shinji [National Institute for Fusion Science, Toki 509-5292 (Japan); Tanaka, Masayoshi Y. [Department of Advanced Energy Engineering Science, Kyushu University, Kasuga 816-8580 (Japan)

2009-05-15T23:59:59.000Z

404

Competing decay-channel fluorescence, dissociation, and ionization in superexcited levels of H{sub 2}  

SciTech Connect (OSTI)

The absolute cross sections for the competing decay-channel fluorescence, dissociation, and ionization of photoexcited long-lived superexcited H{sub 2} molecular levels have been measured from the ionization threshold of H{sub 2} up to the H(1s) + H(n=3) dissociation limit. The total and partial natural widths of these levels have been determined. Good agreement is found with first principles calculations carried out with the multichannel quantum defect theory for excited levels of {sup 1{Pi}}{sub u}{sup -} symmetry, which represent 70% of the levels studied experimentally. The calculations reproduce the balance between the competing decay processes as well as its substantial evolution from level to level.

Glass-Maujean, M. [Laboratoire de Physique Moleculaire pour l'Atmosphere et l'Astrophysique, Universite Pierre et Marie Curie, 4 place Jussieu, F-75252 Paris Cedex 5 (France); Jungen, Ch. [Laboratoire Aime Cotton, Centre National de la Recherche Scientifique, Batiment 505, Universite de Paris-Sud, F-91405 Orsay (France); Department of Physics and Astronomy, University College London, London WC1E 6BT (United Kingdom); Reichardt, G.; Balzer, A. [BESSY II, Albert-Einstein-Strasse 15, D-12489 Berlin (Germany); Schmoranzer, H. [Fachbereich Physik, Technische Universitaet Kaiserslautern, D-67653 Kaiserslautern (Germany); Ehresmann, A.; Haar, I.; Reiss, P. [Institute of Physics and Interdisciplinary Nanostructure Science and Technology, Heinrich-Plett-Strasse 40, Universitaet Kassel, D-34132 Kassel (Germany)

2010-12-15T23:59:59.000Z

405

Transmission Nuclear Resonance Fluorescence Measurements of 238U in Thick Targets  

SciTech Connect (OSTI)

Transmission nuclear resonance fluorescence measurements were made on targets consisting of Pb and depleted U with total areal densities near 86 g/cm2. The 238U content n the targets varied from 0 to 8.5percent (atom fraction). The experiment demonstrates the capability of using transmission measurements as a non-destructive technique to identify and quantify the presence of an isotope in samples with thicknesses comparable to he average thickness of a nuclear fuel assembly. The experimental data also appear to demonstrate the process of notch refilling with a predictable intensity. Comparison of measured spectra to previous backscatter 238U measurements indicates general agreement in observed excited states. Two new 238U excited states and possibly a third state have also been observed.

Quiter, Brian J.; Ludewigt, Bernhard A.; Mozin, Vladimir V.; Wilson, Cody; Korbly, Steve

2010-08-31T23:59:59.000Z

406

High Repetition Rate, LINAC-Based Nuclear Resonance Fluorescence FY 2008 Final Report  

SciTech Connect (OSTI)

This summarizes the first year of a multi-laboratory/university, multi-year effort focusing on high repetition rate, pulsed LINAC-based nuclear resonance fluorescence (NRF) measurements. Specifically, this FY2008 effort centered on experimentally assessing NRF measurements using pulsed linear electron accelerators, operated at various repetition rates, and identifying specific detection requirements to optimize such measurements. Traditionally, interest in NRF as a detection technology, which continues to receive funding from DHS and DOE/NA-22, has been driven by continuous-wave (CW), Van de Graff-based bremsstrahlung sources. However, in addition to the relatively sparse present-day use of Van de Graff sources, only limited NRF data from special nuclear materials has been presented; there is even less data available regarding shielding effects and photon source optimization for NRF measurements on selected nuclear materials.

Scott M Watson; Mathew T Kinlaw; James L Jones; Alan W. Hunt; Glen A. Warren

2008-12-01T23:59:59.000Z

407

Probing tribological properties of waxy oil in pipeline pigging with fluorescence technique  

Science Journals Connector (OSTI)

Abstract Tribological behavior of waxy oil (wax-particles-in-oil mixture) under a sliding soft contact in pipeline pigging process has been investigated using fluorescence technique. Results are reported for a soft line contact between an elastomer cylinder and a glass plate, which is lubricated by a model waxoil system. It was found that, for waxy oil, the wax particles were entrapped at inlet region and pass through the contact region. The study revealed that the pigging efficiency was a function of Youngs module in rubber and the different lubricated soft contacts in pipeline pigging could be in situ observed. Experimental results indicated that the efficiency of pigging process decreased with the increasing degree of wear in sealing disc and was determined by the flow property of waxy oil.

Gui-Bin Tan; De-Guo Wang; Shu-Hai Liu; Si-Wei Zhang

2014-01-01T23:59:59.000Z

408

Fabrication of fluorescent composite with ultrafast aqueous synthesized high luminescent CdTe quantum dots  

SciTech Connect (OSTI)

Without precursor preparation, inert gas protection and enormous amount of additives and reductants, CdTe quantum dots (QDs) can be rapidly synthesized with high quality. A 600 nm photoluminescence peak wavelength could be obtained within 1 hour's refluxing through minimal addition of 1,2-diaminoethane (DAE). The theoretical design for the experiments are illustrated and further proved by the characterization results with different concentrations and reagents. On the other hand, generation of CdTe QDs was found even under room temperature by applying droplet quantity of DAE. This indicates that QDs can be synthesized with simply a bottle and no enormous additives required. The QDs were mixed into the epoxy matrix through solution casting method with cetyltrimethylammonium (CTA) capping for phase transfer. The acquired epoxy based nanocomposite exhibits good transparency, compatibility and fluorescence.

Zhang, Lei, E-mail: mejswu@ust.hk; Chen, Haibin, E-mail: mejswu@ust.hk, E-mail: mejswu@ust.hk; Wu, Jingshen, E-mail: mejswu@ust.hk, E-mail: mejswu@ust.hk [Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Hong Kong and Fok Ying Tung Graduate School, The Hong Kong University of Science and Technology (Hong Kong); Bi, Xianghong, E-mail: takubatch@gmail.com [Fok Ying Tung Graduate School, The Hong Kong University of Science and Technology (Hong Kong)

2014-05-15T23:59:59.000Z

409

Effect of helium addition on discharge characteristics in a flat fluorescent lamp  

SciTech Connect (OSTI)

The discharge characteristics of a multielectrode dual coplanar in a mercury-free flat fluorescent lamp were investigated using brightness-efficiency measurement and the infrared (IR) spectrum and intensified charge coupled device (ICCD) characteristics. The level of brightness was above 14 900 cd/m{sup 2} under the conditions of neon--50% xenon-8% He gas composition, 150 Torr pressure, and 20 kHz alternating current pulse. The ICCD results revealed a faster and wider discharge with a Ne-50% Xe-8% He gas composition. The effect of adding helium (He) to Ne-50% Xe revealed a faster peak emission, as confirmed by ICCD images. From the gated IR emission spectrum, the intensity ratio of I{sub 823nm}/I{sub 828nm} was {approx}8% higher with Ne-50% Xe-8% He than with Ne-50% Xe under the same pressure and applied voltage conditions.

Lee, Seong-Eui; Lee, Ho-Nyeon; Park, Hyoung-Bin; Lee, Kyo Sung; Choi, Kyung Cheol [Materials and Devices Research Center, Samsung Advanced Institute of Technology, Suwon 440-600 (Korea, Republic of); Department of Electronics Engineering, Sejong University, Seoul 143-747 (Korea, Republic of); Department of Electrical Engineering and Computer Science, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701 (Korea, Republic of)

2005-11-01T23:59:59.000Z

410

Integrating fluorometer for the measurement of chlorophyll fluorescence induction in intact plants  

Science Journals Connector (OSTI)

An economical device for monitoring the integrated chlorophyll fluorescence emission of plant material is described. The system which uniquely incorporates an integrating sphere light source photographic shutter optical filters and a photodetector is applicable to intact plants whole leaves or other materials. It is noninvasive and a single sample may be repeatedly tested over time intervals of minutes to months. Data obtained provide information about the sample size (i.e. leaf area total chlorophyll content) and the photosynthetic activity of the sample. Samples of from a few mm2 to several cm2 can be accommodated depending on the diameter of the integrating sphere and excitation light source intensity. The device should be of interest to workers in plant breeding genetic engineering tissue culture horticulture herbicides pollution pathology and environmental stress.

Peter Toivonen; William Vidaver

1984-01-01T23:59:59.000Z

411

Chapter 9 - Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella  

Science Journals Connector (OSTI)

Abstract The eukaryotic flagellum is host to a variety of dynamic behaviors, including flagellar beating, the motility of glycoproteins in the flagellar membrane, and intraflagellar transport (IFT), the bidirectional traffic of protein particles between the flagellar base and tip. IFT is of particular interest, as it plays integral roles in flagellar length control, cell signaling, development, and human disease. However, our ability to understand dynamic flagellar processes such as IFT is limited in large part by the fidelity with which we can image these behaviors in living cells. This chapter introduces the application of total internal reflection fluorescence (TIRF) microscopy to visualize the flagella of Chlamydomonas reinhardtii. The advantages and challenges of TIRF are discussed in comparison to confocal and differential interference contrast techniques. This chapter also reviews current IFT insights gleaned from TIRF microscopy of Chlamydomonas and provides an outlook on the future of the technique, with particular emphasis on combining TIRF with other emerging imaging technologies.

Benjamin D. Engel; Karl-Ferdinand Lechtreck; Tsuyoshi Sakai; Mitsuo Ikebe; George B. Witman; Wallace F. Marshall

2009-01-01T23:59:59.000Z

412

Synchrotron x-ray fluorescence and extended x-ray absorption fine structure analysis  

SciTech Connect (OSTI)

The advent of dedicated synchrotron radiation sources has led to a significant increase in activity in many areas of science dealing with the interaction of x-rays with matter. Synchrotron radiation provides intense, linearly polarized, naturally collimated, continuously tunable photon beams, which are used to determine not only the elemental composition of a complex, polyatomic, dilute material but also the chemical form of the elements with improved accuracy. Examples of the application of synchrotron radiation include experiments in synchrotron x-ray fluorescence (SXRF) analysis and extended x-ray absorption fine structure (EXAFS) analysis. New synchrotron radiation x-ray microprobes for elemental analysis in the parts per billion range are under construction at several laboratories. 76 references, 24 figures.

Chen, J.R.; Gordon, B.M.; Hanson, A.L.; Jones, K.W.; Kraner, H.W.; Chao, E.C.T.; Minkin, J.A.

1984-01-01T23:59:59.000Z

413

Graphene as a Substrate To Suppress Fluorescence in Resonance Raman Spectroscopy  

E-Print Network [OSTI]

Resonance Raman spectroscopy (RRS), which can enhance Raman signal by 107 times than normal Raman spectroscopy, is a powerful approach to characterize structures of chemicals (especially biomolecules) at low concentrations.1-4 However, fluorescence (FL) background is a major obstacle in RRS because the FL cross section (?10-16 cm2) is much larger than the RRS cross section (?10-22 cm2).3 Several approaches, such as ultraviolet RRS (UV-RRS),1 time-resolved Raman detection,5,6 femtosecond broadband stimu-lated Raman spectroscopy (FSRS),7 and coherent anti-Stokes Raman spectroscopy (CARS),8 have been used to suppress or reject FL background in RRS. However, these approaches need expensive and complex equipments and have other limitations, such as sample degradation in UV-RRS. Surface-enhanced Raman spectroscopy (SERS) is another powerful approach to characterize structures of chemicals at extremely low concentrations or even at the single

Liming Xie; Xi Ling; Yuan Fang; Jin Zhang; Zhongfan Liu

2009-01-01T23:59:59.000Z

414

The reproducibility of [sup 109]Cd-based X-ray fluorescence measurements of bone lead  

SciTech Connect (OSTI)

We assessed the reproducibility of X-ray fluorescence-based lead measurements from multiple measurements made on a low-concentration plaster of paris phantom and in five subjects measured five times on two occasions. Over a 6-month period, 220 measurements of the same phantom were obtained and showed a standard deviation of 1.29 [mu]g Pb (g plaster of paris)[sup [minus]1]. The two sets of in vivo measurements were made 10 months apart and revealed a mean standard deviation of 3.4 [mu]Pb (g bone mineral)[sup [minus]1] and 5.1 [mu]g Pb (g bone mineral)[sup [minus]1] for males and females, respectively. Our measured standard deviation exceeded by 20-30% the calculated standard deviation associated with a single measurement both in the phantom and in subjects. This indicates that some variance is introduced during the measurement process. Operator learning and consistency significantly minimized this increased variability. Measured lead concentrations of the left and right tibia in 14 subjects showed no significant differences between legs. As a result, either tibia can be sampled and compared over time. The levels of reproducibility we report here mean that X-ray fluorescence-based determinations of bone lead concentrations are reliable both over the short and long term. Thus, reasonably sized confidence intervals can be placed on detected changes in concentration and should permit acquisition of longitudinal data within a reasonable length of time. 19 refs., 1 fig., 6 tabs.

Gordon, C.L. (Chedoke-McMaster Hospitals, Hamilton, Ontario (Canada)); Webber, C.E.; Chettle, D.R. (McMaster Univ., Hamilton, Ontario (Canada))

1994-08-01T23:59:59.000Z

415

Portable X-ray fluorescence in the assessment of rare earth element-enriched sedimentary phosphate deposits  

Science Journals Connector (OSTI)

...fluorescence in the assessment of rare earth element-enriched sedimentary...considered as potential sources of rare earth elements (REEs) and...phosphate deposit|pulps|rare earth elements|recalibration...into effluents associated with phosphogypsum stockpiles and could pose a...

G.J. Simandl; R. Fajber; S. Paradis

416

Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design  

SciTech Connect (OSTI)

The genetically encoded calcium indicator GCaMP2 shows promise for neural network activity imaging, but is currently limited by low signal-to-noise ratio. We describe x-ray crystal structures as well as solution biophysical and spectroscopic characterization of GCaMP2 in the calcium-free dark state, and in two calcium-bound bright states: a monomeric form that dominates at intracellular concentrations observed during imaging experiments and an unexpected domain-swapped dimer with decreased fluorescence. This series of structures provides insight into the mechanism of Ca{sup 2+}-induced fluorescence change. Upon calcium binding, the calmodulin (CaM) domain wraps around the M13 peptide, creating a new domain interface between CaM and the circularly permuted enhanced green fluorescent protein domain. Residues from CaM alter the chemical environment of the circularly permuted enhanced green fluorescent protein chromophore and, together with flexible inter-domain linkers, block solvent access to the chromophore. Guided by the crystal structures, we engineered a series of GCaMP2 point mutants to probe the mechanism of GCaMP2 function and characterized one mutant with significantly improved signal-to-noise. The mutation is located at a domain interface and its effect on sensor function could not have been predicted in the absence of structural data.

Akerboom, Jasper; Velez Rivera, Jonathan D.; Rodriguez Guilbe, Mara M.; Alfaro Malav, Elisa C.; Hernandez, Hector H.; Tian, Lin; Hires, S. Andrew; Marvin, Jonathan S.; Looger, Loren L.; Schreiter, Eric R.; (MIT); (Puerto Rico); (HHMI)

2009-03-16T23:59:59.000Z

417

Comparison of a semiautomated fluorescent immunoassay system and indirect immunofluorescence for detection of antinuclear antibodies in human serum.  

Science Journals Connector (OSTI)

...well as the concentration of nuclear antigens present in sub- strates...have the same specificity for nuclear an- tigens, the variety of...slide, allows cytoplasmic and nuclear fluorescence to be differentiated...ANA results, the relative cost of reagents and tech- nologist...

C H Casavant; A C Hart; D P Stites

1979-11-01T23:59:59.000Z

418

Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same  

DOE Patents [OSTI]

Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

Tsien, Roger Y. (La Jolla, CA); Wang, Lei (San Diego, CA)

2008-07-01T23:59:59.000Z

419

Red tide detection and tracing using MODIS fluorescence data: A regional example in SW Florida coastal waters  

E-Print Network [OSTI]

Red tide detection and tracing using MODIS fluorescence data: A regional example in SW Florida-time data from the MODIS satellite sensor was used to detect and trace a harmful algal bloom (HAB), or red to interferences of other water constituents. The red tide that formed from November to December 2004 off SW

420

2-dimensional ion velocity distributions measured by laser-induced fluorescence above a radio-frequency biased silicon wafer  

E-Print Network [OSTI]

2-dimensional ion velocity distributions measured by laser-induced fluorescence above a radio-frequency cm diameter, 2.2 MHz-biased silicon wafer in a commercial inductively coupled plasma processing rf frequencies,5­7,18 non-sinusoidal waveforms8 and pulsed power.9,19 Control of IEDs in many

California at Los Angles, University of

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421

Technical description of candidate fluorescence compounds and radioisotopes for a nuclear smuggling deterrence tag (IL500E)  

SciTech Connect (OSTI)

This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials; (2) inventory control of long-term stored items containing special nuclear materials; and (3) tracking materials transferred between facilities. The tagging system uses four types of tagging materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. Currently, 18 long-lived radioisotopes, 38 short-live radioisotopes and 10 fluorescent compounds have been selected as candidate materials for the tagging system.

Hartenstein, S.D.; Aryaeinejad, R.

1996-03-01T23:59:59.000Z

422

Laser-Induced Fluorescence Detection in High-Throughput Screening of Heterogeneous Catalysts and Single Cells Analysis  

SciTech Connect (OSTI)

Laser-induced fluorescence detection is one of the most sensitive detection techniques and it has found enormous applications in various areas. The purpose of this research was to develop detection approaches based on laser-induced fluorescence detection in two different areas, heterogeneous catalysts screening and single cell study. First, we introduced laser-induced imaging (LIFI) as a high-throughput screening technique for heterogeneous catalysts to explore the use of this high-throughput screening technique in discovery and study of various heterogeneous catalyst systems. This scheme is based on the fact that the creation or the destruction of chemical bonds alters the fluorescence properties of suitably designed molecules. By irradiating the region immediately above the catalytic surface with a laser, the fluorescence intensity of a selected product or reactant can be imaged by a charge-coupled device (CCD) camera to follow the catalytic activity as a function of time and space. By screening the catalytic activity of vanadium pentoxide catalysts in oxidation of naphthalene, we demonstrated LIFI has good detection performance and the spatial and temporal resolution needed for high-throughput screening of heterogeneous catalysts. The sample packing density can reach up to 250 x 250 subunits/cm{sub 2} for 40-{micro}m wells. This experimental set-up also can screen solid catalysts via near infrared thermography detection.

Hui Su

2001-05-01T23:59:59.000Z

423

Laser fluorescence study of AIO formed in the reaction AI + O2: Product state distribution, dissociation energy, and radiative  

E-Print Network [OSTI]

.5 ± I kcallmole is recommended for aluminum monoxide. Direct measurement of the fluorescence decay + 0 has been studied by Fontijn, Felder, and Houghton! in a fast- flow reactor. They find, comparison of the ex- perimental and theoretical internal state distributions 1824 The Journal of Chemical

Zare, Richard N.

424

Orange Fluorescent Proteins: Structural Studies of LSSmOrange, PSmOrange and PSmOrange2  

E-Print Network [OSTI]

to greatly decrease the energy of photoconversion and increase its efficiency of photoswitching. Fluorescence Editor: Maria Sola, Molecular Biology Institute of Barcelona, CSIC, Spain Received March 13, 2014 of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic

Verkhusha, Vladislav V.

425

Microarray Immunoassay for Phenoxybenzoic Acid Using Polymer Encapsulated Eu:Gd2O3 Nanoparticles as Fluorescent Labels  

Science Journals Connector (OSTI)

Lanthanide oxides are commonly used as fluorescent materials in the lighting industry. ... PBA is a generic biomarker of human exposure to pyrethroids, a group of highly potent insecticides widely used in agriculture, forestry, horticulture, animal and public health, and households. ... Particle size distributions were measured on a Nanotrac particle size analyzer, model NPA150 from Microtrac Inc. (Montgomeryville, PA), by dynamic light scattering technology. ...

Mikaela Nichkova; Dosi Dosev; Shirley J. Gee; Bruce D. Hammock; Ian M. Kennedy

2005-09-23T23:59:59.000Z

426

Shape-Selective Sorption and Fluorescence Sensing of Aromatics in a Flexible Network of Tetrakis[(4-methylthiophenyl)ethynyl]silane  

E-Print Network [OSTI]

Shape-Selective Sorption and Fluorescence Sensing of Aromatics in a Flexible Network of Tetrakis[(4 entering the pores, enabling, for example, the selective sorption of benzene over hexafluorobenzene networks.1-6 Examples of sorption studies in crystalline coordination networks include selective binding

Li, Jing

427

High energy resolution five-crystal spectrometer for high quality fluorescence and absorption measurements on an X-ray Absorption  

E-Print Network [OSTI]

-ray photons from a large solid angle with five spherically bent crystals. It will cover a large energy rangeHigh energy resolution five-crystal spectrometer for high quality fluorescence and absorption however presents some limitations related to the limited energy resolution and saturation. Crystal

Paris-Sud XI, Université de

428

Replication-Competent Influenza A Virus That Encodes a Split-Green Fluorescent Protein-Tagged PB2 Polymerase Subunit Allows Live-Cell Imaging of the Virus Life Cycle  

Science Journals Connector (OSTI)

...Influenza A Virus That Encodes a Split-Green Fluorescent Protein-Tagged PB2 Polymerase...circumvent this limitation, we used the split-green fluorescent protein (split-GFP) system...complete replacement of a viral protein by green fluorescent protein (GFP) (e.g...

Sergiy V. Avilov; Dorothe Moisy; Sandie Munier; Oliver Schraidt; Nadia Naffakh; Stephen Cusack

2011-11-23T23:59:59.000Z

429

Ultrasound guided fluorescence molecular tomography with improved quantification by an attenuation compensated born-normalization and in vivo preclinical study of cancer  

SciTech Connect (OSTI)

Ultrasound imaging, having the advantages of low-cost and non-invasiveness over MRI and X-ray CT, was reported by several studies as an adequate complement to fluorescence molecular tomography with the perspective of improving localization and quantification of fluorescent molecular targets in vivo. Based on the previous work, an improved dual-modality Fluorescence-Ultrasound imaging system was developed and then validated in imaging study with preclinical tumor model. Ultrasound imaging and a profilometer were used to obtain the anatomical prior information and 3D surface, separately, to precisely extract the tissue boundary on both sides of sample in order to achieve improved fluorescence reconstruction. Furthermore, a pattern-based fluorescence reconstruction on the detection side was incorporated to enable dimensional reduction of the dataset while keeping the useful information for reconstruction. Due to its putative role in the current imaging geometry and the chosen reconstruction technique, we developed an attenuation compensated Born-normalization method to reduce the attenuation effects and cancel off experimental factors when collecting quantitative fluorescence datasets over large area. Results of both simulation and phantom study demonstrated that fluorescent targets could be recovered accurately and quantitatively using this reconstruction mechanism. Finally, in vivo experiment confirms that the imaging system associated with the proposed image reconstruction approach was able to extract both functional and anatomical information, thereby improving quantification and localization of molecular targets.

Li, Baoqiang; Berti, Romain; Abran, Maxime; Lesage, Frdric, E-mail: frederic.lesage@polymtl.ca [Institute of Biomedical Engineering, cole Polytechnique de Montral, Montreal, Quebec H3C 3A7 (Canada) [Institute of Biomedical Engineering, cole Polytechnique de Montral, Montreal, Quebec H3C 3A7 (Canada); Montreal Heart Institute, Montreal, Quebec H1T 1C8 (Canada)

2014-05-15T23:59:59.000Z

430

The Interaction between Rac1 and Its Guanine Nucleotide Dissociation Inhibitor (GDI), Monitored by a Single Fluorescent Coumarin Attached to GDI  

Science Journals Connector (OSTI)

The Interaction between Rac1 and Its Guanine Nucleotide Dissociation Inhibitor (GDI), Monitored by a Single Fluorescent Coumarin Attached to GDI ... The interaction of rac with guanine nucleotide dissociation inhibitor protein (rhoGDI) is described, using GDI fluorescently labeled on its single cysteine with N-[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide (MDCC). ... The labeled GDI shows a 70% decrease in fluorescence emission on binding geranylgeranylated rac1GDP and has an affinity for rac1 within a factor of 2 of the unlabeled GDI. ...

Anthony R. Newcombe; Richard W. Stockley; Jackie L. Hunter; Martin R. Webb

1999-05-04T23:59:59.000Z

431

A fluorescent screening platform for the rapid evaluation of chemicals in cellular reprogramming  

Science Journals Connector (OSTI)

Current strategies to monitor reprogramming into induced pluripotent stem cells (iPSCs) are limited in that they rely on the recognition of advanced stage biomarkers or they involve the transduction of genetically-modified cells. These limitations are particularly problematic in high-throughput screenings where cell availability, low cost and a rapid experimental protocol are critical issues. Herein we report the application of a pluripotent stem cell fluorescent probe (i.e. CDy1) as a reporter for the rapid screening of chemicals in reprogramming iPSCs. \\{CDy1\\} stains early-stage iPSCs at 7dpi as well as matured iPSCs; hence it can partially overcome the slow kinetics of the reprogramming process. As a proof of concept, we employed a CDy1-based screening in 384 well-plates to examine the effect of newly synthesized hydroxamic acid derivatives in reprogramming mouse fibroblasts transduced with Oct4, Sox2 and Klf-4 without c-Myc. One compound (126) was identified as a reprogramming enhancer by 2.5-fold and we confirmed that 126 behaves as a histone deacetylase (HDAC) inhibitor. The successful identification of novel small molecules enhancing the generation of iPSCs by means of a rapid and simple protocol demonstrates the suitability of this CDy1-based screening platform for the large scale and high-throughput evaluation of iPSC modulators.

Marc Vendrell; Sung-Jin Park; Yogeswari Chandran; Chi-Lik Ken Lee; Hyung-Ho Ha; Nam-Young Kang; Seong-Wook Yun; Young-Tae Chang

2012-01-01T23:59:59.000Z

432

Interfacing picoliter droplet microfluidics with addressable microliter compartments using fluorescence activated cell sorting  

Science Journals Connector (OSTI)

Abstract Droplet microfluidic platforms have, while enabling high-throughput manipulations and the assaying of single cell scale compartments, been lacking interfacing to allow macro scale access to the output from droplet microfluidic operations. Here, we present a simple and high-throughput method for individually directing cell containing droplets to an addressable and macro scale accessible microwell slide for downstream analysis. Picoliter aqueous droplets containing low gelling point agarose and eGFP expressing Escherichia coli (E. coli) are created in a microfluidic device, solidified to agarose beads and transferred into an aqueous buffer. A Fluorescence activated cell sorter (FACS) is used to sort agarose beads containing cells into microwells in which the growth and expansion of cell colonies is monitored. We demonstrate fast sorting and high accuracy positioning of sorted 15?m gelled droplet agarose beads into microwells (14נ48) on a 25mmנ75mm microscope slide format using a FACS with a 100?m nozzle and an xy-stage. The interfacing method presented here enables the products of high-throughput or single cell scale droplet microfluidics assays to be output to a wide range of microtiter plate formats familiar to biological researchers lowering the barriers for utilization of these microfluidic platforms.

Yunpeng Bai; Emilie Weibull; Haakan N. Joensson; Helene Andersson-Svahn

2014-01-01T23:59:59.000Z

433

Isotope shifts of natural Sr+ measured by laser fluorescence in a sympathetically cooled Coulomb crystal  

E-Print Network [OSTI]

We measured by laser spectroscopy the isotope shifts between naturally-occurring even-isotopes of strontium ions for both the $5s\\,\\,^2S_{1/2}\\to 5p\\,\\,^2P_{1/2}$ (violet) and the $4d\\,\\,^2D_{3/2}\\to 5p\\,\\,^2P_{1/2}$ (infrared) dipole-allowed optical transitions. Fluorescence spectra were taken by simultaneous measurements on a two-component Coulomb crystal in a linear Paul trap containing $10^3$--$10^4$ laser-cooled Sr$^+$ ions. The isotope shifts are extracted from the experimental spectra by fitting the data with the analytical solution of the optical Bloch equations describing a three-level atom in interaction with two laser beams. This technique allowed us to increase the precision with respect to previously reported data obtained by optogalvanic spectroscopy or fast atomic-beam techniques. The results for the $5s\\,\\,^2S_{1/2}\\to 5p\\,\\,^2P_{1/2}$ transition are $\

Brice Dubost; Romain Dubessy; Benjamin Szymanski; Samuel Guibal; Jean-Pierre Likforman; Luca Guidoni

2014-02-14T23:59:59.000Z

434

Fluorescence analysis can identify movable oil in self-sourcing reservoirs  

SciTech Connect (OSTI)

The recent surge of activity involving self-sourcing reservoirs and horizontal drilling recognizes a little tapped niche in the domestic energy mix. Such prolific pays as the Cretaceous Bakken and Austin Chalk have drawn research interest and large amounts of investment capital. Fluorescence analysis can discern movable oil--as opposed to exhausted source rock--in such reservoirs with an inexpensive test. Other potential targets are the Cretaceous Mesaverde in the Piceance basin, Devonian New Albany shale in Kentucky, Devonian Antrim shale in the Michigan basin, and the Cretaceous Niobrara, Mancos, and Pierre formations in Colorado and New Mexico. To insure success in this niche this key question must be answered positively: Is movable oil present in the reservoir? Even if tectonic studies verify a system of open fractures, sonic logs confirm overpressuring in the zone, and resistivity logs document the maturity of the source, the ultimate question remains: Is movable oil in the fractures available to flow to the borehole? The paper explains a technique that will answer these questions.

Calhoun, G.G. [Calhoun (Gerry G.), Midland, TX (United States)

1995-06-05T23:59:59.000Z

435

Comparison of NO and OH planar fluorescence temperature measurements in scramjet model flowfields  

SciTech Connect (OSTI)

The use of nitric oxide (NO) and the hydroxyl radical (OH) as temperature tracers, in a two-line planar laser-induced fluorescence technique, is examined in the context of a supersonic mixing and combustion flowfield. The temperature measurements were based on the sequential excitation of two transitions, either in the A implied by X (0,0) band of NO near 226 nm or the A implied by X (1,0) band of OH near 283 nm. The measurements were obtained for each species through the use of two lasers and two cameras, with each camera integrating signal induced from only one of the lasers. Both temporally resolved and frame-averaged temperature measurements of each species are presented. Additional results include simultaneous NO and OH visualizations, in which seeded NO marks the fuel jet fluid and nascent OH marks the reaction zones and convected combustion gases. A detailed temperature comparison shows good agreement in the common measurement regions and indicates that shot noise is the largest source of uncertainty. The comparison also illustrates the importance of a careful interpretation of the measurements, since, depending on the origin of the tracer and the degree of mixing, the measurements may be biased toward the fuel, freestream, or reaction zone temperatures. 33 refs.

Mcmillin, B.K.; Seitzman, J.M.; Hanson, R.K. [Natl. Inst. of Standards and Technology, Gaithersburg, MD (United States)

1994-10-01T23:59:59.000Z

436

Laser-induced fluorescence velocity measurements of a diverging cusped-field thruster  

Science Journals Connector (OSTI)

Measurements are presented of the most probable time-averaged ion velocities within the acceleration channel and in the plume of a diverging cusped-field thruster operating on xenon. Xenon ion velocities for the thruster are derived from laser-induced fluorescence measurements of the 5d[4]7/26p[3]5/2 xenon ion excited state transition centred at ? = 834.72?nm. The thruster is operated in both a high-current mode, where the anode discharge current is shown to oscillate periodically, and a low-current mode where operation is relatively quiescent. In the low-current mode, ion emission is predominantly in the form of a conical jet, whereas in the high-current mode, the emission is still divergent but more diffuse throughout the cone angle. These time-average measurements provide insight into the structure of the acceleration region. However, discerning the mechanism for the diffuse ion emission in the strongly oscillating high-current mode will require ion velocity measurements capable of resolving the time-dependent behaviour of the discharge.

N A MacDonald; M A Cappelli; S R Gildea; M Martnez-Snchez; W A Hargus Jr

2011-01-01T23:59:59.000Z

437

Determination of polynuclear aromatic hydrocarbons in seafood by liquid chromatography with fluorescence detection  

SciTech Connect (OSTI)

Modification of a previously published method for determination of polynuclear aromatic hydrocarbons (PAHs) produces very clean seafood extracts in less than half the time. After alkaline digestion of the seafood, PAHs were partitioned into 1,2,3-trichlorotrifluoroethane. The resulting extract was cleaned up by solid-phase extraction on alumina, silica, and C{sub 18} adsorbents and then analyzed by gradient reversed-phase liquid chromatography with programmable fluorescence detection. Average recoveries of 12 PAHs [acenaphthene, anthracene, fluoranthene, pyrene, benz(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)-fluoranthene, benzo(k)-fluoranthene, benzo(a)pyrene, dibenz(a,h)anthracene, benzo(ghi)perylene, and indeno(1,2,3-cd)pyrene] from 5 different matrixes (mussels, oysters, clams, crabmeat, and salmon)spiked at low parts-per-billion levels ranged from 76 to 94%. Estimated limits of quantitation ranged from 0.01 to 0.6 ppb PAHs in extracts that were free of matrix interferences. Results of analyses of a mussels standard reference material obtained from the National Institute of Standards and Technology were in good agreement with the certified values. 16 refs., 3 figs., 4 tabs.

Perfetti, G.A.; Nyman, P.J.; Fisher, S.; Joe, F.L. Jr.; Diachenko, G.W. [Food and Drug Administration, Washington, DC (United States)

1992-09-01T23:59:59.000Z

438

X-ray optics for scanning fluorescence microscopy and other applications  

SciTech Connect (OSTI)

Scanning x-ray fluorescence microscopy is analogous to scanning electron microscopy. Maps of chemical element distribution are produced by scanning with a very small x-ray beam. Goal is to perform such scanning microscopy with resolution in the range of <1 to 10 {mu}m, using standard laboratory x-ray tubes. We are investigating mirror optics in the Kirkpatrick-Baez (K-B) configuration. K-B optics uses two curved mirrors mounted orthogonally along the optical axis. The first mirror provides vertical focus, the second mirror provides horizontal focus. We have used two types of mirrors: synthetic multilayers and crystals. Multilayer mirrors are used with lower energy radiation such as Cu K{alpha}. At higher energies such as Ag K{alpha}, silicon wafers are used in order to increase the incidence angles and thereby the photon collection efficiency. In order to increase the surface area of multilayers which reflects x-rays at the Bragg angle, we have designed mirrors with the spacing between layers graded along the optic axis in order to compensate for the changing angle of incidence. Likewise, to achieve a large reflecting surface with silicon, the wafers are placed on a specially designed lever arm which is bent into a log spiral by applying force at one end. In this way, the same diffracting angle is maintained over the entire surface of the wafer, providing a large solid angle for photon collection.

Ryon, R.W. [Lawrence Livermore National Lab., CA (United States); Warburton, W.K. [X-Ray Instrumentation Associates, Menlo Park, CA (United States)

1992-05-01T23:59:59.000Z

439

3D Fluorescent and Reflective Imaging of Whole Stardust Tracks in Aerogel  

SciTech Connect (OSTI)

The NASA Stardust mission returned to earth in 2006 with the cometary collector having captured over 1,000 particles in an aerogel medium at a relative velocity of 6.1 km/s. Particles captured in aerogel were heated, disaggregated and dispersed along 'tracks' or cavities in aerogel, singular tracks representing a history of one capture event. It has been our focus to chemically and morphologically characterize whole tracks in 3-dimensions, utilizing solely non-destructive methods. To this end, we have used a variety of methods: 3D Laser Scanning Confocal Microscopy (LSCM), synchrotron X-ray fluorescence (SXRF), and synchrotron X-ray diffraction (SXRD). In the past months we have developed two new techniques to aid in data collection. (1) We have received a new confocal microscope which has enabled autofluorescent and spectral imaging of aerogel samples. (2) We have developed a stereo-SXRF technique to chemically identify large grains in SXRF maps in 3-space. The addition of both of these methods to our analytic abilities provides a greater understanding of the mechanisms and results of track formation.

Greenberg, M.; Ebel, D.S. (AMNH)

2011-11-07T23:59:59.000Z

440

Single and two-photon fluorescence control of Er{sup 3+} ions by phase-shaped femtosecond laser pulse  

SciTech Connect (OSTI)

We experimentally demonstrate the control of the single and two-photon fluorescence (SPF and TPF) in Er{sup 3+} ions by shaping the femtosecond laser pulse with a ? or square phase modulation. With the low laser intensity (8.4??10{sup 10}?W/cm{sup 2}), SPF keeps a constant while TPF is effectively suppressed by the two control schemes. With the high laser intensity (1.2??10{sup 13}?W/cm{sup 2}), both SPF and TPF are simultaneously enhanced or suppressed by the ? phase modulation, and SPF is enhanced while TPF is effectively suppressed by the square phase modulation. The up/down-conversion fluorescence enhancement, suppression, or tuning by the optical control method can greatly expand its applications in various related fields.

Zhang, Shian, E-mail: sazhang@phy.ecnu.edu.cn; Ding, Jingxin; Lu, Chenhui; Jia, Tianqing; Sun, Zhenrong, E-mail: zrsun@phy.ecnu.edu.cn [State Key Laboratory of Precision Spectroscopy, and Department of Physics, East China Normal University, Shanghai 200062 (China)] [State Key Laboratory of Precision Spectroscopy, and Department of Physics, East China Normal University, Shanghai 200062 (China); Xu, Shuwu [State Key Laboratory of Precision Spectroscopy, and Department of Physics, East China Normal University, Shanghai 200062 (China) [State Key Laboratory of Precision Spectroscopy, and Department of Physics, East China Normal University, Shanghai 200062 (China); School of Science, Nantong University, Nantong 226007 (China); Qiu, Jianrong [State Key Laboratory of Luminescent Materials and Devices, and Institute of Optical Communication Materials, South China University of Technology, Wushan Road 381, Guangzhou 510640 (China)] [State Key Laboratory of Luminescent Materials and Devices, and Institute of Optical Communication Materials, South China University of Technology, Wushan Road 381, Guangzhou 510640 (China)

2014-01-06T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

On the Search for Nuclear Resonance Fluorescence Signatures of 235U and 238U above 3 MeV  

SciTech Connect (OSTI)

Nuclear resonance fluorescence is a physical process that provides an isotope-specific signature that could be used for the identification and characterization of materials. The technique involves the detection of prompt discrete-energy photons emitted from a sample that is exposed to MeV-energy photons. Potential applications of the technique range from detection of high explosives to characterization of special nuclear materials such as 235U. Pacific Northwest National Laboratory and Passport Systems have collaborated to conduct a pair of measurements to search for a nuclear resonance fluorescence response of 235U above 3 MeV and of 238U above 5 MeV using an 8 g sample of highly enriched uranium and a 90 g sample of depleted uranium. No new signatures were observed. The minimum detectable integrated cross section for 235U is presented.

Warren, Glen A.; Caggiano, Joseph A.; Bertozzi, William; Korbly, Steve; Ledoux, Robert; Park, William H.

2010-02-01T23:59:59.000Z

442

Quantitative analysis of phosphosilicate glass films on silicon wafers for calibration of x-ray fluorescence spectrometry standards  

SciTech Connect (OSTI)

The phosphorus and silicon contents of phosphosilicate glass films deposited by chemical vapor deposition (CVD) on silicon wafers were determined. These films were prepared for use as x-ray fluorescence (XRF) spectrometry standards. The thin films were removed from the wafer by etching with dilute hydrofluoric acid, and the P and Si concentrations in solution were determined by inductively coupled plasma atomic emission spectroscopy (ICP). The calculated phosphorus concentration ranged from 2.2 to 12 wt %, with an uncertainty of 2.73 to 10.1 relative percent. Variation between the calculated weight loss (summation of P/sub 2/O/sub 5/ and SiO/sub 2/ amounts as determined by ICP) and the measured weight loss (determined gravimetrically) averaged 4.9%. Results from the ICP method, Fourier transform-infrared spectroscopy (FT-IR), dispersive infrared spectroscopy, electron microprobe, and x-ray fluorescence spectroscopy for the same samples are compared.

Weissman, S.H.

1983-01-01T23:59:59.000Z

443

Modeling green fluorescent protein transcription, translation and modification as a method to obtain NF-kappaB activation profiles  

E-Print Network [OSTI]

Jayaraman Committee Members, Juergen Hahn Mike McShane Head of Department, N.K. Anand August 2007 Major Subject: Chemical Engineering iii ABSTRACT Modeling Green Fluorescent Protein Transcription, Translation and Modification as a Method... my profound thanks to my advisor, Dr. Arul Jayaraman, and my committee members, Dr. Juergen Hahn and Dr. Mike McShane, for their guidance and support throughout the course of this research. I would like to extend my gratitude to Jacky Huang who...

Laible, Allyson Marie

2009-05-15T23:59:59.000Z

444

Kinetics of Cdc42 Membrane Extraction by Rho-GDI Monitored by Real-Time Fluorescence Resonance Energy Transfer  

Science Journals Connector (OSTI)

Kinetics of Cdc42 Membrane Extraction by Rho-GDI Monitored by Real-Time Fluorescence Resonance Energy Transfer ... This assay enabled us to directly monitor the (GDI-induced) release of Cdc42 from membranes. ... Specifically, we propose that the GDI first binds rapidly to membrane-associated Cdc42 and then a slower isomerization occurs which represents the rate-limiting step for the dissociation of the Cdc42?RhoGDI complex from membranes. ...

Tyzoon K. Nomanbhoy; Jon W. Erickson; Richard A. Cerione

1999-01-16T23:59:59.000Z

445

COMPARISON OF DETECTION CAPABILITY FOR ACOUSTIC THERMOGRAPHY, VISUAL INSPECTION AND FLUORESCENT PENETRANT INSPECTION ON GAS TURBINE COMPONENTS  

Science Journals Connector (OSTI)

The innovative NDE inspection system Acoustic Thermography is developed with Sonic Infrared (Sonic IR) technology. Since the probability of detection is sensitive to the flaw characteristics the fabricated flaws could not simulated the nature flaws with accuracy. The study is focus on gas turbine blades with service induced fatigue cracks. The detection capability of this innovative NDE inspection system is compared with two traditional NDE methods: Visual Inspection and Fluorescent Penetrant Inspection. POD curves for each technique were generated and compared.

Y. Guo; F. R. Ruhge

2009-01-01T23:59:59.000Z

446

Characterization of atmospheric aerosols using Synchroton radiation total reflection X-ray fluorescence and Fe K-edge total reflection X-ray fluorescence-X-ray absorption near-edge structure  

Science Journals Connector (OSTI)

In this study a new procedure using Synchrotron total reflection X-ray fluorescence (SR-TXRF) to characterize elemental amounts in atmospheric aerosols down to particle sizes of 0.015 um is presented. The procedure was thoroughly evaluated regarding bounce off effects and blank values. Additionally the potential of total reflection X-ray fluorescenceX-ray absorption near edge structure (SR-TXRF-XANES) for speciation of FeII/III down to amounts of 34pg in aerosols which were collected for 1h is shown. The aerosols were collected in the city of Hamburg with a low pressure Berner impactor on Si carriers covered with silicone over time periods of 60 and 20min each. The particles were collected in four and ten size fractions of 10.08.0?m, 8.02.0?m, 2.00.13?m 0.130.015?m (aerodynamic particle size) and 1530nm, 3060nm, 60130nm, 130250nm, 250500nm, 0.51?m, 12?m, 24?m, 48?m, 816?m. Prior to the sampling bounce off effects on Silicone and Vaseline coated Si carriers were studied with total reflection X-ray fluorescence. According to the results silicone coated carriers were chosen for the analysis. Additionally, blank levels originating from the sampling device and the calibration procedure were studied. Blank levels of Fe corresponded to 110% of Fe in the aerosol samples. Blank levels stemming from the internal standard were found to be negligible. The results from the Synchroton radiation total reflection X-ray fluorescence analysis of the aerosols showed that 20min of sampling time gave still enough sample material for elemental determination of most elements. For the determination of the oxidation state of Fe in the aerosols different Fe salts were prepared as a reference from suspensions in isopropanol. The results from the Fe K-edge Synchroton radiation total reflection X-ray fluorescence-X-ray absorption near-edge structure analysis of the aerosol samples showed that mainly Fe(III) was present in all particle size fractions.

U.E.A. Fittschen; F. Meirer; C. Streli; P. Wobrauschek; J. Thiele; G. Falkenberg; G. Pepponi

2008-01-01T23:59:59.000Z

447

Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe  

E-Print Network [OSTI]

We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide ...

McQuade, Lindsey E.

448

Light-induced polarization effects in atoms with partially resolved hyperfine structure and applications to absorption, fluorescence, and nonlinear magneto-optical rotation  

E-Print Network [OSTI]

Light-induced polarization effects in atoms with partially resolved hyperfine structure and applications to absorption, fluorescence, and nonlinear magneto-optical rotation M. Auzinsh* Department 9 November 2009 The creation and detection of atomic polarization is examined theoretically through

Auzinsh, Marcis

449

Detection limits of high temperature superconducting materials on various substrates by energy dispersive X-ray fluorescence and proton induced X-ray emission methods  

Science Journals Connector (OSTI)

Application of energy dispersive X-ray fluorescence (EDXRF) and proton induced X-ray emission (PIXE) methods has been demonstrated for determining the elemental composition of thin film superconducting materia...

M Lal; H N Bajpai; D Joseph; R K Choudhury

1990-04-01T23:59:59.000Z

450

A matter of collection and detection for intraoperative and noninvasive near-infrared fluorescence molecular imaging: To see or not to see?  

SciTech Connect (OSTI)

Purpose: Although fluorescence molecular imaging is rapidly evolving as a new combinational drug/device technology platform for molecularly guided surgery and noninvasive imaging, there remains no performance standards for efficient translation of first-in-humans fluorescent imaging agents using these devices. Methods: The authors employed a stable, solid phantom designed to exaggerate the confounding effects of tissue light scattering and to mimic low concentrations (nMpM) of near-infrared fluorescent dyes expected clinically for molecular imaging in order to evaluate and compare the commonly used charge coupled device (CCD) camera systems employed in preclinical studies and in human investigational studies. Results: The results show that intensified CCD systems offer greater contrast with larger signal-to-noise ratios in comparison to their unintensified CCD systems operated at clinically reasonable, subsecond acquisition times. Conclusions: Camera imaging performance could impact the success of future first-in-humans near-infrared fluorescence imaging agent studies.

Zhu, Banghe; Rasmussen, John C.; Sevick-Muraca, Eva M., E-mail: Eva.Sevick@uth.tmc.edu [Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center, Houston, Texas 77030 (United States)

2014-02-15T23:59:59.000Z

451

Squaraine-Derived Rotaxanes: Sterically Protected Fluorescent Near-IR Dyes Easwaran Arunkumar, Christopher C. Forbes, Bruce C. Noll, and Bradley D. Smith*  

E-Print Network [OSTI]

, Christopher C. Forbes, Bruce C. Noll, and Bradley D. Smith* Department of Chemistry and Biochemistry, UniVersity of Notre Dame, Notre Dame, Indiana 46556 Received December 17, 2004; E-mail: smith.115@nd.edu Fluorescent

Smith, Bradley D.

452

Improved Fluorescent In Situ Hybridization Method for Detection of Bacteria from Activated Sludge and River Water by Using DNA Molecular Beacons and Flow Cytometry  

Science Journals Connector (OSTI)

...higher discriminatory power of molecular beacons over...better discriminatory power (using signal-laser...better discriminatory power than that of standard...compositions of lakes and oceans: a first comparison based on fluorescence...

Jeremy Lenaerts; Hilary M. Lappin-Scott; Jonathan Porter

2007-02-02T23:59:59.000Z

453

Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore  

SciTech Connect (OSTI)

The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, {lambda}{sub ex} = 552 nm, {lambda}{sub em} = 578 nm) and its bright far-red fluorescent variant Katushka ({lambda}{sub ex} = 588 nm, {lambda}{sub em} = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 {angstrom} at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f.

Pletneva, Nadya V.; Pletnev, Vladimir Z.; Shemiakina, Irina I.; Chudakov, Dmitriy M.; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei (Russ. Acad. Sci.); (SAIC); (NCI)

2012-08-10T23:59:59.000Z

454

THE SAP3 COMPUTER PROGRAM FOR QUANTITATIVE MULTIELEMENT ANALYSIS BY ENERGY DISPERSIVE X-RAY FLUORESCENCE  

SciTech Connect (OSTI)

SAP3 is a dual-function FORTRAN computer program which performs peak analysis of energy-dispersive x-ray fluorescence spectra and then quantitatively interprets the results of the multielement analysis. It was written for mono- or bi-chromatic excitation as from an isotopic or secondary excitation source, and uses the separate incoherent and coherent backscatter intensities to define the bulk sample matrix composition. This composition is used in performing fundamental-parameter matrix corrections for self-absorption, enhancement, and particle-size effects, obviating the need for specific calibrations for a given sample matrix. The generalized calibration is based on a set of thin-film sensitivities, which are stored in a library disk file and used for all sample matrices and thicknesses. Peak overlap factors are also determined from the thin-film standards, and are stored in the library for calculating peak overlap corrections. A detailed description is given of the algorithms and program logic, and the program listing and flow charts are also provided. An auxiliary program, SPCAL, is also given for use in calibrating the backscatter intensities. SAP3 provides numerous analysis options via seventeen control switches which give flexibility in performing the calculations best suited to the sample and the user needs. User input may be limited to the name of the library, the analysis livetime, and the spectrum filename and location. Output includes all peak analysis information, matrix correction factors, and element concentrations, uncertainties and detection limits. Twenty-four elements are typically determined from a 1024-channel spectrum in one-to-two minutes using a PDP-11/34 computer operating under RSX-11M.

Nielson,, K. K.; Sanders,, R. W.

1982-04-01T23:59:59.000Z

455

Laser-saturated fluorescence of nitric oxide and chemiluminescence measurements in premixed ethanol flames  

SciTech Connect (OSTI)

In this study, nitric oxide laser-saturated fluorescence (LSF) measurements were acquired from premixed ethanol flames at atmospheric pressure in a burner. NO-LSF experimental profiles for fuel-rich premixed ethanol flames ({phi} = 1.34 and {phi} = 1.66) were determined through the excitation/detection scheme of the Q{sub 2}(26.5) rotational line in the A{sup 2}{sigma}{sup +} - X{sup 2}{pi} (0,0) vibronic band and {gamma}(0,1) emission band. A calibration procedure by NO doping into the flame was applied to establish the NO concentration profiles in these flames. Chemiluminescent emission measurements in the (0, 0) vibronic emission bands of the OH{sup *} (A{sup 2}{sigma}{sup +} - X{sup 2}{pi}) and CH{sup *}(A{sup 2}{delta} - X{sup 2}{pi}) radicals were also obtained with high spatial and spectral resolution for fuel-rich premixed ethanol flames to correlate them with NO concentrations. Experimental chemiluminescence profiles and the ratios of the integrated areas under emission spectra (A{sub CH*}/A{sub CH*}(max.) and A{sub CH*}/A{sub OH*}) were determined. The relationships between chemiluminescence and NO concentrations were established along the premixed ethanol flames. There was a strong connection between CH{sup *} radical chemiluminescence and NO formation and the prompt-NO was identified as the governing mechanism for NO production. The results suggest the optimum ratio of the chemiluminescence of two radicals (A{sub CH*}/A{sub OH*}) for NO diagnostic purposes. (author)

Marques, Carla S.T.; Barreta, Luiz G.; Sbampato, Maria E.; dos Santos, Alberto M. [Aerothermodynamic and Hypersonic Division, Institute of Advanced Studies - General Command of Aerospatial Technology, Rodovia dos Tamoios, km 5.5, 12228-001 Sao Jose dos Campos - SP (Brazil)

2010-11-15T23:59:59.000Z

456

Hard X-ray Fluorescence Measurements of Heteroepitaxial Solid Oxide Fuel Cell Cathode Materials  

SciTech Connect (OSTI)

Commonly, SOFCs are operated at high temperatures (above 800C). At these temperatures expensive housing is needed to contain an operating stack as well as coatings to contain the oxidation of the metallic interconnects. Lowering the temperature of an operating device would allow for more conventional materials to be used, thus lowering overall cost. Understanding the surface chemical states of cations in the surface of the SOFC cathode is vital to designing a system that will perform well at lower temperatures. The samples studied were grown by pulsed laser deposition (PLD) at the Environmental Molecular Sciences Laboratory (EMSL) at Pacific Northwest National Laboratory (PNNL). 20% strontium doped lanthanum manganite (LSM-20) was grown on YSZ and NGO (neodymium gallate). The films on YSZ have a fiber texture. LSM-20 on NGO is heteroepitaxial. Lanthanum strontium cobalt ferrite (LSCF-6428) films were grown on LAO and YSZ with a GDC barrier layer. Total X-ray Reflection Fluorescence (TXRF) was used to depth profile the samples. In a typical experiment, the angle of the incident beam is varied though the critical angle. Below the critical angle, the x-ray decays as an evanescent wave and will only penetrate the top few nanometers. TXRF experiments done on LSM films have suggested strontium segregates to the surface and form strontium enriched nanoparticles (1). It should be pointed out that past studies have focused on 30% strontium A-site doping, but this project uses 20% strontium doped lanthanum manganite. XANES and EXAFS data were taken as a function of incoming angle to probe composition as a function of depth. XANES spectra can be difficult to analyze fully. For other materials density functional theory calculations compared to near edge measurements have been a good way to understand the 3d valence electrons (2).

Davis, Jacob N.; Miara, Lincoln J.; Saraf, Laxmikant V.; Kaspar, Tiffany C.; Gopalan, Srikanth; Pal, Uday B.; Woicik, Joseph C.; Basu, Soumendra N.; Ludwig, Karl F.

2012-12-01T23:59:59.000Z

457

Thickness measurement of aluminum, titanium, titanium silicide, and tungsten silicide films by x-ray fluorescence  

SciTech Connect (OSTI)

X-ray fluorescence (XRF) has received a great deal of attention in the last few years as a quantitative means of determining both the stoichiometry and thickness of many different kinds of films. Examples include Ag and Cu films on mica substrates, Sn-Pb alloys on steel substrates, Al-Ti multilayers on Cu substrates and oxide films on silicon substrates. In XRF the sample is irradiated with x-rays which in turn cause x-rays to be given off by the sample. These x-rays that are given off by the sample can then be analyzed both for energy and intensity. The energy of the x-rays is characteristic of the elemental makeup of the sample and the intensity of the x-rays is dependent on how much of the particular element is present. The intensity then can be related to the thickness of a film if the stoichiometry of the film can be assumed constant. With the increased interest in silicides and more recently, the self-aligned silicide (salicide) process (8-10) for VLSI applications, in-line process monitoring of silicide film thickness has become important to integrated circuit manufacturing. In this study, the number of x-ray photons given of by Al, Ti, titanium silicide, and tungsten silicide films on silicon-based substrates was quantified so that a film thickness for an unknown sample could then be determined easily. In addition, XRF is a more accurate technique, limited principally by the accuracy technique, limited principally by the accuracy of the reference used and the amount of time the x-ray photons are counted.

Ernst, S.; Lee, C.O.; Lee, J.J. (Motorola, Inc., Advanced Products Research and Development Lab., Austin, TX (US))

1988-08-01T23:59:59.000Z

458

Two photon absorption laser induced fluorescence measurements of neutral density in a helicon plasma  

SciTech Connect (OSTI)

We have developed a new diagnostic based on two-photon absorption laser induced fluorescence (TALIF). We use a high intensity (5?MW/cm{sup 2}), narrow bandwidth (0.1?cm{sup ?1}) laser to probe the ground state of neutral hydrogen, deuterium and krypton with spatial resolution better than 0.2?cm, a time resolution of 10?ns, and a measurement cadence of 20?Hz. Here, we describe proof-of-principle measurements in a helicon plasma source that demonstrate the TALIF diagnostic is capable of measuring neutral densities spanning four orders of magnitude; comparable to the edge neutral gradients predicted in the DIII-D tokamak pedestal. The measurements are performed in hydrogen and deuterium plasmas and absolute calibration is accomplished through TALIF measurements in neutral krypton. The optical configuration employed is confocal, i.e., both light injection and collection are accomplished with a single lens through a single optical port in the vacuum vessel. The wavelength resolution of the diagnostic is sufficient to separate hydrogen and deuterium spectra and we present measurements from mixed hydrogen and deuterium plasmas that demonstrate isotopic abundance measurements are feasible. Time resolved measurements also allow us to explore the evolution of the neutral hydrogen density and temperature and effects of wall recycling. We find that the atomic neutral density grows rapidly at the initiation of the discharge, reaching the steady-state value within 1?ms. Additionally, we find that neutral hydrogen atoms are born with 0.08?eV temperatures, not 2?eV as is typically assumed.

Galante, M. E.; Magee, R. M.; Scime, E. E. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States)] [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States)

2014-05-15T23:59:59.000Z

459

Monochromatic wavelength dispersive x-ray fluorescence providing sensitive and selective detection of uranium  

SciTech Connect (OSTI)

Monochromatic wavelength dispersive X-ray fluorescence (MWDXRF) is a sensitive and selective method for elemental compositional analyses. The basis for this instrumental advance is the doubly curved crystal (DCC) optic. Previous work has demonstrated the feasibility of sensitive trace element detection for yttrium as a surrogate for curium in aqueous solutions. Additional measurements have demonstrated similar sensitivity in several different matrix environments which attests to the selectivity of the DCC optic as well as the capabilities of the MWDXRF concept. The objective of this effort is to develop an improved Pu characterization method for nuclear fuel reprocessing plants. The MWDXRF prototype instrument is the second step in a multi-year effort to achieve an improved Pu assay. This work will describe a prototype MWDXRF instrument designed for uranium detection and characterization. The prototype consists of an X-ray tube with a rhodium anode and a DCC excitation optic incorporated into the source. The DCC optic passes the RhK{alpha} line at 20.214 keV for monochromatic excitation of the sample. The source is capable of 50 W power at 50 kV and 1.0 mA operation. The x-ray emission from the sample is collected by a DCC optic set at the UL{alpha} line of 13.613 keV. The collection optic transmits the UL{alpha} x-rays to the silicon drift detector. The x-ray source, sample, collection optic and detector are all mounted on motion controlled stages for the critical alignment of these components. The sensitivity and selectivity of the instrument is obtained through the monochromatic excitation and the monochromatic detection. The prototype instrument performance has a demonstrated for sensitivity for uranium detection of around 2 ppm at the current state of development. Further improvement in sensitivity is expected with more detailed alignment.

Havrilla, George J [Los Alamos National Laboratory; Collins, Michael L [Los Alamos National Laboratory; Montoya, Velma M [Los Alamos National Laboratory; Chen, Zewu [XOS; Wei, Fuzhong [XOS

2010-01-01T23:59:59.000Z

460

A new anthropometric phantom for calibrating in vivo measurements of stable lead in the human leg using X-ray fluorescence  

SciTech Connect (OSTI)

A new anthropometric phantom has been developed for calibrating in vivo measurements of stable lead deposited in bone using x-ray fluorescence. The phantom reproduces the shape of the mid shaft of the adult human leg and is fabricated using polyurethanes and calcium carbonate to produce materials that exhibit the same density, energy transmission, and calcium content as cortical bone, bone marrow, and muscle. The phantom includes a removable tibia fabricated using simulants for cortical bone and bone marrow to which a precise amount of stable lead has been added to cortical bone. The formulations used in fabricating the new anthropometric phantom are much more uniform in density and composition than the conventional phantom made from Plexiglas cylinders filled with plaster-of-Paris. The energy spectrum from an x-ray fluorescence measurement of the phantom using a {sup 109}Cd source is indistinguishable from an in vivo x-ray fluorescence measurement of the human leg, demonstrating that the materials used in the phantom exhibit the same radiological properties as human tissue. Likewise, results from x-ray fluorescence measurements of the phantom exhibit the same positional dependency as the human leg and vary by approximately 36% when, for example, the phantom containing 54 ppm of stable lead in the tibia was rotated by only 15 degrees. The detection limit for a 30 min {sup 109}Cd K shell x-ray fluorescence in vivo measurement is approximately 20 ppm determined from a background measurement using the new phantom containing no added lead in the muscle, bone, or bone marrow. The new anthropometric phantom significantly improves in vivo x-ray fluorescence calibration measurements by (1) faithfully reproducing the anatomy of the human leg, (2) having components that exhibit radiological properties similar to that of human tissue, and (3) providing a realistic calibration standard that can be used for in vivo x-ray fluorescence intercomparison measurements.

Spitz, H.; Jenkins, M.; Lodwick, J.; Bornschein, R.

2000-02-01T23:59:59.000Z

Note: This page contains sample records for the topic "fluorescent compact fluorescent" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

X-RAY FLUORESCENCE ANALYSIS OF HANFORD LOW ACTIVITY WASTE SIMULANTS  

SciTech Connect (OSTI)

Savannah River National Laboratory (SRNL) was requested to develop an x-ray fluorescence (XRF) spectrometry method for elemental characterization of the Hanford Tank Waste Treatment and Immobilization Plant (WTP) pretreated low activity waste (LAW) stream to the LAW Vitrification Plant. The WTP is evaluating the potential for using XRF as a rapid turnaround technique to support LAW product compliance and glass former batching. The overall objective of this task was to develop XRF analytical methods that provide the rapid turnaround time (<8 hours) requested by the WTP, while providing sufficient accuracy and precision to determine waste composition variations. For Phase 1a, SRNL (1) evaluated, selected, and procured an XRF instrument for WTP installation, (2) investigated three XRF sample methods for preparing the LAW sub-sample for XRF analysis, and (3) initiated scoping studies on AN-105 (Envelope A) simulant to determine the instrument's capability, limitations, and optimum operating parameters. After preliminary method development on simulants and the completion of Phase 1a activities, SRNL received approval from WTP to begin Phase 1b activities with the objective of optimizing the XRF methodology. Three XRF sample methods used for preparing the LAW sub-sample for XRF analysis were studied: direct liquid analysis, dried spot, and fused glass. The direct liquid method was selected because its major advantage is that the LAW can be analyzed directly without any sample alteration that could bias the method accuracy. It also is the fastest preparation technique--a typical XRF measurement could be completed in < 1hr after sample delivery. Except for sodium, the method detection limits (MDLs) for the most important analytes in solution, the hold point elements, were achieved by this method. The XRF detection limits are generally adequate for glass former batching and product composition reporting, but may be inadequate for some species (Hg, Cd, and Ba) important to land disposal restrictions. The long term precision (24-hr) also was good with percent relative standard deviations (%RSDs) < 10 % for most elements in filtered solution. There were some issues with a few elements precipitating out of solution over time affecting the long term precision of the method. Additional research will need to be performed to resolve this sample stability problem. Activities related to methodology optimization in the Phase 1b portion of the study were eliminated as a result of WTP request to discontinue remaining activities due to funding reduction. These preliminary studies demonstrate that developing an XRF method to support the LAW vitrification plant is feasible. When funding is restored for the WTP, it is recommended that optimization of this technology should be pursued.

Jurgensen, A; David Missimer, D; Ronny Rutherford, R

2006-05-08T23:59:59.000Z

462

Fluorescence-type Monochromatic X-ray Beam-position Monitor with High-spatial Resolution for the NSLS-II Beamlines  

SciTech Connect (OSTI)

We developed a fluorescence-type monochromatic X-ray beam-position monitor (X-BPM) with high-spatial resolution for end-station experiments at the initial project beamlines of the NSLS-II. We designed a ring array of multi-segmented Si PIN-junction photodiodes to use as a position sensor. Further, we integrated a low-noise charge-preamplification HERMES4 ASIC chip into an electronic readout system for photon-counting application. A series of precision measurements to characterize electronically the Si-photodiode sensor and the ASIC chip demonstrated that the inherent noise from the detector system is sufficiently low to meet our stringent requirements. Using a Gaussian beam, we parametrically modeled the optimum working distance to ensure the detector's best performance. Based upon the results from the parametric modeling, prototypes of the next versions of the X-BPM are being developed. In this paper, we describe the methodology for developing the new compact monochromatic X-ray BPM, including its instrumentation, detector modeling, and future plan.

Yoon, Phil S. [Experimental Facility Division, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973 (United States); Siddons, D. Peter [Experimental Systems, NSLS, Brookhaven National Laboratory, Upton, NY 11973 (United States)

2010-06-23T23:59:59.000Z

463

Flash photolysis resonance fluorescence investigation of the gas-phase reactions of hydroxyl radicals with cyclic ethers  

SciTech Connect (OSTI)

Absolute rate constants were measured for the gas-phase reactions of hydroxyl radicals with a series of dioxanes and other cyclic ethers by using the flash photolysis resonance fluorescence technique. Kinetic data for 1,3-dioxane and 1,4-dioxane, reactions 1 and 2, over the temperature range 240-440 K were used to derive the Arrhenius expressions. These results are compared to our earlier measurements for aliphatic ethers and are discussed in terms of reaction mechanisms and the prediction of reaction rates for such compounds from group reactivity values.

Dagaut, P.; Liu, R.; Wallington, T.J.; Kurylo, M.J. (National Institute of Standards and Technology, Gaithersburg, MD (USA))

1990-03-08T23:59:59.000Z

464

2014-12-30 Issuance: Energy Conservation Standard for General Service Fluorescent Lamps and Incandescent Reflector Lamps; Final Rule  

Broader source: Energy.gov [DOE]

This document is a pre-publication Federal Register final rule regarding energy conservation standards for general service fluorescent lamps and incandescent reflector lamps, as issued by the Deputy Assistant Secretary for Energy Efficiency on December 30, 2014. Though it is not intended or expected, should any discrepancy occur between the document posted here and the document published in the Federal Register, the Federal Register publication controls. This document is being made available through the Internet solely as a means to facilitate the public's access to this document.

465

Validation of a comercially available fluorescence-based instrument to evaluate stallion spermatozoal concentration and comparison to photometric systems  

E-Print Network [OSTI]

ejaculated and extended spermatozoa. The instrument is also more repeatable than either photometric system, but may be cost-prohibitive for some operations. v v ACKNOWLEDGEMENTS The author wishes to thank ChemoMetec A/S of Aler?d, Denmark... in commercial breding operations because they are more afordable than a flow cytometer and produce results more quickly than a hemacytometer. ChemoMetec A/S (Aler?d, Denmark) has developed the NucleoCounter SP-100, a fluorescence-based instrument...

Comerford, Kathryn L.

2010-01-16T23:59:59.000Z

466

Ultra-Fast Fluorescence Imaging in Vivo with Conjugated Polymer Fluorophores in the Second Near-Infrared Window  

E-Print Network [OSTI]

In vivo fluorescence imaging in the second near-infrared window (1.0-1.7 microns) can afford deep tissue penetration and high spatial resolution, owing to the reduced scattering of long-wavelength photons. Here, we synthesize a series of low-bandgap donor/acceptor copolymers with tunable emission wavelengths of 1050-1350 nm in this window. Non-covalent functionalization with phospholipid-polyethylene glycol results in water-soluble and biocompatible polymeric nanoparticles, allowing for live cell molecular imaging at > 1000 nm with polymer fluorophores for the first time. Importantly, the high quantum yield of the polymer allows for in vivo, deep-tissue and ultrafast imaging of mouse arterial blood flow with an unprecedented frame rate of > 25 frames per second. The high time resolution results in spatially and time resolved imaging of the blood flow pattern in cardiogram waveform over a single cardiac cycle (~ 200 ms) of a mouse, which has not been observed with fluorescence imaging in this window before.

Hong, Guosong; Antaris, Alexander L; Diao, Shuo; Wu, Di; Cheng, Kai; Zhang, Xiaodong; Chen, Changxin; Liu, Bo; He, Yuehui; Wu, Justin Z; Yuan, Jun; Zhang, Bo; Tao, Zhimin; Fukunaga, Chihiro; Dai, Hongjie

2014-01-01T23:59:59.000Z

467

2-dimensional ion velocity distributions measured by laser-induced fluorescence above a radio-frequency biased silicon wafer  

SciTech Connect (OSTI)

The dynamics of ions traversing sheaths in low temperature plasmas are important to the formation of the ion energy distribution incident onto surfaces during microelectronics fabrication. Ion dynamics have been measured using laser-induced fluorescence (LIF) in the sheath above a 30 cm diameter, 2.2 MHz-biased silicon wafer in a commercial inductively coupled plasma processing reactor. The velocity distribution of argon ions was measured at thousands of positions above and radially along the surface of the wafer by utilizing a planar laser sheet from a pulsed, tunable dye laser. Velocities were measured both parallel and perpendicular to the wafer over an energy range of 0.4600 eV. The resulting fluorescence was recorded using a fast CCD camera, which provided resolution of 0.4 mm in space and 30 ns in time. Data were taken at eight different phases during the 2.2 MHz cycle. The ion velocity distributions (IVDs) in the sheath were found to be spatially non-uniform near the edge of the wafer and phase-dependent as a function of height. Several cm above the wafer the IVD is Maxwellian and independent of phase. Experimental results were compared with simulations. The experimental time-averaged ion energy distribution function as a function of height compare favorably with results from the computer model.

Moore, Nathaniel B.; Gekelman, Walter; Pribyl, Patrick [Department of Physics and Astronomy, University of California, Los Angeles, California 90095 (United States)] [Department of Physics and Astronomy, University of California, Los Angeles, California 90095 (United States); Zhang, Yiting; Kushner, Mark J. [Department of Electrical Engineering and Computer Science, University of Michigan, 1301 Beal Ave., Ann Arbor, Michigan 48109-2122 (United States)] [Department of Electrical Engineering and Computer Science, University of Michigan, 1301 Beal Ave., Ann Arbor, Michigan 48109-2122 (United States)

2013-08-15T23:59:59.000Z

468

Determination of the properties of nuclear energy levels of La? and Pr? by the resonance fluorescence of neutron capture gamma rays  

E-Print Network [OSTI]

DE ERMINATION OF THE PROPERTIES OF NUCLEAR ENEPGY LEVELS OF La~ AND Pr-"' BY THE RESCNANCE FLUORESCENCE CF Ni". UTRON CAPTURE BAI'IMA RAYS A Thesis William Bradley Wilson Submitted to the Graduate College of Texas ASM University in partial... fulfil'men o. the reouirement for ti. e degr e of Master of Science January, 1969 MaJ'or Sub) ect Nuclea Eng. '. u:eering DE' EPM1INATION OF THE PROPERTIES OF NUCLEAR ENERGY LEVELS OF La ~ AND Pr'" BY THE RFSONANCE FLUORESCENCE OF NEUTRON CAP URE...

Wilson, William Bradley

2012-06-07T23:59:59.000Z

469

Synthesis of Fluorescent C2-Bridged Teraryls and Quateraryls for Blue, Sky-Blue, and Green Color Light-Emitting Devices  

Science Journals Connector (OSTI)

Synthesis of Fluorescent C2-Bridged Teraryls and Quateraryls for Blue, Sky-Blue, and Green Color Light-Emitting Devices ... UVvis, fluorescence spectra, and cyclic voltammograms of 6ae and 9ac; atom coordinates and absolute energies and TGA plots of 6a, and 9a,b; X-ray data of 6b; NMR spectra of 3c,e, 4ac, 4e, 6ae, and 9ac. ...

Atul Goel; Ashutosh Sharma; Madhu Rawat; R. S. Anand; Ruchir Kant

2014-10-23T23:59:59.000Z

470

Final Report for Nuclear Resonance Fluorescence Measurements of 239Pu above 2.5 MeV  

SciTech Connect (OSTI)

Nuclear Resonance Fluorescence measurements were performed at the free electron laser facility at UC Santa Barbara using a bremsstrahlung beam. Three endpoint energies were chosen for the bremsstrahlung to cover as much area above 2.5 MeV as possible. We were able to set an upper limit of NRF state strengths between 2.5 and 3.8 MeV at roughly 38(5) eV barns at the 4-sigma level and 9(2) eV barns at the 1-sigma level. Published results on states near 2.4 MeV indicate strengths about 10(2) eV barns. Details of the results are presented in this report.

Johnson, M S; McNabb, D P

2009-01-07T23:59:59.000Z

471

Fluorescence properties and electron paramagnetic resonance studies of {gamma}-irradiated Sm{sup 3+}-doped oxyfluoroborate glasses  

SciTech Connect (OSTI)

The permanent photoinduced valence manipulation of samarium doped oxyfluoroborate glasses as a function of {gamma}-ray irradiation has been investigated using a steady-state fluorescence and electron paramagnetic resonance techniques. An increase in SrF{sub 2} content in the glass led to the red shift of the peaks in as prepared glass, while in irradiated glasses this led to the decrease in defect formation as well as increase in photoreduction of Sm{sup 3+} to Sm{sup 2+} ion. The energy transfer mechanism of induced permanent photoreduction of Sm{sup 3+} to Sm{sup 2+} ions in oxyfluoroborate glasses has been discussed. The decay analysis shows exponential behavior before irradiation and non-exponential behavior after irradiation. The energy transfer in irradiated glasses increases with the increase in SrF{sub 2} content in the glass and also with the irradiation dose.

Babu, B. Hari; Ravi Kanth Kumar, V. V. [Department of Physics, Pondicherry University, Kalapet, Puducherry-605 014 (India)

2012-11-01T23:59:59.000Z

472

Brain Uptake of a Fluorescent Vector Targeting the Transferrin Receptor: A Novel Application of in Situ Brain Perfusion  

Science Journals Connector (OSTI)

Brain Uptake of a Fluorescent Vector Targeting the Transferrin Receptor: A Novel Application of in Situ Brain Perfusion ... Next, brains were collected and transferred into ice-cold 0.1 M PBS, where cerebellum, meninges, brain stem, and large superficial blood vessels were removed. ... Using the in situ brain perfusion technique, we showed that GF120918 (dual inhibitor of Abcb1 and Abcg2) strongly enhanced the uptake (Clup, ?l/g/s) of [3H] A?1-40 by the brains of Abcb1-deficient mice, but not by the brains of Abcb1/Abcg2-deficient mice, suggesting that Abcg2 is involved in the transport of A? at the mouse BBB. ...

Wael Alata; Sarah Paris-Robidas; Vincent Emond; Fanchon Bourasset; Frdric Calon

2013-11-11T23:59:59.000Z

473

Time-Resolved Emission Study of a Thiophene-Modified Fluorescent Nucleoside in Solution and within Multiply-Modified Oligodeoxynucleotides  

E-Print Network [OSTI]

Steady-state and time-resolved emission techniques were employed to study the photophysical properties of 5-(thien-2-yl)-2'-deoxyuridine (dUTh), an isomorphic fluorescent nucleoside analog. We found that the emission lifetime of dUTh is dependent upon the solvent viscosity and obeys the F\\"orster-Hoffman relation over a wide range of temperatures in 1-propanol, a glass-forming liquid. Upon incorporation into oligodeoxynucleotides, the average emission lifetime significantly increases, and the decay is non-exponential. We use a non-radiative decay model that takes into account the twist angle of the thiophene ring to fit the time-resolved emission decay curves.

Mary Noe; Yuval Erez; Itay Presiado; Yitzhak Tor; Dan Huppert

2014-12-15T23:59:59.000Z

474

Large-area, low-noise, high-speed, photodiode-based fluorescence detectors with fast overdrive recovery  

SciTech Connect (OSTI)

Two large-area, low-noise, high-speed fluorescence detectors have been built. One detector consists of a photodiode with an area of 28 mmx28 mm and a low-noise transimpedance amplifier. This detector has a input light-equivalent spectral noise density of less than 3 pW/{radical}(Hz), can recover from a large scattered light pulse within 10 {mu}s, and has a bandwidth of at least 900 kHz. The second detector consists of a 16-mm-diam avalanche photodiode and a low-noise transimpedance amplifier. This detector has an input light-equivalent spectral noise density of 0.08 pW/{radical}(Hz), also can recover from a large scattered light pulse within 10 {mu}s, and has a bandwidth of 1 MHz.

Bickman, S.; DeMille, D. [Physics Department, Yale University, P.O. Box 208120, SPL 23, New Haven, Connecticut 06520 (United States)

2005-11-15T23:59:59.000Z

475

An X-Ray Fluorescence Study on the Segregation of Cs and I in and Inverted Organic Solar Cell  

SciTech Connect (OSTI)

X-ray near-total-reflection fluorescence reveals that in multilayers of the inverted organic solar cell (ITO/CsI/P3HT:PCBM-based) Cs diffuses into the organic layer and iodide diffuses into the ITO. Laser ablation inductively coupled plasma mass spectrometry measurements, which integrate elemental concentration across the whole multilayer structure, indicate that the Cs:I ratio remains 1:1 confirming there is no loss of iodine from the sample. Iodide diffusion to the bulk ITO layer is also found in a similarly prepared ITO/NaI/P3HT:PCBM multilayer structure. Our results are consistent with recent XPS measurements which show that the Cs:I ratio at the ITO/CsI surface exceeds 8:1, and rationalize this observation.

Lindemann, William R. [Ames Laboratory; Xiao, Teng [Ames Laboratory; Wang, Wenjie [Ames Laboratory; Berry, Jonna E. [Ames Laboratory; Anderson, Nathaniel A. [Ames Laboratory; Houk, Robert S. [Ames Laboratory; Shinar, Ruth [Ames Laboratory; Shinar, Joseph [Ames Laboratory; Vaknin, David [Ames Laboratory

2013-10-08T23:59:59.000Z

476

Time-synchronized continuous wave laser-induced fluorescence axial velocity measurements in a diverging cusped field thruster  

Science Journals Connector (OSTI)

Measurements are presented of time-synchronized axial ion velocities at three positions in the discharge channel and plume of a diverging cusped field thruster operating on xenon. Xenon axial ion velocities for the thruster are derived from laser-induced fluorescence measurements of the 5d[4]7/26p[3]5/2 xenon ion excited state transition centred at ?=834.72nm. The thruster is operated in a high-current mode, where the anode discharge current is shown to oscillate quasi-periodically. A sample-hold scheme is implemented to correlate ion velocities to phases along the current cycle. These time-synchronized measurements show that median axial ion velocities decrease as discharge current increases, and that the widths of ion velocity distributions increase with increases in discharge current for positions at the exit plane and outside the thruster channel.

N A MacDonald; M A Cappelli; W A Hargus Jr

2014-01-01T23:59:59.000Z

477

Cavity induced modifications to the resonance fluorescence and probe absorption of a laser-dressed V atom  

E-Print Network [OSTI]

A cavity-modified master equation is derived for a coherently driven, V-type three-level atom coupled to a single-mode cavity in the bad cavity limit. We show that population inversion in both the bare and dressed-state bases may be achieved, originating from the enhancement of the atom-cavity interaction when the cavity is resonant with an atomic dressed-state transition. The atomic populations in the dressed state representation are analysed in terms of the cavity-modified transition rates. The atomic fluorescence spectrum and probe absorption spectrum also investigated, and it is found that the spectral profiles may be controlled by adjusting the cavity frequency. Peak suppression and line narrowing occur under appropriate conditions.

Jin-Sheng Peng; Gao-Xiang Li; Peng Zhou; S. Swain

2000-03-20T23:59:59.000Z

478

Use of Modern Chemical Protein Synthesis and Advanced Fluorescent Assay Techniques to Experimentally Validate the Functional Annotation of Microbial Genomes  

SciTech Connect (OSTI)

The objective of this research program was to prototype methods for the chemical synthesis of predicted protein molecules in annotated microbial genomes. High throughput chemical methods were to be used to make large numbers of predicted proteins and protein domains, based on microbial genome sequences. Microscale chemical synthesis methods for the parallel preparation of peptide-thioester building blocks were developed; these peptide segments are used for the parallel chemical synthesis of proteins and protein domains. Ultimately, it is envisaged that these synthetic molecules would be printed in spatially addressable arrays. The unique ability of total synthesis to precision label protein molecules with dyes and with chemical or biochemical tags can be used to facilitate novel assay technologies adapted from state-of-the art single molecule fluorescence detection techniques. In the future, in conjunction with modern laboratory automation this integrated set of techniques will enable high throughput experimental validation of the functional annotation of microbial genomes.

Kent, Stephen [University of Chicago

2012-07-20T23:59:59.000Z

479

Reconstruction of Longitudinal Profiles of Ultra-High Energy Cosmic Ray Showers from Fluorescence and Cherenkov Light Measurements  

E-Print Network [OSTI]

We present a new method for the reconstruction of the longitudinal profile of extensive air showers induced by ultra-high energy cosmic rays. In contrast to the typically considered shower size profile, this method employs directly the ionization energy deposit of the shower particles in the atmosphere. Due to universality of the energy spectra of electrons and positrons, both fluorescence and Cherenkov light can be used simultaneously as signal to infer the shower profile from the detected light. The method is based on an analytic least-square solution for the estimation of the shower profile from the observed light signal. Furthermore, the extrapolation of the observed part of the profile with a Gaisser-Hillas function is discussed and the total statistical uncertainty of shower parameters like total energy and shower maximum is calculated.

M. Unger; B. R. Dawson; R. Engel; F. Schssler; R. Ulrich

2008-01-28T23:59:59.000Z

480

A fluorescence method to detect photo-activated repair of UV-induced DNA damage in marine organisms  

SciTech Connect (OSTI)

After UV exposure, marine microorganisms may re-activate their photosynthetic systems or regain their pre-exposure metabolic rates, but they will not long survive, nor grow or reproduce, if they cannot repair DNA or ERN damage. Several repair mechanisms, including nucleotide excision repair and photo-reactivation, are known in terrestrial organisms, but there is little information on either process in marine microorganisms. Photo-reactivation may be especially important near the ocean surface where a few meters difference in vertical position or a few hours difference in time may alleviate UV-irradiation stress yet still provide sufficient light for photoactivated repair processes. We have developed a relatively simple, sensitive method to measure the activity of the photo-reactivation enzyme, photolyase, by fluorometry. UV irradiation causes adjacent thymine nucleotides in DNA to form cyclobutane dimers. Photolyase attaches to the dimerized DNA then breaks the dimers in a reaction catalyzed by visible light, thereby allowing restoration of cross-strand base pairing. Our assay for photolyase activity involves a dimerized synthetic DNA and DNA-specific fluorochromes with affinity for thymine-rich regions of DNA. When thymine dimers are present, fluorescence of these dyes is reduced. As dimers are repaired, fluorescence increases, allowing us to demonstrate the presence of photolyase and measure its activity using conventional fluorometers available in most marine laboratories. Photo-reactivation activity has been unambiguously demonstrated in samples of coastal seawater. In preliminary investigations, cells taken from surface slicks did not have enhanced photo-repair capabilities relative to cells collected 5 meters below the surface. Additional tests of the method and measurements of photo-repair activities of surface and subsurface organisms will be made during the SLIX-89 Surface Microlayer Experiment in October 1989.

Carlson, D.J.; Mordy, C.W.; Nelson, K. (Oregon State Univ., Corvallis (United States))

1990-01-09T23:59:59.000Z

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481

A fast and sensitive method for the determination of nitrite in human plasma by capillary electrophoresis with fluorescence detection  

Science Journals Connector (OSTI)

Analysis of nitrite, the indicator of nitric oxide (NO) generation in vivo, provides a useful tool to study NO synthesis in vivo. A fast and sensitive fluorometric CE method was developed for determination of nitrite in human plasma through its derivatization with 2,3-diaminonaphthalene (DAN). Nitrite in human plasma was easily reacted with DAN under acid conditions to yield the highly fluorescent 2,3-naphthotriazole (NAT). Fluorescence detection was optimized to achieve subnanomolar detection which allows a direct analysis of plasma samples unlike most CEUV methods using sample stacking. Acetonitrile was used to remove the protein. Short-end injection and a high voltage (?30kV) were used to shorten the analysis time. The good separation was achieved with 20mM borate buffer at pH 9.23. The separation of NAT was obtained within 1.4min. The deproteinized plasma sample was injected hydrodynamically for 5s at ?50mbar into a 60cm75?m internal diameter uncoated fused-silica capillary. Excitation wavelength was selected with a broad-band filter (240400nm), and the emitted light was measured at 418nm by the use of a cutoff filter. A good linearity (R2=0.9975) was obtained in the range from 2 to 500nM. The detection limit of nitrite was 0.6nM in original plasma samples, which is 750 times lower than our previous CEUV method. The developed fluorometric CE method offers the advantages of more simple system and lower cost compared with the current fluorometric HPLC methods without losing sensitivity. The detected mean nitrite concentration in human plasma by this method was consistent with the most frequently reported values.

Xu Wang; Erwin Adams; Ann Van Schepdael

2012-01-01T23:59:59.000Z

482

Conclusion: We described an efficient non-invasive benign Methods: We use fluorescent silica colloidal particles of method to quantify dynamics and to perform mapping of  

E-Print Network [OSTI]

temperature (1). The air (7). skin has three major layers: the epidermis, the Different skin care products products, we use two base materials: either glycerin or vaseline. A mixture of each Key words: skin care products product with fluorescent particles is applied on human skin. spectroscopy The amount

Sokolov, Igor

483

Detailed modeling and laser-induced fluorescence imaging of nitric oxide in a NH3-seeded non-premixed methane/air flame  

E-Print Network [OSTI]

non-premixed methane/air flame John B. Bell, Marcus S. Day, Joseph F. Grcar Computing Sciences-induced fluorescence imaging of nitric oxide in a NH3-seeded non-premixed methane/air flame Abstract In this paper we study the formation of NO in laminar, nitrogen diluted methane diffusion flames that are seeded

Bell, John B.

484

Study of Rb atomic transitions D1,2 lines in strong magnetic field based on fluorescence spectra of sub -micron thin cell  

E-Print Network [OSTI]

Study of Rb atomic transitions D1,2 lines in strong magnetic field based on fluorescence spectra "forbidden" transitions in magnetic field B are detected and studied. Also, on 87 Rb D1 line, Fg = 1, mF = 0 the levels of the hyperfine structure of the D1,2 lines of 87 Rb atoms in magnetic fields with B varying

Boyer, Edmond

485

Modified Atmosphere Packaged Cheddar Cheese Shreds:? Influence of Fluorescent Light Exposure and Gas Type on Color and Production of Volatile Compounds  

Science Journals Connector (OSTI)

Modified Atmosphere Packaged Cheddar Cheese Shreds:? Influence of Fluorescent Light Exposure and Gas Type on Color and Production of Volatile Compounds ... The cheese block was shredded with a hand shredder. ... Aldehydes were the major constituent of the volatile fraction of shredded Cheddar cheese packaged under CO2. ...

Llori M. Colchin; Sandra L. Owens; Galina Lyubachevskaya; Elizabeth Boyle-Roden; Estelle Russek-Cohen; Scott A. Rankin

2001-04-13T23:59:59.000Z

486

Elimination of self-absorption in fluorescence hard-x-ray absorption spectra P. Pfalzer, J.-P. Urbach, M. Klemm, and S. Horn  

E-Print Network [OSTI]

Elimination of self-absorption in fluorescence hard-x-ray absorption spectra P. Pfalzer, J-ray absorption spectra in situations where samples cannot be made in the required configuration. However, self-absorption-ray absorption coefficients. This procedure is used to obtain the vanadium K-edge spectrum of single crystal V2O3

Frenkel, Anatoly

487

Application of synchrotron radiation X-ray fluorescence imaging combined with histochemical staining to the renal section of mercury-treated rats  

Science Journals Connector (OSTI)

A biomedical application of synchrotron radiation comprising X-ray fluorescence imaging and histochemical staining was employed to examine the detailed distribution of metal elements and morphological changes in the kidney section of mercury-treated rats. The spatial resolution was improved to 5 5 m2.

Homma-Takeda, S.

1998-01-01T23:59:59.000Z

488

Electronic structure of the 4d transition metal carbides: Dispersed fluorescence spectroscopy of MoC, RuC, and PdC  

E-Print Network [OSTI]

Electronic structure of the 4d transition metal carbides: Dispersed fluorescence spectroscopy of Mo transition metal carbides is also provided. © 2001 American Institute of Physics. DOI: 10.1063/1.1316042 I, and astrochemistry. Within the 4d se- ries, the diatomic transition metal carbides have aroused considerable interest

Morse, Michael D.

489

Laser-induced fluorescence measurements and kinetic analysis of Si atom formation in a rotating disk chemical vapor deposition reactor  

SciTech Connect (OSTI)

An extensive set of laser-induced fluorescence (LIF) measurements of Si atoms during the chemical vapor deposition (CVD) of silicon from silane and disilane in a research rotating disk reactor are presented. The experimental results are compared in detail with predictions from a numerical model of CVD from silane and disilane that treats the fluid flow coupled to gas-phase and gas-surface chemistry. The comparisons showed that the unimolecular decomposition of SiH[sub 2] could not account for the observed gas-phase Si atom density profiles. The H[sub 3]SiSiH [leftrightarrow] Si + SiH[sub 4] and H[sub 3]SiSiH + SiH[sub 2] [leftrightarrow] Si + Si[sub 2]H[sub 6] reactions are proposed as the primary Si atom production routes. The model is in good agreement with the measured shapes of the Si atom profiles and the trends in Si atom density with susceptor temperature, pressure, and reactant gas mixture. 33 refs., 12 figs., 3 tabs.

Ho, P.; Coltrin, M.E.; Breiland, W.G. (Sandia National Lab., Albuquerque, NM (United States))

1994-10-06T23:59:59.000Z

490

X-ray microprobe synchroton radiation X-ray fluorescence application on human teeth of renal insufficiency patients  

Science Journals Connector (OSTI)

This work reports on the measurements of elemental profiles in teeth collected from patients with renal insufficiency. Elemental concentrations of Ti, Mn, Fe, Co, Ni, Cu, Zn, Se, Br, Rb Sr and Pb in different parts of teeth from patients with renal insufficiency are discussed and correlated with the corresponding values for healthy citizens. Both situations, patients with and without dialysis treatment were studied. The purpose of this work is to point out the influence of renal insufficiency together with long dialysis treatment, on teeth elemental content. An X-ray fluorescence set-up with microprobe capabilities, installed at the LURE synchrotron (France) was used for elemental determination. The resolution of the synchrotron microprobe was 100 ?m and the energy of the incident photons was 19 keV. Teeth of citizens with renal insufficiency and those submitted since several years to dialysis treatment show a similar concentration with teeth of healthy subjects in what concerns the elemental distribution for Mn, Fe, Cu, Zn and Sr. However, higher levels of Pb were found in pulp region of diseased citizens when compared to values of healthy people. Very low concentrations of Ti, Co, Ni, Se, Br and Rb were found in all the analysed teeth. No difference was found in patients with and without dialysis treatment.

A.F. Marques; J.P. Marques; C. Casaca; M.L. Carvalho

2004-01-01T23:59:59.000Z

491

Pigments Elementary Chemical Composition Study of a Gainsborough Attributed Painting Employing a Portable X-Rays Fluorescence System  

SciTech Connect (OSTI)

The investigated painting, identified with the title 'The woodman', is attributed to Thomas Gainsborough (XVIII century) and is under investigation at the Laboratory of Conservation Science (LACICOR), CECOR/EBA/UFMG. The measurements were carried out with a portable X-rays fluorescence (XRF) system constituted of a X-rays tube with Ag anode, a Si PIN - diode detector, nuclear electronic chain and a special designed mechanical system for the detector and X-ray tube positioning, that enables angular and XYZ movements of the excitation-detection system. The employed voltage and current intensity were 17 kV and 3 mA, respectively. The time of acquisition for each measurement was 500 s. XRF spectra were analyzed using the AXIL-WinQXAS software. Three measurements in each of the following regions of the painting were done: face, leaves, arm, sky and firewood. The carried out analysis indicated the following pigments: White (lead white and calcium sulfate, identified by the elements Pb, Ca and S), Blue (Prussian blue, identified by the key element Fe), Red (Vermilion, identified by the elements Hg and S) and Brown (mixture of Fe and Mn oxides, identified by the elements Fe and Mn). Elements belonging to modern pigments corresponding to the same colors were absent in the analyzed spectra.

Appoloni, C. R.; Blonski, M. S. [State University of Londrina, Department of Physics, Londrina, PR (Brazil); Parreira, P. S.; Souza, L. A. C. [Federal University of Minas Gerais, EBA/CECOR/LACICOR Belo Horizonte, MG (Brazil)

2007-02-12T23:59:59.000Z

492

Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells  

SciTech Connect (OSTI)

The presented green fluorescent protein and streptavidin core-based tripartite fusion system provides a simple and efficient way for the production of proteins fused to it in insect cells. This fusion protein forms a unique tag, which serves as a multipurpose device enabling easy optimization of production, one-step purification via streptavidin-biotin interaction, and visualization of the fusion protein during downstream processing and in applications. In the present study, we demonstrate the successful production, purification, and detection of a natural rubber latex allergen Hev b5 with this system. We also describe the production of another NRL allergen with the system, Hev b1, which formed large aggregates and gave small yields in purification. The aggregates were detected at early steps by microscopical inspection of the infected insect cells producing this protein. Therefore, this fusion system can also be utilized as a fast indicator of the solubility of the expressed fusion proteins and may therefore be extremely useful in high-throughput expression approaches.

Nordlund, Henri R. [Department of Biological and Environmental Science, NanoScience Center, P.O. Box 35, FIN-40014 University of Jyvaeskylae (Finland)]. E-mail: henri.nordlund@uta.fi; Laitinen, Olli H. [AI Virtanen Institute, Department of Molecular Medicine, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio (Finland); Uotila, Sanna T.H. [Department of Biological and Environmental Science, NanoScience Center, P.O. Box 35, FIN-40014 University of Jyvaeskylae (Finland); Kulmala, Minna [Institute of Biotechnology, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FIN-00014 Helsinki (Finland); Kalkkinen, Nisse [Institute of Biotechnology, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FIN-00014 Helsinki (Finland); Kulomaa, Markku S. [Department of Biological and Environmental Science, NanoScience Center, P.O. Box 35, FIN-40014 University of Jyvaeskylae (Finland)

2005-10-14T23:59:59.000Z

493

Detection of dark galaxies and circum-galactic filaments fluorescently illuminated by a quasar at z=2.4  

E-Print Network [OSTI]

A deep narrow-band survey for Ly-alpha emission carried out on the VLT-FORS2 has revealed 98 Ly-alpha candidates down to a flux limit of 4.e-18 erg/s/cm^2 in a volume of 5500 comoving Mpc^3 at z=2.4 centered on the hyperluminous quasar HE0109-3518. The properties of the detected sources in terms of their i) equivalent width distribution, ii) luminosity function, and iii) the average luminosity versus projected distance from the quasar, all suggest that a large fraction of these objects have been fluorescently "illuminated" by HE0109-3518. This conclusion is supported by comparison with detailed radiative transfer simulations of the effects of the quasar illumination. 18 objects have a rest-frame Equivalent Width (EW0) larger than 240A, the expected limit for Ly-alpha emission powered by Population II star formation and 12 sources among these do not have any continuum counterpart in a deep V-band imaging of the same field. For these, a stacking analysis indicates EW0>800A, effectively ruling out Ly-alpha power...

Cantalupo, Sebastiano; Haehnelt, Martin G

2012-01-01T23:59:59.000Z

494

Fluorescence enhancement of CdTe MPA-capped quantum dots by glutathione for hydrogen peroxide determination  

Science Journals Connector (OSTI)

Abstract The manipulation of the surface chemistry of semiconductor nanocrystals has been exploited to implement distinct sensing strategies in many analytical applications. In this work, reduced glutathione (GSH) was added at reaction time, as an electron-donor ligand, to markedly increase the quantum yield and the emission efficiency of MPA-capped CdTe quantum dots. The developed approach was employed in the implementation of an automated flow methodology for hydrogen peroxide determination, as this can oxidize GSH preventing its surface passivating effect and producing a manifest fluorescence quenching. After optimization, linear working calibration curve for hydrogen peroxide concentrations between 0.0025% and 0.040% were obtained (n=6), with a correlation coef?cient of 0.9975. The detection limit was approximately 0.0012%. The developed approach was employed in the determination of H2O2 in contact lens preservation solutions and the obtained results complied with those furnished by the reference method, with relative deviations comprised between ?1.18 and 4.81%.

S. Sofia M. Rodrigues; David S.M. Ribeiro; L. Molina-Garcia; A. Ruiz Medina; Joo A.V. Prior; Joo L.M. Santos

2014-01-01T23:59:59.000Z

495

DOE/EA-1664: Environmental Assessment for 10 CFR 430 Energy Conservation Standards: Energy Conservation Standards for Fluorescent and Incandescent Lamps (June 2009)  

Broader source: Energy.gov (indexed) [DOE]

4 4 Environmental Assessment for 10 CFR Part 430 Energy Conservation Standards: Energy Conservation Standards for Fluorescent and Incandescent Lamps June 2009 16-i CHAPTER 16. ENVIRONMENTAL ASSESSMENT TABLE OF CONTENTS 16.1 INTRODUCTION ......................................................................................................... 16-1 16.2 AIR EMISSIONS ANALYSIS...................................................................................... 16-1 16.2.1 Air Emissions Descriptions............................................................................................ 16-1 16.2.2 Air Quality Regulation................................................................................................... 16-3 16.2.3 Global Climate Change..................................................................................................

496

Green and Orange CdTe Quantum Dots as Effective pH-Sensitive Fluorescent Probes for Dual Simultaneous and Independent Detection of Viruses  

Science Journals Connector (OSTI)

Green and Orange CdTe Quantum Dots as Effective pH-Sensitive Fluorescent Probes for Dual Simultaneous and Independent Detection of Viruses ... 26 The F0F1-ATP synthase is a nanoscale rotary biological motor. ... On the basis of this primary model, more than three different kinds of QDs could be mixed together to construct multicolor QD biosensors, which may be a feasible method to optical encoding detection of various viruses in the future. ...

Zhengtao Deng; Yun Zhang; Jiachang Yue; Fangqiong Tang; Qun Wei

2007-09-21T23:59:59.000Z

497

Air fluorescence measurements in the spectral range 300-420 nm using a 28.5 GeV electron beam  

E-Print Network [OSTI]

Measurements are reported of the yield and spectrum of fluorescence, excited by a 28.5 GeV electron beam, in air at a range of pressures of interest to ultra-high energy cosmic ray detectors. The wavelength range was 300 - 420 nm. System calibration has been performed using Rayleigh scattering of a nitrogen laser beam. In atmospheric pressure dry air at 304 K the yield is 20.8 +/- 1.6 photons per MeV.

R. Abbasi; T. Abu-Zayyad; K. Belov; J. Belz; Z. Cao; M. Dalton; Y. Fedorova; P. Huentemeyer; B. F. Jones; C. C. H. Jui; E. C. Loh; N. Manago; K. Martens; J. N. Matthews; M. Maestas; J. Smith; P. Sokolsky; R. W. Springer; J. Thomas; S. Thomas; P. Chen; C. Field; C. Hast; R. Iverson; J. S. T. Ng; A. Odian; K. Reil; D. Walz; D. R. Bergman; G. Thomson; A. Zech; F-Y. Chang; C-C. Chen; C-W. Chen; M. A. Huang; W-Y. P. Hwang; G-L. Lin

2007-08-23T23:59:59.000Z

498

Experimental Security Analyses of Non-Networked Compact Fluorescent Lamps: A Case Study of Home Automation Security  

E-Print Network [OSTI]

}@uw.edu ABSTRACT Background. With a projected rise in the procurement of home automation systems, we experimentally-enabled home automation systems. Aim. This work seeks to investigate the feasibility of causing physical harm--such as through the explosion of CFLs--to home occupants through an exploited home automation system. Method. We

Kohno, Tadayoshi

499

Fluorescent sensor for mercury  

DOE Patents [OSTI]

The present invention provides a sensor for detecting mercury, comprising: a first polynucleotide, comprising a first region, and a second region, a second polynucleotide, a third polynucleotide, a fluorophore, and a quencher, wherein the third polynucleotide is optionally linked to the second region; the fluorophore is linked to the first polynucleotide and the quencher is linked to the second polynucleotide, or the fluorophore is linked to the second polynucleotide and the quencher is linked to the first polynucleotide; the first region and the second region hybridize to the second polynucleotide; and the second region binds to the third polynucleotide in the presence of Hg.sup.2+ ions.

Wang, Zidong (Urbana, IL); Lee, Jung Heon (Evanston, IL); Lu, Yi (Champaign, IL)

2011-11-22T23:59:59.000Z

500

fluorescence spectrometer | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Lee JH, JK Fredrickson, RK Kukkadapu, MI Boyanov, KM Kemner, X Lin, DW Kennedy, BN Bjornstad, A Konopka, DA Moore, CT Resch, and JL Phillips.2012."Microbial Reductive...